LOCUS       DAZGAN020000001       123543 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGAN020000001 DAZGAN020000000
VERSION     DAZGAN020000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 123543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 123543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 123543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..123543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.001"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1596)
                     /locus_tag="ACHKYO_RS00005"
                     /old_locus_tag="pgaptmp_000001"
     CDS             complement(<1..1596)
                     /locus_tag="ACHKYO_RS00005"
                     /old_locus_tag="pgaptmp_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4426913.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STPDQIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAI
                     VYKESRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHE
                     APSPIIPLNWSKASMTLMLKEMSN"
     gene            2301..2675
                     /locus_tag="ACHKYO_RS00010"
                     /old_locus_tag="pgaptmp_000002"
     CDS             2301..2675
                     /locus_tag="ACHKYO_RS00010"
                     /old_locus_tag="pgaptmp_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4426914.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDLTVVDAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
     gene            complement(3506..3898)
                     /locus_tag="ACHKYO_RS00015"
                     /old_locus_tag="pgaptmp_000003"
     CDS             complement(3506..3898)
                     /locus_tag="ACHKYO_RS00015"
                     /old_locus_tag="pgaptmp_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4426915.1"
                     /translation="MRDIQQVLERWGAWAANNRENVTWSSVAAGFKGIIPAKITSRSQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            complement(4396..4557)
                     /locus_tag="ACHKYO_RS00020"
                     /old_locus_tag="pgaptmp_000004"
     CDS             complement(4396..4557)
                     /locus_tag="ACHKYO_RS00020"
                     /old_locus_tag="pgaptmp_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4426916.1"
                     /translation="MTERSPTEIIHGIIEEQEERELILMIEEQRERQRKQEATQPDSV
                     SAENQADVT"
     gene            complement(5082..5249)
                     /locus_tag="ACHKYO_RS00025"
                     /old_locus_tag="pgaptmp_000005"
     CDS             complement(5082..5249)
                     /locus_tag="ACHKYO_RS00025"
                     /old_locus_tag="pgaptmp_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4426917.1"
                     /translation="MDTITDKKAEQLESQGLWRRAAARWLDVMKEAHTDPQREHIARR
                     REICLANFRML"
     gene            complement(5251..5553)
                     /locus_tag="ACHKYO_RS00030"
                     /old_locus_tag="pgaptmp_000006"
     CDS             complement(5251..5553)
                     /locus_tag="ACHKYO_RS00030"
                     /old_locus_tag="pgaptmp_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4426918.1"
                     /translation="MIRDDKAEALENSGLWRRAAERWGELFRQAKSDEERHYITQRRA
                     QCTAKATRPKTTEWGHVCAVRAAATRTLKDMGIDQKKEDPLRGTKYRGETGRRDGS"
     gene            5638..6024
                     /locus_tag="ACHKYO_RS00035"
                     /old_locus_tag="pgaptmp_000007"
     CDS             5638..6024
                     /locus_tag="ACHKYO_RS00035"
                     /old_locus_tag="pgaptmp_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4426919.1"
                     /translation="MRLTQYKKEILSYFTPDNLSWVRGEIGNPPFDVSGVAYLLYGID
                     SHNNRYQLESTRRTLEAMVRDGLLERVNVYERREGTGDGVRRKVARYGLPGHSVVVRD
                     GEGRNGAIDGECIRINATDVVLVDGQ"
     gene            complement(6475..6768)
                     /locus_tag="ACHKYO_RS00040"
                     /old_locus_tag="pgaptmp_000008"
     CDS             complement(6475..6768)
                     /locus_tag="ACHKYO_RS00040"
                     /old_locus_tag="pgaptmp_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4426920.1"
                     /translation="MITDSVAQKLEERGLWRRAATRCSDVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQFVTFGDVKKAADRTLKEMGINPQDEWKNYSFSDAGDDLALS"
     gene            complement(6737..7204)
                     /locus_tag="ACHKYO_RS00045"
                     /old_locus_tag="pgaptmp_000009"
     CDS             complement(6737..7204)
                     /locus_tag="ACHKYO_RS00045"
                     /old_locus_tag="pgaptmp_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HGU4426921.1"
                     /translation="MNQQTTQQDQTTRKPSRRIPWQHMTKEQRKNHRRINRIAEYWPE
                     LFGTENIKPLKAGIIRDVLEDAKSRELPLATGVLRGALTSHLHTTRYLKAIIAGGPRY
                     DLNGQPCGEVTEKEKAAASEQLMWRLEQIKQQNIQWQDTGGGESDNRQRGTET"
     gene            complement(7221..7670)
                     /locus_tag="ACHKYO_RS00050"
                     /old_locus_tag="pgaptmp_000010"
     CDS             complement(7221..7670)
                     /locus_tag="ACHKYO_RS00050"
                     /old_locus_tag="pgaptmp_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4426922.1"
                     /translation="MTAQIAAYGRLVDDPQEKQTSKGTPMALARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGQQGNQPPAGGDDPFSDDIPF"
     gene            complement(7667..7909)
                     /locus_tag="ACHKYO_RS00055"
                     /old_locus_tag="pgaptmp_000011"
     CDS             complement(7667..7909)
                     /locus_tag="ACHKYO_RS00055"
                     /old_locus_tag="pgaptmp_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4426923.1"
                     /translation="MKVSHKKTDHRAIDITEHWLRVAIKIIDRNTGEGYAKEHPELIS
                     AFMTTAAANFATLTEREIAEAEQVATINVKTGEVES"
     gene            complement(7913..8236)
                     /locus_tag="ACHKYO_RS00060"
                     /old_locus_tag="pgaptmp_000012"
     CDS             complement(7913..8236)
                     /locus_tag="ACHKYO_RS00060"
                     /old_locus_tag="pgaptmp_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4426924.1"
                     /translation="MNSDIKTLSISGALPGWWARFKDDDGTEWYSPIAAWALCEVAPC
                     NTGCAYQEILPVLPGEAGMEPHYSDCGACECLYLPDKKFVHCGESWVFAWYPVNDGSN
                     SGTLE"
     gene            complement(8702..10840)
                     /locus_tag="ACHKYO_RS00065"
                     /old_locus_tag="pgaptmp_000013"
     CDS             complement(8702..10840)
                     /locus_tag="ACHKYO_RS00065"
                     /old_locus_tag="pgaptmp_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HGU4426925.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWQEQDGKLA
                     GDKTPPVVLADEQLKDIGNLQIVPDGRKSARIYKAGHLDQVMVKGIGQKLAAAGVQDA
                     DYYPEGMHHNERQNWRNYLETERKNISDGLVIELPVKKKTSEQSEDELKPRVECRPDG
                     VYWVTPKVDKQSGEIIRPCEWIGDRMRTVGIGHDGCDGYLIIELEPEGMEKIIYEAMP
                     RNEIGLPSGWSRLRGRGVAVTTSQKLLNKLAEYLQREGERTQWEVTSTAGWHCGAYVM
                     PDGEIIGVPERPVAFCGGSAAIKGYVVRGTADEWRDNVASLMRGNHSMMLGVLVGLAA
                     PLNSLVGGGCFGVHLFAQSSAGKTTTVEAATSLYGDPEVLKLSWDATRYGLTVEAAAR
                     NDGFIPIDEIGQGGRINDIAQSAYSLFNGVGRIQGRKDGGNRAVIRWRIAALSTGEED
                     FETFLIKGGVTPKAGQLVRLLSVPFIDTTVFNGYDDGDQHARAIKRFASNYCGAAGRE
                     WIGWLSANKELAVSVVNEKENIWLSRLPENASPQVKRVASRFAMLDAAGELATGITGW
                     TTGESHAATQRAFDDWLQDFGLDNREKYQVISRARDFIQRHALSRFQPYTYGRQNGDM
                     DVNYGARITSLAGYLVRGRRDDGLPEYHIIPSVFDEEILCGINRNFGCQALKEAGILI
                     HAGDKNWTTKTIKVNGIQQRFIVLLDQSEE"
     gene            complement(11017..11208)
                     /locus_tag="ACHKYO_RS00070"
                     /old_locus_tag="pgaptmp_000014"
     CDS             complement(11017..11208)
                     /locus_tag="ACHKYO_RS00070"
                     /old_locus_tag="pgaptmp_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4426926.1"
                     /translation="MQQITLAEASLQAQQVEVISRMIERNHEEIDDHDLSVIAGLIKQ
                     LSSNVAVWLMTEEEKRGEQ"
     gene            complement(11253..11462)
                     /locus_tag="ACHKYO_RS00075"
                     /old_locus_tag="pgaptmp_000015"
     CDS             complement(11253..11462)
                     /locus_tag="ACHKYO_RS00075"
                     /old_locus_tag="pgaptmp_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4426927.1"
                     /translation="MCDFSDMTVDEAAKSAMQAELICSLMMGNTGEMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDDAICVND"
     gene            complement(11466..11651)
                     /locus_tag="ACHKYO_RS00080"
                     /old_locus_tag="pgaptmp_000016"
     CDS             complement(11466..11651)
                     /locus_tag="ACHKYO_RS00080"
                     /old_locus_tag="pgaptmp_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4426928.1"
                     /translation="MLKTFRVFSRAVNKRGYTVGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            complement(11997..12275)
                     /locus_tag="ACHKYO_RS00085"
                     /old_locus_tag="pgaptmp_000017"
     CDS             complement(11997..12275)
                     /locus_tag="ACHKYO_RS00085"
                     /old_locus_tag="pgaptmp_000017"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4426929.1"
                     /translation="MQTRLKRIAVLSQPLTTNDSKSIEAAMRNHITHPQGRDSHNLNK
                     FTWRFMQCLTGSIAIFTVTAATEREARARLPHAHLIFVARIRQEASHG"
     gene            12394..12573
                     /locus_tag="ACHKYO_RS00090"
                     /old_locus_tag="pgaptmp_000018"
     CDS             12394..12573
                     /locus_tag="ACHKYO_RS00090"
                     /old_locus_tag="pgaptmp_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4426930.1"
                     /translation="MERDHINNKSQKLQARAPHEVVEAMEQVKETGESTAQFIVTAMR
                     GEIKRRQRRKAKESE"
     gene            complement(13290..13463)
                     /locus_tag="ACHKYO_RS00095"
                     /old_locus_tag="pgaptmp_000019"
                     /pseudo
     CDS             complement(13290..13463)
                     /locus_tag="ACHKYO_RS00095"
                     /old_locus_tag="pgaptmp_000019"
                     /inference="COORDINATES: protein motif:HMM:NF019945.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
     gene            complement(13456..14175)
                     /locus_tag="ACHKYO_RS00100"
                     /old_locus_tag="pgaptmp_000020"
     CDS             complement(13456..14175)
                     /locus_tag="ACHKYO_RS00100"
                     /old_locus_tag="pgaptmp_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4426931.1"
                     /translation="MKRPNMPVQGQGFIQPEISQNDILIRHKAESRIDSRVISERAGI
                     QHESIIATIKSHQSRLRELGSLPRQSVKELTYLKSGKSGRKRGRPEISYLLNELQLDY
                     LLRVIRGRDAERINQFKLDVTKAFAKHRAAEPVRREYLPGYHESRDGLKALGAERHHY
                     INLARTENRFAGLAVGERQSAGEQQLGLLIVMQKIEQAAFNEAIQNGMTPTEAVREVA
                     RRMETFASLMTSAPSLGVNHA"
     gene            complement(14240..14437)
                     /locus_tag="ACHKYO_RS00105"
                     /old_locus_tag="pgaptmp_000021"
     CDS             complement(14240..14437)
                     /locus_tag="ACHKYO_RS00105"
                     /old_locus_tag="pgaptmp_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4426932.1"
                     /translation="MSNNLLRLSDVMRRTGYGRAWIYRLISLGRFPKPVKIGSRSVAF
                     IESEVDEWINQRIDASRNNAA"
     gene            complement(14685..15389)
                     /locus_tag="ACHKYO_RS00110"
                     /old_locus_tag="pgaptmp_000022"
     CDS             complement(14685..15389)
                     /locus_tag="ACHKYO_RS00110"
                     /old_locus_tag="pgaptmp_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4426933.1"
                     /translation="MPIVENIPDLSHWRTVQEFSITQAALLLAGIDPYDYPTLEHARD
                     YKHERWKMAWGLANGMVTAIRRGVLTPVWIEYYPLWEVQRASGTPRTEIPDRLKKISM
                     DETIITRHSLLEWVRNERVDYVRPPRSISDKEIKPSNQSVIIDAESRVINSEETVLLT
                     YGHKSEGLEYVEDAIRELWATYDPDNQQTAPTRNDVVEYLKGRGAGQNMAEAVNLILR
                     PTGLQQVGRRKRKTNS"
     gene            complement(15488..16702)
                     /locus_tag="ACHKYO_RS00115"
                     /old_locus_tag="pgaptmp_000023"
     CDS             complement(15488..16702)
                     /locus_tag="ACHKYO_RS00115"
                     /old_locus_tag="pgaptmp_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4426934.1"
                     /translation="MLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRF
                     AGKPKMLSLGVYPEVSLSDARTARDEAKRLIAGGVNPSEVRKAQKREQANKCGNTFEA
                     IAREWYEKRLDRWSQSYAAEMIDTFEKDVFPYIGSKPIAEIKPMELLAVLSRLNDRGA
                     TEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRV
                     FNTYTGSQIVKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVP
                     LSEQAIAILEQLKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRH
                     TMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRQEMLQWYADYMDSLEQG
                     GNVVHGKFGKCG"
     gene            complement(16867..16940)
                     /locus_tag="ACHKYO_RS00120"
                     /old_locus_tag="pgaptmp_000024"
     tRNA            complement(16867..16940)
                     /locus_tag="ACHKYO_RS00120"
                     /old_locus_tag="pgaptmp_000024"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16906..16908),aa:Gly,seq:ccc)
     gene            complement(17019..17774)
                     /gene="actS"
                     /locus_tag="ACHKYO_RS00125"
                     /old_locus_tag="pgaptmp_000025"
     CDS             complement(17019..17774)
                     /gene="actS"
                     /locus_tag="ACHKYO_RS00125"
                     /old_locus_tag="pgaptmp_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HGU4426935.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            18189..20486
                     /gene="xdhA"
                     /locus_tag="ACHKYO_RS00130"
                     /old_locus_tag="pgaptmp_000026"
     CDS             18189..20486
                     /gene="xdhA"
                     /locus_tag="ACHKYO_RS00130"
                     /old_locus_tag="pgaptmp_000026"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HGU4426936.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            20497..21375
                     /gene="xdhB"
                     /locus_tag="ACHKYO_RS00135"
                     /old_locus_tag="pgaptmp_000027"
     CDS             20497..21375
                     /gene="xdhB"
                     /locus_tag="ACHKYO_RS00135"
                     /old_locus_tag="pgaptmp_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HGU4426937.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            21372..21851
                     /gene="xdhC"
                     /locus_tag="ACHKYO_RS00140"
                     /old_locus_tag="pgaptmp_000028"
     CDS             21372..21851
                     /gene="xdhC"
                     /locus_tag="ACHKYO_RS00140"
                     /old_locus_tag="pgaptmp_000028"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HGU4426938.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(21891..23669)
                     /gene="ygeV"
                     /locus_tag="ACHKYO_RS00145"
                     /old_locus_tag="pgaptmp_000029"
     CDS             complement(21891..23669)
                     /gene="ygeV"
                     /locus_tag="ACHKYO_RS00145"
                     /old_locus_tag="pgaptmp_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGU4426939.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGLGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            24148..25335
                     /gene="ygeW"
                     /locus_tag="ACHKYO_RS00150"
                     /old_locus_tag="pgaptmp_000030"
     CDS             24148..25335
                     /gene="ygeW"
                     /locus_tag="ACHKYO_RS00150"
                     /old_locus_tag="pgaptmp_000030"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HGU4426940.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            25393..26589
                     /gene="dpaL"
                     /locus_tag="ACHKYO_RS00155"
                     /old_locus_tag="pgaptmp_000031"
     CDS             25393..26589
                     /gene="dpaL"
                     /locus_tag="ACHKYO_RS00155"
                     /old_locus_tag="pgaptmp_000031"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HGU4426941.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            26647..27858
                     /gene="ygeY"
                     /locus_tag="ACHKYO_RS00160"
                     /old_locus_tag="pgaptmp_000032"
     CDS             26647..27858
                     /gene="ygeY"
                     /locus_tag="ACHKYO_RS00160"
                     /old_locus_tag="pgaptmp_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HGU4426942.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            27911..29296
                     /gene="hyuA"
                     /locus_tag="ACHKYO_RS00165"
                     /old_locus_tag="pgaptmp_000033"
     CDS             27911..29296
                     /gene="hyuA"
                     /locus_tag="ACHKYO_RS00165"
                     /old_locus_tag="pgaptmp_000033"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HGU4426943.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            29344..30276
                     /gene="arcC"
                     /locus_tag="ACHKYO_RS00170"
                     /old_locus_tag="pgaptmp_000034"
     CDS             29344..30276
                     /gene="arcC"
                     /locus_tag="ACHKYO_RS00170"
                     /old_locus_tag="pgaptmp_000034"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU4426944.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(30317..31942)
                     /gene="yqeB"
                     /locus_tag="ACHKYO_RS00175"
                     /old_locus_tag="pgaptmp_000035"
     CDS             complement(30317..31942)
                     /gene="yqeB"
                     /locus_tag="ACHKYO_RS00175"
                     /old_locus_tag="pgaptmp_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HGU4426945.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTMLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFSSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(31990..32760)
                     /gene="yqeC"
                     /locus_tag="ACHKYO_RS00180"
                     /old_locus_tag="pgaptmp_000036"
     CDS             complement(31990..32760)
                     /gene="yqeC"
                     /locus_tag="ACHKYO_RS00180"
                     /old_locus_tag="pgaptmp_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HGU4426946.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            32863..33441
                     /gene="mocA"
                     /locus_tag="ACHKYO_RS00185"
                     /old_locus_tag="pgaptmp_000037"
     CDS             32863..33441
                     /gene="mocA"
                     /locus_tag="ACHKYO_RS00185"
                     /old_locus_tag="pgaptmp_000037"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HGU4426947.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            33763..36861
                     /gene="ygfK"
                     /locus_tag="ACHKYO_RS00190"
                     /old_locus_tag="pgaptmp_000038"
     CDS             33763..36861
                     /gene="ygfK"
                     /locus_tag="ACHKYO_RS00190"
                     /old_locus_tag="pgaptmp_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HGU4426948.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            36864..38192
                     /gene="ssnA"
                     /locus_tag="ACHKYO_RS00195"
                     /old_locus_tag="pgaptmp_000039"
     CDS             36864..38192
                     /gene="ssnA"
                     /locus_tag="ACHKYO_RS00195"
                     /old_locus_tag="pgaptmp_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HGU4426949.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            38243..39022
                     /gene="ygfM"
                     /locus_tag="ACHKYO_RS00200"
                     /old_locus_tag="pgaptmp_000040"
     CDS             38243..39022
                     /gene="ygfM"
                     /locus_tag="ACHKYO_RS00200"
                     /old_locus_tag="pgaptmp_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HGU4426950.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            39019..41889
                     /gene="xdhD"
                     /locus_tag="ACHKYO_RS00205"
                     /old_locus_tag="pgaptmp_000041"
     CDS             39019..41889
                     /gene="xdhD"
                     /locus_tag="ACHKYO_RS00205"
                     /old_locus_tag="pgaptmp_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HGU4426951.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            42054..43454
                     /gene="xanQ"
                     /locus_tag="ACHKYO_RS00210"
                     /old_locus_tag="pgaptmp_000042"
     CDS             42054..43454
                     /gene="xanQ"
                     /locus_tag="ACHKYO_RS00210"
                     /old_locus_tag="pgaptmp_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HGU4426952.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            43472..44788
                     /gene="guaD"
                     /locus_tag="ACHKYO_RS00215"
                     /old_locus_tag="pgaptmp_000043"
     CDS             43472..44788
                     /gene="guaD"
                     /locus_tag="ACHKYO_RS00215"
                     /old_locus_tag="pgaptmp_000043"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HGU4426953.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            44824..46191
                     /gene="ghxQ"
                     /locus_tag="ACHKYO_RS00220"
                     /old_locus_tag="pgaptmp_000044"
                     /gene_synonym="ygfQ"
     CDS             44824..46191
                     /gene="ghxQ"
                     /locus_tag="ACHKYO_RS00220"
                     /old_locus_tag="pgaptmp_000044"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HGU4426954.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(46227..46715)
                     /gene="ygfS"
                     /locus_tag="ACHKYO_RS00225"
                     /old_locus_tag="pgaptmp_000045"
     CDS             complement(46227..46715)
                     /gene="ygfS"
                     /locus_tag="ACHKYO_RS00225"
                     /old_locus_tag="pgaptmp_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU4426955.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(46715..48634)
                     /gene="ygfT"
                     /locus_tag="ACHKYO_RS00230"
                     /old_locus_tag="pgaptmp_000046"
     CDS             complement(46715..48634)
                     /gene="ygfT"
                     /locus_tag="ACHKYO_RS00230"
                     /old_locus_tag="pgaptmp_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HGU4426956.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            49070..50518
                     /gene="uacT"
                     /locus_tag="ACHKYO_RS00235"
                     /old_locus_tag="pgaptmp_000047"
     CDS             49070..50518
                     /gene="uacT"
                     /locus_tag="ACHKYO_RS00235"
                     /old_locus_tag="pgaptmp_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HGU4426957.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            50520..50645
                     /gene="yqfG"
                     /locus_tag="ACHKYO_RS00240"
                     /old_locus_tag="pgaptmp_000048"
     CDS             50520..50645
                     /gene="yqfG"
                     /locus_tag="ACHKYO_RS00240"
                     /old_locus_tag="pgaptmp_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HGU4426958.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(50642..50713)
                     /locus_tag="ACHKYO_RS00245"
                     /old_locus_tag="pgaptmp_000049"
     CDS             complement(50642..50713)
                     /locus_tag="ACHKYO_RS00245"
                     /old_locus_tag="pgaptmp_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4426959.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            50768..51316
                     /gene="idi"
                     /locus_tag="ACHKYO_RS00250"
                     /old_locus_tag="pgaptmp_000050"
     CDS             50768..51316
                     /gene="idi"
                     /locus_tag="ACHKYO_RS00250"
                     /old_locus_tag="pgaptmp_000050"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGU4426960.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSTFAQHN"
     gene            complement(51359..52876)
                     /gene="lysS"
                     /locus_tag="ACHKYO_RS00255"
                     /old_locus_tag="pgaptmp_000051"
     CDS             complement(51359..52876)
                     /gene="lysS"
                     /locus_tag="ACHKYO_RS00255"
                     /old_locus_tag="pgaptmp_000051"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU4426961.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(52886..53984)
                     /gene="prfB"
                     /locus_tag="ACHKYO_RS00260"
                     /old_locus_tag="pgaptmp_000052"
     CDS             complement(join(52886..53908,53910..53984))
                     /gene="prfB"
                     /locus_tag="ACHKYO_RS00260"
                     /old_locus_tag="pgaptmp_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGU4426962.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(54075..55808)
                     /gene="recJ"
                     /locus_tag="ACHKYO_RS00265"
                     /old_locus_tag="pgaptmp_000053"
     CDS             complement(54075..55808)
                     /gene="recJ"
                     /locus_tag="ACHKYO_RS00265"
                     /old_locus_tag="pgaptmp_000053"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGU4426963.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(55814..56524)
                     /gene="dsbC"
                     /locus_tag="ACHKYO_RS00270"
                     /old_locus_tag="pgaptmp_000054"
     CDS             complement(55814..56524)
                     /gene="dsbC"
                     /locus_tag="ACHKYO_RS00270"
                     /old_locus_tag="pgaptmp_000054"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HGU4426964.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(56549..57445)
                     /gene="xerD"
                     /locus_tag="ACHKYO_RS00275"
                     /old_locus_tag="pgaptmp_000055"
     CDS             complement(56549..57445)
                     /gene="xerD"
                     /locus_tag="ACHKYO_RS00275"
                     /old_locus_tag="pgaptmp_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGU4426965.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            57557..58078
                     /gene="fldB"
                     /locus_tag="ACHKYO_RS00280"
                     /old_locus_tag="pgaptmp_000056"
     CDS             57557..58078
                     /gene="fldB"
                     /locus_tag="ACHKYO_RS00280"
                     /old_locus_tag="pgaptmp_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HGU4426966.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(58118..58525)
                     /gene="ygfX"
                     /locus_tag="ACHKYO_RS00285"
                     /old_locus_tag="pgaptmp_000057"
     CDS             complement(58118..58525)
                     /gene="ygfX"
                     /locus_tag="ACHKYO_RS00285"
                     /old_locus_tag="pgaptmp_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HGU4426967.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(58506..58772)
                     /gene="sdhE"
                     /locus_tag="ACHKYO_RS00290"
                     /old_locus_tag="pgaptmp_000058"
     CDS             complement(58506..58772)
                     /gene="sdhE"
                     /locus_tag="ACHKYO_RS00290"
                     /old_locus_tag="pgaptmp_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HGU4426968.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            59015..59995
                     /gene="ygfZ"
                     /locus_tag="ACHKYO_RS00295"
                     /old_locus_tag="pgaptmp_000059"
     CDS             59015..59995
                     /gene="ygfZ"
                     /locus_tag="ACHKYO_RS00295"
                     /old_locus_tag="pgaptmp_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HGU4426969.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLGLKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(60191..60850)
                     /gene="yqfA"
                     /locus_tag="ACHKYO_RS00300"
                     /old_locus_tag="pgaptmp_000060"
     CDS             complement(60191..60850)
                     /gene="yqfA"
                     /locus_tag="ACHKYO_RS00300"
                     /old_locus_tag="pgaptmp_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGU4426970.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(61014..61325)
                     /gene="yqfB"
                     /locus_tag="ACHKYO_RS00305"
                     /old_locus_tag="pgaptmp_000061"
     CDS             complement(61014..61325)
                     /gene="yqfB"
                     /locus_tag="ACHKYO_RS00305"
                     /old_locus_tag="pgaptmp_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HGU4426971.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVRLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            61370..62803
                     /gene="bglA"
                     /locus_tag="ACHKYO_RS00310"
                     /old_locus_tag="pgaptmp_000062"
     CDS             61370..62803
                     /gene="bglA"
                     /locus_tag="ACHKYO_RS00310"
                     /old_locus_tag="pgaptmp_000062"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HGU4426972.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGSWNKGGKEPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            complement(62860..63603)
                     /gene="ygfF"
                     /locus_tag="ACHKYO_RS00315"
                     /old_locus_tag="pgaptmp_000063"
     CDS             complement(62860..63603)
                     /gene="ygfF"
                     /locus_tag="ACHKYO_RS00315"
                     /old_locus_tag="pgaptmp_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4426973.1"
                     /translation="MVIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNFSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(63864..66737)
                     /gene="gcvP"
                     /locus_tag="ACHKYO_RS00320"
                     /old_locus_tag="pgaptmp_000064"
     CDS             complement(63864..66737)
                     /gene="gcvP"
                     /locus_tag="ACHKYO_RS00320"
                     /old_locus_tag="pgaptmp_000064"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HGU4426974.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(66856..67245)
                     /gene="gcvH"
                     /locus_tag="ACHKYO_RS00325"
                     /old_locus_tag="pgaptmp_000065"
     CDS             complement(66856..67245)
                     /gene="gcvH"
                     /locus_tag="ACHKYO_RS00325"
                     /old_locus_tag="pgaptmp_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HGU4426975.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(67269..68363)
                     /gene="gcvT"
                     /locus_tag="ACHKYO_RS00330"
                     /old_locus_tag="pgaptmp_000066"
     CDS             complement(67269..68363)
                     /gene="gcvT"
                     /locus_tag="ACHKYO_RS00330"
                     /old_locus_tag="pgaptmp_000066"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HGU4426976.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            68653..68736
                     /locus_tag="ACHKYO_RS00335"
                     /old_locus_tag="pgaptmp_000067"
     CDS             68653..68736
                     /locus_tag="ACHKYO_RS00335"
                     /old_locus_tag="pgaptmp_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4426977.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(68810..70012)
                     /gene="ubiI"
                     /locus_tag="ACHKYO_RS00340"
                     /old_locus_tag="pgaptmp_000068"
                     /gene_synonym="visC"
     CDS             complement(68810..70012)
                     /gene="ubiI"
                     /locus_tag="ACHKYO_RS00340"
                     /old_locus_tag="pgaptmp_000068"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HGU4426978.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHCLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(70035..71213)
                     /gene="ubiH"
                     /locus_tag="ACHKYO_RS00345"
                     /old_locus_tag="pgaptmp_000069"
                     /gene_synonym="visB"
     CDS             complement(70035..71213)
                     /gene="ubiH"
                     /locus_tag="ACHKYO_RS00345"
                     /old_locus_tag="pgaptmp_000069"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HGU4426979.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(71210..72535)
                     /gene="pepP"
                     /locus_tag="ACHKYO_RS00350"
                     /old_locus_tag="pgaptmp_000070"
     CDS             complement(71210..72535)
                     /gene="pepP"
                     /locus_tag="ACHKYO_RS00350"
                     /old_locus_tag="pgaptmp_000070"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HGU4426980.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYHGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(72561..73139)
                     /gene="ygfB"
                     /locus_tag="ACHKYO_RS00355"
                     /old_locus_tag="pgaptmp_000071"
     CDS             complement(72561..73139)
                     /gene="ygfB"
                     /locus_tag="ACHKYO_RS00355"
                     /old_locus_tag="pgaptmp_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HGU4426981.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            73307..73636
                     /gene="zapA"
                     /locus_tag="ACHKYO_RS00360"
                     /old_locus_tag="pgaptmp_000072"
     CDS             73307..73636
                     /gene="zapA"
                     /locus_tag="ACHKYO_RS00360"
                     /old_locus_tag="pgaptmp_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU4426982.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            73678..73861
                     /gene="ssrS"
                     /locus_tag="ACHKYO_RS00365"
                     /old_locus_tag="pgaptmp_000073"
     ncRNA           73678..73861
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKYO_RS00365"
                     /old_locus_tag="pgaptmp_000073"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            73882..74484
                     /gene="fau"
                     /locus_tag="ACHKYO_RS00370"
                     /old_locus_tag="pgaptmp_000074"
     CDS             73882..74484
                     /gene="fau"
                     /locus_tag="ACHKYO_RS00370"
                     /old_locus_tag="pgaptmp_000074"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGU4426983.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(74585..74644)
                     /locus_tag="ACHKYO_RS00375"
                     /old_locus_tag="pgaptmp_000075"
     CDS             complement(74585..74644)
                     /locus_tag="ACHKYO_RS00375"
                     /old_locus_tag="pgaptmp_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4426984.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(74977..75036)
                     /gene="ibsC"
                     /locus_tag="ACHKYO_RS00380"
                     /old_locus_tag="pgaptmp_000076"
     CDS             complement(74977..75036)
                     /gene="ibsC"
                     /locus_tag="ACHKYO_RS00380"
                     /old_locus_tag="pgaptmp_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HGU4426985.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(75265..76497)
                     /gene="serA"
                     /locus_tag="ACHKYO_RS00385"
                     /old_locus_tag="pgaptmp_000077"
     CDS             complement(75265..76497)
                     /gene="serA"
                     /locus_tag="ACHKYO_RS00385"
                     /old_locus_tag="pgaptmp_000077"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGU4426986.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(76753..77412)
                     /gene="rpiA"
                     /locus_tag="ACHKYO_RS00390"
                     /old_locus_tag="pgaptmp_000078"
     CDS             complement(76753..77412)
                     /gene="rpiA"
                     /locus_tag="ACHKYO_RS00390"
                     /old_locus_tag="pgaptmp_000078"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGU4426987.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(77468..77653)
                     /locus_tag="ACHKYO_RS00395"
                     /old_locus_tag="pgaptmp_000079"
     CDS             complement(77468..77653)
                     /locus_tag="ACHKYO_RS00395"
                     /old_locus_tag="pgaptmp_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4426988.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            77795..78688
                     /gene="argP"
                     /locus_tag="ACHKYO_RS00400"
                     /old_locus_tag="pgaptmp_000080"
     CDS             77795..78688
                     /gene="argP"
                     /locus_tag="ACHKYO_RS00400"
                     /old_locus_tag="pgaptmp_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HGU4426989.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            78892..81036
                     /gene="scpA"
                     /locus_tag="ACHKYO_RS00405"
                     /old_locus_tag="pgaptmp_000081"
                     /pseudo
     CDS             78892..81036
                     /gene="scpA"
                     /locus_tag="ACHKYO_RS00405"
                     /old_locus_tag="pgaptmp_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            81029..82024
                     /gene="meaB"
                     /locus_tag="ACHKYO_RS00410"
                     /old_locus_tag="pgaptmp_000082"
     CDS             81029..82024
                     /gene="meaB"
                     /locus_tag="ACHKYO_RS00410"
                     /old_locus_tag="pgaptmp_000082"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HGU4426990.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLHHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            82035..82820
                     /gene="scpB"
                     /locus_tag="ACHKYO_RS00415"
                     /old_locus_tag="pgaptmp_000083"
     CDS             82035..82820
                     /gene="scpB"
                     /locus_tag="ACHKYO_RS00415"
                     /old_locus_tag="pgaptmp_000083"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HGU4426991.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            82844..84322
                     /gene="scpC"
                     /locus_tag="ACHKYO_RS00420"
                     /old_locus_tag="pgaptmp_000084"
     CDS             82844..84322
                     /gene="scpC"
                     /locus_tag="ACHKYO_RS00420"
                     /old_locus_tag="pgaptmp_000084"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HGU4426992.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(84319..85215)
                     /gene="ygfI"
                     /locus_tag="ACHKYO_RS00425"
                     /old_locus_tag="pgaptmp_000085"
     CDS             complement(84319..85215)
                     /gene="ygfI"
                     /locus_tag="ACHKYO_RS00425"
                     /old_locus_tag="pgaptmp_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4426993.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(85382..86122)
                     /gene="yggE"
                     /locus_tag="ACHKYO_RS00430"
                     /old_locus_tag="pgaptmp_000086"
     CDS             complement(85382..86122)
                     /gene="yggE"
                     /locus_tag="ACHKYO_RS00430"
                     /old_locus_tag="pgaptmp_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HGU4426994.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(86215..86850)
                     /gene="argO"
                     /locus_tag="ACHKYO_RS00435"
                     /old_locus_tag="pgaptmp_000087"
     CDS             complement(86215..86850)
                     /gene="argO"
                     /locus_tag="ACHKYO_RS00435"
                     /old_locus_tag="pgaptmp_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HGU4426995.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKAQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(86989..87849)
                     /gene="mscS"
                     /locus_tag="ACHKYO_RS00440"
                     /old_locus_tag="pgaptmp_000088"
     CDS             complement(86989..87849)
                     /gene="mscS"
                     /locus_tag="ACHKYO_RS00440"
                     /old_locus_tag="pgaptmp_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HGU4426996.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            88045..88143
                     /locus_tag="ACHKYO_RS00445"
                     /old_locus_tag="pgaptmp_000089"
                     /pseudo
     CDS             88045..88143
                     /locus_tag="ACHKYO_RS00445"
                     /old_locus_tag="pgaptmp_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(88207..89286)
                     /gene="fbaA"
                     /locus_tag="ACHKYO_RS00450"
                     /old_locus_tag="pgaptmp_000090"
     CDS             complement(88207..89286)
                     /gene="fbaA"
                     /locus_tag="ACHKYO_RS00450"
                     /old_locus_tag="pgaptmp_000090"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGU4426997.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(89501..90664)
                     /gene="pgk"
                     /locus_tag="ACHKYO_RS00455"
                     /old_locus_tag="pgaptmp_000091"
     CDS             complement(89501..90664)
                     /gene="pgk"
                     /locus_tag="ACHKYO_RS00455"
                     /old_locus_tag="pgaptmp_000091"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGU4426998.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(90714..91733)
                     /gene="epd"
                     /locus_tag="ACHKYO_RS00460"
                     /old_locus_tag="pgaptmp_000092"
     CDS             complement(90714..91733)
                     /gene="epd"
                     /locus_tag="ACHKYO_RS00460"
                     /old_locus_tag="pgaptmp_000092"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HGU4426999.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            92105..92536
                     /locus_tag="ACHKYO_RS00465"
                     /old_locus_tag="pgaptmp_000093"
     CDS             92105..92536
                     /locus_tag="ACHKYO_RS00465"
                     /old_locus_tag="pgaptmp_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HGU4427000.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            92559..93137
                     /locus_tag="ACHKYO_RS00470"
                     /old_locus_tag="pgaptmp_000094"
     CDS             92559..93137
                     /locus_tag="ACHKYO_RS00470"
                     /old_locus_tag="pgaptmp_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGU4427001.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTELFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            93138..93845
                     /locus_tag="ACHKYO_RS00475"
                     /old_locus_tag="pgaptmp_000095"
     CDS             93138..93845
                     /locus_tag="ACHKYO_RS00475"
                     /old_locus_tag="pgaptmp_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGU4427002.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNNLAVRGAALDMRAFRIQNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            93833..94510
                     /locus_tag="ACHKYO_RS00480"
                     /old_locus_tag="pgaptmp_000096"
     CDS             93833..94510
                     /locus_tag="ACHKYO_RS00480"
                     /old_locus_tag="pgaptmp_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4427003.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            94504..95181
                     /locus_tag="ACHKYO_RS00485"
                     /old_locus_tag="pgaptmp_000097"
     CDS             94504..95181
                     /locus_tag="ACHKYO_RS00485"
                     /old_locus_tag="pgaptmp_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4427004.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(95153..95866)
                     /locus_tag="ACHKYO_RS00490"
                     /old_locus_tag="pgaptmp_000098"
     CDS             complement(95153..95866)
                     /locus_tag="ACHKYO_RS00490"
                     /old_locus_tag="pgaptmp_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HGU4427005.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(95863..96372)
                     /gene="fumE"
                     /locus_tag="ACHKYO_RS00495"
                     /old_locus_tag="pgaptmp_000099"
     CDS             complement(95863..96372)
                     /gene="fumE"
                     /locus_tag="ACHKYO_RS00495"
                     /old_locus_tag="pgaptmp_000099"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HGU4427006.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(96394..97359)
                     /gene="glpX"
                     /locus_tag="ACHKYO_RS00500"
                     /old_locus_tag="pgaptmp_000100"
     CDS             complement(96394..97359)
                     /gene="glpX"
                     /locus_tag="ACHKYO_RS00500"
                     /old_locus_tag="pgaptmp_000100"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU4427007.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(97356..98633)
                     /gene="yggP"
                     /locus_tag="ACHKYO_RS00505"
                     /old_locus_tag="pgaptmp_000101"
     CDS             complement(97356..98633)
                     /gene="yggP"
                     /locus_tag="ACHKYO_RS00505"
                     /old_locus_tag="pgaptmp_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU4427008.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(98648..100036)
                     /gene="cmtA"
                     /locus_tag="ACHKYO_RS00510"
                     /old_locus_tag="pgaptmp_000102"
     CDS             complement(98648..100036)
                     /gene="cmtA"
                     /locus_tag="ACHKYO_RS00510"
                     /old_locus_tag="pgaptmp_000102"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HGU4427009.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(100064..100507)
                     /gene="cmtB"
                     /locus_tag="ACHKYO_RS00515"
                     /old_locus_tag="pgaptmp_000103"
     CDS             complement(100064..100507)
                     /gene="cmtB"
                     /locus_tag="ACHKYO_RS00515"
                     /old_locus_tag="pgaptmp_000103"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HGU4427010.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(100821..102812)
                     /gene="tkt"
                     /locus_tag="ACHKYO_RS00520"
                     /old_locus_tag="pgaptmp_000104"
     CDS             complement(100821..102812)
                     /gene="tkt"
                     /locus_tag="ACHKYO_RS00520"
                     /old_locus_tag="pgaptmp_000104"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU4427011.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            103090..103848
                     /gene="loiP"
                     /locus_tag="ACHKYO_RS00525"
                     /old_locus_tag="pgaptmp_000105"
                     /gene_synonym="yggG"
     CDS             103090..103848
                     /gene="loiP"
                     /locus_tag="ACHKYO_RS00525"
                     /old_locus_tag="pgaptmp_000105"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HGU4427012.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(103904..104647)
                     /locus_tag="ACHKYO_RS00530"
                     /old_locus_tag="pgaptmp_000106"
     CDS             complement(103904..104647)
                     /locus_tag="ACHKYO_RS00530"
                     /old_locus_tag="pgaptmp_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGU4427013.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(104634..105743)
                     /gene="dgaE"
                     /locus_tag="ACHKYO_RS00535"
                     /old_locus_tag="pgaptmp_000107"
     CDS             complement(104634..105743)
                     /gene="dgaE"
                     /locus_tag="ACHKYO_RS00535"
                     /old_locus_tag="pgaptmp_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HGU4427014.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(105747..106607)
                     /locus_tag="ACHKYO_RS00540"
                     /old_locus_tag="pgaptmp_000108"
     CDS             complement(105747..106607)
                     /locus_tag="ACHKYO_RS00540"
                     /old_locus_tag="pgaptmp_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGU4427015.1"
                     /translation="MMSDDVMQHELVERARESGTLTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(106604..107353)
                     /locus_tag="ACHKYO_RS00545"
                     /old_locus_tag="pgaptmp_000109"
     CDS             complement(106604..107353)
                     /locus_tag="ACHKYO_RS00545"
                     /old_locus_tag="pgaptmp_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGU4427016.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(107379..107864)
                     /locus_tag="ACHKYO_RS00550"
                     /old_locus_tag="pgaptmp_000110"
     CDS             complement(107379..107864)
                     /locus_tag="ACHKYO_RS00550"
                     /old_locus_tag="pgaptmp_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGU4427017.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(107875..108303)
                     /locus_tag="ACHKYO_RS00555"
                     /old_locus_tag="pgaptmp_000111"
     CDS             complement(107875..108303)
                     /locus_tag="ACHKYO_RS00555"
                     /old_locus_tag="pgaptmp_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU4427018.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(108422..111220)
                     /gene="dagR"
                     /locus_tag="ACHKYO_RS00560"
                     /old_locus_tag="pgaptmp_000112"
     CDS             complement(108422..111220)
                     /gene="dagR"
                     /locus_tag="ACHKYO_RS00560"
                     /old_locus_tag="pgaptmp_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HGU4427019.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(111479..112399)
                     /gene="speB"
                     /locus_tag="ACHKYO_RS00565"
                     /old_locus_tag="pgaptmp_000113"
     CDS             complement(111479..112399)
                     /gene="speB"
                     /locus_tag="ACHKYO_RS00565"
                     /old_locus_tag="pgaptmp_000113"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HGU4427020.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(112535..113266)
                     /locus_tag="ACHKYO_RS00570"
                     /old_locus_tag="pgaptmp_000114"
     CDS             complement(112535..113266)
                     /locus_tag="ACHKYO_RS00570"
                     /old_locus_tag="pgaptmp_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4427021.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(113412..115388)
                     /gene="speA"
                     /locus_tag="ACHKYO_RS00575"
                     /old_locus_tag="pgaptmp_000115"
     CDS             complement(113412..115388)
                     /gene="speA"
                     /locus_tag="ACHKYO_RS00575"
                     /old_locus_tag="pgaptmp_000115"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HGU4427022.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(115397..115528)
                     /gene="yqgB"
                     /locus_tag="ACHKYO_RS00580"
                     /old_locus_tag="pgaptmp_000116"
     CDS             complement(115397..115528)
                     /gene="yqgB"
                     /locus_tag="ACHKYO_RS00580"
                     /old_locus_tag="pgaptmp_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HGU4427023.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(115622..115765)
                     /gene="yqgG"
                     /locus_tag="ACHKYO_RS00585"
                     /old_locus_tag="pgaptmp_000117"
     CDS             complement(115622..115765)
                     /gene="yqgG"
                     /locus_tag="ACHKYO_RS00585"
                     /old_locus_tag="pgaptmp_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HGU4427024.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(115876..116127)
                     /gene="yqgD"
                     /locus_tag="ACHKYO_RS00590"
                     /old_locus_tag="pgaptmp_000118"
     CDS             complement(115876..116127)
                     /gene="yqgD"
                     /locus_tag="ACHKYO_RS00590"
                     /old_locus_tag="pgaptmp_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HGU4427025.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            116183..117337
                     /gene="metK"
                     /locus_tag="ACHKYO_RS00595"
                     /old_locus_tag="pgaptmp_000119"
     CDS             116183..117337
                     /gene="metK"
                     /locus_tag="ACHKYO_RS00595"
                     /old_locus_tag="pgaptmp_000119"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGU4427026.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            117773..119167
                     /gene="galP"
                     /locus_tag="ACHKYO_RS00600"
                     /old_locus_tag="pgaptmp_000120"
     CDS             117773..119167
                     /gene="galP"
                     /locus_tag="ACHKYO_RS00600"
                     /old_locus_tag="pgaptmp_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HGU4427027.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            119244..119741
                     /gene="yggI"
                     /locus_tag="ACHKYO_RS00605"
                     /old_locus_tag="pgaptmp_000121"
     CDS             119244..119741
                     /gene="yggI"
                     /locus_tag="ACHKYO_RS00605"
                     /old_locus_tag="pgaptmp_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HGU4427028.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            119836..120543
                     /gene="endA"
                     /locus_tag="ACHKYO_RS00610"
                     /old_locus_tag="pgaptmp_000122"
     CDS             119836..120543
                     /gene="endA"
                     /locus_tag="ACHKYO_RS00610"
                     /old_locus_tag="pgaptmp_000122"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HGU4427029.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            120623..121354
                     /gene="rsmE"
                     /locus_tag="ACHKYO_RS00615"
                     /old_locus_tag="pgaptmp_000123"
     CDS             120623..121354
                     /gene="rsmE"
                     /locus_tag="ACHKYO_RS00615"
                     /old_locus_tag="pgaptmp_000123"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGU4427030.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            121367..122317
                     /gene="gshB"
                     /locus_tag="ACHKYO_RS00620"
                     /old_locus_tag="pgaptmp_000124"
     CDS             121367..122317
                     /gene="gshB"
                     /locus_tag="ACHKYO_RS00620"
                     /old_locus_tag="pgaptmp_000124"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HGU4427031.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            122426..122989
                     /gene="yqgE"
                     /locus_tag="ACHKYO_RS00625"
                     /old_locus_tag="pgaptmp_000125"
     CDS             122426..122989
                     /gene="yqgE"
                     /locus_tag="ACHKYO_RS00625"
                     /old_locus_tag="pgaptmp_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HGU4427032.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            122989..123405
                     /gene="ruvX"
                     /locus_tag="ACHKYO_RS00630"
                     /old_locus_tag="pgaptmp_000126"
     CDS             122989..123405
                     /gene="ruvX"
                     /locus_tag="ACHKYO_RS00630"
                     /old_locus_tag="pgaptmp_000126"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGU4427033.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
BASE COUNT        29922 a        32805 c        30414 g        30402 t
ORIGIN      
        1 gttggacatc tctttcaaca tgagagtcat tgaagctttc gaccaattta gcggaatgat
       61 agggcttggc gcttcatgca aagttcccga cttgtggata tagttatagc tttcgggaag
      121 tgccatttca ggtacactcc tgccatttgc accaatcata ttctcgcctg ttatttgggc
      181 caatgatcta ttcataaatt gcactgagtc attcaaatac tcttcctttc ttgattcttt
      241 ataaacaatt gctgctgatt ttgcatacca tgagtcaaaa aaccattctg cttctgttga
      301 tggaatgaag ctcttttctc ttttcgcatg tgaattttga tcggtatcgg ttttaatatc
      361 attgaaccaa aaatttgccg actgataatt atccttctcg tatcttttta taccatacgg
      421 acctgctagt tttttaacta tttttagaac ctgctctttt cttctggtgt ttatctttgc
      481 aagatttgca ggataaataa cattaagtaa agccgcatcg gcttctctgt aatgtttatc
      541 tttttccaga taacctgggg attctccccc taaatcaagc tgtaacgtta ttctttcata
      601 acctttatct atgaggtggt tcaacctcgt tgttgaaaga ggttcatcca gttcattggc
      661 tttagcttcg cgtagtaaat cagagacaaa aacagagtct ttcttcgaga gtaagttcga
      721 caaacgttcc agtccagagg tcaccagtgc aacggaagat gtgtttaatc gggcatcttc
      781 ttcccatgct ccggaatctt ccataacata gaaattggct ttatccagat aagcaatcag
      841 cagcgcaacc gattttaatc ggtcaccttt ttgccaatct tcagcgttaa ttgtatcagt
      901 gtcgattgct tgtataagga gatcaagata aagacctaac gcatcatttt gtttatggtt
      961 ccacagctgc ggcttgcctt cttcctgcac atctgccatc acgggggagt tgctgtcaaa
     1021 tcggatatgc acaacattca tttgcccggg aactccatcc agtcgtttgg ggttgctaat
     1081 gacgtcctgc atccgcttaa tctggtcggg cgtagacata taatcccaca gggtgagcag
     1141 aacctttttt gcggctacac tatttccctg atctgatact aatgccatat agccccataa
     1201 actatccctc agccagacag catcatagtg ggtttctgcc gctgttttgt catcttcgaa
     1261 agtagaagca agaataaacg ttccccaggg tttctccgtc aacgttaact ttcgtgtgtt
     1321 ttctgttaat gttagcaatc cttgaatttc gtctttggta tagtggctct ttatttcatt
     1381 ttgtaatttg gctgagtgaa tcaaaagacg ataattagga tacttatctg gatttaacga
     1441 taaataactt ttatctgttg ctgcccccgt ggaattaaga atgttaaata cagattcact
     1501 cgtcacttct gcccctgtcg cctggcatgt gccgcacgaa aatataccag cgagcagaag
     1561 agttaatgca gatttccctt tcataaaata ttccatcaat cgtttgttaa aagcgtagat
     1621 aaccacttat ttaacaactt gaaaataagg tgttttacct gggttgttac aaaaggattg
     1681 cattgcgtaa acgcttttta tttacaacaa aatggggaag tattacggcg attgcgaaaa
     1741 gtctgaagcg atgaattaag ataatataga aaatcagata cagcaaaaat ggcagattga
     1801 tcaggtgaag ataaattgga gcggcgaagg gaacagaaca acaagccatg ccaccagtaa
     1861 taaagccatt tccacatgtt ctaaaatcaa tcctatacac atttctatac acatttagca
     1921 ggcgcttgat gacttactca ggcaaatatt taccggaatt tttccggtta tcccctatga
     1981 agctggtggg ctttacctgt ttttatccga ctgtgtcaca taacttttgt tccggcatga
     2041 taaaatcgtc tggtcatgtc cattactcat tgttgacctc ttgttgtgtt tctttggtgg
     2101 catgccgtag tgttatcgca ctacggtttt tttccctcaa actgaaatgg attttttccg
     2161 gtaatcacac ttccgcctga ttcatctgtg tacccaatgt gtggtaagca cctcatacag
     2221 caaacgaggc aatcggtatt ctgctccttc atggcgtttt tagcgctgtg gtgaataaca
     2281 gaatgacgga acgatagcct atggatactc acgacaagat accaccagca tattcatact
     2341 ccccgcaacc agtaccggaa gcctggaaaa tgcgattcag cgctaacagt gcgtggttct
     2401 tcaccgatga tttaaccgtg gttgatgcct ggctaaaggc ccacagcgac ggcgtggagg
     2461 ttgtaccgct gttttgtggc tctgacagtg acggtaagcg ggataccgca tttgaggagg
     2521 tgtgcgcggc tcttgcccgt atgctgcata tgcaccgctt gttacttgag aaaacaagat
     2581 accaggaaca tgttactgac cctgaatgtc tgcgggagct ccacgaagcc ccaatacagg
     2641 cgctaaatat tctggttaaa cacaaactgt cctgaccgtg tcatggaggc ctgcactttt
     2701 tgtccaggtc tcccgattcc catccggtcc tccgcccatc gaattttaag ccgcctgtaa
     2761 gcctgtatga cgttttccgg tgaatgctcg ccccgatagt gaacattgcg ccagagtgcg
     2821 atttatcccc attgctgtgg atgtttgagc gtgagcgctg gtaactggtt aatgaggtcg
     2881 tgcctgtgcg cttgcactta acgggcaacc gtgggagatg tggggggatt ttacgaaatt
     2941 ttcgtaattt ccctttcctt gttccggcgg ggctaattct cgcgattcgc gataattaag
     3001 tttaatcaat ggcctgtagt gattatttaa tacattgtgc tggggagggt tacgaaattt
     3061 tcgtaagcca gccttccttg ttccggtggg ggaaggtgtg gtcattatga ccatccctac
     3121 ggtaatgatg attcacgctt tgctaaaggt attacgaaaa tcataatacc ttctgctggt
     3181 ggtgtccggc ttgttcgtgg ctaatcactc cctccatacc tggctttttc cggttaacct
     3241 ttaatcaggc tggtgggctt tacatgccct tgatcaccat aatgatgatt caggtatgca
     3301 ccttttcccc caacggggta aaagctccaa ctgtaacgat gttcgttatg gttgcctcga
     3361 tggtctgagt ggtcattatg accattcagg gtgcaccgtc ctttgtctcg tggcataatt
     3421 atcaggcgat tacgtttttc atgattgatt ggcaagccgt atttctccac cgcagtttcc
     3481 tcatgactgc ggttttttta tgccgttatg ccgtcactga atcgcgctca acataccgat
     3541 ccatctccag cttcacatcc agcatcatca acatgccttc aacaaccccc tccgctttgt
     3601 gaaggcgttt acctatacag gtgtcagagc acccatgttt ccgtgccagc gccatgaacg
     3661 tctcccccaa cacgtaataa tcaaccagca agtcatgcag atcgcgattg ttccggtaaa
     3721 gacgggctat gcacccgcat atcaccatcg catcatcgtc acagcactgt gagcgtgatg
     3781 ttatctttgc cggaataatc cccttaaatc ctgcggcaac ggatgaccag gtcacattct
     3841 cgcgattatt tgccgcccac gctccccagc gttccagaac ctgctgaata tcacgcatca
     3901 acaatcacct cgtaatagct gtggttacat acccgtaatt cagggatgca ctggatgaaa
     3961 gttaaactac gaaaatttcg tagcttgtga ctgtctttca gccagcttgt taacagtcct
     4021 cagaagtgag gaatgtagcc agcgcaattt tgcgtttgcg gtctcctcaa attgaggttt
     4081 ccgaaaatat cagtaggtta ggccaaagct caaaaatgag ctttgtaaca atatcagcca
     4141 gttaacgccg tcagctaccc ggctcagaat tgagccgcct tttaatcaac aagttaccgc
     4201 cgtaaccgct ccggcttatg ccactggtac gggggcgcgt tctggcgttg ctcctgccgt
     4261 ctcctgacca ccatatgttc caccgtgtac agcgtgctta gaaaaaaccc cagattcgtg
     4321 gtttttctcc ggctcttttt gcgactcgtt taaaaccagt cacagagtcg cttaaagcta
     4381 ctcagttcga caccatcacg tcacatcagc ctgattttca gcgctcacgc tgtccggctg
     4441 cgtggcctcc tgtttgcgct ggcgttctct ctgctcttca atcatgagga ttaactcgcg
     4501 ttcctcctgc tcttcaataa tcccgtgaat tatctctgtt ggtgaacgtt cggtcatcgg
     4561 ccctcctgtg tgtcactgtt ttggtgtcaa tgtcactgtt acggcgacac cgtattttgc
     4621 ccaaatcagg caaaattgct tacccgaacc cggtaaattt gcgcgctttt tcgatcatcg
     4681 cgtatcggtt tcgttttgca cttcctgttg cgtttcgttt ttgagaaaaa tgcgcacttt
     4741 ttccggtggc ttttccccct acaagccacg tcatatctgg ctttgctctg ctggtctgtg
     4801 tgatcgattt tgtgatcggt tttatgatcg atttgcgcag ttttttgcgc attttttcag
     4861 ggatttttca ccccatcgaa gaggcttcta aggggctgtg aagtgggtag cattttgctg
     4921 tgatggaagg actaccgcta ctgtctgata atggtgacgg aggccgctat gttcggctct
     4981 gattgcagcc tgccgtatat tcaccaattt acctggctgt tatttccata acccgtaatg
     5041 tcgccgatcc cgtctgattc ccatatcgtc aacagtctta tttacagcat cctgaagttc
     5101 gcaaggcata tttcccgccg tctggctata tgctctcgct gcggatcggt gtgggcctct
     5161 ttcattacgt caagccaacg agcggcggct cttcgccata gtccctgaga ttccagttgt
     5221 tcggcttttt tatcagttat tgtgtccata ttaacttccg tctcttcgcc ctgtttcacc
     5281 cctgtacttc gtgccccgta aaggatcttc ttttttctgg tcaatcccca tatctttaag
     5341 cgttctggtc gctgctgccc tgacggcgca tacatgcccc cactctgttg tttttggtct
     5401 ggtggctttt gccgtacatt gtgcccttcg ttgggtaata tagtgcctct cttcatcact
     5461 ttttgcctgt ctgaacaatt ctccccagcg ttcagcagca cgccgccaga ggccgctatt
     5521 ctccagcgct tcagccttgt catcacgtat catctgtacc ccgccgattc tgtcctttca
     5581 aaatttgcct tatggcttat tgtatgaggg attcatccta caacaagaaa ggtcactatg
     5641 agactgacgc aatacaaaaa agagatcctc agttacttca ctcctgacaa tctgtcgtgg
     5701 gtcagaggtg aaattggtaa cccgccgttt gatgtctccg gtgtggctta cctgctgtat
     5761 ggcatagatt cacacaacaa ccgatatcag ctcgaatcaa caaggcgcac acttgaagcg
     5821 atggtccgtg acggtcttct ggagcgggtt aacgtgtatg agcggcgtga gggaacaggt
     5881 gatggtgttc gtcgaaaggt ggcgcgttat ggcctgcctg gtcattctgt tgtggtgcgt
     5941 gacggtgagg gccggaacgg tgcaattgac ggcgaatgta tacgcatcaa tgctacagat
     6001 gtggtgctgg tggacgggca ataaaaaacc ctgaataact ccgctgggtg cttcaattgc
     6061 acggggcgaa atccttcagg gtcagccgta acgggtctct gtacaggtgg cttcagttgc
     6121 cagaagatga acgcccgtta cagcctgctg tgataagccg ctttacagtg agcttcaatt
     6181 gctcaaagag gcgcgttttt atcacaactt tgccgcaata acccgctgta cagagggctc
     6241 cagttgccca aagaggcgca ttcttatcgc ggcatgacga aatcaccgtg atgttttcgt
     6301 cgttcactca tcccgatgtg tgtgacaagt acacatcaaa atgagtgccg aaaagccgct
     6361 ggctggcttc attcggcgag gctcccgttg cctgcggaga acaccatcaa cggcctttcg
     6421 gtggtgatta tagcgaatcg cgataattga gcaaatacgg cgatgagata cgtatcacga
     6481 tagcgctaaa tcgtcgcctg catcactgaa gctgtaattt ttccattcat cctgcggatt
     6541 aatccccatc tctttcagcg ttctgtctgc ggcctttttc acatcgccaa aggtgacgaa
     6601 ctgctcaggc atacgacggg atttaatgat gcatatcgcc cgacgtctgg cagcttcctc
     6661 gcgctccctg tccgtttctg cgtgaagaag gacgtcagag catcgggtcg ccgcacgtcg
     6721 ccataaacca cgctcctcaa gtttctgtgc cacgctgtct gttatcactc tcaccacctc
     6781 ccgtatcctg ccactggatg ttttgctgtt ttatctgctc cagcctccac atcagttgtt
     6841 cgctggctgc tgctttctct ttctccgtga cttcgccgca aggttgcccg ttcaggtcgt
     6901 accgtgggcc accagcaata atcgccttaa ggtatcgcgt cgtgtgaagg tgtgaggtta
     6961 atgcgccgcg taatacacct gtagcaagtg gcaactcccg tgatttagcg tcctcaagca
     7021 catcacgtat tatgccagcc ttcaggggtt ttatattctc tgtcccaaac agctcaggcc
     7081 agtattccgc aattcgattt attcgtctgt ggttttttcg ttgctctttt gtcatgtgct
     7141 gccacggtat gcgccgtgat ggtttacgtg tggtctggtc ctgttgtgtg gtctgctggt
     7201 tcatgttgtg ctcctgtcat tcagaacgga atatcgtcag agaacgggtc atcacctccc
     7261 gctggtggct gatttccctg ctgtccggtc tgtcgttttg ctctttccag tgcttcggtc
     7321 gccttcccct gttgcccccg cttcccgcca ggacgcgccg aacgggcact gattacgctg
     7381 tcagcgataa cctgccagcc ctgccgtatt tctccgttct gtccggtcca ctggctgatc
     7441 tgcatgttac ccgccacgct gataaggtcg cctttttcat gtctcgccag cgcgtcggct
     7501 tgcttaccaa atgccaggac tgatagccat aacgtcgcct gtccgtcctg tgcctgactg
     7561 catggcaaag ataccgccat acgcgccagt gccattggtg ttcctttgct ggtctgtttt
     7621 tcctgcgggt cgtccaccag ccgcccgtaa gctgcaatct gtgctgtcat gattccacct
     7681 ctccggtttt aacgttgatg gttgctacct gttccgcttc agcaatctcc cgttctgtca
     7741 gcgtggcaaa gtttgccgcc gccgtggtca tgaatgcgct tatcagttcg ggatgttcct
     7801 tcgcgtatcc ttctcccgtg ttgcggtcaa tgatttttat cgccaccctc agccagtgct
     7861 ctgtaatgtc aatggcgcgg tgatctgtct ttttgtggct tactttcatg atttactcca
     7921 gagtaccgct attgctgccg tcgttaaccg gataccaggc aaatacccat gactcaccgc
     7981 agtgaacaaa cttcttatct ggcagataaa ggcactcgca cgccccacaa tcagaataat
     8041 gcggctccat gcctgcttca cctggcaaga cgggtagtat ctcctgatac gcacatcccg
     8101 tattacaggg ggcgacctca caaagcgccc atgcagcaat tgggctatac cattccgtac
     8161 cgtcgtcatc tttgaacctc gcccaccagc caggaagggc gcctgatatg ctcagcgttt
     8221 taatgtcaga gttcataaat tcacctttaa gtatctttgt cctgccgtgc tgcctgattt
     8281 tcattttttc tttgctcatt acatgattct ctctgtagtt actgcgggga aatggcatta
     8341 atctcccgct ttgctctctg ttttttaact tcacatattc acttataaag acactttttg
     8401 ggcggataag cggatatgaa gaaaaatatt gtttgcattc attgtgttac gtgatttttc
     8461 gtatccaccc atatccgcct tgtatgtttt ttctcttcag aatcaaacag ataatggcgg
     8521 atacgacttt gtgtgttttt tgcacaagat tgatatttat caatttcctc tcataacagg
     8581 gcggatatat gcggataagg gtggatacgg aaatacaata taatgctctg atatataagc
     8641 actttattaa ccatatccgc ttatccgccc acttttttac gcgtacacga gaatacgatt
     8701 tttactcctc cgactggtca agaagcacaa taaagcgttg ctgtattccg ttaaccttta
     8761 tcgttttggt cgtccagttt ttatcacctg catgaatcag aattccggct tctttcagtg
     8821 cctggcatcc aaaatttcgg ttaatgccac acaggatttc ttcatcaaat actgacggta
     8881 tgatgtggta ttccggtaat ccgtcatcac gtcgccctct caccagatac cccgccagac
     8941 tggtgattct cgctccgtaa ttcacgtcca tatctccgtt ctgtctgccg tatgtgtacg
     9001 gctgaaaacg gctaagggcg tgacgctgaa tgaaatcgcg ggcgcgactg ataacctgat
     9061 atttttccct gttatccagt ccaaaatcct gcaaccagtc atcaaaagcg cgctgtgtgg
     9121 ctgcatggct ttctcccgtt gtccatccgg ttattcccgt tgccagttct ccggctgcat
     9181 ccagcattgc aaaacgggat gccacgcgtt tcacctgggg ggaagcattt tcaggaagac
     9241 gactaagcca gatgttttct ttttcgttaa ccacactgac cgcaagttct ttgttggctg
     9301 acaaccagcc gatccactca cgacccgctg ctccgcaata attcgatgca aaccgtttta
     9361 ttgctctggc gtgctgatca ccatcgtcat atccgttaaa aaccgttgta tcaataaacg
     9421 gcacacttaa caggcgcacc agttgaccag ctttaggcgt cacaccgccc ttaatcagga
     9481 aggtttcgaa atcctcctct cccgtgctca gggccgcgat tctccagcgt atcaccgcac
     9541 ggttaccgcc atccttacgc ccctgaatac gccccacacc gttaaacagg ctgtaggctg
     9601 actgcgcaat atcgttaatc cgtccgccct gaccgatttc atcaatgggg ataaagccat
     9661 cattacgtgc cgcagcttcc accgtgaggc cgtatctggt tgcatcccat gaaagtttca
     9721 gcacttcagg atcaccgtaa agacttgttg ctgcttccac tgtcgtggtt tttcctgccg
     9781 acgactgcgc aaaaagatga acaccaaaac agccgccacc aacaagggag ttcagggggg
     9841 cggcaagccc gaccagcact ccaagcatca ttgagtgatt accgcgcatc agtgaggcaa
     9901 cattgtcgcg ccactcatca gccgtacccc gaaccacata acctttaatc gccgccgaac
     9961 caccacagaa ggcaacgggc ctttctggta cgccaataat ctcaccatcc ggcatgacat
    10021 aagcgccaca atgccacccc gccgttgagg tgacctccca ctgtgtccgc tctccttcac
    10081 gctgaaggta ttccgcaagt ttgtttaaaa gtttctgact ggttgtcact gccaccccac
    10141 gcccacgcaa tcgcgaccat ccagaaggga ggccgatttc attgcggggc atggcctcat
    10201 aaataatttt ttccattccc tctggttcaa gttcgattat cagataccca tcgcagccat
    10261 catggccgat ccccacggtt cgcatacggt caccaatcca ctcacacggg cggataattt
    10321 caccggattg cttatcaacc tttggcgtaa cccagtaaac gccatcagga cggcattcaa
    10381 cgcggggttt taattcatcc tctgattgtt ctgatgtttt tttctttaca ggcagctcaa
    10441 taaccagtcc gtcagaaatg tttttgcgct cagtctccag atagttacgc cagttctgcc
    10501 gctcgttatg gtgcatacct tcggggtaat agtctgcgtc ctgtacgcct gccgccgcca
    10561 gcttctgacc tatgcctttg accatcacct gatcaaggtg tccggccttg tatatccgcg
    10621 ctgatttgcg cccgtcgggc acaatttgca gattgccaat atctttaagt tgctcatcag
    10681 ccagcacgac aggcggtgtt ttatctccgg caagttttcc gtcctgctcc tgccactgtt
    10741 tcgcgtgcgc ccacgcatca ctgccagcga aaataatcac ttccgtatcg gcgtgttttt
    10801 tccctcgagg ctgttttttt aagttcggtg cgcttttcat ttcttacctc tgctgatcat
    10861 cgtcatgtgg ctaccgtatt cattgcggta aatatccccg cacataaatt cggggtgagt
    10921 tttgctactg gcagttaagc cagtattttt tgatgctatt gaattaatct cctgaaggag
    10981 tcgtcttaat atttatttcc cgtttatttt tagccgttat tgttcgccgc gtttttcttc
    11041 ctccgtcatt aaccacaccg ccacattgct ggaaagttgt ttgataagtc ctgcaataac
    11101 agaaaggtca tggtcgtcta tttcttcgtg gttgcgttca atcatgcggc taattacttc
    11161 gacctgttgc gcttgcagtg aggcttctgc cagtgttatt tgctgcatta ctgcaccccc
    11221 ttaatttcat gatattcaga acaacaaagg attcagtcat tgacgcatat tgcatcatca
    11281 cccgatgcag caatcagcca gctacctgcc ttacctgtaa gctgcttaat cagggcgagc
    11341 aatgactcaa tctcaccctc agtcatttca cctgtgttac ccatcatcag agaacaaata
    11401 agttctgcct gcattgcgct ttttgctgct tcatcaacag tcatatcgga aaaatcacac
    11461 atgaattact cctcatcctg aatacatgtc actgcgtgga taattacgcc ggataaaccg
    11521 gctttagctg atttgttaat tactgtgctg attgcagcct ggacgttttc ggccttcacg
    11581 ttctgcgtga tgccaacggt atagccgcgc ttgttaacag cacgggaaaa gacgcggaag
    11641 gttttaagca tgagtcacct cctgacggat acgggcgaca aaaatcagat gtgcgtgcgg
    11701 atacagccat gtgaaaggct cctatctgtt gattaaggag tctcgctact acgctgctaa
    11761 acatggtggc gagacgtaac ggggttagca gactggccag ataggaacca gcgagcgcaa
    11821 aggctccccc gttacgcccc gccataatgc gggtatgggt aggcttacgg acacaaaaaa
    11881 accgcatatc ggaatgtaag cggctgtccg ctatctgatt caggctgcta aacccggtcg
    11941 ccatgtgggc gacttcggca tcatacagcc cgaaagaaga ggcgatcaac atgtttttat
    12001 ccatgtgatg cctcctgacg gatacgggcg acaaaaatca gatgtgcgtg cggcagccgg
    12061 gcgcgtgctt cgcgctcagt ggcagcggta acggtgaata ttgcaatgct gcctgtcagg
    12121 cactgcataa agcgccaggt gaatttattc aggttgtgcg agtcccgccc ttgcgggtgt
    12181 gtgatatgat ttctcatagc tgcctcgata ctcttgctat cgttggtggt cagaggctgc
    12241 gaaaggacgg caatcctttt cagcctcgtt tgcattgagt gataaacact cagctggatt
    12301 ccagtataat cactaagtgg aatccacttc aagcgttttg ttttggtctt tttcgtgtat
    12361 actggattcc agtaatcaca aagggaagca gaaatggaaa gagaccatat caataacaaa
    12421 tcacagaaat tacaggctcg cgccccgcat gaagttgttg aggctatgga gcaagtaaaa
    12481 gaaacaggcg agagcactgc gcaatttatc gttacagcca tgcgaggcga gatcaaacgc
    12541 cgccagcgcc gcaaggccaa agaatcagaa taatcactat cagcgccgtg gtgtgaggta
    12601 ctacggcgca ttgctttaca gggcagcagc atgaacaaaa agtaccaatc cattacagat
    12661 atttttgccg aacaacgaag acagcttttg caccgcatac ttgagcgcca actggaactg
    12721 aaacgcgcta aagagatatc agaacccgaa gagttaacgc ctcttcccgg agtgaaaaaa
    12781 cacccacaaa aacgatttgc ccttgaagat tttgtcgaga aatacccacg gcggttaaaa
    12841 gccactaaaa accgaccgcc tggctgatag ccttgtccac cagctacact catggcatag
    12901 ttggtgatgc gcttgatttc ctttatctcc actggcgacc ctttcgtggt cgcctttgtt
    12961 ttgcctgcca ttcctgcatc atgcggatat tcacgccgta cagggccacc agcgcgataa
    13021 tttgataaag cctccttctg ataatgctta acgtgtttca tgcgctcagc ggcgaaatgg
    13081 ctcacgatat ggcgcatgta atcagcatgg ttgatgctca cctgtgcgaa cttatccgca
    13141 gtatcgatgt acagggcggc agcgttgcgg atgtcaggca tgttcacgtt ctcccaggcg
    13201 acgtgccagc cagaatcaaa ctgttcaata tttacacttt ggctcccccg tttcggggaa
    13261 tcaaagtaca aattttctac agcctcaaaa tccacacctt ttttaaatcc gtattcctca
    13321 atacgccctt taatccaggt ggtgaaatca cggcctacac cgagcgcttt atgcagtgcg
    13381 cgtgcgctga caacgcccgt ttcatatctg ccaataatgc caggtgatac cggaataatt
    13441 gcggcgaaat cataattacg catgattcac ccccagagac ggcgctgacg tcatcaggct
    13501 ggcgaacgtc tccatgcggc gagcaacttc ccgcaccgct tcggtcggtg tcatgccgtt
    13561 ctgtatcgcc tcgttaaatg ctgcctgctc gattttctgc atgactatca gtaagcctaa
    13621 ctgttgctct cccgcgctct gacgctcgcc tacagccagc ccagcgaagc gattttcagt
    13681 gcgggcaagg ttgatgtaat ggtgacgttc tgcgcccagt gctttaagcc cgtcacggct
    13741 ttcgtgatag cctggcagat attcgcgacg gactggttca gcggcgcgat gcttcgcgaa
    13801 tgccttcgta acatccagct tgaactggtt gatacgttct gcgtcacgcc cacggataac
    13861 gcgtaacaga tagtcaagct gtagctcatt cagcaaataa ctaatttcag gacgaccgcg
    13921 cttgcggcca gattttcccg atttcaaata ggttaattcc ttgactgact ggcgaggcag
    13981 tgagccaagc tcgcgcaatc tggattgatg agatttaatc gtcgcaatga tactttcgtg
    14041 ttgtattcct gctcgctcag aaataacgcg ggaatcaata cgcgactctg ctttgtggcg
    14101 aatcaatata tcgttctggc tgatttcagg ctgaatgaag ccctgcccct gtacgggcat
    14161 attcggacgt ttcattgaaa actcctgctg tcggatcagg tttagtgctg ggggagaata
    14221 cccccctccc aaaagattat tacgcggcgt tgttgcgaga agcatcaata cgctggttaa
    14281 tccattcgtc gacttcactt tcaataaaag ccacggagcg ggaaccaatt tttacaggtt
    14341 tagggaagcg ccccaggctg atcaggcgat aaatccaagc gcgaccgtaa ccagtacggc
    14401 gcataacatc agaaagacga agtaagttat ttgacatatt caatcctcgt aacgtctata
    14461 accgtctaca atgatgcata aaaggaaaac atatttatag gtgggtacta tttttaatgg
    14521 tgggtactat ttcaatggaa tagtgcccac tattatttta ataatgctgc caccattcca
    14581 gtatcaataa gcaaagaaaa cgcacgctta actatgattt ctcgccataa acaaaagagt
    14641 tttgagcaaa tagtgccacc ttttttacga agggtgggca ctatttaact atttgttttt
    14701 cttttccttc ttccaacttg ctgcaagcca gtggggcgta gtatcaggtt tacagcctcg
    14761 gccatgttct gccccgctcc cctgcctttc aggtattcaa caacatcatt tcttgtaggg
    14821 gctgtttgtt gattatctgg atcatatgtt gcccaaagtt ctcttattgc gtcttctaca
    14881 tattcaagac cttcgctctt atgaccataa gtcagcaata ctgtttcttc agaattaatc
    14941 acgcgcgatt cagcatcaat tattactgat tggttagatg gcttaatctc cttatccgat
    15001 atagagcgag gaggtcttac ataatcgacc ctctcatttc ttacccattc aagtaaagaa
    15061 tgacgggtaa taattgtttc gtccatggat attttcttca aacgatctgg tatctctgta
    15121 cggggggtgc cggatgctct ttgtacttcc cacaaaggat aatattcaat ccacacagga
    15181 gttaatacac cacgacgaat tgcagtaacc attccattag ccaaccccca tgccattttc
    15241 caacgttcat gtttataatc tctggcatgt tcaagagtag ggtaatcata aggatcaatt
    15301 ccggccaaca ataaagcggc ttgggtgata gaaaactcct gtactgttcg ccagtgggat
    15361 aagtcaggaa tgttttccac aattggcata ttctccccct tcaagcggtc taatgttaag
    15421 gccgatccag ttcggtttgc atcagccttt ttagtcgctc aaagtctact actgtatact
    15481 caaccagtca tccacatttt ccaaacttgc catgtaccac attcccgccc tgttccagtg
    15541 agtccatata gtcggcgtac cactggagca tctcctgccg tccgtccagg tattgcgcgt
    15601 gattgtatgt gccacggatc gagtttttat cgacgtgcgc cagctgcgtt tctatccacg
    15661 cggagttata accctgctca tgcaaaatcg tactcatggt atgccggaac ccatgaccag
    15721 taacgcgccc tgcatagcca atacgacgaa taagcacgtt catagccatt tcactcatcg
    15781 gcttactgtg ctgtattcga ccagggaaaa taaacggata ctgccccgtc atgtgtttta
    15841 actgttccag aatagcgatc gcctgttctg aaagtggcac gcaatgagga cggcgcattt
    15901 tcattcgttc tttcggcact tcccacagac gattatcaaa atcaatctcc tgccactctg
    15961 cctgccgtaa ttcacctggg cggagtcctg taagaatcaa caatcgcata cccagcttga
    16021 cgatctggct tccggtgtac gtattaaaaa cgcggaaaaa ctccggtagc tcatcaactt
    16081 tcagatacgc gtaatgctct ttcttgtgtg gtgcaaatgc gcttgccaga tcaggagcgg
    16141 ggttatattc agcgcgtccg gtaattattg cgtaccggaa cacttcgcca catcgctgtc
    16201 gaaccttacg caatttttca gttgcgccac ggtcattaag acgagagaga accgcgagca
    16261 attccatcgg ttttatttct gctatcggtt tgctgccgat atacgggaat acgtccttct
    16321 caaacgtatc aatcatctct gcggcatatg attgtgacca tctgtccagg cgtttttcat
    16381 accactcacg ggcgatcgcc tcaaaggtgt taccgcattt gttcgcctgt tctcgctttt
    16441 gggcctttct gacctcactc ggattaactc caccagcgat aagccttttg gcttcatccc
    16501 ttgccgtcct ggcatcagaa agggacacct caggataaac acccagcgac aacattttgg
    16561 gcttgcctgc aaacctgtat cgcaatcgcc agcccttcga accgttcggc tcaatgagta
    16621 acgaaagccc gttgccgtca ttgagtgtat aggctttctc ttttggcttc gcgcgacgga
    16681 tagcaaggtc tgaaagcaac atgtgtatag cccccaaaaa acgattttgt cctatacgca
    16741 ttactataca cttctgtgtg tagatttagg tagatgactg tagacggttg tagacgagga
    16801 aaatcgctgg aggccttgat attcggggga ttggtagact ttagtagacg ttggtagatg
    16861 tttgtttgga gcgggcgaag ggaatcgaac cctcgtatag agcttgggaa gctctcgttc
    16921 taccattgaa ctacgcccgc ttcgagatgc gtaaggcatt ataaacctta cgctctcctt
    16981 agcaagtgcc gcgctgctga ctgctgatta attcgccatc agcattttgg cttgctgccc
    17041 tgcggcggca agtaacgtag cggatcaatt gccgttgcac ggtaacgaat ctggaaatgc
    17101 aggcgaacag atgccgcatc cgtgctcccc atagtggcga ttttttgccc agccttcacg
    17161 ctttgcccat tatttactag catcgtgtca ttatgggcgt aagccgtaat gtaatcttca
    17221 ctgtgtttaa tcatgatgag attaccgtag ccacgcagct ggttgcccac atacaccacc
    17281 tttcctgcac ccgcggcgta aataggtgta ccccgtggag ctgagatatc aatcccttta
    17341 ttgccgccat ctgctgtcga atacggcatg ataactttcc ctgtcgttgg ccataaccaa
    17401 caacgttgcc ctactggcgg ccaggaagat ttcggtaccg ctgatgacgg tgtaaccgat
    17461 gcggttttgg tcgttgattt ggcggttgat ttacgtgtac tactgctact tttcgcccca
    17521 cccagtttta gtttctgacc aacttcaatg gtgtaagggg gggaaatgcc gttcagtcgc
    17581 gccagttctt ttacgctggt tcccgtggtg cgcgaaatac gatatagcgt atccccccgt
    17641 ttcacggtgt aaacggagcc ggaatacgtt cctgtatcgg atgatttgct acccgaacag
    17701 cccgccaaaa gcagtccaac cgataacaac atcacgatac ccagagattt tttattcagg
    17761 cgtcccgcac tcaaaatctt tcctcgctta aaaaacaaga cgccttatca tagcagccag
    17821 cctcttgata cccaatctct tgcttctgga aaaacgtggt aatagagaaa gaggttaatt
    17881 tcatttggca aagggaagta tcatcagcta aatattcgct gtagaggtat cgaacttcct
    17941 gactttttac cctatttccc atcctgatgc gtaataatcc caatttatca cattgatttt
    18001 atcgccactc atattgatac gtatcactgc ccctattcag agtcctgttt cgtatcatat
    18061 ctgtgttttt ttaacgtcat cacacttcct acctccccta acgcttatcg tcgtttctgg
    18121 cgtaaatctt gcctgcttag actaaatctt tgccataaga accaaatgtt caaatcgagg
    18181 ggatttcaat ggaagcgcgg gaagcaaccg ctacgggtga atcatgcatg cgcgtcgatg
    18241 ccattgctaa ggtcaccggg cgggcacgat atactgacga ttatgttatg gcgggcatgt
    18301 gttatgcgaa atatgtacgt agccctatcg cacatggtta tgccgtaagt attaatgatg
    18361 aacaagccag aagtttgccg ggcgtactgg cgatttttac ctgggaagat gtgcctgata
    18421 ttccatttgc tactgctggg catgcctgga cacttgacga aaacaagcgc gataccgccg
    18481 atcgcgcact gctaactcgc catgttcgtc atcatggtga cgccgttgcc atcgtcgtgg
    18541 cccgcgatga actcacggca gaaaaagcgg cgcaattggt cagcattgag tggcaagaat
    18601 tacccgttat caccacgcca gaagcggcgc tggcagaaga cgctgcacca atccataacg
    18661 gtggcaattt actgaaacaa agcacgatgt cgacgggtaa tgtccaacaa acaatcgatg
    18721 ccgccgacta ccaggtacag gggcactatc agacccccgt tattcaacat tgtcacatgg
    18781 aaagcgtaac atcgctggcg tggatggagg atgactcgcg aattaccatc gtttccagca
    18841 cccagatccc gcacattgtt cgccgcgtgg ttggtcaggc gctggatatt ccctggtcat
    18901 gcgtacgagt catcaaaccg tttgtcggtg gcggttttgg taataaacag gatgtactgg
    18961 aagagccaat ggcggcattc ctgaccagca agcttggcgg cattccggtg aaagtttccc
    19021 ttagccgtga agagtgtttc ctcgcaaccc gtacccgcca cgcttttacc attgacgggc
    19081 aaatgggcgt gaaccgcgac ggaacattga aaggttatag tctggatgtt ctgtctaaca
    19141 ccggcgctta tgcatctcac gggcactcca tcgcttctgc gggggggaat aaagtcgctt
    19201 acctttatcc tcgttgtgcc tacgcttaca gttcaaagac ctgctatacc aacctcccct
    19261 cggctggtgc gatgcgtggt tatggcgcgc cacaagtcgt atttgccgtt gagtctatgc
    19321 ttgatgacgc cgcgacagcg ttaggtattg atcctgttga aattcgttta cgcaacgccg
    19381 cccgcgaagg agatgctaat ccgctcacgg gcaaacgtat ttacagcgca gggttgccgg
    19441 agtgtcttga aaaaggccgg aaaatctttg aatgggaaaa acgccgtgca gaatgccaga
    19501 accagcaagg caatttgcgc cgcggcgttg gcgtcgcctg ttttagctac acctctaaca
    19561 cctggcctgt cggcgtagaa atagcaggcg cgcgccttct gatgaatcag gatggaacca
    19621 tcaacgtgca aagcggcgcg acggaaatcg gtcagggtgc cgacaccgtc ttctcgcaaa
    19681 tggtggcaga aaccgtgggg gttccggtca gcgacgttcg cgttatttca actcaagata
    19741 ccgacgttac gccgttcgat cccggcgcat ttgcctcacg ccagagctat gttgccgcgc
    19801 ctgcgctgcg cagtgcggca ctattattaa aagagaaaat catcgctcac gccgcagtca
    19861 tgctacatca gtcagcgatg aatctgaccc tgataaaagg ccatatcgtg ctggttgaac
    19921 gaccggaaga gccgttaatg tcgttaaaag atttggcgat ggacgctttc taccaccctg
    19981 aacgcggcgg gcagctctct gctgaaagct ccatcaaaac caccactaac ccaccggcgt
    20041 ttggctgtac ctttgttgat ctgacggtcg atattgcgct gtgcaaagtc accatcaacc
    20101 gcatcctcaa cgttcatgat tcagggcata ttcttaatcc actgctggca gaaggtcagg
    20161 tacacggcgg aatgggaatg ggcattggct gggcgctatt tgaagagatg atcatcgatg
    20221 ctaaaagcgg cgtggtccgt aaccccaatc tgctggatta caaaatgccg accatgccgg
    20281 atctgccaca actggaaagc gcgttcgtcg aaatcaatga gccgcaatcc gcatacggac
    20341 ataagtcact gggtgagcca ccaataattc ctgttgccgc tgctattcgt aacgcggtga
    20401 agatggctac cggtgttgca atcaatacac tgccgctgac gccaaaacgg ttatatgaag
    20461 agttccatct ggcaggattg atttgaggat aacatcatgt ttgattttgc ttcttaccat
    20521 cgcgcagcaa cccttgccga tgccatcaac ctgctggctg acaacccgca ggccaaactg
    20581 ctcgccggtg gcactgacgt actgattcag ctccaccatc acaatgaccg ttatcgccat
    20641 attgttgata ttcataatct ggcggagctg cggggaatta cgctggcgga agatggctcg
    20701 ctacgtatcg gctctgcaac gacatttacc cagctaatag aagatcctat aactcaacgt
    20761 catctcccgg cgttatgtgc tgcggccacg tccattgctg gaccgcagat ccgtaacgtc
    20821 gctacctacg gtggaaatat ttgcaacggt gccaccagcg cagattctgc cacgccaacg
    20881 ctaatttatg acgcgaaact ggagatccac tccccgcgcg gtgttcgttt cgtcccgatt
    20941 aatggctttc acaccgggcc gggcaaagtg tctcttgagc atgacgaaat cctcgtcgcc
    21001 tttcattttc cgccacagcc gaaagaacac gcgggcagcg cgcattttaa atatgccatg
    21061 cgcgacgcaa tggatatttc aacgattggc tgcgccgcac attgccgact ggataacggc
    21121 aatttcagcg aattacgcct ggcatttggt gttgccgcgc caacgccgat tcgctgccaa
    21181 catgccgaac agactgcaca aaatgcgcca ttaaacctgc aaacgctgga agctatcagc
    21241 gaatctgtcc tgcaagatgt cgccccgcgt tcttcatggc gggccagtaa agagtttcgt
    21301 ctgcatctca tccagacgat gaccaaaaaa gtgattagcg aagccgtcgc cgcggcgggg
    21361 ggaaaattgc aatgaatcac agcgaaacaa ttaccatcga atgcaccatt aacgggatgc
    21421 cttttcagct tcacgccgca ccaggcacgc cgctctcgga attactccgc gaacaaggac
    21481 tgctaagtgt caaacaaggg tgctgcgtgg gtgaatgtgg tgcctgtacg gtgctggtcg
    21541 acggcacagc aatagacagt tgcttatacc ttgccgcctg ggctgaagga aaagagatcc
    21601 gcacgctgga aggtgaagcg aaaggcggaa aactttctca tgttcagcag gcttatgcga
    21661 aatccggcgc agtgcagtgc gggttttgta cgcctggcct gattatggct accacggcaa
    21721 tgctggcgaa accacgcgag aagccattaa ccattacgga aattcgtcgc ggactggcgg
    21781 gaaatctttg tcgctgcacg gggtatcaga tgattgtaaa tacagttctg gattgcgaga
    21841 aaacgaagta aaaggatatc cggcctgaat tcaggccgga ttcactgagg ttatgtgttt
    21901 aacaactcat atttcttaat cttgcgatag agcgtagcaa tgccgatgcc cagttcatca
    21961 gcaacttgct tcttgctgtt atgacgtgaa agcgcctcgc ggatcatttg cttttccatc
    22021 tcctccagcg ccgtgccgcc cgcatcatcg agtgacaggt gcgcctcact gacctctgtt
    22081 acatcacttt gctccgttgt gccattattc agcagatttg gcggcaatag cgtgctgtcg
    22141 ataacttcac ctgaaggaac tacgttaacc agatattcca tcaaattgct taactcgcgc
    22201 aggtttccgg gccaacgatg cttacgcaat atttcgacga catcgggagc aatgccagga
    22261 taaaccgatc ccagacgacg ggtatgcaga tgtaaaaagt aatgcaccaa tagttcaata
    22321 tcttcctgac gttcacgcag cggtggcaga gttatcggga taacattaag tcggtagaag
    22381 agatcttcgc ggaatttacc ttcggcaatg aactgggcca aattctgatt agttgcagaa
    22441 atgatgcgaa tgtcgacttg tattgggcta ctggcaccaa tcggcagaat ttcacgcgcc
    22501 tcaatagcgc gcagtaattt agcctgcaac attaatggca tatcacctat ttcatcgaga
    22561 aacagcgtgc ccgtattcgc cgcctgaatc aaccctgttt taccgttggc agaagcgcca
    22621 gtaaatgcgc ctttaacata accgaacagt tcgctttcca gaagctgctc cggaatcgcg
    22681 gcacagttga tagcaataaa gggtttattc cgtcttccgc tcaacttatg gattgctcgg
    22741 gcgacgactt ctttacccgt gccgctttca ccaaccacca taacgctgga tgggctgggt
    22801 gcaatacggc taatgagtcg ttttaattgc cgcataacac ggcactcgcc aaccaattgt
    22861 tcaatatgtg gttcatcagg tgcatttgct acagaaaaac tggtatgcga ttggtgaaat
    22921 gccattaaaa ataattgtcg gccctgaatg ttatgcaatt gaccaatgat taattcactt
    22981 ttatcgtccc acgaaacaat atgctgcata tgtccatgag taaaattact ctcaaatgtt
    23041 aatggtctga aacggatagg tttcccaata atattatttt gtacaacacc aagtgttttt
    23101 aaggcagtct gattaacaaa ctgaacccga ctttcatcat caacaactaa tacgccctga
    23161 tccatattat cgatcatggt cgcgaatatt ttactgatgt tatctcctag cccctgatcc
    23221 tccagaagtt tcgaaacaaa aatggtggat atatggcgaa cataatcaga aaattcgcgt
    23281 aaattatcac tgatatgctc ttgttgctcg tgggtaacgg caatcaaact tatcacccca
    23341 acacaacgat cctgtaaaat gacaggcgta cccagaaatg ctttttcgcg gcaattttct
    23401 ttactatcgc aaccttcgca aaggggatcg aagcgagact gtgtcacaac tttttcagtt
    23461 ttcgtttcca ggacgtggcg gagcaggcgt gagttgccgc tcaactggcg gccaagaaac
    23521 ttcccatacg cgccagttcc ggcgacgcga cacaagtttt catcaacgat ctcaacctca
    23581 agctgcaaaa cgctggcaag cattctggca aaacgctgaa ttgtcggttg aatttgcatc
    23641 aatactgact gcgtagtagc aagctccata gctttacctt ccagacttac ttaaaagtcg
    23701 atcattgaag acgttgatgg ttcacagatc atgatgatat taactcaggc gaaattggct
    23761 ttgataaaaa cataagattt ttatcacttt ctaatgaaat tatggaagag atatcacatt
    23821 tctatatcaa tatgagaatt acggcggtga gtttatcaaa ctgaagagag atagcctgtc
    23881 cctttatctt atttctgata cttagcagta aataaataac gcgataagaa aagccaatcg
    23941 ttttcgtatt ttacaaacaa cccgaagctg gcatcaattt gtgatcaacc ccacacatta
    24001 tccgtcaaat tagtctttta cagccccgtg gataattctg gcacacttat tgttagtccc
    24061 aggtatagct gtgaaaacac caatcacttt ggcaagtcac agtgaaataa accactttgc
    24121 ctgtcattcc actaccggga ctttatgatg aaaactgtta atgagctgat taaggatatc
    24181 aattcgctga cctctcacct tcacgagaaa gattttttgt taacgtggga acagacgcca
    24241 gatgaactga aacaagtact ggacgttgcc gcagcattaa aagcactgcg tgctgaaaac
    24301 atctcaacca aagtctttaa tagtggatta ggtatttccg tattccgcga caactccact
    24361 cgtacccgct tctcttatgc ttccgcgctt aacctgctcg gccttgcaca acaagatctc
    24421 gacgaaggca aatcacaaat cgctcacggc gaaaccgtgc gtgaaaccgc caatatgatc
    24481 tccttctgcg ccgacgctat tggtattcgc gacgatatgt atctgggcgc aggcaacgcc
    24541 tatatgcgtg aagttggcgc tgcacttgat gacggttaca agcagggtgt actgccacag
    24601 cgtccggctt tagtgaacct gcaatgcgat attgaccacc cgactcagtc aatggctgac
    24661 ctcgcgtggt tacgtgaaca ctttggttca ctggaaaacc tgaaaggtaa aaaaatcgcc
    24721 atgacctggg cctactctcc aagctatggc aaaccgctct ctgtaccaca aggcatcatc
    24781 ggtctgatga ctcgcttcgg tatggatgtc accctggccc atccggaagg ctacgacctg
    24841 atcccggatg tagtagaagt cgcgaaaaac aatgctaaag catctggcgg tagcttccgt
    24901 caggtcacca gcatggaaga agcattcaaa gatgcagaca tcgtttatcc gaagtcatgg
    24961 gcaccttaca aagtgatgga acagcgtacc gaattgctgc gcgctaacga tcacgaaggc
    25021 ttaaaagcac tggaaaaaca gtgtctggca cagaacgcgc aacacaaaga ctggcattgt
    25081 actgaagaga tgatggaact gacccgtgat ggcgaagccc tgtacatgca ctgcctgcca
    25141 gctgatatca gcggcgtatc ctgcaaagaa ggggaagtga ctgaaggcgt attcgaaaaa
    25201 taccgtatcg ctacctacaa agaagccagc tggaagcctt atatcatcgc cgcgatgatc
    25261 ctgtcccgta aatacgccaa accaggtgca ctgctcgagc aactgctgaa agaagcgcaa
    25321 gaacgcgtga aataacccct cgggccgatc agctggtcgg ccctctattt ccagaggcca
    25381 aaaggatagg atatgtccgt tttctcattg aagattgata tcgccgataa caaatttttc
    25441 aacggcgaaa catcaccgct cttttcgcaa agccaggcca aactggcgcg ccagttccac
    25501 cagaaaatag ctggttatcg cccaacaccg ctttgtgcgc tggacgatct cgcaaacctt
    25561 tttggtgtga agaaaattct cgtcaaagac gaatcaaaac gattcggtct gaacgccttc
    25621 aaaatgcttg gcggtgcgta cgccatcgct caattattgt gtgaaaaata tcatcttgat
    25681 atcgaaacgc tgtcatttga gcacctgaaa aatgccatcg gcgaaaaaat gactttcgcg
    25741 accaccaccg acggcaacca cgggcgcggt gtggcgtggg cagcacagca actcggacag
    25801 aatgcggtga tttacatgcc gaaaggttct gctcaggaac gcgttgacgc cattctgaac
    25861 ctcggtgccg agtgcatcgt cacggatatg aactatgacg ataccgttcg cctgaccatg
    25921 caacacgcgc agcagcacgg ctgggaagtg gtacaggaca cggcatggga aggttacacc
    25981 aaaatcccaa cctggatcat gcaaggctac gcaaccctgg cagatgaagc cgtcgagcaa
    26041 atgcgtgaaa tgggcgtaac cccgacgcac gttctgctgc aagccggtgt cggagcaatg
    26101 gccggtggtg tgctgggtta tctggtcgac gtctacagcc cgcaaaatct gcacagcatt
    26161 attgttgaac ctgacaaagc tgactgtatt tatcgctccg gcgtcaaagg cgacatcgtc
    26221 aacgttggcg gtgatatggc caccatcatg gcgggcctgg cctgtggcga acctaacccg
    26281 ctgggctggg aaatcctacg taactgcgcc acccaattca tctcctgcca ggacagcgtt
    26341 gccgcattag gtatgcgcgt gctgggtaat ccgtacggca acgacccgcg catcatctcc
    26401 ggtgaatccg gcgctgtcgg tttgggcgtt ctcgcagcgg ttcattatca cccgcaacgt
    26461 caaagcctga tggaaaaact ggcgctgaac aaagatgccg tagtgctggt tatcagcact
    26521 gaaggcgaca ccgacgtgaa gcactaccgc gaagttgtct gggaaggcaa acacgctgta
    26581 gcaccttaat caccctattg gaactactcc ctgcaaaacg gggagtaaaa aatctggaga
    26641 aaaataatgg ctaagaatat tccattcaaa ctgattcttg aaaaagcaaa agattaccag
    26701 gcggacatga ctcgcttcct gcgcgacatg gttgctattc ccagtgaaag ctgcgacgaa
    26761 aaacgcgtag tacatcgtat taaagaagag atggaaaaag tcggcttcga taaagttgaa
    26821 atcgacccga tgggcaacgt tctcggttat atcggccacg gcccgcgtct ggtggcaatg
    26881 gacgctcata tcgataccgt cggcattggc aacatcaaaa actgggactt cgatccgtac
    26941 gaaggcatgg aaactgatga gctgatcggt ggtcgcggta cttccgacca ggaaggcggc
    27001 atggcatcta tggtttatgc cggtaaaatc attaaagacc tcggtctgga agatgaatat
    27061 accctgctgg ttaccggtac tgtgcaggaa gaagactgcg acggtctgtg ctggcagtac
    27121 attattgaac aatccggcat tcgcccggaa tttgtggtca gtaccgaacc aaccgactgc
    27181 caggtatacc gtggtcagcg cggtcgtatg gaaattcgta ttgatgttca gggtgttagc
    27241 tgccacggtt ctgcgccaga acgcggtgac aacgccattt tcaaaatggg tccgattctt
    27301 ggcgaattac aagaactctc ccaacgtctg ggttatgacg aattcctcgg caaaggcacc
    27361 ctcaccgttt ctgaaatctt cttcacatcc ccaagccgtt gcgctgtagc agatagctgc
    27421 gccgtctcta ttgaccgccg tctgacctgg ggcgaaacct gggaaggcgc gctggacgaa
    27481 atccgcgccc tgcctgcagt acagaaagct aacgcggttg tttctatgta caactacgac
    27541 cgtccgtcct ggactggcct ggtttaccca accgaatgct acttcccgac ctggaaagtg
    27601 gaagaagatc acttcaccgt taaagcactg gtgaatgcct acgaagggct gtttggcaaa
    27661 gcgccggttg ttgataagtg gaccttctca actaacggcg tatctatcat gggccgtcac
    27721 ggcattccgg tgatcggctt tggcccgggt aaagaacctg aagcgcatgc acctaacgaa
    27781 aaaacctgga aatctcacct ggtgacctgt gccgcgatgt acgctgcaat cccgttaagc
    27841 tggctggcaa cagagtaatt actcttcact tattcccctc cggttcgccg gagggttttt
    27901 ggagtttgct atgcgcgtat tgatcaaaaa cggcactgtc gttaacgcag atggacaagc
    27961 caaacaggat ttgctgattg aaagcgggat tgttcgccag ttgggcaaca atatttcgcc
    28021 gcagctcccg tatgaagaaa ttgatgccac tggctgttac gttttccctg gcggcgtgga
    28081 tgtccatacg catttcaata ttgatgtcgg catcgcgcgc agttgtgatg atttttttac
    28141 cggtacccgc gcagctgcgt gtggcggtac aacaaccatt attgaccata tgggatttgg
    28201 cccaaacggc tgtcggttac gccatcaact ggaggtttat cgtggttatg ccgcccataa
    28261 agcggtcatc gattacagct ttcacggtgt gatccagcac attaatcacg caatcctcga
    28321 cgaaatcccg atgatggtcg aggaaggact gagcagtttt aaactctatt taacctatca
    28381 atacaaactc aacgatgacg aggttttgca ggcattacgc cgtctgcatg aatccggcgc
    28441 gctgaccacc gtgcacccgg aaaatgatgc ggctatcgcc agcaagcggg cggaatttat
    28501 cgccgcaggg ttaaccgcgc cgcgctatca tgccttgagt cgccctctgg aatgcgaagc
    28561 ggaagccatc gcccgcatga ttaacctggc acaaattgcc ggtaacgccc cgctctatat
    28621 cgtgcacctg tctaacggct taggtctgga ttatctgcgt cttgcccgtg cgaatcacca
    28681 gccagtctgg gttgaaacct gcccacaata tctcctgttg gacgaacgca gttacgatac
    28741 agaagacggc atgaagttca ttcttagccc accgctgcgt aacgtacgcg agcaggacaa
    28801 actgtggtgt ggcatcagcg atggtgcgat tgacgtggtg gcgaccgatc actgcacctt
    28861 ctcgatggct caacgcctgc aaatttctaa aggcgatttc agtcgctgcc caaatggctt
    28921 acccggtgtg gaaaaccgca tgcagttact gttttccagt ggcgtgatga cgggacgtat
    28981 aacaccggaa cgctttgttg aattaaccag cgcaatgccc gccaggctgt ttggcctgtg
    29041 gccgcaaaaa ggattattag cgcccggttc cgatggcgac gtggtgatta tcgacccacg
    29101 tcagagccaa caaattcagc atcgccatct ccacgacaac gccgactact cgccatggga
    29161 gggttttacc tgtcagggcg cgattgtcag aaccttatcc cgtggtgaaa cgattttctg
    29221 tgacggcacc tttacaggca aagccgggcg aggtcgtttc ctgcgacgca aaccgtttgt
    29281 ccctcccgtg ctctaaccac tgtcagggat ctatacacaa tcaagaatga tgagtgagga
    29341 aaaatgagta agaaaattgt tctcgccctg ggcgggaatg cgctgggcga tgacctggcc
    29401 ggacaaatga aagcggtaaa aattacttct caggcaattg ttgatttaat tgcccaagga
    29461 catgaagtta tcgtcactca tggtaacggg ccgcaggtgg gcatgattaa ccaggccttt
    29521 gaagctgctg caaaaactga agcgcactcg ccaatgctgc cgatgtctgt ctgcgttgcc
    29581 ttaagccagg gttatattgg ttacgatcta caaaacgcct taagagaaga actgctttct
    29641 cggggcatta ataaaccggt agcaacgctg gttactcagg tcgaagtcga cgctaacgat
    29701 ccggcattcc tcaacccgac caagccgatc ggttcgttct ttaccgagca agaagcagag
    29761 caactgacaa aacagggtta cactctgaaa gaagatgctg gtcgcggcta tcgccgtgtt
    29821 gttgcctcgc caaaaccggt tgatattatt gaaaaagaaa cggttaaagc tctggtagat
    29881 gcaggccagg tggtgatcac cgttggcggt ggtggtattc ctgttattcg tgaaggtaac
    29941 catctgcgtg gtgccagcgc ggttatcgat aaagactggg ccagcgcccg tttagcagaa
    30001 atgattgatg ccgatatgct gatcattctg actgcggtag aaaaagtggc cattaacttc
    30061 ggtaaagaga atgaacagtg gctcgatcgc ctgtcattaa gtgatgctga acgctttatc
    30121 gaagaaggtc attttgcgaa aggttctatg ctgcctaaag tggaagctgc cgcctcgttt
    30181 gctcgctctc gcgcaggtcg tgaagcactg attaccgtat tgagcaaagc gaaagaaggg
    30241 attgaaggca aaaccggaac ggtgatttgt cagtaatttt aaggccgggt gggtaatacc
    30301 atccggccat tttcatttaa gccacttcca gcacttcttt tgtcgctttc acgccctgat
    30361 gcatcaacat cattaacgcc tcaagtacgc cgccgccaat cgcccgggct ttatcagaaa
    30421 cgctggagaa atcagcggtt tcaccacgag gatcgatatc accaatttta aaaccaccaa
    30481 ccactgccaa gccgtcgttc aacaagccac gcaccatccc cgtcaacggt gctttaattt
    30541 catgctcacc aatccaggca atcacatcgc cctctttcac cagatcgcct aatttcacgt
    30601 tggatcgcat aatgcctgca gcaggagcac ggatcacccg tcgggtggta tgccccataa
    30661 tattgccagg aacaccggta ttctcctgcg cacaaccaga gtaaatcacc tgaccgagcc
    30721 agtgcccgcg atttgtttca attaccgcat gacaatcctt ccctgcagta aagcccggcc
    30781 caagcgcgat tgttactggt gccatatctg cccgcgttcc caaattctgt ttcgccagaa
    30841 tagcgtccac cacgcaaagc ggtttcagtt catcaagcaa tgaacaggcg ggatctacca
    30901 tcacagggat gaatccgcgt tcggtaagtt tcatcgcttc cgcagagctg gttgccaggc
    30961 gagcagtgac gccttcgacc gtcatttcgc catcgaacac ggcctgggca aacgccaccg
    31021 tacaacgaat cactgtcggt ttttccactt ccagcatgat cactttaaaa cccgcatgat
    31081 acagacgtag cgccacacca ctggcgatat ctcccgcacc gcgaatcacc accagctggt
    31141 gtccggaagg atgagaaggt ttcatcatca gcccacccgg cgcgttattt ttcacctgta
    31201 atatttctgc caacacgctg atggcgatct cctgcggcgt ttccgcacct atgttgtaac
    31261 caacgggcgc atgtaaacgg gcaatatgtt cttcagccac gcctttctca cgcaattgac
    31321 gcaggaaaag ctgaaccttg cggcgacttg ccagcaaccc caaccatgca atgggctgtt
    31381 caatgagttt gtcgagggct tcacgatcct gattattcgt ggcaatcagg acaaaattat
    31441 cagggcgaat atccagtgct tccactgctg caccaaacga ctcagcatgg agaagcatgg
    31501 ttgatggagg gaatagttca ggattgaggc tttcgcgata aatatcggca acggcaatat
    31561 caaatcctaa tagcgccgca ctctgggcta tcgcccggtt gacatgcccc gcgccaatca
    31621 acaccagacg tggacgcata ccatggacgc tgataaatac tgacattgca cctccacaat
    31681 ctgacccgac agcatccgca ccgttacgag ccatacgtcc atggaataat cgcggcttac
    31741 gttcctgcaa tgcctgaagt gactcttcaa tcaccttccg ctcaaccatt ccgccaccaa
    31801 ttgtaccgac gatagaacca tcggcgcgca ctaacatttg tgcagaatgg cggggagtcg
    31861 agcctcggct atcaacaatt tgcgccatcg caaacggaca attttgctct tcgagttttg
    31921 cagcctctgt gaaaatattc ataccaacct caatggatat cctttcagta acccggaata
    31981 cccgggccgc taattcacaa atctgcgcgc gattgcagga tgttcttgta tatcgcccag
    32041 ccagattgcc tctacgtcgt gttgttgcag cggttgaagg agctcgcttt gtgcaatcgc
    32101 attctcacat tgagaaaaac ggttaatgaa ccagactcgc cgacaacctt gcggtacgtt
    32161 tttaaacgcc ccctgagggt ggcgaaccag cgcaacgaga tcgctcagtt gcaaggttgc
    32221 atcaggtgtt aacccggtaa tatcagcaaa ctgcgaccag cgatggacat tttctgtgct
    32281 cactttcgcg cccaaagtat gtcctcccat cacagcaatc acgcagcaac tgcttttggg
    32341 tatgcaaggt tcgtgctcat caggcgcttt taacggcatt ccacgcgagc catctgcttc
    32401 aatgagaatt acgtcacact ctggtcgttg caccagtgca tcaatcgctt ctggcgtaaa
    32461 tccctgcact tttccctggt tcgctttcca gctgtgaaaa caaaatgaaa tgggggatgt
    32521 aagagacgca tgaggaagca tggcgggatc acgacagaaa accacgggcc agtgagacgt
    32581 tggcataaac atatgtgtcg tcgtcgtgat taacacacgc ctgccgctag cctggagcag
    32641 ttctgccagc caaaaaagca agctggtttt ccccccagca ccaacaacag aaatcacagt
    32701 gggacgtttc tgcgcaccta aatcaatgac taatgccgat gggtcaacta tacttttcac
    32761 gagtctttat gacctctctg ggataaatta tccccaactt aatccatcag gaagtaacgc
    32821 aattatcagg cgttattagc ccctataaat aatggaacca ctatgtcagc catcgactgt
    32881 ataattaccg ctgccggatt atcatcaaga atggggcaat ggaaaatgat gttaccctgg
    32941 caacagggga caattcttga tacaagtatc aaaaatgcgt tgcagttttg tagccgaatt
    33001 attttagtca ccggctatcg tggtaatgaa ctgcacgaac gttatgcgaa ccagagcaat
    33061 atcactatta tccacaaccc agattatgcg cagggtttac taacatcagt aaaggccgca
    33121 gtacccgcag tgcaaacaga acattgtttt ctcacccacg gtgacatgcc aaccctcacc
    33181 atcgatattt ttagaaaaat ctggtcgtta cgaaatgatg gcgcaatact gccactccat
    33241 aatggcatcc ccggccatcc gattttagtg tcaaaaccat gcttgatgca ggcaatccaa
    33301 cgacccaatg tcaccaatat gcgtcaggca ctccttatgg gagctcatta ttccgtcgag
    33361 atagaaaatg cagaaataat tttagatatt gatactccgg acgactttat tacagcgcaa
    33421 aaaaggtata ctaaaattta gaaaatgtag ttaaacgatt gcgttcaaat atttaatctc
    33481 tccagcactc tttatcataa tgataaggaa tcactgattt tgagaaaaga gaaaaagtga
    33541 taaatgggct gacaataata attttcagga gtgattttaa ttctcatttg gcacctgtga
    33601 gttcattcac aaaaagcctt taagtttcga gccgcctcac atttttttat atttcccgcc
    33661 aaacctggca agagtggtgc gattgttgct ctatccccct aaaccaccgg atttctcaac
    33721 accggtcact caatgatatc tgtataagct aaggagaggg ttatggggga tattatgcgt
    33781 cccattccgt ttgaggaact tttgacgcgc atatttgatg aataccaaca acaacgctca
    33841 atctttggta ttcccgagca acagttttac tcacctgtaa aaggtaaaac tgttagcgtc
    33901 ttcggtgaaa cctgtgccac tcccgtcggc cctgccgctg gcccgcacac gcagctcgcg
    33961 caaaatattg tcacttcctg gctgactggc ggacgcttca tcgaactaaa aaccgtccaa
    34021 attcttgacc gcctggagct ggaaaagccc tgtatcgatg ccgaagacga gtgctttaac
    34081 accgaatggt ctaccgaatt taccctgctt aaagcctggg atgaatacct caaagcctgg
    34141 tttgccctgc accttctcga agcgatgttc cagccttctg attccggtaa atcgttcatc
    34201 tttaatatga gcgtcggtta caacctcgaa ggtattaagc aaccgccaat gcaacagttc
    34261 atcgacaata tgatggacgc atctgaccat ccgaaattcg cgcaatatcg cgatacgctg
    34321 aataaattac tccaggatga cgcattttta gcccgccacg gattgcagga aaaacgcgaa
    34381 agcttgcaag ccttacccgc tcgcatcccc accagtatgg tgcagggcgt caccctctcc
    34441 accatgcacg gctgtcctcc gcatgaaatc gaagccattt gccgctacat gctggaagaa
    34501 aaagggctca acacctttgt gaaacttaac ccgaccttac tgggatacgc gcgtgttcgt
    34561 gagatcctcg atgtctgcgg tttcggttac ataggcttaa aagaagagtc atttgatcac
    34621 gacctcaagc tgacgcaagc actggaaatg ctggaacgcc tgatggcact ggcaaaagaa
    34681 aaatcactcg gctttggcgt aaaactgact aacactctcg gcaccatcaa caataaaggc
    34741 gcactgcctg gtgaagagat gtatatgtca ggccgtgcgc tgttcccgct ctccatcaat
    34801 gttgcagcag ttctctctcg cgcctttgac ggcaaactgc ccatttctta ttccggtggt
    34861 gccagtcagc tgactatccg cgatattttt gatacaggta ttcgccctat tactatggca
    34921 accgacctgc tgaaacctgg cggctatttg cgcttaagtg cctgcatgcg cgagctggaa
    34981 ggctccgacg cctggggact tgaccatgtt gacgtcgaac gactgaacag actggcagca
    35041 gatgcgttaa ccatggaata cacccagaaa cactggaagc cagaagagcg tattgaagtg
    35101 gcagaagacc tgccgctgac cgattgctac gttgccccct gtgttactgc ctgcgctatc
    35161 aagcaagata ttccggaata catccgtctg cttggcgaac accgctatgc cgacgcgctg
    35221 gaactcatct accaacgcaa cgctctgccc gccattaccg gtcatatttg cgatcaccag
    35281 tgccaataca actgtacccg cctggattac gacagtgcgc tgaatatccg cgaactgaaa
    35341 aaagtcgcgc tggaaaaagg ttgggatgaa tataagcaac gctggcacaa accagccggt
    35401 tctggttcac gccatccggt tgccgtgatt ggtgcaggtc cggcgggtct ggcagcaggt
    35461 tacttccttg ccagagcggg ccatccggtt acgctgtttg aacgcgaagc caatgcgggc
    35521 ggcgtggtga aaaatatcat tcctcagttc cgaattccgg ctgagttaat tcagcacgat
    35581 atcgattttg ttgccgatca cggcgtgaaa tttgagtatg gctgctcacc cgatttgacc
    35641 gttgaacagt taaaaaatca gggcttccac tatgttctga ttgccaccgg cactgataaa
    35701 aatagcggtg tgaaactggc gggtgacaac caaaatgttt ggaaatcact ccccttcctg
    35761 cgtgaataca acaagggtac agcgctcaag ctgggcaaac atgtggtcgt tgtcggggcg
    35821 ggtaacaccg caatggactg cgctcgtgcg gcgttacgcg ttccaggcgt agaaaaagca
    35881 acgatcgttt accgtcgttc actgcaagag atgcccgcat ggcgcgaaga gtatgaagaa
    35941 gcgttgcacg acggcgtgga gttccgtttc ctgaataatc cggaacgttt cgatgctgat
    36001 ggcaccttaa ccttgcgcgt tatgtcgctt ggcgaacccg atgagaaagg ccgtcgccgt
    36061 ccggttgaaa ccaacgaaac agtaacactg catgtagaca gcctgatcac cgccattggt
    36121 gaacagcagg atactgaagc cctgaatgcg atgggcgtgc cgctggacaa aaacggctgg
    36181 ccagacgtcg accacaatgg cgaaactcgt ctgactgacg tctttatgat cggcgacgta
    36241 cagcgcggac catcctccat tgtcgctgct gtcggaaccg cgcgtcgggc gaccgatgcc
    36301 atccttagtc gggaaaatat ccgttcccac cagaacgata aatactggaa caacgtcaat
    36361 ccggcggaaa tctatcaacg taaaggcgat atctctatca ctctggtgaa cagtgacgat
    36421 cgtgacgcgt ttgtcgccca ggaagccgct cgctgcctcg aatgtaacta cgtttgcagc
    36481 aagtgtgtgg atgtctgccc gaaccgcgcc aacgtctcca ttgcggtccc aggcttccag
    36541 aatagattcc agacgctgca cctcgacgct tactgtaacg aatgcggcaa ctgcgctcag
    36601 ttctgtccgt ggaacggtaa accatacaaa gacaaaatca ccgttttcag cctggcgcaa
    36661 gactttgata acagcagcaa cccaggcttc cttgtggaag attgccgggt acgagtacga
    36721 ctgaataacc aaagctgggt gttaaacatc gacagcgaag gtcagttcaa caacgtacca
    36781 ccggagctga acgatatgtg ccgcatcatc agccatgtcc accagcatca tcattatctg
    36841 ctgggccgcg tggaggtgta atcatgttga ttctgaagaa tgtcaccgca gtgcagttac
    36901 acccggcgaa agtgcaggaa ggcgttgata tcgctatcga aaatgatgtg attgtcgcta
    36961 tcggcgatgc cctgacgcaa cgctaccccg atgccagcta caaagagatg catggtcgga
    37021 ttgtgatgcc gggaattgtc tgctcgcaca accattttta ctcggggctt tcccgcggaa
    37081 ttatggcaaa catcgccccc tgcccggatt tcatctcaac gctgaaaaat ctctggtggc
    37141 ggctcgatcg cgcccttgat gaagagtcgc tctattacag cgggctgatt tgttccctgg
    37201 aagcgattaa gagcggatgt acatcggtta tcgatcacca tgcctctccg gcgtatatcg
    37261 gcgggtcgct ctccacattg cgcgacgcat ttttaaaagt tggcctgcgc gcgatgacct
    37321 gttttgaaac tactgaccgt aacaacggca tcaaagagtt gcaggaaggt gtagaagaaa
    37381 acatccgctt cgcccgtcag attgatgagg cgaagaaagc agcaaccgag ccgtatctgg
    37441 tggaagcaca tatcggtgct cacgcgccgt ttaccgtgcc agatgccggt ctggagatgc
    37501 tacgtgaagc cgtgaaagcc acaggccgtg gtttgcatat tcacgctgcg gaagaccttt
    37561 acgacgtttc ctacagtcac cactggtacg gcaaagacct gctggcacga ctggcgcaat
    37621 tcgatctcat cgatagcaaa acgctggtcg ctcatgggct gtacttgtcg aaagatgaca
    37681 tcgccctact caatcagcgc gatgcgttcc tggtgcataa cgcccgttca aacatgaaca
    37741 accatgtcgg ctacaaccat caccttagcg acatccgcaa tctggcgttg ggaacggacg
    37801 gcattggttc ggacatgttt gaagagatga aatttgcctt ctttaaacat cgcgatgcgg
    37861 gtggtccgct gtggcctgac agttttgcca aagccctgac taacggtaac gaactgatga
    37921 gccgcaactt tggcgcgaaa tttggacttc tggaagccgg ttacaaagct gatttaacca
    37981 tttgcgatta caactcaccg acgccgctgc tggcagacaa tatcgccggg catatcgctt
    38041 tcggtatggg ctcaggcagc gttcacagcg tgatggtcaa tggtgtgatg gtctatgaag
    38101 accgtcagtt taacttcgat tgcgattcca tttatgcaca agccagaaaa gccgctgcca
    38161 gtatgtggcg tcggatggat gcgctggcat aaatgacaga tgccctcttc ccgcagggaa
    38221 gagggttaaa taaggaaaga agatgattga acaatttttc aggcccgact ctgtcgaaca
    38281 ggcgctggaa ctgaagcgcc gctaccagga tgaagccgtc tggttcgccg ggggcagcaa
    38341 actcaatgct acaccaaccc gtaccgataa aaagattgcc atttccttac aggatctgga
    38401 actggactgg gttgactggg ataacggtgc actgcggatt ggcgcaatgt ctcgcttgca
    38461 gccactgcgt gatgcgcgat ttattcctgc agcgctgcgt gaagccctcg gttttgttta
    38521 ctcacgccat gttcgtaatc agtcgaccat tggtggtgaa atcgccgccc gccaggaaga
    38581 gtcggtgctg cttcccgtcc tgttggcact ggatgctgaa ctggtttttg gcaacggcga
    38641 aacgctgtca atcgaggact acctggcctg cccatgcgat cgcctgctaa ccgaaattat
    38701 cattaaagat ccgtatcgca cctgtgcgac ccgcaaaatt agccgttctc aggcaggttt
    38761 aaccgtcgtg acggcagccg ttgcaatgac agaccacgac ggtatgcgaa ttgcgctgga
    38821 tggcgtcgcc agtaaagcac tgcgtctgca tgatgtcgaa aaacaaaatc tggaaggcaa
    38881 tgcacttgaa caggctgtcg ccaacgccat tttcccgcag gaagatttgc ggggcagcgt
    38941 ggcctataaa cgctatatca cgggagttct ggtagccgac ctgtatgccg actgccaaca
    39001 ggctggggag gaagccgtat gatcatccac tttactttaa atggcgcgcc tcaggagcta
    39061 accgttaatc caggcgaaaa cgtgcaaaag ctgttgttta acatgggaat gcactctgta
    39121 cgcaacagtg atgatggctt cgggtttgcc ggttctgacg caataatctt taacggtaat
    39181 atcgttaacg cgtccttgct tattgccgca cagttagaga aggcagatat tcgtaccgca
    39241 gaatctttgg gcaaatggaa cgagttaagt ctggttcaac aggcaatggt tgatgttggc
    39301 gtggtgcagt ctggttataa cgatccagct gcagctctga ttatcaccga tcttctcgat
    39361 cgcatcgccg cacctacccg cgaagagatc gacgacgcgc tttctggttt gttcagccgc
    39421 gatgcaggct ggcaacaata ctatcaggtc attgaactgg cggttgcacg taaaaataat
    39481 ccgcaggcca ccattgatat cgctccgact ttccgtgacg accttgaagt cattggcaag
    39541 cattatccta aaactgatgc cgcgaaaatg gtgcaggcga aaccctgcta tgttgaagac
    39601 cgcgtaacgg ctgacgcctg cgtcattaaa atgttacgta gcccacacgc tcacgcactg
    39661 attactcatc tggatgtcag caaagctgaa gccttaccgg gcgtcgttca cgttattact
    39721 cacctgaatt gcccggatat ctactatacc ccgggtggtc agagcgcacc ggaaccgtca
    39781 ccgcttgacc gccgtatgtt cggcaagaaa atgcgtcacg tcggcgatcg tgttgctgcg
    39841 gtcgtcgcag aaagtgaaga aattgcgctc gaagcattga agctcatcga cgttgaatat
    39901 gaagtgctta agccggtaat gtcgatcgac gaagcaatgg cggaagatgc gcctgtcgtg
    39961 cacgatgaac cggtggtgta tgttgctggt gcgccagata ctctggaaga cgataacagc
    40021 catgcagccc agcgcggcga gcatatgatc atcaacttcc cgatcggttc tcgccctcgc
    40081 aaaaatatcg ccgccagtat tcatggtcat attggcgata tggacaaagg ctttgccgat
    40141 gccgatgtga tcattgagcg aacctataac tcaacgcagg cacagcagtg cccgactgaa
    40201 acacatatct gctttacccg gatggacggc gatcgtctgg ttatccacgc ctccacccag
    40261 gtaccatggc acttacgccg ccaggtcgcg cgcctcgtgg gcatgaaaca gcataaagtt
    40321 catgtcatta aagagcgagt tggcggcggt tttggttcca aacaggacat cctgctggaa
    40381 gaagtgtgcg cctgggcaac ctgcgtgacc gggcgtccgg tactgttccg ctacacccgt
    40441 gaagaagagt ttattgctaa cacctctcgt cacgtcgcga aagtcaccgt caaactggga
    40501 gcgaaaaaag atggtcgcct gacggcagtg aagatggatt tccgcgccaa cactggccct
    40561 tacggcaacc actcactcac cgtaccgtgt aacggaccgg cgctgtcgct gccgttatat
    40621 ccgtgcgata acgtcgattt ccaggtcacc acctactaca gcaacatttg cccaaatggt
    40681 gcttatcagg gttatggcgc accgaaaggt aacttcgcta tcaccatggc attagcggaa
    40741 ctggctgaac agttacagat cgaccaactg gaaattatcg aacgtaaccg ggtacacgaa
    40801 gggcaagagc tgaaaattct cggtgcaatc ggtgaaggta aagcgccgac ctccgttcct
    40861 tccgccgcca gctgtgcact agaagagatc ctgcgtcagg gtcgcgagat gatccaatgg
    40921 tcgtcaccga aaccgcaaaa tggtgactgg cacatcggtc gcggcgtcgc cattatcatg
    40981 cagaaatcgg gtatcccgga tatcgatcag gctaactgca tgatcaaact ggaatcggac
    41041 ggcaccttta tcgttcattc tggcggtgcg gatattggta ctggtctgga tactgtagtg
    41101 acgaaactgg cagcagaagt gctgcactgc ccaccgcagg acgtgcatgt tatctccggt
    41161 gataccgatc atgcgttgtt tgataaaggc gcatatgcct cgtccggtac ttgcttctca
    41221 ggtaacgcgg cgcgtttggc agcggaaaat ctgcgggaga aaattctgtt ccacggcgcg
    41281 caaatgttgg gtgaggcagt ggcagatgtt caactggcaa cgccgggcgt cgtgcgcggc
    41341 aagaaaggcg aagtcagctt cggggatatt gctcataaag gcgaaaccgg caccggcttt
    41401 ggttcactgg tgggaactgg cagttatatc acgcctgatt tcgccttccc gtatggcgca
    41461 aacttcgctg aagttgccgt caacacgcgt acgggtgaaa tccgcctgga taaattctac
    41521 gccttgctgg actgcggtac accggtcaac ccggaattag cattgggaca aatctacggt
    41581 gctaccctgc gagctatcgg ccacagtatg agcgaagaga tcatttatga cgccgaaggt
    41641 cacccgttaa cgcgtgattt acgcagttac ggcgcaccga aaattggtga cattccgcgt
    41701 gatttccgcg ctgtgctggt gccgagcgac gataaagtcg gcccgttcgg ggcgaaatcg
    41761 atctcggaaa tcggtgtaaa tggcgcagct ccggcgattg ctaccgcaat tcacgatgca
    41821 tgcggcatct ggttacgcga atggcatttc acaccggaga aaatactcac tgcgctggaa
    41881 aaaatataaa taataaatgg ccgcttcggc ggcctgtttc tctgtttgga cgatgactaa
    41941 atagtgactg cgaaaatgag ataagccgtt ctcaaggcgt cctgcgcccc aaattcgtgg
    42001 gaatttattt tactaattca gatgatcaaa tttactttaa aaggagtgaa gggatgtctg
    42061 atataaacaa tgcaggttct gaccttatat ttgaactgga ggatcgccct ccctttcatc
    42121 aggctctcgt tggtgccatt acccatctgt tggcaatttt cgttccgatg gtaacccccg
    42181 cgttaatcgt gggggcggcc ttacagcttt ccgctgaaac aactgcctat cttgtttcta
    42241 tggcgatgat cgcctctggt attggtacct ggttacaagt aaaccgctac ggcatcgtcg
    42301 gttctggcct actctcaatt cagtcagtca atttttcatt tgttacggtc atgattgcgc
    42361 tgggcagcag catgaaaagc gacggttttc acgaagagtt aatcatgtcg tcgcttctcg
    42421 gcgtctcctt cgttggcgca tttctggttg tcggatcttc atttatcttg ccctatttac
    42481 gtcgggttat tacgcctacc gtcagcggta ttgtggtact gatgatcggc ttaagcctga
    42541 ttaaagtcgg cattatcgat tttggtggag gatttgcagc caaaagcagc ggtacattcg
    42601 gcaattacga acatctcggc gttggtttat tggttttaat tgtggtgatc ggctttaact
    42661 gctgtcgcag tccgttgcta cgcatgggag ggatcgccat tgggctatgt gtcggctata
    42721 tcgcatcgtt atgcctgggc atggtggatt tcagcagtat gcgcaatttg ccgttaatca
    42781 ccatcccgca tccgttcaaa tacggcttta gttttagctt ccatcagttc ctggtggttg
    42841 gcacgattta tctgcttagc gtgctggaag ctgtcggcga tatcaccgcc acggcaatgg
    42901 tttcccgccg acccattcag ggagaagagt atcagtcccg actgaaaggc ggcgtgctgg
    42961 cagacggtct ggtttctgtt atcgcctccg ctgtcgggtc attaccctta accacgtttg
    43021 cgcaaaataa tggggttatt cagatgactg gcgtcgcttc acgttatgtc gggcgaacca
    43081 tcgcggtaat gctggttatc ctcggtttgt ttccgatgat tggcggcttc ttcacgacca
    43141 ttccctcggc agttctggga ggcgcaatga cgttgatgtt ttccatgatt gccatcgctg
    43201 ggattcgcat aatcatcacc aacggtttaa agcgccgaga aacacttatt gtcgccactt
    43261 ctttaggttt aggacttggc gtctcctacg atcccgaaat ttttaaaata ttgccagcct
    43321 ctatttatgt actagttgaa aaccctattt gtgctggcgg gttaactgcg attttattaa
    43381 atattatcct ccctggtggc taccgacagg aaaacgttct gcctggtatt acctcagcgg
    43441 aagagatgga ttaacagtaa aggagtcaat gatgtcagga gaacacacgt tgaaagcggt
    43501 acgaggcagt tttattgatg tcacccgtac ggtcgataac ccggaagaga ttgcctctgc
    43561 gctgcggttt attgaggatg gtttattact cattaaacag ggaaaagtgg aatggtttgg
    43621 cgaatgggaa gacggaaagc atcaaattcc tgacactatt cgcgtgcgcg actatcgcgg
    43681 caaactgata gtaccgggct ttgtcgatac acatatccat tatccgcaaa gtgaaatggt
    43741 gggggcctat ggtgaacaat tgctggagtg gttgaataaa cacaccttcc ctactgaacg
    43801 tcgttatgag gatttagagt acgcccgcga aatgtcggca ttcttcatca agcagctttt
    43861 acgtaacgga accaccacgg cgctggtgtt tggcactgtt catccgcaat ctgttgatgc
    43921 gctgtttgaa gccgccagtc atatcaatat gcgtatgatt gccggtaagg tgatgatgga
    43981 ccgcaacgca ccggattatc tgctcgacac tgccgaaagc agctatcacc aaagcaaaga
    44041 actgattgaa cgctggcaca aaaatggtcg tctgctatat gcgattacgc cacgcttcgc
    44101 cccgacctca tctcctgaac agatggcgat ggcgcaacgc ctgaaagaag aatatccgga
    44161 tacgtgggta catacccatc tctgtgaaaa caaagatgaa attgcctggg tgaaatcgct
    44221 ttatcctgac catgatggtt atctggatgt ttaccatcag tacggcctga ccggtaaaaa
    44281 ctgtgtcttt gctcactgcg tccatctcga agaaaaagag tgggatcgtc tcagcgaaac
    44341 caaatccagc attgctttct gtccgacctc caacctttac ctcggcagcg gcttattcaa
    44401 cttgaaaaaa gcatggcaga agaaagttaa agtgggcatg ggaacggata tcggtgccgg
    44461 aaccactttc aacatgctgc aaacgctgaa cgaagcctac aaagtattgc aattacaagg
    44521 ctatcgcctc tcggcatatg aagcgtttta cctggccacg ctcggcggag cgaaatctct
    44581 gggccttgac gatttgattg gcaacttttt acctggcaaa gaggctgatt tcgtggtgat
    44641 ggaacccacc gccactccgc tacagcagct gcgctatgac aactctgttt ctttagtcga
    44701 caaattgttc gtgatgatga cgttgggcga tgaccgttcg atctaccgca cctacgttga
    44761 tggtcgtctg gtgtacgaac gcaactaata ataaaacttt aacatcctcg tgaggacatc
    44821 attatgtctg gagacatcct acaaacaccg gacgcaccaa agccacaggg cgcgctggat
    44881 aattatttta aaattaccgc tcgtggcagt accgttcgtc aggaagtact ggctggctta
    44941 acgacctttc tggccatggt ttattccgtt atcgtcgttc cgggaatgct gggcaaagca
    45001 ggttttcctc ccgcagctgt gtttgttgcc acctgtctgg tcgcgggctt cggctcgttg
    45061 ctgatggggt tgtgggccaa tttgccaatg gcgattggtt gtgcgatttc cttgacggca
    45121 tttaccgcat tcagtctggt actcgggcaa caaattagcg ttcctgtcgc actgggcgcg
    45181 gtctttctga tgggcgtcat cttcaccgct atttccgtaa ccggtgtgcg tacctggatc
    45241 ttacgtaatt taccgatggg tatcgctcac ggtacaggta tcggtatcgg cctgtttctg
    45301 ctgctgattg ctgctaacgg tgtgggtatg gttatcaaaa acccgattga aggcttgcca
    45361 gtggcgctcg gtgcgtttac ctccttcccg gtgatgatga gcttgctggg gctggcggtc
    45421 atcttcggtc tggagaagtg ccgcgtaccc ggcgggatct tgttggtgat tatcgcaatt
    45481 tcgattatcg gcttaatctt tgacccagcg gtgaaatacc acggtctggt ggcgatgcca
    45541 agtctgactg gcgaagatgg taagtctctg attttcagcc tcgatattat gggcgcactc
    45601 cagccaaccg tacttccgag tgtactggca ttggtgatga ccgcagtgtt cgacgctact
    45661 ggcaccatcc gtgccgtcgc cggtcaggcg aatttgttgg ataaagacaa ccagatcatc
    45721 aacggcggca aagccctgac cagtgactca gtaagttcaa tattctccgg cctggtgggc
    45781 gcagcgcccg cagcggttta tatcgaatca gcggcaggaa ccgccgccgg gggtaaaaca
    45841 gggttaaccg caaccgtagt gggggcgtta ttcctgctga ttctgttctt atcaccgctg
    45901 tcatttttga tccctggtta cgccactgca cccgctctga tgtacgtagg tttgctgatg
    45961 ttaagtaacg tctcgaagct ggatttcaat gattttattg acgctatggc tggcctggtg
    46021 tgtgccgtgt tcatcgttct gacttgtaat atcgttaccg gtattatgct gggctttgtg
    46081 acactggtcg taggccgcgt ctttgcacgc gaatggcaaa agctgaatat tggtacggtg
    46141 atcattactg ccgcactggt cgcattttac gcgggtggtt gggcaatcta atagattctc
    46201 cgcgcctttc agcgcggaga tttctttcac agaggattct caccgctggc aacaatacgc
    46261 tgctggcgga ctcgcctgag ttctctttca gtaagcaact gcagcgcctg tgttgggcaa
    46321 gattcaacgc aggctggccc ctcttcccgc tgttcacaga gatcgcattt cacaatttgt
    46381 tgtcgggtat cccccggcaa cgactgaatg gtgatcattc caaacgggca cgcactgacg
    46441 caactctgac agccaataca acgggcagaa ttggtctgca ccacctgctc gcccatcgtc
    46501 agcgccccca cggggcaagc gccaacacaa ggggcgtttt cacactgatg gcacatcacc
    46561 ggcgcactga tgctgtccag tcgctgtacc ttcagccggg gtaaaaagac atcggcattc
    46621 aactcctgtt ctgaagggtg agcgaccaca caggctactt cacaagtacg gcagccaata
    46681 cagtcagccg gattaacgat aattaacgat ttcatcacga tgccttcgta tcaaacagag
    46741 ttaacatatc gcgcgccgcc tgccttcctg cggccattgc agtgacaacc agatccgcgc
    46801 catgaactgc atcaccacca gcaaagactt ttttcagatg cgtctgggta ggtaaatacc
    46861 cgacgtcacc ggtttgaatc aggccccatt tatcgagttt aattccgctg ccctgcaacc
    46921 acggcatggc atgcgcctgg aaaccaaagg ccataatcag aacatcggcg ggcaattcaa
    46981 actctgaacc cgctaccgga cgaggacgac ggcgaccatc cggccccggc tcacccatgg
    47041 cggtacgaat caggcccacc gcagttaaac gcccatcttc gtcacaagcg atatattgcg
    47101 gttgaacatt gaactgaaac tcgacacctt cctcgcgcgc attaaccacc tctttgcgcg
    47161 agcccggcat actgacttca tcacgacgat acgcgcaggt cacgctggcg gcattgaggc
    47221 ggatggaagt ccgcaaacaa tccattgtcg tatcgccacc gcccaatacc acgactcgct
    47281 taccttccac gtccgtcagc ggatactctt cagactccgg caatcccatg agctggcggg
    47341 tatgggcagt caggaacggt agagcctgaa tgacaccggg cgcatcttca tgcggcagat
    47401 ctgctcgcat catcccgtaa gtccccacgc cgatgaaaac tgcatcatat tcagaagtta
    47461 aatcgctaaa ggtaatatcg cggccaattt cacagttaag atgaaagtcg attcccattg
    47521 cggtgaatat ctctcgccgc tggcttaata ccgttttatc gagtttgaaa ggaggaatgc
    47581 caaaagtcag cataccgcca atttctggat ggcgatcaaa gacatcaacc tgaactcccg
    47641 cgcgcgccag aatatcagca caccctaacc ctgctggccc agcgccaatc accgccactt
    47701 tttcgatacg gggaacaact ttgctgacat cgggacgcca gcccatcgcc agcgcggtat
    47761 cggtgatgta gcgttccaga ttaccgatag agactgcgcc cgagtgatct ttcaaagtac
    47821 atgcgccttc acaaagacgg tcctgtggac ataccctgcc gcaaatttct ggtaaggaac
    47881 tggtctggtg gcaaagttct gccgcttcaa taatctttcc ttcctgtacc agacggatgt
    47941 aatccggaat agcgttatgc agcgggcaat gccagttgca gttagctttt tcggcacaat
    48001 aaacacagcg gtcactctca taagtcgctt gttgtggatc cagcccgcaa tatatttcgc
    48061 caaagtgggt tttccgttca ctcgctgaaa ttttatctgc gcctttacgc gagttaacgg
    48121 ggagcaacgc tgcactgcga gatggctgag cgtctgatga cgcttttcct gctgccgttt
    48181 tgcgctggcg ggccaccttt atctgctgta accctttatc gtccatcagt cgtaacgcct
    48241 gtgttgggca gacttcaata caggcttgcg tgccggaact gcgctggtta caaaggtcgc
    48301 atttctgcgc aatcgtatcg accatctcaa cgacgccaaa ggggcaagcg attgcgcatc
    48361 ttttacagcc aatacatttt tgctcgtcca gttgtacgct atcggactgg aaagtcagag
    48421 cattaaccgg acaagccgta acgcaagggg cattgttgca gtgatggcag gccaccggat
    48481 tcgcagcctg gcctttccct acaacgtgga tacgcggtcg aaagtcactg tgactcagcg
    48541 gccagttttc ttgattgtgt gccaccgcac aggcaatttc acaagcatga cagcctatac
    48601 attccgcagc ttcagcagcg ataaacttat tcatttgcat ccctttcatt taatgagtta
    48661 tgtcttctca aatttcgcga tgcaataaac gggcatatat taggtagcat gaccgtttta
    48721 attataattt tgcgactgag ttcaaattat tgcccctcaa tctatgccgc tttcattatt
    48781 tcaacattgc cgaccaaaag agcgctatcc agataaaaac gattaaatgc gagagtgcga
    48841 catgccagaa tgattaatga aatatcaata tgagaaaatt acagataatt tcattgtcag
    48901 gttctcttct cattttcgtg gtttaaccac gattatgtga ttgcctccgc aattcctgtc
    48961 tctaactccc cttcctcgca aaaactggca ctccacgagc atgtgtttag acagtttcat
    49021 taacgtaaac ggttgctttt tactctggcg ggcgaaagga gaaacactga tgagcgccat
    49081 agattcccaa cttccctcat cttctgggca agaccgccca actgatgagg ttgaccgcat
    49141 attatcacca ggaaagctga tcatactcgg tctgcaacac gtccttgtca tgtacgcagg
    49201 tgcagtcgct gttcctctta tgattggtga ccgactgggc ctctcaaaag aagctattgc
    49261 gatgctcatt agctcggatc tcttttgctg cgggatcgtc acattattgc aatgtatcgg
    49321 tatcggccgc tttatgggga tccgcctgcc ggtgattatg tcggtgacct ttgctgctgt
    49381 aacaccaatg atagccattg ggatgaaccc ggatatcggc ctgctgggga tatttggtgc
    49441 cactatcgcc gcgggtttta tcaccacatt attagcgcca cttatcggtc gcttgatgcc
    49501 tttattcccg ccactggtta ccggtgtggt tattacttct atcgggctta gcatcattca
    49561 ggtgggtatt gactgggccg ccggaggtaa agggaatccg caatatggta atcccgttta
    49621 tttaggtatc tcctttgctg tcttaatttt tatcttgctc attactcgct atgcgaaagg
    49681 atttatgtcc aacgtcgccg tattactggg gattgtattt ggctttttac tttcgtggat
    49741 gatgaatgaa gtcaatttat ccgggctaca tgatgcttca tggtttgcga ttgtcacgcc
    49801 gatgtcattt ggtatgccga ttttcgatcc cgtttccatt ctgaccatga ctgccgtgtt
    49861 aatcatcgtg tttatcgagt cgatggggat gttcctagca ctgggtgaaa tagtcggtcg
    49921 taaactctct tcacacgata ttattcgcgg gctgcgtgtc gatggcgtag ggacaatgat
    49981 aggcggaacg tttaacagct tcccccacac gtcattttca caaaacgttg gcctggttag
    50041 cgtgacgcgc gttcatagcc gctgggtgtg tatttcttcg ggaattatat taatcctgtt
    50101 tggcatggtg ccaaaaatgg cggtgctggt cgcctccatt ccgcaatttg tgctgggcgg
    50161 tgctgggctg gtgatgttcg gcatggtact ggcgacaggg attcgaattc tgtcgcgctg
    50221 taactacacc accaaccgtt acaacctcta tattgtggcg atcagtctcg gcgttggcat
    50281 gactccgacg ctctctcacg atttcttttc taagttaccg gccgtactgc aaccgctgct
    50341 acatagcggc attatgctcg caacccttag cgccgttgtg ctgaacgtct tctttaatgg
    50401 ctatcagcat catgctgacc tggtgaagga atccgtctct gataaagatt taaaagtcag
    50461 gacagtacgt atgtggcttc tgatgcgcaa gctgaagaaa aatgagcatg gagaataata
    50521 tgaatctttt aatgcgcgct atattcagtc tgctgttgct ttttactctc tctattcctg
    50581 tcatttctga ctgtgttgca atggccattg aaagtcgctt caaatatatg atgctacttt
    50641 tttaaatggt ttttacctgt cggcatccgc tcaaaacggg cggttgtcga taaacgctca
    50701 cttggttaat catttcactc ttcaattatc tataatgatg agtgatcaga attacatgtg
    50761 agaaattatg caaacggaac acgtcatttt attgaatgca cagggagttc ccactggtac
    50821 gctggaaaag tatgccgcac acacggcaga caccctctta catctcgcgt tctccagttg
    50881 gctgtttaat gccaaggggc aattattagt tacccgccgc gcactgagca aaaaagcgtg
    50941 gcctggcgtg tggactaact cggtttgtgg gcacccacaa ctgggagaaa gcagcgaaga
    51001 cgcggtgatc cgccgttgcc gttatgagct tggcgtggaa attacgcctc ctgaatctat
    51061 ctatcctgac tttcgctacc gcgccaccga tccgagtggc attgtggaaa atgaagtgtg
    51121 tccggtattt gccgcacgca ccaccagtgc gttacagatc aacgatgatg aagtgatgga
    51181 ttatcaatgg tgtgatttag ctgatgtatt acacggtatt gatgccacgc cgtgggcgtt
    51241 cagtccgtgg atggtgatgc aggcagccaa tagtgaagca agaaaattgt tgtctacttt
    51301 cgcgcagcac aattaaaaaa ccccgacatt tgccggggtt gtgagcataa cgtaatgctt
    51361 attttaccgg acgcatcgcc gggaacagaa taacgtcgcg gatggtatgg ctgttggtga
    51421 acagcattac catacggtcg atgccaatcc ccagacctgc tgtcggcggt aagccatgtt
    51481 ccagtgcggt aacgtaatct tcatcgtaga acatcgcttc gtcgtcacct gcgtctttcg
    51541 cggcaacctg atccaggaag cgttgcgcct gatcttccgc gtcattcagc tcgctaaagc
    51601 cgttaccgat ttcacgacca ccgatgaaga actcaaagcg gtctgtgatt tccgggttaa
    51661 tgtcgttacg acgcgccagc ggagaaactt ctgccggata ttcagtaatg aaggtcggct
    51721 gaatcagatg tgcttctgcc acttcttcga agatctcggt aacgatacgg cccagacccc
    51781 agctcttctc aacgtggatg ccgatagatt cagcaatcgc ttttgcagag tcgaagttgt
    51841 ccagatccgc catgtcggtt tccgggcgat atttcttgat cgcttcacgc atggtcagtt
    51901 tttcgaacgg tttaccgaag tccagcgtca cgtcgccgta ggtcacttcc gtcttaccga
    51961 gaatatcctg tgccagagta cggaacagcg attcggtcag ctcgatcaga tctttgtaat
    52021 ctgcgtaagc catatagagt tccatcatgg tgaactctgg gttatgacgt acggaaatac
    52081 cttcgttacg gaagttacgg ttgatttcga atacacgctc gaagccaccg accaccagac
    52141 gcttgaggta cagttccggc gcgatacgca ggtacatgtc aagatccagc gcgttatgat
    52201 gggtgataaa cggacgcgca gccgcaccgc ctgggatcac ctgcatcatc ggcgtttcaa
    52261 cttccataaa gccgcgattt accatgaact ggcgaatgcc agagaggatc tgcgaacgca
    52321 ctttaaaggt gttgcgggat tcatcgttag agatgagatc cagataacgc tgacgatagc
    52381 gcgcttcctg atcctgcaag ccgtggaatt tgtccggcag cggacgcagt gctttggtca
    52441 gcagacgcag ctcggtgcag tggatagaca gttcgccggt tttggttttg aacagtttac
    52501 ctttcgcgcc gaggatgtcg ccgagatccc attttttgaa ctgctcgttg taaacgcctt
    52561 ccggaagatc gtcacgggca acgtacagct gaatacgacc gccaacgtcc tgcagggtaa
    52621 caaaagaggc tttacccata atacggcggg tcatcatgcg gccagcaacg gcgacttcga
    52681 tgttcagcgc ttccagttct tcgttctctt taccgtcgaa ttctgcgtgc aattggtcag
    52741 aggtatgatc gcgacggaaa tcgttcggga aggcaattcc ctgctcacgc aggttcgcca
    52801 gcttctcacg acgcgttttc agttcattgt taagatcgac taccgcgtca gcgccctgtg
    52861 cgtgttgttc agacatgttg gttcctcata accctgcttt caaacttgct tcgataaatt
    52921 gatccaggct gccgtccagc acggcctgcg tgttgcgggt ttctaccccg gtgcgcagat
    52981 ctttaatgcg ggagtcatca aggacataag aacgaatctg gctgccccag ccgatgtcag
    53041 atttgttatc ttccatcgcc tgtttctcgg catttttctt ctgcatctcc agttcataaa
    53101 gcttcgcttt catctgcttc atggcctgat ctttgttctt gtgctgggaa cggtcgttct
    53161 ggcactgggt cacgatcccg gtcgggatgt gggtaatacg caccgcagat tcggtacggt
    53221 taacgtgctg accgcccgcg ccggacgcgc gataaacgtc aatgcgcaga tccgccgggt
    53281 tgatttcgat atcaatatca tcatcaactt ccggataaac aaacgcagag ctgaacgacg
    53341 tgtggcgacg accgccggag tcaaacgggc ttttacgcac caggcggtga acgccagttt
    53401 ctgtacgcag ccagccgtaa gcgtaatcgc cggagatttt gatcgtcacg gatttaatac
    53461 ccgccacttc accttccgac tcttcgatga tttcagtttt gaaaccacgc gattctgccc
    53521 agcgcagata catacgctca agcatgctcg cccagtcctg tgcttccgta ccgccagacc
    53581 ccgcctgaat atcgaggtag cagtcggcgc tgtcatattc gccagagaac atacggcgga
    53641 actcaagctg cgccagtttt tcttccaggg cgtcgagttc agcaacggct tcgttaaagg
    53701 tttcttcgtc gtcagcttct acagccagtt ccagcagacc agaaacatct tccagcccct
    53761 gtttcatttg gtcgagggtg tcgacaacgg cttcgaggga ggaacgctct ttacccagcg
    53821 cctgtgcgcg ttcgggttcg ttccagacat ccggctgttc cagctcggcg tttacttctt
    53881 ccagacgctc tttcttggcg tcgtagtcaa agataccccc taagaacgtc ggagcgttcc
    53941 gtgaggtcct gaatgcgatt atttaccgga ttaatttcaa acatggtctg atttctttta
    54001 ttgagctagt caaaatgcgg tgataagagc gggattgtac ccaatccacg ctctttttta
    54061 tagagaagat gacgctaaat tggccagata ttgtcgatga taatttgcag gctgcggttg
    54121 ccgcgaaact cgttgatatc gagcttgtaa gccagttgca cttcgcgcac gccgttatcc
    54181 ggccagaggg cggtatcgac attaaaagca ataccatcca gcagtggacc gccgccgacc
    54241 ggttcgacca tcaccttcaa atgacgttcg cccaccagcc gctgttgcag cagacggaaa
    54301 tgaccgtcaa acagcggctc cgggaacatc tgcccccacg ggccagcatc gcgcagcagc
    54361 tgcgccactt ccatggtcat ttcggctgga cttaacggac cgtctgacac cacttcgcct
    54421 tgcaatagcg aagggtccag ccactcagta accagttcgc caaaccgttg ttgaaagagt
    54481 tcgaatttat cctcttccag cgacaaaccc gccgccatcg catgaccgcc aaacttcagc
    54541 atcatgccag ggtagagtgt gtctaatcgc tccagcgcat cacgcatatg cagcccctga
    54601 atggagcgac cggaaccttt cagcgtaccg tcacctgctg gcgcaaaggc gataaccgga
    54661 cggtgaaaac gctctttgat acgcgaagcc agaataccga caacgccctg atgccattcg
    54721 gggtgataca ttgccagccc gccgggtagc gtgtcacggc tgcgctccag tttctcgcac
    54781 agggtcaggg cttcaacctg cattccttgt tcgatctctt ttcgcgtctg gtttagcgca
    54841 tcgagttcat ttgccagcac gcgcgcttcg ccgatgttgt cgcacagcaa cagcgccaca
    54901 ccaacggaca tatcatccag tcgcccggca gcattgagac gcggtcccag cgcaaaacct
    54961 aaatcgctgg cggctagttt ttgtgcatcg cgatttgcca cttccagcag cgctttaatc
    55021 cctggacggc actttccggc acggatgcga ctcatcccct gccaggtcag aatgcgatta
    55081 ttagcgtcca gcggcacgac gtccgctact gtccccagcg ctaccagatc cagcagttct
    55141 gccaggttag gaattgcgat gccgcgctca tcaaaccagc cctgatcgcg caaaaaggtg
    55201 cgcagcgcca gcatcagata aaacgccaca cccacgcctg ccagtgattt cgacgggaaa
    55261 ttacagtcgc gcaagttagg gttaatgatc gcttccgctg cgggtaatgt ttcgcccggc
    55321 aaatggtgat cggtaacaat aaccggaatg cccaacgagc gagcgtgttc aacgcccgca
    55381 tgggaggaaa taccgttatc caccgtgaca attaactgcg cgccacgggc atgggcctga
    55441 tcgactactt ccgggcttaa gccgtaaccg tcttcgaaac ggtttggtac cagatagtcg
    55501 atattgctgc aaccaagcga gcgcatcgcc agcacgctta gagccgtgct ggtggcgccg
    55561 tcggcgtcaa aatcgccgac cacaataatc cgcgttcctt cgcgaaaagc gttgtaaagg
    55621 atctcaacgg ccttttcgac gccgctcagt tgctgccagg gcaacatacc tttaacactg
    55681 cgttccagtt cttgcgcact gcgcacgccc cggctggcgt ataaacggcg cagcaaggga
    55741 ggcaattcag cgggcaagtc tgccgtttca tcgactgcac ggcgacgaag ttgtatctgt
    55801 tgtttcacgc gaattattta ccgctggtca ttttttggtg ttcgtcgagg aattctttca
    55861 tctctttcgg cggctggtaa cccggaacaa gtgtgccatt gctcagcaca actgccggag
    55921 taccgctaac gccaagctgg acgccaagtg cgtaatggtc ggcaatatcc acgtcgcagc
    55981 tggctggtgc gacgctttta cctgccatca catcatcaaa cgctttgttt ttatctttcg
    56041 cacaccagat agctttcatt tctttctctg catcgctgtc cagcccctgg cgcgggaaag
    56101 caagataacg cacggtgatc cccagcgcgt tgtagtctgc catttgctca tgcagtttgt
    56161 ggcagtaacc acaggtaata tcagtaaaca cggtgatgac gtgtttttcc tgcggcgctt
    56221 tataaacgat catctctttt tcaagcgcat tcaactgctt taacagcatc ttattggtga
    56281 cattgaccgg agccgtgcca ctaacgtcat acattggccc ctgaatgata tgtttaccat
    56341 catcggtgat gtacaacacg ccgctgttag tcagaactgt cttcatgcca gctacaggcg
    56401 cgggctgaat atcgctgctt ttgatgccca ttttggctaa cgtttgttga attgccgcgt
    56461 catcagcctg agcaaagcct gaaaacgccg ctaacaaagt aaacaacata aaacctttct
    56521 tcataaatct tcccgttctt ttcagacatc acgcccgcgg gtgatgctgt tgatgaagtt
    56581 gccgcagacg ctcggtagcg acatgcgtat aaatttgcgt ggtggagaga tcgctgtggc
    56641 ccagtagcat ctgcaccacg cgcaaatccg caccatgatt taataagtga gtggcaaaag
    56701 cgtgacgcaa cacatgcggt gacagctttt cgctgtcgat acccgccagc acggcataat
    56761 gtttaatgcg gtgccagaag gtctgtcgcg tcatctgctg cgcacgctgg ctggggaaca
    56821 acacatcaat agagacaccg ttcaacaacc acggtcgccc atgctcgaga tacgtttcca
    56881 gccagtaaac cgcctcttca cctaacggca ccaggcgctc tttgttgcct ttaccaatga
    56941 cccgcactac gccctgacgc aggctgatat cactcattgt cagtccgacc agttcagaga
    57001 cacgcagccc ggtagcatac aacacttcaa gcatggcttt atcgcgtagc tccagtggct
    57061 gatcaattaa tggtgcctgt aataaacgtt cgacctgcgc ttcgcttaaa tcttttggca
    57121 aacgctgtgg caatttcggt gaagcgagat gcgcactggg atcgtcttca cgaaactttt
    57181 cgcgataaag atactggaac aatcggcgca ctgcactcag caaacgcgct gagctggtgg
    57241 ctttataccc gccctccagc cgttctgcca gtaatgcctg caaatcgtca ctttgcgccg
    57301 tcgccagcgt caacccgcgg tgatgcaacc actccaccat cattgacaga tcgcgacggt
    57361 aagcgttcaa cgtattttca gccagatttt tttccagcca cagagcatca agaaactgct
    57421 cgatgcgtgc cagatcctgt ttcacttgcg ccccttatgg tcactcattt gatccattat
    57481 gccttattgt gccgtgacta aagcgattct gatacactag ctgcaaaagc cacagcagaa
    57541 tcgagaagct tacgttatga atatgggtct tttttacggt tccagcacct gttacaccga
    57601 aatggcggca gaaaaaatcc gcgatattat cggcccagaa ctggtgacct tacataacct
    57661 caaggacgac tccccgaaat taatggagca gtacgatgtg ctcattctgg gtatcccgac
    57721 ctgggatttt ggtgaaatcc aggaagactg ggaagccgtc tgggatcagc tcgacgacct
    57781 gaaccttgaa ggtaaaattg ttgcgctgta tgggcttggc gatcaactgg gatacggcga
    57841 gtggttcctc gatgcgctcg gtatgctgca tgacaaactc tcgaccaaag gcgtgaagtt
    57901 cgtcggctac tggccaacgg aaggatatga atttaccagc ccgaaaccgg tgattgctga
    57961 cgggcaactg ttcgtgggtc tggcgctgga tgaaactaac cagtatgacc ttagcgacga
    58021 gcgtattcag agctggtgcg agcaaatcct caacgaaatg gcagagcatt acgcctgatc
    58081 tcactgacgg cttagcgcat atgctttgcc gtcatcttta tctttgcgtc tcttgttgca
    58141 acaaaatccg ccgtaaatcc cgccattcag cttcgtccat gctgtcggct gccagccata
    58201 aatgttgccg tttaccgcca tcagaacgta aacgcagcat catgccgctc ttaatcatcc
    58261 acggtgcttt gacgatgctc cactcctgcc cttgccaacg caaacgcccg tccatcaaca
    58321 agcgaatttc cccctggcga gcattaatac gccgctggct gcgaacgcaa tcaaacacca
    58381 ccagcgaaag taacaccatc cataacgggg tgtaactgag tggccagggc atgagtaaaa
    58441 taacagcggc aaccagccca tgaatcagca aggaaagcca ctgtgcgcgc caggagacgc
    58501 gcaaatcaga ttgccacagg accacgttcc cggttccgtg tctggatgag tcggaccatc
    58561 atttccagtt ctgcatcggc cggtttaccg tgattcatca gccagttaaa caggtccgga
    58621 tcgtcacatt ccagcagacg aataaagatg cgtttttcgt catcgcttaa gctgtcgtac
    58681 tcatgttcga aaaacggcat gattgaaata tcgagttcgc gcataccacg gcggcatgcc
    58741 caatgaatgc gggctttgtt gttaatgtcc atcttcttcc tgtctcacga aaatccagta
    58801 ccccgctatt gtaacgtgtt tttcgccttc ttttacggga atatcagtaa acaccatcgc
    58861 gatcgcgaaa ttaattcaca acaattcaat ggcttcattt ttttggaagt cgcctcgcag
    58921 aaggcacaga tcgcgtagtg aaagcacttg cattgcctca tagctctttt accattagtc
    58981 attaatacgc cgttaagcaa ctcaggactc tattatggct tttacacctt ttcctccccg
    59041 tcagcctacg gcttctgccc gtttaccgct gacgctgatg acgcttgatg actgggcgct
    59101 tgccaccatt actggcgcgg acagcgaaaa atatatgcag ggtcaggtga cagcagatgt
    59161 cagccagatg gcagaagatc agcacctgct cgccgcccat tgcgacgcca aaggtaaaat
    59221 gtggagcaat ttacgtctgt tccgcgacgg cgatggcttt gcatggattg aacggcgcag
    59281 cgtgcgtgaa ccgcagctga ctgaactgaa aaaatatgcg gtattctcta aagtgaccat
    59341 cgcgccagac gacgagcgtg tgctgcttgg tgttgccggt tttcaggcgc gcgccgcgct
    59401 ggcaaatctc tttagcgaac tgccttcgaa agaaaaacag gtagtcaaag aaggcgcgac
    59461 cactttgcta tggtttgaac acccggcaga acgtttcctg atcgtaaccg atgaagctac
    59521 tgctaatatg ctgaccgata aactgcgcgg tgaagcggaa ctgaacaata gccaacagtg
    59581 gctggcatta aacattgaag cgggtttccc ggtgattgat gccgccaaca gcgggcagtt
    59641 tatcccacag gcgaccaatc tccaggcgct gggcggtatc agctttaaga aaggctgtta
    59701 taccggacaa gagatggtgg cgcgagcaaa attccgtggt gccaacaaac gcgcgctctg
    59761 gttgctggca ggtagcgcca gccgactgcc ggaagctggt gaagacttag ggctgaaaat
    59821 gggcgagaac tggcgtcgta ccggtacggt gctggctgcg gtaaaactgg aagatggtca
    59881 ggtcgtggta caggtcgtca tgaataacga tatggaaccg gacagcatct tccgcgtacg
    59941 cgacgatgcg aatacactgc atatcgagcc gctgccgtat tcgctcgaag agtaaatctc
    60001 tgtatcgcat caggcattta tcgcctgatg cgacgctgac gcgtcttatc atgtctggga
    60061 tttgatgcct ttatcacaac agggacgagg cccgtagatc ggataaggcg ttcacgccgc
    60121 atccggcggt tgcgctccga tgcctgatgc gacgctggcg cgtcttatca ggcctacaaa
    60181 ggcataccca ttacgcctgc ccaatataca aatagatcgc cagaaagtgg cacacactac
    60241 cgccgagcac gaagccgtgc cagatggcat ggttgtatgg aatgcgtttg cagacgtaga
    60301 aaatcacccc gagcgaataa accacgccgc ctaccgccag taaggtaacg ctgcccgccg
    60361 cgagcttaac tgccatttca taaattacca ccagcgacag ccagcccatc gccagatagg
    60421 tcaccagaga taaaattttg aatcggtgcg cgatggtcag tttaaacaga atacccagca
    60481 atgccaggct ccagataaca atcatcaacc cgcgcgctaa cggagaatcc agccccacca
    60541 gcaaaaacgg cgtgtaggtt ccggcaatca acaggtaaat agcgcaatgg tcaaatttct
    60601 tcagccacat ttttgcccgt tgatgaggaa tggcgtgata gagcgtcgaa gcgaggaaca
    60661 gcaggatcat actgccgcca tagaggctgt agctggttat cgctgtggcg ctggcattga
    60721 gatccaccgc ctgaaccagt agcaacacca gcccaacgat accaaacacc aacccaatgc
    60781 cgtgactgac gctgttggca atttcctctg ccagcgaata tccctgctta atgaggggct
    60841 tctgaaccat aacttactcc ggagaaacgt acacgcacat gtatacctct ccagcgtaac
    60901 tgagaatagt tccagtgaac acctgttagc taaaataaat tctgatttga gactaatctc
    60961 ctaaaaatca tgaaattaaa tgagaaattt caactaacag gcgtgagttc aatttaaaga
    61021 catttaaatt caatcacata aaactgtgtc tgaccgggat agatgtcggc aatgaccttt
    61081 ttcagttcag tcagggtcat attttcctgc tctgcatgtt tttcagtcag cgtatccagc
    61141 cttacggttg aggttgcggt gacttcaatc gtgcaaaaat aaccgtcatc ttcaaaacgc
    61201 ccgacacgaa gcacatcacc cgttttgaag tgcgattcag actcgtcgcg gatggtgatg
    61261 gttttacgcc cagccagaat gtcatcctgg aaacgttgaa aaaaagtgat gtcgtttggc
    61321 tgcatggtac tatttcctgt aagaattgac tcatctggag cctatgatag tgaaaaaact
    61381 caccttaccg aaagatttct tatggggcgg cgcagttgcc gctcatcagg tcgaaggcag
    61441 ctggaacaaa ggcggcaaag agccgagcat ttgtgacgtt ctgaccggtg gcgcacacgg
    61501 cgtgccgcgc gaaatcacca aagaagtctt gccaggcaaa tactatccaa accatgaagc
    61561 cgttgatttt tatggtcact acaaggaaga catcaagcta tttgccgaaa tgggcttcaa
    61621 atgttttcgg acttccatcg cctggacgcg tatttttcca aaaggtgatg aagctcagcc
    61681 aaacgaagaa gggctgaagt tctacgatga tatgttcgat gaactgctga aatacaacat
    61741 cgaaccggtg atcaccctct cccactttga aatgccgctg catctggtgc agcaatacgg
    61801 tagctggacc aaccgtaaag tggttgattt ctttgtccgt ttcgcggaag tggtgtttga
    61861 acgctataag cataaagtca aatactggat gaccttcaac gaaattaaca accagcgtaa
    61921 ctggcgtgca ccgctgttcg gttactgctg ctccggcgtg gtttataccg agcatgacaa
    61981 cccggaagag acgatgtacc aggtgctgca tcaccagttt gtcgccagcg ccctggcggt
    62041 gaaagctgcg cgtcgcatta acccggagat gaaagtcggc tgtatgctgg cgatggtgcc
    62101 gctctatccc tactgctgta acccggacga tgtgatgttc gctcaggagt cgatgcgcga
    62161 acgctacgtc tttaccgatg tgcagctacg cggctattac ccgtcctatg tgttgaacga
    62221 gtgggagcgt cgcggattta acatcaaaat ggaagacggc gatctcgatg tactgcgtga
    62281 aggcacctgc gattatcttg gtttcagcta ttacatgacc aacgcagtga aggccgaagg
    62341 cggcaccggc gatgcgatct ctggtttcga aggcagcgta ccgaacccgt atgttaaagc
    62401 gtctgactgg ggctggcaga ttgatccagt aggtctgcgc tatgcccttt gcgaactgta
    62461 tgagcgttat cagaggccgc tgtttattgt cgaaaacggt tttggcgctt acgacaaagt
    62521 ggaagaagat ggcagcatca acgacgacta ccgcattgac tacctgcgcg cccatatcga
    62581 agagatgaaa aaagcggtga cttacgatgg cgtggatctg atgggctaca caccgtgggg
    62641 ctgcatcgac tgcgtatcgt tcaccaccgg gcagtacagc aaacgctacg gctttatcta
    62701 tgtgaataaa catgacgacg gtgctggcga tatgtcgcgt tcacgtaaga agagctttaa
    62761 ctggtaccaa gaggtgattg ccagcaacgg cgagaagctt taagtcgatg aagtaccgga
    62821 tgcaatactt gttgcatccg gtcagacaac ttccctgttt tatttcccgc ccgccaaatc
    62881 gataaaactt cccgtgacgt aagaggcttt atcacttagt agccagacaa tggcctgcgc
    62941 gacctcttct gcctgtccac cacgctgcat ggggatgttc gacttaacgc gatcgacgcg
    63001 tccaggctcg ccgccgctgg cgtgcatttc ggtataaata aaccctggcc gcacgcagtt
    63061 aacgcggatc ccctgcgcgg cgacttccag cgatagtccg gtggttaacg tatcaatcgc
    63121 ccctttcgat gccgcgtaat caacatattc ccctggcgaa cccaaccgtg aggccaccga
    63181 agagaaattg acgatagcgc cgccactgcc accattttta agcgccatgc gttttaccgc
    63241 ctcgcggcag cagagaaaat atcccgtcac gttggtggaa agtactcggt tgattcgctc
    63301 tgcggtaagg ttttcaacgg tgcactgggt aaacaagatc ccggcgttat tgaccagcgc
    63361 tgctagcggt tcatcgtgct gatcgattgc tgtaaacatc gcaacgacct ggttttcgtc
    63421 gctgatatcc gcctggagca cgaatgcttt gccaccggct tgcgttatta agttcatcac
    63481 ttcctgcgcc gcgtggaggt tttgctgata attaaccgcc accgtatacc cttcttgcgc
    63541 caacagtaat gcagttgccc gcccgatgcc gcgactgcca ccggtcacaa gtgctataac
    63601 catttgtttt tccgagaaaa attcattcgt tgacgtatct ttgagattgc taacaaactg
    63661 gctcaatctt caatgttggc acgcttttcc ccctcgccct ttcagggaga gggccggggt
    63721 gagggtaaat attcgcgcca gtgccggcct gttcccctca ccctctcccc aaaggggcga
    63781 ggggactgtc tgagcacttt tgtactttgt catctgacta aaaaggcgcc gaagcgcctt
    63841 tagaaaatag tcgaatcagt gaattactgg tattcgctaa tcggtacgca ggagcagaac
    63901 aggttacggt cgccgtaaac atcatccaga cgtttcaccg tcggccagta tttgtctgcc
    63961 acacctgccg ggaatactgc aacctcacgg ctgtacggat gcgcccactc ggcgaccagt
    64021 tcgttctgaa tgtgcggcgc gttcaccagc gggttatctt ccagcggcca gacaccggct
    64081 ttaacctggt cgatttctgc gcggatagcc agcatcgcgt cgataaagcg atccagttcc
    64141 actttgcttt cagattcagt cggttcaacc atcagcgtac ccgccaccgg gaacgacatg
    64201 gtcggcgcgt ggaaaccgta gtcgatcagg cgcttggcaa tatccagctc gctgatgccg
    64261 gtttcttctt tcagcgggcg aatatcgaga atacattcgt gcgccacgcg accgtcgcga
    64321 ccggtataca gcaccgggaa ggcatcctgc aggcggctgg caatatagtt ggcgttgagg
    64381 attgccacct ggcttgcttt tttcagccct tctgcgccca tcatgcggat gtacatccag
    64441 ctgattggca ggatagaggc gctaccgaac ggtgccgcag aaactgcgcc ctgacgggtt
    64501 aacatgcctt cgatttgcac cacgctatga cccggtacaa acggtgccag atgcgctttc
    64561 acaccgatcg gtcccatacc cggaccacca ccgccgtgcg gaatgcagaa agttttatgc
    64621 aggttgaggt gcgaaacgtc cgcgccaata aagcccggcg aggtgatgcc aacctgggcg
    64681 ttcatgttcg cgccatcaag gtaaacctga ccgccgaact gatgcacgac ttcacacact
    64741 tcacggatcg tttcttcata cacgccgtgg gtagaaggat aagtcaccat gatacaggag
    64801 aggttatcgc ccgcctgttc cgctttcgcg cgcagatcag tcagatcgat gttgccgttt
    64861 ttatcacacg ccacaaccac cacctgcatt cctgccatat gtgcagaagc ggggttagtt
    64921 ccgtgcgcag aagccgggat caggcagata tcgcgatgcc cttcgttgcg gctttcatga
    64981 taatgacgaa tcgccagcag gcccgcgtat tcgccctgtg cgccggagtt cggctgcata
    65041 caaacggcgt cgtaaccggt cagtttcacc agccagtcag ccagctgcgc aatcatctgc
    65101 tgataacctt cagcctgttc cggcggacaa aacgggtgca gttcggcaaa ttccggccag
    65161 gtgattggga tcatctcggc ggcggcgttc agtttcatgg tgcaggaacc cagcgggatc
    65221 atcgcctgat tcagcgccag atctttacgc tccagcgagt gcatatagcg catcatttcg
    65281 gtttcgctgt ggtagcgatt aaacaccgga tgggtgagga tttcgtcgtc gcgcagcatc
    65341 gcaggctgga tagagcggct gtcgtgagcc acgtctttgt ccagcgtgtc gatgtccagg
    65401 ccgtggttat cgcccagcag cacgttgaaa agctgcatta cgttttcacg cgtggttgtt
    65461 tcatcaaggg tgatcccaac cgcgttcaga atatcgctac gcaggttgat ttcagccgct
    65521 tcggcacgcg tcagtacgcc cgctttgtcg gccacttcca cacacaaggt gtcgaaatag
    65581 tgcgcatggc gcagtttcag acctttttgt tgcaggcccg ccgccaggat atcggtcaga
    65641 cggtgaatgc ggttagcgat acgtttcagg ccaatcgggc cgtgataaac ggcatacagg
    65701 ctggcgatgt ttgccagcag cacctgggaa gtacaaatgt tggagttcgc tttctcacgg
    65761 cgaatatgtt gctcgcgagt ctgcatcgcc atgcgcagcg cggtattgcc agctgcatct
    65821 ttcgatacac cgataatacg gcccggcatt gagcgtttgt attcatcttt cgccgcaaag
    65881 aaggccgcgt gtgggccacc gtagcccatc ggcacgccga agcgttgcgc cgaaccaaaa
    65941 acaatatccg cgccctgttt acccggcgca gttaacagca ccagcgccat aatatcggcg
    66001 gcgacactga ccacaatttt gcgtgatttc agttcgctaa taagcgcggt gtagtcgtgg
    66061 atttcaccgg ttgtgccaac ctgctgtaac agcacgccga agacgtcctg atggtcgagc
    66121 actttttgcg cgtcatcgac aatcacttca aaaccaaagg tttcggcacg agtacggacc
    66181 acatccagcg tttgcggatg cacatcggaa gccacgaaga agcggttggc atttttcagt
    66241 ttgctgacgc gtttcgccat cgccattgct tcggcggcag cggtggcctc gtccagaaga
    66301 gaagcagagg ccatatccag tccagtcaaa tccagcgtta cctgctggaa gttgagcagt
    66361 gcttcaaggc ggccctggga gacttcaggt tgatacggag tgtacgcggt ataccagccc
    66421 ggattttcca gcatgttacg caggataacc ggcggtagct gcacggcggt gtaacccatg
    66481 ccgatgtaag acgtgaagcg tttattgcga ctggcaatag ccttgagttc tgccagtgcg
    66541 gcgtattcgg tcgccggtgc gccaacctgc ggcggggtcg caagctgaat atctttcggc
    66601 acaatctggc cggtcagcgc gtttaacgat tgtgcaccaa cggcattcag catttcttgc
    66661 tgttgcgcgg cgtccggtcc gatatggcgt tcaataaaag cgccgctgtt ttcaagctgg
    66721 cttaacgtct gtgtcatgag cgatggttcc tgaaacgtgc agtgaattgt gaacctctct
    66781 ccttacgaag agagtgaggg tgaggcataa attttcctca ccctgatcct ctcccgcaga
    66841 agaggaataa agccgttact cgtcttctaa caatgcttcg tatgcggtcg catccagcag
    66901 tgattccagt tcgctttcat cgctggcttt gattttaaag atccagccac ctgcatacgg
    66961 ttcgctgttc accagttccg gggaatcgct cagtgcgtca tttaccgcca cgatttcacc
    67021 gcttactggc gcataaatgt ctgacgccgc ttttactgat tcggcaaccg cgcagtcatc
    67081 gcccgcgcta accgttgcgc ccacttccgg caggtcaaca aacaccatat cgcctaacag
    67141 ctcctgggca tgttcggtta taccaacggt gtaagtgccg tcggcttctt tacgcagcca
    67201 ttcgtgttct ttgctgtatt tcagttctgc tggtacgttg ctcatcaatc aatctccaaa
    67261 aaagtaaatc acgcgacggc tttaccgtta cgcacaaaaa caggtttcgt cactttaacc
    67321 ggcatttcac ggttgcgaat ttgcacaatc gccgtttcgc caataccttc cggcacgcgt
    67381 gccagcgcaa tgctgtaacc cagcgtcggg gagaaagtcc cgctggtgat aatgccttca
    67441 tgctggttgc cctgcgcatc ggtaaaacgt accggcagtt cattacgcag cacgcctttt
    67501 tcggtcatca ccagaccaac cagtttttct gtaccatgct cacgctgcac ttccagggct
    67561 tcacgaccga taaagtcacg atctgccggt tcccaggcga tagtccagcc catgttggcg
    67621 gctaaaggag aaatagtttc gtccatctcc tgaccataaa gattcatgcc cgcttccaga
    67681 cgcagcgtgt cacgcgcgcc caagccacat ggcttaacac ccgcttccac cagcgcacgc
    67741 cagaaatcgg ccgctttttc attgggcagc gcaatttcat agcccgcttc accggtataa
    67801 ccagtggtgg caataaacag atcgcccgcc tgcacgccaa agaacggttt catcccttcc
    67861 accgcctgac gctgggcgtc attaaacagt gtggcagctt ttgcctgcgc attcggccct
    67921 tgcacggcaa tcatggaaag gtcatcacga acggtaattt cgatgccgaa aggttcagcg
    67981 tgttgggtaa tccaggagag gtctttttcg cgggtggcgg agttaacaac gaggcggaag
    68041 aaatcttcag taaagtagta gacgatgagg tcatctatca caccgccaga ggcattcaac
    68101 atccccgagt aaagggcttt gccgcttttg gtgagcttcg ccacatcgtt cgccagcaga
    68161 taacgcagaa actcccgggt gcggctgccg cggagatcga cgatggtcat atgtgacaca
    68221 tcaaacattc cggcatcggt acgtaccgca tgatgttcgt cgatttgcga accgtaatgc
    68281 agcggcatca tccagccgtg gaaatccacc atgcgagcgc cgcaaagcgt gtgttgttcg
    68341 tacaaaggag tctgttgtgc catcttgtcc tcattgaata agcggggctg acaacttttc
    68401 atggtgaaat tatcaccacg aaacccagca tcggagccac tcccggtccc caacgcaatc
    68461 gttctctttt gcctgaactt accaccgaaa cagactgtta accataaggt aaaattgatc
    68521 atcgcattag cttatggtta aaaaacgcca aaatcgcgac agaataaaaa accaaaaaac
    68581 acaccaattt ctatataaag atgatgccat tagaaagcta atttgaataa ggcaatatta
    68641 agagctaaaa aaatgtcaaa aaacactaaa tcaaaaaata atgacattag aaaatataat
    68701 gcgaaaacgg aggtgaaatt agtttatttc aaatgaggaa aatctcccgg cgaaaaaacc
    68761 gggagatgaa agtgtgatgg gtatcaaata aacagcagaa gagaaatttt taacgcagcc
    68821 attcaggcaa atcgtttaat cccattgcct ggcggataag ttgtggttta acgccaggaa
    68881 gcgtgtcggc cagtttcaaa ccaatatcac gcagcagttt tttcgccgga ttggtaccgg
    68941 aaaacagatc gcggaatccc tgcataccag ccagcatcag cgccgcactg tgcttgcggc
    69001 tacgctcata ccggcgcagg taaatgtact gcccgatgtc tttcccctga cgatgcaacc
    69061 gtttcagttc agcaatcagc tctgcagcat ccataaagcc gagatttact ccctgccccg
    69121 ccagcgggtg aatggtatgc gcggcgtcgc ccaccagcac cagacagtgc gcggcaaact
    69181 ggcgcgcata acgccccgtc agtgggaaca cctgacgcgc gctctcaacc ttgcataagc
    69241 ccaggcgatt atcaaaagcg atatttaacg cgcgattaaa ttcatcttca cttgcctgct
    69301 gcatccgctg cgcttcctct ggcgacagtg accagacaat tgagcaaaga tgcggatcgc
    69361 taagcggtaa aaaggccaga atgccttcgc catggaaaac ctgccgcgcc accgcatcat
    69421 gcggttcttc cgtgcgaatg gtcgctacca gcgcgtgatg ctgataatcc cagaaagtca
    69481 gcggaatatc tgctttgttg cgcaaccagg aattagcacc gtccgcgcca atcaccagac
    69541 gcgccgttaa catgctgcca tctttcagcg tcaggaaagt ttcattttct ccccaggcga
    69601 cctgctgtaa ttctgcgggg gccaacagag tgatatctga cgactgctgc gctttgttcc
    69661 acaacgcgta gtgaatcact gagttttcaa cgatatgccc aagatggcta tagcccatgc
    69721 tttgatcgtc aaacgaaatg tgaccaaaac tgtctttgtc ccacacttcc ataccgtgat
    69781 aacaactggc cctacgagag agaatgtcct gccagacgcc aagacgggtg agtaattttt
    69841 cgctggcggc attgatagcc gaaacgcgca gttgtggtgg tgcatccgcc gccagaggtt
    69901 ccggtacgcg ctgctccagt acggcaacgc gtaagccgct cccctgtaag ccgcaggcaa
    69961 ccgccagccc caccatgccg ccaccaacaa tggctacatc aacactttgc attgtttatt
    70021 ccttaaaacc gccttcaacg cgccacccaa ccgagggtgc gctgcgccag cacatcgcgt
    70081 gccggggtga ataattccat cgtcatcagt ccgatgttgc gcccgacaac cagcggtgcc
    70141 caacggttgg caaaaagatg taccaggctg tccgtgacgc cgatggttgc ttcgcgatcg
    70201 ctctgtcgac gctgttgata gcggcacaat atgccgtaat caccgatgtc ttctccgcgc
    70261 tcatgcgcct gagttagggt ttccgcaagg ctcatgacat ctcgcatacc gaggttaaac
    70321 ccttgcccgg caatcgggtg cagagtttgc gccgcattgc ccaccagcac ggtacgatgg
    70381 gtaatagctc tggcggcgcg ggttaacgcc agcggataag cactgcgttt accggcgtgg
    70441 gtaatttttc caagtcgcca gccaaaggcc gactggagtt cacggcaaaa cttctcgtcg
    70501 ctccacgaca gcacctcttc gcgccgttcc agtggatgac accagaccag cgaacagcgt
    70561 ccgtcagaca tcggcaacat cgccagcggg ccatgttgcg tgaagcgttc aaaagcgcgg
    70621 ccttcgtgcg caacggaagt cgcaacgttg gcaatcacgg ccagttgttc gtaaggctcc
    70681 tgctgccaat caacgccgca cgcggtggct aacgctgaat gggtgccatc agcggctatc
    70741 agcacgcggc ccgtcagcat ctcgccgctc tccagcgtca cttcaacgtg actctgagta
    70801 cgggcaacgt tagctacgcg atcagggcaa tgcagcgtta cgccaggtgc ttttcgcagc
    70861 aatgcaaaca gccgttgccc gacattatgc aattcgacaa cctgccccag cgccgccagt
    70921 tggtaatctt ctgcggcgag ggtgacgaat ccagcatgac cacgatcgct gacatgcacg
    70981 gtagtgatgg cggttgcgca atcagccaga gattgccaga cgccgatgcg cgccagttgc
    71041 tgacaggtcc ccgccgctag cgcaatagct cgtccatcaa agcccggatg agcatgtgac
    71101 tctggcgcgg tcgcttcaat caaatgtacc ggcagcgccc cgtgacttaa ccgggaaata
    71161 gccagcgcca gcgtcgcgcc cgccatgccg ccaccgacga tgattacgct cattgctttc
    71221 tcgcagctgc catcaacgct tcgatttctt ccggcttttt caccacgctg gcggtgaggt
    71281 tttcgttacc ggtttcggta atcacaatgt cgtcttcaat acgaatgccg ataccgtgat
    71341 attgttctgg cacttctgca tccggcgcaa tatacagccc tggctctacg gtcagtacca
    71401 tgcccggttc cagaatgcgc gagcgatcct gaccataaac gccaacgtca tggacatcta
    71461 gccctaacca gtggctaagg ccatgcataa agaaaggacg atgggcgttc tgagcgatca
    71521 gttcatcaac atcacctttc agaatgccga gttttaccag gccgctaacc atgatgcgca
    71581 ccacttcacc ggtgacttcc tgcatggaag ttcccggacg atacaggcgc aagctggttt
    71641 cgagagactc cagcacaatg tcgtagattt cacgctgggc ctgagtgaat ttgccgttga
    71701 ccgggaaggt gcgggtaata tcgccagcgt aacctttgta ttcacaaccc gcgtcaatca
    71761 acaccaggtc accgtcacgc agttcacact cgttttcggt gtagtgcaaa atgcagccgt
    71821 tttcaccgct gccgacaatg gtgttatagg aaggatagcg cgcgccgtgg cggttaaatt
    71881 cgtggtgaat ttcgccttcc aggtggtact cgaacattcc cggacggcat ttttccatcg
    71941 cccgggtatg cgccagggcg gtgatttcac ccgcgcggcg aagtacggca atctcttctg
    72001 gcgatttgaa caggcgcatt tcatgaacaa tgggacgcca gtcgatcatc gttgccggcg
    72061 cggtgagatt ttgtcgcgaa cctttacgca gcttttccag cgcgctgttc acgatttcat
    72121 cagcataggt atattcgccc tgggcgtggt aaaccacatc cagaccatta agtaattgat
    72181 aaagttgctg attaatttcg ctgaatgcca gtgcgcggtc aacgcccagt ttctctggcg
    72241 cggcatcctg acctaaacga cggccaaacc agatctccgc cgtcaggtcg cgaacgcggt
    72301 taaacagaac gctgtggtta tgagtgtcat cgcttttaat cagcaccagc accgcttccg
    72361 gttcgttaaa gccggtgaag taccagaagt cactgttctg acgatagggg tattcgctgt
    72421 cggcgctacg tgttacttct ggtgcagcaa aaatcagcgc ggcgctgccg ggttgcattt
    72481 gctccaccag ggcctgacgg cgacgctgaa actcttgccg ggatatctca ctcataacac
    72541 tctccttacg ttttttgttt ttagtgtaga gtcggttttt gtacttctgg cgcggtcggt
    72601 tgcggatgag taaaggtgtc gtggcataac agcgcggcaa cacgaacgta ttcgatgatc
    72661 tcttcaagcg acatttcaag ctcttcctga tcttcgtctt cgtcgtaacc cagttgcgca
    72721 atgttacgca gatcgtcgat agcttcaccg gtttcgccgg tcactttatc cagcttcggt
    72781 tgcgtaacgc caagaccaag caggaagtga ttgacccaac ctgccaatgc atcagcccga
    72841 tcgaaaacgc tgacatcatc gccatcaggc agataaagct gaaaaaggaa gccgtcatcc
    72901 tgcagggcat cgctggtggc agagtgcatt ttacgcagtg cctgtgccag ctcatgaccg
    72961 aaagccatgc cttcgttcgt caggtcgtga agtagcggta gccatgagct gtcatcgtta
    73021 ccgccacata tcatcccact gattaaacca tgcatctcag caggggtcag acccgtccct
    73081 tgttggttca gatactggtt catttcgttg taaccaggca tttcgttctg tatagacata
    73141 agcattcgtc gtcaaaggga ggaatattca tgatatgcta ccactttggg ccctggtgga
    73201 ccagaaaagg gcttgtctct tctcatcagg gtagctatag tgtcgcccct tcgcagacca
    73261 tgggtctaaa gacgaaggca gcgcagtcaa tcagcaggaa ggtggcatgt ctgcacaacc
    73321 cgtcgatatc caaatttttg gccgttcact gcgtgtgaac tgcccgcctg accaaaggga
    73381 tgcgttgaat caggcagcgg acgatctgaa ccaacggttg caagatctga aagaacgcac
    73441 tagagtcaca aatactgaac agttggtctt cattgccgca ttgaatatca gctatgagtt
    73501 agcgcaagaa aaagcgaaga ctcgtgacta cgcggcaagt atggaacagc gtattcggat
    73561 gctgcagcag accatagaac aagcgttact tgaacaaggt cgcatcaccg aaaaaactaa
    73621 ccaaaacttt gaatgacact tttcggttta ctgtggtaga gtaaccgtga agacaaaatt
    73681 tctctgagat gttcgcaagc gggccagtcc cctgagccga tatttcatac cacaagaatg
    73741 tggcgctccg cggttggtga gcatgctcgg tccgtccgag aagccttaaa actgcgacga
    73801 cacattcacc ttgaaccaag ggttcaaggg ttacagcctg cggcggcatc tcggagattc
    73861 ccttcttatc tggcaccagc catgacgcaa ctaccagaac tcccactgac attatcccga
    73921 caagaaatcc gcaaaatgat tcggcaacgt cgtcgtgcgt taacgccgga acaacagcag
    73981 gaaatgggtc aacaagccgc tacccggatg atgacttatc ccccggtggt gatggcgcat
    74041 acggtcgctg tattcctctc ttttgatggt gaactcgaca cccagccgct aatagaacaa
    74101 ctctggcgcg ccggtaagcg cgtatatctt ccagttttgc atccctttag tgccggtaat
    74161 ttgctgttcc tgaattacca tccgcaaagc gaactggtga tgaacaggtt gaagatccat
    74221 gagccaaaac tggatgtgcg tgacgtgcta cccctttccc gattagacgt gctgatcaca
    74281 ccgctggtcg cctttgatga gtacggtcag cgcctgggaa tgggcggtgg tttttatgat
    74341 cggaccttac aaaactggca gcactataaa acgcaaccgg tgggttatgc gcatgattgt
    74401 cagttggtgg aaaaactccc cgttgaagag tgggatatcc ctcttcctgc ggtggttaca
    74461 ccgtcgaaag tctgggagtg gtaagggcga tacacctgca tcgccctgat tgacatcgtt
    74521 gattctttaa cctaatttag tgagtaaggg taagggagga ttgctcctcc cctgtgactg
    74581 actgttaata agctgggaaa cttatgagta acagtacaat cagtatgatg gcaagtcgca
    74641 tcataaccct tctccttcaa gccctcgctt cggtgagggc tttaccgtta cagccccatg
    74701 ctgcccagct atcgtaagct ctctttaaat aaccacaacg cattgatcta cctttaaatc
    74761 ttttcttcga gcgtgctcga aaaaccccat gcaaaacaga gataattttt gggaaaaagg
    74821 atctattacg gcagtaaacg tgggcgatga agtggctatc aaaaagttcg gttcagggcg
    74881 atgcaccccg catcgccctg attgacatcg ttgattcttt gacctaattt agtgagtaag
    74941 ggtaagggag gatgactcct cccctgagac tgactgttaa taagctccga aacttatcag
    75001 taacagtaca atcagtatga tgacaagtcg catcataacc cttctccttt aagccctcgc
    75061 ttcggtgagg gctttaccgt tacagcccca tgctgtcccg ccatcgtaaa tccccattaa
    75121 ataaacacaa cgcattgatc tgactttgat ttattttctg gagcagactc gcaaagtaga
    75181 atgcgcaacg cggcaacggt gtggagaagg gataaaaaaa cgggcaagtc agtgacctgc
    75241 ccgttaattt tcagagaagg ggaattagta cagcagacgg gcgcgaatgg tacccggaat
    75301 agctttcatt gcctgcagcg ctttttcggc aacgtcttcg tcagcttcaa tatcaataac
    75361 tacataaccc atctgggcgg aagtttgtaa atattgcgcg gcgatgttga cgccctgctc
    75421 ggcgaagatt ttgttcagcg cagttagcac gcccggacgg ttttcgtgga tgtgcatcag
    75481 acgacgacca ccatgcagtg gcagcgagac ttccgggaag ttcaccgcag agagcgttga
    75541 gccattgtca gaatacttga tcaatttacc cgcaacttcc aggccgatat tctcctgcgc
    75601 ttcctgagtc gaaccgccaa tgtgtggcgt cagaaggacg ttgtcgaatt cacacagcgg
    75661 agaggtaaat ggatcgctat tggtcgccgg ttccgtcggg aatacgtcga ttgccgcccc
    75721 cgccagatgt ttgctcgcca gcgcatcaca cagcgccgga atatccacca cagtaccgcg
    75781 cgaagcatta atcagcagcg agccgggctt cattagtgaa atttctttcg cgcccatcat
    75841 atttttggtg gacggattct ctggtacatg cagactcacc acatcgctca tattcagcag
    75901 gtcagaaaga tgctgtacct gagtggcgtt gcccagcggc agtttatttt caatatcata
    75961 aaagtaaaca tacattccca gcgattcagc cagaatgccc aattgcgtac caatatgacc
    76021 gtagccgatg atacccagct ttttgccgcg cgcttcaaaa gaacccgccg ccagtttgtt
    76081 ccacacgcca cggtgcgctt tagcattggc ttccggcacg ccgcgcaata gcagcagcag
    76141 ttcgccaatc accagctccg caacagagcg cgtatttgag aacggtgcgt taaataccgg
    76201 gatcccgcgc tttgccgccg catccagatc aacctggttt gttccgatac agaaacagcc
    76261 aatagcgacc agtttttctg cggcgttgat cacgtcttca gtcagatggg tacgggatcg
    76321 caggccgatg aagtgggcat cgcggatgga ttcttttaat tgttcatcat ccagcgcgcc
    76381 tttgtgaaat tcgatgttgg tgtaaccagc tgcacgaagg ctttccagcg ccttttggtg
    76441 cacgccttct accagcagaa acttaatctt gtctttctcc agcgatacct ttgccattta
    76501 cccaatcctg tcttttgaaa tgttgtgtgc ggatttgcgt ccgcctttca acatatcaaa
    76561 aaataatatt gcggcaatat gaacgtttgc gtcgcgatgt tgaggaaata tcacgcaaag
    76621 gtaattcttg gatgaatatg ctggtttaga agatttattt agagcaatcg acaatagcca
    76681 ggtaaaaacg tgacacatgt caccaaattt aatgaagaga atttttttaa cgggggaggt
    76741 tcccccgtca gatcatttca caatggtttt gacaccgtca ggtgtgccaa tcagcgcaac
    76801 gtccgcgcca cggttagcaa acaagccaac agtcaccacg ccaggaatcg catttatggc
    76861 gttttccatc gctatcgggt caaggatttc catgccgtgg acgtcgagga tcacgttgcc
    76921 attatcggtc accacgccct gacggtattc cggacgaccg cccagtttca ccagctggcg
    76981 cgccactgca ctacgtgcca tcgggataac ttctactggc agcgggaatt tacccagaat
    77041 atcaacctgc ttggaagcgt ctgcaataca gataaatttt tctgcaaccg aagcaatgat
    77101 tttttcacgg gtcagcgccg cgccgccgcc tttgatcatt tgcatgtggc cgttgatttc
    77161 atctgcgcca tcaacgtaga tgccaaggct gtcgacttcg ttgagatcaa aaacgtgaat
    77221 gccgaggctt ttcagttttt cagtggaagc atctgaactg gaaacggccc cttcaatctg
    77281 gcctttcatt gtaccgagcg cgtcaataaa gtgtgcggcg gtggaacctg tacctacacc
    77341 aacaatggtg ccgggctgaa catactgaag tgccgcccat cctactgctt ttttcaattc
    77401 atcctgcgtc atgatcgttt cgcctgtggt atgaaatttc acacgcatta tatacaaaaa
    77461 aagcgattca gaccccgttg gcaagccgcg tggttaactc atccataaaa tatcgcgcaa
    77521 tggcgggcat cccctttcgc cccgcaaata aagcatacaa cggtctgggt atgccgctcc
    77581 acggtgcgaa cagaggcaat aacgccacgc cattcaacac aaccagggcg cgaactcgct
    77641 gagcgaaatg cattagcgca aatccctgat cttttaagct gcgcgtttaa ccacgaatac
    77701 aaatgttacc cgccgacggc ttcggtatat gcaacctgac acaaaattgt gtcatagtgc
    77761 aggaaaaagc atttaccagg agcagacaac agcaatgaaa cgcccggact acagaacatt
    77821 acaggcactg gatgcggtga tacgtgaacg aggatttgag cgcgcggcac aaaagctgtg
    77881 cattacacaa tcagccgtct cacagcgcat taagcaactg gaaaatatgt tcgggcagcc
    77941 gctgttggtg cgtaccgtac cgccgcgccc gacggaacaa gggcaaaaac tgctggcact
    78001 gctgcgccag gtggagttgc tggaagaaga gtggctgggc gatgaacaaa ccggttcgac
    78061 tccgctgctg ctttcactgg cggtcaacgc cgacagtctg gcgacgtggt tgcttcctgc
    78121 actggctcct gtgttggctg attcgcctat ccgcctcaac ttgcaggtag aagatgaaac
    78181 ccgcactcag gaacgtctgc gccgcggcga agtggtcggc gcggtgagta ttcaacatca
    78241 ggcgctgccg agttgtcttg tcgataaact tggtgcgctc gactatctgt tcgtcagctc
    78301 aaaacccttt gccgaaaaat atttccctaa cggcgtaacg cgttcggcat tactgaaagc
    78361 gccagtggtc gcgtttgacc atcttgacga tatgcaccag gcctttttgc agcaaaactt
    78421 cgatctgcct ccaggcagcg tgccctgcca tatcgttaat tcttcagaag cgttcgtaca
    78481 acttgctcgc cagggcacca cctgctgtat gatcccgcac ctgcaaatcg agaaagagct
    78541 ggccagcggt gaactgattg acttaacgcc tgggctattt caacgacgga tgctctactg
    78601 gcaccgcttt gctcctgaaa gccgcatgat gcgtaaagtc actgatgcgt tactcgatta
    78661 tggtcacaaa gtccttcgtc aggattaatc catcaaataa tgcctgatag cacatatcag
    78721 gcgttgtcct cacttctttt tgtattcctt gaatcacatc acaaaataga caaatctcag
    78781 gcggcaaaaa acgacgtctg aatgcatttt ttttgctggc gacaaaccca cgtaaaaagc
    78841 tcaccgtaag cgcaaatacc ctcattttga ttgcgtttta cggagcaaat aatgtctaac
    78901 gagcaggagt ggcaacagct tgccaacaag gaattgagcc gtcgggagaa aactgtcgac
    78961 tcgctggttc agcaaaccgc ggaagggatc gccatcaagc cgctgtatac cgaagccgat
    79021 ctcgataatc tggaggtgac aggtaccctt cctggtttgc cgccctacgt tcgtggcccg
    79081 cgtgccacta tgtataccgc ccaaccgtgg accatccgtc agtatgctgg tttttcaaca
    79141 gcaaaagagt ccaacgcttt ttatcgccgt aacctggccg ccgggcaaaa aggtctttcc
    79201 gttgcgtttg accttgccac ccaccgtggc tacgactccg ataacccacg cgtggcgggt
    79261 gacgtcggca aagcgggcgt cgctatcgac accgtggaag atatgaaagt cctgttcgac
    79321 cagatcccgc tggataaaat gtcggtttcg atgaccatga atggcgcagt gctaccagta
    79381 ctggcgtttt atatcgtcgc cgcagaagag caaggtgtta cacctgataa actgaccggc
    79441 accattcaaa acgatattct caaagagtac ctctgccgca acacctatat ttacccgcca
    79501 aaaccgtcaa tgcgcattat cgccgacatc atcgcctggt gttccggcaa catgccgcga
    79561 tttaatacca tcagtatcag cggttaccac atgggtgaag cgggtgccaa ctgcgtgcag
    79621 caggtagcat ttacgctcgc tgatgggatt gagtacatca aagcagcaat ctctgccgga
    79681 ctgaaaattg atgacttcgc tcctcgcctg tcgttcttct tcggcatcgg catggatctg
    79741 tttatgaacg tcgccatgtt gcgtgcggca cgttatttat ggagcgaaga ggtcagtgga
    79801 tttggcgcac aggacccgaa atcactggcg ctgcgtaccc actgccagac ctcaggctgg
    79861 agcctgactg aacaggatcc gtataacaac gttatccgca ccaccattga agcgctggct
    79921 gcgacgctgg gcggtactca gtcactgcat accaacgcct ttgacgaagc gcttggtttg
    79981 cctaccgatt tctcagcacg cattgcccgc aacacccaga tcatcatcca ggaagaatca
    80041 gaactctgcc gcaccgtcga tccactggcc ggatcctatt acattgagtc gctgaccgat
    80101 caaatcgtca aacaagccag agctattatc caatagatcg acgaagccgg tggcatggcg
    80161 aaagcgatcg aagcaggtct gccaaaacga atgatcgaag aggcctcagc gcgcgaacag
    80221 tcgctgatcg accagggcaa gcgtgtcatc gttggtgtca acaagtacaa actggatcac
    80281 gaagacgaaa ccgatgtact tgagatcgac aacgtgatgg tgcgtaacga gcaaattgct
    80341 tcgctggaac gcattcgcgc cccccgtgat gatgccgccg taaccgccgc gttgaacgcc
    80401 ctgactcacg ccgcacagca taacgaaaac ctgctggctg ccgctgttaa tgccgctcgc
    80461 gttcgcgcca ccctaggtga aatttccgat gcgctggaag tcgctttcga ccgttatctg
    80521 gtgccaagcc agtgtgttac cggcgtgatt gcgcaaagct atcatcagtc tgagaaatcg
    80581 gcctccgagt tcgatgccat tgttgcgcaa acggagcagt tccttgccga caatggtcgt
    80641 cgcccgcgca ttctgatcgc taagatgggc caggatggac acgatcgcgg cgcgaaagtg
    80701 atcgccagcg cctattccga tctcggtttc gacgtagatt taagcccgat gttctctaca
    80761 cctgaagaga tcgcccgcct ggccgtagaa aacgacgttc acgtagtggg cgcatcctca
    80821 ctggctgccg gtcataaaac gctgatcccg gaactggtcg aagcgctgaa aaaatgggga
    80881 cgcgaagata tctgcgtagt cgccggtggc gtcattccac cgcaggatta cgccttcctg
    80941 caagagcgcg gcgtggcggc gatttatggt ccaggtacac ctatgctcga gagtgtgcgc
    81001 gacgtactga atctgataag ccagcatcat gattaatgaa gccacgctgg cagaaagtat
    81061 tcgccgctta cgtcagggtg agcgtgccac actcgcccag gccatgacgc tggtggaaag
    81121 ccgtcacccg cgtcatcagg cgctaagtac gcaactgctt gatgccatta tgccgtactg
    81181 cggtaacgcc ctgcgactgg gcgttaccgg cacacccggc gcggggaaaa gtacctttct
    81241 tgaagccttt ggcatgttgt tgattcgtga gggattaaag gtcgcggtta ttgcggtcga
    81301 tcccagcagc ccggtcactg gcggtagcat tctcggggat aaaacccgca tgaatgacct
    81361 ggcgcgtgcc gaagcggcgt ttattcgccc ggtaccatcc tccggtcatc tgggcggtgc
    81421 cagtcagcga gcgcgggaat taatgctgtt atgcgaagcg gcgggttatg acgtagtgat
    81481 tgtcgaaacg gttggcgtcg ggcagtcgga aacggaagtc gcccgcatgg tggactgttt
    81541 tatctcgttg caaattgccg gtggcggcga tgatctgcag ggcattaaaa aagggctgat
    81601 ggaagtggct gatctgctcg ttatcaacaa agacgatggc gataaccata ccaatgtcgc
    81661 cattgcccgg catatgtacg agagtgccct gcatattctg cgacgtaaat acgacgaatg
    81721 gcagccacgg gttctgactt gtagcgcact ggaaaaacgt ggaatcgatg agatctggca
    81781 cgccatcatc gacttcaaaa ccgcgctaac tgccagtggt cgtttacaac aagtgcggca
    81841 acaacaatcg gtggaatggc tgcgtaagca gaccgaagaa gaagtactgc atcacctgtt
    81901 cgcgaatgaa gatttcgatc gctattaccg ccagacgctt ttagcggtca aaaacaatac
    81961 gctctcaccg cgcaccggcc tgcggcagct cagtgaattt atccagacgc aatattttga
    82021 ttaaaggaat ttttatgtct tatcagtatg ttaacgttgt cactatcaac aaagtggcgg
    82081 tcattgagtt taactatggc cgcaaactta atgccttaag taaagtcttt attgatgatc
    82141 ttatgcaggc attaagcgat ctcaaccggc cggaaattcg ctgtatcatt ttgcgcgcgc
    82201 cgagtggatc caaagtcttc tccgcaggcc acgatattca cgaactgccg tctggcggtc
    82261 gcgatccgct ctcctatgat gatccattgc gtcaaatcac ccgcatgatc caaaaattcc
    82321 cgaaaccgat catttcgatg gtggaaggta gtgtttgggg tggcgcattt gaaatgatca
    82381 tgagttccga tctgattatc gccgccagta cctcaacctt ctcaatgacg cctgtaaacc
    82441 tcggcgtccc gtataacctg gtcggcattc acaacctgac ccgcgacgcg ggcttccaca
    82501 ttgtcaaaga gctgattttt accgcttcgc caatcaccgc ccagcgcgcg ttggctgtcg
    82561 gcatcctcaa ccatgttgtg gaagtggaag aactggaaga tttcacctta caaatggcgc
    82621 accacatctc tgagaaagcg ccgttagcca ttgctgttat caaagaagag ctgcgtgtac
    82681 tgggcgaagc gcacaccatg aactccgatg aatttgaacg tattcagggg atgcgacgcg
    82741 cggtttatga cagcgaagat taccaggaag gaatgaacgc tttcctcgaa aaacgtaaac
    82801 ctaatttcgt tggtcattaa tccctgcgaa cgaaggagta aaaatggaaa ctcagtggac
    82861 aaggatgacc gccgatgaag cggcagaaat tatccagcat aacgacatgg tggcatttag
    82921 cggctttacc ccggcgggtt cgccgaaagc cctacccacc gcgattgccc gcagagctaa
    82981 cgaacagcat gaggccaaaa agccgtatca aattcgcctg ctgacggggg cgtcaatcag
    83041 cgccgccgct gacgatgtac tttctgacgc cgatgctgtt tcctggcgtg cgccatatca
    83101 aacatcgtcc ggtttacgta aaaagatcaa tcagggcgcg gtgagtttcg ttgacctgca
    83161 tttgagcgaa gtggcgcaaa tggtcaatta cggtttcttc ggcgacattg atgttgccgt
    83221 catcgaagca tcggcactgg caccggatgg tcgagtctgg ttaaccagcg ggatcggtaa
    83281 tgcgccgaca tggctgctgc gggcgaagaa agtgatcatt gaacttaatc actatcacga
    83341 tccgcgcgtt gcagaactgg cggatattgt gattcctggc gcgccaccgc ggcgcaatag
    83401 cgtgtcgatc ttccatgcaa tggatcgcgt cggtacccgc tatgtgcaaa tcgatccgaa
    83461 aaagattgtc gccgtcgtgg aaaccaactt gcccgacgcc ggtaatatgc tggataagca
    83521 aaatcccatg tgccagcaga ttgccgataa cgtggtcacg ttcttattgc aggaaatggc
    83581 gcatgggcgt attccgccgg aatttctgcc gctgcaaagt ggcgtgggca atatcaataa
    83641 tgcggtaatg gcgcgtctgg gggaaaaccc ggaaattcct ccgtttatga tgtattcgga
    83701 agtgctacag gaatcggtgg tgcatttact ggaaaccggc aaaatcagcg gggccagcgc
    83761 ctccagcctg acaatctcgg ccgattccct gcgcaagatt tacgacaata tggattactt
    83821 tgccagccgc attgtgttgc gtccgcagga gatttccaat aacccggaaa tcatccgtcg
    83881 tctgggcgtc atcgctctga acgtcggcct ggagtttgat atttacgggc atgccaactc
    83941 aacgcacgta gccggggtcg atctgatgaa cggcatcggc ggcagcggtg attttgaacg
    84001 caacgcgtat ctgtcgatct ttatggcccc gtcgattgct aaagaaggca agatctcaac
    84061 cgtcgtgcca atgtgcagcc atgttgatca cagcgaacac agcgtcaaag tgatcatcac
    84121 cgaacaaggg atcgccgatc tgcgcggtct ttccccgctt caacgcgccc gcactatcat
    84181 tgataattgt gcacatccta tgtatcggga ttatctgcat cgctatctgg aaaatgcgcc
    84241 tggcggacat attcaccacg atcttagcca cgtcttcgac ttacaccgta atttaattgc
    84301 aaccggctcg atgctgggtt aatgactcgt cccgtcacga tcttcagcaa tatgctgaag
    84361 catcgtggcg gtataacgat gatttctgat tgaataaaga tattcctgca tatacatcgg
    84421 cctgtgcggc gcatcaaaat atttcagcgt tgccggattc accagtcgcc aggcaaaatg
    84481 cgggatcacc gtcagaaaat gtccgcgctc cacggcgctg attttggcca taaaactgta
    84541 gggacgataa ataatggtgg gattgatccc acaaggtcgc atattcgcat caatcattgc
    84601 ttcgaagtta gcccggtttt gaaagcgcat ttgtagccag ggcagctcct gaagcagctc
    84661 ttgctcttga cgctcttcat aacggcgaga aacgagaaag cccaatcgca gcggtggtaa
    84721 ttcactgata gtcaggtttt ccagttcctg aactcgtgca gatacatgct gtggggagat
    84781 aataaaatct aactgacggt caaaaagatt atcgataacg ccgttttcgc tgaactcaac
    84841 tggctgggcg gtcacgcctt catacttatc tccgagacta atcaactgat cgaaaataat
    84901 tgtcggataa gtattatcta tgccaatcac aatttctcgt gaacgacgcc ctgagttatg
    84961 gatttcatta tcaatcgcag aaagtcgttg ataaaccggg aacagttttt gatacagttc
    85021 ctgaccggct ttattgaggc tgatattatt atctttgcgg gtaaatagcg tatagccaat
    85081 ttgttcttcc agcgcggcaa tgcttttacc aaatggcgaa gccgtcatat ggattttttc
    85141 tgccgcccgg gcaatattat tggtttgcgc taataaaatg aaattgcgca tctttttcga
    85201 aataaaaatg tccatccccc ctccccgcat ccttgtccgc ttaaccgttt cagtcagcct
    85261 atccttgatg aaaccgcgag caaagatagg tgattacgtc atggttttac agaaaattac
    85321 agaaaaagga ggcaatatcg ggtaaaggca ttagcccgac gaatacgtcg ggctacaagt
    85381 attattgtgc tgcaggtgtt ttagcgggtt gttgatccac aggttctaac tggaagacca
    85441 catcgacctg atcatcaaac tgaatagcgg cctgctcgta agtttcctgg gcagacaccg
    85501 gcgcggcatc ggctttcatc atccgcacca ttgggctggg ctgatagttg gaaacatggt
    85561 agcgcacgct atataccggc cccagtttac gatgaaagcc gttcgccagt tcctgcgcct
    85621 gatgaatcgc gttatcaatc gctgccttac gcgctttgtc tttataggca tccggctgcg
    85681 ccacgcccag cgacacagaa cgaatttcgt taagacccgc cttcagcgcg ccatccagca
    85741 aggaattcag tttgtctaac tgacggagcg tgacttccac cgttctcaca gcgcggtagc
    85801 ctttcaggat acttttacca tcctgatagt cgtaatctgg ctgggtgcgt aaattcgctg
    85861 agctgatatc ttttttcgcg atctgattga gttcaaggaa ggaaatgtat tgtgcgacgc
    85921 gctcatccgc ctgtttcttg gcagtagcgg catccttcgc ggccacgttg acttcaatcg
    85981 caagagtggc aatgtctggc accgcatcca cgcttgccgt accggaggtg acaatatgcg
    86041 gtccatccgg caattcgtta gcctgcgctg ccatcccgct aatacccatt aatgccgcca
    86101 gggcgataac tttgaacttc acgttagtct ctccgtgtta attgacttgc ccaaaattca
    86161 ggacctctgg aggcaagctt agcgcctctg ttttattttt ccatcagata gcgcctaact
    86221 gaacaaggct tgtgcatgag caataccgtc tctcgccagc tgcaaggcaa taaaccacat
    86281 aacacatccc actaccagat tgataatgcg ctgtgctttt gctgtgcgca gacgcggtgc
    86341 cagccaggct gcgagaatag ccagaccaaa gaaccacaag aaagaggcac taattgtccc
    86401 gagcgcaaac caacgttttg gttcgacgtc aagttgcccg ccgaggctgc ccagtacaac
    86461 aaaagtatcc aggtaaacat gcggattcag ccaggtcact gccagcatgg tggcgataat
    86521 tttccatctg ccttgcttaa ggacttcggc actggctaac tcaatattac tgctcattgc
    86581 tgttttaaaa gcgccaaaac cataccagag caagaaggct acgccgcccc aggtgaccag
    86641 cgccagcaac cacggcgact gcatcaataa cgcgctgcca ccaaaaatcc ccgcgcaaat
    86701 caggaccaaa tcgctgatag cacaaagtaa ggcaatcata atgtggtact gacgacgaat
    86761 gccctgattc atcacaaaag cattttgtgg accgagcggt aggatcatag ccgccccaag
    86821 agcaagacct tgaaagtaat aagaaaacac gttggatatt ccgaattaag tatctgatgc
    86881 agagactata tcgcgggatt gttattagcg gaaattgata atattaattg gcaatcagaa
    86941 taactaataa gagagaggcc agagagtatt atctggcctc agcgttgatt acgcagcttt
    87001 gtcttctttc acccgcttaa agttcacatc catttgcggg tacgggaagc tgataccggc
    87061 ggcatcaaat tcacgtttaa tacgctccag cacatcccag tacacgtttt gcagatcgcc
    87121 gctgttgctc cagacgcgga ccacgaaatt aatcgacgat gcaccaagtt cgttcaggcg
    87181 cacagtcatt tcgcgatctt tcaaaatgcg atcttcagac tggataatat tggtcaggat
    87241 ctgcttaacc tgatcgatat cggaatcata cgccacgcca ataataaatt cgttacggcg
    87301 aactggctcg cgggagaagt taataatatt tccggcaata attttaccgt tcggaataac
    87361 gataatttta ccgtctgcag tacgcatggt ggtggagaaa atctgcacac tcagcacagt
    87421 accggctacg ccgcccaggt caacatattc tccggcacgg aacgggcgga acatgacaag
    87481 taacacgcca gcggccaggt tagacagtga cccctgcaaa gccagaccaa cagctaagcc
    87541 tgcggcaccg agtacagcaa tgacggacgc ggtttgtaca cccacgcgtc ccagtgcagc
    87601 gattagcgta aaggcgataa taccgtaacg gactaatgca gagagaaaat cagcaacagt
    87661 ggcatcgatt ttacgggaga tcatcaggcg attcaccgcg ttggaaatca tccgcgcgat
    87721 aatcaaacca acgatgatga tcgcgagtgc cgccacgatg tttactgcat aacttagcag
    87781 cagcgcctgg ttagctacca gccagcttcc cgcgccgttt atgctatcga caacattcaa
    87841 atcttccatt caatattcct tttcaactga ctcaaaatgg agaaatacac tccgccgcct
    87901 tatgacgggc agtctgacaa gggtaaacaa taaagcataa ttttgccaaa tagatcacag
    87961 atttctcatc tgaatcacct tgcttttggc gaaaaaatcc ccgcagagcg gggctcagac
    88021 agatatcaat gtgcgctttg tcatgccgga tgcggcgtga acgccttatc cggcctacaa
    88081 aaacctgcaa attcaatata ttgcaggata cgtgtaggcc tgataagcgt agcgcatcag
    88141 gcaattttgc ttttggcgaa aaaaagaccc gcagagcggg ccttgagata agcagaaagg
    88201 aatatcttac agaacgtcga tcgcgttcag ttcctggaat gctttttcca gacgagcgat
    88261 catcgaagtc tgaccggcac gcagccatac gcgcggatcg tagtatttct tgttcggctg
    88321 atcttcgcct ttcgggttac ccagctgacc ctgcagataa gcttcgttcg ctttgtagta
    88381 gttcagaacg ccttcccagg ttgcccattg ggtatcggta tcgatgttca ttttcactac
    88441 gccgtagctt acggagtctt tgatttcctg agcagtagaa ccggaaccac cgtggaatac
    88501 gaagttcagg ctgttgtgcg gcaggttgtg tttcttagaa acatattcct gagaatcacg
    88561 caggatggtc ggagtcagaa ccacgttacc cggcttgtaa acaccgtgta cgttaccgaa
    88621 ggacgctgcg atggtgaaac gcgggctgat tttgctcagc tcggtgtatg cgtaatcaac
    88681 gtcttccggc tgggtgtaca gtgcagaagc gtccatgtgg ctgttgtcca cgccgtcttc
    88741 ttcaccaccg gtgcaaccca gttcgatttc cagagtcatg ccgattttgg acatacgctc
    88801 caggtattta gagcagattt cgatgttctc ttgcagagat tcttcagaca ggtcgatcat
    88861 gtgagaagag aacagcggct tgccggtagc agcgaagtgt ttttcacccg cgtccagcag
    88921 accgtcgatc cacggcagca gtttcttcgc gcagtggtcg gtgtgcagga taaccggaac
    88981 accataatgt tcagccatct ggtgaacgtg atgcgcacca gagatcgcgc ccaggatagc
    89041 agcaccctgc ggaacgtcag atttcacgcc tttaccagcg ataaaggaag caccaccgtt
    89101 ggagaactga acgataaccg gcgctttaac tttagcagcg gtttccagta cggcgttgat
    89161 ggagtcagta ccgacgcagt ttactgctgg cagtgcgaag ttgttttctt ttgctacctg
    89221 gaaaactttc tgtacgtcat caccagtgat tacgccaggt tttacgaaat caaaaatctt
    89281 agacatgtct cttgtcctgt atcgtcgggc cttatactcg tcatacttca agttgcatgt
    89341 gctgcgtctg cgttcgctca ccccagtcac ttacttatgt aagctcctgg ggattcactc
    89401 tcttgtcgcc ttcctgcaac tcgaattatt tagagtatga aaaaatgcgt gctctaaaag
    89461 cgcgctgaaa caagggcagg tttccctgcc ctgtgatttt ttacttctta gcgcgctctt
    89521 cgagcatcgc tactgcaggc agtactttac cttccacgaa ttcgaggaat gcgccgccgc
    89581 cagtggagat gtaggagatt ttgtcagcaa tgccgaacag gtcgattgct gccagagtgt
    89641 cgccgccgcc agcgatggag aacgcttcgc tgtctgcgat agcgttagcc acgatttcag
    89701 tacctttgcg gaagttaggg aattcgaaca cgccaaccgg accgttccac agaatggttt
    89761 tcgcattctt caggatttca gccagttcct gagcggaagc atcaccgata tccaggatct
    89821 gctcgtcagc tttcacatcg ttaacagatt tcagggtagc cggtgcagtt tcagagaact
    89881 cggttgctac gcgaacatca gacggaaccg ggatgttgca ggtggtcagc agacgtttag
    89941 cttcgtcaac caggtcagct tcgtacaggg atttacccac atcgtggcct tgtgccgcga
    90001 taaaggtgtt agcgatacca ccaccaacaa tcagctggtc agcgatttta gacagggagt
    90061 ccagaacggt cagtttggta gatactttag aaccaccaac gatagccacc atcgggcgag
    90121 caggttcttt cagtgcttta cccagcgcgt ccagttcagc tgccagcagc gggcctgcgc
    90181 acgcaacgtc agcgaattta ccgataccgt gagtagaagc ctgcgcgcgg tgagcagtac
    90241 cgaatgcgtc cattacgaac acgtcacaca gtgcagcgta ttttttggac agggtttcgt
    90301 cgtctttctt ctcgcctttg ttgaagcgaa cgttttccag aacaaccagt tcaccttcag
    90361 caacgtcaac gccgtcgagg taatctttaa ccagacgaac cgggttagac agtttgtctt
    90421 tcaggtagtt aacaaccggc agcagagaga attcttcgtt gtactcgcct tcggtaggac
    90481 gacccaggtg ggaagttacc atcacttttg cgccttgttt cagggccagt tcgatggtcg
    90541 gcagagaagc acggatacgc gcgtcgctgg ttactttccc gtcttttact ggtacgttca
    90601 gatccgcacg gataaataca cgtttcccag caagatccag atcggtcatc ttaattacag
    90661 acatggtgaa tcctctcgtt gattctaaaa gttttgcaga cgctgcttgc gtcttacctg
    90721 aaagcaacag tagccatagc taacgtcgtg tcgagcattc ggttagcaaa gccccattcg
    90781 ttatcgcacc agaccaacgt tttgatcagg tgtgcgccac tgacccgggt ttgggtgcca
    90841 tcgacaatgg cactgtgcgg atcgtggtta aaatctacag agaccaacgg caattccgta
    90901 tagtcaacta taccatgaaa tgcaccttgt gctgcttttt gcagcaacag gttgacttca
    90961 ttggctttta caggtttctt caccgtcacg cttaaatcga ttgccgtcac atttatggtt
    91021 ggcacacgta ccgcaatcgc ttcaaagcga tcgttaaatt gcggaaaaaa tcgtgtgata
    91081 ccggcggcca gtttagtatc gaccggaatg atcgactggc tggctgcccg ggtgcgacgc
    91141 aggtcaggat ggtatgcatc aataacctgt tgatcgtgca tggcggagtg aattgtggtc
    91201 actgtgccgg actcaatacc gtacgcatca tctaacaatt tgatgacggg aattatgcaa
    91261 ttcgtggtac aggaagcgtt agaaacgatg cggtgttccg cacgaagttg atcctgattg
    91321 acgccgtaaa caacggtcgc gtcgagatcg ttactgccag gatgtgaaaa gagcactttt
    91381 ttggccccgg cggcaatatg cgcttcgcca tgctcgcggg agccatatac gccggtgcag
    91441 tcgaggacta catcaacgcc aagttcacgc caggggagcg attgcagtga acgttcatgc
    91501 aatacgcgga tggcgtcatc accaacaaaa agttgatcgc gttcctgtcg tacttcccat
    91561 gcaaaacggc catggctggt gtcatatttc aacaaatgcg ccatgcccgc agcatccgcc
    91621 agttcgttga ttgccaccac ggtaatttcc gcccggcgtc ctgattcata caaagcacga
    91681 accacattac gcccgatgcg accgaagcca tttatcgcta cgcgtacggt catagatctc
    91741 ctgcaaggtt ttccctgagc aaatttgcca gacagagtaa tccagcaaat cgtccgggga
    91801 aaccttacct gtcgcaaact gcgactgatt ggttaattgt cgaacattta atcgactgaa
    91861 acgcttcagc taggataagc gaaacgtgga ataaaaggaa tgattgtcca gtcaaagaaa
    91921 gcaattatct gacttacgtc acgttttcag ccccgcccct gtggaattta ttccactttg
    91981 cagaatcacg aataagcgta gcaaaatctt ttcgttgtgc cgtcacaaac cctgacttat
    92041 atcagaatat gactgatgca gcgtcgatat tatccgcaat acgtccgagt ttcggagtac
    92101 tccaatgatg acattgccct acccatattc ttcctccgca cgcttttacg cactgcgcct
    92161 cctccccggg caggaagttt tttcccagct acacgctttt gcacagcagc atcaacttca
    92221 tgccgcctgg attgcaggct gtaccggtag cctgactgac gtcgccttgc gctatgccgg
    92281 gcaagaaggc accacgctcc tgaacggtac attcgaagtg atctcactaa acggcacgct
    92341 ggaacagagt ggtgagcatc ttcatctctg cgtttccgat ccacacggca cgatgcttgg
    92401 cggtcatatg atgcccggct gtaccgtgcg gaccacgctt gaactagtga tcggtagcct
    92461 tgaggaactg gcgtttagcc gccaaccttg cgcgctttct ggttacgacg agttgcatat
    92521 ttcccccgtt aaataactac tttcaaagag gttttcttat ggcgcgtagc cacttttcca
    92581 gtcaggcact ggtgttgatc gttatatcta tcgccatcaa tatgatcggc gggcaactgg
    92641 ccagcatggt caaattacct atatttctcg actccatcgg cacacttatt agcgcggtac
    92701 tgcttggtcc ggtgatcgga atgctgaccg gcttactgac caatttactc tggggattgc
    92761 tgaccgaccc gatcgccgcc gcatttgcac ccgtcgcgat ggtcatcggc ctggtcgccg
    92821 gttggctggc gcgagcgggt tggtttcgca ccctgccaaa agtcgttgtc agcggcgtga
    92881 ttattacgct tgcggtaacg gtcgtcgctg taccgctgcg tactgagctg ttcggcggtg
    92941 tcaccggtag cggagccgat ttgttcgttg cctggatgca ctcgatgggg caaaacctgg
    93001 tggagtcggt ggcgattacg gtgataggcg cgaacctggt cgataaaatt cttacagcgg
    93061 tgattgtctg gcttttactg cgccagctgc cgattcgcac cacgcgccat tttccggcaa
    93121 tggctgccgt gcgctaaatg catccattta cctcattaac actgtgggca ttggcggcct
    93181 gcaccacgct tatcctgccc gcgcagacca ttttgccgat ctatagcgcg gcgacctttt
    93241 tctgccttat tgcgctaaag gccacgcggc ggcgggcaaa atatgtcgtc tggctgatgt
    93301 tctcgctggg agccgggctg tggctggtac acggtggctg gctaccagaa tggctaagcg
    93361 gcacgccacg ctcgccagaa cggtggtcgc atgctattac gctctggttg cggattctgg
    93421 cgattgtttc aacctcacag ttgtggatgc agtacgtccc cgtgcaatgt tttatccgcg
    93481 ctctgtttgc ttctcgcctg ccgccaggcg tcgcttatct gtttgccggg ccgttattgg
    93541 tcgttgagca gttaaaacgg caattagcga ttattcatga agcgcagcgt gcccgcggcg
    93601 tgccgctgga tgagggctgg tatcagcgtt tacgtgcgat gcccgcgtta attattccgc
    93661 taacacacaa tgcgctaaac aatcttgctg tccgtggtgc ggcgctggat atgcgggcat
    93721 ttcggatcca aaaccgacga acaacgttgt gggcaccagc cgatagcacc ctacaacgcg
    93781 tagcgcgtta caccatgatc ttgctgatgc tgacagaatt cggagcgtgg atatggttac
    93841 gttagaacag ttccgttatt gcccgacaca ctcaacgcat cctccgttct gttatgactt
    93901 tcactatgtc aaaccgggga tggtcgccat ctttggtgac aacggcagtg gcaaaagtac
    93961 gctggcacag ctgatggcgg gctggtatcc ggactttctg ccaggagaga taacaggcac
    94021 ggggacgctc ctcggtacgc ccattgggca tctcccgctt aatgaacaat ccgccaccat
    94081 ccagctcgtc cagcaatcgc cctatttgca gctctccggc tgtaccttta gcgtggaaga
    94141 agaagttgcc tttgggccag aaaatctgtg tcttgctgaa gcagagatta tggcgcggat
    94201 tgatgccgcc ctgactctga ccgagtgcca gccgttgcgc catcgtcacc cggcaacact
    94261 ctccggtgga gagacacagc gcgtggtcat cgcctgcgcc atcgcgatgc agccaaagct
    94321 cttaattctt gatgaagcct ttagccgcct gactccgcag gccagcgaaa tgctgttgca
    94381 gcgcttacaa cactgggcgt ttgaacgtgg ttcgctgatt attctttttg aacgtcatcg
    94441 cacacctttc ctgaactact gccagcaagc gtggcaattg caaaacggag cgctccagcc
    94501 actatgttaa cgttaaataa gatatcttat cgctggcctg gcgcggcaac ggattgtctt
    94561 tgcgacattt ctctgcaact caaacaaggt gaatggttgg cgctgaccgg cgacaatggc
    94621 gcgggaaaat cgacgctgtt acgagtaatg gcaggtcttc ttacgcccac agccggtacg
    94681 gttatgctgc aacaacaggc gatgaaaaac ctgaaaaacc gccagcgcgc ggcaaaggtt
    94741 ggcgtactgt ttcaggaagc ggaaaaccaa ctttttcaca gcaccgtcgc tgacgagatc
    94801 gcatttggtt tgaaactgca aaaatgcccg gcggatgaaa tcactcaacg tacccatgct
    94861 gccctgcaat gttgtcagtt agcagacact gccaactcgc acccgttgga tctgcatagt
    94921 gcgcagcgac ggatggtcgc cgtcgcctgc ctggaagcac tctctccgcc actattgctg
    94981 ctggacgagc cgagtcggga ttttgacgaa aattggttga gcgtgtttga aagctggctg
    95041 gagaagtgcg gccagcgagg cacgagcgtc gtagcaatca gtcatgacgc cgcatttacc
    95101 cggcgtcatt tctctcgtgt ggtgcgactg gaagatggtc taatcaggaa cgttaatcca
    95161 ccagacgata ttcacccgta gcggtcatca ttaaggtcaa atttgcaggc agactctctt
    95221 ccagcccccg gcgaacattc ggcccgtcgg tacggtcata aaaagcttct gcgtctgcca
    95281 gcgatacccc tcccgccagc ttgcgcccta ccagtcgttc ccgcaacgca gtagctggcg
    95341 cgttgataaa gatcgagaaa tcacaaaatt gcgcaagctg gcaccacttc tcgtcgtcca
    95401 gcaacaacca gtttccttcg acgatgacga gtggtgcggt aacgtgcaac gcatcttcaa
    95461 caggatcatg cttttgtcga tcatactgcg gccacgtaca atccccttcc actacctggc
    95521 acagattttc cgccagtttc gcaacgtcaa atgtctctgg tgcgcctttg aaggggcgca
    95581 attgatgcgc atccagccag ctattgtaat ggtgaaaacc atccatcggg agcgtctgga
    95641 tagcgggtaa ttcagggtct tgttgcgcga gatattccca gaaggtagtt aaggtagatt
    95701 tacccgtccc cggcggagcg caaagaaaaa ccaccgtacg tcgctgcgga cttaccgtct
    95761 gtaaggccgc cagcatacgc agcaacggtt tatgcacgcg ttctatctct tcatcatggt
    95821 actgcgcttg cacgttcagc ccattcaccg ttaattcaat tttcacggcg ttaattcctt
    95881 taaggtggtc gttactgcca gcgtcagcgc cgttttgacc attccgctaa aacgcgcctc
    95941 tgaccagacg gcgaaatcgg cgaacttcat cgcttttaac gcgtcataga gcgtcgcatt
    96001 gcaggttatg ctattgacgt tgctgataaa atcccaggtc atgtcatcgg caaatccggg
    96061 cgtttcatcc tgttcgttca ggtaatgcca gtactgggaa aagtgcgtga tatcggcata
    96121 cagccattta tccattttcc ccagcgcata aatcaaacgc agcgcaacat caatatcgtc
    96181 aagcgggccg ctttgggcga gtaacggctt cactgcatat tcgacgatct cttcatcgtt
    96241 atcgacaaac agcgacggca gaaactggcg tatcccctgg agcaaaatag aatgcgcagt
    96301 tttcataaat gaaaataaat tgtcctgtgc atccagttgc tcaagcacat catcttctgt
    96361 cagcgtcgcc attggtcttc cgatagatca ccatcaatgc agggagtcta ttatattaca
    96421 cgtttggtcc gcgccgccga tcagtaatgt ctgcgtccgc accccattcg ccgtctgttg
    96481 gataccgttc accaactcgc cgcccgtcac gcccgtagcg ctaaagagga tatcgttccc
    96541 cctcaccagt tcatcgagcg agtacacacg gttaacgtca acgcccattg ccttacaacg
    96601 cttgcgctcc tgctctgcaa tctgccgatt ttccgtgtaa tcccctttag cctgacaaaa
    96661 gtcaatcagc tcggcctgca tatccccacc taatgcttta acggcacagg cagaaatcac
    96721 gccttctggc gcaccgccga tggtgtacat cacgtcgtaa ggattatcct gccagcaggt
    96781 cagcacgctg gcggcgacat cgccatccgg cagagcgaaa accttcacgc ccagctgtgt
    96841 tgcttcttca atcgcggcac tcaggcgcgg tttatccagc gtaaccatgc gcagcttgtc
    96901 cagcggttta cctaacgccc gcgccacgtt acgcaagttg tccgccagag gcaaagacag
    96961 atcaatcgcc ccggcagcca gtcgattaac aaccagcttt ttcatataca tatcgggcgc
    97021 atgtaacagg ctatctcgag gggcgaaagc catgaccgcc agcgcattgc tctgccccat
    97081 cgctaccatc cgcgtgcctt caatcgggtc aaccgcgata tcgacttctg gcccatcgcc
    97141 tttgcccacc tcttcgccaa tccacagcat cggcgcatgg tcaatttctc cttcgccgat
    97201 aaccactcgt ccacgaaaag cgacgtcgtt taatgcctgg cgcatcgcgg tgaccgccag
    97261 gccatcaatt ttatttttgt cgccacatcc ggtttgcggc caggcagcca acgctgcctg
    97321 ttccgtaacg cgaaataatg gccacgccag ggacatcatt gcgcggcctc cccgatatca
    97381 acgccaaatt gtgccagcag atatttctcg gcctgttcat tccagatgcc gtgcgtttcc
    97441 tcaaccagcc gcgccaactc tttaaacagc ggatcggttt tgcctttttc ggcaaaatcg
    97501 gcaatggcag tgagcggcat ggtcacgccg ttataaatga gttttttacc gccagggata
    97561 tccggcagat tgagcacggt atctggcacc gcatccagcc caccgatatg cgtgaccata
    97621 aaggacggct gcaactgccc cgtggcgctt aacgcaatcg cctctttcat gtcgtccgtt
    97681 gaaccgccgg atgtaccgac tacgtgcgtg ctgttgtaat ggacgttgta gaaattaaac
    97741 ggcactttga agtttttatc cgtcggcccg gcaaagaagt tcagacagcc atcttccgcc
    97801 agtaattcgt cagccatctc aacgacagca ggcaccgccg cataaacaaa aatgtcatcg
    97861 aacccgacat ctcctgtcag cgcccgcagc gtctggaccg gatcgctcat ccctttggta
    97921 ttcacataca ccagctcaat gcctttactg gccgccagat ccaccggcag cagcttctgc
    97981 acctgcgcca ggcgtttgtc gtcgatatcg accaccacca cccgtgacgg ttgtatgcca
    98041 ccgttaatgg cgtaatcgat agcgccaatg cccatcggac ccgcacacgc cagcagtgca
    98101 atattgccgc caggtttgac gcccatgcga tgctcataaa catattgcgt ggtgtgataa
    98161 ttggcatgat aagcaccaat aatgcagcac ataggctctg ccagcgacgc cgcagcaaaa
    98221 taagagccgt gatacggtaa tacgcagccc aaattaatgg cgatttcggg aataatcata
    98281 taggtggcgt taccgccaaa atattcgtag ctgtagcctg ctgaatatcc gcttggtaac
    98341 cccatcgccg gttgcaatac aaaacgctgg ccttttttat atttgccagt gagattttta
    98401 cccacttcga caataacccc ggcacattca tgcccggtaa tgaccggatg attttctaaa
    98461 tcgtcgggta cgcgtttatg ttcactaccg agtaacgccg ctttccaggt cgataaacag
    98521 acgctgtcag aaattacact cactaacagt tcattatcgg taatttctgg cagttcaaat
    98581 tcgcgcagac ggacatcccg cttgccataa atagcagcaa ctttggtttt catttttacc
    98641 tctgtaatca gtgtttatgt tcggcggtta attgattaaa taaagtgtcg agttttggat
    98701 cgccaatata gttattaatc aatatcagcg gtttatccgt aacacgtttc acgcgccctt
    98761 ccagactggc atgagtaacg acgatatcgg catccgcagg cacgttttct atcgcgtaat
    98821 gttttacttc tattgccagc cccgcttttt ccaggcgttt acggaaggtg gtcgcgccca
    98881 tcgcactgga gcccatcccc gcatcgcaaa caaaggcgat acgcttaacc ctgcttaacg
    98941 agaatgcacc ctcttgtttc atcgccttaa ccgcattggc tgactgagca aactcatctt
    99001 cgctctccgt ttccaccgtt ttttccatct tcagtatcag cgaagtaata gcaaaggaca
    99061 ccagggtacc taccgtaaca ccggcaattg tcgccaggaa cgagcctttc ggcgtcagtg
    99121 ccaggtaagc aaagatagac cccggacttg ggccagccac cagaccaccg tccagtaagt
    99181 taaacatcca ggtaccagac ataccgcccg caatcatggc aataatagtc agcggcttca
    99241 tcagcacata tgggaaatac agttcgtgga tcccaccgag gaagtgaata atcatcgccc
    99301 cgggcgcaga acgtttactc atccctttac caaacaaggt aaacgccagc agcagcccca
    99361 ggcccggacc tgggttagag gccaccataa agaagatgga tttaccgtta accgaagcct
    99421 gttgcattcc cagcggataa tagacgccct gatcgatcgc attattaagg aaaagcactt
    99481 tcgccggttc attgatgact gacagcagag gcagataacc cgcatgtacc agcgcctcga
    99541 tacactcttt gacgaaagta ttggcaatta acaccgccgg gccgatcact tcaaaaccca
    99601 gcagacaaag gagcatcccc gcgataccta atgagaagtt attgataacc atctcaaaac
    99661 cggcaggtat gcgtttttcc agtgccttat cgacatattt tatgaccaac ccaccgagcg
    99721 gccccatgat cattgagcca aggaacatcg ggatctctgc gccaacgatc acaccaatag
    99781 tacctattcc gcccatgacc gccccgcgtt taccgccgac cagatgacca cctgtagaac
    99841 caatcatcac gggcaataaa taggtaatca tcgggccgac aattttggcg aaatgttcgt
    99901 taggcagcca accggtggga ataaataacg cagtaataaa accccaggca ataaaagcac
    99961 caatattggg gatgaccatt gcagtcaaaa agcccccaaa agcctggacc tttgcacgag
   100021 cagacttgtt ttccataata ttatcctgta gaggagagag taattatccg cggctgataa
   100081 tgtccgctaa ttgtttttct gatgatgctg ttaagagttg ttcgagtatt tcttcttcgc
   100141 acagtaattc acttaacgcc tgaatagcgc caatgtgcga atcggcatcg gcagcagaga
   100201 gtccgatgag taatttaatt ggttcgtcat tccccggaaa ataaacacct tgttcaagta
   100261 atgtcaacga catcccggtt ttaagcgccc cacattccgg tcgcgcatga ggcattgcca
   100321 cgcctggtgc gagaatataa taagggccat tgttaatggt ggaatcttta attgcctgaa
   100381 tgtaattctc gctgatatag tttttatcca gcaatgatac catcgagaaa tcgatagctt
   100441 cctgccaatc ttttgctgaa tgtataactg agattgatga ttctggaaaa taatcactaa
   100501 gccgcataaa ttatccttat tttattttta gggtaccgtc cggcggtaaa aataaaatac
   100561 tcgtgcggtt taatgaaagc aattgagcag ggaaaccgat gttttgaaaa cagtgaatta
   100621 ttaatttctt atataacatt gagttataga tataacaaca aatgtcaata cgcatatcgt
   100681 gatgcatatt acataattga tatttattgc atataaatat ttgtgatcta caacacgctt
   100741 tatctattgc ttgtccgcaa acggacatat caaggtaata aaaaaggtcg ccgaagcgac
   100801 ctttttaccc gaaatgctaa ttacagcagt tcttttgctt tcgcaacaac gttatcaaca
   100861 gtgaagccga actcttcaaa cagcagctct gccggagcag attcaccgaa ggtggtcata
   100921 ccgacgatag caccgttcag gccaacatac ttgtaccagt agtcagcaat acccgcttct
   100981 acagcaacgc gtgcagtaac cgctttcggc agtacggatt cacggtaagc agcatcctgc
   101041 ttgtcaaatg cgtcggtaga cggcatggac accacgcgcg ctttcacgcc ttcggcagtc
   101101 agtttttcgt aggcagcaac agccagttca acttctgaac cggtagcgat gaaaatcagt
   101161 tccggctgac cggcgcagtc tttcagcaca taaccaccgc gcgcgatgtt tgccagttgc
   101221 tcttcagttc gttcctgctg cgccaggttc tgacgggaga ggatcagtgc ggtcgggccg
   101281 tcctgacgct caacaccgta tttccacgcg accgcggatt caacctggtc acacggacgc
   101341 catgtagaca tgttcggggt tacgcgcaga gaagcgacct gctcaaccgg ctggtgagtc
   101401 gggccgtctt cgcccagacc gatggagtcg tgggtgtaaa ccatcacctg acgctgtttc
   101461 atcagcgcag ccatacgtac ggcgttacgt gcgtattcca cgaacatcag gaaggtggag
   101521 gtgtacggca ggaaaccacc gtgcagggag ataccgttag caatcgcggt cataccgaac
   101581 tcgcgaacac cgtagtggat gtagttaccc gcagcatctt cgttgattgc tttagaacca
   101641 gaccacaggg tcaggttaga cggtgccagg tcagcagaac cgccgaggaa ttccggcaac
   101701 agcggaccga aagcttcgat agcattctga gacgctttac ggctggcgat tttcgccggg
   101761 ttagcctgca gtttagcaat gaactctttt gctttcgcgt cgaagtcaga cggcatttcg
   101821 cctttcatac ggcgggtaaa ttcagcggct tcctgcggat aagctttcgc gtaagcagcg
   101881 aatttctcgt tccatgcgga ttctttcgcc tggcctgctt ctttcgcatc ccactgagca
   101941 tagatttcag acgggatttc gaacggtgca tatttccagc ccagctgttc gcgggtcagg
   102001 gcaatttcag cgtcgcccag cggcgcaccg tgggagtcgt gagtaccggc tttgttcggg
   102061 gaaccgaaac cgatgatggt tttgcacatc agcagggacg gtttgtcagt cactgcgcgc
   102121 gcttcttcta ctgcgcgttt gatggatgcc gcgtcatgac cgtcgatgtc gcgaataacg
   102181 tgccagccgt aagcttcgaa acgcattgcg gtgtcgtcgg tgaaccagcc ttcaacgtga
   102241 ccatcaatag agataccgtt gtcatcgtag aacgcaatca gtttacccag cttcagcgta
   102301 cccgccagag agcaaacttc gtgggagatg ccttccatca tgcagccgtc gcccatgaag
   102361 gcataggtgt agtggtcgac aatgtcgtgg cccggacggt taaactgcgc cgccagcgtt
   102421 ttttctgcaa tcgccatacc gactgcgttg gcaataccct gacccagcgg accggtggtg
   102481 gtttccacac cagcggtgta acccacttcc gggtgacccg gagttttaga gtgcagctga
   102541 cggaagtttt tcagttcttc catcggcaga tcgtaaccgg tgaggtgcag caggctgtag
   102601 atcagcatgg agccgtggcc gttggacagc acgaagcggt cacggtcagc ccaggacgga
   102661 ttctgcgggt tgtgtttcag gaaatcacgc cacaggactt cggcaatgtc agccataccc
   102721 ataggggcac ccgggtgacc ggatttggct ttctgtactg cgtccatgct cagcgcacga
   102781 atagcattgg caagctcttt acgtgaggac attttgactc cagatcggat gatgaagggc
   102841 acgcccttaa cgacttgacg acagcgcgtt ttgggctacg ccggaaaatt tgccaacaat
   102901 ttaccgcaag ccgcgcgtca tgtacatgga acatcctttt gccgcttcag aaatctctgg
   102961 atcatgctcg catgttgcgc aatctactcg cccgtccgct gcgcttttcc ttatactgag
   103021 actgagcgtc gattcacctg caaacggcgc atttttagaa taatcctgac cttgtgcgga
   103081 agagaaaaca tgaaaattcg cgccttattg gtagcaatga gcgtggcaac ggtactgact
   103141 ggttgccaga atatggactc caacggactg ctctcatcag gagcggaagc ttttcaggct
   103201 tacagtttga gtgatgcgca ggtgaaaacc ctgagcgatc aggcatgtca ggagatggac
   103261 agcaaggcga cgattgcgcc agccaatagc gaatacgcta aacgtctgac aactattgcc
   103321 aatgcgctag gcaacaatat caacggtcag ccggtgaatt acaaagtgta tatggcgaag
   103381 gatgtgaacg cctttgcaat ggctaacggc tgtatccgcg tctatagcgg gctgatggat
   103441 atgatgacgg ataacgaagt cgaagcggtg atcggtcacg agatggggca cgtggcgtta
   103501 ggccatgtga agaaagggat gcaggtggcg cttggtacaa atgccgtgcg agtagctgcg
   103561 gcctctgcgg gcgggattgt cggcagttta tctcaatcac aacttggtga tctgggcgag
   103621 aaattagtca attcgcaatt ctcccagcgc caggaagcag aagccgatga ttattcttac
   103681 gatcttctgc gccaacgcgg catcagcccg gcaggtcttg ccaccagctt tgaaaaactg
   103741 gcaaaactgg aagaaggtcg ccaaagctca atgtttgacg accatcctgc atccgccgaa
   103801 cgcgcccagc atattcgcga tcgtatgagc gcggatgggg ttaagtaagt cagacatacc
   103861 gatctacaac ataattgtag gtcggtaacg cctgaatccc cgatcagagt agtgatttca
   103921 ctttatttag caaaacggct acatcatccg gtcgggtata tcccgtatca gggtctataa
   103981 tcgagctgta aacatgtggc ataatgcgcg gcacccccgc ctccagacaa gttttcagaa
   104041 tcacactgaa gttatccaaa tcaatcccgc cagtcggctc aatcagcgtc ataccattgc
   104101 gcgccgccgt ggtcgccagc gcgtatagct ccggaagcga cctctcgccg cccatcggaa
   104161 agaacttcgc cgcgtgtgcg cccatatcta gcatcattcg caccgccgta tcgcaggaga
   104221 cgcgggcagg cgtaccctgg ctgctgctaa cgccggtgga aatcagcact tcgcccggcg
   104281 tccccgtcgg gctaaccagc gcgttgacat gggtctgctc accgcccgta gccgcaagcg
   104341 cgcctgccgc gaaaccacaa ccggtaaagg tctggttaac gtgtgctgga tgaatctgcg
   104401 ccgcaatcat cgcagctttg taatactgcg ccgggtcacc cgcccccaga cctactgaaa
   104461 tggacggtat ttccgccatc caacgtttaa cttcctggat cccctcatcc accgaggaga
   104521 actgcgcgga aagcacgcca atcactgcgt ggccttcggc ggcgtcaaag atttcacggg
   104581 cattggcgat atctttcgcc agcacattaa ttgccacgcg attacgatag aaattaatct
   104641 tttgcatgat ctgcaatctc cttcagtcgc gccacgataa gtgccatttc cccttccgcc
   104701 accgtacgcg gatcaaggct aaaaacgccc tggtggaggt tataccgccg tgcataaatc
   104761 gcgatatcgc caccacgcag ttgcgcttcg acgacgcgag cgtcaactcc caattcctgc
   104821 gcatttactc gaatacgaat acgccagatt gcacgcccgg cttcatcctg ctcaatatca
   104881 gccgttaacc cgtgaatcgc agaaatcgct tcaaccactg gctggagctg ttccgcggtg
   104941 atgactgcct gcccctggtg ataattctcc agcgcgtaaa ccagcccaac catgttctcc
   105001 ttgccgattt tcatcgccct ggcaatgccg tgatgctgcg ctttgcaggc tgcaatccag
   105061 atttttttac cagtgataaa cccggaggtc ggcgcgttaa aggcttttgc gccgctgtag
   105121 accaccatat ccgctccgct caccacccag gcacgtaaat cctcttcggc ggcggcatcg
   105181 acaatcagcg gcaggttgta catctgcgcg acatgtgcga agtcctcgat gttcaacatt
   105241 cccttctgca cgcagtgatg cgacttcaca tacagcagcg cggcggtcgc gtcggtaatg
   105301 gcactctcca gctgccagag agccgccagg ttacttgaac ccacctccac aacgcgcccg
   105361 ccacctaagc ggatcgcgct gaggatcgat gcaccgtaat cgacgttatg gccgcgcagc
   105421 atcaccactt cattggccat gccggagcta tccggcatga gcgccacccg ggccttgtcg
   105481 ccgcgtgtaa tggcggcggc gacagcgatg gcgatccctg ctgaggcaca ggaggtaacg
   105541 tagctatcct ccgccccggt aaagcgagag accagctcgc cggttttgtc caccagctgg
   105601 tcaatctcca cgaaagagga tgccgcgcgc gcggtggcct gcatcacctc cggcgcgacg
   105661 ctggagacgc cgagaatcgt catcttgccg caggcgttaa tcacctgttt cagtcccagt
   105721 tgttgataga tattctgcgt catggcttac agcactccca gcagcgagca gaccacgctc
   105781 aacgccacaa tcgacagcag aatcgtggtg taacgcggac cctttttcac caaatagaag
   105841 tagatggcga acactgccgc cagcggcagc aggccgggcg cgatggagtc cagaatctgc
   105901 tgtaccacca cttcagagcc tttcagtgcg ctaattttta aaggtgaggt tatcttgaca
   105961 aagctcgcgg atagcgcgcc catcatgata agccccagta cgttagcgcc gtagataagc
   106021 tccttgatgc gccctccctg cagcagactg ataatcgaat cgcgccccag cgtatagccc
   106081 ttgtgcacca tgccgtagct gattgccagc gtaatcgccg ggtagagaat cagcggcaaa
   106141 ataccgccca tcgcgctgcc attggcggcg aacgggataa aaatagcaat caacagcggc
   106201 atcaccgccg cccagatgat ggagtcgccc atcccggcca gcggccccat caggccggtt
   106261 ttaatcccgg tgatagaggc atcgttaatc gcctcgccgc gggtcttttg ctcttccatg
   106321 gcgatggaga tcccctggat caccgcgcca aacgtctgct ctgagttgaa gaagttcaga
   106381 tggcgtttta acgcctccac ctgctcctcc ttttgcggat agagcttttt gataatcggc
   106441 gtcatcgagg cgcagaaaat caggctctgt aaacgctcat aggagctgga aacttccgcg
   106501 cccagccagt agataaacca cgctttggtg atatccgctt tggtcagcgt cccggactcc
   106561 cgcgcgcgct caaccagttc atgctgcatt acgtcgtcgc tcattatgcc gctccttcat
   106621 ttttcgccag gcctttaatc aggaaggcca cgcaggtgcc gaaaatcgcc atcgccatga
   106681 tgtccacctt gaggtacagc accgcgaaga atccgccgat aaaccacggc agcaggctct
   106741 ttttaccaat caccataatg gtgatggcga agcccagcgc cgggagaatc ccgcccatga
   106801 tttcaaacga gtgggtcagc cagtgcggca tcagcttgag gaaactttcc actacgttct
   106861 ggccgaagta gttggcggcg aacaccactg ggaaacgtag taccagcccc agcagcgccg
   106921 ggtagagaaa ggcgcagcgc ataatggctg acatattggc cgtttccgcg tgtttatcgg
   106981 ccatatgcac ccaagccgca tttagcgtgc gacgcaactg gtcgaggaat acgccgataa
   107041 cgccaaatgg aatcgccagt gcaatcgcca ggttcgggtc catattggcc ttaacggcga
   107101 taggaatggc gatacaggcc gccagcgccg ggtctgacgg cacattgccg cctggcgttg
   107161 aggtcacacc cagatagacc aactgcatcc ccgcgccgat aatcatcgcc gcaggcatat
   107221 tgccgagcag caggccgaca aagaccgcca ccaccactgg ctgtaacagc atggcggaga
   107281 aggtatagcc aaggcgcaac cgggcaaacc agtaatacag ccccatcagg cttgcaaaaa
   107341 gtaaggtatc cataatttgt tatcccatgt tcagagggtc agaatttttt caggatatcg
   107401 tccagcgact gcggtttatc ttccgggatg gtctggaaaa cagccttaat gccgcgactt
   107461 ttcaaatcgt taagaatgcc aacgtctttt tcatccagcg ttatgttctg gaacaccgct
   107521 ttacggtttg gcccgccgcc taaaccgcca acctgaatat cagccacgtc aaagccctgc
   107581 tgcaccgctt cctgaatcgc cgccagcgac gggaacagca ccagcacgtt gccgtcgccc
   107641 agctggttct ctttccacgc ggccgcgaag ctctggttgc taaagcagtc cactttgata
   107701 ttcggcggcg cggccatcag gtagatattt ttcataaacg gatcggcgtc cagctcgtcg
   107761 ctgaccaccg caatacggtt ggcctgagac tgccctaccc acttcgtcac cacctgtccg
   107821 tggataagac ggctgtcaat gcgacataac acgatgtttg ccatacgatt ttccttactg
   107881 ttgtaattga cgaatatggg agacgacgtc caggcaactg ttgcgcccag cttccaccag
   107941 atcggccacg caggcggtca gtgggccatg ttcgcgcttg tccagcgcct ccagcagcag
   108001 cgaggcatta agcccggaga tcaccgccac cggataatcg gcgctcagcc gcgccgccac
   108061 gttagaggtg gtgccgccga taaaatcggt cacgataagc gaaccttccg gcatggtttt
   108121 caccacggct tcgacgcgct ggtaaaactc gcccagcgta tcgacgggca tcagcgcgat
   108181 ttccgtcacg ccttcgattt cgcccatcac catgcgcaga ctgttgcaga gctgcagccc
   108241 ccagccgccg tgagttagca gcagaatttc aggtaacgga tgaataggta cagttgtggt
   108301 caaaatagcc tcctttgctc cagtgtttac atttcatcag gagcaaagga cgtgccagaa
   108361 tttgtgtcac gcacagagga aatgagattg gatggggcgt taagccccgt aaaccagggg
   108421 atcagctgta gagcagttcg tagatataaa aatattctgc gtccgataaa cggatagcgt
   108481 aagcctcttc aatcggcaaa aaggaggatt tgatgacgct aaaggcacga gcgtccagat
   108541 caagccggtt ttctagcgcc atctgtaacg gtttgcggtt aatcacgata cgctcgacca
   108601 tgcagcagca gtggatcaga aaacgtagtg tcacctggcg gctcggtttc agggagagcg
   108661 tgttggttaa gtagttcaga acatcctcga tctctttcag aattcgctgc gggttgagca
   108721 ccgaaatatg gttgatgatg ctctccatgg tcagcgcgct gataaagcgc atggcgctgc
   108781 gctccatctc cagtcggcgc tcgctgcttg agagatccgg cgtcagcagg ctgagcacca
   108841 gctccggccc ttgctcggaa aacagttcct ccagcgagat aaacggtata tccggcagtc
   108901 ccggctggaa ggtgccgacg atccctgcca gccgttcact ggcattaagc gcctgctgaa
   108961 tacgctccag gctgcgcacc tcgttatagt cgagaatcac catacgcgtg tcctgcgaca
   109021 tcagctcgcc aaagctctcc tccagcacct ttttgatctt ttccgccgtc cccattccgg
   109081 ttatacatga gataaccagc actttgccac cgctctcttg ctgcggcgtg cagagccgac
   109141 aagggatatt tttactctgc ataagcacag ccaattgcga tagatcgcca gtttcgtagc
   109201 ttaaatccag cccaacctcc agcaggctgg tcagagtgat atttggcaac aacagaacgt
   109261 caatctggaa cagcttgctg atggtgctgc caaaatggac cagcgatccg atatccacca
   109321 tcagtatcag ccgctgccag caccggtcct gaatcatccg ggtcagggtt tccagggtgt
   109381 catgcaccga ctgctcaaat ggcatatcaa ttgcgtagaa cagatcgcgc tccagcaccc
   109441 ggttcacata ttgcgcctgg ctcgttgcgg tggtcgcacc gtgagcgatt agaatcacgc
   109501 cgcaatccgg gctggtgtcg atgcgctggc ggtagtggcg acactctttc aggaacaggc
   109561 acagccagac cacctccgtc gccgggcact gaatatgcaa caactcattg attttgcggc
   109621 acagaagcgt ggcattgtca tactcatctt tacagcgatc cagaatcagg ctggatgaat
   109681 agagttgagg tataagccct cgccgcacat agccaatcag cgcaagaaag tgtttacgta
   109741 acgggttcgc caggttttct ggcaatgtaa atcctaatac ctgctccacg taaccgatca
   109801 gcagcgtcac acgctcctca atttgatcgc catagcgcgg tggatgcgcc gcactatggc
   109861 ggctataaag accatattca aaaatcgaac tgagtttgtt tttcaggata gccagcgttt
   109921 ctgcgggcgg cacgttactg ttgcgcagat tgacatattc acgagtcagg aaactgtaaa
   109981 acaggtcgct ctcttctatt tccgcgctgg tcgccaacga attttttaac gcgggcaacg
   110041 tacgcgcatc cacgttcaga cgatctttac cgccaaacaa cgcatcgacc agcagacgct
   110101 gctctggcgt ggtattaaac gacatttccg ccagtcgttt atctaactct agcgtatcat
   110161 tacgctcggt cattcccgac gcccacgcct gcgcgcacaa gaactgaata tcacttttaa
   110221 gctggccgat attgccttcc aggggtttac ttaacaacca atgcaagagc gttttatcaa
   110281 ggcttatggt acgttcaatt ttacgacttt cacgctgaaa gaagcttaca atcaattcga
   110341 gctgctcctc gaccgaacgc tggcgaatac ccggtaagtc aatacatacc tggatacgcc
   110401 gctggaaggt acgcaatagt gccgagttga ccgactctgt agtggcgcaa ataaggcgca
   110461 ccgaaattga atatgttggc ccacttgaac caagtgggcg gtattcacct ttatcgagaa
   110521 tagaaaagag cttctcttgt ccttcgtacg ggaggcgatg cacttcatcc agaagtaaat
   110581 aacctccgtc agcttgctcc accaatcccg ttttattctc acttgccccg gtaaatgccc
   110641 ccttatggtg accaaacaga tgtgaggaaa gcaactccgg attatgagca tattcggcac
   110701 agttaaagta aaccaacgga ggtggcgaac ctatggtgtg cttgcaagcg aagcaatgca
   110761 ttaactcagc aaagaatgtt ttgccaacac cagatgcacc ggttaacagc acatgtagtc
   110821 cgtggggata aagcaccgct gcgcggcctt tttctaccgc atcacggaga ctacggtcgt
   110881 atccgataag tccggaaaac ggatcatcat ctgtatattt ttctttcttt ggcaataaac
   110941 tagccactga atgaactatg cattcgcaat catccagttt tcgattaatg agcaattcta
   111001 gtgcgtgacg atgtagaaaa aaaacaggac gtccctgact ttttaccacc aggccttcat
   111061 tccataattg attaagatct ttactaaccg agttgcgtgc cagcccaagg ttaaaaccaa
   111121 tcgcctcagc agtaaatgct ttctccttcg ctaaatcaga cagttccagc ccatacgtca
   111181 gccgctccag ctccttgtga ataacatcaa tccgtctcat ttctttaccg cttaaaaaac
   111241 aatagttgtt cagaacatac actatttgta aacgtaagaa tgagaactat cgggaagaat
   111301 taatgttcct tgtggcaatc tggtttctga atattcgtgt tttaaatatt acttatggtc
   111361 tgaaacaacc aggcaggtga acagagctaa gaaaaagtag cgtagaaata taaaaacata
   111421 tatcgcactg atgaatgttt cccggcatga ctatgcacac cgggaaatat ttagtaaatt
   111481 actcgccctt tttcgccgcc tgaatataca gcatctccag cgccagcgtc gccgcagcca
   111541 gagcggtgat ttccgactga tcatatgccg gagccacttc cactacgtcc atcccaacga
   111601 tgttgagatc tttcaggccg cgtaccagtt taatagcgcg atcggaggtc aggccgccaa
   111661 tcactggcgt accggtgcct ggtgcaaaag caggatccag gcagtcgata tcaaaagtca
   111721 ggtaaaccgg catatcaccc acaatctgtt tcacttgggc gataacgtca tccacgctgc
   111781 gatcgttcac ctggcaggcg tccagcacgg taaagccgtt gtctttatca aactcggtac
   111841 gaataccaat ctgcacggaa tgattcgggt cgatcagacc ttctttcggc gcggtataga
   111901 acatagtgcc gtggtcaaat tcacaaccgt tcgcataggt atcggtgtgg gcgtcaaagt
   111961 gtaccagcgc cattttgccg aaatgcttcg catgagcacg cagcagcggc agcgtaacaa
   112021 agtggtcacc accgaaagag agcatacgct taccggcagc cagcagcttc tcggcgtgcg
   112081 cctgcagctt ctcgctcatc tcacgggcat cgccgaaagc atataccaga tcaccgcagt
   112141 ccacgacgtt cagacgctca cgcatgtcga aattccacgg gaagcggttg tgttcccagg
   112201 ccagattcgt cgaaacctga cggatagctg ccggaccgtg gcgaccaccc gcacgaccag
   112261 aagtggccat atcgaacggc acgccagtaa tcacccagtc agcatcgctg tcatatggct
   112321 ggaagttcat cggcaggcgt aaaaaaccaa aggcgttgga aaccagtgag ttatcgtatt
   112381 gatgacctaa ggtgctcatg gatggactcc tattacaaag tcgatataaa aaaacccttc
   112441 cgcgtcgtta ggcccgacga ggaagggttg gatttgcgaa acacaagttt gtgctaatta
   112501 tcgccttaaa tgggcgaggc atcaagcgag gttgctattt ccgacacggt ggcggcgcat
   112561 agccattgtt atctgccgga aaggcttgtc cagagcacgg cggcagcgcg ttttgcgccg
   112621 ggccttgtaa ttcaaaggcg gcaaagccga ttaccccggt attacgcaca tcgccttgcg
   112681 ccgaattggc tgcgtaggaa tcagcaacgt tggcgaactg aaaagccgct tcagtatgtt
   112741 tatctttgcg gaatccttta atcgctaacg aatctccggc attcacaata tagccgttgt
   112801 tattcagcga accttgcttg ccgttcagca catccaggcc atcaaccgta gcaacgatct
   112861 cgtagttggt gttacgactg taattacgaa catataattg ataattcatt ccgactctgg
   112921 cgtggacaat atagcgtggt tcattattgg atgaatcata aacacgcgta ataggtagag
   112981 aattaaaatt accatcgcga atagcatatt ccagttcgtt aatacgaatg ctataaacgc
   113041 ggtcgtagcc ggaaggaaca ttagtcgaat aactaataac gatcatctcc gccggatcgc
   113101 gccaggctcg ctcagcagat acgccctgaa ccgttgagtg cacatcccca ccccaggcgg
   113161 tgccgacatt acccgatgca ctgctattat actgcgcatt actggaacaa ccggccagca
   113221 gcacgattaa tgccaccaac gcgctacgta ctttccattt tttcataacc gttccctctg
   113281 catttaatta atgcagcgat tgtagggaga ttatttaata agaaaagcag ctaaattatt
   113341 tttaagaata tataaaaaga aagcggaccg atttacgtaa ataaatcgac ccgccagcag
   113401 aataatctta attattcatc ttcaagatag gtataaccgt acaaacctgc ctcgaactct
   113461 tcaaggaact gttgttgcag ttcagcatca agatcggttt tcttcacttg atcacggaac
   113521 tgagttaaca gcgttttcgg atcgagctgt acatattgca gcatgtccgc cacggtatcg
   113581 ccttcgtcag acagttccac ttcgacacta ccgtcaggga acacgaacac gtcaaccgct
   113641 tcggtatcac cgaacaggtt gtgcatgttg ccgaggatct cctgatatgc gccgaccata
   113701 aagaaaccga gcatcggcgg attctctgga tcgtactccg gcataggcat tgtcgtggca
   113761 ataccgtcac catcaatata gtggtcgata gcaccgtcag agtcacaggt aatatccagc
   113821 agcacagcgc ggcgttccgg cacttgatcc agcccttcca gcggcagaac cgggaacaac
   113881 tggtcgatcc cccacgcatc cggcatcgac tggaacagcg agaagttgac gtacattttg
   113941 tccgccatac gttcctgcag ctcgtcgata atcggacgat gagcacggtt ttgcggatcc
   114001 agctgctttt gcacttcatg gcacatgctc aaataaagct gctcagccca tgcgcgttct
   114061 tgcaggctaa aggtgccgga agagtagccg atatgaatgt cgtgcagatc catctgactg
   114121 tcgtgtaacc attcacgcag agaacggcga gttcccggtt cgtgcatctc ctgccaggtt
   114181 tcccacatgc tttgcagcgc acgcggcgca tcttctgcag gcgcggtcgg caccgtgtat
   114241 tcgttacgtt ccacgccgat gatattagac accagcacgg tgtgatgcgc agtcactgca
   114301 cgacccgatt cagtgattac cgtcggatgc ggcagaccat tttcttcaca cgcatcgcca
   114361 atcgcccaga tgatgttgtt ggcgtattca ttgaggccat agtttacgga gcagtcagac
   114421 tgcgaacggg tgccttcata gtccacgccc agaccgccgc cgacgtcgaa gcactgaata
   114481 ttgacgccca gcttgtgcag ttccacatag aaacgcgccg attcacgaac gcctgtggcg
   114541 atatcgcgaa tattcgccat ctgcgaaccg aggtggaagt gcagtagttg caggctgtcg
   114601 agacgcccgg cttcacgcag ggtttcaacc agttgcagta cctgagtcgc agccaggccg
   114661 aacttcgatt tttccccgcc ggaggactgc catttacccg agccctgcga agccagacgt
   114721 gcacgcacgc ccagacgagg aacgacattc agacgttctg cttcatccag cacaatggcg
   114781 atttctgaca tcttctcaat gaccagatag accttgtgcc ccatcttctc gccaattaat
   114841 gccaggcgga tatattcgcg gtctttataa ccgttgcaga cgatgacgct acgggtcatg
   114901 ccagcatgcg ccagcaccgc catcaactct gctttcgagc ctgcttccag ccccaacggt
   114961 tcgccagaat gaatcaggga ctcaatcacg cggcggtgct ggttaacttt gatcggataa
   115021 acaaggaagt aatcgccgtt atagccgtag gattcccgcg cacgtttgaa cgcggcgtta
   115081 atggaacgca aacggtgctg caggatctgt gggaaacaga acagtgcagg cagacgctga
   115141 ccctgtgctt cacgagtttt cactaactgc gcgagatcga cgcgagcttc cgggacgtcc
   115201 gggtccgggc acacgctgat gtggcccagc tcgttaacgt catagtagtt attgccccac
   115261 caggcaatat tgtaagtacg cagcatcttg ctggcttcct gggagctcat tgcaacctcc
   115321 tgcatggagc gtagtacacc gtgttcgccc gctgacgaag gcaaacccat agacatgtcg
   115381 tcagacatag cgaacctcaa attattttat taagtgtaaa acagttaacg actatcgcag
   115441 cctggaactg cgataacaac ccataagcac atggattttc cagcagtgaa tgctgacgct
   115501 ccaactgcgc aaccggtttc tttttcataa cattattaag cacataaccg aacgtaagtg
   115561 tgacagttcg gcgaaaccac gagaaaactc ttgtttttac aagagcgccc ttgttcagtc
   115621 ctcagtaact gtaaccagct cttgaatcct gagaagcgcc gagatgggta taacatcggc
   115681 aggtatgcaa agcagagatg cagagtgcgg ggaacgaatc ttcaccagaa cggtgagaca
   115741 ggttaagcag cagacaacgg ttcattattt cgtatcacct ccacggccgc ctgttaagac
   115801 gaacccacaa gccaaaactc tgatttcaac ccggctggaa gtggcaacac gaaagaaacg
   115861 tcgtgtgctt tttatttaag ccgcgcgccg cgttttatac cccacaatgg cagaaaattg
   115921 caaaagataa atacgcagaa tgccggcatt gtcaggaaaa atttccagcc acgtttttaa
   115981 cagaatgaga cacgattcaa aaaaaagtgg aaatcgggtg aagaattgac ctaaaatagc
   116041 catccagatg ttaatccatc cataccgatt aacactcaga ctgccagtgt ttttaacttg
   116101 cagagtcgtg gtaggatccg ctaccacaga aaatccacac aacagtttga gctaaccaaa
   116161 ttctctttag gtgatattaa atatggcaaa acaccttttt acgtccgagt ccgtctctga
   116221 agggcatcct gacaaaattg ctgaccaaat ctctgatgcc gttttagacg cgatcctcga
   116281 acaggatcca aaagcacgcg ttgcttgcga aacctacgta aaaaccggca tggttttagt
   116341 tggcggcgaa atcaccacca gcgcctgggt agacatcgaa gagatcaccc gtaacaccgt
   116401 tcgcgaaatt ggctatgtgc attccgacat gggctttgac gctaactcct gtgcggttct
   116461 gagcgctatc ggcaaacagt ctcctgacat caaccagggc gttgaccgtg ccgatccgct
   116521 ggaacagggc gcgggtgacc agggtctgat gtttggctac gcaactaatg aaaccgacgt
   116581 gctgatgcca gcacctatca cctatgcaca ccgtctggta cagcgtcagg ctgaagtgcg
   116641 taaaaacggc actctgccgt ggctgcgccc ggacgcgaaa agccaggtga ctttccagta
   116701 tgacgacggc aaaatcgttg gtatcgatgc tgtcgtgctt tccactcagc actctgaaga
   116761 gatcgaccag aaatcgctgc aagaagcggt aatggaagag atcatcaagc cgattctgcc
   116821 cgctgaatgg ctgacttctg ccaccaaatt cttcatcaac ccgaccggtc gttttgttat
   116881 cggtggcccg atgggtgact gcggtctgac tggtcgtaaa attatcgttg atacctacgg
   116941 cggcatggca cgtcacggtg gcggagcatt ctctggtaaa gatccatcaa aagtggaccg
   117001 ttccgctgcg tacgcagcac gttatgttgc aaaaaacatc gttgctgctg gcctggccga
   117061 tcgttgtgaa attcaggttt cctacgcaat cggcgtggct gaaccgacct ccatcatggt
   117121 agaaactttc ggtactgaga aagtgccttc tgaacaactg actctgctgg tacgtgagtt
   117181 cttcgacctg cgcccatacg gtctgattca gatgctggat ctgctgcacc cgatctacaa
   117241 agaaaccgca gcatacggtc actttggtcg tgaacatttc ccgtgggaaa aaaccgacaa
   117301 agcgcagctg ctgcgcgatg ctgccggtct gaagtaatct ttcttcacct gctttcaaag
   117361 gccagcctcg cgctggcctt tttcttttac ctcaccgcgg tggataggca ttcacgccgc
   117421 atccggcaaa aagctgcccg cacaataaca tcattcttcc tcaacacgtt ttatcgcaaa
   117481 ttatcatcac tactgaaacc gattacacca ataacaccaa ccaaatattt gtaatatttt
   117541 catattatta ttcagttttc acagttgtta catttctttt cagtaaagtc ttaattgcag
   117601 ataacagcgt ttaatctatg atgatataac tcaattattt tcatgcactt aaaccataac
   117661 taagataaat gttagtgtaa gcgattacac tgatgtgatt tgcttcacat ctttttacgt
   117721 cgtactcacc tatcttaatt cacaataaaa aataaccata ttggagggca tcatgcctga
   117781 cgctaaaaaa caggggcggt caaacaaggc aatgacgttt ttcgtctgct tccttgccgc
   117841 tctggcggga ttactctttg gcctggatat cggtgtaatt gctggcgcac tgccgtttat
   117901 tgcagatgaa ttccagatta cttcgcacac gcaagaatgg gtcgtaagct ccatgatgtt
   117961 cggtgcggca gtcggtgcgg tgggcagcgg ctggctctcc tttaaactcg ggcgcaaaaa
   118021 gagcctgatg atcggcgcaa ttttgtttgt tgccggttcg ctgttctctg cggctgcgcc
   118081 aaacgttgaa gtactgattc tttcccgcgt tctactgggg ctggcggtgg gtgtggcctc
   118141 ttataccgca ccgctgtacc tctctgaaat tgcgccggaa aaaattcgtg gcagtatgat
   118201 ctcgatgtat cagttgatga tcactatcgg gatcctcggt gcttatcttt ctgataccgc
   118261 cttcagctac accggtgcat ggcgctggat gctgggtgtg attatcatcc cggcaatttt
   118321 gctgctgatt ggtgtcttct tcctgccaga cagcccacgt tggtttgccg ccaaacgccg
   118381 ttttgttgat gccgaacgcg tgctgctacg cctgcgtgac accagcgcgg aagcgaaacg
   118441 cgaactggat gaaatccgtg aaagtttgca ggttaaacag agtggctggg cgctgtttaa
   118501 agagaacagc aacttccgcc gcgcggtgtt ccttggcgta ctgttgcagg taatgcagca
   118561 attcaccggg atgaacgtca tcatgtatta cgcgccgaaa atcttcgaac tggcgggtta
   118621 taccaacact accgagcaaa tgtgggggac cgtgattgtc ggcctgacca acgtacttgc
   118681 cacctttatc gcaatcggcc ttgttgaccg ctggggacgt aaaccaacgc taacgctggg
   118741 cttcctggtg atggctgctg gcatgggcgt actcggtaca atgatgcata taggtattca
   118801 ctctccgtca gcgcagtatt tcgccatcgc catgctgctg atgtttattg tcggttttgc
   118861 catgagtgcc ggtccgctga tttgggtact gtgctccgaa attcagccgc tgaaaggccg
   118921 cgattttggc atcacctgct ccaccgccac caactggatt gccaacatga tcgttggcgc
   118981 aacgttcctg accatgctca acacgctggg taacgccaac accttctggg tgtatgcggc
   119041 tctgaacgta ctgtttatcc tgctgacatt gtggctagta ccggaaacca aacacgtttc
   119101 gctggaacat attgaacgta atctgatgaa aggtcgtaaa ctgcgcgaaa tcggcgctca
   119161 cgattaatct cctcaggctt cctcccgtcg cggaggaagc cacctcttgc agtcatcttt
   119221 tcttcgttct atcctctgcc gctatgaaaa catcccgtct ccctatcgcc atccaacagg
   119281 ccgttatgcg ccgcctgcgg gaaaaactcg cccaggccaa cctgaagcta gggcgtaact
   119341 acccggagcc aaaactctct tacacccagc gcggaacctc cgccggaacg gcctggctgg
   119401 aaagctatga aattcgcctc aatcccgttt tgctgttgga aaacagtgaa gcttttattg
   119461 aagaagtggt accgcacgaa ctggcacatt tgctggtatg gaaacatttc ggccgcgtag
   119521 cgccacatgg caaagagtgg aagtggatga tggaaagcgt gctgggtgtt cccgcccgtc
   119581 gtacgcatca gttcgaactg caatccgtgc gtcgcaacac cttcccctac cgctgcaagt
   119641 gccaggagca tcagcttacc gtacgccgcc ataatcgcgt agttcgtggc gaggccgtct
   119701 atcgctgtgt tcactgcggt gaacagctgg ttgcgaaata accatctgaa ctatcaggaa
   119761 ctttcctgat ctggctgatt gcataccaaa acaactttcg ctacgttgct ggctcgtttt
   119821 aacacggagt aagtgatgta ccgttatttg tctattgctg cggtggtact gagcgcagca
   119881 ttttccggcc cggcgttggc cgaaggtatc aatagttttt ctcaggcgaa agccgcggcg
   119941 gtaaaagtcc acgctgacgc gcccggtacg ttttattgcg gatgtaaaat taactggcag
   120001 ggcaaaaaag gcgttgttga tctgcaatcg tgcggctatc aggtgcgcaa aaatgaaaac
   120061 cgagccagcc gcgtagagtg ggaacacgtc gttcccgcct ggcagttcgg tcaccagcgc
   120121 cagtgctggc aggacggtgg acgtaaaaac tgcgctaaag atccggtcta tcgcaagatg
   120181 gaaagcgata tgcataacct gcagccgtca gtcggtgagg tgaatggcga tcgcggcaac
   120241 tttatgtaca gccagtggaa tggcggcgaa ggccagtacg gtcaatgcgc catgaaggtc
   120301 gatttcaaag aaaaagctgc cgaaccacca gcgcgtgcac gcggtgccat tgcacgcacc
   120361 tacttctata tgcgcgacca atacaacctg acactctctc gccagcaaac gcagctgttc
   120421 aacgcatgga acaagatgta tccggttacc gactgggagt gcgagcgcga tgaacgcatc
   120481 gcgaaggtgc aaggcaatca taatccgtat gtgcaacgcg cttgccaggc gcgaaagagc
   120541 taacctacac tagcgggact ctttttgtta acccctaccc cacgcgtaca accgcgtggg
   120601 gagacgacgc ggatttttaa ctatgcgtat cccccgcatt tatcatcctg aaccactgac
   120661 cagccattct cacatcgcgc tttgcgaaga tgccgccaac catatcgggc gcgtactgcg
   120721 catggggccg gggcaggcgt tgcaattgtt tgacggtagc aaccaggtct ttgacgccga
   120781 aattaccagc gccagcaaaa aaagcgtgga agtgaaggtg ctggaaggca agatcgacga
   120841 tcgcgaatct ccgctgcata ttcacctcgg tcaggtgatg tcgcgtggtg aaaaaatgga
   120901 atttacgatc cagaaatcga tcgaactcgg tgtaagcctc attacgccac ttttttctga
   120961 gcgctgcggc gttaaactgg atagtgaacg tctgaacaag aagctccagc agtggcagaa
   121021 gattgcaatt gctgcctgtg agcagtgtgg tcgtaaccgg gtgccggaaa tccgcccagc
   121081 gatggatctg gaagcctggt gtgcagagca ggatgaagga ctgaaactga atcttcaccc
   121141 gcgcgccagt aacagcatca atacgttgcc gttaccggtt gaacgcgtcc gcctgctgat
   121201 tggcccggaa ggcggtttat cggcagatga aattgccatg actgcccgct atcaatttac
   121261 tgatatcctg ttgggacctc gcgttttgcg tacagagaca actgcgctca ccgccattac
   121321 cgcgctacaa gtacgatttg gcgatttggg ctaacggaga agaataatga tcaagctcgg
   121381 catcgtgatg gaccccatcg caaacatcaa catcaagaaa gattccagtt tcgctatgtt
   121441 gctggaagca cagcgtcgtg gttacgaact tcactatatg gagatgggcg atctgtatct
   121501 gatcaatggc gaagcccgcg cccatacccg cacgctgaac gtgaagcaga actacgaaga
   121561 gtggttttca ttcgtcggtg aacaggatct gccgctggcc gatctcgatg tgatcctgat
   121621 gcgtaaagac ccgccgtttg ataccgagtt tatctacgcg acctatattc tggaacgtgc
   121681 cgaagagaaa gggacgctga tcgttaacaa gccgcagagc ctgcgcgact gtaacgagaa
   121741 actgtttacc gcctggttct ctgacttaac gccagaaacg ctggttacgc gcaataaagc
   121801 gcagctaaaa gcgttctggg agaaacacag cgacatcatt cttaagccgc tggacggtat
   121861 gggcggcgcg tcgattttcc gcgtgaaaga aggcgatcca aacctcggcg tgattgccga
   121921 aaccctgact gagcatggca ctcgctactg catggcgcaa aattacctgc cagccattaa
   121981 agatggcgac aaacgcgtgc tggtggtgga tggcgagccg gtaccgtact gcctggcgcg
   122041 tattccgcag gggggcgaaa cccgtggcaa tctggctgcc ggtggtcgcg gtgaacctcg
   122101 tccgctgacg gaaagtgact ggaaaatcgc ccgtcagatc gggccgacgc tgaaagaaaa
   122161 agggctgatt tttgttggtc tggatatcat cggcgaccgt ctgactgaaa ttaacgtcac
   122221 cagcccaacc tgtattcgtg agattgaagc agagtttccg gtgtcgatca ccggaatgtt
   122281 aatggatgcc atcgaagcac gtttacagca gcagtaaccc accttagcga gaaggatctc
   122341 gttgagactc tgagtgacag cgcccttctt tccacgcata ctgggcgctg ttgctttttt
   122401 gaaccaggaa acagaacctc tgacaatgaa tttacagcat cactttctta ttgccatgcc
   122461 tgctctccag gatccgattt tccgtcgttc cgtggtctac atttgcgaac ataataccaa
   122521 tggtgcaatg gggatcatcg tcaacaagcc gctggaaaat ctcaaaattg aagggattct
   122581 ggaaaagctg aagatcacgc cggagccgcg tgatgaatca atccgtctgg ataaaccggt
   122641 tatgctcggc ggtccgctgg ctgaagatcg cgggtttatt ttgcatactc cgccctccaa
   122701 ttttgcttcc agcattcgca tttcagacaa cacagtgatg accacttccc gcgatgtgct
   122761 ggaaacgctc ggcaccgata aacaaccgtc tgacgtattg gtggctctgg gttatgcctc
   122821 ctgggaaaaa ggtcaactgg aacaagaaat tctcgataac gcgtggctaa cggccccggc
   122881 ggatctgaat attctgttca aaacgccgat tgccgaccgc tggcgcgagg cggcaaaact
   122941 gattggtgtg gatattctca ccatgcctgg tgtggcaggg cacgcctgat gagtggaacc
   123001 ttactcgcct tcgacttcgg caccaaaagc attggcgtag cggtcggcca acgcattacc
   123061 ggcactgctc gccctttgcc tgcaattaaa gcacaggacg gtacgccgga ctggaacctc
   123121 atcgaacgtt tactgaaaga gtggcagccg gacgaaatca tcgtcggttt gccgctgaat
   123181 atggacggca ccgagcaacc attgactgcc agagcgcgta aatttgccaa ccgtattcat
   123241 ggtcgtttcg gtgttgaagt aaagctccat gacgaacgtc ttagcaccgt ggaagcccgt
   123301 tccggtctgt ttgaacaggg cggctatcgg gcgctcaaca aaggcaaagt cgactctgcc
   123361 tctgcggtta ttattctcga aagctatttc gagcagggat attaaggcga tctaaaagcc
   123421 tggcggatag tcgttaccgc catcttgccg gatgcggcgt gaacgcctta tccagtacac
   123481 atctggcagc ggctgtaggt ctgataagac gcgttagcgt cgcatcagac atttattgcc
   123541 ttt
//
LOCUS       DAZGAN020000002       101222 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGAN020000002 DAZGAN020000000
VERSION     DAZGAN020000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 101222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 101222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 101222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.002"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..758
                     /locus_tag="ACHKYO_RS00635"
                     /old_locus_tag="pgaptmp_000127"
     CDS             <1..758
                     /locus_tag="ACHKYO_RS00635"
                     /old_locus_tag="pgaptmp_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HGU4427034.1"
                     /translation="VAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAMNYRGFT
                     FPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTL
                     LGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQDTALFAL
                     YQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEVVLPASP
                     FLNVAQWQRKEGHGQLTNGILALPAISATVWMN"
     gene            complement(763..1344)
                     /gene="acpH"
                     /locus_tag="ACHKYO_RS00640"
                     /old_locus_tag="pgaptmp_000128"
     CDS             complement(763..1344)
                     /gene="acpH"
                     /locus_tag="ACHKYO_RS00640"
                     /old_locus_tag="pgaptmp_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HGU4427035.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            1437..2507
                     /gene="queA"
                     /locus_tag="ACHKYO_RS00645"
                     /old_locus_tag="pgaptmp_000129"
     CDS             1437..2507
                     /gene="queA"
                     /locus_tag="ACHKYO_RS00645"
                     /old_locus_tag="pgaptmp_000129"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGU4427036.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            2563..3690
                     /gene="tgt"
                     /locus_tag="ACHKYO_RS00650"
                     /old_locus_tag="pgaptmp_000130"
     CDS             2563..3690
                     /gene="tgt"
                     /locus_tag="ACHKYO_RS00650"
                     /old_locus_tag="pgaptmp_000130"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGU4427037.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            3713..4045
                     /gene="yajC"
                     /locus_tag="ACHKYO_RS00655"
                     /old_locus_tag="pgaptmp_000131"
     CDS             3713..4045
                     /gene="yajC"
                     /locus_tag="ACHKYO_RS00655"
                     /old_locus_tag="pgaptmp_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGU4427038.1"
                     /translation="MSFFISDAVAATGAPAQGSSMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            4073..5920
                     /gene="secD"
                     /locus_tag="ACHKYO_RS00660"
                     /old_locus_tag="pgaptmp_000132"
     CDS             4073..5920
                     /gene="secD"
                     /locus_tag="ACHKYO_RS00660"
                     /old_locus_tag="pgaptmp_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HGU4427039.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            5931..6902
                     /gene="secF"
                     /locus_tag="ACHKYO_RS00665"
                     /old_locus_tag="pgaptmp_000133"
     CDS             5931..6902
                     /gene="secF"
                     /locus_tag="ACHKYO_RS00665"
                     /old_locus_tag="pgaptmp_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HGU4427040.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            7031..7378
                     /gene="yajD"
                     /locus_tag="ACHKYO_RS00670"
                     /old_locus_tag="pgaptmp_000134"
     CDS             7031..7378
                     /gene="yajD"
                     /locus_tag="ACHKYO_RS00670"
                     /old_locus_tag="pgaptmp_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HGU4427041.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(7555..8439)
                     /gene="tsx"
                     /locus_tag="ACHKYO_RS00675"
                     /old_locus_tag="pgaptmp_000135"
     CDS             complement(7555..8439)
                     /gene="tsx"
                     /locus_tag="ACHKYO_RS00675"
                     /old_locus_tag="pgaptmp_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HGU4427042.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(8738..9277)
                     /gene="yajI"
                     /locus_tag="ACHKYO_RS00680"
                     /old_locus_tag="pgaptmp_000136"
     CDS             complement(8738..9277)
                     /gene="yajI"
                     /locus_tag="ACHKYO_RS00680"
                     /old_locus_tag="pgaptmp_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU4427043.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            9428..9877
                     /gene="nrdR"
                     /locus_tag="ACHKYO_RS00685"
                     /old_locus_tag="pgaptmp_000137"
     CDS             9428..9877
                     /gene="nrdR"
                     /locus_tag="ACHKYO_RS00685"
                     /old_locus_tag="pgaptmp_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGU4427044.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            9881..10984
                     /gene="ribD"
                     /locus_tag="ACHKYO_RS00690"
                     /old_locus_tag="pgaptmp_000138"
     CDS             9881..10984
                     /gene="ribD"
                     /locus_tag="ACHKYO_RS00690"
                     /old_locus_tag="pgaptmp_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGU4427045.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQVGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            11073..11543
                     /gene="ribE"
                     /locus_tag="ACHKYO_RS00695"
                     /old_locus_tag="pgaptmp_000139"
     CDS             11073..11543
                     /gene="ribE"
                     /locus_tag="ACHKYO_RS00695"
                     /old_locus_tag="pgaptmp_000139"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGU4427046.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            11563..11982
                     /gene="nusB"
                     /locus_tag="ACHKYO_RS00700"
                     /old_locus_tag="pgaptmp_000140"
     CDS             11563..11982
                     /gene="nusB"
                     /locus_tag="ACHKYO_RS00700"
                     /old_locus_tag="pgaptmp_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGU4427047.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            12060..13037
                     /gene="thiL"
                     /locus_tag="ACHKYO_RS00705"
                     /old_locus_tag="pgaptmp_000141"
     CDS             12060..13037
                     /gene="thiL"
                     /locus_tag="ACHKYO_RS00705"
                     /old_locus_tag="pgaptmp_000141"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HGU4427048.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            13015..13533
                     /gene="pgpA"
                     /locus_tag="ACHKYO_RS00710"
                     /old_locus_tag="pgaptmp_000142"
     CDS             13015..13533
                     /gene="pgpA"
                     /locus_tag="ACHKYO_RS00710"
                     /old_locus_tag="pgaptmp_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HGU4427049.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(13587..14561)
                     /gene="yajO"
                     /locus_tag="ACHKYO_RS00715"
                     /old_locus_tag="pgaptmp_000143"
     CDS             complement(13587..14561)
                     /gene="yajO"
                     /locus_tag="ACHKYO_RS00715"
                     /old_locus_tag="pgaptmp_000143"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HGU4427050.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(14741..16603)
                     /gene="dxs"
                     /locus_tag="ACHKYO_RS00720"
                     /old_locus_tag="pgaptmp_000144"
     CDS             complement(14741..16603)
                     /gene="dxs"
                     /locus_tag="ACHKYO_RS00720"
                     /old_locus_tag="pgaptmp_000144"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HGU4427051.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(16628..17527)
                     /gene="ispA"
                     /locus_tag="ACHKYO_RS00725"
                     /old_locus_tag="pgaptmp_000145"
     CDS             complement(16628..17527)
                     /gene="ispA"
                     /locus_tag="ACHKYO_RS00725"
                     /old_locus_tag="pgaptmp_000145"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HGU4427052.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(17527..17769)
                     /gene="xseB"
                     /locus_tag="ACHKYO_RS00730"
                     /old_locus_tag="pgaptmp_000146"
     CDS             complement(17527..17769)
                     /gene="xseB"
                     /locus_tag="ACHKYO_RS00730"
                     /old_locus_tag="pgaptmp_000146"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGU4427053.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            17975..19423
                     /gene="thiI"
                     /locus_tag="ACHKYO_RS00735"
                     /old_locus_tag="pgaptmp_000147"
     CDS             17975..19423
                     /gene="thiI"
                     /locus_tag="ACHKYO_RS00735"
                     /old_locus_tag="pgaptmp_000147"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGU4427054.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(19477..20067)
                     /gene="yajL"
                     /locus_tag="ACHKYO_RS00740"
                     /old_locus_tag="pgaptmp_000148"
     CDS             complement(19477..20067)
                     /gene="yajL"
                     /locus_tag="ACHKYO_RS00740"
                     /old_locus_tag="pgaptmp_000148"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HGU4427055.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(20030..20941)
                     /gene="panE"
                     /locus_tag="ACHKYO_RS00745"
                     /old_locus_tag="pgaptmp_000149"
     CDS             complement(20030..20941)
                     /gene="panE"
                     /locus_tag="ACHKYO_RS00745"
                     /old_locus_tag="pgaptmp_000149"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HGU4427056.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            21109..21600
                     /gene="yajQ"
                     /locus_tag="ACHKYO_RS00750"
                     /old_locus_tag="pgaptmp_000150"
     CDS             21109..21600
                     /gene="yajQ"
                     /locus_tag="ACHKYO_RS00750"
                     /old_locus_tag="pgaptmp_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HGU4427057.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(21728..23092)
                     /gene="yajR"
                     /locus_tag="ACHKYO_RS00755"
                     /old_locus_tag="pgaptmp_000151"
     CDS             complement(21728..23092)
                     /gene="yajR"
                     /locus_tag="ACHKYO_RS00755"
                     /old_locus_tag="pgaptmp_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4427058.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            23500..24434
                     /locus_tag="ACHKYO_RS00760"
                     /old_locus_tag="pgaptmp_000152"
                     /pseudo
     CDS             23500..24434
                     /locus_tag="ACHKYO_RS00760"
                     /old_locus_tag="pgaptmp_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(24490..25032)
                     /locus_tag="ACHKYO_RS00765"
                     /old_locus_tag="pgaptmp_000153"
     CDS             complement(24490..25032)
                     /locus_tag="ACHKYO_RS00765"
                     /old_locus_tag="pgaptmp_000153"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4427059.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(25016..25327)
                     /locus_tag="ACHKYO_RS00770"
                     /old_locus_tag="pgaptmp_000154"
     CDS             complement(25016..25327)
                     /locus_tag="ACHKYO_RS00770"
                     /old_locus_tag="pgaptmp_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HGU4427060.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(25512..26402)
                     /gene="cyoE"
                     /locus_tag="ACHKYO_RS00775"
                     /old_locus_tag="pgaptmp_000155"
     CDS             complement(25512..26402)
                     /gene="cyoE"
                     /locus_tag="ACHKYO_RS00775"
                     /old_locus_tag="pgaptmp_000155"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HGU4427061.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(26414..26743)
                     /gene="cyoD"
                     /locus_tag="ACHKYO_RS00780"
                     /old_locus_tag="pgaptmp_000156"
     CDS             complement(26414..26743)
                     /gene="cyoD"
                     /locus_tag="ACHKYO_RS00780"
                     /old_locus_tag="pgaptmp_000156"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HGU4427062.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(26743..27357)
                     /gene="cyoC"
                     /locus_tag="ACHKYO_RS00785"
                     /old_locus_tag="pgaptmp_000157"
     CDS             complement(26743..27357)
                     /gene="cyoC"
                     /locus_tag="ACHKYO_RS00785"
                     /old_locus_tag="pgaptmp_000157"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HGU4427063.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(27347..29338)
                     /gene="cyoB"
                     /locus_tag="ACHKYO_RS00790"
                     /old_locus_tag="pgaptmp_000158"
     CDS             complement(27347..29338)
                     /gene="cyoB"
                     /locus_tag="ACHKYO_RS00790"
                     /old_locus_tag="pgaptmp_000158"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HGU4427064.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(29360..30307)
                     /gene="cyoA"
                     /locus_tag="ACHKYO_RS00795"
                     /old_locus_tag="pgaptmp_000159"
     CDS             complement(29360..30307)
                     /gene="cyoA"
                     /locus_tag="ACHKYO_RS00795"
                     /old_locus_tag="pgaptmp_000159"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HGU4427065.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(30769..32244)
                     /gene="ampG"
                     /locus_tag="ACHKYO_RS00800"
                     /old_locus_tag="pgaptmp_000160"
     CDS             complement(30769..32244)
                     /gene="ampG"
                     /locus_tag="ACHKYO_RS00800"
                     /old_locus_tag="pgaptmp_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HGU4427066.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(32288..32866)
                     /gene="yajG"
                     /locus_tag="ACHKYO_RS00805"
                     /old_locus_tag="pgaptmp_000161"
     CDS             complement(32288..32866)
                     /gene="yajG"
                     /locus_tag="ACHKYO_RS00805"
                     /old_locus_tag="pgaptmp_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4427067.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            33171..33488
                     /gene="bolA"
                     /locus_tag="ACHKYO_RS00810"
                     /old_locus_tag="pgaptmp_000162"
     CDS             33171..33488
                     /gene="bolA"
                     /locus_tag="ACHKYO_RS00810"
                     /old_locus_tag="pgaptmp_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HGU4427068.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            33832..35130
                     /gene="tig"
                     /locus_tag="ACHKYO_RS00815"
                     /old_locus_tag="pgaptmp_000163"
     CDS             33832..35130
                     /gene="tig"
                     /locus_tag="ACHKYO_RS00815"
                     /old_locus_tag="pgaptmp_000163"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGU4427069.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            35376..35999
                     /gene="clpP"
                     /locus_tag="ACHKYO_RS00820"
                     /old_locus_tag="pgaptmp_000164"
     CDS             35376..35999
                     /gene="clpP"
                     /locus_tag="ACHKYO_RS00820"
                     /old_locus_tag="pgaptmp_000164"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGU4427070.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            36125..37399
                     /gene="clpX"
                     /locus_tag="ACHKYO_RS00825"
                     /old_locus_tag="pgaptmp_000165"
     CDS             36125..37399
                     /gene="clpX"
                     /locus_tag="ACHKYO_RS00825"
                     /old_locus_tag="pgaptmp_000165"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HGU4427071.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            37587..39941
                     /gene="lon"
                     /locus_tag="ACHKYO_RS00830"
                     /old_locus_tag="pgaptmp_000166"
     CDS             37587..39941
                     /gene="lon"
                     /locus_tag="ACHKYO_RS00830"
                     /old_locus_tag="pgaptmp_000166"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HGU4427072.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            40132..40422
                     /gene="hupB"
                     /locus_tag="ACHKYO_RS00835"
                     /old_locus_tag="pgaptmp_000167"
     CDS             40132..40422
                     /gene="hupB"
                     /locus_tag="ACHKYO_RS00835"
                     /old_locus_tag="pgaptmp_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HGU4427073.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            40614..42485
                     /gene="ppiD"
                     /locus_tag="ACHKYO_RS00840"
                     /old_locus_tag="pgaptmp_000168"
     CDS             40614..42485
                     /gene="ppiD"
                     /locus_tag="ACHKYO_RS00840"
                     /old_locus_tag="pgaptmp_000168"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU4427074.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAQQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            42636..43007
                     /gene="ybaV"
                     /locus_tag="ACHKYO_RS00845"
                     /old_locus_tag="pgaptmp_000169"
     CDS             42636..43007
                     /gene="ybaV"
                     /locus_tag="ACHKYO_RS00845"
                     /old_locus_tag="pgaptmp_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGU4427075.1"
                     /translation="MKHGIKALLITLSLACAGMSYSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            43101..43499
                     /gene="fadM"
                     /locus_tag="ACHKYO_RS00850"
                     /old_locus_tag="pgaptmp_000170"
     CDS             43101..43499
                     /gene="fadM"
                     /locus_tag="ACHKYO_RS00850"
                     /old_locus_tag="pgaptmp_000170"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HGU4427076.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(43551..44246)
                     /gene="queC"
                     /locus_tag="ACHKYO_RS00855"
                     /old_locus_tag="pgaptmp_000171"
     CDS             complement(43551..44246)
                     /gene="queC"
                     /locus_tag="ACHKYO_RS00855"
                     /old_locus_tag="pgaptmp_000171"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HGU4427077.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(44311..46011)
                     /gene="ybaE"
                     /locus_tag="ACHKYO_RS00860"
                     /old_locus_tag="pgaptmp_000172"
     CDS             complement(44311..46011)
                     /gene="ybaE"
                     /locus_tag="ACHKYO_RS00860"
                     /old_locus_tag="pgaptmp_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HGU4427078.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            46111..46929
                     /gene="cof"
                     /locus_tag="ACHKYO_RS00865"
                     /old_locus_tag="pgaptmp_000173"
     CDS             46111..46929
                     /gene="cof"
                     /locus_tag="ACHKYO_RS00865"
                     /old_locus_tag="pgaptmp_000173"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HGU4427079.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            47082..47540
                     /gene="decR"
                     /locus_tag="ACHKYO_RS00870"
                     /old_locus_tag="pgaptmp_000174"
     CDS             47082..47540
                     /gene="decR"
                     /locus_tag="ACHKYO_RS00870"
                     /old_locus_tag="pgaptmp_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HGU4427080.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            47570..49342
                     /gene="mdlA"
                     /locus_tag="ACHKYO_RS00875"
                     /old_locus_tag="pgaptmp_000175"
     CDS             47570..49342
                     /gene="mdlA"
                     /locus_tag="ACHKYO_RS00875"
                     /old_locus_tag="pgaptmp_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU4427081.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVAQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWEQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            49335..51116
                     /gene="mdlB"
                     /locus_tag="ACHKYO_RS00880"
                     /old_locus_tag="pgaptmp_000176"
     CDS             49335..51116
                     /gene="mdlB"
                     /locus_tag="ACHKYO_RS00880"
                     /old_locus_tag="pgaptmp_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU4427082.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDER
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            51297..51635
                     /gene="glnK"
                     /locus_tag="ACHKYO_RS00885"
                     /old_locus_tag="pgaptmp_000177"
     CDS             51297..51635
                     /gene="glnK"
                     /locus_tag="ACHKYO_RS00885"
                     /old_locus_tag="pgaptmp_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HGU4427083.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            51665..52951
                     /gene="amtB"
                     /locus_tag="ACHKYO_RS00890"
                     /old_locus_tag="pgaptmp_000178"
     CDS             51665..52951
                     /gene="amtB"
                     /locus_tag="ACHKYO_RS00890"
                     /old_locus_tag="pgaptmp_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HGU4427084.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(52999..53859)
                     /gene="tesB"
                     /locus_tag="ACHKYO_RS00895"
                     /old_locus_tag="pgaptmp_000179"
     CDS             complement(52999..53859)
                     /gene="tesB"
                     /locus_tag="ACHKYO_RS00895"
                     /old_locus_tag="pgaptmp_000179"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HGU4427085.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            54077..54649
                     /gene="ybaY"
                     /locus_tag="ACHKYO_RS00900"
                     /old_locus_tag="pgaptmp_000180"
     CDS             54077..54649
                     /gene="ybaY"
                     /locus_tag="ACHKYO_RS00900"
                     /old_locus_tag="pgaptmp_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HGU4427086.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPVPTQY"
     gene            complement(54680..54991)
                     /locus_tag="ACHKYO_RS00905"
                     /old_locus_tag="pgaptmp_000181"
     CDS             complement(54680..54991)
                     /locus_tag="ACHKYO_RS00905"
                     /old_locus_tag="pgaptmp_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HGU4427087.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            55154..55250
                     /gene="ffs"
                     /locus_tag="ACHKYO_RS00910"
                     /old_locus_tag="pgaptmp_000182"
     ncRNA           55154..55250
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKYO_RS00910"
                     /old_locus_tag="pgaptmp_000182"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            55370..55723
                     /gene="ybaA"
                     /locus_tag="ACHKYO_RS00915"
                     /old_locus_tag="pgaptmp_000183"
     CDS             55370..55723
                     /gene="ybaA"
                     /locus_tag="ACHKYO_RS00915"
                     /old_locus_tag="pgaptmp_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HGU4427088.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAATQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(55765..57315)
                     /gene="pdeB"
                     /locus_tag="ACHKYO_RS00920"
                     /old_locus_tag="pgaptmp_000184"
     CDS             complement(55765..57315)
                     /gene="pdeB"
                     /locus_tag="ACHKYO_RS00920"
                     /old_locus_tag="pgaptmp_000184"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HGU4427089.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(57479..57949)
                     /gene="ylaC"
                     /locus_tag="ACHKYO_RS00925"
                     /old_locus_tag="pgaptmp_000185"
     CDS             complement(57479..57949)
                     /gene="ylaC"
                     /locus_tag="ACHKYO_RS00925"
                     /old_locus_tag="pgaptmp_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HGU4427090.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(58065..58616)
                     /gene="maa"
                     /locus_tag="ACHKYO_RS00930"
                     /old_locus_tag="pgaptmp_000186"
     CDS             complement(58065..58616)
                     /gene="maa"
                     /locus_tag="ACHKYO_RS00930"
                     /old_locus_tag="pgaptmp_000186"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HGU4427091.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(58788..59006)
                     /gene="hha"
                     /locus_tag="ACHKYO_RS00935"
                     /old_locus_tag="pgaptmp_000187"
     CDS             complement(58788..59006)
                     /gene="hha"
                     /locus_tag="ACHKYO_RS00935"
                     /old_locus_tag="pgaptmp_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HGU4427092.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(59032..59406)
                     /gene="tomB"
                     /locus_tag="ACHKYO_RS00940"
                     /old_locus_tag="pgaptmp_000188"
     CDS             complement(59032..59406)
                     /gene="tomB"
                     /locus_tag="ACHKYO_RS00940"
                     /old_locus_tag="pgaptmp_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HGU4427093.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(59952..63101)
                     /gene="acrB"
                     /locus_tag="ACHKYO_RS00945"
                     /old_locus_tag="pgaptmp_000189"
     CDS             complement(59952..63101)
                     /gene="acrB"
                     /locus_tag="ACHKYO_RS00945"
                     /old_locus_tag="pgaptmp_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HGU4427094.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(63124..64317)
                     /gene="acrA"
                     /locus_tag="ACHKYO_RS00950"
                     /old_locus_tag="pgaptmp_000190"
     CDS             complement(63124..64317)
                     /gene="acrA"
                     /locus_tag="ACHKYO_RS00950"
                     /old_locus_tag="pgaptmp_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HGU4427095.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            64459..65106
                     /gene="acrR"
                     /locus_tag="ACHKYO_RS00955"
                     /old_locus_tag="pgaptmp_000191"
     CDS             64459..65106
                     /gene="acrR"
                     /locus_tag="ACHKYO_RS00955"
                     /old_locus_tag="pgaptmp_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HGU4427096.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            65234..68596
                     /gene="mscK"
                     /locus_tag="ACHKYO_RS00960"
                     /old_locus_tag="pgaptmp_000192"
     CDS             65234..68596
                     /gene="mscK"
                     /locus_tag="ACHKYO_RS00960"
                     /old_locus_tag="pgaptmp_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HGU4427097.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWCRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            68657..68755
                     /locus_tag="ACHKYO_RS00965"
                     /old_locus_tag="pgaptmp_000193"
                     /pseudo
     CDS             68657..68755
                     /locus_tag="ACHKYO_RS00965"
                     /old_locus_tag="pgaptmp_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(68808..68969)
                     /gene="rsmS"
                     /locus_tag="ACHKYO_RS00970"
                     /old_locus_tag="pgaptmp_000194"
     CDS             complement(68808..68969)
                     /gene="rsmS"
                     /locus_tag="ACHKYO_RS00970"
                     /old_locus_tag="pgaptmp_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HGU4427098.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(68983..69510)
                     /gene="priC"
                     /locus_tag="ACHKYO_RS00975"
                     /old_locus_tag="pgaptmp_000195"
     CDS             complement(68983..69510)
                     /gene="priC"
                     /locus_tag="ACHKYO_RS00975"
                     /old_locus_tag="pgaptmp_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HGU4427099.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            69580..69957
                     /gene="ybaN"
                     /locus_tag="ACHKYO_RS00980"
                     /old_locus_tag="pgaptmp_000196"
     CDS             69580..69957
                     /gene="ybaN"
                     /locus_tag="ACHKYO_RS00980"
                     /old_locus_tag="pgaptmp_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HGU4427100.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            70110..70661
                     /gene="apt"
                     /locus_tag="ACHKYO_RS00985"
                     /old_locus_tag="pgaptmp_000197"
     CDS             70110..70661
                     /gene="apt"
                     /locus_tag="ACHKYO_RS00985"
                     /old_locus_tag="pgaptmp_000197"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGU4427101.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            70790..72721
                     /gene="dnaX"
                     /locus_tag="ACHKYO_RS00990"
                     /old_locus_tag="pgaptmp_000198"
     CDS             70790..72721
                     /gene="dnaX"
                     /locus_tag="ACHKYO_RS00990"
                     /old_locus_tag="pgaptmp_000198"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGU4427102.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    72054..72118
                     /gene="dnaX"
                     /locus_tag="ACHKYO_RS00990"
                     /old_locus_tag="pgaptmp_000198"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            72774..73103
                     /gene="ybaB"
                     /locus_tag="ACHKYO_RS00995"
                     /old_locus_tag="pgaptmp_000199"
     CDS             72774..73103
                     /gene="ybaB"
                     /locus_tag="ACHKYO_RS00995"
                     /old_locus_tag="pgaptmp_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGU4427103.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            73103..73708
                     /gene="recR"
                     /locus_tag="ACHKYO_RS01000"
                     /old_locus_tag="pgaptmp_000200"
     CDS             73103..73708
                     /gene="recR"
                     /locus_tag="ACHKYO_RS01000"
                     /old_locus_tag="pgaptmp_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGU4427104.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            73818..75692
                     /gene="htpG"
                     /locus_tag="ACHKYO_RS01005"
                     /old_locus_tag="pgaptmp_000201"
     CDS             73818..75692
                     /gene="htpG"
                     /locus_tag="ACHKYO_RS01005"
                     /old_locus_tag="pgaptmp_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HGU4427105.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            75873..76517
                     /gene="adk"
                     /locus_tag="ACHKYO_RS01010"
                     /old_locus_tag="pgaptmp_000202"
     CDS             75873..76517
                     /gene="adk"
                     /locus_tag="ACHKYO_RS01010"
                     /old_locus_tag="pgaptmp_000202"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGU4427106.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            76753..77715
                     /gene="hemH"
                     /locus_tag="ACHKYO_RS01015"
                     /old_locus_tag="pgaptmp_000203"
     CDS             76753..77715
                     /gene="hemH"
                     /locus_tag="ACHKYO_RS01015"
                     /old_locus_tag="pgaptmp_000203"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGU4427107.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(77712..78671)
                     /gene="aes"
                     /locus_tag="ACHKYO_RS01020"
                     /old_locus_tag="pgaptmp_000204"
     CDS             complement(77712..78671)
                     /gene="aes"
                     /locus_tag="ACHKYO_RS01020"
                     /old_locus_tag="pgaptmp_000204"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HGU4427108.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQSDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            78823..80127
                     /gene="gsk"
                     /locus_tag="ACHKYO_RS01025"
                     /old_locus_tag="pgaptmp_000205"
     CDS             78823..80127
                     /gene="gsk"
                     /locus_tag="ACHKYO_RS01025"
                     /old_locus_tag="pgaptmp_000205"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HGU4427109.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(80260..81936)
                     /gene="ybaL"
                     /locus_tag="ACHKYO_RS01030"
                     /old_locus_tag="pgaptmp_000206"
     CDS             complement(80260..81936)
                     /gene="ybaL"
                     /locus_tag="ACHKYO_RS01030"
                     /old_locus_tag="pgaptmp_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HGU4427110.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(82174..83394)
                     /gene="fsr"
                     /locus_tag="ACHKYO_RS01035"
                     /old_locus_tag="pgaptmp_000207"
     CDS             complement(82174..83394)
                     /gene="fsr"
                     /locus_tag="ACHKYO_RS01035"
                     /old_locus_tag="pgaptmp_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HGU4427111.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDRYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            83612..85264
                     /gene="ushA"
                     /locus_tag="ACHKYO_RS01040"
                     /old_locus_tag="pgaptmp_000208"
     CDS             83612..85264
                     /gene="ushA"
                     /locus_tag="ACHKYO_RS01040"
                     /old_locus_tag="pgaptmp_000208"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HGU4427112.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(85301..85780)
                     /gene="ybaK"
                     /locus_tag="ACHKYO_RS01045"
                     /old_locus_tag="pgaptmp_000209"
     CDS             complement(85301..85780)
                     /gene="ybaK"
                     /locus_tag="ACHKYO_RS01045"
                     /old_locus_tag="pgaptmp_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HGU4427113.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(85984..86778)
                     /gene="ybaP"
                     /locus_tag="ACHKYO_RS01050"
                     /old_locus_tag="pgaptmp_000210"
     CDS             complement(85984..86778)
                     /gene="ybaP"
                     /locus_tag="ACHKYO_RS01050"
                     /old_locus_tag="pgaptmp_000210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HGU4427114.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            86916..87257
                     /gene="ybaQ"
                     /locus_tag="ACHKYO_RS01055"
                     /old_locus_tag="pgaptmp_000211"
     CDS             86916..87257
                     /gene="ybaQ"
                     /locus_tag="ACHKYO_RS01055"
                     /old_locus_tag="pgaptmp_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4427115.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(87371..89875)
                     /gene="copA"
                     /locus_tag="ACHKYO_RS01060"
                     /old_locus_tag="pgaptmp_000212"
     CDS             complement(87371..89875)
                     /gene="copA"
                     /locus_tag="ACHKYO_RS01060"
                     /old_locus_tag="pgaptmp_000212"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HGU4427116.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            90137..91069
                     /gene="glsA"
                     /locus_tag="ACHKYO_RS01065"
                     /old_locus_tag="pgaptmp_000213"
     CDS             90137..91069
                     /gene="glsA"
                     /locus_tag="ACHKYO_RS01065"
                     /old_locus_tag="pgaptmp_000213"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HGU4427117.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            91072..92364
                     /gene="ybaT"
                     /locus_tag="ACHKYO_RS01070"
                     /old_locus_tag="pgaptmp_000214"
     CDS             91072..92364
                     /gene="ybaT"
                     /locus_tag="ACHKYO_RS01070"
                     /old_locus_tag="pgaptmp_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU4427118.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            92395..92492
                     /locus_tag="ACHKYO_RS01075"
                     /old_locus_tag="pgaptmp_000215"
                     /pseudo
     CDS             92395..92492
                     /locus_tag="ACHKYO_RS01075"
                     /old_locus_tag="pgaptmp_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            92489..92896
                     /gene="cueR"
                     /locus_tag="ACHKYO_RS01080"
                     /old_locus_tag="pgaptmp_000216"
     CDS             92489..92896
                     /gene="cueR"
                     /locus_tag="ACHKYO_RS01080"
                     /old_locus_tag="pgaptmp_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HGU4427119.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(92897..93355)
                     /gene="ybbJ"
                     /locus_tag="ACHKYO_RS01085"
                     /old_locus_tag="pgaptmp_000217"
     CDS             complement(92897..93355)
                     /gene="ybbJ"
                     /locus_tag="ACHKYO_RS01085"
                     /old_locus_tag="pgaptmp_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HGU4427120.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVSAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(93352..94269)
                     /gene="qmcA"
                     /locus_tag="ACHKYO_RS01090"
                     /old_locus_tag="pgaptmp_000218"
     CDS             complement(93352..94269)
                     /gene="qmcA"
                     /locus_tag="ACHKYO_RS01090"
                     /old_locus_tag="pgaptmp_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGU4427121.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            94415..95092
                     /gene="fetA"
                     /locus_tag="ACHKYO_RS01095"
                     /old_locus_tag="pgaptmp_000219"
     CDS             94415..95092
                     /gene="fetA"
                     /locus_tag="ACHKYO_RS01095"
                     /old_locus_tag="pgaptmp_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HGU4427122.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            95079..95858
                     /gene="fetB"
                     /locus_tag="ACHKYO_RS01100"
                     /old_locus_tag="pgaptmp_000220"
     CDS             95079..95858
                     /gene="fetB"
                     /locus_tag="ACHKYO_RS01100"
                     /old_locus_tag="pgaptmp_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HGU4427123.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(95921..96775)
                     /gene="cnoX"
                     /locus_tag="ACHKYO_RS01105"
                     /old_locus_tag="pgaptmp_000221"
     CDS             complement(95921..96775)
                     /gene="cnoX"
                     /locus_tag="ACHKYO_RS01105"
                     /old_locus_tag="pgaptmp_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HGU4427124.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQESNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(96836..97645)
                     /gene="ybbO"
                     /locus_tag="ACHKYO_RS01110"
                     /old_locus_tag="pgaptmp_000222"
     CDS             complement(96836..97645)
                     /gene="ybbO"
                     /locus_tag="ACHKYO_RS01110"
                     /old_locus_tag="pgaptmp_000222"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU4427125.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(97635..98258)
                     /gene="tesA"
                     /locus_tag="ACHKYO_RS01115"
                     /old_locus_tag="pgaptmp_000223"
     CDS             complement(97635..98258)
                     /gene="tesA"
                     /locus_tag="ACHKYO_RS01115"
                     /old_locus_tag="pgaptmp_000223"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HGU4427126.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            98229..98915
                     /gene="ybbA"
                     /locus_tag="ACHKYO_RS01120"
                     /old_locus_tag="pgaptmp_000224"
     CDS             98229..98915
                     /gene="ybbA"
                     /locus_tag="ACHKYO_RS01120"
                     /old_locus_tag="pgaptmp_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HGU4427127.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            98912..>101222
                     /gene="ybbP"
                     /locus_tag="ACHKYO_RS01125"
                     /old_locus_tag="pgaptmp_000225"
     CDS             98912..>101222
                     /gene="ybbP"
                     /locus_tag="ACHKYO_RS01125"
                     /old_locus_tag="pgaptmp_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HGU4427128.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLN
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKQIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLW"
BASE COUNT        24742 a        25649 c        26915 g        23916 t
ORIGIN      
        1 acgttgctgg gatcaccgaa gcggcgaaag aaacccagcc ggaagcgtat attgtcggcg
       61 aacattttgg cgatgcacgg caatggttac aggccgatgt ggaagatgcc gccatgaact
      121 atcgtggctt cacattcccg ttgtggggat ttcttgccaa taccgatatc tcttacgatc
      181 cgcagcaaat tgatgcccaa acctgtatgg cctggatgga taattaccgc gcagggcttt
      241 ctcatcaaca acaattacgt atgtttaatc agctcgacag ccacgatact gcgcgattta
      301 aaacgctgct cggtcgggat attgcgcgcc tgccgctggc ggtggtctgg ctgttcacct
      361 ggcctggtgt accgtgcatt tattacggtg atgaagtagg actggatggc aaaaacgatc
      421 cgttttgccg taaaccgttc ccctggcagg tggaaaagca ggatacggcg ttattcgcgc
      481 tgtaccagcg aatgattgcg ctgcgtaaga aaagtcaggc gctgcgtcgt ggcggctgtc
      541 aggtgctgta tgcggaagat aacgtagtgg tatttgtccg cgtgctgaat cagcaacgtg
      601 tactggtggc aatcaaccgt ggcgaggcct gtgaagtggt gctccccgcg tcaccgtttc
      661 ttaatgtcgc gcaatggcaa cgcaaagaag gccatgggca actgacaaac gggattctgg
      721 ctttgcctgc catttcggct acggtatgga tgaactaacg ttttataacg ccttgtgtga
      781 cgcctgcgcc atcatccgcg gataaaactg ccagaagcgg gtttcgaggg cgtcataatg
      841 agcgtctaaa tcgtaccagg agtcacgcag ggcatccaga cgtgggcggc ggcttgccat
      901 gccgtttaac acgttctgga tgaaatccat atcgcgatag cgcaccagcc actgctctga
      961 ccacaagtaa ttgttcagat tgataaaacg tggcggtgag tccggcaaaa tcgtcatcac
     1021 ttgctcgcgg gcataacaga caaattcctg tagcggaaag tccggcgaca gctgcgacca
     1081 gtggcgggaa agaaagtgat cccacatgac atccagcgta ataggcgcaa cgcggcgagt
     1141 ttcactacga aaccattccc gtgcttcgcg gacttccggc agattgtcgg ttaatacgtc
     1201 gatacgtcga tgcatatgaa tgccagccac gacgtcgggc ggaaaacttt cttcaggatt
     1261 tccgcgtacg aaatcagcca gtaaattgcc ggaaagcgag ctttccgcga gatgggctaa
     1321 atgcaggtga gctaaaaaat tcatcgatta tattctatcc aaaagggggt aaaggttgca
     1381 gggagagcgc cccggcacta gactacccgc ctcttatttt agtctgagtc agtgtcatgc
     1441 gcgttaccga tttctccttt gaattgcccg aatccctgat tgcccactat cccatgcctg
     1501 aacgcagtag ctgtcgttta ctgtcgctgg acgggccgac gggcgcgctg acgcacggta
     1561 ctttcaccga tttacttgat aagctcaacc ccggcgatct tctggttttt aataataccc
     1621 gcgtgatccc ggcgcgcctg tttgggcgta aagccagcgg cggcaagatt gaagtgctgg
     1681 ttgagcggat gctcgacgac aaacgcattc ttgcgcatat tcgcgcctcg aaagcgccaa
     1741 aacctggcgc agaactgctg ctgggcgatg acgaaagtat taacgcaaca atgaccgcgc
     1801 gccacggcgc actgtttgaa gtcgaattca atgatgatcg ttcggtactg gatatcctca
     1861 acagtatcgg ccatatgccg ctgccgccgt atatcgaccg tccggacgaa gacgctgacc
     1921 gcgaacttta tcaaaccgtt tatagcgaaa aaccgggcgc ggttgcagcc ccgaccgctg
     1981 gtctgcattt tgacgagcct ttgctggaaa aattgcgcgc caaaggcgtg gagatggcgt
     2041 ttgtgacgtt gcacgttggt gcgggcacct tccagccggt gcgcgtcgac accattgaag
     2101 atcacatcat gcactcggaa tacgctgaag taccgcagga tgtggtagac gcggtactgg
     2161 cggcgaaagc gcgcggtaac cgggtgattg cggttggcac cacttcagta cgttcgctgg
     2221 aaagcgcggc tcaggcagcg aaaaacgatc tcattgaacc gttcttcgac gatacccaaa
     2281 tctttatcta tccgggcttc cagtacaaag tggtcgatgc gctggtgacg aacttccact
     2341 tgccagagtc gacgctgatt atgctggttt cggcctttgc cggttatcaa cacaccatga
     2401 acgcctataa agcagcggta gaagagaaat atcgcttttt tagttacggt gatgcgatgt
     2461 ttatcacgta caatccgcag gcaattaatg agcgcgtcgg ggagtaattc cgcggcgctg
     2521 gtttaaaacg ttggactgtt tttctgacgt agtggagaaa aaatgaaatt tgaactggac
     2581 accaccgacg gtcgcgcacg ccgtggccgc ctggtctttg atcgtggcgt agtggaaacg
     2641 ccttgtttta tgcctgttgg cacctacggc accgtaaaag ggatgacgcc ggaagaagtt
     2701 gaagccactg gcgcgcaaat tatcctcggc aacaccttcc acctgtggct gcgcccgggc
     2761 caggaaatca tgaaactgca cggcgatctg cacgatttta tgcagtggaa ggggccgatc
     2821 ctcaccgact ccggcggttt ccaggtcttc agccttggcg atattcgtaa aatcaccgaa
     2881 cagggcgtgc acttccgtaa cccgatcaac ggcgatccga ttttcctcga tcctgaaaaa
     2941 tcaatggaga ttcagtacga tcttggttcg gatatcgtca tgatctttga tgagtgtacg
     3001 ccgtatcctg ctgactggga ttacgcaaaa cgctccatgg agatgtctct gcgttgggcg
     3061 aagcgtagcc gtgagcgttt tgacagtctc ggaaacaaaa atgcgctgtt tggtatcatt
     3121 cagggcagcg tttacgaaga tttacgtgat atttctgtta aaggtctggt agatatcggt
     3181 tttgatggct acgctgtcgg cggtctggct gtgggtgagc cgaaagcaga tatgcaccgc
     3241 attctggagc atgtatgccc gcaaattccg gcagacaaac cgcgttacct gatgggcgtt
     3301 ggtaaaccag aagacctggt tgaaggcgta cgtcgtggta tcgatatgtt tgactgcgta
     3361 atgccaaccc gcaacgcccg aaatggtcat ttgttcgtga ccgatggcgt ggtgaaaatc
     3421 cgcaatgcga agtataagag cgatactggc ccactcgatc ctgagtgtga ttgctacacc
     3481 tgtcgcaatt attcacgcgc ttacttgcat catcttgacc gttgcaacga aatattaggc
     3541 gcgcgactca acaccattca taaccttcgt tactaccagc gtttgatggc gggtttacgc
     3601 aaggctattg aagagggtaa attagagagc ttcgtaactg atttttacca gcgtcagggg
     3661 cgagaagtac cacctttgaa cgttgattaa tattaataat gagggaaatt taatgagctt
     3721 ttttatttct gatgcggtag cggcaacggg tgcaccggcg caaggtagct cgatgtcttt
     3781 gattttgatg ctggtggtat tcggtctgat tttctatttc atgatcctgc gtccacagca
     3841 gaagcgcacc aaagaacaca aaaagctgat ggactccatt gccaaaggtg atgaagttct
     3901 gacgaacggt ggcctggttg gccgcgtaac caaagtagcg gaaaacggct acattgctat
     3961 cgcgctgaat gacaccactg aagtagttat taaacgtgac ttcgtagctg ccgtcctgcc
     4021 gaaaggcacc atgaaggcgc tgtaattaaa atttttccct aagggaattg ccgtgttaaa
     4081 ccgttatcct ttgtggaagt acgtcatgct gatcgtggtg attgtcatcg gtctgctgta
     4141 tgcgcttccc aacctgtttg gtgaggatcc ggctgttcag atcactggtg cgcgcggagt
     4201 cgccgccagt gagcaaacgc tgatccaggt ccagaaaacg ttacaagaag aaaaaataac
     4261 tgctaagtct gtggcactgg aagagggcgc tattcttgcg cgcttcgact ccactgacac
     4321 ccagctgcgc gctcgtgaag cattaatggg cgttatgggt gacaaatacg tcgtggcgct
     4381 taaccttgcc ccggcaacgc cgcgctggct ggcagctatt cacgctgagc cgatgaagct
     4441 cggccttgac ctgcgtggcg gcgttcactt cctgatggaa gtggatatgg acaccgcgct
     4501 tggcaaactc caggaacaaa atatcgatag cctgcgcagc gatctgcgtg aaaagggcat
     4561 cccgtatacc accgttcgta aagaaaacaa ctacggcctg agcattactt tccgcgatgc
     4621 taaagctcgt gatgaagcca ttgcgtatct gagcaagcgc catccggacc tggtgattag
     4681 cagccagggc agcaaccagc tgcgtgcggt aatgagcgat gctcgtctga gtgaagcgcg
     4741 tgaatatgcg gtgcagcaga acattaatat cctgcgtaac cgtgtaaacc aacttggcgt
     4801 ggcggagccg gtggttcagc gtcagggtgc tgaccgtatc gttgttgaac tgccaggtat
     4861 tcaggacact gcgcgtgcga aagagattct gggtgcgacg gcaacgctgg aattccgtct
     4921 ggtaaacacc aacgttgacc aggccgctgc ggcatccggt cgcgtaccgg gcgactctga
     4981 agtgaaacag acccgcgaag gtcagccagt tgtgctgtac aaacgcgtaa ttctgaccgg
     5041 tgaccatatc accgactcca cttccagcca ggacgaatac aaccaaccgc aggttaacat
     5101 ctcgctcgat agcgctggtg gtaacatcat gtctaacttc actaaggaca acatcggcaa
     5161 accgatggca accctgtttg tggagtacaa agacagcggt aagaaagatg caaatggtcg
     5221 tgcggttctg gtgaaacagg aagaggtgat taacatcgcc aacatccagt ctcgtctggg
     5281 taacagcttc cgtatcaccg gcatcaacaa cccgaacgaa gcccgtcagc tgtcactgct
     5341 gctgcgtgcc ggtgcgttga tcgcgccgat tcagattgtt gaagaacgta ccattggccc
     5401 aaccctgggt atgcagaaca ttgaacaggg gctggaggct tgccttgccg gtctgctggt
     5461 ttctattctg ttcatgatca tcttctataa gaagtttggt ctgattgcga ccagtgctct
     5521 gattgccaac ttgatcttaa tcgtcggcat tatgtcgctg ttgccaggcg caacgctgag
     5581 tatgccaggt atcgcgggta tcgtcttaac ccttgcggtg gcggtcgatg cgaacgtact
     5641 gatcaacgaa cgtattaaag aagagttgag caacggacgt actgttcaac aggcaattga
     5701 tgaaggttat cgtggcgcat tcagttctat cttcgatgcg aacatcacca cgctgattaa
     5761 agtcatcatc ctgtacgcag tgggtaccgg ggcaattaaa gggttcgcga ttactaccgg
     5821 tatcggtgtg gcgacgtcga tgtttaccgc gattgtcggt acgcgtgcca tcgtaaacct
     5881 gctatatggc ggcaagcgcg tcaagaagct gtcaatctga ggagtgcgat gtggcacagg
     5941 aatatactgt tgaacaacta aaccacggcc gtaaagtcta tgactttatg cgctgggact
     6001 actgggcttt cggcatctct ggtctgctgt taatcgctgc tatcgttatt atgggcgtgc
     6061 gcggctttaa ctgggggctg gattttaccg gtggtacggt tatcgaaatt acgctcgaaa
     6121 aaccggctga aattgacgta atgcgtgatg cattgcaaaa agccggtttt gaagagccaa
     6181 tgctgcaaaa ctttggtagc agccacgaca tcatggtccg tatgccgcct gctgaaggcg
     6241 aaaccggcgg tcaggtgctg ggcagccagg ttctgaaggt gattaacgaa tccaccaatc
     6301 agaatgcagc agtgaagcgt attgagttcg tcggtccgag cgtgggggca gaccttgcgc
     6361 aaaccggtgc gatggcgttg atggcagcgc tgctgtctat cctcgtgtac gtaggtttcc
     6421 gctttgagtg gcgactggcg gcaggggtgg ttattgcgct ggcgcacgac gttatcatta
     6481 cgctgggtat tttgtcgtta ttccatatcg agattgacct gaccattgtg gcatcgttga
     6541 tgtcggttat cggttactcg cttaacgaca gtatcgtggt atcggaccgt attcgtgaaa
     6601 acttccgcaa gatccgtcgc ggtacgcctt acgaaatctt taacgtgtcc ttgacccaga
     6661 cgctgcaccg taccttgatc acatccggta ctaccttgat ggttatcctg atgctgtacc
     6721 tcttcggtgg tccggtactg gaaggcttct cgctgaccat gcttatcggt gtttccatcg
     6781 gtactgcatc ttccatctat gtggcatctg cgttggctct gaaactgggt atgaagcgcg
     6841 aacacatgtt gcagcagaaa gtggaaaaag aaggggcgga tcagccgtca attctgcctt
     6901 aatcaagttc ccgttgatgt tgaaaatccc ggtcagaaga tcgggatttt ttttgatgta
     6961 tggagattgc gaaaatcccc gcatcttggg aaactgcgcg taaccctaca tttcatccag
     7021 gtaacttttc atggctatca tcccaaaaaa ctatgcgcgg ttagaaagcg gctatcgcga
     7081 aaaagcatta aaaatctatc cgtgggtctg cggtcgctgt tcccgcgagt ttgtctattc
     7141 caacctgcgt gaacttactg ttcaccacat tgatcacgac cataccaata acccggaaga
     7201 tggcagtaac tgggaattgc tttgtctcta ttgccacgat catgagcatt cgaaatatac
     7261 cgaagcggat cagtatggta cgaccgttat cgcaggggaa gatgcgcaga aagatgtcgg
     7321 tgaagcgaag tacaacccat tcgctgacct gaaagcgatg atgaacaaga agaagtgatt
     7381 aaaacgtaaa attgcctgat gcgctacgct tatcaggact acgttatttc agcaatatat
     7441 tgaatttacg tgcttttgtg ggtcggacaa agcgtttacg ccgcatccgg catgaacaaa
     7501 gcgcacgttg ttaacaatct gaaatgccgg gaataaatcc cggcattttc ataatcagaa
     7561 gttgtaacct acaaccaggt aaccacccca gccggtagag cgaacgttga agttgccgtt
     7621 gccgaagttc agttctgcat cgtcgttcca ctgaccaccg tcgtgccagt aacgagctac
     7681 gacagagtag tgccagtgat cgtagttcag agccagaata tggctggaag cgatagagtt
     7741 attagtacgg gtcttaatac cgttgattgc gttaccgctg tcatccccta aatcggaacc
     7801 ccagtcgaag ttggtgaagc cgatgtagct cagctgaccg ccccacagat cggtaatcgg
     7861 cacaaagtat ttaattttga aacggtaacc gtcccactcg ttttcgttcg ctgcgccata
     7921 gttctgccac tggtatttcg catagacgtt catggacagg ctcatcggca gaccagtatc
     7981 gatgtcggta cccagaccca tataccaggt gctctggcga ccatctttat tacgacccat
     8041 gtcgtaaatg tagttgttcg cgaagtacca ctctttgaac ggaccgaagc taaggtcagt
     8101 attggtcagc ttgtcgatgg agaaacgtgg ttcgatttcc ataaacagcg gagagccgtg
     8161 gttccagata cctttggcat cggagttacc gccgaagaat accggcgcat ccgcataacc
     8221 atagaagtcg aaccagtctt ttttagcgaa tgcttcgtac tcaaggtagg tatcgttgcg
     8281 gatctgcggt ccaaaacggg tgtgatagct tccgacaacg ttaacgctct ggtgccacca
     8341 gtcggaaaga tactgcggtt tgtcgttttc agctgcgttg acagtaaaag acgaagagag
     8401 cgccagtacc gcaccggctg ccagtaatgt ttttttcata tgtatgccac tgtttgaaaa
     8461 tcccttgcgg gagtgaaaaa ggcgcaaatt gcgtttctaa atatttcgta tacagaggag
     8521 cctattataa aaatcattgc tcacaaaaat atgtttcgtt tcacagttct atcatttacg
     8581 taatcgattg cgttcacgtt tacacacatt cgggcaggga ttgtactgac tttcaccctt
     8641 gttgcaaact tttactatca atctgggtga tgtaagcgga gtaacaaaat gacggggaaa
     8701 gatgttgcgg gacgcacaaa atcgccccga aatacgttta ctgcataaca ggttgaatat
     8761 cgtggatacg tacgaagtct aactgttcta cagaaatacc ctttaactgt aacggaatat
     8821 caacatcgct gggggcgagg acgctggcag gggcattaat caattgattc tgcacattga
     8881 tttcctgaaa gttgtctatt gttccctgaa tttgcccata ttcaacagtt ccgctgaatg
     8941 cgggcaaggg atcattcgac tcgccctgaa tacgtagcgt cagggtggtg ccatctgcat
     9001 caggcgtaat attcaccagc gacatacgta aagtaccgat ctggctttcc agtcttgccg
     9061 gtgttttcgc tcctggcaga aggtaaaccc cactgctgga ttttgcattc agcctgtttt
     9121 gctgagtgat tttgaccgtt tctttattga gttgcgtcat ctcctggttc agcgtgctga
     9181 cgctgtgttt catctgtcgc acttcgctct gctgcacaca ggcgctaagg ctgaacaggc
     9241 ttcccagcag gagcagtcga aaaacgttag tattcatagg tcgtcccttt ctcgtcgcaa
     9301 ttagcctaat ggtagagggt aaaagcagcg aaagcatcga tcctttatct caaaagcgtt
     9361 gcgcctttgt tgtatcgtca gttcagggta aaatagattt ctgttaacca cctggtcagg
     9421 acgccgtatg cattgcccat tctgtttcgc cgtggacact aaggtaattg actctcgtct
     9481 cgtgggcgag ggttcatccg tacgccgccg tcggcagtgt ctggtgtgta atgaacgttt
     9541 caccaccttt gaagtggcgg agctggttat gccgcgtgtt gtaaaaagca acgacgtgcg
     9601 tgaaccgttt aatgaagaga aattgcgtag cggaatgctg cgggcgctgg aaaaacgtcc
     9661 ggtgagttcc gatgacgtcg aaatggcaat caatcatatt aaatcgcagc tgcgcgccac
     9721 cggtgagcgc gaagtgccga gcaagatgat tggcaatctg gtgatggagc aattgaaaaa
     9781 gctcgataaa gtcgcctata tccgttttgc ctctgtctac cgcagtttcg aagatatcaa
     9841 agaatttggc gaagagatcg cgcgcctgga ggactaagcc gtgcaggacg agtattacat
     9901 ggcgcgggcg ctaaagctgg cgcaacgagg acgttttacc acccacccca acccgaatgt
     9961 cgggtgcgtc attgtcaaag atggcgaaat tgtcggtgaa ggttaccacc aacgagcggg
    10021 tgaaccacat gccgaagtac acgcgttgcg tatggcgggt gaaaaagcca aaggcgcaac
    10081 ggcgtatgtg acactcgaac cctgtagcca tcatggtcgt acgccaccgt gctgtgacgc
    10141 actcatcgcc gctggcgtag cgcgcgtggt tgcctcgatg caagatccta acccgcaggt
    10201 cgctgggcgt ggactttacc gtctgcaaca ggctggcatt gacgtcagcc acgggctgat
    10261 gatgagtgaa gccgagcaat tgaataaagg ctttctcaag cggatgcgca ccggctttcc
    10321 ttatattcag ttaaaacttg gcgcatcgct tgatggccgc acggcgatgg cgagcggcga
    10381 aagccagtgg atcacttcgc ctcaggcgcg gcgcgacgta caacgacagc gcgcacaaag
    10441 tcatgccatt ttaaccagca gcgccacggt gctggcggat gatccagcct taacagtgcg
    10501 ttggtctgaa ctggatgaac aaactcaggc gctctacccg caacaaaatc tccgccagcc
    10561 agtacgtatt gtgattgata gccaaaatcg cgtgacgccg gaacatcgca ttgtgcagca
    10621 gcccggcgaa acctggttcg cgcgtacgca ggaagattct agtgagtggc cggaaacggt
    10681 gcgtaccttg ctgattccag agcataaagg tcatctggat ctggttgtac tgatgatgca
    10741 actgggtaaa cagcaaatta acagcatctg ggtggaagcg gggccaacgc tcgctggcgc
    10801 attgctgcag gtgggtttag tcgatgagct gattgtctat atcgcaccta aactattagg
    10861 cagcgacgcc cgcggattat gcacgctgcc agggcttgag aaattagccg acgcccccca
    10921 atttaaattc aaagagatac gtcatgtagg cccggatgtt tgcctgcatt tagtgggtgc
    10981 atgatctccc ggctcgaaag ggaagcagcg cacgaaatat tatgctaaaa tccgcccccc
    11041 tgcggggcca tactcgaacc cgaaggaaga aaatgaacat tattgaagct aacgttgcta
    11101 ccccggacgc tcgcgtcgcc atcaccatcg cgcgtttcaa caactttatc aatgacagcc
    11161 tgctggaagg tgcaattgac gcactgaaac gtatcggtca ggtaaaagat gaaaacatta
    11221 ccgttgtttg ggtgcctggt gcctatgagc tgccgctggc ggcgggtgca ctggctaaaa
    11281 ccggtaaata cgacgcggtg attgcgctgg gtacggttat tcgtggtggc actgcccact
    11341 ttgaatatgt cgctggtggt gcaagcaacg gcctggcgca tgttgcccag gacagcgaaa
    11401 ttccggttgc ttttggggtt ctgaccactg aaagcattga acaagcgatc gaacgtgctg
    11461 gcaccaaagc tggcaacaaa ggtgcagaag ctgcactgac cgcgcttgaa atgattaatg
    11521 tattgaaagc catcaaggcc tgaaattagt aaggggaaat ccgtgaaacc tgctgctcgt
    11581 cgccgcgctc gtgagtgtgc cgtccaggcg ctctactcct ggcagttgtc ccagaacgac
    11641 atcgctgatg ttgaatacca gttcctggct gaacaggatg taaaagacgt tgacgtcctg
    11701 tacttccgtg agctgctggc cggggtggcg actaacaccg catacctcga cggactgatg
    11761 aagccatacc tgtcccgcct gctggaagaa ctgggacagg tagaaaaagc agtactgcgc
    11821 attgcgctgt acgagctgtc taaacgtagc gatgtgccat acaaagtggc cattaacgaa
    11881 gcgatcgaac tggcgaaatc gttcggcgca gaagacagcc ataagttcgt caacggcgta
    11941 ctcgataaag cagcacctgt gattcgccct aacaaaaagt gatatccagg ccggtagatt
    12001 cacggaagac cgttccgcga tcgccggcct tttctttttt acctgctgag gcataacgta
    12061 tggcatgtgg cgagttctcc ctgattgccc gttattttga ccgtgtaaga agttctcgtc
    12121 ttgatgtcga actgggcatc ggcgacgatt gcgctcttct caatatcccc gagaaacaga
    12181 ccctggcgat cagcactgat acgctggtgg cgggcaacca cttcctccat gatatcgatc
    12241 ctgctgatct ggcgtataaa gcactggcgg tgaacctaag cgatctggca gcgatggggg
    12301 ccgatccggc ctggctgacg ctggcattaa ccttaccgga cgtagacgaa gcgtggcttg
    12361 agtccttcag cgacagtttg tttgatcttc tcaattatta cgatatgcaa ctcattggcg
    12421 gcgataccac gcgtgggcca ttatcaatga cgttgggtat ccacggcttt gttccgatgg
    12481 gacgagcctt aacgcgctct ggagcgaaac cgggtgactg gatctatgtg accggtacac
    12541 cgggcgatag cgccgccggg ctggcgattt tgcaaaaccg tttgcaggtt gccgatgcta
    12601 aagatgcgga ctacttgatc aaacgtcatc tccgtccatc gccgcgtatt ttacagggac
    12661 aggcactgcg cgatctggca aattcagcta tcgatctctc tgacggtctg atttccgatc
    12721 tcgggcatat cgtgaaagcc agcgactgcg gcgcacgtat tgacctggca ttgctgccgt
    12781 tttctgatgc gctttctcgc catgttgaac cggaacaggc gctgcgctgg gcgctctctg
    12841 gcggtgaaga ttacgagttg tgtttcactg tgccggaact gaaccgtggc gcgctggatg
    12901 tggctctcgg acacctgggc gtaccgttta cctgtatcgg gcaaatgacc gccgatatcg
    12961 aagggctttg ttttattcgt gacggcgaac ctgtcacgtt tgactggaaa ggatatgacc
    13021 attttgccac gccataaaga tgtcgcgaaa agtcgcctga agatgagtaa tccgtggcat
    13081 ctgcttgctg tcggattcgg aagtggatta agcccaatcg ttcctggcac gatgggatcg
    13141 ctggcagcga ttccgttctg gtatctgatg acctttttac cctggcagct ctactcgctg
    13201 gtggtgatgt tggggatctg tatcggcgtc tatctttgtc atcaaacggc gaaagatatg
    13261 ggcgtgcacg atcacggcag cattgtctgg gacgaattta ttggtatgtg gatcacgctc
    13321 atggcgctgc cgaccaatga ctggcagtgg gttgccgccg ggtttgtgat tttccgtatt
    13381 ctggatatgt ggaagccgtg gccgatccgc tggtttgatc gcaatgtaca tggcggcatg
    13441 gggatcatga tcgacgatat tgtcgccggg gtgatttccg caggcatcct gtactttatc
    13501 ggtcatcact ggccgctggg tattctgtcg tagttgtgca ccgatgcctg atgtgacgct
    13561 tgtcacgtct catcaggcct ggactcttat ttaaatccta cgaccgcatg cggtttatac
    13621 ggcgtttcca gttcggcaat ctgttctggc ttcaaagtga tatccaccgc gttcagcagc
    13681 tcatcaagct gttcttcccg cgatgtaccg ataatcggtg cggcaatgcc cggtttactc
    13741 aacaaccagg ccagcgcaac ttgtgctcgt gttgcaccga gttcttcact gacgcccgtt
    13801 aaccgctctg cgatctgcgc gtcattttca tcgctttctt tatagagatt tctccccacc
    13861 tcatcagaca ccagtcgtgc ggtagtttct ccccacggac gcgtcagtcg cccccgcgcc
    13921 agcgggctcc acggaatcac cgccacgccc tcctgataac acagaggcag catctcgcgc
    13981 tcttcttcgc gataaatcag attgtagtga tcctgcatac tgataaactg cgcccagccg
    14041 tgctgttttt gtagcttcag ggcctgagca aactgcgaag cgtgcatgga tgacgcgccg
    14101 atataacgcg ctttcccggc ttttaccacg tcgttcaggg cttccagcgt ctcttcgatc
    14161 ggcgtgttgt aatcccagcg atgaatttgc aggatatcga cataatccat gccgagacgg
    14221 cgcaggctgt cgtcgataga gcgcaaaatt tgtgcacggg ataatccttc cggtaaatca
    14281 ccaacgcgat ggaacacttt cgtcgcaacg accacgtctt cacgacgggc gaaatcccgc
    14341 agtgcgcgac caacgatctc ttcgctgctg ccatcggaat agctattggc ggtatcaaag
    14401 aaatttatgc cgccttccag cgcgcgttta atgatgggac ggctactctc ttccggcagt
    14461 gtccatgcgt gattaccacg atctggctcg ccaaaggtca tacagccgag gcaaagtcgg
    14521 gaaacgcgaa ggtcggtttt tcctaagggg ttgtattgca tgctgccact cctgccatac
    14581 tcgtcatact tcaagttgca tgtgctgcgg ctgcattcgt tcaccccagt cacttactta
    14641 tgtaagctcc tggggcttca ctcgtttgcc gccttcctgc aactcgaatt atttagagtc
    14701 tatgaataat ttgttaagca tagcaggagt ggagtaggga ttatgccagc caggccttga
    14761 ttttggcttc cataccggcg gcatcgaggc cgagttcggc gcgcatttct tcctgagttc
    14821 cttgcggaat aaagaagtca ggcaggccaa tgttcagcac gggtactggt ttacgatggg
    14881 ccattagcac ttcgttcacg ccgctgcctg cgccgcccat aatggcgttt tcttctacgg
    14941 tgaccagcgc ttcatggctg gcggccattt ccagaattaa cgcttcatca agcggtttca
    15001 caaaacgcat atcgaccagc gtggcgttca gcgattcggc gactttcgcc gcttctggca
    15061 tcagcgtacc aaagttaagg atcgccagtt tctcgccacg acgcttcaca atgcctttgc
    15121 caattggtag tttttccagc ggcgtcagtt ccacgccgac cgcgttgcca cgcgggtagc
    15181 gcaccgctga cgggccatcg ttatagtgat agccggtata gagcatctgg cgacattcgt
    15241 tttcatcgct cggggtcata atgaccattt ccggtatgca gcgcaggtaa gagaggtcaa
    15301 aagcgccctg atgggtttga ccgtcagcac caacaatgcc cgcgcggtcg atggcgaaca
    15361 ggaccgggag cttttgaatc gccacgtcat gcagcacctg atcataggcg cgttgcagga
    15421 aagtggagta aatcgcgaca atgggtttgt acccaccaat cgccagaccg gcggcaaagg
    15481 tcaccgcgtg ttgctcggcg attgccacgt cgaagtaacg atccgggaat ttacgtgaaa
    15541 actcgaccat gccggaacct tcacgcatcg ccggagtaat cgccatcagc ttgttgtctt
    15601 tcgctgccgt ttcgcacaac cagtcgccaa agatttttga atagctcggc aaaccgccgc
    15661 tacttttcgg caaacaaccg ctggagggat caaatttagg cacggcgtgg aaagtgatgg
    15721 ggtctttttc tgccggttca taaccacgac cttttttggt catgatatgc aggaactgcg
    15781 ggcctttcag gtcgcgcatg ttcttcagcg tggtgataag ccccagcaca tcgtgaccgt
    15841 caaccgggcc gatgtagtta aagcccagct cttcaaacaa cgtgccaggc actaccatgc
    15901 ctttaatatg ttcttcggta cgtttgagca gctctttaat tggcggaacg ccagagaaaa
    15961 cttttttccc gccttcgcgc agcgaagagt aaagcttacc ggaaagcagc tgtgccagat
    16021 ggttattgag cgcgccgaca ttttcggaaa tcgacatttc attgtcgttg aggaccacca
    16081 gcatatcagg acggatatcg cccgcgtgat tcatcgcttc aaacgccatg ccagcggtaa
    16141 tcgcgccatc gccaatgaca cagacggtgc ggcgattttt gccttctttc tcggcagcaa
    16201 ccgcaatacc aattccggca ctgatggagg ttgatgaatg cccgacgctt aatacgtcat
    16261 attcgctttc gccgcgccac gggaacgggt gcaggccgcc tttctgacgg atggtgccga
    16321 ttttgtcgcg gcgtccggtc aaaattttat gcggataagc ctgatgcccc acatcccaaa
    16381 tcaattggtc aaacggggtg ttgtagacat agtgcagcgc cacggtcagt tcgaccgtgc
    16441 ccagcccgga ggcgaagtgc ccgctggaac ggctcacgct gtcgagtaaa tagcggcgca
    16501 gttcgtcgca gagtttcggt aaactctctt tcggcaacag tcgtaactcc tgggtggagt
    16561 cgaccagtgc cagggtcggg tatttggcaa tatcaaaact catcaggggc ctattaatac
    16621 ttattgttta tttattacgc tggatgatgt agtccgctag cgcttccagt gccgaggtat
    16681 cgagtgattg ttcagccagt tgtttcagcg actggcgggc atcgtcgatc agatcccggg
    16741 ctttcttccg ggcttgctca agacccagaa gtgcagggta ggtactttta ccaagttgct
    16801 ggtcggcacc ctggcgtttt cccaacgttg cagtatctcc taccacatcc aggatgtcat
    16861 cctgaacctg gaaggcaagg ccgatgctct ctgcgtactt gtcgagtact ggcagagcac
    16921 gacgcccttt atctccggcg cttaatgcac caaggcgaac ggcggcgcga atcaatgcgc
    16981 cggttttatg acgatgaata cgctcaagcg cgtccagagg tacgtgtttg ccttccgcgt
    17041 ctaaatctaa tgcctgacca ccgcacattc cggcaatacc gctggcgctc gccagttcag
    17101 aaatcatcga aattctgtcg cgatccgaca cttccggcat atcagcatcg cttagaatcg
    17161 agaacgccag cgtttgtaaa gcgtcgccag cgagaatcgc gtttgcttcg ccaaacttca
    17221 catggcaggt cggcaaaccg cgacgcagat cgtcatcatc catcgccggt aaatcatcat
    17281 gaattaatga gtaagcgtgg atacactcta cggcggcagc gggtgcgtcc agcgtgtttg
    17341 tgctaacgcc gaacatatga ccggtggcat aaaccagaaa aggtcgcagg cgcttaccac
    17401 ctaataatgc gccatactgc atggtttcga ccacgggagt gttctgaaag ggcagtgggg
    17461 cgataaaacg gctcagcgcc tggttggcct gcttaacgca ggcttcgagt tgctgcggaa
    17521 agtccattac tcattgtccg gtgtaaaagg ggttagagag gcgtcttcat tgtcagacag
    17581 cagaatttgt acgcgctgtt cggcttgttg taatttggcc tgcccctgac gtgccagctg
    17641 cacgccgcgt tcgaactcgt tcagcgcctc ttccagcggc aggtcgccac tttccagacg
    17701 ggttacaatc tgttccagct cgctcagcgc cttttcaaag ctggcgggag cctcattttt
    17761 cttcggcata atgaatgtct gactctcaat atttttcgcc ccgtcatggt aacggactca
    17821 gggcaaatag caaataacgc gcaatggtaa ggtgatgtgc gcagcaaagc gatgttagtg
    17881 gtatacttcc gcgcctggat gcagccgcag gtgtgggctg ctgtattttt ccctatacaa
    17941 gtcgcttaag gcttgccaac gaaccattgc cgccatgaag tttatcatta aattgttccc
    18001 ggaaatcacc atcaaaagcc aatctgtgcg cttgcgcttt ataaaaatcc ttaccgggaa
    18061 cattcgtaac gttttaaagc actatgatga gacgctcgct gtcgtccgcc actgggataa
    18121 catcgaagtt cgcgcaaaag atgaaaacca gcgtctggct attcgcgacg ccctgacccg
    18181 tattccgggt atccaccata ttctcgaagt cgaagacgtg ccgtttaccg acatgcacga
    18241 tatttttgag aaagcgttgg ttcagtatcg cgatcagctg gaaggcaaaa ccttctgcgt
    18301 acgcgtgaag cgccgtggca aacatgattt tagctcgatt gatgtggaac gttacgtcgg
    18361 cggcggttta aatcagcata ttgagtccgc gcgcgtgaag ctgaccaatc cggaagtgac
    18421 tgtccatctg gaagtggaag acgatcgtct cctgctgatt aaaggccgct acgaaggtat
    18481 tggcggtttc ccgatcggca cccaggaaga tgtgctgtcg ctcatttccg gtggtttcga
    18541 ctccggtgtt tccagttata tgttgatgcg tcgcggctgc cgcgtgcatt actgtttctt
    18601 taaccttggc ggcgcggcgc atgaaattgg cgtgcgtcag gtggcgcatt atctgtggaa
    18661 ccgttttggc agctcccacc gcgtgcgttt tgtcgctatt aatttcgaac cggtcgtcgg
    18721 ggaaattctc gagaaaatcg acgacggtca gatgggcgtt atcctcaaac gtatgatggt
    18781 gcgtgccgcg tctaaagtgg ctgaacgtta cggcgtacag gcgctggtca ccggcgaagc
    18841 gctcggccag gtgtccagtc agacgctgac caacctgcgc ctgattgata acgtctccga
    18901 tacgctgatc ctgcgtccgc tgatttctta cgacaaagag cacatcatca acctggctcg
    18961 ccagattggc accgaagact ttgctcgcac gatgccggaa tattgcggtg ttatctccaa
    19021 aagcccgacg gtgaaagcgg ttaagtcgaa gattgaagcg gaagaagaga aattcgactt
    19081 cagcattctc gataaagtgg ttgaagaagc gaataacgtt gatatccgcg aaatcgccca
    19141 gcagaccgag caggaagtgg tggaagtgga aaccgtcaat ggcttcggcc cgaacgacgt
    19201 gatcctcgat atccgttcta tcgatgaaca ggaagataag ccactgaaag tcgaagggat
    19261 tgatgtagtt tctctgccgt tctataaact gagcaccaag tttggcgatc tcgaccagaa
    19321 cagaacctgg ctactgtggt gtgagcgcgg ggtgatgagc cgtctgcagg cgctctatct
    19381 gcgcgagcag ggctttaaca atgtgaaggt atatcgcccg taatttgtgg ttgtaacgtc
    19441 gcatctggtc agatgcgacg cttaccgcat ccgacactac tcgtaataat tataaatccc
    19501 tgccgccatc accagttgtg atgccacttc atgggctttc tcgcgcccaa ccagcaggtc
    19561 gataattttc agaccaaagt cgatagctgt ccccggcccc tggctggtca gcaattttac
    19621 ccgtgcatcc cagacgacgc gcttgtcctg ccattgttca gcgggaattt tgtctttcag
    19681 cgtcgggaag ccggtcatat taccaatcgg gaagatatcg tgcggcacca gcacggtggc
    19741 tggcgcggcg caaatagccg cgacgatacg cccggaacgg tggaactgtt taacggtttc
    19801 aaccagcagg gtactatcgc gaaaacactc tgcgccttta atgccaccag gcagtacgat
    19861 cacgtcatat tcgccatcag ccacctcgac cagcggcgca tccgccagca gcttcacgcc
    19921 gcgcgagcag gtaatggcca ggttgccatc gctggcgacg ctggcagtgg tgactttgat
    19981 accgccgcga accagcagat cgatagtggt gacggcttca gtctcttcac taccaggggc
    20041 gaggcaaacc agtgccgatg cgctcatatt cactctcctt tctttttacc atttcaaaca
    20101 ggcgggtgtt ttccggtacg gcaatcccat gcgcgcgggc gcggcgtaag agaaaaccat
    20161 tgatatagtc gatttcggtg tggcgcagcg cgcggatatc ctgcaacatc gacgagatat
    20221 tttccgctgt ggcatcaatc acctgcatca cgtaatcacg caaatcttct gctgaagtat
    20281 gatgcccttc gcgctcgatc actgccgcga cttcttcgca tatctgcata atttcttgcg
    20341 gatgatgacg taattcacca ttcgggcaat tccagatggc ggtcagtgga ttaatcacac
    20401 agttgacggc cagcttgcgc cacagctcgg cgcgaatatt gttatgccag gcaacgtcag
    20461 gcaacacggt ttgcaaaata tccgccagat aactgtaatc gccatcctgt tgccgtgccg
    20521 gaccaatatg cgtgattccg tttgccacat gaataatgac attgccgtcg cggcgggcgg
    20581 catgggtggt ggtgcccatc agtaatggct gctgaatgtt ttgcaactct tcgatggtgc
    20641 ccatgccgtt gtgaattaac agtattggcg tggttacagg cagtgtggac gcgaggcttt
    20701 tgacggcatc gtaaacctgc catgctttca gcgtcaccag gagcagatcg ctggtggcaa
    20761 gaaaatcggg atcgttggcg gtcagcgatt cgttaaatat cgaaccatct gtctcaacca
    20821 gattcacgct acaataaggt tgcggtacgc gcagccagcc ctgaacttca tgaccctgtt
    20881 tgcaaagtgc tgtaagccat aattgcccta aggcaccgca tcccaatacg gtaattttca
    20941 ttgttcctcc tcacccgcaa ccactccggg tgttcaataa ggctatccct taattgtgca
    21001 tgctgttgcg actatgcaca attaagggat acgtcctggt gcaggactgt cggttattta
    21061 actttgcggg tattatgctt cgcatcaaaa atgaagggag aggaaaagat gccatctttc
    21121 gatattgtct ctgaagttga tcttcaggaa gcacgtaacg cggtcgataa cgcgagccgc
    21181 gaagtggagt cccgttttga cttccgtaac gttgaagcct catttgagct gaacgacgcc
    21241 agcaaaacca tcaaagtgtt gagcgagtcc gatttccagg tcaatcagtt gctggatatt
    21301 ctgcgtgcca agctgctgaa gcgcggcatt gaaggcagtt cgctggatgt accggagaat
    21361 atcgttcata gcggtaaaac ctggtttgtg gaagcgaaac tgaaacaggg cattgagagc
    21421 gcgacccaga agaaaatcgt caagatgatc aaagacagca aactgaaagt gcaggcgcaa
    21481 attcagggcg atgaaatccg cgtaacgggc aaatctcgtg atgatttgca ggctgtcatg
    21541 gcgatggtac gtggtggcga tctcggtcag ccgttccagt tcaaaaactt ccgcgattaa
    21601 tcgcgactat gtgcgttttg ttcatgtcag atgcggcatg aacgcctgat tcggcctaca
    21661 aaggtttgta attcagaaac tttgtaggcc tgataagcgt agcgcagcag gcaattcgcc
    21721 gcgtttttta tgcctgacga attgcctgtt ctatctcaaa gcgattcgtc actttgctgt
    21781 cgattttcac ataagctgaa tgttcttctt ctgcaatcaa cacttctttg atgccttcag
    21841 tttccagcaa acgcactttt aacgcttcgt ttgcggcaat gtccgccggg atttcaatgc
    21901 gcaaactgct gacatacggc ggttctttca tggtactggc gactgccagc cacactgcgg
    21961 ccagcattgc gccagcgaga aataccccct gaccgtcaaa catgccgtca atccagccgc
    22021 ccagcgaacc gccaatcgcc acgccaagaa actggctggt ggagtagaca cccatcgccg
    22081 tacctttgta acctgctggc gactctttac tgataagtga gggcagaagg gcttccatca
    22141 aattaaacgc cacaaagaaa agctgcacgc cgaccaccag ttgccagaac tgcgtttgtg
    22201 cgttccacaa cacaatttcc gcaaccacga tcaacccgac gcagaagaca aagacttgct
    22261 tcattttgcg cttaacttca gcgtagataa tgaaaggcac gaccgagcca aaggcgatta
    22321 acatcgtcgc cagatagacc ttccagtgtt cagccgccgg gaaccccgca tcagctagct
    22381 gtccgggcag ggcaacaaac gtagacatca gaagcatgtg cagacacata atgccaaagt
    22441 tgagttttag cagccgcggt tccgccagca ctttactgaa actgcctttc accattccgg
    22501 actcacgatt aagtacgtga gtgctactgt tgggcacaac ccaaatggtc aacgcaatgc
    22561 cggttgttgc cagaatagcg atcatccaga acagcgcgtg cagcccaagt ttgtgagtga
    22621 tgatcgggcc aagcaccatc gcaatggcaa aggtaatgcc aaagctcacg ccgataaacg
    22681 ccatcgcttt ggtgcggttt tgttcgcgcg tgagatcgga aagcagcgcc ataacggcgg
    22741 cggcaattgc tccagagcct tgtagcgccc ggcccagaat aattccccag atggagtcgg
    22801 aaagcgcagc gataacgcta ccggcggcaa acaccgccag cccaccgaca attaatggtt
    22861 tgcgaccaat gcggtcggaa agcaggccaa acggaatctg aaaaacggcc tgagtcagac
    22921 cataaatacc aatggcaata ccgattaatg cttcgctggc accttgcaga gccatgccgt
    22981 acgtggtcag aaccggcaga accataaaca tgcccagcat gcgcaacgag aatacggtcc
    23041 ctaaacccca ggtagcgcgc ctctcacctg gcgtcatttt ataatcgttc attaccaccc
    23101 cttaaaaaaa tgcccacata gtgtagtgcg gagcgcatgt tgggtaaaca gagtgttaat
    23161 gagaaaatat tacagaggag agtaatttgt gtgtcaggta tgtttttttg cattcataaa
    23221 atgaatgctc tatatgttga aaaaatgaat tttaaaacta aatagtttta catcaaaaaa
    23281 gtaataatat catttcttga ttgctatgtg cattttttat cttacgaata tgctagtttt
    23341 atctcactaa ataaacgatt tggtgacatg gatgataatg tttcaggcta aatgtgtgct
    23401 tattacttgg acctttatgg cactgtattt attcattaaa tggatgtttg gtggttaatt
    23461 attatggaaa tatatgacag tgaggacata aaggtataca tgttaactaa aagaatatta
    23521 ttttctgtaa tgctgatagt ttcaccaagc gtggtggcca gcgagaaagc gcatgaattg
    23581 tatgacagca tatacggtgg taaacctgct cccgatgtta taaatacact gcataaaatg
    23641 gctgaatcgg gagatatcga cgcgcagagc ctgttaggct gggaatatta tcagcctcgt
    23701 tatgatacca aacctgatgt tcaggaagcg attaaatggt tcgagttagc ggctaaacaa
    23761 ggtgacagag aagctccgtt agcgctgggg ggtatctact acgacggtga gcaggtgcgg
    23821 gtggattatg ccaaagcgta tgcactattt aatcaggccg cgcagcacgg tgtgaattta
    23881 gcatggtcca ggttgggcat tatgtacgcc aatggtcagt atgttgaggt agattgtaag
    23941 aaagcgaagg aatatcttga taaaggtgtc cacatttatg gtggcccaga aaactttctg
    24001 gctacttgtc gaaaagacat gattgacaga aaaacggttg acgatacgtt acccgtgatt
    24061 acggttaccc gttctgggat gcgagataat tttttagata aaggttttcc tgcatggata
    24121 gcctcttcgc caccaccaat aaattaggcg aagtggccaa tcttcgcgtg acattcagca
    24181 ttcgtcgccc atccggtaag gagattaacc aaacggttgg cttcgcgcct ttcggtttaa
    24241 accggctgaa tattagcttt actgattacc tatttggttc atttaccagt aattcgtctc
    24301 ttattcttta taaaccggaa tttgagcgaa aatcttgtgc aactgtaagg accacaatcg
    24361 ttgccgcaac ggcaaccatt aacggcaagg atgtggagtt gctgaaagcg ggggcaattg
    24421 aacaaaagtg gtaacgctgc tcagcccccg gctgaggcaa aacttgccct ggcgggtggc
    24481 gataataatc taagccgcga gaatatgctg aatcgccaaa aacataggta cagcaccaaa
    24541 agcatttggt gtcgctgaaa gctgaacaaa gccgagacga gcataaaaat taatggcggc
    24601 agggtcagca tcaagataaa cccctttaat tggtaatgcc tggtgaatta tttttacatg
    24661 ttcaaaaaaa tcacatagta ggtcctgacc aaagccccgc ttttgatact tccgtgctac
    24721 ccccaacatg actaatctga caacaccaat ttctgaaggt tgtgaaccct gaaggacgcc
    24781 agaaacgcgt tgtttttcca gcgaatatgc cgtaaaagta cagatgccga tcagttcacc
    24841 actttgtctg tcaataagtg ctttagccgc acagtcgcta ttacgcacac ttttctttag
    24901 cgatgcgcgt acaaatttat cgataacagg gttaccgcaa tcaaactgtt tttgcccagg
    24961 ataagtaata tcagactgat aattacagga gaggactatg ggcagagtaa tctcttcatg
    25021 cttgtcgacc atcgctgttt ttccttttca ttaacgcctt gagggcgagc gtcggttgag
    25081 caggttcagc gatgagttgg ttcatcagtt cccagctttg attcgaaagc tcacggcgac
    25141 gttggttatc aagcacactt tcggcgcgtt ccatagcagc gctcaaaata aaagcgctta
    25201 aatctacccc aacggcttgc gcagcttcac gaagtttgtt tttggcgtcg ggactagttt
    25261 tgagttcgac ccgcgcattt ttcagcgttt tatcgacatc gggtagagtg gctgtttttc
    25321 caggcattat gacctctttg attaactgaa tgaaatcgta gggagtcgat ctcatacata
    25381 atacggctat caaccgtatg attaatttat gttcatctgc tcactctgtc aagacatacg
    25441 gttattagcc gtatctctga tctcagacaa taaaaaaggt gccgtagcac ctttttaata
    25501 gagaggtttt gttaccacac agcagccagc agcgtatgcg agtccggtac cataaaatca
    25561 acggacatca tcaccgagag ggcagtgatg gcgatgatag agaagccgaa cagcttgcgc
    25621 gcccagattc tgtcatcagc aactttataa ccgcgcagag ccatacctaa ccaccagacg
    25681 ctaaccgccg cggcgaccac cagatattta tatccagcgt aaccgccaag agagagcatc
    25741 agcgtggcaa cggcaaaggc gatgatatac agcgtgatgt gattcttcgc caccgaaatg
    25801 ccttttacca ctggcaatac cggaatgttt gccgcctggt aatccttaaa gcggaaaatg
    25861 gcgatggcat aggagtgagg catctgccac aggctgaaga tagccagcag gatcgctgcg
    25921 ccgctatcga actcgccggt tactgcacag tagccaatca ccggcggcgc agcgccggag
    25981 agcgaaccaa tcaatgtgcc gtagactgag tggcgtttca tgtacaggct atagacgccg
    26041 acataaacca caaagcccat cacccccagc cagcaggcca gcggattcgc gccaaaccac
    26101 agcagcataa agccagcaat acccagcaag gtggcgtaca ccagcgagac agcaggagag
    26161 atcaggcctt taaccagcac ccgattcttc gtcctttcca tctttctgtc gatatccctg
    26221 tcgatgtagt tgttaaacac acaacccgac gccacaacca gtgacacccc aaccagcgtg
    26281 tagataaaca ggggataatc aatgctgccc tttgaggcca gcaggaatcc cccaatcacc
    26341 gagatcaggt tgccaaagat gatgcctggt ttcgttactt gcaggtattg cttaaacatc
    26401 ataaccgccg ctcttagtgc atcatcatgt tgtagttgag gttccacata atccagatgg
    26461 agcctacaac caggatagcg atgattagca cggtgaagac aaacgccgtc atgttccagc
    26521 cttcatctga tttggtattc atgtgcagga agcacaccag atgcaccaga acctgtacca
    26581 ctgccattgc caggattgtt cccagaatta cggccggaga ggcagcccct gtcatcacca
    26641 tccagaacgg aatcaccgtc agaatgatcg acaggataaa gcctgtcatg taggttttta
    26701 cgctgccatg ggacgcgccg ctgtgatcgg tagaatgact cattacatcg cccccatcag
    26761 ataaacaaca gtgaacacac agatccaaac cacatccagg aagtgccaga acaggctcag
    26821 gcacatgatg cgggtacggt tagtgctggt caggccgcga cgagcgattt gtaccatcag
    26881 caccgccatc cagataagac cggaagtgac gtgcagaccg tgcgtgccga ccagcgcaaa
    26941 gaacgctgac aggaagccgc tgcgatccgg acccatgccg ttaacaatca ggtgatggaa
    27001 ttcgtagatt tccatcccga taaatccggc accaaacaac caggtcaacg ccagccagga
    27061 gataacctgg cttttgttgt ttttgtacat ggcgatagcc gccatgccgt aggtgatgga
    27121 gctgaacaac agcaagaaag tttcaaccag aacgaacggc agttcgaaaa tgtccttacc
    27181 tgtcgggccg cctgcggtgc cgttcaccag aacggcatag gtagcaaaca agattgagaa
    27241 cagaatgcag tcgctcatca ggtagatcca aaatccgaag attttggttc cgcctgcatc
    27301 gtggtgcccg tgttcgtgcg cgtgggcagt cgcgtgcgtc aaagtatcag ttgccatttt
    27361 tcagccctgc cttagtaatc tcatcgaaat gctggttttc cagtttttcg atttctgcca
    27421 ccggcacgta gtaatccacg tcctcgtcga agcttttcac gatccaggtg atgatcatgc
    27481 ctgcgaagcc aacaatcgcc agccaccaga tatgccagat catggcgaaa ccgaagatgg
    27541 tggagaaagc tgcaatgacg atacctgcac cgctgttttt cggcatatga atttcttcat
    27601 agtggtcagg ctttttatac gcttcgcctt tctctttcat ttcccagaat gcatcacgct
    27661 cgtgaacgtg cggcacaacg gcaaagttat agaacggagg cggggaagag gttgcccact
    27721 ccagcgtacg gccaccccac gggtcgccag tcaggtcacg gttctggtcg cggtcgcgaa
    27781 tagaaacgta catctgaata acgaggcaga gaatacccag cgcaatcagt actgcaccgc
    27841 tagctgcaat catcagcatg gtgtggaact gcgggtcaat ctgctggctc aaacgacggg
    27901 tcatgcccat gaagcccagc gcatacagcg gcataaaggc aacgaagaag ccgatgatcc
    27961 agaaccagaa cgcgcgttta ccccaggttt cattcagttt gaaaccgaag gctttaggcc
    28021 accagtaggt catccctgcg aagcagccga agaccacgcc gccgatgatc acgttatgga
    28081 agtgcgcaat caggaacagg ctgttatgca gaacgaagtc cgcgcccggt actgccagca
    28141 gcacgccagt catcccgccc accgagaaag tgacgataaa accgatggtc cacagcatcg
    28201 cagaatggaa cacgatgcgg ccctgataca tggtgaacag ccagttgaag atcttcaccc
    28261 cggtcgggat ggcgataatc attgtggtga taccaaagaa ggcgtttacg ttcgcgcccg
    28321 cacccatcgt aaagaagtgg tgcagccaaa cgatgaacga cagcacggtg atacagacgg
    28381 ttgcccatac cagcgaggta taaccaaaca gacgtttacg cgagaaggtt gccgcaattt
    28441 cggagaacac accgaaaaca ggcaggatca ggatgtaaac ttccgggtgg ccccaggccc
    28501 aaatcaggtt gatgtacatc atcatgttgc cacccatatc gttggtaaag aaatgggtgc
    28561 ccagatagcg atccagggtc aacaacgcga cggtaaccgt cagaattggg aaggaagcaa
    28621 taatcagtac gttcgcgcac agtgatgccc aggtaaatac tggcatcttg aacatggtca
    28681 tgcccggtgc gcgcatcttc agaatggtaa cgaagaagtt gataccggta agcgtcgtac
    28741 ctataccgga tagctggaga ctccatatcc agtaatcgac accgactccc ggactgtact
    28801 ctattcccga tagcggtgga taggccagcc agccggtctg cgcaaattcg cccacgccga
    28861 gagaaacgtt aaccagaatc acaccaacaa cagtaaacca gaagcttaag ttgttgagga
    28921 acgggaacgc aacgtcacgc gcgccgatct gcagcggaac caccaggttc atcagaccga
    28981 taacgaaagg catcgccacg aagaagatca taatcacgcc gtgcgcggtg aagatctgat
    29041 cgtagtggtg aggtggcagg aagcccgctt cgcccgccga ggcaagagcc tgctggctac
    29101 gcatcataat ggcgtcagca aaaccacgca gcaacatcac aatcgctacg atgatataca
    29161 tgataccgag gcgtttatgg tcgacggagg tcagccactc tttccacagg taggtccact
    29221 taccgaagta agtgatcagg ccaacgagcg ccagacctcc caaaataatg ccagcgatcg
    29281 taaccatgac gataggttca tggaacggga ctgcatcaag tgataatttt ccgaacatct
    29341 ttattcttcc tcaacccctt taatgggcgg attccgcgtg gctcatgtcc atgccttcca
    29401 taccttcgtg tgcactgtgc tcaccttctg gctgggtcat gtccatgctc ttaccgtgag
    29461 ccataaactt gttaatcaca tcggcaaaca agtctggttt cacgttggag aaatattcca
    29521 cctggttgta ttcgctaggc gcggccagtt tttcgaacgc agccatgtca gacatggtgt
    29581 tcggcgactg tttcgctttt gcgacccatt ggtcgaattc ggcgcgatcc ggtgttgcaa
    29641 tagctttgaa cttcatgcct gagaagcccg gcccgctgta gctggcggag ataccgtcat
    29701 aagtaccggg ttcgttggcg atcagatgca ggcgagtctg cataccggcc atggcataaa
    29761 tctggctacc cagacgcgga atgaagaagg agttcatcac ggagttggag gtcactttga
    29821 agtacaccgg agtgttcgcc gggaaagcga tttcattcac ggtagcaatg ccctgttccg
    29881 ggtagatgaa gaaccatttc cagtccatgg aaaccacttc gatggtaatg ggcttctcgt
    29941 cgtgtgccag cggcttgcta ggctcaagag cgtgagtggt tttccaggtc agtaccgcaa
    30001 ggaagatgat gattaagata ggtaccgtcc agaccacagc ttccacttta ttggagtgtg
    30061 accagttcgg gctgtactta gcatctttat tgctcgcacg atacttccag gcgaaaccaa
    30121 cagccatcaa gattgcggga ataacgacaa tcaacatcag gccaaatgcc gtcagtatca
    30181 gtgaacgttg ctccagacca atctgtcctt tgggatctaa cagcgcagaa ttacagccac
    30241 tgagcaatac agtgcctgca aataatgaca accatcccaa acttttattg tatttcctga
    30301 gtctcattta acgacctcaa ttccacggga tctggtggcg tttaaagtgt gtgggcattt
    30361 tacgggaagg ttacattact gtaaacatga ttaaattcct gttacttgga tttggctgtc
    30421 aggtcacata tgttgcaaaa catatcaagt tgattttaag aagctgttgc aaaagggcgt
    30481 tataacgaaa ggggagtgca gaaggagttt ggacgggcaa ttggtataac caatctggaa
    30541 taaaacaatt gtttaacaaa taattatcat tatggtgaca attgggtgga ggggaaaata
    30601 aagactcatt agctgaatcg tgtaagaaaa aatagaattc ataaattgcg tcaataattt
    30661 ccaaacgcta agccgcacaa aagaacaaat attattaagc gatactgatg tttatttagc
    30721 cgtaataatt acggcgagtg attactacag ctaaataata tttacagatt acgtcagatg
    30781 cgtttttcgt agcgccagat aatccagcaa accaccaagc acgacgccag aaagggcgac
    30841 taaaaccccg acttccagca gagcaggcag gaaagagaag tgcgtcaaat ccagcgcgtc
    30901 catcgtcaac agcagtaacc acacggccaa caggctgacg cccgccgcca gtgtccacat
    30961 ggcaaaggca taacctgccg gatattcggt acgggagata aagttgtcat ttactcgtgt
    31021 atattcaagc gtctggcggc aaaccagcag caaaataagc cccggtacgg cagcggcgac
    31081 ggagaataga tagaatgtcg accagccgtg tgcttcaaca aaccaacccg ccacggggcc
    31141 gacataaact cgccctacag cggaaagcgc tgagagcagg gcaaattgag ttgcggaaaa
    31201 tgacttatta cacagcgtca ttaacagcgc gacaaaggct gatgtgccca tcccgccaca
    31261 gaggttttcg aaaaagacgg ctgcgcccat gctgtagaga tgcttatcag taatcgacag
    31321 cagccagtaa ccagcgttag acgcaccttg taaaatgccg aaaatcagca gcgcccggaa
    31381 cagcgacagg cgctgcatca aaatcccacc gtacaatgcg cccacaatgg tcgccagtaa
    31441 gccaagcgtt ttgttaacca cgcctacttc acccgcatca aacccgacgc cgcgaatcaa
    31501 aaacgtggtt gtcaggctca tggcgaatgc gtcgcccagc ttatacagca cgataagaag
    31561 caaaataagc caggcattat tgcgaccaaa gaaatctcgc agaggtgcaa caaccgcttg
    31621 ttccagcgtt tttggcacag gaatggtgtc ggtcggttct ggtgcaagca acgtcgcgat
    31681 aatacaaggg atcaacagcg ccgccatcaa ccagtacatg ccctgccagc ccagccattt
    31741 atctgccagc cacagggcta agccgccgga aaccagcatc cctaaacggt aacccagcac
    31801 gctgattgcc gcgcccgcac cacgttcttc tgccgggagc acatcggttt tccacgcatc
    31861 gaagacaata tcctgggagg ccgagcaaaa agcgatcacc accgccagtg ccgccatcca
    31921 gcggagttgg gtgccaggtt cgagaaaacc catcgccgca atggcgacta ataacaggat
    31981 ttgcgtggcg agcagccaac cgcgccgccg cccaaaaaat gggggcgtgt agcggtccat
    32041 cagcggtgac cagaggaatt taaaaacgta agcctggcct accagagaga agaaaccaat
    32101 ggttttgaga tcaatattct cgaccgtcat ccaggcctgt aaggtgccgg atgtcagggc
    32161 gagcggtagc ccggaagcaa aacccaggat cagcaatatg gctgaacgcg gctgttgaaa
    32221 aatacgtaaa tattgactgg acatgggcgt ctacaggccc ggtttgcacc gggccagagg
    32281 gcagaaatta acgcgcgttc tgcttgataa attcgtgaat gctggtgtcc tgagacatat
    32341 cagcgatggt atcggtcagc acactgttaa ccgcatcggc gatatttttg ttggaggcct
    32401 ggaacgcacc ttcaacgttg tagctggcac gatagttttt ggtcattttg ttgccattct
    32461 gcgcggtagc gatgatggcg atatccgctt tggtcgcgat gttatagcgc acgttgccct
    32521 gggacacgtc agcatacagt tggctaacga tgatttgcag attaaccggg ccattcggac
    32581 caaccatgta accacgcgcg gtcatctgtt tttccagcac ttcttgcagc aggaaacgca
    32641 gatcgcggga ggcggtcagg gtaacgattt gattatcgcg ggtgactttt gccagcgcct
    32701 gatcggtacg ctgatcggca ccattaatgc ttacggtgac gcccatcagg cttggatcct
    32761 gctgtggcag tgtaatcgtc ggggaaactt caatagttgt tggcggtttt gcgcatcctg
    32821 caagcataaa cagagcaact aacgggaaga ggattttttt gaacatgttc gggctctcag
    32881 agactcttaa gcgtgtttgg taaaaattcc cgccatcata acattgccaa cggcgagggg
    32941 aagtgggtaa ggcatgtaaa ttcatcatgt tgacgaaata atcacccctg gtaaaagaaa
    33001 cgctgatgcg aggcctgtgt ttcaatcttt aaatcagtaa acttcatacg cttgacggaa
    33061 aaaccaggac gaaacctaaa tatttgttgt taagctgcaa tggaaacggt aaaagcggct
    33121 agtatttaaa gggatggatg acatctcagc gttgtcggag gagatatttc atgatgatac
    33181 gtgagcggat agaagaaaaa ttaagggcgg cgttccaacc cgtattcctc gaagtagtgg
    33241 atgaaagcta tcgtcacaat gtcccagccg gctctgaaag ccattttaaa gttgtgctgg
    33301 tcagcgatcg ttttacgggt gaacgttttc tgaatcgtca tcgaatgatt tacagtactt
    33361 tagcggagga actctctact accgttcatg cgctggctct gcatacttac actattaagg
    33421 agtgggaagg gttgcaggac accgtctttg cctctcctcc ctgtcgtgga gcaggaagca
    33481 tcgcgtaaaa acgcatttgc aactgtcggc gcttttccag tatgttgcta aagattttat
    33541 gaaaaacggc ctgcgggccg ttttgttttg tctggatttt gcgctttttg cccagcattc
    33601 agacgaaaat tgcccgggaa ttgtgaaaaa atacgcgaca gcgcgcaata accgttctcg
    33661 actcataaaa gtgatgccgc tataatgccg cgtcctattt gaatgctttc gggatgattc
    33721 tggtaacagg gaatgtgatt gattataaga acatcccggt tccgcgaagc caacaacctg
    33781 tgcttgcggg gtaagagttg accgagcact gtgatttttt gaggtaacaa gatgcaagtt
    33841 tcagttgaaa ccactcaagg ccttggccgc cgtgtaacga ttactatcgc tgctgacagc
    33901 atcgagaccg ctgttaaaag cgagctggtc aacgttgcga aaaaagtacg tattgacggc
    33961 ttccgcaaag gcaaagtgcc aatgaatatc gttgctcagc gttatggcgc gtctgtacgc
    34021 caggacgttc tgggtgacct gatgagccgt aacttcattg acgccatcat taaagaaaaa
    34081 atcaatccgg ctggcgcacc gacttatgtt ccgggtgaat acaagctggg tgaagacttc
    34141 acttactctg tagagtttga agtttatccg gaagttgaac tgcagggtct ggaagcgatc
    34201 gaagttgaaa aaccgatcgt tgaagtgacc gacgctgacg ttgacggcat gctggatact
    34261 ctgcgtaaac agcaggcgac ctggaaagaa aaagacggcg ctgttgaagc agaagaccgc
    34321 gtaaccatcg acttcaccgg ttctgtagac ggcgaagagt tcgaaggcgg taaagcgtct
    34381 gatttcgtac tggcgatggg ccagggtcgt atgatcccgg gctttgaaga cggtatcaaa
    34441 ggccacaaag ctggcgaaga gttcaccatc gacgtgacct tcccggaaga ataccacgca
    34501 gaaaacctga aaggtaaagc agcgaaattc gctatcaacc tgaagaaagt tgaagagcgt
    34561 gaactgccgg aactgactgc agaattcatc aaacgtttcg gcgttgaaga tggttccgta
    34621 gaaggtctgc gcgctgaagt gcgtaaaaac atggagcgcg agctgaagag cgccatccgt
    34681 aaccgcgtta agtctcaggc gatcgaaggt ctggtaaaag ctaacgacat cgacgtaccg
    34741 gctgcgctga tcgacagcga aatcgacgtt ctgcgtcgcc aggctgcaca gcgtttcggt
    34801 ggcaacgaaa aacaagctct ggaactgccg cgcgaactgt tcgaagaaca ggctaaacgc
    34861 cgcgtagttg ttggcctgct gctgggcgaa gttatccgca ccaacgagct gaaagctgac
    34921 gaagagcgcg tgaaaggcct gatcgaagag atggcttctg cgtacgaaga tccgaaagaa
    34981 gttatcgagt tctacagcaa aaacaaagaa ctgatggaca acatgcgcaa tgttgctctg
    35041 gaagaacagg ctgttgaagc tgtactggcg aaagcgaaag tgactgaaaa agaaaccact
    35101 ttcaacgagc tgatgaacca gcaggcgtaa tttacgcagc ataacgcgct aaattcgcac
    35161 aaaggcccgt caccgccagg tggtgggctt ttttttgtca tgaattttgc atggaaccgt
    35221 gcgaaaagcc tctttcggtg ttagcgtaac aacaaaagat tgttatgctt gaaatatggt
    35281 gatgccgtac ccataacaca gggactagct gataatccgt ccataaggtt acaatcggta
    35341 cagcaggttt tttcaatttt atccaggaga cggaaatgtc atacagcggc gaacgagata
    35401 actttgcacc ccatatggcg ctggtgccga tggtcattga acagacctca cgcggtgagc
    35461 gctcttttga tatctattct cgtctactta aggaacgcgt catttttctg actggccagg
    35521 ttgaagacca catggctaac ctgattgtgg cgcagatgct gttcctggaa gcggaaaacc
    35581 cagaaaaaga tatctatctg tacattaact ccccaggcgg ggtgatcact gccgggatgt
    35641 ctatctatga caccatgcag tttatcaagc ctgatgtcag caccatctgt atgggccagg
    35701 cggcctcgat gggcgctttc ttgttgaccg caggggcaaa aggtaaacgt ttttgcctgc
    35761 cgaattcgcg cgtgatgatt caccaaccgt tgggcggcta ccagggccag gcgaccgata
    35821 tcgaaattca tgcccgtgaa attctgaaag ttaaagggcg catgaatgaa cttatggcgc
    35881 ttcatacggg tcaatcatta gaacagattg aacgtgatac cgagcgcgat cgcttccttt
    35941 ccgcccctga agcggtggaa tacggtctgg tcgattcgat tctgacccat cgtaattgat
    36001 gccagaggcg caactgtgcc gctatactta tccagggcgg cacaacgctg taagcggctt
    36061 gcgcctgaga atggcatttg cgtcgtcgtg tgcggcacaa agaacaaaga agaggttttg
    36121 acccatgaca gataaacgca aagatggctc aggcaaattg ctgtattgct ctttttgcgg
    36181 caaaagccag catgaagtgc gcaagctgat tgccggtcca tccgtgtata tctgcgacga
    36241 atgtgttgat ttatgtaacg acatcattcg cgaagagatt aaagaagttg caccgcatcg
    36301 tgaacgcagt gcgctaccga cgccgcatga aattcgcaac cacctggacg attacgttat
    36361 cggccaggaa caggcgaaaa aagtgctggc ggtcgcggta tacaaccatt acaaacgtct
    36421 gcgcaacggc gataccagca atggtgtcga gttgggcaaa agtaacattc tgctgatcgg
    36481 tccgaccggt tccggtaaaa cgctgctggc tgaaacgctg gcgcgcctgc tggacgtccc
    36541 gttcaccatg gccgacgcaa ccacgctgac cgaagccggt tatgtgggcg aagacgttga
    36601 aaacatcatt cagaagctgt tgcagaagtg cgattacgac gtacagaaag cgcagcgcgg
    36661 gattgtctac atcgatgaaa tcgacaagat ttctcgtaag tcagacaacc cgtccattac
    36721 ccgagacgtt tccggtgaag gcgtacagca ggcactgttg aaactgatcg aaggtacggt
    36781 agctgctgtt ccaccgcaag gtgggcgtaa acatccgcag caggaattct tgcaggttga
    36841 tacctctaag atcctgttta tttgtggcgg tgcgtttgcc ggtctggata aagtgatttc
    36901 ccaccgtgta gaaaccggct ccggcattgg ttttggcgcg acggtaaaag cgaagtccga
    36961 caaagcaagc gaaggcgagc tgctggcgca ggttgaaccg gaagatctga tcaagtttgg
    37021 tcttatccct gagtttattg gtcgtctgcc ggttgtcgca acgttgaatg aactgagcga
    37081 agaagctctg attcagatcc tcaaagagcc gaaaaacgcc ctgaccaagc agtatcaggc
    37141 gctgtttaat ctggaaggcg tggatctgga attccgtgac gaggcgctgg atgctatcgc
    37201 taagaaagcg atggcgcgta aaaccggtgc ccgtggcctg cgttccatcg tagaagccgc
    37261 actgctcgat accatgtacg atctgccgtc catggaagat gtcgaaaaag tggttatcga
    37321 cgagtcggta attgatggtc aaagcaaacc gttgctgatt tatggtaagc cggaagcgca
    37381 acaggcatct ggtgaataat taaccattcc catacaatta gttaaccaaa aaggggggat
    37441 tttatctccc ctttaatttt tcctctattc tcggcgttga atgtggggga aacatcccca
    37501 tatactgacg tacatgttaa tagatggcgt gaagcacagt cgtgtcatct gattacctgg
    37561 cggaaattaa actaagagag agctctatga atcctgagcg ttctgaacgc attgaaatcc
    37621 ccgtattgcc gctgcgcgat gtggtggttt atccgcacat ggtcatcccc ttatttgtcg
    37681 ggcgggaaaa atctatccgt tgtctggaag cggcgatgga ccatgataaa aaaattatgc
    37741 tggtcgcgca gaaagaagct tcaacggatg agccgggtgt aaacgatctt ttcaccgtcg
    37801 ggaccgtggc ctctatattg cagatgctga aactgcctga cggcaccgtc aaagtgctgg
    37861 tcgaggggtt acagcgcgcg cgtatttctg cgctctctga caatggcgaa cacttttctg
    37921 cgaaggcgga gtatctggag tcgccgacca ttgatgagcg ggaacaggaa gtgctggtgc
    37981 gtactgcaat cagccagttc gaaggctaca tcaagctgaa caaaaaaatc ccaccagaag
    38041 tgctgacgtc gctgaatagc atcgacgatc cggcgcgtct ggcggatacc attgctgcac
    38101 atatgccgct gaaactggct gacaaacagt ccgttctgga gatgtccgac gttaacgaac
    38161 gtctggaata tctgatggca atgatggaat cggaaatcga tctgctgcag gttgagaaac
    38221 gcattcgcaa ccgcgttaaa aagcagatgg agaaatccca gcgtgagtac tatctgaacg
    38281 agcaaatgaa agctattcag aaagaactcg gtgaaatgga cgacgcgccg gacgaaaacg
    38341 aagccctgaa gcgcaaaatc gacgcggcga agatgccgaa agaggcaaaa gagaaagcgg
    38401 aagcagagtt gcagaagctg aaaatgatgt ctccgatgtc ggcagaagcg accgtagtgc
    38461 gtggttatat cgactggatg gtacaggtac cgtggaatgc gcgcagcaag gtcaaaaaag
    38521 acctgcgtca ggcgcaggaa atccttgata ccgaccatta tggtctggag cgcgtgaaag
    38581 atcgcatcct tgagtacctt gcggttcaaa gccgtgtcaa caaaatcaag ggaccgatcc
    38641 tctgcctggt agggccgccg ggggtaggta aaacctctct tggtcagtcc attgccaaag
    38701 ccaccgggcg taaatatgtc cgtatggcgc tgggcggcgt gcgtgatgaa gcggaaatcc
    38761 gtggtcaccg ccgtacttac atcggttcta tgccgggtaa actgatccag aaaatggcga
    38821 aagtgggcgt gaaaaacccg ctgttcctgc tcgatgagat cgacaaaatg tcttctgaca
    38881 tgcgaggcga tccggcctct gcactgcttg aagtgctgga tccagagcag aacgtagcgt
    38941 tcagcgacca ctacctggaa gtggattacg atctcagcga cgtgatgttt gtcgcgacgt
    39001 cgaactccat gaacattccg gcaccgctgc tggatcgtat ggaagtgatt cgcctctccg
    39061 gttataccga agatgaaaaa ctgaacatcg ccaaacgtca cctgctgccg aagcagattg
    39121 aacgtaatgc actgaaaaaa ggtgagctga ccgtcgacga tagcgccatt atcggcatta
    39181 ttcgttacta cacccgtgag gcgggcgtgc gtggtctgga gcgtgaaatc tccaaactgt
    39241 gccgcaaagc ggttaagcag ttactgctcg ataagtcatt aaaacatatc gaaattaacg
    39301 gcgataacct gcatgactac ctcggtgttc agcgtttcga ctatggtcgc gctgataacg
    39361 aaaaccgtgt cggtcaggta accggtctgg cgtggacgga agtgggcggt gacttgctga
    39421 ccattgaaac cgcatgtgtt ccgggtaaag gcaaactgac ctataccggt tcgctcggcg
    39481 aagtgatgca ggagtctatt caggcggcgt taacggtggt tcgtgcgcgt gcggaaaaac
    39541 tggggatcaa ccctgatttt tacgaaaaac gtgacatcca cgtccacgtt ccggaaggtg
    39601 cgacgccgaa agatggtccg agtgccggta ttgctatgtg caccgcgctg gtttcttgcc
    39661 tgaccggtaa cccggttcgt gccgatgtgg caatgaccgg tgagataact ctgcgtggtc
    39721 aggtactgcc gatcggtggt ttgaaagaaa aactactggc agcgcatcgc ggcgggatta
    39781 aaacagtgct aattccgttc gaaaataaac gcgatctgga agagattcct gacaacgtaa
    39841 ttgccgatct ggacattcat cctgtgaagc gcattgagga agttctgact ctggcgctgc
    39901 aaaatgaacc gtctggcatg caggttgtga ctgcaaaata gtgacctcgc gcaaaatgca
    39961 ctaataaaaa cagggctggc aggctaattc gggcttgcca gccttttttt gtctcgctaa
    40021 gttagatggc ggatcgggct tgcccttatt aaggggtgtt gtaaggggat ggctggcctg
    40081 atataactgc tgcgcgttcg taccttgaag gattcaagtg cgatataaat tataaagagg
    40141 aagagaagag tgaataaatc tcaattgatc gacaagattg ctgcaggggc tgatatctct
    40201 aaagctgcgg ctggccgtgc gttagatgct attattgctt ccgtaactga atctctgaaa
    40261 gaaggggatg atgtagcact ggtaggtttt ggtacttttg ccgttaaaga gcgtgctgcc
    40321 cgtactggcc gcaacccgca gaccggtaaa gagatcacca tcgctgccgc taaagtaccg
    40381 agcttccgtg caggtaaagc actgaaagac gcggtaaact aagcgttgtc cccagtgggg
    40441 atgtgacgaa gttcaagggc gcatctactg atgtgccttt tttatttgta ttcggtgact
    40501 ttctgtgtct tgtgggctga caattgcccc cgtttcttgt cacaataggc ctttgcgcgc
    40561 atcgatacgt tgcgtgaggt acacagtcat ctacagcgga gtgttgttac accatgatgg
    40621 acagcttacg cacggctgca aacagtctcg tgctcaagat tattttcggt atcattatcg
    40681 tgtcgttcat attgaccggc gtgagtggtt acctgattgg cggaggcaat aactacgccg
    40741 caaaagtgaa tgaccaggaa atcagccgtg ggcagttcga gaacgccttt aacagcgagc
    40801 gtaatcgcat gcagcaacag ctgggcgatc aatactccga gctggcagcg aacgaaggct
    40861 atatgaaaac cctgcgtcaa caggtgctga atcgtctgat cgacgaggcg ctgttggatc
    40921 agtacgctcg tgagctgaaa ctgggtatca gtgatgagca ggttaaacag gcgattttcg
    40981 cgaccccagc cttccaggtt gacggcaaat ttgataacag ccgctataac ggtatcctca
    41041 accagatggg gatgaccgcc gatcagtacg cccaggcgct gcgtaaccag ctcactaccc
    41101 aacagctgat taacggtgtt gccggtaccg attttatgct gaaaggtgaa accgacgagc
    41161 tggcggcact ggtcgcgcaa caacgcgtgg tgcgtgaggc gactatcgat gttaacgcgc
    41221 tggcggcgaa gcagcctgtg accgaacagg agattgccag ctactacgaa caaaacaaaa
    41281 acaatttcat gacgccggaa caattccgcg tgagttacat caagctggat gccgcaacga
    41341 tgcagcaacc ggttagcgat gcggatatcc agagctacta cgatcagcat caggatcaat
    41401 tcacccagcc gcagcgtacc cgctacagca tcatccagac caaaactgaa gatgaagcga
    41461 aagcggtact tgatgagctg aataaaggcg gtgattttgc tgcattagcc aaagaaaaat
    41521 ctgccgatat tatctctgcc cgtaacggcg gcgatatggg ttggttagaa gatgccacta
    41581 tccccgacga actgaaaaat gctggtctga aagaaaaagg gcaactgtct ggtgtcatca
    41641 aatcttcggt cggtttcctg attgtacgtc tggacgacat tcagccagcg aaagtgaaat
    41701 cgttagacga agtacgtgac gacattgcgg cgaaagtgaa acacgaaaaa gccctcgatg
    41761 cgtactatgc gctgcagcag aaagtgagcg atgcggcaag caacgacacc gagtctctgg
    41821 ccggtgcaga gcaagctgct ggcgttaaag ccactcagac gggttggttc agcaaagata
    41881 acctgccgga agagttgaac ttcaagccgg ttgccgacgc tatctttaac ggcggtctgg
    41941 taggtgaaaa cggcgcgccg ggcatcaact ctgacatcat caccgtagac ggcgaccgcg
    42001 cattcgtgct gcgcatcagc gagcacaaac cggaagcggt gaaaccgttg gcagatgttc
    42061 aggaacaagt taaggcattg gttcagcaca acaaagctca acaacaggcg aaagtggatg
    42121 ctgagaaact gctggttgat ttgaaagccg gcaaaggtgc ggaagctatg caggctgccg
    42181 gtctgaaatt tggcgagccg aaaaccttaa gccgttccgg tcgtgacccg attagccagg
    42241 cggcgtttgc actgccactg ccagcgaaag acaaaccgag ctacggtatg gcgaccgata
    42301 tgcaaggtaa tgtggttctg ctggcgctgg atgaagtgaa acaaggttca atgccggaag
    42361 atcagaaaaa agcgatggtg cagggtatca cccagaacaa cgcacaaatc gtctttgaag
    42421 ctctgatgag taacctgcgt aaagaggcga aaatcaaaat tggcgatgcg ctggaacagc
    42481 aataatcctg aagccgcctc gcaaaaaaat gcgttgcagc tgtaacaaga aaaggtcgct
    42541 ttcgcggcct tttccatttc tgaacattgc catttgttta ctgttttcac tgccgttaag
    42601 gtgattccac tgttaacaaa caaggagaaa acagtatgaa acacggaatt aaagcactgc
    42661 tcattaccct gtccctggcc tgtgccggaa tgtcttatag cgcgctggcg gcagcttctg
    42721 tggcgaaacc gacaacggta gaaaccaaag cggaagctcc tgcagcacaa agtaaagcag
    42781 cagtaccggc gaaagccagt gacgaagaag gcacccgggt cagcattaat aatgccagcg
    42841 cggaagagct agcccgcgcg atgaatggcg ttggcctgaa gaaagcgcag gcgattgtca
    42901 gttatcgcga agagtacggt ccgtttaaaa cggtggagga tctaaagcag gtgccgggga
    42961 tgggcaattc gctggtggaa cgtaatctgg cggtattaac cctgtaatta atttgcatag
    43021 tggcaatttt ttgccagact gaagaggtca taccagttat gacctctgta cttataacaa
    43081 caacgtaagg ttattgcgct atgcaaacac aaatcaaagt tcgtggatat catctcgacg
    43141 tttaccagca cgtcaacaac gcccgctacc ttgaatttct cgaagaagcc cgctgggatg
    43201 ggttggaaaa tagcgacagt tttcagtgga tgacggccca taacatcgcc ttcgtcgtgg
    43261 tcaatatcaa tattaactat cgtcgcccag cggtattaag tgacctgtta actattacca
    43321 gtcagttgca gcaattaaac ggtaaaagcg gcatcttaag ccaggtcatt acactggagc
    43381 cggaagggca ggtggtagcg gatgcgctta ttacgtttgt ttgtattgat cttaaaacgc
    43441 agaaagcatt agctctggaa ggggaattgc gcgaaaagct ggagcagatg gttaagtaaa
    43501 cgttttgtgg tgccggatgc tcaagccgca tccggcgaca cccggaataa ttacctcaac
    43561 ccggttttct gcttcatcgc tgccatcacc gtcggtttat cggccagata atgattcaaa
    43621 ccgttggcgc gtaaattaca tgccgcacaa tgaccgcaac cgtcgccttt aatgccgtta
    43681 tagcaggtca acgtttcgtt acggactaaa tccagtttgc cgtaataatc tgccagcgcc
    43741 caggtttccg ctttatcaat ccacatcagc ggcgtttcaa aacgaatatc tttcgccatg
    43801 cccaaactga cggcatggtt tagtgctttc acaaactcat cgcggcaatc cgggtagccg
    43861 gagaaatccg tttcacagac gccggtaatt acggcttctg cttttacctg atacgcatat
    43921 attgccgcca gcgtcaggaa caaaatatta cgccctggga caaacgtatt cgggatgcca
    43981 tcggcttcag gttcataatc aggcaccgga atgctgtcac gtgtcaggct gctgaccgcc
    44041 agctcgttga gcagcgttac atcaagaacc ttatgcgcgc gtgctcccag tttcagcgcc
    44101 agttcgcgtg ccacgtcgat ttctgcgcga tgtcgctgac cgtaatcgaa cgtcacgcaa
    44161 tggacttcat catattgttg taatgcctgc accagacagg tggtggaatc ctggcctcca
    44221 ctgaacacaa cgacagcacg tttcatagat aatcctgctt gaacaataaa agcgttatgg
    44281 taacgcctgc gattaacccg gaccagctat tcattgcgac ggcgcgggaa gccaggcttc
    44341 ggtaaattca aaccagccgc gcggtgtcag tcgcacaccg ttcacgccgg gaggggcact
    44401 aatgcgatag tgatagttga acagcggcgt cagcgtcgcg tctgccatta actggctaaa
    44461 aaccgctttc agggcattaa atcggttttc ctcatcaggc attacttgca ccgcgtccag
    44521 cgtcgattgc agatgtgcat atgctggagc gtcgaaaaca tgtggccata gtggatcgca
    44581 acgtagccat tgctccagag tatattccgg tgcttcgcca attaatctgt cgcccatcat
    44641 gaggtctgcg tgtgccagta gggtcgtgtc gtcccagttt tttgcgttat gaaaaataat
    44701 tgtgagttca cagccttccg ctgccagtgt cgcctgtagg cgttctgcca tggtatgaag
    44761 ttctatcggt aggtgataaa ccagcgtcaa ggttttcggt agtttgactt catccggtac
    44821 ttgccagtac ggaatagtcc agcctggcag taatgcatga ctggcggtga tcaggttttc
    44881 tccgacttct aacgtttgta ataaaccgga ttgatgaata atggagatca cttttcgcgc
    44941 ctgccagagg gagagtcggg gacttttgcg caacgttaaa tagcaaaaac ctaaactgat
    45001 gccactactg acctggctga cccgttgcag ctcctccggt ttgccgatgg tgatttgcac
    45061 gggatgccga caactgtttc ccaaatcttt ttcgaaaagc ggcggagtta tccagtactc
    45121 aaccgcttta agcagcggat gacgcaaatg gtaataatca tggctctcca ggcgcaccag
    45181 ctccgctgtg aattgtgtta agcgaaaagg accagtgccg atcagtggga attgcggatg
    45241 cgccagatgg ctgcaatagc tcgccagccg gtgcgcaagc cagtaatcgg ggcgatgtaa
    45301 aaagaaggtc agacactgcg gatgggtgac ttcaatacgc ttcacgctaa taaataattg
    45361 atccagtgct ggcagttgta acagcatcaa taatcgctgg tgtaagtgtg aggcttttac
    45421 tgcatcgccg ttatgccagt gtagggttga acgaagataa aagtcccagc gtaacccgtc
    45481 agtagaggtt tcccagtgat gcgctaaatc gccaatcggg cgctgagtat tattatcgaa
    45541 gcgggtcagg ccggaaaata tctgcccggc gagatgctgc tcggcacggc cgggcaaaaa
    45601 gcctggttgt agcggttcga gcgggcgata gtagggaata cgcaacgtgg gtgtatcgtt
    45661 ttgccattgt ccgcccataa acggctgtaa cagagtgcgc agctcacctg gggccagttg
    45721 cgccagctcc agcacatctt gctgctttcc ggtttccagt gcctgttcca tcatcgcatt
    45781 gcgtagcgat tccggcgtga ccagaaagcg taattgtccg cgctttccgc gtcctgactg
    45841 cgcctgccac tccagccatc ccgcctcctg tgcctgacgc aacagcgtac gaacatggcg
    45901 ttcgctgcaa aaacagcgtt cggccagttc gctgacggtg acggtttgcg gctttccggc
    45961 tgaaggttgc cacagacgtt gatactggtt aagacggttg agcaatcgca tataaacccg
    46021 gaacaatatt atttaactat tcactattac ttccgtatat atcaggtgat actcaatcac
    46081 cattaaccgt gtcacagagt ggagaaagaa atggctcgtc tggcagcatt tgatatggat
    46141 ggcactttat tgatgcctga ccatcattta ggtgagaaaa ccctctctac tttggcgcga
    46201 ctgcgtgaac gcgacattac cctcactttt gccacggggc gtcatgcgct ggagatgcag
    46261 catattctcg gggcgctatc gctggatgca tatttgatta ccggcaacgg aacgcgcgtg
    46321 cattctctgg aaggtgaact tttacatcgt gatgatttac ctgcggatgt cgcggagctg
    46381 gtgctgtatc agcaatggga tacccgagcc agcatgcata tcttcaatga cgacggttgg
    46441 tttaccggga aagagatccc tgcgttgttg caggcatttg tctatagcgg ttttcgttat
    46501 cagataatcg atgtcaaaaa aatgccactc ggcagcgtca ccaagatctg cttctgtggc
    46561 gatcacgacg atcttacacg cttgcagatc cagctatacg aagcattagg cgagcgtgca
    46621 catttgtgtt tttccgccac ggattgcctc gaagtgctgc cggtgggctg caataaaggc
    46681 gctgcattga cggtgctgac ccaacattta ggtttatcgt tgcgcgattg catggccttt
    46741 ggtgatgcga tgaacgatcg cgaaatgtta ggcagcgtcg gtagcgggtt tattatgggc
    46801 aatgcgatgc cgcaactgcg cgcggagctc ccgcatttac cggtgattgg acattgccga
    46861 aatcaggctg tctctcacta tttgacgcac tggctggact atccacatct accttattcc
    46921 cccgaataac gagatccctt ccagcaccgg gcaattgccc ggtttttttt gcgttgaatt
    46981 tgtcattttg tgccgtggtg tttaaaccgc acagaataaa ttgtcgtgat ttcaccttta
    47041 aaatagaatt aaaagagaaa aaaattctct gtggaagggc tatgttagat aaaattgacc
    47101 gtaagctgct ggccttactg cagcaggatt gcaccctctc tttgcaggca ctggctgaag
    47161 ccgttaatct gacaaccacc ccttgctgga agcgcctgaa acggctggag gacgacggta
    47221 tccttatcgg caaagtcgcc ctgctggatc cggaaaaaat aggcctcggc ctgaccgctt
    47281 ttgtgctgat aaaaacgcaa catcacagca gcgaatggta ttgccgcttt gtcacggtgg
    47341 ttaccgaaat gccagaagtg ctggggttct ggcgcatggc tggtgaatac gattatctga
    47401 tgcgcgtcca ggttgccgac atgaaacgct acgacgagtt ttataagcgt ctggtaaaca
    47461 gcgtgccggg gctgtcggac gtcacttcca gcttcgcgat ggaacagatt aaatacacca
    47521 cttctttacc catcgaataa atatccagaa tcaggtcagg acacaacgcg tgcgattatt
    47581 tgctcaatta agctggtatt tccgtcggga atggcgtcgc tatctcgggg ctgtcgcctt
    47641 gcttgtcatt atcgcgatgc tgcaactggt tccgccaaaa gtggttggta ttgttgtcga
    47701 tggcgtgaca gaacaacact ttactaccgg gcagatcctg atgtggatcg ccaccatggt
    47761 gctgattgcc gttgtggttt atctcctgcg ttacgtctgg cgggtattgc tgtttggtgc
    47821 gtcttatcaa ctggctgttg aactgcgtga agattattac cgtcagctaa gccggcagca
    47881 tcctgagttt tacctgcgtc atcgcacggg tgatctcatg gctcgtgcga ccaatgacgt
    47941 cgatcgcgtc gtgtttgccg ccggagaagg ggtgctgacg ctggtggatt cactggtgat
    48001 gggctgcgct gtgttgatta tgatgtctac gcaaattagc tggcagttga ccttatttgc
    48061 cctgttgccg atgccagtga tggcgatcat gatcaagcgc aacggcgatg ccttgcatga
    48121 acgctttaag ctggcacagg cggcgttttc tagtcttaat gaccgcaccc aggaaagcct
    48181 caccagtatc cgcatgatca aagcctttgg tctggaagat cgccagtcgg cgttatttgc
    48241 cgcggatgcc gaagataccg gcaaaaaaaa catgcgggtg gcgcgtattg atgctcgttt
    48301 cgacccaacc atctatatcg cgattggtat ggcgaacttg ctggcgattg gcggcggtag
    48361 ttggatggtg gcgcagggca gtttaacgct gggccagctc accagtttta tgatgtattt
    48421 aggtctgatg atttggccaa tgctggcgct ggcatggatg tttaacattg tggaacgtgg
    48481 tagtgctgcg tacagccgta ttcgtgcgat gctggcggaa gcgccggtgg tgaacgatgg
    48541 tagcgaaccc gtgccggaag ggcgtggcga acttgatgta aatattcacc agttcacgta
    48601 tccgcagact gaccatcctg cgctggaaaa cgtcaatttc gccctgaaac ccggtcagat
    48661 gctgggtatc tgcgggccga ctggttccgg caaaagtacc ctgttgtcgc tcattcagcg
    48721 tcatttcgac gtcagcgagg gggatattcg ctttcatgat attcctctga cgaagttaca
    48781 actcgatagc tggcgtagcc gcctggcggt agttagccag acaccgttcc ttttttccga
    48841 caccgtagcg aacaacatcg cgttgggttg cccgaatgcc acccagcaag agattgagca
    48901 tgtcgcgcgg ttagccagcg tacatgacga tattttgcgt ctaccgcaag gttacgatac
    48961 agaagtgggc gagcgcggcg tgatgctttc cggtgggcaa aaacagcgta tctccattgc
    49021 tcgagcgtta ttagtcaacg cggaaatcct catccttgat gatgcgcttt cggcggtgga
    49081 cggacgcaca gagcaccaga tcctgcataa cctgcgtcag tgggaacagg gaagaacggt
    49141 aatcatcagt gcccatcgcc tttcggcact gacggaagcc agtgaaatta ttgtgatgca
    49201 gcacggacat atcgcccagc gtggcaatca tgatgagctg gcgcaacaaa gcggctggta
    49261 tcgcgatatg tatcgctatc aacaactgga ggcggcgctc gacgacgctc cggaaattcg
    49321 cgaggaggcc gtcgatgcgt agttttagcc aactgtggcc gactctcaag cgtctgttag
    49381 cgtacggttc gccgtggcgt aaaccgctgg ggattgcggt cctgatgatg tgggttgcgg
    49441 cagcggcaga agtcagtggg ccgctgctta tcagctattt tatcgacaat atggtagcga
    49501 aaaataacct gccgttgaaa atggttgcag ggctggctgc ggcgtatgtt gggctacaac
    49561 tgtttgccgc cgggctacat tacgcgcagt cgctgctgtt taatcgggcg gcagtaggcg
    49621 tagtgcaaca gttgcgtacc gacgtgatgg atgccgcatt gcgccagcca ttaagcgagt
    49681 ttgataccca acccgtcggg caggtgattt cccgcgtcac taatgacact gaagtgatcc
    49741 gcgatctcta cgttaccgta gtggcaactg tcctgcgtag cgccgcactg gtgggcgcga
    49801 tgctggtggc gatgttcagc ctcgactggc gaatggcgct ggtggcgata atgattttcc
    49861 cggtggtgct ggtggtaatg gtgatatacc agcgttacag cacgccgatt gtccgccgtg
    49921 tgcgcgccta tctagcggat atcaacgacg gctttaacga aatcatcaat ggcatgagcg
    49981 ttatccagca gttccgtcag caggcgcgat ttggcgaacg tatgggggag gccagtcgtt
    50041 cacactatat ggccaggatg caaaccctgc gcctcgacgg ttttctgctg cgtccgctgc
    50101 tgagtctgtt ttcatcgctc attctttgtg gcttgttgat gctgtttggc ttctccgcta
    50161 gcggcaccat tgaagtgggc gtgctgtatg cgtttatcag ctatcttggg cgacttaacg
    50221 aaccattaat cgaactgacc acgcaacagg cgatgctgca acaggctgtt gttgctggtg
    50281 agcgcgtgtt tgaactgatg gacggaccgc gccagcaata tggcaatgat gagcgcccgt
    50341 tacagagtgg caccatcgaa gtcgataacg tgtcatttgc ttatcgcgat gacaatctgg
    50401 tgctaaagaa cattaatctc tctgtgcctt cgcgtaactt tgtggcactg gtcgggcata
    50461 ccggcagtgg caaaagcacc ctcgccagtt tattgatggg ctattacccg ctaacggaag
    50521 gtgagattcg ccttgatggt cgtccattaa gttcgctaag tcacagcgcg ctgcgccagg
    50581 gcgtggcaat ggtgcagcaa gatccggttg tgctggcgga taccttcctc gccaacgtga
    50641 cgctggggcg ggatatctcc gaagaacgcg tctggcaggc gctggaaact gtgcaactgg
    50701 cggagctggc gcgtagcatg agcgacggta tttacacgcc gctgggcgag caggggaata
    50761 atctctcagt tgggcaaaag caactgctgg cactggcgcg cgtgctggtc gagacgccgc
    50821 aaatcctgat ccttgatgag gcaaccgcca gcattgactc cgggactgaa caggcgattc
    50881 aacatgctct ggcggcggtg cgtgaacata ccacgctggt ggtgattgct caccgcttat
    50941 cgaccattgt tgatgccgac accattctgg tgcttcatcg tgggcaagcc gtggagcagg
    51001 gcactcacca gcaactgctg gcggcccagg gacgctactg gcagatgtat caactgcaac
    51061 ttgcgggcga agagctggca gccagcgtgc gtgaagaaga atcattgagc gcctgaatag
    51121 cgcaatattt catcgttggt gcaaaaatgt aacgcactgt gcactgtcat agtgcgtttt
    51181 cattttcaat cttcttaact tcctgctctc tttctcgttt ttcatttctg gcacaccgct
    51241 tgcaatacct tcttcgtgta gcagaaccat taccgaattc tgaccggagg ggatctatga
    51301 agctggtgac cgtgataatc aaaccattca agctggaaga cgttcgtgaa gcgttatctt
    51361 ccattggtat tcagggcctg accgtcaccg aagtgaaagg tttcgggcgt cagaaagggc
    51421 atgccgagct gtaccggggg gcggaataca gcgtcaactt cctgccaaaa gtaaaaattg
    51481 atgtggcgat tgctgatgac caactcgatg aagtgatcga tatcgtcagt aaggcggctt
    51541 acaccggaaa aattggcgac ggcaaaatct tcgtcgctga attgcaacgc gtcattcgta
    51601 ttcgtaccgg cgaagccgac gaagcggcgc tgtaatctct ggcacacagc aacaggaacg
    51661 aaaaatgaag atagcgacga taaaaactgg gcttgcttca ctggcgatgc tgccgggact
    51721 ggtaatggct gcacctgcgg tggccgataa agccgacaat gcgtttatga tgatttgtac
    51781 tgcgctggtg ctgtttatga ctattccggg gattgccctg ttttacggtg ggttgattcg
    51841 cggcaaaaac gtgctgtcga tgctgacgca ggtgacggtg acgtttgcac tggtctgtat
    51901 tctctgggtg gtttacggtt actcgctggc gtttggtgag ggcaacaact tcttcggcaa
    51961 cattaactgg ttgatgctaa aaaacatcga actgacggcg gtgatgggca gcatttatca
    52021 gtatatccac gtggcgtttc agggctcgtt tgcctgcatt accgtcggct tgatcgttgg
    52081 ggcgctggcg gaacgaatcc gcttctcagc tgtgttgatt ttcgtggtgg tatggctgac
    52141 gctctcttac attccgattg cgcatatggt gtggggcggt ggtttgctgg cttctcacgg
    52201 tgcgctggat ttcgcgggtg gcacggtggt gcatattaac gccgcaatcg ccggtctggt
    52261 gggcgcgtat ctgataggca aacgcgtggg cttcggcaaa gaggcgttta aaccgcacaa
    52321 cctgccaatg gtcttcaccg gtactgccat tctctatatt ggttggtttg gctttaacgc
    52381 cgggtcagcg ggcacggcga atgaaatcgc ggcactggca tttgtgaata ctgtggtcgc
    52441 aacggcggcg gcaattcttg gctggatctt cggtgaatgg gcgctgcgtg gtaagccttc
    52501 tctgctggga gcgtgttctg gcgcgattgc cggtctggtc ggcgtgacgc cagcgtgtgg
    52561 ctacattggg gttggcggcg cgttgattat cggcgtggta gctggtctgg cgggcttgtg
    52621 gggcgttact atgctcaaac gcttgctgcg ggtggatgat ccctgcgatg tcttcggtgt
    52681 gcacggcgtt tgtggcattg tcggctgtat catgactggg atttttgccg ccagttcgct
    52741 gggtggcgtg ggcttcgctg aaggtgtgac gatgggccat cagttgctgg tacagctgga
    52801 aagcatcgcc attacgatcg tctggtccgg tgtggtggca tttattggct acaaactggc
    52861 ggatctgacg gttggtctgc gtgtaccgga agagcaggag cgagaagggc tggatgtcaa
    52921 cagtcacggc gagaatgcct ataacgcgta acaagcactg caaaaaatag ccggacggtt
    52981 ttacctccgg ctattttttt aattgtgatt acgcatcacc ccttcctgaa cggtcgaggc
    53041 aaccagcacg ccgtcctggg tataaaactc accgcgcaca aagccgcgtg cgctggacgc
    53101 cgaggtgctc tccacgctat acagcagcca ttcattcaaa ttaaacgggc gatggaacca
    53161 catggaatgg tcaatggtgg caatctgaat ccccggttcg agaaaaccga tgccgtgcgg
    53221 ctgtagagct accggcagga agttaagatc agaagcgtaa ccgagcagat actgatgaac
    53281 gcgcaggtca tccggcacgc taccatttgc gcggatccac acctgacgat gtggttctgc
    53341 gacgtgacct ttcagtgggt tatgaaactc caccggacgg acttccagcg gacgatcgca
    53401 gatgaattta tctttaagca ctggcggcag caggtgcgcc agcgattggg cgatttgcgt
    53461 ttccgaaggc aggccatcag gcgctggcgc ggacggcatt gttttttgat gttcgaaacc
    53521 cgcttctggt gcctggaaag aggccgtcat ataaaaaatc ggtttgccgt tttgaatagc
    53581 agcaacccgg cgggcgctga agctgttacc gtcacgcagc gtttcgacat cataaataat
    53641 cggcttctta ctatcgccag ggcgaagaaa gtagctgtga aacgaatgta ccagccgctc
    53701 ttcagggacg gtctcttttg cagcatacaa ggcctgaccc acgacctggc cgccaaacac
    53761 ctggcgtaaa cctaaatctt cactctggcc gcgaaagagt ccttcctcaa ttttttccag
    53821 atttaacaat gtcagtaaat tttttagcgc ctgactcata taactctcca gtaacaaagc
    53881 tgccgcagca agccaaagtg agttgagtat aacgcaaatt tgctactggt ccgatgggtg
    53941 caatggtctg aattacgggc taattacagg cagaaatgcg tgatgtgtgc cacacttgtt
    54001 gacgttacta ttttgttaac cactcttccg gcgaggaaag ttagcccgct ggtgcattga
    54061 taataaggag aaatgaatga aactcgtgca catggccagt ggtttagcgg ttgcgattgc
    54121 gttggcggct tgcgcagata aaagcgcgga tattcagacg ccagccccgg ctgcaaatac
    54181 gtctatttca gcaacacaac aacctgctat ccagcaaccg aatgtctccg gtaccgtctg
    54241 gatccgtcag aaagtcgcac tgccgcctga tgctgtgctg accgtgacac tttctgacgc
    54301 gtcgttagcc gatgcaccgt caaaagtgct ggcgcagaaa gcggtgcgta ctgaaggtaa
    54361 acagtcacca ttcagctttg ttctgccatt taacccggca gatgttcagc cgaacgcgcg
    54421 tattctgttg agtgcggcga ttaccgtgaa tgacaaactg gtatttatta ccgataccgt
    54481 tcagccggtg atcaacaagg gcggaactaa agccgacctg acattggtgc cggtgcagca
    54541 aaccgccgtg ccggttcagg ccagcggtgg tgcaacgact accgtacctt cgacttcacc
    54601 aactcaggtg aatccgtctt cggcagttcc agttcctacg caatattaag cgacgttaac
    54661 cctctccgcc cggagagggt tagtagttcc agcgataacg ctgcaagtcg atttgcccgc
    54721 ttcccgatac catcacacct tctgccagta atgcctgtcg ctgacgctgt aaatccggtc
    54781 cggttagcga aattgtgccg tggcgattaa ccacccggtg ccagggtaag gtgctgcctt
    54841 cagggagacg ctttaacaca ccgcccacct ggcgcgcggc gcggggcgat cccgccagtt
    54901 tcgccacatc accgtaagtg gtgacatagc cttcgggaat agcggcgacg atttgccaga
    54961 cgcgttgggg aaatgaatct tctttttcca tcttttcttc ctgaggtaat ttttcagcat
    55021 aatctggaaa aacgcccgag tgaagtcgca ttgcgcaaga aaccagcatc tggcacgcga
    55081 tgggttgcaa ttagccgggg cagcagtgat aatgcgcctg cgcgttggtt ctcaacgctc
    55141 tcaatggggg ctctgttggt tctcccgcaa cgctactctg tttaccaggt caggtccgga
    55201 aggaagcagc caaggcagat gacgcgtgtg ccgggatgta gctggcaggg cccccaccca
    55261 tttctgcctc ccaccgtttc gtcaaaaaac cccaacatgg ctaaacttta accacaactg
    55321 acgtcgcaag aattgtctgg ctgcgcagta cgcttcggag gtatgtctga tgaagtatgt
    55381 tgatggtttt gtggttgccg ttcctgccga taaaaaggat gcctatcggg aaatggccgc
    55441 taaggctgcg ccattgttta aagagtttgg cgcgcttcgt attgtcgaat gctgggccag
    55501 cgatgtaccg gatggcaaag tgaccgattt tcgtatggcg gtgaaagcgg aagagaatga
    55561 agaggttgtc tttagctgga ttgaataccc ttcaaaagag gtccgcgacg ctgctactca
    55621 aaagatgatg tcggacccac ggatgaaaga gttcggcgag tccatgccgt ttgacggcaa
    55681 gcgaatgatc tatggcggat tcgagtcaat catcgacgaa taggtagcgt gacgggctgt
    55741 gcggcgtgcc gtcagcccaa aaattcacaa atgttgctcg gcccagcgta agaaatcttc
    55801 tttcggtaat gccttgctgt agagccagcc ctgaccgtag tgcacgccat gctggcgtag
    55861 ccactcttct tgtttactgg tttcgattcc ctccgctacc attttcagtt tcagtgtttt
    55921 tgccatttcg atgatatgcg gcgtgacatt tttatattcc agcgcatcaa cgaaagattt
    55981 atcgatcttc agaatgtcga catccagatc ctgtaaataa cttaaacttg aatatcccgt
    56041 accaaaatca tcaagataaa tttcatggcc cgcctcccgg tagcgagaaa ttatcggggc
    56101 gctggttttc ggatcggcaa actcgcgttc agtgagttca agcgcgatct gtctgggatt
    56161 aacctgatag tgattgatca tttcacgcag caattgcggg attttttccg aggtgagcac
    56221 ggtggattca agattgatcg aaatatgctg ctgtggatgc tgacgcagcc agtcgcccat
    56281 atcttcaaag acgcttctta taatcagtag cgtcaatggc tcagaaaggc ccgtttgctg
    56341 tgccagcgga ataaaactat ctggtgacaa ccaactaccg tctgtctgcg gccagcgcgc
    56401 cagtgcctca gcaccgacaa ttttgccatt ggctaaggag acaatcggct gatagtgcac
    56461 gcaaatatca cgattttcga tagcatcctg cagccgatga tgcggtgact ggatacggcg
    56521 caaaatacgc agcacaaaca tcgctgccag caggccaatc accaacccga gcggtaacca
    56581 aataaagact tgccgatgcc agcctttctg caacattttc gttgaagccc aggtgatgat
    56641 cgaaatattc atctccggga agggcaggat atcgtaaatg attccattat tttcgatatg
    56701 ctcaccgggt gttttttgta gcctggtaat aattccctga gcaatttcat cgctgctggt
    56761 tatgacaacg ttatgggcat tgccaataat ggcggcatca atttgccatg agctataggg
    56821 aatgacatca ataaaggaag cggggtcgat catgacgaca taatgtgccg ttcccatggc
    56881 gaccatgtaa cggataatgc ctaaatcgtt atgcgatgtt aaccagacac gataaccatc
    56941 tttcgaaatt ttacctggct cggggaaggt atcgggcgga ctttcatgct ccagagacga
    57001 acactgggga acgttgttat cgatataaac cacttcctga atatagcgat aactgtaaga
    57061 tacccgacgc atttccatga gatgggcttc gctacaggca gcgccttgcc atctttccag
    57121 ctcttgcagc gcatctttcc cttgtgtcgc caccttattg gcccgaatag cgacgcggga
    57181 ggaataggta tccagctctt caataaacga tgtttctacc tgctgatggg ccagccagat
    57241 gcttaagccg acaggcagca atactgaaag aatcagtact cccgaaataa ggccgaccag
    57301 atgtcgtgtt ctcaccagca tatccttatt aacacagtgt agggaaagta tatctgattg
    57361 tggaaaaagg ctggcaggag ataaggaggt agagctggta tttcatgaag tgatgggtgg
    57421 aaacgatcat tgtcatttga ccaggcgcag agctttacgc ctggtatgta accctaactt
    57481 aagatgttga ttcggcgcga gcgagggtaa aaatatcgta aaaagacagt tcacctttac
    57541 ggacgatcat tttttgcagt tgttccttat gaacatccgc gacacgcgga gagttcaaga
    57601 tggcttcaac cagcgcggca ggtacaaagc gcgtgttgta ccattcgccg ttatagcaaa
    57661 cgcggaaatc cagcacgtcg atatcttcat agcggtagcg tgggtagaca tcgaaaaaga
    57721 agaaaccatt aagcaggata aataatacaa ccagtagcca gacagagcct gacagcgttt
    57781 cggactgcaa catcaccgcc agggtggcaa aaaaagcaac gtacataccg ataaacagcc
    57841 ccggatgttt acggataaaa ctgatactaa agcggggttt gttgtcgcgt ttttcgcggg
    57901 tattcagaga ctcaatcgtt tcggtcagca ggcgttgtat ttcggtcatc gtttgccttc
    57961 gtggtatctg caactttcca gggaacaccc tattttaacg tgggaatagt agaggggaag
    58021 taacagattt accacaattt tgcataacag ttgcgaaaaa ccgattacaa ttttttaatt
    58081 attctggctg gattaccgcc cacgacaacg ttgtccggga catcttttgt gacaactgca
    58141 cctgaggcta ccacgacgtt atcaccaatg gtcacaccag ggttaatgac cgcgcgtccg
    58201 ccaatccaga cgttattacc gatggtgacg ggtttcccca gttcagcacc gctattacgt
    58261 gctacagggt cgatgggatg tgttgccgtg tagatatgaa cgcctggtgc caacatacag
    58321 ttatcaccga tgcgaatagg gcagacatca agcatcacgc aatcgaagtt ggcgaaaaaa
    58381 ttattaccga gaaaaatgtt atagccatag tcacagcgaa acgttggctc aatataagcc
    58441 tctgtcacct gaccgaatag atcagcgaga atttgctggc gtaatgtgtg ctcttccgcc
    58501 agggaatgat tgtatcggtg aataagctga cgagcgcgca ggcgatcgcg agataacgtc
    58561 tcatctgccg agcgatacaa ctcaccagca atcatctttt ctttttctgt gctcattact
    58621 gacctccggt tgctgaaaat gcggcaacac agtaagacga aaagtcagtg aagagaacgt
    58681 tcccattaca tctgtgtgat aaagatcaca tagggtatgt cttcatggtg aaaaagtata
    58741 aaattcttaa taaacagccg gttatagctc cgaaagcgaa ttatttatta gcgaataaat
    58801 ttccatactg aggaagggat cttgtcgtat agtttattca tggtcaattc ggcgaggcgg
    58861 tgatctgcgg ctgagtaaaa taccgccagt tcattatctg ataattcgta tttatttttc
    58921 tcgataacac gctccagcgt gtcaattgtc tggcaacgac gtaaacgcat taaataatcg
    58981 gttttcgtta aaggtttttc ggacatactt ctacccatgg ttgtaataat tctaacaaga
    59041 taaactcgca ggattctctt tcgtcgcatt gacaaaacaa cggaatagtc gattacctga
    59101 cttccgccat ttctgaagat cctgcatatt aataccataa ctgctgaaca acataaaggt
    59161 gtcatccaga tattcgtcga tctgctcaat gagcttattg tcttcattat acttaatttt
    59221 gtaattaagt gcgaaggtcg caatatgctc aatcagttca ttcaactgga ggttgatcgc
    59281 cgaggttggg tcgttaaccc agccatgatt gctttcttca aggtttgcaa gacagtcatg
    59341 atacagggtt tcacagagaa acttaagctg tgcgatatca tgtcttttgg gtgagtattc
    59401 atccataacg cgtccccttc ttagcggttg aactaacgga cacctttcgg gatggaaaaa
    59461 acttactgac ctggacttgc cctctcgttt gttagcttaa ctataagcca ctctttgcag
    59521 gttttcatcg cattattacg aaaaattaca attaggaagg aagcgggaca ggactcactt
    59581 tttcatacta gcactaaccc gcagcaggta aaagcagttt gctcgaacta ctcataacat
    59641 ttcaggatta tcgccagatg aaattggtga cactaatacc aggattgctc tgaatatgac
    59701 gtaataaccg aggaatgaat aaagaattac cgcaaataat taagaatagc ctctaaatga
    59761 ttatggatta ttaaatctat tagcgctact taatataaat taactaacaa tcagtatttt
    59821 ttatgaattt tatacgtggt taatactggt tttcgtatga gatcctgagt tggtggttca
    59881 attactcctt aatgttcgta ggttatgcat aaaaaaggcc gcttacgcgg ccttagtgat
    59941 tacatgttgt atcaatgatg atcgacagta tggctgtgct cgatatcttc attcttgcgg
    60001 ctaaagcggc ggcgaaccac cacaaagaat accggaacga agaagattgc cagtaccgtt
    60061 gcggtcacca tcccgcccat tacaccggta cctactgcgt tctgcgcgcc ggaaccagca
    60121 ccagtactga taaccagcgg cataacgccg aggataaacg ccagcgaggt catcaggatc
    60181 ggacgtaaac gcatccgcac cgcatcaagc gtcgcttcaa tcagaccttt accttcttta
    60241 tccatcaagt ctttggcgaa ttcgacgata agtatcgcgt tcttcgccga caacccaatg
    60301 gttgtgagca ggcctacctg gaagtaaacg tcattggtca ggccacggaa ggtggcagcc
    60361 agcaacgcac cgataacccc cagcggaacg accagcataa cggagaacgg aatcgaccag
    60421 ctctcgtaca gtgctgccag acacaggaac acgacaatca acgaaatcgc gtacagtgaa
    60481 ggtgcctggt tgccggagag acgttcctga taggacatcc ccgtccagtc atagcccaca
    60541 ccggtaggca gtttgctcgc cagttgttcc attagctcca ttgcttcacc ggtactttta
    60601 cccggtgccg cctggcctaa gatttccata gatggcaggc cgttgtaacg ttccagacgc
    60661 ggcgaaccgt actcccaacg agaagaggag aacgccgaga acggcaccat ctgaccatca
    60721 gcagcacgaa cataccagtc gccgatatca tccggcagca tacggtattt cgcttctgac
    60781 atgacgtaaa ctttcttcac acgaccgcgg tcgataaagt cgttcacata gctaccaccc
    60841 catgcagcgc ccagagtggt gttaatgtcg ttgatagaaa cacccagcgc ctgcgctttt
    60901 tcctggtcga tatcaatctt aaactgcggg gtatcttcca gaccgtttgg acgtacgctg
    60961 gtcaacatat cagggtgctt cgctgcttct gcaagcagct ggttacgcgc ctgagtcagt
    61021 ttttcgtgac caaggccagc ctggtcaatc agctcaaagt caaagccggt tgcagtaccc
    61081 agttccacga ttgcgggcag gttaaaggcg aaaaccatcg catctttgat ttgcgagaaa
    61141 gcgcgtgttg cacgcatagt aatcgcttca actttgtttt cttcgcccgg acgatcggcc
    61201 cagtccttca aggaaacgaa cgcaataccg gtattctgac cacgtcccgc aaagccgaag
    61261 ccgttaacgg cgaacaccga ctcaacgttg ttcttttctt tggtcagata gtaatgcgtt
    61321 acctcattga gcactttctg cgtacgttcc tgcgttgcac ctgctggcag ctgaaccatg
    61381 gtcataaata cgccctggtc ctcatctggc aagaaggagc ttggcagacg cacgaacaga
    61441 taggccatgc cgaccacgat gatcagatac agcaccaggt aacgccccgt actgcgcaga
    61501 ataccgccta cgctgtcggt gtagtggtgc gtgctcttct cgaacatgcg gttaaaccag
    61561 ccgaagaagc cttttttacc ttccccgtga tcgcctttgg caatcggttt cagcatggtg
    61621 gcacaaagag ccggagtcag gatcaacgct accagtaccg acagcgccat tgctgaaaca
    61681 atggtaatag agaactgacg atagatagca ccagtagaac cgccaaagaa ggccatcggt
    61741 acgaataccg ccgacagtac catcgcgata ccgaccagag cgccctgaat ctgccccatc
    61801 gacttacggg tagcttcttt tggcggcaaa ccttcttccg ccataacacg ctcaacgttt
    61861 tctaccacaa cgatagcgtc atccaccaac aagccgatgg cgagcaccat cccgaacatt
    61921 gttagcgtgt ttatcgagaa gccaaaggcg gcaaggacgg caaaggtccc gagcaatacc
    61981 accggtacgg caatggtcgg aatcaacgtc gcgcggaagt tctgcaggaa cagatacatc
    62041 accaggaaca cgaggatgat cgcttcgacc agcgttttaa ccacttcgtg aatagagatt
    62101 ttcacgaacg gcgtggtgtc gtacgggtaa acaattttca gacctgacgg gaagaacggt
    62161 tccatcttcg ccagttcagc acggattgcc gcagcggtat ccagcgcgtt tgcaccggtc
    62221 gccagcttga tccccagacc ggaagccggt tggccgttaa actctgcgat gatgtcgtag
    62281 ttctcaccac ccagctcaat cttcgccaca tcacgcagca gtacgcggga accatcctga
    62341 ttcactttca gcaggatttt gccgaactct tcagtagagg tcagacgcgt ctgagcaata
    62401 atagaggcgt taagctgttg gcctttcacc ggcggcgtac caccgagctg acccgccgca
    62461 acctgggcgt tctgcgcttt gatggcggta atgacatcaa ccggcgttag ctggaatttg
    62521 ttcagctcat tcgggttcat ccagatacgc atcgcgtact gtgaaccgaa caactgaaca
    62581 tcacccacgc ccgacgtacg gctgatggca tctttcatat tcgccgctac gtagtcagag
    62641 atatcctcct gcgtcatggt gccatcggtg ttgataacgc cgacaaccat caggaagctg
    62701 ctggatgatt tctcaacgct caccccttgc tgctgaactt cttgcggcag caacggcatc
    62761 gccagctgca gtttgttctg cacctgaacc tgcgcgatat ccgcatcagt accagactca
    62821 aaggtcaggg tgatctgcac ggtacccgtg gagtcactgt tagaggacat gtacatcagg
    62881 ttatcgatac cgttcatatt ctgttcgata acctgtgtca ccgtgtcctg cactgttttc
    62941 gcatcagcgc cggggtagga ggcggagatc gttactgccg gcggtgcaat cgtaggatat
    63001 tgcgccaccg gcagtttgag gatcgccagc ccccctgcca acatgatgat aatggcgatc
    63061 acccacgcaa aaatcgggcg atcgataaag aaattaggca tgtcttaacg gctcctgttt
    63121 aagttaagac ttggactgtt caggctgagc accgcttgcg gcttgctggt tattatcagc
    63181 ggtaacttct tgtgctttta cctggacacc aggacgcact ttctgcagcc cacttattac
    63241 tacgcgatcg cctgctttca gaccttctgt caccagccac ttatcgccaa tagcctggct
    63301 tgcaacgatc ggacgggtct ccactttgtc atccgcgcca actaccagta cggtggcatc
    63361 gccacgcggc gtacgggtta cgccctgttg cgggactaaa atagcgtttg gattaagccc
    63421 ttcttcaaga cgtgcgcgca cgaacatacc cggcagcaga gtgtgatccg ggttcgggaa
    63481 gatagcgcgt agggtgatag acccagtggt ctgatcaacg gtaacgtcag agaattccag
    63541 cgtaccgtcc tgcgggaact taatgccgtc actggtgatc agcgacactt tggctttgcc
    63601 gttctcttgt ttcagcgtgc cattcgccag ttcctgtttc aggcgcagga agtcgttgct
    63661 ggactgggtc acatcaacgt agatcggatc aagttgctgc acggttgcca gcgcagtcgc
    63721 ctgaccgttc tgtaccaatg cgccttccgt cacgttcgac ttaccaatgc gaccgctaat
    63781 cggagaggtg actttggtgt aagccagatt gatccgcgca gtttcaacgg cagctttcgc
    63841 cgcagttacc gcagcattcg cctgttgcgc atcagccaga gcctgatcgt actcttgctt
    63901 actgatgtac tgagtaccga gcagtttctg ataacgattc accgtcaatt gcgcgatatt
    63961 ggctgcagcc tgggctttcg ccagatcacc tttcgcactg tcgtatgtcg cctgataggt
    64021 cgcaggatca atctgataga gagagacacc tgcttcgatg tcgctacctt ctttgaaatt
    64081 acgcttcagg ataatcccgc taacttgagg acgaacttct gcgatccggt aggcactggt
    64141 gcgacccgga agctcggttg tgatctgcag aggttcagtt ttgactgtta ctacgccaac
    64201 ggcgggcatc tgctggccac cttgttgggc ctgtttgtcg tcacatcctg ttagggctaa
    64261 gctgcctgag agcatcagaa cgaccgccag aggcgtaaac cctctgtttt tgttcatatg
    64321 taaacctcga gtgtccgatt tcaaattggt caatggtcaa aagttaataa acccattgct
    64381 gcgtttatat tatcgtcgtg ctatggtaca tacattcaca aatgtatgta aatctaacgc
    64441 ctgtaaattc acgaacatat ggcacgaaaa accaaacaag aagcgcaaga aacgcgccaa
    64501 cacatcctcg atgtggctct acgtcttttc tcacagcagg gggtatcatc cacctcgctg
    64561 ggcgagattg caaaagcagc tggcgttacg cgcggtgcaa tctactggca ttttaaagac
    64621 aagtcggatt tgttcagtga gatctgggaa ctgtcagaat ccaatattgg tgaactagag
    64681 cttgagtatc aggcaaaatt ccctggcgat ccactctcag tattaagaga gatattaatt
    64741 catgttcttg aatccacggt gacagaagaa cgacgtcgat tattgatgga gattatattc
    64801 cacaaatgcg aatttgtcgg agaaatggct gttgtgcaac aggcacaacg taatctctgt
    64861 ctggaaagtt atgaccgtat agaacaaacg ttaaaacatt gtattgaagc gaaaatgttg
    64921 cctgcggatt taatgacgcg tcgggcagca attattatgc gcggctatat ttccggcctg
    64981 atggaaaact ggctctttgc cccgcaatct tttgatctta aaaaagaagc ccgcgattac
    65041 gtcgccatct tactggagat gtatctcctg tgccccacgc ttcgtaatcc tgccactaac
    65101 gaataaccct gaatctgact ccaggatttt tcctggacat tttcgtcgtt gctattctgg
    65161 ttcactgcgt cgtgatattc ttgcggtttg actttttcag gtcgttcttc aggttcagaa
    65221 accttcattc atcatgacta tgttccagta ttacaaacga tcacggcatt ttgttttttc
    65281 agcatttatt gcttttgttt ttgtcttgtt atgccagaac acggcgtttg cgcgggcgtc
    65341 atcgaatggt gatctgccga caaaagcgga cctgcaggcg caacttgact cactaaataa
    65401 acaaaaagat ctttctgctc aggacaaact ggtgcagcag gatctgacag atacattagc
    65461 caccctcgat aaaatcgatc gcataaaaga agagacagtt cagctacggc aaaaagtcgc
    65521 tgaagcgccg gaaaaaatgc gccaggcgac cgcggcgtta acagcactta gcgatgtcga
    65581 taacgacgaa gaaacgcgca aaattctgag tacgctttcg ttgcgccagc tggaaactcg
    65641 cgttgcccag gcgctggacg atttgcaaaa cgcacaaaac gatctggcgt cttataacag
    65701 ccagctggtt tcgttacaga cgcagcccga acgcgtgcaa aatgcgatgt ataacgcttc
    65761 gcagcagctg caacaaattc gcagtcgtct ggatgggact gatgtcggcg agactgcctt
    65821 acgtcccagc cagaaagtgt taatgcaggc tcagcaggcg ttgctgaatg cggagattga
    65881 ccagcagcgt aaaagcctgg aagggaacac cgtcttgcag gataccttgc aaaagcaacg
    65941 tgattacgtg acggcgaaca gcgcccgtct ggagcaccag ttgcagctgt tgcaagaagc
    66001 ggtaaacagc aagcgcctga ctttaactga aaaaacggcg caggaagccg tctccccgga
    66061 tgaagccgcg cgtattcagg ctaatccgct ggtgaaacag gaactggaaa ttaaccagca
    66121 gttaagtcag cgtctgatta ccgcgactga aaacggtaat cagttgatgc agcaaaacat
    66181 taaagtcaaa aactggctgg agcgggcgct gcaatcggaa cgcaatatta aagagcagat
    66241 tgccgtcctg aagggcagcc tgctgttgtc tcgtatcctt taccagcaac aacaaacgct
    66301 gccctcggcg gatgaactgg aaaacatgac caaccgcatc gcggatttgc gtctcgaaca
    66361 gtttgaagtt aaccagcagc gtgatgcact tttccagagc gatgcgttcg tcaacaaact
    66421 ggaagaaggt cacaccaacg aagtcaacag cgaagttcac gatgcgttat tgcaagtggt
    66481 tgatatgcgt cgcgaattgc tggatcaact caacaaacag ttgggtaacc agctgatgat
    66541 ggccattaac ctgcaaatca accagcagca gttaatgagt gtgtcgaaaa acctgaaatc
    66601 catcctgact cagcaaatct tttgggtgaa cagtaaccgt ccaatggact gggactggat
    66661 caaagcgttc ccgcaaagcc tgaaagatga atttaagtcg atgaaaatca cggtgaactg
    66721 ggaaaaagcc tggcctgccg tttttatcgc tttcctcgcc ggtttgccgc tgctgttgat
    66781 tgccgggctg atccactggc gtctgggctg gctgaaagcg tatcaacaga aactggcttc
    66841 cgctgtgggt tccctgcgta acgatagcca gctcaacaca ccaaaagcga tccttatcga
    66901 cctgatccgc gcgctgccgg tgtgcctgat tattctcgcg gttggcctga ttctgttgac
    66961 catgcagctc aacatcagcg aactgctatg gtcgttcagt aaaaaactgg cgattttctg
    67021 gctggtgttt ggcctgtgct ggaaggtact ggagaaaaat ggcgttgccg tgcgtcactt
    67081 cggcatgccg gagcagcaga ccagccactg gtgtcggcaa attgtccgca tcagtctcgc
    67141 gttgctgcct atccatttct ggtctgtggt agcagaactt tccccgctgc atctgatgga
    67201 tgatgtgctg gggcaggcga tgattttctt caacctgctg ctgattgcct tcctggtatg
    67261 gccaatgtgc cgcgaaagct ggcgtgataa agagtcgcac accatgcgac tggtcaccat
    67321 taccgtgcta tcgattatcc cgattgcgtt gatggtgctg actgccacgg gttatttcta
    67381 caccacgctg cgcctctccg ggcgttggat tgaaaccgtt tatttggtga tcatctggaa
    67441 cctgctgtac cagacggtat tgcgcggctt aagcgtagcg gcgcggcgta tcgcctggcg
    67501 tcgtgcgctg gcgcgtcggc agaatctggt gaaagagggc gcagaaggtg ccgaaccgcc
    67561 ggaagaaccc accattgcac tggagcaagt taaccagcag acgctgcgta ttaccatgtt
    67621 gctgatgttt gcgctgttcg gtgtcatgtt ctgggcaatt tggtccgatt tgatcaccgt
    67681 gttcagctat ctcgacagca tcacgctctg gcattacaac ggcactgaag ctggcgctgc
    67741 ggtggtgaaa aacgtcacca tgggcagtct gttgtttgcg attatcgcct caatggtggc
    67801 ctgggcgttg attcgcaacc tgcctggttt actggaagtg ctggtgctct cgcgactgaa
    67861 tatgcgtcag ggcgcgtcgt atgccattac taccatcctt aactacatca ttattgccgt
    67921 tggtgcgatg acggtgttcg gatcgctggg cgtctcttgg gataaactcc agtggctggc
    67981 cgcagcatta tccgtaggtc ttggttttgg tttacaagaa attttcggta acttcgtctc
    68041 cggtttgatc attctgttcg aacgtccggt gcgtattggc gatacggtaa ccattggtag
    68101 cttctcgggg acggtaagta agatccgtat tcgtgcgaca acgattaccg atttcgatcg
    68161 caaagaagtg atcatcccga acaaagcgtt tgttaccgag cgtctgatca actggtcgtt
    68221 gactgacact actacgcgtc tggtgatccg tctcggcgtg gcctatggct ccgatctgga
    68281 aaaagtgcgt aaagtgttac tgaaggcggc gactgagcac ccaagggtga tgcacgaacc
    68341 aatgccggaa gtcttcttta cggcgtttgg tgccagcacg ttggatcatg agctgcgtct
    68401 gtatgtgcgt gaactgcgtg accgtagtcg tactgtcgat gagctgaacc gtactatcga
    68461 tcagctgtgc cgtgaaaacg acatcaacat tgcctttaac cagcttgaag tgcatctgca
    68521 caacgagaag ggcgatgagg tgacggaagt caaacgcgac tacaaaggcg atgacccgac
    68581 gccagcggta gggtaataac caaaggtggc gacatttagt tgccccgaga ttactgacaa
    68641 agtgcgcgtt gttcatgccg gatgcggcgt gaccgcctta tccggcctac gaaaccgcaa
    68701 gaattcaatg tattgcagga gcggtgtagg cctgataagc gtagcgcatc aggcagtttt
    68761 gcgtttgccc acaatcttag gggacattta gcggccccat ttatttctca cttttccgcc
    68821 tcatcatcgc gcgttaattt cttttcataa tcgcgtttta caatatccag cgcgcgcagc
    68881 acggtgcggg ctgggatctg attttcttcc agcagcacaa tcaaatcgac agccagtttg
    68941 acatcgtcag gggcattttc cagtgacata ctctctccat tgctagcggg ttaagcgcgc
    69001 tagcctgttt tcgatttttt ccagcgcatg gcggcagcgt gccaggcgcg cttcataggc
    69061 ttccacttca cgatgcagcg tttgctgttc cacgagatcg gtcacccgcg ccaggcgggc
    69121 tctgcgttcg gcaaccatct cacgtagccg ccgctcaaaa tcctgatgct gaatacgttt
    69181 acgctgccag cgggcaattt tcggtggcgc actgtcccac tcgcgcaatg accaggcgga
    69241 ggcttcacgc gcgatggctt ccagttgtgc cgccagatgt tccgccagcc aggccacttg
    69301 cggcagctgt tgctgctcaa ctgcatgacg aagcgcagcc agattatcgc ccgcctcatc
    69361 gagacaagcc tgcagtgttg tcgcacgggt ttgaaaaagg tgtctgtcaa aacgggcact
    69421 tagcgtggca aattgcgcta ccggggcaca acgctgacgc agcgtagcga gctgtccttc
    69481 cagtttttcc agcagcaggg cggttttcac gataagacct caatgaaaat gataattgtt
    69541 atgctaaagt agccactctg taagctgaca acactcaata tgcaacgaat cattttaatc
    69601 atcattggct ggctggcggt agtgctgggt acgctgggcg tggtattacc ggtattaccg
    69661 acgacgccgt ttatcctgct ggcggcctgg tgctttgccc gttcttcccc gcgctttcac
    69721 gcttggttgc tgtaccgctc atggtttggc agctatctac gtttctggca gaaacatcat
    69781 gcgatgccgc gcggcgtcaa accgcgggcg attttgctta ttttgctcac gtttgccatt
    69841 tctctgtggt tcgtccagat gccatgggtg cgcatcatgt tgctggtaat tctcgcctgt
    69901 ttgcttttct atatgtggcg aattccggtg attgatgaaa agcaagaaaa gcactgaagc
    69961 acaacaatcg cagttgcaat tattgcgtac agccagtaca ttctggcgtt ttcgagcaca
    70021 ggcgcaggcg gtcaaaggtt aaacaactgt tacttttgat acgtttaaaa cgcgccgtga
    70081 gtaccaccgt aacaagcagg catacactta tgaccgcgac tgcacagcag cttgagtatc
    70141 tcaaaaatag catcaaaagc attcaggact acccaaaacc cggcattctt ttccgcgatg
    70201 tcaccagctt actggaagac ccgaaagctt acgctctcag catcgacttg ctggttgagc
    70261 gttacaaaaa tgcgggcatt accaaagttg tcggcaccga agcgcgtggc ttcttgtttg
    70321 gcgctccggt agctctgggt ctgggcgttg gctttgtacc ggtccgtaaa ccgggcaaac
    70381 tgccgcgtga aaccatcagt gaaacttacg acctggaata cggcaccgat cagctggaga
    70441 tccacgttga tgccatcaaa ccgggcgaca aagttctggt ggtggacgat ctgctggcaa
    70501 ccggcggcac tatcgaagcg accgttaaac tgatccgtcg tctgggtggt gaagtggctg
    70561 acgctgcgtt cattatcaac ctgttcgatc tcggcggcga acagcgtctc gaaaaacagg
    70621 gcattaccag ctacagcctt gtcccgttcc cgggccatta attatcgcca gtcttgtgct
    70681 gcccacgcta cggacagcac aagatgtgca ttcagcctcg ccgttctgac ggggctgtgt
    70741 tagcattacc ccttcgtgaa tccaccttcc agcgtttcag agcctgccaa tgagttatca
    70801 ggtcttagcc cgaaaatggc gcccacaaac ctttgctgac gtcgtcggcc aggaacatgt
    70861 gctgaccgca ctggcgaacg gcttgtcgtt agggcgtatt catcatgctt atcttttttc
    70921 cggtacccgt ggcgtcggaa aaacctctat cgcccgactg ctggcgaagg ggctaaactg
    70981 cgaaaccggc attaccgcga cgccgtgcgg cgtgtgcgat aactgtcgtg aaatcgagca
    71041 ggggcgcttt gtcgatctga ttgaaatcga cgccgcctcg cgcaccaaag ttgaagatac
    71101 ccgcgacctg ctggataacg tccagtacgc tccggcgcgt ggtcgtttca aagtttatct
    71161 gatcgacgaa gtgcatatgc tgtcgcgcca cagctttaac gcactgttaa aaacccttga
    71221 agagccgccg gagcacgtta agtttctgct ggcgacgacc gatccacaga aattgccggt
    71281 gacgattttg tcgcgctgtt tgcaatttca tctcaaggcg ctggatgtcg agcaaattcg
    71341 ccatcaactt gagcacatcc tcaacgaaga acatatcgct cacgagccgc gggcgttgca
    71401 attgctggcg cgcgccgctg aaggcagcct gcgagatgcc ttaagtctga ccgaccaggc
    71461 gattgccagc ggtgacggcc aggtttcaac ccaggcagtc agtgcgatgc tgggtacgct
    71521 tgacgacgat caggcgctgt cgctggttga agcgatggtc gaggccaacg gcgagcgcgt
    71581 aatggcgctg attaatgaag ccgctgcccg tggtatcgag tgggaagcgt tgctggtgga
    71641 aatgctcggc ctgttgcatc gtattgcgat ggtacaactt tcgcctgctg cacttggcaa
    71701 cgacatggcc gccattgagc tgcggatgcg tgaactggcg cgcaccatac cgccgacgga
    71761 tattcagctt tactatcaga cgctgttgat tggtcgcaaa gaattaccgt atgcgccgga
    71821 ccgccgcatg ggcgttgaga tgacgctgct gcgcgcgctg gcgttccatc cacgtatgcc
    71881 tctgcctgag ccagaagtgc cacgccagtc ctttgcgccc gtcgcgccaa cggcagtaat
    71941 gacgccaacc caggtgccgc cgcaaccgca atcagcgccg cagcaggcac cgactgtacc
    72001 gctcccggaa accaccagcc aggtgctggc ggcgcgccag cagttgcagc gcgtgcaggg
    72061 agcaaccaaa gcaaaaaaga gtgaaccggc agccgctacc cgcgcgcggc cggtgaataa
    72121 cgctgcgctg gaaagactgg cttcggtcac cgatcgcgtt caggcgcgtc cggtgccatc
    72181 ggcgctggaa aaagcgccag ccaaaaaaga agcgtatcgc tggaaggcta ccactccagt
    72241 gatgcagcaa aaagaagtgg tcgccacgcc gaaggcgctg aaaaaagcgc tggaacatga
    72301 aaaaacgccg gaactggcgg cgaagctggc ggcagaagcc attgagcgcg acgcgtgggc
    72361 ggcacaggtg agtcaacttt cgctaccaaa actggtcgaa caggtggcct taaatgcctg
    72421 gaaagaggag agcgacaacg cagtatgtct gcatttgcgc tcctctcagc ggcatttgaa
    72481 caaccgcggt gcacagcaaa aactggctga agcgttgagc acgttaaaag gttcaacggt
    72541 tgaactgact atcgttgaag atgataatcc cgcggtgcgt acgccgctgg agtggcgtca
    72601 ggcgatatac gaagaaaaac ttgcgcaggc gcgcgagtcc attattgcgg ataataatat
    72661 tcagaccctg cgtcggttct tcgatgcgga gctggatgaa gaaagtatcc gccccatttg
    72721 atcgtaagca cagcttacgt tcgtcatcct taacgtgatt gagagagaaa cctatgtttg
    72781 gtaaaggcgg tctgggtaac ctgatgaagc aagcccagca gatgcaagaa aaaatgcaga
    72841 aaatgcagga agagatcgcg cagctggaag ttaccggcga atctggcgca ggtctggtaa
    72901 aagtgaccat caacggtgca cacaactgcc gtcgcgtaga gatcgacccg agcctgctgg
    72961 aagacgacaa agagatgctg gaagacctgg tggctgcagc attcaacgac gcagcacgtc
    73021 gtattgaaga aacgcagaaa gaaaaaatgg cctctgtatc ctccggaatg cagctgccgc
    73081 ctggctttaa gatgccgttc tgatgcaaac cagcccgctg ttaacacagc ttatggaagc
    73141 actgcgctgt ctgccgggcg ttggcccgaa gtcggcgcag cgtatggcgt tcacgctgct
    73201 tcagcgcgat cgtagcggcg ggatgcgtct ggcgcaggcg ctcacccggg cgatgtcgga
    73261 aatcggccac tgcgccgatt gccgcacttt caccgaacag gaagtctgta acatctgttc
    73321 gaatccgcgt cgtcaggaaa acggtcaaat ctgtgtggtg gagagtccgg cggacatcta
    73381 cgccattgag cagacggggc agttttcagg ccgttatttt gtgttgatgg ggcacctgtc
    73441 accgctggac ggcatcggtc cggatgatat cggccttgat cgtctggaac agcgtctggc
    73501 agaggaaaaa atcactgaag tgatcctcgc caccaatccc acggttgaag gtgaagctac
    73561 cgctaactac attgccgagc tttgcgcgca atatgacgtg gaagccagcc gaatcgctca
    73621 tggcgtaccg gttggcggcg aactggaaat ggtcgatggc accacgctgt cacactccct
    73681 tgccgggcgt cataagattc gtttttaagc aaacgagagc aggatcacct gctctcgctt
    73741 gaaattattc tcccttgtcc ccatctctcc ctcatcctgt ttttaacctt aaaatggcat
    73801 tattgaggta gacctacatg aaaggacaag aaactcgtgg ttttcagtca gaagtgaaac
    73861 agcttctgca cctgatgatc cattctctct attccaataa agaaatcttc ctgcgtgagc
    73921 ttatctctaa cgcctccgat gcggcggaca agctgcgttt ccgtgcgcta tctaacccgg
    73981 acctgtacga aggtgatggc gaactgcgcg ttcgtgtctc tttcgataaa gacaagcgca
    74041 cgctgaccat ctccgataac ggcgtgggga tgacccgcga cgaagtgatt gaccatctgg
    74101 ggactatcgc taaatccggt accaaatcat tcctcgaatc cctgggttct gaccaggcga
    74161 aagacagcca gctgatcggt cagtttggtg ttggtttcta ctctgcgttt atcgtggccg
    74221 acaaagtgac cgtgcgtact cgcgcggcag gcgaaaaacc agaaaacggc gtcttctggg
    74281 aatcggctgg cgaaggtgaa tataccgttg ccgacatcac caaagaagat cgtggtactg
    74341 aaatcaccct gcatctgcgt gaaggcgaag acgagttcct cgatgactgg cgcgtgcgtt
    74401 ccatcatcag caaatactcc gaccatatcg cgctgccggt agagatcgaa aaacgcgaag
    74461 agaaagacgg cgaaaccgtt atctcctggg agaaaatcaa caaagcgcag gcgctgtgga
    74521 ctcgtaacaa gtcggaaatc accgatgaag agtacaaaga gttctacaaa cacatcgccc
    74581 acgactttaa tgatccgctg acctggagcc acaaccgcgt tgaaggtaag caggagtaca
    74641 ccagcctgct gtacatcccg tcccaggctc cgtgggatat gtggaaccgt gatcataaac
    74701 acggcctgaa actgtacgtc cagcgtgtgt tcatcatgga cgacgcagaa cagttcatgc
    74761 cgaactatct gcgcttcgtg cgtggtctga ttgactccag cgatctgccg ctgaacgttt
    74821 cccgtgaaat cctccaggac agcacggtaa cgcgtaacct gcgcaatgcg ctgaccaagc
    74881 gtgtgctgca aatgctggaa aaactggcga aagacgacgc ggaaaaatac cagaccttct
    74941 ggcaacagtt tggcctggta ctgaaagaag gtccggcgga agatttcgcg aaccaggaag
    75001 cgatcgccaa actgctgcgt tttgcttcta cccataccga ttcttctgcg cagaccgtat
    75061 ctctggaaga ctacgtttcc cgcatgaaag aagggcagga gaaaatctac tacatcaccg
    75121 cagacagcta tgcggcagcg aagagcagcc cgcacctgga actgctgcgt aagaaaggca
    75181 tcgaagttct gctgctttcc gaccgcatcg atgagtggat gatgaactat ctgactgagt
    75241 tcgacggtaa accgttccag tcggtgtcta aagttgacga gtcgctggaa aaactggctg
    75301 acgaagttga tgagagtgcg aaagaagcgg agaaagcact gactccgttc atcgaccgtg
    75361 tgaaagccct gctcggcgag cgcgtgaaag atgtccgtct gactcaccgt ctgaccgata
    75421 cgccagcgat cgtttcgacc gacgcggacg aaatgagcac tcagatggct aaactgttcg
    75481 ctgcggcggg ccagaaagtg ccagaagtga aatacatctt cgaactgaac ccggatcacg
    75541 tactggtgaa acgtgcggca gatactgaag atgaagcgaa gttcagcgag tgggtagaac
    75601 tgctgctgga tcaggcgctg ctggcagaac gcggcacgct ggaagatccg aacctgttta
    75661 ttcgtcgtat gaaccagctg ctggtttcct gatgtcatgc cggatgacct ttgtgtcatc
    75721 cggcattttt cttttcatca tctgcacttt ccgcaaatta tctcgccatt aaccgtttca
    75781 gcctcaggtg cctttcttga ggcaatcgcc tgttggtggt atcgtttatc gctttttcaa
    75841 aaaattcgac acattttaag gggattttcg caatgcgtat cattctgctt ggcgctccgg
    75901 gcgcggggaa agggactcag gctcagttca tcatggagaa atatggtatt ccgcaaatct
    75961 ccactggcga tatgctgcgt gctgcggtca aatctggctc cgagctgggt aaacaagcaa
    76021 aagacattat ggatgctggc aaactggtca ccgacgaact ggtgatcgcg ctggttaaag
    76081 agcgcattgc tcaggaagac tgccgtaatg gtttcctgtt ggacggcttc ccgcgtacca
    76141 ttccgcaggc agacgcgatg aaagaagcgg gcatcaatgt tgattacgtt ctggaattcg
    76201 acgtaccgga cgaactgatt gttgatcgta tcgtaggccg ccgcgttcat gcgccgtctg
    76261 gtcgtgttta tcacgttaaa ttcaatccgc cgaaagtaga aggcaaagac gacgttaccg
    76321 gtgaagaact gactacccgt aaagacgatc aggaagaaac cgtgcgtaaa cgtctggttg
    76381 aataccatca gatgactgca ccgctgatcg gctactactc caaagaagcg gaagcgggta
    76441 acaccaaata cgcgaaagtt gacggcacca agccggttgc tgaagttcgc gctgatctgg
    76501 aaaaaatcct cggctaattc gaaagcgcgc acggacagtc ccctcgcccc ctcggggaga
    76561 gggttagggt gaggggaaca ggcccgcaca agcaaactta tcagcaatct caggccggat
    76621 attcattcgg ccttttacaa aaacctcaat ctgcgctatt cctttttctg atttgacctc
    76681 tcacagcaat tagttcttct tcctcacttt tccgctacaa ttatcaacaa gttgaatcga
    76741 taagaggcgg taatgcgtca gactaaaacc ggtatcctgc tggcaaacct gggtacgccc
    76801 gatgccccca cacctgaagc ggtaaaacgc tatctgaaac aatttttaag cgacagacgc
    76861 gtggttgata cctcacggtt gttatggtgg ccgttgctgc gcggcgtgat tttgccgctg
    76921 cgctcgccgc gtgtggcgaa gctgtacgcc tcggtctgga tggaagacgg ctcgccgctg
    76981 atggtttaca gccgccagca acagcaggcg ctggcacaac gtttaccgga gacgcccgta
    77041 gcgctgggca tgagctacgg ctcgccatca ctggaaagcg ccgtcgatga actcctggca
    77101 gagcatgtag atcatattgt ggtgctgccg ctttatccgc aattctcctg ttctacggtc
    77161 ggtgcggtat gggatgaact ggcacgcatt ctggcgcgca aacgtagcat tccggggata
    77221 tcgtttattc gtgattacgc cgataaccac gattacatta atgcactggc gaacagcgta
    77281 cgcgcttctt ttgccaaaca tggcgaaccg gatctgctgc tgctctctta tcatggcatt
    77341 ccccagcgtt atgcagatga aggcgatgat tacccgcaac gttgccgcac aacgactcgc
    77401 gaactggctt ccgcactggg gatggcaccg gaaaaagtga tgatgacctt tcagtcgcgc
    77461 tttggccggg aaccctggct gatgccttat accgacgaaa cgctgaaaat gctcggagaa
    77521 aaaggcgtag gtcatataca ggtgatgtgc ccgggctttg ctgcggattg tctggagacg
    77581 ctggaagaga ttgccgagca aaaccgtgag gtcttcctcg gtgccggcgg gaaaaaatat
    77641 gaatatattc cagcgcttaa tgccacgccg gaacatatcg aaatgatggc taatcttgtt
    77701 gccgcgtatc gctaaagctg agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg
    77761 gcggttttca tcattcgtga ataatgcaaa aaggcatgca gcgtacctgg gtagagtttg
    77821 aactcacagg gctgctgatg cgccgctaac gtctggtaaa gcagacggct gtcatccagc
    77881 agcggatcga actccgcccc ggcaataaaa cagggcggaa cttcgcgagt gagatcatta
    77941 ttaaacagac agtaatacgg tgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca
    78001 tacatctgca aatcctgttg cgttaagcca tcccagacac cgcccaacag acgacgagtc
    78061 acggaatccc gtaatccgta aagcccatac cacagcaaaa cacccgcaat tttaccgcaa
    78121 tcgatctgtt tatcacgcaa ccacaacgca ctggcgagcg ccagcatggc acctgcggaa
    78181 tcaccggcaa agccaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa
    78241 taacaacaag cagccacaat ttcctctatc gcttgcggaa aacgcgcttc aggtgaaagg
    78301 gggtaatcaa taccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga
    78361 tcgtgggtat cgagattgcc gagaataaaa ccgcctccat gcaaataaaa tagcgtcgcc
    78421 gggctatctg gctgcggaca aaagagacgt gtttccacct gcccatattt tgttggaacc
    78481 atgtaagctc tggttgccat ttctggagcg cccgcattcc agaatcggcg ctcaagcgtg
    78541 taatactgtc gttgctcagc aatcgttccc gttgcgggcc aggacggtaa atccgactga
    78601 agagtattca caacggtctt catttcagca gaaataaggt ccagaacagg tagtttgttt
    78661 tccggcttca tacaaaaact cctttcaaat tacgtcattg taaggaacca ctgccatgaa
    78721 aatgcgatcc cgcctgctga tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg
    78781 tgttaacatt cgccgctcag ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa
    78841 cgtaaatcca aacattactt ccctgtaaat gcacgcgatc cgctgcttca gcaattccag
    78901 ccagaaaacg aaaccagcgc cgcctgggtc gtaggtattg accagacgct ggtcgatatt
    78961 gaagcgaaag tggatgatga attcattgag cgttatggat taagcgccgg gcattcactg
    79021 gtgattgagg atgacgtagc cgaagcgctt tatcaggagc taaaacagaa aaacctgatt
    79081 acccatcagt ttgcgggtgg caccattggt aacaccatgc acaactactc ggtgctcgcg
    79141 gacgaccgtt cggtgctgct gggcgtcatg tgcagcaata ttgaaattgg cagttatgcc
    79201 tatcgttacc tgtgtaacac ttccagccgt accgatctta actatctaca aggcgtggat
    79261 ggcccgattg gtcgttgctt tacgctgatt ggcgagtccg gggaacgtac ctttgctatc
    79321 agtccaggcc acatgaacca gctgcgggct gaaagcattc cggaagatgt gattgccgga
    79381 gcctcggcac tggttctcac ctcatatctg gtgcgttgca agccgggtga acccatgccg
    79441 gaagcaacca tgaaagccat tgagtacgcg aagaaatata acgtaccggt ggtgctgacg
    79501 ctgggcacca agtttgtcat tgccgagaat ccgcagtggt ggcagcaatt cctcaaagac
    79561 cacgtttcta tccttgcgat gaacgaagat gaagccgaag cgttgaccgg agaaagcgat
    79621 ccgttgttgg catctgacaa ggcgctggac tgggtagatc tggtgctgtg caccgccggg
    79681 ccaatcggct tgtatatggc gggctttacc gaagacgaag cgaaacgtaa aacccagcat
    79741 ccgctgctgc cgggcgctat agcggaattc aaccagtatg agtttagccg cgccatgcgc
    79801 cacaaggatt gccagaatcc gctgcgtgta tattcgcaca ttgcgccgta catgggcggg
    79861 ccggaaaaaa tcatgaacac taatggagcg ggggatggcg cattggcagc gttgctgcat
    79921 gacattaccg ccaacagcta ccatcgtagc aacgtaccaa actccagcaa acataaattc
    79981 acctggttaa cttattcatc gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat
    80041 caggtactga accagcattc acctcgttta acgcgcggct tgccggagcg tgaagacagc
    80101 ctggaagagt cttactggga tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt
    80161 cacgccgcat ctggcatttg gctctcgatg cctgatgcga cgctggcgcg tcttatcatg
    80221 cctacatatt tttcatattt tacatccggc aaccaccgtt taccccgtca ccacctcacc
    80281 cgccggtggc gtttccagca gttccagcat ggtacgggcg atttcacgct cgcccatcac
    80341 tacctgattc gcaccacgtt cggtgatata cgccacttca tcgtcataat gggcgcgggc
    80401 aataatctca atatccggat ttttcgcgcg ggcagatgcc acaatctcac ccgcttcata
    80461 accgttggga atcgtcagga tcagccattt tgcacattcc agatgcgcca gttgcataat
    80521 ttcttcgttc gccgcattgc ccaatactgc gcggaccccg cgctctcgca gctcatcaac
    80581 acgggttcgt gacgtctcaa tcaccaccag cggaatatca gaggcgagca atttctcccc
    80641 cagcaggctg cctacacgac cgtaacccac cagtagcgca tggttgcaaa tatccactgg
    80701 gatctgcttc tcttcttcga ttgcctcttc cagcgtctgc tcttccagcg tttcggtctt
    80761 cgccagatat ttctccagta gtgcgaacag taccgggttg agcataatcg acaggatcgc
    80821 ccctgccagt accaggtttt gtccggcctg cggcagtaaa ttcaatgcca ttcccagtcc
    80881 cgccaggata aacgcgaact caccaatctg cgccaggctg gcggcgatgg ttaatgccgt
    80941 acgttgggag tgaccaaaca gtcgcaccag gaaaaatgcg gctaacgact taccaaacag
    81001 aataatcgcc agcgtcgcca gcactgccag cggttgctga atcagaatta acggatcaaa
    81061 caacatcccg acggagacaa aaaacagcac cgcaaacgcg tcgcgcaatg gcagcgtatc
    81121 gtgggcggca cggtgactca gttcagactc gttcagtacc atcccggcaa agaacgcacc
    81181 gagtgcaaag gagacatcaa acagctctac cgcaccaaag gcaatcccta acgccagcgc
    81241 cagcaccgac agggtaaaca gctcgcgaga accggttgcc gcgctgcgtg ccataatcca
    81301 cggcaccaga cggcgaccta ccagcatcat aatggcgata aatgcgatca ctttgccgat
    81361 ggtgatcccc atatcgactg caagagtggc aaagcccaca tcgccctgtt ccatcattcc
    81421 tgccactgcg ggcagcaaca ccagcgtcag aaccattacc aggtcttcca caatcaacca
    81481 accgatggcg atttgcccac gctgactgtc aattaattgc cgttcttcaa gtgcgcgcag
    81541 taacaccacg gtactggcgg tggaaagaca taaaccgaac acgataccgg tcattaacga
    81601 ccagcccagc acggcggaga gcgccatacc cagcagcgtc gccacggcta tctgggcgat
    81661 cgcgccggga atggcgatgg actttaccgc catcaaatcc ttgagcgaaa aatgcaaacc
    81721 gacgccgaac atcagcaaaa taacgcccag ttcagccagt tccggcgcaa gcttggtatc
    81781 ggcaacaaag cccggagtga atggtcctgc cagcacaccc gctaacagat atcccaccag
    81841 aggagaaata cgtagtttat tggccagcat gccgaggata aaggcgagca caaggccgcc
    81901 aacaatggtg gtgataagcg gggtggcgtg atgcattccg tctccttttc ctggtggtta
    81961 ttgtccattt ttggccggga aaaccaaaat tacaggtaat agtttatgac aatttcattg
    82021 atgatgttca tgaataatta ttgaattttg cagaaaaatg gaattagctg caaaaaaagc
    82081 acggatcgga aaacagaagg cggtttaaca gaggaaaggc ttatggcgac tgtaaaacga
    82141 tgcagccaaa gtttgccttt ggctgcaatg aaatcagtct ttatgccggt tatcaggcag
    82201 gaatatggtc aacatcccca atagtggcag gaaagcacag attttataga ctaactcgat
    82261 actggtgtga tcggcgataa gccccagaac tgccgctccc agacctccca taccaaaagc
    82321 aaaaccgaaa aagagtccag aaaccatacc gatacgtccc ggaagcagct cctgagcgta
    82381 aaccagaatg gcagagaatg ccgaagcgag gataaatcca ataatcaccg ttaaaacccc
    82441 cgtccagtgc aggctggcgt agggtaaaat cagcgtaaac ggcgcaacgc cgaggataga
    82501 gccccaaatc acatatttcc gcccaatttt atcccctaca ggcccgccga tcaccgtacc
    82561 tgccgcaacg gcaaacagga aggcaaacag atgaagctga gcattctgga tagataatcc
    82621 gaatttttgc atcagataaa aggtgtaata gctgctgatg ctcgccatat agaaatattt
    82681 cgagaaaatg aggattaaca gaatgctgac cgccagtacc actttgttgc gcggcagtgg
    82741 attgataatc gtcgctttgg gttttccttt attcattcgg tgctgtgccg agtaccaacg
    82801 gctgatttgc gccaacacca cgatcgccag cagtgccgca agcacaaacc aggcaacgtt
    82861 gcctttgcca taaggcgcga taatcaccgc cgccagcaag ggtcccaggg aactgccaaa
    82921 gttgccgccg acctgaaaga tagattgcgc caggccatgc cgcccgccgg aagccatacg
    82981 ggctacgcga gaagattccg gatgaaagac cgatgaaccg gtaccgacca gcgccgccgc
    83041 cagcagaact gcgccaaaac tgcccgccag cgcaagcagc accagaccac ttaaggtaaa
    83101 gcacatgcca attggcaacg accacggcat cggatatcta tcggtccagt agccgaccac
    83161 tggttgcagt agcgaagagg cgagctggaa ggtgagggtt atcatgccaa tctgcataaa
    83221 tgtcagagaa aattctgact gaagcagcgg ataaatcgcc agaatcagcg attggatcat
    83281 gtcgttcagc agatgtgaga ggctgatagc acctaaaata ccaaacgatg ttcgggcctt
    83341 ggtcgttgac gcagccgcgc ccgccacagg ctggggttgt tcactcattg ccataggaaa
    83401 gtcacttttt cagggttgcg atgtaaagaa tgatcttatt tgtgattatt acccgactaa
    83461 catacctgta tgcgtcgcct gaaggaagtc tcaacgccga atacagaatt tctaatctgg
    83521 atgcagattt atcttcaccg gacgcagact tgtctatgat gtcgcgtcat actatttttc
    83581 aacacgttga aatcaggtca gggagagaag tatgaaatta ttgcagcggg gcgtggcgtt
    83641 agcgctgtta accacattta cactggcgag tgaaactgct ctggcgtatg agcaggataa
    83701 aacctacaaa attacagttc tgcataccaa tgatcatcat gggcattttt ggcgcaatga
    83761 atatggcgaa tatggtctgg cggcgcaaaa aacgctggtg gatggtatcc gcaaagaggt
    83821 tgcggctgaa ggcggtagcg tgctgctact ttccggtggc gacattaaca ctggcgtgcc
    83881 cgagtctgac ttacaggatg ccgaacctga ttttcgcggt atgaatctgg tgggctatga
    83941 cgcgatggcg atcggtaatc atgaatttga taatccgctc accgtattac gccagcagga
    84001 aaagtgggcc aagttcccgt tgctttccgc gaatatctac cagaaaagta ctggcgagcg
    84061 cctgtttaaa ccgtgggcgc tgtttaagcg tcaggatctg aaaattgccg tcattgggct
    84121 gacaaccgat gacacagcaa aaattggtaa cccggaatac ttcactgatg tcgaatttcg
    84181 taagcccgcc gatgaagcga agctggtgat tcaggagctg caacagacag aaaagccaga
    84241 cattattatc gcggcgaccc atatggggca ttacgataat ggtgagcacg gctctaacgc
    84301 accgggcgat gtggagatgg cacgcgcgct gcctgccgga tcgctggcga tgatcgtcgg
    84361 tggtcactcg caagatccgg tctgcatggc ggcagaaaac aaaaaacagg tcgattacgt
    84421 gccgggtacg ccatgcaagc cagatcaaca aaacggcatc tggattgtgc aggcgcatga
    84481 gtggggcaaa tacgtgggac gggctgattt tgagtttcgt aatggcgaaa tgaaaatggt
    84541 taactaccag ctgattccgg tgaacctgaa gaagaaagtg acctgggaag acgggaaaag
    84601 cgagcgcgtg ctttacactc ctgaaatcgc tgaaaaccag caaatgatct cgctgttatc
    84661 accgttccag aacaaaggca aagcgcagct ggaagtgaaa ataggcgaaa ccaatggtcg
    84721 tctggaaggc gatcgtgaca aagtgcgttt tgtacagacc aatatggggc ggttgattct
    84781 ggcagcccaa atggatcgca ctggtgccga ctttgcggtg atgagcggag gcggaattcg
    84841 tgattctatc gaagcaggcg atatcagcta taaaaacgtg ctgaaagtgc agccattcgg
    84901 caatgtggtg gtgtatgccg acatgaccgg taaagaggtg attgattacc tgaccgccgt
    84961 cgcgcagatg aagccagatt caggtgccta cccgcaattt gccaacgtta gctttgtggc
    85021 gaaagacggc aaactgaacg accttaaaat caaaggcgaa ccggtcgatc cggcgaaaac
    85081 ttaccgtatg gcgacattaa acttcaatgc caccggcggt gatggatatc cgcgccttga
    85141 taacaaaccg ggctatgtga ataccggctt tattgatgcc gaagtgctga aagcgtatat
    85201 ccagaaaagc tcgccgctgg atgtgagtgt ttatgaaccg aaaggtgagg tgagctggca
    85261 gtaatccgaa agtgccggat gtttgcatcc ggcacaatgc ttaatcgcgg cgggcgatat
    85321 cagcaaattt ggcatcgagg atctttgcca gatcgcctgc cgccagttcg atatccagtc
    85381 cgcgcttgcc gccggaaaca taaatagtgg caaattcttg tgcgggggcg tcgataatcg
    85441 ttggcagacg ttttttctgc cccagtgggc taatcccccc aaccaggtat cccgtcgaac
    85501 gctgcgcgac catcggatcg gccatctcaa ctttcttggc acccagcgct tttgctactt
    85561 ttttaagatc cagttgacct gcgaccggcg taacggccac ggcaaggtgt ttcatatcac
    85621 cgttcactgc caccagcagc gttttgtaga cctgatccgg attcaaacct aattttttga
    85681 cgacttcatc gccaaaattg gtttcagccg gatcgtgctc gtaggtatgg atttgaaacg
    85741 aaatcttgtt tttttcgagt aatttaactg cgggtgtcat ggtaattttc ctgccttaaa
    85801 caaaaaaacg aagcaagcat acgcctgatg gcggtccaaa aaaaagagtc atcttgccta
    85861 agagtattgg caggatggtg agattgagcg acaatcgagt tacaccgtcg cttaaagtga
    85921 cggcataata ataaaaaaat gaaattcctc tttgacgggc caatagcgat attggccatt
    85981 tttttagcgc aacatttgcg gcaaattccc ttctccatac aggtgtagtg caccgaccgc
    86041 gaccacatat cgccccggcg gcatggcgcg taatttatcc cgccaggcga gatttcgctg
    86101 atgcatcagc acatcgtaca gcgactgact gaacgtattg ggcagcgtta tttcattatt
    86161 ttgcggcggc gcattcagcc accagctcat catttgttgc agcaaccgtg cgttggtatg
    86221 ccagtgggtc agcgtatcgt ccagcagcgc cagtcctttg tcagggagct ggagcaacat
    86281 ggcaatctgg ttttcagccc cttccagttc aatcacgggt ttatgttgtt gcttcgccgc
    86341 ctgcaatagc tggtaatcga taccgtattc tgcccgcagc cccagttttt gtgcctgcgt
    86401 cgcctgaaga accatcgcga tttgccacag cggttgggta gaaaagagtg agggagaaat
    86461 gcccatctcc tggctaatgt gctgcagatt ttggagttgc tcctcgctaa tgcgctcttc
    86521 cagcgcctcg caggcaggca aattagcaaa aggcgtatcg ctggtggaaa catccgcctc
    86581 gacgatcagc gcatcggcgt ttttgagctt tttgagcaaa cgggtgggca ggggagccat
    86641 atcgtggcta cccatatgaa tactgccaat cagatgaaaa tggcgattgc cggggagggt
    86701 gatatcgatg gcaggccagg tgtaatgatt accgcgcagc gcggcccaaa gcgtttttac
    86761 ccggtacaac agatccatac gacctccctt tgtgaaatat catgctagcg cgcggtgagg
    86821 gatggcgcaa atggagttgg ggacttgttt aatgtgtttg tatgattcag tatgttcttg
    86881 catcgctatt cacagggaag caacagttaa aaaccatgaa acaggcaaca agaaaaccga
    86941 cgacacccgg agatattctt ctctatgaat atctggaacc gctcgatttg aaaatcaatg
    87001 agttagcaga gttgctgcat gttcatcgta atagcgtcag tgcactgatc aataacaatc
    87061 gtaaactcac cactgagatg gcattccgtc tggcgaaagt ttttgatacc acagtcgatt
    87121 ttaggctaaa cctccaggcg gcagttgatc tttgggaagt tgaaaacaac atgcgcaccc
    87181 aggaagaatt gggacggatt gaaacagtgg ctgaatattt ggcacgccgt gaagagcgtg
    87241 caaaaaaggt cgcgtaaggc caaaacatcg cttcatctcg taggccggat aaggcgttta
    87301 cgccgcatcc ggcagtcatg cgtcgatgcc aaatgcgccg ccctaaagca gcgcatccgc
    87361 aatgacgtac ttattccttc ggtttaaacc gcagcaaccg gttggcgtta ctcactacgg
    87421 taatcgacga gagcgccatt gccgctccgg caactaccgg gttaagcagt gttccagtaa
    87481 acggccacaa aataccggcg gcgaccggaa taccgatgct gttgtagata aaggcaccga
    87541 gcaggttctg cttcatgttg cgcagcgttg cgcgggaaat cgcgagcgca tccgcaacgc
    87601 ccatcaggct atggcgcatc agggtaatcg ccgcggtttc aatggcaaca tcactgccgc
    87661 cacccatcgc aatgccgaca tccgcctggg ccagcgctgg cgcgtcgtta atgccgtcgc
    87721 ccaccattgc cacctgacgt ccttcacttt gcagacgttt gatcgcttcg gctttaccgt
    87781 ccggcagcac tccggcaatc acttcatcaa ttccagcttc tttggcgatc gcattggcgg
    87841 tggttgggtt atccccggtc aacatcacca gacgatatcc cgctttatgc aggcgttgca
    87901 gcgccgccac gctatcacta cgcaacggat cgcgtacagc cagcagggct actgctttcc
    87961 cgtcaatcgc cagcagcaca ggcgttgctc cttgcgatgc ctgagcagta atctccgctt
    88021 cgatagcttt ggtaccaacc tgttgctcat ttaacagcgc ctgattgccc agcaataacg
    88081 catgaccttc agcttcaccg ctcacgccca gcccgcgcaa tgtgcggaaa ccgttgacct
    88141 gcggcagctg catatcacct gctttatcga ggatcgcccg tgccagcgga tggctggaac
    88201 cttgctccag tgccgccgcc agacgcaatg cctgcgcttc atcaacatca gcaaacgttt
    88261 tcactgcgac aacctgcggc ttcccttcgg tcagcgtccc ggttttatcg aacacgacgg
    88321 tatcgagtgt actggcgcgt tgcagcgcgt cggcgtcccg caccagcacg ccaaactcag
    88381 ccgcccgccc gacgccggaa ataatcgaca tcggcgtcgc cagccccagc gcacacggac
    88441 aggcgataat cagtaccgtg gtggcaatca ccagggtata aacaatttgc ggtgccggac
    88501 caaagaagta ccagattgcc gcactgacca gcgcaatcac caccaccaca ggtacaaata
    88561 cggctgagat tttatccgcc agctgaccga tttctggctt gctgctctgg gcctggcgca
    88621 ccatgcgaat gatccgtgac agcgtggtat ggctgccaac cgcactggca cgaaacagca
    88681 cgctaccgtc ctgtaccact gtcccggcat ggacgctttc gccttcgcct ttttgctgcg
    88741 ggattggttc gcccgtcagc atcgcttcat ccagccatgc ttcgccctgg gtaatctcgc
    88801 catctaccgg cacgcgatcg ccggtcgtca ggcgcagcaa catacctggc tgcacttccg
    88861 ccagaggcac gcttttttca ccttcgtcag taaccaggcg cgccgtcggt ggggttaaat
    88921 cgagtaattt ctccagcgcc ttagaagaac gctgacgtgc gcgtgcttcc agcatatggc
    88981 cgagattgat cagaccgata atcatcgcgc tggcttcgta ataaagatgt cgcgcttcca
    89041 tcgggaacca ctgcggccac aggttgacgc tcatcgaata gagccacgcc acgccagtac
    89101 ccagcgccac cagcgtatcc atcgtcgctg caccgttcag caggcttttc catgcactgc
    89161 ggtaaaaatg gccgccggcg aaaaccatca ccgccagggt tatcaggccg ataaccaacc
    89221 acaggctgcg gttgtcagcg gtgaccatca tgttatcgcc aatcatcccc cagaccatca
    89281 ccgggatacc caccgccagt gcgacaattg cctgccagcg gaagcgcttc atcgtagcaa
    89341 cggcggtttc ctgctggcgc tcacggcgtt tcgcgtcatc ttcaatcgct tccgcgccgt
    89401 agcccgcttt ttccaccgcc tgcactaaat cttgtgggga ggcactgccc atcaccagcg
    89461 cagtacgctc cgccaggttt acccgtgcct gagtgacgcc cggtacgctt tgcagcgcat
    89521 tttgtacgcg ggtgacacag ctggcgcagc tcatgccgct cagcagcaac tgctggctgt
    89581 catcgtcatc ggcggtcgct gccggaagcg cctcagaaac cgctgtcagt gcttccgacg
    89641 ggattgatga ctccgccagc ggtttagcct ttgggtggct tacagatgcg tcataacccg
    89701 cttgtttgat ggtttcgatc agctgttctg cactggcagt cccggtaacg tgcgcttcag
    89761 tgatagacac atccgcttgt tccacatccg ggcgctgttc gagactttct ttcacgcgtt
    89821 taacgcagtg accgcaggac aggccgtcca gggtcaggtc gatagtttgt gacataaaac
    89881 actcctttaa gacagttttg actggctgtg ataaaggtta aaccttccag caaggggaag
    89941 gtcaagaaat taataaacca ggcgagtaaa agtccgtaaa gattaaaaaa ttggctcaat
    90001 ttgcatcagg attagacatt tatctctttg ttttcctgaa gttaagttgc gggtgctaag
    90061 ttaaatcaag atgcctgaaa atcggcaccg gggtgaggaa ttacctcccg catctataaa
    90121 aaggagttaa caaaagatgt tagatgcaaa caaattacag caggcagtgg atcaggctta
    90181 cacccaattt cactcactta acggcggaca aaatgccgac tacattccct ttctggcgaa
    90241 tgtacctagt caactggcag cagtggctat tgtgacctgc gatggcaaca tctatcgcgc
    90301 gggtgatagt gattaccgct ttgcgctgga atccatctcg aaagtctgta cgttagccct
    90361 tgcgttagaa gatgtcggcc cgcaggcggt acaggacaaa attggcgctg acccgaccgg
    90421 attgcccttt aactcagtta tcgccttaga gttgcatggc ggcaaaccac tgtcgccact
    90481 ggtaaatgct ggcgctattg ccaccaccag cctgattaac gctgaaaata ttgaacaacg
    90541 ctggcagcga attttacata tccaacagca actggctggt gagcaggtag cgctctctga
    90601 tgaagtcaac cagtcggaac aaacaaccaa cttccataac cgggccattg cctggctgct
    90661 gtactccgcc ggatatctct attgtgatgc aatggaagcc tgtgacgtgt acacccgtca
    90721 gtgctccacg ctcatcaata ctgttgaact ggcaacgctt ggcgcgacgc tggcggcggg
    90781 tggtgtgaat ccgctgacgc ataaacgcgt tcttcaggcc gacaacgtgc cgtacattct
    90841 ggccgaaatg atgatggaag ggctgtatgg tcgctccggt gactgggcgt atcgcgttgg
    90901 tttaccgggc aaaagcggtg taggtggcgg tattctggcg gtcgtccctg gcgtgatggg
    90961 aattgccgcg ttctcaccac cgctggacga agaaggcaac agtgttcgcg gtcaaaaaat
    91021 ggtggcatcg gtcgctaagc aactcggcta taacgtgttt aagggctgat catgatgaac
    91081 acggaaggta ataacggtaa caaacctctc ggtctatgga acgtcgtttc catcggcatt
    91141 ggggcaatgg tgggggcggg gatcttcgcg ctgctggggc aggctgcatt gctaatggaa
    91201 gcctcgacct gggtcgcctt tgcttttggc ggtattgtgg cgatgttttc cggttatgcc
    91261 tatgcgcgtc tgggggcgag ctatcccagc aatggcggca ttatcgactt ctttcgtcgc
    91321 ggattaggca acggcgtctt ttcgctggcg ctctcgttac tgtacctgtt gacgctggcg
    91381 gtgagcatcg ccatggtcgc ccgtgctttt ggcgcttatg ccgtgcagtt tttgcatgaa
    91441 ggcagccagg aggagcacct tattttgctc tacgcgttgg ggatcattgc ggtgatgacg
    91501 cttttcaact ccttaagcaa ccatgcggta gggcggctgg aagtgatcct cgtcggcatt
    91561 aaaatgatga tcctgttatt gctgattatt gccggtgtct ggtcgctgca accggcgcat
    91621 atttccgtct ctgcgccccc cagctccggt gcgttcttct cctgtatcgg gataaccttc
    91681 cttgcctatg cgggctttgg catgatggcg aacgcggcgg ataaagtgaa agatccgcag
    91741 gtcattatgc cgcgggcgtt tctggtggcg attggcgtta ccacgttgct ttatatctcg
    91801 ctggcactgg ttttgcttag cgatgtatcg gcattagagt tagaaaaata tgccgatacc
    91861 gccgtagcgc aggctgcttc tccgctgctc ggacatgtgg gttatgtgat cgtcgtcatc
    91921 ggcgctttac tggcgacggc ttcagccatt aacgcgaacc tgttcgccgt gtttaacatc
    91981 atggacaaca tgggcagcga acgcgaactg ccgaagctaa tgaataaatc cctgtggcgg
    92041 cagagtacct ggggcaacat cattgtcgtg gtgttgatta tgctgatgac ggcggcgcta
    92101 aatttaggct cactcgccag tgttgccagc gccacctttt tgatttgcta cctggcggtg
    92161 tttgtggtgg cgatccgcct gcgtcatgat attcacgcct cgttgccgat tcttatcgtt
    92221 ggtacgttgg tgatgttgtt ggtgatcgtt ggctttatct acagcctgtg gtcccagggt
    92281 agccgtgcgt tgatatggat tattggctca ctcttactca gccttattgt ggcaatggtc
    92341 atgaagcgca ataaaaccgt ataacatctc tctgtgcgca gtacttcctg tattattgtg
    92401 gtggcggtcg atattcgcac tggcaaaaaa acgtgcttga atatctgttg aaacccttta
    92461 acaaagcaca ggaggcgttg cgcgaacgat gaacatcagc gatgtagcaa aaattaccgg
    92521 cctgaccagc aaagccattc gcttctatga agagaagggg ctggtgacgc cgccgatgcg
    92581 cagcgaaaac ggttatcgca cctacacgca gcagcatctc aacgaactga ccttactgcg
    92641 ccaggcacgg caggtgggct ttaacctgga agagagcggc gagctggtga atctgtttaa
    92701 cgacccgcag cggcacagcg ccgacgtcaa acggcgcacg ctggagaagg tggcggagat
    92761 cgaacgacac attgaggagc tgcaatccat gcgcgaccag ctgctggcac tggcgaatgc
    92821 ctgccctggc gatgacagcg ccgactgccc gattatcgaa aatctctccg gctgctgtca
    92881 tcatcgggca gggtgattaa gacgagacgg ctcggatatg cagggttatc ccttctatcg
    92941 caatgacttc aacatgcgta cctgcaccga gatcctcgct ggcgctgaca ggccatgaac
    93001 tgtcaccgac gcgcatatga ccgcgcccgt tgaccagcgg agattccagc acaaaacgtc
    93061 ggccaatcag ctgctgcccg cgctggttta aatgactatc gctgtgcttt tgttcgcgca
    93121 cccgccgcga caaccatttc caccacagcc aggcggcgag cagcgtcagg atggcaaaca
    93181 tcaccccttg ccactcccaa cccagcggca ccagccagac caccaggccg gtaatcaccg
    93241 ctgacacgcc gctccacagc aaataaccat ttccgcccag catctcggct gccagcagca
    93301 acccgccgag actgagccag aaaatatgtg gatgaacgac cattaactcc atcatggctg
    93361 agtccgtttg ttggcgctgt ctttcaccag ctcggcaatc ccggcaatcg accccatcag
    93421 gctgctggcc tctaatggca tcatcactac tttgctgtta ctggaggaac cgatctgctg
    93481 taacgcttcg gtgtatttct gcgctacgaa gtagttcacc gcctgaatat caccggaggc
    93541 aatggcttca gataccattt tggtggcgcg ggcttctgct tcggcagaac gttcacgcgc
    93601 ttcggcctgt aagaatgccg actgacgttc accttccgct ttcagaattt gcgactgttt
    93661 ttcaccttcc gctttgagta tttccgcctg acggatccct tccgcttcaa ggatgtaagc
    93721 gcgtttggta cgttccgctt tcatctgcgc gttcattgaa gagataagct ctgccggtgg
    93781 gcgcacgtcg cgaatttcaa tacgggtgac tttaatcccc cacgggttgg tggcttcatc
    93841 gacaatacgc agcaggcgtg agttgatgct gtcgcgctga gagagcattt cgtcaagttc
    93901 cattgaaccc aacacggtac ggatgttagt catggtcagg ttgatgatcg ccagctccag
    93961 attgctgact tcataagccg cgcgcggcgc gtcaatcacc tgaataaaac agactgcgtc
    94021 gatggtaacg ttggcgttat ctttcgagat aacttcctgg gaagggatat cgagcacttg
    94081 ctccatcata ttgatcttgc gaccaatgcg atccataaac ggcaccacca gactgagccc
    94141 cggctgtaac gttttggtat agcgaccaaa acgttctacc gtccattgat agccctgcgg
    94201 cacgattttg acgcccgcgc cgacaatgac cagcgcgaca aaaatgagaa tcgggataaa
    94261 gataagcatc ggaaaaacct cctgttgtac cgtccataat cagcaaaatt gctgcttgat
    94321 taaacaaatt atacctgatt actgaaagag agttccccct tattcctgcg aaggataaac
    94381 tgtttttagt aaaaatcaga aaaagggaac agtgatgcag gaaaatagtc ctttgcttca
    94441 gctacaaaac gtaggatatc tggcgggtga tgcgaagatt cttaataaca tcaatttttc
    94501 gctgcgtgct ggcgaattta agttaattac cggtccttcc ggttgtggca aaagtacgct
    94561 gctaaaaata gttgcttcat tgatcagccc aaccagcgga acgttactgt ttgaaggtga
    94621 ggatgtcagc acactaaagc cagaaatcta ccgccagcaa gtctcttact gcgcccagac
    94681 gccgacgctg tttggcgata cggtatacga taatctgatc tttccctggc agatccgtaa
    94741 ccagcagcct gacccggcca tttttctcga ttttctcgaa cgcttcgctt tgccggacag
    94801 cattttgacg aagaatatcg ccgagctatc tggtggtgaa aaacaacgca tctcattgat
    94861 tcgtaacctg caatttatgc cgaaggtttt attgctggat gaaataacca gtgcgctgga
    94921 tgaaagtaat aaacataacg tcaatgagat gatccatcgt tatgtgcgcg agcaaaatat
    94981 tgccgtgctg tgggtgacac acgataaaga cgaaattaat catgcggata aagtgattac
    95041 actgcaaccg catgccggag aaatgcagga agcacgctat gaactcgcat aatattacta
    95101 acgaatcatt agcactggca ttaatgctgg tggtggtggc aatcttaatt agccataaag
    95161 aaaaactggc gctggagaaa gatattctct ggagcgtcgg gcgagcgata attcagctga
    95221 ttattgtcgg ctatgtgctg aagtatattt tcagcgtgga tgatgccagc ctgacattat
    95281 tgatggtgtt atttatctgc tttaatgcgg cgtggaacgc gcaaaaacgc agtaaatata
    95341 ttgctaaagc ttttatctca tcgtttattg ccattacggt cggggcggga attaccctgg
    95401 cggtgctgat tctctcaggg tcgattgaat ttatcccgat gcaggtgatc cctatcgccg
    95461 ggatgattgc cggtaacgcc atggtagcgg tggggttgtg ttacaacaat ttagggcaac
    95521 gggtcattag cgaacagcaa cagattcagg agaaactgag tcttggtgcg acgccgaagc
    95581 aggcttcagc gatattgatt cgcgacagta ttcgcgcggc tttaattccg acggtcgatt
    95641 cagcaaaaac ggttggctta gtgagtttac caggaatgat gtccgggctg atatttgccg
    95701 ggattgatcc ggtgaaggcg attaaatatc agattatggt gacctttatg ctgctctcaa
    95761 ccgccagctt gtcgaccatt attgcctgct atttaaccta tcgtaagttt tataattcgc
    95821 gccaccagtt ggtggtgacg caattgaaga agaaatgatg cgatgccgga tgcgcagcat
    95881 cgcatccggc gttgtggttc atgtgccgat caacggaata tcaatacaac aatgcataca
    95941 gctggcggcg atacttcgac gccagtgcat cacccgtacc cagcgcagcg aggatctcct
    96001 ggaacgtttt acgcgtctga ccgtctgcgg cggtgagatc tttacgcaga tgcccgaaca
    96061 gcaactccag cgcctcttca ttgcgcccaa cctgatgcag ttgcagcgcc agttgcgtcg
    96121 ccagtgcggc atcttctgga ttctccgcca cctgctgttg caactgttga atttccggag
    96181 tatcagccgc ctgcttcagc agttcgattt gcgccaccag cccctggtag cgggtgtcct
    96241 gatcctgcaa cggaatggtt ttcagcaccg cttccgcatc ttcagaacgg ttcagcgcaa
    96301 tcagcgtttc tgccagcagc aggccgatct ccccgttctg attcgacaac tgccaggcgt
    96361 ctttcagcaa tggcagggca tcggtgtaat tgctttcctg catcagttgc atcgcctgct
    96421 gcgctttcag ctcttcttcg cgcggcagca ctttatccag cagggcgcgg atcgcctctt
    96481 ccggttgcgg cccctggaag ccatctaccg gttgcccgtt ctggaacaga tacacggtcg
    96541 gaatcgcgcg cagaccaaac tgggcggcaa tcatctgctc cgcgtcgcag tccagcttcg
    96601 ccagaataaa ttgcccgttg tactgcgccg cgaggctttc caggatcggg gttaactgca
    96661 aacagtgctg gctacgttca gaccaaaaat agaacagcac cggagtggtc atcgactgtt
    96721 caagaacctg ttgcaggtta gattcgttaa tgttgacaat attttctacg gacatggagt
    96781 cgctctctgt tgtcgatatt ttctttgaca tgggggctta agcgcgcgct tcaactcacc
    96841 cctgcaatat tttgtccatc acgcgccccg gcagcaggcg cttaagcacc attactgccc
    96901 aggtcaccag cgttaccgga tagcgcatct tcggcttctc gctaataaaa gcatggcgta
    96961 ctttgtccac caccgcttcc ggtcccaacg taaagcgggc ggcgatgccg ggattttcga
    97021 ctggtttatc actttgcgtc tggttgacgt tgtcggtgaa gcgagtacga atgggaccgg
    97081 gttcgatcag gctgacttta attccgctgt ggcgtagctc catgcgcagc gcgtctgacc
    97141 acgcctccag cgcatattta ctggccgcgt aagcgccacg acccggcgtg gagattaatc
    97201 ccatcaccga tgatgtcatc acaatacgcc cttcaccgtg cggtaacatc gcgggtaaca
    97261 ggcgcatggt gagctggtgt gcgccgaaaa agttggcgga aaactgctgt tccatctgcg
    97321 cacggctgat ggtggaaagg gggccataca tgccgaatcc ggcattgtta aagatcccat
    97381 acagacaatt atcggtcagg gcgatcacct cgtctgctgc gcgatcaaca ctttctggtg
    97441 aatccagatc gatcaacacg ccggtaaatc ccatgttgtt catacgctca acatcatccg
    97501 gttttcggca acctgccagc acatgaaaac cctggcgttt taattcgagc gcgctttcca
    97561 ggccaattcc actggaacat ccggtaatta agaccgattt ttgcataact ttacctgtca
    97621 ggatctccgt tgctttatga gtcatgattt actaaaggct gcaactgctt cgccatccag
    97681 tcggcaataa acggctgggc gtcgcggttg ggatgaatac cgtcatcctg catccattgt
    97741 ggcttgaggt agacctcttc cataaaaaag ggcagcagcg gaacatcaaa ctctttggcg
    97801 agtttggggt aaatggcgct aaaggcttca ttataacggc gaccatagtt tgcaggcaga
    97861 cgtatttgca ttaacaatgg ttcagcgttg gcggctttga catcctgcaa aatctggcgc
    97921 agcgtttgct cggtttgctg tggctgaaaa ccacgcaaac cgtcattgcc gcccagttca
    97981 accagcaccc agcgcggctg atgctgtttc agcagagccg gaaggcgcgc cagtccttgt
    98041 tgcgaggtgt cgccgctgat gctggcatta actaccgacg ttttactctg ccacttatca
    98101 ttcaacaagg caggccaggc cgcgctggca gacattcgat acccggcgct caggctatca
    98161 cccagaatca ataacgtgtc cgctgcggcg gcacggaagg ttaacaggac caggaacagg
    98221 aagggcaaat gccagcggaa aacattgttg aagttcatca tcttaagaag tccgtcggtc
    98281 agggggagca tgaactctcc atcctcaccg gagttgagct ggttgtcaaa cgtggcgaga
    98341 ccatcgcact ggtgggcgag tcgggatcgg gtaagtcaac cttgctggcg atcctcgccg
    98401 ggcttgatga cggcagcagt ggcgaagtga gtctggtggg acaaccgcta cataatatgg
    98461 acgaagaagc gcgggcaaag ttgcgcgcga agcacgtcgg ctttgttttt cagtcattta
    98521 tgttaattcc tacccttaac gcgctggaaa acgtcgagct tccggctctg ctgcgcggtg
    98581 agagtagcgc ggaaagtcgt aacggggcga aagcgttgct cgaacagtta gggctgggta
    98641 aacgtctgga tcatcttccg gcacagcttt ccggcggtga acagcaacga gtggcgctgg
    98701 cacgagcctt taatggtcga cctgatgtgc tgtttgccga cgaacccacc ggcaaccttg
    98761 accgccagac gggcgataaa attgccgacc tgctgttttc cctcaaccgt gaacatggca
    98821 ccacgttgat tatggtgacc cacgacctgc aactggcggc acgctgcgac cgctgcttac
    98881 ggctggtgaa cgggcagttg caggaggaag catgattgca cgttggttct ggcgcgaatg
    98941 gcgttcgccg tcgctattaa ttgtctggct ggcgctaagc ctggcggtgg cctgcgtgct
    99001 ggcgctgggc aatatcagcg atcgcatgga gaagggctta aaccaacaaa gccgggagtt
    99061 tatggcgggc gatcgggcgt tgcgcagttc gcgcgaagtg ccgcaagcat ggctggagga
    99121 agcgcaaaag cgcggcctga aagtcggcaa gcagctgact ttcgccacca tgacatttgc
    99181 aggcgacaca ccgcagctgg cgaacgtcaa agcggtggat gatatctacc cgatgtatgg
    99241 cgatctgcaa actaatcccc ctggcctgaa accgcaggcg ggcagcgtat tgctggcccc
    99301 acgcctgatg gcgctgctta acctgaaaac gggcgacacc attgacgtgg gcgatgccac
    99361 cttgcggatt gccggagaag tgattcagga accggattcc ggttttaacc ccttccagat
    99421 ggctccgcgt ctgatgatga atctggcgga tgtcgataaa accggagccg tgcagccggg
    99481 gagtcgggtc acctggcgtt ataaattcgg cggcaacgag aaccagctcg acggctatga
    99541 gaaatggttg ttacctcagc ttaaacccga acaacgctgg tacggtctgg aacaggacga
    99601 aggcgcgctg gggcgatcga tggaacgctc gcaacagttc ctgctgcttt cggcgcttct
    99661 gaccttgctg ctggcagtgg cagcggtggc ggtagcgatg aatcattact gtcgcagtcg
    99721 ctacgatctg gtggcgatcc tcaaaacgct gggggcaggg cgagcgcaac tgcgtaagca
    99781 aatcgtcggt cagtggttga tggtgctggc gctttcagcc gttaccggtg gggcgatagg
    99841 cctgttgttc gaaaacgtgt tgatggtgct gctcaagccc gttctgcctg ctgcactacc
    99901 gccagccagc ctctggccgt ggctgtgggc gctgggtacc atgacggtca tctcgctgct
    99961 ggtggggcta cgaccgtacc gcctgttgct ggcaacgcag cctttacgcg tattacgtaa
   100021 tgatgtggta gcgaacgtct ggccgctgaa gttttatctg ccgattgtca gtgtggtggt
   100081 tgtgctgctg ctcgccggat taatgggtgg cagcatgctg ctttgggcgg tgctggcggg
   100141 cgcggtagta ctggctttgc tgtgcggtgt gctgggctgg atgctgctga atgtacttcg
   100201 ccgcatgacg ctgaaatcgc tacctctgcg cctggcggtt agccgcctgt tacgtcagcc
   100261 gtggtcaaca ttaagccagc tttcggcatt ttcgctctcc tttatgctgc tggcgctgtt
   100321 gctggtattg cgtggcgatc tgcttgaccg ctggcagcag cagctacctc cagaaagccc
   100381 gaactacttt ttaattaaca ttgccacaga acaggtagaa ccgctaaaag cgttcctcgc
   100441 ggaacatcag atagtcccgg aatcatttta tccggtagtg cgggcgcggc tgacggcgat
   100501 taacgataag ccgacagaag gtaatgaaga tgaggcgctt aatcgcgaac tcaatcttac
   100561 ctggcaaaat acgcggcccg atcataatcc gattgtcgcc ggtaactggc cgccaaaagc
   100621 cgatgaagtg tcgatggaag aggggctggc gaaacgctta aacgttgccc tcggtgatac
   100681 cgtgactttt atgggcgata cccaggagtt ccgcgctaaa gtgaccagcc tgcgcaaagt
   100741 ggactgggaa agtctgcggc ctaatttcta ttttattttc ccggaagggg cattagacgg
   100801 gcaaccgcag agctggctta ccagtttccg ctgggagaat ggcaacggca tgttgacgca
   100861 actcaaccgc cagttcccga ccattagcct gttagatatt ggcgcgattt taaaacaggt
   100921 cggtcaggtg ctggagcagg taagtcgggc gctggaggtg atggtggtgc tggtcaccgc
   100981 ctgcggtatg ttgctgttgc tggcacaggt gcaggtggga atgcgccagc gtcatcagga
   101041 gctggtggta tggcgcacac tcggtgcggg gaaaaaactg ctgcgcacga cattgtggtg
   101101 tgagttcgcc atgcttgggt ttgtttccgg cctggtggcc gcaattggtg cggaaacggc
   101161 actggcagtg ttgcaggcga aagtgtttga tttcccgtgg gagccagact ggcgattgtg
   101221 ga
//
LOCUS       DAZGAN020000003        89062 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGAN020000003 DAZGAN020000000
VERSION     DAZGAN020000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 89062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 89062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.003"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..655
                     /locus_tag="ACHKYO_RS01130"
                     /old_locus_tag="pgaptmp_000226"
     CDS             <1..655
                     /locus_tag="ACHKYO_RS01130"
                     /old_locus_tag="pgaptmp_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
                     /protein_id="HGU4427129.1"
                     /translation="SDNPLTINIFKGLFRCQCGASVHPTGTKNKYQGVYRCNNHLDGR
                     CDVPPLKRKPFDKWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLE
                     MDDITELKTQVKGLNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLTTKAGRVECQL
                     ILSKHLKGLTLGKDSVTVTLQNDTEITIPTDPLPLNDGTSIFEIAEKNLLNIDTFQL"
     gene            complement(686..1474)
                     /locus_tag="ACHKYO_RS01135"
                     /old_locus_tag="pgaptmp_000227"
     CDS             complement(686..1474)
                     /locus_tag="ACHKYO_RS01135"
                     /old_locus_tag="pgaptmp_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HGU4427130.1"
                     /translation="MIYSNQIKYSGKDGIYFHLRDNVDAFLTLSATENMEFDSPMQVT
                     TQNMQSGQTVTDNVQRAPRTITISGVVVVGYEGSLLLTRQGQLVENFIDTLESWRDQK
                     QIISVICKDGIKIDDSIITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDISAATG
                     KTATTNDGGVTSKKNAGNVTTERNNAPMLSCKELMNISDVSGYAPAIRNDILDAQIKC
                     NKAWKLDYSDLTEAEKTNVTNANSNAGRVASKLKKYSVNPNKRY"
     gene            complement(1631..3799)
                     /locus_tag="ACHKYO_RS01140"
                     /old_locus_tag="pgaptmp_000228"
     CDS             complement(1631..3799)
                     /locus_tag="ACHKYO_RS01140"
                     /old_locus_tag="pgaptmp_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4427131.1"
                     /translation="MAQFKVDDFLIELGFNSQRVLKGLEKAEKQTMQVASRIEKRLNK
                     AFKVNPTPLNDSLKVMERNVDKTVSKIEQRLKNTKAFKIKTEIEDTLKPLRQQRQPRI
                     SGNRAITAAYSANMSKLKGFDPILQKYIKSQFYGLSAKAGSMDSSKFNEKLAQLNASV
                     REAIAKARGHTSTSINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGLKRDS
                     AQRAAKFVLGDKASEAETFIRGLADKTGLNISEGLSSYAKFAAGAQGSMSQEQTQELF
                     GNATAMSRLMGLSNDELNGILKAFEQMASKGKIQAEELRGQLGDRMAGAFKLFAEALG
                     MTATELDKAMKDGKILSSDTLPKVAKQMGLMIDKAGGWAEVAKSTQTALGKLANNWDD
                     TMVKIFSGSQDELNGFLSRLSSLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRIFNNH
                     LEGWILQTKKLYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVMNLTR
                     AITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASMIPQI
                     RGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINEGWGR
                     IFSSMGELFNNATMNIARFNHPELITMKQDASLTSADIQGLRDEISALSKRIQEPVKV
                     SLGGEVAIKPDETSFMTFSSNIYDQYAEATLLSSSFPEDD"
     gene            complement(3913..4122)
                     /locus_tag="ACHKYO_RS01145"
                     /old_locus_tag="pgaptmp_000229"
     CDS             complement(3913..4122)
                     /locus_tag="ACHKYO_RS01145"
                     /old_locus_tag="pgaptmp_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4427132.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKSKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(4144..4851)
                     /locus_tag="ACHKYO_RS01150"
                     /old_locus_tag="pgaptmp_000230"
     CDS             complement(4144..4851)
                     /locus_tag="ACHKYO_RS01150"
                     /old_locus_tag="pgaptmp_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4427133.1"
                     /translation="MENLYINGKKIDSFGDWSPQTEHPAIAIQRKEHDERIALEQELK
                     MTPKEITFVSPEPQEMPDVCKSEALLELEKKYFPALKAQRLRLDDAYSKVTLMQSAIE
                     PSEFEIQDELSQKPFVYYQYADNDGFGTFPENIPDVINNLPDGYRIVKMVKASRGAGQ
                     FVYMSNKSDEELAELARQNILASRNKQLENVKLYLSRELQAMKELVSEYESQKKIAMQ
                     ADIEQLVKISTKYAKVL"
     gene            complement(5346..5654)
                     /locus_tag="ACHKYO_RS01155"
                     /old_locus_tag="pgaptmp_000231"
     CDS             complement(5346..5654)
                     /locus_tag="ACHKYO_RS01155"
                     /old_locus_tag="pgaptmp_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4427134.1"
                     /translation="MNNYPMTVLIDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHT
                     INEIKLGDFVHKLEVTDPDYKRFITEECNRLDLSPEQLLKLNIFLTSQSELNPYSEVM
                     "
     gene            complement(5811..6098)
                     /locus_tag="ACHKYO_RS01160"
                     /old_locus_tag="pgaptmp_000232"
     CDS             complement(5811..6098)
                     /locus_tag="ACHKYO_RS01160"
                     /old_locus_tag="pgaptmp_000232"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4427135.1"
                     /translation="MKPWHEDVRRYFAEHLIYDESSDSLCWSDGESVTINTDDYGNKT
                     FNIGRYTFYVKYVVWFLYHGYQSNKQIIHRNGNRADTRPKNLMQVRDFKRN"
     gene            complement(6095..6310)
                     /locus_tag="ACHKYO_RS01165"
                     /old_locus_tag="pgaptmp_000233"
     CDS             complement(6095..6310)
                     /locus_tag="ACHKYO_RS01165"
                     /old_locus_tag="pgaptmp_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4427136.1"
                     /translation="MLCITFEYHTDKMIRYISDLLIKGNGFGDIHNSKDIFIKAIGPN
                     ETLKTAVKPEWFERHKIELGYWGEEVL"
     gene            complement(6310..6588)
                     /locus_tag="ACHKYO_RS01170"
                     /old_locus_tag="pgaptmp_000234"
     CDS             complement(6310..6588)
                     /locus_tag="ACHKYO_RS01170"
                     /old_locus_tag="pgaptmp_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4427137.1"
                     /translation="MNKHTLTRISEEAFHELTRIKQTTNLPHQTIMQLAIAKEVTESD
                     LLKYPVSKGRRHIRITKNALATLKELNNFKIDIARLLSIKIHKLSMGV"
     gene            complement(6590..7141)
                     /locus_tag="ACHKYO_RS01175"
                     /old_locus_tag="pgaptmp_000235"
     CDS             complement(6590..7141)
                     /locus_tag="ACHKYO_RS01175"
                     /old_locus_tag="pgaptmp_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4427138.1"
                     /translation="MNDREFQKFLEESKRRNRHNGYSYTNNPTSYEVPAFSKTERKNI
                     EAVIRSITPRDRFMSVRKTTKNNLKPFLMSFDSYEQLPAKIEDLIIGTCRSFGRDNYH
                     RKVFYLLRNIDKISSSTITNYLQRQATRLSYELPSDKYCANLTTICMKVIETINHHVE
                     VGNISLTTSEPDFEFDPYILEGF"
     gene            7439..7627
                     /locus_tag="ACHKYO_RS01180"
                     /old_locus_tag="pgaptmp_000236"
     CDS             7439..7627
                     /locus_tag="ACHKYO_RS01180"
                     /old_locus_tag="pgaptmp_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4427139.1"
                     /translation="MVNLPPELGSPDVWAGGGIGLIAGLLLRYFIVRVFIVFRLFGRE
                     WSFDLKDRTRTDNHADPR"
     gene            8915..9484
                     /locus_tag="ACHKYO_RS01185"
                     /old_locus_tag="pgaptmp_000237"
     CDS             8915..9484
                     /locus_tag="ACHKYO_RS01185"
                     /old_locus_tag="pgaptmp_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4427140.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVKPSRVIEETISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAVNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            10082..10378
                     /gene="yciI"
                     /locus_tag="ACHKYO_RS01190"
                     /old_locus_tag="pgaptmp_000238"
     CDS             10082..10378
                     /gene="yciI"
                     /locus_tag="ACHKYO_RS01190"
                     /old_locus_tag="pgaptmp_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HGU4427141.1"
                     /translation="MLYVIYAQDKADSFEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10677..11930
                     /gene="kch"
                     /locus_tag="ACHKYO_RS01195"
                     /old_locus_tag="pgaptmp_000239"
     CDS             10677..11930
                     /gene="kch"
                     /locus_tag="ACHKYO_RS01195"
                     /old_locus_tag="pgaptmp_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HGU4427142.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(11985..12158)
                     /gene="yciY"
                     /locus_tag="ACHKYO_RS01200"
                     /old_locus_tag="pgaptmp_000240"
     CDS             complement(11985..12158)
                     /gene="yciY"
                     /locus_tag="ACHKYO_RS01200"
                     /old_locus_tag="pgaptmp_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HGU4427143.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            12301..13761
                     /gene="cls"
                     /locus_tag="ACHKYO_RS01205"
                     /old_locus_tag="pgaptmp_000241"
     CDS             12301..13761
                     /gene="cls"
                     /locus_tag="ACHKYO_RS01205"
                     /old_locus_tag="pgaptmp_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGU4427144.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            13796..14125
                     /gene="yciU"
                     /locus_tag="ACHKYO_RS01210"
                     /old_locus_tag="pgaptmp_000242"
     CDS             13796..14125
                     /gene="yciU"
                     /locus_tag="ACHKYO_RS01210"
                     /old_locus_tag="pgaptmp_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HGU4427145.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(14178..15182)
                     /gene="oppF"
                     /locus_tag="ACHKYO_RS01215"
                     /old_locus_tag="pgaptmp_000243"
     CDS             complement(14178..15182)
                     /gene="oppF"
                     /locus_tag="ACHKYO_RS01215"
                     /old_locus_tag="pgaptmp_000243"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HGU4427146.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(15179..16192)
                     /gene="oppD"
                     /locus_tag="ACHKYO_RS01220"
                     /old_locus_tag="pgaptmp_000244"
     CDS             complement(15179..16192)
                     /gene="oppD"
                     /locus_tag="ACHKYO_RS01220"
                     /old_locus_tag="pgaptmp_000244"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HGU4427147.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(16204..17112)
                     /gene="oppC"
                     /locus_tag="ACHKYO_RS01225"
                     /old_locus_tag="pgaptmp_000245"
     CDS             complement(16204..17112)
                     /gene="oppC"
                     /locus_tag="ACHKYO_RS01225"
                     /old_locus_tag="pgaptmp_000245"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGU4427148.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(17127..18047)
                     /gene="oppB"
                     /locus_tag="ACHKYO_RS01230"
                     /old_locus_tag="pgaptmp_000246"
     CDS             complement(17127..18047)
                     /gene="oppB"
                     /locus_tag="ACHKYO_RS01230"
                     /old_locus_tag="pgaptmp_000246"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HGU4427149.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(18133..19764)
                     /gene="oppA"
                     /locus_tag="ACHKYO_RS01235"
                     /old_locus_tag="pgaptmp_000247"
     CDS             complement(18133..19764)
                     /gene="oppA"
                     /locus_tag="ACHKYO_RS01235"
                     /old_locus_tag="pgaptmp_000247"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HGU4427150.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            20048..20344
                     /locus_tag="ACHKYO_RS01240"
                     /old_locus_tag="pgaptmp_000248"
     CDS             20048..20344
                     /locus_tag="ACHKYO_RS01240"
                     /old_locus_tag="pgaptmp_000248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4427151.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(20502..21149)
                     /gene="ychE"
                     /locus_tag="ACHKYO_RS01245"
                     /old_locus_tag="pgaptmp_000249"
     CDS             complement(20502..21149)
                     /gene="ychE"
                     /locus_tag="ACHKYO_RS01245"
                     /old_locus_tag="pgaptmp_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HGU4427152.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(21180..21233)
                     /gene="ychE"
                     /locus_tag="ACHKYO_RS01250"
                     /old_locus_tag="pgaptmp_000250"
     CDS             complement(21180..21233)
                     /gene="ychE"
                     /locus_tag="ACHKYO_RS01250"
                     /old_locus_tag="pgaptmp_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HGU4427153.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            21626..24301
                     /gene="adhE"
                     /locus_tag="ACHKYO_RS01255"
                     /old_locus_tag="pgaptmp_000251"
                     /gene_synonym="adhC"
     CDS             21626..24301
                     /gene="adhE"
                     /locus_tag="ACHKYO_RS01255"
                     /old_locus_tag="pgaptmp_000251"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGU4427154.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            24427..25755
                     /gene="insG"
                     /locus_tag="ACHKYO_RS01260"
                     /old_locus_tag="pgaptmp_000252"
     CDS             24427..25755
                     /gene="insG"
                     /locus_tag="ACHKYO_RS01260"
                     /old_locus_tag="pgaptmp_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HGU4427155.1"
                     /translation="MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTL
                     RKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSE
                     AVRRVFTKTAQLWHNATPHPQWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPAL
                     YPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLL
                     NAWSLAGEHRHWMIPLRKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGRGNEVTARL
                     LTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSK
                     KPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGAS
                     PGRIPELMHDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(26041..26658)
                     /gene="tdk"
                     /locus_tag="ACHKYO_RS01265"
                     /old_locus_tag="pgaptmp_000253"
     CDS             complement(26041..26658)
                     /gene="tdk"
                     /locus_tag="ACHKYO_RS01265"
                     /old_locus_tag="pgaptmp_000253"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGU4427156.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            27263..27676
                     /gene="hns"
                     /locus_tag="ACHKYO_RS01270"
                     /old_locus_tag="pgaptmp_000254"
     CDS             27263..27676
                     /gene="hns"
                     /locus_tag="ACHKYO_RS01270"
                     /old_locus_tag="pgaptmp_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HGU4427157.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(27820..28728)
                     /gene="galU"
                     /locus_tag="ACHKYO_RS01275"
                     /old_locus_tag="pgaptmp_000255"
     CDS             complement(27820..28728)
                     /gene="galU"
                     /locus_tag="ACHKYO_RS01275"
                     /old_locus_tag="pgaptmp_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGU4427158.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(28930..29943)
                     /gene="rssB"
                     /locus_tag="ACHKYO_RS01280"
                     /old_locus_tag="pgaptmp_000256"
     CDS             complement(28930..29943)
                     /gene="rssB"
                     /locus_tag="ACHKYO_RS01280"
                     /old_locus_tag="pgaptmp_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HGU4427159.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(30035..30940)
                     /gene="rssA"
                     /locus_tag="ACHKYO_RS01285"
                     /old_locus_tag="pgaptmp_000257"
     CDS             complement(30035..30940)
                     /gene="rssA"
                     /locus_tag="ACHKYO_RS01285"
                     /old_locus_tag="pgaptmp_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HGU4427160.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            31053..31511
                     /gene="ychJ"
                     /locus_tag="ACHKYO_RS01290"
                     /old_locus_tag="pgaptmp_000258"
     CDS             31053..31511
                     /gene="ychJ"
                     /locus_tag="ACHKYO_RS01290"
                     /old_locus_tag="pgaptmp_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HGU4427161.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFENCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            31561..32403
                     /gene="purU"
                     /locus_tag="ACHKYO_RS01295"
                     /old_locus_tag="pgaptmp_000259"
     CDS             31561..32403
                     /gene="purU"
                     /locus_tag="ACHKYO_RS01295"
                     /old_locus_tag="pgaptmp_000259"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HGU4427162.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            32563..32647
                     /locus_tag="ACHKYO_RS01300"
                     /old_locus_tag="pgaptmp_000260"
     tRNA            32563..32647
                     /locus_tag="ACHKYO_RS01300"
                     /old_locus_tag="pgaptmp_000260"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32597..32599,aa:Tyr,seq:gta)
     gene            32682..32766
                     /locus_tag="ACHKYO_RS01305"
                     /old_locus_tag="pgaptmp_000261"
     tRNA            32682..32766
                     /locus_tag="ACHKYO_RS01305"
                     /old_locus_tag="pgaptmp_000261"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32716..32718,aa:Tyr,seq:gta)
     gene            32814..32945
                     /locus_tag="ACHKYO_RS01310"
                     /old_locus_tag="pgaptmp_000262"
     ncRNA           32814..32945
                     /ncRNA_class="other"
                     /locus_tag="ACHKYO_RS01310"
                     /old_locus_tag="pgaptmp_000262"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(33129..33806)
                     /gene="narI"
                     /locus_tag="ACHKYO_RS01315"
                     /old_locus_tag="pgaptmp_000263"
     CDS             complement(33129..33806)
                     /gene="narI"
                     /locus_tag="ACHKYO_RS01315"
                     /old_locus_tag="pgaptmp_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU4427163.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(33806..34516)
                     /gene="narJ"
                     /locus_tag="ACHKYO_RS01320"
                     /old_locus_tag="pgaptmp_000264"
     CDS             complement(33806..34516)
                     /gene="narJ"
                     /locus_tag="ACHKYO_RS01320"
                     /old_locus_tag="pgaptmp_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU4427164.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(34513..36051)
                     /gene="narH"
                     /locus_tag="ACHKYO_RS01325"
                     /old_locus_tag="pgaptmp_000265"
     CDS             complement(34513..36051)
                     /gene="narH"
                     /locus_tag="ACHKYO_RS01325"
                     /old_locus_tag="pgaptmp_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU4427165.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(36048..39791)
                     /gene="narG"
                     /locus_tag="ACHKYO_RS01330"
                     /old_locus_tag="pgaptmp_000266"
     CDS             complement(36048..39791)
                     /gene="narG"
                     /locus_tag="ACHKYO_RS01330"
                     /old_locus_tag="pgaptmp_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HGU4427166.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVESLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(40184..41575)
                     /gene="narK"
                     /locus_tag="ACHKYO_RS01335"
                     /old_locus_tag="pgaptmp_000267"
     CDS             complement(40184..41575)
                     /gene="narK"
                     /locus_tag="ACHKYO_RS01335"
                     /old_locus_tag="pgaptmp_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HGU4427167.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWTVYGRHSKK"
     gene            41914..43710
                     /gene="narX"
                     /locus_tag="ACHKYO_RS01340"
                     /old_locus_tag="pgaptmp_000268"
     CDS             41914..43710
                     /gene="narX"
                     /locus_tag="ACHKYO_RS01340"
                     /old_locus_tag="pgaptmp_000268"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HGU4427168.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            43703..44353
                     /gene="narL"
                     /locus_tag="ACHKYO_RS01345"
                     /old_locus_tag="pgaptmp_000269"
     CDS             43703..44353
                     /gene="narL"
                     /locus_tag="ACHKYO_RS01345"
                     /old_locus_tag="pgaptmp_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HGU4427169.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(44354..45748)
                     /gene="ychO"
                     /locus_tag="ACHKYO_RS01350"
                     /old_locus_tag="pgaptmp_000270"
                     /gene_synonym="iatA"
     CDS             complement(44354..45748)
                     /gene="ychO"
                     /locus_tag="ACHKYO_RS01350"
                     /old_locus_tag="pgaptmp_000270"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HGU4427170.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            45933..46286
                     /gene="ychN"
                     /locus_tag="ACHKYO_RS01355"
                     /old_locus_tag="pgaptmp_000271"
     CDS             45933..46286
                     /gene="ychN"
                     /locus_tag="ACHKYO_RS01355"
                     /old_locus_tag="pgaptmp_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HGU4427171.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(46330..47025)
                     /gene="chaC"
                     /locus_tag="ACHKYO_RS01360"
                     /old_locus_tag="pgaptmp_000272"
     CDS             complement(46330..47025)
                     /gene="chaC"
                     /locus_tag="ACHKYO_RS01360"
                     /old_locus_tag="pgaptmp_000272"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HGU4427172.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(47183..47413)
                     /gene="chaB"
                     /locus_tag="ACHKYO_RS01365"
                     /old_locus_tag="pgaptmp_000273"
     CDS             complement(47183..47413)
                     /gene="chaB"
                     /locus_tag="ACHKYO_RS01365"
                     /old_locus_tag="pgaptmp_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HGU4427173.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            47683..48783
                     /gene="chaA"
                     /locus_tag="ACHKYO_RS01370"
                     /old_locus_tag="pgaptmp_000274"
     CDS             47683..48783
                     /gene="chaA"
                     /locus_tag="ACHKYO_RS01370"
                     /old_locus_tag="pgaptmp_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HGU4427174.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            49188..49295
                     /locus_tag="ACHKYO_RS01375"
                     /old_locus_tag="pgaptmp_000275"
     CDS             49188..49295
                     /locus_tag="ACHKYO_RS01375"
                     /old_locus_tag="pgaptmp_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4427175.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            49724..49831
                     /locus_tag="ACHKYO_RS01380"
                     /old_locus_tag="pgaptmp_000276"
     CDS             49724..49831
                     /locus_tag="ACHKYO_RS01380"
                     /old_locus_tag="pgaptmp_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4427176.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            50259..50366
                     /locus_tag="ACHKYO_RS01385"
                     /old_locus_tag="pgaptmp_000277"
     CDS             50259..50366
                     /locus_tag="ACHKYO_RS01385"
                     /old_locus_tag="pgaptmp_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4427177.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(50515..51369)
                     /gene="kdsA"
                     /locus_tag="ACHKYO_RS01390"
                     /old_locus_tag="pgaptmp_000278"
     CDS             complement(50515..51369)
                     /gene="kdsA"
                     /locus_tag="ACHKYO_RS01390"
                     /old_locus_tag="pgaptmp_000278"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HGU4427178.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(51405..52214)
                     /gene="sirB1"
                     /locus_tag="ACHKYO_RS01395"
                     /old_locus_tag="pgaptmp_000279"
     CDS             complement(51405..52214)
                     /gene="sirB1"
                     /locus_tag="ACHKYO_RS01395"
                     /old_locus_tag="pgaptmp_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HGU4427179.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQQEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(52218..52610)
                     /gene="sirB2"
                     /locus_tag="ACHKYO_RS01400"
                     /old_locus_tag="pgaptmp_000280"
     CDS             complement(52218..52610)
                     /gene="sirB2"
                     /locus_tag="ACHKYO_RS01400"
                     /old_locus_tag="pgaptmp_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HGU4427180.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(52607..53440)
                     /gene="prmC"
                     /locus_tag="ACHKYO_RS01405"
                     /old_locus_tag="pgaptmp_000281"
     CDS             complement(52607..53440)
                     /gene="prmC"
                     /locus_tag="ACHKYO_RS01405"
                     /old_locus_tag="pgaptmp_000281"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU4427181.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(53440..54522)
                     /gene="prfA"
                     /locus_tag="ACHKYO_RS01410"
                     /old_locus_tag="pgaptmp_000282"
     CDS             complement(53440..54522)
                     /gene="prfA"
                     /locus_tag="ACHKYO_RS01410"
                     /old_locus_tag="pgaptmp_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGU4427182.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(54564..55820)
                     /gene="hemA"
                     /locus_tag="ACHKYO_RS01415"
                     /old_locus_tag="pgaptmp_000283"
     CDS             complement(54564..55820)
                     /gene="hemA"
                     /locus_tag="ACHKYO_RS01415"
                     /old_locus_tag="pgaptmp_000283"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HGU4427183.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            56034..56657
                     /gene="lolB"
                     /locus_tag="ACHKYO_RS01420"
                     /old_locus_tag="pgaptmp_000284"
     CDS             56034..56657
                     /gene="lolB"
                     /locus_tag="ACHKYO_RS01420"
                     /old_locus_tag="pgaptmp_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HGU4427184.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            56657..57508
                     /gene="ispE"
                     /locus_tag="ACHKYO_RS01425"
                     /old_locus_tag="pgaptmp_000285"
     CDS             56657..57508
                     /gene="ispE"
                     /locus_tag="ACHKYO_RS01425"
                     /old_locus_tag="pgaptmp_000285"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HGU4427185.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLIKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            57659..58606
                     /gene="prs"
                     /locus_tag="ACHKYO_RS01430"
                     /old_locus_tag="pgaptmp_000286"
     CDS             57659..58606
                     /gene="prs"
                     /locus_tag="ACHKYO_RS01430"
                     /old_locus_tag="pgaptmp_000286"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGU4427186.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            58731..60410
                     /gene="dauA"
                     /locus_tag="ACHKYO_RS01435"
                     /old_locus_tag="pgaptmp_000287"
     CDS             58731..60410
                     /gene="dauA"
                     /locus_tag="ACHKYO_RS01435"
                     /old_locus_tag="pgaptmp_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HGU4427187.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(60465..60743)
                     /gene="ychH"
                     /locus_tag="ACHKYO_RS01440"
                     /old_locus_tag="pgaptmp_000288"
     CDS             complement(60465..60743)
                     /gene="ychH"
                     /locus_tag="ACHKYO_RS01440"
                     /old_locus_tag="pgaptmp_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HGU4427188.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            61021..61605
                     /gene="pth"
                     /locus_tag="ACHKYO_RS01445"
                     /old_locus_tag="pgaptmp_000289"
     CDS             61021..61605
                     /gene="pth"
                     /locus_tag="ACHKYO_RS01445"
                     /old_locus_tag="pgaptmp_000289"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGU4427189.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            61722..62813
                     /gene="ychF"
                     /locus_tag="ACHKYO_RS01450"
                     /old_locus_tag="pgaptmp_000290"
     CDS             61722..62813
                     /gene="ychF"
                     /locus_tag="ACHKYO_RS01450"
                     /old_locus_tag="pgaptmp_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGU4427190.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            63654..66521
                     /gene="ycgV"
                     /locus_tag="ACHKYO_RS01455"
                     /old_locus_tag="pgaptmp_000291"
     CDS             63654..66521
                     /gene="ycgV"
                     /locus_tag="ACHKYO_RS01455"
                     /old_locus_tag="pgaptmp_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4427191.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVLDSQNNG
                     VKANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
     gene            complement(66621..68540)
                     /gene="dhaR"
                     /locus_tag="ACHKYO_RS01460"
                     /old_locus_tag="pgaptmp_000292"
     CDS             complement(66621..68540)
                     /gene="dhaR"
                     /locus_tag="ACHKYO_RS01460"
                     /old_locus_tag="pgaptmp_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HGU4427192.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            68768..69838
                     /gene="dhaK"
                     /locus_tag="ACHKYO_RS01465"
                     /old_locus_tag="pgaptmp_000293"
     CDS             68768..69838
                     /gene="dhaK"
                     /locus_tag="ACHKYO_RS01465"
                     /old_locus_tag="pgaptmp_000293"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HGU4427193.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLMI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLVNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            69849..70481
                     /gene="dhaL"
                     /locus_tag="ACHKYO_RS01470"
                     /old_locus_tag="pgaptmp_000294"
     CDS             69849..70481
                     /gene="dhaL"
                     /locus_tag="ACHKYO_RS01470"
                     /old_locus_tag="pgaptmp_000294"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HGU4427194.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            70492..71910
                     /gene="dhaM"
                     /locus_tag="ACHKYO_RS01475"
                     /old_locus_tag="pgaptmp_000295"
     CDS             70492..71910
                     /gene="dhaM"
                     /locus_tag="ACHKYO_RS01475"
                     /old_locus_tag="pgaptmp_000295"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HGU4427195.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICKQGEKL
                     YAIQPEETLTLDVKRQRFNRQG"
     gene            72230..73927
                     /gene="treA"
                     /locus_tag="ACHKYO_RS01480"
                     /old_locus_tag="pgaptmp_000296"
     CDS             72230..73927
                     /gene="treA"
                     /locus_tag="ACHKYO_RS01480"
                     /old_locus_tag="pgaptmp_000296"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU4427196.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GSLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFAMMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(74006..74446)
                     /gene="ycgY"
                     /locus_tag="ACHKYO_RS01485"
                     /old_locus_tag="pgaptmp_000297"
     CDS             complement(74006..74446)
                     /gene="ycgY"
                     /locus_tag="ACHKYO_RS01485"
                     /old_locus_tag="pgaptmp_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HGU4427197.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSCRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRYKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(74624..74878)
                     /gene="ymgE"
                     /locus_tag="ACHKYO_RS01490"
                     /old_locus_tag="pgaptmp_000298"
     CDS             complement(74624..74878)
                     /gene="ymgE"
                     /locus_tag="ACHKYO_RS01490"
                     /old_locus_tag="pgaptmp_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU4427198.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            75079..75813
                     /gene="ycgR"
                     /locus_tag="ACHKYO_RS01495"
                     /old_locus_tag="pgaptmp_000299"
     CDS             75079..75813
                     /gene="ycgR"
                     /locus_tag="ACHKYO_RS01495"
                     /old_locus_tag="pgaptmp_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HGU4427199.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMRQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(75815..76426)
                     /gene="emtA"
                     /locus_tag="ACHKYO_RS01500"
                     /old_locus_tag="pgaptmp_000300"
     CDS             complement(75815..76426)
                     /gene="emtA"
                     /locus_tag="ACHKYO_RS01500"
                     /old_locus_tag="pgaptmp_000300"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HGU4427200.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            76526..77440
                     /gene="ldcA"
                     /locus_tag="ACHKYO_RS01505"
                     /old_locus_tag="pgaptmp_000301"
     CDS             76526..77440
                     /gene="ldcA"
                     /locus_tag="ACHKYO_RS01505"
                     /old_locus_tag="pgaptmp_000301"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HGU4427201.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            77535..79271
                     /gene="cvrA"
                     /locus_tag="ACHKYO_RS01510"
                     /old_locus_tag="pgaptmp_000302"
     CDS             77535..79271
                     /gene="cvrA"
                     /locus_tag="ACHKYO_RS01510"
                     /old_locus_tag="pgaptmp_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HGU4427202.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(79657..80727)
                     /gene="dadX"
                     /locus_tag="ACHKYO_RS01515"
                     /old_locus_tag="pgaptmp_000303"
     CDS             complement(79657..80727)
                     /gene="dadX"
                     /locus_tag="ACHKYO_RS01515"
                     /old_locus_tag="pgaptmp_000303"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HGU4427203.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(80737..82035)
                     /gene="dadA"
                     /locus_tag="ACHKYO_RS01520"
                     /old_locus_tag="pgaptmp_000304"
     CDS             complement(80737..82035)
                     /gene="dadA"
                     /locus_tag="ACHKYO_RS01520"
                     /old_locus_tag="pgaptmp_000304"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HGU4427204.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            82365..83897
                     /gene="ycgB"
                     /locus_tag="ACHKYO_RS01525"
                     /old_locus_tag="pgaptmp_000305"
     CDS             82365..83897
                     /gene="ycgB"
                     /locus_tag="ACHKYO_RS01525"
                     /old_locus_tag="pgaptmp_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HGU4427205.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(83949..84668)
                     /gene="fadR"
                     /locus_tag="ACHKYO_RS01530"
                     /old_locus_tag="pgaptmp_000306"
     CDS             complement(83949..84668)
                     /gene="fadR"
                     /locus_tag="ACHKYO_RS01530"
                     /old_locus_tag="pgaptmp_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HGU4427206.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            84889..86430
                     /gene="nhaB"
                     /locus_tag="ACHKYO_RS01535"
                     /old_locus_tag="pgaptmp_000307"
     CDS             84889..86430
                     /gene="nhaB"
                     /locus_tag="ACHKYO_RS01535"
                     /old_locus_tag="pgaptmp_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HGU4427207.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHIREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            86576..87106
                     /gene="dsbB"
                     /locus_tag="ACHKYO_RS01540"
                     /old_locus_tag="pgaptmp_000308"
     CDS             86576..87106
                     /gene="dsbB"
                     /locus_tag="ACHKYO_RS01540"
                     /old_locus_tag="pgaptmp_000308"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HGU4427208.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(87152..88420)
                     /gene="umuC"
                     /locus_tag="ACHKYO_RS01545"
                     /old_locus_tag="pgaptmp_000309"
     CDS             complement(87152..88420)
                     /gene="umuC"
                     /locus_tag="ACHKYO_RS01545"
                     /old_locus_tag="pgaptmp_000309"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HGU4427209.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(88420..88839)
                     /gene="umuD"
                     /locus_tag="ACHKYO_RS01550"
                     /old_locus_tag="pgaptmp_000310"
     CDS             complement(88420..88839)
                     /gene="umuD"
                     /locus_tag="ACHKYO_RS01550"
                     /old_locus_tag="pgaptmp_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HGU4427210.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT        22687 a        22329 c        21239 g        22807 t
ORIGIN      
        1 aagtgataac ccattaacga ttaacatctt caagggatta ttccggtgtc agtgtggggc
       61 tagtgttcac cctacaggga ctaagaataa gtatcagggt gtttatcggt gcaataacca
      121 tcttgacggt cgctgtgatg ttccaccgtt gaagcgtaaa ccgtttgata agtggatgat
      181 tgataatttt ctggggatga ttgacgtggg gaatgatgga gaatcagaga ggaagattgc
      241 agcgttacag catgaggttg aaattgtcac agccagaatc aagaaagcta ccgccctact
      301 tcttgagatg gatgatatta cagagttgaa aacacaggtg aagggactta accagaagcg
      361 cacagagcta cagaccacga ttgataacat gaggcgtaag gcttcactca ctgataagga
      421 actaccccag cttaaagata ttgacctgac aactaaagcg ggtcgtgtcg agtgtcagtt
      481 gattttgtcc aaacatctaa aagggcttac attgggtaag gattcggtaa ctgtaacgct
      541 acagaacgac actgaaataa ccatacctac agacccgcta ccgctaaatg atggaacatc
      601 aatctttgaa attgctgaga aaaatctttt gaatattgac actttccaac tgtagcaatc
      661 aatcaggggg ctatgccccc ttctatcaat atctcttatt aggatttact gagtactttt
      721 taagctttga agctactcga ccagcgttac tgttagcatt ggtgacgttg gttttttcag
      781 cttctgttaa gtctgaatag tccagcttcc aagccttatt acatttaatc tgagcatcga
      841 gaatatcatt tctgatagct ggagcgtaac cggatacatc gctaatattc attaactctt
      901 tgcacgatag cataggcgcg ttattgcgtt cagtggtcac gttacctgca ttcttcttac
      961 tggtcacacc accgtcattt gtggtagcgg tttttcctgt cgcagccgaa atatcagtct
     1021 gacccacaat ggctttgaag ttaatctcct gaaaagttaa ctggattctc agaccattcg
     1081 aaataccaac atctttagag gctttaaagc ttgtaataat ggaatcatca attttaatac
     1141 catctttaca gatgaccgaa ataatttgct tctggtcacg ccagctttcc agagtgtcga
     1201 tgaagttttc tactaattga ccctgacgag ttaataacaa gcttccttca tagccaacta
     1261 caacgacacc actaatagtg attgttctgg gtgctctttg cacattatct gtgacggttt
     1321 gccctgattg catgttctgt gtagttacct gcatagggct gtcaaattcc atgttctcag
     1381 ttgctgataa ggttaagaag gcatctacat tatctcttaa gtggaaatag atgccgtctt
     1441 tgccactata tttgatttgg ttagagtaaa tcattaaatt cccctttgat ttcaaagact
     1501 tagtgcattt taatcatttt taaccaagcc tttgatattt aagtgtttta tctgttaagc
     1561 gtgagctacg taaattctat ttttcagtgc gggtattaac tttgctttat tcacgtagaa
     1621 caccgttaaa tcaatcatct tctgggaatg atgaacttaa taacgtagct tctgcatatt
     1681 ggtcgtagat attactgcta aaggtcataa agctggtttc atctggttta atggcaactt
     1741 caccaccaag tgaaactttc acaggctcct gaatacgctt acttaaagca gagatttcat
     1801 ccctcagacc ctgaatatct gcactcgtta atgatgcatc ttgtttcatg gtaattaatt
     1861 ctggatgatt gaatcttgca atattcattg tggcattgtt aaacaattca cccattgaac
     1921 taaaaattct accccatcct tcgttaatca tattcggttt attagggtca ccagaataca
     1981 caggattaaa gtcactaccc acacctaccc cagacggatg catattagcg tttttgacta
     2041 cttcctgatt cataaaatcc agtgctttct tcaatgcata taaaccaaga gtgacgcctc
     2101 tgatttgtgg aatcattgaa gctaatgcta acgctgtttc ataaccgtca tcagcataac
     2161 caattgcaag agcactacct acaccaccag ctacaccttt cagttttcca ccacctttac
     2221 cagtagctac ctgaccagcc acctgattag ctcttgtacc taacgttgtg attgctcttg
     2281 tcaggttcat cacccctgta gtggcactga atagtgtctt tgcagataac gctatagcaa
     2341 gacctttgac aaaattgata aaaccgtcac cgacttcatc aagtagtttt cgtttagtgt
     2401 catcaaggct gtaatagagc ttcttagtct gtaaaatcca gccttcaagg tgattgttaa
     2461 agatacggat gtcatctaca ccagctttaa gcatgtcgat aagtccaccg atagcatcac
     2521 cagcgataga agaactcata cccatttcac taagcaggct actcaggcgg gataagaagc
     2581 cgtttaactc atcctgtgaa cctgagaaga ttttaaccat cgtatcatcc cagttgtttg
     2641 ctaacttacc taaagctgtc tgagtgctct tagcgacttc agcccaacca cccgctttat
     2701 caatcatcaa gcccatctgt ttagcgactt tagggagagt atcactactt aaaatcttcc
     2761 cgtctttcat cgctttatca agctctgtag cagtcatacc caatgcttca gcgaatagct
     2821 taaatgcacc agccatacgg tcacctaact gcccacgaag ctcttcagct tgaatcttgc
     2881 ccttgctcgc catctgctca aatgctttca gaatgccgtt tagctcgtcg ttagaaagcc
     2941 ccattaagcg actcatagcc gttgcattac caaatagctc ctgtgtctgt tcctgtgaca
     3001 tagaaccctg agcacctgct gcaaacttag cgtagcttga tagcccttca gagatattaa
     3061 gacctgtctt gtcagccagt cccctgataa atgtttcagc ctcagaggct ttatcaccaa
     3121 gcacaaactt agcggctctc tgtgcggaat ctcgtttaag acctgcattc ataatggcct
     3181 tgtaagcgtt aagagcaccc atcacagagt aaaatgcacc agataactta attgcattgc
     3241 ttgcaagatt attaaatgcg tcgcttgtat tgttaatgct ggtggatgta tgacctctgg
     3301 ctttagcaat tgcttctctg actgaagcgt ttaactgagc taacttctca ttgaattttg
     3361 aagaatccat tgacccagct ttagcagata gcccgtaaaa ctgagatttg atgtatttct
     3421 gcaaaatagg gtcaaagcct tttagcttag acatattagc tgaataagct gctgtgattg
     3481 ctctgttgcc gctaattcgt ggctgtcttt gttgtctaag tggctttaaa gtatcctcaa
     3541 tctcagtttt aatcttaaac gctttagtgt ttttaagacg ctgctcaatc ttggaaacag
     3601 tcttatcgac atttctttcc atgaccttga gagaatcatt taaaggcgta gggttaactt
     3661 taaaagcttt attcaatcgc ttctcaattc gtgatgctac ctgcatcgtt tgtttctcgg
     3721 ctttttccag accttttaaa accctctgag agttaaaacc tagctcaatc agaaaatcat
     3781 caactttaaa ttgtgccatt catatttttt cacctcatac aaaaaaggga gaaactaccg
     3841 atatcactcg ataatctctc cccgtattat agattcgata gaacctatct catataccca
     3901 accaattctt ttttaatgtc tgacaagata atgtaattcc gtttcaccta tgatttgagc
     3961 atcagaagga atacggtcaa tacaccactt tggaatcttg cgtgtttttg gtttgtgtga
     4021 ggtgataact tgacgtttgc ttttaattct taaggctggt ttagtttgta actttaagtt
     4081 ttcacaaaca gaccgaatag tctctaaacg ttcttcaaac attaatcacc tcttaattcg
     4141 taatcaaagc acctttgcgt acttcgttga aatcttcact aactgctcaa tatcagcttg
     4201 catagcaatc ttcttctgcg actcatattc actaacaagc tctttcatag cctgaagctc
     4261 tcttgagaga tacaacttaa cattttcaag ttgcttgtta cgtgatgcca agatgttctg
     4321 tctggcaagt tcagcgagtt cttcatcgct cttattactc atgtaaacaa actggccagc
     4381 acctcttgat gctttaacca tcttcacaat tcgatatcca tcaggaagat tattaatcac
     4441 gtcggggata ttctcaggaa aagtaccaaa accatcattg tctgcatatt ggtaataaac
     4501 aaatggtttc tgtgacaatt catcctgaat ctcaaattct gacggttcaa tagctgattg
     4561 catcagggta actttgctgt aagcgtcatc taatctaaga cgctgtgctt ttaaagctgg
     4621 gaaatatttc ttttcaagct caagcaatgc ttcactcttg catacatcag gcatctcttg
     4681 aggctcagga gacacaaagg taatttcctt tggtgtcatc tttaactctt gctcaagtgc
     4741 aattctttca tcgtgctctt tgcgctgaat agcgatagct ggatgttcag tctgtggtga
     4801 ccagtcaccg aagctatcaa tctttttacc gttaatatat agattttcca tttcattttc
     4861 ctcttctaag taggtattca gtttttaaaa agcacacctg acagccatac aaacttagaa
     4921 gagacttaaa aggcggtttg ccaagtatgc tttttaaaag ctgaaaaagc cctagataac
     4981 taaaacgcta taaagggcta aggtgaagaa attaaagaac atctaaagga ttcaatgaat
     5041 gaacaaagac ttttcgagat gacctctcag gttatataag aggtcatgta gcgaaaagca
     5101 ttgcaccttt cagaatgttc ggtatagaac atacctcacc aactcagaat aactatcatc
     5161 gatgtagaga tgattaatat tttaattcaa ggagaattgc tctctccata tatacaatta
     5221 tgtcatgctc aaggttattt gtcaacagct tttaacagat tgataacatc aatttctgtt
     5281 agcttcgcta gcaatcaggc aaccacataa taaacctaaa gctgtccata aaataacgag
     5341 tgtcattaca tcacctcgct gtaagggttt aattcactct gagaggtcaa gaatatattc
     5401 agcttcaata attgctcagg acttaaatca agtcgattgc actcttcagt gataaatctc
     5461 ttgtagtctg ggtctgttac ttccagtttg tgaacaaagt caccaagttt aatttcgttg
     5521 attgtatgct tgtcaatata cgttgatgct tcaaaacaat gcatgaaata gatattaaat
     5581 cctgacctat tcaggtcaat caaccaattc atagtttctt ggtcaattag tactgtcatt
     5641 gggtaattgt tcatttccta aaatctcctt tttaccagac ctcctcaatc gctctgtaac
     5701 gagctaaaca gtctttttca agggtagcct gtacagtggt attacccacc ctaaaataag
     5761 atagtttttt gcgttagaat gcgattgaga gcgtctgaga agctattttt ctaatttctc
     5821 ttaaagtctc ggacttgcat aagatttttt ggtctggtat cggctctgtt accatttcga
     5881 tggataattt gcttgttgga ttgatagcca tgataaagga accagacaac atatttcaca
     5941 tagaatgtgt atcgacctat attaaatgtt ttgttgccgt agtcgtcggt atttatcgtg
     6001 actgactccc catcagacca gcacagggaa tcactggatt catcatagat aaggtgttct
     6061 gcaaaatatc ttctaacatc ttcatgccaa ggtttcataa aacctcctca ccccaataac
     6121 ctaattcaat cttatgacgt tcaaaccatt ccggtttaac tgctgttttc agtgtttcat
     6181 ttgggccaat ggctttaatg aaaatatctt ttgagttatg gatgtcaccg aatccgtttc
     6241 ctttaatcag gaggtcggag atgtagcgaa tcattttatc tgtgtggtat tcaaaggtaa
     6301 tgcatagcat taaaccccca tcgatagctt gtgaatcttg atgctcaata gtcgggcaat
     6361 atcaatttta aagttgttca gttccttcag agtagccaga gcgttcttag taattctgat
     6421 gtgtctgcga cctttagaaa ctggatattt cagtaaatcc gactcagtga cctctttagc
     6481 aattgcaagc tgcataatag tttgatgagg caggttagtt gtctgtttaa ttcgggtaag
     6541 ctcgtgaaaa gcttcttctg aaatacgggt caaagtgtgt ttattcattt taaaatccct
     6601 ccaaaatata ggggtcaaat tcaaaatctg gttcactggt tgtaaggcta atattgccca
     6661 cttccacatg atgattgatt gtctcgataa ctttcatgca aatggtagtt aaattagcgc
     6721 aatatttatc agatggtagc tcgtagctaa gtctggttgc ttgacgctgt agataatttg
     6781 ttattgtgct tgagctaatc ttgtcaatat ttcgtagtaa atagaaaact ttccggtgat
     6841 agttatccct tccaaaactg cgacaagtac cgataattaa atcttcaatt tttgctggca
     6901 gttgctcgta gctgtcaaaa ctcatcaaga aaggtttcaa gttattcttt gttgttttgc
     6961 gtactgacat aaatctgtct cgtggcgtaa tgctacgaat gaccgcttca atgtttttac
     7021 gttcggtttt ggaaaaggct gggacttcgt agctggtggg gttgtttgtg tagctgtaac
     7081 cgttgtgtct gttgcgtctc ttggattctt caagaaactt ttgaaattct ctgtcgttca
     7141 ttcataagct ggcccttata attgttaatt ctctgttaac tcatatatac aatcctagca
     7201 ttttcaaagg ctaatgtcaa gtatttttta acattttatt aacatctgtg gtataatggg
     7261 tttatgggga gagtaaaata taaaggcgtg gctatgagaa tggtcacatg gttattgcat
     7321 tgcacacaga cctcttcaat cgctctgtaa cgagttaaat ggcctcttca ggtcaactca
     7381 taaggtcagg tactttttgt gtcagaatgc ggttgagggc gtctcacaag gaaatttaat
     7441 ggtaaatctc cctcctgaac tggggtcacc tgatgtatgg gctggtggag gtattggact
     7501 tattgcaggt ctgttacttc gctatttcat cgttcgggtg tttattgtgt tcagattgtt
     7561 tggtcgtgaa tggtcgtttg acctcaagga caggaccaga acagataacc acgcagaccc
     7621 ccgttaaact cccttcaaaa tcccctgtac gagatgtata gggggtatct ttagcatttt
     7681 gtgctatcta tcaaaaaata gcttgacaga accggaaagc tgtatctatc aagtattcac
     7741 ccctctaaca gtaatttcta aaagttctta tttttcaata agttgtaatc acccaatata
     7801 taaggctgtc cagtaccttc acagagaaac ttcagaagcc ttcttaatca atccactaag
     7861 cgagagagag cgataacccc agagagggaa gctcccttct taatcgacct cctaaccagt
     7921 ctttacagac attgttcaag gtaatcacaa tagttttctt ccctcggttt tccgtgttcc
     7981 tcggtttgtc tattgagggc tttgaaatgc cctgtaactt ccctcttaga catggagaga
     8041 aagaacatag tgaccccaag cgaagcgccc ctctaaaaag attttttagc tattttgctt
     8101 aaaaccatat caaacgatac attttattga tttaggtcaa agacaaaagc attgacaaaa
     8161 tggtaagatt gctcacatta ccttatttaa cagatttctt aagagacttt gaaacagcct
     8221 gtttaacagg tcactacctc tggtcttatc gttcccttct ttctaggtag ttatctgtta
     8281 agagtttttc agtcttactc ttacagtagc tgttaagata gaacctacct tcggtttcat
     8341 ggttctcttc ctcccgttaa atattccctt catagcagac taaataaaac ctgtgtagtt
     8401 cttggtagaa tctttcaagg taacttagct ctgagtttat gaagttgacc ttagtcaata
     8461 gttgcctagg tgctgctaag gcttccttag tgggaaagag ggatatagtg accataggaa
     8521 cccctcatgt tatacaggct tctatactgg ttaaaatcta tacaggcaat atccagcctc
     8581 taaatttttt ataaaaaatg actagcccct ctacatttcc cttccccgat aatcttcgaa
     8641 aatatctgca atcaatctgt agctgctaag tacccgtcac accagctatg tgaccccctt
     8701 tgcagaattt taaaatatct attgactttc tatgtgagtt gctataaagg catctatcta
     8761 ggtctttaac tgtataaata cacatgacaa caaacacccg ttaagtgtct atataaaaag
     8821 gtagctgtag ttatagacaa caattgtgat aattgattaa atgtctatag acaaacaaaa
     8881 cagacctttg ttggctatat agagagataa gaacatgcgt acattccttt atgctcgtgt
     8941 atcagttgat gacctcacca cacagaacca agtagaagct gttaaacgtg ctggttatga
     9001 agtcaaacct tcacgagtga ttgaggaaac cattagtggt gctaccccag ctatggacag
     9061 accacaattt gtaaagctgg tggataagct ggaagaaggt gattgtcttg tagtgaccaa
     9121 gattgaccgc ttaggaaggg ataacattga cgtacagaag accgtcacaa tgttacttgg
     9181 tatgggtgtt aagctggtct gtctagattt gcctgtccaa gacctttcaa gtgctgaagg
     9241 taaacttatt cttcaactat tcagtacctt tgcagagttt gaacttaacc gcatcagaga
     9301 gcgaaccaga gagggtttag aacgagctaa agcacaaggt aaggtgttag gtcgccctgt
     9361 agctgtgaac actacagagg ccgttttaga gcataaggca aagggattat cacaaagtca
     9421 ggttgcaaag cttctgaatc tctctgtaag aacggttagc cgtcactgga caaagacact
     9481 gtaaaaaaaa cttagtagca tcttcatagc ctccttcgtg ggggcttttt tgtgtctgta
     9541 acatatggtg accaactgca tagatagcgt tatatgctcc tgtatatagc acttttctag
     9601 gagtcgagag agggacgact aacacgatac agtttaccct gaaaagagac ttcacagatt
     9661 ctctatatat ttcaataagt taggataaaa agagactata ccgatactgc atagaatctg
     9721 tgtaaaactg cttagatact tggaaatcta ttattagatt gcatagtcat aggggttgga
     9781 acaagcccaa ctacacctga taaagcctgt taataacagg tctcttagaa aaccgaatag
     9841 acttcattca agggtcattg aagtcataat catttcagta gaaaaaccag gtctcgattt
     9901 taaatgcaaa tagcaatcat attcaataag gcaagacgat caggatcagt gaaaatgtca
     9961 ttttgggcgt aattgacctt actttatgca tggtcttaac aaagacatca tctttccgtt
    10021 gcagatcttc gccctttcaa ataacgtact ttacaacttt cccgaacaag gagttgtgcc
    10081 cgtgttgtat gttatttacg ctcaagacaa agctgatagc ttcgaaaaac gcctttccgt
    10141 tcgtccggca catttagcac gtttacagtt actgcatgat gaaggtcgtt tgttgacagc
    10201 tggtccaatg ccagcggtag acagtaacga tccaggggct gcgggattta ctggctcaac
    10261 cgttattgct gaatttgaat cgctggaagc agctcaggcc tgggccgatg ctgacccgta
    10321 cgtagcggca ggagtctatg aacacgtttc agtgaaacca tttaagaaag tgttctgaat
    10381 agagattcaa tatttaaggg cacggttttt gcaaccgtgt tcttgtttgg cctttcctta
    10441 tagccggtca gtgcattcac caccacaagc agataataac tttatctgta tttctttata
    10501 tgatgtgaac atattcagga aaatgccctt tcctgacgca gagttgctcg ctcaaaacct
    10561 ttgattactg ccgataaagt catctactat tgtttaacac attcacgcgc tgcatgtatg
    10621 ttcagaaaat gtgcacgcta agataattaa acaaaattgt ggttacagag gtatcagtga
    10681 gtcactgggc tacattcaaa caaacagcaa caaatttatg ggtgacccta cggcacgaca
    10741 ttctcgcgct ggccgtcttt ttaaatggat tgcttatttt taaaacaatc tatggtatgt
    10801 cggtcaattt gcttgatatt ttccatatca aagcattttc agagttggat ctctccttgc
    10861 tggcaaacgc cccactattt atgctcggcg tctttcttgt cctgaactcc attggcttac
    10921 tgttccgggc aaagctcgca tgggcaatca gtatcatttt gttgttgata gcgctaattt
    10981 acaccctgca tttttatccc tggctgaaat ttagtattgg attttgcatt tttacgctgg
    11041 tgtttttgct gatactgcgc aaagacttct cccacagtag cgccgcagcc gggacaattt
    11101 ttgcatttat tagtttcacg acattactgt tttactcaac ctacggtgcg ctttatttaa
    11161 gcgaaggttt taatccgcga atagaaagtt tgatgaccgc gttctatttt tcgatagaaa
    11221 ccatgtcaac cgtcggctat ggcgatattg tccctgtttc tgaatcagca cgattgttca
    11281 ctatttcggt cattatttcc ggcattaccg tttttgccac atctatgacc tcaatttttg
    11341 gtccgcttat ccgcggggga ttcaacaaac ttgtaaaagg aaacaatcat acaatgcatc
    11401 gtaaagatca ttttattgtt tgcggacatt cgattctcgc catcaatacg atcctgcaac
    11461 tgaatcaacg cggacaaaac gtaacggtta tcagcaactt gcctgaagat gatatcaagc
    11521 aacttgagca acgcttaggc gataacgctg atgttatccc cggtgacagt aatgacagtt
    11581 cagtattaaa gaaagcggga atcgatcgtt gccgggccat tctggcgctg agtgataacg
    11641 atgcagataa cgcgtttgtt gtactctcgg caaaagatat gagcagtgat gtcaaaacag
    11701 ttctcgccgt cagtgatagc aaaaacctga ataagattaa gatggtacat ccggatatca
    11761 ttctctcacc gcaactgttt ggcagcgaaa ttctggcgcg agttttaaat ggtgaagaga
    11821 ttaataatga tatgctcgtt tcaatgttgt tgaactccgg tcatggcatt ttcagcgata
    11881 acgatgaaca agaaacgaaa gctgacagta aagaatcagt gcaaaaatag agtttaaaga
    11941 cgaaagagta aacttattgg gcaccgcaat ggtgcccagt atatttagat attgtagatc
    12001 gcaaacaata acgagttacg ctgtttgttt agaatgcact tcctgatgct gtggatacgc
    12061 atatttcggc gcgattgccc ctcaagccaa cgcgatttac gtcggctagc ctgacgctgc
    12121 atgcgccagc gccccacttc cgttctactc cgcttcatgt taactactct atgcaataac
    12181 agaatggtca ttataccccc acccccgacg ctgaccagtg cttttcccgc gtttttattt
    12241 gccagatgaa tccatatgcg taaactcata acaatgcgct ttcaaaagga tttctaatct
    12301 atgacaaccg tttatacgtt ggtgagttgg ttggccattc tgggatactg gttgctcatt
    12361 gcaggcgtaa ctttacgcat tctaatgaaa cgacgcgcag ttccctccgc gatggcctgg
    12421 ctgttgatta tttacattct gccgttagtc ggaattattg cctatcttgc cgttggcgag
    12481 ctccatttag gcaaacgccg cgctgagcgc gccagagcga tgtggccttc caccgcaaaa
    12541 tggcttaacg accttaaagc ctgtaagcat atcttcgccg aagaaaatag cagtgtcgct
    12601 gcgccattat tcaagctttg cgagcgtcgt caggggatcg ctggggtcaa agggaatcag
    12661 ctacaactga tgaccgagtc agatgatgtg atgcaggcgt taatccgcga catccagctc
    12721 gcgcgccata atattgagat ggtgttttat atctggcagc ccggcggcat ggcggaccag
    12781 gtggctgaat cattaatggc ggctgcacgg cgtggtattc attgccgatt gatgctcgac
    12841 tccgccggga gtgtggcttt tttccgcagc ccgtggcccg agctaatgcg taatgccggt
    12901 attgaagtgg tcgaagcctt aaaggtcaat ctgatgcgtg tgtttttacg ccgtatggac
    12961 ctgcgccaac atcgcaagat gatcatgatc gataattaca tcgcgtacac cggcagcatg
    13021 aatatggtcg atcctcgcta cttcaaacaa gatgcgggcg tagggcaatg gattgatctg
    13081 atggcgcgta tggaaggccc catcgccacc gcgatgggga ttatttattc ctgcgactgg
    13141 gagattgaaa ccggaaaacg tattctgccg ccaccaccag atgtcaatat tatgccgttt
    13201 gaacaggcca gcggtcacac cattcacaca attgcttctg gccccggctt tccggaagat
    13261 ctcattcacc aggcattatt gactgcggct tattcggcgc gtgaatattt gatcatgacc
    13321 acgccctact ttgtgccaag cgatgattta cttcatgcga tttgcacggc ggcgcagcgc
    13381 ggggtggatg tcagtattat ccttccgcga aaaaatgact cgatgctggt cggctgggcc
    13441 agtcgcgcat tctttacgga acttcttgct gctggggtta aaatttatca gtttgaaggc
    13501 gggttactgc ataccaagag tgtgctggtc gatggcgaac taagtctggt tggcaccgtt
    13561 aaccttgata tgcggagtct gtggctgaat ttcgagatta ccctggcaat cgacgataaa
    13621 ggttttggtg cagaccttgc cgccgttcag gacgattata tttcgcgttc acgtctgctc
    13681 gatgcccgtt tatggctaaa acgtccatta tggcaacgtg tcgccgagcg actgttttac
    13741 ttcttcagtc cgttgctgta aaacgtcgcc aacagaggtt aaacaggtag tgactatgga
    13801 tatggatcta aacaatcgcc tgactgaaga tgaaacgctt gagcaggcct acgatatttt
    13861 tctcgaactg gctgccgaca atctcgatcc agctgatgtt ctgctgttca atcttcagtt
    13921 tgaagagcgc ggcggcgcgg agttattcga tccggcggaa gactggcagg aacatgttga
    13981 tttcgatttg aacccagact tttttgccga agtggtgatt ggtctggcgg acagtgaaga
    14041 tggtgaaatc aacgatgttt tcgcccgcat tctgttatgc cgcgaaaaag atcacaaact
    14101 ttgccatatc atctggcggg aataaataat cgcttttagc gtgaaaaagg gctgacaact
    14161 gtcagccctt attgtgttta aagcggatcg actttcaggc aagaaacggc gtgtctgaaa
    14221 ctcccctcca gaacaggacg tgttttggcg cactccggac cggcaatcgg gcaacgggta
    14281 cggaaaacac aaccggaagg cgggttgatc ggcgacggta attccccttc cagtaactgg
    14341 atggttttgt tcttctccag atccggatca ggtatgggga ctgccgacat caatgccctg
    14401 gtgtaaggat gtagtggatt gtggtagacc tcatcatagg tccccagttc taccgcatgg
    14461 ccgagataca tcaccaacac acgatcggaa atgtgtttta ccacggccag gtcatgagcg
    14521 ataaaaatta atgacaatcc catctcacgt tgcagctgct ggagcaggtt gaccacctgc
    14581 gcctgaattg acacgtccag cgccgaaacc ggctcatcgc agataatcag cttcggttca
    14641 agaataagag cacgagcaat cccgatacgc tggcactgcc caccggagaa ctcatgcgga
    14701 tagcggttaa tcaggttagg caataacccg actttcagca tcatcgcctt cacgcgctcg
    14761 cgaacttcct ggcgtgacat tttcggatga taagtacgca gtggttcagc gatgatctcg
    14821 ccgatggtca tacgcgggtt tagcgatgcc aacggatcct ggaaaatcat ctgaatatca
    14881 ctgcgaacgg cacgccattc atcgggcttc atgcccagca actctttacc taaccaggca
    14941 acatgaccgt cggtcgcctt gaccaaaccg atgatggcgc gagcaaaggt ggacttaccg
    15001 catcccgatt cccctaccac acctaatgtt tccccttcat acaggcgaag agtgacacca
    15061 tcgacggctt tgagcgtttt cggcggttgc cagaaccact gtttgccatc tttgatttca
    15121 aagtgcactt taagatcggc gatttcaagg aggacttttc ttccttcagt tacagcattc
    15181 ataacagttc ctccaccggt ttaaagcaag cacgcagacg gccaggcgta aactcttcca
    15241 gcggcggtgc gctactacaa atttccatcg catgtggaca acgtggctgg aacgggcaac
    15301 ctttcggtaa tcgcagcaag tttggcggat tacccgggat ggtcaacatt gtttcacctt
    15361 ccgcatcaag acgcggcacc gcgttgagca aaccgataga ataaggatga acgggttgat
    15421 aaaagacatc gcgcgcattg ccatattcca tcgtgcgccc ggcgtacatt accagcactt
    15481 tgtcacagat ccccgccacc acgccaagat cgtgggtgat cataatgatg gcggtattaa
    15541 attcccgctt cagttcattt aatagcgtca tgatctgcgc ctgtacggtg acgtccagcg
    15601 cagtagtggg ttcatccgca atcagtagct taggtcgaca tagcaatgcc atcgcaatca
    15661 tgactcgctg acgcatgccg ccagaaaatt cgtgcgggta cattttcatg cgtttacgcg
    15721 cttccggcat ttttaccgca tcgagcatcc gcaccgactc ttcaaacgcc tcagctttgc
    15781 tcatgttctt atgcagcatc agcacttcca ttaactgctc accgacgcgc atatagggat
    15841 tcaacgaagt cattgggtcc tgaaaaatca ttgagatttg ttcagcgcgc agtttattga
    15901 gttcatgctc tggcaaattg aggatttcac gcccattgaa ggttgccgat ccgccaatac
    15961 gtccgttggc agccaacagg cccatcaacg caaatgcagt ttgcgattta cccgaaccag
    16021 actcacctac aatacccagc gtttctccgg cacgtaggga aaaattcaaa tcattgaccg
    16081 ccgtgacgtc gccgtccggg gtactaaagg tgacacgcaa atctttcacg ttcagcagtg
    16141 cgtcagcctg ttgttgtgcg agcggcacag ttgcagtttc aattacgctc atggctgcac
    16201 tccttaacga tctttcgggt cgagggcatc acgcaagcca tcgccgataa agttgaaaca
    16261 aaacagcgtc accacgagga atcccgctgg gaacaacaat aaccatggag agacttccat
    16321 cgagttcgcg ccatcactca gcaatgcacc ccagctgctt aacggctctt gcgtacccaa
    16381 ccccaggaag ctaaggaaag attcaaagag gatcatgctg ggcaccagaa gtgatgcgta
    16441 gaccaccacc acaccgagta cattcggcac aatgtggcga ataacaatgc ccgatgtcga
    16501 tacaccgcca acttgtgccg cctcaataaa ctctttgcgc ttcagactca gggtttgccc
    16561 acgcacaata cgagccatat ccagccagga aaccatgcct atcgccacga aaatcagcag
    16621 gatgttttga ccgaaaaagg tcaccagcaa aatgacgaag aacatgaatg ggaaggagtt
    16681 gaggatttcc agcagacgca tcattaccga atccacttta ccgcccagat aaccggaaag
    16741 cgaaccataa agtgtcccga cgaccacagc caccagtgcc gcagcaacac cgaccatgag
    16801 tgagatacgc ccgccaatcg caacgcgcac aagcaggtcg cgaccggatg agtcagtacc
    16861 aaagtagtga ccggactcca tatccggggc gctggacatc atcgcccagt cagtatcgtc
    16921 ataggcaaac tgggaaagca tcggtgccag gattacaaat aacgcgatca gcaccagcac
    16981 tatcagactg gcaaccgccg cacggttatg cataaaacgt cgacgtgcgt cctgccacaa
    17041 gctgcgccct tcgacctcca gcttttcact gaaattttcc agcgtctcgc tgtttttctt
    17101 acttaacatc atcgcgagct ccagtatcag taacggattt tcgggtcgat aaccgcatat
    17161 agcacatcga caatggcatt aaacaaaatg gttaaagcac caaccaggat ggtcaggctt
    17221 aacactaagg aatagtcacg gttcaatgcg ccattaacga acagttgtcc aatccccggc
    17281 aaaccataaa tggtttcgat aaccatagaa ccggtaataa tgccgacaaa tgcggggccc
    17341 atataagaga gcacgggtaa cagagcaggt tttaatgcgt gacgtaaaat gatccgccgc
    17401 ataggtaacc ctttcgcccg ggcagtacga ataaaattgg agtgtaatac ttcaatcata
    17461 gagccacggg taatacgcgc aatactggcg atataagcga gtgacaacgc caccatcggc
    17521 aatatcatga atttaagagc cccaccattc cagccaccgc ccggcagcca atgcaaaatg
    17581 atcgcaaata tcatgactaa taatggcgca accacaaaac tggggataac aaccccggtc
    17641 attgccagcc ccatcacggt atagtcccat ttggtgtttt gttttaatgc ggcaataacg
    17701 ccagcactaa cacccagtat taccgcaagg aaaaatgctg cggctcccag tttggcagaa
    17761 acgggaaaac tggatgcaac caggtcattg accgaataat ctttatattt aaacgatgga
    17821 ccgaaatcac cgtgcgccag ttgtttcagg taactgaaat actgtgtcat gatcggatca
    17881 ttaagatgat atttcgcttc gatattggcc atcacttccg gcggtaaagt acgttcgccg
    17941 gtaaaagggc ttcccggcgc gaggcgcatc ataaagaacg aaatagtaat aagaataaat
    18001 agcgtcggaa tcgcttccag acagcgacgt agaataaatt ttaacattgc cctaccttct
    18061 ggcctgtgcc ttctgtaatg cgataaaaat cagacaccgt ggagcaggac actcctgccc
    18121 cacgtattgc cattagtgct tcacaatgta catattccgg gtgtaggtat tatccagcgg
    18181 atctttgccg gtatagccac caacccacgg tttcaccaga cgcgcattca cgtagtaata
    18241 aacaggaaca atggccgaat ccttatccag ctgttgttct gctttagtgt acagagctgt
    18301 gcgctgcgcc tcgtcagtca ctttcagcgt ttccgccata atgctgtcaa aggccgggct
    18361 cttataatgc gcggtattca tcgagctgtt cgacagcatg gtgttcagga aggaagttgg
    18421 ttcgttgtag tctgcacacc agcctgcacg ggccacatca aaagtgccct ggtgacgggt
    18481 gtcgaggaac gttttccact cctggttaac cagtttgacg tttacaccaa tgtttttctt
    18541 ccacaatgaa gaggcagcaa tcgccagctt tttatgcaga tcggaggtgt tatacagcag
    18601 gttgatggtc aacggtttgt ctgcggtata acccgcttca gccagcagtt ttttcgcttc
    18661 ttcgttacgt ttttcctggc tccagccaaa ccattctggc tgagtcaatt ttgcgccatc
    18721 agtatacggt ggagtgtaac cataggcggg catgtcgccc tgcgctttca ctttattaac
    18781 aatgatatcg cggtccatac ctagtttcag tgcggtacgc acacgcacat cgttgaatgg
    18841 cggtttctgg ttgttaattt cgtaatagta agtgcacagg tatggatcaa cgtgaacttc
    18901 gtccgggatc tcttttttca gcttctggaa caattcgatc ggcatgttgt tataagtcat
    18961 gtcgatttca ccactacggt agcggttgac atcggtaact tcagaagcaa taggcaaata
    19021 ggttacctgg ttaataacgg ttttcgcgtt gttccagtag gtcgggctgc gttcaagaac
    19081 gattcgttcg tttacgaccc aatcttttaa ggtataggca ccgttggtga cgatattacc
    19141 aggctgggtc catttttcgc cgaatttctc gatagcggct tttggcaccg gtgaagttga
    19201 tgggtgaaca agtaatttat agaagtacgg aacgggttca cttaaggtga cttctaatgt
    19261 gtgatcatca atagctttca cgccgagatc ggtaatcggt tttttccctt caagaatttc
    19321 atcaataccg gcgatatgcc catattgcag ataactggca tacggagaag cagtgttcgg
    19381 atcaacagaa cgttgccagc tatacacaaa gtcttgtgct gtgactggcg taccatcaga
    19441 ccatttcgca tctttacgca aatggaaggt ccagactttc gcgtctttat tatcccagga
    19501 ttcagcgacg ccaggtgccg gatgaccgtc aagatcgctg accagtaagc cttcaaacag
    19561 gtctcggctg atattagact ccggaacacc ttcaattttg tgcggatcta atgactgaac
    19621 ttctgaacca ttgttacgta ccagcgtttg tttttccgcc agtgtgacgc ctgcgggtac
    19681 atcagctgcc agcgcgacat tccctgccat tagcgcagcc agaacgccag ctgctactaa
    19741 acttctcttg gtgatgttgg tcattgtttt ttggactccc tcattataat tactggtatt
    19801 aaccagcatg tgtaatcccg gattgggacc ctgcacaggc tcggagactt tctgctgtca
    19861 ggttacttta tcgttacgga ttgctatcac cgactttatt tattctggtg attcagacga
    19921 tcaccttatg tcgattcgta ctgtgtctcc cctgtcgaga cacattattc tttcgcgacg
    19981 ctctttaatt gttaattatt ctcattacca ataaagaatc gtctggcggt tgcccggaaa
    20041 gtaccaaatg cggttcttac tcgccaatac attttgcaaa attgttaagc aattctcttt
    20101 tattgaccgt atcagaatct atagcttgca tccatgcctt aacaaacatc tttcaattca
    20161 atcaaataga gattggctaa gcatgtctat cttttctgat tacagttcca gtagtgaaat
    20221 gcacaataat ttaacaattg attattattt agcactttcc tctaccaaag gcagtggaat
    20281 tactaatatc atttcaatta ttcttcagca agcccaggat tatgatgtgg cgaaaataac
    20341 atgatggtga ggagaagcag ttaaggaaat ccttataacc aactgtaatt tgagaagaat
    20401 atttttacct attccttttg cttttgataa ctgcgcggga acaaagtaaa aataatgacg
    20461 aagtaaacgg agcatggcag ctccgtttca tttgaaagga attaattaag caggccgggg
    20521 aaaatcccct taataccagt aacgataaat tcaatcccca atgccatcag caatagcccc
    20581 ataatacgcg taatcacgtt gatgccggtc tggcgtaaaa cccgtaccag ccacggtgcc
    20641 atgcggaaca atccccagca acataaagcg aacaatgcaa tagccacaaa gaaaccaaac
    20701 agatagctaa tgctgtgata acgcgtaccc cagacgatgg tagaactgat cgcccctggc
    20761 cccgccatca acggcaacgc caatggcacc acaccaatgc tttcacgtac cgcggtttct
    20821 gatttttctt gcttgttctg tttatcctcg ccaagcttgc cgctgatcat cgacatcgct
    20881 atcgtcacca ccaggatgcc cccggcgata cggaacgaat caattgatat accaaaaagt
    20941 tgcagaatcg tgtcgccgag aaaaagcgag atccacaaga taatggccac agacaggttg
    21001 gctgtaaggt tagttttgtt tcgcgctgcc gctgtctgat aactggtcat gctgataaag
    21061 acgggaataa tccctaccgg gttgaccagc gcaaataacc cgatgaaaaa tttgaagtaa
    21121 acgggaaaat caaaaaaggt ctgaatcacg gttagctccg aagcaaaagc cggataatgt
    21181 tagccataaa taaggttgaa aagacgcgct gacaatacgc cttttgacag catttttcac
    21241 ctcctaacta cttaaaattg ctatcattcg ttattgttat ctagttgtgc aaaacatgct
    21301 aatgtagcca ccaaatcata ctacaattta ttaactgtta gctataatgg cgaaaagcga
    21361 tgctgaaagg tgtcagcttt gcaaaaattt gatttggatc acgtaatcag tacccagaag
    21421 tgagtaatct tgcttacgcc acctggaagt gacgcattag agataataac tctaatgttt
    21481 aaactctttt agtaaatcac agtgagtgtg agcgcgagta agcttttgat tttcataggt
    21541 taagcaaatc atcaccgcac tgactatact ctcgtattcg agcagatgat ttactaaaaa
    21601 agtttaacat tatcaggaga gcattatggc tgttactaat gtcgctgaac ttaacgcact
    21661 cgtagagcgt gtaaaaaaag cccagcgtga atatgccagt ttcactcaag agcaagtaga
    21721 caaaatcttc cgcgccgccg ctctggctgc tgcagatgct cgaatcccac tcgcgaaaat
    21781 ggccgttgcc gaatccggca tgggtatcgt cgaagataaa gtgatcaaaa accactttgc
    21841 ttctgaatat atctacaacg cctataaaga tgaaaaaacc tgtggtgttc tgtctgaaga
    21901 cgacactttt ggtaccatca ctatcgctga accaatcggt attatttgcg gtatcgttcc
    21961 gaccactaac ccgacttcaa ctgctatctt caaatcgctg atcagtctga agacccgtaa
    22021 cgccattatc ttctccccgc acccgcgtgc aaaagatgcc accaacaaag cggctgatat
    22081 cgttctgcag gctgctatcg ctgccggtgc tccgaaagat ctgatcggct ggatcgatca
    22141 accttctgtt gaactgtcta acgcactgat gcaccaccca gacatcaacc tgatcctcgc
    22201 gactggtggt ccgggcatgg ttaaagccgc atacagctcc ggtaaaccag ctatcggtgt
    22261 aggcgcgggc aacactccag ttgttatcga tgaaactgct gatatcaaac gtgcagttgc
    22321 atctgtactg atgtccaaaa ccttcgacaa cggcgtaatc tgtgcttctg aacagtctgt
    22381 tgttgttgtt gactctgttt atgacgctgt acgtgaacgt tttgcaaccc acggcggcta
    22441 tctgttgcag ggtaaagagc tgaaagctgt tcaggatgtt atcctgaaaa acggtgcgct
    22501 gaacgcggct atcgttggtc agccagccta taaaattgct gaactggcag gcttctctgt
    22561 accagaaaac accaagattc tgatcggtga agtgaccgtt gttgatgaaa gcgaaccgtt
    22621 cgcacatgaa aaactgtccc cgactctggc aatgtaccgc gctaaagatt tcgaagacgc
    22681 ggtagaaaaa gcagagaaac tggttgctat gggcggtatc ggtcatacct cttgcctgta
    22741 cactgaccag gataaccaac cggctcgcgt ttcttacttc ggtcaaaaaa tgaaaacggc
    22801 tcgtatcctg attaacaccc cagcgtctca gggtggtatc ggtgacctgt ataacttcaa
    22861 actcgcacct tccctgactc tgggttgtgg ttcttggggt ggtaactcca tctctgaaaa
    22921 cgttggtccg aaacacctga tcaacaagaa aaccgttgct aagcgagctg aaaacatgtt
    22981 gtggcacaaa cttccgaaat ctatctactt ccgccgtggc tccctgccaa tcgcgctgga
    23041 tgaagtgatt actgatggcc acaaacgtgc gctcatcgtg actgaccgct tcctgttcaa
    23101 caatggttat gctgatcaga tcacttccgt actgaaagca gcaggcgttg aaactgaagt
    23161 cttcttcgaa gtagaagcgg acccgaccct gagcatcgtt cgtaaaggtg cagaactggc
    23221 aaactccttc aaaccagacg tgattatcgc gctgggtggt ggttccccga tggacgccgc
    23281 gaagatcatg tgggttatgt acgaacatcc ggaaactcac ttcgaagagc tggcgctgcg
    23341 ctttatggat atccgtaaac gtatctacaa gttcccgaaa atgggcgtga aagcgaaaat
    23401 gatcgctgtc accaccactt ctggtacagg ttctgaagtc actccgtttg cggttgtaac
    23461 tgacgacgct actggtcaga aatatccgct ggcagactat gcgctgactc cggatatggc
    23521 gattgtcgac gccaacctgg ttatggacat gccgaagtcc ctgtgtgctt tcggtggtct
    23581 ggacgcagta actcacgcca tggaagctta tgtttctgta ctggcatctg agttctctga
    23641 tggtcaggct ctgcaggcac tgaaactgct gaaagaatat ctgccagcgt cctaccacga
    23701 agggtctaaa aatccggtag cgcgtgaacg tgttcacagt gcagcgacta tcgcgggtat
    23761 cgcgtttgcg aacgccttcc tgggtgtatg tcactcaatg gcgcacaaac tgggttccca
    23821 gttccatatt ccgcacggtc tggcaaacgc cctgctgatt tgtaacgtta ttcgctacaa
    23881 tgcgaacgac aacccgacca agcagactgc attcagccag tatgaccgtc cgcaggctcg
    23941 ccgtcgttat gctgaaattg ccgaccacct gggtctgagc gcaccgggcg accgtactgc
    24001 tgctaagatc gagaaactgc tggcatggct ggaaacgctg aaagctgaac tgggtattcc
    24061 gaaatctatc cgtgaagctg gcgttcagga agcagacttc ctggcgaacg tggataaact
    24121 gtctgaagat gcgttcgatg accagtgcac cggcgctaac ccgcgttacc cgctgatctc
    24181 cgagctgaaa cagatcctgc tggataccta ctacggtcgt gattatgtag aaggtgaaac
    24241 tgcagcgaaa aaagaagccg ctccggctaa agctgagaaa aaagcgaaaa aatccgctta
    24301 atcagtagcg ctgtctggca atataaacgg ccccttctgg ggtaatgccg atcagttaag
    24361 gatcagttga ccgatccagt ggctgtgtaa gaatccggaa acgctcactc gtttccggat
    24421 ttttttatgc acattggaca ggcccttgat ttggtatccc gttacgattc tctgcgtaac
    24481 ccactgactt ctctggggga ttacctcgac cccgaactca tctctcgttg ccttgccgaa
    24541 tcaggtactg taacgctacg caagcgccgt cttcccctcg aaatgatggt ctggtgtatt
    24601 gttggcatgg cgcttgagcg taaagaacct cttcaccaga ttgtgaatcg cctggacatc
    24661 atgctgccgg gcaatcgccc cttcgttgcc cccagtgccg ttattcaggc ccgccagcgc
    24721 ctgggaagtg aggctgtccg ccgcgtgttc acgaaaacag cgcagctctg gcataacgcc
    24781 acgccgcatc cgcaatggtg cggcctgacc ctgctggcca tcgatggtgt gttctggcgc
    24841 acaccggata caccagagaa cgatgcagcc ttcccccgcc agacacatgc cgggaacccg
    24901 gcgctctacc cgcaggtcaa aatggtctgc cagatggaac tgaccagcca tctgctgacg
    24961 gctgcagcct tcggcacgat gaagaacagc gaaaatgagc ttgctgagca acttatagaa
    25021 caaaccggcg ataacactct gacgttaatg gacaaaggtt attactcact gggactgtta
    25081 aatgcctgga gcctggcggg agaacaccgc cactggatga tccctctcag aaagggagcg
    25141 caatatgaag agatcagaaa actgggtaaa ggcgatcatc tggtgaagct gaaaaccagc
    25201 ccgcaggcac gaaaaaagtg gccgggacgg ggaaatgagg tgacagcccg cctgctgacc
    25261 gtgacgcgca aaggaaaagt ctgccatctg ctgacgtcga tgacggacgc catgcgcttc
    25321 cccggaggag aaatggcgga tctgtacagt catcgctggg aaatcgaact gggatacagg
    25381 gagataaaac agacgatgca actgagcagg ctgacgctga gaagtaaaaa gccggagctt
    25441 gtggagcaag agctgtgggg tgtcttactg gcttataatc tggtgagata tcagatgatt
    25501 aaaatggcgg aacatctgaa aggttactgg ccgaatcaac tgagtttctc agaatcatgc
    25561 ggaatggtga tgagaatgct gatgacattg cagggcgctt caccgggacg tataccggag
    25621 ctgatgcacg atcttgcaag tatgggacaa cttgtgaaat taccgacaag aagggaaagg
    25681 gccttcccga gagtggtaaa ggagaggccc tggaaatacc ccacagcccc gaaaaagagc
    25741 cagtcagttg cttaactgac tggcattacc ccttctgggg ccgttttttt gtttacccaa
    25801 agcaactttt ccataaaccg acagcattag ccttcatcat atttgcgacg atgtataacg
    25861 cctaaacaca gggatattgt actttacagg tcacaagtca acgtcggtgc ttaagagccc
    25921 tgtgaggcgt atagcggcgt taaaaaactg ccgagaaggg tatatagccc ggaagaagtg
    25981 cgtaaaacga actgacagga taaaagtgcc ctgctcaccc tgtcagtaaa gaaattctta
    26041 ttaatcgtgg cgatgccttt cctgaatagc cgttaatgag ccgacttgta acgcctcttt
    26101 atagtgttta cggcatacag aaacgtatcg ttcattacca ccaattacca cctgctcacc
    26161 ttcgttataa ggtctgcctg cttgatcaag acgcagcacc atgcttgctt tacggccaca
    26221 aaaacagatg gtttttaatt caaccagttt gtcggaccat gccagtaagt attggctgcc
    26281 aataaataat tcacctcgaa aatcggtacg taaaccataa caaagtacgg gtatatcgag
    26341 ttgatcgaca acctccgata attcatatac ttgttgtctg gttaaaaact ggcattcatc
    26401 aaccagtacg caatgaattg cctgctgttc atgttccgca cgaatctcat caaataatga
    26461 tgaattttgg ttaaataatt ttgcaggcga tgacaaacct atacgcgaac tgactttccc
    26521 ggcaccaaag cgatcatcaa tttctgccgt atatacgaca gtgcgcatgc cgcgttcctg
    26581 gtaattgtat gaagattgca acaatgctgt agacttaccc gcattcattg cggaatagta
    26641 gaaatatagc tgtgccatca gccacaggcc ctcaatgata tgaaataaag ttggctggag
    26701 tttatcataa ttcgtcagtt tttcagctca acagtatgca cagaagtggg tatatgcgtt
    26761 ctcccttacg aagccttgca taatccttct gagctatcat tacaactgcc tcgcttgtta
    26821 taagcgggta aatgactgct ggtaactatt cacaatcttt aacctgttgc gcaagtaata
    26881 gccctctgtt gacctccagg agatagtgca atactaagtc catgctctta ttgcgactgt
    26941 tctacttttc atcattcgct taatagggaa ttctcgtaaa cacaactaat acagaagact
    27001 gaaaggtcgt cagcctacga taatctcccc ataaaatgtg acatgaatca ggaagtttta
    27061 acctcacgtg ctgcgaaatc atcggtgtaa atagggctat atgccgcgtc ttttctggct
    27121 aattttatga aaagatattt attggcggca caaaataaag aacaattttg aattccttac
    27181 attcctggct attgcacaac tgaatttaag gctctattat tacctcaaca aaccacccca
    27241 atataagttt gagattacta caatgagcga agcacttaaa attctgaaca acatccgtac
    27301 tcttcgtgcg caggcaagag aatgtacact tgaaacgctg gaagaaatgc tggaaaaatt
    27361 agaagttgtt gttaacgaac gtcgcgaaga agaaagcgcg gctgctgctg aagttgaaga
    27421 gcgcactcgt aagctgcagc aatatcgcga aatgctgatc gctgacggta ttgacccgaa
    27481 cgaactgctg aatagccttg ctgccgttaa atctggcacc aaagctaaac gtgctcagcg
    27541 tccggcaaaa tatagctacg ttgacgaaaa cggcgaaact aaaacctgga ctggccaagg
    27601 ccgtactcca gctgtaatca aaaaagcaat ggatgagcaa ggtaaatccc tcgacgattt
    27661 cctgatcaag caataatctt ttgtagattg cacttgctta aaatcccgcc agcggcggga
    27721 ttttttattg tccggtttaa gacaatttaa taagaataag ctataaaaaa acggcgtcga
    27781 ttgctcaacg ccgtttcgtg gataacaccg atacggatgt tacttcttaa tgcccatctc
    27841 ttcttcaagc caggctttaa attccgtgcc aagggtgtta tgacgaatac cgtattcaac
    27901 gaaggcctgc atgtaaccta atttattacc gcagtcatgg ctcttccctt tcatatgata
    27961 ggcttccacc gtttcttttt cgatcagcat atcaattgcg tcggtgagct gaatttcatc
    28021 accagctccc ggaggggttt ttgccagcaa cggccaaata tccgcgctaa gtacgtaacg
    28081 acccacaata gcgagattag acggcgcaac atccgctttc ggtttttcta ccacaccaac
    28141 catcggtacg ctttcaccag gcgctaattc aacgcctttg caatccacaa cgccatatgc
    28201 ggtcacatca gcaaccggtt caaccatgat ctggctatga cccgtttcat caaagcggcg
    28261 gatcatctct gccaggttat cctgtgacaa atcggattca tattcatcca gaataacatc
    28321 aggcaaaata acagctaccg gttcatcacc cactaccggg tgagcacaca ataccgcgtg
    28381 tcccaggcct ttcgccagac cctgacgaac ttgcataata gtcacgtgcg gtggacaaat
    28441 agactgcact tcatcaagca gttgacgttt tacacgtttt tccagcattg cttccagttc
    28501 aaaactggta tcaaagtggt tttcaataga gtttttagat gagtgtgtaa ccagcacaat
    28561 ttcagtaatg ccagccgcaa tacattcatt cacgacgtat tgaattaatg gcttatcgac
    28621 aagtggcagc atctctttcg ggatggcttt cgtcgccggc aacatcctgg ttcctaatcc
    28681 cgcaacgggg ataacggctt ttttgacttt cgtattaatg gcagccattt aaattctcct
    28741 ggactgttcg tgttttgaac gtgttcatat ttctgtatcg catcccagta tatcagcacc
    28801 ccgaaggggc taagttctga aaaggaggcg agttagcata attaagacaa ttacttatac
    28861 gaatggcgac gatagtacca ccagccgaca ttagcaggta atgcaaattt agcccgcgtt
    28921 atcgtttgct cattctgcag acaacatcaa gcgcagtcga ccaccggttc cccatatttg
    28981 gcattgccag gcatcgcatc gctggctcaa ttgattcaaa taagcgttac ctaaagtgcc
    29041 taacggaaca ccattactga tttgcacctg gtgttcgccg gtatttaacg tcgcattcag
    29101 acccgcagaa accagaatga gatttttcag ttcgcgatga taatagccaa ctaatagcgg
    29161 aaactgcccc ggcagattgg cctgacgaag taaatggttt acctgcttca ataacgcgcc
    29221 caactctggc aaccgttgat tttggtgtgc aagctgttcc tgtaataatc cgttaaacaa
    29281 tgcgcgtaat aacaaggcag caagtacgcc attatgtcca gctcgggtga catcaaggca
    29341 ataaaatgcc agatcgtttt ccgaaagtgc ggcaatatca agcaccaggc cgggtttgtc
    29401 cgcggcaacc aattgacgat aattaacccg gcaatgggaa atcacctgct gaaccggcgg
    29461 ttgtagttcc tgtaataatt tcgccgctgc ggcagggtta tcaaccattg catcccagtc
    29521 gcgaaaaagc ctttcctctt cctcaacgcg cgaattaaac atgctgggat agagacaggc
    29581 aaaaaccatc tcgcgcaagc gattcagatc tttaactggt ttcagcaaaa catcttcaac
    29641 gcccagacgt aacgctttgg caatatctgc catattttca gtggcagata tcaccagaac
    29701 cggggtctgg tcgcctctgt tacgtatatg ctccagcagt ttaagcccgt tcattcgtgg
    29761 catcgcgata tcacatatca tcaggtctgg agtgaaacct cccagcaact caagggcatc
    29821 caccccatca gccgccagta ccgttgtcgc tcccaatgag gaaaaccatg aatccagaag
    29881 cgagcgaaat acctgctcat cttcaacaat gagaatctgt tttccgacca atggctgcgt
    29941 catgttctct cccctgactg gctttactca atagtggcat gctattgcca cctgcgcctg
    30001 tcagaattaa cttaagtgta gataaaaatt ctggtcaaat gttggtgcgt accaacggca
    30061 aaagttcgtc cattttcctt tccactgcca gctgtccggc cgcaatggca gcgtgcgcgc
    30121 gatggaaatc aagcgtagat atttgcgggc aaacaggttg aatcagaata tcgggcggat
    30181 cacctgccat gcggttcctt ttaaggcggt tctccagcac ctggatagaa gtggtcataa
    30241 tctctgttgc cgttggtgct gtcaccgccc gacgtgtcgt tatgctgcct aacctttctt
    30301 tcagacgcgc atgccacggc agagaatcac cattctcgct attttcttca ctgacattaa
    30361 aggagagcaa atcttgttgc attaaatgag catcgtgctg caggtcgacc gctatcacaa
    30421 tatcagcacc caatgcacgc gtgagcgaaa taggaattgg gttaacgact gccccatcaa
    30481 ccagccagta gccgttatgc gcgactggtg ccatgagtcc tggaatactg catgacgcgc
    30541 gaatagcaag atggagatcg ccttcagtaa accataattc acgcccagta cttaaattgg
    30601 tggcaacagc cgcgaagcga cgggaacaat tttcgatctc tgtttccggc attatctcgc
    30661 ggtattgatt gaagacacgc tcgccgcgca gtaacccacc gcgctgccag gagagatcca
    30721 tcaggcgtaa aacatcccaa taactgaaag aggttaccca atcttccagc gcagataatc
    30781 gatcgcatgc ataggccgcg cccaccagcg aaccaattga acatcctgca acgatatcaa
    30841 tttcaatacc cacttttttt agcgcattaa taacgccaat atgcgaccaa cctctcgccg
    30901 cgccagatcc cagcgccagc cctattttta tctttctcat tattcccgca agattcccct
    30961 ggaatgcaat cgtagccaca tcgctactgc tcggttaaca tagtgctact caggcggtta
    31021 atacgccgtt attgtttcca gggagattat ttgtgtctca gctttgtccc tgtggtagtg
    31081 ctgtcgagta tagcctatgt tgccaccctt atgtgtctgg tgaaaaggtt gcacctgatc
    31141 cagaacatct catgcgttcg cgttactgcg cttttgtgat gcaagacgca gattatttaa
    31201 taaagacctg gcatccatct tgtggagcag cggcgttacg tgccgaattg attgccggat
    31261 ttgcgcatac tgaatggttg ggattgaccg tatttgaaaa ttgttggcag gacgccgaca
    31321 atatcggatt tgtcagtttt gttgctcgat ttaccgaagg tggtaaaaca ggtgcaataa
    31381 ttgaacgttc gcgatttttg aaagaaaacg gtcaatggta ctatattgac ggtacacgtc
    31441 cgcagtttgg tcgcaacgat ccctgccctt gtggttcagg taaaaaattt aaaaagtgct
    31501 gcggccaata atggttgacg gtacggttta gcaaacactc tcaacaaggt ttttccagca
    31561 atgcattcac tccaacgtaa agttctgcgt actatttgtc cggaccaaaa aggtctgatc
    31621 gcacgcatta ccaatatttg ctacaagcac gagttaaata tcgtacagaa caatgaattt
    31681 gttgatcacc gtaccgggcg cttttttatg cgcacggaac tggaagggat ttttaatgat
    31741 tccacccttc tggcggatct cgatagcgca ttgccagaag gctccgtgcg tgagctgaat
    31801 cctgccggtc gtcgccggat agtgattctg gtcactaaag aagcgcattg ccttggcgat
    31861 ttgttgatga aagccaatta cggcggcctg gatgtcgaaa tcgcggcagt tattggtaac
    31921 cacgatactt tacgttctct ggttgagcgt tttgatattc cgtttgagct ggtaagccat
    31981 gaagggttaa gccgcaacga gcacgatcaa aagatggcgg acgccattga tgcttatcaa
    32041 cctgactatg tggtgctggc gaagtatatg cgggtattaa cgccggaatt tgtggcacgc
    32101 ttcccgaata aaatcatcaa tattcaccat tcattcctgc ctgcgtttat cggcgcacgt
    32161 ccttatcacc aggcctatga acgtggtgtg aagattattg gcgcaaccgc tcactatgtg
    32221 aatgacaatc tggacgaagg cccaatcatc atgcaggacg ttattcatgt cgatcatacc
    32281 tacacagctg aagatatgat gcgcgcaggt cgtgacgtcg agaaaaacgt cttaagtcgt
    32341 gcgctataca aagtactggc acagcgcgtc tttgtttacg gtaatcggac gattattctt
    32401 taatcgccag caaaaataac tggttacctt taatccgtta cggatgaaaa ttacgcaacc
    32461 agttcatttt tctcaacgta acactttaca gcggcgcgtc atttgatatg atgcgccccg
    32521 cttcccgata agggagcagg ccagtaaaaa gcattacccc gtggtggggt tcccgagcgg
    32581 ccaaagggag cagactgtaa atctgccgtc atcgacttcg aaggttcgaa tccttcctcc
    32641 accaccatct caagtattac ctcaaaattt gaattacccc tggtggggtt cccgagcggc
    32701 caaagggagc agactgtaaa tctgccgtca tcgacttcga aggttcgaat ccttccccca
    32761 ccaccatcac ttttcaaaag tccgtaaact ctcaaacgaa tccgcaatta aatattctgc
    32821 ccaagcgggg aaggatgaga agcttcgacc aaggttcgac ttgagcgcca gcgagagagc
    32881 gttgccgcag gcaacgaccc gaagggcgaa gcgcgcagcg ctgagtaatc cttcccccac
    32941 caccatcgct tccttaaata atcaaaacct gttactccga gctaattttg cccgatggca
    33001 ccacgtttac cgggcctaca atatcctccc ctgtaggtcg gataagcgaa gcgcatccga
    33061 caactcaccc cgcaccgtgc ccataaaaaa accccgccga agcagggtct atgtgaacta
    33121 aaattcgctt agtgacgagc gcgcaccagc tggtacttac gtgtcagata ctccaccggt
    33181 acgctccaga tgtgtaccag acgcgagaac gggaacagca ggaataacgt catccccagc
    33241 accaggtgca gacggaagat aaacgccaca ccatcgaggt gttgagaagc gccaccgtgg
    33301 aaggtcacca ccgactgcgc ccagccaacc agtttcatca tctcgctacc gtccatatgc
    33361 tgagcggaga acggaatggt caacaggccc agcgcgcact ggataacgag cagcgacagg
    33421 atcaggatat ccgctccggt agtggttgca cgcacgcgtg ggctgaacag acgacgcttc
    33481 agcaacagca cgccgccaat cagacacagc acgccgctgg caccaccagc aaacatcgcc
    33541 attttctgtt tcacttcaat cggcagccag gcttcataca tccagtgcgg cgtcagcata
    33601 ccgaagaagt gaccaacaaa aatccccaga ataccgatat ggaacaggtt cgacgccagg
    33661 ttcatccctt tgcgatccag catctggctg gacgccgcgc gccaggtgta ctgcccgtag
    33721 tcataacgca gccagctacc aatcaggaag accgccccgg ctatgtacgg gtagatatca
    33781 aagaagaaca tattcaggaa ttgcattagt gctgtcctcc ggtggtgata ttcagatatt
    33841 gcggcgcgac ggcaccggca aagcgacgct gatgagcagt gattgctgaa tcaccgcagc
    33901 ctttgtcagc aaagaattta acctgctctt cttcccagac agcatccagc gcctgcggcg
    33961 tatcatcgcg cgcttcgtcg gcaatttttt ccgccacttt gtcgctgtcg atagcggtat
    34021 tggccaattt cagcagcaga tcaaacatca cggcataacg gctttcacgc tgttgcagac
    34081 gcgcgctcag caatgccaga atcggcgcga tatctttcaa accttccacg gcttcgcttt
    34141 gcggcagctg cgacaggtac tccagataca gcggcagatg gtccggtaat tcgcggctgt
    34201 ttaactgcaa gccgtgctgc tcgtactgcg ccagcaggtc caccatcgcc tggccgcggt
    34261 cacgggattc accgtgaaca tgttcaaata gcaacagtga cgtggcgcgg ccacggtcga
    34321 acagttcgct gtactgggcc tgggcatcga gcggatccat cgccgttaaa tcgcgcagga
    34381 aaatgcccag cgcatgggca tcttctttcg acagattttt cgacgcagca atcgcctcaa
    34441 acatctcttg ttgatgctgc cataaggcag catccggata ttcaaggaga cgcgatacaa
    34501 tcacgagttc gatcatggat gcggctccgt tttgctggtc acatcgatgg catcgatacg
    34561 acggctgttg aacagattga atttggtatc tgaaccgtgg cagccatcac caaaggtaaa
    34621 gccgcagcca tttttctccg ggaaggcttc ccgtgccagt tcacgatgac tactcggcac
    34681 cacaaagcga tcttcgtagt tagcaatcgc cagataacgg tacatctcct gtgcctgggc
    34741 ttcggtcaga ccgacctctt ccagcgcacg ggtatcaact ttaccgtcaa cggtttcagc
    34801 acgtttgtaa tgacgcatcg ccagcatacg tttcagtgcg cgcagtaccg gtttggtatc
    34861 accggcggtc agcagattcg ccagatactg taccggaata cgcaagcttt cgacgtctgg
    34921 cagaatgccg ttgctaccca gctcacccgc gtctgctgca gactgaatcg gagacagagg
    34981 cggcacgtac cagaccatcg gcagtgtgcg atattctgga tgcagcggca gcgccagttt
    35041 ccattccatt gccattttat aaaccggcga ctgctgtgcg gcttcaataa cgctcagcgg
    35101 aataccgtct ttaatcgcct gctcgatgac tttcggatcg ttcggatcga ggaacacctc
    35161 cagctgacgc tggtaaagat ctttctcgtt ctcggtgctg gctgcacgtt caatcgcgtc
    35221 ggcatcgtac agcagcacgc caagataacg gatacgaccg acacaggttt ctgagcacac
    35281 ggtcggctga cccgcttcaa tacgcggata gcagaagatg cacttctcag acttaccgct
    35341 cttccagttg aagtagattt ttttgtacgg gcatccggtg atgcacatac gccagccacg
    35401 gcatttatcc tggtcgatca ggacgatgcc atcttcttca cgcttgtaaa tcgcaccgct
    35461 cgggcaggtc gcaacacatg ccgggttcag gcagtgttcg cacaggcgcg gcaaatacat
    35521 catgaaggtg ttttcgaact ggctatacat cgccttctgg atgttgtcga agttcttgtc
    35581 tttcgccagt ttgtcaaact caccgcccag gtcatcttcc cagttcggcc ctttttcgat
    35641 tttcgccatc cgttccccgg taatcagcga acgcggacgg gcaatcggct gcgatttgct
    35701 gccttccggc gcggtatgca ggttctgata gtcaaaatcg aacggctcgt aataatcgtc
    35761 gatccccggc agatgcgggt tagcgaagat tttacccagc aacatggcac ggttacccat
    35821 gcgcggctgc agtttgccgt tgattttacg gatccagccg cctttgtatt tttcctggtt
    35881 ttcccagtca gtcgggaagc cctgacccgg cttggtttcc acgttgttga accacgcgta
    35941 ttccacgcct tcacggctgg tccagacgtt tttacaggta actgaacagg tgtggcagcc
    36001 gatgcactta tcgagattca gcaccatgcc gacttgtgaa cgaattttca ttttacgctc
    36061 tcctgtacct ggtcattgcc ttcgccatct aaccagtcaa tgttcttcat cttacgcact
    36121 acaacaaact catcgcggtt agaacctacg gtgccgtagt agttaaagcc gtatgccaga
    36181 tgggcatagc cgccgatcat atgcgtcggt ttcggcgtga tacgggtgac cgagttatgg
    36241 ataccaccac gctgttgggt aatttccgaa ccaggcaggt taacgatacg ttcctgcgcg
    36301 tggtacatca tggtcatccc tgccggaaca cgctggctga caaccgcacg ggcagtcaga
    36361 gcaccgttgc tgttgaagac ttcaatccag tcgttatcgg cgatacccag atctttggca
    36421 tcggcttcac tcaaccagac caccggacca ccgcgaccta aagtcagcat cagcaggttg
    36481 tcgctgtagg tggagtggat accccacttc tggtgcggcg tcaggaagtt gagcgctttt
    36541 tccgggttgc cgttggattt ctggcccatc acttctttca ccgaacgggt gtcgatcggc
    36601 ggacgataaa ccagcaggct ttcaccgaaa tcacgcatcc attggtgatc ctgatacagc
    36661 tgctgacggc cagagagcgt acgccacggg atcagctcgt gaacgttggt gtaaccggcg
    36721 ttgtaagaaa cgtgttcatc ttcaaggcca gaccaggtcg ggctggagat gattttgcgc
    36781 ggctgtgcct gaatatcgcg gaagcgaatc ttctcgtctt ctttattcag cgccagatgc
    36841 gtatggtcac gaccggtaaa ctcgctcagg gcagcccagg ctttcacggc tacctgaccg
    36901 ttggtttccg gtgccagcgt caggatcatc tctgccgcat caattgcggt gttcagcatc
    36961 ggctggcctt tcgccggacc ttccgctttg gtgtagttga gcttacgcag cagatccatc
    37021 tcgctctggg tgttccaggc aatcccttta ccgccattac cgattttctc catcagcggg
    37081 ccgatagagg taaagcgttc gtaagtcgcc ggataatcgc gctctacgac cataatgtgt
    37141 ggcgcggttt taccagggat caggtcgcac tcgccttttt tccagtcttt cacatccagc
    37201 ggctgcgcca gttcagcggc agagtcgtgc tggataggca gcgtgacgat gtcggtttct
    37261 ttacccagat gaccgacgca cacttcggag aatttcttcg cgatggcttt gtaaatttcc
    37321 cagtcgcttt tcgcttccca ggccggatcg accgccgcag acagcgggtg aataaacgga
    37381 tgcatatccg aagtattcat gtcgtctttt tcgtaccagg tcgccgtcgg caaaatgatg
    37441 tcggaataga gacaggtgct cgacagacgg aagtccagcg taaccaccag atccagcttg
    37501 ccttccagac cattgtcctg ccagtccact tcttccggct tcacgccgcc ctgttgcccc
    37561 agatctttcc cctggatacc gtgctccgtc cccagcaggt acttgagcat aaactcatga
    37621 cctttaccgg aagaaccgag caggttagaa cgccagatga acaggttgcg cgggtggttt
    37681 ttaccgtttt ctggttgttc tgccgcaaaa cggatggaac cctctttcag ggattccacc
    37741 gtatagtcca ccggattcat cccggctttt tccgcttcgc cagcgatagt cagcgggtta
    37801 gtgcctaact gcggtgcaga cggcagccag cccatgcgtt ccgcacggac gttaaagtcg
    37861 atcaagtgtc cggtatagcg ggatttgtcc gccatcggtg acagcaactc ttccgccgtg
    37921 acggtttcat aacgccactg gctggagtgg ttatagaaat aagaagtgct gttcatgtga
    37981 cgcgccggac gctgccagtc aagggcaaac gccagcggct gccagccggt ttgcggacgc
    38041 agtttttcct gacctacata gtgcgcccag ccgcccccgc tctgaccgac acagccgcag
    38101 aaaatcagca tgttgatcag accacgatag ttcatatcga ggtgatacca gtggttcagc
    38161 cccgcaccga cgataatcat cgaacgaccg tgcgttttat cagcgttatc ggcaaattca
    38221 cgggcgatgc gaataatctg gctgcgagaa acgccggtaa tctgctcggc ccaggccggg
    38281 gtataagctt tcacatcgtc atagctggtt gcacagttaa cgtcgttcag gccacgttcc
    38341 agaccgtagt ttgccagcgt cagatcataa acggtggtca ccagggcggt gctgccatca
    38401 gccagttgca ggcgtttcac cggcagtttg tgcagcagca cgttttccag ttccactttg
    38461 ttgaagtgtt ccgtgccgtc gccaccaaag tacgggaaac ctacctcagc gatctcatcc
    38521 tggctaccca gcaggctcag ttgcagctcg gtttcttcgc cagttttgcc gtcgcgctgc
    38581 tcaagattcc atttgccctt ctcgccccag cggaagccaa tagaaccgtt cggcgcaacc
    38641 atttcgccat tggtattaaa ggcgacagtt ttccattccg gattgttttc ctggcccagc
    38701 gcatcaacca gatcagcagc gcgcagcata cgacctgcag cgtagtaacc gtcgcgttct
    38761 tccagcatca ccagcatcgg catgtcggtg tagcgacgca catagtcggt gaaatactgg
    38821 cttgggttgt cgaggtggaa ttcacgcagc attacgtggc ccatcgccag cgccattgcc
    38881 gcatcggtgc cctgtttcgg tgccagccac agatcgcaca gtttggcgat ttcagcgtag
    38941 tctggtgtga cggcaacagt tttggtccct ttgtaacgca cttcagtaaa gaagtgagca
    39001 tccggggtac gcgtctgcgg cacgtttgac ccccaggcga tgatgtagct ggagttgtac
    39061 cagtcagcag attccggtac gtcagtttgc tcgccccagg tttgcggaga cgcaggaggc
    39121 aagtcgcagt accagtcgta gaagcttaag caagtaccgc caatcagcga gagatagcgt
    39181 gcacccgatg cgtaagaaac catcgacatt gccggaattg gcgagaagcc agcgacacgg
    39241 tccgggccgt agtttttgat ggtgtaaacg ttagatgcgg cgatcagttc gttcacttcc
    39301 tgccaggaag aacgaacaaa accaccgcgt ccacgcgcct gcttaaagct tttcgcttta
    39361 tcggcgtctt caatgataga agcccatgcc tcaaccggat cgctatgcag cgccttcgct
    39421 tcacgccaca ttttcatcag gcgtttgcgc atcatcgggt atttcaggcg gttggcgctg
    39481 taaagatacc aggagtagct ggcaccgcgc gggcagccgc gaggttcatg gtttggcaga
    39541 tccggacggg tacgcggata gtcagtctgc tgggtttccc aggtgaccag accgtttttg
    39601 acgtagattt tccagctgca ggagccggtg cagtttaccc cgtgggtaga gcggacgatt
    39661 ttgtcatgct gccaacgctg gcgatatcca tcctcccagt cacggttggt attgagaagc
    39721 tggccatgcc catcggcaaa ggtttcaccc ttctgtttga agtagcgaaa ccggtccagg
    39781 aatttactca tcggttttct cctgtgggag cctgtcggcc ctctgataaa tcgacattgc
    39841 taatgtttaa gggcaaggta acgctctgaa acagcgtggg aattgataac gatcaagtgt
    39901 gatggggatg aaaaataaag taaattccta agatacccct ttaggataat taacagttaa
    39961 agcataacat gctgtaaatt aagacgtttt atacctgtag ttatttaaag catgaagatt
    40021 tttatgcaga tgggtattaa ggagtattcc ccatgggtaa catattaatg gctcttaatg
    40081 aaatccggcg aaataagcca tataacatta gtatgttgta actaaataac gcataaaaaa
    40141 agcgcgacat catgccgcgc aaaggataat caaatattaa gatttatttt ttagaatgcc
    40201 gaccatatac cgtccaggta atcactacgc aggcgatata gaaaatcaaa aataccttca
    40261 ttgcgccgac tggcgaaccc gttaatgcca gcgagctacc aaacgctttc gggataaaga
    40321 agccaccaat cgcgccaatc gcagagatga aacccaacgc cgccgccgtg tcggttgccg
    40381 cttcacgcat cgcacgttcg tcagaacccc cttctgcttt cacgcgatcc attgtcagtt
    40441 tacggaagat cactgaaatc atctggaaag tggaaccact acccagccca gctgtcagga
    40501 acagcgccag gaagaccgcg aagaacgcca tgaagcttcc gccctgcccg tcagtcggta
    40561 aggtcaggaa cagcaggccg ctgaaaatcg ccatcagaat aaagttcacc agcgtgacac
    40621 gagttccgcc cagacggtca gataatgcac cacctgcaga acgcgccagc gcaccaataa
    40681 acggcccgaa gaaagcgtat tgcagaatct gaacatccgg gaactgcgtt tttgacagca
    40741 tcgcaaagcc cgcggagaag ccgataaagg agccgaaggt tgccagatac agcaggctca
    40801 taatccacag atgaccccgt ttgagtaccg gcaactgctc cttgatggag gctttcgagg
    40861 tagcaagatc gttcatgcca aaccacgccg caatggtgaa gatggcaagg aacggcaccc
    40921 atatccagga cgcattcgcc agatacagct cagtcccatc cggctgtttg acgccctggc
    40981 taccaaatac tgcgaaaatc gacagtgata ccaccagcgg agcaaccaac tgcataacgc
    41041 taacgcccat gttgcccaga ccaccattca ggcccagcgc gccaccctgc ttctgtttcg
    41101 gaaagaagaa gctgatgttt gccatactgg aagcgaagtt cgcgccagca aagccgcaca
    41161 gcagagagat gatgatgaag acgctataag gcgtggaggt atcctgcacg gcaaaaccca
    41221 gccagacaca aggaataatc agaataccgg tgctgaacgc cgtccagcga cgaccaccga
    41281 agattggaac cataaaggag tatggaacac gtaataacgc gccagaaact gaaggtagcg
    41341 cagtcagcat aaatagctga tctgtcgtaa aattaaagcc gactttcggt aagttcaccg
    41401 caacagcgct gaacaacatc catacgcaaa acgccagcag cagacaggga acggaaatcc
    41461 acaggttgcg gctggcaata cgttgaccgc gttgttgcca gaacgcagga tcttccggtc
    41521 gccaatctgt aatgacagct ccagtagccc tttcgggggc ggatgagtga ctcatagaca
    41581 cctctgatac tcgtttcgat gtctgccacc ttagtgtctg tagctaaagg caatttgatg
    41641 taaatcaaac gataagataa ctttatctat cattgatatt tatcattacc catagtgagt
    41701 acagtgattt cataaaaatt atgagatttt tcacagtgct gtaaaatccc tacccttacc
    41761 gatgtaaagc gactaaccac acggcaaata aggagtaact ctttccgggt atgggtatac
    41821 ttcagccaat agccgagaat actgccattc cagaatgtat cgtcacattc attaaggtta
    41881 ttgctcattt aaagcctgaa ggaagaggtt tacatgctta aacgttgtct ctctccgctc
    41941 accctggtta atcaggttgc gcttattgtg ttgctttcta ctgctattgg actggcaggg
    42001 atggcggttt ctggctggct ggtgcaaggc gttcagggca gcgcccatgc gatcaacaaa
    42061 gcgggatcgc tgcgcatgca aagttaccgt ctgttggcag cagtgccatt aagcgagaaa
    42121 gacaagccct taattaaaga gatggaacaa acggcattta gcgccgagtt gactcgagca
    42181 gcagaacgag acggacaact ggcgcaatta cagggtttac aagattactg gcgtaatgaa
    42241 ctgatccctg cgttgatgcg tgcacaaaac cgagaaacgg tgtcagcgga tgtcagccag
    42301 tttgttgccg ggcttgatca actggtatct ggttttgacc gcaccacgga aatgcgcatc
    42361 gagacagtgg tactggtcca tcgggtaatg gcggtattta tggcactttt actggtgttc
    42421 actattatct ggttgcgggc gcgactgcta caaccgtggc ggcaactgct ggcaatggcg
    42481 agtgccgtca gtcatcgcga ttttacccaa cgcgcaaaca tcagcgggcg caacgaaatg
    42541 gcgatgcttg gaactgcgtt gaacaatatg tctgcagaac tggccgaaag ttatgccgta
    42601 cttgagcagc gggttcagga gaaaaccgcc gggctggagc ataaaaatca gatcctctct
    42661 tttttatggc aggctaaccg ccgtttgcat tcccaagccc cgctgtgtga acgcctgtca
    42721 cctgtactca acggcttaca gaatttaacc ctgctacgtg atatcgaact gcgggtttat
    42781 gatactgatg atgaagagaa tcatcaggag tttacctgcc aaccagatat gacttgtgat
    42841 gataaaggct gccagctctg cccgcgcggc gtattacccg ttggtgatcg cggcacaacc
    42901 ctgaagtggc ggctggctga ctctcatacg cagtacggta ttttgctggc gaccctgccg
    42961 caggggcgtc atcttagcca tgatcaacaa caactggtgg ataccctggt tgaacaactc
    43021 accgccacgc tggcgctgga tcgccatcag gaacgtcagc aacagttgat cgtgatggaa
    43081 gagcgtgcca ccattgcgcg cgaactgcat gattctattg cccaatctct ctcttgcatg
    43141 aagatgcagg tgagttgttt acagatgcag ggcgatgcgc tgccagaaag cagccgcgaa
    43201 ctgttaagtc agatccgtaa cgaactgaat gcatcctggg cgcagttgcg tgaattgctc
    43261 accacattcc gcttgcagct caccgagcct ggattacgtc cggcgctgga ggcgagttgc
    43321 gaagagtaca gcgccaaatt tggcttcccg gtgaagctgg attatcaatt gccgcctcgc
    43381 ctggtgcctt cgcatcaggc aatccacttg ttgcaaattg cccgtgaggc attaagtaac
    43441 gccctcaaac attcgcaagc gagtgaagtc gtggtgacgg tggcgcaaaa cgataatcag
    43501 gtcaaactga ccgtccagga taacggctgc ggcgtgcctg aaaatgccat ccgcagcaat
    43561 cactacggca tgataataat gcgcgaccgt gcgcaaagtt tacgaggcga ttgccgcgtc
    43621 cgccgtcgtg aatcaggtgg caccgaagtg gtggtcacct ttattcccga aaaaactttc
    43681 acagacgtcc aaggagatac ccatgagtaa tcaggaaccg gctactatcc tgctgattga
    43741 cgatcacccg atgctgcgaa ctggcgtaaa acagcttatc agtatggcac cagatatcac
    43801 cgtggttggc gaagcgagta atggcgaaca gggtattgaa ctggcggaat ctcttgatcc
    43861 cgatctgatc ctgttagatc tcaatatgcc cggcatgaac ggtctggaaa cgctggataa
    43921 actgcgcgaa aagtccctct cagggcgcat tgtggtattc agcgtctcta accatgaaga
    43981 agatgtggtc accgcactga aacgcggcgc ggatggctat ctgttaaaag atatggaacc
    44041 ggaagatctg ctgaaagcat tgcatcaggc agctgctggc gaaatggtat taagcgaagc
    44101 attaacgcct gttctggccg ccagcttgcg cgctaaccgt gccactactg agcgcgatgt
    44161 taaccagtta accccacgcg agcgcgatat tctcaagctg attgcccagg gtttgccgaa
    44221 caagatgatt gcccgccgcc tggatatcac cgaaagcaca gtaaaagtgc acgtcaagca
    44281 catgctgaag aaaatgaagc tcaagtctcg cgtggaagca gcggtatggg tgcatcagga
    44341 gcgcattttc tgattacggt tcccagcgca gttcgtcgtt tgacaatgca tcgaacggtt
    44401 cgacaagcgt tagcgtgatc tcattggagg agacacgctg cccctggtta tcttccacca
    44461 ccaccgacaa tcgccagtga ttgcttgccc cttccccgtt ctgccagtca ggcatgatca
    44521 gcgtccagcc ctccgcgctg ttggcttgtg cgcccggcgt caaacttaat atctgcgtat
    44581 cgccctgcca aatcagttgc cgaataccgt aacgactgcg gatttgtaat ttcagcggca
    44641 ctgtttcgcc aggttttaga tcccacggcg gtgtcgccag aaacaccgtt aacgtttttc
    44701 gctgtcggta ctcaagagtc ggtagattat ttcgctgcgg attgtcatag cgactaccac
    44761 gtaacgactg actttcggca acctcacccg cagaaagttg ttttttgagc ggtacaccaa
    44821 agcggtaatt aagattcagc ccgaggttat tttgattctc gccactttca ccctgtttat
    44881 gctgggccgt cacagtgact aatggcacag gggtgtaatt taatcccaga ctcaacgcga
    44941 cgggattgtg ataacccgta ccagagttaa acaaatcaac acgatcacca aaatactgtt
    45001 ctacgctgac actggtattg aggtgttgat agaacggcat gcgcatccgg gctgtcaggt
    45061 catacccgcg cgccatccgt tgttcctgcg tggctgtctg ttcatgccat gcagcaaacg
    45121 gctgataaaa gtttgccgat agtcgcaaat attcgcccca cgcttcggca ccaaagcccg
    45181 ctcgctgaag attttcgtcc agcaagttgt cataaaaagt gttataaccc accagccagt
    45241 tgccgcgcgc ccagcgttgc ccaacgccca cattgctcac caacccatca tcctgctgag
    45301 taagaccaag ctggctccag gtgagataac gatcattatc ttgtaacggc acaaaccagc
    45361 ttccacgact gccggtgaaa tgtccttcgt tatccacttt gacgtcaaca ctggcatttc
    45421 cccacggtga tagccaggac tctacgtgct gattaacctg ttgactaagc gcgtcgcgga
    45481 cttttcccaa tgcgaaagct tttgcctgct cgccagtatc cagcccgtta tcattcatac
    45541 tggtttcgcc gaaatctttc acaatttcag caaagtgttt ttccccatca tgattttcgg
    45601 gagccatgcc taaatccggc agaccatcat tgttattatc aaagggattt gccgcttttt
    45661 gctcgaaggt agattgtgcg ttagctgtac cgcctgctac aagcaagagt aaataaaacg
    45721 ggataatgcg aggaacgaaa cggctcaaaa cgtcggtaac ttccggtaat aatgaaaaac
    45781 catagcctta aagaataacg attactggca acttttgtcg tattttctcg cgctttcagg
    45841 aaaatcctta aaaatgtgac cgattggtca gacgctgcag aagcagtaag atagaagatg
    45901 aatgtctaat tccctgaata caggagtaaa taatgcaaaa aatcgtgatc gttgccaatg
    45961 gcgcacctta cgggagcgaa tccttgttta acagcttgcg gctggccatt gcgttacgag
    46021 agcaggagag caatctggat ctgcgtctgt tcctgatgtc tgatgcggtc acagccgggt
    46081 tgcgcgggca aaaaccaggg gaaggctaca acattcagca aatgctggag atccttaccg
    46141 ctcagaatgt accggtgaaa ttgtgcaaaa cctgtaccga cgggcgcggg attagcacac
    46201 ttcctcttat tgatggggtg gaaatcggta ctcttgtgga actggcgcaa tggacgctgt
    46261 cagccgataa agtgctcaca ttttaatgtt tgagggggcg tctgacgccc cactatgagg
    46321 gaagtttact caggcgaatc ccggccgtaa cacaccatcc ggataattct ccgccagcag
    46381 tttttttacc gataccagca aatcattcag cccatcgtcc tgcattccca gtttgataag
    46441 ctcctgttcc agtgaaaaca ggtattgtgc gttggttccc agcggaccgc tcgccgccgc
    46501 aatcaacggc gcgatgacct gagcgcgagt gtcagattca tattctggat gtcgcgggtc
    46561 cataataaac acaatggcgt ttactgtgca tccatcatca agatcaagct gacaccaggt
    46621 tggcagataa cagccggtaa tcatctcgcg cttccacaac agggttagtt cctgctccag
    46681 cgtctcttct ggcagtcgat aggcgacgcc tgtagtgcgt ccgccctctt tcagtgcaag
    46741 catccgtccc ggctggtgcg cagtcccgcg cccggcggtc aggcgcaggc aaaacgcgcg
    46801 atgccatcca accagtgtac cggtgcacga ctcggtaaac tccagtgccg gattccacat
    46861 caacgatcca taaccgaaga tccacaccgg tccctcatca ggtcgacaag ccagcgtcgc
    46921 cgccagcgaa gccgcccgtt gctctgctga ccataagagt gattcttcaa tggcaccaaa
    46981 tgccgtttta caatcggcat tcatcaagaa atcacgcgtt atcaccttac acctccgcca
    47041 ctgcatactt ccctgcgaca actttttggt gtcttccgga catttttact gctcattttg
    47101 aaaccactaa cgggacaata tgcaatccac ggaccacttg caaggcaaca ccgcacgaat
    47161 agctttaact aagctaccgg ttttacgatt ttttatgcca tttatcatca tcccctttgg
    47221 catattcatg cttcacagca gcccaggcca ctttatgcgc ggtttcttcg cgactggcgt
    47281 catcacgccg atcgtcttta tctttatatt gatcccatgc gctgttgaac gcttctttat
    47341 agatatcctg ggcatgagac ggtagaacgt gctttacgct ttccggcaga tcgcttttcg
    47401 ttttatacgg catcgttgac tccttttaca aagttgaaca ttaagtgtgg tcaacaatat
    47461 ctactcttgc caaaacataa gaattttcat gtcagactgt tcttaagaaa cgctaaaaca
    47521 aacattgtct aaacgtaata cttattttta tgtcccaatg agtaaaaaat cgacagtggg
    47581 gtaaaaattc gtaaaatgtt accgatatcc agacactttc tgttattttt cctacctatt
    47641 ttttacattt tcgataaatt catctgcaaa gggataacca taatgtcaaa tgctcaagag
    47701 gcggtaaaaa cccgccacaa ggagacttcg cttattttcc cggttctggc gctggtagtg
    47761 ctgttcctgt ggggaagcag ccagacacta ccagtggtca ttgccatcaa tcttcttgcg
    47821 cttattggta ttttaagtag cgcctttagt gttgtccgtc atgcggacgt attagcccat
    47881 cgcctgggag aaccttacgg ttcgcttatt cttagccttt cagtggttat tcttgaagtc
    47941 agtttgattt cagctttaat ggcaaccggc gacgccgcgc caacgctaat gcgtgatacg
    48001 ctctattcaa tcattatgat tgttaccggt gggctggttg gcttttcatt attgttgggc
    48061 ggtcgtaagt ttgccaccca atatatgaat ctgtttggta tcaagcagta tttaattgcg
    48121 ctgttccccc tggcgataat cgtactggta tttccaatgg ctctgcctgc ggcgaatttt
    48181 tcaaccggtc aggcgttact ggtagcatta atttctgcgg caatgtatgg cgtatttttg
    48241 ctgatccaga ccaaaacgca tcaaagtttg tttgtctacg agcacgaaga tgacagtgat
    48301 gacgatgacc cgcatcacgg taaaccgtct gcccatagca gcgtgtggca tgctatctgg
    48361 ttgattatcc atctgattgc cgttattgca gtcaccaaaa tgaatgccag cccgctggag
    48421 acattgctcg acagcatgaa tgcccctgtc gcctttactg gcttcctggt ggctctgttg
    48481 attctgtcgc cggaaggttt aggtgcgtta aaagcagtgt tgaacaacca ggtccagcgc
    48541 gcgatgaatc tgttctttgg ttcagtgtta gcaaccattt cgcttaccgt acctgtcgtc
    48601 acgctaattg cctttatgac gggtaacgaa ttacagtttg cacttggtgc gccagaaatg
    48661 gtggtgatgg tggcctcttt agtgctgtgc catatctcct tctccaccgg acgtactaac
    48721 gtgctcaatg gcgcagcgca tctggcactg tttgccgcct atttgatgac gatatttgcc
    48781 tgacagttat atctcctccg atgacggaaa tcgggggaga taagataaat tcctgcttat
    48841 tgtatttctt catactacct ggacagctcg ttaatcagaa ttgcgagcct gtaccacaac
    48901 ctgcacgaaa ccactgcaac agctgttgaa aagccggaat gtactcttta ttgcaaacat
    48961 ttcagggatg aagtgaagat gaattgagac gtagtatcca caccaagccg gaattatccg
    49021 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg aaagagaaaa
    49081 cccccgcacg ttgagaggta aaacctgaca acaaaacggg ggctaatctt gaaaccagac
    49141 acttcaagaa tagccgcaaa atgttgtcat tacaacaagg cggctatatg acgctcgcgc
    49201 agtttgccat gactttctgg cacgacctgg cagcaccgat cctggcgggg attattaccg
    49261 cagcgattgt cagctggtgg cgtaaccgga agtaaggtga aaacgggcgt caggctgccg
    49321 tgatggcaat atgcgcccga aaccggccgc aggggtaaca gcctgcggcc atttttgtct
    49381 ttatctcttc cacaagtaaa tattggcaac ccgagctgga agcatcatcc agagtactca
    49441 tgaaacgact gtaacagctg ttgaaaaacc ggaatgcact cttcattgca aacatttcag
    49501 ggatgaagtg aagatgaatt gagtcgtagt atccacacca agccggaatt atccggtgag
    49561 gcgcaatgtt gcgggggctt tatccctggt ggcattggtt gctggaaaga gaaaaccccc
    49621 gcacgttgag aggtaaaacc tgacaacaga acgggggcta atcttgaacc atcaacaatt
    49681 caagaatagc cgcaaaatgt tgtcattaca acaaggcggc tatatgacgc tcgcgaagtt
    49741 tgccatgatt ttctggcacg acctggcagc accgatcctg gcggggatta ttaccgcagc
    49801 gattgtcggc tggtggcgta accggaagta aggtgaaaac gggcgtcagg ctgccgtgat
    49861 ggcaatatgc gcccgaaacc ggccgcaggg gtaacagcct gcggccattt ttgtctttat
    49921 ctcttccaca agtaaatatt ggcaacccga gctggaagca tcatccagag ccctcatgaa
    49981 acgactgtaa cagctgttga aaaaccggaa tgcactcttc attgcaaaca tttcagggat
    50041 gaagtgaaga tgaattgaga cgtagtatcc acatcaagcc ggcgttatcc ggtgaggcgc
    50101 aatgttgcgg gggctttatc cctggtggca ttggttgctg gaaagagaaa acccccgcac
    50161 gttgagaggt aaaacctgac aacagaacgg gggctaatct tgaaccagaa caattcaaga
    50221 atagccgcaa aatgttgtca ttacaacaag gcggctatat gacgctcgcg cagtttgcca
    50281 tgattttctg gcacgacctg gcagcaccga tcctggcggg aattattacc gcagcgattg
    50341 tcggctggtg gcgtaaccgg aagtaaggtg aaaacgggcg tcaggctgcc gtgatggcaa
    50401 tatgcgcccg aaaccggccg caggggtaac agcctgcggc cttttttgta tctggcaccc
    50461 atgaaaaaag tcttaacgca gaacgctaag actttatttt tcaagcaaaa aagattactt
    50521 gctggtatcc agttcttcga aacctttcac cagatcatca atcgctttca tctgcttgag
    50581 gaacggttcc agtttagcca gcggcagcgc ggatggacca tcacatttcg catgttccgg
    50641 atccggatgc gcttcaataa acagccccgc cagacctacc gccataccgg ctcgtgccag
    50701 ctcagccacc tgagcacgac gaccaccgga agctgcgcca aacggatcgc ggcattgcag
    50761 tgcgtgggtc acgtcgaaaa tcaccggcga gttaccagac actttcttca taatgctgaa
    50821 gcccagcata tcgacaacca ggttgtcata gccgaagtta gcaccacgat cgcaaagaat
    50881 cactttttcg ttgccgcctt ctttgaattt atcaacgata ttacccatct gtcccgggct
    50941 gacaaactgt ggtttcttga cgttaattac cgcaccggtt ttcgccatgg cttcaaccag
    51001 gtcagtctgg cgagcaagaa acgccggcaa ctgaatcaca tccacgacat cagcaacggg
    51061 ctgtgcctga cttggttcgt gaacgtcggt gataattttc acgccaaaag tctgcttcaa
    51121 ctcctggaag attttcatcc cttcttccag gcccggtcca cgataagagt ggatggagga
    51181 gcggttggct ttgtcaaaag aggctttgaa cacgtaaggg atacccagtt tctgggtcac
    51241 agttacgtag tgctcgcaaa tgcgcatcgc cagatcgcga gattccagca cgttcatacc
    51301 gccaaacagt acgaacggca ggtcatttgc tacgttgatg tcgccaatgc taaccacttt
    51361 ttgtttcata ggatcgcctt aatcttgagt aaaatgtcga ttaattaatg cagcacaata
    51421 tgtttatgcg cgatgttatt tatttgcgca cggatcattt cgctgatcgg gtcttccgga
    51481 cactgttcaa cgaaataact taaatcgttc aacgcaacgt gttcgcaatc cagttgcgca
    51541 taaatcaacc cgcgatcgcg aatttcatag ggatcttcag ggttgaattg taataaagct
    51601 tcgctggtgc gtaacgccag ctccatctga ttttcttcca tcaacgaggc tttgagtgta
    51661 tccagcaatt tgcggattac ctcaatgtta tcagcttcat caaggtcttc ataaaacagt
    51721 tccgccgacg ggctgatatt tccctttaac catacgtcca gcatatgttc gcttaacgat
    51781 tcaccgttaa aaggattaat cagccaaatt tcgccatccg gacattcaat gcgcaatatc
    51841 agctgcgtag ggaaaatcac cggcagcagc ggcaaatcga gacgattcgc gacccataat
    51901 aaaaccgcac ctaatgatac cgcactgccc tgtcgattct ttaacacctg gtccagccat
    51961 aatgcatcgg aaagacgata aacgccgcgt gaggctttaa atccccagtc gccgtaaaac
    52021 agcgcgatca gtttttcctg ctgctcttct aaaggcagaa gctggctgat ttcttccttc
    52081 gccagactaa cgagacgctc cagttcgtcg taaacatctt gcgagggaaa atcgcggcgg
    52141 attgcttcgc aagccaggat catgccttcg cacaatggcg ctttattaaa ttcgaaatca
    52201 gctaacgatc tcatgactta ccccagtaac ggtacttttg tggtggcgag tttaatgatg
    52261 atgtacagca ccaccaacgc cagcgggaag gcaataatgc gcgcctgctg actgtgcata
    52321 cgacgataat cgagtgcaat aaaacccaaa acgatataaa tgataactcc aaacagcttt
    52381 tcagtcagcc actgtgcctg cccggaaaat ggcaggatgt gcgctttagc catcaacgca
    52441 atgccgctta acaataacac cgtatcgaca accggcggca caatgcgcgt ccagcgagca
    52501 aatgcctgtg gatgcttctg ataacgtagc cagaaacgta aggttaatag cccaacagaa
    52561 agcgcgatac taataagatg aacactaagc agtgtagaaa aacttgtcat tgataatagc
    52621 ggccgagcgt tacgcgctcg ttatcaccat agtcacggca ggtttcgacg tcatgatacc
    52681 ccgcgaggat aaatgcttgt cgcaccgctt cgccctgctg ccagccatgt tccagaagca
    52741 gaaagccgcc ggataccagc gcgttacgcg actgttcgat gatatgcacg atgtctgcca
    52801 ttccactgtc tgccgcaacc agcgcagtga gcggctcaaa gcggacatcg ccttgttgaa
    52861 gatgtgggtc ctgctcgtca atatacggcg gattgctgac aatcatcgca aactgctgcc
    52921 cggctagcgc gctaaaccag tcgctttgca gaatgtggat atttttgatc gccagatgct
    52981 gggcattacg ttgtgccagg gagacagcat caggcatacg atctacagcg ataatttcgc
    53041 agtccgggcg ctcgctagcc agcgcaagcg caatcgcccc ggtacccgtc ccgagatcaa
    53101 gaatacggca aggttgttca ggcaaccgcg ccagtgcctg ctccaccaga cactccgtat
    53161 ccgggcgcgg aattaaggtc gctggcgaaa caaataacgg caacgaccag aattctcgca
    53221 ccccggttaa atgagcaatg ggttcaccat cgcgacgacg tgtcagtagc gcatcaagtt
    53281 gctgacattg ttcgtcagtc agctgcgttt caccaaaggc gagaataaaa gtacgccctc
    53341 tgccggtaac atgctccagc aggatttcag catcacgccg cgggctttcg ctcgcctgaa
    53401 gttggcttat tgcttcacgt aaccagtgtt gatattccat tattcctgct cggacaacgc
    53461 cgccagttgg tcggcctgat gttcctggat aatcggttca atcagcatat ccagcttacc
    53521 ttccatcact tcatccaggc ggtagagcgt caggttgatg cggtgatcgg taacgcgccc
    53581 ctgcgggaaa ttgtaagtac ggttacggtc gctgcgatcg ccactcccca gcaggttacg
    53641 acgggtagac gcttcggcct gttggcgttt tgccatttca gcagcgtgga tgcgagcacc
    53701 gagcactgaa agcgccttcg ctttgttttt atgttgtgaa cgttcatcct gacattcaac
    53761 aacaatcccg gtcggcaagt gagtaatacg aattgccgaa tcggtggtgt taacgtgctg
    53821 accacccgcc cctgacgagc ggaaagtatc aatgcgtaaa tctgctgggt tgatgtccgg
    53881 cagttctgcg tcaggcagtt ctggcattac cgcaacggta caagcagaag tatgaatacg
    53941 accctgcgat tccgtagcag gaacacgctg cacgcgatga ccgccagatt caaatttcag
    54001 acgaccatac acaccatcac cgctaatttt ggcgatgatc tctttataac caccatgttc
    54061 accctcgctg gcgctcatga tttctacccg ccagcggcgg gcttcggcgt aacggctgta
    54121 catacggaac agatcgcctg cgaacagcgc cgcttcgtcg ccgccggttc cggctcggac
    54181 ttcgaggaag gcgttacgtt cgtcatcagg atcttttggc agtaacagaa cctgtaattg
    54241 ctgttccagt tgctcgcttt tttctttagc ttcgcgcagt tcatcctgcg ccatctcacg
    54301 catttcagga tcatcgagca tcatctgtgc ggtttcgata tcttcctgaa cctgttgcca
    54361 gtcggtaaaa cagcgcgaaa catcacttaa ctgcgcatat tcgcgtgata atgcgcgaaa
    54421 acgttcctgg tcggcgatag tttgcgcgtc acccagcaac gcctgaactt cttcatggcg
    54481 ttcatgcagg gcttccagtt tggcaacgat agaaggcttc ataggcgtaa atgcaccctg
    54541 taaaaaaaga aaatgatgta ctgctactcc agcccgaggc tgtcgcgcag aatattcagg
    54601 cgttcgttat ccccgtcacg ggcggcctgt tgaagtgatt tcgttggcgc atggatcaag
    54661 cggttagtca gtttccatgc cagatcctgc ataatggctt gcgcgtcgcc gccctgctca
    54721 agggccgcta acgctttggc ggttaactca tcgcgaacgt gctctgcctg gctgcgatac
    54781 tcgcgaatgg tttcgctggc gctttgtgct cgcagccacg ccataaattc gctggtttcc
    54841 tgagcgacaa tagtttccgc ctcaaccgct gcggctttac gctgcgccag gttgtgcgaa
    54901 atgatgcttt gcagatcgtc cacactataa agataagcat tcgccagttt gccaacttcc
    54961 ggctcaacat cgcgcggaac ggcaatatcc accaacagca ttggttggtt gcgacggctt
    55021 tttaatgcgc gctccaccat gcctttcccg ataatcggta acgggctggc ggtggaactg
    55081 atgatgatat cggcttcgcg cagacgttcg tcgatctcac tcagggcaat cacttccgcg
    55141 ccaacttcat ctgccagaat ttgggcacgt tcgcgagtgc ggttggcgat aatcatcttc
    55201 tgtactttat gttcgcgcag atgacgcgct accagctcga tggtttcgcc cgcgcctacc
    55261 agcaacactg tgaccgtaga gagcgattca aagatctgcc gcgccagcgt acaagccgca
    55321 aaagcgacag acacagcact ggcaccgata tctgtttcag tgcgaacgcg tttcgctaca
    55381 gagaaagatt tctggaacat gcgttccagt tcgctggcct tcatatgacc tttttgcgaa
    55441 tcggcaaacg cttttttaac ctgaccgagg atctgcggct ccccaagaac cagtgaatcc
    55501 aggccgctgg caacacgcat taaatggcta accgcgtcgt tatcctgatg ccagtagagg
    55561 cttttacgca gatcttcttc attaagattg tgataatcgc aaagccagcg gattaacgcc
    55621 tcttgcaggt tatcctgctc ttcaacgcta agataaagtt ccgtgcggtt gcacgtcgac
    55681 agcaccacgc cgccctgcac catcggctgc gcaagcaggc tgtcaagcgc ctgatcgagc
    55741 ttatccggcg aaaacgatac acgttctcgc agcgatacag gtgccgtttt atggttgata
    55801 ccgagtgcta aaagggtcat gtctgcggga aataatacca acgttgatag ggttagtctg
    55861 cttgcatcat acaggatgcg taagatcaat aaaagagagc gccccctttt ggagtaattg
    55921 ccgaaagccg ttagattctg gcaattaaga caacttgaac atagacgata gcggacggta
    55981 acgctagcat taagggttat aactgcaacg tatctcaagg acttgtcatc actatgcccc
    56041 tgcccgattt tcgtcttatc cgcctgctac cgctggctgc tcttgtgctc actgcctgtt
    56101 ccgttaccac gcccaaaggt cctggcaaaa gcccggattc gccacaatgg cgtcagcatc
    56161 agcaagacgt gcgcaatctt aatcagtatc agactcgcgg cgcgttcgct tatatttctg
    56221 accaacaaaa agtgtacgcc cgctttttct ggcagcaaac cggccaggat cgctaccgtc
    56281 tgctgctcac taacccattg ggcagcacgg aactggagct gaatgctcaa ccgggtaacg
    56341 tgcagttagt cgacaataaa ggtcagcgtt ataccgccga tgacgccgaa gagatgattg
    56401 gcaaattgac cggaatgcca attccgctca acagcttgcg ccagtggatt ttaggtttac
    56461 cgggtgatgc aaccgactac aaactggacg accagtaccg cctgagcgaa attacctaca
    56521 gccagaatgg caaaaactgg aaggttgttt atggtggtta tgacaccaaa acgcaacctg
    56581 cgatgccagc caatatggaa ctcaccgacg gtggtcaacg catcaagtta aaaatggata
    56641 actggatagt gaaataatgc ggacacagtg gccctctccg gcaaaactta atctgttttt
    56701 atacattacc ggtcagcgtg cggatggtta ccacacgctg caaacgctgt ttcagtttct
    56761 tgattacggc gacaccatca gcattgagct tcgtgacgat ggggatattc gtctgttaac
    56821 gcccgttgaa ggcgtggaac atgaagataa cctgatcgtt cgcgcagcgc gattgttgat
    56881 aaaaactgcg gcagacagcg ggcgtcttcc gacgggaagc ggtgcgaata tcagcattga
    56941 caagcgtttg ccgatgggcg gcggtctcgg cggtggttca tccaatgccg cgacggtcct
    57001 ggtggcatta aatcatctct ggcaatgcgg gctaagcatg gatgagctgg cggaaatggg
    57061 gctgacgctg ggcgcagatg ttcctgtctt tgttcggggg catgccgcgt ttgccgaagg
    57121 cgttggtgaa atactaacgc cggtggatcc gccagagaag tggtatctgg tggcgcaccc
    57181 tggtgtaagt attccgactc cggtgatttt taaagatcct gaactcccgc gcaatacgcc
    57241 aaaaaggtca atagaaacgt tgctaaaatg tgaattcagc aatgattgcg aggttatcgc
    57301 aagaaaacgt tttcgcgagg ttgatgcggt gctttcctgg ctgttagaat acgccccgtc
    57361 gcgcctgact gggacagggg cctgtgtctt tgctgaattt gatacagagt ctgaagcccg
    57421 ccaggtgcta gagcaagccc cggaatggct caatggcttt gtggcgaaag gcgttaatct
    57481 ttccccattg cacagagcca tgctttaagc cgggcaagct gagtttcggt gacaacgtca
    57541 ccttgttcca gacgttgcat cgcgctcttt aatacaccgc ctggaaagga tcatgcctgg
    57601 cccgcacagt tttcggcaga ttctttccac caatggacgc atgcctgagg ttcttctcgt
    57661 gcctgatatg aagctttttg ctggtaacgc caccccggaa ctagcacaac gtattgccaa
    57721 ccgcctgtac acttcactcg gcgacgccgc tgtaggtcgc tttagcgacg gcgaagtcag
    57781 tgtacaaatc aacgaaaatg tacgcggtgg tgatattttc atcatccagt ccacttgtgc
    57841 ccctactaac gacaacctga tggaattagt cgttatggtt gatgccctgc gtcgtgcttc
    57901 cgcaggtcgt atcaccgctg ttatccccta ctttggctat gcgcgccagg accgtcgcgt
    57961 ccgttccgct cgtgtaccaa tcactgcgaa agtggttgca gacttcctct ccagcgtcgg
    58021 tgttgaccgt gtgctgacag tggatctgca cgctgaacag attcagggtt tcttcgacgt
    58081 tccggttgat aacgtatttg gtagcccgat cctgctggaa gacatgctgc agctgaatct
    58141 ggataaccca attgtggttt ctccggacat cggcggcgtt gtgcgtgccc gcgctatcgc
    58201 taagctgctg aacgataccg atatggcaat catcgacaaa cgtcgtccgc gtgcgaacgt
    58261 ttcccaggtg atgcatatca tcggtgacgt tgcaggtcgt gactgcgtac tggtcgatga
    58321 tatgatcgac actggcggta cgctgtgtaa agctgctgaa gctctgaaag aacgtggtgc
    58381 taaacgtgta tttgcgtacg cgactcaccc gatcttctct ggcaacgcgg cgaataacct
    58441 gcgtaactct gtaattgatg aagtcgttgt ctgcgatacc attccgctga gcgatgaaat
    58501 caaatcactg ccgaacgtgc gtactctgac cctgtcaggt atgctggccg aagcgattcg
    58561 tcgtatcagc aacgaagaat cgatctctgc catgttcgaa cactaatcga acccggctca
    58621 aagacccgct gcggcgggtt tttttgtctg taatatccat ttgtatgacc tatgcctcct
    58681 tcacctgcca tttagttgac agatgatgcg ctcacggatg aaacattatt gtgaacaaaa
    58741 tattttcctc acatgtgatg cctttccgcg ctctgatcga cgcttgctgg aaagaaaaat
    58801 atactgccgc acggtttacc cgtgacctga ttgccgggat aaccgtcggg attattgcta
    58861 tcccgctggc gatggcgttg gctattggta gtggtgtggc accccagtac ggtttatata
    58921 ccgcagctgt tgcggggatt gtcattgctc tgacgggtgg gtcacgcttt agcgtttccg
    58981 gtccgactgc ggcatttgtg gtaattctct atcccgtgtc gcaacagttt ggactggcag
    59041 gactgctggt tgcgaccttg ctgtcgggga tttttttgat tctgatgggt ctggcacgct
    59101 ttggtcgcct gattgagtat attccggttt ccgtcacctt aggtttcacc tcgggtatcg
    59161 ggatcaccat cggtaccatg cagattaaag attttctcgg tctgcaaatg gcccatgtcc
    59221 cggaacatta tctacaaaaa gtcggcgcat tatttatggc gctgccgacc attaatgtgg
    59281 gtgatgctgc cattggcatt gtgacgctag gtattcttgt tttctggccg cgtctgggca
    59341 ttcgtttacc cggtcacctt ccggccttgc tggctggttg cgcggtgatg gggattgtta
    59401 acctgctcgg cggacatgtt gctaccatcg gttcgcaatt ccactacgtc ctggccgatg
    59461 gttctcaggg taacggtatt ccgcaactgc tgccgcaact ggtgctgccg tgggatctgc
    59521 ctaattcaga attcacgcta acctgggatt ctattcgcac actgctgcct gcggcattct
    59581 caatggcaat gctcggcgca atcgaatctc tgctctgcgc cgtggtactg gatggtatga
    59641 ccgggacgaa acacaaggcg aacagcgaac tggttggaca gggactgggg aatattatcg
    59701 ctccgttctt tggtggtatt accgctacag ctgccatcgc gcgttctgcc gctaacgtcc
    59761 gtgccggggc aacttcccct atctcagcgg tgatccactc tattctggtt attcttgccc
    59821 tgctggtact ggcaccgctg ctctcctggc tgccgctttc cgctatggca gccctgctgt
    59881 tgatggtggc gtggaacatg agtgaagcgc ataaagtggt cgacttgctg cgtcatgcac
    59941 cgaaagatga catcattgtc atgctgctgt gcatgtcgct gaccgtgctg tttgatatgg
    60001 ttattgccat cagcgtgggg atcgtgctgg catcgctgct gtttatgcgt cgtatcgcac
    60061 gtatgactcg cctggcaccg gtagtcgtag atgttccaga cgatgttctg gtactgcgcg
    60121 ttattggccc gctgtttttt gctgctgctg aaggcttgtt cacggacctg gagtcacgtc
    60181 ttgaaggcaa acggattgtg attctgaagt gggatgcggt tccggtactt gatgctggtg
    60241 gtcttgatgc gttccagcgt tttgtgaagc gtctgcccga gggatgtgaa ctgcgcgtgt
    60301 gcaacgtgga attccagcca ctgcgcacta tggctcgcgc tggcattcaa ccgatcccgg
    60361 gacgcctggc gttcttcccg aatcgtcgcg cggcgatggc ggatttataa gtacgagatt
    60421 gaccagtcag cgcaaactga ctggtcagca aactgcatta tttgttagct atgacggcga
    60481 ttatcgctac ggcggcaacg tttgtcatag tggcgaaccc accagtaacg gtcgcacacc
    60541 tgttcatgcc cgccaacacg ggcacccgcc agccagagaa tggcaccgac gaaaatactt
    60601 agcactgcac catgtgcgaa atactggggc atattaaact gtggtaactg gttgaggatt
    60661 gaatacccca cgccgaccac cattaccacc agacccaacc ccatgagcac gttaccgagt
    60721 aacgaagcgt ttttgcgttt catatgtcac ctccggaact ttctgggttg taacagggaa
    60781 tacccctctt ccttatgtgt aaagtataga caacaccctg tgggttatgt gcgggcgtga
    60841 tcacaattac aacccttatt tcaacaaaac tttacaaata aacgcctgaa caccttgact
    60901 ttctaatagt ccactcaggc aattattacg ttatctgcat ttgatacgca gttttttgtc
    60961 aagcggggcc gcaaccagta aactacgcgc cagttatgta cacactcagg acaaaaaaac
    61021 gtgacgatta aattgattgt cggcctggcg aaccccggtg ctgaatacgc cgcaacgcga
    61081 cataatgctg gtgcctggtt cgttgactta ctggcagagc gtttgcgcgc tccgctgcgc
    61141 gaagaggcta aattctttgg ttatacttcg cgagtcactc ttggaggcga agatgtccgc
    61201 ctgttagtcc cgactacatt tatgaatctc agcggcaaag ccgttgcggc gatggccagt
    61261 tttttccgca ttaatccgga cgaaattctg gtggcccacg acgaactgga tctgcctcct
    61321 ggcgtcgcca aatttaaatt gggcggtggc catggtggtc acaatggact gaaagacatc
    61381 atcagtaaat tgggtaataa ccctaacttt caccgtttac gcatcggaat cggtcatccg
    61441 ggcgataaaa ataaagttgt cggttttgtg ttaggcaaac cgcctgttag tgaacagaag
    61501 ttaattgatg aagccatcga cgaagcggcg cgttgtactg aaatgtggtt tacagatggc
    61561 ttgaccaaag caacgaaccg attgcacgcc tttaaagcgc aataagtcgt tgtctgcggc
    61621 atttttgccg agtgccgtgt ataataggca aagttatttc catttctgca atctgttagc
    61681 aataacaggt tgattattaa gatattaagg tgatttaaat catgggattc aaatgcggta
    61741 tcgtcggttt gcccaacgtc gggaaatcta ccctgttcaa cgcgctgacc aaagccggta
    61801 ttgaagcggc caactttcca ttctgcacca ttgagccgaa cacaggcgtc gtaccaatgc
    61861 ctgaccctcg cctggatcaa ctggctgaaa tcgtaaaacc gcaacgtacg cttcccacga
    61921 ccatggaatt tgtcgatatc gccggtctgg taaaaggcgc atcgaaaggc gaaggtctgg
    61981 gtaaccagtt cctgaccaac atccgtgaaa ccgaagcgat cggtcacgtt gttcgctgct
    62041 ttgaaaatga caacatcatt cacgtttccg gcaaagttaa cccggctgac gatattgaag
    62101 ttatcaacac cgaactggcg ctggcagacc tcgacacctg cgaacgtgcg attcatcgcg
    62161 tacagaagaa agccaaaggt ggcgataaag acgcgaaagc tgagctggcg gtcctggaaa
    62221 aatgcctgcc ccagttggaa aatgcaggta tgctgcgcgc gctggattta agcgctgaag
    62281 agaaagcggc tattcgttac ctgagcttcc tgacgctgaa accaacaatg tacatcgcca
    62341 acgtcaacga agacggtttt gaaaacaacc catatcttga ccaggtgcgt gaaatcgcgg
    62401 cgaaagaagg ttctgttgtg gttccggttt gtgctgctgt tgaagcagac attgccgaac
    62461 tggacgacga agaacgtgac gagtttatgc aggagcttgg gctggaagag ccgggcctga
    62521 accgtgtgat ccgtgccggt tataagctgc tgaacctgca aacttacttc accgctgggg
    62581 tgaaagaagt gcgtgcatgg accattccgg ttggagcaac cgcgccgcag gcagcgggca
    62641 aaatccatac tgattttgaa aaaggcttta tccgtgcaca aaccatctcg tttgaagatt
    62701 tcatcactta caaaggtgaa caaggcgcga aagaagcagg caaaatgcgt gcagaaggta
    62761 aagattacat cgtgaaagat ggcgatgtga tgaacttcct tttcaacgtc taattaattt
    62821 attgtctcat gaggtttcgc taaatctcat gaagagcgca aaagtatata aaatccacgc
    62881 aattgcgtgg atttttcatt tcaccgagtc ctaatcattc ttgtaacatc ctacactttt
    62941 gtgcttattg cctacagtga accacgagaa taattaaaga catcagtgtc agatgacgga
    63001 tatctcatta gtagtgatat aacgtaagtt ttggtccact acacatcacc cccttcacag
    63061 ctgatcaata ttgatcacgc tccaatatgc tggcgagaaa aggacttacc tgaagggtaa
    63121 tatcccgcag ataaagtccc cgaataacgt gattacgtgc gatcctggca agatgaaaat
    63181 gataaccagt agcacaataa caacgatagg ccagaaaacc cattgaccaa aaatgaatat
    63241 cgatgttttt tgctttttct tattagtaat gcaaaaatgg cgcatattgt ttttgttatg
    63301 cagatacatc aagcgaaatt cccataacta attaggatta ttacaaaaaa tcttcaccaa
    63361 attttttaac cgctgtttgt tgtaatattt ttgccatatt tatagtaacg aattggaata
    63421 agcaacgaca acttagggaa taactcgata taaacccacc tttgtaaggg ggatgttaaa
    63481 tatatattaa ttcctgtgtt cctgattttg cccggtaata gagaattatt ctctgagaag
    63541 ctcatcatga ttgcaccata caaatatcat gatgataaga ggatattatt cattgattta
    63601 aacaaaatga caaaatccat agacccatag tctgggtact atataggagt ggtatgggca
    63661 tcaaacaaca caatgggaat accaaagcag atcgtctcgc tgaattaaaa atccgttcgc
    63721 cctcaattca actgataaaa tttggcgcta ttggtttgaa tgcaattatc ttttcccccc
    63781 tgctgatagc tgctgataca ggaagtcaat atggcaccaa tattactatt aatgatggtg
    63841 acagaattac aggagatacc gccgatccat caggaaacct ctatggtgta atgaccccag
    63901 caggaaacac gcctggcaat atcaacctgg gtaatgatgt caccgtcaat gtcaacgacg
    63961 cctctggata tgcaaaagga atcattattc agggcaaaaa cagctccctg acagctaacc
    64021 gactcacagt agatgttgtt ggtcaaacct ctgccatcgg cattaactta attggtgact
    64081 atacccatgc tgacttaggc acaggcagca ccattaagag taacgatgac ggcatcatta
    64141 ttgggcatag ctcaacacta acagccactc aattcaccat tgaaaactcg aacggtatag
    64201 gcctaaccat caatgactat ggcaccagtg tcgatcttgg aagcggaagt aaaatcacga
    64261 ccgatggaag tacaggtgtt tatatcggtg gtctcaacgg caataacgcc aatggtgctg
    64321 cgcgttttac ggctacagac ctgacaatcg atgttcaggg ctacagcgcc atggggataa
    64381 acgtacagaa aaactctgtt gtcgatctcg gaacaaacag ttccattaaa accaatggcg
    64441 ataatgcaca cggcctctgg agctttggcc aggttagcgc gaatgcactc actgttgatg
    64501 taactggagc cgcggccaat ggcgtcgaag ttcgtggtgg tacaaccact atcggtgcag
    64561 atagccatat ttcttccgcg cagggcggtg gcctcgtcac cagtggttca gacgcgacaa
    64621 tcaatttttc tggcacggcg gcgcaacgaa acagcatctt ttccggcggt tcttatggtg
    64681 cctcggccca gacggcaacg gctgttgtca acatgcaaaa taccgatatt acagttgatc
    64741 gtaatggcag tctggcgctg ggtttgtggg cgctcagcgg cggtagaata accggagaca
    64801 gtttggctat caccggcgcg gcaggagcca ggggaattta tgccatgacc aacagccaga
    64861 tcgacctcac gagcgatctg gtcattgata tgagtacacc cgaccagatg gccattgcaa
    64921 cgcaacatga cgatggttat gccgccagcc gcatcaacgc ctcgggtcgt atgcttatca
    64981 acggtagcgt tctttccaaa ggtgggctaa tcaatctgga tatgcaccct gggtcggttt
    65041 ggacaggttc ctccctcagc gataatgtca atggcggaaa actggacgtt gcaatgaata
    65101 acagcgtctg gaacgtaaca agtaattcta atctcgacac gctggcgctg agccattcaa
    65161 ctgtcgattt tgccagccac gggtcaactg ccggcacatt tgccacatta aacgtagaga
    65221 acctgagcgg taacagtacc tttattatgc gtgctgatgt tgttggcgag ggtaatggcg
    65281 ttaataataa aggggattta ttgaatatca gcgggagtag tgctggtaat cacgtattgg
    65341 ctatccgcaa ccagggcagc gaggccacaa cgggaaatga agttctgaca gtggtaaaaa
    65401 ccactgacgg cgcggcctcg ttcagcgcgt cttctcaggt tgagttgggg ggatatctgt
    65461 acgatgtgcg taaaaatggc actaactggg agctttacgc ttccgggaca gttccggaac
    65521 cgactcctaa tcctgaaccc acaccagctc ccgctcagcc tcccatagtc aaccccgatc
    65581 ctacgcctga acccgctccc acgcctaaac ccaccacgac cgcagatgct ggcggcaatt
    65641 atctcaatgt cggttactta ttgaactatg ttgaaaaccg tacgctgatg caacggatgg
    65701 gtgacctgcg aaatcagagt aaagacggta atatctggtt gcgcagttat gggggaagcc
    65761 tggactcctt tgccagtggc aaactgagcg gctttgacat gggttacagc ggtatccagt
    65821 ttggtgggga taaacgtctc tctgatgtaa tgccgttgta tgtcggtctg tatattggct
    65881 caacacatgc atcgccggac tatagcggag gcgacggtac cgcacgttca gactacatgg
    65941 gaatgtatgc cagttacatg gcacacaacg gtttttacag cgatctcgtt gtaaaagcat
    66001 cgcgccagaa aaatagtttt cacgtactgg acagtcagaa caacggcgtt aaagccaacg
    66061 gcactgcgaa tggattgagc atctccctgg aagccgggca gaggttcaac ctgaccccta
    66121 ctggttatgg gttctatata gagccgcaaa cccagctcac atacagccac cagaatgaga
    66181 tggctatgaa ggcgagtaat ggtctcaata tacatctgaa tcactacgaa tcgctgctgg
    66241 ggcgtgccag catgatactg ggatatgaca tcaccgcagg caacagccag ctgaatatgt
    66301 atgtgaagac tggagctatc cgcgagtttt caggggatac agactatctg ttgaacaact
    66361 cccgggagaa gtacagtttc aaaggtaatg gctggaataa cggcgtggga gtcagtgcac
    66421 agtataacaa acagcacaca ttctacctcg aagcggatta cacgcagagt aacctctttg
    66481 atcagaagca agtcaacgga ggatatcgct tcagctttta agtgcgggat agaccagccc
    66541 ttaaacgatg ctgaaaaatg ttagcgtgcg ccggaatgaa atatagatca ctctgatgta
    66601 tcagaaatcg tctctgtctt tcatacccgg cgcttaaact gccctgcatc aatgccatgt
    66661 tgcttcattt tccgccacag cgtagtgcgg ccgatcccaa gtaaagccga catttcctga
    66721 atgcgaccgc ctgtgacctg ggctgcgtta ataattgcct ctttttcaac ttccgcaaat
    66781 gacagactgg tggaaaggcg tgtggcgctg acatcatctg ttgcctgctc ggtaaacaga
    66841 tgttccggca aatcactgac gcgaatgcgc ccattatcac tactcagagc aagattctcg
    66901 atgacgctgt aaagttcaaa atcgttgcca ggccatgcac aggaaaccag gcgagcgagg
    66961 gcatcgtcat caattttcag ccgcgtagag aagcgttttt caagactgcg taatttgtta
    67021 ttcaccagcg ccggaatgct gccacgccgc atacgcagag gcgggatggt aatttcaaat
    67081 gcatgcaacg cgtaatacag ctggcgacta aaacgatttt gttccaccag cattgcgagg
    67141 tccgcggtcg ttgtggcaat cactttgaca tcaactggta ttaaacgccg cgcatccagt
    67201 cgcgtgataa caccctgctt gataacctga agcaaagcag actgtaactc caccgccaga
    67261 tattcaatct tttcaagaaa cagcgtgccg ccgtgcgcca gttccagccg actcagacgg
    67321 ccattttcat tgtccgtgcg atcgccacca ataaattctt ccgccagcgc agcatcacca
    67381 tataactcac aattgacggc gatataagga cctgcagcac gctcgctttc attatgaatt
    67441 gcctgactta gcagtgcctt gcccacgccc tcttctccac aaagcaggac aggaaagcta
    67501 ctgcgcgccg cctggcgacc aaaatgaatc aagcggcggg tttgcggatc gtcctgtggc
    67561 atatgagcaa aggtatggct gacttttcct aattgactgg tcatcaactg ccgcatctgt
    67621 tccacaggat ggagcaacaa aataaagctg gttccctgcg tttctattat cggtttaagg
    67681 gttatcaccg catcaataaa ctggtgctgg ctttcaaagg ttgcttctac gtgtttgagc
    67741 ggatgtgcct gttttattgc ttgttgcaat acggcgggta acgttaagag ttcagtgatt
    67801 gcccgtccct gacttgccgt cgcgtcaagg cgcaagaccc gcgccgcctg ggcattaata
    67861 aattgcaaat taccctgctc gtcccagcta atcacgccat catccatact ttctaacagg
    67921 gcattaagtt gatttaaatg acggttagtt tcagcgagca aactgtccgt cagcagtaaa
    67981 ttaccgacct cgcgggcgat tgccaacgtc aacggcaaat cagctgcggt agtttgctca
    68041 accggacacg ccagcgctat tgttcccgtc aatcggccct tgctgtcaaa caacggcgtt
    68101 gcacaaaagg cccagttcca gagtgcctgt ttgaaatgtt gatcggccat cgttttcacg
    68161 gcctgaccag agatagccgc taacgatagc gcacaagtac caataattcc ctcggcgcaa
    68221 tacgtgccgt cattgaatcc cagtgcactt agctgctgca aggtttgcgg atcgccatta
    68281 cggctgagaa tgcaggcggt ttcatcgagg ataagcagcg cacactctcg cggtgccata
    68341 tattcccagg catcttccag cgcagcctga ccgagcgtca gcatcgcttt cttacgccga
    68401 taaatagaag caaatgtcac gccctgggcc tgatgtggta cgttccatgt ctcccgtttc
    68461 atcagcttat tgcatcgctc ccaggaggtt gcaattaagg gagatatgcc ccgaccatcg
    68521 ttgttaaaag cgccactcat atcccgcatc ccttatgaca aaaggtgttc cggctcgcaa
    68581 ttttaagtat taatacacgt aaaagtttca atatggaaca ttcattaaag catatgtttc
    68641 actatgaaac ataatttggg ctaattttct tttcccttgc ctgatgcaca atggattcat
    68701 cggcagtcag tggtcgccgt gtcgttgaac atcatccatg ccctaccgta attgctggag
    68761 caaaataatg aaaaaattga tcaatgatgt gcaagacgta ctggacgaac aactggcagg
    68821 actggcgaaa gcgcatccat ctctgacact gcatcaggat ccggtgtatg tcacccgagc
    68881 tgatgccccc gttgcaggaa aagtcgccct gctgtcgggt ggcggcagcg gacacgagcc
    68941 gatgcactgt ggttatatcg gtcaggggat gctttcgggg gcctgtccgg gcgaaatttt
    69001 cacctcaccg acgcccgata aaatctttga atgcgccatg caaattgatg gcggcgaagg
    69061 tgtactgatg attatcaaaa attacaccgg cgatattctt aactttgaaa cagcgaccga
    69121 gttactgcac gatagcggcg taaaagtgac cactgtggtc attgatgacg acgttgcggt
    69181 aaaagacagt ctttatactg ccgggcgacg cggcgttgcc aacaccgtat taattgaaaa
    69241 actcgtaggc gcagcggcgg agcgtggcga ctcactggac gcctgtgcgg aactggggcg
    69301 taagttgaat aatcaaggcc actcaatagg tatcgctctc ggtgcctgta ccgttcctgc
    69361 cgcgggcaaa ccttctttta ccctggcgga taatgagatg gagtttggcg tcggcattca
    69421 tggtgagccg ggtattgacc gccgcccctt ctcttccctt gatcaaaccg tcgatgaaat
    69481 gttcgacacc ctgctggtaa atggctcata ccatcgcact ttgcgtttct gggattatca
    69541 acaaggcagt tggcaggaag aacaacaaac caaacaaccg ctccagtctg gcgatcgggt
    69601 gattgcgctg gttaacaatc ttggcgcaac tccgctttct gagctgtacg gcgtctataa
    69661 ccgcctgacc acacgttgcc agcaagcggg attgactatc gaacgtaatt taattggcgc
    69721 gtactgcacc tcactggata tgaccggttt ctcaatcacc ttactgaaag ttgatgacga
    69781 aacgctggca ctctgggacg ccccggtcca caccccggcc cttaactggg gtaaataagg
    69841 agaaagcaat gtcactgagc agaactcaaa ttgttaactg gctcactcgt tgtggcgata
    69901 ttttcagcac cgagagcgag tatcttaccg gactggatcg cgaaattggc gacgctgacc
    69961 acgggctaaa tatgaaccga ggctttagca aagtggtgga aaaactccct gctatcgcag
    70021 ataaagatat cggtttcatt ctcaagaata ccggtatgac gctgctttcc agcgtcggtg
    70081 gtgccagtgg tccgctgttc ggtaccttct ttatccgcgc cgcacaggcg acccaggcac
    70141 ggcaaagcct gacactggaa gagctttatc agatgttccg cgatggcgcg gacggcgtaa
    70201 tcagtcgcgg gaaagccgaa cctggcgata aaaccatgtg tgatgtgtgg gtgccggtgg
    70261 tggaatcgtt acgtcagtcc agcgagcaaa atctctctgt tccggtggcg ctcgaagctg
    70321 ccagtagaat cgccgaatcc gctgcacaaa gtacgattac gatgcaagcc cgcaaaggcc
    70381 gcgccagtta tctcggtgaa cgcagtattg gtcaccagga tcccggcgcg acctcggtga
    70441 tgtttatgat gcaaatgttg gcgttagccg caaaagagta aggaattggt gatggtaaac
    70501 ctggtcatag tttcacatag cagccgactg ggagaaggtg tcggtgaatt agcccgtcag
    70561 atgttaatga gtgatagttg taaaatcgcc attgccgcgg gaattgacga tccacaaaat
    70621 cctattggta ccgatgccgt caaagtgatg gaggccatcg aatctgttgc tgatgccgac
    70681 catgtgctgg tcatgatgga tatgggtagc gcattattga gtgctgaaac tgcgctggaa
    70741 ttgctggctc ccaagatcgc cgcaaaagta cgtttgtgtg ctgcgccgtt ggtcgaaggt
    70801 acactggcag caacggtcag cgcggcctcg ggagcggata tcgacaaagt tatctttgac
    70861 gctatgcatg cgctggaagc caaacgtgaa caactgggtt taccgtcctc cgacactaaa
    70921 atctctgaca catgtcctgc gtacgatgaa gaagcccgtt ctctggcggt ggtcataaaa
    70981 aaccgtaacg gcctgcatgt acgtccggcc tcccggctgg tttatacctt atcgacattt
    71041 aatgccgata tgttgctgga aaaaaacggc aaatgcgtca caccagagag tattaaccag
    71101 attgcgttac tacaagttcg ctataacgat acgctgcgcc tgattgcgaa agggccagaa
    71161 gctgaagagg cactgatcgc tttccgtcag ctggctgaag ataactttgg tgaaacggag
    71221 gaagtcgctc cacctactct gcgtcccgtt ccgcctgttt cgggtaaagc cttttattat
    71281 caaccagttt tatgtacggt acaggcaaaa tcaaccctga ccgtggaaga agaacaagat
    71341 cgattacgcc aggctattga cttcacgtta ttagatctga tgacgttaac agcgaaagca
    71401 gaagccagcg ggcttgacga tattgccgca atcttttctg gtcaccatac actgttagat
    71461 gatccggaac tgctggcggc ggcaagcgaa ctccttcagc atgaacattg cacggcagaa
    71521 tatgcctggc agcaagttct taaagaactt agccagcaat accagcaact ggatgatgaa
    71581 tatctacaag ctcgctatat tgatgtggac gatcttctgc atcgcaccct ggtccacctg
    71641 acccaaacga aagaagaact cccgcagttt aactcgccaa ctattctact ggcggagaac
    71701 atttatcctt ccacagtact gcaactggat ccggcggttg taaaaggtat ctgccttagc
    71761 gccggaagtc cggtatccca cagcgcccta atcgcccgtg aactggggat tggctggatt
    71821 tgcaagcagg gtgagaaact gtatgcgata caaccagaag aaacgctaac gctggacgtt
    71881 aaaaggcaac gtttcaaccg tcagggttaa gccactgttc ggatggcatc gttctgatgt
    71941 catccggcaa tttactcagc actaatggtt tttaactacc tgttatattt ttcaccaatt
    72001 tatgatgatt tttcttcgct aatcacgaac ccaacgccgc tggctggcta ataaacagac
    72061 gttatttgat cactctattt cgccgttatt tatcccttta attccctttt ctaaaatgcc
    72121 tgacagttcg cagaatgaga tttcgatcat gcagctagtg cgatcctgaa ctaaggtttt
    72181 ctgatacttg aataccgttt tattcggttt cgccaaagga gaatgattga tgaaatcccc
    72241 cgcaccttct cgcccgcaaa aaatggcgtt aattccagcc tgtatctttt tgtgtttcgc
    72301 tgcgctatcg gtgcaggcag aagaaacacc ggtaacacca cagccgcctg atattttatt
    72361 agggtcgctg tttaatgatg tgcaaaacgc caaacttttt ccggaccaaa aaacctttgc
    72421 cgatgccgtg ccgaacagcg atccgctgat gatccttgct gattatcgga tgcagcaaaa
    72481 ccagagcgga tttgatctgc gccatttcgt taacgtcaat ttcaccctgc cgaaagaagg
    72541 cgagaaatat gttccgccag aggggcagtc actgcgcgaa catattgacg gactttggcc
    72601 ggtattaacg cgttctaccg aaaacacaga aaaatgggat tctctgttac cgctgccgga
    72661 accttatgtc gtgccgggcg gacgttttcg cgaggtatat tactgggaca gttacttcac
    72721 catgttagga cttgccgaaa gcggtcactg ggataaagtc gcggatatgg tggccaattt
    72781 tgctcatgaa atagacactt acggtcatat tcccaacggc aaccgcagtt actatttaag
    72841 ccgctcgcaa ccgcccttct ttgccatgat ggtagagtta ctggcgcagc atgaaggcga
    72901 tgccgcgttg aagcaatacc tgccgcaaat gcaaaaagaa tatgcttact ggatggacgg
    72961 tgttgaaaac ctgcaagccg gacaacagga aaaacgcgtt gtcaaacttc aggatggtac
    73021 ccttctcaac cgctactggg acgatcgcga tacgccacga ccagagtcat gggtggaaga
    73081 tattgccacc gccaaaagca atccgaatcg acctgccact gaaatttacc gcgacctgcg
    73141 ctctgccgct gcgtctggct gggatttcag ctcgcgctgg atggacaacc cgcagcagtt
    73201 aaatacctta cgcaccacca gcatcgtacc ggtcgatctg aacagcctga tgtttaaaat
    73261 ggaaaaaatc ctcgcccgcg ccagcaaagc tgccggagat aacgcgatgg caaaccagta
    73321 cgaaacgctg gcaaatgccc gtcaaaaagg gatcgaaaaa tacctgtgga acgatcaaca
    73381 aggctggtat gccgattacg acctgaaaag tcataaagtg cgcaatcagt taaccgcggc
    73441 cgccctgttc ccgctgtacg tcaatgcggc agcgaaagat cgcgccaaca aaatggcgac
    73501 ggcgacgaaa acacatctgc tgcaacccgg cggcctgaac accacgtcgg tgaaaagtgg
    73561 gcaacaatgg gatgcgccaa atggctgggc accgttacag tgggtcgcga cagaaggatt
    73621 acaaaactac gggcaaaaag aggtggcgat ggacattagc tggcacttcc tgaccaatgt
    73681 tcagcacacc tatgaccggg agaaaaagct ggtggaaaaa tatgatgtca gcaccaccgg
    73741 aacggggggc ggcggtggcg aatatccatt acaggatggc tttggctgga ccaatggcgt
    73801 gacgctgaaa atgctggatt tgatctgccc gaaagagcaa ccgtgtgaca atgttccggc
    73861 gacgcgtccg accgttaagt cagcaacgac gcaaccctca accaaagagg cacaacccac
    73921 accttaacca gcgcttactc cgacaacgtc attcggcttc tccggatggc gttgtcggca
    73981 gataacacca tagctatggt gttttctaca cagacggttt ttcaagtcct ggcgggaagt
    74041 attgcgcaaa tgtttgccat acgtggttgc cgaagatagc gtcatttcta cgggtaagtg
    74101 tttcactatc atcccccggt gatttatacc gacgttgcct atataaagca tcgattgcta
    74161 cctgaatatt gttacggtgt tcactcatca acgttttttt gctttctgga gttaaattaa
    74221 taatcacata atccagacac tccagagaat ttagcaacca aaaaaagaat tcagaccatt
    74281 cctcttcgcg taactgaagc attgccgaac gggtattttg gcttaatctg caagaaggat
    74341 tgatcaattc taaatgttca ataagcgcag ccaaaaccgc ctcactatcc gccccatttt
    74401 taacgcctga ctggctactg tctaaaatat ttattttctg aatcacacca aaaacaacga
    74461 aacaataatt aaaaaaggat tctgttgttg ttaccgggaa ccgactttat cggatatcga
    74521 agtgctatat tgattttttt cacccgtaaa gcaggtgtcg agtatacgtt ctgatgtgaa
    74581 agcggatagc ggcatctgta tcgctatccg ccttatgaaa atcttattct cttcgcagga
    74641 ggcggaatat gcccagaacg agaatagctc ccaccaccgc caccaggaag ctgtgcagat
    74701 taaaaccact gatggagccg ccaatgccaa acatggtcgc cagccagccg ccgaccaccg
    74761 caccgactat cccgagaata caggtcagga aaaatccacc accatcacgc cccggcatga
    74821 ttagcttggc gataatgccg gctatcaggc caaaaataat ccaggcaata attcccatgt
    74881 tcaggccctc gtttcgatga atcggacgca agaaatgctt acgtcaacgc tctaagtata
    74941 ggcaagggcg ggatcgatgc tgggcgtatg tcggctgatt taagaacaac atgtaaaaaa
    75001 tattcacgca tttgtattaa gttttgttaa ctgtgaccga taaaccaaag acagtttatc
    75061 agtcaggagt ttttccgcgt gagtcattac catgagcagt tcctgaaaca aaatccgtta
    75121 gccgtcctgg gcgtgttacg cgatttgcac aaagccgcaa ttcctttgcg tctcagttgg
    75181 aatggcgggc agctgatcag caaaatactg gcaataaccc cggataaact ggtgctggat
    75241 ttcggcagtc aagccgaaga caacatcgcc gtgctaaagg cacagcacat taccattacc
    75301 gccgaaactc agggtgcgaa agtcgagttt actgttgaac aactacagca gagtgaatac
    75361 ttgcagcttc cggcatttat caccgtaccg cctcccacct tatggtttgt acaacgacgc
    75421 cgatatttcc gcatctccgc cccactccat ccgccttatt tttgccagac caaactggcg
    75481 gataacagta cgttacgttt ccgcctgtat gatttgtcgt taggcggcat gggcgcatta
    75541 ctggaaacag caaagcctgc cgaattacaa gaaggcatgc gcttcgctca gattgaagtc
    75601 aacatgaggc aatggggtgt ttttcacttt gacgcccagt taatctccat cagcgagcgc
    75661 aaagtgattg atggcaagaa tgaaaccatc accactcccc gtctgagctt ccgttttctt
    75721 aacgtcagcc cgacggtgga gcggcaatta cagcggatta ttttctctct cgagcgagaa
    75781 gcccgggaaa aagcggacaa agtgcgcgac tgatttacat cgcgtccagt gcctgctcaa
    75841 gtttgtagat atagcgcgga gcctgcggcg cagggtgatt tcgcgctacg tgatcgagga
    75901 actcgtcagc atccaaatcg ttgattttgc tgatcgcctt tttccgatct gacgagaaag
    75961 tccgtagcag cgcacctgcc ccgttagcgt atgacaccac cagcgcatat tgcagtacct
    76021 tcggatcttc aatgcctgcc agcgggccgg tttccagaat attcaggtaa gccgccccca
    76081 ttgaaatatt acgctctgga tttttcagct cgctggtcgt cggctcacca ctccagccca
    76141 tacggcgata aacatcacgt ccggaggttg aagcttttaa ctgcatcaaa ccaatggcat
    76201 tcgatttact caccgcattc ggattaccac ccgattcgat agcgataatc gccgtgatca
    76261 attgtggatc gacgccccag gctgcaccgg ctttctggct tattggcatc cactgcatcg
    76321 cacgttgcac cggaactttc gcgttccacg gcgggttcgt atagtcatgc tttgatgaac
    76381 aacccgctaa taacacaatc aaaaaggcaa accatctcaa tttcacttct tctatcctta
    76441 tagcctggaa tcgccgttga ggtgacaacg gcacacctaa gcggcatgat atacatttcg
    76501 tttctgttgt cagcaaggaa ttgccatgtc tctgtttcac ttaattgccc catcgggtta
    76561 ctgcattaaa cagcacgccg cgctgcgtgg tatccagcgc cttaccgacg aggggcatca
    76621 ggtcaataac gtcgaggtta ttgcccgtcg ttgtgagcgt tttgcgggta cggaaacgga
    76681 gcgcctggaa gatctcaatt cccttgccag actgaccacc cccaacacca tcgtgctggc
    76741 tgtacgcggc ggttacggtg ccagtcgttt actggcagat attgactggc aggcactggt
    76801 tgctcgccaa caatatgacc cgttgctcat atgcgggcat agcgatttta ccgccattca
    76861 gtgcggtctt ctggcgcaag gcaatgtcat cacctttagc ggccccatgc tggtagcaaa
    76921 ctttggcgcg gatgaactga acgcctttac cgagcaccat ttctggctgg cgttacgcaa
    76981 tgaaaccttc accattgaat ggcaagggga aggcccgaca tgtcagaccg aaggcacgtt
    77041 gtggggaggc aatcttgcga tgctgatttc actgattggt acaccgtgga tgccaaaaat
    77101 tgagaacggt attctggtgc tggaagatat taacgagcac cctttccgcg tcgaacgtat
    77161 gctgttgcag ctctatcatg cgggaatttt gccacgtcag aaggcgatta ttctcggtag
    77221 ctttagcggc agcacgccca atgattatga cgcaggttac aacctggagt cagtgtacgc
    77281 gtttttgcgt tcccgcctgt cgattccact cattaccggc ctcgattttg gtcatgaaca
    77341 acgcacggtt acgctgccgt tgggcgcaca tgccattctg aataacaccc gggaaggcac
    77401 tcagttgacg attagcggtc atcctgttct taaaatgtaa aaaaatcacg cttcagggct
    77461 aagtaaaacg ctgtctctgc cgctaatatg gtagggttta tataagagac agcgtaatca
    77521 ggagtaaccg accgttggat gccacaacaa taattagcct tttcatctta ggatccatcc
    77581 ttgtcaccag cagtatatta cttagttcat tttcttcccg tcttggcatt cctattctgg
    77641 ttatttttct ggcgatcggc atgctggcag gagtcgatgg cgtcggcggt atcccgtttg
    77701 ataattaccc cttcgcctac atggtaagta acctggcact ggcgattatt ttgctcgacg
    77761 gcgggatgcg cactcaggcc agttcctttc gtgtggcgtt aggaccggca ctgtcgctgg
    77821 cgacgctggg cgtgcttatc acctctggtt taaccggcat gatggcggcg tggctgttta
    77881 atcttgattt gattgaaggc ttattaatcg gcgctatcgt cggctctacc gatgctgcag
    77941 cggtcttttc tctgctgggt ggtaaggggc ttaacgaacg tgttggctcg acgctggaaa
    78001 ttgaatccgg cagtaatgat ccaatggcgg tctttctgac gattaccctg attgcgatga
    78061 tccagcaaca tgaaagcagt gtcagttgga tgttcgtggt cgatattctg cagcaatttg
    78121 gcctcgggat tgtcatcgga cttggcggtg gttatttact gctgcaaatg attaatcgca
    78181 tcgccctgcc tgccggatta tatccattgc tggcattaag cggcggtatt ttaatttttg
    78241 ctttaaccac ggcgctggaa ggcagcggta ttctggctgt ttatctgtgc ggttttctgc
    78301 tgggtaatcg gccgattcgc aaccgctacg gcatcctgca aaatttcgac ggcctcgcct
    78361 ggctggcgca aatcgccatg ttcctggtgc tggggctatt ggttaaccca agcgatctgc
    78421 tgcctattgc cattccggcg ctcattttgt cggcatggat gatattcttc gcccgtcccc
    78481 tttcggtatt tgctggattg ctccccttcc gcggtttcaa tctgcgtgag cgcgtgttta
    78541 tcagctgggt aggattacgc ggcgcggtgc caatcatcct ggccgtgttc ccgatgatgg
    78601 cggggctgga gaatgcacgt ctgttcttta atgtcgcctt ctttgtggtt ctggtttcac
    78661 tgctcttgca gggaacatca ctctcgtggg cggctaaaaa agccaaagtg gtggttccgc
    78721 cagtgggacg tccggtgtca cgcgttggcc tggatattca tccggaaaat ccgtgggagc
    78781 agtttgttta tcaattgagt gccgataaat ggtgcgtggg cgcggcactg cgcgatttgc
    78841 atatgccaaa agagacgcgt attgcggcac tgtttcgtga taaccagttg cttcatccta
    78901 ccggcagcac ccgactgcgc gaaggcgatg tgttgtgtgt aattggtcgg gaacgcgatc
    78961 ttccggcgct cggtaaactg ttcagccagt cgccgccggt cgcgctggat caacgcttct
    79021 ttggtgactt cattctcgaa gccagcgcca aatatgctga tgttgcgctg atatatggcc
    79081 tggaagacgg gcgagaatat cgtgataagc agcaaacgct gggtgaaatt gtccagcagt
    79141 tgttaggcgc ggcaccggtt gtcggtgacc aggtagagtt tgccgggatg atctggacgg
    79201 tggccgagaa agaagataat gaagtgttga agattggcgt acgggtggct gaagaggaag
    79261 ctgaatctta aatttaatga agcatctatc gccggttgcg atgctttgct gaacgcagcc
    79321 tgagcctgca attgtttgtc gtccataatt taaccttcat ttgatgttga attgaacata
    79381 tcaaaatcag gcaattacac aaatctatgt acaggctcca tcaagggtaa agcgtgattt
    79441 atctgaagtc gagttcgagt caacaatgaa gcgtaagcgt tttatgtaga acgcctgtgc
    79501 ggtgagttca ggttccgggg aaatagtgtc cattctttta atggacacta tcatgggcaa
    79561 tggctctgtc acattaacaa ctacagttgc tgaccagccg gatggatgat tttttcgcaa
    79621 ccagaagacg ctgtctccga tccggcttac aacaagttac actgtcacaa ccgggacgcg
    79681 taacgccagc gcgcacatca actcatagcc caccgttccg gcagcggcgg cgacatcatc
    79741 aattttgatc tccttgcccc acagctcaac cggcgtacca atccccgcct gcgggcaagg
    79801 cgttaaatcg accgctagca tatccatcga gacggtcccc accgtcatgg tgcgcacgcc
    79861 gtccactaaa acaggggtac cggtaggcgc gtggcgcgga taaccgtcgg cgtaccctgc
    79921 ggcgacaatg ccgattcgct gttcatcgcg cgcagtatag cgaccgccgt agcccacacg
    79981 ctcacccgct tttagcgtct ggacaccaat aatctcactg cttagcgtca tcaccggacg
    80041 taatccggta ttggcgatat cacgccactg accggacggc gaagcgccat acaaaataat
    80101 gccaggccga acccagtcaa aatgcgcttc cgggtgccac agagtcgccg ccgaattgga
    80161 caacgaacgc cgacactcca gcccttccgc cgcctgctca atacgcgcca tcgcgctgga
    80221 aattccatca ggatgttccg cttcggcaaa atgcgacatc agggtcatct cgccaacatt
    80281 cgccattgcc cgcaactgct gccagacggt aagcacgcga tcgggctgga agcccaaccg
    80341 attcatccca ctgttcactt taagataaat atccaacggt gcttttagcc gcgcattttg
    80401 cagtgctttg agctgccagt tgctgtgtac gcaggtggtc aggcggtgct ggtcataaat
    80461 ctccagatcc tgagcatgga aaaatccttc cagcatcagg atcggcccct tccagccgcg
    80521 ctcacgtaac gttattgcct cttccaggtt aagtaatgca aagccatcgg tggccccgag
    80581 cgcgctccag atacgctcaa taccatgccc gtaagcgttc gcttttacca ccgaccagac
    80641 gcgcgcgtgc ggcgcggcct ggcggacaat ggacagattc tgttttaatg cctgcagatc
    80701 gaggctggcc tgtatcggac gggtcatctc gtttccttag ctgtgtgcgc catgtaaatg
    80761 gcccggacgt gatggcgtaa atccacggct gtagcgcgct acgcttagat cctcatatgg
    80821 gatcgctggc gtgcgaccag agagcagatc gcttaacaac tgaccggaac cgcaagccat
    80881 cgtccagccg agcgtgccgt gaccggtatt cagccacaga tttttaaagc gtgtacgccc
    80941 gacaaccggc gtaccgtctg gcgtcatcgg gcgcaaaccc gtccagaaag tcgcctgctc
    81001 gacatgaccg ccgcgtggat agagatcgcg aaccaccatt tccagcgttt cacgacgcgg
    81061 ttgcaacagc tcggtgttaa aaccaacaat ttccgccatt ccgccaacac gaatgcggtt
    81121 atcgaaacgg gtaatggcga ttttgtaggt ttcatcaaga atggtggata ctggcgcacc
    81181 atcttcctgc gcaattggaa tagtcagcga gtagcctttc agcggataca ccggaatatc
    81241 aacaatgcct ttgagcatcg ccgtcgagta agaaccaaac gccatcacat acgcatcggc
    81301 cttaatcact tcatcgccac acttcacgcc gtagatttgc tcgccgtcgc aaagcagttg
    81361 gtcaacgggc gtattaaagc ggaatttaac ccccgcctgc tctgccatcc gcgccagatt
    81421 ctgggtaaac agctggcagt ctccggtttc atcattgggc agttgcagac cacccgtcag
    81481 tttgtgcgct acttctgcca gcgcgggctc cacttccgcc aagcggctgg attccagcag
    81541 ctgatacggt acgccggcat cttccagcac ggcgatatcg cgggtcgcat tttcatactg
    81601 ttgttcggta cggaacagtt gcagcgtccc accctgacgc ccttcatact gaatattggt
    81661 ttcggcgcgc aatgctttca ggcaatcacg gctgtattcc gccagacgca ccatccgccc
    81721 tttgttttcc atgtagtggc tggtgtcgca gttacgtaac atttgccaca tccatttcaa
    81781 ctggaactgc gtaccgtcga gacgaaccgc cagcggcgca tggcgctgga acatccattt
    81841 aatcgctttt aaaggcacac ctggtgccgc ccacggtgca gcatatccgg gggagatttg
    81901 cccggcattc gcggcgctgg tttccagggc ggcccccggc tcccgatcaa tgacggtgac
    81961 ctcatgtcct gcctgattta agtaccaggc gctggcaacg cctaccacac cacttcccag
    82021 tatgacaact cgcatagcca ctccgataac ggtagataca gtaaaagaat aataatctaa
    82081 ttacatcttg ataactcagt tgaaaatatt attcaacata cggctttttt atggtgagct
    82141 ggctcacatc tccctgactc tgcggcaata tggcgtcttt agtatctcct gctgaacgcc
    82201 ctttttatcc agcataaaat tccgttcaga agcggattag tggcactctg ataataagaa
    82261 atcgcaaaga aaaaaattct ccggcgacgc ggtttttaac aggtgttcta tgcttgaaat
    82321 gaggtgctcc acacagagag cgccgacaac aatgaggtgc gcgtatggcg acgatcgatt
    82381 ctatgaataa ggacaccaca cgtttgagcg atggacccga ctggacgttc gacctgctgg
    82441 atgtttatct ggcagagata gaccgggtgg cgaaactcta ccggctggat acctacccgc
    82501 accagattga agtgataacc tcagaacaga tgatggatgc ctactccagc gtcggcatgc
    82561 caattaacta tccgcactgg tcattcggta aaaagtttat cgagactgaa cggctgtata
    82621 agcacggtca gcaaggactg gcctatgaaa tcgtcattaa ctctaacccg tgtatcgctt
    82681 acctgatgga agagaacacc attaccatgc aggcgctggt gatggcacac gcctgctatg
    82741 ggcataactc tttctttaaa aacaattact tattccgcag ttggaccgac gccagttcga
    82801 ttgtcgatta tctgattttt gcccgtaaat atattaccga gtgcgaagaa cgttatggcg
    82861 ttgatgaagt agaacggctt ctggactcgt gccacgcgct gatgaactac ggcgtggacc
    82921 ggtacaaacg cccgcaaaaa atctcgctgc aagaagagaa agcccggcag aaaagtcgcg
    82981 aagagtatct acaaagtcag gtcaatatgc tctggcgtac cctgccgaag cgcgaggaag
    83041 agaaaacggt tgctgaagcg cgccgctatc cgtccgaacc acaagaaaac ctgctctatt
    83101 ttatggagaa aaatgcgcca ctgctggaat catggcagcg tgaaatcctg cgtattgtgc
    83161 gtaaggtgag ccagtatttt tatccgcaaa aacagactca ggtgatgaac gaaggctggg
    83221 cgaccttctg gcactatacc atccttaacc atctgtatga tgaagggaaa gtaacggaac
    83281 gttttatgct ggagtttttg cacagccaca ccaatgtggt cttccagccc ccctataaca
    83341 gcccgtggta cagcggcatc aacccgtatg ccctcgggtt cgccatgttc caggatatta
    83401 aacggatttg tcagtcgcca acggaagaag acaaatactg gttcccggat atcgccggtt
    83461 ccgactggct ggaaacgctg catttcgcga tgcgtgattt caaagatgag agttttatca
    83521 gccagtttct gtcaccgaaa gtgatgcgtg atttccgctt cttcaccgtg ctggatgatg
    83581 atcggcataa ttatctggag atttccgcta ttcataatga agaaggttat cgggagatcc
    83641 gtaaccggtt atcgtcgcaa tataacttaa gtaatctgga gccgaatatt cagatctgga
    83701 acgtggattt gcgcggcgac cgttcgctga cgctgcgtta cattccacat aatcgcgcac
    83761 cgctggatcg ggggcgcaaa gaagtgctga agcatgtgca tcgcctgtgg ggatttgatg
    83821 tgatgctcga acagcaaaac gaagacggca gcatcgagtt gctggaacgt tgcccgccaa
    83881 gaatgggaaa tctgtaaaaa caacaaaaaa cccctcgttt gaggggtttg ctctttaaac
    83941 ggaagggatt atcgcccctg aatggctaaa tcacccggca gatttttctg catccggtgc
    84001 caaatctcgc cactctcatg cccatagcga cgcactgttt cgtacacctg atcgtgcgcg
    84061 ccttcactgc acaacgccga cagtttgtgg tagaagccca gcgccagact gcgcgcttcc
    84121 ggattggcga aatagtgacg accaatgcgc gtatacagcc ctttcatccc gttaaggatc
    84181 agaccgtaaa tcgggttgcc ggaagcaaac gccaggccgc ggaatatgtt gtaatccagc
    84241 tcggcaaagg catcggcgtg atcggccact tcattagcgg tagccagcac ttcctgcgct
    84301 ttatcgggat gctgacgaaa cgcggtgcga ataaaaatag tggaaatatt ggtacgcacc
    84361 gacagcaaat tatcaataag ctgcggcaca ctttcgtgat ccagtcgcgc cagtgtttca
    84421 aggatattta aaccagaggt ttcccagaaa ttattcacct tcgtcggctt gccatgttga
    84481 atggtcaacc agccatctcg tgccagacgc tgtaacactt cacgtaacgt agtacgcgtt
    84541 acgccaatta attctgaaag ttcacgttct gcgggcaaaa tagtcccggg agggaagcgg
    84601 ttattccaga tactttcaat aatgtactct tccgcgaaac ccgccgggct ttgcgcctta
    84661 atgaccatag tgagatttcc ataacacagc gaaacaaagt tggactcatc ataccagagg
    84721 gctaactacg ctgatagcag acaaaaagaa gaaaaaggga tcaaggcttc attttccgca
    84781 tctctttccc cttgactatc aagacttgcc tcactcctgc ctgaccgtta agctttgcgt
    84841 ttaaacataa aaacatcatt gattatttgt aaggtaaggg aaaccatcat ggagatctcc
    84901 tggggccgcg cgctatggcg caattttttg ggccagtccc ccgactggta caaactcgcc
    84961 ctcatcattt tcttaatcgt aaacccgtta attttcctca tcagcccttt cgtcgcgggc
    85021 tggttgctgg tagcggaatt tattttcact ctggcgatgg ccctgaaatg ctacccgcta
    85081 ctccccggtg gtctgttggc tatcgaagcg gtattcatcg gcatgaccag cgcggaacac
    85141 atccgtgaag aggtggcggc aaatcttgaa gtcttgctgt tattgatgtt tatggtggcg
    85201 ggtatctatt ttatgaaaca gctgttgctg ttcatattta cccgtttgct gttaagcatt
    85261 cgctccaaaa tgctgctgtc gctctctttt tgcgtggcgg cggcgttcct ctccgcgttc
    85321 ctcgatgcct taaccgtcgt ggcggtggtg atcagcgttg cagtcggttt ttatggtatt
    85381 tatcaccgcg tagcctcttc ccgtactgaa gataccgacc tgcaagacga tagtcatatc
    85441 gacaagcatt acaaagtggt tctggaacag ttccgtggct ttctgcgtag cctgatgatg
    85501 catgccggtg tcggcactgc attaggcggc gtgatgacca tggtgggcga accacagaac
    85561 ctgatcatcg ctaaagcggc tggctggcat tttggcgatt tcttcctgcg catgtcgccg
    85621 gtgaccgttc cggttctgat ttgtggcctg ttaacctgcc tgctggtaga gaagctgcgt
    85681 tggtttggct acggtgaaac gctgccggag aaagtccgcg aagtgttgca acagtttgac
    85741 gatcaaagcc gccaccagcg tacccgtcag gataaaatcc gtctgattgt ccaggcgatt
    85801 attggcgtct ggctggtgac tgcgctggcg ttgcatctgg cggaagttgg cttgattggt
    85861 ttgtcagtca ttattctggc gacatcattg accggtgtca ccgatgaaca tgctatcggt
    85921 aaagccttca ccgaatctct gccattcacc gcactgttga cggtgttttt ctcggtagtc
    85981 gcggtgatta tcgaccaaca actgttttcg ccaattattc agtttgtgtt gcaggcatcg
    86041 gaacatgctc agctgtcgct gttctatatt ttcaacggtc tgctgtcatc catttcggat
    86101 aacgtcttcg tggggacgat ttatatcaac gaagcgaaag cggcaatgga aagtggcgct
    86161 atcacgttga agcaatacga gctgctggcg gtcgccatta ataccggtac caatctgccc
    86221 tccgtcgcta cgccgaacgg tcaggctgcg ttcctgttcc tgctgacctc tgcactcgcg
    86281 ccattgattc gcctctctta tggccgcatg gtgtggatgg ccctgcctta caccctcgta
    86341 ctgacactcg tcggcttgct ctgcgtcgag tttacgcttg cccctgtaac cgaatggttt
    86401 atgcaaatgg gctggatagc aacgctttga taacaactta ccgggcatat aaatgcccgg
    86461 tttgcctttt cgcccgataa ttgtccaatt gcttcctatt tttgctgcct cctggtggcg
    86521 cagcgaatga attggtttaa actgcgcact ctatgcatat tgcagggaaa tgattatgtt
    86581 gcgatttttg aaccaatgtt cacaaggccg gggcgcgtgg ctgttgatgg cgtttactgc
    86641 tctggcactg gaactgacgg cgctgtggtt ccagcatgtg atgttactga aaccttgcgt
    86701 gctctgtatt tatgaacgtt gtgcgttatt cggcgttctg ggtgctgcgc tgattggcgc
    86761 gatcgccccg aaaactccgc tgcgttatgt agcgatggtt atctggttgt atagtgcgtt
    86821 ccgcggtgtg cagttaactt acgagcacac catgcttcag ctctatcctt cgccgtttgc
    86881 cacctgtgat tttatggttc gtttcccgga atggctgccg ctggataaat gggtgccgca
    86941 agtgtttgtc gcctctggcg attgtgccga gcgtcagtgg gattttttag gtatggaaat
    87001 gccgcagtgg ctgctcggta tttttatcgc ttacctgatt gtcgcggtgc tggtggtgat
    87061 ttcccagccg tttaaagcga aaaaacgtga cctgttcggt cgctaatccc ttcagcgctc
    87121 ctgcgggagc gcttttttcc tgccgctata tttatttgac ccgcagtaaa tcagaacttc
    87181 gcgttgtata acgtggtgaa agcatggcac gcttcatctg ccattgttgc tggatcccct
    87241 gcccggcaaa atagagtgtt cctctgccct ctttagcatt cagtgtatcc attaccgtca
    87301 tcaattgctc actcccgggg cgcggtgcgt tgtcatcgaa taaattgagc tgcgcgactc
    87361 cctgactgaa gaaatccccc agcatcacgc ccgctttttg gtagcgatgg cccgcttgcc
    87421 agatggcatc cagagatcgc gtagcagcgt taatgatatc cctgctgtcc tgagtgggcg
    87481 tcagcagttt taccgacgcg ctattgccgt aataaggttc attgagcgca aatggtgacg
    87541 tcttaataaa tgtggagata aaccgacaat attgatgctc gctgcgaagt ttttccgccg
    87601 cccgggcagc gtaactacaa atggcctgcc gcatcgatgg ataatccgtg atgcgttcac
    87661 caaacgagcg ggaacagata atttcctgct tcgtcggtgc aaactcttcc agttgcaaac
    87721 agggttcgcc gcgcagttca cgcaccgttc tttcgagcac gacattaaaa tgtttacgga
    87781 taaaccggat atctgtatcc gccaaatcga gaacggtttt gatccccatc gcgtccagtt
    87841 ttttgctgat ccgccgtcca atcccccaga cgtcatccac ggggagagca gacattaatt
    87901 tacgctggcg ttccagattt gataaatcca ccaccccacc cgtctgccgc tgccattttt
    87961 ttgccgcatg attggcaagc ttagccaacg ttttggtctg ggcgatcccc acaccaacag
    88021 taagatgggt acgttgtagc accgttgcgc gaatttctct gccaaaatca gtcagatcgc
    88081 gacaattacg cacacctgtc agatcgcaga atgcctcatc aatactgtaa atctcgacgc
    88141 ggggcgatag ttcttccagc gtcgacatca cccgattgct catgtctgcg taaagctcat
    88201 aattgctgct aaagcaaacc acgccacagc gacgaaacag atctttttgt ttgaaccagg
    88261 gatcgcccat tttaacaccc agcgcctttg cctcagcgtt tcgggcgata acgcaaccgt
    88321 cattattcga tagcacaacc accggtttac cccataaatc agggcgaaac accgtctcac
    88381 agctggcata aaacgcgttt acatcacaga gagcaaacat cagcgcatcg ccttaacgac
    88441 gtggatcacc acaccaaaga catccagcgt atcttcgcta ctgatggtaa tgggcgagta
    88501 cgcactgttc atgggaataa gctgtaccgt cgggcgtagt tgcaattttt tcaccgtaaa
    88561 ctcgccgtca acagcagcga tgacaatatc accatggctg gcggtaatag cgctatcgac
    88621 aatcagtaaa tcaccgtcac taattccacc atcaatcata gaatcaccac ttgctttgac
    88681 gaagtaagtc gcgctgggat gctggatcaa cagttgattc agatcgatgc gctgttcaac
    88741 gtaatctgct gccggtgaag gaaagccaca ctgaacaaga tcgctaaata gcggaaaagt
    88801 cacaatttcg cggagatccg caggcttgat aaacaacata ataatctgcc tgaagttata
    88861 ctgtttttat atacagtagt ctgttcttgc cagcagatca atactgattc aggctatcaa
    88921 tatttgtcgc tgcatagact gctgattttt cgttctctta tcctgtgctc acgtggcctt
    88981 ctggcgacga cgctcatcca gcagaaatga aaaatatcac ccggcaaaaa aatagaatag
    89041 aagcattcgc cataatgaca tt
//
LOCUS       DAZGAN020000004        87069 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGAN020000004 DAZGAN020000000
VERSION     DAZGAN020000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 87069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 87069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.004"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..309)
                     /locus_tag="ACHKYO_RS01555"
                     /old_locus_tag="pgaptmp_000311"
     CDS             complement(<1..309)
                     /locus_tag="ACHKYO_RS01555"
                     /old_locus_tag="pgaptmp_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU4427211.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     I"
     gene            complement(380..2494)
                     /gene="fusA"
                     /locus_tag="ACHKYO_RS01560"
                     /old_locus_tag="pgaptmp_000312"
     CDS             complement(380..2494)
                     /gene="fusA"
                     /locus_tag="ACHKYO_RS01560"
                     /old_locus_tag="pgaptmp_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGU4427212.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2591..3061)
                     /gene="rpsG"
                     /locus_tag="ACHKYO_RS01565"
                     /old_locus_tag="pgaptmp_000313"
     CDS             complement(2591..3061)
                     /gene="rpsG"
                     /locus_tag="ACHKYO_RS01565"
                     /old_locus_tag="pgaptmp_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGU4427213.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3158..3532)
                     /gene="rpsL"
                     /locus_tag="ACHKYO_RS01570"
                     /old_locus_tag="pgaptmp_000314"
     CDS             complement(3158..3532)
                     /gene="rpsL"
                     /locus_tag="ACHKYO_RS01570"
                     /old_locus_tag="pgaptmp_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGU4427214.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3658..3945)
                     /gene="tusB"
                     /locus_tag="ACHKYO_RS01575"
                     /old_locus_tag="pgaptmp_000315"
     CDS             complement(3658..3945)
                     /gene="tusB"
                     /locus_tag="ACHKYO_RS01575"
                     /old_locus_tag="pgaptmp_000315"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HGU4427215.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3953..4312)
                     /gene="tusC"
                     /locus_tag="ACHKYO_RS01580"
                     /old_locus_tag="pgaptmp_000316"
     CDS             complement(3953..4312)
                     /gene="tusC"
                     /locus_tag="ACHKYO_RS01580"
                     /old_locus_tag="pgaptmp_000316"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HGU4427216.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4312..4698)
                     /gene="tusD"
                     /locus_tag="ACHKYO_RS01585"
                     /old_locus_tag="pgaptmp_000317"
     CDS             complement(4312..4698)
                     /gene="tusD"
                     /locus_tag="ACHKYO_RS01585"
                     /old_locus_tag="pgaptmp_000317"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HGU4427217.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4698..5420)
                     /gene="yheO"
                     /locus_tag="ACHKYO_RS01590"
                     /old_locus_tag="pgaptmp_000318"
     CDS             complement(4698..5420)
                     /gene="yheO"
                     /locus_tag="ACHKYO_RS01590"
                     /old_locus_tag="pgaptmp_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4427218.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5587..6399)
                     /gene="fkpA"
                     /locus_tag="ACHKYO_RS01595"
                     /old_locus_tag="pgaptmp_000319"
     CDS             complement(5587..6399)
                     /gene="fkpA"
                     /locus_tag="ACHKYO_RS01595"
                     /old_locus_tag="pgaptmp_000319"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4427219.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6620..6838
                     /gene="slyX"
                     /locus_tag="ACHKYO_RS01600"
                     /old_locus_tag="pgaptmp_000320"
     CDS             6620..6838
                     /gene="slyX"
                     /locus_tag="ACHKYO_RS01600"
                     /old_locus_tag="pgaptmp_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HGU4427220.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMEKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6887..7477)
                     /gene="slyD"
                     /locus_tag="ACHKYO_RS01605"
                     /old_locus_tag="pgaptmp_000321"
     CDS             complement(6887..7477)
                     /gene="slyD"
                     /locus_tag="ACHKYO_RS01605"
                     /old_locus_tag="pgaptmp_000321"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU4427221.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7572..7772)
                     /gene="yheV"
                     /locus_tag="ACHKYO_RS01610"
                     /old_locus_tag="pgaptmp_000322"
     CDS             complement(7572..7772)
                     /gene="yheV"
                     /locus_tag="ACHKYO_RS01610"
                     /old_locus_tag="pgaptmp_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HGU4427222.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7782..9587)
                     /gene="kefB"
                     /locus_tag="ACHKYO_RS01615"
                     /old_locus_tag="pgaptmp_000323"
     CDS             complement(7782..9587)
                     /gene="kefB"
                     /locus_tag="ACHKYO_RS01615"
                     /old_locus_tag="pgaptmp_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HGU4427223.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9587..10138)
                     /gene="kefG"
                     /locus_tag="ACHKYO_RS01620"
                     /old_locus_tag="pgaptmp_000324"
     CDS             complement(9587..10138)
                     /gene="kefG"
                     /locus_tag="ACHKYO_RS01620"
                     /old_locus_tag="pgaptmp_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HGU4427224.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10269..12182
                     /gene="yheS"
                     /locus_tag="ACHKYO_RS01625"
                     /old_locus_tag="pgaptmp_000325"
     CDS             10269..12182
                     /gene="yheS"
                     /locus_tag="ACHKYO_RS01625"
                     /old_locus_tag="pgaptmp_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4427225.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDDAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12182..13204
                     /gene="yheT"
                     /locus_tag="ACHKYO_RS01630"
                     /old_locus_tag="pgaptmp_000326"
     CDS             12182..13204
                     /gene="yheT"
                     /locus_tag="ACHKYO_RS01630"
                     /old_locus_tag="pgaptmp_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU4427226.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13198..13416
                     /gene="yheU"
                     /locus_tag="ACHKYO_RS01635"
                     /old_locus_tag="pgaptmp_000327"
     CDS             13198..13416
                     /gene="yheU"
                     /locus_tag="ACHKYO_RS01635"
                     /old_locus_tag="pgaptmp_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HGU4427227.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13470..14339
                     /gene="prkB"
                     /locus_tag="ACHKYO_RS01640"
                     /old_locus_tag="pgaptmp_000328"
     CDS             13470..14339
                     /gene="prkB"
                     /locus_tag="ACHKYO_RS01640"
                     /old_locus_tag="pgaptmp_000328"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HGU4427228.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14394..14798)
                     /gene="yhfA"
                     /locus_tag="ACHKYO_RS01645"
                     /old_locus_tag="pgaptmp_000329"
     CDS             complement(14394..14798)
                     /gene="yhfA"
                     /locus_tag="ACHKYO_RS01645"
                     /old_locus_tag="pgaptmp_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HGU4427229.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAV
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15100..15732
                     /gene="crp"
                     /locus_tag="ACHKYO_RS01650"
                     /old_locus_tag="pgaptmp_000330"
     CDS             15100..15732
                     /gene="crp"
                     /locus_tag="ACHKYO_RS01650"
                     /old_locus_tag="pgaptmp_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HGU4427230.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15783..17873
                     /gene="yhfK"
                     /locus_tag="ACHKYO_RS01655"
                     /old_locus_tag="pgaptmp_000331"
     CDS             15783..17873
                     /gene="yhfK"
                     /locus_tag="ACHKYO_RS01655"
                     /old_locus_tag="pgaptmp_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HGU4427231.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLANY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17940..19160)
                     /gene="argD"
                     /locus_tag="ACHKYO_RS01660"
                     /old_locus_tag="pgaptmp_000332"
                     /gene_synonym="dapC"
     CDS             complement(17940..19160)
                     /gene="argD"
                     /locus_tag="ACHKYO_RS01660"
                     /old_locus_tag="pgaptmp_000332"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HGU4427232.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLSCAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19246..19809)
                     /gene="pabA"
                     /locus_tag="ACHKYO_RS01665"
                     /old_locus_tag="pgaptmp_000333"
     CDS             complement(19246..19809)
                     /gene="pabA"
                     /locus_tag="ACHKYO_RS01665"
                     /old_locus_tag="pgaptmp_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HGU4427233.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVELGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19841..20443)
                     /gene="fic"
                     /locus_tag="ACHKYO_RS01670"
                     /old_locus_tag="pgaptmp_000334"
     CDS             complement(19841..20443)
                     /gene="fic"
                     /locus_tag="ACHKYO_RS01670"
                     /old_locus_tag="pgaptmp_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HGU4427234.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYKL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20433..20600)
                     /gene="yhfG"
                     /locus_tag="ACHKYO_RS01675"
                     /old_locus_tag="pgaptmp_000335"
     CDS             complement(20433..20600)
                     /gene="yhfG"
                     /locus_tag="ACHKYO_RS01675"
                     /old_locus_tag="pgaptmp_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HGU4427235.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20705..21277)
                     /gene="ppiA"
                     /locus_tag="ACHKYO_RS01680"
                     /old_locus_tag="pgaptmp_000336"
                     /gene_synonym="rotA"
     CDS             complement(20705..21277)
                     /gene="ppiA"
                     /locus_tag="ACHKYO_RS01680"
                     /old_locus_tag="pgaptmp_000336"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HGU4427236.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21548..22729
                     /gene="tsgA"
                     /locus_tag="ACHKYO_RS01685"
                     /old_locus_tag="pgaptmp_000337"
     CDS             21548..22729
                     /gene="tsgA"
                     /locus_tag="ACHKYO_RS01685"
                     /old_locus_tag="pgaptmp_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HGU4427237.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAAVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22991..25534
                     /gene="nirB"
                     /locus_tag="ACHKYO_RS01690"
                     /old_locus_tag="pgaptmp_000338"
     CDS             22991..25534
                     /gene="nirB"
                     /locus_tag="ACHKYO_RS01690"
                     /old_locus_tag="pgaptmp_000338"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HGU4427238.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25531..25857
                     /gene="nirD"
                     /locus_tag="ACHKYO_RS01695"
                     /old_locus_tag="pgaptmp_000339"
     CDS             25531..25857
                     /gene="nirD"
                     /locus_tag="ACHKYO_RS01695"
                     /old_locus_tag="pgaptmp_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HGU4427239.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25984..26790
                     /gene="nirC"
                     /locus_tag="ACHKYO_RS01700"
                     /old_locus_tag="pgaptmp_000340"
     CDS             25984..26790
                     /gene="nirC"
                     /locus_tag="ACHKYO_RS01700"
                     /old_locus_tag="pgaptmp_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HGU4427240.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26809..28182
                     /gene="cysG"
                     /locus_tag="ACHKYO_RS01705"
                     /old_locus_tag="pgaptmp_000341"
     CDS             26809..28182
                     /gene="cysG"
                     /locus_tag="ACHKYO_RS01705"
                     /old_locus_tag="pgaptmp_000341"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HGU4427241.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28429..28596
                     /gene="yhfL"
                     /locus_tag="ACHKYO_RS01710"
                     /old_locus_tag="pgaptmp_000342"
     CDS             28429..28596
                     /gene="yhfL"
                     /locus_tag="ACHKYO_RS01710"
                     /old_locus_tag="pgaptmp_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HGU4427242.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28892..30229
                     /gene="frlA"
                     /locus_tag="ACHKYO_RS01715"
                     /old_locus_tag="pgaptmp_000343"
     CDS             28892..30229
                     /gene="frlA"
                     /locus_tag="ACHKYO_RS01715"
                     /old_locus_tag="pgaptmp_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HGU4427243.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLPGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30250..31272
                     /gene="frlB"
                     /locus_tag="ACHKYO_RS01720"
                     /old_locus_tag="pgaptmp_000344"
     CDS             30250..31272
                     /gene="frlB"
                     /locus_tag="ACHKYO_RS01720"
                     /old_locus_tag="pgaptmp_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HGU4427244.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31322..32203
                     /gene="frlC"
                     /locus_tag="ACHKYO_RS01725"
                     /old_locus_tag="pgaptmp_000345"
     CDS             31322..32203
                     /gene="frlC"
                     /locus_tag="ACHKYO_RS01725"
                     /old_locus_tag="pgaptmp_000345"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HGU4427245.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYDGYCTVELVTMYMNEPRLYACQALERFRALLPEDER
                     WKPWRQSAITASISTRN"
     gene            32149..32934
                     /gene="frlD"
                     /locus_tag="ACHKYO_RS01730"
                     /old_locus_tag="pgaptmp_000346"
     CDS             32149..32934
                     /gene="frlD"
                     /locus_tag="ACHKYO_RS01730"
                     /old_locus_tag="pgaptmp_000346"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HGU4427246.1"
                     /translation="METLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33034..33765
                     /gene="frlR"
                     /locus_tag="ACHKYO_RS01735"
                     /old_locus_tag="pgaptmp_000347"
                     /gene_synonym="yhfR"
     CDS             33034..33765
                     /gene="frlR"
                     /locus_tag="ACHKYO_RS01735"
                     /old_locus_tag="pgaptmp_000347"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HGU4427247.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33917..35002)
                     /gene="yhfS"
                     /locus_tag="ACHKYO_RS01740"
                     /old_locus_tag="pgaptmp_000348"
     CDS             complement(33917..35002)
                     /gene="yhfS"
                     /locus_tag="ACHKYO_RS01740"
                     /old_locus_tag="pgaptmp_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGU4427248.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35014..36318)
                     /gene="yhfT"
                     /locus_tag="ACHKYO_RS01745"
                     /old_locus_tag="pgaptmp_000349"
     CDS             complement(35014..36318)
                     /gene="yhfT"
                     /locus_tag="ACHKYO_RS01745"
                     /old_locus_tag="pgaptmp_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HGU4427249.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36330..36683)
                     /gene="yhfU"
                     /locus_tag="ACHKYO_RS01750"
                     /old_locus_tag="pgaptmp_000350"
     CDS             complement(36330..36683)
                     /gene="yhfU"
                     /locus_tag="ACHKYO_RS01750"
                     /old_locus_tag="pgaptmp_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HGU4427250.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36694..37572)
                     /gene="php"
                     /locus_tag="ACHKYO_RS01755"
                     /old_locus_tag="pgaptmp_000351"
     CDS             complement(36694..37572)
                     /gene="php"
                     /locus_tag="ACHKYO_RS01755"
                     /old_locus_tag="pgaptmp_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HGU4427251.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37569..38795)
                     /gene="yhfW"
                     /locus_tag="ACHKYO_RS01760"
                     /old_locus_tag="pgaptmp_000352"
     CDS             complement(37569..38795)
                     /gene="yhfW"
                     /locus_tag="ACHKYO_RS01760"
                     /old_locus_tag="pgaptmp_000352"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGU4427252.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLLVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38795..39958)
                     /gene="yhfX"
                     /locus_tag="ACHKYO_RS01765"
                     /old_locus_tag="pgaptmp_000353"
     CDS             complement(38795..39958)
                     /gene="yhfX"
                     /locus_tag="ACHKYO_RS01765"
                     /old_locus_tag="pgaptmp_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HGU4427253.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(40042..40404)
                     /gene="yhfY"
                     /locus_tag="ACHKYO_RS01770"
                     /old_locus_tag="pgaptmp_000354"
     CDS             complement(40042..40404)
                     /gene="yhfY"
                     /locus_tag="ACHKYO_RS01770"
                     /old_locus_tag="pgaptmp_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGU4427254.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40421..41326)
                     /gene="yhfZ"
                     /locus_tag="ACHKYO_RS01775"
                     /old_locus_tag="pgaptmp_000355"
     CDS             complement(40421..41326)
                     /gene="yhfZ"
                     /locus_tag="ACHKYO_RS01775"
                     /old_locus_tag="pgaptmp_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HGU4427255.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41595..42599)
                     /gene="trpS"
                     /locus_tag="ACHKYO_RS01780"
                     /old_locus_tag="pgaptmp_000356"
     CDS             complement(41595..42599)
                     /gene="trpS"
                     /locus_tag="ACHKYO_RS01780"
                     /old_locus_tag="pgaptmp_000356"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGU4427256.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42592..43350)
                     /gene="gph"
                     /locus_tag="ACHKYO_RS01785"
                     /old_locus_tag="pgaptmp_000357"
     CDS             complement(42592..43350)
                     /gene="gph"
                     /locus_tag="ACHKYO_RS01785"
                     /old_locus_tag="pgaptmp_000357"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HGU4427257.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43343..44020)
                     /gene="rpe"
                     /locus_tag="ACHKYO_RS01790"
                     /old_locus_tag="pgaptmp_000358"
     CDS             complement(43343..44020)
                     /gene="rpe"
                     /locus_tag="ACHKYO_RS01790"
                     /old_locus_tag="pgaptmp_000358"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGU4427258.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44038..44874)
                     /gene="dam"
                     /locus_tag="ACHKYO_RS01795"
                     /old_locus_tag="pgaptmp_000359"
     CDS             complement(44038..44874)
                     /gene="dam"
                     /locus_tag="ACHKYO_RS01795"
                     /old_locus_tag="pgaptmp_000359"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU4427259.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44981..46267)
                     /gene="damX"
                     /locus_tag="ACHKYO_RS01800"
                     /old_locus_tag="pgaptmp_000360"
     CDS             complement(44981..46267)
                     /gene="damX"
                     /locus_tag="ACHKYO_RS01800"
                     /old_locus_tag="pgaptmp_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HGU4427260.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46359..47447)
                     /gene="aroB"
                     /locus_tag="ACHKYO_RS01805"
                     /old_locus_tag="pgaptmp_000361"
     CDS             complement(46359..47447)
                     /gene="aroB"
                     /locus_tag="ACHKYO_RS01805"
                     /old_locus_tag="pgaptmp_000361"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGU4427261.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47504..48025)
                     /gene="aroK"
                     /locus_tag="ACHKYO_RS01810"
                     /old_locus_tag="pgaptmp_000362"
     CDS             complement(47504..48025)
                     /gene="aroK"
                     /locus_tag="ACHKYO_RS01810"
                     /old_locus_tag="pgaptmp_000362"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HGU4427262.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48426..49664)
                     /gene="hofQ"
                     /locus_tag="ACHKYO_RS01815"
                     /old_locus_tag="pgaptmp_000363"
     CDS             complement(48426..49664)
                     /gene="hofQ"
                     /locus_tag="ACHKYO_RS01815"
                     /old_locus_tag="pgaptmp_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HGU4427263.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49576..49980)
                     /gene="hofP"
                     /locus_tag="ACHKYO_RS01820"
                     /old_locus_tag="pgaptmp_000364"
     CDS             complement(49576..49980)
                     /gene="hofP"
                     /locus_tag="ACHKYO_RS01820"
                     /old_locus_tag="pgaptmp_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HGU4427264.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49970..50410)
                     /gene="hofO"
                     /locus_tag="ACHKYO_RS01825"
                     /old_locus_tag="pgaptmp_000365"
     CDS             complement(49970..50410)
                     /gene="hofO"
                     /locus_tag="ACHKYO_RS01825"
                     /old_locus_tag="pgaptmp_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HGU4427265.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50394..50933)
                     /gene="hofN"
                     /locus_tag="ACHKYO_RS01830"
                     /old_locus_tag="pgaptmp_000366"
     CDS             complement(50394..50933)
                     /gene="hofN"
                     /locus_tag="ACHKYO_RS01830"
                     /old_locus_tag="pgaptmp_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HGU4427266.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50933..51712)
                     /gene="hofM"
                     /locus_tag="ACHKYO_RS01835"
                     /old_locus_tag="pgaptmp_000367"
     CDS             complement(50933..51712)
                     /gene="hofM"
                     /locus_tag="ACHKYO_RS01835"
                     /old_locus_tag="pgaptmp_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HGU4427267.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPSGGDFAIALGLALGKAY"
     gene            51832..54384
                     /gene="mrcA"
                     /locus_tag="ACHKYO_RS01840"
                     /old_locus_tag="pgaptmp_000368"
     CDS             51832..54384
                     /gene="mrcA"
                     /locus_tag="ACHKYO_RS01840"
                     /old_locus_tag="pgaptmp_000368"
                     /EC_number="2.4.99.28"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HGU4427268.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDESL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54549..55109)
                     /gene="nudE"
                     /locus_tag="ACHKYO_RS01845"
                     /old_locus_tag="pgaptmp_000369"
     CDS             complement(54549..55109)
                     /gene="nudE"
                     /locus_tag="ACHKYO_RS01845"
                     /old_locus_tag="pgaptmp_000369"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HGU4427269.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55429..57564
                     /gene="igaA"
                     /locus_tag="ACHKYO_RS01850"
                     /old_locus_tag="pgaptmp_000370"
     CDS             55429..57564
                     /gene="igaA"
                     /locus_tag="ACHKYO_RS01850"
                     /old_locus_tag="pgaptmp_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HGU4427270.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVHQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVFLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57629..58297
                     /gene="yrfG"
                     /locus_tag="ACHKYO_RS01855"
                     /old_locus_tag="pgaptmp_000371"
     CDS             57629..58297
                     /gene="yrfG"
                     /locus_tag="ACHKYO_RS01855"
                     /old_locus_tag="pgaptmp_000371"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HGU4427271.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58308..58709
                     /gene="hslR"
                     /locus_tag="ACHKYO_RS01860"
                     /old_locus_tag="pgaptmp_000372"
     CDS             58308..58709
                     /gene="hslR"
                     /locus_tag="ACHKYO_RS01860"
                     /old_locus_tag="pgaptmp_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HGU4427272.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58734..59612
                     /gene="hslO"
                     /locus_tag="ACHKYO_RS01865"
                     /old_locus_tag="pgaptmp_000373"
     CDS             58734..59612
                     /gene="hslO"
                     /locus_tag="ACHKYO_RS01865"
                     /old_locus_tag="pgaptmp_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGU4427273.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59747..61471)
                     /gene="yhgE"
                     /locus_tag="ACHKYO_RS01870"
                     /old_locus_tag="pgaptmp_000374"
     CDS             complement(59747..61471)
                     /gene="yhgE"
                     /locus_tag="ACHKYO_RS01870"
                     /old_locus_tag="pgaptmp_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HGU4427274.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRICNGSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61850..63472
                     /gene="pckA"
                     /locus_tag="ACHKYO_RS01875"
                     /old_locus_tag="pgaptmp_000375"
     CDS             61850..63472
                     /gene="pckA"
                     /locus_tag="ACHKYO_RS01875"
                     /old_locus_tag="pgaptmp_000375"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HGU4427275.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63549..64901)
                     /gene="envZ"
                     /locus_tag="ACHKYO_RS01880"
                     /old_locus_tag="pgaptmp_000376"
     CDS             complement(63549..64901)
                     /gene="envZ"
                     /locus_tag="ACHKYO_RS01880"
                     /old_locus_tag="pgaptmp_000376"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HGU4427276.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64898..65617)
                     /gene="ompR"
                     /locus_tag="ACHKYO_RS01885"
                     /old_locus_tag="pgaptmp_000377"
     CDS             complement(64898..65617)
                     /gene="ompR"
                     /locus_tag="ACHKYO_RS01885"
                     /old_locus_tag="pgaptmp_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HGU4427277.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65845..66321
                     /gene="greB"
                     /locus_tag="ACHKYO_RS01890"
                     /old_locus_tag="pgaptmp_000378"
     CDS             65845..66321
                     /gene="greB"
                     /locus_tag="ACHKYO_RS01890"
                     /old_locus_tag="pgaptmp_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HGU4427278.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66418..68739
                     /gene="yhgF"
                     /locus_tag="ACHKYO_RS01895"
                     /old_locus_tag="pgaptmp_000379"
     CDS             66418..68739
                     /gene="yhgF"
                     /locus_tag="ACHKYO_RS01895"
                     /old_locus_tag="pgaptmp_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGU4427279.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAVLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69177..69404
                     /gene="feoA"
                     /locus_tag="ACHKYO_RS01900"
                     /old_locus_tag="pgaptmp_000380"
     CDS             69177..69404
                     /gene="feoA"
                     /locus_tag="ACHKYO_RS01900"
                     /old_locus_tag="pgaptmp_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HGU4427280.1"
                     /translation="MQYTPDTAWKITGFSHEISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69421..71742
                     /gene="feoB"
                     /locus_tag="ACHKYO_RS01905"
                     /old_locus_tag="pgaptmp_000381"
     CDS             69421..71742
                     /gene="feoB"
                     /locus_tag="ACHKYO_RS01905"
                     /old_locus_tag="pgaptmp_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HGU4427281.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71742..71978
                     /gene="feoC"
                     /locus_tag="ACHKYO_RS01910"
                     /old_locus_tag="pgaptmp_000382"
     CDS             71742..71978
                     /gene="feoC"
                     /locus_tag="ACHKYO_RS01910"
                     /old_locus_tag="pgaptmp_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HGU4427282.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72181..73059
                     /gene="rpnA"
                     /locus_tag="ACHKYO_RS01915"
                     /old_locus_tag="pgaptmp_000383"
     CDS             72181..73059
                     /gene="rpnA"
                     /locus_tag="ACHKYO_RS01915"
                     /old_locus_tag="pgaptmp_000383"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HGU4427283.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(73088..73858)
                     /gene="bioH"
                     /locus_tag="ACHKYO_RS01920"
                     /old_locus_tag="pgaptmp_000384"
     CDS             complement(73088..73858)
                     /gene="bioH"
                     /locus_tag="ACHKYO_RS01920"
                     /old_locus_tag="pgaptmp_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HGU4427284.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            73896..74579
                     /gene="gntX"
                     /locus_tag="ACHKYO_RS01925"
                     /old_locus_tag="pgaptmp_000385"
     CDS             73896..74579
                     /gene="gntX"
                     /locus_tag="ACHKYO_RS01925"
                     /old_locus_tag="pgaptmp_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HGU4427285.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIVSVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAAAV
                     QVWCLCRTL"
     gene            74638..75213
                     /gene="nfuA"
                     /locus_tag="ACHKYO_RS01930"
                     /old_locus_tag="pgaptmp_000386"
     CDS             74638..75213
                     /gene="nfuA"
                     /locus_tag="ACHKYO_RS01930"
                     /old_locus_tag="pgaptmp_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HGU4427286.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            75574..76890
                     /gene="gntT"
                     /locus_tag="ACHKYO_RS01935"
                     /old_locus_tag="pgaptmp_000387"
     CDS             75574..76890
                     /gene="gntT"
                     /locus_tag="ACHKYO_RS01935"
                     /old_locus_tag="pgaptmp_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU4427287.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(76935..79019)
                     /gene="malQ"
                     /locus_tag="ACHKYO_RS01940"
                     /old_locus_tag="pgaptmp_000388"
     CDS             complement(76935..79019)
                     /gene="malQ"
                     /locus_tag="ACHKYO_RS01940"
                     /old_locus_tag="pgaptmp_000388"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HGU4427288.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79029..81422)
                     /gene="malP"
                     /locus_tag="ACHKYO_RS01945"
                     /old_locus_tag="pgaptmp_000389"
     CDS             complement(79029..81422)
                     /gene="malP"
                     /locus_tag="ACHKYO_RS01945"
                     /old_locus_tag="pgaptmp_000389"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HGU4427289.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            82034..84739
                     /gene="malT"
                     /locus_tag="ACHKYO_RS01950"
                     /old_locus_tag="pgaptmp_000390"
     CDS             82034..84739
                     /gene="malT"
                     /locus_tag="ACHKYO_RS01950"
                     /old_locus_tag="pgaptmp_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HGU4427290.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(84782..85798)
                     /gene="rtcA"
                     /locus_tag="ACHKYO_RS01955"
                     /old_locus_tag="pgaptmp_000391"
     CDS             complement(84782..85798)
                     /gene="rtcA"
                     /locus_tag="ACHKYO_RS01955"
                     /old_locus_tag="pgaptmp_000391"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HGU4427291.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(85802..87028)
                     /gene="rtcB"
                     /locus_tag="ACHKYO_RS01960"
                     /old_locus_tag="pgaptmp_000392"
     CDS             complement(85802..87028)
                     /gene="rtcB"
                     /locus_tag="ACHKYO_RS01960"
                     /old_locus_tag="pgaptmp_000392"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HGU4427292.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADERQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IEMAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        20774 a        23785 c        21984 g        20526 t
ORIGIN      
        1 gatcgcgccg tccatctgag cagcaccggt gatcatgttt ttaacatagt cggcgtgccc
       61 cgggcagtct acgtgtgcgt agtgacgggt cggggtgtca tattcaacgt gagaagtgtt
      121 gatggtgata ccacgagctt tttcttccgg cgcgttatcg atctggtcga atgcacgagc
      181 agcaccgccg taggttttag ccagtacggt ggtgattgca gcggtcagag tagttttacc
      241 gtggtcaacg tggccgatag taccaacgtt aacgtgcggt tttgtacgtt caaatttttc
      301 tttagacacg gctatattcc ttactatagt gctctcccct tcaggagaga gcacgggact
      361 ttggtattaa cccttaggct tatttaccac gggcttcaat tacggcctga gcaacgttac
      421 tcggcgcttc atcatacttc aggaattcca tagtgtatga tgcacgacct ttggtcagag
      481 aacgcagctg agttgcgtat ccgaacattt cagacagcgg tacttcagcg tggatcttaa
      541 cgccagtaac ttcagattcc tgacctttga gcataccacg acgacggctc aagtcaccga
      601 taacgtcacc ggtgttctct tccggagttt ctacttcaac cttcatgatc ggctcaagca
      661 gaactggttt cgctttctta aagccttctt taaaggcgat agaagcagcc agtttaaacg
      721 ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg cagacgaata cccatgtcta
      781 ctaccgggta gcctgccagc ggacctgctt tcagctgttc ctggatacct ttatcaacgg
      841 ccgggatgta ttcgccaggg attacaccac ctttaatgtc gttgatgaac tcgtagcctt
      901 tcgggtttga acccggctcc agcgggtaca tgtcgataac aacatgacca tactgaccac
      961 gaccaccaga ctgtttcgcg tgtttacctt caacatcggt aactttctgg cggatagttt
     1021 cacggtaagc aacctgcggt ttacctacgt tcgcttcaac gttgaattca cgcttcatac
     1081 ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc gataatggtc tggttagatt
     1141 cttcgtcagt ccatacacgg aaagacgggt cttctttagc cagacggccc agagccagac
     1201 ccattttttc ctggtcagct ttggttttcg gttcaactgc gatggagatt accggctcag
     1261 ggaattccat acgttccaga atgatcggcg catccgggtc acacagggtg tcaccagtgg
     1321 ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc gcgaacttct ttgatctctt
     1381 cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg ctcacgtgca gctttcacgg
     1441 agttcagtac ggtatcacca gagttaacca caccggagta aacacggaag aaggtcaggt
     1501 taccaacaaa cgggtcggta gcgattttga acgccagtgc agagaacggc tcgtcatcac
     1561 ttgcgtgacg ctcagccgga gtgtctttac cgtcgtccag gataccgttg atcgcaggta
     1621 cgtcaaccgg ggatggcagg taatcaatta ccgcatccag catcgcctga acacctttgt
     1681 tcttgaacgc agaaccacag gttaccagga tgatttcgtt gttcagaacg cgctgacgca
     1741 gagcaccttt gatttctgct tcagtcagtt cttcaccacc caggtatttt tccatcagct
     1801 cttcagaagc ttcagctgcg gattcgatca ggttctggtg ccattcgtta gccagttcaa
     1861 ccatgtctgc cgggatatct tcgtattcga aggttacgcc ctggtcagcg tcgttccagt
     1921 tgatagcttt cattttcacc aggtcaacaa caccggtgaa atgttcttca gcaccaatcg
     1981 ccagctgcag cggaaccggg ttcgcgccca gacgggtttt gatctggtta acaactttca
     2041 ggaagttcgc acccatgcgg tccattttgt taacgaacgc aatgcgcgga actttatatt
     2101 tgtttgcctg acgccatacg gtttcagact gcggctgaac accaccaact gcgcagtaaa
     2161 ccattaccgc accatcgaga acacgcatgg aacgttctac ttcgattgtg aagtcaacgt
     2221 gccccggggt gtcgatgatg ttgatgcgat gcggctcata ctgcttagcc ataccagacc
     2281 agaatgcagt agtcgcagcg gaagtgatgg taataccacg ttcctgctcc tgctccatcc
     2341 agtccatggt tgcagcgccg tcatgaactt caccgatttt atggtttaca ccggtgtaga
     2401 acagaatacg ttcggtagta gtggttttac cggcgtcgat gtgcgcactg ataccgatat
     2461 tacggtagcg tgcgatgggt gttgtacgag ccatttgttt cctcgtttat cttttaggcg
     2521 ttcaatttaa gtagcccaaa gcgggctgct tactggaagc gcccgcctgg tgactaaaac
     2581 tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt ggcttcggcc atacggtgaa
     2641 cgtcttcacg tttcttaact gcagtacctt tgttttctgc agcatcagaa agttcgttcg
     2701 ccaggcgcag agccatggat ttatcaccgc gtttacgagc agcttcaacg atccaacgca
     2761 ttgccagagc attacgacga accggacgga cttcaactgg tacctgataa gtagaaccac
     2821 caacgcggcg agacttaact tctacagtcg ggcgcacgtt ttcgagagct acttcgaatg
     2881 cttccagttc agatttacca gagcgctgag ccagggtctc cagcgcgctg tatacgatag
     2941 attcagcagt agatttttta ccatctacca tcaggatatt tacaaattta gccagcagtt
     3001 ctgatccgaa cttcggatcc ggcagaattt tacgctgacc aatgacgcga cgacgtggca
     3061 tggaaatact ccgttgttaa ttcaggattg tccaaaactc tacgagttta gtttgacatt
     3121 taagttaaaa cgtttggcct tacttaacgg agaaccatta agccttagga cgcttcacgc
     3181 catacttgga acgagcctgc ttacggtctt taacgccgga gcagtcaagc gcaccacgta
     3241 cggtgtggta acgaacaccc gggaggtctt taacacgacc gccacggatc aggatcacgg
     3301 agtgctcctg caggttgtga ccttcaccac cgatgtagga agtcacttcg aaaccgttag
     3361 tcagacgaac acggcatact ttacgcagcg cggagttcgg ttttttagga gtggtagtat
     3421 atacacgagt acatacgcca cgtttttgcg ggcatgcttc cagcgcaggc acgttgcttt
     3481 tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt aactgttgcc attaaatagc
     3541 tcctggtttt agcttttgct tcgtaaacac gtaataaaac gtcctcacac aatatgagga
     3601 cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat atacaacgat cccgccatca
     3661 ccaggccatc tggctggagt gcttaaccgt aagtctgacg aaatcagtat agtcaatggg
     3721 aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca ataaggtctt cgttcagggc
     3781 atagacctta atgggggcat tacgcagact ttcaaggtag cggttaccgt caactgcggc
     3841 agttacgcca tcttgcaata atagcagttc gtctccttca ctgagcagac gcagcagcgc
     3901 agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc agcataaacg cctcaaaacc
     3961 tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc atctgcttcg agcggcgtgg
     4021 cttcgacaac aaagggtgtc tgcggatcta acccgcgttc gcgcagtgaa gccgcacaaa
     4081 cccagcactg ttcaatgtcg tacagaccca acaatttaaa agtggcaatg taatcacgcg
     4141 ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa aacgccatca gcaataaaga
     4201 agacggccag atcgtcagtt aatgcggaag ttgccagtaa agcatctaaa ccttcccgac
     4261 cagcagctgt accatgaggt gcagtagaaa aaacaaacgc aattcgtttc atcagaactg
     4321 taccaccctg tcacaggtca gcgaggcttc cgccagcgcg ccaagtccgc ttaaggtaaa
     4381 tcccggctga aggtttgacg aagccagccc cagccttcct gcttcagttt catcgacgat
     4441 accgcggcgt aacgctgccg ctacgcagat attcagcgcc acaccatgtt gcgcattcaa
     4501 ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc ggtgaggtca attggttagc
     4561 gttatagacc ccttcccgat agaaaaagac gctgcttaac tcatggcctt ctactatcag
     4621 cgcctgcgca aactgaaaag cactactcgc ctgttgcgta ccgtatgctg gcccggtcac
     4681 cacgatggca aaacgcatta cttatcttgc ccctggaaat caccgctttt gaactggcgg
     4741 atgtagagat agacagtgtg tttggagatg ttcaggcggt cagcaacctg gttgatcgcg
     4801 tctttaatat cgaagatccc tttctcgtag agattcagca cgatctgacg atttttggcg
     4861 ttattagaaa cattgcgatc ggcattcact tcttcgatgg tgaactccag cgtttgggta
     4921 accagatctt caacagaaga ggcaaagttg acgcttgaac cgacatccgg ggtttctggc
     4981 ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa gattcatatt gatgcacagc
     5041 agaccaatta cacgctgttc gcggttacga atcgcgatag tcagggactt cattaatacg
     5101 ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc tatccgctcc cgtcatatcg
     5161 tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa tcttccggcc tgtatgttca
     5221 ccgttagcaa tgcgaatggc tgaacatttt agatcctgca aagagtgcaa aacgatttca
     5281 cagtgggagc caataagcat cgctaacccg tccaccaccg cttcgtagga tttcagaata
     5341 tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat ccaactcact ggtttcgttg
     5401 gttaaaagcg acctggacat gaaaaaaaac actccttttc aggagcctgt cgttaacttt
     5461 tcagggcagg ctcattaatg atgcgggtaa ctaaattaat acagcggagg ttccgctttc
     5521 cagcactaat tatatccggc ctgtaataaa aaaaccgccg cctggtcagg cggcggttct
     5581 taatgcttat tttttagcag aatctgcggc tttcgcatca gcttccggct ttgcatcagc
     5641 cttcggcgct ggtttcacgt ccaacagctc tacgtcaaac accagggtag aattcggtgg
     5701 gatccccgga acacccgctt tgccgtaagc cagttctggt ggaataacca gtttgatctt
     5761 accgcctttc ttgatgttct tcagaccttc tgtccaaccc gggataacac cgtccagacg
     5821 gaaagagagc ggttcaccac gagtgtaaga gttgtcgaac tctttaccgt cgatcagagt
     5881 acctttgtag ttcacgacaa cagtatcgct gtctttcggt gcttcgcctt taccggcttc
     5941 tactacctga taaaccagac cagttgaaga ggttttcaca cctttctctt tggcaaattt
     6001 ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg tctttttcca tcttcgcctg
     6061 agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc tgttcgatct cttggtcgga
     6121 gagtttgctc ttatcagcaa atgcatcctg aacaccagcg atcagctgat ctttatccag
     6181 tttgatgccc agtttttctt gttcttttag agagttttcc atgtaacgac ccagcgaggc
     6241 acccagtgca taagctgact tctgatcgtc atttttgaac gctgctttgc tgtcagcagt
     6301 tgtagcaggt tttgcagctt cagcagcaaa agtgattggt gcatgcaggg caacggccat
     6361 tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc catatctcca ggatcggggc
     6421 atctcacccc agggttaact attatcagaa gggtactata aagcgttgtc gaacaaatct
     6481 acatacagac acgccctatt atcatctatt ttcagactct ttttgtttaa attagtttcg
     6541 atgaccgcga aatgagtgct gtctcgggca gcaaagttaa gtagaatccg cggcaaccat
     6601 tcgacaaaag aggtgaatca tgcaggattt atcattggaa gcacgcctgg cagagctgga
     6661 gagccgactg gcttttcagg aaatcaccat tgaagaactg aacgtcacgg tgaccgctca
     6721 tgaaatggag atggagaaac tgcgcgatca tctgcgtctg ctgactgaga agttaaaagc
     6781 cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg ccaccgccgc attattgagg
     6841 cgaaaaaaaa gcggggaatc cccgcttttt tgtcaccttt tcggtattag tggcaaccac
     6901 aaccgccgtt gcctttaccg ccacagcagc cttcgccacc gtgttcatga ccgtgatcgt
     6961 ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg gtggtgatcg tgcgcgccgt
     7021 gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg aatcgccaca acttcaacgt
     7081 tgaatttcag gttctgaccg gccagcatgt ggttaccatc aaccacgacg tgatcgtctt
     7141 caaccgcagt gatttcaacc ggtaccggac cctggtcagt ttcagccagg aaacgcatac
     7201 ctacctgcag ttcatcaacg cccataaata cgtctttagg aacacgttgc accaggtttt
     7261 cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac atcaaatttg tcgccaactt
     7321 catgaccttc cagcgccgtt tccaggccag agatcaggga accgtgacca tgcaggtagt
     7381 ccagcggcgc actcaccgga gactcatcaa ccaacacacc gtcttctgta cgtacctgat
     7441 aggccaggct gaccaccagg tcttttgcta ctttcatgat atctcctgag catgggaaga
     7501 atagtggcgc agattgtagc ggaattctgc agccgtgtac tcactagctt aaaaaaactc
     7561 ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt gctcatcttt gcgaacgtga
     7621 tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc acttaacgca ttcaacaata
     7681 tcaatattat tttcgcgcca catcgccatt gaatcctgcg cctgacaggc cgggcatttt
     7741 gcgcccgcaa taaaacgttt tcggattgcc atctttaccc tctactcaaa ttcatcccag
     7801 ccgtccagct ggcgtcgttc ttgttgcatt tcacgttgga aaatctcttc cagttcgcgt
     7861 cgggcttccc tggcgcgaga aatttgtacg gtatcagcat gcatcgggat taactcccgc
     7921 agcattcgca tatccaggcg gcgaaaatgc agttgcgccc gctgtgcctg atgcggatgc
     7981 atgccaagcg tgaccagcgt cttgcgcccc agctctaacg cactggagaa tgtttcacgg
     8041 gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt ccacacgtcc gcgcgctcgc
     8101 gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt ccaccagctt catggtgtct
     8161 tccggctcgt tacaggtaat gacgatagac tcagcggcct ctgcacccgc agaacgtaaa
     8221 agatcgacct gcgtggcgtc gccgtaataa actttgtagc catatttacg catcaggtta
     8281 acggcgctga tatcccgctc cagcacggta atgcgcattt tatttgccat cagcaaacga
     8341 ccaatcacct gaccaaaacg cccgaagccc acgacaatga cctggggttt atcatcgttg
     8401 acccacggtt tttcgtcttc ttcttccggt ccgttaaact ggcgggatag ccatttatcc
     8461 accagcttca tcagcagcgg cgtggtcatc atggaaagcg tcaccgtcac cagcaacagc
     8521 gccatctggt cgccctggaa taagcgttgt gaagaagcgg tagaaaagag gacaaaggca
     8581 aactcaccgc cctgactcaa cacgccagca aactgcatcc gctctgagct acgcacgcca
     8641 tacaatcgcg ccagcagata caacacgaga attttcaccg ccaccagcac aaccacgctt
     8701 atcactaccc acaacagatg ggtataaagc accccgaggt tgagcgacat gccgacagag
     8761 ataaagaaca aaccgagcag caagcctttg aagggatcga tagccgtttc cagttcatgg
     8821 cgatattcac tttccgccag cagcacgccc gcaataaacg taccgagcgc catcgacagc
     8881 cccagcgcgt ccataaacaa tgcggaaccc aacaccaaca gcagcgtcgc ggcggtgaac
     8941 acttcccgca cgccagaagc tgcaataaaa cggaataccg gacgcagtaa atagcgcccg
     9001 ccaatcagca tgccgacaaa cgccagcacc ttcatgccga tcttcatcca gtcgaaatgt
     9061 tcgtctgccg aacccgccaa caacggcact aacgccagtg ccgggatcac cgccagatcc
     9121 tgaaacagca gaaccgaaaa cccgagctgc ccggattcgc tgcgattcat ccctttctca
     9181 cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa ggccaatgcc accgacaacc
     9241 gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg ccagcaacgc cgcgcttaac
     9301 agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca gttgccaaag tttggaggga
     9361 ttcaactcaa gaccgatgat aaacatcagg aataccacgc cgagttccga aaagtggagg
     9421 atctcatcga cgtcgctaat aaaccccagc ccccatgggc caattgcaat ccctgccagc
     9481 aaatatccca acacagcgcc aatacccagc cgcgatgcca gcggcaccgc agccaccgcc
     9541 gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac cttccatcag cggcctcctg
     9601 gagacagcgg atttgccagc cagtcaccgt aggctctggc gtgactcgcc agctcctgtg
     9661 cgctttgccg tctcgcccag taaataatga tgggacttaa ccaatgcatc cggcacatgc
     9721 ccgccgccag ttcaaaggga cgcagaacat cgctcatcgg gtagcgattc aatgcgtcat
     9781 aacggtaagc actttccggc tcgccggtgg taatcacgct acgccagtac tttcccgcca
     9841 gttggtttcc tcccggcccg ctggcaaaac cacgacttaa tacccggtcc agccactctt
     9901 tcagtagcgc cgggcagcta taggtataaa gaggatgctg aaagacaatc acctcgtgct
     9961 cgcgcagtaa tgcctgctca cgggggatat caataaaaaa atcgggatag tgcgcataaa
    10021 ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt aagcagtacc cggtttgcca
    10081 ccgagtcctg agattccgga tgggcataca gcagcaaaac tttcgctggc tgagacatca
    10141 tccccctccc ggtatggttt ttgtgtatag tcgctgtttt gggctaccat tgcgcccggt
    10201 gcggcagctc gcccatacat tacattatca taatgataag ttaacatagt ctgaacatac
    10261 ggcaccttat gattgttttc tcctcgttac aaattcgtcg cggcgtgcgc gtcctgctgg
    10321 ataatgccac cgccaccatc aaccctgggc agaaagtcgg cctggtgggt aaaaacggct
    10381 gtggtaaatc taccctgctg gcattgctga aaaatgaaat cagcgccgac ggcggcagct
    10441 acacctttcc gggaagctgg caactggcgt gggtgaatca ggaaacgccg gcgttaccgc
    10501 aagcggcgct ggaatatgtc attgacggcg accgtgaata tcgtcaacta gaagcgcagc
    10561 tacacgacgc caacgaacgt aacgacgggc acgccattgc gaccattcat ggcaagctgg
    10621 atgctattga cgcatggagt attcgctccc gtgccgccag cctgctgcac ggcctcggtt
    10681 tcagcaatga acaactggag cgcccggtaa gtgatttctc cggtggctgg cgtatgcgtc
    10741 ttaaccttgc ccaggcgctg atttgccgtt cagacttgct gctgctcgac gaaccgacta
    10801 accacctcga tctcgatgcc gttatctggc tggaaaaatg gttgaagagc tatcagggca
    10861 cgctgatcct gatctctcac gaccgcgact tcctcgatcc gatcgttgat aaaattattc
    10921 atatcgaaca acaaagcatg ttcgagtaca ccggcaacta cagttcgttt gaagtacagc
    10981 gcgccacccg tctggcgcag caacaagcga tgtatgaaag ccagcaggaa cgcgtagcgc
    11041 atctgcaaag ttatatcgac cgtttccgtg ccaaagccac caaagcgaag caggcccaga
    11101 gccgcattaa gatgctcgag cgtatggagc tgattgcccc cgcgcacgtc gacaacccgt
    11161 tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc gttactgaag atggaaaaag
    11221 tcagcgcggg ctatggcgat cgcattattc tcgactcgat taaactgaac ctggtgcccg
    11281 gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa atcgacatta atcaaactgt
    11341 tagccggtga acttgcgcca gtcagcggtg aaattggtct ggcgaaaggg atcaagctcg
    11401 gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga cgaatcacct attcaacatc
    11461 tggcacgttt agcgccgcag gagctggaac aaaaactgcg tgactacctc ggcggctttg
    11521 gtttccaggg cgataaagta accgaagaaa cgcgccgctt ctcaggtggg gaaaaagccc
    11581 gcctggtgct ggcattaatt gtctggcagc gtccgaatct gctgctgctc gacgaaccga
    11641 ctaaccacct tgacctcgac atgcgtcagg cactcaccga agcattaatc gagttcgaag
    11701 gcgcgctggt tgtcgtctcg cacgaccgtc atttgctgcg ttccaccact gacgacctct
    11761 acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga tctggaagat tatcaacagt
    11821 ggttgagcga cgtacaaaag caggaaaacc agaccgacga cgcgccaaaa gagaatgcga
    11881 acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc ggagctgcgt gcgcaaaccc
    11941 agccactgcg taaagagatt gcccgtctgg aaaaagagat ggagaagctg aacgcgcaac
    12001 tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta tgaccagagc cgtaaagcgg
    12061 agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc cggcctggaa gagtgcgaaa
    12121 tggcgtggct ggaagcccag gagcagcttg agcagatgct gctggaaggc caaagcaact
    12181 gatggcgcag ataacgacga ccgatgccaa tgaattcagc agcagtgctg aattcatccc
    12241 catgcgcggc tttagcaact gccatctaca aactatgctg ccgcgtctgt ttcgtcgtca
    12301 ggtgaaattc accccgtact ggcagcggct ggagttgccc gacggcgatt ttgtcgatct
    12361 tgcgtggagt gaaaaccctg cacaggcgca acataaacct cgtctggtgg tgtttcacgg
    12421 gctggaaggc agtctcaata gcccttacgc ccacggtctg gttgaggcgg cgcaaaagcg
    12481 cggctggctg ggcgtggtga tgcattttcg cggatgcagc ggtgaaccaa accgtatgca
    12541 ccgcatttac cattcgggcg aaaccgaaga cgccagttgg tttttacgct ggctgcaacg
    12601 cgaatttggt catgcgccaa cggctgccgt cggctattcg ctcggcggta atatgctggc
    12661 ctgtttgctg gcaaaagaag gcaatgatct ccctgttgat gcggcggtga ttgtctctgc
    12721 gccgtttatg ctggaagcct gtagctatca tatggaaaag ggcttttccc gcgtttatca
    12781 gcgttacttg ctgaacctgt taaaagccaa tgccgcacgc aagctggcag cctaccccgg
    12841 aacactgccg attaatctcg cgcagttaaa atcggtacgt cgcatccgtg aatttgacga
    12901 tctaatcacc gccagaattc acggctacgc tgacgctatc gactattatc gtcagtgtag
    12961 cgccatgccg atgctgaacc ggatcgccaa acctacgctg attattcacg ccaaagacga
    13021 tccgtttatg gatcatcagg tgatcccgaa accggaaagt ctccccccgc aggttgagta
    13081 tcaactgact gaacatggcg gtcatgttgg ctttattggc ggtacattac ttcatccgca
    13141 aatgtggctg gagtcacgca ttcctgactg gttaacaacg tatctggagg cgaaatcatg
    13201 ttgattccgt ggcaagacct ctcccccgaa acgctggaaa atttaattga aagctttgtg
    13261 ttacgtgaag gcaccgatta tggtgaacat gagcgtacac ttgaacagaa agtcgccgac
    13321 gtcaaacgcc agctacagtg cggagaagcg gtgctggtat ggtcggagct gcacgaaacg
    13381 gtcaatatca tgccgcgcag tcagtttcgc gaataacctg caaccgtcgg cggacgagat
    13441 aataatgata gctaagtcat ggagttacta tgtctgccaa acatccggtc attgcggtaa
    13501 caggatccag cggcgcgggg accaccacca ccagcctcgc gtttcgtaaa atattcgcgc
    13561 agttaaatct gcatgcagct gaggtggaag gcgacagttt tcaccgttac acccgcccgg
    13621 aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg gcatatcagc tacttcggcc
    13681 ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat tgaatacggt cagagcggca
    13741 aagggaaatc tcgcaaatat ctgcatacct acgacgaagc cgtaccctgg aatcaggtac
    13801 cggggacatt caccccctgg caacctttac cggaacccac tgatgtactg ttttatgaag
    13861 gtttacacgg cggcgtagtc acgccacagc ataacgttgc gcagcatgtg gacttactgg
    13921 tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa actgatccgc gacaccagcg
    13981 agcgcgggca ctcacgagaa gcagtgatgg actcagtagt gcgttcaatg gaagactata
    14041 tcaactacat cacaccgcag ttttcccgca cccatcttaa cttccagcgc gttcccaccg
    14101 tcgacacttc aaacccattc gcggcaaaag gtatcccgtc gctcgatgaa agctttgtgg
    14161 tgatccattt tcgtaatctg gaagggatcg atttcccctg gctgctggcg atgttgcaag
    14221 gctcattcat ttcccacatc aatacgttag tggtaccggg cggcaaaatg ggtctggcaa
    14281 tggaattaat tatgctgccg ctggtgcaac gattgatgga aggaaagaaa atcgagtaac
    14341 tctgctatta cgccggataa aatactatcc ggcttcacaa cgggatagtt aaatcacgcg
    14401 gcaaccactt catacgagtg agtaatattc accgcttttt ccagcatcaa cgccactgaa
    14461 caatatttct cagcagagag atcaaccgca cgcgcaaccg ctgcgtcttt caggtcgcga
    14521 ccggtgacga taaaatgcag attaatgtgc gtaaacaggc gtggtgcctc ttcgcggcgt
    14581 tcagaggtca attttacttc acaatcgacc acatcctgac gccctttttg caggatcgaa
    14641 accacatcga tggcactgca accacccacc gccatcaaca ccatttccat cggacttggt
    14701 gctttatcgc ctgagttgcc gtccattaaa atctggtgac cagaggcgga ttcgcccaga
    14761 aaagttaacc cttcgaccca cttcactcgc gcttgcattt ttgctactcc actgcgtcaa
    14821 ttttcctgac agagtacgcg tactaaccaa atcgcgcaac ggaaggcgac ctgggtcatg
    14881 ctgaagcgag acaccaggag acacaaagcg aaagctatgc taaaacagtc aggatgctac
    14941 agtaatacat tgatgtactg catgtatgca aaggacgtca cattaccgtg cagtacagtt
    15001 gatagcccct tcccaggtag cgggaagcat atttcggcaa tccagagaca gcggcgttat
    15061 ctggctctgg agaaagctta taacagagga taaccgcgca tggtgcttgg caaaccgcaa
    15121 acagacccga ctctcgaatg gttcttgtct cattgccaca ttcataagta cccatccaag
    15181 agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt actacatcgt taaaggctct
    15241 gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga tcctctccta tctgaatcag
    15301 ggtgatttta ttggcgaact gggcctgttt gaagagggcc aggaacgtag cgcatgggta
    15361 cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca aaaaatttcg ccaattgatt
    15421 caggtaaacc cggacattct gatgcgtctg tctgcacaga tggcgcgtcg tctgcaagtc
    15481 acttcagaga aagtgggcaa cctggcgttc ctcgacgtga cgggccgcat tgcacagact
    15541 ctgctgaacc tggcaaaaca accagatgct atgactcacc cggacggtat gcaaatcaaa
    15601 attacccgtc aggaaatcgg tcagattgtc ggctgttctc gtgaaaccgt gggacgcatt
    15661 ctgaagatgc tggaagatca gaacctgatc tccgcacacg gtaaaaccat cgtcgtttac
    15721 ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta gcgcgccatt ttgtttcccc
    15781 cgatgtggcg cagactgatt tatcaccccg atatcaacta tgcacttcga caaacgctgg
    15841 tgctatgttt gcccgtggcc gttgggttaa tgcttggcga attacgattc ggtctgctct
    15901 tctctctcgt tcctgcctgt tgcaatattg cgggccttga tacgcctcat aaacgttttt
    15961 tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg tagcttactg acacagctgc
    16021 tactggcaaa agatgttccc ctgccctttt tgctgaccgg attaacgctg gtacttggcg
    16081 tcactgctga gttggggcca ttgcacgcaa aattgcttcc cgcatcgctg ctggcagcca
    16141 tttttaccct cagtctggcg ggatacatgc cggtctggga accgttgctc atctatgcgt
    16201 tgggcactct ctggtacgga ttgtttaact ggttttggtt ctggatctgg cgcgaacaac
    16261 cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc agattattgt gaagccaaat
    16321 acagcctgct tacccagcac accgaccctg aaaaagcgct gccgccgctg ctggtgcgcc
    16381 agcaaaaagc ggtcgatcta attacccagt gctatcagca aatgcatatg ctttccgcgc
    16441 aaaataatac tgactacaag cggatgctgc gtattttcca ggaggcgctg gatttacagg
    16501 aacatatttc ggtcagtttg catcagccgg aagaggtgca aaagctggtc gagcgtagcc
    16561 atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc cgctcgcctg cgcgtgctgg
    16621 ctgatgacat tctttaccat cgcctgccaa cgcgttttac gatggaaaag caaattggcg
    16681 cactggaaaa aatcgcccgc cagcatccgg ataatccggt tgggcaattc tgctactggc
    16741 atttcagccg catcgcccgc gtgctgcgca cccaaaaacc gctctatgcc cgtgacttac
    16801 tggccgataa acagcggcga atgccattac ttccggcgct gaaaagttat ctgtcactaa
    16861 agtctccggc gctacgcaat gccggacgac tcagtgtgat gttaagcgtt gccagcctga
    16921 tgggcaccgc gctgcatctg ccgaagtcgt actggatcct gatgacggta ttgctggtga
    16981 cacaaaatgg ctatggcgca acccgtctga ggattgtgaa tcgctccgtg ggaaccgtgg
    17041 tcgggttaat cattgcgggc gtggcgctgc actttaaaat tcccgaaggt tacaccctga
    17101 cgttgatgct gattaccacc ctcgccaact acctgatatt gcgcaaaaac tacggctggg
    17161 cgacggtcgg ttttactatt accgcagtgt ataccctgca actattgtgg ttgaacggcg
    17221 agcaatacat ccttccgcgt cttatcgata ccattattgg ttgtttaatt gctttcggcg
    17281 gtactgtctg gctgtggccg cagtggcaga gcgggttatt gcgtaaaaac gcccatgacg
    17341 ctttagaagc ctatcaggaa gcgattcgct tgattcttag cgaggatccg caacctacgc
    17401 cactggcctg gcagcgaatg cgggtaaatc aggcacataa cactctgtat aactcattga
    17461 atcaggcgat gcaggaaccg gcgtttaaca gccattatct ggcagatatg aaactgtggg
    17521 taacgcacag ccagtttatt gttgagcata ttaatgccat gaccacgctg gcgcgggaac
    17581 accgggcatt gccacctgaa ctggcacaag agtatttaca gtcttgtgaa atcgccattc
    17641 agcgttgtca gcagcgactg gagtatgacg aaccgggtag ttctggcgat gccaatatca
    17701 tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg tacgctggag cagcatttac
    17761 agcgggttat tggtcatctg aacaccatgc acaccatttc gtcgatggca tggcgtcagc
    17821 gaccgcatca cgggatttgg ctgagtcgca agttgcggga ttcgaaggcg taatgcaggc
    17881 taaaccatcg gataaggcat tcacgccgca tccgacatct tttgcctgat gcttcgtatt
    17941 tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt gcatcccttc atcgatatcc
    18001 gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat ccggtccggc attcagcacc
    18061 attacgccag cccctgcgcc cgcatacagg aaatcacgcg cccgaccttt gaactgtggc
    18121 ttaagctccg cgccaatcaa cagccccata ccgcgaatat cgctaaacac atcgtactgc
    18181 tgatcgatct tctgcagatg gtcaacaaaa cgctggcgtt tcgcctgaat gccttccagc
    18241 acttcagggg tattgatgat atcaaacgcc gcgcccgcta ctgcacagga cagaggatta
    18301 ccgccgtagg tggaaccgtg agaaccagga tgaaatgcag aagcaatttc tgccgtggtc
    18361 agcatggcgc taatcgggaa gccgccgcct aacgctttcg cagaggtcag aatatccggc
    18421 gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc gccccatccc gcactgtact
    18481 tcatcaaaca ccaataatgc ctgatgttga tcgcacagct cgcgcaagcc ctgcaaaaac
    18541 tctggcgtcg ctgccgtcac accgccctcg ccctggatcg gctcaaccac caccgcacag
    18601 gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt taaaaggaac gtggatgatg
    18661 tctgccggtt ttggcccaaa gccgtcggaa tattttggct gcccgcccac cgagacggta
    18721 aacagcgagc gaccatgaaa agcgttatgg aaggcaataa ttttggtttt gaacggacta
    18781 tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg tttcgttagc ttccgtgccg
    18841 gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa tcagtttgcg cccaagacgc
    18901 agcgccggtt cattggtgaa aacgttactg atatgccaca gagtttcgcc ctgggttttt
    18961 aacgcgttca ccagcgcagg atggcaatgg cccaacgccg taactgcaat gccacccgcg
    19021 aaatcgacat actccttgcc ttgctgatcc cagattcggc tgccctgacc ttttaccgga
    19081 atgaactctg ccggagcata aatcggcagg atcacttcat cgaaagtcgc gcgtgtaatt
    19141 gctgtttgtt caattgccat ctcatgatca ccctgttacg cataaataaa tgtgaaatta
    19201 taatcacaaa atatgcataa aaaatcattg aatggcaatc agaaatcagc gatgcaggaa
    19261 attagccagc agttgatgtc cttgttcgct aagaatactt tctggatgga actgcacacc
    19321 ttccagatcc cactggcgat ggcgaatccc cataatctcg cgggtttcgc tccaggccgt
    19381 cacgtcaaag catgctggta atgagccaag ttccaccacc agcgaatggt agcgtgtcac
    19441 ggtaagtgga tttgccagcc cccgaaatac gccctcaccg ttatgtgtaa tcggcgaggt
    19501 tttgccgtgc atgacctttg cggcgcgcac aactttgccg ccaaatgcct gcgccattgc
    19561 ctgatgaccg aggcagacgc caagaatcgg caagcgcccg gcatagtggc gaataacgtc
    19621 aagggagatc ccggcttcat ctggcgtaca ggggccgggt gatatgacaa ttttttgtgg
    19681 tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg cgcttaacca gcacatccgc
    19741 ccccagttca caaaagtact ggtagaggtt ccaggtaaaa gaatcgtagt tatctataag
    19801 caggatcatg gcggctccgg tacgaaagaa ccgcgctatt ttactcagat tccccggctt
    19861 cgcttaccac tttgctaaat atcatctgca gcgcggtgag atcccccatt gccccactct
    19921 gatttgcctg attccaggct tctttttcga taccctgcca gctcagttta tatccggcat
    19981 gaatcgccag ttgctcgaag aagatccgct gtgccagtcc gcttcccaca cggaagggat
    20041 gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc gacgaacttc gctttctcca
    20101 ggccaaccag ataaccttct tcctccagat cctgcatcag ggcattgccc tctttttcga
    20161 tataagcaaa gtggcagaac ggcgtatcgc cctgataaat atcaacttca cgcagttgcc
    20221 ccgcccagtc gaaaatatcc tgatacagct ggcgatggat agtgcgcaaa tgcggtaaac
    20281 cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag cgccgtcatt tcgtaagcgg
    20341 cctgttccag ccgctgctgc tggtggatgt tcagccggtt acgcatgata tcaaggccag
    20401 gataaagata cggatcgcgc ccttcgccga atttatcgct catagtgcct cctcagctct
    20461 tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta aaggcatctc gacgccttca
    20521 agacggcgac tggcctggaa attacgatta cgctgaagct cccagagacg ggacttttgc
    20581 ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg tccatttgcc acaagtataa
    20641 gcggcaaatc cgggttacgc cgggagagca gaagcgcggg caagattgcc cgcgcgaaaa
    20701 atcattacgg caggacttta gcggaaagga taactaccgg ttttgacggc acattctggt
    20761 acggaccaac gtcatgagtc ggcacctggg aaatcttatc ggcaacgtcc atgcctttca
    20821 ccactttacc aaataccgcg taaccgaaat cacgctgacc atggtcaagg aaggcgttat
    20881 cggcaacgtt gataaagaac tggctggtgg cgctgtcttt gtcagcggta cgcgccatcg
    20941 cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc atttttgatt ggcgggtttg
    21001 gttttttctg ctgcatctgc tcggtgaaac cgccgccctg aatcataaag ccaggaatga
    21061 cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac ataatcgaca aagttttgca
    21121 cagacactgg cgctttttgt ttatccagct ccagttcgat gttaccagct gaggttgtca
    21181 acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg agaaagagca gaaagagcga
    21241 aaacagcagc catcgccgcc agggtcgatt tgaacatgag atttccttaa caaacagagc
    21301 agaaaaaaag caagtgaaga gattctaaat agccttcaag aacaaggcca tccctttacc
    21361 taattttacg tatctgaaat atatgtaacc tgacgatcac tttttattcc gtgatcaaaa
    21421 tcacctctta aaatgcaatt tagcaaccga ttgcaataaa acatttaaac agatcacaaa
    21481 atcacctaaa atcgcccgct cgcggcatat cccgctatgc cattttattt ttgaacgcag
    21541 gccaatcatg actaacagca atcgcatcaa gctcacatgg attagctttc tctcctacgc
    21601 actgactggt gcgttggtta ttgtcaccgg gatggtgatg ggaaatatcg ccgattattt
    21661 caatctgcct gtttccagta tgagtaacac cttcaccttc ctcaacgccg gcattttaat
    21721 ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg ttgaaaacgc agttacgttt
    21781 tggctttctc ctgatggtgc tggcggttgc cggtttgatg ttcagccaca gcctggcgct
    21841 gttctcggcg gcgatgttca ttctcggggt ggtcagcggc atcaccatgt cgattggtac
    21901 attcctggta acacaaatgt atgaagggcg tcagcgcggt tcccgcctgt tatttaccga
    21961 ctccttcttc agtatggctg ggatgatttt cccaatgatc gccgcgtttc tactggcgcg
    22021 cagcattgag tggtactggg tttatgcctg catcgggctg gtgtatgtcg ctatttttat
    22081 tctgaccttc ggctgtgagt tcccggcgct gggcaaacat gcgccaaaaa cggatgctcc
    22141 ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc tctgttgcgg cgctgtgcta
    22201 catcctcggt cagttaggtt ttatctcctg ggtgcctgag tatgccaaag gcctgggcat
    22261 gagcctgaac gacgcgggca cgctggtgag taacttctgg atgtcataca tggtcggcat
    22321 gtgggcgttc agctttattc ttcgcttctt tgatttgcaa cgcattctga ccgtactggc
    22381 tggtctggct gcgattctga tgtacgtctt taacaccgga acaccagcac atatggcgtg
    22441 gtcaattctc gctctgggct tcttctccag cgcgatctat accaccatca tcaccctggg
    22501 ttcacagcag accaaagtac cgtcgccaaa actggttaac tttgtcctga cctgcggaac
    22561 catcggtact atgttgacct ttgtggttac cggcccgatt gttgaacata gcggtccgca
    22621 ggcggcactg cttacagcaa acggtctgta cgctgccgtc tttgtgatgt gcttcctgtt
    22681 aggtttcgtc agccgtcacc gtcagcataa caccctgacc tctcattaat tgctaatgcc
    22741 ggacggcact atcgtcgtcc ggccttttcc tctcttcccc cgctacgtgc atctatttct
    22801 ataaacccgt tcattttgtc tattttttgc acaaacatga aatatcagac aattccgtga
    22861 cttaagaaaa tttatacaaa tcagcaatat accccttaag gagtatataa aggtgaattt
    22921 gatttacatc aataagcggg gttgctgaat cgttaaggta ggcggtaata gaaaagaaat
    22981 cgaggcaaaa atgagcaaag tcagactcgc aattatcggt aacggtatgg tcggccatcg
    23041 ctttatcgaa gatcttcttg ataaatctga tgcggccaac tttgatatta ccgttttctg
    23101 tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg tcttacttct ctcaccacac
    23161 cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag aaacacggca tcaaagttct
    23221 ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag gtgattcact ccagcgccgg
    23281 acgtaccgtt ttttatgaca agctgatcat ggcaaccggt tcctacccgt ggatcccgcc
    23341 aatcaaaggt tctgatactc aggactgctt tgtctatcgc accattgaag acctcaacgc
    23401 cattgaatcc tgcgcccgtc gcagcaagcg cggtgccgtt gttggtggcg gcctgttagg
    23461 tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa acccacgtta tcgaatttgc
    23521 ccctatgctg atggcggaac agcttgatca gatgggtggc gagcagctgc gtcgcaaaat
    23581 cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc cttgagattg tgcaggaagg
    23641 tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc gaactggaag tcgactttat
    23701 cgtcttctct accggtatcc gtccgcgcga taagctggca acccagtgtg gtctggacgt
    23761 tgctccgcgt gggggtattg tcattaatga ttcctgccag acttccgatc cggatatcta
    23821 cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt ggtctggtag caccaggcta
    23881 caaaatggcg caggtcgccg ttgaccatat tctcggtagc gaaaacgcct ttgaaggtgc
    23941 tgaccttagc gccaagctga aactgctggg cgtagacgta ggcggtattg gtgatgcgca
    24001 cggtcgcacg cctggcgcac gtagctacgt ttacctcgac gaaagtaaag agatctacaa
    24061 acgcctgatt gtcagcgaag acaacaaaac cctgctcggt gcggtactgg tgggcgatac
    24121 cagcgactac ggtaacctgc tgcaactggt gctgaacgct atcgaactgc cggaaaaccc
    24181 ggattccctg atcctgccag cacactctgg tagcggcaag ccgtctatcg gtgttgataa
    24241 actgccggac agcgcgcaaa tctgctcctg cttcgacgtc accaaaggtg atctgattgc
    24301 tgccatcaac aaaggctgcc acaccgttgc ggcgctgaaa gctgaaacca aagcgggtac
    24361 tggctgcggt ggctgtatcc cgctggtcac tcaggtactg aacgcggaac tggcgaaaca
    24421 gggcatcgaa gttaacaaca acctgtgcga acactttgct tattcgcgtc aggaactgtt
    24481 ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa ctgctggcga aacacggcaa
    24541 aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg ctgctggcct cctgctggaa
    24601 cgaatacatt ctgaagccgg aacatactcc gctgcaggat tctaacgaca acttcctcgc
    24661 taacatccag aaagacggaa cctactcggt gatcccgcgc tctccgggcg gtgaaatcac
    24721 cccggaagga ctgatggcag tgggtcgtat cgcgcgtgaa tttaatctct acaccaaaat
    24781 caccggctct cagcgcctgg cgatgtttgg cgcacagaaa gacgatctgc cggagatctg
    24841 gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc tatgcgaaag cactacgtat
    24901 ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc gttggcgaca gcgtcggcct
    24961 cggcgtggaa ctggaaaacc gctacaaagg catccgtacg ccgcacaaaa tgaagttcgg
    25021 tgtctccggc tgtacccgtg aatgttcaga agctcagggt aaagacgtgg gtattatcgc
    25081 cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc ggcatgaaac cgcgtcatgc
    25141 ggatctgctg gcagcggata tcgatcgcga aacgctgatc aaatatctcg accgcttcat
    25201 gatgttctac atccgtaccg ccgacaaact gacgcgtacc gcgccgtggt tagaaaacct
    25261 cgaaggcggc atcgattatc tgaaagcggt gatcattgac gacaaactgg ggctgaacgc
    25321 acatctggaa gaagagatgg cgcgcctgcg tgaagcggta gtgtgtgagt ggactgaaac
    25381 ggtcaataca ccgtccgcgc agactcgctt caaacacttc atcaacagcg acaagcgtga
    25441 cccgaacgta cagatggtgc cagagcgcga acagcaccgt ccggcaacgc cgtatgaacg
    25501 tatcccggta actctggtgg aggacaacgc atgagccagt ggaaagacat ctgcaaaatc
    25561 gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag gtgacgagca ggtagcaatt
    25621 ttccgcccgt atcacagcga tcaggtgttt gcgatcagca acatcgaccc gttcttcgag
    25681 tccagcgtgc tgtcacgcgg gctgattgcg gaacaccagg gcgagctgtg ggtcgccagc
    25741 ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt gcatggaaga cgaacagttt
    25801 tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg tgcagctgcg cggttaatgt
    25861 tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc tgtaatgacc ttcggtatat
    25921 tgcaggacat tttttaaact ttttgtttta tttttttgtt tttatttttt aaaggataat
    25981 caaatgttta cagacactat taataagtgt gcggctaacg ctgcgcgcat tgcacgcctg
    26041 tcggcaaata acccgctcgg cttttgggtc agctccgcca tggcgggcgc gtatgtgggt
    26101 cttgggatca tcttgatttt cacgctcggt aatttgctcg acccgtccgt acgtccgctg
    26161 gtgatgggcg cgacctttgg tatcgcctta acgctggtga ttatcgccgg ttctgaactg
    26221 ttcaccggac acaccatgtt cctcaccttt ggggtaaaag cgggcagcat cagccacggg
    26281 caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc tggtcggttc cgtcttcgtt
    26341 gccatgctct atagctgggg cggcggtagc ctgctgccgg tagataccag catcgttcac
    26401 tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac tgttcttcaa aggtgcattg
    26461 tgtaactggc tggtttgcct ggcaatctgg atggcgctgc gcactgaagg ggcggcgaaa
    26521 tttatcgcta tctggtggtg tctgctggca tttatcgcgt ctggctacga gcactctatc
    26581 gctaacatga cgctgttcgc gctctcctgg ttcggcaacc acagcgaagc ctacacgctg
    26641 gcgggtattg gtcataacct gctgtgggtg acgctgggta atactttatc aggtgccgta
    26701 ttcatgggat tgggttattg gtatgctacg ccgaaagcga atcgtccggt tgcggacaaa
    26761 tttaatcaaa ctgaaacggc tgccggttaa ttactaaggg gtttttacgt ggatcatttg
    26821 cctatatttt gccaattacg cgatcgcgac tgtctgattg tcggcggtgg tgatgtcgcg
    26881 gaacgcaaag caaggttgct gttagacgca ggcgctcgct taacggtgaa tgcattagcg
    26941 tttattccac agttcaccgc atgggcagat gcaggcatgt taaccctcgt cgaagggcca
    27001 tttgatgaaa gccttctcga cacctgctgg ctggcgattg cagcgacgga tgatgacgcg
    27061 cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca tcttctgtaa cgtggtcgat
    27121 gcgccgaaag ccgccagctt tattatgccg tcgattattg accgctcacc gctcatggtc
    27181 gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc tgttgcgcga aaaactcgaa
    27241 tcactgctgc cgttgcatct gggccaggtg gcgaaatatg ccgggcaatt acgcgggcga
    27301 gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt tctgggagaa attgttcgtt
    27361 aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga aagccattac tgaaacgacc
    27421 gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag tggtgctggt tggtgcaggt
    27481 ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac aaattcagca ggcagatgtg
    27541 gtggtctacg accgtctggt ttctgacgat attatgaatc tggtacgccg cgatgctgat
    27601 cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac cgcaggaaga gattaaccag
    27661 atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc ggctgaaagg tggcgatccg
    27721 tttatttttg gccgtggtgg cgaagagctg gaaacactgt gcaacgcggg tattccgttc
    27781 tcggtggttc cgggtattac cgcagcttct ggttgctctg cctattcggg tattccgctc
    27841 acgcatcgcg attatgccca gagcgtgcgc ttaattaccg gacacttaaa aaccggtggc
    27901 gagctggact gggaaaacct ggcggcagaa aaacagacgc tggtgttcta tatggggctg
    27961 aatcaggctg cgactattca gcaaaagctg attgaacacg gtatgcctgg cgaaatgccg
    28021 gtggcaattg tcgaaaacgg aacagcagtc acgcagcgcg tgattgacgg tacgctcacg
    28081 caactcggtg aacttgctca gcaaatgaac agtccatcgc taattattat tggtcgggtt
    28141 gttggcctgc gcgataaact gaactggttc tccaaccatt aatttaaccc gggccagaga
    28201 attctggcct tcttaatgac tctttttata tattcagcaa ataaaacata gccccttaat
    28261 aaatattatc tgctaatggg ttatattcac cgttacagcg acttacaaaa ttaagtcatt
    28321 cgctcactga cgccaattaa gtattcctgt atcgttctta acgagcagtc tgcttatatt
    28381 catataatca atgaatatta attaatatat aatacatagg gaatgtaaat gaacaaattt
    28441 attaaagttg cactggtagg tgcagtactg gctacgttaa ctgcatgtac tggtcatatt
    28501 gaaaaccgtg ataagaactg ctcttacgac tacctgctgc acccggcaat ttctatttct
    28561 aaaatcattg gcggttgcgg tcctactgca cagtaagtct ctgacagaaa tcggctaaca
    28621 ccggtttaat tgcccgacgc cctctggatt ttccgagggc gtatttgttt ctatatcctt
    28681 cttgctcccc acccgtaaaa atattttcac tttaatttca atttgataac cacattcatt
    28741 agccacagcg cgatcctatg cgaaattttg tgatcttcct ccgcattaca taacatcata
    28801 tgttgttata ttcattatgc attgtcatgt taccttttaa atgactgcaa actctcccct
    28861 acaacgtgtt ggacaagaaa aaggtatcgc tatgggaagc caggaactcc aacgcaagct
    28921 cggattttgg gccgttcttg caatcgccgt cgggacaacc gtcggctccg gtatttttgt
    28981 atctgtgggt gaagtggcaa aagcggcggg cacgccgtgg ctgacggtgc tcgcatttgt
    29041 catcggcggg ttaattgtga tcccgcaaat gtgcgtctat gcggaactat caaccgctta
    29101 tccggaaaat ggcgcagatt atgtttatct gaaaaatgcc ggaagccgac cgctggcttt
    29161 cctctccggc tgggccagct tctgggccaa cgatgcgccg tcattgtcga ttatggcgct
    29221 ggcgattgtc agcaatcttg gctttttaac gcctatcgat ccgttgcccg gtaaatttat
    29281 cgccgccgga ttaattatcg cctttatgtt gctacacctg cgctccgttg aaggcggcgc
    29341 agcgtttcag acgctaatta ccatcgccaa aattatcccg ttcactatcg tcattggcct
    29401 tgggatcttc tggtttaaag cggagaattt tgccgcccct accaccactg cgattggcgc
    29461 aacgggcagc tttatggcgc tgctggcggg gatctctgcc accagttggt cgtataccgg
    29521 catggcctct atctgttata tgaccggcga aattaaaaac cccggaaaaa ccatgccacg
    29581 agcgctgatt ggttcctgtc tgctggttct ggtgctctac accctgctgg cgctggtgat
    29641 ttccggcctg atgcccttcg acaaactcgc caattctgaa acgccgattt ccgacgccct
    29701 gacctggatc cccgcactcg gcagcaccgc tgggatcttt gttgccatca cggcgatgat
    29761 cgtcattctt ggttcgcttt ccagctgcgt gatgtaccag ccgcggctgg aatacgcgat
    29821 ggcgaaagac aacctgttct ttaaatgctt cggccatgtg catccgaaat acaacacgcc
    29881 ggatgtctcc atcatcctgc aaggggcgct ggggatcttc ttcatcttcg tttccgatct
    29941 caccagcctg ctgggttatt tcaccctggt gatgtgtttc aaaaataccc tcaccttcgg
    30001 ctccatcatc tggtgtcgta aacgcgacga ttacaaaccg ctgtggcgta ctccggcttt
    30061 cgggctgatg accaccctcg ccattgcgtc aagcctcatt ctggtcgcct caacctttgt
    30121 ctgggcaccg attcccggcc ttatctgcgc cgtcatcgtt attgctactg gtctgcctgc
    30181 ttacgccttc tgggcgaagc gtagccgcca gctcaacgct ttgtcgtaat gttatctgga
    30241 gaaaataaaa tgttggatat tgataaaagc accgtggact ttctggtcac cgaaaatatg
    30301 gttcaggaag tggaaaaagt tctcagccat gacgttccgc tggtgcacgc catcgtggaa
    30361 gagatggtga agcgcgacat tgatcgtatt tatttcgttg cctgcggctc gccactcaac
    30421 gcggcgcaaa cggcgaaaca tctggcggat cgcttttccg atcttcaggt ctacgccatt
    30481 tccggctggg agttctgcga taacaccccg tatcgcctcg acgatcgttg cgcggtgatt
    30541 ggcgtttctg actacggtaa aacggaagag gtaatcaaag cgctggagct gggccgggcc
    30601 tgcggcgcac tcactgcggc gttcaccaaa cgcgcggaca gcccgattac ctcggcggcg
    30661 gaatttagca ttgattatca ggccgactgt atctgggaaa ttcacctgct gctctgctac
    30721 agcgtggtgc tggagatgat cacccgcctc gcgccgaacg cggaaatcgg caagatcaaa
    30781 aacgatctca agcagttgcc gaatgcgctc ggtcatctgg tacgcacctg ggaagaaaaa
    30841 ggccgccagc ttggtgaact ggccagccag tggccgatga tttataccgt tgctgcgggt
    30901 ccgctgcgtc cgctgggtta caaagaaggc attgtaacgc tgatggaatt tacctggacg
    30961 cacggctgcg tgattgagag cggagagttc cgccatggcc cgctggagat tgtcgaaccg
    31021 ggcgttccgt tcctgttcct gctcggcaat gatgaaagtc gccacaccac cgaacgcgcc
    31081 attaactttg ttaaacagcg tactgacaac gtgatcgtca tcgattacgc cgaaatttcg
    31141 caagggctgc acccgtggct ggcaccgttc ctgatgttcg tgccaatgga gtggctctgc
    31201 tactacctgt ctatttacaa agatcacaac ccggatgaac gccgctatta cggtggtctg
    31261 gtggaatatt aatccctctc ccggcccggc aacgggccga atttacgcaa ggagttaccc
    31321 gatgaaaaca ggtatgttta cctgcggcca ccagcggctg cctattgaac acgcatttcg
    31381 tgatgcaagc gagctgggtt acgacggcat cgaaatttgg ggcggtcgcc cgcacgcgtt
    31441 cgcgccggac ttaaaagcgg gcggcatcaa acaaatcaag gcgctggcgc agacgtatca
    31501 gatgccgatt atcggctata cgccagaaac caacggctat ccgtataaca tgatgctggg
    31561 cgatgaacat atgcgtcgcg aaagcctcga catgatcaag ctggcgatgg atatggcaaa
    31621 agagatgaac gcgggttata cgctgatttc cgcggcccac gcgggctatc tcacgccacc
    31681 taatgttatc tggggacggc tggcagagaa cctaagcgaa ctgtgtgagt acgcggaaaa
    31741 catcggcatg gatctgatcc tcgaaccgtt aacgccgtat gaatcgaacg tcgtgtgtaa
    31801 tgctaatgat gtgcttcatg cgctggcgct ggtgccttcg ccgcgcctgt tcagcatggt
    31861 cgacatttgc gcgccgtatg tccaggcgga accggtgatg agttatttcg acaaactggg
    31921 tgataaattg cgtcatctgc atattgtcga cagcgacggg gccagtgaca cacattacat
    31981 tcccggcgaa ggcaaaatgc cgctgcggga gctgatgcgc gatattatcg accggggcta
    32041 tgacggttac tgcacggtgg aactggtgac gatgtatatg aacgagccca gactctatgc
    32101 ctgccaggcg ctggaacgct ttcgcgcgct gctgccggag gatgagagat ggaaaccctg
    32161 gcgacaatcg gcgataactg cgtcgatatc tacccgcaac tgaataaagc gttttctggc
    32221 ggtaatgcgg tcaatgtggc ggtgtactgc actcgctacg gcatacagcc gggatgcatt
    32281 acctgggtgg gtgacgatga ctacggcaca aagctgaagc aggatctcgc ccgcatgggc
    32341 gtcgatatca gccatgtcca tacgaaacac ggcgttaccg cacaaactca ggtggaactg
    32401 cacgacaatg atcgcgtttt tggcgactac accgaaggcg tgatggccga ctttgccctg
    32461 agtgaagagg attacgcctg gctggcgcag tatgacattg tgcacgcggc aatctgggga
    32521 catgcggaag acgcattccc acagctgcac gctgcgggca aacttaccgc tttcgacttc
    32581 tccgacaagt gggacagccc gctctggcag acactggtgc cgcatctcga ttttgccttt
    32641 gcctccgcac cgcaagaaga cgaaacgctg cgtctgaaga tgaaagcgat tgttgcccgt
    32701 ggcgcaggaa cagtgattgt cacgctgggt gaaaacggca gcattgcctg ggatggcgcg
    32761 cagttctggc gtcaggctcc tgaaccggtg acggttatcg acaccatggg tgccggagat
    32821 tcgttcattg ccggattcct ttgcggctgg tctgcgggga tgacattacc gcaggcgatg
    32881 gcgcagggaa cggcgtgcgc ggcgaaaacc attcagtacc acggtgcctg gtaggtataa
    32941 cgttggcgtg agcatcttca cgccaacgtg ctgttacttg ccggaaaacg accctataat
    33001 ccgagtaatt cattctttat ttcagggtcg attatgtcag ctacggaccg ctactctcat
    33061 caactcctct acgctaccgt ccgccagcga ctgctggatg atatcgcgca gggggtttac
    33121 caggccgggc aacagatccc taccgaaaac gagctttgta cacaatataa cgtcagccgc
    33181 attaccattc gcaaagccat cagcgactta gtggcagacg gcgtactgat ccgctggcag
    33241 ggaaaaggca cctttgtaca aagccagaaa gttgaaaacg ccctgcttac tgtcagtggt
    33301 tttaccgatt ttggcgtctc acaaggcaag gcgacgaaag agaaagtgat cgaacaggaa
    33361 cgggtcagcg ccgcgccgtt ttgcgaaaag ctgaacatcc ccggaaacag cgaagtgttc
    33421 catctctgcc gggtgatgta tctcgataaa gagccgctgt ttattgatag ttcatggatc
    33481 ccgctgtcgc gttatcctga ctttgatgag atttacgtcg aaggaagctc cacctatcag
    33541 ttatttcagg agcgttttga cacgcgagtg gtcagcgaca aaaagaccat cgatatcttt
    33601 gccgccaccc gcccgcaggc aaaatggctg aaatgcgaac tgggcgaacc gttgtttcgc
    33661 atcagcaaaa tcgcctttga tcagaatgac aaaccggtgc acgtctccga actcttctgc
    33721 cgcgccaatc gcatcacctt aactattgat aataaaagac attaaccgta ggccggataa
    33781 gatgcgccag catcgcatcc ggcgatgctg gcgcgttgaa ttttacatcc cgtacgttcc
    33841 cctcacccta accctctccc caaaggggcg aggggaccgt tcagtacgga atttagcttg
    33901 acggtgctat ccagcattaa atactggcaa tactctcacg caaaatccgc agcaccgtct
    33961 cttcaccgct gcgattcggg ttaatgcgaa tcgcacaatg ttctgattgt ggattcgcct
    34021 ggcgaaacgt tccggaaagg cgataaaaga gcggcgggat ttcatatttc gactctgcac
    34081 ccaccgggta aggcaaggca ccgcgctttt gcgcctcttc cagcactctg gcggcaatcg
    34141 gctgatgaaa ctcgacaatc aacaccttcg actgcgcatt agcaatcacc gcgcttttca
    34201 cttccggcac cgcaccaccg ttaagcaaag ccagcaaccg ttcagatacc cctgcctgca
    34261 ccgcgtgcat cactggcgca aacaccagac cacgcaatac ttccagcgcc tgtgcgccct
    34321 ggatctggct accgccggag taaagcgtgg cgcgaatacg gttgataaca tcagcatcgc
    34381 cgaccactgc accaacaccc tctggcccaa atagcttgaa gcaggaaaat gtcgagacat
    34441 tcgcgccgca ttcacagccg attcgcgcca ccttcatcac cgcatagttg tcatcggtta
    34501 acgctggaac acctgccgcg cgcaacgttg ccagcacatc tgccagcacg tagctgtcct
    34561 gcggctgctg gcgcgtatgc tgcaccagcg ccgcatccgg ttgttgctcg tcgacgacct
    34621 gcttcagtgc cgacaggtca ttgaaatcaa cagtaataag cgtcagcccc atctgctcaa
    34681 taataacccg tgtcgtcggg taaacaggcg cgtcatgcac cagaagacgc tgccccggtt
    34741 tgagcaaagc cgccagcccg gcgcgaatcg cgccagtccc cgcgccctgc accagcgccg
    34801 cagcctgtgc gtgaaatgca tcagccagca cctgttccac acgctgggta acgcgcggtt
    34861 gattcagtcc tggcgttaag cctaaatcac cgccagtaag aaactcgctg ccggggaaat
    34921 ggcgacaaat gctatccacc agcgcaaact gcttttgctg cgcctcaata atcgtcaggc
    34981 tttgcagagg aaacgtcttc ataggtgcct tccttaagcc ggaacgaaca ggccaagcca
    35041 gtaaagaaca ttcagcagaa taccggtgat cattactgcc acgaccggtg ccgccatttt
    35101 ctgtaccgga cggcccaggg attcgttgag gaagtaaatg gcaaccgcga tagagaatcc
    35161 ggtataaccc gccatcttaa ttgctgcgaa aatcgaaccg accagcagcg ccacttccat
    35221 cagcatattc atggcgttac ggatgttatc cgacgcatta cgcaccgacg gatagcgccc
    35281 cagccatttg ccgatcgaac gaagcaataa aacttctgcc gaaataacca ctgcacctaa
    35341 taccgctgca accatcggat tcggcgagag atagcccacc gcataaacaa aggtaaagcc
    35401 cgcaactgca tacacacccg ttgctaacgc ggtggtggca atcaacggta caaaacccag
    35461 cccgcgcata aattctgcca gtgccgcctg gttgatcagc gtttgcgatt gttccggcgt
    35521 tacgcctgcg gagtacgctt tctccagagt gaagatcgac acttcactgc cggcgaaaat
    35581 cttcatgctg gcaacggcgg caatcaatgc tcccacgatg gcgatatagg gtaagttttt
    35641 gataatccgc gacgtgcgtt cttcaaacac cgaaagcccg ctggcatcaa tatcattttc
    35701 atcacgatga cgcaggtcgt gagttatcgc gatccccagc agcatcacca tgccaataaa
    35761 gatttcgatg gattcagggt taagatgtgg gaaatagcgc acgactacca cacgggtcat
    35821 cagaacaacc acggcggcga ccagactttg cttccagcca aactggtaga aaatcgccac
    35881 cagcgggaac agtgcaaaag cagaaaccac cggcgagctt aattccccga ggctacctaa
    35941 tacatccacc ggcagcgcgg tcagcagctg gtttactggc aacaggcaag taaggatcaa
    36001 cacgccccag atagcgccaa gaccaaacgc catcaggctg tttatcgcca gtacgccgag
    36061 gatatcggta ggaagaaaga gtaaccatgc gttgagcagc ccggttttca gggtaaaaga
    36121 gatccccacc gaggccacaa aaccaatgct cagaccaaaa gcgatactcc ccgcctcgcg
    36181 acgattcata tagccttcaa tcagttgcgg caggatcggg cggatgccgt catgaaaaac
    36241 agccgccgag cgatgcgcca gaagcgatgt catacccgtc aggcacgcca ccacgataat
    36301 ctgaatatac agatccatag tcgtgccttt tatttcagat ggttaatcag catcggaatc
    36361 gcgtgttcga cgtgctcaac ggaaaggcca aacgccactt tcccttcagc gatcattttg
    36421 gcgatatgct cgtctttcgc tttaatgcct ggtttggcaa tggtgcaact tttgttatag
    36481 ccaatcaccg cgatggcaat cgacaatgcc gcgcccgcac cggtattaca cgcaccgatg
    36541 tagtaatcca gttgcccaga tttcactttc attgccgctt ccatgtcgtt gtgaatgaaa
    36601 acttcaaaac tgccaggagc cgtcgcttca atggtttttt taatctgctc acgctgtaag
    36661 cctgcaacgc caatcttttt catgagtctg tccttattgg aaaaattgag agggattttc
    36721 acgtaacatc acatccacat cggcctgact gaatcctgac tggcgcaatt gcggaataaa
    36781 ggtggtcaat aaatagtcat agccataacc accattggct tttaaatggg agcggcgcgt
    36841 aatatccatc gacagcatga cgcggttcag caacccacgg tcacgtagcg cgtggagcat
    36901 cgcaatgcgc ttttcgtccg ggtagtaact gttcttacca atggtgtcga actgcacgta
    36961 cgcgccgaga tcgatcatct tcaaaatgtt gtcgagattg tctttcagat cgcagtgacc
    37021 aacggtgacg cgcgaaagat caactccgtg ggcttgcaac agagccagtt gttccagccc
    37081 catggtgctg aacgaagtat gcgtggagat cgggcgtccg gtctggttat gcgccagcgc
    37141 agcggcaata aacaccttct cttccagcgg cgtaatcttc ccttcgctag agccgatctc
    37201 cgcgataatc ccggctttca ggtcggtgcc atcgataccc tgttcaattt catcgaccat
    37261 ctcctgcgcc agttcctgca cgctgcgggt cgccacatgt tccgggaaaa acgcgtcctg
    37321 gtaataaccg gtacaggcca ccacgttgat ccccgtctcg cgcattacat caagcataaa
    37381 ttgcgcattg cgccccatgt aacggttggt catctcaatc acattacgca cgccccgggt
    37441 catcaggtcg ttcatctcct ggcaaatgaa cgcatactga tcaaggcggc agtccacgtt
    37501 gtttttaaag ccggagagat caatatgcag atgctcatgg gcgagggtgt aacccgtcgg
    37561 atcaaaactc atagggtgtc tcctgcaaac cggagggagg aaagaaaaga gcgaccattt
    37621 tgtggcggtg gcgcgcggaa aaattcacac acggtagccc ccacatcaga aagcgtggtg
    37681 cgcacaccga gctgcgtagc gatcatccct tgctgataaa ccagcactgg caccacttcg
    37741 cgggtatggt ggctgtgacc aatggtcgga tcgttaccgt gatccgccat cacgaccagg
    37801 caatcatctg gctgcatcgc ctcaacaagc cgggcaaggt tacggtcaac gacctgcaaa
    37861 cgttcggcat aacgtgcgac gtcttctgca tgaccagcga ggtcggtttc ctggatattg
    37921 atgcaaataa acgccgtcgg atgcgtgtta aattcgtcga gggtgatatc cataatccgc
    37981 tggctatcca ccagattttg ccagctcacg ccataaggat tgttgacgat atctgccacc
    38041 ttacccacca gcacggttgg cacgcctgct tcatacagtt tttgtggcac ctgcactttt
    38101 tcatcgacgc catagcccat atgcacgacc tggaaaccgt tgtcataagc gccagaacgc
    38161 ggcgcattga taccaataaa gcgcccttct ttgctttctg cggcatcgag aatgcgttga
    38221 ctgtcggtta acaggccacc aaatgtaatg acccgaccga cctgtacctg ctcacgcacg
    38281 atacgaccaa ttttgattgc gtcgtcaaaa gagatcacag agagattggc ggtaatgtta
    38341 tagacctggc ctaaatccgc ctcgagatta tcgccaatcg caaccgcctg attgaccaac
    38401 agaaattgca gttcatcgcc acggcgctcc acctgccaac cggcggatac taatgcctgc
    38461 tcaacacggt caatcacatc gcaaaaaggc atccgcagcg gcggtaacgg gcgcgtgcct
    38521 aaaatttcct gatgccccat aaaggtatcg ccaccttcat gttgcagctc tgccacgccc
    38581 caggttgcgg aatctgacgg ctgcatatcg cctggcgcat aacccaatgc gttgattagc
    38641 cccagcttct ccagcgttgg tagctgcaaa tgcggcaact ggctcaggat gtgaccacag
    38701 gtattcgctc ccgcatcttg cggacgcacc agcgtgacat ctttcattgc ccctacgcca
    38761 aagctatcaa tcactaacac cacaaatcgc gccattacgc ccccagagag tttccaagac
    38821 tgtcataacg accaacgatt ttcggttcgc cacggtgaat accggacacc agcaccacat
    38881 cgctacgggt gacaaaaatc tgcgtgcgga aacagagcac cactgcactg ctcaccggat
    38941 actcgcctgc cagcggcagg gtgtagtcga tactgctgtc atccacagtt ttgagattgg
    39001 tttcagtaat cttttgattt tctggcgtaa acaccagcgc atgttgcgca tgaccacgac
    39061 ggtagtaacc gccgccgtag cagtagctgt cgccacggaa atgatgggag atttcactta
    39121 accagagcat cgcgatacgt tcaggctgat cgccctgctg gtttgccgga atagtgcccg
    39181 tcagcgcatg accgggttcg gcatgagtca caccgtattc cgccagcaat ggcagcgaag
    39241 tgcagctggt cgctgaaggc gcgttcagtt gctcaagtgc aataccagat ttcgccagtt
    39301 gatcccgtgc ctgtatcagc gtgtgaagat tcggtgtcgg caaaactttt ccggcagcct
    39361 catcccaaag caggcaaggg aaatgggtaa gtccggctaa atgcagcccc ggcagtttct
    39421 taatttcagc aaccacctcg tgcagcgaat gctgtacaaa accgctctcc tgccccggat
    39481 aaagaaaatc atcgtcgcta taaactttga gcagcacaaa ctgcacacgt ccggccttca
    39541 ccgctgccgc agaaacttcc cgcgctttgt cgagagtaaa cacggtgatg acgtcggtgc
    39601 cctgttcaac ggcgtcagca acctgatgac aagggatttg taccagatgc ccctgatgcg
    39661 ccaccgacaa accagcgcgg cgcatgactc gcgcctcttt gtaatccacc gccacaatgc
    39721 cgctgtaacc taatgccagc aatttttccg ccagccatgg gttacggcca aactgcttgg
    39781 tcatcagata cagttcaatg ccgtaaagcc gcgccgtctc aatcagtcgc ttaccgtttt
    39841 ccagtacctg atccacgtcg atcacccagc tgtccggggc gatcttgccc tgctgccaca
    39901 aacttagtgc ggcggaaatc agcgccgggt tctggcgttt caatgcttct acaaacatct
    39961 tgttgcctcg tttctgttcc ttgaattaaa tattcatttt tttgaatatt taggttgtgc
    40021 gacgtggatt tgtcgggagc ttcaaacctc ttcgttagca gccatccata atccataaag
    40081 gttggccagc aaatagcctt cttcacacgg gttaacttcc agtgcgaatt ccttcaacaa
    40141 cacctgatgc aattgcacaa cggcttgcca gtggctggat tgcgccagct ctgccagcaa
    40201 ttcgttatcc agcggctcta tttcttcacc acggcgactg cgcatcagtg cactcgccat
    40261 atgtgtcatc gccatcgttc cttgctcact gcgcaccggc agatggcact ctgtttccag
    40321 tacgttgacg acctgcatca tgcctttgca gatgtcctta tcaataacgc ctgcctcgca
    40381 aagcaggttg agtctggttt ccatatcaga tgcctttcgt ttaataactc ggttcctgtt
    40441 ccccactcac tacccgttgt tgatgggcga gcagggcgtg tttatctaca acggcacgca
    40501 gcagttgttg cattgggtaa tcatcgactc gcgtcaggac cacggcttcg gtggcctgta
    40561 aaaatcgcgg atcgtctgtg agcggcgtcg cctctaatcc cagcatggtc agttcgtttt
    40621 ccgccaccac gttccagatc accgcatcga catcgccttt aacaatgcgt tgtaaactct
    40681 cgtgataaga gagatcgact cgttccacat cactaccgcc aaaaaaaaca tcggtcatga
    40741 ttttctgatc cgccgaacgg ttatccagcc ccacgcgctt cacgtttgcg gactcgcctt
    40801 tacggcaaat caactggtgc tcgccaacgt aggtgtgcgg ccccaactcc agcgcgaggc
    40861 ataacccttt ttgcgtgaga taactttccg ccgccagtcg ggaaaccacc gccatgtcat
    40921 acacgccgtt aagtaaacac tccacgcgaa tatccgcgcc gcgcatatgc gcatagtaaa
    40981 aaggaatgcc atcaaactga gctttcagcc cgctcgctaa accttcgtac aagcgggtat
    41041 agggcaaagg catcgcacat accacgttgt tgatatccac atgactcagc aatgctttgt
    41101 tatccatctc gaccagataa ctgccattgc gcccacggcg ttcaatccgt atcgctccgc
    41161 ttgattccag cgttttcaac gcggcctgcg tcaggccaac cgatgaacgg cattcagttg
    41221 ccagctcatc tatggttttc aacctattac cgcatttttc acccaacaaa taacgggcca
    41281 gcgtcgtgat aacgacgcct tcttttttga taaacgttcg acgcataata tattttcaat
    41341 aaaatgaatg tttatatctt cattaaaatg aagataagtg gcaactgtga gatgtggaaa
    41401 cggcgaggcg cttcacattt tttcggatta ttgcaaatga ggataaaaaa accgggtttc
    41461 cccggtttca gagtgatgac gaagtgattt attcgacaat gcgagcaaag ggtaattccc
    41521 actggcgctt gcctgctccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtc
    41581 cgagcgtttt gatattacgg cttcgccaca aaaccaatcg cttcgtacac cgctttcagc
    41641 gtacgggaag cgtgtacact ggctttttct gcgccgtctt tcatcacctg ttgcaggaag
    41701 gcttcatcgt tgcggaaacg gtgatagcgt tcctgcaatt cagtcagcat accggaaacg
    41761 gcatcagcca cttcaccttt cagatgacca tacatcttgc cttcgaactg tttttccagt
    41821 tctgggatgc tctggcccgt taccgcagaa aggatatcca gcaggttgga aacgcccgct
    41881 ttgttctgca catcgtagcg aactaccggc ggctcgtcgg agtcagtgac cgcgcgtttg
    41941 attttcttca ctaccgattt cggatcttcc agcaggccga taacgttatt gcggttatcg
    42001 tcagacttgg acatcttctt ggtcggctcc agcagcgaca ttacgcgcgc gccagatttc
    42061 ggaataaacg gctccggcac cttaaagatc tcgccataca gcgcgttgaa acgctgggcg
    42121 atatcgcgac tcagttccag gtgctgtttc tggtcttcac ccaccggtac cagattagtt
    42181 tgatacagca ggatgtccgc agccatcagc accggatagt caaacagacc agcgttgatg
    42241 ttctcggcat aacgcgcaga tttatcttta aactgggtca tgcggctcag ttcgccgaag
    42301 taggtatagc agttcagtgc ccagcctaac tgcgcatgtt ctggtacgtg ggactgaaca
    42361 aaaatggtgc ttttctcagg atcgatacca caagccagat acaaggccag cgtatccagc
    42421 gtcgctttac gcagcttctg tgcatcctgg cgcacggtga tcgcgtgttg gtcaacgata
    42481 cagtaaatgc aatggtagtc atcctgcatg tttacccact gacgcagcgc acccatgtag
    42541 ttaccaatgg tcaattcacc tgagggctgt gcgccactaa aaacgatggg cttagtcatt
    42601 tttcgattcc tgattttcgc tatgcggaag ccctaatgcg ggcagaaggt catttataga
    42661 ctgataaatt acatcaggct ggctgagatc gatagcctcg ccgtagttat atccgtaggt
    42721 taagccaacg gatgggcaac ctgccgcttt tgccgcctga atatcattgc gtgagtcgcc
    42781 gacaaacagc atctgttgtg gggcaattcc catccgctca gccaccagta acagcgggtc
    42841 cggatgaggt tttttgtttt gcacatcatc gccgccgata accacgctaa agtatttggc
    42901 aatatctaag gcttcgagca gcggcgcgac gaacggcgtc ggtttgttgg tgaccaggcc
    42961 tagcggcagg cctttagcct gcaacgcgcc caacgtatcg gcaacgtgcg ggaacaaaaa
    43021 cgtcccctct tcggcaacct cgccatagta gcgatcgaac agtttacgca gaatacgtac
    43081 ctgttcttct gccggaatgt cgtcatcaac gggcggttta cccattgttt tacgctgagt
    43141 cgcacgttcc tgacgcgccc aggtcaatgc gcgctccatc agaacatctg cgccgttacc
    43201 aatccaggta ataacgcgtt cttcacctgc gacgggcaac tccagcgcat acagcgccat
    43261 atctaccgca gcagcaagac cgggagcact gtcgaccagc gtaccatcaa gatcaaaagc
    43321 gacgccgcga atatcttcaa acttattcat gacttacctt tgccagttca ctgcgcattt
    43381 catcaatgac ttttttgtag tctggctggt cgaagattgc cgaaccggcg acgaacatat
    43441 ccgcgcccgc cgcagcgatt tcgccaatgt tgttcacctt cacgccaccg tctacttcca
    43501 gacgaatgtc aaagccagac tcgtcgatac gacgacgtac ttcgcgcagt ttatccagtg
    43561 tttgaggaat gaacgactga ccgccgaaac caggattgac ggacatcagc aggatcacat
    43621 ccagcttatc catcacgtaa tccagatagc tcagcggtgt cgccgggtta aataccagtc
    43681 ccgctttaca gccattttct ttaatcagtt gcagcgtgcg gtcaacatgc tcggaggctt
    43741 ctggatgaaa ggtaatgatg ctggcaccag cggcagcgaa atcaggcaca atgcgatcga
    43801 caggtttcac catcaggtgt acgtcgatag gggcggtaat gccgtagtta cgcaaggatt
    43861 tcagcaccat tggcccaatc gtcagattgg gaacatagtg gttatccatg acgtcaaaat
    43921 gcacgacatc agcgccagct gccagggctt ttgcggtatc ttcacccagg cgggcaaaat
    43981 cagccgacag aattgagggg gcaatcaaat actgtttcat ccgcttctcc ttgagaatta
    44041 ttttttcgcg ggtgaaacga ctcctggttt gtacaaagcc agcagttcgt ccaccttttt
    44101 acgtgtgccg ccgttgctgc ttatactgcg tcgaactttg acgacatgca attttgcgcg
    44161 ctgataccac tcacgcgtta acatcgtatc gtgattggag atcagcactg gaatatggcg
    44221 ctcaaccaga ccttcggcga tctccgccag atgcgcttgt tgttcaagcg taaaactgtt
    44281 tgtgtgatac gccgtaaagt tggcggtcgc agacagcggt gcataaggcg gatcgcaata
    44341 gacgacggat gcatcatctg cgcgcgccat gctatcggcg taagactcac aatagaaaaa
    44401 ggcattctgc gctttttcag cgaagtgata caactctgct tccgggaaat agggtttttt
    44461 gtagcggcca aacggcacgt taaactcacc gcgcagattg taacgacaca ggccgttgta
    44521 accgtagcgg ttcaaatata aaaacagtac cgcccgacgg aacggatcct ggcttttgtt
    44581 gaactcttcg cggaactgat agtagacctc ggcgcaattt gtttcgggaa caaacagctc
    44641 gcgtgcggcc tgtacgtact catcagtacg catcttcaca atgttataga gactgatcag
    44701 gtcgctattg atatcggcaa ggatataacg agaaaagtcg gtgttgagaa acaccgaccc
    44761 ggcacctaca aaaggctcaa ccagacattc gcccttgggc aaatgccgtt taatatcatc
    44821 aagcagggga tacttgcccc ctgcccactt caaaaaagcg cgatttttct tcatgctgac
    44881 taactaatta caccttctcc ggctgtggag aaagctccga cagcatccag cgcttcaggc
    44941 aactcccgca acgataaccc attgcgggag ataaccttga ttacttcaga tcggcctgta
    45001 cctgacgcag cggtttcgcc cacgggtttt tggcctgaac atctgctggc aatgtagata
    45061 ccgctttttt cgcctcttct ttcgaagcgt acacgccaga aaccaggaca taccacggct
    45121 gaccattacg cgtcgtttca tagacaacgt agtttttcag attctctttc ttcgcccaac
    45181 cgttcaggtt gtcgtagtta gaggaactgc tcagctgcag agtgtaatgg ctggacggtg
    45241 ccgatttcaa cgaaccaaca ttacctgcgg tcttccctcc agcggctggt gttgccgtgg
    45301 tttgcgccgg ggatgccgtc tgtactggag ccgtagtcgc cgtctctttt ggtgctggcg
    45361 cagaagtcgc agccggtgcc ttcgtgctcg ccacaggagc agctggttca gtacgcttcg
    45421 gcgtctgtgc taccggcttc ggttccgttt tcacggttgc ttgcggtttt ttcggttcaa
    45481 tcaccgcctg ctgacgagct ggacgcgtag tggccggacg ttcagcagtt tgcgttttcg
    45541 ccgtgtcacg cgatgcattg ccattgcgta ctggcgcgac agttgctggt tcagtcggca
    45601 atgtagaatt gaccgccaca ttgttcaact gttgctgatt ttgtggctgg gtcagcgcat
    45661 tgttcaggtc accctgcact tcaacacgtt gttgaccatc cgttgccgcc ggggtttgcc
    45721 cttgagtcgg cgtagaagag atcggtggca gagaaacatc ctgctgagta ttttccgcag
    45781 acgtggttcc cggcgctggc tgcaccccat tcgcctgatc ggtcgcattg cctgcaagat
    45841 caatactctt ctcgccagac gcggtttgat cgctggaaga ggtcgagggg gcttttagcg
    45901 cagaaccgat accgatgatc aacagcagta gaaccagaat gccgacgccc atcatcatat
    45961 actgacgaga agcgggttta ctggctgctt ttttgcgctt acgcggacga cgctctacgc
    46021 gctcttcatc cacggtttca tcttcggatt cgtcaatttc ttcttcgatt tccggttcct
    46081 cattgcgctc tttttgcgca cgagtcggac ggcgatcgtc agtgtcatca agttcaatat
    46141 catcaaaatt gatctgcggt tcgccacgtt cagtacgctc agaacgttca gaagattgac
    46201 gagaacgacc agtacgacga tcgctgggat cgggtttcag ctcgtcttct ggtttgaatt
    46261 catccattta acaccccact aaaaggttaa tgcttaccac gttgcaatta acctgaagct
    46321 aatagaccgc ttgataagcg gcctgacctt tcttgttgtt acgctgattg acaatcggca
    46381 atggcgttaa gaacaagctc gtgcgaaacg ccgctgcgaa cttcactctt accaattgcc
    46441 aacggaagaa ttaagcgtat ctctcccgca aggactttct tgtcacgcag catatgcggt
    46501 aaatacgcct gcgcggacat ttcgcgcggc ccattgaccg gtaacccagc ccgcttgagc
    46561 agggttataa tacgctgcgt ttcggcagaa ctaaactgcc cgagacgttc cgacgtccgc
    46621 gccgccatca ccatacccgc agcgaccgct tcaccatgta accaattgcc ataccccatt
    46681 tcagcttcaa tggcatgacc aaaggtgtgt cccagattca gtaaagcacg taacccggtt
    46741 tcgcgctcgt cggcggcgac aacttctgcc ttcagttcac aacaacggcg aatacagtac
    46801 gccattgccg gaccgtccag acgcaacaac gcatccagat tctcttccag ccagttaaaa
    46861 aacgcaccgt caagaataat gccgtatttg atgacttctg ccagccccga agctaactca
    46921 cgcgggggaa gcgttttcag acagtcgaga tccaccacca ctgaagcagg ctggtagaac
    46981 gcgccaatca tgtttttacc gaggggatgg ttgaccgcag ttttgccgcc aacggaggaa
    47041 tcgacctgcg acagtaacgt cgtcgggact tgaatgaaac gaacaccgcg ctgataactc
    47101 gccgccgcga agccggtcag atcgcccact acgccgccgc caagcgccac cagcgtagta
    47161 tcgcgaccat gcggcttttg taacaacgcc gtaaagacgg tatcgagtac agccaggctt
    47221 ttatactgct cgccgtcagg gaggataacg ctatcgacgt taacacccgc ctgttcaagt
    47281 acgccgcgga ccttatcgag atacagagga gccagggttt cgttggtgac caacatgacc
    47341 tgctcgcccg atttcagcgg taagaatgaa gctggttcat taaacaaacc agatgcgatg
    47401 gtaattgggt aactacgttc cccgagagta acgacaatcc tctccataac gcgacatcca
    47461 ccttaattac tgtacccgca gacgagtgta tataaagcca gaattagttg ctttccagca
    47521 tgtgaataat ctggtttgca accactttag cgctttgatc atcagtacga atggtcacgt
    47581 cggcaatctc ttcatacagc ggattgcgtt cattggccaa cgcttccaga acttcacgcg
    47641 gcggtgtttc aacgtgcagc aacgggcgtt ttttatcacg ctgcgtgcgt gcaagttgct
    47701 tttcgatggt cgtttcaaga taaacgacaa cgccacgagc ggaaagacgg ttacgcgttt
    47761 cacgggattt cacagagccg ccgccagtag ccagcacaat accctgtttc tcggtcaact
    47821 cattgatgac cttttcttcg cgatcgcgga agccttcttc gccttctaaa tcgaaaaccc
    47881 agcccacatc agctccggtt cgtttctcaa tctcttgatc ggaatcgtaa aattccatat
    47941 tgagttgttg agctaactgg cgcccaatag tgctttttcc ggcacccata ggcccaacca
    48001 gaaagatatt gcgtttctct gccatttttt cggtactact aagactattc gttaatgata
    48061 aacccgcttc gctcagagag cgccgcagga catgaactga aacctcataa gatattgcga
    48121 gagtcagact gaaaattatc tcaatactcc agcgggtttg gcaactgaat aaatcaccca
    48181 tccctctgca tatctggtcg ctgcaagcgc gctgccttgc taccaccgct ctggcgataa
    48241 atcaccgggt aagattagcg taaaaaagac agcaaaatgc cgcctgaatg ataaatcatc
    48301 atcatggaac gcaggcagcg aagagcacca actctcaaat cggtactcct tgtatgctaa
    48361 atacctgcgc gcgtcaaata gatgaaacac gttcagcata aaaacattac cgcttttacg
    48421 gctgtttact cactggaaac cagtcgtggc gtgataaaca ccactaactc gcgtcgttca
    48481 tcttcttttc cgtcatgacg aaataattgc ccgaaccagg gaatgtcgcc aagcaacggt
    48541 acgctatcct gacccgattt atttttacgg gtaaaaatgc cgcccagcgc caacgtttct
    48601 ccgcttttga cctcgacctg cgtttcgatc tcctgcttat caatcgccag cacttcgcca
    48661 tcggcctgct gtagcacctg ccccggaacg ttctggctga tgtgtaattt cagccggatg
    48721 cgaccttttt gtaacaccgt gggcgtgacc tccatcccca ggacggcctc tttaaattcc
    48781 accgacgtcg cgccactttc cccgctggaa acctgatatg gaatttcgct cccctgttta
    48841 atgctggcag gctgaagatg tgaggccagc agacgcggac tggcgataat atccagctgc
    48901 tgtttttgtt cgagcgcgga aagctcaaga tccagcaagc gtccgttgat gcgcccaatg
    48961 ttaaaaccga catgcgttgt cgccgtcgct acggagaggt cgctaccaag cgtggtgact
    49021 tgcccaacgc caccagcgtg ttgcgcatcg gccagcgtcc atttcacgcc taactcacgc
    49081 aaactttttt cattaatggt gacaatatgc gccgacagct caacctgccc gaccggcaga
    49141 tccatttgcg ctacccactg ttcaagcgcg cttaacgccg ttttgttatc tcgtagcaaa
    49201 aggcgattgg tgcgtttatc gacggtcata ctccctttgg cactcagtag cttctccccc
    49261 gctttcgcca gttctcccgc gtcggcgtat tgcagggtta tactgcgatt ttccagcggc
    49321 agatttgcct gcgcccgcgc ctgctccgcc tcctggcggg cgatattgtt attctgccag
    49381 gcaatggaat gcactgagag aatgttgtct tcctgccgcg ttatcagtcc ggcgcttttc
    49441 actacagttt gtagtgcctg cttccaggga acatctgtta gatgtaacga caccgtaccg
    49501 ctgacgtctg gcgacacgac caggttcaac ttctcctgtt cagccagcgc ctgcaacacc
    49561 tgagctaccg gaacgtcatc caccatcagc gtcacttttt gcggctttgc cgcctgtacg
    49621 ccgggtatca gcatcaacag tagtgcggct atccattgct tcatttgtat ctccttgccg
    49681 ttgccacaac cattgtggcg gttcgcagtt tgtcccggta cccagcgtta gtacgtctgg
    49741 cgtcagctgt aaaattgtcc agccgttttc cagcacatcg ttttgctgca cccgtcgcca
    49801 tttcttttgc ccgtctttta ttacaccgat gatgcgctcg cctcgcccta ccatcccctg
    49861 atagcgccac tggctaagtt cgctaatccg gcatagatct tccggcggtt taaaagggtc
    49921 acgcataccg gttaaaaggc acaatgcaat acctgccaac aaccagcgtt taaccctcat
    49981 gcggcgtctc cagttgtagc gtgaacaaaa gatcatcacc ttccacgctt aacgaaaaac
    50041 ggctcacgct gacgttgcgc tctgccagcc gtgtaaatgc cgacggcact gcttcccaca
    50101 gcgttttcaa cgccaactcg cctccctgcg cggatggatg ccaggaaacc agttgcgcgc
    50161 cggataactg aaaatccagt ggcgaaaagg gcagcgtttt ttcctcgctg aagggagtgg
    50221 tgtctaccag gcgatacagt gcggcccact gctggtgatg acttgcccgt agtcgaatta
    50281 atgcgtcgcg ctcttcatgg tgtgtcgatg atagaaaaat gagcgtaact aacatcagca
    50341 accagactgc ccagcaaagc tggcggaggc ggggtgatgt ggcgaaccac cagtcaaaga
    50401 acatgttcat cgctaacctt ccttgttaac tgatactcaa attgccagcg tccctgcgca
    50461 tcctgctgcg tggctccccg ctgattgaga tgaaaagacg catcctggcg gagtgacgtt
    50521 tctagtgcgt ttaacgcggt aatgcttgtt gtcagcccct tgatctccag cgttccctgc
    50581 tgccagctta tcgttgtcag ccaggcgtgc tcgggtaaaa gcgccgccag tgcttccagc
    50641 gcagattgcc agtcgcgggt aaattgtcgc tggcgctgcc gttgcgaacg ctgttcgcgt
    50701 aactgctgtt gctccagcaa acgtggcttc gttatctgta agctgcgggc gagttgttgt
    50761 tccgcctgaa gcaaaactgc gtctatgcgc gcttcggcgc tgcctgtcag acgcagtatt
    50821 agcgttatcc cgacggccag cagcagaggc gcaacgaaca tcagcaacca gaaacgcaga
    50881 aaagcggtcc ggcgttgctg tcgccagggc aaaaaattaa ttggcgggtt catcagtacg
    50941 ccttcccaag cgccagcccc agcgcgatgg caaagtctcc acctgacggc ggtagcggcg
    51001 gctgacgaac agaaacggcc tcccagggat caaatccccc ttcgccacat atcgcgacgc
    51061 tttcgggatc aacggataac gctgccgcca gctccttcgc gctagtcatc cccaccgcca
    51121 gtttgcgccc ccagcgatag cgtgtcgccc acagccactg ttcgttatca cgccaggcca
    51181 gacattgctg atgagaaggt aaaaaaggca ggaatcgctg taatgcactg gcatccgggg
    51241 tgatcgcact aacatgaaca cgcaaccttt ctgccagcgt aagcagcgtt gccagctctt
    51301 tgctttgcgc ggcagtcacg ttataagcgg ggctgagtga gtcttcgcta taatcgaagc
    51361 gcagggagtc cggatccata tccagctcgc gggccatcgt ccctgacagc caggccgttt
    51421 gctcccgctc accaagggac atcgacgggc gcggaaatga ccgctgtaat gtgcgactgg
    51481 cgggaaacgc caacataatg tgatgacgct gcggcagttc gcgactccaa ggtaacaacg
    51541 ttttagccag ctgctgcgca tcaacaatcc gcccatcttt gataatgtcg ttctccagcg
    51601 gcaaccgcca ccagcgttgc aaaaagcatt cttttgcgcc ccgtacgatc gcaaccgcta
    51661 ccgcttcttg ctgttgtaaa tgcaaaccaa tttgccagat cttaaatgcc attgtgatga
    51721 tctccttatc acccgtcact ctgacgggta tatcaatgcg tctggcttgc ctttatacta
    51781 ccgcgcgttt gtttataaac tgcccaaatg aaactaaatg ggaaatttcc agtgaagttc
    51841 gtaaagtatt ttttgatcct tgcagtctgt tgcattctgc tgggagcagg ctcgatttat
    51901 ggcctatacc gctacatcga gccacaactg ccggatgtgg cgacattaaa agatgttcgc
    51961 ctgcaaattc cgatgcagat ttacagcgcc gatggcgagc tgattgctca atacggtgag
    52021 aaacgtcgta ttccggttac gttggatcaa atcccaccgg agatggtgaa agcctttatc
    52081 gcgacggaag acagccgctt ctacgagcat cacggcgttg acccggtggg gatcttccgt
    52141 gcggcaagtg tggcgctgtt ctccggccac gcgtcacaag gggcgagtac cattacccag
    52201 cagctggcga gaaatttctt cctcagtcca gaacgcacgc tgatgcgtaa gattaaggaa
    52261 gtcttcctcg cgattcgcat tgaacagttg ctgacgaaag acgagagcct cgagctttat
    52321 ctgaacaaga tttacctcgg ttaccgcgcc tatggtgtcg gtgctgcggc acaagtctat
    52381 ttcggtaaaa cggtcgacca actgacgctg aacgaaatgg ctgtgatcgc cgggctgccg
    52441 aaagcgcctt ccacctttaa tccgctctac tcaatggatc gtgccgtcgc gcggcgtaac
    52501 gtcgtactgt cgcggatgct ggatgaaggg tatatcaccc aacaacagtt cgatcagaca
    52561 cgcactgagg cgattaacgc taactatcac gcgccggaga ttgctttctc tgcgccgtac
    52621 ctgagcgaaa tggtgcgcca ggagatgtat aaccgttatg gcgaaagtgc ctatgaagac
    52681 ggttatcgca tttacaccac catcacccgc aaagtgcagc aggccgcaca gcaggcggta
    52741 cgtaataacg tgctggacta cgacatgcgc cacggctatc gcggcccggc aaatgtgctg
    52801 tggaaagtgg gcgagtcggc gtgggataac aacaagatta ccgatacgct gaaggcgctg
    52861 ccaacctatg gtccgctgct gcctgccgca gtcaccagcg ccaatcctca gcaagcgacg
    52921 gcgatgctgg cggacgggtc gaccgtcgca ttgagtatgg aaggcgttcg ctgggcgcgt
    52981 ccttaccgtt cggatactca gcaaggaccg acgccgcgta aagtgaccga tgttctgcaa
    53041 acgggtcagc aaatctgggt tcgtcaggtt ggcgatgcat ggtggctggc acaagtgccg
    53101 gaagtgaact cggcgctggt gtcgatcaat ccgcaaaacg gtgccgttat ggcgctggtc
    53161 ggtggctttg atttcaatca gagcaagttt aaccgcgcca cccaggcact gcgtcaggtg
    53221 ggttccaaca tcaaaccgtt cctctacacc gcggcgatgg ataaaggtct gacgctggca
    53281 agtatgttga acgatgtgcc aatttctcgc tgggatgcag gtgccggttc tgactggcag
    53341 ccgaagaact caccaccgca gtatgctggt ccaattcgct tacgtcaggg gctgggtcag
    53401 tcgaaaaacg tggtgatggt acgcgcaatg cgggcgatgg gcgtcgacta cgctgcagaa
    53461 tatctgcaac gcttcggctt cccggcgcaa aacattgtcc acaccgaatc gctggcgctg
    53521 ggttcagcgt ccttcacccc aatgcaggtg gcgcgcggct acgcggtcat ggcgaacggt
    53581 ggcttcctgg tggacccgtg gtttatcagc aaaattgaaa acgatcaggg cggcgtgatt
    53641 ttcgaagcga aaccgaaagt agcctgcccg gaatgcgata ttccggtgat ttacggtgat
    53701 acgcagaaat cgaacgtgct ggaaaataac gatgttgaag atgtcgctat ctcccgcgag
    53761 cagcagaatg tttctgtacc aatgccgcag ctggagcagg caaatcaggc gttagtggcg
    53821 aagactggcg cgcaggagta cgcaccgcac gtcatcaaca ctccgctggc attcctgatt
    53881 aagagtgctt tgaacaccaa tatctttggt gaaccaggct ggcagggtac cggctggcgt
    53941 gcaggtcgtg atttgcagcg tcgcgatatc ggcgggaaaa ccggtaccac taacagttcg
    54001 aaagatgcgt ggttctcggg ttacggtccg ggcgttgtga cctcggtctg gattggcttt
    54061 gatgatcacc gtcgtaatct cggtcataca acggcttccg gagcgattaa agatcagatc
    54121 tcaggttacg aaggcggtgc caagagtgcc cagcctgcat gggacgctta tatgaaagcc
    54181 gttcttgaag gtgtgccgga gcagccgctg acgccgcctc cgggtattgt gacggtgaat
    54241 atcgatcgca gcaccgggca gttagctaat ggtggcaaca gccgcgaaga gtatttcatc
    54301 gaaggtacgc agccgacaca acaggcggtg cacgaggtgg gaacgaccat tatcgataat
    54361 ggtgaggcac aggaattgtt ctgattaaaa aggcgcttcg gcgccttttc agttggctga
    54421 caaagtgcat ttgtttatgc cggatgcggg tgaacgcgtt atccggctaa caaaatcgtg
    54481 aaaaatcaat atattgcagg aaacccataa gcctgataag cgttgtgcat caggcaaact
    54541 tcacgcattt acactcgccc ctgccctttc aaccattcgc gcacgaggaa cagcgcgctg
    54601 acattacgcg cttcgttgaa gtcagggtct tccagcaaat ccatcatatg cgccagcggc
    54661 cagcgcacct gtggtagcgg ctctggctca tcgccttcca gcgattccgg gtagagatct
    54721 tgcgcgacca cgatattcat tttgctggaa aagtaagacg gtgccatgct gagcttcttc
    54781 aaaaaagtca gatcgttcgc accaaatcca acctcttctt ttagctcgcg gttagcggct
    54841 tcgtagacgc tttcacccgg atcaattaat cctttcgaaa aacctaattc gtaggattca
    54901 gttcccactg cgtattcgcg gatcaggatc aggtgatcgt ccacaatcgg cacaatcatc
    54961 actgcttccc ggttggttgg acgcattcgt tcataaacac gccgcacgcc attgctgaac
    55021 tccagatcca cgctctcgac ggtaaacagt cgggaacggg ctacagtttc aacattcaga
    55081 atggtgggtt tttgtaatga tttgctcatc gtgggatcta tgctgtgaaa tcagcggtta
    55141 ttgtgcgata tcggacacgc tttcggcaat gtgaattgca tgttatttac atttatgtaa
    55201 cttaataaat aatcgtcctc aaatcaaatt aaaagtcaat aggttgaaat aactccagga
    55261 atttgctgat attccgcctt cagagggttt gctatgatca gcggttactg tgatgtgctt
    55321 aatgatgctc aagttaaact ccacgcttgc cgatagccaa ccgcagaatc atgtattgtg
    55381 tctggtgcga ctgaccacgc ctgacagact aagtaagatg gggaaagcat gagcaccatt
    55441 gtgatttttt tagctgcttt gctggcctgc tcactacttg cgggatggct gataaaagtg
    55501 cgatccagac ggcgtcagct gccctggacc aacgccttcg cggatgcgca aacgcgtaaa
    55561 ctcacacctg aagaacgtag cgccgttgaa aattatcttg agagcctgac gcaggtatta
    55621 caggtgcctg gcccaacggg agctagcgcg gcaccgatct ctctggcgct gaatgccgaa
    55681 agcaacaacg tcatgatgct gacacacgct atcacgcgtt acggcatctc taccgacgat
    55741 ccgaataaat ggcgttacta cctcgattcg gtagaagtcc acctgccccc tttctgggaa
    55801 caatacatca acgatgagaa taccgttgaa ctgatttata ccgattcgct gccgctggtt
    55861 atttcactca acggtcatac gctgcaggag tacatgcagg aaacgcgcgg ctatgccttg
    55921 caacctgttc cgtccacgca ggcgtcgatt cgcggggaag agagtgagca aatcgagcta
    55981 ctcaatattc gcaaagaaac gcatgaagaa tatgcgctga gtcgtccgcg cgggctgcgt
    56041 gaagcgttgc tgatcgtcgc ctccttcctg atgttctttt tctgcctgat taccccggat
    56101 gtatttgttc cgtggctggc aggcggcgca ttactgctgc tgggcgcagg tctgtggggg
    56161 ctattcgcgc ccccggcaaa atcctccctg cgagaaattc attgtctgcg cggtacaccc
    56221 cgtcgttggg gattgtttgg cgaaaacgat caggaacaga tcaacaatat ttcactcggt
    56281 attatcgacc tggtctatcc cgcacactgg cagccataca ttgctcagga tctcggtcaa
    56341 caaaccgata ttgatatcta tctcgaccgc cacgtagtgc atcaggggcg atatctttcg
    56401 ctgcatgatg aagtaaaaaa ctttccgtta cagcactggc tgcgcagtac gattatcgct
    56461 gcgggctcgc tgctggtgct gtttatgctg ttattctgga tcccgctgga tatgccgctg
    56521 aaattcactc tctcatggat gaaaggcgcg cagaccattg aagccaccag cgtaaaacaa
    56581 ctggctgacg ctggcgtgcg ggtaggcgat acattgcgta ttagcggtac gggaatgtgt
    56641 aatattcgca cttccggaac ctggagcgcg aaaaccaatt cacctttttt accgtttgac
    56701 tgctcgcaga tcatctggaa tgacgcccgc tcattaccat taccagaatc tgaactggtc
    56761 aacaaagcga cggcattgac tgaagcagtt aatcgccagc tgcaccctaa accggaagat
    56821 gaatctcgcg tcagtgcctc attacgttca gcgattcaaa aatccggcat ggtatttctt
    56881 gatgattttg gcgacattgt actgaagaca gcggatttat gttctgccaa agatgactgt
    56941 gtgcgactga aaaatgcgct ggtcaatctc ggcaacagta aagactggga cgcgctggta
    57001 aaacgcgcca acgccgggaa gctcgatggc gtgaatgtgt tattacgccc ggtgagtgcg
    57061 gaatcgctgg ataacctggt ggcaacctcc accgcgccgt ttatcacgca tgaaacggcg
    57121 cgagcggcac aatcactaaa cagtccggcc cccggcggat tcctgattgt cagcgacgaa
    57181 ggcagcgatt ttgttgatca gccctggcct tcggcatcac tttacgacta cccgccgcaa
    57241 gaacagtgga acgctttcca gaaactggca caaatgctga tgcatacgcc gtttaacgcc
    57301 gaaggtatcg tcacaaaaat cttcactgac gccaatggta cgcagcatat tggccttcat
    57361 ccgatcccgg atcgttccgg cctgtggcgc tatctcagca ccacattgct gctactgacg
    57421 atgctgggta gcgccattta caatggcgta caggcctggc gtcgttacca gcgtcatcgc
    57481 actcgcatga tggagattca ggcctattat gaaagctgcc tgaacccgca actgatcacc
    57541 ccttcagaaa gccttatcga ataacacgtt tgcgcggcag gttatgctac cctgtcgcgc
    57601 aaattgcttc actctggaga tttccctcat gcatatcaac attgcctggc aggacgtaga
    57661 taccgttctg ctggatatgg acggcacatt gctcgacctc gccttcgata actatttctg
    57721 gcaaaagctg gtgcctgaaa catggggcgc gaaaaacggg gttacgccac aggaagcgat
    57781 ggaatatatg cgccagcaat atcacgacgt acagcatacg ctaaactggt actgtcttga
    57841 ttactggagt gagcaactgg gtctggatat ctgtgcgatg accaccgaga tgggaccgcg
    57901 tgccgtactg cgtgaagata ccattccgtt tcttgaggca ctgaaagcca gcggtaagca
    57961 gcgaattttg ctcaccaatg cgcatccgca caacctggcg gtaaaacttg agcataccgg
    58021 tctggacgca caccttgatt tattactttc cacccacaca tttggttatc cgaaagagga
    58081 tcagcggtta tggcatgcgg tggctgaagc tacgggtctg aaagctgaaa gaacgctgtt
    58141 tattgatgac agcgaagcga ttctcgatgc tgccgcgcaa tttggtattc gttactgcct
    58201 cggcgtgact aatcctgatt ccgggattgc cgagaaacag tatcaacgcc atccgtcact
    58261 gaatgactac cgccgcctga tcccctcgct aatgtgaagg agacgccatg aaagaaaaac
    58321 ctgctgttga ggttcgactg gataaatggc tatgggctgc ccgtttttat aaaacccgcg
    58381 cgctggcccg tgaaatgatt gaaggcggta aggtgcatta caacgggcag cgcagcaagc
    58441 cgagcaaaat cgtcgagctg aatgccacgc tcactctgcg ccagggaaat gacgaacgca
    58501 cggtgattgt aaaggcgatt actgaacagc gtcgccccgc cagcgaggca gccttgctgt
    58561 atgaagagac tgcggaaagt gtagagaaac gcgaaaaaat ggcgctggca cgtaaactta
    58621 atgccttaac catgccgcac ccggaccgac gcccggacaa aaaagagcgc cgcgacctgt
    58681 tacgatttaa acacggcgac agtgaataac tgtcacctgc aagagagatg attatgccgc
    58741 aacatgacca attacatcgc tatctgtttg aaaactttgc cgtgcgcggc gaactggtaa
    58801 ccgtttcgga aaccctgcaa cagatccttg agaatcacga ttatccgcag cccgttaaaa
    58861 acgtgctggc agaactgctg gttgcgacca gcctgttaac cgctacgctg aagtttgatg
    58921 gtgatatcac cgtacagctg caaggcgacg gtccgatgaa tctggcggtt attaacggta
    58981 acaataacca gcaaatgcgc ggtgtggcgc gcgtgcaggg cgaaattcca gaaaatgccg
    59041 acctgaaaac gctggtcggc aatggttacg tggtgatcac cattaccccg agcgaaggcg
    59101 aacgctatca gggcgtggtt ggtctggaag gtgataccct ggcggcctgc ctggaagatt
    59161 actttatgcg ttctgaacag ctgccgacgc gcctgtttat tcgcaccggc gacgtagacg
    59221 gcaaaccggc tgcaggcggt atgttgttgc aggtaatgcc tgcgcaaaat gcccagcagg
    59281 acgactttga ccacctggcg acgctaaccg aaaccatcaa aaccgaagaa ctgctgacct
    59341 taccggcaaa cgaagtgttg tggcgtttgt atcacgaaga agaggtgacg gtttacgatc
    59401 cgcaggatgt ggagttcaaa tgcacctgct cgcgtgaacg ttgcgccgat gcgctgaaaa
    59461 cgctgcctga tgaagaagtt gatagcatcc tggcggaaga tggcgaaatt gacatgcatt
    59521 gtgattactg cggtaaccac tatctgttca atgcgatgga tattgctgaa atccgcaaca
    59581 acgcgtctcc ggcagatccg caagttcatt aatgagtatg tccggcagag agggctctgc
    59641 cggattttac gacgtaatgg cctgatgtgc tgcgcttacc aggcctgtga aataaatcgc
    59701 aaattattgt tggtcagata cagcgtcgta tccgttaaaa gagttgtcac ttacttaagt
    59761 ttatttccag cgtttcacca tctaccgtca gatcgcgcca cgctttcggc ctcgtcccca
    59821 gttttccatc cttcgcagct gtgagtaatt tttctttcgt tatttcgttc ggaagtaaac
    59881 tcatatcaag cgcgtcccat tcttttctgt ctgagtcaaa gccatagaca atgactctgt
    59941 catcattaaa agcaaacagg atccgctccg cctgaccatc actattcaga tcttgctcga
    60001 ccaggacgca ggcgtctttt tcaatacagg tcatcacgtt atagcgatcc tggattaagg
    60061 ccgaccagaa tgtcgcatcc ggtttaacag aaccaggggc aattaacaca ttttcggcta
    60121 atactttttc cgatacctgt tcctgaagat gttgctctcc atctaacgcc atcagcagat
    60181 cccgcgcgcg tttcgggtct ttcataaacc cggcatcgct tttcagcgac tcaagcgccg
    60241 cacgtccata gcgaccgctc tgttcgagca tgtagatagt tacctggtct ggcgtgtttt
    60301 tgccgctctg ataacgcgcc atatgactgt tcacgctaat acgcatactg tccagcaccg
    60361 gcgaattaag aagcaccagt atcaccaaca ccaataacga aaccgcaagg ttcactttgc
    60421 cctgaagaac aaggggattt tgcccattac gccagacgat actgacaaaa taccccagcg
    60481 accacaccag taacaccagc accgccagcg cgccctgcaa gcggtcgaca gtccagccgt
    60541 actgagcgac ccgcagccat aacgcccagg cggcgacgaa cacatacagc ggagcaacca
    60601 gcaaagcggt tttaatcagg caacgcaacg gccccgtcca gggaagtgac gctttttgcg
    60661 gatcgcggac aatagccatt aagatcaatt gcaaaaaggc cagcgttaac agcaacccgg
    60721 cggcggagat gtggcgagaa atcgcgctca ggcccgtaaa cggcagagtg atgataaaca
    60781 tcagggttag caatgatact aacggcagca accccgtggc gattaacgtg aacaactttt
    60841 gaatagagtc gattaaacgt gactgtgtgc gcgcgagaat caccgccagt gcggtaacca
    60901 gacctaatgt aagatacata aaccagtcgg ttgcaaaaaa gagcgtattg aaaaacttga
    60961 tgccaacaag tttaaacaac tcaccccaca gtaaaagcac cagccacgtc aggccattgg
    61021 cgagaaaaat caccagtaat ataagtacgt tatgccatac tgactggtaa aagtagctat
    61081 agcggctact gccattgcag atacgaaggc tttgttgtat ccacggtagc aacaacattg
    61141 ccatcaacag cagatagcag ccaaaatccc aaatcgcctt ttcagctctc caggggttca
    61201 tgccatcagt ttgccacttc agccaaccgc tcatccctgt cgtggcaata aacaccagcg
    61261 ccagccatcc ccaaaggcgc ttctgtttaa aagagatcac ggagaagagt aaaacggatg
    61321 aaaacgcaac ggtcgcaggc acgccataaa caatccaact gtgatttttt ccggcaagcc
    61381 aggcgatcaa caggtagcaa accagcccct gaagtaatcc ggtagcaatc atcccccagc
    61441 gtgttgccgg tgaaagttca acgttatcca tgttggttat ccagaatcaa aaggtgggtt
    61501 aattatcgca tccgggcagt agtattttgc ttttttcaga aaataatcaa aaaaagttag
    61561 cgtggtgaat cgatacttta ccgattgaat ttgcatcaat ttcattaagg aatgcgattc
    61621 cactcacaat attcccgcca tataaaccaa gatttaacct tttgagaaca ttttccacac
    61681 ctaaaatgct atttctgcga taatagcaac cgtttcgtga caggaatcac ggagtttttt
    61741 gtcaaatatg aatttctcca gatacgtaaa tctatgagcc ttgtcgcggt taacaccccc
    61801 aaaaagactt tactattcag gcaatacata ttggctaagg agcagtgaaa tgcgcgttaa
    61861 caatggtttg accccgcaag aactcgaggc ttatggtatc agtgacgtac atgatatcgt
    61921 ttacaaccca agctacgacc tgctgtatca ggaagagctc gatccgagcc tgacaggtta
    61981 tgagcgcggg gtgttaacta atctgggtgc cgttgccgtc gataccggga tcttcaccgg
    62041 tcgttcacca aaagataagt atatcgtccg tgacgatacc actcgcgata ctttctggtg
    62101 ggcagacaaa ggcaaaggta agaacgacaa caaacctctc tctccggaaa cctggcagca
    62161 tctgaaaggc ctggtgacca ggcagctttc cggcaaacgt ctgttcgttg tcgacgcttt
    62221 ctgtggtgcg aacccggata ctcgtctttc cgtccgtttc atcaccgaag tggcctggca
    62281 ggcgcatttt gtcaaaaaca tgtttattcg cccgagcgat gaagagctgg caggtttcaa
    62341 accagacttt atcgttatga acggcgcgaa gtgcactaac ccgcagtgga aagaacaggg
    62401 tctgaactcc gaaaacttcg tggcgtttaa cctgaccgag cgcatgcagc tgattggcgg
    62461 cacctggtac ggcggcgaaa tgaagaaagg gatgttctcg atgatgaact acctgctgcc
    62521 gctgaaaggt atcgcttcta tgcactgctc cgccaacgtt ggtgagaaag gcgatgttgc
    62581 ggtgttcttc ggcctttccg gcaccggtaa aaccaccctt tccaccgacc cgaaacgtcg
    62641 cctgattggt gatgacgagc acggctggga cgatgacggc gtgtttaact tcgaaggcgg
    62701 ctgctacgca aaaactatca agctgtcgaa agaagcggaa cctgaaatct acaacgctat
    62761 ccgtcgtgat gcgttgctgg aaaacgtcac cgtgcgtgaa gatggcacta tcgactttga
    62821 tgatggttca aaaaccgaga acacccgcgt ttcttatccg atctatcaca tcgataacat
    62881 tgttaagccg gtttccaaag cgggccacgc gactaaggtt atcttcctga ctgctgatgc
    62941 tttcggcgtg ttgccgccgg tttctcgcct gactgccgat caaacccagt atcacttcct
    63001 ctctggcttc accgccaaac tggccggtac tgagcgtggc atcaccgaac cgacgccaac
    63061 cttctccgct tgcttcggcg cggcattcct gtcgctgcac ccgactcagt acgcagaagt
    63121 gctggtaaaa cgtatgcaag cggcaggtgc gcaggcgtat ctggttaaca ctggctggaa
    63181 cggcactggc aaacgtatct cgattaaaga tacccgcgct attatcgacg ccatcctcaa
    63241 cggttcactg gataacgcag aaactttcac tctgccgatg tttaacctgg cgatcccaac
    63301 cgaactgccg ggcgtagaca ctaagatcct tgatccgcgt aatacctacg cttccccgga
    63361 acagtggcag gaaaaagccg aaaccctggc gaaactgttt atcgacaact ttgataaata
    63421 caccgacacc cctgcgggtg ccgcgctggt agcggctggt ccgaagctgt gatgatttga
    63481 agctggataa tatctatcca gtatcttaga gaaagcaaaa cgggagacac cttcgcctcc
    63541 cgttttattt acccttcttt tgtcgtgccc tgcgcccgcg ttaccggcac tggcagccag
    63601 gcgcgaatgg aaagcccgcc ccgctcgctg gtgccaagct ccagcatccc gttatggtta
    63661 tccacgatac gctgcacaat cgccagccct aatcccgtgc cgctaatggt gcgcgcactg
    63721 tcgccgcgaa caaacggctg gaaaaggtgc ttacgctgtt ccggcgcaat tcccggaccg
    63781 tcatcttcca cctggaacca agcgcgattc ggctccgtcc cgctgctgac tttgatccag
    63841 ccattgccgt aacgggcggc attgaccacc atattcgcca ccgcgcgttt gatcgacagc
    63901 gggtgcattt tcacttcaat gctgccgggg taaagcgcgg tttcaatttc ccgctcatag
    63961 ccactttcgg cagcaataac ctcaccgagt actgcgttga gatccgccat ttccatcggc
    64021 atctcctgcc cggtgcgcag gtaatcgata aactgctcaa tgatggcgtt gcactcttcg
    64081 atatctttat tgatcgattc ggccaggtag ccatcctgtt cgctcatcat ctcggtcgcc
    64141 aggcgaatac gcgtcagcgg cgtgcgtaag tcatgactta cccccgccat cagcagcgtg
    64201 cggtcatccg ccagttgctt aacaccagcc gccatatggt taaaggcgcg ggtaaccgaa
    64261 cgcacctccg aagcgccata ctcacgcagc ggcggcggaa taatcccttt accgacctgc
    64321 aaagcagcat gttcgagatc gaccaacggt cggttctgga tacgaataaa cagccacgcc
    64381 ccgcctatcg ccaatagcat aatcgccaac gtatagcgga acagcgggga aaaatcgccc
    64441 tgatgaattt cggtcagcgg cacccgtacc cagatattgg gcgacagcca ggttttcagc
    64501 cagacgacag gcgaactttt gttgacctca acgcgcactt ccgtcgggcc gcccagttgc
    64561 tgtgccatct gatggcttaa gaattcatag tgttgtgccc aacgcagacc tgcctcttcg
    64621 gcagcttcgt tggagtagag agagatcccc agctcacggt agatctcccg acggaaagcg
    64681 ggaggcacaa ccaactgcgt gccgtcctcc agttgcagtt tgtcggtcat caacatacgc
    64741 acttcgtacg cgaggacttt attaaactgc tggaggctcg gcaaaatcgc gaagttcagc
    64801 accaccagat aagtcgtcac caggctggcg aacagcaagg tgacgatgag caataacgta
    64861 cgggcaaatg aacttcgtgg cgagaagcgc aatcgcctca tgctttagag ccgtccggta
    64921 caaagacgta gcccaggccc caaacggtct gaatgtaacg cggatgcgcc ggatcttctt
    64981 ccaccatgcg gcgcagacgt gaaatctgca cgtcgatgga gcgttccatt gcggaatatt
    65041 cacggccacg ggcaaggttc atcagcttat cgcgggagag cggctcacgc ggatggctga
    65101 ccagtgcctt cagtaccgca aactcaccgc tggtgagcgg catcggctca tcttcgcgga
    65161 acatttcgcg cgtaccgagg ttaagtttga acttaccgaa agcaattacc gcctcttcct
    65221 gtgacggtgc gcctggtagc tcgttcgcct gacgacgcag caccgcacgg atacgggcca
    65281 gcagttcacg cgggttaaac ggttttggaa tgtaatcgtc agcgccaatc tccaggccaa
    65341 cgatacggtc cacttcttcc cctttcgccg ttaccataat gatcggcatc gggttgctct
    65401 ggctacgaag acgtcggcaa atcgacaagc catcttcacc aggtaacatc aaatccagta
    65461 ccataagatg gaaagattca cgagtcagca ggcgatccat ctgttctgca ttagcgacgc
    65521 ttcgaacctg gaagccttgt tcggtgagat aacgttccag cagcgcacgc aggcgcatgt
    65581 cgtcatcgac caccagaatc ttgtagttct cttgcattgt ttgtactccc aaaggttcgc
    65641 aacaatttgt aagcgtgtat tcttaaaaaa gctcacgttc gtcaccagct aaatctggta
    65701 tgaatttcag cctaaattgt tacaaagcat attaaacagc agcttaagta tacaatttat
    65761 tcggcgaaac attattgatt ctgttgatat gatcacgtta tacccaatgt gcgcattatc
    65821 aaacagacaa agggaatcaa cgagatgaaa acgcccctgg ttacccggga agggtatgaa
    65881 aaactcaaac aagagcttaa ttatctctgg cgtgaagaac gcccggaggt cacaaaaaag
    65941 gtgacctggg ccgcaagtct gggcgaccgc agcgaaaatg ctgactatca gtataataaa
    66001 aagcgtctgc gtgaaatcga ccgtcgcgtg cgctatctca ctaaatgcat ggaaaatctc
    66061 aaaatcgtcg attactcccc tcagcaggaa ggcaaagtct tttttggcgc gtgggtggag
    66121 attgaaaacg acgatggcgt gactcaccgt ttccgtattg tcggctacga tgaaattttt
    66181 ggccgtaaag attacatctc tatcgattcc ccgatggccc gcgcattgct gaaaaaagaa
    66241 gtcggcgatc tggcggtggt gaatacccct gccggggaag cgagctggta tgttaatgct
    66301 atcgagtacg tgaaaccgta aggaagagtc ttaacctcct gccgatggct ggcatttttg
    66361 ccagccagtc cgtataacta tcccctgatt tttgatccga aaagatgaac tcaaaccatg
    66421 atgaatgatt cgttctgccg cattattgcg ggtgaaattc aggcgcgccc ggaacaggtt
    66481 gacgctgccg ttcgcctgct tgacgaaggg aataccgtgc cgtttatcgc acgttatcgt
    66541 aaggaaatca ccggcggtct ggatgacacg caactgcgta atctggaaac gcgtctgagc
    66601 tatctgcgcg agctggaaga gagacgtcag gcgatcctca aatccatttc cgagcaaggc
    66661 aaactcaccg atgatctggc gaaggccatc aacgccaccc taagcaaaac cgaactcgaa
    66721 gacctctacc tgccctacaa acctaaacgc cgcacccgcg ggcaaatcgc cattgaagca
    66781 gggcttgagc cgttggctga cctgctgtgg agcgatccgt cacacacgcc agaagtcgcc
    66841 gctgcacaat atgttgatgc cgataaaggc gtggcagata ccaaagccgc gctggatggc
    66901 gcgcgctata tcctgatgga acggtttgcc gaagatgccg tgctgctggc gaaagtgcgt
    66961 gattatctgt ggaagaacgc gcatttggtt tctacggtgg tgagcggtaa agaagaggaa
    67021 ggggcgaaat tccgcgacta tttcgatcat cacgaaccgt tgtccacggt gccttctcac
    67081 cgcgcgctgg cgatgttccg tgggcgtaac gaaggcgtac tccagctttc gctgaatgcc
    67141 gatccgcagt tcgatgagcc gcccaaagag agctattgcg agcaaatcat catggatcac
    67201 cttggcctgc gcctgaacaa tgccccggcg gatagctggc gcaaaggcgt agtgagctgg
    67261 acctggcgca tcaaggtgct gatgcatctg gaaaccgaac tgatgggtac cgtgcgcgaa
    67321 cgcgcggaag atgaagcaat caacgtcttt gcccgtaacc tgcacgatct gctgatggcg
    67381 gcccctgccg gactgcgtgc aacgatgggc ctcgatccgg gtctgcgtac tggggtaaaa
    67441 gtggcggtgg tcgatgccac tggcaaactg gtggcgaccg ataccattta cccgcacacc
    67501 ggacaagccg caaaagcagc gatgaccgtt gctgccttgt gtgaaaaaca taacgttgaa
    67561 ctggtggcga tcggcaacgg tacagcttcc cgcgaaactg aacgtttcta tctcgacgtg
    67621 cagaagcagt tcccgaaagt gaccgcacag aaagtgatcg tcagcgaagc tggcgcgtcg
    67681 gtttactcag cttccgagct ggcagcgcag gagttcccgg atctcgacgt ttcgctgcgt
    67741 ggtgcggtat ctatcgcccg ccgtttgcag gatccgctgg cggagctggt gaaaatcgat
    67801 ccgaaatcta tcggcgtagg tcagtatcag catgacgtca gccagacgca actggcccgc
    67861 aaactggacg cagtagtaga agactgcgta aacgccgttg gcgtcgatct caacactgct
    67921 tctgttccgc tgttaacccg cgtggcgggc ctgacgcgca tgatggcgca aaacatcgtg
    67981 gcctggcgcg atgagaacgg tcagttccag aaccgtcagc aactgctgaa agtgagccgt
    68041 ctggggccga aagccttcga gcagtgcgcg ggcttcttgc gcattaacca tggtgataac
    68101 ccgctggacg cgtctaccgt ccacccggaa gcctatccgg tggtggaacg cattctggca
    68161 gcaacacagc aggcactgaa agatctgatg ggtaacagca gcgaactgcg taacctgaaa
    68221 gcgtctgact ttactgatga aaaattcggt gtgccgacag taactgacat catcaaagag
    68281 ctggaaaaac cgggtcgcga tccgcgtccg gaatttaaaa ccgctcagtt tgccgatggc
    68341 gtcgagacaa tgaacgacct gcaaccgggt atgatcctcg aaggtgcggt gaccaacgtc
    68401 accaactttg gcgcgtttgt cgatatcggc gtgcatcagg acggcctggt tcacatctct
    68461 tcattgtcga acaagtttgt agaagatccg cataccgtgg tgaaagcggg cgacattgtg
    68521 aaggtgaaag tgctggaagt ggatcttcag cgtaaacgta tcgccctgac catgcgtctg
    68581 gacgagcagc ctggcgaaac caacgcccgt cgcggcggcg gtaatgaacg cccgcaaaac
    68641 aaccgcccgg cagccaaacc gcgcggacgt gaagcgcagc ctgccggtaa cagcgcgatg
    68701 atggacgcgc tggcggcagc aatgggcaaa aaacgttaaa cgccctgatt gacccctaaa
    68761 cctgcgcgat aaggtgtcca cgccgcatct ggcaacgaac gccttgtctg acataaaagt
    68821 gccggagaat atctccggca tttttattcc acagccaaac tcacaatata ttccggcaac
    68881 atttatcact tcattaacaa ctgaaacctt aattaaacat tagccagtcc tggtaattcg
    68941 ctattcgaat tatattttcg ctgcgatata accttgagcc acatcaacat tgagtcagat
    69001 tattattcaa accaacattc gcacacattt taagtattgc tgatagaaac cattctcatt
    69061 atcattgtgc tgttgattat ttaatctctc cttcgttggc aaatcatctg gtctcatgtc
    69121 gctgtcaaac gccccatgag gtagttatcc agttaatgag aaacaagtag gcacctatgc
    69181 aatacactcc agatactgcg tggaaaatca ctggcttttc ccatgaaatc agcccggcat
    69241 atcgccaaaa actgctttct cttggcatgt tacctggctc ctcttttaat gtggtgcgcg
    69301 tcgctccact cggcgacccc attcatatcg aaacccgtcg tgtgagcctg gtattacgca
    69361 aaaaagatct ggccttatta gaagtggaag cggtttcctg ttaatacggt gataacaaca
    69421 atgaaaaaat taaccatagg cttaattggt aatccaaatt ctggcaagac aaccttattt
    69481 aaccagctca ctggcgcacg tcagcgtgta ggtaactggg ctggcgttac cgtcgaacgt
    69541 aaagaagggc aattctccac caccgatcat caggtcacgc tggtggacct gcccggcacc
    69601 tattctctga ccaccatctc atcgcagacc tcgctcgatg agcaaatcgc ctgtcactac
    69661 attttgagtg gcgacgccga cctgctgatt aacgtggtgg atgcgtctaa ccttgagcgt
    69721 aacctgtacc tgacgctaca actgctggaa ctcggcattc cctgcatcgt ggcactgaac
    69781 atgctcgaca ttgccgagaa gcaaaatatt cgtattgaaa ttgatgctct gtcggcgcgt
    69841 cttggctgcc cagtgatccc gctggtttca acccgtggtc gcggtattga agcgctcaag
    69901 ctggcgattg atcgctataa agctaacgag aatgtggaac tggtgcatta cgcacagccg
    69961 ctactcaacg aagcagattc actggcaaaa gtgatgcctt ccgacatccc gctgaaacaa
    70021 cgtcgctggc tgggcctgca aatgctggaa ggcgatatct acagccgcgc ctacgccggt
    70081 gaagcgtcgc agcatctgga tgccgccctc gcccgtctgc gtaatgagat ggacgatccg
    70141 gcgctgcaca ttgccgatgc gcgttaccag tgcattgctg ccatctgtga tgtggtaagc
    70201 aacaccctga cggcagaacc cagccgtttc accactgcgg tagataaaat cgtgctcaac
    70261 cgtttcctcg gtctgccgat tttcctcttt gtgatgtacc tgatgttcct gctggctatc
    70321 aacatcggcg gggcgttaca gccgctgttt gacgtcggct ccgtggcgct atttgtgcat
    70381 ggtattcaat ggattggcta cacgctccac ttcccggact ggctgactat cttcctcgcc
    70441 cagggcctgg gtggcggtat taacaccgtg ctgccactgg tgccgcagat tggcatgatg
    70501 tacctgttcc tctccttcct tgaggactcc ggctatatgg cgcgtgcggc gtttgtgatg
    70561 gaccgcctga tgcaggcgct gggcttgcct gggaaatcct ttgtgccgct gatcgtcggt
    70621 ttcggttgta acgtaccgtc ggtaatgggc gcacgtacgc ttgatgcacc gcgtgaacgt
    70681 ctgatgacca tcatgatggc accgtttatg tcctgcggcg cgcgtctggc tatcttcgca
    70741 gtattcgcgg ctgccttctt cgggcagaac ggtgccctgg cggttttctc gctgtatatg
    70801 ctgggtatcg tgatggcggt gctgactggc ctgatgctca agtacaccat catgcgcggt
    70861 gaagcgacgc cgtttgtcat ggagctgccg gtctaccatg taccacacgt taaaagcctg
    70921 attatccaga cctggcagcg tctgaaaggc ttcgttctgc gtgctggtaa agtgattatc
    70981 atcgtcagca ttttcctgag cgccttcaac agcttctcgc tgagcggaaa aatcgtcgat
    71041 aacatcaacg actcggcgct ggcttccgtc agccgggtga tcaccccagt cttcaagccg
    71101 attggcgtgc atgaagataa ctggcaggca acggttggcc tgtttacagg tgcaatggcg
    71161 aaagaagtgg tggtgggcac gctcaacacc ctctacaccg cagaaaatat tcaggacgaa
    71221 gagttcaatc cggcagaatt taacctcggt gaagagctgt tcagcgcggt agatgaaacc
    71281 tggcagagcc tgaaagacac cttcagcctt agcgtactga tgaatcccat tgaagcgagc
    71341 aaaggcgacg gcgaaatggg taccggggcg atgggcgtga tggatcagaa attcggtagc
    71401 gcagctgccg catacagcta cctgattttc gtcctgctgt atgtaccatg tatctcggtg
    71461 atgggagcca tcgcccgtga atcaagccgt ggctggatgg gcttctccat cctgtggggg
    71521 ctgaatatcg cttactcact ggcaacattg ttctatcagg tcgccagcta cagtcagcac
    71581 ccaacttaca gcctggtgtg cattctggcg gtaatcctgt ttaacatcgt agttatcggt
    71641 ctgctgcgcc gcgcgcgtag ccgggtggat atcgaactgc tggcaacccg caagtcggta
    71701 agcagttgct gcgcagccag caccaccggt gattgccatt aatggcttca cttattcagg
    71761 tacgtgattt gctggcgtta cggggccgta tggaagcgac ccagataagc cagacattga
    71821 acactccgca gccaatgatt aacgccatgc tgcaacagct ggaaagcatg ggcaaagccg
    71881 tgcggattca ggaagaacct gacggctgcc tctctggcag ttgtaaaagc tgcccggaag
    71941 gcaaagcctg tctgcgcgag tggtgggcgc tgcgttaacc atactccatc gcctgttttg
    72001 aaaagcccgg tatgcgtttg catccgggct tttttgcgtg cggctttcca taaaaatgca
    72061 attcttgcag cgcgacgtaa gttcctctga aagcgtctcg cagggatgaa aactggctaa
    72121 tgcacagatg tggagtggcg cgtagagtcg cggcattcaa acaacaggtg aaggaacgcc
    72181 atgagtaaaa agcagagttc caccccacat gatgcgctgt tcaaactctt tttacgccaa
    72241 ccggagacgg cacgcgattt tcttgcgttt catttaccgg cacccattca cgcgctctgt
    72301 gatatgaaaa ccctcaagct ggagtcgagc agctttattg atgacgatct gcgtgaaagc
    72361 tattccgatg tgctgtggtc ggtgaaaacg gaacaaggac caggatacat ctattgtctg
    72421 attgaacatc aaagcacctc aaacaaactg atcgcatttc gcatgatgcg ttacgctatt
    72481 gccgcaatgc aaaatcacct tgatgctgga tacaaaacgt tgccgatggt ggtgccattg
    72541 ttgttttacc acggtattga aagcccctat ccctattcgc tgtgttggct ggattgtttc
    72601 gccgatccca aactggcaag gcaactttat gcctccgcat ttccgctgat tgatgtcacc
    72661 gtcatgcctg atgatgaaat catgcagcac cgacgtatgg cgctgctgga gttaattcaa
    72721 aaacatattc gtcaacgcga tctgatgggg ctggtagagc aaatggcctg cttattaagt
    72781 agtggatacg ctaatgacag acaaatcaaa gggctgttta attacatact gcaaactggc
    72841 gacgctgtac gttttaacga ttttatcgac ggcgttgccg aacgttcacc gaaacacaag
    72901 gagagtttaa tgactattgc ggaaagattg cggcaggagg gggaacaatc caaagccctg
    72961 catatagcca aaataatgct tgaatccgga gtccctctta cagacatcat gcgctttacc
    73021 ggggtgtcag aagaagagtt ggctgcggcg agccagtaaa tttctgtctc gccatttcaa
    73081 aagttgccta caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
    73141 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
    73201 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
    73261 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
    73321 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
    73381 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
    73441 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
    73501 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
    73561 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
    73621 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
    73681 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
    73741 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
    73801 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
    73861 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
    73921 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
    73981 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
    74041 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
    74101 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
    74161 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
    74221 ggttgcaatt gccggatcgc atcgtcagcg ttccgttatg gcagcggcgt cactggcgtc
    74281 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
    74341 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
    74401 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
    74461 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
    74521 tgcttttacg caatggtgcg gcggctgtcc aggtctggtg cctttgtcga accttgtaga
    74581 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
    74641 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
    74701 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggcgtt
    74761 tcttattgtc cgccggacgc tgtggaagcc accgacacag ccctgaaatt tgacctgctg
    74821 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
    74881 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
    74941 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
    75001 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
    75061 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
    75121 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
    75181 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
    75241 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcgcagc
    75301 attgctttgg caaatccctc ccttcgtttc tccgcgataa tatgaccaac ctctcataat
    75361 ttaaatttac cccgctctgg tgattctcaa acgccagatg ttacccgtat cattcacatg
    75421 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
    75481 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
    75541 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
    75601 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
    75661 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
    75721 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
    75781 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
    75841 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
    75901 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc ctctggtgtt taccatcgcg
    75961 gcttccgcga atattccact gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
    76021 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
    76081 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
    76141 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
    76201 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacttct
    76261 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
    76321 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
    76381 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
    76441 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
    76501 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
    76561 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
    76621 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
    76681 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
    76741 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
    76801 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
    76861 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
    76921 gcatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
    76981 agcaacttgt tcacgccatc atcggcaaac atcgtctcaa gcgttgcgga aagcttgcgt
    77041 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
    77101 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
    77161 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
    77221 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
    77281 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
    77341 tccccgctct cccagtaacc gcgtagcgta ggcaggtcat gtgtcgccgc aaccgccatc
    77401 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
    77461 agcactttgt aggagtacac accgctgctg cgcagcttac cgacaatctc taccggtacg
    77521 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
    77581 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
    77641 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
    77701 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
    77761 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
    77821 ccaaccgacg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
    77881 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
    77941 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
    78001 tcattacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt ctgatacatc
    78061 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
    78121 gcatcaaagg ctgcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
    78181 gcggtcatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
    78241 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
    78301 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
    78361 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
    78421 gggctggcac tctccggatt tgccggatag agcgcatgaa tcgggttcag gccgatgaac
    78481 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
    78541 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
    78601 ttattcagca acgcctgcgg ttcgtaacag cgtttcgggg cgacaatcac ccggcaatgc
    78661 gcgcgctggt cgtcctgggt gagtgtcagc gtgtgataac cttccggcag cttcgtcggt
    78721 agattgaacg ctttgccccc cgttacatgg cctttgtact gtgttccttc ttcggtggtc
    78781 agcagccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
    78841 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
    78901 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
    78961 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
    79021 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
    79081 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
    79141 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
    79201 accagatacg gatcgccgcc ttgtttgccg atactgtgca gcatctggtc gaaggcatgc
    79261 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
    79321 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
    79381 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
    79441 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
    79501 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
    79561 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
    79621 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
    79681 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
    79741 tgcgggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
    79801 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
    79861 ataccggtac gaactttcac aaactccgcc agacggactt tattcgcctg cttgatctcg
    79921 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
    79981 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
    80041 cactgtttga tccagcgacg tggggtaata ccgttggtga cgttatggaa tttgttcggc
    80101 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
    80161 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
    80221 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
    80281 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
    80341 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
    80401 aaagttttgc tggtaatggc ccaggcgtca tcccagctca tctggtgctc atcgatcagc
    80461 acgcgcagca gttctggaat cgcgatagtt gggtgggtat cgttcagctg aataacttcg
    80521 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
    80581 accgaacagg cacactggaa gtactgctgc atcaagcgca gctttttacc ggcagtatgg
    80641 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccctg ctgttcggca
    80701 cgcaggaaat caccgtcgtt aaatttagtc agatcaaacg gatgcgcgtg cgtcgcctgc
    80761 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
    80821 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcgtcacttt accgccaatc
    80881 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
    80941 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
    81001 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
    81061 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
    81121 tccgtcagat tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
    81181 ttcaacaggt tgttgcccgt caggcgacca atcaaaaact ccattgagat gtagttaaca
    81241 tgtcgctgat tcgccaccgg cttggcgaat ggctgagcac gcagcatttc ggccagtgct
    81301 tcactcactg ctagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
    81361 cgctgccact gacgtgaaag cgcttcctga aattgcttat cgttaaaaat aggttgtgac
    81421 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
    81481 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
    81541 gaaatgtgat ctcaaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
    81601 caatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
    81661 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcataa gtaaaatatc
    81721 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
    81781 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
    81841 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
    81901 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
    81961 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
    82021 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
    82081 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
    82141 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
    82201 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
    82261 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
    82321 ggcgcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    82381 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    82441 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    82501 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    82561 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    82621 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    82681 cggttgggcg acggcactac agctaatcgc cctctccgcc cggcagaata cccactcagc
    82741 ccataagtcg gcacgccgcc tagcgggaat caatgccagc catctttcgg attatctggt
    82801 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    82861 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    82921 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    82981 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    83041 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    83101 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    83161 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    83221 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    83281 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    83341 acatgaaatc aaggacatca gagaaggcac catgcacgca gaatttaacg ctctgcgcgc
    83401 ccaggtggcg attaacgatg gtaatccgga tgaagcggaa cggctggcaa aactggcact
    83461 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    83521 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctaatgcagc aaaccgaaca
    83581 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaatcc agcaaagtga
    83641 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acacaggaaa aagcgttcca
    83701 gctgatcaac gagcaacatc tggaacagct gccgatgcat gagtttctgg tgcgcatccg
    83761 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    83821 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    83881 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagctga accgtctgga
    83941 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    84001 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    84061 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    84121 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    84181 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    84241 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    84301 actggcgaat cgcaccggat ttatcagcca ttttgtcatc gaaggcgaag cgatggcgca
    84361 acaactgcgc cagctgattc aactcaatac gctgccggaa ctggaacagc atcgcgcaca
    84421 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    84481 tttcgttgaa cgtctgctga atcatccgga agtgccagaa ctgatccgca ccagcccgtt
    84541 gacccaacgt gaatggcagg tcctggggct gatttactcc ggttacagca atgagcaaat
    84601 agcgggtgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    84661 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    84721 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    84781 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    84841 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccttaa
    84901 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    84961 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    85021 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    85081 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    85141 catgcaggga aaaactcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    85201 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    85261 aggtgttaaa cgatgccacg ggcgagactt ccgttgccac cacgccgcct ccggcaggat
    85321 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    85381 ccagcacccg gcggataaaa tccgcaggcg gggccgacgg gttatcggtg cctccgctca
    85441 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    85501 gcgtacagct tccggcacta ccgatagcaa agcggtactc gccgccgcgc acggtgccag
    85561 gccggaagac cagacgctgc gaccccagct ccgcgccttc caccgttgcc ctacaaattt
    85621 ccgcagccgc ttttaccgcg gtcagatgct ggcgcaacag ccccggtttc gcccgcccgg
    85681 cacgaatgct ggtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    85741 gcaggatctg cccgccacct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    85801 tttatccttt tacgcacacc acctgacgca gggtatagat aacttccacc agatcgcttt
    85861 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    85921 catctttacg gcattccaca tgcgcggtgg cacgaatttg atcttccacg ctgaacagtt
    85981 ttttcgcttt agtacggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    86041 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    86101 catattgacc agcacgcgca gacaccgcgc ctttacgcgt cacgtagatc tcttcaccaa
    86161 agtgctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    86221 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccacg ttttccatca
    86281 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    86341 caaagtattc cgtaccttcc ataaagtacg ccaggtcgcg cgatggcaac gtttcaagct
    86401 ggtcctgcat ctctttttgt gccaaatcga taaagtaagt cccgatggcg ttaccaattc
    86461 cgcgtgaacc ggagtgcagc ataatccaca cctggtctga ctcatcaagg cagatttcaa
    86521 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    86581 gcggatattt ttgcgttaac cactgataac cggcttcaag ctcagcccat ttcgcatcga
    86641 cgttaacagg cgggttttcc catgcgcctt tatcacgttt acaacggcca gtggtacgcc
    86701 cgtgcggcac ggccatttca atcgcctgac gcagctctgc caggttttca ggcaggtctt
    86761 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    86821 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    86881 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    86941 gttgctgacg ctcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    87001 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgctgtcgt
    87061 gataattgc
//
LOCUS       DAZGAN020000005        84081 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGAN020000005 DAZGAN020000000
VERSION     DAZGAN020000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 84081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 84081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.005"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..100)
                     /gene="timP"
                     /locus_tag="ACHKYO_RS01965"
                     /old_locus_tag="pgaptmp_000393"
     CDS             complement(<1..100)
                     /gene="timP"
                     /locus_tag="ACHKYO_RS01965"
                     /old_locus_tag="pgaptmp_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HGU4427293.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLV"
     gene            complement(436..1281)
                     /gene="sseA"
                     /locus_tag="ACHKYO_RS01970"
                     /old_locus_tag="pgaptmp_000394"
     CDS             complement(436..1281)
                     /gene="sseA"
                     /locus_tag="ACHKYO_RS01970"
                     /old_locus_tag="pgaptmp_000394"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HGU4427294.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            1488..6449
                     /gene="yfhM"
                     /locus_tag="ACHKYO_RS01975"
                     /old_locus_tag="pgaptmp_000395"
     CDS             1488..6449
                     /gene="yfhM"
                     /locus_tag="ACHKYO_RS01975"
                     /old_locus_tag="pgaptmp_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HGU4427295.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGTAEDLLIVRP"
     gene            6450..8762
                     /gene="pbpC"
                     /locus_tag="ACHKYO_RS01980"
                     /old_locus_tag="pgaptmp_000396"
     CDS             6450..8762
                     /gene="pbpC"
                     /locus_tag="ACHKYO_RS01980"
                     /old_locus_tag="pgaptmp_000396"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HGU4427296.1"
                     /translation="MPRLLTKRGYWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            8911..9342
                     /gene="ndk"
                     /locus_tag="ACHKYO_RS01985"
                     /old_locus_tag="pgaptmp_000397"
     CDS             8911..9342
                     /gene="ndk"
                     /locus_tag="ACHKYO_RS01985"
                     /old_locus_tag="pgaptmp_000397"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGU4427297.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            9492..10646
                     /gene="rlmN"
                     /locus_tag="ACHKYO_RS01990"
                     /old_locus_tag="pgaptmp_000398"
     CDS             9492..10646
                     /gene="rlmN"
                     /locus_tag="ACHKYO_RS01990"
                     /old_locus_tag="pgaptmp_000398"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HGU4427298.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            10931..11944
                     /gene="rodZ"
                     /locus_tag="ACHKYO_RS01995"
                     /old_locus_tag="pgaptmp_000399"
     CDS             10931..11944
                     /gene="rodZ"
                     /locus_tag="ACHKYO_RS01995"
                     /old_locus_tag="pgaptmp_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGU4427299.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            11971..13089
                     /gene="ispG"
                     /locus_tag="ACHKYO_RS02000"
                     /old_locus_tag="pgaptmp_000400"
                     /gene_synonym="gcpE"
     CDS             11971..13089
                     /gene="ispG"
                     /locus_tag="ACHKYO_RS02000"
                     /old_locus_tag="pgaptmp_000400"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HGU4427300.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            13200..14474
                     /gene="hisS"
                     /locus_tag="ACHKYO_RS02005"
                     /old_locus_tag="pgaptmp_000401"
     CDS             13200..14474
                     /gene="hisS"
                     /locus_tag="ACHKYO_RS02005"
                     /old_locus_tag="pgaptmp_000401"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGU4427301.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            14492..15112
                     /gene="yfgM"
                     /locus_tag="ACHKYO_RS02010"
                     /old_locus_tag="pgaptmp_000402"
     CDS             14492..15112
                     /gene="yfgM"
                     /locus_tag="ACHKYO_RS02010"
                     /old_locus_tag="pgaptmp_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HGU4427302.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            15123..16301
                     /gene="bamB"
                     /locus_tag="ACHKYO_RS02015"
                     /old_locus_tag="pgaptmp_000403"
     CDS             15123..16301
                     /gene="bamB"
                     /locus_tag="ACHKYO_RS02015"
                     /old_locus_tag="pgaptmp_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HGU4427303.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            16419..17891
                     /gene="der"
                     /locus_tag="ACHKYO_RS02020"
                     /old_locus_tag="pgaptmp_000404"
     CDS             16419..17891
                     /gene="der"
                     /locus_tag="ACHKYO_RS02020"
                     /old_locus_tag="pgaptmp_000404"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGU4427304.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            17960..18175
                     /gene="yfgJ"
                     /locus_tag="ACHKYO_RS02025"
                     /old_locus_tag="pgaptmp_000405"
     CDS             17960..18175
                     /gene="yfgJ"
                     /locus_tag="ACHKYO_RS02025"
                     /old_locus_tag="pgaptmp_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU4427305.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(18172..19542)
                     /gene="xseA"
                     /locus_tag="ACHKYO_RS02030"
                     /old_locus_tag="pgaptmp_000406"
     CDS             complement(18172..19542)
                     /gene="xseA"
                     /locus_tag="ACHKYO_RS02030"
                     /old_locus_tag="pgaptmp_000406"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGU4427306.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            19704..21170
                     /gene="guaB"
                     /locus_tag="ACHKYO_RS02035"
                     /old_locus_tag="pgaptmp_000407"
     CDS             19704..21170
                     /gene="guaB"
                     /locus_tag="ACHKYO_RS02035"
                     /old_locus_tag="pgaptmp_000407"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGU4427307.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            21239..22816
                     /gene="guaA"
                     /locus_tag="ACHKYO_RS02040"
                     /old_locus_tag="pgaptmp_000408"
     CDS             21239..22816
                     /gene="guaA"
                     /locus_tag="ACHKYO_RS02040"
                     /old_locus_tag="pgaptmp_000408"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGU4427308.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(22911..23450)
                     /gene="yfgI"
                     /locus_tag="ACHKYO_RS02045"
                     /old_locus_tag="pgaptmp_000409"
     CDS             complement(22911..23450)
                     /gene="yfgI"
                     /locus_tag="ACHKYO_RS02045"
                     /old_locus_tag="pgaptmp_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HGU4427309.1"
                     /translation="MKKVFLCAILASLCYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(23466..23984)
                     /gene="yfgH"
                     /locus_tag="ACHKYO_RS02050"
                     /old_locus_tag="pgaptmp_000410"
     CDS             complement(23466..23984)
                     /gene="yfgH"
                     /locus_tag="ACHKYO_RS02050"
                     /old_locus_tag="pgaptmp_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU4427310.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITRTYNET
                     RIQPNTKCPEKS"
     gene            complement(24295..24486)
                     /gene="yfgG"
                     /locus_tag="ACHKYO_RS02055"
                     /old_locus_tag="pgaptmp_000411"
     CDS             complement(24295..24486)
                     /gene="yfgG"
                     /locus_tag="ACHKYO_RS02055"
                     /old_locus_tag="pgaptmp_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HGU4427311.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            24504..24656
                     /locus_tag="ACHKYO_RS02060"
                     /old_locus_tag="pgaptmp_000412"
     CDS             24504..24656
                     /locus_tag="ACHKYO_RS02060"
                     /old_locus_tag="pgaptmp_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4427312.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            24838..27081
                     /gene="pdeF"
                     /locus_tag="ACHKYO_RS02065"
                     /old_locus_tag="pgaptmp_000413"
     CDS             24838..27081
                     /gene="pdeF"
                     /locus_tag="ACHKYO_RS02065"
                     /old_locus_tag="pgaptmp_000413"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HGU4427313.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(27120..28661)
                     /gene="ppx"
                     /locus_tag="ACHKYO_RS02070"
                     /old_locus_tag="pgaptmp_000414"
     CDS             complement(27120..28661)
                     /gene="ppx"
                     /locus_tag="ACHKYO_RS02070"
                     /old_locus_tag="pgaptmp_000414"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HGU4427314.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(28666..30732)
                     /gene="ppk1"
                     /locus_tag="ACHKYO_RS02075"
                     /old_locus_tag="pgaptmp_000415"
     CDS             complement(28666..30732)
                     /gene="ppk1"
                     /locus_tag="ACHKYO_RS02075"
                     /old_locus_tag="pgaptmp_000415"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HGU4427315.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(30902..31540)
                     /gene="purN"
                     /locus_tag="ACHKYO_RS02080"
                     /old_locus_tag="pgaptmp_000416"
     CDS             complement(30902..31540)
                     /gene="purN"
                     /locus_tag="ACHKYO_RS02080"
                     /old_locus_tag="pgaptmp_000416"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGU4427316.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(31540..32577)
                     /gene="purM"
                     /locus_tag="ACHKYO_RS02085"
                     /old_locus_tag="pgaptmp_000417"
     CDS             complement(31540..32577)
                     /gene="purM"
                     /locus_tag="ACHKYO_RS02085"
                     /old_locus_tag="pgaptmp_000417"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGU4427317.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNSNGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            32902..33528
                     /gene="upp"
                     /locus_tag="ACHKYO_RS02090"
                     /old_locus_tag="pgaptmp_000418"
     CDS             32902..33528
                     /gene="upp"
                     /locus_tag="ACHKYO_RS02090"
                     /old_locus_tag="pgaptmp_000418"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGU4427318.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            33614..34903
                     /gene="uraA"
                     /locus_tag="ACHKYO_RS02095"
                     /old_locus_tag="pgaptmp_000419"
     CDS             33614..34903
                     /gene="uraA"
                     /locus_tag="ACHKYO_RS02095"
                     /old_locus_tag="pgaptmp_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HGU4427319.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            34998..35699
                     /gene="hda"
                     /locus_tag="ACHKYO_RS02100"
                     /old_locus_tag="pgaptmp_000420"
     CDS             34998..35699
                     /gene="hda"
                     /locus_tag="ACHKYO_RS02100"
                     /old_locus_tag="pgaptmp_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HGU4427320.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(35837..36196)
                     /gene="arsC"
                     /locus_tag="ACHKYO_RS02105"
                     /old_locus_tag="pgaptmp_000421"
     CDS             complement(35837..36196)
                     /gene="arsC"
                     /locus_tag="ACHKYO_RS02105"
                     /old_locus_tag="pgaptmp_000421"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HGU4427321.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(36217..37680)
                     /gene="bepA"
                     /locus_tag="ACHKYO_RS02110"
                     /old_locus_tag="pgaptmp_000422"
     CDS             complement(36217..37680)
                     /gene="bepA"
                     /locus_tag="ACHKYO_RS02110"
                     /old_locus_tag="pgaptmp_000422"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HGU4427322.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            37893..38954
                     /gene="yfgO"
                     /locus_tag="ACHKYO_RS02115"
                     /old_locus_tag="pgaptmp_000423"
     CDS             37893..38954
                     /gene="yfgO"
                     /locus_tag="ACHKYO_RS02115"
                     /old_locus_tag="pgaptmp_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU4427323.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(38992..39840)
                     /gene="focB"
                     /locus_tag="ACHKYO_RS02120"
                     /old_locus_tag="pgaptmp_000424"
     CDS             complement(38992..39840)
                     /gene="focB"
                     /locus_tag="ACHKYO_RS02120"
                     /old_locus_tag="pgaptmp_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HGU4427324.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQEP"
     gene            complement(39862..41880)
                     /gene="hyfR"
                     /locus_tag="ACHKYO_RS02125"
                     /old_locus_tag="pgaptmp_000425"
     CDS             complement(39862..41880)
                     /gene="hyfR"
                     /locus_tag="ACHKYO_RS02125"
                     /old_locus_tag="pgaptmp_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HGU4427325.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVTLRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADADAWRSMTDLQESLQQENH
                     QLSEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQ
                     LSPRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEI
                     GDLPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFY
                     RLNVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNV
                     RELENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEE
                     ERQRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(41910..42323)
                     /gene="hyfJ"
                     /locus_tag="ACHKYO_RS02130"
                     /old_locus_tag="pgaptmp_000426"
     CDS             complement(41910..42323)
                     /gene="hyfJ"
                     /locus_tag="ACHKYO_RS02130"
                     /old_locus_tag="pgaptmp_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HGU4427326.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(42316..43074)
                     /gene="hyfI"
                     /locus_tag="ACHKYO_RS02135"
                     /old_locus_tag="pgaptmp_000427"
     CDS             complement(42316..43074)
                     /gene="hyfI"
                     /locus_tag="ACHKYO_RS02135"
                     /old_locus_tag="pgaptmp_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HGU4427327.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLHDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(43071..43610)
                     /gene="hyfH"
                     /locus_tag="ACHKYO_RS02140"
                     /old_locus_tag="pgaptmp_000428"
     CDS             complement(43071..43610)
                     /gene="hyfH"
                     /locus_tag="ACHKYO_RS02140"
                     /old_locus_tag="pgaptmp_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HGU4427328.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            complement(43620..45335)
                     /gene="hyfG"
                     /locus_tag="ACHKYO_RS02145"
                     /old_locus_tag="pgaptmp_000429"
     CDS             complement(43620..45335)
                     /gene="hyfG"
                     /locus_tag="ACHKYO_RS02145"
                     /old_locus_tag="pgaptmp_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HGU4427329.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLAMPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(45325..46905)
                     /gene="hyfF"
                     /locus_tag="ACHKYO_RS02150"
                     /old_locus_tag="pgaptmp_000430"
     CDS             complement(45325..46905)
                     /gene="hyfF"
                     /locus_tag="ACHKYO_RS02150"
                     /old_locus_tag="pgaptmp_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HGU4427330.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(46910..47560)
                     /gene="hyfE"
                     /locus_tag="ACHKYO_RS02155"
                     /old_locus_tag="pgaptmp_000431"
     CDS             complement(46910..47560)
                     /gene="hyfE"
                     /locus_tag="ACHKYO_RS02155"
                     /old_locus_tag="pgaptmp_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HGU4427331.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(47572..49009)
                     /gene="hyfD"
                     /locus_tag="ACHKYO_RS02160"
                     /old_locus_tag="pgaptmp_000432"
                     /pseudo
     CDS             complement(47572..49009)
                     /gene="hyfD"
                     /locus_tag="ACHKYO_RS02160"
                     /old_locus_tag="pgaptmp_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(49026..49973)
                     /gene="hyfC"
                     /locus_tag="ACHKYO_RS02165"
                     /old_locus_tag="pgaptmp_000433"
     CDS             complement(49026..49973)
                     /gene="hyfC"
                     /locus_tag="ACHKYO_RS02165"
                     /old_locus_tag="pgaptmp_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HGU4427332.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(49984..52001)
                     /gene="hyfB"
                     /locus_tag="ACHKYO_RS02170"
                     /old_locus_tag="pgaptmp_000434"
                     /pseudo
     CDS             complement(49984..52001)
                     /gene="hyfB"
                     /locus_tag="ACHKYO_RS02170"
                     /old_locus_tag="pgaptmp_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(52001..52618)
                     /gene="hyfA"
                     /locus_tag="ACHKYO_RS02175"
                     /old_locus_tag="pgaptmp_000435"
     CDS             complement(52001..52618)
                     /gene="hyfA"
                     /locus_tag="ACHKYO_RS02175"
                     /old_locus_tag="pgaptmp_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HGU4427333.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRREPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(52871..53341)
                     /gene="bcp"
                     /locus_tag="ACHKYO_RS02180"
                     /old_locus_tag="pgaptmp_000436"
     CDS             complement(52871..53341)
                     /gene="bcp"
                     /locus_tag="ACHKYO_RS02180"
                     /old_locus_tag="pgaptmp_000436"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HGU4427334.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(53341..53913)
                     /gene="gcvR"
                     /locus_tag="ACHKYO_RS02185"
                     /old_locus_tag="pgaptmp_000437"
     CDS             complement(53341..53913)
                     /gene="gcvR"
                     /locus_tag="ACHKYO_RS02185"
                     /old_locus_tag="pgaptmp_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HGU4427335.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            54059..54937
                     /gene="dapA"
                     /locus_tag="ACHKYO_RS02190"
                     /old_locus_tag="pgaptmp_000438"
     CDS             54059..54937
                     /gene="dapA"
                     /locus_tag="ACHKYO_RS02190"
                     /old_locus_tag="pgaptmp_000438"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGU4427336.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            54954..55988
                     /gene="bamC"
                     /locus_tag="ACHKYO_RS02195"
                     /old_locus_tag="pgaptmp_000439"
     CDS             54954..55988
                     /gene="bamC"
                     /locus_tag="ACHKYO_RS02195"
                     /old_locus_tag="pgaptmp_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HGU4427337.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VSVFQAAFSK"
     gene            56201..56914
                     /gene="purC"
                     /locus_tag="ACHKYO_RS02200"
                     /old_locus_tag="pgaptmp_000440"
     CDS             56201..56914
                     /gene="purC"
                     /locus_tag="ACHKYO_RS02200"
                     /old_locus_tag="pgaptmp_000440"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGU4427338.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            57068..57295
                     /gene="vapB"
                     /locus_tag="ACHKYO_RS02205"
                     /old_locus_tag="pgaptmp_000441"
     CDS             57068..57295
                     /gene="vapB"
                     /locus_tag="ACHKYO_RS02205"
                     /old_locus_tag="pgaptmp_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HGU4427339.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            57295..57693
                     /locus_tag="ACHKYO_RS02210"
                     /old_locus_tag="pgaptmp_000442"
     CDS             57295..57693
                     /locus_tag="ACHKYO_RS02210"
                     /old_locus_tag="pgaptmp_000442"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4427340.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            57840..58703
                     /gene="ypfJ"
                     /locus_tag="ACHKYO_RS02215"
                     /old_locus_tag="pgaptmp_000443"
     CDS             57840..58703
                     /gene="ypfJ"
                     /locus_tag="ACHKYO_RS02215"
                     /old_locus_tag="pgaptmp_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HGU4427341.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            58718..60733
                     /gene="tmcA"
                     /locus_tag="ACHKYO_RS02220"
                     /old_locus_tag="pgaptmp_000444"
     CDS             58718..60733
                     /gene="tmcA"
                     /locus_tag="ACHKYO_RS02220"
                     /old_locus_tag="pgaptmp_000444"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HGU4427342.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERLRDRITQWQFFH"
     gene            60807..61505
                     /gene="ypfH"
                     /locus_tag="ACHKYO_RS02225"
                     /old_locus_tag="pgaptmp_000445"
     CDS             60807..61505
                     /gene="ypfH"
                     /locus_tag="ACHKYO_RS02225"
                     /old_locus_tag="pgaptmp_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4427343.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(61615..61815)
                     /gene="ypfN"
                     /locus_tag="ACHKYO_RS02230"
                     /old_locus_tag="pgaptmp_000446"
     CDS             complement(61615..61815)
                     /gene="ypfN"
                     /locus_tag="ACHKYO_RS02230"
                     /old_locus_tag="pgaptmp_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HGU4427344.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(61843..62970)
                     /gene="dapE"
                     /locus_tag="ACHKYO_RS02235"
                     /old_locus_tag="pgaptmp_000447"
     CDS             complement(61843..62970)
                     /gene="dapE"
                     /locus_tag="ACHKYO_RS02235"
                     /old_locus_tag="pgaptmp_000447"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HGU4427345.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(62974..63330)
                     /gene="yffB"
                     /locus_tag="ACHKYO_RS02240"
                     /old_locus_tag="pgaptmp_000448"
     CDS             complement(62974..63330)
                     /gene="yffB"
                     /locus_tag="ACHKYO_RS02240"
                     /old_locus_tag="pgaptmp_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HGU4427346.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            63711..63770
                     /gene="ypfM"
                     /locus_tag="ACHKYO_RS02245"
                     /old_locus_tag="pgaptmp_000449"
     CDS             63711..63770
                     /gene="ypfM"
                     /locus_tag="ACHKYO_RS02245"
                     /old_locus_tag="pgaptmp_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HGU4427347.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(63869..66982)
                     /gene="acrD"
                     /locus_tag="ACHKYO_RS02250"
                     /old_locus_tag="pgaptmp_000450"
     CDS             complement(63869..66982)
                     /gene="acrD"
                     /locus_tag="ACHKYO_RS02250"
                     /old_locus_tag="pgaptmp_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HGU4427348.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPKLTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(67146..68846)
                     /gene="narQ"
                     /locus_tag="ACHKYO_RS02255"
                     /old_locus_tag="pgaptmp_000451"
     CDS             complement(67146..68846)
                     /gene="narQ"
                     /locus_tag="ACHKYO_RS02255"
                     /old_locus_tag="pgaptmp_000451"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HGU4427349.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            69052..71031
                     /gene="aegA"
                     /locus_tag="ACHKYO_RS02260"
                     /old_locus_tag="pgaptmp_000452"
     CDS             69052..71031
                     /gene="aegA"
                     /locus_tag="ACHKYO_RS02260"
                     /old_locus_tag="pgaptmp_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HGU4427350.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVGVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            71099..71674
                     /gene="nudK"
                     /locus_tag="ACHKYO_RS02265"
                     /old_locus_tag="pgaptmp_000453"
     CDS             71099..71674
                     /gene="nudK"
                     /locus_tag="ACHKYO_RS02265"
                     /old_locus_tag="pgaptmp_000453"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HGU4427351.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            71800..72843
                     /gene="ypfG"
                     /locus_tag="ACHKYO_RS02270"
                     /old_locus_tag="pgaptmp_000454"
     CDS             71800..72843
                     /gene="ypfG"
                     /locus_tag="ACHKYO_RS02270"
                     /old_locus_tag="pgaptmp_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HGU4427352.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(72939..74942)
                     /gene="tkt"
                     /locus_tag="ACHKYO_RS02275"
                     /old_locus_tag="pgaptmp_000455"
     CDS             complement(72939..74942)
                     /gene="tkt"
                     /locus_tag="ACHKYO_RS02275"
                     /old_locus_tag="pgaptmp_000455"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU4427353.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(74962..75912)
                     /gene="tal"
                     /locus_tag="ACHKYO_RS02280"
                     /old_locus_tag="pgaptmp_000456"
     CDS             complement(74962..75912)
                     /gene="tal"
                     /locus_tag="ACHKYO_RS02280"
                     /old_locus_tag="pgaptmp_000456"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU4427354.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            76201..78480
                     /gene="maeB"
                     /locus_tag="ACHKYO_RS02285"
                     /old_locus_tag="pgaptmp_000457"
     CDS             76201..78480
                     /gene="maeB"
                     /locus_tag="ACHKYO_RS02285"
                     /old_locus_tag="pgaptmp_000457"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HGU4427355.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            78772..79107
                     /gene="eutS"
                     /locus_tag="ACHKYO_RS02290"
                     /old_locus_tag="pgaptmp_000458"
     CDS             78772..79107
                     /gene="eutS"
                     /locus_tag="ACHKYO_RS02290"
                     /old_locus_tag="pgaptmp_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HGU4427356.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            79120..79599
                     /gene="eutP"
                     /locus_tag="ACHKYO_RS02295"
                     /old_locus_tag="pgaptmp_000459"
     CDS             79120..79599
                     /gene="eutP"
                     /locus_tag="ACHKYO_RS02295"
                     /old_locus_tag="pgaptmp_000459"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HGU4427357.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            79574..80275
                     /gene="eutQ"
                     /locus_tag="ACHKYO_RS02300"
                     /old_locus_tag="pgaptmp_000460"
     CDS             79574..80275
                     /gene="eutQ"
                     /locus_tag="ACHKYO_RS02300"
                     /old_locus_tag="pgaptmp_000460"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HGU4427358.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            80272..81075
                     /gene="eutT"
                     /locus_tag="ACHKYO_RS02305"
                     /old_locus_tag="pgaptmp_000461"
     CDS             80272..81075
                     /gene="eutT"
                     /locus_tag="ACHKYO_RS02305"
                     /old_locus_tag="pgaptmp_000461"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HGU4427359.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            81072..82088
                     /gene="pta"
                     /locus_tag="ACHKYO_RS02310"
                     /old_locus_tag="pgaptmp_000462"
     CDS             81072..82088
                     /gene="pta"
                     /locus_tag="ACHKYO_RS02310"
                     /old_locus_tag="pgaptmp_000462"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU4427360.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            82127..82420
                     /gene="eutM"
                     /locus_tag="ACHKYO_RS02315"
                     /old_locus_tag="pgaptmp_000463"
     CDS             82127..82420
                     /gene="eutM"
                     /locus_tag="ACHKYO_RS02315"
                     /old_locus_tag="pgaptmp_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HGU4427361.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            82527..82814
                     /gene="eutN"
                     /locus_tag="ACHKYO_RS02320"
                     /old_locus_tag="pgaptmp_000464"
     CDS             82527..82814
                     /gene="eutN"
                     /locus_tag="ACHKYO_RS02320"
                     /old_locus_tag="pgaptmp_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HGU4427362.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            82826..>84081
                     /locus_tag="ACHKYO_RS02325"
                     /old_locus_tag="pgaptmp_000465"
     CDS             82826..>84081
                     /locus_tag="ACHKYO_RS02325"
                     /old_locus_tag="pgaptmp_000465"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGU4427363.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVK"
BASE COUNT        20266 a        21449 c        22593 g        19773 t
ORIGIN      
        1 ccaccagtaa attgtcgccg gagagactcc ggttattatt tacttgggaa aacagcgcac
       61 cagaaacaat aagtacgata cagaagcacc gtatcttcat tgtgcggtcc atgcgaagac
      121 cgcatcacga cagcaccact ggcgatattg ccgcgatagc aagcgctgag acgcgcttgc
      181 tctaacggtc gcggaacatc ctttccttaa gccagcgccc gcttgcgaga cggcggaaag
      241 ggccgcagtg gataatacac taacagaaca ataataactc tcttttgctt gacaaaaaga
      301 gagttactgg cgagtattgt ctccgctgag acgcgcttgc attactacgg taatacaacc
      361 tgctggaaag gccgcgaacc agaccagcaa taaaaaaccg ccaaattcgg cggtttttta
      421 ttgcctgtaa aatacttatt tcactggctc aaccggtaaa tctgcccgcg cgccccattc
      481 actccatgcg ccgtcgtaca gtttcacgtt tggcacatcc agcgtcgcga gtgctaacaa
      541 aaccacggct gccgttacac cagagccgca gctgacgata attggtttgt cgtagctgac
      601 gccgcgacca aaaaatatcg catccagttc atcggtcgtt tttagttcgc cttcgcgcac
      661 cagttccgtc cacggaacat tcagtgctcc gggaatatgt ccgcgacgta aacctgggcg
      721 aggttcatca acttctgcgt taaaacgtgc agccgggcgg gcatcaataa tttgcgccgt
      781 attttcatgg cttgccaata atacatcggt tactttcacc acggcttcag gattaaacgc
      841 ggcgttaaac tctccttccg gcagctctac tgcaccttct tctaacagca gatcatcgcg
      901 ctgccagcct gcaagtccac cccccagaat cgacactttc tctacaccaa aggtgcgcag
      961 catccaccat gctcgtgggg ctgagaaaag attaccttcg tcatagacaa tcaggtgctt
     1021 atcctggtta acgcctaatt cacgcatcgc cacggcgaac gtttccgggc gcggcagcat
     1081 gtgcggaagc ggggaagtgt gatcagaaag cgcttcgata tcaaaaaaca ctgcgccggg
     1141 aatatgtcca ttcagatact cctgagcaac gttacgatct tcctgtccag gcgacgccat
     1201 gcgggcatca ataatctgaa tttccgggtc atcaatatgt tcggcgagcc agtcggctcc
     1261 tacaaaccat gtcgtggaca taggcatctc catttttgcc ggggttaggc taaattgtcg
     1321 gatgtttagt gaaaagcgac aagcgaaaga ggctgatttg gctgtctgag ccacaattct
     1381 cactaaaatt cggattgtat gggcgtgata ctgaattaca taacgtaaat tctcacgcat
     1441 aataattatt cgacgttgct aacgggccat tgtggcaggg atgaaaaatg aaaaagttac
     1501 gcgtagccgc ctgcatgcta atgctggcgc tggcagggtg cgacaacaac gataacgcgc
     1561 caacagcggt gaaaaaagat gcgccttctg aagttactaa agcggcctct tcagaaaacg
     1621 cgagttcagc aaaactctcc gtgccggaga gacaaaaact ggcccaacag agtgccggta
     1681 aggtgctgac attgctggat ctctctgaag tccaacttga tggtgcagcc acgctggtgc
     1741 tgacgttctc catccctctc gacccggatc aggatttctc acgcgttatt catgtcgtcg
     1801 ataaaaaaag cggcaaagtg gatggtgcct gggagctgtc agataatctt aaagagttac
     1861 gtttacgcca cctcgaaccg aaacgtgatt tgatcgttac tattggcaag gaggtcaaag
     1921 cactcaacaa cgcaaccttc agtaaagatt acgaaaaaac tataactacc cgcgacatcc
     1981 aacccagcgt cggttttgcc agccgtggtt cgctgctgcc tggcaaagtc gttgaagggc
     2041 tgccggtaat ggcgctcaac gttaataatg tcgatgttaa cttcttccgc gttaagccag
     2101 aatctctgcc agcattcatt agccaatggg aataccgcaa ttcgctggcg aactggcagt
     2161 cagacaaact gctgcagatg gcggatctgg tctacaccgg acggtttgat ctcaatcctg
     2221 cgcgtaacac ccgtgaaaaa ttattgctgc cgctgggcga tatcaaaccg cttcagcagg
     2281 cgggcgtgta tctggctgtg atgaatcagg ctggacgtta cgattacagt aatcccgcga
     2341 cgctgtttac gttaagtgat atcggcgttt cagctcaccg ttatcacaat cgtctggata
     2401 tctttaccca aagtctggaa aacggcgcgg cccagcaagg aattgaagtc tctttattaa
     2461 atgagaaagg gcagactctg actcaggcaa ccagtgacgc tcaggggcat gtgcagctgg
     2521 aaaatgataa aaacgcggca ttactgttgg cgcgtaaaga cggtcagaca acgctactcg
     2581 atttaaaact tccggcgctg gacttagcag aatttaacat tgctggcgcg ccaggctata
     2641 gcaaacagtt tttcatgttt ggcccacgcg atctttatcg cccaggtgaa acggtaatcc
     2701 tcaatggttt gctgcgtgat gcagacggta aagcgttgcc caatcaaccc attaagttag
     2761 acgtgattaa acccgatggg caggtactca ggagcgtcgt tagtcagccg gagaatggcc
     2821 tctaccactt tacctggcca ctcgatagca atgcggcaac cggtatgtgg catattcgcg
     2881 ctaacacggg cgataaccag tatcggatgt gggatttcca cgtcgaagat tttatgccag
     2941 agcgcatggc gctgaatctg accggtgaga aaaccccgct aacgccgaaa gatgaagtga
     3001 aattctccgt ggtggggtac tacctgtatg gtgcacctgc taatggtaat actttgcaag
     3061 ggcaactttt cctgcgccca ctgcgtgaag ctgtgtcagc cttacctggt tttgaattcg
     3121 gcgatatagc tgccgaaaat ctttcccgca cgctggatga agttcagttg acgctggatg
     3181 ataaagggcg cggcgaagtt tctacagaaa gccagtggaa ggaaacgcat tccccattac
     3241 aggttatttt ccagggtagt ttgctggaat cgggcggtcg cccggtgacg cgccgcgctg
     3301 agcaggctat ctggcctgcc gatgcattgc cggggatccg tccgcagttc gcctcgaaat
     3361 cggtttacga ttatcgtact gacagcacgg tgaaacagcc cattgttgat gaaggcagta
     3421 acgctgcttt tgacatcgtt tatagcgatg cgcaaggcgt gaaaaaagcc gtgtcgggct
     3481 tgcaggtgcg cctgattcgc gaacgccgcg attactactg gaactggtca gaagatgaag
     3541 gctggcagtc acagtttgat caaaaagatc tgatcgaaaa tgaacaaact ctggatctga
     3601 aagcggacga aaccggcaag gtcagttttc cggtagagtg gggcgcttat cgtctggaag
     3661 tcaaagcgcc gaatgaagcg gtcagtagtg ttcgtttctg ggctggctat agctggcagg
     3721 acaacagcga cggtagcggc gcagtgcgac ccgaccgtgt cacgctgaaa ctggataaag
     3781 ccagttatcg ccctggcgac accattaagt tgcatatcgc cgcgccaacg gcgggtaaag
     3841 gttatgcgat ggtcgagtcc agtgaagggc cgctgtggtg gcaagagatt gatgttcggg
     3901 ctcaagggct ggatctgacg attccggtcg ataaaacctg gaatcgtcat gatctgtatt
     3961 taagtacgct ggtggtacgt cctggcgata aatctcgctc cgcgacgcca aaacgcgcgg
     4021 ttggtgtgtt gcatctgccg cttggcgatg aaaaccgtcg cctcgatctg gcgctggaaa
     4081 caccagcaaa aatgcgtccc aatcaaccat taaccgtgaa aattaaagcc agcactaaaa
     4141 atggcgagaa gcctaaacag gtgaatgtgc tggtgtctgc cgttgatagt ggtgtgctga
     4201 atattactga ctacgtcacg ccagatccgt ggcaggcgtt ctttggtcag aaacgctatg
     4261 gcgcagacat ttacgatatt tacggtcagg ttattgaagg tcaggggcgt ctggcagctc
     4321 tgcgtttcgg tggcgatggt gatgagctga aacgtggtgg taaaccgccg gtcaatcacg
     4381 tcaatattgt cgcgcagcag gcgctgccgg taacgctcaa cgaacagggc gaaggctcgg
     4441 ttacactgcc gattggcgat tttaacggtg aattgcgcgt catggcgcaa gcctggacgg
     4501 cagatgattt cggtagcaac gaaagcaaag tgatagttgc cgcaccggtg attgctgaac
     4561 tgaacatgcc gcgctttatg gcgagtggcg atacctcgcg tctgacgctg gatatcacta
     4621 atcttaccga taaaccgcaa aaactgaacg ttgccctgac cgccagtggt ttgcttgaac
     4681 tggtcagcga ttcacccgca gccgttgaat tagcgccagg tgtgcgtact acgctgttta
     4741 tcccggtgcg agcattgccg ggttatggcg atggagaaat tcaggccacc attagcgggt
     4801 tagcgttacc gggtgaaacc gttgccgatc agcataagca gtggaaaatc ggcgtccgtc
     4861 cggcgttccc ggcacaaacg gttaattacg gtacggcgtt acagcctggt gagacatggg
     4921 cgattccggc ggatggattg caaaacttct cgcctgttac gctggaaggg caattgttgt
     4981 tgagcggcaa accaccgctg aacattgcac gttatatcaa agagttaaaa gcgtatccgt
     5041 acggctgtct tgagcaaacc gccagcggcc tgttcccgtc actttatacc aacgcagccc
     5101 aactgcaggc gttgggcatc aaaggcgaca gtgatgagaa acgccgtgca tcggtcgata
     5161 tcggcatttc ccgtttgctg caaatgcaac gtgataacgg cggctttgcg ctgtgggata
     5221 aaaacggtga cgaagagtac tggctgacgg cttacgtgat ggatttcctg gtccgcgcag
     5281 gcgaacaggg ttacagcgtg ccgacagacg ccattaaccg gggtaatgag cgtctgctgc
     5341 gctatttaca agatccgggc atgatgtcga tcccgtacgc ggataatctc aaagccagta
     5401 aattcgccgt tcagtcttac gctgcgctgg tgctggcccg tcagcaaaaa gcaccactgg
     5461 gtgcgctgcg tgaaatctgg gagcatcgtg cagatgccgc ttctggttta ccgctgctgc
     5521 aacttggcgt tgcgctgaaa accatgggtg atgcaacgcg tggtgaagaa gcgattgtgc
     5581 tggcgctgaa aacgccgaga aatagtgatg agcggatatg gctgggtgat tacggtagtc
     5641 cactgcgcga caacgcgtta atgctctcct tgctggaaga aaataaacta ctacccgatg
     5701 agcagtacac tttgctgaac acactttcgc agcaggcgtt tggtgaacgc tggctatcga
     5761 cgcaggaaag taacgcgttg ttcctggctg cccgtacgat tcaggattta ccgggtaaat
     5821 ggcaggcgca aacctctttc tcagctgagc cgctgacagg cgagaaaacg ctaaacagca
     5881 atctgaatag cgatcaactt gccaccttgc aggtgagaaa cagtggcgat cagccgttat
     5941 ggttgcgtat ggatgccagc ggttatccgc aatccgcacc tttaccggcg aacaatgtgc
     6001 tgcaaatcga gcgtcatatt cttggtactg atggtaagag caaatcgctg gactcgttac
     6061 gtagcggcga tctggtgctg gtctggttgc aggtaaaagc cagtaacagc gtgccggatg
     6121 cgttagtcgt ggatctgctg cctgcgggtc tggaactgga aaatcagaat ctggcgaacg
     6181 gtagcgccag cctggagcaa agtggtggcg aagtgcaaaa cttactgaac cagatgcagc
     6241 aggcgagtat taagcacatt gagttccgtg acgatcgctt tgtggcggcg gttgccgttg
     6301 atgaatacca accggtaacg ctggtgtatc tggcgcgggc ggtgacgccg ggaacgtatc
     6361 aggtaccgca accgatggtg gaatcaatgt atgttcccca atggcgggcg accggcacgg
     6421 ctgaagatct gctgattgtc cgaccgtaaa tgcctcgctt gttaaccaaa cgcggctact
     6481 ggataacgct ggcagctgcg ccatttctcc tttttcttgc agcgtgggga gcagataaac
     6541 tctggcctct accgctgcat gaagtcaatc ccgcacgagt ggtcgtggcg caggatggta
     6601 cgccgctatg gcgcttcgcc gatgctgacg gcatctggcg ttatccggta acaatcgaag
     6661 atgtttctcc acgttacctt gaagcgctga tcaattatga agatcgctgg ttctggaagc
     6721 atccgggggt gaatccattc tcggtggcgc gcgcagcatg gcaagatctc acttcgggac
     6781 gggttatttc cggtggcagc acgctcacta tgcaggttgc tcgtctgctt gatcctcacc
     6841 ccaaaacatt tggcggcaaa attcgccagc tctggcgcgc gttgcaactg gaatggcatc
     6901 tgtctaagcg tgaaattctg accttgtatc ttaaccgcgc tccgtttggc ggtacgttgc
     6961 aggggatcgg tgcggcaagt tgggcttatc tcggaaagtc gcctgcgaat ttaagctatt
     7021 ccgaggcggc aatgctggcg gttttgccgc aagcgcccag ccgtcttcgc ccggatcgtt
     7081 ggccggagcg tgccgaagcc gcgcgtaata aagtgctcga acggatggcc gtgcaaggtg
     7141 tgtggtcccg tgagcaggta aaagagtcaa gggaagaacc catctggctg gcaccccgac
     7201 aaatgccgca actggcaccg ctgttttcgc gcatgatgct cggtaaaagc aaaagcgaca
     7261 aaatcgttac tacgctggat gccggtcttc aacgacgtct ggaagaactg gcgcaaaact
     7321 ggaaagggcg gttgccaccg cgcagctcac tggcgatgat cgtggttgat cataccgata
     7381 tgcgtgttcg cggctgggtg ggatcggttg atctcaacga tgattcacgc tttggtcatg
     7441 ttgatatggt caatgcgatc cgatcgccag gatcggtgct caaaccgttt gtttatggtc
     7501 tggcgctgga tgaaggcttg atccacccgg catcactgct gcaagacgtc ccccggcgca
     7561 ccggtgatta tcgaccaggt aactttgata gcggttttca tggcccgatc agcatgagcg
     7621 aggcgctggt gcgctcgctg aacttacctg ctgtgcaggt gctggaagcc tatggaccga
     7681 aacggtttgc ggcaaagtta cgcaatgttg gattgccgtt atatttgccc aacggtgctg
     7741 cgccgaatct ttcactcatt ctcggcggcg ctggtgcaaa actggaagat atggcggcag
     7801 cgtataccgc gtttgctcgc cacggcaagg caggcaaatt gcgcttacag cctgacgatc
     7861 cgctgcttga acggccttta atgtcgtcgg gggcggcgtg gatcattcgc cggattatgg
     7921 ctgatgaagc gcaacccttg ccggatagtg ccttgccgcg cgtcgcccca ctggcatgga
     7981 aaacgggcac cagctatggc tatcgtgacg cctgggcgat tggggttaac gctcgctatg
     8041 tcattgggat ctggactggc agaccggacg gcacgcccgt tgttggtcag tttggctttg
     8101 ccagtgccgt accattgttg aatcaggtca ataatatctt actgtcgcgc agtgcgaatc
     8161 tgccggaaga cccgcgcccg aactcagtca ctcgtggcgt tatctgctgg ccgggcggac
     8221 agtctttgcc ggaaggtgac ggtaactgtc gccgccgcct ggcaacctgg ctgctggacg
     8281 ggagtcagcc gccaactcta ttactgccgg agcaggaagg cattaatggc attcgtttcc
     8341 ccatctggct ggatgaaaat ggcaaacgtg ttgccgctga ttgcccgcaa gcgcgacaag
     8401 aaatgataaa tgtctggccg ctaccgctgg aaccctggct gcccgcatca gaacgtcgcg
     8461 ctgtgcgctt accaccggcc tcgacaattt gcccgccata cggtcacgat gccaagctcc
     8521 ctctgcagct gacaggcgtt cgcgatggcg cgattattaa acgtttaccg ggcgcagcgg
     8581 aagcgacttt gccgttgcaa tcaagtggag gggcaggtga acgctggtgg tttctgaatg
     8641 gcgaaccgtt aactgaacgc gggcgcaacg tgaccctgca tttgacggat aaaggtgatt
     8701 atcaattgct ggtgatggat gatgtggggc aaatcgcgac agtgaaattt gtcatgcaat
     8761 agtctgtttt gcccctgatt gttgctaaaa ctcattttat tttaaaaaaa tgttaccttc
     8821 atcaatagtc aacggccctg ttgctcatta taatccgcgc catctcgtac gctggtacag
     8881 acaacaacag aacaatttac agaggtaaaa atggctattg aacgtacttt ttccatcatc
     8941 aaaccgaacg cggtagcaaa aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca
     9001 gggttcaaaa ttgtcggcac caaaatgctg cacctgaccg ttgaacaggc acgtggcttt
     9061 tatgctgaac acgatggaaa accgttcttt gatggtctgg ttgaattcat gacctctggc
     9121 ccgatcgtgg tttccgtgct ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg
     9181 ggcgcgacca atccggcaaa cgcactggct ggtactctgc gcgctgatta cgctgacagc
     9241 ctgaccgaaa acggtaccca cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct
     9301 tatttctttg gcgaaggcga agtgtgcccg cgcacccgtt aataatttcg taaatgccgc
     9361 gtgcaaacgt ggcatccgtg cgccagaatt tgtacaatgc agcgcccccg gacgagcagc
     9421 cgctcaccgg ggcgtttctt ttttcaatcc tccaggggcc ataacgtgta ataacgaggc
     9481 cggaatagca tatgtctgaa caattagtca cacctgaaaa cgtcaccacg aaagatggaa
     9541 aaatcaacct gctggatctc aaccgtcagc agatgcggga gttttttaaa gatttaggtg
     9601 aaaaaccctt ccgcgccgat caggtgatga agtggatgta tcactattgc tgcgacaact
     9661 ttgatgagat gaccgacatc aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa
     9721 tccgcgcacc ggaagtggtt gaagaacagc gttcatccga cggcaccatt aaatgggcga
     9781 tcgccgttgg cgatcagcgc gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc
     9841 tctgcgtctc ttcgcaggtg gggtgtgcgc tggagtgtaa attctgttcc accgcccagc
     9901 agggctttaa ccgcaacctg cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga
     9961 aaatcgtcgg cgcagcaaaa gtcaccggtc agcgcccaat cactaacgta gtgatgatgg
    10021 gcatgggcga gccgctgctc aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg
    10081 atgatttcgg ttttggcctg tctaaacgtc gcgtcacgct ttctacttcc ggtgtagttc
    10141 cggcgctgga taaactgggc gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc
    10201 cgaacgacga aattcgtgac gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt
    10261 tccttgctgc ggtgcgccgt tatctggaga aatccaacgc caatcagggc cgtgtcacta
    10321 ttgagtatgt gatgcttgac cacgttaacg acggcactga acacgcgcac caactggcgg
    10381 aactgctgaa agatacgccg tgtaagatca acctgatccc gtggaacccc ttcccgggcg
    10441 cgccgtatgg acgcagctcg aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct
    10501 acggtttcac cactattgtg cgtaaaaccc gtggtgatga tatcgatgct gcctgtggtc
    10561 agttggcggg cgatgttatc gaccgtacga aacgtaccct gcgtaaacgg atgcagggtg
    10621 aagccatcga cattaaagcg gtctgatagc cattacgcca cggttacttt atgtaccgtg
    10681 gcgtaatctt ttatgaatga agtattaagt tgcaattgtc tggtcaatat taacggtgcg
    10741 tttttgctga ctttaaggca gtatgtaacg atgcaacagt aagttagcct tagtgaatgt
    10801 gggctttgtc acgagcacac agacggtctt atactgtatg ataacggtta acttaacgga
    10861 tgtttcgcgg tgtgggtgag catgatggtt caccggcatc tcaattctca tttaaacgta
    10921 cctgcagcga atgaatactg aagccacgca cgaccaaaat gaagcactta ctaccggcgc
    10981 tcgcctgcgt aatgctcgcg aacaactagg acttagtcag caggccgttg ccgagcgact
    11041 ttgcctgaag gtttccacgg tacgcgacat tgaagaagat aaggcacccg ccgatcttgc
    11101 ttcaacattc ctgcgcggat atatccgctc ttatgcgcgt ctggtacata ttccagaaga
    11161 agaactgctg ccagggctgg aaaagcaggc tccactacgg gctgcaaaag ttgcgcctat
    11221 gcagagtttt tccctcggta aacgccgcaa aaaacgtgac ggctggctga tgaccttcac
    11281 ctggctggtg ttgtttgtgg ttatcggcct gagcggtgcc tggtggtggc aagaccacaa
    11341 agctcagcag gaagagatca ccactatggc cgatcaatct tcggcggaac tgagcagtaa
    11401 tagcgagcag gggcagagtg ttccgttaaa tacgtcgaca actacagacc cggctacaac
    11461 gtccacgccg ccagcgtctg tggatactac cgcaaccaac acgcaaacac ctgccgtaac
    11521 tgcgccagca ccagctgttg atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa
    11581 tgttgatacc gccgcgaccc cggcaccaac ggcaacaaca acgccagatg gcgctgcgcc
    11641 gttgccaacc gatcaggcag gcgtgactac gccggcggct gatccgaatg cgctggtgat
    11701 gaactttact gccgattgct ggctggaggt tactgatgct accggtaaaa aattgtttag
    11761 cggtatgcag cgtaaagacg gtaatttgaa cttaaccggc caggcaccat acaaactgaa
    11821 aattggtgcg ccagccgcag tacagatcca gtatcaaggg aaacctgtcg atctgagtcg
    11881 ttttatcaga actaaccagg ttgcgcgtct gaccctcaat gccgaacaat caccggcgca
    11941 gtaacagacg ggtaacgcgg gagatttttc atgcataacc aggctccaat tcaacgtaga
    12001 aaatcaacac gtatttacgt tgggaatgtg ccgattggcg atggtgctcc catcgccgta
    12061 cagtccatga ccaatacgcg tacgacagac gtcgaagcaa cggtcaatca aatcaaggcg
    12121 ctggaacgcg ttggcgctga tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa
    12181 gcgttcaaac tcatcaaaca gcaggttaac gtgccgctgg tggctgacat ccacttcgac
    12241 tatcgcattg cactgaaagt agcggaatac ggcgtcgatt gtctgcgtat taaccctggc
    12301 aatatcggta atgaagagcg tattcgcatg gtggttgact gtgcgcgcga taaaaacatt
    12361 ccgatccgta ttggcgttaa cgccggatcg ctggaaaaag atctgcaaga aaagtatggc
    12421 gaaccgacgc cgcaggcgtt gctggaatcc gccatgcgcc atgttgatca tctcgatcgc
    12481 ctgaacttcg atcagttcaa agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag
    12541 tcttatcgtt tgctggcaaa acagatcgat cagccgctgc atctggggat caccgaagcc
    12601 ggtggtgcac gcagcggggc agtaaaatcc gccattggtt taggtctgct gctgtctgaa
    12661 ggcatcggcg acacgctgcg cgtatcactg gcggccgatc cggtcgaaga gatcaaagtc
    12721 ggtttcgata ttttgaaatc gctgcgtatc cgttcgcgcg ggatcaactt catcgcctgc
    12781 ccgacctgtt cgcgtcagga atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc
    12841 ctggaagata tcatcactcc gatggacgtt tcgattatcg gctgcgtggt gaatggccca
    12901 ggtgaggcgc tggtttctac actcggtgtc accggcggca acaagaaaag cggcctctat
    12961 gaagatggcg tgcgcaaaga ccgtctggac aacaacgata tgatcgacca gctggaagca
    13021 cgcattcgtg cgaaagccag tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt
    13081 gaaaaataat aacgtgatgg gaagcgcctc gcttcccgtg tatgattgaa cccgcatagc
    13141 tcccgaaaca ttgagggaag cgttgagggt tcatttttat attcagaaag agaataaacg
    13201 tggcaaaaaa cattcaagcc attcgcggca tgaacgatta cctgcctggc gaaacggcca
    13261 tctggcagcg cattgaaggc acactgaaaa acgtgctcgg cagctacggt tacagtgaaa
    13321 tccgcttgcc gattgtagag cagaccccgc tattcaaacg tgcgattggt gaagtcaccg
    13381 acgtggttga aaaagagatg tacacctttg aggatcgcaa tggcgacagc ctgactctgc
    13441 gccctgaagg gacggcgggc tgtgtacgcg ccggcatcga gcatggtctt ctgtacaatc
    13501 aggaacagcg tctgtggtat atcgggccga tgttccgtca cgagcgtccg cagaaagggc
    13561 gttatcgtca gttccatcag ttgggctgcg aagttttcgg tctgcaaggt ccggatatcg
    13621 acgctgaact gattatgctc accgcccgct ggtggcgcgc gctgggtatt tccgaacacg
    13681 taactcttga gctgaattct atcggttcgc tggaagcacg cgccaattac cgcgatgcgc
    13741 tggtggcatt ccttgagcag cataaagaaa agctggacga agactgcaaa cgccgcatgt
    13801 acactaaccc gctgcgcgtg ctggattcaa aaaatccgga agtgcaggcg cttctcaacg
    13861 acgctccggc attaggtgat tatctggacg aggaatctcg tgagcatttt gccggtctgt
    13921 gcaaactgct tgagagcgcg gggatcgctt acaccgtaaa ccagcgtctg gtgcgtggtc
    13981 tggattacta taaccgtacc gttttcgagt gggtgactaa cagtctcggc tcccagggca
    14041 ccgtgtgtgc aggcggtcgt tatgacggtc ttgtggaaca actgggcggt cgtgcaacac
    14101 cggctgtcgg ttttgcgatg ggcctcgaac gtcttgtatt gttagtacag gccgttaatc
    14161 cggaatttaa agccgatcct gttgtcgata tatacctggt ggcttcaggt gctgatacac
    14221 aatctgcggc tatggcatta gctgagcgtc tgcgtgatga attaccgggc gtgaaattga
    14281 tgaccaacca cggcggcggc aactttaaga aacagtttgc ccgtgctgat aaatggggtg
    14341 cccgcgttgc tgtggtgctg ggtgagtctg aagtggctaa cggcacagca gtagtgaagg
    14401 atttgcgctc tggtgagcaa acggcagttg cgcaggatag cgtagccgcg catttgcgca
    14461 cgttactggg ttaaggaagg agaaggacag cgtggaaatt tacgagaacg aaaacgacca
    14521 ggtagaagcg gttaaacgct tttttgctga aaatggcaaa gcactggctg ttggggtgat
    14581 tttgggcgtt ggcgcactga ttggctggcg ctactggaac agccatcagg ttgattctgc
    14641 acgctccgct tctcttgcct atcaaaatgc ggttaccgca gtgagcgaag gcaaaccgga
    14701 tagcatcccg gcggcggaaa aatttgctgc tgaaaataaa aatacttatg gtgcgctggc
    14761 ttctttggaa cttgcgcagc aatttgttga caaaaatgaa ctagagaaag ctgccgccca
    14821 gttacaacag gggctggcag acacgagcga tgaaaatctc aaagccgtga taaatctgcg
    14881 tcttgctcgc gttcaggtac agctcaagca ggctgatgcc gcgctgaaaa cccttgatac
    14941 catcaaaggt gaagggtggg ctgccattgt tgccgacctg cgtggtgaag cattgctgag
    15001 caaaggtgat aagcaaggtg cgcgtagtgc atgggaagca ggcgtgaaaa gcgatgttac
    15061 tccggcactg agcgaaatga tgcagatgaa aattaataat ttgtccatct gagagggacc
    15121 cgatgcaatt gcgtaaatta ctgctgccag gactgctttc cgttaccctt ttaagcggct
    15181 gttcgctgtt taacagcgaa gaagatgtgg taaagatgtc cccattgcca accgttgaaa
    15241 accagtttac gccgaccacg gcgtggagta cttccgttgg tagcggcatt ggcaacttct
    15301 attccaatct tcatccggca ctggcggaca acgttgtcta tgcagcggac cgcgctggtt
    15361 tggtaaaagc gctgaatgcg gatgatggca aagaaatctg gtctgtcagc ttggccgaga
    15421 aagatggctg gttctctaaa gagcctgcat tactttctgg cggtgtgacc gtgtctggtg
    15481 ggcatgtcta cattggcagc gaaaaggcgc aggtttacgc gctgaatacc agcgatggta
    15541 ctgtggcatg gcaaactaaa gtcgcgggtg aagcactttc gcgcccggtg gtcagcgacg
    15601 gtctggtgtt aatccacacc agtaacggtc agttacaagc gctgaacgaa gctgacggcg
    15661 ctgtcaaatg gacagttaac ctcgatatgc cttcgctctc tttgcgtggc gagtctgcgc
    15721 cggcaacggc ttttggtgcg gccgtcgtgg ggggcgataa tggtcgcgtc agcgcagtgc
    15781 tgatggaaca gggccagatg atttggcagc agcgtatttc ccaggcgacc ggttctaccg
    15841 aaattgaccg tctgagcgat gttgacacga ctcccgtcgt tgttaacggc gttgttttcg
    15901 cgctggccta taatggtaac ctgacggcgc ttgatctgcg cagtggtcag attatgtgga
    15961 aacgcgaact gggttcggtg aatgatttca tcgtcgacgg caatcgcatc tatctggtcg
    16021 atcaaaatga ccgggtgatg gcgttgacca ttgatggcgg cgttacgctg tgggcacaaa
    16081 gcgatctgct gcatcgcctg ctgacttctc cggtgctgta taatggcaac ctggtggtcg
    16141 gtgacagtga aggttatctg cactggatta acgtcgaaga tggtcgtttc gttgcccagc
    16201 aaaaagttga tagttccggt ttccagactg aaccggttgc cgctgacggc aaactgctga
    16261 tccaggcaaa agacggaacc gtgtactcta ttacacgtta atcgtctctg tcgttcactt
    16321 tgaaaacggc tcctggacag gggccgtttt cctgttttta acaacgacgc gaatatagcg
    16381 tgcgttgtct gatgatttat aaaaatgagg ctttaaacat ggtacctgtg gtcgcgcttg
    16441 tcgggcgccc taacgtagga aaatccacgt tatttaaccg tctaactcgc acccgagatg
    16501 cgctggttgc ggatttcccg ggtctgactc gtgaccgtaa gtacggtcgt gcggaaattg
    16561 aaggccgtga gtttatctgt attgataccg gcgggattga tggcacagaa gacggtgtag
    16621 aaacccgcat ggcggaacag tcgctgctgg cgattgaaga agcggacgtc gtactgttta
    16681 tggtggatgc gcgcgcgggc ctgatgccgg cagatgaagc gattgccaaa catctgcgct
    16741 cccgtgaaaa accgaccttc ctggtggcaa acaaaactga cggtctggat cccgatcagg
    16801 cagtggttga tttctactcg cttggtttag gtgaaatcta cccgatcgcc gcgtctcacg
    16861 gtcgtggcgt attaagtctg ctggagcatg tgctgctgcc gtggatggaa gatctcgcac
    16921 cgcaagagga agtcgacgaa gacgctgaat actgggcgca atttgaagcg gaagagaacg
    16981 gcgaagaaga agaggaagac gacttcgacc cgcaaagtct gccgatcaaa ctggcgattg
    17041 tgggtcgtcc gaacgtaggt aagtctacac tcactaaccg tattcttggt gaagagcgcg
    17101 ttgttgttta tgacatgcct ggcacgacgc gtgacagtat ctacattcca atggaacgcg
    17161 atggacgtga gtatgtgctc attgacaccg ctggcgtacg taaacgcggc aaaatcaccg
    17221 atgctgtaga gaaattctcc gtaatcaaaa cgttgcaggc cattgaagat gccaacgtgg
    17281 tgatgttagt gattgatgcg cgcgaaggta tttccgatca ggatctctcg ctgctgggct
    17341 ttattctcaa tagtgggcgc tcacttgtca ttgtggtgaa taagtgggat ggcctgagcc
    17401 aggaagtgaa agagcaggtg aaagaaacgc tggacttccg tctgggcttt atcgattttg
    17461 ctcgtgtgca ctttatctct gccttgcacg gcagtggtgt tggtaacttg tttgaatcag
    17521 tacgtgaagc gtatgacagc tccacccgtc gtgtggggac ctctatgctg acgcgcatca
    17581 tgacgatggc tgttgaagat caccaaccgc cgctggtacg cggtcgtcgt gtgaagctga
    17641 aatatgccca cgctggtggt tataacccgc cgattgtggt gattcacggt aatcaggtga
    17701 aagacctgcc tgattcctac aagcgctact tgatgaacta cttccgcaaa tcgctggacg
    17761 taatgggatc gccgattcgt attcagttca aagaagggga aaacccgtat gcgaacaagc
    17821 gtaacaccct gacgccaacc cagatgcgta aacgtaagcg tctgatgaag cacatcaaga
    17881 aaaataaata attatccgta agtattttat ctgccattcc ctctatgaat gtagagggat
    17941 ggctaccgga ggaattcata tggaactgca ttgtccacaa tgtcagcatg tgcttgatca
    18001 ggataacgac catgcgcgtt gcccatcctg cggggaaatt attgaaatga aagctctttg
    18061 tccagactgc catcaaccgt tgcaggtgtt aaaagcctgc ggtgcagtag attacttctg
    18121 tcagcacggg catggcttga tatcgaaaaa acgcgttgaa ttcgtgctgg cttaatgcac
    18181 ctttttacgc gattttttta ctggctggat gtttttaact tcactttcta tccagccgtc
    18241 ttccagacgt gtggttagca tttcacccgc tttaacttgc ttcacttttt tcagtacatt
    18301 gccgtcagta gcagtagtaa cgctgtatcc acgcgccagc gttgacagtg ggcttacggc
    18361 ttcgaggtgc gttactgcat taccgaaacg ttcacgcgtg gcgctaagct gtacgcgcag
    18421 ggtttctgct aaacgatatt ccagttgctg aatgcgcgtt tgcgcgcgat gaatcttcgg
    18481 ctgtggattt tgctgattca gccgctgtgt taaccgctgc tgctgttgcc cggtacgctt
    18541 aagttgattt tccagcgcaa agctcatccg cttttgcagg cgctcaagca tggtttgctg
    18601 gcgtgccagc cggagctgcg gatgctgttg ctgtaatcgg tgatggatct gcgtaaagcg
    18661 acgcgtgcgg ttggcgagat aataatccat cgccatctcc agccgttgac gggtcgattg
    18721 cacctggcgc agtaactctt gctgattacg gctcactact tcagcggcgg cagacggcgt
    18781 tggcgcacgc agatcggcaa caaaatcggc aatggtcaca tccgtctcat gcccgacggc
    18841 gctgacgacc ggaatgcggc tggcaaaaat cgcccgcgct acgcgttcgt cgttaaaact
    18901 ccataaatct tccagcgaac cgccgccgcg cccaacgatc aacacgtcgc actcattgcg
    18961 ctgattcgcc agttcaatgg cgcgaacaat ttgccccggt gcgtcatcgc cctgaacggc
    19021 ggtggggtag atgatcaccg gtagagaagg atcgcgacgt tttaacacat gcaaaatatc
    19081 atgtagcgca gcaccggttt ttgaggtgat cacaccaacg caatgcgcag gggagggaag
    19141 tggttttttg tattgctgat cgaacaaacc ttcagcctgc aacttcgctt tgagctgttc
    19201 gtacttctgt tgcagcagcc cttcaccggc cggctgcata ctctcaacga ttatctggta
    19261 gtcaccgcgc ggctcgtaga gcgtaatatt ggcgcgaact aaaacttgtt gtccatgctg
    19321 tggacggaag gtcacccggc ggttgctgtt gcggaacatc gcgcagcgta cctgggcggt
    19381 gtcgtctttg agtgtaaagt accagtgacc ggaagccggt tgtgtgaaat tagagatttc
    19441 gccgctgatc caaacctgtc ccatctcatg ctcaagcagc agacgaaccg tttgattcag
    19501 gcgactaacg gtaaaaattg caggggattg agaaggtaac atgtgagcga gatcaaattc
    19561 taaatcagca ggttattcag tcgatagtaa cccgcccttc ggggatagca agcatttttt
    19621 gcaaaaaggg gtagatgcaa tcggttacgc tctgtataat gccgcggcaa tatttattaa
    19681 ccactctggt cgagatattg cccatgctac gtatcgctaa agaagctctg acgtttgacg
    19741 acgttctcct cgttcctgct cattctaccg ttctgccgaa tactgctgac ctcagcaccc
    19801 agctgacgaa aactattcgt ctgaatatcc ctatgctttc cgcagcaatg gataccgtaa
    19861 cggaagcgcg cctggctatt gctctggctc aggaaggcgg tatcggcttt atccacaaaa
    19921 acatgtccat tgaacgccag gcagaagaag ttcgccgtgt gaaaaaacac gaatctggtg
    19981 tggtgactga tccgcagact gttctgccaa ccacgacgct gcgcgaagtg aaagaactga
    20041 ctgagcgtaa cggttttgcg ggctatccgg tcgttaccga agaaaacgaa ctggtgggta
    20101 ttatcaccgg tcgtgacgtg cgttttgtta ccgacctgaa ccagccggtt agcgtttaca
    20161 tgacgccgaa agagcgtctg gtcaccgtgc gtgaaggtga agcccgtgaa gtggtgctgg
    20221 caaaaatgca cgaaaaacgc gttgaaaaag cgctggtggt tgatgacgaa ttccacctga
    20281 tcggcatgat caccgtgaaa gacttccaga aagcggaacg taaaccgaac gcctgtaaag
    20341 acgagcaagg ccgtctgcgt gttggtgctg cagttggcgc aggtgcgggt aacgaagagc
    20401 gtgttgacgc gctggttgcc gctggcgttg acgttctgct gatcgactcc tcccacggtc
    20461 actcagaagg cgttttgcag cgtatccgtg aaacccgtgc taaatatccg gatctgcaaa
    20521 tcatcggcgg caacgtggca acagctgcag gtgcacgcgc tttggcagaa gctggttgca
    20581 gcgcggttaa agtcggtatc ggccctggtt ctatctgtac gactcgtatc gttactggcg
    20641 taggtgttcc gcagatcact gccgttgctg acgcagttga agccctggaa ggcaccggta
    20701 ttccggttat cgcagatggt ggtattcgct tctccggcga catcgccaaa gctatcgccg
    20761 ctggcgcaag cgcggtaatg gtgggttcca tgctggcggg tactgaagaa tctccgggtg
    20821 aaatcgaact ctaccagggc cgttcttaca aatcttaccg tggtatgggt tccctgggcg
    20881 cgatgtccaa aggttcctct gaccgttatt tccagagcga taacgctgcc gacaaactgg
    20941 tgccggaagg tatcgaaggt cgcgtagctt ataaaggtcg cctgaaagag atcattcacc
    21001 agcagatggg cggcctgcgc tcctgtatgg gtctgaccgg ctgtggtact atcgacgaac
    21061 tgcgtactaa agcggagttt gtacgtatca gcggtgcggg cattcaggaa agccacgttc
    21121 acgacgtgac cattactaaa gagtccccga actaccgtct gggctcctga ttctcttcgc
    21181 ccgacttcat gtcgggcgat ttatattatc tgtttcactt gcctcggaat aagcgtcaat
    21241 gacggaaaac attcataagc atcgcatcct cattctggac ttcggttctc agtacactca
    21301 actggttgcg cgccgcgtgc gtgagctggg tgtttactgc gaactgtggg cgtgggatgt
    21361 gacagaagca caaattcgtg acttcaatcc aagcggcatt attctttccg gcggcccgga
    21421 aagcaccacc gaagaaaaca gcccgcgtgc gccgcagtat gtctttgaag caggcgtacc
    21481 ggtattcggc gtttgctatg gcatgcaaac catggcaatg cagttgggcg gtcacgttga
    21541 agcctctaac gaacgtgaat ttggctacgc gcaggttgaa gtcgtaaacg acagcgcact
    21601 ggttcgcggt atcgaagatg cgctgaccgc agacggtaaa ccgctgctcg atgtctggat
    21661 gagccacggc gataaagtta ccgctatccc gtccgacttc gtcaccgtag ccagcaccga
    21721 aagctgcccg tttgccatta tggctaacga agaaaaacgc ttctatggcg tacagttcca
    21781 cccggaagtg actcacaccc gccagggtat gcgcatgctg gagcgttttg tgcgtgatat
    21841 ctgccagtgt gaagccctgt ggacgccagc gaaaattatc gacgatgctg tagctcgcat
    21901 ccgcgagcag gtaggcgacg ataaagtcat cctcggcctc tctggtggtg tggattcctc
    21961 cgtaaccgca atgctgctgc accgcgctat cggtaaaaac ctgacttgcg tattcgtcga
    22021 caacggcctg ctgcgtctca acgaagcaga gcaggttctg gatatgtttg gcgatcactt
    22081 tggtcttaac attgttcacg taccggcaga agatcgcttc ctgtcagcgc tggctggcga
    22141 aaacgatccg gaagcaaaac gtaaaatcat cgggcgcgtt ttcgttgaag tattcgatga
    22201 agaagcgctg aaactggaag acgtgaagtg gctggcgcag ggcaccatct accctgacgt
    22261 tatcgaatct gcggcttctg caaccggtaa agcacacgtc atcaaatctc accacaacgt
    22321 gggcggcctg ccgaaagaga tgaagatggg cctggttgaa ccgctgaaag agctgttcaa
    22381 agacgaagtg cgtaagattg gtctggagct gggcctgccg tacgacatgc tgtaccgtca
    22441 cccgttcccg ggaccaggcc ttggcgttcg tgtgctgggt gaagtgaaga aagagtactg
    22501 tgacctgctg cgccgtgctg acgccatctt cattgaagaa ctgcgtaaag cggacctgta
    22561 cgacaaagtc agccaggcgt tcactgtgtt cctgccggta cgttccgttg gcgtaatggg
    22621 cgatggtcgt aagtatgact gggttgtctc tctgcgtgct gtcgaaacca tcgactttat
    22681 gaccgcacac tgggcgcatc tgccgtacga tttcctcggt cgcgtttcca accgcattat
    22741 caatgaagtg aacggtattt cccgcgtggt gtatgacatc agcggcaagc cgccagctac
    22801 cattgagtgg gaatgatttt aatccctgca ctatgaatga acaaaaccct ctgttactac
    22861 agagggtttt ttatcttcaa gaatcatagg cttgaagtta ctaacatcga ttaattaaac
    22921 cagctgtccg atttgttctc ttctgctttg cccacgcttt tcatcagatc gcgaccgcct
    22981 tcagtcatat ttctgttggc gtcagcttca gattgcacca catcggtttg cgcagctttg
    23041 tgcttcagtt cctgatcgat aaattcgttt tctcgcttaa cgcgggcttc ttcttttgcc
    23101 agcgccagtt tttgtttctg aatctctaag ctgcgtagct catcttcata actttgatcg
    23161 cgttttttgt ccgcagaggc ttcggcgtcc agtttatcct gacgagcttt cttatttgcc
    23221 gctgccgttg ccgctctttt gttagcggcg gcctgggcat ttgcgcgacg ttgcttctct
    23281 tgctggattt ccctgttgcg ctccgcgacc cattcgtcat gctgcctttg ctcttcattt
    23341 ttaccttgct gttcagcttc tgctacagca gagagttgat cctgcaatga tgaggcgata
    23401 gccggatagc ataaggaggc caagatggcg caaagaaaaa ctttcttcat gactcctcct
    23461 gattattagc tcttttcagg acatttagta tttggctgaa tacgcgtttc gttatacgtt
    23521 ctggtaataa caacggctaa acctgtcgta aactggcact ctttacccac ctgggtagag
    23581 gtatacactt tggtgccttc cttatatgtt aaagaaacac cttccactaa ggttttatca
    23641 ttcaccatag aacccactgc cgcgcctaca gctccgccgc caactgcccc tgccgtcgtt
    23701 ccggaattgc tgccagaacc gacgttgtgg ccgataacac cgccagcgac tgcgccaata
    23761 agcgcgccga aggcttgtgc gttccgttta ttttgggagt tgtctacggc aacttttgcg
    23821 ggaagaatgg aaataatatt aacggtttta gtttcttgtt tggtattcag ttgatcggtt
    23881 tgataaacat cggcggcatg atcgtcagca tttgactggc atcctgccag agtgaatgac
    23941 gctaacattg ccacaggcag aagacatttt ttaaatttca tcactattcc ttgttataca
    24001 cattacgaaa aataagacat ttaagatcat caaaagagga aagtaaatgt attgcgtata
    24061 aagggataga ttaaatttat taaaattcac gcagggaata atttataaaa atatatcatt
    24121 acaacatata tattaatttt atatttattt cgcagccaaa caggataaac atcagtcagg
    24181 acaaagacgt aaaatttaag atgaaagaat atctgccgct ttgataattc caaagagcaa
    24241 aagcatccga cagcgaagtg ccgttaggtt ctgtgaaagt ggagaaggta accgctaacg
    24301 ttgtaccggt gattcgacgg agagtgtgga ttgctgagct tcttttttgc tctgatggtg
    24361 ctgccaggca ccgacggacg agtagataaa acggccaaag aagaagataa agctgatgag
    24421 cagtacgata cgggtcatgc gactgttaaa tcggtgtcgt tttcgcatac tggttgcctg
    24481 actcacaaaa ggttccttga agtatgtccc acgctgtgga cggtacttac attaaggcac
    24541 aacaggacaa aatggtcaat tcttcgttat gtaaaaaagc gtcagtggat acatatttta
    24601 atgttatgga agttaattta attatttaca atgatgatgt aataatgatg aattaacatg
    24661 ataaacgtaa taattcatta atcagggtta ttttgtttga tatatatcaa ttggaacttt
    24721 catatgacac ttagaatcaa tgctctctat agtgatgaat aatcatcatt cgaagtcagg
    24781 tgggatgcct gtctgaatac acctccttca ggatgtgggg gattctgctg agcatctatg
    24841 aaactgaatg caacttatat aaaaatacgt gataaatggt gggggcttcc gctgttcctg
    24901 ccttctttaa tcttgcccat tttcgcccac attaatactt tcgcgcatat ttcttccggt
    24961 gaggtttttc tcttttatct gcctttggca ctgatgatca gcatgatgat gtttttcagc
    25021 tgggcggcat tgccagggat caccttaggg atttttgtcc gcaaatatgc agagctgggt
    25081 ttttacgaaa cgctctcatt aacggctaat tttattatca ttatcattct ctgttggggc
    25141 ggttacaggg tctttactcc ccggcgtaac aacgtttcac atggtgatac ccgtttaatt
    25201 tcccagcgta tattctggca gatagtgttt cctgcaacgc tgtttctgat acttttccag
    25261 tttgctgcat ttgtaggatt gctggcgagc agagaaaatc tggtcggtgt catgcctttt
    25321 aacctcggga ctttaatcaa ttatcaggcc ttactggtgg gtaatctgat cggtgtcccg
    25381 ctgtgctact tcatcattcg ggtagtgcga aatccatttt atttacgtag ctattattcg
    25441 caattaaaac agcaggttga tgccaaagtc accaaaaaag agttcgcgct ctggctactg
    25501 gcattaggtg ctttattgtt gctgttatgc atgccgttaa atgaaaaaag cacaattttt
    25561 agcaccaatt ataccttgtc attattgctg cccctgatga tgtggggagc gatgcgctat
    25621 ggttataagc tgatttcgct gctctgggcg gtcgtgttga tgatcagcat ccacagctat
    25681 caaaattaca ttcccattta tcctggctat accacgcagc tgaccataac ctcctccagt
    25741 tatctggtat tctcttttat tgtcaattat atggctgtac tggcaactcg tcagcgagcg
    25801 gtagtcagac gcattcagcg gcttgcgtat gtggacccgg tggttcatct gccaaatgtt
    25861 cgcgccctga atcgcgcgtt gcgtgatgcc ccctggtctg cgctttgtta tttacgcatc
    25921 cctggcatgg aaatgctggt taagaactat ggcatcatgc tgcggattca atacaagcaa
    25981 aaactttctc actggctgtc acccttgctg gaaccgggtg aagatgttta tcagctttcg
    26041 ggtaacgatc tcgcgctgcg gctgaataca gaatcgcacc aggagcgcat taccgcactg
    26101 gatagccatc tcaagcaatt tcgtttcttt tgggatggca tgccgatgca accgcagatt
    26161 ggcgtcagtt actgctatgt gcgctcgcca gtgaatcata tctacctgct gctgggagag
    26221 ctaaatacgg tcgccgaact ttccatcgtg accaacgccc cggaaaatat gcagcgtcgc
    26281 ggggcaatgt atttgcaacg cgaattgaaa gataaagtcg cgatgatgaa tcgactacag
    26341 caggcgctgg aacacaacca ttttttcctg atggcccagc cgattaccgg tatgcgtggt
    26401 gatgtttacc atgaaattct tctgcgcatg aaaggtgaga atgatgaact gatcggcccc
    26461 gatagcttct tgccggtcgc gcacgaattt ggtttatcgt cgagtatcga catgtgggtc
    26521 attgagcata cgctgcaatt tatggctgaa aacagagcga agatgcccgc tcaccgtttt
    26581 gctattaatc tgtctccaac ctcggtatgt caggctcgtt ttcctgttga agtcagtcag
    26641 ttgctggata aatatcagat tgaagcgtgg caacttattt ttgaagtcac cgaaagtaat
    26701 gctctgacca atgttaagca ggcgcaaatc accttgcagc atcttcagga attaggctgc
    26761 cagattgcga ttgatgattt cggcaccggc tacgccagct atgcgcggct taaaaatgtg
    26821 aatgccgatc tgcttaaaat tgacggcagt tttatccgca atattgtgtc aaatagtctg
    26881 gattatcaga tagtggcatc gatttgccac ctggcgcgaa tgaagaaaat gcgggtagtg
    26941 gcagagtacg ttgaaaacga agagatccgc gaggcggtgc tctctttggg gatcgattat
    27001 atgcagggtt atcttattgg taagccgcaa ccgttaattg atacgctgaa tgaaatcgaa
    27061 cccattcgcg aaagtgcctg aataatgcgg gccgacattt ctcgtcggcc cgcaaagcat
    27121 taagcggcga tttcaggtgt actttcttct tcaattttca accgccagcc agccacgcct
    27181 tcccagtatt cttgctcctt ttccagatca agcagtacca gcgcattctg actaaaccag
    27241 tcatgcggga aacgcagtgt ccagtgactg tcatcagtaa tcagtgtcaa tgttggcggt
    27301 gtggtggttg cctgacgttg attgtttagt aatacgccaa ggcgcaatag ctgtatcagt
    27361 ggcaggaact gtttcttctt aaacaaggta aagcgcggca gatcgtcgag cttaatcgct
    27421 ttacggtgat agcgcaccag tgtcgccatc atcagctgct gttcctgatt aaaacccggc
    27481 aagtcactgt tttgcagaat ataagcggag tggcgatgca aaccgctgtg gttgatattc
    27541 aacccgacct catgcagcat ggcggcccat cgcagtagcg cctccagttg cggatgcgcc
    27601 agcttcggtt gctgttcccg ccactgttcg tacatttgca tagtggtatc cagcacccgt
    27661 cgggcctgct cgctgtcgat gtgatactgg ttggcgaggc tgctggcggt gcgactacgc
    27721 acatcctgat gacggaaacg tccttccatt tcatacagta cgccttcgcg taaagccccg
    27781 tcagaaaggc gcagttcacg gatggctaaa gcatcaaaca caccgcataa aatcgccagt
    27841 ccaggaacga agactgtttt ccgctcttcg gaaagacccg gtaaactcag cgatgcgaaa
    27901 ttacggtgac gtaaaacttc ttttaccagt ttttccagac gttccggggt aattatcccg
    27961 tctttctcgc ccatttccat taacacttca tgggcggctt ttatggtacc ggaagcgccc
    28021 attgccacgt tccagccctg aatacggaat tgccaggtta aagtttccag tttttgtgct
    28081 gccgccatac gagcgcgctg aaaattctct ttattgatga ccccgccagg gaagtaaagc
    28141 tgggcaaagc tgacacaacc catccggcgg ctttcaacga gaatcggttc gaaattttca
    28201 ccaatcacca gttccgtaga tccgccgcca atatcaataa ccagtttgcg acctttctcc
    28261 ggttgggtat gttccacgcc cataaaaatc agacgggctt cttcattacc ggaaataatt
    28321 tcaatcgggt agggaatgac cttttccgcg cgtttcagaa agtcagtggc gttcagcgcc
    28381 tgacgcagcg tatgggtgcc gactatacag acgctggcag gagaaaaccc ttgtagccgt
    28441 tcggcaaaca gcgacagaca gttcaaaccg cgcgtcattg cctcttcact caacatatta
    28501 tctggcccca ggccgtccgc cagatgcacc cgctgtttca ggcggccaat aatctgcatg
    28561 gcaccatcta ccacacgggc tatgaccatg tgaaaactgt ttgaaccaag atcgaccgca
    28621 gcaaactcct gcggacgagg ggatttatcg tgtattggca tagggttatt caggttgttc
    28681 gagtgatttg atgtagtcgt aaatcgccaa ctgcgcccgt actttgcggc gattgccgcg
    28741 gggaacgtag cgattactga gttctttatc gatataacgt gctttgaccg tatcgctgaa
    28801 caatatgtcg atgatgtcca gcacccgctg cttcaggcgc ggatcgagca gcggcgtcgc
    28861 cacttcaata cgataatcaa tattgcgcgt catccagtcg gcggaagaaa ggtagacctt
    28921 tttatcgccg ccattttcaa aaatataaac ccggtcatgt tcaaggtaac ggtcaacaat
    28981 actgatggca cgaatgttgt cgctaatgcc ttccagattg gggatcagcg aacacattcc
    29041 gcgaaccagc agattaaccg gtacgccgga gctggaggcc gcatacagac gatcaaccag
    29101 gcctttatcg acaaggttat ttagcttcag ggtgataccg ctgggcagcc cttgctgcgc
    29161 gttggcgatt tcgcggtcta ccatttcata taacagacgg cgagagtttt gcggtgacac
    29221 cattaaataa tcaaatgtca ccgggcggta tgggttttca ataaagttaa atacccgccg
    29281 tacttcgttg gtgatacgcg catcggcggt cagcaatgaa tagtcagtat aaagacgcgc
    29341 ggttttttcg ttaaagttcc cggtcccgat gtgtgcgtaa cgcaccactt caccgttttc
    29401 ttttcgtgaa atcaggaaca gtttggcgtg aattttcagc cccggcgcag agaagataac
    29461 gtgcacgcct gcttcggtca ggcgcttcgc ccagtgaatg ttagcttctt cgtcgaaacg
    29521 cgcctgtaac tcaaccacca cggtgacttt cttaccgtta tgtgcggcgt ggatcatcga
    29581 gtcgatgatg cgtgaatctt tcgccacgcg gtaaatgtta attttaatcg ccagtacgct
    29641 cgggtcgaac gaagcctgac gcagcagttc cagcacatgc tcaaaggtgt gataaggata
    29701 atagagcaac acatcgcgtt cgcgaatggc atcaaaacca ttgcggaact gggctttatc
    29761 aaaccaaata tggcgtaaac gcggcagtgg tttgttcacc agattggctt tgccgacatt
    29821 ggggaaatta ataaagtctt taaaattatg ataacgaccg ccggggacga tggagtcgta
    29881 gcgggaaata gtcagttttt cgcgtaacac ttcaaccagc gcattgggca tatcgcgctg
    29941 ataaacaaaa cgcaccggct cagcagttaa acgctgcttg agactggaag acatcaactc
    30001 catcaggctg gcttccatct catgcactaa atcgtattcg gcatcgcggg tcatcttcat
    30061 tgaataggca ttcagcgcgt cataatcaaa gaagcctttg aaaatatcat caaggcagta
    30121 acgcagaatg ttatccagaa gaatcatcgg cttgcgtcga cgcggcgctt ctggcggtaa
    30181 attcacaaag cgcggcactt tatctgatgg gatctccagc agcgcgtaac ggatggtatc
    30241 gccacggata atttccaccg ccagataggt gtaatcatct ttcaggaact gcactaagtc
    30301 agtgtcagga ttgattaaaa tcggcgtaat gtgctgacgc agatactgct taaaataatg
    30361 acgcagccag ttttgttgat tgacggagag ctggcgttca ttaatcagga agatctggtt
    30421 acgcgccatc tccagcagca gctcgttgta gaggccgtcg aattcctgat cggctttcag
    30481 cacccgggac tgaattttgc ccagtaaatg gcgggaatga gagttggagc cttgttcttc
    30541 gctaataatg atgcgtcgct tcagttcagc gaagcggact ttatagaact catcaaggtt
    30601 attggaatag atccccagga aacgcatcct ttcaatcagc gggttagatt tgtccgccgc
    30661 ttcctgaagc acgcgttcat tgaacgataa ccaactgagc tctttttcga tgtatagctt
    30721 ttcctgaccc attaccactt ttactccgtt ttattcacgg gacactgcct ggatattatg
    30781 gcgagcaata ttggcagatg tccaactatg ccagaaaagg ggaaaaaaac tgctggagct
    30841 tgcgatttac aggtgaattg aaaaacgcca gcggcagagc tggcgcttta actacggggc
    30901 attactcgtc ggcagcgtag ccctgcggcg gcagacgttg accatccagc cacgcggcgt
    30961 tttcgtgcat tttcagacga ccatcggcaa accagctaat caccagtgga taaatagcgt
    31021 gttcctgggt ttgcacgcgg gcggtgatgt catcttccgt atcaccagca aataccggga
    31081 ctttcgcctg taaaataacc gggccaccgt ccagttcatc ggtgacgaaa tgcaccgatg
    31141 taccgtgctc ttcatcacca ttttccagcg cctgacgatg ggtgtgtaat ccgggatatt
    31201 tcggcagcag agaagggtga atgttcagca aacgcccggc atagtgggag acaaacgccg
    31261 ggctgagaat gcgcataaaa ccagccagca cgaccacatc gggtgcgtac atgtcgattt
    31321 catgaatcaa ctcccggtca taggcttcac gactgtcaaa cgcgctggcg atgagcgtat
    31381 gcgttgcaat acccgcctgg cgggcgcgtt caaggccgaa cgcgtcggcc ttattgctga
    31441 aaactgcccg tacggtgcct ttaattttgt tagttttaca ggcgtcaata attgcctgta
    31501 aattacttcc gttgccggaa ataagcacca caatattcat tattcgataa ccacgcgttg
    31561 ttcggaatca gaggctttga tgataccgat tttccacgcg ttttcaccgt tggagtttag
    31621 cagggcgagg gctttgtcca cttccggagc aggcagggcg ataatcatcc cgacgccgca
    31681 gttgaaggtg cgatacattt catggcgctc aacgttacct gccgtttgca gccagttgaa
    31741 cacttccggc cactgccagg aagattcatc aatcactgcc tgagtattat ctggcaatac
    31801 gcgtggaatg ttttcccaga agccgccgcc ggtcaggtgc gcaatggcat gcacatcgac
    31861 cttttcaatc aactccagca ctgacttcac gtaaatgcgg gtcggtgcca gcagatgatc
    31921 ggctaatggc ttaccatcaa gttcggtggt ttgcggatca caaccgctga cttcaagaat
    31981 tttgcgcacc agcgaatagc cgttcgagtg cggaccgctg gaaccgagtg caatcagcac
    32041 atcgccgtcg ctgactttag agccgtcgat gatttctgat ttttctacca cgccaacgca
    32101 gaaacccgcg acatcgtaat cctcaccgtg atacatcccc ggcatttctg ccgtttcgcc
    32161 acccaccagt gaacagcccg attgcagaca tccttccgcg ataccgctga tcaccgctga
    32221 agcggtatca acatccagtt ttccggttgc gtaatagtcg aggaaaaaca gcggctctgc
    32281 accttgcacc accaggtcat taacgcacat ggcgaccaga tcaataccaa tggtgtcgtg
    32341 acgttttaag tccatcgcca gacgcagctt ggtacctacg ccgtcagtac cggaaaccag
    32401 cacaggttca cgatattttt gcggcaatgc acacagcgca ccgaagccgc ccagaccgcc
    32461 catcacttcc ggacgacgcg ttttcttcac tacgcctttg attcttccaa ccagagcatt
    32521 acccgcgtca atatcaacac cggcatcttt gtagctaaga gaggttttat cggtcactgc
    32581 ttgggtcccc acgcgttact tgcggtagaa aaataaaatt cggcgcaatt ctaacaggga
    32641 aagcaaacgt ttgcgagact gctttacaca acctttttgc acgtcttttg cccaggcacg
    32701 cggcgaaaga agacttgtgc cagggtaaag gttagttttc ggatggaata atcttctttc
    32761 ataaccattt gaatataaaa taactttatc tcaaaccgtt atcattttga ctaaagtcaa
    32821 cgaaaagaat attgccgcct tgaagaaagg aggtataatc cgtcgatttt ttttgtggct
    32881 gcccctcaaa ggagaaagag tatgaagatc gtggaagtca aacacccact cgtcaaacac
    32941 aagctgggac tgatgcgtga gcaagatatc agcaccaagc gctttcgcga actcgcttcc
    33001 gaagtgggta gcctgctgac ttacgaagcg accgccgacc tcgaaacgga aaaagtaact
    33061 atcgaaggct ggaacggccc ggtagaaatc gaccagatca aaggtaagaa aattaccgtt
    33121 gtgccaattc tgcgtgcggg tcttggtatg atggacggtg tgctggaaaa cgttccgagc
    33181 gcgcgcatca gcgttgtcgg tatgtaccgt aatgaagaaa cgctggagcc ggtaccgtac
    33241 ttccagaaac tggtttctaa catcgatgag cgtatggcgc tgatcgttga cccaatgctg
    33301 gcaaccggtg gttccgttat cgcgaccatc gacctgctga aaaaagcggg ctgcagcagc
    33361 atcaaagttc tggtgctggt cgctgcgcca gaaggtatcg ctgcgctgga aaaagcgcac
    33421 ccggacgtcg aactgtatac cgcatcgatt gatcagggac tgaacgagca cggatacatt
    33481 attccgggcc tcggcgatgc cggtgacaaa atctttggta cgaaataaag aataaaaata
    33541 attaaagccg actttaagag tcggcttttt tttgagtaaa gcgcctataa cacataatac
    33601 agaggataat actatgacgc gccgtgctat cggggtgagt gaaagaccgc cacttttaca
    33661 gacaatcccg cttagtttgc aacatttgtt cgccatgttt ggtgcaaccg tcctggtgcc
    33721 cgtcttattt catattaacc cggcgactgt actgttattt aacggtattg gaacgctgct
    33781 gtatctcttc atctgtaaag ggaaaattcc ggcttatctt ggttccagct ttgcctttat
    33841 ttcaccggta ttgttactgt tgccgttagg gtatgaagtc gcgctgggcg gctttattat
    33901 gtgcggcgtg ctgttctgcc tggtttcttt tatcgtgaag aaagcgggga ccggctggct
    33961 ggacgtgctg tttccacctg cggcaatggg cgcaatcgtt gccgtcatcg gtctggagct
    34021 ggcgggcgta gctgccggta tggcgggttt actcccggct gaagggcaaa cgccagactc
    34081 caaaaccatc atcatctcta ttaccaccct ggcggtcacg gttttaggtt ccgtgctgtt
    34141 tcgtggtttc ctggcaatta tcccgatttt aattggcgtg ctggtgggct acgcgctctc
    34201 tttcgcaatg ggaattgtcg ataccacgcc gattattaat gctcactggt ttgcgctgcc
    34261 aaccctctat acgccgcgct tcgagtggtt tgccattctg actattctgc cagcggcgtt
    34321 agtggttatt gccgaacacg tagggcacct ggtagtaacg gctaatatcg tcaaaaaaga
    34381 tctgctgcgc gatccaggtc tgcaccgttc gatgtttgct aatggcttgt cgaccgtgat
    34441 ctccggcttc tttggctcta cgccaaatac cacttacgga gaaaacattg gcgtgatggc
    34501 gatcacccgt gtttacagta cctgggttat cggcggggcg gcgattttcg ctatcctgct
    34561 ttcctgcgtc ggtaaactgg ctgccgctat ccagatgatc ccattgccgg tgatgggcgg
    34621 cgtttcgctg ctgctttatg gtgtcatcgg tgcttccggt attcgtgttt tgatcgaatc
    34681 gaaagtggat tacaacaaag cacagaacct gatcctgact tccgtgattt tgatcatcgg
    34741 cgtcagtggc gcgaaggtaa acatcggcgc ggcggagctg aaaggtatgg cgctggcgac
    34801 catcgtcggt atcggcttaa gtttgatttt caaactgatc agtgtgctgc gccctgaaga
    34861 agtggtgctg gacgcagaag acgcggatat aacagacaaa taatgtaccg accgggcagt
    34921 gttgctgccc ggttcaaaca tcatgggatt ctgtggtaaa cttctcgcga ttttgtgaaa
    34981 tcctggttga ggtatctctg aacacaccgg cacagctctc tttgccactt tatcttcctg
    35041 acgacgaaac ctttgcaagt ttctggccgg gggataactc ctctttactg gccgcgctgc
    35101 aaaacgtgct gcgtcaggaa catagcggtt acatctatct ctgggcacgc gaaggcgcgg
    35161 ggcgcagcca tctgctgcac gcggcttgcg cggaattgtc gcagcgtggc gatgcggtgg
    35221 gctatgtccc gctggataaa cgcacctggt ttgttccgga agtgctcgac ggtatggagc
    35281 atttgtcact ggtctgtatc gacaacattg agtgtattgc cggcgatgag ttgtgggaga
    35341 tggcgatttt cgatctctac aatcgaattc tggaatcggg caaaacacgg ttgttgatca
    35401 ccggcgatcg tccaccgcgg cagttgaatc tgggattacc ggatctcgcg tcgcgactcg
    35461 actgggggca gatctacaaa ttgcagccac tttctgatga agataagttg caggcgctac
    35521 agttacgcgc gcgtttgcgt ggttttgaac tgccggaaga tgtggggcgt ttcttgctga
    35581 agcggctcga cagagaaatg cgcacgctat ttatgacgtt ggatcagttg gatcgtgcgt
    35641 cgattaccgc gcaacgtaag ctgaccattc cgtttgtgaa agaaattctg aagttgtaga
    35701 taaggtgttt attgtcggat gcggcgcgaa cgccttatcc gaactacgcg aagttaatcc
    35761 atagtattcg taggccggat aagacgtggc aagcgtcgca tccggcaatg tgcttcaact
    35821 agcgatggag cacatcttaa cccacgattt ccagtacctg ctccggtgga cgaccaatcc
    35881 gcgctttgcc attcgcgacc acaatcgggc gctccatcag cttcggatta tccaccattg
    35941 cctgaatcag cgcctcttcg ctaagcgaac tgtcagccag gttcagttct ttataaagat
    36001 cctctttctg gcgcatcaat tcacgggcgc tattcatccc cagcattttc agcaaatcgc
    36061 gcagcgttgc cgcatcggcg ggtgtctcaa ggtagagtac cacttctggc tccacgccgt
    36121 tttctttcag caggttcagc gtttcccggc tctttgaaca gcgtgggtta tggtagattt
    36181 ttacctgttt ggtcattgtt cttcctttaa tgcgaattac atcttggtat aaggcttaaa
    36241 gcgttcctgc agctggcgca actggtcgat gcgcgcatcg taacgcgctt gttgcaggct
    36301 gcctaatttc acctgcgaac tggcgctact caacagcgaa atggcctgat cgagtcgtcc
    36361 ggcgagcgca taaccttctg ctcgcgcagc cagctcctga tcgcggttat ttagcgcggc
    36421 ttccgcctgt gccagtaaat cccagccgtt gctgtcatct ttattattaa aggtgtagcg
    36481 attcagaata ttggccgctt cttgtggttg accgccttgt agataagcgt tcgccaggtt
    36541 gagctgcaac accggattgg tgcgcaaatc acgggcattt ttcaggcgat tgattgcctc
    36601 attggctttg ttttgcccaa gatcgatatc ggtagccaga tcgagatacc atgcgttgcc
    36661 aggttctgcc gccagtaacg gttgcagcgt tttacgcgcc tcgtcgtatt tattggcttc
    36721 catcgcctgt aaagcacgac catattgcgc cgctcgttgc tgacgaacgt ttcctttcgc
    36781 ccattcatcc agcaaatcac tggtgagctg gttacgtccg gaattataca tccccagtgt
    36841 gcgcgctttc gccagataga aatcttccga cgactgcacc accatcggtc gcatctgatt
    36901 agcacggttg cgggcatctg ccagacgact ttccggcaac gggtgagtca ataaaatttc
    36961 cggcgggcgc gaggagtaac gcgcctgatc gagtaatttt tcgaggaagg ttggcatcgc
    37021 ctgcggatcg aatcccgagc gttgcagcac ctgaatacca atgcgatccg cttcctgttc
    37081 attttgctgg gtgaagctga tcatcccctg gcgcgttccc gccagtgtac cggtcagcgc
    37141 tgccatcccc gcctgcggac tggccatcgc cagtaaaata gaacctaacg cgccgaccca
    37201 ggtcagcggc gcgctgcgct gttgatcttc catcgctcgc gccaggtgac gttgggtgac
    37261 gtgggagatt tcatgcgcca ttactgaagc cagttggctt tcgttatcgg aataacggaa
    37321 cagggcagag tgcagtacca cgttgccgcc aaagaaagca aaggcgttaa tttcgtcgtt
    37381 gttgatcaga aaaaaatgaa acggtgtctt aaccgaattg gcatgcgaaa ccagacgcat
    37441 tcctagtgaa ttaatgtact gcgttaatag tggatcatta attaacggcg cgctgccgcg
    37501 tagctggcgg acataatagt cgcccatctg catttcctga ccaatggaaa gcgtgcttcc
    37561 tgcggaggtt cccatatccg gcaaggtgtc tgcactatcg gcaaacgccg gggctacctg
    37621 accaatggtc atggcagcaa tgagggttgc aaccaggttt tttttcaact gcctgaacat
    37681 aacctctatc ctgtatttct ggagatagtc atttgaccga tactgtgaaa tatcgttccc
    37741 accgcgccgc ctttgagtgt aacggcgaag attagtccac tcaaatgact tttcagtttc
    37801 ctgaatccgt cataaaaagc gaagtctttt ttgtgacatt tcgatacaat tgcggatcgc
    37861 aatcccgctc ttgaggttca gggaaggtct ttatgctcga aatgttgatg caatggtatc
    37921 gccgccgttt tagcgacccg gaagcgattg ccttgctggt tattttagtt gccggatttg
    37981 gcattatctt tttctttagt ggcctgcttg ctccgctgtt ggtggctatt gtgctggcct
    38041 atttgttgga atggccaacc gtgcgcctgc aatctattgg ctgctcccgc cgctgggcga
    38101 cgtcgattgt attggtggtt ttcgtcggta tattgctatt gatggcgttc gtggtactgc
    38161 ctatcgcctg gcagcagggc atctacttaa ttcgcgatat gccggggatg ctcaataagc
    38221 tttctgactt tgccgccacg ttgccgcgcc gctatccggc gttgatggat gcgggcatta
    38281 ttgatgcaat ggccgaaaat atgcgcagtc ggatgctgac catgggcgat tcggtggtga
    38341 aaatttccct cgcctcgctg gtcggtttgc tgaccattgc cgtctatctg gtgctggtac
    38401 cattgatggt cttcttcctg ctgaaagaca aagagcagat gctgaacgcc gttcgccggg
    38461 tgctgccgcg taaccgtgga ctggcaggac aggtgtggaa ggagatgaat caacaaatca
    38521 ccaactatat ccgcggcaaa gtgctggaga tgatcgtggt ggggattgcc acctggctgg
    38581 gattcttact cttcgggctg aactattcgc tgctgctggc ggtgctggtc ggcttctcgg
    38641 tacttattcc gtacattggc gcatttgtgg tgaccattcc ggtggttggc gtggcgctat
    38701 tccagtttgg tgcaggcacg gaattctgga gctgcttcgc ggtgtatctg attattcagg
    38761 cgctggacgg caacctgtta gtaccggtgt tgttctccga agcggttaac ctgcatccgc
    38821 tggtgattat tttatcggtg gtgatcttcg gtggtttgtg gggattctgg ggcgtattct
    38881 tcgccattcc gttggcgacg ctgatcaaag ccgtgatcca cgcctggccc gatgggcaaa
    38941 tcgcgcaaga ataatcttca gcccgacgaa ctgtttcgcc gggctgattt ttcagggttc
    39001 ctgacgtaaa taaatagccc gataacaaat actcaccagc actgcaccgc cgataatatt
    39061 acccagcatc accgggagca gattggcggt aataaaatgg ctgaccgtca gtgccggaaa
    39121 attgtctgcg ctactgtgcg ccagttgcca gaaggaggta ggggcgaaat ggcgaatggc
    39181 aatggcgaac ggaatcacaa acaaattggc gatacagtgc tcaaagccac tggcgacaaa
    39241 cagggtgatg ggcaaaatca tggcgacgat tttgtcgcat aacgaacgcc cgcaataact
    39301 catccacagc gccaggcaaa ccatcagatt gcacataatg ccgaggctga cagattcagt
    39361 aaatgtatga tgcattttgc cctcggcgca gtgtaaaacc gcgactcccc acatcgcgtt
    39421 ttcactcatc accagcccgg aaaaccagat taacaaactg aaacaggcaa tacctgccag
    39481 attaccgcag gccaccagaa gtgcgttaat cagccaagtt cgccaactaa taacgccccg
    39541 actttttgcc attaccgtca ttaccgacga ggtgaacagg ttggtgccgc aaaccgccag
    39601 caaaataaag ccaagtgtaa agcaaaggcc gcccaccaga tgggttaatg cctgtgaaga
    39661 cggggcatcg gcgataactg aaaggtaaaa agtaaatccg atcgccataa acacgccagc
    39721 ggacatcgcc attaaaaaga ccgacacttt actgcgggca attttatggg cggcaatccg
    39781 ttcagcgatt gccgcttttc tggcgctcaa ctgcaagtcg aaagagagtt tgtttctcat
    39841 gcatactcct gcaaaagcag attacaacac ctcgcgaacc gagattccca gacgctgcat
    39901 tcgtgacagc agcgtggtgc gcttcatccc taatcgtgtc gccgctccac ggggcccggc
    39961 aacaatgcca ttggtttctc gcaatacctg aacaatgcgc tgacgttctt cttcgtcatt
    40021 ctctggcgtc gtcgggtgca gcaactgcgc cattgaactg cgaagcgctg aatcttcatt
    40081 tagcgtcggc agtaaacggc tttgtcggac attaagatgt aaattcagac tgttaccgcg
    40141 agtcagcagt accgctcgct caatcacgtt ttccagctcg cgcacgttgc ccggccagtc
    40201 ccacgacatc aactggcgta gcgcctcggt cgggatggcg tcaattgaac gattcatatg
    40261 gcgcgccatt ttttgcgtga aatgctttgc taaaagaggg atatcttccg gacggtcgcg
    40321 cagcggcggc aattccagtg ggaagacatt caggcgataa aagagatcgc tgcgaaactg
    40381 gcgatcttca accatttgcc acaaatcacg gttggtggcg gcaatgactc tgacatttac
    40441 cgggatcgtt ctactcccgc cgagacgctc aatctcccgt tcctgcaata cgcgcagcag
    40501 tttaggctga agttctaacg gcagatcgcc aatttcatcg agaaacaacg tgccgccatc
    40561 ggcaatttca aaacgaccac gatgtgtatt aatcgcacca gtaaacgccc ctttgtcatg
    40621 accgaataac tcactttcca gaagactggc ggggatggca gcgcagttga ttttgaccag
    40681 cggcttgtcg cgtcgcgggc taagttgatg gatcgctctg gcgatcacct ctttgccggt
    40741 tccggtttca ccgcaaatca acaccgtact gtcgctcttc gccacaatat ctacctgctg
    40801 gagcaggtct tccattgcct ggctttgata gataatgtca ccgacgccca gattcgaaag
    40861 gagctgctcg ctaagctggt ggttttcttg ctgcaaactt tcctgcaaat cggtcatgct
    40921 gcgccaggca tcggcatcgg cattgccaac ggcaatagcg atgcgatcgg ctatgtgttg
    40981 tagtagctgg cagttttcct cactaaagag agtggaagag gtatgcgcca gcaacaatgc
    41041 acccggtgta tggttgccaa aggtaagcgg tatcaggagc gcagattcgc agccgttaga
    41101 tgcaagtaag agtaataacg ggtcgcgttg ccagagaaac agatcatctg cacgatgcgt
    41161 caaggtcggt tgtcgggttt gtagcgtttg tgtcagcaat acactgtcgc caggcatatt
    41221 gcgtggcaga cacgcacaat gtgaggcaga aagatcgctg caccacaggc tgaacttctc
    41281 gttctttcga tgatcgccca gtaccatact gactgaagcc agaccgaaaa aatgatggat
    41341 ctcacgggcg acgtcagcga tcagatcgtc gagatcaaga tgtgacagca ctgcattagt
    41401 gatatccacc agtacccgat gatgatcgcg atctttctcc agcaaacgca gcgtcacctg
    41461 ctgttcttca tcattgagcc atgattgcag aaccggggtg ataatgcgtg ccagccagtt
    41521 aaacagtgtg caatcctggg gagtaaaacg gcagccgtgg gtacgaataa agcgtagctc
    41581 cgccattcca ctactgccga gaaggacggc gtgacgcgaa taatcgacca tctcgccctg
    41641 atactctgtt gttgccgtca catcgctgcc atcatcatgg tagcggtaat agaagttgct
    41701 tttgagaaca tgcagttcaa taagttgtac cgggctgaac ggcggcggca gcgggatgaa
    41761 ggcgcgtaat aaagacgcct taccgtgttt ctgcgctaac cgctccgcga gcattccgtt
    41821 taccgcttca attgttattc cttgcggcgg ggcaaacatc gcctcgtctg acatagccat
    41881 caacagtcct tattaatgag aaatccttct cacgcaattt ttctggccat cagatagatg
    41941 gccggttcct gcacaatttc atccaacaga tgaatgaatt gaatgctacg cgcctgccac
    42001 acagacgtcg cgtcatccgc cagtggcgcg aatgcccggg tcaacagggc ggtgtggctg
    42061 gcgtcaataa caatctcacc aaaagtcatc acccccagca tcttacgtcg ggcttgctcc
    42121 tcttcgacct gggcaagcca atcttcgtat tccgtcagtg ggcaggggaa ggcgacattc
    42181 agacaatcaa tcacgccaac gtgatgaccg atagccagcg agtaatacat tacctgagag
    42241 ctgtgttccg gcatctcgtc gctactggcg acaaactttt ttcgcagcgt ccagaaaaca
    42301 atttccccgc actcttcagt catgtaaccc ccggagtacg cgaaagagtt gctgaacgat
    42361 actattgaga cgtggatcgt cggcatcgtg caaccaggtg ttaacccgat ttcctgtagg
    42421 atcgtcgctt aaatggcgca ggagccgatc gcaaatttct cgcccctgac gatagcccgc
    42481 cagccgccgc gcttctcgct caatggcgat acgctgtgat ggcgggatct gtggccacaa
    42541 cggttgcata gtcaccccgg tgggatcgcg ataatccaca gcgtgaatct tctgttgcag
    42601 caaaccgagc gccacggcga aaccgtgaat ggtggcggcc ggtgttggcg ggcagccggg
    42661 gatccaaaca tcaatgggga caatggtgtc gctgccgccc cagacgctgt agagatcgtg
    42721 gaaaataccg ccgccgacac cgcacgcgcc gtaggaaaca cagattttat gatcgggggc
    42781 agactcatac gcccgaagtg caggcatacg catcgcccgg gtgactgcgc cagtaaataa
    42841 caaaatatcg gcgtgacgcg gtgatgaaac aaccttaatg ccaaaacgtt ctgcgtcgaa
    42901 tactggtgtg atggcagcaa aaatttcgat ttcacaggcg ttgcagccgc cgcaatcgac
    42961 gcgataaacg taagccgagc gacggatatc ctgtagcaaa tgctgcttca tcttttgcgt
    43021 ttgctcgtcc agcgtgatgg gctggctgac atattgcgta agcactggac tcatagctgc
    43081 tccttagcca ccagcggtac atctgtatcg tcgttgatca gcgtcgcgcg ttgtttgcac
    43141 tccgggcaga cgctcgcttg cgcccgcaac atttcgcggt tttgtggcgc attttgttgc
    43201 tgtgccaaca attcagcagc cagtgcgacg gttttttgcg gggcaaacgg gcgttcgcaa
    43261 cggctgcaac gttgtagatg gaacgtcgcg cgggtataga gatcggcttt attggtgacg
    43321 gtcagttcaa agttattggt aagctggatg gctctggtcg ggcacacttc ttcacaacgt
    43381 ccgcagtaaa tacaacgcct cagatagagc tgccaggtgc gcgaattttg ctggtcgtcg
    43441 gtctggatag tcagcgcatt tgccggacaa gcacaggcgc cgcaggcaat acattggctg
    43501 ggcatcaggt ccggtttccc gcgaaagcct gggctgacct ccagtggcgc gaagggatat
    43561 ttcaccgtcg cggttccggc gcgcataata gttttcagta acttcagcat cttctgtcct
    43621 tacttcagcg gcgaacggtt acgatcaatg ccgtagcgtt cgatctcttt atacggcacg
    43681 gtttttgact ggcgcttgcg cacatctacc agcgtcacac ggtcggtaca ggagtagcag
    43741 ggatcaaggc taccgataat cagcggtgcg tcagaaacgg tattgccgcg cagcatgtaa
    43801 cgcaacaccg gccagttggc gtaggtggcg gcacgacagc gccagcggaa caatttttgg
    43861 ttatcaccga gcatgctcca gtgcacgtct tcaccgcgtg gcgcttcaac aaagcccagc
    43921 gcgaatgcgt gaggtttata gctaaagcct tcggtcagca gcggggtatc cggcatgttg
    43981 tcgagggcaa attccagcat tgccagcgaa tcaaacgtct ctttgacacg gaccatcacg
    44041 cgggagaaaa catcgccgcc ggtgaaggta aacagtgttt ttggaatatt gccgtagtcg
    44101 gcgtaggggt gatcaaagcg caaatcacgg gcaaaaccac tgccgcggat cagcggccct
    44161 acagggctat aatcacgggc gatttgtcgg tcgagaatgc caatgccctg agtgcgttgt
    44221 tccatattcg gcatagcaag cagcatctct accagctccg acacgtcggc gcgcatctcg
    44281 cgcaccagtt tcagcgtttg cagacgttgc tctttgagaa tatcgcggcg aacaccacca
    44341 atcagattca gaccgtaggt tttacgcgac ccggtcagca attccgccat cgtcatcgac
    44401 ttttcccgca cgcggaaaaa ttgcataaag ccggtatcaa aaccaacaaa atggcaggag
    44461 aggccaaggt tgagcaaatg actgtgtagc cgttcgactt ccagcagaat cgagcgaatg
    44521 gtatgcgctc gttgcggcac ctcaatcccc agtgcatttt caaccgagtt ggtataggcc
    44581 acactgtggg caaaaccgca aatcccacac acgcggtccg ataagaaggt cacttcgttg
    44641 tagcccatcc gcgtttctgc cagtttctcc atgccgcgat ggacataaaa caggcggtaa
    44701 tcagcatcga caatttgctc gccatccaca aacaagcgga agtgacccgg ttcatcggag
    44761 gtgatatgca gcgggccgac agggatcacc cgcgcatcgc tgttgccctc attgataaac
    44821 gcatacgttt cggaatctgt cgtcggttca gggcgcagtc gataatccat cgcatctttg
    44881 cgcagcggat gcatatcttc cggccagtca tcgggcaaca ccaggcgacg ctgatccggc
    44941 aggccaaccg gaatcagccc gtacatatcg cgaatttctc gctcgcccca gaccgcggca
    45001 gggacgcgcg gtgtgactga cggaaactcc cgactgtcgg catcgaccag tgccttcacc
    45061 acgatccagc atttttcggc cccttccatt gaaagggcat aataaaccgc gtaatgaccg
    45121 ttaagtgtcc gctcgtcgtt gccaaacagg actggaagcc agccatcatg ttgataataa
    45181 agataatgta caacgtcagg cagcagattg attttcaccg taatggtgac ctgttcaggc
    45241 gtttgtcgct cttcatccag caccgcgccg gggaactgcg ttttcagggc ggcgagaatc
    45301 gcttcgccac gatttgacga tgaattaacg ttcactgtgt ttctccgaaa cagatgcggt
    45361 gcctgaaggc aaaaaatcat gccaggtgct acgttgtgca ggcaggtcgt gcgtccctga
    45421 gaggactata gtggaagcgc ccgccaggat cctgatgaca ggttgtggaa tatgcgttcc
    45481 catcgccagc atcatgacca gcagaatcac cattggcgag gtggtcaacc agccgagatc
    45541 accccggtta acggcctgcg gcggtttcgc cattaacacc cgcgcagcca tccgtaccag
    45601 gcccgccagc accagcgtta acagcaataa cagcaggacg ataatcagca ggtgattacg
    45661 tgccagtcct gcggtaacgg tcataaattc gctaagaaaa atgttgaagg gcggcatccc
    45721 tgccagcgcc agcgcaccgc cgccaaacag cacggcggta aatggcatga ttttgagcat
    45781 cccacagacg acgttgagat cgcgcgtgcc gtacttgagc agtacattgc cggaaccgca
    45841 gaacagcagc gtttttgcca gactgtggtt taaggtgtgc agcagcgcgg caaaaattcc
    45901 cagcggcccg ccaatgccta gcgccaccgc gaccagcccc atgttctcca cgctggagta
    45961 cgccagcaga cgcttaatgt cccgctgtac cagaatgaaa aatgccgcca cggcaaccga
    46021 caacatgccg aagatgagca acaaccggtt ggggaaatcg ctgccgatgg cctggcagat
    46081 aatgatgtaa tagcgaatta gcaccaacag cgcgcagttc agcaatacgg cggagagcag
    46141 ggcgctgacc ggactcggtg cttcactgtg agcatccggc agccaggcgt gcatgggaaa
    46201 tagcccggtt ttggtgccaa agccaattag cacaaacaca aaggccaaca gcattaatgt
    46261 tgggtcaagc aaggacgatt gcttaagaac ctcgctccag aatatcgcca tttctgcctg
    46321 cggcataacg ctggcgacgt tggcgtatac cagcacggta ccgaacagac caaaagcgac
    46381 accaacagta caaataatga tgtacttcca tgctgcttcc agcgatgaac gctgaccgta
    46441 aatgcctacc agaaacgccg agcttaaggt ggtggcttcg atcgccgccc acatcacaat
    46501 caggttattg ctggtaacaa ccagcagcat ggtaaacaaa aacagatgga agaaaccgta
    46561 gtaatcgcac agcgtaacgg gggaaagctc gccgtgtgcc acttcatgac gcatgtagcc
    46621 aatcgagtaa acaccggtga gaaagccaat cacaccaaga atggcgagga acaaaccgcc
    46681 cagaccatca atatgaagcc acagtcccgc agcgaatatt tctcctgcat cagcggcagt
    46741 ttggaccacc cagagtgcca gaatcagcag cagtgtgatc cctaagctat gtaatacagt
    46801 caccgtacaa gtcgcagaga gcccccgttt ccggcaggca aaacagagca gcgaaaaaag
    46861 cagcggcgtg agcaggagta aagcgaacat cacagaataa ctcatctcat tatcccttca
    46921 gcgcggtcaa gttgttcacg tccagcgtgc cgtgggtacg ccatattttt cttgccagta
    46981 acaccatcac aatgacggcg aagatggcgt cggtagcgat acctatttcc accagttccg
    47041 gtgctcgcca ggcaagaagc gccagcacca gatgggagcc gttttccatc aggcagtaac
    47101 caaaaatttg ccgcaggata ttgcgctggc tgacaatgca cagcaggcca agcagaaaat
    47161 gacctaacgc taccgccagc gccggtttca gcccggtagc catcggtagc ttcacgggct
    47221 gaacgacaaa tgcgcaaagc aggacaatta acgccgcgag cagtgccatc attgccggac
    47281 cgaataacgc tttttccggg atgttctggg gaatatttcg tgcagcgtaa gtcatgatta
    47341 acggtaccag cagcactttg gtgataaagg cgctggcgga ccagatcagc agttgctctg
    47401 cggcgaacag gcacgagaga gtggcgaaaa tagacaccag caccagcgac tggcaggcgt
    47461 aaaatccgca tgacaggcga tagcttttga cgctaatcac aaacagcgat gtcagcatca
    47521 tcagtcccgc cagattattt acgatcatag aaccggtcat tcatcatctc cttactgcaa
    47581 ccaggtcgcg gcgattacgc tggaaatcag cgacatcaca atcagtacaa tcaacaccag
    47641 gcgcattgac cctggcagcg gtgcggcatc ggcgacggcc tcgctcggtt tgccaggcac
    47701 aacgcgacca aaccagcgaa taaaccaggc gaaactggcg accgattcaa tcatcagcag
    47761 aatcatggcg ggcagcagga tccagtactc cactgacaac gcaaaaccgg cagcaaacag
    47821 cgggaattta ctgaagaagc cgttgaacgg cggcacgccg gtaatcgcca gcgctgccac
    47881 gcagaaaccc acgcctggca acggcagggt gtgcaatacg ccacgcagac gcggcaacaa
    47941 gcgcgtgccg cagctgtaac tcagcgcacc tgctacaagg aaaaacaggc ttttagcgaa
    48001 cgcgtggttg acgatgtagg cgatgctacc ctccagcgcc agccgcgagc cgaagatgga
    48061 gagcgacaag ccgaagaaca tccagccaag ttgagtgatg gtcgaccagg ccagcaaccg
    48121 cttcatatcc tgctgtggca aatacatcag aaagccataa agaatggtga ccagtgccat
    48181 gaccatgcca acgccgccaa tcacatgcgg gatattgccg ccgtcgataa ttgcgcgggc
    48241 aaaaatgtaa acgcccactt tcaccatcga tgcggcgtgg agataggcgc tgatcggtgt
    48301 tggcgcttcc attgcatccg gtagccacgc ttgcatcggt agctgggccg atttccccca
    48361 cgcggcaaac aggatcccgc cataaaccag ataacgtgcg tcgccgtgta actcgctcat
    48421 tgcgctaagc gcaaacgttc cggtctgcaa aaacagcgtg gcggcggcaa gatacaaccc
    48481 caacgaaccg atatgggtga taagcaacgc ttttagtgct gaacgctgcg ctttatcgct
    48541 ctggtaataa ctgatcaacg cccaggagca gccgccctaa tttcaaaaaa caacaactga
    48601 ccgagcagcg tcgaggagag taccagtccc gccatcgcgc cgataaacac cagcaaaaat
    48661 gcgtaataac gattcgtgcc gttatgcggg tgttcgcgat ttttatccgt cagataaccc
    48721 gtggagtaga tcgtgaccag caaaccgaga aacaccacca caaacagaat cagcgtactc
    48781 acgcgatcaa tgaccaggcc aaacaatgcc acatccccca cgttgaccaa cgtcaacgtg
    48841 acggcaactt tatcggcctg ataaaacgcg gagatcagcg acaacatgca cagcgtggtc
    48901 agtgcggcga acaaaacccc cattcggcgg cccgacgttg tggcgaaaac gaaacgacca
    48961 gtgcgccgat aaaaggcagc attaacgtcg tcagagcaag attttccata ttccgtcagt
    49021 gctccttaca gaccggttaa ccagaagacc catgcaagcg cagcaaggct gaagccaagc
    49081 caggtcacat ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc tgcgattgag
    49141 gccagtacaa aaatcagcaa aaccttgagc agcgtaacga caagtgaagt cagcaggcag
    49201 gcgagagaaa gttcttgcgc gcgcccaaag ggcagaaaca gggccacaaa cagtgctgcc
    49261 atcacgacct gtttcagccc cagcccccat ttcgctagcg ccagcccggc accggaatat
    49321 tcggtcagcg ggccttcctg taattcctgt tctgcttcag caacatcaaa gggaattttt
    49381 cccatctcaa tgaagcaggc aaaaccacag gccagtaacg ccagtacggt ggttagcggc
    49441 gagttccagc ccgttgccag cgtattgctg atcatctcga tatgcgtgga acctgctatc
    49501 agcgccaata ccagcagtga gagaatgagc atgggttcga ccagaatgcc gagcgtcaac
    49561 tcgcgactgg caccgactcc cgcaaacggg cttccggtat ccagcccgga aagagcaaag
    49621 aaaaaacgaa acagggcaag aagatagata agggtgatca gatcgccgcc gcccgcaaaa
    49681 ggggaaacgg taataaaaag tggtagggcc atcgccagca ccagcatgct gctgattaat
    49741 acccacggca tcaggcggaa catcagacct gaagatatag gcgcaacttc ctggcgttta
    49801 aacagtttgt ggatatcgcg ataatcctgc cagatccccg gtccgcggcg ggagtgcatt
    49861 cgcgcgcgta tctgccggga aatacccgta aaaagtgggg ttagcatcag cagaatcacg
    49921 gcctgtgcta acgcaaaaat gaccagatat ccgtcgcaaa gagtttgtct catggtgatt
    49981 tccttagacg gcaatagcga ttagcagcac aaccagtgcg gcaaccacat acaggcaata
    50041 gagacgaaag tcgccgcttt gcagatgctg tatttctttg gccagccgtt gggtggcgct
    50101 cacgatgggg cggatcaccc gctcatccca gaagggttct gtgctctgag ccctggcggt
    50161 gacgtgcgca agacctttat ttagcctcag cgtagggtcg agctgttgtc gtagacgaaa
    50221 tagcgcagaa aagaccacac gcagcggctg catcacgcca ttgcctgacg gggccatcgc
    50281 attttcccag ccgtagccgc atgcccaggc atctcctgtg cgacgaaacg ctgcgcggcg
    50341 cgaacgacac cacagccagt aaaggccagg cagcaaaggc atcgccagta gtaacagcaa
    50401 cagtaatgag ggggtgaccc gtgtatgaaa cgtgccgggt acaagtgcta ttccgctggc
    50461 gacagtgacg ggaggggtat tggtaaacgc atgggcaata tgcattatct tcggtgccag
    50521 ccaactggca ctaagcgcaa ttaatacgca gagtgccgcg agcagtagca tggcgacgat
    50581 catcgtattt ggcacttcct gtgcctcttc agcgtgtgta ctgcgcggcg caccacagaa
    50641 agtaataccg tacattttga cgaagcacat tactgccagc ccaccagtga ctgccagcat
    50701 taccatagca ataggacccg caagttgtag cgctacggct tccacacgac ttagtgagaa
    50761 cagcgactgc caggtgtacc attcgctgat aaaaccattc agcggaggaa tggctgatat
    50821 cgcgaggcaa ccaatcaggc atgctgcggc tgtccacggc atccgtttcg ccagtgcccc
    50881 cattttttcc atgtcgtggg tatgcaaacg cgaaataatc gcacccgcgc cgagaaatag
    50941 cagccctttg aacagcgcat ggttgagcag atgaaacagt gcgccgagca gtccaaccac
    51001 ggtgagcagc gggtcgtgca gtgacagacc gtccatcgcc acaccgaccg ccagcaaaat
    51061 aatgccgacg ttttcgacgg tactccaggc cagcagccgt ttgatatcct gttccgccag
    51121 tgcatacagc acgcccagga gcgcggagat tgcgccgacc gccatcacca gagtgcccca
    51181 ccacagaggc aaacccgttt gcgccagcag atccatcgct actttcagga tgccgaaaat
    51241 accgattttg accattacgc cagacatcaa cgccgaagcg tgcgatggtg cggcagggtg
    51301 agcgcgcggc aaccagctgt gcaacggcat catcccggct ttcgcgccaa aaccgaaaaa
    51361 ggccagcaga aacaccgccg acgccagccc cggagaaagt gaaagcgtgc gaaactggca
    51421 aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcataat cagcaccgat
    51481 ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt atcctgcctg
    51541 gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat gaaccaaaaa
    51601 gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaagattcat aaagaagccc
    51661 atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata gagcgaacaa
    51721 accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc cattcgcagg
    51781 gttaaatcgg caaagtcaaa aggggtggca aaatgggcga ctaaagttac accagcatgt
    51841 aattgcgtaa ttccgctgat gatgccaatc acgccgccca ccagcgatgt gatgccggaa
    51901 agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact ggcaaacaga
    51961 tagaggatca gcgaccaggt taataattgc aaggcatcca ttagtgttgc tcctgagtga
    52021 gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc tgtttttctt
    52081 tcatctgacg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc gggcaagcgc
    52141 gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc gcgacggtct
    52201 ggacacccgg ttcccggcgc agcaacgcat gttggggcag cacgttttct tcgccgtctt
    52261 tgagtgagcc ttcagcctga aaaacatatt gcgcatgggc attcaccgga cggcttcctg
    52321 aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca atacagaggc
    52381 tttcgttgag ttggatcgca tcatccctct gggagatggc attaaccggg cagacctgca
    52441 ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt gacgtcttcg
    52501 ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc gaacaggcag
    52561 cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag cggttcatgt
    52621 cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga tgccattctg
    52681 attaactcaa tatattcaat aagttatgta ttgactggat tctgaacggt acaaaaatga
    52741 tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg aaagcgagaa
    52801 tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag cacggaatgg
    52861 agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg tgattgctgg
    52921 ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg aagctgatgc
    52981 gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg ccgaattgtt
    53041 cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct ttttccgcaa
    53101 aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg cccgcttttt
    53161 tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg ggggtcatgg
    53221 ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa ttaacttgtt
    53281 ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc agtggattca
    53341 ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa tactgccttg
    53401 tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg cgtccgcaga
    53461 tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat tttcagcagg
    53521 ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa gtgctgtgaa
    53581 gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag atgctggcat
    53641 tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca gttcggcacc
    53701 tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg aaagcagcat
    53761 aataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat tacagccgca
    53821 actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac ccaacgcagt
    53881 gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa ggggtgagtt
    53941 gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt ctgtctgctt
    54001 gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag gatggcccat
    54061 gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta atgtctgtcg
    54121 ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg cgatcgtttc
    54181 tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg atgtggtgat
    54241 gatgacgctg gagctggctg atgggcgcat tccggtaatt gccgggaccg gcgctaacgc
    54301 tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg tcggctgcct
    54361 gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc atttcaaagc
    54421 catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc gtactggctg
    54481 cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta tcggaatcaa
    54541 agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt cagatgattt
    54601 tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattgg gcggtcatgg
    54661 ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt gcaaactggc
    54721 agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc cattacacaa
    54781 caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg aactgggtct
    54841 tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg gtcgtgagac
    54901 ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat ttgatggctt
    54961 actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt ttattactcg
    55021 ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa gcctacctgg
    55081 aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg gtgacctccg
    55141 gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg ctggacattc
    55201 gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc acgggcgata
    55261 ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag gtggttagcg
    55321 tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag acactgacca
    55381 ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt cgttatcaaa
    55441 tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgctg aacctggaac
    55501 aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg gagatgatga
    55561 acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct gcgcaaaatc
    55621 gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta ccgatgctgg
    55681 tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg gaaaaagtgg
    55741 gcatgaaagt gaccgacagc acccgttcgc agggtaacat ggccgtaact tataagccgc
    55801 tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca tccggtgact
    55861 ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc gatccgaaag
    55921 gtcatactct gactcagagt cagaacgacg cgctggtatc tgtcttccag gctgcgttta
    55981 gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga tacgcaaacg
    56041 tgtgcgtagg caggaaaacg cgattttagc ggtaattcgc acgaaatttg tttgtcggac
    56101 gtagttcgaa taaggcgttc acgccgcatc cgacaaaaca tccggcacac cagacagcaa
    56161 aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc aagctgagtt
    56221 gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt tggtgctcga
    56281 attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg atcgcaaagg
    56341 tatggtgaac aacaagttca actacttcat tatgagcaaa ctggctgaag cgggtatccc
    56401 gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc tggatatggt
    56461 gccggttgag tgtgtcgtgc gtaaccgtgc tgctggctct ctggtgaaac gtcttggaat
    56521 cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa acgacgccat
    56581 gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga gcaaagagaa
    56641 cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa aactgttcga
    56701 tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggcctgtaca aaggtgaagt
    56761 ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag aaacactgga
    56821 gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag cctatgaagc
    56881 cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tggttgcttg tttctttagc
    56941 cggagggtac tccggctaaa tgtctttgat acccccgcta caatacttct cattatctcc
    57001 ctttgacagg aaagtcatac caggccaata tgtatataca taacgtatat cccaaaaagg
    57061 gggggctatg gaaactactg tatttttaag taatcgcagt caggccgtca gattaccgaa
    57121 agcggtcgct ctgccagaag acgttaagaa agtagaaatt attgcgattg gtcgtacaag
    57181 aatcatcact cccgccggtg agtcctggga cagttggttt gatggcgaaa atgtgagtgc
    57241 ggattttatg gatatccgtg atcaacccgc aatgcaggaa agggaatcat tttgatgctg
    57301 aaattcatgc tggataccaa tatctgtatt ttcactatta agaataaacc agtacatgtc
    57361 agagagcggt ttaacctgaa ttctggccgg atgtgtataa gttctgtaac attgatggag
    57421 cttatctatg gggcagaaaa aagtcagatg ccagaacgca atttggcggt tattgaaggt
    57481 ttcgtatctc gtttggtagt gcttgattat gatagtgctg ctgcgacgca taccgggcaa
    57541 atcagggccg agcttgcccg gcagggaagg cctgttggac cttttgacca gatgatagcg
    57601 ggacatgctc gcagtcgcgg tttgattgtg gttaccaaca atacccgaga gtttgagcga
    57661 gtggcgggaa tcagactaga agactggagc taacattctc ttttacttct aacattcgta
    57721 aattatataa cgtggcatga agccttaatt cctccgccat tttatcccgc tttcctgtgg
    57781 taatcttcct ctgaaccacc tacgatcatt tctataatga atatattgtt gaggtatcta
    57841 tgcgttggca agggcgacgt gaaagtgaca atgttgaaga caggcgcaac agctctggcg
    57901 gtccgtcaat gggcggtccc ggtttccgtc tgccgagcgg taaaggcggg ctgattttac
    57961 tgatagtcgt gctggttgca ggctactatg gcgttgattt aaccgggttg atgaccgggc
    58021 agccggtttc tcaacaacaa tcaacgcggt caattagccc taatgaagac gaagccgcaa
    58081 aattcacctc ggtgattctg gcaaccacgg aagacacctg gggacaacag ttcgagaaga
    58141 tgggtaagac ctatcagcaa ccgaagctgg tcatgtaccg tggaatgacg cgcaccggct
    58201 gcggggctgg tcagtccatt atggggccgt tctattgccc ggcggatggc acagtttata
    58261 tcgatctctc cttctatgat gacatgaaag acaaacttgg cgcggatggc gattttgccc
    58321 aggggtacgt tatcgcccat gaagtcggtc atcatgtgca gaaactgtta ggcatcgagc
    58381 cgaaagttcg tcaactgcaa caaaacgcga cgcaggcgga agtaaaccgc ttatctgtgc
    58441 gtatggaact ccaggccgac tgttttgccg gtgtctgggg gcatagtatg cagcagcaag
    58501 gcgttctgga aactggcgat ctggaagagg cgctgaatgc ggcgcaggcc atcggcgatg
    58561 accgtttaca acagcaaagt caggggcgag tagtaccaga cagtttcaca catggcactt
    58621 ctcagcaacg ctacagctgg tttaaacgtg gtttcgacag cggcgatccg gcacaatgca
    58681 atacttttgg taaaagcatt taatttccgg ggcagggatg gctgaactga ctgcgcttta
    58741 cacattaaca gcgcaaatga aacgtgaagg gatccgccgc ttgctggtgt tgagcgggga
    58801 agagggttgg tgttttgatc atgcgcttaa gttgcgtgat gccttacctg gcgactggct
    58861 gtggatttcg ccgcagccag atgctgaaaa ccactgttca ccctcggcgc tacaaacttt
    58921 acttgggcgc gagttccggc atgcggtatt cgacgcccgc cacggctttg atgccgctgc
    58981 ctttgcagca cttagcggaa cgttgaaagc gggaagctgg ctggttttgt tactccctgt
    59041 atgggaagag tgggaaaacc aacctgatgc cgactcgttg cgctggagtg attgccctga
    59101 ccctattgcg acgccgcatt ttgtccagca tctcaaacgc gtacttacgg cggataacga
    59161 cgctatcctc tggcggcaaa accagccgtt ctcgttggcg cattttactc cccgtactga
    59221 ctggcacccc gcgaccggcg caccacaacc agaacaacag caactcttac agcagctact
    59281 gaccatgccg ccgggcgtgg cagcggtaac ggctgcgcgt gggcgcggta agtcggcgct
    59341 ggcagggcaa ctcatttctc gtattgcggg cagtgcgatt gtcaccgcgc ccgcaaaagc
    59401 ggcaacggat gtactggcac aatttgcggg cgagaagttt cgctttattg cgccggatgc
    59461 cttgttagcc agcgatgagc aagccgactg gctggtggtc gacgaagccg cagccatacc
    59521 tgcgccgttg ttgcatcaac tggtatcgcg ttttcctcga acgttgttaa ccactacggt
    59581 gcagggctac gaaggcaccg gacgtggttt tttgctgaaa ttttgcgctc gctttccgca
    59641 tttacaccgt tttgaactgc aacagccgat ccgctgggca cagggatgcc cgctggaaaa
    59701 aatggttagt gaggcactgg tttttgacga tgaaaacttc acccatacac cacaaggcaa
    59761 tatcgtcatt tccgcatttg aacaaacgtt atggcgaagc gagccagaaa cgccgttaaa
    59821 ggtttatcag ttattgtctg gtgcgcacta ccggacttcg ccgctggatt tacgccggat
    59881 gatggatgca ccagggcaat attttttaca ggcggctggc gaaaacgaga ttgccggagc
    59941 gctgtggctg gtggatgagg ggggattatc tcaacaactc agtcaggcgg tatgggcagg
    60001 ttttcgtcgc ccgcggggta atctggtggc ccagtcgctg gcggcgcacg gtagcaatcc
    60061 actggcagcg acattgcgtg gacggcgggt cagccggata gcagttcatc cggcgcgtca
    60121 gcgggaaggc acagggcagc aacttattgc tggtgctttg caatatacgc gtgacctcga
    60181 ctatctttcg gtgagttttg gttacaccgg ggagttatgg cgtttctggc accgctgcgg
    60241 ttttgtgctg gtgcggatgg gtaatcatcg tgaagccagc agcggttgct atacggcgat
    60301 ggcgctgtta ccgatgagtg atgcgggtaa acagctggct gaacgtgagc attaccgttt
    60361 acgtcgcgat gcgcaagctc tcgcgcagtg gaatggcgaa acacttcctg ttgatccact
    60421 aaacgatatc gtgctttctg acgacgactg gcttgaactg gccggttttg cttttgctca
    60481 tcgtccgcta ttaacgtcgt taggttgctt attgcgtctg ctacaaacca gtgaactggc
    60541 attaccggcg ctgcgtgggc gtttacagaa aaatgccagt gatgcgcagt tatgtaccac
    60601 acttaaactt gcaggccgca agatgttact ggtccgtcag cgggaagagg ccgcgcatgc
    60661 gctgttcgca cttaatgatg ttcgcactga gcgtctgcgc gatcgcataa cgcaatggca
    60721 attttttcac tgactccttc aattttctcc catggtcatt gtgagccagc ggcgtaaaat
    60781 taatgacatc agttaaggag ttcgccatga aacatgacca ttttgttgtt caaagcccgg
    60841 ataaacctgc gcaacagttg ttgctgcttt tccacggtgt cggggataac ccggtggcaa
    60901 tgggggaaat aggtagctgg tttgcaccgc tgtttcctga tgcgctggtg gtgagtgtcg
    60961 gtggcgcgga gccgagcggt aatccggcgg ggcgtcagtg gttttcggtg cagggtatta
    61021 cggaagataa tcgccaggcg cgtgtggatg cgatcatgcc gacgtttatt gaaacggtgc
    61081 gctactggca gaaacagagc ggagtagggg ctaacgccac ggcgctcatc ggtttctctc
    61141 aaggcgcgat tatggcgctg gagagcatta aagccgaacc gggccttgct tcacgcgtca
    61201 ttgcttttaa cggacgctat gcgagcctgc cggaaactgc gtctacagcc acgacaattc
    61261 acctgattca tggcggtgaa gatccggtta tcgatctggc gcatgcggtt gcggcgcagg
    61321 aagcgttaat cagtgccggt ggtgacgtga cgctggatat cgtggaggat ttaggccatg
    61381 cgatcgacaa tcgcagtatg caatttgccc tggatcactt gcgttatacc attccgaaac
    61441 attatttcga cgaagcgcta agtggcggta agcctggcga tgatgatgtg attgaaatga
    61501 tgtaaaagac agattacctg gcctgataag acgcgtgagc gtcgcatcag gcatcgttca
    61561 ccattgccgg atgcggcgtt aacgccttat ccggcctacg gttgagcact cagcttactt
    61621 cttcggttga tcctttttcg gccagtcgtc gtcatcgtcc cacttatcgt tgaaatcacg
    61681 atgcggggga agctccggct tgttggcgag aaatttctta tggtcgacgc gcttgagatc
    61741 tttaatcacg ttcagcagga cgcctaccaa aaagacaatc accagaatcc accaatattt
    61801 agccagccag tccatgctta tttcctcttg cagaaccact catcaggcga cgagctgttc
    61861 catgatacgt tgatacatac gggcaagtag ctgcaggtcg gcagcgttca cacattcatt
    61921 aattttatga atagtggcat tgaccggccc gagttccacc acctgcgccc ccatgcgagc
    61981 aataaagcgc ccgtcggacg ttccgcctgt ggtcagtagc tgcggtttaa tttcattata
    62041 gtgctcaacc gcgttaacga ccgcatccac cagtttaccg cgcgcggtca aaaatggctg
    62101 cccggaaagc caccaatcca ccgtatagcg cagttgatgt ttttcaagca gggcaagcac
    62161 ctgcgctttg atcatctcat cagtcagttc ggtgctgaag cggaagttaa actgcacaaa
    62221 cagttcaccc ggaataacat tgttactgcc cgttcccgcc tgaatattag caatctgcat
    62281 actggtcgcc gggaaaaatt cattgccctg atcccactca atagccacta attcattaag
    62341 gaaaggtgct gcgcgatgta ctggattgtc agccagatgc gggtaggcca catgcccctg
    62401 aacgccatga atggtaaggt tgcaggttaa cgatccgcga cgaccatttt tcaccacatc
    62461 accaaccact tcgatactcg acggttcgcc gaccaggcag taatcgagac gctcattacg
    62521 tgccatcaac gcttcgacga cttttaccgt accgttgtgg gcactggctt cttcatcaga
    62581 ggtgatcaga aatgccagtc gccccgtatg gttgggatgt tgtgcgacaa agcgttctgc
    62641 cgctaccacc atcgccgcca gcgagccttt catatctgcc gcaccgcgcc cgaataacat
    62701 gccatcacga atggtgggtt caaacggcgg attgatccaa cgatcggcgt cgccaggcgg
    62761 caccacgtcg gtatgcccgg caaaggctaa cgtttcacct tgcccacgcc atgcccaaaa
    62821 attctgcgta tcggcaaagt ccatgcgttc aacggtaaaa ccgatcgcct gcaaacgttc
    62881 aatcaacaaa gcctggcatc ctgcgtcatc aggactcagg gaagggcggc gaataagctg
    62941 ttgtgtcagc tcaataaccg ggcacgacat agactacacc tcatggaaaa attgctgata
    63001 actggaatca ctgaaaccca gcagcatagg cttaccaggc gcgcagagca atggacgttt
    63061 gataattgca ggcatttcag tcattaatgc cgccgcagag gccgcatcgg tgattttatt
    63121 gcgggtggtt tcgtccagtt tacgccaggt tgtaccacgg gtgttcagta acgcttccca
    63181 gcctaattcg ttgataaaac cgttcaataa ttcgctgtcc agcccatcga cgcggtaatc
    63241 atgaaaacga tagtcgatgt tattggcttc cagccaacgg cgagcctttt taatggtgtc
    63301 acaatttttg atgccgtaaa gtgtaaccat aatgaatcct ttttcgatct atgagaataa
    63361 atactcattt aagggtgaaa acattttgcc tgcaaatgat tatcaggaaa tataacatta
    63421 tcgaagtatt caatttaaca actattaaat aatattaaac atttttaacg taaacattaa
    63481 gaatattcat cttgttatct gtctgtattt taaccagaat taaatcagta ttagatgccg
    63541 tatgaatatc tcgaaatgtt gctgtgtatc acatacaaat tatcaccgca ggaccatagt
    63601 atttacataa ggtttgtccc ccggaggtag tcatcgcagc aatcgagtaa attagcttca
    63661 ccacggtata ttgtttccgt agaataccca taattataag agaggttgtt atgattgaac
    63721 gtgaactggg gaactggaaa gactttatcg aagttatgct tcgtaagtaa ttcccgagac
    63781 aaagtataaa cagcaagaac ccgctgagca ggttcttaat cggaaagttg aaaaaggcga
    63841 cacattggca tgtcgccttt tttattgctt attccgggcg cggcttcagc gggaagcggc
    63901 ggcgcaccag cacaaagaac agcggcacga agtaaatagc cagaatagta gctgaaatca
    63961 tcccgcccat tacgccagta cccaccgcat gctgaccacc ggaaccggca cccgtgctgg
    64021 ttgccattgg caatacgccg aagataaatg ccagcgaggt catcagaatc gggcgtaaac
    64081 gctgacggca ggcgtggagc gtcgcttcaa acaggtcgtg gcctttttgg ttcatctcgt
    64141 tagcaaactc gacgatcagg atggcgtttt tcgccgataa accaatgacc gttaacaggc
    64201 ccacctggaa gtaaacgtcg ttttccagcc cgcgcatcca ggttgccagc agcgcgccga
    64261 ttacccccag cggcacgacc agcattaccg agaacggtac cgaccagctt tcatacaatg
    64321 ccgccagaca caggaatact accagcaagg aaatggcgta cagcgccgga gcctgcgcgc
    64381 cggaaagccg ctcctgatac gacatcgccg tccactccag accaaagcca tttggcaact
    64441 gcttcactaa cgattccata atatccatcg ccgtaccggt actgaccccc ggtgcggctt
    64501 ctccaacaat ctcaaccgca gaataaccgt tatagcgttc cagacgcggt gagcctgtct
    64561 cccagcgtga ggttgcgaaa gcagagaagg gcaccatgcc gccatcttta tttcggacat
    64621 accagagatt aatgtcatct ggcagcatgc gatacggcgc agctgcctgc acatagactt
    64681 tcttcacgcg accgcgatcc ataaagtcat tcacatagct cgaaccccag gcggtttgca
    64741 gtgtgtcgtt aatatcgtcg atagcaacgc ccagcgcctg agctttacgc tggtcgatat
    64801 caatctgcaa ctgcggactg tcatcgaggc cgttatggcg cacacgggtt agcttcgggt
    64861 tttccgccgc cagcgctagc aactggttac gtgctgccat cagcgcatcg tgacctgctc
    64921 cagcgtggtc ctgcaactcc atatcaaaac ccgcagaact accaagaccg ctaattgctg
    64981 gcgggctgct ggcgataacg cgagcttctt taatttggtt aaacgccttc gttgcacgct
    65041 cgataatggc aaacgaggtg ccggtcttac tgtcgcgttc gctccagtct ttcaggcgga
    65101 taaacattct cgccacgttt tgcccgttgc ccccagggcc agaaccaacg gtggcaaaca
    65161 ccgacatgat gttgtctttt tcatgggtga agtaatattt ctcgatttgc tcaacgactt
    65221 tcagggtctg ttgttgggtt gaaccgctgg gcaattgtac cgaggtagta aacatgccac
    65281 ggtcttccag cggtaagaac gacgtcggca aacgcaggaa caggaacacc atgccgccaa
    65341 gcagcaggac ataaatcaca atccagcgca ggctacggtg gagaattttc gccacccctt
    65401 tttcgtagcg ttcggcgttg cggttaaaca tctggttaaa ccaggcaaaa aagccttttt
    65461 gcccgtgatg ctcacctttt ttcagtggct taagtagtgt ggcacacaga gccggagtga
    65521 ggatcatcgc taccagtact gacagcacca tcgccgcaac aatggtaata gagaactggc
    65581 gatagatggc accggtggtg ccgccgaaga aggccattgg tacaaatacc gccgaaagaa
    65641 ccatcgcaat cccgaccagt gccccctgga tctgccccat cgatttacgt gtggcttcgc
    65701 gaggagtgag tccttcctca ctcataatac gttcgacgtt ttccaccacc acgatggcgt
    65761 catccaccag cagaccgatc gccagcacca tcgcgaacat ggttaaggtg ttgacgctgt
    65821 aaccaaaggc gtaaagtacg gagaaggttc ccatcaacac caccggcacg gcgatagtgg
    65881 gtatcagcgt ggcgcggaag ttttgcagga acagatacat aacgaggaaa accagagcga
    65941 tagcttccag cagcgttttc accacgtctt caatggaggc tttaacaaac gaggtggttt
    66001 catacgccac cttgtattcc agtccgtgcg ggaagtactg cgccagctcg tcgagacgat
    66061 tgagcaccag ctccgctgtc gccatttcgt ttgcgccgga ggccagtttt acccccagcc
    66121 cggaggctgg cttaccattg aagcggctaa gataatcgta tttctccgcc cccatttcga
    66181 cggtggcgac atcgcccagc cttacctctg agccgtcttg attcacccgc aaggtgatat
    66241 cgcggaactg ttctggtgtt tgcagcagtg actgggcgtt aatggtggcg ttgagcgcct
    66301 gcttatcgac ggaaggtgta ccaccaagtt gcccaaccgc aatctgcgcg ttctgtgact
    66361 caatggcatc agtgacatct ttagccgtca tctggaaact gttgagtttc gccgggtcca
    66421 gccagatacg catggaatat tgcgaaccat aggcatcgat atccccgacg ccattcacgc
    66481 ggcttaacgg gtcctgaata ttactggcaa cataatcggc aatatcctgt ttatccatcg
    66541 aaccatcggt agagacgaaa gcaatggtca gaatattggt atcgccggtt ttacgcaccg
    66601 tcacgccctg attttgcacc gcctgcggta actttcgcat ggctgattgc agctggtttt
    66661 gtacttgctg cacggcttcg tccggatcgg tgcctgcttt aaaacttaaa gtgacagatg
    66721 cctgaccggt gccactgctc tgagatgaca tatacatgag attatcgagg ccggtcatat
    66781 tttgctcgat aacctgggtc acggtgtttt ccagcgtctg ggccgatgcg ccgggatagt
    66841 tagcggtcac tcgcacattc ggtggcgcga gatcggggta ttgttcaacg ggcaatgaaa
    66901 aaatcgccag ggtccctgtc agacacaaca ggattgccag cacccaggca aaaatggggc
    66961 gatcaataaa gaaattcgcc attaaaagag gacctcgtgt ttctgcgtat cgttgtaggc
    67021 ttgacttgct tcactgtagc ggcaaggtac gagccaatcg tggagaaaaa aaggagttaa
    67081 tgtaaattat catgcccttt tatactgaac cttaagtgca agtattcttt ggtcagtagg
    67141 aggcattaca tcaactgact ttcctcaccc tccgcagagc gaaagctaat actcactaac
    67201 gtgccgccgc cggaaggttg tgaaaaagtc agcgtcccac ccagccgttc cgcgcgttcg
    67261 cgcatgatat tcagaccata atgaccttcg ggttctttcg gttcaccgat accaatcccg
    67321 ttatcacgga tataaaccgt gtgattgccg tccggcgcgg tgacgcaact gacggcgatt
    67381 tcgctggcgt tggcgtgctt catcgcattc agcaccgctt cgcgaataat ttgcaacaaa
    67441 tgcacctgca tttgcgcatc cagcgccagg gttggcagac ggcagtcgag ggtcagtttg
    67501 gcgctggttt gattttgtaa cgtatccagc atttccctca acgcggaggg gagatccgcc
    67561 tgctgcagcg tcaggcggaa agtagtcaac agctcgcgta actgccgata agcatcattc
    67621 aacgcctggg aaaaatcggc catgatactt tgtgcggtgg cgttatcttc cggtatcgaa
    67681 cgcttcagta acgtcaactg gatacgtaag taagaaagta cctgagccag cgagtcgtgc
    67741 aattcgcggg cgatggttgc acgttcttcc atcaacaata actgctgaaa atgcttctgc
    67801 gcctgattaa agtacaaacc gcgtcccagc atcgatgaaa cactgttaag cagcggttct
    67861 gatgatgaaa cgtgactatt ttgccagtgc agttcgccgt aaaccgtctc ttgcattgtc
    67921 accggtaaaa tctgcatcgg caattccggg tttggttgcc cttcgctaat ccgccagttt
    67981 tcaccgacat ttaactccag atattcagcc gcttcattgt cgcgaacaat ctgcaaaata
    68041 tggcggaaac aatgcacatc aatctggctg gtgttcagcg cctgggaaca ttgatacaac
    68101 acctccagac gacgcttggc ctcgtggaga tcgcgggtct tttcttctac tgacgcctcc
    68161 agcgaaaggt acaatttatg cagctcgctc gacatctggt taaaggtttt tgcaagcaga
    68221 ccaagctcat tcggcagact ggtatccagc ggcggcgagt cgaactgccc gtgttcaata
    68281 cgttgactgg cggtaaccag ctgattcagc ggggcaacca cctgatggcg tatacggcgc
    68341 aaagtaaaaa agaccagcgt gaaaataccg ataccgccag ccagggaaat cgccaccacc
    68401 agcagcattt tgcgttcagc gtagtgctgt aaagccagca cgaacaggtc tatctgatta
    68461 acataattat taatattggc ctgataccac ggcaaatcgc ccttgctgag ccgattattc
    68521 atctccagcc agttggcatt cagatgcgca tagcgagttt ttactgcttc tggcacatac
    68581 cagacgttga gattggttaa taccggtgaa tgcagtgcct gctgaaacaa ctggcgatgt
    68641 gcattgagtt gtggactgcc actttgcaag tcgtagccca ggcggtaact ctgcatacgt
    68701 agcgatccgg caatattgat agcctcagcg tcgcgcaaac tgctcgccag agttagcaga
    68761 gcgatacccg tggaaagaat cgacagcagc acaatgtaaa aaaaggcccg ggccagactg
    68821 gccgagacgg gtcgtttaac aatcacacgc gtcttctcca caaaaaatca ttaatgttcc
    68881 agttcgctga aacacaattt agtcagtgat gggattattt gatctgccac agtttatagc
    68941 gaaatagtta cttattctgg gcacatgaac attgccgatt cccctgtaag tcgggtaata
    69001 acaacactca tataaagaat aaggttttta caaccaaaaa agaaggtcgt tatgaatcgt
    69061 tttattatgg ccaacagtca gcaatgtctg ggttgtcatg cttgtgaaat cgcctgtgtc
    69121 atggctcaca atgatgagca acatgtcctg agccaacacc attttcatcc ccgaattacg
    69181 gttatcaaac atcaacagca acgtagtgca gtgacctgtc accattgtga agatgcgccc
    69241 tgcgcccgta gctgccctaa tggcgcaatc agccacgttg atgacagcat tcaggtcaat
    69301 cagcaaaagt gtattggctg taaatcctgc gtggtggcct gtccttttgg tacgatgcaa
    69361 atcgtcctga cacccgtcgc ggcaggaaaa gtaaaagcca cggcgcataa atgcgacctt
    69421 tgtgcggggc gcgaaaacgg tcctgcctgt gttgagaatt gcccggcgga cgcgctgcaa
    69481 ctggtcactg acgtcgcact ctccggcatg gcgaaatccc gccgcttgcg caccgcgcgt
    69541 caggaacatc aaccgtggca tgccagtacc gcggcgcaag aaatgccggt aatgagtaaa
    69601 gtcgaacaaa tgcaggcaac gcccgcgcgt ggcgagccgg ataaactggc gattgaagcg
    69661 cgcaaaaccg gttttgatga aatttatctg ccatttcgcg ccgaccaggc acaacgggaa
    69721 gcctcgcgct gccttaagtg cggcgagcac agcgtttgtg aatggacctg cccgctgcat
    69781 aaccatatac cgcagtggat tgaactggtg aaagccggaa acatcgacgc cgccgtcgag
    69841 ctttctcacc agaccaacac cctgccggaa attaccggac gcgtttgtcc gcaagaccgt
    69901 ttgtgtgaag gtgcctgtac tattcgcgat gagcacggcg cggtaactat cggcaacatt
    69961 gaacgctaca tttcagatca ggcgttggcg aaaggttggc gtcctgactt aagccatgtc
    70021 accaaagtgg acaagcgggt ggcgattatc ggtgcaggtc cggcagggct ggcctgtgcg
    70081 gatgttctga cccgcaatgg cgtgggggtg acggtgtacg atcgccatcc agaaatcggt
    70141 ggcttgctca ctttcggcat tccttctttc aaactggata aatccctgct ggcacgccgt
    70201 cgggaaatct tcagcgcgat ggggattcac ttcgaactca attgtgaagt gggtaaagat
    70261 gtctctttgg attcgctttt ggaacaatac gacgcggtct tcgttggcgt aggcacttac
    70321 cgttccatga aagcgggttt acccaatgaa gatgcgccgg gcgtttatga cgcgctgccg
    70381 ttcctcattg ccaacactaa acaggtgatg gggctcgaag agctaccgga agagccgttt
    70441 atcaataccg ccggacttaa cgtcgtggta ctgggcggcg gcgacaccgc gatggactgt
    70501 gtgcgtaccg cactgcgcca cggcgcgagt aacgtcacct gcgcttatcg tcgtgatgaa
    70561 gctaacatgc caggctcgaa gaaagaagtg aagaacgccc gcgaagaggg ggccaacttc
    70621 gaatttaacg tccagccggt ggcgcttgag ctgaatgaac aaggtcacgt ctgcgggatt
    70681 cgtttcctgc gcacgcgtct tggcgagccg gatgcccagg ggcgtcggcg tccagtgccg
    70741 gtggaaggca gtgaatttgt catgccagcc gacgcggtga ttatggcgtt tggcttcaat
    70801 ccgcacggga tgccgtggct ggagtcgcac ggtgtaacgg tagacaaatg gggccgcatc
    70861 atcgcggatg tggaaagcca gtaccgttac cagaccacca atccgaaaat cttcgctggt
    70921 ggtgacgccg tgcgtggtgc ggatctggtg gttaccgcaa tggcagaagg acgtcatgcg
    70981 gcacagggga ttattgactg gctgggggta aaatcagtca aatctcactg atagcctgcg
    71041 cagacaaaac cgacttcaca gcgtaagata attgttcatt tcgcgctgtg gagtcggtat
    71101 gacgcaacaa atcaccctca ttaaagacaa aattctctcc gataactatt tcaccctgca
    71161 caacattacc tacgatctca cgcgcaaaga tggcgaagtt atccgccata aacgtgaagt
    71221 ttacgatcgc ggtaatggtg cgacgatcct cctgtacaac gcgaagaaaa agagcgtggt
    71281 tctgattcgt cagttccgtg tcgctacctg ggttaatggc aatgaaagcg ggcagctgat
    71341 tgaaacctgc gccgggctgc tggataacga cgaaccggaa gtgtgcattc gtaaagaagc
    71401 gattgaagag acgggctatg aagtgggcga agtgcgcaaa ttatttgaac tgtatatgtc
    71461 gcctggcggt gtgactgagc taatccactt ttttatcgcc gaatacagtg acaatcagcg
    71521 cgctaacgcc ggtggcggtg ttgaagatga agatattgaa gtgctcgagc tgccgttcag
    71581 ccaggcgctg gagatgatca aaaccggcga gatacgtgac ggtaagacgg tgttattgct
    71641 taactatttg caaacgtcac atttaatgga ctgaaaaata acaataatat ttcgttgttt
    71701 attattggct caatccgaca aatctgattg agccgcgcta cctgcgcaac gaagatacga
    71761 cttgtgctgt ttgtttgaac ttctggggtc gtaccgtcca tgcgctatcg cattttcctt
    71821 ctcttttttt tcgctttgtt gccgacgtct ttggtgtggg cggcaccagc gcaacgggcg
    71881 ttttccgact ggcaggtcac ctgtaataac caaaacttct gcgtggcgcg caatacgggc
    71941 gatcataatg gactggtgat gaccctgagc cgcagcgccg gggcgcatac cgatgccgtt
    72001 ttacgtattg agcgcggcgg gttgaagtcg ccggatgcgt ctgaaggaga gatagcgcca
    72061 cggctgctgt tagatggtga gccgttggcg ttaagtggtg acaagtggcg gatttcacca
    72121 tggttattag taaccgatga tacggcaacc atcaccgcgt ttttgcagat gattcaggaa
    72181 gggaaggcga tcaccttacg cgacggcaat cagaccattt ctctgagtgg cttaaaagcg
    72241 gcgttgttgt ttattgatgc tcagcaaaag cgcgttggca gtgaaaccgc gtggatcaag
    72301 aaaggggacg aaccgccgct cagcgtaccg cccgcgcctg cgctgaaaga ggtcgcggtg
    72361 gttaacccaa cgccgacacc actctcactc gaagaacgca acgatttgct ggattatgga
    72421 aactggcgga tgaatggtct gcgctgctcg cttgatccgt tacgtcgtga ggtgaatgtc
    72481 actgcgctga ctgatgataa agcgctgatg atgattagct gtgaggcagg ggcctataac
    72541 accattgatt tggcatggat tgtgtcgcgt aaaaagccac tagcctcgcg cccggttcgg
    72601 ttgcgtttgc cgttcaacaa cggtcaggag acgaatgaac tggaactgat gaacgcaaca
    72661 tttgatgaga aatcgcgtga actggtgacc ttagcgaaag ggcgcggatt aagcgattgt
    72721 ggcattcagg cgcgctggcg ctttgacggt cagcgttttc gcctggtgcg ttatgccgca
    72781 gaacccacct gcgataactg gcatgggcca gacgcctggc ccacgttgtg gatcacccgt
    72841 taaacaaaat tgcggggaca tgtaggccgg ataagacgct gtaggccgga taagacgcgt
    72901 cagcgtcgca tccggcaatc agcatccggc gatcaccatc aggcaccttt cactcccagc
    72961 accttatgcg cttttgccac aatattctcg gcggtaaagc caaagaacgg gaacagctta
    73021 tccgccggag cagattcccc gtaacccgtc atcccgacaa ttgccccttt cagaccaaca
    73081 tacttgtacc agtaatcggc aatacctgct tccaccgcca cgcgagccgc aacgttagaa
    73141 ggcaacaccg actcccgata ttcctcatcc tgggcgtcga aaatatcggt cgagggcagg
    73201 gaaactacgc gtacattgcg accttctcct gctaatttct ctgccgcttg cagggtaatt
    73261 tccatctctg aaccggtggc aatcagaata atatctggct taccgccgct gtctttcagc
    73321 acataaccgc cacgagcaat ctctttaacc tgatccggcg tacgttccac ctgggccaga
    73381 ttctgccttg agaggatcag tgccgtcggt ccgttgtggc gctcaaccgc cagcttccag
    73441 cccaccgccg cttccacctg atcgcacggt cgccaggtgc tgaaatttgg cgttaagcgc
    73501 aggctggcca gttgctcaac agcctggtgc gtcggaccat cttcgcccag gccaattgag
    73561 tcgtgggtat aaaccataat ctgccgcgct ttcatcagtg ccgccatccg cgcggcgtta
    73621 cgggcgtatt caacaaacat caggaacgtc gcggtatacg gcacaaagcc gccgtggtgc
    73681 gcgatgccgt tggcgatagc ggtcatgcca aattcacgca ccccgtagtg aatgtagttg
    73741 cccgctggat cttccttcag cgaaacagaa cctttccaga tggtcaggtt gctgggagcc
    73801 agatccgccg aaccgccgag cagctccggt agcatcggcc cgtaagcgtt aagcgtattt
    73861 tgcgaagcct tacgggtagc gattttcgcc ggattcgcct gcaactcatt gatatatttc
    73921 tgagtcgttt tctcccagtc cttcggtaaa ccaccgctca tccgacgggt aaactcttct
    73981 gccagttgcg gatgagcctt tttataggcg gcaaacttct cattccagct ctgctgcgct
    74041 ttttcgcctt tttcgcgggc atcccaggcg tgataaatct ctttagggat ttcaaatggc
    74101 ggatggtgcc agcccagttt ttgccgtgcc aatgccactt cttcttcacc cagcggtgcg
    74161 ccatgcgcct cttccttacc cgctttgtta ggcgaaccga agccaattac tgtgcggcag
    74221 ataatcagcg acggcttatc tttcacgctt tgcgcttcaa ggatcgcttc cttcaccgcc
    74281 tgcggatcgt gaccgtcgat ttcatggatc acatgccagt gataggcttc aaaacgtttt
    74341 gccgtatcgt cggtaaacca gccttcagtt tcaccgtcga tggaaatacc gttgtgatcg
    74401 taaaaaccaa tcagcttgcc cagtcccagc gtgcctgcca gcgaacagac ttcgtgggaa
    74461 ataccttcca tcaggcagcc gtcgcccata aacacatagg tgaagtgatc gacgatctca
    74521 tggtctggct ggttaaactg cgccgccagt gtacgctctg ctatcgccag cccgacggcg
    74581 ttcgccaaac cttgtccaag cgggccggtg gtggtttcaa cgcctggcgt ataaccaatc
    74641 tccgggtggc ctggggtttt cgaatgcaac tgacggaagt tcttcagttc ttccagcggc
    74701 aggtcgtaac cggtcagatg tagcaaactg tagagcagca tcgacgcgtg accgttggaa
    74761 agaataaagc ggtcgcgatc ataccaggtt gggtcggtag ggttatgttt aagaaaatcg
    74821 ttccacagca cttcggcaat atcagccatg cccatcggcg cgccgggatg accagaattg
    74881 gctttttgta ccgcatccat actgagtgcg cgaatcgcat tggcaaggtc ttttcgggac
    74941 atataacact ccgtggctgg tttatagttt ggcggcaaga agatcttcca gtttgcgttg
    75001 atcaacggcg aacagacgaa tgccttcaga cagtttttct accgccatcg catcctgatt
    75061 gtgctcccaa cggaactccg cttcgctcat gggagctggg cgtgggaacg tctgagaagg
    75121 tgggattaat ttacgtacca ctggcgaaac tttttcctgc agctccttta gcaaattcgg
    75181 tgcgatagtc agtcgatcgc agccggttaa ggcgaggatt tgttcggtgc gacggaagct
    75241 cgcgcccatc acaatggttt catagtggtg ttgcttatag tagtcgtaga tattgcgcac
    75301 cgatttaacg cccggatctt cttccaccac atacgggtcc atcggcttgc gtgcctgata
    75361 ccagtcataa atacgcccga caaacggcga aatcagaaaa acgcccgctt ctgcacaggc
    75421 ccgcgcctgg gcaaaagaaa acagcagcgt caggttgcag ttaatacctt ctttttccag
    75481 ctcttcggcc gcgcgaattc cttcccaggt cgaagccagt ttgatcaaaa tgcgtgattt
    75541 ctcaacgcct tgttgctgat acaagtccac cagatggcgc gccttctcaa tactcttttc
    75601 tttatcaaaa gagaggcgtg catcaacttc tgttgacacg cgaccgggta cgattttgag
    75661 aatttcagca ccgaaattga ctgccagttt gtcacacgct gcgaccacct gttgttcctg
    75721 ggtcttgcca ttttttttac cccaggcgat agcatcgtct attaaatgct catattgtga
    75781 taatccggca gctttgagta acagcgaagg attggtggtg gcatcctggg gatgataatg
    75841 gcgaatggac tcaatatcgc cgctgtctgc cacgacagtg gtgaactgtt tgatgccgtc
    75901 taactcgttc ataggaaata ctccttgaaa agtaaagtgt tagatgagtg cgttaattca
    75961 cacttctgag aaatttcgct aaacgcatca aaaaagcata gcagacaggc atggtattgc
    76021 tggattaagc aggtaacatc agtgttatag gattattacc aaaacattat attaatggtg
    76081 agagtttgga cttgctcaaa gtctgtagac tccggcaggg taataatgtg cgccacgttg
    76141 tgggcagggg ctgttgccca cacactttat ttgtgaacgt tacgtgaaag gaacaaccaa
    76201 atggatgacc agttaaaaca aagtgcactt gatttccatg aatttccagt tccagggaaa
    76261 atccaggttt ctccaaccaa gcctctggca acgcagcgcg atctggcgct ggcctactca
    76321 ccaggcgttg ccgcaccttg tcttgaaatc gaaaaagacc cgttaaaagc ctacaaatat
    76381 accgcgcgcg gtaacctggt ggcggtgatc tctaacggta cggcggtgct ggggttaggc
    76441 aacattggcg cgctggctgg caaaccggtg atggaaggca agggcgttct gtttaagaaa
    76501 ttcgccggga ttgatgtatt tgacattgaa gttgatgaac tcgacccgga caaatttatt
    76561 gaagttgtcg ctgcgctcga accgaccttc ggcggaatca acctcgaaga cattaaagcg
    76621 ccagaatgtt tctatattga acagaaactg cgcgagcgga tgaatattcc ggtattccac
    76681 gacgatcagc acggcacggc aatcatcagc actgccgcca tcctcaacgg cttgcgcgtg
    76741 gtggagaaaa acatctccga cgtgcggatg gtggtttccg gcgcgggtgc cgcagcaatc
    76801 gcctgtatga acctgctggt agcgctgggt ctgcaaaaac ataacatcgt ggtttgcgat
    76861 tcaaaaggcg ttatctatca gggccgtgag ccaaacatgg cggaaaccaa agccgcatat
    76921 gcggtggtgg atgacggcaa acgtaccctc gatgatgtga ttgaaggcgc ggatattttc
    76981 cttggatgtt ccggcccgaa agtgctgacc caggaaatgg tgaagaaaat ggctcgtgcg
    77041 ccaatgatcc tggcgctggc gaacccggaa ccggaaattc tgccgccgct ggcgaaagaa
    77101 gtgcgtccgg atgccatcat ttgcaccggt cgttctgact atccaaacca ggtcaacaac
    77161 gtcctttgct tcccgtttat cttccgtggc gcgctggatg ttggcgcaac ggctatcaat
    77221 gaagagatga aactggcggc ggtacgcgcg attgcggaac tggcccacgc cgaacagagc
    77281 gaagtggtgg cttcagcgta tggcgatcag gatctgagct ttggtccgga atacatcatt
    77341 ccaaaaccgt ttgatccgcg cttgatcgtt aagatcgctc ctgcggtcgc taaagccgcg
    77401 atggagtcgg gcgtggcgac gcgtccgatt gctgatttcg acgtctacat cgacaagctg
    77461 actgagttcg tttacaaaac caacctgttt atgaagccga ttttctccca ggctcgcaaa
    77521 gcgccgaagc gcgttgttct gccagaaggg gaagaggcgc gcgttctgca tgccactcag
    77581 gaactggtaa cgctgggact ggcgaaaccg atccttatcg gtcgtccgaa cgtgatcgaa
    77641 atgcgcattc agaaactggg cttgcagatc aaagcgggcg ttgattttga gatcgtcaat
    77701 aacgaatccg atccgcgctt taaagagtac tggaccgaat acttccagat catgaagcgt
    77761 cgcggtgtca ctcaggaaca ggcgcagcgg gcgctgatca gtaacccgac agtgatcggc
    77821 gcgatcatgg ttcagcgtgg ggaagccgat gcaatgatct gcggtacggt gggcgattat
    77881 catgaacatt ttagcgtggt gaaaaatgtc tttggttatc gcgatggcgt tcacaccgca
    77941 ggtgcaatga acgcgctgct gctgccgagt ggtaacacct ttattgccga tacctacgtc
    78001 aatgacgaac cagacgcaga agagctggcg gagatcacct tgatggcggc agaaactgtc
    78061 cgtcgttttg gtattgagcc gcgcgttgct ctgttgtcgc actccaactt tggttcttct
    78121 gactgcccgt cgtcgagcaa aatgcgtcag gcgctggaac tggtcaggga tcgtgcgcca
    78181 gatctgatga ttgatggtga aatgcacggc gatgcagcgc tggtggaagc gattcgcaac
    78241 gaccgtatgc cggacagccc tttgaaaggt tctgccaata ttctggtgat gccgaacatg
    78301 gaagctgccc gcattagtta caacttactg cgcgtttcca gctcggaagg tgtgactgtc
    78361 ggtccggtgc tgatgggcgt ggcgaaaccg gttcacgtgt taacgccgat cgcatcggtg
    78421 cgtcgtatcg tcaacatggt ggcgctggcc gtggttgaag cgcaaaccca accgctgtaa
    78481 ttctttttta actctcacgc ttaccctgaa tattcagggt aagcggtttg gctgcaatat
    78541 attagtaaag cttattactg agtttgcgaa taataaaaaa gcagtctata taatatctcg
    78601 atattatttt atttatattc atgcgttgca tatgaaagtt tatgcaccac agcgaatatc
    78661 tctccattct tagtgatcta cctcaccttt taaacgcgct tgccgaattt tgttatttac
    78721 tctgacgaaa aattgtcacg atacacgaaa gtttttcaca ggcggcgact catggataaa
    78781 gaacgcatca ttcaggaatt tgtgcccggc aaacaggtca cactggcgca tctcatcgcg
    78841 catcccggtg aggaactggc gaagaaaatc ggcgttccgg atgcgggcgc aattggcatt
    78901 atgacgctaa ctcccggcga aacggcgatg atcgccggtg atttagcgct gaaagccgct
    78961 gatgtgcata tcggttttct cgaccgcttc agcggtgcat tggtgattta tggctcggtg
    79021 ggcgcagtag aagaagcgtt atcacaaacc gtcagcggtt taggtcgctt attaaattat
    79081 acgctttgtg aaatgaccaa aagttaatac gaggcattca tgaaacgaat tgcgtttgtg
    79141 ggtagcgtcg gggcaggaaa aacaacgtta tttaatgcgt tgcaggggaa ttataccctc
    79201 gccagaaaaa cacaggccgt ggaatttaat gataagggcg atattgatac gccaggtgaa
    79261 tattttagcc atccccgctg gtatcacgcc ttaattacca cgctacagga tgtagacatg
    79321 ctgatctatg tccatggcgc aaatgacccg gaaagtcggt tgcctgccgg gttgctggat
    79381 attggcgtca gcaaacggca aatcgccgtc atcagtaaaa cggacatgcc agatgctgac
    79441 gtcgccgcaa cgcgaaagtt attgcttgag actggcttcg aagagccgat tttcgagcta
    79501 aacagccacg acccgcaaag cgtgcagcaa ctggtggatt atctggcatc tctcaccaaa
    79561 caggaggaag caggtgaaaa aactcatcac agcgaatgat attcgtgaag cacacgcgcg
    79621 cggcgaacag gcaatgtccg tggttctgcg cgccagcatt attaccccgg aagcccgtga
    79681 agtggcggat ctactgggtt ttaccatcac cgaatgtgat gaatcgatcc cggtaacggc
    79741 gtccgtgccc gccagcgtgc cagccgacaa aaccgaaagc cagcgcatcc gcgaaaccat
    79801 cattgcccag ctgccggaag gccagtttac cgaaagcctg gtcgcacagc tgatggaaaa
    79861 agtgatgaag gaaaaacagt cgctggagca gggcgcaatg cagccgagct tcaaatcggt
    79921 gaccggcaaa ggcggcatca aagtgattga tggcagcagc gtcaagtttg gtcgtttcga
    79981 cggcgcagag ccgcattgcg tgggcttgac tgatcttgtg accggagacg acggcagcag
    80041 catggccgcc ggttttatgc agtgggagaa cgcattcttc ccgtggaccc tgaactacga
    80101 cgaaatcgac atggtgctgg aaggggagct acatgttcgc cacgaaggcg aaaccatgat
    80161 cgccaaagca ggcgacgtga tgtttatccc gaaaggctcc agcatcgaat ttggtacgac
    80221 atccagcgtg aaattcctgt atgtcgcctg gccggctaac tggcaatccc tatgaaagat
    80281 ttcatcaccg aagcatggct aagagcgaac catacgctca gcgaaggcgc agagatccat
    80341 ctccctgcgg acagtcgcct gacgccctct gcccgggagt tgctggaaag ccgccatctg
    80401 cgcatcaagt ttattgacga gcagggccgc ctgtttgttg acgatgaaca gcagcagccg
    80461 cagcccgttc atgggctgac cagtagcgat gaacatccgc aggcgtgctg cgaactgtgc
    80521 cgccagccgg tggcaaaaaa gccggatacg ctgactcacc tgtcggcgga aaaaatggtc
    80581 gccaaaagcg atccgcgtct gggttttcgc gcggttctcg acagcaccat tgcgctggca
    80641 gtgtggctgc aaattgaact ggcggaaccg tggcagccgt ggctagcgga tatccgttcg
    80701 cgtcttggca acattatgcg cgccgatgcg ctgggggaac cgctgggctg ccaggcgatt
    80761 gtcgggcttt ctgacgaaga tctgcatcgg ctttctcatc agccgctgcg ctatctcgac
    80821 cacgatcatc tggtgccgga agccagccac gggcgcgatg cggcgttgct caatctgcta
    80881 cgtaccaaag tgcgggaaac cgaaaccgta gcggcgcagg tgtttatcac ccgcagcttt
    80941 gaagtattac gcccggacat tttgcaggcg ctgaaccgcc tttcgagcac ggtctacgtg
    81001 atgatgatcc tgagcgtaac gaagcagccg ctaacggtga agcagatcca acaacgactg
    81061 ggggaaacgc aatgattatt gaacgttgtc gtgaactggc gttgcgagcg cccgccagag
    81121 tggtttttcc ggatgcgtta gaccaacgtg tgctgaaagc cgcgcaatat ttacatcaac
    81181 aaggtctggc aacgcccatt ctggtcgcca atccgtttga acttcgtcag tttgcgctca
    81241 gtcacggcgt ggcgatggac gggctacagg tgattgatcc gcatggcaac ctcgcaatgc
    81301 gggaagaatt tgctcatcgc tggctggccc gcgcgggcga aaaaacgccg ccggatgcgc
    81361 tggaaaaact taccgaccct ctgatgttcg ccgcagcaat ggtcagcgcc ggtaaagcgg
    81421 atgtctgtat cgcgggcaac ctctcttcca cggcaaatgt gctgcgtgcc ggattacgca
    81481 ttatcggctt gcagccaggc tgtaaaacgc tctcttccat tttcctgatg ttgccacagt
    81541 acagcggccc ggcgttgggc tttgccgatt gcagcgtggt gccacagccg acggcggcgc
    81601 agctggcgga tatcgcgctt gccagtgccg aaacctggcg cgccatcacc ggagaagagc
    81661 cgcgcgtggc gatgctgtcg ttttccagca atggtagcgc ccgtcacccc tgtgttgcta
    81721 acgtccagca ggcgacagaa atcgtccgtg agcgcgcacc aaagctggtg gtggatggcg
    81781 agttacagtt tgacgccgcc ttcgtgccgg aagtggcggc gcaaaaagcg cctgccagcc
    81841 cgctacaggg caaggccaat gtgatggttt ttccgtcgct ggaagccgga aatattggtt
    81901 acaaaatcgc acaacgactc ggcggatatc gtgccgtcgg gccactgata caaggacttg
    81961 ccgcgccgat gcacgatctc tctcgtggtt gtagcgtgca ggaaattatc gagctggcgc
    82021 tggtggcagc tgtgccgcgt cagacagaag tgaaccgcga aagcagttta caaacactgg
    82081 ttgaatgaac ggtcccgttc tggaccctga taaagaggaa agcacaatgg aagcattagg
    82141 aatgatcgaa acccggggcc tggttgcact gattgaggct tctgacgcga tggtgaaagc
    82201 tgcgcgcgtc aagctggttg gtgttaagca gattggcggc ggcctgtgta ctgccatggt
    82261 tcgtggtgat gttgcagcct gtaaagccgc gaccgatgca ggtgccgcag cggcacagcg
    82321 tatcggcgag ctggtatctg tacacgttat cccgcgtccg cacggcgacc tggaagaagt
    82381 cttcccgatc ggcctgaaag gcgatagcag caacctgtaa tcaccctgtg tgacgtcgat
    82441 tcgggcgtca cgctctctcc tttttcattt taccttctgc ggagggtagg gggagctttt
    82501 acctgaaata cgccacggag gcgggtatga aactggcagt cgtcactgga caaattgttt
    82561 gtaccgtacg ccatcacgga ctggcgcatg acaaattgct gatggtggaa atgattgatc
    82621 cacaaggtaa tcctgacggg caatgcgccg tcgccatcga caatattggc gcgggaaccg
    82681 gggagtgggt gttactggtg agtggcagtt ccgcccgcca ggcgcataaa agcgaaacgt
    82741 caccggtcga tctgtgcgtg attggcattg tcgatgaggt ggtgtctggc ggtcaggtaa
    82801 ttttccacaa ataaggcaga acatcatgaa tcaacaggat attgaacagg tggtgaaagc
    82861 ggtactgctg aaaatgcaaa gcagtgacac gccgtccgcc gccgttcatg agatgggcgt
    82921 tttcgcgtcc ctggatgacg ccgttgcggc agccaaagtc gcccagcaag ggttaaaaag
    82981 cgtggcaatg cgccagttag ccattgctgc cattcgtgaa gcaggcgaaa aacacgccag
    83041 agatttagcg gaacttgccg tcagtgaaac cggcatgggg cgcgttgaag ataaatttgc
    83101 caaaaacgtc gctcaggcgc gcggcacacc aggcgttgag tgcctctctc cgcaggtgct
    83161 gaccggcgac aacggcctga ctttaattga aaacgcgccc tggggcgtgg tggcttcggt
    83221 aactccgtcc actaacccgg cggcaaccgt aattaataac gccatcagcc tgattgccgc
    83281 gggcaacagc gtcatttttg ccccgcatcc ggcggcgaaa aaagtctccc agcgggcgat
    83341 cacgctgctc aaccaggcga ttgttgccgc aggcgggccg gaaaacttac tggttactgt
    83401 ggcaaatccg gatatcgaaa ccgcgcagcg tttgttcaag tttccgggca tcggccttct
    83461 ggtggtgacc ggcggcgaag cggtagtaga agcggcgcgt aaacacacca ataaacgtct
    83521 gattgccgca ggcgctggca acccgccggt agtggtggat gaaaccgccg acctcgcccg
    83581 tgccgctcag tccatcgtca aaggcgcttc tttcgataac aacatcattt gtgccgacga
    83641 aaaggtactg attgttgttg atagcgtagc cgatgaactg atgcgtctga tggaaggcca
    83701 gcacgcggtg aaactgaccg cagaacaggc gcagcagctg caaccggtgt tgctgaaaaa
    83761 tatcgacgag cgcggaaaag gcaccgtcag ccgtgactgg gttggtcgcg atgcggcgaa
    83821 aatcgcggcg gcaatcggcc tgaacgtgcc gcaagaaacg cgtttgctgt ttgtggaaac
    83881 cactgcagaa catccgtttg ccgtgaccga actgatgatg ccggtgctgc ccgtcgtgcg
    83941 cgtcgccaac gtggcggatg ccattgcgct ggcggtgaaa ctggaaggcg gttgccacca
    84001 cacggcggca atgcactcgc gcaacatcga aaacatgaac cagatggcga atgctattga
    84061 taccagcatt ttcgttaaga a
//
LOCUS       DAZGAN020000006        81633 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGAN020000006 DAZGAN020000000
VERSION     DAZGAN020000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 81633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.006"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..431)
                     /locus_tag="ACHKYO_RS02330"
                     /old_locus_tag="pgaptmp_000466"
     CDS             complement(<1..431)
                     /locus_tag="ACHKYO_RS02330"
                     /old_locus_tag="pgaptmp_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HGU4427364.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRG"
     gene            complement(591..803)
                     /gene="rpmE"
                     /locus_tag="ACHKYO_RS02335"
                     /old_locus_tag="pgaptmp_000467"
     CDS             complement(591..803)
                     /gene="rpmE"
                     /locus_tag="ACHKYO_RS02335"
                     /old_locus_tag="pgaptmp_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HGU4427365.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1006..3204
                     /gene="priA"
                     /locus_tag="ACHKYO_RS02340"
                     /old_locus_tag="pgaptmp_000468"
     CDS             1006..3204
                     /gene="priA"
                     /locus_tag="ACHKYO_RS02340"
                     /old_locus_tag="pgaptmp_000468"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGU4427366.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3360..4385
                     /gene="cytR"
                     /locus_tag="ACHKYO_RS02345"
                     /old_locus_tag="pgaptmp_000469"
     CDS             3360..4385
                     /gene="cytR"
                     /locus_tag="ACHKYO_RS02345"
                     /old_locus_tag="pgaptmp_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HGU4427367.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4477..5436
                     /gene="ftsN"
                     /locus_tag="ACHKYO_RS02350"
                     /old_locus_tag="pgaptmp_000470"
     CDS             4477..5436
                     /gene="ftsN"
                     /locus_tag="ACHKYO_RS02350"
                     /old_locus_tag="pgaptmp_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HGU4427368.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5529..6059
                     /gene="hslV"
                     /locus_tag="ACHKYO_RS02355"
                     /old_locus_tag="pgaptmp_000471"
     CDS             5529..6059
                     /gene="hslV"
                     /locus_tag="ACHKYO_RS02355"
                     /old_locus_tag="pgaptmp_000471"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HGU4427369.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6069..7400
                     /gene="hslU"
                     /locus_tag="ACHKYO_RS02360"
                     /old_locus_tag="pgaptmp_000472"
     CDS             6069..7400
                     /gene="hslU"
                     /locus_tag="ACHKYO_RS02360"
                     /old_locus_tag="pgaptmp_000472"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HGU4427370.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7467..8393
                     /gene="menA"
                     /locus_tag="ACHKYO_RS02365"
                     /old_locus_tag="pgaptmp_000473"
     CDS             7467..8393
                     /gene="menA"
                     /locus_tag="ACHKYO_RS02365"
                     /old_locus_tag="pgaptmp_000473"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HGU4427371.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8486..8971
                     /gene="rraA"
                     /locus_tag="ACHKYO_RS02370"
                     /old_locus_tag="pgaptmp_000474"
     CDS             8486..8971
                     /gene="rraA"
                     /locus_tag="ACHKYO_RS02370"
                     /old_locus_tag="pgaptmp_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HGU4427372.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(9056..9301)
                     /gene="zapB"
                     /locus_tag="ACHKYO_RS02375"
                     /old_locus_tag="pgaptmp_000475"
     CDS             complement(9056..9301)
                     /gene="zapB"
                     /locus_tag="ACHKYO_RS02375"
                     /old_locus_tag="pgaptmp_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HGU4427373.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9726..10571
                     /gene="glpF"
                     /locus_tag="ACHKYO_RS02380"
                     /old_locus_tag="pgaptmp_000476"
     CDS             9726..10571
                     /gene="glpF"
                     /locus_tag="ACHKYO_RS02380"
                     /old_locus_tag="pgaptmp_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HGU4427374.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10594..12102
                     /gene="glpK"
                     /locus_tag="ACHKYO_RS02385"
                     /old_locus_tag="pgaptmp_000477"
     CDS             10594..12102
                     /gene="glpK"
                     /locus_tag="ACHKYO_RS02385"
                     /old_locus_tag="pgaptmp_000477"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGU4427375.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12237..13247
                     /gene="glpX"
                     /locus_tag="ACHKYO_RS02390"
                     /old_locus_tag="pgaptmp_000478"
     CDS             12237..13247
                     /gene="glpX"
                     /locus_tag="ACHKYO_RS02390"
                     /old_locus_tag="pgaptmp_000478"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU4427376.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13344..14090
                     /gene="fpr"
                     /locus_tag="ACHKYO_RS02395"
                     /old_locus_tag="pgaptmp_000479"
     CDS             13344..14090
                     /gene="fpr"
                     /locus_tag="ACHKYO_RS02395"
                     /old_locus_tag="pgaptmp_000479"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HGU4427377.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(14095..14523)
                     /gene="uspD"
                     /locus_tag="ACHKYO_RS02400"
                     /old_locus_tag="pgaptmp_000480"
     CDS             complement(14095..14523)
                     /gene="uspD"
                     /locus_tag="ACHKYO_RS02400"
                     /old_locus_tag="pgaptmp_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HGU4427378.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14550..14849)
                     /gene="yiiS"
                     /locus_tag="ACHKYO_RS02405"
                     /old_locus_tag="pgaptmp_000481"
     CDS             complement(14550..14849)
                     /gene="yiiS"
                     /locus_tag="ACHKYO_RS02405"
                     /old_locus_tag="pgaptmp_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HGU4427379.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(15061..15501)
                     /gene="yiiR"
                     /locus_tag="ACHKYO_RS02410"
                     /old_locus_tag="pgaptmp_000482"
     CDS             complement(15061..15501)
                     /gene="yiiR"
                     /locus_tag="ACHKYO_RS02410"
                     /old_locus_tag="pgaptmp_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4427380.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15602..16201
                     /gene="yiiQ"
                     /locus_tag="ACHKYO_RS02415"
                     /old_locus_tag="pgaptmp_000483"
     CDS             15602..16201
                     /gene="yiiQ"
                     /locus_tag="ACHKYO_RS02415"
                     /old_locus_tag="pgaptmp_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HGU4427381.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16309..17076
                     /gene="tpiA"
                     /locus_tag="ACHKYO_RS02420"
                     /old_locus_tag="pgaptmp_000484"
     CDS             16309..17076
                     /gene="tpiA"
                     /locus_tag="ACHKYO_RS02420"
                     /old_locus_tag="pgaptmp_000484"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGU4427382.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17131..17886)
                     /gene="cdh"
                     /locus_tag="ACHKYO_RS02425"
                     /old_locus_tag="pgaptmp_000485"
     CDS             complement(17131..17886)
                     /gene="cdh"
                     /locus_tag="ACHKYO_RS02425"
                     /old_locus_tag="pgaptmp_000485"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HGU4427383.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(17993..18982)
                     /gene="sbp"
                     /locus_tag="ACHKYO_RS02430"
                     /old_locus_tag="pgaptmp_000486"
     CDS             complement(17993..18982)
                     /gene="sbp"
                     /locus_tag="ACHKYO_RS02430"
                     /old_locus_tag="pgaptmp_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HGU4427384.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGMFDQISKR"
     gene            complement(19302..20264)
                     /gene="pfkA"
                     /locus_tag="ACHKYO_RS02435"
                     /old_locus_tag="pgaptmp_000487"
     CDS             complement(19302..20264)
                     /gene="pfkA"
                     /locus_tag="ACHKYO_RS02435"
                     /old_locus_tag="pgaptmp_000487"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGU4427385.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20445..21347)
                     /gene="fieF"
                     /locus_tag="ACHKYO_RS02440"
                     /old_locus_tag="pgaptmp_000488"
     CDS             complement(20445..21347)
                     /gene="fieF"
                     /locus_tag="ACHKYO_RS02440"
                     /old_locus_tag="pgaptmp_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HGU4427386.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21496..21996)
                     /gene="cpxP"
                     /locus_tag="ACHKYO_RS02445"
                     /old_locus_tag="pgaptmp_000489"
     CDS             complement(21496..21996)
                     /gene="cpxP"
                     /locus_tag="ACHKYO_RS02445"
                     /old_locus_tag="pgaptmp_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HGU4427387.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            22146..22844
                     /gene="cpxR"
                     /locus_tag="ACHKYO_RS02450"
                     /old_locus_tag="pgaptmp_000490"
     CDS             22146..22844
                     /gene="cpxR"
                     /locus_tag="ACHKYO_RS02450"
                     /old_locus_tag="pgaptmp_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HGU4427388.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22841..24214
                     /gene="cpxA"
                     /locus_tag="ACHKYO_RS02455"
                     /old_locus_tag="pgaptmp_000491"
     CDS             22841..24214
                     /gene="cpxA"
                     /locus_tag="ACHKYO_RS02455"
                     /old_locus_tag="pgaptmp_000491"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HGU4427389.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24320..24994)
                     /gene="yiiM"
                     /locus_tag="ACHKYO_RS02460"
                     /old_locus_tag="pgaptmp_000492"
     CDS             complement(24320..24994)
                     /gene="yiiM"
                     /locus_tag="ACHKYO_RS02460"
                     /old_locus_tag="pgaptmp_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HGU4427390.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(25143..26126)
                     /gene="kdgT"
                     /locus_tag="ACHKYO_RS02465"
                     /old_locus_tag="pgaptmp_000493"
     CDS             complement(25143..26126)
                     /gene="kdgT"
                     /locus_tag="ACHKYO_RS02465"
                     /old_locus_tag="pgaptmp_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HGU4427391.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26386..27006)
                     /gene="sodA"
                     /locus_tag="ACHKYO_RS02470"
                     /old_locus_tag="pgaptmp_000494"
     CDS             complement(26386..27006)
                     /gene="sodA"
                     /locus_tag="ACHKYO_RS02470"
                     /old_locus_tag="pgaptmp_000494"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HGU4427392.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27291..28325
                     /gene="rhaT"
                     /locus_tag="ACHKYO_RS02475"
                     /old_locus_tag="pgaptmp_000495"
     CDS             27291..28325
                     /gene="rhaT"
                     /locus_tag="ACHKYO_RS02475"
                     /old_locus_tag="pgaptmp_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HGU4427393.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGLSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGIAN"
     gene            complement(28322..29170)
                     /gene="rhaR"
                     /locus_tag="ACHKYO_RS02480"
                     /old_locus_tag="pgaptmp_000496"
     CDS             complement(28322..29170)
                     /gene="rhaR"
                     /locus_tag="ACHKYO_RS02480"
                     /old_locus_tag="pgaptmp_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HGU4427394.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(29244..30080)
                     /gene="rhaS"
                     /locus_tag="ACHKYO_RS02485"
                     /old_locus_tag="pgaptmp_000497"
     CDS             complement(29244..30080)
                     /gene="rhaS"
                     /locus_tag="ACHKYO_RS02485"
                     /old_locus_tag="pgaptmp_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HGU4427395.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30368..31837
                     /gene="rhaB"
                     /locus_tag="ACHKYO_RS02490"
                     /old_locus_tag="pgaptmp_000498"
     CDS             30368..31837
                     /gene="rhaB"
                     /locus_tag="ACHKYO_RS02490"
                     /old_locus_tag="pgaptmp_000498"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HGU4427396.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNMVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFIPNPDSEIAHYLAQIHSTRQTKELCA"
     gene            31834..33093
                     /gene="rhaA"
                     /locus_tag="ACHKYO_RS02495"
                     /old_locus_tag="pgaptmp_000499"
     CDS             31834..33093
                     /gene="rhaA"
                     /locus_tag="ACHKYO_RS02495"
                     /old_locus_tag="pgaptmp_000499"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HGU4427397.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
     gene            33335..34159
                     /gene="rhaD"
                     /locus_tag="ACHKYO_RS02500"
                     /old_locus_tag="pgaptmp_000500"
     CDS             33335..34159
                     /gene="rhaD"
                     /locus_tag="ACHKYO_RS02500"
                     /old_locus_tag="pgaptmp_000500"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HGU4427398.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            34169..34483
                     /gene="rhaM"
                     /locus_tag="ACHKYO_RS02505"
                     /old_locus_tag="pgaptmp_000501"
     CDS             34169..34483
                     /gene="rhaM"
                     /locus_tag="ACHKYO_RS02505"
                     /old_locus_tag="pgaptmp_000501"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HGU4427399.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34796..35242
                     /gene="frvA"
                     /locus_tag="ACHKYO_RS02510"
                     /old_locus_tag="pgaptmp_000502"
     CDS             34796..35242
                     /gene="frvA"
                     /locus_tag="ACHKYO_RS02510"
                     /old_locus_tag="pgaptmp_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HGU4427400.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
     gene            35253..36704
                     /gene="frvB"
                     /locus_tag="ACHKYO_RS02515"
                     /old_locus_tag="pgaptmp_000503"
     CDS             35253..36704
                     /gene="frvB"
                     /locus_tag="ACHKYO_RS02515"
                     /old_locus_tag="pgaptmp_000503"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HGU4427401.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSHLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYVLAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36694..37764
                     /gene="frvX"
                     /locus_tag="ACHKYO_RS02520"
                     /old_locus_tag="pgaptmp_000504"
     CDS             36694..37764
                     /gene="frvX"
                     /locus_tag="ACHKYO_RS02520"
                     /old_locus_tag="pgaptmp_000504"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4427402.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAAFKNCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            37764..39512
                     /gene="frvR"
                     /locus_tag="ACHKYO_RS02525"
                     /old_locus_tag="pgaptmp_000505"
     CDS             37764..39512
                     /gene="frvR"
                     /locus_tag="ACHKYO_RS02525"
                     /old_locus_tag="pgaptmp_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HGU4427403.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(39562..40602)
                     /gene="yiiG"
                     /locus_tag="ACHKYO_RS02530"
                     /old_locus_tag="pgaptmp_000506"
     CDS             complement(39562..40602)
                     /gene="yiiG"
                     /locus_tag="ACHKYO_RS02530"
                     /old_locus_tag="pgaptmp_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HGU4427404.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVVVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALRE
                     YNEMVDDYNSLR"
     gene            complement(40755..41588)
                     /gene="fdhD"
                     /locus_tag="ACHKYO_RS02535"
                     /old_locus_tag="pgaptmp_000507"
     CDS             complement(40755..41588)
                     /gene="fdhD"
                     /locus_tag="ACHKYO_RS02535"
                     /old_locus_tag="pgaptmp_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HGU4427405.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            41782..44832
                     /gene="fdnG"
                     /locus_tag="ACHKYO_RS02540"
                     /old_locus_tag="pgaptmp_000508"
     CDS             41782..44832
                     /gene="fdnG"
                     /locus_tag="ACHKYO_RS02540"
                     /old_locus_tag="pgaptmp_000508"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:42367..42369,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU4427406.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            44845..45747
                     /gene="fdoH"
                     /locus_tag="ACHKYO_RS02545"
                     /old_locus_tag="pgaptmp_000509"
     CDS             44845..45747
                     /gene="fdoH"
                     /locus_tag="ACHKYO_RS02545"
                     /old_locus_tag="pgaptmp_000509"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HGU4427407.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            45744..46379
                     /gene="fdoI"
                     /locus_tag="ACHKYO_RS02550"
                     /old_locus_tag="pgaptmp_000510"
     CDS             45744..46379
                     /gene="fdoI"
                     /locus_tag="ACHKYO_RS02550"
                     /old_locus_tag="pgaptmp_000510"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU4427408.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            46376..47305
                     /gene="fdhE"
                     /locus_tag="ACHKYO_RS02555"
                     /old_locus_tag="pgaptmp_000511"
     CDS             46376..47305
                     /gene="fdhE"
                     /locus_tag="ACHKYO_RS02555"
                     /old_locus_tag="pgaptmp_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HGU4427409.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(47635..47877)
                     /gene="yiiF"
                     /locus_tag="ACHKYO_RS02560"
                     /old_locus_tag="pgaptmp_000512"
     CDS             complement(47635..47877)
                     /gene="yiiF"
                     /locus_tag="ACHKYO_RS02560"
                     /old_locus_tag="pgaptmp_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HGU4427410.1"
                     /translation="MNSLVGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(48097..48315)
                     /gene="yiiE"
                     /locus_tag="ACHKYO_RS02565"
                     /old_locus_tag="pgaptmp_000513"
     CDS             complement(48097..48315)
                     /gene="yiiE"
                     /locus_tag="ACHKYO_RS02565"
                     /old_locus_tag="pgaptmp_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGU4427411.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKRGRQRGETVGVYDQCKDHKE"
     gene            complement(48901..49191)
                     /gene="nadS"
                     /locus_tag="ACHKYO_RS02570"
                     /old_locus_tag="pgaptmp_000514"
     CDS             complement(48901..49191)
                     /gene="nadS"
                     /locus_tag="ACHKYO_RS02570"
                     /old_locus_tag="pgaptmp_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HGU4427412.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(49192..49503)
                     /locus_tag="ACHKYO_RS02575"
                     /old_locus_tag="pgaptmp_000515"
     CDS             complement(49192..49503)
                     /locus_tag="ACHKYO_RS02575"
                     /old_locus_tag="pgaptmp_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4427413.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            49732..50640
                     /locus_tag="ACHKYO_RS02580"
                     /old_locus_tag="pgaptmp_000516"
     CDS             49732..50640
                     /locus_tag="ACHKYO_RS02580"
                     /old_locus_tag="pgaptmp_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4427414.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(50704..51645)
                     /gene="fabY"
                     /locus_tag="ACHKYO_RS02585"
                     /old_locus_tag="pgaptmp_000517"
                     /gene_synonym="yiiD"
     CDS             complement(50704..51645)
                     /gene="fabY"
                     /locus_tag="ACHKYO_RS02585"
                     /old_locus_tag="pgaptmp_000517"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HGU4427415.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(51690..52127)
                     /gene="dtd"
                     /locus_tag="ACHKYO_RS02590"
                     /old_locus_tag="pgaptmp_000518"
     CDS             complement(51690..52127)
                     /gene="dtd"
                     /locus_tag="ACHKYO_RS02590"
                     /old_locus_tag="pgaptmp_000518"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGU4427416.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(52124..52996)
                     /gene="yihY"
                     /locus_tag="ACHKYO_RS02595"
                     /old_locus_tag="pgaptmp_000519"
     CDS             complement(52124..52996)
                     /gene="yihY"
                     /locus_tag="ACHKYO_RS02595"
                     /old_locus_tag="pgaptmp_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HGU4427417.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(52990..53589)
                     /gene="yihX"
                     /locus_tag="ACHKYO_RS02600"
                     /old_locus_tag="pgaptmp_000520"
     CDS             complement(52990..53589)
                     /gene="yihX"
                     /locus_tag="ACHKYO_RS02600"
                     /old_locus_tag="pgaptmp_000520"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HGU4427418.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(53730..54617)
                     /locus_tag="ACHKYO_RS02605"
                     /old_locus_tag="pgaptmp_000521"
     CDS             complement(53730..54617)
                     /locus_tag="ACHKYO_RS02605"
                     /old_locus_tag="pgaptmp_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HGU4427419.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(54607..55956)
                     /locus_tag="ACHKYO_RS02610"
                     /old_locus_tag="pgaptmp_000522"
     CDS             complement(54607..55956)
                     /locus_tag="ACHKYO_RS02610"
                     /old_locus_tag="pgaptmp_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4427420.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(56055..57487)
                     /locus_tag="ACHKYO_RS02615"
                     /old_locus_tag="pgaptmp_000523"
                     /pseudo
     CDS             complement(56055..57487)
                     /locus_tag="ACHKYO_RS02615"
                     /old_locus_tag="pgaptmp_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(57557..58567)
                     /locus_tag="ACHKYO_RS02620"
                     /old_locus_tag="pgaptmp_000524"
     CDS             complement(57557..58567)
                     /locus_tag="ACHKYO_RS02620"
                     /old_locus_tag="pgaptmp_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HGU4427421.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(58600..59487)
                     /locus_tag="ACHKYO_RS02625"
                     /old_locus_tag="pgaptmp_000525"
     CDS             complement(58600..59487)
                     /locus_tag="ACHKYO_RS02625"
                     /old_locus_tag="pgaptmp_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HGU4427422.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(59512..60390)
                     /gene="yihU"
                     /locus_tag="ACHKYO_RS02630"
                     /old_locus_tag="pgaptmp_000526"
     CDS             complement(59512..60390)
                     /gene="yihU"
                     /locus_tag="ACHKYO_RS02630"
                     /old_locus_tag="pgaptmp_000526"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HGU4427423.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPCKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            60563..61459
                     /locus_tag="ACHKYO_RS02635"
                     /old_locus_tag="pgaptmp_000527"
     CDS             60563..61459
                     /locus_tag="ACHKYO_RS02635"
                     /old_locus_tag="pgaptmp_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU4427424.1"
                     /translation="MTKIACVGITVQDRIYSLLTLPDGGGKYQENHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            61499..62287
                     /locus_tag="ACHKYO_RS02640"
                     /old_locus_tag="pgaptmp_000528"
     CDS             61499..62287
                     /locus_tag="ACHKYO_RS02640"
                     /old_locus_tag="pgaptmp_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4427425.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(62398..63663)
                     /gene="yihN"
                     /locus_tag="ACHKYO_RS02645"
                     /old_locus_tag="pgaptmp_000529"
     CDS             complement(62398..63663)
                     /gene="yihN"
                     /locus_tag="ACHKYO_RS02645"
                     /old_locus_tag="pgaptmp_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4427426.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(63765..64745)
                     /gene="yihM"
                     /locus_tag="ACHKYO_RS02650"
                     /old_locus_tag="pgaptmp_000530"
     CDS             complement(63765..64745)
                     /gene="yihM"
                     /locus_tag="ACHKYO_RS02650"
                     /old_locus_tag="pgaptmp_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGU4427427.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(64753..65463)
                     /gene="yihL"
                     /locus_tag="ACHKYO_RS02655"
                     /old_locus_tag="pgaptmp_000531"
     CDS             complement(64753..65463)
                     /gene="yihL"
                     /locus_tag="ACHKYO_RS02655"
                     /old_locus_tag="pgaptmp_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4427428.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLATLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(65680..67503)
                     /gene="typA"
                     /locus_tag="ACHKYO_RS02660"
                     /old_locus_tag="pgaptmp_000532"
     CDS             complement(65680..67503)
                     /gene="typA"
                     /locus_tag="ACHKYO_RS02660"
                     /old_locus_tag="pgaptmp_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HGU4427429.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            67876..69285
                     /gene="glnA"
                     /locus_tag="ACHKYO_RS02665"
                     /old_locus_tag="pgaptmp_000533"
     CDS             67876..69285
                     /gene="glnA"
                     /locus_tag="ACHKYO_RS02665"
                     /old_locus_tag="pgaptmp_000533"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HGU4427430.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            69458..70507
                     /gene="glnL"
                     /locus_tag="ACHKYO_RS02670"
                     /old_locus_tag="pgaptmp_000534"
     CDS             69458..70507
                     /gene="glnL"
                     /locus_tag="ACHKYO_RS02670"
                     /old_locus_tag="pgaptmp_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HGU4427431.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            70519..71928
                     /gene="glnG"
                     /locus_tag="ACHKYO_RS02675"
                     /old_locus_tag="pgaptmp_000535"
     CDS             70519..71928
                     /gene="glnG"
                     /locus_tag="ACHKYO_RS02675"
                     /old_locus_tag="pgaptmp_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HGU4427432.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            72040..72150
                     /gene="yshB"
                     /locus_tag="ACHKYO_RS02680"
                     /old_locus_tag="pgaptmp_000536"
     CDS             72040..72150
                     /gene="yshB"
                     /locus_tag="ACHKYO_RS02680"
                     /old_locus_tag="pgaptmp_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HGU4427433.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(72340..73713)
                     /gene="hemN"
                     /locus_tag="ACHKYO_RS02685"
                     /old_locus_tag="pgaptmp_000537"
     CDS             complement(72340..73713)
                     /gene="hemN"
                     /locus_tag="ACHKYO_RS02685"
                     /old_locus_tag="pgaptmp_000537"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HGU4427434.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(73902..74411)
                     /gene="yihI"
                     /locus_tag="ACHKYO_RS02690"
                     /old_locus_tag="pgaptmp_000538"
     CDS             complement(73902..74411)
                     /gene="yihI"
                     /locus_tag="ACHKYO_RS02690"
                     /old_locus_tag="pgaptmp_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HGU4427435.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(74530..74696)
                     /locus_tag="ACHKYO_RS02695"
                     /old_locus_tag="pgaptmp_000539"
                     /pseudo
     CDS             complement(74530..74696)
                     /locus_tag="ACHKYO_RS02695"
                     /old_locus_tag="pgaptmp_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            74993..75625
                     /gene="yihA"
                     /locus_tag="ACHKYO_RS02700"
                     /old_locus_tag="pgaptmp_000540"
     CDS             74993..75625
                     /gene="yihA"
                     /locus_tag="ACHKYO_RS02700"
                     /old_locus_tag="pgaptmp_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGU4427436.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(76007..78793)
                     /gene="polA"
                     /locus_tag="ACHKYO_RS02705"
                     /old_locus_tag="pgaptmp_000541"
     CDS             complement(76007..78793)
                     /gene="polA"
                     /locus_tag="ACHKYO_RS02705"
                     /old_locus_tag="pgaptmp_000541"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGU4427437.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            79157..80089
                     /gene="yihG"
                     /locus_tag="ACHKYO_RS02710"
                     /old_locus_tag="pgaptmp_000542"
     CDS             79157..80089
                     /gene="yihG"
                     /locus_tag="ACHKYO_RS02710"
                     /old_locus_tag="pgaptmp_000542"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGU4427438.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(80130..81560)
                     /gene="yihF"
                     /locus_tag="ACHKYO_RS02715"
                     /old_locus_tag="pgaptmp_000543"
     CDS             complement(80130..81560)
                     /gene="yihF"
                     /locus_tag="ACHKYO_RS02715"
                     /old_locus_tag="pgaptmp_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU4427439.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT        20139 a        21134 c        20458 g        19902 t
ORIGIN      
        1 ccgcgcacca gccacagtga ctcgctgcgg ctgtaaccgt cctcaccggg aaacaggtgc
       61 tcaaaataca ggcttctgcc gccgttatca ctgagtatca gtgtgttatc gcgcagctgt
      121 aagcggatat ccgcaggcat tttccagccg gggccgaggc tccccaccgg cgcgggcgtt
      181 tttgtccggt aactgctgta ggtgcgggag aggatgaacg gcagcggggc gggcagggcg
      241 atgtcggttt caccgggaag gacctttgca ccgagcaggg gattgaccgg atggccggac
      301 gtcactccgc cggggcacac cgaacaggcc acgccggtgg gggcaccgat gcgcaccccg
      361 gctgaaccct ggacaatgct accgccatac tgcgtcatgt cgccctgacg cgctgccggt
      421 tttccgctca tcgtaaaatc cttttgaaat aaataagaac cagaagccta gcgccagtca
      481 acaacaggaa atgataatga gttaacaccc cggaattttt cagaatttat aaaaggcaaa
      541 ttacaggcac aaaaaaagcg ccgtgcggcg cttttttcgg aaatccggtc ttatttgctg
      601 cccgggatgt tgaaacgctt gttgaagcgg tcaacacggc caccggtagc aacatcacgc
      661 tgtttgccag tgaagaacgg gtggcacttg ctgcacacgt cgaggttcag gtcatgacca
      721 acggtggagc ggattttcat tacgttaccg caagagcagc tagcagtaat ttcttcgtat
      781 ttcgggtgaa tatctttttt catgggaaaa cctcggttta aggccgcgtc gctcttccag
      841 ccctaacgcc agacaccacg cgatgttaaa agtatagctt caatacgatc atttcgtacg
      901 aaggcgcgaa atcatacaga aattaaccag cgtatgcaaa ctgatccgca ctcttctacg
      961 gcaatgtgta tactaaccca ccgaatttca agtcaggatg atgctatgcc cgttgcccac
     1021 gttgccttgc ccgttccgct tcctcgtacc tttgactatc tgctgccaga aggcatgacg
     1081 gttaaagccg ggtgtcgcgt gcgcgtgccg tttggcaaac agcaggagcg catcggggtt
     1141 gtggtgtcag ttagcgatgt cagtgagctg ccgcttaacg agctgaaagc ggtagtggaa
     1201 gtgctggacg ttgagccggt atttactcac tccgtctggc ggttactgct ctgggcggca
     1261 gattactatc atcatccgat tggcgatgtg ctgtttcatg ccttgccgat tttactgcgc
     1321 caggggcggc ctgcggcgaa cgcgccgatg tggtactggt ttgccactga acaaggccag
     1381 gcggtggatc tgaacagcct gaaacgctcc cccaagcaac aacaggcgct ggcggcgtta
     1441 cggcaaggca aaatctggcg cgaccaggtc gccacgctcg aatttaatga tgccgcgttg
     1501 caggcgctac gcaaaaaagg tctgtgtgat ttagcaagtg aaacaccaga gtttagcgac
     1561 tggcgaacga actatgccgt ttctggtgag cggttgcgat tgaataccga acaagccacc
     1621 gccgttggcg caattcatag cgcggcagat actttttctg cctggctgct ggcgggcgtt
     1681 accggttccg gtaaaacgga ggtttatctc agtgtactgg aaaacgtgct cgctcagggc
     1741 aaacaggcgc tggtgatggt gccggaaatc ggcctgacac cgcaaactat cgcccgtttt
     1801 cgtgaacgtt ttaatgcccc cgtggaagtt ctgcattccg gcctgaacga cagcgagcgg
     1861 ctttcggcgt ggttgaaagc gaaaaatggc gaggcggcga ttgtgatcgg cacccgctcc
     1921 gcgctgttta cgccgtttaa aaatctcggc gtgattgtca ttgatgaaga gcacgacagc
     1981 tcctacaagc agcaggaagg ctggcgctat catgcccgcg acctggcggt gtatcgtgcg
     2041 catagcgagc aaatcccgat tattcttggc tccgcaacgc ccgcgctgga aacgttatgc
     2101 aacgtccagc agaaaaaata ccgcctgctg cgcctgaccc gtcgggcagg gaatgcgcgt
     2161 ccggcaattc aacatgtgct ggatttaaaa ggtcagaagg tgcaggcagg tctggctccg
     2221 gcgttaatca cccgtatgcg ccagcattta caggccaaca atcaggttat cctctttctt
     2281 aatcgtcggg gttttgcgcc tgcgctgctg tgccacgact gcggctggat agccgaatgc
     2341 ccacgttgcg atcactacta cacgctgcat caggcgcaac agcatttgcg ctgtcaccac
     2401 tgtgacagtc agcgtccggt gccgcgccag tgcccttcct gcggttccac gcacctggtc
     2461 cccgtggggc tgggcaccga acagcttgaa cagacgcttg cgccgatgtt ccccggcgtg
     2521 cccatttcac gtatcgaccg cgataccacc agccgcaaag gggcgctgga acagcaactg
     2581 gcggaagtgc atcgcggcgg cgcgaggatc ttgattggca cgcaaatgct ggcgaaaggt
     2641 caccactttc cggatgtgac gctggtggcg ttactggatg tggacggcgc gctgttttct
     2701 gccgattttc gctcggcaga acgtttcgcc cagctttaca cccaggtcgc cggtcgtgcc
     2761 gggcgtgcgg gtaaacaggg cgaagtggtg ctgcaaacgc accatccgga acatcctctg
     2821 ttgcaaacgt tgctctataa aggctacgac gcctttgccg aacaggcgct ggctgagcga
     2881 cgcatgatgc agctgccgcc gtggaccagc catgtgattg tgcgtgcgga agatcataac
     2941 aatcagcatg cgccgctgtt cctgcaacaa ctgcgtaatc tgatcctttc cagcccgctg
     3001 gcagacgaca aactgtgggt tctcggcccg gttccggctc tggcacctaa gcgtggtggc
     3061 cgctggcgct ggcagatctt gttgcagcac ccttcccgcg tgcgcttgca acacatcatt
     3121 agtggtacgc tggcgcttat taatacaata ccggattccc gcaaggtgaa atgggtgttg
     3181 gatgtcgatc ccatcgaagg ctaatgcgca aacgttgcga ggcggatcga aaaattcaat
     3241 attcatcaca cttttcatga aaattctgta accgttttca cgcgctatct gctaaaaatg
     3301 ttgccgatgt gaagtaaaca tggatgtagt acgcctgacg tgccaggcga ggagtgagtg
     3361 tgaaagcgaa gaagcaggaa actgccgcga ccatgaaaga cgttgccctc aaggcaaaag
     3421 tctctacagc gactgtctcc cgagcattaa tgaatcccga taaagtctcc caggccaccc
     3481 gtaatcgggt tgaaaaagcg gcccgggaag tgggttattt accgcaacct atggggcgca
     3541 acgtcaagcg taatgaatcc cgcaccattc tggtgattgt cccggatatc tgcgatccct
     3601 tctttagcga aattattcgc ggtatcgaag ttacggcggc aaatcacgga tatctggtgc
     3661 tgattggcga ctgtgcgcat caaaatcagc aggaaaaaac ctttatcgat ttgatcatca
     3721 ccaagcaaat tgatggcatg ttgctgctgg gctcaaggct gccgtttgat gccagcattg
     3781 aggaacagcg taatctgccg ccgatggtga tggcgaacga atttgcaccg gaactggagc
     3841 tgcctaccgt tcatatcgac aatctgaccg ccgcatttga tgcagtaaat tatttatatg
     3901 agcaagggca taaacggatt ggctgtatag ccggtcccga agagatgcca ctgtgtcact
     3961 accgcctgca aggctatgtt caggcgctgc gtcgctgcgg cattatggtt gatccgcaat
     4021 acatcgcccg tggcgacttc accttcgaag ccggaagcaa agcgatgcag cagctgcttg
     4081 atcttccaca accgcctact gctgtcttct gccatagcga tgtgatggcg ctcggcgcac
     4141 tttctcaggc aaaacgccag gggctgaaag tcccggaaga cctttccata atcggttttg
     4201 ataacatcga cctgacgcaa ttttgtgatc cgccgctgac aaccatcgcg cagccgcgtt
     4261 acgaaatcgg tcgggaagct atgctgttat tgcttgatca aatgcagggg caacacgttg
     4321 gcagtggctc tcgtttaatg gactgcgaac ttatcatccg gggatcaaca cgcgcgttac
     4381 cttaaagtaa acctttaaga ctgccgtgac tggtcaaagg cccgccgctt aagtaacatg
     4441 gcgggctgac gaacgaataa atacagcgaa acgatagtgg cacaacgaga ttatgtacgc
     4501 cgcagccaac cggcaccttc gcggcgaaaa aagagcacct cacggaaaaa gcaacgaaat
     4561 ctgcctgcgg tttctcccgc tatggtcgct attgctgccg ccgttcttgt gacctttatc
     4621 ggtggtctgt acttcattac gcatcacaag aaagaagagt ccgagacgct gcaaagccag
     4681 aaagtgaccg gtaacggact accaccaaaa ccagaagaac gctggcgcta cattaaagag
     4741 ctggaaagtc gccagccggg tgtgcgtgcg cccacagaac cttctgccgg tggtgaagtg
     4801 aaaacgccgg agcaactgac accagaacaa cgtcagcttc ttgaacaaat gcaggctgat
     4861 atgcgccagc agccaacgca gctggttgaa gttccgtgga atgaacagac gccggaacag
     4921 cgtcagcaaa cgctacaacg ccagcgtcag gcgcagcaat tagcggagca gcaacgcctg
     4981 gctcagcagt cgcgtacgac tgaacaaagc tggcagcagc agacgcgtac gtcgcaagcc
     5041 gcgccggtac aggcgcaacc gcgccagtct aaaccggctt ctactcagca gccgtaccag
     5101 gatctgctgc aaacccctgc gcacacgacc gcgcagtcga agccgcagca agctgcgccg
     5161 gttgctcgtg ccgctgacgc gccaaaaccg acggcggaga aaaaagacga acgccgctgg
     5221 atggtgcagt gcggttcgtt cagaggcgcg gaacaggcag agacggtacg tgctcagctg
     5281 gcgttcgaag gatttgactc gaaaatcacc accaacaatg gctggaatcg tgtggtcatt
     5341 ggcccggtga aaggcaaaga gaacgcagac agcaccctca atcggttgaa gatggcgggt
     5401 catacaaact gcattcggct cgccgccggg ggttgaaacc ctcaaaatcc cccccatcta
     5461 taattgcatt atgccccgta cttttgtacg gggtttgtac tctgtattcg taaccaaggg
     5521 gtcagctcgt gacaactata gtaagcgtac gccgtaacgg ccatgtggtc atcgctggtg
     5581 atggtcaggc cacgttgggc aataccgtaa tgaaaggcaa cgtgaaaaag gtccgccgtc
     5641 tgtacaacga caaagtcatc gcgggctttg cgggcggtac tgcggatgct tttacgctgt
     5701 tcgaactgtt tgaacgtaaa ctggaaatgc atcagggcca tctggtcaaa gccgccgttg
     5761 agctggcgaa agactggcgt accgatcgca tgctgcgcaa acttgaagca ctgctggcag
     5821 tcgcggatga aactgcatcg cttatcatca ccggtaacgg tgacgtggtg cagccagaaa
     5881 acgatcttat tgctatcggc tccggcggcc cttacgccca ggctgcggcg cgtgcactgt
     5941 tagaaaatac tgaactgagt gctcgtgaaa ttgctgaaaa ggcgttggat attgcaggcg
     6001 acatttgcat ctataccaac catttccaca ccatcgaaga attaagctac aaagcgtaag
     6061 gatctcccat gtctgaaatg accccacgcg aaatcgtcag cgaactggat aagcacatca
     6121 tcggccagga caacgccaag cgttctgtgg cgattgctct gcgtaaccgc tggcgtcgca
     6181 tgcagctcaa cgaagagctg cgccatgaag tgaccccgaa aaatatcctg atgatcggcc
     6241 cgaccggtgt cggtaaaact gaaatcgccc gtcgtctggc taagctggcg aatgcgccgt
     6301 tcatcaaagt tgaagcgacc aaattcaccg aagtgggcta cgtcggtaag gaagtggatt
     6361 ctattattcg cgatctgacc gatgccgccg tgaaaatggt acgcgtccag gctatcgaga
     6421 aaaaccgtta tcgcgctgaa gaactggcag aagaacgtat tctcgacgtg ctgatcccac
     6481 ctgctaaaaa caactgggga cagaccgaac agcagcagga accgtccgct gctcgtcagg
     6541 cattccgcaa aaaactgcgt gaaggccagc ttgatgacaa agaaatcgag atcgatcttg
     6601 ccgcagcacc gatgggcgtt gaaattatgg ctcctccagg catggaagag atgaccagcc
     6661 agctgcagtc catgttccag aacctgggcg gccagaagca aaaagcgcgt aagctgaaaa
     6721 tcaaagacgc catgaagctg ctgattgaag aagaagcggc gaaactggtg aacccggaag
     6781 agctgaagca agacgctatc gacgctgttg agcagcacgg gatcgtgttt atcgacgaaa
     6841 tcgacaaaat ctgtaagcgc ggcgagtctt ccggtccgga tgtttctcgt gaaggcgttc
     6901 agcgtgacct gctgccgctg gtagaaggtt gcaccgtttc caccaaacac gggatggtca
     6961 aaactgacca cattctgttt atcgcttctg gcgcgttcca gattgcgaaa ccgtctgacc
     7021 tgatcccgga actgcaaggt cgtctgccaa tccgcgttga actgcaggcg ctgaccacca
     7081 gcgacttcga gcgtattctg accgagccga atgcctctat caccgtgcag tacaaagcac
     7141 tgatggcgac tgaaggcgta aatatcgagt ttaccgactc cggtattaag cgcatcgcgg
     7201 aagcggcatg gcaggtgaac gaatctaccg aaaacatcgg tgctcgtcgt ttacacactg
     7261 ttctggagcg tttaatggaa gagatttcct acgacgccag cgatttaagc ggtcaaacta
     7321 tcatcattga cgcagattat gtgagcaaac atctggatgc gttggtggca gatgaagatc
     7381 tgagccgttt tatcctataa tcgcgttcaa tcattttcat cattgtttga tggggctgaa
     7441 aggccccatt tttattggcg cgtattatga ctgaacaaca aattagccga actcaggcgt
     7501 ggctggaaag tttacgacct aaaaccctcc ccctcgcctt tgctgcaatt atcgtcggga
     7561 cagcgctggc atggtggcaa ggtcacttcg atccgctggt cgccctgctg gcactgatta
     7621 ccgccgggct attacagatc ctttctaacc tcgccaatga ttacggcgat gcggtaaaag
     7681 gcagcgataa acctgaccgc attgggccgc tacgcggcat gcaaaaaggg gtcattaccc
     7741 agcaagagat gaaacgggcg ctcattatta ccgtcgtgct catctgtctc tccgggctgg
     7801 cactggttgc agtggcatgc catacgctgg ccgattttgt cggtttcctg attcttggcg
     7861 ggttgtcgat cattgccgct atcacctaca ccgtgggcaa tcgtccttat ggttatatcg
     7921 gtctgggtga tatttccgta ctggttttct ttggctggtt gagtgtcatg gggagctggt
     7981 atttacaggc tcatacattg attccggcac tgatccttcc ggcgaccgca tgcggcctgc
     8041 tggcaacggc agtactgaat attaataacc tgcgtgatat caatagcgac cgcgaaaatg
     8101 gcaaaaacac gctggtggtg cgcttaggtg aagtgaacgc gcgtcgttat catgcctgcc
     8161 tgctgatggg ctcgctggtg tgtctggcgc tgtttaatct cttttcgctg catagcctgt
     8221 ggggctggct gttcctgctg gcggcaccat tactggtgaa gcaagcccgt tatgtgatgc
     8281 gggaaatgga cccggtggcg atgcgaccaa tgctggaacg tactgtcaag ggagcgttac
     8341 tgactaacct gctgtttgtt ttagggatat tcctaagcca gtgggcagca taactgacaa
     8401 atatcaatta acaattgatg attttgccaa cagcctacat agcgcgatat actgaaaatt
     8461 ctcgcagcaa ctgaatgtta agcctatgaa atacgatact tccgagcttt gtgacatcta
     8521 tcaagaagat gttaacgtcg tggaaccgct gttctccaac tttggcggac gggcgtcgtt
     8581 tggcggacaa ataatcacgg taaaatgttt cgaggacaac gggttgctgt acgatctgct
     8641 cgaacagaat ggccgtggtc gtgttcttgt cgtcgatggc ggtggttctg ttcgtcgcgc
     8701 actggtcgat gctgaactgg cgcgtctggc agtacaaaat gaatgggaag gtctggtcat
     8761 ttacggcgcg gtgcgtcagg tagatgacct ggaagagttg gatatcggca tccaggcgat
     8821 ggcggcaatt ccggttggtg ccgctggcga aggcattggc gaaagcgatg tccgcgtcaa
     8881 ttttggcggt gtcaccttct tctccggcga ccatctttat gccgacaata ccgggattat
     8941 tctttctgaa gatccgctgg atattgaatg atagaaaagg caccgcaggg tgcctttttt
     9001 ctgcgttacc tgttggccta cacagtaaag aaattacgcg gaagatgaag cgtaatcaga
     9061 cctcttccat gcgacccagc agggcctgca gacgttcctg ccagccgttc tgctgttctt
     9121 tcagatggtt gttctcacgc tccagctctt cgcgctgatg ctgggcattt tgaacttcct
     9181 gcgacagtga gttgtttttt tctttcagct cttcgatttc catctgcaac agagtgatgg
     9241 tatcaatcgc ctgctgtact tttgcttcca gtttctcaaa cacttctaat gacattgtca
     9301 tacctctcct gaattgcaag gcgttgatgg ataaaaatcc tcgtcccgat taccggtgac
     9361 gccttaataa atacgagcgc actttagtta gctccgattg tatgaagccg cgccatcgct
     9421 gtccagcggc acgccttgca gattacggtt tgccacactt ttcatccttc tcctggtgac
     9481 ataatctaca tcaatcgaaa atgttaataa atttgttgcg cgaatgatct aacaaacatg
     9541 catcatgtac aatcagatgg aataaatggc gcgataacgc tcattttatg acgaagcaca
     9601 cacattttaa gttcgatatt tctcgttttt gctcgttaac gataagttta cagcatgcct
     9661 acaagcatcg tggaggtccg tgactttcac gcatacaaca aacattaact cttcaggatc
     9721 cgattatgag tcaaacatca accttgaaag gccagtgcat tgctgaattc ctcggtaccg
     9781 ggttgttgat tttcttcggt gtgggttgcg ttgcagcact aaaagtcgct ggtgcgtctt
     9841 ttggtcagtg ggaaatcagt gtcatttggg gactgggggt ggcaatggcc atctacctga
     9901 ccgcaggggt ttccggcgcg catcttaatc ccgctgttac cattgcattg tggctgtttg
     9961 cctgtttcga caagcgcaaa gttattcctt ttatcgtttc acaagttgcc ggcgctttct
    10021 gtgctgcggc tttagtttac gggctttact acaatttatt tttcgacttc gagcagactc
    10081 atcacattgt tcgcggcagc gttgaaagtg ttgatctggc tggcactttc tctacttacc
    10141 ctaatcctca tatcaatttt gtgcaggctt tcgcagttga gatggtgatt accgctattc
    10201 tgatggggct gatcctggcg ttaacggacg atggcaacgg tgtaccacgc ggccctttgg
    10261 ctcccttgct gattggtcta ctgattgcgg tcattggcgc atctatgggc ccattgacag
    10321 gttttgccat gaacccagcg cgtgacttcg gtccgaaagt ctttgcctgg ctggcgggct
    10381 ggggcaatgt cgcctttacc ggcggcagag acattcctta cttcctggtg ccgcttttcg
    10441 gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg caaactgatt ggtcgccatt
    10501 tgccttgcga tatctgtgtt gtggaagaaa aggaaaccac aactccttca gaacaaaaag
    10561 cttcgctgta atatgactac gggacaatta aacatgactg aaaaaaaata tatcgttgcg
    10621 ctcgaccagg gcaccaccag ctcccgcgcg gtcgtaatgg atcacgatgc caatatcatt
    10681 agcgtgtcgc agcgcgaatt tgagcaaatc tacccaaaac caggttgggt agaacacgac
    10741 ccaatggaaa tctgggccac ccaaagctcc acgctggtag aagtgctggc gaaagccgat
    10801 atcagttccg atcaaattgc agctatcggt attacgaacc agcgtgaaac cactattgtc
    10861 tgggaaaaag aaaccggcaa gcctatctat aacgccattg tctggcagtg ccgtcgtacc
    10921 gcagaaatct gcgagcattt aaaacgtgac ggtttagaag attatatccg cagcaatacc
    10981 ggtctggtga ttgacccgta cttttctggc accaaagtga agtggatcct cgaccatgtg
    11041 gaaggctctc gcgagcgtgc acgtcgtggt gaattgctgt ttggtacggt tgatacgtgg
    11101 cttatctgga aaatgactca gggccgtgtc catgtgaccg attacaccaa cgcctctcgt
    11161 accatgttgt tcaacatcca taccctggac tgggacgaca aaatgctgga agtgctggat
    11221 attccgcgcg agatgctgcc agaagtgcgt cgttcttccg aagtatacgg tcagactaac
    11281 attggcggca aaggcggcac gcgtattcca atctccggga tcgccggtga ccagcaggcc
    11341 gcgctgtttg gtcagttgtg cgtgaaagaa gggatggcga agaacaccta tggcactggc
    11401 tgctttatgc tgatgaacac tggcgagaaa gcggtgaaat cagaaaacgg cctgctgacc
    11461 accatcgcct gcggcccgac tggcgaagtg aactatgcgt tggaaggtgc ggtgtttatg
    11521 gcaggcgcat ccattcagtg gctgcgcgat gagatgaagt tgattaacga cgcctacgat
    11581 tccgaatatt tcgccaccaa agtgcaaaac accaatggtg tgtatgtggt tccggcattt
    11641 accgggctgg gtgcgccgta ctgggacccg tatgcgcgcg gggcgatttt cggtctgact
    11701 cgtggggtga acgctaacca cattatccgc gcgacgctgg agtctattgc ttatcagacg
    11761 cgtgacgtgc tggaagcgat gcaggccgac tctggtatcc gtctgcacgc cctgcgcgtg
    11821 gatggtggcg cagtagcaaa caatttcctg atgcagttcc agtccgatat tctcggcacc
    11881 cgcgttgagc gcccggaagt gcgcgaagtc accgcattgg gtgcggccta tctcgcaggc
    11941 ctggcggttg gcttctggca gaacctcgac gagctgcaag agaaagcggt gattgagcgc
    12001 gagttccgtc caggcatcga aaccactgag cgtaattacc gttacgcagg ctggaaaaaa
    12061 gcggttaaac gcgcgatggc gtgggaagaa cacgacgaat aatgtaaatg ccgaatgaag
    12121 cgtttatgcc gcatccggta gtcccgaaac gtgcgggggc aaccccgcac acatcaataa
    12181 tccctccctt cccctgtgct acacttcgcg ccattcctta ctgcttagag tttgctatga
    12241 gacgagaact tgccatcgaa ttttcccgcg tcaccgaatc agcggcgctg gctggctaca
    12301 aatggttagg acgcggcgat aaaaacaccg cggacggcgc ggcggtaaac gccatgcgta
    12361 ttatgctcaa ccaggtcaac attgacggca ccatcgtcat tggtgaaggt gaaatcgacg
    12421 aagcaccgat gctctacatt ggtgaaaaag tcggtactgg tcgcggcgac gcggtagata
    12481 ttgctgttga tccgattgaa ggcacgcgca tgacggcgat gggccaggct aacgcgctgg
    12541 cggtgctggc agtaggcgat aaaggctgct tcctcaatgc gccggatatg tatatggaga
    12601 agctgattgt cgggccggga gccaaaggca ccattgatct gaacctgccg ctggcggata
    12661 acctgcgcaa tgtagcggcg gcgctcggca aaccgttgag cgaactgacg gtaacgattc
    12721 tggctaaacc acgccacgat gccgttatcg ctgaaatgca gcaactcggc gtacgcgtat
    12781 ttgctattcc ggatggcgac gttgcggcct caattctcac ctgtatgcca gacagcgaag
    12841 ttgacgtgct gtacggtatt ggtggcgcgc cggaaggcgt agtttctgcg gcggtgatcc
    12901 gcgcattaga tggcgacatg aacggtcgtc tgctggcgcg tcatgacgtc aaaggcgaca
    12961 acgaagagaa tcgtcgcatt ggcgagcagg agctggcacg ctgcaaagcg atgggcatcg
    13021 aagccggtaa agtattgcgc ctgggcgata tggcgcgcag cgataacgtc atcttctctg
    13081 ccaccggtat taccaaaggc gatctgctgg aaggcattag ccgcaaaggc aatatcgcga
    13141 ctaccgaaac gctgctgatc cgcggcaagt cacgcaccat tcgccgcatt cagtccatcc
    13201 actatctgga tcgcaaagac ccggaaatgc aggtgcacat cctctgattg atttgatcga
    13261 ttgagccttc cagtccttcg ggactggaat ttttttgttc ggagaacgaa gataaggcaa
    13321 gtcaatcaaa acaggagaaa aacatggctg attgggtaac aggcaaagtc actaaagtgc
    13381 agaactggac cgacgccctg tttagtctca ccgttcacgc ccccgtgctt ccgtttaccg
    13441 ccgggcaatt caccaagctt ggccttgaaa tcgacggcga acgcgtccag cgcgcctact
    13501 cctatgttaa ctcgcccgat aatcccgatc tggagtttta cctggtcacc gtccccgatg
    13561 gcaaattaag cccacgattg gcggcactga aaccaggcga tgaagtgcag gtggttagcg
    13621 aagcagctgg cttctttgtt ctggatgaag taccggactg cgaaacgcta tggatgctgg
    13681 caaccggtac agcgattggc ccttatttat cgattctgca actaggcaaa gatttagatc
    13741 gcttcaaaaa tctggtcctg gttcacgccg cacgttatgc cgccgactta agctatttgc
    13801 cactgatgca agaactggaa aaacgctacg aagggaaact gcgcattcag acggtggtca
    13861 gtcgggaaac ggcagcgggg tcgctcaccg ggcggatacc ggcgttaatt gaaagtgggg
    13921 aactggaaag cgcgattggc ctgccgatga ataaagaaac cagccatgtg atgctgtgcg
    13981 gcaatccaca gatggtgcgc gatacacaac agttgctgaa agagacccgg cagatgacga
    14041 aacatttacg tcgccgaccg ggccatatga cagcggagca ttactggtaa gcggttactt
    14101 atcgataaac ggcacgatga gcaaatccgc actcatctta ttgatcatcc cgcgatatgc
    14161 cggcatcaaa cggttgataa atgagtgatg atgaccacag acaaggaggt cgcactgctc
    14221 tttttgcata atttccagca gtgtttccgg catttctccg cgttcaatac gcagttttgt
    14281 cttcggccat tgaatatttt tcgtcagttt atacagcttg ttatccgact tattcttcaa
    14341 caattgaaga atatcttctg ttgcagggaa gtagataccc ggatacaact cgcttaagcc
    14401 atcatcaata tgaattaacg tcaggtgagc gtcattatgt ctggcgagct ccagagcttt
    14461 attcaccagt aaggcatctt cttcattccc ggaaattgcc acgccaatgt gtttataagc
    14521 catgtttaac tccttctaaa gcaactccat caagctaacg aacgcagtga taactcaaaa
    14581 ataatcatct cggcctggca tgagaaagtg aaggccgcat caagctcaac ttgcccctcc
    14641 agatccttaa tcttgtaggc cacgctggcc ccttcatctg cagaggatgc agcggcggca
    14701 agctctttca gtttggtcat aaaagactct gcttcttcgc gggtggcaaa aaagcgcgaa
    14761 aacttactgg tacagttgtc gttatcaatc accgtaccga tatcaatcgc acatccttta
    14821 gtactgcatt tatctacgac atctttcata gggcacctct gtatattcgc ctctctgtcg
    14881 attacgactt aagaataatg cgccgttaag ggaagaacaa acaagtaatg atcttatgga
    14941 ttaccatttt ttttgatatt gcttaaggtg aagagttcag aaggattttc cgggtattgt
    15001 catttgcatc gtggaaataa caatcccgga aaaaaatagc agtaagagat atattaaagg
    15061 ttaattactt gatttattgt cggctttata cttcacatcc tgagtatctt taccatattt
    15121 attttcgcct tgtgtgccaa caaacgcacc aagatcgata agcatcatca ccagaatcaa
    15181 cgtcgggaca aaacgcccca ccgcccattg ccagacaccc ggtaaaatcg cccagttacc
    15241 cgccagcagc atccacgcca caatcatcag aaatgcccat gcgccggaac gcccgcgatc
    15301 atgcaagcgc ttaacagtta ctgccgctgt tggccaaagc aagcacacaa ggcaaaacgc
    15361 cgcggtctga atatcgagta aatttttacc cgccagtgag aaaagcacca gcatgcctgc
    15421 gaaccacagg cctatccaaa tccagaaatc acggcgtcca atacgccctt taaatgagaa
    15481 taaccattgc tgtatggtca tgtaagttcc ttgatggttg tcttttccag gattctaccc
    15541 ttttgacaag ggcgacagat atcgttttaa tcggagccag tcataacaaa aggtactgtc
    15601 aatgaagcca gggtgtacgc tgttttttct cttatgttct gcattaaccg ttacaacaac
    15661 ggcgcatgca caaacaccag atacggcaac gaccgcgcct tatctgctgg ctggagcccc
    15721 tactttcgat ctctccatca gccagtttcg agaagacttt aacagccaga atcctagcct
    15781 gccactgaac gaatttcgtg ccatcgacag cagtcccgac aaagccaatc tcactcgtgc
    15841 tgcaagtaaa attaacgaga acttgtatgc ttctacagcg ctggagcgcg gtaccttaaa
    15901 aatcaaaagc attcaaatga cctggctacc catccagggg ccagagcaaa aagcagcgaa
    15961 agcgaaagct caggaataca tggcagcagt gatccgcaca ctcaccccat taatgaccaa
    16021 aacacaaagc cagaaaaaac tgcagtcgct gctaacggcg gggaaaaaca aacgttatta
    16081 caccgagaca gaaggtgcac tgcgttatgt tgtcgcggac aacggcgaaa aggggctgac
    16141 cttcgctgtt gaaccgatta agctggcgct atctgaatcg cttgaaggtt tgaataaatg
    16201 acaaaaagca aagcctttgt gccgatgaat ctctatactg tttcacagac ctgctgccct
    16261 gcggggcggc catcttcctt tattcgctta taagcgtgga gaattaaaat gcgacatcct
    16321 ttagtgatgg gtaactggaa actgaacggc agccgccaca tggttcacga gctggtttct
    16381 aacctgcgta aagagctggc aggtgttgct ggctgtgcgg ttgcaatcgc accaccggaa
    16441 atgtacatcg atatggcgaa gcgcgaagct gaaggcagcc acatcatgct gggtgcgcaa
    16501 aacgtggacc tgaacctgtc cggcgcattc accggtgaaa cttctgccgc tatgctgaaa
    16561 gacatcggcg ctcagtacat catcatcggc cactctgaac gtcgtactta ccacaaagag
    16621 tctgacgaac tgatcgcgaa aaaattcgcg gtgctgaaag agcagggcct gactccggtt
    16681 ctgtgcatcg gtgaaaccga agctgaaaac gaagcgggca aaactgaaga agtttgcgca
    16741 cgtcagatcg acgcggtact gaaaactcag ggtgctgcgg cattcgaagg tgcggttatc
    16801 gcttacgaac ctgtatgggc aatcggtact ggcaaatctg caactccggc tcaggcacag
    16861 gctgttcaca aattcatccg tgaccacatc gctaaagttg acgctaacat cgctgaacaa
    16921 gtgatcattc agtacggcgg ctctgtaaac gcgtctaacg ctgcagaact gtttgctcag
    16981 ccagatatcg acggcgcgct ggttggcggt gcttctctga aagctgacgc tttcgcagtg
    17041 atcgttaaag ctgcagaagc ggctaaacag gcttaagtct gacaggtgcc ggatttcata
    17101 tccggcactt actttcctta actcctcgcc ttaacgcaaa atctcacact catgatcctg
    17161 aatttcctcg gctgaagcac ggttaagcgt cagtagattt cgttgtgtcg ccagcaatac
    17221 aaatgagtta tcactctgcc gtaccatcgc cagcccgtag cttcccatat gttcccgcgc
    17281 ctcaggtact tcttctgcca gcatcataaa tgggctgcgt tgtaccagtt cgctttccgt
    17341 tacccgacgc gccaggtatt catgcccgcg caaaccacct ggcagtggca accagcggct
    17401 gctgatgttc gccagattgt tatccagttg ttcgcgcaca tcaggacgaa tacaagagat
    17461 atgaatatga aaatggtttt gcgtacgccc ggtgcgggag ttgatcgcca aagaaaccgc
    17521 gcgatcggga accggctggc cgtatttttt gctcataaaa tcacgcgcct gccaggccaa
    17581 ccaaaagaag ttcggcgttg aaggatcggt caacaaagga ctttcagtac cgttaatacg
    17641 atacgttggc atcaacagat attgcagtgg accattaaga tcttttaaaa ccacgtatcc
    17701 ggcattgggt ttgacttccg cacatggcga aggattttga ttttgctgct gattgggcaa
    17761 acattcctca aggacaatct tacgtaatgt atccgactct tcaccggtta atttccagta
    17821 accaataccg gcagccacaa cggcgataac tatcatcacc aaaaaaagaa gacccgcttt
    17881 tttcatcttt ttttccctgt acctcaaaga gatgcaaggg taacgcaaaa tcgtgacaaa
    17941 taaaaaaccc ggtcgcgaga aaccaccggg tttgataatt atcctgggga aatcagcgtt
    18001 tgctgatctg atcgaacata ccgccgttag caaaatgctc tttttgcgct ttcgtccagc
    18061 cgccgaactc ttcatcaatg gtgaataact tcagctttgg aaacgcattt tcgtactttt
    18121 tcgccacctc agcgtcgcgc ggacggtagt agtttttcgc ggcaatttcc tgaccttctg
    18181 gcgagtagag atatttcagg taggcttccg ccacctcttt ggtgcctttt ttctcgacca
    18241 ctttatcgac caccgacacg gttggctctg cgaggataga ctcactcggc gtgacgattt
    18301 cgaatttatc tttccccagt tcattcgctg ccagcagagc ttcgttttcc caggcgatca
    18361 gtacatcgcc aatcccgcgc tcgacaaagg tgttagtgga gccgcgcgcg ccagaatcca
    18421 gaacttcgac gtttttatac agtgcccgaa caaaatcctg tgcttttgcc tgatcgttgt
    18481 tgttgtgatg cagcgcgtag ccccaggcag ccaggtagtt ccagcgtgcg ccaccagagc
    18541 ttttcggatt aggcgtgatc accgaaacac ccggtttaat cagatcgttc cagtcatgga
    18601 tctgcttcgg attgccctta cgcaccagga aaacaatggt ggaagtgtac ggtgcggagt
    18661 tatccggcag acgtttgatc cactctttat caatccgccc gcgttccgca attgcgtcca
    18721 cgtcataggc cagagccagc gtgacaacat cagcttcaat accgttgatt accgacgtcg
    18781 cttgtttacc tgaaccaccg tgtgactgac gaatcaccac gttatcacca gtttgctgtt
    18841 tccagtgggc gctgaatgcc ttgttgtact gttcgtacaa ttcgcgcgtt ggatcatatg
    18901 aaacgttaag aagctgaata tcctttgcca taacgctggt tgccgccagc aaaaatgtta
    18961 accctacgcc ccacttgttc atcgcccgac tctcttatgt tgtgttgtga tgagcaaagc
    19021 gtgccagaag gttaaccaaa cattaaagaa taaaaaaaga ttggctataa cttgcgggta
    19081 tatgttgaag gattaaaaag gcggaagaaa tccgcctcat attgctgaca aagtccgctt
    19141 tgtccatgcc ggatgcggcg cgaacgcctt atccggccta caaaagtttg caaattcaat
    19201 aaattgcaga attaatgtag gcctgataag cgaagcgcat caggcatttt tgcttctgtc
    19261 atcggtttca ggctaaatga atctgccttt ttccgaaatc attaatacag ttttttcgcg
    19321 cagtccagcc agtcgccttt gaacggacgc ttcatgtttt cgatagcgtc gatgatgtcg
    19381 tggtgaacca actgttcgtt ctggatacct acgcaacgac cgccgtaacc tgccagcagc
    19441 agatcgatag cgtaagcgcc catacgggaa gccagaatac ggtcgtaagg caccggagaa
    19501 ccaccgcgct ggatgtggcc cagcacagtt gcgcgggttt cacgaccggt ttctttctcg
    19561 atgaaatgcg ccagttcgtc aacatcacac atatgttcgg taatcgccac gatcgcgtgt
    19621 tttttacctt tcgcgatacc cgctttgatt tcgtttacca ggtcttcacg gctgaattca
    19681 acttccggaa ccacaacgaa ttcacagcca ccggcaatgg ccgcagccag ggtcagatcg
    19741 ccacaataac ggcccatcac ttccaccacg gaaatacgct ggtgagaaga agaggtgtca
    19801 cgcagacggt cgatcgcttc tacaacggtg ctcagcgcag tgaagaaacc gatagtgtag
    19861 tcagtgcctt tgatgtcgtt gtcgatagtg cccggcagac cgatgcacgg gaagcccatt
    19921 tcggtcagac gcattgcacc catgtaggaa ccgtcaccgc cgataaccac cagcgcgtcg
    19981 ataccacgtt ttttcaggtt ttcgatagcc acggcgcgga tgttctcgtc gcggaattcc
    20041 gggaaacgcg cagaaccgag gaacgtaccg ccacggttga tcatgtcaga cacgctgtaa
    20101 cggtctagct gtaccatacg gtcttcatac agacccagat agccgtcata aatacccatt
    20161 acttccagac cttctgtcag cgcagaacga acaaccccgc gaattgcggc gttcatgcct
    20221 ggcgcatcac cgccgcttgt caacacaccg attttcttaa tcatgactac ctctgaactt
    20281 tggaatgcaa aatgaaatct gttgccggaa gtcttcttgc acatcgaagt gatccaacga
    20341 atgtgcaaat agtataacaa tcacttcctg ctgaattgat tcaggtcagg ccaaatggcg
    20401 gtattttata cacaaaatgc gggtctggct ctcttttata ctgattatga aagcatagac
    20461 cgtttaccct ccctgggtac gacggaacag gggtcctgat ggataattac atccgatccc
    20521 ggaaaacgcc gtaaaatagc ctgctctacc tgatccgcca ccatatgtgc ctgaaccaaa
    20581 ggcagagagt cttccatttc caaatgaatc tgaataaagc gggtcggccc tgactgccgc
    20641 gtgcgaagat cgtgagcgcc gctaacaccc ggccaggaag tcacgatatc aataatttct
    20701 tgccgttcct catcaggcaa tgcgcgatcc agtaatgact gtaccgcctc atatcccatg
    20761 cgtaacgcgc tatataaaat atagatgccg attcccaatg caaacagagc atcggcgcga
    20821 tgccagccgt accaggacaa ccccagcgcc agcagaattg cgccgttcat cataacatca
    20881 gactggtaat gtagcatatc agcccgcacc gcctggcttt gcgtccgccg caccacccaa
    20941 cgctgaaacg agacaaggat aatcgtacaa attagcgcca caattgtcac gataaccccg
    21001 acgcctggat ctgtcatcgg tgttggagat atcagatgtt gaatacccgt caaaaacagg
    21061 aatagtgccg aaccggagat aaacatactt tgcgccagcg ccgcgaggga ctcagcttta
    21121 ccgtgaccaa acgagtgatt atcgtcggca ggttgcaggg aatatcgcac caccaataaa
    21181 ttcgtcaacg acgcgccgat atccaccagc gaatccacca gcgcggcgag aatactcacc
    21241 gacccggtat accaccatgc aaaaatttta atcagcaata gcagcgaagc catcgccgtc
    21301 gcagcaatcg ccgcccgact gaccagccgt ccataagatt gattcataaa tactcccgct
    21361 atcaactgac gctagtataa cggaagcaaa tcatctgcaa tgcattaagc agcaggcaaa
    21421 ttgaggataa aaaaaacccc cacatcatgt gggggaagac agggatggtg tctatggcaa
    21481 ggaaaacagg gtttactact gggaacgtga gttgctacta ctcaatagct tcaacgatga
    21541 acttttttgc cattgcgtca cgtcacgcaa ctgctccatt cgttgttgat gtttctcgtt
    21601 taaaaccgct tgctgctccg gcgttaacag gcgatacatt tggttgcgga cttttgccat
    21661 ctcaacctga cgagcaattt gctcattcgc cattttttct gcctgtgcgc gcacagcgtt
    21721 ttcatcaaaa ttttctgcgg tgacaaggcg atgcattgtc tccagttcgc taacattaac
    21781 aggaggctgt tcgtgccggg cctgttgcat aagatctcgc atctgctgac gctgatgttc
    21841 ggttaaactt atgccgtcga acatatggct ctgcgtactg cgctgcgtaa gttcttcacc
    21901 cggatgccag ttatcgcctg aaccgacttc agcagcgtgg cttaatgaac tgactgccag
    21961 cgttgaggcc atgacggcag cggtaactat gcgcatcatt tgctcccaaa atctttctgt
    22021 cgcgattcaa cgatagagag tttacgattc aggctgcaaa catgcgtcag ggggtgtaaa
    22081 acaacgtaaa gtcatggatt agcgacgtct gatgacgtaa tttctgcctc ggaggtattt
    22141 aaacaatgaa taaaatcctg ttagttgatg atgaccgaga gctgacttcc ctattaaagg
    22201 agctgctcga gatggaaggc ttcaacgtga ttgttgccca cgatggggaa caggcgcttg
    22261 atcttctgga cgacagcatt gatttacttt tgcttgacgt aatgatgccg aagaaaaatg
    22321 gtatcgacac attaaaagca cttcgccaga cacaccagac gcctgtcatt atgttgacgg
    22381 cgcgcggcag tgaacttgat cgcgttctcg gccttgagct gggcgcagat gactatctcc
    22441 cgaaaccgtt taatgatcgt gagctggtgg cacgtattcg cgcgatcctg cgccgttcgc
    22501 actggagcga gcaacagcaa aacaacgaca acggttcacc gacactggaa gttgatgcct
    22561 tagtgctgaa tccaggccgt caggaagcca gcttcgacgg gcaaacgctg gagttaaccg
    22621 gcactgagtt taccctgctc tatttgctgg cacagcatct gggtcaggtg gtttcccgtg
    22681 aacatttaag ccaggaagtg ctgggcaaac gcctgacgcc tttcgaccgc gctatcgata
    22741 tgcacatttc caacctgcgt cgtaaactgc cggatcgtaa agatggtcac ccgtggttta
    22801 aaaccttgcg tggtcgcggc tatctgatgg tttctgcttc atgataggca gcttaaccgc
    22861 gcgcatcttc gccatcttct ggctgacgct ggcgctggtg ttgatgttgg ttttgatgtt
    22921 acccaagctc gattcacgcc agatgaccga gcttctggat agcgaacagc gtcaggggct
    22981 gatgattgag cagcatgttg aagcggaact ggcgaacgat ccgcccaacg atttaatgtg
    23041 gtggcgacgt ctgttccggg cgattgataa gtgggcaccg ccaggacagc gtttgttatt
    23101 ggtgaccacc gaaggccgcg tgatcggcgc tgaacgcagc gaaatgcaga tcattcgtaa
    23161 ctttattggt caggccgata acgccgatca tccgcagaag aaaaagtatg gccgcgtgga
    23221 actggtcggt ccgttctccg tgcgtgatgg cgaagataat taccaacttt atctgattcg
    23281 tccggccagc agttctcaat ccgatttcat taacttactg tttgaccgcc cgctattact
    23341 gctgattgtc accatgttgg tcagtacgcc gctgctgttg tggttggcct ggagtctggc
    23401 aaaaccggcg cgtaagctga aaaacgctgc cgatgaagtt gcccagggaa acttacgcca
    23461 gcacccggaa cttgaagcgg ggccacagga attccttgcc gctggtgcca gttttaacca
    23521 gatggtcacc gcgctggagc gcatgatgac ctctcagcag cgtctgcttt ctgatatctc
    23581 tcacgagctg cgcaccccgc tgacgcgtct gcaactgggt acggcgttac tgcgccgtcg
    23641 tagtggtgaa agcaaggaac tggagcgtat tgaaaccgaa gcgcaacgtc tggacagcat
    23701 gatcaacgat ctgttggtga tgtcccgtaa tcagcaaaaa aacgcgctgg ttagcgaaac
    23761 catcaaagcc aaccagttgt ggagtgaagt gctggataac gcggcgttcg aagccgagca
    23821 gatgggcaag tcgttgacgg ttaacttccc gcctgggccg tggccgctgt acggcaatcc
    23881 gaacgccctg gaaagtgcgc tggaaaatat tgttcgtaat gctctgcgtt attcccatac
    23941 gaagattgaa gtgggctttg cggtagataa agacggtatc accattacgg tggacgacga
    24001 tggtcctggc gttagcccgg aagatcgcga acagattttc cgtccgttct atcggaccga
    24061 tgaagcacgc gatcgtgaat ctggcggtac aggattgggg ctggcgattg ttgaaaccgc
    24121 cattcagcag catcgtggct gggtgaaggc agaagacagc ccgctgggcg gtttacggct
    24181 ggtgatttgg ttgccgctgt ataagcggag ttaaactccg catttgtagg caggataagg
    24241 cgtttacgcc gcatccggca tttgagcagg atgcctgatg cgacgctgat agcgtcttat
    24301 caggcctaca ctccgggggt tattttcccc acaaccggcg ggaaaaatct tcgatcttgc
    24361 cgctcagtcg gcgcttttgc atcgtccgcg tccagctttt cgataacccg gcggcggaga
    24421 gtaaacggtg atattgatcg tcatcaaacg gcatatgcca tgcgatggcg gcagcctcct
    24481 gcacggtcac atcgctgaca cgggaaacca actccagcgg cgcgtccgca gacactttcc
    24541 ctggcgctat cacgctatac aaccacccca ccttacctgt gttttgcatc aactgcgcta
    24601 tatcgctgat atcaaaatgg taattgagtt tatagcaagg cgagcgcggc tggctgacct
    24661 gaattaatgc ctctccccag cggaaaatat cgcccatata aacattactt tccgtcaggc
    24721 cgtcggttga gaggttttca ccaaacgcag gcgcaacaaa caactccgcc tgttccggaa
    24781 attcccgcgc ccagtagaga taatgctcac gaggataatg acacagcgct ctgtctggcc
    24841 cgccgtgaac ctttttctcc gcctgctcgt caccttccag ccccagctcc gtcagcatca
    24901 actcaccatc aacctggatt ttggcaattg cgctgggttt gccttcggga taagcctgaa
    24961 tcttgcctgt gtatacatca accggatatc gcatctgttc cctctccacg agaaatttcc
    25021 ccattagacc atatttattt tatgtcaggc gtgctgtttt cgcggtaagt atccaggcag
    25081 gaactcaata gcaaaccgga cgaacgttag ttcatccggc tgtggggatt aaggaagcag
    25141 gtttatttca ccgtacctaa aatttcgatt ttcgctgctc tggctttgac tttacgtgac
    25201 cagatagagg taagaattgg caccagaatc gaagtcacaa tgaccgccgt cgctaccagc
    25261 gaagttgctg ccggagccat cggtttaaac gcaggcacca tttctgcaat cagcacaggt
    25321 gtcgctaccg cggcccctgc ggaactggaa gcggcaattc cggcagtgcc atcgccaccg
    25381 ccaatcaatt tatcggcgat aatcaacgga ataccggtca cgataattac tgccacaccc
    25441 aacaggatcc ccagcaaacc agtctgggca atcacagtca aatcaatggt attgcccagc
    25501 gcgaaggcaa agaatggaat cagcgtttgc accgctttgc tgaaaaattc tcgtaattca
    25561 gggtcaaggt tccccagggc aaagcccacc aggaacggta atactgcgcc gacgaaaaca
    25621 tgcggttcaa acgaggcaat ccctgcagtg cccagaataa tcatcgtcat gagcggcccg
    25681 gactccaacg acatcaacac aaatgcccca gcttcttctt ttgtgccgta ctgctgcatg
    25741 atggaagcgt aaagtccgcc attggtcata tccatcgccg ccaccagcgc cagcgttgaa
    25801 agtccggcaa agaatccaac ttcaacacca tgttccggaa taatgcgcga ggcaattgcc
    25861 gcaaccaccc acgcgacggc aatttttgtt accaccagcg taccggattt acgcaatacc
    25921 gttcccgtcg cgcttaattt tattgacgcc cccatgcaaa aaaaccacac cgccagaatg
    25981 ggcaccgtac cggtaatcat cccgttggtg aatgatccaa aatatttccc cgcccccggc
    26041 gagaaggtgt ggcacagtgc gccaaggaat agcgggacga gcatcatccc ccccgggatt
    26101 ttctcaatcg agcgttttat ctgcatttcc atcacctgtc ggcatatttt taagggctga
    26161 tgcgtcattc ctcaatggag catcagtgtt gatggaaacg aagatagaca ttcatttaat
    26221 caataaaagt gatcaaaacc acatcaattg aaacactgtt ttatttttat cggatcattg
    26281 tttttatttt gaaattgatc acaaaaaaac caccgccgtt ggcgatggtt cattttttaa
    26341 gctgatatgc ggcctatacg cctcattgca gcaggcggca aatgattatt ttttcgccgc
    26401 gaaacgcgcc gcagcttcgt cccagttcac cacgttccag aactctttga tgtagtccgg
    26461 gcggcggttc tggaatttca ggtagtaagc atgttcccac acatccaggc ccagaatcgg
    26521 gaagccggaa gcgccagaaa tagcttcacc catcagcgga gaatcctggt tagcagtaga
    26581 aaccaccgcc agtttatcgc ctttcagcac cagccatgcc cagccggaac caaagcggga
    26641 agctgccgct ttttcaaatt ctgctttgaa gttatcaacg gagccgaagt cacgttcgat
    26701 agccgctttc aggtcaccct gcagggtggt gccttttttc agacctttcc agaacaggct
    26761 gtggttagcg tgaccgccag cgttgttgcg cagtacggtt ttcttgtctg ctggcagctg
    26821 gtccagtttg gtgatcagct cttcaaccgg caggttggca aattctggca ggctttccag
    26881 cgccgcgttg gcgttgttta cgtaggtctg atggtgtttg gtgtggtgga tttccatggt
    26941 ctgcttatcg aagtgcggtt ccagggcatc gtaagcatac ggcagggatg gcagggtata
    27001 gctcatattc atctccagta ttgtcgggcg gccgattgtt aatgccgcgt aagcagttgg
    27061 ttcattatag ttaattaaat gatattgaaa atgattatca atgccgtact tttcgtaagg
    27121 gtatggtttt gcaggaaaat gcccgagatg tgaagcaaat cacccactta atgccgtgat
    27181 tgccagtaaa tcgacaacgg cggcaacagg cgaaaggtta atcgacagca cgatttttac
    27241 actcatctcg tcggagatgt gaagcgacga aaaatgatga ggataagaag atgagtaacg
    27301 cgattacgat ggggatattt tggcatttga tcggcgcggc cagtgcagcc tgtttttacg
    27361 ctccgttcaa aaaagtaaaa aaatggtcat gggaaaccat gtggtcagtc ggtgggattg
    27421 tttcgtggat tattctgccg tgggccatca gcgccctgtt actaccggat ttctgggcgt
    27481 attacagctc gtttagtctc tctacgctgc tgcctgtttt tctgttcggc gctatgtggg
    27541 ggatcggtaa tatcaactat ggcctgacca tgcgttatct cggcctgtcg atgggaattg
    27601 gcatcgccat tggcattacg ttgattgtcg gtacgctgat gacgccaatt atcaacggca
    27661 atttcgatgt gctgattaac accgaaggcg gacgcatgac gttgctcggc gttctggtgg
    27721 cgctgattgg cgtagggatt gtgactcgcg ccgggcagtt gaaagagcgc aaaatgggca
    27781 ttaaagccga agagttcaac ctgaaaaaag ggctggtgct ggcggtgatg tgcggcattt
    27841 tctctgccgg tatgtctttt gcgatgaacg ccgcaaaacc gatgcatgaa gccgctgccg
    27901 cactgggcgt cgatccactg tatgtcgctc tgccaagcta tgtcatcatc atgggcggcg
    27961 gcgcgatcat caacctcggt ttctgcttca ttcgtctggc aaaagtgaag gatttgtcgc
    28021 taaaagccga cttctcgctg gcaaaaccgc tgatcattca caatgtgtta ctctcagcac
    28081 tgggcgggtt gatgtggtat ctgcaattct ttttctatgc ctggggccac gcccgcattc
    28141 cggcgcagta tgactacatc agttggatgc tgcatatgag tttctatgta ttgtgcggcg
    28201 gtatcgtcgg gctggtgctg aaagagtgga acaatgcagg tcgccgtccg gtaacggtgt
    28261 tgagcctcgg ttgtgtggtg attattgtcg ccgccaacat cgtcggcatc ggcatagcga
    28321 attaatcttt ctgcgaattg agatgacgcc actggctggg cgtcatcccg gtttcccggg
    28381 taaacaccac cgaaaaatag ttactatctt caaagccaca ttcggtcgaa atatcactga
    28441 ttaacaggcg gctatgctgg agaagatatt gcgcatgaca cactctgacc tgccgcagat
    28501 attgattgat ggtcattcca gtctgctggc gaaattgctg acgcaaaacg cgctcactgc
    28561 acgatgcctc atcacaaaat ttatccagcg caaagggact tttcaggcta gccgccagcc
    28621 gggtaatcag cttatccagc aacgtttcgc tggatgttgg cggcaacgaa tcactggtgt
    28681 aacgatggcg attcagcaac atcaccaact gcccgaacag caactcagcc atttcgttag
    28741 caaacgacac atgctgacta ctttcatgct caagctgacc gataacctgc cgcgcctgcg
    28801 ccatccccac gctacctaag cgccagtgtg gttgccctgc gctggcgcta aatcccggaa
    28861 tcgctccctg ccagtcaaga ttcagcttca gccgctccgg gcaataaata atattctgca
    28921 aaaccagatc gttaacggaa gcgtaggagt gtttatcgtc agcatgaatg taaaagagat
    28981 cgccacgggt aatgcgataa gggcgatcgt tgagtacatg cagaccatta ccgcgccaga
    29041 caatcaccag ctcacaaaaa tcatgtgtat gttcggcaaa aacatcttgc ggataacggt
    29101 cagccacagc gactgcctgc tggtcgctgg caaaaaaatc atctttgaga agttttaact
    29161 gatgcgccac cgtggctacc tcggccagag aacgaagttg attattcgca atatggcgta
    29221 caaatacgtt gagaagattc gcgttattgc agaaaaccat cccgtccctg acgaatatca
    29281 cgcggtgacc agttaaactc tcggcgaaaa agcgtcgaaa agtggttact gtcgctgaat
    29341 ccacagcgat aggcgatatc agtaacgctg gcctcgctgt ggcgtagtag atgtcgggct
    29401 ttcatcagtc gcaggcggtt caggtaacgc tgaggcgtca gtcccgtttg ctgcttaagc
    29461 tgccgatgta gcgtacgcag cgaaagagaa aattgctccg ccacggcatc ccaattcacc
    29521 tcatcggcaa aatggtcctc cagccaggcc agaagcaagt tgagacgtga tgcgctgttt
    29581 tccaggttct cctgcaaact gcttttacgc agcaagagca gtaattgcat aaacaagatc
    29641 tcgcgactgg cggtcgaggg taaatcattt tccccttcct gctgttccat ctgtgcaacc
    29701 agctgtcgca cctgctgcaa tacgctgtgg ttaacgcgcc agtgggaagg atactgccca
    29761 tccagctctt gtggcagcaa ctgattcagc ccggcgagaa actgaaatcg atccggcgag
    29821 cgatacagca cattagtcag acacagatta tcggtatgtt catacagatg ccgatcatgg
    29881 tcgcgcacaa aacagaccgt gccaccggtg atggtatagg gctgcccatt aaacacatga
    29941 atacccgtgc catgttcgac aatcacaatt tcatgaaaat catgatgatg ttcaggaaaa
    30001 tccgcctgcg ggagccgggg ttctatcgcc acggacgcgt taccagacgg aaaaaaatcc
    30061 acactatgta atacggtcat actggcctcc tgatgtcgtc aacacggcga aatagtaatc
    30121 acgaggtcag gttcttacct taaattttcg acggaaaacc acgtaaaaaa cgtcgatttt
    30181 tcaagataca gcgtgaattt tcaggaaatg cggtgagcat cacatcacca caattcagca
    30241 aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt ttcctgtcag
    30301 taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa ttcagcagga
    30361 tcacattatg acctttcgca attgtgtcgc cgtcgatctc ggcgcatcca gtgggcgcgt
    30421 gatgctggcg cgttacgagc gtgaatgccg cagcctgacg ctgcgcgaaa tccatcgttt
    30481 taacaatggg ctgcatagtc agaacggcta tgtcacctgg gatgtggata gcctggaaag
    30541 tgccattcgc cttggattaa acatggtgtg tgaggaaggg attcgtatcg atagcattgg
    30601 gattgatacc tggggcgtgg actttgtgct gctcgaccaa cagggtcagc gtgtgggcct
    30661 gcccgttgct tatcgcgata gccgcaccaa tggcctaatg gcgcaggcac agcaacaact
    30721 cggcaaacgc gatatttatc aacgtagcgg catccagttt ctgcccttca atacgcttta
    30781 tcagttgcgt gcgctgacgg agcaacaacc tgaacttatt ccacacattg ctcacgctct
    30841 gctgatgccg gattacttca gttatcgcct gaccggcaag atgaactggg agtacaccaa
    30901 tgccaccacc acacaactgg tcaatatcaa tagcgacgac tgggacgagt cgctactggc
    30961 gtggagcggg gccaacaaag cctggtttgg tcgcccgacg catccgggta atgtcattgg
    31021 gcactggatt tgcccgcagg gtaatgagat tccggtggtc gccgttgcca gccatgatac
    31081 cgccagcgcg gttatcgcct cgccgttaaa cggttcacgc gccgcttatc tctcttctgg
    31141 cacctggtca ttgatgggct tcgaaagcca gacgccattt accaatgaca cggcgctggc
    31201 agccaacatc accaatgaag gcggggcgga aggtcgctat cgggtgctga aaaatattat
    31261 gggcttatgg ctgcttcagc gagtgcttca ggagcggcaa atcaacgatc tcccggcgct
    31321 tatcgccgcg acacaggcac ttccggcctg ccgcttcatc atcaatccca atgacgatcg
    31381 ctttattaat cctgacgaga tgtgcagcga aattcaggct gcgtgtcggg aaacggcgca
    31441 accgatcccg ggaagtgatg ctgaactggc gcgctgtatt ttcgacagtc tggcgctgct
    31501 gtatgccgat gtgttgcatg agctggcgca gctgcgtggt gaagatttct cgcaactgca
    31561 tattgtcggc ggtggctgcc agaacgcgct gctcaaccag ttatgtgccg atgcctgcgg
    31621 tattcgggtg atcgccgggc cagttgaagc ctcgacgctc ggcaatatcg gcatccagtt
    31681 aatgacgctg gatgaactca acaatgtgga tgatttccgt caggtcgtca gcaccaccgc
    31741 gaatctgacc acctttatcc ctaatcctga cagtgaaatt gcccactatt tggcgcagat
    31801 tcactctaca cgacagacaa aggagctttg cgcatgacca ctcaactgga acaggcctgg
    31861 gagctggcga aacagcgttt cgcggcggtg gggattgatg tcgaggaggc gctgcgccaa
    31921 cttgatcgtt tacccgtttc aatgcactgc tggcagggcg atgatgtttc cggttttgaa
    31981 aacccggaag gttcgctgac cggtgggatt caagctactg gtaattatcc gggcaaagcg
    32041 cgtaatgcca gtgagctacg taccgatctg gaacaggcta tgcggctgat tccggggccg
    32101 aaacggctta atttacatgc catctatctg gaatcagaca cgccagtctc gcgcgaccag
    32161 atcaaacctg agcacttcaa aaactgggtt gaatgggcga aagccaatca gctcggtctg
    32221 gattttaacc cctcctgttt ttcgcatccg ctaagcgccg atggctttac gctttcccat
    32281 gccgacgaca gcattcgcca gttctggatt gatcactgca aagccagccg tcgcgtttcg
    32341 gcctattttg gcgagcaact cggcacacca tcggtgatga acatctggat cccggatggt
    32401 atgaaagata tcaccgttga ccgtctcgcc ccgcgtcagc gtctgctggc agcactggat
    32461 gaggtgatca gcgagaagct aaaccctgcg caccatatcg acgccgttga gagcaaattg
    32521 tttggcattg gcgcggagag ctacacggtt ggctccaatg agttttacct ggggtatgcc
    32581 accagccgcc agacggcgct gtgcctggac gccgggcact tccacccgac tgaagtgatt
    32641 tccgacaaga tttccgccgc catgctgtat gtgccgcagt tgctgctgca cgtcagtcgt
    32701 ccggttcgct gggacagcga tcacgtagtg ctgctggatg atgaaaccca ggcaattgcc
    32761 agtgagattg tgcgtcacga tctgtttgac cgggtgcata tcggccttga cttcttcgat
    32821 gcctctatca accgcattgc cgcgtgggtc attggtacac gcaatatgaa aaaagccctg
    32881 ctgcgtgcgt tgctggaacc taccgccgag ctgcgcaagt tggaagcggc gggcgattac
    32941 actgcgcgtc tggcactgct ggaagagcag aaatcgttgc cgtggcaggc ggtctgggaa
    33001 atgtattgcc aacgtcacga taccccaacg ggtagcgaat ggctggagag cgtgcgggct
    33061 tatgagaaag cgattttgag ccagcgtggg taaacactgc cggatgcggc acgagcgcct
    33121 tatccggcct acgggtcgaa tgtccatgcg ggctgtcacc ctcaccccgg ccctctccct
    33181 gaaagggcga gggagaaaaa catagcctcg tgcggcaacg gagctgaaca ctgctccgga
    33241 cagtcccctc gcccctacgg ggagagggtt agggtgaagg gaacaagcag caccatgtct
    33301 aaacaaggca acctaattta caggaacaca gaacatgcaa aacattaccc agtcctggtt
    33361 tgtccaggga atgatcaaag ccaccaccga tgcctggctg aaaggctggg acgagcgcaa
    33421 cggcggtaac ctgacgctgc gcctggatga cgccgatatc gcgccttata aagacaactt
    33481 ccatgcacaa ccgcgttata tcccgctcag ccaacccatg cctttactgg caaatacacc
    33541 gtttattgtc accggctccg gcaaattctt ccgtaacgtc cagcttgatc ctgcagctaa
    33601 cttaggcgtc gtaaaagtcg acagcgacgg cgcgggctac catattctct gggggttaac
    33661 caacgaagcc gttccaactt ctgaacttcc ggctcacttt ctctcccact gcgagcgcat
    33721 taaagccacc aacggtaaag atcgggtgat catgcactgc cacgccacca acctgatcgc
    33781 cctcacctat gtactggaaa acgacaccgc ggtctttact cgccaactgt gggaaggcag
    33841 caccgagtgt ctggtggtat tcccggatgg cgttggcatt ctgccgtgga tggtgcccgg
    33901 cacggacgaa atcggccagg cgaccgcaca agagatgcaa aaacattcgc tggtgttgtg
    33961 gcccttccac ggcgtcttcg gcagcgggtc gacgttggat gaaacattcg gtttaatcga
    34021 caccgcagaa aaatcagcac aagtattagt gaaggtttat tcgatgggcg gcatgaaaca
    34081 gaccatcagc cgtgaagagt tgatagcgct cggcaagcgc ttcggcgtta caccactagc
    34141 cagtgcgctg gcgctgtaag gagcaaacat gatccgcaaa gcctttgtca tgcaggttaa
    34201 ccccgacgct cacgaagagt atcagcgtcg gcataacccc atctggccgg aactggaagc
    34261 agtgttgaaa tctcacggtg cgcataacta cgccatttac ctcgacaaag cgcgtaatct
    34321 gctgtttgcc acggtagaga ttgaatctga agagcgctgg aatgcggttg ccagtactga
    34381 tgtttgccaa cgttggtgga aatatatgac cgacgtcatg cccgcgaacc cggataacag
    34441 cccggtgagt agcgaactgc aagaagtgtt ttacctgccg taattgccta atgtgccgga
    34501 gacgcctccg gcacaatatt tttaatttcg atttgaaaaa taaacatatt acaccttcag
    34561 ttaatatatc ccggtaactt atatttcccc gatgcattgt gagtaaatca caaaaataat
    34621 gaataactca ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc
    34681 aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc
    34741 acttaatcgc tccaccatta caattgatta aaatatattt catggagtcc gattaatggc
    34801 agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccattct
    34861 gaagcagttg gcgacaatgg cgttacaaaa cggttttatc accgactcac accagttcct
    34921 gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gcatcgccgt
    34981 gccgcacggt aaaagcgcct gcgttaaaca accgttcgtg ttattcgccc gcaaagcgca
    35041 ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
    35101 gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat
    35161 tcacaaggaa tttattcatc aactgcaaca gggcgatacc gaccaggtgc ttgccttgtt
    35221 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt attgtcgcga
    35281 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
    35341 aagcccgttc tctcggtcat accattaaag ttgaaactca aggatcaagc ggcgtcgaaa
    35401 accgcttatc cagcgaagag attgccgctg ccgattacgt cattctcgct accgggcgtg
    35461 gcctgagcgg tgacgatcgc gcgcggtttg ccgggaagaa ggtttatgag attgccatct
    35521 cccaggcgct gaaaaatatc gatcagattt tcagtgaatt accaacaaac tcgcatcttt
    35581 ttgctgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga
    35641 gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg
    35701 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
    35761 cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc
    35821 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
    35881 ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat
    35941 tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag
    36001 tgcgccttgg caaagcatta caaccgctgc tcggttccat gctgatcccg ttcgttactc
    36061 tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg
    36121 gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc
    36181 tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct
    36241 tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg
    36301 cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc
    36361 gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac
    36421 cggctatacc ttacgttctg gcagctccgt tgccgatgat taccgccaat acgctggcgg
    36481 gtggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg
    36541 gtatctttga cccgctgatc ggcctgatgt cgccggtggg ttcgttttat ctggtgctgg
    36601 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc
    36661 gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg
    36721 tgcgaagcca gcgccgtcag cggcgatgaa caggaagttc gcgacattct gataaacacg
    36781 ctggaacctt gcgttaatga gatcaccttt gatggtctgg gcagctttgt tgcccgtaag
    36841 gggaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc
    36901 acccacatcg acgagagcgg ttttctgcgt ttcaccacca ttggtggctg gtggaatcag
    36961 tcaatgctca accaccgggt aaccatacgc acacacaagg gatttaaaat ccctggtgtg
    37021 attggttccg tcgcacctca tgcgttaacg gaaaagcaaa agcaacagcc gctgtcattt
    37081 gatgagatgt tcattgatat tggcgcgaac agtcgcgatg aggtggaaaa acgcggtgtg
    37141 gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
    37201 gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcaaacg
    37261 gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta
    37321 cgcggggcac aaacctcggc ggaacacatt aaaccggatg tggtgattgt gctggatacc
    37381 gctgtcgcag gtgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
    37441 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtagcagcg
    37501 tttaaaaact gtgccacgca gaatgattta ccgctgcaat tttccaccat gaaaaccgga
    37561 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt cgcgctgtgt
    37621 ctgccaactc gttatctgca cgccaacagc ggtatgattt caaaagccga ttacgatgct
    37681 ctgctcacgc tgatacgggg ttttctgacc accttaactg cggagaaagt caacgcgttt
    37741 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
    37801 tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga
    37861 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcatttccg
    37921 ccagtggtag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
    37981 tgcaaaagca cgataacgac gatcggctgc tggcactgtt attactgaat acctttactc
    38041 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtcgca gagcgtctgc
    38101 cccggttaaa acagcgttat gaacgcgctt gttgcctggc cagccgcccc ggtttgggcc
    38161 atttcattga tgagacagaa gagaagcgcg ttatcttgct ggcgaacttg ctgcgcaaag
    38221 atccgttttt aattccgctg gcgggcgtaa cacgagacaa ccttcagcat ttatccacgg
    38281 cctgcgacaa ccaacaccgc tggccgctca tgcagggtga ttatctctcc agcctgattc
    38341 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaatat cccggtgacg
    38401 agataaaaca aatcgttgaa catagcggtc tgtttcttgg tgataacgct gtaagaaccc
    38461 tgacaggttt gatagagaaa cagcatcagc aagcgcagat aatttctgcc gataatgtgc
    38521 agcggttgct gcaaagggtg ccgggcatcg cgtcattgaa tattattgat acgcagctgg
    38581 ttgagaacat taccgggcat ttattacgtt gccttgccgc accggtgtgg attgctgagc
    38641 accgccaaag cagcatgaat aacctgaaag ccgcctggcc tgcggccttt gatatgagtc
    38701 tgcactttat tacgctactg cgtgaacagc tcgatattcc ccttttcgac agcgatctga
    38761 tcggtttgta ttttgcctgt gcgctggaac ggcatcaaaa cgaacgccag ccgatcattt
    38821 tgctctcgga ccagaacgcg attgccacta ttaatcagct cgccattgag cgcgatgttt
    38881 tacattgtcg ggtgattatt gcccgtagct taagcgaact tgttgccatt cgcgaagaga
    38941 ttgagccgtt attgatcatt aacaacagcc attatttact ggatgacgcg gtaaataatt
    39001 acatcaccgt aaaaaatatc attacggctg ccggtatcga acaaataaaa cattttctgg
    39061 cgacggcatt tattcgccag caaccggagc gttttttctc tgcccccgga agttttcatt
    39121 attcgaatgt acgcggtgaa agctggcaac atattacccg gcaaatttgt gcgcaattag
    39181 tcgcacaaca ccatattacc gccgatgaag cacaacgcat catcgcccgc gaaggcgaag
    39241 gtgaaaacct gattgttaat cgcctcgcca tcccacattg ctggagcgaa caggagcgac
    39301 gttttcgtgg attttttatt accctcgccc aaccagttga ggtgaataac gaagtcatta
    39361 accatgtctt gatcgcctgc gccgccgccg atgcgcgtca tgagctaaaa atatttagct
    39421 atctggcaag catattgtgt cagcatccgg cggagattat tgccgggtta acaggatatg
    39481 aggcatttat ggagttactt cacaaggggt gaactgtgtc ggatgcgccg catccgacac
    39541 tgcatttatc atcaaaaacg tttaacgcag gctattatag tcatcaacca tttcgttgta
    39601 ctcgcgtaat gctctcggga aagagtcttc gaccatccag cttgagtttg cgtcctgaag
    39661 ttgctcttta tcccagtcag agtacgggac tttatctctg atgcggcgaa cgtatttttt
    39721 agcctcgagt tgatactggc ctgccgaatt aataaacgag aaattcatgc cgcctttatc
    39781 cgcttctttg gcctgcgcca ctagcgtttc cagttcagac actttcttca ttgccgcatc
    39841 aacatcaaac ttctcttgcg atatcagatt attaatttgt ttcgctgaaa tcatgactgc
    39901 cagagagtag tagtggaatg ttttcccttc tctttcttca atatttttca gttccgcaag
    39961 ctggcgctta tcgttaattt cctgaatcgc cgcatgataa gattctgcta caggttcaaa
    40021 ggcttccaga ttttttaaga atgtctggtg cagcgttttc cctttcgcaa acgcatcgtc
    40081 tttataattt tcctgggtgt aatatttatc catttcgtta atggtattac ccaacgccac
    40141 ggcagcatcg atataactca ccaccacgcc atcaattggt tgcagcgcag gcgttaacgc
    40201 cacagcactt tttacgcctt tttcacactc agcgaggttg gattcactaa tgccgtagat
    40261 gccataaaca gtacgctctt caccggtagg cccctgttta aaatctttca gccagtcagc
    40321 ataacgcgcc agactgctct gtacagggat ctgtaacttg ttatagcact tgatatagac
    40381 atccattttt tcgtcgaaaa ccgcttgttc atcgaccacc ggttgcgcag gtgtttcagg
    40441 tttagcttct gcttttgcaa cgggagcttc aggagctggt gcggcaggtt gactattggc
    40501 aggcgggggc gtttctgttt cggctttttt atcatcacaa cccgtcagac cgagggccat
    40561 gatggcgact ataatagcgg aagataataa attccttttc atttaattgc cttataaata
    40621 tatagataag agagtgaaca taataaatga aaaaagaaaa cgccactaca cgcgttttat
    40681 ttataaataa aataataaaa ttcacgaaat gaataagtcc tgcaatttaa tatatatcgc
    40741 aggacttatt ttaattaatt gctcaaacgc tgcggatggg tataaaccgt tgcccgaccc
    40801 ggtttacaaa aacctaccag tgtcagatta cagcgctcag ccacttctac agcaagcgtg
    40861 gtcgcggcag acaccgcaaa caaaatctct acgccgcaca tcgccgactt ttgcaccatt
    40921 tcataactgg cacgactgga aaccagtacc gcaccttgct gccagctttc cccttcttgt
    40981 gaccggcggc ctaacagttt gtccagcgcc acatggcgac ccacgtcttc atgcccgccg
    41041 accagttcgc cagatggcaa catccaggca gcggcgtgag tacaaccagt cagttgcccc
    41101 actggctgaa aatcgttgag atgacgtaat gcatcatcca gtttgttgag atcaaacgtc
    41161 tgggtgaacg gtagcggctg caccggtttt ccgatgtcat taagttgctc cacgccgcat
    41221 acgccacagc ccgtacgtcc cgccagcgcc cggcggcgct ccttcaaccc cataaagcgg
    41281 cggctggaaa gctcaatttg tacttcaaga ccattacagg aaggaacgac atccatgccg
    41341 aagatatcgc gcggactttc tattatcccc tcggaaagcg aaaaaccgag cgcaaagtac
    41401 tcaaggtctt tgggcgacgc catcatcacc acatgcgaaa tgccgttgta gaccagcgca
    41461 acgggaactt cttccgcgac ctcatctaac cgtgggtgtt gtaaatcatc acgccgccat
    41521 aactcaattt gacgaacacc tgtgatattt gtcacatttt cgatttcttt ttgctgtgtt
    41581 tttttcactt tctcttaacc agataagaac acacacacca acattatggt attctgttac
    41641 aaacccttcc tggatggagg gaaattgagc caattctgga cctttgcggc cccttccgca
    41701 aagaaaaata actcccactc cctgcacatg cagcaagcga atgtaaatgg gacgtgacaa
    41761 tgtcgaaaca aggagcaatc catgcaggtc agcagaaggc agttctttaa gatctgcgct
    41821 ggcggtatgg caggcaccac ggcagcggca ctgggttttg cacccagcgt agcactcgcg
    41881 gaaacccggc agtataaact gctgcgcacc cgcgaaaccc gtaatacctg cacctattgt
    41941 tccgtaggct gtgggctgtt gatgtacagc ctcggtgacg gagcaaaaaa cgccaaagca
    42001 tctatcttcc atatcgaagg cgatccggat cacccggtca accgcggtgc actttgtccg
    42061 aaaggcgctg gcctggtgga tttcatccac tccgaaagcc gtctgaagtt tccggaatac
    42121 cgtgcgccag gttctgataa atggcagcaa atcagttggg aagaggcgtt tgatcgcatc
    42181 gccaaactga tgaaagaaga ccgcgatgct aactacattg cgcaaaacgc cgaaggcgtg
    42241 actgttaacc gctggctctc caccgggatg ctgtgtgctt ccgcgtcgag taacgaaacc
    42301 ggctatttaa cgcaaaaatt ctcccgcgcg ctgggtatgc tcgcggtcga caaccaggcg
    42361 cgtgtctgac acggaccaac ggtagcaagt cttgctccaa catttggtcg cggtgcgatg
    42421 accaaccact gggtcgacat caagaacgcc aacctcgtcg tggtgatggg cggtaacgcc
    42481 gctgaagctc acccggtcgg gttccgctgg gcgatggaag ccaaaattca caacggcgcg
    42541 aagctgattg tgatcgatcc tcgctttacg cgtacggcgg cggtagctga ctactatgcc
    42601 cctattcgtt ccggtactga cattgctttc ctgtcaggcg tattgctgta cctgctgaac
    42661 aatgaaaaat tcaaccgcga atacaccgaa gcctatacca acgccagcct gatagtccgt
    42721 gaggattacg gctttgaaga tggcctgttc accggctacg acgcggaaaa acgcaagtac
    42781 gataaatcct cctggactta tgaactggac gaaaacggct tcgccaaacg cgataccacg
    42841 ctgcaacatc cgcgctgcgt gtggaacttg ctgaaacagc acgtttcccg ctacacgcca
    42901 gatgtggttg aaaacatctg tggtacgcca aaagacgcgt tcctgaaagt ctgcgaatac
    42961 atcgcagaaa ccagtgctca cgataaaact gcctcgttcc tgtatgccct cggctggacg
    43021 caacactccg ttggtgcgca aaacattcgt acgatggcga tgatccagct gctgctcggc
    43081 aatatgggga tggccggcgg cggcgttaac gccctgcgcg gtcactccaa tattcagggg
    43141 ctgacggacc tggggctact gtcgcagagt ctgccaggtt acatgacgct gccaagcgag
    43201 aagcagaccg atctgcaaac ctaccttacc gccaacacgc caaaaccgct gctggaaggc
    43261 caggtaaact actggggcaa ctacccgaaa ttcttcgtct ctatgatgaa ggccttcttt
    43321 ggtgataaag cgacggcgga aaatagctgg ggctttgact ggttgccgaa gtgggataaa
    43381 ggctacgacg tcctgcaata cttcgagatg atgaaagaag gcaaggtcaa tggctatatc
    43441 tgccagggct ttaaccctgt tgcctcattc ccgaacaaaa acaaagtgat cggttgtctg
    43501 tcgaaactga agttcctcgt caccatcgac ccgctgaaca ctgaaacctc taacttctgg
    43561 cagaaccacg gtgagctgaa cgaagttgac tcgtcgaaga tccagaccga agtgttccgt
    43621 ctgccatcga cctgcttcgc agaagagaac ggttcaatcg ttaactcagg tcgctggttg
    43681 cagtggcact ggaaaggtgc ggacgccccg gggattgcgc tgactgacgg tgagatcctc
    43741 tccggtatct tcctgcgctt gcgcaagatg tatgccgaac agggtggcgc gaacccggat
    43801 caggtgctga acatgacctg gaactacgcc attccgcatg agccgaagtc ggaagaagta
    43861 gcgatggaga gcaacggtaa ggcgctggcc gatattaccg atccggcaac cggtgcggtt
    43921 atcgtcaaga aaggccaaca acttagctcg ttcgcccaat tgcgcgatga cggtacaacc
    43981 tcctgtggct gctggatttt cgccggtagc tggacgccgg aaggcaacca gatggcgcgt
    44041 cgtgataacg ccgatccgtc tggcctcggt aacacgctgg gctgggcatg ggcatggccg
    44101 cttaaccgcc gcattctgta taaccgcgcc tccgcagatc cgcagggtaa cccgtgggat
    44161 ccgaagcgtc agttgctgaa atgggacggc actaagtgga ccggctggga tattccggac
    44221 tacagcgcag cgcctccggg tagcggcgtc gggccgttta tcatgcagca ggaaggcatg
    44281 gggcgtctgt ttgccctcga taagatggcg gaaggtccgt tcccggaaca ctacgagccg
    44341 tttgaaacgc cgctgggaac taacccgctg catccaaacg ttatctcgaa cccggctgca
    44401 cgtatcttta aagacgacgc cgaagcattg ggtaaagccg ataagttccc gtatgtcgga
    44461 accacctatc gtctgaccga gcatttccac tactggacca aacacgcgct gttgaacgcg
    44521 attttgcaac cagagcagtt tgtggaaatc ggggagtcgc tggcgaataa acttggcatt
    44581 gcccagggcg ataccgtgaa agtctcctcc aaccgtggct atatcaaggc caaagcggtg
    44641 gtgaccaaac gtattcgtac actgaaagcg aacggcaaag atatcgatac catcggtatt
    44701 ccgattcact ggggctatga aggcgtagct aaaaaaggct ttattgccaa tacgttaacg
    44761 ccattcgttg gtgatgcgaa cacgcagacg ccggagttta agtccttcct tgtgaatgtg
    44821 gaaaaggtgt aacggagacg acatatggct tatcaatctc aagatatcat tcgtcgttcc
    44881 gcgactaacg gtctgacccc cgcgcctcag gcgcgggact tccaggaaga agtggcgaaa
    44941 ctcatcgacg tcaccacctg tatcggctgt aaagcctgtc aggtggcgtg ttcagagtgg
    45001 aacgacattc gcgataccgt cggcaataac attggggtgt acgacaaccc caatgattta
    45061 agcgccaaat cgtggacggt aatgcgcttc tcggaagtgg agcagaacga caaactggaa
    45121 tggctgatcc gcaaagacgg ctgtatgcac tgttccgatc caggctgcct gaaagcgtgc
    45181 ccggcggaag gtgcaattat tcagtatgcc aacggtatcg tcgacttcca gtccgagcag
    45241 tgcattggct gcggttattg cattgcgggc tgtccgttcg acattccgcg cctcaacccg
    45301 gaagacaacc gcgtctacaa atgtacgctg tgcgttgacc gcgtggtggt tgggcaagaa
    45361 ccagcctgcg tgaagacctg cccaacgggc gcgattcact tcggtacgaa agagtcgatg
    45421 aaaacgctgg cgagcgagcg cgttgctgag ctgaaaaccc gcggttacga caatgcgggt
    45481 ctgtacgatc cggcaggcgt cggtggtaca cacgtcatgt acgtgctgca ccatgctgac
    45541 aagccaaatc tgtatcatgg cttgccggag aacccggaaa tcagcgaaac cgtgaaattc
    45601 tggaaaggca tctggaaacc gctcgcggct gttggctttg cggctacctt tgcggccagt
    45661 atcttccact acgtgggtgt cggtccgaac cgtgcggatg aggaagagaa taatctgcac
    45721 gaagagaaag acgaggagcg caaatgaaac gacgtgacac catcgtgcgc tacaccgcgc
    45781 cggaacgtat caaccactgg atcaccgcct tctgcttcat cctggcggcg gtgagcgggc
    45841 tgggcttttt gttcccgtcc ttcaactggt tgatgcaaat catgggcaca ccgcagctgg
    45901 cgcgaattct gcacccgttt gtcggcgtgg ttatgtttgc ctcgttcatc atcatgtttt
    45961 tccgttactg gcatcacaac ctaatcaatc gggatgatat cttttgggcg aagaatattc
    46021 gtaagatcgt cgtcaacgag gaagtaggtg acaccgggcg ttataacttc ggtcagaaat
    46081 gcgttttctg ggcggcgatt attttcctgg ttctgctgct ggtgagcggc gtgattatct
    46141 ggcgtcctta ttttgcgcct gctttctcaa tcccggtgat ccgattcgcg ttaatgctgc
    46201 attcatttgc cgcagtggcg ttaattgtgg ttatcatggt gcatatctac gccgcccttt
    46261 gggtgaaagg cacgattacc gccatggtgg aaggatgggt taccagcgcc tgggcgaaga
    46321 aacatcaccc gcgctggtac cgtgaggtcc gcaagacaac ggaaaagaaa gctgaatgag
    46381 tattcgcata atcccgcaag atgagctggg ttcgagcgag aaacgtacgg cggatatgat
    46441 tccgccgtta ttgttccctc ggctcaagaa tttatacaac cgccgcgccg agcgtctgcg
    46501 cgagctggca gaaaataatc cgctgggtga ttacctgcgc tttgctgcgc ttatcgccca
    46561 cgcccaggaa gtggtgctgt acgaccatcc gctggagatg gatctgaccg cgcgcattaa
    46621 agaagccagc gcacagggca agcccccgct ggatattcac gttctgccgc gtgataagca
    46681 ctggcaaaag ctgctgatgg cgctgattgc tgagctgaaa cctgaaatga gcggcccggc
    46741 gctggcagtg attgagaatc tggagaaggc atcaactcag gagctggaag atatggccag
    46801 cgcactgttt gcctctgatt tctcatccgt cagcagcgat aaagcgccgt ttatctgggc
    46861 tgcactgtcg ctctactggg cgcagatggc caatctgatc cccggcaaag cccgcgctga
    46921 atacggcgag caacgtcaat attgcccggt ttgtggttct atgccggtgt ccagcatggt
    46981 gcaaattggc accactcagg gtctgcgtta cctgcactgc aacctgtgtg aaaccgaatg
    47041 gcacgtagtg cgcgtaaaat gcagcaactg cgagcagagc ggcaaactgc attactggtc
    47101 gctggatgac gaacaggccg cgattaaagc cgaaagctgc gatgactgtg gcacttacct
    47161 gaagattctt tatcaggaaa aagaaccgaa agttgaagcc gtggcagatg acctcgcttc
    47221 tctggtactg gacgcgcgaa tggagcaaga aggctatgcc cgcagttcca tcaacccgtt
    47281 cctgtttccg ggtgaagggg agtaatctct gacgttttcc gggtggcgct ggtcgcccgg
    47341 attacagctc atagggtgtg acgacaccat ctctcgtatt ccgcgttacc cattcaagat
    47401 catgccgcaa ttgtctgttt gcctcgttct tccctccaac acatcgataa ggttatgggt
    47461 atgtcactga tgacaagcgt atgattgcct gatgcgctgc gcttatcagc cctaccaata
    47521 aacttcaata tattgaattt gcatgacttt gtaggccgga taaggcgttc acgccgcatc
    47581 cggcatgaac aacgcgcacg ttgtcagcaa tctgattgac atcatttttt cccattacca
    47641 ttcctcgcgc agcttacgct gatattcgac accatcttcc tcacaccctt gccagatgcc
    47701 aggaacactg gttcttgctg tttgcgattg atttatcagg tattgatcta ccgcttccct
    47761 taataggtcc gcacgaggaa gattacgctg cacctcgaga tcatcaagtt gcttaatcac
    47821 ctcattcgat aaatcgagta aaattctgcc catatctata cctaccagag agttcataaa
    47881 tatctcgcca atatcatttt tgaatctatg gagagaaaag tacccttgtc gaatctttaa
    47941 agaaagctca tttacgaatc attttttatt tttgcgaggc gttttccaga tatgatgtaa
    48001 cagttgcagt tattataagt tcaagtaaaa caaccttgta ttaataccag aataagggca
    48061 tctggttttt tatattcaag aataaaaaaa tttttgtcat tccttatgat ccttacactg
    48121 atcataaaca cctactgtct cacccctttg cctgcctctc tttgctttac gcaccggaaa
    48181 ttgctgccgc tccagatatt gctcaaccgc ttcgcgtaaa attgcagatc gtgaaacctt
    48241 acgccagccc cgaagcttgt tcagccgttt aattgcctct tctgataaat gaagaaaaac
    48301 cgtattcatc gccattacca ccaccttaca tatattactc attaatgtat gtgcgaaaat
    48361 tacgccggaa aatcatttaa aaaaagcgga tttttgcatg ttgttttgtt tttgcgaggt
    48421 gttttccaga aataataaga gtgttgcagt tgtttcattt attactcaaa aatggtttag
    48481 ttatcgccca ggatgagtta tggatgctat gaatatatta tattcagaga tatttatttg
    48541 cataatctga atggtgtagc cgaaaaacgt cagcatccta acagcaccag gatttaaggt
    48601 gaaattaatc atccatcaca gaccggtcag tactgccacc aaccggtctt tacttgttca
    48661 tcgttataat tttgttggta acagttcggg aaacaaggaa ttccttccgg aaccccccgg
    48721 tatcatccga tagcattgac gtcaatcatc agcgcgacga tgagtttttt taatcgcatc
    48781 atagggtggc gacgttcagc acgattacgg gtcatagcaa aactcctggg caggcgacca
    48841 ctatgccgct ttcacaggag ttctgaatgc tagtgctttc tgtatggcat aaccatgcct
    48901 ctaacggtta agtgcttgca gcacatgttg cggatcattg gcaatcgctc gcagcaaagc
    48961 ctttgcaggc ccggtcggtg aacgtcgccc ctgctcccag ttacgcaacg tgtctacgct
    49021 tacagaaata agatcggcaa atttagattg tgacaaccct gaagcgcggc gtatttcttt
    49081 gattttcata gcatcaactt caaaagtccg cgaaggctgt cgcgttcctt caataatttc
    49141 attcatctgg gccatgctgt gagtcaaccg ctcaaaaagt gccttatcca tttaccacct
    49201 cgtatttaac aaccggagca ttgctttttc ctggggagac agatcatctt taataccctt
    49261 acgataaata agcagtaatc tgatctcata atgtttaacc tgatgaaagt aaataaccct
    49321 gacaccagca cgtttcccct ttcctcctga aacccatcga acctttctta acccaccagt
    49381 ttcaggtatt acctcaccat agtcaggatt caaggccagg aaaaactgaa aacgactgaa
    49441 ttcatcgtcg gtcagcagtt tttgaacatc ttcagtaaaa atttccgttt cgatgaagag
    49501 cataattata cgccattggc ttagagtaac aatccttttt gtacgtcatt gacgtaccct
    49561 tgaaaccact agcttactga tagtgctcca gcaaaaccac gtctctgccg ggtttgtctt
    49621 tagatttgcg aggcgctttc cagatttttc acgcccgtga atttccctgt atccatccag
    49681 caaaaacggg tgtataagac tgtacattca tacagcaaaa taagttctac tatggcactg
    49741 gaaaaaggaa ttgcagagtt agtcgaaaag tttatcgctg cgggtcgccc ctcatcgcgt
    49801 caacaaagta ttgcgcagcg cagggagggc tatatcgcca gcgctgtcct tgcgggtgag
    49861 acagaaacgc gggtagatat tcagactatc gaacttgaag ggatgacctt acgaatagtg
    49921 tcgccgctga atgccccgac attactcccg acgataattt attactacgg tggctgtttt
    49981 gttagcggcg ggttcgcaac tcacgacaac cagctgcgcc agttagccta ttacgggcaa
    50041 tgtcgggtta ttgccgtcca gtatcggctg gcaccggaac atactttccc cgccgcccat
    50101 gatgatgcgc aacgaggtgc agaaatagtc tggcaacacg ccgaacgact cggcgttgat
    50161 aaacaacgta taactcttgc cggtgacagt gcgggcggac atctggcgct ggtaactgct
    50221 ttgcgattaa agcgtgctgg cgagtggcaa ccggcacaac tcatcttaat ttatcctatg
    50281 cttgatgcga ctgcgcattt cgaaagctac atacgcaatg gtcacgatta catcatcaca
    50341 cgcgacactt tactgagcgg ctttgagatg tatctgccag gcattgaacg caggcatcct
    50401 gaagccagcc ctctttggcg cgatgatttc aatggcttgc cacctgtaca tatcattacc
    50461 gctgaatacg acccactttg cgatgaaggt gaagcgttgt atcaccgcat gacagggcaa
    50521 ggagtgcaat gcacctgcca acgctacctt ggcgttatcc acggattttt tcagttaggt
    50581 ggtatcagcg aggccgcgcg atcggcacta cgggatgttg cctggagagc gggaaggtaa
    50641 cttcggacct gggccctgaa aaggttttca gattgaaaac cttttcagtc aagtaaaacg
    50701 cccctactct tcttcgttcc cgccctcttc atacgggcca aatggcttcg cgggcagaac
    50761 gatatacgtg ccttcaaaca ctgcacccgg cgtctcgtcg ccaaagattt caacttgcat
    50821 ctgcacccgt gcttttcgtc cgcgcgccag acggtcgaga tcgccgctta aggcaccaag
    50881 gtcggctacc gcatgaggtt taccgctaat cggcttgctg tagcggatat gcgcatccgc
    50941 cagaataatc gttccgccga gatggcgttc gcgcagcatc agccagataa gcccccaacc
    51001 ggtgagcgtc gccagcgaga ataaactccc ggcaaacagc gtatggtgcg gattctgatt
    51061 gccggtttcc ggcatggtgg tgataaattt ttgcccggta tattgttgaa tacgcacgcc
    51121 cattttttca ctaagcggga tatgttcgta ccacgcctgt tgcagctgcg cgcaccagtc
    51181 gccgcgatgc aaaatatcat ccagagtggc gacgggctta atcatcaaaa aatggcgaat
    51241 cggcgtggtg gttggcgtgg tgatttctcc ctgattaaca aaccccagct tggcgaaaaa
    51301 ctccaccgcg tcttcacggg cgctacaggt cacgcgctta acgccttcct gacgcgccac
    51361 cgactccagg gtcatcgcca tcagcgttcc taaccctttg tcctgcacgt ccgggtgaac
    51421 ggccataaag cgaatggacg cttcattgtc ggcattaata tacagtcggc ctaccgccac
    51481 cagattaccc tgctcgtcga cgaccatctg gtgatgcgcc atcgcatccc acgcgtcgcg
    51541 ttccgaacct tttggttgat gcaggggctt acgcaacatt tcccagcgaa actgatagta
    51601 acgctctaat tcttcttctg tttgtggaac ccgaaggtga tacatagcga tactctctct
    51661 tgtttcccgt gacaaccctg gaagctggct catacctgca accagaatgt cacggggcca
    51721 tcattgacca gcgatacctg catatcggca gcgaagcgtc ctgtttgcgt gttcatctct
    51781 tgctggcggc agcgttcgac gaaatagtca tataacgcct ctgcgcgatc cggtgatgcg
    51841 cctttggaga aacttgggcg catccctcgt tcggtatctg cggcgagggt aaactgggaa
    51901 accaccagta cactgccgcc cgcctgttgc acgttgagat tcatcttgcc ttcggcatcg
    51961 ctaaagatgc ggtagccgag cacacgctcg cacagacggt ttgctttctg ttcgtcgtca
    52021 tccttttcga cacccaataa caccaaaagt cccgcgccaa tttcgcccgt cacttctccc
    52081 tccacggtga cgctggcacg ggttacgcgt tgaattaatg caatcatggt tcgtcgtctt
    52141 cttcttgttc agctgcttgt tttagtttgc ggtattcccc gagagtgaca gtaatttccg
    52201 cgccaagcaa gacgatacac catgtccagt agacccaaac aaagagaatg gggatcaccg
    52261 ccagcacgcc gtaaatgagc tgatatgacg ggaacatggt gatataaagc gcgaaacctt
    52321 tctttcctgc ttcgaacagg agtgcagcga caaacgcgcc gacaatcgcg tcgcggttag
    52381 gtacgcggat ggtaggaaca atgctgtaca gcaaccagaa ggagatccac gacaacagca
    52441 gcggaaaaat acgcagcacg ttgtcgatga cagtattgag atcgctcgcc cagcgcaggg
    52501 agagcaaata ggaactgatc gccagactgg cccctgccag cagcggcccc agcgttaaaa
    52561 tcatccagta cacggcaaac gagtaaattt ttggtcgcgc tcgtttactg cgccagatgg
    52621 tattcaacgc gctatcgatg gagtacatca acaataatgc ggtgacgatc agcccgcacg
    52681 cgccaacggc ggtcatcttg ttggaattgg caacaaattg ttcgatatac cgctgaataa
    52741 catcgccagt agcaggcaga aagttggcaa aaataaagtg acgcaactga atgctgacgt
    52801 cggaaaacat gggaaaagcg gcgaaaagcg caaaaacaac ggcaaccagc ggcactaatg
    52861 agagcaaaga cacataggca aggttacctg ccagggttgt catgttgtcc tcatcaatac
    52921 gttgccagag tagttttagc caggcccata gtggacgagt acggtgcctg gctttgtcct
    52981 gaatggtttt tagcataaca ccttcgcgaa atagtccggg atggtggttt tatctttcac
    53041 cagaatactg gtaatgccca gctgattggc tccttctata ttatcggcgt tatcgtcgaa
    53101 aaagaccgta tcgctgggtg aaaaaccttc cgcctgcaaa acatgctggt aaattcgtgc
    53161 ttcaggtttg cgcatcccca gatcttgcga cagatagata tggtcagcag catcacgaat
    53221 ttccgggtat tcttccggcc agaaggtggt atgcaggcgg ttggtattgg aaagcaccac
    53281 cacgcgatgc ccctgctcac gcagtttatg catgatggcg atcacttccg ggcgcagcgc
    53341 aacaaacacc gcctgccagc cgtgagagaa ctgctcgtag cttagcggta gagccatctc
    53401 atgacacagc gcctctgcga acgcttcgtc gctaatttcc ccacgctcat gctgatgaaa
    53461 cgcctccccc atatgaaaac tcttcttaag cgatgccagc ggaatacgcg ttaaatcgct
    53521 ccaggctccc agcacacggt taaagtcgat atcgacaatc acattaccta aatcaaagat
    53581 atagagcatt tttgcctcct ttcgcgccac aagaaattaa ctgtagcggg aaaggatggg
    53641 tttggctatg tggcaataag ggtaatcttg tgatgctgat tgcgaaaaag cagagtaatt
    53701 tcactggctt tttcgcaata gatgaatggt taagattcag gtgtaaattg ccaacgagtt
    53761 tcgatgacca gcggcctatc tggtgtcacc aattccggcg ttagatttat tgcgttaggt
    53821 gcctgagtca gcgggttaat acaggtggca tcgggctgtt tatcaaatac caccagactg
    53881 tttgcagaag agatcatggt catcgcgagt ttaccaggcc acagtaactt aaccttcacc
    53941 ccgtcataaa aaccaaaaca gtcatcccag ggaccggctt gaggagagat acgatttcca
    54001 gttggcagtt catcactacc cgcttcttct tgccagtccg catcaaaaag cacctgcaac
    54061 gattctgtat tttgcggagt cagctttttc gcaaaccacg gatgccagcc tgcggaggcc
    54121 gggaatgtat cggcatatga atggacttcc agttgcaaaa ccagcgcatc gttctccagc
    54181 aaaaaagtct gaatcacttc cccttgccag ggccacggtg aagccagggg catacgtaaa
    54241 gtcagggaat ctgctgtttt gtcgatgatt tcccacgttg agtaacaagc cataccatgc
    54301 aacgcatgtg gtggtttatt ggcgggtaaa gaataacatt gcccaccagc attcagcgtc
    54361 gcattcccca gtcgccctgc ccacggcacc attgggaaac agccatactg aaaagctctg
    54421 cgctgcggct gccattggcg taatacttca gaaccaaatg cctttaacga ggttatcctg
    54481 gcaccatcat gcggataaat ctgcaggtga atattgcctt tttccagcgt cactaactct
    54541 cctacaggcc caggccatgg cgcgtcaaac gcctgttgta gcgccaggta atgctcgttt
    54601 tttcgttcag ttttcatgag attgttccct gatgtagcgg taagaagcgg tttcttgttc
    54661 atcggtcggc tcaatcagcc agacgcaaac aaaggaaaga actgagacag caaataaaaa
    54721 cagagcgaca taccacggtt ttcctccccc tagcgccagc aaacttgagg cgataagggg
    54781 gcttagtccg ccaagaatcg cggaaaattg ataagcaaaa gaaaggccgg tatatcgtac
    54841 tttggtaccg aacatgcggg tgaaaagtgt tggctgcacg gcaaacatca tcgtcggacc
    54901 taagttgtag ccgaggatca tactgcacca gatgatgagc gtacttttgc tatccagtaa
    54961 taagaagaaa ggaaacgcga acaggacaca aaatcctgct cccgacaaat acagactttt
    55021 ttgacctata cgatctgata accatccaac caatggacaa ctgaaaatac cgatcgccga
    55081 ggcaatcaac atacccgtca ggggaatatc tcgcgacaat gctaattggc tggaaatata
    55141 gacaatacca aaggcgttaa taatattaga ggagacactt tccgccagcc tcgcacccag
    55201 tgccaataaa atatttcgtg gatgtttttt gaataattca atcaacggag gaacggactt
    55261 ttctttatta ttcgtcgttt tttgcttttg aaaatccagc gtttcttcgg tatgcaggcg
    55321 gataaacagc ccaacgatca acatcaccgc agaaagccaa aacggaatgc gccatcccca
    55381 ggagagaaac tgctccgaag taagaaaatg gttacagaga gcaaaaatac cggttgccag
    55441 cataataccc acagacgcag ccgtttgcgg gagtgaacct aaaaatccgc gtcgggattc
    55501 gggcgctgat tctatggtca tcaatgccgc tccgccatac tcgccaccaa gaccaaagcc
    55561 ctgaattaaa cgcagcgcca ttaatatgag cggtgcccag atcccaattt cctgatacgt
    55621 cggaataaaa ccaatcaaaa atgtggagca accgacaata gccagtgtcc agataagtgt
    55681 tatttttcgc ccaattttat cgccaaaatg gccaaagacg ataccaccta aaggacgggt
    55741 caaaaaacca acgccaaagg tagcaaaggc ggctataaga cctataaaag gatcgctgtc
    55801 tggataaaac agcggtgcaa ataccagtcc tgctgccgtg ccaaaaataa agaagtcata
    55861 ccattccatt aacgcgcctg cgaaactgcc cataacgaca cgtctcatgg ctttaccgga
    55921 aagcccttca cgcggtgcgg gtaacttttc agacataatt ttcctctcct ggtaggcgag
    55981 aaatctggga tacgaaccac taaacgtggg cagtttgaat taatgcgcat cggtttaagc
    56041 cgatgcgcat taatttattt cttcaattca gccagaacct ctgcatcctc agcatcaaca
    56101 tttgcaccca gtggttccca attaatcaat acgcaggtca ggaaccccaa gaatggcacg
    56161 attgccaacc agtaaaacgc ttcagttttc aacgccccca cagcatcggc cagaaaatga
    56221 gtcctgcaat tgcccctgtg cgcataatag cgcgggcaaa tccgacccca gtagggcgaa
    56281 tagcgggagg ataagagagt gtggcaatgg tcatgcccag cccaccagga cccgctgaat
    56341 ggaagaaaat aattgatccc aataacccta aagacagcca tggattagtc gttgccagtg
    56401 cgcctaacga cagcaatgct aaggtcacaa cagcaaatcc atacatcgac tgtcggcgag
    56461 tacccagtcg ttggagaata aacgagccga tcatcccgcc tgtcacccca caaagggcat
    56521 tcacgacagc agaacccgtc aacgcaccag taagcccgcc acttattatt cccgccaacg
    56581 taagaggaag ataaacacct acggcgttat attgccacgc ctgcatggtt gccacgacac
    56641 agccaagaat ggtgcgtttg cggtaacgat cattaaataa gatgccatat ccaccactgt
    56701 atttattctc tgctttttcc gagaatttcg cttctgttgt actcgatgcc gcaacgcgag
    56761 cctgaacacc ataacgtttt cccagaatgt cgcacgcttc ctggtaacgc ccgacgcgtg
    56821 cggcccacat tgcggattca ccaataaaga agtaacgcaa aatcatgaag atgactgcaa
    56881 aaatagctcc gatgaatatt ccgtaacgcc acaattgttc ttcagcgata cccgacagaa
    56941 gcagcggcaa gagcaccaaa taaacaacag ttgtcgaaac ataccaggcc atttgccaga
    57001 ggttgaccga cgttccggtt ttcttcgacg ttgaaccacg aagctctgcc acggcattgg
    57061 ttgccaaagg gaagtcgatg cctaaaccaa agcccatgat gacgcggcac accagcaccc
    57121 accagatact gggcgcaata gctaccgcag cagccccgat ggtgcacagc cccatgccaa
    57181 taataaatgc tcgcttttgc ccaaagcggt ttattactcg gttcgctaat aaaccgccaa
    57241 ttaacgcggc gattaatact gacgcattaa cggttgcggc aaggcctgga gaaatgccaa
    57301 attgagcggc aatgtattta ttaccaaacc ccaccatcgc cgcctggtag gcatcaatca
    57361 atatgccgcc caaagcaata aaaataacgc cgatactggt gttgattgca gagttcttat
    57421 taacaatatc aattacgtcc tgtggccgac taattgaata agttgcagga ctggtgattt
    57481 gactcatcgc agtgtccttt ttaattgttg tagggtggcg ctgacgtgac gcagcgccag
    57541 acagagattt agaaaatcat tccggcgcaa attgcagcat gacctttatg gagtcatttt
    57601 gctgcgcgcg ttcgaatgcc gccgctgcat cctcaatggc aaataaatcg gtaaccagtt
    57661 cactggtatc aaaccgccca tccgccagcc attcgataac agcatcgaaa tcttcaggga
    57721 tgtacatgcc agagttgagc aagtcacgct cgaaacgttg catcatcggt aatggtattt
    57781 cacgcggccc acttggtacg cccatgcaca cgacggtgcc tccggcatat acacgggtac
    57841 atgcatcgtt aagcgtggcc tgcgctgcaa cgacatcaat cgcccccgta aatcgcacat
    57901 ctgcggccag ctcacctggt gcccaaactt ctgctgcgcc cagctttaac gccagcgcgc
    57961 gtttggcagc gtcaggttca atcacggtaa tcttacctgc ccccataatg cgcagtgcct
    58021 gcacgatgga taagcctatc gttccggcac caatcaccag tacgctttcc agagaagctt
    58081 tcggcattcg gttaacgcag tgacgggcac aagccgccgg ttcggcaaag gccaacactt
    58141 ttagcggtaa agaggctggt gcgacatggc aattacgcgc aggaacaatg tgttgtgagc
    58201 gagcaaagcc cggtgcgcga aaaccagcaa cctgcggtgg ttcacaaaga ttaaaacgtc
    58261 ctgctttgca ggctcggcat tccatgcaag ccatgatggg atcgaccaca acatgatcgc
    58321 ccggctgtac atttttaacg tcgctgccaa cttccgtaac gcgcgctgca atttcatgac
    58381 cggggacgac aggtggttta gcaaacggat gaccgccttt cagaacatgc agatccgaac
    58441 cgcatatccc atagaacaca ggcgcaatcc gcacttcgtg cggtttaagt tcctggtgtg
    58501 gtacttcaac atattgagtg gtgactttcc caatatctga aaataaaacc tgggtaattt
    58561 tatccattgt tattctccag aatcgatatc cataggggat tagcgttgcg cgataccttc
    58621 atcgacaatt tctgccaacc gttgtaaacg aggaacagag acatcgcgca gcatagtttg
    58681 tggatcttgt gcaccaacaa ccgacgccca aaccgctcgt ccagccagga agcctgacgc
    58741 accacctttc atggcaatac ttactgcacg gccaaaaata tcggcatcaa cgccagaaga
    58801 aagaattacc cacggcatct tcatctgatc gttaagttgc tgacaggcgg caagtaaggt
    58861 tttttcatcg cctttgcccc ctaatggcat ctcggctttg taaagatcag cctctgttcc
    58921 accaagctct gcggcggcgg caacaatggc gctttcacga tcaaagtccc aaccgcgacg
    58981 aggtggacgc accacaggtt caataatgct aaccagcccg gcactacggc agcgacgaac
    59041 aaatttatca accattgcca gacgttcctc tgcgggttca tcttcacgcc agagtacaag
    59101 taatttcatt gccttagccc ccatttcacg ggctttatgc gggtcgacgg acatatctat
    59161 ttcaacgcta tcaaccggga tgccattacc tgggataaaa agatcggcag cgacaatcag
    59221 gccgcaagaa ttagcaacag cgccctgctc gactatttgc tcaagacaaa attgtttatc
    59281 cgccagaacg gcagaagcat aaggagaaag gattcgggtc gcggcaactt taaaatcggt
    59341 gagtacactg tcggcgatgg gttttggctg ccctgctgcc gcaaacatca agcgcatggc
    59401 ctcacgctga tcgacagcta acatggcaaa tccaccacct gggcgggaga tatctttcag
    59461 cgtaaatggg gtgaagtggg atgacataga aactcctgtt aatagataaa attaaagacc
    59521 agcctgctgg cgaacctgat ttagcagggc actccagtcc tgccgccctt tgccgtcttg
    59581 acttgctgca gcgtaataac tttctgctgc aatccccata ttgagcggtg cccccgtctc
    59641 ccgagcgacg tcaactgcaa tacgcagatc tttcaacgcc agatccacca tgaaggcggg
    59701 tgaaagatcg cctttcagca ctttgcaagg ccaggtggtc gtaaaatgtc ctttccctgc
    59761 tgccgtacca ctcatcacct taattgcgac atcaagattt aatcccagtg actcacataa
    59821 cacagcggcc tcggaggaca gcgcattaag cgcaatgctc atatagttgt taattagctt
    59881 cacgcggatc cccattcctg ggccgccagc ctcgaccagc tcattaccca tgcacatgag
    59941 aatcgggcgt gcacgtgcaa tctgctcatc agtgccaccc gctaaaatca gcaatgtgcc
    60001 atcaatcgca ttcacggacg ttctgcccac gggggcgtcc atcatactga tgcctttttc
    60061 ttgtaactcc cgtattaatg catcggtctg taagggatga atagtcgaca tatcaatcaa
    60121 taacgcgtca gatgaaacag cctcacagac gcctttttcc cccaacagaa cctgtcgaac
    60181 aatgtcacca ttgggaagca tagtgatgat aaattcagcg cctgctgccg cttccgctgg
    60241 ggtttgtgcc gcagtggccc cctgcgtaac tagcgcatcg acggcctgtg cattgacatc
    60301 aaatacctgc aatgaatgcc ctttttgcaa tagattgtta gccattgggc tacccatctg
    60361 ccccagcccg agaaatgcaa tggttgccat gaatactctc ctgtctgaca ttgacaaaat
    60421 aatgacaatt gaaaattcgt tttcgtctgt ttttgagcat atttgtaatt tatgttcaaa
    60481 aacacgacac gcatcactaa tttgatcatt tgtgtcattt aaattgaccg aagacgacaa
    60541 aattaaacag gacaagacaa atatgaccaa aattgcatgc gtgggtatca cagttcagga
    60601 caggatttat tctttgctca ctctgccgga tggcggcgga aaatatcagg aaaatcatta
    60661 tcttgagatt ggtggcggcc cggcagcaac agccgctgtt gcaatcgcaa agcttggcgt
    60721 agaggttgat ttcatcggtc gcgtcggcga tgacagttgt ggaaatacct tacttgcaga
    60781 acttgagggt tggggcgtca ataccgcatt ttgccgccga tatcctaacg cccgttcatc
    60841 gcaatcagcg atcctcgttg atcagcatgg cgaacggatt atcgttaatt atccaagtcc
    60901 cgatctcggt actgatgccg aatggttaga agcgatcgat ttctctcgtt atgatctgat
    60961 tctggccgat gttcgctggc atagcggtac tgaaaaagca ttctcgctag cgcgtcttgc
    61021 gggtgtgaca acgcttttag acgccgatat gacaccgcag gatatttctc cacttgtcgc
    61081 gcttgccgat cacgccgtgt tctcaacacc agggctgaaa cgcatgactg gcctgcagtc
    61141 acctgaagaa ggtttgttcc aggcaacgac acaaacagca ggtaaagtgt atgttacgct
    61201 gggttctgaa ggatcgttgt ggattgaaga tggtcatttg tgtcaacagg aggcattttc
    61261 tgtcaatgtg gttgatacca ctggagcagg cgatgttttt cacggcgcac tcgcagtagc
    61321 gttggcagaa aaaatgccaa caaaagaggc tattcgtttt gccagtgcgg ttgccgcgat
    61381 gaaatgtacc cagcctggcg gtcgggccgg cattcctaat cgtgagcaaa ccgaatcatt
    61441 tttgtcactt tatgcgtaaa atggtaaaca tctaccgagg aggggcaagg gagtattcat
    61501 gagtatcatc gaagtgacag gtaatccacg ccatgaccaa ctcgttcatc taatcgcaga
    61561 acgcggttat atgaatattg aagaactggc acaactgctg gatgtttcaa cgcaaaccgt
    61621 gcgccgggat atccgaaaac tcagtgagca gggcttgatc acccgtcatc atggaggggc
    61681 cgggcgcgtc tccagcgtca tgaataccgc ttttgagcaa cgggaacttt cgctcaccgc
    61741 cgagaaacgg gcgatcgctg aggcagtcgc cgattacctt cccgaacgct gtaccgtctt
    61801 tatcaccatc ggtacaaccg tagaagccgt tgccagggca ttactcaacc ggcgtgattt
    61861 acgcattatc actaacagcc tacgtgtggc acagattctt tataagaatc aggatattga
    61921 agtgatggtg ccgggaggca ctttacgcgc tcataacggc gggattatcg gcccaggagc
    61981 cgtggatttt attgaaggct tccgcgcaga ttatttaatc accagtatag gtgccattga
    62041 acatgacggc accctactgg aatttgatgt aaatgaagcg ttagtcgcga gaacgatgat
    62101 taaacatgcg cggaatacat tgttagtggc cgatcatacg aagtttgccg cgtctgctgc
    62161 cgtttcaatt ggcaatgcac ggaatgtcag ggcttttttt acagatgccc cgcctcccaa
    62221 ttctttctgc cagttgttaa gtgaagagaa tgttgaactg gtggttgccg agcaggaagt
    62281 ttcctgaaat tgaaacttcg aaaaatccgc tggggattca gcggatttta ttttgccgga
    62341 tgcggcctac ggttgatacg gttttcgttg gccggattaa gcacatccgg ccattaatta
    62401 agcttcagcc gccagcatct ctttcttacg ttggctaata cgcttaacca gcattacgga
    62461 aaccaccgca ccacagaatg cgaagcaggc cataatgcca aacacgattt tgtagccaat
    62521 aatccccgga tttaaatcca gaatgtagcc atacagactg aagcagaaca tcgccggagc
    62581 gtaaccaata aagctaccca acgccatcgc cgcgcctgtt ttattttcag caatttttgc
    62641 ttcgccgata ggtgcaaaaa atacggctcg ctgtgtaaag actatcgcgc caaagcccag
    62701 cgtacatgcc atccctaaat agaccggcat actttcgtgc ggcagcataa tcaacagtac
    62761 gagcgcagcg gtactgatga taaaggtgta gcatagatat ttactcggcg atttcaggat
    62821 cttatctgaa atcatgccac caatcggtcc accaatcatt ttcagacagt attggttaat
    62881 gatgccgtaa gcccccacca gcgcaacggg caatagatag atgtttttca ggaatggaat
    62941 gaagaatgtc aggccacagt aaaccgcgta aacgaagaag acgttaaaag cgataagcca
    63001 gatggttttg tctctcagca ccgaggtcat actggtgttt tgcgatgctc cgtcttcttt
    63061 tttcacctca acggacggtg cctcttcttt gtcattcagg acaaagaaaa taataatgcc
    63121 tacggcaatc acaataaggg aatagaacca gatgcctgct ttaaaaccca ataagccact
    63181 gccaaaccag gtaaatactg ccaacgcaga aaatgccacc acggtatcga caatgccacg
    63241 ccctgtttcg aagaagccaa acaaccgacc ttgttgttcg ctattgccca atcgacttac
    63301 cgacttgagc aagaccggcc agttcatcat gtcgcaagta acgccaaata gcgcccagac
    63361 aaagaggatg ccccaatacc ccggcattgt cgtcaaataa acacccagta atcctgtcgc
    63421 aatgagtgag aatgacatgg tgtatctgcg cggtagttta tcggcaaaat aaatagacag
    63481 aaaaaaaccc actgtcgtga caaatgagtt taccgacata gcattaccaa tttgaccatt
    63541 ggtcaaatgg aaatattcct gcatcgggat ataaaacgca tctttcagcg acggtaattt
    63601 ataaattgta ccgccacaca atgttaatag actaaataac gcccatttct ttttcgtgag
    63661 cataacatcc cctaagcgat ttttaaaatc actgttttaa aggcaataag ataccgacca
    63721 ttcatatgga atggttttat taactgcgta tgagttaaaa cttattagtg gttcagaaga
    63781 tggtttgcct cttgtttgat ttgttgtagt acgttacgca catgatttat ctgattaatt
    63841 gcatcttctt tttctttacg taaccgcgca tccagtaaat gattttctgg caatggtgtt
    63901 tcactcatct ggcgataagg gatccacgga ttatcatctt cgtcttcaaa gcggaacgcc
    63961 ggcgcatagt aatcaacctc ttcttccagg ccatatgccg tcacctgcgg ctcatcatca
    64021 cccaggcata taaggtgcgt taataacgct ttgaacggca tatcaccctg acctgaaata
    64081 caggctttat gacccaggcc acccggttct ttaacgatca aggcatcttt gatatggacc
    64141 tgggtaatat gcggcgccat cgtttttaaa gcgtcgatgg gatgctcatt tgcattgatc
    64201 atgttcgcaa aatcaaacag taaggaaaga gattccatct cactttcttt gaccagcgac
    64261 accagctcat ggctttttaa atcttcatgc tgttcgatcg taaaagtcag gccgctgtcc
    64321 tgatacgttt cccgtacata ggcaatgtcg ttagcgatga tcgataatac gtcgcgcaaa
    64381 ttaccttcat aacgtggata aaaacgtacg gacgatgccc cagttttcaa cgcaatggcg
    64441 acggcttcgt caatagatgc cttatctgag gcgctggttt caatatgaat atcaaggttc
    64501 agacggcggg ctttatctcc aaaggcagag agttctttat cgtccatatt accaagagaa
    64561 aaacgctcgc catccagaac atggattttg acgccacgta gattattttc acttgcgata
    64621 tcaagtaaat cagcaggcaa cactctttcc agccgcatat ttaaatgaaa ggcataagca
    64681 tgtaaataaa gaggaagagt gtcgacacgt tgtagaatct ttcttgcgtt atttattgtc
    64741 accataaaac acctacagat taatgatgag gtctacggcc tcaaatcgcc aatattcaat
    64801 atctatctgg acaatattgc cttgctggtc ggcgcgatgc ttttcaatta agatcgctgg
    64861 catacccgca gaaccgccaa gatacttgct gatatcgccc ggcattctga cgggtttgaa
    64921 aatcaatttt tttactaccg tctctttttg gtagcacttt tcccagactg cagaaaatga
    64981 gtggttttca agttgttcaa taaaacccgg agcaacttca ggattaatat atgtttcgtg
    65041 atagaaaact ttatgtcctt ccagcgcccc ccagccggtg atgcgataga gttcagttga
    65101 tggtgtcact gcaatcagtg tcgcgagcgt ctcgggaata tcgctggtac ggtttttctc
    65161 ggtaaacccc caggaaggct ctcgtccttg ctcaattgcg gcccgctgaa agctcgccga
    65221 caactccgga ctgtaattaa atcgcggctg ggtcacgaac caccccttac gatccttacg
    65281 gaagattttg gattctgcct cgaggttcaa caacgcctgg cgcagcgtca tacgtttaat
    65341 gcccagcagt tctcccagct ctctttctga agggagtttt ccacccggcg tggtgatgcc
    65401 atcttttaac caccgatcca gtttctcttt tgcattttct acggtggatt gatttcccgt
    65461 cattgtttaa tccttttggt ttaaaccaga tgaggcatct tacgcttacg ccaaaaaaga
    65521 aagcaattaa cggcgtatga ctttgcagat aattgtgaca aagctctcta ttgacgtaat
    65581 cccagactta taagggagat actcgcattg gtgtgcgctc acgacaggta aaaaaaaacc
    65641 tgccagcgat ggcaggtttt ttataactaa aaaacgaaat taatcgtctt tcggtgcgcg
    65701 gttggcgcgg cgacgatcgt tttccgtcag gtgacgttta cgaatacgga tagaggtcgg
    65761 agtcacttct accagttcgt catcatcgat gaactccaga gcttgttcca gagtcatgcg
    65821 gataggcgga accagaacaa cggcttcgtc agtaccggaa gcacgcatgt tggtcagttt
    65881 cttaccggtc aggcagttta cagtcaggtc gttagagcgg ctatgaatac cgataatctg
    65941 accttcgtaa acttctgcac cgtgaccgag gaacagctta ccgcgatcct gcagaccgaa
    66001 cagcgcgaac gcgaccgctt taccctgacc gttagagatc agtacgccgt tctgacgctg
    66061 acccacttca cccggacgta cgtcgtcgta gtggctgaag gtggagtaca gcagaccagt
    66121 accggaagtc atggtcatga actcagaacg gaagccaatc agaccacggc ttgggatcac
    66181 gtagtcgaga cgtacgcggc ctttaccgtc tggattcatg tttttcaggt cgcctttacg
    66241 ttcgcccagc gcctgcatta cagaaccctg atgctgttct tcaacgtcca gagtcacgtt
    66301 ttcatatggc tcttgtttac gaccgtcgat ttcacggaag ataactttcg gacgggatac
    66361 cgccagttcg aaaccttcac gacgcatgtt ttcgatcaga acagacaggt gtagttcgcc
    66421 acgaccagaa acgcggaacg catcagcgtc ttcggtttct tctacgcgca gcgcaacgtt
    66481 gtgtaccagt tctttgttca gacgatccag gatctgacga gacgttacga acttaccttc
    66541 tttaccgcag aacggcgagg tgttaacgca gaagaacata gaaacggtcg gctcatcaac
    66601 ggagagtgcc ggcagcgctt caacgttttg cgtgtcgcaa acggtgtcag aaatgttcag
    66661 ttcgccaagg cccgtgatcg caacgatatc gccagcttcc gccaggtcgg tttcgatacg
    66721 ttccagaccg aggtggccca gcactttacc gactttcgcg ttgcgggttt tgccttcgct
    66781 atcgatgata gtgacctgct ggttcggctt cactttaccg cgcttgatgc ggccaatgcc
    66841 gataacgcca acatagctgt tgtaatcgag ctgagaaatc tgcatctgga acggaccgtc
    66901 aaggtcaacg tccggcgcag gaacgtggtc aacaatcgcc tggtacagcg gggtcatgtc
    66961 ttccgccata tcttcgtggt ccagacccgc gataccgttc agcgcagaag cgtaaacgat
    67021 cgggaagtcc agctgctcgt cggtcgcgtc gaggttaacg aacagatcga atacctgatc
    67081 cacaacccaa tcaggacgcg cgccagggcg gtcaactttg ttgataacaa caatcggctt
    67141 caggccgtaa gcaaacgctt ttttggttac gaagcgcgtt tgcggcatcg ggccgtcaaa
    67201 tgcgtcaacc accagcagca ctgagtctac catggacatt acacgttcaa cttcaccacc
    67261 gaagtcggcg tgccccgggg tatcaacgat gttgatacgg taatcattcc atttgatagc
    67321 ggtgtttttc gcgaggatgg taatcccacg ctctttctcc aaatcgttgg agtccatcac
    67381 gcgctcttgg gtttcggcac gagagtcgaa cgtaccggat tgttggagca gcttgtctac
    67441 cagggtggtt ttaccatggt ctacgtgcgc gatgatggcg atattacgca atttttcgat
    67501 cacaactttg cctcaggcat tagaaatagc gcgttattgt acacggatta atcgcactac
    67561 aaaacaggat cacaaacatc ctccgcaaac aagtattgca gagtcccttt gtgatcgctt
    67621 tcacggagca taaaaagggt tatccaaagg tcattgcacc aacatggtgc ttaatgtttc
    67681 cattgaagca ctatattggt gcaacattca catcgtggtg cagccctttt gcacgatggt
    67741 gcgcatgata acgcctttta ggggcaattt aaaagttggc acagatttcg ctttatcttt
    67801 tttacggcga cacggccaaa ataattgcag atttcgttac cacgacgacc atgaccaatc
    67861 caggagagtt aaagtatgtc cgctgaacac gtactgacga tgctgaacga gcacgaagtg
    67921 aagtttgttg atttgcgctt caccgatact aaaggtaaag aacagcacgt cactatccct
    67981 gctcatcagg tgaatgctga attcttcgaa gaaggcaaaa tgtttgacgg ctcctcgatt
    68041 ggcggctgga aaggcattaa cgagtccgac atggtgctga tgccagacgc atccaccgca
    68101 gtgattgacc cgttcttcgc cgactccacc ctgattatcc gttgcgacat ccttgaacct
    68161 ggcaccctgc aaggctatga ccgtgacccg cgctccattg cgaagcgcgc cgaagattac
    68221 ctgcgttcca ctggcattgc cgacaccgta ctgttcgggc cagaacctga attcttcctg
    68281 ttcgatgaca tccgtttcgg atcatctatc tccggttccc acgttgctat cgacgatatc
    68341 gaaggcgcat ggaactcctc cacccaatac gaaggtggta acaaaggtca ccgtccggca
    68401 gtgaaaggcg gttacttccc ggttccaccg gtagactcgg ctcaggatat tcgttctgaa
    68461 atgtgtctgg tgatggaaca gatgggtctg gtggttgaag cccatcacca cgaagtagcg
    68521 actgctggtc agaacgaagt ggctacccgc ttcaatacca tgaccaaaaa agctgacgaa
    68581 attcagatct acaaatatgt tgtgcacaac gtagcgcacc gcttcggtaa aaccgcgacc
    68641 tttatgccaa aaccgatgtt cggtgataac ggctccggta tgcactgcca catgtctctg
    68701 tctaaaaacg gcgttaacct gttcgcaggc gacaaatacg caggtctgtc tgagcaggcg
    68761 ctgtactaca ttggcggcgt aatcaaacac gctaaagcga ttaacgccct ggcaaacccg
    68821 accaccaact cttataagcg tctggtcccg ggctatgaag caccggtaat gctggcttac
    68881 tctgcgcgta accgttctgc gtctatccgt attccggtgg tttcttctcc gaaagcacgt
    68941 cgtatcgaag tacgtttccc ggatccggca gctaacccgt acctgtgctt tgctgccctg
    69001 ctgatggccg gtcttgatgg tatcaagaac aagatccatc cgggcgaagc catggacaaa
    69061 aacctgtatg acctgccgcc agaagaagcg aaagagatcc cacaggttgc aggctctctg
    69121 gaagaagcac tgaacgaact ggatctggac cgcgagttcc tgaaagccgg tggcgtgttc
    69181 actgacgaag caattgatgc gtacatcgct ctgcgtcgcg aagaagatga ccgcgtgcgt
    69241 atgactccgc atccggtaga gtttgagctg tactacagcg tctaatagtt gaagttgtac
    69301 tacccggcgc aacaacgccg ggatttagtt gccgtggaaa tttcagccca tctctgcatg
    69361 ggcttttttc tccgtcaatt ctctgatgct tcgcgctttt tatccgtaaa aagctataat
    69421 gcactaaaat ggtgcaacct gttcaggaga ctgctttatg gcaacaggca cgcagcccga
    69481 tgctgggcag atcctcaact cgctgattaa cagtattttg ttaatcgatg acaacctggc
    69541 gatccattac gccaaccctg ccgcgcaaca actgctcgcc caaagctccc gcaaattgtt
    69601 tggtacaccg ttaccggaac tgttgagcta cttctcatta aatatcgagc tgatgcaaga
    69661 aagtctggag gcggggcaag gttttaccga taacgaagtg acgctggtca tcgacgggcg
    69721 ctcgcatatc ctttctgtga cggcccagcg tatgccggac ggcatgatcc tgctggagat
    69781 ggctccgatg gataaccagc gccgcttaag tcaggaacag ctacagcacg cccagcaggt
    69841 tgctgcccgt gatttagtgc gcggcctggc acatgagatt aaaaatccgc ttggcggttt
    69901 acgtggcgcg gcgcagctgc tcagcaaagc gttacctgac ccatcactac tcgaatatac
    69961 caaagtgatt atcgaacagg cggaccggct gcgaaatctg gtcgaccgtc tgttggggcc
    70021 gcagctgccc ggtacgcgcg ttaccgaaag tattcacaaa gtggctgaac gcgtggtaac
    70081 gctggtgtcg atggaactgc cggacaacgt gcggttgatt cgtgattacg atcccagcct
    70141 gccggaactg gcgcacgacc cggatcaaat tgaacaggtc ttgctgaata ttgtgcgcaa
    70201 tgcgctacag gcgctggggc cggaaggcgg tgaaatcatt ctgcgtaccc gcaccgcgtt
    70261 tcaactgacc ttacacggcg agcgctaccg gctggcggcg cggattgatg tggaagataa
    70321 cgggccgggc attccgcctc atttgcagga tacgctgttt tacccgatgg tcagcggccg
    70381 cgaaggtggc accgggcttg gcttatccat cgctcgtaat ttgattgatc agcattcagg
    70441 caaaattgaa tttaccagtt ggccagggca taccgagttc tcggtttacc tgcctatcag
    70501 gaaataaagg tgacgtttat gcaacgaggg atagtctggg tagtcgatga cgatagttcc
    70561 atccgttggg tgcttgaacg tgcgctcgct ggggcaggtt taacctgtac gacgtttgag
    70621 aacggcgcag aagtgctgga ggcgctggcg agcaaaacgc cggatgtgct gctttcagat
    70681 atccgtatgc cgggaatgga cgggctggcg ctgctcaagc agattaaaca gcgccatcca
    70741 atgcttccgg tcatcattat gaccgcacat tccgatctgg atgctgccgt cagcgcctat
    70801 caacaagggg cgtttgatta tctgcccaaa ccgtttgata tcgacgaagc agtggcgctg
    70861 gttgagcgcg ctatcagtca ttaccaggaa cagcagcagc cgcgtaatgt tcagcttaac
    70921 ggcccaacga ccgatatcat cggcgaagcg ccagccatgc aggacgtgtt ccgtattatc
    70981 ggtcggcttt cgcgttcttc tattagcgtg ctgattaacg gcgaatccgg caccggtaaa
    71041 gaactggtcg ctcatgccct gcatcgccac agtccgcgcg ccaaagcgcc gtttatcgcg
    71101 ctgaatatgg cagctatccc aaaagatttg atcgaatcag aactgtttgg ccacgagaaa
    71161 ggcgcgttta ctggcgcgaa taccattcgt caggggcgtt ttgaacaggc cgatggcggt
    71221 acattattcc tcgacgaaat tggtgatatg ccgctggatg tgcagacgcg tttgctgcgc
    71281 gtgctggcag acggtcagtt ttaccgcgtt ggcggctatg cgccggtgaa agtggatgtg
    71341 cggattatcg ctgccactca ccagaatctc gaacagcgag tgcaggaagg taagttccgt
    71401 gaggatctgt tccaccgcct gaacgttatc cgcgttcatc tgccgccgct gcgcgaacgt
    71461 cgggaagata ttccccgtct ggcgcgccat tttttacagg ttgccgcgcg cgaactgggc
    71521 gtagaagcga agttactgca tccggaaacc gaagctgctc tgacgcgtct ggcgtggcca
    71581 ggcaacgtgc gccagctgga aaacacctgc cgctggctaa cggtgatggc cgccgggcag
    71641 gaagtgttga ttcaggattt gcccggcgaa ctgtttgaat caacggttgc ggagagtact
    71701 tcgcaaatgc aaccggacag ttgggcgacg cttcttgcgc agtgggcaga cagagcgctg
    71761 cgttccggtc atcaaaatct gctttccgaa gcgcagccag agctggagcg gacgttactg
    71821 acgaccgcgt tgcgacatac gcaggggcat aaacaggaag cggcgcggct actcggctgg
    71881 ggccgcaaca ctctgacgcg taagttaaaa gagctgggga tggagtgatt cacggcgtgt
    71941 gtgtaaagat tgattattga gcgcaaattg ctggtatttt gcgctttact gttccgataa
    72001 gttcagtatg atcttgcccg gaaaacgggg agagtcatta tgctggaatc aataattaat
    72061 ctggtatcga gtggcgcagt tgacagccac acaccgcaaa ctgccgttgc tgccgtgctg
    72121 tgtgccgcaa tgattgggct gtttagttga gtttcagatt gctgatgaag tgctctttgt
    72181 tcatgccgga tgcggcgtga acgccttatc cggcctacaa aagcaatatt ttcaacaggt
    72241 tttgtaggcc tgataagcgt agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc
    72301 tttcgttttc tactttgtaa acgaagcgcc attcactatt taaatcaccc gtgagaactg
    72361 ctgcatccgc gctttctggc gcagataggt atcaaagcac atgcaaatgt tgcggatcag
    72421 caagcgacct ttcgccgtca cctgtattcc cttctcatcc acatccacca gcccatcttt
    72481 tgctaacggg gcgagcagct tgagatcttc cgcaaagtaa tcagcgaagt gcaaatccca
    72541 ctgtttctca ataggggcgt aatccagacg gaagttgcag atgagcgact taatcacatc
    72601 gcggcgaata cagtcatcac gcgttagcgc aataccacgc cacagcgcat tgccttgttc
    72661 atccacttgc tgatagtact gcttcaactc tttctggttc tgcgcgtagc agtcgccaat
    72721 catgctgatg gcggaaacgc ccatccccag cagatcggta tcgccctgag tggtgtagcc
    72781 ctggaagtta cgatgcagca cgccttcacg ctgggccacc gccagctcgt catccggacg
    72841 ggcaaagtga tccataccga taaactgata gcccgattgc gtcaggaagg cgatggtttc
    72901 ctgcaggata tcgagttttt gctgcggact cggcaggtca gcatctttga ttttgcgctg
    72961 agcagcaaaa atggtcggca gatgcgcgta gttaaagaca ctcagacgat cggggttcag
    73021 ctccgccaca cgtttcaggg taaaggcgaa actctccggc gtctgtttcg gcaggccgta
    73081 aatcaggtcg atgttggtgg aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa
    73141 gatgaactct tcatcctgct cgcggttaac cagacgttgc acttctttgt tgaagtcctg
    73201 cacgcccatg ctcaggcgat taaagccttc ggcgcgtaaa tgatcgagta catccagttc
    73261 gatttcccgc ggatcgactt cgatcgaaat ctccgcatcg gcattgaact ggaagttttc
    73321 gcgcagcagc ttcatcaggc ggctgatttg cgctttattc agatacgtcg gcgttccgcc
    73381 gccccagtgc aattggctga cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc
    73441 ttgctccagc gcgtccagat actgatcggc cttgtgctgc tggcgagtaa caatcttatt
    73501 gcaaccgcag aagtaacaaa gcttatggca gaacgggata tgtacgtaga gagataatgg
    73561 acgctcagga tagcgcgcca cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa
    73621 ctccagcgcg gtcgggtacg aggtgtatcg tggcccggaa tagttatatt tctggatcag
    73681 ggccagatcc cagtcgattt gctgtacaga catgctcact ccttccgatg gcgtctctgg
    73741 cggctacggc gtaccggcgg atgcccgttt cgggtcatca gccggttgcg cagccacttt
    73801 tgtcgccgcg acaaccgctg tagtttaacg aataaccaca ccagataaca tataaccgga
    73861 agggttatca gcagaacggg aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga
    73921 cgcatcatgt cttcttgctt ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg
    73981 ccgagtttct gcatcaactc atcaatacga tccagtttag catccaccca ggattgctct
    74041 tcggcgctca gggtttcgcc tgcttccaga cgttccagca gcgcatccag acgctcatcc
    74101 gtttccagta attccaactc cgcctgcggt gaaagcatag gtttctcact cttcggtttg
    74161 tgctgtttgg tgactttttc agtcacgccc aatggaatag gggttttact gccaatacgt
    74221 ggatcttttg gtgcgttctg gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg
    74281 ctgcccggcg catgaccacg acgttttttc tgacgcttgc gatcacgagc ttcctgatcc
    74341 agctcctcgc gtgtttttcg acgcgctttt gcatgacctt tgctgcgtga gtttgaagat
    74401 gatggtttca taatggttcg tcttttagcc gttttattca gtatagattt gcggcggaat
    74461 ctaacagaaa gcaagcaaag aaaaaaggcg acagattact ctgtcgcctt ttttcctgac
    74521 tcataaccct taacgggtct tacaatcctt gcaggccgcc aacacaccct gtttttcctt
    74581 tagccggaac aatccgtgtt gattccattt ccgtttaatt acgtctccgg acgtttgtct
    74641 tcctgacaat cctctgtctt cgcctcctgg cgctcctgac cctcatcctg agtcattgtt
    74701 cctgttagcg tcctcgctct atgggtgcta ctttacgcct ttgctttaaa caaacaatca
    74761 accgccatca gtatttgcac attttcagag ttttatataa aagcaattca ctgatttata
    74821 tagcttattt gacatgcatc gcagaatttt ctctgctatt tcgcataaag actatcggca
    74881 atgtctcaca aagcgtatgg cttttactta caacgctcac gccgaaaaat ccgcctttta
    74941 ttatggttca ggtataatcg ccacaaagct ttgactgacg gagacgaccg ctttgactaa
    75001 tttgaattat caacagacgc attttgtgat gagtgcgcct gatattcgcc acctaccttc
    75061 cgataccgga attgaagtgg cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct
    75121 gaacacgctg actaaccaga aaagcctggc tcgtacctca aaaaccccag ggcgcaccca
    75181 gcttatcaac ctgtttgaag tggctgacgg caagcgtctg gttgacttgc ctgggtacgg
    75241 ttatgcggaa gtcccggaag agatgaagcg caaatggcag cgtgcgctcg gcgaatacct
    75301 cgaaaaacgt cagagcctgc aaggtctggt ggtgctaatg gatattcgcc atccgctgaa
    75361 agatttggat cagcagatga ttgagtgggc ggtagacagc aatatcgccg ttctggtgct
    75421 gctgaccaaa gcggacaaac tggcaagcgg cgcacgtaaa gcgcaattga atatggtgcg
    75481 tgaagctgta ctggcgttta acggtgatgt gcaggttgaa acgttttctt cgttgaagaa
    75541 acaaggcgtg gacaagctgc ggcagaaact ggatacctgg tttagcgaga tgcagcctgt
    75601 agaagaaacg caggacggcg aataattttc ttgccttaat gcttgtgccg gatgtggcgt
    75661 atccggcccg taaattcaat agattacaat tctaccgttg gcctgataaa catagcgcat
    75721 caggcattac ggatcttttc tttcgcccaa taaaaaacgc cccagtcatt actgactggg
    75781 gcggctaaaa tattcagcca aatccgatta cgtgaagtaa aaggtctgaa agatagaaca
    75841 tcttacctct gtaccctacg cgactaactt tactcttttt tgttcagttc agaaagcact
    75901 ttttgtaatt ttttttcagt tgttgcatag gaatttctaa tagccatcac aaaacgtgac
    75961 agcttatgtt gcttacttac gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat
    76021 cccagttttc gccactcccc acttccacca gcaacggcac atccagacgg gtacagtttt
    76081 ccatcagttg atgaatctgc ttcgcgacgg catcaacatc atctttatga acttcaaata
    76141 ccagttcatc gtgtacctgc atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg
    76201 catcaacggc aatcatcgcc cgtttgataa tgtcggcggc ggttccctgc attggcgcgt
    76261 taatggctgc acgttcagcc gctgcacgac gagcaccatt gctggatttg atatccggca
    76321 gatacagacg gcgtccgtcc agcgtttcaa cgtagccctg ctctttcgcc tgagcacggg
    76381 tgcgttccat atactccagc acgccagggt agcgttcgaa gtaaaggtcc atgtacttct
    76441 gcgcttcttt acgtggaatg ttcaattgcc gcgccagacc gaaagcactc atgccataaa
    76501 tcagaccaaa gttgatcgct ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg
    76561 gcaaaccaaa cacttctgcc gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg
    76621 tcagcaagcc tttgtcacgc gagagatgcg ccataatgcg cagttcaatc tgcgagtagt
    76681 ccgctgagac aatcacataa tcctctggcg caataaacgc ctggcggata cgacgacctt
    76741 cttcgttacg caccggaatg ttttgcaggt taggatcggt tgacgataaa cgtcccgttg
    76801 cagttactgc ctggtgataa gaggtatgca cacgcccggt tttcgggttg atcatcagcg
    76861 gcagcttgtc ggtgtaggtc gatttcagct tcgccagacc acgatactcc agaatcactt
    76921 ttggcaacgg atagtccagc gccagttctt ccagtacctc ttccgacgtt gacggcgcgc
    76981 cacccggcgt tttcttcagc ggtttaatac cctgtttttc aaacagaatg gtttgcaact
    77041 gcttggtgga agaaaggtta aattcttcac ctgcaatttc atgcgctttc ttttccagct
    77101 cagccagacg caaggtgagc tcttcagaat gattgtgcag cactttcgga tcgatcttca
    77161 caccgttacg ttcaatgcgt gaaagcaccg gcaccagcgg catttcgata ttctcgaaga
    77221 cgttcaacgg ccctttgtgt ttttgcaggt ccggccacat tttcagatgc aactgcaagg
    77281 tgacatcagc atcttcggcg gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg
    77341 tcagttgatt tttgccttta cccgcaatct cttcaaaagt gatggttttg tgcttcaacc
    77401 aacgttccgc gaggctgtcc atatcgtgac gcccggcaac gctattgaga atgtaggact
    77461 ccagcatggt atcaaacgca atcccacgca gctcaatgcc gtaattcgcc agaataccgc
    77521 gatcgtattt caggttttgc ccgaccttca gcgccttttc atcttccagc agcggtttta
    77581 gcaactcgag tgcacgctcg cgagagattt gatcgggcgc atcaagataa tcatgagcaa
    77641 ccggaatata tgccgctacg cctggctcga tagcaaaaga aagcccgacc aggttagcag
    77701 agatgttatc aaggctgtcg gtttcggtat caaatgcaaa taccggcgct ttttccagct
    77761 tcgcaatcca cgctttcagt gtttcttcat caaggatggt gacgtagttg tcataagaaa
    77821 tcaccgttgc cgtcacttct ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg
    77881 ctggttttgc ccctttggcc tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt
    77941 tgaactcata ctttttgaac agccccaaca actcttccgc tgccggttgc tgcacttcca
    78001 gttgttcaca ggtcagctcc agttcaacgt cggttttaat cgtcgccagc tggtatgaga
    78061 gataagcaac ttctttgttt tgctcgagct tcgctgccat tgttttcgcg ccacggaagc
    78121 tcaacccagc aattttttct ggctcggcat acagcgtatc cagcccgccc agaccttgca
    78181 gcaatgcctg cgcggttttt tcaccgacgc ccggtacgcc agggatgtta tcggaggagt
    78241 cacccatcag cgccaggaaa tcgatgatca gttctggcgg cacgccgtac ttattcacca
    78301 cctcttccgg tccgaggatg gtattcgtca tggtattgat aagcgtaata tttggcgtca
    78361 ccagctgcgc catatcttta tcgccagtgc tgatcagcac cggacgcccg gctttttcgg
    78421 cttcgcgcgc cagagtaccg ataacgtcgt ccgcttctac gccagaaacc gccagcagcg
    78481 gcagtcccat cgctttaacc atcgcgtgca agggttcgat ttgtgcacgc agatcgtccg
    78541 gcattggcgg gcgatgtgat ttgtaatgtt caaacagttc atcacgaaag gtttttccct
    78601 tggcgtcaaa gaccaccgct gcatgcgtcg gtttatattg catgatcaga ctgcgcagca
    78661 tgttgaggac accatacatc gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa
    78721 acgcgtgata tgcgcgataa agataagatg aaccatctac aaggataagt ggattttggg
    78781 ggatctgaac cataatgtcc gtgcctgttt atcagattat gggtaaagga tgccacagac
    78841 aggatgaaaa catgagtttt tcaccgcttt tcgtgaaaat cttttgtaga tcttctggat
    78901 cgctcgcaaa tcgtcatgtg gataactttg tgcataaatt atttacatcg cttttaattt
    78961 attatcatgc tttttacagc atgatgtttg tctttgtttt tcatgcagtt aaaaataaat
    79021 ccatccctaa taattgagat aatgggattt tttctaatgt ggatagatat gaattatttt
    79081 tctccttaag gatcatccgt tatttgggtc gttttctgct aaaatctgcg ccttgcgctt
    79141 ctttagcgca ctgtctatgg ctaacctttt gaataaattc attatgacga gaatactcgc
    79201 tgcgataacc cttttgctga gtatcgtatt gaccattttg gtcactattt tttgttccgt
    79261 cccgatcatc attgccggga ttgtaaaact attgctacct gtgccagtca tttggcgaaa
    79321 ggtttcgcgt ttttgtgatt ttatgatgta ttgctggtgt gaaggtctgg cggtattact
    79381 gcatctgaac ccacacctgc aatgggaagt tcacgggctg gaagggttaa gtaagaagaa
    79441 ctggtatctg cttatttgta accaccgtag ttgggcagat attgtcgtac tgtgcgtgct
    79501 gtttcgtaag cacattccga tgaataaata tttcctcaag caacagctgg cctgggtgcc
    79561 atttcttggc ctggcgtgct ggtcgctgga tatgccgttt atgaagcgtt attcccgcgc
    79621 ttatttatta cgtcatccgg agcgacgtgg taaagatgtt gaaaccactc ggcgttcttg
    79681 tgaaaagttt cgcctgcatc ccaccaccat tgtcaatttc gttgaaggct cccgtttcac
    79741 gcaagaaaaa catcagcaaa cccactccac ttttcaaaac ttgttgccac caaaggcggc
    79801 aggcattgcg atggcgctaa atgtactggg caaacaattc gataaactgc ttaatgttac
    79861 actgtgttat ccggacaata accgtcagcc gttcttcgat atgttaagcg gtaaactgac
    79921 gcggattgtc gtacatgtgg atttacagcc tattgccgat gagttacatg gcgactacat
    79981 caatgacaaa agctttaagc gccattttca gcaatggctg aactcattgt ggcaggaaaa
    80041 agaccggctt ctgacgtctc tcatgtcatc acagcgtcag aataagtaag ttttactcgc
    80101 ctctgaatga gcagaggcga gtgagtattt taatgaacaa aacgtccggc acgagacata
    80161 aattcttctt cgctcatctc ctgtccgtta aaaacaactt tacccggtgt ataacgcagc
    80221 tgcagcgagg cggtattgtt gtcaatcgtg attaactgaa acatctgacc caatgtcatc
    80281 atcccgctga tttgtttatc agcctgcttt tgcgcttttt ccgcatccat tccttcagat
    80341 aaattaagct gttttgcggt ttctgtgacg acattaagcg gtaacttcac atcaaaattg
    80401 agcgatttga tatctttgtt tgtggatgat gaagatttgg ctgggtcggc aatactgata
    80461 tccagattgg catttagttc gccgagtgcg ttcttccatc ttaccggttg tttaatggtc
    80521 ggctcacttt tttgtaataa cggtaaatac tctttgaaca atgcagcatt cacttcttgc
    80581 agagcaactt catcgtttgc taactcaggg tgtacagcaa gctgcttctg catagcaatg
    80641 ttatactgga tgataaattg gcgcactgct gagggatcaa tagattcagc aatcaatgac
    80701 cactcaccac taccgagttg ctgattccct tttgttaact tatcaagttc gtaggtcaga
    80761 ttgacattga tataatcttt gtctttaacg cgatccagca tggtttttgc aacgaaagca
    80821 tcgatttggg caacgtcttc cccgtgattg ataatgttaa tctgattaaa tttcgactcg
    80881 ctttcaccaa ccggaaattt cttttcttct aatcgcgcca gagaatcaat ggtaaaactt
    80941 tttgccgtta ctttgttcat tggtgataat tgcagaacaa tatcttcgac tttgccttca
    81001 atatccaggt tagacaatga actaccatca ccattaaaag taaactggcc ctcaccaaaa
    81061 gaaaactttc catactctgc aggggcaaca tctaatgtcg tagagaatga gccaccaaac
    81121 gcgaatcgca atgtcgcttc cacgggcgat ttatttttcg cggcgataaa tagcggttgt
    81181 gtcagttcat tattcactaa gttcactttt gccgctgcca gtgctgggat gacattacca
    81241 tgcattaaca ttgtgatggg caacggaccg tgctcaacat ccacatcaaa tacaactttc
    81301 tggcctggct tgatattgag atcgggtgca ccgttatcga atgtcatttg catttgaaaa
    81361 cgggaattaa aaaagccttt gtcaaaattt tgataactta acgtcatatc gtaggcatgt
    81421 tcacctttct ttttagcatc gttaaaatct ttaataaatt tttcgacacc aggctgaatt
    81481 tgtgtacctg tgtaccatgt gccaccaccc cagattacgg ctaacgcaac aataacacct
    81541 gtagctgact tacgtatcat ataaatattc cctgataaaa cagcaaaact ttgaatatcc
    81601 acttatgctg aaataatgtc cagcggcagg atg
//
LOCUS       DAZGAN020000007        81161 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGAN020000007 DAZGAN020000000
VERSION     DAZGAN020000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 81161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.007"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            8..562
                     /gene="ybeT"
                     /locus_tag="ACHKYO_RS02720"
                     /old_locus_tag="pgaptmp_000544"
     CDS             8..562
                     /gene="ybeT"
                     /locus_tag="ACHKYO_RS02720"
                     /old_locus_tag="pgaptmp_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4427440.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLRVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(572..1999)
                     /gene="djlB"
                     /locus_tag="ACHKYO_RS02725"
                     /old_locus_tag="pgaptmp_000545"
     CDS             complement(572..1999)
                     /gene="djlB"
                     /locus_tag="ACHKYO_RS02725"
                     /old_locus_tag="pgaptmp_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HGU4427441.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEDIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDKGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1996..2703)
                     /gene="ybeR"
                     /locus_tag="ACHKYO_RS02730"
                     /old_locus_tag="pgaptmp_000546"
     CDS             complement(1996..2703)
                     /gene="ybeR"
                     /locus_tag="ACHKYO_RS02730"
                     /old_locus_tag="pgaptmp_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4427442.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRNYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWHSPEDDNLQLLREFLARKEYDDSGNDMFYQLFASDNAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2867..3844
                     /gene="ybeQ"
                     /locus_tag="ACHKYO_RS02735"
                     /old_locus_tag="pgaptmp_000547"
     CDS             2867..3844
                     /gene="ybeQ"
                     /locus_tag="ACHKYO_RS02735"
                     /old_locus_tag="pgaptmp_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HGU4427443.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3914..4396)
                     /gene="ybeL"
                     /locus_tag="ACHKYO_RS02740"
                     /old_locus_tag="pgaptmp_000548"
     CDS             complement(3914..4396)
                     /gene="ybeL"
                     /locus_tag="ACHKYO_RS02740"
                     /old_locus_tag="pgaptmp_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HGU4427444.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4631..7213
                     /gene="leuS"
                     /locus_tag="ACHKYO_RS02745"
                     /old_locus_tag="pgaptmp_000549"
     CDS             4631..7213
                     /gene="leuS"
                     /locus_tag="ACHKYO_RS02745"
                     /old_locus_tag="pgaptmp_000549"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGU4427445.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7228..7809
                     /gene="lptE"
                     /locus_tag="ACHKYO_RS02750"
                     /old_locus_tag="pgaptmp_000550"
     CDS             7228..7809
                     /gene="lptE"
                     /locus_tag="ACHKYO_RS02750"
                     /old_locus_tag="pgaptmp_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HGU4427446.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7809..8840
                     /gene="holA"
                     /locus_tag="ACHKYO_RS02755"
                     /old_locus_tag="pgaptmp_000551"
     CDS             7809..8840
                     /gene="holA"
                     /locus_tag="ACHKYO_RS02755"
                     /old_locus_tag="pgaptmp_000551"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGU4427447.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8842..9483
                     /gene="nadD"
                     /locus_tag="ACHKYO_RS02760"
                     /old_locus_tag="pgaptmp_000552"
     CDS             8842..9483
                     /gene="nadD"
                     /locus_tag="ACHKYO_RS02760"
                     /old_locus_tag="pgaptmp_000552"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGU4427448.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9507..10118
                     /gene="cobC"
                     /locus_tag="ACHKYO_RS02765"
                     /old_locus_tag="pgaptmp_000553"
     CDS             9507..10118
                     /gene="cobC"
                     /locus_tag="ACHKYO_RS02765"
                     /old_locus_tag="pgaptmp_000553"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HGU4427449.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10378..10695
                     /gene="rsfS"
                     /locus_tag="ACHKYO_RS02770"
                     /old_locus_tag="pgaptmp_000554"
     CDS             10378..10695
                     /gene="rsfS"
                     /locus_tag="ACHKYO_RS02770"
                     /old_locus_tag="pgaptmp_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGU4427450.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10699..11166
                     /gene="rlmH"
                     /locus_tag="ACHKYO_RS02775"
                     /old_locus_tag="pgaptmp_000555"
     CDS             10699..11166
                     /gene="rlmH"
                     /locus_tag="ACHKYO_RS02775"
                     /old_locus_tag="pgaptmp_000555"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGU4427451.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSTLTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11197..13098
                     /gene="mrdA"
                     /locus_tag="ACHKYO_RS02780"
                     /old_locus_tag="pgaptmp_000556"
     CDS             11197..13098
                     /gene="mrdA"
                     /locus_tag="ACHKYO_RS02780"
                     /old_locus_tag="pgaptmp_000556"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HGU4427452.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13101..14213
                     /gene="mrdB"
                     /locus_tag="ACHKYO_RS02785"
                     /old_locus_tag="pgaptmp_000557"
                     /gene_synonym="rodA"
     CDS             13101..14213
                     /gene="mrdB"
                     /locus_tag="ACHKYO_RS02785"
                     /old_locus_tag="pgaptmp_000557"
                     /gene_synonym="rodA"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HGU4427453.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14224..15312
                     /gene="rlpA"
                     /locus_tag="ACHKYO_RS02790"
                     /old_locus_tag="pgaptmp_000558"
     CDS             14224..15312
                     /gene="rlpA"
                     /locus_tag="ACHKYO_RS02790"
                     /old_locus_tag="pgaptmp_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HGU4427454.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPIGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15451..16662
                     /gene="dacA"
                     /locus_tag="ACHKYO_RS02795"
                     /old_locus_tag="pgaptmp_000559"
     CDS             15451..16662
                     /gene="dacA"
                     /locus_tag="ACHKYO_RS02795"
                     /old_locus_tag="pgaptmp_000559"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HGU4427455.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16773..17036
                     /gene="ybeD"
                     /locus_tag="ACHKYO_RS02800"
                     /old_locus_tag="pgaptmp_000560"
     CDS             16773..17036
                     /gene="ybeD"
                     /locus_tag="ACHKYO_RS02800"
                     /old_locus_tag="pgaptmp_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HGU4427456.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17137..17778
                     /gene="lipB"
                     /locus_tag="ACHKYO_RS02805"
                     /old_locus_tag="pgaptmp_000561"
     CDS             17137..17778
                     /gene="lipB"
                     /locus_tag="ACHKYO_RS02805"
                     /old_locus_tag="pgaptmp_000561"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HGU4427457.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            18037..18990
                     /gene="ybeF"
                     /locus_tag="ACHKYO_RS02810"
                     /old_locus_tag="pgaptmp_000562"
     CDS             18037..18990
                     /gene="ybeF"
                     /locus_tag="ACHKYO_RS02810"
                     /old_locus_tag="pgaptmp_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HGU4427458.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPTSDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19199..20164
                     /gene="lipA"
                     /locus_tag="ACHKYO_RS02815"
                     /old_locus_tag="pgaptmp_000563"
     CDS             19199..20164
                     /gene="lipA"
                     /locus_tag="ACHKYO_RS02815"
                     /old_locus_tag="pgaptmp_000563"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HGU4427459.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20265..20468)
                     /gene="tatE"
                     /locus_tag="ACHKYO_RS02820"
                     /old_locus_tag="pgaptmp_000564"
     CDS             complement(20265..20468)
                     /gene="tatE"
                     /locus_tag="ACHKYO_RS02820"
                     /old_locus_tag="pgaptmp_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HGU4427460.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20597..21385)
                     /locus_tag="ACHKYO_RS02825"
                     /old_locus_tag="pgaptmp_000565"
     CDS             complement(20597..21385)
                     /locus_tag="ACHKYO_RS02825"
                     /old_locus_tag="pgaptmp_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HGU4427461.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21478..21861
                     /gene="crcB"
                     /locus_tag="ACHKYO_RS02830"
                     /old_locus_tag="pgaptmp_000566"
     CDS             21478..21861
                     /gene="crcB"
                     /locus_tag="ACHKYO_RS02830"
                     /old_locus_tag="pgaptmp_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGU4427462.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21916..22125)
                     /gene="cspE"
                     /locus_tag="ACHKYO_RS02835"
                     /old_locus_tag="pgaptmp_000567"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21916..22125)
                     /gene="cspE"
                     /locus_tag="ACHKYO_RS02835"
                     /old_locus_tag="pgaptmp_000567"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU4427463.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22300..22860)
                     /gene="pagP"
                     /locus_tag="ACHKYO_RS02840"
                     /old_locus_tag="pgaptmp_000568"
     CDS             complement(22300..22860)
                     /gene="pagP"
                     /locus_tag="ACHKYO_RS02840"
                     /old_locus_tag="pgaptmp_000568"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HGU4427464.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23449..24834
                     /gene="dcuC"
                     /locus_tag="ACHKYO_RS02845"
                     /old_locus_tag="pgaptmp_000569"
     CDS             23449..24834
                     /gene="dcuC"
                     /locus_tag="ACHKYO_RS02845"
                     /old_locus_tag="pgaptmp_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU4427465.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24875..25555)
                     /gene="dpiA"
                     /locus_tag="ACHKYO_RS02850"
                     /old_locus_tag="pgaptmp_000570"
                     /gene_synonym="citB"
     CDS             complement(24875..25555)
                     /gene="dpiA"
                     /locus_tag="ACHKYO_RS02850"
                     /old_locus_tag="pgaptmp_000570"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HGU4427466.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(25524..27182)
                     /gene="dpiB"
                     /locus_tag="ACHKYO_RS02855"
                     /old_locus_tag="pgaptmp_000571"
     CDS             complement(25524..27182)
                     /gene="dpiB"
                     /locus_tag="ACHKYO_RS02855"
                     /old_locus_tag="pgaptmp_000571"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HGU4427467.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            27562..28620
                     /gene="citC"
                     /locus_tag="ACHKYO_RS02860"
                     /old_locus_tag="pgaptmp_000572"
     CDS             27562..28620
                     /gene="citC"
                     /locus_tag="ACHKYO_RS02860"
                     /old_locus_tag="pgaptmp_000572"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HGU4427468.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            28635..28931
                     /gene="citD"
                     /locus_tag="ACHKYO_RS02865"
                     /old_locus_tag="pgaptmp_000573"
     CDS             28635..28931
                     /gene="citD"
                     /locus_tag="ACHKYO_RS02865"
                     /old_locus_tag="pgaptmp_000573"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HGU4427469.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            28928..29836
                     /gene="citE"
                     /locus_tag="ACHKYO_RS02870"
                     /old_locus_tag="pgaptmp_000574"
     CDS             28928..29836
                     /gene="citE"
                     /locus_tag="ACHKYO_RS02870"
                     /old_locus_tag="pgaptmp_000574"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HGU4427470.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            29847..31379
                     /gene="citF"
                     /locus_tag="ACHKYO_RS02875"
                     /old_locus_tag="pgaptmp_000575"
     CDS             29847..31379
                     /gene="citF"
                     /locus_tag="ACHKYO_RS02875"
                     /old_locus_tag="pgaptmp_000575"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HGU4427471.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            31383..31934
                     /gene="citX"
                     /locus_tag="ACHKYO_RS02880"
                     /old_locus_tag="pgaptmp_000576"
     CDS             31383..31934
                     /gene="citX"
                     /locus_tag="ACHKYO_RS02880"
                     /old_locus_tag="pgaptmp_000576"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HGU4427472.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            31909..32787
                     /gene="citG"
                     /locus_tag="ACHKYO_RS02885"
                     /old_locus_tag="pgaptmp_000577"
     CDS             31909..32787
                     /gene="citG"
                     /locus_tag="ACHKYO_RS02885"
                     /old_locus_tag="pgaptmp_000577"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HGU4427473.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            32838..34301
                     /gene="citT"
                     /locus_tag="ACHKYO_RS02890"
                     /old_locus_tag="pgaptmp_000578"
     CDS             32838..34301
                     /gene="citT"
                     /locus_tag="ACHKYO_RS02890"
                     /old_locus_tag="pgaptmp_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HGU4427474.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            34415..35221
                     /gene="rna"
                     /locus_tag="ACHKYO_RS02895"
                     /old_locus_tag="pgaptmp_000579"
     CDS             34415..35221
                     /gene="rna"
                     /locus_tag="ACHKYO_RS02895"
                     /old_locus_tag="pgaptmp_000579"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HGU4427475.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            35451..35861
                     /gene="rnk"
                     /locus_tag="ACHKYO_RS02900"
                     /old_locus_tag="pgaptmp_000580"
     CDS             35451..35861
                     /gene="rnk"
                     /locus_tag="ACHKYO_RS02900"
                     /old_locus_tag="pgaptmp_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HGU4427476.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            35999..36088
                     /gene="yldA"
                     /locus_tag="ACHKYO_RS02905"
                     /old_locus_tag="pgaptmp_000581"
     CDS             35999..36088
                     /gene="yldA"
                     /locus_tag="ACHKYO_RS02905"
                     /old_locus_tag="pgaptmp_000581"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HGU4427477.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYFENHS"
     gene            complement(36092..37330)
                     /gene="ybdR"
                     /locus_tag="ACHKYO_RS02910"
                     /old_locus_tag="pgaptmp_000582"
     CDS             complement(36092..37330)
                     /gene="ybdR"
                     /locus_tag="ACHKYO_RS02910"
                     /old_locus_tag="pgaptmp_000582"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HGU4427478.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            37551..37979
                     /gene="uspG"
                     /locus_tag="ACHKYO_RS02915"
                     /old_locus_tag="pgaptmp_000583"
     CDS             37551..37979
                     /gene="uspG"
                     /locus_tag="ACHKYO_RS02915"
                     /old_locus_tag="pgaptmp_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HGU4427479.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(38100..39665)
                     /gene="ahpF"
                     /locus_tag="ACHKYO_RS02920"
                     /old_locus_tag="pgaptmp_000584"
     CDS             complement(38100..39665)
                     /gene="ahpF"
                     /locus_tag="ACHKYO_RS02920"
                     /old_locus_tag="pgaptmp_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HGU4427480.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(39794..40357)
                     /gene="ahpC"
                     /locus_tag="ACHKYO_RS02925"
                     /old_locus_tag="pgaptmp_000585"
     CDS             complement(39794..40357)
                     /gene="ahpC"
                     /locus_tag="ACHKYO_RS02925"
                     /old_locus_tag="pgaptmp_000585"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HGU4427481.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            40729..41475
                     /gene="dsbG"
                     /locus_tag="ACHKYO_RS02930"
                     /old_locus_tag="pgaptmp_000586"
     CDS             40729..41475
                     /gene="dsbG"
                     /locus_tag="ACHKYO_RS02930"
                     /old_locus_tag="pgaptmp_000586"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HGU4427482.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            41684..42586
                     /gene="ybdO"
                     /locus_tag="ACHKYO_RS02935"
                     /old_locus_tag="pgaptmp_000587"
     CDS             41684..42586
                     /gene="ybdO"
                     /locus_tag="ACHKYO_RS02935"
                     /old_locus_tag="pgaptmp_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4427483.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRSLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            42733..43953
                     /gene="ybdN"
                     /locus_tag="ACHKYO_RS02940"
                     /old_locus_tag="pgaptmp_000588"
     CDS             42733..43953
                     /gene="ybdN"
                     /locus_tag="ACHKYO_RS02940"
                     /old_locus_tag="pgaptmp_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU4427484.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARRMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            43926..44555
                     /gene="ybdM"
                     /locus_tag="ACHKYO_RS02945"
                     /old_locus_tag="pgaptmp_000589"
     CDS             43926..44555
                     /gene="ybdM"
                     /locus_tag="ACHKYO_RS02945"
                     /old_locus_tag="pgaptmp_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGU4427485.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(44556..45716)
                     /gene="ybdL"
                     /locus_tag="ACHKYO_RS02950"
                     /old_locus_tag="pgaptmp_000590"
     CDS             complement(44556..45716)
                     /gene="ybdL"
                     /locus_tag="ACHKYO_RS02950"
                     /old_locus_tag="pgaptmp_000590"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HGU4427486.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            45824..46912
                     /gene="hcxA"
                     /locus_tag="ACHKYO_RS02955"
                     /old_locus_tag="pgaptmp_000591"
     CDS             45824..46912
                     /gene="hcxA"
                     /locus_tag="ACHKYO_RS02955"
                     /old_locus_tag="pgaptmp_000591"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HGU4427487.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(46922..47119)
                     /gene="ybdD"
                     /locus_tag="ACHKYO_RS02960"
                     /old_locus_tag="pgaptmp_000592"
     CDS             complement(46922..47119)
                     /gene="ybdD"
                     /locus_tag="ACHKYO_RS02960"
                     /old_locus_tag="pgaptmp_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU4427488.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(47295..49400)
                     /gene="cstA"
                     /locus_tag="ACHKYO_RS02965"
                     /old_locus_tag="pgaptmp_000593"
     CDS             complement(47295..49400)
                     /gene="cstA"
                     /locus_tag="ACHKYO_RS02965"
                     /old_locus_tag="pgaptmp_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HGU4427489.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(49581..49994)
                     /gene="entH"
                     /locus_tag="ACHKYO_RS02970"
                     /old_locus_tag="pgaptmp_000594"
     CDS             complement(49581..49994)
                     /gene="entH"
                     /locus_tag="ACHKYO_RS02970"
                     /old_locus_tag="pgaptmp_000594"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HGU4427490.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(49997..50743)
                     /gene="entA"
                     /locus_tag="ACHKYO_RS02975"
                     /old_locus_tag="pgaptmp_000595"
     CDS             complement(49997..50743)
                     /gene="entA"
                     /locus_tag="ACHKYO_RS02975"
                     /old_locus_tag="pgaptmp_000595"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HGU4427491.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(50743..51600)
                     /gene="entB"
                     /locus_tag="ACHKYO_RS02980"
                     /old_locus_tag="pgaptmp_000596"
     CDS             complement(50743..51600)
                     /gene="entB"
                     /locus_tag="ACHKYO_RS02980"
                     /old_locus_tag="pgaptmp_000596"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HGU4427492.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(51614..53224)
                     /gene="entE"
                     /locus_tag="ACHKYO_RS02985"
                     /old_locus_tag="pgaptmp_000597"
     CDS             complement(51614..53224)
                     /gene="entE"
                     /locus_tag="ACHKYO_RS02985"
                     /old_locus_tag="pgaptmp_000597"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HGU4427493.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCLLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(53234..54409)
                     /gene="entC"
                     /locus_tag="ACHKYO_RS02990"
                     /old_locus_tag="pgaptmp_000598"
     CDS             complement(53234..54409)
                     /gene="entC"
                     /locus_tag="ACHKYO_RS02990"
                     /old_locus_tag="pgaptmp_000598"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HGU4427494.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            54784..55740
                     /gene="fepB"
                     /locus_tag="ACHKYO_RS02995"
                     /old_locus_tag="pgaptmp_000599"
     CDS             54784..55740
                     /gene="fepB"
                     /locus_tag="ACHKYO_RS02995"
                     /old_locus_tag="pgaptmp_000599"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU4427495.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAAQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(55744..56994)
                     /gene="entS"
                     /locus_tag="ACHKYO_RS03000"
                     /old_locus_tag="pgaptmp_000600"
     CDS             complement(55744..56994)
                     /gene="entS"
                     /locus_tag="ACHKYO_RS03000"
                     /old_locus_tag="pgaptmp_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HGU4427496.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            57105..58109
                     /gene="fepD"
                     /locus_tag="ACHKYO_RS03005"
                     /old_locus_tag="pgaptmp_000601"
     CDS             57105..58109
                     /gene="fepD"
                     /locus_tag="ACHKYO_RS03005"
                     /old_locus_tag="pgaptmp_000601"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HGU4427497.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            58106..59098
                     /gene="fepG"
                     /locus_tag="ACHKYO_RS03010"
                     /old_locus_tag="pgaptmp_000602"
     CDS             58106..59098
                     /gene="fepG"
                     /locus_tag="ACHKYO_RS03010"
                     /old_locus_tag="pgaptmp_000602"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HGU4427498.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHISRRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            59095..59910
                     /gene="fepC"
                     /locus_tag="ACHKYO_RS03015"
                     /old_locus_tag="pgaptmp_000603"
     CDS             59095..59910
                     /gene="fepC"
                     /locus_tag="ACHKYO_RS03015"
                     /old_locus_tag="pgaptmp_000603"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU4427499.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEKAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(59907..61040)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKYO_RS03020"
                     /old_locus_tag="pgaptmp_000604"
     CDS             complement(59907..61040)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKYO_RS03020"
                     /old_locus_tag="pgaptmp_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HGU4427500.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(61256..65137)
                     /gene="entF"
                     /locus_tag="ACHKYO_RS03025"
                     /old_locus_tag="pgaptmp_000605"
     CDS             complement(61256..65137)
                     /gene="entF"
                     /locus_tag="ACHKYO_RS03025"
                     /old_locus_tag="pgaptmp_000605"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HGU4427501.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINLAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLVQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGG
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISSEAFEKIGPIIRATLNR"
     gene            complement(65134..65352)
                     /gene="ybdZ"
                     /locus_tag="ACHKYO_RS03030"
                     /old_locus_tag="pgaptmp_000606"
     CDS             complement(65134..65352)
                     /gene="ybdZ"
                     /locus_tag="ACHKYO_RS03030"
                     /old_locus_tag="pgaptmp_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HGU4427502.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(65355..66557)
                     /gene="fes"
                     /locus_tag="ACHKYO_RS03035"
                     /old_locus_tag="pgaptmp_000607"
     CDS             complement(65355..66557)
                     /gene="fes"
                     /locus_tag="ACHKYO_RS03035"
                     /old_locus_tag="pgaptmp_000607"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HGU4427503.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            66800..69040
                     /gene="fepA"
                     /locus_tag="ACHKYO_RS03040"
                     /old_locus_tag="pgaptmp_000608"
     CDS             66800..69040
                     /gene="fepA"
                     /locus_tag="ACHKYO_RS03040"
                     /old_locus_tag="pgaptmp_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HGU4427504.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQS
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            69080..69178
                     /locus_tag="ACHKYO_RS03045"
                     /old_locus_tag="pgaptmp_000609"
                     /pseudo
     CDS             69080..69178
                     /locus_tag="ACHKYO_RS03045"
                     /old_locus_tag="pgaptmp_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            69215..69835
                     /gene="entD"
                     /locus_tag="ACHKYO_RS03050"
                     /old_locus_tag="pgaptmp_000610"
     CDS             69215..69835
                     /gene="entD"
                     /locus_tag="ACHKYO_RS03050"
                     /old_locus_tag="pgaptmp_000610"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HGU4427505.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(69957..70109)
                     /gene="hokE"
                     /locus_tag="ACHKYO_RS03055"
                     /old_locus_tag="pgaptmp_000611"
     CDS             complement(69957..70109)
                     /gene="hokE"
                     /locus_tag="ACHKYO_RS03055"
                     /old_locus_tag="pgaptmp_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU4427506.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            70078..70347
                     /locus_tag="ACHKYO_RS03060"
                     /old_locus_tag="pgaptmp_000612"
     CDS             70078..70347
                     /locus_tag="ACHKYO_RS03060"
                     /old_locus_tag="pgaptmp_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4427507.1"
                     /translation="MTATRAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPYLIN"
     gene            70551..71669
                     /gene="ybdK"
                     /locus_tag="ACHKYO_RS03065"
                     /old_locus_tag="pgaptmp_000613"
     CDS             70551..71669
                     /gene="ybdK"
                     /locus_tag="ACHKYO_RS03065"
                     /old_locus_tag="pgaptmp_000613"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HGU4427508.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFTSSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            71735..71983
                     /gene="ybdJ"
                     /locus_tag="ACHKYO_RS03070"
                     /old_locus_tag="pgaptmp_000614"
     CDS             71735..71983
                     /gene="ybdJ"
                     /locus_tag="ACHKYO_RS03070"
                     /old_locus_tag="pgaptmp_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HGU4427509.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            72048..72416
                     /gene="ybdF"
                     /locus_tag="ACHKYO_RS03075"
                     /old_locus_tag="pgaptmp_000615"
     CDS             72048..72416
                     /gene="ybdF"
                     /locus_tag="ACHKYO_RS03075"
                     /old_locus_tag="pgaptmp_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU4427510.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            72510..73163
                     /gene="nfsB"
                     /locus_tag="ACHKYO_RS03080"
                     /old_locus_tag="pgaptmp_000616"
     CDS             72510..73163
                     /gene="nfsB"
                     /locus_tag="ACHKYO_RS03080"
                     /old_locus_tag="pgaptmp_000616"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HGU4427511.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            73271..74518
                     /gene="ybdG"
                     /locus_tag="ACHKYO_RS03085"
                     /old_locus_tag="pgaptmp_000617"
     CDS             73271..74518
                     /gene="ybdG"
                     /locus_tag="ACHKYO_RS03085"
                     /old_locus_tag="pgaptmp_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HGU4427512.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(74586..75962)
                     /gene="pheP"
                     /locus_tag="ACHKYO_RS03090"
                     /old_locus_tag="pgaptmp_000618"
     CDS             complement(74586..75962)
                     /gene="pheP"
                     /locus_tag="ACHKYO_RS03090"
                     /old_locus_tag="pgaptmp_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HGU4427513.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(76064..79207)
                     /gene="cusA"
                     /locus_tag="ACHKYO_RS03095"
                     /old_locus_tag="pgaptmp_000619"
     CDS             complement(76064..79207)
                     /gene="cusA"
                     /locus_tag="ACHKYO_RS03095"
                     /old_locus_tag="pgaptmp_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HGU4427514.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(79219..80442)
                     /gene="cusB"
                     /locus_tag="ACHKYO_RS03100"
                     /old_locus_tag="pgaptmp_000620"
     CDS             complement(79219..80442)
                     /gene="cusB"
                     /locus_tag="ACHKYO_RS03100"
                     /old_locus_tag="pgaptmp_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HGU4427515.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNISKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(80458..80790)
                     /gene="cusF"
                     /locus_tag="ACHKYO_RS03105"
                     /old_locus_tag="pgaptmp_000621"
     CDS             complement(80458..80790)
                     /gene="cusF"
                     /locus_tag="ACHKYO_RS03105"
                     /old_locus_tag="pgaptmp_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HGU4427516.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGEV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(80823..>81161)
                     /locus_tag="ACHKYO_RS03110"
                     /old_locus_tag="pgaptmp_000622"
     CDS             complement(80823..>81161)
                     /locus_tag="ACHKYO_RS03110"
                     /old_locus_tag="pgaptmp_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HGU4427517.1"
                     /translation="GRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLND
                     QISAQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNE
                     ISLYTALGGG"
BASE COUNT        19897 a        21561 c        20284 g        19419 t
ORIGIN      
        1 ttcacatatg aataaaaaat taatgtatat attcgcaata tttatagttg cagcaattac
       61 ctgtattagc caacccaaga aaacaacgtt gcgtgataaa gccatggtga attatgcctt
      121 tgattattta agctcaccag gcagtctacc gttaaccacg gcagccacgg agctttccgc
      181 gattcatggt cactcaacat cgcaatatcg ccttggcgaa ttttatcttc atggtagcga
      241 cggtaaacca ctggattata cacaggcgag atactggtat gagcaatcag cagaacagga
      301 aaatccgcgc gcacaaagca aactggggtg gatctacctg aaaggcctga gagtcaaacc
      361 cgatacccgt aaagcaattc tctggtataa ggaagcagct gaacaagggt atgctcatgc
      421 tcaatatact ttgggtttga tctacagaaa tggcactggt attaatgtta accattatga
      481 atctcaaaaa tggttaaaac tggccgccaa acaacattac aaaaatgcgg aaagattact
      541 tgccggactt cccgcacatt aacgtcgact gtcacccgaa gagatggagt attttcccta
      601 tcaaaccggc aaagataaaa atgtagaaaa taattttgat gacgcccaga cagcccagcc
      661 cgccatcgtc aacgggcatt ggctgcggtt gcggctccgg ctcgacggtt tcctgaccgg
      721 taaaattcgc cgcctgaatt aataatcgac gaataattaa tgcttccagt ggacaacgat
      781 cttctgcatc aaggcggctc acaatcggca ttaatgattc ttcggttggt tggctggcct
      841 gcactaattg tttggcaact aaatataatg gccataaggg atgttctgta tcacgcgtga
      901 gtgagatccg cggcttacgt cctttgtgca aaaccatctc gaccagtgaa cgccccagag
      961 cagagagttt ttcatcgctg agtatttcgt ataaatgaaa acgcgtacgg gcgctgtcgc
     1021 cggggacata aagtagcggt tttcccttat cgttaaccag atcacgcgct tcaatcatca
     1081 tcaccactaa cgggtaataa tcgacccgat gctgacggca ccattgcaac attaaagcat
     1141 ccgcctgggt ggagggatac tcgcgccagt agtcacaaag ttctgccagc aagctttcgc
     1201 cttctccaca atagacgcgt tgcgcgtatt cataataacg cgcatcttca ttatcaggtt
     1261 cagcttcccg ccacgaccgg gcgtaatcca gaagttcggg gatatccagg cgacctgcgc
     1321 tataccagcg gagtaaattt ctgtgcagtt ttgcatcatc ggggatgatc actgcgcgat
     1381 gttgcgctaa atacaatgtc agccattctg gatgcgatga ccaaatccga gcgaacattt
     1441 ggtaaaaatc gatggtgtca ttttgcacgg caacgggcag atgcagaatg gtctggaagt
     1501 taaaaacatt ccccttcgca atggcatata aaaaggattc cagttcttct tcgtctattt
     1561 ccccgacgtt ttcctcctgc aaccaccgca atctttctgc taataacacc acgcactcga
     1621 aggataaatg ggcagtgttc atggcgattg tgcacagcga ccagcgtaat tcatcaatat
     1681 cttccatcga gcaataattt aattgctgaa tgaatcgctg ccaggcggag ggcagaaaac
     1741 gttcgctatc agaggcaagt aacgcgcgaa aggcgagcag aatttcatgt tctgctacct
     1801 catattcttc tggttgccaa acagatttag caggcgactg cgcaatccgt agcgcctcct
     1861 catacgcctg acgaagttgt ttaaaaccct gcggatcggt ttctggatga aaggacggta
     1921 acagcgccag ataagcgcgg cggataatat cgacgtcagt cgtttcctct atttcgagga
     1981 tcttccagca atttttcata ggtcgctcat atccttaagc gtttccgggc atgcagaata
     2041 gtaagccaat ggttgccagg acaaggtagg gtaatacgca ttatcactgg caaataattg
     2101 ataaaacata tcattgccgg aatcgtcgta ttctttacgc gctaagaatt cacgtaataa
     2161 ctgcagatta tcatcttcgg gagaatgcca atattgccga ccaaaggtat aaccggcaaa
     2221 gtattgccgc cagttacgat aataacgtag agcccgtaga tgaatgcgcg actggatcca
     2281 tagactttct tcttcgctca accagttatt aagtacgccc attcggctta aaaaacccat
     2341 ccggacataa tcccaggctt taattcctcc ctcaccacag cattcggcgg tgctggcgac
     2401 aaattgcgca taagcctgac cttgctcatt tagttcagca agatagttac gccattcaca
     2461 tggagaataa cgaaaccagc ggtgataaaa ccctgccaac cgagcggcat gaccgttctc
     2521 gatcattcgg tgaattgtgc gcagcaggtc atcacggtca tcaattcccc atgaggaaga
     2581 aaggtcaaaa aagcatgtac ctaaatacat tttgggtgat gaatgcgaag gtaaacagca
     2641 ttcattgcgt atttccatcg gagtggaaag tgcaaacaat attttttgcg actccatgtc
     2701 catataattt atccttaaat attcattaaa tgaatattag ccgcattgcg aggctcgttt
     2761 atttatactt ttctgacgcg acataatacg cataaaataa ggcttacgca aaaatggaac
     2821 acataaaaat ataatatgtt ttatctgcaa ccataaggaa atgataatga ttttcacgtc
     2881 aagttgctgc gataatttat caatagatga gattatcgaa cgtgctgaaa aaggcgattg
     2941 cgaggctcaa tatattgttg ggttttatta taatcgcgat agcgcaattg attctccgga
     3001 cgacgaaaaa gccttttact ggctgaagct ggccgccgag caaggtcatt gtgaagcaca
     3061 atattcctta ggacaaaagt atactgagga taaaagccgt cataaagata atgagcatgc
     3121 catcttttgg ctgaaaaaag ctgccctaca aggccatact ttcgcttcca acgcccttgg
     3181 ctggatactg gatcgtggag aagaccccaa ttataaagaa gcggttgtct ggtatcagat
     3241 agccgcggag agcggaatgt cttatgcgca aaataatctt gggtggatgt acagaaatgg
     3301 caacggagtc gcaaaagact atgcgctggc atttttctgg tacaaacaag ctgcattaca
     3361 aggccatagt gacgcgcaaa acaatctggc cgatctttat gaagacggaa aaggcgttgc
     3421 tcaaaacgag acactcgccg cattctggta tttgaaaagc gcacagcagg gtaatcggca
     3481 tgctcagttt caaattgcat gggattataa cgctggcgaa ggggtggacc aggactataa
     3541 gcaagcgatg tactggtatc tgaaggctgc cgctcagggg agcgtcggcg cttacgtcaa
     3601 catcggttat atgtataaac acggacaagg cgttgagaaa gattatcagg ctgcctttga
     3661 atggtttacg aaagccgctg aatgcaatga cgccactgcc tggtataacc tggccattat
     3721 gtatcactac ggagaaggaa gacctgtcga tctccgacag gctctcgacc tgtatcgtaa
     3781 agttcagtca tccggaacca gagatgtcag tcaggaaatt cgtgagactg aagatttact
     3841 gtagagataa aaacaagggc cggggaattt cattcggccc atattctctt tattgctgtg
     3901 cgaaatcaga ctattacggc tcaaacgggc gtctctggaa ctggtcatga ccacatttcg
     3961 ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg gaagtgacat ttctcgcaga
     4021 ccagatttcc cagcccgacc acttctccgc tgtgataaac cccatgatga ttgaggtcct
     4081 ggaaaacttc gcgccattca agctgcgttt tatcggtgat atctgccagc tcctgccata
     4141 agctttcttt aatcacccgc ataaagacgc tgtcagattc ttctttcagg ctctcttcat
     4201 agctcatggc gaactcttcc aggtcacgtc ttacagctcg cgtcagctca tcgacctcgg
     4261 ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc ctgttccacc agtgcgtcga
     4321 tatcacgttc gccattgcgc aggcgttcgc tcagtgacgc gaccagttca cggtaatatt
     4381 gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac tgactataat aatagacgtt
     4441 ctttgttcct tgctttgcta atacgcccac aaactctgta aattaatgta tgaataatcc
     4501 gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg acgcggacgt ttgggctatg
     4561 ctatgcggat ctgaaaaacc acctcaacgc tacatttgta gccgtattga aaacaggacc
     4621 actggctgcc atgcaagagc aataccgccc ggaagagata gaatccaaag tacagcttca
     4681 ttgggatgag aagcgcacat ttgaagtaac cgaagacgag agcaaagaga agtattactg
     4741 cctgtctatg cttccctatc cttctggtcg actacacatg ggccacgtac gtaactacac
     4801 catcggtgac gtgatcgccc gctaccagcg tatgctgggc aaaaacgtcc tgcagccgat
     4861 cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg gtgaaaaaca acaccgctcc
     4921 ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac cagctcaaaa tgctgggctt
     4981 tggttatgac tggagccgcg agctggcaac ctgtacgccg gaatactacc gttgggaaca
     5041 gaaattcttc accgagctgt ataaaaaagg cctggtatat aagaagactt ctgcggtcaa
     5101 ctggtgcccg aacgaccaga ccgtactggc gaacgaacaa gttatcgacg gctgctgctg
     5161 gcgctgcgat accaaagttg aacgtaaaga gatcccgcag tggtttatca aaatcactgc
     5221 ttacgctgac gagctgctca acgatctgga taaactggat cactggccag acaccgttaa
     5281 aaccatgcag cgtaactgga tcggtcgttc cgaaggcgtg gagatcacct tcaacgttaa
     5341 cgactatgac aacacgctga ccgtttacac tacccgcccg gacaccttta tgggttgtac
     5401 ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa gcggcggaaa ataatcctga
     5461 actggcggcc tttattgacg aatgccgtaa caccaaagtt gccgaagctg aaatggcgac
     5521 gatggagaaa aaaggcgtcg atactggctt taaagctgtt cacccattaa cgggcgaaga
     5581 aattcccgtt tgggcagcaa acttcgtatt gatggagtac ggcacgggcg cagttatggc
     5641 ggttccgggt cacgaccagc gcgactacga gtttgcctct aaatacggcc tgaacatcaa
     5701 accagttatc ctggctgctg acggctctga gccagatctt tctcagcaag ccctgactga
     5761 aaaaggcgtg ctgttcaact ctggcgaatt caacggtctt gaccatgaag cggccttcaa
     5821 cgccatcgcc gataaactga ctgcgatggg cgttggcgag cgtaaagtga actaccgcct
     5881 gcgcgactgg ggtgtttctc gtcagcgtta ctggggcgcg ccgattccga tggtgacgct
     5941 ggaagacggt accgtaatgc cgaccccgga cgaccagctg ccggtgatcc tgccggaaga
     6001 tgtggtgatg gacggcatta ccagcccgat taaagcagat ccggagtggg caaaaactac
     6061 cgttaacggt atgccagcgc tgcgtgaaac cgacactttc gacaccttta tggagtcctc
     6121 ctggtactat gcgcgctaca cttgcccaga gtacaaagaa ggtatgctgg attccgaagc
     6181 ggctaactac tggctgccgg tggatatcta cattggtggt atcgaacacg ccattatgca
     6241 cctgctctac ttccgcttct tccacaaact gatgcgtgat gcaggcatgg tgaactctga
     6301 cgaaccagcg aaacagttgc tgtgtcaggg tatggtgctg gcagatgcct tctactatgt
     6361 tggcgaaaac ggcgaacgta actgggtttc cccggttgat gctatcgttg aacgtgacga
     6421 gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat gaactggttt ataccggcat
     6481 gagcaaaatg tccaagtcga agaacaacgg tatcgacccg caggtgatgg ttgaacgtta
     6541 cggcgcggac accgttcgtc tgtttatgat gtttgcttct ccggctgata tgactctcga
     6601 atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg aaacgtgtct ggaaactggt
     6661 ttacgagcac acagcaaaag gtgatgttgc ggcactgaac gttgatgcgc tgactgaaga
     6721 tcagaaagcg ctgcgtcgcg atgtgcataa aacgatcgct aaagtgaccg atgatatcgg
     6781 ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg gagctgatga acaaactggc
     6841 gaaagcacca accgatggcg agcaggatcg cgctctgatg caggaagcgc tgctggccgt
     6901 tgtccgtatg cttaacccgt tcaccccgca catctgcttc acgctgtggc aggaactgaa
     6961 aggcgaaggc gatatcgaca acgcgccgtg gccggttgct gacgaaaaag cgatggtgga
     7021 agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc cgtgccaaaa tcaccgttcc
     7081 ggtggacgca acggaagaac aggttcgcga acgtgctggc caggaacatc tggtagcaaa
     7141 atatcttgat ggcgttaccg tgcgtaaagt gatttacgta ccaggtaaac tcctcaatct
     7201 ggtcgttggc taagcgcggg aggaagcgtg cgatatctgg caacattgtt gttatctctg
     7261 gcggtgttaa tcaccgccgg gtgtggctgg catctgcgtg ataccacgca ggttccttcc
     7321 actatgaagg tcatgatcct ggactcaggc gatccgaacg ggccattaag ccgtgcggtg
     7381 cgtaaccagt tacgtctgaa tggtgtcgag ttgcttgata aagaaaccac gcgtaaggac
     7441 gttccatccc tgcgtttggg taaagtgagc atcgcgaaag ataccgcatc ggtattccgt
     7501 aacggtcaaa cagcagagta tcagatgatc atgacggtga atgcgaccgt gttgatcccc
     7561 ggccgtgata tctacccgat tagtgccaaa gtcttccgtt cgttctttga taacccgcaa
     7621 atggcgttag cgaaagataa cgaacaagac atgatcgtaa aagagatgta cgaccgtgct
     7681 gccgaacagc tgattcgtaa gctgccaagc atccgtgctg cggatattcg ttccgacgaa
     7741 gaacagacgt cgaccacaac ggatactccg gcaacgcctg cacgcgtctc caccacgctg
     7801 ggtaactgat gattcggttg tacccggaac aactccgcgc gcagctcaat gaagggctgc
     7861 gcgcggcgta tcttttactt ggtaacgatc ctctgttatt gcaggaaagc caggacgctg
     7921 ttcgtcaggt agctgcggca caaggattcg aagaacacca cactttttcc attgatccca
     7981 acactgactg gaatgcgatc ttttcgttat gccaggctat gagtctgttt gccagtcgac
     8041 aaacgctatt gctgttgtta ccagaaaacg gaccgaatgc ggcgatcaat gaacaacttc
     8101 tcacactcac cggacttctg catgacgacc tgctgttgat cgtccgcggt aataaattaa
     8161 gcaaagcgca agaaaatgcc gcctggttta ctgctcttgc gaatcgcagc gtgcaggtga
     8221 cctgtcagac accggagcag gctcagcttc cccgctgggt tgctgcgcgc gcaaaacagc
     8281 tcaacttaga actggatgac gctgcaaatc aggtgctctg ctactgttat gaaggtaacc
     8341 tgctggcgct ggctcaggca ctggagcgtt tatcgctgct ctggccagac ggcaaattga
     8401 cattaccgcg cgttgaacag gcggtgaatg atgccgcgca tttcacccct tttcattggg
     8461 ttgatgcttt gttgatggga aaaagtaagc gcgcgttgca tattcttcag caactgcgtc
     8521 tggaaggcag cgaaccggtt attttgttgc gcacattaca acgtgaactg ttgttactgg
     8581 ttaacctgaa acgccagtct gcccatacgc cactgcgtgc gttgtttgat aagcatcggg
     8641 tatggcagaa ccgccggggc atgatgggcg aggcgttaaa tcgcttaagt cagacgcagt
     8701 tacgtcaggc cgtgcaactc ctgacacgaa cggaactcac cctcaaacaa gattacggtc
     8761 agtcagtgtg ggcagagctg gaagggttat ctcttctgtt gtgccataaa cccctggcgg
     8821 acgtatttat cgacggttga tatgaaatct ttacaggctc tgtttggcgg cacctttgat
     8881 ccggtgcact atggtcatct taaacccgtg gaaacgctgg cgaatttgat tggtctgacg
     8941 cgggtcacaa tcatccctaa taatgttcct ccgcatcgtc cccagccgga agcgaacagc
     9001 gtgcagcgta aacacatgct tgaactggcg attgccgaca agccattatt tactcttgat
     9061 gaacgcgagc taaagcgcaa tgccccctct tacactgcgc aaacactgaa agagtggcgg
     9121 caggaacaag ggccggacgt gccgctggcg tttattattg gtcaggattc attgctgacc
     9181 tttccgacct ggtacgaata cgaaacgata ctcgacaatg cacatttgat cgtctgtcgg
     9241 cgtccaggtt acccacttga aatggcgcaa ccgcaatacc agcaatggct ggaagatcat
     9301 ttgacacata acccggaaga tcttcacctt cagcctgccg gtaaaattta tctggctgaa
     9361 acgccgtggt ttaacatctc ggcgaccatc atccgcgaac gtttgcaaaa cggtgaatcg
     9421 tgtgaggatt tattgccgga accggtactg acttacatta accaacaagg cttgtatcgc
     9481 tgatacctgt cgttacggag ggcatgatgc gactgtggtt aattcgtcat ggtgaaacgc
     9541 aagcgaatat cgatggtcta tacagcggtc atgcgcccac ccccctgacc gcgcgcggta
     9601 ttgagcaagc gcaaaatctg catacgctgc tacatggtgt ttcctttgat ctggttttat
     9661 gcagtgaact ggaacgggca cagcataccg cgcgactggt tctcagtgac cgccagctcc
     9721 ccgtgcaaat catccctgaa ctcaacgaaa tgttttttgg cgactgggag atgcgacatc
     9781 atcgcgacct catgcaagaa gatgccgaaa actatagcgc gtggtgcaat gactggcagc
     9841 atgcaatccc cacgaacggt gaaggatttc aggcattttc gcaacgtgtg gaacgcttta
     9901 tcgcaaggct tagtgaattt cagcactatc agaatatttt agtcgtcagc catcagggtg
     9961 tactgagtct gttaatcgcc cgtttaattg gcatgcctgc cgaggctatg tggcattttc
    10021 gcgttgacca ggggtgttgg agcgccattg atatcaacca aaaattcgcg acgctacgcg
    10081 tcctcaatag ccgtgccatc ggggtcgaga atgcatgact tttctgtttt tttacgggta
    10141 agccgcaacg accattgaca gacccgggca ggctgatatt ctccgcagcc agactttttt
    10201 cgccagacac gactttgtag aaattgtttt acaaaaatgg cgatgcaatc tgcggcgcgg
    10261 ggtgggatga tagcccactt tcgaaagccg attcggcgac aattgtcccg aaatcgcctc
    10321 tggttcaggt atactgacag accattttta tctatttgat tcacccaggg ggaaaacttg
    10381 cagggtaaag cactccagga ttttgttatc gacaaaattg atgacctcaa aggtcaggac
    10441 atcatcgcct tagacgttca aggcaaatcc agcatcaccg actgcatgat catctgtacg
    10501 ggtacgtcca gccgtcatgt tatgtccatt gctgaccacg ttgtgcagga gtctcgcgca
    10561 gcgggcctgt taccgctcgg cgtagaaggt gaaaacagcg ccgactggat tgtcgtggat
    10621 ttgggcgatg tgattgtcca tgtcatgcag gaagagagcc gtcgcctgta tgaactggaa
    10681 aaactctgga gttaatgcgt gaagctgcaa cttgtcgccg tgggaacgaa aatgccggac
    10741 tgggtacaaa ccggttttac cgagtacctg cgtcgttttc cgaaagatat gcccttcgag
    10801 ctgattgaaa ttccggccgg aaaacgcggc aagaatgcgg acatcaagcg catactcgac
    10861 aaagagggtg agcagatgtt ggcggccgca ggcaaaaacc gcattgtcac cctcgatatt
    10921 ccaggcaagc cctgggatac gccgcagtta gccgctgagc tggaacgctg gaagctggat
    10981 ggtcgcgacg tcagtctact gattggcggg cctgaagggt tgtcgcctgc ctgtaaagcg
    11041 gcggcggagc agagctggtc gctgtcgacg cttaccctcc cccatccgct ggttcgcgtg
    11101 ctggtcgcag agagtctgta ccgggcgtgg agcatcacca ccaaccatcc ttatcaccgt
    11161 gagtgataag ggagctttga gtagaaaacg cagcggatga aactacagaa ctcttttcgc
    11221 gactatacgg cagagtccgc gctgtttgtg cgccgggcgc tggtcgcctt tttggggatt
    11281 ttgctgctga ccggcgtgct tatcgccaac ctgtataatc tgcaaattgt tcgctttacc
    11341 gattaccaga cccgctctaa tgaaaaccgc attaagctgg tgcctatcgc gcccagccgc
    11401 ggcattatct acgaccgtaa cggtatccct ctggcactca accgcactat ctaccagata
    11461 gaaatgatgc cggagaaagt cgataacgtg cagcaaacgc tggacgcttt gcgcagcgtg
    11521 gttgatctga ccgatgacga tattgctgca ttccgaaaag agcgcgcacg ttcacaccgt
    11581 ttcacctcta ttcccgtgaa aactaacctg accgaagtac aagtagctcg ctttgccgtc
    11641 aatcagtacc gttttccggg tgtcgaagtt aaaggctata aacgtcgtta ctatccttac
    11701 ggttcggcgt tgacccacgt catcggctat gtgtcgaaaa tcaacgataa agacgtcgaa
    11761 cgcctgaata atgacggcaa actggccaac tatgcggcaa cgcatgatat cggtaagctg
    11821 ggcattgagc gttactatga agacgtgctg cacggtcaga ccggttatga agaggttgaa
    11881 gttaacaacc gtgggcgtgt tatccgccag ttaaaagaag taccaccgca agccggacac
    11941 gatatttacc tgacgctgga tctcaaactc cagcaatata ttgaaacgct gctggcgggt
    12001 agccgcgcag ctgtggtagt caccgatccg cgtacaggtg gggtgctggc actggtttcc
    12061 acgcctagtt atgacccaaa cctgtttgtt gacggtatct ccagcaaaga ttattccgcc
    12121 ttgctgaacg acccgaatac accgctggtg aaccgcgcca cacagggggt ttatcctcct
    12181 gcgtctacag ttaaacccta tgtggcggtt tcggcattaa gcgccggggt gatcacgcgc
    12241 aatacgacgc tgtttgaccc aggctggtgg caactaccag ggtcggaaaa acgttatcgt
    12301 gactggaaaa aatggggcca cgggcgtctg aatgtcacaa gatcgctgga agaatctgcg
    12361 gataccttct tctatcaggt ggcctacgat atggggatcg atcgcctctc cgaatggatg
    12421 ggtaaattcg gttatggtca ttacaccggt atcgacctgg cggaagaacg ttccggcaac
    12481 atgcctaccc gcgaatggaa acagaaacgc tttaaaaaac cgtggtatca gggtgacacc
    12541 attccggttg gtatcggcca gggttactgg acagcgaccc caatccagat gagtaaggca
    12601 ctgatgatcc tgattaatga cggtatcgtg aaggttccgc atttgctgat gagcaccgcc
    12661 gaagacggca aacaggtgcc atgggtacaa ccgcatgaac cgcccgtcgg cgatattcat
    12721 tccggttact gggagctggc gaaagacggt atgtacggtg ttgctaaccg ccctaacggt
    12781 acggcgcata aatactttgc cagcgcaccg tacaaaattg cggcgaaatc tggtaccgct
    12841 caggtcttcg gtctgaaagc gaacgaaacc tataatgcgc acaaaattgc cgagcgttta
    12901 cgtgaccaca aactgatgac cgcctttgcg ccatacaaca atccgcaagt ggctgtcgcc
    12961 atgattctgg agaacggcgg cgcgggtccg gcggttggta cgctgatgcg ccagatcctc
    13021 gaccacatta tgctgggtga taacaatacc gatctgcctg cggaaaatcc tgcggttgcc
    13081 gcagcggagg accattaatc atgacggata atccgaataa aaaaacattc tgggataaag
    13141 tccatctcga tcccacaatg ctgctgatct tactggcatt gctggtttac agcgccctgg
    13201 ttatctggag cgccagcggt caggatattg gcatgatgga gcgtaaaata ggccaaatcg
    13261 cgatgggtct ggtcatcatg gtggtgatgg cgcaaattcc tccacgcgtt tatgaaggct
    13321 gggcccccta tctctatatc atctgtatta ttttgctggt ggcggtagat gctttcggtg
    13381 ccatctctaa aggcgctcaa cgctggctgg acctcggtat tgttcgtttt cagccgtcgg
    13441 aaattgccaa aatagccgta ccactgatgg ttgcgcgctt tatcaaccgc gacgtttgcc
    13501 cgccatcgtt gaagaacact ggcatcgcgc tggtgctgat atttatgccc acgctgctgg
    13561 tggctgcaca gcctgacctg ggaacatcaa tcctcgttgc gctttccggt ctgtttgtac
    13621 tgttcctctc tggccttagc tggcgtctga ttggcgtcgc agtagtgctg gtggcggcgt
    13681 tcattcctat tctgtggttc ttcctgatgc atgattacca gcgccagcgc gtaatgatgc
    13741 tcctggaccc ggaatcagac ccactcggcg cgggctatca cattattcag tctaaaattg
    13801 ctattggctc cggcggatta cgcggcaaag gctggctgca tggcactcag tcacaacttg
    13861 aatttctacc cgaacgccat accgacttta tcttcgcggt actggcggaa gagctgggat
    13921 tagtgggcat tctgattctg ctcgctctct acattctgct gatcatgcgc gggctgtgga
    13981 tagccgccag agcgcaaacc acctttggtc gcgtcatggc tggtggctta atgctgatat
    14041 tattcgttta tgtcttcgta aatattggta tggtaagcgg tattctgccg gttgtagggg
    14101 ttccgctccc actggtcagt tatggaggat cggcgctaat tgtgctgatg gctgggttcg
    14161 ggattgtaat gtcaatccac acccacagga aaatgttgtc gaaaagcgtg taagaggtgc
    14221 gcaatgcgta agcagtggct cgggatctgc atcgcggcag gaatgctcgc ggcatgtaca
    14281 agcgatgatg gtcagcaaca gacggtaagt gtaccgcagc ctgcggtatg taacggcccc
    14341 atagttgaaa ttagcggggc ggacccgcgt ttcgaaccac tgaacgcgac ggcaaatcag
    14401 gattaccagc gcgacggtaa aagctacaaa atcgtgcagg atccgtctcg atttagccag
    14461 gcgggactgg cggcaatcta tgatgccgaa ccaggcagta acctgacggc ctctggcgaa
    14521 gctttcgatc cgacacagct gacggcggcc catccaacgc ttccgatccc cagctacgcc
    14581 agaatcacta acctggctaa cgggcgaatg atcgtggtgc gcattaatga tcgcggccct
    14641 tacggcaacg accgcgttat ttcgctttct cgcgcggcag ctgaccgtct taacacgtca
    14701 aacaacacca aagttcgtat cgatccgatt attgtcgccc aggatggttc gctttctggt
    14761 cctggtatgg cttgtaccac agtcgccaaa cagacttacg ccctgcctgc acctcccgat
    14821 ttaagcggtg gcgcgggaac aagttcagtg tctggcccgc agggtgacat tcttccggtc
    14881 agtaattcga cgctaaaaag cgaagatccg atcggcgcgc cggtaaccag tagcggtttc
    14941 ctcggcgctc caacgacctt agcgcctggt gtactggaag gcagcgaacc gacgcctgcg
    15001 ccacagcccg ttgttacagc tccgtcgaca acgcctgcaa cctcgcctgc aatggtgaca
    15061 ccgcaagccg cctcgcaaag cgccagtggc aactttatgg tgcaagtcgg ggccgtaagc
    15121 gatcaggctc gtgcgcaaca gtaccaacag caactgggac agaagttcgg cgtccccggt
    15181 cgcgtaactc aaaatggcgc ggtctggcgg atccagcttg gcccattcgc cagcaaagcc
    15241 gaagccagta ccttgcagca acgtttgcaa accgaagccc aattacagtc atttattact
    15301 accgcgcagt agcgtaaagc aggcatctga tgtgtcaatt tctgtaaatg acgttaacaa
    15361 agtcacgcgg aaagtcagat gcctgccggt agtgcatttg ctatagtagg gcactttttt
    15421 aattccatca cggatgtcgt agttcagacc atgaatacca ttttttccgc tcgtatcatg
    15481 aagcgcctgg cgctcaccac ggctctttgc acagccttta tctctgctgc acatgccgat
    15541 gacctgaata tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc
    15601 ctgattgact ataactccgg caaagtgctc gccgaacaga acgcagatgt ccgccgcgat
    15661 cctgccagcc tgaccaaaat gatgaccagt tacgttatcg gccaggcaat gaaagccggt
    15721 aaatttaaag aaactgattt agtcactatc ggcaacgacg catgggccac cggtaacccg
    15781 gtgtttaaag gttcttcgct gatgttcctc aaaccgggca tgcaggttcc ggtttctcag
    15841 ctgatccgcg gtattaacct gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt
    15901 gccgctggta gccaggacgc ttttgttggc ttgatgaaca gctacgttaa cgcactgggc
    15961 ctgaaaaata cccacttcca gacggtacat ggtctggatg ctgatggtca gtacagctcc
    16021 gcgcgagata tggcgctgat cggccaggca ttgatccgtg acgtaccgaa tgaatactcg
    16081 atctataaag aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg taacggcctg
    16141 ttatgggata acagcctgaa tgtcgacggc atcaaaaccg gacacactga caaagcaggt
    16201 tacaaccttg ttgcttctgc gactgaaggc cagatgcgct tgatctctgc ggtgatgggc
    16261 ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac tgctaacctg gggcttccgt
    16321 ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt tcgcctctga accggtttgg
    16381 tttggtgatt ctgatcgcgc ttcgttaggt gttgataaag acgtgtacct gaccattcca
    16441 cgtggtcgca tgaaagatct gaaagccagc tatgtgctga acagcagtga attgcatgcg
    16501 ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc agcttgatgg caaaacgatc
    16561 gagcaacgcc cgctggttgt gttgcaagaa atcccggaag gtaacttctt cggcaaaatc
    16621 attgattaca ttaaattaat gttccatcac tggtttggtt aaaaattgaa cacttgaaag
    16681 tgtaatttcc gtccccatat actaagcatc agtaaaaaaa ctcccgcctt ctggcgggag
    16741 ttgctattta attacgttac gccggagctg acatgaaaac caaacttaac gaactgcttg
    16801 aattccctac tccttttact tacaaagtta tggggcaggc gttacctgag ctggttgatc
    16861 aggtggttga agtggtacag cgccatgcgc caggtgacta caccccaacg gtaaaaccaa
    16921 gcagcaaagg caactaccac tcggtatcta tcactatcaa cgccactcat atcgagcagg
    16981 ttgaaacact gtatgaagaa ctgggcaaaa tcgatattgt ccgcatggtt ctgtaactcg
    17041 cttctccgtt acccggctcc tggtcgggta actcccctct cctcgctgtg atatactttt
    17101 cccccacttt tactcattct ccacggagat gccgttttgt atcaggataa aattcttgtc
    17161 cgccagctcg gtcttcagcc ttacgagcca atctcccagg ctatgcatga attcaccgat
    17221 acccgcgatg atagtaccct tgatgaaatc tggctggtcg agcactatcc ggtattcacc
    17281 caaggtcagg caggaaaagc ggagcacatt ttaatgccgg gtgatattcc ggtgatccag
    17341 agcgatcgcg gtgggcaggt gacttatcac gggccgggac aacaggtgat gtatgtgttg
    17401 cttaacctga aacgccgtaa actcggtgtg cgtgaactgg tgaccttgct tgagcaaaca
    17461 gtggtgaata ccctggctga actgggtata gaagcgcatc ctcgggctga cgcgcctggt
    17521 gtctatgtcg gggaaaagaa aatttgctca ctgggtttac gaattcgacg cggttgttca
    17581 ttccacggtc tggcattaaa cgtcaatatg gatctttcac catttttacg tattaatcct
    17641 tgtgggtatg ccggaatgga aatggctaaa atatcacaat ggaaacccga agcgacgact
    17701 aataatattg ctccacgttt actggaaaat attttagcgc tactaaacaa tccggacttc
    17761 gaatatatta ccgcttaatt ccatacatca atggcccaat ttacactggg tcattactca
    17821 taatacctgc taatgcttta catctccttc gcgtttgtat attttcttta tcacttgcag
    17881 aaatgcggtg ccacgccttg ctccgtaact caccccattg attaacctat tgaaatagtt
    17941 gattatggtg agcaaaagcg ttccgttcca attagacata agattattgt aattttttaa
    18001 atattatttt tccatgaata aaattggagt aagtgcgtgg atagtaataa tcaaattgaa
    18061 ccctgtttaa gcagaaagtc gtcagaaggt aaaccacaaa tatttacaac cttacgaaat
    18121 attgatctta accttctgac tatttttgaa gctgtatatg tacataaagg gatcgttaat
    18181 gcagcgaaag tgcttaatct gaccccctcg gcaatcagtc agtctattca gaaactgcgc
    18241 gttatattcc ctgacccatt gtttattcgc aaaggccagg gtgtcactcc taccgcattt
    18301 gcgatgcatc tacatgagta tatcagtcag ggccttgagt ccattcttgg cgcgctggat
    18361 atcgaaggaa gctatgataa gcaacgaacg ataactattg cgaccactcc ctcggtcgga
    18421 gccctggtcc ttcctgtcat ctatcgggcg attaaaactc actatccgca gcttttactg
    18481 cgcaacccgc ccaccagcga cgcggaaaac caactcagtc agtttcaaac cgatctcatc
    18541 atcgataaca tgttttgcac caatcgtacg gtgcaacatc atgttctgtt caccgacaat
    18601 atggtgttaa tttgccgcga gggaaatcca ctactctctt tagaagatga cagagagact
    18661 atcgacaacg ctgcgcatgt acttctgtta ccggaagagc aaaatttcag cggtctgcgg
    18721 cagagagttc aagaaatgtt tccggaccgg caaatcaatt tcaccagcta caacattttg
    18781 acaatcgctg cactggttgc caacagtgac atgttagcga ttattcccag ccgtttttat
    18841 aacctgttta gccgctgctg gccgctggaa aaactgcctt ttccgtcctt aaatgaggag
    18901 caaatagatt tctctatcca ctacaacaaa ttcagcctgc gtgatcccat cctgcacgga
    18961 gtaatagatg ttatccgtaa tgcattttga aaaagtattg ttacagccag atcacagcgc
    19021 agaacgataa aatggcacaa tccaacaaca attttacatt ttcgcgaccg ctttggctgc
    19081 tttcaggtcc gtttcaatga tatactgcca gtcgttaatt caaaaatagt tgataattac
    19141 aacaatctat tgaattgaaa cgctttcctt cgtaattcgc aactggaaca cgcacgctat
    19201 gagtaaaccc attgtgatgg aacgcggtgt taaataccgc gatgccgata agatggccct
    19261 tatcccggtt aaaaacgtgg caacagagcg cgaagccctg ctgcgcaagc cggaatggat
    19321 gaaaatcaag cttccagcgg actctacacg tatccagggc atcaaagccg caatgcgcaa
    19381 aaatggcctg cattctgtct gcgaggaagc ctcctgccct aacctggcgg aatgcttcaa
    19441 ccacggcaca gcaacgttta tgatcctcgg cgctatttgt acccgccgtt gtccgttctg
    19501 tgatgttgcc cacggtcgcc cggtagctcc tgatgccaat gaaccagtga aactggcgca
    19561 gaccattgcc gatatggcgc tgcgttatgt ggttatcacc tccgttgacc gtgatgacct
    19621 gcgcgatggc ggtgcccagc actttgcgga ttgcattact gccattcggg aaaaaagccc
    19681 gcaaatcaaa attgaaactc tggtgccgga tttccgtggt cgtatggatc gtgctctgga
    19741 tattctgact gcaacgccac cagatgtgtt caaccataac ctggaaaacg taccgcgtat
    19801 ttaccgtcag gtacggcctg gtgcagatta caactggtcg ctgaagctgc tggaacgctt
    19861 taaagaagcg catccggaaa tcccgaccaa gtctggtctg atggtgggac tgggtgaaac
    19921 caatgaagaa attattgagg taatgcgcga cctgcgccgt catggtgtga cgatgttaac
    19981 gctggggcaa tatttgcagc caagccgcca tcacctgccg gttcaacgtt acgttagccc
    20041 ggatgagttc gacgaaatga aagccgaagc gctggcgatg ggctttaccc atgctgcatg
    20101 cggtccgttt gtccgctctt cttaccacgc cgatttgcag gcgaaaggga tggaagttaa
    20161 gtaatcctgt aataattact tacatctgtc tgataaaaaa cccgcttttt gaagcgggtt
    20221 ttttgtatcg aacaagatat tgagggagcg tcctgctcgc cacgtcactc tttatgagag
    20281 agcttttcag cctgaagatc aacgtctgcg ccttttttcg ccgcagcatc gtcatcattc
    20341 atcgccttct tgaacccttt aatggccgct ccaaggtctc cgcccagcgt acgtaacttc
    20401 ttagtcccaa acagcagaac gaccagcgcc gcaactacca gcagtttggt aatactaatc
    20461 tcacccatag ataccttctt gacatataac cggcaacaga acgcgcctta aaaaccagag
    20521 aatattaacg gtcattcgac gcgcgcacac aatagcaatc tgtaacaagg tgaatcaagc
    20581 gccgagtaaa aaaacatcat aataattgcg gcggcgcaaa gcgacggttg tttaagacgg
    20641 gcagttgcgc gcgcacctga cgcacacgtt cgggcgtcac ttccgccata atgagtgcag
    20701 gcatttctga cgctgccgca atggtgacgc caaagggatc tataatccgg ctttgaccga
    20761 tatttttgtt cccgcactcc cccgccgcca ccatataaca ggtggtatcc agcgcacgag
    20821 cggcaagcaa cgttgaccaa tgatgctctt tgagcggccc gcgaacccag gcggcaggaa
    20881 gtaccaggat ttcagctccc tgtaatgcct gtgccagcgc cagctctgga aagcgtaagt
    20941 cataacaggt catcagaccg accttcatcc cctccacctc gagtaacgga gcgatttcat
    21001 taccagcatc aacacggcgt gattcctgaa tggcaaatgc atcatagaga tgcaatttgg
    21061 cataacgggc gacgatgttt cctgcctgta gcgccaccag catattccat gcgcgccccg
    21121 gcgttgaagg aacatgaatc gtcagaattg tcgtcatcat gttacgttta ctttctcgcc
    21181 gtaaacgtcc gaggaattcg ccttccagca gctgtgctga tttaaccgat agatctgcat
    21241 catgatcatc gcgcgccagc aatgcttccg gcaggacgaa cagcgatacg tcgttttccg
    21301 ccgcctgcgc catcaacgag gcacaaatct cagcgttctt ttcccacaca gatgtaacag
    21361 caaactgtcc tgcggcaacc agcattgcca cctcctgtca gagtcgtaaa attcgccaac
    21421 gacgattatt cggcgttaca cttatcactc atacaaatca aatagcagga ttttgcagtg
    21481 ttacaacttc ttttagcagt ttttattggc ggtggtacgg gaagcgtggc gagatggctg
    21541 ttaagtatgc gatttaaccc gctgcatcag gcgattccgt tggggacgct ggcagcaaac
    21601 ctgattgggg cattcatcat aggaatggga ttcgcctggt tcagcaggat gacgaacatt
    21661 gatccagtgt ggaaagtatt aatcaccacc ggattttgtg gcggtctaac aaccttctca
    21721 acattttcgg cagaagtggt gtttttgtta caagagggcc gctttggctg ggcgttactg
    21781 aacgttttcg tcaaccttct ggggtctttt gccatgacag cactggcttt ctggctgttt
    21841 tcggcctcaa ccgcacacta aagaccaaaa aaaaacccgc tgattaagcg ggttttgaat
    21901 tcttgctgac gtatcttaca gagcgattac gtttgcagca gaagggcctt tggcaccgtt
    21961 agtgatttcg aactctacgc gctgaccttc agcaagagtt ttaaaaccat tagtctggat
    22021 tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc ggagtaatga aaccgaatcc
    22081 tttggactca ttaaaccact taacgttacc tttaatctta gacatcaaaa ttacctttac
    22141 atgaaaaata gacacaaatg ctgtgtcggt taccagtaca ccaattgtgg tacgctttgt
    22201 ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat ttttgatgtt acttatttta
    22261 gctattgatt tttaagaagt tactaaaact tcatttgtct caaaactgaa agcgcatcca
    22321 ggcaaagtac acattgccat tgttgtaggt acccggaatg taggtcatct gaaaagtcac
    22381 tgggccataa cccacggagg ccaatggcag tagaaccggg agagggatgt aattccagtt
    22441 atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa tgaaaatttt catccgccag
    22501 cggtcgccag gtactttccc atccgtatcc ggcaatcggt tcccatttgt tccacgagtc
    22561 cttaaatgcc atggcataca ggccatgcca gtttcctttt tcatcccaac gcgacaggcc
    22621 aaaaccgcca ccccacggtc gctcgttata gcgatcggtt ttttctttgt cgtaagcgaa
    22681 acgtgcatgc caggtgatgg caggaatata taaatcataa tgttcaggct gttgccaggt
    22741 ttgtgcaata ttttctctaa atgttgtcat ccactcatct gcgttagcaa aaactttacc
    22801 aacgctgatt aactgaataa aaacaaagga aaagatagcg acatatttac tcacgttcat
    22861 ttgtgaccat aaaacattta tcaaaaatct actactagca tagcaaagct acaattaaca
    22921 taaccttaat agacccaaca taaagaataa tctgaatagc tgctctattt atttccgctt
    22981 gttcaagtta aatatttccg cgcatcgatt aaagatgatg cgtcgttttc tgcgatggga
    23041 atagtcaaaa aagaaaaacc aagtcttttt tgatgacaaa atgcaatcaa ggaaaaatta
    23101 ttttattttt taacgagata caacaatcat cttaacgaag tatataatat atatcgtcaa
    23161 cctatgctca tattagatta ttttttgagc gcacagccgc agctaacacc attgcaatta
    23221 acaaatttgc atcaatccac catcaatttg cacacattat tatgtgataa ttgccaaccg
    23281 ctaaatatgc gttttgttat ctatgtataa aaacagcaac ttcaatgtct taatggcagt
    23341 ttttcttgat tttaatcagc attcatcgcc aatttattgg gcatattttt tccttaagct
    23401 ttaggaattt tttattattt actttggggc ctggagacag gaaaaattat gctgacattc
    23461 attgaactcc ttattggggt tgtggttatt gtgggtgtag ctcgctacat cattaaaggg
    23521 tattccgcca ctggtgtgtt atttgtcggt ggcctgttat tgctgattat cagtgccatt
    23581 atggggcaca aagtgttacc gtccagccag gcttcaacag gctacagcgc cacggatatc
    23641 gttgaatacg ttaaaatatt actaatgagc cgcggcggcg acctcggcat gatgattatg
    23701 atgctgtgtg gatttgccgc ttacatgacc catatcggcg cgaatgatat ggtggtcaag
    23761 ctggcgtcaa aaccattgca gtatattaac tccccttacc tgctgatgat tgccgcctat
    23821 tttgtcgcct gtctgatgtc tctggccgtc tcttccgcaa ccggtctggg tgttttgctg
    23881 atggcaaccc tatttccggt gatggtaaac gttggtatca gtcgtggcgc agctgctgcc
    23941 atttgtgcct ccccggcggc gattattctc gcaccgactt caggggatgt ggtgctggcg
    24001 gcgcaagctt ccgaaatgtc gctgattgac ttcgccttca aaacgacgct gcctatctca
    24061 attgctgcaa ttatcggcat ggcgatcgcc cacttcttct ggcaacgtta tctggataaa
    24121 aaagagcaca tctctcatga aatgttagat gtcagtgaaa tcaccaccac tgctcctgcg
    24181 ttttatgcca ttttgccgtt cacgccgatc atcggtgtac tgatttttga cggtaaatgg
    24241 ggtccgcaat tacacatcat cactattctg gtgatttgta tgctgattgc ctccattctg
    24301 gagttcctcc gcagctttaa tacccagaaa gttttctctg gtctggaagt ggcttatcgc
    24361 gggatggcag atgcgtttgc taacgtggtg atgctgctgg ttgccgctgg ggtattcgct
    24421 caggggctta gcaccatcgg ctttattcaa agtctgattt ctatcgctac ctcgtttggt
    24481 tcggcgagta tcatcctgat gctggtattg gtgattctga ccatgctggc ggcagtcacg
    24541 accggttcag gcaatgcgcc gttttatgcg tttgttgaga tgatcccgaa actggcgcac
    24601 tcttccggca ttaacccggc gtatttgact atcccgatgc tgcaggcgtc aaacctgggt
    24661 cgtaccctat cacccgtttc tggcgtagtc gttgcggttg ccgggatggc gaagatctcg
    24721 ccgtttgaag tcgtaaaacg cacctcggta ccggtgcttg ttggtttggt gattgttatc
    24781 gttgctacag agctgatggt gccaggaacg gcagcagcgg tcacaggcaa gtaaatagta
    24841 atgccggagg caagtttcct ccggctttat catgtcaccc actgtggtat atgcgttgcg
    24901 gtctgccaac tttgccgtga acaatttcag caataatcag atggcggctg gcgcaatatt
    24961 caagataacg cctggcagtg gtgcgactga tggtcagtgc ctgcgccacc gtttctgccg
    25021 tatgttgcac accaggctct ttaaacagtt ttcgcaccgc gtttagcgtc aagggatcaa
    25081 tgccggtcgg cagttcgtct ttaggttcac cgcgagcata ggcattaaac atctcatcaa
    25141 tttgcttctg gctggcgcta tcaatacttt ccagcatatg tttacgctgg cggaaacggg
    25201 ttagcgtttg ccccagccgt tcataggcaa tgggcttaat gagataatca aatacaccac
    25261 aacgtacggc ttcagacacc gtttccatat cgctggctgc agtggtaaac accacgtcgc
    25321 cgggataatg cgcctgcacc agttcatgca ataaattaat ccctctgccg tcaggaagat
    25381 agttatcgag caagattagc cccggcttaa aacgctcgat catcattcgg gcctgcgcca
    25441 ggtttcccgc cagtaatatc tgactgaatc cgggaatgtg acgaatatat tccgcatgca
    25501 tctctgccag cggcgtttcg tcctcaacga tcaatagggt taatggagct gtcattaggt
    25561 ttcactttcg gaatatagat tgaaaataag gtaccgcagg gatcattatc ttcgagagtg
    25621 ataacaccac cgcagcgcgt tacgtagctg gcaatcaagt acaacccaat gccatgttca
    25681 ccgggctcgt cagcacgcgt actgaccccc tgctcaaata ttttgtctcg tagagactct
    25741 ggaacgccgc agccctgatc ggcgacttca atcaccacat catcgccttc atcgctgagg
    25801 aataattcaa cgatcttgtt tccttcatcg ctacgcaggc tggcttcgaa ggcgttatca
    25861 agtaaattgc ccacaatggc tgcaaactcg gtgctatcca gtcctggcgg cagttgcgaa
    25921 agctggctac cggggacaat gatcattttt agccccagtt cccgggcgcg ctgcacttta
    25981 ccaaaaagca gccccgccac ctggcgatcg gcaaacgcct cgcgcaggct gtcaataagc
    26041 tgttgctggg cctgagactc cccctgcacc atcgccagca cgcgatcata ctctttcatc
    26101 tgcaacagac cattgagcgt cgacatccaa ttgagatgct cgtgtcgcaa tgtacgaagg
    26161 ctctcgacgt attgttttat ttgcgtcagt tgcgcattga gggtggaaat ttcgtcttta
    26221 ctacgaaagc tgataatggc ccccagcaaa tcatcacctg aacgaatagc ttcccgattg
    26281 gcaataacgc tcagaccgtt aaagttagcc accacatcct gacgtttttc atcaatctgt
    26341 tcggtaaaga aatcggcggg cctgaccact tcagcaatgg gtttacccaa ccattgccgt
    26401 ccgggagagc tcagccccag catctttctt gcgttacgat tgatggcggt aatgtaacca
    26461 tgcggatcca ccgcaatcag cccttcataa accgaactaa acagcgcctc ttgctgacgg
    26521 accacgcgtg cgatttgctt tggctccatg cccatcattt gccgacggat atgcgctgcc
    26581 aggaaccacg acagcaacat cagaatccct aacagcacga caaacacgcc tgccatcggt
    26641 aataaaaact cagcccgcca gctatcgatt ttactcacca ggtagccaat cgacaccacg
    26701 ccgatgactt ttccatcgtc atcaaagatt ggcgttttgg cgcgcatcgc catccccatt
    26761 gaccctttcc cggtaatgaa gtagctctcc cctttctcca gcgcgccctg tttggtgaac
    26821 tgcataggat aaccaatttt ctccggatta ggatggtaaa ggcggatcga gtgccggtcc
    26881 ccaatcacca cataatcaaa atcggtatct ctttgtaatt tgttagcgat ggtcgccagc
    26941 cgtttgtagt cacgcgtttt caccgcagag atgacactgt cattggaggc aataattttc
    27001 gcctgattca ttgccatgtc gcgtacatga agcgttaaat agtcctcaaa actggccgta
    27061 aaatattgcg ccagggctgc aatgacaaat attgagaaca ccagaatcag caaaaagata
    27121 cgcagcggaa atgccagtct ttggaaaaat gcaaactgtt tattctcgtt aagctgcaac
    27181 attgttttcc ttgcgggttg tcccgagcct ctttgacgga tgtaattaaa atgttgaatc
    27241 aaaatacata gatattaatt atgatcgtgg caaattttat tcattaaatc aattaaatca
    27301 cttaaacctg ctggtcattt cgtaattaca ttaattaaaa aacttaaaac cattaaataa
    27361 ataaaaaacc actaactcta tgtgaaataa atcaaaattt cacgccgaaa tactccttag
    27421 gatgtatagc gaaaggagaa aaagatatac ctcgatcacc cccctttctc ccaagtgaaa
    27481 ataaaaggtt atcagtttgc aacattgaac aacattcgtt gcaaatcgat aacaacatgc
    27541 accttcagga tactatttat tatgttcggc aatgatattt tcacccgcgt aaaacgttca
    27601 gaaaataaaa aaatggcgga aatcgcccaa ttcctgcatg aaaatgattt gagcgttgac
    27661 accacagtcg aagtatttat taccgtaacc cgcgatgaaa agcttatcgc gtgcggtgga
    27721 attgccggaa atattattaa atgcgttgct atcagtgaat ccgttcgcgg tgaaggactg
    27781 gcgctgacat tagccactga actgataaac ctcgcctatg agcggcacag cacgcatctg
    27841 tttatttata ccaaaaccga atacgaggcg ctgttccgcc agtgcggttt ttccacgctg
    27901 accagcgtac ccggcgtgat ggtgctgatg gaaaacagcg ccacgcggct gaaacgctat
    27961 gccgaatcgc tgaaaaaatt tcgtcatcca gggaacaaga ttggctgcat tgtgatgaac
    28021 gccaatccct tcacgaatgg tcaccgttat ctgattcaac aagctgcggc acagtgcgac
    28081 tggttgcatc tgtttttagt caaagaagat tcttcacgct tcccctatga agaccggttg
    28141 gatctggtgt taaaaggcac cgccgatatt ccacgcctga ctgtgcatcg cggctccgaa
    28201 tacatcatct cccgcgctac gttcccttgc tacttcatta aagaacagag cgtcattaac
    28261 cattgttaca ccgaaattga tctgaagatt ttccgtcagt acctcgctcc cgcgctgggt
    28321 gtaactcacc gctttgtcgg tactgaaccc ttttgtcgcg ttaccgccca gtacaaccag
    28381 gatatgcgct actggctgga aacgccgact atctccgcac cgcccatcga actggttgaa
    28441 attgagcggc tgcgttacca ggagatgccg atatccgctt cccgggtacg tcaactgctg
    28501 gcgaaaaacg atctcacggc tatcgcgccg ctggtccctg cagtcacgct gcattatttg
    28561 cagaacctgc ttgagcactc tcgccaggac gcggcagctc gtcaaaagac ccccgcatga
    28621 gaaacaggtg aaaaatgaaa ataaaccagc ccgccgttgc aggcaccctt gagtctgggg
    28681 atgtgatgat acgcatcgcc ccactcgata cgcaggatat cgacctgcaa atcaatagca
    28741 gcgttgagaa acagtttggc gatgcaattc gcaccaccat tctggacgtt ctcgcccgct
    28801 acaacgtgcg cggcgtacag ctgaatgtcg atgacaaagg cgcactggac tgcattttac
    28861 gtgcacgact ggaagccctg ctggcacgcg ccagcggtat cccggctctg ccatgggagg
    28921 attgccaatg atttccgctt cgctgcaaca acgtaaaact cgcacccgcc gcagcatgtt
    28981 gtttgtgcct ggtgccaatg ccgcgatggt cagcaactcc ttcatctacc cggctgatgc
    29041 cctgatgttt gacctcgaag actccgtggc attacgtgaa aaagacaccg cccgccgcat
    29101 ggtttatcac gcgctgcaac atccgctgta tcgcgatatt gaaaccattg tgcgtgtcaa
    29161 cgcgctggat tccgaatggg gtgttaacga cctggaagcc gtcgttcgcg gtggtgcgga
    29221 cgttgtgcgt ctgccgaaaa ccgataccgc tcaggatgtt ctggatattg aaaaagagat
    29281 cctgcgtatc gaaaaagcct gtggtcgtga acccggcagc accggcctgc tggcggcgat
    29341 tgaatctccg ctgggcatta cccgcgcagt ggaaatcgct cacgcttccg agcgtttgat
    29401 cggtatcgcc ctcggtgcag aagactatgt gcgcaacctg cgtacagaac gctccccgga
    29461 aggaactgaa ctgctgttcg cacgctgttc cattttgcag gccgcgcgct ctgcgggtat
    29521 tcaggcgttc gataccgtct attccgacgc taacaacgaa gccggattcc tgcaagaagc
    29581 cgcccacatc aaacagctgg gctttgacgg caaatcgctg atcaacccgc gtcagattga
    29641 tctgttgcac aacctctacg caccaaccca gaaagaagtg gatcacgccc gccgcgtcgt
    29701 agaagccgct gaagccgccg ctcgcgaagg cctcggcgtg gtttccctga acggcaagat
    29761 ggtggacggt ccggttatcg atcgcgcccg tctggtgctc tctcgtgcag aactttccgg
    29821 catccgcgaa gaataaggca atcaaaatga cgcagaaaat tgaacaatct caacgacaag
    29881 aacgggtagc ggcctggaat cgtcgcgctg aatgcgatct tgccgctttc cagaactcgc
    29941 caaagcaaac ctaccaggct gaaaaagcgc gcgatcgcaa actgtgcgcc aacctggaag
    30001 aagcgattcg tcgctctggt ttacaggacg gcatgacggt ttccttccat cacgctttcc
    30061 gtggcggtga cctgaccgtc aatatggtga tggacgtcat cgcgaagatg ggctttaaaa
    30121 acctgaccct ggcgtccagc tccctgagtg attgccatgc gccgttggta gaacacattc
    30181 gccagggcgt ggttacccgc atttatacct ccggcctgcg cggtccactg gcggaagaaa
    30241 tctcccgcgg tctgctggcg gaaccggtgc agatccactc tcacggcggt cgtgtgcatc
    30301 tggtacagag cggcgaactg aatatcgacg tggctttcct cggcgtcccg tcctgtgatg
    30361 aattcggtaa tgccaacggc tacaccggta aagcctgctg cggctccctc ggctatgcaa
    30421 tggttgatgc cgacaacgca aaacaggttg tgatgcttac cgaagaactg ctgccttatc
    30481 cgcataatcc ggcaagcatt gagcaagatc aggttgattt aatcgtcaaa gttgatcgcg
    30541 ttggcgatgc tgcaaaaatc ggcgctggtg ctacccgtat gaccactaac ccgcgcgaac
    30601 tgcttattgc ccgtagcgct gcggatgtga ttgtcaactc aggctacttc aaagaaggtt
    30661 tctccatgca aaccggcacc ggcggcgcat cgctggcggt aacccgtttc ctggaagaca
    30721 aaatgcgtag ccgcgatatt cgcgccgact tcgccctcgg tggtattacc gcgacgatgg
    30781 ttgatctgca cgaaaaaggt ctgatccgca aactgctgga tgtacagagc tttgacagcc
    30841 atgctgcgca atcgctggcc cgtaacccca atcacatcga aatcagcgcc aaccagtacg
    30901 ctaactgggg ttcgaaaggc gcatcggttg atcgtctcga cgtggtggta ctgagcgcgc
    30961 tggaaattga cacccagttc aacgttaacg tgctgaccgg ctctgacggc gtactgcgtg
    31021 gtgcttccgg tggtcactgc gataccgcga ttgcctctgc gctttccatc atcgtcgcgc
    31081 cgctggtacg cggtcgtatt ccgactctgg tggataacgt gctgacctgc atcaccccgg
    31141 gctccagtgt cgatattctg gtcacagacc acggtatcgc agttaacccg gcacgtccgg
    31201 aactggcaga acgtctgcag gaagcgggca ttaaagtggt ttccattgag tggctgcgcg
    31261 aacgtgcgcg tctgctgacc ggtgaaccac agccgattga attcaccgac cgcgtcgttg
    31321 ccgttgtgcg ttaccgcgat ggctcggtga tcgatgttgt gcatcaggtg aaggaataag
    31381 ccatgcacct gcttcctgaa ctcgccagcc accatgcggt gtcaattccc gagctgctcg
    31441 tcagccggga tgaaaggcaa gcacggcaac acgtctggct caagcgccat cctgttccac
    31501 tggtctcctt taccgtggtt gcgcctgggc cgattaaaga cagcgaggtc acacgccgaa
    31561 tttttaatca tggcgtgaca gccttgcgtg ccttagccgc aaaacagggc tggcaaattc
    31621 aggagcaggc tgcactggtt tccgccagcg ggccggaggg catgttgagc attgccgccc
    31681 cggctcgcga cctcaagctc gccaccattg agcttgaaca tagtcatcct ctcgggcggt
    31741 tatgggatat cgatgtcctg acgcccgaag gcgaaattct ctcccgccgc gactattcac
    31801 tgccgcctcg ccgctgcctg ttgtgcgagc aaagcgcagc cgtctgcgcg cgtggaaaaa
    31861 cccatcaact gaccgattta ctcaaccgca tggaggcact gctgaacgat gtcgatgcct
    31921 gcaacgtcaa ctaaaaccac aaagcttgcg acgtcattaa tcgatgagta cgccctgctg
    31981 ggctggcgcg ccatgctgac tgaagtcaat ctgtcaccga aaccaggcct cgtggatcgc
    32041 attaactgcg gtgcgcacaa agatatggcg ctggaagatt tccaccgcag cgcgctggcg
    32101 attcagggct ggttaccccg tttcattgaa tttggtgcct gtagtgcaga aatggcaccg
    32161 gaagcggtac tccacggatt acgcccaatt ggtatggctt gcgaaggtga tatgttccgc
    32221 gccactgcgg gcgtaaacac gcataaaggc agcatttttt ctttagggct gctatgtgcg
    32281 gcaattggtc gtttgcttca actcaaccaa ccggtaacgc caacaaccgt ttgttctacg
    32341 gcggcaagtt tctgccgtgg cctgaccgat cgagaactgc gtaccaataa ttcacaactg
    32401 acggcaggtc aacggttgta ccaacagctt ggccttaccg gcgcacgcgg tgaagccgaa
    32461 gcgggttatc cactggtgat caatcacgcc ttgccgcatt acctcactct gctggatcag
    32521 gggttagatc ctgaactggc attgctcgat accttgctcc tactgatggc gatcaacggc
    32581 gataccaacg ttgcatcgcg cggtggcgag gggggcctgc gctggctaca gcgcgaggcg
    32641 caaacattat tgcaaaaagg gggcattcga acccccgccg atctcgatta tctccggcag
    32701 ttcgacaggg agtgtatcga acgaaatctc agtccaggcg gcagtgccga cctgctgatc
    32761 cttacctggt ttttagcaca gatttaatta tttaagcact tgataaattt ggaaatatta
    32821 attttcggag aacccgtatg tctttagcaa aagataatat atggaaacta ttggccccac
    32881 tggtggtgat gggtgtcatg tttcttatcc ctgtccccga cggtatgcca ccacaggcat
    32941 ggcattactt cgctgtgttt gtggcaatga ttgtcggcat gatcctcgag ccaattccgg
    33001 caacagcgat cagttttatt gcggttacta tttgcgttat tggcagtaat tacctgctct
    33061 ttgatgccaa agaattagct gacccagcgt ttaatgcgca aaaacaggcg ctgaaatggg
    33121 gtctggctgg tttttccagc accacggtat ggctggtatt tggcgcattt atttttgcat
    33181 tagggtatga agtttccggg ttaggtcgtc gcattgccct tttcctggtg aaattcatgg
    33241 gcaaacgcac gctgacgttg ggttatgcga ttgtcattat cgacattctg ctggcaccgt
    33301 ttacaccgtc caacaccgcg cgtaccgggg gtacggtttt tccggtcatt aaaaacctgc
    33361 cgccgctgtt taaatcattc cccaacgatc cgtccgcgcg tcgtattggc ggctatttga
    33421 tgtggatgat ggtcattagt accagtctga gttcgtccat gtttgtcacc ggtgcggcac
    33481 caaacgtgct gggtctggag ttcgtcagca aaattgccgg tatccagatt agctggttgc
    33541 agtggttcct ctgcttcctg ccggttgggg ttatcttgct tatcattgcg ccgtggcttt
    33601 cctacgtgct gtacaaaccg gaaatcacac acagtgaaga agtggcaacc tgggcgggtg
    33661 atgaactaaa aaccatgggt gcgctgacac gcagagagtg gacgctgatt ggccttgtat
    33721 tgctcagctt aggtttgtgg gtatttggca gtgaagtcat taatgctact gcggttggtc
    33781 tgctggcagt ttcgctaatg ctggctctgc acgttgtgcc gtggaaagac attacccgct
    33841 ataacagcgc atggaacacg ctggtcaacc tggcaactct ggttgtgatg gctaacggcc
    33901 tgactcgttc tggttttatt gactggttcg ccggtaccat gagtacgcac ctggaaggat
    33961 tctcaccaaa cgcaacggtg attgtactgg ttctggtgtt ctactttgca cactacctgt
    34021 ttgccagcct gtctgcgcac accgcaacca tgctgccggt tattctggcc gtcggtaaag
    34081 gtattccggg cgtaccaatg gaacaactgt gtatcctgct ggtgctgtct atcggtatca
    34141 tgggctgtct gacgccgtat gcaaccggtc ctggggtgat tatttacggc tgtggctatg
    34201 tgaaatcaaa agattactgg cgtcttggcg caatcttcgg ggtgatttac atctctatgt
    34261 tgctgttggt tggctggccg attctcgcca tgtggaacta atacgttcgc caaacgctga
    34321 ataaatcatc acgcccgcca ggtaagccca cctggcgggc ttttttatga tttaatagat
    34381 agtattcagt gtatgtatga gttccacacc cattatgaaa gcattctggc gtaacgccgc
    34441 gttgctcgcg gtttctctgc ttcccttctc ttctgccaac gccttagcgt tgcaggcaaa
    34501 acagtatggc gattttgatc gctatgtcct ggccctctcc tggcaaaccg gattttgcca
    34561 gagtcaacac gatcgaaatc gtaacgaacg agatgaatgt cgcctgcaaa ccgaaacgac
    34621 caacaaagct gattttctga ccgtacatgg tctgtggcca ggattgccta aatcggttgc
    34681 tgcccgtggt gttgatgaac gccgctggat gcgcttcggt tgcgctactc gcccaatccc
    34741 gaatctacca gaagcgcgcg ccagccgaat gtgttcatcg ccggaaaccg gattatcact
    34801 ggaaacggcc gctaaactaa gtgaagtcat gccaggagct ggcggacgtt cctgcctgga
    34861 acgctacgaa tatgccaaac acggtgcctg ctttggtttt gatccggacg catacttcgg
    34921 tacgatggta cgcctgaatc aagaaattaa agagagcgaa gccggaaaat tccttgcgga
    34981 taattacggt aaaacagtga gccgccgtga ctttgacgcc gcctttgcca aaagctgggg
    35041 aaaagagaac gtgaaagcag ttaagctaac gtgccagggt aaccctgcgt atttgactga
    35101 aattcagatc tcgatcaaag ctgacgccat caacgctccg ctttctgcaa actcattctt
    35161 gccacaacca cacccaggca actgtggcaa aacctttgtg attgataaag tgggttatta
    35221 atcatctgat ttacaaaaca cgctaattcg tcttccataa accgtgacga ttaagaatta
    35281 tacataatgc gctgcattga ggaattccct gaatgtgacg caaatcactt caaagagtga
    35341 gcaacagtca gtatcatcat gtgagttaaa ccctcgccgc ctgacggtga gggttttctt
    35401 ttgggtatgt ttatctgcga cactcgcagt accgacgaca tggagtaaaa atgtccagac
    35461 caactatcat cattaacgac ctggatgccg aacgcatcga tattctgctg gagcaacccg
    35521 cctatgctgg tttgccaatc gccgacgcgt taaacgcaga gttggatcgc gcccaaatgt
    35581 gttcgccaga agagatgcca cacgacgtgg tgacaatgaa cagccgggtt aaattccgca
    35641 atcttagcga tggcgaagtg cgtgtgcgca cgctggtgta tccggcaaaa atgaccgata
    35701 gcaatactca gctttccgtt atggctccgg taggtgccgc actgctgggg ctgcgcgttg
    35761 gcgattccat tcactgggaa cttccgggcg gcgttgcaac ccaccttgaa gtgctggaac
    35821 tcgaatacca gccagaagct gctggcgact acctgcttta atcccatctg cacaagcgat
    35881 aactcgcttt gcccagagga tgcactcgca tcctctttac gcaaaatcag caatttttcc
    35941 tggttctcac tgttcacttt tgctgcaaaa ttattccgtt tcgtttgtgg gaataaccat
    36001 ggcagaagca ttttacatcc tgataggatt tctgattatg gcggcgatta tcgtcatggc
    36061 tgtcctttac ttcgaaaacc actcctgacg atcatattgt tccccccggc atcgcattca
    36121 ccagacctga aaccgccttc tgcgccgcct ctgcgctttg tgcaccgggt accagaatca
    36181 ccttacggca ctcctcttca cgtttttcga aaatctcata tccccgggcg gcctcttcaa
    36241 acggcatata gtgggtaaca atttcttctg gtttcagtaa tcctttctca attaacggta
    36301 ataattctcc cagccatgcg tgaacgtggg tctgtcccat tttaaacgtc aaccctttat
    36361 caaaggcgtc gccaaacagg aaaccgtgaa taaatccagc gtagacgccc ggtacgctaa
    36421 caatgccgcc acgcctgacc gccgcaatac actggcgcaa cgctttaccg ctgctgccct
    36481 ccagtttcag gttagtcagc accgtttccg tggtgctgcc tttcgcttca aaaccgacgg
    36541 cgtctattac tgcatccacg ccccggtgac ctgccgtttg ttcaataatt gactgtgccg
    36601 gatcgctatc ttcatcgaaa ttaatcggga tcgcgccgta gcggtcagcg gcgaaacgca
    36661 agcggtaggg atgatgatca acaacaaaaa tctgttccgc accgagcaac cgtgcacagg
    36721 cgattgtcaa caatcccact ggaccggcac catagactgc aacgcttgaa ccttgttgga
    36781 tctgcgcatt ttttgctgcc tgccatgccg ttggcagaat atcagaaagg aaaagggctt
    36841 tatcatctga aagcaaaggc ggtactttaa acggccccac attcccttta gggacgcgga
    36901 catattccgc ctgcccacca ggaacgccgc catacaggtg actataacca aacaatgccg
    36961 cgggagctgg tatctgtttt ttattgagcg cagcgccttt acccgcattg gtattttcgc
    37021 aggcggcata ttgctgtaat cgacagaaaa aacagtcgcc acaagcaatg acaaacggaa
    37081 ttaccacccg gtcgcctttt tgcaaatttt ttacgtcctt tccggtttca acgacttccc
    37141 ccataaattc atgaccaaaa atatcgccat gtttaacctg gggtattttg cctcgataaa
    37201 gatggaggtc agagccacag atcgccgttg ccgtaatacg cagaataata tcatctgcct
    37261 gttcaatgcc cggatcggga acattttcta cctgaacgtg atgtgggccg tgataagtca
    37321 atgctttcat gcaacctcca gagacagttc aaaaaagagt cagtctttaa gggtagctat
    37381 cacagcggga aaacgggcgg taccgcaggt tttgggagaa ttcctggtgt tcagtgagaa
    37441 tatattaatc acagaaaccg ttcgtaagga aaaagaacca gacaaatatc agggataaat
    37501 attataatca tggcgaatga tttgttcatg attaacaggg agaaagggtt atgtataaga
    37561 caatcattat gccagttgat gtatttgaaa tggaattgag cgacaaagct gttcgccacg
    37621 ctgaattcct cgcccaggat gacggagtta ttcatctact tcacgtacta cccgggtcag
    37681 ccagcctgag cctgcaccgt tttgccgctg atgtgcgtcg ttttgaagag catctgcaac
    37741 atgaagcaga agaacgtctg caaacgatgg tcagccactt caccatcgat ccttcccgca
    37801 ttaaacaaca tgtccgtttt ggtagcgtgc gggatgaagt caatgagttg gcagaagaac
    37861 tgggggctga tgttgtagtt attggttctc gcaacccatc gatttcgacc catctgttag
    37921 gttctaacgc ctcgagcgtg atccgccatg ccaatctgcc ggtgctggtt gtgcgttaat
    37981 tgccagaata aatatcccgc cctgcccgga gccatcccgg cagggctttt taatgccata
    38041 aaaaagccgc caggtttgac cccggcggct aagcaattgc aggtgaatct tacttcttct
    38101 tatgcagttt tggtgcgaat caggtagtca aaagcactca gagaggcttt ggcaccttcg
    38161 ccagtggcga tgatgatctg cttgtacgga accgtcgtac agtcacccgc tgcgaatacg
    38221 cctttgacgt tggtttcgca tttcgcatca atgataatct cacccatgcg gttacgttcg
    38281 actgcgcctt ccagccagtt ggtgttcggc agcagaccaa tctggacgaa aataccggcc
    38341 agttcgatgt tgtgaatatc gccgctgaca cgatctcgat attccagacc aacgactttg
    38401 ctgccgtcgc ctttcacttc cgtggtttgc gcattcagaa taatgtcgac gtttttcagg
    38461 ctgcgcagtt tgtcctgcag cacctggtcg gctttcattt ctggcgcaaa ttccagcaga
    38521 gttacgtgct caacgatacc cgccaggtcg attgccgctt ccacgccgga gttaccgccg
    38581 ccgataaccg ctacgcgttt gcctttaaac agcgggccgt cgcagtgcgg gcagtaggtc
    38641 acgcctttgg tgcgatactg atcttcgccc ggaacgttca tgttgcgcca ttttgcaccg
    38701 gtcgccacga taatgctgcg tgctttcagt accgcgccag aagctgtttc aatctggtgt
    38761 agaccacctt caaccgctgc cgggatcagt ttgctggcgc tctggctgtc gatcacatca
    38821 acgtcgtatt catcaacgtg aactttcagt gcgcctgcca gcttctgccc ttcagtcttc
    38881 ggtacagaaa tgtagttttc gatatcaacg gtatcgagga tctgaccacc aaaacgttcg
    38941 cccatcagac cggtacggat gcctttacgt gcggagtaaa ttgctgccgc tgcacccgcc
    39001 gggccggaac cgacgattaa tacgtcataa gcatcacgct tgttcagctc ttctgccgca
    39061 cgtttttccg cgccagtatc aattttggca acgatttcag tcaacgtcat gcggccctga
    39121 ccaaactctt tcccgtttac gaacactgcc ggaacgccca tcacgttgcg atcggtgatt
    39181 tcgttctgga aggtgccgcc gtcaattgca gtgtgcttga tgcgcgggtt cagtacgctc
    39241 atcaggttca gcgcctgcac cacgtccggg cagttgtggc aagagagcga gtaataggtt
    39301 tcgaattcaa aatcaccgtc aatatggcga atctgctcca gcagagactg cgcttctttc
    39361 gacggatgac caccggtcca cagcaacgcc agtaccagcg aggtgaactc gtggcccagc
    39421 ggggagcctg caaaacgtgg cccctggttg gaacctgggt tggtgatcag gaaagacggc
    39481 ttacgcaccg gcaagctgtt atcttcttta aaggtgactt tgtctgacag ttctgcgatt
    39541 tcagccaaca gttccttgat ttctgccgat ttagcgctgt catccagcgt ggcaattaac
    39601 tcaacaggct tggtcaattt ctcaaggtaa gccttgagtt gagttttcat atttgtgtcg
    39661 agcatgttta tctcctgggc tttaaacatc atcatgcaag cggcctctgc ggctacctgg
    39721 atgcagcttg catcatggtg cggggaagaa aatcgggtgc cgcagcaccc gattaattac
    39781 gcgattgcgg aacttagatt ttaccaacca ggtccagaga cggagccaga gttgcttcac
    39841 cttctttcca tttagccggg caaacttcac ctgggtgaga agctacgtac tgtgctgctt
    39901 tgattttacg cagcaggtca gacgcgtcac ggccaatgcc ttcagcggta acttcgattg
    39961 cctggatgat accctgcggg tcaacaacga aggtcgcacg gtcagccaga ccttcatctt
    40021 cacgcatgtt gtcgaagtta cgggtcaggg cgccagtcgg gtcgccgatc atcgcatatt
    40081 tgattttagc gatggtttca gagctgctgt gccatgcttt gtgggtgaag tgagtatcgg
    40141 tagatactgc gtatacgtct acgcccagtt tctgcagttc ttcgtagtgg tcagcaacgt
    40201 cacccagttc ggtcgggcat acgaaagtaa agtcagccgg gtagaagaag aagacgctcc
    40261 agcggccttc ggtatctttt tcggtgattt cgatgaattc gccgtttttg aatgcctggt
    40321 ttttaaaagg tttaattttg gtgttaatca aggacatcta tacttcctcc gtgttttcga
    40381 tgagatgtaa ggtaaccaat ttttgccgat tcggctaatg cgtttccatt atcaaatcga
    40441 taagttttac cttacaacct tcgtaagaca actttgtgaa ctttgcaccg ccagcggcga
    40501 taaaaagtaa aatggccccc tttccaggtg accgtgtaat ctgaactacc ctttggtatc
    40561 ttcagcgcct gcctgcaggc tgaggtgttg cccttacaaa tgcaacaacg acatggatta
    40621 caacaccctc ataaacaaag ggcaatcacc tgatctaagc tcttacctat gacagtgata
    40681 ggttatgcct tttactcgac ttttgcactg actgaaaagg acaaattaat gttaaaaaag
    40741 atacttttac tggctctgct tcctgcaatc gccttcgcag aggaacttcc ttccccagta
    40801 aaagcgattg aaaaacaggg cattacaatc atcaaaacgt tcgatgcccc cggaggaatg
    40861 aaaggttatc tcggaaagta tcaggatatg ggcgtcacca tctacctgac tcccgatggt
    40921 aagcacgcta tctctggtta catgtacaac gagaaaggtg aaaacctgag taacacactt
    40981 atcgaaaaag aaatttacgc accagccgga cgcgaaatgt ggcaacggat ggaacaatcc
    41041 cactggctcc tcgacggtaa aaaagatgcg ccggtcattg tctacgtgtt cgccgatccg
    41101 ttctgcccat attgtaaaca gttctggcag caggcgcgcc cgtgggtaga ttctggcaaa
    41161 gtgcaattaa gaacattgtt ggttggggtt atcaagccag aaagcccggc gacagcagcg
    41221 gcaattcttg cctccaaaga tcccgcaaaa acctggcaag aatatgaatc ctctggtggc
    41281 aagcttaagc taaacgtgcc tgcaaacgta agtacagaac aaatgaaagt gttaagcgcc
    41341 aacgagaaac tcatggacga tctgggggca aatgtcacac cagcgatcta ttacatgagt
    41401 aaggaaaata cgctgcaaca ggccgtgggg ttgcccgatc agaaaacgct taatatcatt
    41461 atggggaata aataaaagat aattcatgga tggttgaaat acatccctgc ttttcaacca
    41521 tcctaaaaaa gcatgaattg ctcgctctgc cctaagtggc tatgctattt tagaaaaact
    41581 taatcctgag ttaagcaaaa aaatataatc aataaaaata tatacgcaaa aaacattgat
    41641 taagtgaata taccatggaa gaaaaatata accggagtag tgtatggcca atctctacga
    41701 cttgaaaaag ttcgacttaa accttcttgt catatttgag tgtatttacc agcatttaag
    41761 cattagcaaa gctgctgaat cactatatat caccccatca gccgtgagcc agtcgttaca
    41821 acgcttacgt gcgcaattta atgacccttt attcattcgc tcaggaaaag gtatcgcgcc
    41881 gacgacaaca gggttgaatc tgcatcacca tcttgagaaa aacctcaaag gccttgaaca
    41941 aacaattaat atagtcaata agtccgaact aaagaaaaac tttattatct acggccctca
    42001 gctcatttct tgctctaata atagtatgct gatccgcagc ctacggcaag atgcctctgt
    42061 cgagattgag tgccatgata tcctcatgtc agcagagaat gctgaagaac tgttggttca
    42121 ccgcaaagcc gatctggtga tcacccaaat gccagtgatc agccgttccg tcatttgcat
    42181 gccattgcat acaattcgca atactctaat ttgtagtaat aagcatccgc gaattactga
    42241 caatagcact tatgaacaaa ttatggcaga agagttcacc caattaatat ccaaatcagc
    42301 aggagttgat gatattcaaa tggaaataga cgagaagttt atgaatcgca aaatttcatt
    42361 tcgtggttcc tctttgctga caatcattaa tagcattgca gttacagatt tactgggaat
    42421 agtcccctat gaactttata attcctaccg tgactttctt aatttaaaag agataaaact
    42481 agaacatccg ttaccttcaa taaagcttta catttcctat aataaatctt ctttaaataa
    42541 tttggtgttc tcccgattta ttgaccgtct aaatgagagt ttttgatata taactacaga
    42601 cagctactat aactttcatc tatttattca cagcgcgaat gaaaaataaa acactctgct
    42661 ttgcagtgtt tactttataa ttaacagact tatctcactg atcaccctgt aacgttcaga
    42721 gagcgtcttc agatgtctat ttataaaatt cctcttccgc tcaatattct ggaagctgca
    42781 cgagagcgta ttacctggac actcaacacc ctaccccgcg tttgtgtctc attttctggc
    42841 ggcaaagact ctggtttaat gctgcatctg acagccgaac ttgcccgacg aatgggcaaa
    42901 aaaatctgcg ttttgtttat cgactgggag gcgcaattct cttgcaccat taactatgtt
    42961 cagtccctgc gcgagttgta caccgatgtc atcgaagagt tttactgggt tgcgctcccg
    43021 cttacgacgc aaaattccct ttcacaatac caacccgaat ggcagtgctg ggaacctgat
    43081 gtcgaatggg tgcgtcagcc cccgcaagat gcgataaccg atcctgactt tttctgcttt
    43141 taccagcctg gcatgacctt cgaacaattt gtacgtgagt ttgccgaatg gttttcacaa
    43201 aaacgtccgg cggcgatgat gatcggcatc cgtgcggatg agtcctacaa ccgttttgtc
    43261 gccatcgcca gtttaaataa acaacgtttt gccgacgata aaccatggac tacggccgca
    43321 ccaggcggtc atagctggta catttacccc atttacgact ggaaagtggc tgatatctgg
    43381 acctggtatg caaatcatca aagtctctgc aacccactgt ataacctaat gtatcaggca
    43441 ggcgttcctc tgcgtcatat gcgaatttgc gaaccttttg gcccggagca acgacaagga
    43501 ttatggctct atcacgttat cgaaccggat cgctgggcag ctatgtgcgc acgagtcagc
    43561 ggggtaaaaa gtggcggcat ttacgccgga catgacaatc atttctatgg gcatcggaaa
    43621 atcctcaagc cagaacattt agactggcaa gaatatgcat tattgctgct caatagcatg
    43681 ccggaaaaaa cagctgagca ttaccgcaat aaaatcgcca tttatttgca ctggtatcag
    43741 aaaaaaggca tcgaggttcc acaaacccag caaggggaca ttggcgcgaa agatatcccc
    43801 tcctggcggc ggatatgcaa agttttactc aataacgatt actggtgtcg agcattatca
    43861 tttagtccta cgaaagcgaa gaactatcag cgttataacg aacggataaa aggaaaacgt
    43921 caggaatggg ggatactatg caacaacgat taacgcagga tttaacgcaa tttctcgcca
    43981 gcttgccgga agatgatcgc atcaaagcga ttaatgaaat ccgcatggcg atccatcagg
    44041 ttagcccctt tcgtgaagag ccagtggatt gcgttctgtg ggttaaaaac agccaactta
    44101 tgcctaatga ttacaaccca aacaacgtgg caccacccga aaaaaaactg ctgaaaaaat
    44161 ccattgaaat agatggtttt acccaaccca ttgtggtcac gcatacggat aaaaatgccc
    44221 tggaaatcgt ggatggtttt caccgccatg aaattggtaa aggctcatca tcattaaaat
    44281 tacgcttaaa aggttatttg ccggtgacct gtctggaagg tacgcgtaat caacgcatcg
    44341 ccgcgactat tcgtcataat cgcgctcgtg gacgccatca aatcaccgcc atgtcggaaa
    44401 tcgtccgtga acttagccag ttgggatggg acgataataa aattggcaaa gagctaggaa
    44461 tggacagcga cgaagtgctg cgcctgaagc aaattaacgg cctgcaagag ctgtttgccg
    44521 accgtcaata ttcccgcgcc tggacggtta aataactaaa gctggcgcag gcgttcagct
    44581 gctgccagca acgtcgattc cttcttggca aaacagagac gaatcagttt atgtgggaag
    44641 ggatcggcgc aaaacaccga cagcggaatc gccgctacgc cgtgctcctg cgtcagccac
    44701 tggcaaaact caacatcatc cagggtagaa acctcgctgt aatccaccag caaaaagtat
    44761 gtaccttcac acggtaaaat ctccagccgg ctttcattta aagcattcac cagaatatcg
    44821 cgcttctggc gataaaagtc cggtaacgca agataatgct caggttctgc acgtagcata
    44881 tcagcaagcg ccagctgtgc cggggtattc accgaaaagg tcagatactg atgtaccttg
    44941 cgaatttcgg cgctgatggg cgctggcgca acacaataac ccactttcca gccggtcata
    45001 tgataggtct tgccaaatga agaaaccgcc actgcccgct cacgcagctg cggatgcgcc
    45061 agcacactgg catggccctg ttgtgaaaag ttgatgtgct cgtagacttc atcgctaatg
    45121 acaaaaatct cgtgcccggc gatcgcctgc cacaaagcgg cgaaatcagc ctgctgccag
    45181 acagttgcac tggggttatg cggagtgttg aggatcacca gtctggtgcg ctcgcttaat
    45241 aatgcggcaa attcctgcca gtcaacgcga aaatgcggtg gttgcagtgc catacgcttc
    45301 actattcccc cagaaagcgc gatggcgggg gagtaactgt catagctggg atcaaaacaa
    45361 atcacttcat cgccattgcg caccagtgcg gtaatcgccg cgtataacgc ttccgtcgcc
    45421 cctgccgtta cggtgatatc gctatcggca tctggttgat agccatacaa acgttccgtt
    45481 ttctgagcaa tcgcctcgcg caaggcctgc acgccggtca tgggcgcgta ttggtttgcc
    45541 ccctgggcaa cgtggtacgc cagccgctcc tgtaaatagc gcggaccatc aaaatcagga
    45601 aagccttgcg acaggttaat cgcctggtgt tgctgcgcca gcgcgctcat ctgggtgaaa
    45661 atagtggtgc caagttgtgg aagtttgctt tgtggaatca gagggttatt tgtcatttta
    45721 ttgtacctga cgaagttgcg tgttgaaggc actataacac gatgctagta tttggcaatc
    45781 aagacgttta gatgtctaaa taaaacaata aggacaacac aacatgcctc acaatcctat
    45841 ccgcgtggtc gtcggcccgg ctaactactt ttcacatcca ggaagtttca atcacctgca
    45901 cgattttttc actgatgaac aactttctcg cgcggtgtgg atctacggca aacgcgccat
    45961 tgctgcggcg caaaccaaac ttccgccagc gtttggactt ccaggggcaa agcatatttt
    46021 gtttcgtggt cattgcagcg aaagcgatgt acagcaactg gcggctgagt ccggtgacga
    46081 tcgcagcgtg gtgattggcg tcggcggcgg cgcactgctc gacaccgcga aagccctcgc
    46141 ccgccgtctc ggtctgccgt ttgttgccgt tccgacgatc gccgccacct gcgccgcctg
    46201 gacaccgctc tccgtctggt ataacgatgc cggacaggcg ctgcattatg agattttcga
    46261 cgacgccaat tttatggtgc tggtggaacc ggagattatc ctcaatgcac cgcaacaata
    46321 tctgctggcg gggatcggtg acacgctggc gaaatggtat gaagcggtgg tgctggctcc
    46381 gcaaccagaa acgttgccgc taaccgtgcg actggggatc aataatgcgc aggccattcg
    46441 cgacgtattg ttaaacagta gcgaacaggc gctgagcgat cagcaaaatc aacagttaac
    46501 gcaatcattt tgcgatgtgg tggatgctat tattgctggt ggtgggatgg tcggtggtct
    46561 gggcgatcgt tttacgcgtg tggcggcggc tcatgccgtg cataacggtc tgactgtcct
    46621 gccgcaaacc gagaaatttc tccacggcac caaagtcgct tatggcattc tggtacaaag
    46681 cgccttgctg ggtcaggatg atgtgctggc gcaattaacc ggggcgtatc agcgttttca
    46741 tctgccgacc acgctggcgg agctggaagt ggatatcaat aatcatgcgg agatcgacaa
    46801 agtgattacc cacaccctgc gtccggtgga atccattcat tacctgccgg tcacgctgac
    46861 accagatacg ttacgtgcag cgttcgaaaa agtggaatcg tttaaagcct gacgtacata
    46921 tttagcagca gcgcgcgccg ccttttccac cgtagcgcgc gtcctgccgc tcacggaaaa
    46981 actcctcgta ggtcatcggc gtttgatcgg gatggttaac ccgcatatgt tcgacatagt
    47041 tgtcgtaatc aggcatgcca atcatcagct tcgccgcctg acctaaatat tttccggctt
    47101 ttgccagtga atcaaacatc ttggcctccg ctaaccatgt tgctgaaaga gtgccggatg
    47161 cggcgtgaac gccttatccg gcctacaaat gctatcaacg cgctcggtca gtcccctcgc
    47221 cccaccgggg agagggtgag ggggaaccct cggcgccatg ccaacattcg ccaacattcc
    47281 ccctcactct gactttagtg tgcgcctttt gcctgcgcca cgatctcctc gacattttcc
    47341 ggcattggct catacggcgt ttctttcgcc gttggcttcg gctctttcaa tgccgcaagt
    47401 gccgtcttaa tcgagaacag tgccagaacc accacgacca ccataaagaa gatggttaac
    47461 ccggcatcca gacggttgtt gaacaccagt tgcgccagct gtgactcagt atactgcgac
    47521 ggaatattgc cgctgtcgat cattgcctgg aacttgttag caatggccag gaagccgact
    47581 ttcgcatccg ggctaaacgc tttctgccag cctgcggtca gggtacaaat cagtagccag
    47641 gccgttggta ccaacgccac ccaggcgtaa cgttgacgtt tcatcttgaa caacaccacg
    47701 gcacagagca tcagcgccat ccctgccagc atctggttgg caataccaaa cagcggccac
    47761 agagtgttaa taccgcccag cggatcgacc acgccctgat ggaggaagta gccccacgcc
    47821 agtacgcaca gcgccgttgc cagcaggtta gcaggcagtg aatcggtccg cttcaggcca
    47881 ggagacacca cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt acccgcatct
    47941 accgccgtca gaataaacag agcttcaaac agaatggcga agtgatacca gaacgccaca
    48001 tccatcatac cacccagcgc accgtgcagg atgtaagcca tccccaccgc cagcgtcggc
    48061 gcaccgcccg cacgggaaat gatcgactgt tcacccactt cgctggcaat ctggttgagc
    48121 gtatctggtg taatgctaaa gccccagcta ctcaccacct gcgcggcaga agcgactaca
    48181 tccgccgtcc ctgccggagc cagcaccgcc attgggctgt tcatggcaaa atacacgccc
    48241 ggatcgatga tacaggcaga aaccagcgcc ataatcgcca cgaaggattc catcaacatc
    48301 ccaccgtagc cgataaagca cgcctgccct tcgttcgcca gcatcttcgg cgtggtgccg
    48361 gaagagatca gcgcatggaa gccagacacc gcaccacagg cgatggtgat aaacaggaac
    48421 gggaacaggt taccggtcca taccgggcca gtgccatcga caaatttggt cagcgcaggc
    48481 atggtcagcg tcgggcgcat aatcaaaatg cctaccgcca gaccgacgat agtcccgatt
    48541 ttcaggaagg tcgagagata gtcacgcggg gccagcagca accacaccgg cagcaccgcc
    48601 gccacaaaac cgtagcccac cagcatccag gtcagctgca cgccggtaaa gtcaaagtac
    48661 ggtgcccagg tcggactttc tgccacccag ccgccagaga taatggcgaa aatcaggaac
    48721 accaggccaa tcactgacac ttcgccaatg cggcccggac gcagatagcg caggtagatt
    48781 cccataaaaa tcgccagcgg aatggtgaac gcaacggtat acgttcccca cgggctatga
    48841 gtcagggctt tcaccacgat catcgccagt actgcaagga taatgaccat gatcataaag
    48901 caggctacca gcgcaatcac cccggcagtt ggccccatct cttctttgac cagctcaccc
    48961 agcgatcgac cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg caccgcacca
    49021 gcgagaacca ccccggcgag cagccagatc atccctggca ggtaacccat ttgcgccgcc
    49081 agtaccggcc caaccaacgg acctgctccg gcaatggccg caaaatggtg accgaacagc
    49141 actttcttgt ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc tggcgtcata
    49201 cgcgtcgggt caaccgccag cacatttttg gcgatataca gcccataaaa acggtaagcg
    49261 atcagataga tacagaccga cgccaccaca atccacagcg cgttgatctg ttccccacga
    49321 tttaaagcaa tgtatcccag agcaaatgct cccattacag agagcactgt ccagacgagg
    49381 tatttccctg atttgttcat agttgttatc cgtgtgcgtg tccatagaga tgttacattt
    49441 tgtttctata acatctttgt acgatttaac aacacggtaa ccatgtaaat cggagttgaa
    49501 accaatattt aacttaacaa tgttaactcg atcactccgt taaccgagtt ttcctgcaat
    49561 ctcatttaat tctgtcgggc tcatcccaaa actgccgtac ccagccgaca agtgcagcaa
    49621 cgccgcccct gttcatcgaa aacgacgatt tcccagctct gattttgccg cccaagatgc
    49681 agcggctggc agacgccgcg taccttgcct tcagacaccg ggcgatgatg ggtggcgtta
    49741 agttccgtac ccaccacaca ctgcccgtca cgggtcatca taaatccggc catcgatccc
    49801 agcgtttcag ccagcgccgc cgacgcgccg ccatgcagca ggccaaacgg ctgatgagta
    49861 cgggtatcaa ccggcatttc ggcttccagc acatcatctc ccagacgggt atacacaatt
    49921 cccagatgcg ccaccattgt gttatcgctg gtggcgttca gttcgtcgag cgttaaatgg
    49981 cgtttccaga tcatgcttat gcccccagcg ttgagccgcc atcgaccaca atatcctgta
    50041 gggtgatatg gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg atctcttgtg
    50101 gacgggcgat tttccccagc ggaatgccga gtttaaactg ctcgccaaag ccgcgaatac
    50161 gctgttcttc ggcgtcatcg ctcacccaca gcgtgcgttg catatcggtg tcggtggagc
    50221 caggcgaaac cacattacag cgcacgccgc tacccgccag ttccagcccg acgctcaacg
    50281 ccaggctttt cagcgccgct ttcgatgcgc cataagcact cataccaata cgcggcgtgt
    50341 gtgcggcgtc ggacgccaca gtgacaatcg ccccgccccg ctgacggcga aactggttca
    50401 tggtttgctg gaacaggtta aacgcaccgc cgacgttaac cgcaaaagtc tgctgccagt
    50461 cctctttact gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca ttgatcagca
    50521 cgtccagtcg ctccgtttca gctaacagtc gctgacatac ctgcgccacc tgcccggcgt
    50581 cggcaacatc cattacttcg gtcgcaaagg gatattgctc ctgagtgaac gcttgatcaa
    50641 aacctgtaac tttcgctccc gcctcaacaa acgccagcgc cgtggcgtag ccgatacctt
    50701 tacctgcgcc ggttacccag acatttttac cgctgaaatc cattatttca cctcgcggga
    50761 gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc agcatgacaa agtcgatatc
    50821 accatgcact ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat ccagaccgta
    50881 gtcgatcagg ttgtcgtcat cgaacggttc atcggactcg tccagcaacg gcaggataac
    50941 ctcacgcagc gccgctttgc tggcggggac aggtgctggc agtaattctt cagtcatcac
    51001 cacccggcca gaccgcccgg ccacatattt cagcgacatc aaatgctcgt cacggctgaa
    51061 atcggccagc gcatccgcca ccataaacgg tttaatatcg cgcataaatg cgtcggttgc
    51121 ggtggtcata cagccaatgt gggcatatac cccggtaata atcagctggt tacgtccact
    51181 ctctttgagc atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc acttcaccag
    51241 caccgtgtcg tcggcatctg gcgtcaggcg atccaccacc ttttgctgtt ccggcgaacg
    51301 ggtgaggccc ggcccccaca tatcattcaa cagcgcccga tcttcatcgc tctgctcttt
    51361 cggctgggcg gtgtaataaa ccgggatatt gtgctgtttg cagtagtcgc gcagcgcagc
    51421 aatattcgcg atcacctgct ccatcatcgg gcagttctcg ccccagaagc tgacaaaata
    51481 gtcctgcata tcatggatta acaacgcggc acgttgcggt tcaaaggccc agtcgacttt
    51541 attcttaggg atatcgtgag actccggcag tgcgtaagcc tgtaattttg gaatagccat
    51601 cgtgttctct ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat tgttttttat
    51661 cgactttccc gaccgccgtc agcggaagtg aatctacaca ctccacgcga tccggtaatt
    51721 taaattcggc aataccctgt tcacgcagga aacgacgcac ctgcaccgcg cgcagcggtt
    51781 ctttcaccac cagataagcg cagctttttt cgcccatcag ctcatcttcc atgctcacca
    51841 gtgcggcgta gatcaccgcc gggtggcgca gcagcaggtt ttcgatctct tcggcagcga
    51901 tcttctcacc gccacggttg atctgatctt tctcgcgccc ctgcacggtg atgtacccct
    51961 ctggatcaat agagatcaga tcgccggaac agtaaaaacc gttggcatca aaggcactgg
    52021 cattgtgctg cggacttttg taataaccgc ggaaggtgta cggcccgcgc gtcatcaggc
    52081 gtccaacttc cccttgcggc agtggatttc cttcagcatc agcaacccat acttcatcgt
    52141 ccggacacat tgggtaaccc tgggtatgga taattttctc cgcgctatca tcaagtcggg
    52201 tgtagttcac cagcccttcc gccatgccaa acacctgctg caacaggcag ccaatctcag
    52261 cgggaatacg cgccgcaagg gtggctgaaa gacgtgcgcc gccgacctgt aacagtttca
    52321 gcgaggcaag ctgcgcccgg ctttcacctt cggtcagcgc ctgcaaccac aggctgactg
    52381 ccggtggcac cagcgcggtg acgttcacct gatgtttttc aatcaatggg aagcaaagcg
    52441 tggcgctggg atcggccgcc agaacaaccg ttcctccggc aagaaagacg cccagcgatc
    52501 ccggcgaact catggcgtag ttatgagccg ccgggatcgc gcacaggtag cgtgtctgtt
    52561 gtgtgaactg acaaatctcg acgctacgac gcacgctgta gtagtagtcg ttatgagtgc
    52621 gcgggatcag tttcggtgtg ccggtggtgc cgccggaaag ctggaaatag gccacttcat
    52681 cagcaggtga tggcgtggca gtaaaatcct cagccggatg gttaatcgca tcctgcaagt
    52741 tatgctcacc gctgtcgttg tgcagttgca ccacgcgaat ggaggaatgt tctgtgacga
    52801 aagtattgag gaaatcatcc ccgctaaaca gcgcatgttg gcgatcggca atcagcaatg
    52861 cgggttcaat ctggctggca taggcgttca gttcactacg ctgatggctg aacagcgcca
    52921 gcaccggcgc aacgcccagt ttcagcagcg cgaaaaaggt aatatacaat tcagcgacgt
    52981 tacccagttg taccagcgcg gtttcaccag gtttaatgcc ctgacggcgt aaactacacg
    53041 cgaggttatc cgccgcctga ttcagctccc ggtaactcaa ctgtcgctcg ccgtcgataa
    53101 ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc ggtcagcggc aaatcctgcc
    53161 agtagccttt ttcccgatag cgacgggcaa actcttccgg ccagcgggtg aatggaatgc
    53221 tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt ttgacgcctg
    53281 tttcgcgcca ctcacccaac ggtgacgacg caggcacaat ccccgctccg gcaaacagac
    53341 gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg ccgttacctt
    53401 cgctgtcaca ccaacccaca atgccgccaa acagttcgcg gtcgaatggc tccagttcag
    53461 caataacctg ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtt ggatgcagca
    53521 gacaggccag agtcagtgcg ttttcttgcg aattcgcttt accttcaaag ggagttgcga
    53581 gatgccacag cgtcggcgtg gtaatcaatt gtggagagga gggaacgtgt aactcactac
    53641 tgcgttcgcg cagtacctct ttcatcgcct gagtcaccag ttcatgttca tggcgatctt
    53701 tttctgacgc cagcagacga ttacccgctt cgcgatcgag cacttcatcc ggctgacgac
    53761 gcgcggaacc ggctaacgga atggagctaa aacgctcgcc gtctttacgt agcagcagtt
    53821 ccgggctggc ccccagcagg acgccaccat cagccagcgg gacatggaag ttgtaactaa
    53881 ccgggttttg cgcaatcaac cgttccagca atacgccact atcaatggcg gcgtcagtgg
    53941 tgatatcaat caatcgtgac aacaccactt tgtcgacctg cggcgtggcg gtaagtgcgg
    54001 cggcgcgagc aaccatctgt tcaaacgtgg tttgttcagg aattgcctgg cgttccacca
    54061 cgttcagcga ctggctgcgg gtaaaacggc gggctgaggt ctgtttttcc tggcgggaga
    54121 acgactgcca ggattcggga atatacagcg acgaaggctg acgtggatcg aagggaatcg
    54181 ccccgaccat caccggattt ttgatgccct gcgctttggc atcggcaaac agcgcggcga
    54241 gtttttgctg gaagggactg tcgggcgaat ccccgttcac agccggttca tcgaagcggg
    54301 cgaaacatcc tgacgtcgta aaactgcggt acggcgacat aaagaaaaag cgattgggcg
    54361 caagtgttgc catggtctgc tgtacttcct cagccagtga cgtatccata tcatcctcca
    54421 caaaatgata aaggctttaa taatgattat catttatatt ttcggtcgct aacctaaaag
    54481 caaacgcgca ctatgtcaac tcttgaggta acgcaatctg caactcgggg ttgcatctgg
    54541 ttgtggttac aatgaaaatg agaagcatta ttgatggatt cgcataagcg caatgtgatg
    54601 tcctgcgccg ttctgccccc tctcccttcc agggtgaggg ctggggtgag ggttaatgtt
    54661 cgcaccagtg ctggctgttc ccctcaccct aaccctctcc ccaaaggggc gaggggacgg
    54721 attgtgcgct ttgtcgaatt tgtcattacg cccttaacct tattaataac aggaagctga
    54781 tttgtgagac tcgccccgct ctaccgcaac gcccttctat taacaggact tttgctttca
    54841 ggaatagccg cagctcaggc cgctgactgg ccgcgtcaga ttactgacag ccgtggcaca
    54901 catacactgg aaagccagcc gcagcgtatt gtttccacca gcgtcaccct gaccggctca
    54961 ctgctggcga ttgatgctcc ggtgatcgcc agcggcgcga ccacgccgaa taaccgcgtc
    55021 gcggatgacc agggcttttt acgccagtgg agcaaggtgg cgaaagaacg caaactgcaa
    55081 cggctctata tcggcgaacc gagcgccgaa gccgttgccg cgcaaatgcc ggatctgatt
    55141 ttaattagcg caaccggcgg ggattcggcg ctggcactgt atgatcagct ttccaccatc
    55201 gccccgacat taatcatcaa ttacgacgac aaaagctggc agtcgctgtt aacgcagctt
    55261 ggcgaaatta ccgggcatga gaaacaagcg gcagagcgga ttgcacagtt tgataagcaa
    55321 ctggcagcgg cgaaagagca aatcaaatta ccgccgcagc cggtcactgc cattgtctat
    55381 accgccgctg cacacagtgc caatctctgg acgccggaat cagcacaagg gcagatgctg
    55441 gaacaactcg gctttacgct ggcgaagttg cccgcagggt taaacgccag ccaaagccag
    55501 ggcaaacgcc atgacatcat tcagcttggt ggggaaaatc tggctgctgg gttaaatggc
    55561 gagtcactat tccttttcgc cggtgatcag aaagatgccg atgcgattta tgccaatcca
    55621 ctgctcgcgc acctgcccgc agtacaaaac aagcaggttt atgcgctggg aaccgagacg
    55681 ttccgcctgg attactacag cgccatgcaa gtgctggaac gacttaaggc gctgttttaa
    55741 gcattaactg tcagacgctg tcacctgcgg cggcgtctgg cgaaaacgtc gcaactccac
    55801 cagcaccagc aataacaaca cgccgataat caacaaacca aaaccgctcg cgcttgcgga
    55861 agcaaccggt gtcatcatcg cgcccaaacc acccagcagc gccgcgccta tggcatcgcc
    55921 cgtcacgttc tgcgccgtcc acaaaccgtt aatccgccct aacatcgctt ccggggtttg
    55981 cgtttgcagc attgtatatt gcagcaacga actaaccgcg ctcagccagc cgaacagcgc
    56041 cagacaaacc acgcctaaaa tccacatcgg catcaggcca aacagaccaa tggcgaggaa
    56101 cgatcccagc gtggagagca gcatcaataa ccctggtcgc gcactatgtg ccagcttccc
    56161 gctggttaac gcaccaatag ccgcgccgag cgggatcgcc gcgtagagaa aaccaatctg
    56221 tgccgctgac atctgccagt tgtcagccag cgccggatac agtacccgca ccgcgctcgc
    56281 catcgtcaat aaaccaccca gcagcgcaat ccctcctacc agcgggctgg cgagcagaaa
    56341 acgaaatcct gccagtaatg atttcaacgg atgctcgcgc ggctgcggtg gcggtggcaa
    56401 cgccggaagg cttaacaacg gtagcaaggt aataaacgtg cccgccgccg ccagcccgta
    56461 gttccaggct acgccgccgg tcgccagcaa taaaccgcca atcatgggcg aaatcaccga
    56521 ccccagacgc acggtcaaca tggtgatcgc cccggcctgc attaagtttt cacgccctac
    56581 cagtgcaggt gttgccgcca gtagcgccgt aacaccaagt gatgcgaaaa aaccatccca
    56641 taaaccaagt aaatagattg ccagcaatga cggctccggc agcagcgcgt tcaggcacag
    56701 tccaatgaag ccaatgccac aggtgccgcg cgccagcaaa atcacttttt tgcgttcata
    56761 gcgatccgcc agcacaccgc caaccatcag gccaacaaac atcgcgccgc cggtcagcgt
    56821 caccgaaagc cccacctgcc aggtagaatg cgtcatcatc tggatctgca ccggcaccgc
    56881 gacgccgagc aaacccagag acacaattga gataaaacga gcgaggaata ctgcgcgaaa
    56941 cgccgggtgc gttttcaaca ggctgaggtt aagcagccag gattgtttat tcattacaat
    57001 gccttgccat caaatatttt aaatacctat tcttaggctg cacatgctaa catatccaaa
    57061 taagatcgat aacgataatt aatttcatta tcatggaagt tcgtatgtct ggttctgttg
    57121 ccgtgacacg cgccattgcc gtgcccggat tgctgttatt actgattatc gcgacggcat
    57181 taagcctgct cattggggca aaatcactcc ccgcttccgt agtgctggag gccctctccg
    57241 gcacctgcca gagcgccgac tgcaccatcg tgctcgacgc gcgactgccg cgcacccttg
    57301 ccggtttact tgcaggcggc gcgcttggcc ttgccggggc gttgatgcaa accctcaccc
    57361 gaaatccact tgccgacccc ggcttgcttg gcgtgaatgc cggagccagc tttgccattg
    57421 tgctgggtgc ggcgctgttt ggttactcat ccgcgcagga acaactggcg atggccttcg
    57481 ccggggcgct ggtggcctca ttgattgttg cctttaccgg cagtcagggc ggcgggcagt
    57541 taagtccggt gcgtttaacc ctggcgggcg tggcgctggc ggcggtgctg gaaggactga
    57601 ccagcggcat cgccctgctt aatcctgacg tctacgatca actgcgtttc tggcaagccg
    57661 gttcgctgga tattcgcaat ctacatacct taaaagtggt gctgatcccg gttctgatcg
    57721 ccggagcaac tgcgctatta ctgagtcgcg cgctgaacag tttgagcctc ggcagtgaca
    57781 ccgcgacggc gctgggcagt cgcgtggcgc gcacacagtt aattggtctg ctggcgatta
    57841 ccgtgctttg tggtagtgcg acagcggtag ttggcccgat tgcctttatt ggcctgatga
    57901 tgccgcatat ggcgcgttgg ctggtgggtg ccgatcatcg ctggtcgctg cccgtcacgc
    57961 tactcgccac ccctgccctg ctgctgtttg ccgatatcat cgggcgggtg attgtgcccg
    58021 gcgagttgcg cgtttctgtg gtcagtgcgt ttattggtgc accggtgctg atcttcctcg
    58081 tgcgacgtaa aacgcgaggt ggtgcatgat ttacgtctct cgccgattac tcatcacctg
    58141 tttgctgctt atttccgcct gtgtggttgc aggtatctgg ggattacgca gcggtgccgt
    58201 cacgctggaa acctcgcagg tattcgccgc gctgatgggc gatgcgccgc gcagtatgac
    58261 gatggtggtc accgaatggc gtttaccacg cgtgctgatg gcgctgttga ttggcgcggc
    58321 actgggcgtc agcggtgcga tttttcagtc gctgatgcgt aacccgctcg gcagccctga
    58381 cgtaatgggc tttaacaccg gggcgtggag cggcgtgctg gtggcgatgg tgctgtttgg
    58441 tcaggacctg acggctatcg cgctggcagc aatggtgggc ggcattgtca cttctctgtt
    58501 ggtctggctg ctcgcctggc gtaacggtat cgacaccttt cggttgatta ttatcggcat
    58561 cggcgttcgc gccatgctgg tggcctttaa tacctggctg ttgctgaaag cgtctttaga
    58621 aacggcgcta acagcgggtt tgtggaatgc cggatcgctc aatggcctga cgtgggcaaa
    58681 aacttcgcct tccgcaccca tcattatatt gatgctcatt gccgccgcct tgctggtgcg
    58741 gcgaatgcgc ttgctggaga tgggcgatga taccgcctgc gcgctgggcg tcagcgtcga
    58801 acgttcgcgt ctgttaatga tgctggttgc agtggtgctt accgctgcgg caacagcgct
    58861 tgccgggccg atttccttta ttgctttagt cgcgccgcac atttcccgac gcattagcgg
    58921 caccgctcgc tgggggctaa cccaggcggc gctatgcggg gcgctgttac tgctggcggc
    58981 cgatctctgc gcccaacaac tgtttatgcc gtatcaactt ccggttggcg tcgttaccgt
    59041 cagcctcggc ggtatttacc ttatcgtctt gttaattcag gagtctcgca aaaaatgacc
    59101 gaatcagtag cccgtttgcg cggcgaacag ttaaccctgg gatatggcaa atataccgtt
    59161 gcggaaaatc tgactgtaga aattcctgat ggtcacttca cggcaattat cggaccaaat
    59221 ggctgtggta aatccacgtt actgcgtacc ttaagccgcc tgatgacgcc tgctcacggg
    59281 catgtctggc tggatggcga gcacattcaa cattacgcca gtaaagaagt tgcacgccgg
    59341 attggtctgt tggcgcaaaa tgctaccacg ccgggcgata tcaccgtgca ggagctggtg
    59401 gcgcgtggac gttatccgca tcaaccgctg tttacccgct ggcgcaaaga ggatgaaaaa
    59461 gcggtaacga aagcgatgca ggccacggga ataactcatc tggcagatca aagcgtggat
    59521 accctttctg gcggacaacg ccagcgagcg tggatcgcga tggtgctggc ccaggaaaca
    59581 gcaattatgc tgctcgacga acccacgacc tggctggata tcagtcatca gattgatcta
    59641 ctggaactgt taagcgaact gaaccgcgag aaaggctata ccctggcggc ggtgctgcac
    59701 gatcttaatc aggcctgtcg ttacgccagc catttgattg cattgcggga agggaaaatt
    59761 gttgctcagg gagcgccgaa ggagattgtc actgctgaac tgattgagcg catttatggt
    59821 ctgcgatgca tgatcattga cgatccggta gccggaacgc cgcttgtggt gccgctcgga
    59881 cgaacggcac cttcaaccgc aaatagttaa actaagtggt ctgccatcat ggcatcctgt
    59941 tttctggatg ccatcgcata gcgcaataac acgctaccac aagccaccat cccgccgatc
    60001 agcgcggaaa ggatcacaat aatcgcctta cccggaccgt cttttttcac tggcaatgac
    60061 gggcttaact gatatttaaa cggcgtaaaa ttcacatcgt tgatatttgc ttttgttaac
    60121 tgctcaacaa gatactgacg attacgtaat tcaccgttca gttccgcaac gtcagtgacc
    60181 gctttttcaa tttccagttt gcgttcaata ccgtctgcgc cgagagaaat agagaaatcg
    60241 gggtcatctt taacggcctg accattactg tatacgggct ttttaattcc cgccgcgttg
    60301 gcaatgtcga gtgaataatt gaggcgctga atgtttgcat caagttgatt tttcgtttta
    60361 atgcgatcct gagccagttt ttctttttca aactgggttt tgatctccag tttattacgg
    60421 acgttttcta tcgactcttt caccaccaac gcagagatat aatcgatata cccgctcaaa
    60481 acggtctgcg cctctttact ggttggcgcg gtaaaactta gcgtccagga ggtatacagt
    60541 gacggttcat cttttttctt actggcattg tcatcaaccg ctttcatttt ttcgctcaat
    60601 gcaacaatag cgcgatgcaa atccagttca tcgattttcg cctcttttaa ttggtccatc
    60661 acataaggtg atgaacgcag gtactcttcc agcaagctaa cagactgaaa cttcttgata
    60721 aacaggttaa atgcaccgcc acggtcgata ttaacatcga gatcaagaac gcggagtttg
    60781 gtaaaggttt tctccaggtc ctgccactga atggcttcgg cgggagtaat aacggctgag
    60841 ctggtccatt tttgcggcag gatgaaagag atcagtaaac ctgcgcaggc aaatgcaaaa
    60901 acgaccgcca tgaccgtttt tttggcccgc cataaaactt cgattagatt aagtaaatca
    60961 atttcattat tactgggcga cgccagagga tactcagaaa aatgagcgtc acttccctgt
    61021 ttaatattca atgatgacat ggggtataac ctgaaaatga gtccaatagg ggaaaatctc
    61081 cacggcgctc actttaccag ttggtgagaa ttatccgaag ctgaagtttg taaattcctt
    61141 ccactgaatg caacattcac tctatttaag agaaataact agcaaactta taaactaaat
    61201 aaatgattaa cccataatta tttcctataa ttatgggtta atagataatg ttaatttacc
    61261 tgtttagcgt tgcgcgaata atcggcccaa ttttttcaaa cgcctctgaa gatataatat
    61321 ccacatgcgc acaatcctga cgataaatat ccagctcggc tatccacggc gaccaggcgc
    61381 gttcgggact cataccttcc tgaagcgtgc gttcagcaac aaacagcgtc gctttaccgt
    61441 caaacggtac gctatgagca gtcgtcagca ggcgcactgc atcagcgtag ttgccttcaa
    61501 tggtggtaaa caactccgtt gaagtacctc cctgctgtgc cgccaggaag gcctcgcgtt
    61561 cgcggttaat ctccgccagc acttccgggt ccagaccatt agcttctttt tcctgccagt
    61621 tttgcgtttc tggcggccag gtatccagca agccaagaaa tgccacctgt tcgccacggg
    61681 cacgcagtcg cgccgcaata ccctgcacca gcgtaccgcc aagggaatac cccagcaggt
    61741 aataagggcc gtgcggttgt tgctcaagta acgttgccag atgcgcttcg cagacttcat
    61801 ccaggtttgt cgctgtctgc atgggtccat gagggcgcgg cgactgaatg ccaataatcg
    61861 accattgtgg atcgagataa cgcgagagca cgctaaactg ccaggcaaaa ccggatgccg
    61921 gatggaaaca aaacagcgtc gggccattac cttcacgcaa cggcagaatg gtttcgaatc
    61981 ccatgcgccg ggagctgtcc tcttcaccat caataatcgt tgccagtttg gcgacggttg
    62041 acgcgaccat cacctgcccc ggcgtcacct gacgagcaaa ctgtcgactt aactgcgctg
    62101 ccagtttcat tgccagtagc gaatgaccgc caagcgcaaa gaaatcagca tcggcatcct
    62161 gcacgtcaca acccagcaac gacgcgaatg ccgcggcgat aatcgtttca ctgcccgctt
    62221 tcggtgcacg tcccgacgct tgtgtcttca gttcaggcaa cggtaaggct ttgcgatcca
    62281 gcttgccgtt ggcgctaaga ggtaactgtg gaagttgcag cagaaccacc ggcaccatat
    62341 gcggcggcaa tgtttcgcga agctgtgcct gtaatgcgct ggtatccaac ggcagacctg
    62401 attgcgacac cagatagccc accaactgac gcgcatcacc accggtggct gccgccaggt
    62461 taatcacaca ggcgtgggta acggcttgtt tgacatccgg cagcgcctgc atcacgcgat
    62521 cgatttcgcc cagttcgata cgctgcccgc gaatttttag ctgatcgtca ctgcgcccga
    62581 ggtactccac cgcgccgtta tccagccagc gggcgacgtc tccggtacga tacatccgtt
    62641 cacctggggc aaaaggatcg gcaataaaac ggctggcggt cagatcgggt cgtccaagat
    62701 acccctgcgc cagttggatg ccggtgagat agagatcacc cgccacaccc ggcggcaccg
    62761 gatgcatcat cgcatcaaga atacgcagac ccgtattcca taccggataa ccaatcggca
    62821 cactgctgcc gcgcacctgt gccagttcct cgccaaaagc cggataccag ctgacatcta
    62881 ccgccgcttc cgtcgggcca tacaggttat gcaacggcgc gccggttaac tgttgccatt
    62941 cgcggcataa atcagccggt aaggcctcac cactacagaa aacctgtttc aacgtcgcgc
    63001 aattctggcg agcggtttgc ggcgtcagcg atgcaacaaa tgccgccagc atcgacggca
    63061 caaagtgcgt ggtcgttacg ccatattcgg caaagaattg ctgcatagcg agcgggtcac
    63121 ggtgcgcttc cggttcagcc atcaccagtt ttgcccctgc gataaacggc cagaaaaact
    63181 cccacaccga gacatcaaaa ctgcacggcg ttttttgggc aacgacatct tcgcctgtaa
    63241 gcgggtagtg attttgcatc cataacaggc ggttaacgat agccgtctgc ccgaccatta
    63301 cccctttcgg cctgccggtg gagccagagg taaagatgat ataagccgtg tggtgcggtt
    63361 gtgaaagttg cagcggcgca ctgccctggg gggtaagcgg ggcgttatag caaaggcttg
    63421 ttaaattggg aacatcgcta aagcgcggca gttgatcgtc ggtggtaatt aacagcgacg
    63481 gacgcgcatc ttccagcatc attttcaggc gatcgtccgg atagccggta tccagcggta
    63541 gccaggccgc gccagcttca actattgcat ggagtgccag ggtcaaaaag accgagcgcg
    63601 gtagtgccac cgccacgctg tcccctggtt taacgccgcg ctcacgcagc agattcgcca
    63661 gcgccaccac ctgctcgcgc atttcccgat agctgaacag gtaacgcgca tctgccagcg
    63721 ccggagcatc cggtgttttt gccgcttgtt ctgccaccag cgcgctcagc gtggtttctg
    63781 gaatctcaac ctgagtggcg ttgatctgcg ccagctgcgc atactcacct ggcagcataa
    63841 tatcgacatc gccgcacaac agcgccggat ccgcggcgaa ctgggcaatc agcattttca
    63901 ggcgttcagc atgctggatt aacgttggct catcgtaacg ctgtttattg gcgaggatct
    63961 caatactcaa atcaccgtgt tcatccggga acagggccag ttcaaggtca ttaaccggac
    64021 cggttgccag ggtatgggtt tgcgtctgaa caccaggaat atccagttgg taatcaaata
    64081 ccttgatatt gagtaccgga ccgaacagcg gttcctctcc tgccgctcgc ccgctgtcac
    64141 ggacaatttg ttcggcatcg taacgttgat gacgacgcat ttttttcagt tgtgctgcca
    64201 gtcgggttgc cagttccggc agcgtttctt gcgccgcaat gtgaataccc aacggtaaaa
    64261 cgttgagcac gggtccgcta gccgtcagcg ccgccgagcc cagtcgacgc ataaagataa
    64321 atccggcggc atagtccatg cgattgcaca atcgccccag ccacaaggct gccagcgcaa
    64381 gggctaaatc ggtacgctgc acacctgaaa gttgcgtagc cagctggcgg aattccccgt
    64441 cggtaaattc cagtttcagg cgcagaatat ctgccgaagc gctgcgcccc gctaaaggtg
    64501 ccggagaaag tgacgcgggc ggcggcagtt gacgacgctg ttctgcccag aatgccgcat
    64561 cacgctgcca ggcttcgctt tcgcggtatt gctggtactc ttccactaca tcagcgaaag
    64621 gcgtaaacgg cgaagcaggc gttggttcgc cacgcagcca tgtgcagtaa atattggcga
    64681 tctggcgagt aatggccggg aaactgaagc catcgaccag caaatggtga taacgctgat
    64741 accagtacca gcggttatcc gcaacatgta tcagctgatg aaagaccagt ggtttaccgc
    64801 tatcgacgcg caaatcttgt tgcaaatccg ccagcattaa tgcctgcgca gtaccgtgcg
    64861 gatcaatatt ggttcgcagg tcgataattt ctggcagttc gaatatcagc gcatcatcga
    64921 cccactgcca gacttcgccg ttatcttccg taaaacgcat ccgcaacgta tctgcttgcg
    64981 ctagtccgac aaccaccgcg cgggccagta atggcgcatc aacatctcca gttaactcaa
    65041 cgtaatgcgc cacgctccag gcggagggta aatccgacag tttttctgcc atccagatgc
    65101 cgggctgtgc ggcgaccaaa ggtaaatgct ggctcattgt gcctcctgca actgggtgaa
    65161 attggccggt gtcagagtac gccagtggac ttccagccac tgctggcagg acgcctgtga
    65221 ctgcggctga cacacaatgt cccagcctgc cggtaaggcg cattgttgcg gccacagact
    65281 gaattgcccc tgcgcattgc gcaaaatgta aaacgctccc tgtggatcat cgaagggatt
    65341 actgaatgcc atattcaact cctgtcatgg aaaagtggtt gccagaggtc gattagcccc
    65401 tgcatcaagc caccgcgcca acaaagcgca tcatgtccgc cgtcaacctg acgccagaaa
    65461 atggattctt ttatggggtg taattgcgca tacagcgcct gattggcccg cataatcatc
    65521 ggctcgcgaa tacccgcttc cagcacaatg cgcagacctt cggcgctaac ttcaccagct
    65581 ttaagctttt caagtaacac gccctcttgc tgcccgcccc gatgcggcca ccagtacgat
    65641 cctgactggc ttaatacaca gccaaagcgt tcaggccagt gcagtccggc atacagcgcg
    65701 gacagcccac caaaactctg cccggcgacc acggtgcgat cggcgcgatc gctaaaaggg
    65761 gcaatagctt tcaccagggg taataactct tgctgtactg ccagccagaa atccgcatta
    65821 cacggcagtt cgtgggcgcg gtgcgtggtg tcgatagcgt cgatcaacac atacacggcg
    65881 ggaggaagtt gctgacgatg ggtcagcgaa gtcagcacag gccagacggg catactttgc
    65941 gcccaaaatt cgccatcgag caaaactgcc agcggacgtt cttctgctgt tgcatcgccg
    66001 gtggtaaaaa tccatacacg acgtgaattt ttcaaccgtt cacttttcca gataatttct
    66061 ttggctgcta tttctggcgc ttgcggacaa tcccatcccg gttgcagagg cgcttgcggc
    66121 atttcgagtg cagaaacagc gtgccctcgc ccgcctttcc agctttgtgg attcagcgga
    66181 tcggctattg cctggggtaa tagttttcgc cagccttcac gtaattcgag gcgatcgggg
    66241 gatggtgcag aaaaaatgtc atcgcgttcg gtgggaataa agcagtagct gccgcgccag
    66301 ttggcattga gttgtgtcgt ccactgccag acatcagtac ctgcaattcg ctgcatcgac
    66361 tggggctggc tgttctgatg atgatcggtc acaccagtga tgtagatcca tacgcgcttt
    66421 atcgtcgagt attcttcaga accttgggga tcacgccacc agaaagtgac ctcaaacatt
    66481 tcgtcattca gacgctgcca ttccgggcca tgtttcgact gccaccagct ctcacttcct
    66541 acttttaacg ccgtcacacc ataaccccat gtttactgtg caatttttca ttgattgcag
    66601 aaatatattg ataatattat tgataactat ttgcatttgc aatagcgtaa tggcgcgccg
    66661 tgggaagcgc ggacatttat taaccaactg cactgcgtgt ctttcaggat caaaggtttt
    66721 cgcggtagcg ggatgcgtcg tgttgaggac gaccatgccc gacagttgca attcgtggca
    66781 aaaatgcagg aataaaacaa tgaacaagaa gattcattcc ctggccttgt tggtcaatct
    66841 ggggatttat ggggtagcgc aggcacaaga gccgaccgat actcctgttt cacatgacga
    66901 taccattgtc gttaccgccg ccgagcagaa cttacaggcg cctggcgttt cgaccatcac
    66961 cgcagatgaa atccgcaaaa accccgttgc ccgcgatgtg tcggagatca tccgtaccat
    67021 gccaggcgtt aacctgaccg gtaactccac cagtggtcag cgtgggaata accgacagat
    67081 tgatattcgc ggtatgggtc cggaaaacac gctgattttg attgacggca agccggtaag
    67141 cagccgtaac tcggtgcgtc agggctggcg tggcgagcgc gatacccgtg gtgatacctc
    67201 ctgggtgcca cctgaaatga ttgaacgtat tgaagttctg cgtggtccgg cagctgcgcg
    67261 ttatggcaac ggcgcggcgg gcggcgtggt taacatcatt accaaaaaag gcagcggtga
    67321 gtggcacggc tcttgggacg cttatttcaa tgcaccagaa cataaagagg aaggtgccac
    67381 taaacgcacc aacttcagcc tgaccggtcc gctgggcgac gaattcagct tccgcttgta
    67441 tggcaacctc gacaaaaccc aggctgacgc gtgggatatc aaccagggcc atcagtccgc
    67501 gcgtgccgga acgtatgcca cgacgttacc agccgggcgc gaaggggtga tcaataaaga
    67561 tattaacggc gtggtgcgct gggacttcgc accattgcaa tcgctggaac tggaagcggg
    67621 ttacagccgc cagggtaacc tgtatgcggg cgatacccag aacaccaact ctgacgctta
    67681 cactcgctcg aaatatggcg atgaaaccaa ccgcctgtat cgccagaact acgcgctgac
    67741 ctggaacggt ggctgggata acggcgtgac caccagcaac tgggtgcagt atgaacacac
    67801 ccgtaactcg cgtattccgg aaggtctggc gggcggtacc gaagggaaat ttaacgaaaa
    67861 agcgacacag gatttcgtcg atatcgatct cgatgacgtg atgctgcaca gcgaagttaa
    67921 cctgccgatt gatttcctcg ttaaccagac gctgacgctg ggtacggagt ggaatcagca
    67981 acggatgaag gacttgagct ccaataccca ggcgctgacc ggggcgaaca ccggcggcgc
    68041 tattgatggt gtgagtgcca ccgaccgtag cccgtattca aaagcagaaa ttttctcgct
    68101 gtttgccgaa aacaatatgg agctgactga cagcaccatc gtaacgccgg ggctgcgttt
    68161 cgatcatcac agtattgtcg gcaataactg gagcccggcg ctgaacatat cgcaaggttt
    68221 aggcgatgac ttcacgctca aaatgggcat tgcccgtgct tataaagcac cgagcctgta
    68281 ccagactaac ccgaactaca ttctctacag taaaggtcag agctgctatg ccagcgcggg
    68341 cggctgctat ctgcaaggta acgatgacct gaaagcagaa accagcatca acaaagagat
    68401 tggtctggag ttcaaacgcg acggctggct ggcgggcgtc acctggttcc gtaacgatta
    68461 tcgcaataag attgaagcgg gctatgtggc tgtagggcaa aacgcagtcg gcaccgatct
    68521 ctatcagtgg gataacgtgc cgaaagcagt ggttgaaggt ctggaaggat cgttaaacgt
    68581 accagttagc gaaacggtga tgtggaccaa taacatcact tatatgctga agagtgaaaa
    68641 caaaaccacg ggcgaccgtt tgtcgatcat cccggagtac acgttgaact caacgctgag
    68701 ctggcaggcg cgggaagatt tgtcgatgca aacgaccttc acctggtacg gtaagcagca
    68761 gccgaaaaaa tacaactaca aaggtcagcc agcggttggc ccggaaacca aagaaatcag
    68821 tccttacagc attgtgggtc tgagcgcgac ctgggatgtg acgaagaatg tcagtctgac
    68881 cggcggcgtg gacaacctgt tcgacaaacg tttgtggcgt gcgggtaatg cccagaccac
    68941 gggcgatctg gcaggggcca actatatcgc gggtgccggg gcgtatacct ataacgagcc
    69001 gggacgtacg tggtacatga gcgtaaacac tcacttctga tgctaacgtc agattgttga
    69061 caaagtgcgc gtcgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaaatc
    69121 ttgccaattc aatatattgc aggaccatgt aggcctgata agcgtagcgc atcaggcagt
    69181 ttagcgtttg tcatcagtct ctaatatggt cgacatgaaa actacgcata cctccctccc
    69241 ctttgccgga catacgctgc attttgttga gttcgatccg gcgaattttt gtgagcagga
    69301 tttactctgg ctgccgcatt acgcgcaact gcaacacgct ggacggaagc gcaaagccga
    69361 acatttagcc gggcggatcg ctgcaattta tgcgctgcgg gaatatggct ataaatgtgt
    69421 gcccgcaatc ggcgagctac gccaaccggt gtggcctgcg gaggtatacg gcagtattag
    69481 ccactgcggg gctacggcat tggccgtggt ttcccgtcaa ccgattggcg ttgatatcga
    69541 agaaattttt tctgcgcaaa cagcgaccga attgacagac aacattatta caccagcaga
    69601 acacgagcga ctcgcagact gcggtttagc cttttctctg gcgctgacac tggcattttc
    69661 cgccaaagag agcgcattta aggcaagtga gatccaaact gatgcaggtt ttctggacta
    69721 tcagataatt agctggaata aacagcaggt catcattcat cgtgagaatg agatgtttgc
    69781 tgtgcactgg cagataaaag aaaaaatagt cataactctg tgccaacacg attaattgac
    69841 aacatctggt acgattcgcc cgcagccatc actgaccgcg ggcgaaagtg taaagcaggt
    69901 gccttaccac tctgacctga caaccggata tgcggggatt gctccccgca caacggctac
    69961 ttcttcggtt cgtaagcgag tacagcctta aactcaatat tacgttcctt tacggtgaac
    70021 tcacacagcg agtctccgac cagaagcgta aatcccagta ccgtcaaaca cagcactatg
    70081 actgccacaa gggcatattt cgtcagcatg tttatattgc ctccatgcga agaagggact
    70141 accatccaaa ttgttcaggt ttgggtaggt agccccaggg ttgatagaaa tatcgtcctg
    70201 gggcttcatc tttccagacc tcagagtaaa cctgaaaccg gaaagcttca ggcacccgca
    70261 gttatcttac ccttgcttag caaaatagct gtttatttat acagcaagtc ctgtttcgga
    70321 cttctcactc cctatttgat caactgaagc tgaacccgtt tttttctgca tgcctcaatc
    70381 gtcaaaattc ttcgccacca gccagccgtg ccataacttg tcacattgca aaagtagcca
    70441 aattgcttca gtcaccatca ccaaaaaaag aaacattcca gaacttccgc atctcgccca
    70501 gccgctccga cttctatact gaatagaaaa cgccaactta agagaaacct atgccattac
    70561 ccgattttca tgtttctgaa ccttttaccc tcggtattga actggaaatg caggtggtta
    70621 atccgccggg ctatgactta agccaggact cttcaatgct gattgacgcg gttaaaaata
    70681 agatcacggc cggagaggta aagcacgata tcaccgaaag tatgctggag ctggcgacgg
    70741 atgtttgccg tgatatcaac caggctgccg ggcaattttc agcgatgcag aaagtcgtat
    70801 tgcaggcagc cgcagaccat catctggaaa tttgcggcgg tggcacacac ccgtttcaga
    70861 aatggcagcg acaggaggta tgcgataacg agcgctatca acgaacgctg gaaaactttg
    70921 gttatctcat tcaacaggcg accgtttttg gtcagcatgt ccatgttggc tgcgccagtg
    70981 gcgatgacgc catttatttg ctgcacggcc tgtcacggtt tgtgccgcac ttcatcgccc
    71041 tttctgccgc gtcgccgtat atgcagggaa cggatacgcg ttttacctcc tcacgaccga
    71101 atattttttc cgcctttcct gataatggcc cgatgccgtg ggtcagtaac tggcaacaat
    71161 ttgaagccct gtttcgctgt ctgagttaca ccacgatgat cgacagcatt aaagatctgc
    71221 actgggatat tcgccccagt cctcattttg gcacggtgga ggttcgggtg atggataccc
    71281 cgttaaccct tagccacgcg gtaaatatgg cgggattaat tcaggctacc gcccactggt
    71341 tactgacaga acgcccgttc aaacatcagg agaaagatta cctgctgtat aaattcaacc
    71401 gtttccaggc ctgtcgctat gggctggaag gcgtcattac cgatccgcac actggcgatc
    71461 gtcgaccgct aacggaagac accttgcgat tgctggaaaa aatcgcccct tccgcacata
    71521 aaattggtgc atcgagcgcg attgaggccc tgcatcgcca ggtcgtcagc ggtctgaatg
    71581 aagcgcagct gatgcgcgat ttcgtcgccg atggtggctc gctgattggg ctggtgaaaa
    71641 agcattgtga gatctgggcc ggtgactaag tagggaattg ccttacagaa gaatatccct
    71701 gacaatggtg ttttaactta catttaacaa tgttatgaaa caccctttag aaaccttgac
    71761 taccgcagca ggcattttgc tgatggcttt cctctcttgc ctgctgctgc ccgcccccgc
    71821 actggggctg acgctggcac aaaaactggt gaccatgttc catctgatgg atcttagcca
    71881 gctttacact ttattgtttt gtctgtggtt tttagtgctg ggcgctattg agtattttgt
    71941 tctgcgcttt atctggcgac gctggttctc gctggcggat taaacgtcga ttatcgccgt
    72001 acttcgcggc atactttgct tattctcctt cgcctaaagg aacgtttatg gataagcaat
    72061 cactgcacga aacggcgaaa cgcctggccc ttgagttacc ctttgtcgag ctttgctggc
    72121 cttttggccc ggagttcgat gtttttaaaa ttggcggcaa aatttttatg ctgtcgtcgg
    72181 agctgcgcgg cgtccccttt atcaatctga agtccgatcc acaaaaatcc ctgttaaatc
    72241 agcagatata cccgagcatt aagccagggt atcacatgaa taaaaagcac tggatttcgg
    72301 tgtatcccgg cgaggaaatc tccgaagcgt tacttcgcga tctgatcaac gattcgtgga
    72361 atctggtggt tgatggtctg gctaaacgcg atcaaaaaag agtgcgtcca ggctaaagcg
    72421 gaaatctata gcgcattttt ctcgcttacc atttctcgtt gaaccttgta atctgctggc
    72481 acgcaaaatt actttcacat ggagtcttta tggatatcat ttctgtcgcc ttaaagcgtc
    72541 attccactaa ggcatttgat gccagtaaaa aacttacccc ggaacaggcc gagcagatca
    72601 aaactctcct gcaatacagc ccatccagca ccaactccca gccgtggcat tttattgttg
    72661 ccagcacgga agaaggtaaa gcgcgtgttg ccaaatccgc tgccggtaat tatgtgttca
    72721 acgaacgcaa aatacttgat gcctcgcacg tcgtggtgtt ctgtgcaaaa accgcgatgg
    72781 acgatgcctg gctgaagctg gttgttgacc aggaagatgc tgatggccgc tttgccacgc
    72841 cggaagcgaa agccgcgaac gataaaggtc gcaagttctt cgctgatatg caccgtaaag
    72901 atctgcatga tgatgcagag tggatggcaa aacaggttta tctcaacgtc ggtaacttcc
    72961 tgctcggcgt ggcggcgctg ggtctggacg cggtacccat cgaaggtttt gacgccgcca
    73021 tcctcgatgc agaatttggt ctgaaagaga aaggctacac cagtctggtg gttgttccgg
    73081 taggtcatca cagcgttgaa gattttaacg ctacgctgcc gaaatcacgt ctgccgcaaa
    73141 acatcacctt aaccgaagtg taattctctc ttgccgggca tctgcccggc tatttcccct
    73201 cagattctcc tgatttgcat aaccctgttt cagccgtcat cataggctgc tgttgtataa
    73261 aggagacgtt atgcaggatt taatatccca ggttgaagat ttagcgggta ttgagatcga
    73321 tcacaccacc tcgatggtga tgattttcgg tattattttt ctgaccgccg tcgtggtgca
    73381 tattattttg cattgggtgg tactgcggac cttcgaaaaa cgtgccatcg ccagttcacg
    73441 gctttggttg caaatcatta cccagaataa actcttccac cgtttagctt ttaccctgca
    73501 ggggattatc gtcaatattc aggcggtatt ctggctgcaa aaaggcaccg aagcggcaga
    73561 tattctgact acctgcgcgc agttgtggat catgatgtat gcgctgcttt cagtcttctc
    73621 gttgctggat gttattttga atctggcgca gaaattcccg gcggcatctc agttaccgct
    73681 gaaagggata tttcagggga ttaaactgat cggcgcaatc ctggtcggca ttttgatgat
    73741 ctcgctgctg attggtcagt cgccagcgat tctgatcagc ggtcttggtg caatggctgc
    73801 cgtgctgatg ttggttttta aagatccgat tcttggtctg gtggcaggta ttcagctttc
    73861 cgcgaacgat atgctgaaac tgggcgactg gctggagatg ccgaaatacg gcgcggatgg
    73921 cgcggtgatc gatattgggt taaccaccgt caaagtgcgt aactgggaca ataccattac
    73981 cactattccc acctggtctc tggtttctga ctcctttaaa aactggagcg ggatgtcagc
    74041 atctggcggg cgacgtatta agcgcagtat cagtattgat gtcaccagta ttcgttttct
    74101 tgatgaagac gaaatgcaac gtctgaataa agcgcacttg ttaaaacctt atttaaccag
    74161 ccgccatcag gaaattaatg agtggaatcg ccagcaaggt tctacggagt cgatattaaa
    74221 cctgcgccga atgaccaata ttggaacctt tcgtgcctat ctgaacgaat atctgcgtaa
    74281 ccatccgcgg attcgtaaag atatgacttt aatggtacgc caactggctc caggtgataa
    74341 cggtttaccg ctcgagatct atgcgtttac caacaccgtg gtgtggctgg aatatgaaag
    74401 cattcaggct gatatattcg atcacatatt tgcgattgtc gaagagtttg gtctgcgact
    74461 tcatcagtcg ccaaccggca atgatattcg ctctctggcg ggtgcattta agcagtaatt
    74521 aaaaaaaccg ctctcatcga atggatgaga gcggacatcg ttttgtcgga tgtagcgtga
    74581 atgccttatt tccgacgcag cgttttaaat gccacaaaca ggaatacaat ccacaccggc
    74641 agcaggatcg ctgacaagcg catatcatcc atcgtgcaca tcagcagcaa aatcatgccg
    74701 aggaaggcaa tgcaaagata gttgccgaac ggatagagca gcgccttaaa ctgtgtttca
    74761 cgcccctgac gtcgcatcgc tgcacgaaaa cgcagatgcg ccaggcagat cataatccag
    74821 ttcaacagca gcgttgctac caccagcgcc atcagcagac caaacgcttt ttgcggcagc
    74881 agatagttga ttaataccac cagcgaagtg atcgctccgg aaagcatcag cgaattaatc
    74941 ggcacaccgc gacggctgac gcgagtcaaa aacttcggcg cattaccctg cacagaaagg
    75001 ccaaacagca tgcggctgtt agagtaaacc ccgctgttat acactgacag cgatgctacc
    75061 agaatgacga agttcagcgc agaagctacc acgttgctgt cgagattatg gaaaatcatc
    75121 acaaacgggc tactgttgga tttcacttcc acccacggat agagcgccag taaaaccacc
    75181 agtgagccga tgtaaaacag caggatgcga tacaccacct gatttaccgc ttttggaatg
    75241 cttttttccg gatcgcgcgc ttcagcggca gtaatcccaa tcagctccag accgccgaag
    75301 gagaacataa ttaccgccag cgacaaaatc agcccattcc agccggtggc aaagaaacca
    75361 ccgtagcgcc agaggttgtc gatactggct ttctcgccgc cgtgaccaga aaacagcagc
    75421 cacaggccaa agccgatcat accgatgatc gccagcactt taatcaacgc aaaccagaac
    75481 tcggtttcgc catataagcg cacgttcacc aggttaacgg cgttgatgat aataaagaag
    75541 gcggcagccc aaatccacgt tggaacatcc gggaaccagt actgcatata gatgcccgca
    75601 gcggtcagct ctgccatccc caccagcacg aacatcaccc agtagttcca gccagagagg
    75661 aagcccgcga acggtcccca gtatttatag gcaaagtggg caaacgaacc ggataccggc
    75721 tcttcgacca ccatctcgcc gagctggcgc ataatcagga aagcgatgat cccggcgacg
    75781 ccgtagccca gcaatacagc cggacccgcc atctgaatcg ccgggccaat gccaagaaac
    75841 agaccagtac caattgcgcc acccagcgca atcagttgaa tatgacggtt atgtaatccg
    75901 cgatgaagcg tcggctcttg attcgacgca gtatcttccg atacggttga cgcgtttttc
    75961 acgcctttcc cctgtgtgtc ttttttgttg aggggcacct tttaacattt agtgcccatc
    76021 gtcgcaagac acaatccaca cggttaaacg gggtatcctg cttttatttc cgtacccgat
    76081 gtcggtgcag ccacatcagc ttatacgccg ccgggataat aaacagcgac agcaaaggtg
    76141 cggtgatcat gccgccaatc atcggtgcgg caatccggct catcacctct gaccctgctc
    76201 ctgttcccca cagaatcggc agcagaccag cgataatcac cgccaccgtc atcgctttcg
    76261 ggcgcacgcg cagtaccgcg ccgtgataca acgcctcatc cagcttctgc tcgctgaatg
    76321 tttgcggatt attcaacgac ggctcggcct ctatggcgtg acgtaaatac atcagcatca
    76381 ccacgccaaa ttcggcggcg accccggcga gggcgataaa gccagtgccc gtcgccacgg
    76441 aaagatgaaa gcccatccac cacagcaacc agatgccgcc caccagcgca aacggtacgc
    76501 tgctgataat cagcaacgct tcgcccaccc gacggaacgc cagatacaac agcacgaaga
    76561 taatcatcaa cgtcatcggc accatgagtt taagcttatg gttggcgcgc tccagtagct
    76621 cgaactgccc ggagaatgcc acgctggtgc ccggttttaa ctgcactttt tcagctatcg
    76681 ctttttgcaa atcgtgaacc accgacacca tgtcacgatc gcgggcatcg atataaatcc
    76741 agctcgtcgg gcgcgcattc tcggttttca gcattgacgg tccggtagag actttaatgt
    76801 cggccacgtc tgccagggtg atttgctgct tcatcggcgt cagaatcggc agctggcgca
    76861 gtgcctgcgg actatcgcgc cagctttgcg gataacgcag attaattgga taacgggcaa
    76921 ttccttccac cgtttcgcca accatcgccc cgcccacagc agaagtcaca aacaactgca
    76981 catccgccac cgtcataccg taacgcgcgg ctttttcacg gttaatctca acgttgatat
    77041 agcgcccacc ttccagccgc tcggcaagag cagaagctac gcctggcacc gttcgcgcca
    77101 cttcttcaat ttgctcagcc atcgcgtcga tatccgccag cacagtgccg gaaactttaa
    77161 tgccgatggg gcttttaatg ccggttgaga gcatatcgat acggttacga attggcggca
    77221 cccacagatt cgccagcccc ggcagccgca cggtgttatc cagttcctca atgattttgt
    77281 ccatcgtcat gcctggccgc cactgctcct gcggcttaag ctggatggtc gtttctacca
    77341 tctccagcgg agcagaatcg gtggcggttt ccgctttccc ggttttgcca aatacccgcg
    77401 ccacttcagg tacgctcata attagcttgt cggttttttg cagcatactc gccgcctctg
    77461 ctgcggaaat ccccggcagc gtcgatggca tatacaacaa gtcgccttca ttgatctgcg
    77521 gtaaaaattc cccgccaact ttattgagcg gccagagaac cgtcagcacc gaaagcgccg
    77581 ccaccagcag cgtggttttc ggccagtgca gtactttcag caacagcgga tgataaacac
    77641 gaatcaaaaa gcgattgagc gggttactgc tttccggcgg aattttgcca cggatccagt
    77701 agcccatcag gatcgggatc actacgatcg ccagcagcgc cgcacccgcc atcgcatacg
    77761 ttttggtgaa cgccaacggt ccaaacagac gcccttcctg cccttccagg gtgaagatcg
    77821 ggataaacga caacgtgata atcagcagac tgataaatag cgccggcccc acttcaacag
    77881 acgcatcggt gatcacctgc cagcgcgttt tattatccag cgtggcgtca ggatgctggt
    77941 gctgccactc ttccagccgt ttatgtgcat tttcgatcat gacgatagcg gcatcgacca
    78001 tcgccccgac ggcaatcgca atgccaccca acgacataat attggcattc agtccctgga
    78061 agtgcatgac aataaaagca atacacaacc ccagcggcaa cgaaataatc gccaccagcg
    78121 ccgagcgcac atgccagaga aacagcgcac agactaccgc caccacaata aactcttcca
    78181 gcaacttgcc gctgaggttg tcgatggcgc ggtcaatgag ctggctgcga tcgtatgttg
    78241 taactatctc cacgccttcc ggcagactac ttttcagcgt ttccagtttg tccttcacgg
    78301 cggcgatcac ttctcgggcg tttttgccgg atcgcaggat caccaccccg cccgccactt
    78361 cgccttcgcc gtttagttcg gcaatgcccc ggcgcatctc cgggccaatc tggaccttcg
    78421 caacatcgcg cagataaacg ggcacgccat tttcactggc ttttaaaacg atgtgattaa
    78481 agtcgtcgag cgtttgcaga tagccgctgg cgcgcaccat atattccgct tccgccagtt
    78541 cgatcgacga accgcccgct tcctggtttg aagcatccag cgcgcttttt acttcggcga
    78601 gactgatgcc atactgcgcc aggcgctggg gatcgataac cacctgatac tctttcacca
    78661 caccgcccac cgacgccact tccgcaacgt cagggatggt tttcagctca tatttgagaa
    78721 accagtcctg taatgagcgt aaatcggcca gatcgtgctt accgctgcga tccaccagtg
    78781 catattcata gatccagcca acacccgtgg catctggtcc cagctcggcg ctgactcccg
    78841 caggcagctt accctgtacc tggttgaggt actccagcac ccgcgaacgc gcccagtacg
    78901 gatcggtgcc atcttcgaaa atgacataca cataagagtc gccaaactgt gagaaaccgc
    78961 gcacagtctt cgcgccaggc accgacaaca tggtggtggt tagcggataa gtcacctgat
    79021 tttcaacgat ttgtggtgcc tgaccgggat agctggtttt aataataacc tgcacatcgg
    79081 agagatccgg cagcgcatcc actggcgtat taatgatggt ccaggtgccc cagatgctca
    79141 gaaacaacgc gcccatcagc accaaaaaac ggttcgccac cgagcgacga ataatccatt
    79201 caatcattgg ttattccctc aatgcgcatg ggtagcactt tcagagcgca tccgctccag
    79261 tgcgccagaa atattggctt cggaatcaat caggaacagg ccgctggaaa ccaccttttc
    79321 accttccgcc agaccagagc gtaatgcggt gacgccttgc gatgcctgga aaacagcaac
    79381 gcgtttcggt acaaagcgcc cgtcggcatc aacggtaatc acccgctgtt cgctgccggt
    79441 atcaatcagc gcttgtgacg gaatgagcag catcggttcg ctggcggtgt tgagttgcaa
    79501 ccaggcgttc atgcccggtt ttagcgcctc gtcggcgttg tcgacttcca gacgcagctg
    79561 cagcgtgcgg gtcgcggcat ccacgccagg tagcagcgtc catttgcgga tggtgagtgt
    79621 tttatccggt cgcgccggaa cggtgagggt aaactgcgag gcatctttca ccagccaggc
    79681 gatagactcc gggatcgcag cagtgaccca caccgggtcc ataccctgaa ttttcgctac
    79741 cacgttatct ttggagatat tcattcccgc gcgcagatca aacgcggtga tcacgccatc
    79801 aatgggcgct ttgagcgtaa agcgagtctg gattttttgc gtggcgatca ggcggcgaat
    79861 atccgcctcc ggcattcccg ccagtcgcag ccgctcaaga atgccttcag tctgggtcgc
    79921 cgtaccgccg gtttcgcgca gcagtaaata ctcactctgc gcttccaccc agtcaggaat
    79981 ggtcaggtcg agaagcggtg tgcccttttg tactttatcg cccacggtaa gcggatacac
    80041 cttgtcgata aacccggcag cgcgagcctg cacaatggca tactgatact cgttgtaact
    80101 gacattcgcc gggaaactct gggcaaaagt cagcggcccg cgcgtgacgg tagccgtttt
    80161 cacccccaga ttctgcgtct gagtcgggtc aatgcgcaca ccagacgcag aactctcttc
    80221 atcggcatat ttcggcacca gatccatatc cataaacggc gatttacctg gtttatcgaa
    80281 ccgcgtattg gggtacatcg ggtcgtacca gaataagatt ttacgttctg cggtcgacgt
    80341 tttttctgcg ggtggttcag cctttgcaaa ccaggtaaaa cctgccgcag aaataatacc
    80401 gcccgcgatc atgctgccga taataagcgc gatttttttc atctcattaa acctgggtta
    80461 ctggctgact ttaatatcct gtaataaaga aaggttgccc tgctggacaa aattaaacgc
    80521 cactttgtcg ccggttttaa tttcactcat tttcgtctgc ggggtgatgg taaagcgcat
    80581 ggtcatctcc ggccagttca cagcagcaat cggatcgtga tggatggtga tttttttgct
    80641 ttccagatca atacccttta cctcgccagt ggcactaata acctgtggtt gtgcttcgct
    80701 catggtttca tgatgatgtt cgttagcctg ggcattaaag ccaataacgg taaacagact
    80761 gaacattgcg acttgcagtg cttttttcat ttattctctc ctggagttaa aagttattgc
    80821 tgctaaccgc caccaagtgc ggtatataaa gaaatttcgt taacctgacg ggcataattc
    80881 aggtcaagta aagtttgtcg ggttgcaaat aacgaacgtt cggcatccag cacttccaga
    80941 taacttactg cgccgtgctg atataatgcc cgcgcccgtt gcaaagtaat ttgcagcgat
    81001 gccagataac gctgctgggc gctaatttga tcgttcaggc tttgacgtaa tgccagcgca
    81061 tctgccactt ctttaaaggc gttctggatt ttctgttcat aattcactac cgactgctgc
    81121 tggcgaattt cggcgatatc cagattggcc tggttgcgtc c
//
LOCUS       DAZGAN020000008        76680 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGAN020000008 DAZGAN020000000
VERSION     DAZGAN020000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 76680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.008"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(323..1723)
                     /gene="asnS"
                     /locus_tag="ACHKYO_RS03115"
                     /old_locus_tag="pgaptmp_000623"
     CDS             complement(323..1723)
                     /gene="asnS"
                     /locus_tag="ACHKYO_RS03115"
                     /old_locus_tag="pgaptmp_000623"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGU4427518.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1892..3094)
                     /gene="pncB"
                     /locus_tag="ACHKYO_RS03120"
                     /old_locus_tag="pgaptmp_000624"
     CDS             complement(1892..3094)
                     /gene="pncB"
                     /locus_tag="ACHKYO_RS03120"
                     /old_locus_tag="pgaptmp_000624"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGU4427519.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3360..5972
                     /gene="pepN"
                     /locus_tag="ACHKYO_RS03125"
                     /old_locus_tag="pgaptmp_000625"
     CDS             3360..5972
                     /gene="pepN"
                     /locus_tag="ACHKYO_RS03125"
                     /old_locus_tag="pgaptmp_000625"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HGU4427520.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDELWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(6179..6946)
                     /gene="ssuB"
                     /locus_tag="ACHKYO_RS03130"
                     /old_locus_tag="pgaptmp_000626"
     CDS             complement(6179..6946)
                     /gene="ssuB"
                     /locus_tag="ACHKYO_RS03130"
                     /old_locus_tag="pgaptmp_000626"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU4427521.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(6943..7734)
                     /gene="ssuC"
                     /locus_tag="ACHKYO_RS03135"
                     /old_locus_tag="pgaptmp_000627"
     CDS             complement(6943..7734)
                     /gene="ssuC"
                     /locus_tag="ACHKYO_RS03135"
                     /old_locus_tag="pgaptmp_000627"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HGU4427522.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7745..8890)
                     /gene="ssuD"
                     /locus_tag="ACHKYO_RS03140"
                     /old_locus_tag="pgaptmp_000628"
     CDS             complement(7745..8890)
                     /gene="ssuD"
                     /locus_tag="ACHKYO_RS03140"
                     /old_locus_tag="pgaptmp_000628"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HGU4427523.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(8887..9846)
                     /gene="ssuA"
                     /locus_tag="ACHKYO_RS03145"
                     /old_locus_tag="pgaptmp_000629"
     CDS             complement(8887..9846)
                     /gene="ssuA"
                     /locus_tag="ACHKYO_RS03145"
                     /old_locus_tag="pgaptmp_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HGU4427524.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9839..10414)
                     /gene="ssuE"
                     /locus_tag="ACHKYO_RS03150"
                     /old_locus_tag="pgaptmp_000630"
     CDS             complement(9839..10414)
                     /gene="ssuE"
                     /locus_tag="ACHKYO_RS03150"
                     /old_locus_tag="pgaptmp_000630"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGU4427525.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADRLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            10770..11312
                     /locus_tag="ACHKYO_RS03155"
                     /old_locus_tag="pgaptmp_000631"
     CDS             10770..11312
                     /locus_tag="ACHKYO_RS03155"
                     /old_locus_tag="pgaptmp_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4427526.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            11395..12096
                     /gene="elfD"
                     /locus_tag="ACHKYO_RS03160"
                     /old_locus_tag="pgaptmp_000632"
     CDS             11395..12096
                     /gene="elfD"
                     /locus_tag="ACHKYO_RS03160"
                     /old_locus_tag="pgaptmp_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4427527.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            12121..14721
                     /gene="elfC"
                     /locus_tag="ACHKYO_RS03165"
                     /old_locus_tag="pgaptmp_000633"
     CDS             12121..14721
                     /gene="elfC"
                     /locus_tag="ACHKYO_RS03165"
                     /old_locus_tag="pgaptmp_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGU4427528.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     IWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDVTQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSLGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            14712..15782
                     /gene="elfG"
                     /locus_tag="ACHKYO_RS03170"
                     /old_locus_tag="pgaptmp_000634"
     CDS             14712..15782
                     /gene="elfG"
                     /locus_tag="ACHKYO_RS03170"
                     /old_locus_tag="pgaptmp_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4427529.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            15794..16336
                     /gene="ycbU"
                     /locus_tag="ACHKYO_RS03175"
                     /old_locus_tag="pgaptmp_000635"
     CDS             15794..16336
                     /gene="ycbU"
                     /locus_tag="ACHKYO_RS03175"
                     /old_locus_tag="pgaptmp_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4427530.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            16344..16859
                     /gene="ycbV"
                     /locus_tag="ACHKYO_RS03180"
                     /old_locus_tag="pgaptmp_000636"
     CDS             16344..16859
                     /gene="ycbV"
                     /locus_tag="ACHKYO_RS03180"
                     /old_locus_tag="pgaptmp_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4427531.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            16825..17562
                     /gene="ycbF"
                     /locus_tag="ACHKYO_RS03185"
                     /old_locus_tag="pgaptmp_000637"
     CDS             16825..17562
                     /gene="ycbF"
                     /locus_tag="ACHKYO_RS03185"
                     /old_locus_tag="pgaptmp_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU4427532.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAIRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTWNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            17673..18683
                     /gene="pyrD"
                     /locus_tag="ACHKYO_RS03190"
                     /old_locus_tag="pgaptmp_000638"
     CDS             17673..18683
                     /gene="pyrD"
                     /locus_tag="ACHKYO_RS03190"
                     /old_locus_tag="pgaptmp_000638"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HGU4427533.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            18857..19399
                     /gene="zapC"
                     /locus_tag="ACHKYO_RS03195"
                     /old_locus_tag="pgaptmp_000639"
     CDS             18857..19399
                     /gene="zapC"
                     /locus_tag="ACHKYO_RS03195"
                     /old_locus_tag="pgaptmp_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HGU4427534.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMRKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(19396..20505)
                     /gene="ycbX"
                     /locus_tag="ACHKYO_RS03200"
                     /old_locus_tag="pgaptmp_000640"
     CDS             complement(19396..20505)
                     /gene="ycbX"
                     /locus_tag="ACHKYO_RS03200"
                     /old_locus_tag="pgaptmp_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HGU4427535.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            20749..22857
                     /gene="rlmKL"
                     /locus_tag="ACHKYO_RS03205"
                     /old_locus_tag="pgaptmp_000641"
     CDS             20749..22857
                     /gene="rlmKL"
                     /locus_tag="ACHKYO_RS03205"
                     /old_locus_tag="pgaptmp_000641"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HGU4427536.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEDIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            22869..24776
                     /gene="uup"
                     /locus_tag="ACHKYO_RS03210"
                     /old_locus_tag="pgaptmp_000642"
     CDS             22869..24776
                     /gene="uup"
                     /locus_tag="ACHKYO_RS03210"
                     /old_locus_tag="pgaptmp_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4427537.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            24906..26159
                     /gene="pqiA"
                     /locus_tag="ACHKYO_RS03215"
                     /old_locus_tag="pgaptmp_000643"
     CDS             24906..26159
                     /gene="pqiA"
                     /locus_tag="ACHKYO_RS03215"
                     /old_locus_tag="pgaptmp_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HGU4427538.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            26164..27804
                     /gene="pqiB"
                     /locus_tag="ACHKYO_RS03220"
                     /old_locus_tag="pgaptmp_000644"
     CDS             26164..27804
                     /gene="pqiB"
                     /locus_tag="ACHKYO_RS03220"
                     /old_locus_tag="pgaptmp_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HGU4427539.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            27801..28364
                     /gene="pqiC"
                     /locus_tag="ACHKYO_RS03225"
                     /old_locus_tag="pgaptmp_000645"
     CDS             27801..28364
                     /gene="pqiC"
                     /locus_tag="ACHKYO_RS03225"
                     /old_locus_tag="pgaptmp_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HGU4427540.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            28620..28787
                     /gene="rmf"
                     /locus_tag="ACHKYO_RS03230"
                     /old_locus_tag="pgaptmp_000646"
     CDS             28620..28787
                     /gene="rmf"
                     /locus_tag="ACHKYO_RS03230"
                     /old_locus_tag="pgaptmp_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HGU4427541.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(28857..29375)
                     /gene="fabA"
                     /locus_tag="ACHKYO_RS03235"
                     /old_locus_tag="pgaptmp_000647"
     CDS             complement(28857..29375)
                     /gene="fabA"
                     /locus_tag="ACHKYO_RS03235"
                     /old_locus_tag="pgaptmp_000647"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HGU4427542.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(29444..31204)
                     /gene="ycbZ"
                     /locus_tag="ACHKYO_RS03240"
                     /old_locus_tag="pgaptmp_000648"
     CDS             complement(29444..31204)
                     /gene="ycbZ"
                     /locus_tag="ACHKYO_RS03240"
                     /old_locus_tag="pgaptmp_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4427543.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            31390..31842
                     /gene="matP"
                     /locus_tag="ACHKYO_RS03245"
                     /old_locus_tag="pgaptmp_000649"
     CDS             31390..31842
                     /gene="matP"
                     /locus_tag="ACHKYO_RS03245"
                     /old_locus_tag="pgaptmp_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HGU4427544.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(31918..32958)
                     /gene="ompA"
                     /locus_tag="ACHKYO_RS03250"
                     /old_locus_tag="pgaptmp_000650"
     CDS             complement(31918..32958)
                     /gene="ompA"
                     /locus_tag="ACHKYO_RS03250"
                     /old_locus_tag="pgaptmp_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HGU4427545.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(33315..33824)
                     /gene="sulA"
                     /locus_tag="ACHKYO_RS03255"
                     /old_locus_tag="pgaptmp_000651"
     CDS             complement(33315..33824)
                     /gene="sulA"
                     /locus_tag="ACHKYO_RS03255"
                     /old_locus_tag="pgaptmp_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HGU4427546.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            34043..34672
                     /gene="sxy"
                     /locus_tag="ACHKYO_RS03260"
                     /old_locus_tag="pgaptmp_000652"
     CDS             34043..34672
                     /gene="sxy"
                     /locus_tag="ACHKYO_RS03260"
                     /old_locus_tag="pgaptmp_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HGU4427547.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(34635..36797)
                     /gene="yccS"
                     /locus_tag="ACHKYO_RS03265"
                     /old_locus_tag="pgaptmp_000653"
     CDS             complement(34635..36797)
                     /gene="yccS"
                     /locus_tag="ACHKYO_RS03265"
                     /old_locus_tag="pgaptmp_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HGU4427548.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAIRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDEAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(36807..37253)
                     /gene="yccF"
                     /locus_tag="ACHKYO_RS03270"
                     /old_locus_tag="pgaptmp_000654"
     CDS             complement(36807..37253)
                     /gene="yccF"
                     /locus_tag="ACHKYO_RS03270"
                     /old_locus_tag="pgaptmp_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HGU4427549.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            37376..39430
                     /gene="helD"
                     /locus_tag="ACHKYO_RS03275"
                     /old_locus_tag="pgaptmp_000655"
     CDS             37376..39430
                     /gene="helD"
                     /locus_tag="ACHKYO_RS03275"
                     /old_locus_tag="pgaptmp_000655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HGU4427550.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(39462..39920)
                     /gene="mgsA"
                     /locus_tag="ACHKYO_RS03280"
                     /old_locus_tag="pgaptmp_000656"
     CDS             complement(39462..39920)
                     /gene="mgsA"
                     /locus_tag="ACHKYO_RS03280"
                     /old_locus_tag="pgaptmp_000656"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HGU4427551.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(40016..40678)
                     /gene="yccT"
                     /locus_tag="ACHKYO_RS03285"
                     /old_locus_tag="pgaptmp_000657"
     CDS             complement(40016..40678)
                     /gene="yccT"
                     /locus_tag="ACHKYO_RS03285"
                     /old_locus_tag="pgaptmp_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HGU4427552.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            40851..41264
                     /gene="yccU"
                     /locus_tag="ACHKYO_RS03290"
                     /old_locus_tag="pgaptmp_000658"
     CDS             40851..41264
                     /gene="yccU"
                     /locus_tag="ACHKYO_RS03290"
                     /old_locus_tag="pgaptmp_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGU4427553.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(41309..41626)
                     /gene="hspQ"
                     /locus_tag="ACHKYO_RS03295"
                     /old_locus_tag="pgaptmp_000659"
     CDS             complement(41309..41626)
                     /gene="hspQ"
                     /locus_tag="ACHKYO_RS03295"
                     /old_locus_tag="pgaptmp_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HGU4427554.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(41684..42874)
                     /gene="rlmI"
                     /locus_tag="ACHKYO_RS03300"
                     /old_locus_tag="pgaptmp_000660"
     CDS             complement(41684..42874)
                     /gene="rlmI"
                     /locus_tag="ACHKYO_RS03300"
                     /old_locus_tag="pgaptmp_000660"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HGU4427555.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECSIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            42969..43247
                     /gene="yccX"
                     /locus_tag="ACHKYO_RS03305"
                     /old_locus_tag="pgaptmp_000661"
     CDS             42969..43247
                     /gene="yccX"
                     /locus_tag="ACHKYO_RS03305"
                     /old_locus_tag="pgaptmp_000661"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGU4427556.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(43244..43573)
                     /gene="tusE"
                     /locus_tag="ACHKYO_RS03310"
                     /old_locus_tag="pgaptmp_000662"
     CDS             complement(43244..43573)
                     /gene="tusE"
                     /locus_tag="ACHKYO_RS03310"
                     /old_locus_tag="pgaptmp_000662"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HGU4427557.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(43664..44323)
                     /gene="yccA"
                     /locus_tag="ACHKYO_RS03315"
                     /old_locus_tag="pgaptmp_000663"
     CDS             complement(43664..44323)
                     /gene="yccA"
                     /locus_tag="ACHKYO_RS03315"
                     /old_locus_tag="pgaptmp_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HGU4427558.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(44530..44617)
                     /locus_tag="ACHKYO_RS03320"
                     /old_locus_tag="pgaptmp_000664"
     tRNA            complement(44530..44617)
                     /locus_tag="ACHKYO_RS03320"
                     /old_locus_tag="pgaptmp_000664"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44581..44583),aa:Ser,seq:tga)
     gene            45044..46162
                     /gene="hyaA"
                     /locus_tag="ACHKYO_RS03325"
                     /old_locus_tag="pgaptmp_000665"
     CDS             45044..46162
                     /gene="hyaA"
                     /locus_tag="ACHKYO_RS03325"
                     /old_locus_tag="pgaptmp_000665"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HGU4427559.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            46159..47952
                     /gene="hyaB"
                     /locus_tag="ACHKYO_RS03330"
                     /old_locus_tag="pgaptmp_000666"
     CDS             46159..47952
                     /gene="hyaB"
                     /locus_tag="ACHKYO_RS03330"
                     /old_locus_tag="pgaptmp_000666"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HGU4427560.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            47971..48678
                     /gene="hyaC"
                     /locus_tag="ACHKYO_RS03335"
                     /old_locus_tag="pgaptmp_000667"
     CDS             47971..48678
                     /gene="hyaC"
                     /locus_tag="ACHKYO_RS03335"
                     /old_locus_tag="pgaptmp_000667"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HGU4427561.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            48675..49262
                     /gene="hyaD"
                     /locus_tag="ACHKYO_RS03340"
                     /old_locus_tag="pgaptmp_000668"
     CDS             48675..49262
                     /gene="hyaD"
                     /locus_tag="ACHKYO_RS03340"
                     /old_locus_tag="pgaptmp_000668"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HGU4427562.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELARDQLPAAEQSALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            49259..49657
                     /gene="hyaE"
                     /locus_tag="ACHKYO_RS03345"
                     /old_locus_tag="pgaptmp_000669"
     CDS             49259..49657
                     /gene="hyaE"
                     /locus_tag="ACHKYO_RS03345"
                     /old_locus_tag="pgaptmp_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HGU4427563.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            49654..50511
                     /gene="hyaF"
                     /locus_tag="ACHKYO_RS03350"
                     /old_locus_tag="pgaptmp_000670"
     CDS             49654..50511
                     /gene="hyaF"
                     /locus_tag="ACHKYO_RS03350"
                     /old_locus_tag="pgaptmp_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HGU4427564.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQMSQRQPPLGEVLAVDLLNLHADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            50645..52189
                     /gene="appC"
                     /locus_tag="ACHKYO_RS03355"
                     /old_locus_tag="pgaptmp_000671"
     CDS             50645..52189
                     /gene="appC"
                     /locus_tag="ACHKYO_RS03355"
                     /old_locus_tag="pgaptmp_000671"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HGU4427565.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            52201..53337
                     /gene="appB"
                     /locus_tag="ACHKYO_RS03360"
                     /old_locus_tag="pgaptmp_000672"
     CDS             52201..53337
                     /gene="appB"
                     /locus_tag="ACHKYO_RS03360"
                     /old_locus_tag="pgaptmp_000672"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU4427566.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            53350..53442
                     /gene="cbdX"
                     /locus_tag="ACHKYO_RS03365"
                     /old_locus_tag="pgaptmp_000673"
     CDS             53350..53442
                     /gene="cbdX"
                     /locus_tag="ACHKYO_RS03365"
                     /old_locus_tag="pgaptmp_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HGU4427567.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            53522..54820
                     /gene="appA"
                     /locus_tag="ACHKYO_RS03370"
                     /old_locus_tag="pgaptmp_000674"
     CDS             53522..54820
                     /gene="appA"
                     /locus_tag="ACHKYO_RS03370"
                     /old_locus_tag="pgaptmp_000674"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HGU4427568.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSRQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(54935..57115)
                     /gene="etk"
                     /locus_tag="ACHKYO_RS03375"
                     /old_locus_tag="pgaptmp_000675"
     CDS             complement(54935..57115)
                     /gene="etk"
                     /locus_tag="ACHKYO_RS03375"
                     /old_locus_tag="pgaptmp_000675"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HGU4427569.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(57135..57581)
                     /gene="etp"
                     /locus_tag="ACHKYO_RS03380"
                     /old_locus_tag="pgaptmp_000676"
                     /gene_synonym="yccY"
     CDS             complement(57135..57581)
                     /gene="etp"
                     /locus_tag="ACHKYO_RS03380"
                     /old_locus_tag="pgaptmp_000676"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HGU4427570.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(57569..58708)
                     /gene="gfcE"
                     /locus_tag="ACHKYO_RS03385"
                     /old_locus_tag="pgaptmp_000677"
     CDS             complement(57569..58708)
                     /gene="gfcE"
                     /locus_tag="ACHKYO_RS03385"
                     /old_locus_tag="pgaptmp_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HGU4427571.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(58754..60850)
                     /gene="gfcD"
                     /locus_tag="ACHKYO_RS03390"
                     /old_locus_tag="pgaptmp_000678"
     CDS             complement(58754..60850)
                     /gene="gfcD"
                     /locus_tag="ACHKYO_RS03390"
                     /old_locus_tag="pgaptmp_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU4427572.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(60850..61596)
                     /gene="gfcC"
                     /locus_tag="ACHKYO_RS03395"
                     /old_locus_tag="pgaptmp_000679"
     CDS             complement(60850..61596)
                     /gene="gfcC"
                     /locus_tag="ACHKYO_RS03395"
                     /old_locus_tag="pgaptmp_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU4427573.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(61593..62237)
                     /gene="gfcB"
                     /locus_tag="ACHKYO_RS03400"
                     /old_locus_tag="pgaptmp_000680"
     CDS             complement(61593..62237)
                     /gene="gfcB"
                     /locus_tag="ACHKYO_RS03400"
                     /old_locus_tag="pgaptmp_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HGU4427574.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(62344..62649)
                     /gene="gfcA"
                     /locus_tag="ACHKYO_RS03405"
                     /old_locus_tag="pgaptmp_000681"
     CDS             complement(62344..62649)
                     /gene="gfcA"
                     /locus_tag="ACHKYO_RS03405"
                     /old_locus_tag="pgaptmp_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HGU4427575.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(63091..63303)
                     /gene="cspH"
                     /locus_tag="ACHKYO_RS03410"
                     /old_locus_tag="pgaptmp_000682"
     CDS             complement(63091..63303)
                     /gene="cspH"
                     /locus_tag="ACHKYO_RS03410"
                     /old_locus_tag="pgaptmp_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HGU4427576.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            63589..63801
                     /gene="cspG"
                     /locus_tag="ACHKYO_RS03415"
                     /old_locus_tag="pgaptmp_000683"
     CDS             63589..63801
                     /gene="cspG"
                     /locus_tag="ACHKYO_RS03415"
                     /old_locus_tag="pgaptmp_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HGU4427577.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            63812..64000
                     /gene="ymcF"
                     /locus_tag="ACHKYO_RS03420"
                     /old_locus_tag="pgaptmp_000684"
     CDS             63812..64000
                     /gene="ymcF"
                     /locus_tag="ACHKYO_RS03420"
                     /old_locus_tag="pgaptmp_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU4427578.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            63975..64205
                     /gene="ymcE"
                     /locus_tag="ACHKYO_RS03425"
                     /old_locus_tag="pgaptmp_000685"
     CDS             63975..64205
                     /gene="ymcE"
                     /locus_tag="ACHKYO_RS03425"
                     /old_locus_tag="pgaptmp_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HGU4427579.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            64195..64368
                     /gene="gnsA"
                     /locus_tag="ACHKYO_RS03430"
                     /old_locus_tag="pgaptmp_000686"
     CDS             64195..64368
                     /gene="gnsA"
                     /locus_tag="ACHKYO_RS03430"
                     /old_locus_tag="pgaptmp_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGU4427580.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(64416..65489)
                     /gene="yccM"
                     /locus_tag="ACHKYO_RS03435"
                     /old_locus_tag="pgaptmp_000687"
     CDS             complement(64416..65489)
                     /gene="yccM"
                     /locus_tag="ACHKYO_RS03435"
                     /old_locus_tag="pgaptmp_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU4427581.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(65561..68305)
                     /gene="torS"
                     /locus_tag="ACHKYO_RS03440"
                     /old_locus_tag="pgaptmp_000688"
     CDS             complement(65561..68305)
                     /gene="torS"
                     /locus_tag="ACHKYO_RS03440"
                     /old_locus_tag="pgaptmp_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HGU4427582.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYF
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSNLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            68388..69416
                     /gene="torT"
                     /locus_tag="ACHKYO_RS03445"
                     /old_locus_tag="pgaptmp_000689"
     CDS             68388..69416
                     /gene="torT"
                     /locus_tag="ACHKYO_RS03445"
                     /old_locus_tag="pgaptmp_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HGU4427583.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(69389..70081)
                     /gene="torR"
                     /locus_tag="ACHKYO_RS03450"
                     /old_locus_tag="pgaptmp_000690"
     CDS             complement(69389..70081)
                     /gene="torR"
                     /locus_tag="ACHKYO_RS03450"
                     /old_locus_tag="pgaptmp_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HGU4427584.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            70211..71383
                     /gene="torC"
                     /locus_tag="ACHKYO_RS03455"
                     /old_locus_tag="pgaptmp_000691"
     CDS             70211..71383
                     /gene="torC"
                     /locus_tag="ACHKYO_RS03455"
                     /old_locus_tag="pgaptmp_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HGU4427585.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            71383..73929
                     /gene="torA"
                     /locus_tag="ACHKYO_RS03460"
                     /old_locus_tag="pgaptmp_000692"
     CDS             71383..73929
                     /gene="torA"
                     /locus_tag="ACHKYO_RS03460"
                     /old_locus_tag="pgaptmp_000692"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HGU4427586.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAESIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            73926..74525
                     /gene="torD"
                     /locus_tag="ACHKYO_RS03465"
                     /old_locus_tag="pgaptmp_000693"
     CDS             73926..74525
                     /gene="torD"
                     /locus_tag="ACHKYO_RS03465"
                     /old_locus_tag="pgaptmp_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HGU4427587.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(74618..74923)
                     /gene="cbpM"
                     /locus_tag="ACHKYO_RS03470"
                     /old_locus_tag="pgaptmp_000694"
     CDS             complement(74618..74923)
                     /gene="cbpM"
                     /locus_tag="ACHKYO_RS03470"
                     /old_locus_tag="pgaptmp_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HGU4427588.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(74923..75843)
                     /gene="cbpA"
                     /locus_tag="ACHKYO_RS03475"
                     /old_locus_tag="pgaptmp_000695"
     CDS             complement(74923..75843)
                     /gene="cbpA"
                     /locus_tag="ACHKYO_RS03475"
                     /old_locus_tag="pgaptmp_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HGU4427589.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            76104..>76680
                     /locus_tag="ACHKYO_RS03480"
                     /old_locus_tag="pgaptmp_000696"
     CDS             76104..>76680
                     /locus_tag="ACHKYO_RS03480"
                     /old_locus_tag="pgaptmp_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4427590.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQNVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENITACDDVFSEYCFD
                     DENMSIQPEQINTPGISDLDTGIDLSGISFIQ"
BASE COUNT        19039 a        19056 c        20014 g        18571 t
ORIGIN      
        1 tatttatatg atttacttat atttaaatta actaaacgga aattttgttt ctgatggaaa
       61 ctttatcgac ctggcacaaa atcctctttc agacatccag aatgccaaaa aatagtatga
      121 aaattgtgct atcagctaag aaaaaactaa tccgcaataa gaatgcgggt tattttttgt
      181 gcgtaaatgt tgcaatgtaa tattccgtaa caggatgatc gttatggcgg ggttgcaaat
      241 agattgcttg acttaacaaa cagataactt gacagaaagg ataaaaaaga gccagcaggc
      301 gctggctctt tgaggaagaa aatcagaagc tggcgttgcg cggagtacgt gggaacggaa
      361 tcacatcacg cacgttttgc acgccagtta cgtaagcaat caggcgttca aaaccaagac
      421 cgaaacctga atgcggaaca gtaccgtagc gacgcagatc gcgataccac cagtaatctt
      481 ctttattcag gcccatttcc agcatacgct cgtccagaac gtccagacgt tcttcacgct
      541 gggagccacc aatgatctca ccgatgcccg gagccagaac gtccatagcc gcaacggttt
      601 taccgtcttc gttgaggcgc atatagaacg ctttaatatc tttcggatag tttttaacca
      661 ctaccggtgc tttaaagtgt tcttccgcca gataacgctc atgctcagaa gagagatcga
      721 cgccccagta aaccgggttt tcaaacttcc tgccgcagtt ttcgagaatg gtcactgcgt
      781 cggtgtaatc cacctgcgcg aaatcggcct caatgaagcg ttccagacgt gaaacggcat
      841 ctttatctac gcgttcagcg aagaatttca tgtcgtctgc gcgttcttcg agaaccgctt
      901 tgaagacata tttcagcatg gcttcagcca gacccgcgat atcgttcagg ttagcaaacg
      961 ccacttccgg ctccagcatc cagaattccg ccaggtgacg gctggtgttg gagttttcag
     1021 cacggaaagt cgggccgaag gtataaattt tggataatgc gcaagcgtag gtttcgccgt
     1081 tcaactggcc agatacggtc aggaaagact ctttaccaaa gaagtctttg tcgaaatcca
     1141 ctttgccctg atcgttacgc ggcaggtttt ccagatccag cgtagaaacg cggaacattt
     1201 cgcctgcacc ttcggtatca gatgcggtaa tcagtggcgt tgaaacccag aagaatccct
     1261 gctcgttaaa gaagcgatgc agcgcctgcg ccagcgtatg gcgaacgcgc gcgacggcac
     1321 caatcaggtt tgtgcgcgga cgcaggtgag cgacttcacg cagatactca atgctgtggc
     1381 gttttgccgc catcgggtaa gtgtctggat cttcaaccca accagcaact tcaactttgc
     1441 tggcctgaat ttcaaattgt tgcccctggc ccggcgacgc cacgacttta cccgtcacaa
     1501 tgaccgagca gccggtggtc agacgcagga cgtcttcatt gtaattgggc agagaattat
     1561 tgatgacagc ctgtacagga tcaaagcagg aaccgtcata aacggcgagg aaggagatgc
     1621 cagcttttga atctcggcgg gtacgtaccc atccgcgtac ggtgacttcg ctgtcaacgg
     1681 ctacgcggcc ctggagtacg tcggctacag gcacaacgct cataatattc tctctgttaa
     1741 tagtcggaaa aaataaacac ttgtccaccc gtaaatgggg ggatacctat gttacctggc
     1801 atctgcaatc agacaagcag aattcgcaaa tgcagcgaaa gatttcggaa ttaaaggtaa
     1861 aaaaagggag ccgattagct ccctgatgat attaactggc ttttttaata tgcggaaggt
     1921 cgaacgcttt gcgcagcgcc cgaacaaacg ctttatcgtg gcagatagtt ttgccagggc
     1981 tgtcagaaag tttcgccacc ggtttaccgt tacactcaac caacttaatc acaatattca
     2041 ggggttttac ctgggggata tcgcaggtca ggcgagtccc aataccaaaa cttaattgca
     2101 cgcgggaaga gaagtggcgg tatagctcaa ccgctttgcg taaatccaga ttgtcagaga
     2161 aaaccagagt tttactctgt ggatcaattc ccagcttttc ataatgtgca atggcttttt
     2221 caccccattc aaccgggtcg ccagagtcat gacgcaggcc ctgatagcga ctggcgaact
     2281 cgacaccgaa atcacgcagg aaagcatcca tagtgatgca gtcggttaat gcaatgccaa
     2341 gttggtcggg atattcttcc agccaggcag caagtgcagc tcgctggctg ttggctagat
     2401 ccgggctgat ttgctgatgt gcctggaacc attcgtgtgc ctgtgttccc atcggcgtga
     2461 gggaaagccg acgcgccaga tcgtagttgc tggtgcccac gaaccaggat tcctgttgca
     2521 gacgcttaac gatggtttct tgtacttcgc gagaaaaacg gcgacgggtg ccaaaatcca
     2581 taagatggaa gcgcgacata tcaaggccgg cggttaacgc cgagaagtcg actaatttgc
     2641 tttccagcgt gtcgagggct tgcgcaacgt cggcctgcgg tgagcgatag cgatgtacca
     2701 tttcactgat aaccgccagc aaaggaactt cccagaggat gacttcacgc cacgggccgc
     2761 ttaaacgaat atccagcttg ccattatcgt tggatacggt gacttgttcc gggttaaagc
     2821 ggaactcgcg taaccagtta agatagtcgg ccttaaagaa aggcagagca gagagccact
     2881 gatattcatc atcctgcagg cgcaggtgct gcatcgcctg aatctgttca cgaatagcat
     2941 cggcataaat acccagcaga tcgtcacctc ggcaacgaaa ctccgccgcg acatgcacat
     3001 cgtaatagtg atgaaacacg gcttgctgca tatgcaactt ataagcatct gtatccagca
     3061 acgagtgcag aacaggagaa gcgaattgtg tcataggtgc gcagtagcgt cctcgtacag
     3121 gagcgtttag tacaataaac atcttcagga actgctggag tataccttgt ttcgcaattt
     3181 attgaacccc gatcacacca tatgccacct ttctggtcga tggcattacg cggtgtatgt
     3241 tataaaaatg tagcaataaa ggcgtttgta cctgaaaaga tgaagattct gcatagcgcg
     3301 atttacgcaa caggaataga ctgaacacca gactctataa aagatgctaa aggttattta
     3361 tgactcaaca gccacaagcc aaataccgtc acgattatcg tgcgccggat taccagatta
     3421 ctgatattga cttgaccttt gacctcgacg cgcaaaagac ggtcgttacc gcggtcagcc
     3481 aggctgtccg tcatggtgca tcagatgctc cgcttcgtct caacggcgaa gacctcaaac
     3541 tggtttctgt tcatattaat gatgagctgt ggaccgcctg gaaagaagaa gagggcgcac
     3601 tggtcatcag taatttgccg gagcgtttta cgcttaagat cattaatgaa ataagcccgg
     3661 cggcgaatac ggcgctggaa gggctttatc agtcaggcga tgcgctttgc acccagtgtg
     3721 aagccgaagg tttccgccat attacgtatt atctcgaccg cccggacgtg ctggcgcgtt
     3781 ttaccaccaa aattattgcc gataaaatca aatatccctt cctgctttcc aatggtaacc
     3841 gcgttgcgca aggcgaactc gaaaacggac gccattgggt acagtggcag gacccgttcc
     3901 cgaaaccgtg ctacctgttt gcgctggtgg caggcgactt tgatgtactg cgcgatacct
     3961 ttaccacgcg ttctggtcgc gaagtagcac tggagctgta cgtcgatcgc ggcaaccttg
     4021 atcgcgcgcc gtgggcgatg acctcgctga aaaactccat gaaatgggat gaagaacgct
     4081 ttggcctgga gtatgacctc gacatctata tgatcgtcgc ggtggatttc ttcaatatgg
     4141 gcgcaatgga gaataagggg ctgaatatct ttaactccaa atatgtgttg gcccgcaccg
     4201 acaccgccac cgacaaagat tatctcgata ttgaacgcgt tatcggccat gaatatttcc
     4261 ataactggac cggtaaccga gtgacctgcc gcgactggtt ccagctcagc ctgaaagaag
     4321 gtttaaccgt cttccgcgat caggaattca gctctgacct tggttcccgc gcggtaaacc
     4381 gtatcaataa tgtgcgcacc atgcgcggat tgcagtttgc ggaagacgcc agcccgatgg
     4441 cgcacccgat ccgcccggat atggtcattg agatgaacaa cttctacacc ctgaccgttt
     4501 acgagaaggg cgcggaagtg attcgcatga tccacaccct gctgggcgaa gaaaacttcc
     4561 agaaagggat gcaactctat ttcgagcgcc atgatggcag cgcggcaacc tgcgacgact
     4621 ttgtgcaggc gatggaagat gcgtcgaatg tcgatctctc ccatttccgc cgttggtaca
     4681 gccagtccgg cacgccgatt gtgaccgtca aagacgacta caatccggaa accgagcagt
     4741 acaccctgac catcagccag cgcacgccag ccacgccgga tcaggcagaa aaacagccgc
     4801 tgcatattcc atttgccatc gaattgtatg acaacgaagg caaagtgatc ccgttgcaga
     4861 aaggcggtca tccggtgaat tccgtgctga acgtcaccca ggcggaacag acctttgttt
     4921 ttgataatgt ctacttccag ccggtgcctg cgctgctgtg cgaattctct gcgccagtga
     4981 aactggaata taagtggagc gatcagcaac tgaccttcct gatgcgtcat gcgcgtaatg
     5041 atttctcccg ttgggatgcg gcgcaaagtt tgctggcaac ctacatcaag ctgaacgtcg
     5101 cccgtcatca gcaagggcag ccgctgtctc tgccggtaca tgtggctgac gctttccgcg
     5161 cggtgctgct cgatgagaag attgatccgg cgctggcggc agaaatcctg acgctgcctt
     5221 ctgtcaatga aatggctgaa ctgttcgata tcatcgaccc gattgctatt gccgaagtac
     5281 gcgaagcact cactcgtact ctggcgactg aactggcgga tgagctgctg gctatttaca
     5341 acgcgaatta ccagagcgag taccgtgttg agcatgaaga tattgcaaaa cgcactctgc
     5401 gtaatgcctg cctgcgcttc cttgcttttg gtgaaacgca tctggctgac gtgctggtga
     5461 gcaagcaata ccatgaagca aacaatatga ccgatgcgct ggcagcgctt tctgcggcgg
     5521 ttgctgcaca gctgccttgc cgtgacgcgc tgatgcagga gtacgacgat aagtggcatc
     5581 aggacggtct ggtgatggat aaatggttta tcctgcaagc caccagcccg gcggcgaatg
     5641 tgctggagac ggtgcgtggt ctgttgcagc atcgctcatt taccatgagc aacccgaacc
     5701 gtattcgttc gttgattggt gcgtttgcgg gcagcaaccc ggcagcgttc catgccgaag
     5761 atggcagcgg ttaccagttc ctggtggaaa tgcttaccga cctcaacagc cgtaacccgc
     5821 aggtagcttc acgtctgatt gaaccgctga ttcgcctgaa acgttatgat gccaaacgtc
     5881 aggagaaaat gcgcgcggcg ctggaacagt tgaaagggct ggaaaatctc tctggcgatc
     5941 tgtacgagaa gatcaccaaa gcactggctt gatacttttg agccaaaatg cctgatgcga
     6001 cgcttgacgc gtcttatcag gcctacaact ccgttgaaat tgctggtttt gtaggctgga
     6061 taaggcgttc acgccgcatc cggcagccgt gcccaactgc ctgatgcgat gcttgacgtg
     6121 tcttatcagg cctactagtc cgttgaaaat gctggttttg tgcgccacat ccggcatatt
     6181 aaccatgacg ccgaatcagt tgttcagaat gcccacgccg cattacccgt tgcaatactt
     6241 ccgcttccag ctccgctaat cgcaccgacc ctaatcgacg cggacggggg atatccactg
     6301 tcaaatccag accaattttt ccctcttcaa ttaacaacac ccggtcagcc attgctaccg
     6361 cttcgctcac atcatgcgtc accaacagta cggtaaagcc gtgctcctgc caaagtgaca
     6421 caatcaaatc ctgcatctcg agtcgcgtta aggcgtccag tgccccgagc ggttcatcaa
     6481 gcaacaataa acccggtcga tgaatcaacg ctcttgccag cgccactcgc tgtttctgcc
     6541 cgcctgaaag tgcggcaggc cattccccgg cgcgattctc cagtcctacc gcagccaacg
     6601 cccgacgcgc ggcatcgcgc cactggcctt taaggcctaa cccaacgtta tcaatcaccg
     6661 atttccacgg tagcagacgc gcatcctgaa acatcattcg cgtgtcttcc tgaatttcag
     6721 ccagcggtgt ggtgcctgcc aacacatcgc ctgcggttgg cgtttccagc ccggccagca
     6781 ggcgcagcag ggtacttttg ccaccaccgc tgcggcccac caccgccaca aactgacctg
     6841 ccggaatatg taaatccagt tggttcagga cgatattttc cgcgtaatgt ttgcttactg
     6901 cattgagcaa caatggtgtg ccctggttca gacgagcagt attcataccg tggcctcctt
     6961 caaatgataa gccgggttcc agcgcaacca gaggcgctct aacagctgcg cgctgacgtc
     7021 agcaagtttg ccgagcaggg cgtaaagaat aatagcgact accaccacgt ccgtttgcaa
     7081 aaactcccgc gcattcatcg ccagataacc aatgcctgaa ttggcagaaa tggtttcggc
     7141 aacaatcagc gtcagccaca tcaggcccaa cgcaaaacgc acgccgacca taattgaggg
     7201 cagggcacca ggcaggatca catggataaa cagcggtatg ccggataatc catagctacg
     7261 cgccatttcc accagaccgc gatcgatatt acggatgcca tgccaggtgt tgatataaat
     7321 ggggaatagc gtgccgagcg ccaccagaaa gatttttgcg gattcatcaa tgccaaacca
     7381 caaaatcacc agcgggatca gcgccagatg cggtacgttg cgcaacatct gaattgaggt
     7441 atccagcagc cgttctcccc agcgagatag cccgctaatc agtcccaaaa tcaaccccag
     7501 cgatccgcca attgaaaagc caatcagcgc ccgccaggaa ctgatcgcca gatgctgcca
     7561 cagttcgccg ctggcggaga gcgtccagaa cgccgtcacc accccttccg gtgagggcaa
     7621 aatacgcgtc gacagccagc caactgagga ggccagttgc cacaccgcca cgatgcccac
     7681 cggtaaaaac cagggggcaa cgcgcaataa ccacttcttc actggcgttg ccattatttc
     7741 ttccttagct ttgcgcgact ttacggggga taaaatcatt cgccaccgct tcgccttgcg
     7801 gattcagcgg ctgcggctgg ggaatttccg gaatggcgac atccagatgt gggaacagca
     7861 actcgccgac ccgatacgct tcttccagat gcggatagcc cgaaagcaca aagctgtcga
     7921 tgccaagcgc ggcgtattcg ttgattcgcg cagcgaccgt aggaccatcg cccaccagcg
     7981 ccgttccggc accgccgcgc actaagccaa cgcccgccca taaattgggg ctgatctcca
     8041 gattgtcgcg cttgccgtta tgtaacgccg ccattcgctg ttgtcctacg gaatccgtcc
     8101 gggcgaatgc ggcctgtgct ttggcgatag tttcatcatc aagatgcgag attaaccgct
     8161 cggcggcctg ccacgcttcg tcgttagttt cacgaacaat cacatgcaga cgaataccga
     8221 aacgaatttt gcgtccatgc gcggcagctt tcgcccgcac ttgttcgatc ttctctttta
     8281 ctagttctgg cggttcgccc caggtgaggt agagatcaac ttgttctgcc gccagctctt
     8341 gggcgacgtc tgacgatccg ccaaagtaaa gcggaggata aggctgttga atcggcggga
     8401 agagcagttt tgctccgcgc acatgaatat gtttaccgtt gaaattgacg gtttccccca
     8461 gcaataagcg ccgccagacc tgggtaaatt ccgccgaggc ttcgtagcgc tcgctatgat
     8521 caaggaacac gccgtcgcct gccagctctt gtggatcgct gcctgtgacc agattaaaca
     8581 acgcacgacc atttgagaga cggtcaagcg tggcggcctg gcgagcggca acggtaggtg
     8641 aggttacgct ggggcgcagg gcgacaagaa acttcagccg ctgcgtcacc gggatcatcg
     8701 acgccgccac cagccacgca tcttcacacg atcgccccgt tgggatcagc acaccggtat
     8761 agcccagacg atccgccgct tgcgcaattt gttgcagata gccgtgatca accgggcgtg
     8821 aaccttcttc cgttccaaga taatgcccgt caccatgggt cggtaaaaac cagaacatat
     8881 tcagactcat aattgttttc cttccagttg agtgggctgc cagatgcgct ggcgaatatc
     8941 gacttttttc ggcaccagac gattttcata aaacagatct gccgtttgct gttgtaaggc
     9001 ggcaacctcg gcgttaaccg gtttgatggt ggtaggaggg cgatgatcta agtaagaggc
     9061 aatcaccggt gccggtaagc ccatcgtttt tgccagtaaa gcgatgcttt gctcgcgctg
     9121 gctgcgggtt aacgcatcgg cctcactaaa ggttgcgagt acgccctgaa taaaagcgcc
     9181 gtttttttct gcatagggac gagctgccag ataaaacgat ccagtttgat tgagatcggt
     9241 gccgtctttc agcacccgca cgccgccctg taataatgca gcagagtagt agggatccca
     9301 gatagcccag gcgtcaacgt taccttgctg gaacgcggcg cgggcatcag cgggcgtcag
     9361 gtaagtgggt tggatatcgg taaacttaag tccggcctga cgcagtgcac gcagtaaaag
     9421 gttgtgtgaa ctggaacctt tctgaaaggc aactttgtga cctttaagat cggctacggt
     9481 tttgatcggg ctgttttctg ccaccagaat cacttcggct ttgggcttcg gtggctcgac
     9541 gcccacatat accaaatcag ccccggcagc ctgggcaaag attggcggaa tatccccggt
     9601 actgccgaga tcaatactgc caacgtttaa cgcttccaac atttgcggac ccgcggggaa
     9661 ctccacccag gagatttttg attgcggata gcgtttttcc agtaactggt ggctttttgc
     9721 cagtaccata ccaatactgc ctttctgata gcctatacgt aacgcttcag gcgaggattc
     9781 tgcagcaacc gcaatcgtag agacgctaag caatcccacc agcgccagtt taatgatgtt
     9841 acgcatgggc attacctcgc agagacagaa ggtcaggaac ctgaacatcg cggcggtgca
     9901 atgcctgcca gaaagtttct agcgcggtat caagacgggt ttgcagattt ggcgtgaact
     9961 ggggtctgtg atggtaatca attacttgtg agtcatcggc aaacacgccg tgcaggatct
    10021 cctgagcttt cagtgcgctt aaaactggtt taagggcata atcgaccgcc agcagatggg
    10081 ccacggtacc gcccgtcgcc agcggtagca ccactttgcc ttgcaaagcg cgctctggca
    10141 gcaggtcgag cagggttttc agcgcgccgg aataggcggc tttatacaca ggtgtagcga
    10201 ctatcagtct atcagcctgt tgcagctgtt cggtgaaggt cttgagtgcc ggactatcga
    10261 aacgagcata aagcagatct tccggggcga agttttgcag attccagtga taaacctcta
    10321 catcgaggcc atttagtttt tcccgcgcat attccagcaa ggagctggag cgagaaggaa
    10381 agcgaggact acccgccagg gtgatgacac gcatactctc tccttataac caattgttct
    10441 ttttttgtta acattgataa caattcggtc agtctgtcgg agagacaaga aaatgccaaa
    10501 tataaatttt gtgtatcttt ttctgaaaat aaactaaaga aagggcttta tatatcacgc
    10561 atatttattt gttggatagt cattagatgt tgaatcaatg aattactcgt tcagttaata
    10621 tttaaatatt catgaaatct ataaattaaa gatttgtcac ttattggatt tagtatgctt
    10681 gatttaaatg gcggtcaatt attgttatta ttaagtctaa tgtcatttaa aggcattatc
    10741 atatttacga agagatcagg atgatgacga tgaaaaaaag tgtattgacg gcgtttataa
    10801 ctgtggtatg tgcaacgtcc agcgttatgg cagctgatga taatgctatc acggatggca
    10861 aagtaacatt taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag
    10921 attcagtagt cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct
    10981 ctggcgttaa aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa
    11041 atgcgacgtt tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc
    11101 aggctaccac cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta
    11161 catcggtaac tcccggtacc gaaacgtcga atattcagtt agcggattct gctgagcaaa
    11221 cagtgacctt taaagttgat tatatcgcta cggggaaagc gacttctggt aatgtgaatg
    11281 cggtaacaaa tttccatatt aactattatt aatagaactc attaattgtt ttattaatta
    11341 gtacccctct agtgttctgg aggggatatt catatttttt aagagtgact atttatgaaa
    11401 acttgcataa caaagggaat tgtgaccgta agtttaacgg caatattact cagctgctca
    11461 tccgcatggg cggcaggtaa aggcgggatt ggacttgcag caacacgtct tgtttattca
    11521 gaaggtgaag agcaaatttc actgggtgta cgtaacacca gtccggatgt tccttatctt
    11581 attcagtcat gggtgatgac cccagataat aaaaaatcag cagattttat tattacacca
    11641 ccgctatttg tgctgaatcc ggctaatgag aatctgttac gcattatgta cattggtgcg
    11701 ccgttggcga aagacagaga aacccttttc ttcactagcg tacgggcagt cccttcaaca
    11761 acgaagcggg aagagggaaa taccctgaag attgccacac aaagcgtcat caaacttttc
    11821 tggcgaccaa aaggtttagc gtatccctta ggcgaggctc cggcgaaact gcgttgcact
    11881 tcgtcagctg acatggttac ggtcagtaac ccaacacctt atttcattac cctgacagac
    11941 ctgaaaatag gtggaaaagt agttaaaaat caaatgattt ccccctttga taaataccaa
    12001 ttttctctgc caaagggggc caaaaatagc agcgtaacgt atcgaaccat caatgactac
    12061 ggggcggaaa cgccgcaact caactgtaaa tcgtaagccg tcttcagtta agagagcgag
    12121 atgtatagaa ctcatcgaca acacagcctg ttaagctctg gtggagtgcc atcgtttatt
    12181 ggtgggctgg tggtgtttgt gtcggcagcg ttcaatgcac aagctgaaac ctggttcgat
    12241 cctgcttttt tcaaagatga tccctcaatg gtggccgatt tgtctcgttt cgaaaaagga
    12301 caaaaaataa cgccaggggt ttatcgtgtc gatattgttc tgaatcagac aattgtagat
    12361 acgcgcaacg tcaattttgt tgagataacg ccagagaagg ggattgccgc ctgtttgacg
    12421 actgaaagcc tggatgcaat gggtgttaat actgatgcgt ttccggcttt taaacaactg
    12481 gacaaacaag cgtgtgtgcc attggcggag attattccgg atgccagcgt aacgtttaat
    12541 gtgaataaac tccgtctgga aatttcagta ccgcaaatcg ccatcaaaag taatgcccgt
    12601 ggttatgtcc cccctgaacg ttgggatgaa gggatcaacg cgctattact gggatattcg
    12661 tttagcgggg ctaacagtat tcatagcagc gcagacagtg attctggcga cagctatttt
    12721 ctgaatttaa acagtggcgt taatttaggc ccatggagat tgcgcaacaa ttcaatatgg
    12781 agtcgcagta gtggccaaac cgcagaatgg aagaatctca gcagctattt gcagcgggcg
    12841 gttattccac taaaaggcga actgaccgta ggtgatgatt atactgcagg cgattttttc
    12901 gatagtgtca gctttcgtgg tgtgcagctg gcgtcagatg acaacatgct gccagacagc
    12961 ctgaaagggt ttgcgcctgt ggtgcgtggt atcgccaaaa gcaatgccca gataacgatt
    13021 aagcaaaatg gttacaccat ttaccaaact tatgtatcgc ctggtgcttt tgaaattagt
    13081 gatctctatt ccacgtcgtc gagcggtgat ttgttagttg aaatcaaaga agcggacggc
    13141 agcgtcaata gctacagcgt accgttttcc agcgtgccat tactccagcg tcaggggcga
    13201 atcaaatacg cggtgacact ggcgaaatac agaaccaata gtaatgaaca gcaggagagc
    13261 aaatttgccc aggccacgtt gcagtggggc ggaccgtggg gaacgacatg gtatggtggt
    13321 ggacaatatg ctgaatatta ccgtgccgcc atgtttggtc tgggatttaa ccttggcgat
    13381 ttcggagcaa tttcgttcga tgtgacccag gcgaagagta cgctggcaga ccaaagcgaa
    13441 cataaaggtc agtcatatcg ttttctgtat gccaaaacgc tcaaccaatt gggcactaat
    13501 tttcaattga tgggctatcg ctattcgacg tcgggtttct acaccctttc cgacaccatg
    13561 tataaacata tggatggcta cgaatttaat gacggtgatg atgaagatac gccgatgtgg
    13621 tcgcgttatt acaatttgtt ttacaccaaa cgtggcaaac tgcaggtcaa tatctcccag
    13681 caattaggcg agtacggttc gttttattta agtggtagcc agcaaactta ctggcatacc
    13741 gatcaacagg atcggctatt acagtttggc tacaacacgc aaattaaaga tctctcgctg
    13801 ggggtttcct ggaactacag taagtcccgt ggtcaacctg atgctgatca ggtgtttgca
    13861 ctaaattttt ccctgccgct caatctgttg ctccccagaa gtaatgatag ctataccagg
    13921 aaaaaaaatt acgcctggat gacctctaac accagtatcg ataacgaagg gcacattaca
    13981 caaaacctgg gtttaacgga gacactactc gatgacggta acctgagcta cagcgtgcaa
    14041 cagggatata acagcgaggg gaaaacggct aatggtagcg ccagcatgga ctacaaaggg
    14101 gcgtttgcag atgcccgagt gggctacaac tacagcgata acggcagtca acaacaactg
    14161 aactacgctc tttcaggcag tttagttgcc cattcactgg gcattaccct ggggcaatcg
    14221 ctgggggaaa ctaacgtcct gattgcagca ccaggcgcag agaatactcg tgtggcgaac
    14281 agcaccgggc tgaaaactga ctggcgcgga tataccgttg tcccctatgc cacttcttat
    14341 cgggaaaatc gaatcgcact tgatgcggca tcgttaaaac gtaacgtgga tcttgaaaat
    14401 gcagtagtca acgtggttcc caccaaaggg gcgttggttc tggcggagtt caatgcccat
    14461 gcgggagcaa gggtattaat gaaaacatca aagcagggta taccgctgcg ttttggcgcg
    14521 atagcgacgc tggacggcat acagactaat agcggcatta ttgatgatga tggctcgctc
    14581 tatatgtccg gtttgcccgc tcagggagcg ataactgtac gctggggcga agctcccgat
    14641 caaatttgtc atatcagtta ccagcttacc gaacaacaaa ttaactctgc gattacgcga
    14701 atggatgcca tatgcagata atctttggag aaaaatgcgt gttattacta cgactatttt
    14761 ttgccgccgt cttaatgcta tggtgcgctc aaactgctgc ttatagcggg cagtgtcata
    14821 ccactcaggg gaatccgtat attggcgtca attttggcgt taaaaccctg gaggaagaag
    14881 aaaatacggc tggagtagtt aaagacaaat tttatcagtg gaacgaatcg aatgattatt
    14941 atgtttcctg tgattgcgat aaagacaatg tcagaagtgg ccgatgggca ttcgccgcgg
    15001 attcaccgtt agtctattta ggcgacaact ggtacaaaat taatgactat cttgccgcca
    15061 aagttttatt gcaggttaaa ggcagttctc ctactgcggt tcctttcgaa aacgtgggca
    15121 caggggcaga tacacgatgg catatttgcg atcccggcgg tcaacgttta ggtggccagg
    15181 gggctagcgg taatagcggt agcttttccc tgaaaatatt gcagccgttc gttggttcgg
    15241 tcgtcattcc tcctatggcg ctggcgcgat tatttgaatg ctacaacata cccgcaggtg
    15301 attcctgcac gactacaggt acaccggttt tagtgtatta cctgtctggt actatcaatt
    15361 cacttggctc atgttccgtc aatgccggag aaacaatcga ggtcgatctg ggcgacgtat
    15421 ttgcggctaa ctttcgtgtt gtagggcata agcctcttgg ggccagaacg gcagaacttg
    15481 caattccagt caggtgtaac acgggaaacg cggggttagt taacgtcaac ctgagtctga
    15541 cggcaaccac agaccccagc tatccccagg cgattaagac gtcacgtcct ggcgtgggcg
    15601 tggtggtgac cgatagccag aacaacatta tttcccctgc tggtggaaca ttaccgctct
    15661 ctattcctga tgatgcagac agtatcgcgc gaatgaatgt ctatccagtc agcacgacag
    15721 gtgtaccacc agaaaccggg cgatttgaag ccacggcaac ggtgagaata aattttgatt
    15781 aacgcgtgaa cgtatgaaga aaaaaacgat atttcagtgc gttatcttgt tctttagcat
    15841 tcttaacatc catgtcggga tggctgggcc tgaacaagtt agtatgcata tttatgggaa
    15901 tgtggtcgat cagggctgtg atgtcgccac caaaagtgca ttacaaaata ttcatattgg
    15961 tgattttaat atcagtgatt ttcaggccgc gaataccgta agcactgctg ctgatttgaa
    16021 tatcgatatc accggttgtg ccgctggtat tactggcgcg gacgtccttt ttagcggcga
    16081 ggctgacacc cttgcgccga cactgctcaa actaactgac acaggcggaa gcggtggtat
    16141 ggcaacgggg attgccgtgc aaattcttga tgcgcaaagt cagcaagaaa tcccgctcaa
    16201 tcaggtccag cctcttacgc ccttaaaagc cggggataac acactcaaat atcaacttcg
    16261 ttataagtcc acaaaggcgg gagcaacggg cggtaatgcg acggcggttc tctattttga
    16321 tctggtttac cagtgaagga gtgatgttga aacggataat ctggattctg ttcttactgg
    16381 gattaacggg gggctgtgag ctatttgccc atgatggcac agtcaacatt agcggatcgt
    16441 ttcgccgtaa tacatgcgtg ctggcacagg atagcaagca aatcaatgtg cagttaggcg
    16501 atgtctcgct gacacgtttt tttcatggga attatggccc ggagaaatct ttcatcatta
    16561 atttgcagga ttgcggaact gatgtcagca cggttgacgt caccttttca ggaacccccg
    16621 acggagtgca gagcgaaatg ctgtccattg aaagtggtac ggatgcagcc agcgggctgg
    16681 caattgcgat tctggatgat gcgaagatat tgattccgct taaccaggcg agtaaagact
    16741 acagcctaca tagcggtaag gtgccattaa ctttttatgc gcaattgcga cctgtcaata
    16801 gcgatgtgca gtcgggtaaa gtgaatgcca gcgcgacatt tgtacttcat tatgactaat
    16861 acgtggaaca gattggcgct cttgattttc gccgttttat cgctgctggt ggcgggtgaa
    16921 ttgcaggcag gagtggtggt cggcggaacg cgatttatct ttccggcaga cagagaatcg
    16981 atatctattt tactgactaa taccagtcag gaatcctggc ttattaacag caaaatcaac
    17041 cgcccaacgc gttgggccgg gggggaagcg tcgacagtgc cagcaccatt actggccgct
    17101 ccgccactta ttctcctgaa gcccggtact acaggcacgt tgcgcttgct gagaacggaa
    17161 agcgacatct tgcctgtgga tcgcgaaacg ctatttgagt taagcattgc cagcgtgcca
    17221 tccggcaaag ttgaaaatca gagcgtaaaa gtggcgatac gctcggtatt taaactgttc
    17281 tggcgacccg aaggtttgcc gggcgacccg ctggaagctt atcaacaatt acgctggaca
    17341 tggaattcgc agggtgtaca actcactaac ccaacgcctt actacattaa cctgattcag
    17401 gtgagcgtaa atggtaaagc gttaagtaat gcgggagttg tgccgcctaa aagccagcgt
    17461 cagacaagct ggtgtcaggc aatcgcaccg tgtcatgtcg cctggcgggc gataaatgat
    17521 tacggcgggt tgagtgcaaa gaaggagcaa aatctgccct gaaacaggtt cggaaaacgt
    17581 ttgcgttttt tttgccgcag gtcaattccc ttttggtccg aactcgcaca taatacgccc
    17641 ccggtttgca caccgggaat ccaggagagt tcatgtacta ccccttcgtt cgtaaagccc
    17701 ttttccagct cgatccagag cgcgctcatg agtttacttt tcagcaatta cgccgtatta
    17761 caggaacgcc gtttgaagca ctggtgcggc agaaagtgcc tgcgaaacct gttaactgca
    17821 tgggcctgac gtttaaaaat ccgcttggtc tggcagccgg tcttgataaa gacggggagt
    17881 gcattgacgc gttaggcgcg atgggatttg gatcgatcga gatcggtacc gtcacgccac
    17941 gtccacagcc aggtaatgac aagccgcgtc tctttcgtct ggtagatgcc gaaggtttga
    18001 tcaaccgtat gggctttaat aatcttggcg ttgataacct cgtagagaac gtaaaaaagg
    18061 cccattatga cggcgtcctg ggtattaaca tcggcaaaaa taaagatacg ccagtggagc
    18121 agggcaaaga tgactatctg atttgtatgg aaaaaatcta tgcctatgcg ggatatatcg
    18181 ccatcaatat ttcatcgccg aataccccag gattacgcac actgcaatat ggtgaagcgc
    18241 tggatgatct cttaaccgcg attaaaaata agcaaaatga tttgcaagcg atgcaccata
    18301 aatatgtgcc gatcgcagtg aagatcgcgc cggatctttc tgaagaagaa ttgatccagg
    18361 ttgccgatag tttagttcgc cataatattg atggcgttat tgcaaccaat accacactcg
    18421 atcgttctct tgttcaggga atgaaaaatt gcgatcaaac cggtggctta agtggtcgtc
    18481 cgcttcagtt aaaaagcacc gaaattattc gccgcttgtc actggaatta aacggtcgct
    18541 taccgatcat cggtgttggc ggcatcgact cggttatcgc tgcgcgtgaa aagattgctg
    18601 cgggtgcctc actggtgcaa atttattctg gttttatttt taaaggtccg ccgctgatta
    18661 aagaaatcgt tacccatatc taattatttc ttcgaattcc tctttataag ataatcaggg
    18721 ctttattttc agccctggtt gttttatatt cattgctgtt gcttatttag acattttgta
    18781 cttttattgt tgaggttatt aagcgaagcg acaatggatt gtgttgctgc ggttttatag
    18841 ggtaggggag aggcagatgc gaattaaacc agacgataac tggcgttggt attacgatga
    18901 agagcacgat cgaatgatgc tcgatttagc caatggtatg ctatttcgct cacgttttgc
    18961 gcgcaagatg ttgactccag atgccttttc tcccgcaggc ttttgcgttg acgacgccgc
    19021 gctctatttc tcttttgaag aaaagtgccg cgattttaat ttatctaaag aacaaaaagc
    19081 cgaactggtg ctgaatgcac tggtagcaat tcgctatctc aaaccgcaaa tgcggaaaag
    19141 ctggcatttt gtttcccatg gtgaaatgtg ggtgcccatg ccgggcgatg cggcctgcgt
    19201 ttggttgagc gatactcacg agcaggtcaa tttgctggtc gttgaatctg gcgaaaatgc
    19261 cgcactatgc ctgctggcac aaccctgcgt tgttattgcg ggtcgcgcga tgcagttggg
    19321 cgacgccatt aaaatcatga acgacaggct gaaaccgcag gttaatgttg acagcttcag
    19381 cctcgaacag gcagtctaac gcgccaactt aagtgcagtc ttcggtacac agctacagca
    19441 aagaatggtg ccatcatcgc ccattgctga ttttttcagc ggcgtgactt cgccttctaa
    19501 aagctgaaca cggcaacttc cacaaatgcc cgcgcggcaa gaataaggga tacgaattcc
    19561 ctgattttct aattgctcca gcaacacctg ttggttattt ccacgaaatg cctgtccctg
    19621 ccagtcaata tctacatttg cgtccggttg ttgcgtgatg ttggcggtat catcagcggc
    19681 agctgcgccg taaatttttg ccggagccgt tgccagaatt tccacctcat cgccaacgcg
    19741 aatcacgccg ctattacggg caattaaatt ctgaccaaaa tcgacatcgc cgttatcctg
    19801 ggcagtgcgg aaagattgca atgtttttaa tggttcgcct gccggatgtt tttgcccttt
    19861 ttctgggctg acggtggtga aaatacagcg gctacaaggt ttaaccacat caaacaccac
    19921 atcaccaatg cgaatcactt tccagcgatc ttcttcccag gctgacgcgc cggaaaccac
    19981 cagattgggg cggaattgct ccatttttac actggccgga caacgttgtt ggagatcacg
    20041 taacgaggct tcgttagcaa gaaggtaagg atagccatca gcaaatgaca gaggtacagt
    20101 gttgtggcgt ttcacgcgcc gggtcatttg tggccccacc cagcgtaatt gcacttcgcg
    20161 ggagaaaaat ccacttagcc atttgttgat cgcgtctggc gcaattcgcg cggtaaaatg
    20221 tgtgccccaa acttcggttg gtgcgtcttg tgtggcgaaa tcagcaaaac gaacatatgc
    20281 gctactgcca tctggtgcgg tgagatgcaa gccatcatgc acaggtgaag gagtaaaccg
    20341 taccatctgg ggaaactggc gagcggtaat aaacgtaccg tcaggttccg tgatcataaa
    20401 gatgcgatcg aaggccagac cactgacatc agccagagca tgtgtaagac caatgccgcg
    20461 catcgattta acaggatgaa taaaaagccg ggttaatgtc gccacggtgc ggtcctcaaa
    20521 tgaaaataag ccctcaactt tatgacatga cgcgcttatt agctataatg cgcaacaatt
    20581 ttcttagcga ctgtcccatt aggctatttt atttgtcagt ttggccttgg gttgtgctcg
    20641 aaatcctcac gtacttgtgt acgctccggt ttctccgcgc tgtccatgtc cagactgact
    20701 gcaacaatta cgcctactgc ggtaggctct tagagtaaaa gtgacgatat gaattctctg
    20761 tttgccagta cggcccgtgg gctggaagag ctgttaaaaa ctgaactgga aaacctgggg
    20821 gccgttgaat gccaggtggt tcagggtggg gtccatttca agggcgacac acggcttgtt
    20881 taccagagcc tgatgtggag ccgcctggcc tcgcgtatta tgttgccgct gggcgagtgt
    20941 aaggtttaca gcgatttaga cctctatctc ggtgttcagg cgatcaactg gacagagatg
    21001 tttaatcctg gcgcgacctt cgctgtccac ttcagtggtt tgaatgacac catccgcaac
    21061 agtcagtacg gcgcgatgaa agtgaaagac gcgatcgtcg atgctttcac gcggaaaaat
    21121 ctgccgcgtc caaatgttga tcgcgatgcg ccggatatcc gcgttaacgt ctggctgcat
    21181 aaagaaaccg ccagtatcgc tcttgatctg agtggtgatg gtttacatct gcgtggctat
    21241 cgcgatcgtg ctggtattgc gccgatcaaa gaaaccctgg cagccgctat tgtgatgcga
    21301 tccggctggc agccaggaac accgctgctc gatccgatgt gtggttccgg tacgttgctg
    21361 attgaagcgg cgatgctagc gaccgatcgc gcaccaggct tgcaccgtgg gcgttggggc
    21421 tttagcggct gggtgcagca tgatgaagat atctggcagg aagtgaaagc ggaagcgcaa
    21481 actcgtgccc gtaaaggcct ggctgagtat agctctcatt tttacggttc ggacagcgac
    21541 gcacgggtga ttcaacgtgc gcgcactaac gcccgtcttg cggggattgg tgaactgatc
    21601 acctttgagg tgaaagatgt cgcgcaactg accaatccgc tgccgaaagg gccgtacggt
    21661 acagtgttga gcaacccgcc atacggggaa cgtctggaca gcgaaccggc gctgattgcg
    21721 ctgcatagcc tgctgggtcg gatcatgaaa aaccagtttg gtggctggaa tctctctttg
    21781 tttagtgcct cgccggatct gctaagctgc ctgcagctgc gtgcagacaa acagtacaag
    21841 gcgaaaaacg gcccgctgga ctgcgtacag aaaaattacc acgttgccga aagcactcca
    21901 gacagcaaac cggcgatggt agcggaagac tacgccaacc gcctgcgtaa gaacctcaaa
    21961 aaattcgaga agtgggctcg tcaggaagga attgaatgtt accgcctgta tgacgccgat
    22021 ctgccggaat ataacgttgc cgttgaccgt tatgccgact gggtggtggt gcaggagtat
    22081 gcaccgccaa aaactattga tgctcacaaa gcgcgtcagc gtctgttcga tattatcgct
    22141 gcaaccattt cggtactggg gattgcgcca aacaaactgg tgctgaaaac ccgtgaacgt
    22201 cagaagggca aaaatcagta ccagaaactg ggcgagaagg gcgagtttct ggaagttacc
    22261 gaatataacg ctcacttgtg ggtgaatctg acggattacc tcgataccgg tctgttcctc
    22321 gatcaccgta tcgcccgtcg tatgctcggc cagatgagca aaggcaaaga tttcctcaac
    22381 ctgttctctt ataccggcag cgccaccgtg catgcgggat taggcggtgc acgcagcacc
    22441 accaccgtgg atatgtcgcg tacttatctg gagtgggcag aacgcaacct gcgtctgaat
    22501 ggcctgaccg ggcgtgcgca tcgcctgatt caggccgatt gcctggcgtg gctgcgtgag
    22561 gcaaatgaac agttcgatct gatctttatc gatccgccaa ccttctctaa ctcaaaacga
    22621 atggaagatg cgtttgatgt tcagcgcgat catctggcgc tgatgaaaga tttgaaacgt
    22681 ctgctgcgtg caggtgggac gatcatgttc tcgaacaata aacgtggctt ccgtatggat
    22741 ctcgacggac tggcaaaact gggactgaaa gcacaagaaa ttacgcaaaa aacgctctcc
    22801 caggatttcg cccgtaaccg ccagatccac aactgctggc tgattaccgc agcctgaaag
    22861 gaatagtaat gtcattaatc agtatgcatg gcgcatggct gtcgttcagc gacgcgccgc
    22921 ttctcgataa cgcagaactg catatcgaag ataacgaacg tgtttgtctg gtaggccgta
    22981 acggcgcggg caaatcgacg ttaatgaaga tcctcaaccg tgaacagggg ctggatgacg
    23041 ggcgcattat ttacgagcaa gatttgatcg tggctcgtct gcaacaggat ccgccgcgta
    23101 acgttgaggg tagcgtttat gatttcgttg ccgaaggcat tgaagaacaa gcggaatatc
    23161 tgaaacgcta tcacgatatt tcgcgcctgg tgatgaacga cccgagcgag aaaaatctca
    23221 acgaactggc gaaggttcag gaacagctgg atcaccacaa cctgtggcag ctggaaaacc
    23281 gcatcaacga agtgctggcg caactggggt tagatcctaa cgttgcactg tcgtcgcttt
    23341 ccggcggctg gttgcgtaaa gcggcattag gacgcgcgct ggtgagtaat ccgcgcgtgc
    23401 tgttgcttga tgaaccgact aaccacctgg atatcgaaac catcgactgg ctggaagggt
    23461 tccttaaaac cttcaatggg acgattattt tcatctccca cgaccgttcg tttatccgca
    23521 atatggcgac gcgcattgtc gatctcgatc gcggcaagct ggtgacctat ccagggaatt
    23581 acgaccagta cctgctggaa aaagaagaag ccctgcgcgt ggaagaactt caaaatgccg
    23641 agttcgatcg caaactggcg caggaagagg tgtggatccg ccaggggatc aaagcacgcc
    23701 gtacccgtaa tgaaggccgc gtacgcgccc tgaaagcaat gcgtcgtgag cgtggcgaac
    23761 gtcgcgaagt gatgggtacc gcaaagatgc aggtggaaga ggccagccgc tccggtaaga
    23821 tcgttttcga aatggaagac gtttgctacc aggttgacgg taagcaactg gtgaaagatt
    23881 tttcggccca ggttctacgt ggcgacaaaa ttgccctgat tggtccgaat gggtgcggca
    23941 aaaccacgct gctgaaacta atgctcggtc agcttcaagc ggacagcggg cgtattcacg
    24001 ttggcaccaa actggaagtg gcttatttcg atcagcaccg cgcggaactg gatcccgata
    24061 aaacggtgat ggataacctt gccgaaggta agcaagaggt gatggttaac ggcaagccac
    24121 gccacgtatt gggctatttg caggactttc tgttccatcc gaaacgggcg atgacgccgg
    24181 tacgtgcgct ttctggcggt gagcggaacc gcttgctgct ggcgcgtttg ttcctcaaac
    24241 caagcaactt attgattctt gacgaaccga ccaacgatct tgatgtcgaa acgctggaac
    24301 tgctggaaga actgatcgac agctatcagg gcacggtatt gctggttagc cacgatcgtc
    24361 agtttgtcga taacaccgtt acagaatgtt ggatcttcga aggcggcggt aaaattggtc
    24421 gttatgtcgg cggttatcat gatgcccgtg gtcagcaaga gcagtatgtg gcgctcaaac
    24481 agcctgcggt gaaaaaaacc gaagaagccg ccgcggcaaa agcagaaact gtaaaacgca
    24541 gcagtagcaa actaagctat aaattgcagc gcgaactgga gcagctaccg caattgctcg
    24601 aagatctgga ggcgaagctg gaagccctac agacgcaggt ggcggatgct tccttcttca
    24661 gtcagccaca tgagcagaca caaaaagtgc tcgccgatat ggctgctgca gagcaggagc
    24721 tggagcaagc ctttgaacgc tgggagtatc ttgaagcgtt aaaaaatggt ggctgatcgc
    24781 aaaatagtcg atttaaacag agacagctga caacggtaaa attgcctgat gaactgggtt
    24841 tatcaggcat gaaaaccaac actttgttag tcatctgtaa aatagcgcat cattaaggag
    24901 taccaatgtg cgaacatcat catgccgcga agcacatcct gtgctcgcag tgtgacatgc
    24961 tggtggcgtt accgcgcctt gagcatggtc agaaagcggc atgtccccgg tgtggcacaa
    25021 cgttaaccgt ggcgtgggat gcccctcggc agcgtcccac cgcctatgcg ttggctgcac
    25081 tgttcatgct gttgctgtcc aacttgtttc cttttgtgaa tatgaacgtt gcaggagtta
    25141 ccagtgaaat tacattactg gaaattcccg gcgtgctttt ttctgaggac tacgccagcc
    25201 tcggcacctt tttcctgttg tttgtgcaac tggttcccgc gttttgtctg ataaccattc
    25261 tgttactggt gaatcgcgcg gaattaccgg tccgtttaaa agagcaactg gcacgggtgc
    25321 tttttcaact caaaacctgg ggaatggcgg agattttcct cgctggtgtg ctggtcagtt
    25381 tcgttaaact gatggcttac ggcagcattg gggtaggcag cagctttctc ccctggtgtt
    25441 tattttgtgt cctgcaactg cgcgcttttc agtgcgttga tcgtcgctgg ttatgggacg
    25501 acatcgcccc gatgccagaa ctgcgccagc cgctaaaacc aggcgtcacg gggatacgtc
    25561 aggggctgcg ttcgtgctcc tgttgtacgg caatccttcc tgctgatgaa cccgtgtgcc
    25621 cgcgttgtag taccaaaggg tacgttcggc gtagaaacag cctgcagtgg acactcgcgc
    25681 tgcttgtaac gtccatcatg ctgtatcttc cggctaatat tttgcccatc atggtgacgg
    25741 atttattagg ctcgaagatg ccgtcgacga ttctcgctgg ggtcattctg ttatggagcg
    25801 aaggctctta tcccgtcgct gcggtgatct ttctggccag tattatggtg ccaacgttaa
    25861 agatgatcgc catcgcgtgg ctgtgttggg atgccaaagg gcatggcaag cgcgacagtg
    25921 aaagaatgca tttgatttat gaagttgttg agtttgtagg ccgctggtcg atgattgacg
    25981 ttttcgttat cgcggtgctc tcggcgctgg tgcgtatggg aggtttaatg agtatttatc
    26041 cggcaatggg tgcattaatg tttgctttag tcgtcataat gacaatgttt tctgctatga
    26101 cgtttgaccc gcgtttgtcg tgggatcgtc aacctgaatc agagcatgag gagtcctgac
    26161 agtatggaat ctaataatgg ggaagccaaa atccagaaag tgaagaactg gtctcccgtg
    26221 tggatatttc ctatcgtcac ggcgctcatt ggggcctggg ttctttttta tcattacagc
    26281 catcagggac cggaagtgac cctgatcacc gcgaatgcgg aaggaattga aggtggcaaa
    26341 accaccatta aaagccgtag cgttgacgtc ggcgtggttg aaagcgccac actggctgat
    26401 gatttgacgc acgttgaaat caaagcgcgg ctgaattccg gtatggaaaa attgctgcat
    26461 aaagacaccg tcttttgggt ggtgaaaccg cagattggtc gcgaagggat tagcggcctg
    26521 ggaacgctgc tgtctggagt ttatatcgaa ctgcagccag gcgcgaaagg cagcaaaatg
    26581 gataaatacg atttgctgga ctcgccaccg ttggccccgc ctgatgcgaa aggtatccgt
    26641 gtgattctcg atagcaaaaa agccgggcag ctctcgccag gagatccggt gctgttccgt
    26701 ggctatcggg taggttcggt tgaaaccagc accttcgata cacaaaaacg caatatcagc
    26761 tatcaactgt tcatcaatgc accttatgac cgactggtga ccaacaatgt tcgcttctgg
    26821 aaagatagtg gcattgcggt tgatctgacg tcagcaggga tgcgtgtgga gatgggctca
    26881 ttgacaacgc tgctgagtgg cggtgtcagc tttgatgtgc cggaaggtct ggatttaggg
    26941 cagccagtgg caccgaaaac agctttcgtt ttgtatgatg atcagaagag cattcaggat
    27001 tcgttgtaca ccgatcacat tgattatctg atgttcttta aagattcggt acgcggtctg
    27061 caaccgggag ctccggtaga gttccggggt attcgcctgg gtaccgtaag caaagtgcca
    27121 ttctttgcgc cgaatatgcg tcagacattt aacgatgatt accgtattcc ggtactgatt
    27181 cgtatcgagc cagagcggct gaaaatgcag cttggcgaaa atgcggatgt tgttgagcac
    27241 cttggcgaat tgttgaaacg tggtttacgc ggatcgctga aaaccggaaa cctggtcact
    27301 ggtgcactgt atgttgatct cgatttctat ccaaatacgc ctgcaataac cggtattcgt
    27361 gaatttaatg gttatcagat tatcccgacc gttagcggcg gcctggcgca aatccagcaa
    27421 cgactgatgg aagcgttgga taagatcaac aaactgccat tgaatccgat gattgaacag
    27481 gcaaccagta cgctttctga aagtcagcgc acaatgaaaa acctgcaaac gacgctggat
    27541 agcatgaaca agatcctcgc tagccagtcg atgcagcagt tgccgacgga tatgcaatca
    27601 acgttgcgtg aattgaatcg cagcatgcag ggcttccagc ctggctccgc agcctacaac
    27661 aagatggtgg cggatatgca gcgccttgat caggtgttgc gagaactgca accggtgctg
    27721 aaaacgctca atgagaagag taacgcgctg gtatttgaag cgaaggacaa aaaagatcca
    27781 gagccgaaga gggcgaaaca atgaaaaagt ggctagtgac gattgcagca ctgtggctgg
    27841 ccggatgcag ctccggcgaa attaataaaa actattacca gttacctgtg gtgcagagcg
    27901 gtacacaaag taccgccagc cagggcaatc gtctgttatg ggtagagcag gtcactgttc
    27961 ctgactatct ggcggggaat ggtgtggttt atcaaaccag tgatgtgaag tatgtgattg
    28021 ccaacaacaa cttgtgggcc agcccgttgg atcaacagtt gcgcaacacc ctggttgcca
    28081 acctgagtac gcaactgccc ggctgggtgg ttgcctccca gcctctggga agcgcccagg
    28141 acacgctcaa tgttaccgta acggagttta acggtcgcta tgatggcaag gtcattgtca
    28201 gtggtgagtg gctgttgaac caccagggac aactgatcaa acgtccgttc cgtctggaag
    28261 gagtgcaaac tcaggatggt tacgatgaga tggttaaagt actggccagt gtctggagtc
    28321 aggaagccgc ttctattgcg caagagataa agcgtctacc ttaattataa agatttgtaa
    28381 atataaccgt ctccggtatg ttgcctgagg cggttttttt gtttctaacg tgcggaaaaa
    28441 tttgttcctc ttcacatttt ttgtacaacc gacatgcccg tgtagctcac aaatatgaca
    28501 gtggcgtgaa ttttgcgcat tgacggcagt tatgattcgc ggtattgctt aactgtgatt
    28561 gcacatttag taatcactgt tttcttttcc accagaaacc agtatgaggg aaacgaggca
    28621 tgaagagaca aaaacgagat cgcctggaac gggcacatca acgtggttat caggccggca
    28681 tcgccggacg ctcaaaagaa atgtgtccct atcagacgct gaatcaaagg tcacaatggc
    28741 tgggaggctg gcgagaagcc atggcggaca gggtagtaat ggcctgattc tgtctcttta
    28801 aaaagaaacc tccgcattgc ggaggtttcg ccttttgata ctctgtctga ttataatcag
    28861 aaggcagacg tatcctggaa cagaccgact ttcaggtcgc tggcggtata gataagacga
    28921 ccatcaacca gcacttcgcc atccgccagg cccataatca gacgacggtt aacaatgcgt
    28981 ttaaagtgaa tacggtaggt cacttttttc gctgtcggca gtacctgacc agtgaatttc
    29041 acttcgccaa cgcccagcgc gcggccttta ccttcgccgc ccagccagcc gaggtagaac
    29101 cctaccagct gccacattgc gtccaggccc aggcatcccg gcataaccgg atcgccaata
    29161 aagtggcatc cgaagaacca cagatccgga ttgatatcca gttctgcttc aacataccct
    29221 ttgtcgaagt taccacccgt ttcggtcatt ttgaccacac ggtccatcat cagcatgttc
    29281 ggtgctggca attgcgggcc tttagcgcca aacagttcac cgcgaccaga ggcaagaagg
    29341 tcttcttttg tataggattc gcgtttatct accatgttct ctgtaagcct tattttattg
    29401 aagcacgcag gatagctaac acgtgtacgc tgaacaagtc cgatcagttc ggaataaacc
    29461 agttcagcca acgtaatggc catggaaaac ggtgacgtcc ttcctgttgc gatgcttgcg
    29521 cgatacgttc ctggatggtt tgcatcagcg tcgtttggcc ttcgccatcc cacaccagat
    29581 ttaataataa cggcagtgcg tcagtcacat cgtctactgc ccagatggtg aatttgcctt
    29641 cttctaccgc tttcaccagt tcactgtgaa gacttaaatg gcgaacgtta gctgtgggga
    29701 taatgacacc ttgtttcccg gttaactcac gttgctggca aatagcaaag aagccttcga
    29761 ttttctcatt taaaccaccg accggctggg cgcgaccgaa ctgatcgact gaacctgtga
    29821 tagcgatact ctgattcacc ggcacatcgg cgagggcgct tatcagggcg cagagttcag
    29881 ccatcgaggc actatctcca tcaacttcac tgtatgactg ctcaaatgtc agcgatgctg
    29941 agaaggggat ctgttgctca agctgtagtt ccgacatcag gaacgcttgc atgatcatca
    30001 tccctttcgc atggatattg ccgccaagct ccgctttgcg ttcgatgtcg gtgaattcac
    30061 catcgccaat atgcacaacg cagctaatgc gagaaggttc gccaaaagcg cgtggatgac
    30121 ccggaaattc aatgaccgaa agggcgttaa tttgcccgat gcgttcgcct tcggtttcaa
    30181 tcaggatttg ctcctgaagg atctcatcct gcatacgttc agcgaggaaa ccttcgcgcc
    30241 attcacgctg ctgcagcatt aagtttagct gctcgccgga gaaggtgtcg ccatcacaca
    30301 gggaggcgac ctctttacac tggcggagga tccactgcgg gctaagcgga agtgtttctt
    30361 gttcaccggt gtagcgtgct gcttcgcgga taagtaccgg ccaggcatcc gctcccggtg
    30421 caggtaagtg attatgtctg gcggtaaatg tcacccagcg acaccactgg gttactgact
    30481 ctgcatcgac aatctgcaga gtatcttcaa attcgctata aatagcctgc tctgaaagct
    30541 ctggctccat ctcctggaaa tcagccaatg attcgcgttc gcctaccaga atgactttca
    30601 gcttcaatgg catcgaaggc acagagacgg ggagagggcg cgactcatca aacgcaaccc
    30661 agtcaaaacg ctcgcggtta acgatatttt tcagccgcat ccacagcaga ggttgcgcca
    30721 gcagtgtacg caaagagatg atgagaatac cgccatttgc ctgatgcacc agaccaggct
    30781 gcagggtaat gtcgccatta aactggcgca ggcagccaaa gagttgctcc gcttctaccc
    30841 agtcggcagc gacaacttgc gttaaagtcg caaaattatc atctgcactc actgcgtgac
    30901 gtaagcggat ggagtggccg gaaacctcat agtgaccgcc caccagttgg cctgcatcgc
    30961 tttgtagcgt acgcgcggca ttggcaataa gattcagata ctcagactct tccggggcct
    31021 tcgccagcat aaaagaggag gatgctcgcg tatgcagcaa ctgctccagt gcgaattgca
    31081 gtcgcggttg agtatcactg aataaaggat cgttttcgtc aatcaaatgt ggctgagcaa
    31141 atatttcctg atagctatcg gtatcaggaa ccaggtcacg ccatgcaagt ttcgtaatgg
    31201 tcaaagttga tgttttttag tctgttgtca aagccgcgat tataccgtaa ccggcactac
    31261 agcacacgta gaaagcaccg acaatactcc tggcatgggc gttaaagctc acaggatgga
    31321 gattcttttc ttcactggcc taaaaagctg atattctgta aagagttaca cggtaacatt
    31381 gagatcgcta tgaaatatca acaacttgaa aatcttgaaa gcggttggaa atggaagtat
    31441 ctggtgaaga agcatcgcga aggggagtta atcacccgtt acatagaagc cagtgccgcc
    31501 caggaagccg tcgatgtgtt gctctcgctc gaaaatgaac ccgtgctggt aaatggctgg
    31561 attgacaagc atatgaatcc ggaactggtc aatcgcatga agcaaaccat tcgggcaaga
    31621 cgaaagcgcc attttaatgc ggaacaccag catacgcgca aaaaatcgat cgatctggaa
    31681 tttattgtct ggcaacgtct ggctggtctt gcgcagcgtc gcggtaaaac gctttctgaa
    31741 acgattgttc agctgattga agatgcggaa aacaaagaga aatacgcgaa taaaatgtct
    31801 tctctgaagc aggatctgca ggcattgctg ggtaaggaat aactgacgaa agtcagttca
    31861 atttactaaa ggcaaaaaaa accccgcagc agcggggttt ttctaccaga cgagaactta
    31921 agcttgcggc tgagttacaa cgtctttgat acctttaact tcgatctcta cgcgacgatc
    31981 cggagccagg cagtcgatca gtgcagcacg ctgtttcacg ttgtcacagg tgttgccagt
    32041 aaccgggttg gattcgccca taccacgtgc ggagatcttg tctgccggga tacctttgga
    32101 gatcaggtaa tcaacaacag actgagcacg gcgctcggac agaccctggt tgtaagcgtc
    32161 agaaccgatg cggtcggtgt aacccagaac aactacggaa ccgtctttcg gatccaggtt
    32221 gctcagctgg ctgtacagct gatccagagc agcctgacct tccggtttca gggttgcttt
    32281 gttgaagttg aacagaacgt cagacttcag agtgaagtgc ttggtctgta cttccggtgc
    32341 cggagctgga gccggagcaa ctactggagc tgcttcgccc tgaccgaaac ggtaggaaac
    32401 acccaggctc agcatgccgt tgtccggacg agtgccgatg gtgtgtgcgt caccgatgtt
    32461 gttggtccac tggtattcca gacgggtagc gatttcagga gtgatcgcgt actcaacacc
    32521 gccagcgaag accggagaaa cgccggtgtc gtggttttta ccataaacgt tggatttagt
    32581 gtctgcacgc cataccatac cacccagacg agtgtagatg tccaggtcgt cagtgattgg
    32641 gtaacccagt ttagcggtca gttgaacgcc ctgagctttg tatgcaccgt tttcaacgct
    32701 gcctttgtac ggcatacgac ctaaccagtc gtaacccatt tcaaagccaa catacgggtt
    32761 aacctggtaa ccaccaaaag caccagcgcc cagttggttt tcatgggtcg ggccattgtt
    32821 gttgatgaaa ccagtgtcat ggtactggga ccagcccagt ttagcaccag tgtaccaggt
    32881 gttatctttc ggagcggcct gcgctacggt agcgaaacca gccagtgcca ctgcaatcgc
    32941 gatagctgtc tttttcattt tttgcgcctc gttatcatcc aaaatacgcc atgaatatct
    33001 ccaacgagat aacacggtta aatccttcac cgggggatct gctcaatatt aactctaccg
    33061 atatcttcgg cttatgccga gcacccctgg cgatgtaaag tctacaacgt agttgaaaac
    33121 ttacaagtgt gaactccgtc aggcatatga aaaaaaagtc ttgtataagg tatgtttaat
    33181 cttttttgtc agcgacaatt tacagaagaa aatcgcggaa accgcttcag acaagcctcc
    33241 gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg acaaaaaaag ttccaggatt
    33301 aatcctaaat ttacttaatg atacaaatta gagtgaattt ttagcccgga aagttgtctc
    33361 gtggcgtgag aggatgcgct taccggacgc ataataaacc ccatagcgtt accttcattt
    33421 gccgcatcaa caagttcagc atgctcttct tcagtcaaat catctgccaa ccaaccgatc
    33481 accacactgt aattgcccgt gcgtaaagcg cgaaccattg actccacagt gtggcaaggg
    33541 gagagctggc taatctgcat tactttcgtt aagggtagcc cagatgcctg aacccattcc
    33601 cgactcagtt tttgttgcgg tgttaaccag agttgccagc gcgattgctg accgagttgc
    33661 tgtaacaatg gcaacagtag aagttgcgtc atcatgggct gatcttcgcg atagacaact
    33721 tcactgataa gcccggctgt agtgttttcc gtagagacac gcgcaatttt acttgctgcg
    33781 gatgagaacg acgaagaacg atgtgcatag cctgaagtgt acataatcaa tccagcccct
    33841 gtgagttact gtatggatgt acagtacatc cagtgacaac aaagatcaac cctattttcg
    33901 gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac ataaattaat cttgcccctt
    33961 aagaataagt tgcctatttt cgtagttaac ggatccgtta atgtgaatca ttcttttatg
    34021 ttatgatttt aaaaggaatt ttatgaaaag cctctcctat aagcggatct ataaatcaca
    34081 agaatacctg gcaacgttgg gcacaattga ataccgatca ttgtttggca gttacagcct
    34141 gaccgttgac gacacggtgt ttgcgatggt ttctgatggt gagttgtatc ttcgggcttg
    34201 tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc tggctgacat ataaaaagtg
    34261 tggccgatcc gttaccctca attactatcg ggttgatgaa agtctatggc gaaatcaact
    34321 gaagctggtg cgtctgtcga aatattctct cgatgcagcg ctgaaagaga aaagcacgcg
    34381 caatacccgg gaaagactga aagatttgcc caatatgtct tttcatctgg aagcgattct
    34441 cggggaggtg gggattaagg atgtacgggc gttacgtata cttggggcaa aaatgtgttg
    34501 gttgcgactg cggcagcaaa acagtctggt gacagaaaag attctgttta tgcttgaagg
    34561 tgccattatc ggcattcatg aagctgcgct cccggtggca cgccgccagg agcttgcaga
    34621 atgggctgac tctcttacgc cgaaacagga gtttcctgcg gaacttgagt aatctggcgt
    34681 tgcagacgac caatctcagg cagtaatgca atcaataatc caacttgttg tacgaccaga
    34741 ggttctttgc tgtctgcccg tggttcaagt tgctgcatcc gctgtttcag gctagctaat
    34801 gcctcattga cgcgttcttc atcagcaggt tgatgatgta acgcgtcatc aacatagcaa
    34861 actgcctcat caagaaacgc cagaatttca ggattagtta actgctcccg gtgagcaccg
    34921 agggctgaga tatagctggt aaacgtatgg ttaaggcaca gcaaccgaaa cgcggcttcg
    34981 cgaatttgcg gggtaacgtt cggttcgctg gacatatttg ataccaccga cgccagttca
    35041 gcatcacggt tgtgtgcatc gcggcgggca atacgatacg ccagacggtt atcacgcccc
    35101 tgatggtatt gctccagtat ggcatcgaga taccggcagt tggcctctgt ggcgcgttcg
    35161 agcattcgcg gcagattgcg aaactgccag tcaggccaga tgtagctcac tgccgcccac
    35221 gcaatggcac aaccaatcag cgtatcgatt acgcgaggta acgctacttc aaaaccttca
    35281 cccagcaagt taaaacacag tagcaccaaa agtgtgatga acatcgttgc atgggcatat
    35341 tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa ccagtagcac cagctgccct
    35401 tccagtgacg gtacaaacca cagcacagga atgccaatgg cgataccgac cagcgtacca
    35461 ataatcctta acttcaggcg gtggcgcgtg gcgttatagt ttggctggca gacaaacaaa
    35521 cttgtcagca agatccaata cccgtgatgc attccggtta tttgaatgat ggcgtagccg
    35581 aagcacaaca ccagcgacat tcttatcgca tgacggaaga gggcggattc cggcgtgaag
    35641 tgacggctaa gacgcagcca gatatcactc aacccgtgcg ggctgtcatc agcgagctca
    35701 ttttcgtcat tattatgggg tagtgcctgg gcctgttctg attcaattgt tgccagttgg
    35761 gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg ttttgagtaa atcggcgggt
    35821 gcgccgttat cgcgcatccg ctccagcgca gcatcaatat gcgtaaaagc gcgctcaaaa
    35881 tgcggatcat gttgataagg ctgacgcaac aaaatacagc gtgacagttg ctggcacgcc
    35941 tggccctgca tcgacatcag ccgctgaaaa cggaacagca cgtcgctgtg gcgaaaatgt
    36001 tcacgcaatg tttgatactg aatatgagaa gagctggcac gctcgtgaat atcctgtgcg
    36061 acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac cacgatcgcc acgtaagcgg
    36121 gtcagcagcg agagtttcgt ctgattcaat gtcgccatca gcagaccgtt ggcgagagcc
    36181 aaatcgtaca gcggtgcctg gctttcatct tcaatatcag gatcaaacat gcgcgacttg
    36241 agctcaagat aacgcgccag ttgttcatag caacgcgcca ggttgtcctg cagcgggcgg
    36301 accgggaaca gcagatgacc aataagtgtc aggacgttgt accagacggc accggccagc
    36361 agatacatcg gctgctgata ccagtgctca tacagtgatg ttcccaacat agtgtaaatg
    36421 gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt gacccagacc gccgagcaaa
    36481 atgaagccgc tggtagagag cgttaagcca atcgcaaata gccagggcca gggaaacagc
    36541 aattctactg aggccgaggc gataaaaaag cagaacagcg taatgatgag gttacgcaaa
    36601 cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg ccaccatccc cagcgttagc
    36661 ggaatcgtca gttttacatc acccagccac cacggaaacg ctgtggttcc acaaagcgca
    36721 ataaaaatac gcgcgtaata cagccaggcg ctgttccagg tatagcgttt gagcaaagga
    36781 cttagcataa aggccatatt agtggattat tcaaaacgac gacgtgcatt ggcttcacgc
    36841 gcagcttgcg ctgtttctac cgataccacg cgacgaccaa ccggccatag tgcaatagcg
    36901 gcaattttaa agttcgcaat gccgacagga atgccaatga ttgaaataca ttgtgcgatg
    36961 cccgttgcaa tgtgcatcag gcataaccac cagccaaaga aaatcagcca gaaaatattc
    37021 aataccgtac cgccagtatt cagcagcaca tttttgccag ccgggttcag ttcatcgaca
    37081 tgaatagctt cattgccata aggcaccaga gacagtttag tgatctccca gcaggatcgt
    37141 gtcagcggta aggtaaaaat cagcacaata ctgaccagag tcgccaacag ccagcccaga
    37201 gtggtggcaa atccgccaag cacaaagttc agaatgttca aaacggtacg cataaaacct
    37261 cgctttactg tggttttcag taatgggcgg caattgtaac gtttttttgg gctggagcac
    37321 gttttctctg acggttacac tgataagaaa taatttcgtg tggatctaca gagtcatgga
    37381 actgaaagcg acaacgcttg gaaaacgtct ggcacagcac ccttacgatc gggcggtgat
    37441 cctcaatgcc gggattaaag tctccggcga tcgccacgaa taccttattc ctttcaatca
    37501 attactggcg attcactgta agcgcggtct ggtatggggc gagctggaat ttgtactgcc
    37561 ggacgaaaaa gtggtgcgtc tgcacggcac cgaatggggc gagacgcagc gtttttacca
    37621 tcatcttgat gctcactggc ggcggtggag tggcgagatg agcgaaattg cgtctggtgt
    37681 tttacgccag caactggatt tgattgccac gcgcactgga gaaaataaat ggctgacgcg
    37741 tgagcaaacc tctggcgttc agcaacaaat ccgccaggct ttgtcggcgt tgccgttgcc
    37801 ggttaaccga ctggaagaat tcgataactg ccgtgaggcg tggcgtaaat gtcaggcctg
    37861 gttgaaagat attgaaagcg ctcggttgca gcataaccag gcgtataccg aagccatgct
    37921 taccgagtat gcggattttt tccgccaggt cgagtcttca ccgctgaatc cggcgcaggc
    37981 ccgggcagtc gttaatggcg agcattctct gttagtgctg gcaggtgcag gaagcggaaa
    38041 aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg cgtggtgaag cgtcccctga
    38101 gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa gagatggacg agcgtattcg
    38161 cgaacggcta cataccgaag acattaccgc acgcacgttt catgcgctgg cgctgcatat
    38221 tattcagcag ggcagcaaaa aagttccgat agtcagcaaa ctggaaaatg ataccgctgc
    38281 ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc agcgaaaaga aagcgcaggc
    38341 gaagggctgg cggcaatggc tgacggaaga aatgcagtgg tcagtgccag aaggtaactt
    38401 ctgggatgat gaaaaattac agcgtcgtct tgcctcacgc ctcgatcgtt gggtaagtct
    38461 gatgcggatg cacggtggtg cacaggcaga aatgattgcc agtgcacccg aagagattcg
    38521 cgatctgttc agtaaacgta tcaagttgat ggccccgtta ttaaaagcct ggaaaggtgc
    38581 gctgaaggca gaaaacgctg tcgatttttc gggccttatt catcaggcga ttgtgattct
    38641 ggagaaaggc cgctttatca gcccgtggaa gcatattctg gttgatgaat ttcaggatat
    38701 ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag caaaacagtc agacgacgtt
    38761 gttcgccgtt ggtgatgact ggcaggcgat ttaccgattc agcggtgcgc aaatgtcgct
    38821 caccaccgct ttccatgaaa actttggtga aggcgatcgc tgtgatttag acacgactta
    38881 ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt attcagcaga acccaggcca
    38941 gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa aaagccgtca cgttattgga
    39001 tgagagtcaa cttgacgctt tgctggataa gctctctggt tatgccaaac cggaagagcg
    39061 cattctgatc ctggcgcgtt accatcacat gaggcctgcc agcctggaaa aagcggcaac
    39121 acgctggccg aagttgcaaa tcgactttat gaccattcat gccagcaaag ggcaacaggc
    39181 ggattacgtc atcatcgttg gcttgcagga ggggagtgat ggttttccgg ctgcggcgcg
    39241 ggagtcgatt atggaagagg cgctactgcc accggttgag gatttcccgg acgctgaaga
    39301 acggcggtta atgtacgtgg cgctgacccg ggcacgccat cgggtatggg cactgtttaa
    39361 caaagagaat ccctctccct ttgtggaaat actgaaaaat ctggatgtgc cggtagcgag
    39421 aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat attacttcag acggtccgcg
    39481 agataacgct gataatcggg gatcagaata tcgaccgcgt cgttgaaatg cggcgactgg
    39541 attatgaagt ctgccgttgc cacgttggtg gcgaccggaa tgttccatac cgtcgccaga
    39601 cgcaacaagg ctttcacgtc aggatcgtgc ggcacggcat ttagtgggtc ccagaagaaa
    39661 atcaatacat caattttccc ttctgagatc aatgcgccaa cctgctggtc accccccatt
    39721 gggccactca acatcgcgtt gacgttcatg ccggtcgcgc gggaaattaa gttaccggta
    39781 gtgcctgttg catacagtac gtgttgttcc agtaacggtt gatgccgttc cacccagctc
    39841 atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa tatgtttccg cgcaggtaaa
    39901 gtgcgagtcg tcagttccat aatgtacatc cgtagttaac tttcctacag attactgtaa
    39961 gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc acaggcacaa aaatctcagg
    40021 aagatggttg tttttccgcc cattgtagga aagtatttcg cgtttgtggg tcagccagtt
    40081 taaaccaata cttcagccgt tgttctgtga gcacctgaga ctgcggtgga atagcatcca
    40141 gctcggaaac acccgaaagc agcgtactgt catctgccat catcgtggca aattgcggta
    40201 gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc gacctcataa tcaatcgttt
    40261 ttgccgttga ggtaatggcg agaatatcca gctttaccgg aatcggcgtc gcatcgccat
    40321 ccaacaattc aaggcgcggc gcggcatcaa aatggttagc ttcccgctca ttctccaggc
    40381 gaggcaggcg aaaattgacc tggttgatga gctgggtatt aaaactgacc accagcggtg
    40441 gggagatata cagccgttct tcactattgg aaagatgaat cgtcttctca acgcgaaaca
    40501 ctaactgatg cggtccatta tccagttcaa tgctgtccgc accgcgcagc agagagctgg
    40561 aaaccttctt gccgtcgagt accagaagat cgacatcggt tgaaagccgc agcgtggtgg
    40621 caaaaacgga taccggcaaa cataatgcaa tcaaggtggt cacgatgccg gttttcatag
    40681 gtagctcctg tcaaaagacc gcccgaataa taatgtgtca aaatttttct ttaatcatac
    40741 ttactaacat atagacatat ttcccgcttt tgctctcatt cattcgtatt agctgcatgg
    40801 ttggcatgtc ggctttgtcg tacactttgt taaacagtct ggaggaaata atgaaagaaa
    40861 ccgatattgc aggcatttta acgtccactc acaccattgc gctggtgggg gcaagcgaca
    40921 aacccgatcg tcccagctat cgcgtgatga aatatctact cgaccagggg tatcacgtca
    40981 ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg ccagcagggg tatggcacgc
    41041 tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt ccgtaattca gaagcggcgt
    41101 ggggcgtggc acaggaagct attgccattg gcgcgaaaac cctctggatg caactggggg
    41161 tgattaatga gcaagcggca gtactggcac gggatgccgg gttgaatgtg gtgatggatc
    41221 gctgcccggc tattgagatc cctcgcctgg gcctggccaa ataaaaaatc cccggaaggc
    41281 aaaaaccttc cggggattcg ttcagggatc agttacgcag acgcggggcc tggagttgtt
    41341 tgcggatggt ctgcgccagc tcatccatgg atggctgttc aggatgttca tcctgcaatt
    41401 cgctacttag ctgggcttca gccaggtagg tatgaaccgg taggccgtta tcgtcctcca
    41461 tcactacgtg ataccacgga gcggcgcgaa gctcgtcatt taccgccagc tcatcaggcg
    41521 acggttcaga gagcgaataa accgggtcga tatccacgac cactccgaga taacctaaca
    41581 gggaatggcg gacctgctgg ccgataccga atttgctggc aatcatagtc acctcccggg
    41641 aaatctttac ctttgatata agggtaaaat tccacatttc aagttacatg acgcgacagg
    41701 caaacccttt cagatatagc ccttccggat aggtagcgat caccggatga tcggctgcct
    41761 gacggaactg ctctataaat tgtacatcac ggccggcatc aattgcggca tccgcgatga
    41821 ttttctgaaa taaatcgctg gtcatcagac cggaacagga gaaagtcagg agaataccgc
    41881 cttcattcag caactgaatc gccagcatgt tgatatcttt atagccacga cacgcgccca
    41941 tcaactggct tttattctca acaaacttcg gcgggtccat cacgataacg tcaaattttt
    42001 caccgcgatc gcgataagta cgcagcaatt taaagacatc atcacggaca aactcagcct
    42061 tgctcagatc cagtttgttc agctcaacgt tctgccgtgc aatatccagc gcttcctggg
    42121 aggtatcaac gctgacaacc tggctgcaac cgcccatcag tgccgatacg gcgaaaccac
    42181 cggtatagga gaaacagttc agcacacgtt tattttcaac gtagcggcgg gtagccaggc
    42241 ggctatcacg ctggtccagg tagtagcccg ttttgtgtcc gtgctgaata tccaccagca
    42301 gtttcattcc gtgttcttca atcggcagca gggcaggtgg caactcgccg gtgacggggc
    42361 cctgggtcag ctccattcct tcttttttac gtaccgcgac gtcgctgcga tcgtaaatcg
    42421 aacattccgg gtacagcgtt tgcagggcac taattaatgc cgcgcgctga tattctgccc
    42481 cagcactcag cagttgcagc accagaaaat taccgaaacg atcgatagta atacccggca
    42541 ggccatcaga ttctccggcg attaaacgat agctgtcgag gccatctttt tgcgccagcc
    42601 agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa aaaagcaatg tcgatagact
    42661 cagacgggtc aaacgtccag acgcgcgccc ggatttgcga agctggcgaa taagcgccgc
    42721 gtgctaacca ttttccctga tgatcaacaa tatcgatggt ttcaccgagg ctggctttac
    42781 cttccatgcg ggcaacggcc ccggaaaaga cccacggatg gcgacgaagt aatgattttt
    42841 cgcgcccttt ggctaacact aaacgtacac tcataattta cttttctgtc gatgcaaaga
    42901 aatgggcgtc attgtccgga gttttccatg gatttgcaac gcgtccatta aggataagga
    42961 gagcagcaat gtcgaaagtc tgcataattg cctgggttta cgggcgagtt cagggcgtag
    43021 gatttcgcta caccacacag tacgaagcga aaagactggg tttaaccggg tacgccaaaa
    43081 atcttgacga tggcagcgtg gaagtggttg cttgcggtga agaagggcag gtggaaaaat
    43141 taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg tgtagaacgg gtgctcagcg
    43201 agccgcatca tccctcgggg gaattaaccg attttcgtat ccgttaaata cattttaccg
    43261 gttttggcag gccagcaatt ttggtggctt gcttagccgg acctttcgga aacagtcggt
    43321 ataaatagcg gctgttacct ttttcttcgc caaatttatt cgccatcgct tttaccagca
    43381 tacgaatcgc cggagaagta ttgaattcca gatagaaatc acgcacaaaa cgcaccactt
    43441 cccagtgttc cggtgacagc gaaatccctt cgttctctgc aatcaccaca gccagcggct
    43501 cactccactg gctgctttct ttgagatagc cttcggtatc cgtttctatc tctttacctt
    43561 cgaagatcag cataattatt actaccttaa tcagactggc ggcagtgtaa caaaaaacaa
    43621 agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg agattaatcg cggctagcga
    43681 agcccagaat gctcagcagg ctgacgaaga tgttgtacag cgaaacatac aggctaaccg
    43741 tggcacgaat atagttcgtt tcaccgccat gaatgatgtt gctggtttca aacaaaatag
    43801 cgccagagga gatcaaaatg aagaccgcgc tgatcgccag atgcagagca ggcagctgca
    43861 ggaagatatt cgcaaccata ccaatcagca ccaccacaat acccgccatc agcataccac
    43921 cgaggaacga catatctttg cgggtggtca gcacatatgc agagcagcag aagaacacta
    43981 acgccgttcc gcccagtgcc atagcgatta cgtcacccat tccggcagac agataggtgt
    44041 tcagaatagg tccgaggata taacccagaa aaccggtaaa ggcgaatgcg gagataatcc
    44101 cggtcggctt attcgccgtt ttataggtca ggaacatcaa accatacata cccaccagcg
    44161 tcagaatcag acccggagat ggcagcatca gcaccgtact ggcagttgcg gtaatcgccg
    44221 aaaaggccag cgtcaggctc agcagaaaat aggtattacg cagcacctta tgggtgctaa
    44281 gcagtgatgt acggtcatgt gaagaactaa caatacgatc cattgagtca ctctctatga
    44341 cagatgtaat taattaagca gcataatgat aatgcgtaag ggcacccaga agttttaccc
    44401 atctttacgc atttgatctg gaacaggttt aacagcggat tatcaggtca ttaagcaaat
    44461 ataacgccct gagaatttcg acaggcaaaa gaaaaagggg ttagcattta gctaaccccc
    44521 tatcttattt ggcggaagcg cagagattcg aactctggaa ccctttcggg tcgccggttt
    44581 tcaagaccgg tgccttcaac cgctcggcca cacttccgga atgacgcgca ctataaacat
    44641 cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct gaaaaacagc caaaagtgca
    44701 ttgatagcgt gaaataacag cagattgatc atttcatcac catgaattcc ttctctttta
    44761 ctcgtttagc aaccggctaa acatccccac cgcccggcca aaagaaaaat aggtccattt
    44821 ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt ttcactacgc
    44881 tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct ttcgttttca
    44941 gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc gaccataagc
    45001 gcccggtgtc ctgccggtgt cgcaaggaga agagacgtgc gatatgaata acgaggaaac
    45061 attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca aatattgtag
    45121 tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg cctgggcgct
    45181 ggagaacaaa ccccgcattc cggtggtatg gatccacggt ctggaatgca cctgctgtac
    45241 cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt ccctgatttc
    45301 cctcgattat gacgatactt tgatggctgc cgccggaacc caggcggaag aagtcttcga
    45361 agacatcatc acgcaataca atggcaaata tatcctcgca gtagaaggta atccgccgct
    45421 gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga aactcaaacg
    45481 tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct ggggctgcgt
    45541 gcaggccgcg cggcccaatc cgacgcaggc aacgcctatc gacaaagtca tcaccgacaa
    45601 acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg ccatcattac
    45661 ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggccgtc cgctgatgtt
    45721 ctatggtcag cgaatccacg ataaatgcta tcgccgcgcc cacttcgacg ccggagagtt
    45781 cgtccagagt tgggatgatg acgctgcccg caaaggttac tgcctgtaca aaatgggctg
    45841 caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg gcgtttcttt
    45901 cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct gggatcgcgg
    45961 ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca ccgccgatac
    46021 cgtcggttta accgcgcttg gcgtggtggc agcggctgtt ggtgtgcacg cagtcgccag
    46081 cgccgttgac cagcgcagac gtcataacca gcaacctaca gaaaccgaac atcagccagg
    46141 caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac catcaataat
    46201 gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat gcgctgcgaa
    46261 gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac catgtttcgc
    46321 gggctggaga tcatcctaca agggcgcgac ccgcgcgatg cgtgggcgtt cgttgaacgt
    46381 atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat cgaagatgct
    46441 atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct ggcaacgctc
    46501 tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg gatcgatgtg
    46561 ttagatgcgc tgaaagccga cccgcggaaa acctccgaac tggcgcaaag tctctcctct
    46621 tggccgaaat catcccctgg ctatttcttc gacgtacaaa accgcctgaa aaaatttgtt
    46681 gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca gtacaaactg
    46741 ccgccagaag ctaacctgat gggctttgcc cactatctcg aagctctcga tttccagcgt
    46801 gaaattgtca aaatccacgc ggtctttggc ggtaaaaacc cgcatccaaa ctggattgtc
    46861 ggcgggatgc cttgcgccat caacattgac gaaagcggcg cggtcggggc agtcaatatg
    46921 gaacgcctga acctggtgca gtcaattatc acccgcacgg cggacttcat taacaacgtg
    46981 atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga aatcggcact
    47041 ggtctttctg ataaatgcgt tctcagctac ggcgcattcc cggatattgc caacgacttt
    47101 ggcgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt caacaatgtg
    47161 ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgacca cgcctggtat
    47221 cgatatccca acgatcaggt cgggcgtcat ccgttcgatg gcatcaccga cccgtggtac
    47281 aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga acaggaacgc
    47341 tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg gccgctggcg
    47401 cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt cgatcgcatg
    47461 atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttaggccg cattttgtgc
    47521 cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga caagctgatg
    47581 accaacctga aaaacggcaa tctcgccact gcttccacgg aaaaatggga acctgcaacc
    47641 tggccgacag agtgccgtgg tgtcggtttt accgaagcgc cgcgcggggc gttaggccac
    47701 tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc gaccacctgg
    47761 aacgccagcc cgcgcgatcc caaagggcag attggcgctt atgaagcggc gctgatgaac
    47821 accaaaatgg cgatccccga gcaaccgctg gagatcctgc gtactctgca cagctttgac
    47881 ccgtgcctcg cctgttcaac acacgtgctg ggcgacgacg gtagcgagct gatctccgtg
    47941 caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa cgttgtcagc
    48001 cactatgtct ttgaagcgcc agtccgcatc tggcactggt tgacggtgtt atgcatggcg
    48061 gtgttgatgg tcaccggata ctttatcggc aagccgctac cttccgtcag cggcgaggcg
    48121 acgtatctgt tctatatggg ctacatcagg ttaattcact tcagcgccgg gatggttttt
    48181 accgtggttt tgctgatgcg gatctactgg gcttttgttg gcaatcgata ctcccgcgag
    48241 ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta tgaaatccgc
    48301 tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataatcc catcgcccag
    48361 gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcactgg ttttgcgctg
    48421 tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt ggaatttttc
    48481 tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg gatgtggctg
    48541 attggcgcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat catgtccgac
    48601 gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg caaaataagt
    48661 aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc aacctgctgt
    48721 gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat taccactggc
    48781 ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg gggtatgtcg
    48841 aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa cctggaacgc
    48901 tgcgaaccta tgccggagaa cgcattccgg cttatctcag cgcgaagaaa atgagcctgc
    48961 atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat ctgccagcac
    49021 atattgccct cgtcggtctg caacccgcta tgctcgacga ctacggcggc agcctgagcg
    49081 aactggcacg ggatcaactg cccgctgcgg aacaatcggc gttggcgcaa ctggctgcgt
    49141 ggggaattgt gccgcaaccg gctaatgaat cgcgctgtct caattatgac tgtctgtcga
    49201 tggaaaatta cgaaggcgtt cgtttgcgcc agtacaggat gacactggag gagcagggat
    49261 gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg gctggacgcc
    49321 ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg tggtgttatt
    49381 aagcagtgac ccgaaacgta cgccagaggt tagcgataat ccggtaatga ttggcgaatt
    49441 actgcgcgag tttcccgact atacatggca ggtggcgatt gctgaccttg agcagagcga
    49501 agccatcggc gatcgctttg gcgtctttcg ctttcccgcc acattagtgt ttaccggcgg
    49561 aaactatcgc ggtgtgctga atggtattca cccgtgggcg gaactgataa acctgatgcg
    49621 cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt ccatctgctg
    49681 gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc gatcacctgt
    49741 caggtgaatg atgaaccgag tatggcggcc ctggaacaat gtgctcacag cccgcaggtg
    49801 attgcgctgt taaacgagtt acaacatcaa atgagtcaac gccagccgcc gttgggcgag
    49861 gtgctggcag tcgatctgtt aaatctccac gccgacgatc gtcactttat caataccctt
    49921 ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtga aagtgaaata
    49981 caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga aaagttgctg
    50041 gaggacaaac tggaagctgg ctgtgcgccg ctggcgttgt ggcaggcggc aacgcaaaat
    50101 ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa tggcctaccg
    50161 ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca cagcattaat
    50221 ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcacgtct ctgtgggccg
    50281 ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc cacggggtta
    50341 cgccatgtct ggcatttacg ctgtacggac accttaaaag gccctttact ggaaagttat
    50401 gaaatctgcc cgataccgga agtggtgctg gcagcgccag aagatttggt cgactctgcg
    50461 cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta aaaagacggt
    50521 aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt cgtagagttt
    50581 caggataaag agggagatct accattatcg ggttattttt ctctcttcgc ctacaggagt
    50641 gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg cgctgtatca
    50701 ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca tggaaaccat
    50761 ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg gtaagctctt
    50821 cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc agtttggtac
    50881 taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac tggcgatgga
    50941 agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct tcggctggca
    51001 acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg gttcaaatct
    51061 ctctgcgttg tggatattga atgccaacgg ttggatgcaa tacccgaccg gtgcgcattt
    51121 tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct ttaacccggt
    51181 cagccaggtg aaatttgtgc acaccgtaat ggcgggctac gtgaccgggg ccatgtttat
    51241 tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg cattacgttc
    51301 gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc aactcggaga
    51361 cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga tggaagggga
    51421 gtggcaaacg gaacctgcac ctgcaccgtt ccatgtggtt gcctggccgg aacaggatca
    51481 agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg ccactcactc
    51541 attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc cacgcttgca
    51601 acgcggacgt atggcctggc tgttaatgca ggaaatatcg caaggcaatc gtgagccgca
    51661 tgtgttgcag gcattccggg aactggaagg tgacctgggc tacggcatgt tgctctcccg
    51721 ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga tgcgtggcgc
    51781 gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct gtggttccct
    51841 gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa tcgaccagca
    51901 tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg cgattgaagc
    51961 cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca tcttaccgac
    52021 atactccgcg cactccgctt taaccacagg acaactggct ttctcactga tcatgatcgt
    52081 agggctttac accctgttct taatcgccga agtctacctg atgcagaaat atgcccgtct
    52141 ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa ggagaaaatc
    52201 atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt gatcctggtg
    52261 gtctttatga tctccgacgg atttgacatg gggatcggct gtctgctgcc gctggtggcg
    52321 cgtaatgatg atgaacgccg gatagtgata aacagcgttg gtgcacactg ggaaggcaac
    52381 caggtctggt tgatcctcgc tggtggggca ttatttgccg cctggcccag agtgtatgca
    52441 gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct gttcttccgt
    52501 ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa aatgtgggat
    52561 gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc cttcggcaac
    52621 ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta tctcggcagc
    52681 ttctggcaac tgctgacgcc attcccttta ttgtgcggat tgctcagcct tgggatggtg
    52741 attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca tctgcgttca
    52801 cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct ggcgggttac
    52861 tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa cggtccttcc
    52921 aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa tttcgtcgaa
    52981 tcgcccgttt tgtggatctt cccgctgctg gggttctttt gcccattgct gacggtgatg
    53041 gcgatttatc gtggtcgccc ggggtgggga tttttgatgg cgtcattgat gcaatttggc
    53101 gtgattttca cggcaggcat cacgctgttc cccttcgtca tgccgtcaag cgtgagtccg
    53161 atctccagcc tgacgttgtg ggacagcact tccagccagc tgacgctgag cattatgttg
    53221 gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag ctactacaaa
    53281 atgtgggggc gcatgacaac agaaactctc cgccgtaacg aaaacgagtt gtactaagga
    53341 gcaggaacaa tgtggtattt actttggttc gtcggcattt tgttgatgtg ttcgctctcc
    53401 acccttgtgt tggtatggct ggacccgcgt ctgaaaagtt aacgaacgta ggcctgatgc
    53461 ggcgcattag catcgcatca ggcaatcaat aatgtcagat atgaaaagcg gaaacatatc
    53521 gatgaaagcg atcttaatcc catttttatc tcttctgatt ccgttaaccc cgcaatctgc
    53581 attcgctcag agtgagccgg agctgaagct ggaaagtgtg gtgattgtca gtcgtcatgg
    53641 tgtgcgtgct ccaaccaagg ccacgcaact gatgcaggat gtcaccccag acgcatggcc
    53701 aacctggccg gtaaaactgg gttggctgac accgcgcggt ggtgagctaa tcgcctatct
    53761 cggacattac caacgccagc gtctggtagc cgacggattg ctggcgaaaa agggctgccc
    53821 gcagtctggt caggtcgcga ttattgctga tgtcgacgag cgtacccgta aaacaggcga
    53881 agccttcgcc gccgggctgg cacctgactg tgcaataacc gtacataccc aggcagatac
    53941 gtccagtccc gatccgttat ttaatcctct aaaaactggc gtttgccaac tggataatgc
    54001 gaacgtgact gacgcgatcc tcagcagggc aggagggtca attgctgact ttaccgggca
    54061 tcggcaaacg gcgtttcgcg aactggaacg ggtgcttaat tttccgcaat caaacttgtg
    54121 ccttaaacgt gagaaacagg acgaaagctg ttcattaacg caggcattac catcggaact
    54181 caaggtgagc gccgacaatg tctcattaac cggtgcggta agcctcgcat caatgctgac
    54241 ggagatattt ctcctgcaac aagcacaggg aatgccggag ccggggtggg gaaggatcac
    54301 cgattcacgc cagtggaaca ccttgctaag tttgcataac gcgcaatttt atttgctaca
    54361 acgcacgcca gaggttgccc gcagccgcgc caccccgtta ttggatttga tcatggcagc
    54421 gttgacgccc catccaccgc aaaaacaggc gtatggtgtg acattaccca cttcagtact
    54481 gtttattgcc ggacacgata ctaatctggc aaatctcggc ggcgcactgg agctcaactg
    54541 gacgcttccc ggtcagccgg ataacacgcc gccaggtggt gaactggtgt ttgaacgctg
    54601 gcgtcggcta agcgataaca gccagtggat tcaggtttcg ctggtcttcc agactttaca
    54661 gcagatgcgt gataaaacgc cgctgtcatt aaatacgccg cccggagagg tgaaactgac
    54721 cctggcagga tgtgaagagc gaaatgcgca gggcatgtgt tcgttggcag gttttacgca
    54781 aatcgtgaat gaagcacgca taccggcgtg cagtttgtaa tgcataaaaa agagcattca
    54841 gttacctgaa tgctctgagg ctgatgacaa acgaagaact gtctaatgcg tagaccggaa
    54901 aaggcgttca cgccgcatcc ggccactttc agttttactc tttctcggag taactataac
    54961 cgtaatagtt atagccgtaa ctgtaagcgg tgctggcgcg tttaatcaca ccattgagga
    55021 tagcgccttt aatattgacg cctgcctgtt ccagacgctg cattgacaaa ctcacctctt
    55081 tggcggtgtt caagccaaaa cgcgcaacca gcaggctggt gccaacagaa cgccccacga
    55141 ccgcggcatc actcaccgcc agcatcggcg gcgtatcgac aatcaccaga tcgtaatggt
    55201 cgttcgccca ttccagtaat tgacgcatcc gatcgcgcat cagcagttca gacgggttag
    55261 gtggcacctg accgcgagta atcacatcaa agcctccttt gccaaaatgc tggatcactt
    55321 tgttgagctc atctttacct gccagatatt ccgacaagcc atgttcatta ctcacggtaa
    55381 acaggttatg cgaataacca cggcgtaagt cggcatcaat aaataacact ttttgatcgg
    55441 actgggcgat caccgctgcc agagttgaac tgacaaacgt tttaccactg tctggcgtcg
    55501 caccggtgat catcagaatg ttattctccg tctccatcat agcgaaatgc agactggttc
    55561 gtagcgcacg tacggcttcc acagcagaat ccgccgggtt atccaccgcc aggaagggga
    55621 tatttttagt acgatggcgc tgctgattag aaaataaatt tttcttacgc agacgggtgc
    55681 gtttatccag ccactcggac attgggatag tggcataaac gctgatgccg tgctcttcca
    55741 gttgttccgg ggcttctaca ccacgacgca acatcgcacg cgccagcacg gcacccacag
    55801 aaataaacag gccaagaata aaaccaagca ccacattcaa cgcttttttc ggtttcactg
    55861 gctgcggctg agtgactgcc gggtcgataa tccgcacgtt accaatggca ctggatttcg
    55921 aaatactcaa ctcctgctgg cggttaagta attgcagata taccgcacgg cccgcttcta
    55981 cgtcacgact taaacgcaac acttcctgtt gggtggaagg cattgccgat acccgcttat
    56041 tcaggcgttt gcgttcttgc tccagcgtct ggcgtttttc cagcagcgca cgataagttg
    56101 ggtgatcttt cttatacagc tgggagatct ctgcctcgcg gaaggtcagc tcattgagtt
    56161 gattatcaac gttcacaatc tgctcaagaa cggctttggc ttccaggtta aggtcaaccg
    56221 aatcgcgctg ctggcgataa acgttgagtt tttcttccgc ctggtccagc tcgctgcgca
    56281 cttcaggtaa ctggcgctgt aagaattcaa ggctttgtga atcctgcgcc gcctggcgag
    56341 cgatattctg ttgcaaatag ttgttagcga tgctgttcag aatacgagta atcaactggg
    56401 gatcatcacc agtcatggta agttccagca tcccgctttc tttactgcgt tcgctaacgg
    56461 taaaggtttc ctgcaatgcg ttaatcgctt ccagttcggt acgctggctc aggacaaact
    56521 gtgttcctgg tttggcctta atgtccgcga tagtcagcgc aacgccatct ttttccagac
    56581 gctggccgac cataccattg acggtgaact cttcaccttc cagtgtatag tggccgtttt
    56641 ccccaaccgt gagtgtcagt tgctgatcct gaccattcag ttgtggaata tgcatccagc
    56701 tgatcgccag ctcacctggt ttttctttgg ttaatctcgc ccagccgcga cccacaatcg
    56761 gaaaatactt ctgctcaact atgtcgcgca gattcagttc agcaatggtt ttaccgagaa
    56821 tcatgcgcga ttgcagcagt tggatctccg gtgcagactc gggcgatgag ttagggatca
    56881 tatcgctcag gccgctgaga atggcgttgc cctgtttttg ctcaacctgg accagagtat
    56941 ctgcctgata aattggtgta cttaacagcg agtaagcgac agcgatcagc gtgaataacg
    57001 cggtcacgct gataataaac ttacggtgat cccataactc gccgaccaga cgaagcagat
    57061 cgatctcatt ttcctgagtg ctgcctggtg gcgtattcat atttttagtt gtcatcgtat
    57121 tttataaact caaattaccg gctgaggcgc ttcgcccatt cctgactggc gcgctccaac
    57181 ataccgtaga catgttcaaa tgcgtcctga cttttacgat agggatccgg gatctctttc
    57241 tgttccagcc attgcccaaa cagcattgtc ttcccgcgga cctcgggagc aattgcggta
    57301 acctgagcaa tatgttccga ctccattgcc agaatcagat cgtaatttcg tgccatctca
    57361 gcagtgagct tacgtccggc atgtccttca agagatacgc catgattagc ggcaacatct
    57421 gccgctgtcg catctgcagg gtgttttacc agaccatgaa cacctgccga tttgactttt
    57481 acgcccggca gacgtttacg cagcaagcgc tcgccaatgg gcgaacggca aatgttgccg
    57541 gtacaaacca ccaggattga gttaaatttt agttgggcca tgtttgaatc caccgtgaag
    57601 tctctgtcag gtcatgcacc ccggaaatcg tcggtaccag cagagaaatc acacggttcc
    57661 aacgagccag cggcgcggta gtgacataaa caatgtcgta aggttcaagc tggaattccg
    57721 ttccgagtat cattgccgat gcgtccttcg cattcagctg ataaatattg gcgattttgc
    57781 cattctgctt gttttgcgtt gcacgaataa caaaaatccc cgtcgcatca gcgacatcct
    57841 gattcatacc ctcagcgtta cccagcgctt ccgccagggt cataccgctg cggtccattt
    57901 tcagcgtgct ctgtttaccc acttcaccca tcacgaagac tttcagatca tcgttgcgcg
    57961 gaataaataa aatatcgccc gggtgcagca gtttgttctg gcgcaagtcg ccacgctgca
    58021 tcagcgcata gaggttcact ttggtcttta cgccattctg ggtgagcacc acgttgcgcc
    58081 agtctgcatc tgcggtcaga ccgcccgcgg cgttaatcgc atccatgatg gtcagaggaa
    58141 tattggtaat cggctgctga cccgatttgc tgacttcgcc ggtgacgtac gctttttgcg
    58201 agcggaacgc cgcgacgctg acatcgactt gcgggctttc aatgactgaa tccagacgcg
    58261 ctgtaatctc gttgcgtacc tgggtaagtg ttttcccggc cactttcagg cgaccaatat
    58321 aggggtagaa aatcgcacca tcggcgttaa cccagttgcc ggtatcactg gcgctacggt
    58381 attgccctgc gggggtggtt agttccgggt gatcccagac cgtaaccatc aacacatccc
    58441 caatgccgat acgatattca tagtttgcga tttgctgctc cagctccggg ttagcctgcg
    58501 acataatcgg tttggcgcgc aactgatcaa tcaatcctgg cgtgacggga tagatattga
    58561 ccattttgtc caggtcatac tgattgtcag gtagttcaat gacatccttg ttactggtcg
    58621 ataaattctg accaggaaca agggtacatg ctgtcaaaga cagaacagcc aatgtcagta
    58681 ccgaaaactt aaaaatgttc tttttcattg gtttatattt atcagtcaat gtaatgttat
    58741 gaattacgtc aggttattgt ccaaccggta cttctcgctc tgccgtcatt ggatagagct
    58801 ggtatttgcg tgacagcatt tgtccaccat cacgcgtcaa tggtgtccac gaaaccaccg
    58861 cgcggttgcg gttaggtcca atggtcatca agtcgaatgg aatcgagata taaaaacctt
    58921 tactaaagcc gccttcgccg tagtcatctt tagatacgtt actgattgcc gcccataccc
    58981 ctaccgccac gccgctgtca aagcgtttgg cgacgtcgat cgttgcgcct ttatcttttg
    59041 ccagatattg cccaacgcta agtttcatca gtacgccatt gagcgtcggc gggttccagt
    59101 aagccgtcac gaaacctgtt ggcgtggaat aatcggtgaa acgcatcatg ttgtcccagt
    59161 cacgttgctt cacgtagtta acgtccacac ccagcgccca gctggcatct agcgggcgat
    59221 aaagcagctc ggaaccgaca ccggcgtaca tcgtttccag ataaccgcca tacacctggc
    59281 catagaaacc attgcctaag tcggcaaagt agttcgcctg caagttgttg agataaacgt
    59341 cattgcgaac gtaatcacgg atatgcgtgc gcacgcgggg cagggtagag tccgcgggca
    59401 acagcgaaga cttaaacttg tcgtagttgt tgtaaatatt ggtgaaaata ccgccatcaa
    59461 gcagcaggtg gtcggtaaac cagtagcggg cactggacat cagccccagc tggaacatat
    59521 agaaatcttc cgggccgccc agcgactgtg aaagtgttgg gttgaaagag tagctaaagc
    59581 gatcttcacg aatacggtag ccccgaccaa aggcagaaag atcttctgct tcaacacgtt
    59641 gttgttgaag ttgctctgat tgaccaggcg ctgtaccagc cagctgtttg cgcaggctgg
    59701 ctacgtcggt ttcggtagtc accatcgcca tatgctcgcg cttttgcgtc acgctaatct
    59761 tctcaacgcc ttgcggcagg ttattcacca gaatccggtt ggcacgatcg accgcttcgc
    59821 gagaatcacg gtatttgtat tgttgaccag acatatacag cgtcttatcg cgcagctgaa
    59881 tttccggtgc ttcaaaacct gcgttgtact tcagcgcggt aagttggttc gctactgtgg
    59941 tgtactgcaa tccttcagat tcaggcgcag gttgatatgc cggttttggc gtatcgcgca
    60001 gggcagggcg cagatcgttg aaattggtcc gtaacgtgaa gccaaacatc aacgtgttac
    60061 cgcgttcata actcaggttg agatctgccc agctggcagc gcgataaact gcgccgacgt
    60121 tgaaatggct tgcctgaggc agtttgccag cgaaatcatt ctggtaattg ttgccatcgt
    60181 attcgagttt cagacgcagg ggattccacg gcgtttgata ctcaatgccg ccaaagatgg
    60241 aagccgggcc acgaaagata tcgctaaagc tgatatcgcc cgcatcgtga gactctgcgc
    60301 gatgacaata tttatcgctc acccggcaaa acgggttggt aatattgccc gcattaccgg
    60361 cgtagcccca tgccatcccg agggtgaaat caaatggccc cgcttgcttg ctggccacca
    60421 gatactcacc gtcaaacaga cccgtaccag caatatcacg tttaccaaac gccacttgcg
    60481 gtagccagta accttcttcc cacaggcgaa gtttaaaatc gaatgatttg tctttgtatg
    60541 actgatcgcc gctgaaatct tcccactggc tatatttgcg tgtgcgcaca tccgtataac
    60601 gaatggtgcc ttccagccat gggaacagcg ccacggaggt ggagtagaac cggtattgat
    60661 cgttatcccg gtagttgacg ctgaattcac cttccggtgc gatgcgcgca tttggcatct
    60721 gcaacaatcc tgtgccgcca aagtctgatt gcgacgaacc cagcggatcc gggtaggtta
    60781 atacttcagc atgacaggca ctggagacgg caatggccag gcagcttaaa agataagaat
    60841 tcttcttcat cagtcaggaa cacgttgcgt taaaacagac acaatctggt catttaaatc
    60901 agcatatttt tcgggcagta catgcgcaga aaagcccaac caaagctggc tgccaggcgg
    60961 tggttccaca tgtcttttat tccacagggc aacaggggcg acgacagtct caccctcagg
    61021 cgtaatcacc ataacgttat tcttgtccgc gcctgcaagg cgggggtgat cttgcagata
    61081 atcggtaacg ctacggcctg cctgccaggg caattgcccg gccccagaaa ccgcacccag
    61141 cagagtaata gtcaccggac gctgtacggt atagagcgta tagtccccga ccaacggggg
    61201 attgctgttc tcatccactc gtacaaaatc agggtccagt ttgaccggta agcgaccagt
    61261 gatattgaga ttgagtaact gctggcgcac ggatttaatc gttgccgcca catcgtcgtc
    61321 agcttctgct tcccaggaag caagctgtgc catgacatgt tggtagtctt taagcgcttt
    61381 agctttcgcc gcagagtcgg tcagcaaggc accaggccac cagaggcgat cgcgcagttg
    61441 cggttgtgtc accaactgga caacattttc catggggcca acggaaagtg tctgttgttc
    61501 gccaggcaga taaatagtca ccgttccttg cgcaaaggca tggggtgtca ttacgtaaag
    61561 tacgctggca atgaaatacg actgtaattt attcatggtg cgggtttaag aaacgtcatt
    61621 tctacgggaa taacccctgc gcctaacatt tgccgacttt gacgaacctg gccagtagcc
    61681 gaatcgaccc agaatgtgtt ttgccactgt ttttctggcc gcgtggaagt cacctcttcc
    61741 tgccagacat tacacagcac attgcgaccc gcaatattca gcgtctctaa tccagcaaag
    61801 gaaaaagtag aactcagggt cgcagagcga aaatggttgt cttcactcca gagaatatcg
    61861 cgcgtccagc ggctgccatc ttttattgcc agcgcgttac gcagagggtc ctgcccggaa
    61921 tttgtcactt ccagcagatt attattaagt ttgacggttt ttaaaagacg tccattgtgg
    61981 gtaaccagca tggcgttatc ctgggataac catttgcttt gttcttgttc tatatatccc
    62041 agtaccacaa agattcgttg cccttcatta aggcgtaaat acatagtgga ataaggcaac
    62101 gcctggatct gctgatccgc tacggtgata tcctgattat cgaaaaggct ggcgcgaaat
    62161 gtatcgacca tactttgctg gctgtgcgta catcccgcaa gaaatagtgc gaaaatcgat
    62221 aaaataagag ggcgcacctt taatccttga aaggttaatt aaaaaaatgc aaatctctaa
    62281 agcccggtac gaatatcgca accgggtatc atttaacttt aatttcgacg cgttaaagca
    62341 gtattagcgt gtagtggacg tggtggttga ggtgttatga gaaccgccat cgccactgtc
    62401 atttgctgca acaactgcca acgtcgccac accaacagcc gttgccacac cgacaactgt
    62461 cgccgtgccg acagcgccag cagccacgcc accagtggta gtagcagtcg tggttgcgcc
    62521 ggtagtggtc gttgtggtgc cagtagtacc cgtcgttgcc tcggtcccgt ttgttgcttc
    62581 aggggcagca aacgcagctg gcgtcgtcaa catgaaggcc ataaggattg cagaaagctt
    62641 gtgtttcata acttttcctt tattcatcgc atggacaata cgtacgcttg agatcaggat
    62701 tgtctacttt gagtataaca tccttgtgaa atctcccttt cctcataccg cttgcactat
    62761 atactgcggg taggaaaggc gcgcagaggg aaatataaga ttgttaacta tccccctctg
    62821 gaaatacgac ttgtgagcat aagataaatc ttagcaaaat acaaattaaa gataaggaaa
    62881 ttgttacgaa agctattaat tattgttggt aatatagttt caagtggtac tataactggc
    62941 tgttaaatgg catttataac tattaggtgc agagatattc gcttaaatgg attagtttct
    63001 ttgtggaaaa gtaactgatg ttataatgta tgatgttgac tattgcacta ttccaataaa
    63061 accagtcagc tttaaacaag cagcgtcata ttaagagaga taaacatttg ccgctgttgg
    63121 tcctcgcagg ccatttacgc ggcaaaattc cacacgtaat cctggtataa gcacttctgc
    63181 gtcgcgggga gtgaatgcgg aaatatggac ctgaacttct ttacgaccgt cggaggggat
    63241 aatgaatcct ttgccgcttt tgcgatcaaa ggttttgaca attcctgtca ttttacggga
    63301 caaacaaatt ccttactgaa aatactgcgc tgcactatac ggggttaata aaataaagcc
    63361 agcgatattt aagaccgccg gacggctaaa ataaaatttg cttaatctca attatcatgc
    63421 gttaatagct gcgtcggttt gaaagacaga cagcatacaa agtagtttac taaagcagtt
    63481 ctcattatca ggcattatcc ccttcttttg agtctctctc ctgaacacta agtagtttct
    63541 gtattaaagc cctgtttgcc gaaaggccca aaatgaagga agtaaaatat gtctaataaa
    63601 atgactggtt tagtaaaatg gtttaacgca gataaaggtt ttggctttat cactcctgat
    63661 gatggcagca aagacgtttt cgtccatttc accgccatcc agagcaatga attccgcacg
    63721 ctgaacgaaa atcagaaagt tgaattttct attgagcagg ggcaacgtgg ccccgcggca
    63781 gcgaacgttg ttacgctcta aggttgccgt tattactcaa catctccatt tccgctgtcc
    63841 atgttgtcat ggttcacagt accgcacatc ggcattcgat gtgacggagc gaaacccttt
    63901 gggcgctaag tgtatttttt gtaaatcgac gatgatcacc tttgataacg tcgcgctgca
    63961 aatacgcact gaccatgcgc cgctggattt cacaaaataa tattaggctc cctcgtggag
    64021 ccttttttat atctgcctta tttttcttca acgctgtatg tatagtaagc gataacctgt
    64081 tgattattga atctttcggg gagatggctt ataacatttc ttacctgacc agggtaccgg
    64141 gaaccaacac cttactggcg tgttgctgtc ttttaagacc agaagaggtt aacagtgaat
    64201 attgaagagt taaaaaaaca agccgaaacg gaaatcgccg actttatcgc gcaaaaaatc
    64261 gccgagctga acaagaatac agggaaagaa gtctctgaaa ttcgcttcac cgcacgagaa
    64321 aaaatgaccg ggcttgaaag ttatgatgtc aaaatcaaaa taatgtgatt ttgtgaacat
    64381 cacccgtgcg aggtgatgtt ccgcttgttg ctaatttagt gaccaatcat tggcgcttgt
    64441 ggaattaagc gtcggtacaa ttcatccggt accgggcttt gccatactcc cgcatacatt
    64501 gcgtaaccaa tcaccgcaaa cataatcccc agaaccagta gcgtcattaa ccagccagac
    64561 aacgcaaagg cttttttatt tgccgcaggt ttttgcagtg aaaaggtcaa tgttgaggct
    64621 accggacatg actctacgca agtcatacag ccggtacatt ccactgttcg tacctgaatt
    64681 aatttatcga ccgggatccg tgatgggcaa ttttttgcgc atttgccaca gtcgatacaa
    64741 ctttcggcat tgcgacgaat cttaaacggc gacaatagcg aaaccacgcc catcagcgcg
    64801 ccatatgggc aaagataacg acaccaggca tggcgaataa acaggctggc aatcagcaaa
    64861 acggtcacgc tgattaatgt cgcggtcccc atatgacgaa agaaatcgag cattttaacg
    64921 tccatcacca cgctgtaggg cgacaacata aaatagtgaa tcgcctgagc gggcatcaat
    64981 aatgcgatat agagaaaaaa actcaacagc aaatacttca cgccgcgcag aggaatatcc
    65041 agccagcggg gaaggacaca ttgccgacca aacagtttgt taccgagatc gccgattaat
    65101 tcagaaagcg taccaaccgg gcataaccat gagcaaaagg cctttttgag taatagactg
    65161 atgacgataa aagcgaccaa taacagcatc gcggcggcgt ggacggacgg taactgacct
    65221 gttgtaaggc tatatttcag attcatcaga ccggcaatcg gtagccagcc ttcgatacct
    65281 cccggtctgg cgacaaatgt cgtgctactt gccgtttcgt aatagcgcac ccaataccag
    65341 aacgtgatgg caatataaat attcattgcc aacagtaata attgcgtcgc tttacgccag
    65401 gtcgtggcat tgcgccagtc attccacggt aatttgccgc ccgtcgtgcc tggccgccgc
    65461 tgccagcggg ttcttttttt ctctgccatg attttgccag tccgttaagt tgcataccaa
    65521 atgccactat tctagttgtt cttaactggc tgatattgat tcaaattgcg ttcaggtctt
    65581 tcttattcaa ccatgcttcc agatcggcaa cactgcgtgt aatttcttcg tgtggaaggg
    65641 gggcagataa tggctgctgc tccagttgtg cgcatagctg gctggcgata cgcattccca
    65701 aattcgagca actgcttttt agctgatgcg cggcacgctt tattttctcg ctatcctgac
    65761 tggcgcgggc aatgtcgatt tcatcgagaa gcggcagggc atgttgtgta aataatacca
    65821 gccattcgtg gatcttctcc gtccccatta actgagcatc ttcattgagt tgcgatacat
    65881 ccagcgattg atcgttattg acttgcagtt ggaaatagtg cgccagtaac tgaccgagca
    65941 cttcacgcgg caccggttta gggataatcc cgcggaataa tgaactggta cgctggcgca
    66001 gcgtttcgtc aatgacatgg gcgctaaagc caatcaaaac cagcgacgga tattgctgtg
    66061 ccagttgtcg ggcaagcgta atgccgtcga catccggcag atcaaaatcc accagtgcgg
    66121 cagcaaacgg ttcgctattt tgcagtgtct ctaaagcctg cgcggcattg ccaacagcaa
    66181 caacctgcgc accactggtg ttcagcatct caacggtaat tcgctgggtt agcgggttat
    66241 cttcaattaa cagcaaacgt aaaccgtcaa gacgcaccgc ctgattgact gtttttggca
    66301 cgggtgccgt ggcaacacgt aacggcaagc gtaaacaaaa acagcttcca acctccggcg
    66361 tgctggtggc gctcagttcg ccgcccatcg cctgggccag acggctactg atagtcagcc
    66421 ccagcccggt gccgccgcgt ttgccgctta cctgcacaaa tggctggaag atttctgcca
    66481 gtttcgcggg atcaataccg cagccgctgt cttccacttc gaccagccat tgctcgccat
    66541 cagtgcgact acgcaggata atgtaccctt cgtcagtaaa acgcagggcg ttgctcaaca
    66601 ggttggttat aacctgacga atacgtcgtg gatcgcccat taacgcgcac ggcatatcat
    66661 cggcaattgc cgttgccagg cgaatcgggc gacctttcac ccgtccgctc attaattgca
    66721 gggtactttc cagcagcggg cgcggttcaa agggctcatc gctgaccgaa acattcttgc
    66781 cacctgcttc gatagcggaa taatcgagaa tatcgttgag gatggtcagc aacgattcac
    66841 cagagtcagt aattgcccgc aaatcatcac gctgggcgtt aagtgcgggg ttatctgcca
    66901 gcagttgagc agtgccgaga ataccgtaca gcggtgtgcg gatctcatgg ctcatcgccg
    66961 ccagaaacgc cgattttgcc tggctggctt tttctgcttc cgcccgtgcc tgtcggtgtt
    67021 ctatcaccag ttcctgcaat tcagctgtac gcgctttgac ctgcgccgcc agctgttcgc
    67081 ggtggcgatt cagtgcatga acattgctgc gaaacgcatc catcagccgc ccgatggtat
    67141 ccagctcccg tacgccagcg gtttccggga aaggggagtc aatatcaccg tccagcagcc
    67201 gttgcagcgc ctgcgtttgt tcggcaagtg gacgcgtgac tgagcgataa accacgcgcc
    67261 aaaggatcag aatcagtgcg caaagtgaaa ccatccccag caataacagg ctgtattgcc
    67321 cgcgtgcact ggctttttcc agatgcgcca gtccgtgctg attacgcagc tcaatggtgt
    67381 cgaccagctg actgacttcg ctactaaact gcgcgaactg ggcgatgtta ttttgtgcga
    67441 gagtttgtag gtgattgctg atttcactgt cctgctgata cagcgccagc aaatcgctat
    67501 attggctaac ggtagttaac gttgttgcga cctgcgcacg aacacccgga tcttcaatgc
    67561 gtatttgccg acgttgcaga attttcaccg cattattgag ctgcttttcc agcgttggtg
    67621 catttttctg gatctgctcc agccccagat tcatcaccat ttgctgcacc cgcagagcgc
    67681 taaggcgcag ttcattcatc tggttaacat actcaagatc gatatcaatc agccgatcga
    67741 gtgcactttc agcagcctga cgctgatctt gttcgatcaa atcgtaaatc ccggcctggg
    67801 tcgctccagc ggaagttgtc gcattattcg cctgaccttg cgccaggcgt gcgatctcat
    67861 cggcggcagc gactatctgc tgactgagtt gccgttgttg ctggcgtagt tgcagacgcc
    67921 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
    67981 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
    68041 tttgtgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
    68101 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
    68161 tggtaggaat caatgcctga gtgttgtctt tttccacctg gctgataaag cgcaggttgt
    68221 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
    68281 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
    68341 aaagcggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
    68401 tatttttact tctttccctt ttcatgttat cggcattttc ggctgataac ctgttgcgct
    68461 ggcatgatgc gcagcattac acggtgcaag cctctacgcc gcttaaagcc aaacgcgcat
    68521 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
    68581 gtatgcagga ggcagctcgc cgctatggtg tggatttaaa agtgctggag gcaggcggct
    68641 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
    68701 ccattttgct cggtagtagc actacgtcat ttcccgacct gcaaaagcag gtagcaaatc
    68761 tgccggtgat cgaactggta aatgctcttg atgatcccct ggtgaaaagc cgcgttggtg
    68821 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
    68881 aaccactgaa cgtgctgttg atgcccggac ccgataatgc cgggggcagt aaagagatgg
    68941 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
    69001 gtgataacga tattgaaatc cagcgtaacc tgttgcagga gatgctggaa cgccacccag
    69061 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
    69121 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
    69181 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
    69241 cggttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
    69301 cgattttagt tctgacgccg aaaaatgccg accgtgaaca tattcgccgc tcgctgtcac
    69361 cagggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
    69421 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
    69481 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgtagcagac
    69541 gttcacggct gagaatttcg cccggattcg tcacaaatgc caccaacatt tcatactctg
    69601 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
    69661 aataaccggc aaagcgatag cagttgtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
    69721 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
    69781 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
    69841 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
    69901 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
    69961 tttcccgcag cccggcaccg ctcgctgtaa cggaaacggt atacccctcc tgagtgaagt
    70021 aagattgtaa tcgcgcctgg gtaaccggct catcttcaac aataacaatg tgatgtggca
    70081 tcagagggtt ttactcattc tgttcatatc tgttcatatt ctgccgtaag ccgttcatcc
    70141 tgaccagtgc cgctgttcat atttgctcat taagatcgct tcactaaacc ataattttac
    70201 aggggctatt atgcggaaac tctggaacgc gctacgccga cccagtgctc gttggtcggt
    70261 actggcgctg gtcgcaattg ggattgtgat tggcattgcg ctgattgtat tgccacacgt
    70321 tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt tgccacagta tgcaaccggt
    70381 gtatgaagaa tataaacagt cggtgcattt ccagaacgcc tccggcgtgc gagctgaatg
    70441 ccatgactgt catatcccgc cggatattcc aggcatggtg aagcgcaaac tggaagcgag
    70501 caatgatatc taccagacct ttattgctca ctccattgat acacctgaaa aattcgaagc
    70561 caaacgcgcg gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca actcggcaac
    70621 ctgccgctcc tgccataact acgatgcgat ggatcatgcg aagcagcatc ctgaagcggc
    70681 acgtcagatg aaggtggcag cgaaagataa tcaatcctgc atcgactgtc ataaaggtat
    70741 tgcccaccag ttaccggata tgagtagcgg cttccgtaag cagttcgatg agctgcgcgc
    70801 cagtgctaat gacagtggtg acacgctgta ctctattgat attaagccaa tttatgcggc
    70861 gaaaggcgat aaagaagcct ctggttctct gctgcctgct tcggaagtga aagtccttaa
    70921 acgtgacggc gactggctgc aaattgaaat taccggctgg acggaaagcg ccggacgtca
    70981 gcgtgtactc acccaattcc caggtaaacg catctttgtt gcctcgattc gtggtgatgt
    71041 gcagcagcag gtaaaaacgc tggagaaaac caccgttgcc gacaccaata ccgagtggag
    71101 caagttgcag gccactgcgt ggatgaagaa aggcgacatg gtgaacgata tcaaaccgat
    71161 ctgggcttat gcggattcgt tgtacaacgg cacctgtaac cagtgccacg gcgcaccgga
    71221 aatcgcccac tttgacgcta acggttggat cggcacgctc aacggcatga ttggctttac
    71281 cagtctcgat aaacgtgaag aacgcacctt gttgaaatat ctgcaaatga atgcgtctga
    71341 caccgcaggt aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa taacgatctc
    71401 tttcaggcat cacgtcggcg ttttctggca caactcggcg gcttaaccgt cgccgggatg
    71461 ctggggccgt cattgttaac gccgcgccgt gcgactgcgg cgcaagcggc gactgacgct
    71521 gtcatctcga aagagggcat tcttaccggg tcgcactggg gggctatccg cgcgacggtg
    71581 aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc gtcgaaaatg
    71641 attgccggat tgccggatca cgtacacaac gcggcgcgta ttcgttatcc gatggtacgc
    71701 gtggactggc tgcgtaagcg ccatctcagc gatacctccc agcgcggtga taaccgtttt
    71761 gtgcgcgtga gctgggatga agccctcgac atgttctatg aagaactgga acgcgtgcag
    71821 aaaactcacg ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac ggggatgttc
    71881 cataacgctt cggggatgct ggcgaaagct attgccttgc atggtaatag cgttggtacg
    71941 ggcggagatt actctaccgg tgctgcgcag gtgatcctgc cgcgcgtggt tggctcaatg
    72001 gaagtgtatg aacagcaaac ctcctggccg ctggtattgc agaacagcaa aaccattgtg
    72061 ctgtggggct ctgatttgct gaaaaaccag caagcgaact ggtggtgccc ggatcacgat
    72121 gtttatgaat attacgcgca gctaaaagcg aaagtcgccg ccggtgaaat tgaggtcatc
    72181 agcatcgatc cggttgtcac atccacccat gagtatctgg ggcgcgagca tgtgaagcac
    72241 attgcggtta acccgcaaac tgatgtgccg ctgcaactgg cgctggcgta tacgctgtac
    72301 agtgaaaacc tgtacgacaa aaacttcctc gctaactact gtgtgggttt tgagcagttc
    72361 ctgccgtacc tgctgggtga gaaagacggt cagccgaaag atgccgcatg ggctgaaaaa
    72421 ctgaccggca ttgatgccga aagcattcgt gggctggcgc ggcagatggc ggcgaacaga
    72481 acgcaaatta ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca gtgggcgtgg
    72541 atgattgtgg ttctggcggc gatgctgggg caaattggcc tgccaggtgg tggctttggt
    72601 tttggctggc actataacgg cgcaggcacg ccggggcgta aaggcgttat tctgagtggt
    72661 ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgattacaa aggctacagc
    72721 agcaccattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa agtgatcaac
    72781 tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc cggaactaac
    72841 ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa gctggaaacg
    72901 gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat cgtgctgcct
    72961 gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc caaccgtggc
    73021 attatcgcca tgaaacaggt ggtgccgccg cagttcgagg cgcgcaacga ctttgatatt
    73081 ttccgcgagc tgtgccgtcg ctttaatcgc gaagaagcct ttaccgaagg gctggacgaa
    73141 atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg acgcggcgtt
    73201 catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt tgaccatccg
    73261 cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc gctgggcacg
    73321 ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta cgacgattgt
    73381 caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc cggctcgcag
    73441 aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc gcagttatgt
    73501 gagtcggaaa ctctgcgtca gcaatatacg gtagcgggta aagagccagt gttcattaac
    73561 ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt ctttaacgct
    73621 cgcggtcagg tgttggcagg ggcagtagtt tctgaccgct atgcacccgg cgtggcacga
    73681 attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc gctgtgcaaa
    73741 tacggtaacc ccaacgtgct gaccatcgac atcggtactt cgcagctggc gcaggcgacc
    73801 agtgcgcaca ctacgctggt ggaaattgag aagtacaatg gagcagtgga gcaggtgacg
    73861 gcgtttaacg gccctgtgga gatggtggcg cagtgcgaat atgttcccgc gtcgcaggtg
    73921 aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg ctggcgcagt
    73981 tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg cagatggctg
    74041 aatggttttc gttgctgaaa agcgaaccgc cgctggctgc ggcggtgaat gagctggaaa
    74101 actgtattgc cacgctgaca gtacgtgatg atgcccgtct ggaactggcc gctgactttt
    74161 gcggcctgtt tctgatgacc gacaaacaag cggcgctgcc gtatgcatcg gcctacaaac
    74221 aggacgagca agagattaaa cgcttgttag ttgaggcagg gatggaaacc agcggcaatt
    74281 tcaacgaacc ggcagatcat ctggcgatct atctcgaatt gctcagccat ctgcattttt
    74341 cgctgggaga ggggaccgtt cctgcgcgaa gaatcgacag tttgcggcaa aaaacactga
    74401 cggcgctgtg gcaatggtta ccagagtttg ttgtgcgttg tcgtcagtat gacagctttg
    74461 gtttttacgc ggcactaagc cagttattgc tggtgttagt ggagagcgac caccaaaaca
    74521 gataacgtcg tttgtgcgcc tgaaaagacg cgtttagcgt cgcatcaggc attatggggc
    74581 agttgccgga tgcgtcatca catccggcaa acacacatca cggatgagct acaaaccggg
    74641 aaagtcgctg gcgcagcagg cggttttcct gcttcaggtg cgcaatatca tccattaacg
    74701 tcagcgccac cgcgatccca ggccagtcca gagccagttc atgacgcagg cgtactgcgc
    74761 gttgcaccac gatggcggca tggtcgtcaa atacccaggt tgtttcctgg atctcacgcg
    74821 gttcaaccac ccccaaaccg acaatttcat tcaactcctc ttcagagatg ccggtatgca
    74881 ggcaaaattc ggtaatagta aaagtcaccg taacattagc cattatgctt tcccccaatc
    74941 tttacgtgga tcaaaagacg actgggcgtc tgccagttgc tgccacagcg cggcagtgtt
    75001 ttcatccggt ttcggcggca tcacgatttt cagtaccgca tacagatcgc cggtctgttt
    75061 tttgctcacc agacctttgc ctttaacgcg caatcgttgc ccggcctggc tgcctggcgg
    75121 gatagtcagc aaaatgcttt ctttcagtgt tggaacggtg actttagcac ccagcgccgc
    75181 ttcccacggg ctaaccggca ccacaatttc cagatcctgg ccgacaatat cgaacagcgg
    75241 atgtggcgca atatgaatca ccagccacaa atcgccattt ggaccaccgt tttcgcccgg
    75301 cgtcccctgg cctttcagac ggatgcgttg accattgccg acgcccgccg ggatcttcac
    75361 attcagcgtt ttcgggattt cctgttcgat catgccaaag gcgttataaa ccggcaggtt
    75421 atagctgatg gtacgcttat gctcagtcag cgtttcttcg aggaataccg ccacttcgat
    75481 ttcaatatcg tggccgcgtg cggcggggcg ttgacggctc tggcgggcat gctgaccgaa
    75541 aattgacgag aagatatcgt caaaatcttc ggcattaaaa ctctgaccgt cgccatgatg
    75601 gaactgacgg ttaaattgcg gatcgttgcg atgttgccac atctgatcat actcagcgcg
    75661 acgttgttca tcacttaaca cttcccaggc ttcagcgacc tctttgaagc gggcttcggc
    75721 atccggttct ttgctgacat caggatggta tttacgggcc agtcgacgat aggcggtctt
    75781 gattgtcttg agatcgtccg tcggtttcac gcccatgatg gcgtaataat cctttaattc
    75841 catagcgtta tctcgcgtaa atcaacacaa attgaaggaa cccctgtaag gtaactccta
    75901 taagtgtagg gtaatcctca aaatttcata tgccaacaca gaatatgtta ttgaaatcat
    75961 cgcggagagg aggtcgccat caagatgggt tgctgaacat attttaaaca ggtgaaaaag
    76021 ggtgagcgat ttttgatagt tgaaccaggc actttaagtt taactggggc gtcattattt
    76081 attaaatttt atagacgcta tatatgggta gtaatataca tggaattagt tgcactgcaa
    76141 ataattattt gaaacaggcc tggaacgata taaaaaatga gtacgaaaaa aatcaaacat
    76201 attcaatcac gctttttgaa aacacactgg tgtgttttat gcggttatac aatgaactca
    76261 gacgtaaagt aaatgaagag gatactccat gtctggaatg tgaatcacta gaaaaagaat
    76321 ttgaggaaat gcagaatgat aatgatctat cattatttat gagaacattg cgtactaatg
    76381 atacacaaat ttattcaggg gtttcaggag gtattacata tactatacaa tatgttcaaa
    76441 atgttgatat tgttagagtg tccttgccgg gcagaggttc agagtctatc acagatttta
    76501 aaggttatta ttggtatggc tttatggaat atattgaaaa cattactgcg tgtgatgatg
    76561 ttttttctga gtattgtttt gatgatgaaa atatgagtat ccagccagaa cagataaata
    76621 cgccgggaat atctgattta gatactggta ttgatttgtc tggcatatct tttattcaga
//
LOCUS       DAZGAN020000009        75599 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGAN020000009 DAZGAN020000000
VERSION     DAZGAN020000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 75599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.009"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..232
                     /locus_tag="ACHKYO_RS03485"
                     /old_locus_tag="pgaptmp_000697"
     CDS             <1..232
                     /locus_tag="ACHKYO_RS03485"
                     /old_locus_tag="pgaptmp_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU4427591.1"
                     /translation="QSTIFLPPGSIFTPVKSGIILKDKEYPITIYNIAPFNKDLWSLL
                     KSSQECPPGESKITNKCLHNSCIIKICPYGLK"
     gene            complement(335..1585)
                     /gene="icd"
                     /locus_tag="ACHKYO_RS03490"
                     /old_locus_tag="pgaptmp_000698"
     CDS             complement(335..1585)
                     /gene="icd"
                     /locus_tag="ACHKYO_RS03490"
                     /old_locus_tag="pgaptmp_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HGU4427592.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1757..2410
                     /gene="rluE"
                     /locus_tag="ACHKYO_RS03495"
                     /old_locus_tag="pgaptmp_000699"
     CDS             1757..2410
                     /gene="rluE"
                     /locus_tag="ACHKYO_RS03495"
                     /old_locus_tag="pgaptmp_000699"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HGU4427593.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2420..2881
                     /gene="nudJ"
                     /locus_tag="ACHKYO_RS03500"
                     /old_locus_tag="pgaptmp_000700"
     CDS             2420..2881
                     /gene="nudJ"
                     /locus_tag="ACHKYO_RS03500"
                     /old_locus_tag="pgaptmp_000700"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HGU4427594.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2935..4041
                     /gene="mnmA"
                     /locus_tag="ACHKYO_RS03505"
                     /old_locus_tag="pgaptmp_000701"
                     /gene_synonym="trmU"
     CDS             2935..4041
                     /gene="mnmA"
                     /locus_tag="ACHKYO_RS03505"
                     /old_locus_tag="pgaptmp_000701"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGU4427595.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4077..4718
                     /gene="hflD"
                     /locus_tag="ACHKYO_RS03510"
                     /old_locus_tag="pgaptmp_000702"
     CDS             4077..4718
                     /gene="hflD"
                     /locus_tag="ACHKYO_RS03510"
                     /old_locus_tag="pgaptmp_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HGU4427596.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4722..6092
                     /gene="purB"
                     /locus_tag="ACHKYO_RS03515"
                     /old_locus_tag="pgaptmp_000703"
     CDS             4722..6092
                     /gene="purB"
                     /locus_tag="ACHKYO_RS03515"
                     /old_locus_tag="pgaptmp_000703"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGU4427597.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6260..6931
                     /gene="phoP"
                     /locus_tag="ACHKYO_RS03520"
                     /old_locus_tag="pgaptmp_000704"
     CDS             6260..6931
                     /gene="phoP"
                     /locus_tag="ACHKYO_RS03520"
                     /old_locus_tag="pgaptmp_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HGU4427598.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6931..8391
                     /gene="phoQ"
                     /locus_tag="ACHKYO_RS03525"
                     /old_locus_tag="pgaptmp_000705"
     CDS             6931..8391
                     /gene="phoQ"
                     /locus_tag="ACHKYO_RS03525"
                     /old_locus_tag="pgaptmp_000705"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HGU4427599.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8467..9588
                     /gene="roxA"
                     /locus_tag="ACHKYO_RS03530"
                     /old_locus_tag="pgaptmp_000706"
                     /gene_synonym="ycfD"
     CDS             8467..9588
                     /gene="roxA"
                     /locus_tag="ACHKYO_RS03530"
                     /old_locus_tag="pgaptmp_000706"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HGU4427600.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9734..10963)
                     /gene="pepT"
                     /locus_tag="ACHKYO_RS03535"
                     /old_locus_tag="pgaptmp_000707"
     CDS             complement(9734..10963)
                     /gene="pepT"
                     /locus_tag="ACHKYO_RS03535"
                     /old_locus_tag="pgaptmp_000707"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGU4427601.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            11213..12349
                     /gene="potA"
                     /locus_tag="ACHKYO_RS03540"
                     /old_locus_tag="pgaptmp_000708"
     CDS             11213..12349
                     /gene="potA"
                     /locus_tag="ACHKYO_RS03540"
                     /old_locus_tag="pgaptmp_000708"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HGU4427602.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12333..13190
                     /gene="potB"
                     /locus_tag="ACHKYO_RS03545"
                     /old_locus_tag="pgaptmp_000709"
     CDS             12333..13190
                     /gene="potB"
                     /locus_tag="ACHKYO_RS03545"
                     /old_locus_tag="pgaptmp_000709"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HGU4427603.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13187..13981
                     /gene="potC"
                     /locus_tag="ACHKYO_RS03550"
                     /old_locus_tag="pgaptmp_000710"
     CDS             13187..13981
                     /gene="potC"
                     /locus_tag="ACHKYO_RS03550"
                     /old_locus_tag="pgaptmp_000710"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HGU4427604.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13978..15024
                     /gene="potD"
                     /locus_tag="ACHKYO_RS03555"
                     /old_locus_tag="pgaptmp_000711"
     CDS             13978..15024
                     /gene="potD"
                     /locus_tag="ACHKYO_RS03555"
                     /old_locus_tag="pgaptmp_000711"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HGU4427605.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            15082..15543
                     /locus_tag="ACHKYO_RS03560"
                     /old_locus_tag="pgaptmp_000712"
     CDS             15082..15543
                     /locus_tag="ACHKYO_RS03560"
                     /old_locus_tag="pgaptmp_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HGU4427606.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            15540..16328
                     /gene="ycfZ"
                     /locus_tag="ACHKYO_RS03565"
                     /old_locus_tag="pgaptmp_000713"
     CDS             15540..16328
                     /gene="ycfZ"
                     /locus_tag="ACHKYO_RS03565"
                     /old_locus_tag="pgaptmp_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU4427607.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(16467..17288)
                     /gene="cobB"
                     /locus_tag="ACHKYO_RS03570"
                     /old_locus_tag="pgaptmp_000714"
     CDS             complement(16467..17288)
                     /gene="cobB"
                     /locus_tag="ACHKYO_RS03570"
                     /old_locus_tag="pgaptmp_000714"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HGU4427608.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(17304..18215)
                     /gene="nagK"
                     /locus_tag="ACHKYO_RS03575"
                     /old_locus_tag="pgaptmp_000715"
     CDS             complement(17304..18215)
                     /gene="nagK"
                     /locus_tag="ACHKYO_RS03575"
                     /old_locus_tag="pgaptmp_000715"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGU4427609.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(18244..19488)
                     /gene="lolE"
                     /locus_tag="ACHKYO_RS03580"
                     /old_locus_tag="pgaptmp_000716"
     CDS             complement(18244..19488)
                     /gene="lolE"
                     /locus_tag="ACHKYO_RS03580"
                     /old_locus_tag="pgaptmp_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HGU4427610.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGVNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19488..20189)
                     /gene="lolD"
                     /locus_tag="ACHKYO_RS03585"
                     /old_locus_tag="pgaptmp_000717"
     CDS             complement(19488..20189)
                     /gene="lolD"
                     /locus_tag="ACHKYO_RS03585"
                     /old_locus_tag="pgaptmp_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HGU4427611.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(20182..21381)
                     /gene="lolC"
                     /locus_tag="ACHKYO_RS03590"
                     /old_locus_tag="pgaptmp_000718"
     CDS             complement(20182..21381)
                     /gene="lolC"
                     /locus_tag="ACHKYO_RS03590"
                     /old_locus_tag="pgaptmp_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HGU4427612.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21643..22716
                     /gene="ycfT"
                     /locus_tag="ACHKYO_RS03595"
                     /old_locus_tag="pgaptmp_000719"
     CDS             21643..22716
                     /gene="ycfT"
                     /locus_tag="ACHKYO_RS03595"
                     /old_locus_tag="pgaptmp_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGU4427613.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWSIFWVLTLWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQQFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLGYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAISYSR"
     gene            22844..26290
                     /gene="mfd"
                     /locus_tag="ACHKYO_RS03600"
                     /old_locus_tag="pgaptmp_000720"
     CDS             22844..26290
                     /gene="mfd"
                     /locus_tag="ACHKYO_RS03600"
                     /old_locus_tag="pgaptmp_000720"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGU4427614.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDKLFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26434..27396
                     /gene="ldtC"
                     /locus_tag="ACHKYO_RS03605"
                     /old_locus_tag="pgaptmp_000721"
     CDS             26434..27396
                     /gene="ldtC"
                     /locus_tag="ACHKYO_RS03605"
                     /old_locus_tag="pgaptmp_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HGU4427615.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27479..27736)
                     /gene="bhsA"
                     /locus_tag="ACHKYO_RS03610"
                     /old_locus_tag="pgaptmp_000722"
                     /gene_synonym="ycfR"
     CDS             complement(27479..27736)
                     /gene="bhsA"
                     /locus_tag="ACHKYO_RS03610"
                     /old_locus_tag="pgaptmp_000722"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HGU4427616.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            27977..28609
                     /gene="comR"
                     /locus_tag="ACHKYO_RS03615"
                     /old_locus_tag="pgaptmp_000723"
     CDS             27977..28609
                     /gene="comR"
                     /locus_tag="ACHKYO_RS03615"
                     /old_locus_tag="pgaptmp_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HGU4427617.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(28671..29210)
                     /gene="ycfJ"
                     /locus_tag="ACHKYO_RS03620"
                     /old_locus_tag="pgaptmp_000724"
     CDS             complement(28671..29210)
                     /gene="ycfJ"
                     /locus_tag="ACHKYO_RS03620"
                     /old_locus_tag="pgaptmp_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU4427618.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(29420..30724)
                     /gene="ndh"
                     /locus_tag="ACHKYO_RS03625"
                     /old_locus_tag="pgaptmp_000725"
     CDS             complement(29420..30724)
                     /gene="ndh"
                     /locus_tag="ACHKYO_RS03625"
                     /old_locus_tag="pgaptmp_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HGU4427619.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(31132..31674)
                     /gene="ycfP"
                     /locus_tag="ACHKYO_RS03630"
                     /old_locus_tag="pgaptmp_000726"
     CDS             complement(31132..31674)
                     /gene="ycfP"
                     /locus_tag="ACHKYO_RS03630"
                     /old_locus_tag="pgaptmp_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HGU4427620.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(31697..32722)
                     /gene="nagZ"
                     /locus_tag="ACHKYO_RS03635"
                     /old_locus_tag="pgaptmp_000727"
     CDS             complement(31697..32722)
                     /gene="nagZ"
                     /locus_tag="ACHKYO_RS03635"
                     /old_locus_tag="pgaptmp_000727"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGU4427621.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(32733..33557)
                     /gene="thiK"
                     /locus_tag="ACHKYO_RS03640"
                     /old_locus_tag="pgaptmp_000728"
     CDS             complement(32733..33557)
                     /gene="thiK"
                     /locus_tag="ACHKYO_RS03640"
                     /old_locus_tag="pgaptmp_000728"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HGU4427622.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(33538..34179)
                     /gene="lpoB"
                     /locus_tag="ACHKYO_RS03645"
                     /old_locus_tag="pgaptmp_000729"
     CDS             complement(33538..34179)
                     /gene="lpoB"
                     /locus_tag="ACHKYO_RS03645"
                     /old_locus_tag="pgaptmp_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HGU4427623.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(34193..34570)
                     /gene="ycfL"
                     /locus_tag="ACHKYO_RS03650"
                     /old_locus_tag="pgaptmp_000730"
     CDS             complement(34193..34570)
                     /gene="ycfL"
                     /locus_tag="ACHKYO_RS03650"
                     /old_locus_tag="pgaptmp_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HGU4427624.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(34573..34932)
                     /gene="hinT"
                     /locus_tag="ACHKYO_RS03655"
                     /old_locus_tag="pgaptmp_000731"
     CDS             complement(34573..34932)
                     /gene="hinT"
                     /locus_tag="ACHKYO_RS03655"
                     /old_locus_tag="pgaptmp_000731"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HGU4427625.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            35266..37455
                     /gene="fhuE"
                     /locus_tag="ACHKYO_RS03660"
                     /old_locus_tag="pgaptmp_000732"
     CDS             35266..37455
                     /gene="fhuE"
                     /locus_tag="ACHKYO_RS03660"
                     /old_locus_tag="pgaptmp_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HGU4427626.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGIYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITGTYQF"
     gene            complement(37515..38948)
                     /gene="ptsG"
                     /locus_tag="ACHKYO_RS03665"
                     /old_locus_tag="pgaptmp_000733"
     CDS             complement(37515..38948)
                     /gene="ptsG"
                     /locus_tag="ACHKYO_RS03665"
                     /old_locus_tag="pgaptmp_000733"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HGU4427627.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(39243..40040)
                     /gene="ycfH"
                     /locus_tag="ACHKYO_RS03670"
                     /old_locus_tag="pgaptmp_000734"
     CDS             complement(39243..40040)
                     /gene="ycfH"
                     /locus_tag="ACHKYO_RS03670"
                     /old_locus_tag="pgaptmp_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4427628.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(40051..41055)
                     /gene="holB"
                     /locus_tag="ACHKYO_RS03675"
                     /old_locus_tag="pgaptmp_000735"
     CDS             complement(40051..41055)
                     /gene="holB"
                     /locus_tag="ACHKYO_RS03675"
                     /old_locus_tag="pgaptmp_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGU4427629.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(41052..41693)
                     /gene="tmk"
                     /locus_tag="ACHKYO_RS03680"
                     /old_locus_tag="pgaptmp_000736"
     CDS             complement(41052..41693)
                     /gene="tmk"
                     /locus_tag="ACHKYO_RS03680"
                     /old_locus_tag="pgaptmp_000736"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4427630.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(41683..42705)
                     /gene="yceG"
                     /locus_tag="ACHKYO_RS03685"
                     /old_locus_tag="pgaptmp_000737"
     CDS             complement(41683..42705)
                     /gene="yceG"
                     /locus_tag="ACHKYO_RS03685"
                     /old_locus_tag="pgaptmp_000737"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HGU4427631.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(42708..43517)
                     /gene="pabC"
                     /locus_tag="ACHKYO_RS03690"
                     /old_locus_tag="pgaptmp_000738"
     CDS             complement(42708..43517)
                     /gene="pabC"
                     /locus_tag="ACHKYO_RS03690"
                     /old_locus_tag="pgaptmp_000738"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HGU4427632.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(43637..44878)
                     /gene="fabF"
                     /locus_tag="ACHKYO_RS03695"
                     /old_locus_tag="pgaptmp_000739"
     CDS             complement(43637..44878)
                     /gene="fabF"
                     /locus_tag="ACHKYO_RS03695"
                     /old_locus_tag="pgaptmp_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGU4427633.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(44966..45202)
                     /gene="acpP"
                     /locus_tag="ACHKYO_RS03700"
                     /old_locus_tag="pgaptmp_000740"
     CDS             complement(44966..45202)
                     /gene="acpP"
                     /locus_tag="ACHKYO_RS03700"
                     /old_locus_tag="pgaptmp_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4427634.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(45413..46147)
                     /gene="fabG"
                     /locus_tag="ACHKYO_RS03705"
                     /old_locus_tag="pgaptmp_000741"
     CDS             complement(45413..46147)
                     /gene="fabG"
                     /locus_tag="ACHKYO_RS03705"
                     /old_locus_tag="pgaptmp_000741"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HGU4427635.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(46160..47089)
                     /gene="fabD"
                     /locus_tag="ACHKYO_RS03710"
                     /old_locus_tag="pgaptmp_000742"
     CDS             complement(46160..47089)
                     /gene="fabD"
                     /locus_tag="ACHKYO_RS03710"
                     /old_locus_tag="pgaptmp_000742"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGU4427636.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(47105..48058)
                     /gene="fabH"
                     /locus_tag="ACHKYO_RS03715"
                     /old_locus_tag="pgaptmp_000743"
     CDS             complement(47105..48058)
                     /gene="fabH"
                     /locus_tag="ACHKYO_RS03715"
                     /old_locus_tag="pgaptmp_000743"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGU4427637.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(48126..49196)
                     /gene="plsX"
                     /locus_tag="ACHKYO_RS03720"
                     /old_locus_tag="pgaptmp_000744"
     CDS             complement(48126..49196)
                     /gene="plsX"
                     /locus_tag="ACHKYO_RS03720"
                     /old_locus_tag="pgaptmp_000744"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGU4427638.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(49277..49450)
                     /gene="rpmF"
                     /locus_tag="ACHKYO_RS03725"
                     /old_locus_tag="pgaptmp_000745"
     CDS             complement(49277..49450)
                     /gene="rpmF"
                     /locus_tag="ACHKYO_RS03725"
                     /old_locus_tag="pgaptmp_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGU4427639.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(49502..50023)
                     /gene="yceD"
                     /locus_tag="ACHKYO_RS03730"
                     /old_locus_tag="pgaptmp_000746"
     CDS             complement(49502..50023)
                     /gene="yceD"
                     /locus_tag="ACHKYO_RS03730"
                     /old_locus_tag="pgaptmp_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HGU4427640.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            50222..50806
                     /gene="yceF"
                     /locus_tag="ACHKYO_RS03735"
                     /old_locus_tag="pgaptmp_000747"
     CDS             50222..50806
                     /gene="yceF"
                     /locus_tag="ACHKYO_RS03735"
                     /old_locus_tag="pgaptmp_000747"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HGU4427641.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(50918..51877)
                     /gene="rluC"
                     /locus_tag="ACHKYO_RS03740"
                     /old_locus_tag="pgaptmp_000748"
     CDS             complement(50918..51877)
                     /gene="rluC"
                     /locus_tag="ACHKYO_RS03740"
                     /old_locus_tag="pgaptmp_000748"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HGU4427642.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            52450..55635
                     /gene="rne"
                     /locus_tag="ACHKYO_RS03745"
                     /old_locus_tag="pgaptmp_000749"
     CDS             52450..55635
                     /gene="rne"
                     /locus_tag="ACHKYO_RS03745"
                     /old_locus_tag="pgaptmp_000749"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HGU4427643.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(55832..56785)
                     /gene="flgL"
                     /locus_tag="ACHKYO_RS03750"
                     /old_locus_tag="pgaptmp_000750"
     CDS             complement(55832..56785)
                     /gene="flgL"
                     /locus_tag="ACHKYO_RS03750"
                     /old_locus_tag="pgaptmp_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HGU4427644.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(56797..58440)
                     /gene="flgK"
                     /locus_tag="ACHKYO_RS03755"
                     /old_locus_tag="pgaptmp_000751"
     CDS             complement(56797..58440)
                     /gene="flgK"
                     /locus_tag="ACHKYO_RS03755"
                     /old_locus_tag="pgaptmp_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HGU4427645.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTATPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(58506..59447)
                     /gene="flgJ"
                     /locus_tag="ACHKYO_RS03760"
                     /old_locus_tag="pgaptmp_000752"
     CDS             complement(58506..59447)
                     /gene="flgJ"
                     /locus_tag="ACHKYO_RS03760"
                     /old_locus_tag="pgaptmp_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HGU4427646.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(59447..60544)
                     /gene="flgI"
                     /locus_tag="ACHKYO_RS03765"
                     /old_locus_tag="pgaptmp_000753"
     CDS             complement(59447..60544)
                     /gene="flgI"
                     /locus_tag="ACHKYO_RS03765"
                     /old_locus_tag="pgaptmp_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HGU4427647.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(60556..61254)
                     /gene="flgH"
                     /locus_tag="ACHKYO_RS03770"
                     /old_locus_tag="pgaptmp_000754"
     CDS             complement(60556..61254)
                     /gene="flgH"
                     /locus_tag="ACHKYO_RS03770"
                     /old_locus_tag="pgaptmp_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HGU4427648.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQILVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(61307..62089)
                     /gene="flgG"
                     /locus_tag="ACHKYO_RS03775"
                     /old_locus_tag="pgaptmp_000755"
     CDS             complement(61307..62089)
                     /gene="flgG"
                     /locus_tag="ACHKYO_RS03775"
                     /old_locus_tag="pgaptmp_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGU4427649.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(62227..62982)
                     /gene="flgF"
                     /locus_tag="ACHKYO_RS03780"
                     /old_locus_tag="pgaptmp_000756"
     CDS             complement(62227..62982)
                     /gene="flgF"
                     /locus_tag="ACHKYO_RS03780"
                     /old_locus_tag="pgaptmp_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HGU4427650.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(63002..64207)
                     /gene="flgE"
                     /locus_tag="ACHKYO_RS03785"
                     /old_locus_tag="pgaptmp_000757"
     CDS             complement(63002..64207)
                     /gene="flgE"
                     /locus_tag="ACHKYO_RS03785"
                     /old_locus_tag="pgaptmp_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HGU4427651.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(64232..64927)
                     /gene="flgD"
                     /locus_tag="ACHKYO_RS03790"
                     /old_locus_tag="pgaptmp_000758"
     CDS             complement(64232..64927)
                     /gene="flgD"
                     /locus_tag="ACHKYO_RS03790"
                     /old_locus_tag="pgaptmp_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HGU4427652.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNRGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(64939..65343)
                     /gene="flgC"
                     /locus_tag="ACHKYO_RS03795"
                     /old_locus_tag="pgaptmp_000759"
     CDS             complement(64939..65343)
                     /gene="flgC"
                     /locus_tag="ACHKYO_RS03795"
                     /old_locus_tag="pgaptmp_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HGU4427653.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(65347..65763)
                     /gene="flgB"
                     /locus_tag="ACHKYO_RS03800"
                     /old_locus_tag="pgaptmp_000760"
     CDS             complement(65347..65763)
                     /gene="flgB"
                     /locus_tag="ACHKYO_RS03800"
                     /old_locus_tag="pgaptmp_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HGU4427654.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            65918..66577
                     /gene="flgA"
                     /locus_tag="ACHKYO_RS03805"
                     /old_locus_tag="pgaptmp_000761"
     CDS             65918..66577
                     /gene="flgA"
                     /locus_tag="ACHKYO_RS03805"
                     /old_locus_tag="pgaptmp_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HGU4427655.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            66653..66946
                     /gene="flgM"
                     /locus_tag="ACHKYO_RS03810"
                     /old_locus_tag="pgaptmp_000762"
     CDS             66653..66946
                     /gene="flgM"
                     /locus_tag="ACHKYO_RS03810"
                     /old_locus_tag="pgaptmp_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGU4427656.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            66951..67367
                     /gene="flgN"
                     /locus_tag="ACHKYO_RS03815"
                     /old_locus_tag="pgaptmp_000763"
     CDS             66951..67367
                     /gene="flgN"
                     /locus_tag="ACHKYO_RS03815"
                     /old_locus_tag="pgaptmp_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HGU4427657.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANAQTSTTHRGGKKISI"
     gene            complement(67407..68942)
                     /gene="murJ"
                     /locus_tag="ACHKYO_RS03820"
                     /old_locus_tag="pgaptmp_000764"
     CDS             complement(67407..68942)
                     /gene="murJ"
                     /locus_tag="ACHKYO_RS03820"
                     /old_locus_tag="pgaptmp_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGU4427658.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(69052..69975)
                     /gene="yceM"
                     /locus_tag="ACHKYO_RS03825"
                     /old_locus_tag="pgaptmp_000765"
     CDS             complement(69052..69975)
                     /gene="yceM"
                     /locus_tag="ACHKYO_RS03825"
                     /old_locus_tag="pgaptmp_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4427659.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(69977..70624)
                     /gene="yceH"
                     /locus_tag="ACHKYO_RS03830"
                     /old_locus_tag="pgaptmp_000766"
     CDS             complement(69977..70624)
                     /gene="yceH"
                     /locus_tag="ACHKYO_RS03830"
                     /old_locus_tag="pgaptmp_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HGU4427660.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(70635..71219)
                     /gene="rimJ"
                     /locus_tag="ACHKYO_RS03835"
                     /old_locus_tag="pgaptmp_000767"
     CDS             complement(70635..71219)
                     /gene="rimJ"
                     /locus_tag="ACHKYO_RS03835"
                     /old_locus_tag="pgaptmp_000767"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HGU4427661.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            71455..72663
                     /gene="mdtH"
                     /locus_tag="ACHKYO_RS03840"
                     /old_locus_tag="pgaptmp_000768"
     CDS             71455..72663
                     /gene="mdtH"
                     /locus_tag="ACHKYO_RS03840"
                     /old_locus_tag="pgaptmp_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HGU4427662.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVHTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            72727..73374
                     /gene="grxB"
                     /locus_tag="ACHKYO_RS03845"
                     /old_locus_tag="pgaptmp_000769"
     CDS             72727..73374
                     /gene="grxB"
                     /locus_tag="ACHKYO_RS03845"
                     /old_locus_tag="pgaptmp_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HGU4427663.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            73508..74068
                     /gene="yceB"
                     /locus_tag="ACHKYO_RS03850"
                     /old_locus_tag="pgaptmp_000770"
     CDS             73508..74068
                     /gene="yceB"
                     /locus_tag="ACHKYO_RS03850"
                     /old_locus_tag="pgaptmp_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4427664.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            74174..75220
                     /gene="pyrC"
                     /locus_tag="ACHKYO_RS03855"
                     /old_locus_tag="pgaptmp_000771"
     CDS             74174..75220
                     /gene="pyrC"
                     /locus_tag="ACHKYO_RS03855"
                     /old_locus_tag="pgaptmp_000771"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGU4427665.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            75294..75539
                     /gene="dinI"
                     /locus_tag="ACHKYO_RS03860"
                     /old_locus_tag="pgaptmp_000772"
     CDS             75294..75539
                     /gene="dinI"
                     /locus_tag="ACHKYO_RS03860"
                     /old_locus_tag="pgaptmp_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HGU4427666.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        18374 a        20090 c        18888 g        18247 t
ORIGIN      
        1 tcaatctact atatttctcc ctcctggcag tatatttaca ccggttaaat ctggaattat
       61 ccttaaagat aaagaatatc ctattactat ttataacatc gcaccattca acaaggattt
      121 atggagtcta ctcaaaagca gtcaagagtg tcctccagga gaaagcaaaa taacaaataa
      181 atgtttgcat aatagttgca ttataaaaat atgcccatat gggctcaagt aaagggtttt
      241 atataaatat gctcgatatg ctattaataa tacctacata aataaaacaa cgggcgtgtt
      301 atacgcccgt tgcaatatta aacacatgta gtgattacat gttcttgatg atcgcgtcac
      361 caaattctga acatttcagc agtttagcgc cttccatcag acgctcgaag tcataggtta
      421 cggtcttcgc attgattgcg ccttccatac ctttaacaat caggtctgcg gcttcagtcc
      481 agcccatatg gcgtaacatc atctcagcgg agagaataat agagccaggg tttactttgt
      541 cctgaccggc gtatttcggc gcagtaccgt gggtggcttc aaacagggcg cattcgtcac
      601 cgatgtttgc tccaggggcg ataccgatac cgccaacctg cgctgccaga gcgtcagaaa
      661 tgtagtcacc gttcaggttc atacaggcga taacatcata ttcagccgga cgcagcagga
      721 tctgttgcag gaatgcatca gcaatcacgt ctttaatgac gatctctttg ccggtgttcg
      781 ggtttttaac tttcagccac gggccgccgt cgatcagttc accgccaaac tcttcacgcg
      841 ccagctggta gccccagtct ttaaacgcgc cttcggtgaa cttcatgatg ttgcctttgt
      901 gcaccagagt cacagagtca cgatcgttag caattgcgta ttcgatcgct gcacgaacca
      961 gacgtttggt gccttcttcc gaacacggct taataccgat accacaatgt tccgggaagc
     1021 gaattttctt cacccccatc tcttcacgca ggaatttaat cactttctcg gcgtcggcag
     1081 agtctgcttt ccattcgata cccgcataaa tgtcttccga gttttcacgg aagataacca
     1141 tatcggtcag ttcagggtgt ttaaccgggc ttggagtgcc ctgatagtaa cgtaccggac
     1201 gcaggcagat gtagagatcc agttcctggc gcagggcaac gttcagagag cgaataccgc
     1261 caccaacagg agtggtcagc ggacctttaa tggcaacgcg atattcacga atcagatcaa
     1321 gggtttcagc aggcagccag acatcctgac cataaacctg tgtggatttt tcaccggtgt
     1381 aaatttccat ccaggagatt ttacgctcgc ctttataggc tttctcgact gcagcgtcga
     1441 ccactttcag catggctggg gttacatcta caccgattcc atcaccttca atgtaaggga
     1501 taatcggatt ttcaggaacg ttgagtttgc cgttttgcag ggtgatcttc ttgccttgtg
     1561 ccggaacaac tactttactt tccattcacc tctccttcga gcgctactgg tttgctcgtc
     1621 tgccaccacg ttgcatatgc gtttgcgtcc tgcgatacgg atgctttaga gcaatttttt
     1681 gttaatgatt tgtaattggc ttgtcaatac taccctattg tttgccgtca tgaaagatgc
     1741 gcgttattag gctataatgc ggcaattcat aatctctgaa aataccatgc aaaaaacttc
     1801 ttttagaaat caccaggtta agcgattcag ctcgcaacgt tctaccaggc gtaaacctga
     1861 aaaccagccc acgcgtgtga tcctgttcaa taaaccctac gatgttcttc cgcagttcac
     1921 cgatgaagcc ggacgcaaaa cattaaaaga attcatcccg gttcagggcg tttatgcagc
     1981 aggtcgtctt gaccgcgata gcgaagggtt actggtgctg accaataacg gcgcgttgca
     2041 ggcgcgttta acccagccgg gcaaacgcac cgggaaaatc tattatgtgc aggtggaagg
     2101 tattcccaca caagacgcac ttgaagcctt gcgcaatggc gtaaccttaa atgatggccc
     2161 taccctgccc gcaggcgcgg agctggttga cgaacccgcg tggttatggc cgcggaatcc
     2221 accaattcgt gaacgcaaaa gtattcccac cagctggctg aagatcacct tatatgaagg
     2281 acgtaatcgc caggtgcgcc gcatgaccgc ccatgttggt ttccccacgc tgcgactgat
     2341 tcgctatgcg atgggagatt actctttgga taatcttgcc aatggcgaat ggcgagaagt
     2401 gacagattaa ggatgcaaaa tgtttaaacc gcacgttacc gttgcttgcg tggtgcacgc
     2461 agaaggcaaa tttttagtcg ttgaagagac gattaatggt aaagcgttat ggaaccaacc
     2521 tgccgggcat ctggaagccg atgaaacctt agtggaagcc gccgcccgtg aactgtggga
     2581 agaaaccggc atcagcgcgc agccgcaaca ctttattcgt atgcatcagt ggattgcgcc
     2641 agataaaacg ccgtttttgc gtttcctctt tgccattgag cttgagcaaa tatgcccgac
     2701 ccagcctcat gacagcgata tcgactgctg tcgttgggtc agcgccgaag aaattttaca
     2761 ggcgtcaaat cttcgttcgc cgctggtggc ggaaagtatc cgttgttatc aaagcgggca
     2821 acgctatccg ctggagatga ttggcgattt taactggcct tttacaaagg gtgtcatcta
     2881 aaaggctgcg tgatagaata cgccgccttg aagttcaatg tcgtgagtga tccaatgtct
     2941 gaaaccgcaa aaaaagtaat cgtcggcatg tccggcggtg tcgattcctc cgtttctgcc
     3001 tggctgttgc aacaacaggg atatcaggtt gaaggcctgt ttatgaagaa ctgggaagaa
     3061 gacgacggtg aggaatattg cacagcggca gcggatctgg ctgatgccca ggctgtctgc
     3121 gacaagctcg gcattgaact gcacaccgtt aactttgctg ccgagtactg ggacaacgtc
     3181 ttcgaactgt tccttgccga atataaagcc ggtcgcacgc cgaatccgga tattctgtgc
     3241 aacaaagaga tcaaatttaa agccttcctc gaatttgccg ccgaagattt aggtgccgat
     3301 tatatcgcta ccggtcatta cgtacgtcgg gccgatgtcg atggcaagag ccgcctgctg
     3361 cgtggtctgg acagcaataa agaccagagc tacttccttt atacgctcag ccatgagcag
     3421 attgcgcaaa gcctgttccc ggtcggcgaa ctggaaaaac cgcaggtgcg taagattgct
     3481 gaagatcttg gtctggtcac cgcgaagaaa aaagactcta ccggcatttg cttcattggc
     3541 gaacgtaaat tccgcgagtt cctgggccgt tatctcccgg cgcaaccggg caaaatcatt
     3601 accgtcgatg gcgatgaaat tggtgagcac caggggctga tgtatcacac tctcggtcag
     3661 cgtaaaggtc tgggtatcgg tggcaccaaa gaaggtactg aagaaccgtg gtatgtggtg
     3721 gacaaagacg tcgaaaacaa cattctggtt gtcgctcagg gccatgaaca cccgcggctg
     3781 atgtctgtcg ggttgattgc ccagcagttg cactgggtcg atcgcgaacc attcaccggc
     3841 actatgcgtt gcacggtaaa aacccgctat cgccagaccg atatcccttg caccgtcaag
     3901 gcgctggacg atgatcgcat tgaagtgatt ttcgatgaac cggttgccgc cgtgacgccg
     3961 ggccagtctg ccgtcttcta taacggtgaa gtgtgcctcg gtggcggtat tattgagcag
     4021 cgtctgccgc tgccggtctg attattatct ttacttaaaa caaggaagca gtgaacgtgg
     4081 caaagaatta ctatgacatc accctcgccc tggccggtat ttgtcagtcg gcacgcctgg
     4141 tgcaacaact cgctcaccag gggcattgtg atgccgatgc gctacacgtc tcactcaaca
     4201 gtattattga tatgaacccc agctcgacgc tggcggtttt tggcggtagc gaagccaacc
     4261 tgcgcgtcgg gctggaaacc ctgctcggcg tgctcaatgc cagcagtcgc cagggcttaa
     4321 acgctgagtt aacccgctac acactcagct tgatggtgct tgagcgcaaa ctctcctcag
     4381 cgaaaggcgc gctcgacact ctgggcaacc ggatcaacgg cctgcaacgc cagctcgaac
     4441 acttcgattt acagtccgaa acgctgatga gcgcgatggc tgctatctat gttgatgtga
     4501 tcagcccgct tggcccgcgc attcaggtca ccggttcccc tgctgtactg caaagcccac
     4561 aagtgcaggc gaaagttcgc gcaaccctgc tggcaggcat tcgcgccgcc gtgctctggc
     4621 accaggtcgg cggcggacgt ctgcaactga tgttttctcg taatcgcctg accactcagg
     4681 caaaacaaat tcttgctcat ttaaccccgg agttgtgatc tatggaatta tcctcactga
     4741 ccgccgtttc ccctgtcgat ggacgctacg gcgataaagt cagcgcgctg cgcgggattt
     4801 tcagcgaata tggtttgctg aaattccgtg tacaagttga agtacgttgg ctgcaaaaac
     4861 tggccgcgca cgcagcgatc aaggaagttc ctgcttttgc tgccgacgca atcggttacc
     4921 ttgatgcaat tgtcgccaat ttcagcgaag aagatgccgc acgcatcaaa accatcgagc
     4981 gtaccactaa ccacgacgtt aaagcggttg agtatttcct gaaagaaaaa gtggcggaga
     5041 tcccggaact gcacgcggtt tctgaattca tccactttgc ctgtacttcg gaagatatca
     5101 ataacctctc ccacgcatta atgctgaaaa ccgcgcgtga tgaagtgatc ctgccgtact
     5161 ggcgtcaact gattgatggc attaaagatc tcgccgttca gtaccgcgat atcccgctgc
     5221 tgtctcgtac ccacggtcag ccagccacgc cgtcaaccat cggtaaagag atggcaaacg
     5281 tcgcctaccg tatggagcgc cagtaccgcc agcttaacca ggtggagatc ctcggcaaaa
     5341 tcaacggcgc ggtcggtaac tataacgccc acatcgccgc ttacccggaa gttgactggc
     5401 atcagttcag cgaagagttc gtcacctcgc tgggtattca atggaacccg tacactaccc
     5461 agattgaacc gcacgactac attgccgaac tgtttgattg cgttgcgcgc ttcaacacca
     5521 ttctgatcga ctttgaccgt gacgtctggg gttatatcgc ccttaaccac ttcaaacaga
     5581 aaaccattgc tggtgagatt ggttcttcca ccatgccgca taaagttaac ccgatcgact
     5641 tcgaaaactc cgaagggaat ctgggccttt ccaacgcggt attgcagcat ctggcaagca
     5701 aactgccggt ttcccgctgg cagcgtgacc tgaccgactc caccgtgctg cgtaacctcg
     5761 gcgtgggtat cggttatgcg ctgattgcgt atcaatccac cctgaaaggc gtgagcaaac
     5821 tggaagtgaa ccgtgaccat ctgctggatg agctggatca caactgggaa gtgctggcag
     5881 aaccaattca gacagttatg cgtcgctatg gcatcgaaaa accgtacgag aagctgaaag
     5941 agctgactcg tggtaagcgc gttgacgccg aaggcatgaa gcagtttatc gacggtctgg
     6001 cgttgccaga agaagagaaa gcccgcctga aagcgatgac gccggcaaac tacattggtc
     6061 gcgccatcac catggttgat gagctgaaat aaactcgtat cagtgccgga tggcgatgct
     6121 gtccggcctg cttattaaga ttatccgctt tttatttttt cactttacct cccctccccg
     6181 ctggtttatt taatgtttac ccccataacc acataatcgc gttacactat tttaataatt
     6241 aagacaggga gaaataaaaa tgcgcgtact ggttgttgaa gacaatgcgt tgttacgtca
     6301 ccaccttaaa gttcagattc aggatgctgg tcatcaggtc gatgatgcag aagatgccaa
     6361 agaagccgat tattatctca atgaacattt accggatatt gcgattgtcg atctcggatt
     6421 gccagacgag gacggtctgt cactgattcg ccgctggcgt agcaatgatg tttcactgcc
     6481 gattctggta ttaaccgccc gtgaaagctg gcaggacaaa gtcgaagtat taagtgctgg
     6541 tgctgatgat tacgtgacta aaccatttca tattgaagag gtgatggcgc gaatgcaggc
     6601 attaatgcgg cgtaatagcg gtctggcttc acaggtcatt tcgctaccac cgtttcaggt
     6661 tgatctctct cgccgtgaat tatctattaa tgacgaagtg atcaaactga ccgcgttcga
     6721 atacaccatt atggaaacgt tgatacgcaa taatggcaaa gtggtgagca aagattcgtt
     6781 aatgctccaa ctctatccgg atgcggagct gcgggaaagc cataccattg atgtactgat
     6841 gggacgtctg cgcaaaaaaa ttcaggcaca atatccgcaa gaagtcatta ccaccgttcg
     6901 cggccagggc tacctgttcg aattgcgctg atgaaaaaat tactgcgtct ttttttcccg
     6961 ctctcgctgc gggtacgttt tctgttggca acggcagcgg tagtactggt gctttcgctt
     7021 gcctacggaa tggtcgcgct gatcggttat agcgtcagtt tcgataaaac tacgtttcgg
     7081 ctgttacgtg gcgagagcaa tctgttctat acccttgcga agtgggaaaa caataagttg
     7141 catgtcgagt tacccgaaaa tatcgacaag caaagcccca ccatgacgct aatttatgat
     7201 gagaacgggc agcttttatg ggcgcaacgt gacgtgccct ggctgatgaa gatgatccag
     7261 cctgactggc tgaaatcgaa tggttttcat gaaattgaag cggatgttaa cgataccagc
     7321 ctcttgctga gtggagatca ttcgatacag caacagttgc aggaagtgcg ggaagatgat
     7381 gacgacgcgg agatgaccca ctcggtggcg gtaaacgtct acccggcaac atcgcggatg
     7441 ccaaaattaa ccattgtggt ggtggatacc attccggtgg agctaaaaag ttcctatatg
     7501 gtctggagct ggtttatcta tgtgctctca gccaatctgc tgttagtaat cccgctgctg
     7561 tgggtcgccg cctggtggag tttacgcccc atcgaagccc tggcaaaaga agtccgcgaa
     7621 ctggaagaac ataaccgcga attgctcaat ccagccacaa cgcgagaact gaccagtctg
     7681 gtacgaaacc tgaaccgatt gttaaaaagt gaacgcgaac gttacgacaa ataccgtacg
     7741 acgctcaccg acctgaccca tagtctgaaa acgccactgg cggtgctgca aagtacgctg
     7801 cgttctctgc gtagtgaaaa gatgagcgtc agtgatgctg agccggtaat gctggagcaa
     7861 atcagccgca tttcacagca aattggctac tacctgcatc gtgccagtat gcgcggcggg
     7921 acattgctca gccgcgagct gcatccggtc gccccactgc tggacaatct cacctcagcg
     7981 ctgaacaaag tgtatcaacg caaaggggtc aatatctctc tcgatatttc gccagagatc
     8041 agctttgtcg gtgagcagaa cgattttgtc gaggtgatgg gcaacgtgct ggataatgcc
     8101 tgtaaatatt gcctcgagtt tgtcgaaatt tctgcaaggc aaaccgacga gcatctctat
     8161 attgtggtcg aggatgatgg ccccggtatt ccattaagca agcgagaggt cattttcgac
     8221 cgtggtcaac gggttgatac tttacgccct gggcaaggtg tagggctggc ggtagcccgc
     8281 gaaatcaccg agcaatatga gggtaaaatc gtcgccggag agagcatgct gggcggtgcg
     8341 cggatggagg tgatttttgg tcgccagcat tctgcgccga aagatgaata aatatgtcca
     8401 tacttcacgc attacgttaa gcatccgtta taatcggttg cagataccag cctgtggatg
     8461 cttaacatgg aataccaact cactcttaac tggcccgatt ttcttgaacg tcactggcag
     8521 aaacgcccgg tggtgttaaa acgcggcttt aataatttta ttgacccgat ctctccagac
     8581 gagttggcgg gtctggcgat ggaaagcgaa gttgacagtc gactggtcag tcaccaggat
     8641 ggcaaatggc aggtcagcca cggcccgttc gaaagctacg atcatctcgg tgaaaccaac
     8701 tggtcattac tggtacaggc agtaaaccac tggcatgagc cgaccgccgc gctgatgcga
     8761 ccgttccgtg aactaccgga ctggcgtatt gatgatctga tgatctcttt ttctgtacct
     8821 ggcggcggcg tcggcccgca tctcgatcag tacgacgtgt ttatcattca gggtaccgga
     8881 cgtcgtcgct ggcgagtggg cgaaaagctg caaatgaaac agcactgccc acatccggat
     8941 ctgttacagg tcgatccgtt cgaagccatc atcgatgaag agctggagcc tggtgatatt
     9001 ctttatattc cgccaggatt cccgcatgaa ggctacgcgc tggaaaatgc gatgaactat
     9061 tccgtgggct ttcgcgcgcc aaatacgcgg gaactgatta gtggatttgc cgattatgtg
     9121 ctgcaacgtg aactgggcgg caactactac agcgatcctg atgttccacc tcgcgctcat
     9181 cctgcggatg ttctgccgca agagatggat aaactgcgtg agatgatgct cgaattgatc
     9241 aaccagccgg aacactttaa gcaatggttt ggcgagttta tatcccagtc acgtcatgaa
     9301 ctggatatcg cgccgccgga accgccttat cagccagatg aaatctacga tgcgctgaaa
     9361 caaggtgatg tgctggtgcg cctgggtggt ctgcgcgtat tgcgcattgg cgacgacgtg
     9421 tatgccaatg gtgagaagat cgattccccg caccgtccgg cactggatgc actcgccagc
     9481 aacattgcgc tgactgcgga gaattttggc gatgcgctgg aagatccgtc attcctcgcg
     9541 atgctcgcgg cgctggtcaa tagcgggtat tggttcttcg aagggtaagt ttggatttaa
     9601 gccggatgcg gcgctgattg cgccttatcg tgcctacatg cactgaacgc ctgtaggcac
     9661 gataagtatg ttgaccagta tcaatataac ggtttcatcc cggtaattgg cagtccacat
     9721 acgcatcggg caatcacgac ttccgttgcg ccgttaactc ggcaatacgg acaatcacct
     9781 gcaccgcttt ttccatacct tccagagtca caaactcatg cttaccatga tagttgtaac
     9841 cgccagtgaa caggttcggg cacggtaatc ccataaacga caactgcgcg ccgtcggtac
     9901 cgccgcggat cggtttcagt tccggttcaa tatcgcagtc acgcatcgcc tgctgggcga
     9961 tatcgataat atgcggatgc tcaaccactt tctcgcgcat attgtagtaa ctgtcttcaa
    10021 tcaccagttc aatgtagcaa tcaggatgta accctttgcc cacttttttg gcgatctcca
    10081 tcattttacg tttacgcgct tcaaactgtt tacggtcgaa atcacggatg atgtagtgca
    10141 tatcggctcg gtcaacggtg cctttcatgc tcgccaggtg atagaaacct tcatagcctt
    10201 ctgtcatttc cgggctttca tccgccggaa cttccgcatg aatacgtgcc gccagcgaca
    10261 gcgcatttac catcactcct ttcgccgtgc ccggatgaac attgttaccg acaattttga
    10321 tattgaccga tgcggcgttg aagttttcaa actccagttc acctacgcca ccaccgtcaa
    10381 cagtgtaagc ccagcgggca tcgaaggcat caacatcaaa atgtttcgcc cctttgccca
    10441 cttcttcatc cggggtaaag gcgacgcgaa tatcaccatg cggaatgttt ttctgttgca
    10501 ataccgccag cgcggtcatg atttctgcaa taccggcttt gtcatcggca cctaacaagg
    10561 ttttaccatc ggtagtaatc agcgtctgac ccagtagctg atgcagcacc gggaacataa
    10621 ccggtgataa aacttcatcg ccgataccca gcgcaatatc gccaccgcga tagttttcaa
    10681 caatttgcgg attcacattt ttgccgctgc aatccggtga ggtatccaca tgagaaataa
    10741 agccaatcgc cgggatatcg ccagggacgt tagccggtaa cgtcgccatc aaagtgccct
    10801 tctcacttaa ggtcacattg ataagcccca tctcttcgag ctgctctttc agcagatgca
    10861 ataacttcca ttggccttcc gtgctgggaa cctgtctcac ccctgctttt gattgggtat
    10921 ccagagacac gtagttcaaa aatcgctcaa gtagtttatc catgtagtca ccctcacttt
    10981 ttgtgacaac attattaaga agcaagaaaa gacaaatatt gcgtcaggtc acttttaccc
    11041 ctgcaagcgg gaatatttat cagcattacc ttaatgaata taaagctaag ccagtaattc
    11101 acaacaaaga ttggcgattc aagcggtttg ccgtagaatt accgccctca ttaagagtca
    11161 ccaaggtggt taaccacaaa ccccgcatcg gtaagccatc cgttgcgttt acatgggaca
    11221 gagtaaaaaa ttgaataaac aaccgagttc gctttcaccg ctggtgcaat tggcgggaat
    11281 tcgcaaatgc tttgatggta aagaggtcat tccccagctg gatctgacta tcaacaatgg
    11341 cgagttcctc acgctgcttg gcccttctgg ctgcggtaaa acaaccgttc ttcgcctgat
    11401 tgcaggtctg gaaactgttg attccggacg catcatgctg gataacgagg acatcaccca
    11461 cgttccggcg gaaaaccgct atgtgaacac tgttttccaa agctacgcac ttttccccca
    11521 catgaccgtg ttcgaaaatg tggcctttgg gttgcgcatg caaaaaaccc ccgctgctga
    11581 aattacgccc cgcgtgatgg aagccctgcg gatggtgcaa ctggaaacct tcgctcaacg
    11641 taaaccgcat cagctctctg gtggtcaaca gcaacgcgtc gccattgctc gcgcggtggt
    11701 taacaagcct cgtctgttgt tgctggatga gtcgctctca gcgctggatt acaaactgcg
    11761 taagcaaatg cagaacgagc tgaaagcgtt acagcgtaag cttggtatta ccttcgtctt
    11821 tgtcactcac gatcaggaag aagcgctcac catgtcagac aggattgtgg tgatgcgcga
    11881 gggtcgtatt gagcaagatg gtacaccgcg cgaaatttac gaagagccga aaaacctgtt
    11941 tgttgccggc ttcattggcg aaatcaatat gtttaacgcc actgtcatcg aacgtctaga
    12001 cgagcagcgc gtacgcgcca acgttgaagg ccgcgaatgt aatatctacg ttaacttcgc
    12061 cgttgaaccg gggcaaaaac tgcatgttct gctgcgcccg gaagacttac gtgttgaaga
    12121 gattaacgac gacaaccacg ctgaagggct gattggttac gttcgtgagc gtaactacaa
    12181 aggcatgacg ctggagtcgg ttgttgaact ggaaaatggc aagatggtga tggtcagcga
    12241 attcttcaat gaagacgacc ctgactttga ccactctctc gaccaaaaaa tggccattaa
    12301 ttgggtagaa agctgggagg tcgtactggc tgatgaagaa cacaagtaag ttccagaatg
    12361 tagtgattgt cactattgtc ggttggcttg tgttgtttgt ctttctgccc aacctgatga
    12421 tcattggcac cagctttttg acccgcgacg acgccagttt cgtcaaaatg gtctttacgc
    12481 tggataacta cacgcgtctg ctcgatccgc tctattttga agtgctattg cactcgctga
    12541 atatggcgct gatcgccacc ctcgcctgcc tggtgctggg ctacccgttt gcctggtttc
    12601 tagcgaagct accacacaag gtgcgtccgc tgctgctgtt tctgctgatt gttcccttct
    12661 ggaccaactc actgattcgc atctacgggc tgaaaatttt cctcagcacc aaaggctatc
    12721 tcaacgagtt tttgctctgg ctgggcgtta tcgacacacc aattcgtatc atgttcacgc
    12781 ccagtgcggt gattatcggt ctggtttaca ttctgctacc gtttatggtg atgccgctgt
    12841 actccagtat cgaaaaactg gataagccgt tgcttgaggc ggcgcgcgat ctcggtgcca
    12901 gcaagttaca gacttttatc cgtatcatta ttccactgac gatgccggga attattgccg
    12961 gatgtctgct ggtgatgctg ccagcgatgg gcctgttcta tgtatccgac ctgatgggcg
    13021 gtgcgaaaaa cctgctgatc ggtaacgtca tcaaggtcca gttcctcaat attcgtgact
    13081 ggccgtttgg tgcagccacc agcattacac tgactatcgt aatgggcctg atgttgctgg
    13141 tttactggcg cgcttctcgt ctgctgaata aaaaggtgga actcgaatga tcggtcgact
    13201 gcttcgcggc ggttttatga ccgctatcta cgcgtacctg tatatcccaa tcattatttt
    13261 gattgtgaac tcctttaaca gctcgcgctt tggcatcaac tggcagggtt ttaccaccaa
    13321 atggtatagc ctgctgatga acaacgacag cctgttacag gcagcgcagc attcactaac
    13381 aatggcggtg ttttcggcga catttgctac gcttatcggt tcactgacgg cagttgcgct
    13441 gtaccgttat cgctttcgtg gtaagccgtt cgttagcgga atgctgtttg tggtgatgat
    13501 gtcaccagat atcgtgatgg cgatttctct gctggtgctg tttatgctgc tgggtattca
    13561 gcttggcttc tggtcgctgc tgttctcgca tatcaccttc tgcctgccgt ttgtggtggt
    13621 gacggtgtat tcgcgcctga aaggttttga cgtgcggatg ctggaagcgg cgaaagatct
    13681 cggtgccagt gaatttacca ttctgcgaaa aatcattctg ccactggcaa tgcctgcggt
    13741 ggcggcgggc tgggtgttaa gctttaccct gtcgatggac gatgtggtag tttcttcgtt
    13801 cgtcaccgga ccaagttatg aaattctgcc gttaaaaatt tattcgatgg tcaaagtcgg
    13861 cgtatcgccg gaagttaacg cgctggcaac catattactg gtgctgtcgc tggtgatggt
    13921 tattgccagc cagcttattg ctcgtgataa aacgaaaggt aacacagggg acgttaaatg
    13981 aaaaaatggt cacgccacct gctcgcggcg ggtgctctgg cactgggcat gagcgccgct
    14041 cacgccgatg acaacaacac gctgtatttc tacaactgga ccgagtacgt gccgccagga
    14101 ctgcttgaac agttcaccaa agaaaccggt attaaggtta tctattcgac ttacgagtcg
    14161 aacgaaacca tgtacgccaa gctgaaaacg tacaaagacg gtgcctatga cctggtggtt
    14221 ccttcaacct attacgtcga taaaatgcgt aaagaaggga tgatccagaa gatcgacaag
    14281 tcgaagttaa gcaatttcag caacctcgat ccagacatgc tcaacaagcc gtttgacccg
    14341 aataacgact actccattcc gtatatctgg ggggcgacgg cgattggcgt taacggtgat
    14401 gcggtagatc cgaaatccgt taccagctgg gccgatctgt ggaagcctga gtacaaaggt
    14461 agcctgctgt tgaccgacga tgcccgtgaa gtgttccaga tggcgctgcg taagctgggc
    14521 tactccggta acaccaccga tccgaaagag attgaagctg catataacga gctgaaaaaa
    14581 ctgatgccaa acgtcgcagc gtttaactcc gataacccgg ctaacccgta catggaaggc
    14641 gaagttaacc tcggcatgat ctggaacggt tctgctttcg ttgcacgcca ggcgggtacg
    14701 cctattgacg tggtgtggcc gaaagaaggc ggcattttct ggatggacag cctggcgatc
    14761 ccggcaaatg ccaaaaacaa agaaggcgcg ctgaaattga tcaacttcct gctgcgcccg
    14821 gatgtggcaa aacaggttgc cgaaactatc ggttatccaa cgccaaacct tgcggcgcgt
    14881 aagctgttaa gcccggaagt ggcgaacgat aaaacgctct acccggatgc tgaaaccatt
    14941 aaaaatggtg aatggcagaa tgacgttggc gcagccagca gcatttatga agagtattat
    15001 cagaagctga aagcaggacg ttaattcagc aagcgggcgg tggcattacc gcccgttttt
    15061 tatttagcaa ggatgtggct aatgagtcag gacagtaaag tgttatcccg cgcttttctc
    15121 ggtatcggtc tgatattgct cgttatttct gcaatcatct tttattacca ttttactttt
    15181 accaaaagcg cggttcatac agaaggaatc attgttgatg ccgtttggta caacaatcac
    15241 tcaaacgacg tcgatgataa tggttcctgg tatccagtgg ttgcctttcg ccctacgcca
    15301 gattacacgc taattttcaa ttcgaatatc ggtagtgatt tttatgaaga tagcgaaggt
    15361 gacagagtca atgtctatta tccgccaggg catccagaac aagccgaaat aaacaatccc
    15421 tgggttaatt tttttaaatg gggctttgtt ggcatagcag gcattatttt cggtagtgtc
    15481 ggtctgataa tcacgctccc ctccaccaaa aaatctcgca ggaaacggaa gtcccgccca
    15541 tgaaaaaatt cattatactg ctcagtttac tgatactcct gcctctggta gcaaccagta
    15601 aaccattgat tccaataatg aagacacttt ttacggatgt taccggtact gtaccggatg
    15661 ctgaagagat cgcacgtaaa gccgagcttt ttcgccaaca aacgggaatt gcaccgttta
    15721 ttgtgatttt acccgacatt aataatgaag ccagtctcag acaaaatggt aaagcgatgc
    15781 tggcgcatgc gtcatcttca ttgagtaatg taaaaggaag tgttctgtta ctatttacta
    15841 cccgcgaacc acggttaatt atgatcacca acggccaggt tgaaagtggt ctggacgata
    15901 aacatctcgg ccttctgata gaaaatcaca cgctggctta tttaaatgca gatctctggt
    15961 atcagggaat caataatgca ttggctgttc tacaagcaca gatattaaaa caaccgacgc
    16021 cgccactgac gtattatccg catccaggac aacagcatga aaatgctccc cccggcagca
    16081 ccaacacatt aggttttatc gcctgggctg cgacgtttat cttattttcc tggatctttt
    16141 gctacacgac tcgttttatt tatgcgttaa aatttgcagt ggcgatgact attgccaata
    16201 tgggttatca ggctttatgt ttgtatatcg ataatagctt tgcgattacc agaatatcgc
    16261 cactctgggc agggctaatt ggtgtctgta cgtttattgc cgcattgtta ttgacgagta
    16321 aacgataaaa gaggccggaa aagtgcgcgt gtattatgcc ggatggggcg cgaacgcctt
    16381 atccggccca caaaacccgc aaattcaatt aattgcgtcc ccttgtaggc ctgataagcg
    16441 tagcgcatca ggcaatgctt cctgctttat aatcccttca gcaacttttc aacaaactca
    16501 ggcaccacct ggcttgccgg gccgtaatat ttctcggcaa attcattacc gacctgactt
    16561 ggttcgagat tcagttccac ggtgtgcgcg ccatgcagtt tcgcttcgtg aacaaaccca
    16621 gccgccggat aaacatgccc ggaagtacca atggcaatga aaatatcagc catcgacaac
    16681 gccatataaa tttcatccat gccgagtggc atttcgccaa accaaacgac gtgcgggcgc
    16741 aagggtgccg ggaactggca acaatggcat ttatcttctg gcgtaacgtc tccggtccag
    16801 tcgagaacct gaccactttg cgaacaacga actttcagca attccccatg catatgaatg
    16861 acattggtat tacctgcgcg ttcatgcagg ttgtctatat tctgcgttac cagcaaaaag
    16921 cgatcgccga gggcgtcttg cagtttagcc agcgcaagat gcgcggcgtt aggctgaatt
    16981 tctggctgct gcagctgtcg acgacgggcg ttataaaacg cttgcaccag ttcaggatcg
    17041 cgatcgaaac cttccggcgt tgccacatct tcaacccgat gttcttccca caggccatct
    17101 gcggcgcgaa aggtacgaat acctgattcc gcagaaatcc ctgcccctgt cagtacgagt
    17161 actcttggtt tttccattgc ttccggcacc actttatctc tgaaaaaaat acgctgacgc
    17221 aaacgctcgc gcaggcggcg tttattttta cgaaaacgac ttaaccgatg accccgacgc
    17281 gacagcatag caacctctgt tgtttaatcg gttagatgta ggaaggccgc accacgcatt
    17341 cctcccgcat caccgtggcg cgcgcgttca atgcgcggaa cacgagctac aggtaagaga
    17401 tgacgaggca gcctgtccgc cagttgcgtt gtgattgccg ggaaattcga taagccacca
    17461 ccaatgacga ccaggtcagg gtcaacaatg gtcaggatat ttcccagaca aaccgctaat
    17521 aaatccagat aacgctcaac gtgcgccctt gcctgctcat cgccttgatc ataaagcgca
    17581 ataatttcgg gagcctgcaa cggttgatga taatagtgtt gatacagcca cgcaaaaccg
    17641 cgaccagaca gataattttc aatgcagcca tgctgaccac agccgcagcg gcgtaacggg
    17701 aaatccaacc ccatcatggt taacgcatca accggcagac gcatatggcc aaactcgccg
    17761 gtaatgtagc ttttaccggt aatcggtttg ccgttgaaaa tcagcccgcc gccaacgccg
    17821 gtgccgagaa tcaaccccat caccagtgga tattgagtaa attcatcatc ccaggcttct
    17881 gaaagggcaa aacagttggc atcgttatca aggcgtacat cgcgatcaag acgtgcgctc
    17941 aggtcggcac gcagcggttt accgctggca gcggggacat tggcggcata cagcgtacca
    18001 tcttctgttt ccggcattcc cggaataccg atgccgacag aacctttaca gccaaaacgt
    18061 tggtcagctt cagctaccag ctcacacact gcatctaaaa atgcgtcata gctgtcacgc
    18121 ggtgtcggca cccgcttttc ccactgcaac tgccgaccgc tatcaaacac gccaagcgca
    18181 atttttgttc caccaatatc aaacccgtaa tacattgccg ctccttgttt taatgtactg
    18241 cctttactgg ccgctaagga ctcgcgcagg gtcaatatta ctggcgcgcc gcgccggata
    18301 ccaacttgcc aaaagactca gcaacaatgc tgtgaccagt acgtagaaga cgtccagcca
    18361 gtgcaattcc gatggcagga aatcaataaa atagatatcg ctggagagga actgatgacc
    18421 gatcaacttt tcaatccact caataatcgg ggtaagttgc agtgaaacca ccacaccgat
    18481 aatcacccca cacaggctgc cgaacagccc tgccagcaat ccataccaga caaagatggc
    18541 gcgaattaaa ccatctttcg cccccagcgt tcttaatact gcgatatcgc cactcttgtc
    18601 tttcaccgcc atcactaagg tggagacgat gttgaaacag gccacgccaa tcaccagtac
    18661 catcgccaga tacataatgg cgcggatcat ctggatatcg cgatacatat agccgtaagt
    18721 accaatccaa cttttaatat aaacatagct gttggtcact tcacccgcat cgcgtaccag
    18781 cttattggcg ttgaaaacat ccgtcatttt aagggcaata cctgacacgc tggaacccat
    18841 atcaagatat tgttgggcat ccgccagcgg gatcatggca aaactgtgat cgagttgacc
    18901 actcaactgc aaaataccgg caacgtgcaa acgcacacgt tttggctgca tcagtttatg
    18961 ctcgggattc gagttgggga tcataatcga cacccaatcc ccctgcttca ctttcagcgc
    19021 atccgccacg cctttgccga tgataatttg ctgttcgccc gctttaaaat tgcgccacgc
    19081 atcgccctgg acaaacgagg gtaatgcgct cagacgctgt tcctgttgcg ggttaacgcc
    19141 cttcacctgg attgcgcgaa gattcacgcc actttccacc agcccggtga aattgatata
    19201 cggcgcggca gcggcaatac ccggcacttt ttgcacgtga tccagtgctt cctgccagtt
    19261 agtccacggc tgatccaccg cctcgatttc accatgcggc accaccgcca gaatacggtt
    19321 gttcagttcg cgttcaaagc cgttcatcgc gcttaagccg acgatcaata ccgccacgcc
    19381 gagggcaatg ccaatggtag aaataacgga gatcagcgac accatgccgc cgcgccgccg
    19441 tccgcgacta aaacgcaggc caattaataa cgataaaggc atcgccatta ctccgccccc
    19501 atcaggctca gttccgccgt cagacgacca tcacgcattt ctaactggcg gctcatacgt
    19561 ttcgccagtt gcaggtcgtg agtaaccacc aggaaggcgg tgccctgcaa gcgattcaat
    19621 tccccaagca actggaagat gctgtcggcg ttacgcgcat cgaggttacc ggtaggttca
    19681 tccgccagta ccaggcgcgg gttattcacc agcgcacggg caatcgccac acgctggcgt
    19741 tcgccgccag aaagttcaga tgggcggtga ttcgcacgat gatccagccc caccgctttt
    19801 aacatctcaa gtgcacggct gttgatttca gcgggctttt tcttgccaat cagcagcggc
    19861 atagccacgt tttccagggc ggtaaaatcc ggcagcaggt ggtgaaactg ataaataaag
    19921 cccagcttct ggttgcgcag ctctgctttc gccgccgaag acagtttgct catcggttga
    19981 ccgttaaaga tcacatcgcc ggaggttggc gtatccagcc cgcccaacaa gtgcagcaag
    20041 gtgcttttac cggaaccaga gctaccgacg atcgccatca tttcaccttc gccgacgctg
    20101 aaactgacat tgtgcagcac atcggtttgc acactgcctt cctgatagcg tttgcacagg
    20161 ttgtcgcatt gcaacaggat cttattcata acgtaaagcc tcagcgggtt gagtggcggc
    20221 agcgcgccat gaagggtaaa gcgtagacag cagcgcgata gccatcgcca ccagcgcaat
    20281 aacaatgacc tgtaaaggtt cgatagcgac cggcagcgcc gcgccatcaa gcaggacgcc
    20341 gattatcggc atcagattat ttaactggct ggcaagcagg gcgccaagcg ccgctccgag
    20401 gatcgcaccg ataatcccgg cgctggcccc ttgcaccata aagaccatca tgatttgtcg
    20461 cggagttaag ccttgcgttt gcaggatcgc tacttcgccc tgcttctcca ttaccatcag
    20521 ccctagtgag gtaataatgt taaacgccgc aacggcgaca atcaggctca gcagtaaacc
    20581 catcatattt ttttccatgc gtacggcctg gaacaactcg cctttacgat cacgccagtc
    20641 ctgccatttg ctgccttcag gcagtttttg ctgacttaat gagtcgactt tcagcggctc
    20701 atccagccac aaacgccagc cggtaatatt gcctgccgga taacgcatca gacgcgaggc
    20761 atcctcaata ttcaccagca tttcattgcc atcgacttca ctgttggcgg cgaaagtgcc
    20821 aatcacattg aacaggcgct gacttggaat acgccccatc ggcgtgaact ggctggcaga
    20881 tggtaccatc atgcggattt gatcaccgcg attaacgcct agctgtgagg caagttgttc
    20941 gccgaggatg acattatatt tccccggctc gaggtcagtt tgtttcacat tgaccagata
    21001 cggcgttagt ggatcttttt gcgccgggtc gataccgagc atcaccccga ccgccacgct
    21061 gcgcgcgctt tgcagtacca catcaccggt agtaataggt gcgacgcgat taacgccgtc
    21121 cagtttgact gccgtttccg ggagttgctg cgggttaaga gagccatgct cagaagagag
    21181 aattgcctgt ggcatcaggc caaggatgtt gttttgcagc tcgcgctcaa agccgttcat
    21241 cactgacaat actgtgacca gcgccatcac cccgagggta atgccgatgg tagaaagcca
    21301 ggagacgaaa cgaccgaagc gatccgctgc acgcccacgc atgtaacgca ggccaataaa
    21361 tagagcgaca ggttggtaca tgaaatccgt ctggttgctg tagcaaagac acgagtatat
    21421 aagcgaaagc aagatgggta aacgtctgaa ctgtaagtga tcgtcagggc taatctgaaa
    21481 agcccccgtg aatcaatcgg ctaaattgcc atgttttatc atcaatcttg ccgcctgatc
    21541 ttaatctgaa aaatcatcca atacagacag atacccatta cacatagggt cataacccgc
    21601 aggatcaacc gcatgtaaat taaaaaagag cgtgttgtcc tgatgaaaca aaaagagcta
    21661 tggattaacc agatcaaagg gttatgtatt tgtctggtgg tgatttatca ctcggtcatt
    21721 accttttatc cgcatctgac cacttttcag catccgttat cggaagtcct gagcaaatgc
    21781 tggatctatt tcaatcttta ccttgcaccc tttcgtatgc cggttttttt ctttatttcc
    21841 ggctatttga ttcgccgcta tatcgacagc gtaccgtggg gaaattgtct cgataaacgc
    21901 atctggagca tcttttgggt gctgacgctt tggggcgtgg tgcagtggct ggcgttaagt
    21961 gcgcttaacc agtggctggc acctgagcgc gatttaagca acgcttccaa tgccgcgtat
    22021 gccgattcca ccggtgagtt cctgcacggg atgatcacag ccagcaccag cttgtggtat
    22081 ctgtatgcgt taattgtcta tttcgtggta tgtaaaattt ttagccgtct ggcgctgcca
    22141 ctattcgcct tgtttgtact gctgagtgtg gcggttaatt tcgttcccac gccgtggtgg
    22201 ggaatgaaca gtgtgatccg caatttgcct tattacagcc ttggcgcatg gtttggcgca
    22261 acattaatga cctgtgttaa agaggtgccg ttgcgccgcc atctgctgat ggcttctttg
    22321 cttaccgttc tggcggtcgg tgcctggttg tttactatct cgctgctgtt gtcgctggta
    22381 tcgattgtgg tgatcatgaa gctgttttat caatacgagc aacaattcgg tatgcgctcc
    22441 accagcctgc tgaatgtgat tggctccaac accattgcta tctacaccac ccatcgcatt
    22501 ctggttgaaa tattcagctt aactctgctt gcgcaaatga acgcagcacg ctggtcgccg
    22561 caagtcgaac tgacactcct gctgggttac ccctttgtta gtttgttcat ctgtactgtt
    22621 gcgggcttgc tggtaagaaa actttcacag cgcgcattca gcgatctgtt gttctccccg
    22681 ccttctctgc ccgcggccat cagttactcc cgctaagcca tcgccccagc cggggcgatt
    22741 caatttgcta aaccatgtcg attacggata atgaacagca ttgcttatca gagatatgcc
    22801 cccatatgtt gaggcatatc ctaacgagaa tctgacaacc gttatgcctg aacaatatcg
    22861 ttatacgctg cccgtcaaag cgggtgagca gcgtctgctg ggcgagttaa ccggcgctgc
    22921 ctgcgcgacg ctggtagcgg aaattgccga acgtcacgcc ggtccggtgg tactcattgc
    22981 accagatatg caaaatgctc tgcgtttgca tgatgaaatc agccagttta ccgatcaaat
    23041 ggtgatgaat ctggcggact gggaaactct tccctacgac agtttttcgc ctcatcagga
    23101 cattatctcc tcgcgccttt ccacccttta ccagctaccg acgatgcagc gtggcgtact
    23161 gattgttccg gtgaatacgc ttatgcagcg cgtttgcccg cacagttttc ttcacggtca
    23221 tgcgctggtg atgaaaaaag gtcagcgtct gtcacgagat gcattaagaa cccaactgga
    23281 cagcgccggt tatcgccatg ttgaccaggt aatggagcac ggcgaatacg ccacgcgcgg
    23341 cgcgttgctg gatctcttcc cgatggggag tgagctgcct tatcgtcttg atttctttga
    23401 tgatgaaatc gacagcctgc gggtgtttga cgtcgacagc cagcgcacgc tggaggaagt
    23461 agaagcgatc aatctgctgc ccgcgcacga atttccgacc gataaagcgg caattgaact
    23521 gttccgcagc cagtggcgcg ataccttcga agtgaagcgc gatccggaac atatttacca
    23581 gcaagtgagt aaaggcacat tacctgccgg gatcgagtac tggcagccat tgttcttcag
    23641 cgaaccactg ccgccgctgt tcagttattt ccctgccaat accttgctgg tgaatactgg
    23701 cgatctggaa accagtgccg aacgtttcca ggctgacacg ctggcgcgtt ttgagaatcg
    23761 cggcgtcgat ccgatgcgcc cgctgttgcc accacaatcg ctctggctgc gggtggacaa
    23821 gctcttctca gagctgaaaa actggccccg ggtgcagcta aaaactgaac atttaccgac
    23881 aaaagccgcg aatgccaatt taggtttcca gaaactgcca gacctggccg ttcaggcaca
    23941 acaaaaagcg ccgctggatg cgctgcgtaa gttcctcgag actttcgacg gtccggtggt
    24001 gttctcggta gaaagtgaag gtcgccgtga agcgctgggt gaactgctcg cacgaattaa
    24061 aattgctccg caacgcatta tgcgtcttga tgaagccagc gaccgtgggc gttatctgat
    24121 gattggcgct gccgaacatg gttttgtcga taaggtgcgt aatctggcgc tgatctgcga
    24181 aagcgatctg ctcggtgaac gtgttgcccg ccgtcgtcag gattctcgcc gcaccatcaa
    24241 ccccgataca ctgatccgta accttgcgga gctgcatatt ggtcagccgg tggtccatct
    24301 ggagcacggt gtcgggcgct atgccggaat gaccacgctc gaagcgggcg gcattactgg
    24361 cgagtatttg atgctcacct atgccaacga cgccaaactg tatgttccgg tgtcgtcact
    24421 gcatctgatt agccgttacg caggtggcgc ggaagaaaac gccccgctgc ataaacttgg
    24481 cggcgatgcg tggtcacgcg cgcggcagaa agcggcggaa aaagtgcgtg atgtggcggc
    24541 ggaattgctg gatatctacg cgcaacgcgc cgccaaagag ggcttcgcgt ttaaacacga
    24601 tcgtgagcag tatcagttgt tctgcgacag cttcccgttt gaaaccacgc cggatcaggc
    24661 gcaggccatt aatgcggtac ttagcgacat gtgtcagccg ctggcaatgg atcgtctggt
    24721 gtgcggcgat gttggctttg gtaaaacaga agtggcgatg cgcgccgctt tcctggcagt
    24781 agataaccac aagcaggtgg cggtgttggt gcctaccacc cttctcgcgc agcagcatta
    24841 cgacaacttc cgcgaccgtt tcgccaactg gccggtacgt atcgaaatga tctcccgttt
    24901 ccgcagcgcc aaagagcaga cgcaaatcct tgcggaagtg gcggaaggga aaatcgatat
    24961 tctgatcggt acgcacaaac tgctgcaaag tgacgtcaag tttaaagatt taggcctgct
    25021 gattgtcgat gaagaacacc gcttcggggt gcgtcataaa gagcgtatta aagcgatgcg
    25081 cgcgaacgtg gatattctga cgcttactgc aacgccgatc ccacgtacgc tgaatatggc
    25141 aatgagcgga atgcgtgacc tgtcgattat cgccacgccg cccgcccgtc gtctggcagt
    25201 taaaaccttt gtccgtgagt atgacagcct ggtggtccgg gaggcgatcc tgcgtgaaat
    25261 tttgcgcgga ggacaggttt attatctcta caatgatgtg gaaaacattc agaaagccgc
    25321 cgaacggctg gcagaactgg tgccagaagc gcggatcgcc atcggtcacg ggcagatgcg
    25381 cgagcgcgaa ctggaacggg tgatgaatga tttccatcat caacgtttca acgtgctggt
    25441 ttgtacaacc attatcgaaa ccgggatcga catcccgaca gccaacacca ttatcattga
    25501 acgcgcggat cacttcggtc tggcgcagct gcaccagtta cgcggtcgcg tcggacgttc
    25561 acatcatcag gcatatgcat ggttgctgac gccgcatcca aaagcgatga ctaccgatgc
    25621 acaaaaacgt cttgaagcga ttgcctcgct ggaagatctc ggggcaggtt ttgcgctggc
    25681 aacgcacgat ctggagattc gcggcgcggg tgaactgctt ggcgaagaac aaagcggctc
    25741 aatggaaacc atcggtttct cgctgtatat ggagttgctg gaaaacgccg tcgatgcact
    25801 gaaagccgga cgcgagccgt cgctggaaga tctcaccagc cagcaaacag aagtcgagct
    25861 gcggatgccg tcgctattgc cagatgattt cattcctgac gtgaatacgc gtttgtcgtt
    25921 ctataaacgt attgccagcg ccaaaacgga aaacgaactg gaagagatca aagtcgagct
    25981 tatcgatcgc ttcggcctgc tgccggatcc ggcgcgtacc ctgctggatg ttgcccgtct
    26041 gcgccagcaa gcgcagaaac tggggatcag gaagctggaa ggtaatgaga aaggcggggt
    26101 gatcgaattt gccgagaaga atcacgttaa tccggcctgg ttgattggtt tgctgcaaaa
    26161 acagccgcag cattaccgcc ttgatggtcc gacgcgcctg aaatttattc aggatttgag
    26221 tgagcggaaa acgcgtatcg aatgggtacg ccagtttatg cgtgaactgg aagagaacgc
    26281 gatcgcttaa tggtaaatcg ggggcgtttc tgcgccccca tctgcaacat ttacaaattc
    26341 tttgcacttc cctgcactat ccgacactgt caccatccat aattcaggct tatctgttta
    26401 ttacaataac cttatattta ttatggattt ttggtgatga tcaaaacgcg tttttctcgc
    26461 tggctaacgt tttttacgtt cgccgctgcc gtggcgctgg cgctaccggc aaaagccaac
    26521 acctggccgc tgccgccagc gggcagtcgt ctggttggcg aaaacaaatt tcatgtggtg
    26581 gaaaatgacg gtggttctct ggaagccatc gccaaaaaat ataacgtcgg ctttctcgct
    26641 ctgttacagg ctaaccctgg cgttgatcct tacgtaccgc gcgcgggtag cgtgttaacg
    26701 atcccgttgc aaaccctact tccagatgcg ccgcgcgaag gcattgtgat caatattgcg
    26761 gagctgcgtc tctattacta cccgccgggt aaaaattcgg taaccgtgta tccaataggt
    26821 attggtcagt taggtggtga cacgctgaca ccgacaatgg tgaccaccgt ttcagacaaa
    26881 cgtgcaaacc caacctggac gccaacggca aacatccgcg cccgttataa agcacaggga
    26941 attgagttgc ctgcggtagt gccggctgga ccggataacc caatgggcca tcatgcgatt
    27001 cgtctggcgg cctatggcgg cgtttatttg cttcacggta cgaacgccga tttcggcatt
    27061 ggcatgcggg taagttctgg ctgtattcgt ctgcgggatg acgatatcaa aacactcttt
    27121 agccaggtca ccccaggcac caaagtgaat atcatcaaca ctccgataaa agtctctgcc
    27181 gaaccaaacg gtgcgcgtct ggttgaagta catcagccgc tgtcagagaa gattgatgac
    27241 gatccgcagc tgctgccaat tacgctgaat agcgcaatgc aatcatttaa agatgcagca
    27301 caaactgacg ctgaagtgat gcaacatgtg atggatgtcc gttccgggat gccggtggat
    27361 gtccgccgtc atcaagtgag cccacaaact ctgtaaatcc tggataaaaa aaatccccgc
    27421 gacacggcat gtcgcgggga aaaaatcagc agtagcaggc attaatgagg gttaatgctt
    27481 atttataaat tactgctgtt ccatggaggg tattcggacc ggttacagaa gtaatacgga
    27541 aagattttgc gcccatctca tccgcttttt gcgccagctg ctcttccagc gatcccagat
    27601 ttgtccccgc gttagcactg attgtaccga ctttttgttg gccttctggc gttgactgaa
    27661 cttcgacagc cgcaaagctg gcaaatgaca tggagcttaa aatcgccgca gcgatgaggg
    27721 tttttacgtt tttcataata gtggccttat gcagatgaat gacgtcgcaa gcaattactt
    27781 aacgatcgat aactaaatga tagatgtgat ctggatcaca tacaagatat tttttataac
    27841 aatcattaat taatttaaaa atctttaaat ttcagttaaa tagaaagcaa ttatttttat
    27901 gggatctgga ctggtgaacg gaggtgatta tttttataat aacctttcat tcacagtcac
    27961 caggtacaac ggcatcatgg caactgactc aacacaatgt gtaaaaaaaa gccgtggccg
    28021 cccaaaagtg ttcgacaggg atgccgcgct tgataaggcc atgaaattgt tctggcaaca
    28081 cggttatgaa gcgacttctc ttgcggacct cgtcgaagcg accggagcca aagcgcccac
    28141 gctatacgcg gaatttacca acaaagaggg gttatttcgt gccgttctcg accgctatat
    28201 cgatcgtttt gccgctaagc atgaagcaca gctgttttgt gaagagaaaa gcgtggagtc
    28261 tgcgctggct gactattttg ctgccatcgc caactgcttt accagcaaag acaccccggc
    28321 aggctgcttc atgatcaaca actgcaccac cctctcccca gattcaggag atatcgccaa
    28381 tacgttgaaa tcacgccatg cgatgcaaga gcgcactttg cagcagtttt tatgtcaacg
    28441 acaagcgcgc ggggaaatcc cgacccactg tgacgtgaca catctggcag aattccttaa
    28501 ttgtattatt caggggatgt cgatcagcgc acgcgaaggt gcatcgctgg aaaaactaat
    28561 gcagattgcc ggaacgactt tgcgtttatg gcccgaactg gtgaaataag tcgcaaaaaa
    28621 agcccctcag cctgagcgaa tgaggggcca aattgcagag tacagccttg ttatactttg
    28681 ttattcaaaa tcagttgccc attactatct agcgggatct gcgtacccgg atcgcggtcc
    28741 atgcggattt tgccctgctg atcgccaatc ttataggtca catcataacc gagcattttt
    28801 tctgacttgt catacaccgt tttacaacgc tgttgcgtag tcgtgtaagt atcgctttcc
    28861 tggagagagc cctggatctg gttaccggca tatccaccac ccagcgcccc cacaacagtg
    28921 gcgacatctt taccgcgacc accgccaaac tgatgcccta tcacgccgcc agcaacagcg
    28981 ccgagcaccg acccggtaat gcgattttca tcctgcaccg gtcgacgatg ggtcactgtg
    29041 acgttgcgac actcctgacg cggtgtttta accgtttcct tgattggggt tgcagaaaca
    29101 acctgagcgt attgcgggcc acgttcaaac acgttcagac tggccactgc cgctacgccc
    29161 agcgcagctg cgacaccaat cccgataccc gccaacattg atttattcac gggaacacct
    29221 ccttctttgc agttatgcca atcatgcaag cgaaggagaa aagcagctat cagacagaag
    29281 atgaaaagcg cgggaatggc tggaaatact taaggattcg gaggagtctg aaggaatgcc
    29341 tgatgcgacg cggctgccga tcgcgctgtt cctcaagggc ggataaggcg tttatgccac
    29401 atccgccagt gtacgtcgat taatgcaact tcaaacgcgg acggataacg cggttaatac
    29461 tccccaccag catcattaat ccggttttaa agtaaccatg cagcgcaatc tgatgcattc
    29521 ggtatagcga gatatataca aagcgcgcaa ttcgtccttc aatcatcatt gagccgcgcg
    29581 tcaggttacc catcaggcta ccaacggtgg agaagttcga cagcgatacc agcgaaccat
    29641 gatctttata ctgataattt ttcagcggct taccattcat ctgcgccaga atgttgttca
    29701 ttgcgcaagt cgccatctgg tgtgcagcct gagcacgcgg cggaacaaag cccccttccg
    29761 gacgcgggca tgacgcgcag tcgccaatag cgtaaatgtc tggatcgcgg gtggtttgca
    29821 gcgtcggttc caccaccagc tggttgatac ggttagtttc aagaccaccg atatctttca
    29881 ggaagtctgg cgctttgatc ccggctgccc acaccatcag atcagcctca atatattcgc
    29941 catctttagt gtgcaggccg ccttcatcag cactggtgac catggtttgc gtcagcacgc
    30001 gaacgccaag tttcgttagc tcgttgtggg ccgcagcaga gatacgtggc ggtaatgcag
    30061 gcaaaatacg ttctcccgct tctaccagcg ttacgttcag ggcttcgttg gtcaggcctt
    30121 tgtaaccgta gctgtgcagt tgcttgaccg cgttgtgcaa ttcagcggag agttctacac
    30181 ccgtcgcgcc gccgccgaca atcgcaatgt tcactttgcc atttgcgccc aggttggcgg
    30241 agtatttcag gaagagattc agcatctcct ggtgaaagcg acgcgcctgg tgcgggttat
    30301 cgaggaaaat gcagttctct ttgacgcctg gcgtattgaa atcgttagag gtgctaccca
    30361 gcgccatgac cagggtgtca taggcgattt tacgttccgg aaccagcagt tcacctttct
    30421 cgtcgcgtag ttctgcaata gtgattgttt tcgcttcacg atcaatatca atgacggaac
    30481 ccaactggaa ctggaaacca tgattgcgcg catgggccag atagctcaac gcatcgacgc
    30541 cttcatcaag cgagccagtc gccacttcgt gcagcagcgg tttccacagg tggctgtggt
    30601 tacgatcgac cagcgtaatt ttggcttttt tcttacgtcc cagcttatgc cccagctgtg
    30661 ttgccatttc cagcccacca gcaccgccgc cgacaatcac aatctttttc aatggcgtag
    30721 tcaacgtgac ccccttaaat ttattaacca attgttaatt aatggttatt aacatagctc
    30781 ttaattaaca acaggttaca gcaatgaaaa tgttcttcgc tattgagaat aacatgaatg
    30841 gcgcattggt cataccaaaa ttgatgttaa tcaagttttg ttgctgaaaa gataggcgac
    30901 tttaccgggt gagatcaaaa aatagagttt attgtttatt atttgagcaa attcggaaga
    30961 gtaatatagg gtgaagcgta tatcgagggc gtttggtcgt cctgataaga tgcgcagcat
    31021 cgcatcaggc attgatgctc gactgtcgga tgcggcgtgg acgccttatc cgacttacag
    31081 aattcggagc gcatcaggta ccggtaaggt tacctgatgc gacgagggca tttacccgag
    31141 ggttttgaac gctttaatgc gctgtaaatg cggggagata ttcttgaatt tgtgcgtctg
    31201 ctcttcgtcc cagacaatct cgtaataatg atgcaactct tcagatgtcc gctggctgtt
    31261 aagtgcttca tcattacgcg acaaaataac cagacaacga tcgcgattct tctcgcggaa
    31321 gttggtcaca cacttagtgg caatatcggc atactcttcc gggcgatcaa tcttgccttc
    31381 catgttctca taagggaaca aattagggtt gaagatcacc tggcggatat cgcaaagaaa
    31441 accaatccgt tccgcccagt atccgcccag gccaacgccg caaattagcg gacgctcgtc
    31501 aacgttcagt tgcaacattt tgtccacttc tttaagcaga tgctgcatat catgtttcgg
    31561 atgccgcgta ctgtagctta tcaagcgtac atccgggtca ataaactgca attgtaagac
    31621 tttctcgtgg ttacccggac tgttagagtc aaaaccgtgt aaatagataa tcatcgcttc
    31681 ctcacataag ccagggttag tgacctgctt tctcttcctg ccagcgttcg tgtaactgat
    31741 tcagacgggt gctgatcgct ttccagcgag ccgagtccat cagttcctgt cgcgaaaatg
    31801 aacctttatg atacaaacgt gtaacacgtt ctgccttgat cggtgacaga ttatctaaca
    31861 cgctgacggc ccctttacga ttattgcaga ccaggatcat atcgcaaccc gcatccagtg
    31921 atgcctgccc gcgttcggca taactgccca taatcgcggc accttccatc gataaatcgt
    31981 cagagaaaat cacgccgtca aaacccagtt cctgacgcaa aacggttttc agccagtagg
    32041 gagaaccgct cgccggacgc ggatcaacat cactgtagat cacatgcgca ggcataatgg
    32101 cgtcgagttt attttcgcgg attaaggaac tgaagaccga catatcttta gcgcgaatct
    32161 ccgcttgtgg acgtggatcg cacggtgttt ctttgtgtga gtctgccgtt actgcaccgt
    32221 gtcctgggaa gtgtttcccg gtcgttttca ttccggcttc atgcatacca tcaataaacc
    32281 ggctggcaat tgccagggct ttttgtggat cggcatgata agaacgctcg ccaatcgccg
    32341 cgctgatatg cccgacatcc agcactggcg caaagctgat atcaatatcc atagcgatca
    32401 tttcgctggc catcagccaa cccgcctctt gcgccagttt gccgccctct tccattcctg
    32461 acagcgcagc gaatgattgc gccgctggca agcgggtaaa accttcacga aaacgctgca
    32521 cgcgtccacc ttcctgatct accgccacca ccagatgatt gcgcgaagct gcgcggatct
    32581 ggcgcaccag ttcacgtaac tgggcaggat catgatagtt acgcgtaaag agaatcagcc
    32641 ctcccaccag cggatgcgca agtatttcac gctcttccgc gtccagttcg taacctttga
    32701 catccaacat tactggaccc acactgctct ccttattgtt ttattaatag ctgccgccag
    32761 gtgtcatcgg ccagcctgat aaattgttga tcgccggttt gtcgccagcg gtactcaaac
    32821 caccctgctt tgagcatcag cagccaggga aaccatcgcc tgacctgacg ccataattgc
    32881 gccggataaa tcttcgcgcg agtggcatag tcattgacca attgccggtg ctgttcagta
    32941 ttttccaccc acaccgccgc cagttccagc gcgatatcac catctccggc atactcccag
    33001 tcgatgagtt ttaaccctga cgcgctatgc actaaatttc cggcgtggac atccatatgc
    33061 aatggactta agcgtaaagg ccgtggttcc cgcgctttgc gcagacgttt taacattcgc
    33121 agccaaccca ctgtccgccg cgccggatcg ctttgctgcc agtacagttc cagtaacggc
    33181 aacagcgtta ttcgccagcc aaaacgtggt tgttgatgta gataatacag caagcctgcc
    33241 agttcgttgg tatccggcaa atacgttttt accgcgccgg gcagatagtc gactaccatc
    33301 cagtcacgga gatataaatg cggcttcggt gcaatgcttg cgggtagttg tgataaagcc
    33361 cgatactggc gcaagaacgc ggactgcggc gcatcaggat cgtgcggctg acgcacaaca
    33421 aaacgctggc cctgatgttc aatgagaaaa ctcccgccac taagcccact attaaacgtc
    33481 gtgacgggat gaaactgcgg gaaaaagcgc gacagcaatt cgtcgcgcat gatgggatta
    33541 ttgctgcgaa acggcacctt tacctgacca gataatttcg cccgtctgca ccagcatcag
    33601 ctgcatttgt agggtcggag cgttaacgtt gccagaggcg ctggagtaca gcacgtaatg
    33661 agcgccgaca ttgcgggcaa tgcctatggc tttactacgg gtacctaaac tgtcctgcgg
    33721 cgacaaacct aactgttgct tagccatcga cagctgctgg gcggaaacca gggtaaattt
    33781 cccgttatta gccagcgcat ttcgcagcgt ttcggtcgct tctgcggcat tcagcgaacc
    33841 gttagtacgg ttgttaacgc tatcaaccag caggacgcta cccgcagtca ccccgtcagc
    33901 cccaagcatc ttactgacca tcggctgcat tgcgccattc cagtcataat ggcgaatatg
    33961 cggcgcaggc ggtgccgttt gatcttcgtg ctcaattggg cctggctgct gcgggatcgt
    34021 cggcaccgag ggcactgtgg ggacaggctg ttgtggctcg gctggttgtt ccggcgctgg
    34081 tttcacttct tctaccggtg caggttcacg ttgccccaca cacccggcga gaaacatcgc
    34141 cagcgcggta atcaaggcgt agcgactcat ttttgtcatc aagattcacc ccttacaaat
    34201 atagataaag tctgactttg tgcgccccca gaaaattggc gctgccgtac agcgttaccg
    34261 ccgaatgtgc gggaatgcta acgctgcgtg gcctttccag aggatgcatc tccagccctc
    34321 tggcgtcata ccagtaaaaa cgataatgaa cggtaacggg ttcttgcctt tcgttataaa
    34381 gcgttgagga tgctgaaggt tgaatatcag acgtcgaaag gacgggcttt tctgcactga
    34441 tgcccgcagc caataaagat gactccatca ccagcgattg ctcatcattc accggaattt
    34501 ccggatgtga acgacagccc accagcagta acaacgccag agacaccagc ccaaagcatc
    34561 cttttctcat cgttacagac ctttatgcgc cagcattggt cccagcggac ggccacccaa
    34621 caagtgcata tggatgtggt aaacctcttg tccgccatgg cggttggtat tcatgatcag
    34681 acgatagcca tcttcggcaa taccttcttg ctcagcaatt tttgccgcta cggtgatcat
    34741 gcgtcccagc gcctgctcat gctcagctga cacgtcgttc acagtcggaa tgaggatatt
    34801 cggaatgatc agaatatgcg ttggcgcttg cggcgaaata tcgcgaaacg ccgttaccag
    34861 atcatcctgg tagacgatat cggaggggat ctcacgacga ataattttgc tgaatatagt
    34921 ttcttctgcc acgacatttt cctttttcaa atagccatgc gttgcgccat gctacgccgc
    34981 gccacactgc gagtatagag tatggtcgag ttaaacctgc tctttcaacc ttaactcacg
    35041 atttcttaag caaaaaatcg ttttgctgcc gtatatatcg ccattattcc catttctgcc
    35101 tgcgaatgcg ctgaaaagac attctctgtt tggtttacac gacaaatatg aatgcgtata
    35161 tttctcattt gcatttacaa acaaaattat tcgcacacta aatataaccg ctcatcaccc
    35221 tcctccggca tgagcctgac gacaacataa accaagagat ttcaaatact ttcaacacaa
    35281 tttaacaggg ataatcaata tcaagccagc accaaaccat cactacttgc cggttgcata
    35341 gcattggcac tattaccttc tgccgctttt gccgctccag ccactgaaga aacggtgatt
    35401 gttgagggtt cagccacagc tccagatgat ggcgaaaatg attacagcgt aacgtctacc
    35461 tctgcgggta ccaaaatgca gatgactcaa cgtgatattc ctcagtcggt cactattgtt
    35521 agccagcagc ggatggaaga tcagcagtta caaacgctgg gcgaagtgat ggaaaacacg
    35581 ctggggatca gcaaaagtca ggcggattcc gatcgtgctc tttattattc ccgcggattc
    35641 cagatcgata actatatggt tgatggtatc cccacctatt ttgaatcgcg ctggaatctg
    35701 ggcgacgcac tttctgatat ggcactcttt gaacgcgtag aagtagtgcg tggcgcgaca
    35761 ggactcatga ccgggacggg taatccatct gcggcaatta atatggttcg aaaacacgcg
    35821 accagtcgtg aatttaaagg cgatgtctcg gcggaatacg gtagctggaa caaagaacgg
    35881 tatgtggcgg atttacaaag cccactcacc gaagacggta aaatccgcgc gcgaattgtc
    35941 ggcggctacc agaataacga ctcatggctg gaccgctaca acagtgaaaa gaccttcttc
    36001 tcgggcattg tcgatgctga tttaggcgat cttacgacgc tttcagccgg ttacgaatat
    36061 cagcgcattg atgttaatag ccctacctgg ggcggtttac cgcgctggaa tactgatggc
    36121 agcagcaaca gttacgatcg tgcacgcagt accgcacctg actgggcgta caacgataaa
    36181 gagatcaaca aggtctttat gaccctgaag cagcggtttg ctgatacctg gcaagcgaca
    36241 ctgaatgcca cccactctga agtcgaattt gacagcaaaa tgatgtatgt cgatgcctat
    36301 gtaaacaaag cggatggtat gctggttggg ccatacagta attatggacc tggctttgat
    36361 tatgtcggcg gcaccggttg gaacagtggc aaacgtaaag ttgatgcgct ggatttgttc
    36421 gctgacggta tttatgaatt gtttggtcgt cagcacaatc taatgtttgg tggcagttac
    36481 agcaaacaaa acaatcgtta cttcagttca tgggccaaca tcttcccgga tgaaattggc
    36541 agtttctaca actttaatgg caatttccca caaaccgact ggtcaccaca gagcctggcg
    36601 caggacgata ccacacatat gaaatcgtta tatgctgcca ctcgagtcac ccttgccgat
    36661 ccgctgcatc tgatcctcgg cgcacgttat accaactggc gggttgatac gctgacttac
    36721 agcatggaga aaaaccacac cacgccttac gctggtctgg tgtttgacat caatgacaac
    36781 tggtcgacct acgccagcta tacctctatt ttccagccgc aaaatgatcg tgacagttca
    36841 ggcaaatatc tggctccaat caccggtaac aactacgagc tgggtctgaa atcggactgg
    36901 atgaatagcc gtctgaccac cacgttagcc atcttccgta ttgagcagga taatgtcgct
    36961 cagtccaccg gtacacctat ccccggcagc aacggcgaaa ccgcctataa agcggtggat
    37021 gggacagtca gtaaaggggt ggaatttgaa ctcaacggcg caattaccga caactggcag
    37081 ctgacatttg gcgcaacgcg ctatattgca gaggataacg aaggaaacgc cgttaatcct
    37141 aatctgccac gcaccacggt taaaatgttc accagctatc ggttgcctgt catgccagag
    37201 ttgacagtcg gcggtggtgt taactggcaa aatcgcgtgt ataccgacac cgtgacaccg
    37261 tatggcacct tccgcgccga gcaaggtagc tacgcgctgg tggatctctt cacccgctac
    37321 caggtgacga aaaacttctc gttacagggg aacgtcaata acctgttcga caaaacctac
    37381 gataccaacg tggaaggttc tatcgtctac ggcacaccgc gtaatttcag cattaccggc
    37441 acgtatcaat tctgaataac acctgtaaaa aaggcagcca tctggctgcc ttagtctccc
    37501 caacgtctta cggattagtg gttacggatg tactcatcca tctcggtttt caggttatcg
    37561 gatttagtac cgaaaatcgc ctgaacacca gaaccagcaa ccactacgcc cgctgcgccc
    37621 agtttcttca ggccagcctg atccacttta gacacatcag caacgctgac gcgcagacgg
    37681 gtaatacatg cgtcgaggtt agtaatgttt tctttaccac caaatgcagc aaccagagcc
    37741 ggtgccattt cgctggtacc tgtcgctttt gcatcttcag tcgcgtcttc acgacccggc
    37801 gttttcagat ccagtgcttt aatcagcacg cggaagatgg tgtagtaaac aatcgcataa
    37861 ccgataccga cgatcgggaa cagccacagt ttgctgctgt taccagacag aacgatgaag
    37921 tcgatcagac cgtgcgagaa cgacgtaccg tcacgcatcc ccagaagaat acagattggg
    37981 aatgccaggc ctgccagaat cgcgtggatg atgtacagga tcggcgcaac gaacatgaag
    38041 gagaactcga tcggctcggt gataccggtc aggaacgagg tcagcgccgc ggagatcata
    38101 ataccgccca ctttcgcgcg gttttctggt ttagcagagt gccagatagc aattgcggca
    38161 gctggcagac cgtacatttt gaacaggaag ccaccagaca gtttacccgc agtcgggtca
    38221 cccgccatat aacgcggaat gtcgccgtgg aaaacctgac ctgctgcgtt ggtgtattca
    38281 ccaatctgca tctggaaagg tacgttccag atgtggtgca gaccaaacgg taccaggcaa
    38341 cgttcgatga aaccgtaaat gccaaacgca actaccgggt tctggtaagc agcccactga
    38401 gagaaggtct ggattgcaga accaatcggc ggccaaatga aggacagcac aacgccagta
    38461 aagatggcag ccaggccaga aatgatcggc acaaagcgtt taccggcaaa gaagccaaga
    38521 tactcaggca gcttaatacg gtagaaacgg ttaaacatgt acgctgcgat cgcaccggag
    38581 ataatccctc cgagtacgcc agtatccgcc aggtgtttag aggcgatttc ttcagcaggt
    38641 aaatgcagta ccagtggcgc aaccacggcc atggttttaa ccatgatgcc ataggcaaca
    38701 actgcggcca gcgcggatac gccatcgtta ttggtaaagc cgagggcgac accgatcgca
    38761 aaaatcagtg gcatgtttgc aaagacggaa ccgcctgctt ctgccataac atgcgataca
    38821 acggcgggca gccagctgaa attcgcggaa ccgacgccca gcaggatacc tgcgataggc
    38881 agtacggata ccggcagcat cagcgattta ccgacctttt gcaggttagc aaatgcattc
    38941 ttaaacataa ttgagagtgc tcctgagtat gggtgctttt tttacgttct cacgcgtggc
    39001 aaggggggag agcctcgccg tgtacagggc atctaagcgc cctttattta ttacacagag
    39061 taaaataatt tagtgccaat ttgtttgacg gctatcacgt ttcaacttca gatcattgga
    39121 gaagttgcac attttgacaa aacatatgtc acaatatttc aaacgtggtt attcaccgcc
    39181 caattttcgg cggtgaactt tactcgtttt agccattaat tacgagcttt aaaaaaactc
    39241 attcaacgga tggattgaag gcgggaagcg tcgatgtgaa acagacgggc gaagttatcg
    39301 gtggttacct gcgccagttc ttcaacggca acacctttca acacagccat gtattctgca
    39361 acgtcacgaa ccatcgcagg ttgattctct tttcctcgat gcggtaccgg cgcaaggtaa
    39421 ggtgagtcag tttccaccag taatcgatcc agggggacat aacgcgcagc atcgcgcagt
    39481 tgctccgcat tacggaaggt cacaatgccg gaaaaggaga tgtaaaatcc gagatccagt
    39541 aatttacccg ccgtttctct gtcctctgta aaacagtgta gtacgccacc gcaatccgtc
    39601 actttttctt cgcgcagaat tgccagcgta tcggcacggg cgtcacgggt atggacgata
    39661 accggcttgt tcagttcacg accaatctgg atatgatgga tgaaggactc ttgctgacgt
    39721 actttagttt ccggtgtgta ataataatcc agcccggttt cacccagcgc tacaacaccc
    39781 tcttctgccg ccagacggcg taaatcttct acatcgtagg gatcattctg gttgagtgga
    39841 tgcacgccac aagaaaatac gacgttgtca cgttcgccta ccagatcccg catatgtaaa
    39901 taacccggta atgttgtggc gactgccaga caaaatttca catcgcgtgc ggcggctttc
    39961 gccagaacgt catccacgtc cttatgcaaa gattcataat ccagaccatc gagatggcag
    40021 tgtgagtcga ctaaaaacat gatgtctctc ttaaagatga ggaaccggta gcacaacgcc
    40081 cggttgcagg taatgctcaa tacgcagtaa aagatcggtg atgagaagct cgcggttgat
    40141 gcctgtaaca gacattaact gttcacgaat gtggcaaaca tcccccagta tagcctgcag
    40201 gcgcgaggga gaaagatggt ttgccagttc ggcgaccagg cccggcacat caacattggt
    40261 cacctgcgca gcaccatgat ggcgttttag cgcatccatc agcaacgttg ccagccagtg
    40321 taaacgcgcc ggagcttgtt catgattaag ggccgctagc agcgaatacc agtcgcccga
    40381 tggcacgcta tatgccaacg cctgacacaa tgtttcacga gcctgccagt tatctccctg
    40441 aaacaacgcc agtgccgcgc caggcgaacc ggcgcttaag cgcaatgcgg caagtaatgc
    40501 atcctgtgac attgtcactt cgcgtgaaag ccaggtcacg gcgtactgtt ccggcggcgg
    40561 cgcaaggtaa tgtaaccgac aacgactacg taatgttgcc agtaaacgtt caggctcgcg
    40621 ggtagccagg aaaaaccagg tttctgctgg tggctcttca agcgttttca gcaatgcgtt
    40681 agccgcggcg tcggtcagta aggcagcatc ggttacccag acaactttcg caccacctag
    40741 gcgtgcgtgc tcattcagct tttcggtgac ctcacgtacc gcatcaatgc ccagcatatt
    40801 tttccctttt tcgggagcca gggtgtagta atcgggatgc gtgccagcct gcatcaactg
    40861 acatccacga cagtgaccgc aacttttgtg gccctgcggt tgttggcaga gtaaataacg
    40921 gctcagggcg tagattaaag catcatcgcc catgcccggt aacgcctgaa tgagtagcgc
    40981 atggtgacct cttccggcct gatagctggc taccagtttt tcgaaatcag gtcgtaacca
    41041 tggataccat ctcatgcgtc caactcctta acccagtggg tcacggtagt gcggattgca
    41101 tccatcacgg cctccagcgg ctgggtggca tcaatggtat gaatactttt atcttgtgca
    41161 gccagttcca gatagcgggc gcgggtgcga ttaaagaaat cgaaagactc ttgctcaata
    41221 cgatccagct cgccgcgcgc acgcgcgcgt tttaagccaa cttccggggt aacatcgaga
    41281 tagagcgtta agtcggggcg aaaatccccg agaacagcat cacgcagtgt tgccagcata
    41341 tgttggtcaa taccacgtcc tccgccctga tacgcctgag tggagagatc gtggcgatcg
    41401 ccaatcaccc aggtgccgtt agccagagct ggtttgatga ccgtttctac cagttgaacg
    41461 cgcgcggcat aaaacatcag aacttcggct ttatcggtaa tgacttcatc gcctaccgat
    41521 ttgatatcca gcaccaggct tcttaacttt tcggcaagtt gcgtaccgcc aggttcccga
    41581 gtgaaaacca tgtcgcggat acccagttgc tcaagcgtct caaccaccac attacgcgcg
    41641 gtagttttgc ctgcgccttc cagcccctca atgacgatat acttactgcg catttttttc
    41701 cttaagcact ttcagataat cctgcacaga cttgttatga ctggcaagat tggtattaaa
    41761 cgtgtgacca cctttaccat cggccacaaa atagagatac ggcgtttttg ccggatgcgc
    41821 agcagccttc agcgaatccg cccccggcgt agctatcgca cccggcggca aaccggtaat
    41881 ggtataggtg ttatacgctg tcggcgtttc caggtctgca cgagaaagtt tgccattata
    41941 acgctctccc atcccgtaaa tcacggttgg gtcggtctgc aggcgcatac caatgcgtaa
    42001 acggttgata aataccgagg caacctgatc gcgttcactg gcaacggcgg tttctttttc
    42061 gataattgat gccatcgtca ccagctgatt tttatcttta taaggcagac cgtccgcacg
    42121 cccttcccag gcgctatcga ccgctttcac cattttcttg tgcgctcgct tgagtaacgc
    42181 gacatcggtg gtattggcgg tatacatcca ggtgtctggc cagaaccaac cttcaatcca
    42241 ctccgggttt tccagttcaa gtgcctgcgc tacggtggcg tacttatcgt cactcagcgt
    42301 gtgcttgata tacggagcct cacgcaattg cttgaggtag tcgctcagac gcatcccttc
    42361 taccagtcgc agcgggaact gtgcttcttt accgctttcc aacaatttca gcatctcgcg
    42421 cacggtcatc tgcggtgtaa agcggtaagt cccggcttta aagtgagaaa gatccggttc
    42481 gatacgcagc agccattgaa aaacccgtgg gcgattgatg atcttatcag cataaagctg
    42541 ttcaccgagc gccagacgtc cggtccctgg cttcagggta aatatcgtct cttctttgat
    42601 aagcaatttg ctgtcggcaa gatggcgaac cttccagacg cccacaccag cggcgatacc
    42661 cagtaccacc agcaataaca agattatcaa taacactttt ttcatgacta attcgggcgc
    42721 tcacaaagtg gggctaaata ttcatataac gttgctgacg aaaaggagac atcgccacag
    42781 gcacgtacgg gcatcactgg cattaacgca ttacaaataa ccatctcatc tgcctgcaac
    42841 gcctcttcca gagaagcttg cacttcgaca agctgataag aggattgtgc cagcaaacgg
    42901 atacagaatt gtcgcataat gccgttaaca cctgcctgat ccagtcgcgg cgtataaact
    42961 acgttgccct tccgccagaa caaattagcc gcacagcatt ccgtaaccca cccttcgctg
    43021 tcaaggacca gcgcctcatc agcgtttgtc tgctcaagat gagagcgaat caatacttgc
    43081 tcaagacgat tgagatgttt aatacctgca agatgaggat tgcgccccag ccgcaccggg
    43141 cttagcgcca acgtcatccc ctcgttacgc aaacggtcgt aatgtgcagg ataagccgta
    43201 acggagagaa tccgcgttgc cggtccactg ttcaatgtgc tgtaccctcg cccgccacta
    43261 ccgcgactga tcacgacttt cagtacacca ttttgctgtt ctgctgccag cgttttcatc
    43321 tcttgttcaa gctgtggcca gaagtcacag gaaatcatca accgctgaca agcatcctgt
    43381 agtcgctgga tatgcgccga taacaaactg actttaccgt cgataactct ggcggtggta
    43441 aaacaaccat caccaaactg cgttgcccga tcgcttactg ccagcgattc ctgcttataa
    43501 ccgttaatta agaacatacc ggctccttat cagagaattg ttagtgtggc agcatgttca
    43561 ctacggaaca agtcggaata aaagctaaga aaaaaggccc gcaagcggac cttttatatg
    43621 ggtggaaaat gacaacttag atctttttaa agatcaaaga accattagtg ccaccgaagc
    43681 cgaaggagtt acacagcgtg tattccattc cgctgacctg acgtgcttca tgcggtacga
    43741 aatccagatc gcaaccttca tccgggttat ccaggttgat ggttggcgga acagcctgat
    43801 cgcgcagtgc caggatggag tagatagatt ctactgcacc cgccgcacct aacaggtgac
    43861 cggtcataga tttcgtggag cttaccaaca cacggcttgc agcttcaccg aagatggttt
    43921 tcaccgcctg cgcttcagct ttatcgccag ccggcgtaga agtaccgtgc gcgttaacgt
    43981 agccaatctg actcgcttca atgcctgcat cacgcagagc atttgccatt gccagagctg
    44041 cgcctgcgcc attttctggc ggtgacgtca tatgataagc atcgctgctc ataccaaagc
    44101 cgacgagttc agcgtaaatt ttcgcaccgc gttttttcgc gtgttcgtac tcttcaagta
    44161 ccagcatacc ggcaccatcg cccagtacga agccatcacg ctctttatcc cacgggcggc
    44221 tcgccgcttg cgggttatca ttgcgggtag ataatgcacg tgccgcgcca aaaccaccaa
    44281 cacccagcgg cgtactggct ttctctgcgc cacctgcaac catcacgtca gcatcgccat
    44341 acgcgataat acgcgcagca tgaccaatgt tgtgcacgcc ggaagtacag gcggtcgcga
    44401 tagagatgct tgggccacgc aggccataca tgatagtcag atgacctgcc accatgttca
    44461 caatcgttga cggaacgaag aatgggctga tcttacgtgg accaccgttc atcagagatg
    44521 tgtggttttc ttcgatcagt ccgaggccgc caatcccgga gccaattgcg gcaccaatgc
    44581 gggttgcgtt ctcttccgtt atttcaaggc cagaatcctg catggcctga acgccagcga
    44641 caattccata ttgaatgaag gcatccatct tgcgctgttc tttgcgcgag ataatgtcct
    44701 cacagttaaa atcctttact aagccagcaa atttcgttgc ataggcgcta gtatcgaaat
    44761 ggtcgattag gctgatgcca ctctgaccgg caagcagagc tttccaggta gactctacgg
    44821 tattgccgac aggagacaac atgcccagtc cggtcacaac tacacgacgc ttagacacgt
    44881 ttgtcctcca gggagggaaa aaatgattct agtgggacaa aaagataaaa ctcaggcggt
    44941 cgaacgaccg cctggagatg ttcacttacg cctggtggcc gttgatgtaa tcaatggcag
    45001 cctgaacggt ggtgattttc tcagcttctt cgtccggaat ctcagtatca aactcttctt
    45061 ccagagccat taccagctca acggtgtcaa gagaatccgc gcccaggtct tcaacgaaag
    45121 aagcattgtt ggtaacttct tcctgcttaa cgcccagctg ttcgccgata attttcttaa
    45181 cgcgttcttc gatagtgctc atactcttaa atttcctatc aaaactcgct ttcgcgatgg
    45241 ttttcgtagt gtataaaatg ttgaaaaatt tgcaactaaa tcccggcagg tcttaccacg
    45301 attttacgtt attttgaggc cttacccccc aataacgcaa atcattttgc actaattatt
    45361 actcataacc acgcagactg cgcgcaacat gagcattttg tgcaaatcgc ggtcagacca
    45421 tgtacatccc gccgttcaca tgcaaagttt cacccgtgat gtaagctgct tcgtcggatg
    45481 ccaggaatgc aaccgcgttg gcgatttcct gtgcgccgcc gaggcgaccc gcaggaacct
    45541 gcgccaggat acccgcacgc tggtcatcgc tcagcgcacg tgtcatgtcc gtttcaataa
    45601 agcccggagc aaccacgttt acagtaatac cgcgtgacgc aacttcgcgc gccagtgatt
    45661 tactgaagcc gatcaagccc gctttcgccg cagcgtagtt ggcctgaccg ccatttccca
    45721 tggtaccaac cacagaaccg atagtgataa tacgaccatg acgctttttc atcatagcgc
    45781 gcattaccgc ttttgacaga cggaaaacag atgaaaggtt ggtttcgata atatcgttcc
    45841 actcttcatc tttcattcgc attaacaggt tatcacgagt gataccggca ttattgacca
    45901 ggatatccac ttcaccaaat tctgcgcgaa ttttttccag aacagattcg atagatgccg
    45961 ggtcggtcac attcaacatc agacctttgc cgttagcacc taaataatca ctgatcgcct
    46021 gagcgccatt ttcactggtc gcagtgccaa taactttcgc gccacgggct gcgagcgttt
    46081 cagcaattgc gcggccaatt ccgcggcttg caccggttac cagtgcgatt tttccttcaa
    46141 aattcatgat tttcctcttt taaagctcga gcgccgctgc catcgctgaa ggttcgttca
    46201 gcgccgaggc ggtcagggtg tcgacaatgc gtttcgtcag gccagttagc actttgcccg
    46261 ggccgacttc atagagatgt tctacgcctt gcgctgccat gtactcaaca gacttcgtcc
    46321 actgaaccgg gttatacaac tgacgtacca gtgcgtcacg aatggcatca ccattggttt
    46381 cgcatttcac atcaacgtta ttcacaacag gaactgttgg tgcgttaaag gtgattttcg
    46441 ctaattctac tgccagtttg tcggctgctg gtttcatcag cgcacagtga gacggtacgc
    46501 tcactggtaa cggcagagcg cgttttgcgc ccgcagcttt acaggcagcg ccagcacgct
    46561 caaccgcttc tttatgaccg gcaataacca cctgtcccgg agagttaaag tttaccggag
    46621 aaacgacctg accttctgcg gcttcttcac acgctttcgc aatagacgca tcatccagac
    46681 cgatgattgc cgccatagcg cccgtgcctt ctggtacggc ttcttgcatg aacttgccgc
    46741 gcatctcaac cagacgcacc gcatcagcga aatcaatcac gccagcgcaa accagcgcgg
    46801 agtattcccc caggctgtga ccggccatca ttgccggtgc tttaccgccc tgctgctgcc
    46861 atacgcgata cagcgcaaca gatgcagtca acagcgctgg ctgggtttgc caggttttat
    46921 tcagttcttc agctggcccc tgctgggtca gcgcccacag gtcgtagccc agcgccgcag
    46981 aagcttcagc aaacgtttct tcgacaattg gatagctcgc cgccatatca gccagcattc
    47041 caacggtttg agaaccctgt ccagggaaca caaatgcaaa ttgcgtcatg ttttaatcct
    47101 tatcctagaa acgaaccagc gcggagcccc aggtgaatcc accgccaaag gcttcaagca
    47161 gaaccaactg ccccggctta atgcgcccgt cgcgtacagc ttcatccagc gcgcacggga
    47221 cagaggccgc agaggtatta ccgtggcgat ccagcgtcac cacgacattg tccatcgaca
    47281 taccgagttt tttcgccgtt gcactgataa tacgcaggtt agcctgatgc ggaaccagcc
    47341 agtccagttg agaacggtca agattattcg ccgccagcgt ctcatcaacg atgtgcgcca
    47401 gttccgttac cgcaaccttg aagacttcgt tgcccgccat tgtcagatga attgaattct
    47461 ctggattcac gcggtcggcg tttggcagcg tcagcaattc accataacta ccgtcggcat
    47521 gcagatgggt ggagatgatt cccggctctt cagaggcagc cagcaccgca gcgcccgcgc
    47581 catcgccaaa aataataata gtcccacgat ctgttggatc gcaggtgcgc gccagtacat
    47641 cggaaccgac gaccagagca tacttcaccg ccccagattt cacgtattga tcggctacgc
    47701 ttaatgcata ggtgaaacct gcgcaggctg ctgcaacgtc aaatgccggg caacctttaa
    47761 tgcccaacat gctttgaatc tgacaagctg cgctcgggaa agcgtgcgta gcagaagtcg
    47821 ttgccacaac gatcaggcca atctggtctt tctcaatgcc cgccatctca attgcgcgtg
    47881 tcgccgcttc aaagcccatg gttgaaacgg tttcgtttgg cgcggcaatg tggcgttcgc
    47941 ggataccggt acgagtgaca atccactcgt cagaggtgtc caccattttt tccaaatcgg
    48001 cgtttgtccg cacttgttcg ggcagatagc tgccagtacc aataatcttc gtatacatgt
    48061 acgctcagtc acttttcggt tatataccgt cacttgcaaa ctgcgagttc gctggcagcg
    48121 tcctgctacc gcagagttcc gcttttgcca ccgtccagca gctcaaaacc agctgggtat
    48181 acagattcca ggcgagcggc aattcgctga ggaacttgtc gctgcaccgc ctgcactgcc
    48241 tgttcaatcg cgaccgcaaa agctcgctga ttggctgcac catgactttt tatcaccgtg
    48301 ccgcgcaatc ctaacagaca ggcgccgtta tactggtcgg ggttgaggtg actgaatcgc
    48361 ctcgtcaggc tcttttgtag ccaacgcttt aataacagta gccaccacga ccgttttttc
    48421 ccttcaccct gagatttcag cagagaaagg aacatcctga caacaccttc catcgtcttt
    48481 aacgtgacat ttcctgtaaa gccgtcacaa accagcacat ctgtcttgcc agttaacaac
    48541 tcattggctt caagatagcc gatataattg atagaaggga ttgttttaag caccgctgag
    48601 gcatcccgaa tactgtcgag accctttact tcttcttcac caatattgag caacgccacg
    48661 cgaggattgg gaatttccac cacctcttca gccagaactg agcccataat ggcaaattgc
    48721 accaacattg tgctgtcaca atcgacgttg gcccctaaat cgaggaccac cgttttgccc
    48781 ttttgctgat gtggtaatac cgtcaccagc gccggacgct caatcccctc caggggcttg
    48841 agtaataatt ttgccagccc catcagtgcc ccggtattac cggcactgac acaggcttgc
    48901 gctcgacctt ctttcaccag ctccagggcc atacgcattg aactcccacg actggcgcgg
    48961 atagcttgcg aaggccgggc atcactggcg ataactgact gcgcaggaat aatctgcaga
    49021 cgcgaacgtt gttcaaagtc agctttagca agtaatggcg tgatggcgtc gggattgccg
    49081 actaaaagaa gagtgagttg cgaattagaa ttcagtgcct gcaatgctgc aggcactgtc
    49141 acggaagggc caaaatcccc tcccatgaca tctaacgcca gggttagacg tgtcaaggta
    49201 tcgtcgccgc ccggtttggt ctttccccag ttgcctgggg aaaatcctca ctaagcttca
    49261 tcacgcagac gcgtgattac ttagcgatga ccttgcggcc gcggtagtaa ccgtcggcag
    49321 tgatgtggtg acgcaggtgt ttttcaccag aagttttgtc tacagacagg ctggtgactg
    49381 cggtcagcgc gtcatgggaa cgacgcatgc cacgtttgga acgggttggt ttattctgtt
    49441 gtacggccat ggaccttact cctcaattac ggtttatccc cgatccaacg gggagcacca
    49501 attacttacg ctttaagctg gctaatacgg caaatgggtt tggcttttgc gcttcttcag
    49561 gcagttcacc aaagaccatg tccgcttcgg acacttcaca gtgttcagaa tcatgcaccg
    49621 gaactaccgg caaggcgagg atgatttcat cttcaaccat tgcaagcaga tcgatttcac
    49681 cgaattcgtt aacctcaatc ggttcatacg cttccggcag tgcttcagcc tgttcgtctg
    49741 aacgcacagg actaaaacaa tacgttgtgt agacctgatg agtaaacggc ttcccgcaac
    49801 gctgacactc gagcgttacc gtcaccttcg catcgccgtt taacactgcg agacgttggt
    49861 tatcgatagc gaacgacatg gagcattcca catcactgtc cacactgact acggattcgg
    49921 cgacgcgctc aacctgatca ggggtataga taccctggta atcaaggcgt ttttgagccg
    49981 tacgaaccgg atcgagagtc aggggtaatt ttaccttttg catagggcgc gcatattaac
    50041 tttgtaacgt catagagtca aagaaaaagg cagcctgtgg ttgccttttg ccaataattc
    50101 gcacacattg cgggttactg ctttattttc gttcagtgct gataacgaac atcaacctgc
    50161 aaaacgaagc gcatagttta aaatggctat catcaatacg ctatatctga tggaaaaaaa
    50221 tatgcctaaa cttattttag cctccacttc gccctggcgt cgcgctcttc tggaaaaact
    50281 gcaaatctct tttgaatgtg cagcaccaga ggtcgacgaa accccacgca gcgacgaatc
    50341 accgcgacag ttggtgcttc gactggcaca agaaaaagca caatctctgg cgtcacgtta
    50401 tccggatcat ttaattattg gttcagacca ggtatgtgtt cttgatggcg aaatcaccgg
    50461 aaaaccgtta acggaagaaa atgcccgtct gcaattacgc aaagccagcg gcaatatcgt
    50521 caccttctat actggactgg cgttgtttaa ttcggcgaat gggcatctgc aaacagaagt
    50581 ggagcctttt gacgtccatt tccgtcacct gagcgaggcg gagattgata attacgtgcg
    50641 taaggagcat cccctgcact gcgcgggtag ctttaagagt gaaggatttg gcattacgct
    50701 gtttgagcgt ttagaggggc gcgaccctaa cacactggtt ggtttaccgc taattgcgct
    50761 gtgtcagatg ttacgtcggg aagggaaaaa tccgctgatg ggatagtttt ttgcgccgga
    50821 tgcggttacg tcgcatccgg caatcggtgc aggcggcctg atgcggcgta aacgccttat
    50881 caggccaacc gcccattatc aggcttatat gcttgtatta gcgcgcgtta cgcagctttt
    50941 gcaaacaacg cttcaaacct tcatccatcg gcgcttcgat gcgcatcacc tcaccggtcc
    51001 ccggatgggt aaacttcaac gctgcggcgt gcaggaacag acgatttaat cccgtgcctg
    51061 cttcagtgag ctgtctgtca aattcacggt caccgtagcg atcgtcaaag gcaatcggat
    51121 gacccgcata ctgtgtatgc acacggatct gatgagtacg tccggttacc gggctacaac
    51181 gcaccagggt ggcaaatgca tagcgttctt ccactttaaa gcgtgtttcc gacggtttgc
    51241 cttcctgact gacacgcacg atacgttcgc cgctttgcag aatatttttc aataacggcg
    51301 cttgaacgct cttcacatgc gactgccact gaccgcgcac cagcgccagg taatcttttt
    51361 gcattccttt ttcacgtaat tgctcatgca gagaacgcaa cgccgagcgt tttttcgcta
    51421 ccagcaacac acctgaagtg tcccggtcga gacgatgaac cagttcaagg aaccgcgctt
    51481 ccgggcgcaa cgcccgcaaa ccttcgataa cgccgaagct taaaccactg ccgccatgta
    51541 ccgccgtacc ggaaggttta ttcagcacca ggatatgatc atcttcatat aagatgacgt
    51601 ccgccagcgc cgccaccttt tgcagatgtg gcgaaaccgc ctcttcttcc cgctcagcaa
    51661 cgcgaaccgg tgggatacgc acctcatcac ccgcttcgag tttatattca ggcttaatac
    51721 gttttttgtt cacccgcact tcacctttac gcaaaatacg gtaaatcata ctttttggta
    51781 cgcctttcaa ttgggtacgc aaaaagttat cgatacgttg ccccgcttcg tcagcggtga
    51841 tagcaacaat ttttacggat ggagtctctg ttttcatggt gggcgattct aattagccaa
    51901 caggattcgc gccactcatt tttctacgct tatatttagt tttgcacctt attacttcac
    51961 tacgtgatca ctttattgat ggttattaaa ccaatcacca gcaagaagtg aaaaaactgt
    52021 gagtaagcgg gtgataaatg gtaaaagtca tcttgctata acaaggcttg cagtggaata
    52081 atgaggccgt ttccgtgtcc atccttgtta aaacaagaaa ttttacggaa taacccattt
    52141 tgcccgaccg atcatccacg cagcaatggc gtaagacgta ttgatctttc aggcagttag
    52201 cgggctgcgg gttgcagtcc ttaccggtag atggaaatat ttctggagag taatacccag
    52261 tctgtttctt tgataattgc gctgtttttc cgcatgaaaa acgggcaacc gacactctgc
    52321 gcctctttga gctgacgata accgtgaggt tggcgacgcg actagacacg aggccatcgg
    52381 ttcacacccg gaaaggcgtt actttgcccg cagcttagtc gtcaatgtaa gaataatgag
    52441 taagttacga tgaaaagaat gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc
    52501 cttgtagatg ggcagcgtct gtatgacctg gatatcgaaa gtccagggca cgagcagaaa
    52561 aaggcaaaca tctacaaagg taaaatcacc cgcattgaac cgagtctgga agctgctttt
    52621 gttgattacg gcgctgaacg tcacggtttc ctcccactaa aagaaattgc ccgcgaatat
    52681 ttccctgcta actacagtgc tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt
    52741 caggaagtca ttgttcagat cgataaagaa gagcgcggca acaaaggcgc ggcattaacc
    52801 acctttatca gtctggcggg tagctatctg gttctgatgc cgaacaaccc gcgcgcgggt
    52861 ggcatttctc gccgtatcga aggcgacgac cgtaccgaat taaaagaagc actggcgagc
    52921 cttgaactgc cggaaggcat ggggcttatc gtgcgcaccg ctggcgtcgg caaatctgct
    52981 gaggcgctgc aatgggattt aagcttccgt ctgaaacact gggaagccat caaaaaagcc
    53041 gctgaaagcc gcccggctcc gttcctgatt catcaggaga gcaacgtaat cgttcgcgca
    53101 ttccgcgatt acttacgtca ggacatcggc gaaatcctta tcgataaccc gaaagtgctc
    53161 gaactggcac gtcagcatat cgctgcatta ggtcgcccgg atttcagcag caaaatcaaa
    53221 ctgtacaccg gcgagatccc gctgttcagc cactaccaga tcgagtcgca gatcgagtcc
    53281 gccttccagc gtgaagtccg tctgccgtcc ggtggttcca ttgttatcga cagcaccgaa
    53341 gcgttaaccg ccatcgacat caactccgca cgcgcgaccc gcggcggcga tatcgaagaa
    53401 actgcgttta acaccaacct cgaagctgcc gatgagattg ctcgtcagct acgcctgcgt
    53461 gacctcggcg gcctgattgt tatcgacttc atcgacatga cgcctgtacg ccaccagcgt
    53521 gcggtagaaa accgtctgcg tgaagcggtg cgtcaggacc gtgcgcgtat tcaaatcagc
    53581 catatttctc gctttggcct gctggaaatg tctcgtcagc gcctgagccc atcactgggt
    53641 gaatccagtc atcacgtttg tccgcgttgt tctggtactg gcaccgtgcg tgacaacgaa
    53701 tcgctgtcgc tctctattct gcgtctgatc gaagaagaag cgctgaaaga gaacacccag
    53761 gaagttcacg ccattgttcc tgtgccaatc gcttcttacc tgctgaatga aaaacgttct
    53821 gcggtaaatg ccattgaaac tcgtcaggac ggtgtgcgct gtgtaattgt gccaaacgat
    53881 cagatggaaa ccccgcacta ccacgtgctg cgcgtgcgta aaggggaaga aaccccaacc
    53941 ttaagctaca tgctgccgaa gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag
    54001 ttcgctgaac gtaagcgtcc ggaacaacct gcgctggcaa cctttgccat gccggatgtg
    54061 ccgccagcgc caacgccagc tgaacctgcc gcgcctgttg tagccccagc accgaaagct
    54121 gcaccggcaa caccagcaac tcctgcacaa cctgggctgt tgagccgctt cttcggcgca
    54181 ctgaaagcgc tgttcagcgg tagtgaagaa accaaaccga ccgagcaacc agcaccgaaa
    54241 gcagaagcga aaccggaacg tcaacaggat cgtcgcaagc ctcgtcagaa caaccgccgt
    54301 gaccgtaatg agcgccgcga cacccgtagt gaacgtactg aaggcagcga taatcgcgaa
    54361 gaaaaccgtc gtaatcgtcg ccaggcacag cagcagactg ccgagacgcg tgagagccgt
    54421 cagcaagctg aggtaacgga aaaagcacgt accaccgacg agcagcaagc gccgcgtcgt
    54481 gaacgtagcc gccgccgtaa cgatgataaa cgtcaggcgc aacaagaagc gaaggcgctg
    54541 aatgttgaag agcaatctgt tcaggaaacc gaacaggaag aacgtgtacg tccggttcag
    54601 ccgcgtcgta aacagcgtca gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa
    54661 gaagcggtag tcgcaccggt ggttgaagaa actgccgctg ccgaaccaat tgttcaggaa
    54721 gctccagctc cacgcacaga actggtgaaa gtcccgctgc cagtcgtagc gcaaactgca
    54781 ccagaacagc aagaagagaa caatgctgat aaccgtgaca acggtggcat gccgcgtcgt
    54841 tctcgccgct cgcctcgtca cctgcgcgtg agtggtcagc gtcgtcgtcg ctatcgtgac
    54901 gagcgttatc caacccagtc gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactg
    54961 gcctctggca aagtctggat ccgctatcca attgtacgtc cgcaagatgt acaagttgaa
    55021 gagcagcgcg aacaggaaga agtacaagtg cagccgatgg tgactgaggt ccctgtcgcc
    55081 gccgctgtcg aacctgttgt tagcgcgcca gttgttgaag aaatggctga agtcgtagaa
    55141 gcccccgtac cggttgccga accgcaaccg gaagtggttg aaacgacgca tcctgaagtg
    55201 attgccgccg cggtaactga acagccgcag gtgattaccg agtctgatgt tgccgtagcc
    55261 caggaagtcg cagaacacgc agaaccggtg gttgaaccgc aggaagagac ggcagatatt
    55321 gaagaagttg ccgaaactgc tgaggttgtg gttgctgaac ctgaagttgt tgctcaacct
    55381 gccgcgccag tcgtcgctga agtcgcagca gaagttgaaa cggtagccgc ggttgaacca
    55441 gagatcaccg ttgagcataa ccacgctacc gcgccaatga cgcgcgctcc ggcaccggaa
    55501 tatgttccgg aggcaccgcg tcacagtgac tggcagcgcc ctacttttgc cttcgaaggt
    55561 aaaggtgccg caggtggtca tacggcaacg catcatgcct ctgccgctcc tgcgcgtccg
    55621 caacctgttg agtaataatt agctcaaagt aatcaagccc tggtaactgc cagggctttt
    55681 ttatttcatc tttgaatcaa catattaaaa ttcatcaaat ggcatccttg ctaaccaaca
    55741 atgcaaaata ggcaaaaaaa atgcccgccc cggcgatccc agagcaggca gacaaaacat
    55801 acccagtttc atgatatgtt ttaaagcgag cttatttgtt gagctggaag agcgacaatc
    55861 cctgcatatc ggtaaatgct ttatacgatg cctgcaatgc cgtttgctgc atgatgtaag
    55921 atgaaatagt tgcattccag tccacatcaa ccagatcgct catctgctgc gtttgcccta
    55981 aagcgcgatc gctacctaag gaatccagcg actccagttc gttcagctgc gtgcctaatt
    56041 ccgcgcgaac agtcagcaca ttgttcagcg agtttttcag tccgcggttg gttttatcta
    56101 acgccgcagc ggcggtttct ttatccgctt cgctatccgc gaccggcgtt ttcagggctg
    56161 cgatggcact atccagcatg gcaaaaagat tggtttcaga agcgctaccg tctggttccg
    56221 ctaccgcgtt gctggtaata ctgtcgaaaa ttttgtcacc cgtgtgccct atcaccatcg
    56281 aacgcgaagc atcgacctgt tgtttaatac tttctgctcc accgacgtat ttccctttct
    56341 cttcgctaaa cggcgcagtc tctgttttat aaccggcaaa aatgtagcgc ccgttaccgt
    56401 cagtggtgtt cgccagattc agcaactggt cacgaagccc ctgaatatcc gtagccagcg
    56461 aggcccggtc atcgtcactc aaggtgccat tgctggcgta gacaattttt tcctgagcat
    56521 tctggatagc agtggtgacc tggctaagta cactctcttc cagtgacact ttttgagtgg
    56581 cgaaagtacg tgccagcgtg tactggctgt tttgcgcctg tgcctgggag agaacaacag
    56641 cttgtgatgc agcaatggga tcgtctgaag ggttaacgac tcgcttaccc gtcgacatct
    56701 gctcgccgta cttcatccat tctgcctgag aattggtgat gccgcgcatg ttttgctggt
    56761 acatcatctg ggtactgaaa cgcattttat ctccccttag cgaatgttaa tcagcgcatc
    56821 aaaaatcgcg tttgccgtct gcagaacctg cgcattcgcc aggtaatact gctgaaaacg
    56881 ttgcagattt ccgtactcct catcgagatt gacaccggaa atcgactgct gctgattgga
    56941 aagctgcgtc accacattac cttgcgtggt gctactggtt ttcaacgtcg cggttttatt
    57001 accgatatca ctcactaacg aggcataagc gtcgttaaag gatttcgcac cacccaccgt
    57061 tttactgttg ctttgcagat ccagcagggc ctgaccgttg cggttgtcgc tatcacccgc
    57121 atcttcttcg ctcgccatcg cgattttcgc ttcgtcggtg attaatacat ccatgttgac
    57181 gatggcgtca cttactggtt tcagcgtgaa gctgtcgtta acggcaggcg ttcctgtaaa
    57241 cgtcaactcc agaccatcaa atgccacttt accgttggca tctggtgtcg ccgtaaaagt
    57301 ggtattgctg gcaaggcggg tgacctgcca ctgattatta tcgaacgaga ttttgtaatc
    57361 tgtcgccagt accacggagg catcagttac cgtggcaccg atcgcaacgt cacctttgtt
    57421 tttcgtgttt tgcagaaccg cgggcttacc gatagtaaag aaatcttcac cagcatcgcc
    57481 gttagcatca aatccggctt tgtgttggct gttgaaagcc tcggcaaatg ccagcgccag
    57541 ttgtccaagc gtattacgcg tctggtccag atcctgagaa cggaatgtca gaatgccgcc
    57601 cagcgacccg gtattcagta atttctccgg gatctcaata ttgcctgccg tcccatcaac
    57661 gtaagcgaca gtcgtacgag aagggtcggc gctggaagga actgccgcca gttgccgcgc
    57721 cgtacttccc tgaaccagtg agtaaccatt ggccatcgtg atgttataag tgccgccatc
    57781 ctgaacgctg acttctacac caacaatctg gtttaattcg ctcaccagtt gatcgcgttg
    57841 atccagcaga ttgttaggtg acgcccctgc ccccacgcct gtcaggcggg agatttgatc
    57901 gttcaggctg gcgatttgtt tagcgtaatt gttgatctga tcaacgctgg cacctatcgc
    57961 gatattgacc tgtttgtcct ggtcgcgcag atattgatcg gtggttttaa actgattcac
    58021 caacccttct gatttcccaa tcagcgcctg gcgcgctgcc gggtcttccg cgttactcac
    58081 cagcgtttgc aggctggtga agaaatcctg catctgtgtt gccagcgaag aggtactggt
    58141 ggagagcata ttgtcgattt tcgacatctg ctcatagcgg gcagtcaggc cgctactttg
    58201 cgtctgcgcc gcacgtaact ggttggtaat aaaagcatca tactcacgct gcacaccaga
    58261 aacgtagaca ccattgccaa cccagccgcc agcgcccaac gtgctattgg cctgcgccat
    58321 aatagtggtt tggcgggtat atccggcaac gttatagctg gagatattat tacttgccgt
    58381 atttaacgcc gcctgggccg cgttcagtcc gctcatggcg ttattaatca agctggacat
    58441 ggaggttcct tataagcctt caattacaga gtattatcgg cagcgacccg ccggacttga
    58501 gttattcaga acagattatc aatgttcatg ctgtaggttt tgctcacctt gtcgcttatc
    58561 gatttcatct gctgaatcat gttggtgagt ttgcgggcat agtgaggatc ggtggcatag
    58621 cccgcgtcct gtagggcctg cgccccctgt tccgcactcg cggcggtcgt cacggcggcg
    58681 tagcgcgggt tacgcgttaa cagcccaacg taatccgaca aggcttccag atacgagctg
    58741 tagacgcgaa acttcgcttt tactttcttc gcttcgccgt tttcatattc agtcgtggtg
    58801 atttcagtaa ctggcccttt ccagttgcca gaggctttga caccaaacag gttatagctc
    58861 ggctcgccgt tttcgcggcg gatttgccgt tgcccccaac cagattccag tgccgcctga
    58921 gcgaggatca aatgatgtgg cacaccgctt tgctggcttg ccagttgggc gggcagcgag
    58981 agttgcgcga ggaatgcttt actgtcaccc ggcagcgaat catcgtagtt acgtggcacg
    59041 gccttttgca ccagctgcga aagcgcctga ttttgataac gcaccacggt ttcgagcggg
    59101 aatttcatcg gtgctgctgg cgtggactcc tctggcaatg gttgttctgg cgtcatctgt
    59161 ttaaccatca tctctgcaag ccccagacct ttgcccgccg tcatctgttg ggcaatttgc
    59221 tggtcataca tactggtata cagacgagtg tgctcgctgc tgaacaggcc atcttttggt
    59281 aaagcgtcgc gcatgctttt caacatcatc tgcacgaaca tcccttccac ctggcgggcc
    59341 accggacgga tatttgccgc cggatcttcg cccgctttcg cctttagttc gttgagtgat
    59401 tgcgcatccc aggccgcact tgccagtagt ttgctgtcgc tgatcatcag atgatttcca
    59461 gttttgcccg cagacatccc gcactttgca ttgattgcaa aatagacatc agatccatcg
    59521 gcgtagcgcc cagcgcattg agcgcgcgca ccacgttatt gaggctggcg ctggaacgta
    59581 cgctttgcag cgaaccgccg ctctggcgta aatcgatctg cgtttgtgga gtaaccacag
    59641 tctgtccacc accaaacggt gtatctggct ggctgacatt ggcctgacga ttgactgtta
    59701 ctgagagatt tccctgcgct accgcgcagc tgtcgagggt cacttcgcga ttcatcacca
    59761 ccgaaccggt gcgcgagtta atcactactt tagcgtcctg cggggtgaca ttaacctgca
    59821 tattctggat atctgcaagg aaacggacct gggaactgtt gccacttggt acgcgcacct
    59881 gaatagtccg cgcatctaac gcggtggcgc tgccatatcc acgcacgcga ttgatggtgt
    59941 cagcgatttg ctgcgccatg ctgaaatctt cgtcgttaag ttgcaaatta agggtattcc
    60001 cgacgccaaa ctggctgggc aattcacgtt caataaccgc accattggtg atccgtccac
    60061 cgttcagttg gttcacctga acactgctac cgccagcgga ggctcctgcg ccgccaacca
    60121 gaatattgcc ctgcgccagc gcatacacct gactgtcaac gcccttaagc ggcgtcatca
    60181 acaacgtacc tccacgcaag cttttggcat ttcccatgga agaaaccacc acatcgatgg
    60241 tttgcccctg acgtccaaac ggaggaagtg acgctgtcac cattaccgca gcgacgtttt
    60301 ttagctgcat attggtgccc gtcggaacgg taattcccag ctgtgagagc atgttattaa
    60361 gcgtttgtgt ggtaaacggc gtctgggttg tctggtcacc ggtgccatcc agccccacca
    60421 ccagaccata gccaatcagt gagttttgcc ttaccccctg aacactggtg agatcgcgaa
    60481 tacgctcagc ctgagccgcc gtcgtaacca gtagaagaat taatgcagag agaaatttaa
    60541 tcaccacagc ctcacttaca ttggcgacag gttaaggaag aaacgctgca accagcccat
    60601 attttgcgct tcgttaatgt agccattgcc tacgtattca atgcgcgcat ccgccacctg
    60661 agtagacggt acggtattgc tgccgctgat agtgcgtgga ttaaccacgc cagagaagcg
    60721 aataaattcg gtaccctgat taatggcaat ctgtttttca cccaccacat gcaggttgcc
    60781 gttgaccagt atctggtcaa ccgtcaccgt caacgtgccg ctaaaggtat tgctggcatt
    60841 ggccccgccc tttccgttga acgtgttacc accggaggct tcgacatcgg cacgagcgtt
    60901 accaaacagc ccctgcaaat agcgcggcac agtatcaaag ccaaaattag ttttaccgtc
    60961 acggctggca ttcgcagagg agcttttgct ggcgctgacg ttctcctgca acacgatggt
    61021 cagcgtatcg ccaatattgc gtggtcgacg atcttcaaac agcggttgat agccatagtt
    61081 aatcggctga gcagactgga aaatagaacc gttggcgacg ggcgtcggcc cgggaaccgg
    61141 ttgtgcactg gtcgccccct gcaccagcgg cgtggagggt atccaggcgc agccggttag
    61201 tgaaagcacc aacaagctgg aaatggcata agtatgcgca gcgttttttt gcatggctat
    61261 catcttcaaa aatcagtaaa ccggtgaacc tgccaccggt taagccttag agttgcgtca
    61321 gtttttgcag catctgatcg gtggtggaca ccgctttact gttgatttcg taagcgcgtt
    61381 gcacctgaat catattgacc agttcttccg ccacgttgac gttagacgtt tcaacatacc
    61441 cttgatacag cagtcccgcg ccgttcaggc ccggcgtgct ttcgttcggt gcaccagagg
    61501 attgcgtttc ggtgtagagg ttttcgccaa tgctctccag cccggtgtca ttcataaagg
    61561 tggtgagatt gagctgccca acctgaaccg gagctgcctg gccttgttgg gttacgctga
    61621 ccacgccatc acgaccgatg gtgatactta acgcattcgc cggaatggtg atcgctggct
    61681 gcacctgaaa accaccagcc gtcaccagct gcccgttctg atccacctgg aaagagccgt
    61741 cacgggtata ggctgatgaa ccatctggca acatcacctg gaaaaagccc tgccctttaa
    61801 tcgcgacatc tttgctgttg ttggtctgcg acaggtttcc ctggctgtgt aagcgttcag
    61861 tggcgaccgg gcgtaccccc gtgccgattt gtaatccgga gggtaaggtg gtttgttcgg
    61921 aagactgtgc ccccggctgg cgaatggttt gataaagcag atcttcaaac accgcgcgct
    61981 gacgcttaaa accgttagta ctgacgtttg ccaggttgtt ggcaatgacg tccatattgg
    62041 tttgctgggc gtcaaggccc gttttggcga tccataatga actgatcata aaatgtcctg
    62101 tagtggatta gtgacaagca gagcagaagc acgcgacagt gtgaatttcc cctcacccta
    62161 accctctccc caaaggggcg agggaatagt ccgcgcgctt ttattgcaat gtcatcaatc
    62221 gttcaattaa ctcatcgaca gcagttggtt ggcacggcct gcgttatcat cgacgctgct
    62281 gatcaccttc atctgcattt caaaacgccg cgcgctggca atcatgtcgc tcattgccgc
    62341 aacggcattg acgttactgc cttccagaac ccccgacatc acacgcaagg ttggatctgc
    62401 ctgcagtatc ggcccacgcg tggcctggct ttctgcgctt aaacgaaaaa tgccgtcgtc
    62461 accgcgttgc acttcgctgc ccgtggcttt caccagtttc agacgcccca ctggcgcaac
    62521 cgtatttgcc ggatcgcccg gattgagcgc cgagattgtg ccatcggcag caatagtgat
    62581 ttccgcccct tccggcacag caattggccc agcctcgcct atcaccggat gcccctgaat
    62641 tgtcagttgc ccggtgggat caacctgaat gctgccatta cgcgtatacc cttcgctgcc
    62701 gtcagcggac tgcacggcca gccagccatc ctgctgcaac gcaacgtcca gcgggcgcga
    62761 ggtgtaatcc atcttgccgg gcgtcatgtc tgcacccggt gttgacgccg ttaccagcgt
    62821 gcgcgtgggc agtgaaagcc cttccaccgg cactgcacgc aaggcgttta actgcgcgcg
    62881 aaaaccgggc gtagaggcat tggccagatt actggcggtt accgcctgtt gattcagcgt
    62941 ctggctggct gctcccatcg cggtataaat tgcgtgatcc attgagctat cccgtcagcg
    63001 attagcgtaa gttaaccagc gtgttgagga tctggtcctg ggttttgatg gtctgggcgt
    63061 tagactgata gttacgctgg gcaacgatca tattgaccag ttctttactg agatcgacgt
    63121 tggacgcttc caacgcaccg ttggtcaggg tgccaaagtt gcccgtcccg gctgtcccca
    63181 acagcgccac gccagaagat tgcgtcgcag accagacgtt gtcgccttcg gatgccagac
    63241 cttcgttgtt ggcaaagttc gccagtacaa tctgccccag cagttgtgtt tgttcgttgg
    63301 aatagttgcc gacaaccgta ccgtcatcat tgatttgata actcaccaga tcgcccggtt
    63361 tgtagccatt ctgggtggtt gccacaatat tgttagcgcc ggtattttgc tgcatggagt
    63421 tgaggaagct caggctaaac gtggcgggat ctgcgccgtt aattgcgcca gtggtaatat
    63481 tctctgttgg atttgaggtc agaacgccat tggcattaaa caccagcttc attgcaggct
    63541 cggctgtacc tgttggatca ctgctatcct gggtgtagac ctgccagtta ttatccccgg
    63601 tcttcacaaa gtagacgctc atgtcatgag cattaccctg actgtcgaaa acagtcaccg
    63661 aacctttttt gttatagcta tccgcattgc tggcgctgaa tggcgtaaca gtaggaagcg
    63721 gatcactgga attcaggttg atctgcatcg atgccgtggt ggtagttttc gctgccatca
    63781 gggtattcgg gatcgaaata ttggtcggat tcgccccttg ctgaatagtc ggcggcgtac
    63841 cggttgccgg gtaacccgtc agctgtaaac cttgcatatt caccaggtta cggttttcat
    63901 ccagcttaaa ttgtccgtta cggctgtaga acaccgaacc gttgctgtct accagacgga
    63961 aaaaaccgtt ctggctgata gcaacgtcca gacctcgccc ggtgttggtg gtcgtgccat
    64021 cggtaaagtc ctgagtgata ccggcaactt ttacccccag tcccactttc gaaccggcaa
    64081 acatatcggc aaaagaggcc gtgcctgatt taaagccgta ggtggcggag ttggcgatat
    64141 tgttgccaat aacatcgagg ttggtggcgg cagcgtttaa tccgctaacc gcttgagaaa
    64201 aggccatgac tgactcctga aatgtgaagg cttaaattat ctgccgtact tcgtcgaggg
    64261 tggtggtgcc gtaagtgccg agatccagcg tattaccgcg gttgccgcgg atcacaccct
    64321 gcaccagagc aaactgcagc ggctgggcaa ccagttgtgt accaccgtta ctcgcgctaa
    64381 tcgctacatt gtaagaaccg ttcggcgcag tgctgccgtc agtcaacgta ccgtcccagg
    64441 tgaaactgtg aactccggcg gtcagttcac caatatcaat ggtgcgcaca accgcaccat
    64501 ttttatcggt gatggtggcc gtaactttgt ctgccgcctg ttgcagctca acaccaaacg
    64561 gcgtggtcgt ggtcacagct ccttcttcac tgccggttcc cgcaagaaca gtggtgccgg
    64621 ggatcatcac gccgtgaccg atcaggttac tggcctgtaa cgactggctg ttatcaatct
    64681 gtccggaaat agatccgagt gtggtattga gtttttcaat cccgctgacc gtgctgattt
    64741 gtgccaattg cgacgtcagc tcgttgtttt ccattggatt ggtcgggtcc tggtttttca
    64801 gctgcgccac cagcaaagtc agaaaactgc tttgtaaatc tgcggcgttg ctgcccgtga
    64861 gcgaactact gctggtggta ctgacgccgg tatttgtcgg atcggtggtg gttaccgcaa
    64921 tggacatagc tttctccttt attgaccgag cgtaagggtt ttcagcatca tacttttcac
    64981 cgtgttgagc acttcaacat tggcctgata gctgcgtgac gccgacatgg tgttaaccat
    65041 ctccccgacg acatcaacgt tcggcatttt tacgtagccc tttgcatccg ccagcggatt
    65101 acccggttca taaaccagtt tgtccggggc ctgactttct ataacatcgg caacctttac
    65161 gccgcctgtc gcagcacctg gtgcagcgtt aacctggaat accacctgtt ttgcccgata
    65221 tggctgtcca tcgggaccgg tcacgctatc agcatttgcc agattactgg ccgccacgtt
    65281 caggcgctgg gactgggcag ttaacgccga cccggcgata tcaaaaatat tcagcagtgc
    65341 catccgttaa tttccgctct gtaaaacgtt catcatgcct ttgatttgcc cgctcaacgc
    65401 gctaaggctc atctggtatt gcaggctgtt atcggcaaac tgggtgcgtt cgcgatccat
    65461 atcgacggta ttaccgtcaa gcgaaggctg gtccggaata cggtattgca gttctgcggt
    65521 aggaggcgtc agcgcctgcg ccgggatgtg ttgcgttgag gtcatcgtca gtgcaaccac
    65581 actggttgca tcccgtccac gttgcatgac ttttttaagt tcactggcaa aatcgatatc
    65641 gcgcgcctga taaccagggg tatcggcatt ggcgatgttt gctgccagca cttcctgacg
    65701 ctgggcgcgc agattgagcg cctcttgttg aaaacgtaag gcggcgtcga gcttatcgag
    65761 catatctcct ccgcaggtat caaaattctg ccatcagctt aaatgccttt acgcgcgcgt
    65821 tatcggcgga ataaacgcaa aatgggtcgc tatttatgcc gttgatggtc attgcggaca
    65881 ggtacaattc acgttgtaga aatggctggg ggcgaaaatg ctgacaataa aacgtagcgt
    65941 ggcgatcatc gcgatactgt tcagtccgtt aagtacggcg agcaatctca catcgcaatt
    66001 gcacaacttt tttagcgccc aactcgcggg ggtaagtgat gaggttcgtg tttctattcg
    66061 tacagcgccc aatctactac cgccatgcga gcagccattg ctttcgatga gcaataattc
    66121 ccgcctgtgg ggcaatgtga atgtgttggc acgctgcggt aacgacaaac gatatttaca
    66181 ggttaatgtg caggccacag gaaattatgt ggttgccgcg atgcccattg tgcggggagg
    66241 aaagctggaa gctggcaatg tcaaactaaa acgcggacgg ctggataccc tgccaccgcg
    66301 tacggtgctg gatatcaatc aacttgttga tgccattagc ctgcgcgatc tatcacccga
    66361 tcaacctatc cagttaaccc agtttcgcca ggcatggcgg gtaaaagcgg gacaacgcgt
    66421 caatgtgatc gccagcggtg atgggtttag cgccaacgca gaaggtcagg cgctgaacaa
    66481 tgcagccgtc gcacagaatg cgcgggtgcg catggtatcg ggacaggtag tcagcggcgt
    66541 tgttgatgca gatgggaata ttcttataaa cctgtaagct gttaaagatt acccgtccct
    66601 tgccgataaa taagcaacac atgataaaag cgccctcaat gaggaataaa ccatgagtat
    66661 tgatcgcact tcgcctctga agcctgtaag caccgttcaa ccgcgcgaaa ccactgacgc
    66721 gccggtaacg aacagccggg cggcaaaaac aaccgcctcc accagcacca gtgtgacgtt
    66781 aagcgacgcg caagcaaaac tgatgcaacc cggcagcagt gatatcaatc ttgaacgtgt
    66841 cgaagcgtta aaactggcga ttcgtaacgg tgaactaaaa atggacaccg gcaaaattgc
    66901 cgatgcgctg atcaacgaag cgcagcaaga cttgcagagt aactgatcgt atgacacgtc
    66961 ttgcagagat cctcgaccag atgtccgctg tgcttaacga tctcaaaacg gtaatggatc
    67021 aagagcagca acatctctct atggggcaga tcaacggcag ccagttgcaa tggattacag
    67081 aacaaaaaag ctcactgctg gcgacgctgg attacctcga acagttacgc aagaaagaac
    67141 ccaatacagc aaatagcgtt gatattagtc aacgctggca ggaaattact gtgaaaacgc
    67201 agcaactacg ccaaatgaat caacataacg gctggttact ggaaggacag attgagcgca
    67261 atcaacaggc gctggaaatg ttgaaaccgc atcaggaacc gacgctatat ggggcgaacg
    67321 ctcagacctc aacaacccat cgcggcggta aaaagatttc gatctgaata ttatctccgg
    67381 cctgcactgc aggccggaat gcattgttac accgtccggc gggcaaattc tttaactttg
    67441 aagcccagta ccgccagtgc agcgaagtac gcggcaatcc ccgccagcac gaccgccatt
    67501 aaacgcagta agcgccaggg catggtaccc aatgaccact ccggcatgat atgtaacata
    67561 cctaaaagca cgccagacat caccagtacc gccaccacca gacgcaacag aaacgccatc
    67621 cagccgggtt gcggagtaaa gattttctgc ttacgcaact gccagtaaag cagcgaagca
    67681 ttcagacacg ccgccagacc aatagaaagt gacagcccgg catgtttcaa cggaccaata
    67741 aacgccaggt tcatcaattg cgttaaaatc agcgtaacga tggcaatttt cactggcgtt
    67801 ttaatgtcct ggcgggaata aaagccagga gccaacactt tcactacaat caggccaatc
    67861 aaacccaccg agtaggcaat taacgcccgc tgggtcatca gcgcatcaaa cgcggtaaat
    67921 ttaccgtact ggaacagcga aacggttaac ggacctgaaa gaatgcccaa cgcaaccgca
    67981 ctcggcagcg ccaacaggaa acaaagacgc aatccccagt ccatcaaacg gttgtattca
    68041 tcatgattgc cactggcaaa acttttcgac agcgacggca gcaaaatggt gccaagcgcc
    68101 acgcccagca caccggacgg aaactccatt aagcggtcgg cgtaatacat ccaagacacc
    68161 gaaccggaag caagaaacga ggcaaaaatg gtgttgatga ttaaggagat ctggctcaca
    68221 gagacgccaa ggatcgccgg tcccatctgt ttcaccacgc gcattgctcc ggcatcgtgg
    68281 aagttaatgc gcggcaggac cagcatgccg atcttcttca ggtgcggtag ctgatacacc
    68341 agctgcagga tgccgccgac cgtaacagcc cacgccagcg ccagcaccgg tgggttaaag
    68401 tacggtgcgg caaacagcgc gaaaccaatc atgctgatgt taagcagtgt tggagcaaac
    68461 gccggaatcg agaagcggtt ccacgtattc agaatcgctc ccaccagcga cgccagggag
    68521 atcagcaaga tatagggaaa ggtaatcttt agtagctggc tggtcagggc aaatttgtca
    68581 gctgtgtcag cgaagcctgg cgcggtcacc atgatcaccc acggtgcggc gagcatgcca
    68641 gcgaccgtca caaccgccag cgcgagtgtc agcaggccag aaacataaga gacaaagacc
    68701 cgcgtggcgt cttcaccctg cttactttta tattccgcca gaatcggtac aaatgcctgg
    68761 gaaaatgccc cttcggcaaa gatacggcgt aacaagttag gaagtttaaa agcgacgaaa
    68821 aaggcgtcgg ttgccatccc tgcgccaaag attctggcga caattgcgtc tcgtgcgaag
    68881 ccaagcacac gcgaaaacat ggtcatcgag ctgacggcgg ccagcgattt taataaattc
    68941 atcggtgttc taatccagac ccacgggcaa aacgcctgca aggcaggcgc tgaaagaggc
    69001 gaatagtcta cctggattat ggtgaattgc taccgccaga tgttacaggg tttattcact
    69061 catcgcatcg cgccagatct tgtcaacgat acgttgcgcc agcacggcct gttcgccggc
    69121 ggtttgcgga actgtctgat tttgcacaca ttcaatgaag tgccgcgcac agccgacaaa
    69181 tccacgttgt tcaagagtgc tctgccaacc aggaatcggt ttatgcacta cgccctgccc
    69241 gcgctcctca cgccattcgc gcatatccgt aatgtcgatg agcgcaccgt cagtcacggc
    69301 ctgcacggtt tcacgctgac ttccggcacg gcgatgcata caggtggtga tctgcaaagg
    69361 accagccgaa aaatggtgct cggcaaacag catttcgcca gcgtcgttag tcagtagcgt
    69421 accgccatcc agagaggctt tgccacccga caaccacagc gcggtatcca ccacatgcag
    69481 ataatcatcc agcaacgtga aataaagatc gtgtggcccg acgctattgc tacgatgttt
    69541 atccattctt aacgaggctg cggtggcgag ttgcgttttt aactctccgt agagtggtgc
    69601 gaaacgacgg ttaaaaccga ccatcaacgt cagttttttc cgcgccgcca gttccaccag
    69661 ccgttcagca tcgcgcagat tttctgccag cggtttatcg acacagacat gtacccccgc
    69721 attgagtaac gtactgacca cgtcaaagtg gctggcggtg ctggaatgca caaaaaccgc
    69781 atcgcaactg gcggcaaggc tggataacga atcggcataa ggaatgcgcc agctttcaca
    69841 aattggcagg gctttcgcgc gcgtaggcga ccaggctcct tgtaacatcc agtcagacgc
    69901 tgccgccagc accggtaacc acgctttttg cgcaatgccg cctaatccca caacgccgat
    69961 acgtaatttt ttcactttaa tctcccagat gggccagcaa cgaatcaaga cgctgtttca
    70021 gttctgccac ttcgatttcc agggcttcga cgcgggcctg taaatcacca tcaaccgcgt
    70081 tcgacatatc cgttaccgcc ggctgatcct ctacctcacc actgaaaaga tgcatgtagc
    70141 ggctttcgcg tttacccggt tcgcgggcca gacgcaccac aaaaggacca tcttcgcgat
    70201 ttgccagttg ttccagcgtc gactccactt ccgccatatc gctgaattca tacattcgcg
    70261 cggcgcggct gcgcagttca cctggcgtct gggcaccacg caataacaac gtggtgatta
    70321 acgccacttc cgctgcgctc agtttcagat cgccaaattc tgaattacaa aaacgttgct
    70381 catatttggt gacccgatta ccaaaaccgc tcactgtgcg tagataatga cgtttgacca
    70441 gattatccag ctgttcctgc acttcggatt cactcagatt catgaccggt tcacggttcg
    70501 ttttctgatt acaggccgtg actacaccat tgactgagag cgggtattgc tccggcgtgg
    70561 tcacctgctt ttccagcaag caaccaatca cgcgagcttc aagggcagta agttgatatt
    70621 tcatgagatt ctccttagcg gccgggcgtc cagtctgggg tagttaatgc cgtcagtacg
    70681 tgatcgcgcc attgtccatc aatcaacaga tagtctttcg catagccttc tttttcaaaa
    70741 cccagtcgcg ccagtaaatc accgctgcgt ttattgtgcg gcatataatt agccataatg
    70801 cgatgaatat gttgggttcg ctgcatataa cgaatggctg cggtcagggc ttcaaacatg
    70861 agtcctttgc cctgccattt ttgcccaatc gaataaccga gatagcaggc atgaaaagag
    70921 ccacgaacaa cattggaaaa attggcaacg ccaataatct ctttttcgtc cgggtcgaat
    70981 aagccaaagt agaaagctga accttgttta tgaaattcgt taatcatccc cagcctggcc
    71041 tgccagcctg atggataaca gtggctttcg tcgcgcactg gctcccaggg cttgaggaaa
    71101 tggcgattct ctgcgtaata atccgcaaga cgccaggcat cacgatcatg caccagacgc
    71161 acgaccagtc ggtctgtggt taagcgcact tttggcacgt tactgcgata gccaaacatt
    71221 ctatacctac tccttcccgt aacgtcttta atacgctgta agtattacta tacctgcgca
    71281 accagaccct gtgaaaagtc caaaatgcca ttttctgcat tattcacata tttgataaaa
    71341 actctctatc aaagcgacgt tttgataaaa aaatattgtc cccgaagtga tgggaaaagt
    71401 cttagcggca atgcagaata gaaagtgctc atcttttatt tcccgggagg ggaaatgtcc
    71461 cgcgtatcgc aggcgaggaa cctgggtaaa tatttcctgc tcatcgataa tatgctggtc
    71521 gtgctggggt tctttgttgt cttcccgctg atctctatcc gcttcgttga tcaaatgggc
    71581 tgggccgccg tcatggtcgg tattgctctc ggtctacgcc agtttattca gcaaggtctg
    71641 ggtattttcg gcggcgcaat tgccgaccgc tttggtgcca aaccgatgat tgttaccggt
    71701 atgctgatgc gcgccgccgg attcgccaca atgggtatcg cccacgaacc gtggctattg
    71761 tggttttcat gcctgctctc gggactcggc ggcacgttgt ttgatccgcc gcgttcggcg
    71821 ctggtggtga aactaatccg tccacaacaa cgtggtcgtt ttttctcgct gttgatgatg
    71881 caggacagtg ccggtgcggt cattggcgca ttgttgggga gctggctgtt gcaatacgac
    71941 tttcgcctgg tctgcgccac aggggccgtt ttgtttgtgc tgtgtgcggc gttcaatgcg
    72001 tggttgttac cggcatggaa actctccacc gtacacacgc ccgttcgcga aggcatgacc
    72061 cgcgtgatgc gtgacaagcg ttttgtcacc tatgtcctga cgctggcggg ttactacatg
    72121 ctggctgtac aagtgatgct gatgctgcca attatggtca acgacgtggc tggcgcaccc
    72181 tctgccgtta aatggatgta tgccattgaa gcgtgtctgt cgttaacgtt gctctaccct
    72241 atcgcccgct ggagtgaaaa gcattttcgt ctggaacacc ggttgatggc tgggctgttg
    72301 ataatgtcat taagcatgat gccggtgggc atggtcagcg gcctgcaaca acttttcacc
    72361 ctgatttgtc tgttttatat cgggtcgatc attgccgagc ctgcgcgtga aaccttaagt
    72421 gcttcgctgg cggacgcaag agctcgcggc agctatatgg ggtttagccg tctgggtctg
    72481 gcgattggcg gcgctattgg ttatatcggt ggtggctggc tgtttgacct gggcaaatcg
    72541 gcgcaccagc cagagcttcc gtggatgatg ctgggaatta ttggcatctt cactttcctt
    72601 gcgctgggtt ggcagtttag tcagaaacgc gccgcgcgtc gtttgctgga acgcgacgcc
    72661 tgatttgata ctcccccctg actcctccat actgaaagtg aactgacagc caaatggagg
    72721 agtcacgtga agctatacat ttacgatcac tgcccttact gcctcaaagc ccgcatgatt
    72781 ttcggcctga aaaatatccc cgtcgaatta catgttctgc tcaacgacga cgcagaaaca
    72841 cccacccgga tggtcggtca aaaacaggtt cccattctgc aaaaagatga cagccgctat
    72901 atgccagaaa gcatggatat cgttcactat gtcgataaac tcgacggcaa accgttactg
    72961 accggcaaac gttcccctgc cattgaagag tggctgcgca aggtcaatgg ctacgcaaac
    73021 aaactgctgt tgccgcgttt tgccaaatcg gcatttgatg agttttctac tcccgccgcg
    73081 cgcaaatatt tcgtcgacaa gaaagaggcc agcgcgggta attttgccga cctgctggcc
    73141 cactctgacg gtctgattaa gaatatcagc gatgatttgc gtgcgctgga caaactgatc
    73201 gtcaaaccga acgccgtgaa tggcgaactt tcggaagatg atattcagct atttccgcta
    73261 ctgcgtaatc tgacgctggt agccggaatc aactggccaa gccgcgttgc tgattaccgc
    73321 gataatatgg cgaaacagac acaaatcaat ttgttatcat caatggcgat ttaatcctga
    73381 cccgccgcgg cagcctgtat tcgcgggttg ccgccgtttt cagttttttc tgctggcgag
    73441 tttatcgctg gtgacacatg ctgcgttgtg tcaggatatt gacgcagatt tcatgtcgag
    73501 gactaccatg aacaagtttt tatttgccgc tgcattgatc gtcagtggcc tgctcgttgg
    73561 ctgtaatcaa ctcacccaat acaccatcac cgaacaagaa attaaccagt cgcttgcgaa
    73621 acataataat ttctcaaaag atatcggttt acccggcgtg gctgacgccc atattgttct
    73681 gacaaacttg accagccaaa ttggtcgcga agagccgaat aaggttaccc taaccggaga
    73741 cgccaatctg gacatgaact ccctgttcgg tagtcagaaa gcgaccatga aactgaagct
    73801 gaaagcgctg cccgtgtttg ataaagaaaa aggtgcgatc ttcctgaaag agatggaggt
    73861 ggtcgatgcg acggtacaac cggaaaaaat gcaaacggtg atgcaaacgt tgcttcccta
    73921 tttgaaccag gcattacgca attactttaa ccagcaacct gcttacgtcc tgcgcgaaga
    73981 tggcagtcag ggcgaagcaa tggcgaaaaa actggcgaaa ggcattgaag tgaagccagg
    74041 cgaaattgtc attccgttta cggattaatg acgagggcgc attcgcgccc tttatttttc
    74101 gtgcaaagga aaacgtttcc gcttatcctt tgtgtccggc aaaaacatcc cttcagccgg
    74161 agcatagaga ttaatgactg caccatccca ggtattaaag atccgccgcc cagacgactg
    74221 gcaccttcac ctccgcgatg gcgacatgtt aaaaactgtc gtgccgtata ccagcgaaat
    74281 ttatggacgg gctatcgtaa tgcccaatct ggctccgccc gtgaccacag ttgaggctgc
    74341 cgtggcgtat cgccaacgta ttcttgatgc cgtacctgcc gggcacaatt tcaccccatt
    74401 gatgacctgt tatttaacag attcgctgga tcctaatgag ctggagcgcg gatttaacga
    74461 aggcgtgttc accgctgcaa aactttatcc ggcaaacgca accactaact ccagccacgg
    74521 cgtgacgtca attgacgcaa tcatgccggt acttgagcgc atggaaaaaa tcggtatgcc
    74581 gctactggtg catggtgaag tgacacatgc agatatcgac atttttgatc gtgaagcgcg
    74641 ctttatagaa agcgtgatgg aacctctgcg ccagcgcctg actgcgctga aagtcgtttt
    74701 tgagcacatc accaccaaag atgctgccga ctatgtccgt gacggaaatg aacggctggc
    74761 tgccaccatc actccgcagc atctgatgtt taaccgcaac catatgctgg ttggtggcgt
    74821 gcgtccgcac ctgtattgtt tacccatcct caaacgcaat attcaccaac aggcattgcg
    74881 tgaactggtc gccagcggtt ttaatcgagt attcctcggt acggattctg cgccacatgc
    74941 acgtcatcgc aaagagagca gttgcggctg cgcgggctgc ttcaacgccc caaccgcgct
    75001 gggcagttac gctaccgtct ttgaagagat gaatgctctg cagcactttg aagcattctg
    75061 ttctgtaaac ggcccgcagt tctatggctt gccggtcaac gacacattca tcgaactggt
    75121 acgtgaagag caacaggttg ctgaaagcat cgcactgact gatgacaccc tggtgccatt
    75181 cctcgccggg gaaacggtac gctggtccgt taaacaataa aaatcgtagc ttcctgttgt
    75241 cattaggtta ttttacctgt ataaataacc agtatattca acagggggct attatgcgaa
    75301 ttgaagtcac catagcgaaa acttctccat tgccagctgg ggctattgac gccctggctg
    75361 gcgaactttc ccgccgtatt cagtatgcgt ttcctgataa tgaaggccac gtatcggtac
    75421 gttatgccgc agcgaataat ttatcggtta ttggcgcgac aaaagaagat aaacagcgca
    75481 ttagcgaaat tctccaggaa acgtgggaaa gcgccgatga ctggtttgtc agcgaataat
    75541 atgcagtgat ttttgttttt gccgggtcgc cccggctttt ttattttact tacgaattt
//
LOCUS       DAZGAN020000010        72945 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGAN020000010 DAZGAN020000000
VERSION     DAZGAN020000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 72945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.010"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            138..710
                     /gene="lpfA-O113"
                     /locus_tag="ACHKYO_RS03865"
                     /old_locus_tag="pgaptmp_000773"
     CDS             138..710
                     /gene="lpfA-O113"
                     /locus_tag="ACHKYO_RS03865"
                     /old_locus_tag="pgaptmp_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HGU4427667.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDVVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASLQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            758..1489
                     /gene="lpfB-O113"
                     /locus_tag="ACHKYO_RS03870"
                     /old_locus_tag="pgaptmp_000774"
     CDS             758..1489
                     /gene="lpfB-O113"
                     /locus_tag="ACHKYO_RS03870"
                     /old_locus_tag="pgaptmp_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HGU4427668.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1514..4036
                     /gene="lpfC-O113"
                     /locus_tag="ACHKYO_RS03875"
                     /old_locus_tag="pgaptmp_000775"
     CDS             1514..4036
                     /gene="lpfC-O113"
                     /locus_tag="ACHKYO_RS03875"
                     /old_locus_tag="pgaptmp_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HGU4427669.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            4047..5120
                     /gene="lpfD-O113"
                     /locus_tag="ACHKYO_RS03880"
                     /old_locus_tag="pgaptmp_000776"
     CDS             4047..5120
                     /gene="lpfD-O113"
                     /locus_tag="ACHKYO_RS03880"
                     /old_locus_tag="pgaptmp_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HGU4427670.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            5368..6408
                     /gene="pstS"
                     /locus_tag="ACHKYO_RS03885"
                     /old_locus_tag="pgaptmp_000777"
     CDS             5368..6408
                     /gene="pstS"
                     /locus_tag="ACHKYO_RS03885"
                     /old_locus_tag="pgaptmp_000777"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGU4427671.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6495..7454
                     /gene="pstC"
                     /locus_tag="ACHKYO_RS03890"
                     /old_locus_tag="pgaptmp_000778"
     CDS             6495..7454
                     /gene="pstC"
                     /locus_tag="ACHKYO_RS03890"
                     /old_locus_tag="pgaptmp_000778"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HGU4427672.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7454..8344
                     /gene="pstA"
                     /locus_tag="ACHKYO_RS03895"
                     /old_locus_tag="pgaptmp_000779"
     CDS             7454..8344
                     /gene="pstA"
                     /locus_tag="ACHKYO_RS03895"
                     /old_locus_tag="pgaptmp_000779"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGU4427673.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8435..9208
                     /gene="pstB"
                     /locus_tag="ACHKYO_RS03900"
                     /old_locus_tag="pgaptmp_000780"
     CDS             8435..9208
                     /gene="pstB"
                     /locus_tag="ACHKYO_RS03900"
                     /old_locus_tag="pgaptmp_000780"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGU4427674.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9223..9948
                     /gene="phoU"
                     /locus_tag="ACHKYO_RS03905"
                     /old_locus_tag="pgaptmp_000781"
     CDS             9223..9948
                     /gene="phoU"
                     /locus_tag="ACHKYO_RS03905"
                     /old_locus_tag="pgaptmp_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGU4427675.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            10234..11070
                     /gene="bglG"
                     /locus_tag="ACHKYO_RS03910"
                     /old_locus_tag="pgaptmp_000782"
     CDS             10234..11070
                     /gene="bglG"
                     /locus_tag="ACHKYO_RS03910"
                     /old_locus_tag="pgaptmp_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HGU4427676.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            11203..13080
                     /gene="bglF"
                     /locus_tag="ACHKYO_RS03915"
                     /old_locus_tag="pgaptmp_000783"
     CDS             11203..13080
                     /gene="bglF"
                     /locus_tag="ACHKYO_RS03915"
                     /old_locus_tag="pgaptmp_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HGU4427677.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            13099..14493
                     /gene="bglB"
                     /locus_tag="ACHKYO_RS03920"
                     /old_locus_tag="pgaptmp_000784"
     CDS             13099..14493
                     /gene="bglB"
                     /locus_tag="ACHKYO_RS03920"
                     /old_locus_tag="pgaptmp_000784"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HGU4427678.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLEAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            14579..16195
                     /gene="bglH"
                     /locus_tag="ACHKYO_RS03925"
                     /old_locus_tag="pgaptmp_000785"
     CDS             14579..16195
                     /gene="bglH"
                     /locus_tag="ACHKYO_RS03925"
                     /old_locus_tag="pgaptmp_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HGU4427679.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            16222..17391
                     /gene="yieL"
                     /locus_tag="ACHKYO_RS03930"
                     /old_locus_tag="pgaptmp_000786"
     CDS             16222..17391
                     /gene="yieL"
                     /locus_tag="ACHKYO_RS03930"
                     /old_locus_tag="pgaptmp_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4427680.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVLDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVSKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            17406..18128
                     /gene="yieK"
                     /locus_tag="ACHKYO_RS03935"
                     /old_locus_tag="pgaptmp_000787"
     CDS             17406..18128
                     /gene="yieK"
                     /locus_tag="ACHKYO_RS03935"
                     /old_locus_tag="pgaptmp_000787"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGU4427681.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(18190..18777)
                     /gene="cbrC"
                     /locus_tag="ACHKYO_RS03940"
                     /old_locus_tag="pgaptmp_000788"
     CDS             complement(18190..18777)
                     /gene="cbrC"
                     /locus_tag="ACHKYO_RS03940"
                     /old_locus_tag="pgaptmp_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HGU4427682.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(18826..19299)
                     /locus_tag="ACHKYO_RS03945"
                     /old_locus_tag="pgaptmp_000789"
     CDS             complement(18826..19299)
                     /locus_tag="ACHKYO_RS03945"
                     /old_locus_tag="pgaptmp_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4427683.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            complement(19366..20031)
                     /gene="yieH"
                     /locus_tag="ACHKYO_RS03950"
                     /old_locus_tag="pgaptmp_000790"
     CDS             complement(19366..20031)
                     /gene="yieH"
                     /locus_tag="ACHKYO_RS03950"
                     /old_locus_tag="pgaptmp_000790"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HGU4427684.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            20198..21535
                     /gene="adeP"
                     /locus_tag="ACHKYO_RS03955"
                     /old_locus_tag="pgaptmp_000791"
     CDS             20198..21535
                     /gene="adeP"
                     /locus_tag="ACHKYO_RS03955"
                     /old_locus_tag="pgaptmp_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HGU4427685.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            complement(21589..22155)
                     /gene="yieF"
                     /locus_tag="ACHKYO_RS03960"
                     /old_locus_tag="pgaptmp_000792"
                     /gene_synonym="chrR"
     CDS             complement(21589..22155)
                     /gene="yieF"
                     /locus_tag="ACHKYO_RS03960"
                     /old_locus_tag="pgaptmp_000792"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HGU4427686.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(22177..22926)
                     /gene="yieE"
                     /locus_tag="ACHKYO_RS03965"
                     /old_locus_tag="pgaptmp_000793"
     CDS             complement(22177..22926)
                     /gene="yieE"
                     /locus_tag="ACHKYO_RS03965"
                     /old_locus_tag="pgaptmp_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HGU4427687.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWEFDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            complement(23083..24042)
                     /gene="yidZ"
                     /locus_tag="ACHKYO_RS03970"
                     /old_locus_tag="pgaptmp_000794"
     CDS             complement(23083..24042)
                     /gene="yidZ"
                     /locus_tag="ACHKYO_RS03970"
                     /old_locus_tag="pgaptmp_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HGU4427688.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            complement(24017..25192)
                     /gene="mdtL"
                     /locus_tag="ACHKYO_RS03975"
                     /old_locus_tag="pgaptmp_000795"
     CDS             complement(24017..25192)
                     /gene="mdtL"
                     /locus_tag="ACHKYO_RS03975"
                     /old_locus_tag="pgaptmp_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HGU4427689.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIADDLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(25324..26571)
                     /gene="tnaB"
                     /locus_tag="ACHKYO_RS03980"
                     /old_locus_tag="pgaptmp_000796"
     CDS             complement(25324..26571)
                     /gene="tnaB"
                     /locus_tag="ACHKYO_RS03980"
                     /old_locus_tag="pgaptmp_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HGU4427690.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            complement(26662..28077)
                     /gene="tnaA"
                     /locus_tag="ACHKYO_RS03985"
                     /old_locus_tag="pgaptmp_000797"
     CDS             complement(26662..28077)
                     /gene="tnaA"
                     /locus_tag="ACHKYO_RS03985"
                     /old_locus_tag="pgaptmp_000797"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HGU4427691.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            complement(28298..28372)
                     /gene="tnaC"
                     /locus_tag="ACHKYO_RS03990"
                     /old_locus_tag="pgaptmp_000798"
     CDS             complement(28298..28372)
                     /gene="tnaC"
                     /locus_tag="ACHKYO_RS03990"
                     /old_locus_tag="pgaptmp_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HGU4427692.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            complement(28615..29979)
                     /gene="mnmE"
                     /locus_tag="ACHKYO_RS03995"
                     /old_locus_tag="pgaptmp_000799"
     CDS             complement(28615..29979)
                     /gene="mnmE"
                     /locus_tag="ACHKYO_RS03995"
                     /old_locus_tag="pgaptmp_000799"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGU4427693.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(30085..31731)
                     /gene="yidC"
                     /locus_tag="ACHKYO_RS04000"
                     /old_locus_tag="pgaptmp_000800"
     CDS             complement(30085..31731)
                     /gene="yidC"
                     /locus_tag="ACHKYO_RS04000"
                     /old_locus_tag="pgaptmp_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGU4427694.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(31734..31991)
                     /gene="yidD"
                     /locus_tag="ACHKYO_RS04005"
                     /old_locus_tag="pgaptmp_000801"
     CDS             complement(31734..31991)
                     /gene="yidD"
                     /locus_tag="ACHKYO_RS04005"
                     /old_locus_tag="pgaptmp_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGU4427695.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(31955..32314)
                     /gene="rnpA"
                     /locus_tag="ACHKYO_RS04010"
                     /old_locus_tag="pgaptmp_000802"
     CDS             complement(31955..32314)
                     /gene="rnpA"
                     /locus_tag="ACHKYO_RS04010"
                     /old_locus_tag="pgaptmp_000802"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGU4427696.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(32331..32471)
                     /gene="rpmH"
                     /locus_tag="ACHKYO_RS04015"
                     /old_locus_tag="pgaptmp_000803"
     CDS             complement(32331..32471)
                     /gene="rpmH"
                     /locus_tag="ACHKYO_RS04015"
                     /old_locus_tag="pgaptmp_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGU4427697.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(32701..32781)
                     /locus_tag="ACHKYO_RS04020"
                     /old_locus_tag="pgaptmp_000804"
     CDS             complement(32701..32781)
                     /locus_tag="ACHKYO_RS04020"
                     /old_locus_tag="pgaptmp_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4427698.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            33078..34481
                     /gene="dnaA"
                     /locus_tag="ACHKYO_RS04025"
                     /old_locus_tag="pgaptmp_000805"
     CDS             33078..34481
                     /gene="dnaA"
                     /locus_tag="ACHKYO_RS04025"
                     /old_locus_tag="pgaptmp_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGU4427699.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            34486..35586
                     /gene="dnaN"
                     /locus_tag="ACHKYO_RS04030"
                     /old_locus_tag="pgaptmp_000806"
     CDS             34486..35586
                     /gene="dnaN"
                     /locus_tag="ACHKYO_RS04030"
                     /old_locus_tag="pgaptmp_000806"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGU4427700.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            35586..36659
                     /gene="recF"
                     /locus_tag="ACHKYO_RS04035"
                     /old_locus_tag="pgaptmp_000807"
     CDS             35586..36659
                     /gene="recF"
                     /locus_tag="ACHKYO_RS04035"
                     /old_locus_tag="pgaptmp_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGU4427701.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            36688..39102
                     /gene="gyrB"
                     /locus_tag="ACHKYO_RS04040"
                     /old_locus_tag="pgaptmp_000808"
     CDS             36688..39102
                     /gene="gyrB"
                     /locus_tag="ACHKYO_RS04040"
                     /old_locus_tag="pgaptmp_000808"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGU4427702.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            39342..39740
                     /gene="yidB"
                     /locus_tag="ACHKYO_RS04045"
                     /old_locus_tag="pgaptmp_000809"
     CDS             39342..39740
                     /gene="yidB"
                     /locus_tag="ACHKYO_RS04045"
                     /old_locus_tag="pgaptmp_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HGU4427703.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            39855..40667
                     /gene="yidA"
                     /locus_tag="ACHKYO_RS04050"
                     /old_locus_tag="pgaptmp_000810"
     CDS             39855..40667
                     /gene="yidA"
                     /locus_tag="ACHKYO_RS04050"
                     /old_locus_tag="pgaptmp_000810"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGU4427704.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(40713..41369)
                     /gene="yidX"
                     /locus_tag="ACHKYO_RS04055"
                     /old_locus_tag="pgaptmp_000811"
     CDS             complement(40713..41369)
                     /gene="yidX"
                     /locus_tag="ACHKYO_RS04055"
                     /old_locus_tag="pgaptmp_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HGU4427705.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            41647..42336
                     /gene="dgoR"
                     /locus_tag="ACHKYO_RS04060"
                     /old_locus_tag="pgaptmp_000812"
     CDS             41647..42336
                     /gene="dgoR"
                     /locus_tag="ACHKYO_RS04060"
                     /old_locus_tag="pgaptmp_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HGU4427706.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            42333..43211
                     /gene="dgoK"
                     /locus_tag="ACHKYO_RS04065"
                     /old_locus_tag="pgaptmp_000813"
     CDS             42333..43211
                     /gene="dgoK"
                     /locus_tag="ACHKYO_RS04065"
                     /old_locus_tag="pgaptmp_000813"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HGU4427707.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            43195..43812
                     /gene="dgoA"
                     /locus_tag="ACHKYO_RS04070"
                     /old_locus_tag="pgaptmp_000814"
     CDS             43195..43812
                     /gene="dgoA"
                     /locus_tag="ACHKYO_RS04070"
                     /old_locus_tag="pgaptmp_000814"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HGU4427708.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            43809..44957
                     /gene="dgoD"
                     /locus_tag="ACHKYO_RS04075"
                     /old_locus_tag="pgaptmp_000815"
     CDS             43809..44957
                     /gene="dgoD"
                     /locus_tag="ACHKYO_RS04075"
                     /old_locus_tag="pgaptmp_000815"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HGU4427709.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHVDNSVAEW"
     gene            45032..46369
                     /gene="dgoT"
                     /locus_tag="ACHKYO_RS04080"
                     /old_locus_tag="pgaptmp_000816"
     CDS             45032..46369
                     /gene="dgoT"
                     /locus_tag="ACHKYO_RS04080"
                     /old_locus_tag="pgaptmp_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4427710.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYYIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(46366..47430)
                     /gene="cbrA"
                     /locus_tag="ACHKYO_RS04085"
                     /old_locus_tag="pgaptmp_000817"
     CDS             complement(46366..47430)
                     /gene="cbrA"
                     /locus_tag="ACHKYO_RS04085"
                     /old_locus_tag="pgaptmp_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HGU4427711.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            47531..48745
                     /gene="yidR"
                     /locus_tag="ACHKYO_RS04090"
                     /old_locus_tag="pgaptmp_000818"
     CDS             47531..48745
                     /gene="yidR"
                     /locus_tag="ACHKYO_RS04090"
                     /old_locus_tag="pgaptmp_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HGU4427712.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGLVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(48747..49079)
                     /gene="yidQ"
                     /locus_tag="ACHKYO_RS04095"
                     /old_locus_tag="pgaptmp_000819"
     CDS             complement(48747..49079)
                     /gene="yidQ"
                     /locus_tag="ACHKYO_RS04095"
                     /old_locus_tag="pgaptmp_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU4427713.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    49319..49391
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            49385..49798
                     /gene="ibpA"
                     /locus_tag="ACHKYO_RS04100"
                     /old_locus_tag="pgaptmp_000820"
     CDS             49385..49798
                     /gene="ibpA"
                     /locus_tag="ACHKYO_RS04100"
                     /old_locus_tag="pgaptmp_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HGU4427714.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            49910..50338
                     /gene="ibpB"
                     /locus_tag="ACHKYO_RS04105"
                     /old_locus_tag="pgaptmp_000821"
     CDS             49910..50338
                     /gene="ibpB"
                     /locus_tag="ACHKYO_RS04105"
                     /old_locus_tag="pgaptmp_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HGU4427715.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            50535..52196
                     /gene="yidE"
                     /locus_tag="ACHKYO_RS04110"
                     /old_locus_tag="pgaptmp_000822"
     CDS             50535..52196
                     /gene="yidE"
                     /locus_tag="ACHKYO_RS04110"
                     /old_locus_tag="pgaptmp_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HGU4427716.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(52193..52909)
                     /gene="yidP"
                     /locus_tag="ACHKYO_RS04115"
                     /old_locus_tag="pgaptmp_000823"
     CDS             complement(52193..52909)
                     /gene="yidP"
                     /locus_tag="ACHKYO_RS04115"
                     /old_locus_tag="pgaptmp_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4427717.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPVIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            53205..54821
                     /locus_tag="ACHKYO_RS04120"
                     /old_locus_tag="pgaptmp_000824"
     CDS             53205..54821
                     /locus_tag="ACHKYO_RS04120"
                     /old_locus_tag="pgaptmp_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4427718.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEAFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            54821..55635
                     /locus_tag="ACHKYO_RS04125"
                     /old_locus_tag="pgaptmp_000825"
                     /pseudo
     CDS             54821..55635
                     /locus_tag="ACHKYO_RS04125"
                     /old_locus_tag="pgaptmp_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(55632..56525)
                     /gene="yidL"
                     /locus_tag="ACHKYO_RS04130"
                     /old_locus_tag="pgaptmp_000826"
     CDS             complement(55632..56525)
                     /gene="yidL"
                     /locus_tag="ACHKYO_RS04130"
                     /old_locus_tag="pgaptmp_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4427719.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLTPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            56692..58407
                     /gene="yidK"
                     /locus_tag="ACHKYO_RS04135"
                     /old_locus_tag="pgaptmp_000827"
     CDS             56692..58407
                     /gene="yidK"
                     /locus_tag="ACHKYO_RS04135"
                     /old_locus_tag="pgaptmp_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HGU4427720.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGSPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            58404..59897
                     /gene="yidJ"
                     /locus_tag="ACHKYO_RS04140"
                     /old_locus_tag="pgaptmp_000828"
     CDS             58404..59897
                     /gene="yidJ"
                     /locus_tag="ACHKYO_RS04140"
                     /old_locus_tag="pgaptmp_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4427721.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGTHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(59944..60393)
                     /gene="yidI"
                     /locus_tag="ACHKYO_RS04145"
                     /old_locus_tag="pgaptmp_000829"
     CDS             complement(59944..60393)
                     /gene="yidI"
                     /locus_tag="ACHKYO_RS04145"
                     /old_locus_tag="pgaptmp_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HGU4427722.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            60503..60850
                     /gene="yidH"
                     /locus_tag="ACHKYO_RS04150"
                     /old_locus_tag="pgaptmp_000830"
     CDS             60503..60850
                     /gene="yidH"
                     /locus_tag="ACHKYO_RS04150"
                     /old_locus_tag="pgaptmp_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HGU4427723.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            60840..61202
                     /gene="yidG"
                     /locus_tag="ACHKYO_RS04155"
                     /old_locus_tag="pgaptmp_000831"
     CDS             60840..61202
                     /gene="yidG"
                     /locus_tag="ACHKYO_RS04155"
                     /old_locus_tag="pgaptmp_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HGU4427724.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            61199..61696
                     /gene="yidF"
                     /locus_tag="ACHKYO_RS04160"
                     /old_locus_tag="pgaptmp_000832"
     CDS             61199..61696
                     /gene="yidF"
                     /locus_tag="ACHKYO_RS04160"
                     /old_locus_tag="pgaptmp_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGU4427725.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRAVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(61704..62888)
                     /gene="emrD"
                     /locus_tag="ACHKYO_RS04165"
                     /old_locus_tag="pgaptmp_000833"
     CDS             complement(61704..62888)
                     /gene="emrD"
                     /locus_tag="ACHKYO_RS04165"
                     /old_locus_tag="pgaptmp_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HGU4427726.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSDQGQ
                     PV"
     gene            62970..63044
                     /gene="ysdE"
                     /locus_tag="ACHKYO_RS04170"
                     /old_locus_tag="pgaptmp_000834"
     CDS             62970..63044
                     /gene="ysdE"
                     /locus_tag="ACHKYO_RS04170"
                     /old_locus_tag="pgaptmp_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HGU4427727.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(63307..63396)
                     /gene="tisB"
                     /locus_tag="ACHKYO_RS04175"
                     /old_locus_tag="pgaptmp_000835"
     CDS             complement(63307..63396)
                     /gene="tisB"
                     /locus_tag="ACHKYO_RS04175"
                     /old_locus_tag="pgaptmp_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HGU4427728.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            63961..64059
                     /gene="ivbL"
                     /locus_tag="ACHKYO_RS04180"
                     /old_locus_tag="pgaptmp_000836"
     CDS             63961..64059
                     /gene="ivbL"
                     /locus_tag="ACHKYO_RS04180"
                     /old_locus_tag="pgaptmp_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HGU4427729.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            64165..65853
                     /gene="ilvB"
                     /locus_tag="ACHKYO_RS04185"
                     /old_locus_tag="pgaptmp_000837"
     CDS             64165..65853
                     /gene="ilvB"
                     /locus_tag="ACHKYO_RS04185"
                     /old_locus_tag="pgaptmp_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGU4427730.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            65857..66147
                     /gene="ilvN"
                     /locus_tag="ACHKYO_RS04190"
                     /old_locus_tag="pgaptmp_000838"
     CDS             65857..66147
                     /gene="ilvN"
                     /locus_tag="ACHKYO_RS04190"
                     /old_locus_tag="pgaptmp_000838"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU4427731.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            66222..66812
                     /gene="uhpA"
                     /locus_tag="ACHKYO_RS04195"
                     /old_locus_tag="pgaptmp_000839"
     CDS             66222..66812
                     /gene="uhpA"
                     /locus_tag="ACHKYO_RS04195"
                     /old_locus_tag="pgaptmp_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HGU4427732.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            66812..68314
                     /gene="uhpB"
                     /locus_tag="ACHKYO_RS04200"
                     /old_locus_tag="pgaptmp_000840"
     CDS             66812..68314
                     /gene="uhpB"
                     /locus_tag="ACHKYO_RS04200"
                     /old_locus_tag="pgaptmp_000840"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HGU4427733.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            68324..69643
                     /gene="uhpC"
                     /locus_tag="ACHKYO_RS04205"
                     /old_locus_tag="pgaptmp_000841"
     CDS             68324..69643
                     /gene="uhpC"
                     /locus_tag="ACHKYO_RS04205"
                     /old_locus_tag="pgaptmp_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGU4427734.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            69781..71172
                     /gene="uhpT"
                     /locus_tag="ACHKYO_RS04210"
                     /old_locus_tag="pgaptmp_000842"
     CDS             69781..71172
                     /gene="uhpT"
                     /locus_tag="ACHKYO_RS04210"
                     /old_locus_tag="pgaptmp_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HGU4427735.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(71217..>72945)
                     /gene="adeD"
                     /locus_tag="ACHKYO_RS04215"
                     /old_locus_tag="pgaptmp_000843"
     CDS             complement(71217..>72945)
                     /gene="adeD"
                     /locus_tag="ACHKYO_RS04215"
                     /old_locus_tag="pgaptmp_000843"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HGU4427736.1"
                     /translation="RAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGR
                     YIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTV
                     ICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLA
                     WRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGI
                     ENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPW
                     EIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLS
                     DARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPG
                     KRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGFGLN
                     EGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGL
                     MSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKF
                     AFTTLEVTE"
BASE COUNT        17670 a        18059 c        19208 g        18008 t
ORIGIN      
        1 tattttattt ttgcgtaatt aatattattt ttagataaag ccgtctcttt ggtgtactat
       61 tcctttgaat caaaaataca cttattaata taagtgatga ttcatggtaa aggatatatt
      121 atatcaatgg agtttttatg aagcgtaata ttataggcgg tgcattcact ctggcatctc
      181 taatgctggc cgggcatgca ctggcagaag atgttgttgt tcacttcgtc ggtgaaattg
      241 tcgacactac ttgtgaagtt acctccgata cagccgatca aattgtccca ctgggtaaag
      301 tcagtaaaaa tgcattttca ggtgtaggta gtctggcgtc gctacagaag ttcagtatta
      361 agcttgaaaa ttgcccggca acgtacactc aagcagccgt tcgttttgat ggtacagaag
      421 cgcctggcgg cgacggcgac ctgaaagtgg gtacgccgct tacagcaggc aaccctggtg
      481 attttaccgg tacaggacaa gcgattgcgg caaccggcgt tggtattcgt atttttaacc
      541 agtccgataa ttcgcaggtt aaactttata acgactctgc ttataccgct atcgatgctg
      601 aaggcaaggc tgaaatgaag tttattgcac gctatgtggc aaccaatgcg accgtaacgg
      661 ctggtacggc gaacgctgat tcacaattta ctgtcgaata taagaaataa tttatcaagg
      721 gcctttttat tcagttggct ttggccaggg agtcgttatg tccagaaaaa gttgtttaat
      781 caggctcttt aaaacaacag tttcatgtaa gttatttacc gcgattatac tcagcgtttt
      841 tttaggccaa ccagcattaa cttatgcagg cgttgtcatt ggtggtacgc gggtggttta
      901 cttaagtaat aatgctgata aatctatttc tgtatttagc aaagaagaaa aaatacctta
      961 tctcatccag gcttgggtag atccatttaa taaagaggat aaaagcaaag cgccgtttac
     1021 cgttattccg ccagtatcac gattagaacc ttcgcaagaa aaaatattac gcattattca
     1081 caccaaaggt gtgtcattgc ctgatgatcg cgagtcagtc ttctggctga atattaaaaa
     1141 tattccgcca tcagcgagca acaaagcgac gaactcgctg gaaatagcag taaaaacgcg
     1201 tatcaagctg ttctggcgac cagcaaatat ccgccttatt cccgaagatg ctgcgccgaa
     1261 agtgaaatgg cgccgggaag ggcgaaatct gatagcagag aatcccaacc caattcatat
     1321 cagtgtgatg gatgtcatcg ttgatggcca tgatgtgccc ctcaatatga tccgtccttt
     1381 tgaaacgctc acgttacctt tacctgctaa ttctgcggga ggccaaatga cctggcgatt
     1441 tattaatgat tacggcgcag tcagtgaccc gataaaaatg acgctgtaag caagggataa
     1501 ggataggcgt attatgatga cgaccagaat agtggttggc ctcacggcag ggacgtgtct
     1561 gattttctcg caaaacctga tggccgaggt cagtgtattc aatccggcgc ttctggaaat
     1621 cgaccatcaa tccggagtcg atattcgcca gtttaatcgg gcaaacctga tgcccccagg
     1681 tgtttatagc gttgatattt ttatcaacgg taaaatgttt gaacgtcagg atgtgacatt
     1741 tgttcaggat aatccagatg ctgatctgca cgcatgcttt attgccatta aaaaaacact
     1801 gtcctccttt ggcataaaag ttgatgcgct caaatcgttc aatgatgtgg atgagacggt
     1861 ttgcctcgat cctgctccac gtattgaagg ctcatcctgg cagtttgaca gtgataaatt
     1921 gcagctgaat atatccattc cccaaatcta catggacgcg atggcttatg attacatcag
     1981 ccccacgcgt tgggatgagg ggattaacgc gctcaccatc aactacgatt tttctggttc
     2041 acatacacta cgttcagatt atggttcaca agagacagat accagttatc tcaatctgcg
     2101 caatggactg aatattggac cgtggcggct acgtaattac agtactttaa acaccagcga
     2161 tggccgtgcg gaatacaact ccattagtac ctggatacag cgcgatattg ccgcgttaag
     2221 aagccagatt atgattggtg atacgtggac ggcgagcgat attttcgaca gtacgcaaat
     2281 tcgcggcgcg cgtttgtata ctgataacga tatgctaccc gccagccaga atggctttgc
     2341 tcctgtggtt cgtgggattg caaagtccaa cgccaccgtc atcattcggc agaatggcta
     2401 cgtgatttat cagtctgccg ttccacaagg tgcttttgag atcaccgatc tcaacaccgc
     2461 cagtacaggt ggcgatttgg acgtaaccat caaagaagaa gacggtagcg aacaacgatt
     2521 cacccaacct tatgcttcat tggcgattct taaacgtgaa ggtcagacag atgttgatgt
     2581 cagcgtgggt gaattgcgcg atgaagacgg atttacaccg gacgtccttc aggcgcaaat
     2641 acttcatggt ttttcccacg ggatcacttt atatggaggt atgcaggctg ctgaaaatta
     2701 tggttctgca gctctgggtg tcggtaaaga tcttggcgct ttgggcgcaa tttctttcga
     2761 tgtgacacat gctcgtgcga attttagcca tgatgataca gaaacgggtc agtcatatcg
     2821 ctttctctat tcaaaacgat ttgacgacac agacactagc ttgcgcctgg ttggctatcg
     2881 ttactccacc gagggctact ataccctcaa tgagtgggca tcgcggcgca acagccctga
     2941 agacttttgg gaaacaggta accgacgtag tcgcgtggag ggaacgctaa cgcagtcgtt
     3001 ggggagagat tatggcaatt tatacctgac attaagccgg caacaatact ggcataccga
     3061 tgatgtcgaa cgattaatgc aatttggcta cagcagtagc tggaagcgtc tctcgtggaa
     3121 cgtctcctgg agttattcca atactgccag acaggggacg gggaacaacc atgccagtga
     3181 taacaccagt gagcagatct acatgctctc tttatctgtt cctttatcgg gctggtgggg
     3241 taatagttac gccacctatt ctgtttcgca aaacgataat tccggtagct cacatcaact
     3301 cggactcagc ggtacggcgc tggaaagaaa taacctttca tggaatttaa tgcagtccta
     3361 taacagtcat gatgatgagg ttggcggtaa tatgtccctg acctatgatg gctcttatgg
     3421 cacggtgaac ggcagctata actacagcca aaattcccag aggctgaatt atggtatcag
     3481 agggggaatt ctggcacaca gcgaaggggt aacgttaagt caggagttag gtgaaactat
     3541 tgctcttgtt aaagcacctg gggccgccgg gttagaaata gataatatgc gcggtgctgc
     3601 gacggactgg cgcggctata cggtcaagac acagctaaac ccttatgatg aaaatcgggt
     3661 agcaatcagc gataactatt tctcgaagtc gaatatagaa cttgataata ccgtcgttac
     3721 gatggttccc acgcgtggtg cagtggttaa agcggagttt gtgactcatg tgggttatcg
     3781 cgttctcttc agagtgttaa atgcaaatgg taaaccggta ccttttggag ccattgctgc
     3841 gatacaagat gcaagtttgg cagattcagg aattgtcggt gaccgtggcg aactttatct
     3901 ttctggtcta ccagaaaaag gacaggttac gttatcctgg ggagaaaacg cctcaacaaa
     3961 atgcatcttc aattattcac tttcgacacc agaaagtgag agcggattaa ttgaacaggg
     4021 tgtgacatgt cattaaaagg atttttatga acaagtatat caaacagtgg tgctttgctg
     4081 tgtttatgct ctcgttaagt agtgtagcac ttgcagctcc taaaggtatc tgtacccctg
     4141 ataatggcgt atttcacagt acgcttgatt tctcgggata tcttattacc gcgaacgaaa
     4201 ataaggttgg aacgacattt aacaccactg taactaatgg atcctcatat cctggacgct
     4261 gtcattgtga tactggtaac gtaggagaat tcccctatat ctattatacg tcaaaaataa
     4321 accaggcatt aacttatgct ggggttcact ctaatattaa ttattatgat cttaatccga
     4381 accttgatgt tgggattgct atagatattc ttggggttgg gtatgtgaat gcaccttttg
     4441 aatatcatgc taacaacccg tctggtaata caaagtacaa ttgcaaccgc attgaacctt
     4501 taagtatatc tagtggggct aaagcgatag tatattttta tatcaagaag acatttgcag
     4561 gaaaattgat tattcctgaa acaaagatag tgacattgta tggaacaatt agccgtgaca
     4621 ccccggtgga ttactcacag ccgatggctg atgtttatat tcggggcgat attacagctc
     4681 cgcaaagttg cgaaataaat aatttacagc cagtttattt tgattttaaa gaaataccag
     4741 ctgcagattt ttcatctgtt gttggaagtg cggtaacaac acataaaatt actaaaacgg
     4801 tcactattga gtgtgaaaac ctggggatac tgaataccga tgatatcagt acgtcttttt
     4861 atgctaccga acccaatacg gacaattcaa tggtcgtaac ttcaaactct aacgtgggga
     4921 taaaaattta cgacaaaaat aataaggaaa tcaaagtgaa cggtggtgag ttgccaacag
     4981 acatgggtaa atcaacagtg tatggtgaaa aatcaggtag tgtaactttt tcagctgctc
     5041 ctgcgagcct tactggagct cgcccagccc cggggcaatt tactgcaaca gcgacgataa
     5101 ctgttgagat tgtgcgctaa taggtctgac aacgattgtt catcacatcg ttgtcatctc
     5161 acccatttct ttatcacttt taaatgcatc atgacaaaaa gatgacatgt gggattttca
     5221 tcttcacatt taaaatcagt aagttatttg gtaattcctt tctgtcataa aaatgtcata
     5281 ttccttacat ataactgtca cctgtttgtc ctattttgct tctcgtagcc aacaaacaat
     5341 gctttatgaa tcctcccagg agacattatg aaagttatgc gtaccaccgt cgcaactgtt
     5401 gtcgccgcga ccttatcgat gagcgctttc tctgtgtttg cagaagcaag cctgacaggt
     5461 gcaggtgcaa ccttccctgc gccggtgtat gccaaatggg ctgacactta ccagaaagaa
     5521 accggtaata aagttaacta ccagggtatc ggttcttccg gtggcgtaaa acagattatc
     5581 gctaataccg ttgattttgg tgcctctgac gcgccgctgt ctgacgaaaa actggctcag
     5641 gaaggtctgt tccagttccc gaccgtgatt ggcggcgtgg tgctggcggt taacattcca
     5701 gggctgaagt ctggcgaact ggtgctggat ggtaaaaccc tcggcgacat ctacctgggc
     5761 aaaatcaaga agtgggatga tgaagccatc gccaaactga atccgggtct gaaactgcct
     5821 tcacaaaaca ttgctgtagt acgccgcgca gatggctccg ggacttcctt cgtcttcacc
     5881 agctacctgg cgaaagtgaa cgaagagtgg aaaaacaacg ttggtactgg ctctaccgta
     5941 aaatggccga tcggtctggg cggtaaaggt aacgacggta tcgccgcgtt cgttcagcgt
     6001 ctgccgggtg caattggtta tgttgaatat gcttacgcga agcagaacaa cctggcgtac
     6061 accaaactga tctccgctga tggtaaaccg gttagtccga ccgaagaaaa cttcgctaat
     6121 gcagcaaaag gtgcagactg gagcaaaacc ttcgctcagg atctgaccaa ccagaaaggc
     6181 gaagatgcat ggcctattac ctctaccacg ttcattctga tccacaaaga tcagaagaaa
     6241 ccagaacaag gcacagaagt gctgaagttc ttcgactggg cttacaaaac cggggctaaa
     6301 caggcgaacg acctggatta cgccagcctg ccggatagcg tagttgaaca ggttcgcgct
     6361 gcgtggaaga ccaatattaa agacagtagc ggtaagccgc tgtactaata aaactccagg
     6421 ccgggtacgg tgttttacgc cgcatccggc attacaaaat gactttgtaa acgcgtttaa
     6481 ctgaagagta acttatggct gcaaccaagc ctgcttttaa cccaccgggt aaaaagggcg
     6541 acataatttt cagcgtgctg gtaaaactgg cggcgctgat tgtgctattg atgttgggtg
     6601 gcattattgt ctctctgatc atctcctcct ggccgagcat tcagaaattt ggtctggctt
     6661 tcctatggac caaagagtgg gatgcaccga acgatatcta cggggcgctg gtgccgatct
     6721 acggtacgtt ggtgacttcg tttatcgcgc tgctgatcgc cgtcccggtg agtttcggta
     6781 tcgccctgtt cctgactgag cttgcgcctg gctggctgaa acgcccgctg ggtatcgcca
     6841 ttgagctgct ggcagccatt ccaagtatcg tttacggcat gtggggcctg tttatctttg
     6901 cgccgctgtt cgccgtttac tttcaggagc cggtcggcaa tatcatgtcg aatatcccga
     6961 ttgttggcgc gctgttctct ggccccgcat ttggtatcgg tatcctcgcg gcaggcgtga
     7021 tcctcgccat catgattatt ccgtacattg cggcggtaat gcgtgatgtg ttcgaacaaa
     7081 ccccggtgat gatgaaagag tcggcctacg gtattggctg caccacctgg gaagttatct
     7141 ggcgtatcgt tcttccgttc accaaaaatg gtgttatcgg cggcatcatg ctggggctgg
     7201 gccgcgcgct cggtgaaacc atggcggtga cctttatcat cggtaacacc taccagctcg
     7261 acagcgcctc gctgtatatg ccgggcaaca gtatcacctc tgcgctggcg aacgaatttg
     7321 cggaagcgga atccggtctg cacgttgccg cactgatgga actgggcctg atcctgtttg
     7381 tgattacctt catcgtcctc gccgcatcga agtttatgat tatgcgcctg gctaagaatg
     7441 agggggcacg ctaatggcta tggttgaaat gcaaaccact gcggcgctgg ctgaatctcg
     7501 ccgcaaaatg caggcgcgtc gccgcctcaa aaaccgtatt gcgctgacgc tctcgatggc
     7561 gacgatggcc ttcggcctgt tctggctgat ctggatttta atgtccacca tcactcgcgg
     7621 tatcgacggt atgtcgctgg cgctgttcac tgaaatgacg ccgccgccca atacggaagg
     7681 tggtggtctg gcgaacgccc tggcgggtag cgggctgtta attttgtggg ccacggtatt
     7741 cggtacgccg ctgggcatta tggcggggat ttatctggcg gaatatggtc gtaaatcctg
     7801 gctggcagaa gtgattcgct tcattaacga cattctgctc tccgcgccgt cgattgtggt
     7861 tggtctgttt gtttacacca tcgtggtggc gcagatggag cacttctctg gctgggcggg
     7921 tgtaattgcg ctggcgctgt tgcaggtgcc gattgttatc cgcaccaccg agaacatgct
     7981 gaaactggtg ccggacagcc tgcgtgaagc ggcttatgcg ctgggtacac cgaagtggaa
     8041 aatgatctcc gcgattacgc tgaaagcgtc ggtgtccggg atcatgaccg gtatcctgct
     8101 ggcgattgcc cgtattgccg gtgaaaccgc gccgctgctg tttaccgcgc tctccaacca
     8161 gttctggagc acggacatga tgcagccgat cgccaacctg ccggtgacga tctttaagtt
     8221 tgcgatgagc ccgtttgcag aatggcagca actggcctgg gccggggtgt tgattattac
     8281 cctgtgcgta ctgctgctga acattctggc gcgcgttgtt tttgcgaaga ataaacacgg
     8341 ttaacacttt gccggatgcg gcgtgaacgc cttatccggc ctacggtaag cctgattcgc
     8401 gaagtgcatc aggcacgatg aggaaaagat tgcaatgagt atggttgaaa ctgccccgag
     8461 taaaattcag gttcgtaatt tgaacttcta ctacggcaaa ttccatgccc tgaaaaacat
     8521 caacctggat atcgctaaaa accaggtaac ggcgtttatc gggccgtccg gctgcggtaa
     8581 atcgacgctg ctgcgtacct tcaacaaaat gtttgaactg tacccggagc agcgtgcgga
     8641 aggtgaaatt ctgcttgatg gcgacaacat cctgaccaac tctcaggata tcgccctgct
     8701 gcgtgcgaaa gtgggcatgg tgttccagaa accgacgccg tttccgatgt ccatctacga
     8761 caacatcgct tttggcgttc gtctgtttga gaagctctcc cgtgccgaca tggacgagcg
     8821 cgtgcagtgg gcattgacca aagccgcatt gtggaacgaa accaaagata aattgcacca
     8881 gagcggttac tctctctctg gtggtcagca acagcgtctg tgtattgcgc gtggtatcgc
     8941 cattcgcccg gaagtgctgc tgctcgacga accgtgttcg gcgctcgacc ctatctctac
     9001 cgggcgtatt gaagagctga tcaccgaact gaagcaggat tacaccgtgg tgatcgtcac
     9061 ccacaacatg cagcaggctg cgcgttgttc cgaccacacg gcgtttatgt acctgggcga
     9121 attgattgag ttcagcaaca cggacgatct gttcaccaag ccagcgaaga aacaaacaga
     9181 agactacatc accggtcgtt acggttgatt caggagtgcg ttatggacag tctcaatctt
     9241 aataaacata tttccggcca gttcaacgcc gaactggaaa gtatccgcac gcaggtgatg
     9301 accatgggcg gcatggtgga gcagcagctt tctgatgcaa tcaccgcgat gcataaccag
     9361 gacagcgatc tggcgaagcg cgtgatcgaa ggcgacaaga acgtcaacat gatggaagtg
     9421 gcgatcgatg aagcctgcgt gcgcattatc gccaaacgtc agccgacggc gagcgacctg
     9481 cgactggtta tggtgatcag taaaaccatt gccgagctgg agcgtattgg cgacgtggcg
     9541 gacaaaatct gccgtactgc gctggagaaa ttctcccagc agcatcagcc gttgctggta
     9601 agtctggagt cgctgggccg tcataccatc cagatgctgc acgacgtgct ggacgcgttc
     9661 gcgcggatgg acattgacga agcggtacgt atttatcgtg aagataaaaa agtcgatcag
     9721 gaatacgaag gtattgttcg tcaactgatg acctacatga tggaagattc gcgtaccatt
     9781 ccgagcgtac ttactgcgct gttctgcgcg cgttctatcg aacgtattgg cgaccgctgc
     9841 cagaatattt gtgagtttat cttctactac gtgaaggggc aggatttccg tcatgtaggc
     9901 ggtgatgagc tggataaact gctggcggga aaagatagcg acaaataatt caccagacaa
     9961 atcccaataa cttaattatt gggatttgtt atatataact ttataaattc ctaaaattac
    10021 acaaagttaa taactgcgag catggtcata tttttatcaa tagcgcattg ctattttctc
    10081 tgcacgcaat taaattaatt tccgaacctg gatgttcgtt ataaaaacca ttaataaatg
    10141 actggattgt tactgcattc gcaggcaaaa cctgacataa ccagagaata ctggtgaagt
    10201 cgggtttttt tgtttataaa aaaggtcctt gctatgaaca tgcaaatcac caaaattctc
    10261 aacaataatg ttgtggtggt tattgatgat caacagcggg aaaaagtcgt catggggcga
    10321 ggaattggct ttcaaaaacg cgctggcgaa agaattaact caagtggaat agaaaaagag
    10381 tatgccttga gcagtcatga actgaacggg cgattaagcg aactcttaag tcatattcct
    10441 cttgaggtga tggcaacctg tgatcgtatt atctctttag cgcaggagcg cttgggaaaa
    10501 ttacaggaca gtatttatat ctcgctaact gaccattgcc agtttgcgat taaacgcttt
    10561 cagcaaaacg tgttgctgcc caacccgttg ctgtgggata tccagcggct ttacccgaaa
    10621 gagttccagc taggggaaga agcgttaacc attattgata aacggttggg cgtgcagtta
    10681 ccgaaagatg aagtgggctt tattgccatg catctggtca gtgcccaaat gagcggaaat
    10741 atggaggatg ttgcaggtgt cacgcagtta atgcgcgaaa tgctgcaatt aataaaattt
    10801 cagttcagcc ttaattacca ggaagaaagc ttgagttatc agcgactggt tacacatctg
    10861 aagtttttat cctggcgtat tcttgaacat gcttcaatta acgatagtga tgaatcatta
    10921 caacaagcag taaaacaaaa ttacccgcaa gcatggcaat gtgcggaacg gatcgccatt
    10981 tttattggtt tgcagtatca acgtaaaatt tcacccgcag agattatgtt tttagccata
    11041 aatatagagc gcgtgcgcaa agaacactga aatattatta ctgagtaaag gattgttacc
    11101 gcactaagcg ggcaaaacct gaaaaaaatt gcttgattca cgtcaggccg tttttttcag
    11161 gttttttttg gagttttgcc gcaaagcggt agagggcaag ttatgacgga gttagccaga
    11221 aaaatagtcg caggagtcgg gggcgcagat aacattgtta gtctgatgca ttgcgcaacg
    11281 cgattacgtt ttaagttaaa ggatgaaagc aaagcgcaag ctgaggtact gaaaaacacc
    11341 cccggtatta ttatggtggt ggaaagcggt ggccagtttc aggtggtcat aggtaaccat
    11401 gtggccgatg tcttcctggc ggtcaacagc gtggcaggcc ttggcgaaaa agcgcaacag
    11461 gcaccggaaa atgatgagaa aggtaatctg ctaaaccgct ttgtttatgt tatttcaggt
    11521 atttttacgc ctctgatcgg tttgatggcg gcaaccggga tcctgaaagg tatgctggct
    11581 ctggcgctca cttttcagtg gacgaccgaa caaagtggta cttatttaat tttattcagc
    11641 gccagtgatg ccttgttttg gttcttcccg ataatcctgg gatacaccgc ggggaaacgc
    11701 ttcggcggta atccatttac tgccatggtg attggtggag cgttagtgca tccattaatt
    11761 ctgactgctt tcgagaacgg gcaaaaagcg gatgagctgg ggctggattt cctgggtatt
    11821 ccggtcacat tgttgaatta ctcgtcatcg gttattccca ttattttttc tgcctggttg
    11881 tgcagcattc tggaacgccg acttaatgcg tggttaccgt cggcaatcaa aaatttcttc
    11941 acaccattgc tatgtctgat ggttatcaca cccgtcacct ttctgctggt ggggccgcta
    12001 tcaacctgga taagcgaact gattgccgcc ggttatctct ggctttatca ggcggttcct
    12061 gcatttgcgg gcgcggtaat gggcggcttc tggcaaatct tcgtcatgtt cggactgcac
    12121 tggggcctgg tgccgctgtg tatcaataac ttcaccgtgc tgggctacga caccatgatc
    12181 ccgctgttaa tgcccgccat tatggcgcag gtcggggcgg cgctcggcgt cttcctctgc
    12241 gaacgtgatg cgcagaaaaa agtggtggcg ggatcagcgg cgttgacggg cctgtttggt
    12301 atcaccgaac cagcggtata tggcgttaat ctgccgcgta agtacccctt tgttatcgcc
    12361 tgtatcagtg gggctttggg ggccaccatc attggctacg cgcaaacgaa agtgtactcc
    12421 tttggtttgc caagtatttt caccttcatg caaaccatcc cgtcaacggg aattgatttc
    12481 accgtctggg ccagcgttat tggcggtgtc attgccatcg gttgcgcatt tgtcggtacg
    12541 gtgatgcttc atttcatcac cgctaaacgt cagccagcgc agggtgcccc gcaagagaaa
    12601 acaccagagg ttattacacc acctgagcag ggcggtatct gttcaccgat gacgggagag
    12661 attgtgccgc tcattcacgt cgctgatacc acgtttgcca gtggcctgtt gggtaaaggt
    12721 atcgccattc tgccctcggt tggtgaagtg cgttctccgg ttgcgggtcg aattgcttcg
    12781 ttgttcgcca cattacacgc tattggcatt gagtcagatg atggtgtgga gatcctgatt
    12841 catgtcggta tcgacaccgt aaaactggac ggcaaattct tttccgctca cgtcaacgtg
    12901 ggtgacaagg tcaatacagg cgatcggctg atttcttttg atatccctgc tattcgcgag
    12961 gccggatttg atctgacgac gccggtatta atcagtaata gcgatgattt tacagacgta
    13021 ttaccccacg gcacggcgca gataagcgct ggtgagccgc tgttatccat cattcgctaa
    13081 cgataaaagg agttaattat gaaagcattt ccagaaacat ttctttgggg tggcgcaaca
    13141 gctgccaatc aggtggaagg tgcctggctg gaagatggca aagggatcac gacctcagat
    13201 ttacagcctc atggcgtaat gggaaaaatg gaaccgcgca tcctggggaa agagaatatc
    13261 aaagatgtcg ccatcgattt ttatcaccgt tacccggaag atatcgcgtt atttgccgag
    13321 atgggcttca cctgtctgcg tatttccatt gcctgggcgc gaattttccc tcagggcgac
    13381 gaagccgaac cgaatgaagc ggggttagcg ttttacgatc ggctgtttga tgaaatggcg
    13441 caggcgggga tcaagccgct ggtaacgtta tcccattacg aaatgccata tgggctggtg
    13501 aaaaactacg gcggttgggc taatcgggcg gtcatcgatc acttcgagca ttacgcccgc
    13561 acggtcttta cccgctacca acataaagta gcgttatggc tgacgtttaa tgaaatcaat
    13621 atgtcgttac acgcgccatt cacgggcgtg gggctggcag aagagagtgg cgaggcggaa
    13681 gtttatcagg cgatccatca tcaactggtt gccagtgcgc gggcagttaa agcctgtcat
    13741 agcctgatcc ccgaagcgaa aatcggcaat atgcttctcg gcgggctggt ttaccccctc
    13801 acctgccagc cacaggatat gttggaggcc atggaagaga accggcgctg gatgttcttt
    13861 ggtgatgttc aggcgcgtgg tcagtatccc ggctatatgc agcgtttctt ccgcgaccac
    13921 aatatcacca ttgagatgac tgaaagtgac gcagaagatt taaaacatac cgtagatttc
    13981 atctctttta gttattacat gactggttgt gtttcccacg acgaaagcat taataaaaat
    14041 gcgcagggca acatactgaa tatgatcccc aatccgcatc tgaaaagttc agagtggggg
    14101 tggcaaattg atccggttgg attacgggtt ttgttaaata cgctttggga tcgttatcaa
    14161 aaaccgttat ttattgtcga gaacggatta ggcgcaaaag acagcgttga agcggatggt
    14221 tcgatacagg acgattatcg aattgcctat ttaaacgatc acctggtaca ggtaaatgaa
    14281 gcgattgccg atggtgtgga tattatgggg tacaccagtt gggggccaat tgatttagtc
    14341 agtgcatctc attcacaaat gtctaagcgc tacggcttta tttatgtgga tcgtgatgat
    14401 aatggcgaag gaagcctcac aagaacgcgt aagaaaagct tcggatggta tgcagaagtg
    14461 atcaaaacgc gggggctgtc attaaaaaaa taaccaataa agcaccttaa ttatcgtcgc
    14521 attcagaaca gtctggatgc gatacgttaa ttctttcttt gcaccataaa gggatattat
    14581 gtttagacga aatcttatta cctctgccat cttattaatg gcaccgttag ccttttctgc
    14641 acaatcattg gctgaatcat taacggtgga acatcgcctt gagttattag aaaaagcgtt
    14701 aagagaaacg caaagcgaac tcaaaaagta taaagatgaa gagaagaaaa aatatacgcc
    14761 agcgacggtg aatcgtagcg taagtacgaa tgatcaaggg tatgccgcca atccgttccc
    14821 gaccagtagt gccgcaaaac ctgatgctgt actggtcaaa aatgaagaga aaaatgccag
    14881 tgagacaggc tcgatttatt cttccatgac tctgaaagat ttcagtaagt ttgtgaaaga
    14941 tgaaattggc tttagttaca acggctatta ccgttctggt tgggggaccg cctctcatgg
    15001 ttcacctaaa tcatgggcga ttggttctct gggccgcttt ggtaacgaat actccggctg
    15061 gtttgatttg cagttaaaac aacgtgtcta caacgaaaac ggcaaacggg ttgatgccgt
    15121 tgtgatgatg gatggtaacg ttggtcagca gtactctacc ggctggtttg gcgataacgc
    15181 tggtggcgag aactttatgc agttctccga tatgtacgtt accaccaaag gtttcctgcc
    15241 ctttgcgcca gaggctgatt tctgggtggg taaacacggt gcgccgaaaa ttgaaatcca
    15301 gatgctggac tggaaaacgc agcgtactga tgccgcagcg ggtgtaggtc tggaaaactg
    15361 gaaagtcggt ccgggtaaaa ttgatatcgc gctggttcgc gaagatgtcg atgattacga
    15421 tcgcagcctg caaaacaaac agcagattaa taccaatact attgatttac gctataaaga
    15481 tatcccgtta tgggataaag cgaccttaat ggtgagtggt cgttatgtca cggcaaacga
    15541 aagcgcatcg gaaaaagata atcaggataa taacggctat tatgactgga aagatacctg
    15601 gatgtttggc acatctttaa cgcagaaatt tgataaaggt ggcttcaacg aattctcctt
    15661 cctggttgcg aataactcta tcgccagtaa ctttggccgt tatgctggcg caagtccatt
    15721 taccaccttt aatggtcgtt attatggtga tcacaccggc ggaacagcag ttcgtctgac
    15781 ttcacagggc gaagcctata tcggcgatca tttcattgtg gctaacgcga ttgtttactc
    15841 cttcggtaac gatatttata gctacgaaac aggcgcacac tctgatttcg aatctattcg
    15901 tgcggttgtt cgcccggcct atatttggga ccaatataac cagacaggtg ttgaactggg
    15961 ctatttcacc cagcaaaaca aagatgcgaa tagtaataaa tttaatgagt ctggttataa
    16021 aaccacgctc ttccatacct ttaaagtcaa taccagtatg ttgacctcgc gtccggaaat
    16081 tcgtttctac gccacgtata tcaaagccct ggaaaacgaa ctggatggct tcaccttcga
    16141 agacaataaa gacgaccagt ttgctgtcgg tgcccaggct gaaatctggt ggtaatggaa
    16201 cttaatgaaa ggaatattac gatgaatata aaaattgctg ctttaacgct ggctattgcc
    16261 agcggtattt ctgctcagtg ggccatagca gcggatatgc cagccagccc ggcacccact
    16321 atcccggtga aacagtatgt gactcaggtc aatgccgata acagcgtgac ctttcgctac
    16381 tttgcccctg gggcaaaaaa tgtctctgta gtggtgggtg ttccggttct ggacaatatt
    16441 cacccgatga ccaaagacga agcaggagtc tggtcgtggc gcacacctat cctgaaaggc
    16501 aacctgtacg agtatttttt caatgttgat ggtgtacgca gcattgatac aggcaccgca
    16561 atgactaagc ctcagcgcca ggttaactcc agtatgattc tggtgccagg cagttatctg
    16621 gatacgcgtt ctgttgcgca tggtgatttg atcgccataa cttaccactc caacgcattg
    16681 caatctgaac gtcagatgta tgtctggacc ccgccaggat acaccggcat gggcgagcct
    16741 ttgccagtgc tctatttcta tcacggtttt ggtgataccg gacgttccgc tatcgatcag
    16801 gggcgtatcc cgcaaatcat ggataacctg ctggctgaag ggaaaattaa accgatgctg
    16861 gtggtgatcc cggataccga aaccgatgcg aagggcatta ttcccgaaga tttcgtgcct
    16921 caggaaagac gtaaagtctt ttatccgctg aatgctaaag cggcagatcg cgaactgatg
    16981 aacgatatta tcccgctgat tagcaagcgt tttaacgtca gtaaagatgc cgatggtcgc
    17041 gcactggcag ggctttcaca aggtgggtat caggcgctgg tatcgggaat gaatcatctg
    17101 gaaagctttg gctggctggc cacattcagt ggtgttacca cgacaaccgt accggatgaa
    17161 ggtgtcgcgg cccggctgaa cgatccggca gctattaacc agcaactacg taattttact
    17221 gtggtcgttg gtgataaaga tgtcgtaacc ggcaaggata tcgccgggct gaaaactgag
    17281 cttgagcaga aaaaaattaa gtttgattac caggaatatc ccggcctgaa ccatgaaatg
    17341 gatgtctggc ggcctgccta tgcagccttc gtacagaaat tatttaaata aaataaggca
    17401 ctaagatgaa attaatcatt accgaagatt accaggaaat gagccgtgtc gcggcacacc
    17461 atctgcttgg ttatatgtcg aagacgcgtc gtgttaacct ggcaattacc gccggtagca
    17521 cgcccaaagg catgtatgaa tacctcacca ccctggttaa aggtaagccc tggtacgata
    17581 actgctattt ctataatttc gatgaaattc cctttcgcgg caaagaggga gaaggtgtaa
    17641 cgattaccaa tctgcgtaat ctgtttttca cccctgcggg gatcaaagaa gagaatatcc
    17701 agaagctcac tattgataac taccgcgagc atgatcagaa actggcgcgt gaaggcggcc
    17761 tggatttggt ggtgctggga ttaggtgcag atggtcattt ttgtggcaat ttgccgaata
    17821 cgacccattt ccatgagcaa accgtggagt tcccgatcca gggagagatg gttgatattg
    17881 tggcgcatgg tgagttgggc ggcgactttt cgttagtgcc tgacagctac gtgacgatgg
    17941 ggccgaaaag catcatggcg gcgaaaaact tgctgataat tgtcagtggc gcaggtaaag
    18001 cgcaggcact gaaaaatgtt ctgcaaggtc cggttaccga agatgttcca gcttccgtgt
    18061 tgcagctgca tccttcgctg atggtaatcg ctgataaagc cgctgccgca gaattagcgc
    18121 tcggttaagc tgaagaaata aggccggatg tatgaattac gctccggcct gttctcatta
    18181 tttaaataac tacgaaaaat caccccacag tctcagcttg ccgcagtgga gacagcggaa
    18241 gagataaccc tgacaatcac cacccttttg caggcattta gctagatcag aatttctaat
    18301 accgaaattc tcacaatctt cttcaaggtt ggcaaattca tcgaggcgat cttttatatc
    18361 attccagccc acatagccga taaaagcaca gaaatcgcca caatgcgcaa gccagaattc
    18421 ctgttgccat ccatgataac ctggcgtgcg ttcaaccaac tctttcagca tttcatcagg
    18481 atatgtgttc ttaataccgg caaattcgtc ctcttcatca tactcaaatt caacaccttc
    18541 tatgctggca tcatcctgaa aactacctgc aaacttttca gcagcagaac cgtccgcaat
    18601 acaccacgga cagagatgtt caacttcatc aacgcaataa aagggacccg agtaataaac
    18661 tgacgtctgt tgttcacagc aatcgcactc tacggtttta tcctgttcaa atgcgcctgt
    18721 ttccagtggc ttgggatgat atttgaattg gggtaacggc ctgatatttt gagtcatagt
    18781 aacatcctgt ctgcttttga gtagcccaaa gataaagtat cccgcttaac ttaaagagtc
    18841 tgtttcagga catgaaatat ccaatccggg cagacgggta atgattctgc tcatcaccac
    18901 acctgccagc aatgcaggaa tgatgcggac aacgagactg tcaccagaaa gaatgttttc
    18961 aaacaactcc gaggaagcca tgcccttaat atgtacaata actgcacaat agatctcggt
    19021 gataacgacg ccacctatgc caccagccag acaacgataa tttttctgtg acccgatctt
    19081 ttccggcaga caggcgacca tcacgccggt taacaatgca ggaataactc ccgtagtaat
    19141 cgacaataag agaaacaatg gaagtacctg tattcttcgc aaaagaacca gcggttcttt
    19201 tgcgaagaat atgtagagga caagaaacag aacaccaatt aacggtccta aaaccgcaaa
    19261 ataaagtccg tgatgtatta cccgacgtga aacagacata tcaaactccc tttacttacc
    19321 tttgagcgcc aatttaagcg gggtaagtga agggagtgtg aagaactatg ccgtaatatc
    19381 ccaaccacgc gctttccaca gttccggtaa ctgcgaaaga tgggtaaagg tggtgacttt
    19441 cgggtgaacg atcggcttat tgtgcgggtc ggcgcagaag tagaacactt ccatacctgc
    19501 gtcgatacca gattgcgcac cggcaactga gtcatcaacc agaatgcagt tttctacatt
    19561 tacattcatc gcttttgccg catggaacat taacgccggg tctggcttcc agcgctgaat
    19621 atcgtagccg ctgaacagtt tatccgggaa gtagtgcaac atattcagct tgcccataga
    19681 atgctgcatt ttgttatttg ggccgttaga taccacacac attggcgcag tgatcgctga
    19741 caggagcgct ccagcccctt cgatggcttc cagttctgaa tcgaacagcc gagcgacttc
    19801 tgcacggtaa acgtgttcag cttctgtttt cgctaacgta acaccatgtt caagggaaac
    19861 aatatcgata atttcgtaca gttttacacc tttgaaacgt ttgaatacct cttcaggatc
    19921 gagcgtaata ccaaattcct gaaacatcgt tacatatgcg cgggagcaaa tgacttcact
    19981 gtcgaccagc gtaccgtcgc agtcgaaaaa taccgcttct atctgggaca tgtctttcct
    20041 ttatcgttag tttaacgttt tcgtcatgcc aattcgcatg acgcaatcgt tgccgtataa
    20101 gcaaatacaa tgaaaaaaag ttatcttcag ccgtccatat tgtctcattt tggtatacga
    20161 tagcgacgga ttttccctcc ttgttcggaa atagataatg agtcaacaac acacaaccca
    20221 ggcttctggc caggggatgc tggaacgcgt gtttaaactg cgcgaacatg gcacgacggc
    20281 acggaccgaa gtgatcgccg gttttaccac cttcctgacg atggtttaca tcgtttttgt
    20341 taacccgcaa attcttggcg ttgctggcat ggataccagc gccgtcttcg tcactacctg
    20401 tctgatcgct gcattcggca gtattatgat gggactgttt gctaacctgc cagttgcact
    20461 ggcacccgct atgggcctga atgcgttctt cgcttttgtc gttgtacagg cgatgggctt
    20521 accgtggcaa gtcgggatgg gcgcaatctt ctggggtgcg attggtctgc tgttactgac
    20581 gattttccgc gttcgctact ggatgattgc caacattccg gtaagtctgc gtgtgggcat
    20641 caccagcggt atcggtctgt tcattggtat gatgggactg aagaatgcag gtgtgattgt
    20701 cgctaacccg gaaacgctgg tcagcatcgg taacctgact tctcacagcg tgctgctggg
    20761 gatcctcggc ttcttcatca ttgctattct ggcctcgcgc aacattcacg cagcggtgct
    20821 ggtttctatc gtggtgacga cgctgctggg ctggatgctg ggtgatgtgc actacaatgg
    20881 catcgtttct gcgccgccga gcgtaatgac agttgtgggt catgtagatt tagccgggtc
    20941 gtttaacctc gggctggcag gggtgatttt ctctttcatg ctggtcaacc tgtttgactc
    21001 ctccggtacg ctgattggcg tgaccgataa agcaggcctg gcggatgaga agggtaaatt
    21061 cccgcgcatg aagcaggcgc tgtatgtcga cagtatctct tccgtgaccg gttcgtttat
    21121 cggtacttct tccgttacgg cttatattga gtcctcttcc ggcgtatcgg ttggcggtcg
    21181 taccggtctg acggcagtgg ttgttggtct gctgttcctg ctggttatct ttctgtcgcc
    21241 gctggcgggg atggtgccag gctacgctgc agctggtgcg ctgatttacg ttggcgtgct
    21301 gatgacttcc agtctggcac gcgtgaactg gcaggatctt actgaatctg ttccggcgtt
    21361 tattaccgcc gtgatgatgc cgttcagctt ctcgattacc gaaggtatcg cgctgggctt
    21421 tatctcctac tgcgtgatga aaatcggtac cggacgtctg cgtgatctaa gcccgtgcgt
    21481 aatcatcgtt gcgctgctgt ttatcctgaa gattgtcttt atcgacgctc actaaaatcg
    21541 atagcgcgtt aaaaacccgc catgcatgat tgctggcggg ttttttattt agatcttaac
    21601 tcgctgaata aactcaccaa atgcggtcaa ttgcccggtc aggtggtcca gcgtaccctg
    21661 atcaatcact tctccggttt gcggatcaac tttgttctga atcacgccgc ccataaattc
    21721 cggcttgttc atcaccattg catcgaggaa aaccagaatc tggcgcaggt gatactgaca
    21781 gcgcgcgccg ccaatcacgc ccattgagct ggtctgaatc aacaccggtt taccggccag
    21841 cggttgatcc ggcaggcggg aaagccagtc gatggcattt ttcagcccac ccggtaccga
    21901 gtagttatat tccggcgtga cgatcaccac accgtcagcc tgacggatct gttccgccag
    21961 agcttcaacc gttgctggaa aaccttcttc ctgctgtacg tcagcgtcat acaagggaat
    22021 gtcggcaatg gaaggtaacg cattgacttc catgctcgcc ggagcaattt tcggcagggt
    22081 acgtgcaacc atgccattaa atgagccttt gcgcaggctc cccagtaacg taaccacctg
    22141 caatttttca gacatgatga ctcctgtggt tgtctatcaa tttggcgaaa aagccttcac
    22201 ggtagagagc tgggcaaaac gcatcatccg gctggtagga ttgttggcgc ggctgtgaat
    22261 atccggcagg cttttccagc cgtgtttgcc atcgaactcc cagacactga gcttttcaat
    22321 cgtgggcgtg accgccacgg accacaccag cacgtcctcc gcgtcgcata acgcttctac
    22381 atgatttaca tgagcacatt tcaggcgtcc ggcaatgggc agcagctgca gatcgcgcgg
    22441 atcgtcattc aaaacatcac cggttagttt tagcacgctt cggcgcagcg tgatgagctg
    22501 cgcccgcgct tcgttaggat cgttttgttt actgatccat agcgattcat tgctggaaaa
    22561 ggtgtggtta tcgggcgcgt gtgggctatg aaacccgcgc gtcgcacgct gtagttccat
    22621 atcgagacca cattcacctt cggttgttaa cgccacgcca accatattcc cggcatagga
    22681 aatagaaaac gaaggcaaat ttttatcact gaaaaccggt ttacctttcg gcagggtgac
    22741 gatttccggc aattcgccaa tgccatacag catgaacatc agttctgcga gtaaacctct
    22801 agacgccaga aaacggcttt gacgatgagg gggaatgttt cgggcttctt gatgacactg
    22861 ggaggggaga cgaacagaaa tcagatgtcc ttccgttaaa atccctcggg caaagtgagt
    22921 cgccattttt cgctccatgc ttaatggtcc attatgaata caagagtaat catttactgg
    22981 aatgatggac agactaaagc ggctaaaagt tcgattcaga caagcttatt acaacttttt
    23041 tacattttat tgtgaaattg ggtcgcgttt catacgattc ggttatgcca tcgacgcgta
    23101 aaggttttta atggtttccc gtaaccagac gatcttcgga ttatggctgt tccgtttatg
    23161 ccacagcagg gtaaaaggaa cttccagctt tttttgctgg ctttcgtcaa acgggagagg
    23221 aagagcaacc aacggcagtt gatggagttg attgtagtac tgacagtagc gcggcgcggt
    23281 cgccagtagc agattgtcgg gttgcgctgc cataaacagt gactgctcaa attccggcag
    23341 gctcatagca atcgtgcgtt cgcgccccag ctcctgtaac acattgtcca gcgcccaggt
    23401 gtcgctctgt tcccagcaaa tgctgatatg cggataacgt aaaaaggtgt ccagattcca
    23461 cgtttcatgc agtgccggat gatctttgcg taaccagacg cagggtacat cactaaacag
    23521 cacttcataa tcaatggcta acggtagcga gcttaacagc tcccgcgagc gaggatggct
    23581 ttcgcgaccg gaaaaaccaa tatccacttc accacgagta atggcatcta aggaatcgta
    23641 atcccagtta cgtagtttga tggtcgcctg cgggtaacgt tggtagatcc gtttcgacag
    23701 cgcattaagc atgatcatca tcagcggcga ttccgccgcc aactcaaact tcaggccgcg
    23761 cggtgtctgg tggtgcggtt tatccagcag cagattgctc atttgcatcc attccgccag
    23821 attttgctcc atgctgacca ttagcggtgt gggcgacagg cccagcggtg agttcacaaa
    23881 gagcgggtcg tcaaaccacg ctcttaactt tgccagcgac ttactcaccg ctgaaggggt
    23941 cacgttcatc cgcttcgccg ctttggtcac gctgcgctcc tgcatcagca gttgcagaca
    24001 gagcagcaga ttgagatcaa gcgtggtgat ggatttcttc atgagcggca acggggcgtc
    24061 caggcgcgac gaacataatc agcaacaggc tcactatgct acaggcaatc agaatcccga
    24121 tcagcatatt ccatgcgccg ataccaacca ccgctgccag ccaaatccac agtgacgaac
    24181 cgcaaacctg cgcaatacct aaggtcgagc tggctacgcc cgcgcgtaat gaaaatggtc
    24241 ctaacgcctg actcatcgct acgccaaaac ctaccgagaa accggcgcaa atcagcgtga
    24301 taccaaacag agaaaccgca tgggaaggtg aaacggcaag ggtgatcccc gctgcaagga
    24361 ataacacctg cgaggtgatc atcaacgtac gtggcttaaa aattcccagc gcaaatggcg
    24421 tggagaatga aacggtcatg ctgacgccag cagtcaacgc cataatggtg gcgtattctc
    24481 cgcgctcaaa acccatgatt tccatcagca ataccggcga tgtattgacg aaagtgagga
    24541 tcaccgaaac gctgagggtg gtgataacaa cacggctgag gaaaaaccga ttaagcagcg
    24601 actcgctatt ttctcgtggt ttgtccgaag cagcggggga cgctgggcgc gtttctttta
    24661 aaataaacaa agacaacatc agtaccgcga tgcccatcat tgccatcgcc cagaacagac
    24721 tctgccacgg gaatttaagc ataatcagat gtccgagcac tggcgctaac accggaatga
    24781 tgcaggtaat accgttgagt aatgacagca ctttagcccg acgtcgatca tccagcgtgt
    24841 cgcgcaaaat ggcgaacgcc actacgtaac aacagcctgc gcccaacccc tgtagaaatc
    24901 ggcctgcaag aaataacgtg ctggtttcag ccagtgaaca gaacaccgaa gcgataataa
    24961 atagcgccgc gccggggatg gcgacaggct ttctccctga acgatcggcc actttaccgg
    25021 caaataacat cgcagctgcc atgcccgcca gataaacgga gaacgcaata tgcaactgcg
    25081 cttcgctggc attgagatcg tcggcgatgc gcggtaaacc aacgaggtac atatcaatcc
    25141 cggcgggata aagtaaaacc agggcaaaac tacaaattaa aaagcgggac atgggctaaa
    25201 gcgtcctaat agtaaggtga cgcaaagcat acgtggtgaa ggagaatcag gcgagttgcc
    25261 atttggacat aagtgatttc ctgagaggca agaagccagc gaatggctgg cttcttgaag
    25321 gatttagcca aatttaggta acacgttaaa gacgttgccg aaccagcaca aaatcacggt
    25381 tataccaaag agaatgacaa tcgccggaat aagattgccg ccccagaccg tgaacatctg
    25441 attgggaaac ttcttgcgag ctttgattgc aagcactgcg ggaataatga ccgcccagat
    25501 ggtggcgcac agcccggcac cgccgatccc gtaaataaag ccgttcggga agatcagata
    25561 caacaacgca ggtggcagga aggttaacag cacggttttg aaacgcccgc cgtgggagtt
    25621 atcaatctta aacaggtccg ccagataatc gaacaacccc agcgtgacac caaagaacga
    25681 actggcaaca gctaagttag agaacaccag caggcaaaac tcgataatgc cgtgctgttt
    25741 ggtgccgagg aacgatttca ccagcgaatc aacgttgccg cctgaggaga ttatcgcctt
    25801 aaagctttcg cgcggaatat tccccatcgt gcaatagagc cagaagagat aaatcaccag
    25861 cgccagcagc gaaccaaata ccacgctttt gattaactta tctttgcgtt ttccatagca
    25921 aataatcagg ctgggaatat tgccgtggaa accaaatgac gccagacaca ccggcaaagc
    25981 cataaagata tacgggaagt aagacgttcc cgcagtggtg ctggtggcat cgcgcaggat
    26041 ggagtaatcg acctggaaga agaaagaacc aaacacgatc acaaaggaga taatcttcag
    26101 cccgaggaac aatgaggtaa tgcgactggc ggctaacgaa cttatccaca atacgctggc
    26161 aacgaaaatg gctgtgcaga tcccgacaat acgtggatta gcgtgatagc ccaaattcat
    26221 tgatatcgtt tcactaatga tcgcaccatt agcagagata taggcataag tgaggatata
    26281 gagaacgaag gcaacggtaa taccgctgat aatgttccag gtgttaccga ttaaatcttt
    26341 ggtgatggtg ttaaaactgg agccgacagg ataatttaaa tttgcttcta ataacaataa
    26401 cccggaatga agcattgaaa accaggcaat gataaggata aaggcacccc agaaaaacca
    26461 ggcaccggca agatcaacag gtaaagcaaa catacctcca ccaattactg tacctgctat
    26521 aaccataaca ccccaaaatg cagagtgctt tttttcagct tgatcagtca tgatgccacc
    26581 tttagaggaa ggctattttt gttattgagg atgtagggta agagagtggc taacatcctt
    26641 atagccactc tgtagtatta attaaacttc tttcagtttt gcggtgaagt gacgcaatac
    26701 ttttggttcg taggtaaagg ttaatccttt aatattcgcc gcgttctctt tcacatgttt
    26761 aaaggcttca ataatgaagt ccatatgtgt ttgagtatat gttgcgcgcg gaatggttaa
    26821 acgcagcagt tcagccgggc atggcagttg tttaccggtt ttcggatcgc ggcctaacag
    26881 gaaagagcca atttctaccg cacggatacc ggcgacttta tacagctcgc acgccagcgc
    26941 ctgtgccggg aactggtctg ccgggatatg cggcagcagt ttaccggcat caacgaatgc
    27001 cgcgtgaccg cccgcctgct ggcagacaac gccaatctct tccagaccat cgaccagata
    27061 ctgtacctgc gcgatacgat aagccagcca gtcgagattc atgccgtcat acagacctac
    27121 cgccagacgc tccatcgcgc cgccttccag gccgccatat gtcgggaacc cttcctgcac
    27181 cacgcaaagg gttctgcact cggtgtacac atcaaagaag ctgtcgtctt tcatgcacag
    27241 caagccgccc atcggtacca tcgcatcttt cttggcggac atcgccagca tatcggcata
    27301 tttgtaggtt tcgcgggtga tctgctcgat ggtccagtct ttgtattctg cttcacgctg
    27361 cttgatgaaa taggcgtttt cagcaaagcg tgcggagtcc attaccaccg gaatatcgta
    27421 tttcttcgcg atgctgtaca tcgcttttaa gtttgccagt gaaaccggct gaccacctgc
    27481 agagttactg gtgatggttg caacgatata cggcacgtta ttcgggccaa cttcttcaat
    27541 accgcgttct aatccctcaa ggtcaaagtt acctttaaag tcgtaacgca cgccagtatc
    27601 gaaggcttct ttgatataga cgttacgcac ggtacagccg ttaatctggc tatggccctg
    27661 cgtggtatca aagaaatagt tagagaatgc caccattttg ctgcgatcca ggcctttttc
    27721 ctgctcgcgt tttttaatca gtaccggaat ataaatttgc tctgcgccac ggccctggtg
    27781 agtcggaatg gtgtattgat aaccaaagat atttttcact gactcggcta acgcatagta
    27841 gctgcggctg ccgctgtagg cttcgtcgcc gcgcatcatc gctgcctgca tactttgggt
    27901 taccgccccg gtgccgctgt cggtcagtaa atcgataaac acatcttcgc tatccagcag
    27961 gaacgggttc ataccggatt taataattgc ttcttcacga taagcgcgag tagtacgttt
    28021 tactggctca ataacacgaa tgcggaacgg ttcagggaga tgtttaaagt tttccattac
    28081 ataatccttc atttatttta attacagtga tccctgtgaa tattacatct gcaatatcca
    28141 ttaattcgcc atgtatatat acaagcacca tcatttacta aaataagtaa ataaacgaga
    28201 gatggccttt gcaaaaggca aaattaagat atagcaaact aatcgttcac attgaatcta
    28261 atcggtttgg ctctggtgat ggctacagaa gggtaaatta agggcggtga tcgacaatct
    28321 tattgtcgat attgaaccat tttgaggtca cacatatatg taagatattc ataatgcact
    28381 tatcctcgaa agacacgagg ctacagtata atactcaaga attttgtcta ttctaaaatt
    28441 gtttaaatgt gaatcgaatc acaatcgttc ggggagcaat attaaatgct tttttttaag
    28501 attataaatt acgtaagaat tttaatgttt aaaaaaaggg ttgaggaata acaggagtgc
    28561 taacaatgaa gcgaagccgc atctgacagt cagaatacgg cttcgtaagc gcggttactt
    28621 accaatacag aagctggaga aaatccgccc cagcaggtcg tctgaagtaa attccccggt
    28681 gatttcgctt aagttctgct gtgccagacg caactcttcc gccagcagtt cacctgccca
    28741 ggctcccaac agttgcgctt tgccctgttg tagatgttcc gctgcctgtt ccagcgcctg
    28801 taggtggcga cgacgcgcca ggaagccgcc ttccatgttg gtgtcaaagc ccatgctctg
    28861 tttgagatgg ttacgcagca cgtccacgcc ttcaccagtc cttgccgaga gacgaattaa
    28921 cgcgtgaccg ttcacttcac tcattcccag cgtttcgccg gtgatatcgg ctttattgcg
    28981 caccacggtg atcggcagtt tcgctggcag acgggcaata aattccggcc agatctctgc
    29041 cggatccacg gcgtctgttg tggtgccatc gaccataaac agcacgcggt cggcctgttc
    29101 aatttcctgc cacgcgcgct cgataccaat acgttccact tcgtcactgg cttcacgtag
    29161 cccagcggta tcgatgatat gcagcggcat tccgtcaatg tggatatgct cacgcagcac
    29221 gtcacgtgtg gttccggcga tatcagttac gattgctgct tcacggcccg ccagcgcgtt
    29281 taacaggctc gatttaccgg cgttaggacg tccggcaatc accaccttca tcccttcgcg
    29341 caacaaacta ccctgacgtg cttcagcacg cactgcatcg agatcggcaa tcacgtcatt
    29401 aagctgggct tcaattttgc cgtcggagag gaaatcgatc tcttcatcag ggaaatcaat
    29461 tgccgcttcg acgtaaatgc gcaagtgggt gagggcttcc accagatggt tgacccgtgc
    29521 ggagaatgcg ccttgcagcg agttaagtgc cgaacgggcc gcctgttccg aactggcgtc
    29581 gataagatcg gcaatcgcct cggcctgggc taagtcgagt ttatcgttaa gaaacgcgcg
    29641 ttcggaaaac tcaccagggc gagcaatccg caggccggga atggtcagaa tgcgttttaa
    29701 cagcaggtcg aggatcaccg gaccgccatg accttgcagt tccagcacat cttcgccggt
    29761 gaacgagttc gggccaggga accatagcgc aatcccctga tcgagcacgc tgccgtcggc
    29821 gtctttaaac ggaagataat cggcgtagcg cggcttaggt agtttaccca gcacggtttc
    29881 ggcaacttca cgggctttga agccggagat gcgcaggatg ccaacgccgc cacgtcccgg
    29941 aggcgtggcc tgggctacga tagtgtcatt atcgctcatg atgttcctgt tgctttgtgt
    30001 ggcggatgcg cggtgcttat ccgccctacg aaaagaaaaa aggcggtcaa ctgaccgccc
    30061 ttattttagc gaaaactcac cgaatcagga ttttttcttc tcgcggctat gcaggccacg
    30121 tttttccaga ccacggtaaa tcagctgctg ctgaataatg gttaccaggt tgctgacgat
    30181 atagtacagc accagacctg acgggaacca caggaagaac acggtgaaga tgaccggcat
    30241 aaaggtcatg atcttctgct gcatcgggtc ggtcactgtg gtcggcgaca tcttctgaat
    30301 gaagaacatc gttacgccca tcaggatcgg caagatgtag tacgggtcct gtgccgacag
    30361 gtcgtggatc cacagtgcaa acggtgcctg acgcagttca acggaaccca tcagcatgta
    30421 gtacaacgcc aggaagattg gcatctggat cagcagcggg aagcagccgc ccagcgggtt
    30481 aaccttctca gctttgtaca gcgccatcat ttcctggctg atacgctgtt tgtcatcgcc
    30541 cagacgctca cgcattgcct gaatcttcgg ctgcagcata cgcatcttcg ccatggaggt
    30601 gtactgcgct ttggtcagcg ggtacatgat gccacgaacg ataaaggtga tgatgataat
    30661 ggagaagccc cagttaccca caaagctatg gatccatttc agcagtttga acagcggctg
    30721 agagatgaac cacaaccaac cgtaatcaac ggtcagatcc aggtgcggag caacagctgc
    30781 cattttgtcc tggatttccg ggccaaccca cagggtgctg ttcatcgcgc cagtctgacc
    30841 aggctgaacc agtaccggct gagatttata gccgatagcg gcgatgccgt tacccagatt
    30901 agcggtatag aagttgttgg taccgtcgtt atgcgggatc cacgccgtcg cgaaatactg
    30961 ttgcagcatc gccacccaac cacctttcga agagatgttc aggttttcgt tatcggcaat
    31021 ggtatcgaac ttgtatttct cgtacttctc gtcaggcgtg gagtacgccg cgccgcggaa
    31081 ggtgtgcagt gcgaagttgc tgcttccggt atcgagatgc ggtggcagag tgatggattg
    31141 cttcaactga ccaaaggtgg agatttccag cggtttctcg ccagcgttct gcacgttgta
    31201 gttgacgttg acagcgtaat caccacgttt caggacaaac gttttggtaa acgtgttgcc
    31261 tgccgcgtcg gtatacgtca tcggcacctg cagttcgttt tgaccttcag ccagcacata
    31321 agcgtctttt tcaacgttat acagcggacg cgggccgtta gccgggttat ccgggccatc
    31381 acgaccggtc agaccgctct gtgcctgata aataaactgc ggtgaagttt ccagcagctg
    31441 gaacggctgg gtagagttca gctctttcgg gtaagcaggc agcagagctt gctcaacatc
    31501 accaccacgg gtgttgatgg tcagatcaag cacgtcggtc ttaaccgaga tcagtttccc
    31561 ctggccactg gccggtacgc cctggtcggc ggcgctaccc gctgcggtgg tcgttgtctg
    31621 cgtggtctgt tgggcctgag gttgcgggtt tttatcctgc tcccaggctt gccagatcat
    31681 gaaagacacg aacagcaaag cgatgactaa aagattgcgt tgcgaatcca tcgttagtgt
    31741 tctctggtat caaatggtcc gggcgggacg ggatcgtcac caccagggtg taaagggtgg
    31801 cattttaata cgcgtttcac cgtcaaccaa ctgcctttta tcactccaaa cctgcgcaat
    31861 gcctcaattc cgtagcttga acaggttgga gtgaaacgac aatgcggccc gagtagcgga
    31921 ctaatcaggc gttgatagac ccgaatgagg gctatcagga cccgcgagcc aggcgacagt
    31981 ggcggcgcca taatttttcc aacgcttccg agagagcacg gttatcgagg tcggcaaccc
    32041 cttttttcgc caccaccacg aaatccatag ccgggagttc atgttggcgc agacggaagc
    32101 tttcacgcgt cagacgttta atccgattgc gttcatgggc gcgtcgaacg tttttcttgg
    32161 cgactgtaag accgatacgg ggatgcccca gcgaattcag gcggccgaga atggtaattt
    32221 gcggcgtgcc agcccgttgt ggctgctgga agacgaatgt gaattgactg ggagttaaca
    32281 agcgtaactc cctgggaaat gcgagcttaa ccactcaggg gttagcttta ttacttagaa
    32341 acggtcagac gagcgcggcc tttagcacga cgacgtgcca gaacctgacg accattttta
    32401 gtagccatac gagcacggaa gccgtgagaa cggttgcgct tcagtacaga cggttgaaaa
    32461 gtgcgtttca tggcgatttc tacctaaact tgaataaatt caatggcttt attggatatc
    32521 cgccgaaaaa tgaaacgatg gacaccgaag ccatgggtga ttaaagaggc cggattgtaa
    32581 taattgtaca ctccggagtc aattctcttt ccttatttac cgcgcttttc cgcacctttt
    32641 cgcagggaaa atgtacgacc tcacaccagt ggaaaccagc atggcgcgcc gggtggagga
    32701 ttatacgggc tgatgggtaa agcgcaagga tcgtcctgga tctttattag atcgattaag
    32761 ccaatttttg tctatggtca ttaaattttc caatatgcgg cgtaaatcgt gcccgcctcg
    32821 cggcaggatc gtttacactt agcgagttct ggaaagtcct gtggataaat cgggaaaatc
    32881 tgtgagaaac agaagatctc ttgcgcagtt taggctatga tccgcggtcc cgatcgtttt
    32941 gcaggatctt gatcgggcat ataaccgcag acagcggttc gtgcgtcacc ctcaagcagg
    33001 gtcttttcga cgtacgtcaa caatcatgaa tgtttcagcc ttagtcatta tcgacttttg
    33061 ttcgagtgga gtccgccgtg tcactttcgc tttggcagca gtgtcttgcc cgattgcagg
    33121 atgagttacc agccacagaa ttcagtatgt ggatacgccc attgcaggcg gaactgagcg
    33181 ataacacgct ggccctgtac gcgccaaacc gttttgtcct cgattgggta cgggacaagt
    33241 accttaataa tatcaatgga ctgctaacca gtttctgcgg agcggatgcc ccacagctgc
    33301 gttttgaagt cggcaccaaa ccggtgacgc aaacgccaca agcggcagtg acgagcaacg
    33361 tcgcggcccc tgcacaggtg gcgcaaacgc agccgcaacg tgctgcgcct tctacgcgct
    33421 caggttggga taacgtcccg gccccggcag aaccgaccta tcgttctaac gtaaacgtca
    33481 aacacacgtt tgataacttc gttgaaggta aatctaacca actggcgcgc gcggcggctc
    33541 gccaggtggc ggataaccct ggcggtgcct ataacccgtt gttcctttat ggcggcacgg
    33601 gtctgggtaa aactcacctg ctgcatgcgg tgggtaacgg cattatggcg cgcaagccga
    33661 atgccaaagt ggtttatatg cactccgagc gctttgttca ggacatggtt aaagccctgc
    33721 aaaacaacgc gatcgaagag tttaaacgct actaccgttc cgtagatgca ctgctgatcg
    33781 acgatattca gttttttgct aataaagaac gatctcagga agagtttttc cataccttca
    33841 acgccctgct ggaaggtaat caacagatca ttctcacctc ggatcgctat ccgaaagaga
    33901 tcaacggcgt tgaggatcgt ttgaaatccc gcttcggttg gggactgact gtggcgatcg
    33961 aaccgccaga gctggaaacc cgtgtggcga tcctgatgaa aaaggccgac gaaaacgaca
    34021 ttcgtttgcc gggcgaagtg gcgttcttta tcgccaagcg tctacgatct aacgtacgtg
    34081 agctggaagg ggcgctgaac cgcgtcattg ccaatgccaa ctttaccgga cgggcgatca
    34141 ccatcgactt cgtgcgtgag gcgctgcgcg acttgctggc attgcaggaa aaactggtca
    34201 ccatcgacaa tattcagaag acggtggcgg agtactacaa gatcaaagtc gcggatctcc
    34261 tttccaagcg tcgatcccgc tcggtggcgc gtccgcgcca gatggcgatg gcgctggcga
    34321 aagagctgac taaccacagt ctgccggaga ttggcgatgc gtttggtggt cgtgaccaca
    34381 cgacggtgct tcatgcctgc cgtaagatcg agcagttgcg tgaagagagc cacgatatca
    34441 aagaagattt ttcaaattta atcagaacat tgtcatcgta aacctatgaa atttaccgta
    34501 gaacgtgagc atttattaaa accgctacaa caggtgagcg gtccgttagg tggtcgtcct
    34561 acgctaccga ttctcggtaa tctgctgtta caggttgctg acggtacgtt gtcgctgacc
    34621 ggtactgatc tcgagatgga aatggtggca cgtgttgcgc tggttcagcc acacgagcca
    34681 ggagcgacga ccgttccggc gcgcaaattc tttgatatct gccgtggtct gcctgaaggc
    34741 gcggaaattg ccgtgcagct ggaaggtgaa cggatgctgg tacgctccgg gcgtagccgt
    34801 ttttcgctgt ctaccctgcc agcggcggat ttcccgaacc tcgatgactg gcagagtgaa
    34861 gtcgaattta ccctgccgca ggcaacgatg aagcgtctga ttgaagcgac ccagttttcg
    34921 atggcgcatc aggacgttcg ctattactta aatggtatgc tgtttgaaac cgaaggtgaa
    34981 gaactgcgca ccgtggcaac cgacggccac cgtctggcgg tctgttcaat gccaattggt
    35041 caatctttgc caagccattc ggtgatcgta ccgcgtaaag gcgtgattga actgatgcgt
    35101 atgctcgacg gcggcgacaa tccgctgcgc gtgcagattg gcagcaacaa tattcgcgcc
    35161 cacgttggcg actttatctt cacctccaaa ctggtggatg gtcgcttccc ggattaccgc
    35221 cgcgttctgc cgaagaatcc ggacaaacat ctggaagctg gctgcgatct gctcaagcag
    35281 gcgtttgccc gtgcggcaat tctctctaac gagaaattcc gcggcgtgcg cctgtatgtc
    35341 agcgaaaacc agctgaaaat caccgccaac aacccggaac aggaagaagc ggaagagatc
    35401 ctcgacgtta cctatagcgg tgcggagatg gaaatcggct tcaacgtcag ctatgtgctg
    35461 gatgttctga acgcgctgaa atgcgaaaac gtccgcatga tgctgaccga ttcggtttcc
    35521 agcgtgcaga ttgaagatgc cgcatcacag tcggctgcct atgttgtcat gccaatgaga
    35581 ctgtaatgtc cctcacccgc ttgttgatcc gcgatttccg caacattgaa accgcggatc
    35641 tcgctttatc tcccggcttt aactttctgg taggtgccaa cggcagtggc aaaaccagcg
    35701 tgctggaagc catctatacg ctcggccatg gtcgggcgtt tcgcagtttg cagattggtc
    35761 gcgtcattcg ccatgagcag gaggcgtttg ttctccacgg gcgattacag ggcgaagagc
    35821 gcgagacagc gattggctta accaaagaca aacagggcga cagcaaagtc cgcatcgacg
    35881 gtacagacgg gcataaggtc gcggaactgg cgcacctgat gccaatgcag ttgataacgc
    35941 cagaagggtt tactttactc aacggcggcc ccaaatacag aagagcattc ctcgactggg
    36001 gatgctttca caacgaaccc ggatttttca ccgcctggag caatctcaag cgattgctca
    36061 agcagcgcaa tgcggcgctg cgccaggtga cacgttacga acagctacgc ccgtgggata
    36121 aagaactgat cccgctggcg gagcaaatca gcacctggcg cgcggagtat agcgccggta
    36181 tcgcggctga tatggccgat acttgtaagc aatttctccc tgagttttct ctgactttct
    36241 ctttccagcg cggctgggag aaagagacag aatatgctga ggtgctggaa cgtaattttg
    36301 aacgcgatcg ccagctaacc tacaccgcgc atggcccgca taaagcggac ttacgcattc
    36361 gcgccgacgg tgcgccggtg gaagatacct tatcgcgtgg gcagcttaag ctgttgatgt
    36421 gcgccttacg tctggcgcaa ggagagttcc tcacccgtga aagcgggcgg cggtgtctct
    36481 acctgataga tgattttgcc tctgagcttg atgatgagcg tcgcgggctg cttgccagcc
    36541 gcttaaaagc gacgcaatca caggtctttg tcagcgcgat cagtgctgaa cacgttatag
    36601 acatgtcgga cgaaaattcg aagatgttta ccgtggaaaa gggtaaaata acggattaac
    36661 ccaagtataa atgagcgaga aacgttgatg tcgaattctt atgactcctc cagtatcaaa
    36721 gtcctgaaag ggctggatgc ggtgcgtaag cgcccgggta tgtatatcgg cgacacggat
    36781 gacggcaccg gtctgcacca catggtattc gaggtggtag ataacgctat cgacgaagcg
    36841 ctcgcgggtc actgtaaaga aattatcgtc accattcacg ccgataactc tgtctctgta
    36901 caggatgacg ggcgcggcat tccgaccggt attcacccgg aagagggcgt atcggcggcg
    36961 gaagtgatca tgaccgttct gcacgcaggc ggtaaatttg acgataactc ctataaagtg
    37021 tccggcggtc tgcacggcgt tggtgtttcg gtagtaaacg ccctgtcgca aaaactggag
    37081 ctggttatcc agcgcgaggg taaaattcac cgtcagatct acgaacacgg tgtaccgcag
    37141 gccccgctgg cggttaccgg cgagactgaa aaaaccggca ccatggtgcg tttctggccc
    37201 agcctcgaaa ccttcaccaa tgtgaccgag ttcgaatatg aaattctggc gaaacgtctg
    37261 cgtgagttgt cgttcctcaa ctccggcgtt tccattcgtc tgcgcgacaa gcgcgacggc
    37321 aaagaagacc acttccacta tgaaggcggc atcaaggcgt tcgttgaata tctgaacaag
    37381 aacaaaacgc cgatccaccc gaatatcttc tacttctcca ctgaaaaaga cggtattggc
    37441 gtcgaagtgg cgttgcagtg gaacgatggc ttccaggaaa acatctactg ctttaccaac
    37501 aacattccgc agcgtgacgg cggtactcac ctggcaggct tccgtgcggc gatgacccgt
    37561 accctgaacg cctacatgga caaagaaggc tacagcaaaa aagccaaagt cagcgccacc
    37621 ggtgacgatg cgcgtgaagg cctgattgcg gtcgtttccg tgaaagtgcc ggacccgaaa
    37681 ttctcctccc agaccaaaga caaactggtt tcttctgagg tgaaatcggc ggttgaacag
    37741 cagatgaacg aactgctggc agaatacctg ctggaaaacc caaccgacgc gaaaatcgtg
    37801 gttggcaaaa tcatcgatgc tgcccgtgcc cgtgaagctg cgcgtcgtgc gcgtgaaatg
    37861 acccgccgta aaggtgcgct cgacttagcg ggcctgccgg gcaaactggc agactgccag
    37921 gaacgcgatc cggcgctttc cgaactgtac ctggtggaag gggactccgc gggcggctct
    37981 gcgaagcagg ggcgtaaccg caagaaccag gcgattctgc cgctgaaggg taaaatcctc
    38041 aacgtcgaga aagcgcgctt cgataagatg ctctcttctc aggaagtggc gacgcttatc
    38101 accgcgcttg gctgtggtat cggtcgtgac gagtacaacc cggacaaact gcgttatcac
    38161 agcatcatca tcatgaccga tgcggacgtc gacggctcgc acattcgtac gctgctgttg
    38221 accttcttct atcgtcagat gccggaaatc gttgaacgcg gtcacgtcta catcgctcag
    38281 ccgccgctgt acaaagtgaa gaaaggcaag caggaacagt acattaaaga cgacgaagcg
    38341 atggatcagt accagatctc tatcgcgctg gatggcgcaa cgctgcacac caacgccagc
    38401 gcaccggcat tggctggcga agcgttagag aaattggtgt ctgagtacaa cgcgacgcag
    38461 aaaatgatca accgcatgga gcgtcgttat ccgaaagcaa tgctgaaaga gcttatctat
    38521 cagccgacgc tgacggaagc cgacctctct gatgagcaga ccgttacccg ctgggtgaac
    38581 gcgctggtca gcgaactgaa cgacaaagaa cagcacggca gccagtggaa gtttgatgtc
    38641 cacaccaatg ccgaacaaaa cctgtttgaa ccgattgttc gcgtgcgtac ccacggtgtg
    38701 gatactgact atccgctgga tcacgagttt atcaccggtg gcgaatatcg tcgtatctgc
    38761 acgctgggtg agaaactgcg tggcttgctg gaagaagatg cgtttatcga acgtggcgag
    38821 cgtcgtcagc cggtagccag cttcgagcag gcgctggact ggctggtgaa agagtcccgt
    38881 cgcggcctct ccatccagcg ttataaaggt ctgggcgaga tgaacccgga acagctgtgg
    38941 gaaaccacca tggacccgga aagccgtcgt atgctgcgcg ttaccgttaa agatgcgatt
    39001 gctgccgacc agttgttcac cacgctgatg ggcgacgccg ttgaaccgcg ccgtgcgttt
    39061 attgaagaga acgccctgaa agcggcgaat atcgatattt aatggcgcta accatgcgag
    39121 cgtgcctgat gcgctacgct tatcaggcct acgaaaatct tgcaatgtat tgaattttca
    39181 tgattttgta ggccggataa ggcgttcacg ccgcatccgg caagaagcta taagaaaagg
    39241 gcggagatca tctccgccct ttttatttct gcaatccgca attatcatcc cgcaaatcta
    39301 tactttttgg gtcaacacgt aacgctttac aggtgattat catggggctt tttgatgaag
    39361 ttgtcggtgc ctttctgaaa ggcgatgcgg ggaaatatca ggctatttta agttgggttg
    39421 aggagcaggg cggcattcag gtgctgctgg aaaaactgca aagtggcggc ttaggggcca
    39481 ttctctcaac ctggctgagt aatcaacagg gcaatcaatc ggttagtggc gagcaactgg
    39541 aatcggcgct cggcacaaat gcggtgtccg atctcgggca aaaacttggc gtggatacca
    39601 gtacagcttc cagtttactg gcagagcaat tgccgaagat tattgatgcg ctctcaccgc
    39661 aaggtgaagt gtcaccacaa gccaataacg atctgctttc cgcaggtatg gaactgctga
    39721 aagggaaact cttccgctaa gcaaatgggg agcatgcaac ccgcgttgca tccccctttt
    39781 ccctcgggtg ttgtactgat ttttgagcgg aatcgcgtta gcatgggtca ggaaccaatc
    39841 tacctgggga actcatggct atcaaactca ttgctatcga tatggatggc acccttctgc
    39901 tgcccgatca caccatttca cccgccgtta aaaatgcgat tgccgcagct cgcgcccgtg
    39961 gcgtgaatgt cgtgctaacg acgggtcgcc cgtatgcagg tgtgcacaac tacctgaaag
    40021 agctgcatat ggaacagccg ggcgactact gcattactta taacggcgcg ctggtacaga
    40081 aggccgctga tggtagcacc gtggcgcaaa ctgctctcag ctatgacgac tatcgtttcc
    40141 tggaaaaact ctctcgcgaa gtcggttctc atttccacgc cctggaccgc accacgctgt
    40201 acaccgccaa ccgtgatatc agctactaca cggtgcatga atccttcgtt gccaccattc
    40261 cgctggtgtt ctgcgaagcg gagaaaatgg accccaatac ccagttcctg aaagtgatga
    40321 tgattgatga acccgccatc ctcgaccagg ctatcgcgcg tattccgcag gaagtgaaag
    40381 agaaatatac cgtgctgaaa agtgcgccgt acttcctcga aatcctcgat aaacgcgtta
    40441 acaaaggtac gggggtgaaa tcactggccg acgtgttagg tattaaaccg gaagaaatca
    40501 tggcgattgg cgatcaggaa aacgatatcg caatgattga atatgcaggc gtcggtgtgg
    40561 cgatggataa cgctattcct tcggtgaaag aagtggcgaa ctttgtcacc aaatccaacc
    40621 ttgaagatgg cgtggcgttt gctattgaga agtatgtgct gaattaatca gtggacgggc
    40681 aaacagcata aacgcgtttg cccgcttact gattattggt ttactgcata tacaaccaga
    40741 aattgcagcg gtgcagtaat gatgtccagc gtaacagtca ctggcagtaa tgctgcggaa
    40801 acaccatccg gaatgcctct gggtgaatag tattcataaa atcgcacttt aaacggatga
    40861 tagaacgcca tcacctttga gtaatccatg tttttatttt tctcatggat tgtgcctttt
    40921 aggttttcca gtgacctggt acagtgttga acaccacagg caaaaccata atgcgttgcc
    40981 agtgcctttt cttcttcgtt attccagtag tagtaaagcg atattgtgcc ggtaaatttc
    41041 tttttccccg catttaaaac aaatcttgcg tcatcggcaa cctgaatatt gtgccggtta
    41101 agtgctggat cattgagcat cttaacgatg tcatcggcac catcagtgat aaggtaatcc
    41161 agcgattccc ctacaaaaac atagcctttt gttccattac tatcttttgc ctgcgatagc
    41221 ccagtgatgg tgtctgactg cgattgatac tttcctgaac taccctttgc ggtatgggaa
    41281 accagagcat acgagataca gcctgaaagc atcagcgcca gtgggaataa accggcagcc
    41341 ttaaaaaatc ccttaaaatt caacttcatg attaatcctt taaacgagta gctaagccgt
    41401 tgaaagcata ctcttctttc cctcctgttt agcgcctgat atcccttttc agatttctgc
    41461 ccgacgcatg tcattttttt atgcattgtt ctttttgtga tctaaattgt agtacaacaa
    41521 tataagtttg tactacatta cgcacacggc aaacgcgaac gtcatcacgc tggtactaca
    41581 aagttgccgc gttatgcatc gatcggggta aagtagagaa gaacatacag agcacaagga
    41641 ctctccatga ctctcaataa aaccgatcgc attgtcatta cgctgggtaa acagatcgtt
    41701 cacggcaaat acgtacctgg ctcgccactt ccggctgagg cggagctctg tgaagagttt
    41761 gcaacctcgc gcaacatcat ccgtgaggtg ttccgttcgt tgatggcgaa gcggctgatt
    41821 gaaatgaaac gttatcgcgg cgcgtttgtg gcaccgcgta accagtggaa ttacctcgac
    41881 actgacgtac tgcaatgggt gctggaaaac gactacgacc cacggcttat cagtgccatg
    41941 agcgaagtgc gaaatctggt ggaaccggcg attgcccgtt gggcagcaga gcgcgcgacc
    42001 tccagcgatc tggcgcagat tgaatcggcg ctgaacgaga tgattgccaa caatcaggac
    42061 cgcgaagcgt ttaacgaagc ggatattcgc taccacgagg cggtgctgca gtcggtgcat
    42121 aacccggtgt tacagcaact tagcattgcg atcagttcgc tgcagcgggc ggtttttgaa
    42181 cgaacctgga tgggcgatga ggccaacatg ccgcaaacgc tccaggaaca taaggcgctg
    42241 ttcgatgcga tacgacatca ggacggcgat gcggcagagc aggcggcgct taccatgatc
    42301 gccagctcga cacgaaggtt aaaggaaatc acatgacagc tcgctacatc gcaattgact
    42361 ggggatcgac caatctgcgt gcctggcttt atcagggcga ccactgcctg gacagcaggc
    42421 aatcagaagc aggcgtcacg cgcctgaacg gaaaatctcc ggctgcggtg ttagcagaag
    42481 tcacgaccga ctggcgtgaa gagaatacgc cagtggtgat ggcaggaatg gtcggcagca
    42541 atgtcggctg gaaagttgca ccgtatttat ctgttcctgc ccgtttttcg tctattggcg
    42601 atcaattaac gtcggttggc gacaatatct ggattattcc cggattatgc gtctctcatg
    42661 acgataacca caatgtgatg cgcggcgaag aaacacaatt gatcggcgcg cgcactctgg
    42721 ctccttcctc tctttatgtc atgcccggaa cccattgcaa atgggtgcag gccgatagcc
    42781 agcaaatcaa cgattttcgc accgtgatga ccggtgaatt acatcattta ctgttaaatc
    42841 actcattgat tggcgcaggt ttgccgccgc aggaaaactc tgccgatgcc ttcgcggctg
    42901 gccttgagcg tggccttaat gcgcccgcca tattgccgca gctttttgaa gttcgcgcct
    42961 cgcatgtgct gggaacactt ccccgcgaac aggtcagcga atttctctct ggtttgttga
    43021 ttggcgcaga ggtcgccagt atgcgcgact atgtggccca tcaacacgcc atcacccttg
    43081 tcgccggaac atcgctgacc gcgcgctacc agcaagcctt tcaggcgatg ggttgcgacg
    43141 tgacagcggt agcaggcgac acggcatttc aggctggtat aaggagcatc gctcatgcag
    43201 tggcaaacta aactcccgct gatcgccatt ttgcgcggca ttaagcccga cgaggcgctg
    43261 gcgcatgttg gcgcggtgat tgacgccggg ttcgacgcgg ttgaaatccc gctgaattcc
    43321 ccacaatggg agcaaagcat tcccgccatc gttgatgcgt atggcgacaa ggcgttgatt
    43381 ggcgcaggta cggtactgaa acctgaacag gtcgatgcgc tcgccaggat gggctgtcag
    43441 ctcatcgtta cgcccaatat ccatagtgaa gtgatccgcc gtgcggtggg ctacggcatg
    43501 accgtctgcc ccggctgcgc gacggcgacc gaagccttta ccgcgctcga agcgggcgcg
    43561 caggcgctga aaatatttcc gtcatcggct tttggtccgc aatacatcaa agcgttaaaa
    43621 gcggtattgc catcggacat cgcagtcttt gccgttggcg gcgtgacgcc agaaaacctg
    43681 gcgcagtgga tagacgcagg ttgtgctggg gcgggcttag gcagcgatct ctatcgcgcc
    43741 gggcagtccg tagaacgcac cgcgcagcag gcagcagcat ttgttaaggc gtatcgagag
    43801 gcagtgcaat gaaaatcacc aaaattacca cgtatcgttt acctccccgc tggatgttcc
    43861 tgaaaattga aaccgatgaa ggcgtggtcg gttggggcga gcccgtgatt gaaggccgcg
    43921 cccgtacggt ggaagcggca gttcacgagc tgggtgacta tttgattggt caggatcctt
    43981 cgcgcatcaa tgacttatgg caagtgatgt atcgcgccgg attttatcgt ggcggtccaa
    44041 tcctgatgag cgccattgcc gggatcgacc aggcgttatg ggatatcaaa ggtaaagtgc
    44101 tgaatgcgcc ggtctggcaa ctgatgggcg gcctggttcg cgacaaaatt aaagcctaca
    44161 gttgggtcgg cggcgatcgt ccggcggatg tgatcgacgg tattaaaacc ctgcgcgaaa
    44221 tcggcttcga taccttcaaa ctgaacggtt gtgaagaact ggggctaatt gataactccc
    44281 gcgcggtaga tgcggcagtc aacaccgtgg cacaaattcg tgaagctttt ggcaatcaga
    44341 ttgagtttgg tcttgatttc cacggtcgcg tcagcgcgcc aatggcgaaa gtgctgatta
    44401 aagaactgga gccgtatcgc ccgctgttta ttgaagagcc ggtgctggcg gaacaagccg
    44461 aatactaccc gaaattggcg gcacaaacgc atattccact ggcggcgggt gagcgcatgt
    44521 tctcacgttt cgattttaaa cgcgtgctgg aggcaggcgg tatttcgatt ctacaaccgg
    44581 atctctccca tgcaggcggt attaccgaat gctacaaaat tgccggaatg gcagaagcct
    44641 atgacgtgac ccttgcgccg cactgtccgc tcggaccgat tgcactggcg gcttgtctgc
    44701 atatcgactt tgtttcctat aacgcggtac ttcaggaaca aagtatggga attcattaca
    44761 acaaaggcgc ggagttactc gactttgtga aaaacaaaga agacttcagc atggttggcg
    44821 gcttctttaa accgctaacg aaaccgggct taggcgtgga aatcgacgaa gctaaagtta
    44881 ttgagttcag taaaaatgcc ccggactggc gtaatccgct ctggcgtcat gtagataaca
    44941 gcgtagcaga gtggtaattc ctgccacgta agcccctcat cgggcactaa aacagcaata
    45001 caaaaatata accctctgta aattacaggg catggtgagc ggcttcgcta tgcccaaaat
    45061 ctggagaaag ctcgctatgg atattcccgt taatgcagca aagccggggc gtcggcgtta
    45121 tctgacgctg gtgatgatct ttattacggt ggtcatttgt tatgttgacc gcgctaacct
    45181 ggccgtggct tccgcccata ttcaggaaga gttcggcatt acgaaagcgg aaatgggcta
    45241 tgtattttcg gccttcgcct ggctttatac gctatgccag atccccggcg gttggttttt
    45301 agatcgcgta ggttctcgcg tgacttatta tattgcgata tttggttggt cagtggcgac
    45361 tttattccag ggctttgcca cgggattaat gtcattaatt ggtctgcgtg cgataaccgg
    45421 tattttcgaa gcgcccgctt tcccgaccaa taaccggatg gtgaccagct ggttcccgga
    45481 acatgaacgc gcttccgccg ttggttttta tacgtctggt cagtttgtcg gtctggcgtt
    45541 tttgactccg ctgctgatct ggattcagga gatgttgagc tggcactggg tgttcattgt
    45601 cactggtggt atcggcatta tctggtcgct gatttggttt aaggtttatc agccgccgcg
    45661 cctgaccaaa ggtatcagca aagctgaact ggattacatt cgtgatggcg gcggtctggt
    45721 ggatggtgat gcgccggtga agaaagaggc gcgtcagccg ttaacagcca aagactggaa
    45781 actggtgttc catcgtaaac tgatcggcgt ttatcttggg caatttgcgg tggcttctac
    45841 actgtggttt ttcttaacct ggttcccgaa ctatttaacc caggaaaaag gaatcacggc
    45901 gctgaaagca ggctttatga ccacggtgcc attcctcgcg gcgtttgtcg gcgtcctgct
    45961 ctctggctgg gtagcggatc tgctggtacg taagggcttt tcactgggct ttgcgcgtaa
    46021 aacgccgatt atctgcggct tgctgatctc cacctgcatt atgggcgcta actacactaa
    46081 cgatccgatg atgattatgt gcctgatggc gctggcattc ttcggtaacg gttttgcttc
    46141 gattacctgg tcgctggttt cttctctggc accgatgcgc ctgattggtt taaccggcgg
    46201 cgtgtttaac ttcgccggtg gtctgggcgg catcaccgtt ccgctggtgg tggggtacct
    46261 ggcgcagggt tacggtttcg cacctgcact ggtttatatc tccgccgtcg cgttgattgg
    46321 cgcgctctct tatatcctgc tggtgggcga tgtgaagcgc gttggctaat ccttcaactg
    46381 tggaatatgt gccacaccac tgcgcataat ccactttctt aaagccggtg cggtcaggca
    46441 tcggcttttt actatcttgc cgaagagttt taaacgcagt ttgcgggtgg cgcgccagta
    46501 agcggtattg agcttctctg gctgcttcag taacaccgaa cgcagaatgt ctgcgctatc
    46561 cagcgcatag ctaatcccct ccagcgagct ggcgctgata aatcccgccg cttcaccaat
    46621 cagaaaggcg ttgtccttac cgcagacaaa atcctgccag cgtgagggaa acagcaccgt
    46681 gcatttttcg cttttcaccg ccttaccaaa ctggaactga aaggcgctca ttttctcttt
    46741 cagcgtcgtg aaacgcgtct gaccgtcttt cattggataa gcaccgccaa agataaaata
    46801 accgtctttg ctgatactcc atgaataaca gttagttatc gaattatcaa agatgcagga
    46861 gtagaacggc accggatgtt tctccgcgaa ccactgctgg atagcgacat atttacggat
    46921 ttgatgatcc gggtagagat gtcgccgcac catcgagttt gcgccatcgg caccgaccag
    46981 atagcgggcg gtaatatgtt gctcccagcc gtctgcacga aaaatgacat gccatttatc
    47041 atcctcacgc cagatttttc ggcacaggct atcgtgataa acctcaacgc tggtggggat
    47101 cagcgatttc atccacaagt cgaaagcatg gcgattaata ttgatatagc ttcgctggta
    47161 gttacgcgtg agcgatgcgg cgacgtcgac agttttgacg ctgaaaatct gtggattggc
    47221 gatcacatcg acaggaagcg tcagtccatc gcgaataaaa gatcgctgcg cgtccggtgc
    47281 cagcagaccg ccgcaaggtt tgctgaaacc ttcagtacca cactggtgct ttttatccag
    47341 cgcgatcacc tgcattttgc ctgctaattt tcgcgccaac gccgatcctg ccgggccgag
    47401 gccaataatc gccacgtcga aatgttccat cttttcactc acattcatca cgatggcaaa
    47461 agactattgt ctgaaagtga agtccttgtg aagtatgatg gcgggacccg ttttgtatca
    47521 ggaccgcgcc atgaaacaga taacctttgc tccccgtaat cacctgctca ccaataccaa
    47581 tacctggacg cccgacagcc agtggctggt atttgacgtg cgtccttctg gggcgtcgtt
    47641 taccggcgag accattgagc gtgtgaatat ccataccggc gaggtcgagg ttatctatcg
    47701 cgcgtcacag ggcgcacacg tcggcgtggt gaccgttcat ccgaagtcag agaaatatgt
    47761 tttcatccat ggcccggaaa atcctgatga aacatggcat tacgatttcc atcatcgacg
    47821 cggagtcatt gctgaaggcg gcaaggtgag caatctcgat gcaatggata ttaccgcgcc
    47881 gtatacgcca ggagcgctgc gcggcggcag ccatgtgcat gtctttagcc cgaacggtga
    47941 aagggtgagc tttacctata acgaccatgt aatgcatgaa ctcgatccgg cgctggattt
    48001 gcgaaacgtc ggtgttgctg cgccgtttgg cctggtcaac gtacaaaagc agcatccgcg
    48061 tgaatacagc ggtagccact ggtgcgtgct ggtgagtaaa accacgccca cgccgcagcc
    48121 tggcagcgat gaaatcaatc gtgcttatga agaaggatgg gtaggaaatc acgcgctggc
    48181 gtttattggc gacacacttt cgccaaaggg cgagaaagtg ccggagctgt ttatcgttga
    48241 gttaccgcaa gatgaagctg gctggaaagc ggcaggtgat gcgccgttaa gtggaacgga
    48301 aaccaccctg cccgcgccac cgcgcggcgt cgtgcagcga cgtttaacct ttacccacca
    48361 tcgggcttat ccggggttag tcaacgtccc gcgccactgg gtgcgctgta atccgcaggg
    48421 tacgcaaatc gcgtttttaa tgcgtgatga taacggcatt gtgcaactgt ggcttatctc
    48481 gccacagggc ggcgagccgc gccagttaac ccataacaaa acggatattc agtctgcatt
    48541 taactggcat ccgtcaggag aatggttggg ctttgtgctg gataatcgaa ttgcttgtgc
    48601 ccatgcgcaa agcggcgagg ttgagtattt aaccgaaaac cacgccaatc caccttctgc
    48661 ggacgccgtg gtcttctcgc cggatggtca gtggctggcg tggatggaag gcggccagct
    48721 gtggatcacc gaaactgatc gctaaatcag ttatcaggca ttctggccgg tggaataacc
    48781 gcattggtgg cttgcgcgtt ggcttcgctt ttttcaacgc gtgagcgcac cgaactgtct
    48841 ttgcggaaca catcccacgg cagtaaaagc gtgtccatca cagcggtaaa cggcatatcg
    48901 agaatcgcca gcgacttagt gccccagttg gtttcatcgt cgccaatcat ggttgcgctg
    48961 gcgcgggtgc cgggatacgt tccttctttc cctccggtat gggacatcac gctggagcat
    49021 cctccgagta atgtcattcc gctgcatatc atgaacgcta acaacacatt tcttatcata
    49081 ataaattcat ctgttgatcg tgggtgttgg cctgatgagt tatagcgatc ccttgctgaa
    49141 aataacatca tcattacgtc gcactgtggc ggctatcgca ctttaacgtt tcgtgctgcc
    49201 ccctcagtct atgcaataga ccataaactg caaaaaaaag tccgctgata aggcttgaaa
    49261 agttcatttc cagacccatt tttacatcgt agccgatgag gacgcgcctg atgggtgttc
    49321 tggctacctg acctgtccat tgtggaaggt cttacattct cgctgatttc aggagctatt
    49381 gattatgcgt aactttgatt tatccccgct ttaccgttct gctattggat ttgaccgttt
    49441 gtttaaccac ttagaaaaca accagagcca gagtaatggc ggctaccctc cgtataacgt
    49501 tgaactggta gacgaaaacc attaccgcat tgctatcgct gtggctggtt ttgctgagag
    49561 cgaactggaa attaccgccc aggataatct gctggtggtg aaaggtgctc acgccgacga
    49621 acaaaaagag cgcacctatc tgtaccaggg catcgctgaa cgcaactttg aacgcaaatt
    49681 ccagttagct gagaacattc atgttcgtgg cgctaacctg gtaaatggtt tgctgtatat
    49741 cgatctcgaa cgcgtgattc cggaagcgaa aaaaccgcgc cgtatcgaaa tcaactaatt
    49801 ccttaaggcc gcctggcgcg gcctgacatc tccatgctcg ccgtcaggga gcatatgcga
    49861 atcttcggat ttgcaggtac ttactcgctt cttagaagga gaaatgacta tgcgtaactt
    49921 cgatttatcc ccactgatgc gtcaatggat cggttttgac aaactggcca acgcactgca
    49981 aaacgccggt gaaagccaga gcttcccgcc gtacaacatt gagaaaagcg acgataacca
    50041 ctaccgcatt acccttgcgc tggcaggttt ccgtcaggaa gatttagaga ttcaactgga
    50101 aggtacgcgc ctgagcgtaa aaggcacgcc ggagcagcca aaagaagaga aaaaatggct
    50161 gcatcaaggg cttatgaatc agccatttag cctgagcttt acgctggctg aaaatatgga
    50221 agtctctggc gcaaccttcg taaacggttt actgcatatt gatttaattc gtaatgagcc
    50281 tgaacccatc gcagcgcagc gtatcgctat cagcgaacgt cccgcgttaa atagctaact
    50341 agctattctc tttgccccgc cattctggtg gggctttttt tgtctttctt cttcaacaat
    50401 gcgttaaatt tcgcctgttt aagatatttc ggcacgttct ttcccgtgat aatgtgcgct
    50461 ataaggcaaa tctgcttcac cgattcagcc ataatccgta gcaattaaaa atgttatctc
    50521 aagagaaatg gacgatgagt gatatagcat taacggtcag tattctggct ttggtggcag
    50581 tcgtcggttt gtttatcggc aatgtcaaat ttcgcggcat aggattaggt attggcggcg
    50641 tgctgtttgg tgggatcatc gtcggccatt ttgtttctca ggcggggatg acattaagta
    50701 gcgatatgct gcatgttatt caggaatttg gcctgatcct gttcgtttat actatcggga
    50761 ttcaggtagg gccgggcttc tttgcctcat tgcgcgtctc cggattacgc ctcaacctgt
    50821 ttgctgttct gatcgtcatc atcggtggtc tggttaccgc catcctgcat aaactgtttg
    50881 atattccact gccggtagtg ctggggattt tctccggtgc ggttaccaat acgccagcgc
    50941 tgggggcagg gcagcagatt ttgcgcgacc tgggtacacc aatggaaatg gtcgatcaga
    51001 tggggatgag ttacgcgatg gcgtatccat tcggcatttg cgggattttg ttcaccatgt
    51061 ggatgttgcg ggttattttc cgcgtcaatg tcgagacaga agctcagcag cacgagtctt
    51121 cacgcaccaa tggcggcgcg ctgatcaaga ctatcaatat tcgcgttgag aaccctaacc
    51181 tgcatgattt agccattaaa gatgtaccga ttctcaacgg cgacaaaatt atctgctcgc
    51241 gtctgaaacg cgaagaaacc ctaaaagttc cttcgccaga taccattatt caactgggcg
    51301 atttgctgca tctggtgggt cagccagcgg atttacataa tgcgcaactg gtgattggtc
    51361 aggaggtcga tacttcgctg tccacgaaag gcactgattt gcgcgtcgag cgtgtggtgg
    51421 tcaccaatga aaacgtgctc ggaaaacgta ttcgcgacct gcactttaaa gaacgctatg
    51481 acgttgttat ctcgcgcctg aaccgtgccg gggtcgaact ggtcgccagt ggcgatatca
    51541 gcctgcagtt cggcgatatc ctcaacctgg tggggcgtcc gtccgcaatt gatgccgttg
    51601 ccaatgtgct ggggaatgcg cagcaaaaac tgcaacaggt tcagatgctg ccagtgttta
    51661 ttggcatcgg gcttggcgta ttgttaggtt ctattcccgt ctttgtgcca ggattcccgg
    51721 ccgcgttgaa actggggctg gcgggcggtc cgctgattat ggcgttgatc ctcgggcgta
    51781 tcggcagtat cggcaagctg tactggttta tgccgccaag tgccaacctc gcgctgcggg
    51841 agctggggat cgtgctgttc ctctcggtcg ttggtctgaa atctggtggg gattttgtga
    51901 ataccctggt caatggcgaa gggctaagct ggattggtta tggtgccctg atcaccgccg
    51961 ttccgctgat tactgttggc attctggcgc ggatgttagc caaaatgaat tacctgacca
    52021 tgtgcgggat gctggcaggt tccatgaccg atcctccggc gctggcgttt gctaataatc
    52081 ttcatccaac cagtggtgcg gcggcgctct cttacgccac tgtctatccg ttagtgatgt
    52141 ttctgcgaat tatcaccccc caattactgg cggtgctctt ctggagtatc ggttaactct
    52201 tttccgggtg gagtcgccgt aaatggtact ccacctggta ttcgctggca tttctgaaca
    52261 tcactgaata attcaaaaac tccccgctct cgctatatga caacgaggtg atccgcagca
    52321 gtggcgtgtg ttccgctacc ttcatttgcc gcgccagcag tctatcggcg agcaccggcg
    52381 tcaggctttc ataattaccg ccaatcaaaa tcccgcactc ttgttcaata tattcaaact
    52441 tcgacccttc cagatgttgc agcgaaaggt tacggaacag ttttaccggc atatagctgt
    52501 cttccagcat cagcggtttc ccttccacaa aacgaacgcg acgggagaag tagatctgct
    52561 cgttgatttg aatccgtaac tggctggcaa tgaccggagg cgcaggcatg atttcaaaaa
    52621 tcagcacctg gctggtgacc gtttttccct gccgttttaa cacctccacc agcccggtca
    52681 ggctggcggt ttgatgcagt acatctttgc gcaccaggta agtgccgctg ccgtggcggc
    52741 ggaccaccaa tccccacgct accagcaggt caatggcttt acggatggtc atccgcgata
    52801 ccgcaaattc ttccgccaac tttttttcac cgggaagaag gctatttagc gtgaaatctg
    52861 cggaattaag tcgaattctt aaccgctccg caatgctttt gtagatcatc tctagatctc
    52921 ttttttgcct gcaagcccgg gtaatgcgcc cacaagttct gcttaatcga ttgaaaaaac
    52981 atcttttttt taaagatgtg ttcgatgctg tgtacctgtg ctcacagatg tctacttttt
    53041 cgcgaaaacg tagatctcta ccgcccaacg aaaagcatga aagcgatcac gaatcccatt
    53101 gggtcgtcat gttctgccga tcgcatcttc ctatcctcgc tccaggcctg ccgcataacc
    53161 aatcaggctt cctacttaca gaattgagaa aagaggatgt ggaaatgctc agtcaaattc
    53221 aacgctttgg cggcgcgatg ttcacgccag tgctgctgtt tcccttcgcc gggattgtgg
    53281 tgggtcttgc catcttgctg caaaacccga tgtttgtcgg ggaatcactg accgatccga
    53341 acagtttatt cgcgcaaatc gtacacatta ttgaagaggg cggttggacg gtattccgta
    53401 atatgccgct gatttttgct gtcggtttac ccattggcct tgctaagcaa gcgcagggac
    53461 gtgcttgtct ggcggtgatg gtgagtttcc tgacctggaa ctatttcatc aatgcgatgg
    53521 gaatgacctg gggaagctac ttcggcgtcg atttcactca ggacgcggtg gcaggtagcg
    53581 gtctgacaat gatggctggg attaaaaccc tcgataccag cattatcggc gcaattatca
    53641 tttccggcat tgtgacggcg ctgcataacc gtctgttcga taaaaaactg ccggtgtttc
    53701 tcggcatttt ccaggggacg tcttatgtgg tgattatcgc cttcctggtg atgatcccct
    53761 gtgcctggct gacattgctc ggctggccaa aagtacaaat ggggattgaa tctctgcaag
    53821 cgttcctgcg ttcggcgggt gcgcttgggg tgtgggttta caccttcctc gaacgtattc
    53881 tgatcccaac cggtttacac cacttcatct acggaccgtt tatctttggt ccggcagctg
    53941 ttgaaggcgg cattcagatg tactgggcgc agcatctgca agagttcagt ctgagcgccg
    54001 agccgctgaa atcgttgttc ccggaaggcg gttttgccct gcacggtaac tcaaaaatct
    54061 ttggtgccgt gggcatttct ttagcgatgt acttcactgc cgcaccggaa aatcgggtaa
    54121 aagtggcggg cttgctgatt cccgcaacct taaccgccat gctggtggga attaccgaac
    54181 cgctggaatt taccttcctg ttcatttcac cgttgctgtt tgcggtacac gccgtgctgg
    54241 cggcctcaat gtcgaccgta atgtatctct ttggtgtggt gggcaacatg ggcggaggtc
    54301 tgattgacca ggttttaccg caaaactgga tcccgatgtt cagcaaccac gcggatatga
    54361 tgctgaccca aatcgccatt gggttgtgct ttaccctgct gtacttcgtg gttttccgca
    54421 cactgattct gcagttcaac atgtgcacgc cgggacgtga agatgcggaa gtgaaactct
    54481 actcaaaagc cgaatacaaa gcctcgcgag gccaaaccac cgcggcagag ccaaaaaaag
    54541 agctggatca ggctgccggt atcctgcaag ccctgggcgg ggtcggcaat atctccagca
    54601 ttaacaattg cgcgacgcgt ttacgcattg cactgcatga catgtcacaa acgctggatg
    54661 acgaagcctt taaaaagctg ggagcgcacg gcgtcttccg tagtggcgat gccattcagg
    54721 tgatcattgg tctgcatgta tcccagctgc gtgaacagct cgatagctta attaattctc
    54781 atcaatcagc agaaaatgtt gccattacgg aggcagtata atgaccaaat tctcagtggt
    54841 tgtcgcaggc ggtggaagca cctttacgcc aggcatcgtg ttgatgctcc tggcgaatca
    54901 ggagcgtttc ccgcttcgtg cactgaaatt ttatgataac gatggcgcgc ggcaggaagt
    54961 gattgccgaa gcctgtaaag tcatccttaa agagaaagcg ccggacattg cgtttagtta
    55021 caccaccgat cctgaagtgg cgttcagcga cgttgatttt gtcatggcgc acatccgcgt
    55081 cggcaagtac ccgatgcgcg aactggatga aaaaatccca ctgcgccacg gtgttgttgg
    55141 tcaggaaact tgcggacccg gcggaatagc gtacggcatg cgttccattg gcggcgtcct
    55201 ggaactggtg gattatatgg aaaaatattc accaaatgcc tggatgctca actactccaa
    55261 cccggcagcc attgtcgcag aagccacgcg tcgtctgcgc ccgaatgcga aaatcctcaa
    55321 catctgtgac atgccaatcg gtattgaaag ccggatggcg caaattgttg ggctgcaaga
    55381 tcgcaaacag atgcgcgtgc gctactacgg cctgaaccac tggtggtcgg cgatatcccg
    55441 cagtttcaga aagggttaat gagtcagcaa gtggcggtgg aaaaactggt cgtggacgcc
    55501 tgggaacagc gttcatatca gcacctgtgg caggcgatta cgttgtcgaa aacggtaccg
    55561 agcgcctcgg tcgccaaagc tattctggat gagttgctgg aggccaacaa agcgtactgg
    55621 ccagagttac gttaattgtg tggactggca tccgttgggt gctggtccgt attctttagc
    55681 acggctgacg gcgcataacc aaatttatgt ttaaaggctt tgctgaaatg aaaggagtcg
    55741 aagaagttga gcgtatcagc gacttcgcca accgagtttc cttgttgctt taatagcgat
    55801 agcgccagat ccagacgcgc atcgaggtaa tattctttcg gcgtcttgcc ggtatagcgt
    55861 aaaaacagac gacgcaacca ggcttcgcta caggggatcg tggcagccat atcagctacg
    55921 ctccagcgtt gttgcagact ggcatgtaaa gtggcaatta atttttcaat ttgccgccgt
    55981 tgtggatctt ttttaccatc tgcgtacata agacaaatcc actgataaat aattttcgtc
    56041 agaaaagcga ctgccagatt attttttatt gcttctggtg aagttattaa ctcagcaact
    56101 tcggtgagtt cctgattata aatttcgcca ttataaataa cgctttgctg accaacggga
    56161 atatccatca tactggtggg ggtaaattcc atccagtact gttcccagac taaaccttca
    56221 cagtgataag agtgaatatc cattggcgtt aagaatataa tacagttgcc atgcagtgtt
    56281 atttgttcgc cattctttaa aagaattttc ccacatcctt gtacggtata caccgcaacc
    56341 catgagttgg cgataagcgg tttcttcttg tcccgcggcc agatcacccg gtaatgctcg
    56401 tcggcgtagg tatgccagag cgagatcaga gaaattccgc tggagatcac attttcgtta
    56461 atcaataagg gaatattgta cggagtttcg ttttttacat ctgaactttg caattttcca
    56521 ttcattttaa tatccctccg gcgaattatg ctcacaacct gaacttaaca gaaaacaatc
    56581 cgttcaggga tacgacgttt gtattttaag aatctgactg cctgacccga cgcattttac
    56641 ctctccctat attcatgcgt ccgggacata aacataaata agggctatga gatgaattcg
    56701 ttacaaatct tgagttttgt cggttttacg ctgctggtgg cggtgatcac ctggtggaag
    56761 gtccgcaaaa cagataccgg atcgcaacaa ggctattttc ttgccggacg ttcactaaaa
    56821 gcgccggtta ttgccgcttc gttaatgcta accaaccttt ccacggaaca actggtcggc
    56881 ctttccgggc aggcctacaa aagcggcatg tcggtgatgg gctgggaagt gacttcagcg
    56941 gtgacgctga tcttcctcgc gctaatcttt ttaccgcgct atctgaagcg cggcattgcc
    57001 accatccccg attttctgga ggaacgttat gataaaacga cgcgtattat catcgacttc
    57061 tgcttcctca ttgccaccgg cgtctgcttt ctgccgattg ttctctactc cggcgcgttg
    57121 gcgctcaaca gcctgtttca cgtcggggaa tcgctacaga tttctcacgg tgcggctatc
    57181 tggctactgg taattttgct tggtctggcg ggaattttgt atgcggtgat cggcggactg
    57241 cgcgcaatgg cagtggcgga ctccatcaac ggtattgggc tggttatcgg cgggttgatg
    57301 gtgccggtat ttggcctgat cgcgatgggc aagggcagct ttatgcaggg cattgagcaa
    57361 ctcaccaccg ttcacgccga gaaattaaac tcaatcggta gcccgaccga tcccttgccg
    57421 attggcgcgg catttaccgg tttgattctg gtgaacacct tttactggtg tacaaatcag
    57481 ggcatcgtgc aacgcacgct ggcgtcaaaa agcctggcgg aagggcaaaa gggggcgctg
    57541 ttaacggcgg tgctgaaaat gctcgacccg ctggtactgg tgctgccagg gttgattgcg
    57601 tttcatctgt atcaggattt accgaaagcc gacatggcct acccgacgct ggtcaataac
    57661 gttctgccag tgccaatggt gggtttcttc ggcgcggtgt tatttggtgc ggtgatcagt
    57721 accttcaacg gctttctgaa tagcgccagt acgttattca gtatgggtat ttaccgtcgc
    57781 atcattaacc agaatgccga gccgcagcag ctggtcaccg tcgggcgcaa atttggtttc
    57841 tttatcgcta tcgtttcggt gctggtcgcg ccgtggatcg ccaacgcgcc gcaggggctg
    57901 tatagctgga tgaaacagct caacggcatt tacaacgtgc cgctggttac catcatcatt
    57961 atgggctttt tcttcccgcg catcccggcg ctggcggcaa aagtggcgat ggggattggc
    58021 ataatcagct acatcaccat caactatctg gtgaagttcg acttccattt cctctatgtg
    58081 ctggcctgta cgttctgcat caacgtggtc gtgatgctgg tgatcggttt tatcaaaccg
    58141 cgcgccacgc cgttcacctt caaagatgcg tttgcggtgg acatgaaacc gtggagaaac
    58201 gtcaagatcg cgtcaattgg catcctgttc gcgatgattg gcgtctatgc cgggctggct
    58261 gaattcggcg gctacggtac gcgctggtta gcgatgatca gttatttcat tgccgccgta
    58321 gtgattgtct acctgatttt tgacagctgg cggcatcgtc acgacccagc cgtaaccttt
    58381 actcccgacg cgaaggatag cctatgaaac gccccaattt tctgttcatc atgaccgata
    58441 cccaggccac caatatggtc ggttgctata gcggtaaacc gctgaatacg caaaatattg
    58501 atagtctggc ggcggaaggt attcgcttta attccgccta cacctgttca ccggtttgta
    58561 cgcctgcacg cgccggacta tttaccggta tctacgctaa ccagtccggc ccgtggacca
    58621 acaacgtcgc gccaggcaaa aacatctcca ctatggggcg ctactttaag gatgccggct
    58681 atcacacctg ttacatcggc aaatggcatc tcgacggtca tgactatttc ggcactggcg
    58741 agtgtccgcc ggagtgggac gctgattact ggttcgatgg ggcgaactac cttagcgaac
    58801 tgacggaaaa agagatcagc ctgtggcgca atggcctaaa cagcgttgag gatttacagg
    58861 cgaaccatat cgacgaaacc ttcacctggg cgcaccgcat cagcaatcgg gcggtagatt
    58921 ttctgcaaca gcccgcgcgc gccgaggaac ccttcctgat ggtggtttcg tatgatgagc
    58981 cgcatcaccc gttcacctgt ccggtggagt atttagagaa atacgctgat ttttactacg
    59041 atctgggcga gaaagctcag gatgacctgg cgaacaaacc ggaacatcac cgcttatggg
    59101 cgcaggcgat gccatcgcca gtcggtgatg acgggcttta tcaccatccg ctctattttg
    59161 cctgtaatga ctttgttgat gaccaaatcg gacgggtcat caatgcctta acgccagagc
    59221 aacgtgaaaa tacgtgggtc atttatactt ccgatcacgg cgaaatgatg ggcacacata
    59281 agctgatcag taaaggagcg gcgatgtatg acgacatcac ccgtattccg ctgatcatcc
    59341 gttcgccgca aggggagcgg cgacaggtcg atacgccagt cagtcatatc gatttactgc
    59401 cgacaatgat ggcgctggca gatattgaaa aaccagagat tctgccgggg gaaaatatcc
    59461 ttgccgtgaa agagccacgc ggtgtaatgg tggaatttaa ccgctacgag attgagcatg
    59521 acagctttgg cggttttatt ccggtgcgtt gctgggtgac ggatgacttt aaactggtac
    59581 tcaacctctt caccagtgat gaactttacg atcgccgtaa tgacccaaat gaaatgcata
    59641 acctgatcga tgatatccgt tttgcagacg ttcgcagcaa aatgcatgac gccttattgg
    59701 attacatgga caaaattcgc gatccgttcc gcagttacca atggagcctg cgtccgtggc
    59761 gtaaagatgc actgccgcgc tggatggggg catttcgtcc acgcccacaa gatggctatt
    59821 cgccggtggt acgtgactat gacaccggcc taccgacgca aggagtgaaa gtggaagaga
    59881 aaaaacagaa gttctgacga taatgcccgc tgatgaacac ccggcgggca ttattcaggc
    59941 tgcttaaacc agtgaaccgc cggtcaggaa ggaaaaaatc aggaatatca gcaaaatgct
    60001 gcacacgatg ccgataccaa aaagcaaggt atggaaagcc agcgtcccgc cgccaaaaac
    60061 cagcgcgccg cgaatgcccc agcgattttc cttacgcata ccgccaataa atgcgcaaag
    60121 tagcgcagca atggccagtg cgataccgct ttgatcgagg attttatcga catctacgtc
    60181 ctcttttttc tccacggtgg taattttttc gccttttatt ccggcgagaa gacctctttt
    60241 aatttcagcc gttttgtccg ccacgatgct ttcaaacgtt ggcggtggtg cggaaaaagg
    60301 gccgaatgaa aagtggataa tccccatcat taaagcgatg gcaccgaaca gcatgcccaa
    60361 tgaactgatt ttattttgag ctatgatacc catctgacct ccttgtagcg gatggcgaaa
    60421 gcatatcagg aaacgggcgg acgaaattct taaaaaaaaa ccgctaaagg tagagcgtta
    60481 gtgaaattgc aaaggagcaa caatgaaaat ttcccgcctt ggagaagcgc ccgattaccg
    60541 cttctcgctg gctaatgagc gtacttttct ggcgtggatc cgtaccgcac taggatttct
    60601 ggcggcgggg gttgggcttg atcaacttgc gccagatttc gccacgcccg tcattcgtga
    60661 actgctggcg ttgttgttgt gtctgttttc cggcgggtta gcgatgtatg gctacctgcg
    60721 ctggttacgc aatgaaaaag cgatgcgtct gaaagaggac ctgccctaca ccaacagctt
    60781 attaatcatt agcttaatat tgatggtcgt ggcggtgatc gttatgggac tggtgttgta
    60841 tgccggatag ccgcaaagcc agacgcattg ccgacccagg actgcagccg gagcgcacat
    60901 cactggcgtg gtttcgtacc atgctgggct acggcgcgtt gatggcgttg gctatcaaac
    60961 acaactggca ccaggcgggc atgttattct ggatttcgat tggcattctc gccatcgtgg
    61021 cgctgatcct ctggcactac acccgtaatc gcaatttaat ggatgtcacg aatagcgatt
    61081 tttcccaatt tcacgtagtc cgtgacaaat ttttgatctc cctcgcggtg ttatctctcg
    61141 caatactgtt tgctgtaacg catatacatc aacttatcgt atttattgag agagtcgcat
    61201 gacaggaagt caggtcattg atgctgaaga ggacaggcat aaactggttg tagagtacaa
    61261 ggatgctctg caacctgccg atttttatca caatttcaaa cagcggggca tccgcgccgt
    61321 acagctcatt ccctatcttg aatttgatga ccggggcgat ctgacggccg cttcggtgac
    61381 agcagagctg tgggggaaat ttttaattgc cctgttcgag tgttgggtgc gggcagatat
    61441 cagtcgtatc tcgattgagc tttttgacgc caccttacaa aaatggtgtg gcagcgaaaa
    61501 cccgcaaccg cgccgtgact gccaggcgtg cgactggcat cgcctgtgcc cgcacgctcg
    61561 ccaggagacg ccagacagcg tgctttgtgc gggctatcag gcgttttatt cttactccgc
    61621 accgcatatg cgggtgatgc gtgatctgat aaagcagcat cgttctccca tggagctgat
    61681 gacgatgctg cggtgacgtg cgcttaaacg ggctgcccct gatccgacat ccgcgtcgcc
    61741 agcggcagcc agcacagcac gatcaacaat cccattaagg tcatcaacaa ccccaggctg
    61801 ccctgaccgg tttgcggcaa catcgcagag agcgacgcca gcacgccgga accaatgttt
    61861 tgcagaccgc cgaccagcgc gccagccgtg cccgccagga aggggaacgg ctccatcgcg
    61921 ccgctggtcg ccagcggaaa cagcatcccg gcaccgaaaa agaacagcgc ggcgggaacg
    61981 agcagcgtcc agacattcat cacgccaaac cagtcgggga tccacatcag caagccagcc
    62041 agcaggcagc agataaccga ctgccacatt aacgtggaga agcgtttatt gggacgtccg
    62101 gcaaaccatg cgccaaaaaa cgctgccgga atcggcagaa taaacaaaat actgaccgtc
    62161 atactgctca gccctaacac cgcgcccatc agcacgccgg agcaggcttc aaaggcggca
    62221 atcccggcca gaccgccaat cagcatcagc aaataacagt taaaaccgct gttaccgaaa
    62281 agcgttttat aactggtaag caggcgcgtg cgcggtgcat cgaccggacg cgtttccggc
    62341 atccagcggg ccatactgaa ggtcacacca gcacaaagaa ccaacaaaaa gagataacag
    62401 gcgcgccagt tccacatcgt atccagcaga ccgccgatta gcggtgcgag caacggactg
    62461 acgagaatcc ccatgtttaa caggctgtta gcatggcgca actgtgtccg ttcatataaa
    62521 tctcgcggca aagtacgcgc cattacgccg ccaacgccgg tgcccatccc ctgcatcgcg
    62581 ctggcggcaa tcaacaccgt caaactggag gtcgtgaccg cgaccagcgt tgccagcata
    62641 aaaatggaca ttccgacgag gatcaccggt cggcggccca cgcggtcgga aatcgggcca
    62701 taaaacagct gtgagacacc gtaagtcagc agataagcgc ccattacgct ctgcaccgcc
    62761 ccttcacgga cgttgagatc gcgcgccata tcggcaatag ctggaatata aatggtttgc
    62821 gccatctgac cgacggccac gagtaatacc aacatcaata acaaattgac gtttctttgc
    62881 cttttcatta tcacggatgc ttttataaaa ataattagaa taagtcacta cgcattccca
    62941 taaatgcggg agaggaatct atcacattga tgaatgttag ccagatatac gccaggaatg
    63001 gggaattgtt tagcggcaga atatgtaaac aaaagcggca ataaatgttg ccgggaggga
    63061 ttacgtgcga actgtaaccc tctccccaaa ggggcgaggg ggaaaccacc gttccctttc
    63121 agaaaatgag ctaaacattg cacgggacgg tcccctcgcc cctttgggga gagggctagg
    63181 gtgaggggca caggcagcgc aggtgcaaac attctgctgg tagaagaagc ccccaactcc
    63241 aacccgcacg ctaaatataa aaggggagcg gttacccgct cccctttggt gcgacttgaa
    63301 tctgaattac ttcaggtatt tcagaacagc atcaagcagt tgcagtgctg caacaatgag
    63361 tttgaggata agaatggcga tatccaccag gctcatacgc gtctcctgtg gttcaggagc
    63421 actgccagct gacgtacctt tccgctgcct gttgccagct gttgtgcacg cggtatctta
    63481 aggagaaacg ttgtgcttgt tgacataaca cagtgtgctc acgcggggtt aaggcgctga
    63541 cggcaccacc cgtttcagcc aggacttagt gcgccgggta acgatgcggc ccccgcataa
    63601 cacattgcgt acagtgatat tatagaagtt tactgtataa ataaacagta atatttggac
    63661 aaaacgcaaa ctgtgatcga tactcaacta tgccctaaat acgtcaaaat tcgtgccgaa
    63721 attgcgcgtt ctgcgcggaa cacgtatact ttcagtgttg acataataca gtgtgctttg
    63781 cggttaccag ccgcaggcgg ctgatgaaac ctcgctccgg cggggttttt tgttacctgc
    63841 aattcagtac aaaacgtgat cagcccctca attttccctt gctgaaaaat tttccattgt
    63901 ctcccctgta aagctgtgct tgtataaata ttgttaaaca caaaaccaac aaggtcccca
    63961 atgactactt ccatgttcaa cgcaaaacta ctaccaactg cgctatccgc cgcagtggtc
    64021 gtcgtgcgtg tggtggtggt cgtcggcaat gcgccgtagg gactggaaca acacacgatt
    64081 ccaaaacccc gccggcgcaa accgggcggg gtttttcgtt taagcacctc ccggaaagtc
    64141 ggcccagaag aaaaggactg gagcatggca agttcgggca caacatcgac gcgtaagcgc
    64201 tttaccggcg cagaatttat cgttcatttc ctggaacagc agggcattaa gattgtgacg
    64261 ggcattccgg gcggttctat cctgcctgtt tacgatgcct taagccaaag cacgcaaatc
    64321 cgccatattc tggcccgtca tgaacagggc gcgggattta tcgctcaggg gatggcgcgc
    64381 accgacggta aaccggcggt ctgtatggcc tgtagcggac cgggtgcgac taacctggtg
    64441 accgccattg ccgatgcgcg gctggactcc atcccgctga tttgcatcac cggtcaggtt
    64501 cccgcctcga tgatcggcac cgacgccttc caggaagtgg acacctacgg catctctatc
    64561 cccatcacca aacacaacta tctggtcaga catatcgaag aactcccgca ggtcatgagc
    64621 gatgccttcc gcattgcgca atcaggccgc ccaggcccgg tgtggataga cattcctaag
    64681 gatgtgcaaa cggcagtttt tgagattgaa acacagcccg ctatggcaga aaaagccgcc
    64741 gcccccgcct ttagcgaaga aagcattcgt gacgcagcgg cgatgattaa cgctgccaaa
    64801 cgcccggtgc tttatctggg cggcggtgtg atcaatgcgc ccgcacgggt gcgtgaactg
    64861 gcggagaaag cgcaactgcc taccaccatg actttaatgg cgctgggcat gttgccaaaa
    64921 gcgcatccgt tgtcgctggg tatgctgggg atgcacggcg tgcgcagcac caactatatt
    64981 ttgcaggagg cggatttgtt gatagtgctc ggtgcgcgtt ttgatgaccg ggcgattggc
    65041 aaaaccgagc agttctgtcc gaatgccaaa attattcatg tcgatatcga tcgcgcagag
    65101 ctgggtaaaa tcaagcagcc gcatgtggcg attcaggcgg atgttgatga cgtgctggcg
    65161 caattgatcc cgcaggtgga agcgcaaccg cgtgcagagt ggcaccagtt ggtagcggat
    65221 ttgcagcgcg aattcccgtg tccaatcccg aaagcgtgcg atccattaag ccattacggc
    65281 ctgatcaacg ccgttgccgc ctgtgtcgat gacaatgcga ttatcaccac cgatgtgggt
    65341 cagcatcaga tgtggaccgc gcaagcttat ccgctcaatc gcccacgcca gtggctgacc
    65401 tccggtgggc tgggcacgat gggttttggc ctgcctgcgg cgattggcgc ggcgctggcg
    65461 aacccggatc gcaaagtgtt gtgtttctcc ggcgacggca gcctgatgat gaatattcag
    65521 gagatggcga ccgccagtga aaatcagctg gatgtcaaaa tcattctgat gaacaacgaa
    65581 gcgctggggc tggtgcatca gcaacagagt ctgttctacg agcaaggcgt ttttgccgcc
    65641 acctatccgg gaaaaatcaa ctttatgcag attgccgccg gattcggcct cgaaacctgt
    65701 gatttgaata acgaagccga tccgcaggct gcattgcagg aaatcatcaa tcgccctggc
    65761 ccggcgctga tccatgtgcg cattgatgcc gaagaaaaag tttacccgat ggtgccgcca
    65821 ggtgcggcga atactgaaat ggtgggggaa taagccatgc aaaacacaac tcatgacaac
    65881 gtgattctgg agctcaccgt tcgcaaccat ccgggcgtaa tgacccacgt ttgtggcctt
    65941 tttgcccgcc gcgcttttaa cgttgaaggc attctttgtc tgccgattca ggacagcgac
    66001 aaaagccata tctggctact ggtcaatgac gaccagcgtc tggagcagat gataagccaa
    66061 atcgataagc tggaagatgt ggtgaaagtg cagcgtaatc agtccgatcc gactatgttt
    66121 aacaagattg cggtgttttt tcagtaaccg ctcaaggctt gaacaacatc gcgcttatcg
    66181 ttaaggtaag cgcgtttttt tttatccgcc aggacaagac catgatcacc gttgccctta
    66241 tagacgatca cctcatcgtc cgctccggct ttgcgcagct gctggggctg gaacctgatt
    66301 tgcaggtagt tgccgagttt ggttcggggc gcgaggcgct ggcggggctg ccggggcgcg
    66361 gtgtgcaggt gtgtatttgc gatatctcca tgcccgatat ctccggtctg gaactgctaa
    66421 gccagctgcc gaaaggtatg gcgacaataa tgctctctgt tcatgacagt ccggcgctgg
    66481 ttgagcaggc gcttaacgcg ggggcgcgcg gctttctctc caaacgctgt agccctgacg
    66541 aactgattgc ggcggtgcat acggttgcca cgggcggctg ttatctgacg ccggatattg
    66601 ccattaaact ggcatccggt cgtcaggacc cgctgaccaa acgtgaacgc caggtggcgg
    66661 aaaaactggc gcaaggaatg gcggtgaaag agattgccgc cgaactgggc ttgtcaccga
    66721 aaacggtaca cgtccatcgc gccaatctga tggaaaaact gggcgtcagt aacgacgtag
    66781 aactggcgcg ccgcatgttt gatggctggt gatgaagacg ttgttctccc gcttaattac
    66841 cgttattgcc tgctttttta tcttctctgc cgcatggttt tgcctgtgga gtatcagcct
    66901 gcacctggtt gagcgccctg atatggcggt gctgttattt ccgtttggtc tgcggctagg
    66961 gctaatgctg caatgcccgc gcggatactg gcccgtattg ctgggtgcgg aatggctgct
    67021 gatttactgg ctaacgcagg cggtcggttt aacccatttt ccgttattga tgatcggtag
    67081 tttactgacg ttactgcccg tagcgctgat ctcgcgctat cgccatcagc gtgactggcg
    67141 caccttgctg ttacaggggg cggcgttaac ggcggcggcg ttgttgcagt cgctgccctg
    67201 gctttggcac ggcaaagagt cgtggaatgc gctgttgctg actttaactg gcggcctgac
    67261 gctggccccg atatgtctgg tgttctggca ctatctcgcc aataacacct ggctgccgct
    67321 cggcccgtca ctggtttctc aaccgatcaa ctggcgcggg cggcatctgg tctggtactt
    67381 gctgttgttt gttattagtc tctggctcca gttgggattg ccggacgaac tgtcgcgctt
    67441 tacgccattc tgcctggcgc tgccgattat cgcgctggcc tggcactacg gttggcaagg
    67501 ggcgctgatc gcgacgttga tgaacgccat cgcgctgatc gccagtcaaa cctggcgcga
    67561 tcatccggtg gatttattgc tctcgttgct ggtgcaaagt ctgacagggt tgttactggg
    67621 cgctggcatc cagcggttgc gtgaacttaa ccagtcgctg caaaaggaac tggcgcgcaa
    67681 tcagcatctg gctgaacggt tgctggaaac cgaagagagc gtgcgtcgtg atgtggcgcg
    67741 tgagctgcat gatgatatcg gtcagaccat cactgctatt cgtactcagg cgggcattgt
    67801 tcagcggctg gcggcagata acgccagcgt gaagcagagc gggcagctca tcgaacaact
    67861 atcgctgggc gtttacgacg cggtgcgccg tttgttgggt cggttacgtc cgcgccagtt
    67921 ggatgatctc accctggagc aggccatccg ctcactgatg cgggaaatgg agctggaagg
    67981 gcgcggtatt gtcagccatc tcgaatggcg aatcgatgaa tcagcattaa gtgaaaacca
    68041 gcgcgtgacg ctgtttcgtg tctgccagga agggctgaac aacattgtga aacatgctga
    68101 tgccagcgcg gtcacgctgc aaggctggca gcaggatgaa cggttgatgc tggttattga
    68161 agacgatggc agcggtttgc cgccgggttc cgggcaacaa ggttttggcc tcaccggaat
    68221 gcgcgagcgc gtaacggcgc tgggtggcac attacacatt tcctgtctgc acggcacgcg
    68281 tgtcagcgtt tctctacctc aacgctatgt ctaaggtttg atgatgttgc cgtttctgaa
    68341 agcgcctgcc gatgcgccat taatgactga taaatatgaa attgatgccc gctatcgcta
    68401 ctggcgtcgg catattctgc tgaccatctg gctgggttac gcgctgtttt acttcacgcg
    68461 taaaagtttt aacgccgccg taccagaaat ccttgctaac ggcgtgctca gccgtagcga
    68521 tatcggcctg ttagcgaccc tgttttacat tacctacggc gtgtcgaagt ttgtctccgg
    68581 cattgtcagc gatcgctcaa atgcccgtta ttttatgggg atagggctta tcgccacggg
    68641 catcatcaac attctgtttg gcttctcgac gtcgctatgg gcgtttgccg tgctctgggt
    68701 gctgaacgcc tttttccagg gctggggttc accggtgtgt gcgcgtctgt taacggcctg
    68761 gtattcacgt accgagcgcg gcggttggtg ggcattatgg aacacggcgc ataacgtcgg
    68821 cggcgcactc attcccattg tgatggcagc gagcgcgctg cattacggct ggcgtgccgg
    68881 gatgatgatt gctggttgta tggcgatagt cgtggggatt tttctctgct ggcggctacg
    68941 cgatcgcccg caggcgttag gtttaccggc ggtcggtgaa tggcggcacg acgcgctgga
    69001 aattgctcaa caacaagaag gggcagggtt gacgcgtaaa gagatcctga ccaaatatgt
    69061 gttgctgaat ccgtatatct ggctgctttc gttttgctat gtgctggtct atgtggtccg
    69121 ggcggcgatc aacgactggg gcaatttgta tatgtccgag acactgggcg tcgatctggt
    69181 cacggcgaat acggcagtga cgatgtttga actgggcgga tttatcggtg cgctggtagc
    69241 cggttggggc tcggacaaat tgtttaacgg caaccgtggg ccgatgaatt tgattttcgc
    69301 cgccggaatt ttgctttcag tcggctcgct gtggctgatg ccatttgcca gctacgtgat
    69361 gcaggcaacc tgcttcttca ccattggttt ttttgtcttt ggcccgcaga tgttaatcgg
    69421 catggcggcg gcagagtgtt cccacaaaga ggcggcaggg gcggcgacgg ggtttgtcgg
    69481 cttgtttgct tatctggggg cgtcgcttgc tggttggccg ctggcgaaag tactcgatac
    69541 ctggcactgg agcggatttt ttgtggttat ctctatcgcc gccgggattt ccgcactgct
    69601 gttactgccc tttttgaacg cccaggcacc gcgcgaagcg tgatgcatct caccttttca
    69661 cttcatatcc ggcaaaacta agaaattttc caggttttgc ctggacgcta tctcaggcct
    69721 gatttgctgc tgatttttac aatgcatgcc tcacgcaggt attcatttca ggagtaaccc
    69781 atgctggctt tcttaaacca ggttcgcaag ccgaccctgg accttccgct cgaagtgcgg
    69841 cgcaaaatgt ggttcaaacc gttcatgcaa tcctacctgg tggtctttat cggctatctg
    69901 acgatgtacc tgattcgcaa gaactttaac atcgcgcaga acgacatgat ctcgacctac
    69961 gggttgagca tgacgcagct ggggatgatc ggcctgggtt tctccatcac ttatggcgtg
    70021 ggtaaaacgc tggtttccta ctacgccgac ggcaaaaaca ccaaacaatt cctgccgttc
    70081 atgctgatcc tgtctgccat ttgtatgctg ggcttcagtg ccagtatggg cagcggctcg
    70141 gttagcctgt tcctgatgat tgccttctac gccttaagcg gctttttcca gagtaccggc
    70201 ggttcgtgca gttactccac catcaccaaa tggacaccgc gtcgtaagcg cgggaccttc
    70261 ctcggtttct ggaacatttc ccataacctt ggcggcgcgg gtgcggctgg tgtggcgctg
    70321 ttcggcgcaa attacctgtt cgacggccac gtgatcggca tgtttatctt cccgtcgatt
    70381 atcgcgttga ttgtcggttt tatcggcctg cgttacggca gcgactcccc ggaatcttat
    70441 ggcctcggca aagcggaaga gctgttcggc gaggagatca gcgaagagga caaagagaca
    70501 gaatctaccg atatgaccaa gtggcagatc tttgttgagt atgtgctgaa aaacaaagtg
    70561 atctggctgc tgtgcttcgc caacattttc ctctatgtgg tgcgtattgg tatcgaccag
    70621 tggtcaaccg tgtacgcgtt ccaggaactg aaactctcta aagcggtggc gattcagggc
    70681 tttacgctgt ttgaagctgg tgcgctggtc ggtacgctgc tgtggggctg gctctctgac
    70741 ctggcgaacg gtcgccgtgg cctggtggcc tgcatcgcgc tggcgctgat tatcgccacg
    70801 ctcggtgtgt atcaacatgc cagcaacgaa tatatctatc tggcttctct ctttgcgttg
    70861 ggtttcctgg tctttggccc gcaattgttg attggtgtgg ctgctgttgg ctttgtacct
    70921 aaaaaagcga ttggcgctgc cgatggtatt aaaggcacct ttgcttacct gattggtgac
    70981 agctttgcca agttaggtct gggaatgatt gccgatggga cgccggtatt cggccttacc
    71041 ggctgggcag gcaccttcgc cgcacttgat atcgccgcga ttggttgtat ctgcctgatg
    71101 gcgatagtgg cggtgatgga agaacgcaaa atccgccgcg agaaaaaaat tcagcagttg
    71161 acagtggcat aaacgtaact ggtgactttt gcccggcatg acgccgggct tttttattat
    71221 tccgtgactt ccagcgtagt gaaggcaaac ttctcgccat caaatagccc ctgactggtt
    71281 agttttagcg cggggatcac tggcagagaa agaaacgcca tctgaataaa cggctcatcg
    71341 ggtaacggac cgcattcacg ggcggcggct ttcaaggcgt caatttgttc cgccagcaac
    71401 tgcgccgtgt cggtgctcat caggccggca atgggcaacg gcagatgact ttggacctgg
    71461 ccgttacgta ccacgcacag cccgccgcca tcctgaatca cctgattgac cgccagcgcc
    71521 atctcttcgg cactgcgacc gatcaccaca atattatggc tgtcatggct gaccgtcgca
    71581 gccagcgcac cttcattcag tccaaagccg ccaagcaaac cacaagccgg agccagccgt
    71641 tgcccgtaac gctcaagtac ggcaataaag ctcacatcat cgcgatcaaa accattttcg
    71701 ctgtagacgc tggagtggga gtgcgtaatc aattcgttat ggatgacgtc aatgacccga
    71761 tagcgttttc cgggcgtaaa ttgcagggca aagtcgctgg cggaaactgg ctggcgggca
    71821 atggtgttgc catatggcgg agcggattgt gccagtctcg ccgactcttc cgcctgtaag
    71881 gtttgcgcat caatcggctc gcctttcacc agtacctgct gcaccgtgac cttacgcgca
    71941 tcgctcaaca ggacgatatc ggcctgcttg ccgggtgcca gtaagccgag gtgattcaga
    72001 ccaaagtggc gcgccgtcga ccagctggcg acgcgatatg ccacatgcag cggcacattg
    72061 tgttgttcga tcaggcggcg aattaaggca tcgatgtgtc cttcatgggc gatctcccac
    72121 gggttacggt catcggtaca gagcatgcat tgcgggctgt taaattcgtt gatcaacggt
    72181 gccagcgcgt tgagattgcg ggcagcggac ccttcgcgga tcatcaacga catgccgagt
    72241 tgtaatttcc ggcgtccttc ttccagctga taactttcgt ggcagttttc aatacccgca
    72301 gtaatatagg cgttaagttc tttaccaccc aaacccgggc agtgaccgtc cagcgtcagg
    72361 tggcgaaatg catccagttt atcgagcagc gcattctgcc cgctaattac gccagggtag
    72421 tccatcattt ctgcaaggcc ggtaacctgc ggatggtccc gccaggcgag catctgttca
    72481 agggtaaaac tggcaccgtt aacatcgcag ccttccaggg cgggtacgca agagctgacc
    72541 tgtaagtact ggttttgcct tgcctgttcg gcacagcggg caaaccaggc gaatccggct
    72601 tctcccatca cgttgacgat ttcatgaggg tcgcaaataa cggtcgtcag gccgcgcggc
    72661 agggtagcgg tttcaaaagt gaccggcgtc atcatgctgg attcaatatg caggtgagca
    72721 tcaataaacc ctggcaccgc cgttgcgccg cgagcatcaa tccgctgcaa agccggagca
    72781 tcagcgtatt ctgcgccaac accggcaatg taacgtcctt taatcacaat tgggccggaa
    72841 atttctccgc cattgatcag gtcgagaata gagacattat caataatata atcggcaaca
    72901 gcgtcgccac gggaaacggc taacaattcc tggtattcag cccgg
//
LOCUS       DAZGAN020000011        72640 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGAN020000011 DAZGAN020000000
VERSION     DAZGAN020000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 72640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.011"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..605)
                     /gene="rfbA"
                     /locus_tag="ACHKYO_RS04220"
                     /old_locus_tag="pgaptmp_000844"
     CDS             complement(<1..605)
                     /gene="rfbA"
                     /locus_tag="ACHKYO_RS04220"
                     /old_locus_tag="pgaptmp_000844"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGU4427737.1"
                     /translation="MKGIILAGGSGTRLYPITMGISKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILIITTPEDQFSFIRLLGDGKHLGVSISYAVQPRPDGLAQAFTIGKDFIGADSV
                     CLVLGDNIFFGQGFTPKLKTACERKLGATIFGYQVRDPGRFGVVEFDSDYRVLSIEEK
                     PLKPKSNWAVTGLYFYDNDVVEIAKSITPSQRGELEITSIN"
     gene            complement(602..1678)
                     /gene="rfbB"
                     /locus_tag="ACHKYO_RS04225"
                     /old_locus_tag="pgaptmp_000845"
     CDS             complement(602..1678)
                     /gene="rfbB"
                     /locus_tag="ACHKYO_RS04225"
                     /old_locus_tag="pgaptmp_000845"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGU4427738.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTINWY
                     LNNNDWCRRVQDGSYQRERLGVIR"
     gene            complement(2051..2944)
                     /gene="galF"
                     /locus_tag="ACHKYO_RS04230"
                     /old_locus_tag="pgaptmp_000846"
     CDS             complement(2051..2944)
                     /gene="galF"
                     /locus_tag="ACHKYO_RS04230"
                     /old_locus_tag="pgaptmp_000846"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HGU4427739.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGANFRKGIEKLLSE"
     gene            complement(3119..4513)
                     /gene="wcaM"
                     /locus_tag="ACHKYO_RS04235"
                     /old_locus_tag="pgaptmp_000847"
     CDS             complement(3119..4513)
                     /gene="wcaM"
                     /locus_tag="ACHKYO_RS04235"
                     /old_locus_tag="pgaptmp_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HGU4427740.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPDQQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEKGGSMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNALSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(4524..5744)
                     /gene="wcaL"
                     /locus_tag="ACHKYO_RS04240"
                     /old_locus_tag="pgaptmp_000848"
     CDS             complement(4524..5744)
                     /gene="wcaL"
                     /locus_tag="ACHKYO_RS04240"
                     /old_locus_tag="pgaptmp_000848"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HGU4427741.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPAGKVAKLRHRASQTLRGIYRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSRDV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(5741..7021)
                     /gene="wcaK"
                     /locus_tag="ACHKYO_RS04245"
                     /old_locus_tag="pgaptmp_000849"
     CDS             complement(5741..7021)
                     /gene="wcaK"
                     /locus_tag="ACHKYO_RS04245"
                     /old_locus_tag="pgaptmp_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HGU4427742.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(7200..8678)
                     /gene="wzxC"
                     /locus_tag="ACHKYO_RS04250"
                     /old_locus_tag="pgaptmp_000850"
     CDS             complement(7200..8678)
                     /gene="wzxC"
                     /locus_tag="ACHKYO_RS04250"
                     /old_locus_tag="pgaptmp_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HGU4427743.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWISIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(8680..10074)
                     /gene="wcaJ"
                     /locus_tag="ACHKYO_RS04255"
                     /old_locus_tag="pgaptmp_000851"
     CDS             complement(8680..10074)
                     /gene="wcaJ"
                     /locus_tag="ACHKYO_RS04255"
                     /old_locus_tag="pgaptmp_000851"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HGU4427744.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(10129..11499)
                     /gene="cpsG"
                     /locus_tag="ACHKYO_RS04260"
                     /old_locus_tag="pgaptmp_000852"
                     /gene_synonym="manB"
     CDS             complement(10129..11499)
                     /gene="cpsG"
                     /locus_tag="ACHKYO_RS04260"
                     /old_locus_tag="pgaptmp_000852"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGU4427745.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     SYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPIEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(11692..13128)
                     /gene="cpsB"
                     /locus_tag="ACHKYO_RS04265"
                     /old_locus_tag="pgaptmp_000853"
     CDS             complement(11692..13128)
                     /gene="cpsB"
                     /locus_tag="ACHKYO_RS04265"
                     /old_locus_tag="pgaptmp_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HGU4427746.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(13131..14354)
                     /gene="wcaI"
                     /locus_tag="ACHKYO_RS04270"
                     /old_locus_tag="pgaptmp_000854"
     CDS             complement(13131..14354)
                     /gene="wcaI"
                     /locus_tag="ACHKYO_RS04270"
                     /old_locus_tag="pgaptmp_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HGU4427747.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNIIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKYNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(14351..14830)
                     /gene="gmm"
                     /locus_tag="ACHKYO_RS04275"
                     /old_locus_tag="pgaptmp_000855"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(14351..14830)
                     /gene="gmm"
                     /locus_tag="ACHKYO_RS04275"
                     /old_locus_tag="pgaptmp_000855"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HGU4427748.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(14830..15798)
                     /gene="fcl"
                     /locus_tag="ACHKYO_RS04280"
                     /old_locus_tag="pgaptmp_000856"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(14830..15798)
                     /gene="fcl"
                     /locus_tag="ACHKYO_RS04280"
                     /old_locus_tag="pgaptmp_000856"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HGU4427749.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(15801..16922)
                     /gene="gmd"
                     /locus_tag="ACHKYO_RS04285"
                     /old_locus_tag="pgaptmp_000857"
     CDS             complement(15801..16922)
                     /gene="gmd"
                     /locus_tag="ACHKYO_RS04285"
                     /old_locus_tag="pgaptmp_000857"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGU4427750.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(16948..17496)
                     /gene="wcaF"
                     /locus_tag="ACHKYO_RS04290"
                     /old_locus_tag="pgaptmp_000858"
     CDS             complement(16948..17496)
                     /gene="wcaF"
                     /locus_tag="ACHKYO_RS04290"
                     /old_locus_tag="pgaptmp_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HGU4427751.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(17512..18258)
                     /gene="wcaE"
                     /locus_tag="ACHKYO_RS04295"
                     /old_locus_tag="pgaptmp_000859"
     CDS             complement(17512..18258)
                     /gene="wcaE"
                     /locus_tag="ACHKYO_RS04295"
                     /old_locus_tag="pgaptmp_000859"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HGU4427752.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQNAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(18269..19486)
                     /gene="wcaD"
                     /locus_tag="ACHKYO_RS04300"
                     /old_locus_tag="pgaptmp_000860"
     CDS             complement(18269..19486)
                     /gene="wcaD"
                     /locus_tag="ACHKYO_RS04300"
                     /old_locus_tag="pgaptmp_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HGU4427753.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(19461..20678)
                     /gene="wcaC"
                     /locus_tag="ACHKYO_RS04305"
                     /old_locus_tag="pgaptmp_000861"
     CDS             complement(19461..20678)
                     /gene="wcaC"
                     /locus_tag="ACHKYO_RS04305"
                     /old_locus_tag="pgaptmp_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HGU4427754.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIVLFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(20675..21163)
                     /gene="wcaB"
                     /locus_tag="ACHKYO_RS04310"
                     /old_locus_tag="pgaptmp_000862"
     CDS             complement(20675..21163)
                     /gene="wcaB"
                     /locus_tag="ACHKYO_RS04310"
                     /old_locus_tag="pgaptmp_000862"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HGU4427755.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNIWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(21166..22005)
                     /gene="wcaA"
                     /locus_tag="ACHKYO_RS04315"
                     /old_locus_tag="pgaptmp_000863"
     CDS             complement(21166..22005)
                     /gene="wcaA"
                     /locus_tag="ACHKYO_RS04315"
                     /old_locus_tag="pgaptmp_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HGU4427756.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(22183..24345)
                     /gene="wzc"
                     /locus_tag="ACHKYO_RS04320"
                     /old_locus_tag="pgaptmp_000864"
     CDS             complement(22183..24345)
                     /gene="wzc"
                     /locus_tag="ACHKYO_RS04320"
                     /old_locus_tag="pgaptmp_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HGU4427757.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(24348..24791)
                     /gene="wzb"
                     /locus_tag="ACHKYO_RS04325"
                     /old_locus_tag="pgaptmp_000865"
     CDS             complement(24348..24791)
                     /gene="wzb"
                     /locus_tag="ACHKYO_RS04325"
                     /old_locus_tag="pgaptmp_000865"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HGU4427758.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(24797..25936)
                     /gene="wza"
                     /locus_tag="ACHKYO_RS04330"
                     /old_locus_tag="pgaptmp_000866"
     CDS             complement(24797..25936)
                     /gene="wza"
                     /locus_tag="ACHKYO_RS04330"
                     /old_locus_tag="pgaptmp_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HGU4427759.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            26245..26394
                     /locus_tag="ACHKYO_RS04335"
                     /old_locus_tag="pgaptmp_000867"
     CDS             26245..26394
                     /locus_tag="ACHKYO_RS04335"
                     /old_locus_tag="pgaptmp_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4427760.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            26595..28178
                     /gene="yegH"
                     /locus_tag="ACHKYO_RS04340"
                     /old_locus_tag="pgaptmp_000868"
     CDS             26595..28178
                     /gene="yegH"
                     /locus_tag="ACHKYO_RS04340"
                     /old_locus_tag="pgaptmp_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU4427761.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(28452..30305)
                     /gene="asmA"
                     /locus_tag="ACHKYO_RS04345"
                     /old_locus_tag="pgaptmp_000869"
     CDS             complement(28452..30305)
                     /gene="asmA"
                     /locus_tag="ACHKYO_RS04345"
                     /old_locus_tag="pgaptmp_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HGU4427762.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILNAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(30327..30908)
                     /gene="dcd"
                     /locus_tag="ACHKYO_RS04350"
                     /old_locus_tag="pgaptmp_000870"
     CDS             complement(30327..30908)
                     /gene="dcd"
                     /locus_tag="ACHKYO_RS04350"
                     /old_locus_tag="pgaptmp_000870"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HGU4427763.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(31000..31641)
                     /gene="udk"
                     /locus_tag="ACHKYO_RS04355"
                     /old_locus_tag="pgaptmp_000871"
     CDS             complement(31000..31641)
                     /gene="udk"
                     /locus_tag="ACHKYO_RS04355"
                     /old_locus_tag="pgaptmp_000871"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGU4427764.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            31959..35277
                     /gene="dgcE"
                     /locus_tag="ACHKYO_RS04360"
                     /old_locus_tag="pgaptmp_000872"
                     /pseudo
     CDS             31959..35277
                     /gene="dgcE"
                     /locus_tag="ACHKYO_RS04360"
                     /old_locus_tag="pgaptmp_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(35316..36173)
                     /gene="alkA"
                     /locus_tag="ACHKYO_RS04365"
                     /old_locus_tag="pgaptmp_000873"
     CDS             complement(35316..36173)
                     /gene="alkA"
                     /locus_tag="ACHKYO_RS04365"
                     /old_locus_tag="pgaptmp_000873"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HGU4427765.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDMARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            36307..37659
                     /gene="yegD"
                     /locus_tag="ACHKYO_RS04370"
                     /old_locus_tag="pgaptmp_000874"
     CDS             36307..37659
                     /gene="yegD"
                     /locus_tag="ACHKYO_RS04370"
                     /old_locus_tag="pgaptmp_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4427766.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(37672..39612)
                     /gene="yegI"
                     /locus_tag="ACHKYO_RS04375"
                     /old_locus_tag="pgaptmp_000875"
     CDS             complement(37672..39612)
                     /gene="yegI"
                     /locus_tag="ACHKYO_RS04375"
                     /old_locus_tag="pgaptmp_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HGU4427767.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEQLRQAAEKLAQAQADLSRC"
     gene            complement(39609..40370)
                     /gene="pphC"
                     /locus_tag="ACHKYO_RS04380"
                     /old_locus_tag="pgaptmp_000876"
     CDS             complement(39609..40370)
                     /gene="pphC"
                     /locus_tag="ACHKYO_RS04380"
                     /old_locus_tag="pgaptmp_000876"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HGU4427768.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(40367..41026)
                     /gene="yegL"
                     /locus_tag="ACHKYO_RS04385"
                     /old_locus_tag="pgaptmp_000877"
     CDS             complement(40367..41026)
                     /gene="yegL"
                     /locus_tag="ACHKYO_RS04385"
                     /old_locus_tag="pgaptmp_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU4427769.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIA
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLHSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(41247..41306)
                     /gene="ibsA"
                     /locus_tag="ACHKYO_RS04390"
                     /old_locus_tag="pgaptmp_000878"
     CDS             complement(41247..41306)
                     /gene="ibsA"
                     /locus_tag="ACHKYO_RS04390"
                     /old_locus_tag="pgaptmp_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HGU4427770.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(41579..41635)
                     /locus_tag="ACHKYO_RS04395"
                     /old_locus_tag="pgaptmp_000879"
     CDS             complement(41579..41635)
                     /locus_tag="ACHKYO_RS04395"
                     /old_locus_tag="pgaptmp_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4427771.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            41914..43161
                     /gene="mdtA"
                     /locus_tag="ACHKYO_RS04400"
                     /old_locus_tag="pgaptmp_000880"
     CDS             41914..43161
                     /gene="mdtA"
                     /locus_tag="ACHKYO_RS04400"
                     /old_locus_tag="pgaptmp_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HGU4427772.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLIALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKVGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            43161..46283
                     /gene="mdtB"
                     /locus_tag="ACHKYO_RS04405"
                     /old_locus_tag="pgaptmp_000881"
     CDS             43161..46283
                     /gene="mdtB"
                     /locus_tag="ACHKYO_RS04405"
                     /old_locus_tag="pgaptmp_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HGU4427773.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSAHLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGVSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            46284..49361
                     /gene="mdtC"
                     /locus_tag="ACHKYO_RS04410"
                     /old_locus_tag="pgaptmp_000882"
     CDS             46284..49361
                     /gene="mdtC"
                     /locus_tag="ACHKYO_RS04410"
                     /old_locus_tag="pgaptmp_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HGU4427774.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFTST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            49362..50777
                     /gene="mdtD"
                     /locus_tag="ACHKYO_RS04415"
                     /old_locus_tag="pgaptmp_000883"
     CDS             49362..50777
                     /gene="mdtD"
                     /locus_tag="ACHKYO_RS04415"
                     /old_locus_tag="pgaptmp_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4427775.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNCFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            50774..52177
                     /gene="baeS"
                     /locus_tag="ACHKYO_RS04420"
                     /old_locus_tag="pgaptmp_000884"
     CDS             50774..52177
                     /gene="baeS"
                     /locus_tag="ACHKYO_RS04420"
                     /old_locus_tag="pgaptmp_000884"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HGU4427776.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPVPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            52174..52896
                     /gene="baeR"
                     /locus_tag="ACHKYO_RS04425"
                     /old_locus_tag="pgaptmp_000885"
     CDS             52174..52896
                     /gene="baeR"
                     /locus_tag="ACHKYO_RS04425"
                     /old_locus_tag="pgaptmp_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HGU4427777.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            53087..53419
                     /gene="yegP"
                     /locus_tag="ACHKYO_RS04430"
                     /old_locus_tag="pgaptmp_000886"
     CDS             53087..53419
                     /gene="yegP"
                     /locus_tag="ACHKYO_RS04430"
                     /old_locus_tag="pgaptmp_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HGU4427778.1"
                     /translation="MAGWFELIKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKVNGT
                     SQTVKDNT"
     gene            53628..53924
                     /locus_tag="ACHKYO_RS04435"
                     /old_locus_tag="pgaptmp_000887"
     CDS             53628..53924
                     /locus_tag="ACHKYO_RS04435"
                     /old_locus_tag="pgaptmp_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU4427779.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            53926..54222
                     /locus_tag="ACHKYO_RS04440"
                     /old_locus_tag="pgaptmp_000888"
     CDS             53926..54222
                     /locus_tag="ACHKYO_RS04440"
                     /old_locus_tag="pgaptmp_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4427780.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            54325..55686
                     /gene="yegQ"
                     /locus_tag="ACHKYO_RS04445"
                     /old_locus_tag="pgaptmp_000889"
     CDS             54325..55686
                     /gene="yegQ"
                     /locus_tag="ACHKYO_RS04445"
                     /old_locus_tag="pgaptmp_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HGU4427781.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(56016..56333)
                     /gene="yegR"
                     /locus_tag="ACHKYO_RS04450"
                     /old_locus_tag="pgaptmp_000890"
     CDS             complement(56016..56333)
                     /gene="yegR"
                     /locus_tag="ACHKYO_RS04450"
                     /old_locus_tag="pgaptmp_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HGU4427782.1"
                     /translation="MKKIAAISLISIFIVSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            56739..57638
                     /gene="yegS"
                     /locus_tag="ACHKYO_RS04455"
                     /old_locus_tag="pgaptmp_000891"
     CDS             56739..57638
                     /gene="yegS"
                     /locus_tag="ACHKYO_RS04455"
                     /old_locus_tag="pgaptmp_000891"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HGU4427783.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(57720..58493)
                     /locus_tag="ACHKYO_RS04460"
                     /old_locus_tag="pgaptmp_000892"
     CDS             complement(57720..58493)
                     /locus_tag="ACHKYO_RS04460"
                     /old_locus_tag="pgaptmp_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4427784.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(58599..59639)
                     /gene="gatD"
                     /locus_tag="ACHKYO_RS04465"
                     /old_locus_tag="pgaptmp_000893"
     CDS             complement(58599..59639)
                     /gene="gatD"
                     /locus_tag="ACHKYO_RS04465"
                     /old_locus_tag="pgaptmp_000893"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU4427785.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(59687..61042)
                     /locus_tag="ACHKYO_RS04470"
                     /old_locus_tag="pgaptmp_000894"
     CDS             complement(59687..61042)
                     /locus_tag="ACHKYO_RS04470"
                     /old_locus_tag="pgaptmp_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGU4427786.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIEVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(61046..61330)
                     /gene="gatB"
                     /locus_tag="ACHKYO_RS04475"
                     /old_locus_tag="pgaptmp_000895"
     CDS             complement(61046..61330)
                     /gene="gatB"
                     /locus_tag="ACHKYO_RS04475"
                     /old_locus_tag="pgaptmp_000895"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HGU4427787.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(61361..61813)
                     /gene="gatA"
                     /locus_tag="ACHKYO_RS04480"
                     /old_locus_tag="pgaptmp_000896"
     CDS             complement(61361..61813)
                     /gene="gatA"
                     /locus_tag="ACHKYO_RS04480"
                     /old_locus_tag="pgaptmp_000896"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HGU4427788.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(61823..63080)
                     /gene="gatZ"
                     /locus_tag="ACHKYO_RS04485"
                     /old_locus_tag="pgaptmp_000897"
                     /pseudo
     CDS             complement(61823..63080)
                     /gene="gatZ"
                     /locus_tag="ACHKYO_RS04485"
                     /old_locus_tag="pgaptmp_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            complement(63109..63963)
                     /gene="gatY"
                     /locus_tag="ACHKYO_RS04490"
                     /old_locus_tag="pgaptmp_000898"
     CDS             complement(63109..63963)
                     /gene="gatY"
                     /locus_tag="ACHKYO_RS04490"
                     /old_locus_tag="pgaptmp_000898"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HGU4427789.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(64272..65324)
                     /gene="fbaB"
                     /locus_tag="ACHKYO_RS04495"
                     /old_locus_tag="pgaptmp_000899"
     CDS             complement(64272..65324)
                     /gene="fbaB"
                     /locus_tag="ACHKYO_RS04495"
                     /old_locus_tag="pgaptmp_000899"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HGU4427790.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYLGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            65581..66858
                     /gene="yegT"
                     /locus_tag="ACHKYO_RS04500"
                     /old_locus_tag="pgaptmp_000900"
     CDS             65581..66858
                     /gene="yegT"
                     /locus_tag="ACHKYO_RS04500"
                     /old_locus_tag="pgaptmp_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HGU4427791.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            66855..67859
                     /gene="yegU"
                     /locus_tag="ACHKYO_RS04505"
                     /old_locus_tag="pgaptmp_000901"
     CDS             66855..67859
                     /gene="yegU"
                     /locus_tag="ACHKYO_RS04505"
                     /old_locus_tag="pgaptmp_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGU4427792.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            67856..68821
                     /gene="yegV"
                     /locus_tag="ACHKYO_RS04510"
                     /old_locus_tag="pgaptmp_000902"
     CDS             67856..68821
                     /gene="yegV"
                     /locus_tag="ACHKYO_RS04510"
                     /old_locus_tag="pgaptmp_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGU4427793.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(68795..69541)
                     /gene="yegW"
                     /locus_tag="ACHKYO_RS04515"
                     /old_locus_tag="pgaptmp_000903"
     CDS             complement(68795..69541)
                     /gene="yegW"
                     /locus_tag="ACHKYO_RS04515"
                     /old_locus_tag="pgaptmp_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4427794.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(69593..70411)
                     /gene="yegX"
                     /locus_tag="ACHKYO_RS04520"
                     /old_locus_tag="pgaptmp_000904"
     CDS             complement(69593..70411)
                     /gene="yegX"
                     /locus_tag="ACHKYO_RS04520"
                     /old_locus_tag="pgaptmp_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGU4427795.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFLQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(70476..71276)
                     /gene="thiD"
                     /locus_tag="ACHKYO_RS04525"
                     /old_locus_tag="pgaptmp_000905"
     CDS             complement(70476..71276)
                     /gene="thiD"
                     /locus_tag="ACHKYO_RS04525"
                     /old_locus_tag="pgaptmp_000905"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGU4427796.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(71273..72061)
                     /gene="thiM"
                     /locus_tag="ACHKYO_RS04530"
                     /old_locus_tag="pgaptmp_000906"
     CDS             complement(71273..72061)
                     /gene="thiM"
                     /locus_tag="ACHKYO_RS04530"
                     /old_locus_tag="pgaptmp_000906"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGU4427797.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHEFLSFKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(72096..72192)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(72284..72556)
                     /gene="rcnR"
                     /locus_tag="ACHKYO_RS04535"
                     /old_locus_tag="pgaptmp_000907"
     CDS             complement(72284..72556)
                     /gene="rcnR"
                     /locus_tag="ACHKYO_RS04535"
                     /old_locus_tag="pgaptmp_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HGU4427798.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
BASE COUNT        17208 a        19279 c        18303 g        17850 t
ORIGIN      
        1 tcatttatcg aagttatttc tagctcacct cgttgagaag gagtaattga tttggcaatt
       61 tcaacaacat cattatcata aaaataaagt ccagttacag cccagtttga ttttggtttt
      121 aacggttttt cttcaatact taatacccta taatctgagt caaactctac aactccaaac
      181 cgtcctggat ctcttacctg atagccaaaa attgtggctc ctaatttcct ttcacatgca
      241 gtttttaatt ttggtgtaaa cccttgacca aaaaaaatat tatcacctaa tacaaggcaa
      301 actgaatcag cacctataaa atcttttcca attgtgaaag cttgcgctaa accatctggc
      361 cttggttgca ctgcataact aatgctgaca cctagatgtt ttccgtcacc aagcaaccgt
      421 ataaaactaa attggtcttc gggcgttgta ataataagta tatctctgat ccctgccaac
      481 atcagaacag acaatggata atatatcatt ggcttgtcat aaacaggcaa taattgcttt
      541 gaaatcccca ttgtaattgg ataaagcctt gttccagaac caccagctaa aataattcct
      601 ttcatctaat tacccccaaa cgttcacgtt gataagagcc atcttgaact cgccggcacc
      661 aatcattatt attcaaatac cagttaattg ttttacgaat cccgctctca aacgtttcct
      721 gtggtttcca tcccaattcg cggctaatct tctcagcatc aatcgcataa cggcgatcgt
      781 gtcccggacg atcggcaaca taagtgattt gctcacgata agatttctct ttcggtacaa
      841 tctcatccaa caaatcacaa atagtgagca ctacatcgat gtttttcttt tcgttgtgtc
      901 caccaatgtt ataagtttca cccgctttac cttcggttac gacggtatat aacgcacgtg
      961 catgatcttc aacatacagc cagtcgcgaa tttgatcccc tttgccataa ataggtaatg
     1021 ccttaccttc cagagcatta agaattacta gtggaatcaa tttttccgga aagtgataag
     1081 gaccgtagtt attcgaacaa ttagtcacaa tggtcggtaa accataggta cgtttccacg
     1141 cacggactaa atgatcgctg gatgctttgg atgcggaata agggctgctt ggcgcgtaag
     1201 ctgttgtctc agtaaataag ggtaattctt ctgtattatt tacctcgtca ggatgaggca
     1261 aatcaccata tacttcgtca gtagaaatat gatgaaaacg gaagctattt ttcttgtcgc
     1321 tatcaagagc agaccagtaa ttgcgagcgg cttccaaaag gacataagta ccaacaatat
     1381 tggtttcaat aaatgccgca gggcctgtaa ttgaacgatc cacatggctt tcagcagcca
     1441 ggtgcatcac tgcatccggc tgatgctgag caaaaatccg tgccattgca gcagcatcgc
     1501 aaatatccgc atgttcaaaa atatagcgtt cagaatcaga aacatcagca agtgattcca
     1561 ggtttccggc gtacgttaat ttatcgacat taacaacact atcctgcgta ttatttataa
     1621 tgtgacgaac tacagcagaa ccaataaatc ctgcgccacc agtaacaagt attttcactt
     1681 aatttattcc atattacatc agagcatgct gtgaaataag cggctctttg tttgattaat
     1741 agaggtatta atgcacgcta ccgcccctgg ctttacagct accagagcac tgcatgcatg
     1801 tctacgatgt gacgagcgtt acccactcgc gctaaacccg aaaaattcaa acgctaattg
     1861 tcttaccaat ccgctctgga aacaaggaaa atcctggaaa actttgacta aaatcctatt
     1921 gctaactcgt tgttattctg attgtttata taaaacaacg gcaggaatat tcgcagcaaa
     1981 ttactttcgc cacgaatctt cactgccgtt ataattttct tatcaaccgt tacatccggt
     2041 cagattttca ttattcgctt aacagcttct caataccttt acggaaattc gccccttctt
     2101 tcaggttgcg tagtccatac ttcacaaatg cctgcatata acccattttt ttaccgcagt
     2161 cgtagctgtc tccagtcatc agcatggcat caacggactg ttttttcgcc agttcagcga
     2221 tggcatcagt cagctgaata cgtccccatg caccaggctg agtgcgttca agttccggcc
     2281 aaatatcggc agaaagcaca taacggccaa cggccatgat gtctgagtcc agcgtctgcg
     2341 gctgatccgg tttttcgata aattcgacga tgcggctgac tttaccttcg cggtccagcg
     2401 gctctttggt ctgaatgacg gagtattcag agaggtcacc cggcatacgt tttgccagca
     2461 cctggctgcg tcctgtttca ttgaagcgcg caatcatggc agcaaggttg tagcgcagcg
     2521 ggtcggcgct ggcgtcgtcg atcacaacgt ctggcagcac cacgacaaat gggttgtcac
     2581 caatggcggg tcgtgcacat aaaatggagt ggcccaaacc taaaggttcg ccctgacgca
     2641 cgttcataat ggtcacaccc ggcggacaga tggactgcac ttccgccagc agctgacgct
     2701 tcacgcgctg ttcaaggaga gattctaatt cataagaggt gtcgaagtgg ttttcgaccg
     2761 cgttcttgga cgcgtgagtt accaggagga tttctttgat ccctgcagcc acaatctcgt
     2821 caacaatgta ctgaatcatt ggcttgtcga cgatcggtag catctctttg ggaatcgcct
     2881 tagtggcagg caacatatgc atcccaagac ccgctacagg aataactgct tttaaattcg
     2941 tcattatttc atccacctgt aaaatggttg ctgaattata gcttgttcga tttttttcgc
     3001 cagcatcaat taccctgaat tgattactga attacttgtg atgttacgcc gcttcgttgt
     3061 ggattgcagt agcattgttc ctaagtatgg ctccattttt ccaggaatgg tcgcaaatct
     3121 actccctccg ttctggcaat ctaaagttaa tcttttccac attaacaata tggtgattaa
     3181 tcctgtcgat atcgacggag ctttgccctc tttcattcac cgcatgaaca ttcgcaagag
     3241 atagcagtgt ttcttttttc gccataaaga cgccgcgaac gtctttgcgc atgtcaaagt
     3301 tcatgctcaa tgctggtcca actgaggatt cctgcatcac cttgatattt cgcataaaaa
     3361 gatgttgcgg tttgttgtgt aactccagcg aggcacgctt catctcaatg ttagtcagcg
     3421 ccacaaagga gagagcattc ccggcggaga tttgaatgcc gcgcaatttg taagcaaggt
     3481 gggtgttatc cagttgaata ttattcactc ggaaattttg cggtatcgag agatatttgc
     3541 ctttaattac cccatagccg attaacatcc cggcgctatt aatcatttca atattatcaa
     3601 tcacgaaatt gtcacaaccg taaatagcaa ctgtcgcgtt atcaatgcct gctttcttac
     3661 tgaaatccgg cgtgatattg cgggctttga tattacgaat aacaaaatgt ttaccatttt
     3721 caacatggat caactgccga caatccgatc ccgtgatatt cgccacgaca aagtttttca
     3781 ctgcctggtc ttccgggtag ttgttatcgt aggtacttcc cgcaaggcct atgccaatgc
     3841 cccagttgat tttgccatta gtacagttga tgcgctcgat gacatggtcg gagatcaaaa
     3901 tatcacggtc gttaatcgcc acgttccatt caatggcatc gccctgtaag tcgctgaact
     3961 tacaattggt gatgttggca ccgattatct ggttatgaaa tccctggcgt aagatggcgt
     4021 aattagcgtg gctaacggtc aggttatcga tggtcaggtt acgcatgacc cgtttgtttt
     4081 tgccgccgat atagatctgc gtcaccgggc caaagccgct catcgtcagc cctttgatgg
     4141 tgcagtcaga accacgcaca tccagggtga tgttatgcat actgccgccc ttctcccctg
     4201 tcacctggct gccgtcctgt aagacaaatc gccctctgcc gttgcctcgc aggcttccga
     4261 ggatgtgtaa cgttttgcca ggaggaatga agatgccggt gttgatattg tcacaaacga
     4321 atccggcagg cacgacgacc gtttgccctt cgctgaaggc ttgtttaaat gaggcgatcc
     4381 agtcgtgtgg gttgtagtcg ttaatgttaa cgctctgttg gtcgggaagt gcgcgagcga
     4441 aaggggtatg gaggaaggca agcgccgagc ttgccgtcag gaacgtgcgt cgggagagtt
     4501 ttttaaatgg catgcgttct cctctataaa gcctgcagca agctggcgag ttctcgattg
     4561 atcacctgct ggttaaaatc gtgttcgact ttttcgcgcg cacgtttgac aaccggagcc
     4621 agttcgtcag tgtccagttg gctaaatgcc gccagacgtt gtgccagtgc gcgagcatcg
     4681 ttctcaggca ccagccagcc ggatttatcg gcctccacca gttccggtat tccgctatgc
     4741 agagtagaaa ctactggaat gccgaccgcc atcgcttcca ttagcgccac tggaatgcct
     4801 tccatatcac catccgcgcc cgttaccgat ggcaacagga agacatccgc gtcgtcgagc
     4861 atcgctttca cttcatggct cggtttaaag cccggcatct ctaccacatc ttccagttga
     4921 tattgttcga tgagggtgcg caggcgtcgt tcccacgggc caatgccgag aatgcgatag
     4981 cgaaatgcca cgccctgctc tttcaactga cggcaggctt caatcgccac atgcagacct
     5041 tttttctcgg ttaagcgtgc gacggaaatg atttccagcg gcgttgcggg tgctttcacc
     5101 ggacgcgggc taaagcgcgt catgtccacg cccatacgcg atacggcgat tttttccctc
     5161 ggacagccca ttttttgcag ccttccggcc cacagatcgc ttatcggtaa catcaggtcg
     5221 ccacggcgaa acagttgttg atattcggga gtgtagtggt tgagaacgtc ccgactggag
     5281 atatcaatac cgtggaagat agtggcaatt ttgccgcgaa taacacccag ttcgcgtagt
     5341 tttgctgcgg ttacccccgc cgggccaaaa tgagcgataa agacatcggc atgaaacggt
     5401 gttgcgacct ggccacaaat ggcagacaaa atcaggttcc gcgactcggc accatagcgt
     5461 ttgaggttga gcgcctgcca ggtattttta cgataaatgc cgcgtaaggt ctggctggcg
     5521 cggtggcgca atttcgctac tttgcccgca ggttcgtcct gtaaccaacg ggttctggcg
     5581 gcaaggttgt atttcgtcca tgccgcgtgg gtgttttgtg tgtcgccttt ttgcagcgcg
     5641 acaatctcca cctcaaatcc catatcaata aacgcagtga tttggttgag gacgaaggtt
     5701 tccgacgaca gcgggaattt cagtaaaaag aagccgacct tcatttcacc tccccgatgc
     5761 gctcaagcac agactgtacc atctgcattc ctgtctgacg ctcacgactg acggcttcgc
     5821 taagtcgtgc gttcagcgcc ggaagctggc ctaaggtatc cgcaaccatc gcttgcaggc
     5881 tgccgtctaa taaatgacgg atatcaattg ccatctctgg cagtcccaac tgttgcataa
     5941 tcccggcgga tttatgttcg tagttgatgg caattgccgg agtggcaaaa ttcatcgaga
     6001 taatggcaga gtgcaggcgc gtaccgacgg tgagttcaca ggcaccgaga attttgccca
     6061 tttccagatc gttaagttca tccatcacta cgtgataacg ggcaggatca ctgatgtgct
     6121 ggcgcaggtt gagcgccacc atgcggtcgt ctttgttata gctgtcgata cccgtacagg
     6181 tggagagcgc aataacctga tagccttcgt cgagaatgcg attgaccacc ccggcaaagg
     6241 ctttttcata cgcttgttga gtggtgccga gacgtttgtc gaacggtgcc agttcgcgca
     6301 gggtaatggc caccgttttc tgttgtgcgg caacgtccag ccagtgttga acggcatagc
     6361 tggcggtgaa atcttcggtg tggtgatcga ccagccacgc ggtatcgacg ccatgttcca
     6421 cttttgcggt ggtgatattg ctgcgtttca tcagatcaag gctgaccgat tcgcgcagga
     6481 tcagcgcgtc gcagtgacca aaaacgtagt tcgccagttg gttaaattgc tcatcctgga
     6541 acgggccaac actgtgacca atcataaaca gcggcttttt cgccatgaaa gtacaaagtg
     6601 catgttcaaa ctgcggcacg ccatagagat cgacaaaaaa cgatccgccg acctgaataa
     6661 tggcgtcgta acctgacagc aggcgcacga agtcggtgaa tccctgggcg atggcgatat
     6721 tacgcagctt gccagtgtcg gtgacgcgtg agagcaatac ctggtgttga tagcggcgac
     6781 ggaggacttt tttaacgcgc ccgacaacgc ccgccgcgct gttgtgttgt ttcatttgca
     6841 ggaacagcgg atcgcccatt accgggcggt tgagcagcca ggaagaactg accggatagc
     6901 ggctcatcac gtccacttcg gcttgtggat tgaggatgtt gatggcatca agtaagccgc
     6961 gcaggatggc gctgtcgcca cgattgccgc aagtgtggtt gcccagaata agtaatttca
     7021 ttttttcctc ataaatttga tgccaggtga ggctgcgttt gttttgttcg ttgccgcttt
     7081 tttcctgatg cgacgctgac gcgtcttatc aggcctacaa gacccgagcc tagaaccgta
     7141 ggccggataa ggcgtttacg ccgcatccgg caaccgttgc cggaaccgaa aacaacaact
     7201 caccccgccc gtaaaagcat tttcattttt tcgctgcgac aaaactgacg ctttacttcc
     7261 accaccagcg gatggcgcga cagcacaatc atcaccacaa acgccagcac acccgtggct
     7321 atttgcaccg ccagcagcat ccccagcgcc agttgccctt tcagcacaat gcccagcgca
     7381 taactgacca ccagcgtcgg cagcgagaga taaaacggca gccataaact caggatgtac
     7441 tggcgataac ttgaaccgag tactggttta atcatcacga aataactcag aatggtgttg
     7501 ataatctgca ccagcaggaa accgagcgtg acgccgatcg cgcccgccat ctgcccacca
     7561 ataacaatcg ccggaataaa cagaaatgtt ttgaatacgt tgaatttaaa gctgatatcg
     7621 acccgcgctt tcgccatcag cagcgaacca atcgggttac ccactgagcg cagcagaccc
     7681 acaatacaca gcaattgcag caccggaata atgctgatcc acttctcacc aaagaccagc
     7741 ggtacaaaat tattcgacac caccatcagc ccgagcagcg ccggaaagtt gataatcccc
     7801 accaccgaca gcagcttgta gaagttgacg cgcagctttt cggtgtcgtc ctgaattttg
     7861 gcaaacgccg gaaacaacac gcgggtgatg atggggttca gcttcatcgg cggcacaacg
     7921 gccacgttgt acgccaggtt gtatccccct gctacgcccg cgccgagaat acgcgccagc
     7981 accagcgttg aaaggttggt gttgagatag ttgatgatgc tgtccgccgt cagccaggca
     8041 ccaaaacgta agttcggtgc cacagacgcc agcgagaagt gcagaccggg gcaataaatt
     8101 ttgcggccaa agtagccaaa cagcagcgtt cttaccgcac tattgaccag ataaccgagg
     8161 atcgcggtca tcgccagcgg ccagaaatgg gcgctaacca ctgtaaaagt gaagcccgcc
     8221 agcaccgcgc tggtttcgat catgccgatt ttgttgaact ccagttcctt ttgcatcaac
     8281 gcgcggaact gctgcccgtg ggggatcacc acaaacgcca gcgacaatgt tttaatcaac
     8341 ggtgccaggt ccgggttatt cagcacgtca ccaatggcat cactcaacaa aaacaccgcc
     8401 acgcacacca caagccccag cccgacgttc agccagtaca gcgtggtgag ttcaaggtgg
     8461 ctgatttctt ttcgctgaat aatcgagtta gcgataccga agtcagaaag cgtatctgcc
     8521 agcgcgatga tcaccagcga cacggtaagc aaaccgaact ggtggttgtc aataatccgc
     8581 gccagcacgg tcatctgtac cagcccgagg ccgatgatga tcaccgtagc aatcgccgac
     8641 cacttcgcgc cgctgatggt tttttcacgt aagctcatat caatatgccg ctttgttaac
     8701 gaaacctttg aataccgtca ggaaaacgat tttaatatcg aaccagacgc tccattcgcg
     8761 gatgtactca aggtcgaact cgacgcgttt ttccattttc tccagcgtgt cggtttcgcc
     8821 acgccagcca ttaatctgcg cccagccggt aatgcccggt ttcactttat ggcgcagcat
     8881 gtagccttca atgagctgtc gatactgttc gttatgcgcc accgcgtgcg gacgtggacc
     8941 gacaatcgac atcccgccgg tcagcacatt gataaactgc ggcagttcat ccagcgaggt
     9001 gcggcgcaga aagttcccca ctttggtgac gcgcggatcg ttctgcgtcg cctgggtcac
     9061 cactttgtcg ttctccatca ctttcatcga acggaacttc cacactttga tcggcttgcc
     9121 atccatgcca tagcgagtct ggcggaaaat aaccggccct ggtgaactga gtttcaccgc
     9181 cagcgcaata cagcacagca ccggggagat cagcagcaag atcagcgtcg ccagcacaat
     9241 gtcttccgca cgttttatca ggcggttaac cccggaaagc ggcgtatcgt acagcggcac
     9301 caccggaacg ccgttcatct cttcgagacg tgaatggaga atgttgaagg taaaaacatc
     9361 ggggatcagc agcaccgaac aggtggtgtc cgccaattga tggaccagtt ttttcactcg
     9421 cgcgccgtcg cacatttgca tcgcgatata gacgttatga atcttacccg ctttagcatc
     9481 ctcgaccagc tgttgcaggt tacccgccca gtcgttagaa acgccgcccg gtttcgggtc
     9541 gtggtaaacg cccaccactt caaaccctaa ccacggctga ttacggaagc tctccatcag
     9601 catttgtccg gcggctaaat cccccgccac cgcgaccatg cgcttgttat agccatgatt
     9661 acgcagccag cccgccccaa tgcgaataca tgaacggcaa accaccagtc cgatactggt
     9721 cagcccatac cacgccagcc agattttcag ttgcgtgtcg aaatcattgt tgaacgccac
     9781 cagtccggca ctgaaaatca cgcttaaggt ccagttttgt agcaataggg caaattctgt
     9841 cgctgcccga acaccgcgcc atgagcgata aaaatcggtg atgccaccca gcatctggaa
     9901 caccaccagc gtaatcagcg ccaccaacag gtgcatgtag aggaatgaca gtccgctgac
     9961 ttcgcaaacc agccatagcc cggcaaacat gatggtgata tctgaaaagc gttgcaccat
    10021 agagattaac gatgcattgg ttttcgctcg ctcgcgcttt tttagatttg tcatcgttgt
    10081 tcctgttatt agccccttac ccgcagtggg gtaagggaag atccgacatt actcattcag
    10141 caacgtcagc agagttcgcg ttcgcgcttc catcagcggc acatcaccgc gcgattccac
    10201 attcaaccgc accaccggtt cggtgttgga ggagcgcagg ttaaagcgcc agtcggcaaa
    10261 ggtcatgctg atgccatcgg tgcgatccac cgccagcgcc tcacggctaa aatgctgttc
    10321 cacgcggtta atcgcctcaa tggggtgcgc cagtttgctg ttgatctcac cgcttgccgg
    10381 aaacgcagcc atccggtcgc gcaccagttc gcccagcgtt tttcctttca ggcacaccag
    10441 ttcggcgacc agcagccacg ggatcatgcc gctgtcgcag taagcgaaat cacggaagta
    10501 atggtgggcg ctcatttcgc caccgtagat ggcgtcttcc ttgcgcatac gttctttaat
    10561 aaaggcgtgt ccggttttcg acatcaccgg cgtgccgcca gcagcagtta ccacatcaac
    10621 ggtgttccag gagagacgtg gatcgtggat gatcttcgcg ccgggatttt tttcgaggaa
    10681 tgcttctgcc aacaggccga caatgtagta gccctcaata aactgccctt tttcgtcaaa
    10741 caggaagcag cgatcaaaat caccgtcaaa ggcaatgccc atatccgcgc cgtgtttgat
    10801 gaccgcattg cgggtgtcgt cgcggcattc tggcagtagc gggttaggaa taccgttggg
    10861 gaaattgccg tccggcgtgt tgtgtacttt gattaattcc accggtgcgc cgagggcttt
    10921 aaatcgggct tcaatggcgt ccaccaccgg acccgccgcg ccgttcccgg agttaatcac
    10981 cagcttgagc ggcgtgaggt ttttgacgtt gatataactg aacaggtgat caacgtaagc
    11041 gtcacgcagg ttgatttgct gatagcgacc gcgtttggtt tcatcgacgg gaggaaagtc
    11101 gttggcttct gccagacgct ggacgtcgcg cagtccggta tcaccgctga tcgggcgagc
    11161 cccttcgcgc accagtttca tgccgttgta atccatcgga ttatggctgg cggtaacttc
    11221 aatgccgcca tccacaccga gatggaacgt ggcgaaatag atctcttcgg tgccggacat
    11281 gccaatatcc agcacatcga cgcccgcatc ctgcaaacct tttgccagcg ccagttttaa
    11341 ggtttcgctg gtgaggcgga catcaccgcc taacacaatg gttttcggtt tgagaaattc
    11401 gccataggca cgcccaatac gccaggcaat atcttcattc agttcttcgc ctaatttccc
    11461 gcgaatatca taggctttaa agcaggttaa ttttttcata tcgttaccct ttttcaggca
    11521 atagttggcc ctgaccgaaa cggccaattt tgtttttgtc gttatttaca ccgcggtttc
    11581 gcattcattg cctgatgcga cgctgccgcg tcttatcagg cctacaaaac cgaaccgtag
    11641 gtcggataag gcgttcacgc cgcatccgac aaacagcgcc tgatgcgacg tttacacccg
    11701 tccgtagcga tccgcgaaac gcaccacatc atcctcttcg agataagagc cggagcgtac
    11761 ttcaattaaa tcgagcggaa ttttccccgg gttttccagg cagtgcgtcg cccccagcgg
    11821 aatataaatg gactcgtttt caccaagcag tttgatatca ccgtcaatgg tgacttttgc
    11881 cgttcccgca accaccaccc agtgttccgc gcggtgatgg tgcatctgta ccgacaagcc
    11941 ttcgcccggt ttcacggtga tgcgtttcac ctggtagcgg tcgcccgcgt cgatagagtc
    12001 atatttgccc cacggacgat acacttcgcg atgcacccga tgctcatggc gaccatcggc
    12061 tttgatctgc tcgaccactt ttttcacatc ctgtaccgcg ttacggtcgg caatcagcac
    12121 cgcatctttg gtctgcacca ctaccagatc tttcaccccg acggtggtga ccaggccaga
    12181 ttcggcatac acatagctgt tttcagtttt gtgattaatc acatcgccgt ggcaaacgtt
    12241 gccctcggcg gtgtgggcgc tgatctccca taatgaagac caggaaccaa catcgctcca
    12301 gcccgcatcc atcggcacca caacagcatc tgccgtacgt tccatgaccg cgtaatccac
    12361 cgactcttcc gggcaggcga gaaacgcttc ttcatccaca cgaataaaat caagatccgg
    12421 atcgacggcg ctcatcgctt tttcacaggc atcgagaata tccgggcgat attttttcag
    12481 ttcttcgaga tagcgtccgg cgcggaacag gaacatgccg ctgttccagt aatattcgcc
    12541 gcttgccaca taggcctggg cggtttccag attcggtttt tcgacaaact gcgccacttc
    12601 aaaggccacc gtatcctgct cacccgcaga cacttcaccg cgacgaatat agccataacc
    12661 ggtttctggt agatccggca caatgccaaa ggtcaccagc ttgcccgctt cggcatatgg
    12721 catggcatta cgcacggcgg cacggaacgc gtcttcatcg gcaattacat gatccgccgc
    12781 cagcaccagc attaacgggt cgttctccgg gctatgacgt tttgccgcca gcgccgccag
    12841 cgcaatggcc ggtgcagtgt tacgccctgc aggttcgaga ataatgttct cggtaagttt
    12901 gttcagttga cgcagctgtt ccgcgacaat aaagcggtgc tgctcattgc aaatcaccac
    12961 cgggctttcg cactccacgc cgttcaggcg gcagatggtg gtttgcagca tggtgagatc
    13021 gcctttcagg cataaaaact gcttggggta aagtacgcgg gaaagcggcc ataagcggct
    13081 accggagcca cctgccatca caactggata gagtttcgac tgcgccataa ttatccccga
    13141 atatcattta taaattgacg taacacgttc tctttatcga gcgtgcgttc ggcatattca
    13201 cgtgccaccg tgttgtactt gggcagcagg agcgcctgac gaatccccgc caccagcgcc
    13261 tcgaccgatt ccggttccac gcaaaccgca atgcccggaa aggtttcgca aagctgtccc
    13321 agttctgtgt gggcctctgc ggtaatcacc gcgttaccgc ctactgccag aatattggtc
    13381 agtttcgacg gcaatacggc atctgccgcg ccgcgttttt gcaccaccag atggcaatcg
    13441 cccatcttca gcagtgcggg taaagcgtca tacgattgca gcgggaaaaa ttgcatgttg
    13501 cgcagtccac gctgctgcgc cattttttcc agccgcgctt tgccgccgcc ctgcccgaca
    13561 atggcaaaaa tcagcggttc atcgcgcagg cgatcggcag cttcaataac gttttccagt
    13621 ccctgctttt caccaatatt gccggagtaa agaatgattt ttttgttatc cggcaggcca
    13681 agctggttac gaagggcatc aacatcggcg tctgcaacat gctgaaaacg ggcgatttcc
    13741 gaccagttgg ggaagaaaat gatgttatcc gccgccacgc ctttttcgat ggctttattc
    13801 atcatcgaac gcgaaatcgt ggagacgtta tcgacgttat gcagtccgct acgttcgaac
    13861 gccgttgcca gctgtgccac tttgccgcct ttgccttttc cggcaaggcc cagccccagc
    13921 atggcgtcca cttcgtaatc ctgaatatgc agcacggtac gcgcaccaga gagtttcgcc
    13981 agcaggcgca ttcccggcgt gcaaaacagc gttggcacca cgccgataat gcgatccggc
    14041 ttccagcgac gttgcgccat caacgggaaa aaactgctga cggcaaaact gccgagatgc
    14101 aacaggcgtt tcagggtgct cggctgtttt ggcacgtaca gcgggcagcg ccacaccgtg
    14161 gcggccccct cttctcgttt gtagcgccag gcggaatagt tctcgcccac ctgccactgc
    14221 gggtagtaag gcggtgcggt aataacccgc acctcatgac cttgcgccgc cagccattcc
    14281 accatttctc cggtgtattt gccgatgccg gttaactccg gcgagtagtt aatgccgtag
    14341 accagtattt tcataatccg ggtactccgg cacgcttctc agcgagaaaa taggcgcggc
    14401 tgttagcgtg aacattgtca ctggcgagca gtgcgtccgg cgtcagccag cggtaatcgt
    14461 catgctgctc atccggcagt aacagttctt cttccgctac tctgaagcga aaaccgagca
    14521 ccacatagtg agtggtgaaa tccgtgccgg agaagttatc gtcataaaag tgctgccaga
    14581 caccgtaaaa ctggcctgct gttatcggca gacgcagccc cagttccgcc atcgtcagcc
    14641 gctcaaatgc ggcttccagc gtttcgtctt tctgcacgcg ccctcccggc acaaaccagt
    14701 aaccctgcgc cgggcggttg gttcttttgc caagcagaaa ctcgccgcga ctgttctcga
    14761 caataaagtc gagagagaca agcggtgtgg agcgcactac agtggcaaag tcttcctgac
    14821 gtaaaaacat cattcccccc gaaagcggtc ttgattctca aggaaccact ggtaagtgct
    14881 ggcaagcccc gcttccagtg agatttcgtg ataccagcca agctgatgca ggcgcgtcac
    14941 atccagcagt ttgcgcggcg taccatccgg tttgctggca tcaaaaacca cccgaccttt
    15001 gtaacccacc actttggcga tggtttgcgc cagctcgcgg atagtgcagt caacgcccgt
    15061 accaacgtta atgtgcgaca gcatcggctg ggtgttctcc agccagactt catgcgccag
    15121 ctccatgaca tgaatgctcg ccgccgccat gtcatcgacg tgcaggaatt cacgcatcgg
    15181 tgtaccgctg ccccatacca ccacgtccgg cgcattctgt gccgtcgcct cgtggaagcg
    15241 acgcagcaat gctgggatca catgcgagtt actcgggtgg aagttgtcat gcgggccata
    15301 caggttggtc ggcatgactg agcggtaatc gcgtccgtac tggcggttgt atgattcgca
    15361 cagtttgatc ccggcgattt tggcaatggc gtaaggctcg ttggtcggct ccagcgtgcc
    15421 ctgcaataac tcgctttctg ccatcggctg ttttgccagt ttcgggtaga tacaggacga
    15481 tccaagaaac agcagtttgt tcacgtcgtt ctgatgcgcg gcgtgaatga tgttgctctc
    15541 aatcatcatg ttctggtaga tgaaatccgc cggataggtg ttgttggcaa caatgccgcc
    15601 cactttcgcc gccgccagat agacctggtc aatgcgttcg ctggcaaaga aatcatgcac
    15661 cgcgcggctg tccagcaggt tcagctcgtc gcgggtgcgt aataccagtt ccacatcacc
    15721 gcgctgttcg agctgccgcc tgatggcaga accgaccatt ccgcgatggc cagcgataaa
    15781 aatgcgttgt ctactcatgc ttatgactcc agcgcgatcg ccacgtcgta gccgtgagat
    15841 ttcagcagag agtgtttttt cgccgcttcg aggtcattag ccaccatttc agacaccatc
    15901 tctctgaggg tgatttccgg tttccagccc agtttttcgt gcgctttggt cgggtcgccg
    15961 agcagcgttt caacttcagc cggacggaag taacgcggat caacggcgat aatcacatca
    16021 cccggtttaa cgcccggcgc gtcatgcccg gtgacggaaa ccacaatgcc cttctcttca
    16081 acacccgtgc cttcaaagcg cagtttgatg cccagctgtg ccgctgccat ttccacgaac
    16141 tgacgcacgg agtactgaac gccggtcgcg ataacgaaat cttccggctg ttcctgttgc
    16201 agcatcatcc actgcatttt tacgtagtct ttggcgtggc cccagtcacg cagggaatcc
    16261 atattgccga ggtacaggca cgactccagc ccctgggcga tgttggcgat tgcgcgggtg
    16321 attttgcggg taacgaaggt ttcgccgcgg cgcggggatt catggttgaa gagaattccg
    16381 ttacaagcgt acatgccgta ggattcgcgg tagttaacgg tgatccagta ggcgtacagt
    16441 ttggcgaccg catacggaga tcgcgggtag aacggcgtgg tttctttctg cggaatttcc
    16501 tgcaccagac cgtacagttc agaggtggaa gcctgataga aacgggtttt cttttccaga
    16561 ccgaggaagc ggatcgcctc gagcaggcgc agcgtaccca tcgcatcgac gtctgcggta
    16621 tattccggtg actcaaaaga aaccgcaacg tggctcattg cgcccaggtt gtacacttca
    16681 tccggctgca cttcacgcaa aatgcgcgtc aggttggagg tatcactcag gtcgccataa
    16741 tgcagatgga atttcgggtt gcaggtgtgc ggatcctgat aaatgtgatc cacgcgctcg
    16801 gtgttgaatg acgatgcacg acgcttaata ccatgcacct cgtaaccttt ttccagcaga
    16861 aactctgcca ggtaagaacc gtcttgtccg gttacaccgg tgatgagagc gacttttgac
    16921 atgtcttatt cctctgtatt tttgaattta ttcagtttca acgcgttcgc gtatcaccac
    16981 tgcgggattc ccccggcaaa ccacatttgc cggaagcgat ttaaaaacac tgcttcgtgc
    17041 acccacgacg gtgccgtcgc cgattgtgac gcctggggca acaaagacat cggttgccag
    17101 ccagcatttc tcgccaatca caataggcgt ggcgttaatg gtgaaatgtt gacttgcatg
    17161 gtcgtggctc ccggtgcata aataactttt ttgcgatatc accgaatgtg cgccaatggt
    17221 tatttcaccg agggtatata aattgacctc atcgccgacc cacgcgtaat cacctaaggt
    17281 taatttccac ggataggtaa tttttactga cggacgaata actacgtttt ttcctatttt
    17341 tgctccgaat aaacgtaata aaaaagcccg ccagcgatac aatacttgtg gcgaccaggc
    17401 aaatattgtt gcctgtactg cccaccataa ttgcacttta atagcgttgc cgccccggaa
    17461 ccctttcggc accgagaatc cacttaaatc ctgcatcgtt ttcccttata ttcagacttt
    17521 gttatataag gctttcgtct ttgaggtagt acgttggcgt aaatgccagg ataattcagc
    17581 ccaaaagcca ggtacatgta atatttgtcg ttggactttt ttcgcgtcag cacacaattc
    17641 catattattg gtggtagata ccccacccat ggaaaattca gacaccaggc cattgagttt
    17701 tttaaatgca taaccggctt tatacatttt ggctgccagc gcgtagtcgg aagaaacttt
    17761 atattccagg tcataacgcc atttcttcaa gccggatacc gggaaaaata tcgcctgatg
    17821 actggcgggc aggctgtgat aaatatacca gcccggtttg gcgctacgtt taattttatg
    17881 cccgtcgcca aaatccagca gcgcatcgcc ggtgatcatc acgttatctt tttgcatttt
    17941 taacttacgg acaaaatttg cggcattctg atgaaaaata tcgcccgaat tgagaaacaa
    18001 cgcgaacttg ccttgtgcca tcgtaatgcc tttgttcatg gcgtcataga taccgttatc
    18061 tggctcgctg acaaagcgta ggttatagat accattgaga ttttccagat actcacgggt
    18121 gccgtcattg gaaccgccat cgacaacaat ccattcgaag ctgatatctt ccgcctgcgc
    18181 cagatgcgcc agcgaggcat gtgttttgac tatcccttcg aggttacgaa acgcgacagt
    18241 gattatgcta agcaacatgt tcttattctt acctcgtaat atttaacgct tttcgcaaaa
    18301 taaacggaca gacgattaaa aatgcatatt ccgggctaaa tatcgaaccg gtaaaaaaca
    18361 gcgataccgg agtaaaaaga tataattgca cgcgaaaatt gcggttatca ccaaaagcgt
    18421 tgatcatcat ttttatcact ttccccatgt accacagcga taaaaacacc gcgaaccagg
    18481 aaaaataaat aatcagcaga tacaaaccat tgtctatggt ttttccgaca tccgcaccgt
    18541 taaatattcc gaatgatgcg acatattcat aaagtgagcc aaatctgact acaccgtcaa
    18601 tatgggtcaa agaataaccg accatcacca gcggaccgac aatacgataa tacgatgacg
    18661 atccttccgt acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac
    18721 caaccaggaa tacagccaga gaaatcaatg ctaacggtaa ctttttctta atcgcctctt
    18781 tattcagata ttggaatgcc cactccagca aataaaacag gataaaggtc ataacccctg
    18841 aaaacgatcc ggataatatt atccctgcga gaatcatagc atcggtttta ggcgttttga
    18901 taccaaactg tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt
    18961 cgaaataaag tgctgtcgtg cgcttgccgc cgaatttgat gaaattcagc acatagctgt
    19021 tgctgtaaat cagatatttc gaaatcgact ccataatact gctgccgccg gtgaggataa
    19081 tttgcgccat ctccaccgcc gccagcgcca ccaccagccc gaccaccaga taaaagaaac
    19141 gtaatatctt gcgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc
    19201 caataatgat cacaatatag acaaacagca tggttgaagt gacgtattta ccggcatcca
    19261 gcgactgacc aaacaggtag ttaaacgccg tgagtcccgc accaatccct aaggcaatca
    19321 tcaatttttt aacgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga
    19381 cgatagtgat ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga
    19441 tcagcggcag cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc
    19501 tgttgtccac tgtaggcggc gcggctgcgt tggctgaact cagccagcgt ggtaccaaat
    19561 atcgcctgcg cgatttccgg tttgcttaac tgcaccagtt gcagcacctc ttcttcactg
    19621 acggttttac cgccggattt ttgcagcact tcacgcgccg catcgctatg ggtggcaatc
    19681 accggcacgc caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacgcgagaa
    19741 ctgaacacca gcgcatccat ctgattgagc gcgctcatca gcttacgttt gtcggtttca
    19801 aagccgtgat taaccacgtt accagcggtg aacggcgaga acttaccaaa ggtatgcagt
    19861 tcgattttgt cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg
    19921 taacgcaggt catgcgccac caccgcgatt ttcggcttac cctgggtttc gcgcaccgga
    19981 ggcagatccg ccagaatcgc ttcggttgcc atatcaatac cgttattgat aatccggcaa
    20041 cgccctggac cgtacaggct attgaaagcg tcagccacat gctggctggg ggaaataaac
    20101 tgacagccca gcgccagcat ctcacggaat aactggcgtt tgcccgccac cagttggtgt
    20161 gcgcgatcaa tcttcaccgg cggataatta tttaaggtcg ggcatttctg gcagcccgtt
    20221 ttccagcctt cacaaccgtc ggtaaaggcg cagcgtccgg taacgctcca gtggtcgtgc
    20281 agcgtccaga ccagagtgac gtccggttta tggtttttca ccttttcgca aaagcgcacc
    20341 acgctcttaa gatttagcca gtagctgtgc agcacatgaa aatgcaggac caccggaccc
    20401 ggtgtacgag taatggtgcg atataactca ttgaaattgc caaacagatc gcgattaaac
    20461 agacgaaaca ggacaatatt cgccattgca gtcatccgcg gcgtatgttt gatgacctgc
    20521 ggatagtttt gatggctgac gctctctttg ccgcctttgc cgtaaccgta gacaaaatgt
    20581 gacgctagcc cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg
    20641 ccttccgcca gtcgcacatt aaattgcaaa atattcattt aattaccttc actcgcgctt
    20701 tttctcccac caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca
    20761 cggtgacgtt gttaccaagc gtgatatcac caagaataat aacgttggca ccgagttcga
    20821 cgccgttgcc aatatgtgga catgccatgt tatcggcacc acgattgccg atagtgacgc
    20881 cgtggcgaat ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac
    20941 cgtgatggat agtaaagcgg cggccaatgg tcgcggcagc ctggatttca taaccgaaaa
    21001 agcattcggt gataatgcga tacagcacca gcagaggggc cgcccaaata ttgttgagga
    21061 cgttcttttt gcgccacacc gaacaaaaat gagcgacgcg ataggctaga accatgcagc
    21121 acggacgtaa actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat
    21181 accatcagcc agacgtttgc cgttacgcac cgacagcagc gtcagcaaag tgcgccaggt
    21241 catacgttta ttgcggatct ggtagagggt aaacagctga tattttttgc tggcgcggtc
    21301 gaatttgtct ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga
    21361 ggtgatctgc atttcaccgt gattgatatg caggatctgc gtcgcctctt ccactttcca
    21421 cggttcgcca tactccacca ccatccgcag gaagatgtcg taatcctgtg ccgctttcag
    21481 ttcggtatcg aacaagcact ctttgaaacg ccacgcccag gtaaaaacct gattgccaat
    21541 gatattgcgt ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct
    21601 tgctggttgg gaataaactt ctccctggca tacgtagtcg ttagcgtaca aaaaggcgtg
    21661 agtgaccagt tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc
    21721 atcgtcatcg atcccggtaa tgtattcccc ttgcgccagc ataatcgcct ggttacgtac
    21781 cgcgcacgcc ccgctgttaa tgtcgttgtg aatgtaggtg atacgcggat cgttgagggc
    21841 agtgacgtac tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc
    21901 ccagttgctg tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg
    21961 gttccaggtc ggcatataga ttgaaatcag cggattgtct ttcataggtt gttctcccgg
    22021 agtatcgaga tgttctgccg gtttataagc ttgtttacgg gttctgttgc cggatgcggc
    22081 gtgagcgcct tatccggcct acggagctgg tttgcttgcc ggatgcggcg tgaaggtagg
    22141 tcggataaga tgcgtcagca tcgcatccga cagcggatgt cgttatttcg catccgactt
    22201 atattcgtat tcgtaatagc cataatcctg atacgcgctg gcgcggcgga agatggagtt
    22261 cagaatcacc cctttcaccg gaataccgtt ttgctcaaag cggctcagac tggtttccac
    22321 ttctttcaat gtgttgaccg cataacgcgc caccattaac gtggttccga catgacgacc
    22381 aacaattgcc gcatcggtca ctgccagaat cggcggcgta tcaatcaaca ccaggtcata
    22441 gtttttactc gcccagttca ccagttcggc aaagcgttcg ctcatcaaca gttcagaagg
    22501 atttggcggt acctgaccgc gcgggatcag gtcaaatttg gcaatagagg tcggtttagc
    22561 tgcggtagta atatcgccct ggccaatcag aatttccgac aggccattga cgttattggt
    22621 acccaacagc tcgtgggtgt agcctttgcg catatcgcag tcgatcaaca acacccgttt
    22681 attggtctga ctgatcaccg ccgccaggtt ggcgcagaca aaggttttac cgattgacgg
    22741 gctcacccct gtcatcatca acacattgtt ctgcgcctgc atcatcgcga agtgcagact
    22801 ggtacgcaga ctgcggatgg cttcaatcgc cagatcggtt ggattcccca ccgccagtag
    22861 ctggctctgt ttatagcgtt taatcccttt gatggttttg acgctatcgc gcgctttctg
    22921 ccattccgac aacgggatgc tggcatagac gctgatacca tgttcttcca gcacctgcgg
    22981 gctttcgatg ccgcgattaa acaacgagcg cagcagcaca cccacgatag agagcatcag
    23041 gccgaggata attgcgccga ggataatcag tcctttcttc ggtttcagca cgccaggctg
    23101 ggtgattgcc gggtcaacaa tgcgcacatc gccgacggtg ctggcttcgg tgattttcag
    23161 ctcctgctct ttattcagca gttgcatata gacctgctga ccagactcga catcgcgggt
    23221 cagacggaca atttcctgct gggttttcgg catcgccgtt acgcgaccat taagtttggc
    23281 tttttcgtct tccagcgcct gacgtttctc cagcagcgtg cggtacgcgg ggtgaacttt
    23341 ggtgtacagc ttggagattt ccgcctcttt aaaggtcagt tcgttcaact gcgcgtcgat
    23401 gttcaccatc gaatcgagca ccgctttcgc ttccagcggc agatcaacag aatctttatc
    23461 ctgacggaag gcattcagtt tgttttcggc aacatcgagg cggctacgta cttccggtaa
    23521 ctgttgcgcg aggaaagcga ggcttttcga cgcttccgcc gatttgcgct caatattttg
    23581 ttcctgatag ttacgggcga tgctgttaag aatgtcgcgg atctgttcgc gatcttcacc
    23641 ggtataagtc aggctcagta cgcctgcgtc tttgccgttc tccgttaccg tcaggctgtt
    23701 ttgcagttgg ttgatcatcc ccaacgtgga gtatttggtg acggtaaact cactgcccgg
    23761 gcgggcgtga atggcttcaa ccatcagcgt gacgccttct tttttcagaa tctggcccgc
    23821 ttgcccacgg gcgctaaagc cgccatcact gctcagagtg tagtttttgt catccagcac
    23881 attaagcgta aacacctggt ccgccatctc tttcgggcgg ttaaaggtgg tcactttcac
    23941 cgtctcgttc tgacgtccca tcaggcgatc ccaacccgca ccgaaaatcg ggaacgtgtt
    24001 tttgctcacc gcaatatcga ggtctagatc gtccaccgtt ttaccaagca ccagacgcga
    24061 acgaatcaac tggatctcgg cgtccgatgc aggcggtttg ttggctaatg ccgagccaat
    24121 gtcctgcact aacgaattgc cgctgttttg ttcgatttgt accagtgcgt cggcactata
    24181 aatcggcgtg gcgaagaaag tgtaaaccac agcacagagg gcaaatacag cggtgatgcc
    24241 aatcacccac cagcgcgctt caatgacggt gccgaccagg cgaccaatat cgatttcatc
    24301 actgcccgtt accggagcgg catgttgttt tactttttct gtcattctta tacctgctct
    24361 gcgttcaatg cctgcgccca ctggcgggca gaccgttcaa gtaatgtgta caccgctgca
    24421 aacgtttccc ggcttttgcg atacggatcg gggatttcac attcgttatc ccagtgacca
    24481 aacagcatca ctttgccgcg catctcaggt gccatctcgc ataagcgttc gatatggcgc
    24541 ttttccatgg tcaaaatcag gtcatagttg cgacacaggc ggcggctgat ttgccgtgca
    24601 cagtgacctt ccagagacag ttgatgttct gcggcgacgc tgatagcggt aggatcagcg
    24661 cccttaccga ccagcgcgcc gagtccagcg gactccactt tcagctccgg gtgataacgt
    24721 tgcagtaagc gttccgccgt cggggaacgg caaatattgc cgacacagac aactaagatg
    24781 ttgttaaaca tgacgattac cagttatgaa tgtcgctggc tgtatccgtc atgtaacgga
    24841 caccgctaat agttggcagc aactgattga tcagacggtt ccagcgggaa accggggcgg
    24901 tggtgacata caccacgtca taaggttgca ggcggaattc tgttgccatc accagcgacg
    24961 tcgcatcgga catatccagc tggtagatat tggcaatctt gccgttacgc ccgccctcgc
    25021 ctttcagcgg acgaatgaca aagatgccgc tggcgttgga ggtggtcatg tcgatacctt
    25081 cggcattgcc cagggcttca gtcagggtca tgccgctaaa gtccattttc agggtgctct
    25141 gtttcttcac ttcacccatc acaaacactt tcagatcatc attacgcggc acgtagagaa
    25201 tatcgccggg gtaaagcagt cggttctgat tgaggtcgcc gttttgcatc agcgcctgca
    25261 aagaaatgcg ttcttcacga ccattgtgtg ttagcaccac gttgcgccag tcagcggtgt
    25321 cggtcaggcc acctgcggcg ttgatggcgt cgagaatggt cagtggtacg ttggtgatcg
    25381 cctgttgacc ggatttattc acctgacctg agatataggc cttttgcgag cggaaggcgg
    25441 cgatattaac atccacctgc gggtcagcga tgtacgtcgc taagcgcccg gtaatatcac
    25501 tgcggatttc agcgagcgtt ttcccgacta cgtggacctt gccgatatac gggtaaaaca
    25561 tagtgccgtc aggctgtacc cagttgccgg tgtcgctgga gctgcggtac tgaccggctg
    25621 gcgtggtgag ttccgggtga tcccagacgg tgacattaag aacgtccccc ggcccgacgc
    25681 gatactggta attcgcgatc tcactttcca gcgtcatatt ggggcgcgct acattcgggc
    25741 gtggacgtaa ttgatcaatc aggcgcgggg tcaacggata aacattcacc attttgtcga
    25801 gatcgaaatc agcgtcctgc tgtttgatga cgtctttgcc catcgtcgac atattgctgc
    25861 ccggaagtac tgtgcaaccg cttatcaagg ttactgacac caataatggc atcaatttca
    25921 ttttggattt catcattgtt tatttatcac tttggcagag taattatcct gtgcactatt
    25981 aatagcaatg tcgccatgca catttacctt gcagttaatt gaataaaaat ttaactggca
    26041 tcagtcctaa aaaaattgat ttcatccgca ggctattgac agaataattc agactggtct
    26101 ttcaggcatc cagacacgct accgcccctg gctttttagc taccaataca ctgatttagc
    26161 ttaatttttc aaaccctctc tgcatacaat cgttgatgag aaagggttat tgcggaaatt
    26221 aacttccgaa tataaggtga cattatggta attgaatatt ggctctccaa taatgcagga
    26281 ggaagtgtta cagctaacgg aatagcaggc aaggtaacaa ttcggcaatt ggctattttt
    26341 aagaattata ttaagtgtca ttcaatatgg tttttaggag tttctttagg ttgacaatat
    26401 ttaatattgc gcctccacat gcgatatttc ttaaataatg ttttattatt accactttta
    26461 attcagggat aatgtgaggt tattaccctc aaataatatg aggtaaatag tgctgcaaca
    26521 ttgcattttt gccccgattt atccatgatc gaattgtgac atttgtcata caacgaatag
    26581 gttttgtact tactatggaa tggattgccg atccgtctat ctgggccggg ttaatcacgc
    26641 tgattgtgat cgaactggtc ctcggcattg ataacctggt ctttattgcc atcctcgccg
    26701 aaaaactacc gccgaagcag cgcgaccgcg cacgggttac cgggctgctg ctggcgatgt
    26761 taatgcgcct gttactgctg gcgtcaatct cctggctggt caccctgacg caaccgctgt
    26821 tcagcttccg ctcgtttacc tttagcgccc gcgacttaat catgctgttt ggtggtttct
    26881 tcctgttgtt caaagccacg atggagctga acgaacggct ggaagggaaa gacagcaata
    26941 atcccacaca acgcaaaggt gcgaagttct ggggggtggt gacacaaatt gtggtactgg
    27001 acgccatctt ctcactcgac tcagtgatta ccgccgtcgg gatggtcgac catttactgg
    27061 tcatgatggc cgccgtggtt atcgctatca gcctgatgtt gatggccagc aagccgttaa
    27121 cgcaatttgt taacagtcac ccgaccatcg ttattctctg cttaagcttc ctgttgatga
    27181 ttggctttag cctggtggca gaaggtttcg gcttcgtcat tccgaaaggc tacctgtacg
    27241 ctgccattgg tttctcggtg atgatcgagg cgctcaatca gttggctatc tttaaccggc
    27301 gacgttttct ttccgctaac cagacgctac gccagcggac taccgaagcg gtaatgcgcc
    27361 tgcttagcgg gcaaaaagaa gatgcggaac tggacgccga aaccgcgtcc atgctggtgg
    27421 atcatggtaa ccagcagatc tttaatccgc aggaacggcg gatgattgag cgggtactta
    27481 atcttaacca gcgtactgtc agcagcatta tgacgtcgcg ccacgatatt gagcatatcg
    27541 atctcaacgc gccggaagac gagatccgcc agttgctgga gcgaaatcag catacgcggc
    27601 tggtggttac cgacggtgat gacgcagaag atttgctcgg tgttgttcac gttatcgacc
    27661 tgctacaaca gtcactgcgt ggcgaaccgc tcaacctgcg ggtgttgatt cgccagccgc
    27721 tggtgttccc ggaaaccttg ccgttgttac ctgccctgga gcagttccgt aatgcccgca
    27781 cgcactttgc ttttgtggtg gatgagtttg gctcggtgga agggattgtg acattaagtg
    27841 acgtcactga aaccattgcc ggtaacttac cgaacgaagt ggaagagatc gacgcccgcc
    27901 atgatattca gaagaatgcg gacggttcct ggacggcgaa tggtcatatg ccgctggaag
    27961 atctggtgca atatgtgccg ctgccgctgg atgaaaaacg tgaatatcac accattgccg
    28021 ggctgttgat ggaatattta cagcgtattc caaaacctgg cgaagaagtt caggtggggg
    28081 attatttgct taaaacgttg caggtggaaa gccatcgcgt gcagaaggtg cagattattc
    28141 cgctgcataa ggatggcgag ttggagtacg aggtgtgatg gtatcggtca ttttacccga
    28201 tagccgaaag gaataagatg agggaaccgc gccagcggcg atttaccgcc gggagcccgg
    28261 gttgccaggg tggtggcgat tgagccaccc tggcacgttc accggcttga gcgttccata
    28321 gtagtgagga acatcaagtg aacggaacag ccttcgatct tacatattca gaatataatg
    28381 gtgccgccgc gaacgcctga tgcgacgctt tacgcgtctt atcaggccta caggacaggt
    28441 cacctggtaa cctacatctt ctccagcaac ttcttcacat ctttgccatt gcgggaatct
    28501 ttattccgct cggcccagtc attcaggcga cgtttcgctt cgtcctgtag atgtttgcgc
    28561 agtaactgat ccacttgcag actgtaattg agttgctgcc agttgccata aacccgcagc
    28621 ggtactggcg tttctttgag gaaatcaatc agtttgcttt ccccgttcca gccacccacg
    28681 acccgaatat caaactgggt gtcgcaggtt tgatctgcca gattcaacat gccttcccct
    28741 gtcagcgcca gcacaggcga ttgaccttgc atgtcgttta acgtcacgac gccatccttc
    28801 aacgtcaaat cggtggtaaa gcggtcaagc cgcgtcacgt tatcaaagtt ttcagcggcc
    28861 ttcacatcac cgccattacg ctctaccgct tgctgaatca tctgctggaa gttcatccct
    28921 tccatgcgcg tgtcggtcat ttcgacatgt gcctgtcctt gccagttgtg gcggaatgcg
    28981 tcggcatcta tgtcagcacc ggagaaatca ccagccagtg acatttttcc ggtcagcgaa
    29041 atggggtagt taaaggcatt cagaatggta ccaatctcaa cgttttccag ccgtggctgg
    29101 aagtttatcc gcggatttat tgatgtcgcg tccagcgtgc ccggcagtga aacctgtcca
    29161 ccgttaagct tgccctgcag ttgagtaatt tccagcaaac ccgacttgtt ggtcatttgc
    29221 gtggcaacat ctgtaaaatt cattccgcgc cagcgcacgt tactggcctg caacaaaata
    29281 tcagccgtaa agccttgcag tccctgatat gccggttcat caatacgcga agaaatgacc
    29341 gggcgcggca atgttgattg gctctgcccc tgctgcgcgg caccgttttc accattcgct
    29401 gtttcattaa gcgggatgag gttgtcaaga ttcagttgcg ggaattgcag tctcagctgc
    29461 cattcaggtt tctctgtcag cgtgacctgt gcttgcccgc tcagcgtgct gtcattggcg
    29521 gtcaagctga tttggttaaa tgaaaggcgt ttatgtgact cctgccactg ggcctggaaa
    29581 ctcccctgcc cctgaatacc ttgttttggt aaatctgcac cctgcaactg ccagttaatt
    29641 tgttcaatag ccgccgttaa atcatgcgga taatcagaag catctaccgt accgttaagg
    29701 gatatcgtga gatcgcgctg atcgcgatta acccgcccgg agaactcaaa tgaaccacga
    29761 tgttggggat cttgttccat ttgcaggcga atattgcgga ttgtcacctg ctcgtcatct
    29821 tcatgctgga acaccagcac gctgtccgcc accttaagac tggatatatc aaacgaccat
    29881 ccgcgatcgt ctgacagatc cggcaaggta ttgtcgcgcg gtgcaaccgg agcgtcttca
    29941 ctgcgcaccg cttccgtctg cggcgtcagt tggatcactg ccccttttag catcacctgc
    30001 ttaacgctca gttgatgact cagtagtggt aaaagcgcca cgtccagacg catgttgtcg
    30061 gcgcgaacca gtggctggct tgcgccctgg gcggtgagag acattcgccc ggagaggata
    30121 ctaagctgcg gccagacatg ccaacgcagt ggcccgtcga gctgcaattg ataaccgcta
    30181 cgtgcagcaa cttgcttgac catatagtcg cggaaatcat tcggattcac cagcaacact
    30241 aacgcagata acccggccac cagcacgacc aggagtatca tcagcgtcgt cagaaatcgt
    30301 ctcatggtat cctcaatggg cctgaattag tctttatcga ttcggctggc taccgcgccc
    30361 tgctggttgc gatatttcgc atcttcacgg cggttgtaag gtcgcgccgc cgggccggaa
    30421 agcggctcaa agctcagcgc accaattaac atgcccggac gcagcgccag cggcagctta
    30481 ccggagttgt agaactccag cacaatgcaa ccagaccagc ccggatcgat acggtgcgcg
    30541 gtgacgtgca ccatcagccc cagacgcgcc aaagaggaac gcccgtccag ccagcccacc
    30601 agatcggctg gcagcgtcac cgactccagc gtcaccgcca gcgccagctc tcctgggtga
    30661 agatagaacg cctcgccctc gtcgagaacg atctcatcgc tcatcacgcg gtcaagcgcg
    30721 gcgctcactt catctttggg accgctcaga tcgataaacg ctgccgtgtg accacggaag
    30781 gtacgaaatt tattgcccaa gcgtacatcc accgtcgcgc cgttaatacg ctccactggc
    30841 ggacgtgggt tgatcgacaa acggccttca tcaagccagg cttcaatatc tcggtcacac
    30901 agacgcatgg cattttctcc tttcgcgcat cactccctta acgccaatta cgtcaagggc
    30961 ataccaggtt atcactgaac ggtacacaat ttatcaagct tattcaaaga actgacttat
    31021 tttcgctttc aatatatcga tcgcgatgcg gtttttcccg ccgcgcggca cgataatgtc
    31081 cgcatattgt ttagaaggct caatgaattg caggaacatc gggcgcacgg ttttttgata
    31141 ttgcgccatc acggaatcca ttgaacgccc acgctcgtta acgtcacgct tgatgcggcg
    31201 catcaggcag atatccagcg gggtatcaac gaaaatggag aagttaagtt cgtcacgcaa
    31261 acgcgcatcc gtcagcaaca aaatgccttc gagaatgatg accttcttcg gctcaaccgt
    31321 caccgtttct ttcatacgcg tatgttcaac atagctgtaa accggcaggt caattgccga
    31381 gccgcgtttc aacgcttgta aatgctcaag cagcaggctg tgatccatcg cgctgggatg
    31441 gtcgtagttg gtcttaacgc gttcttccat cgacagatgg ctttgatctt tgtaatagca
    31501 gtcttcggga attacgccga tgtgttcatc accgacttgc tcacgtaatt cacgataaag
    31561 ggtactggca ataagactct tgccggaagc cgatgcgcca gcgataccga taatgacgca
    31621 ctgatgagac tgatcagtca tatatttagc gacctgatta acctggatgt taggaagggg
    31681 gcgacgaagc gccaaacgcg gcaattatag ggatttcatc cgcctgatac cagtcgaata
    31741 gcgctgccgc gcgctcagag tttattgttg acgaagaatt cccggtggca aattacgttg
    31801 atcagtttta tgtaaggtaa aaaatgttat acgcagttgc gcaaattatc cgcctttacg
    31861 tcactttatg agcaattcgc atataaaatg taaaactttt gtactagcat aaacacagaa
    31921 acgaatactg gcgacctggt cttgcggata aagcggtaat gagcaaacaa tcacagcatg
    31981 tattaattgc cctgccccac ccgctgcttc acctggtcag tttaggttta gtctcgttta
    32041 tctttaccct tttctcgctt gagctttcgc agtttggcac ccaactcgcc ccactgtggt
    32101 tcccgacgtc catcatgatg gtggcgtttt atcgccatgc cgggcgcatg tggccgggaa
    32161 ttgcgctgag ctgttcgctg ggaaatatcg ccgcatccat cctgcttttt tccaccagct
    32221 cgctgaacat gacctggacg accatcaata ttgttgaagc cgtggtcggg gcagtgctgc
    32281 tacgtaaatt gctgccgtgg tataacccct tgcaaaatct ggctgactgg ctgcgtctgg
    32341 cactcggcag cgccattgtt ccgcctctgt tggggggggg gtctggttgt cctgctgacg
    32401 gccggagacg atcctctcag ggcatttttg atatgggtac tgtcagaatc catcggcgct
    32461 ctggcactgg tgccgctggg attgttattt aaaccacact atctgctgcg ccatcgcaac
    32521 ccacggttgc tttttgagtc gctgctcacg ttagccatca cactgacgtt aagctggctt
    32581 tcgatgctgt atctgccgtg gccttttact ttcattattg tgctgttgat gtggagcgcc
    32641 gtgcgcctgc cacgaatgga agcctttttg atcttcctta ccacggtgat gatggtgtcg
    32701 ctgatgatgg ccgcggatcc ctccctgctt gctacgccgc gtacatacct gatgagccat
    32761 atgccgtggc taccgttttt gctgatcctg ctgcccgcca acatcatgac gatggtgatg
    32821 tatgcctttc gtgcggaacg caaacacatt tccgaaagcg aaacccgttt tcggaacgcc
    32881 atggaatatt ccgctatcgg tatggcgtta gtgggcaccg agggacaatg gctgcaaacc
    32941 aacaaagcgc tctgccagtt tctcgggtac agtcaggaag agctgcgcgg actcaccttt
    33001 cagcaactga cctggccgga ggatctcaat aaagatctcc aacaggttga aaagctgata
    33061 agcggtgaaa taaacaccta ttcaatggaa aaacgctact acaaccgcaa tggcgatgtt
    33121 gtctgggcgt tgcttgccgt ctcactggtg cgccacacgg atggcacgcc gctctatttt
    33181 atcgctcaga ttgaagacat taacgagcta aaacgcaccg aacaggtgaa tcagcaactg
    33241 atggagcgca tcacgctggc taacgaagcg ggcgggattg gcatctggga gtgggagttg
    33301 aagccgaata tttttagctg ggataagcgg atgttcgagc tgtatgaaat tcctccacat
    33361 atcaaaccga actggcaggt gtggtacgag tgcgtgctgc cggaagatcg ccagcatgcc
    33421 gaaaaagtga ttcgtgattc gttgcaatca cgctcgccct ttaaactgga atttcgcatt
    33481 accgtaaaag acggtattcg ccatatccgc gccctcgcca accgggtact gaataaagaa
    33541 ggcgaagtcg aacgcctgct cggcattaat atggatatga ctgaggttaa acagcttaac
    33601 gaggcattgt ttcaggaaaa agagcgcctg cacataacgc tggactccat cggcgaagcc
    33661 gtggtctgta ttgatatggc aatgaaaatt acctttatga atccagtggc ggagaagatg
    33721 agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt gcatattacc
    33781 tttggcgaca atggtcccct gatggagaac atttacagtg ccgacacctc acgttccgcg
    33841 attgaacaag atgtggtgtt gcactgccgg agcggcggca gctacgacgt gcattacagt
    33901 attacgccgt taagtactct ggacggcagc aatattggtt cggttctggt gattcaggac
    33961 gtcaccgaat cacgcaaaat gctacgccag ctgagctaca gcgcctccca tgatgcacta
    34021 acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct gcaaacggta
    34081 aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt taaagcggtg
    34141 aatgacagcg ccgggcacgc cgctggcgac gctttactgc gcgaactggc gtcgttgatg
    34201 ctgagcatgc tgcgctccag cgacgtgctg gcgcggctcg gcggtgatga atttggtctg
    34261 ctgctgccag actgcaatgt cgaaagtgcg cgttttatcg ctacacgtat tatcagtgct
    34321 gtgaatgact atcactttat ctgggaagga cgtgtacatc gggtaggtgc cagtgccggg
    34381 attaccttga ttgatgacaa caatcatcag gcggctgaag tgatgtcgca ggctgatatc
    34441 gcctgttatg cctccaaaaa tggtggccgt ggccgggtga cgatttacga accgcagcaa
    34501 gctgccgcac atagcgaacg gacggcgatg tcgcttgatg aacagtggcg gatgattaaa
    34561 gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc ggaagcgcgt
    34621 aatttgtggc tgatttcgct taaactctgg agttgcgaag gcgagattat tgatgaacaa
    34681 aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg acgggtattc
    34741 cacgaatttt tccagctggc cgcaaaagcg gttgccagta aaggcataag catcgccctc
    34801 cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct tgagcagctg
    34861 gaaaatagcc ctctaccacc acggttatta catctgatta ttccggcaga cgcgatttta
    34921 gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt gctcagccag
    34981 gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga ttacctgcta
    35041 cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat gctgattacg
    35101 attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc agtcgtttta
    35161 cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg tgatgtgatt
    35221 gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat taactgaggg
    35281 agcgtttgcc cattgcctga tgcgacgcat caggcctaca attcgtccgt tccgtctggt
    35341 tgccagcctt ccgtatacca gatatgcaac agcgcataag aacgccaggg cttccagcgc
    35401 tcggcataac ggcggatttg tgccggtgtc atccccggaa atcgctgttt aatcagataa
    35461 tcatccggca gaaaaacatc tttcgcctgc cagccacgca aagcgaaata attcgccgtc
    35521 cagcgcccga tacccggaaa agtttgcagc gttttcatcg cctgctccac atcgcccgga
    35581 attgtcattg gtaagctgcc ctccagcgcc gcatttgcca gatgaatcag tgcctctgcc
    35641 cgtttcaacg gcatgcctaa cgcttttaat gcctgcgggt cggctgctgc cagccgctga
    35701 ggcgtgggga agcagatata ctccggaaaa tcatccagcc gttcgccata aagctgtacc
    35761 actttggcag tcaattttgc cgccatcgcc acgctcacca gttggcctaa aatcgcccgc
    35821 acaccctgct caaaagcatc aacacagccg ggtaaacgca atccgggccg cgccgcgcct
    35881 aacttgccca acgcaccgtt aacaatctgc gggttacatt gcagatcaag caggcggctc
    35941 attttcgcca gacactcagc ggcaacaggt tctaaacctg cacttaaatt tatgtgcaga
    36001 gtatgacggg ccatatccgg aatagcagtc actacgccgc gatattcgcc caccgccaga
    36061 ctacgggcat aataatcgtc agcgaccgtt tccacgccac tcactgcacg ggcggcgaga
    36121 aatcccaaca tccacgacca gtcatacggc ggctgccagt tcagggtata catcgcatct
    36181 ccttattcat ccgctttcag cataaacgtt attcagacgc tacgctttgc tttcatattc
    36241 cggttgtcgc gacggcaaca tttcgctaaa gtcacgcccc ttcttcaccg gcatggggat
    36301 taattcgtgt ttattggttt tgattacggt acagcaaact gttcagtggc ggtcatgcgt
    36361 gacggtaaac cgcaattact gaaaatggaa aacgacagca cgctgctgcc ttcaatgctt
    36421 tgcgcgccaa cgcgtgaagc agtaagtgaa tggctgtacc gccatcatga tgttccggca
    36481 gacgacgatg aaacgcaggc gctgttacgt cgggcgattc gttataaccg cgaagaagat
    36541 atcgatgtta cggcgaaaag cgtgcagttc ggtctttcct cactggcaca gtacattgat
    36601 gatccagaag aagtgtggtt tgtgaaatca ccaaaatcgt tcctcggtgc cagcggctta
    36661 aaaccgcagc aggtagcgct gtttgaggat ctggtctgcg caatgatgtt gcacattcgc
    36721 cagcaggcgc aggcgcagct gccagaagcg attactcagg cggtgattgg tcgcccgatc
    36781 aacttccagg ggctgggcgg cgatgaagca aacgcccagg cgcaagggat tctggaacgc
    36841 gcggcgaagc gtgccggatt cagggacgtg gtattccagt acgagccggt cgcggctggg
    36901 ctggattacg aagccacctt gcaggaagaa aaacgggtgc tggtggtgga tatcggcggt
    36961 ggtacgactg actgttcatt gctgctgatg gggccgcagt ggcgttcgcg tctcgatcgt
    37021 gaagccagtc tgctgggtca cagtggttgt cgtattggcg gtaacgatct ggatatcgcc
    37081 ctggcgttta aaaacctgat gccactgctg ggtatgggtg gcgaaaccga aaaaggcatc
    37141 gccctgccga tcctgccgtg gtggaatgcg gttgccatca acgacgtacc tgcgcagagt
    37201 gatttctaca gtagtgccaa cggtcgtctg cttaacgatc tggtacgcga tgcccgcgaa
    37261 ccggaaaaag tggccctgtt acagaaagtc tggcgtcagc gtttaagcta tcgcctggtg
    37321 cgtagcgcag aagagtgcaa aattgctctt tcaagcgtag cggaaacccg cgcctcactg
    37381 ccgtttatca gcaatgaact ggctacgctg attagccagc gagggctgga aagcgccctc
    37441 agccagccac tggcgcggat tctggaacag gtgcaactgg ccctggataa cgcccaggaa
    37501 aaaccggacg ttatctacct gaccggcggt agcgcccgtt ctccgctgat taaaaaagcg
    37561 ctggcagaac agttgccggg cattccgatt gcaggcggcg atgactttgg ctccgtcacc
    37621 gccgggctgg cacgctgggc ggaagtggtg tttcgttaat tgaaaaatgg ctcagcagcg
    37681 gcttaaatct gcctgcgcct gagccagttt ttctgctgcc tgacgtaact gttccatcaa
    37741 cggcatggtc cgtgctggag catgaaggcg gaatcgctgc aactctgtcg cgccgactgt
    37801 caacgccgat tccagccgat gtcgtttggc ggtcattttc gccattaccg cttgtctgtc
    37861 tgctggcgtt atcgcttcat tcggcctgaa cacaaaacgg cgttcacagc tcgctttcca
    37921 gtcgataacc gcctgggtca gatggtcgcc aaaccctttc acttgcttaa cgccacgacg
    37981 ggtaacatct gcggctgttt caataccaaa agaccgtaac gccgctttac gcgcagggcc
    38041 aacgccggga atagaggcaa catcaataaa aaatccctcc agaaacttct gcttctgccg
    38101 ttcccttgca gtgtcatgaa gtgctgccag agcgcgtttc tcttcttccg gtaacccgag
    38161 aatttcgtcc ttcatttttt cgagcatcgt ccgtttggcg ataaaacctt ccaggccgcc
    38221 tacctgctgg atctggcgga ctaaatggtc atagtccatt ttcgcacgat taaatgcctc
    38281 acggcgttgc tgaacttctg ctttgtacgc tttgcttgtc agactgccaa taatccagat
    38341 agccgccagc acaggaacca aaataatata acgcggttct gcctgaaggc cgcaaagcag
    38401 cagcgataat gctgacagtg cgatctcaag cagaatgatg tattcgcgcc gtaacaggcc
    38461 taaaggaagc ggcctgccag tcggttggaa atgatcgggt aatggcagtt gcaatgctgg
    38521 cggtgctact gacgccatca ccatcgccca gacttttgcc agcacaaaat ccccgccggt
    38581 ggtaatgacc tcttcgccga gatcaataaa atagataaca ccttgattat ccagcgcaca
    38641 ccatgggcag tcagccaaat gaccgggata aacatgcatt gccgaaacgg tacatttctt
    38701 taactgttgg cgcagagaat caagtgccgc tacccacgct ttagccgtcg gacgcccggt
    38761 tgccacgcca ctttccgtga atgcctgctg aaacatggct tcaacatcac tcggtaacat
    38821 cgatagcgga atcgatcgtg gcggcggttt taaaccacgt cgctgattat ctgacgcgta
    38881 ggcataacgg aaatgggtaa tatccgtctc cagcgcatta cccgcatcag agataagcgg
    38941 cacaccagaa taaggatgcc ggccaccaaa caagacgtga aaaatcagca acgcaaggcc
    39001 aaaattatcg tgattttctg tgcgttcaaa gccgacaaat gacgacaagg tttgtagctc
    39061 tggcggcgta aaatgcgaca cgccgacttc gcataaatgc agtgtgccat tggcgttaat
    39121 ctgaaaggag tcgctatcaa tcagtaccac tttgctgtcg cgacctacca taaaactgtt
    39181 ctggtttacg tcacccacga cgtgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat
    39241 attgcgcgca acatagagta gaaaatccca cgcacaatga gggtaacgtt gacgacgatg
    39301 tgccgggcta tagatcatat gaatcggttc tttaccagaa acttttggca tcataaaacc
    39361 gataactttt ccgcctcgtc caccgtgaag cgttgcctgc ggccaggcga cataattcaa
    39421 caattgcgcg tcagctgtcg cagccataaa ggcaagtttg tcctgtttta aggcgggtgg
    39481 cggcgtgtga taaatcttgg cgacgctatc gacaaactca ttgatatcat aaaccgcgcc
    39541 ttcaccgcct ttgcccagtt cacggcctgg cgtaacgcac tcaccagtag cagtatataa
    39601 agtgggtttc atggtaacca cagcgccagt gctaacgttt tatcatcatc ggtacgctcg
    39661 tttaccgctg ggctggacaa aaactgcttt aacaattcag gtaataaatc gagttgttcc
    39721 tgcgttgctg acgccagtcc attgaaaaac ggggtaaaga aaggcacatg cggagaatta
    39781 tccaacatat ttaacgccag tcgctggata ccatccgtaa atgctgcaac tttatgcaca
    39841 cgctcagtgc tggtgaaagt ttccagtctg gaaacggcat cttcatcggt aatgaagtgc
    39901 gtcatattgg catattcacc caccatcggc gtaagcggca attgcagacc gtgaccaaaa
    39961 tccaccacca cgccgccatc gccaatctgc ataatcaagg tgccattagc cgatgaaatc
    40021 aatcccagaa acgtgcaggc aaaatcgcga accgctaatt ctttggcctc tgcctcggca
    40081 aaaagtcgct gacgaacggt cagcacaata tccgtggcga ggatatcatt aagccccaat
    40141 tccccaccct gcactttttg cgacatataa gccatcgctt cattgacggc gagcatcgcg
    40201 ccttcgccac cctgcgagac gctgcctgca ccatcagcaa gaaacatcac cagtaatggt
    40261 tgctgatcgt ttaaccaggc aacctgcatc tggcaggcat cctgacaagg taaatcagcg
    40321 ctaatgtgcg acgttccaac agcggatgca taaaccagac gccagctcac actgacgtcc
    40381 agccttttgg cgcttccagc acaacttccg ttcccggcgt ggagcgggaa accgaatgca
    40441 gcgaactgga taaccagcta aacagttcac ggaattgtaa tccttgcaga ggcaaaggct
    40501 gacgaacgct gatttgtgcc agcgtcttca tatccgcccc ctgaacagcg atggaaaaga
    40561 aggcaaactt cttatcttct tccccctgaa acactttgtt ggcggcagcc tgccactcat
    40621 cggttggagc gccatcggtg atcaggaaaa tccacgggcg ataatacgaa ataccatttg
    40681 cgcggtactc acgtttcctc tcttcaacca tatccagggc tttggtaatg gcggccccca
    40741 ttggcgtatc cccctgggca aacaggatgg gcgggaaaaa gttagcggcg ctggtaaagg
    40801 gttgttccac atgcaccggg ccgaacgtca caataccgag ttccactctt tttaaggcca
    40861 gcgaatcggc aagcagctca tcgcgaaagg taaccaatcc ggcgttaagt tcattaatgg
    40921 gtctgccgct catcgagcca gaaacatcca gcagcaaaat gcaggggcaa cgtggttccg
    40981 ggttactggc aaaatcgctg gtggcaaacg tgatttgttc gctcataaga aatcctttct
    41041 tagagaatta ttgaggtccg gtatcatacc gactgcaata atgaaataag agcaaacgaa
    41101 gaaactttca gaattaggcc cggcggcgca gaaaattttg ctattcgcct cgcaagaacg
    41161 gagagccgtc gattgacact aagggcggag tgacataatt tcaggagtga gggttaggga
    41221 gaggtttccc cctccccctg gtgttcttag taagcctgga agctaatcac taagagtatc
    41281 accagtatga tgacgtgctt catcataacc ctttccttat taaaagccct cttctccggg
    41341 agaggctttc ccgtttcagc gtcccgctga aatcatcggc ttacctcctt tcgccctgaa
    41401 tgcagtctat cgctaacgcg tgaataaaat agtttcctct gtgattattg cgtaacgact
    41461 cgcaaagaat atttgctggc aggatcgcag actacaaagc ctgcggattg acaatcttat
    41521 cgtgaaggca tactttcagg agtgagggta gagcggggtt tcccccgccc tggtagtctt
    41581 agtaagcggg gaagcttatg actaagagca ccacgatgat gagtagcttc atcatgaccc
    41641 tttccttatt taaggcccct tcctcgggag gggctttccc gtttcagcgt cccgctgaaa
    41701 tcgtcggctt acctcctttc gccatgcaag cagtctatcg ctaacgcgta gataaaatag
    41761 tttcctgtgt tattactgga tgcgtgctcg caaatgtgcc cgccattcag acgattcccg
    41821 atagtgtttc ataattcctc catttttctc ccttattggc tggctacact agtatcattc
    41881 cgcgaaacgt ttcaggaaga gaaactctta acgatgaaag gcagttataa atcccgttgg
    41941 gtaatcgtaa tcgtggtggt tatcgccgcc atcgccgcat tctggttctg gcaaggccgc
    42001 aatgactccc agagtgcagc cccaggggcg acgaaacaag cgcagcaatc gccagcgggt
    42061 ggtcgccgtg gtatgcgttc cggcccatta gccccggttc aggcggcgac cgccgtagaa
    42121 caggcagttc cgcgttacct caccgggctt ggcaccatta tcgccgctaa tactgttacg
    42181 gtgcgcagcc gcgtggacgg ccaactgata gcgttacatt tccaggaagg ccagcaggtc
    42241 aaagcaggcg atttactggc agaaattgac cccagccagt tcaaagttgc attagcacaa
    42301 gcccagggcc aactggcaaa agataaagcc acgcttgcca acgcccgccg tgacctggcg
    42361 cgttatcaac aactggcaaa aaccaatctc gtttcccgcc aggaactgga tgcccaacag
    42421 gcgctggtca gtgaaaccga aggcaccatt aaggctgatg aagcaagcgt cgccagcgcg
    42481 cagttgcaac tcgactggag ccgcatcacc gcaccagtcg atggtcgcgt tggtctcaag
    42541 caggttgatg ttggtaacca aatctccagt ggtgatacca ccggaattgt ggtgatcacc
    42601 cagacgcatc ctatcgattt agtctttacc ctgccggaaa gcgatatcgc tactgtagtg
    42661 caggcgcaaa aagtcggaaa accgctggtg gtagaagcct gggatcgaac caactcgaag
    42721 aaattaagtg aaggcacgct gttaagtctc gataaccaaa tcgatgccac taccggtacg
    42781 attaaagtga aagcacgctt taataatcag gatgatgcgc tgtttcccaa tcagtttgtt
    42841 aacgcgcgca tgttagtcga caccgaacaa aacgccgtgg tgatccccac cgccgccctg
    42901 caaatgggta atgaaggcca ttttgtctgg gtgctgaata gcgaaaacaa agtcagcaaa
    42961 catctggtga cgccgggcat tcaggacagt cagaaagtgg tgatccgtgc aggtatttct
    43021 gcgggcgatc gcgtggtgac agacggcatt gatcgcctga ccgaaggggc gaaagtggaa
    43081 gtagtggaag cccagagcgc cactactccg gaagagaaag ccaccagccg cgaatacgcg
    43141 aaaaaaggag cacgctcctg atgcaggtgt tacccccgag cagcacaggc ggcccgtcgc
    43201 gcctgtttat tatgcgtcct gtcgccacca cgctgctgat ggtggcgatc ttactcgccg
    43261 ggattatcgg ttatcgcgcc ctgcccgttt cggcgctgcc ggaagtggac tatccgacca
    43321 ttcaggtggt cacgctctac ccaggtgcca gcccggatgt catgacctct gccgttaccg
    43381 cgccgctaga acgccagttc gggcagatgt ctggcctgaa acagatgtcg tcgcaaagtt
    43441 ccggcggtgc gtcagttatc actttgcagt tccagctaac attaccgctc gatgtcgccg
    43501 agcaggaagt gcaggccgcg attaacgctg cgaccaactt gttgccgagc gatctgccta
    43561 acccgccggt ttacagcaaa gtgaacccgg cagatccgcc gatcatgacg ctcgccgtca
    43621 cctcaaccgc catgccgatg acgcaagtgg aagatatggt ggaaacccgc gtcgcgcaga
    43681 aaatctcgca gatttccggt gtcggactgg tgacgctttc cggcggtcag cgtccggctg
    43741 ttcgcgtcaa acttaacgct caggcgattg ccgccctcgg cctgaccagc gaaaccgtgc
    43801 gcaccgccat taccggcgct aacgttaact cggcaaaagg tagcctcgac ggcccttccc
    43861 gtgcggttac gctttccgct aacgaccaga tgcaatccgc cgaagagtat cgccagctaa
    43921 tcatcgccta ccagaacggc gcgccaattc gtctgggcga tgtcgcaact gtagagcaag
    43981 gtgcagaaaa cagctggctc ggcgcgtggg cgaacaaaga acaggccatt gtgatgaatg
    44041 ttcagcgcca gcccggtgct aacattatct ccaccgccga cagcattcgg cagatgctgc
    44101 cacagctcac tgagagtctg ccgaaatcgg tgaaggtgac agtgctttcc gatcgcacca
    44161 ccaatatccg cgcatccgtc gatgatactc agtttgaatt gatgatggct atcgcgctgg
    44221 tagtcatgat tatctacctg tttttgcgca atattccggc gaccatcatt cccggtgttg
    44281 ctgtaccgct gtcgttaatc ggcactttcg cggttatggt gtttctcgat ttttcaatca
    44341 ataacctgac actgatggcg ttaactatcg ccaccggatt cgtggtcgat gacgccatcg
    44401 tggtgatcga aaacatttcc cgctatatcg aaaaaggcga aaaaccgttg gcggcggcgc
    44461 tcaagggcgc aggtgaaatc ggctttacca ttatctcgct gaccttctca ctgattgcgg
    44521 tgttgatccc actgctgttt atgggcgata tcgtcgggcg actgttccgc gaatttgcta
    44581 ttaccctggc ggtagcgatt ttgatctcag cggtggtgtc gctgaccctg acaccgatga
    44641 tgtgcgcgcg gatgctcagc caggagtcgt tgcgtaaaca gaaccgcttc tcccgtgcct
    44701 cggaaaaaat gttcgacagg ataatcgccg cctatggtcg tggactggcg aaagtgctga
    44761 atcatccgtg gctgacctta agcgtggcac tcagcacgct gctgcttagc gtgctgctgt
    44821 gggtgttcat tccgaaaggt ttcttcccgg tacaggacaa tggcattatt cagggcactt
    44881 tgcaggcacc gcaatccagc tcctttgcca atatggccca gcgacaacgc caggtcgcgg
    44941 acgtgatttt gcaggatccg gcagtgcaaa gcctgacctc atttgttggc gttgatggca
    45001 ctaacccgtc gctgaacagt gcacatttac aaatcaacct caaaccgttg gatgaacgtg
    45061 atgatcgggt gcaaaaagtc atcgcccgtc tgcaaacggc ggtagataaa gtgccgggcg
    45121 tcgatctctt cctgcaacca acgcaggatc tgactattga tactcaggtc agccgcaccc
    45181 agtaccagtt taccttgcag gccacgtcac tggatgcgct cagtacctgg gtgccacagt
    45241 tgatggaaaa actccagcaa ctgccacagc tttctgatgt ctccagcgac tggcaggaca
    45301 aagggctggt ggcgtatgtc aatgttgatc gcgacagcgc cagccgtctg gggatcagca
    45361 tggcggatgt cgataacgcc ctgtacaacg cgtttggtca gcggctgatt tccactattt
    45421 atactcaggc caaccagtat cgcgtggtgc tggagcacaa caccgaaaat accccaggcc
    45481 tcgcggcgct ggataccatt cgcctgacca gcagcgacgg cggcgtggtg ccgctaagct
    45541 caattgccaa aattgagcag cgttttgcgc cgctctccat caaccatctg gatcagttcc
    45601 cggtaacgac catctccttt aacgtgccgg ataactattc gctgggcgat gcggtgcagg
    45661 cgattatgga caccgaaaag acgctgaatc tgccggtgga tatcaccacg cagttccagg
    45721 gcagcaccct cgccttccag tcggcgctgg gcagcactgt ctggctgatt gtcgcggcgg
    45781 tggtggcgat gtatatcgtg ctcggcattc tgtacgagag ctttattcac ccgatcacca
    45841 ttctctcgac gctacccacc gcaggagtcg gcgcactgct ggcgttaatg attgccggta
    45901 gcgaactgga tgtgattgcg attatcggca ttattttgct gatcggtatc gtgaagaaga
    45961 acgccatcat gatgatcgac ttcgcgctgg ctgctgagcg cgagcaaggc gtgtcgccgc
    46021 gcgatgccat ctaccaggct tgtctgttgc gttttcgtcc gatcctgatg accactctgg
    46081 cggctctgct tggcgcgctg ccgctgatgt tgagtaccgg ggtcggcgcg gaactgcgtc
    46141 gtccgttagg tatcggcatg gtcggcggcc tgattgtcag ccaggtgctg acgctgttta
    46201 ccacgccggt gatttatctg ctgttcgacc gcctggcatt gtggaccaaa agtcgctttg
    46261 ctcgccatga agaggaggcg taagtgaagt tttttgccct cttcatttac cgtccggtgg
    46321 cgacgatttt actgtcggtt gccattaccc tgtgcggcat actgggcttc cgtatgctgc
    46381 cggtcgcccc gctgccgcag gtcgattttc cggtgattat ggtcagcgcc tcgctgcccg
    46441 gtgcgtcacc agaaacaatg gcgtcttccg ttgccacgcc gctggagcgc tcacttgggc
    46501 gcattgccgg agtcagtgaa atgacctcca gcagttcgct cggcagcacg cgtattattt
    46561 tgcagtttga ttttgaccgg gatatcaacg gcgcggcgcg cgatgtgcag gcggcgatca
    46621 acgctgcaca aagtttgctg cccagcggga tgcccagccg tccgacctat cgcaaagcta
    46681 acccgtcgga tgcgccaatt atgatcctca cgctgacatc cgatacttat tcgcagggtg
    46741 aactgtacga tttcacctcg acgcagctgg ctccgacgat ttcgcaaatc gacggtgttg
    46801 gtgatgtcga tgtcggcggc agctcactgc ccgccgtacg cgtcgggctg aatccgcagg
    46861 cgctgtttaa tcagggcgtc tcgctggacg acgtacgcac tgccatcagc aatgccaacg
    46921 tgcgtaaacc gcagggggcg ctggaagatg gcactcaccg ctggcagatc cagaccaatg
    46981 atgagctaaa aaccgccgcc gaatatcagc cgctgattat tcattacaac aacggcggcg
    47041 cggttcgtct gggcgatgtg gcgacagtga ccgactctgt acaggatgta cgtaacgccg
    47101 ggatgaccaa tgccaaaccg gctattttgc tgatgatccg caagctgccg gaagccaata
    47161 ttatccagac ggttgacagc atccgggcaa aattaccgga gttgcaggaa accattccgg
    47221 cggcgattga tctgcaaatt gctcaggatc gctcccccac cattcgcgcc tcgctggaag
    47281 aagtcgagca aacgctgatt atctcggtgg cgctggtgat tctggtggtg tttttattcc
    47341 tgcgctcggg tcgcgcaact attattcccg ccgttgcggt gccggtttcg ctgattggta
    47401 cgtttgcggc gatgtacctg tgcggtttca gtctcaataa tctttcgtta atggcgctca
    47461 ccatcgctac tggtttcgtg gtggatgacg ccatcgtggt gctggaaaac attgcccgtc
    47521 atctggaagc gggaatgaaa ccgttgcaag ccgcactgca aggtactcgc gaagtcggtt
    47581 ttacggtgct gtcgatgagt ctgtcactgg tggcggtgtt cctgccgctg ctgttgatgg
    47641 gcggattgcc gggccgactg ttacgcgaat ttgccgtgac gctttctgtc gccattggta
    47701 tttcgttgct ggtttctctg acattaacgc caatgatgtg tggctggatg ctgaaagcca
    47761 gcaagccgcg cgagcaaaag cgactgcgtg gttttggtcg catgttggta gccctgcaac
    47821 aaggctacgg caagtcactg aaatgggtgc tcaatcatac ccgtctggtg ggcgtggtgc
    47881 tgcttggcac cattgcgctg aatatctggc tgtatatctc gatcccgaaa accttcttcc
    47941 cggagcagga cactggcgtg ttgatgggcg ggattcaggc ggatcagagt atttcgtttc
    48001 aggcgatgcg cggtaagttg caggatttca tgaaaattat ccgtgacgat ccggcagtgg
    48061 ataatgtcac cggctttaca ggcggttcgc gagtgaacag cgggatgatg tttatcaccc
    48121 tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt ctgcgcgtaa
    48181 aactggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaggat attcgcgttg
    48241 gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac ctggcggcac
    48301 tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa ctggcggacg
    48361 tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac cgcgacacca
    48421 tggcacggct gggaatcgac gtacaagccg ccaacagtct gttaaataac gccttcggtc
    48481 agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg atggaagtgg
    48541 atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc aataacgaag
    48601 gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc ccactatcgg
    48661 tgaatcatca gggattatcg gcggcctcga ccatttcgtt taacctgccg accggaaaat
    48721 cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggg gtgccttcga
    48781 cggtgcgcgg cagttttgcc ggcacggcgc aggtgttcca ggagacgatg aactcgcagg
    48841 tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc ctttacgaga
    48901 gttacgtaca tccgctgacg attctctcca ccctgccctc ggcgggcgtt ggagcgctgt
    48961 tggcgctgga gctgttcaat gccccgttca gcctaatcgc cctgataggg atcatgctat
    49021 taatcggcat cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt gaagcccaac
    49081 ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg cgttttcgcc
    49141 cgattatgat gactaccctg gcggcgctgt ttggtgcgct gccgctggta ttgtcgggcg
    49201 gcgacggctc ggagctgcgg caacccctgg ggatcaccat tgtcggcgga ctggtaatga
    49261 gccagctcct tacgctgtat accacgccgg tggtgtatct ctttttcgac cgtctgcggc
    49321 tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagat cttcccgaca
    49381 gcacccgctg gcaattgtgg attgtggctt tcggcttctt tatgcagtcg ctggacacca
    49441 ccatcgtaaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt ccgttgcata
    49501 tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc gccagcggct
    49561 ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg ctgtttactc
    49621 tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg gcacgcgcgt
    49681 tacagggcgt tggcggcgcg atgatggtgc cggtcggcag attgacggtg atgaaaatcg
    49741 taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt caggtcggtc
    49801 cgctgctcgg tccggcgctc ggcggtctgc tggtggagta cgcgtcgtgg cactggatct
    49861 ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgatg ttaatgccga
    49921 actataccat gcagacgcgg cgcttcgatc tctccggctt tttattgctg gcggttggca
    49981 tggcggtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg ccgctgacga
    50041 ttgctggcct ggtcgcagtt ggcgtggtag cactggtgct ttatttgctg cacgccagaa
    50101 ataacaaccg cgctcttttc agtctgaaac tgttccgcac tcgtaccttt tcgctgggcc
    50161 tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg acaccggttt
    50221 tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg atcccgatgg
    50281 tgcttggcag catgggaatg aagcgaattg ttgtacaggt ggtgaattgc tttggttatc
    50341 gtcgggtact ggtagcgacc acgctgggtc tgtcgctggt caccctgttg tttatgacta
    50401 ccgctctgct gggctggtac tacgttttgc cgttcgtcct gtttttacaa gggatggtca
    50461 actcgacgcg tttctcctcc atgaacaccc tgacgctgaa agatctcccg gacaatctgg
    50521 cgagcagcgg aaacagcctg ctgtcgatga ttatgcaatt gtcgatgagt atcggcgtca
    50581 ccatcgccgg gctgttgctg ggactttttg gttcgcagca tgtcagcgtc gacagcggca
    50641 ccacacaaac cgtctttatg tacacctggc ttagcatggc gttgatcatc gcccttccgg
    50701 cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt tcgcggcgaa
    50761 aaaggagcgc gcaatgaagt tctggcgacc cggtattacc ggcaaactgt ttctggcgat
    50821 tttcgccacc tgcattgtct tgctgatcag tatgcactgg gcggtgcgta tcagttttga
    50881 gcgtggcttt attgattaca tcaagcatgg taatgagcag cggctgcaaa tgctcggcga
    50941 tgccctgggc gagcagtatg cgcagcatgg caactggcgc ttcctgcgca acaatgatcg
    51001 ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata aacccgtccc
    51061 gggtatgcca ccgcacggct ggcgtaccca gttctgggtg gttgatcaaa acaacaaagt
    51121 gctggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca ttctggtcaa
    51181 cggtgcggaa gttggcgcgg tgatcgcctc ccccgttgag cggttaacgc gcaatactga
    51241 tatcaatttc gataaacaac agcggcaaac cagctggttg attgtcgctc tggcaacgtt
    51301 actcgcggcg ctcgccacct ttctgctggc gcgcggttta ctggcaccgg taaaacgact
    51361 tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa cgcccaccag
    51421 tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag cttgccagca cactggagaa
    51481 aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc gtacgccatt
    51541 agcggtgctg cgcggtgaac tggaagccat tcaggatggc gtgcgtaaat tcacgccgga
    51601 gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg acgatctcca
    51661 tcagttgtcg atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg tagatttgat
    51721 cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagtc gtggcctgaa
    51781 actgcaattt tccctgccag acagtattac cgtatttggc gatcgcgacc gtttaatgca
    51841 gttattcaat aacttactgg aaaacagcct gcgctacact gacagcggcg gcagcctgca
    51901 aatctctgcc gggcagcgcg acaaaacggt gcgcctgacc tttgccgaca gtgcgccagg
    51961 tgtcagtgac gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg aaggttcccg
    52021 caaccgtgcc agcggcggtt ccgggctggg gctggcgatt tgcctgaaca ttgttgaagc
    52081 acataatggt cgcattattg ctgcccattc gccttttggc ggggtaagca ttacagtaga
    52141 gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat cgacgaaaac
    52201 acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct cattgattat
    52261 ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt actgccgtat
    52321 gtgcgccaga caccaccgga tctgatcctg ttagatctga tgctccctgg caccgatggc
    52381 ctgacgctgt gccgggaaat tcgtcgtttt tctgacattc cgatcgtgat ggtgacggca
    52441 aaaatcgaag agatcgatcg cctgctgggg ctggagattg gcgcagatga ttatatctgt
    52501 aagccgtaca gcccacggga agtggtagcg cgcgtcaaaa ccattttgcg ccgttgcaaa
    52561 ccgcagcgcg aattgcagca acaggatgct gaaagcccgt tgattatcga cgaaggtcgt
    52621 tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt tcgtctgctg
    52681 aaaacgctat ctcatgaacc aggaaaagtg ttctcccgcg agcatttgct caatcatctt
    52741 tatgacgact accgcgtagt aaccgaccgc accatcgaca gccacattaa aaacctgcgc
    52801 cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta cggcgtcggt
    52861 taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatta ttttgttagc
    52921 cggagatggc gttcagaccc gatccggcaa cattatccca cgcatggtca gcaaactgac
    52981 attatctctc cgccctggca ggcatcagaa aatcgctcat actttaatcg gtaaacagca
    53041 cctttagatg ctgttttcga tacacaattt caatcaagga gtcattatgg ctggttggtt
    53101 tgaactcatt aagagcagtg ataatcagtt ccggtttgtg ctaaaagcgg gcaatggtga
    53161 gactatcctc accagcgagc tgtatacctc aaaagcctct gcggaaaagg gcatcgcgtc
    53221 ggtgcgtagc aacagcccgc aagaagaacg ctatgagaaa aaaacggcaa gtaacggcaa
    53281 attctatttc aatctgaaag ccgctaatca tcaaattatc ggctccagcc agatgtacgc
    53341 caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa gtcaatggca caagccagac
    53401 ggtgaaagac aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc ggctttatat
    53461 ccaaataacg cttagttatt aataattaag agtaaccgcc gccattaaaa tagtgggcac
    53521 tgacgagcac tcgttgtgct ttctttgttt accatcccat acttatcctc ctgaacatgt
    53581 tcatgtgcca aaatatcttc taatactttc gtgacgctat ccggtttatg acatacactg
    53641 atgtaagatt tttacttaca ggcgggcgta aaatggccag aacgatgacg gttgatctcg
    53701 gcgatgagtt gcgtgaattt atcgagtcct taattgaatc cggcgactat cgcacccaaa
    53761 gcgaagtgat ccgtgagtca ctgcgattac ttcgagaaaa gcaagcagag tctcgcctgc
    53821 aagcgttacg tgacatgctc gcagaagggt tgagtagtgg tgaagctcag ccgtgggaaa
    53881 aggacgcgtt cttacggaag gtgaaagcag ggataaggaa ataatgtgcg tattattaaa
    53941 ctcatgccaa aagccaatga ggatttagaa ggcatctggt attacagcta tcaccatttc
    54001 ggcgaaccac aagccgacag atatgtggaa catctttcag acgttctcca gattctgagt
    54061 aacaataata taggtacgcc cagacctgaa ttaggcgaag gtatttttgt tcttcctttt
    54121 gaacgccacg tcatttattt cctgcaatca ccaggggaaa ttatcgtcat tcgtatcctt
    54181 aaccaaaacc aggacgccac cagacattta cactggagct aaacccgccg attgcgctac
    54241 aatgcccgcc cttaaagtgg gggcactccc ctaaccgctt catcaggtga agcggatctg
    54301 acctgtcatc agaacgagag aattatgttt aaaccggaac tcctttcccc ggcgggaacg
    54361 ctgaaaaata tgcgttacgc tttcgcttat ggcgcagatg ctgtttatgc gggccagccg
    54421 cgttattccc tgcgtgtgcg caacaacgaa ttcaaccacg aaaatcttca gctcggcatc
    54481 aatgaagccc acgcgctggg gaaaaagttt tatgtcgtgg tcaacattgc accgcacaac
    54541 gccaagctga aaacctttat ccgtgacctg aaaccggtgg tggaaatggg gccggatgcg
    54601 ctgattatgt ccgatccagg gctgattatg ctggtgcgtg agcacttccc tgaaatgccg
    54661 atccaccttt cggtgcaggc taacgccgtg aactgggcga cggtgaaatt ctggcagcaa
    54721 atgggcctga cccgcgtgat cctctctcgc gagctgtcgc tggaagagat tgaagagatc
    54781 cgcaatcagg tgccggatat ggagatcgag atcttcgttc acggcgcgct gtgcatggcc
    54841 tactccggtc gctgcctgct ctctggctat atcaacaagc gcgacccgaa ccagggcacc
    54901 tgcaccaacg cctgccgctg ggagtacaac gtccaggaag ggaaagaaga tgatgttggc
    54961 aacatcgtac acaagtacga gccgattccg gtgcaaaatg ttgagccgac gctgggtatc
    55021 ggcgcaccaa ccgacaaagt gtttatgatc gaagaggccc agcgtccggg cgagtatatg
    55081 accgcgtttg aagatgagca cggcacttac atcatgaact cgaaagatct gcgcgccatc
    55141 gcccatgtag aacgcctgac caaaatgggc gtgcattcgc tgaaaatcga aggtcgtacc
    55201 aaatctttct actattgtgc acgcaccgca caggtttacc gcaaagctat cgatgacgcc
    55261 gctgcgggaa aaccgttcga taccagcctg ctggaaactc tggaaggtct ggcgcatcgt
    55321 ggctataccg aaggtttcct gcgtcgtcat actcacgacg attatcagaa ctacgaatac
    55381 ggttattcag tttctgaccg ccagcagttt gttggtgagt ttaccggtga gcgcaagggg
    55441 gacctcgcgg cggtagcggt gaaaaataaa ttctccgttg gcgacagcct tgagctgatg
    55501 acgccgcaag gcaacattaa ttttaccctt gagcacatgg aaaacgccaa aggcgaagct
    55561 atgccgatag caccaggcga tggttatact gtgtggctcc cggtcccgca ggatcttgag
    55621 ctcaattacg cgctgctgat gcgtaatttc tccggggaaa ccacgcgtaa tccccacggt
    55681 aagtgattaa tttcgattat ttttcccgga tggaaaattc ttagaaaccg atcacataca
    55741 gctgcattta ttaaggttat catccgtttc gctgaaaaac ataacccata aaatgctagc
    55801 tgtaccagga accacctcct tagcctgtgt aatctccctt acacgggctt attttttatg
    55861 cataagccct atccctggtc accgtcttcc attgaccaca tcgatagaat ctcccttcat
    55921 agcacgatgc ctttcacgta acggcatcgt gctcgcacag gttccggcta agcacaacca
    55981 gaacgcgcat gtttgacgct taccaaaaaa tattctcact ctccacattt gaatgtcaga
    56041 cgagcgacgc catgtaatcc tgcaccttct gtcttcaggt caactatctg catttttttg
    56101 ccctgagtaa cacagaaatg ggctgcatca ttttttacta tattttctgc accagatatt
    56161 ctgcccctgg ctaaagaagc ttcggcttcg gtgtagtatt ggttatcgag tttacgctga
    56221 atattacttt tatatgcaag accaaattta ccgatacttg tctcatcatt atgcacagca
    56281 caaccagaca caataaaaat actaattaat gatatagcag ctatcttttt catctcacct
    56341 tcccccatta aataccaacg acactctcta gtgtttaaat ataataatgg catgattatt
    56401 ataattgaat aggattataa taaatgttct gtacaacatt tcctacataa gtaggaatta
    56461 cggacattga ggcccttcag ggtaactcca tgggggcttt aatatattat attgaagatg
    56521 ccactgttta gttgaatatt aggtatatgc tcttttttga aatttatcgg tggcagccgt
    56581 tagtattcgc tgtccccatt gcaagctcct ggtggtaacc actgaatcct ccatacttga
    56641 actgactttt tatcctccga ctttcatcct gttctgactc caccttttgt tttctgctct
    56701 acactatcta cagaccaatc ataaaggcac atacgatcat ggcagaattt cccgccagct
    56761 tactgattct taatggcaaa agtactgaca atctaccctt gcgcgaagca attatgctgt
    56821 tgcgtgagga aggaatgacg atccatgtgc gggtcacctg ggagaaaggc gatgccgcac
    56881 gatatgtaga ggaggcccgg aagttgggcg tcgcaacggt gattgccggt ggtggcgatg
    56941 gcaccattaa tgaagtttct acggcgttga ttcagtgtga gggggatgac atacccgcgc
    57001 tgggaatttt gccattagga accgccaatg attttgccac cagtgtaggg attcctgagg
    57061 cactggataa ggcgctgaaa ctggcaattg ccggtaacgc cattgcgata gatatggcgc
    57121 aggtcaacaa acaaacctgt tttattaata tggcgacagg cggatttggg acgcgtatta
    57181 ccacagaaac gccggaaaaa ttaaaagccg cgctgggtgg cgtctcttac atcattcatg
    57241 gcttaatgcg catggatact ctgcaaccgg accgttgtga aatccgcggt gaaaactttc
    57301 actggcaagg tgacgccctg gtcattggta ttggtaacgg gcgtcaggcc ggtggcggtc
    57361 aacaattgtg cccgaacgcg ttaattaacg atggcttgct gcaactgcgc atttttaccg
    57421 gcgatgaaat tcttccggct ctcgtatcaa ccttaaaatc tgacgaagat aacccgaata
    57481 ttatcgaagg cgcttcgtcg tggtttgata tacaagcccc acacgaaatc acttttaatc
    57541 ttgatggcga accgttgagt gggcaaaact tccatattga aatacttccg gcggcgttgc
    57601 gttgtcgatt accaccggat tgtccattat tgcgttaatc aaactattct ctcttgaacc
    57661 agggagaatc cgaaatgccg gatgcggtgt aaatacctta tccggcatac agaacaatac
    57721 tacaccgtaa ttaattttac ccgtgtcttc tgcaatgcca gtttatccac ctctgataca
    57781 gcatcatcgg taataataat gtcaattttt tcgattggca gtacctgatt aaaaccgcga
    57841 cggttaaatt tcgatgaatc gagaacggca atcactttat tagcggcggt gaccatcgca
    57901 ccgctaatcg aataaccttc attaaaggtc gtaataccat taaccgcatc aataccatca
    57961 gcaccgacaa acattaaatc ggcattaata tcctgcaggg aacgttcagc aatagatcca
    58021 tgcatagatc gcgttttatg gcggaccgtg ccaccgcaga cgaccagagt aatgtcttta
    58081 ttttcggaaa gggcaaacgc agccgggaga ctgttggtaa tcaccgtgat atttttagcg
    58141 gtcattaatc cttcggcgat aagcattgtg gtacttccgc tgtcgagaat aacggtcatc
    58201 ccttcatgga tcattttgac tgccgcctgc gctattctgt ttttgggcgc gctggcgagc
    58261 tgaaatcgct ctttaaaccc gacttcctgg gtctcggttt cactattacc ggacattatt
    58321 ttcgccgcac cgccatgaaa gcgcgtaacc acgccttttt gttcgagaaa gcgcaaatcg
    58381 gcacggattg tcgcttccga ggcagcaaat actcccgcca gatcctgaac aagcacggtt
    58441 ccctgttcat tcactaattg gatgatctta ttccttcgct cgaatgaatt catcgtcatt
    58501 tacccatatt caattgtggc tagtgtaaac gaagggggac cattggtgaa tgatctgttt
    58561 tgcacaatac cgggccagca tcacgctggc ccgcggtttc atggaatgag caacactttg
    58621 cccggcatgg cattacgagc gatgtcacgc accgcctggg tgaagctttc aaagcttcca
    58681 cggtgagcga ttaatggctc caggcttaac ttacgttctg tcagcaaccg gctcgccgtt
    58741 tcccactcct gccccggcca agggctggaa tagttcatcc aactgccgat aaccgtcagc
    58801 tctttacgca atattttgcc aaacgttgcc gatgttaaat gcagatcctg atgcaacgtg
    58861 cccaccagcg ccagttgggc atgaggaccg gcaatctcta ccgccagttc gacagtttgc
    58921 ggtacgccag ccgtctcgag gataagctga ttaaaacgca gttcgcgtaa aacgctctgc
    58981 atttgcggcg cgctcatttc gctactgtta aatgtttgca tcgcaccgaa agattttgcc
    59041 agtgccagtt tttctgagct aatgtcgata gccgtcacac tctttgctcc cagcgcgaca
    59101 gcgcactgaa tagccagcag gccaatggtt ccggcaccaa taataataac gtttttattc
    59161 tcacaacctt gcgctaaatg aaaagcatgc agaccaacgg taatcggctc aataaaagcc
    59221 ccatcctcaa taggcatatc cgtgggtaga gcaaagacat tttttcgctt aacgacaata
    59281 tattcagcaa atccaccatc acgccgcgag ccaataaaat catattttgc gcactgggaa
    59341 taaaaccctt tcagacactc tggacaagta aaacagggta ataacggcac acaggcaacc
    59401 gcatcgccag ggtgtaaatc atcaacaccg gatcccaccg catcaatata gccgctaaat
    59461 tcatggccta acgttattgg ataataatgt gcaccatttt taaatatcct gggtaaatcg
    59521 gaaccacata agcccgagct ggcaattttt acccgcacct catcctgatg tttaatttca
    59581 ggaatgacgc tttctgcaac gcgcacgata ccatcagtat cattcaccac tgatttcata
    59641 aaaactcctg attgttaagg gggataacct ccccctcagg taaaaattat tctgcgagaa
    59701 cgactttctc ttgtttaata aagccacgcg ctctacgcca ggtcatgaaa ataccggtca
    59761 gataaattgc accgataata atgaaaccgg gaatattttg cggggagaaa acctgaatca
    59821 gtaaccaggt aatgggagaa ccgccctgat ccattgaagc caccataccc ccggctttta
    59881 acgccccagc attagccgcc agttgggtgt gcaaaccaat agtttgcgtc gcgatccaca
    59941 gggtgatgct cataatgatg acacccgaga ttaaggtgcg gaacagattt ccacgatgca
    60001 ccgcgaccgc catcgccaca aagaagccga tagtggcaag atcgccaaac ggcagcacct
    60061 gattacccgg cacacaaaca gcaattaaaa tggtgagtgg gataaaaatc aggcttgccg
    60121 ataccaccgc cgtatgcccc agcagtaatg cgggatccag gccaatcagg aactcctgac
    60181 cgccgaactt cgcctgtaaa cgactacggg cctgcttagc gatgggcgtt aaaccatcca
    60241 tgatgggttt aatcacccgt ggcatcagca gcatcactgc cgccgttttt accgccagct
    60301 gcaatacacc tttgacatcg taacccgcga ggatgccgat aatcagcccc atcacaaaac
    60361 ccacggtgac aggttcgcca aaagggccaa atttgcgctg aatatcgtcg gcgctaaatt
    60421 taattcggtt aacgcctggg attttctcga tgatagcatc gaccagcacc tcaatcggcc
    60481 ccatatacgc cgacgtaccg tgcggaatag caataccttc cagctcaaag aaatttcggg
    60541 tatcgcgggc aaaccagtcg ccgagcttat aaacaaacgc cgcgtgaatt accacacctg
    60601 ccatccctat catccatgaa ccggttgcca ggtgcagcaa cgcgccggtg aaggtcatat
    60661 gccagatatt ccagatatca acatttacca cccgcgtcat acgggtcagt aacatcgcca
    60721 cgttaaccaa tatcgcaatc ggaatcgcca ccagcgcaat ttgcgacgcc caggtcattg
    60781 gtgaagagcc cggccagccg acatcgacca catgcagatt caggtcgaaa ttttccgcca
    60841 tcgctttcgc cgccggacca atggaatcca gcattaagcc aatcacaagg ccaatgccaa
    60901 caaagccaat cccgatatgc agacccgctt taaagcaatc gcctgccttc atgcctaata
    60961 ttttagaaaa aataatgatg acaaccggca gcatcactgt agggccgagg tcgagaatat
    61021 aacgcatgac ttctgaaaac ataggtcacc cctgtaagat agtcagaatt ttattttgta
    61081 atgcttcgat accgacacca gaaataaaag gcatgccgtg aactaacgga atatcgccaa
    61141 aactacgatc cactttggca gtggtgcata tcaaatgcac accatccata taggtttcta
    61201 tttcattaac ccgacactgg attaattcaa caggaatatt atgattctga cacaactctt
    61261 taatttcttc cgccgccatc gtagaggtcg caaccgcgcc tccgcaagcg acaataatct
    61321 tgcgtttcat aaatagtcct ttttattgtt acagagggat ttattcgtct gaatctaaaa
    61381 catactttgt gaagtattcc tttaactggg tttcaggaag agtgattagt gtctcgacga
    61441 tatcgggctg ttgtaacttg ccaaataaac agcgtaaaag tttcaattgc tgctgcggat
    61501 tttccacaat caacgcaata accaacgata cctccacgtc gttatcatca tccgcttgct
    61561 gaaaatgaac tttatttgtt ggccttaaca gataaatggc tgacgactta gcatgaatcg
    61621 cctcacaatg cggtattgca atggcgtgct gctcaagcat tatcccggta gggaattctg
    61681 cttctctggc aattaatgcc tgtggccagg tatcatgaac cacacctttg gcgagcatct
    61741 cattaccgat atgggttaaa acttcgctac gatcgacaaa agaaattccg ctacgaacaa
    61801 acaggttagt catataccgt ccttattccg cacagccgta gcgataggcg cgcaaaacat
    61861 cataaatttt atccataatc agctgatgcg gtattgctga taattccccg gactgaatgc
    61921 gttcaaattg tttgggaaga tactgactaa tcatgcccag tggggtgtcc acgccttcaa
    61981 gattcaccat catcgtttcg acgctatttt taatccgact atgcggccag taataacgaa
    62041 tacgatccga caggctgtaa cgaatatcca gtaatgaatc gttaaaaccc gtacgataat
    62101 attttttcca gtattgcggt tcgtcgagca tcacttcttc aattaccgcc aggcaaccgc
    62161 tgcgattttc gggggcgata agttcctgct caatttgtgc cagtgcaaat atcgcctcgc
    62221 gtaaagcaaa ggttaatgcg ggaccgactt tcaatattgc aaagtgatcg cggactaatt
    62281 cccaataagc cgtccgggtc tggtaatcgg tagaatgtgc ttcataaacc attcgggtgt
    62341 tttctatcca ttgcgccagc gcctgcgctt cctgcggctg ataatggata atattgctgt
    62401 gatcaaattc cacacccggc tgcaccacga tggcaatcac acgtgttaac gcctctgtca
    62461 gcccacgggc aataaaggcc ttttgatgcg tacgtaaagt attggcggca tcttcaacat
    62521 gggtgatgtg tactgactga atggcgctgg cctcaccgcc cggaaccgga acttcggtgc
    62581 caatgacata gctcagttgc tcacgctggc aatctgtcgc cacactttcc gcagcaaagc
    62641 acagcagctc gttccgcaac cgtttctggt gctaacggta tgggatcccc cgcgcaggac
    62701 attgacgcat caagatgaat tttactgaag ccagcacgaa catatgcttt taccagctcg
    62761 acggattttt ccatcgccgc atccgcattt tcttgctgcc agcagtttgg ccccagatga
    62821 tcgccgccga gaataatgcg ttcgcgtgca aacccaactt tatcggcaat cgtaaaaaca
    62881 aattcgcgaa agtctgccgg tgtcattccg gtataaccgc caaattgatt gacctggttt
    62941 gacgttgctt caatcagcac tttgcgcgtg ctgttgcgat caaatgccag cgccgcttcg
    63001 ataaccaacg gatgggcaga acagactgaa catatgccga tatgttcacc agctttatgc
    63061 cgggcaatta acgttttcat gttttttcct tgttaaatgg caggtgcgtt atgccctgcc
    63121 ctcgcagcca caatcggcaa tcactttgct caccacatcg cgcattgcgg atttagccga
    63181 ctgcaaataa tcccggggat cggtcgcttc agggtgctcg gtcaggtaat tttttaacgc
    63241 ctgcgagaag gcatttttca gctccgttgc aacgttgatt ttgcatatcc ccagtttgat
    63301 ggtttgctga atatccttag tcgataaccc tgacgcgcca tgcagcacca gcggtaagtt
    63361 cacccactgg cgaatgttct ccagtcgaga aaaatcaagc gccggtgcgc tggcatacat
    63421 cccatgagcc gtgccgatgg cgaccgccag ggaatcaatt ccggttgcct cggcaaattc
    63481 acgcgcctga gcggggttgg tataaaaagc atcggcttca ttgacttgca catcatcttc
    63541 ctggccgcca agttgcccca gctccgcttc gacgctgaca tcaaagcgat ggcaaaaatc
    63601 caccacctct ttgacccgtg aaatattttg cgcaaaaggc aaatgcgagg cgtcaatcat
    63661 gactgagcgc acgccagaac gaaccttctg agcgatatcg tcaaatttcg tgtgatggtc
    63721 gagatgaatt gccagtggat ggtgatattg cttcgccatc gcgctgacca gcgccaacag
    63781 attttctgta ccagcatgag taaatgtgcc aggcgttccg gcgatgatga ccggcgcatg
    63841 caggttggca gcggtttcca ccaccacttg catcgtttcg agattgtgaa tattgaatgc
    63901 cggaaccgca taaccgccgc gctgtgcgtt gttcagcatc tgctttgtcg ataccacgta
    63961 cattttcata tcctgtcgtt tgttttcgat ttcaaaatat aatgaaatga tttgttttta
    64021 aatgtcgaga taacgatcac aaaaacgaca atgtgaaaat tgttcgagga gtaaaaggta
    64081 aaaaaaacgg cctcccgata gggaagccgt agcaaagtgc gcgtgttttt atgccggatg
    64141 cggtgtaaac atacgtggca gtgctatgta ggcatgataa gacgcttccg cgtcgcatca
    64201 ggcataggtt gccggatgca gtgtaaacgc cttatccgtc ctacggggtg gtgctgttcg
    64261 tatgaggcgt atcaggcaat agtaattttg ctatcgagat aaacgtcctg cacggcatta
    64321 atcagtttca cgccgtcagc catcgatttc ttgaacgcct tacgtccaag aatcagcccc
    64381 attccgccag cgcgtttgtt gataaccgca gtacgcactg catcgctgag gtcagtttca
    64441 ccgcccgcag caccgccgga gtttatcaac ccagcccgac ccatatagca gttagctaac
    64501 tgataacgca ccagatcaat cgggttttcg ctggtcaatt tgctgtaaac acgatcgtcg
    64561 gtgtaaccgt aattaattgc tttatagccg ccgttatttt ccgccatttt ttgtttgacg
    64621 atatctgcac cgatggttgc cgccagatgg tttgcctgac cggtcaggtc ggcggaaaca
    64681 tggtaatcaa cgccatcttt cttaaaggcg gagttacgca aataggccca cagcactgtc
    64741 accataccca gctcgtgcgc acgttcaaaa gccgcagaaa tttcttcaat ctggcgacgt
    64801 gactcttccg agcccaaata gatagtcgca ccaaccgcaa ccgcgcccat gttgaacgcc
    64861 tgctccacgc tggcatacag cgtttgatcg taggtattcg ggtaacttag cgtctcgttg
    64921 tgattaagtt tgacgaggaa tggaatgcga tgcgcataac gccgcgatac cgacgccagc
    64981 acgccgtaag ttgacgccac acagttacag cccgcttcga tcgccagttc aacaatgttt
    65041 ttcgggtcaa agtagagcgg gttagcagca aatgaagctc cggcagagtg ctcaacgccc
    65101 tggtcaaccg gcagaataga aagatatcct gtgccagcca gacgcccggt gttgtacaac
    65161 gtctgcatat tacgtaacac cgctggcggg cgattattgt caatcattac gcggtctacg
    65221 tagtcatgtc cggggagata aagctggtca gaaggaattg tcatacaacg gtgctgtaaa
    65281 aggttgtcgg cgtctttgcc aagcaactgc gcaatatctg tcattactat gctcccgtaa
    65341 attccgattg gatatcggct atggattgtc ctggcccgcc ttttgcgggc aatcataatc
    65401 ctggtcgtta cgggcgagtt tttccatctt ttggatgttt tttcagcgtt ttctgttggc
    65461 tcgattcatc agaaaaaatg ttagcgcggt caagttttca ccgcaaaggt atttaaaagg
    65521 tattattaag tggtattgtc atcgcgtacc ttacattacc tgtcatgaag gaattaaaag
    65581 atgaaaacaa cagcaaagct gtcgttcatg atgtttgttg aatggtttat ctggggcgcg
    65641 tggtttgtgc cattgtggtt gtggttaagt aaaagcggtt ttagtgccgg agaaattggc
    65701 tggtcgtatg cctgcacagc cattgcggcg atcctgtcgc caattctggt tggctccatc
    65761 actgaccgct ttttctcggc gcaaaaagtg ctggcggtat tgatgttcgc aggcgcgctg
    65821 ctgatgtatt tcgctgcgca acagaccact tttgccgggt tcttcccgtt actgctggcc
    65881 tactcgctaa cctatatgcc gaccattgcg ctgactaaca gtatcgcttt tgccaacgtg
    65941 ccggatgtgg agcgtgattt cccgcgcatt cgtgtgatgg gcactatcgg ctggattgcc
    66001 tctggtctgg catgtggttt cttgccgcaa atgctggggt atgccgatat ctcaccgact
    66061 aacatcccgc tgctgattac cgccggaagt tctgctctgc tcggtgtgtt tgcgtttttc
    66121 ctgcccgaca cgccgccaaa aagcaccggc aaaatggaca ttaaagtcat gctcggcctg
    66181 gatgcgctga tcctgctgcg cgataaaaac ttcctcgtct ttttcttctg ttcattcctg
    66241 tttgcgatgc cactggcgtt ctattacatc tttgccaacg gttatctgac cgaagttggc
    66301 atgaaaaacg ccaccggctg gatgacgctc ggccagttct ctgaaatatt ctttatgctg
    66361 gcattgccgt ttttcactaa acgctttggt atcaaaaagg tattattgct tggtctggtc
    66421 accgctgcga tccgctatgg cttctttatt tacggtagtg cggatgaata tttcacctac
    66481 gcgttactgt tcctcggtat tttgcttcac ggcgtaagtt acgattttta ctacgttacc
    66541 gcttacatct atgtcgataa aaaagccccc gtgcatatgc gtaccgccgc gcaggggctg
    66601 atcacgctct gctgtcaggg cttcggcagt ttgctcggct atcgtcttgg cggtgtgatg
    66661 atggaaaaga tgttcgctta tccggaaccg gtaaacgggc tgactttcaa ctggtccggg
    66721 atgtggactt tcggcgcggt gatgattgcc attatcgccg tgctgttcat gatttttttc
    66781 cgcgaatccg acaacgaaat tacggctatc aaggtcgatg atcgcgatat tgcgttgaca
    66841 caaggggaag ttaaatgaaa acagaacgta ttctcggtgc tctttatggg caggcgttag
    66901 gggatgcgat ggggatgccc tccgagcttt ggccacgcag ccgcgttaaa gcacactttg
    66961 gctggattga ccgttttctg cctggaccaa aggagaataa cgcggcctgt tattttaacc
    67021 gcgccgaatt caccgacgat acctcgatgg cgctgtgtct ggcggatgcg ttactggaac
    67081 gtgaaggcaa gatcgatccg gatctgattg ggcgtaatat tctcgactgg gcgctgcgtt
    67141 tcgacgcctt taacaaaaac gtactaggtc cgacctcgaa gattgcgctt aacgccattc
    67201 gcgacggtaa acccgttgct gaactggaaa acaacggcgt gaccaacggc gcagcgatgc
    67261 gcgtctcgcc attaggttgt ttgcttccgg cgcgtgatgt tgattccttt attgatgatg
    67321 tggcgctggc gtcgagcccg acccataaat ccgatctggc agttgcaggt gcggtagtca
    67381 tcgcatgggc gatttctcgt gccattgacg gagaaagctg gtcagcgatt gttgattccc
    67441 tgccttcaat tgcgcgacat gcacagcaaa aacgcatcac taccttcagc gcctcactgg
    67501 cagcacgtct ggagattgcg ctgaaaattg tgcgcaatgc cgacggcacc gaatccgcca
    67561 gcgaacagct ttaccaggtc gttggcgcag gtaccagcac tattgagtcc gttccgtgcg
    67621 ccattgcgct ggttgaactg gcacaaaccg acccgaatcg ctgcgccgtc ctgtgcgcta
    67681 accttggcgg cgacacagac accatcggcg ctatggcgac ggcgatttgc ggcgcgttgc
    67741 atggcgttaa cgctatcgat cctgcattaa aggcggaact ggatgcggta aatcagcttg
    67801 atttcaaccg ctatgccaca gcgctggcga aatatcgtca acaacgggag gcggtatgag
    67861 cggcgctcga ttacacacgc tgctgcctga attaacctcg cgtcagccgg tgatggtcgt
    67921 cggcgcggcg gtcattgatg tgatcgccga cgcttatgcc ctcccctggc gtgggtgcga
    67981 tatcgaactg aagcagcaga gcgttaacgt tggcggctgc gcactgaata ttgccgtggc
    68041 gttaaagcgc ctcggcatcg aagcgggtaa tgccttgccg ctcggtcagg gcgtgtgggc
    68101 ggagattatt cgcaaccgga tggcgaaaga ggggttaatc agcctgatcg ataacgccga
    68161 aggcgataac ggctggtgtc tggcgctggt tgagccggat ggcgaacgca cttttatgtc
    68221 attcagcggt gtggaaaatc agtggaatcg ccagtggctg gcgcgattaa ccgttgcgcc
    68281 tggcagcctg ctctattttt ccggttatca actggcctcg ccctgcggcg aattgttagt
    68341 ggaatggctg gaaaagctgc aagacgtgac gccgtttatc gattttggcc cacgtatcgg
    68401 cgatatcccg gacgcattac tggcgcggat catggcctgt cgacctttag tatcactcaa
    68461 tcgtcaagag gctgagattg ccgccgaacg ttttgcttta tccgcagaga taacaacact
    68521 tggcaagcaa tggcaggaga aatttgccgc gccgttgatc gttcgtctcg ataaagaggg
    68581 tgcatggtat ttcagcaacg acgcttctgg ctgcattccg gcatttccaa cgcaagttgt
    68641 agacaccatt ggggcgggcg acagtcatgc aggtggcgta cttgccgggc tggcctctgg
    68701 tctgccactg gcggatgccg tattactggg caatgcagtg gcgtcgtggg ttgtcgggca
    68761 tcggggcggt gattgtgcgc caacgcgcga ggaactactc ctcgcacaca aaaacgtata
    68821 gatcgctgcg acagtggcta atgctgtact caataggccg ttgctgttgg tcaagcgcca
    68881 cttgcttgat caccagcacc ggtattttgc tatctaactg aatatgtgac tgaaactcgg
    68941 catccggcat ccgggcgcta acgcgggaac gcgtacgttg tgggtaaatg tgctggctgc
    69001 ggaaatagtc ataaagtgaa atcccgatgg catcaacatc atgaattaaa tgcgccggaa
    69061 cccacgattc ctcaatcgat accgcttctt catcgacata gcgaatacgc ttaagcaaaa
    69121 agacatcact tcctgcttcg accgccagct gctgcgcgac ttcttcggga catttcacga
    69181 cacgcttgtt aacccatagc gtatcgggct ttttcccgcg caataccacc tgctgagaaa
    69241 aacctcgcgc ttctttcaac gagtattcga agatgttgtt gatctgcgtg ccgtaaccgc
    69301 gcgaacgcgt caccacaccc tcttcttcca gcgcctgcat cgccttgcgc accgtaatgc
    69361 gcgaaacgcc ggttaactga cttaaatcac gctcaccggg caaaatattg ccatgctcca
    69421 gcaccccgct gcgcacggca ttttttaccg tttcggcaaa cttaatataa agcggcgtgt
    69481 tatccgccgc taaaatacgc tcgttcagtt gggcaatcag ctgggtatgc gcttgttcca
    69541 tttgtctttt tcctgccgag gagtctggtg ggagtatatc actgcttctt atttaaggcg
    69601 tttctttaat cccatcaacg aatgcctgca actcctccac cgtgccatga aatacattaa
    69661 aatccaccgg accattgatg ccatctacct gtccacggtc ggaatgctgc cagaagcgcc
    69721 aggccatgcc gtcattgtcc ggacgacgtt gataatagtg agccacccac cacggatatt
    69781 cattgaaata gcccgccaga ttggtgtgat aaaaaacggc tcctgagtaa ataatcggct
    69841 tttttcccgt acttttttcg accattttta gccactgact tactcgtttg cgtaattctt
    69901 ttgccgataa ttttccccgt tcttctacgt ccagtaccgc agggaaatcg ccttgtaaga
    69961 aatccaccgt ttgcagaaat aatcgtgcct gaaccggcgc tgctaccgac ggggagaaat
    70021 aatgatacgc cccgcgcagc aggccatttt cgcggcttag ttgccagtta cgcgaaaaat
    70081 acgggtccac cagcttttcg ccttccgtcg ccttaataaa agcaaactgt aagcggatac
    70141 cgttgtcgcg catttttgcc acccgctgcc agtcgatccg ctcctgccag cgagaaacat
    70201 ctattccgtg aatggtgtaa ctggcaggaa tgcgaatagc gaaagattta accgggcgat
    70261 aaccgtagaa atggatgtag tctgtaatct gaactgctgt cgaatagaaa aaattcaccg
    70321 tctgacgcgg ataaattgca acgatggaaa ttagcccaag agcgcataat agagaggtga
    70381 gtttttttcg actggtaatt cttagctgca ttggtttcat ccctgaatgt cagtgccaaa
    70441 ggctgacaat aaccaaagca gctatagtac ggtgctcacc accaggcgtg gaagtggtga
    70501 accggaccaa taccgtgacc aacttccagc gtgtcggcct gggctaatgc tgatgaaagc
    70561 cagctttttg cctcctgtac ggtgtcagcc cagtttgtat ggcgcgggcg tagtgcagcc
    70621 aacgccgcag agagcgtaca accagtgccg tgggtgtttt tggtcataat gcgcggtgcg
    70681 gtaaaccgtt gttcaccctc gcgggtaaac agccagtccg ggctttgctc atcatccaga
    70741 tgaccacctt tcattagcac tgcgccacag cccatcgcca acagcgatcg cccttgttcc
    70801 agcatttcct gttcggtgcg cgcgtgtggc gcgtcgagca aggcggcagc ttcgggcaag
    70861 tttggcgtta ttaatgaaac ctgtggcaat aatcgactgc gcagcgtagc aaccgccgaa
    70921 ggtgaaagca gcgggtcgcc gctttttgcc agcataacgg tgtcgagtac cacgttttgg
    70981 atctgataac gttgcaaccg ttctgccacc gcttcaacaa tatcggtttc cgccaacata
    71041 ccgattttag tggtgtcgat tcgcacatcg ctgaacaccg aatcgagctg ggcggcgaca
    71101 aaatcaggct caatgcgata caccgactgt acgccacggg tattttgcgc caccagtgca
    71161 gtaataactg agcaaccata agcgccaagt gccgagaagg ttttaagatc ggcctgaatc
    71221 cccgcaccac cactcggatc agtaccggca atcgtcagag cgttaattcg tttcatgcct
    71281 gcacctcctg cgtcaattgc cagagcgcat caaggaaatg tggaacaaaa ctgcctggcc
    71341 cctcgcttct ggcgactgcg cgctctccgg cttgtttcat ccagtgacag gcagatgcga
    71401 cattttccag catatcgcct ggtaaggcac aacaggcagc aacaaccgcc gataatgcac
    71461 agccagttcc taccactttg gtcattagcg gatcgccacc gtgaatgcca acgatacgat
    71521 gcccatcggt aacataatcc acctcgcctg tgaccacgac gattgcgcca gtttcccgtg
    71581 ccagtgtttg tgcagcgggt atcgcgttag ctgcggcgtc agtggtatcc actccccgtc
    71641 cgccattagc aacgccagct aatgccatga tttccgaagc attaccacgt atcgctgtcg
    71701 gtttaaaaga taaaaattca tgacaaaaat ggcggcgata atcgagcgca ccaaccgcta
    71761 ctggatcaag cgtccagggt gtttgagagc tttttgcttg ctcaacggca gcacgcatcg
    71821 cctgtgcgcg tggctgcgtc agtgtgccaa cgttaatcaa caaggcactg gcgatagccg
    71881 caaactgact ggcctcttcg gtttcgataa ccatcgctgg cgatgcaccg agcgccagca
    71941 aggtattggc agtaaaggtt tgcaccacat cgttggtcat gcagtgcaca agaggggaat
    72001 gttggtgaaa aaggtgtaac gcgtgcgcag attgcgctga actcagcagg tcgacttgca
    72061 tagtttgctc ctgccataac gtgaagaagc aatgacctgg tggtccgtga cttccctacg
    72121 ctggcattat ccagatcagg tgatacgggt atttctcagc cttcacgcag aagggcaccc
    72181 cgagtcgttt ggttgcgatg ataaatcgca gaggaggatg gtaatgtcca gcgcacgcgt
    72241 tgtaaacgag gaaaaacggc agttttacaa tcgcgttaat aaattatttg atatatgaat
    72301 ccagcacctt cagaacgaca tccagatctt cttcacgttt tagctcatcc ccctggtgaa
    72361 cgatgtgttc cgtcagatga cctttaatca cttcccgcat cagaccgttt accgcgccac
    72421 ggatagcagc aatctgttgt aaaactgcag cgcattcgtg cggctcgtcg agcattttct
    72481 tgagcgccac gacctggccc tgaatcttac tggcacgcgc tttcagtttc tgtttatcac
    72541 ggattgtatg agacatggca acacctggtt aacaagaata tgaaaaatca tagcactatt
    72601 aatctactgg ggggtagtat caggtactgg gggggagtag
//
LOCUS       DAZGAN020000012        70839 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGAN020000012 DAZGAN020000000
VERSION     DAZGAN020000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 70839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.012"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(63..839)
                     /gene="sseB"
                     /locus_tag="ACHKYO_RS04540"
                     /old_locus_tag="pgaptmp_000908"
     CDS             complement(63..839)
                     /gene="sseB"
                     /locus_tag="ACHKYO_RS04540"
                     /old_locus_tag="pgaptmp_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HGU4427799.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKSVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(981..2264)
                     /gene="pepB"
                     /locus_tag="ACHKYO_RS04545"
                     /old_locus_tag="pgaptmp_000909"
     CDS             complement(981..2264)
                     /gene="pepB"
                     /locus_tag="ACHKYO_RS04545"
                     /old_locus_tag="pgaptmp_000909"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HGU4427800.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2442..2642)
                     /gene="iscX"
                     /locus_tag="ACHKYO_RS04550"
                     /old_locus_tag="pgaptmp_000910"
     CDS             complement(2442..2642)
                     /gene="iscX"
                     /locus_tag="ACHKYO_RS04550"
                     /old_locus_tag="pgaptmp_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HGU4427801.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(2654..2989)
                     /gene="fdx"
                     /locus_tag="ACHKYO_RS04555"
                     /old_locus_tag="pgaptmp_000911"
     CDS             complement(2654..2989)
                     /gene="fdx"
                     /locus_tag="ACHKYO_RS04555"
                     /old_locus_tag="pgaptmp_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HGU4427802.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(2991..4841)
                     /gene="hscA"
                     /locus_tag="ACHKYO_RS04560"
                     /old_locus_tag="pgaptmp_000912"
     CDS             complement(2991..4841)
                     /gene="hscA"
                     /locus_tag="ACHKYO_RS04560"
                     /old_locus_tag="pgaptmp_000912"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HGU4427803.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(4858..5373)
                     /gene="hscB"
                     /locus_tag="ACHKYO_RS04565"
                     /old_locus_tag="pgaptmp_000913"
     CDS             complement(4858..5373)
                     /gene="hscB"
                     /locus_tag="ACHKYO_RS04565"
                     /old_locus_tag="pgaptmp_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HGU4427804.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5469..5792)
                     /gene="iscA"
                     /locus_tag="ACHKYO_RS04570"
                     /old_locus_tag="pgaptmp_000914"
     CDS             complement(5469..5792)
                     /gene="iscA"
                     /locus_tag="ACHKYO_RS04570"
                     /old_locus_tag="pgaptmp_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HGU4427805.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(5809..6195)
                     /gene="iscU"
                     /locus_tag="ACHKYO_RS04575"
                     /old_locus_tag="pgaptmp_000915"
     CDS             complement(5809..6195)
                     /gene="iscU"
                     /locus_tag="ACHKYO_RS04575"
                     /old_locus_tag="pgaptmp_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HGU4427806.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6223..7437)
                     /gene="iscS"
                     /locus_tag="ACHKYO_RS04580"
                     /old_locus_tag="pgaptmp_000916"
     CDS             complement(6223..7437)
                     /gene="iscS"
                     /locus_tag="ACHKYO_RS04580"
                     /old_locus_tag="pgaptmp_000916"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGU4427807.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7549..8037)
                     /gene="iscR"
                     /locus_tag="ACHKYO_RS04585"
                     /old_locus_tag="pgaptmp_000917"
     CDS             complement(7549..8037)
                     /gene="iscR"
                     /locus_tag="ACHKYO_RS04585"
                     /old_locus_tag="pgaptmp_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HGU4427808.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(8338..9075)
                     /gene="trmJ"
                     /locus_tag="ACHKYO_RS04590"
                     /old_locus_tag="pgaptmp_000918"
     CDS             complement(8338..9075)
                     /gene="trmJ"
                     /locus_tag="ACHKYO_RS04590"
                     /old_locus_tag="pgaptmp_000918"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HGU4427809.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            9194..9997
                     /gene="suhB"
                     /locus_tag="ACHKYO_RS04595"
                     /old_locus_tag="pgaptmp_000919"
     CDS             9194..9997
                     /gene="suhB"
                     /locus_tag="ACHKYO_RS04595"
                     /old_locus_tag="pgaptmp_000919"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HGU4427810.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            10142..10996
                     /gene="yfhR"
                     /locus_tag="ACHKYO_RS04600"
                     /old_locus_tag="pgaptmp_000920"
     CDS             10142..10996
                     /gene="yfhR"
                     /locus_tag="ACHKYO_RS04600"
                     /old_locus_tag="pgaptmp_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4427811.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            11187..12467
                     /gene="csiE"
                     /locus_tag="ACHKYO_RS04605"
                     /old_locus_tag="pgaptmp_000921"
     CDS             11187..12467
                     /gene="csiE"
                     /locus_tag="ACHKYO_RS04605"
                     /old_locus_tag="pgaptmp_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HGU4427812.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12459..13598)
                     /gene="hcaT"
                     /locus_tag="ACHKYO_RS04610"
                     /old_locus_tag="pgaptmp_000922"
     CDS             complement(12459..13598)
                     /gene="hcaT"
                     /locus_tag="ACHKYO_RS04610"
                     /old_locus_tag="pgaptmp_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HGU4427813.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(13758..14648)
                     /gene="hcaR"
                     /locus_tag="ACHKYO_RS04615"
                     /old_locus_tag="pgaptmp_000923"
     CDS             complement(13758..14648)
                     /gene="hcaR"
                     /locus_tag="ACHKYO_RS04615"
                     /old_locus_tag="pgaptmp_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HGU4427814.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            14784..16145
                     /gene="hcaE"
                     /locus_tag="ACHKYO_RS04620"
                     /old_locus_tag="pgaptmp_000924"
     CDS             14784..16145
                     /gene="hcaE"
                     /locus_tag="ACHKYO_RS04620"
                     /old_locus_tag="pgaptmp_000924"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HGU4427815.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16142..16660
                     /gene="hcaF"
                     /locus_tag="ACHKYO_RS04625"
                     /old_locus_tag="pgaptmp_000925"
     CDS             16142..16660
                     /gene="hcaF"
                     /locus_tag="ACHKYO_RS04625"
                     /old_locus_tag="pgaptmp_000925"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HGU4427816.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            16660..16980
                     /gene="hcaC"
                     /locus_tag="ACHKYO_RS04630"
                     /old_locus_tag="pgaptmp_000926"
     CDS             16660..16980
                     /gene="hcaC"
                     /locus_tag="ACHKYO_RS04630"
                     /old_locus_tag="pgaptmp_000926"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HGU4427817.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            16977..17789
                     /gene="hcaB"
                     /locus_tag="ACHKYO_RS04635"
                     /old_locus_tag="pgaptmp_000927"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             16977..17789
                     /gene="hcaB"
                     /locus_tag="ACHKYO_RS04635"
                     /old_locus_tag="pgaptmp_000927"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HGU4427818.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            17799..19001
                     /gene="hcaD"
                     /locus_tag="ACHKYO_RS04640"
                     /old_locus_tag="pgaptmp_000928"
     CDS             17799..19001
                     /gene="hcaD"
                     /locus_tag="ACHKYO_RS04640"
                     /old_locus_tag="pgaptmp_000928"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HGU4427819.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            19098..19520
                     /gene="yphA"
                     /locus_tag="ACHKYO_RS04645"
                     /old_locus_tag="pgaptmp_000929"
     CDS             19098..19520
                     /gene="yphA"
                     /locus_tag="ACHKYO_RS04645"
                     /old_locus_tag="pgaptmp_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU4427820.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19568..20440)
                     /gene="yphB"
                     /locus_tag="ACHKYO_RS04650"
                     /old_locus_tag="pgaptmp_000930"
     CDS             complement(19568..20440)
                     /gene="yphB"
                     /locus_tag="ACHKYO_RS04650"
                     /old_locus_tag="pgaptmp_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HGU4427821.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20452..21488)
                     /gene="yphC"
                     /locus_tag="ACHKYO_RS04655"
                     /old_locus_tag="pgaptmp_000931"
                     /pseudo
     CDS             complement(20452..21488)
                     /gene="yphC"
                     /locus_tag="ACHKYO_RS04655"
                     /old_locus_tag="pgaptmp_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(21521..22519)
                     /gene="yphD"
                     /locus_tag="ACHKYO_RS04660"
                     /old_locus_tag="pgaptmp_000932"
     CDS             complement(21521..22519)
                     /gene="yphD"
                     /locus_tag="ACHKYO_RS04660"
                     /old_locus_tag="pgaptmp_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4427822.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22544..24055)
                     /gene="yphE"
                     /locus_tag="ACHKYO_RS04665"
                     /old_locus_tag="pgaptmp_000933"
     CDS             complement(22544..24055)
                     /gene="yphE"
                     /locus_tag="ACHKYO_RS04665"
                     /old_locus_tag="pgaptmp_000933"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU4427823.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQTLGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24078..25061)
                     /gene="yphF"
                     /locus_tag="ACHKYO_RS04670"
                     /old_locus_tag="pgaptmp_000934"
     CDS             complement(24078..25061)
                     /gene="yphF"
                     /locus_tag="ACHKYO_RS04670"
                     /old_locus_tag="pgaptmp_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU4427824.1"
                     /translation="MPKKMRTTRNLLLMGTLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLDAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(25158..28439)
                     /gene="yphG"
                     /locus_tag="ACHKYO_RS04675"
                     /old_locus_tag="pgaptmp_000935"
     CDS             complement(25158..28439)
                     /gene="yphG"
                     /locus_tag="ACHKYO_RS04675"
                     /old_locus_tag="pgaptmp_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HGU4427825.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVVLDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            28557..29750
                     /gene="yphH"
                     /locus_tag="ACHKYO_RS04680"
                     /old_locus_tag="pgaptmp_000936"
     CDS             28557..29750
                     /gene="yphH"
                     /locus_tag="ACHKYO_RS04680"
                     /old_locus_tag="pgaptmp_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGU4427826.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPKGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(29814..31067)
                     /gene="glyA"
                     /locus_tag="ACHKYO_RS04685"
                     /old_locus_tag="pgaptmp_000937"
     CDS             complement(29814..31067)
                     /gene="glyA"
                     /locus_tag="ACHKYO_RS04685"
                     /old_locus_tag="pgaptmp_000937"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGU4427827.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            31395..32585
                     /gene="hmpA"
                     /locus_tag="ACHKYO_RS04690"
                     /old_locus_tag="pgaptmp_000938"
     CDS             31395..32585
                     /gene="hmpA"
                     /locus_tag="ACHKYO_RS04690"
                     /old_locus_tag="pgaptmp_000938"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HGU4427828.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(32630..32968)
                     /gene="glnB"
                     /locus_tag="ACHKYO_RS04695"
                     /old_locus_tag="pgaptmp_000939"
     CDS             complement(32630..32968)
                     /gene="glnB"
                     /locus_tag="ACHKYO_RS04695"
                     /old_locus_tag="pgaptmp_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HGU4427829.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(33029..34363)
                     /gene="glrR"
                     /locus_tag="ACHKYO_RS04700"
                     /old_locus_tag="pgaptmp_000940"
     CDS             complement(33029..34363)
                     /gene="glrR"
                     /locus_tag="ACHKYO_RS04700"
                     /old_locus_tag="pgaptmp_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HGU4427830.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(34353..35066)
                     /gene="qseG"
                     /locus_tag="ACHKYO_RS04705"
                     /old_locus_tag="pgaptmp_000941"
     CDS             complement(34353..35066)
                     /gene="qseG"
                     /locus_tag="ACHKYO_RS04705"
                     /old_locus_tag="pgaptmp_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HGU4427831.1"
                     /translation="MRHIFQRLMPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(35231..36658)
                     /gene="qseE"
                     /locus_tag="ACHKYO_RS04710"
                     /old_locus_tag="pgaptmp_000942"
     CDS             complement(35231..36658)
                     /gene="qseE"
                     /locus_tag="ACHKYO_RS04710"
                     /old_locus_tag="pgaptmp_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HGU4427832.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(37216..41103)
                     /gene="purL"
                     /locus_tag="ACHKYO_RS04715"
                     /old_locus_tag="pgaptmp_000943"
                     /gene_synonym="purI"
     CDS             complement(37216..41103)
                     /gene="purL"
                     /locus_tag="ACHKYO_RS04715"
                     /old_locus_tag="pgaptmp_000943"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGU4427833.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKVLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            41361..42917
                     /gene="mltF"
                     /locus_tag="ACHKYO_RS04720"
                     /old_locus_tag="pgaptmp_000944"
     CDS             41361..42917
                     /gene="mltF"
                     /locus_tag="ACHKYO_RS04720"
                     /old_locus_tag="pgaptmp_000944"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HGU4427834.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIANSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(42914..43417)
                     /gene="tadA"
                     /locus_tag="ACHKYO_RS04725"
                     /old_locus_tag="pgaptmp_000945"
     CDS             complement(42914..43417)
                     /gene="tadA"
                     /locus_tag="ACHKYO_RS04725"
                     /old_locus_tag="pgaptmp_000945"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGU4427835.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(43475..44110)
                     /gene="yfhb"
                     /locus_tag="ACHKYO_RS04730"
                     /old_locus_tag="pgaptmp_000946"
     CDS             complement(43475..44110)
                     /gene="yfhb"
                     /locus_tag="ACHKYO_RS04730"
                     /old_locus_tag="pgaptmp_000946"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HGU4427836.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            44319..45167
                     /gene="yfhH"
                     /locus_tag="ACHKYO_RS04735"
                     /old_locus_tag="pgaptmp_000947"
     CDS             44319..45167
                     /gene="yfhH"
                     /locus_tag="ACHKYO_RS04735"
                     /old_locus_tag="pgaptmp_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGU4427837.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            45223..45483
                     /gene="yfhL"
                     /locus_tag="ACHKYO_RS04740"
                     /old_locus_tag="pgaptmp_000948"
     CDS             45223..45483
                     /gene="yfhL"
                     /locus_tag="ACHKYO_RS04740"
                     /old_locus_tag="pgaptmp_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HGU4427838.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(45677..45757)
                     /gene="shoB"
                     /locus_tag="ACHKYO_RS04745"
                     /old_locus_tag="pgaptmp_000949"
     CDS             complement(45677..45757)
                     /gene="shoB"
                     /locus_tag="ACHKYO_RS04745"
                     /old_locus_tag="pgaptmp_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HGU4427839.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(46177..46557)
                     /gene="acpS"
                     /locus_tag="ACHKYO_RS04750"
                     /old_locus_tag="pgaptmp_000950"
     CDS             complement(46177..46557)
                     /gene="acpS"
                     /locus_tag="ACHKYO_RS04750"
                     /old_locus_tag="pgaptmp_000950"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGU4427840.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(46557..47288)
                     /gene="pdxJ"
                     /locus_tag="ACHKYO_RS04755"
                     /old_locus_tag="pgaptmp_000951"
     CDS             complement(46557..47288)
                     /gene="pdxJ"
                     /locus_tag="ACHKYO_RS04755"
                     /old_locus_tag="pgaptmp_000951"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HGU4427841.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(47300..48028)
                     /gene="recO"
                     /locus_tag="ACHKYO_RS04760"
                     /old_locus_tag="pgaptmp_000952"
     CDS             complement(47300..48028)
                     /gene="recO"
                     /locus_tag="ACHKYO_RS04760"
                     /old_locus_tag="pgaptmp_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGU4427842.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(48040..48945)
                     /gene="era"
                     /locus_tag="ACHKYO_RS04765"
                     /old_locus_tag="pgaptmp_000953"
     CDS             complement(48040..48945)
                     /gene="era"
                     /locus_tag="ACHKYO_RS04765"
                     /old_locus_tag="pgaptmp_000953"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGU4427843.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTGGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(48942..49622)
                     /gene="rnc"
                     /locus_tag="ACHKYO_RS04770"
                     /old_locus_tag="pgaptmp_000954"
     CDS             complement(48942..49622)
                     /gene="rnc"
                     /locus_tag="ACHKYO_RS04770"
                     /old_locus_tag="pgaptmp_000954"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGU4427844.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(49895..50869)
                     /gene="lepB"
                     /locus_tag="ACHKYO_RS04775"
                     /old_locus_tag="pgaptmp_000955"
     CDS             complement(49895..50869)
                     /gene="lepB"
                     /locus_tag="ACHKYO_RS04775"
                     /old_locus_tag="pgaptmp_000955"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGU4427845.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(50885..52684)
                     /gene="lepA"
                     /locus_tag="ACHKYO_RS04780"
                     /old_locus_tag="pgaptmp_000956"
     CDS             complement(50885..52684)
                     /gene="lepA"
                     /locus_tag="ACHKYO_RS04780"
                     /old_locus_tag="pgaptmp_000956"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGU4427846.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(52882..53361)
                     /gene="rseC"
                     /locus_tag="ACHKYO_RS04785"
                     /old_locus_tag="pgaptmp_000957"
     CDS             complement(52882..53361)
                     /gene="rseC"
                     /locus_tag="ACHKYO_RS04785"
                     /old_locus_tag="pgaptmp_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HGU4427847.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(53358..54314)
                     /gene="rseB"
                     /locus_tag="ACHKYO_RS04790"
                     /old_locus_tag="pgaptmp_000958"
     CDS             complement(53358..54314)
                     /gene="rseB"
                     /locus_tag="ACHKYO_RS04790"
                     /old_locus_tag="pgaptmp_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HGU4427848.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKHLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(54314..54964)
                     /gene="rseA"
                     /locus_tag="ACHKYO_RS04795"
                     /old_locus_tag="pgaptmp_000959"
     CDS             complement(54314..54964)
                     /gene="rseA"
                     /locus_tag="ACHKYO_RS04795"
                     /old_locus_tag="pgaptmp_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HGU4427849.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(54997..55572)
                     /gene="rpoE"
                     /locus_tag="ACHKYO_RS04800"
                     /old_locus_tag="pgaptmp_000960"
     CDS             complement(54997..55572)
                     /gene="rpoE"
                     /locus_tag="ACHKYO_RS04800"
                     /old_locus_tag="pgaptmp_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HGU4427850.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(55569..55724)
                     /gene="rseD"
                     /locus_tag="ACHKYO_RS04805"
                     /old_locus_tag="pgaptmp_000961"
     CDS             complement(55569..55724)
                     /gene="rseD"
                     /locus_tag="ACHKYO_RS04805"
                     /old_locus_tag="pgaptmp_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HGU4427851.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            55980..57602
                     /gene="nadB"
                     /locus_tag="ACHKYO_RS04810"
                     /old_locus_tag="pgaptmp_000962"
     CDS             55980..57602
                     /gene="nadB"
                     /locus_tag="ACHKYO_RS04810"
                     /old_locus_tag="pgaptmp_000962"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HGU4427852.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(57587..58324)
                     /gene="trmN"
                     /locus_tag="ACHKYO_RS04815"
                     /old_locus_tag="pgaptmp_000963"
     CDS             complement(57587..58324)
                     /gene="trmN"
                     /locus_tag="ACHKYO_RS04815"
                     /old_locus_tag="pgaptmp_000963"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HGU4427853.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            58456..59790
                     /gene="srmB"
                     /locus_tag="ACHKYO_RS04820"
                     /old_locus_tag="pgaptmp_000964"
     CDS             58456..59790
                     /gene="srmB"
                     /locus_tag="ACHKYO_RS04820"
                     /old_locus_tag="pgaptmp_000964"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HGU4427854.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(59999..60880)
                     /gene="yfiE"
                     /locus_tag="ACHKYO_RS04825"
                     /old_locus_tag="pgaptmp_000965"
     CDS             complement(59999..60880)
                     /gene="yfiE"
                     /locus_tag="ACHKYO_RS04825"
                     /old_locus_tag="pgaptmp_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4427855.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            60983..61570
                     /gene="eamB"
                     /locus_tag="ACHKYO_RS04830"
                     /old_locus_tag="pgaptmp_000966"
     CDS             60983..61570
                     /gene="eamB"
                     /locus_tag="ACHKYO_RS04830"
                     /old_locus_tag="pgaptmp_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HGU4427856.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(61626..62009)
                     /gene="grcA"
                     /locus_tag="ACHKYO_RS04835"
                     /old_locus_tag="pgaptmp_000967"
     CDS             complement(61626..62009)
                     /gene="grcA"
                     /locus_tag="ACHKYO_RS04835"
                     /old_locus_tag="pgaptmp_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HGU4427857.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            62314..63003
                     /gene="ung"
                     /locus_tag="ACHKYO_RS04840"
                     /old_locus_tag="pgaptmp_000968"
     CDS             62314..63003
                     /gene="ung"
                     /locus_tag="ACHKYO_RS04840"
                     /old_locus_tag="pgaptmp_000968"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGU4427858.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(63051..64088)
                     /gene="yfiF"
                     /locus_tag="ACHKYO_RS04845"
                     /old_locus_tag="pgaptmp_000969"
     CDS             complement(63051..64088)
                     /gene="yfiF"
                     /locus_tag="ACHKYO_RS04845"
                     /old_locus_tag="pgaptmp_000969"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU4427859.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            64295..64714
                     /gene="trxC"
                     /locus_tag="ACHKYO_RS04850"
                     /old_locus_tag="pgaptmp_000970"
     CDS             64295..64714
                     /gene="trxC"
                     /locus_tag="ACHKYO_RS04850"
                     /old_locus_tag="pgaptmp_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HGU4427860.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            64783..65481
                     /gene="tapT"
                     /locus_tag="ACHKYO_RS04855"
                     /old_locus_tag="pgaptmp_000971"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             64783..65481
                     /gene="tapT"
                     /locus_tag="ACHKYO_RS04855"
                     /old_locus_tag="pgaptmp_000971"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HGU4427861.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            65513..68173
                     /gene="pat"
                     /locus_tag="ACHKYO_RS04860"
                     /old_locus_tag="pgaptmp_000972"
     CDS             65513..68173
                     /gene="pat"
                     /locus_tag="ACHKYO_RS04860"
                     /old_locus_tag="pgaptmp_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HGU4427862.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSHNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQELIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            68287..69642
                     /gene="pssA"
                     /locus_tag="ACHKYO_RS04865"
                     /old_locus_tag="pgaptmp_000973"
     CDS             68287..69642
                     /gene="pssA"
                     /locus_tag="ACHKYO_RS04865"
                     /old_locus_tag="pgaptmp_000973"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HGU4427863.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            69688..70011
                     /gene="yfiM"
                     /locus_tag="ACHKYO_RS04870"
                     /old_locus_tag="pgaptmp_000974"
     CDS             69688..70011
                     /gene="yfiM"
                     /locus_tag="ACHKYO_RS04870"
                     /old_locus_tag="pgaptmp_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HGU4427864.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(70008..>70839)
                     /gene="kgtP"
                     /locus_tag="ACHKYO_RS04875"
                     /old_locus_tag="pgaptmp_000975"
     CDS             complement(70008..>70839)
                     /gene="kgtP"
                     /locus_tag="ACHKYO_RS04875"
                     /old_locus_tag="pgaptmp_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HGU4427865.1"
                     /translation="RKGFYASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIP
                     FALGAVLAVVALWLRRQLDETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLC
                     FYTFTTYMQKYLVNTAGMHANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFG
                     SLAAIFTVPILSALQNVSSPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGV
                     GLSYAVANAIFGGSAEYVALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL
                     "
BASE COUNT        16866 a        19258 c        17856 g        16859 t
ORIGIN      
        1 ggtggttaac aaccttcaga gcagcaagta agcccgaatg ccgccctttg gggcggcata
       61 ttttagatta tccgattctg tttaaagtca cgcaaaaaac caccccagcg acgttcatag
      121 aatggcacaa tatgttcggt aataaagtgg ctaattcctt tttccccttt tttcacctga
      181 caaatatcga ttggttcatc gccaggtaat gtatcggtcg ctacacttcc cgtcgcctga
      241 ataatttctt cgatatcacc atcggcttca atgccaataa gtaaattagg ctgtgcctct
      301 tcgttctctt taattgaaca aataaaagca cgcttcaccg acttaatggt tttaaataag
      361 gtggtgagtg aatcaatcat ttgtgctggc ggctctgcga cttccgataa tatcagcgat
      421 tcaccgcctt ccaggatttc ctggctgctc agcggatttc cctcttcacc aatcaacaaa
      481 ctgatttcac gcggcataaa ttctttaccg gtcggcagtt tggcattaag gaagagcgtt
      541 tcgccaaggg tcatctcaaa caatgtgcga acgggcatta cgacaaatgc ctgttcgtct
      601 tcaaccgcct gttgaagcgc ttctaacgag gtgaaaaaag gaatgacgct ggtgccgtct
      661 tctttttccc agtgctgtaa atcaagcgca ctatcttcaa ccacagcctc gccctgcgcc
      721 gccgtaccag gcacccagac ggtggattcc agtagagtac ggaaaaaggc cgggcggtgc
      781 gccggttcgg ttgctgcttt ttccagcagg tcttcaagtt cgttttttgt ttcggacata
      841 agaaccacaa ttcattcaac gttcggggcg caaatgcctg atgcgctacg cttatcaggc
      901 ctacaaggga ttcgcaattt gttgaatttg cagaatttgt aggccggata aggcgtttac
      961 gccgcatccg gcatattagt ttacgccgtt aacagattag caatcgtgcg cacaccaagt
     1021 cccgtagcgc ccgcagacca ctgttcaacc ggcgctttac ggtaagtcgc cgagcagtcg
     1081 atatgcagcc agccttgctg atagttctca acaaagtgcg acaggaagcc cgccgccgtg
     1141 ctcgcgcctg ccggatacgc cgcgcttccg gtattgttca gttcggcaaa gttagacggc
     1201 agctggctgc ggtggaattc cgccagcggc agacgccaga acggttcgtt ctcttgtgag
     1261 gcactcgcca gcaaacgacc ggcaagcgca tcgtcaaaac tgaacagcgc gtgataatca
     1321 ttacccagcg cagttttcgc cgccccggtg agggtcgccg catcaatgat cagttccggt
     1381 ttctgcgcac tggcatcaat cagaccatcg gccagcacca gacgcccttc ggcatcagtg
     1441 ttcatcactt caactttttt accgttgcga taggtgatga tatcgcccag cttgaacgca
     1501 ttgccgctaa tcaggttatc cgcacagcag aggaacagct tcacgcgctt gttcagtccg
     1561 cgcgtaatgg caaatgccag cgccccggta accgttgccg cgccgcccat gtccgacttc
     1621 atcgagtcca taaacgcagt ctgttttatg ctgtagccgc cggagtcaaa agtgatacct
     1681 ttacctacca ggcacgcgta caccggtgct tctttatcgc cagttgggtt gtaatccagc
     1741 gccagcaata ccggagaacg ttctgaaccg cgcccgacag tgtgcagccc catataacct
     1801 tgctcacgca gatcttcgcc tttggtgatc cgataagtca cacgatcgcc cgcgacgttg
     1861 ctgatcagat caacagcacg ctgtgccagt tgcgatggtc ccaattcttc tgccggtgcg
     1921 ttgatggtgt cacgcaccca gtcgatgatc atcaggcggt tatccagttc ctggcgctgg
     1981 gcatcgtcca gatccggcca ctccacttta cgcgtgcctt tcggggcttt gtaaccttgc
     2041 cagaatgccc agcagcgatc cgcatcccag ccttcaccgc ttaactgaac atgcttgatg
     2101 cccagaccgt caatcttgcg cgccgcacgc tggatcagcc ccagatcgtc tgccccgttc
     2161 aaatgcaggg taatgccgtc attattaatg ctgtaagttg ctttttctcc ccagcgcgcg
     2221 tcggcaggtt gggtagagag ggtaatcttc atcgcttctg tcattttagt tatccttctt
     2281 tgtaaaaggg ccgccagttg gcagccccag gatttttccg ctaaatgatt cgcgttgcag
     2341 gaaggcggca agtgagtgaa gccccaggag catagataac tatgtgactg gggtgaacga
     2401 gcgcagccaa cgcatctgcg gcgtgaagca tgacgcggaa attattcggc ctcgtccagc
     2461 cagactaaca aaatcgcttc gaggattttc tcgttggatg cctgcgggtc gtcgtcgaaa
     2521 tcttccaaat cgcaaatcca ctgatgcata tcggtgaatc gaaccgtttt cggatcaaga
     2581 tcgggatacg catcgtacag tgcttcgcca atttcgcggc tatcggtcca cttaagtccc
     2641 atactaacct ctgttaatgc tcacgcgcat ggttgatagt gtaacgcggg atttcgacca
     2701 ctaaatcttc gtcggtgacg cgcgcctggc agcttaaacg gctttccggc tccagtcccc
     2761 aggctttgtc cagcatgtcg tcttcctgct ctgagctttc cggcagcgag tcaaaacctt
     2821 cacgaacgat gcagtggcag gtggtgcaag cacaggattt ttcacaggcg tgttcaatct
     2881 cgataccgtt acgcagagct gcgtcgagaa tggtttcacc gctattagct tccagaacag
     2941 cgccatcagg gcagagatcc tgatgaggca aaataacaat ctttggcata ttaaacctcg
     3001 tccacggaat ggcctttcag cgcacgacga accgactggt ccatgcggcg agcggcgaaa
     3061 tcctgggttt gtttgtctac gtttttaatc gcttgttcga tggcgtcaac gtcatcgccc
     3121 tgcgccactt cactcaggtg agcggcagca tcgtcaatga cctgacgttc tgcggcgctt
     3181 aacagcgcgg catcagcagc cagcgcgccg tgcagacttt ccagcacacg cgccgcttct
     3241 actttttgtt ctgccagcat tcgggctttt acgtcctgct cggcatagct cattgagtct
     3301 ttgatcatcg aagcgatttc gctatcggtc agaccgtaag acggtttgac ctgaatagac
     3361 gcctcaacgc cggtggattt ctccatcgcc gtcacgctca aaagaccgtc ggcatcgacc
     3421 tggaacgtca cgcgaatatg cgcaccacca gccggtagcg ccggaatacc acgcagcgca
     3481 aaacgcgcca gtgagcggca gtcctgcacc agttcgcgct caccctgcat tacatggata
     3541 gacatcgccg tctgaccatc tttaaaggtg gtgaaatcct gagcgcgggc caccggaatc
     3601 gtggtattac gcggaatcac tttctccacc aggccgccca tcgtttcgag gcccagcgac
     3661 agtgggatca catcaagcag cagcatttcg ctgtccggct tgttacccac cagaatatcc
     3721 gcctgaatcg ccgcgccaat agcgacgact ttatccgggt cgatggaagt cagcggtgga
     3781 cgaccgaaaa attcgcctac ccgttcacgc accagcggca cgcgagtaga accgcccacc
     3841 atcaccactt ccagcacttc atcagcttct acacccgcgt ctttcagcgc gcgacgacaa
     3901 gccagtaagg ttcgttttac cagtggcgcg atcagttcat tgaattgttc acggctgatt
     3961 tcgccctgcc agcccgcaac gttaacggtc acggagtccg catcgctcag cgcgattttg
     4021 gctgcaatgg cggcatccag cagttcacgc tgaacgcggt tatcgctacg atcaggaatg
     4081 cccgcctgct cgcgaatgta atccgccagc agatggtcga aatcatcgcc gccgagcgcg
     4141 gaatcaccgc cggttgccag cacttcaaac acgccgcgac ttaagcgcag aatggaaata
     4201 tcaaacgtcc cgccaccgag gtcataaaca gcgatcacgc cttcctgacc ggaatccagc
     4261 ccgtaggcga tagccgcagc ggtcggttcg ttaagtaagc gcagaacatg caaccccgcc
     4321 agacgcgccg cgtctttggt gccctgacgc tgggcatcgt caaagtacgc cggaacggtg
     4381 ataactacac catccagctc gcctgccagg gcttcagttg cccgcgccgc cagtgctttg
     4441 aggatgtccg cagaaacgcg caccgggttc agcagccccg ccgccgtttc aatcatcggc
     4501 aggccgtttt cgctggcctg gaattgataa ggcagatgcg gatagcgttg ctggatatca
     4561 gccagcgagc gccccatcag gcgtttaaca gaactaatgg tgttggcggt atcaagcgct
     4621 gcgttagtac gcgcgtcata acccaccgaa tgcccttgct gttgatagtg aacaacagat
     4681 ggcagcaggt gacggccttc atggtcggct aacgtttcgg cctgaccgct gcgcactgtc
     4741 gccaccagcg agttggttgt gcccaggtca ataccagccg ccagacgacg ctgatgcggc
     4801 gcagcactca aaccaggttc actaatttgt aataaggcca tgtttagctt ccagaaatta
     4861 aaaatcgagc agtttttctt cgagttgttc ggcactgctc cgcagtttat cgagaaaacg
     4921 cagcttacgc acggtatccg ccgccgcgtc ccacgcctcg ttgtctaact gttcaaccat
     4981 caactgatgg cgggtatcaa acatcttttt cacacgtttg ataaagcttt ccagccgcgc
     5041 ttcatctttc gcctgttcga tctcgtccag ctcttcgcgc agctccaact gttccatcag
     5101 gaacgcggtg tcgcgcacag tatgctgctc gctggcgaga tcaaaaccgt gtaaagaaag
     5161 caaatattcc gcgcgcatta acggatgacg cagcgtttgc caggcctggt taatggttgc
     5221 agattgctgt acggcggcga gttgttccgc ctggcttccg ctggcgaatt tatcaggatg
     5281 atactgacgt tgtagatcct gaaaacgcag gctcagcgcc tgggtatcga gttgataacg
     5341 ggcaggcaag ccaaagaggg tgaagtaatc cataacattc tcagggctgc gatccgccgc
     5401 gataacggcg aatcaggtaa aacaaacccc acgcgcaggc gaccacggtg gggttatcgg
     5461 tatgcgcatc aaacgtggaa gctttcgccg caaccacact catctttgac gttcgggttg
     5521 gtgaatttaa acccttcgtt caggccttct tttacgaagt ccagctgcgt accgtccaga
     5581 aattgcaggc ttttgccatc gaccacgact ttcacgcctt tgtcttcaaa cacgatgtct
     5641 tccggcgtcg gttcgtcaac aaattccagt acataagcca tacctgaaca tccggaggtt
     5701 ctcacgccca gacgcaggcc aaaccctttg ccgcggttag ccagaaaggt atttactcgc
     5761 gctgctgcac tgtcgctcag tgtaatcgac ataaccaaac ctcaactctt attttgcttc
     5821 acgtttgctt ttatagtccg caatggcggc tttgatcgcg tcttctgcca gaatagaaca
     5881 gtgaattttc accggcggca gttcaagttc ttcagcaata tcggtatttt tgatcgcctg
     5941 cgcttcgtcg agagacttcc ctttcaccca ttcggtgacc agggagctgg aagcgatagc
     6001 agaaccgcag ccgtaagttt taaaacgcgc gtcttcaatg ataccttcat cgttgacttt
     6061 aatctgcaac ttcatcacgt cgccacaggc cggtgccccc accatgccgc tgccgacgtt
     6121 ctcgtcgttg ttgtcaaagg aacccacgtt acgcggattc tcgtaatggt cgataacttt
     6181 ttcgctgtaa gccattataa attctcctga ttccgatacc gattaatgat gagcccattc
     6241 gatgctgttc agatccacgc cctgcttgta catttcccac agcggagaaa ggtcacgcag
     6301 acgaccgatg gatttacgaa ctaactcgat ggtgtagtcg atctcttctt cagtagtaaa
     6361 acgacctaaa gagaaacgga tagagctatg tgccagctcg tcgttcagcc ccagcgcgcg
     6421 cagcacgtag gacggttcga ggcttgctga cgtacaggcg gaacctgaag aaactgcgag
     6481 gtctttcagc gccataatca gcgactcacc ttcaacgtag ttgaagctga cgttgagaat
     6541 gttcggcgca ccgtgttcca ggtcaccgtt caggtaaact tcttcgatat ctttgatgcc
     6601 gttccacaga cggttacgca ggccgcgcag acgttccatc tcggtcgcca tctcttcttt
     6661 tgcgatgcga taggcctcgc ccattccgac gatctggtga acaggcagag tgccggaacg
     6721 cataccgcgc tcgtgaccgc cgccgtgcat ttgcgcttcg atgcgtacgc gcggtttacg
     6781 acgtacatac agcgcaccga tacctttcgg gccatagatt ttgtgaccgg agaaagacat
     6841 caggtcaact ttcaactggc tcaggtcgat aggcagtttg cccacgctct gggttgcatc
     6901 aacgtgatag ataatgccac gagcacggca catttcgccg atagccgcga tatcctgcac
     6961 cacgccgatt tcgttattta cgtgcatgat ggaaacaagg atggtgtcgt cacgcatcgc
     7021 tgcttcaagt tctttcaggt cgataatgcc gttacgctgc ggtgccaggt aggtgacttc
     7081 aaaaccttcg cgctccagct gacggcaggt atccagtacc gctttgtgtt cggttttgct
     7141 ggtgatgatg tgcttgcctt ttttctgata aaagttggct gcacctttga tcgccaggtt
     7201 gtcagattcg gttgcaccag aggtaaagac gatttcacgc ggatcagcgc cgaccagatc
     7261 ggcaatctga ttacgggcga tatctaccgc ttcttcagcc tgccagccga aacggtgaga
     7321 acgagaggcc gggttaccaa aggttccgtc catcgtcata aactgcatca ttttctcggc
     7381 aacacgcggg tccaccggcg tggttgcgga gtagtcgaga taaatcggta atttcattgc
     7441 tctataaact ccgtacatca ctcaatgcaa ggaaccaggc taccggctgg atgtacgacc
     7501 gtgtttacgg agcgcgatac gccccggcct gattctgaat tctttttatt aagcgcgtaa
     7561 cttaacgtcg atcgcgtctt gtatgcgggt gcgtggcgcg tcgtgagtat gctgacgacc
     7621 agacacatcc agcacttcct ggttattaac cagttcgcct aaagtaatgt tgttgagaaa
     7681 accggtgaga cggtcgctca aatcacgcca cagcgcgtgg gtcaggcatt tatcgccgcc
     7741 ctggcagccg cctttaccct gacaacgggt ggcatctaca gattcgtcaa cggcgctaat
     7801 cacttcgcca acggcgatgc tgctggcatc tttgcctaac agataaccac cgcctggtcc
     7861 acgtacgctg gaaaccagac catttttacg cagacgggaa aacagttgtt ccagataaga
     7921 aagggaaatt ccctgacgtt cggaaatatc agccaacggt accgggcccg cttcagagtt
     7981 gagcgcaacg tcaagcattg cggtcacggc atagcgccct ttagatgtca gtctcatgtc
     8041 ttacttcacc tcaaactcgc ccctgcccgg ggttttttat tgtaaaagtg ggggtattgc
     8101 atagcagggt caagtctgac attcccgagt aaattggtca actatttact tgactgattt
     8161 agtcgggtat ttaaccttca gtgccatttt tttatcgtgg cgtttgtacc tgttgtcctt
     8221 ccagaccatg ggcgggttga gggcaattat tcgcattagc gctggcctct tcccctcacc
     8281 ctaaccctct ccccgaaggg gcgaggggac cgtccgtgca tttatctggc ttagccctca
     8341 ggccttatta cctttattct gctgctcaat agaagccaga atcccgcgca ggatattcaa
     8401 ttcctggctt tccgggcgcg cacgggtaaa cagacggcgc aatttattca tcacctgccc
     8461 cggatggttt tcacggataa aaccggttgc cagcagcgtt tgttccagat gaccataaaa
     8521 acgctccaga tcatcgacca gcggatacgg cgtctcttca tgctcgacct gctcgccgtt
     8581 ttcctgagtt gccagccagg ccatgcgcac ttcataggca ataacctgaa ccgccatcgc
     8641 caggttcagc gagctgtatt ccgggttagc cgcaatcgcg acatgataat ggcatttctg
     8701 caactcttca ttggtcaagc cgacgcgctc acgaccaaac accagcgcca caggggtatt
     8761 tgccgcttca gcgacgcttt tcagaccgca ttcgcgcggg tcgagcatcg gccacggcag
     8821 cgtgcgggag cgtgcgctgg tgcccaccac caggctacaa ccggctaacg cttcgtcgag
     8881 cgtatcgacg atgtgagcat taccaatcac atcgctggcc cctgctgcca gggcaatcgc
     8941 ctgggagtcg ggtttcacca gtggattaac cagccacaga ttggttaatc ccattgtttt
     9001 catggcacgg gcaacagaac ccatattgcc ggtgtgtgac gtctccacca gcacaattcg
     9061 aatattttgc agcattgtct ttcttcatct aaagattatt cacgcatctt atcataaaac
     9121 gaagacagat gccgatctcg ctgctatact ctgcgccgtt ttcccgttct ttaacatcca
     9181 gtgagagaga ccgatgcatc cgatgctgaa catcgccgtg cgcgcagcgc gcaaggcggg
     9241 taatttaatt gccaaaaact atgaaacccc ggacgctgta gaagcgagcc agaaaggcag
     9301 taacgatttc gtgaccaacg tagataaagc tgccgaagcg gtgattatcg acacgattcg
     9361 taaatcttac ccacagcaca ccatcatcac cgaagaaagc ggtgaacttg aaggtactga
     9421 tcaggatgtt caatgggtta tcgatccact ggatggtact accaacttta tcaaacgtct
     9481 gccgcacttc gcggtatcta tcgccgttcg tatcaaaggc cgcaccgaag ttgctgtggt
     9541 atacgatcct atgcgtaacg aactgttcac cgccactcgc ggtcagggcg cacagctgaa
     9601 cggctaccgt ctgcgcggta gcaccgctcg cgatctcgac ggtaccattc tggcgaccgg
     9661 cttcccgttc aaagcaaaac agtacgccac tacctacatc aacatcgtcg gcaaactgtt
     9721 caacgaatgt gcagacttcc gtcgtaccgg ttctgcggcg ctggatctgg cttacgtcgc
     9781 tgcgggtcgt gttgacggtt tctttgaaat cggtctgcgt ccgtgggatt tcgcggcagg
     9841 cgagctgctg gttcgtgaag cgggcggcat cgtcagcgac ttcaccggtg gtcataacta
     9901 tatgctgacc ggtaacatcg ttgctggtaa cccacgtgtt gtgaaagcca tgctggcgaa
     9961 catgcgtgac gagttaagcg acgctctgaa gcgttaatga ctcaggcggg tgatgtcact
    10021 cacccgccct cgcctttcag gcgctattct gaaatacttc ctcaccgctt tactttcttt
    10081 cgtcactctc ccaccatctt tccccgatta atggatgata aagaagtcgc aaggaaaggt
    10141 tatggcactg ccagtgaaca aacgcgttcc caaaattctg tttattctct ttgttgttgc
    10201 cttctgcgtt tatttagtgc cgcgcgttgc catcaacttc ttctattatc ctgacgataa
    10261 aatttacggt cccgacccct ggtcggcgga atccgtcgaa tttacggcta aggacggtac
    10321 tcgtctgcaa ggctggttta ttccttcttc gacgggccct gctgacaacg ccatcgcaac
    10381 catcattcat gctcacggca atgccggaaa tatgtccgcc cactggccgc tggtcagttg
    10441 gttacctgag cgtaatttca acgtttttat gtttgattat cgcgggtttg gtaaatcaaa
    10501 aggcacgccg tcccaagccg gattgctgga cgatacgcaa agtgccatca atgtggtgcg
    10561 ccatcgcagt gatgtaaacc cacaacgtct ggtgctgttc gggcagagta ttggcggggc
    10621 gaatattctg gatgttattg gtcggggtga tcgtgaaggc atacgtgcgg tgatcctcga
    10681 ctccacattt gcctcttatg caaccatcgc caaccagatg atccccggca gtggctactt
    10741 acttgatgag agttacagcg gcgaaaatta tatcgccagc gtcagcccga tcccgctttt
    10801 actcattcac ggtaaagctg atcacgttat cccatggcag cacagcgaaa agttgtatag
    10861 cctggcaaaa gagccaaaac ggttgatcct aatcccggat ggcgaacaca ttgatgcttt
    10921 ttccgatcgt cacggcgatg tttatcgcga acagatggtg gactttatcc ttagcgcgtt
    10981 gaatccacag aactaaccca tgatcgctag cacgataatc attcacaaaa ccaccttaag
    11041 acatgctaat ccactggtca gaacagttta agatgagaaa aattctgtga cgcttgccaa
    11101 catttctgat gattagcatt cccttcgcca tttccctgag caaactttag ctattcttat
    11161 caattatgct tatgggagat ctacagatga tgcctacgct tgctccacca tctgtccttt
    11221 cggctcccca gcgccgctgc cagatcttgc tgacgctctt tcagccgggg ttgaccgcca
    11281 ccacggcaac tttcagcgag cttaatggtg tggatgatga tattgccagt cttgatatca
    11341 gcgaaacagg acaggagatc ctgcgctatc atcaactcac actgacggct ggttatgacg
    11401 gtagctaccg ggttgaaggt acagtgctta accaacgttt gtgtttattt cactggctac
    11461 gacgtggttt ccgtctgtgt ccatcattta ttaccagcca tttcaccccc gccctgaaga
    11521 gtgaactgaa gcggcgcgga attgcgcgta acttttacga cgataccaat ctacaagcgt
    11581 tagtgaatct ctgctcccga cgactgcaaa aacgctttga aacgcgcgat attcatttcc
    11641 tgtgtctgta tctgcaatat tgtttgctgc aacaccacgc tggaattacg cctcagttta
    11701 atccgctcca acgtcgctgg gcggaatcct gccttgaatt tcaggtagcg caggaaattg
    11761 gacgccactg gcagcgtcgg gcgctccagc ctgtaccgcc tgatgagcca ctgtttatgg
    11821 cactactttt ttccatgttg cgggttcccg atccactgcg ggatgcgcat cagcgagaca
    11881 gacaattgcg tcagtctatc aaacgtctgg taaaccattt tcgtgagctg ggaaatgttc
    11941 gtttttatga tgaacagggg ttatgtgatc agctttatac ccacctcgcc caggcgttaa
    12001 atcgcagttt gtttgccatc ggtattgata ataccctgcc ggaagagttc gccagactgt
    12061 acccacgcct ggtgcgcacc acccgcgcgg cgctggccgg atttgaaagt gaatacggcg
    12121 tccatctttc tgatgaggaa agtggtctgg tcgcggtgat tttcggtgcc tggctaatgc
    12181 aggaaaacga tctgcatgaa aaacagatta ttctactgac cgggaatgat agcgagcgag
    12241 aagcgcagat tgagcagcag ttacgcgaac taacgttact gccgctcaac attaagcata
    12301 tgtcggtaaa ggcatttttg cagacaggcg ctccgcgtgg cgcggcactg attattgcgc
    12361 cttataccat gccattaccg ctcttttcac caccgctgat ctatacggac ctgacgctga
    12421 caacacatca acaggagcag atccgcaaaa tgctcgaatc agcatgaggg aacaactttc
    12481 gggcgcaaaa acattgccgg cagcgccacc agcgccatta cccagaacac gccgtggccc
    12541 agatattgat acaggaaacc ggcgaaaacg gtcatgatag cgatactgcc gcccatcgcg
    12601 acggcagagt aaaccgcctg taaacggatg acttcgctac cctggcgagc agcaatataa
    12661 cgcatggcgg ccaggtggca gaccgtgaag gtgccgcaat gcagaatttg caccactatc
    12721 aaccacggca acgccgtagt tgctcccata atgccccagc gcactacgcc gcaaatcgcc
    12781 gagatcaaca gcatatcgcg tgcactacaa cggcggaaaa gtttattact cagcgcaaag
    12841 ataatgactt ccgccaccac gcccagcgac cacaaatacc ccaccgccga ggccgagtag
    12901 ccagcagcct gccagtaaat ggcgctaaaa ccgtaatagg ccgcatgtgc cccctgcaat
    12961 aaacaaacgc aggccagaaa gcgccagttc tggcgaacca gcgccaacca cgcagaccaa
    13021 ccggtgctct cctgctggcg acttgcccct tgtggctgaa tcgtcggacg gatgagaaag
    13081 ccgagcagca tggatgccac gcccaacgtc aacagcgcga ggatcacccg ataatcaaac
    13141 atagtgacca gtttgcccgt cagcgccgag ccaatgacaa acgccaccga gccccacagt
    13201 cgcactttgc cgtaatcaag cgggaactgc ttttgccacg tattcgccag tgcatcggtc
    13261 aacggtacca gtggtgagaa aaagaggtta aagccaatca tcaccagcat cagccacgct
    13321 acgtgcgccc ccgcccagaa ggcgacagca aagagaagtg tcagcagtgc cagcacgcgc
    13381 aaggcggaaa tcaggcggga aggatcgctg acgcggggcg cgatgagcaa actcccgagg
    13441 aaacgggcaa ccagacctgc ccccaataac aggccgatgg tttctggcgt taaaccaatc
    13501 cctttaagcc agacgctcca gaaaggtaga aaaatgccgt aactaaaaaa gtatgtgaaa
    13561 tagccgagcg ccaaccagcg cgtggattgc aaaaccatga gtccctcccg tcaggaggcg
    13621 ttagtctggg gttaatcgaa tgttggtgca agttgaaaaa taagaatatt cgagcctgct
    13681 tcccgatgtc tgtaacgcca gttcacaagc actaaaaaac tgcgatccgc gacgcatgtt
    13741 gcaaaaataa tgcgtaatta tgccgttacg cttgccaaac gttcctgcac aatggcgatg
    13801 aaatcgcgta atgccggctt catctcccct ttcttccacg ccatcagtaa agcaatggaa
    13861 ggcacatttc cggcaatggg acgaaaaaca acctgcccgg tattaaaatt attcatataa
    13921 ccgggtatca aagtgacgcc cagccccatg cccaccagat tcatggtcac cagaatattc
    13981 gttgccacct ggacgatatt tggctggctg ttttcttgcg caaaccacgc tttaacgatc
    14041 ggcgcaagcg aaccggaata cgccggatcg gtactgacga aattcacgcc atccagttgc
    14101 gcggcggtga tctctttttc atgtgctaaa gggtgatcaa ccggtaacac aaccactaat
    14161 ggttcgtcaa aaagctccag ataatcaatc tccgggctat aaacaggatg gcgcattaag
    14221 ccgacgtcga gttcaccacg gcgaattttt tcctcctgtt gcgtggtgat taaactcacc
    14281 agctcaatca aggtgtctgg ctgtctgaga cgaaacatcg gtaatacttt tggcagtaaa
    14341 ttcacttccg ccgatggcac aaagccaatg gttaattgtc tgtcttcctg aacaattttc
    14401 cgcgcccgta atttggcatt ttccgcttgt tcgaggattg ccagcgcgtc ctggagaaaa
    14461 cattctcccg ccgccgtcaa cgcgactttg cgcttatccc tcaccagtaa cggaacaccg
    14521 acacagtttt caagatcgcg gatctggctg cttaacgaag gctgtgaggt atgcagtttt
    14581 tccgccgcac gggtaaagtt cagtgcctgc gccactgcga cgaaatagcg taaatgccgt
    14641 agttccatca ccttcccctt gttatcgaaa aaacgtctca gctggtagaa attaactatt
    14701 tcacatatta gcaaccaacc agcaacatcc ttatggcaca aaaatagaag gtcaatacat
    14761 cttatctttc aggattaaaa aatatgacca caccctcaga tttgaacatt taccaactga
    14821 ttgacaccca aaatggtcgg gtcactccgc gtatttatac cgacccagat atttaccaac
    14881 tggagcttga gcgtattttc ggtcgttgct ggctatttct cgcccacgaa agccagatcc
    14941 caaaacccgg tgatttcttt aacacctaca tgggagaaga tgcggttgtc gtagtgcgtc
    15001 agaaagacgg cagcatcaag gcgtttctca accaatgccg ccaccgggcc atgcgtgtga
    15061 gttatgcaga ttgcggcaac actcgcgcct ttacctgccc gtatcacggc tggtcttatg
    15121 gcattaacgg cgagttgatc gatgtaccgc tggaacctcg cgcctaccca caagggttgt
    15181 gtaaatccca ctggggacta aacgaagttc cttgtgtgga gagttataaa gggctgattt
    15241 ttggcaactg ggataccagc gcaccgggcc tgcgtgatta cctgggtgac attgcctggt
    15301 atctggatgg catgctggat cgtcgcgaag gcggcaccga aattgtcggc ggcgtacaaa
    15361 agtgggtgat caactgtaac tggaaattcc cggcagagca gttcgccagt gaccagtatc
    15421 atgctctgtt cagccatgct tctgccgttc aggtattagg ggcaaaagat gatggcagcg
    15481 ataagcgcct cggtgatgga caaaccgccc gcccggtgtg ggaaaccgcc aaagatgcgc
    15541 tgcaatttgg tcaggacggt cacggtagcg gtttcttctt tactgaaaaa ccggatgcta
    15601 atgtctgggt cgatggcgca gtttcaagct attaccgcga aacctatgcc gaagcagaac
    15661 aacgtttagg tgaagttcgc gccctgcgcc tggcgggtca taacaatata ttccccacgc
    15721 tttcatggct aaacggcact gccacactcc gcgtctggca tccgcgcggc cctgatcaag
    15781 ttgaagtgtg ggcgttctgt attactgaca aagccgcctc cgatgaagtt aaagccgctt
    15841 ttgaaaacag cgccactcgt gcttttggtc ctgctggttt tctcgagcag gatgactcgg
    15901 agaactggtg tgaaatccag aaattgctta aaggccaccg cgcccgcaac agcaaactgt
    15961 gtctggaaat ggggcttggt caggaaaagc gccgcgacga cggcattcct ggcattacta
    16021 actatatctt ttcagaaact gccgctcgtg gaatgtacca acgctgggcc gatcttctga
    16081 gtagcgaaag ctggcaggaa gtgctcgata aaaccgccgc ttaccagcag gaggtgatga
    16141 aatgagtgcg caagtttcac tagagttaca tcaccgcatt agccagtttc tctttcacga
    16201 agccagctta ctggacgact ggaaatttcg tgactggctg gcgcagctcg acgaagagat
    16261 tcgttacacc atgcgcacca cagttaacgc gcaaacacgc gaccgccgca aaggcgtcca
    16321 gccaccgaca acctggattt ttaatgacac caaagaccag ctggagcggc gaatcgcccg
    16381 tctggaaacg ggcatggcat gggcagaaga gccgccgtca cgcacccgcc acttaatcag
    16441 caactgccag ataagcgaaa ccgacatccc aaacgtattt gctgttcggg tgaattatct
    16501 gctttatcgg gcacaaaaag agcgcgatga aacgttctat gtcgggactc gttttgacaa
    16561 agttcgccgt ctggaagatg acaactggcg cttgctggaa cgggatatcg tcctggatca
    16621 agcggtaatc acttcccata acctgagtgt actgttctga tgaatcgaat ttatgcgtgt
    16681 cccgttgcgg atgtgccgga gggtgaggct ctccggatcg atacctcgcc cgtcatcgcc
    16741 ctgttcaacg ttggcggcga gttttatgcc attaacgatc gttgcagcca tggtaatgcg
    16801 tcaatgtcag aagggtatct ggaagatgac gccacggtgg agtgcccgct acacgccgcc
    16861 agtttttgcc tgaaaacggg gaaagcgtta tgcctgcccg ccaccgatcc gctcaccact
    16921 tatccagtac acgttgaagg tggtgacatt ttcatcgact taccggaggc gcagccatga
    16981 gcgatctgca taacgagtcc atttttatta ccggcggcgg atcgggatta gggctggcgc
    17041 tggtcgagcg atttatcgaa gaaggcgcgc aggttgccac gctggaactg tcggcggcaa
    17101 aagtcgccag tctgcgtcag cgatttggcg aacatattct ggcggtggaa ggtaacgtaa
    17161 cctgttatgc cgattatcaa cgcgcggtcg atcagatcct gactcgttcc ggcaagctgg
    17221 attgttttat cggcaatgcg ggcatctggg atcacaatgc ctcactggtt aatactcccg
    17281 cagagacgct cgaaaccggc ttccacgagc tatttaacgt caatgttctc ggttacctgc
    17341 tgggcgcaaa agcctgcgct ccggcgttaa tcgccagtga aggcagcatg attttcacac
    17401 tgtcaaatgc cgcctggtat cctggcggcg gtggcccgct gtacaccgcc agtaaacatg
    17461 ccgcaaccgg acttattcgc caactggctt atgaactggc accgaaagtg cgggtgaatg
    17521 gcgtcggccc gtgtggtatg gccagcgacc tgcgcggccc acaggcgctc gggcaaagtg
    17581 aaacctcgat aatgcagtct ctgacgccgg agaaaattgc cgccatttta ccgctgcaat
    17641 ttttcccgca accggcggat tttacggggc cgtatgtgat gttggcatcg cggcgcaata
    17701 atcgcgcatt aagcggtgtg atgatcaacg ctgatgcggg tttagcgatt cgcggcattc
    17761 gccacgtagc ggctgggctg gatctttaag gaagcacgat gaaagaaaaa acgatcatta
    17821 ttgtcggtgg cgggcaagcg gcggcaatgg ctgcggcctc gctacgccag caagggttca
    17881 ccggtgagct gcatctgttt tccgatgagc gacatcttcc ttatgaacga cctccactct
    17941 cgaaatccat gttgctggaa gattccccgc agttacagca ggtgttaccc gctaactggt
    18001 ggcaggaaaa caatgttcat ctgcattccg gtgtaaccat caaaacgctg ggtcgcgaca
    18061 cacgagagtt agtgttaacc aacggcgaaa gctggcactg ggatcagctt tttatagcaa
    18121 ccggcgcggc agctcgaccg ctgccgttgc ttgatgcact gggagaacgc tgctttaccc
    18181 tacgccatgc cggtgatgcc gccagactgc gagaagttct gcagcccgaa cggtcagtcg
    18241 tgattgtcgg tgccggaact attggtctgg aactggctgc cagcgccacg cagcgcagat
    18301 gtaaggtgac agtgattgaa ctggcggcaa ccgtcatggg ccgtaatgca ccaccgcccg
    18361 tgcaacgcta tcttttacag cgccaccagc aggctggtgt gcgcattctg ctcaataatg
    18421 ccattgaaca tgtggtcgat ggtgaaaaag tagaactgac gctgcaaagt ggggaaacgc
    18481 ttcaggctga tgtggtgatt tacggtattg gtatcagcgc caacgagcaa ctggctcgcg
    18541 aggccaacct tgatactgcc aatggcattg tcattgatga ggcttgccgc acctgcgatc
    18601 ccgcgatctt tgccggtggc gatgtggcaa tcactcgtct tgataatggt gcactacacc
    18661 gctgcgaaag ctgggaaaac gccaataacc aggcgcaaat tgccgctgcc gcaatgttag
    18721 ggctaccgct accgctactg ccgccgccgt ggttctggag cgatcagtac agtgataact
    18781 tacagtttat tggcgatatg cgtggcgatg actggctttg tcgtggcaac ccggaaactc
    18841 agaaggcgat ttggtttaat ctgcaaaacg gcgtgcttat cggtgcggtc acgctgaatc
    18901 aggggcgtga gattcgccca attcgcaaat ggatccagag cggcaaaacg tttgatgcga
    18961 aactgctgat agatgagaac atcgcgctta aatcactgta accaggataa ttagcgaata
    19021 tctcaatgcc tggggcgtgg cgaggtgcaa gagtgtgtat tacgcttaaa tcacattatc
    19081 ttgcaaaggg attggttatg aacacactac gttattttga ttttggagct gcccgccccg
    19141 ttttgttatt aattgcccgt atcgccgtgg tcttaatttt cattattttt ggttttccca
    19201 aaatgatggg ctttgacggt acggtccaat atatggcctc gttgggcgcg ccaatgccga
    19261 tgctggcagc gattattgcg gtagttatgg aagtgcccgc cgcgatatta atcgtgcttg
    19321 gctttttcac ccgtccgctg gcggtgctgt ttattttcta cacgctgggt acggcggtga
    19381 ttggtcacca ttactgggat atgaccggcg atgcggttgg gccaaatatg attaatttct
    19441 ggaagaatgt cagtatcgct ggcgcattcc tgctgctggc aattaccggg ccgggggcaa
    19501 tttctctcga tcggcgttag gaaagatgcc ggatgcggcg tgaacgcctt atccggcatt
    19561 taataaatta cagccactct acccgcaacg acatctctga agttgttgat tcccctggtg
    19621 ctagcgcaat gagatcaccc ccttccgggc gatgatgatc atccggcgca tggctcatcg
    19681 gttccagaca gaagaaatca aacgcatatc ctttatcaaa cgcagggtcg gaaacaaaga
    19741 tgaaataaca cggtgcaggt ggcgtcgttt ccatgatgat ggcataacct tcctgcggct
    19801 gctcgatgcg ggcctgacca ttccatccgg caaaaccatt gttcacccac tggcgcggca
    19861 acggcgcggg ctggttaaaa tccagttcct gcggtagctg ctcgcaaaac tcacccgcca
    19921 gccactgctc ccgctccagc cagtaaccgc tcgcctgtgc ctgaatccgc gtttgcggcg
    19981 acaacggaaa ataaggatgc cagccggtac caaatggcag cgtctctgcc ccttgattgg
    20041 tgacagagag cgtcaccgtc agcgtatccg ccgttaaatg aaacgcctga cttacccgat
    20101 agtgatagac accgctgcga tgttcataca ccagacacaa actatcatcg ctgtgcgaga
    20161 cacattgcca ctcgcccagc cagccatcgc cgtgtagata gtgcgcatcc cactcaacat
    20221 tcggttgcag ttgatactca cgcccctgcc agacaaaacg attgccgctc acccggtttg
    20281 caaatggcac cagcggaaaa catgaggcat cggttgccac accgcttttt ttaccaggac
    20341 gtaagagcgg cgtcgtatcg cgccagaagc cttcgattac gccgccctgg tcagaaacgt
    20401 ccagctttag cgacccgtgg gataaggtat agatggtcat ccgataactc cttaatccgg
    20461 gaagttaatc acaactttcc cgcatttgcc gctcgccatc agcgcatagg catcacctgc
    20521 ctgttccagc gagaagcgat gggtaatggc gttacgcggc cacagtttcc agtccgtcag
    20581 atcatgggcg catttttcca tatggaacag actggtcacc caggagccga taatccgccg
    20641 ttgatggtgc atcagatcgg cgctgacctc gaactccact tttccggttt ctccgatgta
    20701 aaccacccgt ccccagtcag cggtggatcg cagtgccagc aagcgacctg cggcattacc
    20761 agaacaatcg agcgcaacat ccgcgccacc gtgggtgagt tcggcgataa tctgcggcag
    20821 accttcggtg gttgctaaat agccgtgatc catcacccct aactgttttg ccatcgccag
    20881 acgttccggc agcatatcaa cgccgatgat ccgttttgca ccgcgacctt tcgccagcat
    20941 catcgccatc atgccgactg gccccagacc gaccaccagc acgttatcac tgccggaaac
    21001 ttcgccgcgc aaaatccctt cataggccgt gccaacgccg caactgataa acgcaccatc
    21061 ttcgtagctc agcgcatccg gcaggaggat cagatctttt tcttccgcca gcagatattc
    21121 ggcatgaccg ccgtcacgct gccagccgta agccgctttt cctttgccag tacaagagat
    21181 aggaaagccg cggcggcagt tcgggcaaaa accacagcca gaaatgtgat acaccagcac
    21241 gcgatcaccc tctttaaaat ggcgacagcc ttgccccatc gccacaatct gcccgcacgg
    21301 ttcatgaccg ttgataaatc cctggtataa cggtttatcg ggtgccgccg ccgtggcacg
    21361 gtgttgatga tagatatagt ggacatcgct tccgcaactt cccgcagatc gacggttgaa
    21421 tttcctggta aataagctgc cagcatcgtt ttcataaatc cctcattaac aatacgatta
    21481 attttcatcc ctgccgcacc cgcgccaggg gcgttaaggt ttagcgtttc gctttactgc
    21541 ttcgctgggt cagcaagata ttcgccagca ccgccaccac gatgatgacg ccgcgtacca
    21601 cctgctggaa aaaggagtta ataccgagca gcaccagacc gttaccgatt agcgtaatca
    21661 ccagcacacc aagcaatgta ccgaacaggg aaccgcgacc gccggaaagt gccgtaccgc
    21721 cgaccacgac cgcggcgatg acgtcaaact ccagaccgtt tgcggcacct gcgttaccag
    21781 aaccgaggcg cgccgccaac aaaatgccgg tcaccgccgc taataatccc gaaagggtaa
    21841 agataagaat gcgcacccga cgaacgttga tgccgcacaa ctgcgccgcc gtagcattac
    21901 cgccaacggc aaaaaccgag cgcccgaagg cggttttgcg gctgatgaac acaaacagcg
    21961 caaacaacac aatcatgatc agcgcggata ccggcacacc gagaaattgt ccgcccagcc
    22021 agtccagcac ctcgttttcg tcaatcggca ctggcagtgc gttcgtcata aacagcccca
    22081 ttccgcgcag ggcgctccac agccccagtg tggcaacgaa acttggtacg ttaaacacgc
    22141 cgcgcagcac cccggccagc gttcccatca gcgcgcctaa cagcaacacc agcagacacg
    22201 ccaccgccag cggaacttca aattgcagca aaaatgccag gcacaccgag acaaaagcca
    22261 ccatcggccc aacgctgaca tcaatttcac cggagataat aatcagcgtc atcgcccagg
    22321 cggcaatccc aatggtggcg gcatcgcgca gcacgttcat ctggttattc aatgagataa
    22381 agccaggcgc gttcagggag aagaccagat aaagaatggc gattaccacc agcaaaccga
    22441 tctcattaat atggcgactg acaaattgtt tgagcgagac gctcttgccc tgcggcaatg
    22501 ataatgacga agcagacatc ttcaggttcc tcggtaaaat tcatcagtgc acagacagaa
    22561 tggcggacat cagctcatcc acattgaccg gcgagtgaaa ctcctgcgag aacgtaccgt
    22621 gctgtaacaa caggatgcgg tcgcacacca gcggcaactc ctccacttca ctggagataa
    22681 acaccacgct ttttccttcg gcagccagct cacggacaat acggtaaatc tgctgtttgg
    22741 cttcgatatc gacgccgcgc gttggctcgt cgagcaacaa aatctggctg gcggcataga
    22801 cccaacgacc gatcaccact ttttgctgat tgccgccaga aagcgtgccg atgggcgttt
    22861 cgctactggc ggccttgacc gtcatccgct gcatcacctc ttcggtcagg cggcggatgg
    22921 tggaccattg cagtacgccg ttggcgctga ttttttgccg attggtcagc actgtatttt
    22981 cgtcaacgcc caaccaggga atgatccccg cttctttgcg gttttctggc gtatagccaa
    23041 tgccgcgttt cagcatgtcg ccgtaatcgg ggcgcgtgat tttctcgccg ttgataacaa
    23101 tttcgccctg ttcatactcc tccagcccaa caatcgcctt cagcaattca ctgcgccctg
    23161 cccccagcag gccagcaatg ccaagcactt cgccgcgacg taacgtaaag ctgatatcct
    23221 ccagcttggg cttatggcgt aatgcacgga cttccagcac ggcctgatcc acaatttcct
    23281 gaggggctac cggcgcaata tcaacgtgat cacgccctag cattagcgac acaatatgat
    23341 gcgtggaggt gttttcgagc atcacatcgc ccgccacctg accatcgcgc ataacggtgg
    23401 cacaggaggc aatgcggcgg atttcttcca tccggtggct gacataaatc accgccacgc
    23461 ccagtgccga catctttttc accgcactga tcaccagttc aacttctgca ctggcaagcg
    23521 agctggtggg ttcatcaaga atgaccacgc gcggctcgcc cttcatcacc cgtgcaattt
    23581 ccaccagttg cttttgcgcc gggcttagcg ttgaaacaag ttgttcagga ctaacgtcaa
    23641 cgcccagcgt ctgtaagcaa cgttgggcat cctgcgccat ttgcaggtaa tcaatcatgc
    23701 cgttgcggcg aggccattga ccaaggcaga ggttttctgc caccgtcagc ccttccacca
    23761 gacttaattc ctgataaacc gcacgaaccc ccagttcagc ggcacggcga gtcaacgtag
    23821 cttcgtcacc ttccagtcgc gtctcgccaa tccagatatc accgctatcc gggcgttcgc
    23881 tgccggtaag cattcgaatg agagtcgatt tgcccgcgcc gtttttacct aacagcgcac
    23941 gaacttcgcc tttattgagc gtgaagttaa cgttatccag cgcaacgacg ccggggtaac
    24001 gcttatttcc tgccaccact tttgctaccg ggactgcctc tgttgccgtg aacataggga
    24061 cctctgcgaa tcagcgatta gggcagacca tcaacgtgcg ttgccagcca ctgtttacca
    24121 tcttccgttt tggtatagag atcgattggc acctgaatca ctttttcacc gtcggcttgt
    24181 ttattgataa ccttcaatgt ttgcgcgaaa acagcattgc ccattttctt accggaaata
    24241 tccactaccg ctttcagcac ctgattgttt tccagctcct gagcaatttc ggttgtcata
    24301 tccgaaccga aaacagcaat ttttccggcc tgattttgat tacgtaccgc ttttaccgcg
    24361 tcgagtgtcg caccgcccga ctcccccata atggcgttga gatccggcgt ggagataatc
    24421 agtttttcac caacggaaat cgctttatct aaaacagtcc cttcctgatt agcgacaatt
    24481 tgcgcgccgg gaacgcggga ttttaatact tcttcaaatc ctttacgtcg ctgcacacaa
    24541 acttcaaagg cttcgcaatt gatgacggca attttcggct ggtcaatttt attggcaata
    24601 aaataatcgg cggcagcgtt acccagtttt ttaccaaatt ccagtggatc gccgaccaga
    24661 tacgccgaga catatttatc gacacccttt tgattaatac aggtgttgta gcaaatcacc
    24721 ggaatacccg cttcactggc gcgacgaacg gtacggctac tgccattttc agacactgcc
    24781 gataaaataa tggcatcgac attacgcgcc acgagggtat caacaaaggt actttctttc
    24841 gaaatatcac cctgggcgtt agtttcaatt aactgtacct gtactgaaga atcttttgcc
    24901 gcatcctgaa cgccctgacg cactccagcg taatatccct gggtatcaag gtatattgca
    24961 ccaatggtca tttctttatc cgcagccctg gcaaatagtg cgctacctaa tagcgtaccc
    25021 attaataata gattacgggt tgttctcatt tttttaggca tagtgcctcc tgtagggttt
    25081 ttattaacaa cggcttattc taattatttt gtgatgagcg gcagcgcgtg ccgctcaaat
    25141 attacagtta atagggttta gtgaacgtag ttaaaaataa aaggcatcac ggtggtgaat
    25201 aatgccgctt tcggccaggc cggattaatc gccgtcaatt cagcgcgagc agattcatat
    25261 tgcgcaacct ccccgagtcc cgcggacgcc agcatcgcca ccatcaggca tttttcttta
    25321 tgctgctgtt gtaaatcgcc atacagcgac aacaggtcag gttgtgaaac agcaaagaaa
    25381 tccgcctcaa tactggtttt cgccatctct tgcgcccact gtttcatttc actaaacagt
    25441 tgctgtgcgg tttgctgttc acccagcagt cgcagcgcca ttccttgcca gaagagataa
    25501 tcaaccggct gatcgttgta ataactgtgg atgttaatgg tgcgatcgcc ggtcgccgcc
    25561 agacgtaaac aacgcatcgc ttcagtttca tcgccctgcg cgttggcgca tatcgcctgc
    25621 cagaaccaga tgtcgttatc agtttgcccc ggtaaacggc cttcgcttaa attctccgga
    25681 taatgcagcg cggcatgaag cagttcgctg gcctgctgcg gctctctggc atcaagatgc
    25741 tgccaggcgc gtaataactg gttgaggata aactgactgg tgaccttccc ttccccgcct
    25801 tcccacgggt ggaatttgcg cgtggcgaga atgtccgccg ctttgtctgc ctgacccgtg
    25861 agatgccaca aattgagcaa ctctgctgtc atgtcgtcgc gtttcagcgc aatttccaga
    25921 ttattttcca gacgcgccag tcgtttctcc ggtgtggctc cgcttaactt atcaagcaaa
    25981 tcccgttcga aaagcagacg tgcatcctgc ggcgcaagct gataagcatt atcaagataa
    26041 cgcgcggcca gctcataatc gtgttgctta ttccacgcat ggatcgctaa cccgcgccag
    26101 ccgtcggcaa actccggcga catctctacg caacgttgcc ataaggtaat ggctttgttg
    26161 tagctacgct tgttgtagta gaagcaggcc agtaaatgac gggcaaacca gcactcttca
    26221 atactctcca gcgccgccac ttcttccagc gtattcggga aacggacaaa ctgcgggaag
    26281 acatcaatgg cttttgcgac cagttcgccg cgttcggctt tcggcaataa actcgcctgc
    26341 aggtaaagcg gcagcgtgcg ctggcagtcc agtgcgttca gcatctctgc cgccagggtg
    26401 ggcattcccc agttaatcag ttgcccggcg gtcatcagag cgttaacgtc gcgtccctga
    26461 cacagaccgc gccactgcgc gagagctgat tcgctacgac catcgaacca gttcagccac
    26521 cacagagtgg cgttcagcgg ataatcgcgc agcagtttct cgcgctgcac acgcgcgttg
    26581 tcctgacgac cacttaacac cagcagcaga ttatgcaggc aaagcacttc ctgattggtt
    26641 gggcaggcgc gaagactttg ttggcaaaaa tccagaccag cgtcgaagtt accattacgc
    26701 gccgccagtc gtgccaggcc ataatagcca ccggctttgc tgttgccgct ccagaccgca
    26761 cgccagaaat cctcttcggc ttgttgatat tgtccctgac gttcgtaagc actggcgcga
    26821 atcaaactgg cctgtccgca ctgcggattt ttgttcagcg catgtgcgcg tttcagcgcc
    26881 tgagtggcat acgccaccgc ttgcgggaaa tcggcgcggt tatattccag catcgccagc
    26941 gccaggttac agcggtaatc cagcggatcc agcaccacgc cgcgcaggta gtaatcgaac
    27001 ggtgaacggc tggcgtgatg atattgctcc agatgctgac cgataaacca ggcttcatct
    27061 gtactggtaa tgtcttgtgc tgccagtggc gctttggcga cgtccggcaa cggcaactct
    27121 tgcggctgat gttcctgata actcagtaca atattgccgt cggcatcgga gagctcaatg
    27181 gtcagccttt caggattgat accgtgcaac acgccctgga tggcggtcgc aggcatcagt
    27241 gccacggcat catcaagtaa cgcgttgcat ttgccgattt cgcggatcgc caggcggtat
    27301 ccgttcaacg gagagatggc atacagcccc cactcaatcc cccgcttact acgctggagt
    27361 tttatcaccg catcgcggga ggcattttgc accatgccca aagaatgata aggcaggaaa
    27421 tactgctcaa aacgcttctc ttcgtaagca tcaagccagg taaaatcggg ctggttatcg
    27481 gcaaaaatac cggtcatcag ttcgatatac gggccgttat tgtcggtcag actcttatcc
    27541 cacgcctggc caaattcact gtgtccccaa ctccactgtt ttttacctgg cgcgatatgg
    27601 tggttggcaa cgtgcagcaa accgccatct tcatcgtgac accacgcgcc gacaaaatcg
    27661 tactgcgatt tttcggccat atatgaggtt ggaacaggca catttttata gcgagaaatg
    27721 tccactccgg cggagtagtc cactttgtag taagtgccgg tggcgatggg gaaagcggag
    27781 acggcccgtt tgccgtgatc aaacaccgca gtcacatccg gtgggaaaac gctctgatgc
    27841 ccttcccccc ctttcactgc cgggttgacc caccacaaga aatgacgcgg cgtggcgtta
    27901 ccgttataga cgcggctggc gatttccagc gccgcccggt cagggcgcag ggtgaaacct
    27961 gtcatcacct gtaaaccatg catcggctcc gtttcgccta cccacaccgt ctgtgcaccg
    28021 tcttcatggg cttcgagggt gaaatcaacg ggcataaagg tggtcgggcg atgatgttgc
    28081 ggccagttaa actcaatccc accggaaatc cacggcccca gcagccccac cagcgcaggt
    28141 ttaatgactt cattgtgata aacaaaatcg cgctgtttca ctttatccca cgcgcgatgc
    28201 acccgaccgc ccagttccgg caggatcatc actttgatgt agtcgttttc cagccacacc
    28261 gcctgccagg atttcagggt tttctgctcg ctcagcgtat cggtcacgcc gtagggataa
    28321 accgcgccgg acgatccctg ataaacgcga ttttccagga acatgggatg tatatcctgc
    28381 ggcccggttt cataggtcgg gatctcaacg cgctcttgcc acacttttac tggagtcatg
    28441 gtgccctcaa tattaacaag acatactgaa ttaaaagatt tgtggcagtg tattgaacaa
    28501 tctggcaatg ttttcgcgga ataatcacgc aattaactaa acaaggttta gtgaagatga
    28561 gagcctgcat taacaatcaa cagattcgcc accataacaa atgcgtgatt ctggaactgc
    28621 tgtaccggca aaagcgcgcc aataaatcaa cgctggcccg gctggcacaa atttcgattc
    28681 cggcagtcag taatattttg caggaactgg aaagcgaaaa acgggtggtg aatattgacg
    28741 atgaaagcca gacgcgcggg catagtagcg gtacatggct gattgcgccg aaaggtgact
    28801 ggacgctgtg cctgaacgtg acgcccacca gtattgagtg tcaggttgct aatgcttgtt
    28861 taagtccgaa aggtgaattt gagtatttac agattgatgc accgacgccg caggcgctgc
    28921 tgtccgaaat cgaaaaatgc tggcatcgcc accgtaaatt gtggccggac cgcaccatca
    28981 atctggcgct ggcaatccac ggtcaggttg atccggtgac cggcgtgtcg caaaccatgc
    29041 cgcaagcgcc gtggacaaca ccggttgagg tgaagtatct gctggaagag aagctgggca
    29101 ttcgggtgat ggtcgataat gactgcgtga tgctggcgct ggcggagaaa tggcaaaata
    29161 attcgcagga acgggatttc tgcgtgatca acgttgatta cggcattggc tcgtcgttcg
    29221 tgattaacga gcaaatttat cgcggcagtt tgtatggtag cggacagatt ggtcacacca
    29281 tcgttaatcc ggatggcgtc gtctgcgact gtggacgtta cggctgcctg gaaaccgtcg
    29341 cctcgttaag tgcattaaaa aaacaggcgc gggtatggct aaaatcacaa ccggttaata
    29401 ctcagcttga tcctgaaaaa ctgacaaccg cgcagttaat cgctgcctgg cagagtggag
    29461 aaccgtggat caccagctgg gttgatcgct ctgccaatgc cattggtttg agtctgtata
    29521 acttcctcaa catcctcaat attaatcaga tttggttgta cggtcgcagt tgtgcctttg
    29581 gtgagaactg gcttaatact attattcgcc agacaggatt taacccgttc gaccgcgacg
    29641 aaggaccgag cgtgaaagcg acgcaaattg gcttcgggca attaagccgc gcacaacagg
    29701 tgctgggaat tggctatttg tatgttgagg cgcagttacg acagatttga tggcgcgata
    29761 acgtagaaag gcttcccgaa ggaagccttt tttcaacaga ttaaccgttt cgcttatgcg
    29821 taaaccgggt aacgtgcgca gatgtcgaga actttacctt tgatgcgctc gataacggct
    29881 tcatcattga tgctgtccag cacgtcacac atccagccag ccagttcttt cgcttctgct
    29941 tctttgaagc cgcgacgcgt aattgccgga gtacccacgc gaataccgga ggtcacaaac
    30001 gggctcttcg gatcgttcgg tacgctgttt ttgttgacgg tgatgttagc acggcccaga
    30061 gcggcgtctg cttctttacc ggtcaggttt ttatcaacca gatcaaccag gaacaggtgg
    30121 ttatcagtgc cgccggaaac cactttgtag ccgcgctcga ggaacacttc taccatcgct
    30181 ttagcgtttt tcgcgacctg ctgctggtaa gttttgaact caggctccat cgcttctttc
    30241 agagcaaccg ctttaccggc gattacgtgc atcaacggac cgccctgacc accagggaaa
    30301 acggcagagt tcagtttttt gtacagctct tcgctaccac ctttcgccag gatcaggccg
    30361 ccgcgcggac ccgccagggt tttgtgagtg gtggtagtaa caacgtgagc atgaggaacc
    30421 gggttcgggt agacgccagc agcaaccagg cccgcaacgt gcgccatatc aacgaacagg
    30481 taagcaccga tgctgtcagc gatttcacgc attttcgccc agtccaccac gccggaatat
    30541 gcagagaaac caccgataat cattttcggc ttgtgttctt tggcttgttt ttccagatcg
    30601 gcgtagtcga tatgaccggt agcatcgata ccgtaaggaa cgatgttgta cagtttaccg
    30661 gagaagttaa ccggagaacc gtgagtcagg tgaccgccat gcgccaggtt catacccaga
    30721 acggtatcac ctggttccag cagcgcggtg tagaccgcaa agttagcctg ggagccggag
    30781 tgcggctgga cgttagcgta gtcagcgccg aacagttctt tcgcacgatc gatcgccagt
    30841 tgttcaacga tatcaacata ctcgcaaccg ccgtagtagc gtttgcccgg ataaccttca
    30901 gcatatttgt tggtcagctg agaaccctgc gcctgcatta cgcgcgggct ggtgtagttt
    30961 tcggaggcga tcagttcgat gtgctcttcc tgacgtactt tttcctgctc catagcctgc
    31021 cacagttcgg catcataatc ggcaatgttc atttcacgct ttaacatccg catctcctga
    31081 ctcagctaac aataaaattt ttggccttta taggcggtcc tgttggacaa cggcgaacag
    31141 tataaccgaa tcattgtgcg ataacaggtc ttgacaaagg aatttacgca aacgattacc
    31201 ttcaggctac acaaggcttt ggagaataaa gagcttgcaa ccggaaacgg atttcttttc
    31261 aggtttgtga tgcaaatttt tcacttcatc acattctttc tgaaaaacac caaagaacca
    31321 tttacattgc agggctattt tttataagat gcatttgaga tacatcaatt aagatgcaaa
    31381 aaaaggaaga ccatatgctt gacgctcaaa ccatcgctac agtaaaagcc accatccctt
    31441 tactggtgga aacggggcca aagttaaccg cccatttcta cgaccgtatg tttactcata
    31501 acccagaact caaagaaatt tttaacatga gtaaccagcg taatggcgat caacgtgaag
    31561 ccctgtttaa cgctattgcc gcctacgcca gtaatattga aaacctgcct gcgctgctgc
    31621 cagcggtaga aaaaatcgcg cagaagcaca ccagtttcca gatcaaaccg gaacagtaca
    31681 acatcgtcgg cgaacacctg ttggcgacgc tggacgaaat gttcagcccg ggccaggaag
    31741 tgctggacgc gtggggtaaa gcctatggtg tactggctaa tgtatttatc aatcgcgagg
    31801 cggaaatcta taacgaaaac gccagcaaag ccggtggttg ggaaggtact cgcgatttcc
    31861 gcattgtggc taaaacaccg cgcagcgcgc ttatcaccag cttcgaactg gagccggtcg
    31921 acggtggcgc agtggcagaa taccgtccgg ggcaatatct cggcgtctgg ctgaagccgg
    31981 aaggtttccc gcatcaggaa attcgtcagt actctttgac tcgcaaaccg gatggcaaag
    32041 gctatcgtat tgcggtgaaa cgcgaagagg gtgggcaggt atccaactgg ttgcacaatc
    32101 acgccaatgt tggcgatgtc gtgaaactgg tcgctccggc aggtgatttc tttatggctg
    32161 tcgcagatga cacaccagtg acgttaatct ctgccggtgt tggtcaaacg ccaatgctgg
    32221 caatgctcga cacgctggca aaagcaggcc acacagcaca agtgaactgg ttccatgcgg
    32281 cagaaaatgg cgatgttcac gcctttgctg atgaagttaa ggaactgggg cagtcactgc
    32341 cgcgctttac cgcgcacacc tggtatcgtc agccgagcga agccgatcgc gctaaaggtc
    32401 agtttgatag cgaaggtctg atggatttga gcaaactgga aggtgcgttc agcgatccga
    32461 caatgcagtt ctatctctgc ggcccggttg gcttcatgca gtttaccgcg aaacagttag
    32521 tggatctggg cgtgaagcag gaaaacattc attacgaatg ctttggcccg cataaggtgc
    32581 tgtaatttga tgttgccgga tggaaacatc cggcaaccct tgacgcggtt taaattgccg
    32641 cgtcgtcctc ttcaccggta cggatgcgaa tgacccgtgc cacgtcgaag acgaagattt
    32701 taccgtcacc gattttgccg gtttgcgccg tgcgaataat ggtatcaaca caggtatcga
    32761 caatgtcgtc cggtacgaca atctcaattt tcactttcgg cagaaaatcc accatatact
    32821 ccgcgccgcg gtacagctcg gtatggcctt tctggcggcc aaaacctttc acctcagtga
    32881 ccgtcatgcc ggtaatgccg acttcagcca gtgcttcgcg aacatcgtcc agcttgaagg
    32941 gtttaataat cgcatcaatc tttttcatgc tattccttga aaaggtcgcc tgtcttttga
    33001 tctgctaaac gtaacacata acgccaattc attccttgaa atcgtttgca tccagctcgt
    33061 gtcgggaaag cagtttataa aattctgtcc ggttgcgccc cgccattctc gccgcgtgtg
    33121 tgacgttgcc tttggtgatt tgcagcagct tacgcaaata gttgagttca aactgattac
    33181 gtgcctcaac aaaggttggc agcgccgtat tttcaccctc cagcgcctgc tctactaatg
    33241 catcgctaat aaccggagat gaggtcagcg ccacgcactg ttcaatcacg ttgaccaact
    33301 ggcgcacatt acccggccag ctagcggtca tcaggcgttt catcgcatca gtggagaacg
    33361 cgcggacaaa cggtttatgt cgctctgccg cctggcgcaa caggtgattt gccagaagcg
    33421 gaatgtcttc tgtgcgctct gccagagccg gaattttcag gctgacaacg ttgaggcggt
    33481 aatagaggtc ttcacggaat tccccgcgcg ccatcgcttt tggcaggtcg cggtgagtgg
    33541 cggaaataat ccgcacgtcg atatcaatat cgcggttact gcccagcggg cgcactttac
    33601 gctcctgcaa cacgcgcagc agtttgacct gcaacggtgc gggcatatcg ccaatctcat
    33661 cgagaaatag cgtaccgcct tccgccgcct ggaataaacc ttcgcgattg ctgacagcgc
    33721 cagtaaacgc gccacgcgca tgaccaaaca gctccgactc cagcaattgc tcgggcaatg
    33781 cgccacagtt aatagcaata aatggtttgc tattgcgcgg gctggcgttg tggatagcct
    33841 gggcgaaaat ctctttcccg gtgccgctct ggccgttaat caaaacgcta acgtctgatt
    33901 gcgccaccag ccgcgcctgt tccagcaaac gcagcatcag cgggctgcgg gtgacaatcg
    33961 cctcgcgcca gcgttcatcg gtggctggcg cggattgttc cagcgcatcg tcaattgcct
    34021 gatatagcgc atctttatcg acaggcttgg tgaggaaact gaacacgccc tgctgtgttg
    34081 cagcaacggc atcgggaata gaaccatgcg cggtaagaat aattactggc attcccggct
    34141 gcactttctg aatttcagca aacagctgca taccgtccat ttcatccatc cgcaggtcgc
    34201 tgatgactaa atctactttt tcgcgattca gtacccgtaa tccttcagcg ccactttccg
    34261 ccgtgaccac gctgtagcct tcgctggtca ggcgcaggcc aagcagtttc agcaatcccg
    34321 gatcgtcatc gaccaataat aaatgcgcag gtttatggct catcaggagt gacctcatgg
    34381 gtggatggcg cgggcttttc accctcgtgc ggcgtatccg gcgagaaatt tccagccggt
    34441 ttgcgggtcg agagctggcg ttcaatatca gtcaggtttt ccagcttgcg ggtggtgagt
    34501 tccagttgct gctgtagaac gtgatgttgc tggcgcaatg tatccagctc gctgtcgctg
    34561 gcctgctgga gcttgccgta gcgttggcgc tcttccgcca gttgcagttg cagcgcctga
    34621 ccgtcgcgcc agagttgata cagagggcga acctgtgccg ggatctcggt acttagcgca
    34681 tcaatgcgcg cgaccagctg gcggcgctca tacggcgtaa ttttggcgtc ggcgagcaaa
    34741 atcccttgtt taaaggtatt ttgccagctg ccgtcgtcat attggcgagc ttgctgacgt
    34801 gactgcgcag gcattaaacg atcagcacaa tccatcgccc gcagccagta aagcggattg
    34861 gtttcggttg atttaccttg cagcgcccag atgtcgctac attcagtaga aagatagtca
    34921 gccagttgat aaaccggaat tttttcttct gctggcgtat caatggctgg cttattgtga
    34981 ctctgcacgc aacccagcaa tgccagacat ggcagccctg ccagccacag ccgtcggggc
    35041 attaatcgtt gaaaaatgtg tcgcatattc accagactta atgcctatcc cagtgggcgt
    35101 aattattgca ggcagtttgg acatggacag cgcggagaaa ccggagcgta cacgtcgtac
    35161 gtgaggattt cgagcactgc ccggggccga actggcaaat aaaatagcct gatgggatag
    35221 gctgttagca ttatttcgtg tttttcgacg acggcaattc aatgcggaaa caaacgtctt
    35281 gcccgctctc gtcgaccaga tacagttccc cttgcatacg gcgaatacaa tccctggcaa
    35341 tgcttaatcc cagaccgctg cccttcaccg ccccttttcg ctggtggctt ccttgaaaaa
    35401 agggttcgaa gatcatggcg cgttcctctt gcggaatggg tgtgcctgta ttgatgacat
    35461 caatataaac ccgtgcacca tgtaagctgc tgcgaaggca aatgttaccg gattcagccc
    35521 cgtagtgcac cgcattggag taaagattat ccagtacgct catcagcagc attggctccg
    35581 ccaggcaagc tgttgctttg agatcgacgt cggtatgcat cattttagcc cgtgcgggca
    35641 ggctatgagc agaaactact gtctccacca gcggtgctaa ctcaacattc tccagttcca
    35701 ccgcactgtc cgcctgttta cggttgtaat caagcagttg ttcgatcagt ttttgcaaat
    35761 tgcggctgct gctatcaaga atgctcacca cctctttttg ctctggcgta agcggcccga
    35821 caacctggtc agccagtaat tcagtgccct cgcgcatact cgccagtggc gtttttaatt
    35881 catgagataa atgtcttaaa aattgatggc gttgggattc cagccatgac aggcgctcac
    35941 ttaaccaaag aatacgttgc ccaaccgagc gtaactcgct cggtccactg aacgaaacgc
    36001 tattgcccag agaacgccct tcccccagac ggttgatcat gcgctcgata tttttcaccg
    36061 gcccgataat catccgcgtg aaaagtagca ccatcaccag actcaccaga aatagcacca
    36121 gcgattgcca accaaaatat tgcccacgtt cggcgatttc acgctgaagt tgctgcccac
    36181 gagagaacac cactgtgcgc gtggcctgca ccatttcggt attggcactg gcaaaggctt
    36241 caagacgcgc ggcggcggca gcatcgggac cactgttgtt acactgaagt tgggccagat
    36301 tgttcaaatc ctgacgcaat gcctggtaaa gtttatcgtc tggcagtacg cctgcgtggg
    36361 catcgagcat ttcgctgtaa cgcttgcgct ggctttgata aaccttcgca agcgtagggt
    36421 cgtccagcac gcaatactga cggtagctac gctccatctc cagcgccgcg ttggtcatcg
    36481 cttcactgcg ccgggcgtca ataagcgtgg tgcggttaac cagcgccgcc tgatcgctaa
    36541 gcgcattcag gctttgccag gcttgccatg ccagcaccaa caagggcagc agaattagca
    36601 aaaatgccag cattaccagt tgtcgtaatg agcggggaaa aacgggccag cgtttcaagg
    36661 tgttactctc gtcagacgcg aatagcctga tgctaaccga ggggaagttc agatacaaca
    36721 aagccgggaa ttacccggct ttgttatgga ataaggcggt gcctaactcg acgtttcgcc
    36781 cgatggttga tatagctacg ctgatatcag aagttggacg gcaggcacct tgttgtgcgt
    36841 cattcgaatt ttatgtagca cgtcccgaag gggctgacat aagtcggtga atgagccact
    36901 gcttactatt atgcagtaac tgtgccaata aagaaaatag tttggtaacg tactgattat
    36961 acgttgcttt gagggattta tggcttcttc gctatttgaa tgtaaatcag ccactgtgtc
    37021 gctaaaaaga gacaacttaa gataaactta ttagattaca ataaaatcaa tcatttaagt
    37081 gtcgccagaa agcgacacgg taaaccaccc attgtcgtga tttacagaca caaaaaagct
    37141 cccggagttg ggagcttatg atagtggttg gtgcttaatg ccgcatccgg gctgcaaaac
    37201 caatgaactg atgaattacc ccaactgctt acgcgcattg cggaaaatgc gcatccatgg
    37261 gccatcctcg ccccagtttt ccggatgcca ggagttgctg acagtacgga aaacacgttc
    37321 cgggtgcggc atcataatgg tgactcgacc actttcagtc gtgactgccg taataccgtt
    37381 cggtgaaccg ttcgggttag ccgggtaggt ttcagtgact ttgccgaagt tatcgacata
    37441 gcgcagtgcc accagcactt tgctttccag tgccgccaga tgcgccgcat cacgcacttc
    37501 cacgcgccct tcaccatgag agacagcaat cggcatttgc gagcccacca tcccctgcaa
    37561 cagcagagac gggctttggg ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga
    37621 ggtattgcgc acaaaacgtg gccacaactc actacctggg atcagttcgc gcaggttaga
    37681 catcatctgg caaccgttac atacccccag cgccagcgtt tgcggacggt ggaagaaggt
    37741 tgcaaactca tcgcgtacac ggtcattgaa caggattgac ttcgcccatc cttcaccggc
    37801 acccagcaca tcaccgtagg agaaaccacc gcacgcgacc agggcgtgga aatcttccag
    37861 gcccgtgcgt ccagccagca ggtcgctcat atgcacgtcg atagcatcaa agcctgcacg
    37921 gtggaaagct gccgccattt caacatgcga gttcacgccc tgctcacgca gtacggcaac
    37981 tttcggacgt gcgccagtgg caatatacgg tgctgccaca tcttcgttga tatcgaacga
    38041 cagttttaca ttcaggcccg gatcggcgtc gttagatttc gcctgatgct cctgatcggc
    38101 acactccggg ttgtcacgca ggcgctgcat ctgccaggta gtttctgccc accagacacg
    38161 caacgtggtg cggctttcgc tgaatacagt ctgcccgttg gcggtaatca caaaacggtc
    38221 accggaaaca gcctgcccta cataatggac acaatcagca agcccatgct gtgccagtac
    38281 ggactcgacc gcttcacggt cagcggcacg aacctgaatc accgcaccca gttcttcgtt
    38341 aaacaacgcc gccaggcgat cgtcgcccag agaggcaata tccgcgttaa tgccgcaatg
    38401 accagcaaag gccatttccg ccagcgttac cagcaggccg ccatcagagc ggtcgtggta
    38461 tgcgagcagc ttacgctgtg caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg
    38521 cgcgacgtcg cgtacatctg ccggtttgtc gccaagctga cgataaacct gcgccagcgc
    38581 cgttgcgccc agcgcgttat tgcctttgcc caaatcaatc agcagcagtg cgttatcttc
    38641 ggtagaaagc tgtggcgtga tggtgtgacg tacatcttcc acgcgggcaa atgcggaaat
    38701 caccagcgac agcggcgacg tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt
    38761 catcgacata gagtctttac ccaccgggat cgtaagaccc agcgccggac aaagctcttc
    38821 acccacggct ttaacggctt catacaggcc cgcatcttca ccagggtggc ctgccgccgc
    38881 catccagttg gcggaaagtt taatgcgttt gatatcacca atctgtgttg cggcaatatt
    38941 ggttaacgct tcaccgaccg ccagacgggc agaggcggcg aaatccagca gcgcaaccgg
    39001 cgcacgctcg ccaatcgcca tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac
    39061 cgcgcagtta gcgaccggca cctgccacgg ccccaccatc tgatcgcgcg ctaccatgcc
    39121 ggttacgctg cggtcgccaa tggtcaccag gaaggttttt tccgccacag tcggcagatg
    39181 cagcacacgt ttcaccgcat ctgcaatggt gatcccttca cgggccagcg cgtcgccttt
    39241 cgctttcagc gtttgtacat cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag
    39301 cggcagatcg atcggctgat tatcaaaatg acgatcgtgc agagaaagat gcagttcttc
    39361 ggtcgcttca ccaatcaccg cgtagggtgc gcgttcacgc ttacacagtt cgtcaaacag
    39421 cggtaattga tcggcagcaa ccgccagcac gtagcgttcc tgggattcgt tacaccagat
    39481 ttccagcggg ctcatgcccg gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc
    39541 gcgtccgccg tcgctcacca gctccggcat cgcgttagaa agaccgcccg caccgacgtc
    39601 gtggataaac agaattgggt tagcatcacc aagctgccag caacggtcga tcacttcctg
    39661 gcagcgacgc tccatctccg ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc
    39721 agactgacct gacgccatgg aagacgccgc tccgccgcca agaccgatgt tcattgccgg
    39781 gccgccgaga acgaccagct tcgcaccgac gttgatctcg cctttttgta cgtgatcggc
    39841 gcgaatgttg ccgatcccgc ccgccagcat gatcggtttg tgataaccgc gcagctcttc
    39901 gccgttgtgg ctgttcactt tttcttcata agtacggaag tagccattca gtgccggacg
    39961 accaaattcg ttgttaaacg ccgcgccgcc cagcgggcct tcggtcatga tgtccagcgc
    40021 ggtgacaatg cgctcaggct taccgaaatc ttcttcccac ggctgttcga agccaggaat
    40081 tcgcaggttg gaaacggaga aaccaaccaa acccgctttc ggcttcgcgc cgcgcccggt
    40141 ggcaccttca tcgcggattt caccgccgga accggtcgcc gcgcccggcc acggagaaat
    40201 cgccgtcggg tggttgtgag tttcgacttt catcagaata tgcgccggtt cctgatggaa
    40261 atcgtagcgg cccgtttcgt ggtcagcaaa gtagcggccc acttcagaac cttccattac
    40321 ggcggcgtta tctttataag cagagagaac gtaatctggc gtggtttcga aggtgttttt
    40381 gatcatctta aacagcgatt tcggctgttg ctcaccgtcg ataacccaat cggcgttaaa
    40441 aatcttgtgg cggcagtgct cggagttcgc ctgggcgaac atatacagct cgatgtcgtt
    40501 cgggttacga ccaagctttg tgaaagcatc ctgcagatag tcaatttcat cttccgccag
    40561 tgccaggcca agacgcaggt tagcgtcgat tagcgcctga cggccctgcc ccagcaaatc
    40621 aacgctggtg accggagtcg gttgatggtg tgcaaacagc tgctctgcat catctaaagc
    40681 aaaaaagacc gtttccatca tgcggtcgtg cagttcagcg gtaacctgct gccattgttc
    40741 attggtcagc gtaccggctt ctatatagta agcaacgccg cgctcaaggc ggtttacctg
    40801 ttgtagcccg cagttatggg caatatcggt cgctttcgaa gaccagggag agatggtgcc
    40861 aggacgcggg gtcaccagca ggagtttgcc ttgcggggcg tggctggcga gtgccgggcc
    40921 atatttcagc aggcgttcaa gttgtgcgtg ctcatcatcg tttaacggcg cattgaggtc
    40981 agcaaaatgg acatactcgg cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg
    41041 tgccagcagt ttgttgattc ggaatgccga cagtgcaggc gaaccacgca gaatttccat
    41101 cataagtctc tcgtcttcta agctttcggt gtacccaagg gggggaaacg ggcgtcatta
    41161 taaagaatct gatgcgctga cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt
    41221 agcaacgcgt gccgccaatg gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat
    41281 gccgctcatt tatgagtaaa cctttcacta ttattacgtt ttttcaagct gggacgcgca
    41341 cgacacagag aattaactaa ttgaaaaaat taaagattaa ttatctgttc atcggcattc
    41401 tggcactgct gctcgcggtc gctctctggc catccattcc ctggtttggt aaagccgaca
    41461 accgtatcgc cgccattcaa gcgcggggag agttgcgtgt gagcaccatt catactcccc
    41521 tgacttataa cgaaatcaac gggaaacctt ttggcctgga ttacgaactg gcgaaacagt
    41581 ttgccgatta cctcggcgta aaactgaaag tgaccgtgcg gcagaatatc agccagctgt
    41641 ttgacgacct tgataatggt aacgccgacc tgctggcggc aggactggtc tataacagtg
    41701 agcgggtaaa aaattatcag cctggcccta cctattattc cgtgtcacaa caactggttt
    41761 ataaagtggg tcagtatcgc ccacgtacgc tgggcaacct gacggcggag caactcaccg
    41821 ttgcaccggg tcatgtggtg gttaacgatc tccagaccct gaaagaaaca aaattcccgg
    41881 aattaagctg gaaggtagac gacaaaaaag gctctgcgga attaatggaa gatgtcatcg
    41941 aaggaaaact cgattacacc attgctaatt ctgtcgccat cagcctgttt cagcgcgttc
    42001 acccggagct cgccgtagcg ctcgatatca ccgatgaaca accggtgact tggtttagcc
    42061 cgttagatgg cgataatacc ctttccgccg ccctgctcga cttcttcaac gaaatgaatg
    42121 aagacggtac gctggcacgc attgaagaga aatacctggg gcatggcgat gattttgatt
    42181 acgtcgatac gcgcacattt ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc
    42241 tgtttgagaa atacgccgaa gaaattgact ggcgtttgct ggccgctatt gcttatcagg
    42301 aatcgcactg ggatgcacag gccacttcac cgacgggtgt gcgcggcatg atgatgttaa
    42361 ccaaaaatac cgcgcaaagc ctcggcatta cggatcgtac cgatgccgaa cagagcatca
    42421 gcggtggcgt gcgttatttg caggatatga tgagtaaagt gccggaaagt gtgccggaga
    42481 acgagcggat ctggtttgcc ctcgctgcgt acaatatggg ctatgcgcat atgctggatg
    42541 cccgcgccct gacggcaaaa accaaaggga atcctgacag ttgggctgac gtaaaacagc
    42601 gtctgccttt acttagccag aaaccctatt acagcaagct gacttacggc tacgctcgtg
    42661 gacatgaagc ctacgcttat gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt
    42721 atctgcaaga gaaagagaag caggctacag aagcggcgat gcaactggcg caggattatc
    42781 cggcggtatc gcctacggag ttgggcaaag agaaatttcc ttttctctcg tttctttccc
    42841 agtcgtcatc aaactatttg acccattctc cctctctgct gttttccagg aaagggagtg
    42901 aagagaaaca aaattaatcc gtcgaggatt gcgctttttt ctgcgcttta atttcctggc
    42961 ggcgcatgcg aaagaagtca ctgagcaacg ccgcgcactc atccgccagt attccttccg
    43021 taatttccac tcggtgattc atacccggat gatgcagcac atccattaaa gatcccgcag
    43081 cgccagtttt cgcgtcacgc gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg
    43141 ctccggcaca cattacacat ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat
    43201 aattttgcat caccagacca ccctgccgca gggccatgat ttctgcatgt gcggtgggat
    43261 catggcgacc aatcgggcgg ttccagcctt cgccgattac ccgattgtta tgcactaata
    43321 ccgcgccgac cggcacttcc cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac
    43381 gcatccagta ttcgtggcta aattcgactt cagacaaaaa gaaaactccg gttataaaag
    43441 cgcggcgcat tatacacgga cgctatgctt tactctattc cagttgctgg agttcaccgc
    43501 gcggggttac tcgccaacga tgctgacaga aataaagcag cggattgtcc tgattactgt
    43561 cgctatagcc actgtacagc cgcagcggag tgccgatttt gcgctccagt tgtgcgacct
    43621 tttcatgtcc cagacaacgc atcgtcaata cccaaccacc atagccacgc tgaatttggc
    43681 tggcgataag attaacccgc ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg
    43741 gctgcggaga gccggtaatc aaccagatat cagcatcgga acttaacagg taggtggtta
    43801 atcgctcctg aaccagcgga aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg
    43861 cctgcaacgt ctgtaaacgt gcttcgctgt gaccaaaagt gcacccccac agaagcagac
    43921 tcatcggcca gcgtgccgca cgacctttta tcaataacgc aatggctata atcggtaaca
    43981 acggcaggac aagtaacgca ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc
    44041 cgaacatatc ctgctgatgc aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt
    44101 gagttgccaa accatactcc tctggactga aacatcctga ttaattttcg ctactacata
    44161 gcctaacaga tagatcatca cttttccggc aacaatcgac cgcatcatgg ctaactggaa
    44221 tttttaattc atggcaatta gcggcaatgg aatataaaat tcactcgcgt gtctctcata
    44281 ttgcctgttg tcgccactat ttcatcgcga ggaaagatat gaacggctta cttcgtatcc
    44341 gtcagcgtta ccaggggctt gcccagagtg ataaaaaact ggcggattat ctgctgctac
    44401 aacctgatac ggcgcgccat ttaagctctc agcaactggc caacgaagcc ggagtcagtc
    44461 agtccagcgt cgtgaagttc gcgcaaaaac tcggctataa aggttttccg gcgcttaagt
    44521 tggcgttgag tgaagcgctg gcaagccagc cggaatcgcc ctccgtgccc attcataacc
    44581 aaatccgcgg tgatgatccg ttacggctgg tcggcgaaaa actgattaaa gaaaatactg
    44641 ccgctatgta cgcaacgcta aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa
    44701 tgttgcgctc tgcgcggcgg ataattctga ccggtattgg cgcttcgggt ctggtggcgc
    44761 aaaactttgc ctggaagctg atgaagattg gcttcaatgc tgccgcagtg cgcgatatgc
    44821 atgcgctact cgcaacagta caggcgtcgt cccctgacga tctgttatta gccatttcct
    44881 acaccggtgt acgacgcgag ttaaacctgg cggcagatga gatgctgcga gtgggcggaa
    44941 aagtgctggc gattaccggc tttactccga atgccctgca acagcgtgct tctcattgcc
    45001 tgtataccat tgccgaagaa caagcgacaa acagcgcttc aatctctgct tgtcacgctc
    45061 agggaatgtt aacggatttg ctgttcattg cgctgattca gcaggatctg gaactggcac
    45121 cagaacgtat tcgtcatagt gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc
    45181 gtataatgcc cgcccggttt gtgttgtttt gagagtttcc ttatggcgtt gttaatcact
    45241 aaaaaatgca tcaattgtga tatgtgtgaa cccgaatgcc cgaatgaggc gatttcaatg
    45301 ggagatcata tctacgagat taacagcgat aagtgtaccg aatgcgtagg gcactacgag
    45361 acaccaacct gccagaaggt gtgcccgatc cccaatacta ttgtgaaaga tccggcgcat
    45421 gtcgagacag aagaacagtt gtgggataaa tttgtgctga tgcaccacgc ggataaaatt
    45481 taatattcta ctctggaagt agagtattaa ttatattact gggaagcctt aacgccatta
    45541 tatttattta attgatgaca ttagcataat cattcactaa gttaatttat atagtatctg
    45601 ccaagacact tatttatagt tattaaaggc gcgtccgatt ggttcaccgg acgcacctta
    45661 agtacgtttc cttgtgttat aagaacagaa ggatcagctg taaaacagca atgatgattt
    45721 tgatgacccg tttaatcagg tatcggcaat cagtcattcg tttttcctta aacaaggaat
    45781 gcagccgttc agattagccc ttacatctcc ccaaaactga acgtgcgagt tattgagggt
    45841 gcatgctgca ctccacacca gagctttgac gacaccactc gtttcaatgg gggaattctg
    45901 tggcatggtg taaagcacag caaaatcttc aataacgaag ccaattttaa tgtacttacg
    45961 aattggcggt cacgttaatc tttccatcaa tattgctttc ttcgtaaagg ctcgagtttt
    46021 tatgctaaag attgcaagtt gcttgtaaaa gataagtaca ctgatccata atcgctgttg
    46081 ttgagggtgc atgctgcaca aaattaaagt taaaaagtaa aacccccgtt ccttaccagt
    46141 tcgggggttt tactttttaa agagaacggt attattttaa ctttcaataa ttaccgtggc
    46201 acaagcatag tggcgttcat ctgccagcgt tacatgcata tttacaacgc cgagcttttc
    46261 cgccagtttt aatgcctcac cccatagccg tagccgtggt ttgccgagct catcattgaa
    46321 tacttcaaat tgattaaacg ccagaccatt gcggatcccg gtgccaaacg cttttgctgc
    46381 ggcttctttc acagcaaaac gcttcgccag aaaacgcacc ggctggtggt gcgttttcca
    46441 gattgcccat tcgttatcgc ttaatacgcg gcgtgccagg cgctcaccgg atcgggcgat
    46501 cactgcttct atgcgagcga tctctacgat atccgtgcct aaacctaata ttgccattag
    46561 ccacgcgctt ccagcatcag acgcttcatt tctgccaccg catctttcag tccggtcatc
    46621 actgcacgac caataatggc atgaccgata ttcagttcat gcatctcagg gatggcggca
    46681 atagctttca cgttgtgata ggtcagaccg tgaccagcgt taactttcag accgagacta
    46741 gcggcaaagg tcgcggcttt ggcgatacgc gccagctctt gcgcctgttc ggcgtcagtt
    46801 ttggcatcag catagcaacc ggtgtggatc tcgataaacg gtgcgccaac ctctgccgca
    46861 gctttgatct gctcttcatc ggcgtcgata aagagagaaa cctggatccc ggcatctgcc
    46921 agacgtttgc aggcatcgcg cattttgtca cgctgccctg cgacgtccag gccgccttcg
    46981 gttgttactt cctgacgctt ttccggtacc agacagcaaa aatgtggctt cgtctcaacg
    47041 gcgatcgcca gcatctcttc ggtcaccgcc atctccagat tcatgcgggt atccagcgtc
    47101 tgacgcagga tgcgcacatc acggtcagta atgtggcggc gatcttcacg taaatgcacg
    47161 gtaatgccgt ccgctcccgc ctgctcagca ataaacgccg cctgcaccgg atccgggtaa
    47221 gcagtaccgc gtgcgttacg cagcgttgca atatggtcaa tgttgacgcc taacagtaat
    47281 tcagccatga caatcctcat cattcataat gtgttttcac cgttcgctta ggcataaact
    47341 gccggaacag ttccctgctc tttaatggtt taccgccaag atacggctta agcgccatgc
    47401 gggtaaagcg tttcgcggcg cgtagtgtgt ctgcgtcagg aaattcccgc gcgtttaacg
    47461 cttttaactg ccttccggtg aacgttttat tgtcgataac gacgcttgcg ataaaccctt
    47521 tttcttcgcg ataacgatac gtcatggtgt catctaccgg ctcgccgcta cccgcacaat
    47581 gggtaaaatt gacgccatag cccagatgcc cgagcagtgc caattcaaag cggcgcagcg
    47641 cgggttctgg cgtaccagtg gcacctgcaa gagactgaat gcagtgcaag taatcgaaaa
    47701 agagttcaga gaagcgcgtc tcgtattcca gtacgcggga gagaagttcg ttgatgtaca
    47761 gaccgctgta aagcgtgata ccgcttaatg gcagcgccag cgagacagct tcagcactgc
    47821 gcagcgtttt gacttcgcca cgcccgccga aacgtagcaa gagaggggtg aaaggctgta
    47881 atgcaccttt cagagtagag cgtttagagc gtgcgccttt ggcaaccaga cgcacgcgac
    47941 ccgattcctc cgtgaagacg tccagcatca ggctggtttc gctccacggg cgactatgca
    48001 ggacaaatgc gcgctgccag ccttccatcg gagtgactct taaagatcgt caacgtaacc
    48061 gagactgcgc agtgcgcgtt cgtcgtcggc ccaaccggat ttcactttta cccacagttc
    48121 aaggtggaca ggcgcttcga acatttcctg catgtcttta cgcgcttcaa tcccgatggt
    48181 tttgatcttg gcccctttgt tgccaataac catcttcttc tgcccttcac gctcaacgag
    48241 aatcaaacca ttgatgtcat aaccaccgcg ttcgttagag acgaaacgtt cgatctccac
    48301 ggtcacggag tacggcagtt cagcgccgag gaaacgcatc agtttttcgc ggatgatttc
    48361 agacgccata aaacgctgtg agcgatcggt gatgtaatct tccgggaagt gatgggtcgc
    48421 ttcagggagg tgcttacgca cgattgctgc gatagtgtcg acattcagcc cggtttcagc
    48481 agagatcggc acgatatcga ggaagttcat ctgacttgcc aggaactgta agtgcggcag
    48541 cagatcggct ttttcctgca cgttatccac tttgttcacc gcgaggatca ccggcgcttt
    48601 gccatcgcgc agtttgttga gcaccatttc gtcgtccggc gtccagcggg tgccttcaac
    48661 gacaaaaatc accagctcaa cgtcgccaat agagctgctc gccgctttgt tcatcaggcg
    48721 gttaatagcg cgtttttctt ccatatgcag gccaggggta tcaacgtaga tcgcctgata
    48781 cgcgcctcca gtatggatcc ccacaatgcg gtgacgagtt gtttgcgctt tgcgggaggt
    48841 gatggagatc ttctgcccca gcagtttgtt caacaatgtg gatttgccaa cgttcggacg
    48901 tccgacgatg gcaataaatc cgcagtaact tttatcgatg ctcattccag ctccagtttt
    48961 ttcaacgcct gttcggcggc agcctgctca gccttacgac ggcttgaacc tgtgccaacc
    49021 accggttcac tcaggccgct gacctggcag tggatagtaa attcctgatc gtgcgcttcg
    49081 ccacgtacct ggactaccag ataagtcggc agcggcagat ggcgaccctg caaatattct
    49141 tgcaagcgcg ttttcggatc tttttgttta tcgcctgggc taatttcgtc caaacgagtt
    49201 tgataccagt tgaggattaa tttctcgacg gtttgaatat cactgtcgag gaatacgcca
    49261 ccaattaatg cttcgacggt gtcggcgaga attgactcac gacgaaatcc accgctttta
    49321 agttcacctg gccctaaacg taagcactcg cctaactcaa attcgcgcgc cagttccgcc
    49381 agcgtattgc cacggaccag cgtggcgcgc atccggctca tatcgccttc atccacacga
    49441 gggaaacggt gataaagcgc attggcgata acgtagctca gaatagagtc gcctaaaaat
    49501 tctaaacgct cgttatgttt actgctggca ctacgatgag ttaatgcctg ctgcaacagt
    49561 tcctgatgat taaaagtgta gcccagcttc cgttgaagcc gattaattac gatggggttc
    49621 atgcgatacc aataaataaa tgcgtcaaca attcagcaca cgaaacagac ctgatataca
    49681 tggctctgct aactgcttcg ctgcagtttc agtttaccgg tatatgggga ccaacgctgt
    49741 ttcgtgtgcc gtggcaacct ggaggtgcca accttaaact tcgggggaat attctataca
    49801 caacgacggg ggatgtcgtt agccacggga gatttatctc ataaataatt cacgttgtcg
    49861 ccataacggc gacaacgtga acgaagatga gctattaatg gatgccgcca atgcgactta
    49921 agcgcacacc agtcggccat tcgccttctt gcttatcgaa gctcatccag atagccgttg
    49981 cccgaccgac cagattcgct tcaggcacaa acccccaata acggctgtcc gcgctgttgt
    50041 cgcggttgtc gcccatcatg aagtattgtc ccggaggaac aatccaggtt gccagttgtt
    50101 gccctggctg ctggtaatac atccccacct gatcctgcgc aatcggcact gtcagaattc
    50161 ggtgcgtcac atcacccagt gtctctttac gctcggaaag acgaattcca ttttctttgg
    50221 tttcgttttt cggcacttca aagaatccgc tggtcgcttc cccaccatta cggcgtgaga
    50281 aggtctgaac gaaatcgctc ggttccacgt ttgagtaggt gaccggcagc gcgttttcac
    50341 acgcctggcc ggaactgcat cccggttgaa tcgtcagctc tttcgagacc ggatcataag
    50401 taactttatc gcccggtaaa cccaccgcgc gcttgatgta atcaagcttt ggatcttccg
    50461 gatatttaaa gaccacgata tctccgcgtt tcggatgacc ggtttcgatc agcgttttct
    50521 ggtagatagg atctttaatg ccataagcaa acttttctac cagaataaaa tcaccgatta
    50581 acagagtcgg catcatcgaa cctgacggga tctggaacgg ttcataaata aacgaacgca
    50641 caatcaatac gatagccagt accggaaaaa cagaagcacc ggtttccagc cagccaggct
    50701 tcggcgcaac ctttttcaac gttgctttat ccagtgagtc accggcagcc gcctgcgccg
    50761 ctgcctgacg ttcccgccgt ttaggtgcga aaaagaattt atccacgcac cataaaatgc
    50821 ccgtcaccag tgtggcaatc accagaatca gggcaaacat attcgccatg ccaactccta
    50881 agggttattt gttgtctttg ccgacgtgca gaatggcgag gaacgcttcc tgcggcagct
    50941 cgacgttacc gatctgcttc atgcgtttct taccttcttt ctgcttctgc agcagctttt
    51001 tcttacggct gatatcgccg ccataacatt tagccagtac gtttttacgc agctgtttca
    51061 cggtggatcg cgcaataatg tgcgtaccaa tcgctgcctg aatggcgata tcaaactgct
    51121 ggcgtgggat cagatctttc atcttctcca ccaactcgcg accgcggttt tgcgaattat
    51181 cacggtgggt gatcagcgcc aacgcatcaa cacgttcacc gttgattaat acgtctacac
    51241 gtaccatgtc ggacgcctgg aagcgtttga agttgtaatc cagagacgca taaccacgcg
    51301 aggtagattt caggcgatcg aagaagtcga gcaccacttc cgccatcggg atctcgtacg
    51361 tcagcgccac ctgattaccg tggtaaacca tattggtctg cacgccgcgt ttttctacgc
    51421 acagcgtaat aacgttgccg agatatgcct gcggcagcag catgtgacat tcggcaatcg
    51481 gctcgcgcag ttcgtagatg ttatttaccg ctggcagctt ggatggactg tcgacgtaaa
    51541 taacttctct tgacgtggtt tcaacttcat acactacggt cggcgcagtg gtgatcagat
    51601 ccagatcgta ttcacgttcc agacgttcct ggatgatctc catgtgcagc aggccgagga
    51661 agccgcagcg gaaaccaaag cccagcgcgc tggagctttc cggctcatag aacagtgaag
    51721 catcgttcag gctgagttta cccagcgcgt cacggaaggc ttcatagtcg tcggaactta
    51781 ccgggaacag accggcgtat acctgcggtt tgactttctt aaagccaggc agcgcctttt
    51841 ctgccggatt acgcgccagc gttaaggtat cgccgactgg agcgccgtgg atatctttaa
    51901 tcgcacatac cagccagcct acttcgccac atttcagttc agtgcggtca acctgtttcg
    51961 gcgtgaagat gcccagacgg tcggcgttat aggtctgccc ggtactcatg actttcactt
    52021 tgtcgccctt acgcagggtg ccgtttttaa tacggataag tgaaacaacg cccaggtagt
    52081 tgtcgaacca tgagtcgata attagtgcct gcaacgggcc ttccggatcg ccttccggcg
    52141 gcggaatgtc gcgcaccaga cgttcgagaa cgtcctgcac gccaacgccg gttttcgctg
    52201 aacagcgcac cgcgtcggtg gcgtcgatgc cgacgatatc ttcaatttct tccgccacgc
    52261 gttcaggatc ggctgccggc aggtcaatct tgttcagtac cggcaccact tcgagatcca
    52321 tttccatggc ggtgtagcag tttgccaggg tttgcgcttc tacgccctgc ccggcgtcga
    52381 ccaccagcaa tgcaccttca caggcagcca gcgaacggga aacttcatag gagaagtcaa
    52441 cgtggcccgg ggtgtcgata aagttcagct gataggtttc gccgtcagac gctttgtagt
    52501 ccagcgtcac gctttgcgct ttgatggtta tgccacgctc acgctcaaga tccatggaat
    52561 cgagaacctg cgcctccatt tcacggtcag acaggccacc gcagatctgg ataatacggt
    52621 cagacagcgt cgatttaccg tggtcaatgt gagctatgat cgaaaagtta cgtatattct
    52681 tcataaagta tgattattgt gccttaatgc ccggttaacc aggcttttag aagtcgctgt
    52741 tctgagctta acgtctgtat taatagaaac gccgcattct acactacaac attgaggcga
    52801 ggaaatgttc ataccgtatg gattgtggta tctggaaacg tcctcgcatt tgttatgcaa
    52861 aatgcaacaa agccagtgaa atcactggct cgcgtcttcc gaagatgttt caaatcgcac
    52921 aaggccaggc ggcaatgcca cgcttaagat gatcggttgc cattctgccc gggcagcaaa
    52981 cttacgcgag tagccgcgcg caatcaggaa cccgccaata ccgccgagga ccgcaccaca
    53041 taatgctgcg acgtcagaag caaagagtag ctgaaatagc gaagcgatga ggaataatcc
    53101 caccagcggc gacatataaa ccagtaatgc ggagctaagc aggctgcctt cggcgatccc
    53161 caattccact ttttgccccg gcactaacgg ctcatcacag ggtacgacaa tggtatgcgt
    53221 ggtttgcggg ccaagtttat ttaacacgcg gctaccgcaa ccggcgcgtg aagcgcagct
    53281 gctgcatgaa gctttaacat cacaactgac cagcgcctgc ccgttttgcc aggagacgac
    53341 ggtagcccac tctttgatca ttgcgctgcc ccgaacttaa tattctcggc aatgcgtttc
    53401 gccgtttgcg gcggcagttc accgacaatg gtgatttcgg cgttatcacg tacgcttgta
    53461 ctgacggttc tgcgtccggt gcgcaacatc tgatcggtgc tcgatggcgt agcgcggtta
    53521 acgtttaccg agaagctgaa taatccgtcg gaatagagac gtgattcgat aggcatgttg
    53581 tccatcgtcg gtagcggacg tcgactactg gaaacttcgc taaaaccctg tggcaaccag
    53641 gttggcgtcc agctgaattt agctttttca cctacaggaa cagaaagcaa cggcggcaaa
    53701 tttgccttcg ccagcgtctg catactgctg ctgatatcct gattgacgtt aaaagcaatc
    53761 acgcgaaatt gttccagcgt ttcaccatcg cgatcaagga gatcaacccg catcggtaat
    53821 ttcgattcgg tgtccatcca cacgatgtag ctgtagcgtg taccatctcg ggcaaccacg
    53881 cgaatgactt cgcaaagacg atcagcaata cgcgtgcgcc cgacggagat aaagtcgtag
    53941 taaggagaaa ggtgtttgaa atcggtatag ataagcgatg gcagagaatc aacaatgtaa
    54001 tcgccattaa gcgtgaacgg ttcaagtccc ggttcaaaat agctgatttc attgccgcgc
    54061 tgtaccactt cccggcgcgg gccatccatt tgcaacaatt gtgcaagagg acggttatcg
    54121 aggcgtgcat gtcgataacg cagagactca acaccctgtt tattgatgct gatgaatgac
    54181 agctcgtaat tcagtgactg actggccagg ttcatctgct gtaataacgc cccggacgcg
    54241 ggagtggccg aggcgttagc agagaataac aggctacctg tcactaatga catggcaaac
    54301 caaagttgct tcattactgc gattgcgttc ctaaagtttg aattcctggc acctgtacag
    54361 cggcttgctg ggtttgcgcc tgctcaaact gaagctgttc agagtggagt cggcgttgca
    54421 gttcgtaatc ctgcaacatt gcattaatgc gacgacgctg ctcctgtacc tgctgctgtt
    54481 gaccattgtt tgcggtcgct tcagaaggta ctcccaggct taccgggctg gctttaccca
    54541 tcatcggcag tgtattaaat accggcgttt cgggctgctg ggacgtttca gattgtccat
    54601 tatagtgctg gacgccaacg ataactgcaa gcgatacgca tgctgctacg cccatttggg
    54661 taagctgtgc cgcccacgga cgtactttct gccagaatgg cattttctgc cattgatgcg
    54721 gcgcaggctg ggcttccggg atcaatgtcg ccggttgacg tactggctct tcttcaatgg
    54781 cggccatcac gcgtgaagag atatcgaaat ggagcacctc gggagtatca ccccgcattg
    54841 agtcacggat taagtgatag ctttcccagg ttttctgcat ttctgggtta tgagccagtt
    54901 cgttaagcag ctcactatcc agcgtttcgc catccattaa agcggaaagt tgttctttct
    54961 gcatgcctaa tacccttatc cagtatcccg ctatcgtcaa cgcctgataa gcggttgaac
    55021 tttgttatca atagcttccc tcgctcggaa gatacgtgaa cgcaccgtac ctaccggaca
    55081 atccatgata gcggctatct cttcatagct caggccatcc agctcccgca aggttattgc
    55141 catgcgcaaa tcttccggga gggactcaat agttcggaaa actatctgtc tcagttcttc
    55201 tgacaacatt aagttctcag ggttcgaaat ttctttcaac gcgccgccac tttcgaagtt
    55261 ttcagcttca atggcatcca catcactgga aggtggacga cgcccctgag caaccaggta
    55321 atttttcgct gtatttacag caatccgata cagccatgta taaaaagcgc tatctccccg
    55381 gaacgaatcc agcgcacgat aggctttaat aaaagcttct tgtaccacat cgggaacatc
    55441 acccgacggc acatagcggg aaaccagact cgccacttta tgctgatagc gcactaccag
    55501 taggttaaag gctttctgat ctcccttctg gacccgttca accaggacct ggtccgttaa
    55561 ctgctcgctc atccgaggta aagtctcccc aaaccaaatt tccacgcgct atcgaaacgc
    55621 cactccatta gctgcaattt gagcaagcaa agggttagag tgtctcgttt ttgtaaagtt
    55681 ccgtaacgca tctgtttttg tttgtcatgc tgtagacgga tcattatcta tcattataag
    55741 tctacagaat ctgaacatcg cattatctgt gtagaaatgc ccatttaact gcctgaagag
    55801 taacccaacg gcctttttat ttcaccacct aatcctccac cagccagtaa cttctctttt
    55861 tctcgccgcc ctgcgtcagc gtgtttagcc actgtaacaa atattaaaat agcaggtgtt
    55921 tattcgcaca acatgatgct atgctgacca aacagtgttt agtaaattaa acaaagaaaa
    55981 tgaatactct cccagaacat tcatgtgacg tgttgattat cggtagcggc gcagccggac
    56041 tttcactggc gctacgcctg gctgaccagc atcaggtcat cgttctaagt aaaggcccgg
    56101 taacggaagg ttcaacattt tatgcccagg gcggtattgc cgccgtgttt gatgaaactg
    56161 acagcattga ctcgcatgtg gaagacacat tgattgccgg ggctggtatt tgcgatcgcc
    56221 atgcagttga atttgtcgcc agcaatgcac gatcctgtgt gcaatggtta attgaccaag
    56281 gggttttgtt tgatacccac gttcaaccga atggcgaaga aagctaccat ctgacccgtg
    56341 aaggtggaca tagtcaccgt cgtattcttc atgccgccga cgccaccggt agagaagtac
    56401 aatccacgct ggtgagcaag gcgcagaacc atccgaatat tcgtgtgctg gagcgcagca
    56461 acgcagttga tctgattgtt tctgacaaaa ttggcctccc gggcacgcga cgggttgttg
    56521 gcgcgtgggt atggaaccga aataaagaaa cggtggaaac ctgccacgca aaagcagtgg
    56581 tactggcaac cggcggtgcg tcgaaggttt atcagtacac caccaatccg gatatttctt
    56641 ctggcgatgg cattgctatg gcgtggcgcg caggctgtcg ggttgccaat ctcgaattta
    56701 atcagttcca ccctaccgca ctgtatcacc cacaggcacg caatttcctg ttaacggaag
    56761 cactgcgcgg cgaaggcgct tatctcaagc gcccggatgg cacgcgtttt atgcccgatt
    56821 ttgatgagcg cggcgaactg gccccgcgcg atattgtcgc ccgcgccatt gaccatgaaa
    56881 tgaaacgcct cggcgcagat tgtatgttcc tcgacatcag ccataagccc gccgatttta
    56941 ttcgccagca tttcccgatg atttatgaaa aattgctcgg gctagggatt gatctcacac
    57001 aagaaccggt accgattgtg cctgctgcac attatacctg cggtggtgta atggttgatg
    57061 atcatgggcg tacggacgtc gagggcttgt atgccattgg cgaggtgagt tataccggct
    57121 tacacggcgc taaccgcatg gcctcgaatt cattgctgga gtgtctggtc tatggctggt
    57181 cggcggcaga agatatcacc agacgtatgc ctgatgccca cggcgtcagt acgttaccgc
    57241 cgtgggatga aagccgcgtt gagaaccctg acgaacgggt agtaattcag cataactggc
    57301 acgagctacg gctgtttatg tgggattacg ttggcattgt gcgcacaacg aagcgcctgg
    57361 aacgcgccct gcggcggata accatgctcc aacaagaaat agacgaatat tacgcccatt
    57421 tccgcgtctc aaataatttg ctggagctgc gtaatctggt acaggttgcc gagttgattg
    57481 ttcgctgcgc gatgatgcgc aaagaaagcc gggggctgca ttttacgctg gattacccgg
    57541 aactgctcac gcattccggt ccatcgatcc tttcccccgg caatcattac ataaacagat
    57601 aaaaagcctg ggtcagcgcc gtatacgctt cggaatagtt ctggtctggc ccacgaatga
    57661 ctaagcgatc gctaaagcat tctcccgcct gcggggagaa tgccagcagc acccgatgcg
    57721 gcagtcgcgc ttcgttttcc gccacatccg tccgcaaacg taaatgccag cccatgctta
    57781 atgccagttc agtaaaacca ttaccaatct gctctggcag caccacgcag aaaaatccct
    57841 cttcggtaat gcactccgcc gcacaggtca gcagcgatgg gtgatcaagc gtagtggtat
    57901 agcgagcctg ttcccgttga ggtgtcgcgc actctactcc ctgctgatag taaggtgggt
    57961 tactgatgat taaatcgaag cgtactgtct gctgtgtgat ccactgctga atatccgccg
    58021 tatggacgtt aatccgctct gcccacgggg actggttgat attttcctgc gcctgcgcgg
    58081 cagcttcact ttccagctca acggcatcaa ttatcacgct gtcactggtt cgctgcgcca
    58141 gcatcagtgc cagcaacccg ctacccgcgc cgatatcaag gcaacgtttc accccagcca
    58201 ccggtgccca tgcgcccagc aaaatgccat ccgttcccac tttcatcgca cagcgatcgt
    58261 gagcaacaaa aaactgttta aaagtaaatc cattacgacg aagcacggat gtagactgtg
    58321 acatgaaaat aaaaccttgc aggaaaaacg gcgatagcac cgggtgagaa caatacccga
    58381 gaagcgatat ccatacaaac agatgaagat tgcagccgta acgtctataa tcagcgcccc
    58441 acacagaggt agaacatgac tgtaacgact ttttccgaac ttgaactcga cgaaagcctg
    58501 ctggaagccc tccaggataa aggtttcact cgcccgaccg ccattcaggc tgccgccatt
    58561 ccgcctgcgc tcgatggccg tgatgtactc ggttctgcgc cgacaggcac cggtaaaacg
    58621 gcggcgtatc tgctgccagc gttgcagcac ctgctcgatt tcccgcgtaa gaaatccggt
    58681 ccgccgcgta ttttgatcct caccccaact cgcgagctgg cgatgcaggt ggccgatcat
    58741 gcccgcgaac tggcgaaaca tacgcatctg gatatcgcca ccatcaccgg cggcgtagcc
    58801 tatatgaacc acgcggaagt gttcagcgaa aatcaggaca tcgtggtcgc cacgaccgga
    58861 cgtctgctgc aatacataaa agaagagaac ttcgattgcc gcgcggttga aacgctgatc
    58921 ctcgacgaag cagaccgtat gctggatatg ggcttcgctc aggatatcga acatattgct
    58981 ggcgaaacgc gctggcgtaa acagaccctg ctcttttcgg caacgctgga aggcgatgcg
    59041 attcaggact ttgccgagcg tctgctggaa gatccggtgg aggtttctgc caatccgtcc
    59101 acccgtgagc gcaaaaaaat tcatcagtgg tattaccgcg ccgatgacct ggaacataaa
    59161 accgcattgc tggtgcatct gttaaaacag ccggaagcga cccgctcaat tgtgtttgtg
    59221 cgtaagcgtg aacgtgtgca tgagctggca aactggctgc gcgaagcggg catcaacaac
    59281 tgctatctcg aaggtgagat ggtacaaggc aagcgtaacg aagcgatcaa gcgtttgacc
    59341 gaaggtcgcg taaacgtact ggttgcaacc gatgttgccg cgcgcggtat cgacattcct
    59401 gacgtcagcc acgtctttaa cttcgatatg ccgcgcagtg gcgatactta tttgcaccgt
    59461 atcggacgta ccgcgcgcgc cggtcgtaaa ggcaccgcaa tttcgctggt ggaagcccat
    59521 gaccatttgc tgctgggcaa ggttggccgt tacattgaag agccgattaa agctcgcgtt
    59581 attgatgagt tacgcccgaa aacgcgtgcg ccaagcgaga agcagaccgg caagccatcg
    59641 aagaaagtac tggcaaaacg cgccgagaag aaaaaagcta aagagaaaga gaaaccgcgt
    59701 gtgaaaaaac gccatcgcga caccaaaaat attggtaagc gccgtaaacc aagcggaacg
    59761 ggcgtgccac cgcaaacgac agaagagtaa tctcaatgcc gggtttaagc ctggtattaa
    59821 aagtgctggc aaacgcaaaa ctgcctgatg cgctacgctt atcaggccta cgtggctcat
    59881 gcaatatatt gaatttgcac gatcttgtag gccggataag gcgttcacgc cgcatccggc
    59941 atgaataaag cgcactttgt cgacaagtta aatgccgggt ttaatcccga cattattttt
    60001 atccagccac aaaactctct tcaacacact gaataaaagt gtgcatcgcc gggctaaccg
    60061 cttttccagc atggtgtgcg cacatggccg taatggtctg cgactgttcg ccaaaaggca
    60121 attcaattaa ttctccgctc tcaagctctt tttccaccgc aaagcggggc aagtaactga
    60181 caccgatatt cgccgcgaca caacgtttga tactttcaat gcttaacagc tcaatggtgt
    60241 tttccaccgt gatccgccgc tgacgcagcg tgctctcaaa tatctgccgg aagacacatt
    60301 gcggttcgtt gataataaag ctacaggcgt tatgtcttcc cggctcagta aaatcgacat
    60361 ctgcaatttg cggtgaagcc accagcacca gcgattgttc ccccaactct cgtcggttca
    60421 gggcatcatc attccctacg cgataaaaga cgccgacatc ggcctcatca ttcagtagcg
    60481 catcacggat cacgtaacag ttcagcgact gcaacgataa acgcactttt ggtgcgcgtt
    60541 gccgaaaacg ctgcaacacc tgcggcattc ggtaagagag caacgtttcg cccgaaacaa
    60601 cgcgtagttc cccgtccgga tctgactcct ttttggcagc ctcacgaagc gtatccatca
    60661 ctcgggtaag ttcataaatg tgcggcagca acttttttcc ttcgcgggta aggcacatcc
    60721 gtcggccaat tttctcaaat aactggactg aaaactcctg ctcaagctgc tgaatatgaa
    60781 aagtcaccgt cgattgtgta cagcacaatt tttgcgaagc tcgcaaaaag gaaccctctt
    60841 ccaccacggt tttaagcgta ataaaacggc gcagatccat aaccccaaac ctatcgaaaa
    60901 tatcgaatct agaatataaa aacattcatt tttttaaatg ttccgtgtcg ggtactgtct
    60961 accaaaacag aggagataac aagtgacacc gaccctttta agtgcttttt ggacttacac
    61021 cctgattacc gctatgacgc caggaccgaa caatattctc gcccttagct ctgctacgtc
    61081 gcatggattt cgtcaaagta cccgcgtgct ggcagggatg agtctgggat ttttgattgt
    61141 gatgttactg tgtgcgggca tttcattttc actggcagtg attgacccgg cagcggtaca
    61201 ccttttgagt tgggcggggg cggcatatat tgtctggctg gcgtggaaaa tcgccaccag
    61261 cccaacaaag gaagacggac ttcaggcaaa accaatcagc ttttgggcca gctttgcttt
    61321 gcagtttgtg aacgtcaaaa tcattttgta cggtgttacg gcactgtcga cgtttgttct
    61381 gccgcaaaca caggcgctaa gctgggtagt tggcgtcagc gttttgctgg cgatgattgg
    61441 gacgtttggc aatgtgtgct gggcgctggc ggggcatctg tttcagcgat tgtttcgcca
    61501 gtatggtcgc cagttaaata tcgtgcttgc cctgttgctg gtctattgcg cggtacgcat
    61561 tttctattaa cgaaaaaaag cggaagaggt cgccctcttc cgcttagtaa cttgctactt
    61621 aagccttaca ggctttcagt aaaggtacga gcgataacgt cgcgctgctg ttccggagtc
    61681 agagagttaa agcgaactgc ataaccggat acacggatgg tcagctgcgg atatttttcc
    61741 ggatgcttaa ctgcatcttc cagagtttca cgacgcagaa cgttaacgtt caggtgttga
    61801 ccaccttcaa cgcgaacttc tggtttcact tctactggaa cttcacggta ttcaatgtca
    61861 cccagtttgc ttactgcaac cacttcatct tctgcataac ctgcttttgc aacgatgcaa
    61921 cgtgcttcgc ctttttcgct gtccagcagc cagaaagagt tcagcagatc gtcgttagcg
    61981 gctttagtaa tctggatacc tgtaatcatg tgatgcctcc ccggcaaaat tatttgattt
    62041 gttcagcctg tcgcggccaa ttggtaaaac cattgttgct tgagtgtata tatactcctc
    62101 aaccaccctt gattctttga tttaaatcaa taaaaaccac acatcgattg tggtcgtaaa
    62161 tggattttat tgttttacat caacttatgc gggtgtgaaa ttttaccaat ttacattttt
    62221 ttgcactcgt ttaagtctaa aaaatgagca tgattttgtt ctgtagaaag aagcagttaa
    62281 gctaggcgga ttgaagattc gcaggagagc gagatggcta acgaattaac ctggcatgac
    62341 gtgctggctg aagagaagca acaaccttat tttcttaata cccttcagac cgtcgccagc
    62401 gagcggcagt ccggcgtcac tatctatcca ccacaaaaag atgtctttaa cgcgttccgc
    62461 tttaccgagt tgggtgacgt taaagtggtg attctcggcc aggatcctta tcacggaccg
    62521 ggacaggcgc atggtctggc attttccgtt cgtcccggca ttgccattcc tccgtcatta
    62581 ttgaatatgt ataaagagct ggaaaatact attccgggct tcacccgccc taatcatggc
    62641 tatctcgaaa gctgggcgcg tcagggcgtt ctgctgctca atactgtgtt gacggttcgc
    62701 gcaggtcagg cgcattccca cgccagcctc ggttgggaga cgtttactga taaagtcatc
    62761 agcctgatta accagcaccg cgaaggcgtg gtgtttttgt tgtggggatc acatgcgcaa
    62821 aagaaagggg cgattataga taagcaacgc catcatgtac tgaaagcacc gcatccgtcg
    62881 ccgctttcgg cgcatcgtgg attctttggc tgcaaccatt ttgtgctggc aaatcagtgg
    62941 ctggaacaac gtggcgagac gccgattgac tggatgccag tattaccggc agagagtgag
    63001 taaatttgcg gtaaaatgcc ggatggcaga gttgccaccc ggctgattta tcaggcttta
    63061 ttctgacgcc accattcacc aagcaaaacg ccggttgcaa cagagatatt cagcccggca
    63121 acgttgcccg taccgtcaat cttcacgcgc agatcgttcg gatcgcgtgc ggcatccggt
    63181 aacccttcat attcctgacc cagcaccagt accattttcg ctggcagact ggttttgaac
    63241 agcggtttcc cctgctcgct ggaagtggtc actacggtgt aacctgcctg acggaaatca
    63301 tccaacacgt taacaatgtt gtcgccagta atcggctgaa cgtgctctgc gccgccttct
    63361 gcggtacgga tagccgcccc cgactccagc agtgccgcat cctgcaccac aacgcctttc
    63421 acgccgaagt gcgcgcagct gcgcatcatg ccacccaggt tatgcgggtt agattcgttt
    63481 tccagcgcca gtacacaatc ctgcgcgcct gcctggctta cccactgctg cacggttgtg
    63541 ccattacgct ttttgatcag gaagcaaacg ccgccgtgat gttccgtgcc tgacgctttt
    63601 gtcaattccg cttcatccac cacatggtac gctttgcggt ttgctgccat ccagcgcaag
    63661 gcttctttaa aacgcggcgt tacactctgg ataaaccagg cgcgaacaat cgcttccgga
    63721 cggctctgga aaagtgcctg acatgcattt tcgccgtaga cgcgggtttc ttccgcacgc
    63781 tgacgacgca acacttccgg atcaataaaa cttttaccac tgatgccacc gtgatcggcc
    63841 ttttccggcg tctcatcacc cggcgcgcgg gaaaccgtgc gccacggcga atcttcccac
    63901 ttgcggtcac ggggctgact ctgtttgtca tcgcgggcgg ggcgacggcc accgtcagca
    63961 cgagattttc ctggacgccc gccccctttc ccggtacgcg ggttgtgggt acgtttatca
    64021 gaatcatcat cactgcggac atacatcact ttgaccttgc cgcttttacc tttcatttca
    64081 tcgttcatgc ttttctccac cagcgctgcg cgaagcgcgc agattacccg aagtccgcgc
    64141 ggttcgccat gatttcgtac caaagcctgc gactatcata cctattgaat aaaacagatt
    64201 gttgtctgga acaatgtccc cgataatatg taacatatta gaaacatacc ggcgtcgttg
    64261 ccgataagtc tccttactca tcccgaggtt agttatgaat accgtttgta cccattgtca
    64321 ggccatcaat cgcattcccg acgatcggat cgaagatgcg gcaaaatgcg gacgctgcgg
    64381 tcacgacttg tttgacggag aggtgattaa tgcgaccggt gaaacactcg acaaattgct
    64441 gaaggatgat ctacctgtgg tgatcgactt ctgggcaccg tggtgcggcc cctgccgtaa
    64501 tttcgcacca atttttgaag atgtcgcgca agagcgtagc ggtaaagtgc gctttgtgaa
    64561 agtgaatacc gaagctgaac gtgaattgag cagtcgcttt ggaattcgta gtataccgac
    64621 gatcatgatt ttcaaaaacg gtcaggttgt cgacatgctt aatggcgcag taccgaaagc
    64681 gccgttcgat agctggctga acgaatctct ttaatcttac cggggcgcat cttgtgcccc
    64741 gttttctcct ctgcgacaat ggcgtttttt cgacgctctc ttatgaccga aaacgctgtt
    64801 ctccagttac gcgccgagcg tattgcgcgc gcaacacgtc cttttcttgc ccgcggtaat
    64861 cgcgttcgtc gctgccaacg ctgtcttttg ccagagaaat tatgtctctg ttcgacaatt
    64921 accccagcac aagcaaaaag tcgcttctgt ttgctgatgt tcgacaccga gccaatgaag
    64981 cccagtaata ccgggcgtct gattgctgat attttgcctg ataccgttgc gtttcaatgg
    65041 tcgcgtaccg aaccctcgca ggatttgctg gatctggtac aaaacccgga ttatcagcca
    65101 atggtggtct ttcccgcctc gtatgctgat gagcaacggg aagtgatctt cacaccacct
    65161 gccggtaagc caccgctgtt tatcatgctc gatggtacct ggccggaagc tcgcaagatg
    65221 tttcgtaaaa gtccgtatct ggataatctt cccgtcattt ccgtcgatct ttcccggctt
    65281 tctgcctatc gcctgcgtga agcccaggct gcaggccaat attgtactgc cgaggtagcc
    65341 atcgcactgt tagatatggc cggcgatacc ggggcggcgg cagggttagg cgagcatttt
    65401 acccgcttta aaacacgcta tctggcagga aaaacgcaac atctgggtag catcacagca
    65461 gaacagttag aaagcgttta aaatcattcg gtcacttctg cgggagaccg gtatgagtca
    65521 gcgaggactg gaagcactac tgcgaccaaa atcgatagcg gtaattggcg cgtcgatgaa
    65581 acccaatcgc gcaggttacc tgatgatgcg taacctgctg gcgggaggct ttaacggacc
    65641 ggtactcccg gtgacgccag cctggaaagc ggtgttgggt gtgttggcct ggccggatat
    65701 tgccagcttg ccctttacac ccgaccttgc ggttttatgt accaatgcca gccgtaatct
    65761 tgctcttctg gaagagctcg gcgagaaagg ctgtaaaacc tgcattattc tttccgcccc
    65821 ggcatcgcaa cacgaagatc tccgcgcctg cgccctgcgc cataacatgc gcctgcttgg
    65881 accaaacagt ctgggtttac tggctccctg gcaaggtctg aatgccagct tttcgcctgt
    65941 gccgattaaa cgcggcaagc tggcgtttat ttcacaatcg gctgccgtct ccaacaccat
    66001 cctcgactgg gcgcaacagc gtgagatggg cttttcctac tttattgcgc tcggcgacag
    66061 cctggatatc gacgttgatg aattgctcga ctatcttgcg cgcgacagta aaaccagcgc
    66121 catcctgctc tatctcgaac agttaagcga cgcgcgacgc tttgtttcgg cggcccgtag
    66181 tgcctcgcgt aataaaccga ttctggtgat taaaagcgga cgtagcccgg cggctcaacg
    66241 tctgttgaac accacggcag gaatggatcc ggcgtgggat gcggctattc agcgtgccgg
    66301 tttgttgcgg gtacaggaca cccacgagct gttttcggcg gtggaaaccc ttagccatat
    66361 gcgcccgctg cgtggcgacc ggttgatgat tatcagcaac ggtgctgcgc ctgccgcgct
    66421 ggcgctggat gccttatggt cacacaatgg caagctggca acgctaagcg aagaaacctg
    66481 ccagaaactg cgcgatgcac tgcctggaca tgtggcaatc tctaacccgc tcgatctgcg
    66541 cgatgacgcc agcagcgagc attatgtcaa aacgctggac attctgctcc acagccagga
    66601 ttttgatgcg ctgatggtta ttcattcacc cagcgcagcg gctcccgcaa cagaaagcgc
    66661 gcaagaatta attgaagcgg taaagcatca tccccgcagc aaatatgtct ctttgctgac
    66721 gaactggtgc ggcgagcact cctcgcaaga ggcacgacgt ttattcagcg aagccgggct
    66781 gccgacctac cgtaccccgg aaggaaccat cactgctttt atgcatatgg tggagtaccg
    66841 gcgtaatcag aagcaactac gcgaaacgcc ggcgttgccc agcaatctga cctccaatac
    66901 cgcagaagcg catcttctgt tgcaacaggc gattgccgaa ggagcaacgt cactcgatac
    66961 ccatgaagtt cagcctatct tgcaagcata tggcatgaac acgctcccca cctggattgc
    67021 cagcgatagc accgaagcgg tgcatattgc cgaacagatt ggttatccgg tggcgctgaa
    67081 attgcgttcg ccggatattc cacataaatc ggaagttcag ggcgtcatgc tctacctgcg
    67141 tacagccaat gaagtccagc aagcggcaaa cgccattttc gatcgcgtaa aaatggcctg
    67201 gccgcaggca cgggtccacg gcctgttggt gcaaagtatg gctaaccgtg ctggtgctca
    67261 ggagttgcgg gttgtggttg agcacgatcc ggttttcggg ccgttgatca tgctgggcga
    67321 aggcggtgtg gagtggcgtc ctgaagatca agccgtcgtc gcgttaccgc cgctgaacat
    67381 gaacctggcc cgctatctgg ttattcaggg gatcaaaagt aaaaagattc gtgcacgcag
    67441 tgcgctacgc ccattggatg ttgcaggctt gagccagctt ctggtgcagg tttccaactt
    67501 gattgtcgat tgcccggaaa ttcagcgtct ggatattcat cctttgctgg cttctggcag
    67561 tgaatttacc gcgctggatg tcacgctgga tatctcgccg tttgaaggcg ataacgagag
    67621 tcggctggca gtgcgccctt atccgcatca gctggaagaa tgggtagaat tgaaaaacgg
    67681 tgaacgctgc ttgttccgcc cgattttgcc agaagatgag ccacaacttc agcaattcat
    67741 ttcgcgagtc accaaagaag atctttatta ccgctacttt agcgagatca acgaatttac
    67801 ccatgaagat ttagccaaca tgacacagat cgactacgat cgggaaatgg cgtttgtagc
    67861 ggtacgacgt attgatcaaa cggaagagat cctcggcgtc acgcgtgcga tttccgatcc
    67921 tgataacatc gatgccgaat ttgctgtgct ggttcgctcg gatctcaaag ggttaggctt
    67981 aggtcgacgc ttaatggaaa agttgattac ctatacgcga gatcacggac tacaacgtct
    68041 gaatggtatt acgatgccaa acaatcgtgg catggtggcg ctagcccgca agctcgggtt
    68101 taacgttgat atccagctcg aagaggggat cgttgggctt acgctaaatc ttgcccaacg
    68161 cgaggaatca tgagtaaggt actggaaatg ttgaccactt aatcgggact ggtgttatta
    68221 ttgcccgctt atgtcgtctg cattgcacag aggacccttc aatgaacaga gaagaaatgc
    68281 actgtgatgt tgtcaaaatt taagcgtaat aaacatcaac aacaccttgc ccaactaccc
    68341 aagatttctc aatcagttga tgatgtcgat ttcttttacg ctcccgccga cttccgggag
    68401 acgctgctgg aaaaaatagc cagcgcgaag cagcgcattt gcattgtcgc cctgtatctc
    68461 gaacaggatg acggtggcaa aggcattctg aacgcgttgt atgaagctaa aaggcagcgt
    68521 ccggaactgg atgtgcgggt gctggtcgac tggcatcgtg cacaacgtgg acgcattggc
    68581 gctgcggcat ctaacactaa cgctgactgg tactgccgca tggcgcagga aaatccgggc
    68641 gtagatgttc cggtttatgg cgttccaatc aatacccgtg aagcccttgg tgttctgcac
    68701 tttaaaggct ttatcatcga cgatagcgta ctttatagcg gtgccagcct gaacgatgtt
    68761 tacctgcatc agcacgataa atatcgctac gaccgttatc atcttatccg taaccgtaag
    68821 atgtcagaca ttatgtttga atgggttaca cagaatatta tgaatggccg cggcgttaat
    68881 cgtctggatg atgttaatcg gccaaaaagc ccggaaatca agaacgatat tcgtctgttc
    68941 cgccaggagc tgcgtgatgc cgcttatcat ttccagggcg atgccgacaa cgatcagctt
    69001 tccgtaacgc cgctagtggg gctggggaaa tcgagtctgt tgaacaagac cattttccat
    69061 cttatgcctt gtgccgagca gaaactaacc atctgtacgc catacttcaa cctgccagca
    69121 atccttgtgc gcaatattat ccagttgctg cgcgaaggga aaaaggtcga aattattgtt
    69181 ggtgataaaa ccgcgaacga cttctacatt ccggaagatg aacctttcaa gataattggc
    69241 gcattgcctt atctctatga gatcaatctg cgtcgtttcc tgagccgttt gcagtattac
    69301 gtcaatactg accagctagt ggttcggtta tggaaagatg acgacaacac ctatcaccta
    69361 aaagggatgt gggttgatga taagtggatg ttgatcaccg gtaataacct gaacccgcgc
    69421 gcctggcgtc tggatctgga aaacgccatt ttgatccacg atccacaact ggagcttgcg
    69481 ccgcagcgag agaaagaact ggagctgatc cgcgagcata ccaccatcgt taagcactat
    69541 cgcgatctgc aaagtattgc cgattatccg gtgaaggttc gtaaactcat ccgccgtttg
    69601 cgccgtatcc gcatcgaccg attaattagc cgcatcctgt aatcacaacc ccgtcctgta
    69661 cggggtttgt tttttggagg ccacgttttg cgtattcttt ttgtctgttc gctattgtta
    69721 ctttctggat gcagccatat ggctaacgat agctggagcg ggcaggataa agctcaacac
    69781 tttatcgcct cggcgatgct ttccgccgcc ggaaatgaat attcacagca tcaggggatg
    69841 agccgggatc gcagtgccat gtttggattg atgttctctg tcagtttggg ggcgtcaaaa
    69901 gagctttggg atagccgccc cgaagggagc ggctggagct ggaaggattt ggcctgggat
    69961 gtcgccggtg caagcaccgg ctataccgtc tggcaactga cccgtcacta aagacgcatc
    70021 cccttccctt tgcgatgtag catcaaagaa accagaaacg ccaccacggc catcaaggtc
    70081 acataccaga agaaggctgt ttccattcct attgatttca gcgacaacgc tacgtactcc
    70141 gccgaaccac caaatatagc attagcgacc gcatatgaca ggccaacgcc taatgcgcga
    70201 acctgtgccg ggaacatctc agccttcagt attccactga ttgatgtata aaaactcact
    70261 atcagcaggg cacacatcac cagaccaaaa gcggcataag gcgaggaaac gttttgcaat
    70321 gctgagagaa taggaacggt aaaaatggct gccagcgaac cgaaacataa cattgaggta
    70381 cggcgaccaa tcttatccga cagcgcgcca atgagtggtt gaataagcat gaatacaaac
    70441 aatgcggcag tcataatgcc actcgccacg ttggcatgca ttcccgcagt atttaccaga
    70501 tacttctgca tataagtggt gaaggtatag aaacaaaggg agcccgcagc ggtaaaaccg
    70561 agaaccatga tgaatgcacg gcgattgcgc cataatcctt tcagagatcc agcttctttt
    70621 aaagcgcgcg tttcttgttg cgaagtttca tctaattgac gacgtaacca caacgccaca
    70681 acagctaaca cagctcctaa cgcgaaagga atacgccatc cccactctct gagtgcagcg
    70741 tcttccatgg tgtgttgtaa aaccacgaca accagtaggg ctagcagttg tccgccgatc
    70801 aacgtcacat actgaaatga tgcgtaaaaa cctttgcgc
//
LOCUS       DAZGAN020000013        65506 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGAN020000013 DAZGAN020000000
VERSION     DAZGAN020000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 65506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.013"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(25..504)
                     /gene="folK"
                     /locus_tag="ACHKYO_RS04880"
                     /old_locus_tag="pgaptmp_000976"
     CDS             complement(25..504)
                     /gene="folK"
                     /locus_tag="ACHKYO_RS04880"
                     /old_locus_tag="pgaptmp_000976"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGU4427866.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(501..1898)
                     /gene="pcnB"
                     /locus_tag="ACHKYO_RS04885"
                     /old_locus_tag="pgaptmp_000977"
     CDS             complement(501..1898)
                     /gene="pcnB"
                     /locus_tag="ACHKYO_RS04885"
                     /old_locus_tag="pgaptmp_000977"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HGU4427867.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1958..2854)
                     /gene="gluQRS"
                     /locus_tag="ACHKYO_RS04890"
                     /old_locus_tag="pgaptmp_000978"
     CDS             complement(1958..2854)
                     /gene="gluQRS"
                     /locus_tag="ACHKYO_RS04890"
                     /old_locus_tag="pgaptmp_000978"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HGU4427868.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEPGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2921..3376)
                     /gene="dksA"
                     /locus_tag="ACHKYO_RS04895"
                     /old_locus_tag="pgaptmp_000979"
     CDS             complement(2921..3376)
                     /gene="dksA"
                     /locus_tag="ACHKYO_RS04895"
                     /old_locus_tag="pgaptmp_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HGU4427869.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3554..4258)
                     /gene="sfsA"
                     /locus_tag="ACHKYO_RS04900"
                     /old_locus_tag="pgaptmp_000980"
     CDS             complement(3554..4258)
                     /gene="sfsA"
                     /locus_tag="ACHKYO_RS04900"
                     /old_locus_tag="pgaptmp_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HGU4427870.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAELSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4273..4803)
                     /gene="thpR"
                     /locus_tag="ACHKYO_RS04905"
                     /old_locus_tag="pgaptmp_000981"
     CDS             complement(4273..4803)
                     /gene="thpR"
                     /locus_tag="ACHKYO_RS04905"
                     /old_locus_tag="pgaptmp_000981"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HGU4427871.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4877..7306
                     /gene="hrpB"
                     /locus_tag="ACHKYO_RS04910"
                     /old_locus_tag="pgaptmp_000982"
     CDS             4877..7306
                     /gene="hrpB"
                     /locus_tag="ACHKYO_RS04910"
                     /old_locus_tag="pgaptmp_000982"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HGU4427872.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7502..10036
                     /gene="mrcB"
                     /locus_tag="ACHKYO_RS04915"
                     /old_locus_tag="pgaptmp_000983"
     CDS             7502..10036
                     /gene="mrcB"
                     /locus_tag="ACHKYO_RS04915"
                     /old_locus_tag="pgaptmp_000983"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HGU4427873.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10256..12499
                     /gene="fhuA"
                     /locus_tag="ACHKYO_RS04920"
                     /old_locus_tag="pgaptmp_000984"
     CDS             10256..12499
                     /gene="fhuA"
                     /locus_tag="ACHKYO_RS04920"
                     /old_locus_tag="pgaptmp_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HGU4427874.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNDGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12550..13347
                     /gene="fhuC"
                     /locus_tag="ACHKYO_RS04925"
                     /old_locus_tag="pgaptmp_000985"
     CDS             12550..13347
                     /gene="fhuC"
                     /locus_tag="ACHKYO_RS04925"
                     /old_locus_tag="pgaptmp_000985"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HGU4427875.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13347..14237
                     /gene="fhuD"
                     /locus_tag="ACHKYO_RS04930"
                     /old_locus_tag="pgaptmp_000986"
     CDS             13347..14237
                     /gene="fhuD"
                     /locus_tag="ACHKYO_RS04930"
                     /old_locus_tag="pgaptmp_000986"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HGU4427876.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPLLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14234..16216
                     /gene="fhuB"
                     /locus_tag="ACHKYO_RS04935"
                     /old_locus_tag="pgaptmp_000987"
     CDS             14234..16216
                     /gene="fhuB"
                     /locus_tag="ACHKYO_RS04935"
                     /old_locus_tag="pgaptmp_000987"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HGU4427877.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQATWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGID
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQIR"
     gene            complement(16251..17531)
                     /gene="hemL"
                     /locus_tag="ACHKYO_RS04940"
                     /old_locus_tag="pgaptmp_000988"
     CDS             complement(16251..17531)
                     /gene="hemL"
                     /locus_tag="ACHKYO_RS04940"
                     /old_locus_tag="pgaptmp_000988"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HGU4427878.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17697..17744
                     /gene="yadX"
                     /locus_tag="ACHKYO_RS04945"
                     /old_locus_tag="pgaptmp_000989"
     CDS             17697..17744
                     /gene="yadX"
                     /locus_tag="ACHKYO_RS04945"
                     /old_locus_tag="pgaptmp_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HGU4427879.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17756..19177
                     /gene="clcA"
                     /locus_tag="ACHKYO_RS04950"
                     /old_locus_tag="pgaptmp_000990"
     CDS             17756..19177
                     /gene="clcA"
                     /locus_tag="ACHKYO_RS04950"
                     /old_locus_tag="pgaptmp_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HGU4427880.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19201..19266
                     /gene="yadW"
                     /locus_tag="ACHKYO_RS04955"
                     /old_locus_tag="pgaptmp_000991"
     CDS             19201..19266
                     /gene="yadW"
                     /locus_tag="ACHKYO_RS04955"
                     /old_locus_tag="pgaptmp_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HGU4427881.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19259..19603
                     /gene="erpA"
                     /locus_tag="ACHKYO_RS04960"
                     /old_locus_tag="pgaptmp_000992"
     CDS             19259..19603
                     /gene="erpA"
                     /locus_tag="ACHKYO_RS04960"
                     /old_locus_tag="pgaptmp_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HGU4427882.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19650..20273)
                     /gene="yadS"
                     /locus_tag="ACHKYO_RS04965"
                     /old_locus_tag="pgaptmp_000993"
     CDS             complement(19650..20273)
                     /gene="yadS"
                     /locus_tag="ACHKYO_RS04965"
                     /old_locus_tag="pgaptmp_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HGU4427883.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20311..21111)
                     /gene="btuF"
                     /locus_tag="ACHKYO_RS04970"
                     /old_locus_tag="pgaptmp_000994"
     CDS             complement(20311..21111)
                     /gene="btuF"
                     /locus_tag="ACHKYO_RS04970"
                     /old_locus_tag="pgaptmp_000994"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HGU4427884.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLQDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21104..21802)
                     /gene="mtnN"
                     /locus_tag="ACHKYO_RS04975"
                     /old_locus_tag="pgaptmp_000995"
     CDS             complement(21104..21802)
                     /gene="mtnN"
                     /locus_tag="ACHKYO_RS04975"
                     /old_locus_tag="pgaptmp_000995"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGU4427885.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21886..23403
                     /gene="dgt"
                     /locus_tag="ACHKYO_RS04980"
                     /old_locus_tag="pgaptmp_000996"
     CDS             21886..23403
                     /gene="dgt"
                     /locus_tag="ACHKYO_RS04980"
                     /old_locus_tag="pgaptmp_000996"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HGU4427886.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23533..24957
                     /gene="degP"
                     /locus_tag="ACHKYO_RS04985"
                     /old_locus_tag="pgaptmp_000997"
     CDS             23533..24957
                     /gene="degP"
                     /locus_tag="ACHKYO_RS04985"
                     /old_locus_tag="pgaptmp_000997"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HGU4427887.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25112..26269
                     /gene="cdaR"
                     /locus_tag="ACHKYO_RS04990"
                     /old_locus_tag="pgaptmp_000998"
     CDS             25112..26269
                     /gene="cdaR"
                     /locus_tag="ACHKYO_RS04990"
                     /old_locus_tag="pgaptmp_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HGU4427888.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26358..26744)
                     /gene="yaeH"
                     /locus_tag="ACHKYO_RS04995"
                     /old_locus_tag="pgaptmp_000999"
     CDS             complement(26358..26744)
                     /gene="yaeH"
                     /locus_tag="ACHKYO_RS04995"
                     /old_locus_tag="pgaptmp_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HGU4427889.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27059..27883)
                     /gene="dapD"
                     /locus_tag="ACHKYO_RS05000"
                     /old_locus_tag="pgaptmp_001000"
     CDS             complement(27059..27883)
                     /gene="dapD"
                     /locus_tag="ACHKYO_RS05000"
                     /old_locus_tag="pgaptmp_001000"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HGU4427890.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27914..30586)
                     /gene="glnD"
                     /locus_tag="ACHKYO_RS05005"
                     /old_locus_tag="pgaptmp_001001"
     CDS             complement(27914..30586)
                     /gene="glnD"
                     /locus_tag="ACHKYO_RS05005"
                     /old_locus_tag="pgaptmp_001001"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HGU4427891.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30648..31442)
                     /gene="map"
                     /locus_tag="ACHKYO_RS05010"
                     /old_locus_tag="pgaptmp_001002"
     CDS             complement(30648..31442)
                     /gene="map"
                     /locus_tag="ACHKYO_RS05010"
                     /old_locus_tag="pgaptmp_001002"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HGU4427892.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31810..32535
                     /gene="rpsB"
                     /locus_tag="ACHKYO_RS05015"
                     /old_locus_tag="pgaptmp_001003"
     CDS             31810..32535
                     /gene="rpsB"
                     /locus_tag="ACHKYO_RS05015"
                     /old_locus_tag="pgaptmp_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGU4427893.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32793..33644
                     /gene="tsf"
                     /locus_tag="ACHKYO_RS05020"
                     /old_locus_tag="pgaptmp_001004"
     CDS             32793..33644
                     /gene="tsf"
                     /locus_tag="ACHKYO_RS05020"
                     /old_locus_tag="pgaptmp_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGU4427894.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33791..34516
                     /gene="pyrH"
                     /locus_tag="ACHKYO_RS05025"
                     /old_locus_tag="pgaptmp_001005"
     CDS             33791..34516
                     /gene="pyrH"
                     /locus_tag="ACHKYO_RS05025"
                     /old_locus_tag="pgaptmp_001005"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGU4427895.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34808..35365
                     /gene="frr"
                     /locus_tag="ACHKYO_RS05030"
                     /old_locus_tag="pgaptmp_001006"
     CDS             34808..35365
                     /gene="frr"
                     /locus_tag="ACHKYO_RS05030"
                     /old_locus_tag="pgaptmp_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGU4427896.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35457..36653
                     /gene="ispC"
                     /locus_tag="ACHKYO_RS05035"
                     /old_locus_tag="pgaptmp_001007"
     CDS             35457..36653
                     /gene="ispC"
                     /locus_tag="ACHKYO_RS05035"
                     /old_locus_tag="pgaptmp_001007"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HGU4427897.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36842..37600
                     /gene="ispU"
                     /locus_tag="ACHKYO_RS05040"
                     /old_locus_tag="pgaptmp_001008"
                     /gene_synonym="uppS"
     CDS             36842..37600
                     /gene="ispU"
                     /locus_tag="ACHKYO_RS05040"
                     /old_locus_tag="pgaptmp_001008"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HGU4427898.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37613..38470
                     /gene="cdsA"
                     /locus_tag="ACHKYO_RS05045"
                     /old_locus_tag="pgaptmp_001009"
     CDS             37613..38470
                     /gene="cdsA"
                     /locus_tag="ACHKYO_RS05045"
                     /old_locus_tag="pgaptmp_001009"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4427899.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38482..39834
                     /gene="rseP"
                     /locus_tag="ACHKYO_RS05050"
                     /old_locus_tag="pgaptmp_001010"
     CDS             38482..39834
                     /gene="rseP"
                     /locus_tag="ACHKYO_RS05050"
                     /old_locus_tag="pgaptmp_001010"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HGU4427900.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39864..42296
                     /gene="bamA"
                     /locus_tag="ACHKYO_RS05055"
                     /old_locus_tag="pgaptmp_001011"
     CDS             39864..42296
                     /gene="bamA"
                     /locus_tag="ACHKYO_RS05055"
                     /old_locus_tag="pgaptmp_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HGU4427901.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42418..42903
                     /gene="skp"
                     /locus_tag="ACHKYO_RS05060"
                     /old_locus_tag="pgaptmp_001012"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42418..42903
                     /gene="skp"
                     /locus_tag="ACHKYO_RS05060"
                     /old_locus_tag="pgaptmp_001012"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HGU4427902.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42907..43932
                     /gene="lpxD"
                     /locus_tag="ACHKYO_RS05065"
                     /old_locus_tag="pgaptmp_001013"
     CDS             42907..43932
                     /gene="lpxD"
                     /locus_tag="ACHKYO_RS05065"
                     /old_locus_tag="pgaptmp_001013"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HGU4427903.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44037..44492
                     /gene="fabZ"
                     /locus_tag="ACHKYO_RS05070"
                     /old_locus_tag="pgaptmp_001014"
     CDS             44037..44492
                     /gene="fabZ"
                     /locus_tag="ACHKYO_RS05070"
                     /old_locus_tag="pgaptmp_001014"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGU4427904.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44496..45284
                     /gene="lpxA"
                     /locus_tag="ACHKYO_RS05075"
                     /old_locus_tag="pgaptmp_001015"
     CDS             44496..45284
                     /gene="lpxA"
                     /locus_tag="ACHKYO_RS05075"
                     /old_locus_tag="pgaptmp_001015"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HGU4427905.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45284..46432
                     /gene="lpxB"
                     /locus_tag="ACHKYO_RS05080"
                     /old_locus_tag="pgaptmp_001016"
     CDS             45284..46432
                     /gene="lpxB"
                     /locus_tag="ACHKYO_RS05080"
                     /old_locus_tag="pgaptmp_001016"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HGU4427906.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46429..47025
                     /gene="rnhB"
                     /locus_tag="ACHKYO_RS05085"
                     /old_locus_tag="pgaptmp_001017"
     CDS             46429..47025
                     /gene="rnhB"
                     /locus_tag="ACHKYO_RS05085"
                     /old_locus_tag="pgaptmp_001017"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGU4427907.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47062..50544
                     /gene="dnaE"
                     /locus_tag="ACHKYO_RS05090"
                     /old_locus_tag="pgaptmp_001018"
     CDS             47062..50544
                     /gene="dnaE"
                     /locus_tag="ACHKYO_RS05090"
                     /old_locus_tag="pgaptmp_001018"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGU4427908.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50557..51516
                     /gene="accA"
                     /locus_tag="ACHKYO_RS05095"
                     /old_locus_tag="pgaptmp_001019"
     CDS             50557..51516
                     /gene="accA"
                     /locus_tag="ACHKYO_RS05095"
                     /old_locus_tag="pgaptmp_001019"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGU4427909.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51615..53756
                     /gene="ldcC"
                     /locus_tag="ACHKYO_RS05100"
                     /old_locus_tag="pgaptmp_001020"
     CDS             51615..53756
                     /gene="ldcC"
                     /locus_tag="ACHKYO_RS05100"
                     /old_locus_tag="pgaptmp_001020"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HGU4427910.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53813..54202
                     /gene="yaeR"
                     /locus_tag="ACHKYO_RS05105"
                     /old_locus_tag="pgaptmp_001021"
     CDS             53813..54202
                     /gene="yaeR"
                     /locus_tag="ACHKYO_RS05105"
                     /old_locus_tag="pgaptmp_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4427911.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54267..55565
                     /gene="tilS"
                     /locus_tag="ACHKYO_RS05110"
                     /old_locus_tag="pgaptmp_001022"
     CDS             54267..55565
                     /gene="tilS"
                     /locus_tag="ACHKYO_RS05110"
                     /old_locus_tag="pgaptmp_001022"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGU4427912.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWINSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55614..55874)
                     /gene="rof"
                     /locus_tag="ACHKYO_RS05115"
                     /old_locus_tag="pgaptmp_001023"
     CDS             complement(55614..55874)
                     /gene="rof"
                     /locus_tag="ACHKYO_RS05115"
                     /old_locus_tag="pgaptmp_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HGU4427913.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55861..56061)
                     /gene="yaeP"
                     /locus_tag="ACHKYO_RS05120"
                     /old_locus_tag="pgaptmp_001024"
     CDS             complement(55861..56061)
                     /gene="yaeP"
                     /locus_tag="ACHKYO_RS05120"
                     /old_locus_tag="pgaptmp_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HGU4427914.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56227..56772
                     /gene="yaeQ"
                     /locus_tag="ACHKYO_RS05125"
                     /old_locus_tag="pgaptmp_001025"
     CDS             56227..56772
                     /gene="yaeQ"
                     /locus_tag="ACHKYO_RS05125"
                     /old_locus_tag="pgaptmp_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HGU4427915.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     VLFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56769..57191
                     /gene="arfB"
                     /locus_tag="ACHKYO_RS05130"
                     /old_locus_tag="pgaptmp_001026"
     CDS             56769..57191
                     /gene="arfB"
                     /locus_tag="ACHKYO_RS05130"
                     /old_locus_tag="pgaptmp_001026"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HGU4427916.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57205..57915
                     /gene="nlpE"
                     /locus_tag="ACHKYO_RS05135"
                     /old_locus_tag="pgaptmp_001027"
                     /gene_synonym="cutF"
     CDS             57205..57915
                     /gene="nlpE"
                     /locus_tag="ACHKYO_RS05135"
                     /old_locus_tag="pgaptmp_001027"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HGU4427917.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDALSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPMLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(58070..58893)
                     /gene="yaeF"
                     /locus_tag="ACHKYO_RS05140"
                     /old_locus_tag="pgaptmp_001028"
                     /pseudo
     CDS             complement(58070..58893)
                     /gene="yaeF"
                     /locus_tag="ACHKYO_RS05140"
                     /old_locus_tag="pgaptmp_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(58947..60665)
                     /gene="proS"
                     /locus_tag="ACHKYO_RS05145"
                     /old_locus_tag="pgaptmp_001029"
     CDS             complement(58947..60665)
                     /gene="proS"
                     /locus_tag="ACHKYO_RS05145"
                     /old_locus_tag="pgaptmp_001029"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGU4427918.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60777..61484)
                     /gene="tsaA"
                     /locus_tag="ACHKYO_RS05150"
                     /old_locus_tag="pgaptmp_001030"
     CDS             complement(60777..61484)
                     /gene="tsaA"
                     /locus_tag="ACHKYO_RS05150"
                     /old_locus_tag="pgaptmp_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HGU4427919.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDVGFEVFALEPR"
     gene            complement(61481..61885)
                     /gene="rcsF"
                     /locus_tag="ACHKYO_RS05155"
                     /old_locus_tag="pgaptmp_001031"
     CDS             complement(61481..61885)
                     /gene="rcsF"
                     /locus_tag="ACHKYO_RS05155"
                     /old_locus_tag="pgaptmp_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HGU4427920.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62003..62818)
                     /gene="metQ"
                     /locus_tag="ACHKYO_RS05160"
                     /old_locus_tag="pgaptmp_001032"
     CDS             complement(62003..62818)
                     /gene="metQ"
                     /locus_tag="ACHKYO_RS05160"
                     /old_locus_tag="pgaptmp_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HGU4427921.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62858..63511)
                     /gene="metI"
                     /locus_tag="ACHKYO_RS05165"
                     /old_locus_tag="pgaptmp_001033"
     CDS             complement(62858..63511)
                     /gene="metI"
                     /locus_tag="ACHKYO_RS05165"
                     /old_locus_tag="pgaptmp_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HGU4427922.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63504..64535)
                     /gene="metN"
                     /locus_tag="ACHKYO_RS05170"
                     /old_locus_tag="pgaptmp_001034"
     CDS             complement(63504..64535)
                     /gene="metN"
                     /locus_tag="ACHKYO_RS05170"
                     /old_locus_tag="pgaptmp_001034"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HGU4427923.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64723..65298
                     /gene="gmhB"
                     /locus_tag="ACHKYO_RS05175"
                     /old_locus_tag="pgaptmp_001035"
     CDS             64723..65298
                     /gene="gmhB"
                     /locus_tag="ACHKYO_RS05175"
                     /old_locus_tag="pgaptmp_001035"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HGU4427924.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15445 a        16332 c        18068 g        15661 t
ORIGIN      
        1 atttatattc gtaatataaa taaattacca tttgcttaat ttgtcaaatg ctcttgtatg
       61 taagacttca cgcaacgtct ccccatcagg aaacgccagc tccggtgcaa tctcaaacag
      121 cggccacagc ataaatccac gattcttcat atcgtagtgc ggaacggtca ggcgttcggt
      181 atttatcact tcattaccaa acagcatgat atcgagatcc agcgtgcgtg gtccccagcg
      241 ttcagcttta cgaacacgac cctgttgcag ttctatgcgc tgtgtatggt tgagtaactc
      301 ttccggggcg agcgaagttt caagcgccac ggcggcgttg aggtaatcgg gttgatcttg
      361 cggccccagc ggtggggtgc ggtaaaacga agaaacggca agaatgtggc tttcagggat
      421 atcgcctaat gctttcaggg cagcattgac ctgctccagc ggagaggcca gattgctgcc
      481 tatggcaata tacgccactg tcatgcggta ccctcacgac gcggtgcgcg tttgcgtgga
      541 cgacgagtac ggcgacgcgg tgacggctct tcatccaact cgttaagcat ccctttttga
      601 tctggtggcg cggaaacctg gaactcaccc caccatttca ccagacgctg cagttcagcg
      661 ttacgttcaa cttcagctcg caaggccaac aggtcataag ccgcacggaa cttaggatgc
      721 tccagcagtt tccatgcgcg tttaccctga cgacgggaca tacgcaactg caactgccag
      781 atatcgcggg ttaacgtcgt cagacgtttc gggattgcca gtgaacggca ggcttcgtcc
      841 agcacgtcgt tcatcgccag cgcgaaagcg tcgtgatagg tcaggccgct ttcctgggca
      901 atcttctgtg ccgtctccag cagtgggtac cagaacatgg cggcaaacag gaacgccggg
      961 ttcacgcgca tatcgttatg gatacgcgta tcggtattct tcagcacctg ttcaatgatc
     1021 cgctccatcg ggctgtcgcc attttccgtg aagtagcggg taatggtcgg gaacagcggc
     1081 tggaacagat gatattcaca caacagctta taggtatcgt aaccgtagcc cgcttgtagc
     1141 agtttaagcg attcttcaaa caggcgtgcc ggtgggatat cgttcaacag ggtagcgagg
     1201 cgagggatcg gttctgcggt ttccgggctg atgcgcatac ccaatttggc ggcaaaacgt
     1261 accgcgcgca gcatacgtac cggatcttca cggtagcgcg tttccgggtt accaatcaga
     1321 cggataacgc cgtccttcag atccttcata ccgccaacgt aatcacggac ggtaaaatcc
     1381 gctacgctgt aatagaggct gttgatagtg aaatcgcggc gctgggcgtc ttcttcgatg
     1441 gagccgaaaa tgttgtcgcg cagcaacatg ccgttttgcc cgcgttggga ggtcgtgcgg
     1501 tcgctgacgt taccttcgtg gtgtccacgg aaggtcgcaa cttcgataat ctccgggcca
     1561 aacattacat gagccagacg gaaacggcga cccaccaggc ggcagttacg gaacagtttg
     1621 cgcacctgct caggcgtggc gttagtggtt acgtcaaaat ctttcggctt tttgccaagt
     1681 aacaggtcgc gcacgccgcc gccaaccagc caggcttcgt atcccgcttt gttgagcctg
     1741 tacattacct tcagggcatt ttcactgata tctttgcggg aaatagcatg ctgctcacgc
     1801 gggatcaccg tcacctgtgg acgggcgact gcctgttcag cctcgctttc ctcgcggctt
     1861 agcaccttgc ggcaaaaatt agcgactcgg gtaaaaatag tacacctcgg tagtgtcaaa
     1921 catcattcag gacaaaaaaa tagcggctaa tcatagctca gcatgacgca tttgagaatg
     1981 ttgaatttac aattgccgac tcgggcacgg cggtaagccg ccagtttttg acggctgact
     2041 gaaggatttg ctcgacgctg aaatcctgcc agtgtgcttc tgcctgctgc cccagaaatt
     2101 gaagtgccgc gattagtacc gggcgtggat cgcctttcgg caacgcaggc gcatgattct
     2161 gcttggaaag tttagcgcct tgtggattaa gcgccagcgg cagatgaatg taatctggca
     2221 ctttccagcc aaaaagctgg tacagcgaga tttgccttac tgttggttca atcagatcag
     2281 ccccacgcac tatttctgta acgccctgga aatgatcatc aaccacaaca gccaggttgt
     2341 aggcgaacaa cccatcacgg cgatgaatga taaaatcttc ccgtgccagt ttttcgtcgg
     2401 cgtgaataat gccgcgcagc tggtcagtaa attgcgtgac cggatgctgc tggcggatac
     2461 gcactgcggc attgtctggt ccatgatgca acacccggca atgaccgtcg taaataccgc
     2521 caatgctttg aatacgcgca cgcgtacagg tgcagtaata acttagtcct ggttcatgta
     2581 accaggcgag tgcttcacga taggcgtggt gacgttgcga ttgccagaga acatcgccgt
     2641 cccagtgcag accgtaatgt tccagctggc gcaggatagt ttctgcggca ccgggaactt
     2701 cacgaggcgg gtcgatatct tctatgcgta ccagccagcg accttgccgg gcgcgagcct
     2761 gcaaatagct gccgagcgcg gcgatcagag agccaaaatg aagctcgccg gaaggagagg
     2821 gggcgaagcg gccaatatac tgtgtgtctg tcatctcttt gaacaaaaaa taaggcggga
     2881 gcatttcccg cctgtggtaa acgtgatgga acggctgtaa ttagccagcc atctgttttt
     2941 cgcgaatttc agccagcgtt ttgcagtcga tgcacagatc ggctgtcggg cgcgcttcca
     3001 gacggcgaat accaatttca acaccgcagg attcgcagta gccgaaatct tcgtcttcca
     3061 cttttttcag cgtcttctcg atctttttga tcagcttacg ctcgcgatcg cggttacgca
     3121 gttcgaggct gaactcttct tcctgggctg cacggtctac cgggtccggg aagttggctg
     3181 cttcatcctg catatgtgta acggtgcgat cgacttcatc cctgagttga ttacgccatg
     3241 cttccagaat acgacggaag tgcgccagct gggcttcatt catatactct tcgcccggct
     3301 tctcctgata tggttccacc ccagcgatgg cgagaatact cagggacgat gttttacggt
     3361 tttgcccttc ttgcatgttg cttctcctta acacgcacta tcgatcccca tgttcggggg
     3421 aaaaatgagg ccgctataaa tagcagatgc ttttccggat agcaattatc taaacgtaac
     3481 acttgacaac tgtgtgagga aaagcgtatt tgcgcacgcg accagaatgt aaattaacca
     3541 gttacttact ttactacaat gtaaccggca gtgatttttt aagagccatg ccttcagcag
     3601 aaatttccgc tttgtaagcc agaatttcta cccccctctg ttgagcttct gacaatagtt
     3661 gcgcgtattt ctcatcgatg tggcgcgcgg gtgaaaaccg tgtaatggct gaatgcagca
     3721 cggcgaaaaa gataaccgca cgctggcctt cagccgctac gctcatcaac tcccgaaggt
     3781 gtttctgacc tcgttcagtg accgcatcgg gaaaatatcc ctgttcgttc tccgctaacg
     3841 taaccgattt cacttcaata tagcagtctg gacgcgaatc cgcctgcaac ataaagtcaa
     3901 tacggctgag ttctgcgccg tattttactt cgcttttcag cgagctatag cctgacagtt
     3961 ctgaaattga ttcattaagg atagcctctt tcgtcaacct gttagcccaa agcgtgttga
     4021 cgcaaataaa tgcgccgctc tggctttgag ttaattccca ggtgtgtggg tatttccgtt
     4081 tggtgttgtc tgaagtcgaa taccagacgg tatcgccagg cgttgcacaa ccggtcattg
     4141 cacccgtatt cgggcagtgt agcgttaatt cgcgaccatc gggtgtgatc acatcggcta
     4201 aaaaacgttt gtaacgctga attagcgtcg cgcgctgtag agggggagag aattccattg
     4261 cgacaatcct tgttattgcg ttagcgccca gcgttttagc ggcgtgtagc gtgtgcgtcc
     4321 acgggcaaac gaggaggcgt aaagggtgaa ctccgtcacc gcataagacc agttaaaacc
     4381 tggcggcggg attgtcaccg cctcgctggc gtcgcgcaat aaggtaatat gtggatgaaa
     4441 cggacgattg ctttgaaaac aaccgctgcg ggcagcctgt gaacggagca tattcgccag
     4501 ctggattaag ccgcgtggag gctgacgcat ccctaaccac accacacgcg aacgcagcca
     4561 ttgtccggcg tcatcaagcg tgagtgtgaa accaggttga cgaatccgtc cggctaaaag
     4621 agaaagcgcc ttctctttct cggcgctcac ttcgcctaaa aatgccagag tcagatgcaa
     4681 attatcggcg gcgaccggac gtcccgcctc aggtgggaag tgtgtggcgc gccaatggat
     4741 aatctgttcg cggatttctg caggtaagtc gatagcaaag aacagacgtt gcggttcaga
     4801 catgtgaggc actcggttat gaattaccgc gatgctacaa tgtggcgcga agaatgttaa
     4861 ccctctggag cgttttgtgt cgtcgttgcc cgttgctgcc gtcttatctg aattacttac
     4921 cgctctcgat tgtgcgccgc aggtattatt aagtgcgccg accggggccg ggaaatcaac
     4981 ctggctgccg ctgcaactgc tggcgcatcc cggcattaac gggaaaatta tcctgctgga
     5041 gccgcgtcgt ctggcggcgc gtaacgtcgc gcaacggctg gcggagctgc ttaacgaaaa
     5101 gccaggcgat accgttggct accggatgcg tgcgcaaaac tgcgtcgggc cgaatacccg
     5161 cctggaagtg gttaccgaag gcgtgctgac gcgcatgatc cagcgtgacc cggaactgag
     5221 cggtgttgga ctggtgatcc ttgatgaatt tcatgagcgc agcttgcagg cggatttggc
     5281 gttggcgctg ttactcgatg tgcaacaagg tctgcgtgat gaccttaaac tgctgattat
     5341 gtcggctacc ctggacaacg accgcttgca gcaaatgctg ccagaagcgc ctgtcgtcat
     5401 ctcagaaggg cgctcgtttc cggttgaacg ccgttattta ccgctgcccg cgcatcagcg
     5461 ttttgacgat gccgttgcgg tagccaccgc tgaaatgctg cgtcaggaaa gcggatcatt
     5521 actgttattt ttacctggcg tcggagaaat tcagcgtgtg caggaacaac tggcttcgcg
     5581 catcggcagt gatgtattgc tctgcccgct gtatggcgcg ttgtcgctga acgatcagcg
     5641 aaaagcgatc ctcccggcac cgcaagggat gcgcaaagtg gtgctggcga ccaatattgc
     5701 tgaaaccagt ttaaccattg aaggtattcg tctggtggtg gattgtgccc aggagcgtgt
     5761 ggcgcgtttt gatccgcgca cggggcttac gcgactgatt actcaacgcg ttagccaggc
     5821 atccatgacg cagcgtgccg ggcgcgccgg gcgtctggag ccgggtatct gcctgcattt
     5881 aatcgccaaa gaacaagcag aacgcgccgc ggcgcaaagt gaaccggaga tcttacaaag
     5941 cgatctttcc ggtttgctga tggaattact gcaatgggga tgcagcgatc cggcgcagat
     6001 gagctggctg gatcaaccgc cagtagtgaa tctactggcc gcgaaacgtc tgttacaaat
     6061 gctgggggca ctggagggtg aacggcttag tgcgcaaggg caaaaaatgg cagcgctggg
     6121 taacgatccg cgtttagcgg caatgctggt tagcgcgaag aacgacgacg aagctgctac
     6181 cgcggcaaaa attgccgcca ttctcgaaga gccgccacgg atgggcaata gtgacctggg
     6241 cgtggcgttt tcgcgcaatc aaccagcctg gcagcaacgt agtcagcaac tgttaaaacg
     6301 cttaaacgta cgtggcggtg aggcagacag ttcgcttatc gcgccgctac ttgccggggc
     6361 gtttgccgat cgcattgctc gtcgccgtgg gcaagatgga cgctatcaac tggcaaacgg
     6421 catgggagcg atgctcgatg ccaacgacgc gctaagccgc cacgaatggt tgatcgcacc
     6481 gttattattg cagggcagcg cctcgccgga tgcgcggatt ttactggcgc tgccggtcga
     6541 tattgatgag ttagtacaac gctgcccgca gctggtacag cagtctgaca ctgtggagtg
     6601 ggatgacgcg caaggtacgc tgaaagcctg gcgtcggcta caaatcggtc agttgacggt
     6661 gaaagtgcag ccgctggcga aaccgtcaga agacgagttg catcaggcga tgcttaatgg
     6721 catccgtgat aaaggtttaa gcgtgctcaa ctggacggcg gaagcggaac agctacgctt
     6781 gcgtttgtta tgcgccgcaa agtggttgcc ggaatatgac tggccagcgg ttgatgatga
     6841 aagtttattg gcagcgctgg aaacgtggct gctgccacat atgactggcg tacattcact
     6901 acgcggcctg aaatcactcg acatttatca ggcactacgc ggattacttg attggggaat
     6961 gcagcaacgt ctggatagtg aattgcctgc gcattacact gtgccgacgg gaagccggat
     7021 cgccattcgt tatcatgaag ataacccgcc cgcgctggcg gtgagaatgc aagagatgtt
     7081 tggcgaggcc accaatccga cgatcgccca ggggcgcgtg ccgctggtgc tggagttgct
     7141 ttcacctgcc caaaggccat tacaaatcac acgagatttg agcgccttct ggaaaggagc
     7201 gtaccgtgag gtgcaaaaag agatgaaagg gcgttatccc aaacatgtct ggccggacga
     7261 cccggcaaat actgcaccga cgcgacggac gaaaaagtat tcgtaaggtt ggttttctcc
     7321 ctctccctgt gggagagggc cggggtgagg gcatcagcgc gcacgttcac cctcacccta
     7381 accctctccc tcaagggaga ggggaccgat cgagcacaaa ttttgagaga tatcttcttc
     7441 tgtcttgtaa cagaagaaca gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag
     7501 catggccggg aatgaccgcg agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa
     7561 acaaaaggta agccgtcgtc gttacgaaga tgacgatgat tacgacgatt atgatgacta
     7621 tgaggatgaa gaaccgatgc cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg
     7681 tggcaaacgc ggctggctat ggctactgct aaaactggct atcgtttttg ccgtgctgat
     7741 cgccatttac ggcgtttatc tcgatcaaaa aattcgtagc cgtattgatg gcaaggtctg
     7801 gcaactgcct gcggcagttt atggccgaat ggtcaatctt gagccagaca tgaccatcag
     7861 caagaacgag atggtgaagc tgctggaggc gacccagtat cgtcaggtgt cgaaaatgac
     7921 ccgtcctggc gaatttaccg tgcaggccaa cagcattgag atgattcgcc gtccgtttga
     7981 tttcccggac agtaaagaag gacaggtgcg cgcgcgtctg acctttgatg gcgatcatct
     8041 ggcgacgatc gtcaatatgg agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg
     8101 tctgatcacc atgatctctt cgccaaacgg tgagcagcgt ctgtttgtgc cgcgcagtgg
     8161 tttcccggat ttgctggtgg atactttgct ggcgacagaa gaccgtcatt tttacgagca
     8221 tgatggaatc agtctctact caatcggacg tgcggtgctg gcaaacctga ccgccggacg
     8281 cacggtacag ggtgcgagta cgctgacgca acagctggtg aaaaacctgt tcctctccag
     8341 cgagcgttct tactggcgta aagcgaacga agcttacatg gcgctgatca tggacgcgcg
     8401 ttacagcaaa gaccgtattc ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg
     8461 cgacaacgaa atccgcggct tcccgctggc aagcttgtat tactttggtc gcccggtaga
     8521 agagctaagc ctcgaccagc aggcgctgtt agtcggtatg gtgaaagggg cgtccatcta
     8581 caacccgtgg cgtaacccaa aactggcgct ggagcgacgt aatctggtgc tgcgtctgct
     8641 gcaacagcaa cagattattg atcaagaact ctatgacatg ttgagtgccc gtccgctggg
     8701 ggttcagccg cgcggtgggg tgatctctcc tcagccagcc tttatgcaac tggtgcgtca
     8761 ggagctgcag gcaaaactgg gcgataaggt aaaagatctc tccggcgtga agatcttcac
     8821 tacctttgac tcggtggccc aggacgcggc agaaaaagcc gccgtggaag gcattccggc
     8881 actgaagaaa cagcgtaagt tgagcgatct tgaaactgcg attgtggtcg tcgaccgctt
     8941 tagtggtgaa gttcgtgcga tggtcggagg ttctgagccg cagtttgcgg gctacaaccg
     9001 tgcgatgcag gcgcgtcgtt cgattggttc ccttgcaaaa ccagcgactt atctgacggc
     9061 tttaagccag ccgaaaatct atcgcctgaa cacgtggatt gccgatgcgc caatcgcgct
     9121 gcgtcagccg aatggccagg tctggtcacc gcagaacgat gaccgtcgtt atagcgaaag
     9181 cggcagagtg atgctggtgg atgcgttgac ccgttcgatg aacgtgccga cggtaaatct
     9241 ggggatggcg ctggggctgc ctgcagttac ggatacctgg atcaaactgg gcgtaccgaa
     9301 agatcagctg catccggttc cggcgatgct gctgggggcg ttgaacttaa cgccaatcga
     9361 agtggcgcag gcgttccaga ccatcgccag cggtggtaac cgtgcaccgc tttctgcact
     9421 gcgttcggta atcgcggaag atggcaaggt gctgtatcag agcttcccgc aggcagaacg
     9481 cgctgttccg gcgcaggcgg cgtatctgac actatggacc atgcagcagg tggtacaacg
     9541 tggtacgggt cgtcagcttg gggcgaaata cccgaacctg catctggcag ggaaaacagg
     9601 gactaccaac aataacgtag atacctggtt tgcaggtatt gacggcagca cggtgaccat
     9661 cacctgggta ggccgtgata acaaccagcc gaccaaactg tatggagcca gcggggcaat
     9721 gtcgatttat cagcgttatc tggctaacca gacgccaacg ccgctgaatc ttgttccgcc
     9781 agaagatatt gcagatatgg gcgtggacta cgacggcaac tttgtttgca gcggtggcat
     9841 gcgtgtcttg ccggtctgga ccagcgatcc gcaatcgctg tgccagcaga gcgagatgca
     9901 gcagcagccg tcaggcaatc cgtttgatca gtcttctcag ccgcagcaac agccgcaaca
     9961 gcaacctgct cagcaagagc agaaagacag cgacggtgta gccggttgga tcaaggatat
    10021 gtttggtagt aattaacatc taagcgtgaa ataccggatg gcgagttgcc atccggtaaa
    10081 ataacatccc atctaagata ttaacccttt cttttcatct ggttgtttat taacccttca
    10141 ggaacgctca gattgcgtac cgcttgcgaa cccgccagcg tttcgaatat tatcttatct
    10201 ttataataat cattctcgtt tacgttatca ttcactttac atcagagata taccaatggc
    10261 gcgttccaaa actgctcagc caaaacactc actgcgtaaa atcgcagttg tagtagccac
    10321 agcggtaagc ggcatgtctg tttatgcaca ggcagcggtt gaaccgaaag aagacactat
    10381 caccgttacc gctgcacctg cgccgcaaga aagcgcatgg gggcctgctg caactattgc
    10441 ggcgcgacag tctgctaccg gcactaaaac cgatacgccg attcaaaaag tgccacagtc
    10501 tatttctgtt gtgaccgccg aagagatggc gctgcatcag ccgaagtcgg taaaagaagc
    10561 gcttagctac acgccgggtg tctctgttgg tacgcgtggc gcatccaaca cctatgacca
    10621 cctgatcatt cgcggctttg cggcagaagg ccaaagccag aataactatc tgaatggcct
    10681 gaagttgcag ggcaacttct ataacgatgc ggtcattgac ccgtatatgc tggaacgcgc
    10741 tgaaattatg cgtggcccgg tttccgtgct ttacggtaaa agcagtcctg gcggcctgtt
    10801 gaatatggtc agcaagcgtc cgaccaccga accgctgaaa gaagttcagt ttaaagccgg
    10861 tactgacagc ctgttccaga ctggttttga ctttagcgat tcgttggatg atgacggtgt
    10921 ttactcttat cgcctgaccg gtcttgcgcg ttctgccaat gcccagcaga aagggtcaga
    10981 agagcagcgt tatgctattg caccggcgtt cacctggcgt ccggatgata aaaccaattt
    11041 taccttcctt tcttacttcc agaacgagcc ggaaaccggt tattacggct ggttgccgaa
    11101 agagggaacc gttgagccgc tgccgaacgg taagcgtctg ccgacagact ttaatgacgg
    11161 ggcgaagaac aacacctatt ctcgtaatga gaagatggtc ggctacagct tcgatcacga
    11221 atttaacgac acctttactg tgcgtcagaa cctgcgcttt gctgaaaaca aaacctcgca
    11281 aaacagcgtt tatggttacg gcgtctgctc cgatccggcg aatgcttaca gcaaacagtg
    11341 tgcggcatta gcgccagcgg ataaaggcca ttatctggca cgtaaatacg tcgttgatga
    11401 tgagaagctg caaaacttct ccgttgatac ccagttgcag agcaagtttg ccactggcga
    11461 tatcgaccac accctgctga ccggtgtcga ctttatgcgt atgcgtaatg acatcaacgc
    11521 ctggtttggt tacgacgact ctgtgccact gctcaatctg tacaatccgg tgaataccga
    11581 tttcgacttc aatgccaaag atccggcaaa ctccggccct taccgcattc tgaataaaca
    11641 gaaacaaacg ggcgtttatg ttcaggatca ggcgcagtgg gataaagtgc tggtcaccct
    11701 gggcggtcgt tatgactggg cagatcaaga atctcttaac cgcgttgctg ggacgaccga
    11761 taaacgtgat gacaaacagt ttacctggcg tggtggtgtt aactacctgt ttgataatgg
    11821 tgtaacacct tacttcagct atagcgaatc gtttgaacct tcttcgcaag ttgggaagga
    11881 tggtaatatt ttcgcaccgt ctaaaggtaa gcagtatgaa gtcggcgtga aatatgtacc
    11941 ggaagatcgt ccgattgtag ttactggtgc cgtgtataat ctcactaaaa ccaacaacct
    12001 gatggcggac cctgagggtt ccttcttctc ggttgaaggt ggcgagatcc gcgcacgtgg
    12061 cgtagaaatc gaagcgaaag cggcgctgtc ggcgagtgtt aacgtagtcg gttcttatac
    12121 ttacaccgat gcggaataca ccaccgatac tacctataaa ggcaatacgc ctgcacaggt
    12181 gccaaaacac atggcttcgc tgtgggctga ctataccttc tttgacggtc cgctttcagg
    12241 tctgacgctg ggcaccggtg gtcgttatac tggctccagc tatggtgatc cggctaactc
    12301 ctttaaagtg ggaagttata cggtcgtgga tgcgttagtg cgttatgatc tggcgcgagt
    12361 cggcatggcg ggctccaacg tggcgctgca tgttaacaac ctgttcgatc gtgaatacgt
    12421 cgccagctgc tttaacactt atggctgctt ctggggcgca gaacgtcagg tcgttgcaac
    12481 cgcaaccttc cgtttctaat ttctcttttg gggcacggat ttccgtgccc atttcacaag
    12541 ttggctgtta tgcaggaata cacgaatcat tccgatacca cttttgcact gcgtaatatc
    12601 tcctttcgtg tgcccgggcg cacgcttttg catccgctgt cgttaacctt tcctgccggg
    12661 aaagtgaccg gtctgattgg tcacaacggt tctggtaaat ccactctgct caaaatgctt
    12721 ggccgtcatc agcctccgtc agaaggggag attcttcttg atgcccagcc gctggaaagc
    12781 tggagcagca aagcgtttgc ccgcaaagtg gcttatttgc cgcagcagct tcctccggca
    12841 gaagggatga ccgtgcgtga actggtggcg attggtcgtt acccgtggca tggcgcgctg
    12901 gggcgctttg gtgcggcaga tcgcgaaaag gtcgaggaag ctatctcgct ggttggctta
    12961 aaaccgctgg cgcatcggct ggtcgatagt ctctctggcg gcgaacgtca gcgggcgtgg
    13021 atcgccatgc tggtggcgca ggatagccgt tgtctgttgc tcgacgaacc gacttcggcg
    13081 ctggatatcg cccaccaggt tgatgtgctg gcgctggtgc accgtttaag tcaggaacgt
    13141 ggcctgacgg tcattgccgt gctgcacgat atcaatatgg cggcacgcta ctgtgattat
    13201 ctggtcgccc tgcgcggtgg tgaaatgatt gctcagggaa cgcctgcgga aattatgcgc
    13261 ggcgaaaccc tcgaaatgat ttatggcatc ccgatgggta ttttgccgca tccggcgggt
    13321 gctgcacctg tgagttttgt ttattgatga gcggcttacc tcttatttcg cgccgtcgac
    13381 tgttaacggc gatggcgctt tctccgttgt tatggcagat gaataccgcc cacgcggcgg
    13441 ctattgaccc caatcgtatt gtggcgctgg agtggttgcc ggtggaatta ctgctggcgc
    13501 tcggcatcgt gccttacggc gtggcggata ccatcaacta tcgcctgtgg gtcagcgaac
    13561 cgctattgcc ggactcagtg attgacgttg gtttgcgtac agaacctaac cttgaactgc
    13621 tgaccgaaat gaaaccgtcg tttatggtct ggtcggcagg atatggccct tcaccagaaa
    13681 tgctggcacg tattgcaccg ggtcgcggat ttaacttcag cgacggcaaa cagccgctgg
    13741 cgatggcgcg taaatcgctg acggaaatgg cagatttact taacctgcaa agcgcagcag
    13801 aaacgcattt agcgcaatat gaagacttta tccgcagcat gaaaccccgc tttgtgaagc
    13861 gtggtgcacg cccgttattg ctgacgacgc ttatcgatcc gcgccatatg ctggttttcg
    13921 gtccaaacag cttgtttcag gaaattctcg atgagtacgg catcccaaat gcctggcaag
    13981 gggaaaccaa cttctggggc agtaccgccg tcagtatcga tcgtctggcg gcgtataaag
    14041 acgttgatgt gctctgtttt gatcacgaca acagcaaaga catggatgcg ctaatggcaa
    14101 cgccgctgtg gcaggccatg ccgtttgtcc gcgccggacg ctttcagcgc gtacctgcag
    14161 tctggtttta tggtgcgacg ctctcggcaa tgcactttgt gcgcgttctg gataacgcca
    14221 tcggaggtaa agcgtgagta aacgaattgc gctttttccg gcgttattgc tggcgctgtt
    14281 ggtgattgcc gctacggcgc tcacctggat gaatttttcg caggcgctgc cgcgcagcca
    14341 gtgggcgcag gccacctggt cgccggatat tgacgtcatc gagcagatga tttttcacta
    14401 cagcttgttg ccgcgtctgg cgatttcgct gctggtgggc gcgggcctgg ggctggtggg
    14461 cgtgctgttt cagcaagtgc tgcgtaaccc gctggcggag ccgacgacgc ttggcgttgc
    14521 tacaggcgcg caactgggaa ttaccgtcac cacactttgg gcgatcccag gtgctatggc
    14581 gagccagttt gccgcgctgg tgggcgcttg tgtcgttggc ttaatcgtct ttggcgtcgc
    14641 gtgggggaaa cggctttcgc cggttacgct tattctcgcg gggctggtgg tgagccttta
    14701 ttgcggggca attaatcagc tactggttat cttccatcac gaccagctgc aaagcatgtt
    14761 cctgtggagc accggaacgc tgacgcaaac cgactggggc ggcgttgagc gtttatggcc
    14821 gcagttgctg ggcggcgtga tgttgacgtt attgctactt cgcccgttaa ccctgatggg
    14881 gattgatgat ggcgtggcgc gcaatctcgg gctggccttg tcgctcgcgc gtctggcagc
    14941 gctgtcgctg gcgattgtca tcagtgcgct gctggtgaac gctgtgggga ttatcggctt
    15001 tatcgggttg ttcgcgccgc tgctggcaaa aatgctgggg gcgcggcgtc tgctgccacg
    15061 actgatgctg gcgtcgctga ttggggcgct gatcctctgg ctttccgatc aaatcatcct
    15121 ctggctgact cgcgtgtgga tggaagtgtc caccggttcg gtcactgcgt tgatcggtgc
    15181 gccgctgcta ctgtggctgt tgccgcgttt acgcagcatt agcgcgccgg atatgaaggt
    15241 caacgatcgt gtcgcggctg aacgccaaca tgtgctggcg tttgccctcg cgggcggcgt
    15301 gctgctgttg atggctgtgg tggtggcgct gtcgtttggt cgtgatgcgc acggctggac
    15361 gtgggcgagc ggggcgttgc ttgaggattt gatgccctgg cgctggccgc gaattatggc
    15421 ggcgctgttt gcgggcgtca tgctggcggt ggcgggctgt attattcagc ggctgaccgg
    15481 aaacccgatg gcaagcccgg aagtgctggg gattagctct ggcgcagcgt ttggcgtggt
    15541 gttgatgctg tttctggtgc cgggtaatgc ctttggctgg ctgttacctg caggcagtct
    15601 cggcgcggcg gtgacgctgt tgatcattat gatcgccgcc ggccgcggtg gattttcccc
    15661 acaccgcatg ttactggcgg ggatggcgtt aagcaccgcg ttcaccatgc ttttgatgat
    15721 gttgcaggca agtggtgacc cgcgaatggc gcaagtgctg acctggattt ccggttcgac
    15781 ctacaacgcg accgatgcgc aggtctggcg cacgggaatt gtgatggtga ttttgctggc
    15841 gattaccccg ctgtgccgcc gctggctgac cattttaccg ctgggtggtg ataccgcccg
    15901 agccgtagga atggcgctga cgccgacgcg aattgcgctg ctgctgttag cggcttgcct
    15961 gacggcgacc gcgacgatga ctattggacc gttgagtttt gttggtttaa tggcaccgca
    16021 tattgcgcgg atgatgggct ttcgacggac gatgccacac atcgtaattt cggcgctggt
    16081 gggtggttta ctgctggtgt tcgctgactg gtgtgggcgg atggtgctgt ttccattcca
    16141 gatcccggca gggctgctgt cgacctttat cggcgcgcca tattttatct atttgttgag
    16201 aaagcagatc cgttaataga atgcctcgcc cggtgggcga ggcaaaatca tcacaacttc
    16261 gcaaacaccc gtcgtgcagc atcgatggtg ttattgatat cttccatgct gtgcgccacg
    16321 gacataaagc ccgcttcaaa cgctgacggt gccaggtaaa caccttcgtc cagcatcata
    16381 tggaagaaac gcttaaagcg ttccacgtca caggccatca catcctgata gcacgtcacg
    16441 gactcggcgt cggtaaagaa aataccgaac atgccgccaa cgtggttaac gaccagcgga
    16501 gttccggctt cttctgccgc ttccagcaga ccttctgcca gacgtgatgt cagctcatcc
    16561 aacgtttcgt gaacgcccgg ctgcgcgact tcattcagac aggcgaaacc cgctgccatc
    16621 gcaattgggt taccggaaag cgtacccgcc tgatagaccg gacccgtcgg ggccagcgca
    16681 tccattacat cacgacgacc accgaatgcg cctaccggca ttccaccgcc gatgattttg
    16741 ccgaggcagg ttaaatctgg cactacgccg taataatcct gtgcgccagc tagcgctacg
    16801 cggaaaccgg tcatcacttc atcgatgatc agcaacgcgc caaattcgtc gcacagcgcg
    16861 cgcagacctg gcaggaactc tggcagcggc ggaacacagt tcatattgcc tgccaccggc
    16921 tcgacgataa tacaggcaat ctcttgcggg tattgctcaa atgcggcgcg tacagaagcc
    16981 agatcattat aagtacaggt taaggtatgt ttggcgaaat ctgccggaac gcccggcgag
    17041 tttggctggc ctaacgtgag tgcgccagaa ccggctttca ccagcaggca gtcagcgtga
    17101 ccatggtaac acccttcaaa tttaataatt ttgtcgcgac cggtaaaacc acgggccagg
    17161 cggatggcgc tcatggtcgc ttcagtgccg gagttcacca tgcgcaccat atccatggtc
    17221 gggaccagtt cggtcaccag ttgcgccatt ttcacttcca tttcggttgg tgcaccaaag
    17281 cttaaaccac gctcggcggc ttcaatcacg gcattgcgga ttgccggatg gttatggccc
    17341 agcaccatcg gcccccagga accgacataa tcgatatagg ctttgccatc aacatcgtac
    17401 agataagcgc cgtccgcttt ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg
    17461 cgaacagggg agttcacacc gccagggatc agctcgcgcg ctgcgctgta aagattttca
    17521 gacttactca tggagggttc ctgattcgta gaaaaagtga atggctgcta ttctatgtta
    17581 ttcataacaa gttaaatact cgtcaaacat caggctgctt gtactggtgc aatcctgatt
    17641 tcttagagta taaaagtttt gtgcatttga aacattacgc tttgcaaagg attttcatgg
    17701 aacgtgcgag taaaatgccg tcatcttatt tgtatgacca ataagtgatc attggatgaa
    17761 aactgatact ccctctttag aaacaccgca ggccgcgcgc ctgcgacgca gacaactgat
    17821 tcgccaactt cttgagcgcg ataaaacccc gttagccatt ttgtttatgg cggcagtcgt
    17881 cggcacgctt gtcgggctgg cagcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa
    17941 ccaacgtatg ggggcgctgg tacatactgc tgataattat ccgcttctgt taaccgtcgc
    18001 ttttctctgt tcggcggtgc tggcgatgtt cggctacttt ttggtgcgca aatacgcgcc
    18061 ggaagcaggt ggttcgggga tcccggaaat tgaaggggcg ctggaagatc aacgtcccgt
    18121 tcgctggtgg cgtgtgttgc cggtgaaatt ctttggcggg ctggggacac tcggcggagg
    18181 catggtgcta gggcgcgaag gaccaaccgt gcagatcggc ggcaatatcg gtcgcatggt
    18241 gcttgatatt ttccgcctga aaggtgatga agctcgccat acgctgctgg caaccggtgc
    18301 tgctgcgggg ctggctgcgg cctttaacgc gccgctggcg ggtattttgt ttattatcga
    18361 agagatgcgt ccgcagtttc gctatacgtt aatttcgatt aaagcggtat ttattggcgt
    18421 cattatgtcg accattatgt accggatttt taatcatgaa gttgcgttga ttgacgtcgg
    18481 taaactttct gatgcgccgc ttaatacgct gtggctttat ctgatcctcg gtattatttt
    18541 tggcattttc ggccctattt ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg
    18601 tgtgcacggc ggcaatatta ccaaatgggt actgatgggt ggtgcgattg gcggtctgtg
    18661 tggattgctg gggtttgtgg caccagcaac gtcgggcggc ggttttaacc tgattcctat
    18721 cgctaccgcg gggaatttca gcatgggaat gctggtgttt atcttcgtcg ctcgggtcat
    18781 taccacctta ctctgcttct cttccggcgc gccgggcggt atttttgccc cgatgctggc
    18841 gctgggtact gtgctgggaa ccgctttcgg aatggttgcc gttgagctgt ttccgcaata
    18901 tcacctggag gcggggacgt ttgctattgc cggaatgggg gcattactgg cggcatctat
    18961 tcgcgcgccg ttaacgggga tcattctggt tctggagatg accgataact accagctcat
    19021 tttgccaatg attattaccg gtcttggcgc aacactatta gcgcaattta ccggcgggaa
    19081 accgctatac tcggcgattc ttgcgcgcac gctggcaaaa caggaagctg agcaactggc
    19141 gcgaagcaag gccgcatcag ccagcgagaa tacttgaacg aaataccagg gtattagata
    19201 atggcgatta ttattgggtt agaatttgcc caattgccga tgtcgtttgg agcaaaatat
    19261 gagtgatgac gtagcactgc cgctggagtt taccgacgca gcagccaaca aagttaaaag
    19321 cctgatcgct gacgaagata acccgaatct gaaattacgc gtgtatatca ccggtggcgg
    19381 ttgcagcggc ttccagtatg gtttcacctt tgatgatcag gtgaacgaag gcgatatgac
    19441 catcgaaaaa cagggcgttg gcctggtggt tgatccgatg agcctgcaat atctggtcgg
    19501 cggttccgtt gactacaccg aaggactgga aggttctcgt ttcatcgtga ccaacccgaa
    19561 cgcgaaaagc acctgcggtt gcggttcttc ctttagcatc taatctgttg ttcatgtgcc
    19621 ggataaacta tccggcacat cttctctctt caacgcccat tctcatccag cgcaaacgtc
    19681 ggtagcttaa gatgccaacg aatagccgcc agccgaatca atagcgtcac gaccatgccc
    19741 atcatactgg ctgtttccag tggtacggaa aatgtgtaat aagccgtagc gtggacaata
    19801 ccgccgataa tacaggcagt tgcgtagatt tctgtacgta aaatcatggg gatctcgcgg
    19861 gccagtacat cacgaatgat cccgccgcca acgccagtaa tgacgcccat gcaaaccgcg
    19921 attaacggac cggcttccgc attaaaggct ttattcacgc caatgccgac aaacaccgcc
    19981 agaccaacgg cgtccagcac cggcaacatc cattttggta agcgtcttgg ctggcgcacc
    20041 agcacgatgg tcagcatgct ggtgaccatt gcaacgacca gatcggtggg atctttcacc
    20101 caaaataccg ggccgtgatc cagcgccatg tcgcgaattg tcccgccgcc tactgcggtt
    20161 accacgccca gtaccagaac accaaaaggg tccatacgca attttccggc taacaaaacg
    20221 ccggagatgg caaataccgc tgtgccgact atatccagcc aatagacgag cattgttcaa
    20281 tccccactga gcacctgaaa aggtcaggcg ctaatctacc tgtgaaagcg cattacagag
    20341 ctgttgtgca gcgaggataa tacgtgggct tgcacgttca aaccagtcac tcgtgagagg
    20401 aataacggga attttgagct gttcacccca gtattgtttg attttaggaa tttggtccgg
    20461 tccgcctgta atgacaatcg cctgtggcga gcgtgctaac acctgttcgc ggctaacttg
    20521 cggccaggga acccggctgt ctttaaagat gttttctccg ccacaaactt cgagtacctg
    20581 gttctgaatc gactcttttc cactggtaaa tggcggatta atgccgaatt gcagaaaaac
    20641 acgtttttta ggtttatcag catattgcgc tttcaattgc gcgtactgat cctgcaggga
    20701 ttgcgcggct tgttcggcct tgtctggttg cggactccag ggggccagtt gacgtaacgc
    20761 attggcaatt tgttcaatgc ttgtcgcatc gacccacatc acttttattc ccagcgaagc
    20821 cagctggtca acctgccgct cggcattacc tccacgccag gcaatcacca gatcgggttt
    20881 cagcgcgaca atgcgttcca gattcatccc ctgccaggtg gaaacctgct caatcttttg
    20941 cgcttgtgga ggatagtcgg aatagctgct gaccccaacc ggcgtgatcc cggcggcaaa
    21001 ggcaagttca gtgttggcgg gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag
    21061 tggcgcaaga aaagacaggg cgaccagcgc cctgaacagt gacttagcca tgtgcaagtt
    21121 tctgcaccag tgactcaacc atcaggctgg actgtttagc ggcaacagcc aggaactcat
    21181 cgaagctaag atgagactgt tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg
    21241 ggacgttgaa attgtggcag acatgggcga ttgccgtcgc ttccatctct acagcaatgg
    21301 cctgtgggaa gttgtggcgg attttcgcca gaccaacaga accgttgatg aaagcgtcgc
    21361 cgctaacaat caggccacgt acagcgttaa gattcagttc ggcaatgcag gcctcagcgg
    21421 cagcgatcag tttatcgtca gctttaaagc ctgccggaca gcctggtaac tgaccgtatt
    21481 cataaccaaa tgccgtgaca tccgcgtcgt gataacgtgc ttcgtccgag acaacgatat
    21541 cgcccacttt cagcgttggt gccaggccac cggcagaacc ggtgttaata atcacatctg
    21601 gcttgcagtg ttccaacagc aaagtggcac ccagcgccgc agcgacttta ccgatgcccg
    21661 atttcagaag cgcaacctcg gttccattca gttggccggt atagatttcg caaccgccca
    21721 gactgatagt ttgacggttt tcgattttgt cacgcagcag cgtaacttct tcttccattg
    21781 caccaatgat gccgattttc atagatttac tcgcgataag cccgatttga aggcatagtt
    21841 taccatgcgc ttacggggaa gcgtatttct cacgcgggag aggacatggc acagattgat
    21901 ttccgaaaaa aaataaactg gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa
    21961 catgagatcc tgcggatctt cgagagcgat cgcgggcgta tcatcaactc tccggcaatt
    22021 cgtcgtctgc aacaaaagac gcaggttttt ccactggagc gcaatgccgc cgtgcgcacg
    22081 cgtcttaccc actcgatgga agtccagcag gtggggcgct acatcgccaa agaaatttta
    22141 agccgtctga aagagcttaa attgctggaa gcatacggcc tggatgaact gaccggacct
    22201 tttgaaagca ttgttgagat gtcatgcctg atgcacgata tcggcaatcc gccgtttggt
    22261 cattttggcg aagcagcgat aaatgactgg tttcgccaac gtttgcaccc ggaagatgcc
    22321 gaaagccagc ctctgactga cgatcgctgc agcgtggcgg cactacgttt acgggacggg
    22381 gaagaaccgc ttaacgagct gcggcgcaag attcgtcagg acttatgtca ttttgagggg
    22441 aatgcacaag gcattcgtct ggtgcataca ttgatgcgga tgaatctcac ctgggcacag
    22501 gttggcggta ttttaaaata tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca
    22561 catcactatt taatgaaaaa gccgggttat tatctttctg aagaagccta tattgcccgg
    22621 ttgcgtaaag aacttaattt ggcgctttac agtcgttttc cattaacgtg gattatggaa
    22681 gctgccgacg atatctccta ttgtgtggca gaccttgaag atgcagtaga gaaaagaata
    22741 tttaccgttg agcagcttta tcatcatttg cacgaagcgt ggggccagca tgagaaaggt
    22801 tcgctctttt cgctggtggt tgaaaatgcc tgggaaaaat cacgctcaaa tagtttaagc
    22861 cgcagtacgg aagatcagtt ttttatgtat ttacgggtaa acaccctaaa taaactggta
    22921 ccctacgcgg cacaacgatt tattgataat ctgcctgcga ttttcgccgg aacgtttaat
    22981 catgcattat tggaagatgc cagcgaatgc agcgatcttc ttaagctata taaaaatgtc
    23041 gctgtaaaac atgtgtttag ccatccagat gtcgagcagc ttgaattgca gggctatcgg
    23101 gtcattagcg gattattaga gatttatcgt cctttattaa gcctgtcgtt atcagacttt
    23161 actgaactgg tagaaaaaga acgggtgaaa cgtttcccta ttgaatcgcg cttattccac
    23221 aaactctcga cgcgccatcg gctggcctat gtcgaggctg tcagtaaatt accgtcagat
    23281 tctcctgagt ttccgctatg ggaatattat taccgttgcc gcctgttgca ggattatatc
    23341 agcggtatga ccgacctcta tgcgtgggat gaataccgac gtctgatggc cgtagaacaa
    23401 taaccaggct tttgtaaaga cgaacaataa atttttacct tttgcagaaa ctttagttcg
    23461 gaacttcgcg ctataaaacg aatctgaaga acacagcaat tttgcgttat ctgttaatcg
    23521 agactgaaat acatgaaaaa aaccacatta gcactgagtg cactggctct gagtttaggt
    23581 ttggcgttat ctccgctctc tgcaacggcg gctgagactt cttcagcaac gacagcccag
    23641 cagatgccaa gccttgcacc gatgctcgaa aaggtgatgc cttcagtggt cagcattaac
    23701 gtagaaggta gcacaaccgt taatacaccg cgtatgccgc gtaatttcca gcagttcttc
    23761 ggtgatgatt ctccgttctg ccaggaaggt tctccgttcc agagctctcc gttctgccag
    23821 gggggccagg gcggtaatgg cggcggccag caacaaaaat tcatggcgct gggttccggc
    23881 gtcatcattg atgccgataa aggctatgtc gtcaccaaca accatgttgt tgataacgcg
    23941 acggtgatta aagtccagct gagcgatggt cgtaagttcg acgcgaagat ggttggcaaa
    24001 gatccgcgct ctgatatcgc gctgatccaa atccagaacc cgaaaaacct gaccgcaatt
    24061 aagatggcgg attctgatgc actgcgcgtg ggtgattaca ccgtagcgat tggtaacccg
    24121 tttggtctgg gagagacggt aacttccggg attgtctctg cgctggggcg tagcggcctg
    24181 aatgccgaaa actacgaaaa cttcatccag accgatgcag cgatcaaccg tggtaactcc
    24241 ggtggtgcgc tggttaacct gaacggcgaa ctgatcggta tcaacaccgc gatcctcgca
    24301 ccggacggcg gcaacatcgg tatcggtttt gctatcccga gcaacatggt gaaaaacctg
    24361 acctcgcaga tggtggaata cggccaggtg aaacgcggtg agctgggtat tatggggact
    24421 gagctgaatt ccgaactggc gaaagcgatg aaagttgacg cccagcgcgg tgctttcgta
    24481 agccaggttc tgcctaattc ctccgctgca aaagcgggca ttaaagcggg tgatgtgatc
    24541 acctcactga acggtaagcc aatcagcagc tttgccgcac tgcgtgctca ggtgggtact
    24601 atgccggtgg gcagcaaact gaccctgggc ttgctgcgcg acggtaagca ggtcaacgtg
    24661 aatctggaac tgcagcagag cagccagaat caggttgatt ccagctccat cttcaacggc
    24721 attgaaggtg ctgagatgag caacaaaggc aaagatcagg gcgtggtagt gaacaacgtg
    24781 aaaacgggca ctccggctgc gcagatcggc ctgaagaaag gtgatgtgat tattggcgcg
    24841 aaccagcagg cagtgaaaaa catcgctgaa ctgcgtaaag ttctcgacag caaaccgtct
    24901 gtgctggcac tgaacattca gcgcggcgac agctccatct acctgttaat gcagtaatct
    24961 ccctcaaccc cttcctgaaa acgggaaggg gttctcctta caatctgtga acttcaccac
    25021 aactccatac atcttcatca tcctttaggc atttgcacaa tgccgtacgt tacgtacttc
    25081 cttatgctaa gccgtgcata acggaggact tatggctggc tggcatcttg ataccaaaat
    25141 ggcgcaggat atcgtggcac gtaccatgcg catcatcgat accaatatca acgtaatgga
    25201 tgcccgcggg cgaattatcg gcagcggcga tcgtgagcgt attggtgaat tgcacgaagg
    25261 tgcattgctg gtactttcac agggacgagt cgtcgatatc gatgacgcgg tggcacgcca
    25321 tctgcacggt gtgcggcagg ggattaatct accgttacgg ctggaaggtg aaattgtcgg
    25381 cgtaattggc ctgacaggtg aaccagagaa tctgcgtaaa tatggcgaac tggtctgcat
    25441 gacggctgaa atgatgctgg aacagtcgcg gttgatgcac ctgttggcgc aggatagccg
    25501 tttgcgggaa gaactggtga tgaacctgat tcaggctgag gagaatactc ccgcacttac
    25561 tgaatgggcg caacggctgg ggatcgatct caatcaaccg cgagtagtgg ctattgttga
    25621 ggtcgacagc ggtcagcttg gcgtggacag cgcaatggcg gagttacaac aactgcaaaa
    25681 tgcgctgact acgcccgagc gtaataatct ggtggcgatt gtctcgctga ccgaaatggt
    25741 ggtgttgaaa ccggcgttga actcttttgg gcgctgggat gcagaagatc atcgtaagcg
    25801 agttgaacaa ctgattaccc gcatgaaaga gtatggccag ctgcgttttc gcgtttcgct
    25861 gggcaactat tttaccggtc ctggcagtat tgcccgatcc tatcgtacgg cgaaaacgac
    25921 gatggtggtg ggtaaacagc ggatgccaga aagtcgctgc tatttttatc aggatctgat
    25981 gttacctgtg ttactcgaca gtttgcgtgg cgactggcag gccaatgaac tggcgcgacc
    26041 gctggcgcgg ctgaaagcga tggacaataa tggcttgctg cgacgaacgc tggcggcgtg
    26101 gtttcgccac aatgtgcaac cgctggctac gtcaaaggcg ctgtttattc atcgtaatac
    26161 tctggagtat cggcttaatc gtatatcgga actgaccggg cttgatttgg gtaattttga
    26221 tgacaggttg ctgctgtatg tggcgttgca gctggatgaa gagcggtagg ttatgcgtta
    26281 aggtcgaggc ggcgctggct cattcccctc agcccgaagg gtagagggga atgtccgagt
    26341 taaccgtcag ttcggcatta tttacgcgtt agtttttcca gatcggcttc aatctcgctg
    26401 atcttattgg ttacgactga ctccaggtga cgtaagtcgt cgaggatctt acgcttaaga
    26461 tcaacttcgc tgcggtcacg ctggcagatt tgatcaagct catcaatgat ataccgtaga
    26521 ttcgggctga tttcctggac ttctttataa ccctgaccca caccatcagc gacgaccgtt
    26581 ttacgctgac gcggatattt aaacttaacg ctcttggcga aaaactcgcc tttgtctttc
    26641 tggaaataga ttttcagaat atcgttgttg gcttcctgcc ggaggctgta acgatcaatt
    26701 tcttcaggat tggtaatacc cagacttttc agattgtcgt acatagcgtt acctcaaaat
    26761 gagtcagtaa attgtgctta ttttagcatt tggcctggcc cgcccggctc gactgtgatc
    26821 gcagaaagcc tttttcttcc agacttctat catggcgtaa aaatcaaaaa attacctgct
    26881 ttattgtggc gatattccat gggatggatt aacacattta tcttcgtttt gcgaattaca
    26941 tgacagtccg aaacaagatg aaaaagtatt taaaaaaggg gggaagaaga atgagtagga
    27001 cgaattattc tgagccagaa agcatacggg cagcatcatg ctgcccgtat atgtactctt
    27061 aatcgatggt acgcagcagt tcgttaatgc cgactttgcc gcgagttttc gcgtcaactt
    27121 tcttaacgat aaccgcacag tagaggctgt atttgccatc tttcgacggc agattacctg
    27181 aaacaaccac agaccccgcc ggaacgcgac cgtagtggat ttcgccggtt tcacggtcgt
    27241 aaatacgggt gctctgacca atgtatacgc ccatagagat gacggaacct tcttcgacaa
    27301 tcaccccttc aaccacttcg gagcgtgcgc caatgaagca gttatcttca ataatggtcg
    27361 ggttagcctg cagcggctcc agcacgccgc cgatgcccac gccaccggaa aggtggacgt
    27421 ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt atcaaccatc gtgccttcat
    27481 caacatatgc gccgatgttg acgtaagacg gcatcagcac ggtgttacgg gcaataaatg
    27541 caccctgacg taccgccgct ggtggcacaa cgcggaagcc ttctttctga aaacgcgctt
    27601 cgtcgtagtc ggcgaatttc atcggcactt tatcgaagta gcggctttct gccccttcga
    27661 tcacctgatt atcattaata cggaaagaga gcagcaccgc ttttttcaac cactgatgcg
    27721 tcacccactg accgtcaatt ttttccgcta cacgcagtgc gccggaatcc agcagggcga
    27781 tcacctgatt taccgcttcg cgggttacgg tgtctgcatt ggctggcgtg atctcggcac
    27841 ggcgttcaaa agcggtttca ataatgttct gtaactgctg cattgttaaa ctcttttcat
    27901 atcagtaaac acatcaccct ttatcgtttg gattgagggc ctctgtcaac cgctgatgca
    27961 cttcctgctg caactcgtta ttaagtgcac gccggtcagc ggtggcaatt atgaataaat
    28021 cttctactcg ctcgccaatg gttgtaattc gggcaccatg aagcgaaatt cccagatcgg
    28081 caaaaatttt cccgactctc gccagcagtc ctggctggtc gagcgcgata agctcaagga
    28141 acgatttgcg gtcggtatgg gtcggcaaaa acgttacttc ggtttcaaca gtaaaatggc
    28201 gtaatttggc gggttggcga cggggctgcg gtggctgcca gctactttgc gtcagtactt
    28261 gctccagacc aaaccgaata acctcatgac gatctgcgga cagcgggctg ccatcgggtt
    28321 ccagcacgat aaaggtatcc atcgccatac cgtcgcgagt ggtgaaaatt tgtgcgtcgt
    28381 gaacacttaa attgcggcgg tctaattcgg cacagacggc ggcaaacaga taggggcggt
    28441 ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc ctgcgggcta agcaatacca
    28501 gcggtttgct taaatcatgc tgtaataaat ggcgggcatg ccaggccagt tgatttgggc
    28561 tatggcggac aaaatagtta gcacgacagc gtgaccaaat ttggtgcagc gcctcttcgt
    28621 cgatgttatc catgcgcagt agtgccagtg cctggagttg gtgatggcga acccgttcgc
    28681 gcatatccgg cgtgttttgc attccgcgtc gtagctgctt ttcggtggca aaatagagtt
    28741 cgcgcaacag actttgcttc cagctattcc acagcgtttc gttggtggcg caaatgtcag
    28801 ccactgtcag gcataccaga tagcgcagac gattttccgt ttgaacttct tcggcaaact
    28861 gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc tgtcaccgac atcagcaagt
    28921 gctggcgaac cagccaggca accagctgtg tttcgcgtga gttcagcccg tggagttctg
    28981 caaaatgcac tacatcctga gcaccgagaa tggagtggtc gccgccgcgt cctttggcga
    29041 tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga cggcaggcgc ggccagacgt
    29101 ccacacacaa cggatggcgc tggcgcgttt cttcactggc aaaactctcc agtttcagca
    29161 tcacgcggat agtatgttca tccaccgtgt aggcgtggaa cagatcaaac tgcatctgcc
    29221 cgacgatatg cgaccattgc ggcatatacg cgccgagcac gctatggcga tgcattggca
    29281 atagcccgcg ccgcaccgct ccggggtgac gcagaatgct caaaaacagt tttcgtgctt
    29341 ccggaatatt acacagcggt tgttgcagat ggcgacgggc atggcgtaac tggcgcagcg
    29401 tggtggagta aatgccggtg atcgcactgt tgcgcaccat ggtgtagaac atacgcaaga
    29461 tggcttccgg ctggcgcata aatagtgttt catcacgcag gtcgattagc gtaccgcgta
    29521 gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc gggaagggcg aggatggctt
    29581 catcgaacag ttgcagcagc atctggttga gttcactgac gcggcgtgta acgcggaagt
    29641 aatccttcat catccgctcg accggttcgt tgccttcacc actgtaattc agacgctgag
    29701 cgacgctaag ttggcgatcg aataacagac gattatcgta acggctgacg accagatgca
    29761 gggcaaagcg aatacgccac agtatatgca gacattcgtt taattccgcc cgctccgctg
    29821 aggttaagaa gccaaacccg accatttcat ccagcgatgt tgcgccaaaa tgacggcggg
    29881 ccacccattg cagagtgtga atatcgcgca agccgccagg gctacttttg atgtctggtt
    29941 caaggttgta gctggtgcca tggtaacgct gatggcgctg gttctgttct tcaactttcg
    30001 ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa aatatgtttt tgcagttcga
    30061 ggaagagcgc aacatcgcca attaataagc gggattcgat taaattggtg gcgacggtta
    30121 aatccgataa cccttccagc atgcactctt caagcgtgcg cacactatga ccgacttcca
    30181 gctttacatc ccagagcagc gttaacagct cgcccacttt ttgcgcctga tcgtccggga
    30241 gctttttacg gcttaaaatc agtaaatcga tgtcagaaag tggatgcagc tcgccacgac
    30301 cgtagccacc gacggcgacc aatgccaggt cggcaatctg gctgaatccc gcttcaatcc
    30361 ataatcgttg caggagctgg tcgataaact cggtgcgcgc ctcaatcaac tgttctgcag
    30421 agatcccatt gtcaaaggca tcacccagcc aacgctggaa agtatcgata tgggctttta
    30481 tcccaccgac ggttaattca tcacggggcc agacgcatgg attttgcggt tgaccgggca
    30541 gggtggggag agcggtgttt gcgtactgtt ctggaagggt attcattgtg cgccacccat
    30601 aagattaaat tatcgcatta aaaaagccgg cattcgccgg cttcatctta ttcgtcgtgc
    30661 gagattatcg ccgggatggt gtcatccttg cgtagcgtca gaatttcgca gccgttatca
    30721 gtcaccacaa tagtatgctc atattgtgca gacaagctgc gatctttggt ttttaccgtc
    30781 cagccatctt tcatggtgcg gatctctttt ttacccgcgt tgaccattgg ctcgatggtg
    30841 aacgtcatcc caggtttcag tacgacgttg gtttcacggg agtcatagtg cagcacctgc
    30901 ggttcttcat ggaagccgcg accaataccg tgtccgcaat attcacgaac gacggagaag
    30961 ccttctgctt cgacaaattt ctgaatcgcc gcaccgattt cgcgcagatt aatgcctggt
    31021 ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga tgcggcacag acgttcgccc
    31081 atgatggtcg gcttaccgac gataaacatt ttcgaggtat cgccgtggaa accatctttg
    31141 attacggtga catcaatgtt aacgatatcg ccatctttca gcagcttcgc atcgtccggg
    31201 ataccgtggc acaccacttc attaatagag atgcaaacgg atttcggata gccgtgatag
    31261 ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt aatcattaca gatgcgatcc
    31321 agctcgccgg tgctgacgcc cggtttaaca tacggttcga tcatctccag cacttcggca
    31381 gccagtcggc cagcgacgcg cattttttcg atatcttctg gggtcttgat tgagatagcc
    31441 attaattctg tccatcagcg tcggtgatac cgacaatata tatgtaagtg ccgtcaatgg
    31501 tatcacaccc gggcaaattg agaatcattc tgaatttcgc caaacgtgcc actgaacgtt
    31561 ttctataata gaaaattcaa cgtctgatgc tgtacacagc gccaacaatt attggtgtcc
    31621 atgacgtatt tgtggtataa agcgcgccgg acttccgatc catttcgtat acacagactg
    31681 gacggaagcg acaatctcac tttgtgtaac aacacacacg tatcggcaca tattccgggg
    31741 tgccctttgg ggtcggtaat atgggatacg tggaggcata accccaactt ttatatagag
    31801 gttttaatca tggcaactgt ttccatgcgc gacatgctca aggctggtgt tcacttcggt
    31861 caccagaccc gttactggaa cccgaaaatg aagccgttca tcttcggtgc gcgtaacaaa
    31921 gttcacatca tcaaccttga gaaaactgta ccgatgttca acgaagctct ggctgaactg
    31981 aacaagattg cttctcgcaa aggtaaaatc cttttcgttg gtactaaacg cgctgcaagc
    32041 gaagcggtga aagacgctgc tctgagctgc gaccagttct tcgtgaacca tcgctggctg
    32101 ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca tcaaacgtct gaaagacctg
    32161 gaaactcagt ctcaggacgg tactttcgaa aagctgacca agaaagaagc gctgatgcgc
    32221 actcgtgagc tggagaaact ggaaaacagc ctgggcggta tcaaagacat gggcggtctg
    32281 ccggacgctc tgtttgtaat cgatgctgac cacgaacaca ttgctatcaa agaagcaaac
    32341 aacctgggta ttccggtatt tgctatcgtt gataccaact ctgatccgga cggtgttgac
    32401 ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga ccctgtacct gggcgctgtt
    32461 gctgcaaccg tacgtgaagg ccgttctcag gatctggctt cccaggcgga agaaagcttc
    32521 gtagaagctg agtaataagg cttgataact cccccaaaat agttcgagtt gcagaaaggc
    32581 ggcaagctcg agaattcccg ggagcttaca tcagtaagtg accgggatga gcgagcgaag
    32641 acaacgcatc tgcggcgcga aatatgaagg gggagagccc ttatagacca ggtagtacac
    32701 gtttggttag ggggcctgca tatggccccc tttttcactt ttatatctgt gcggtttaat
    32761 gccgggcaga tcacatctcc gaggatttta gaatggctga aattaccgca tccctggtaa
    32821 aagagctgcg tgagcgtact ggcgcaggca tgatggattg caaaaaagca ctgactgaag
    32881 ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa gtccggtgct attaaagcag
    32941 cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa aaccaaaatc gacggcaact
    33001 acggcatcat tctggaagtt aactgccaga ctgacttcgt tgcaaaagac gctggtttcc
    33061 aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg caaaatcact gacgttgaag
    33121 ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt agcgaaaatt ggtgagaaca
    33181 tcaacattcg ccgcgttgct gcgctggaag gcgacgttct gggttcttat cagcacggtg
    33241 cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga agagctggtt aaacacatcg
    33301 ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc agaagacgta tccgctgaag
    33361 tggtagaaaa agaataccag gtacagctgg atatcgcgat gcagtctggt aagccgaaag
    33421 aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt caccggcgaa gtttctctga
    33481 ccggtcagcc gttcgttatg gaaccaagca aaactgttgg tcagctgctg aaagagcata
    33541 acgctgaagt gactggcttc atccgcttcg aagtgggtga aggcatcgag aaagttgaga
    33601 ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc ttaattatcg aaaaggagcc
    33661 gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc agctaaccct tgtggggctg
    33721 cgctgaaaag cgacgtacaa tgtcgctagt attaattcat ttcaatcgtt gacagtctca
    33781 ggaaagaaac atggctacca atgcaaaacc cgtctataaa cgcattctgc ttaagttgag
    33841 tggcgaagct ctgcagggca ctgaaggctt cggtattgat gcaagcatac tggatcgtat
    33901 ggctcaggaa atcaaagaac tggttgaact gggtattcag gttggtgtgg tgattggtgg
    33961 gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt atgaaccgcg ttgtgggcga
    34021 ccacatgggg atgctggcga ccgtaatgaa cggcctggca atgcgtgatg cactgcaccg
    34081 cgcctatgtg aacgctcgtc tgatgtccgc tattccattg aatggcgtgt gcgacagcta
    34141 cagctgggca gaagctatca gcctgttgcg caacaaccgt gtggtgatcc tctccgccgg
    34201 tacaggtaac ccgttcttta ccaccgactc agcagcttgc ctgcgtggta tcgaaattga
    34261 agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg tttactgctg atccggcgaa
    34321 agatccaact gcaaccatgt acgagcaact gacttacagc gaagtgctgg aaaaagagct
    34381 gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac cataaattac cgattcgtgt
    34441 tttcaacatg aacaaaccgg gtgcgctgcg ccgtgtggta atgggtgaaa aagaagggac
    34501 tttaatcacg gaataattcc cgtgatggat aaataagggt aagattccgc ataagtatcg
    34561 cgggggcgta agtctggtta taaggcgtta ttgttgcagg cagtttggtc acggccagcg
    34621 cgcagcaacc ggtgcgtaca caagtacgtg aggatggcga gcactacccg gggccaaaat
    34681 ggcaaataaa atagcctaat aatccagacg attacccgta atatgtttaa tcagggctat
    34741 acttagcaca cttccactgt gtgtgactgt ctggtctgac tgagacaagt tttcaaggat
    34801 tcgtaacgtg attagcgata tcagaaaaga tgctgaagta cgcatggaca aatgcgtaga
    34861 agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt gcttctccca gcctgctgga
    34921 tggcattgtc gtggaatatt acggcacgcc gacgccgctg cgtcagctgg caagcgtaac
    34981 ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat cgttcaatgt ctccggccgt
    35041 tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg aactctgcgg gtagcgacat
    35101 ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa gatctgacca aaatcgttcg
    35161 tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg cgtcgtgacg cgaacgacaa
    35221 agtgaaagca ctgttgaaag ataaagagat cagcgaagac gacgatcgcc gttctcagga
    35281 cgatgtacag aaactgactg atgctgcaat caagaaaatt gaagcggcgc tggcagacaa
    35341 agaagcagaa ctgatgcagt tctgatttct tgaacgacaa aaacgccgct cagtagatcc
    35401 ttgcggatcg gctggcggcg ttttgctttt tattctgtct caactctgga tgtttcatga
    35461 agcaactcac cattctgggc tcgaccggct cgattggttg cagcacgctg gacgtggtgc
    35521 gccataatcc cgaacacttc cgcgtagttg cgctggtggc aggcaaaaat gtcactcgca
    35581 tggtagaaca gtgcctggaa ttctctcccc gctatgccgt aatggacgat gaagcgagtg
    35641 cgaaacttct taaaacgatg ctacagcaac agggtagccg caccgaagtc ttaagtgggc
    35701 aacaagccgc ttgcgatatg gcagcgcttg aggatgttga tcaggtgatg gcagccattg
    35761 ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg cgcgggtaaa accattttgc
    35821 tggccaataa agaatcactg gttacctgcg gacgtctgtt tatggacgcc gtaaagcaga
    35881 gcaaagcgca attgttaccg gtcgatagcg aacataacgc catttttcag agtttaccgc
    35941 aacctatcca gcataatctg ggatacgctg accttgagca aaatggcgtg gtgtccattt
    36001 tacttaccgg gtctggtggc cctttccgtg agacgccatt gcgcgatttg gcaacaatga
    36061 cgccggatca agcctgccgt catccgaact ggtcgatggg gcgtaaaatt tctgtcgatt
    36121 cggctaccat gatgaacaaa ggtctggaat acattgaagc gcgttggctg tttaacgcca
    36181 gcgccagcca gatggaagtg ctgattcacc cgcagtcagt gattcactca atggtgcgct
    36241 atcaggacgg cagtgttctg gcgcagctgg gggaaccgga tatgcgtacg ccaattgccc
    36301 acaccatggc atggccgaat cgcgtgaact ctggcgtgaa gccgctcgat ttttgcaaac
    36361 taagtgcgtt gacatttgcc gcaccggatt atgatcgtta tccatgcctg aaactggcga
    36421 tggaggcgtt cgaacaaggt caggcagcga cgacagcatt gaatgccgca aacgaaatca
    36481 ccgttgctgc ttttcttgcg caacaaatcc gctttacgga tatcgctgcg ttgaatttat
    36541 ccgtactgga aaaaatggat atgcgcgaac cacaatgtgt ggacgatgtg ttatctgttg
    36601 atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg tctcgcaagc tgaggataat
    36661 ccggctacag agagtcgcgc tatttgttag cgtagggctt cagtgatata gtctgcgcca
    36721 tctgatcgta agtagttggc tttataaggt caggtatgcc gtggttttac acggcttttt
    36781 tttgtatagg cttcagtatt cctgagtacc gtaaaccctg tcagggaata aaaaacgcgt
    36841 gatgttgtct gctactcaac cacttagcga aaaattgcca gcgcatggct gccgtcatgt
    36901 tgcgatcatt atggacggca atggtcgctg ggcaaaaaag caagggaaga ttcgtgcctt
    36961 tgggcataaa gccggggcaa aatccgtccg ccgggctgtc tcttttgcgg ccaacaacgg
    37021 tattgaggcg ttaacgctgt atgcctttag tagtgaaaac tggaaccgac cagcgcagga
    37081 agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat agcgaagtaa aaagtctgca
    37141 ccgacataac gtgcgtctgc gtattattgg cgataccagt cgctttaact cgcgtttgca
    37201 agaacgtatt cgtaaatctg aagcgctaac agccgggaat accggtctga cgctgaatat
    37261 tgcggcgaac tacggtggac gttgggatat agtccaggga gtcaggcaac tggctgaaaa
    37321 ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa gagatgctaa accagcatgt
    37381 ctgtatgcat gaactggccc ctgtagattt agtaattagg actggggggg agcatcgcat
    37441 tagtaacttt ttgctttggc aaattgccta tgccgaactt tactttacag atgttctctg
    37501 gcccgatttc gatgaacaag actttgaagg ggcgttaaat gcctttgcta atcgagagcg
    37561 tcgtttcggc ggcaccgagc ccggtgatga aacagcctga tggggggcgc ttttgctgaa
    37621 gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc atcgcggcgt tgtttctgtt
    37681 gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc atgctggcag cgtgggaatg
    37741 gggacagctt agcggtttta ccactcgttc gcagcgagta tggttggcgg tgttatgcgg
    37801 gttattgttg gcgctgatgc tttttctgtt gccggaatat caccgaaata ttcatcaacc
    37861 gctggttgaa atctcacttt gggcttcgct gggttggtgg attgtcgcgc tattgctggt
    37921 gctgttttac ccaggttccg cagcaatctg gcgtaactct aaaacattgc gccttatttt
    37981 tggcgtgcta accattgttc ccttcttctg gggcatgctg gcgttacggg cctggcacta
    38041 tgacgagaat cattacagtg gcgcaatatg gctgctctat gtcatgatcc tggtatgggg
    38101 cgctgactcc ggcgcatata tgtttggcaa attgtttggt aaacataagc tggcaccgaa
    38161 ggtttctccg ggtaaaacct ggcaaggctt tataggtgga ctagcgaccg cagcggtaat
    38221 ctcatggggt tatggcatgt gggcgaatct cgacgtcgct cccgtcacct tactcatttg
    38281 ctctattgtc gcagcgttag cctcagtgct cggcgatctg accgagagta tgtttaagcg
    38341 tgaagcagga attaaggaca gcggtcattt aattccagga cacggtggta ttttagatcg
    38401 tattgatagc ctgacggctg cggtaccggt ctttgcttgc ttgttgttac tggtattcag
    38461 gacgctttaa cggaaggtaa tatgctgagt tttctctggg atttggcttc gttcatcgtt
    38521 gcactgggtg tacttatcac cgtgcatgaa tttggtcatt tctgggttgc ccggcgttgt
    38581 ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg cgctctggcg gcgaactgat
    38641 aagctcggca ccgaatatgt tatcgccctg atcccgctgg gcggttatgt caaaatgctg
    38701 gatgagcgcg cagaaccggt cgttccggaa ctccgccacc atgccttcaa taataaatct
    38761 gttggccaac gagcggcgat tattgccgca ggtccggttg caaacttcat ttttgctatc
    38821 tttgcctact ggctggtttt tattattggt gtgcctggcg tacgtccggt ggttggtgaa
    38881 atagcagcca attcgatagc tgcggaagca caaattgcac caggtacgga actaaaagcc
    38941 gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt tgcagttggt cgataaaatt
    39001 ggcgatgaaa gcaccaccat tacagtagcg ccatttggca gcgatcaacg gcgggatgta
    39061 aagctcgatt tacgtcactg ggcgtttgag cctgataaag aagatccggt atcttcgctg
    39121 gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg aaaatgtgca gccaaactcg
    39181 gcggcaagca aggcaggttt gcaagcaggc gacaggatcg ttaaagtcga tggtcagccc
    39241 ttaacgcagt gggtgacctt tgtgatgctt gtccgggata acccgggtaa atccttagcg
    39301 ttagaaatcg aaaggcaggg aagtcccttg tctttgacat taatcccgga gagtaaaccg
    39361 ggtaatggta aagcgattgg ttttgtcggt attgagccga aagtcattcc tttgccagat
    39421 gagtataaag ttgtacgcca gtatgggccg ttcaacgcca tcgtcgaagc cacggacaaa
    39481 acgtggcagc tgatgaagct gacggtcagt atgctgggaa aattgatcac cggtgatgtg
    39541 aaactgaaca acctcagtgg gccgatctct atcgccaagg gggctgggat gacagcggaa
    39601 ctcggggtag tttattacct gccgtttctt gcgcttatta gcgtgaactt agggataatt
    39661 aacctgtttc cgttgcccgt acttgacggg gggcatctgc tgttccttgc gatcgaaaag
    39721 atcaagggcg gaccggtatc cgagcgggtt caagactttt gttatcgcat tggctcgatt
    39781 ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt tctctcggtt atgagagtta
    39841 gttaggaaga acgcataata acgatggcga tgaaaaagtt gctcatagcg tcgctgctgt
    39901 ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt gaaagatatt catttcgaag
    39961 gccttcagcg tgtcgccgtt ggtgcggccc tcctcagtat gccggtgcgc acaggcgaca
    40021 cggttaatga tgaagatatc agtaatacca ttcgcgctct gtttgctacc ggcaactttg
    40081 aggatgttcg cgtccttcgt gatggtgata cccttctggt tcaggtaaaa gaacgtccga
    40141 ccattgccag cattactttc tccggtaaca aatcggtgaa agatgacatg ctgaagcaaa
    40201 acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga tcgcaccacc attgccgata
    40261 tcgagaaagg tctggaagac ttctactaca gcgtcggtaa atatagcgcc agcgttaaag
    40321 ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa actggtgttc caggaaggtg
    40381 tgtcagctga aatccagcaa attaacattg ttggtaacca tgctttcacc accgacgaac
    40441 tgatctctca tttccaactg cgtgacgaag tgccgtggtg gaacgtggta ggcgatcgta
    40501 aataccagaa acagaaactg gcgggcgacc ttgaaaccct gcgcagctac tatctggatc
    40561 gcggttatgc ccgtttcaac atcgactcta cccaggtcag tctgacgcca gataaaaaag
    40621 gtatttacgt cacggtgaac attaccgaag gcgatcagta caagctttct ggcgttgaag
    40681 tgagcggcaa ccttgccggg cactccgctg aaattgagca gctgactaag atcgagccgg
    40741 gcgagctgta taacggcacc aaagtgacca agatggaaga tgacatcaaa aagcttctcg
    40801 gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc tgaaattaac gatgccgaca
    40861 aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg tttctacgtg cgtaagatcc
    40921 gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg tcgcgaaatg cgtcagatgg
    40981 aaggtgcatg gctgggaagc gatctggtcg atcagggtaa ggagcgtctg aatcgtctgg
    41041 gcttctttga aactgtcgat accgataccc aacgtgttcc gggtagcccg gaccaggtag
    41101 atgtcgtcta caaggtaaaa gagcgcaaca ccggtagctt caactttggt attggttacg
    41161 gtactgaaag tggcgtgagc ttccaggctg gtgtgcagca ggataactgg ttaggtacag
    41221 gttatgctgt tggtatcaac gggaccaaaa acgattacca gacctatgct gaactgtcgg
    41281 taaccaaccc gtacttcact gtagatggtg taagcctcgg tggtcgtctc ttctataatg
    41341 acttccaggc agatgacgcc gacctgtccg actataccaa caagagttat ggtacagacg
    41401 tgacgttggg cttcccgatc aacgaatata actcgctgcg tgcaggtctg ggttatgtac
    41461 ataactccct gtccaacatg cagcctcagg ttgcgatgtg gcgttatctg tactctatgg
    41521 gtgaacatcc gagcacctct gatcaggata atagcttcaa aacggacgac ttcacgttca
    41581 actatggttg gacctataac aagcttgacc gtggttactt cccgacagat ggttcacgtg
    41641 tcaacctgac cggtaaagtg accattcctg gatcggataa cgaatactac aaagtgacgt
    41701 tagacacggc gacttatgtg ccgatcgatg acgatcacaa atgggttgtt ctggggcgta
    41761 cccgctgggg ttatggtgat ggtttaggcg gcaaagagat gccgttctac gagaacttct
    41821 atgccggtgg ttccagcacc gtgcgtggct tccagtccaa tacaattggt ccgaaagcag
    41881 tttacttccc gcatcaggcc agtaattatg atccggacta tgattacgaa tgtgcgactc
    41941 aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt aggcggtaac gccatggcgg
    42001 ttgccagcct cgagttcatc accccgacgc cgtttattag cgataagtat gctaactcgg
    42061 ttcgtacttc cttcttctgg gatatgggta ccgtttggga tacaaactgg gattccagcc
    42121 aatattctgg atatccggac tatagtgatc caagcaatat ccgtatgtct gcgggtatcg
    42181 cattacaatg gatgtcccca ttggggccgt tggtgttctc ctacgcccag ccgttcaaaa
    42241 agtacgatgg agacaaggca gaacagttcc agtttaacat cggtaaaacc tggtaagtgt
    42301 tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt aggcgatcaa tataagatcg
    42361 ccgggccacg caaagaactg caccctccgg tgcaaatggg atggtaagga gtttattgtg
    42421 aaaaagtggt tattagctgc aggtctcggt ttagcactgg caacttctgc tcaggcggct
    42481 gacaaaattg caatcgtcaa catgggcagc ctgttccagc aggtagcgca gaaaaccggt
    42541 gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca gcgaactgca gcgtatggaa
    42601 accgatctgc aggctaaaat gaaaaagctg cagtccatga aagcgggcag cgatcgcact
    42661 aagctggaaa aagacgtgat ggctcagcgc cagacttttg ctcagaaagc gcaggctttt
    42721 gagcaggatc gtgcacgtcg ttccaacgaa gaacgcggca aactggttac tcgtatccag
    42781 actgctgtga aatccgttgc caacagccag gatatcgatc tggttgttga tgcaaacgcc
    42841 gttgcttaca acagcagcga tgtaaaagac atcactgccg acgtactgaa acaggttaaa
    42901 taagtaatgc cttcaattcg actggctgat ttagcgcagc agttggatgc agaactacac
    42961 ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc aatctgcaca aacaggtcac
    43021 attacgttca tggttaaccc aaaataccgt gagcatttag gcttgtgcca ggcgtccgcg
    43081 gttgtcatga cccaggacga tcttcctttc gcgaaaagtg ccgcgctggt agtgaagaat
    43141 ccctacctga cttacgcgcg catggcgcaa attttagata ccacgccgca gcccgcgcag
    43201 aacattgcac ccagtgcggt gatcgacgcg acggcgaagc tgggtaacaa cgtatcgatt
    43261 ggcgctaacg cggtgattga gtccggcgtt gaactgggcg ataacgtgat tatcggtgcc
    43321 ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt cgcgtctctg ggcgaacgta
    43381 accatttacc atgagatcca gatcggtcag aattgcctga tccagtccgg aacagtggta
    43441 ggcgcagacg gctttggtta tgccaacgat cgtggtaact gggtgaagat cccacagatt
    43501 ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct gcacaaccat cgatcgcggc
    43561 gcgctggatg acactgttat tggcaatggc gtgatcattg ataaccagtg ccagattgca
    43621 cataacgtcg tgattggcga caatacggcg gttgccggtg gcgtcattat ggcgggcagc
    43681 ctgaaaattg gtcgttactg catgatcggc ggagccagcg taatcaacgg gcatatggaa
    43741 atatgcgaca aagtgacggt tacgggcatg ggtatggtga tgcgtcccat cactgaacca
    43801 ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag tctggcgcaa aaccgctgca
    43861 ctggtgatga acattgatga catgagcaag cgtctgaaat cgcttgagcg caaggttaat
    43921 caacaagact aacgttccat cttttgttcg ccaaacttta cggcctgtct cattcttacg
    43981 attgcggcag gccgtgttat tattgtcgtt tcttatattt tgacaggaag agtatcttga
    44041 ctactaacac tcatactctg cagattgaag agattttaga acttctgccg caccgtttcc
    44101 cgttcttact ggtggatcgc gtgctggatt ttgaagaagg tcgttttctg cgcgcagtaa
    44161 aaaatgtctc tgtcaatgag ccattcttcc agggccattt ccctggaaaa ccgattttcc
    44221 cgggtgtgct gattctggaa gcaatggcac aggcaacagg tattctggcg tttaaaagcg
    44281 taggaaaact ggaaccgggt gagctgtact acttcgctgg tattgacgaa gcgcgcttca
    44341 agcgcccggt cgtgcctggc gatcaaatga tcatggaagt cactttcgaa aaaacgcgcc
    44401 gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg taaagtagtt tgcgaagcaa
    44461 cgatgatgtg tgctcgtagc cgggaggcct gataagtgat tgataaatcc gcctttgtgc
    44521 atccaaccgc cattgtggaa gagggcgcgt caattggcgc gaacgcacac attggtcctt
    44581 tttgtatcgt tggaccccat gtcgaaattg gtgagggtac cgtactgaaa tctcacgttg
    44641 tcgtgaatgg tcatactaaa attggccgcg ataatgagat ttatcagttc gcctccatcg
    44701 gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac ccgtgtggaa atcggcgatc
    44761 gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac agtccagggc ggtggattga
    44821 cgaaggtggg cagcgacaac ttactgatga tcaacgcgca cattgcgcac gattgtacgg
    44881 taggtaaccg ctgtattctc gccaacaacg caacgctggc gggtcacgta tcggttgacg
    44941 acttcgcgat catcggcggc atgaccgcag tccatcagtt ctgcatcatt ggtgcgcacg
    45001 tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc tccttatgtc attgcgcagg
    45061 gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct gaagcgccgc ggattcagcc
    45121 gtgaggcgat taccgctatc cgcaatgcgt ataagctgat ttatcgtagc ggtaaaacgc
    45181 tcgatgaagt gaaaccggaa attgctgaac tggcggaaac atatccggaa gtgaaagcct
    45241 ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg ttaatgactg aacagcgtcc
    45301 attaacgatt gccctggtcg ccggagaaac ctccggcgat atcctggggg ccggtttaat
    45361 ccgcgctctg aaagaacgtg tgcccaacgc ccgctttgtt ggtgttgccg ggccacgaat
    45421 gcaggctgaa ggctgcgaag cctggtacga aatggaagaa ctggcggtga tgggcattgt
    45481 tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt gccgatctga caaagcgttt
    45541 tggcgaactg aagccagata tttttgttgg tattgatgcg cctgacttca atattactct
    45601 tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat tacgtcagtc catccgtctg
    45661 ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc accgatctgg tgctcgcatt
    45721 tctgcctttc gaaaaagcgt tttatgacaa atacaatgta ccgtgccgct ttatcggtca
    45781 taccatggct gatgccatgc cgttagatcc agataaaaat gccgcccgtg atgtgctggg
    45841 gatccctcac gatgcccact gcctggcgtt gctaccaggg agccgtggtg cagaagttga
    45901 aatgcttagt gccgatttcc tgaaaacggc ccagcttttg cgccagacat atccggatct
    45961 cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag cagtttgaac gcatcaaagc
    46021 tgaagtcgcg ccagaccttt cagttcattt gctggatggg atgggccgtg aggcgatggt
    46081 cgccagcgat gcggcgctac tggcgtcggg tacggcagcc ctggagtgta tgctggcgaa
    46141 atgcccgatg gtggtgggat atcgcatgaa gccttttacc ttctggttgg cgaagcggct
    46201 ggtgaaaact gattatgtct cgctgccaaa tctgctggcg ggtagagagt tagtcaaaga
    46261 attattgcag gaagagtgtg agccgcaaaa actggctgcg gcgctgttac cgctgttggc
    46321 gaacgggaaa accagccacg cgatgcacga taccttccgt gaactgcatc agcagatccg
    46381 ctgcaatgcc gatgagcagg cggcacaagc cgttctggag ttagcacaat gatcgaattt
    46441 gtttatccgc acacgcagct ggttgcgggt gtggatgaag tcggacgcgg gccgttagtt
    46501 ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc gcccgattgc cgggctgaat
    46561 gattccaaaa agctgagcga aaaacgccgt ctggcgctct atgaagagat caaagagaaa
    46621 gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa tcgacgagct gaacattctt
    46681 catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc tgcatattgc gccggaatat
    46741 gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc ctgcgatggc tgtggtgaaa
    46801 ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc tggcgaaagt gacgcgtgac
    46861 gccgaaatgg cggcgctgga tattgttttc ccgcaatatg gttttgccca acacaaaggg
    46921 tacccaaccg cttttcatct ggaaaaactg gctgaacacg gcgcgaccga acaccatcgg
    46981 cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt cctgattctt gtgtcgagat
    47041 taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg tacacctgcg ggtgcacagc
    47101 gactactcga tgatcgatgg cctggccaaa accgcaccgt tggtaaaaaa ggcggcggcg
    47161 ttgggtatgc cagcactggc gatcaccgat ttcaccaacc tttgtggtct ggtgaagttc
    47221 tacggagcgg gacatggcgc agggattaag cctatcgtcg gggcagattt taacgtccag
    47281 tgcgacctgc tgggtgatga gttaacccac ctgacggtac tggcggcgaa caataccggc
    47341 tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc gcgggtacgg tgccgccggg
    47401 ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag ggttgatcct tctttccggc
    47461 ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta acagcgcgct ggtagatgag
    47521 tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt ttctcgagct gatccgcacc
    47581 ggcaggccgg atgaagaaag ctatctgcac gcggcggtgg aactggcgga agcgcgcggt
    47641 ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca gcagcgactt tgacgcacac
    47701 gaaatccgcg tcgcgatcca cgacggcttt accctcgacg atcctaaacg cccgcgtaac
    47761 tattcgccgc agcaatatat gcgtagcgaa gaggagatgt gtgagctgtt tgccgacatc
    47821 cccgaagccc ttgccaacac cgttgagatc gccaaacgct gtaacgtaac cgtgcgtctt
    47881 ggtgaatact tcctgccgca gttcccgacc ggggacatga gcaccgaaga ttatctggtc
    47941 aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt tattccctga cgaggaagaa
    48001 cgtgttaagc gccgcccgga atatgacgaa cgtctggaga ctgaacttca ggttatcaac
    48061 cagatgggct tcccgggcta cttcctcatc gttatggaat ttatccagtg gtcgaaagat
    48121 aacggcgtac cggtagggcc aggccgtggc tccggtgcgg gttcactggt ggcctacgcg
    48181 ctgaaaatca ccgacctcga tccgctggaa tttgacctgc tgttcgaacg tttccttaac
    48241 ccggaacgtg tctccatgcc tgacttcgac gttgacttct gtatggagaa acgcgatcag
    48301 gttattgagc acgtagcgga catgtacggt cgtgatgcgg tatcgcagat catcaccttc
    48361 ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc gcgtgctggg gcatccgtac
    48421 ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc cggggatgac gctggcgaaa
    48481 gcgtttgaag ccgagccgca gctgccggaa atctacgaag cggatgaaga agttaaggcg
    48541 ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta acgccggtaa gcacgccggt
    48601 ggggtggtta tcgcgccgac caaaattacc gattttgcgc cgctttactg cgatgaagag
    48661 ggcaaacatc cggtcaccca gtttgataaa agcgacgttg aatacgccgg actggtgaag
    48721 ttcgacttcc ttggtttgcg tacgctcacc atcatcaact gggcgctgga gatgatcaac
    48781 aagcggcggg cgaagaatgg cgagccgccg ctggatatcg ccgcgatccc gctggatgac
    48841 aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg cggtattcca gcttgaatcg
    48901 cgcggcatga aggacctgat caagcgtctg caacctgact gcttcgaaga tatgatcgca
    48961 ctggtggcgc tgttccgccc aggtccgttg caatcaggga tggtggataa ctttatcgac
    49021 cgtaaacatg gtcgcgaaga aatctcctat ccggacgtac agtggcagca tgaaagcctg
    49081 aaaccggtac tggagccaac ctacggcatc atcctgtatc aggaacaggt catgcagatt
    49141 gcgcaggtgc tttctggtta taccctcggt ggcgcggata tgctgcgtcg tgcgatgggt
    49201 aagaaaaagc cggaagagat ggctaagcag cgttctgtat ttgctgaagg tgcagaaaag
    49261 aacggaatca acgccgaact ggcgatgaaa atcttcgacc tggtggagaa attcgcgggt
    49321 tacggattta acaaatcgca ctctgcggcc tatgctttgg tgtcatatca aacgttatgg
    49381 ctgaaagcgc actatcctgc ggagtttatg gcggcggtaa tgaccgccga tatggacaac
    49441 accgagaagg tggtgggcct ggtggatgag tgctggcgga tggggctgaa aatcctgcca
    49501 ccagatataa actccggtct ttaccatttc cacgtcaacg acgacggcga aatcgtgtat
    49561 ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg aggccatcat cgaagcccgt
    49621 aataaaggcg gctacttccg cgaactgttt gatctttgcg cccgtaccga caccaaaaag
    49681 ttaaaccggc gggtgctgga aaaactgatc atgtccgggg cgtttgaccg tcttggacca
    49741 caccgcgcgg cactgatgaa ctcgctgggc gatgcgttaa aagcggcaga tcaacacgcg
    49801 aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc tggccgaaga gccggaacaa
    49861 attgaacaat cctacgccag ctgccaaccg tggccggagc aggtggtatt agatggggaa
    49921 cgtgaaacgt taggcctgta cctgaccgga caccctatca accagtattt aaaagagatt
    49981 gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc cgacagaacg tggtaaagtc
    50041 atcacggctg cggggctcgt tgttgccgcg cgggttatgg tcaccaagcg cggcaatcgt
    50101 atcggtatct gcacgctgga tgaccgttcc gggcggctgg aagtgatgtt gtttactgac
    50161 gccctggata aataccagca attgctggaa aaagaccgca tacttatcgt cagcggacag
    50221 gtcagctttg atgacttcag cggtgggctt aaaatgaccg ctcgcgaagt gatggatatt
    50281 gacgaagccc gggaaaaata tgctcgcggg cttgctatct cgctgacgga caggcaaatt
    50341 gatgaccagc ttttaaaccg actccgtcag tctctggaac cccaccgctc tgggacaatt
    50401 ccagtacatc tctactatca gagggcggat gcacgcgcgc ggttgcgttt tggcgcgacg
    50461 tggcgtgtct ctccgagcga tcgtttatta aacgatctcc gtggcctcat tggttcggag
    50521 caggtggaac tggagtttga ctaatacagg aatactatga gtctgaattt ccttgatttt
    50581 gaacagccga ttgcagagct ggaagcgaaa atcgattctc tgactgcggt tagccgtcag
    50641 gatgagaaac tggatattaa catcgatgaa gaagtgcatc gtctgcgtga aaaaagcgta
    50701 gaactgacac gtaaaatctt cgccgatctc ggtgcatggc agattgcgca actggcacgc
    50761 catccacagc gtccttatac cctggattac gttcgcctgg catttgatga atttgacgaa
    50821 ctggctggcg accgcgcgta tgcagacgat aaagctatcg tcggtggtat cgcccgtctt
    50881 gacggtcgtc cggtgatgat cattggtcat caaaaaggtc gtgaaaccaa agaaaaaatt
    50941 cgccgtaact ttggtatgcc agcgccagaa ggttaccgca aagcactgcg tctgatgcaa
    51001 atggctgaac gctttaagat gcctatcatc acctttatcg acaccccggg ggcttatcct
    51061 ggcgtgggcg cagaagagcg tggtcagtct gaagccattg cacgcaacct gcgtgaaatg
    51121 tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg aaggtggttc tggcggtgcg
    51181 ctggcgattg gcgtgggcga taaagtgaat atgctgcaat acagcaccta ttccgttatc
    51241 tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg acaaagcgcc gctggcggct
    51301 gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga aactgatcga ctccatcatc
    51361 ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga tggcggcatc gttgaaagcg
    51421 caactgctgg cggatctggc cgatctcgat gtgttaagca ctgaagattt aaaaaatcgt
    51481 cgttatcagc gcctgatgag ctacggttac gcgtaattcg caaaagttct gaaaaagggt
    51541 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
    51601 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
    51661 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
    51721 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgcggcgtga
    51781 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
    51841 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
    51901 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
    51961 gtatgcgtca gtacaccgac gaatatcttg ataacattac accgccgttc acgaaagcct
    52021 tgtttaccta cgttaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
    52081 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
    52141 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
    52201 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
    52261 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
    52321 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
    52381 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
    52441 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
    52501 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
    52561 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
    52621 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
    52681 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
    52741 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
    52801 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagacggcg gcggcgatgc
    52861 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
    52921 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
    52981 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
    53041 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
    53101 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcagcg ctggtggcaa
    53161 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
    53221 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
    53281 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
    53341 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
    53401 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
    53461 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
    53521 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
    53581 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
    53641 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
    53701 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
    53761 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
    53821 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
    53881 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
    53941 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
    54001 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
    54061 tgatgacatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
    54121 ccgtgtcgat ccatacacgc aaaaacgctt caccttcttt aacgatccgg acgggctgcc
    54181 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
    54241 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
    54301 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
    54361 tggtgcagtg gcggacggaa aatccgggcg tcactctgcg cgctattcat gtgcatcacg
    54421 gtttaagtgc caatgccgat gcctgggtta cgcattgcga aaacgtctgc caacagtggc
    54481 aggtgccgct ggtggtcgaa cgcgtacaac ttgcgcaaga ggggctgggg attgaggccc
    54541 aagcgcggca ggcgcgttat caggcatttg cccgtaccct attgcccggc gaagtgctgg
    54601 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
    54661 gcggccctgc cgggctttcg gctatggggg aagtctcgga gtttgccgga acacggctta
    54721 tccgcccgtt gcttgcccgc acgcgggggg aactggcgca gtgggcattg gcgcatggtt
    54781 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
    54841 tgcgcgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
    54901 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
    54961 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctagcg atgagtgatg
    55021 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
    55081 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
    55141 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaact
    55201 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
    55261 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
    55321 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
    55381 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
    55441 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
    55501 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
    55561 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggac
    55621 tcgctcacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgt
    55681 agctcacggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
    55741 aaatcgctgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
    55801 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgta
    55861 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
    55921 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
    55981 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
    56041 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
    56101 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
    56161 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
    56221 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
    56281 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
    56341 cgtatgatgc tgcgcttgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
    56401 cgtggtttgt gtgccgatga tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
    56461 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
    56521 gccgcagaag tggtgctgtt tacctataat agtcgggcgg cgcaaatctg gtggcagcaa
    56581 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
    56641 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
    56701 ggcgtgatct ggttatcgga cgataagaat aatctggaag ttaacttaac cgcctggcaa
    56761 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
    56821 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggccattc
    56881 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctactcg
    56941 ccgccagcca tcatttgatc agcagtgatg gcgtgattgt cattaaggca caggaatacc
    57001 gcagtcagga actgaaccgc gaagccgcgc tagcccggct ggtggcagtg attaaagatt
    57061 taacaacaga acaaaaagcc cgacgaccca cgcggcccac ccgtgcatcg aaagagcgca
    57121 ggctggcatc gaaagcacaa aaatcaagcg tgaaggcgat gcgcggcaaa gtacgcagcg
    57181 gtcgggaata aaaagaagga atagatggtg aaaaaagcga tagtgacagc gatggctgta
    57241 atcagcctct ttactctgat gggatgtaat aatcgggccg aagttgatgc gctttctccg
    57301 gcgcaggctg ccgaactgaa accgatgccg caaagttggc gcggcgtgct gccgtgtgct
    57361 gattgcgaag gaatcgaaac ctctctgttc ctcgaaaaag acggaacatg ggtgatgaat
    57421 gagcgttatc tcggtgctcg tgaagaacct tcctccttcg cttcctacgg tacatgggcg
    57481 cgaaccgctg acaagctggt attaaccgac agcaaaggtg aaaagtcata ttatcgggcg
    57541 aaaggcgatg cgctggagat gctcgatcgt gaaggcaatc cgattgaatc gcagttcaac
    57601 tatacgctgg aaccggcaca atccagttta cctatgacgc cgatgaccct gcggggcatg
    57661 tatttttata tggctgatgc ggcgaccttc actgattgcg cgaccggaaa acgtttcatg
    57721 gtagcgaata acgcagagct ggagcgtggc tacctggctg cgcgcggcca cagtgaaaaa
    57781 ccgatgttac tgtcagtaga aggtcacttt acgcttgaag ctaatccgga taccggtgcg
    57841 ccgactaaag tattggcacc cgatacggca gggaaatttt acccaaacca ggattgcagt
    57901 agtttggggc agtaaacctg cttaaggcag aaacaaacgc aaaattgcct gatgtgctgc
    57961 gcttatcagg cctacagcat ataatgcaag attttgaatt agcacgattt tgtaggccgg
    58021 ataaggcgtt tacgccgcat ccggcatttt gtgcacgagg ctggctttat cactgagtca
    58081 gcccaacaaa gcgacccgcc ttgatataaa tcccaggctt caggtgccca acatactgta
    58141 actgcgtttc tggcttaaac gcagagacat caccaatacg catatgcatc agatcggcag
    58201 ggctaatcca gcccgattgc gccagtgtaa gtggatgccc ggctttggca aaggcatccg
    58261 tgacaaattc cgaacaaaac cacgactttt tgtctccttc gcccacactg cttaactgcg
    58321 ctttcgccag gccgctgacg cactttggcg aaaatcctcg gagaacggat tcagtgagca
    58381 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataaccgct
    58441 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
    58501 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacaatctg
    58561 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agaaagattg caacgtgact
    58621 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
    58681 cagcaaatcg ccgggtttga gatcaggtgc cgttatttct tttattgatt gttcggtgaa
    58741 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
    58801 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
    58861 aaacaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaaagggc
    58921 cctgaaattc aggacccttt ctggcatcag cctttaatct gtttcaccag atattcgacg
    58981 atgtcaccag tcttaattaa ctgtttctcg ccgttgcgac gatatttata ttcgatatcg
    59041 tcgttgtcga ggttacggtc gcccagcaca atagtgtgcg gaataccgat cagttccata
    59101 tcagcaaaca tcacgcccgg acgctctttg cggtcatcca gcagcacttc gataccttgt
    59161 gcacgcagtt cgctgtacag tttctcagca agctcttgta cgcggaagga tttgtgcatg
    59221 ttcatcggca gaatcgccac ctggaacggc gcgatagcgt caggccatac gatgcctcgt
    59281 tcgtcgtagt tctgctcaat cgccgcagct accacacgcg ttaccccgat accgtagcaa
    59341 cccatcgtca ggatttggtt acggccatct tcaccctgta cggaggcttt cagtgcttcg
    59401 gagtacttgg tacccagctg gaagatgtga ccaacttcga taccacgttt gatcagcagc
    59461 gtaccctggc catccgggct tggatcgcca gccaccacgt tacggatatc tgcaacttcc
    59521 ggggtagcga catcgcgatc ccagttgatg ccgaagtagt gtttaccatc gatgttagca
    59581 ccagcagcga aatcactcat cgccgcaacg gtacggtcaa tcaccaccgg aatcggcatg
    59641 tttaccggac ccagtgaacc cggaccggct ttaaccacgg cacgaatttc ttcttcggtc
    59701 gcgaaagtca gcgggcttgc aacctgcggc agtttttctg ctttaacttc gttcagctcg
    59761 tgatcaccgc gcaccagcag cgcaaccagc gggaagctac tgccttcaac cgctttaacc
    59821 agcagagtct taaccgtttt ctcaatcggc agattgaact gttcaaccag ttccgcgatg
    59881 gttttcgcgt tcggcgtatc aaccagcgtc atttcctggg tagcagcagc gcgcggttct
    59941 ttcggcgcga tagcttctgc cagttcaatg ttcgctgcat agtcagaggt gtcggagaag
    60001 accacatcgt cttcaccgct ctgcgccagc acctggaatt cgtgagaggc gctgccgccg
    60061 atagaaccgg tgtcggcttg tacggcgcgg aaatccagcc ccatgcggct gaagattttg
    60121 ctgtaggccg catacattgc atcgtaggtt tcctgcaggg attcctgaga agtatggaaa
    60181 gagtaagcat ctttcatcag gaattcgcgg gaacgcatga cgccgaaacg cggacgcact
    60241 tcgtcgcgga acttggtctg gatctgatag aagttcagcg gcagctgttt gtaagagcta
    60301 agctcgttac gaatcaggtc agtgataact tcttcatgag ttgggccgag tacgaacgga
    60361 cgctcgccac ggtcaacaaa acgcagcagt tccggaccgt actgttccca acgaccactc
    60421 tcttgccaca aatcagctgg ctgaaccacc ggcatcgaca cctcgatcgc accggcgttg
    60481 ttcatctctt cacgcacgat gttttcgact tttttcagaa cgcgcacgcc ggtcggcagc
    60541 caggtatata acccggaggc cagcttgcgg atcatcccgg cgcgcagcat cagctgatgg
    60601 ctgatcacct cggcgtcggc aggtgtctcc ttgagagtgg agagcaggta ttggctagta
    60661 cgcatgttgt tacggttcca gttggaaggt agaacaggct caaggcgagc ctgggacaaa
    60721 aaaaagtgat ttagtttacc agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa
    60781 cgcggttcca gcgcaaagac ttcaaaacct acgtcggtga cgcgccagcg aacgttaaaa
    60841 tcatgtagcc agacggcata ggttttgccc gtttcctcac ctttacgata ggccgggcgt
    60901 gggtcctgcg ccagtacttc gcggataaac agcgttaact gcggataacg cttctccagc
    60961 gtcaaaagct gcttttcgac ctctgcggta aaactcaccg ccatctctgc cgctggcgca
    61021 ctttgcgcat agctggcact ggcatcggga agcgattcgg caaaggggag atacggtttg
    61081 atatccacta ccggcgtacc atcgaccaga tccagactgc cgagcttcag aatcacgctg
    61141 tctttatggc aaacaacctc tttcagctct accagcgaca taccaattgg gttagggcgg
    61201 aaagtagagc gtgttgcgaa aacccccatt ctggcgttac cgccgaggcg cggtggacgc
    61261 acagtcggac gccagccgcc ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg
    61321 ctgaacgctt ccaggccgcg aacggcgtcg gcctggttgt agggagcaat gagatgcagt
    61381 tcgccgttgg cgctttttac cagacctggc tggcgcggaa cggcgaattt ttctttatag
    61441 ggcgagcgaa taacgcctat ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa
    61501 gcgcagaacc gatacataca gcctgacgat agcagcctgg cgtaccgctg gtgacttcgc
    61561 agctatgcag taataccgca ttggccttca ttttcgaggc gttgatttgc atccgcttac
    61621 gtgcggttgg aatgctcggc ggagagtcct gattggaggc ctggcaagaa tcgccactga
    61681 cttcaccgag atcgcggaac ggtttgccga ctaattcttc tgcattggta taaattcgga
    61741 ccggcgtggc gcgcggcgct ttcggttttg caggctccgc tttcggctgg ggtgcagtgc
    61801 tttgaacggg ttcgacaggg gatctgctta acatggaaca gccgcttagc atgagtgcta
    61861 ctaaacagat cggtaaagca cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat
    61921 tgaatcagga gcttgtaaaa atgacaagac gggcaagcgc ccgtcctgaa tgatattaca
    61981 aattgtggaa acagcctaaa aattaccagc ctttaacagc gccgccgtta aacactttgt
    62041 ttgctgcttc gtaaacttca tcagactgat aagcctggac gaatttcttc acgttctcgg
    62101 cgtctttgtt atcttcacgc gttacgatca ggtttacgta cggggagtct ttatcttcga
    62161 caaagatacc gtctttcgct ggagtcaggc caatctggct ggcataggtg gtattgataa
    62221 ctgccagagc gatttgcgcg tcgtccagag atcgaggcag ctgcggtgct tccagctcaa
    62281 caattttcag attttttggg ttctcaacaa catcaagaac ggtcggcagc aggccaacgc
    62341 catctttcag tttgatcaag cccacttttt gcagcagcag cagtgaacga ccaagattag
    62401 ttggatcgtt tggcacggca acctgcgaac catcctgcag ttcatccagt gatttgattt
    62461 tcttggagta accagcaatc ggataaacaa aagtgttgcc tactgcgacc agtttgtagc
    62521 cacgatcttt cagttgctga tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat
    62581 cgcctttgct caatgcttcg tttggcagaa catagtcgtt gaaggttacc agctcaacgt
    62641 ccaggccata tttgtctttc gcaactttct gcgcgacttc tgctacctgc tgttcggcac
    62701 caacaatcac gccgacttta atgtggtttg gatctttttc atcctgaccg cagcctacca
    62761 gtgccagtga tccaatcagg gctcccactg ccgcaaaggt tttgaatttg aacgccatac
    62821 cttattcctt cttcaattat ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga
    62881 cgatgcggtc gcctgcgaac tgaattaaat aaaccagaat gaccagcaat accagtaccg
    62941 tattcatcac cgtcgcgtta tagccgatgt agccatactg atagccaatc tgacctaaac
    63001 caccggcacc gactgcacca cccatcgcgg aatagccgac cagggtaatc agggtgatag
    63061 ttgccgcatt caccagaccc ggcagtgctt ccggtaacag caccttacgg acgatctgca
    63121 tcggcgtggc acccattgcg cgggaagctt caattaaccc ggttgggatc tccagcagag
    63181 cgttctcgac catacgggca ataaacggtg ctgcaccaac ggttaacgga acaatcgctg
    63241 cctgcaaacc aatcgatgta ccgacaataa cgcgggtaaa cggaatcatc cataccagca
    63301 agataatgaa cgggatggaa cggaaaatgt tcacaatcgc agaaacggta cgatacagct
    63361 tcgcgttagc aataatttgc cccgggcgcg taacataaag caggacgccg accggcagac
    63421 caatcacaaa gccaaaaaat ccggatacga aggtcattgc cagcgtttcc catacgccac
    63481 gaaccagcaa ccacatcatc ggctcagaca taacccagta cctctacttt tacatggtgt
    63541 tcctgcagcc aggcaatggc ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc
    63601 atgatgccga acttaacgcc accggcgtaa tccatctgcg cgctaataat gttgttgttg
    63661 acgttgaaac gacgcgcggt ttcagaaagc agcggggcat cgaccgattg accggtaaac
    63721 tccaggcgca acatcggcac gcagtcagta aatggctccg cttgcagacg ttcctggtaa
    63781 tcttccggga tatccagatg cagggtcgac tgaataaact tctgcgccag cggcgttttc
    63841 ggatgcgaga acacttcact taccgtgtcc tgctcgatca gttcgccatt gctgatgacc
    63901 gccacgcaat cacaaatacg cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc
    63961 aaacccagac ggcggttaat gtctttcagc agttcgagaa tagaacgtgt cgttgccggg
    64021 tccagcgcgc tggtggcttc atcacacagc aatactttgg gattgctggc taatgcacgg
    64081 gcaatcgcca cacgttgttt ctgcccaccg gaaagattcg acgggtagct atcatgctta
    64141 tcgccaagac caaccaatga cagcaattcc gtcacgcgac gtttgatctc gtctttcggt
    64201 gtgttgtcca gctccagcgg cagagccacg ttgccaaaaa cagtacgcga agagagcagg
    64261 ttaaaatgct ggaaaatcat accaatctgg cggcgagctt tggtcaactc ggattctgac
    64321 agcgtggtca gttcctggcc atcgaccagc acgctaccct cggttgggcg ctccagcagg
    64381 tttacacaac gtataagcgt actcttaccc gcgcctgagg caccgataac gccataaatt
    64441 tgtccagctg gcacatgcag gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg
    64501 tggaacactt tggtgatatt cgaaagtttt atcattgatt atttattatc gtcattaagt
    64561 tagtcgtggc atctcgaatg cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg
    64621 catccagacg tctaaatcaa tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca
    64681 tgcgatacta gcgtcccatg ccttattaag gagctataaa aggtggcgaa gagcgtaccc
    64741 gcaatttttc ttgaccgtga tggcaccatt aatgtcgatc acggctatgt ccatgagatc
    64801 gacaactttg aatttatcga cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc
    64861 tttgcgctgg tggtagtaac caaccagtct ggcattgctc gcggtaaatt taccgaagca
    64921 cagtttgaaa cgctgaccga gtggatggac tggtcgctgg cggaccgaga tgtcgatctg
    64981 gatggtatct attattgccc gcatcatccg cagggtagtg ttgaagagtt tcgccaggtc
    65041 tgcgattgcc gcaaaccaca tccggggatg cttttgtcag cacgcgatta tttgcatatt
    65101 gatatggccg cttcttatat ggtgggcgat aaattagaag atatgcaggc agcggttgcg
    65161 gcgaacgtgg gaacaaaagt gctggtgcgt acgggtaaac ctattacacc tgaagcagaa
    65221 aacgcggcag attgggtgtt aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag
    65281 caaaaaccgg cgcaatgatt aaaagatgag cggttgaaat aaaaatgcat ttttccgctt
    65341 gtcttcctga gccgactccc tataatgcgc ctccatcgac acggcgaatg tgaatcactt
    65401 cacacaaaca gccggttcgg ttgaagagaa aaaatcctga aattcagggt tgactctgaa
    65461 agaggaatgc gtaatatacg ccacctcgcg acagtgagct gaaagc
//
LOCUS       DAZGAN020000014        61266 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGAN020000014 DAZGAN020000000
VERSION     DAZGAN020000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 61266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.014"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(52..1416)
                     /gene="rhlE"
                     /locus_tag="ACHKYO_RS05180"
                     /old_locus_tag="pgaptmp_001036"
     CDS             complement(52..1416)
                     /gene="rhlE"
                     /locus_tag="ACHKYO_RS05180"
                     /old_locus_tag="pgaptmp_001036"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HGU4427925.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            1645..2316
                     /gene="cecR"
                     /locus_tag="ACHKYO_RS05185"
                     /old_locus_tag="pgaptmp_001037"
     CDS             1645..2316
                     /gene="cecR"
                     /locus_tag="ACHKYO_RS05185"
                     /old_locus_tag="pgaptmp_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HGU4427926.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2319..3314
                     /gene="hlyD"
                     /locus_tag="ACHKYO_RS05190"
                     /old_locus_tag="pgaptmp_001038"
     CDS             2319..3314
                     /gene="hlyD"
                     /locus_tag="ACHKYO_RS05190"
                     /old_locus_tag="pgaptmp_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HGU4427927.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            3307..5043
                     /gene="ybhF"
                     /locus_tag="ACHKYO_RS05195"
                     /old_locus_tag="pgaptmp_001039"
     CDS             3307..5043
                     /gene="ybhF"
                     /locus_tag="ACHKYO_RS05195"
                     /old_locus_tag="pgaptmp_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU4427928.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHAEGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKAHQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5036..6169
                     /gene="ybhS"
                     /locus_tag="ACHKYO_RS05200"
                     /old_locus_tag="pgaptmp_001040"
     CDS             5036..6169
                     /gene="ybhS"
                     /locus_tag="ACHKYO_RS05200"
                     /old_locus_tag="pgaptmp_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4427929.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6180..7286
                     /gene="ybhR"
                     /locus_tag="ACHKYO_RS05205"
                     /old_locus_tag="pgaptmp_001041"
     CDS             6180..7286
                     /gene="ybhR"
                     /locus_tag="ACHKYO_RS05205"
                     /old_locus_tag="pgaptmp_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4427930.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7248..7658)
                     /gene="ybhQ"
                     /locus_tag="ACHKYO_RS05210"
                     /old_locus_tag="pgaptmp_001042"
     CDS             complement(7248..7658)
                     /gene="ybhQ"
                     /locus_tag="ACHKYO_RS05210"
                     /old_locus_tag="pgaptmp_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HGU4427931.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7791..8552
                     /gene="ybhP"
                     /locus_tag="ACHKYO_RS05215"
                     /old_locus_tag="pgaptmp_001043"
     CDS             7791..8552
                     /gene="ybhP"
                     /locus_tag="ACHKYO_RS05215"
                     /old_locus_tag="pgaptmp_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU4427932.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRVHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8549..9790
                     /gene="clsB"
                     /locus_tag="ACHKYO_RS05220"
                     /old_locus_tag="pgaptmp_001044"
     CDS             8549..9790
                     /gene="clsB"
                     /locus_tag="ACHKYO_RS05220"
                     /old_locus_tag="pgaptmp_001044"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HGU4427933.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9790..10746
                     /gene="ybhN"
                     /locus_tag="ACHKYO_RS05225"
                     /old_locus_tag="pgaptmp_001045"
     CDS             9790..10746
                     /gene="ybhN"
                     /locus_tag="ACHKYO_RS05225"
                     /old_locus_tag="pgaptmp_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HGU4427934.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10782..11495)
                     /gene="ybhM"
                     /locus_tag="ACHKYO_RS05230"
                     /old_locus_tag="pgaptmp_001046"
     CDS             complement(10782..11495)
                     /gene="ybhM"
                     /locus_tag="ACHKYO_RS05230"
                     /old_locus_tag="pgaptmp_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU4427935.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11700..12404)
                     /gene="ybhL"
                     /locus_tag="ACHKYO_RS05235"
                     /old_locus_tag="pgaptmp_001047"
     CDS             complement(11700..12404)
                     /gene="ybhL"
                     /locus_tag="ACHKYO_RS05235"
                     /old_locus_tag="pgaptmp_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU4427936.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12541..12993)
                     /gene="moaE"
                     /locus_tag="ACHKYO_RS05240"
                     /old_locus_tag="pgaptmp_001048"
     CDS             complement(12541..12993)
                     /gene="moaE"
                     /locus_tag="ACHKYO_RS05240"
                     /old_locus_tag="pgaptmp_001048"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HGU4427937.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(12995..13240)
                     /gene="moaD"
                     /locus_tag="ACHKYO_RS05245"
                     /old_locus_tag="pgaptmp_001049"
     CDS             complement(12995..13240)
                     /gene="moaD"
                     /locus_tag="ACHKYO_RS05245"
                     /old_locus_tag="pgaptmp_001049"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HGU4427938.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            complement(13233..13718)
                     /gene="moaC"
                     /locus_tag="ACHKYO_RS05250"
                     /old_locus_tag="pgaptmp_001050"
     CDS             complement(13233..13718)
                     /gene="moaC"
                     /locus_tag="ACHKYO_RS05250"
                     /old_locus_tag="pgaptmp_001050"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HGU4427939.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(13721..14233)
                     /gene="moaB"
                     /locus_tag="ACHKYO_RS05255"
                     /old_locus_tag="pgaptmp_001051"
     CDS             complement(13721..14233)
                     /gene="moaB"
                     /locus_tag="ACHKYO_RS05255"
                     /old_locus_tag="pgaptmp_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HGU4427940.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14255..15244)
                     /gene="moaA"
                     /locus_tag="ACHKYO_RS05260"
                     /old_locus_tag="pgaptmp_001052"
     CDS             complement(14255..15244)
                     /gene="moaA"
                     /locus_tag="ACHKYO_RS05260"
                     /old_locus_tag="pgaptmp_001052"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HGU4427941.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15232..15376)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15641..16549
                     /gene="yvcK"
                     /locus_tag="ACHKYO_RS05265"
                     /old_locus_tag="pgaptmp_001053"
     CDS             15641..16549
                     /gene="yvcK"
                     /locus_tag="ACHKYO_RS05265"
                     /old_locus_tag="pgaptmp_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HGU4427942.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(16741..18762)
                     /gene="uvrB"
                     /locus_tag="ACHKYO_RS05270"
                     /old_locus_tag="pgaptmp_001054"
     CDS             complement(16741..18762)
                     /gene="uvrB"
                     /locus_tag="ACHKYO_RS05270"
                     /old_locus_tag="pgaptmp_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGU4427943.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(19341..20018)
                     /gene="bioD"
                     /locus_tag="ACHKYO_RS05275"
                     /old_locus_tag="pgaptmp_001055"
     CDS             complement(19341..20018)
                     /gene="bioD"
                     /locus_tag="ACHKYO_RS05275"
                     /old_locus_tag="pgaptmp_001055"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU4427944.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(20011..20766)
                     /gene="bioC"
                     /locus_tag="ACHKYO_RS05280"
                     /old_locus_tag="pgaptmp_001056"
     CDS             complement(20011..20766)
                     /gene="bioC"
                     /locus_tag="ACHKYO_RS05280"
                     /old_locus_tag="pgaptmp_001056"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HGU4427945.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLEQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(20753..21907)
                     /gene="bioF"
                     /locus_tag="ACHKYO_RS05285"
                     /old_locus_tag="pgaptmp_001057"
     CDS             complement(20753..21907)
                     /gene="bioF"
                     /locus_tag="ACHKYO_RS05285"
                     /old_locus_tag="pgaptmp_001057"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HGU4427946.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(21904..22944)
                     /gene="bioB"
                     /locus_tag="ACHKYO_RS05290"
                     /old_locus_tag="pgaptmp_001058"
     CDS             complement(21904..22944)
                     /gene="bioB"
                     /locus_tag="ACHKYO_RS05290"
                     /old_locus_tag="pgaptmp_001058"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HGU4427947.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFHKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23031..24320
                     /gene="bioA"
                     /locus_tag="ACHKYO_RS05295"
                     /old_locus_tag="pgaptmp_001059"
     CDS             23031..24320
                     /gene="bioA"
                     /locus_tag="ACHKYO_RS05295"
                     /old_locus_tag="pgaptmp_001059"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HGU4427948.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            24379..24855
                     /gene="ybhB"
                     /locus_tag="ACHKYO_RS05300"
                     /old_locus_tag="pgaptmp_001060"
     CDS             24379..24855
                     /gene="ybhB"
                     /locus_tag="ACHKYO_RS05300"
                     /old_locus_tag="pgaptmp_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HGU4427949.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            25007..26290
                     /gene="ybhC"
                     /locus_tag="ACHKYO_RS05305"
                     /old_locus_tag="pgaptmp_001061"
     CDS             25007..26290
                     /gene="ybhC"
                     /locus_tag="ACHKYO_RS05305"
                     /old_locus_tag="pgaptmp_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HGU4427950.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(26524..28785)
                     /gene="ybhJ"
                     /locus_tag="ACHKYO_RS05310"
                     /old_locus_tag="pgaptmp_001062"
     CDS             complement(26524..28785)
                     /gene="ybhJ"
                     /locus_tag="ACHKYO_RS05310"
                     /old_locus_tag="pgaptmp_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HGU4427951.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYKIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(28968..30401)
                     /gene="ybhI"
                     /locus_tag="ACHKYO_RS05315"
                     /old_locus_tag="pgaptmp_001063"
     CDS             complement(28968..30401)
                     /gene="ybhI"
                     /locus_tag="ACHKYO_RS05315"
                     /old_locus_tag="pgaptmp_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HGU4427952.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(30477..31520)
                     /gene="ybhH"
                     /locus_tag="ACHKYO_RS05320"
                     /old_locus_tag="pgaptmp_001064"
     CDS             complement(30477..31520)
                     /gene="ybhH"
                     /locus_tag="ACHKYO_RS05320"
                     /old_locus_tag="pgaptmp_001064"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HGU4427953.1"
                     /translation="MPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGNDLEI
                     DGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGAFAI
                     ENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVALTF
                     LNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELDADK
                     ALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRALAI
                     TGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRASVIR
                     TTRKIFSGEVYLP"
     gene            31712..32665
                     /gene="ybhD"
                     /locus_tag="ACHKYO_RS05325"
                     /old_locus_tag="pgaptmp_001065"
     CDS             31712..32665
                     /gene="ybhD"
                     /locus_tag="ACHKYO_RS05325"
                     /old_locus_tag="pgaptmp_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4427954.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(32706..33701)
                     /gene="pgl"
                     /locus_tag="ACHKYO_RS05330"
                     /old_locus_tag="pgaptmp_001066"
     CDS             complement(32706..33701)
                     /gene="pgl"
                     /locus_tag="ACHKYO_RS05330"
                     /old_locus_tag="pgaptmp_001066"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HGU4427955.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            33856..34674
                     /gene="ybhA"
                     /locus_tag="ACHKYO_RS05335"
                     /old_locus_tag="pgaptmp_001067"
     CDS             33856..34674
                     /gene="ybhA"
                     /locus_tag="ACHKYO_RS05335"
                     /old_locus_tag="pgaptmp_001067"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HGU4427956.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(34675..35733)
                     /gene="modC"
                     /locus_tag="ACHKYO_RS05340"
                     /old_locus_tag="pgaptmp_001068"
     CDS             complement(34675..35733)
                     /gene="modC"
                     /locus_tag="ACHKYO_RS05340"
                     /old_locus_tag="pgaptmp_001068"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HGU4427957.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(35736..36425)
                     /gene="modB"
                     /locus_tag="ACHKYO_RS05345"
                     /old_locus_tag="pgaptmp_001069"
     CDS             complement(35736..36425)
                     /gene="modB"
                     /locus_tag="ACHKYO_RS05345"
                     /old_locus_tag="pgaptmp_001069"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HGU4427958.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITIPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(36425..37198)
                     /gene="modA"
                     /locus_tag="ACHKYO_RS05350"
                     /old_locus_tag="pgaptmp_001070"
     CDS             complement(36425..37198)
                     /gene="modA"
                     /locus_tag="ACHKYO_RS05350"
                     /old_locus_tag="pgaptmp_001070"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4427959.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(37365..37514)
                     /gene="acrZ"
                     /locus_tag="ACHKYO_RS05355"
                     /old_locus_tag="pgaptmp_001071"
     CDS             complement(37365..37514)
                     /gene="acrZ"
                     /locus_tag="ACHKYO_RS05355"
                     /old_locus_tag="pgaptmp_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HGU4427960.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            37643..38431
                     /gene="modE"
                     /locus_tag="ACHKYO_RS05360"
                     /old_locus_tag="pgaptmp_001072"
     CDS             37643..38431
                     /gene="modE"
                     /locus_tag="ACHKYO_RS05360"
                     /old_locus_tag="pgaptmp_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HGU4427961.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            38499..39971
                     /gene="modF"
                     /locus_tag="ACHKYO_RS05365"
                     /old_locus_tag="pgaptmp_001073"
     CDS             38499..39971
                     /gene="modF"
                     /locus_tag="ACHKYO_RS05365"
                     /old_locus_tag="pgaptmp_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HGU4427962.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKSLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            40232..41248
                     /gene="galE"
                     /locus_tag="ACHKYO_RS05370"
                     /old_locus_tag="pgaptmp_001074"
     CDS             40232..41248
                     /gene="galE"
                     /locus_tag="ACHKYO_RS05370"
                     /old_locus_tag="pgaptmp_001074"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGU4427963.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            41258..42304
                     /gene="galT"
                     /locus_tag="ACHKYO_RS05375"
                     /old_locus_tag="pgaptmp_001075"
     CDS             41258..42304
                     /gene="galT"
                     /locus_tag="ACHKYO_RS05375"
                     /old_locus_tag="pgaptmp_001075"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGU4427964.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            42308..43456
                     /gene="galK"
                     /locus_tag="ACHKYO_RS05380"
                     /old_locus_tag="pgaptmp_001076"
     CDS             42308..43456
                     /gene="galK"
                     /locus_tag="ACHKYO_RS05380"
                     /old_locus_tag="pgaptmp_001076"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGU4427965.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            43450..44490
                     /gene="galM"
                     /locus_tag="ACHKYO_RS05385"
                     /old_locus_tag="pgaptmp_001077"
     CDS             43450..44490
                     /gene="galM"
                     /locus_tag="ACHKYO_RS05385"
                     /old_locus_tag="pgaptmp_001077"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HGU4427966.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            44695..45447
                     /gene="gpmA"
                     /locus_tag="ACHKYO_RS05390"
                     /old_locus_tag="pgaptmp_001078"
     CDS             44695..45447
                     /gene="gpmA"
                     /locus_tag="ACHKYO_RS05390"
                     /old_locus_tag="pgaptmp_001078"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HGU4427967.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(45605..46657)
                     /gene="aroG"
                     /locus_tag="ACHKYO_RS05395"
                     /old_locus_tag="pgaptmp_001079"
     CDS             complement(45605..46657)
                     /gene="aroG"
                     /locus_tag="ACHKYO_RS05395"
                     /old_locus_tag="pgaptmp_001079"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HGU4427968.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            46973..47353
                     /gene="ybgS"
                     /locus_tag="ACHKYO_RS05400"
                     /old_locus_tag="pgaptmp_001080"
     CDS             46973..47353
                     /gene="ybgS"
                     /locus_tag="ACHKYO_RS05400"
                     /old_locus_tag="pgaptmp_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HGU4427969.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            47467..48408
                     /gene="zitB"
                     /locus_tag="ACHKYO_RS05405"
                     /old_locus_tag="pgaptmp_001081"
     CDS             47467..48408
                     /gene="zitB"
                     /locus_tag="ACHKYO_RS05405"
                     /old_locus_tag="pgaptmp_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HGU4427970.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGENPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(48405..49124)
                     /gene="pnuC"
                     /locus_tag="ACHKYO_RS05410"
                     /old_locus_tag="pgaptmp_001082"
     CDS             complement(48405..49124)
                     /gene="pnuC"
                     /locus_tag="ACHKYO_RS05410"
                     /old_locus_tag="pgaptmp_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGU4427971.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(49162..50205)
                     /gene="nadA"
                     /locus_tag="ACHKYO_RS05415"
                     /old_locus_tag="pgaptmp_001083"
     CDS             complement(49162..50205)
                     /gene="nadA"
                     /locus_tag="ACHKYO_RS05415"
                     /old_locus_tag="pgaptmp_001083"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HGU4427972.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(50454..50579)
                     /locus_tag="ACHKYO_RS05420"
                     /old_locus_tag="pgaptmp_001084"
     ncRNA           complement(50454..50579)
                     /ncRNA_class="other"
                     /locus_tag="ACHKYO_RS05420"
                     /old_locus_tag="pgaptmp_001084"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(50610..50685)
                     /locus_tag="ACHKYO_RS05425"
                     /old_locus_tag="pgaptmp_001085"
     tRNA            complement(50610..50685)
                     /locus_tag="ACHKYO_RS05425"
                     /old_locus_tag="pgaptmp_001085"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50650..50652),aa:Lys,seq:ttt)
     gene            complement(50719..50794)
                     /locus_tag="ACHKYO_RS05430"
                     /old_locus_tag="pgaptmp_001086"
     tRNA            complement(50719..50794)
                     /locus_tag="ACHKYO_RS05430"
                     /old_locus_tag="pgaptmp_001086"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50759..50761),aa:Lys,seq:ttt)
     gene            complement(50941..51016)
                     /locus_tag="ACHKYO_RS05435"
                     /old_locus_tag="pgaptmp_001087"
     tRNA            complement(50941..51016)
                     /locus_tag="ACHKYO_RS05435"
                     /old_locus_tag="pgaptmp_001087"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50981..50983),aa:Lys,seq:ttt)
     gene            complement(51020..51095)
                     /locus_tag="ACHKYO_RS05440"
                     /old_locus_tag="pgaptmp_001088"
     tRNA            complement(51020..51095)
                     /locus_tag="ACHKYO_RS05440"
                     /old_locus_tag="pgaptmp_001088"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51060..51062),aa:Val,seq:tac)
     gene            complement(51245..51320)
                     /locus_tag="ACHKYO_RS05445"
                     /old_locus_tag="pgaptmp_001089"
     tRNA            complement(51245..51320)
                     /locus_tag="ACHKYO_RS05445"
                     /old_locus_tag="pgaptmp_001089"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51285..51287),aa:Lys,seq:ttt)
     gene            complement(51323..51398)
                     /locus_tag="ACHKYO_RS05450"
                     /old_locus_tag="pgaptmp_001090"
     tRNA            complement(51323..51398)
                     /locus_tag="ACHKYO_RS05450"
                     /old_locus_tag="pgaptmp_001090"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51363..51365),aa:Val,seq:tac)
     gene            complement(51534..51609)
                     /locus_tag="ACHKYO_RS05455"
                     /old_locus_tag="pgaptmp_001091"
     tRNA            complement(51534..51609)
                     /locus_tag="ACHKYO_RS05455"
                     /old_locus_tag="pgaptmp_001091"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51574..51576),aa:Lys,seq:ttt)
     gene            complement(51774..52565)
                     /gene="cpoB"
                     /locus_tag="ACHKYO_RS05460"
                     /old_locus_tag="pgaptmp_001092"
     CDS             complement(51774..52565)
                     /gene="cpoB"
                     /locus_tag="ACHKYO_RS05460"
                     /old_locus_tag="pgaptmp_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HGU4427973.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(52575..53096)
                     /gene="pal"
                     /locus_tag="ACHKYO_RS05465"
                     /old_locus_tag="pgaptmp_001093"
     CDS             complement(52575..53096)
                     /gene="pal"
                     /locus_tag="ACHKYO_RS05465"
                     /old_locus_tag="pgaptmp_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HGU4427974.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(53131..54423)
                     /gene="tolB"
                     /locus_tag="ACHKYO_RS05470"
                     /old_locus_tag="pgaptmp_001094"
     CDS             complement(53131..54423)
                     /gene="tolB"
                     /locus_tag="ACHKYO_RS05470"
                     /old_locus_tag="pgaptmp_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HGU4427975.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(54556..55821)
                     /gene="tolA"
                     /locus_tag="ACHKYO_RS05475"
                     /old_locus_tag="pgaptmp_001095"
     CDS             complement(54556..55821)
                     /gene="tolA"
                     /locus_tag="ACHKYO_RS05475"
                     /old_locus_tag="pgaptmp_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HGU4427976.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(55886..56314)
                     /gene="tolR"
                     /locus_tag="ACHKYO_RS05480"
                     /old_locus_tag="pgaptmp_001096"
     CDS             complement(55886..56314)
                     /gene="tolR"
                     /locus_tag="ACHKYO_RS05480"
                     /old_locus_tag="pgaptmp_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HGU4427977.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(56318..57010)
                     /gene="tolQ"
                     /locus_tag="ACHKYO_RS05485"
                     /old_locus_tag="pgaptmp_001097"
     CDS             complement(56318..57010)
                     /gene="tolQ"
                     /locus_tag="ACHKYO_RS05485"
                     /old_locus_tag="pgaptmp_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HGU4427978.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(57007..57411)
                     /gene="ybgC"
                     /locus_tag="ACHKYO_RS05490"
                     /old_locus_tag="pgaptmp_001098"
     CDS             complement(57007..57411)
                     /gene="ybgC"
                     /locus_tag="ACHKYO_RS05490"
                     /old_locus_tag="pgaptmp_001098"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU4427979.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(57561..57854)
                     /gene="ybgE"
                     /locus_tag="ACHKYO_RS05495"
                     /old_locus_tag="pgaptmp_001099"
     CDS             complement(57561..57854)
                     /gene="ybgE"
                     /locus_tag="ACHKYO_RS05495"
                     /old_locus_tag="pgaptmp_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HGU4427980.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(57854..57967)
                     /gene="cydX"
                     /locus_tag="ACHKYO_RS05500"
                     /old_locus_tag="pgaptmp_001100"
     CDS             complement(57854..57967)
                     /gene="cydX"
                     /locus_tag="ACHKYO_RS05500"
                     /old_locus_tag="pgaptmp_001100"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HGU4427981.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(57982..59121)
                     /gene="cydB"
                     /locus_tag="ACHKYO_RS05505"
                     /old_locus_tag="pgaptmp_001101"
     CDS             complement(57982..59121)
                     /gene="cydB"
                     /locus_tag="ACHKYO_RS05505"
                     /old_locus_tag="pgaptmp_001101"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU4427982.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(59137..60705)
                     /gene="cydA"
                     /locus_tag="ACHKYO_RS05510"
                     /old_locus_tag="pgaptmp_001102"
     CDS             complement(59137..60705)
                     /gene="cydA"
                     /locus_tag="ACHKYO_RS05510"
                     /old_locus_tag="pgaptmp_001102"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HGU4427983.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT        14878 a        16293 c        15436 g        14659 t
ORIGIN      
        1 gttttgcgtt tgttcatcag cctgatgccg ggcatagccc ggcataaaag attactgcgc
       61 agcggtaggt ttacgcggac ggcggcgacg ttgttgttcg cctgccggtt tggcatcgcc
      121 gaggcggcga gacggttttt ctgcaggttt cgcgcttgca gattttgcgc caccttcccc
      181 acggcgtggt tgctgttgac cgcgaccacc accttgccca cgaccgccgc cgccacgttg
      241 ctggcgaccg ttctggatcg gttcggcttt gattgacggg tccggctcat agcccggaat
      301 cgcaatgcgc gggatctctt ttttcagcag tttttcgata tcacgcagca gtttgtgttc
      361 atcaacacac accagcgaca acgcttcacc ggtagcagcc gcacgaccgg tacgcccgat
      421 acggtggaca taatcttccg gtacgtttgg cagttcatag ttgacgacgt gcggcaactc
      481 ttcgatatcc agaccgcgcg cagcgatatc ggtcgccacc agtacgcgaa tgtcacccga
      541 tttaaaatca gccagcgcac gggtacgcgc accttgcgat ttattcccgt ggatcgccgc
      601 actacggatg ccatctttat tgagctgctc agccagatgg ttagcgccgt gtttggtacg
      661 ggtaaacacc agcacctgct gccagttccc tttaccaatc atgtgcgaca gcaattcgcg
      721 tttgcgtttc ttatcgacaa agtgaacgtg ctgggtcacc tgatcagacg cggtattgcg
      781 gcgtgccact tcgatttcca gcgggttgtg caacagtttt tccgccaggg ctttaatatc
      841 gtcagagaag gtcgcagaga acagcaggtt ctggcgtttc gctggcagtt ttgttaacac
      901 gcgacggata tcgtggataa agcccatgtc gagcatgcgg tcagcttcat cgaggacgag
      961 gatttcaacc tgatccagct tcactgcatt ttgatgttcc aggtccagca aacgtcccgg
     1021 tgttgccacc agaacatcaa cgccgccgcg cagcttcatc atttgcgggt taatgctgac
     1081 accgccaaac accaccagcg aacgaatgtt caaatatttg ctgtaatcac ggacgttttc
     1141 gccaatctgc gcagccagtt cacgggtcgg ggtaagaatg agcgcacgta ccggacgacg
     1201 ccctttggcg tgcggctggc gagtgatcag gtgttgcaac agcggcagcg taaagcccgc
     1261 tgttttaccg gtaccggtct gggcgctagc catcaggtcg cggccttcca gcaccgcagg
     1321 gatcgcctgc tgctgaatag gggtgggttc acggtaaccc tgctcggcaa cggcgcgcag
     1381 gatatcaggg cttaaaccca aagaatcgaa agacataact actccgaacc gccccgaccg
     1441 ttaccggtgt agttttcagg gagatactga aaaggaaaat gacaaaaacc acaatgtcat
     1501 gagggagcgg agtgtagcag tttttgtgac gcagcgcata aattatccct gtgactggcg
     1561 tagctaaaat tcttagccat actggacaac tcccctatgg agtcataatc ttaatcaatc
     1621 atttgattaa gaatggactg tgcgatgaat aatcctgcca tgacaatcaa gggtgaacag
     1681 gcgaaaaaac agctgattgc tgccgcactg gcgcagtttg gtgaatatgg aatgaacgcc
     1741 accacccgcg agatagccgc ccaggccggg cagaatatcg ctgccatcac ctactacttc
     1801 ggttcgaaag aagatttgta cctcgcctgc gcccagtgga ttgccgattt tattggcgag
     1861 cagttccgtc cgcacgccga agaggccgaa cgcttgttcg cacaaccaca gcctgatcgc
     1921 gcagccatcc gtgaactgat ccttcgcgcc tgcaggaaca tgattaagct gctcacccag
     1981 gatgacaccg tcaacctcag caagtttatc tcccgtgagc agctctctcc cacggcagcc
     2041 tatcacctgg tgcacgaaca ggtgattagc ccgctacaca gccacctgac acgcctgatt
     2101 gccgcctgga ccggctgcga cgccaacgat acccgcatga tcctccatac tcatgcgttg
     2161 attggtgaga ttctggcgtt ccgtcttggc aaagaaacaa ttctgttacg taccggctgg
     2221 accgcgttcg atgaagaaaa gaccgaactg atcaaccaga cggtgacctg tcatatcgac
     2281 ctgattttgc aaggattatc gcaaaggagt ttgtagtgat gaaaaaacct gtcgtgatcg
     2341 gattggcggt agtggtactt gccgccgtgg ttgccggagg ctactggtgg tatcaaagcc
     2401 gccaggataa cggcctgacg ctgtatggca acgtggatat tcgtacggta aatcttagtt
     2461 tccgtgttgg ggggcgcgtt gaatcgctgg cggtggacga aggtgatgct atcaaagcgg
     2521 gccaggtgct gggcgaactg gatcacaagc cgtatgagat tgccctgatg caggcgaaag
     2581 cgggcgtctc ggtggcacag gcgcagtatg atctgatgct tgccgggtat cgcgatgaag
     2641 aaatcgctca ggccgccgca gcggtgaaac aggctcaagc cgcctatgac tatgcgcaga
     2701 acttctataa ccgccagcaa ggactgtgga aaagccgcac catttcggca aatgacctgg
     2761 aaaatgcccg ctcctcgcgc gaccaggcac aggcaacgct gaaatcagca caggataaat
     2821 tgcgtcagta ccgttccggt aaccgtgaac aggacatcgc tcaggcgaaa gccagcctcg
     2881 aacaggcgca ggcgcaactg gcgcaggcgg agctgaattt acaggactca acgttgatag
     2941 caccgtctga tggcacgctg ttaacgcgcg cggtggagcc aggcacggtt ctcaatgaag
     3001 gtggcacggt gtttaccgtt tcactaacgc gtccggtgtg ggtacgtgcc tatgttgatg
     3061 aacggaatct tgaccaggcc cagccggggc gcaaagtgct gctttatacc gatggtcgcc
     3121 cggacaagcc ctatcacgga cagattggtt tcgtttcgcc gactgctgaa tttaccccga
     3181 aaaccgtcga aacaccggat ctgcgtaccg acctcgtcta tcgcctgcgt attgtggtga
     3241 ccgacgccga tgatgcgtta cgccagggaa tgccggtgac ggtgcaattc ggtaacgagg
     3301 caggacatga atgatgccgt tatcacgctg aacggcctgg aaaaacgctt tccgggcatg
     3361 gacaagcccg ccgtcgcgcc gctcgattgt accattcacg ccggttatgt gacggggctg
     3421 gtggggccgg acggtgcagg taaaaccacg ctaatgcgga tgctggcggg attactgaaa
     3481 cccgacagcg gcaatgccac ggtgattggc tttgatccaa tcaaaaacga cggcgcgctg
     3541 cacgccgtgc tcggctatat gccgcagaaa tttggtctgt atgaagatct cacggtgatg
     3601 gagaacctca atctgtacgc ggatttgcgc agcgtcaccg gcgaggcacg taagcaaact
     3661 tttgctcgcc tgctggagtt tacgtctctt gggccgttta ccggacgcct ggcgggcaag
     3721 ctctccggtg ggatgaaaca aaaactcggt ctggcctgta ccctggtggg cgaaccgaaa
     3781 gtgttgctgc tcgatgaacc cggcgtcggc gttgacccta tctcacggcg cgaactgtgg
     3841 cagatggtgc atgagctggc gggcgaaggg atgttaatcc tctggagtac ctcgtatctc
     3901 gacgaagccg agcagtgccg tgacgtgtta ctgatgaacg aaggcgagtt gctgtatcag
     3961 ggagaaccaa aagccctgac acaaaccatg gccggacgca gctttctgat gaccagtcca
     4021 cacgagggca accgcaaact gttgcaacgc gccttgaaac tgccgcaggt cagcgacggc
     4081 atgattcagg ggaaatcggt acgtctgatc ctcaaaaaag aggccacacc agacgatatt
     4141 cgccatgccg aagggatgcc ggaaatcaac atcaacgaaa ctacgccgcg ttttgaagat
     4201 gcgtttattg atttgctggg cggtgccgga acctcggaat cgccgctggg tgcaatattg
     4261 catacggtag aaggcacacc cggcgagacg gtgatcgaag cgaaagaact aaccaagaaa
     4321 tttggtgatt ttgccgccac cgatcacgtc aactttgccg ttaaacgagg ggagattttt
     4381 ggtctgctcg ggccaaacgg cgcggggaaa tcgaccacct ttaagatgat gtgcggcttg
     4441 ctggtgccga cctccggtca ggcgctggtg ctggggatgg atctgaaaga gagttccggt
     4501 aaagcgcacc agcatctcgg ctatatggcg caaaagtttt cgctctatgg caacctgacg
     4561 gtcgaacaga atttacgctt tttctctggt gtgtatggct tacgcggtcg ggcgcagaac
     4621 gaaaaaatct cccgtatgag cgaggcgttc ggcctgaaaa gtattgcctc acacgccacc
     4681 gatgaactgc cattaggttt taaacagcgg ctggcgctgg cctgttcgct gatgcatgaa
     4741 ccggatattc tgtttctcga cgaaccgact tccggcgttg accccctcac ccgccgtgaa
     4801 ttttggctgc acatcaacag catggtagag aaaggcgtca cggtgatggt caccacccac
     4861 tttatggatg aagcggaata ttgcgaccgc atcggcctgg tgtaccgcgg gaaattaatc
     4921 gccagcggca cgccggacga tttgaaagca cagtcggcta acgatgagca acccgatccc
     4981 accatggagc aagcctttat tcagttgatc cacgactggg ataaggagca tagcaatgag
     5041 taacccgatc ctttcctggc gtcgcgtgcg ggcgctgtgc gtcaaagaga cgcggcagat
     5101 cgttcgcgat ccgagtagtt ggctgattgc ggtagtgatc ccgctgttgc tgctgtttat
     5161 ttttggttac ggcattaacc tcgactccag caagctgcgg gtcgggattt tactggaaca
     5221 gcgtagcgaa gcggcgctgg atttcaccca caccatgacc ggttcgccct acatcgacgc
     5281 caccatcagc gataaccgtc aggaactgat cgccaaaatg caggcgggga aaattcgcgg
     5341 tctggtggtt attccggtgg attttgcgga acagatggag cgcgccaacg ccaccgcacc
     5401 gattcaggtg atcaccgacg gcagtgagcc gaataccgct aactttgtac aggggtatgt
     5461 cgaagggatc tggcagatct ggcaaatgca gcgagcggag gacaacgggc agacttttga
     5521 accgcttatt gatgtacaaa cccgctactg gtttaacccg gcggcgatta gccagcactt
     5581 cattatcccc ggtgcggtga ccattatcat gacggtcatc ggcgcgattc tcacctcgct
     5641 ggtggtggcg cgagaatggg aacgcggcac catggaggct ctgctctcta cggagattac
     5701 ccgcacggaa ctgctgctgt gtaagctgat cccttattac tttctcggga tgctggcgat
     5761 gttgctgtgt atgctggtgt cagtgtttat tctcggcgtg ccgtatcgcg ggtcgctgct
     5821 gattctgttt tttatctcca gcctgttttt actcagtacc ctggggatgg ggctgctgat
     5881 ttccacgatt acccgcaacc agttcaatgc cgctcaggtc gccctgaacg ccgcttttct
     5941 gccgtcgatt atgctttccg gctttatttt tcagatcgac agtatgcccg cggtgatccg
     6001 cgcggtgacg tacattattc ccgctcgtta tttcgtcagc accctgcaaa gcctgttcct
     6061 cgccgggaat attccagtgg tgctggtggt aaacgtgctg tttttgatcg cttcggcggt
     6121 gatgtttatc ggcctgacgt ggctgaaaac caaacgtcgg ctggattagg gagaagagca
     6181 tgtttcatcg cttatggacg ttaatccgca aagagttgca gtcgctgctg cgcgaaccgc
     6241 aaacccgcgc gattctgatt ttacctgtgc ttatacaggt gattctgttc ccgttcgccg
     6301 ccacgctgga agtgactaac gccaccatcg ccatctacga tgaagataac ggcgagcatt
     6361 cggtggagct gacccaacgt tttgcccgcg ccagcgcctt tactcatgtg ctgctgctga
     6421 aaagcccaca ggagatccgc ccaaccatcg acacacaaaa ggcgttacta ctggtgcgtt
     6481 tcccggctga cttctcgcgc aaactggata ccttccagac cgcgccgttg cagttgatcc
     6541 tcgacgggcg taactccaac agtgcgcaaa ttgccgccaa ctacctgcaa cagatcgtca
     6601 aaaattatca gcaggagctg ctggaaggaa aaccgaaacc taacaacagc gagctggtgg
     6661 tacgcaactg gtataacccg aatcttgact acaaatggtt tgtggtgccg tcgctgatcg
     6721 ccatgatcac cactattggc gtaatgatcg tcacttcact ttccgtcgcc cgcgaacgtg
     6781 aacaaggtac gctcgatcag ctactggttt cgccgctcac cacctggcag atcttcatcg
     6841 gcaaagccgt accggcgtta attgtcgcca ccttccaggc caccattgtg ctggcgattg
     6901 gtatctgggc gtatcaaatc cccttcgccg gatcgctggc gctgttctac tttacgatgg
     6961 tgatttacgg tttatcgctg gtgggattcg gtctgttgat ttcatcactc tgctcgacac
     7021 aacagcaggc gtttatcggc gtgtttgttt ttatgatgcc cgccattctc ctttccggtt
     7081 acgtttctcc ggtggaaaac atgccagtat ggctgcaaaa cctgacgtgg attaacccta
     7141 ttcgccactt tacggacatt accaagcaga tttatttgaa ggatgcgagt ctggatattg
     7201 tgtggaatag tttgtggccg ctactggtga taacggccac gacagggtca gcggcgtacg
     7261 cgatgtttag acgtaaggtg atgtaacttc ttatctttcg ccagcaaaga cactaccgcc
     7321 gggccggcaa ggattgccag ccctgcaatt gccagcaaaa agttgttttc cagtacacgg
     7381 gacaacagcc acagcacaat cagcgctgcg ccaaaatacc aggtcgtggt cagctcttcc
     7441 agcacgtctg ccacctcacg ccagcgttgc tcggggtggc gctgaaacac caacatcatc
     7501 actaccgtga cacaatacag tgcgcataat cccacgccga cgtgaaccag agtcttactc
     7561 atccagccca ccaccagcag cgccactacc agtaaatgca acataatctg ccagcaactt
     7621 agaccagttg cgacacgaac acgttgttgc cacttcatgg cttctctcct gaagaattta
     7681 tctctactta ctcagagtac cgaactttac ggaaaattcc gtaagaccgc acctttttgt
     7741 gacgccgact acactatttc aatgagataa gaggcaaaga caggaaaatt atgcccgatc
     7801 aaacacaaca attttcgttc aaggtgctca ccatcaacat tcacaaaggt tttaccgcgt
     7861 ttaaccgacg cttcattttg ccggaacttc gcgacgcggt gcgtaccgtc agcgccgata
     7921 ttgtttgcct gcaggaagtg atgggcgcgc acgaagttca tccgctgcat gtggaaaact
     7981 ggcccgatac ctcgcactac gagtttctcg ccgacacgat gtggagcgat tttgcctacg
     8041 gtcgcaatgc cgtatacccg gaagggcatc acggcaacgc cgtactgtcg cgttatccca
     8101 ttgaacatta tgagaatcgc gatgtttcgg tcgatggtgc ggaaaagcgc ggcgtgctct
     8161 actgccgcat tgtgccaccg atgaccggaa aagcgattca tgtgatgtgc gtacatctgg
     8221 gcctgcgtga ggcgcaccgt caggcgcagc ttgcgatgct cgccgaatgg gtgaatgagc
     8281 taccggacgg cgaaccggta ttggtggcgg gtgatttcaa tgactggcgg caaaaagcta
     8341 atcatccgtt aaaagtgcag gccggactgg atgagatttt tacccgcgtc cacggacgcc
     8401 cggcgcgcac gtttccggtg caatttcctc tactacgact ggacaggatc tacgtcaaaa
     8461 atgccagcgc cagcgcgcca accgcgttgc cgctgcggac atggcgacac ctttctgatc
     8521 atgccccttt aagtgcggag attcatttat gaaatgtagc tggcgcgaag gcaataagat
     8581 ccagttgctg gaaaacggcg agcaatatta tcccgcggtg tttaaggcga ttggcgaggc
     8641 acaagaacgc atcattcttg aaacgtttat ctggtttgag gatgacgtcg gcaaacagct
     8701 gcatgcggca ctactggcag cagcgcaacg cggggttaaa gcggaagtct tgctggatgg
     8761 ctacggttcg ccggatctca gcgatgagtt tgtcaatgaa ctgacggcag ctggcgtggt
     8821 atttcgctac tacgatcccc gccctcgcct ttttggtatg cgcaccaatg tgtttcgccg
     8881 gatgcatcgc aaaattgtgg tgatcgacgc gcgtatcgcc tttattggcg ggctgaatta
     8941 ctccgccgag catatgtcca gctacggtcc agaggctaaa caggattacg cggtacgcct
     9001 tgaagggccg attgtcgaag atattctcca gtttgagctg gaaaacctgc ctggacagag
     9061 cgcggcacga cgctggtggc gacgtcatca caaagcggaa gagaaccgcc agccgggaga
     9121 agcgcaggta ttgctggtct ggcgcgataa cgaagaacat cgcgatgata ttgaacgtca
     9181 ctatctgaaa atgctcactc aggcgcggcg agaagtcatt atcgccaacg cctacttctt
     9241 ccccggctat cgatttttac acgccttgcg taaagcggca cggcgcgggg tgcggatcaa
     9301 actgatcatt cagggcgaac cggatatgcc gattgtcaga gtcggtgcgc gcttgctgta
     9361 taactatctg gttaaaggcg gcgttcaggt atttgagtac cgccgccgcc cgctccacgg
     9421 caaagtggca ttgatggacg atcactgggc gacagtaggg tccagtaatc tcgatccgct
     9481 cagtttgtca ctgaatctcg aagcaaatgt catcatccac gatcgtcatt ttaaccagac
     9541 gctgcgcgat aatctgaacg gcattattgc cgcagattgt cagcaggtcg atgagaccat
     9601 gctgccgaaa cgcacctggt ggaacctgac caaaagcgtg ctggcgttcc actttttacg
     9661 ccacttcccg gcgctggttg gctggcttcc ggcacacaca ccacgtctgg cgcaggttga
     9721 tccgcccgca caaccgacaa tggaaacgca ggatcgggta gaaactgaaa acacgggggt
     9781 aaaaccctga tgagtaaatc acacccgcgc tggcgcttag caaagaagat cctcacctgg
     9841 ctgtttttta tcgcggtgat tgtgttgctg gtggtctacg ccaaaaaagt ggactgggaa
     9901 gaggtctgga aggtcatccg cgactacaat cgcgttgcgc tgcttagtgc ggtcgggctg
     9961 gtggtcgtca gctatctgat ttacggctgc tatgacctgc tcgcccgctt ttactgcggt
    10021 cacaaactgg cgaagcgcca ggtgatgctg gtgtcgttta tctgctacgc cttcaacctg
    10081 acgctcagta cctgggtcgg cggcattggt atgcgctatc gtttgtactc ccgactaggg
    10141 ttaccgggca gcactattac gcggattttc tcgctcagta ttaccaccaa ctggctgggc
    10201 tatattttac tggcagggat tatctttacc gcaggcgtgg tggagttgcc ggatcactgg
    10261 tatgtcgatc aaactacgct gcgtattctc ggcattggct tactgatgat tatcgcggtt
    10321 tatttgtggt tttgcgcttt cgcgaagcac cgccatatga ccatcaaagg gcaaaagctg
    10381 gtgctgcctt catggaaatt cgccctcgct cagatgctga tttccagcgt taactggatg
    10441 gtaatggggg cgattatctg gctgttactt ggtcaaagcg tgaactattt ctttgtactg
    10501 ggcgtgttac tggttagtag tattgctggc gtcatcgtgc atattccggc ggggatcggt
    10561 gtgctggaag cggtgtttat cgcactactg gctggggagc atacctcgaa agggagcatt
    10621 atcgccgccc tgctcgctta tcgcgtcctg tattacttta tcccgctgct gctggcgctg
    10681 atctgttacc tgctactgga aagccaggcg aagaagctac gggcgaaaaa tgaagcggcg
    10741 atgtgaagtt tagttaagtt ctttagtatc tgcatttacg actaatgaaa aaaacgcgta
    10801 tgcctttgcc agacaagcgt tatagctatc gcggcaagta tcccaaagaa gccgataaaa
    10861 tagtaataga gtgttaatgc acctgtaaca gcgcaacgaa ccggactcca caactcttca
    10921 ctatggcatt tccccgccaa ttcaaggagc ttatttcgtc catgcgagat aatgcctgac
    10981 cataacacaa tcattaagca acttattacc caaacgggtc gttcactcat taatgcggca
    11041 ttcatgatga tcaccagggc caaccctgtc aacgtcatca taatgataaa acggtgagaa
    11101 ccaggatcaa cattaaataa taaaccggcc agcatactga ttaaaaacat cgcgcccgca
    11161 gtgctgaaca cgacgataat ggcaggtaca ggagctaaac ttgcaacgac aatcgccacc
    11221 aaagagagta aaaacagata tattacgcct gcgatgacgg catttatctt gttgatatcc
    11281 ttacttaagc catacataca aactaataat aaccctatgg cgacatacat tgttggtgaa
    11341 tcatatttgg ttgccgtaat ttcgagatac ttagctcccc aggccagtcc tgcggttgcc
    11401 accaaaccta aagcggtaat gagccatata ccgtttaata tcctggtctc gtgctgaaaa
    11461 tctaatttat tgctgttttg tgagtatgat tccattcttt tagttgtcct tagaaaaaat
    11521 attaatattc accatcaata aatttaaaac cacgcataca aaatactttt gtatttatgg
    11581 atacggaaaa cgcttccgct cgccataagg cgtattttat acctggtact tgttaagtgt
    11641 cgtttttagt aagaaaaaaa atcccgacac atggccggga tattgatggt gaaaaagaat
    11701 taacggcggt tgccaaaaat tcgcagcaac atcaggaaca ggttgatgaa gtccagatac
    11761 aaggttaacg cgccaagaat ggaatatttg cgcaggttcg acgtgtcgcg ggtatcaatc
    11821 tgctcaccca tatttttcaa tttctgagtg tcatacgccg tcaatccgac aaagacaatc
    11881 acgccgatgt aggtaactgc ccacatcaat gcttcgcttt tcagccagaa gttgaccagc
    11941 gatgccagca caatgccgat taacgccata aacagcatat tgccaaagcc acttaaatcg
    12001 cgcttcgtgg tgtaaccgta caggctcatt gcgccgaaca tcccggcagt aacgacgaaa
    12061 gtactggcga tagaagcagc ggtatagaca atgaatatac tggaaagcgt aagacccgtc
    12121 agcgccgaat aaagcataaa gagcatcgtc gttacacctg cgctcagctt ttgaatcatc
    12181 gctgataaca caataaccaa tgctaattgc gcgatgatca gaccgattaa aaagacacgg
    12241 ttagtaaaca acagctccat cacggccgcg gaattagccg cataccaggc aacaaatgcg
    12301 gtcagcaaca agccaacggt catccagcca tagacttgcg ccatataagt ttgcaagcca
    12361 gcccggggtt gtacgattga atcagaacgt gggaatctgt ccatgacgat ctcctgaaga
    12421 tataaggaat atcttaagga tactgcaaaa tgatgaggct gtgcatcgac gcagcgtaaa
    12481 cgcatgtact gagcggtgaa attgccggac gcagcggtgc cttatccggc taacaaaaaa
    12541 ctaccagcgt tttgccgcct gctgatcgct ctcccgggct tcaacccagc ggtcgccttc
    12601 cggcgtggct tcgcgcttcc agaacggtgc gcgggttttg agataatcca taataaactg
    12661 cccggcttca aacgcactgc tgcgatgcgc actggtgaca ccgacaaaaa cgatttcatc
    12721 gcccggccat aattccccga tgcggtgaat caccgtgacg cgccccagcg gccagcggtt
    12781 acgcgcttca tcaacaattt ctgccagtgc tttttcagtc atccccggat agtgttcgag
    12841 ggttaatgct ttgacgctgt cgcccaggtt atggttgcgc accttaccgg taaaggtgac
    12901 taccgcaccg tcttcgtcac gctccgccag ccacgggtac tcttctccta cgctgaacgg
    12961 ctgcgggccg acaacaattt tggtttctgc catcttaacc tccggttacc ggcgggaaga
    13021 aagctacttc gtcgccgtca gtcagcgaat ggtcaaaact caccagcgtc tggttaacgg
    13081 cagccagcaa tttgccatcc tccagcgcca acgcccagcg atcgctctgc gcagccaggt
    13141 gctggcgtaa cgcttcaacg gttgggaaat ccgcagccac ttcggttgca tctgttccca
    13201 ccaactcgcg cacctgggcg aaaaaaagaa ctttaatcat ccgcttccac cttaaagtca
    13261 cccgacttgc cgccgctctt cgccagcaaa cgtaccggac caatcaccat atctttttgc
    13321 accgctttgc acatgtcata aatggtcagc gccgccacgg aggccgcggt taatgcttcc
    13381 atttcgacac cggttttccc ggtcaggcgg cataaggttt ctatacgcac ccgattgtgc
    13441 tccggctcgg cctgtaaatt gacttcaact ttgctgagca tcagcggatg acagagcggg
    13501 atcagatccc aggtgcgttt tgccgcctga ataccggcaa tacgcgcagt ggcaaatacg
    13561 tcgcctttgt ggtggcgacc atcaataatc atcgccagcg tctcgctgcg catggtgaca
    13621 aaggcttccg cccgcgcttc acgcacggtt tccgctttgg cggagacatc caccatgtgc
    13681 gcttcgccag cggcgttgat atgggtcagt tgcgacatac ttatttcttc aaatgtggat
    13741 ggaaattaca cggacgcgta cgggcatcca gctgcggcgc gatgatattt tcccatgcgg
    13801 tacggcacgc tttggtcgaa cccggcatgg cgaaaatcag cgttttgttg gcgacgcccg
    13861 ctaccgcacg agattgcaac gtggaagtgc caatctcttc aaacgacaac atacggaaca
    13921 cttcaccaaa accttcaatt tcacggtcga acaacggcag caatgcttcg ggagcctgat
    13981 caccttccgt caggccagta ccacccgtaa tcaataccac ttgtacatcg tcgctggcga
    14041 tccacgcaga tacctgagcg cgaatagcgt agcggttttc tttcacaatg gctttatcga
    14101 caacgtgatg gcccgcttct tgcgccgaat cgcgcagata gtgaccggag gtatcgtctt
    14161 cttcaccgcg acgattagaa accgtaagaa tagcaatacg ggtcgggata aattcagtgc
    14221 ttacctgact catctgatct ctccttttga cgttttagcc gccaatgtac gataagtttt
    14281 gcgtaatacc ggtgttgttt tgatgcagga aatgggtctg tttcttctcc cgcagcgccg
    14341 ctgaaatacg cgcttccagc gcctgttgct gggtatcgtc ttccagcaga tcgcgcaggt
    14401 taacgccgcc ttcaccaaac aggcagagat ggagtttacc aatggaggaa acgcgcaggc
    14461 ggttgcaagt ggcgcagaag tctttttcat acggcatgat aaggccaatc tctccggcgt
    14521 aatctggatg gcaaaagact tgcgcgggac cgtcgctgcg ttgacgtaat tggtggatcc
    14581 agccgcgacg cagtagctcg tcacgcagaa cctgaccaga gatgtgatgc ttacggaaga
    14641 gctcgctgcc ctcgcccgtt tccatcagtt cgatgaaacg cagctggata gggcgatgct
    14701 ggatccagtt cagaaaggtg tcgagctggt gatgattaac atcacgcatc agcacggtat
    14761 tgactttgac cttctcaaaa ccggcctcaa atgcagcatc aatccccgcc atgacctggt
    14821 tgaatttatc ctgcccggta atagcgtgaa actggcgggc gtccagactg tcgacactga
    14881 cgttaatgcc agtaagtccc gcatcgcgcc agttcgccac atcgcgttcc agacggtaac
    14941 cattggtggt gaccgcaatc tggcggatag cgtcgttttc ccgcacagcg gcgatgatat
    15001 cggtaaagtc gcggcgtaaa gacggctcac cgcctgtcag gcgcactttt tcggtgccca
    15061 gactggcgaa ggcgcgcgta acccggcgaa tttcatcgac ggtaagaaag cctttattgg
    15121 tgacgccgct cggtttgtag ccatccggca ggcagtaggt gcaacgaaag ttacacacat
    15181 cggtaatcga caggcgcaag tagtaaaact tacgcgcaaa tgcatcagtc agttgtgaag
    15241 ccatgtacac ctttccagat acgggaggcg aagtcatttc ttccttcgcc ctggtggcaa
    15301 tctttacgag cattgtcacg gccaaagcac cgtatcagtt gacccaggtg cagaggctag
    15361 agtgtttagt ggttatgccg atactagcgt gtaaatgaga tttttaccat ccacactttc
    15421 gctatgtaat catgtatata gcgtcatgat cgtgctattt cgctacagaa taagggaaaa
    15481 tggcggtttt actttgatat acatcatcag ggaggcatag cgtgcatcgt cctttagagg
    15541 tagaagaaaa tagtttgtat cctcaatatt ggcaggttaa ttgctgtttc cccgcaattt
    15601 gcgctactgt agcgcggata atctgactcc aggaaactat atgcgcaatc gtacgctggc
    15661 tgatcttgat cgtgtcgttg ctctcggcgg agggcatgga ctgggacgcg ttctctcatc
    15721 actttcgtct ttgggttctc gtttaacggg tatcgtcacc accaccgata atggtggctc
    15781 gacggggcgt attcgccgtt cagaaggcgg cattgcctgg ggcgatatgc gcaactgcct
    15841 caaccagctg ataacggaac cgagcgtcgc ctccgcgatg tttgaatacc gttttggtgg
    15901 caatggcgaa ctttccggtc ataatctcgg aaacttgatg ttaaaggcgc tggatcacct
    15961 tagcgtgcgg cctctggaag ccatcaattt aattcgtaat ctgctgaaag tggatacgca
    16021 tttgattcca atgtcagagc atcctgttga tctgatggcg attgacgatc aggggcatga
    16081 agtttacggc gaggtcaata tcgaccagtt aactacgccg attcaagagt tattgttaac
    16141 gcctaatgta cccgcaacgc gtgaggcggt tcacgctatc aatgaagcgg atctcatcat
    16201 tattgggcct ggcagttttt ataccagcct gatgccaatt ctgctgctga aggaaatcgc
    16261 ccaggcatta cgccgcacgc cagcgccgat ggtttatatc ggcaatctgg ggcgtgagtt
    16321 gagtttacct gcggctaatt tgaagctgga aagcaagctg gcaattatgg agcagtatgt
    16381 tggtaaaaag gtcattgatg cggtcatcgt cgggccaaaa gtggatgtct cggcggtgaa
    16441 agagcggatt gtgatccagg aggtactgga ggccagcgat attccgtatc gtcatgaccg
    16501 ccagttgtta cataacgcgc tggaaaaggc gttacaggct ttaggttaac ggaaatccga
    16561 aggaaaattc cggcttccta ttgaagacaa agtgcgcgtt gtttatgccg gatgcggcgt
    16621 gaacgcctta tccggcctac aaaccgcgca aattcaatat attgcggaga aaatgtaggc
    16681 ctgataagcg tagcgcatca ggctgttttc cgtttgtcat cagtcttctt cgctatcctg
    16741 ttacgatgcc gcgataaaca gctcacgcag ctgatgcaac tggtcacgaa tttgcgccgc
    16801 ttcttcgaac tccagattct gcgcgtgttg catcatcaac ccttccagct catggatttt
    16861 ctgctgcaac gctttaggcg acatatccat cggcacatta tccggctcaa caatcggtcg
    16921 cgattttcct ctgcccttcg ctttggtttt agcaatgttc tgccccaacg ccaggatatc
    16981 gaccactttc ttgttcaagc cttgcggcgt aatgccgtgt tcctcgttgt acttctgctg
    17041 tttctcgcgg cgacgttcgg tttcgccaat cgctttcgcc attgatgggg tgatcttatc
    17101 gccgtagaga atcgctttac cgttaacgtt acgtgccgca cgaccaatgg tctggatcaa
    17161 cgaacgttcg gaacgcagga agccttcttt gtcagcgtcg aggatcgcca ccagcgaaac
    17221 ttccggcata tccagacctt cacgcagtaa gttgatccct accaatacgt cgaactcacc
    17281 cagacgcaag tcgcggataa tctccatacg ttcgacggtg tcgatatctg agtgaagata
    17341 acgcacgcgc tcgccatgtt cttcgagata ttcggtaaga tcttccgcca tccgcttggt
    17401 cagtgtggtg accagtacgc gttcgttaat tgccgctcgc tgacgaatct ccgaaagaag
    17461 atcatcaacc tgtgtcgcca ccggccgcac ttcgataatc gggtcaagca atccggttgg
    17521 acgcaccacc tgatccacca catcgccgcc ggatttttcc agctcgtaat tacccggcgt
    17581 cgccgaaaca tagatggttt gcggcgctaa tgcttcgaac tcttcaaact taagcggacg
    17641 gttatccagc gctgatggca ggcggaagcc gtactccacc agtgtctctt tacgcgcccg
    17701 gtcaccgcga tacatgccgc caatttgtgg aatggtgacg tgagattcat cgaccaccag
    17761 cagcccatca gcagggaggt aatcaaacag cgtcggcggt ggctcacccg gtccacgacc
    17821 ggagaggaag cgcgagtagt tttcaatccc cgaacagtag cccagctcgt tcatcatctc
    17881 cagatcaaac tgggtacgct gggtcagccg ctgctcttcc agcagtttgt tgttttccaa
    17941 cagcactttg cgtctggcgg ccagctcttc tttgatctcc tccatcgcct gtacgatgcg
    18001 ctcgcgcggt gtgacgtagt gcgttttcgg gtagatggta aaacgtggaa tagtggaaac
    18061 aatctgcccg gtcagcgggt caaataacga caatcgttcc acttcctcgt caaacagttc
    18121 cacgcgaagt gcaatgtcat ccgattctgc cgggaagata tctatcacct cgccacgaac
    18181 gcggaaagta ccacgctgga atgcttgatc attacgagcg tattgcagct ccgccagtcg
    18241 gcgcagaatc gcgcgctgat cgataatcat accgaccgtg agatggagca tcatcttgag
    18301 atataaatca ggatcgccca gaccataaat cgcggaaaca gacgccacca caaccacatc
    18361 acgccgctcc agcatcgctt tggtggcgga caaacgcatc tgctcaatat gttcgttaac
    18421 cgaggcatct ttctcaatga aagtgtcgga actcggtaca taggcttccg gctgatagta
    18481 gtcgtagtag gaaacgaaat attccaccgc gttttccggg aagaactctt tcatttcgcc
    18541 atacagctgg gccgccagcg ttttgttggg cgcaagtacc atggttgggc gctgaaggtc
    18601 agcaatgaca ttggcaatgg tgaatgtttt cccggagcca gtcacgccaa gtaacgtctg
    18661 gtgcgccagg ccatcttcca gcccctcttc gagacgtcga atcgcctctg gctgatcgcc
    18721 agaaggttta aaagcggaat tcagtttgaa cggtttactc atgagtcgct acctgaagga
    18781 gttgggcggg caggtatgta attttactcg tcgtacttaa ttatgccaac aaattatact
    18841 ggataaaaaa acagttcatc accataatat ttctgttaca gcgtaaactc cgctgtcaat
    18901 catgagcaaa atttactctg tggcgagata aaactccggc cttaccgggt tatccccaaa
    18961 gcaacggctt ttttaacatt tgtcaagatg agtgatgaca gttttatggc aagagatgcc
    19021 tgttcagtga ctgccattgc atttatctaa ccagttaaaa aataaaagat atttctttga
    19081 gccgtcttta acgccaattc gcgtgcaagc cgcgtattct ctcgcttgcc tcgtgttttc
    19141 taactctaat acacatggtt atccacagga atagtggata actgtctcca gcccctatcc
    19201 cccgccgcct gggcaattcc cacattcgcc gcatgaggcg gaagtaaaat ttttttcgtg
    19261 attattttag aatttaattg gttaaattgc agtcaatcga agacgcgatc tcgctcgcaa
    19321 tttaaccaaa tacaggatag ctacaacaag gcaaggttta tgtactttcc ggttgccgca
    19381 ttttctggat tttctgcaag ccaggggatc tctcccagca gcggcgcggg aatcatgcgg
    19441 gtgagcgtgg tcatatattc agcgtgacgt tttcccggag gcgtaacatc gttcgccacc
    19501 caacccgcca gagtcagtcc ggcgtgttgt attgcctgcg cagtcaacat cgcgtgatta
    19561 atacagccga gtttcacacc aactaccagt atcaccggca gttgttcctg tgttacccaa
    19621 tctgcaaaag tgaaagtgtc agaaagcggc gtaaaccagc cgccagcacc ttccactaac
    19681 acccagtcag cctgttgttc aagcgcgcgt aatccggcgc tcattaccaa tgattctatc
    19741 ggcctgccct cttgcgcgct gatgatgtgc ggcgaggtgg gttctgcgaa ggtgtaagga
    19801 tttaccgttg cgtaatccag ctgcaggctg ctgttgtgct gtaacgccag cgcgtcacta
    19861 ttacgtaagc cttccggggt cttttcgctg ccagaggcga ccggtttata acctgccgtc
    19921 cgataaccta ctgccttcgc ggcttgtaaa agtgcacaac tggcgacggt tttccccact
    19981 tcggtatccg ttccggtgac aaaataacgt ttactcacga gcaatcactc ccaaaaaaag
    20041 atgatacgtc agaggatatc gcccctgttg ttgcggccag gccagttgca atcgctgcaa
    20101 ttgcgaacgc gttaatattc gcgggtcgcg cccttcatga agatgcgtag caccgatgcc
    20161 tttcagcgaa cgcatggcac tgagcgcatc atcaaaccat agtgtgatgg gctgaatatg
    20221 atgttgataa tgcacgccgt tcagcgactg ttcgatttca tctggcggta aaaagcgatt
    20281 agcatgcgga cgctcgtcca ccgcctgcca cgcctgatgc agttcgggta acgatccctg
    20341 ctccagcgtg gtaaacgcca ccacgcctcc ggggcgcacc acccgataca gctcgcggag
    20401 tgccgtggat aaattaccgc accactgcac tgcgagattg ctccatgcaa gatcgaacgt
    20461 cgcagtcgct aacggcaggg attcgatatc tcccgccaga taatggtctg cggcatcctt
    20521 ctggcgtgcc tgaacaagca ttggcggcga gagatctaag gccgtcacct gcgcgtgacg
    20581 ttcccgccag tggcggctca tccagccagg tccacaaccc gcgtccagta cgtgggtgta
    20641 tttacgctgt gggagcattg ccagtaagac gtcagcactc tggcgctgta gatctgcgtg
    20701 ttgctcatag agtgcggctg cccgaccaaa tgccgctgca atggcttgtt tattaaccgt
    20761 tgccatgcag cacctccagc agacggtcga tatcctgcat ttcatgcgct gcggttagcg
    20821 ttaagcgcag tcgcgcagta ccagcgggta cggttggcgg gcgaatcgcc gtgacccagc
    20881 agccttgctg gcgcagtttt tctgccagtt gtaacgcacg gctgttatca ccgacgatca
    20941 atggctggat ggcgctgcat gaatcagcca gcgtaaacgg caaatcctgt actccggcac
    21001 gaaaacgcgc aatgagtgac accagttttt cgcgccgtgc atccccctca tcacggcgaa
    21061 tgaccgccag tgacgcacgt aatgcctgcg cctgagcggg cggcatactg gtgctgtaga
    21121 taaggtggcg ggcgaattgc agcagataat ccgccaccgt acttgagcaa agcaccgcag
    21181 ccccactgac gccaaatcct ttgccaaaag tcactaccag caattctggt tttacctttt
    21241 gcagccagca gctgccgcgc ccctgctccc cgataacgcc cgtgccgtgg gcatcatcga
    21301 ccatcaacca gccattgtgc tgttgcgtta cctgctggat ttccgccagt ggtgcactat
    21361 cgccgtccat gctgaacacg ccttctgtca ccaccagttg ctgccccgga cagggggaag
    21421 caagcaatcg cgccaaatga gtgacatcgt tatgagcaaa acggcgaagc tgcgacgggc
    21481 ttaaactggc agcttccagc aatgaggcat ggctaagccg gtcagcgaca atgcggtcct
    21541 cttttgccat catcgcggca atgaccgcct gattagcggc aaaaccagag ataaatagca
    21601 gtgcccgcga atagcccagc cactcggcca gttcttcttc cagcgcctga tgcgcaacgc
    21661 tataaccgct gacgtgaccg gaaccgccgc taccgacgcc aaattgctcc gcaccctgct
    21721 gccaggcacg gataatttgc ggatgatggc ttaaacctaa ataatcgtta ctggaaaagt
    21781 tcagatactg gcgatcatcc gccaccagcc agcgtccggc tccttgcgcc actggataac
    21841 ggcgacgcag ggcatcggca gcacgccgcg catcgagcgc cgcgttgatt ttctcctgcc
    21901 agctcatagt gctgccgcgt tgtaatattc gtcagtgtcc ggggtcatca gtgcctgctc
    21961 cagacgctgc tgttgttcgt tatcccctgc cagcacggca gtttgctgcg gatttagccc
    22021 cagtttgtga aacagttgca ggtctttatc ttcttccgga ttcggcgtgg ttagcagttt
    22081 gcaaccgtag aaaatcgagt ttgcgcctgc cataaagcac atcgcctgag tctgttcgtt
    22141 catctgctcg cgtccggcag aaaggcgcac gtaagaggtc ggcatcatga tccgcgcgac
    22201 cgcaatggtg cgaataaaat caaaggcatc gacatcatcg ttatccgcca gcggcgtgcc
    22261 tttcaccttc accagcatgt tgattggtac gctttccggc ggcgtcggca ggtttgccag
    22321 ttgtagcaat aatccggcgc gatcttttac cgtttcgcct aagcccacaa tgccgccaga
    22381 acagactttg atcccggcat cgcgcacttt ttccagcgta tcgaggcgtt cctgataagt
    22441 gcgggtggtg atgatattgc cgtaaaactc cggcgaggtg tccaggttgt ggttgtagta
    22501 atccagcccg gcgttcgcga ggcgctgcgc ctgagattca ctcaacgtgc ccagcgtcat
    22561 acacgcctcc agccccatcg cttttacccc ctgcaccatt tgttccaggt acggcatatc
    22621 gcgttcgtgg ggattcttcc acgccgcgcc catacagaaa cgcgtcgatc ccgcagcttt
    22681 cgctttgcgc gccgactcca gcacctgttc aacttccatc aaccgctcgg cttccagccc
    22741 ggttttgtag cgcgagcttt gcgggcagta tttgcaatct tccggacaag ctccggtctt
    22801 aatcgacagc aaagtgctga cctgcacctg acgaggatcg aaatgctgtc gatgcacctg
    22861 ctgcgcttca aacagcagat ccagcaacgg tttttcaaat aattctgtga cttgcgacaa
    22921 tgtccagcgt gggcggtgag ccatggggct tctccaaaac gtgttttttg ttgttaattc
    22981 ggtgtagact tgtaaaccta aatcttttca atttggttta caagtcgatt atgacaacgg
    23041 acgatcttgc ctttgaccaa cgccatatct ggcacccata cacatccatg acctcccctc
    23101 tgccggttta tccggtcgcg agcgccgaag gttgcgagct aattttgtct gacggcagac
    23161 gcctggttga cggtatgtcg tcctggtggg cggcgatcca cggctataat cacccgcagc
    23221 ttaatgcggc gatgaagtcg caaattgatg ctatgtcgca tgtgatgttt ggcggtatca
    23281 cccatgcgcc cgccatcgag ctatgccgca aactggtggc aatgacgccg caaccgctgg
    23341 aatgcgtttt tctcgcggac tccggctccg tagcggtgga agtggcgatg aaaatggcgt
    23401 tgcagtactg gcaggccaaa ggcgaagcgc gccagcgttt tctgaccttc cgcaatggtt
    23461 atcatggcga tacctttggc gcgatgtcgg tgtgcgatcc ggacaactca atgcacagcc
    23521 tgtggaaagg ctatctgcca gaaaacttgt ttgctcccgc cccgcaaagc cgcatggatg
    23581 gtgaatggga tgaacgtgat atggttggct ttgcccgcct gatggcggcg catcgtcatg
    23641 aaatcgcggc ggtgatcatt gaaccgattg tccagggcgc aggcgggatg cgcatgtacc
    23701 atccggaatg gttaaaacga atccgcaaaa tgtgcgatcg cgaaggtatc ttgctgattg
    23761 ccgacgagat cgccaccgga tttggtcgta ccggcaaact gtttgcctgt gaacatgcag
    23821 aaatcgcgcc ggacattttg tgcctcggta aagccttaac cggcggcaca atgacccttt
    23881 ccgccacgct caccacgcgc gaggttgcgg aaaccatcag taacggcgaa gccggttgct
    23941 ttatgcatgg gccaactttt atgggcaatc cgctggcctg cgcggcagca aacgccagcc
    24001 tggcgattct cgaatctggc gactggcagc aacaggtggc ggctattgaa gtacagctgc
    24061 gcgagcaact tgcccccgcc cgtgatgccg aaatggttgc cgatgtgcgc gtgctggggg
    24121 caatcggcgt agtcgaaacc actcatccgg tgaatatggc ggcgctgcaa aaattctttg
    24181 tcgaacaggg tgtctggatc cggccttttg gcaaactgat ttacctgatg ccgccctata
    24241 ttattctccc gcaacagttg cagcgtctga ccgcagcggt taaccgcgcg gtacaggatg
    24301 aaacattttt ttgccaataa cgagaagtcc gcgtgagggt ttctggctac actttctgca
    24361 aacaagaaag gagggttcat gaaactcatc agtaacgatc tgcgcgatgg cgataaattg
    24421 ccgcatcgtc atgtctttaa cggcatgggt tacgatggcg ataatatttc accgcatctg
    24481 gcgtgggatg atgttcctgc gggaacgaaa agttttgttg tcacctgcta cgacccggat
    24541 gcgccaaccg gctccggctg gtggcactgg gtagttgtta acttacccgc tgatacccgc
    24601 gtattaccgc aagggtttgg ctctggtctg gtagcaatgc cagacggcgt tttgcagacg
    24661 cgtaccgact ttggtaaaac cgggtacgat ggcgcagcac cgccgaaagg cgaaactcat
    24721 cgctacattt ttaccgttca cgcgctggat atagaacgta ttgatgtcga tgaaggtgcc
    24781 agcggcgcga tggtcgggtt taacgttcat ttccactctc tggcaagcgc ctcgattact
    24841 gcgatgttta gttaatcact ctgccagatg gcgcaatgcc atctggtatc acttaaaggt
    24901 attaaaaaca actttttgtc tttttacctt cccgtttcgc tcaagttagt ataaaaaagc
    24961 aggcttcaac ggattcattt ttctatttca tagcccggag caacctgtga acacattttc
    25021 agtttcccgt ctggcgctgg cattggcttt tggcgtgacg ctgaccgcct gtagctcaac
    25081 cccgcccgat caacgtcctt ctgatcaaac cgcgcctggt acctcttctc gcccgattct
    25141 gtcggcaaaa gaagcgcaga atttcgatgc tcaacactat tttgcatccc tgacaccagg
    25201 tgctgcagcg tggaatcctt ccccgattac cctgcctgcg caacctgact ttgttgtcgg
    25261 cccggcgggc actcaaggtg taacgcatac cacgattcag gcggcggtag atgcggcaat
    25321 tatcaagcgt accaacaagc gccagtatat tgccgtgatg cctggtgagt atcagggaac
    25381 ggtatatgtc cctgccgctc cgggtggaat tactctgtac ggtacaggtg aaaaaccgat
    25441 tgatgtgaag attgggcttt cccttgatgg tggcatgagc cctgccgact ggcgtcacga
    25501 cgtcaacccg cgcggcaaat atatgccagg taaaccagcg tggtatatgt acgatagctg
    25561 ccagagcaaa cgcagcgaca gtatcggtgt tctctgctct gcggtcttct ggtcacaaaa
    25621 caatggcctg caactgcaaa atctgaccat cgaaaacacg ctgggcgata gcgtagatgc
    25681 aggtaaccat ccggcggtgg cactgcgtac tgatggtgac caggtacaga ttaacaacgt
    25741 taacattctc ggtcgtcaga acaccttctt tgtcaccaac agcggtgtgc agaaccgtct
    25801 ggaaaccaac cgtcagccgc gtacgctggt gaccaacagc tacattgaag gggatgtgga
    25861 tatcgtttct ggtcgcggcg cagtggtgtt cgataacacc gaattccgcg tggtgaactc
    25921 acgtactcag caagaagcgt atgtgtttgc accggctacg ctgtccaaca tttactacgg
    25981 tttcctcgcc gtaaacagcc gtttcaatgc tttcggtgat ggtgtggcgc aactgggccg
    26041 ctcgctggat gttgatgcca ataccaacgg tcaggtggtg atccgtgata gcgccatcaa
    26101 cgaaggtttt aacacggcta aaccgtgggc cgatgcggtg atctctaatc gtccgtttgc
    26161 gggtaatacc ggcagcgtag atgataacga cgaaatacag cgcaatctga atgacactaa
    26221 ctacaaccgc atgtgggaat acaataaccg cggcgtgggt agtaaagtgg ttgcagaggc
    26281 gaagaagtaa gagcaattaa ctatttgccg gatgcggcgt aaacgcctta tccggcctac
    26341 ggttcgatgc gatttgtagg tcggataaga tgcgcaagca tcgcatccga caataagtgc
    26401 cggatgctgc gaaaatgcct tatctggcct acagattcga tacgattcgt aggtcggata
    26461 agatgcgcaa gcatcgcatc cgacaataag tgccgaatgc gacctacatt cacatggcgc
    26521 tttttacatc tgacggtttt tattgaagtt aatcaaacta cccgccttga taatctcgcg
    26581 ctcttcagca gtcagacttt ccatatagag cgtaatttcc gttaccggcg catcttcatg
    26641 gatcacataa cctttaaacg tcgtacccgg attatccagc gccgctttaa tgccagggat
    26701 gtaaatgtaa tcccccactt caaaggttgg cacttccgcc atctgcagcg gtaacatccc
    26761 ccagttgatg acgttagaac ggtagcgttt agtcgcgtac tcttcggcaa tattcgccag
    26821 accgccaatc acacgctggc agctcgccgc ctgttcacgc gcagaaccat cgcctggttt
    26881 caccgcatat accatgctgc caatttcagt ttgcagcgga tcaatatgct cctgaccagc
    26941 aatctgctta atgcgcgcaa acacctctgt cagctcgctg acattccccg ccagacgctg
    27001 attttccagc tcagcagtcg ctttacttct gccaacataa ccgggatcgc ggcgtgacag
    27061 ggtaaactcc gccagaccaa tcggatttga acgataagaa gaggtttcac cggaaggaat
    27121 cagttcgtcg gtggtggtca cttcgtcgag gatcttcgag cacactttca ggacgatatt
    27181 gtcagtcagc gcacccaatt ccggccagtc tttaatgttc ggtccgtaaa tcagcggttg
    27241 ctgagttgcc cctttcacaa agccctgata aacacggttt ttatacggcg ttacatcgaa
    27301 ggcgtactcc ggcacgttgt cccagcaatc aagttcgctg gcagaggtta aatagccacc
    27361 gtttgccgca gtcgcagcga tagaacgagc gtccatcaac gccaccgctg acatctgccc
    27421 attagctggc ttagagcctt cgcggttcgg gaagttacgc gtggtgtggc gaatactcaa
    27481 accgttgttg attggcgtat cgcccgcgcc aaagcatggg ccgcagaacg cggttctgat
    27541 gattgcgcct gcgccaatca aatctgctac cacacctttt ttggcgagat ccataaacac
    27601 cggctgtgat gacgggtaaa ctgccagcga gaaggtgtca ttgccacagg attgaccgcg
    27661 cagtgcattc gccgccgcga tgacgttttc gtagttaccg ccagaacagc ccgcgataat
    27721 cccctgctgc actttcaggc gaccattttc cactttatcc agcagcgaga gtttggcttt
    27781 accgtgcgcc acgcgttcgg actcaatttc aatctcacgc agaatgtcgg tcaggttctg
    27841 gttcagtgtg tcgattttat acacgttgct cgggtggaac ggcagcgcaa tcattggttt
    27901 gatggcgctt aaatcaacgc tgatgcagcc atcgtagtac gccatcggtt gagggttaag
    27961 ctggcagtaa tcctggccgc gaccgtgcag cgccagccag ttatgcactt cttcatcagt
    28021 ttgccagacg gagcttaaac aggtggtttc cgtggtcatc acgtcaacgc tgttacggaa
    28081 atcggtagag agcgcgctaa cgcccggtcc aacgaactcc atgactttgt ttttgacgta
    28141 accgtttttg aacaccgcgc caatgatagc cagcgccaca tcctgtggcc ccacatacgg
    28201 cgcaggtttt ccggtcagat gcaccgcaac cacgcccgga tagtcgatat cccaggtgtc
    28261 attaagcagc tgttttacca actcaccgcc gccctcaccg actgccattg tccctaatgc
    28321 accgtaacgg gtgtggctgt ctgacccgag gatcattttg ccgccgcctg ccatcatctc
    28381 acgcatatat tgatggatga ccgcaatatg cggaggcaca aaaataccgc cataacgctg
    28441 ggccgccgat aaaccaaaaa catggtcatc accgttaata gtgccgccga cggcgcagag
    28501 tgagttatgg cagttggtca gcacatacgg cagcgggaaa cgttccatac cggacgcttt
    28561 agcggtctgt acaataccaa caaaggtaat atcgtgagag gctaatgaat caaacttaat
    28621 tttaagttta tccatatttc cggacgtatt atgcgaagag agaatagacc aggcaatagt
    28681 gccttttttg gcttcttctt ttttaatttc gccggtgaaa tgttcttcgg caattatttc
    28741 gttattactg gcgagaaaca cgcctttttc agataacttg atcatcaaat gctccagata
    28801 tctgcgcacc gtgaagtgcg cagaaagata ataaatggat atactctaaa taattcgagt
    28861 tgcaggaagg cgacaagcga gtgaatcccc aggagcttac ataagtaagt gactggggtg
    28921 agcgaacgca gacgcagcac atgcaacttg aagtatgacg agaatattta cagcatgttc
    28981 cagccgatca gcatattcca ccaccacacg ccgagggtga tatgcaccag gaaggttaat
    29041 atcgtcagta ccgcaccgac caaccaccag gatttaatat cgttataacc cacgccaaag
    29101 atgaccggac ctgccgcgcc gccatagtga gtaaccatgc cgccatagga gttggagaac
    29161 aacagtgcca gcgcggttaa cattaacggt gcgccggaga cgttcgccag catggcaaat
    29221 accggtaaca tagcaacgat ataggcacta ccggaagcga agaaataacg cacgataatg
    29281 ctgaggaaaa taataacgaa gaaagcaacg ttaccgtgac catcaaatgc caggttattt
    29341 ttaaagactt cagctaacca ttcgaagaat ttaactttcg ataataagga gcttaatccg
    29401 ataataccgc cgtaccagat taaggtattc cagccgcctt tatttttaac cacgtcttcc
    29461 caggtaacga tacccagcag cagcatggtc gccataacaa cgattgccac agtggattca
    29521 tcaaccccca gagacttact gaaaatccag cccagtagcg ccagcacaaa gacaccgagc
    29581 agcatttttt cgcggatttt catcggtccc agttcggcaa ggcccgcttt agcgatggtt
    29641 ttattatcca ccttcttaat ttctggcgga tacatggtat aaatcaccag tggggtgacc
    29701 agcagcataa tgatgcccgg caatccggcg gccagtgccc atccacccca gctaatttgc
    29761 aggtgcagaa tgtcgttgat cattttcagc gccagaatgt tccccgccat tgcggtaaag
    29821 aacatatagc tggtggtttt ggtgaccatg taaatggaca tcatcaggta atgtccgaca
    29881 cgacgcggac ttttttccgg ttcggacccc aaagccaccg ccacgctgtt gatgatcggt
    29941 aacacaatac cgcccgcacg cgcggtgtta gacggtgttg ccggagccag taccagatcg
    30001 aggaataccg taacgtaacc cagacccagc gtggtgttac cgattttacc aatcagcaga
    30061 taggcaatac gtttacctaa accggtggtc acaaatgcgg cgcttaaggt aaacgccgag
    30121 aacaccagcc aggtagtacc tgaagagtaa ccgcttaata cggcggtggt cttaaacgca
    30181 ccgtcggata agttaccgac caccaccatg gaggcagcaa cggcaattaa cagtacgaca
    30241 ggttccggga aaggcttgat aaccagcccc acaatggccg ccaggtaaat accaaaaagc
    30301 acccacgcca gttcgcttaa tcctgccgga gctggcataa aaccaataat acatgggatc
    30361 gctaatatca gaattagctt ccataacgat ttcttattca tttaaaatcc tgttctttct
    30421 gtatgactca tacagaagtc ctttattgat tatccttatc ttgacaacga attttttcag
    30481 ggaagataaa cttcaccgga aaatattttt ctggtcgtcc gaataacaga tgcgcgtaac
    30541 gtcgtggcat cctgaccttc attacttaaa tgaacgtcga gcgcaccact ggggtgttca
    30601 atattgatat tgccgtatcc tacagaaggg acgatttgtc gggtgacggt gccttccaat
    30661 gcacaactac tggaaatagc aatagcaccg gttatcgcca gcgcgcgatg gcaagaatgc
    30721 ggcataaaat aacgcacatt aattgccccg cctttctgcg ctggagaaat aagcacaggt
    30781 ttagggataa ccatattact gacatcacct aagcccattg ctttacccgc ttgtagacgg
    30841 atagattcaa tgcgggctaa taatgctttg tcggcatcca gttccgccgg taattcataa
    30901 cctgtttttc ccagatattc agccggaata atgacgactg gcatcgccat atcgatacag
    30961 gtcaccggga catcgtcaaa ataatcaatc tgattatcag tcgggaaaac ttttccggtt
    31021 ttggttccag cggcattcag gaaagtgagc gcaaccggtg cggcagtacc cggtacgccg
    31081 tcaattctgg cactaccctc gtactcgaca acaccatttg gcgtttgcac atcagcttcg
    31141 atgaacgtac ccgtattgac gttgcggata cgcacacggg tgaccggtga tgtcgctgca
    31201 atcaaaccat tttcaatggc gaatgcccca acaccagaca gcatattgcc acaattaggc
    31261 gtggtatcga cacgttgctc atggacaata acttgagcaa acagataatc gacatcagca
    31321 cgcggatcgc tggaacggct aataatggcg actttactgg tcagcggatt accgccgcca
    31381 ataccgtcaa tttccagatc gttaccggaa cccataattg ccatcaatat tttatcgcgc
    31441 tgcgtttgat cttcgggtaa atgttccgct aacaagaacg cgcccctcga ggttccacct
    31501 cgcatcatca cgcagggtat tttttcataa taatgccctc aatttaaaaa cataacttat
    31561 tgcgatatgg ttacattaag gacaaagcat ctctaatgac tttagctcaa cgattaataa
    31621 cattttttca ttaatttaag ttggctaaat gcagttaaaa tttttaacga ccagccaccc
    31681 aaaatcgtcg cttccatttt ccggagtaat aatgaagcat gaattatcaa gtatgaaggc
    31741 atttgtcata ctggcagagt ccagttcatt taacaatgcc gctaaattac tcaatattac
    31801 gcaacctgca ttaacgcgaa gaataaaaaa gatggaggaa gatttacata ttcaactatt
    31861 tgagcgtaca actcgcaagg ttacgttaac aaaagcagga aaaaggttgc tcccggaggc
    31921 gcgggaatta ataaagaaat tcgatgaaac gctttttaat attcgtgata tgaatgctta
    31981 tcatcgtggt atggtgacgt tagcgtgtat tccgaccgca gtattttatt ttttaccgct
    32041 ggcaatcggt aaatttaatg agctgtatcc caatattaaa gtgcggattc tggaacaagg
    32101 cacgaataat tgcatggaat cggtgctgtg taacgaatca gactttggca taaacatgaa
    32161 caacgtcaca aattcatcca ttgattttac cccactggtc aacgagccat tcgtgctggc
    32221 ctgtcggcgt gaccatccat tggccaaaaa gcagcttgta gaatggcagg agttagtggg
    32281 ttacaaaatg attggcgtac gttcctccag cggcaaccga ttgttgatag aacaacagct
    32341 agccgacaag ccctggaagc tggactggtt ttacgaagtg cgtcatcttt cgacgtcatt
    32401 aggactagtg gaggcaggac tggggatttc ggcgcttcct ggtctggcaa tgccccatgc
    32461 gccctattct tcgattattg gtatcccact ggtggaaccc gttatacgtc ggacattggg
    32521 gattattcgc cgcaaagatg ccgtgctttc tccggcagct gaacgctttt ttgccttgct
    32581 gattaatttg tggactgacg ataaagacaa tttgtggacc aatatcgtcg aacgccagag
    32641 acatgcgtta caggagatag gttaatacac cggcgcagga gagactgcgc cggttaaatc
    32701 agcggttagt gtgcgttaac caccacccac attggtccct gcccgaccgc ataacggcct
    32761 ttttcatgca gtagtccctg ctcgccaaca atttcgtata ccgagatgtg gtgagatttt
    32821 tgcccggcgg caatcagata cttgccgctg tgatcaacat tgaagccgcg cggctgggtt
    32881 tccgttggct ggaagccttc tttactcaac acgctgccat cttccgaaac gctgaaaacg
    32941 gtaatcaggc tggcggtacg gtcgcaggcg tataaatggc gaccatccgg ggtgatatga
    33001 atatcagccg cccaacgggt gtcggagaag ttttccggca tcatatccag cgtctggaca
    33061 cattcgatat taccgtgcgg atctttcagt tcccagacat ccactgagct gtttaactca
    33121 ttgacgcaat acgcatattg ttcgtttgga tggaatacca tatgacgcgg gccggcccct
    33181 tcaacggtgg tcacttccgc agggtcctgc gccacgagat gaccatcatc gctgaccgta
    33241 aacaggcaaa tgcgatcctg ctttaatgcc ggaacccaca gcgtacggtt gtccggtgag
    33301 atattggcgg aatggcaacc gtccagcccc tcgaccacat cgacgacgcc cactggcagg
    33361 ccatcttcca gacgcgttac gctcacgtta cccgcattgt aagaacctac aaagacaaac
    33421 tgcccctggt gatcggtgga aatatgcgtc ggactacccg gcagcgcaga ctctgcggca
    33481 aaggtcagtg cgccatcgtc cggggcgata cgatacgcca ggacgcgaaa ctcagggcga
    33541 acaccaacat agagataacg tttgtccggg ctgaccacca tcggctgcac ctgccccggc
    33601 acatcgacaa cctgtgtcag cgtcagtgcg ccttcatgat tcaggttcca gacgtgaatt
    33661 tgctggctct cagggctggc gatataaact gtttgcttca tgaatgctcc tttgcattta
    33721 gcaactgact gtaacagcta aaattagtcg cttttggcgg tgaatgctga attaaaacgc
    33781 agaattttgt ccgatagtgg tatcggtgta ccattcgcgc agacgaaaat tcttaacctt
    33841 aatctggaat acgccatgac cacacgcgtg attgctctcg acttagacgg caccttattg
    33901 accccgaaaa agaccctgct tccttcatcg atagaagccc tggcccgcgc tcgcgaagca
    33961 ggctatcaat taatcatcgt cacaggtcgc catcacgtcg ctattcatcc tttttatcag
    34021 gcgctggcgc tggatacacc tgctatttgc tgtaatggca cctatttgta tgattatcat
    34081 gcaaaaaccg tgctggaagc ggacccaatg cccgttaata aagccctgca actcattgag
    34141 atgctgaatg aacaccacat tcacggtctg atgtatgtgg atgatgcaat ggtctatgag
    34201 cacccgaccg ggcatgtcat tcgcacgtct aactgggcgc aaaccctgcc gccggaacag
    34261 cgtccgactt tcacacaagt cgcttctctg gctgaaacgg cgcaacaagt taacgccgta
    34321 tggaagttcg ccctgacgca cgatgacctg ccgcaattgc agcattttgg taagcatgtc
    34381 gaacatgaac tgggactgga gtgtgaatgg tcctggcacg atcaggttga tattgcacgc
    34441 ggcggcaaca gcaaaggtaa acgtttgacg aaatgggttg aggcgcaagg ctggtcgatg
    34501 gaaaacgtcg tggcgttcgg cgataacttt aatgatatca gtatgctgga ggccgctggt
    34561 acaggcgtgg cgatgggcaa cgccgatgac gcggtaaaag cgcgcgccaa cattgtgatt
    34621 ggtgataaca ccaccgacag cattgcccag ttcatttata gccacctgat ttaatcaggc
    34681 ggttatcgac acacttttaa tttgcgcgta cagccacagg ccaggtttga tcaccagttc
    34741 atccctggcc cacgggctga tacgcgccca cagcgtttta cctccgactt ccagttccac
    34801 ttccacctgg ccgttgtcgt cataactatt aacaactttt gcccgcaata cgttacgaat
    34861 gctggtttgc tgcggcggtt gtaaaaccag agaaacatcg gaagcctgaa tgcggatacg
    34921 tagcgcagct tgcagcggtt cgtccagctt attgacccac aaatgctgat cgcccagcgc
    34981 cagcgcagtc atcgcgtaat gcggatgatg ctcaagtacc gtcactttca gaatgctact
    35041 ttgttgctct ttcggcagcc acggattcat cacgctacta ccccagactt cttccagtgc
    35101 gccaaaggct ttcacctgac cgttttccag taccatcact ctgtctgcca gatggaggat
    35161 ctcatccagc gaatggctga catacaacat cgggatgttg atttcccgcg tcagacgttg
    35221 cagataaggc aacagttcgc gtttacgcgg aatatccagt gacgccagcg gttcatccag
    35281 caacagcaat tctggtgccg tcagcaaagc ccgcccaatc gctacgcgct gtttttcgcc
    35341 tccggacagg ctgcctggta aacggtcaag caacggttca atgcctaaaa gcgccaccag
    35401 cttatcgaac tgatcgacca tgcttttcgc catgccgtag cgcagattgc cacgcacttt
    35461 gtaatgcgga aacagccgcg catcctgaaa aacatagcca acgcgacgct tttctggcgt
    35521 caggcagata cctttttcgg catcatttag tacccgacca ttgaggacaa tcctcccttt
    35581 ttgcgggcgc gtcagtccac tgatggcgtt aatcagcgaa gtttttccgg caccggagac
    35641 gccaaagata gcagtgatgc cattggcggg cagcgtttca ttaatggtca ggcaatggtt
    35701 gcccaaggtc tgggaaaaat tcagttccag catgattagc gccccgcccg ttcacggctg
    35761 attctggcca gccattctga aatcaacagg gagatcatcg ccagcgcaat agaaataatg
    35821 cacagtctcg ccgcgccact ttccccgccg ggggtctgga tcagggtata catggcagaa
    35881 ggaatggttc gcgtttcacc aggaatgttc gacacaaagg tgatggttgc gccaaattca
    35941 ccgagagaac gagcaaaagc cagtaccgta ccaacaataa ttcccggtaa ggtcagcggt
    36001 atcgtgatag taaagaaaac gcgccagcgc ccggccccca gtgttcttgc ggcctgttcc
    36061 agtttgacat caaccccttc cagcgccaga cgaattgccc gcaccatcag cggaaacgac
    36121 atgacggcgg cagcgagaac cgcgccgcgc cagctaaagg cgaaggtaat gccaaaccag
    36181 tcatacagac gttcaccgat aaacccgcgc cgtcccatcg aaactaataa taagtaaccg
    36241 acgaccacgg gcggtaacac cagcggtaga tgcagtacgc tgtcgagcag agctttgccc
    36301 ggaaacgtgc aacgcaccag taaccaggca aaaaagatcc caaacggcag gctaaacagc
    36361 acagccaggg aagaaacttt caggcttaat aaaactgcct gccattctgg atcggtcagt
    36421 atcattactt ggttgtaaat ccgtaacgtt taaagatttc cgcagcctgc ggccctttca
    36481 aataatcata aaatgctttc actgtcgcat tgttatgccc ttccacaaca gcaaccgggt
    36541 attccacttt tttatgcgaa tcttccggga aggtggcgac cacttttacc cctttgctgg
    36601 caactgcgtc agaaccgtaa actatgccca gcggcgcttc attgcgttcg accaacgcca
    36661 gcgcgccacg aacatcttcc gccggagcca gtttcggaga gagcgtatcc catgcgccca
    36721 gtttttgcag tgcttctttt gcataaatgc cagcgggaac atgttccgga tcgccaaccg
    36781 ccagacgacc gccattcagc agcgaagtcc agttggtttt gctgtcgatg gtgaaatctt
    36841 tctgctcgct ggctttcggt gctacaacga ccaggctatt gccgagcagt gtctgacgcg
    36901 tagcagtatc gatcgctttt ttatcaactg cataatccat ccatttctga tcggcagaaa
    36961 taaacagatc cgcaggcgca cccgcttcaa tctgacgggc gagagtagat gacgaagcga
    37021 aagaagaaac cacatccacg cctttttctt ttttatactg cgtagcaatg tcctgcattg
    37081 cgttagtcag tgatgcggcg gcgaacaccg tgattttccc ttcatctgcc agtgtattgc
    37141 cagcaacagc gaaagagagt gccgccccgg caaacaagtt caaccattta cgagccattg
    37201 gtaacccctt aatgtaacgt tatataggtg acaatataac ggctagctca gaattttccc
    37261 agcagttatt gctaaccttt gtgaggtaga taagaaaaaa tatcggcagg aaaagcagga
    37321 agttgagaaa aagaaaatgc ccgactaagc gggcattcag ggaatcaatg attttgtccg
    37381 ggctggtctt ttttaccaac accagaaaag atgttgaata cttcaccaag accgtaaatc
    37441 agacccagga tgatggccat cacgacaggt accatgatta cggcgaatac cagacttttt
    37501 aataactcta acatggtcaa ctccagatat agtcatgaga ctattctaac cgctaagcac
    37561 agaaaagcac tccccttttg tgcggtcagc tttgcgtggc gttcgttttc cgtcacaata
    37621 agacttttgc caggacattg ttatgcaggc cgaaatcctt ctcaccctta agctccaaca
    37681 aaaattattc gccgacccgc gccggatttc gctactaaaa cacattgcgc tttccggttc
    37741 cattagccag ggagcgaaag atgccggtat cagctataaa agcgcctggg atgccattaa
    37801 cgagatgaat cagttaagtg agcatattct ggtcgagcgc gcaacaggcg gtaaaggtgg
    37861 cggcggcgca gtactgaccc gctatggtca gcgactgatt cagctctatg acttactggc
    37921 gcaaatccag caaaaagcct ttgatgtgtt aagtgacgat gacgccctgc cgctgaacag
    37981 cctgctggcc gcgatctcac gtttttcact gcaaaccagc gcccgtaacc agtggttcgg
    38041 caccatcacc gcccgcgatc atgatgacgt tcaacagcat gttgatgtct tactggctga
    38101 cggaaaaaca cgcctgaaag tcgcaattac cgcacaaagc ggcgcgcgtc tggggctgga
    38161 tgaaggcaaa gaagtgttga tattgctaaa agcgccgtgg gtaggtatta ctcaggacga
    38221 ggcggtcgcg caaaacgctg acaaccaatt accgggtatt attagtcata ttgagcgcgg
    38281 cgcagagcag tgcgaagtat taatggcgct acccgacggg caaacactgt gcgccacagt
    38341 gccggtaaat gaagcgactt ctcttcagca agggcagaat gtcacggcct actttaatgc
    38401 cgacagcgtg attatcgcca cgctgtgcta agcgtgttga caatttgtta tgaaacacgt
    38461 atccctgtca gtaatcgctg cacaaaatgg gatataaaat gtcatcgttg caaattttgc
    38521 aaggcacgtt tcgtcttagc gacacaaaaa cgctgcaatt gcctcagcta acgttaaacg
    38581 cgggtgatag ttgggcgttt gtcggttcga atggaagcgg gaaatcggcc ctggcccgcg
    38641 cgctggcggg ggaacttccg cttttgaaag gtgaacggca aagccagttt tcccacatca
    38701 ctcgtctctc cttcgagcaa ttgcaaaaac tcgtcagcga cgaatggcag cggaataaca
    38761 ccgatatgct cggccctggc gaagatgaca ccggacgcac tacggctgag atcattcagg
    38821 atgaagtaaa ggatgcaccg cgttgcatgc aactggcgca gcagttcggt attaccgccc
    38881 tcctcgaccg acgctttaaa tacctttcca ctggcgagac gcgaaaaagc ctgctgtgtc
    38941 aggcgctgat gtcggagcct gacttgttga ttcttgatga gccgttcgat ggcctggatg
    39001 ttgcctcacg tcagcagctg gctgagcgac tcgcctcgtt acatcagtcc ggtattactc
    39061 tggtactggt gctcaatcgc ttcgatgaga tcccggagtt tgtccagttt gctggcgtgc
    39121 tggcggattg cacgttagcg gaaactggcg ctaaagagga actgctccaa caagcactcg
    39181 tcgcgcaact ggcgcatagt gaacagcttg aaggtgtgca actgccggag ccggatgaac
    39241 cttcagcacg tcacgcctta cccgccaacg aaccgcgcat tgtgctgaac aatggcgtgg
    39301 tttcttataa cgatcgcccc attcttaata accttagctg gcaggtgaat ccaggcgaac
    39361 actggcaaat tgtcgggcca aatggtgcag gaaaatcgac gttattaagc ctggttactg
    39421 gcgatcatcc gcaaggttac agcaacgatt tgacgctttt cggacgacgt cgcggcagcg
    39481 gcgaaaccat ctgggatatc aaaaagcata tcggttacgt cagcagtagt ttgcatctgg
    39541 attaccgggt cagtactact gtgcgtaatg tgattctttc tggctatttt gattcgattg
    39601 gcatttatca ggccgtttcg gatcgccagc aaaaactggt gcagcagtgg ctggatattc
    39661 tcggcattga taaacgcacg gctgacgctc ctttccatag tctttcctgg ggacagcagc
    39721 gtctggcgct gatcgtccgc gcactggtga aacatccgac gttgcttatt ctcgatgaac
    39781 cactacaggg gcttgatccg ctcaatcgcc agcttatccg ccgttttgtt gatgtgctga
    39841 ttagcgaagg tgaaacgcaa ttgttgtttg tttcgcacca cgctgaagat gcgcctgcct
    39901 gtattaccca tcgccttgag ttcgtgccgg acggtggact ctatcgctat gtgctgacaa
    39961 aaatatatta agtcggtagt gctgaccttg ccggaggcgg cctaagcgcc ctctccggcc
    40021 aacggttcga cgcatgcagg catgaaaccg cttctttttt cagataaaaa gcgcaatcat
    40081 tcataaaccc tctgttttat aatcacttaa tcgcgcataa aaaacggcta aattcttgtg
    40141 taaacgattc cactaattta ttccatgtca cacttttcgc atctttgtta tgctatggtt
    40201 atttcatacc ataagcctaa tggagcgaat tatgagagtt ctggttaccg gtggtagcgg
    40261 ttacattgga agtcatacct gcgtgcaatt actgcaaaac ggtcatgatg tcatcattct
    40321 tgataacctc tgtaacagta agcgcagcgt actgcctgtt atcgagcgtt taggcggcaa
    40381 acatccaacg tttgttgaag gcgatattcg taacgaagcg ttgatgaccg agatactgca
    40441 cgatcacgct atcgacaccg tgatccactt cgccgggctg aaagccgtcg gcgaatcggt
    40501 acaaaaaccg ctggaatatt acgacaacaa tgtcaacggc actctgcgcc tgattagcgc
    40561 catgcgcgcc gctaacgtca aaaactttat ttttagctcc tccgctaccg tttatggcga
    40621 tcagcccaaa attccatacg ttgaaagctt cccgaccggc acaccgcaaa gcccttatgg
    40681 caaaagcaaa ttgatggtgg aacagatcct caccgacctg caaaaagccc agccggactg
    40741 gagcattgcc ctgctgcgct acttcaaccc ggttggcgcg catccgtcgg gcgatatggg
    40801 cgaagatccg caaggcattc cgaataacct gatgccatac atcgcccagg ttgctgtagg
    40861 ccgtcgcgac tcgctggcga tttttggtaa cgattatccg accgaagatg gtactggcgt
    40921 acgcgattac atccacgtaa tggatctggc ggacggtcac gtcgtggcga tggaaaaact
    40981 ggcgaacaag ccaggcgtac acatctacaa cctcggtgct ggcgtaggca gcagcgtgct
    41041 ggacgtggtt aatgccttca gcaaagcctg cggcaaaccg gttaactatc attttgcacc
    41101 gcgtcgcgaa ggcgaccttc cggcctactg ggcggacgcc agcaaagccg accgtgaact
    41161 gaactggcgc gtaacgcgca cactcgatga aatggcgcag gacacctggc actggcagtc
    41221 acgccatcca cagggatatc ccgattaagg aacgaccatg acgcaattta atcccgttga
    41281 tcatccacat cgccgctaca acccgctcac cgggcaatgg attctggttt caccgcaccg
    41341 cgctaagcgc ccctggcagg gggcgcagga aacgccagcc aaacaggtgt tacctgcgca
    41401 cgatccagat tgcttcctct gcgcaggtaa tgtgcgggtg acaggcgata aaaaccccga
    41461 ttacaccggg acttacgttt tcactaatga ctttgcggct ttgatgtctg acacgccaga
    41521 tgcgccagaa agtcacgatc ctttgatgcg ttgccagagc gcgcgcggca ccagccgggt
    41581 gatctgcttt tcaccggatc acagtaaaac gctgccagag ctcagcgttg cagcattgac
    41641 ggaaatcgtc aaaacctggc aggagcaaac cgcagaactg gggaaaacgt acccatgggt
    41701 gcaggttttt gaaaacaaag gcgctgcgat gggctgctct aacccgcatc cgcacggtca
    41761 gatttgggca aatagcttcc tgcctaacga agctgagcgc gaagaccgcc tgcaaaaaga
    41821 atattttgcc gaacagaaat caccaatgct ggtggattat gttcagcgcg agctggcaga
    41881 cggtagccgt accgttgtcg aaaccgaaca ctggttagcc gtcgtgcctt actgggctgc
    41941 ctggccgttc gaaacgctac tgctgcccaa agcccacgtt ttgcggatca ccgatttgac
    42001 cgacgcccag cgcagcgatt tggcgctggc gttgaaaaag ctgaccagtc gttatgacaa
    42061 cctcttccag tgctccttcc cctactctat gggctggcac ggcgcaccat ttaatggcga
    42121 agagaatcaa cactggcagc tgcacgcgca cttttatccg cctctgctgc gctccgccac
    42181 cgtacgtaaa tttatggttg gttatgaaat gctggcagag acccagcgag acctgaccgc
    42241 agaacaggca gcagagcgtt tgcgcgcagt cagcgatatc cattttcgcg aatccggagt
    42301 gtaagaaatg agtctgaaag aaaaaacaca atctctgttt gccaacgcat ttggctaccc
    42361 tgccactcac accattcagg cgcctggccg cgtgaatttg attggtgaac acaccgacta
    42421 caacgacggt ttcgttctgc cctgcgcgat tgattatcaa accgtgatca gctgtgcgcc
    42481 acgcgatgac cgtaaagttc gcgtaatggc agccgattat gaaaatcagc tcgacgagtt
    42541 ttccctcgat gcgcccattg tcgcgcatga aaactatcaa tgggcgaact acgttcgtgg
    42601 cgtggtgaaa catctgcaac tgcgtaacaa cagcttcggc ggcgtggaca tggtgatcag
    42661 cggcaatgtg ccgcagggtg ccgggttaag ttcttccgct tcactggaag tcgcggtcgg
    42721 aaccgtattg cagcagcttt atcatctgcc gctggacggc gcacaaatcg cgcttaacgg
    42781 tcaggaagca gaaaaccagt ttgttggctg taactgcggg atcatggatc agctaatttc
    42841 cgcactcggc aagaaagatc atgccttgct gattgactgc cgctcactgg ggactaaagc
    42901 agtttccatg ccgaaaggtg tggctgtcgt catcatcaac agtaacttca aacgtaccct
    42961 ggttggcagc gaatacaaca cccgtcgtga acagtgcgaa accggtgcgc gtttcttcca
    43021 gcagccagct ctgcgcgatg tcaccattga agagttcaac gctgttgcac atgagctgga
    43081 cccaatcgtg gcgaaacgcg tgcggcatat cctgactgaa aacgcccgca ccgttgaagc
    43141 tgccagcgcg ctggagcaag gcgacctgaa acgtatgggc gagttgatgg cggaatctca
    43201 tgcctctatg cgcgatgatt tcgaaatcac cgtgccgcaa attgacactc tggtagaaat
    43261 cgtcaaagct gtgattggcg acaaaggtgg cgtacgcatg accggcggcg gatttggcgg
    43321 ctgtatcgtc gcgctgatcc cggaagagct ggtgcctgcc gtacagcaag ctgtcgctga
    43381 acaatatgaa gcaaaaacag gtattaaaga gactttttac gtttgtaaac catcacaagg
    43441 agcaggacag tgctgaacga aactcccgca ctggcacccg atggtcagcc gtaccgactg
    43501 ttaactttgc gtaacaacgc agggatggta gtcacgctga tggactgggg tgcgacttta
    43561 ctttcagctc gtattccgct ttccgatggc agcgtccgcg aggcgctgct cggctgtgcc
    43621 agcccggaat gctatcagga tcaggctgcg tttctggggg cctctattgg tcgttatgcc
    43681 aaccgcatcg ccaatagccg ttataccttt gacggtgaaa ccgtgacgct ttcgccaagt
    43741 cagggcgtta accagctgca cggcggcccg gaagggttcg acaaacgtcg ctggcagatt
    43801 gtgaaccaga acgatcgtca ggtgctgttt gccctgagtt cagatgatgg cgatcagggc
    43861 ttcccgggta atctcggcgc gacggtgcaa tatcgtctga ccgacgataa ccgtatctct
    43921 attacttatc gcgccacagt tgataaacct tgcccggtga atatgactaa tcacgtctat
    43981 ttcaatcttg acggcgagca gtctgacgtg cgcaatcaca agttgcagat tctggcggac
    44041 gaatatctgc cggttgatga aggcggcatt ccgcacgacg gcctgaaatc tgtcgccgga
    44101 acgtcttttg atttccgtag cgccaaaatc atcgccagtg agtttcttgc cgacgacgat
    44161 cagcgcaaag tgaaaggtta cgatcacgca ttcttgttac aggccaaagg cgatggcaag
    44221 aaagtggcgg cgcatgtctg gtcagcagat gaaaaattgc agctgaaggt ctacaccacc
    44281 gctccggctc tgcaattcta ctccggcaac ttcctcggcg gcacgccatc aagaggaact
    44341 gaaccttacg ccgactggca aggcctggca ctggaaagcg agtttctgcc ggacagccca
    44401 aaccaccctg aatggccgca accggactgc ttcctgcgtc ctggcgaaga gtattccagc
    44461 ctgacggaat atcagtttat tgctgagtag cacctgaagc cctcgtaatg agggctttat
    44521 ccttatattg ctcaaatcac cagcaaacac cgacatattt gcaactcaat attcacaaca
    44581 accttacact gcgacactat tttcgctatg gttatgcgta agcattgctg ttgcttcgtc
    44641 acggcaatat aatgagaatt attatcatta aaaaatgatt tgaggagtaa gtatatggct
    44701 gtaactaagc tggttctggt tcgtcatggc gaaagtcagt ggaacaaaga aaaccgtttc
    44761 accggttggt acgacgtgga tctgtctgag aaaggcgtaa gcgaagcaaa agcagcaggt
    44821 aagctgctga aagaggaagg ttacagcttt gactttgctt acacctctgt gctgaaacgc
    44881 gctatccata ccctgtggaa tgtgctggac gaactggatc aggcatggct gcccgttgag
    44941 aaatcctgga aactgaacga acgtcactac ggtgcgttgc agggtctgaa caaagcggaa
    45001 actgctgaaa agtatggcga cgagcaggtg aaacagtggc gtcgtggttt tgcagtgact
    45061 ccgccggaac tgactaaaga tgatgagcgt tatccgggtc acgatccgcg ttacgcgaaa
    45121 ctgagcgaaa aagaactgcc gctgacggaa agcctggcgc tgaccattga ccgcgtgatc
    45181 ccttactgga atgaaactat tctgccgcgt atgaagagcg gtgagcgcgt gatcatcgct
    45241 gcacacggta actctttacg tgcgctggtg aaatatcttg ataacatgag cgaagaagag
    45301 attcttgagc ttaatatccc gactggcgtg ccgctggtgt atgagttcga cgagaatttc
    45361 aaaccgctga aacgctatta tctgggtaat gctgacgaga tcgcagcgaa agcagcggcg
    45421 gttgcaaacc agggtaaagc gaagtaaacg tcattcgttt aaaatgagaa agccgactgc
    45481 aagtgagtcg gcatttttgt tgctaacaat ggagcacatt gcctgatgcg acgctgcgcg
    45541 acttatcagg cctgtggtga ttcatcggat acgccactct gacggcgcat ccgacaatta
    45601 aaccttaccc gcgacgcgct tttactgcat tcgccagttg acgtaacaga gcatcggtat
    45661 cttcccagcc gatgcaggca tcggtgatgc tcttaccgta ggccagcggc tccccgctct
    45721 cgaggctctg attgccttcc accagatggc tttccaccat cacgccaata atggcctttt
    45781 cgccaccggc aatctgctgg caaacgtcag cacaaacatc catctgcttt ttgaattgtt
    45841 tggacgagtt agcatggctg aaatcgatca tcacctgtgc tggcaggcct gctttgttca
    45901 gcccttcttt cacttcagca acgtgcttcg cgctgtagtt aggctcttta ccgccgcgca
    45961 gaatgatatg gcaatcgccg ttaccgctgg tattcacaat cgccgaatgc ccccatttcg
    46021 ttacggacag gaagcagtgc ggcgcaccgg cggcattaat ggcatcgata gccactttaa
    46081 tcgtaccgtc ggtgccattt ttgaagccga ccggacaaga aagccctgat gccagttcgc
    46141 ggtgcacctg cgattcggtg gtacgtgcgc caattgcgcc ccagctcatc aggtcagcga
    46201 gatattgtgg ggtgatcata tcgagaaact cacctgccgc tggcagaccg ctgtcgttaa
    46261 tatcaagcag caatttacgg gctatacgca gaccgtcgtt gatctggaag ctattatcca
    46321 tatgcggatc gttaatcagc cctttccagc ccaccgtggt acgcggcttt tcaaaataga
    46381 cgcgcattac gatttccagc tcatctttca gctcttcacg cagcgccagc aagcgagtgg
    46441 catactcttt tgccgcgaca ggatcatgaa ttgagcatgg gccaatcaca accaacaggc
    46501 gatcatcatt acctttcagg atcttatgga tcgcttttcg ggcatgggca accgtattcg
    46561 cggcattttc agtagcgggg aatttttcca gcaatgcgac aggaggaagt aactctttga
    46621 tttctttgat gcgtaaatcg tcgttctgat aattcatgtc tgttccagtg ttgccatact
    46681 tatcttagtg aatgcaatac ttccaatcta tatcttccgt cagaatgtgt aaacggggtt
    46741 ttacactatg aacggattaa tcctggaatt agcaagaaaa acgccagatt gtcgcgaaaa
    46801 acgagatctc tcctacaatt tctaactgta actcctttgt ttgttaatta tttcaagatt
    46861 ctctgctgcg tttcataacc tggctgaaat cttaaaccaa tgccttatat tcacctgcaa
    46921 atgcactgtt ggaagaggtt atccgacata acgaccataa caggagcatc ctatgaaaat
    46981 gacaaaactg gccacattat ttctgactgc cactctaagc cttgccagcg gtgccgcact
    47041 ggctgccgat agcggagcgc aaactaataa cggccaggca aacgccgcag ctgatgcggg
    47101 ccaggtagcc cctgacgccc gtgaaaatgt cgcgccaaat aacgtcgaca ataacggggt
    47161 aaataccggt tctggtggca caatgctgca ttcggatggt tcttcaatga acaatgacgg
    47221 aatgaccaaa gatgaagagc acaagaacac catgtgcaaa gatggtcgct gtccggatat
    47281 caataaaaag gtacaaaccg gtgatggcat caataatgat gtcgatacca aaaccgacgg
    47341 taccacgcag taaccctgca aaatagccaa taatgggaga gcggagtctc tcccttttaa
    47401 tttttcactt ctaaaagcag tatgatgtca taaagttaag tgagaataat taagggatga
    47461 ctcgtcatgg cgcactcaca ctcacacacg tcctcacacc tgccagaaga taataatgct
    47521 cgtcgcttgt tgtatgcttt cggcgtgact gccgggttta tgctggtaga agtcgttggt
    47581 ggttttcttt ctggttctct ggcattgctg gccgatgcgg gtcatatgtt gaccgatact
    47641 gccgccctgc ttttcgccct actcgccgtg caattttcac gtcgtcctcc caccattcgc
    47701 cacactttcg gttggctaag actgaccacg ctcgcggcgt ttgtgaatgc catagccctc
    47761 gtggtaatta ccattttgat tgtctgggag gcgatagaac gcttccgcac gccgcgtccg
    47821 gtcgagggcg gcatgatgat ggcaattgcc gtggctgggt tgctggcaaa tatactttct
    47881 ttctggttac ttcatcacgg cagcgaagag aaaaacctca acgtacgagc agcggcactg
    47941 catgtgctcg gggacctgct gggttcggtt ggggctatta ttgccgcgtt gattattatc
    48001 tggactggct ggacacctgc tgaccccatt ctctcgatac tggtgtcgct tctggttctg
    48061 cgtagcgcgt ggcgactgtt gaaagatagc gtgaatgaat tacttgaagg tgcaccggta
    48121 tcgctggata tcgctgaact gaagcgtcgt atgtgccggg aaatcccgga agtccgcaat
    48181 gtgcaccatg tacatgtatg gatggtaggc gagaatccgg tgatgacgtt gcatgtgcag
    48241 gtgatcccgc cacacgatca cgatgccttg ttggatcaga tccaacacta cctgatggat
    48301 cactatcaga ttgagcacgc caccattcag atggaatatc aaccttgtca tgggccagac
    48361 tgccacctga atgaaggggt gtctggccat tcacatcatc accattaatg ggacagcgcg
    48421 cgtgagcctc tttcacgtgc gctgttgatc cacatccggc tgccgttgag cgcaataaag
    48481 gtcaggatga tgtactccag agacatggcg taaacgccct gaagtgcaaa aataacgacg
    48541 ctaatcacgt taataatcac ccacaacagc cagttttcca catacttacg cgtcatcaga
    48601 atcattgcca cgatagataa caccatcatg catgaatccc agaacgggaa agcgtccggt
    48661 tgcagttcag gcatcacaac ctgtaatcct aatgcttgca tgatcatgac tgccacgcgg
    48721 gtcaaaaatg caaacaccgg attgataaat accgtcatca gaccaatcga aacaacgcaa
    48781 accgccaacc agctgagtgc cttcggcaat ggcaaccagc gaattttcaa ctccgcctcg
    48841 ttctgactgg tttgtcgcga ccacgcatac caaccgtaaa tattcgcggc aaagaaaaac
    48901 acctgtaata gcaggctggc atacagctga atctgaaaga aaataatgcc aaacaaggtg
    48961 acgttgatca ggccaaagaa gtagttgctg atcttctcca gactggcaag gccaatacac
    49021 agcaacccgg cgatcgtgcc taccgcttcg atccatgaga gatcataacc gcctgcccct
    49081 attggtatat gtaccaggat attctgcaca ctaaaaaaat ccatctttcc cccaaagcgt
    49141 aacgttacgc cttattattc gttatccacg tagtgtagcc gcaaaatcca gcatacgatt
    49201 gagcggcacc agcgccctct ctcgcagcct ttcatcaaca tgaacctcgt gattgcttcc
    49261 ttcctgttct aatgcctctg cgatggactg aaggccattc atggccatcc acggacaatg
    49321 cgcgcagctg cggcaggttg caccctcacc tgcggttggt gcttccagta actctttatc
    49381 tggcactgcc tgctgcattt tgtagaaaat gccccgatcg gttgccacga taagccgctg
    49441 atgtggcaat gttttcgcag cagcgatcag ttgactggtg gaaccgaccg catccgccat
    49501 atcgacaata gcttgtggtg attctggatg caccagtatg gcagcatccg ggtattcttc
    49561 ttgcaagcgg gttaacgcct gagtcttaaa ttcatcatgc acaatacagg caccctgcca
    49621 gcatagaatg tctccacccg tctgtttttg cacgtaacgc cccagatgtt tgtcgggtgc
    49681 ccagatgatt ttttcaccca aactatcaag atgatcaata agttcgacgg caatgcttga
    49741 agttaccacc caatctgcgc gcgcttttac cgcagcagaa gtgttggcgt agacgacgac
    49801 agtacgatcg ggatgggcat cgcaaaatgc gttaaattct tcaacagggc agccgagatc
    49861 cagtgaacat tcagcctgaa gtgtcggcat cagaattgtt ttttccggac tgagaatttt
    49921 ggcggtttct cccataaatc tcaccccagc gactaacaaa gtagaagcgg gatgctttgc
    49981 accgaagcgc gccatttcca gagaatcaga aatacagcca ccggtttctt ctgccagttg
    50041 ttgaatttcg ggatcggtat agtagtgggc aaccatcacc gcattacgtt cttttagcag
    50101 acgttttatc ttctcgcggt aatacgcttt ttcatcaatg cttaacggcg tcggcttcgg
    50161 ggggaaagga taaatcgccg tgtctggatc aaacattacg ctcatcttac catctcgttt
    50221 tacaggctta acgttaaaac cgacattagc gtaatattcg ctgttatcgg tgtaatttgt
    50281 tttatatgct aaacaagata gtcgaatggt ggggaaaagt cactcgaatt acgcctaatt
    50341 ccaccgacgt agagttgctt gcacagtaag ctctgacgat gtcatcaggt ttacaaagtg
    50401 aaattgagga tgcctgaata gcaaaaaagc gcctttaggg cgctttttta cattggtggg
    50461 tcgtgcagga tgactcgctt cgctcgccct tcgggccgtc gccgcgagcg gctccgttgt
    50521 ttcgctgcgc tcaactcaaa cctgctgcag gttcgaatct ttgagtatcg cagatagcaa
    50581 aaaagcgcct ttagggcgct tttttacatt ggtgggtcgt gcaggattcg aacctgcgac
    50641 caattgatta aaagtcaact gctctaccaa ctgagctaac gaccccttgc gggattatgc
    50701 tgctttaatc attcaggatg gtgggtcgtg caggattcga acctgcgacc aattgattaa
    50761 aagtcaactg ctctaccaac tgagctaacg acccatacgg gtgttacctg aaagatttct
    50821 actcggggcc aatctttaag attggtgtgt cgtgcaggat gactcgcttc gcttgcccta
    50881 cgggccgttg ctggcgcaac gttatccttc acgcttaaca tctgagtttg atgttaaaat
    50941 tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac tgctctacca
    51001 actgagctaa cgacccgagt ggtgggtgat gacgggatcg aaccgccgac cccctccttg
    51061 taagggaggt gctctcccag ctgagctaat cacccgatac tgcgctggat actacaaact
    51121 acttccaccg aacccaccgt aattagtggt gggtcgtgca ggatgactcg cttcgctcgc
    51181 cctacgggcc gttgctgacg caacgttatc ctacacgctt aacatctgtc ttcgatgcta
    51241 aaactggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct
    51301 accaactgag ctaacgaccc ggtggtgggt gatgacggga tcgaaccgcc gaccccctcc
    51361 ttgtaaggga ggtgctctcc cagctgagct aatcacccaa tactacgctg gatactacaa
    51421 ctaactatgt tagtggtggg tcgtgcagga tgactcgact tcgtctcgcc cttcgggccg
    51481 ttgctgatgc aacgttatcc ttcacgtttt actaccgctt ccaccttagc gattggtggg
    51541 tcgtgcagga ttcgaacctg cgaccaattg attaaaagtc aactgctcta ccaactgagc
    51601 taacgaccca ctttttcgtt gctttcggtt tgtttgatat cccgtggcaa cggcggcata
    51661 tattactgat ttcagacttg agcgcaacaa aaatttcgat gcagatcact caactgctta
    51721 tgattcgcac gacacgacca gaaataatgc gacttctggt cgtgtgttat gcattacatc
    51781 gcgttcagac gtttttgtgc ctgtttagcg ccatcagtac cagggtattt actgataacc
    51841 tgctggtata cggctttcgc ttttgcggtg tcacctttgt cctgcatgat gacgccgact
    51901 ttaaacatcg catcagcagc ctttggtgac ttcggatagt ttttcactac cgaagcaaaa
    51961 tagtacgccg catcatcttt tttacccttg ttgtagttta actgacccag ccaataattg
    52021 gcgtttggca ggtaagttga atcagggtaa tttttgatga aattctgaaa tgccaccatt
    52081 gcgtcatcct ggcgggattt atcctgcacc agcgcaatag ctgcattgta atccgtgttt
    52141 gcatcgccgc ttttcaccgg cgcgccagca ttcgcagtac cagcatcagc tgtcggcgtc
    52201 gttgatgccg ccgcaccgct ttgatcgctg ctggttgatt gcgccgctgc accaccgctg
    52261 ctgaggctgt cgatctgcaa caggatctgc ttttgccgct ccacgacctg attcagttga
    52321 tactgatttt cctgaatctg accacgcagg gaatcaatat cggattgatt atcagaaagt
    52381 tgttgctgga gttgggttaa aagctggctg tgagcattag aaatacgctc aagttgagtg
    52441 acgcggtctt cgaccgagcc tgagccgaca ctactgattg gtgcctgagc aaaagcggcc
    52501 cagggggccg ctataccaac cagtaacgac agactcaata gttgatgtct gaagttactg
    52561 ctcatgcaat tctcttagta aaccagtacc gcacgacggt ttttggagta tgccgcttcg
    52621 tcatgaccca gtactgcagg tttttcttta ccgtaagaaa cgatggagat ctggtctgca
    52681 gaaacgcctt taccctgcag gtacatctta acggcgttcg cacgacgttc acccagggag
    52741 atgttgtatt ccggagtacc acgttcgtcc gcgtgacctt ctacggtgac tttgtaagac
    52801 gggttgctac gcaggaagtt tgcatgtgca tccagcattt gagcgaagtc agaacggata
    52861 tcgtacttgt ccagatcgaa gtaaacgatg ttgttctgct gcagctgttg catttgcaga
    52921 cgagcctgct cttcggaaga catgttgccg ttgccgccgt tcgcatccat accagtgccg
    52981 gcacccagca tgccttcgct gccgtcattg ctggcgttct tgttggaaga acatgccgca
    53041 attgccataa caggcagagc aatcatcagc cctttcagca ctttgttcag ttgcatttca
    53101 atgattcctt tactattcaa ttaattatta tcacagatac ggcgaccagg cagggaattt
    53161 gacctgtcca tcagttgccg gaagacgcgc tttgaaacgc ccatctgtag aaaccaaatt
    53221 cagcacggat cccatcccct gagaagagct gtagattacc atagtgccgt taggtgccag
    53281 acttggcgtt tcatccagga acgtggacga cagaacttgt acgcctcccg ttgccagatc
    53341 ttgtttggca atgtgctgct gcccaccatt ggagctgacc attaccataa atttaccgtc
    53401 gctgctgaca tccgcatcct ggttctgcga accttcccag gtaatacgtt gtggcgcacc
    53461 gccgttgata ttcactttat aaacctgcgg acgaccggcc tggtcagaag taaatgccag
    53521 gttctggcta tccgggaacc aggtcggttc ggtattgtta ctgcgaccat cagtcacctg
    53581 gcggatctga ccagaagcca aatccattac gtacaggttc agactaccgg ttttcgacaa
    53641 ggcgaatgcc agtttgctgc cgtctggcga gaatgcaggt gcaccgttgt gacgcgggaa
    53701 tgaagccacc tgacgtacag cgccatttgc cagcgtctga ataaccagcg cggaacgacc
    53761 gctttcgaag gtcacataag ccagtttaga accgtctggt gaccacgccg gagacatcag
    53821 cggctgcggt gaacggtgaa cgacaaactg gttgtaaccg tcatagtcag atacgcgcag
    53881 ttcatacggg aactgaccgc cgttggtctg aacaacgtag gcaatacgag tacggaacgc
    53941 acctttaatg ccggtcagtt tttcaaacac ttcatcactg gcggtatgac cagcataacg
    54001 cagccactgc ttgttcactt tgtacgagtt ctgagcaagt acagtacccg gtgcgccgcc
    54061 agtgtcaaca agttgataag caacattgta agaaccatcc ggattcggag tgacctgacc
    54121 gacaactaca gcgtcaatgc ccagtgcgga ccatgcagct ggttgtactt cctgcgcact
    54181 acccggctgc tgtggcagac gagcgcgatc taacggatta aatttaccgc tgttacgcaa
    54241 gtcagcagca acgatgccgc caatatcttc aggtgccgca ccaggccccg cccactggaa
    54301 aggaacaaca ccaataggac gaccggaatc tacaccgctg tcgatcacaa tgcggacttc
    54361 agcatgcaga actgatgccc acagtatgag aaaaccaaat gctactcgta atgcctgctt
    54421 catcatatct cccttatctg gaccgatggc ccacgataat ttagcagaat gttaacaaac
    54481 taaaatacac aaaactacca gaaccccgtg gcaaccggtg cctgatgttg accgtccgaa
    54541 cagtcaacat cgcgattacg gtttgaagtc caatggcgcg tttttgaaca cttcatatac
    54601 tgcctggctt ggtggtttcg ggatcttcgc aagtttagct gctgccaacg cagcctgaca
    54661 aagtgcggga tcgccacctt caggtttgat atccagtaac ataccatcgg gtgccagttt
    54721 tatgcgcagc gtacaggttt tgcctgcata ggacgatgcg tcatagaact tactttcgat
    54781 agcagattta atctgcccgg catagttatt gatatcggcc cctgatgcgc cattgttttt
    54841 agtattacca ctcccggcag gcgaagcatt gttccctttc gcccctcccc ccgttttcgg
    54901 tgcattctta ccagagctta gctcaccgaa aatatcatct gcctctgcgg ccgcttttgc
    54961 tgcagcggct ttttcagctg cagcttttgc cgctgccgct tttttgtctg cggcggcttt
    55021 ttctgccgct gctttcttat cagctgctgc cttttcagca gccgcttttt ctgctgcttt
    55081 tttgtcggct gcagctttct ctgctgccgc tttcttatca gctgcagcct tttcagcagc
    55141 cgctttcttc tcagcttctg ctttggcttt ttcagcagct tcagttgccg ctttctttct
    55201 tgcttcagct gcagctgctt ctgccgcttc cgctttcttc ttcagtgccg cagcggctgc
    55261 ctcggctttt ttctgcgctt cggctgcggc tttggcggct tctgcttctg cttttttctt
    55321 tgcgtctgca gccgctttct tcgctgcttc ttccgcagct ttagcatctg cttcggcctt
    55381 cgctttagca tctgccgccg ctttcgctgc cgcctcttca gcttgcttct gctttaactc
    55441 ggcctgtttt gcggcttctt cagcctgctt tttctgctcc tgagccgcta accgctcttt
    55501 ctcaagttgc ttcaggcgtt cctgttcagc cgcttgtttc tcacgcagtt cttcagcagc
    55561 ctgctgttcc ttcatcttgc gctgttcatc agaacgcttc gcgcttgatt cctggctttg
    55621 catgcgtttg tactgctcaa ctaccgcacc tgaatcaacc atgacagcgt cgatggacga
    55681 accaccgccg cctccggctg aagcttctat attctcatcg aacgaactcc agatcagcgc
    55741 cgcaaataag atgacatgca gcactgctga aataattatc gcccgcttga gcttgtcgtt
    55801 ttgttcggtt gcctttgaca ctctcggttt ccaaaaactg ttcgcctgtt acccgctctc
    55861 tttcaagcaa gggaaacgca gatgtttaga taggctgcgt cattaaacca accgatttca
    55921 cacccgcact atgtaacaag ttcagtgctt taattatttc atcgtaaggc acatcttttg
    55981 cgccaccgat cagaaagacc gttttcgggt tggccttgaa acggctggac acttccgcca
    56041 ccacctgctc tggtggtaaa cgctccaggc gatctttctc aaccaccacg gtgtactgac
    56101 caataccaga cacttcaaca atcactggcg gattatcgtt actgctcacc gcctgtgatt
    56161 cagtagcgtc tggcagatcg acctccacgc tctgggtgat gatgggcgct gtcgccataa
    56221 agatcagcaa cagcaccagc agtacgtcca gcaacggtac aatgttgatt tcggacttga
    56281 gatcgcgacg acctcgtcca cgcgctctgg ccatggctta ccccttgttg ctctcgctaa
    56341 cggtaaacgc ctggcggtgc agaatcgcgg taaactcttc cataaagttg tcgtaattca
    56401 gttccagttt gtttacgcgc tggttgaggc ggttataagc cattaccgcc ggaattgcgg
    56461 cgaacagacc aattgcagtc gcaatcaacg cttctgcgat accgggcgca accatttgca
    56521 gtgttgcttg ttttaccgcc ccgagggcga taaaggcgtg catgatcccc cagaccgtac
    56581 caaacagacc aatatacggg ctgatggagc caaccgtacc gaggaacgga atgtgcgttt
    56641 ccagattttc aagttcacgg ttcatggaga tacgcatagc acgcgacgcc ccttccacta
    56701 cggcttccgg cgcatggcta ttggcacgat gcaggcgcac aaactctttg aacccgctgt
    56761 aaaagatttg ttccgaacca gtcagattat cccgtttccc ctggctctct tgatagaggc
    56821 gagagagttc gattccagac cagaatttat cttcaaacgc ttcggcttcg cgcgccgctg
    56881 cgttaagaat acgggtccgc tggataataa tggcccaaga tgcgattgaa aaaccaatca
    56941 aaatcaacat gataagttta accagaaggc tagccttcag gaacaaatca aggatattca
    57001 tgtcagtcac tgcttaaact ccgcgacaat agacttggga agcgcacgag gcttcatttt
    57061 gagtgggtca acgcaaacaa ccagaacctc tgcttcattc agcaaagtat tctcggcgtt
    57121 gacaatacgt tgcgtgaaaa ccaaagaggt gccacgcatt gatgttattt cagtctgtat
    57181 ttcgagcata tcgtcgagcc gcgcaggtgc gtaatattcc accgtcattt tacgtaccac
    57241 aaaggcaacg cgttcagcca tcagcgcctg ctgactgaag tgatgatgac gcagcatctc
    57301 tgtgcgtgct ctttcataaa aagcgacgta actggcgtgg tacaccacac caccggcatc
    57361 ggtatcttca tagtagacgc gaaccggcca tcgaaacagc gttgtattca ctttacatcc
    57421 cggtaatgca acaaaagtta gagcttttaa acgctgctac tatacgcgcg cgattgaggt
    57481 ttgggaatga gggcaagtta agggagcgta aaaatttatg ggcctggagc ggcccataat
    57541 tttttaaaga gaattctgat ttaaaagaag aagaaaatca gcccgacaat gagaacaata
    57601 tcggcaagca atgggcaaaa aatcccttgc caaagaacct tctgcggacg aaagcccacg
    57661 ccgtgaatca caccagcaca gacggcccac atcagcaata aaccatgcca gatttccagt
    57721 tcactggtct tcgcggcaaa acgagatggg tcccaaaaca tacatcctgc taacagaagc
    57781 gccatcacga aggaaagcgc ccgcaggggg cgcttgtcca ttaccgcata caaagtcgcg
    57841 ataatcttac tcatcagatg tcttcttgac cggctttgcc tgattcgacg tgttcaagcg
    57901 ccagcgcggt gattacccca aacgaacagg caagaagcgt tcccagaatc catgcgaaat
    57961 accacatttt tagctcctta cttagtacag agagtgggtg ttacgttcaa tatcttcttt
    58021 ggtgatacga ccgaacattt tccagtaaca ccaggcggtg tagagcagaa tgatcggtac
    58081 cagaaccacc gcaacccagg tcatgacgtt aagcgtcagc tggctggaag ttgcatccca
    58141 cattgtcaga cttgcgttca tcatggtgct ggacggcatc acaaacggga acattgcgat
    58201 accggctgtc aggatgatgc aggccagcgt cagggaggag aacacaaacg cccacgcggc
    58261 tttatccata cgtgcagtca ggatggtcag cagcggcaga accacaccca gcgccggaat
    58321 agcccacaga attggcgtgt tgttgaagtt aaccagccat gcgccagctt cacgaaccac
    58381 ttctttattc agtgggttag aggctgcgta atggtccatt gtcgatttca cgacataacc
    58441 atcgataccg tacatcaccc atacgccagc cagtgcgaaa cagaccagtg tcaccagcgc
    58501 agccacctga gccgttgcac gggtacgcag gtgcagttcg cccacggtac gcatttgcag
    58561 ataggttgcg ccctgagtaa tgatcatccc tacgctcacc acgcctgcca gcaggccgaa
    58621 cgggttaagc aactggaaga agttaccggt gtagtacaga cgcagatatt catcaacgtt
    58681 gaacggtacg ccctgcaaca ggttaccgaa cgctacacca attaccagcg gcggaacgaa
    58741 gctaccaatg aagatgcccc agtcccacat gttacgccag cgggtttctt caatcttgga
    58801 gcggtagtca aaaccgaccg gacggaagaa caaagacgcc agcacgagga tcatcgccac
    58861 atagaagccg gagaacgcag cggcatagac catcggccag gcagcaaaga gtgcgccgcc
    58921 cgcggtgatc agccaaacct ggttaccgtc ccagtgtggt gcaatggagt taatcataat
    58981 tcgacgctcg gtgtcgttac gaccgaggaa acgggtgagc atgcccaccc ccatgtcgaa
    59041 accgtcagtg actgcaaaac caatcagcag aacgccaacc agcagccacc agataaaacg
    59101 caatacttca taatcgatca tttgacgact cctgtcttag cgtgccggct gagtagtcgt
    59161 ggaagactgc tcaaagtgat agcgaccggt tttcaggctg cttgggccga ggcgtgcaaa
    59221 tttgaacatt aagaacaatt ctgccaccag gaacagggta tacaggccgc aaatcagcac
    59281 cattgagaag atgagatcgc ctgcggtcag tgacgagttc gccacagctg tcggcagcac
    59341 ttcaccgata gcccacggtt ggcggccata ttcagccacg aaccagcccg cttctacagc
    59401 aatccacggc agcggaatac cgtacagcgc ggcgcgcaga agccattttt tctcgccaat
    59461 gcggttgcgg atgacactcc agaaagagag cgcgatgatt gccagcagca ggaagccaca
    59521 cgccaccatg atacggaacg caaagtacag cggcgctaca cgcgggatgg agtctttggt
    59581 tgcctgttga atctgcgctt cagtcgcatc agccacgttt ggcgtatagc gtttcagcag
    59641 cagaccgtaa ccgaggtctt tcttcatgct attgaactgg tcacgaaccg cctggtcggt
    59701 agaaccagaa cgcagttgtt cgagcagaga gtacgccttc atcccgttac gaatgcgttc
    59761 ttcatgctgc accatcagct ctttcaggcc gataaccggg gtatccacgg aacgcgttgc
    59821 aatgatgccc agtgcgtaag ggatctgaat cgcaaatttg ttcgtctcct cttcctgatc
    59881 aggaatgccg aacagagtaa aggcagcagg cgcaggttgc gtttcccact cggcttcaat
    59941 agcagccagt ttggttttct gcacgtcgcc catttcgtag ccggattcat cacccagaac
    60001 aataacagac agaacagcag ccataccgaa gctggcagcg atagcaaagg agcgtttagc
    60061 gaaggcgaag tcacgacctt tcagcatata ccatgcgctg ataccgagga tgaacatcgc
    60121 gccagtcaca taaccagacg ctacagtgtg aacgaatttc acctgagcaa ccgggttaag
    60181 caccagctcg gagaagctca ccatctccat acgcatagtt tcaaagttga aatcggacgc
    60241 gattgggttt tgcatccagc cgttcgcaac cagaatccac agtgcggaca ggtttgaacc
    60301 gagcgccacc agccaggtga cacacatatg ctgaacttta cccagacgat cccaaccgaa
    60361 gaagaacaga cctacaaagg tggattcgag gaagaaggcc atcagacctt cgattgccag
    60421 cggcgcaccg aagatatccc ctacatagtg ggaatagtaa gaccagttag tcccgaactg
    60481 gaactccatg gtcagaccgg tagccacacc cagagcgaag ttgataccaa acaacttgcc
    60541 ccagaacttg gtcatatctt tataaatctg tttgccggag aggacgtaga ccgtttccat
    60601 aatggccagc aggaacgcca taccgagcgt cagtggcaca aaaaggaagt ggtacatcgc
    60661 ggtcaaggca aactgtaagc gcgacagttc gactatatct aacatcatga ctccttgctc
    60721 atcgcatgaa gactccgaga gtgaaccccg ttagaaaggg tcacacgcat gccccaatag
    60781 aaatttgttt gaacactttc atcataccat gtgaatgcac acaaaaacgg cgtaaagaaa
    60841 ggttgcaaac atgttaataa aaactcaaat tgatcccacg tatatattac gccgcaaaat
    60901 ccttacaata aacaggtttt tgttgaagca aatttgcatt tttcgacggt gatcaattta
    60961 tagctaaatt accgcctttc agccaatttg atcgagaaca atttatctct ttttgatgcc
    61021 catttccaag acttatacat tgataaatat caattcctac aaattatgtt aatcatgttt
    61081 caataaggta actttagtga catttatgtt taaaatgtgt gagttataag gctgaaatta
    61141 gtttcggttg gcaattaaag ataaaaagag acgaagcaaa aacagcacgg ttgaccttaa
    61201 ttcttttttt ggtcactggc taatacgacg cagagtttca ggattactaa taaaggcata
    61261 aatcca
//
LOCUS       DAZGAN020000015        59451 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGAN020000015 DAZGAN020000000
VERSION     DAZGAN020000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 59451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.015"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            60..1469
                     /gene="ygcS"
                     /locus_tag="ACHKYO_RS05515"
                     /old_locus_tag="pgaptmp_001103"
     CDS             60..1469
                     /gene="ygcS"
                     /locus_tag="ACHKYO_RS05515"
                     /old_locus_tag="pgaptmp_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4427984.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            1447..2226
                     /gene="ygcR"
                     /locus_tag="ACHKYO_RS05520"
                     /old_locus_tag="pgaptmp_001104"
     CDS             1447..2226
                     /gene="ygcR"
                     /locus_tag="ACHKYO_RS05520"
                     /old_locus_tag="pgaptmp_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGU4427985.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWRDYLRQRMQP"
     gene            2223..3083
                     /gene="ygcQ"
                     /locus_tag="ACHKYO_RS05525"
                     /old_locus_tag="pgaptmp_001105"
     CDS             2223..3083
                     /gene="ygcQ"
                     /locus_tag="ACHKYO_RS05525"
                     /old_locus_tag="pgaptmp_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU4427986.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(3231..3806)
                     /gene="ygcP"
                     /locus_tag="ACHKYO_RS05530"
                     /old_locus_tag="pgaptmp_001106"
     CDS             complement(3231..3806)
                     /gene="ygcP"
                     /locus_tag="ACHKYO_RS05530"
                     /old_locus_tag="pgaptmp_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HGU4427987.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(3823..4083)
                     /gene="ygcO"
                     /locus_tag="ACHKYO_RS05535"
                     /old_locus_tag="pgaptmp_001107"
     CDS             complement(3823..4083)
                     /gene="ygcO"
                     /locus_tag="ACHKYO_RS05535"
                     /old_locus_tag="pgaptmp_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU4427988.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLISACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4074..5345)
                     /gene="ygcN"
                     /locus_tag="ACHKYO_RS05540"
                     /old_locus_tag="pgaptmp_001108"
     CDS             complement(4074..5345)
                     /gene="ygcN"
                     /locus_tag="ACHKYO_RS05540"
                     /old_locus_tag="pgaptmp_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4427989.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERCITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(5423..5785)
                     /gene="queD"
                     /locus_tag="ACHKYO_RS05545"
                     /old_locus_tag="pgaptmp_001109"
     CDS             complement(5423..5785)
                     /gene="queD"
                     /locus_tag="ACHKYO_RS05545"
                     /old_locus_tag="pgaptmp_001109"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HGU4427990.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6104..7903
                     /gene="cysJ"
                     /locus_tag="ACHKYO_RS05550"
                     /old_locus_tag="pgaptmp_001110"
     CDS             6104..7903
                     /gene="cysJ"
                     /locus_tag="ACHKYO_RS05550"
                     /old_locus_tag="pgaptmp_001110"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HGU4427991.1"
                     /translation="MTTQVPPSALLPLNPEQLVRLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            7903..9615
                     /gene="cysI"
                     /locus_tag="ACHKYO_RS05555"
                     /old_locus_tag="pgaptmp_001111"
     CDS             7903..9615
                     /gene="cysI"
                     /locus_tag="ACHKYO_RS05555"
                     /old_locus_tag="pgaptmp_001111"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HGU4427992.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDHEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            9689..10423
                     /gene="cysH"
                     /locus_tag="ACHKYO_RS05560"
                     /old_locus_tag="pgaptmp_001112"
     CDS             9689..10423
                     /gene="cysH"
                     /locus_tag="ACHKYO_RS05560"
                     /old_locus_tag="pgaptmp_001112"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU4427993.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            10688..10840
                     /locus_tag="ACHKYO_RS05565"
                     /old_locus_tag="pgaptmp_001113"
     CDS             10688..10840
                     /locus_tag="ACHKYO_RS05565"
                     /old_locus_tag="pgaptmp_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4427994.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11035..13734
                     /locus_tag="ACHKYO_RS05570"
                     /old_locus_tag="pgaptmp_001114"
     CDS             11035..13734
                     /locus_tag="ACHKYO_RS05570"
                     /old_locus_tag="pgaptmp_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HGU4427995.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKY
                     CSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            13832..15394
                     /gene="casA"
                     /locus_tag="ACHKYO_RS05575"
                     /old_locus_tag="pgaptmp_001115"
                     /gene_synonym="cse1"
     CDS             13832..15394
                     /gene="casA"
                     /locus_tag="ACHKYO_RS05575"
                     /old_locus_tag="pgaptmp_001115"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HGU4427996.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKNHGRWDDIWQDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATSIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPYELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQEAAE"
     gene            15391..15924
                     /gene="casB"
                     /locus_tag="ACHKYO_RS05580"
                     /old_locus_tag="pgaptmp_001116"
                     /gene_synonym="cse2"
     CDS             15391..15924
                     /gene="casB"
                     /locus_tag="ACHKYO_RS05580"
                     /old_locus_tag="pgaptmp_001116"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HGU4427997.1"
                     /translation="MSIVKDEHKVALRKWHEELQDKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMKKGRFTRLL
                     AVKTPDDLLRQLRRAVKLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQN"
     gene            15939..17012
                     /gene="cas7e"
                     /locus_tag="ACHKYO_RS05585"
                     /old_locus_tag="pgaptmp_001117"
     CDS             15939..17012
                     /gene="cas7e"
                     /locus_tag="ACHKYO_RS05585"
                     /old_locus_tag="pgaptmp_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HGU4427998.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDSGSPKTVILGGATRLRVSSQSL
                     KRAWRTSELFEQALAGHIGVRSGRIAREAATILIEKGIEDKKAIEWAVEIADYLGKAK
                     KDKKQKNDKKPKDPLTSAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEKDLALLRK
                     DRMAVDIAMFGRMLAKKPEFNVEAACQVAHAFGVSETIVENDFFTAVDDLRQASEDAG
                     AGHVDETGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNS
                     FASRAYASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKT
                     ECASFDVMNKQGSMKDVLDFICA"
     gene            17023..17769
                     /gene="cas5e"
                     /locus_tag="ACHKYO_RS05590"
                     /old_locus_tag="pgaptmp_001118"
     CDS             17023..17769
                     /gene="cas5e"
                     /locus_tag="ACHKYO_RS05590"
                     /old_locus_tag="pgaptmp_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HGU4427999.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            17751..18401
                     /gene="cas6e"
                     /locus_tag="ACHKYO_RS05595"
                     /old_locus_tag="pgaptmp_001119"
     CDS             17751..18401
                     /gene="cas6e"
                     /locus_tag="ACHKYO_RS05595"
                     /old_locus_tag="pgaptmp_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HGU4428000.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            18398..19318
                     /gene="cas1e"
                     /locus_tag="ACHKYO_RS05600"
                     /old_locus_tag="pgaptmp_001120"
     CDS             18398..19318
                     /gene="cas1e"
                     /locus_tag="ACHKYO_RS05600"
                     /old_locus_tag="pgaptmp_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HGU4428001.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            19318..19611
                     /gene="cas2e"
                     /locus_tag="ACHKYO_RS05605"
                     /old_locus_tag="pgaptmp_001121"
     CDS             19318..19611
                     /gene="cas2e"
                     /locus_tag="ACHKYO_RS05605"
                     /old_locus_tag="pgaptmp_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HGU4428002.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     repeat_region   19708..20102
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19708..19736
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaaccg"
     gene            complement(20184..21221)
                     /gene="iap"
                     /locus_tag="ACHKYO_RS05610"
                     /old_locus_tag="pgaptmp_001122"
     CDS             complement(20184..21221)
                     /gene="iap"
                     /locus_tag="ACHKYO_RS05610"
                     /old_locus_tag="pgaptmp_001122"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HGU4428003.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            21473..22381
                     /gene="cysD"
                     /locus_tag="ACHKYO_RS05615"
                     /old_locus_tag="pgaptmp_001123"
     CDS             21473..22381
                     /gene="cysD"
                     /locus_tag="ACHKYO_RS05615"
                     /old_locus_tag="pgaptmp_001123"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HGU4428004.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            22383..23810
                     /gene="cysN"
                     /locus_tag="ACHKYO_RS05620"
                     /old_locus_tag="pgaptmp_001124"
     CDS             22383..23810
                     /gene="cysN"
                     /locus_tag="ACHKYO_RS05620"
                     /old_locus_tag="pgaptmp_001124"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HGU4428005.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            23810..24415
                     /gene="cysC"
                     /locus_tag="ACHKYO_RS05625"
                     /old_locus_tag="pgaptmp_001125"
     CDS             23810..24415
                     /gene="cysC"
                     /locus_tag="ACHKYO_RS05625"
                     /old_locus_tag="pgaptmp_001125"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HGU4428006.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            24465..24788
                     /gene="ygbE"
                     /locus_tag="ACHKYO_RS05630"
                     /old_locus_tag="pgaptmp_001126"
     CDS             24465..24788
                     /gene="ygbE"
                     /locus_tag="ACHKYO_RS05630"
                     /old_locus_tag="pgaptmp_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HGU4428007.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            24982..25293
                     /gene="ftsB"
                     /locus_tag="ACHKYO_RS05635"
                     /old_locus_tag="pgaptmp_001127"
     CDS             24982..25293
                     /gene="ftsB"
                     /locus_tag="ACHKYO_RS05635"
                     /old_locus_tag="pgaptmp_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HGU4428008.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            25312..26022
                     /gene="ispD"
                     /locus_tag="ACHKYO_RS05640"
                     /old_locus_tag="pgaptmp_001128"
     CDS             25312..26022
                     /gene="ispD"
                     /locus_tag="ACHKYO_RS05640"
                     /old_locus_tag="pgaptmp_001128"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGU4428009.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            26022..26501
                     /gene="ispF"
                     /locus_tag="ACHKYO_RS05645"
                     /old_locus_tag="pgaptmp_001129"
     CDS             26022..26501
                     /gene="ispF"
                     /locus_tag="ACHKYO_RS05645"
                     /old_locus_tag="pgaptmp_001129"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HGU4428010.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            26498..27547
                     /gene="truD"
                     /locus_tag="ACHKYO_RS05650"
                     /old_locus_tag="pgaptmp_001130"
     CDS             26498..27547
                     /gene="truD"
                     /locus_tag="ACHKYO_RS05650"
                     /old_locus_tag="pgaptmp_001130"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HGU4428011.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            27528..28289
                     /gene="surE"
                     /locus_tag="ACHKYO_RS05655"
                     /old_locus_tag="pgaptmp_001131"
     CDS             27528..28289
                     /gene="surE"
                     /locus_tag="ACHKYO_RS05655"
                     /old_locus_tag="pgaptmp_001131"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HGU4428012.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            28283..28909
                     /gene="pcm"
                     /locus_tag="ACHKYO_RS05660"
                     /old_locus_tag="pgaptmp_001132"
     CDS             28283..28909
                     /gene="pcm"
                     /locus_tag="ACHKYO_RS05660"
                     /old_locus_tag="pgaptmp_001132"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HGU4428013.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            29094..30188
                     /gene="nlpD"
                     /locus_tag="ACHKYO_RS05665"
                     /old_locus_tag="pgaptmp_001133"
     CDS             29094..30188
                     /gene="nlpD"
                     /locus_tag="ACHKYO_RS05665"
                     /old_locus_tag="pgaptmp_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HGU4428014.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            30251..31243
                     /gene="rpoS"
                     /locus_tag="ACHKYO_RS05670"
                     /old_locus_tag="pgaptmp_001134"
     CDS             30251..31243
                     /gene="rpoS"
                     /locus_tag="ACHKYO_RS05670"
                     /old_locus_tag="pgaptmp_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HGU4428015.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(31364..31771)
                     /locus_tag="ACHKYO_RS05675"
                     /old_locus_tag="pgaptmp_001135"
     CDS             complement(31364..31771)
                     /locus_tag="ACHKYO_RS05675"
                     /old_locus_tag="pgaptmp_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGU4428016.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            31918..32511
                     /locus_tag="ACHKYO_RS05680"
                     /old_locus_tag="pgaptmp_001136"
     CDS             31918..32511
                     /locus_tag="ACHKYO_RS05680"
                     /old_locus_tag="pgaptmp_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HGU4428017.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            32511..33938
                     /locus_tag="ACHKYO_RS05685"
                     /old_locus_tag="pgaptmp_001137"
     CDS             32511..33938
                     /locus_tag="ACHKYO_RS05685"
                     /old_locus_tag="pgaptmp_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HGU4428018.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFCLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            33949..34185
                     /locus_tag="ACHKYO_RS05690"
                     /old_locus_tag="pgaptmp_001138"
     CDS             33949..34185
                     /locus_tag="ACHKYO_RS05690"
                     /old_locus_tag="pgaptmp_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HGU4428019.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(34224..35588)
                     /gene="ygbN"
                     /locus_tag="ACHKYO_RS05695"
                     /old_locus_tag="pgaptmp_001139"
     CDS             complement(34224..35588)
                     /gene="ygbN"
                     /locus_tag="ACHKYO_RS05695"
                     /old_locus_tag="pgaptmp_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HGU4428020.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(35677..36453)
                     /gene="ygbM"
                     /locus_tag="ACHKYO_RS05700"
                     /old_locus_tag="pgaptmp_001140"
     CDS             complement(35677..36453)
                     /gene="ygbM"
                     /locus_tag="ACHKYO_RS05700"
                     /old_locus_tag="pgaptmp_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HGU4428021.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(36458..37096)
                     /gene="ygbL"
                     /locus_tag="ACHKYO_RS05705"
                     /old_locus_tag="pgaptmp_001141"
     CDS             complement(36458..37096)
                     /gene="ygbL"
                     /locus_tag="ACHKYO_RS05705"
                     /old_locus_tag="pgaptmp_001141"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HGU4428022.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(37093..38355)
                     /gene="otnK"
                     /locus_tag="ACHKYO_RS05710"
                     /old_locus_tag="pgaptmp_001142"
     CDS             complement(37093..38355)
                     /gene="otnK"
                     /locus_tag="ACHKYO_RS05710"
                     /old_locus_tag="pgaptmp_001142"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HGU4428023.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(38352..39260)
                     /gene="ltnD"
                     /locus_tag="ACHKYO_RS05715"
                     /old_locus_tag="pgaptmp_001143"
     CDS             complement(38352..39260)
                     /gene="ltnD"
                     /locus_tag="ACHKYO_RS05715"
                     /old_locus_tag="pgaptmp_001143"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HGU4428024.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            39456..40223
                     /gene="ygbI"
                     /locus_tag="ACHKYO_RS05720"
                     /old_locus_tag="pgaptmp_001144"
     CDS             39456..40223
                     /gene="ygbI"
                     /locus_tag="ACHKYO_RS05720"
                     /old_locus_tag="pgaptmp_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HGU4428025.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASLALAKQDLSLLVAKNE"
     gene            complement(40274..40930)
                     /gene="pphB"
                     /locus_tag="ACHKYO_RS05725"
                     /old_locus_tag="pgaptmp_001145"
     CDS             complement(40274..40930)
                     /gene="pphB"
                     /locus_tag="ACHKYO_RS05725"
                     /old_locus_tag="pgaptmp_001145"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU4428026.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFYPETD
                     LLISTGDNIDRGPKSLNVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(41036..43597)
                     /gene="mutS"
                     /locus_tag="ACHKYO_RS05730"
                     /old_locus_tag="pgaptmp_001146"
     CDS             complement(41036..43597)
                     /gene="mutS"
                     /locus_tag="ACHKYO_RS05730"
                     /old_locus_tag="pgaptmp_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGU4428027.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHSDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43673..43852
                     /locus_tag="ACHKYO_RS05735"
                     /old_locus_tag="pgaptmp_001147"
     CDS             43673..43852
                     /locus_tag="ACHKYO_RS05735"
                     /old_locus_tag="pgaptmp_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4428028.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            43884..44237
                     /gene="ygbA"
                     /locus_tag="ACHKYO_RS05740"
                     /old_locus_tag="pgaptmp_001148"
     CDS             43884..44237
                     /gene="ygbA"
                     /locus_tag="ACHKYO_RS05740"
                     /old_locus_tag="pgaptmp_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HGU4428029.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(44274..46352)
                     /gene="flhA"
                     /locus_tag="ACHKYO_RS05745"
                     /old_locus_tag="pgaptmp_001149"
     CDS             complement(44274..46352)
                     /gene="flhA"
                     /locus_tag="ACHKYO_RS05745"
                     /old_locus_tag="pgaptmp_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HGU4428030.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(46426..47436)
                     /gene="hypE"
                     /locus_tag="ACHKYO_RS05750"
                     /old_locus_tag="pgaptmp_001150"
     CDS             complement(46426..47436)
                     /gene="hypE"
                     /locus_tag="ACHKYO_RS05750"
                     /old_locus_tag="pgaptmp_001150"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HGU4428031.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLSMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDMLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(47433..48554)
                     /gene="hypD"
                     /locus_tag="ACHKYO_RS05755"
                     /old_locus_tag="pgaptmp_001151"
     CDS             complement(47433..48554)
                     /gene="hypD"
                     /locus_tag="ACHKYO_RS05755"
                     /old_locus_tag="pgaptmp_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HGU4428032.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(48554..48826)
                     /gene="hypC"
                     /locus_tag="ACHKYO_RS05760"
                     /old_locus_tag="pgaptmp_001152"
     CDS             complement(48554..48826)
                     /gene="hypC"
                     /locus_tag="ACHKYO_RS05760"
                     /old_locus_tag="pgaptmp_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HGU4428033.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(48817..49689)
                     /gene="hypB"
                     /locus_tag="ACHKYO_RS05765"
                     /old_locus_tag="pgaptmp_001153"
     CDS             complement(48817..49689)
                     /gene="hypB"
                     /locus_tag="ACHKYO_RS05765"
                     /old_locus_tag="pgaptmp_001153"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HGU4428034.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(49693..50043)
                     /gene="hypA"
                     /locus_tag="ACHKYO_RS05770"
                     /old_locus_tag="pgaptmp_001154"
     CDS             complement(49693..50043)
                     /gene="hypA"
                     /locus_tag="ACHKYO_RS05770"
                     /old_locus_tag="pgaptmp_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU4428035.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            50255..50716
                     /gene="hycA"
                     /locus_tag="ACHKYO_RS05775"
                     /old_locus_tag="pgaptmp_001155"
     CDS             50255..50716
                     /gene="hycA"
                     /locus_tag="ACHKYO_RS05775"
                     /old_locus_tag="pgaptmp_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HGU4428036.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            50841..51452
                     /gene="hycB"
                     /locus_tag="ACHKYO_RS05780"
                     /old_locus_tag="pgaptmp_001156"
     CDS             50841..51452
                     /gene="hycB"
                     /locus_tag="ACHKYO_RS05780"
                     /old_locus_tag="pgaptmp_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HGU4428037.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            51449..53275
                     /gene="hycC"
                     /locus_tag="ACHKYO_RS05785"
                     /old_locus_tag="pgaptmp_001157"
     CDS             51449..53275
                     /gene="hycC"
                     /locus_tag="ACHKYO_RS05785"
                     /old_locus_tag="pgaptmp_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HGU4428038.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGSAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            53278..54201
                     /gene="hycD"
                     /locus_tag="ACHKYO_RS05790"
                     /old_locus_tag="pgaptmp_001158"
     CDS             53278..54201
                     /gene="hycD"
                     /locus_tag="ACHKYO_RS05790"
                     /old_locus_tag="pgaptmp_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HGU4428039.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            54219..55928
                     /gene="hycE"
                     /locus_tag="ACHKYO_RS05795"
                     /old_locus_tag="pgaptmp_001159"
     CDS             54219..55928
                     /gene="hycE"
                     /locus_tag="ACHKYO_RS05795"
                     /old_locus_tag="pgaptmp_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HGU4428040.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            55938..56480
                     /gene="hycF"
                     /locus_tag="ACHKYO_RS05800"
                     /old_locus_tag="pgaptmp_001160"
     CDS             55938..56480
                     /gene="hycF"
                     /locus_tag="ACHKYO_RS05800"
                     /old_locus_tag="pgaptmp_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HGU4428041.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            56480..57247
                     /gene="hycG"
                     /locus_tag="ACHKYO_RS05805"
                     /old_locus_tag="pgaptmp_001161"
     CDS             56480..57247
                     /gene="hycG"
                     /locus_tag="ACHKYO_RS05805"
                     /old_locus_tag="pgaptmp_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HGU4428042.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            57244..57654
                     /gene="hycH"
                     /locus_tag="ACHKYO_RS05810"
                     /old_locus_tag="pgaptmp_001162"
     CDS             57244..57654
                     /gene="hycH"
                     /locus_tag="ACHKYO_RS05810"
                     /old_locus_tag="pgaptmp_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HGU4428043.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            57647..58117
                     /gene="hycI"
                     /locus_tag="ACHKYO_RS05815"
                     /old_locus_tag="pgaptmp_001163"
     CDS             57647..58117
                     /gene="hycI"
                     /locus_tag="ACHKYO_RS05815"
                     /old_locus_tag="pgaptmp_001163"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HGU4428044.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE"
     gene            58160..58915
                     /locus_tag="ACHKYO_RS05820"
                     /old_locus_tag="pgaptmp_001164"
     CDS             58160..58915
                     /locus_tag="ACHKYO_RS05820"
                     /old_locus_tag="pgaptmp_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4428045.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQNWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGVLQNMAGNAQLYRAD
                     LDRNGIQDLVIWRGISGNGLAPNAFLILMTFNQQGRPCVFQSDGFYTASETGIDDLLD
                     LQRNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLYGVNQN"
     gene            58986..59405
                     /locus_tag="ACHKYO_RS05825"
                     /old_locus_tag="pgaptmp_001165"
     CDS             58986..59405
                     /locus_tag="ACHKYO_RS05825"
                     /old_locus_tag="pgaptmp_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4428046.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
BASE COUNT        13947 a        15187 c        16250 g        14067 t
ORIGIN      
        1 ttatgaatac gggtactatc tatccgattg aaaaataatg tgtcaggcag cgttccgcga
       61 tgacggggcg ttgccttttc ggcttctcag gcgagaagcc gttcttatta ccggacaatg
      121 aaggggtaaa gatgaacact tcaccggtgc gaatggatga tttaccgctt aaccgttttc
      181 actgccgcat tgctgcgctc actttcggcg cacacctgac cgacggttat gttctcggcg
      241 tcattggtta cgccattatt cagcttacgc ccgccatgca actgacgccg tttatggcgg
      301 gaatgatcgg cggctcggcg ctccttggtt tgttccttgg cagcctggtt cttggatgga
      361 tctccgacca tattggtcgg caaaaaatct tcaccttcag ctttttgctg attacgcttg
      421 cttcgttttt acaatttttt gccaccacgc cagagcatct tattggactg cgcattttga
      481 ttggcattgg tctgggaggc gattattcag taggtcacac cttgctggct gaattttccc
      541 cgcgccgcca tcgcggtatt ttgctgggcg cattcagcgt ggtgtggacc gtaggctatg
      601 tgctggcaag tattgccgga catcacttta tttccgaaaa cccggaggcc tggcgctggc
      661 tactggcatc ggcagctctg cccgcgttgt tgattacgtt attacgctgg ggaacgccag
      721 aatcaccacg ctggctactg cgccaggggc gttttgcaga agctcacgct atcgtgcatc
      781 gctattttgg tccccatgtt ttactgggcg atgaagtggt aacggcgacc cataaacaca
      841 tcaaaacctt gttctcttcg cgttactggc ggcgcacggc gtttaacagc gtcttctttg
      901 tctgcctcgt aatcccatgg tttgtgattt atacctggct gccaactatc gcccagacta
      961 ttggtctgga agatgcgctg actgccagcc tgatgcttaa tgcgttgtta attgtgggcg
     1021 cgctgctggg attagttctg acgcacctgc tggcacatcg caaatttttg ctgggaagtt
     1081 ttttgctgct ggcggcaacg ctggtagtaa tggcctgttt gccttccggc agttcattaa
     1141 cgctgctgct ttttgttctc ttcagcacca ccatttcggc agtcagtaat ctggtgggca
     1201 ttttgcctgc ggaaagtttt cctactgaca ttcgctcgct gggcgtcggt tttgccaccg
     1261 ccatgagtcg acttggcgcg gcggtaagta ctggcctgct gccgtgggtg ctggcgcagt
     1321 ggggaatgca agtcacctta ttgctcctgg cgacagtgtt gttggttggt tttgttgtga
     1381 cctggctatg ggcaccagaa actaaagccc tcccgctggt ggcggcggga aatgtaggag
     1441 gtgcgaatga acattctgtt agcgtttaaa gccgaaccgg atgccggaat gctggcggaa
     1501 aaagagtggc aggcggcggc tcagggtaaa agcggaccgg atatttcgct actgcgaagt
     1561 ttactcggtg ctgatgaaca ggccgctgcc gcgctgctgt tggcgcagag aaaaaacggt
     1621 acgccgatgt ctttaaccgc gttgagcatg ggggatgaac gggcattgca ctggctgcgc
     1681 tatctcatgg ctcttgggtt tgaggaagct gtattgctgg agacggcggc agatctgcgc
     1741 tttgcgccgg aatttgtcgc tcgccatatt gccgaatggc agcatcagaa tccgctggat
     1801 ctgataatta ccggctgcca aagcagcgaa gggcagaatg ggcaaacgcc atttttgctg
     1861 gcggaaatgc tgggctggcc ctgcttcacc caggtggagc gtttcactct cgacgcgctg
     1921 tttatcaccc tcgaacagcg tactgaacat gggttgcgtt gttgccgggt tcgcttgcct
     1981 gcggtgattg ccgtgcgtca gtgcggtgaa gtggccttgc cagtaccggg aatgcgccag
     2041 cgaatggctg cggggaaagc agaaattatc cgtaaaactg ttgctgccga aatgccagcg
     2101 atgcagtgtc tgcaactggc aagagcagag caacgacgcg gagcgacgct gattgatggg
     2161 caaacggtag cagaaaaagc tcaaaagcta tggcgggact atttacgcca aaggatgcag
     2221 ccatgaatat cgcaattgtg acaataaatc aggagaatgc cgcaatagca agctggctgg
     2281 cggcgcagga tttcagcggt tgtaccctgg cgcactggca gattgaacct cagcctgtag
     2341 tggcggaaca ggtgctggat gcgttggtgg aacagtggca gcgaacgcct gcggacgttg
     2401 ttctctttcc gccgggaacg tttggcgatg aactgtcgac ccggctggcc tggcgtttac
     2461 atggtgcaag catctgccag gtgacttcgt tagatattcc aaccgtaagc gtgcgcaaat
     2521 cccactgggg aaatgcgcta acggcgacgc tgcaaacaga aaaacgcccg ctatgccttt
     2581 ccctggcgcg tcaggcggga gcagcgaaaa acgccacgtt gccctctgga atgcaacaac
     2641 tgaacattgt cccgggtgcg cttccggact ggctggttag cacagaggat ctgaaaaatg
     2701 ttactcgcga cccattagct gaggccagac gggtgctggt ggtggggcag ggcggagagg
     2761 cagataacca ggaaatcgct atgctggcag aaaagctggg agccgaagtg ggctacagtc
     2821 gggcgcgagt gatgaacggt ggcgttgatg ctgaaaaagt gattggtatt tccggacatc
     2881 tgctggcacc tgaggtgtgt attgttgttg gcgcttctgg cgcagcagcg ttaatggcgg
     2941 gcgtacgcaa tagcaagttt gtggtggcga ttaatcatga cgccagcgct gcggtatttt
     3001 cacaggcaga tgttggcgtg gttgatgact ggaaagtggt gctggaggca ctggtgacaa
     3061 acattcacgc agattgccaa taacgtgttg ccggatgatc ctgcctacgg ttctggcctg
     3121 gggacttgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcgctgaa tgtcggatgt
     3181 ggcgtgcggc ctacaaatca aaccaaattc aattggttac gattatcccg tcaaagtaat
     3241 tttttcgcta acgtccagac cccggtattc gtggtggaaa gcgccacgac acccgcgtta
     3301 atcgcattac gcgcatcttc ttcatcgcac accagcccac cggcaatcag cggttggcgg
     3361 attttctctg tcacccagcc cagcactttg ggcatacagc ctggcaggat ctcaatacaa
     3421 tccggattcg attgcgcaac ttgcttatca atgttgtgaa acgaaattga atcaacaata
     3481 aacaggcgat gaatacagaa aaaaccttcc gctcttgccg ctttcaacat tgaggctttg
     3541 gtactgataa tgccgtccgc ctcggtcacc agtttcaaaa actgaataac gacttcttta
     3601 tttgacgcgc cttccagcaa atcaacatga ataaaagcat atttcccggc gttcttaatc
     3661 tttttaacga tattactgat ggtgcagata ttgccgtaca acacggaaat aaattggcat
     3721 tcagaatcga ttgccagttg caggctggca ttatctttaa cggcagcaat caccggattc
     3781 tggcggagca ggtgtaagag gggcataaat tatccttatt atttatccaa agcggtaggt
     3841 gatgccgaat ccggaaggcg gatagcgcca ctgttgtagt gttgattcgt cgcacagcaa
     3901 acggcaggtg ccacactcca ggcaactgcg atagtcaata cgtaagtcac cttccggtgt
     3961 gagcgaaaaa agacctgccg gacaggcgct aatcaaccgt tctgccgtct ggcgctcaac
     4021 ggagtcagcc ggaacaatgt gcggcgcatc agcaacgcgc cagagattac gggctacaga
     4081 catcgcagac tcctgataac atcgcccgcc agatgccaca ggccgtgacg acgtaaatga
     4141 tgccagaata actggcgcag cggtggaaca ggtttatcac cctgatccca taaatcgcgg
     4201 gaaatatcct gcattaacgc aggccacgta cggtaccatc ccgggcgttg caaaagcgcc
     4261 ggaacatgct gataacgctg tagaacatcc cacagcaggc tgcgctctac gttgtgatga
     4321 taaagcggaa acagattttg cggctcgcgg tgctggcagg cgcttatcag cgtttgtgcc
     4381 gccgcctgcg cgccagtcag cgccatatcc atgccgcgca cggaaattcc ggtattgacg
     4441 caactgcgca acgcatcgcc caccagcagc cagccgttac cggcgtattg caccggcata
     4501 ctgtgcaagc caccttctgg caccagatgc gcaccatact ccagtgattc cgtgtttttg
     4561 ataagcgggc gcactgccgg atgcgcttta aagcgagtca gcagctcgct tgccggaaca
     4621 cggctttgcg taagggaaga gagcgggcaa acaatcccta acgagagcgt ttgttgatta
     4681 gtataaagaa atgcgccgcc gggtaagtca tcacagatcc cgccgctgaa cagcaacgct
     4741 gcgccttcgt tattctccag atgaaaacgt tcttcaatag cggatgtttc cagcgacagc
     4801 acttctttga tccccaacgc catcgcttcg ccagcaggac gagtcactaa cccgtgacgc
     4861 tcagccagga cgctgttggc accttctgcc agcaccacat aacgggcgcg gagaatatcg
     4921 tcaccacaaa tgacaccaca caccctgccg ttttcttcat acagtgcatc caccgtcgca
     4981 ccagggatgc attcgacacc ttctttttcg gcttcggcaa ccagccacgg atcgaaccgt
     5041 gcacgtaata cactccagga ttcaccgccg ggctgtaagc tggaaaacgt cgttgcgcca
     5101 tccggcgtta acagggaaag gctttcgtga gtgatgcatc gttcaagagg cgcggtcaga
     5161 tgaaactgag ggaggagttc cgcgagtgca tgggtatata accgcccgcc ggaaagattt
     5221 ttgctgccgg ggatttcagc gcgttccagt aacaaaacgg ataaacccgc tcgcgcgcag
     5281 cgtaacgcgc aagcggtgcc tgcaataccg gcaccaataa taataatgtc gcagtcgtct
     5341 tccatgtcat gccccagata tccagaacac aacaccctaa catagcgtta cttaagggaa
     5401 attgaccgcc gacacactct tatcattcgc cgcgatagat acaaccagcg gtgcaggttt
     5461 cttttaccat caccgcactt aacagcggca caacgggttt aacctgatcc caaatccatt
     5521 ttgctaaaac ttcgctggtc gggttttcca ggcctggaat atcattgaga taatggtgat
     5581 cgaggcgctc ataagttggc ttaaacgctg ctttcagttc agcgaaatcg ataatccagc
     5641 ccgtatgcgg atcgacttcc ccggtaattt ccagtcgcac cataaaggaa tgcccgtgca
     5701 ggcgaccaca tttatgccct tccgggacgt gtggtaagcg gtgagcggct tcgaaggtga
     5761 aatctttaaa taacgtggtg gacatcataa tttctctaca ggacgataaa accgccgtag
     5821 agtaccggaa aggctgtttt ttcgcattat ctaaaacgac tttgttgcgc aaaatcgctg
     5881 atttatctta atgattggct aaattcattt gtttttcatt aggttggtta atctattttg
     5941 ttgttaaaga ctattgctaa aacaggttag tcgatttggt tattagttat cgctatcccg
     6001 tctttaatcc acaccgttca ccccgttaac cttaccttct cttctgtttt atgggcgctg
     6061 acagggcgca gaaacagctt tgcttactgg aacataacga cgcatgacga cacaggtccc
     6121 accttccgcg ttgcttccgt tgaacccgga gcaactggta cgccttcagg cggccacgac
     6181 cgatttaact cccacccagc ttgcctgggt ttctggctat ttctggggcg tgctcaatca
     6241 gcagcctgct gcgcttgcag cgacgccagc tcccgccgca gaaatgccgg gtataactat
     6301 tatctccgcc tcgcaaaccg gcaatgcgcg ccgtgttgct gaagcattac gtgatgattt
     6361 attagcggca aaactgaacg ttaagctggt gaacgcgggc gactataaat tcaaacaaat
     6421 cgccagcgaa aaactgctta tcgtggtgac gtcaacgcaa ggagaagggg aaccgccgga
     6481 agaagccgtc gcgctgcata agttcctgtt ctccaaaaaa gcgccgaagc tggaaaacac
     6541 cgcatttgcc gtgtttagcc tcggcgatag ctcttatgaa tttttctgcc agtccgggaa
     6601 agattttgac agcaagctgg cggaactggg tggtgaacgc ctgctcgacc gtgtcgatgc
     6661 cgatgttgaa taccaggctg ctgccagcga gtggcgcgcc cgcgtggttg atgcgcttaa
     6721 atcgcgtgcg cctgtcgcgg caccttcgca atccgtcgct actggcgcgg taaatgaaat
     6781 ccacaccagc ccgtacagca aagacgcgcc gctggtggct agcctctctg ttaaccagaa
     6841 aattaccggg cgtaactctg aaaaagacgt tcgccatatc gaaattgact taggtgactc
     6901 gggcctgcgt taccagccgg gtgacgcgct gggcgtctgg tatcagaacg atccggcact
     6961 ggtgaaagaa cttgtcgaac tgctgtggct gaaaggcgat gaacctgtca ccgtcgaggg
     7021 caaaacgttg cctctgaacg aagcgctaca gtggcacttc gaactgaccg tcaacaccgc
     7081 caacattgtt gagaattacg ccacgcttac ccgcagcgaa acactgctgc cgctggtggg
     7141 cgataaagcg aagttacagc attacgccgc gacgacgccg attgtcgaca tggtgcgttt
     7201 ctccccggca cagcttgatg ccgaagcgct aattaatctg ctgcgcccgc tgacgccgcg
     7261 tctctattcc attgcctcct cgcaggcgga agtcgagaac gaagtacacg tcaccgttgg
     7321 tgtggtgcgt tacgacgtgg aaggccgcgc ccgtgccggt ggtgcctcca gcttcctcgc
     7381 tgaccgcgtg gaagaagagg gcgaagtccg cgtatttatc gaacataacg ataacttccg
     7441 cctaccagcc aatccagaaa ccccggtgat tatgattggc ccaggcaccg gtattgcgcc
     7501 gttccgcgcc tttatgcagc aacgcgccgc cgacgaagcg ccaggtaaaa actggctgtt
     7561 ctttggtaat ccgcacttta cggaagactt cctgtatcag gtggagtggc agcgctacgt
     7621 caaagatggc gtgctgacac gtatcgatct tgcctggtcg cgcgaccaaa aagaaaaagt
     7681 ttacgtacaa gacaaactgc gcgaacaggg cgcggagctg tggcgctgga tcaatgatgg
     7741 tgcccacatt tatgtctgcg gcgacgctaa tcgcatggcg aaagacgttg agcaggcact
     7801 tctggaagtg attgccgaat ttggtggcat ggacaccgaa gcggcggatg aatttttaag
     7861 tgagctgcgc gtagagcgcc gttatcagcg agatgtctac taatgagcga aaaacatcca
     7921 gggcctttag tggtcgaagg aaaactgaca gacgccgagc gcatgaagct tgaaagcaac
     7981 tacctgcgcg gcaccattgc ggaagattta aacgacggtc tgaccggcgg ctttaagggc
     8041 gacaacttcc tgctgatccg cttccacggc atgtatcagc aggatgaccg cgacatccgc
     8101 gccgaacgtg ctgaacagaa gctggagccg cgccacgcga tgctgcttcg ctgtcgtctg
     8161 ccgggtgggg tgattaccac taaacagtgg caggcgatcg acaaatttgc cggtgaaaac
     8221 accatctatg gcagcattcg cctgaccaac cgccagacgt ttcagttcca cggcattctg
     8281 aaaaagaacg tcaaaccggt gcaccagatg ctgcactcgg tcggtcttga tgcgctggcg
     8341 acagctaacg acatgaaccg taacgtactc tgcacctcga acccttacga gtcgcagctg
     8401 cacgcggaag cgtacgagtg ggcgaagaag atttctgagc atctgttgcc tcgtacccgc
     8461 gcgtatgcgg agatctggct cgaccacgaa aaagtcgcca ctactgatga agaaccgatc
     8521 cttggtcaga cctacctgcc gcgtaagttc aaaaccacgg tagtgatccc gccgcagaac
     8581 gatatcgatc tgcatgccaa cgacatgaac ttcgtggcaa tcgccgaaaa cggcaagctg
     8641 gtaggcttca acctgctggt gggcggtggg ctttctatcg aacacggcaa caagaaaacc
     8701 tacgcccgca cggcgagcga gtttggctat ctgccgctgg agcatacgct ggcggtggcc
     8761 gaagccgtcg tgacaactca gcgtgactgg ggtaaccgaa ccgatcgtaa aaatgccaaa
     8821 accaaataca cgctggagcg cgtgggggtt gagacgttta aagcggaagt ggagcgtcgc
     8881 gcggggatca aatttgaacc gatccgtcca tatgagttca ccggacgagg cgatcgtatt
     8941 ggctgggtta agggcattga tgataactgg cacctgacgc tgtttatcga aaatggtcgc
     9001 atccttgatt atccggggcg tccgctgaaa accggcctgc tggagatcgc gaagatccac
     9061 aaaggcgatt tccgcattac ggcgaaccag aatctgatca tcgccggtgt accggaaagc
     9121 gagaaagcga agatcgagaa gatcgccaaa gagagcgggt taatgaatgc cgtcacgccg
     9181 cagcgtgaaa actcgatggc ctgcgtctcg ttcccgactt gcccgctggc gatggcggaa
     9241 gcagagcgtt tcctgccgtc ttttatcgac aacatcgata atttaatggc gaaacatggt
     9301 gtcagcgatg agcatatcgt gatgcgtgta acaggctgcc cgaacggttg tggtcgcgcg
     9361 atgctggcgg aagtgggcct agtgggtaaa gcgccgggtc gctacaacct gcatcttggc
     9421 ggcaaccgca ttgggacacg tatcccacgg atgtataaag aaaacatcac cgagccggaa
     9481 atcctggcgt cgcttgatga actgataggg cgctgggcga aagagcgcga agcgggtgaa
     9541 ggcttcggcg actttacggt gcgtgcgggc atcattcgcc cggtgctcga tccggcgcgc
     9601 gatttgtggg attaaccatc agcccggtct tgtaggcctg ataagacgcg tgagcgtcgc
     9661 atcaggcaag gcaaacagtg aggaatctat gtccaaactc gatctaaacg ccctgaacga
     9721 actgccgaag gtagatcgca ttctggcgct ggcagaaact aacgccgaac tggaaaaact
     9781 ggacgctgaa ggccgcgttg cctgggcgct ggataatctg cccggtgaat atgtactttc
     9841 ttccagcttt ggcattcagg cggcggtgag cctgcatctg gtgaatcaga ttcacccgga
     9901 tattccggtg atcctcaccg acaccggcta tctgttcccg gaaacctacc gctttattga
     9961 cgagttaacg gacaaactca agctcaacct gaaagtgtac cgcgctaccg aaagcgccgc
    10021 ctggcaggaa gcacgctacg gcaaactgtg ggaacagggc gttgaaggca ttgaaaagta
    10081 caatgacatc aacaaagtcg aaccgatgaa ccgggctctg aaagaactta acgcgcaaac
    10141 ctggtttgct ggcctgcgcc gtgaacaatc cggcagtcgc gccaatttac cggtgctggc
    10201 aattcagcgt ggcgtattta aagtgctgcc gattatcgac tgggataacc gaactattta
    10261 tcagtacctg caaaaacatg gcctgaaata tcacccatta tgggatgaag gatatttatc
    10321 ggtcggtgat acccatacaa cccgtaaatg ggaacctggc atgtcggaag aagaaacacg
    10381 tttctttggc ttaaaaaggg aatgtggatt gcacgaaggg taaatattgc cggataaatt
    10441 aaacgcctct gtcagaaatg atgggggcgt ttttattttg cgagcagtat tcctgatttt
    10501 aaacgtgaac gccgctggaa gaattatcag gtatagaatg gcgtcgggtg cttgaggctg
    10561 tctggcgtta agcattcgcg aggttccaga tggacaaaag ccccaggcga tatttctatc
    10621 aacctgaggc ttacgttcga acccaaacaa ttcgaatgtt agtctcttct ttgcagggag
    10681 gcaagacatg ctgacaaaat atgcccttgt ggcaatcata gtgctgtgtt ttacagtact
    10741 gggattcacg ctgatggtag gcgactcgtt gtgtgagttg agtatcagag aacgtggtat
    10801 ggagtttaag gcagttctcg cttacgaatc gaagaagtag ccaccacgcg ggggtaaaac
    10861 cccgcgtact gaattgtgat ggtttggtct cctgcaccct tttttaattt atattgttta
    10921 tgtgaatatt atgcctatgt ttattattca tatatattac ggatgaaaat tatttgatta
    10981 ttcataatgt aaatatttta ttgaatgata tatatcacag ataattttta atatatgcgt
    11041 aaatatcctt taagtttact gaaggataaa aatattgtga ctttttttga tttctgggga
    11101 aaaacccgac gcggcgagaa agacgggggc gacgactatc accttctttg ctggcattcg
    11161 ctggatgtag ccgcgatggg ctatttaatg gttaaaagaa attgcttcgg gctggctgat
    11221 tattttcgtc aattagggat ttctgacaag gaacaggcgg ctcaattttt cgcctggttg
    11281 ctgtgctggc acgatattgg aaaatttgcc cgctcttttc aacaacttta cctgccccct
    11341 gaactcaaga ttcaggaagg tgccagaaaa aattacgaaa aaatctctca ttcaacgctg
    11401 ggctactggt tgtggaatca ttatttaagt gaatgtcagg agttacttcc ttcatcttcg
    11461 ctctctccgc gcaaacttag gcgagtgata gaaatgtgga tgcccgtgac gacagggcat
    11521 catggtcggc cccctgatcg tatggatgaa ttggataatt ttctgcctga agacaaagct
    11581 gcagcacgag attttctcct tgaaatcaaa ccactgtttc cgcttataga gatccccgca
    11641 ttctgggatg atgatgaggg cattgaactt ataaaacacc tttcctggta tatctctgca
    11701 accgtcgtgc tcgcagactg gacgggttcg tcaacgcgat tttttccacg cgtcgcacac
    11761 ccaatggata ttaaaggtta ctggcagaaa actttaattc aggctcaaaa cgccttaacc
    11821 gtctttcctc taaaagcaaa agtcgcgcct ttcaacggaa ttaatacgct gtttcctttt
    11881 attgagaatc cgacaccgtt acagcaaaag gtgctggatc tggatatcag ccagcaagga
    11941 ccgcagttat ttattctgga agatgtgact ggcgcaggta aaacagaagc ggcgcttatc
    12001 ctggcgcaca ggttgatagc tgcggggaaa gctcaggggc tgttttttgg tctgccaaca
    12061 atggcaacgg ccaatgccat gtacgatcgg ctggtcaaaa cctggcttgc tttctattcg
    12121 ccagaatccc gccccagcct ggtgctggca catagtgccc gcacattaat ggaccgcttt
    12181 aatgaatcac tctggtctgg tgatttagtc gggtcagaag aaccggatga acaaacgttc
    12241 agtcagggat gtgcggcctg gtttgccgac agtaacaaga aggcgttgct ggctgaaatt
    12301 ggcgtcggca cgctggatca ggcgatgatg gcggtgatgc cgttcaaaca taataatctg
    12361 cggcttctgg ggctgagtaa caaaatcctg ctggctgatg agatccacgc ctgtgacgct
    12421 tacatgtcgt gcattcttga agggttgatc gagcggcagg cgcgtggcgg aaacagcgtt
    12481 attttgcttt ctgccacgtt atctcaacag cagcgcgaca aactcgtcgc tgcctttgcg
    12541 cggggtacag agggcctgca agaagctccg ttcctggaaa aggatgatta cccctggctg
    12601 acgcatgtca cgaaatccga tgtgaactca caccgggtag cgacgcgcaa agacgttgag
    12661 cgtagcgtca gcgtgggttg gcttcatagt gaacaagagt gtattgcgcg tatcgaatcg
    12721 gcggtaagtc aggggaaatg catcgcctgg atccggaatt ctgtcgatga cgctattaag
    12781 gttcatcgtc agctgcttgc ccgcggcgtc attcctgctt ccagcctttc actttttcat
    12841 agccgctttg cttttagcga tcgccagcga attgaaacgg agacactggc acgctttggt
    12901 aaatattgta gtttacagag agcgtcgcaa gtcattgttt gtacacaagt aattgagcaa
    12961 agcgtggata ttgacctgga cgaaatgatc tccgacctgg cccctgttga tttgctgatt
    13021 cagcgagcag gacgattaca gcggcatatc cgcgatatta atggtcagtt aaagcgtgac
    13081 ggaaaggacg agcgttcccc ccctgaattg ctgattctgg ctcccgtctg ggacgactct
    13141 cctggcgacg aatggtttgg cagtgccatg cgtaacagtg catttgtcta tcccgatcat
    13201 gggcgaatct ggctgacgca gcgtgtgctg cgtgagcaag gtgctattca gatgccacac
    13261 gcagcccggc ttcttattga atccgtctac ggtgaggacg tggtaatgcc ggaaggattt
    13321 gcccgcagcg agcaggagca agtgggcaaa tattactgtg atcgcgcaat ggctaaaaag
    13381 tttgtgctga acttcaggcc cggctatgcc gccaatatca acgattacct cccggaaaaa
    13441 ctgtcgacac gtctggctga ggagtctgtt tccctgtggc tggctacctg tattgacggt
    13501 gtggtgaagc cttatgccac cggtgctcac gcatgggaaa tgagcgttgt cagagtgcgc
    13561 cgaagctggt ggaaaaaaca tcgggatgag ttttctttac tggagggaga ggcgttcagg
    13621 ctgtggtgca ttgaacagcg acaagatccg gagatggcaa acgtgatttt agtcaacgat
    13681 gacgaaagtt gcgggtattc ggctacggaa ggattgattg gcaaggttgg ttgagatgtc
    13741 tgcgacagga ctctctgaat agttgataca ccgaaaagga tggaaagatg gacaaccgac
    13801 ctgggatggt cttttttacg agaggatgcg tatgaactcg ttttcacttc tgacaacccc
    13861 gtggttgcct gttcgtttta aagacggaac gacaggcaag ctggcaccag tcgatctggc
    13921 tgatgaaaat gttgtcgata tcgctgcgcc gcgggcagat ctccagggtg cggcctggca
    13981 gtttttgctg gggctactac aaaccagctt cgcgcccaaa aatcacggtc gttgggatga
    14041 tatctggcaa gacggactgg aggctgaaaa gctacgggaa gcattgctgt ccttagaaca
    14101 cgctttccag tttgggccag attccccttc atttatgcag gattttgatg aattaaaagt
    14161 taaggcaact tctattgctt cgcttcttcc tgacgcacca ggaaaacaaa caaaagaacg
    14221 aaatacagac cattttatta agcgagacac aactcagcat ctgtgcctgc attgtgtgcc
    14281 tttagcgctt ttttcaatcc agttgaatgc acctataggt ggacgcggtt actatcctgg
    14341 gctacgtggc ggagggcctc tgacgacact tatagaattg ctggagtatc agggcaatca
    14401 acaaacccct ttgtggcgaa agctgtggct aaacgtaatg ccgcaagatg aagccgattt
    14461 acctttacca aaaacctttg atgacctggt ctttccttgg cttgcaccga cgcgtaccag
    14521 cgaactggac ggtgctgtgg taacggatga acaagtcaat aaacttcagg cctactgggg
    14581 aatgccacga cgtatacgta ttgattttaa gacaacctca ataggtaact gcgacatttg
    14641 cggtaggcag agcgatgctc ttttgggctt gatgtcactc aaaaactatg gtgttcaata
    14701 cgtcatgtgg cgccatcctc taacacctta ccgtctgccg cttaaagagg gtggagattt
    14761 ttactcagtt aaaccacaac ctggtggttt aatctggcgc gactggttag gcctcataga
    14821 ggtaggtaac tcaaaaaaca atactgaact ccccgcacag gtcgtgaaac ttttgaatgc
    14881 cagcaatcta aagcagacaa gggtgggctt atggggattt ggttttgatt ttgaagatat
    14941 gaaggttcgt tgttggtatg aacaccattt cccgctgttg ttaaacaaaa aagaagacct
    15001 aatacctaag ttgcgtctgg cagcgcaagc ggcatcacac attctaagct tgttacaccg
    15061 agcgttgaaa gaagcatggt tctctgagaa gaaaaccaca aagcttgatt tcggttttgt
    15121 ggatattgac ttctggaaca aaactcagca tcgattcctg agattagtgc gcaaaattga
    15181 agaaggtcag gatccgtacg aattactcag taaatggcaa aaggaaatgt ggttattcgc
    15241 gcgtcaggat tttgacgatc gtgtctttac caatccatat gagcccgttg atttggaacg
    15301 cgtcatgacc gcacgcaaga aatattttac aacatcagcg gagaagcaaa atgctaacgc
    15361 cgccagggag aaaaagcagg aggctgctga atgagtattg taaaagatga gcataaagtt
    15421 gcgctgcgta aatggcatga agagttgcag gataaacgcg gcgagcgtgc cagtctgcgg
    15481 cgtagtacga cggttaatga tgtttgtctg acggatggat ttcgcctgtt tcttaagaac
    15541 agacaaataa agtggcagga tgaacccgag tggcgtatca ccgcactagc gctgatagca
    15601 gcagttagcg ccaatgttaa agctattgac gaacggcagc cttttgccgc gcaactggcg
    15661 gcggtaatga aaaaggggcg gtttactcgt ttgttggcag taaaaacgcc ggatgattta
    15721 ttgcggcagt tacgccgtgc ggtcaaatta ttaaacggtt ccgtcaatct tgactctctg
    15781 gcagaggggg tcttccgctg gtgccaggag agcgatgacc tgctcaatca tcaccgccgc
    15841 cagcagcgac caacagaatt tatccgtatc cgctgggcgc tggaatatta ccaggctggc
    15901 gacgccgata acgaacaaaa ttaataaagg actatctcat gacaacattt attcagcttc
    15961 atttgttaac cgcttaccct gctgccaatc ttaaccgtga tgattccggc tctccgaaaa
    16021 ctgtgattct gggtggagca acgcgtctgc gcgtttcctc gcaaagtctg aagcgtgctt
    16081 ggcgcacttc tgaacttttt gaacaggcgc tggcgggcca tattggtgtt cgtagcgggc
    16141 gtattgcgcg tgaggcggca actatcctga ttgagaaagg aatcgaagat aaaaaagcca
    16201 tcgaatgggc agtagagata gccgattatc ttgggaaagc caaaaaagac aaaaaacaaa
    16261 aaaacgacaa aaaaccaaaa gatccgctca ctagcgccga aactgaacag ctcgtccata
    16321 tcagcccggc agaatttgac gccgtaaaag cgctggccca tcaactggca gaagaaaagc
    16381 gcgcgccaaa agagaaagat ctcgctttgt tacgtaaaga ccgtatggcg gtagatatcg
    16441 ctatgtttgg tcgtatgctg gcgaaaaaac ccgagtttaa tgtagaagcc gcctgccagg
    16501 tcgcgcatgc gtttggtgtc agtgaaacga ttgtcgaaaa tgattttttc accgccgtag
    16561 atgatttacg ccaggcttct gaagatgccg gtgccggtca tgtagatgaa accggattcg
    16621 gttctgcgct gttctacacc tatatctgca ttgataaaga tctgctggta gaaaacctcg
    16681 gcggtgacga agcgttagct aatcagacca tacgcgcctt tacggaagcc gcgcttaaag
    16741 tctccccaac cggtaaacaa aacagctttg ccagccgggc ttatgcctcc tgggcgctgg
    16801 cagaaaaagg caccgatcaa ccgcgttctc tggcggcggc tttctatgaa cccattaatg
    16861 gcacacgtca gttagatgtg gcggtgcagc gtattacaac gcttcgcgaa aacatgaata
    16921 cggtctacga acagaagacc gaatgcgcaa gttttgacgt gatgaacaaa cagggaagca
    16981 tgaaggacgt gctggacttt atctgcgcgt aaggggcggc ttatgagcca atatctgatt
    17041 tttcagcttc atgggccgat ggcgtcctgg ggggtcgatg ctccaggtga agttcgtcat
    17101 acccatgaac ttccttcgcg ctcagcattg ctggggctac tggctgccgg ggtagggatt
    17161 cggcgtgatg ataccgaacg cttaaacgcg tttaatcgcc actattcact ggtggtttgc
    17221 gccagccgta acccgcgctg ggcacgggat tatcacacgg tccagatgcc aaaagaggtg
    17281 cgtaaagcac gttatttcag ccgtcgcgaa gagttgagcg accctgagct tttgagcgcg
    17341 attatctccc ggcgcgacta ctacaccgat gcctggtgga tggtggcggt ggcaacaaca
    17401 gccgatgcgc cttacagcct cgaacagttg caggacggtt tacgccatcc agtttttccg
    17461 ctttatctgg ggcgaaaaag tcatccgctg gcattacctc ttgcgccgtt actgctcgaa
    17521 ggcaacgcgt gtgatgcctt atgtaatgca taccaacagt atcaggatca tttccataaa
    17581 ctgaaagtct cactcccgaa acttcaggat gaatgctggt gggaagggaa acacgacggc
    17641 ctggtcgcga gcaaaatatt acgccgccgg gatgttcctt taaatcgtca gcagtggctg
    17701 tttggggaac gcaccgtcaa tcaggggccg tggctcagca aggaggaacc atgtacctct
    17761 caagaataac cttacatacc ggccagcttt cgcctgcgca gttgctgcat ctggtggatc
    17821 gcggtgaata tgtgatgcat cagtggctgt gggatctctt ccctggcggc aaagaaaggc
    17881 aatttcttta tcgtcgggaa gaactccagg gcgcgtttcg cttttttgta ctgtcgcagg
    17941 aacgcccggc ggaaagtgat acgttcacca tagaatgccg gtcatttgcg cccgagctgt
    18001 gtaccggaca acaactttgt ttcaacctgc gggcgaatcc aacaatctgt aaagcgggca
    18061 agcgccacga tctgctgatg gaggcgaaac ggcaggtgag ggggcaggcg gaaggaagtg
    18121 atgtctggtt gcatcaacaa caggcggcgc tggactggct ggcagcgcag ggggagagaa
    18181 gcggttttac actgctggat acctcggttg atgcctaccg acaacagcaa ctgcggcggg
    18241 aaaactcccg gcaactgatc cagttcagta gcgtggatta tacggggatg cttacggtca
    18301 ccgatcctgg gttatttgtg caacgcctga gccaggggta tggtaaaagc cgggcgttcg
    18361 gctgtggtct gatgctgatc aaacccggag cagaggcgtg acgtttgtac cactgagccc
    18421 gatcccatta aaagatcgca cctccatgat cttcctccag tacggtcaga tcgacgtact
    18481 ggacggcgct ttcgtgctga tcgacaaaac agggatccga acgcatattc cggtgggatc
    18541 ggtcgcctgc attatgctcg aaccaggaac gagagtttcc cacgcggcgg tgcatctggc
    18601 cgccacggtg ggaacactgc tggtctgggt cggtgaagcg ggcgttcgcg tttactcctc
    18661 cggacaaccc ggtggggcgc gggcagataa attactctac caggcaaagc tggctttaac
    18721 ggaagatcta cggttgaagg tggtgcgcaa aatgtatgaa ttacgttttc gtgaaccaca
    18781 gccagctcgc cgttcagtgg agcagttacg tggaattgag ggatctcgcg ttcgccagac
    18841 gtatgcatta ctggcgaaac aatatggtgt gaaatggaat ggtcgcaaat acgatcctaa
    18901 agactgggaa aaaggcgatg ttgtgaatcg ctgcatcagt gctgctacat catgtctgta
    18961 cggtatatct gaagcggcag tattagccgc gggatatgcg cccgctattg ggtttatcca
    19021 tagtggcaaa ccgctttcat ttgtttatga catagccgat atcattaaat ttgattcggt
    19081 tgtgccaaag gcatttgaaa tagcagcgag gcaacccgca gaacctgata aagaagtcag
    19141 attagcctgt cgcgatattt tccgtagcac taagttaacg ggcaaattaa taccgttaat
    19201 tgaggaagtc cttgccgcag gtgaaattga accgccacaa cctgcgccgg atatgttacc
    19261 gccagccatc cccgaacctg aaacgttggg cgatagcggt catcgaggac gcggctgatg
    19321 agcatggtcg tggttgttac agaaaatgtc ccgccgcgct tacgtggacg gctcgcaatc
    19381 tggctactgg aagtgcgtgc cggtgtgtat gttggtgata catcaaaacg tattcgggag
    19441 atgatctggc agcaaattac ccaactggct ggttgcggaa atgtggtgat ggcctgggcg
    19501 accaataccg agtcgggttt tgaatttcag acctggggag aaaacagacg tattccggtg
    19561 gatttggatg ggttacgttt ggtttctttt cttcctgttg ataatcaata agttatccgt
    19621 tctttaaaaa taaggaaatg ttttaattta gttggtagat tgttgatgcg gaataaattt
    19681 gtttaaaaac agttatgtat gcttagtgtg ttccccgcgc cagcggggat aaaccgacga
    19741 ccaaatgatc tacgcgtatt gccacctcgt gttccccgcg ccagcgggga taaaccgtta
    19801 acgtctggat cctgtgtgcc atggaccgtg tgttccccgc gccagcgggg ataaaccgac
    19861 tgataacgcc ggagccttct ttttcagcaa gtgttccccg cgccagcggg gataaaccga
    19921 ggttgacgtt gattttgttc gttatgttgc cgtgttcccc gcgccagcgg ggataaaccg
    19981 gcaatttgtt gtccgcgatc cggtacgcgc gtgtgttccc cgcgccagcg gggataaacc
    20041 gcggctatgg aatttatgga gaagtttggt tttgtgttcc ccgcgccagc ggggataaac
    20101 cgggcgcact ggatgcgatg atggatatca cttagaattc cccgcccctg cggtagaact
    20161 cccagctccc attttcaaac ccatcaagac gccttcgcca gctccttcac caacggtagc
    20221 attatccgca taacgtcacg gcagcgacgt tctattcttc caggaagtgc cttatcaata
    20281 tgctgttgat tatccagtct tacgtcatgc cagctatttc ccgcagggaa tgcggctgtt
    20341 tttgcgcgtt gctgataacc atccttattc ccaagattcc agttagtcgc ttccaccgaa
    20401 agtacagcaa tgcccgcttt gtcgaatatt tctgcgtcat tacaacaccc agtgcctttc
    20461 ggataatttt tattcaaacc cggattggtc gttgcggcta ttccgtgact gcgcgcaatt
    20521 gccagcgccc tgtcgcgcgt taatttcctt actgcttcag gggtttttac accgctgttg
    20581 aaatacaatt tatcgccaac aattaagtta tcgagattaa tcaccagcag cgtatttttc
    20641 ttttcggtgt cactcatccg cttgagtaaa ttctcagcgc ctaatttccc ttcctcttcg
    20701 ccgctggtcg ccacaaatcg aataccatac tcggtaggcg tatttttcag gcgttctgcc
    20761 aattccagca tgacacctaa acctgcggcg ttatcatcca ttccttgtaa cgtcagcccg
    20821 ccgagattgg catcggcgtc agcatcgctc agcggggcgt aagtatccag atgcgccata
    20881 atgatgatct gctgcggcgc tttgccttca tgagcggcaa tcaccgtact tcctgtcacg
    20941 ttatgccagt tcttgcgatt atcgcgggcg gtataaatat accgactatt aaatgtccga
    21001 atatcactgc gataacccat ttgctgaaac tgttggcgaa tataatcggc agataacatt
    21061 tctgctggag ttccggtcat gcgtcccgga aagaaagtag caatatgtcg tgcctgggta
    21121 ttagcaaaat cgccaggttt aggtgacgag gcgtgtacgg ggagaataaa gcatacgccg
    21181 agcgccaggg cagcggtacg gtggcgcaat gcggaaaaca tagtgagtcc ttaaatacca
    21241 tgcaaatttt tttaccgcca tagtatgaaa ctgccgctgc gctaaaacaa tttcaaatct
    21301 tcctaaatgc ccgaaatccg gtgccttaag cactttttga tattagcttt gccaaatcgt
    21361 tattccgtta aggaactact cattctaatt ggtaatttca ttcgttctct tacgctccct
    21421 atagtcgaaa catctgatgg caagaaaata gcggtattgc aaaggaacgg ttatggatca
    21481 aaaacgactt actcacctgc ggcaactgga ggcggaaagc atccacatta ttcgcgaggt
    21541 ggcggcagaa ttctcaaatc cggtgatgct ctactctatc ggtaaagatt ccagcgtcat
    21601 gctgcatttg gcgcgcaagg cgttttatcc aggtacgctg cctttcccct tgctgcatgt
    21661 cgataccggc tggaaattcc gcgagatgta tgagttccgc gatcgtacgg cgaaagccta
    21721 cggctgcgag ctgctggtgc ataaaaaccc ggaaggcgtg gcgatgggga ttaatccatt
    21781 cgtgcacggc agcgcgaaac ataccgatat tatgaagacc gaaggcctga agcaggcgct
    21841 gaataaatac ggttttgatg ccgccttcgg tggtgcgcgc cgcgacgaag agaaatcccg
    21901 tgctaaagag cgtatctact ctttccgtga tcgcttccat cgctgggacc cgaaaaatca
    21961 gcgcccggag ctgtggcaca actataacgg gcagattaac aaaggcgaaa gcatccgcgt
    22021 tttcccgctc tctaactgga ccgagcagga tatctggcaa tacatctggc tggaaaatat
    22081 cgacattgtg ccgctgtatc tcgctgcgga acgtccggtt ctggaacgcg acggtatgtt
    22141 gatgatgatt gatgacaacc gtatcgacct gcaaccgggc gaagtgatta aaaaacggat
    22201 ggtgcgtttc cgtacgctgg gctgctggcc gctgaccggt gcggtggagt caaatgcaca
    22261 aacactgccg gagatcatcg aagagatgct ggtttccacc accagtgaac gtcagggccg
    22321 cgtgattgac cgcgaccagg cggggtctat ggagctgaaa aaacgtcagg ggtattttta
    22381 agatgaacac cgcacttgca caacaaatcg ccaatgaagg cggcgtcgaa gcctggatga
    22441 ttgcgcaaca acataaaagc ctgctgcgtt ttctgacctg tggtagcgtc gatgacggca
    22501 aaagtaccct gattggtcgc ctgctgcacg atacccgcca aatctacgaa gatcagctct
    22561 catcgctgca taacgacagt aagcgtcacg gcacccaggg cgaaaaactg gatctggctc
    22621 tgctggtgga cggcctgcaa gctgagcgcg aacagggcat cactattgat gtggcctacc
    22681 gctatttctc taccgagaag cgtaaattta ttatcgccga cacccccggg cacgagcagt
    22741 atacccgcaa tatggcgacc ggcgcctcaa cgtgtgagct ggcgatctta ctgatcgatg
    22801 cccgtaaagg cgtgctcgat caaacccgtc gtcacagttt tatctccacg ctgctgggga
    22861 tcaaacatct ggtcgtagcg atcaacaaaa tggatctggt ggattacagt gaagagacat
    22921 tcacccgcat tcgtaaagac tacctgactt tcgccgaaca gttacctggc aatctggata
    22981 ttcgctttgt gccgctctct gcgctggaag gcgacaacgt ggcttcgcaa agtgaaagta
    23041 tgccgtggta cagcggtccg acactgctcg aagtgctgga aaccgtggag atccagcgag
    23101 tggtggatgc tcagccaatg cgcttcccgg tgcagtacgt taatcgcccg aatctcgatt
    23161 ttcgtggtta cgccggaacg ctggcatccg gtcgcgtgga agtcgggcaa cgtgtaaaag
    23221 tgctgccttc tggtgtggaa tcaaacgtcg cgcggatcgt gacttttgat ggtgatcgcg
    23281 aagaagcctt tgccggagaa gcgatcaccc tggtgctgac ggatgagatc gacatcagcc
    23341 gtggcgatct gctgctggcg gcagacgaag cgttaccggc ggtgcagagc gcgtcggtgg
    23401 atgtggtatg gatggcggaa cagccgcttt ctccagggca gagttacgac atcaaaattg
    23461 ccggtaagaa gacgcgcgcg cgtgttgatg gcattcgcta tcaggttgat attaataacc
    23521 ttacccagcg tgaagttgaa aacctgccac tgaatgggat cggcctcgtg gatctcactt
    23581 ttgacgagcc gctggtgtta gatcgttatc aacaaaatcc ggtgacgggt gggctgattt
    23641 ttatcgatcg cctgagcaat gtgaccgtgg gtgccggtat ggtgcacgag ccagttagcc
    23701 aggcaactgc tgcgccatct gaattcagtg cattcgaact ggaattgaat gctctggttc
    23761 gtcgccactt tccgcactgg ggcgcgcgcg atttgctggg ggataaataa tggcgctgca
    23821 tgacgaaaac gtcgtctggc atagccatcc ggtcactgtg caacaacgcg agctacacca
    23881 cggtcatcgt ggtgtagtgc tgtggtttac cggcctctcc gggtccggta aatcaacggt
    23941 cgccggggcg ctggaggagg cgttacataa actcggcgtc agtacgtatc tgctggatgg
    24001 cgataatgtt cgccacggat tatgcagcga tctcggtttt agcgatgccg atcgtaaaga
    24061 gaatatccgt cgcgtcggtg aagtggcgaa tttgatggtt gaagccggac tggtagtgct
    24121 gaccgcattt atctcgccac accgcgccga acgccagatg gttcgcgaac gcgtaggaga
    24181 agggcgcttt atcgaagtgt ttgtcgatac gccgctggcg atttgcgaag cccgcgatcc
    24241 aaaaggctta tataagaaag cgcgtgccgg tgaactgcgc aactttacgg gaatagattc
    24301 cgtttacgaa gcgcctgaat cggcagaaat tcatctcaat ggtgaacaat tagtaacaaa
    24361 tttggtacag caattattag atctgttgag acagaacgat attatcagat cctgagacac
    24421 caccgggttt ccatgcccgg ttcgtcaaag taacaggatc aaatatgcgt aatagccata
    24481 acattacact aacaaataac gacagcctta ctgaggatga agaaaccaca tggtcactgc
    24541 ctggtgccgt ggtcggtttt atctcctggt tatttgcgct ggcgatgccg atgttgattt
    24601 atggctctaa cacgctgttc ttctttatct acacctggcc tttctttctg gcgctgatgc
    24661 ccgtcgcggt agtggtgggg attgcgctgc attcattgat ggacggaaag ctacgctaca
    24721 gtattgtttt caccctggtg acagtaggca ttatgtttgg cgcactgttt atgtggctac
    24781 tgggctaatt tgtactttcc cgtctcctct gttcataatt caaaccgtaa ctaataatga
    24841 gattatgttc tgcacgccct gggtatacgt aacaatggac aaatgtggta catttgcccg
    24901 cgttgtcgcg gtatccccaa cagaggatgt agagtcgtct tcggatgcat gggatgatga
    24961 tgccgttttt cagggggcag gatgggtaaa ctaacgctgc tgttgctggc tattctggtc
    25021 tggctacagt attcgctgtg gttcggtaag aacggtatac atgactatac ccgcgtcaat
    25081 gatgatgtgg cggcacagca agctacaaac gcgaaactta aagcgcgaaa cgatcaactt
    25141 tttgccgaaa ttgacgatct caatggcggc caggaggcgc tcgaagagcg tgcgcgtaat
    25201 gaactcagca tgaccaggcc gggcgaaact ttttatcgtc tggtgcctga cgcgtcgaag
    25261 cgcgcacagt ctgcggggca aaacaatcga taaatcagcc cgggaattaa catggcaacc
    25321 actcatttgg atgtttgcgc cgtggttccg gcggccggat ttggccgtcg aatgcaaacg
    25381 gaatgtccta agcaatatct ctcaatcggt aatcaaacca ttcttgaaca ctcggtgcat
    25441 gcgctgctgg cgcatccccg ggtgaaacgt gtcgtcattg ccataagtcc tggcgatagc
    25501 cgttttgcac aacttcctct ggcgaatcat ccgcaaatca ccgttgtaga tggcggtgat
    25561 gagcgtgccg attccgtgct ggcaggtttg aaagccgctg gcgacgcgca gtgggtattg
    25621 gtgcatgacg ccgctcgtcc ttgtctgcat caggatgacc tcgcgcgatt gttggcgttg
    25681 agcgaaacca gccgcacggg agggatccta gccgcaccag tgcgcgatac gatgaaacgt
    25741 gccgaaccgg gcaaaaatgc cattgctcat accgttgatc gcaacggctt atggcacgcg
    25801 ctgacgccgc aatttttccc tcgtgagctg ttacatgact gtctgacgcg cgctctaaat
    25861 gaaggcgcga ctattaccga cgaagcctcg gcgctggaat attgcggatt ccatcctcag
    25921 ttggtcgaag gccgtgcgga taacattaaa gtcacgcgcc cggaagattt ggcactggcc
    25981 gagttttacc tcacccgaac catccatcag gagaatacat aatgcgaatt ggacacggtt
    26041 ttgacgtaca tgcctttggc ggtgaaggcc caattatcat tggtggcgta cgcattcctt
    26101 acgaaaaagg attgctggcg cattctgatg gcgacgtggc gctccatgcg ttgaccgatg
    26161 cattgcttgg cgcggcggcg ctgggggata tcggcaagct gttcccggat accgatccgg
    26221 catttaaagg tgccgacagc cgcgagctgc tacgcgaagc ctggcgtcgt attcaggcga
    26281 agggttatac cctgggcaac gtcgatgtca ctatcatcgc tcaggcaccg aagatgttgc
    26341 cgcacattcc acaaatgcgc gtgtttattg ccgaagatct cggctgccat atggatgatg
    26401 ttaacgtgaa agccactact acggaaaaac ttggatttac cggacgtggg gaagggattg
    26461 cctgtgaagc ggtggcgcta ctcattaagg caacaaaatg attgagtttg ataatctcac
    26521 ttacctccac ggtaaaccgc aaggcaccgg gctgctgaaa gccaatccgg aagactttgt
    26581 ggtggtggaa gatttgggct ttgagcctga tggtgaaggt gagcatattc tggttagaat
    26641 cctcaaaaac ggctgcaata cccgttttgt ggcggatgca ctggcgaaat tcctgaaaat
    26701 tcatgcccgt gaagtcagct tcgctgggca aaaagacaaa catgctgtta cggaacagtg
    26761 gttatgcgct cgcgtgccag gcaaggaaat gcccgatctg agcgcctttc aactggaagg
    26821 ctgccaggtg ctggagtatg cgcggcacaa gcgcaagctg cgtttaggcg cgctgaaagg
    26881 taacgccttt accctggttc tgcgcgaagt gagcaatcgc gatgacgttg aacaacgtct
    26941 gatcgatatt tgcgtaaaag gtgtaccgaa ctacttcggt gcccaacgtt ttgggattgg
    27001 cggtagcaac ttgcaggggg cgcagcgctg ggcgcaaacc aatactccgg tgcgcgatcg
    27061 caataaacgg agtttttggt tgtcggcagc ccgcagtgcg ttgtttaatc agattgttgc
    27121 tgagcgcctc aaaaaagcag acgttaatca agttgttgac ggcgatgcgc tacaattagc
    27181 cggacgtggt agctggtttg tcgcaaccac cgaagaactg gcggaattac agcgtcgcgt
    27241 caacgataaa gagctgatga taaccgccgc attgccgggc agtggcgaat ggggaactca
    27301 gcgtgaagcg ctggcattcg aacaagcagc tgtcgccgca gaaactgaat tacaagcttt
    27361 actggtgcgc gaaaaagttg aagccgcgcg cagagcgatg ctgctgtatc cgcaacaatt
    27421 aagctggaat tggtgggatg acgtcaccgt agagatccgt ttctggcttc cggcgggtag
    27481 ttttgcaacc agcgttgtca gggaacttat caacacaaca ggtgattatg cgcatattgc
    27541 tgagtaatga tgacggggta catgcacccg gtatacaaac gctggcgaaa gccttgcgtg
    27601 agtttgctga cgttcaggtg gtcgcccccg atcgtaaccg cagcggcgct tcaaattctc
    27661 tgacactgga atcctccctg cgcacgttta cctttgaaaa tggtgatatt gctgtgcaaa
    27721 tgggaacccc gaccgattgc gtctatcttg gcgtgaatgc tctgatgcgt ccgcgcccgg
    27781 acattgttgt gtccggaatt aacgccgggc cgaatctggg ggatgatgtt atttattccg
    27841 gtacggtagc cgccgcgatg gaaggccgtc atttaggttt tccggcgctt gccgtctcgc
    27901 ttgacgggca taaacattac gacactgccg cggcggtaac ctgttcaatt ttgcgcgcac
    27961 tgtgtaaaga gccgctgcgc accgggcgta ttcttaatat taacgttccg gatttaccct
    28021 tggatcaaat caaaggtatt cgcgtgacgc gctgcggtac acgacatccg gcagatcagg
    28081 tgatcccgca gcaagatccg cgcggcaata cgctgtactg gattggcccg ccgggcggta
    28141 aatgtgatgc cggtccgggg accgattttg ctgcggtaga tgagggctat gtctccatca
    28201 cgccgctgca tgtggattta actgcgcata gcgcgcaaga tgtggtttca gactggttaa
    28261 acagcgtggg agttggcacg caatggtaag cagacgcgta caagcacttc tggatcaatt
    28321 acgtgcgcaa ggtattcagg atgagcaggt gctgaatgca cttgccgccg tgccgcgtga
    28381 aaaattcgtt gatgaagcgt ttgaacaaaa agcctgggac aatatcgcct tgccgatagg
    28441 tcaggggcag acaatttcgc agccgtatat ggtggcgcga atgaccgaat tactcgagct
    28501 gacgccgcag tcgcgggtgc tggaaattgg cactggttcg ggatatcaaa cggcaatcct
    28561 ggcgcatctt gtccagcatg tttgctcggt cgaacggatt aaaggtttgc agtggcaggc
    28621 gcgtcgccgc ctgaaaaatc ttgatttaca taatgtttca acccgtcatg gcgatggatg
    28681 gcaaggttgg caggcacgcg cgccgtttga cgctatcatt gttacggcgg caccgccgga
    28741 aattccaact gcgctaatga cgcagctgga cgaaggcggg attctcgtct tacccgtagg
    28801 ggaggagcac cagtatttga aacgggtgcg tcgtcgggga ggcgaattta ttatcgatac
    28861 cgtggaggcc gtgcgctttg tccctttagt gaagggtgag ctggcttaaa acgtgaggaa
    28921 atacctggat ttttcctggt tattttgccg caggtcagcg tatcgtgaac atcttttcca
    28981 gtgttcagta gggtgccttg cacggtaatt atgtcactgg ttattaacca atttttcctg
    29041 ggggataaat gagcgcggga agcccaaaat tcaccgttcg ccgcattgcg gctttgtcac
    29101 tggtttcgct atggctggca ggctgttctg acacttcaaa tccaccggca ccggtcagct
    29161 ccgttaatgg caatgcgcct gcaaatacca attctggtat gttgattacg ccgccgccga
    29221 aaatggggac gacgtctaca gcccagcaac cgcaaattca gccggtacag cagccacaaa
    29281 ttcaggctac tcaacaaccg caaatccagc cagtgcagcc agtagctcag cagccggtac
    29341 agatggaaaa cggacgcatc gtctataacc gtcagtatgg gaacattccg aaaggcagtt
    29401 atagcggcag tacctatacc gtgaaaaaag gcgacacact tttctatatc gcctggatta
    29461 ctggcaacga tttccgtgac cttgctcagc gcaacaatat tcaggcacca tacgcgctga
    29521 acgttggtca gaccttgcag gtgggtaatg cttccggtac gccaatcact ggcggaaatg
    29581 ccattaccca ggccgacgca gcagagcaag gagttgtgat caagcctgca caaaattcca
    29641 ccgttgctgt tgcgtcgcaa ccgacaatta cgtattctga atcttcgggt gaacagagtg
    29701 ctaacaaaat gttgccgaac aacaagccag ctgcgaccac ggtcacagcg cctgtaacgg
    29761 taccaacagc aagcacaacc gagccaactg tcagcagtac atcaaccagt acgcctatct
    29821 ccacctggcg ctggccgact gagggcaaag tgatcgaaac ctttggcgct tctgaggggg
    29881 gcaacaaggg gattgatatc gcaggcagca aaggacaggc aattatcgcg accgcagatg
    29941 gccgcgttgt ttatgctggt aacgcgctgc gcggctacgg taatctgatt atcatcaaac
    30001 ataatgatga ttacctgagt gcctacgccc ataacgacac aatgctggtc cgggaacaac
    30061 aagaagtgaa ggcggggcaa aaaatagcaa ccatgggtag caccggaacc agttcaacac
    30121 gcttgcattt tgaaattcgt tacaagggga aatccgtaaa cccgctgcgt tatttgccgc
    30181 agcgataaat cggcggaacc aggcttttgc ttgaatgttc cgtcaaggga tcacgggtag
    30241 gagccacctt atgagtcaga atacgctgaa agttcatgat ttaaatgaag atgcggaatt
    30301 tgatgagaac ggagttgagg tttttgacga aaaggcctta gtagaagagg aacccagtga
    30361 taacgatttg gccgaagagg aactgttatc gcagggagcc acacagcgtg tgttggacgc
    30421 gactcagctt taccttggtg agattggtta ttcaccactg ttaacggccg aagaagaagt
    30481 ttattttgcg cgtcgcgcac tgcgtggaga tgtcgcctct cgccgccgga tgatcgagag
    30541 taacttgcgt ctggtggtaa aaattgcccg ccgttatggc aatcgtggtc tggcgttgct
    30601 ggaccttatc gaagagggca acctggggct gatccgcgcg gtagagaagt ttgacccgga
    30661 acgtggtttc cgcttctcaa catacgcaac ctggtggatt cgccagacga ttgaacgggc
    30721 gattatgaac caaacccgta ctattcgttt gccgattcac atcgtaaagg agctgaacgt
    30781 ttacctgcga accgcacgtg agttgtccca taagctggac catgaaccaa gtgcggaaga
    30841 gatcgcagag caactggata agccagttga tgacgtcagc cgtatgcttc gtcttaacga
    30901 gcgcattacc tcggtagaca ccccgctggg tggtgattcc gaaaaagcgt tgctggacat
    30961 cctggccgat gaaaaagaga acggtccgga agataccacg caagatgacg atatgaagca
    31021 gagcatcgtc aaatggctgt tcgagctgaa cgccaaacag cgtgaagtgc tggcacgtcg
    31081 attcggtttg ctggggtacg aagcggcaac actggaagat gtaggtcgtg aaattggcct
    31141 cacccgtgaa cgtgttcgcc agattcaggt tgaaggcctg cgccgtttgc gcgaaatcct
    31201 gcaaacgcag gggctgaata tcgaagcgct gttccgcgag taagtaagca tctgtcagaa
    31261 aggccagtct caagcgaggc tggccttttt tctttcggtt ggtgagtcgg tgtatttgta
    31321 gtctggataa gacgcgatag cgtcgcatcc ggaaatttgc cccctatgcg ttcatcatct
    31381 tgcgcaccag ctgcataaac agctcctgct cttctccact caaacgcccc aaaaattcct
    31441 catccacgct gtcaccaatg ggtatcgccg cagcaagtat cttttcgcct tcggcagtca
    31501 accagacaaa gcgtcgccgc ttatcagcgg ggtcatgttc acgtctgact aagccacgat
    31561 tttccattct tgccaacatt tctgctaacg ttgctttggt gctgacagct gcttctatca
    31621 gtgccacctg ttcgatacca ggtttatcag caatggcgcg catcaccgcg tactgtggtt
    31681 tggtcaggtc aggtaactcg tgctgccagc gagccgtgtg ctgctgaaaa agctgtcgta
    31741 actgatggaa cgctttattt cgtaacgcca tgtaaactcc gggctaattc tctgtcgcta
    31801 tcatagcggt tttttagtga cgaagaagat gttgttactt ttcaatcctt gccgctgtgg
    31861 aaatgaagga gtatgttaat aataacgttc gtatacgaac aattaagagg ataagcaatg
    31921 aaactgatcg tcgggatgac aggggctacc ggtgcgcctc ttggtgtggc attactgcaa
    31981 gcgctgcggg agatgccgaa tgtcgagact catctggtga tgtcgaagtg ggcgaaaacc
    32041 accattgaac tggaaacgcc ttacagcgct cgcgatgttg ctgccctcgc agacttcagc
    32101 cataacccgg cggatcaggc ggcgatcatc tcatccggtt cttttcgtac cgacggcatg
    32161 atcgttattc cgtgcagtat gaaaacgctc gccggtatcc gcgctggtta cgccgatggc
    32221 ctggtagggc gcgcggcgga cgtcgtgctc aaagaaggcc gcaaactggt gctggtgccg
    32281 cgtgaaatgc cgcttagcac catccatctc gaaaatatgc tcgcactttc acgcatgggc
    32341 gtggcgatgg tgccgccgat gcctgccttt tataaccatc ccgaaacggt agatgacatt
    32401 gtccaccatg tggtagcccg cgtgctggat caatttggcc tcgaacatcc ccacgccagg
    32461 cgctggcaag gattgccgca ggcccggaat ttttctcagg agaatgaata atggcatttg
    32521 atgatttacg cagcttttta caggcgcttg atgaccacgg ccagttactg aaaatcagcg
    32581 aagaagtgaa cgccgagccg gatctggcag cagcagctaa cgccaccggg cgtatcggcg
    32641 acggcgcgcc cgcgctgtgg tttgataata ttcgcggctt taccgatgcc cgcgtggcga
    32701 tgaacaccat cggttcctgg cagaaccacg cgatttccct cggcctgccg ccaaatgccc
    32761 cggttaaaaa gcagattgat gagtttatcc gccgctggga taacttcccg attgccccgg
    32821 agcgccgcgc caatccagcc tgggcgcaga acaccgttga tggcgacgag atcaacctgt
    32881 tcgatatcct gccgctgttt cgtttaaacg atggcgatgg cggtttctgt ctcgacaaag
    32941 cgtgcgtggt ttcccgcgat ccgctcgacc cggataactt cggcaagcag aacgtcggca
    33001 tctaccgcat ggaagtgaag ggcaagcgta agctcggcct gcaaccggtg ccgatgcacg
    33061 atatcgccct gcatctgcat aaagcagaag agcgcggtga agatctgccg attgcgatca
    33121 cgctcggtaa cgatccgatc atcacgctga tgggggccac gccgctgaaa tatgatcagt
    33181 ccgagtacga aatggcaggc gcgctgcgtg aaagcccgta cccgatcgcc accgccccgt
    33241 tgaccggttt tgatgtgccg tggggttcag aagtgatcct cgaaggggtc atcgaaagcc
    33301 gtaaacgcga aatcgaaggg ccgttcggtg agtttaccgg gcactactcc ggcgggcgta
    33361 acatgaccgt ggtgcgcatc gataaagtct cttaccgcac caggccgatt ttcgaatcgc
    33421 tgtacctcgg tatgccgtgg accgaaatcg actacctgat ggggccagcc acctgcgtgc
    33481 cgctgtatca gcagctgaaa gccgagttcc ctgaagtgca ggcggtaaac gccatgtaca
    33541 cccatggcct gctggcgatt atctccacca aaaaacgcta cggcggcttt gcccgcgcgg
    33601 tgggcctgcg cgcaatgacc acgccgcatg gtctgggcta cgtgaagatg gtgattatgg
    33661 tcgatgaaga cgttgacccg ttcaacctgc cgcaggtgat gtgggcgctc tcctcgaaag
    33721 tgaacccggc aggggatttg gtgcagttgc cgaatatgtc cgtgctggaa ctcgatccag
    33781 gctcaagccc tgcggggatc accgacaagc tgattatcga cgccactacg cctgtcgccc
    33841 cggacaaccg tggtcactac agccaaccgg tggtggattt accggaaacc aaagcctggg
    33901 ctgaaaaact gaccgctatg ctggctgcac gtaaataagg agaagaagat gatttgtcca
    33961 cgttgtgccg atgaacagat tgaagtgatg gcgaaatcgc cggtgaaaga tgtctggacg
    34021 gtatatcagt gccagcattg cctttatacc tggcgcgata ccgaaccgct gcgccgtacc
    34081 agccgcgaac attatcccga agcgttccgc atgacgcaga aagatattga tgacgcgcca
    34141 atggtgccga gcatcccgcc gctgctggtg gaaggtaagc gttaaataaa aggtccgatg
    34201 cccatcggac ctttttatta aggtcaaatt accgcccata cgcaccaggt aattaagaat
    34261 ccggtaaaac cgagaatggt cgttaacact gtccaggttt tcagaccgtc tgctacagac
    34321 aaccccagat atttggtcac aatccagaac cctgagtcat taatatgtga cgcgccaagc
    34381 ccaccaaagc aggctgccag cgtcaccaat acgcactgaa tcggattcaa tcccatcacc
    34441 gcttctgaga gtaacccgcc ggttgtcagg attgctacgg ttgctgaccc ctgcgatgca
    34501 cgcagagcca gtgaaataat aaatgcggct ggtaacagag gcaggtcaat catttgtagc
    34561 atattggcaa gggctttgcc gacgcccgat tccaccagca ctttgccaaa tacccctcca
    34621 gcaccagtaa ccaaaatcac taccgccgca gtgggaaggg ctgagcccat aatgtcgctg
    34681 gtgtgttgta agctccagcc gcgacgtaaa gccaataacc agaatgccag caccagcgca
    34741 atcattagag ctaccattgg tgagccgatc agctgtagcg taccaagcag gggatgcgaa
    34801 ggcggcatca gtgttgcgga aaccgtaccc gccatgataa tcgcgatagg aataacaatt
    34861 agcgaggtga ccagcgcgac gcccggtgga tttattttat cgcttaattt tgttgcgcct
    34921 tcctcactgg ccggagccag ttgcatctgt tccagtactt ccaccgacat cgcatattgg
    34981 cgcgtattga ttattttcgc tgcaaagtag ccaacaatcc ctacgggaat agaaatcgca
    35041 ataccgatga tggttagcca gccgatgtct gcgtggagta accccgctgc ggcgacaggg
    35101 cctggatgcg gcggtaccgc cacatgaaca gttagcatga ttccagcgac aggcaggcca
    35161 aatttgagtg gcgatatttt ggcaaccttg gcaaaaccgt aaatgattgg cgcaagaata
    35221 ataaagccga catcaaagaa gacgggaata ccgaggaaga acgctgccag agtcagcgca
    35281 gcgatagttc gtttgtcacc taacttgcga ctgaaataat tagccagtga ctctgcacca
    35341 ccagagtgtt cgatcatacg ccccagcata gcgcccagac caataataat agtgacggaa
    35401 ccaagcacac cgcccatccc ggcgatcatc actttaccca cttcgcccgc cggtataccc
    35461 gccgcaagtg cgactaacag gctgacgagg agcagagcaa cgaatggttg tacctttgcc
    35521 ttgatgacca gcagcaacag catgattacg ccagctaacg caatgcataa caatgtaatt
    35581 gtggacatgg gaaaccctgt ctgaaagtta tagttaacct accccatccg tagatggggg
    35641 gatgtatggg tacgttgtaa ttagggattt aacgaattag cgccaggcgt caaaccagcc
    35701 aagcccttct tcggtgaggc cacgaggttt atattcacaa ccgatccagc cctgatatcc
    35761 cacctcatcg aacaggcgga acagccacgg atagttgatt tctccatcgt ccggttcatg
    35821 tcgatcaggt agtccggcaa tttgtacgtg cgcatatttc ccagcgtagt cgcggattaa
    35881 atgcgtcagg ttgccatcta ctttttgcgc atgaaaagta tctagttgaa taaacacgtt
    35941 atctcgcgca acctcttcaa caatagccag tgcctgatac tggctggaga agagataatg
    36001 aggcttaacg tcggggctga gtgcttcaac taatattcgc ttgccgtgta gcgcaaagcg
    36061 gtcggcagcg tagcggagat tatcgataaa tactgcccgg taccgttcag cgtcttcgcc
    36121 agcgggcacg acgcctgcca tcacatggac ttgttcacaa ttgagcgcca atgcatattc
    36181 cagtgccagg tcgatgtctg cgtgtgcttc gtgctcacgt ccgggaaggg cggataatcc
    36241 ccattccccc gcattaatat ctccgggagc ggtattgaac agcgccagtg tcagatggtt
    36301 ttgctccagt tgcttttgga tttgcagggt ggagtagtca tagggaaaca gaaattccac
    36361 agcatcgaac ccggcttttc gcgctgcggc gaagcgttca ataaaaggca cttcggtgaa
    36421 catcatggat aaattagctg caaaacgagg cattgcatta actccttaat tccgcaattt
    36481 cacctgcggt cagataacgg atcgggcggt caccgagaat aaaaatcagc tttgccgttt
    36541 cctccagctc ttccatattg ttggcggctt cttgcaggct ttcaccgcaa accactgggc
    36601 catgatttgc cagtaaaaaa gcctgattgt ctgctgccag ttccgccaga tcctgtgcga
    36661 tgcgtttatc gcccggtcgg taataaggca ccagcgggac atttcccatc cgcatcacca
    36721 cgtatggtgt gaacggacga ataacgttgc tgctgtccag cccttgcagg caggaaagcg
    36781 ccgtcgacca tgtgctgtgc aaatgcacca ccgctttaca gcgcggattg ttgcgataca
    36841 gcgccagatg aaagagcacc tctttcgagg gtttgtcacc acttaaccat tcgccatccg
    36901 cggcgacttt ggaaagccgc tgcggatcga gattgcccag gcatgaacct gtcggtgtcg
    36961 ccagtaaatt cccgtcaggt aaaagcagcg acagattgcc agccgaaccg gttgcatagc
    37021 cgcgctgaaa gaatgaactg gcaatccgcg tcatctcctc tcgcaaagac tgctctactt
    37081 ttgcgaaatc gctcatgata aaaactctct ttgggctcgt gaaaaaaagg cttcatcacc
    37141 gaagttgcca gatttaaggg cgagtgagac aggcttatcc agtgcgttta cccacggcac
    37201 gccgggggaa atggttgggc caatatgaaa cccttttatg cccaggctct gtgtgactac
    37261 gccggaggtc tcaccgcctg cgacaataaa gcgtgtcacg ccttccgctg ctaaccgcgc
    37321 cgctagttga gaaaatagag tttctactgc ctgactggct ttttgtgcac cgtattgctg
    37381 ttgaattgct gccaatgcgt cagtgctggc ggtggcaaaa accagtggag caagtacact
    37441 ttcctggccc agaacccact ctgccagttc gtgtgcataa gcggccagag tttcggttga
    37501 gaggcagcgt gccacatcaa cttcacgggc tggtgcaatt tgacggtaat gtgccacctg
    37561 gcggttggtc atttgagagc atgaaccgga gagcactacg ccgcgcccag cgagcggatg
    37621 ccctgcttcg cgagcctggt taccgttttc ttgcgcccac tgccgggcca ggccaatcgc
    37681 cagaccagaa ccgcccgtta ccagtggggc atcgcgcaag gcttctccct gaatttccag
    37741 atggtgttcg gtcagcgcat caagcaccgc gtagcggtag ccctcttgct gtaagcgagc
    37801 cagctcttga cgaacggcat ccacaccttg ttcgaaaaca tgtgccgaaa cgacgccgca
    37861 gcgccctgtg gattgcgctt caaccagacg gggaagatag ctgtcggtca tgggatttac
    37921 cgggtgatgg cgcatcccgg attcggccag cagttgattc attacgaaca aatacccctg
    37981 ataaaccgta cgtccgttga ccggcagggc cggagagaag accgtaaacg gcgtgtcgag
    38041 agcatccatt aatgcatcgg taaccgggcc gatattacct ttcgccgtac tgtcgaaagt
    38101 agagcagtat ttgaaataga tctgtttgca accttgctgt tgcaaccagc tcagagccgc
    38161 cagcgattgc tgtgtggctt caaccaccgg acaggagcgc gttttcaggc tgatcaccag
    38221 tgcgtcgatt gcttccggca ttttacctgt tggaacaccg ttaatttgta ccgttggtag
    38281 accgttttcc accagaaaac tggcgatatc cgtcgcgccg gtaaaatcat cggcgataac
    38341 gccaatcttg atcatgattt cgctcccggt agagtgatgc cagagaaaat cttgataact
    38401 gcgctatcgt cttctttccc gtaacctgcg ttactggcgc tggtgaacat attcaatgct
    38461 gttgaggcca atggcagcgg gaagtgcagg gctttggctg tatcggcaac cagaccaaga
    38521 tccttaacaa aaatatcgac ggctgaatgc ggggtgtaat cgccatccac cacatgacgc
    38581 atccggtttt cgaacatcca ggaatttccg gcggcattgg tcacgacgtc atacatcaca
    38641 tccagcggga tccccgcacg ggctgcaagt gccatcgctt cggctccggc agcaatatgt
    38701 acgcccgcta acaactggtg aataattttt acggtcgaac ctagtcccgg ttctgcacct
    38761 atgcgataaa cttttccggc aacggcttcc agcacgggtg ccagtcgttc aaaggcaata
    38821 tcgctaccgg aggccatgac agtcatttca ccgttagcgg cttttactgc accaccagaa
    38881 actggcgcat ccagcatttc cagatcgaat ccagccagag cggtagcaat ttcttgcgca
    38941 tcagcactag cgatagtgga agaaaccatt actgccgtac cgggtttcag atgttgtgca
    39001 acgcctgttt caccaaacag cacctgttta acctgggccg cattgaccac cagcaccagc
    39061 agagcgtcca gtttttcggc aaacgtcgcg gcgttatcag aaaccccgca agcacctgcc
    39121 tctttcaacg tagcgcaggc attgctgttc aggtctgcgc cccaggtaga aagacctgcg
    39181 cggacacatg acagtgctgc tcccattccc attgacccta agccaacgat accgacatga
    39241 aactcagatc ccgttttcat ctgctctcct tgttaattta agtgatattt tgtttgatat
    39301 tgtgaatata agcgctggaa gataacgata tggtgagctg attcacataa attaacattg
    39361 tgtgttattt tatgtgaact aagcgttagt tgcgccgcgc acgtttcgca ggcaaatagc
    39421 gtagaatgtc agcaggacaa aggaaggagc aaaagttgat acccgtagag cgtcgccaaa
    39481 tcatccttga gatggtagct gaaaaaggca ttgtcagtat tgctgaacta acggacagaa
    39541 tgaatgtgtc acatatgacc attcgtcggg atttacaaaa actggagcag cagggagccg
    39601 ttgtgctggt gtccggaggc gtccagtctc cgggacgcgt ggcgcatgaa ccttctcatc
    39661 aggtaaaaac tgcgctggca atgacgcaaa aagcggctat tggcaagctg gcggcaagtc
    39721 ttgttcagcc gggaagttgt atctatctgg atgcgggaac gaccacgtta gcgatagcac
    39781 agcatctgat tcacatggag tcactgactg tggtcacaaa cgatttcgtt attgcgaact
    39841 acttgctcga caacagtaat tgcacaatta ttcacactgg cggtgcagtg tgtcgggaaa
    39901 accgttcctg tgtcggggaa gccgctgcga ccatgctgcg cagcctgatg attgatcagg
    39961 cttttatttc tgcatcgtca tggagtgtgc gggggatttc tacgccagcg gaagataaag
    40021 tcacggtgaa acgggcgatt gccagtgcca gccgccagcg agttttggtc tgtgatgcga
    40081 cgaaatatgg tcaggtggcg acatggctgg cgttaccatt aagcgagttt gatcagatta
    40141 tcacagacga cggtttgccg gagagtgcca gtctcgcgct ggcgaagcag gatctctctt
    40201 tgctggttgc gaaaaatgaa taatggcctg caataacatt tggttactca tgcttcacag
    40261 aagaagcatg agactacttt attttataaa atgacagccg cccgcttttc ggcgtgccgg
    40321 tatcaatata aatctggtta gcgaacgtct gaatgttatc aaacatcata tgtccaaata
    40381 taaaataatc agcgccgttt atttgttgta actcgccatt aagcgatttc tgcacacgat
    40441 caacaggcca gagtaattcg ctctccgcta tttctttacc aaagagatat tcactccccg
    40501 gataatctgc atgtgcgatg acatatttta tgttgtcgtt agtgatttca ataatatgtg
    40561 gaaggtgatg gaatttcagc aacagatcta ttgcctcttg ttgctctgaa tcatttaaat
    40621 cgaaaaacca gtcaccaccg ctggcaagcc acatatttcc atcgccagtt tcgaatgcat
    40681 ccaacgccat cgcttcgtgg ttgcctttaa ccgacgtaaa ccagggttgg tttagcaggc
    40741 gcagcacgtt aagactcttc ggcccacgat caatattatc gcctgtggaa ataagtaagt
    40801 cggtttcagg gtaaaaagag agttgatgta aacgggattg taataactga tattcaccat
    40861 gaatatcacc aacgacccat atatggcgat agtgatgggc attgattttt tgatagcgtg
    40921 tagatggcat ggttttaccc tgtaaaataa gctttcctat tatacagggt atttttattt
    40981 gattcgtcag ttgtcgttaa tattcccgat agcaaaagac tatcgggaat tgttattaca
    41041 ccagactctt caagcgataa atccactcca gcgcctgacg cggagtcagt gaatccgggt
    41101 cgagattttc cagtgcttcg accgcaggcg aagtttcttc tggcactgac agcaaagaca
    41161 tctgcgtacc atccacttgc gtcgcggcgg cgttcggcga aatgctttcc agctcacgca
    41221 gtttttgccg tgcgcgctta ataacctctt tcggtacgcc cgccagagca gcaaccgcca
    41281 gaccgtagct tttgctcgcc gcgccgtcct gcacgctgtg cataaaggcg atagtgtcgc
    41341 tgtgctccag cgcatcgaga tgtacgttgg cgacgccttc cattttctcc ggtagctggg
    41401 tcagctcgaa atagtgggtg gcaaacagcg tcaatgcctt aatcttattc gccagatttt
    41461 ctgcgcacgc ccacgccagc gacagaccat cgtaggtgga cgttccacgc ccgatctcat
    41521 ccattaacac cagactgtat tcggtggcgt tatgtaaaat attggcggtt tcagtcatct
    41581 ccaccataaa ggttgagcgc ccggaagcca ggtcatctgc cgcgcctacg cgggtaaaga
    41641 tgcgatcgat aggtccaatc tcgacttttt gtgccggtac atagctgccg atgtaggcca
    41701 tcagcgcaat cagtgcggtc tggcgcatat aggtactttt accgcccatg ttcgggccgg
    41761 taataatcaa catacggcgc tgcggcgaca gattcagcgg gttagcgata aatggctcat
    41821 tcagtacttg ttcaactacc ggatggcgac cttcggtaat gcgaatgccc ggtttatcaa
    41881 tgaaggtcgg gcaggtgtag ttcagggtat aggcccgttc cgccaggtta accagcacgt
    41941 cgagttccgc cagcgcgctc gcgctctgtt gcaacgcttc cagatgcggc aacagcaggt
    42001 cgaacagctc ttcataaagc tgtttttcca gtgccagtgc tttgcctttt gaggtgagaa
    42061 ctttatcttc gtactctttt agctctggaa tgatgtagcg ctcggcgttt ttcagcgtct
    42121 ggcgacgcat gtagttgatg ggtgccagat ggctttgccc acggctgatt tgaatgtagt
    42181 agccgtgcac cgcattaaag ccaactttca gcgtgtccag gccggtacgt tcacgctcgc
    42241 ggacttccag acgctccaga taatcggtcg cgccgtcagc cagcgcgcgc cactcatcca
    42301 gctcttcgtt atagcccgat gcgataacac caccgtcgcg taccagcacc ggcggtgtgt
    42361 cgatgattgc tcgctccagc agatcgcgca gctcggcaaa ctcgcccatc ttctcacgta
    42421 gcgcctgtac cggtgcacta tcgacagttt ctaactgcgc acgcagctcc ggcagttgct
    42481 ggaaagcgtg gcgcatacgg gccagatcgc gtgggcgagc agttcgtaaa gccagacgtg
    42541 ccagaatacg ttccaggtcg ccgacctgac gcagtaccgg ctgcaactcg gcggtgaaat
    42601 cctgcaatgc gccaatagtt tgctggcgct caagcaacac gcgggtatcg cgcactggca
    42661 tatgcagcca gcgtttcagc atacgactac ccatcggcgt gacggtgcag tcgagcacag
    42721 aagccagcgt attttccgca ccgcccgcca ggttctgagt aatttccagg ttacgacgtg
    42781 tcgcggcatc cataatgatg ctgtcctgct cacgttccat ggtgatagaa cgaatatgcg
    42841 gcagggtcgt gcgttgggta tctttcgcat actgcaacag acaaccggca gcacaaagtc
    42901 cgcgcggcgc gttctcgacg ccaaaaccga ccagatcgcg ggtgccaaat tgcagattca
    42961 actgctggcg cgcggtgtcg atttcaaact cccacagcgg gcgacggcgc aggccgcgac
    43021 ggccttcaat cagcgacatc tcggcgaaat cttctgcata cagcagttcc gccggattag
    43081 tgcgttgcag ctctgccgcc atcgtttcgc ggtcggccgg ttcgctcagg cgaaaacgcc
    43141 cggagctgat atccagcgtc gcgtagccga aacctttgct gtcctgccag atagccgcca
    43201 gcaggttgtc ctgacgctcc tgtaacaggg cttcatcgct gatggtgcct ggcgtaacga
    43261 tacgcacaac tttgcgctca accggacctt tgctggtcgc cggatcgcca atttgttcgc
    43321 agatggcaac ggactctccc tgattcacca gtttggcgag atagttttcc accgcatggt
    43381 agggaatccc cgccatcggg atcggctctc ccgccgaagc accgcgtttg gtcagtgaaa
    43441 tatccagcag ttgcgacgcg cgttttgcgt cgtcataaaa cagttcataa aaatcaccca
    43501 tccggtaaaa cagcaggatc tcgggatgct gggctttcag cctgagatac tgctgcatca
    43561 tgggcgtatg ggcgtcgaaa ttttctattg cactcatggg gttatgtccg gttccctgat
    43621 attaaatggg gtgtgatggt ttttattttc gttgtaatca cataaggcgc acatgatacc
    43681 ggagttaatc atttcagggg aagaagcgaa cggtgagttc ttgatgctgg ttccgggaaa
    43741 gatgctgttt tgcagctttt gcgtgatgct tatgaagcaa gtacgcaaaa ttgcgtcggt
    43801 tggcgaacct ttcttcttta gagtcatagt gccctttaag attaagatgt aatataaata
    43861 catcttaatg aggtggaggc agcatgtccg gcaagcgtat ctctcgtgaa aaactgacga
    43921 ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca ggcgtcagcg gagccggagc
    43981 attacgaggc gttgtttgtt tacgcgcaaa agcggctgga taaatgtgtg ttcggcgaag
    44041 agaaaccagc ctgtaagcag tgtccggtgc actgttacca gcctgcaaag cgtgaagaga
    44101 tgaaacagat tatgcgttgg gccgggccga gaatgctgtg gcgtcatccg atcttaaccg
    44161 tgcgtcatct catcgatgac aagcgtccgg tgccagaact gccggaaaaa tatcgtccga
    44221 aaaaacctcg tgaatagtat gccggacagg cagatctgtc cggcaatttg cagttaaatc
    44281 aatgccgatt tatcaattcc cagccgcttc atccgtgaca gcagggtcgt gcgtttcagc
    44341 cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc cgttggtttc tttcagcacg
    44401 cgaacaatca actgatattc atcttcgccc tcctgggcga caaccgttgc ggcaggcggc
    44461 gtttcaggtt ccggtaaaac aatatctggc aatgacagct gcagcacgtt accgcgtgtt
    44521 agcaataccg cgcgctcaat gacgttttcc agttcgcgta cgttacccgg ccactccatg
    44581 ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga tattgcgccc cagacgacgg
    44641 gcaattttga aggtaaaggc tttcgccagc agcggaatat cttccggacg ctcgcgtagt
    44701 ggcggcaggt gaatcgggaa tacgttcagg cggtaataga gatcgctacg gaactcacgg
    44761 tcggcgacca tttttttcag atcgcggtta gtcgcggcga ttagacgcac gtccgtctga
    44821 atgattttgt tgctgccgag acgttcaaac tcctgttcct gcaatacacg cagcaacttc
    44881 ggctgtaact ccagtggcat atcgcccact tcgtcgagga acagggagct tttatccgcc
    44941 agttcaaaac gaccaatgcg ctgggcgctg gcaccggtaa aagccccacg ctcatgacca
    45001 aacagatcgc tttccagcaa tccggcaggc atcgccgcgc agttcatttt gaccatgcgg
    45061 cgattattac gcccactgag attatggatc gcacgggcaa tcagctcttt acccgtgcca
    45121 gtttcaccga ggatcagcac ggtactgtca ctttgcgcca ccatttcaac ttgtttaagc
    45181 acgctgtaca tggcttcgct gcggccaata atctcgccaa attcactatc aacattgttg
    45241 agctgctcgg tcagggcgag gttttcatca accagccgtt ctttcagacg atggatttcc
    45301 tgataggcga gggcgttatc gacagcgatt gccacacgtt cggcaatctg gcgcagtaaa
    45361 ttcagattgg tagtggtaaa cactttctct tcgcattgcg ccagtttcag cacgcccagc
    45421 atggtgtcgc cagacatcag cggtaacagg cacaaggttt gaatctggtt gccccaggtg
    45481 tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct cgtggagatt gatcagcagc
    45541 atctctttac ttttgaacac gcgttcggtg agggttccgg cttcatcgac ttcgctctgt
    45601 tcgtgggcgg gatgctgttt atcaagatag tgagtggagt agatgttgag tttgttttta
    45661 cggtggctgc gtaagacgat actgatatcg tcaatgtcga aatagtaatg gatttctttg
    45721 gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa gcaccgcgtt ggtgatggcg
    45781 accaggatgc ggaagttatc gcgttcccgg cataacaact catagtcgac attgttaacg
    45841 acgcggctct ggatttgttc ggtgacgaca gaaacgatct gcgtaaatgt ttgcagacga
    45901 ttgaactctt tttcgctcca ggggcgatcg tcataacgaa taaattcaca gccaccaaaa
    45961 atatgccctt ccgccgccag tggcatcagg caatagtgac caaattttgg gtatagccca
    46021 cctgcggcca gctgcggcca ggtttcacaa aattcttcgt aactgcaatg cagcgtatca
    46081 gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa cagtttcgtc ttcatattta
    46141 atgggggtgt ctttttcacg cgacgcgtaa taagacgcac gttgagtctg cgcttgccac
    46201 aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta ccagttgcga aagagcctca
    46261 cacagcgagg ccagatcggg ctgctgcaat agtgtccgag tgatgtcgaa caacccttgc
    46321 tgtccgagat cactcatcgg tgtatatgac attttgttct tccaggaaag caccgcaaag
    46381 cggtgcaaaa ggtcattgac taactatagg agatttagaa ttttattagc atatacgcgg
    46441 aagcggttcg gcgtgtggta aatcgagggt tcgtttcaca ccatacagac cggcaagacg
    46501 aacaccttta cgttccacca cttcaccaat cagcgccgca tctttcccca gtggatggga
    46561 atgtaacgct gccagcactt gctctgccgc gttgcgttca acggcgatca ccagtttgcc
    46621 ttcgttggca aagttcaggg cgtccagtcc cagcagttcg caaacgccgc gcacggcagg
    46681 tttaaccggc agcgccgctt ctgaaatttc aataccacaa ccgcaggctg ccgcgaactc
    46741 atgaaccacc gcgtttacac caccacgggt ggcatcacgc agcgctttca cgccgggaat
    46801 gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag tcgctgacca gttcgccatc
    46861 cagccccagc tgctcacgca ggttaaggat agtcgccccg tggtcgccga gcgtgccgct
    46921 caccagcaac atatcgcctg cggttagcgt ctgtgcgccc caatgaatat tcgccggaat
    46981 tgcgcccatg ccagcggtgt tgataaacag tttatctgcc gcgccgcgct gcaccacttt
    47041 agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg gtttctgcca tgctggttac
    47101 tacggctttc agtgtctcca tcgacaatcc ttcttcgagg ataaagccac aggagagata
    47161 gcgcggaata gcgccactga ccgcaacgtc attcgcggtg ccgcaaatcg ccagcttgcc
    47221 gatattaccg ccaggaaaga acagcgggtc aatgacgtaa ctgtcggtgg agaacgccag
    47281 acgatcgcct tctgctacca gctgcgccag atcaagacgt gcctggtctt cctgctctgc
    47341 cagccacggg ttggcaaagg cttccataaa caggctgttg attaattgct gcatcgcctg
    47401 gccgccgctg ccgtgggcga gttggatatt attcacgctt cattctcctg ctgacgatac
    47461 tgataccacg cggcgcacgc tccttcggag gagaccatca gtgcaccaaa tgcggtttga
    47521 ggattacagg tgttaccaaa cagcgggcat tgatgcggct tacatttgcc cgttaatacc
    47581 tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg gtgccgggcg gaaatgtgct
    47641 tcggcatcga atcgttgata atccggcgtc aggtgcacgc cagaagattc aatcacgcct
    47701 aagccgcgcc attcgctgtc gccgttgaca cagaacacat cggcaatcgc ctgttgcgcc
    47761 agcaggttac cggcatccgg taccacccga cgatactgat tctctacctt gctgtgggcc
    47821 gctattttct gctccaccag catgaccacg ccttgcagta gatcaagggg ttcgaaacca
    47881 gcgaccacca gcggacgatg aaaatcgctg gcgataaaat tataggcatc agtgccgata
    47941 accatactaa cgtggcccgg cgcgaggaac gcgtcgatac cgttatccgg ctgttccagc
    48001 aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga agaagtaaaa attctgcaca
    48061 tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg gcatggtggt ttcaaaacct
    48121 aagccgaaga acaccacttt gcgggttgga ttctcctgcg ccagtttcaa cgcatccatc
    48181 ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg cctgcaacag cgatccctgt
    48241 ttccctggta cgcgcatggc gtcgccaaag gtacagaaga tgacttccgg atggctggca
    48301 atctccacgc aggtgtcgat tctacccatc ggcagtacgc acaccgggca acccggaccg
    48361 tggataaact caacgttttc aggcagtaac tggtcgaggc cgaatttaaa aatagcgtgg
    48421 gtatgaccac cgcacacttc cataatccgc agagggcgtt cggcggtgta agagagatgt
    48481 gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct gttccggcgc gcgatattca
    48541 tcaacaaaac gcattatttt tcctcgccat acaacagcgc gccgacatcc ggctcaacgt
    48601 caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc ggcttcatta attacgctca
    48661 tggcaaagcc aacgtgtacc agtacccact ggcccacgcg cggctgaccg ttttcatcgc
    48721 agctgccgac taacgttaaa tcgacatcgc gctgaatgcc gcagacgtcg acttttgcct
    48781 ggttgccgtc aatggtgcgg atctggccgg gaacgcctat gcacatcgct gtgtctccag
    48841 ccagttcagc cactggtcca tcccttcacc gctggtggcg gaaataagga tgatttcaat
    48901 ttctggattg acttcgcggg cgcaggcgat gcacttctca acgtcaaagt tgagatacgg
    48961 caacaggtca actttgttga gcagcatcag cgaggcggcg gcaaacatat gcggatattt
    49021 cagcggtttg tcttcacctt cggtaacgga aagcaccgcc actttgtgtt tttcaccgag
    49081 atcgaagctg gccgggcata cgaggttgcc aacgttttcg ataaacagaa taccgttatc
    49141 gtccagtggc agacgcggtg cggcgtcggc aatcatctgt gcgtcaagat ggcagccttt
    49201 acctgtgttc acctgaatcg ctggtgtgcc ggtagcgcga atgcgtgcgg catcgttcac
    49261 ggtttgctgg tcaccttcaa taaccgcgca cggaacgctg tctttcaggc gcattagggt
    49321 ttccgtcagc agggtggttt taccggaacc tgggctggaa accaggttga gcaccagttg
    49381 cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga cggttgtttt tgtccagcac
    49441 gtcaatttcg acttccagca tccgacgctg gctcatgccc ggtgcgtgag tgcccgcttc
    49501 gccatgaccg taatgcaggt cgccttcttc agtctggctg ggggtaaatt caggcgcttt
    49561 aatgccggtg attttcatct tcgggcgtgc cgccggggca aatggcgcgc tacgaaacgc
    49621 ggaatgaggg ttatgttcat caccctcgat atacaggttg ccttcaccgc aaccgcatgt
    49681 tgtacacatc gctcactcct ggtctatttc tatccgccga atctgtaaac cgtcgtctgc
    49741 cacaatctgt agcatgtcac catgacactg tggacagcgg cggacgcgct gggtcaatag
    49801 cgtcacatac tgttggcatg tttcacacca gcattcggcc tcttgttctt cgaggtgcag
    49861 tttacaacct tccgccacgc tgccgcggca aaccagatca aaacaaaagg caagagagct
    49921 ggtttcgaca caagaaaatg cgccaatttt gagccagacc ccagttacgc gttttgcgcc
    49981 gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt tggcagaggg ttatttcgtg
    50041 catatcgcct cccattaact attgccagct acaagcaata attgtgccag tgttgattat
    50101 ccctgcggtg aataatgtcg atgatgtcga aatgacacgt cgacacggcg acgaaattca
    50161 tctttagctt aaaaatctct ttaataacaa taaattaaaa gttggcacaa aaaatgctta
    50221 aagctggcat ctctgttaaa cgggtaacct gacaatgact atttgggaaa taagcgagaa
    50281 agccgattac atcgcacagc ggcatcgtcg cctacaggac cagtggcaca tctactgcaa
    50341 ttcgctggtt caggggatca cgttatcgaa agcgcgcctg catcacgcca tgagctgcgc
    50401 gccggacaaa gaactctgtt tcgtcctttt tgaacatttt cgtatttacg tcaccctggc
    50461 ggatggcttt aacagccaca ccatcgagta ttacgtcgaa acaaaagatg gcgaagacaa
    50521 acagcggatt gcgcaggcgc aactgagcat tgacggcatg attgatggca aggtcaacat
    50581 ccgcgatcgc gaacaggttc tggaacacta tctcgaaaaa atcgctggcg tttacgacag
    50641 cttatacacc gccattgaaa acaatgtgcc ggtgaattta agccaactgg taaagggaca
    50701 aagcccggca gcatgagctg aggctttgcc cgttttgcag gcgttacgcc tgtttgggga
    50761 tgggcgtgtc gatgagtgtc gaaaatgaca tttcatcggc atgttttcgt caaaaatgac
    50821 aatcacctga ggaatgcctg gtgaatcgtt ttgtaattgc tgactccacg ctctgtatcg
    50881 gctgccacac ttgtgaggcc gcctgttcag agacgcatcg ccagcacggc ctgcaatcaa
    50941 tgccgcgcct gagagtgatg ctaaatgaaa aagaatctgc gccgcagctc tgtcaccact
    51001 gtgaagatgc accctgcgcg gtggtctgcc cggttaacgc catcacccgc gttgatgggg
    51061 ccgtgcagtt gaatgaaagc ctgtgcgtaa gctgcaagct gtgcggcatc gcctgcccgt
    51121 ttggcgcaat tgaattttcc ggcagccgtc cgctggatat tcctgcaaat gccaataccc
    51181 cgaaagcgcc accggcaccg cctgctccgg cgcgtatcag tacgctgctt gactgggtgc
    51241 cgggtattcg cgcgatcgcc gtcaaatgtg acctttgcag ttttgatgaa caaggtccgg
    51301 cctgcgtgcg gatgtgcccg actaaagccc tgcatctggt ggataacacc gatatcgccc
    51361 gcgtcagcaa acgtaagcgt gagctgacct ttaacacgga ctttggcgat ctcaccttgt
    51421 ttcagcaggc tcaaagtgga gaggctaaat gagcgcaatt tccctgatca atagcggcgt
    51481 ggcatggttt gtcgccgccg ctgttctggc atttctcttt tcttttcaaa agacgctaag
    51541 tggctggata gccggaattg gcggcgcggt tggtagtctg tatacggcag ccgcgggctt
    51601 cactgtactg actggcgcgg ttggcgtgag cggtgcgctg tcgctggtaa gctacgatgt
    51661 gcaaatctct ccgcttaacg cgatttggct gattacgctc ggtctgtgcg gtctgtttgt
    51721 cagcctctac aacattgact ggcatcgcca cgcgcaggtg aagtgcaacg gcttgcagat
    51781 caatatgttg atggctgccg ccgtctgcgc cgtcatcgcc agcaacctcg gcatgttcgt
    51841 ggtaatggct gaagtcatgg ccttgtgtgc ggtgttcctt accagcaaca gcaaagaggg
    51901 caaactgtgg tttgcgctgg ggcgtcttgg cactctgctg ctggcgattg cttgctggct
    51961 gctgtggcag cgttatggca cgctggatct gcgcctgctg gatatgcgta tgcaacagct
    52021 gccgctcggt tccgatatct ggctgctcgg tgtgattggc tttggcctgc tggccgggat
    52081 tattccgctg cacggctggg tgccgcaggc acatgctaac gcctctgcgc cagctgccgc
    52141 gttgttttct acggtagtca tgaaaattgg cctgctgggc attttaaccc tgtcgctgct
    52201 gggtggtaac gcgccgctgt ggtgggggat cgcgctgctg gtgctcggca tgatcaccgc
    52261 gttcgtcggt ggtctgtatg cgttgatgga acacaacatc cagcgcctgc tggcttacca
    52321 caccctggaa aatatcggca ttatcctgct ggggctgggc gctggcgtaa cgggtatcgc
    52381 gctcgaacaa ccggcgctga ttgctcttgg cctggtcggt ggtctgtacc atctgcttaa
    52441 ccatagcctg ttcaaaagcg tactgttcct cggggcgggg agcgtctggt tccgtaccgg
    52501 tcatcgcgat atcgagaaac tcggtggtat tggcaagaaa atgccggtta tctccatcgc
    52561 catgttagtc gggttaatgg caatggctgc gctgccgccg ctgaacgggt ttgccgggga
    52621 atgggttatc tatcaatcct tcttcaaact gagcaatagt ggcgcgtttg ttgcccgtct
    52681 gctggggccg ctgctcgctg tgggtctggc aattaccggt gcgctggcgg tgatgtgtat
    52741 ggcgaaagtc tatggcgtca cgttcctcgg cgcgccgcgt accaaagaag ccgaaaacgc
    52801 cacctgtgcg ccgctcctga tgagcgtaag cgtagtggca ctggcgattt gctgcgtaat
    52861 tggcggtgtt gctgcgccgt ggctgctgcc gatgctctct gctgctgtac ctctgcctct
    52921 ggagcctgct aacaccaccg tttctcaacc catgatcaca ttgctgctga ttgcctgccc
    52981 gctgctgcca ttcatcatta tggcgatttg caaaggcgat cgtttgccgt cgcgttcccg
    53041 cggctcggcc tgggtgtgcg gctacgacca cgaaaaatca atggtgatta ccgctcacgg
    53101 ttttgccatg ccggtgaaac aggcgtttgc gccggtgctg aaactacgca aatggctgaa
    53161 tccggtgtct ctggtgccgg gctggcagtg cgaggggagt gcgttgctgt tccgccggat
    53221 ggcgctggtt gaactggcgg tgctggtggt gattattgtt tcacgaggag cctgagaatg
    53281 agtgttttat atccgttaat tcaggcgctg gtgctatttg ccgttgcgcc gctgctctcc
    53341 ggtattaccc gcgtggcgcg agcccgctta cataaccgtc gtgggccggg tgtgttgcag
    53401 gagtatcgtg acattatcaa actgctgggc cgccagagcg ttggcccgga tgcctccggc
    53461 tgggtgttcc gcctgacgcc gtatgtgatg gtgggcgtca tgctgactat cgctactgcg
    53521 ctgccggtgg tgaccgtcgg ttctccgctg ccgcaactgg gcgatttgat caccttactg
    53581 tatctctttg ccatcgcgcg tttcttcttt gccatttccg gtctggatac cggtagcccg
    53641 tttactgcta tcggcgcgag ccgtgaagcg atgcttggcg tgctggtcga accgatgctg
    53701 ctgcttggtc tgtgggttgc cgcacaggtt gccggttcca ccaacatcag caacatcacc
    53761 gacaccgttt atcactggcc gctgagccag agcatcccgc tggtgctggc gctttgtgcc
    53821 tgtgcgttcg ccacttttat cgaaatgggc aaactgccgt tcgacctggc ggaagccgag
    53881 caggagttgc aggaaggtcc gctctccgaa tacagcggta gcggctttgg cgtcatgaaa
    53941 tggggtatca gcctgaaaca gctggtggtg ttgcagatgt tcgtcggggt gtttattccg
    54001 tggggacaaa tggaaacctt caccgccggt ggactgctgc tggcgctggt gattgccatc
    54061 gtcaaactgg tggtcggcgt cctggttatc gcgctgttcg aaaacagcat ggcccgtctg
    54121 cgtcttgata ttactccgcg cattacctgg gctgggtttg gctttgcatt tttagcgttc
    54181 gtctccttgc tggcggcgtg attaaagaga gtttgagcat gtctgaagaa aaattaggtc
    54241 aacattatct cgccgcgctg aatgaggcat ttccgggcgt cgtgctggac cacgcctggc
    54301 agaccaaaga tcagctgact gtcaccgtaa aggtgaacta cctgccggaa gtggtggagt
    54361 ttctttacta caaacagggt ggctggctgt cggtgctgtt tggtaacgac gaacgcaaac
    54421 tgaatggtca ttacgccgtt tattacgtgc tgtcgatgga gaagggcact aagtgttggg
    54481 taacggttcg cgtcgaagtt gacgccaaca aaccggagta tccgtccgtg acgccgcgcg
    54541 ttccggcggc tgtgtggggc gagcgcgaag tgcgcgatat gtacggtttg attccggttg
    54601 gtctgccgga tgaacgccgt ctggtgctgc cggatgactg gccggatgaa ctttatccgc
    54661 tgcgtaaaga cagcatggat tatcgtcagc gtccggcgcc gaccaccgat gctgaaacct
    54721 acgagttcat caacgaactg ggcgacaaga aaaacaacgt cgtgccgatt ggtccgctgc
    54781 acgtcacttc tgacgaaccg ggccacttcc gtctgttcgt cgatggcgaa aacattatcg
    54841 acgccgacta ccgtctgttc tacgttcatc gcggcatgga aaaactggcg gaaacccgta
    54901 tgggttacaa cgaagtgacc ttcctctctg accgtgtgtg cgggatctgc ggctttgccc
    54961 acagcaccgc ctacaccacg tcggtagaaa acgcgatggg tattcaggtg ccagaacgtg
    55021 cgcagatgat ccgcgccatt ctgctggagg tggaacgcct gcactcgcat ctgctcaacc
    55081 tcggtctcgc ctgccacttt accggctttg actccggctt tatgcagttc ttccgcgtgc
    55141 gtgaaacctc catgaaaatg gcagagatcc tcaccggtgc gcgtaaaacc tacggcctga
    55201 acctgatcgg cgggattcgt cgcgatctgt tgaaagatga catgatccag acccgccagc
    55261 tggcgcaaca gatgcgtcgt gaagtgcagg agctggtgga tgtgctgctg agtacgccga
    55321 acatggaaca gcgcaccgtc ggcattggtc gtctggaccc ggaaatcgcc cgcgatttca
    55381 gtaacgttgg cccgatggtt cgcgccagcg gtcacgcccg cgatactcgc gccgatcacc
    55441 cgtttgtcgg ttatggcctg ctgccaatgg aagtccacag cgagcagggc tgcgacgtta
    55501 tttcccgtct gaaagtgcgt atcaacgaag tttataccgc gctgaacatg atcgactacg
    55561 gtctggataa cctgccgggc ggtccgctga tggtggaagg ctttacctac attccgcacc
    55621 gtttcgcgct gggctttgcc gaagcgccgc gcggcgacga tatccactgg agcatgaccg
    55681 gcgacaacca gaagctgtac cgctggcgct gccgtgccgc gacctacgcg aactggccga
    55741 ccctgcgcta catgctgcgc ggtaacaccg tttccgatgc gccgctgatt atcggtagcc
    55801 tggacccttg ctactcctgt accgaccgca tgaccgtggt cgatgtgcgt aagaaaaaga
    55861 gcaaagtggt gccgtacaaa gaactcgagc gttacagcat tgagcgtaaa aactcgccgc
    55921 tgaaataagg aatcgccatg tttaccttta tcaaaaaagt catcaaaacc ggcacggcga
    55981 cctcgtctta tccgctggag ccgattgcgg ttgataaaaa cttccgtggt aagccagagc
    56041 agaacccgca gcagtgcatc ggctgcgcgg cctgcgtcaa tgcctgcccg tcaaacgcct
    56101 taacggttga aactgacctc gccacaggag agcttgcgtg ggagtttaat ctcgggcgct
    56161 gcatcttctg tggacgctgc gaagaagtct gcccgacggc agcgatcaaa ctgtcgcaag
    56221 agtacgaact ggcggtgtgg aagaaagaag atttcctgca acagtcccgt tttgtgctgt
    56281 gcagctgtcg cgtctgcaat cgtcctttcg ccgtccagaa agagatcgac tacgccattg
    56341 cgctgcttaa gcacaatggt gacagccgcg cggaaaacca ccgcgaaagc tttgagactt
    56401 gcccggaatg taagcgccag aaatgcctgg tgccgtccga ccgtattgaa ctgactcgcc
    56461 atatgaaaga ggccatctga tgagcaattt attaggcccc cgtgacgcca acggcattcc
    56521 ggtcccgatg acggtggatg aatccatcgc cagcatgaag gcgtcgttac tgaaaaaaat
    56581 caaacgttct gcctacgttt accgcgtgga ctgcggcggc tgcaacggct gcgaaatcga
    56641 aattttcgcc acgctttcgc cgctgtttga tgcagaacgc ttcggcatta aagtcgttcc
    56701 ttcaccgcgt catgcggata ttttactgtt taccggcgcg gtcacccgtg caatgcgatc
    56761 ccctgcgctg cgtgcgtggc agtccgcgcc ggacccgaaa atctgtatct cctacggtgc
    56821 ctgcggtaac agtggcggga tcttccacga tctctactgc gtgtggggcg gtacggataa
    56881 aatcgtcccg gtagatgtct acatccctgg ttgcccgcca acgcctgccg ccacgctgta
    56941 cggctttgca atggcgctcg gcctgctgga gcagaaaatt cacgcccgtg gcccgggtga
    57001 gcaggatgaa caaccggcgg aaatcctgca tggcgatatg gtgcagccgc tgcgtgtgaa
    57061 agtggatcgc gaagcacgcc gcctggcagg ttatcgttac ggtcgtcaga ttgccgatga
    57121 cttcctgaca cagttagggc agggcgaaga acaggttgca cgctggctgg aagcggaaaa
    57181 cgatccgcgt ctgaacgaga ttgtcagcca tctgaatcat gttgtagaag aggcgcgtat
    57241 ccgatgagtg aaaaggtggt gttcagtcaa ctgagccgta aatttattga tgagaacgat
    57301 gccacgcccg ccgaggcgca gcaggtggtc tattacagcc tggcgattgg tcaccacctt
    57361 ggggttatcg attgcctgga agcggcgctc acctgcccgt gggatgaata tctggcatgg
    57421 attgccactc tggaggcggg cagcgaagcc cgccgcaaaa tggaaggcgt gccgaaatat
    57481 ggtgagatcg tcatcgacat taaccatgtg ccgatgctgg ccaacgcatt cgataaagcc
    57541 cgggcagcgc aaacttcgca gcagcaggaa tggagtacaa tgctgctaag tatgctgcat
    57601 gatattcatc aggaaaacgc catctatttg atggtgagga gactgcgtga ctgacgtttt
    57661 actctgtgtt ggcaatagca tgatgggcga tgatggcgca ggcccgctgc tggcggaaaa
    57721 gtgcgccgcc gcgccgaaag gtaactgggt ggtgattgac ggcggtagtg caccggaaaa
    57781 cgacatcgtc gccatccgtg aactgcgccc tacgcgactg ctgattgtcg acgccactga
    57841 tatggggcta aaccccggcg agatccgcat catcgacccg gatgatatcg ccgagatgtt
    57901 tatgatgact acccataaca tgccgttgaa ctaccttatc gaccagttga aagaagatat
    57961 tggcgaagtg attttcctcg gcatccagcc ggatatcgtc ggcttttact acccgatgac
    58021 ccagccgatt aaagatgcgg ttgaaaccgt ttatcaacga ctggaaggct gggaaggaaa
    58081 tggtggattt gcgcagttag cggtggaagt agagtagttt ttcattaagg aattcaggga
    58141 attgaacatc agaacaggga tgtgcgcttt tttattgagc ttagtgctgc ctgcacaggc
    58201 caccagcttt accgaatatc tgccgatgag tgacagcgaa tacgcgcaaa agcgggcgct
    58261 gaaacctctc ctgaccatgc cttatgatgc cgagcaaaac tggcatttta gaaaagtcgg
    58321 cgttgctggc gtgacgcttg aaaagatgcc aaatgatgat agtgaatggc aattgaacgg
    58381 taaagatcgc gccggtaaat cctggtctgt gcctgtgggg gtgctgcaaa atatggcggg
    58441 taatgcgcaa ctttaccgtg ccgatcttga tcgtaacggc attcaggatc tggttatctg
    58501 gcggggtatt tcaggaaatg gtctggcacc taacgcgttt ttgattctga tgacctttaa
    58561 tcagcaaggt cgcccgtgtg tttttcagtc cgatggtttt tataccgcaa gtgaaactgg
    58621 cattgatgat ttgctggatt tacaaaggaa tggccacacg caattgctgg atatgcagtt
    58681 tgattccggt tactggataa ccagtctgta tcaggtaaaa gacgccaaat ggcagcgtgt
    58741 tcacggctgg tttggcaagt taagctatcc cgcgctcacg cgttttactt atacccccaa
    58801 ccgtaaactc gtcctcaaac ccatcgccgg gcgcgacccg caaacggaag atctggcgca
    58861 gacgcagcgt tgcttgataa agggggatgt gctttatggg gtgaaccaga actgactttt
    58921 tgcagagtgg ctatgagggg gattacgtgc agacagacgt cacgttacta cagagagaac
    58981 aaagaatgac tgctaatgct gcacgcgctg ttaaagcaac aagagaactg gtcaatgcag
    59041 tcccctttct gggaggcagt gactcagaag atgattatcg tgaagcactg gaactggtcg
    59101 aatatttgat tgaagaagat gacaccaatc cacttatcga ctttctggcg agccgcattg
    59161 cagagtatga aaacaacaac gaaaagtttg cagagttcga caaagccgtt gcggcaatgc
    59221 cggttggcgt ggcgttactt cgcacgttga ttgatcaaca taatctgacc tatgcggatt
    59281 tgaagaacga aatcggctct aaatccctgg tcagtcagat tttgtctgga caacgatccc
    59341 tgacaatatc ccacattaag gcactttcag cccgatttgg cgttaagccg gaatggtttc
    59401 tttgattttg actgatgcca aaccccagat tgcccgatgc gctgcgctta t
//
LOCUS       DAZGAN020000016        57908 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGAN020000016 DAZGAN020000000
VERSION     DAZGAN020000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 57908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.016"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            151..1839
                     /locus_tag="ACHKYO_RS05830"
                     /old_locus_tag="pgaptmp_001166"
     CDS             151..1839
                     /locus_tag="ACHKYO_RS05830"
                     /old_locus_tag="pgaptmp_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HGU4428047.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKAR
                     AEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
     gene            1836..2750
                     /locus_tag="ACHKYO_RS05835"
                     /old_locus_tag="pgaptmp_001167"
     CDS             1836..2750
                     /locus_tag="ACHKYO_RS05835"
                     /old_locus_tag="pgaptmp_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU4428048.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVPGGYDW
                     QHVRQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEQALRNGLF"
     gene            2782..3030
                     /locus_tag="ACHKYO_RS05840"
                     /old_locus_tag="pgaptmp_001168"
     CDS             2782..3030
                     /locus_tag="ACHKYO_RS05840"
                     /old_locus_tag="pgaptmp_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4428049.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            3030..4202
                     /locus_tag="ACHKYO_RS05845"
                     /old_locus_tag="pgaptmp_001169"
     CDS             3030..4202
                     /locus_tag="ACHKYO_RS05845"
                     /old_locus_tag="pgaptmp_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGU4428050.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFK
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4238..5317)
                     /gene="lptG"
                     /locus_tag="ACHKYO_RS05850"
                     /old_locus_tag="pgaptmp_001170"
     CDS             complement(4238..5317)
                     /gene="lptG"
                     /locus_tag="ACHKYO_RS05850"
                     /old_locus_tag="pgaptmp_001170"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU4428051.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMNLEELSVPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5314..6384)
                     /gene="lptF"
                     /locus_tag="ACHKYO_RS05855"
                     /old_locus_tag="pgaptmp_001171"
     CDS             complement(5314..6384)
                     /gene="lptF"
                     /locus_tag="ACHKYO_RS05855"
                     /old_locus_tag="pgaptmp_001171"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU4428052.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            complement(6415..6975)
                     /locus_tag="ACHKYO_RS05860"
                     /old_locus_tag="pgaptmp_001172"
     CDS             complement(6415..6975)
                     /locus_tag="ACHKYO_RS05860"
                     /old_locus_tag="pgaptmp_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU4428053.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6987..7811)
                     /locus_tag="ACHKYO_RS05865"
                     /old_locus_tag="pgaptmp_001173"
     CDS             complement(6987..7811)
                     /locus_tag="ACHKYO_RS05865"
                     /old_locus_tag="pgaptmp_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4428054.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSNIRQRLELLISRFPL"
     gene            complement(7811..9160)
                     /locus_tag="ACHKYO_RS05870"
                     /old_locus_tag="pgaptmp_001174"
     CDS             complement(7811..9160)
                     /locus_tag="ACHKYO_RS05870"
                     /old_locus_tag="pgaptmp_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HGU4428055.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9206..9964)
                     /locus_tag="ACHKYO_RS05875"
                     /old_locus_tag="pgaptmp_001175"
     CDS             complement(9206..9964)
                     /locus_tag="ACHKYO_RS05875"
                     /old_locus_tag="pgaptmp_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4428056.1"
                     /translation="MDALQTQTVISTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(9996..10709)
                     /gene="yghS"
                     /locus_tag="ACHKYO_RS05880"
                     /old_locus_tag="pgaptmp_001176"
     CDS             complement(9996..10709)
                     /gene="yghS"
                     /locus_tag="ACHKYO_RS05880"
                     /old_locus_tag="pgaptmp_001176"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4428057.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPA
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            10883..11575
                     /gene="yghT"
                     /locus_tag="ACHKYO_RS05885"
                     /old_locus_tag="pgaptmp_001177"
     CDS             10883..11575
                     /gene="yghT"
                     /locus_tag="ACHKYO_RS05885"
                     /old_locus_tag="pgaptmp_001177"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4428058.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11624..13123)
                     /gene="pitB"
                     /locus_tag="ACHKYO_RS05890"
                     /old_locus_tag="pgaptmp_001178"
     CDS             complement(11624..13123)
                     /gene="pitB"
                     /locus_tag="ACHKYO_RS05890"
                     /old_locus_tag="pgaptmp_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HGU4428059.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(13415..15274)
                     /gene="gss"
                     /locus_tag="ACHKYO_RS05895"
                     /old_locus_tag="pgaptmp_001179"
     CDS             complement(13415..15274)
                     /gene="gss"
                     /locus_tag="ACHKYO_RS05895"
                     /old_locus_tag="pgaptmp_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HGU4428060.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            15479..16345
                     /gene="yghU"
                     /locus_tag="ACHKYO_RS05900"
                     /old_locus_tag="pgaptmp_001180"
     CDS             15479..16345
                     /gene="yghU"
                     /locus_tag="ACHKYO_RS05900"
                     /old_locus_tag="pgaptmp_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGU4428061.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLARGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(16374..16455)
                     /locus_tag="ACHKYO_RS05905"
                     /old_locus_tag="pgaptmp_001181"
                     /pseudo
     CDS             complement(16374..16455)
                     /locus_tag="ACHKYO_RS05905"
                     /old_locus_tag="pgaptmp_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16468..16716)
                     /gene="hybG"
                     /locus_tag="ACHKYO_RS05910"
                     /old_locus_tag="pgaptmp_001182"
     CDS             complement(16468..16716)
                     /gene="hybG"
                     /locus_tag="ACHKYO_RS05910"
                     /old_locus_tag="pgaptmp_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HGU4428062.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(16729..17070)
                     /gene="hypA"
                     /locus_tag="ACHKYO_RS05915"
                     /old_locus_tag="pgaptmp_001183"
     CDS             complement(16729..17070)
                     /gene="hypA"
                     /locus_tag="ACHKYO_RS05915"
                     /old_locus_tag="pgaptmp_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU4428063.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(17063..17551)
                     /gene="hybE"
                     /locus_tag="ACHKYO_RS05920"
                     /old_locus_tag="pgaptmp_001184"
     CDS             complement(17063..17551)
                     /gene="hybE"
                     /locus_tag="ACHKYO_RS05920"
                     /old_locus_tag="pgaptmp_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HGU4428064.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPMYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(17544..18038)
                     /gene="hybD"
                     /locus_tag="ACHKYO_RS05925"
                     /old_locus_tag="pgaptmp_001185"
     CDS             complement(17544..18038)
                     /gene="hybD"
                     /locus_tag="ACHKYO_RS05925"
                     /old_locus_tag="pgaptmp_001185"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HGU4428065.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(18038..19741)
                     /gene="hybC"
                     /locus_tag="ACHKYO_RS05930"
                     /old_locus_tag="pgaptmp_001186"
     CDS             complement(18038..19741)
                     /gene="hybC"
                     /locus_tag="ACHKYO_RS05930"
                     /old_locus_tag="pgaptmp_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HGU4428066.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(19738..20916)
                     /gene="hybB"
                     /locus_tag="ACHKYO_RS05935"
                     /old_locus_tag="pgaptmp_001187"
     CDS             complement(19738..20916)
                     /gene="hybB"
                     /locus_tag="ACHKYO_RS05935"
                     /old_locus_tag="pgaptmp_001187"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HGU4428067.1"
                     /translation="MSHDPKPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(20906..21892)
                     /gene="hybA"
                     /locus_tag="ACHKYO_RS05940"
                     /old_locus_tag="pgaptmp_001188"
     CDS             complement(20906..21892)
                     /gene="hybA"
                     /locus_tag="ACHKYO_RS05940"
                     /old_locus_tag="pgaptmp_001188"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HGU4428068.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(21895..23013)
                     /gene="hybO"
                     /locus_tag="ACHKYO_RS05945"
                     /old_locus_tag="pgaptmp_001189"
     CDS             complement(21895..23013)
                     /gene="hybO"
                     /locus_tag="ACHKYO_RS05945"
                     /old_locus_tag="pgaptmp_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HGU4428069.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(23202..23489)
                     /gene="yghW"
                     /locus_tag="ACHKYO_RS05950"
                     /old_locus_tag="pgaptmp_001190"
     CDS             complement(23202..23489)
                     /gene="yghW"
                     /locus_tag="ACHKYO_RS05950"
                     /old_locus_tag="pgaptmp_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HGU4428070.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(23608..24495)
                     /gene="yghX"
                     /locus_tag="ACHKYO_RS05955"
                     /old_locus_tag="pgaptmp_001191"
     CDS             complement(23608..24495)
                     /gene="yghX"
                     /locus_tag="ACHKYO_RS05955"
                     /old_locus_tag="pgaptmp_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HGU4428071.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPARMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            24652..25692
                     /gene="gpr"
                     /locus_tag="ACHKYO_RS05960"
                     /old_locus_tag="pgaptmp_001192"
     CDS             24652..25692
                     /gene="gpr"
                     /locus_tag="ACHKYO_RS05960"
                     /old_locus_tag="pgaptmp_001192"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HGU4428072.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(25732..26226)
                     /gene="yqhA"
                     /locus_tag="ACHKYO_RS05965"
                     /old_locus_tag="pgaptmp_001193"
     CDS             complement(25732..26226)
                     /gene="yqhA"
                     /locus_tag="ACHKYO_RS05965"
                     /old_locus_tag="pgaptmp_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HGU4428073.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            26417..27301
                     /gene="yghA"
                     /locus_tag="ACHKYO_RS05970"
                     /old_locus_tag="pgaptmp_001194"
     CDS             26417..27301
                     /gene="yghA"
                     /locus_tag="ACHKYO_RS05970"
                     /old_locus_tag="pgaptmp_001194"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU4428074.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(27340..27765)
                     /gene="exbD"
                     /locus_tag="ACHKYO_RS05975"
                     /old_locus_tag="pgaptmp_001195"
     CDS             complement(27340..27765)
                     /gene="exbD"
                     /locus_tag="ACHKYO_RS05975"
                     /old_locus_tag="pgaptmp_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HGU4428075.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(27772..28506)
                     /gene="exbB"
                     /locus_tag="ACHKYO_RS05980"
                     /old_locus_tag="pgaptmp_001196"
     CDS             complement(27772..28506)
                     /gene="exbB"
                     /locus_tag="ACHKYO_RS05980"
                     /old_locus_tag="pgaptmp_001196"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU4428076.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            28758..29945
                     /gene="metC"
                     /locus_tag="ACHKYO_RS05985"
                     /old_locus_tag="pgaptmp_001197"
     CDS             28758..29945
                     /gene="metC"
                     /locus_tag="ACHKYO_RS05985"
                     /old_locus_tag="pgaptmp_001197"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGU4428077.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            30085..30744
                     /gene="yghB"
                     /locus_tag="ACHKYO_RS05990"
                     /old_locus_tag="pgaptmp_001198"
     CDS             30085..30744
                     /gene="yghB"
                     /locus_tag="ACHKYO_RS05990"
                     /old_locus_tag="pgaptmp_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HGU4428078.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(30784..31683)
                     /gene="yqhC"
                     /locus_tag="ACHKYO_RS05995"
                     /old_locus_tag="pgaptmp_001199"
     CDS             complement(30784..31683)
                     /gene="yqhC"
                     /locus_tag="ACHKYO_RS05995"
                     /old_locus_tag="pgaptmp_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HGU4428079.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            31877..33040
                     /gene="yqhD"
                     /locus_tag="ACHKYO_RS06000"
                     /old_locus_tag="pgaptmp_001200"
     CDS             31877..33040
                     /gene="yqhD"
                     /locus_tag="ACHKYO_RS06000"
                     /old_locus_tag="pgaptmp_001200"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HGU4428080.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            33145..33972
                     /gene="dkgA"
                     /locus_tag="ACHKYO_RS06005"
                     /old_locus_tag="pgaptmp_001201"
     CDS             33145..33972
                     /gene="dkgA"
                     /locus_tag="ACHKYO_RS06005"
                     /old_locus_tag="pgaptmp_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HGU4428081.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            34172..35098
                     /gene="yqhG"
                     /locus_tag="ACHKYO_RS06010"
                     /old_locus_tag="pgaptmp_001202"
     CDS             34172..35098
                     /gene="yqhG"
                     /locus_tag="ACHKYO_RS06010"
                     /old_locus_tag="pgaptmp_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HGU4428082.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            35149..35406
                     /gene="yqhH"
                     /locus_tag="ACHKYO_RS06015"
                     /old_locus_tag="pgaptmp_001203"
     CDS             35149..35406
                     /gene="yqhH"
                     /locus_tag="ACHKYO_RS06015"
                     /old_locus_tag="pgaptmp_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HGU4428083.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(35449..37668)
                     /gene="ygiQ"
                     /locus_tag="ACHKYO_RS06020"
                     /old_locus_tag="pgaptmp_001204"
     CDS             complement(35449..37668)
                     /gene="ygiQ"
                     /locus_tag="ACHKYO_RS06020"
                     /old_locus_tag="pgaptmp_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HGU4428084.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(37779..39191)
                     /gene="ftsP"
                     /locus_tag="ACHKYO_RS06025"
                     /old_locus_tag="pgaptmp_001205"
     CDS             complement(37779..39191)
                     /gene="ftsP"
                     /locus_tag="ACHKYO_RS06025"
                     /old_locus_tag="pgaptmp_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HGU4428085.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(39266..40003)
                     /gene="plsC"
                     /locus_tag="ACHKYO_RS06030"
                     /old_locus_tag="pgaptmp_001206"
                     /gene_synonym="parF"
     CDS             complement(39266..40003)
                     /gene="plsC"
                     /locus_tag="ACHKYO_RS06030"
                     /old_locus_tag="pgaptmp_001206"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HGU4428086.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(40236..42494)
                     /gene="parC"
                     /locus_tag="ACHKYO_RS06035"
                     /old_locus_tag="pgaptmp_001207"
     CDS             complement(40236..42494)
                     /gene="parC"
                     /locus_tag="ACHKYO_RS06035"
                     /old_locus_tag="pgaptmp_001207"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGU4428087.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(43040..43522)
                     /gene="ygiV"
                     /locus_tag="ACHKYO_RS06040"
                     /old_locus_tag="pgaptmp_001208"
     CDS             complement(43040..43522)
                     /gene="ygiV"
                     /locus_tag="ACHKYO_RS06040"
                     /old_locus_tag="pgaptmp_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGU4428088.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(43575..43967)
                     /gene="ygiW"
                     /locus_tag="ACHKYO_RS06045"
                     /old_locus_tag="pgaptmp_001209"
     CDS             complement(43575..43967)
                     /gene="ygiW"
                     /locus_tag="ACHKYO_RS06045"
                     /old_locus_tag="pgaptmp_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HGU4428089.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            44119..44778
                     /gene="qseB"
                     /locus_tag="ACHKYO_RS06050"
                     /old_locus_tag="pgaptmp_001210"
     CDS             44119..44778
                     /gene="qseB"
                     /locus_tag="ACHKYO_RS06050"
                     /old_locus_tag="pgaptmp_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HGU4428090.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            44775..46124
                     /gene="qseC"
                     /locus_tag="ACHKYO_RS06055"
                     /old_locus_tag="pgaptmp_001211"
     CDS             44775..46124
                     /gene="qseC"
                     /locus_tag="ACHKYO_RS06055"
                     /old_locus_tag="pgaptmp_001211"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HGU4428091.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(46170..46502)
                     /gene="ygiZ"
                     /locus_tag="ACHKYO_RS06060"
                     /old_locus_tag="pgaptmp_001212"
     CDS             complement(46170..46502)
                     /gene="ygiZ"
                     /locus_tag="ACHKYO_RS06060"
                     /old_locus_tag="pgaptmp_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HGU4428092.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
     gene            46821..47402
                     /gene="mdaB"
                     /locus_tag="ACHKYO_RS06065"
                     /old_locus_tag="pgaptmp_001213"
     CDS             46821..47402
                     /gene="mdaB"
                     /locus_tag="ACHKYO_RS06065"
                     /old_locus_tag="pgaptmp_001213"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HGU4428093.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            47433..47747
                     /gene="ygiN"
                     /locus_tag="ACHKYO_RS06070"
                     /old_locus_tag="pgaptmp_001214"
     CDS             47433..47747
                     /gene="ygiN"
                     /locus_tag="ACHKYO_RS06070"
                     /old_locus_tag="pgaptmp_001214"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HGU4428094.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(47795..49687)
                     /gene="parE"
                     /locus_tag="ACHKYO_RS06075"
                     /old_locus_tag="pgaptmp_001215"
     CDS             complement(47795..49687)
                     /gene="parE"
                     /locus_tag="ACHKYO_RS06075"
                     /old_locus_tag="pgaptmp_001215"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGU4428095.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQHRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(49716..50297)
                     /gene="yqiA"
                     /locus_tag="ACHKYO_RS06080"
                     /old_locus_tag="pgaptmp_001216"
     CDS             complement(49716..50297)
                     /gene="yqiA"
                     /locus_tag="ACHKYO_RS06080"
                     /old_locus_tag="pgaptmp_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HGU4428096.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(50297..51124)
                     /gene="cpdA"
                     /locus_tag="ACHKYO_RS06085"
                     /old_locus_tag="pgaptmp_001217"
     CDS             complement(50297..51124)
                     /gene="cpdA"
                     /locus_tag="ACHKYO_RS06085"
                     /old_locus_tag="pgaptmp_001217"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HGU4428097.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPYQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(51149..51571)
                     /gene="yqiB"
                     /locus_tag="ACHKYO_RS06090"
                     /old_locus_tag="pgaptmp_001218"
     CDS             complement(51149..51571)
                     /gene="yqiB"
                     /locus_tag="ACHKYO_RS06090"
                     /old_locus_tag="pgaptmp_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HGU4428098.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(51572..52201)
                     /gene="nudF"
                     /locus_tag="ACHKYO_RS06095"
                     /old_locus_tag="pgaptmp_001219"
     CDS             complement(51572..52201)
                     /gene="nudF"
                     /locus_tag="ACHKYO_RS06095"
                     /old_locus_tag="pgaptmp_001219"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HGU4428099.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            52406..53887
                     /gene="tolC"
                     /locus_tag="ACHKYO_RS06100"
                     /old_locus_tag="pgaptmp_001220"
     CDS             52406..53887
                     /gene="tolC"
                     /locus_tag="ACHKYO_RS06100"
                     /old_locus_tag="pgaptmp_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HGU4428100.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            54035..54706
                     /gene="ygiB"
                     /locus_tag="ACHKYO_RS06105"
                     /old_locus_tag="pgaptmp_001221"
     CDS             54035..54706
                     /gene="ygiB"
                     /locus_tag="ACHKYO_RS06105"
                     /old_locus_tag="pgaptmp_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HGU4428101.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            54712..55872
                     /gene="ygiC"
                     /locus_tag="ACHKYO_RS06110"
                     /old_locus_tag="pgaptmp_001222"
     CDS             54712..55872
                     /gene="ygiC"
                     /locus_tag="ACHKYO_RS06110"
                     /old_locus_tag="pgaptmp_001222"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU4428102.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(55910..56698)
                     /gene="ygiD"
                     /locus_tag="ACHKYO_RS06115"
                     /old_locus_tag="pgaptmp_001223"
                     /pseudo
     CDS             complement(55910..56698)
                     /gene="ygiD"
                     /locus_tag="ACHKYO_RS06115"
                     /old_locus_tag="pgaptmp_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
     gene            56841..57614
                     /gene="zupT"
                     /locus_tag="ACHKYO_RS06120"
                     /old_locus_tag="pgaptmp_001224"
     CDS             56841..57614
                     /gene="zupT"
                     /locus_tag="ACHKYO_RS06120"
                     /old_locus_tag="pgaptmp_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HGU4428103.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAAMGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(57672..57842)
                     /gene="yqiD"
                     /locus_tag="ACHKYO_RS06125"
                     /old_locus_tag="pgaptmp_001225"
     CDS             complement(57672..57842)
                     /gene="yqiD"
                     /locus_tag="ACHKYO_RS06125"
                     /old_locus_tag="pgaptmp_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HGU4428104.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT        14372 a        15384 c        14474 g        13678 t
ORIGIN      
        1 taaatcaatg ttcattttca caccgatgaa aatactatct catttatctt ttatgcctat
       61 ataatccacc gcattattgt cattttattt tggatatcca attttttcgt ggtgtatatg
      121 tctaataaaa tctttacgca ttccctacct atgcgctatg ccgattttcc aacgctggtt
      181 gatgctttgg actacgccgc tctgagtagc gccggaatga atttttatga cagacgttgc
      241 caacttgaag atcaactgga atatcagacg ttaaaagcac gtgccgaagc tggtgcgaag
      301 cggttgttat cgctgaacct gaaaaaaggc gatcgcgtgg cactgattgc cgaaacaagt
      361 agcgggttcg tagaggcttt ttttgcctgc cagtatgccg gcttagtcgc cgtcccgttg
      421 gcgattccaa tgggcgttgg tcagcgggat tcctggagcg ccaaattgca gggtttactg
      481 gcaagttgcc agcccgcagc cattatcact ggtgatgagt ggttgccact ggtcaatgcc
      541 gcgacgcata acaaccccga attacatgtt ttaagccacg cctggtttaa ggcattaccg
      601 gaagccgatg ttgcgctcca gcgtccagtt ccgaacgata tcgcctacct ccagtacacc
      661 tccggcagca cccgttttcc ccgtggcgtc attatcaccc atcgcgaagt gatggctaat
      721 ctacgtgcta taagccacga cggcattaaa ttacgccctg gcgaccgctg cgtctcctgg
      781 ctgcctttct accatgatat gggactggtc ggctttctcc tgacccccgt cgccacgcag
      841 ctttcagtag attatttgcg cactcaggat tttgccatgc gtcctctgca atggcttaaa
      901 ttgatcagta aaaatcgcgg caccgtttcc gttgcgccgc cgtttggcta tgaattgtgc
      961 cagcgccgcg tgaatgaaaa agatctcgct gaactggatc tttcctgctg gcgcgtcgct
     1021 ggtattggtg cagaacccat ctccgcagaa caactccatc aattcgctga atgtttccgt
     1081 caggttaact ttgacaataa aactttcatg ccgtgctacg gactggcaga aaatgcgctg
     1141 gctgtcagct tctctgatga agcctccggg gttgtggtta acgaagtgga tcgcgacatc
     1201 ctcgaatatc agggtaaagc cgtcgcgccg ggtgcagaga cacgcgccgt atcgactttc
     1261 gtcaactgcg gcaaagcgtt gccggaacat ggtattgaaa tccgcaatga agcaggtatg
     1321 ccggtcgcgg aacgtgtggt aggccatatt tgcatctccg gccccagtct gatgagcggt
     1381 tactttggcg accaggcttc gcaagacgag attgccgcga cgggctggtt agacaccggc
     1441 gacctcggtt atctgctgga cggttatctg tatgtcaccg gacgcattaa agatctgatt
     1501 attattcgtg gccgtaatat ctggccgcag gatattgaat atatagcgga acaggaaccg
     1561 gaaattcatt ctggcgatgc gattgctttt gttaccgccc aggaaaaaat cattttgcag
     1621 atccagtgtc ggatcagcga tgaagaacgt cgcggacagc ttatccacgc gctggcagca
     1681 cggatccaaa gcgaatttgg cgtaaccgcg gctatcgatc tgttgccgcc ccacagcatt
     1741 ccccgaacgt cctccggcaa gcctgcccgt gcggaagcga aaaaacgtta tcagaaggct
     1801 tatgctgcaa gccttcatgt gcaggaatcc ctggcatgaa tcaaactgtc gcggtgacgg
     1861 gcgctaccgg gtttatcggt aaatatatta tcgataacct gctcgcccgc ggctttcacg
     1921 ttcgcgcatt gacgcgtact gcccgcgctc acgtcaatga taatcttact tgggtgcgcg
     1981 gttcgctgga agatacacat tcgcttagcg aactggttgc cggagccagc gcggtggtcc
     2041 attgcgccgg gcaagtgcgc ggacacaaag aagagatttt tacccactgt aacgttgacg
     2101 gcagcctgcg cctgatgcaa gcagcaaaag agagcggctt ttgccaacgt tttctgttta
     2161 tctcttcgct ggcggcgcgc catcccgagc tctcctggta cgcaaattcc aaacacgtcg
     2221 ccgaacaacg tctgactgca atggctgacg aaattacgct gggcgttttt cgcccgacag
     2281 ccgtgtatgg tcccggcgat aaagagttaa aaccgctgtt tgactggatg ctgcgcggcc
     2341 tgctgccacg acttggtgcg ccagatacac agctctcttt cctgcacgtc accgatttcg
     2401 cgcaagcagt gggtcagtgg ttaagcgccg aaactgtaca gacgcaaacc tatgaattat
     2461 gcgatggcgt ccccggcggc tatgactggc aacacgtacg acagcttgcc gccgacgccc
     2521 gttgtggttc cgtgcgaatg gttggtattc ctctgccggt actcacctgc cttgcggata
     2581 tcagtaccgc gttgagtcgc ctggcaggta aagaacctat gctgacccgc tcgaaaattc
     2641 gtgaattaac ccacgccgac tggtcggcaa gtaataaccg tatttctgaa gatattaatt
     2701 ggtttcccgg gattagcctg gaacaagcat tacgcaacgg gctattttga ttgtcttgtt
     2761 taaaggtgtg ttaacgtttc tatggtaaat cgtgaaatag taatggatta catcctttcc
     2821 tgtttacagg atttggtcga aaacggtgtg gaaattaagc ccgacagcga tctcgtcaac
     2881 gatcttggcc tggagtcgat caaagtgatg gatctcttaa tgatgctgga agacagattc
     2941 gatatttcca ttcctattaa tattctgctg gatgtcaaaa cccctgcgca attaatggaa
     3001 actctacttc catggctgga gaataaataa tggggctata cgataaatat gcgcgccttg
     3061 ctggcgagcg actgcaattt agtgacaacg ggttaacacc gtttggcacc tgtattgatg
     3121 aagtgtattc cgccaccgaa ggccggatcg gcaataaaaa agtgattctg gccgggacca
     3181 ataattacct cggtttaacc tttaatcatg atgccatcgc cgaaggtcag gcagcgttag
     3241 cggcccaagg caccggcacc accggttctc gtatggcaaa cggcagttat gcgccgcacc
     3301 tggcgttaga gaaagagatt gccgagtttt tcaagcgccc taccgctatc gttttttcaa
     3361 ccggttatac cgctaattta ggcgtcatca gcgcacttgc cgaccataac gccgtggtgt
     3421 tactggatgc cgacagccac gccagtattt atgacgcctg ttctctcggt ggcgcggaaa
     3481 ttattcgttt ccgccataac gatgctaaag atctggaacg gcgcatggtt cgcctcggtg
     3541 aacgggcgaa agaggcgatt atcatcgttg agggcatcta cagcatgctc ggtgacgttg
     3601 cgccgctggc ggaaattgtt gatatcaaac gtcgtctcgg tggttatttg attgtcgatg
     3661 aagcgcattc gtttggcgtg ttaggcgcaa cggggcgcgg actggctgaa gctgtcggcg
     3721 ttgaagatga tgtggatatt atcgttggca ccttcagtaa aagtctggcc tcgattggcg
     3781 gttttgctgt cggttcggaa gcgatggaag tgctgcgcta cggcagccgc ccgtatattt
     3841 ttaccgcctc tccttctccg tcctgcattg cgaccgtgcg ctcgtcgtta agaactattg
     3901 ccacacaacc agagctgcgg caaaagctga tggataacgc caaccatctg tatgacggtc
     3961 tgcaaaaact gggttatgag ttaagttcac atattagccc ggtagtgcca gtaataatcg
     4021 gttcgaaaga ggaaggttta cgcatctggc gcgagcttat ctcgctgggc gtgtatgtga
     4081 atcttatcct gccgcctgcc gcgcctgcag gcatcacgct gctgcgctgt agcgtaaatg
     4141 ctgcccacag tcgtgagcaa atcgatgcca tcattcaggc atttgctact ctgaagcagt
     4201 aatgtgagcc cgccgtggtg tttgcgccac ggcgggttta atgttgtctg tcataccaat
     4261 acaccaggct caacgccacc aacacaaacg ctaccggtaa accgagcgcg gtaacaggtg
     4321 cgcttaacgc aaatagcaat cccagattaa ccatgatttg atagctgatc caggtgagta
     4381 agcccacgat gacgcctaca gcgagacggc tacccatccc aggcgaacgc ggggcagtaa
     4441 aggtaaacgg cacagccagc aaaatcatcg ccagggtcag gatgggttgc cccagttttt
     4501 cccacagtgc aaggcggtat tcgctgctgg gttgcccggt ttcctgcaaa taatggatgt
     4561 aatgattaag ctgacggaca gaaaaagtat tccccggcac tgataactct tcaagattca
     4621 tactggtgaa ggccgattgc caggcaagat tatctgatgt ttccagcgtt tctttaccgt
     4681 taagccattt tttatgattc acaccatgca ggatccacgt tttgtcgtct ttaatggtgg
     4741 cactgcgtgc ataaatgtag gattcgagcg aaagatcgtc acgataatga aaaatctcca
     4801 cgcccacagg ctggccctgt tcattcagcg atttcaccgt cacaaattca ttgccgcgcc
     4861 tggcccacag catattgccg gtaatgtcat cgtcctcccc caacgcggtg gcggtcgatt
     4921 tgatttgcag cgcctgttgc tgcaatggcg acgccaccca ctcatctatc gcgcctaacg
     4981 aaacagtcca caatatcccg gcaaccagcg ccaccagtgc aatacggaag atagaaaacc
     5041 ccgtgctgcg aatggcggta agctcactgt ttttcgataa ctgccccagg ccaacaatcc
     5101 ctcccaataa ggcgataaac ggcgaaagtt cgaccagtgt gcgcggtaag gtcattagca
     5161 ccaccagcac cgcctgagtc cagcgataac cgcccgggct gacatcatcc agttcgttaa
     5221 tcaggttgaa ggtggtaaaa agcggcagca atagccctgc ggcggcggca aaaccgagaa
     5281 agagatgacg gattaaatag cgactgaaaa cattcatcgg gaaaatttct gcagcaaaga
     5341 gaagtcgcgt gcgaccagca tcagcaggcc cattagcatg agtccgggca ctaaccacaa
     5401 accaggaatg agaggaatcg cgccgttagc caccagcgta cggcagacgt cgccgccata
     5461 aaaaatggca ataaacagca acgtcagtgg caataacgtt gaaaatcgcc cttgccgcgg
     5521 ttttacccga cttaatgaaa tggccagcag cgccatcaac aatgcggtca ggccacggct
     5581 ttgtcgccat tgcagttcgg catgatcggc aggaaaggcg gagcgcgcca gctccgtgac
     5641 cgattttgct ttgcgtttga cgttagggct ttgatccagc ggattcagat gtaattgcag
     5701 attacggtag atctgttcgt tgtcgtcacg cccctgatga tcgagaagat aggcagtgcc
     5761 gttgtgcaac ataacggtcg gtttctcggg tgatgggtca accacatcaa ccgaacgggc
     5821 gcggaaaata cgggttcgat tggcagtaga agtataaatc agcgcgtcag tcaggcgatt
     5881 attatcctga tcaaccgtct gcgaaaggat cattcgtccg ttatcgttag tgttaaattt
     5941 ctttgcccgc aactggcgaa catccagctc cgactgcgac tgctgctcca gttgataaat
     6001 ctgcgcataa gcccacggac gaccatacat cgacagaagg gtgacaaaaa tacttagcgg
     6061 tatcgccaga taaagaacgg ctttgtacaa gcgtcccgga ctgccccccg ccgcagagat
     6121 agcggtaatt tccgagtcgg tatacatctg ccctagcgtc acgccaactg acacatacag
     6181 accaacaggt aacaacatct ccagtgcaat cagtactttg tagaaaacga tatccagcac
     6241 aacatcaaga gctaatgtcc catttgccgc ttcggtcaga taacgctgtg cggagtagct
     6301 ggcgaaaata aagatcagaa aaccgacgat aatcagcacc agacggcgcg taccacgcat
     6361 gatgtagtgt tcaaccagct tcatctcact aacgccctca atcctttgag ttttttattc
     6421 attgatcagt ttctgcgcca acgcaatgac atgatctacc tcttccggcg tcaggctgcc
     6481 gtcctttgcc cactgaattt gcccggcttt attcagcacc agaatggttg aactctgctc
     6541 cggcaagcgc caggcaacgc gtccctgtcc attgccgtca ataacgaatt gcgcccacgg
     6601 atagcggcgt ttattttttt cgattttgcc aacaacaaaa tagccagtac caaagatggc
     6661 atcatcggtg ttcacaatgg tggtgggctg aaaacgatcc tgcggaaagt ttgccgcttc
     6721 aaccgcttta attaacagtg agttcttttt cttcgccgac ttacgcccgg cgatatactg
     6781 gataatgcgg acttttcccg ccaattgtgt gctgttccac ttttggtagt gaatttcgtc
     6841 gtcgctattc agaagcagct cgccgccttc ctgaatataa acaggggtaa ccgttttgcc
     6901 agtgacgaaa ttgtgtgcct gcacacccgg ggctaaaagc atgataatca gcgcaataat
     6961 caaacgagat gacatcattg ctccggttat agcggaaaac gcgaaatcag cagctctaag
     7021 cgctgacgaa tattgctatc cagcgccaac ggtgcatcat acgactggcg gttttccata
     7081 tcatccgcca ccatgtcgtc gtgtttatct cccatcaggc cattaatggc ggcgaggaaa
     7141 cgggaaatct gtaacgcctc tttattttca ggatcaagcg atcttaaggt gtcgtaataa
     7201 gaaacctgtt ggctgatgat ttttcgcaca tcatgaaatt ccctgccggt tagtcgcggt
     7261 ttggcaataa tctctttcag ggtatgcatt tgcttttgcc gataggcaat aaaactgctt
     7321 acgctttcta actggctatg gcgtaatgaa tagttcacca acgaccagac cggagagctt
     7381 aaataaatcc gtaacaggtt gccgtaatac gacacaatat ttttcttacc gttacgaaag
     7441 ccgtcctgaa aacgcccaag aaaaacggtc gttttgccga ataaaaatcc ggcctgatgc
     7501 ttctttaaat acagccgttg cgcaaaacgc aggtgcttgt agcgcgctcg catgtattta
     7561 aattgtttgc gctcatcagc agtcagctca tcgcctttgg cttgtttgcg aactaaacgc
     7621 agtaattcct gacgatcaac gccattgagc cagaattgca ggcataacgc gtaattatca
     7681 tgaatgattt cggtggtaca acttagtgca atagtcggcg gcaaactaac ctgtgcatca
     7741 acgacatcat cttcttcaat cgcaacaaat agacgctcgc tcgcctcaag cgggaaatat
     7801 tttctcatca tcaggatttc tctccggctg cgttataaag caaggaatgg cgctgaaaaa
     7861 atgcctttaa ggttggaatt aacgagagcg ccacgtccag acattgccca ccgaagtaag
     7921 caaacgctgc tcccagcaca ccaaacagat gacagaacat gaacagcaat acgatgtagc
     7981 cgattgaggc gatggtttgc gccacgagaa aagcgcgctg ttttcccgcc atcaatagca
     8041 atgactcctg agggaagccc agcatcgaaa tcacaattgc gcccagcatc acctgaatca
     8101 ggtcatacgc ttcgagatac ttaacgccaa acaccagaga aatgagcggc ttgccgacga
     8161 taaacaccgc gagcgccacc agaataccga ttccgcccgc cagtaaaccg gacttcacac
     8221 ctaacaacca cggtctggtg gtgcgcggat ctaaacgcat cacctctggg taaaaacttt
     8281 tacccagcaa acccgctggc gttccggcgg cgtcgaagaa tgtcatggcg attttaaata
     8341 acccggcggc agcgggtcct aacacgatcc ccaccagcac tgtgctgcac gagttacgcg
     8401 ccgaccagat ggagtgggca atgttggttg accagacaaa actccacgcg cctttgatgt
     8461 gtcgggcaga ctcaaacaga ttcaatttga aggcattatg gatatttcgg cggcgtaatt
     8521 cgcgcgcggc aaaccaccag tacatggtgc cgccaaccag attcgacacg taccaggcaa
     8581 taacaaaacc cgcaaaacca aagtcaaaat accaggctac gacgctcccc gctgcgcgca
     8641 gaaaaggttt cgtcgcctgc tgtacagcaa ttaaatcgaa gcgatctacc gcacgcagaa
     8701 tgccggtcgg cgtggaggaa gccattgaag gaatgagcgt gcaatagagc gctgccagcc
     8761 aaaaactttg gtcatccaga cctaatgaat gggaaaggaa tggcagtaag gcaatgccac
     8821 cgacaatcgc caccgcgccg ctgacgatat ccagcgagaa ggaaaatgag acgacattgc
     8881 ggaattgctg cggattattg ttggttaatg ctggtgttcc gtactgaacc accagttgcc
     8941 atgtctgaaa cttaataaaa tcgctgatcg acttggcgta cgattgcaca atcaccagta
     9001 cgccaaacat ggcgggcgtc atccctttac cggcacacga gagcgccaac agccccagca
     9061 aggcgctcac gacattactg gagcctaacc aggcgctatt gcgaataatg gtgcgaaacg
     9121 cgccatctgc aaaccaatgt ttgatgttaa aacccgccaa ttcagcctga ccttatcgtt
     9181 gatagtaaaa agtatcccgc cagccttaag ttaaacttcg gcggtcagaa acgatggcaa
     9241 ccagagaaac cgccttctgt gcctgttcca gcacttcgct gtagggcgct ctggaatcaa
     9301 tctcaagaat ttttgtgccg ttatagccaa tcttcgacat gacaccgatt ttgtcctgca
     9361 gctcggcata gtcatggtca ggcttgcggg agatggcagt ctcaatatca atgcccaggc
     9421 gaataattaa ttccgggcga tattgcgcca tttgttggta taaacgccgt tcgcgctgcg
     9481 ccagaaacat gctgattttc ccggtcgcac gttcgacgcc aatccccggt ccgtcataat
     9541 aaaagcccga aatttcagcc tgcgggaagc gatcgctgac caccagaacg ccactttgcg
     9601 ccagtcgctg aaccttgcgt agattcgcca ttcttcgcag cgagaagcag tacataatca
     9661 ccgccgccca tagcgccgga gatttggttt tcatgctttg ggttttcgag gatttggccg
     9721 ccagtcgccg ttccagccag acgccaacca acggcaatcg tttgattttg tcgccgtctt
     9781 cgccggagag cagccccaga tagcgccgct cggtttgcca gtgttgttgc agcgatttca
     9841 ccaggtcggt ggtcagtgtg gatttaccgg tgccatcaca cccgaccacc gcaattagcc
     9901 ccggaatgta gctgggctgc ggcgcggttg tactaataac agtttgtgtt tgtagtgcat
     9961 ccattcgtta aatcatcccc tatccatttt ggtgatcagg aaagcgcggc gtgaattgcg
    10021 cgtagtgacg cttgcagaat ttcatcggct ggttgccgac catccagttc aaggatcctg
    10081 gcgccgttga aagtcagttg cggcgtaacg gcgatttttt cctgtaacgc tgccagttgg
    10141 tggtcaggtt tacgcgcaaa cgccgtttgt tcatcaatgc caagacgaat caacaatacg
    10201 ggcaaataag atgccatcca ttggtacagc ttcagctcgc gctgccttaa catttttatc
    10261 caaccgttac cgcccgtggt ttttgccaat tgcgggccat caaagcgaaa acccggcact
    10321 tcaacctgcg ggtagcggtc ggtgatgagc agatagcctt gctggctttt acacaacatt
    10381 ttgcgaaact tgtacgcccg ccagcaggaa agcagataga taaccagagc agtaatattg
    10441 ccaggcggtg ttgagggctt ttcgtgcaca tgtgccgctt tacttcgcaa ataacgccca
    10501 aaaggtgcgc caataacagg gagctgtgaa atccattcgc caattcgccc ggacgattgc
    10561 ccgagataaa tgtgttctgc tggcattctt gctgccagtt catttaccag gcttgccgtg
    10621 agggtcgatt tacctgaacc atcacatcct acaatggcaa taacacgcac tggcgtggaa
    10681 ttaattattg acatattacg ttgattcacg aaaaacccgg cagtaaataa tgtattgaat
    10741 attacgtggt ccgtgctcag aatatccgtt caaccttatt gtgacaatgg ttaattgtga
    10801 cattgacctg aattacgctt tcataaaaac atattaacca aataaatatt ttggatggat
    10861 atttaaattt aaggatatat tcatgcagtc aataacacct ccattaattg ccgttattgg
    10921 tagcgatggt tcaggcaagt caacggtgtg tgaacatctc attaccgttg tcgaaaaata
    10981 tggtgctgcc gaaagagttc atttaggaaa acaggccgga aatgtcggtc gtgcagtgac
    11041 aaaattaccg ttgatgggaa aatccttaca taaaacaatt gaacgaaatc aggtgaaaac
    11101 agcaaaaaaa ttgcctggac cagttccggc gctggtaatt acagcgtttg tcgcccgtcg
    11161 cttactgcgc tttcgtcata tgcttgcctg tcgtcgtcgc gggttaattg ttctaaccga
    11221 ccgttatcct caggaccaaa ttcctggcgc ttacgatggt acggtgttcc cacctaacgt
    11281 tgaaggtggt cgttttgtct catggctggc aagccaggaa cgtaaagcgt ttcactggat
    11341 ggcgagccat aagcctgatc tggtcatcaa actcaatgtt gaccttgaag ttgcctgtgc
    11401 acgtaaaccc gaccataaac gggaatcgct ggcgaggaag attgccataa cgccacagtt
    11461 aacctttggt ggtgcacaac tggttgatat cgatgccaat cagccactgg aacaggtgtt
    11521 ggttgatgca gaaaaagcga ttacggattt tatgaccgcg cgtggttatc actagtcaaa
    11581 aatggaaatg cccgatcgcc aggaccgggc attttcagga aggttaaatc aactgcaatg
    11641 ctatccagta cagcccacca gaaagaaaaa ttgccgccgg taaagtaaat acccacgcca
    11701 tcaggatgct ggttaccgtt ttacgctgta acccaccgcc gtccaccacc atcgtccctg
    11761 caactgcaga cgagaggacg tgtgttgtgg agacgggcat cccaatataa ctggcaagac
    11821 cgatagacac tgccgccgtc atttgtgccg ccatgccttg cgcatacgtc atgccgcgct
    11881 taccaatctt ctcaccgatg gtcatcgcta cacgacgcca gccaatcatg gtgccaatgc
    11941 cgagcgccag tgctaccgcc atgatgatcc acaccggagc gtactcaatg gtgcttaaca
    12001 tatcgctgcg cagttttttc agcaggttct ggtcttcttt actgacgcct ggcagtttcg
    12061 ccagcttcgc ggaggtatca gagatgcaca gcataatgcg gcgcagctgg ctgcgctgac
    12121 tcacgcttaa cggctcgtaa ctttccatat tgcctggcag catcgtttta acgcgcgcaa
    12181 tcgcatcaaa ggtatttgcc ggatgacagt gaaactctgt tacttgcgtg ccatcagtcg
    12241 atgctgcagg caatggaggt tccatcgcaa tcaacttctg cggcagttca ggatgctgtt
    12301 gcaggtagtg ttcgaagttg gtgacggcat cgcgggtacg ggtaatttca tagccggacg
    12361 cgttcatatt gacgacgaag ccagcagggg caatccccac cagtaccagc atcaccaggc
    12421 cgatcccttt ttgtccgtcg ttcgcgccgt gcgaaaacgc cacgcccgca gcggaaacaa
    12481 tcagcgcaat acgcgtccag aatggcggtt tacgtttgcc ttttttcttt ttgcgatctt
    12541 ccggaatgcg gtgaatacgg tcacgctttt tcgtcccgct ccagtagcgt cgcagcagga
    12601 atatcaggcc tcccgcaatg accaggccga cgatagggga aacaatcagc gaggagaaaa
    12661 ttttggtcac ttcacgcagg tttaacgcat ccatcaccga tgagccggtt aacagcgcgt
    12721 tggttaaacc gatgccgata atcgcaccaa tcaaggtgtg cgaactggag gccggtaaac
    12781 cgaagaacca cgttcccagg ttccagataa tcgccgccag cagcatggaa aagaccatcg
    12841 ccaggccgtg ggttgacccc atattcagca acaaatcggt tggcaacata tggacaatgg
    12901 cataggcaac gctaagtccg cccaataaca cgccaaaaaa gttaaaaaat gccgccatca
    12961 ccacagcaag ttgtggttgc atggcacgag tataaataac ggttgccacc gcattcgccg
    13021 tgtcatgaaa accattgatt gcttcgtaga acaacacaaa tgccagagca agcaataaca
    13081 aaagccctgt gtatatatca aggccaacaa ataaatttag catatattag attacgccat
    13141 tttgaattta cgaacggacg cattatcagt gactttaacg gcatgggcaa agtgaaatat
    13201 cattttttaa cgataaaaag agtgttattt cttgtgtatc agacaataag cattcattca
    13261 aaacctttag aaataatcaa aacacaggta ttccactggt taatatattc acatatgaaa
    13321 tgaataaata ttagaacata taaatattga atattttgat taatccccgt actgattatt
    13381 cttcataatc agtacggggt tgctacaaca tgtattactt tttcaccaca attaacggtt
    13441 caatatcact ctcttttttg atgaccagtg attcatcacc gcgcaaacac gtcccaccgt
    13501 agttgccgcc cacggtgaag gtacatacct gaatgtattt accgtccact ttcggcaaac
    13561 accacagttg ctgatagatg tttttctgct cggcaaattt accgctggtt ttgtccagca
    13621 cctcttcatg atggctgacc aggtcgatat tgctaccaca gcgaccggca atcggtttca
    13681 ctgcgtaacc tgttttcacc aattcatcat taacagtgaa atcggtatcc agcaggtaac
    13741 gatggtgcgg gaacagcgac cagaggatcg gcagaatcgc tttgttgccg gggatcaccg
    13801 tccacagcgg ctcaaagact agcacttccg ggcgcagcaa tacgtcgata agacgcactt
    13861 cgttttgcgg atgaccggta cggattggca ccgcagcaaa ctcacggtcg ctaacttcac
    13921 gaatctgatc aaacgcggtt tcccacgccc aggttttcca cacgcagtta accagtcgcc
    13981 cttccccatc aatcagttgc ccggcagcat cccagcccag ttcatccaat ccacgcaaga
    14041 tacgcgtttc aaagcccgcc tggtgcagcg cctgctccat aaactgcgcg tgatagtttt
    14101 cctcgatatc tttgtcctgc atgatatgga caaacggacg tgcacgactg tgtttccagg
    14161 caccagccaa ttcgttaatc agcccttccg ccggattgaa gccgttgcct ttatagccct
    14221 gctccgccca acgttcgagg atcaagcccg cttcggtatg acaggaggcg gagtcggcgt
    14281 tgtactcgta aaccttcagg ccacgctcat ccatgcagaa atccatacga ccagtgatca
    14341 tatggtgacg gcgacgctgc caggagagac gcaaacgtgg ccagaggatt ttcgggatgt
    14401 cgaacagcgc cagcaggttg tcatctttca gcaccttgtc ggttgcgtga agatacatca
    14461 ggtgcagctc gttggtggct ttaattagct cctgctcggc actctcggta atggtgtagt
    14521 aatgataagg atcctgattg atcacctgac cgttggcctg cacataggcg ttttgcagcg
    14581 gatctttttc atccagccat ttaccgtcaa actggccttt gttttccagg cgcgctccgc
    14641 tgattttcag cagctcgcct gcaatttccg gctgcggtaa gctgtattca gtatcttccg
    14701 tctggatcat ccagcccaga atggtggtgt catcaaaagt gtctttcagg gtatagcagc
    14761 cgttttcgac caccatctcc agctcacgcg tccactgttg cccttgcggc aacggggaat
    14821 gaatcacgtt ctgttccgca atacggactt tgttgccatg caattgggta atgatggcga
    14881 catggccagt gtctttaaat tcaccgcctt tatcccagat aagaagcgca cccgcgaccg
    14941 gcgcacgcgg cgagccgtta ggaaatgcct gcaatggcag gatgttgtca ttaaccactt
    15001 ctcgcaggaa gcgcagcgag aaaatctccc acgccatacc cacgtcagta aagaccacac
    15061 cgtaattcag aaagagaaaa cggcgagcaa attcaacgca ttgccacttg tggcccatat
    15121 attcgtcgtc gatatagcta cggaatacgg cgtcatcttc gtattcctgc ggatcgagag
    15181 aactgtaatc tgaagagtag attgctaccc cacctggggc gtagcccaat aatgtcccga
    15241 acggggcatc ctggctggtc gttcctttgc tcatcacttt acctttaaca atacaaccta
    15301 agcagttggc gtaattgttg tgctctgatt acctgctcag cgacattaac cggacagagg
    15361 tcagactaat catacacctc atcgcagcgg ctggcgcaaa gggttaaaaa attcacattc
    15421 tgtacagcaa gtgacctgct acactgcttc aacactacca ctcagaaggc aactcactat
    15481 gacagacaat acttatcagc ccgcgaaagt ctggacgtgg gataaatccg ctggcggcgc
    15541 gttcgccaat atcaatcgcc cggtttctgg tccgacgcat gaaaaaacgc tgcccgttgg
    15601 caaacaccca ttgcaacttt attcgctggg aacgccgaac ggtcagaaag taacgattat
    15661 gcttgaggag ctgctggcgc ggggcgttac tggtgcagag tacgacgcct ggctgattcg
    15721 tattggcgat ggcgatcaat tctccagcgg ctttgtcgaa gtgaacccaa actcgaagat
    15781 cccggcgctg cgcgatcata cgcataatcc gccgatccgc gtgtttgaat ctggttcgat
    15841 cctgctttat ctggctgaga aatttggcta cttcctgccg caggatttgg caaagcgtac
    15901 tgaaacgatg aactggctgt tctggttaca gggcgcggca ccgttcctcg gcggtggttt
    15961 tggtcacttt taccattacg cgccgatgaa aattgagtac gccatcaacc gctttaccat
    16021 ggaagccaaa cgtctgctcg acgtgctgga taagcaactg gcgcagcata agtttattgc
    16081 gggcgatgag tacaccattg cggatatggc gatttggccg tggtttggca atgtggtgtt
    16141 aggcggcgtg tatgatgccg cagagtttct tgatgcgggc agttataagc atgtacaacg
    16201 ctgggcgaaa gaagtaggcg aacgtccggc ggtgaaacgt gggcgtattg ttaaccgcac
    16261 caacggaccg ctgaatgagc agttgcatga gcgccatgac gccagtgatt tcgagacgaa
    16321 tacggaagat aagcgtcagg ggtaagggtt ggtgttcgtc gcagcaagcc atccaggccg
    16381 gataaggcgt tcacgccgca tccggcaatc gtgcacaatg cctgatgcga cgctgccgcg
    16441 tcttatcagg cctacgaaag ctaatcatca cgcgctggta atgtcgtaat ccatttggcg
    16501 cagtgcgtct aatgtggctt tggcttcatc ttcgtcgatg atgctcatgg caaatccgac
    16561 gtgtaccagc acccactggc ccagcagatc ggcagggtta ccttcacaaa tcagggcgat
    16621 attcacgtcg cgcttgatac cacatacttc aacctgcgca agctggtgaa tatcttcacc
    16681 gacagccagc acctggcctg gaacgccaat acacattatt aactccggtt attcaacttc
    16741 aatacttttg acgatcagcg aatcgccggt atcgacacgc aaccgctcgc catgacagat
    16801 cggacactgc gcatcgtgct gatgaatctc caccacctgg ctgcaatccc agcaccaggc
    16861 ctgggcgggt ttatagacga tatgtaaatc gcacccttgc gccaccgttc cgtggcagac
    16921 aatttcaaaa ctaaaacgga cggcgctctc ctcaacgcag gagagcgcgc caatttccag
    16981 ccacacggcg gtgacgcgct taacatcgtg ctgctccgcc tgccgttgga taatttcaac
    17041 ggcgctctgg caaagcgaca actcatgcat tttcgccact cctgcgacca aacagcaggg
    17101 cgcgacgccc tgcgtgtggt acatccggat tcgtgactgg cagcgaaagg atcattcgtg
    17161 cgcagtcatc cgtcaggcgt tggccctctt caatcgacat actgtgcgaa agcggtgaca
    17221 tcagcgaaca ggagaggtat tgcgaaacac cgtccagttc gccaacggta aaggtcatgg
    17281 tgccatacgg cagttgcaga ccaatttttt cactcacttt gcgcagcggc cagagttgat
    17341 ccgggccggg gaagataact gcactcagca tccacggggt gatcacacac cccgtccact
    17401 gaccttcgaa cagcgtaaaa tcagaaacat acatcggcat tgaaggatgc agaaaagaga
    17461 gatcgtgcat cgaacgccgg gcaatttctt caaacgctgc ctgtacttgc gccttcgggg
    17521 aggtctggaa acctgctatc tcttcagtca tgaatcgcct cccgtgggat ggcttccacg
    17581 cctgattcgc gcagcgcagc cagaacctgc tcaagcgcag gttcaatcat tgcttcaacc
    17641 gtcggcgtta agccgatgtg tggctccagc gattccggga tcacgccgac cagggtcagc
    17701 tttttcggaa actcgccggt gaagcgcagg gccgacaaga cgtcggccag gccaagctga
    17761 tgcggagaga ttttgttggt aaacaacgcc ggaacttctt catcccgcag gatcatcatc
    17821 gttcccggcg cgctcttctt cgacacaatg gcatccgcaa taatcagatg atcgcgattt
    17881 gccatgtcgc caagcagctc cattcccgcc gtgccgccat cgaggatctc aacataatcc
    17941 ggcagaatgt atcgttgctc taacgcttca acaatccgca caccgatggc ttcatcggtc
    18001 agcaaaatat tgccgacccc taagactaaa atacgcatta cagaaccttc actgaaacca
    18061 cttcgttgcc gtcggcatcc actacgtgta ccgcacaggc catgcacggg tcgaaggagt
    18121 gaatggtacg caccacttcc agcggtttat tcggatcagc aaccggtgta cccaccagcg
    18181 actgctcgta aggaccgacg tcatcattga agttacgcgg accagagttc caggttgatg
    18241 gaacaactgc ctggtagttg ctgatgatac cgtctttgat caccatccag tgagagagca
    18301 taccgcgcgg cgcttcgagg aagccaacac ctttgaactc acccgttgcc ggaatgttcg
    18361 gtttcacgaa ggtggtgtga tcgcctttgc cgatattggt gatcagtgca ctgtattggt
    18421 tttgcaggat atcctgcaat tcgcagcagt gaacggtacg accaataata cggcccagcg
    18481 tagagtgcag ttgcgccact tccagcgtgt taccagtcag tttctgataa atcgcaacga
    18541 tttcattcag tttgttctgg gtagattctc gacctgccgc cagtttcacc agcatattag
    18601 ccagcggacc cacttctacc gttttgccgt agaaagtcgg tgatttcacc caggaatatt
    18661 tcccgtcgtc agaccaacca tcataagccg gaatggtggt accttcccac ggcgcctgtg
    18721 gcgcttcgtc tttataccag gagtgcttcg cgctttcctg aatgcctttg atcaggtatt
    18781 catcggaatg agaagtgatc ggacgatacg aggacagatc cgcattctca atgtagccgc
    18841 ccgggaacag gaagctgccg tttttgctgt cggtcgggaa ttccggcacg ctcaggtagt
    18901 tcaccgcacc tttaccgcgc gtcagccatt ccgggtagaa cgcggcgata accgcggtat
    18961 caaccttata aacctgctca acaaagtcgc tcagtttgtc gatgaaagac ttgatgtaca
    19021 tcaggcgctc aaggttcaac acgcccaacc cgtcgaggtt gattgggttc gcgacaccac
    19081 ccactgccag gttctgaatg tgcggcgttt taccgcccag cagcgccacg acgcggttag
    19141 cgtcacgctg gcactccagc gcctgcaggt agtgcgctac cgcaatcagg ttcacttccg
    19201 gcggcagttt catcgctggg tgaccccagt agccattagc gaaaataccc aactgaccgc
    19261 tggcaaccag atctttgatc ttgttctgaa ctttggtgaa ctcttccggg ctgttcagat
    19321 gccaggtcga aacgcctttc agcatttcgg aggctttggt tgggtcagct tgcagtgcag
    19381 aagtgatatc cacccagtcc agcgccgaaa gctgatagaa atgaacgata tggtcatgcg
    19441 tggtgtgcgc agccagaatg atgttacgga tgtattgcgc gttaaccgga acgtcgatat
    19501 tcagcgcact ttctgccgca cgaacggaag acagcgcgtg agtggtagta catacgccac
    19561 agatacgttg cacaatcatc catgcatcgc gcggatcgcg gtttttcacg atctcttcca
    19621 tgccgcgcca catggtaccg gaagcccatg ctttcgaaac gacgccattt tcgatttcgc
    19681 aatcgatgcg taaatgcccc tcaatacggg ttaccggatc aatagtaatt ctctggctca
    19741 tgctttgctc gcctcatgac gattatgatc gttttgtttt aaaggaggaa gtatcggcag
    19801 tagacgaatg agtacgatgt aagcgcaaat ctcaatagcc acaaaaccaa tagaaatcaa
    19861 cagttcttcc caggtcggga agtaggcgta accgccgccc gggttgaatg ccaccagcga
    19921 ataggtcaga cgccaggttg cacaacctaa cagtgcgctc agtgctgaca ggaacagcat
    19981 gcgggaatca ttacgcagct tcgccacacg cagaacgacc agcgggaaga gcatcagcag
    20041 gacttcaatc cagaacatca cggagtagaa gtcaccggca aacgctaacg acagcttgtc
    20101 gcgatagata agctcgccaa agcgcagcac gatgaaaatc gccagcaaca cactgatggt
    20161 gttggtcagc ttgacgaaca ggctcttttc atccggaccg ttgccacgca gacccgcctg
    20221 caccagtgaa ccttcaaaga tgacaatcga gaagcccatg atgaacgccg tcagcagcga
    20281 gaacagcggc aacatttcat agctctgcca cagcggatgc accttgtagc ccgccgagat
    20341 catcagcgac cccattgaag actggtgcat ggtcggcagc aacgcaccga gcgcgatgat
    20401 gaagaacatc accttgttca gacgctgtag cgacaccttc cagcccagac gttcaaacag
    20461 tgccggagca aactccagag ccatcacgcc gatgtagatg gtcatacaga ccgccgtctc
    20521 gaacagtacc gagttcacgt tgaagtgacc cggaatgtag aagtacggca ggttccagta
    20581 gcgacccacg tcgatagtga tcgacaagcc acccagtgag taaccaaaca gactcgccaa
    20641 cagcgccgga cgcaccagcg gatggtattg cccacggtta aagacgtata ccgcccacgc
    20701 cagcgcccag ccgccacagg caaagccagt gccgatcagc aggtcaaacg cgatccacac
    20761 gccccacgga aagccgccgt tcaggtcgga gacagagccc agaccgaaca ccagacgctt
    20821 cacaatcagg agcatacaga tgacgattaa cggtccaaaa atcatgaccg gtttactgat
    20881 gattttgccg cccagcggtt ttggatcatg actcatgatc gtctcctccg tcgtgatggt
    20941 cgtttttggt gttgcgacga accagcacgg ttaagcccgc cagcacagcc agtggtagca
    21001 tcatgccttt atacagggtg tgttgaatat tttcggaacg cgcaccggta gaaagatcgt
    21061 ccagtttcgg caggtcgaga ttttcataag gcacacccgt cagtaccaga acctgagtac
    21121 cgccgccctc tttctcgccg tacagatgcg gataatattt cggcaccgta tgcaggtaag
    21181 tgtcgccaga tttcagcgtc tgacgcggat agtggtattc gctgccaggc ttcagcgcca
    21241 gacgtttttt cgcctccgcc atcagctctt cacgcgtacc gaaaatcacc gcgcccgccg
    21301 ggcacacttc tacgcagcca ggcagaccgc ctttatcgag acgttccaca cctttctggt
    21361 tgcacagctc gcacttatgc agcgcaccaa acgggttgtt gtagtcgtac ttcggcacgt
    21421 tgtacggaca ggcgaccatg cagtaacggc aaccggtgca cacgtctttg tcgtaatgga
    21481 caatgccggt tttcggatct tttttcagtg cagagaccgg gcacacagag acacagttcg
    21541 gatcgacgca gtgcatacac tgtttcttaa tgtacgcgta gccgttctcc tcctggtctt
    21601 tgttgacccc tgtgccgctg gtccacacct gaatgatgtt attggtatac ggcgacagtt
    21661 tgtcgttgtt cgaccaggtc tgttcccctt gcgggttacg ttcagggaaa ttgatatcct
    21721 gacacttggt gacgcaagcc tggcagccta cgcacaaggt cgagtcgtac aacatcccca
    21781 gcgatcccgg aattggcggg cggttttcag cagccgcatg actgacagac ggcagcgcgc
    21841 ccgtcagcaa tgccccgcag gaggctgctt taataaaatt acgtctgttc acggttattc
    21901 tccccgtgag tcagcgttat ctttcttttg ctgacgaccc agttcacgca ccgccatcac
    21961 gctgacaccg gcaaccaacc caaccacacc gccgagcaaa ccaatagcgc ctgcagagac
    22021 gttgccgccc tctttagcgt taacatccgg tttctgtgaa cgcggagttt gattttcgac
    22081 gttggcaagc tgatggatgc ctttatggaa gccgatacct tcttcgttac agccatagca
    22141 cgggtgacca atcgccaccg gccacacacc gccaacatcg cagaattgca gcgttgagca
    22201 gttgccgtaa gtttctggcc ctttacagcc gaggtggtac aggcaccagc cttcgcggtg
    22261 gccttcatca ccgaactctt tggcaaaacg accagcatcg aagtgcgggc gacgttcgca
    22321 gtgttcgtga atcagacggc cataggcgaa ggtcggacgg tttttgtcat ccagtttcgg
    22381 cggtttgccg taagtgatga tgtgcgcaac ggtcgcgagg aagttgtgcg ggttcggcgg
    22441 gcagcccgga atattgataa cggttttgcc tggcagaact tcttgcaggc tgactgcgcc
    22501 agttgggtta actccagctg cggcaacacc gccccacgca gagcaggaac cgatagcgat
    22561 aatggctgct gcgccttccg ccgctttgcg gatgtgatcc acaataggct caccggcaac
    22621 catgcaataa ataccgttat cttttaatgg gatggaacca tccaccacta acacatactg
    22681 ccctttgtac ttctcgagag cgttatgttt gttctcttcg acctgatgac cgaaggcggc
    22741 ggaaagcact tcgtgatact ccagagagat agtctccagt acgaggtttt ctaccgttgg
    22801 atgcgttgca cgaagcagag attccgtaca accggtgcac tcctgcgcgc caatccagat
    22861 aactggcgga cgctgcgggt tagtaaccga ttcggccatc tctgcagcgg ctttgctact
    22921 taaccccatg gtggcggcta atgctgcaca aagcttcatg aaatcacgac ggttaatgcc
    22981 gtgagaatgg atgagggtgt tatctccagt catttatagt tattccgttg cgaagacctg
    23041 gcatatattt tgcctcaatc gcaaaatcaa taatgcgatc gatgcgccat ttaccacaca
    23101 tttattatgg ttatcgatat catgatactg cggcggggcg ctaaaacgaa gggaagaagc
    23161 attagtgtaa ttaattaaag caagataata cgtatgtttg atcaattttc gaggcgataa
    23221 ccggccataa aaaagcgcaa cgtagaacag gaattattct cacgaaagaa atccattacc
    23281 atctctttat ccagtccata gcggcgcgtc agaatacccg cttcccaggc gctaagctgg
    23341 cgatctccgg tcttttcgta catccggtgt gagtagccta atacaaaacc gcgtttataa
    23401 tcggcacaga attttgtcac attaaccgca ctgtcggcat gcgttgcttt taatcccgcc
    23461 attaagcctt taccaaaatg gttattcatc gttcacctca ttcgctatat attgtcatat
    23521 atagcgatat tttaggcaaa gaacgacttt tgcgcacgct attttttcat gcttgtttaa
    23581 gacgtaatcc cgtaagagat aaacctatca ggagagatat ttatcgaacc atttcagtgt
    23641 cctttgccag gcaagatcgg cggcagattt gtcataacgg ggcgtggaat cattatggaa
    23701 tccgtgatta acccccggat agatatacgc ctcataaacc ttattattgg ctttcaacgc
    23761 cgcctcgtaa gcaggccagc cctcgttgat tcgggtgtcc agttccgcga agtggagtag
    23821 taaaggcgct tcaatcttcg ccacatcggc agtgggtgcc tgacgaccat aaaacggcac
    23881 cgcgcaggcc agttccggat acgcgacagc cgccgcgttc gatacgccac cgccatagca
    23941 aaatccggta atacccactt tgcctgccgc ttgcggatag cgttgcataa actcaattgc
    24001 ggcaaagaaa tcattcatca gtttggttgg atcaacctgc tgttgcagct cacgaccttt
    24061 atcatcatta cccggataac ctccaacgga acttaagccg tcaggtgcca gggcgatata
    24121 ccccgccttc gccactcgcc gtgccacatc ttcgatatac ggattcagtc cacgattctc
    24181 atgcaccacc accacggctg gcgttttgcc gctcatcctt gcgggtttca ccagataacc
    24241 ccgtacctcg ccgtgaccat ttggcgaagg atacgtgatg tactcagcaa caatctccgg
    24301 gtcggtaaac tctacctgag tcgccagcgc ataatttggc ttgagcaaat caaacaacgc
    24361 taatgccgtc atcccgccta ccgcatactt cgccgcaagg ttgaggaact cacgtttcga
    24421 gattttcccg tgagcgtaat agtcgtagta atccaacaac tcttgtggga aatctttggc
    24481 ggttaaacgc ggcatcgttg cactcctcaa ctggtgtttt tttaagcaaa gcataagcac
    24541 gtgttttcgc ccagtttttc gtcactctgt gagccagact acgggatacg cgctggcgaa
    24601 tcgctaaact agaaacattg tttcgaaatt gaacggtgga aaggagaggt catggtctgg
    24661 ttagcgaatc ccgaacgtta cgggcagatg cagtaccgct attgcggaaa aagcggttta
    24721 cgcctgcccg cgttatcgct cggtttatgg cacaatttcg gtcacgttaa cgcgctggaa
    24781 tcacagcgtg cgatcctgcg taaagcgttt gatttgggca ttacgcactt tgatttagcc
    24841 aacaattacg ggccgcctcc aggaagcgca gaagagaact ttggtcgcct gctgcgggag
    24901 gattttgccg cttatcgcga tgaactgatt atctctacca aggctggcta cgatatgtgg
    24961 cccggcccgt acggctctgg cggttcacgt aaatacctgc tcgccagcct cgaccaaagc
    25021 ctgaagcgta tggggcttga gtatgtcgat atcttttact ctcatcgcgt cgatgaaaat
    25081 acgccgatgg aagaaaccgc ctctgcgctg gctcatgcgg tacaaagcgg taaggcgctg
    25141 tatgtcggga tctcctctta ctcgccagag cggacgcaaa aaatggtcga gttgctgcac
    25201 gagtggaaaa ttccgctttt aattcatcaa ccttcgtaca atttactgaa ccgctgggtg
    25261 gataaaagcg gcctgctgga taccctgcaa aataacggcg tgggctgtat tgcctttact
    25321 cctctggctc agggattgct gaccggaaaa tatctcaacg gcattccgca agattcacgg
    25381 atgcatcgtg aagggaataa agttcgtggt ctgacgccga aaatgcttac cgaagccaac
    25441 ctcaacagcc tgcgcttatt gaatgaaatg gcacagcagc gtggacaatc aatggcgcaa
    25501 atggcgttaa gctggttgct gaaagatgat cgcgtgacgt cggtattgat tggtgccagc
    25561 cgcgcggagc aactagagga gaacgtgcag gcgctgaata atctgacatt tagcaccgag
    25621 gagctggcgc agattgatca gcatatcgcc gatggcgagc tgaatctgtg gcaggcgtcg
    25681 tccgataaat gacctgttaa taacgggcgc gcgaaccgcg cccgaatcag atcagtgatt
    25741 atgacgagtc agtcggtcaa gatagcccat cacaaatgca gaaagcacaa acgtcagatg
    25801 gataatgacg taccacatca gtttgttatc cgggacattt ttcgcatcca taaagacgcg
    25861 cagtaggtga atggaagaaa ttgccacaat cgacgctgct actttgtttt tcagcgacgt
    25921 tgcgtccatt ttccccagcc agttcagctt ctctttgttc tcggagatat ccagctgtga
    25981 gacgaaattc tcataaccgg aaaacatcac catcaccagc aaaccgccaa ccagcgtcat
    26041 atccaccagc gacagcaaca cgaggatcaa atctgattcc gccatcgaga agatattcgg
    26101 cagtacgtga ataatctcct ggaagaactt cagcgccagg gcaactaacg ccagcgaaag
    26161 gccaaagtac acgggggcaa gcagccagcg agaagcatac attgcatttt caagaaaacg
    26221 ttccatacat tcctgtcgtg tagttaaaac gggcaacagt atatcgcaat tgagtaaaca
    26281 gcacgcaaca gcaaatcaac atatacgtta aacctttgtc gcataattct gaggtttagg
    26341 gatcttcctg gttcccttat caacgcatcg agaacgtcta cccttaaagg acgttgaacc
    26401 tgaagggaga aaaacgatgt ctcatttaaa agacccgacc acgcagtatt acactggtga
    26461 atatcccaaa cagaaacaac cgacgccagg catccaggcg aagatgacac cggtaccgga
    26521 ttgcggcgag aaaacctatg ttggtagcgg tcgcctgaaa gatcgtaaag cactggtgac
    26581 agggggcgat tccggaatag gtcgcgctgc cgccatcgct tacgcgcgtg aaggggctga
    26641 cgtggcgatc agttatcttc ccgtggaaga agaagacgct caggatgtga aaaagatcat
    26701 tgaagaatgc ggacgcaaag ccgttctgct gccaggcgat ttaagcgatg agaaattcgc
    26761 ccgttcgctg gttcacgaag cgcataaggc gttaggcggg ctggatatta tggcgctggt
    26821 cgccgggaaa caggttgcca ttcctgatat tgcagacctc accagcgaac agtttcaaaa
    26881 gacctttgcc attaacgttt tcgcgctatt ctggctaacc caggaagcga tccccctgct
    26941 accgaaaggt gcaagtatca tcaccacttc gtcaatccag gcataccagc caagtccgca
    27001 tttactggac tatgcggcta cgaaggcggc gattctgaac tacagccgtg gcctggcaaa
    27061 acaggtcgcg gagaaaggta ttcgggtgaa tattgtcgcg ccaggcccga tctggacagc
    27121 actgcaaatt tccggcggac aaacgcagga taagatcccg cagtttggtc agcaaacgcc
    27181 gatgaagcgt gcggggcaac cggcggaact ggcccctgta tatgtttatc tggcaagtca
    27241 ggagtcgagc tacgtcaccg cagaagtgca cggtgtgtgc ggcggcgagc atttaggtta
    27301 aaaaaatgcc cggttgtgaa aagcaaccgg gcatcatttt tatttcgctt tggcggtttc
    27361 ttcgccgacc agacctatct tcaggtaacc cgcctgatgc agcgtatcca ttaccttcat
    27421 caacgtctcg taatcgacgg ttttatccgc tcggaagaag atggtggtgt ctttcttgcc
    27481 ttcggttaac gcattcaacg ccgtaatcat tgtttcatcg gtgaccggat cgttaccgat
    27541 aaacatcgag ttgtctgcct tcaccgacag ataaaccggt ttttccgggc gcggctgcgg
    27601 cgtgctggta gaagcaggca aattcacctt cacatctacc gtcgctaacg gtgccgccac
    27661 cataaagata atcagcagaa ccaacatcac gtcgataaac ggcgtcacgt tgatatcatg
    27721 catttcgccg ttatcatcga ggttttcgtt aagatgcatt gccatcggac attatcctgc
    27781 gcgtaatttt tgtgcgacac gaaccggatg cgcagcggcg ctggcttcca gatccaggtc
    27841 acggctttgc agcaacaata cctgcgctgc aacatcaccc agcatcgctt taaagccgcc
    27901 aatctggcgt gcaaatacgt tatagataac gaccgcagga atcgctgcca cgaggccgat
    27961 tgccgttgct aacagagctt ctgcgatacc cggcgcaacg actgccaggt tagtggtctg
    28021 cgtttgcgcg ataccaataa agctgttcat gatgccccag accgtaccaa acagaccaac
    28081 gaacggagaa atcgcgccga tggttgccag atagccgtta ccgcggccca tttgacgacc
    28141 cactgcggcg acccgacgct ccagacggaa gctggtacgt tctttaatac cttcgttatc
    28201 gtcgctgcct tctgacagtt ccagctcatt ctgcgcttca ttgagcaaat gcaggcttaa
    28261 gcttttgcta ccaaaatcag cggcgatatc gttggcctgg tttaaggaac gcgcttcagc
    28321 cagcagttgc tgctcgcgct taaggcgacg cttctgattg aagaactcta cgctcttact
    28381 gaagaagatt gcccaggtga ctacggaggc caaaataagc ccaatcatca cgcacttaac
    28441 gacaatatcg gcgtgctgat acatacccca gacggaaagg tccgtctgca ttaaattatt
    28501 acccactgtg tatctccagg acgcaagtca caaaatctgc gcataataat atcaaaacga
    28561 cgtcgaattg atagtcgttc tcattactat ttgcatactg ccgtaccttt gctttctttt
    28621 ccttgcgttt acgcagtaaa aaagtcacca gcacgccatt tgcgaaaatt ttctgcttta
    28681 tgccaattct tcaggatgcg cccgcgaata ttcatgctag tttagacatc cagacgtata
    28741 aaaacaggaa tcccgacatg gcggacaaaa agcttgatac tcaactggtg aatgcaggac
    28801 gcagcaaaaa atacactctc ggcgcggtaa atagcgtgat tcagcgcgct tcttcgctgg
    28861 tctttgacag tgtggaagcc aaaaagcacg cgacgcgcaa tcgcgccaat ggcgagttgt
    28921 tctatggacg gcgcggaacg ttaacccatt tctccttaca acaagcgatg tgtgaactgg
    28981 aaggtggcgc aggctgcgtg ctatttccct gcggggcggc agcggttgct aattccattc
    29041 ttgcttttgt cgaacagggc gatcatgtgt tgatgaccaa caccgcctat gaaccgagtc
    29101 aggatttctg tagcaaaatc ctcagcaaac tgggcgtaac gacatcgtgg tttgatccgc
    29161 tgattggtgc cgatatcgtt aagcatctgc agccaaacac taaaatcgtg tttctggaat
    29221 cgccaggctc catcaccatg gaagtccacg acgttccggc gattgttgcc gccgtgcgca
    29281 gtgtggtccc ggatgccatc attatgatcg acaacacctg ggcagccggt gtgctgttta
    29341 aggcgctgga ttttggcatc gatgtttcca ttcaggccgc taccaaatat ctggttgggc
    29401 attcagatgc gatgattggc actgccgtgt gcaatgcccg ttgctgggag cagctgcggg
    29461 agaatgccta tctgatgggc cagatggtcg atgccgatac cgcctatata accagccgtg
    29521 gcctgcgcac attaggtgtg cgtttgcgtc aacatcatga aagcagtctg aaagtggctg
    29581 aatggctggc agaacatccg caagttgcgc gagttaacca ccctgctctg cctggcagta
    29641 aaggccacga attctggaaa cgagacttta caggcagcag cgggctattt tcctttgtgc
    29701 ttaagaaaaa actcagtaat gaagagctgg cgaactatct ggataacttc agtttattca
    29761 gcatggccta ctcgtggggc gggtatgaat cgttgatcct ggcgaatcaa ccagaacata
    29821 tcgccgccat tcgcccacaa ggcgagatcg attttagcgg gaccttgatt cgcctgcata
    29881 ttggtctgga agatgtcgac gatctgattg ccgatctgga cgccggtttt gcgcgaattg
    29941 tataacattg ccacttttgg acaattttgc agacatttaa ttgtgaaaag tcttaaattg
    30001 ttgcgtccgg gatcaaggcg tcccggacga atcaggagta caataggcaa ataaaggctt
    30061 aaacgctgtt ccacaggaaa gtccatggct gttattcaag atatcatcgc tgcgctctgg
    30121 caacacgact ttgccgcgct ggcggatcct catattgtta gcgttgttta ctttgtcatg
    30181 tttgccacgc tgtttttaga aaacggcctg ctgcccgcct catttttgcc aggcgacagc
    30241 ttgttgatac tggcaggcgc attgattgct cagggggtta tggattttct gcctacgatt
    30301 gcgattctga ccgccgcagc aagtctgggc tgctggctaa gttatattca ggggcgctgg
    30361 ttagggaata ccaaaacggt gaaaggctgg ctggcacagc ttcctgctaa atatcaccag
    30421 cgcgccacct gcatgtttga ccgccacggt ctgctggcgc tgctggctgg acgttttctt
    30481 gcatttgtcc gtacgctgct gccaaccatg gcgggaattt ccggtctgcc aaaccgccgc
    30541 ttccagtttt tcaactggtt aagtggattg ctgtgggtca gcgtggtaac cagttttggc
    30601 tatgccttaa gtatgattcc gttcgttaaa cgccatgaag atcaggtaat gacgttcctg
    30661 atgatcctgc caattgcctt gttaaccgct ggcttgttag gcacgctgtt tgtggtgatt
    30721 aaaaaaaaat actgtaacgc ctgacgattt tccccgttcc cggctgctgt accgggaacg
    30781 tatttaattc ccctgcatcg cccgcattct tgccgcatct tcccccggcg tcacaccgaa
    30841 gtaacgttta aactcacggc taaattgcga tgcgctttca tagccgacgc gcatcgctgc
    30901 tgcgctagcc ttcatgccgt catggatgat catcatccgc gccttatgca gacggtaatt
    30961 cttcaaatac tgcaacggcg aggtgctggt gacagactta aaattatggt ggaacgccga
    31021 tacgctcatg ttggcttctg ccgccagttg ctcgacgctc aggttttcgg tgtatttatt
    31081 ctcaatccgt ttcagcacgc ggctaatcag actgaagtga gtctggcgac tgaccagcgc
    31141 cagtaacgcg ccgccgcaag gtccggtcag cacgtagtac agaatttcgc ggatgatctg
    31201 tttgccgaga atacgcgcat ccagtggtcg ctccatcacg tcgagtaacc gctccgccgc
    31261 gcataaaatc tcttctgata acgtggcgga gttaatcccg ctggctgcca tcgacggctg
    31321 gaaatgctca tcttcgccaa tgtccatcaa cagttcctgt aactgcaaaa tatcgacatt
    31381 gagacgcaac cctgccagcg gcacctctga cgtggcatag gtttcgcact caaacggcaa
    31441 cggcaccgtc agcagcaggt attcattggc atcataacga aacacgcgtt cattgatata
    31501 accgatttta tgcccggaaa agagaattat gatgccaggc tcgtacatca ccggtgtacg
    31561 tgcgaaaggc gtctcgccat acaacaaacg cacatcgggc aacagttctg acaaactatt
    31621 ttctttattt ttcagtttat taactttatc cgccagcaag cggcaaatct cttcacgttt
    31681 catatcgcgt aatttcttag gaataatgcg gcaatttgat tgtgcgcaat tttgtagcat
    31741 ttctccagca ctctggagaa ataggcaaga cattggcaga aatgagcatt gagagccagg
    31801 gcgctggcga tcacaatgaa aaacatcagg cagatcgttc tctgccctca tattggccca
    31861 gcaaagggag caagtaatga acaactttaa tctgcacacc ccaacccgca ttctgtttgg
    31921 taaaggcgca atcgctggtt tacgcgaaca aattcctcac gatgctcgcg tattgattac
    31981 ctacggcggc ggcagcgtga aaaaaaccgg cgttctcgat caagttctgg atgccctgaa
    32041 aggcatggac gtgctggaat ttggcggtat tgagccaaac ccggcttatg aaacgctgat
    32101 gaacgccgtg aaactggttc gcgaacagaa agtgactttc ctgctggcgg ttggcggcgg
    32161 ttctgtactg gacggcacca aatttatcgc cgcagcggct aactatccgg aaaatatcga
    32221 tccgtggcac attctgcaaa cgggcggtaa agagattaaa agcgccatcc cgatgggctg
    32281 tgtgctgacg ctgccagcaa ccggttcaga atccaacgca ggcgcggtga tctcccgtaa
    32341 aaccacaggc gacaagcagg cgttccattc tgcccatgtt cagccggtat ttgccgtgct
    32401 cgatccggtt tatacctaca ccctgccgcc gcgtcaggtg gctaacggcg tagtggacgc
    32461 ctttgtacac accgtggaac agtatgttac caaaccggtt gatgccaaaa ttcaggaccg
    32521 tttcgcagaa ggcattttgc tgacgctaat cgaagatggt ccgaaagccc tgaaagagcc
    32581 agaaaactac gatgtgcgcg ccaacgtcat gtgggcggcg actcaggcgc tgaacggttt
    32641 gattggcgct ggcgtaccgc aggactgggc aacgcatatg ctgggccacg aactgactgc
    32701 gatgcacggt ctggatcacg cgcaaacact ggctatcgtc ctgcctgcac tgtggaatga
    32761 aaaacgcgag accaagcgcg ctaagctgct gcaatatgct gaacgcgtct ggaacatcac
    32821 tgaaggttcc gacgatgagc gtattgacgc cgcgattgcc gcaacccgca atttctttga
    32881 gcaattaggc gtgccgaccc acctctccga ctacggtctg gacggcagct ccatcccggc
    32941 tttgctgaaa aaactggaag agcacggcat gacccaactg ggcgaaaatc atgacattac
    33001 gctggatgtc agccgccgta tatacgaagc cgcccgctaa gctttttacg cctcaaactt
    33061 tcgttttcgg gcatttcgtc cagacttaag ttcacaacac ctcaccggag cctgctccgg
    33121 tgagttcata taaaggagga acgtatggct aatccaaccg ttattaagct acaggatggc
    33181 aatgtcatgc cccagttggg actgggcgtc tggcaagcaa gtaatgagga agtaatcacc
    33241 gccattcaaa aagcgttaga agtgggttat cgctcgattg ataccgccgc ggcctacaag
    33301 aacgaagaag gtgtcggcaa agccctgaaa aatgcctcag tcaacagaga agaactgttc
    33361 atcaccacta agctgtggaa cgacgaccac aagcgccccc gcgaagccct gctcgacagc
    33421 ctgaaaaaac tccagcttga ttatatcgac ctctacttaa tgcactggcc cgttcccgct
    33481 atcgaccatt atgtcgaagc atggaaaggc atgatcgaat tgcaaaaaga gggattaatc
    33541 aaaagcatcg gcgtgtgcaa cttccagatc catcacctgc aacgcctgat tgatgaaact
    33601 ggcgtgacgc ctgtgataaa ccagatcgaa cttcatccgc tgatgcaaca acgccagcta
    33661 cacgcctgga acgcgacaca caaaatccag accgaatcct ggagcccatt agcgcaagga
    33721 gggaaaggcg ttttcgatca gaaagtcatt cgcgatctgg cagataaata cggcaaaacc
    33781 ccggcgcaga ttgttatccg ctggcatctg gatagcggcc tggtggtgat cccgaaatcg
    33841 gtcacacctt cacgtattgc cgaaaacttt gatgtctggg atttccgtct cgacaaagac
    33901 gaactcggcg aaattgcaaa actcgatcag ggcaagcgtc tcggtcccga tcctgaccag
    33961 ttcggcggct aacatgcaaa ttctcccggt ggcggtaatg ttccgctacc ggacttttca
    34021 gaaatcattt attcccctcg cgtcccgccc gttgttactc ttccttgttc aggaatgcca
    34081 aatataagga catcatcatg cagagccgga agctcttaaa agaacaactc atctatatcc
    34141 gggataaacg caacggagag gtgaaaaaca gatgaaaata atacttctgt ttttagcagc
    34201 cctggcaagt tttaccgtac acgcacagcc cccctcactg accgtagaac aaacagtccg
    34261 gcatatttat cagaactata aatcagatgc cactgcccct tattttggtg aaaccggaga
    34321 gcgggcgata acttctgcgc gtattcaaca ggcgcttacc ctgaacgaca atcttacgct
    34381 gccgggcaat attggctggc tggattatga tccggtttgt gattgtcagg attttggcga
    34441 tctggtgcta gaaagcgttg cgataaccca aactgacgcc gatcatgccg atgccgttgt
    34501 gcgctttcgt atctttaaag atgataaaga aaagaccacg cagacactga aaatggtggc
    34561 ggaaaatggt cgttgggtca ttgacgatat tgtcagcaat catggcagcg tcttacaagc
    34621 agttaatagc gagaatgaaa aaacgctggc cgctttagct tcgttgcaaa aagaacagcc
    34681 ggaagccttt gttgccgaac tctttgaaca tattgctgat tatagctggc cgtggacgtg
    34741 ggtggtttcc gactcttacc gccaggcggt taatgccttc tataaaacca ccttcaagac
    34801 ggccaataat cccgatgaag atatgcaaat agaacggcaa tttatttacg acaatccgat
    34861 ctgttttggc gaagagtcgc tattttcacg cgttgatgaa attcgagtcc tggagaaaac
    34921 cgccgattcc gcccgcattc atgttcgttt tacgctgacc aatggcaaca acgaagagca
    34981 agaactggtt ttacagcggc gcgaaggcaa gtgggaaatc gctgatttta tccgcccgaa
    35041 cagcggcagc ctacttaagc agattgaggc aaaaaccgcc gccagattaa agcaatgagc
    35101 tgaattaaat aacaattagc cggaacaata aataaaaggg aacactatat gaaaacgatt
    35161 ttcaccgtgg gagctgttgt tctggcaacc tgcttgctca gtggctgcgt caatgagcaa
    35221 aaggtcaatc agctggcgag caatgtgcaa acattaaatg ccaaaatcgc ccggcttgag
    35281 caggatatga aagcactacg cccacaaatc tatgctgcca aatccgaagc taacagagcc
    35341 aatacgcgtc ttgatgctca ggactatttt gattgcctgc gctgcttgcg tatgtacgca
    35401 gaatgataaa aaaatccccg gcagcatgtc agttgccggg gatttttttt aacgtccaac
    35461 cgccgcttta gggcgtttct tcgcaccagc attcaccgga cgagattgcg tagacgacgc
    35521 tttttttgcc gtagcaggcg tctgacgctg ggtcgccatc ggcgtatgtt tcgtcaacgc
    35581 cggacgggta ttgcggttct ggcgacgagc ttcacgcatc tcttcaatgg ttggcgcagg
    35641 cactaagcaa tcgcgacggc tgccaatcag atgctttttg cccatcgctt ccagcgcctg
    35701 gcggattaac ggccagtttg ccggatcgtg gtaacgcaac aacgctttat gcaaacgacg
    35761 ctgtttgtcg cccttcggta cgaagacgtc ttcgctctta taaccaatct tcgccagcgg
    35821 gtttttcccg gtgtaataca tggtggttga gttcgccagc ggcgatgggt agaagttctg
    35881 tacctggtcg agacggaaac gatgcttttt cagccacagc gccagattca ccatatcttc
    35941 atcacgcgta ccggggtgcg cggagatgaa ataggggatc agatactgct ctttacctgc
    36001 ctgttttgag taagtatcga acagctcttt aaagcggtca tagctgccca tgcccggctt
    36061 catcatcttc gataacggcc cttcttcggt atgttccggg gcaatcttca gataaccgcc
    36121 gacgtgatgg gtcgccaact ctttgatata gcgcggatct tccacggcga tgtcataacg
    36181 tacgcctgag gcgatgagga tttttttaat gcctttcaga tcacgcgcac ggcgatagag
    36241 gttgatcgtc ggttcgtggt tagtgtccat gtgcggacaa atatccggat aaacgcacga
    36301 caaacggcga caagtttgtt cagcgcgtgg cgatttgcag cgcaacatat acatgttggc
    36361 agttggccca ccgagatcgg aaatcacgcc cgtaaaacct ggaacggtgt cgcggatcgc
    36421 ttcgatctca ttaatgatcg aatcttccga acggctctga ataatgcgcc cttcgtgctc
    36481 ggtaatagaa cagaaagaac agccgccaaa gcagccgcgc ataatgttga ccgaaaaacg
    36541 gatcatttcg taagccggaa tacgggcatt gccataggcc ggatgtggca cgcgcttgta
    36601 cggcagcgca aaaacgctgt ccatctcttc ggtagaaagc gggatagcag gcgggttgat
    36661 ccacacatag cggtcaccgt gtttttgcat caatgcgcgg gcgcagcctg ggttggtttc
    36721 gtggtgcaga atgcgcgaag catgggcgta cagcacttta tcgcccttca ctttctcgaa
    36781 agaaggcagc aacacgtagg ttttttccca cggtttcggg cgcggtggct gcacggttac
    36841 ggctttggct tcctgctttt tcggtgccac cggtttgtta tccgcgcacg gcaaatcttc
    36901 accatacgga tgcgggattg ggtcgatttt tccaggggta tcaagacggg tggaatccac
    36961 gccgctccag ccaggcagcg cctctttcac gataatcgcg gtattacgca catcgcggat
    37021 ttcactaatc ggctcgccca tcgccagacg gtgcgccacc tccaccagcg gacgctcacc
    37081 gttaccaaac atcagcatgt cggctttcga atccaccagc acggaacggc gcacggtatc
    37141 ggaccagtaa tcataatgtg cggtacggcg cagactagcc tcaataccgc cgaggatcac
    37201 cggtacatct ttccacgcct ctttacaacg ctgggtataa accagtgtgg cgcgatccgg
    37261 gcgcttaccc gcgacgttat ccggcgtgta ggcatcgtca tgacgtaaac ggcgatcggc
    37321 ggtataacgg ttgatcatcg aatccatgtt gccagcagta acaccgaaaa acagattcgg
    37381 tttacccaga cgcatgaaat cgtctttgct gctccagtct ggctgggcga tgatcccgac
    37441 gcgaaagccc tgtgcttcca gcatacgacc gcaaatcgcc atcccgaagc ttgggtgatc
    37501 gacatacgcg tcgccagtaa ccaaaatgat gtcgcagcta tcccagccaa gttgatccat
    37561 ctcttcacga gacatcggca aaaacggtgc cggtccaaaa caggcggccc agtactgcgg
    37621 ccaggagaac aggtcgcgat ccggttggat cagggagata gagctcatat tgcttccaga
    37681 aatgataaaa aaataatcaa aggccgggga ttataagccg gaacgaaaga gaaatcgaaa
    37741 ggtattccat actcgccctc ctcgggcgag tatgaagatt acggtaccgg attgaccaaa
    37801 agttgcccta tcgagccacg gtccgccatt tccagcgtct gactgttgaa gtagaacggg
    37861 aagtgcgccc aggaaggctg accgaaataa acaagcagct ccacttgtcc gtcaacccaa
    37921 acggtatctt tccagcctct gtcttccggg aacggcatcg cgccattcac gttacggatc
    37981 tggaacatta cgccttcaat atggaacgct tgcggctcgt ccgcgcgtac cgtccagcgt
    38041 tcccacgttc cttgctgcgc ggtgacatca atacggttga cgtcccacag ctgtccatta
    38101 atacccgggt catcacccag actgatatcg cgactgcgaa ttggcgaacc agccatgatt
    38161 tcagttggca gcaagcgcat cggaagactg tctgtgacca gcggcagaag gccggttggg
    38221 cgcagcgtta gcaccagggt agaaaccaga atactggatg gctcaaagaa gccgcgaata
    38281 cgatcaacaa tgctcgccgc ttcgccacag gtgatcgaca cttcatcgcc gttgctcata
    38341 tccaccagaa tctcgcggcg ctcgcccggt gccagcgaaa gttgcttcac cgatacagga
    38401 gcaggcagga atccctgatc gccagaaatc acatgtaacg ggcgaccatc gctcatctgt
    38461 agttgatagc gacgagagtt cgacgcgttc agcagtcgca agcgcaccca gccacgcgag
    38521 acttcaacgt acgggctttg tacaccgtta accagcagcg tatcaccaac aaagccgccg
    38581 cttcccggtt cgttgtattc tggcgtacca aagttatcca gccgtttatc ctggataatg
    38641 accggaaaat catccacacc ataatggttg gggataggca gcgacttgct gacttcatct
    38701 tccaccagcc acattccggc aaggccgtta tagacctgct gagccgtgcg gttgggagta
    38761 ttggcgtgat accacagagt agctgcgttc tggcgaatgg gcagtacggg tgcccagtca
    38821 gcgtttggcg acatcatccg tgccggaccg cccatcagcg ggcctggtac ctgtagcccg
    38881 gccaccgtca ttgagacatt ttctgtcagg cggttgctgt aaataagctt aacatcgtcg
    38941 cccttccaga cgcggatagt cggccccagg taacgaccat tgattcccca gaccgacgcg
    39001 cgtgtccctg gcgtaaatga ccagtgcgca cgttgtacag tcataaacag cggttgccca
    39061 cggcgagatt caagtagcgg cggaacgggt agcggttgct gttgcccggc tgcgctggcc
    39121 ttcaggggaa cagcgcctgc acaaagtgca atccccgatg cctgaatgaa ctgacgccga
    39181 ctgagtgaca tatttgctcc atgtaaaact ggctaaagta ataccgtgca ggaaagtgtt
    39241 ccccgcaaaa atacagattg ccgacttaaa cttttccggc ggcttcgcgt tctgcgactt
    39301 ctttatcgag ctcggcgatt ttttgttcca ttatcgaacg acaatgggca gccagctcac
    39361 gaacctgatc tttgccatac tgactgacgt caattggcgg cagcatttcg acaatcacca
    39421 gaccgttgtg cagtcgatta agattaatct tattcgaagt tgtagagacg cacacgggaa
    39481 taatcgggac gcccgccgca attgccgcgt gaaatgctcc agtcttgaac ggtagcaggc
    39541 cgcgaccacg gctgcgggtt ccttccggga acatccagat ggaaatacgg cgttttttga
    39601 aatgattcac gacttccgca atggtgccgt gagctttagt gcgattgttt ctgtcgatca
    39661 ataagttgcc ggttaaccag tacaactgcc cgaagaaggg gatccacagc aagctctttt
    39721 tacctaccgt caccgtcggc ggttgcacaa tgttcgatgc tgtcaccatg tcatagttgt
    39781 tctggtggtt agcgatatag atagcattgc cgtagctttc agcatctgca ggtttgcggc
    39841 actcaacttt caggccgaac agcggcgcaa gacggccaaa catatgccca aaggtggcca
    39901 catgtttcgg gttacgcggg ctgaaaaggc agtaaatgga gccgaataca cagactaaga
    39961 tgctgtaaat cacggtaata ataagacgaa agatatatag cataacaccc tctgaaggtc
    40021 tgagagactg gcattgtacg tcacctgaaa tcaggttagg gcattgttaa aagccgctct
    40081 ctggaaaagc gtattgttaa tcgcaacgca tgaataaaca acggtttcaa gggaattttt
    40141 atcgtttccg ggggttgaat acgctgccgg atgacgactt aacgtttcat ccggcgttcc
    40201 ttgcaagcgg gaggaaacag gccctccccg gcatattact cttcgctatc accgctgctg
    40261 gcacggcgag gagagtcgat ctcaacacga tcgatacgct gcaaaccgcg catcaacgta
    40321 ccgcggcgtc cacgttcgcc agtgactttc tgtaactctt ccgggcgcag tttaattttg
    40381 cgtttcccaa catgaatggt cagcgtgctt tgcggcggca gaacgtacaa ttgcgccaga
    40441 ccatcttctc cacgcgcggc ttctgccgat ggaatgttga taatcttgtt gcctttgccc
    40501 ttcgacagct gcggcagatc gcttaccggg aacatcaaca tacggcctgc ctgagtgatt
    40561 gccagcagca tatcggaagc atcttcaatc accaccggcg gcataacatg ggcattttcc
    40621 ggtaaggtga tcaaagcctt acctgcacgg ttacgcgcca ccagatcgtt aaaggtgcag
    40681 acgaaaccgt aacccgcatc ggaagccatc agcagtttct gatcgtcgct ttccatcagc
    40741 atatggtcaa cggtcgcccc aggcggcaac gttaatttgc cggtgagtgg ttcgccctga
    40801 ccacgcgccg acggcagcgt aatcgggtcg atggcatagc tacgaccggt ggaatcaaca
    40861 aacactaccg gttggttgct cttacctttc accgccgctt tgaagctatc acccgcttta
    40921 taattcaggc ccggcgcgtc gatatcatgg cctttagcgc tgcgtaccca gcccatctgc
    40981 gacagcacaa tggtgacagg ttcagacggc agcatgtcgt gctcgctcat cgctttcgct
    41041 tcttcgcgtt cctgcaacgg cgaacgacga tcgtcaccgt aggcttgcgc gtctgcctgc
    41101 agttctttct tcagcaggtt attcatttta cgctcggaag ccaaaatgcc ctgcaactgg
    41161 tcgcgctctt tttccagctc actctgctca ccgcgaatct tcatctcttc cagtttggca
    41221 agatgacgca gtttcagttc gaggatcgct tccgcctggg tttccgtaag gccaaaccgc
    41281 gacatcagcg ccggtttcgg ttcatcttcg ttacgaatga tctcaatcac ttcgtcgatg
    41341 ttgagaaacg ccaccagcaa accttccagg atatgcaggc gcttgaggac tttctccaga
    41401 cgatagttca gtcggcggcg cacggtatcg cgacggaaca ccagccattc ggagaggatt
    41461 tccagcaggt ttttcaccgc cggacgacca tccagaccga tcatattgag gttaatgcga
    41521 tagctctttt ccagatcggt ggtagcgaag aggtggttca tcacctgatc catatccacg
    41581 cggttggaac gcggcacaat caccagacgg gtcgggttct cgtggtcaga ttcatcgcgc
    41641 agatcgtcaa ccatcggcag ctttttgttg cgcatttgcg cagcaatttg ctccagtacg
    41701 cgcgcacctg aaacctgatg cggtaatgcg ctgataacca ccgcgccatc ttctttcttc
    41761 cacaccgcgc gcatacgcac tgaaccacgt ccgttctcgt agattttacg gatctcggcg
    41821 cgcgaagtga taatttccgc ttcagtcgga taatccggcc cctgcacgat atccagcagc
    41881 tgatcgagcg tggttttcgg ctggtcgatt aatgcgattg ccgcctgagc tacttcacgc
    41941 aggttatgcg gtggaatatc ggtcgccatg ccgacggcaa taccggtggt gccgttaagc
    42001 aaaatgtttg gcagacgagc aggcagcatt ttcggctcct gcaaagtgcc gtcgaagttt
    42061 ggcacccagt cagccgtccc ctgccccagc tcgctcaata gcagctcgga atatttcgac
    42121 aaccgggatt cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg cgcgccccag
    42181 ttcccctgac catcaaccag cggataacgg taagagaacg gctgcgccat caggaccatc
    42241 gcttcataac aggcgctatc gccgtgcgga tggtatttac ccagtacgtc accgacggta
    42301 cgggccgatt ttttaaattt ggcgctggca ttcaggccca gttcagacat cgcatacaca
    42361 atgcggcgct gaacaggttt aagaccatcg ccaataaacg gcaacgcacg gtccatgatc
    42421 acgtacatgg agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc aaggcgctct
    42481 gccatatcgc tcattaattc tgattcctca acttattcgc cagccttcaa aggggcaata
    42541 ttgccggaga tactacatta tctgccgcgt cgagtcacag gagatcagcg tgaatagcgc
    42601 cgccggacgt gctccgacgg cgataaaggc atcaatgtgc cttgatatac aactctttgc
    42661 tgtagacttg atcggcatag agggcagcac aaggttgcgc ccatgccaca ccactcggca
    42721 tgggggtccg ctccgggcga ttcgagaagt tgaggttatg cgagtccagg gaagcctaaa
    42781 gcatcaaagt agtcgactgt aagtatacgg ttcagcccag tttagctgtg tctccaccag
    42841 tgccccgcgc ctcctgccat aagtgcggct tgctcatgga tacccccgag acggcttacg
    42901 gcgcaccgga taataaattt cgcgtataca tggtattcct taccggagaa attataagcc
    42961 caacaacaat acgcctgtgg cattgttcgc tcaaacttat cgcgagtgat ttggctcaca
    43021 ctccggtaaa gattccccat cacgccaacg gcacataaac atccgtttcc agtcgctgct
    43081 ctgtcacccc ttcggcaaga ttggtgtagt gaaacagaat cggtgcttta cgcattttct
    43141 cgccgcttgc aggcagccag tggcgaataa tgccccatat cgtgtgtgaa atatcgtcca
    43201 gctcgccaac gtggcgggcc acggcataac gtccaccggt aagttcacca ttgctcacac
    43261 cataacgatt atcgggaatc ggttcgctaa cgctgccgca gatatcaaag cgaaacgctt
    43321 ccggtgcggt ggtggcaggg tcgtcccagg cgacgccaaa agtctggctt tggttaacag
    43381 gagaaagccc cgtctcttta cgccacatga taaatttcgc cacactgtag ttgagcaact
    43441 cagggctaca gcggtgcggc aacatcgcca cagggattga tgggaaatcg ataatgttta
    43501 catccagtgt caggtttgtc attttgcctc cctgcgaaat tattcccggg aacgcgtccc
    43561 gggagcggta acaattacgg atttactttg cggatctgtt tgacgtcaat ttcaacagaa
    43621 ttccagtctt tatcgacttc accctgaatc tcaaccgtat ctttcggcgt caccgtcaca
    43681 ccgttccagc gtttgtggtc gatatcaaca ttgatagtac cgctggcatc tttaaacacg
    43741 tagagatcgt cagagatgcg ttcaacgata ttgccgcgca gggttaccca ggtgtcgtca
    43801 cgcagggatt ttgcgctttc tacggtcgtt acgctgccgt tcggcccctg gaatcctccg
    43861 gcctgacttt gcgttgccga tgggccagaa aaaccgccct gctctgctgc catcaccggt
    43921 gcgctgcaca gggccattac tgcgattact gctgcgaatt ttttcatgtt tattactccc
    43981 tttaatgtct gtttccgagc atttaacaag atagtcctta acaacttctt aagggaaaaa
    44041 aataaaattt agtgctgtac agagcgcgtt acaacacggt ttactggcag caaatacggt
    44101 tatcgcaggg atgaaaaaat gcgaatttta ctgatagaag atgacatgct gattggcgac
    44161 ggcatcaaaa cgggccttag taaaatgggt tttagcgtcg actggtttac acaaggtcgt
    44221 cagggaaaag aggcgcttta tagcgcgcct tatgatgcgg tgatcctgga tttaacctta
    44281 ccaggcatgg atggtcgcga tattttgcgc gaatggcgag aaaaaggtca gcgtgagccg
    44341 gtactgatcc tgaccgcgcg cgatgcgctg gaggaacgtg tagaagggct gcgtctggga
    44401 gctgacgatt atctgtgtaa accttttgcg ttgatagaag tcgccgccag gctggaagct
    44461 ctgatgcgcc gaaccaacgg ccaggccagc aacgagctgc gccacggtaa cgtcatgctc
    44521 gaccccggca aacgtatcgc cacgctggct ggcgaaccct taacactgaa accaaaagaa
    44581 tttgccctgc tggaattact gatgcgtaac gctggtcggg tactgccgcg caaactgatt
    44641 gaagagaaac tgtatacctg ggacgaagag gtcaccagta atgccgttga agtgcatgtg
    44701 catcatctgc gacgcaaact cggtagtgat tttattcgta ccgtgcatgg tattggctac
    44761 acattaggtg agaaatgaaa tttacccaac gtcttagtct gcgcgtcagg ctgacgctaa
    44821 tctttttaat tctggcctcg gtgacctggc tgctttccag ctttgtcgcc tggaaacaaa
    44881 caacggataa cgtcgatgaa ttgttcgaca cccaactgat gctgtttgcc aagcggttaa
    44941 gtacgctcga tctcaacgaa atcaacgcgg cggatcgcat ggcacagacg ccaaataaat
    45001 taaaacacgg tcatgttgat gacgatgcgc tgacctttgc catctttacc cacgacggca
    45061 gaatggtcct taacgatggc gataacggag aagatattcc ttatagctat caacgggaag
    45121 gttttgctga cgggcaactg gtgggcgata aagatcaatg gcgttttgtc tggatgacct
    45181 cacctgatgg caaatatcgc atcgttgttg gtcaggaatg ggaataccgt gaagacatgg
    45241 cgctggcgat tgttgccggg caattgatcc cgtggctggt cgcactgccg attatgttaa
    45301 tcatcatgat ggtactattg ggtcgtgaac tcgcgccgct gaacaaactg gcgctggcgc
    45361 tgcgtatgcg tgaccctgac tcggaaaaac cactaaacgc aactggcgta cccagcgaag
    45421 tgcgtccgtt ggttgagtcg ctaaatcaac tgttcgcccg cacacatgcg atgatggttc
    45481 gtgaacgacg ctttacctcc gacgcagctc acgaactacg tagcccgtta acggcgctga
    45541 aagtgcaaac cgaagttgcg cagctttctg acgatgatcc gcaagcgcgg aaaaaagcgc
    45601 tgcttcaatt acattacggg atcgatcgcg ctactcgtct ggttgatcaa ctgctcactc
    45661 tatcgcggct ggactcactg gataaccttc aggacgtcgc ggagatcccg cttgaagatc
    45721 tcctgcaatc gtcggtgatg gatatttacc acacggcgca gcaggcgaaa attgacgtgc
    45781 gactgacact caatgcccac ggcatcaaac gcaccggaca accgctattg ctaagtttgt
    45841 tggtgcgaaa tttgctggat aacgccgtgc gctacagtcc acagggcagc gtgatagacg
    45901 tcacgctgaa tgctgataat ttcatcgtga gggataacgg ccccggtgtg acgccagagg
    45961 ctctggcgcg aattggcgaa cgcttctatc gcccccccgg acaaaccgct accggcagtg
    46021 gacttgggct atcgattgtc cagcgaatcg ccaaattgca tggcatgaat gttgagtttg
    46081 ggaatgcgga acaaggtgga tttgaggcga aggtaagctg gtaacttctc tgtctatgag
    46141 agccgttaaa acgactctca tagattttac taatagcaaa atataaaccg tccccaaaaa
    46201 agccaccaac cacaaaccag acaaataccg agcgaaaata catataccca aaactcgaac
    46261 atttcccgca taaagagttt tcttaagata agaataataa gtggcgtaag aagaaaaaat
    46321 gctgcaatca ttgcttgcaa tatacgatct tcgcctgaaa cggaaggtat actgcaaata
    46381 ttgtctcccg actcaagcat ccagtcatac ttattgaaag caaaaaaagc atctattaac
    46441 caataagtaa gcattatgta taacagagct tcaaatattg tttgaatttt atgctttaac
    46501 atatatcagt acgctcataa aaatccattt ataattatca acatgacacg ttgatatttt
    46561 catcttttac ttattcaaag ccatattttc aatcccaaaa aaaaccgaac caaacgtgta
    46621 aattattccg gtgcgagtaa gaattatgag gaatggctat cagtattgtc attttcagaa
    46681 aatatttatc ctgcatcggt gagtcagagc aagatcagac ttttgctaaa ttcgcaaaag
    46741 actttgcaca ttttgctaat ttcaccgtac cgctctgtga cgtattatag tcggcaaacg
    46801 tctcaccttg aggttaaaaa atgagcaaca tcctgattat caacggcgcg aaaaaattcg
    46861 cccactccaa tggtcaactg aacgacaccc tgaccgaagt cgcggatggc acactgcgcg
    46921 acctcgggca tgatgtccgc atcgttcgcg ccgacagcga ctacgatgtc aaagcggaag
    46981 tacaaaactt tctctgggct gatgtggtga tctggcagat gccaggctgg tggatgggcg
    47041 cgccgtggac agtgaaaaaa tatattgatg atgtattcac cgaaggtcat ggaacgctgt
    47101 atgccagcga tggccgtacc cgcaaagatc cgtcaaaaaa atacggttcc ggcggcctgg
    47161 tacagggcaa aaaatatatg ctttctctga cctggaacgc accaatggaa gccttcaccg
    47221 aaaaagatca attcttccac ggcgttggcg tcgacggtgt gtatctgccg ttccataaag
    47281 caaaccaatt ccttggcatg gaaccgctgc cgacatttat cgctaatgac gtgataaaaa
    47341 tgcctgatgt tccccgctat actgaagaat atcgcaagca tcttgtggaa atttttggtt
    47401 aactagagct caggctttag aaggagttaa ccatgcttac cgtaatcgca gaaatccgta
    47461 ctcgtcctgg tcaacatcac cgtcaggcgg tattggatca gtttgctaaa atcgttccaa
    47521 ccgtactgaa agaagaaggt tgccacggct atgcgccaat ggtggattgc gctgctggtg
    47581 tgagtttcca gtctatggca ccggattcta tcgtgatgat tgagcagtgg gaaagcatcg
    47641 cgcatcttga agcgcatctg caaaccccgc acatgaaggc gtacagcgaa gccgtaaaag
    47701 gcgacgtgct ggagatgaat atccgcatcc tgcaacctgg gatttaaacc tgccttgttt
    47761 gcccggccat cctgaccggg caatgttctt tcctttaaac ctcaatctcc gccatgtcgc
    47821 ctttctcttg caaccagttg cggcgatctt ccgagcgttt cttcgccagc agcatatcca
    47881 tcatcgcgtc agtacgctga tcatcttcat catcgatagt caactgcacc agacggcgag
    47941 tgttcggatc aagcgtggtt tcacgcaatt gcatcgggtt catttccccc agacctttaa
    48001 aacgctggac gttcggcttg cctttcttgc gttttaattg ctcaagtacg ccctctttct
    48061 cttcttccgt cagcgcgtaa taaacctctt tcccgagatc aatacggtag agcggtggca
    48121 gtgcgacgta aacgtgaccg tgtttcacca acgcgcggaa gtgttttacg aacaaagcgc
    48181 agagcagcgt ggcaatgtgc agaccatcag agtccgcatc cgcgaggata cagattttgc
    48241 cataacgaag ctggctcaga tcgtcgctgt caggatcgat accgatcgct accgaaatat
    48301 catgcacttc ctgcgaggcc agcacttcgt cggaagaaac ttcccaggtg ttaaggatct
    48361 tacctttcag tggcatgatc gcctgatatt cgcgatcgcg cgcctgcttg gcagatccgc
    48421 ctgcggagtc accttccaca aggaacagtt cggtacggtt aagatcctgc gcggtacagt
    48481 cagccagttt gccaggcagt gctgggccgc tggtcagctt tttgcgcacc acttttttag
    48541 ccgcacgcat acggtgctgg gcgctggaaa tcgccatctc cgccagcagc tccgccgcct
    48601 gaacgttctg gttcagccac aggataaagg catctttcac cacgcctgaa acgaatgccg
    48661 cgcactgacg cgaagagaga cgctctttgg tctgcccggc aaactgcgga tcctgcattt
    48721 ttactgacag cacataggcg cagcgatccc agatatcttc cgccgacaac tttacaccgc
    48781 gcggcagaat gttgcggtat tcacagaact cacgcatcgc gtccaacagg ccctgacgca
    48841 gaccgttaac gtgcgtaccg ccctgcatcg ttgggataag gttgacgtag ctttcggtca
    48901 gcagttcacc gccttccggc agccacagta gcgcccagtc cacagcttca gtatcaccag
    48961 cgaaattacc gataaacggt ttttctggca gcgtcggtaa accgtttacc gcttccgcca
    49021 ggtaatcatt cagaccgtcc tggtagcacc agcgctgttc ggtgttgttg atctcatctt
    49081 taaaggtaat ctcaacgcca gggcacagta ctgctttggc cttcagcaca tgcgtcaggc
    49141 gtgaaacaga aaaacgcggg ctgtcaaaga aggtttcatc cggccagaag tgcacgctgg
    49201 taccagtatt gcgtttaccg caagtgccga caacctgtaa atcctgcacc ttttcgccat
    49261 tttcaaaggc gatgttataa acctgaccat cgcggcgcac gttaacttct acgcgcttcg
    49321 acagggcgtt aaccaccgaa atccccacgc catgcaggcc gccagagaac tggtaatttt
    49381 tgttagagaa tttaccgcct gcatgcagac ggcaaagaat cagttcaacc gccggtacac
    49441 cctcttccgg gtgaatatcc accggcatcc cgcgcccatc gtcaataact tctaacgact
    49501 ggtcagcatg taagattaca tccacgcgtt ttgcgtgacc cgccagtgct tcatcgacac
    49561 tgttatcaat aacttcttgc cccaaatggt tagggcgagt ggtatcggta tacatccccg
    49621 gacggcggcg aaccggctca agcccggtga gtacctcaat ggcatcagcg ttataagttt
    49681 gcgtcatggt ttaagttagc aattcgagtt gatcgtcaga gatggtgcag accaagaaaa
    49741 tcgacgatcg ggttgaaata atcttcgaag cccgtgaatg cgtggttgcc gccttctatg
    49801 acagtctggc ggcaggaagc gtagtacgcc accgcctggc ggtaatccag cacttcatct
    49861 cccgtctgtt gcagcagcca gatcaaatcc ggcgcttcca gcgggtcaat ctgcatgact
    49921 ttgagatcgt aaatatggcg tgactctagc acatattgct gcccggtgta ggggttctcg
    49981 ttctgaccga gatagtccgt cagcagttca aacgggcgca ccgccgggtt taccaccact
    50041 gcgggcagca taaaacattg tgacaaccag gtggcgtaat atccccccag tgacgaaccg
    50101 acaataccca gcgaatcgcc gccatgttcc aggacaatgg attccagcag ctctgccgcg
    50161 tcggaaggat acggcggcaa ctgcggaatg atcatctcaa cgtcagggtg atgttccgcc
    50221 agccagtttt ttaacaagct cgcttttgca gagcgcggcg agctgttgaa accgtgtaaa
    50281 taaagaagcg tagacatcag tagccttctg aagcggtatc aggttggaaa cgtgtgtccg
    50341 ccaggcgatg cacctcggtg gtcagcgtgc catcagcatg taactcgaga gtacgccagc
    50401 cgggcgcgat agtatccagc gtaaagttgg aacagtgcgg cttaaactgc acacaggtcg
    50461 acggcgttgc cagcaggcgg cgaccgttcc agtcgagatc cagctcctga tgaatatgac
    50521 cgcacagcaa gtatttgacg tgcggaaact tcgccagcac ggtatccagt tcgcccgcgt
    50581 tacgcagact gtgttgatcg agccaactac aacccgcagg tagcggatga tgatgcagca
    50641 gcagcaacgt atggcgttct ggcgcatcgg ccagtttacg ttccagccac tcaagctgaa
    50701 actcgctcag ctcaccgtgc ggcacgccaa acacctggct atccagcaac aggatttgcc
    50761 attgctcacc aataaacacg cgcttcgccg gggagatacc cgcatcctgt aacgcgctgt
    50821 acatcgcggg ctggaaatcg tggttgcccg gcagccagac gcagggcgca cgaaaacttg
    50881 cgatgccttc agcgaaatgc tgataggccg cagaggattg atcctgcgct aaatcacctg
    50941 tcgcgacaat caggtcgaat tcgtgctgat atggacgaat cgcctccagc accgcctggt
    51001 agctttccca ggtgtttacc cctaacaggg cttcgtgctt ttgtgcaaac aggtgagtat
    51061 cggtaatttg taaaatcctg actctggcct caccagccag aggaagggtt aacaggcttt
    51121 ccaaatggtg tccttaggtt tcacgacgct agtaaaccgg aatcgccatc gctccatgtg
    51181 ctaaacagta tcgcaaccag tccgctaaaa actggttaat ttgatgcttt tcgtcgcgtt
    51241 gatgcaattt tttattagga taatcatacc gcgctttgaa gcgaaaaatc tgctggcttg
    51301 aacacacttc agccaccatc gcgtcatgat acagacgcac cgtcattgac ggaaggctcc
    51361 agtaactgat cgcgggcgca gtctgttcta ttgtcaccag ggtagtgtat cgggtcgatt
    51421 ccacaatcgt cagccgatat tgtgcgtttg ccacctgata gcttacagtt tcgccgggtg
    51481 cgtcattgcg cggtaacaaa cggcgcaatt gtgaaaagtt catctcgcac aggcgcatca
    51541 tttcaggaaa gtcaggtgtg taacgcttca tttatgccca ctcatttttt aacgcttgat
    51601 gatgcagctg cagccattgc aaagcgatga ccgacgctgc gttgtcgatt ttcccctctt
    51661 ctacccactg gtatgcctgt tcccggctta ccacatgaac gcgaatatct tcgttttcat
    51721 cagccagacc atgaataccg cttgcggtcg tggcgtccac ttcgcccacc ataattgacg
    51781 aacgctcact ggtgcccccc gggcttgcca ggaaacttaa caccggtttg gtccgtttga
    51841 ctatcagtcc cgcctcttca atcgcttcgc gacgggcaac atcttccaca ctttcaccct
    51901 cttctatcat cccggcaacc atctccagta gccagggggt ttcactggtg tcgtatgcgg
    51961 caatccgaat ctgctcaatc agcacaactt catcacgcac tgggtcaaag ggtagcaaga
    52021 ctgcggcgtg accgcgctca aaaatttccc tccgtacctc atgactcatt tgcccgttga
    52081 ataaacgatg acgaaatcta taaagatcta atgaaaaaaa gccgcgataa agtgtttctc
    52141 gtgcaataat ttctacatcg tttttgccaa atgtaacggg caggttgtct ggcttaagca
    52201 ttgttaatgt cctggcacta atagtgaatt aaatgtgaat ttcagcgacg tttgactgcc
    52261 gtttgagcag tcatgtgtta aattgaggca cattaacgcc ctatggcacg taacgccaac
    52321 cttttgcggt agcggcttct gctagaatcc gcaataattt tacagtttga tcgcgctaaa
    52381 tactgcttca ccacaaggaa tgcaaatgaa gaaattgctc cccattctta tcggcctgag
    52441 cctttctggg ttcagttcgt tgagccaggc cgagaacctg atgcaagttt atcagcaagc
    52501 acgccttagt aacccggaat tgcgtaagtc tgccgccgat cgtgatgctg cctttgaaaa
    52561 aattaatgaa gcgcgcagtc cattactgcc acagctaggt ttaggtgcag attacaccta
    52621 tagcaacggc taccgcgacg cgaacggcat caactctaac gcgaccagtg cgtccctgca
    52681 gttaactcaa tccatttttg atatgtcgaa atggcgtgcg ttaacgctgc aggaaaaagc
    52741 agcagggatt caggacgtca catatcagac cgatcagcaa accttgatcc tcaacaccgc
    52801 gaccgcttat ttcaacgtgt tgaatgctat tgacgttctt tcctatacac aggcacaaaa
    52861 agaagcgatc taccgtcaat tagatcaaac cacccaacgt tttaacgtgg gcctggtagc
    52921 gataaccgac gtgcagaacg cccgcgcgca gtacgatacc gtgctggcga acgaagtgac
    52981 cgcacgtaat aaccttgata acgcggtaga gcagctgcgc cagatcaccg gtaactacta
    53041 tccagaactg gcagcgctga atgtcgaaaa ctttaaaacc gacaaaccac agccggttaa
    53101 cgcgctgctg aaagaagccg aaaaacgcaa cctgtcgctg ttacaggcac gcttgagcca
    53161 ggacctggcg cgcgagcaaa ttcgccaggc gcaggatggt cacttaccga cgctggattt
    53221 aacggcttct accgggattt ctgacacctc ttatagcggt tcgaaaactc gtggtgccgc
    53281 tggtacccag tatgacgaca gcaatatggg ccagaacaaa gtgggcctga gcttctcgct
    53341 gccgatttat cagggcggaa tggttaactc gcaggtgaaa caggcccagt acaactttgt
    53401 tggtgccagc gagcaactgg aaagcgcgca tcgtagcgtc gtgcaaaccg tacgttcctc
    53461 cttcaacaac attaatgcat ctatcagtag cattaacgcc tacaaacaag ccgtagtttc
    53521 cgctcaaagc tcattagacg cgatggaagc gggctactcg gtcggtacgc gtaccattgt
    53581 tgatgtgttg gatgcaacca ccacgctgta caacgctaag caagagctgg caaatgcgcg
    53641 ttataactac ctgattaatc agctgaatat taagtcagcc ctgggtacgt tgaacgagca
    53701 ggatctgctg gcactgaaca atgcgctgag caaaccggtt tccactaatc cggaaaacgt
    53761 tgccccgcaa acgccggaac agaatgctat tgctgatggt tatgcgcctg atagcccggc
    53821 acccgtcgtt cagcaaacat ccgcacgcac taccaccagt aacggtcata accctttccg
    53881 taactgatga cgacgacggg gcttcggccc cgtctgaacg taaggcaacg taaagatacg
    53941 ggttatctgc cgcattcttc ccccttctcg cttcaatttc gaccagccat cctctattct
    54001 gatgggtatt taccactggt cccggaagac aaaaatgaaa cggacaaaat ccatacgcca
    54061 cgcatcgttc cgcaaaaact ggagcgcgcg ccatctgaca ccagtcgctc tcgcggttgc
    54121 cactgttttt atgctggctg gctgtgaaaa gagtgatgaa acagtgtctc tctatcaaaa
    54181 tgctgacgac tgttcagctg caaacccagg caaaagcgcc gaatgtacca ccgcgtacaa
    54241 caatgcgctg aaagaagccg aacgtactgc gccgaaatac gccacccgtg aagactgtgt
    54301 tgctgaattt ggtgaaggtc agtgccagca ggcaccagcc caggctggca tggcaccaga
    54361 aaaccaggcg caggcccagc aatccagcgg gagtttctgg atgccgctga tggccggtta
    54421 catgatgggg cgtctgatgg gcggcggcgc gggatttgca cagcagccgc tgttctcctc
    54481 gaaaaaccca gccagcccgg cttacggtaa atataccgac gcgacgggta aaaactatgg
    54541 cgcagcccag ccaggccgca ctatgaccgt accgaagaca gccatggcac caaaaccggc
    54601 gaccaccact accgttaccc gtggcggttt tggtgaatct gttgccaaac aaagcactat
    54661 gcagcgtagc gcaactggca cctcttctcg ttcaatgggt ggctgatacc gatggaaaga
    54721 gtcagtatta ccgagcgccc ggactggcgt gagaaagccc acgaatacgg tttcaatttt
    54781 cacaccatgt acggcgagcc gtactggtgt gaagatgctt actacaagtt gaccctcgcc
    54841 caggttgaaa agctggaaga agtcaccgcc gaactgcacc agatgtgcct gaaagtggtg
    54901 gaaaaagtga tcgccagcga tgagctgatg accaaattcc gcattccaaa acacacctgg
    54961 agttttgtgc gtcagtcatg gctgactcac cagccatcgc tttattcgcg tcttgatctg
    55021 gcatgggatg gcactggcga acctaaactg ctggaaaata acgccgatac accaacgtca
    55081 ctatacgagg cggcgttctt tcagtggatc tggctggaag atcagcttaa cgccggtaac
    55141 ttgccggagg gcagcgacca gtttaacagt ctgcaagaaa agctaatcga tcgctttgtc
    55201 gagttgcgtg aacagtatgg cttccagttg ctgcatctca cctgctgtcg cgacacggtg
    55261 gaagatcgcg gaaccattca gtatttgcag gactgcgcga cggaagctga aatcgctacc
    55321 gagttcctct acattgacga tatcgggtta ggtgaaaaag gtcagttcac ggatttacag
    55381 gatcaggtaa tttccaacct gttcaagctg tatccatggg aatttatgtt gcgtgagatg
    55441 ttctccacca agctggagga tgcaggcgta cgctggctgg aaccggcgtg gaagagcatt
    55501 atctccaaca aagcgcttct accgctactg tgggagatgt tcccgaatca cccgaacctg
    55561 ctgcccgctt attttgcgga agatgatcat ccgcaaatgg aaaaatatgt ggttaaaccg
    55621 atcttctccc gtgaaggcgc aaacgtgtcg atcattgaga acggcaaaac cattgaagca
    55681 gcggaaggtc cgtatggcga agaagggatg attgttcagc aattccaccc gttaccgaaa
    55741 ttcggcgaca gctatatgct gattggtagc tggctggtga acgatcaacc tgccggaatt
    55801 ggcattcgtg aagaccgtgc gttgatcacc caggatatgt ctcggtttta tccacatatt
    55861 tttattgagt aatcaatgat gccggatggc attcgccatc cggtaattgt taacctatct
    55921 gcaccgacag catactcagg ctgcccattt ctataccatc gaccggaatg gtaattggct
    55981 cctgcccatc ccacgcacct aagacataca acaacggcag atagtgctcc ggcgttgggt
    56041 tcgataacgc gccaccttca tggtcgaggt aattcaccag aggatgctgc tctaccgccc
    56101 cttgccacgt cagattcgct ttcacatact cattaaacga catcgcccaa ggatatggtg
    56161 aactatcacc gtgccacttc actgtacgca ggttatgcac cacgttgcca ctggcgacca
    56221 acattattcc ttcatctcgc agcgctgcca gtttgcgccc catttcgaaa tgccaggcgg
    56281 caggtttgct actgtcgata ctcaactgca ccatcgggat atcggcgtca ggatacatct
    56341 taatcagcac gccctaggaa ccgtggtcaa agccccaggc ttctttatcc agcgtcaccg
    56401 ggaccggcgc taacagctca accagatgct gtgccagcat aggcgaaccc ggagcaggat
    56461 aatgcgtatc gtacagcgcc tgcgggaagc caccaaagtc atgaatcgtg ggcggcgtct
    56521 ccatcgcggt cactcctgtt ccacgggtaa accagtgagc cgaaaccacc acaatcgctt
    56581 gcgggcgtgg caatgtcatc cccaacttct gccagctgcg ggtatacaaa ttatcttcca
    56641 gcacgttcat cggactaccg tgacctaaaa acaatgctgg catacgtgtt gaagacatga
    56701 tgatatcctt aactaaaggt gtcattttga tatcctcaca atacgcttgt tcggcggagt
    56761 aagaacccgg ataacaatga tgatgatcat cagttatttt gacgatctgc ctgaaagtga
    56821 agatttataa ggagttgtcg atgtcagtac ctctcattct gaccatactg gcgggggcag
    56881 ccacgtttat tggcgcgttt ctcggcgttc tcgggcaaaa accctcgaac cgcttactgg
    56941 cgttttcgct aggatttgcg gcggggatca tgttgctcat ctcattaatg gaaatgcttc
    57001 ctgccgcact agcagctgaa ggaatgtcgc ctgtgttggg ttatggaatg tttatcttcg
    57061 gtctgcttgg ctattttggc ctggaccgca tgttgccaca tgcgcatccg caggatttaa
    57121 tgcaaaaatc ggtgcagccg ttgccaaaat cgatcaagcg cacagccatt ctgctcactc
    57181 tcggcatcag tctgcataac ttcccggaag ggattgccac ctttgtcacg gcgagcagca
    57241 acctggagct gggatttggc atcgcactgg ccgtcgcttt gcacaatatc cctgaaggtc
    57301 tggcagtggc aggcccggtt tatgcggcaa tggggtctaa acgcaccgcg attctgtggg
    57361 cggggatttc tggactagca gaaattcttg gtggtgtgct ggcgtggtta atcctcggta
    57421 gcatgatttc cccggtggtc atggcggcaa tcatggcggc ggttgcagga attatggtgg
    57481 cgctctcggt tgatgaatta atgccgctcg ccaaagagat tgaccctaat aataacccca
    57541 gctatggcgt actatgtgga atgtcagtga tgggattcag tttagtgctg ctacaaacgg
    57601 cgggaattgg ttaaaaacaa catcagaagt tgtccagcac atggacaact tctgccagct
    57661 tcacgaaagc cttattctac cttttcggct gtctcttctc tcgtactgtt taagtatttg
    57721 ttccgcgctt caagcagtgc gtctctgtcc tggcgaaacg cccgacaata acgtttcaaa
    57781 tgcaggaaat aacccaccac aaccagagca atcaatacaa tccagtacca ggcgataaac
    57841 atccttttac ccttaatatt taacaatact aaacatttag cgcataaatt tcacatatcc
    57901 tttttcgg
//
LOCUS       DAZGAN020000017        57006 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGAN020000017 DAZGAN020000000
VERSION     DAZGAN020000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 57006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.017"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(56..724)
                     /gene="ydcL"
                     /locus_tag="ACHKYO_RS06130"
                     /old_locus_tag="pgaptmp_001226"
     CDS             complement(56..724)
                     /gene="ydcL"
                     /locus_tag="ACHKYO_RS06130"
                     /old_locus_tag="pgaptmp_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HGU4428105.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1027..1620)
                     /gene="tehB"
                     /locus_tag="ACHKYO_RS06135"
                     /old_locus_tag="pgaptmp_001227"
     CDS             complement(1027..1620)
                     /gene="tehB"
                     /locus_tag="ACHKYO_RS06135"
                     /old_locus_tag="pgaptmp_001227"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HGU4428106.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(1617..2609)
                     /gene="tehA"
                     /locus_tag="ACHKYO_RS06140"
                     /old_locus_tag="pgaptmp_001228"
     CDS             complement(1617..2609)
                     /gene="tehA"
                     /locus_tag="ACHKYO_RS06140"
                     /old_locus_tag="pgaptmp_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HGU4428107.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            2733..3713
                     /gene="ydcK"
                     /locus_tag="ACHKYO_RS06145"
                     /old_locus_tag="pgaptmp_001229"
     CDS             2733..3713
                     /gene="ydcK"
                     /locus_tag="ACHKYO_RS06145"
                     /old_locus_tag="pgaptmp_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HGU4428108.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHALVEAFDGDTVHVRGPKVINGEGRITRTPLAGLL"
     gene            complement(3708..4244)
                     /gene="rimL"
                     /locus_tag="ACHKYO_RS06150"
                     /old_locus_tag="pgaptmp_001230"
     CDS             complement(3708..4244)
                     /gene="rimL"
                     /locus_tag="ACHKYO_RS06150"
                     /old_locus_tag="pgaptmp_001230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HGU4428109.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTVEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4307..4531)
                     /gene="ydcH"
                     /locus_tag="ACHKYO_RS06155"
                     /old_locus_tag="pgaptmp_001231"
     CDS             complement(4307..4531)
                     /gene="ydcH"
                     /locus_tag="ACHKYO_RS06155"
                     /old_locus_tag="pgaptmp_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HGU4428110.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(4671..6326)
                     /gene="opgD"
                     /locus_tag="ACHKYO_RS06160"
                     /old_locus_tag="pgaptmp_001232"
                     /gene_synonym="mdoD"
     CDS             complement(4671..6326)
                     /gene="opgD"
                     /locus_tag="ACHKYO_RS06160"
                     /old_locus_tag="pgaptmp_001232"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HGU4428111.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(6551..7894)
                     /gene="ydcJ"
                     /locus_tag="ACHKYO_RS06165"
                     /old_locus_tag="pgaptmp_001233"
     CDS             complement(6551..7894)
                     /gene="ydcJ"
                     /locus_tag="ACHKYO_RS06165"
                     /old_locus_tag="pgaptmp_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4428112.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8111..9034
                     /gene="ydcI"
                     /locus_tag="ACHKYO_RS06170"
                     /old_locus_tag="pgaptmp_001234"
     CDS             8111..9034
                     /gene="ydcI"
                     /locus_tag="ACHKYO_RS06170"
                     /old_locus_tag="pgaptmp_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4428113.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEDLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9072..10712)
                     /gene="trg"
                     /locus_tag="ACHKYO_RS06175"
                     /old_locus_tag="pgaptmp_001235"
     CDS             complement(9072..10712)
                     /gene="trg"
                     /locus_tag="ACHKYO_RS06175"
                     /old_locus_tag="pgaptmp_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HGU4428114.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            11111..11260
                     /gene="hokB"
                     /locus_tag="ACHKYO_RS06180"
                     /old_locus_tag="pgaptmp_001236"
     CDS             11111..11260
                     /gene="hokB"
                     /locus_tag="ACHKYO_RS06180"
                     /old_locus_tag="pgaptmp_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HGU4428115.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(11238..11306)
                     /gene="yncP"
                     /locus_tag="ACHKYO_RS06185"
                     /old_locus_tag="pgaptmp_001237"
     CDS             complement(11238..11306)
                     /gene="yncP"
                     /locus_tag="ACHKYO_RS06185"
                     /old_locus_tag="pgaptmp_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HGU4428116.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(11332..11505)
                     /locus_tag="ACHKYO_RS06190"
                     /old_locus_tag="pgaptmp_001238"
     CDS             complement(11332..11505)
                     /locus_tag="ACHKYO_RS06190"
                     /old_locus_tag="pgaptmp_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4428117.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(11750..12280)
                     /gene="cybB"
                     /locus_tag="ACHKYO_RS06195"
                     /old_locus_tag="pgaptmp_001239"
     CDS             complement(11750..12280)
                     /gene="cybB"
                     /locus_tag="ACHKYO_RS06195"
                     /old_locus_tag="pgaptmp_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HGU4428118.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            12469..13470
                     /gene="gap"
                     /locus_tag="ACHKYO_RS06200"
                     /old_locus_tag="pgaptmp_001240"
     CDS             12469..13470
                     /gene="gap"
                     /locus_tag="ACHKYO_RS06200"
                     /old_locus_tag="pgaptmp_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU4428119.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSISVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(13512..14951)
                     /gene="aldA"
                     /locus_tag="ACHKYO_RS06205"
                     /old_locus_tag="pgaptmp_001241"
     CDS             complement(13512..14951)
                     /gene="aldA"
                     /locus_tag="ACHKYO_RS06205"
                     /old_locus_tag="pgaptmp_001241"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGU4428120.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(15148..15948)
                     /gene="ydcF"
                     /locus_tag="ACHKYO_RS06210"
                     /old_locus_tag="pgaptmp_001242"
     CDS             complement(15148..15948)
                     /gene="ydcF"
                     /locus_tag="ACHKYO_RS06210"
                     /old_locus_tag="pgaptmp_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HGU4428121.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(16220..20122)
                     /gene="hrpA"
                     /locus_tag="ACHKYO_RS06215"
                     /old_locus_tag="pgaptmp_001243"
     CDS             complement(16220..20122)
                     /gene="hrpA"
                     /locus_tag="ACHKYO_RS06215"
                     /old_locus_tag="pgaptmp_001243"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HGU4428122.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            20323..20928
                     /gene="azoR"
                     /locus_tag="ACHKYO_RS06220"
                     /old_locus_tag="pgaptmp_001244"
     CDS             20323..20928
                     /gene="azoR"
                     /locus_tag="ACHKYO_RS06220"
                     /old_locus_tag="pgaptmp_001244"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HGU4428123.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(20979..22295)
                     /gene="ynbD"
                     /locus_tag="ACHKYO_RS06225"
                     /old_locus_tag="pgaptmp_001245"
     CDS             complement(20979..22295)
                     /gene="ynbD"
                     /locus_tag="ACHKYO_RS06225"
                     /old_locus_tag="pgaptmp_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HGU4428124.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(22285..24042)
                     /gene="ynbC"
                     /locus_tag="ACHKYO_RS06230"
                     /old_locus_tag="pgaptmp_001246"
     CDS             complement(22285..24042)
                     /gene="ynbC"
                     /locus_tag="ACHKYO_RS06230"
                     /old_locus_tag="pgaptmp_001246"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HGU4428125.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(24058..24954)
                     /gene="ynbB"
                     /locus_tag="ACHKYO_RS06235"
                     /old_locus_tag="pgaptmp_001247"
     CDS             complement(24058..24954)
                     /gene="ynbB"
                     /locus_tag="ACHKYO_RS06235"
                     /old_locus_tag="pgaptmp_001247"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4428126.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(24954..25559)
                     /gene="ynbA"
                     /locus_tag="ACHKYO_RS06240"
                     /old_locus_tag="pgaptmp_001248"
     CDS             complement(24954..25559)
                     /gene="ynbA"
                     /locus_tag="ACHKYO_RS06240"
                     /old_locus_tag="pgaptmp_001248"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4428127.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(25730..28036)
                     /gene="ydbD"
                     /locus_tag="ACHKYO_RS06245"
                     /old_locus_tag="pgaptmp_001249"
     CDS             complement(25730..28036)
                     /gene="ydbD"
                     /locus_tag="ACHKYO_RS06245"
                     /old_locus_tag="pgaptmp_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HGU4428128.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(28100..28960)
                     /gene="pdxI"
                     /locus_tag="ACHKYO_RS06250"
                     /old_locus_tag="pgaptmp_001250"
     CDS             complement(28100..28960)
                     /gene="pdxI"
                     /locus_tag="ACHKYO_RS06250"
                     /old_locus_tag="pgaptmp_001250"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HGU4428129.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVIMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(29191..29781)
                     /gene="paaY"
                     /locus_tag="ACHKYO_RS06255"
                     /old_locus_tag="pgaptmp_001251"
     CDS             complement(29191..29781)
                     /gene="paaY"
                     /locus_tag="ACHKYO_RS06255"
                     /old_locus_tag="pgaptmp_001251"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HGU4428130.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(29763..30713)
                     /gene="paaX"
                     /locus_tag="ACHKYO_RS06260"
                     /old_locus_tag="pgaptmp_001252"
     CDS             complement(29763..30713)
                     /gene="paaX"
                     /locus_tag="ACHKYO_RS06260"
                     /old_locus_tag="pgaptmp_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HGU4428131.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(30814..32127)
                     /gene="paaK"
                     /locus_tag="ACHKYO_RS06265"
                     /old_locus_tag="pgaptmp_001253"
     CDS             complement(30814..32127)
                     /gene="paaK"
                     /locus_tag="ACHKYO_RS06265"
                     /old_locus_tag="pgaptmp_001253"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HGU4428132.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(32154..33359)
                     /gene="paaJ"
                     /locus_tag="ACHKYO_RS06270"
                     /old_locus_tag="pgaptmp_001254"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(32154..33359)
                     /gene="paaJ"
                     /locus_tag="ACHKYO_RS06270"
                     /old_locus_tag="pgaptmp_001254"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HGU4428133.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(33359..33781)
                     /gene="paaI"
                     /locus_tag="ACHKYO_RS06275"
                     /old_locus_tag="pgaptmp_001255"
     CDS             complement(33359..33781)
                     /gene="paaI"
                     /locus_tag="ACHKYO_RS06275"
                     /old_locus_tag="pgaptmp_001255"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HGU4428134.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(33771..35198)
                     /gene="paaH"
                     /locus_tag="ACHKYO_RS06280"
                     /old_locus_tag="pgaptmp_001256"
     CDS             complement(33771..35198)
                     /gene="paaH"
                     /locus_tag="ACHKYO_RS06280"
                     /old_locus_tag="pgaptmp_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HGU4428135.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(35200..35988)
                     /gene="paaG"
                     /locus_tag="ACHKYO_RS06285"
                     /old_locus_tag="pgaptmp_001257"
     CDS             complement(35200..35988)
                     /gene="paaG"
                     /locus_tag="ACHKYO_RS06285"
                     /old_locus_tag="pgaptmp_001257"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HGU4428136.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(35988..36755)
                     /gene="paaF"
                     /locus_tag="ACHKYO_RS06290"
                     /old_locus_tag="pgaptmp_001258"
     CDS             complement(35988..36755)
                     /gene="paaF"
                     /locus_tag="ACHKYO_RS06290"
                     /old_locus_tag="pgaptmp_001258"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HGU4428137.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSAGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(36752..37822)
                     /gene="paaE"
                     /locus_tag="ACHKYO_RS06295"
                     /old_locus_tag="pgaptmp_001259"
     CDS             complement(36752..37822)
                     /gene="paaE"
                     /locus_tag="ACHKYO_RS06295"
                     /old_locus_tag="pgaptmp_001259"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HGU4428138.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGT
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(37830..38327)
                     /gene="paaD"
                     /locus_tag="ACHKYO_RS06300"
                     /old_locus_tag="pgaptmp_001260"
     CDS             complement(37830..38327)
                     /gene="paaD"
                     /locus_tag="ACHKYO_RS06300"
                     /old_locus_tag="pgaptmp_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HGU4428139.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(38342..39088)
                     /gene="paaC"
                     /locus_tag="ACHKYO_RS06305"
                     /old_locus_tag="pgaptmp_001261"
     CDS             complement(38342..39088)
                     /gene="paaC"
                     /locus_tag="ACHKYO_RS06305"
                     /old_locus_tag="pgaptmp_001261"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HGU4428140.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(39097..39384)
                     /gene="paaB"
                     /locus_tag="ACHKYO_RS06310"
                     /old_locus_tag="pgaptmp_001262"
     CDS             complement(39097..39384)
                     /gene="paaB"
                     /locus_tag="ACHKYO_RS06310"
                     /old_locus_tag="pgaptmp_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HGU4428141.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(39396..40325)
                     /gene="paaA"
                     /locus_tag="ACHKYO_RS06315"
                     /old_locus_tag="pgaptmp_001263"
     CDS             complement(39396..40325)
                     /gene="paaA"
                     /locus_tag="ACHKYO_RS06315"
                     /old_locus_tag="pgaptmp_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HGU4428142.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTIPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            40610..42655
                     /gene="paaZ"
                     /locus_tag="ACHKYO_RS06320"
                     /old_locus_tag="pgaptmp_001264"
     CDS             40610..42655
                     /gene="paaZ"
                     /locus_tag="ACHKYO_RS06320"
                     /old_locus_tag="pgaptmp_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HGU4428143.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFITDAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLLHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            42903..45176
                     /gene="tynA"
                     /locus_tag="ACHKYO_RS06325"
                     /old_locus_tag="pgaptmp_001265"
     CDS             42903..45176
                     /gene="tynA"
                     /locus_tag="ACHKYO_RS06325"
                     /old_locus_tag="pgaptmp_001265"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HGU4428144.1"
                     /translation="MGSPSLYSARKTALALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQSLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVDDGNYWAHPIENLVAVVDLEQKKIVKIEEGAVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(45234..46733)
                     /gene="feaB"
                     /locus_tag="ACHKYO_RS06330"
                     /old_locus_tag="pgaptmp_001266"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /pseudo
     CDS             complement(45234..46733)
                     /gene="feaB"
                     /locus_tag="ACHKYO_RS06330"
                     /old_locus_tag="pgaptmp_001266"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
     gene            46969..47874
                     /gene="feaR"
                     /locus_tag="ACHKYO_RS06335"
                     /old_locus_tag="pgaptmp_001267"
     CDS             46969..47874
                     /gene="feaR"
                     /locus_tag="ACHKYO_RS06335"
                     /old_locus_tag="pgaptmp_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HGU4428145.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(48046..48375)
                     /gene="ydbL"
                     /locus_tag="ACHKYO_RS06340"
                     /old_locus_tag="pgaptmp_001268"
     CDS             complement(48046..48375)
                     /gene="ydbL"
                     /locus_tag="ACHKYO_RS06340"
                     /old_locus_tag="pgaptmp_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HGU4428146.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(48380..48565)
                     /gene="ynbE"
                     /locus_tag="ACHKYO_RS06345"
                     /old_locus_tag="pgaptmp_001269"
     CDS             complement(48380..48565)
                     /gene="ynbE"
                     /locus_tag="ACHKYO_RS06345"
                     /old_locus_tag="pgaptmp_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HGU4428147.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(48562..51201)
                     /gene="ydbH"
                     /locus_tag="ACHKYO_RS06350"
                     /old_locus_tag="pgaptmp_001270"
     CDS             complement(48562..51201)
                     /gene="ydbH"
                     /locus_tag="ACHKYO_RS06350"
                     /old_locus_tag="pgaptmp_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HGU4428148.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            51409..52398
                     /gene="ldhA"
                     /locus_tag="ACHKYO_RS06355"
                     /old_locus_tag="pgaptmp_001271"
     CDS             51409..52398
                     /gene="ldhA"
                     /locus_tag="ACHKYO_RS06355"
                     /old_locus_tag="pgaptmp_001271"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU4428149.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            52509..52931
                     /gene="hslJ"
                     /locus_tag="ACHKYO_RS06360"
                     /old_locus_tag="pgaptmp_001272"
     CDS             52509..52931
                     /gene="hslJ"
                     /locus_tag="ACHKYO_RS06360"
                     /old_locus_tag="pgaptmp_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HGU4428150.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTNDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(52928..53194)
                     /gene="ydbJ"
                     /locus_tag="ACHKYO_RS06365"
                     /old_locus_tag="pgaptmp_001273"
     CDS             complement(52928..53194)
                     /gene="ydbJ"
                     /locus_tag="ACHKYO_RS06365"
                     /old_locus_tag="pgaptmp_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HGU4428151.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            53468..56992
                     /gene="nifJ"
                     /locus_tag="ACHKYO_RS06370"
                     /old_locus_tag="pgaptmp_001274"
     CDS             53468..56992
                     /gene="nifJ"
                     /locus_tag="ACHKYO_RS06370"
                     /old_locus_tag="pgaptmp_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HGU4428152.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT        14367 a        15011 c        13597 g        14031 t
ORIGIN      
        1 ggggttttac ggcgcaccgg ataaaaagcg ccatctaacg atggcgcgtc tggaactact
       61 ttttgttaac gtcaaacatg gtggcatcgg tcgccatgtc atcaataact tgtttaatat
      121 tttcgaaagc cattggcgta ctttcgttat tcaggtcttt accttcgccc tgacgaacga
      181 ctttgataac cggtttatta gtcgctgcat caatcagctc accttcaaaa tagaggcgag
      241 tatccatggt acggtggcct gtagccattt gcgtccccgc aaccactaat gcaacaggaa
      301 ccacttcata gaattgcagc ccttctttgc tggtatctac accggtaatg gccccacgga
      361 aaatcagact acgcggccca gcggtggtaa ccagtggttt acgctgcgct atcgcttctt
      421 tcatttcggt gttggtatag ttcaaaattt tatccagaac tttctgccct acctgggtag
      481 aaggttttgg taccggataa taagtgattg ggttccagac gatgctgtca tatttgcttt
      541 gatcaaaact cgggtctacc caacgtaaaa caggtttacc tgttgctgag gttgtttctt
      601 ttaaatcaga gtaattgttt aaaaaaccag aatatttatc tggctgggtg attttagagg
      661 cacaaccaga cagagccaac aagccgcata aagctgcaac tttcgcaaat gatgtggtac
      721 gcatgaatag tttcctgaga tgattatatt actttctaag ttatagcaaa aagcgccgct
      781 tcgtgttgtg gcaaaaaaag tgttggtgaa aataaatttt tcagcccctt ttcagacgac
      841 atagtcaatg ccgaaaaagg ggctaagtca atcatcagta gttaaatttc agtaagttaa
      901 gctgctaaaa aacttcgact actgtgatta atttcacatc ccttcgataa aaaatatgct
      961 taccgaaaag aagaacagta aatcatctca gcgtggggaa ttttcacgcg ccttattctt
     1021 agcgggtcat tttttacgtg ccagcatcgt ggcgaaacgc agtttaatac gattaccgtt
     1081 ggcgtcggtg cggtgcagct cgccgacgtc ttcattgtat ttcaccctct cccagccttc
     1141 gtaatatcga cgtaattctc cctctttgaa ggcaaacggg aagccgacgg tacatggata
     1201 atcagcggta tccatcgccg ccacaatcag gttgtaacca ccaggtttag tgcaacgttg
     1261 catattggca atcagccctg ggatggtttt agcctcaagg aacatcagta ccacagtcga
     1321 aagaataaaa tcgtactgtc tatcaaatgt gaggttattc agatcaacga ctcgggtgtg
     1381 taaattatcc agattttcaa tggatttaat gcgctcgacg ttggcgatac tcatggcatt
     1441 tttatcccat gcgtcaacat cataaccatt ggctgccagg taaagactgt tacgaccatt
     1501 gccacagccc agatccagcg ttttacccgg tttaaccact ttcaccgctt ccagtacttc
     1561 agagtgtgtg cgggttaatt catatttatc agtaaaatag ttttcgtcac gaatgatcat
     1621 tctttgtcct ctgctttcat taaaacggcg cgctcggttc tgactaacaa ttttccctgc
     1681 atcagaagcg caaaagtacg gatgagcagt attgcaataa taaaattggt aaagataaac
     1741 agcggtaccg ccagcgtatg gaaaaatcca ttttcgctgc cactccccag atgcaaaccg
     1801 gtggttgcca gtgcagatac gccgaacgag aaactccaga atgaagcatt aaatggctgg
     1861 gagagatacc atggcatcag acgtagcata aacagcagtt gcagcagtcc ataaccaaaa
     1921 agcattttcg ccagcgtatc accctcgccg ccgttgacgc tcaaccaggc actacaggcc
     1981 accagcgcag gagcgagctg aatgccgagt gatgtccgca gtgccgtggg taattctccc
     2041 gaactgcgca gacgctgcaa gatcacaggt tcaaggctta gccatgagaa aacgcctgcg
     2101 cctaaaaaca ccagaccggc gtcggtgtag cccaacgcac cacaggccat tgcgctgata
     2161 aagttgttgg caactgtcgg cagatacagt ccaggcgtgg tagcttcttc agggtgagat
     2221 ccgcgccata atcccgcagt ttgccaggcg gcataagcca actgaaccac gacaccaaaa
     2281 ctgaacaggc acaccgccag tgggcgaaac cacggaacaa aaccaatcgc caccagcatc
     2341 gtcgttgccg gaaacaaact cacaaaactg ctcagcactg gatggcgcac ttctgccagc
     2401 acgctatgcg gaaagcgtat aagtcgggta ataaatgcgc tagttaacaa tccccagatg
     2461 atcatcgcca gaatcaccag cccatcccct aaccagtggc tgacctgcca aacctggctg
     2521 gcatagcgcc aggcaaatcc catccctatc gtccccaaca caataccaaa gtagcctgcc
     2581 ggcaaattga gcactttatc gctctgcatc ttcttactca ttttgtttaa tttataaagt
     2641 atatttgaaa tgcattttat ggaattttct cgagcatagc cagagccgca gaatttgcta
     2701 cggttatagt aagtcacctg tgggaaaacg ctatgcgcaa atatcgtcta agtgaagaac
     2761 agcgagcctt tagttatcag gaagatggca ctaaaaaaaa tgtgttatta cggcagatta
     2821 tcgccattag cgattttaac gatgtgatag caggaaccgc tggcggctgg atcgatcgcg
     2881 aaacggtgtt agcacaggaa ggaaattgct ggatttacga ccagaacgct attgcgttcg
     2941 gcggagcggt gatttccggt aatacgcgca tcaccggcac cagcgtgctg tggggagagg
     3001 tttacgcaac ggataatgtc tggatcgata atagtgaaat cagccagggt gcatatataa
     3061 gtgacagcgt caccattcat gactcactgg tttgcgggca atgtagaata tttggtcacg
     3121 ccttaattaa tcaacattct atgatcgtcg cagcacaagg cttaacgcct gaccatcaac
     3181 tcctgctaca aatttatgat cgcgcaaggg ttagcgcctc gcgtattgtc caccaggcac
     3241 aaatttatgg cgatgccgtc atccggtatg cttttattga acatcgtgcc gaagtttttg
     3301 attttgccag tattgaaggt aatgaagaga acaacgtctg gctatgcgat tgcgctaaag
     3361 tctacggtca cgcgcaggtg aaagctggca tagaagaaga tgccattcca acgattcatt
     3421 acagttcgca ggtggcggaa tatgccattg tggaaggtaa ttgtgtgtta aaacatcatg
     3481 tcttgattgg cggaaatgcc gtggtacgtg gtggaccgat tctgctcgac gagcacgttg
     3541 taatacaagg tgagagccgt attacaggtg cggtgataat tgaaaaccat gtggagttaa
     3601 ccgaccacgc cctcgttgaa gcctttgatg gcgacacggt tcatgtccgt ggtccaaaag
     3661 tcattaacgg cgaggggcgc attacgcgaa cgccgctggc agggttatta tgaatcgata
     3721 atacgcgcgt ataagttcac atcatcatag gcatcattca ggaactcagc ctgtttcagg
     3781 caaccttcaa ggataaaacc attgcgcaaa gcgacctggt tgctttgcgg attgtccacc
     3841 cgacatttga tcacgaagcg tctaagttca ccagactggg cgtaatgatg aatcaatgcc
     3901 tgcagcgcct gagaaatgat cccctgcccc tgatgagatt cgtccagcca gtagcctatt
     3961 tcaacggttt tattcagtgg ttcaatacga ttaaacgaga taacgccgat aagttcatct
     4021 tctttgaaaa tcatgaacat tttggcatag ccgcgttgat gcaacatcac attaccctgc
     4081 accgtttttc gcgtgtcctc ttcactctga acgaactgcg gccagtttag cgactgctgt
     4141 aaccaggttt tatttttaca gattaactga taaagaggag tgacgtgatt ttctgcaaca
     4201 gcatgtaatt caagtgattc gcttactttt atcgtttcag tcatcttatc tccagtcaat
     4261 ccatgcagtt gttaaaaaag ccgcctggct ggcggcttag gaaagtttat acctctttga
     4321 cgctctcctg ctgcaggatt ttgagcatct catcttttaa ctgtagcttt tgttttttca
     4381 tgcggaccac ttccgcattg taccctcgac cgtcggaacc ttcctttctg gcaatttcat
     4441 gatcaagttt attgtgttta tcgaacaagg acataaagcg aggattttcg tttttcagac
     4501 gggatattaa atctcgatat tctggaaaca tacgcactcc ctgttagtgg ctgacaaggt
     4561 gtatctgaaa tacacaccat cagattactc atttaactta gggaaaaatg cgagcgggat
     4621 cgtgtttaac cagacggcac ctcccgaaga aggtgccgaa gaaaaaacga ttaactcatc
     4681 acgcggtcgt caacatactg acgtttatcc ggcgctggcg ggaaatactg atacagccat
     4741 gtttcactga tagcgtcgcc ctgacaacgc agatacatcc gcatatcgac cggatcagtg
     4801 gagtccgaag tcggatacca gtcaaactga atacgataac catcaatggg ttcaatatag
     4861 agaatttcga tttgcttcgc ttccccactg gaaagcgtaa tcaccggctc aatgcctttt
     4921 ggtgcggcag ctttcagatc accaccaacg aaatcgacgg caaaacgacg cgcccatttt
     4981 tcgggatagt gttcacctgg tgcccaacct tccgggaaac cgcccatgcc ggtacgcgtc
     5041 gccataacgc gcgctaatgg gcaatgaaca ggcggttgcg cactccagta cagacgatac
     5101 tggaatgcaa actcatcacc cgcctttaca gctttttctg gctgccagaa gcagacaatg
     5161 ttatccagcg tttcgcccgt tgttgggatt tccatcaggc cgatggtgcc cttaccccac
     5221 ttgttacgcg gttccaccca cagacttggg cgtttgttat accagcccat aatgtcctga
     5281 taatgggaga aatcacgatc cagttgcaat aaaccaaacc ctttcgggtt gttgtcggtg
     5341 taagcattga actgcaattt ttgcggatta ttcagcggac ggcaaatcca ctcgccgttg
     5401 ccccgccaca tggacagacg atcagagtca tgaatttgcg gatgaatggt gtcgcacatc
     5461 cgacgttcat tagtaccgca gctgaacata ctggtcatcg gcgcaatgcc cagctgttta
     5521 atgtctttgc gcgcatacag gtgattttcc acatccataa tcacctgact tttctcacaa
     5581 tggatagtga acttataggc accagtaatg ctggcgctat cgagcaacgc ataaacggta
     5641 aaggtagttg cccccggttt taccgtatca aaccagaagg cggtaaagtc ggggaactct
     5701 tctttactgt cggtgtaagt gtcaatcgcc aggccgcggg ccgacaaacc gtattgatat
     5761 gtgtcatcaa cggcgcggaa ataactcgct ccgaggaatg atactacatc acggcgcgcc
     5821 agttcggggg ctttaaacac gcgaaaaccg gcaaagccga gatcgctttg cccttctaat
     5881 tgtttggtat caacacctgc atcgttgtat ttgaacaact ccgggcgaaa gtgaatttca
     5941 cgcgccagat gtgttgctgg atctacagaa aacatacgaa cgcggcgacg gaatcccatt
     6001 cccatatgga agaactgagc gtccagttga cggttctcaa cgttatgcca gagcgatttt
     6061 tcggcgtcgt attgaatact gttataagcc tgcggcgtca ttgtcgccag cgtgtcaggt
     6121 aacggacgag gcgcaccacg ccacgctgtt tgcgctaagt cgtgcgccat tgactgtaga
     6181 atggagaagt caaaacgctg ggtttgcccg tcggcaatat cagaatctgc cgcgaatgcc
     6241 gcctgagaaa aaagagaagc aatgccgctg gtaccgcaca cggcggccat agccattgaa
     6301 cctttaataa atcgtctacg atccatacct gaaagtgagt ccttctggtg ttgtgaatgg
     6361 tcccgcgctg gtcgttttca gcggcaagaa agtgcttatt tatgaccgct cactctagac
     6421 aatatttatt aataatccaa ttgttgaact cagagaatat gcataaatcg gcaatttttt
     6481 tatgtcgatg ggatgagcct gattcgtttg taaaatcagg caaatttttg caaaggcgga
     6541 tggtcagaat ttaaagcaaa ccacagcgac gtttactgcg ttcttccgct tcctgataaa
     6601 gctggaactc atccaaaacc ggacaaccca acgcctgctc aaatgcctcg cgactggcat
     6661 taccgtgatt gcgtgcctgc gtttcattac ccagatttga ctggaagatc cccgccgcgc
     6721 taacgggcaa gaaatcttca taggtaatgg gttgcgccgc gacccaacca cgttcaatta
     6781 agggctgtgg atcgtctccg ggatgaatcg cctgacgatg cgcctcacct gaaggcgtca
     6841 gacggtaccg gaaccatgcc agcccttgct gacgcattaa aaactcactg tcaggaaaag
     6901 tgcggaaggt ttcctgtaaa tgcatttggt gagtgagatt atcctgcccg gttccagcgt
     6961 tacgcagaag atcatcatac agttgtcgcc ctttcggcgt taatgccacg ccacgctgct
     7021 caatttcacc aaagcgcgcg gtatgcgtgc cctgtttctg ccccgcaaac aacaccgtct
     7081 cttccagtgc tttaaagctg gtctggcgta gtaaaatcgg tacctcgcgg cgcggcggcc
     7141 cctcgatgag aattttgggt tcaattccgc attcaggcat catcgactgc acccggtcaa
     7201 tatccagcgt acgtggcgtc aggtggttga tatggcatcc aggaaaacag accacatcag
     7261 caatcaaccg atgttcgttg tgcaaggcgc gataggtttc ttcatctacc gttgctgact
     7321 ggtgccagcg aaacgtttcc agggcttcct gcacaaactc ctgtgcctgt gtttcgttaa
     7381 aaccgccctg ctgctcatat tcctctaaca gttgtcgaca acgtggggtg aagatatcgc
     7441 gctgacgcag aatctccgcc gctttctggc gcaaaatttc gttctcgata agctcaaggc
     7501 ggagtaagga ggtaaaaacg cggaagggat tacgcgccag agaagcatca tcaatgggcc
     7561 gaaatgctgt cgaatgtacc ggcacccctg cctgcgagag atcgtaatag ctcaccgggt
     7621 acatccccat aatggcaaac atccgccgaa gagtagcaag ctcttgtgca gtcccaacgc
     7681 gaatcgcccc atgacgttca acattcagtc gcgccagctc gtctgcattt accatttttt
     7741 cgtgcagttg aggattgttt tccagcacag ccagattcac atcagctacc agttccagca
     7801 gcgtgccata ttgcggaact tcttgctggt acatggctga cattgcctgc gaaaactgtt
     7861 cccgaatctc atccgccgtg atgctgttcg ccatagtgtc tgcctccagt ggtcagtaat
     7921 ctggagtgta ggtaaccgca ttcactctgg cgggaagaat ttacaaactg tgatctcgcc
     7981 gcgaaaacat caatattatc cattttgctg taacataatt gctttaattg ttaataatat
     8041 tttgcaatca agttatcata atcaaacaac ttcacttgtc agcgacaccg cttcgttttt
     8101 aacatcgctt atggaaaaaa atagtctgtt tagtcagcgc atccgtttgc gccaccttca
     8161 tacattcgta gctgtcgcac aacaaggaac tttggggcgc gcggctgaaa cccttaattt
     8221 gagtcaacct gcgctctcta agacattgaa tgaactggag cagctgactg gcgctcgctt
     8281 gtttgagcgt ggtcgtcagg gggcgcaact taccttaccc ggcgaacaat ttttaacgca
     8341 tgcagtcaga gttcttgacg ccatcaacac tgccggacag tcgcttcatc gtaaagaaga
     8401 tcttaataat gatgtcgtca gggttggtgc actacctact gcggcactgg ggatattacc
     8461 ttcggttata ggtcagtttc atcagcaaca aaaagagacg accttgcaag ttgcgacaat
     8521 gagtaaccct atgattctgg cgggtttaaa aaccggggaa atcgatatcg gcattggtcg
     8581 gatgtcagat cctgaactga tgaccgggct taattacgaa ctgctgtttc ttgaatcgct
     8641 gaagctggtt gtccgcccta atcacccgct acttcaggag aacgtaacgc taagccgggt
     8701 gctggaatgg ccggtcgttg tatcaccaga aggcactgcg ccacgccagc attcagatgc
     8761 attagtgcag agccagggct gtaaaattcc ttcgggttgt atcgaaacgc tgtctgcttc
     8821 gctatctcgt caacttacgg ttgaatacga ctacgtgtgg tttgtccctt ctggcgcggt
     8881 aaaagacgac ctgcgtcatg ccacgctggt ggccctgcct gttccgggac atggtgcagg
     8941 cgaaccgatt ggaatactga cccgcgtaga tgcgacgttc tcttctggtt gccagttgat
     9001 gattaacgct attcgaaaat caatgccgtt ctgaaaggtg aagggatctg tcgatccctc
     9061 cttgaacatt ttcacaccgt agcgaaacta actggttcac ccgctccgcg aggttctgcc
     9121 gacacagaat gtttgttcag acggaatacg tccaccgcct cagttaatcg tgccgcctgt
     9181 tcttcaaggg acaccgccgc tgctgaggcc tcttctacca gagaagcatt ctgttgcgtc
     9241 accttatcca tttcagaaat cgcctggcta acctgcgtta tgcctctact ttgttcatcc
     9301 gaggcggcgg cgatttcctg catgatatgt gtgacactcg cgacggcatc aacaatagtg
     9361 ctcatggttt tccctgccgt tgccacctca tccgacccca ggtcaattaa cctgactgat
     9421 tcactgatca agccttcaat ctctttcgcc gcttgggcgc tgcggcttgc gagtgtccgt
     9481 acttcgctgg cgacaacggc aaatccacgg ccttgctcac ccgctcgcgc ggcttcaacg
     9541 gcagcattca gtgccagaat attcgtctgg aaagcaatac tgttgatgac ggcggtgatc
     9601 tcagaaattt tcttcgaact cgtggagata gcgcccatcg tttttactac accggaaacc
     9661 gtctgcccgc catcgctggc tttaatagaa gcctcttgcg ccagtttgct ggcatgatgc
     9721 gcgttatccg cattctgttt caccgtcgca gtgagttgct ccatgctggc ggcagtttgt
     9781 tcgattgccg ccgcttgttc ttcggtgcga gatgacaggt ccgcattgcc agctgaaatt
     9841 tcgctggtgc cacgataaat ctcttccgca ccctgtcgaa cagtccctac tgtcatcccc
     9901 agtgaatgct gcatttgctg taaatgacga tttaagcgac cgatttcatt acgacccgcc
     9961 ggttcatcct tcatcgtcag atcgccacta gcgatttttt caatccgttg tgcggcatgt
    10021 tgcagtggac gaatgacgat ccgacgtagc accataaatg ttatcagcgt catgaccagg
    10081 gcaagcacaa acgcgccaat catgaacatc ccacccaggc gggtgcgctg atgggccagt
    10141 tccgccagtt ggttggccct ggtgctacgt atcttaacgg ctttgttcaa aatatcggta
    10201 taagcattat ccagcggtcg gatctgctca ctttcatgat taataatcgc ttcaaacatg
    10261 ccatttttgg catatttcaa cataggttgc atacccgtga tataagcctg aaagcgttga
    10321 tttaattcag tgtcgagggc ttcatcagca ggtgttttca ccggtcgatt ctgataagca
    10381 cgataacctt gctgcgactg tttaatctcc gattcggctt gcgcaatatt gcgcttcatt
    10441 gcttccattt ccgcaatacg actcgcagcc ccggcctgaa tcatattgat ccgggctgaa
    10501 cgcaaaaaat ctgaactgtt cgctaacccg gtccgaatct caatctccgc agtaacatca
    10561 cgctgatcgc gatcggcctg ccagaggaaa tagccagcca gagcagaact taatgcgaat
    10621 agaactaaga taccgccaag aatgcaggca aataacggaa ccagcctgat gtgatgcaaa
    10681 aaacctaatc gatgtgaggg agttgtattc attaccgttg tctctcgtcc aggtttactc
    10741 ctgatagaaa gtcatcggca cttgacggta attacttatg accaaaaacc tctttgcgtc
    10801 acatttttac aacaattatt gcagagaaaa ttaatgctat tggaatgaag agagtaacga
    10861 atggctaaac tggctaaccc gaatgacgaa aaattctgga aaacagcacg caatttcgtg
    10921 aagtgattat tttcgtccct aaggaaacct cataggatgc ctgcgggtgc ctgagacttt
    10981 ctgttttcag gctaaccggg aatcagaaag acgaaggccc cgaccgatat ttctatcaac
    11041 cgaggccaac gaatgaacct agcaattcag atcttagcct cttacccgcc atccggcaag
    11101 gagaaaggct atgaagcaca accctctggt ggtgtgtctg ctcattatct gcattacgat
    11161 tctgacattc acactcctga cccgacaaac gctctacgaa ctgcggttcc gggacggtga
    11221 taaggaggtt gctgcgctca tggcctgcac gtccaggtaa gggcaagcgc ggggattttc
    11281 cccgcgcatc tgactagcag attcattttc tcatggcacc ccttttacac ttcagttagt
    11341 gcatgttttt ttcgaggcac taatagaacc atcattacag acaaatttgc cgcctgccgt
    11401 acagtgtgag atcccccctt tcgaaccaga acagggtttg cgcccggcat cggcataaaa
    11461 ggaaacaagc gttcccataa acaaaataag tgccagtttt ttcatataac ctccatggtt
    11521 tatcgaatct tatgttcgat ttgaacgtta tacgctggtt tattttgata ccgttgatga
    11581 gatcaaagat cgggtgaatt gattaaaaat aagccagatg gacgtatggg caaggattat
    11641 ggttttattt gtcatacaaa taaatataat aggcgcttcc gatgtagacc cgtattcttc
    11701 gcctgtacca cgggtcggtt ttagtacagg cgttttcttt aaatatcctt caggaacgtt
    11761 tacgcggcat catgcgtaga agtgtgttgt ccttccagaa atagtggtgt gccagtgcag
    11821 ccgcagcgtg caacccgatg acaaaatatc ccagattcgc cagcgtctca tgccacgact
    11881 ttaagctatc aacccgttcg aaattggcct ctgaagcgta aggcatcgtc aaaccaaacg
    11941 caaaccacgg gttgccccgg ttatacatca tcaccaaacc aatcactggc agcgcaataa
    12001 acaggagata aatcaccaaa tgtcccaaat gcgccagtcc cgtcatcatc ggttttggct
    12061 taggtataat cggcggggtt gggtatttca gtcttaacaa aagacgaacc accatcagca
    12121 cgagaattga gataccacag gaaacatgaa ccatgttgat gagtggccga tcgctacgtg
    12181 ggaagaaacc acgaaactcc attgcgcaat acgctgcgat aaccagcaaa aagaccagcc
    12241 agtgaatgct gatttgtaac cttgagtatt tattttccat aacatttcct gctttaacat
    12301 aattttccgt taacataacg ggcttttctc aaaatttcat taaatattgt tcacccgttt
    12361 tcagtacgtt acgtttcact gtaagaatgg agattgccca tcccacaatc ctgggctaag
    12421 cctggaaagg atcaattttc atccgaacgt tcctgacagg agaaaaccat gagtaaagtt
    12481 ggtattaacg gttttggtcg tatcggtcga ctggtgttgc gtcgattact tgaagtcaaa
    12541 agcaacatag acgttgtcgc tattaatgat ctcacttccc caaaaattct cgcctatctg
    12601 ctgaaacatg attcaaacta tggtccgttc ccctggagcg ttgattttac ggaagattca
    12661 cttatcgttg atgggaaaag tatctcggtt tacgccgaaa aagaggctaa aaatattccg
    12721 tggaaagcga aaggggcaga aatcattgtc gaatgtactg gcttttatac ctccgccgag
    12781 aaatcgcagg cgcatcttga tgctggtgcg aagaaggtgt tgatttccgc ccctgccggt
    12841 gaaatgaaaa ctatcgttta taacgtcaat gacgacactc tggatggcaa cgacaccatt
    12901 gtttccgtgg cgtcatgcac cactaactgt cttgcgccga tggccaaagc cttgcatgac
    12961 agtttcggga tagaagtcgg cacgatgacg accattcatg cctatactgg cacccagtca
    13021 ctggtggatg gcccacgtgg taaagattta cgtgcttcac gcgcagcggc agaaaatatc
    13081 attccccaca ctacgggggc ggcaaaagcc attggtctgg tgatcccgga actgagcggc
    13141 aaactgaaag gtcatgcgca acgcgtgccg gtgaaaacag gttcggtcac tgaactggta
    13201 tcgattctcg gaaaaaaagt gactgccgaa gaggtgaata acgcacttaa acaagcaacc
    13261 accaataacg agtcatttgg ttataccgat gaagaaatag tctcttccga tatcattggc
    13321 agccatttcg gttcggtgtt tgatgccacg caaacggaaa ttaccgccgt gggcgattta
    13381 caactggtga aaacggtcgc ctggtacgat aacgaatatg gcttcgtcac gcagcttatt
    13441 cgcaccctcg aaaaattcgc taaactgtga cgcgcacagg cggaggaaaa aacctccgcc
    13501 tcttttactc attacgactg taaataaacc acctgggtct gcagatattc atgcaggcca
    13561 tgtttaccat ctgcgccgcc aataccggat ttacgccatc cggcgtggaa gccttgcata
    13621 gcttcgaagt tttcacggtt gatgtaagtt tcaccaaact tcagcccttt aatggctttc
    13681 atcgcgacgt tcagattttg ggtatagatt gatgaggtca ggccgtaatc actgtcatta
    13741 gccattgaga tagcctcttc cagcgtgtca aatgcgacca ccggcagaac cgggccaaag
    13801 gtttcctcat gcataatcga catttcctgg cgaacatcca gcagcaatgt cggcggataa
    13861 taatatcctt tcccctctac cgctttgcca cccaacgcca ctctcgcccc ttcttctact
    13921 gcgcgcgcca ctttttgctc aaccctttcc agcgccgcgg cgttaatcaa cggccccatc
    13981 gcaatgtcgt tgcgttcagc ggggttacca aattgaaccg cctgcatcgc ttcacccagc
    14041 cgattgacga actgatcata aatgcctttc tgtacataaa cacgttctgc acagttacac
    14101 acttgcccac tattaatgac gcgtgaatca acgatggctt tgactgccag ttcaagatcg
    14161 gcatcgtcca ttacgatagc tggtgcttta ccccccaatt ccagacacac tttggtgatg
    14221 tttttcgccg cagtcgccat gatcttctca cctgcagaga cgctgcctgt catactgacc
    14281 attgcgacct ttgggttacc cgccagttct tgcccaacgg tttcaccacg ccccagtaca
    14341 aggttaaaca cgccgcgcgg aaggcctatt tcatcgacga ttttggcgaa tgcaatcgca
    14401 ttgtttggcg taaattcact aggtttaatg acgatggtat taccggtcaa aagagcggga
    14461 gccattttgc gggcaatgag gaagaacggg aagttccacg gcagaatgcc ggtagtcaca
    14521 ccaagcgcac gtttaaacaa aagaatattt tctcctggac gatcgctttg aataatctcg
    14581 ccctcgtaac gccgtgccca ctccgccatg taatcgatat agtcggcagt aaaagccact
    14641 tcgacttcag ccagctgctg gatcttgccc ccttcttcaa caatcagcgc actgatttca
    14701 ctggcgcgtt cgcggatccc ggcggagatt ttgcgcaacc aactggcgcg ttcaatagca
    14761 ggcaacgctt cccattctgg ttgtgcacgt tctgctgcat cgattgcctt acgggcatcc
    14821 tcggcctgac catcgggtat gcgggaaatg acagcctctg tagcagggtt taccacatca
    14881 atccatgcgt ctccacgcca ggtaacaaac tgtccatcga tatacatagg atgttgaacg
    14941 ggtactgaca tgggcgactc ctgtgattta tatgtttgtt ttcggtgaat gcattgttaa
    15001 ttaacatcaa tcatcagaga gacggcatcg tgaaaggaca attctgtgaa gggcttcata
    15061 aaaacaagtg taaaaggaat aaaaggcaga aataaaagtc aggagtcatg cccgtttgca
    15121 aacaagtggt tcacaaacgg gcaattttta acgtaacgag cgactttcca tcgcctcgat
    15181 gagcaccgca tcatgtttca gcgtttgcca tgcatggatg acttctgccg gaaaatcaac
    15241 gtgaacgata aaatctcgcc cgcggggacc gtagccatcg ctatcatcgc gtaaacgcgg
    15301 cagctcgcca gtgagtagtg agagataacg ctcaactggc cataatcctt gtaactgatt
    15361 aataaagatt acactgtctg cgttatttcc taactgagga acgaatccgg gataacttaa
    15421 ccagcgtggt gcatcgggat tgtccccagt catacggcgg aacgtcgcca tcgtgcgccg
    15481 ctgcatggtg gggtcctgaa caacgatagc cgtatgaact cgttctacgg cctgattcaa
    15541 tagcgcgatg ctaaagcgtg cgttttcacc gcagtttgtt gactggtctt caatccagat
    15601 tttttcatgc ggaatgtgcc agaactgatg agcgatatcc gccaggatgg tcgcttctgc
    15661 tctgccagtg gtgcggatag tgttgtagtg cggatgctgt gcgatggcgc tatacaaaaa
    15721 agttgtcgag tgaccgatac caccactaat cagtaaagga atttgctgat cgcgagcaat
    15781 cttacatgcc gcgtcgatag tcggcataac cgcattgcct gcaaggatca cgcaatcggc
    15841 ctgatacggc acctcaccgg agaaatcatc ctgcgccagc cactgtccga taacatttat
    15901 ggcatctata gttgccggcg aaagcgtcgg aaacggggta atgttcatgg ttcctcctta
    15961 tcgttccctt aagaatactg cgtgagataa tagtgagaag cggagcactc tgaaaggagg
    16021 aagtcgtatt tccttaaccg ccactccgca agcctgacat atcttttaag gaaatgctgt
    16081 agataaacat tattcatctt gcagcagaga attgtatttt tctgaattcc tgaattttta
    16141 cccttaccgt tttagaatta ataaaaaacc cggcgctaaa tgcttaccgg gttttttctt
    16201 tatcaggcaa atagcagtgt taaccgctaa tctgctccat cgcctgcaaa atacgcttat
    16261 ccgaaatagg ataaggcgta ccaagttgtt gagcgaagta actaacgcgc aactcttcta
    16321 tcatccaacg gatctctttc acgtcttcat cctcacgacg tgcgggcggc agtttgttga
    16381 tccattgctg ccacgcctgc tggacgtttt cgactttcag catctgcgca cggtcgcgat
    16441 gtggatcaac cgccagtttt tccagccgtt tttcaatcgc ctgcaaatat cgcagcgtgt
    16501 cgcccagccg tttgaagccg ttaccagtga caaaaccgcg atataccaac ccgcccatct
    16561 gcgctttaat gtcagaaagc cccagcgcca tggtcatatc cacccgccct ttcagacgtt
    16621 tgttgatatt gaacactgcc gtaaggattt gctcgacctg cttcgcaata tccaccaccg
    16681 tgtcgttcag ttcggcacgc actttttcat gcagcgcagc aaagccttct tccgtccaga
    16741 ccgggccacc attggcgtcg atcaatttat ccacaccgca ggagatacag tcgtcgatca
    16801 gctccagcac tttgccatac gggttaaagt acagtcccag cttggctttg ttcggtaact
    16861 tttcatgtaa atatttgatt ggcgatggaa tattcagcag cagtagacgg cgaagaccgt
    16921 tccacattgc ctgcttttgc tccagcgggt tatcaaacag tttgatcgcc acactgtcgc
    16981 gctcatccac cagcgccggc cacgccttca ctttgtagtt gccacgcttc tgttcgtagc
    17041 tttccggcag ctgaccaaaa ctccagatat gtaagccgct ctgctcgata ccgtcatccg
    17101 ccaccgcgga cagcgtttcc tgcactttgc ctttcagcgc atctttcaga tcctgtaatg
    17161 agcgcccttc ttttagcttc ttatttttgt cgtccaccac gcggaaggtg attttcaggt
    17221 gatcgggcac ctgatcccag tgccagtctt cgcggtcaac ggtaacgccg gtcatccgcc
    17281 gtaactcgcg ctcaaggctg tcgagcaacg gtaactccag cggtttgacg cggcctaaaa
    17341 acgcttcggc atagtttggc gcgggtacaa aattacggcg taccggtttc ggcaacgatt
    17401 taatcagagc aatcaccagt tcgcggcgca gaccggggat ctgccattca aacccgcttt
    17461 cctcaacctg gttaagtaac ggcagcggaa tatgtacggt cacaccgtca gcatccgcgc
    17521 cgggctcaaa ctgatagctc aaacgcagct tgagattgcc ctgatgccag aagttcgggt
    17581 aatccagctt gctgattttt tctgcgccct ctttgatcaa catgcttttt tcaaagttga
    17641 gcaaatcagg cgtttcgcgg ctgacttttt tccaccagct atcgaagtgg cgcgcagaga
    17701 tcacatcgtg gctgatgcgc tggtcgtaga actcaaacaa cgtttcgtca tcaaccagaa
    17761 tatcgcggcg acgtgatttg tgttccagct cttccacttc ggcccgcagt ttcaggtttt
    17821 cacggaagaa tgcgtgacgc gtctgccagt caccttccac cagcgcatgg cgaataaaga
    17881 gttcacgaca taacgccgga tcgatctggc tgtagttgac cttgcgcgcg gcaacaatcg
    17941 gcaaaccata aacagtgact ttttccgttg ccatcaccgc gccctgcgcc cgttcccagt
    18001 gcggttcgct gtaggtacgt ttaatcaaat gctgagcgac aggctccacc cattccgggt
    18061 cgatacgcgc agcaatgcgc ccccacaggc ggctggtttc taccagttcc gccaccatta
    18121 cccatttcgg cggttttttg aataaaccag aaccggggaa gatggagaaa cgcgcgttac
    18181 gtgcgccggt atattcttgt ttatcggcat ctttcatgcc gatatgggaa agtaaacccg
    18241 tcagcaacgc aatgtgaatt tcgcgatact ccgccggttc gctgttaacc ggaatgccaa
    18301 gttctttcac cacctgacgc aactgggtgt agatatcctg ccattcgcgc acgcgcagat
    18361 agttgaggta atcggtacga cacaggcgac ggaaggcgtt ggaagaaagc gccttttgct
    18421 gctcgccaag ataattccac agatttacaa acgcgaggaa gtcggattct ttgtcgtgga
    18481 agcgacgatg tttttcgtcc gatgcctgct gtttgtccat cggccgctca cgcgggtcct
    18541 gaatggagag cgcggacgtg ataatcatcg cctcacgcac gcagccatgt ttctgcgctt
    18601 ccagcaccat acgcgccaga cgtgggtcga caggcaactg cgagagctgg cgaccgagcg
    18661 gcgtcagttt ataggcgctg gcctgttcat ccgtggtgat cgcgcccagt tcttcgagca
    18721 gacgcacgcc atcctggata ttgcgtttat ccggtgcttc gacaaacggg aacgcagcga
    18781 tatcgcccag ccccagcgcg gtcatctgca aaataaccga ggccaggttg gtacgcagaa
    18841 tctccggatc ggtaaactcc gggcgcgaaa ggaaatcgtc ttcggaatag agacgaatac
    18901 agatcccttc ggacacacgc ccacagcggc ctttacgctg attggcagac gcctgcgaaa
    18961 tgggttcaat cggcaaacgc tgcactttgg tgcgatagct gtagcggctg atacgtgctg
    19021 taccggggtc gataacgtat ttaatccccg gtacggtcag cgaggtttcc gcgacgttgg
    19081 tcgccagcac aatgcgccgt ccgctgtgcg actggaacac gcggttctgt tcgctgttcg
    19141 aaagccgcgc ataaagcggc aagatttcgg tatgacgcag atttagcttg ttcagcgcat
    19201 cggcggtatc gcgaatttcc cgctcaccac tcataaagat cagaatgtcg ccagggcttt
    19261 cctgactcag ttcgtctacg gcgtcaaaaa tcgcctgcaa ctgatcgcgc tcggtgtcgt
    19321 cggcttcttc aacaatcggt cgatagcgca cttccaccgg ataggtccga ccggagactt
    19381 cgataatcgg cgcattatta aagtggcgcg aaaagcgttc cgggtcgata gtcgcggaag
    19441 tgataatgat ttttaggtca ggacgccgcg gcagcaactc tttcaaatag ccgagcagaa
    19501 aatcgatatt caggctgcgt tcgtgtgctt cgtcaataat gatggtgtcg tactgcatca
    19561 gcaggcggtc ttgctggatc tccgccagca ggataccgtc ggtcatcagc ttgaccatcg
    19621 tgttatcact aacgtgatcg ctgaaacgca ctttgtaacc gatgcaaccg cccggctccg
    19681 ttttcagctc ttccgcaata cggttcgcca ccgttcttgc cgccaggcga cgcggctggg
    19741 tatggccgat cagcccttta atcccgcgcc ccagctccat acagattttc ggtaactgag
    19801 tcgttttacc agaacccgtt tccccggcga cgatcaccac ctggtgatca cgaatcgctt
    19861 cgagaatgtc ctgttttttc tgactaacag gtaaattgtc aggataagta atttccggtc
    19921 gtgccgcttc acgcagcagg actttccctg ccgcttggtc aatctctttc gccatctcct
    19981 ggaaaatggc ctgttgtgca tcaggatttt taaccttctt aacaccgtgc agacgccgag
    20041 aaaaacgcag tctgtcgcgc agcatcagcg aatccagccg ctgctgcaag gccgtaaagg
    20101 tcaatttttg ttgttctgtc ataacgttag tgggcagtag tctgcctgat taattttctt
    20161 ttaaatgata gatatagagt accacatcgc ggctttctat gcgttgttca ataaattcga
    20221 acataggctt cgatttattg cgctatctct gtggcaggat tgtgaataaa gtgtcagcaa
    20281 gcaacggggc atcgcccatt caaacatcta taaggaaaca ccatgagcaa ggtattagtt
    20341 cttaaatcca gcatcctggc agggtactct cagtctaatc agttgtccga ttattttgtt
    20401 gaacaatggc gcgaaaagca ctccgctgat gaaatcaccg ttcgcgacct ggctgcaaat
    20461 ccgattccgg tactggatgg cgaactggtt ggtgcactgc gtccgagcga tgcgccgctg
    20521 actccgcgtc agcaggaagc tctggcgctt tccgatgagc tgattgccga gctgaaagcc
    20581 cacgacgtta tcgttattgc ggcaccgatg tataacttca acatctcaac tcagttgaaa
    20641 aactattttg acctggttgc acgcgcaggc gttactttcc gctataccga gaacggtccg
    20701 gaaggtctgg taacgggtaa aaaagccatc gttattacca gccgcggtgg gatccacaaa
    20761 gatggaccaa cggacctggt gacgccgtat ctgtccacgt tcctcggctt tatcggcatt
    20821 accgatgtga aatttgtctt cgccgaaggg atcgcatacg gtccggaaat ggcagcgaaa
    20881 gcgcagtctg acgcgaaagc ggccatcgac agcattgttg ctgcataacc ggacacgatc
    20941 ttgatgtgga ctgtagttca gtccacatct caatccactt acctgttttc ccataatttc
    21001 agcatcgctt tgtgatcttc cttaagcaca atatgcgaac gtctggctcg aatataacta
    21061 atcgcttcat caacggtttt acagtgtccg taacataaca accatgccgc cactaccagc
    21121 gcactgcgcg ataatcccaa cgcgcaatgg accagaacgc tgccttgctc ttcgcgtaat
    21181 gtttccagca tcgccacggc ctgtcggagc tccccctctt ccggaaccac cagatccagc
    21241 atcggtacac agaaatagag tcgatctttt gtcgctcgtc cccgagggaa ttcaaaggtg
    21301 acgtccagaa ccgcattctg tgccggaata tgtcgtggaa acgcccctaa ataaacacca
    21361 gcagtaattt tgctcaccgg ctccaggcga cgacaaaacc aacgcataga cagccacatc
    21421 ccgatgcgcc agggcaatgt cagccagtaa acggcgggcg ggagtttccc ctgactatct
    21481 ttccctgttg ttatcgcgcc aagcccaccg tacccacggc caataatgag tagcgataat
    21541 actggccaac ataaccagac tgaccaccat aactgaatca tcaccattag ctccatcaac
    21601 acaatgcacg agcccgcgcc tacgacatat ggcagtgcta ttttgattcg acgttgatca
    21661 ggtttctgat aattccaacg acgatcgacg ggcaccatcc agtcaatcaa catacctacc
    21721 gccagccctg tgatgacatc aataaaatga tgctgccagg tcgtcagagt agaaatagcg
    21781 atgagtaaaa accatccgcc acagacttta cgccacctct cagccagatg ctgacgaaag
    21841 tgacgccaaa gtagccagca gagaataata tgcagcgaag gagactggtt ataaggcaga
    21901 tcaaacagtt caagttgcga aaatagccat cccgtcaccc cactcacttc aggacggata
    21961 aaactaaact tcagcggata gagcaaaaaa ccgcagcagg ccattaccgt tgccagaata
    22021 agccggtgga caagtcggcg ctgttcgaat gtcgtgctac aaacgaacag cgaaaatcca
    22081 tataaaagat ccagactcca gtaaggaaca atagtccagg gaagaaaagg gatcgccgtt
    22141 tcccaaccga atacctgact ggggatatca tggctgttaa ggtcctgaac cgcggtgaac
    22201 tgattaagag atccataggt gaagaagaaa aacggggcca gcaacaataa ccagccagcg
    22261 ccttgtagca atacgttccg acgttcagtt atcacgacgc accgccatcg aaaccgtaaa
    22321 aataccccac tcatcaatcc gttgtgtgca tttatcaaat ccggcatcac gcacgagtga
    22381 atccatctcc ccttgcgaac gcacgcgcat tacccacggt ttaccatctt tatgactggt
    22441 taacaccccg gcaatcatct ccagttgagg gtgccacggc tgcccggtgt agatgagaat
    22501 gccgcccggt tcgatggcat tggcaagacc tgcgagtgag ttttttacct gctcattttc
    22561 gggaaaaagc tcatacaggc cagagacaat cgccagcgta ggccgcggag ttaacgcgct
    22621 gagttcctcc gggttaaacg catcgccctg ttcaaaacgc acccgcccag acattccccg
    22681 ttgggcaatc atcgcttgcc cctgtgcaac atttaactca ctgtaatcac gtaacaaaat
    22741 atcgcttacg gcaggctcgt ttgccagcgc atccagtaca tagcgcccat gccctgcggc
    22801 aatgtcaacc acgcggacgg ctaaaccttt ggcgtggaga tcggcaacgg cttgtttaat
    22861 cagtatttgt aaatgggttt tacgctggcg aataccgcgc cagccaacac tgttcaggta
    22921 gattttgtca attaagcgcc cgaatgcgtt actgccttgc ggttgatttt gatagacata
    22981 atccagggaa ctgcctgaat caaagccggt gctcattccg agatgcaggc ccgcagagtg
    23041 cgccccgaac aatttcatcg cctttcgcat aaagcgatac gccaaatcaa ccggcgataa
    23101 tggcacgggt ccacctgaaa ggagccgcca gcgatccgct gatggtccag tgcggtcttc
    23161 atgctgataa tcaaattttt gtgacttgtt agcatataaa cgactaataa agctttgcat
    23221 tttttcaaat gcctgcgccc tgttctcttc acccaacgtg tcgtgataaa agcctggcag
    23281 cagatgcagc tctttcagag ggctacgtaa tctctgataa aaatcaatct gcggttggcg
    23341 atgcaccaca tagtcatcgc ctgatatcag aagttgcgtg gggagcgtaa tcgccgccgc
    23401 atctctaata atacgttcag acgttttgta gagatcgagc aagatattaa cggcaatcgc
    23461 ccgtgtgatc agcggatcat tattgaaact cgccacccgt tgccgatcgt gggtcaaata
    23521 gcgtcctttc acataggaat taataaaaaa cagaccacgc agacgatgcc ataacgccag
    23581 cgcaggacgt gccagcggta catacaattt aaccttaaag gccggagaag ccagcaccag
    23641 cccgcgaatt gcaggcgcat aatcatgaac ccatgtggca accagcactg cgccgacgct
    23701 ttgcgcgatc actaccacct catccagtcc gacctggcta tcgctggcag caaaacgaac
    23761 aaattcatcg acatcctgca ctgaacgcgc aagagatggg ctataaccac gcggccccga
    23821 agtttgtcca tgccctcggg catcccaggc ataaaaaaca gtatctggca tcgccagttc
    23881 atcaacgaga tgttgtagac gaccagaatg ttcatgaccg cgatgaaata agacgatgac
    23941 ctttttagcc ccgggctgta aagcgggcca gtgccgataa aacaacgctg tattatcact
    24001 gctggtaaaa aaatgttccc catggatgcg tgaattttcc attttctcta ttttccttca
    24061 gtaacagcag tagcgaataa aataaaaaaa taccggagcg gtgaaaatta atgagtcaat
    24121 cctgtcaagg agtccgccat gtcctggtaa tagtttccca ctatctttaa caccaatatc
    24181 tcgtttgatg gctgacatca cgacatcgcc gcaaaaacca ctaataccaa ttaacaaacc
    24241 cgctaataat gcctgtaatg tatttagcgg tgtcagcaac ggtccaataa tcagtgacgc
    24301 aatcatggtg gtgattacgc cccccaccag accttccagc gtttttcccg ggctgacttt
    24361 ggggaccact tttcttctgc cgcaggattt cccccataaa tactgtgcaa tatcgtttga
    24421 ctcggttaat gccaaaagaa ataacaccag taacgccccg ccctggatat ttgtggttgg
    24481 caacattaat aaccaggcgg catgactcaa ggcgaaaacg gtcattatcc agccccaaaa
    24541 aatggcgctc acggtatgca gaaaaccgga ggggtccccc ataaaaactt gcccggtggc
    24601 taatattaga aacccagcca aaggtataaa taatagatac agctcaaagc aattaaaacc
    24661 gataaacaaa tagttaagag gaataaccca atataaccac cgcggaaaat gtacagatat
    24721 aagcgtacaa tattctttca atgccagaaa acttatcagc gcaaaaaacg tcaacgtcat
    24781 ccagcgtgga cctgaaatca ccagtgaaaa acaaatgacg atgccccacc aggtacgaat
    24841 acgtaatgta acttcatcct cttttctctc cggtaaccgc catagcagaa aatggtttat
    24901 cagcgttgct aaaattaaca acgcgaaaag tgttgccaga gatttttcca gcattatctt
    24961 tcagcgctca taagaagcac actccgacaa cgattaatcg cagtccagag aagcagaatt
    25021 gaagcaatgc tccataaaag attattccac tgcatccact gtggataaat ggcaacggcc
    25081 agaccccaca gaccaaatat taacgcgcga tcgcttttgc caaatggacc ggcataactg
    25141 cgaacaccgt taatcgtctg cgcgagtaaa ccgcaaaact cggtcaatat ggtgcaaaat
    25201 aacatgagta tcacgagtga tgcgttactt tccggtaaaa ataaaaatgg taaatagagc
    25261 gcaatatcgg aaataacatc accagtctca ttcaaaattg ccccaagccg tgtttgctga
    25321 ttgcactcac gcgccaacat gccatccagc gcattgagcg ccatacggat aaaaagcacg
    25381 atgggcaata gcataaagag gatgggttgt gccaccaaca tcaacagcaa tccggtaaga
    25441 agagaaagcg ccagtgcagc aagagtgatg tgattcgctg taacgtggtg cttgtaaagc
    25501 caaaacatcg tcggccttaa cagcgactga aagagcggtt ttatctggta gagcgtcatt
    25561 atcaatcctt gataattcgg tttgcgctat ggtgcatcaa ttcccctgac ggagatatcg
    25621 caacacgccg cttcattaaa aattcagaat atatcaaggt attggggact cactgtcagg
    25681 aataattata cccaattaat caaagggtta agaaatatta cgttgtggat caatacaact
    25741 catcaagttt tctcatcgct tgttcccgta ttgcgcgcgt cttaccgtta agcgccagct
    25801 ggcggactaa atccagttca gggttaacaa gctctaacgc cagcaaagga tcacgtttca
    25861 gcctcgcctt taatacatca actggaaaac ggggattatt catcgccaca atccaccact
    25921 cgggatcaat ttctgccgcc agtactgcag gcggtgtgtg gcggttgtta agtgcgccga
    25981 acttgaaaaa tgtgagattg cttttcgcca gtgtactcca ccatatacga tccggttgtt
    26041 ttgccagtac atccagttgt gctaacgcat cggggtcgag tgcataatag acgcgtaatg
    26101 cttcatcaac ctgcgcacgg gagctgtttt cctgcctcag aaaaaacttc gcccaaatat
    26161 cattgcgtgg cgtattttgc tgtaattcct tgaggacaac agcgggtaaa ttatcccaac
    26221 taactaatgc actctctaaa aagtcccagt cttccaaacg tatcagggcg gtggcattta
    26281 atagcatctg atcatcaact tcaccgtggc tgataagctc agacaaaatc atatcagcgc
    26341 tggtttcata cgcagctgaa tctgctttcg gattattttt ccgatgcgat aaagcacgtt
    26401 gccaaatttt ttgtttcatc gacgtgctca gatactgatt tttcgccaga ttactccacc
    26461 cctcaggatc gttgaaacgt gtcaataacc gttgtagagc agcgtcagag tgaattacct
    26521 gcatatcccg acagcaaaag taggcagggg attttttctc catcctttta acaaaattaa
    26581 actgtaagtt atctggcaat gctaaaaata acacacgtat tagatcatcg tcattctcga
    26641 atttttggta taaataggct gcaactttgt cactctcttg tggcgtcatc cgcaataatt
    26701 gaccatggtg tttcatctct gccagcttga acgctaagct tgtgacaaca ttgtttgatg
    26761 aatcgttcgc caactgcatt gcctgattgt aggtgagttg tcctccagca ccgacctgac
    26821 gaatagagga atcatcactg cggatatagc catcaataat ggattcaggc aataaccacg
    26881 catctttata aagctcctga atgatcatat cccaatctct tttcgccaga gtcattacga
    26941 tatccagagg gagattttca cgtagcgcaa gattcaacgg accgacacca tcaggtagac
    27001 gactcattcg cgtcattatt tcgacagaga gcggttcacg gtcgagcaac cccagaacaa
    27061 caggctggta acccaattgc tgatcggtca actccagaat ctcattgtca ggcatatatc
    27121 tcgccatact ttcaatcgtt tgttcatcat tgctattaat aagttgtttg cgtaggttag
    27181 ctgaaattcc tttaagcctt gggagagttc gcattatggc aagatcaaat gtcgcaatat
    27241 ctcgtccttc aaatgcagca acgttgcctt catccagcat ttgcaattcc tgcactaact
    27301 caggagaagg cttagggcga aacagcgctt cttttttcat ctgattacag acattccctg
    27361 ccatgcgaat attgcttcgc ttaaaaattt ctttcagagc atcttcatca tttattagac
    27421 cccaataata tgcatcagga tttattatca tcagcgcttt tttgacattt tcagtgtcat
    27481 ttttgtcact cgcacggacc atgaattcac tatttgtcga agacgcaaca acaggtgcaa
    27541 ttatgtcatt gataaatatt ccacgcgcat taatactccc cttaatcgaa gacggattat
    27601 cgtaatatga gtcgataaat accagtccac ttgcatttat cgaaccggta attaacagtg
    27661 gtgaatccgt aactatattc gttgccgtca cgttaccgag aataacccat ggattagaaa
    27721 gagagaaagt ggaaatatta cctttgactg tcaaattacc aagaacaatc aatttttgat
    27781 tactctctat attaccttct accactaaat cgccacgatg cactttccag tccgatgcag
    27841 ataaaccgaa ggggacaaga ttcacgctac tgttgatagc ttgttcaact tgttgtttat
    27901 cttcagcggt agccgcagaa aaaaatggtt caatatcaac tgtatttgca acacatcgaa
    27961 gttccgcctg tactggaaga ctcagcataa acaggggaag taaacctatc cagccgcaaa
    28021 tcctggcctt aaacatatgc actccatgcc attaaaaaag aaacaaaagg gaaatgcatc
    28081 tttccctttt gtatattcgt tattctcgcg aaataccatc caacgtagac aacacttcct
    28141 cagaaagatg caatttttca gcagccatat tctcccgtaa atgcgcaaca gaagacgtcc
    28201 ctgggatcag caaaatattc ggtgaacgtt gtaacagcca cgccagcgcc acctgcatgg
    28261 gtgttgcacc caggctcgca gcaacatccg acagcgtaga ggattgcagc ggtgtaaagc
    28321 ccccgagcgg gaagaacggc acgtaggcaa tgccgtcacg agccaaagca tcaatcattg
    28381 cgtcatcagc acgatgagca atgttgtatt cgttctgcac acagacgatt tcggcaatct
    28441 tgcgcgcctc tgcaacctgc gtcggtgtga cgttgctcag gccaatatgt tttaccaggc
    28501 cttgttgttg catctctgcc agcacggtca ggctggcttc aatcgatcct tccgctgggc
    28561 catgaccatc ccccataata acgcgcaggt taaccacatc cagtacgtcc aggccgagat
    28621 tacgtagatt atcgtgcacc gctttttgca actctgccgg agaaaaggcg ggcaaccagg
    28681 aagcgtcctc tccacgccgc gcaccaattt tagtgacaat tgtcaggtcg tcagagtaag
    28741 gataaagcgc ttcgcggata atctgattgg tgacgtgcgg accataaaag tcgctggtat
    28801 caatgtgatt gacgcccaat gccagcgcct cgcgcagcac ggttatagcg acgtggcgat
    28861 ctcttggggg gccaaaaacc ccaggacctg ccagttgcat cgcgccataa ccaagacggt
    28921 taacggattt tgtaccgaga gtaaatgtat tgctgctcat cgcaccctgc cctgtaataa
    28981 aacggattaa gaaaaatcag tatagaaacg aaaagagata aataatgcga gggcgtaaac
    29041 aagattactg gatgagttgc tctattgtcc tgaaaaacac aaaacaaaac gcgtaaagcg
    29101 ccgcatccgg caataaaaaa accgcaacct gtatcagcgt tgcggtttag gtatcacatg
    29161 ctaaaaatta gaatgttcag tatctggacg ttactgtttc ggtcgcagat tctcatcaaa
    29221 taccagacgt ttcctgttcg gttcaacttc ccgcaatggc tcaacttgat gcaacgtctg
    29281 cttacaacga gtcaccagca cctgatactc atgcgtaccc tgctttttcc atgccagctc
    29341 ctgctcactg agttcacgaa tcgctttcgc cgggctgccg acaatcaggt aattggcagg
    29401 catttctgct ttggctttca caaatgcgga tgcgccaaca atgctgttct cgccaatcac
    29461 cgcgccgtcc atcactaccg cgttcattcc cactaatgca ttgcggcgga taatgcaacc
    29521 atgaaggata gcgctatgac caatatgtcc atcttcttcc acgacagtat cctgctcggg
    29581 aaaaccgtgc ataacgcaat tatcctgaat gttcgcgcca tctttcacca cgatacgacc
    29641 aaaatcgcca cgcaggctgg catttggccc aacgtaaacg cccttgccta gaataacatc
    29701 gccgatcaat accgctgtcg gatggacaaa actctcttct ggcacaaccg gagtcagacc
    29761 atctatctgg taaattggca tatcgcctcc tgttcaatat tcaaaccgcc aaaacgttga
    29821 aaatacaggc ttcccggcgc aggcagttca ccgaccgagg tttcaccttt ttcactaacg
    29881 aacgctaaag cagcaggcgc tacgcgctga taaatgttga tacacagctg acgcgccgta
    29941 tgccctgccc agtgcgccgg aagcaactcc tctggcaaca atgggtcttt aaggacgaca
    30001 cggcgataaa aatggatcag taaaagctga atatgaaatg cacgctccgg ggttaactcg
    30061 tctgctgcct cttttaacag cggcaccagc gggcggaatg actgaataaa ggtttcgtac
    30121 atggcatttt gttcagttaa atgccagcac tcttccactc tggcacgcag tgctgcacga
    30181 gaaagcgcca gtggtatttg cgcttcaaaa caaatcacgt tatccgccac ccccgcttca
    30241 tgcaaaagtg tctgtacatc cgccagtttt tgcgacggcg atgccatcag gctgggtgcc
    30301 agtgcgccaa aaccttgcca gatcaactgc tttttgacat cagccagcgt tgatttatct
    30361 aaaccttccg agagcaacag gagccattta ccatcccatg caggttgctc tgcgcgataa
    30421 attttacttt ctgcccgtcg cgtcaggcgc aagcctttat cactgaggct atagaaactg
    30481 cgtcgcccga tgcgggaaac atccagccag ccttctttat taagacgaaa caaagcggtg
    30541 cgcacgaaac gctcaccaaa tcccagccct tccagcaaag cagccagact acccagccag
    30601 atttcaccac cacgatggga aagcgaatca ccatacagag aggagatcaa agatgtgcca
    30661 ctgaccggaa cagcgtttac agcatgttgg ataaaagtaa caagtttact cattcgattc
    30721 agtctgttgt tattgttgtg atttatgaat catagcacag gtcgtatcga cttctggccc
    30781 tggcgcttta cctcaccagg gccagacgac acgtcaggca ccaacaatat tgcgcagatc
    30841 aaacacccga cacgccttgc cttctgaacg cgggatactg ccacagttaa cgatcatcac
    30901 atcggtagag atccccacca tcgacttaat ccgatggcgc agctggtggc acacctggca
    30961 acgctgctca tgcgtcaatg tcaggctgct ttctttcaac tccactttca cagaaagtga
    31021 atcaagatgc ccccggcggt tcacctccag ttggtaatgg ggcgataaat gttcgaattt
    31081 gacaatctct tcttccagtt gtgacgggaa gacatttaca ccgcgaatga tcaacatgtc
    31141 atcgctgcgt ccgctgatgc gatccatgcg gcgcatagtc cgcgccgttc ctggtaacag
    31201 tcgggttaaa tcacgcgtgc ggtaacgaat gaccggcaat gcttctttgg tcagcgtggt
    31261 gaataacagt tcgccatgtt caccatcggc aagcggtgtg ccgtcatgag gattaacaat
    31321 ttcaggatag aaatgatctt cccaaatggt tgggccgtcg gtagtttcca gacactccat
    31381 cgccaccccc ggccccatca cttctgacag gccataaata tccagtgcgg tgatccccag
    31441 acgccgttca atctctttac gcatggcctg tgtccacggc tcagcaccaa acactccaac
    31501 ccgcagcgag caaccgctgg catcaccgcc caactgccgc tccagctctt caatcaggtt
    31561 aaggcaataa gatggcgtaa ccatgatcat atctggttga aaatcgcgga tcagttgcgc
    31621 ttgtttttcc gtctgcccac cagacatcgg gatcaccgta gcgcccagac gttcggcacc
    31681 atagtgcgcg cccagcccac cagtaaacag gccgtaacca taggcaacat gaattttgtc
    31741 tttgggcgtg ccacctgccg cacgcaagga acgcgctaca atattggccc agttatcaat
    31801 atcgttttgc gtatagccga caactgtcgg tttacctgtg gttcccgaag aagcatgaat
    31861 gcgcaccact tgttccatcg gcacggcaaa ggtgtcgaag ggatagttgt cgcgcagatc
    31921 ctgtttggtg gtacagggaa atttacgcag atctgaaagt tccctgaaat catcaggatg
    31981 tacgcctgct gcgtcaaatt tgcgccgata catcgggaca ttttcatacg cgtgtttgag
    32041 cgtccatttc agacgttgtg tttgcaacgc ctgtaactca tcaacagacg cggtttcaat
    32101 cgggtcaagc tttgtattgg ttatcattgt agggtactcg caggttgata tgctcaaaca
    32161 cgctccagaa tcatggcgat gccctgaccg acaccgatgc acatggtgca caatgcgtaa
    32221 cgaccgttac gccgatgcag ctcatggctg gcagccagtg ccaggcgggc accactcatt
    32281 cccaacggat ggcctaaggc gatagcgcct ccgttggggt taacatgtgg ggcatcatca
    32341 ggcagcccca attcgcgtag tacacccaac gcctgggccg cgaacgcttc gttcagttca
    32401 atcacgtcca tatcgtgaat actcagcccc gcgcgttcca gcacccggcg agttgcaggc
    32461 accggaccaa gccccatcag gcgcggttcc accccggcgg ttgccatggc tacgattcgc
    32521 gcccgcggtg tcagtccttg cgctgctgcc atctgttcac tggcaataat caacgcagcg
    32581 gctccgtcat tcaccccgga ggcattgcct gcggtaatca ccccattggc acgaaatggt
    32641 gcttttaacc cacgtaactg ttccagcgtc gtttccgggc gcagatgctc atcatgttgt
    32701 atttctgtta caacgccttt cttgtttttc aacacaaccg gaacaatctc ctcagccaga
    32761 atgcctgagg attgcgcttt tgccgtacgt tgctgactgc gtagcgcaaa actatcttga
    32821 tcttctcgcg atatttttaa cagttcagct acattctctg ccgtttccgg catgctgtca
    32881 gttccaaatt gttgagccat gagcgggttc acaaatcgcc agccaatagt ggtatcaaac
    32941 atctcagcct gacgagaaaa tgcactggtt gccttgccca taacaaacgg tgcccgagac
    33001 attgactcca cgccaccagc gatcagcaaa tcgccatcgc ccgctttaat cgcccgtgcg
    33061 gcgaacccca gtgcgtccag cccggaacca cacaagcggt taatggttgt gccggaaaca
    33121 ctctgcggca gccccgccag taacgtcgcc atccgggcta cgttacggtt gtcttctccc
    33181 gcctgattag cacagccgag gatcacatca tcgatacact ccgcatcgag acgcgggttt
    33241 cgcaccagca gctcccgcaa agggatagca gccagatcgt cagcccgaac acctgataat
    33301 gccccaccat agcggccaat tggcgtacga attccgtcac agataaaggc ttcacgcatc
    33361 aggcttctcc tgtaatggtg ccgccgatgc ggtgagattt accgcgaaac agcgcaaccg
    33421 ttttttgttg ttggttaaca atttcgatgt cgtaaacacc ggtttgcttg ccctggtgac
    33481 gtacctgcgc agtagcagtt aaggtgtctc cggcaaagcc tggacgcaaa aaatcaatcg
    33541 tgcaggcaga agcgacggct gccagcccct ggctattgca ggcgtaggca aaggcagtat
    33601 cagccagtga aaatagctgc ccgccgtggc aactttgatg accgttaagc atttgtgcag
    33661 tgacggtcat ggtcactaca gcaaagcctt catccattga gataatgtcg atgccaagcg
    33721 ctttggcgca ggcatcgttc tcatacattg catgggcatt ttgccaggcc ttatgactca
    33781 taaccgctct ccagaagcgc tcgttggcgc agcaatgaac atgggcgata gcgttcttcg
    33841 ccatagtgat gttgtagatt ttccaggagc cttaatattc gctgccagcc aagttgcgct
    33901 ccccaggcaa gcgggccata tggataattc accccaagac gcatggcggt atcgatatcc
    33961 tgttcagagg ccacgccttt ttgcagcgca tcaagggctt cattgatgat cattgctacc
    34021 gttcgccaaa tcagcattcc tgggtaatct gcaatttgca gcactgtttt gccctgctgt
    34081 tgcaggtaat aaatggcctt gcgggtcgct gagtccgggt tcaccgctgc cgcagcaatg
    34141 gtcaccacct tgcccgccat tttgtcgacc actaccaccg ggcgtgccag tcgtattgcc
    34201 agcgcctgtg ccgtctcgcc ttgtgtctca atcaataaaa catcgtcaat ttccgtgacg
    34261 ccgtcacttt tcttttctac tttcattggg ctaaaactgt cgcttaccgc ttccaggcca
    34321 acaactgcct cacgttccgc gcgccagtcg tacacgccca gcccactttt cttgcccaac
    34381 cgtccaccaa tcaccagttc ctgttgcacc agcgaaggta aaaaacgacg ctcctgccag
    34441 aaagcgttaa acaccgaaca ggtgacagca aaattgacgt cctgaccaat cagatcggtt
    34501 aattccagcg gccccatcgg gaaaccagcg ccatcgcgaa gtgcagcgtc aatcacttct
    34561 ggtgcagcaa cctgctcttc cagtgcccgc caggcctcgg aataataagg acgcgcaaca
    34621 cggttaacga taaatccggg ggtcgaatga cagcgcactg gctgcttacc ccaactcaat
    34681 gtcagttcac acaactgctc gacaacttcc gctgccgttg ccagcccact gaccacctcc
    34741 accaacttca tcaccggtgc cgggttaaaa aaatgcagcc ccgcaacacg ttcaggattt
    34801 tttacctccg cagcaatcgc ggttatagag attgacgaag tgttagtggt caatagcgtt
    34861 tgtggcgggc aaacttccgc cagttgtgca aagagcgctt ttttaacttc cagacgttca
    34921 gacgccgctt caataaccag attcgctgct gccagcgcgt gaatatcggt caccgggatc
    34981 aggcgtttca atgtgcgttc acaggtttca gcggtcagtt ttccccgcgt cacgcgtgaa
    35041 tttagccgcg cgtgtatccc gtcgattgcg cgggtcagcg cttcagcaga aatgtcatac
    35101 agtaaaacct gatgtccatg actggcggca acttcagcaa tgcctgcccc catggtgccg
    35161 ctcccaatca ctgccacagt ttgcacattt atcatcatgc tatttccccg tgaactgcgg
    35221 tgagcgttta gccaggaacg cactgacacc ttcacgataa tctgcgctcc gtccggcaag
    35281 tcgctgatag tcacgttcca gatccagttg cgtatcgagc gtattggttt cagcgctatt
    35341 tatcgcttgc ttgataagtc caagaccaaa tgtcggttgt gtcgccagat gccgtgccag
    35401 ctgttgcgcg gtatctgcca gcgtttcatc atcaacaacc tgccagatca tcccccattc
    35461 gtgcgcctgt tcagcactca gttgattccc cagcagtgcc agccccatgg cgcgcgctcg
    35521 cccagcaacg cgtggcagta accaggttcc accgcaatcg ggtactaaac ctaacttact
    35581 aaacgccatg acgaattttg ctgaacgggc agcaataacg atgtcgcatc ccagtgccag
    35641 tgttgcgcca gcccctgccg ccacgccatt gactgcacag atcaccggtt ttggcagttt
    35701 tgccaggcga cgtaccagtg ggttatagaa acgttcaact gacatcccta aatccggtgc
    35761 ggggcctgtg ggatcaacgt tacgatcgtt aagatcctga ccagcacaaa atccgcgccc
    35821 ggcacctgta agtaacaggc aacggatagt gtcgtcgcgc tcgacctgtt tcaggcactc
    35881 tgccagttgt gcgtgcatct catcattaaa actgttcagg cgttccgggc ggttgagcgt
    35941 tagtgtcatc acgccctttt ctacatgact gaggatgaat tccatcatta gcgtccttta
    36001 aagtcgggcg tgcgtttttg caagaaagcg gagatgcctt catgacgatc ttctgttgcc
    36061 gccagcaagg tgaataactg tcgctcctgg gcaagtccgg cttgcaaagc cacttcctgc
    36121 gactggcgca gcgcttgctt tgccgcttgt aaggccagcg gcgagtgacg tgccattttc
    36181 gacgccagct gtaaggcgta ttcgagggtt aaatcgctgg ggaagacgtc gctaaccagc
    36241 ccggcctgct gtgcttgctg agcggtgata ctttctccgc tcagcaccat tttgctggct
    36301 aacgatttac cggcactacg gattaaacgt tgcgttcctc ctgcgcctgg catgatgccg
    36361 agagtgattt ccggcaaacc aaaacgcgcg ttctctccgg caaccaccac atcgcacaac
    36421 aatgccagtt cgcaacccgc accaagcgcg taaccgttga cggctgcgat gagaggtttg
    36481 ttgaaggcct gcaatcgcgc ccatagctgc gggcgtgtat cgtttaaggt ggccgcgaga
    36541 tctttttctg ccatttcgtt gagatcggcc ccagcggcaa aaaagcgtgc attaccggta
    36601 atcacacaga ccgaaatgct gctatcggta gccgcagctt ccagttcatt taccagttgc
    36661 gtcagaaggg cattatttag cgcattacgt gcggcgggac ggttaagggt cagcaacaat
    36721 acttgttgct gacggctgac gatcagttcg ctcatgccat ccccttcgcg tcaaagtcaa
    36781 ccaccacatc gctggtcagc ggcagtgcct ggcaactcaa cacatagcct gcggccagtt
    36841 catccggttc cagactgtaa ttggtttcca tcgccacttt gccacgcagc actttgcatt
    36901 tgcaggtcgc acagacgccg cctttgcagg catagggcag atcagccccc tggcgcaatg
    36961 ccgcatcgag aatgctttca tcgtcggcat taagcacgat ttcccgatcc cgcccatcct
    37021 gacgtacagt cactttttgt ccgtcacttt gcacgttaac gctacgtttg acgcgcgtgc
    37081 caggcgtatt aaaccgctcc agatgaatgg ttttatctgg cattcccagt gcttttaagg
    37141 cggcttccgt ctcatccatc atcgccgccg taccacaaat aaatgcctca tcataaagac
    37201 gaaaattaat gagcgaggcc ccaagtgact gtaatttttc accgtcaata cgcccgtgaa
    37261 gcagatcgct gtcgagggtt tcctgactga aaatgcacaa caactgtaaa cgctgaggat
    37321 atttgtcttt caggtctgcc agtgcctggc gaaacatcat gctctggctg gtacggttac
    37381 cgtagatcag ggtgaactga ctttcaggct cggtttgtaa agtggtggcg ataatcgcca
    37441 gcattggcgt aatacctgat cctgctgcaa tagccagata gcggccctgg cgttcggcct
    37501 gtggctgata gccgaaatgc ccctgcggca ccatgacctc cagcgtcata ccctggcgga
    37561 tgtgttcgcg ggcatagcgg gagaaacgtc cgccttcaat ggctttcacc gccacactaa
    37621 tttcgccagg cagatagctg cggcaaatgg agtaacaacg gcgtaattct tcaccatcaa
    37681 agctggcttt taaggtcaaa tgttgaccgg ggcgaaagcg atacgcctcc tgcaaaggct
    37741 ggggcaccgc aaaggtaatg gtcaccgcat cacgggtttc cggctccact tttgccaccg
    37801 ttaaggaatg aaacgttgtc atggcatcct caaatacatt tgaaataatc gaaaggttcg
    37861 cggcaactat cgcagcgata caatgctttg caggccgtgg aaccaaattc actgataagt
    37921 gtggtatgga cgctggcgca gcgcgggcaa cgtacttctg gcggcaaatg ggcatggcaa
    37981 ctgtgtccgg cgggcgggct aatgccatac tgtcgcagac gctcacgggc atcgggggtc
    38041 atccagtcgg tggtccatgc cgggtcgagt tgcagcacaa cctgaacggg ggtaaacccg
    38101 tgggttgtca ttgcctcacg tatcgcgcca atcaaatgtt ccgttgccgg acaaccggaa
    38161 taagtcggcg taaagccgat cacccatcct tctcccatct gtgtcacatt acgcaccatg
    38221 cctaaatcgg taatggtcag caccgggatc tccgggtccg ggatctggct gagtagcgcc
    38281 catatctcat gaacctgtgg cggtgcaatg gtggccagac gttgcatacc catctcctct
    38341 gttaccattg ctgaccgggc aagacacgct ggagatactg catttctgcc agcatgggtc
    38401 caaggtgttc ggtatgcagt ccttttttac cgccagtgcg atacgcctgc tcttgcggta
    38461 cgttcaatgt ggcttcgttg attccggcaa aaacttcggc ttcccacgct gcgcgtaaag
    38521 tgcgtggatc aaccgcaata ccctcttcac tcagtgcaat atcaatctcg tcggcatcga
    38581 acagttcggc agtaaaacgc cacaacttgt cgatggcctg ctgcatcttt tgccctgata
    38641 cgtcagtacc attgcccagt cgctccagcc agccacgact aaaacgcagg tgataacgcg
    38701 cttctttaat tgctttggca gaaatcgccg ccagttgcgg atcacggctt tccatcagac
    38761 gggtaaagag cgccacatgc catgcatcga tgaaatactg gcgtgcaatg gtgtcggcaa
    38821 aattgccgtt tggttgttca accaacaata agttgctgaa ctggcgctcg tctcgggtga
    38881 aggccagggt atcttcatca ccttctcccg ctaattcagc ggcatacgat aagaagttac
    38941 gtgcctgacc taataaatcg aggccaatgt ttgccagtgc gagatcgatt tccagttccg
    39001 gtgcgtgacc gcaccattcc cccaaccgct gggagagcac cagacagtta tcgcccaggc
    39061 gcaaggtgta agccgttaac tgattcattt ccgacctcac atgtgctcaa tgccatcagg
    39121 gatggtgtaa aacgttggat ggcgatagac cttgctttca gccggatcaa aaaattcacc
    39181 gcgttcttcc ggttgcgagg caacaatttc actcgccttc accacccaaa ttgaacatcc
    39241 ttcgctacga cgggtgtaag catcacgggc attttccagt gccatccgct catcggcagc
    39301 atgtaaactg ccaacatggc ggtgtgataa gccctgtttg ccacgcacga aaacttcgta
    39361 taacggccag taaacattac tcattttgaa tctccttatg cgaccttacg ggcaagttgt
    39421 ttttgtgcat gggccagcgc tgcttcccgt acccaggtac cttcttccca ggctttacgt
    39481 ttggcgtcga gcctctcctg attacaaatt ccgcgaccgt taattacttc gttaaactcc
    39541 tgccagtcga tctcaccaaa gcggtagtga ccgctttcag tgtcaaaatg cagatccggg
    39601 tcaggaacgg tcataccgag catttcaacc tgtggaatgg tgttatccac gaaacgctgg
    39661 cggagttcgt cgttggtgaa acgtttgatt ttccaggcga gacttctggc gctgtttggc
    39721 gagttatcat cgtttggccc gaacatcatt aaggctggcc accagaaacg gttaatggcg
    39781 tcttgcaaca tctgcttttg agcttcgctg ccctgcgcga gtgccatgca ggcttcaaac
    39841 ccctgacgct ggtgaaaact ctcttctttg cagattttca ccattgctct ggcatacggg
    39901 ccataagaag tgcgacataa cgccacctga ttgacgatcg ctgcgccatc caccagccag
    39961 ccgataacgc cgatatcagc ccaactcaat gtcggataat taaagatgga ggaatatttc
    40021 atccgtccgt cgagcatttt ttggtagatg tcttctctgg cgcagcccag tgtttcggcg
    40081 gcgctataga gatacagacc atgaccggct tcatcctgca ctttggccaa cagaatggct
    40141 ttacgccgca aggttggtgc gcgagtgatc cagttacctt caggcaacat accaacaatc
    40201 tcggagtgcg catgctgccc aatctggcgg atcagtgtct ttcggtaagc atcgggcatc
    40261 cagtcctgtg gctcgatagc cgtttcctgg gctatccgtt gctcaaagcg ttcttcttgg
    40321 gtcacgtcat cacctttacg attccagttt tcgtgaatta attaaaaaca cgaatcacat
    40381 tgttttgtga aagttacaca atggttaact aaaatcacaa attttgtatg cttgaactgt
    40441 gacggatttc gcaccctatt tttataaatc attatttatc agtgcattaa tgttttccgc
    40501 agttgctata cagatcgcat agttaacatt tcgttaaaag aacccttgct ttttatgatt
    40561 cgcgatttaa ctattagcaa cagaaatgtg aaacatctgg agagtagcga tgcagcagtt
    40621 agccagtttc ttatccggta cctggcagtc tggccggggc cgtagccgtt tgattcacca
    40681 cgctattagc ggcgaggcgt tatgggaagt gaccagtgaa ggtcttgata tggcggctgc
    40741 ccgccagttt gccattgaaa aaggtgcccc cgcccttcgc gctatgacct ttatcgaacg
    40801 tgcggcgatg cttaaagcgg tcgctaaaca tctgctgagt gaaaaagagc gcttctatgc
    40861 tctttctgcg caaacaggcg caacgcgggc agacagttgg gttgatattg aaggtggcat
    40921 tgggacgtta tttacttacg ccagcctcgg tagccgggag ctgcctgacg atacgctgtg
    40981 gccggaagat gaattgatcc ccttatcgaa agaaggtgga tttgccgcgc gccatgtact
    41041 gacctcaaag tcaggcgtgg cagtgcatat taacgccttt aacttcccct gctggggaat
    41101 gctggaaaag ctggcaccaa cgtggctggg cggaatgcca gccatcatca aaccagctac
    41161 cgcgacggcc caactgactc aggcgatggt gaaatcaatt gtcgatagtg gtcttgttcc
    41221 cgaaggcgca attagtctga tctgcggtag tgcgggcgac cttttggatc atctggacag
    41281 ccaggatgtg gtgactttca cggggtcagc ggcgaccgga cagatgctgc gagttcagcc
    41341 aaatatcgtc gccaaatcta tccccttcac tatggaagct gattccctga actgctgcgt
    41401 actgggcgaa gatgtcaccc cggatcaacc ggagtttgcg ctgtttattc gtgaagttgt
    41461 gcgtgagatg accacaaaag ccgggcaaaa atgtacggca atccggcgga ttattgtgcc
    41521 gcaggcattg gttaatgctg tcagtgatgc tctggttgcg cgattacaga aagtcgtggt
    41581 cggtgatcct gctcaggaag gcgtgaaaat gggcgcactg gtaaatgctg agcagcgtgc
    41641 cgatgtgcag gaaaaagtga acatattgct ggctgcagga tgcgagattc gcctcggtgg
    41701 tcaggcggat ttatctgctg cgggtgcctt cttcccgcca accttattgt actgtccgca
    41761 gccggatgaa acaccggcgg tacatgcaac agaagccttt ggccctgtcg caacgctgat
    41821 gccagcacaa aaccagcgac atgctctgca actggcttgt gcaggcggcg gtagccttgc
    41881 gggaacgctg gtgacggctg atccgcaaat tgcgcgtcag tttattaccg acgcggcacg
    41941 tacgcatggg cgaattcaga tcctcaatga ggaatcggca aaagaatcca ccgggcatgg
    42001 ctccccactg ccacaactgt tacatggtgg gcctggtcgc gcaggaggcg gtgaagaatt
    42061 aggtggttta cgagcggtga aacattacat gcagcgaacc gctgttcagg gtagtccgac
    42121 gatgcttgcc gctatcagta aacagtgggt gcgcggtgcg aaagtcgaag aagatcgtat
    42181 tcatccgttc cgcaaatatt ttgaggagct gcaaccaggc gacagcctgt tgactccccg
    42241 ccgcacaatg acagaggccg atattgttaa ctttgcttgc ctcagcggcg atcatttcta
    42301 tgcgcatatg gataagattg ctgctgccga atctattttc ggtgagcgtg tggtgcatgg
    42361 gtattttgtg ctttctgcgg ctgcgggtct gtttgtcgat gccggtgtcg gtccggtcat
    42421 tgctaattat gggctggaaa acttgcgctt tatcgaaccc gtaaagccag gcgataccat
    42481 ccaggtgcgt ctcacctgta agcgcaagac gctgaaaaaa cagcgtagcg cagaagaaaa
    42541 accaacaggt gtggtggaat gggcggtaga ggtattcaat cagcatcaaa ccccggtggc
    42601 gctgtattca attctgacgc tggtggccag gcagcacggt gattttgtcg attaatcggt
    42661 gaatgaaggg caacggcgaa tagttgccct tttatttcac caaattttgt gacgttgtca
    42721 cgttatgcat gatgtgtatc tgtattttca gggcacccac tgtatgaaaa gctggcacac
    42781 ctgccaaaca acctggcagg tgcaggcaat cccctttaca tcagtactga taatgtgaac
    42841 ctgactaaac cgcccacaga gcgcggttgc taacaagaac acaacatctg acgaggttaa
    42901 taatgggaag cccctctctg tattctgccc gtaaaacagc cctggcgttg gcagtcgcct
    42961 taagtttcgc ctggcaagcg ccggtatttg cccacggtgg tgaagcgcat atggtgccaa
    43021 tggataaaac gcttaaagaa tttggtgccg atgtgcagtg ggacgactac gcccagatct
    43081 ttaccctgat taaagatggc gcgtacgtga aagtgaagcc tggtgcgcaa acagcaattg
    43141 ttaatggtca gtctctggca ctgcaagtac cggtagtgat gaaagacaat aaagcctggg
    43201 tttctgacac ctttattaac gatgttttcc agtccgggct ggatcaaacc tttcaggtag
    43261 aaaagcgccc tcacccactt aatgcgctaa ctgcggacga aattaaacag gccgttgaaa
    43321 ttgttaaagc ttccgcggac ttcaaaccca atacccgttt tactgagatc tccctgctac
    43381 cgccagataa agaagctgtc tgggcgtttg cgctggaaaa caaaccggtt gaccagccac
    43441 gcaaagccga cgtcattatg ctcgacggca aacatatcat cgaagcggtg gtggatctgc
    43501 aaaacaacaa actgctctcc tggcaaccca ttaaagacgc ccacggtatg gtgttgctgg
    43561 atgatttcgc cagtgtgcag aacattatta acaacagtga agaatttgcc gctgccgtga
    43621 agaagcgcgg tattactgat gcgaagaaag tgattaccac gccgctgacc gtaggttatt
    43681 tcgatggtaa agatggcctg aaacaagatg cccggttgct caaagtcatc agctatcttg
    43741 atgtcgatga tggcaactac tgggcacatc ccatcgaaaa cctggtggcg gtcgttgatt
    43801 tagaacagaa aaaaatcgtt aagattgaag aaggtgcggt agttccggtg ccaatgaccg
    43861 cacgcccatt tgatggccgt gaccgcgttg ctccggcagt taagcctatg caaatcattg
    43921 agcctgaagg taaaaattac accattactg gcgatatgat tcactggcgg aactgggatt
    43981 ttcacctcag catgaactcg cgcgtcggac ctatgatctc caccgtgact tataacgaca
    44041 atggcaccaa acgcaaagtc atgtacgaag gttctctcgg cggcatgatt gtgccttacg
    44101 gtgatcctga tattggctgg tactttaaag cgtatctgga ctctggtgac tacggtatgg
    44161 gcacgctaac ctcgccaatt gcacgtggca aagatgcacc ctctaacgca gtgctcctga
    44221 atgaaaccat cgccgactac actggcgtac cgatggagat ccctcgtgct atcgcggtat
    44281 ttgaacgtta tgccgggccg gagtataagc atcaggaaat gggccagccc aacgtcagta
    44341 ccgaacgccg ggagttggtg gtgcgctgga tcagtacagt gggtaactat gactacattt
    44401 ttgactggat cttccatgaa aacggcacta ttggcattga tgccggtgct acgggcatcg
    44461 aagcggtgaa aggtgttaaa gcgaaaacca tgcacgatga gacggcgaaa gatgacactc
    44521 gctacggcac gcttatcgat cacaatatcg tgggtactac acaccaacat atttataatt
    44581 tccgcctcga tctggatgta gatggcgaga ataacagcct ggtggcgatg gacccggtgg
    44641 taaaaccgaa tactgccggt ggcccacgca ccagtaccat gcaagttaat cagtacaaca
    44701 tcggcaatga acaggatgcc gcgcagaagt ttgatccggg cacgattcgt ctgttgagta
    44761 acccgaacaa agagaaccgc atgggcaatc cagtttccta tcaaattatt ccttatgcag
    44821 gtggtactca cccggtagca aaaggtgccc agttcgcgcc ggacgagtgg atctatcatc
    44881 gtttaagctt tatggacaag cagctttggg taacgcgtta tcatcctggc gagcgtttcc
    44941 cggaaggcaa atatccgaac cgttctactc atgacaccgg tcttggacaa tacagtaaag
    45001 ataacgagtc gctggacaac accgacgccg ttgtctggat gaccaccggc accacacatg
    45061 tggcccgcgc cgaagagtgg ccgattatgc cgaccgaatg ggtacatact ctgcttaaac
    45121 catggaactt ctttgacgaa acgccaacgc taggggcgct gaagaaagat aagtgattgt
    45181 ttcagacaaa aaaacggcac caggtgccgt tttttactta tgagcgaacc agattaatac
    45241 cgtacacaca ccgacttagt ttcacaccaa ccgtccagcc agtcggggcc aaaatcacgg
    45301 cccgttcctg actgcttcat cccaccaaac ggtaagttag cgtcaattaa ggtatggctg
    45361 tttacccaca ccgtccctgc ctgtaagcga tcgctatatt ccagagcctg ggagagattt
    45421 tgcgtccaga cactggcagt taagccatat tccgtgtcgt ttgccagttg taacgcctct
    45481 tctccatccg ctactcgcac caggtttacc accggaccaa acacctcttc acgagttaag
    45541 cgcaatttag catcgggatt taccaccagc gttggcgcaa cataataccc ctctccggct
    45601 ggtccattcg acccgcgaat cagctctgct tgctgtgcct gcgcatcgtc gaggaatgaa
    45661 cacactttgt cgcagtgcgc acgagaaacc aaagggttaa tctgtgcaac aggtgacatc
    45721 cccggtccca cttacaacga ttttaccgcc tgctcaaatc cactaaccag cgtgtcaaac
    45781 aacggcgctt caatataaat tcgcgaactg gcggcgcata cttgcccttg attcaggaag
    45841 cttccggtca tcaagccttc aataacccat tgcggatcag catcttttaa tacaattgcc
    45901 gggtttttac cgcccagttc cagcgttaca cgcgttaagt gatcagcagc agttctggca
    45961 ataccttttc ccgtcgcggt tgaaccggta aaactgattt tcgcaacatg aggatgtgac
    46021 gtcagggccg cgccgcatac agcacctgac ccggtgacga cattaaaaac gccatcaggg
    46081 ataccagcct cgctggccag ttccgccacg cgcaacatcg tcagtggcgt ggtttccgaa
    46141 ggcttaatca cgattgaaca gcctgctgcc agtgctggca tcaccttcca cataccaatc
    46201 atcaacggaa agttccatgg cacaattccc gccactacgc caaccggctc tttacgcgtc
    46261 caggcctgat aacgcgcccc ctggggtaag ggaatcgaca agtccagcgt tttacccgcg
    46321 attttggtcg ttaacccggc ggtataacgc atccagttca gcgtacagcc cacttcaaaa
    46381 gcacgggaaa tggcaattga cttgccttgc tccagggttt ccagttgcgc cagctcctca
    46441 ctgtgctgtt ccaccagatc ggcaaaacgt agcagaatac gttcacgctc tgcgggtaat
    46501 cgcccggccc agcgacgcga gacaaaggcc cgccaggcag acatgactgc gttatctaca
    46561 tccgcttcgt tggcatcagc agtagacgca atttcttgcc cggtggccgg atcaaagatc
    46621 gccaatcgtt tttcactttg tgcggggcct ggacgaccat caatataaag accgtgttga
    46681 cgatcgagaa actgttggac ctggcttaat actgctacat gcggctctgt catcactttt
    46741 ccttattatt tacccagtgt gatttcagtg tacgcagcga caagaaaagt cacttttttc
    46801 gctgtgacat tcgctttgtc agctgtgaca agctccgcaa atcgtgacaa taaccgctta
    46861 tgtctctatg cctgagaaga cttatttcct atagtcaggc tcattagctt cgtttactgc
    46921 aacacaaatg caacaataaa aatacatttc acagagcgaa aacgtgccat gaaccccgca
    46981 gtggataatg agtttcagca atggctttcc caaatcaatc aggtatgcgg aaattttacc
    47041 ggacgcctgc tgactgagcg ttacacgggt gtactggaca cgcattttgc caaaggacta
    47101 aagctgagta ccgtgacaac cagcggggtg aatttatccc gcacctggca ggaagtaaaa
    47161 ggcagcgacg atgcctggtt ttacaccgtt tttcagctta gtggtcaggc aataatggag
    47221 caggatgagc gtcaggtgca gattggcgct ggcgatatta cgttactcga tgcctcacgc
    47281 ccctgttcgc tttactggca ggagtcttct aaacagattt cattactttt gccacgcact
    47341 ctgctggaac aatattttcc ccatcaaaaa cctgtctgcg cagaaagact ggacgctgac
    47401 ttacccatgg tgcaactcag tcatcgcctg ttacaggaga gcatgaataa tccggcactt
    47461 tctgaaacag aaagtgaagc tgcgctacag gcgatggtgt gtctgctgcg cccggtactt
    47521 catcagcggg aatctgttca acctcgtcgt gaacgtcagt ttcaaaaagt ggttacgttg
    47581 atagacgata atattcgcga agagatatta cgcccggagt ggatagccgg agagacaggt
    47641 atgtcagtac gtagtttgta ccgaatgttt gccgataaag gtttggtagt cgcgcaatat
    47701 attcgtaacc gtcgtctcga tttttgtgca gatgcgattc gccatgccgc agatgatgaa
    47761 aaactggcag gcatcggctt tcattgggga ttttctgacc agagtcattt ttcaacggta
    47821 tttaagcaac gctttgggat gacgccaggc gagtatcgac gtaaattccg ctaactaatt
    47881 ggcgttgtgt gtactgcaac gccaattagt taaaacagat atttgactat gcatttttca
    47941 gggcgaaagg caatttgaca ggagtttttg aatattcacc ttgtttcagg aaaatagatc
    48001 aatcgctttc tcctgtagcg ttatcccgat attgcggttt tgctattaaa attttcgcac
    48061 ccatttaccg tttatccctt gtacatattc ccccggtttg gcccgagcta cgagcttttg
    48121 cccggcgagt ttcgcgatat catctacaga cacattattt tgctttgcca gttgttggta
    48181 actcgcttta cgttcggcat taatgtcggt tactaatttc tctgtttcag catccgtttt
    48241 cagcgcaacc agataaccat aaaatgtttc acctacccgc ccctgagttc tggcttcatc
    48301 cagagtcaat gccattacat tgctgcttac cagcccaaca agaaacgcac aaagaagtaa
    48361 tgttttcttc atcatcgcct cagaaaagat cgctacgagt ttcaagcagc tcttcgacat
    48421 ctttgtctgc cttgatgatg atctcatgct caattttaac gttcatattg atagtgatcg
    48481 gttccttggg cgcagcgact tcaatacgcg gcgtacaggc agccagcata aaagatgacg
    48541 tcaacgcagc cagtaaaatt ttcattgttt ttcctcacac tcttttcctt gcggacagtc
    48601 atttcccggc agacgtgcgt tctgctccaa ccatgcctgg agattatcgc caaagcgtaa
    48661 actgcgccac agcgtaaaaa tattctcttc atgatagtaa ttaaggttta ccgtaccgct
    48721 tttaccatca acgcgactgg tacctgtaat gttggcctgc atggttaata aaccgagatt
    48781 atctaagtta atttttgtcg atgaccggct aatttccata tagcgcaacc agttaattgc
    48841 tgaacccgca gtcatattgt ctttcaccac cgcatctgcg gtgtctttat ccagccgcaa
    48901 ggtcattggc ccgctatttg ccagccaacc atctttcact atccattttt cgttgtttag
    48961 ccataacggc aatgcaccac taaatgcccc ggacatggcg aattgtttcg gattgacggc
    49021 gctaaccagt tcgctggatg agagattatt cagccgcaac agcgccgggt catgttgcgg
    49081 catacgtaat tgtttcatca gtacgttacc gcctaacaca tcgacgctaa catcagtcaa
    49141 cagcaagggt tcttcttcag tccacggata acgcccttgc aaatcagccg taatattttt
    49201 cgctgtcacc agattaatca cttcggcaat tcgcaacgta acggggccgc gagtccccag
    49261 atgccaggct ccatcggcaa aacggaaagg caggacaaaa tcgacaccgt taacctgatt
    49321 atctggcatc caggcactac cgcctttcaa cacgccgtgt cctcccgcgc ggaatccttg
    49381 ttcaggcgca gcagaaaatg caacctgagc atacagttca ccatcgcgta agttcatctt
    49441 ccagtcgggt ggcaccagcg gctggaatac agtcagtgat tgttttggcc accaggcgtt
    49501 accgcgcaga cgaataccgt cccagcgacc atttacccga accgggccaa tctcaccagc
    49561 atgtaaatcg cctttaaaga ggaaatacgt cggatcgcgc ccatcgacgc taaattttaa
    49621 ggttgatggc ggtaacacac tgccgccagt gaacagcgtt tgcccggcgt ccagtgacag
    49681 cgcgccgctg aaggagggat gctgtgcgtc acgtacccag acgacgggct tgtcgagaat
    49741 taatcgcggc ttttctaccg tcatcgtacc gtattgtaac tggtcgaaac cggtggagag
    49801 gtcggtcagc gtaatcgtgc tgtcatgcca ttcacctttt cctgcgacat cccaggtggc
    49861 attcatcggt gtaaaactgc cttttcccca gtagcgccac tgccagcggc ccgcgtcagg
    49921 gagaaaatca ttcgccagcc catccatatg cagcacgaaa tcgcccagtt cattttcatg
    49981 cgcctgcaaa atggcctgca aacgtccgtc aacaccacgt tgggtgactt ttacacccgc
    50041 taaaggccag cggatttcat cgatgtccag cgaatcgatg actcttccct ttgaacgaag
    50101 taacgcgcct ggctcaaagg tcagcgttgg gtcagacaga cttccactta gctgcgcagg
    50161 taaacgggca tataaaatga ggtccgcctg tttcgcttca ccggtcagct gcagaggcag
    50221 ctgactgtta tccatgctta attttcctgg gccaaaattt agcaccgcgt tgcctttacc
    50281 cgcttgcccc tgggtcagca cactcagtcg tccgctgatc agagcgttct caagccctgc
    50341 ctgccagttg tcgactttga cacccagtcg gccactcaaa ggaaaacctg catacggcca
    50401 actccagcga ccatcgctta cggtcaattg ttgacgagta atttgccacg gcaaatcgag
    50461 caacggatcg ccgttatccc gtgccagcac aatcaattgc ccgctatttt cctgccagtc
    50521 cagctctgca tccaccagtg acggttcctg cggcaagttt aacgtcgcag tagcatgccc
    50581 acttacagga agtccatccg gcacgagcgg catagtaaat tcccccacca gtttaaccgg
    50641 cggctgattt tcaaacgcga cgacatccag ttcgctgact gtaagttgtt gccctttcag
    50701 ctggccttga aatttaactt tttcgccctg ataacgcagt tgctggatat cagaggttaa
    50761 tgcgagagag agttttccct gccattcctg ccagggagaa aaaatcagtt tatcgatatt
    50821 gatccaggtg ttaggcagca tggactgcca ctgcgcgagg gtttttggag cagctggcga
    50881 ttgctccgtc tgcggcaatt tcgccagaca agcagaatca agttctaccg tgccgacgtt
    50941 caataaccag cggctgggat gtgaaaggct ggcgttggtg atatgcgcaa gctgacaatc
    51001 tcccaccaga taacggagat cgggaatgat taaaccttta cgcgtaatgc gtgggctttc
    51061 atctaatgca atacgtgtcc cgagcggtag ccagatgccc gccagcgtgg gaacccacag
    51121 cccgagcgtc atcagcagcg tcaacggcac aagaataatc agtaataaca gcgcgagaac
    51181 ggctttatat ttacccagca tgggtagtta atatcctgat ttagcgaaaa attaagcatt
    51241 caatacgggt attgtggcat gtttaaccgt tcagttgaag gttgcgccta cactaagcat
    51301 agttgttgat gaatttttca atatcgccat agctttcaat tatatttgaa attttgtaaa
    51361 atatttttag tggcttaaat gtgattcaac atcactggag aaagtcttat gaaactcgcc
    51421 gtttatagca caaaacagta cgacaagaag tacctgcaac aggtgaacga gtcctttggc
    51481 tttgagctgg aattttttga ctttctgctg acggaaaaaa ccgctaaaac tgccaatggc
    51541 tgcgaagcgg tatgtatttt cgtaaacgat gacggcagcc gcccggtgct ggaagagctg
    51601 aaaaagcacg gcgttaaata tatcgccctg cgctgtgccg gtttcaataa cgtcgacctt
    51661 gacgcggcaa aagaactggg gctgaaagta gtccgtgttc cagcctatga tccagaggcc
    51721 gttgctgaac acgccatcgg tatgatgatg acgctgaacc gccgtattca ccgcgcgtat
    51781 cagcgtaccc gtgacgctaa cttctctctg gaaggtctga ccggctttac tatgtatggc
    51841 aaaacggcag gcgttatcgg taccggtaaa atcggtgtgg cgatgctgcg cattctgaaa
    51901 ggttttggta tgcgtctgct ggcgttcgat ccgtatccaa gtgcagcggc gctggaactc
    51961 ggtgtggagt atgtcgatct gccaaccctg ttctctgaat cagacgttat ctctctgcac
    52021 tgcccgctga caccggaaaa ctaccatctg ttgaacgaag ccgccttcga tcagatgaaa
    52081 aatggcgtga tgatcgtcaa taccagtcgc ggtgcattga ttgattctca ggcagcaatt
    52141 gaagcgctga aaaatcagaa aattggttcg ttgggtatgg acgtgtatga gaacgaacgc
    52201 gatctattct ttgaagataa atccaacgac gtaattcagg atgacgtatt ccgtcgcctg
    52261 tctgcctgcc acaacgtgct atttaccggg caccaggcat tcctgacagc agaagctctg
    52321 accagtattt ctcagactac gctgcaaaac ttaagcaatc tggaaaaagg cgaaacctgc
    52381 ccgaacgaac tggtttaatc ttgccgctcc cctgcattcc aggggagctg attcagataa
    52441 tccccaatga cctttcatcc tctattctta aaatagccct gagtcagaaa ctgtaattga
    52501 gaaccacaat gaagaaagta gccgcgctcg ttgcgctaag cctgctgatg gcgggatgtg
    52561 taagtaatga caaaattgct gtaacgccag aacagttaca gcatcatcgt tttgtgctgg
    52621 aaagcgtaaa cggtaagccc gtgaccaacg ataaaaatcc gccagaaatc agctttggtg
    52681 aaaaaatgat gatttccggc agcatgtgta accgctttag cggtgaaggc aaactgtcta
    52741 atggtgaact gacagccaaa gggctggcaa tgacccgtat gatgtgcgct aacccgcagc
    52801 ttaatgaact cgataacacc attagcgaaa tgctgaaaga aggtgcacaa gtggatctga
    52861 ccgcgaacca gttaacgctg gcgaccgcga aacagacatt aacttataag ctggcggatt
    52921 taatgaatta atagctgcca cagctcccgg cggcaagtga ctgttcacta cagcgtttgc
    52981 cgttgggtaa tgcacacatc ccaatcgccg taccatccag ttgacgggca acagaaagcg
    53041 aaccgccgat cattgcacaa tttgcttctc cactactgga catcgacgct tttaaacctg
    53101 gcgctacgtg cgccgcagtc gcctgctgaa caggttcact actacacgcc gacaacaata
    53161 aagcggcaca ccctacccaa aacgctgctc gcatcttttc ttcctctgat cttcaagcca
    53221 aacgacaccg ccataaataa taggcagcac agagggcgac gtcgagagct gtcctgcgcg
    53281 ttgccccgca tttttacttt tttatggcta tttttttgcc ctctgtttga tcaaaacatt
    53341 cattacgctg atgtggggga cacaaaagcg aaaatgcaga agaaagccat ttgctaaaat
    53401 tgaaggatta ctactgggcg cgcagcaatt tcgtgcgccc ctcatttgcg caatgtaagg
    53461 gtgtcatatg attactattg acggtaatgg cgcggttgct tcggtggcgt ttcgcaccag
    53521 tgaagttatc gccatctacc ctattacccc cagttccacg atggcagaac aggctgatgc
    53581 ctgggccgga aacggcttaa agaacgtttg gggagacaca ccacgcgtag ttgaaatgca
    53641 gtcggaagcg ggtgctatcg ctaccgtgca tggcgctttg cagacgggtg ccctttcaac
    53701 atcgtttacg tcatcgcagg gtttgctgct gatgatcccg acgctgtaca aactggcagg
    53761 cgaactgacg ccatttgtcc tgcatgtagc ggcacgtacc gttgccacac atgcactttc
    53821 tatttttggc gatcattccg acgttatggc ggtgcgccag acgggttgcg cgatgttgtg
    53881 tgcggctaac gtccaggaag cgcaagattt tgctctcatt tcgcacatcg caacgctgaa
    53941 gagccgcgtg ccatttattc atttctttga tggtttccgc acgtcccacg aaatcaataa
    54001 aattgtcccg ctggccgatg acactattct tgatctcatg ccgcaggccg aaattgatgc
    54061 tcatcgcgcc cgggcgctca acccggaaca tccggtgatc cgcggtacgt ccgccaatcc
    54121 tgacacttat ttccagtctc gcgaagccac caacccatgg tacgacgcgg tctatgacca
    54181 tgttgaacag gcgatgaatg atttctctgc cgcgacaggt cgtcagtatc agccgtttga
    54241 atattacggg catccgcaag cggaacgggt gattatcctg atgggctctg ccattggcac
    54301 ctgtgaagaa gtggttgatg aattgctaac ccgtggcgaa aaagttggcg tgctgaaagt
    54361 tcgcctgtac cgtcccttct ccgccaaaca cttgctgcaa gctctgccgg gatccgtacg
    54421 cagcgtggcg gtactggaca gaaccaaaga acccggtgcc caggcagaac cgctctatct
    54481 ggatgtaatg accgcactgg cagaagcctt taataatggc gagcgcgaaa ctctgccccg
    54541 tgtcattggt gggcgctatg gtctttcatc caaagaattt ggcccggact gtgtactggc
    54601 ggtatttgcc gagctcaacg cggctaaacc gaaagcgcgc tttacggttg gtatttacga
    54661 tgatgtgacc aatctgtcac tgccgttgcc ggaaaacacc ctgccaaact cggcgaaact
    54721 ggaagccttg ttttatggcc ttggtagtga tggcagcgtt tccgcgacca aaaacaatat
    54781 caagattatc ggtaattcca cgccgtggta cgcacagggc tattttgttt acgactccaa
    54841 aaaggcgggc ggcctgacgg tttctcacct tcgcgtgagc gaacagccga ttcgttccgc
    54901 ttatctcatt tcccaggctg attttgttgg ctgccaccag ttgcagttta tcgataaata
    54961 tcagatggct gagcgtttaa aacctggcgg cattttcctg ctcaacacgc cgtacagcgc
    55021 agatgaagtg tggtcgcgct tgccgcagga agttcaggcc gtgctaaatc agaaaaaagc
    55081 gcgcttctat gtgattaacg cggcgaaaat cgcccgcgaa tgtggcctgg cggcccgtat
    55141 taataccgtc atgcagatgg cttttttcca tctgacgcaa attctgcctg gcgatagcgc
    55201 cctcgcagaa ttgcagggtg cgattgccaa aagttacagt agcaaaggcc aggatctggt
    55261 ggaacgcaac tggcaggctc tggcgctggc gcgtgaatcc gtagaagaag ttccgttgca
    55321 accggtaaat ccgcacagcg ccaatcgacc gccagtggtt tccgatgccg cccctgattt
    55381 cgtgaaaacc gtaaccgcag cgatgctcgc agggcttggt gatgccctcc ccgtttcggc
    55441 gctgccgcca gacggcacct ggccgatggg caccactcgc tgggaaaaac gcaatatcgc
    55501 cgaagagatc cccatctgga aagaggaact ctgtacccaa tgtaaccact gcgtcgccgc
    55561 ttgcccacac tcagctattc gcgcaaaagt ggtaccgcct gaagcgatgg aaaacgcccc
    55621 tgccagcctg cattcgctgg atgtgaaatc gcgtgatatg cgcgggcaga aatatgtctt
    55681 gcaggtggct ccggaagatt gcaccggttg taacctgtgc gtcgaagttt gcccggcgaa
    55741 agaccgtcag aatccagaga ttaaagccat caatatgatg tcgcgcctgg aacatgtcga
    55801 agaagagaaa atcaattacg atttcttcct caacctgcca gaaatcgacc gtagcaaact
    55861 ggaacgtatt gatattcgta catcgcagct gattacaccg ctgtttgaat attcaggtgc
    55921 ttgctccggt tgtggcgaga cgccgtatat taaattactg actcagctct atggcgaccg
    55981 gatgttgatc gctaacgcca ccggctgttc ttcaatttat ggcggtaacc tgccctctac
    56041 accgtatacc accgatgcca acggtcgtgg gccggcatgg gcgaactctc tatttgaaga
    56101 taatgccgaa tttggccttg gtttccgcct gacggtcgat caacaccgtg tccgcgtgct
    56161 gcgtctgctg gatcaatttg ccgataaaat cccggcggaa ttactgacag cgttgaaatc
    56221 agacgctacg ccagaggttc gtcgagaaca ggtcgcagct ttacgccagc aactcaacga
    56281 tgttgccgaa gcacatgaac tgctgcgtga cgcagatgca ctggtggaaa aatcaatctg
    56341 gctgattggt ggcgacggct gggcttacga tatcggcttt ggcggtctgg atcatgtatt
    56401 gagtttgacg gaaaacgtca acattctggt gctggatacg caatgctatt ccaacaccgg
    56461 tggtcaggcg tcgaaagcga caccgctggg tgcagtaact aagttcggcg agcacggcaa
    56521 acgtaaagcg cgtaaagatc ttggcgtcag tatgatgatg tacggtcatg tttatgtggc
    56581 gcagatttct ctcggcgcgc agctgaacca gacggtgaaa gcgattcagg aagcggaagc
    56641 gtatccgggg ccatcgctga tcattgctta tagcccgtgt gaagagcatg gttacgatct
    56701 ggcactcagc catgaccaga tgcgccaact cacagctacc ggcttctggc cgctatatcg
    56761 cttcgatcct cgtcgtgccg atgaaggcaa actgccgctg gcgctggatt cacgcccgcc
    56821 gtcagaagca ctggaagaaa cgttacttca cgagcaacgt ttccgtcgtc tgaattcgca
    56881 gcagccagaa gtggcagaac agttatggaa agatgctgca gctgatttgc aaaaacgcta
    56941 tgacttcctg gcacaaatgg ccggaaaagc ggaaaaaagc aacaccgatt aaatgctctg
    57001 gataag
//
LOCUS       DAZGAN020000018        54731 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGAN020000018 DAZGAN020000000
VERSION     DAZGAN020000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 54731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.018"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..382
                     /locus_tag="ACHKYO_RS06375"
                     /old_locus_tag="pgaptmp_001275"
     CDS             <1..382
                     /locus_tag="ACHKYO_RS06375"
                     /old_locus_tag="pgaptmp_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HGU4428153.1"
                     /translation="NGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGLGIS
                     VHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQ
                     RHKDFAFKPFFTNFNTIERIENYL"
     gene            379..858
                     /locus_tag="ACHKYO_RS06380"
                     /old_locus_tag="pgaptmp_001276"
     CDS             379..858
                     /locus_tag="ACHKYO_RS06380"
                     /old_locus_tag="pgaptmp_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HGU4428154.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(912..1862)
                     /gene="corA"
                     /locus_tag="ACHKYO_RS06385"
                     /old_locus_tag="pgaptmp_001277"
     CDS             complement(912..1862)
                     /gene="corA"
                     /locus_tag="ACHKYO_RS06385"
                     /old_locus_tag="pgaptmp_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HGU4428155.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2014..2070)
                     /gene="ysgD"
                     /locus_tag="ACHKYO_RS06390"
                     /old_locus_tag="pgaptmp_001278"
     CDS             complement(2014..2070)
                     /gene="ysgD"
                     /locus_tag="ACHKYO_RS06390"
                     /old_locus_tag="pgaptmp_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HGU4428156.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2232..2996
                     /gene="yigE"
                     /locus_tag="ACHKYO_RS06395"
                     /old_locus_tag="pgaptmp_001279"
     CDS             2232..2996
                     /gene="yigE"
                     /locus_tag="ACHKYO_RS06395"
                     /old_locus_tag="pgaptmp_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HGU4428157.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(3143..5305)
                     /gene="uvrD"
                     /locus_tag="ACHKYO_RS06400"
                     /old_locus_tag="pgaptmp_001280"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(3143..5305)
                     /gene="uvrD"
                     /locus_tag="ACHKYO_RS06400"
                     /old_locus_tag="pgaptmp_001280"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HGU4428158.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPAEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(5389..6105)
                     /gene="yigB"
                     /locus_tag="ACHKYO_RS06405"
                     /old_locus_tag="pgaptmp_001281"
     CDS             complement(5389..6105)
                     /gene="yigB"
                     /locus_tag="ACHKYO_RS06405"
                     /old_locus_tag="pgaptmp_001281"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HGU4428159.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(6105..7001)
                     /gene="xerC"
                     /locus_tag="ACHKYO_RS06410"
                     /old_locus_tag="pgaptmp_001282"
     CDS             complement(6105..7001)
                     /gene="xerC"
                     /locus_tag="ACHKYO_RS06410"
                     /old_locus_tag="pgaptmp_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HGU4428160.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTVVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(6998..7705)
                     /gene="yigA"
                     /locus_tag="ACHKYO_RS06415"
                     /old_locus_tag="pgaptmp_001283"
     CDS             complement(6998..7705)
                     /gene="yigA"
                     /locus_tag="ACHKYO_RS06415"
                     /old_locus_tag="pgaptmp_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HGU4428161.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(7702..8526)
                     /gene="dapF"
                     /locus_tag="ACHKYO_RS06420"
                     /old_locus_tag="pgaptmp_001284"
     CDS             complement(7702..8526)
                     /gene="dapF"
                     /locus_tag="ACHKYO_RS06420"
                     /old_locus_tag="pgaptmp_001284"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HGU4428162.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(8563..8766)
                     /gene="yifL"
                     /locus_tag="ACHKYO_RS06425"
                     /old_locus_tag="pgaptmp_001285"
     CDS             complement(8563..8766)
                     /gene="yifL"
                     /locus_tag="ACHKYO_RS06425"
                     /old_locus_tag="pgaptmp_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4428163.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8925..9067
                     /locus_tag="ACHKYO_RS06430"
                     /old_locus_tag="pgaptmp_001286"
                     /pseudo
     CDS             8925..9067
                     /locus_tag="ACHKYO_RS06430"
                     /old_locus_tag="pgaptmp_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            9229..9549
                     /gene="cyaY"
                     /locus_tag="ACHKYO_RS06435"
                     /old_locus_tag="pgaptmp_001287"
     CDS             9229..9549
                     /gene="cyaY"
                     /locus_tag="ACHKYO_RS06435"
                     /old_locus_tag="pgaptmp_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HGU4428164.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(9589..12135)
                     /gene="cyaA"
                     /locus_tag="ACHKYO_RS06440"
                     /old_locus_tag="pgaptmp_001288"
     CDS             complement(9589..12135)
                     /gene="cyaA"
                     /locus_tag="ACHKYO_RS06440"
                     /old_locus_tag="pgaptmp_001288"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HGU4428165.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            12522..13463
                     /gene="hemC"
                     /locus_tag="ACHKYO_RS06445"
                     /old_locus_tag="pgaptmp_001289"
     CDS             12522..13463
                     /gene="hemC"
                     /locus_tag="ACHKYO_RS06445"
                     /old_locus_tag="pgaptmp_001289"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HGU4428166.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            13460..14200
                     /gene="hemD"
                     /locus_tag="ACHKYO_RS06450"
                     /old_locus_tag="pgaptmp_001290"
     CDS             13460..14200
                     /gene="hemD"
                     /locus_tag="ACHKYO_RS06450"
                     /old_locus_tag="pgaptmp_001290"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HGU4428167.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            14222..15421
                     /gene="hemX"
                     /locus_tag="ACHKYO_RS06455"
                     /old_locus_tag="pgaptmp_001291"
     CDS             14222..15421
                     /gene="hemX"
                     /locus_tag="ACHKYO_RS06455"
                     /old_locus_tag="pgaptmp_001291"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HGU4428168.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            15424..16620
                     /gene="hemY"
                     /locus_tag="ACHKYO_RS06460"
                     /old_locus_tag="pgaptmp_001292"
     CDS             15424..16620
                     /gene="hemY"
                     /locus_tag="ACHKYO_RS06460"
                     /old_locus_tag="pgaptmp_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HGU4428169.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            17300..17437
                     /locus_tag="ACHKYO_RS06465"
                     /old_locus_tag="pgaptmp_001293"
                     /pseudo
     CDS             17300..17437
                     /locus_tag="ACHKYO_RS06465"
                     /old_locus_tag="pgaptmp_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(17545..18780)
                     /gene="aslB"
                     /locus_tag="ACHKYO_RS06470"
                     /old_locus_tag="pgaptmp_001294"
     CDS             complement(17545..18780)
                     /gene="aslB"
                     /locus_tag="ACHKYO_RS06470"
                     /old_locus_tag="pgaptmp_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU4428170.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(18927..19003)
                     /locus_tag="ACHKYO_RS06475"
                     /old_locus_tag="pgaptmp_001295"
     tRNA            complement(18927..19003)
                     /locus_tag="ACHKYO_RS06475"
                     /old_locus_tag="pgaptmp_001295"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18967..18969),aa:Pro,seq:tgg)
     gene            complement(19046..19132)
                     /locus_tag="ACHKYO_RS06480"
                     /old_locus_tag="pgaptmp_001296"
     tRNA            complement(19046..19132)
                     /locus_tag="ACHKYO_RS06480"
                     /old_locus_tag="pgaptmp_001296"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19096..19098),aa:Leu,seq:cag)
     gene            complement(19153..19228)
                     /locus_tag="ACHKYO_RS06485"
                     /old_locus_tag="pgaptmp_001297"
     tRNA            complement(19153..19228)
                     /locus_tag="ACHKYO_RS06485"
                     /old_locus_tag="pgaptmp_001297"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19193..19195),aa:His,seq:gtg)
     gene            complement(19287..19363)
                     /locus_tag="ACHKYO_RS06490"
                     /old_locus_tag="pgaptmp_001298"
     tRNA            complement(19287..19363)
                     /locus_tag="ACHKYO_RS06490"
                     /old_locus_tag="pgaptmp_001298"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19327..19329),aa:Arg,seq:ccg)
     gene            complement(19466..20851)
                     /gene="yifK"
                     /locus_tag="ACHKYO_RS06495"
                     /old_locus_tag="pgaptmp_001299"
     CDS             complement(19466..20851)
                     /gene="yifK"
                     /locus_tag="ACHKYO_RS06495"
                     /old_locus_tag="pgaptmp_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4428171.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(21042..21782)
                     /gene="wecG"
                     /locus_tag="ACHKYO_RS06500"
                     /old_locus_tag="pgaptmp_001300"
     CDS             complement(21042..21782)
                     /gene="wecG"
                     /locus_tag="ACHKYO_RS06500"
                     /old_locus_tag="pgaptmp_001300"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HGU4428172.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(21785..23137)
                     /gene="wzyE"
                     /locus_tag="ACHKYO_RS06505"
                     /old_locus_tag="pgaptmp_001301"
     CDS             complement(21785..23137)
                     /gene="wzyE"
                     /locus_tag="ACHKYO_RS06505"
                     /old_locus_tag="pgaptmp_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HGU4428173.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(23134..24213)
                     /gene="rffT"
                     /locus_tag="ACHKYO_RS06510"
                     /old_locus_tag="pgaptmp_001302"
                     /gene_synonym="wecF"
     CDS             complement(23134..24213)
                     /gene="rffT"
                     /locus_tag="ACHKYO_RS06510"
                     /old_locus_tag="pgaptmp_001302"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HGU4428174.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(24210..25460)
                     /gene="wzxE"
                     /locus_tag="ACHKYO_RS06515"
                     /old_locus_tag="pgaptmp_001303"
     CDS             complement(24210..25460)
                     /gene="wzxE"
                     /locus_tag="ACHKYO_RS06515"
                     /old_locus_tag="pgaptmp_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HGU4428175.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(25462..26592)
                     /gene="rffA"
                     /locus_tag="ACHKYO_RS06520"
                     /old_locus_tag="pgaptmp_001304"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(25462..26592)
                     /gene="rffA"
                     /locus_tag="ACHKYO_RS06520"
                     /old_locus_tag="pgaptmp_001304"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HGU4428176.1"
                     /translation="MIPFNAPPVMGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(26597..27271)
                     /gene="rffC"
                     /locus_tag="ACHKYO_RS06525"
                     /old_locus_tag="pgaptmp_001305"
                     /gene_synonym="wecD"
     CDS             complement(26597..27271)
                     /gene="rffC"
                     /locus_tag="ACHKYO_RS06525"
                     /old_locus_tag="pgaptmp_001305"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HGU4428177.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(27249..28130)
                     /gene="rfbA"
                     /locus_tag="ACHKYO_RS06530"
                     /old_locus_tag="pgaptmp_001306"
     CDS             complement(27249..28130)
                     /gene="rfbA"
                     /locus_tag="ACHKYO_RS06530"
                     /old_locus_tag="pgaptmp_001306"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGU4428178.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(28149..29216)
                     /gene="rffG"
                     /locus_tag="ACHKYO_RS06535"
                     /old_locus_tag="pgaptmp_001307"
     CDS             complement(28149..29216)
                     /gene="rffG"
                     /locus_tag="ACHKYO_RS06535"
                     /old_locus_tag="pgaptmp_001307"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGU4428179.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(29216..30478)
                     /gene="wecC"
                     /locus_tag="ACHKYO_RS06540"
                     /old_locus_tag="pgaptmp_001308"
     CDS             complement(29216..30478)
                     /gene="wecC"
                     /locus_tag="ACHKYO_RS06540"
                     /old_locus_tag="pgaptmp_001308"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HGU4428180.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(30475..31605)
                     /gene="wecB"
                     /locus_tag="ACHKYO_RS06545"
                     /old_locus_tag="pgaptmp_001309"
     CDS             complement(30475..31605)
                     /gene="wecB"
                     /locus_tag="ACHKYO_RS06545"
                     /old_locus_tag="pgaptmp_001309"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGU4428181.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(31661..32707)
                     /gene="wzzE"
                     /locus_tag="ACHKYO_RS06550"
                     /old_locus_tag="pgaptmp_001310"
     CDS             complement(31661..32707)
                     /gene="wzzE"
                     /locus_tag="ACHKYO_RS06550"
                     /old_locus_tag="pgaptmp_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HGU4428182.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(32719..33822)
                     /gene="wecA"
                     /locus_tag="ACHKYO_RS06555"
                     /old_locus_tag="pgaptmp_001311"
     CDS             complement(32719..33822)
                     /gene="wecA"
                     /locus_tag="ACHKYO_RS06555"
                     /old_locus_tag="pgaptmp_001311"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HGU4428183.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(34062..35321)
                     /gene="rho"
                     /locus_tag="ACHKYO_RS06560"
                     /old_locus_tag="pgaptmp_001312"
     CDS             complement(34062..35321)
                     /gene="rho"
                     /locus_tag="ACHKYO_RS06560"
                     /old_locus_tag="pgaptmp_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HGU4428184.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(35406..35507)
                     /gene="rhoL"
                     /locus_tag="ACHKYO_RS06565"
                     /old_locus_tag="pgaptmp_001313"
     CDS             complement(35406..35507)
                     /gene="rhoL"
                     /locus_tag="ACHKYO_RS06565"
                     /old_locus_tag="pgaptmp_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HGU4428185.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(35648..35977)
                     /gene="trxA"
                     /locus_tag="ACHKYO_RS06570"
                     /old_locus_tag="pgaptmp_001314"
     CDS             complement(35648..35977)
                     /gene="trxA"
                     /locus_tag="ACHKYO_RS06570"
                     /old_locus_tag="pgaptmp_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HGU4428186.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            36108..37373
                     /gene="rhlB"
                     /locus_tag="ACHKYO_RS06575"
                     /old_locus_tag="pgaptmp_001315"
     CDS             36108..37373
                     /gene="rhlB"
                     /locus_tag="ACHKYO_RS06575"
                     /old_locus_tag="pgaptmp_001315"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HGU4428187.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            37507..38991
                     /gene="gppA"
                     /locus_tag="ACHKYO_RS06580"
                     /old_locus_tag="pgaptmp_001316"
     CDS             37507..38991
                     /gene="gppA"
                     /locus_tag="ACHKYO_RS06580"
                     /old_locus_tag="pgaptmp_001316"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HGU4428188.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(39038..41059)
                     /gene="rep"
                     /locus_tag="ACHKYO_RS06585"
                     /old_locus_tag="pgaptmp_001317"
     CDS             complement(39038..41059)
                     /gene="rep"
                     /locus_tag="ACHKYO_RS06585"
                     /old_locus_tag="pgaptmp_001317"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HGU4428189.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            41276..41725
                     /locus_tag="ACHKYO_RS06590"
                     /old_locus_tag="pgaptmp_001318"
     CDS             41276..41725
                     /locus_tag="ACHKYO_RS06590"
                     /old_locus_tag="pgaptmp_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGU4428190.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTKYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            41924..42205
                     /gene="ppiC"
                     /locus_tag="ACHKYO_RS06595"
                     /old_locus_tag="pgaptmp_001319"
     CDS             41924..42205
                     /gene="ppiC"
                     /locus_tag="ACHKYO_RS06595"
                     /old_locus_tag="pgaptmp_001319"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HGU4428191.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(42292..43767)
                     /gene="ilvC"
                     /locus_tag="ACHKYO_RS06600"
                     /old_locus_tag="pgaptmp_001320"
     CDS             complement(42292..43767)
                     /gene="ilvC"
                     /locus_tag="ACHKYO_RS06600"
                     /old_locus_tag="pgaptmp_001320"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGU4428192.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            43917..44807
                     /gene="ilvY"
                     /locus_tag="ACHKYO_RS06605"
                     /old_locus_tag="pgaptmp_001321"
     CDS             43917..44807
                     /gene="ilvY"
                     /locus_tag="ACHKYO_RS06605"
                     /old_locus_tag="pgaptmp_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HGU4428193.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QALFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(44804..46348)
                     /gene="ilvA"
                     /locus_tag="ACHKYO_RS06610"
                     /old_locus_tag="pgaptmp_001322"
     CDS             complement(44804..46348)
                     /gene="ilvA"
                     /locus_tag="ACHKYO_RS06610"
                     /old_locus_tag="pgaptmp_001322"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HGU4428194.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(46351..48201)
                     /gene="ilvD"
                     /locus_tag="ACHKYO_RS06615"
                     /old_locus_tag="pgaptmp_001323"
     CDS             complement(46351..48201)
                     /gene="ilvD"
                     /locus_tag="ACHKYO_RS06615"
                     /old_locus_tag="pgaptmp_001323"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGU4428195.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAANGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(48266..49195)
                     /gene="ilvE"
                     /locus_tag="ACHKYO_RS06620"
                     /old_locus_tag="pgaptmp_001324"
     CDS             complement(48266..49195)
                     /gene="ilvE"
                     /locus_tag="ACHKYO_RS06620"
                     /old_locus_tag="pgaptmp_001324"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HGU4428196.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(49215..49478)
                     /gene="ilvM"
                     /locus_tag="ACHKYO_RS06625"
                     /old_locus_tag="pgaptmp_001325"
     CDS             complement(49215..49478)
                     /gene="ilvM"
                     /locus_tag="ACHKYO_RS06625"
                     /old_locus_tag="pgaptmp_001325"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HGU4428197.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(49475..51121)
                     /gene="ilvG"
                     /locus_tag="ACHKYO_RS06630"
                     /old_locus_tag="pgaptmp_001326"
     CDS             complement(49475..51121)
                     /gene="ilvG"
                     /locus_tag="ACHKYO_RS06630"
                     /old_locus_tag="pgaptmp_001326"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HGU4428198.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAAAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(51124..51174)
                     /gene="ilvX"
                     /locus_tag="ACHKYO_RS06635"
                     /old_locus_tag="pgaptmp_001327"
     CDS             complement(51124..51174)
                     /gene="ilvX"
                     /locus_tag="ACHKYO_RS06635"
                     /old_locus_tag="pgaptmp_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HGU4428199.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(51261..51359)
                     /gene="ilvL"
                     /locus_tag="ACHKYO_RS06640"
                     /old_locus_tag="pgaptmp_001328"
     CDS             complement(51261..51359)
                     /gene="ilvL"
                     /locus_tag="ACHKYO_RS06640"
                     /old_locus_tag="pgaptmp_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HGU4428200.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            51713..53233
                     /gene="yifB"
                     /locus_tag="ACHKYO_RS06645"
                     /old_locus_tag="pgaptmp_001329"
     CDS             51713..53233
                     /gene="yifB"
                     /locus_tag="ACHKYO_RS06645"
                     /old_locus_tag="pgaptmp_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HGU4428201.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(53258..53596)
                     /gene="maoP"
                     /locus_tag="ACHKYO_RS06650"
                     /old_locus_tag="pgaptmp_001330"
     CDS             complement(53258..53596)
                     /gene="maoP"
                     /locus_tag="ACHKYO_RS06650"
                     /old_locus_tag="pgaptmp_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HGU4428202.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            53715..54554
                     /gene="hdfR"
                     /locus_tag="ACHKYO_RS06655"
                     /old_locus_tag="pgaptmp_001331"
     CDS             53715..54554
                     /gene="hdfR"
                     /locus_tag="ACHKYO_RS06655"
                     /old_locus_tag="pgaptmp_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HGU4428203.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(54650..54725)
                     /locus_tag="ACHKYO_RS06660"
                     /old_locus_tag="pgaptmp_001332"
     tRNA            complement(54650..54725)
                     /locus_tag="ACHKYO_RS06660"
                     /old_locus_tag="pgaptmp_001332"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54690..54692),aa:Trp,seq:cca)
BASE COUNT        13453 a        14750 c        13837 g        12691 t
ORIGIN      
        1 aaatggcatt aattcaactc cctcgacagg ataccaacct cttatcctgc aaagcattaa
       61 aaccaaatta ctttcatcaa gactaaataa atttaatgac ttcgaggaca gttttaatgg
      121 cttaggaatc agtgttcatg atatttcagc tcaaaaaata tctcttttaa gttttcaaaa
      181 atacgctata ggatggagtg caacaattca ttttgtagca caggaccact tcggtcttga
      241 tgtaactgat atcaaaaaca agacttacag caaatataga ttttttcgca tatggttctt
      301 tttacaacgg cataaagact ttgctttcaa accttttttc accaatttta acactatcga
      361 aagaatcgaa aattatctat gaaaagcaaa ctccagaaaa ttatattatg tttatttctt
      421 ctatgctgca tctataattt atggacattg cgtcctgtac agattttgta tacatactct
      481 gatgctggca attcagtctt tctggtcgtt gatcatctgc catggactga cagcgataaa
      541 ataaattggt atctgaagca tcagaatgaa attaaaaatc aacatcccct accagaaggt
      601 agttggcata cctggtatgt cattgatatc ggtaatggct tcactgacta taaaaaatat
      661 atcgaagggc cgtatgaaga tctctactgt ttcccgacaa ttaagagcaa tgataattgc
      721 atcgtgaaaa attacttaat ggttattaat gaataccctt atcgtaacac tcatattggt
      781 attaacgatt ttactgagta tcaattaaca caagaaaata aaatagagcg agttttcaat
      841 ccgcatgact ttaaataaga caatttttag aaataagccg gataaaaaaa tagctttatc
      901 cggcactcat attacagcca gttcttgcgc ttaaagtaca gatacggtgc caggcccgcg
      961 aggatcataa agataatcgc gcctgggtag ccgaaactcc acttcagttc tggcataaac
     1021 tcaaagttca tcccatagct ggaagcaacg agcgtcggcg gcaggaatac cacggatacc
     1081 accgagaaaa ttttgatgat gcggttctgc tcgatgttga taaaacccat tgccgcctgc
     1141 atcaggaagt tcaccttctg gaacagggat tcgttatgcg gcagcaggga ttcgatatct
     1201 cgcaggattt cacgcgcctg ctccagttgc ccacccggta aacgcgcctt gcgtaccagg
     1261 aagttgagcg cacgctgggt atccatcaga cacaggcgaa ctttccagcc gatatcttcc
     1321 agttccgcca gcgtggagag cgcctcatcg tactcatcgc cctgatgccc ttccataatt
     1381 acccggctca actgctccag atcgctgtag atattttcaa tttcatctgc cagctgttcg
     1441 attttggttt cgaacagatc caacagcagc tcgtaggcgt taccgtccac cattgactgg
     1501 ctacgggcgc gcatacgata cagacggaat gcaggcagtt cacgctcacg cagagtaaac
     1561 agacgaccat cacggatggt aaatgccaca gtggagttac cggcgtgatc ttccgcatct
     1621 tcaaagaaga agaaggagtg aatatgcagg ccgtcatcgt cttcaaagaa acgtgccgat
     1681 gcttcgatgt cttccagttc cgggcgggtt gccaggctct ggccaagttc agattgtacg
     1741 cgcagtcgct cgtcgtcgtc cggttcgaca agatcaatcc atactgcatt tacaaggggt
     1801 tgtgactctt cgacttccag ccgggtcagt cggttatttt ccagttgaaa tgcgctcagc
     1861 atgaccggga ctcccaatgc ttaaaatatc ggacagttcg gtgggcacac agaaacaaat
     1921 tgggtttcag accattaaac agcctgactc agcgcgacgg gaaaaattga ggtcgctgac
     1981 aaccgctaag gctatcagca aaaagggata gccttaggag ttgatcctgg atgacaggat
     2041 agtgagccag tatctactgg gtgtgtccaa ggcgaatgtc ctcttagagt gatcgtgcgc
     2101 gcatgttacg ccagcaaaat tttgccgtca acacgcaaca gaacaccaca gaacaatagt
     2161 tgaacttcgc gctttttggt actcgtcaag gtcgctaaca ggataaaaaa atccaacatc
     2221 caggacctat aatggcgcat cggctactca ttggtaaagg aatgatcacc ttgaatctca
     2281 aacggatttt tctcgccctc accttacttc ccttgtttgc tgttgccgct gatgattgcg
     2341 cactctcaga tccgacgctg accgtacagg cgtataccgt taatcctcaa acagagcggg
     2401 tgaaaatgta ctggcaaaaa gccaatggcg aagcgtgggg aactttacat gctctgctgg
     2461 cggatatgaa tagtcagggt caggtgcaga tggcgatgaa cggcggcatc tatgatgaaa
     2521 gctatgcgcc gctcggtttg tacatcgaaa acggtcagca gaaggtggcg ttaaatctcg
     2581 cttcaggtga agggaatttc tttatccgtc ctggcggcgt gttttatgtc gcgggagata
     2641 aagtcggcat cgttcgtctg gatgccttca aaaccagtaa agagattcag tttgcggtgc
     2701 agtcagggcc aatgttgatg gaaaacagtg taattaatcc gcgtattcat cccaacgtcg
     2761 cctcacgtaa aattcgtaac ggtgttggga ttaataaaca tgggaatgcc gtgtttttgt
     2821 tgagccagca ggcgacaaat ttttatgatt ttgcctgtta tgccaaagcg aaactgaacg
     2881 ttgagcagct gctttatctt gatggcacga tttcacatat gtatatgaaa ggcggggcaa
     2941 tcccgtggca acgctatcct tttgtcacca tgatttccgt agagcggaaa ggttaaaacg
     3001 atagttttgt gtcagatact gaagatggcg cagatgccgg atgcggcgta aacgccctat
     3061 ccggcctaca tgacgttgca atttattgaa tatgaatgat tttttaggcc aaataaggtg
     3121 cgcagcaccg catccggcaa cgttacaccg actccagccg ggcgtatgcc gccaccagcc
     3181 atttaatacc ctggccctgg aatgccacct gcaaacggct atgctcgccg ctgccttcca
     3241 tattgacgat ggtgccttca ccaaacttag cgtggcgtac acgctgaccg agcttgtagc
     3301 cgctgtcgtt ctcgaccatc ggcgtcccca tccgttgatg gctgaccgga cgacttaccg
     3361 tagcgcgcag gcgcacctct tccacacact cttccggcag ctcgccgata aagcgcgacg
     3421 ggcgatggta aacctcttta ccatacagac ggcgggtttc cgcgtaggtc agcgtcagtt
     3481 tctgcatcgc gcgggttaca ccaacgtagg ccagacggcg ttcttcttcc agacgcccgc
     3541 cttcatccag cgacatctgg cttgggaaca taccctcttc cataccgaca ataaacacct
     3601 gcgggaactc caggcctttc gccgagtgca gcgtcatcaa ctgcactgca tcctgccagg
     3661 tatccgcctg cccctcgccc gcttccagcg ccgcatggga gaggaatgcc tgcagcggca
     3721 ttaaatcttc atcttcttcg ttgtagctga actggcgcgt tgccgtcacc agttcctcta
     3781 agttttcgat acgcgtctgg cctttttcgc ccttctcctg ctcgtacatg gtgcgcaggc
     3841 cggagtcttt aattacccgg tcagtctgta catgcagcgg catatcggca gtttcttgcg
     3901 ctaaggcatc gattaattcc ataaatcgct gtaaggcgct ggcagcacgt ccggcgaggg
     3961 ctttttcctg caacagttca cgacatgcct gccagagtgt taactggcga tcgcgcgatg
     4021 tctgacgtac cacgtccagc gtccggtcac caataccccg cgttggcgta ttcaccacac
     4081 gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg cagatacgag agcgcgtctt
     4141 tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat acggtacggc atactggcct
     4201 gcaacagtgc ttcttccagt acacgcgact gggcgttact gcggtagaga atggcgcact
     4261 cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg gttaaccaca aaacgcgctt
     4321 catcgagttc gttaaaagcg caatagaggg aaataggctc accgtccgcg ccatcggtcc
     4381 acagtttttt acccagacgc ccgttattgt tttcaatcag ggcgttagcg gcgctcagaa
     4441 tattgctggt agagcggtag ttttgctcca gacgaatagt ttcggcaccg gggaaatcat
     4501 taaggaaacg ctgaatattc tccacctgcg ccccgcgcca gccgtagatt gactggtcgt
     4561 catcaccgac gatcatcact ttgccggtgt cgcccgccag caggcggatc cacgcgtact
     4621 gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt ggtaaaacgt tcgcggtagt
     4681 gttgtaggat atgcggcttg ttaagccaca gttcgtgagc gcgcagcagc agctcggcga
     4741 agtccaccag gcccgcgcgg tcacacgctt cctgatacgc ctgatacacc ttctgccagg
     4801 tctgctccgc cggattaccg tagctttgaa tatgatgcgg acgcaggcct tcatctttct
     4861 ggctgttgat gtaccacatt gcctggcgcg gcggccactg cttctcgtcg aggttcatgg
     4921 ctttgatcag acgcttaagc aggcgcagct ggtcctcgct atcgaggatc tggaaatcct
     4981 gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg gtgcgccagc ccgtggaagg
     5041 tgccgaccca catgccgccc tggctggtgc ccatcagttg cccgatacga tgacgcatct
     5101 ccgccgccgc tttgttggta aacgtcaccg ccataatcga gtatggcgag cagttttcca
     5161 cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt cttaccactg cccgcgcccg
     5221 ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc gcgctgtttg tcattaaggc
     5281 tgtcgagcag gtaagaaacg tccattggca ccgccgcgta aaaataggtt gggcggagaa
     5341 gcgcaacgcc ctccgccaaa aagctgggta tatatacaga tttgctgatt atatcagcga
     5401 ggtcagagat gccaaccggg aaatttccag atgcggcagt aaacggctgt cccaggtttg
     5461 catcagatcg ccattttccg gtctgatcca acaggcctgc attccgctgc gaattgcccc
     5521 acccacgtca gtggtgagat cgtccccaac atgtaagatc tcgccgatcg gcacgttgag
     5581 tttttccgca gccagaaagt acatatcgct gaacggtttt gagcgcccgt gcgggccagc
     5641 gcgcagcaca aactcaaaat aatcccccaa accaaacagt tccggctggg cgttaccgtt
     5701 ggtgatcgcc accagcggcc atttcttcgc cagctgtttt aaggtgtcgt gagtttgctg
     5761 cgggacgtcg attcggctgc gccatttggc aaagttgatc attgctgcgt gtgcgcctgc
     5821 actggcttct tcggcactca gcccggcgtc gagcatcgct tgttcaatcg aacgaaaacg
     5881 ccaacgcgtc acgtcgtgat aaatctcggg ttccgcttcc cgtaccgcct ggcgcaggcg
     5941 ttgcagatct tcattctgga agctgcgcag cgccggatga taattttgca caaaggtaag
     6001 cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg taaagggtat catccaggtc
     6061 aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa cgcattattt cccccgtttg
     6121 gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt gaaaatcaag atgagtatag
     6181 atttgcgtgg tggagaggtt ggcatgaccc agtagctcct gcacaccacg aagatcgccg
     6241 ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt tatgtgggtg aacgtgatta
     6301 ttcagccctt gttttatgcc ccattcggca aagcgtttct gcacattacg cgcggagata
     6361 cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc taccaaacag gtcgcgcaaa
     6421 tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa tcggcaggcg gcgctctttg
     6481 ctgcctttcc ccataaccca cacttcacca gactccaggt cgaggtgttt aatatccagc
     6541 cccaccagct cagaaagacg cagacccgcg ccgtacatca cttccagcat tgcacggtcg
     6601 cgtacagcga ggggatcatt gatatcaata tccagcagcc gattcatatc gtcgacgtcg
     6661 atgtttttcg gcagatgacg cggcgctttc ggtgccgaaa cacctttcgc cgggttagct
     6721 ttgagttcgt tctggctgac cagccagtca aaaaagctac gtagcgcaga aagccgtaac
     6781 gccagacttg ctgctcccag ccctttacgg cgactgcgta cagcaaaatt gcgcaccacc
     6841 gtcacatcac attgctgcca gctttgcagg ccgttttcgc tggcaaaatt gatgatcgcc
     6901 tcaagctgac gctggtagtt aagcagggtt atcgggctaa gctggcgctc cacgctcaga
     6961 taacgtaggt agcgttctac atcggtgtgt aaatcggtca tacgcgttca atccaacgct
     7021 ccagaagctc cggcaacatc agcgcaattt catgaagtaa ctgcgttcct tgcccttgtt
     7081 gatagtgact ggcatcgcga ctggtaaaca gcacaacacc caaatcagca tcgcttccca
     7141 gcatcgacat cgccaccgat cccactgctt tcgcttcagg tagcaccacc agcagctcag
     7201 gtccgttaag cggcccaaga tagtgctgtt cctgccccaa acgctgaata cgcagcggtt
     7261 cgaaagactg acggcttaat gccagatgag tgtggttcga cggcgcacct aagcgccagc
     7321 gatccggaaa caggcgcaga ctcgcacctg ccaggccgag atcgcgcgcc cagcggtgaa
     7381 agcgcatcag catatcgtcg agactgctgg cggcggtgag actgcgctgc aggtagagta
     7441 ggcgataaaa caggccttcg ttggcgatag cctgttccat caacagcgcc atgttctctt
     7501 ccagaacatg aatatgatta cgtgcgcggg ccatgtgcca ctcgaccaac gaaacggtgc
     7561 cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg cgcattacgg ataaaaaact
     7621 caggattttt aatcagataa tcgacaaccg cccggtcatc aagctccgtg agtgtttcct
     7681 gcagttcttc ccctggttgc ttcatagatg aataaatccg tcgtagacat gtaccgccgg
     7741 gccagtcata tataacgggt gacccggacc tttccaggcg atatcaagac gaccgccggg
     7801 gagttccacg cgtacttctt cggccagcaa accttgctga atccctactg caaccgccgc
     7861 acacgcgccg ctgccgcagg cctgggtttc tcctgcccca cgctcataaa cgcgtaaacg
     7921 aatatgctcg cgcttaacca cttgcataaa accgatattg gcgcgctccg gaaaacgctc
     7981 gtggctttcc agaacaggac caagcgtttc taccgccgcg gtatcgacat catcgacctg
     8041 aatcacgcaa tgcggatttc ccatcgacac cacgccgcat aagattgtct gctcggcggc
     8101 gcgcataata taggtctttt ccgctttgtt agcgcgaaac ggcacggcgg aaggttcgaa
     8161 gttgggttcg cccatattta cgcggaccag atcatcatcg gtgacggtca gaaccatccg
     8221 cccgttggcg gtgctgacgc ggatatcacg cttattggtc agtcctttca gacgcacaaa
     8281 acgggcaaag cagcgcgcac cgttgccgca ctgcgccact tcactgccat cagcattgaa
     8341 aatgcgatag tgaaaatcca gttcaggatc atacggcggc tcaaccacca gcagttggtc
     8401 aaaccctacc cccaggtgcc gatcagccag gcgacgaatc agctccggtg aaaaaaagac
     8461 attctgcgtt accgcgtcga cgaccataaa atcgttgcca aggccatgca ttttcgagaa
     8521 ctgcatcatt tactccaatc acgcgggtac agaaactgac ttttagtaat tcacctggga
     8581 tggaccatcg ccagtggcgc gatcgttttt atccggcacc gtggattgcg tttgcgtctc
     8641 taccggtttg gtcggcggcg gtgcgttttt atctgcaggc gggaaataga gcggaccttt
     8701 cagaccgcag cccgtcaggc tgaaaagagt aagtaataca gtgagtgcct taaacacgtt
     8761 tttcattatg gattgcctgt aaagttcatc gctttctgct ttctatcatc gcaggagaag
     8821 acgtaaaagc aagcgtttag cgactcttta tgaagtctga aaagtgagga aatgctgcga
     8881 gtataacgct tactcgtggg gatttatgtt ttgctggtag tttcgtgatc ggcacctcgc
     8941 gccaggatgg ctttatgcac tatcgcaatg tagtttgttt gttggctttt ttagttccgt
     9001 cagcgtgagg atgctcgctc ggtaaacctg atcataatgt ttaccagaaa cagggaaagg
     9061 cgtggtgtaa ctgcgcccta atgaagagac aaatctttcg ctacactgag acatcattat
     9121 tccggtgacg gatgaatcgt gccggaacgc agccgcagag gtgcaaactt tgaagccctg
     9181 accccatgcc gctatactgc cgccatcact taaaaacagg atacaacaat gaacgacagt
     9241 gaatttcatc gcctggctga tcaactctgg ctgaccattg aagaacgcct ggacgactgg
     9301 gatggcgaca gcgatatcga ctgcgaaatc aacggcggcg tactgaccat tacctttgaa
     9361 aacggcagca aaatcattat taaccgccag gagccgctgc accaggtgtg gctggcaacc
     9421 aaacagggcg gctaccattt tgacctaaaa ggcgatgaat ggatttgcga tcgcagcggc
     9481 gaaaccttct gggatttgct ggaacaggcg gcgacacagc aagcgggtga aacagtcagt
     9541 ttccgctaag attgcatgcc ggataagcct cgctttccgg cacgttcatc acgaaaaata
     9601 ttgctgtaat agcggcgtat cgtgatcctg attggcaggc ggcatgttac cgatagattt
     9661 tgtgcggaac ggaatcacct gttcacgacc atcaaccttc acaatctgat agaactgcgg
     9721 caggttgaag ttgatgaagc ttgagccgta ggtgaagcgg tcgtgcgatg acgagtagaa
     9781 gcgactgacg tcacgcacca gttcctcttt gctgccttcg cagtggtgat acacctcaac
     9841 ccggttgctt tcgtcgagaa tgtagatatt aaagccattc tcgtcttgcg tttcttcgaa
     9901 gaagaactgg atgatccctt cactagcaaa gccgtccacc accgccggta atttgacgtg
     9961 attggtttca acctgcactg acaagccgtg cagtttgtta tgcgaaatag cgccataaaa
    10021 ctcgatggcg ttttccagtt tttgtaccga tacattcagg cgttcgaaga acaaccccca
    10081 ggtttgacca gaaacgcgca gcgccttgaa acgcccggtt tcctggcggg tgctggaaag
    10141 gcgcagttca atacactcag aaaccagttg ctgcacgcga gtacggatta acccgcgcag
    10201 atgctggcta taacagaaga cttccacgct atctggcggt gcggcgtcct gatgcatttt
    10261 gccgagaata gttttcaggg cttcgatcat cgattgctcg ccgttgaagt gcagcgtacg
    10321 cacttcgttc cacgagttgc ggtacagcag gtcaacgcta cctaccaggc aattttgatt
    10381 ctcgccaaag ctgaagacat ccagcttacg gaaatcaaaa tgcaccacct gattgcggaa
    10441 cgccgctgtc gggtcatatt ccaggttaac gataatcgcc agatggcgga tctcacacgg
    10501 gctgtagagc gccttcggtg tcggtgcagg taagcgcagc gggaaatggt gcgacacgtc
    10561 ggcgaccatc tcctgcaact taggcaaatc gacaatgccg ttacctttaa tatacaaacg
    10621 ggtgcgcgag gtcagcaggc cgttaaacca tgcccacgcc accagtttgt tcaggtaacg
    10681 gttatattcc agcggctgat ggctgatgat cgactcaata tttggcgcgc ggttatacag
    10741 ataccaacct gaacggttag cacggcccgg cggcacataa ataaaggtca gattcggttc
    10801 cgagagatcg ggtgaaatct gcgggtttac cagcgtcact ttacctggta atgcttcaaa
    10861 cgcggcatac agcttacgcg tcagcacgcc gatatcctgc ggactggcgg agacgctaag
    10921 gttattgcga cgcgcaaagc ggatcagatt acggtagctc tgcatcatcg cgtcgagcaa
    10981 ctcgttgtgc gcctcacgca cctgatcaat cttccagtta gcgcggttat cgagcattgc
    11041 cagacgagct tcgtcccaac cccactcgct cactaactgg ctcaacactg cgcgacgcca
    11101 gcctacgcag gcgcgttcac ggctgagctt ttcgcacact tttaaataga agcagcgacg
    11161 tactaaatcc agacgggtaa aatcttcaat cgccgtcagg tattcagtaa cacgctccag
    11221 catcatgcag tatggatcga gaccaaacga tacaatctcg ccgtcgtgca aacgctgttt
    11281 gatatctttc gccagcagac gtgggttcgg gtattcccag gaataggctt ccagcagcag
    11341 tgttttcagt accgctttgt atggggaatc gatactcttg tagagctgcc aaaggctggc
    11401 accaaagtac tcttcagcag aaagcgagct taagccaccg agatccagcc attcatttgg
    11461 cgtcagcacg ccctgcgcgt aaagcgtcat cacatagtcg tcgtaatgct cttcttcgtc
    11521 gcacggcacc atattccaca gaatacgctt accggcgaga cgcacggcgg tacgataaaa
    11581 ttcgtcaagc agcagtatat gctgggtgga gccacaatct tcgcccccca ggctgccgct
    11641 ttcattatga cggaagcggt tttcatcaat caggaagaag ctgacttcca cacccagcga
    11701 ggcggcccag ctttccagca gactacattt acgttgtagt aactggcgct cttcgctatc
    11761 gagccaggat tgatgacaga cccagatatc caggtcagag gaacaacttt gccctacgga
    11821 cgaggtgctg cccatggtgt atacaccagt aattggaagc tcacctttcg gtgggtcctg
    11881 tactgacatt ccacgataca gttcaagctc gttcaggtag tggcgttgag tttcatcagg
    11941 cgtgtaaagg caaatgcctt tgggaacgtt accatcaagg taacccggca ttagcggatg
    12001 gtgatagtgc aacaatgtcg gcagtagact gtagacctgt tggaatgcag gccccatagc
    12061 agcaagcgcg cgatccacac gcaattgatt tatggcatcc agtctctgtt tcagagtctc
    12121 aatatagagg tacaagacgt atcgcctgat ttgctacccg tcatgactgt gattccgcca
    12181 acatcaacgg taacacgcgg cattcgggat atttcgtatg tcaaaggtaa ccgttaccac
    12241 ttttcgcgcc tggttttttt agtttcacga cgaaaaaatg gtctaaaacg tgatcaattt
    12301 aacaccttgc tgattgaccg taaagaaaga tgcgctacat acaagtgtag caccgtttat
    12361 tctctgtaaa ttccttatta cgacggcgtg aaacgcctgt caggatccac tgccagacct
    12421 cattttacgg tttgcgcagg cgtctacgtt tcaccacaac actgacatca ctctggcaag
    12481 gatgttagga tggaccacgg atgataatga cggtaacaag catgttagac aatgttttaa
    12541 gaattgccac acgccaaagc ccacttgcac tctggcaggc acactatgtc aaagacaagt
    12601 tgatggcgag ccatccgggc ctggtggttg aactggtacc gatggtgacg cgcggcgatg
    12661 tgattcttga tacgccgctg gcgaaagtag gcggaaaagg cttatttgta aaagagctgg
    12721 aagtcgcgct cctggagaat cgcgccgata tcgccgtaca ttcaatgaaa gatgtgccgg
    12781 ttgaattccc gcaaggtctg gggttggtca ctatttgtga gcgtgaagat cctcgcgatg
    12841 cctttgtgtc caataattat gacagtctgg atgcgttacc ggcaggcagt atcgtcggga
    12901 cgtccagttt acgtcgccag tgccaactgg ctgaacgccg cccggatctg attatccgct
    12961 ccctgcgcgg caacgtcggc actcgcctga gcaaactaga taacggcgaa tacgatgcca
    13021 tcattcttgc cgtagccgga ctaaaacgtt taggtctgga gtcccgcatt cgcgccgcgt
    13081 taccacccga gatttctctt ccggcggtag gacaaggtgc agtcggtatt gaatgccgcc
    13141 ttgatgatac gcgcactcgc gagctgcttg ccgcgctgaa tcaccacgaa actgcactgc
    13201 gcgttaccgc agaacgcgcc atgaataccc gtctcgaagg tggatgtcag gtgccaattg
    13261 gtagctacgc cgagcttatt gatggtgaaa tctggctgcg tgcgctggtc ggcgcgccag
    13321 acggttcgca gattattcgc ggtgaacgcc gcggtgcgcc gcaaaatgcc gaacaaatgg
    13381 ggatttcgct ggcagaagag ctactgaata acggcgcgcg cgagatcctc gctgaagtct
    13441 ataacggaga cgccccggca tgagtatcct ggtcacccgc ccgtctcccg ctggagaaga
    13501 gttagtgagc cgtctgcgca cactggggca ggtggcctgg cattttccgc tgattgagtt
    13561 ttctccgggt cgacaattac cgcaacttgc tgatcaactg gcggcgctgg gggagagcga
    13621 tctgttgttt gccctctcgc aacacgcggt tgcttttgcc caatcacagc tgcatcagca
    13681 agatcgtaaa tggccccgac tacctgatta tttcgccatt ggacgcacca ccgcactggc
    13741 gctacatacc gtaagcggac agaagattct ctacccgcag gatcgggaaa tcagcgaagt
    13801 cttgctacaa ttacctgaat tacaaaatat tgcgggcaaa cgtgcgctga tattacgtgg
    13861 caatggcggt cgtgagctaa ttggggatac cctgacagcg cgcggtgctg aggtcacttt
    13921 ttgtgaatgt tatcaacgat gcgcaatcca ttacgatggt gcagaagaag cgatgcgctg
    13981 gcaatcccgc gaggtgacga tggtcgttgt taccagcggt gaaatgttgc agcaactctg
    14041 gtcgctgatc ccacaatggt atcgtgagca ctggttacta cactgtcgac tattggtcgt
    14101 cagtgagcgt ttggcgaaac tcgcccggga actgggctgg caagacatta aggtcgccga
    14161 taacgctgac aacgatgcgc ttttacgggc attacaataa ctctcataac aggaagccat
    14221 aatgacggaa caagaaaaaa cctccgccgt ggttgaagag accagggagg ccgtggacac
    14281 cacgtcacaa cctgtcgcaa cagaaaaaaa gagtaagaac aataccgcat tgattctcag
    14341 cgcggtggct atcgctattg ctctggcggc gggcgtcggt ttgtatggct ggggtaaaca
    14401 acaggccgtc aatcagaccg ccaccagcga tgccctggct aaccaactga cggcattgca
    14461 aaaagcccag gagagccaaa aagccgagct ggaaggcatt attaagcaac aagctgcaca
    14521 gcttaagcag gcgaatcgtc agcaagaaac gctggcaaaa cagttggatg aagtccaaca
    14581 aaaggtcgcc accatttccg gcagcgatgc taaaacctgg ctgctggctc aggccgattt
    14641 tctggtgaaa ctcgccggac ggaagctgtg gagcgatcag gacgtcacga ccgctgcagc
    14701 gttgctgaaa agtgcagatg ccagcctggc ggatatgaat gacccgagtc tgattaccgt
    14761 tcgtcgggca attaccgatg atatcgccag cctttctgca gtttcgcagg tggattatga
    14821 cggcatcatc cttaagctta atcagctttc aaatcaggta gataacctgc gtctggccga
    14881 taatgacagc gatggttcgc cgatggattc cgacggtgaa gagctttcca gttccatcag
    14941 cgaatggcgt atcaatctgc aaaaaagctg gcagaacttt atggacaact tcattacgat
    15001 tcgccgtcgt gatgacaccg ccgtaccgct gttagcgcca aatcaggata tctatctgcg
    15061 cgaaaatatt cgctctcgcc tgctggtcgc agcacaagct gtaccgcgtc accaggaaga
    15121 gacttatcgc caggcgctgg agaacgtctc cacctgggta cgagcttact acgatactga
    15181 tgatgccacc accaaagcgt tcctcgacga ggtggaccag ttaagccagc aaaatatctc
    15241 gatggatctt ccggaaaccc tgcaaagcca ggcgatgctg gaaaaactga tgcagacccg
    15301 cgtgcgtaac ctgctggcac aaccggcagc ggggacaacg gaagctaaac ctgcacctgc
    15361 acctgcacct gcacctgcac cgcaaggtga tactccggca gccgcgccgc aaggagaata
    15421 accatgctaa aagtgttatt actctttgtt ttgctgattg cggggatagt ggttggcccg
    15481 atgattgccg gtcatcaggg ttacgtgctg atccagactg acaactacaa tatcgaaacc
    15541 agcgtcacgg gcctggcgat tattttgatt ctggcgatgg tagtgctgtt tgccattgag
    15601 tggctactgc ggcggatctt ccgcacaggc gcacacaccc gtgggtggtt tgtcggacgt
    15661 aagcgtcgcc gtgcccgtaa gcagaccgaa caggcgctgc tgaaactggc ggaaggcgat
    15721 tatcagcaag ttgaaaagct gatggcgaaa aatgccgatc acgcggaaca accggtggtg
    15781 aactatctac tggctgccga agccgcgcaa caacgtggtg atgaagcacg cgccaaccaa
    15841 catctggaac gcgcagcaga gctggccggc aatgacacca ttccggtaga aatcacccgt
    15901 gtacgtctgc aactggcccg taatgaaaac catgctgcac gccacggcgt ggataagctg
    15961 ctggaagtta cgccacgcca tccggaagta ttacgtctgg cggaacaggc gtatatccgc
    16021 acaggtgcat ggagttctct gctggatatc atcccatcaa tggcgaaagc ccatgttggt
    16081 gatgaagaac atcgtgcaat gctggaacaa caggcatgga ttggcctgat ggatcaggcg
    16141 cgtgccgata acggtagtga aggtttgcgt aactggtgga aaaaccaaag ccggaaaacg
    16201 cgtcatcagg tagcgttgca ggtggcaatg gcggaacatc ttattgaatg tgacgatcat
    16261 gatactgccc agcaaattat catcgatggc ctgaaacgcc agtacgacga tcgcctactg
    16321 ctgccgattc ctcgactgaa aaccaacaat ccggaacagc tggaaaaagt gctgcgccag
    16381 cagatcaaaa acgtcggcga tcgcccgctg ttgtggagca cactgggcca gtcactgatg
    16441 aagcacggag aatggcagga agcatcgctc gccttccgcg cggcgctgaa acaacgtccg
    16501 gacgcctacg attacgcatg gcttgccgac gcgctggaca gactgcacaa gccggaagaa
    16561 gctgcagcga tgcgtcgcga cggtttgatg ttaacgttgc agaacaaccc accacagtag
    16621 ttccttctca cccggaggca agcacctccg ggacctttct gatgcataaa aaaacgcctg
    16681 ctcttattac ggagcaggcg ttaaaacagg tctgtatgac aacaagtggg tgcttcactc
    16741 aacgttgtgt ccatggtgtc tgatgaggca taagcgacat ctgtcagtgg acgataagca
    16801 ccgtaaacgg ctctgcgtca ttccggagtt tatgaggcac taaggcgaac ataagagatg
    16861 gaatgagcat ctacccgttt attatgccac agagaatcgg gaaataacat cccttaacac
    16921 ttgttatgag ataattctgt aatcctcttt gcttcctgag taataacttc ctgagtgaat
    16981 atttaacctg agcttgatcc tacacatact tattatgaat gataaaattc attcaattaa
    17041 taacacatat attaattgcc gttaaaacta aaaaaaagca tcaataatca acgcgatata
    17101 ataaacctgc cttacatatc aactgcgcca gaggtaggat tgaaaacgct ctcctgattt
    17161 tccaattcat tttctggata aataaataat ttatttttgt cactattatt tatgtaatca
    17221 tcctgtcagg gagagggatc tcaattatca atgcttaatt acgtcatcat tttgatgaca
    17281 tgaaaccgga gcattttgca tggaattttc gttttcaccc aaacttcttg ttgttgctgt
    17341 cgccgccgct cttcctctca tggccagcgc agcagatacc ccgtcaactg ccaccgcacg
    17401 caaaggcttt gccggatacg atcacccaaa ccagtatcca cagaacgctg caatttattg
    17461 aatttgcacg atcatgtagg ccggataagg cgtttacgcc gcatccggca atcaaccgca
    17521 ggcggccaca aaatggccgc ttttttactt attcaccacc agcaaatgtg cctgcataat
    17581 gtcactggcc gggcgaccat gcgccagcaa atcagccatt gctttaagat atggcggtag
    17641 atggcggaaa taacgctgat acccggcaca caaataattc agccccggtt tgccgctggc
    17701 atcgagcatg aagcggtgtt tcgggcagcc tccccagcac gcttttaaca cgttacaact
    17761 gcgacactgc gccggtaatt gcttaaattt atcttcacca aacacctgct gttgcgggga
    17821 atcgatcatt tctgcaattg tttgctgatg catattcccc agccgatatt gcggataaac
    17881 atagtgatcg caggcgtaaa cgtcgccgtt gtgctcaaca atcaccgagc gcccacaggt
    17941 tggctgatga tggcaaaccg cacccggcgc accgacaaaa ttggcaaacg cccattcgat
    18001 attcatcacg aaaatcttgc cgacgtcgcg tttgatccag tggtcgaata tcgccaccag
    18061 aaattcacca aattcatcgg ggcgcaccga ccattccgtc agttccccct gaatatcacc
    18121 aggcgcatgt agtttcagtc catcgctggc agctgtttca tcagccaggc gctcgaccac
    18181 cggaataaac tggatgaatt cgactcccgc atcgcacaaa aaatcataaa cctgcaacgg
    18241 ttgctgcgcg ctggtgcggt tgacgcagac cagcacgtta tagtcgacat gatgtttttg
    18301 cagcagcgtc agggcacgca tcaccagctt atgcgttggt ctgccacctt tggtcacgcg
    18361 atattgattg tggatctcag ccggaccgtc cagcgataac ccaacaagaa aatgattttc
    18421 tgccagaaat gcacaccatt tatcatcgag cagcacgccg ttagtctgga agctgttact
    18481 tatcttcctg ccagcaccgt atttcgcctg taactttacg gcacggcggt aaaaatccag
    18541 cccgagtaac gttggttcgc cgccctgcca ggtaaaagcc acttcgtttt gcggttcgct
    18601 ggcagcgata tagtgacgga catacgcttc cagcgtgtca tcgtccatat gcgtgactgg
    18661 cttttcgcgg taaagggatt gtttttcgag ataaaaacag tagtcacagt tcagattaca
    18721 atcggaacca ctcggtttcg ccatcacatg aaaagcacgc gttggaacct gttgcagcat
    18781 ggtcactcct tgcctgaagt catccttcaa tacgttaagg gcgaaatcat accgtggggc
    18841 aatgagttat tacgggcgtt tattgcgaag ggagggtgac gaaatgcaca gaaaacaaaa
    18901 aaccccgccg aagcggggtt caaaattggt cggcgagaga ggattcgaac ctccgaccca
    18961 ctggtcccaa accagttgcg ctaccaagct gcgctactcg ccgaaatact gctttttgaa
    19021 tttttagttc aattctttaa agtcgtggtg cgaggggggg gacttgaacc cccacgtccg
    19081 taaggacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc gcattgtcac
    19141 aacttctaat aatggggtgg ctaatgggat tcgaacccac gacaactgga atcacaatcc
    19201 agggctctac caactgagct atagccacca ctacaaatct tgttacgcgg tattactacc
    19261 accgcagctc aagcgccggg actaaatggc gcgcccgaca ggattcgaac ctgagacctc
    19321 tgcctccgga gggcagcgct ctatccagct gagctacggg cgcttagcgc cgttgcgggg
    19381 gtggataata cggacttccc acccctctgt ctagtccctt tttaaataaa ttgcgcgttt
    19441 ggttacggtt tgtgcgtttt gctgcttatt cctccagttt atgcgctttc ccgtggcgat
    19501 taaggccaaa aactttataa atcgccgtca ccgccagcat aaagatgatg ccaacaaaca
    19561 gcgacatacg cgtatcttca ttaaagtaca tgccgatcaa aacgcaaatc aggaatgcca
    19621 ttgttacgta gttggcccac gggaacagga ttgagcggaa cggatggctg gcaatcgccg
    19681 ctttatgtgc acgccgaaaa cgcagctggc ttatcaatat cacaaaccat ggcaccatcc
    19741 ccggaagcac gctggcactg tagacgtaga caaacacacg ctgcggattg ggaatgatgt
    19801 agttcaggca tgagccaatt agcagaatag caatagatac tgccacaccc gcaactggta
    19861 cgccgtgacg ggaaactttc gccatcgccg ccggtaactg acggtttttc gccagtgcgt
    19921 agagcatacg tccgcaactg tacatgccgc tgttacagcc agagagcgca gccgtcagca
    19981 ccacaaagtt gataatgccc gccgctgcgg taataccgat tttggcaaaa gtcagtacga
    20041 acgggctgcc gttgctgcca atttcattcc acgggaagat ggtgacgata acgaaaatcg
    20101 cgcctacgta gaaaatcagg atccgccaca gcactttgcc tacggcactg cgcagcgtca
    20161 cctgcggatt cttcgcttca ccggcagtaa tgccaatcag ctccacgccc tggtaggacg
    20221 ccaccacaat acacagcgcg gtcaggaacc ctttccagcc acccgcaaag aaaccgccat
    20281 gctctgtgag attgctaaaa ccaatcgact gcccgccatt gccaaagcca aagaaaatca
    20341 cgcccaggcc aatgacaatc atcacgataa tcgtggtgac tttgatcatc gcgaaccaga
    20401 actcgatttc gccgtacaac cgcaccgccg ccagattcgc caacgccacc agcgccactg
    20461 cgatcaatgc gggtatccac tgcgccatct ccgggaacca gaactggaca taaacgccaa
    20521 tggcggtgat ttctgagatc cccacggcca tccacataaa ccagtaagac caggcggtga
    20581 gataaccaaa gaacgggctc atataacgat gcgcataaac ggcgaacgaa ccggtaaccg
    20641 gttcgaggaa caacatttcg cccattgaac gcatgatgaa aaagacgaat agcccggcga
    20701 tgatataggc caacaatacg gatggcccgg cccatttcaa ggtactggcg gcccccataa
    20761 acaggccgac gccaatggtg ccccccaggg cgatgagttc gatatgtcga gcttccagcc
    20821 cacgctgtag ctctggtttg ttatctgcca taaatcctcg tgttgtgttt gcatgctttc
    20881 cggtgttacc ggttatcgtt atgggtacat cgagtgttgc gaatgttttc gtaattcagg
    20941 agaaatggca aataaagcat taaaaatttg aatgctttgt gtaataaaaa agcagacagg
    21001 cgacggagtg accactccgt cgctttacaa agagaggaaa atcagaggtt gccggtgtag
    21061 tgccagcgta aataacgcag caaacgaagc tgacgcttaa tgcggctcgg ctgcgaaagc
    21121 aggcggtaga gccactccag tcccagcgtt tgccagattt tcggtgcgcg ttttacgtga
    21181 ccggtgaaaa catcgtaagt cccgccaacg cccatataca gcgcatctgg atgcaccaga
    21241 cggcagtcgc gcatgaagat ctcctgcttt ggcgatccca tcgcaacggt gacgatttgc
    21301 gctccgctgg catgaatgcg ttcaaacagc gcctgacgct gctcgggttt aaaataacca
    21361 tcctgactgc caacgatatt cacattccat tggttgcgca gtttagcttc agtttgcgcc
    21421 agcacttcag gtttaccgcc cacaagaaat accggcgtcc cttctttgcc tgcgcgcgcc
    21481 atcagctctt cccagagatc ggcaccggca acgcgggaaa cctgcgcctg cgggtacttt
    21541 ttacgtactg aacgtacaac gctgatgcca tccgcatatt taaattcggc agcgttaatt
    21601 aactccctga cctcggcgtt atcttcaatg gtcagcattt tttcggcatt aatggcaacc
    21661 agcgttccct gcttaagctg cccgtcagca aacagataat cgagggcgtg ctgcatatca
    21721 cgccaaccaa tcaactgtaa gccacgcagc gtataggtcg gtgccgtggt gttgttattc
    21781 attgttatcc ttcaacctgc gtccggagcg atgattttgt acgtttatga atgagtccgg
    21841 cgctttcaaa aagccagtac aacagttttg cgatcatcag acatgcgccg aagaccacga
    21901 taaaaaagac tacgcgtgag acaaacgaat ccagcccttc acgtgccagc acgatcatat
    21961 tgaagatcgc cccaaagcag aaactgtgca atatcgcagc cttatagcga ttaggctcgc
    22021 ggttgcccag ctcatacagc cagtcgaacc atttgatgat cagaccaacc acgatcgccc
    22081 cgagcgggat gaacaacgcg ccgcccatca ccaccagtga gcctataagc gtaggcgaga
    22141 tcgccagtcc ggagtggtta ttcagcactt cccaggtaaa gtagttggct gagttcagca
    22201 ccatactcgg gcgacccggc cacagccagg aagggataaa gacatagaaa tcgcggacaa
    22261 ttggagccag gccctggaag tcgatgttgt cgtagttctg caacagcaac gccagattct
    22321 cccacggcga gaaggtgtcg cgagtgagat agagaaacgt atagaacgct tcatcgccgc
    22381 tcacattcat tccatagcgt tttagcgcca gccagaacat gcccacaatc cccagcacgc
    22441 ccgccgccgc cagcatccac aacgaaatcc agccgcgaat aatgccaata aacaggaaga
    22501 tagcgaatgc gatgatgata ttggcgcgag tgccgccgac aatcatataa gtcagcaagc
    22561 caaaggcgac cgtgctgacg aggaaaaaca gccacgcttt gctatcctgg cgcagaaagt
    22621 agaccaccag catcgccggg atgaaaaagt aaaagaagcg ttttaacgcc acgccggaga
    22681 cttcactgga gaagatctga ctgtaggagt taagccggaa cagcaaaaag ccgttgtgca
    22741 tgaagaagat gccgacgctt accagcgcga tgcccatcag gatcacccac gtaagattgg
    22801 tctccacgcg gttcatggta aacagcggac ggcgcggtac atcagcaacg cgtttgcgta
    22861 ggcgggtttt gtaggtgaca tagtaaaccg cgtagaagca gcccgcagaa agcaacgcct
    22921 gcaacaagat ttctggcggc gcgacaccaa catcaaagcg aaataccagc acgctggtca
    22981 gcgggaagcc gaagaaaaag gtgagcaaaa acagcaatga aaagaagaca ttgaagttaa
    23041 agcgcacacg gcgaaactca aaccaggtca gcgtggcaat aaacagcgtg cagagcagcc
    23101 agaccacaaa caggccactg aattgcagca gactcatgcg ccctccccgg cggcaatcgc
    23161 caacgcccgc tgccagcctt gtagatagtt agggctaaag aaggcaatgg tgtttttatc
    23221 caccgacgcc aactggcgct gcgcttcacg cacaatatcc tcgttgagat cgtcggtagt
    23281 aaacagcact ggcaaatgct gttccgtcat atcctgccag aacggatttt cccggttaag
    23341 cacacaagga atgcccgcct gaatcagtaa gcacagcgta ccaatgccct gctggcgagc
    23401 aaaaataaag taaccaagat cgcactgacg aagtagcgcc agataggcgt caaattccag
    23461 tttttcgctc aaaatttgca gattttcttc gctgaataac tccagccccg cctgacgaac
    23521 ttcctcaatg tacgcttcgt tattaggcgg atatcccatc ggcaccacca cttttaccgt
    23581 atcgccaaat tgctgatgaa cggcacgtaa agcggcgaca tgctcattgc tgaggtcgcc
    23641 ggagttcccc accagaatgg tcatttttcc ttcacgttgc cgatcgttcg ccatcgtatt
    23701 gagagaaggg tccatccgcg tcgggaagta cagcagttct ccccgcacct ttgggtgcgt
    23761 tttggcaaaa aagctcaaat cgccgcgggt ggcaaataca cagccgactc gcttttgcgc
    23821 caggcgacgt agtgggtaaa aaagcttata tctcaagcca ctggaaagct catacaggtc
    23881 tgccccccag atatgccaga aaaactggct gggcttaatg ccaccactca gcagagccag
    23941 ccacagtgtg ggattgaact gaccgtggaa aaaaaatcgc tgctgacggt tagcttttgc
    24001 tttcgcgatg accgcttccg ccagcgattt tttcccaggg aaaaattgca cagaaagcgc
    24061 cggacagcta tcacttaagc cgtcgtcctt gccaacaacc ataaactcgc gcgcgtgctc
    24121 gctcgtcgca gccagcgcgt cattgaaaaa ccgcaaaacg gttcggttat ggtgagggat
    24181 atccgatccc agtacgtgaa tcagtacagt catgcccgcc tacgccagag taaaaacacg
    24241 ccacaacaaa gagaaaaata gacgatataa gttgccatat atgcctgcgc cgcgcccagt
    24301 gcaccatgcg cagggattag ccagtgggca aataccatca ataaagtgaa ctggctgatt
    24361 tccgccagaa tataaaaccg caatgacgct ttggcgatca ccagataacc aaagacataa
    24421 gcgcccactt ttaacacatc acccactaac tgccaggcaa agagatcgcg catagcggta
    24481 aatttattcg acaacagcag ccagatagca aaatcacgca gcagccagac ggtaaaactc
    24541 gccgccgcca ctgccggtaa gacgaatttc agcgatttaa ccacttcccg ggtgatatcg
    24601 cgcttttccg ttagccgcga caacgtgggc agcaaatata cgctgaacga tgccgtaata
    24661 aattgcaggt aggcatcgga aatactgctc accccttgcc agatccccac ttcatcccag
    24721 ctatactgcg ccgccagcag tttacgcatc atgatgtaag caacaggcaa ggtcaccgac
    24781 gtaatcaacg ccatgagcgt aaatttgctc aactgccctg ccagaccgtt atcccagctg
    24841 ggtttcagat agcttaacgg gatgacacca cgtttgatca acatgatagc ggcaggaatt
    24901 accaccagcg cgggaatcag cgccagaccc agcaacgccc cttcataacc gcccaaacgg
    24961 taactgacgt agtacgcgag aacgccaatc aagctgccga caatcagcga taacgcatta
    25021 cctgcggcat cgcgaaagcc tttcatcagc gccagtaaca ggttgcccca ggcgatcccc
    25081 atttgcacca gcgccactaa acgcaccagc ccctgatagt cggtattacc aaacaatccc
    25141 tggctgattg gcgcagctgc cagcacaaaa accagcgcca tcagcgtaga gaaaccaagt
    25201 accatcgctg atgaagtgcc gaccacgcgg cgcagctgtt gcggattatc atggtactgg
    25261 gcaacgtatt tggttacacc gttaaagatg ccagctccgg caagcacgcc gagcacggta
    25321 atcaactggc ggaaatttgc cgccagccca agccccgccg gaccaaatga cacagccagc
    25381 aacttaccga ccagtaaccc ggcaccaatc ttgaccagtg tactggccgc cgtccacaag
    25441 gacgcttttg ccaacgacat atcaggagaa gtagttcaac aaagtcgcaa ttaccgtacg
    25501 ctgattgacg ggcgacaggt tgtagaacag cggcaggcgc agcaggcgct cgctctcttt
    25561 ggtggtgtag cgatcttcac cgtggaactc accaaagcgt tcccccgcag ggcaaccgtg
    25621 cagcggaatg taatggaaca ccgccatgat ttccgcttct ttcagaaagt taatcaacgc
    25681 gctccggtca tcaatatccc gcagtttaat gtagaacata tgcgcgttct gcacgcagcc
    25741 atcgggaatc gacggcagct cgatacgccc ggctttcgcc agaggcgcta acgcatcgta
    25801 gtagttttgc cacagcgcca gacgttgctg gttgatacga tccgctgctt ccagttgcgc
    25861 ccacaggtac gcagcttgca gatcggacat caaatagctg gagccaatat cgcgccaggt
    25921 atatttatcg acctgaccac ggaagaactg gctgcggtta gtgccctttt cacggatgat
    25981 ctcggctcgt tcgattaacg ctttatcgtt aatcagcgtc gcgccgcctt caccacccgc
    26041 cgtatagttt ttggtttcat ggaagctaaa gcagccaata tgaccaatgg ttcccagtgc
    26101 acgccctttg taagtggaca tcacgccctg agcggcatct tctaccacaa acagattatg
    26161 ctttttcgcc aacgccataa tggtgtccat ttcgcaggcc acacccgcgt aatggaccgg
    26221 cacgataacg cgcgttttgt cggtgatcgc cgcttcaatc agcgtttcgt cgatgttcat
    26281 ggtgtccggg cgaacatcca caaaaacgat ttttgcgcca cgcagcacaa aggcattggc
    26341 ggtggagaca aaggtgtagc tcggcatgat cacttcatcg ccaggctgga tatcgagcag
    26401 cagcgccgcc atctccagcg aagcggtgca ggacggcgtc agtaacactt tggcgctgcc
    26461 aaaacgttgc tccagccact gctggcagcg acgggtaaaa ccgccatcgc cacacagttt
    26521 gccgctaccc attgccgact gcatatagtc gagttcggtt cccatcaccg gcggtgcgtt
    26581 aaatggaatc atgtgatcac ctgtataacc agtacgcggt gctttctaca ttcgcaccac
    26641 tttgtatgta tcgtttaagc gcggcggtgt tgcccatttg ggtcgccacc cgcaaagttg
    26701 ttttaccgcg agcatacgcc cagtttagcg ccgtttgcat cagctcagca cctgcaccgc
    26761 gtccagccag caggccaatt cgcgcatctg tcgcattgag ttcccgtaaa gagacataac
    26821 cgcgaatacc gccggacgcc gcacgtaaaa tcagacattg atgatcaaag gtgccgcgca
    26881 cggcattttc aatccactgt gcataaaagc gtccgctggc gtcaggcgca taccacggcg
    26941 cacgaaaacg gctttgcgca aatgcggcgc tggctaactg acgtaatgcg ggaatatcgg
    27001 tctcttgtgc cactacagca ccactatcac tgacattgtt cacgggtagt gccaaatcaa
    27061 cttcaccttc taccagggag aatcccagct gttgcagggc atccagttca cccgtatttg
    27121 atgccgcaat tttggcctgc acccgtgacc acggcgctaa cacgtctggc gtcaggagcg
    27181 gtgcttcaga cgtaatgcgc acgatggcgc tgttaacacc aaagaaggcg ttttcccagg
    27241 ttagtggctc aatactggcg cggacgggca cgaagtaact ccagcagata ttggccgtag
    27301 ccagttttcg ctaatgaact ggcagcacgc ttcacaccct cgtcatcgag ccagccgtta
    27361 cgccaggcga tctcttccag gcaggcaatc ttaaagccct ggcgtttttc tactgtctgc
    27421 acaaaggtac tggcttcaat caggctgtcg tgagtgccag tgtccagcca ggcaaatccg
    27481 cgcccgagca gttcaacggt caggttgccc gcctcgaggt acatctggtt gatggaggta
    27541 atctccagtt caccacgctc cgacggcttc acctgctttg cgtactccac gactttactg
    27601 tcgtagaaat aaagcccggt caccgcccag tttgacttcg gctgttttgg cttttcttcc
    27661 agcgagatag cgcggaaatt gtcgtcaaac tccaccacgc caaagcgttc cgggtccatc
    27721 acctgatagc caaataccgt cgccccttcc gtgcgcgccg caacatgacg cagcttcgga
    27781 ctgaagccct gaccgaagaa gatgttatcg cccagcacca gacaagaagg ttcaccgttg
    27841 aggaaggttt caccgatgat aaaggcctgc gccagaccgt ccgggctggg ctgttcggcg
    27901 tattccagct ggataccgaa ctcactacca tcgcccagca ggcgctggaa ataaccttta
    27961 tcttccggcg tagtgatgat gagaatttcg cggataccgg ccaacatcag caccgacagc
    28021 ggatagtaaa tcattggctt atcgtaaatc ggcaacagtt gcttcgatac gccgcgcgta
    28081 atcggatgca atcgggtgcc ggaaccgccc gccaggataa tacctttcat gtttgcctcc
    28141 gctgaaaatt agcctttcag acctaaacgc tcgccctgat agctgccgtc ctgcacctgc
    28201 ttccaccagc tttcattagc cagataccac tgcaccgttt tacgcattcc actttcaaag
    28261 gtttcctgtg gcagccaacc aagttcacgg gcaattttcg aagcatcaat ggcataacgc
    28321 agatcatgcc ccggacggtc agcgacaaag gtgatcaagt cacgatagtg cgccacgccg
    28381 tgcggcttgt tcggagccag ttcttccagc agctcgcaaa tggtttccac aacatcgaga
    28441 ttcttacgct cgttgtgacc accaatatta taggtttcac cgactttccc ggtggtcgcc
    28501 acgcaataca gcgcgcgggc gtgatcttcc acatacagcc agtcacggat ttgctgcccg
    28561 ttgccatata ccggcagcgg tttacccgcc agcgcgttga ggatcatcag cgggatcagt
    28621 ttttccggaa agtggtaagg accgtagtta ttcgagcagt tggtgatcag cgtcggcaga
    28681 ccgtaggtcc gcagccaggc gcgcaccaga tggtcgctac tggcttttga cgcggaataa
    28741 gggctgctcg gcgcatacgg cgtggtttcg gtgaagaaat catccgtcga gtgcaggtca
    28801 ccatatactt cgtcggtgga gatatgatga aaacggaacg ctgatttttt atcttccgtc
    28861 agcgtattcc agtaagcccg cgccgcttca agcaatgtat aagtcccgac aatgttggtt
    28921 tcaataaacg ctgccgggcc gtcaatagaa cggtcaacat ggctttcggc tgccagatgc
    28981 atgacacagt ctggctgatg ctcagtgaat acgcgtgcca gttctgcccg atcgcagata
    29041 tcaactttct caaaggcaaa gtgctcgctt tgcgcgaccg gtgccagcga catcaggttt
    29101 ccggcgtagg tcagcttatc gaccactacc accgcatcgc tcgtttcgtt gatgatataa
    29161 cgcaccagcg ccgagccaat aaacccggca ccacctgtta tcagaatttt tctcatcagc
    29221 gccagactcc tttggcatcg acgacatact gctgatggac attgtcgcca ttgataactt
    29281 tgaactgact atgatcgacc agcatcacca gcacgtctgc cgttgccagc gcctcgtcaa
    29341 gctgcgccag agtacaaagc ccggtcagtt ttttcggcaa ctggtggatg ttaggctcaa
    29401 caaccagcgt ttcgccgctg tgccactggg cgatcagttc agcgatttcc atcgccgggc
    29461 tttcgcgcag gtcatcaata ttcggtttaa acgccagacc aaagcaggcg attttcagtt
    29521 cactggcgcg tttatcggta gcagcaaggc agtcggcaac cgccgctttc acctgatcga
    29581 taacccagaa cggtttgtga tcgttaactt cgcgcgcggt acggataagc cgcgcctgct
    29641 gggggttctg tgccacgata aaccacggat caacagcaat gcagtgaccg cccacgccag
    29701 ggccaggctg aagaatatta acgcgagggt gacgattcgc caggcgaatc agttcccaga
    29761 cgttaatccc ctgatcggca caaatcagcg acaattcatt agcaaaagcg atattcacat
    29821 cgcggaagct gttttcggtg agcttacaca tttccgccgt ccgcgagtta gtgacgacac
    29881 actcaccttc gaggaaaatt ttgtacagtt cgctggcgcg ggtcgaacaa accggcgtca
    29941 taccaccaat cacgcgatcg tttttaatca gctcgaccat aacctggccc ggtaacacgc
    30001 gttccgggca gtaagcaatg ttgacgtccg cctgctcgcc cacctgctgt gggaaagtga
    30061 gatccggacg catctctgct aaccattctg ccatcttctc ggttgacccc accggcgagg
    30121 tggattcaag gatcaccagc gcgccttttt tcagcactgg cgcaatggag cgagcagccg
    30181 attcaacgta ggtcatatct ggctcatgat cgcctttaaa cggcgtgggt acagcaatca
    30241 gccaggcatc cgcttcaact ggcgtcgtgc tcgctcgtaa aaaaccgcct tctacggcag
    30301 tttttactac gctcgccaaa tcaggctcga cgatatggat ttcgccacga ttgatggtat
    30361 caaccgcgtg ttggttgata tcgacaccaa tcacctgttt ttgccgtgag gcaaacgctg
    30421 ctgctgtagg cagcccgatg taacccagtc cgataacaga aatggtcgca aaactcatag
    30481 tgatatccga ttatttttta acgcttccag aatgcgagag catgcctgac catcaccata
    30541 cgggttatgg gcgcggctca tagtttgata ttcgttttcg tcttttaaaa gacgcgtcac
    30601 ttcctcgaca attcgctgct tatccgtgcc taccagacgc accgtacccg ccgtcaccgc
    30661 ttccggacgc tcagtggtat cgcgcatcac cagcaccggt ttccccagcg aaggcgcttc
    30721 ttcctgaatg ccgcctgagt cggtcaaaat cagccaggcg tggttcatca gccagacaaa
    30781 cggtaaatac tcctggggat cgatcagaat gacatttttc acatgcccca gaatgcgatt
    30841 gaccggctct ctgacgttcg ggttgagatg caccggatag acaatctgga tgtcctggtg
    30901 cgtggtggcg atgtctgcca gcgcgtggca gatttcttca aagccacgac cgaaactttc
    30961 acgcctgtga ccggtcacca gaatcatctt tttatcgggg tcgataaacg ggtaatttgc
    31021 cgccagttct gaacgcagcg tgtcgctgct catcacctga tcacgcaccc ataacagtgc
    31081 atcaatgact gtattaccag taatgaagat tcggctatcc gcaacgtttt cacgcagcaa
    31141 gttttgccgg gaagtttcgg ttggagagaa gtgatacatc gccagatgcc cggtcaatgt
    31201 acggttagcc tcttccggcc acggcgaata gagatcgccc gtgcgtaaac cagcctcaac
    31261 gtgaccaaca ggaatacgct gataaaacgc cgccaggctg gttgccagcg tcgttgttgt
    31321 gtcgccgtga accagcacga cgtctggttt gaactcggca agaataggtt ttagcccttc
    31381 cagaatccga caggttatct ctgtcaggcc ctgtcctggc tgcattatgt tgagatcgta
    31441 gtcaggtaca atggaaaaga gtttcagcac ctgatcgagc atctcccgat gctgcgcagt
    31501 gacgcaaact ttagcctcaa aaaaaggatc ttttgccaac gcatgcacca acggagccat
    31561 cttgatggct tccgggcgcg taccaaatac agtcagtact ttcacatcga ttctcttcga
    31621 ataagcggcg agcgcctttg cgctcgcagc agcagtgttg ctatttcgag caacggcggg
    31681 ttaatgcgac accagccccg atcagccccc cgacaatgcc ccacataatc atcaggaagg
    31741 cacgacgtgg gctatcgcgt tttaccggtt cttccggcgt acgcaaatag cgataggtct
    31801 gaaaacgcgg atccagagtt ggaccaacat tcagggtgtt taacatggcc cgattctgat
    31861 catagtcgag atcaaaggcc ggaccgacgg cctgtaaatt ttccagtcga gcctggagca
    31921 ttggacgccc aagcaggaac atttctgaat caggtaattc ctcggcaggc acatctgtcg
    31981 cactgcgcga aatattatgc tgctcagcaa ttttcagcgc ctgctcaatg ctgttcatcc
    32041 ggcggtcgta gatggctttc gccacctctt cctgacgctt cacctgagct ttcatctgga
    32101 tggtgcgtgc cgcccatgcg cctttcagct catcattcag atggctggct gcacgctggc
    32161 tggcaaaagc aacatactga cgtaacaggt tattagcgtc aggcgcggtt tcggcaataa
    32221 gcttcacgct gtcattgacc gcgcgggtaa agtctccggg gataaactgg atgttgttaa
    32281 tcatttcatc cagcaacgcc gcatcggctt tgctgttgcc caccatccgc tgtttgtaat
    32341 agtcggtttg cagccagaac tctctgcggg tatcccacga ggccagttgc ataacaaact
    32401 ctttataggc ttcgtccatg accgatggtt gatcggcaga agccatgttt gaacggacat
    32461 ccaggttacg caaaaattgc tgctgcgagt aatatccccc cagcatattc accgttggac
    32521 gatcggtaat cgccgtcgag ctccactcct gacgagcaaa aaaagtatac gccagcgcga
    32581 ttaacgcaaa cgccagcccc atgccaataa tccatagctt cccagcccac aaggtacgaa
    32641 acaacccacg aatatccagt tcattttcag cgtcttcggc cggtttccca ggcattggtt
    32701 gtgtcatcac atcctcattt atttggttaa attggggctg ccaccacgat ttctacgcag
    32761 tctgcgtttt acgcgcttaa taaagcgagc aactttccag gcacgcttaa tgcaatatcc
    32821 atagaggaag aatgctagca aaaagagcac cagcatgacc cactccggga caaaatgaga
    32881 atattctgcc agcacgccaa tggaagcgag cagtgctgcg gcaagggtaa tcagcacaaa
    32941 cgcctgacgg gaagtaaacc cggcacgcat gatcaaatgg tgaatatgct gacggtcagg
    33001 agagaatggg ctcatgcctt tacgcaggcg acggtacata atcgccacca tatccattag
    33061 cggaatggcg attatccaca aagcggtaac cgggctgatg ggatgggttt tgccctgggt
    33121 cgtttcgagc aggatccaga taacggtaaa accaatcagc gtactgcccg catcacccat
    33181 aaagactttg tagcggcgac ccaggatacc aaggttaagc atgatgtatg gcaggatggc
    33241 ggcgatcatc gcaaagcacc agattgcgag gctggtttgc ccgtcgaacc acaaaatcat
    33301 accgattgct gcaaacgaga cgcaggacaa cccgcccagc aagccatcaa tgccatcaac
    33361 catgttgaac gcattaatgg ccgcccagac ggcaaatagc gtcaggaagt aaccaaacgg
    33421 tccgagcacc atctcccagg agccaaagat ataacccagg ctactgagat aaagtttgcc
    33481 gaacaccatc ataacaatgc caacagcggc ctgtatggtg gcacggattt ttacgctgat
    33541 atcaaaacgg tcatccagcg cgccaatgaa aacaagcaca ccggcacaag cgagatagag
    33601 agatgcatgc ggaatatagt aatcgacaat tccgaacgtg aagcaaatcc ctgcgtaaac
    33661 cgaaatcccc ccaacgagag gtatcaatcc ctggtgacgt ttgcggaagt ttggtttatc
    33721 cactaaaccg actttttttg ccactttacg ggcaaaaaac agaaacagtg tcgtgaataa
    33781 aaaaatactg atgagatcag tactcactgt cagtaaattc acaatgcatg ctctcagaga
    33841 aaattattag cagaagtata accacgaaga cctttattca gaagggaaag tccgttccga
    33901 acccaccagt cctgtgcaaa aattaaccat ttgatatgca aattgcataa catgccagca
    33961 aattccagga attttcctaa catcgccagc gcggcattaa gattacagat ataaaagcaa
    34021 aacgccacgt aaacacgtgg cgtttttggc ataagacaaa tttatgagcg tttcatcatt
    34081 tcgaagaaat cgtcattggt cttagtcatg gccagtttat taatgaggaa ttccattgca
    34141 tcgatttcgc ccatcgggtg aatgattttg cgcaggatcc acattttctg cagttcttcc
    34201 tgagtcgtga gcagctcttc tttacgggta ccggaacggt tgtagtcgat agccgggaag
    34261 acgcgttttt cagcgatctt acgagagagg tgcagttcca tgttgcctgt acctttaaac
    34321 tcttcgtaaa taacttcgtc cattttagaa ccggtatcga taagcgccgt cgcgataatg
    34381 gtcaggctgc cgccctcttc cacgttacgc gccgcaccga agaagcgttt cggacgatga
    34441 agggcattgg cgtccacacc accggtcaac actttacctg acgccggaac aacagtgttg
    34501 taagcgcgcg ccagacgagt gatggagtcg agcagaatga taacgtcttt cttgtgctca
    34561 accaggcgtt tcgccttctc gatcaccatt tccgcaacct gaacgtggcg agatgcgggt
    34621 tcgtcaaagg tagaagcaac aacttcacct tttaccagac gctgcatctc ggttacttct
    34681 tccggacgtt cgtcgatcag cagaaccatc agcacacaat ccgggtggtt gtaagcaatg
    34741 ctctgagcaa tgttctgcag cagcatggtt ttaccggctt tcggcggtgc cacaatcaga
    34801 ccacgctgac cacgaccgat aggtgatgcc agatccagta cgcgagcggt taaatcttca
    34861 gtagaaccgt taccacgttc catacgcaga cgagagtttg cgtgcagcgg ggttaagttc
    34921 tcaaagagga ttttgttgcg ggcgttttca ggtttgtcga agttaacttc gttaactttc
    34981 agcagcgcaa aatagcgttc accttctttc ggcgggcgaa tcttaccaga gatggtatca
    35041 ccagtgcgga ggttgaaacg gcggatttgg ctaggggaaa cgtagatgtc atcaggaccg
    35101 gcgaggtagg agctgtctgc ggaacggagg aaaccaaatc catcctgcaa tatctccagt
    35161 acgccatcac caaagatatc ttcgccactc tttgcgtgct gcttcaggat ggcaaaaata
    35221 atgtcctgct tacgcatacg agccaggttt tccagcccca tattttcgcc gagagtgatc
    35281 agctcagaaa ccggcgtatt ctttaattcg gtaagattca tagtggtgtg agttcttaaa
    35341 cttggggtaa atctcgaact taatgttgtg aatggtatgg caggatcatc catgcctgtt
    35401 tacggtcatc gactcatgtc ttttcgctgc ctggtcacag gagagtacgc agaactgaaa
    35461 cgacaagacg gattgagtga cgagccagaa atctgttcac ttcgcattta agataaaacg
    35521 ggaagcgttg gaaattacaa gattcaaact taataaggta tgtttaatac gaagtcaaca
    35581 ctaagttagc atgactcacg ccgggcgtcc agtttttagc gacggggcac ccgaacatga
    35641 aattccctta cgccaggtta gcgtcgagga actctttcaa ctgaccttta gacagtgcgc
    35701 ccactttggt tgccgccact tcaccgtttt tgaacagcag cagagtcggg ataccacgga
    35761 tgccatattt cggcgcagtg ccagggtttt gatcgatgtt cagttttgca acggtcagtt
    35821 tgccctgata ttcgtcagcg atttcatcca gaatcggggc gatcattttg cacggaccgc
    35881 accactctgc ccagaaatcg acgaggatcg ccccgtccgc tttgagtaca tccgtgtcaa
    35941 aactgtcgtc agtcaggtga ataattttat cgctcatata taactccaca ggaataagcc
    36001 tggcgtgttg gtttcgttgt tggtgtaaca ttaaccaact aaaggttgac tttatttcac
    36061 cggatacgct ttcgtaaagc aatagtaagc tgatattcta ccacactatg agcaaaacac
    36121 atttaacaga acagaagttt tccgacttcg ccctgcatcc gaaggttgta gaagcccttg
    36181 aaaaaaaagg gtttcataac tgtacgccca ttcaggcact ggcccttccg ctgacgctgg
    36241 cgggtcgtga cgtagccggg caggcgcaaa ccggtaccgg gaaaacgatg gcgtttctta
    36301 cgtcaacgtt tcattatctt ctctctcatc ctgcgattgc cgatcgcaag gtgaatcaac
    36361 cacgtgcctt aattatggca ccgacgcgtg aacttgccgt gcagatccat gccgacgcag
    36421 aaccgctggc ggaagctacc ggcctgaagc tgggtctggc ttacggtggt gatggctacg
    36481 acaaacagct gaaagtgctg gaaagcggcg ttgacattct gattggcact acggggcgtt
    36541 taattgacta cgccaagcag aaccacatta acctcggtgc cattcaggtg gtggtactgg
    36601 acgaagccga tcgcatgtac gatctgggct ttattaaaga tatccgctgg ctgttccgcc
    36661 gtatgccgcc tgcaaaccag cgcctcaaca tgctgttctc cgccacgctt tcgtaccggg
    36721 tacgtgaact ggcgttcgag cagatgaaca atgccgaata tattgaagtg gaaccggaac
    36781 agaaaacggg ccaccgtatt aaagaagagc ttttctaccc ttctaacgaa gaaaaaatgc
    36841 gtttgctgca aacgctgatc gaagaagagt ggccagacag agcgattatt ttcgccaata
    36901 ccaaacaccg ttgtgaagag atctggggcc acctggcggc agatggtcat cgtgtcggtt
    36961 tgttgacagg cgatgtcgcg cagaaaaaac gtctgcgtat tcttgatgaa tttacccgtg
    37021 gcgatctgga tattctggtt gccaccgacg ttgccgcgcg tggtttgcat attccggcag
    37081 tgacgcacgt ctttaactac gatttacccg atgactgtga agattacgtt caccgtattg
    37141 gtcgtacagg tcgcgcaggc gcaagcggtc actctatcag cctggcgtgt gaagagtatg
    37201 cattgaattt gcctgctatt gagacctata ttggtcactc aattccggta agcaaataca
    37261 atccggacgc attgatgacc gatctgccaa aaccgctgcg cctcacgcgc ccacgtacag
    37321 gcaatggccc gcgtcgtact ggcgctccgc gtaatcgtcg tcgttcaggt taaataaaat
    37381 gtcatactca aaatcattca aactgtatca agatggcaag agaatgaatc ccgatgagca
    37441 tactgtagta agtgattcag gtgaaagaat gcagccaaca cagagacaga ttgaaggatg
    37501 aagagtatgg gttccacctc gtcgctgtat gcagccattg atctcggttc gaatagtttt
    37561 catatgctgg ttgtgcgcga ggtggctgga agcatccaga cgctgacgcg aataaaacgc
    37621 aaagtgcgtc tggctgctgg cctgaatagc gaaaatgccc tgtctaatga agcaatggag
    37681 cgcggttggc aatgtctgcg cctgtttgct gaacgtctgc aagatatccc tccctcgcaa
    37741 attcgcgttg tcgctacggc gacattacgt cttgccgtca atgcgggtga ttttattgcc
    37801 aaagcgcagg aaatcctcgg ttgtccggta caggtgatca gcggtgaaga ggaagcacgt
    37861 ctgatttatc agggcgttgc tcacacaact ggcggtgccg atcagcgcct ggtggtggat
    37921 ataggcggtg ccagtactga actggtaacc ggcacgggtg cacaaaccac ctcgttgttc
    37981 agcctgtcga tgggctgcgt cacctggctg gaacgctatt ttgccgatcg taatctgggg
    38041 caggaaaatt ttgatgctgc agaaaaagcg gcacgcgaag tgttacgtcc ggttgccgat
    38101 gaattacggt atcacggctg gaaagtgtgc gttggtgctt ccggcaccgt gcaggcgtta
    38161 caggaaatca tgatggcaca ggggatggat gaacgcatta ccctggaaaa gttgcagcaa
    38221 ttgaaacagc gagccattca ttgcggtcgg ctggaggaac tggagattga cgggctgacg
    38281 ctggaacgtg cgttagtgtt cccgagtggt ctggcgatcc tgatcgccat ttttaccgaa
    38341 ctgaatattc agtgtatgac cctggcgggc ggtgcgctgc gtgaaggcct ggtctacggc
    38401 atgttgcatc tggccgtcga gcaggatatt cgcagccgta cgctgcgtaa tattcagcgc
    38461 cgctttatga tcgatattga tcaggcgcag cgcgtagcca aagttgcggc taacttcttc
    38521 gatcaggtgg aaaaggaatg gcatcttgaa gcaataagcc gcgatttgct catcagcgcc
    38581 tgtcagcttc atgaaatcgg cctgagcgtt gactttaaac aagcgccgca acacgctgct
    38641 tatctggtgc gtaatctgga tcttcccggt tttacccccg cacagaaaaa attgctggcg
    38701 acgctactgc tcaaccagac taatccggtc gatctctcat cgctgcatca gcaaaatgcc
    38761 gtacctccgc gcgtcgcaga acaactctgc cgtttactcc gcctggccat catttttgcc
    38821 agccgtcgcc gtgacgatct cgtgccagag atgacattac aggctaacca tgaactgttg
    38881 accttgacgc ttccgcaagg ttggctaacc caacatccgc tgggtaaaga gattattgct
    38941 caggaaagcc agtggcagag ctatgtccac tggccgctgg aagtgcatta atgagtaagt
    39001 gccggatgcg atgctgacgc atcttttccg gccttgatta tttccctcgt tttgccgcca
    39061 tcatcgcttt cagattcgcc agatggcttt gccctttctg catccgttct tcggcgctga
    39121 ccactttgcg ctcctgttcc caaatcagat catcctgcgg cagctccagc aaaaagcggc
    39181 tcggctccgg gcgcaccagt tcgccgtact gacggcgttc tttacacagc gtaaaggtca
    39241 attccttctg ggcgcgggta atgccgacat aggccagccg ccgctcctca tcgatattat
    39301 cttcatcgat actgctctgg tgcggcaaaa acccttcttc cataccgacc atgtagacat
    39361 aaggaaactc cagccctttc gacgcgtgga gagtcatcag ttgcacctga tccagctctt
    39421 cttcactctc accacgctcc atcatgtcgc gcaaagtaaa gcgcgtcacc acctgggtga
    39481 gcgtcatcgg ctcatccagt tcgctacctt ccagcatctc cgtcatccag ctaaacagtt
    39541 ggttgacgtt cttcatgcgc atttcggcgg ctttcgggct gggcgatgtt tcgtacagcc
    39601 aggattcata atccatgccg tggatcagat cgcgcaccgc ggcaatcggc tcccgctccg
    39661 ccagacgctg gatttctgcc aaccagtgag taaagcgggt caatgcttca taaccacgtc
    39721 cgctaagcgt ctgactcagg cccatatcaa agctggcggt aaacatgctt ttattgcgcg
    39781 tcatcgccca ctcacccagc tttttcagcg tcgccgggcc aatctctctc ttcggcgtgt
    39841 taacgatacg cagaaatgcg ctgtcatcgt ccgggttagt cagcacgcgc agataagcca
    39901 gcaagtcctt gatttcagga cgagagaaaa acgacgtacc accagatatt ttgtatggga
    39961 tgcggttttg catcaggaat ttttcaaaca cccgcgactg atggttaccg cgataaagaa
    40021 tggcgtaatc tttgtactgc gttttattga cgaagtgatg ggcgatcagc tcgccagtaa
    40081 cgcgctcggc ttcatgttcc tcgttattcg cgcttaaaac ttttagctct gtgccgtaac
    40141 ccagttcgga gaacagacgc ttttcaaaga cgtgcggatt attggcgatc aggatgttcg
    40201 ccgctttcaa aatacgtccg gaagagcggt agttctgctc aagcttaatc accttcagcg
    40261 ccggaaaatc ctggctcagc agcaccaggt tttgcggacg tgcgccgcgc caggagtaga
    40321 tcgactggtc atcgtcaccc accacggtaa agcgcgcgcg gctgcccacc agcagtttca
    40381 ccagttcata ctggctggtg ttggtgtcct gatattcgtc caccagcagg tagcgaattt
    40441 tgttctgcca gcgctcgcgg acttcttcat tgcgttgcag aagtagcgtc ggcaataaaa
    40501 tcagatcatc gaagtcgagc acattacagg ctttcaggtg tgcatcatac agcccataac
    40561 aatgggcaaa aatacggtcc cgctcgccaa tcgcacttgc tgccgcctgg gacggtgttt
    40621 tgagatcatt cttccagtta gagatggtcg aaatcagttg ttgcaggaga actttgtcat
    40681 cttcaatcag cccctcggtc aactctttaa gcaaagcaag ctgatcggta tcgtcaaaca
    40741 gcgaaaagtt cgccttcatc ccaagcgccg catactcgcg tttgatgata tccaacccca
    40801 gcgtgtgaaa agtggagatc atcagcccgc gcgcctcttt gcgacccagc gtctgcccga
    40861 cacgctcttt catctcgcgc gccgccttat tggtaaaggt caccgccgcg atatgtcgcg
    40921 cctgataacc gcaaccgcgg atcagatggg cgattttatt ggtgatcaca cgagttttac
    40981 cggaacccgc gcccgccagc accaggcagg ggccggtaac gaattcgaca gcttgttgtt
    41041 ggccggggtt tagacgcata ggtgtattgc tcaatcttcg aacgggggga ggattgtagc
    41101 agaatcgcat ttgaatgtga ccgcgtgtaa gccgctaacc ggttggctgc ggatgtttaa
    41161 atcagagaga gaagaccgaa accggagttc atcgtgaatc cattagactt agaaaatatc
    41221 gggtcgtaac gttgtgttat tactttttca gagtttacta aggagaggtg tactcgtggc
    41281 gattaatttc agtcctaagg ttggtgaaat actggaatgc aactttggta actatcctgt
    41341 tagccagaat ggacagttca gcaccaagta ttacgatggc cgcattcctc ctgaaatgat
    41401 aaaaaacagg ttagttgtcg tgctcaacgg aaaaattaat ggcaacgcat gtatcgtcgt
    41461 tccattatca accactcgcg atcatgacaa gttaaatcgt ggaatgcatg ttgaaatagc
    41521 cagtaatgtt atcaatggcc tgcaattctt tgatcagcag attcgttggg ccaaagccga
    41581 cctggtacaa caagtcagtc gaaaccgatt aaatagagca aggacttaca gagggtatct
    41641 taatcaatgt ttgccacatg agttggttgc agacatacag agggcggtca tcaagtcaat
    41701 caatgccata tcgctcataa attgacaata aaatcaccat ccccgataat gactttgtag
    41761 cccatagagg ccctaagttt tctccccttg agggacctgc gaagcccgga ttttccgggc
    41821 ttttgccata tatgtaccag cccctccctg actgacaatt acgttagcat ctgcggatca
    41881 tgctaaaatc gccgcctgac aattattcac tcaaggcacg atcatggcaa aaacagcagc
    41941 agcactgcat atccttgtaa aagaagagaa actggctctg gatcttctcg agcagattaa
    42001 gaacggtgcc gatttcggca agctggcgaa gaaacactcc atttgcccat caggcaaacg
    42061 cggcggtgat ttaggtgaat tccgccaggg tcagatggtt ccggcgttcg ataaagttgt
    42121 gttctcttgt ccggtactgg agccaaccgg cccgctgcac acccagttcg gttaccacat
    42181 cattaaggtg ctgtaccgta actaatagca aggccttctc caggagaagg ccttgagtgt
    42241 tttctccctc tccctgtggg agagggtcgg ggtgagggca tcagcgcgca cttaacccgc
    42301 aactgcaata cgtttcatat ccgtcatata gccgcgcagt ttcttaccta cctgctcaat
    42361 cgcatggcag cgaatcgctt cgttcacatc acgcagttgc gcgttatcta ccgcaccttc
    42421 cggaatagct ttacccagat cgcccggttg cagctctgcc ataaacggtt tcagcagcgg
    42481 cacacaagcg taagagaaca gatagttacc gtactcggcg gtatcagaga taaccacgtt
    42541 catttcgtac agacgcttac gggcgatggt gttggcaatc agcggcagtt cgtgcagtga
    42601 ttcatagtaa gcagattctt cgatgatgcc ggaatcaacc atggtttcaa acgccagctc
    42661 aacgcccgct ttcaccatcg cgatcatcag tacgccttta tcgaagtact cctgctcgcc
    42721 gattttgcct tcatactgcg gcgcggtttc gaaggcggtt ttgccggtct cttcacgcca
    42781 ggtcagcagt ttcttatcat cgttggccca gtccgccatc ataccggagg agaattcgcc
    42841 ggagatgatg tcgtccatat gtttctggaa caggggtgcc atgatctctt tcagctgttc
    42901 agacagcgcg taagcacgca gtttcgccgg gttagagaga cggtccatca tcagggtgat
    42961 gccgccctgc ttcagcgctt cggtgatggt ttcccaaccg aactgaatta gtttttctgc
    43021 gtatgccgga tcggtgccct cttccaccag cttgtcgaag cacagcagcg agccagcctg
    43081 caacataccg cacaggatgg tttgctcacc catcaggtca gatttcactt ccgcaacgaa
    43141 ggacgattcc agcacgcccg cacggtgacc accggttgct gccgcccatg ctttagcgat
    43201 cgccatacct tcgcctttcg gatcgttttc cgggtgaacg gcaatcagcg tcggtacgcc
    43261 gaatccacgt ttgtactctt cacgcacttc ggtgccaggg catttcggcg caaccatgac
    43321 tacggtgata tctttacgga tctgctcgcc cacttcgacg atgttgaaac cgtgcgagta
    43381 acccagcgcc gcgccgtctt tcatcagtgg ctgtacggtg cgcactacat cagagtgctg
    43441 cttgtccggc gtcaggttaa ccaccagatc cgcctgcggg atcagttctt cgtaagtacc
    43501 aactttaaaa ccgttttcgg tcgctttacg ccaggacgcg cgcttctcgg caatcgcttc
    43561 tttacgcaga gcgtaggaga tatcgagacc agaatcacgc atgttcaggc cctggttcag
    43621 cccctgtgcg ccacagccga cgataaccac ttttttaccc tgaaggtagc tcgcgccgtc
    43681 ggcgaattcg tcgcgcccca taaagcgaca tttgcccagc tgtgccagct gctggcgcag
    43741 gttcagtgta ttgaagtagt tagccatggt gattcctcgt gatgttgtgc ttcttattgt
    43801 ttggttcgcg tttgcgagat tgaattcact atatgacagg aaatttattg cggaaattga
    43861 tatattcaca acgtcacatt gcaatttctg caacgtcaac attgagggct gcgccattgg
    43921 atttacgcga tctgaaaacc ttcctgcatc tggcggaaag ccgccatttt ggccgcagcg
    43981 cgcgggcgat gcacgttagc ccgtccacgc tctcacggca gattcagcgc ctggaagacg
    44041 atctcggtca ggcattattt gtgcgcgata accggacggt gacgctgacc gaagcgggcg
    44101 aagagttacg cgttttcgcc cagcaaacat tgttgcagta tcagcaactg cgccacacca
    44161 tcgatcagca agggccgtcg ctctctggcg agttacatat tttctgctcg gtgaccgccg
    44221 cttacagcca tctaccgccg attctcgacc gcttccgcgc ggaacacccg tcggtggaga
    44281 ttaaacttac tactggtgat gcggcagatg cgatggaaaa ggtggtcact ggtgaagcgg
    44341 atctggcgat tgcgggtaaa ccggaaacct tgcccggcgc agtggcgttt tcgatgctgg
    44401 agaatctggc agtagtgctg attgcccccg cgctgccctg cccggtgcgt aatcaggtgt
    44461 cggtagagaa gccggactgg tcaacggtgc cgtttatcat ggccgatcag gggccggtac
    44521 gccgccgcat tgaactgtgg tttcggcgta acaaaatcag taacccgatg atttacgcca
    44581 cggttggcgg gcatgaagcg atggtatcga tggtggcgct cggctgtggc gtggcgctgt
    44641 tgccggaagt ggtgctggaa aacagcccgg aaccggtgcg taaccgcgtg atgatactag
    44701 agcgaagcga tgagaaaacg ccgtttgagc tgggcgtgtg tgcgcagaaa aagcggctgc
    44761 atgagccgct gattgaagcg ttctggaaga ttttaccgaa ccactaaccc gccaaaaaga
    44821 acctgaacgc cgggttattg gtttcgtcgt ggcaatcgta gcccagttca ttcagccggg
    44881 tttcgaaatc cggttcatgg tcgccaagct cgaacgccgc cagtacgcgc ccgtagtcgg
    44941 taccgtgact gcgataatgg aacagagaaa tattccagtg cgtacccagc gtgttgagga
    45001 agcgcagcag cgcacccggc gattccggaa attcgaagct gtagagacgt tcctgcaacg
    45061 gatgcgatgg acgcccgcca accatatagc gcacatgtag cttcgccatt tcatcgtcgg
    45121 agagatcaac cacgctgtag ccgccgtcgt tgagcatctg caaaatttct ttgcgctctt
    45181 cgaggccgcg gcttaagcgc acgccgacaa agatgcaggc gtttttggca tcggcaaagc
    45241 ggtagttgaa ttcggtgacc gaacgtccgc caagcaactg gcagaatttg aggaagctac
    45301 ctttctcttc cggaatggtt accgccagca acgcttcacg ctgttcaccc agctcacaac
    45361 gttccgagac gtagcgcagg ccgtgaaagt tcacgttagc accggaaaga atatgcgcca
    45421 gccgttcgcc gcgaatgttg tgctgggcga tatatttttt cattcccgcc agcgccagcg
    45481 cgccagaggg ttccgccacc gcacgcacat cttcgaataa atccttcatc gccgcacaga
    45541 tcgcatcgct atcgacggtg atgatgtcgt cgagatactc ctggcataaa cggaaggttt
    45601 cgtcaccgat gcgttttacc gctacgcctt cagcaaatag ccctacgcgc ggcagatcaa
    45661 ccggatgacc cgcatccagc gctgctttca ggcaggcgga gtcttccgct tctacggcga
    45721 tcactttgat ttgtggcatc agttgtttga tcagcaccgc aacgcccgcc gccagaccgc
    45781 cgccgccgac tggcacaaat acgcggtcga gatgggcgtc ctgctggagc agttccagcg
    45841 ccagcgtgcc ttgcccggca atcaccatcg gatggtcgaa cggcggcacc caggtgaacc
    45901 cctgctgctg tgacagttcg atcgctttag ctttcgcttc gtcaaagttc gcgccatgaa
    45961 gcagcacttc gccgccgaat ccacgtaccg catcgacttt gatgtcggcg gtggcggttg
    46021 gcataacgat cagggccttc acgcctaacc gcgcagaaga aaacgcgacg ccctgcgcgt
    46081 ggttacccgc agaagcagtg atcacgccgt gcgctttttg ttcttccgtc aggcccgcca
    46141 tcatggcgta tgcgccgcgc agcttaaagc tgtgcactgg ctggcgatct tcgcgcttca
    46201 ccagaatgac gttatcaaga cgcgacgaca gtttttccat tttttgtagc ggcgtaacct
    46261 gcgccgcctc gtaaaccggc gcgcgcagca ctgctcttaa atattcggca ccttccggag
    46321 caccggacag gggttgcgag tcagccatta ttaacccccc agtttcgatt tatcgcgcac
    46381 cgcacctttg tcggcgctgg ttgccaggct ggcgtaggcg cgcagggcaa aggaaacctg
    46441 acgttcacgg tttttcggcg tccaggcttt gtcaccccga gcttcctgcg cttcacgacg
    46501 cgccgccagt tcggcatcgc ttacctgtaa ctgaatgcca cggttcggga tgtcgatagc
    46561 gatcaggtca ccatcttcaa tcaggccaat gctgccgccg tttgccgctt ccggtgagac
    46621 gtggccgatg gaaagaccag aggtgccgcc agagaaacga ccgtcggtga tcagcgcaca
    46681 ggctttaccg agacccattg atttcaggaa gctggttggg tagagcattt cctgcatgcc
    46741 agggccgcct ttcgggcctt catagcggat caccaccaca tcgcccgcga caactttgcc
    46801 accgaggatc gcttctaccg cgtcgtcctg gctttcgtac actttcgccg ggccggtgaa
    46861 tttgaggatg ctgtcatcga cgcccgcggt tttaacgatg cagccgtttt ctgcgaaatt
    46921 accgtagagc accgccaggc cgccgtcttt gctgtaggcg tgttccagcg agcggataca
    46981 gccattggcg cgatcgtcat cgagagaatc ccaacggcaa tcctgcgaga atgcctgtgt
    47041 ggtacgaatg cctgcaggac ctgcgcggaa catatttttt accgcgtcat cctgggtcag
    47101 cataacgtcg tattgttcca gcgtttgcgg caacgtcagg ccaagtacgt ttttcacatc
    47161 acggttcagt aaccccgcgc gatccagttc gccgagaata ccgataacac caccagcacg
    47221 gtgaacatct tccatatggt atttctgggt gctcggcgca actttacaca gctgtggaac
    47281 cttgcgggaa agcttatcga tatcactcat ggtgaagtcg atttccgctt cctgcgccgc
    47341 cgccagcagg tgaagtacgg tgttagtcga tccacccatc gcgatatcca gcgtcatggc
    47401 gttttcaaac gccgctttgc tagcaatatt gcgcggcagt gcactttcgt cgttttgctc
    47461 gtagtaacgt ttggtcaatt caacaatgcg tttaccggca ttgaggaaca actgcttacg
    47521 gtccgcgtgg gttgccagca gcgagccgtt gcccggctgc gacaggccca gcgcttcggt
    47581 caggcagttc attgagttag cggtaaacat cccggagcag gagccgcagg tcggacacgc
    47641 ggaacgttca acctgatcgc tctgggagtc agaaactttc gggtctgcgc cctggatcat
    47701 cgcatcaacc agatcgagtt tgattatctg atcggacagt ttggttttcc cggcctccat
    47761 cgggccgccg gaaacaaaga tcaccggaat attcaggcgc agggaagcca tcagcatccc
    47821 cggggtgatt ttgtcgcagt tagagatgca gaccatggcg tcggcgcagt gggcgttgac
    47881 catatactca acggaatcag cgatcagttc gcgagatggc agtgaataaa gcatcccccc
    47941 gtggcccatg gcaatcccat catccaccgc aatggtgttg aactctttgg caacgccgcc
    48001 agccgcttca atttgttcgg cgaccagttt accgagatcg cgcagatgga cgtgacccgg
    48061 tacaaattgg gtgaacgagt tcacaaccgc gataatcggc ttgccaaaat cggcgtcggt
    48121 cattccggtg gcgcgccaca gcgcacgagc acccgccata ttacgaccat gagtggtggt
    48181 ggcggaacgg tacttaggca tactttattt actcccagtg tctgtctcgt aaatgggacg
    48241 gtgcgtgccg tcccattttt tgtatttatt gattaacttg atctaaccag ccccatttat
    48301 cttcggtttc gccagtgaaa aggccgaaga aggcttgctg aatgcgtttg gtaaccgggc
    48361 cacaacggcc ttcgccaacc tgaataccgt ctacgctgcg cactggcgtg atttctgccg
    48421 cagtaccgga cataaacact tcatccgcca ggtacaggga ttcgcgcgac agaacctgct
    48481 cacgcacttc aattcccagc tctttcgcca gtttgatgat ggcatcacgg gtaatacccg
    48541 gcagcgcgga ggaggtgaac ggtggggtga acagcacacc atctttcact tcaaacaggt
    48601 tttcgcctgc gccttcagag atataaccgt tcacatccag cgcgatacct tcctgataac
    48661 cgtggcggcg cgcttcgcta cccaccagca gggaagagag atagttacca ccggcttttg
    48721 ccgcggttgg gatggtgttt ggtgctgcgc ggttccagga ggaaaccatc gcatcgatcc
    48781 cctgctccag cgcttctgcg cccagatacg ctccccacgg gaaagcggcg ataatcacat
    48841 cggttgagta tcccgctggc gggtttacac ccatgccaac atcaccaacg aagatcagcg
    48901 gacggatata ggcgctggtg agattgtttt tgcggatcac gtcacgacaa gcttccatca
    48961 gctcatcaat gctctgcgaa accgggaagc gatagatttt ggcggagtca tgcagacgct
    49021 gcatatgctc acgatggcgg aatacaaccg gtcctttgtg cgagtcgtag caacggatgc
    49081 cttcaaaaac cgaagtgcca tagtgcagcg cgtgcgacat cacatgcacc ttcgcgtctt
    49141 cccagcgaac catctcccca ttgaaccaaa tgtaatcagc tttcttcgtg gtcattttta
    49201 tattcctttt gcgctcaggc gcggatttgt tgtgatgtgg ttgtgctctg gcagatggca
    49261 acgtgtgcga cgtccaccag tttatttaac tgactaaaca gtaagtcgac cgaccgtggg
    49321 ctggcaacgg tcaattcgat atttatattt tgtgcatcgc tggcggcagc catattcatt
    49381 gagcagacgt ggaaaccacg atgacgcacc acgcgtaaaa cacgttctaa ggtttccgga
    49441 ttgaagcgag ccgatacatt gacctgatgt tgcatcatga taatttctcc aacatttctg
    49501 aattactggc gccaggcggc accagcggcc agacgttctc aagttcgtcg attgagacat
    49561 gaagcaggta tggcccatca ctgttcagca tggtgtcgag tgccgcttca acctggtctt
    49621 tacgggtgat gtgttggcca gggatgccga aggcgctggc taacatgagg aaatcggggt
    49681 tatcagtaag ggtggtttcg ctgtatcgtt cctgaaaaaa cagttgctgc cattgtcgaa
    49741 ccatccctaa ccgttggtta tcgagtaaga cgattttcaa cggtaactgc ttgcgtttta
    49801 cggtgcccag ctcttgcaca ttcatcatga aagagccgtc accggagata cagacgacag
    49861 tatcgttcgg tcgtgcgact tgtgcgccaa ccgccgctgg taaaccgaaa cccatggtgc
    49921 ctaagccgct ggaggtaatg aaattttccg ggcgagtgtg tgcgatgtgc tgcgcggccc
    49981 acatctggtg ctgccccaca tctgtggtca cgacgcaatc cgcaggttta cgatccgaca
    50041 gttgttttaa caacaacggc gcgtagatag cgtcaccggg atggtcgtaa cgccaggtat
    50101 gttcatcacg cagctgcgcg cagtattgct gccagtcatt gatatttaac ggctgctgta
    50161 atgctggtaa cagagcattt aaatcacctt gtaatgccac atgtgcctga cgcagcttgt
    50221 tcatttctgc cgggtcgata tccatatgga taacactggc gtgtggtgcg aaggtgttca
    50281 gtttgccggt cacccggtca tcaaaacgtg cacccacggc gatcagcaag tcgcactcct
    50341 gcaccgcgaa gttcgccgct ttggtgccat gcattcccag catgcccagg tagtacggat
    50401 aatctgcttc aactgcgccc agccctttca gcgtgcaggt ggcaggcatt tttgtggtag
    50461 cgagaaattc tcgtaaagca ggaactgcct gcgccatacc cacgccacca ccaacgtaca
    50521 gcatcggttt ttgcgctttt gccagcatct ggcgcgcttg ctcaacttcg gcatggggga
    50581 aagtcacttc gttttcaacg gtggtgaacc acggttccag atcgccgctg gctaactgga
    50641 tatcttttgg gatatcgacc agaaccggac caggacgacc tgagctggca acgtcgaatg
    50701 ctgcagccat gatgcgcggc aactcttcca gcgactgcac cagaaagctg tgcttggtac
    50761 aggctaacga caatcccagg acatccactt cctgaaatgc gtcggtgccg ataaacggtg
    50821 cggacacttg accggtgatg gcaacgacag ggatggaatc taacagtgcg tccgcaagcc
    50881 cggttatcag gttggttgcg cccggaccag acgtggcgat acatacgcca gttttaccgg
    50941 tagcacgggc ataaccgata gccgccattg ccgcaccctg ctcatgtcgg cacagcaagt
    51001 gctccacgcc gccgtcatac aatgcatcgt aaaccggcat aattgcgcca cccggataac
    51061 cgaaaacggt gttcacaccc tgtgcccgca acgcatgtac cacccactgt gcgccattca
    51121 tagttagttc cccgtcctga atcttgagaa acagaatttt gtgctgttat tcattgtctg
    51181 ctcctcggtt atgtttttaa ggtcaaaaaa aacccccgga cctttcggtg cgggggtctt
    51241 agttcgttaa ggcttgattt ctaagccttt cctcgtccaa gtgcagcccc gcacggtggg
    51301 ataataatca ccaccacgct aatcacgacc aggctaatca ctcgtagaag ggctgtcatt
    51361 ttgtcttttc ttgcatcttg ttcgaaggaa tgcctaaaga gttaccatag gttttgtaaa
    51421 tagtacaaga tattttttgg ccgattttta tgatgcgttt aataatttca atataaattg
    51481 ttgttttttt aaaaataaaa tagtgagtga aaaaaattca atttcagtat gaatgttgtt
    51541 cccttcctcg tagttcatcc tttgtccgct gcatttttgc gagcatcatt ccaccgccag
    51601 aacaactcaa cttcacttgc acaaataaac aggaatcagc cactaaaatt cacgaattag
    51661 cgggctacag cagcgatatc ttatgcacat aatggtgact caaggagggc ttatgtcact
    51721 gtcaattgtt catacccgcg cagccctggg agtaaatgcg cccccgatca ctgttgaggt
    51781 acatatcagt aaaggtctac ccggcttaac gatggtgggc ttaccagaaa caacggtaaa
    51841 agaagctcgc gatcgcgtgc gcagcgccat tatcaatagc ggatatgaat atccggcgaa
    51901 aaaaatcacc atcaacctgg ctccagccga tctgccaaaa gaagggggac gatatgattt
    51961 acctatcgcc attgcgttgc tggcggcctc agaacagctt acagccaata agttagatga
    52021 atatgaatta gtcggagaac tggctcttac aggcgctctg cgtggcgttc ccggtgcaat
    52081 ctccagtgca actgaagcta ttaagtcggg cagaaaaatt atcgtcgcga aagataacga
    52141 agatgaagtg gggctaatta acggtgaagg atgcctgata gccgatcatc tgcaagctgt
    52201 ctgtgcgttt ctggaaggta agcacgctct cgaacgcccg aaaccaactg atgcagtatc
    52261 ccgggcgcta caacatgatc tcagtgatgt tgtcggtcag gaacaaggaa agcgaggact
    52321 ggaaattacc gccgctggcg ggcacaacct tttactgatt gggccgccgg gaacaggtaa
    52381 aacaatgctc gccagccgta ttaatggcct gttgccagat ttaagcaatg aagaggcact
    52441 ggagagcgct gcgatattaa gtctggtaaa tgctgaatca gtacaaaaac aatggcggca
    52501 gcgcccgttc cgctcacctc atcacagtgc gtcgttaact gcgatggtgg gcggtggtgc
    52561 aattccaggg ccaggtgaaa tttcgttggc gcataacggc gtgctttttc ttgatgagct
    52621 acctgaattt gaacggcgta cactggatgc cttgcgagag ccgattgaat ccgggcagat
    52681 ccatctttca cgcacacgag caaaaataac ctatccagcc cgtttccagc ttgttgcggc
    52741 gatgaatccc agccctaccg gacattatca gggaaaccat aaccgctgca cgccagaaca
    52801 gacattacgt tatctcaacc ggctctcggg gccctttctc gaccgcttcg atctctcact
    52861 ggagatccca ttaccacccc ccggcatttt gagtaaaacg gtagtgccgg gagaaagcag
    52921 cgccaccgtt aaacaacgcg taatggccgc cagagagcgc caatttaagc ggcagaataa
    52981 actgaatgcc tggctggata gtccggaaat acgccaattc tgcaaacttg agagcgaaga
    53041 tgcgatgtgg ttggaaggaa cactgatcca tctggggtta tcgattcgtg cctggcagcg
    53101 gttattgaaa gttgcacgaa ccattgctga tattgatcag tctgacatta tcacacgtca
    53161 gcatttgcag gaggcagtta gctatcgtgc gattgaccgt ttgctcatcc atctgcaaaa
    53221 actactgaca taaaaaaagg gcatttcgcc ctttttatta atcatcagaa tcggtgtagt
    53281 cttcagcacc ttcaacctgc ggtttaccgc cggaaagggt gtgaaaacgt tttggacgct
    53341 taatacgcgt catatacttg gaccacacgc gttctgcttc tgtcactggc tcacgttcgc
    53401 cacggcatac tgctacgaag agtttctctt cctcggtaac cggctcgcgt ttgccaagat
    53461 ccaactcatt gaaggcataa ccatgacgct caagcagttg tgcctctttg atggtgaaat
    53521 caccatgacg agagaatcca cgtggataat gtttattgtc gaaatatcga ttagtcgtcg
    53581 taaagctttc cgccatcctg cacgctccta attctttgac cgagctagtt atggcgcgga
    53641 gtattagtta cgcttgacag agtgtaaaac aaaacattta aatcataacg acaaataatt
    53701 ttgcggagag cactgtggat acggaattgt taaaaacttt cctggaagtt agccgaacgc
    53761 gtcactttgg tcgagcggct gaatcgctct atctgaccca gtcagcagtg agctttcgaa
    53821 tcagacaact ggaaaatcaa ctgggtgtga accttttcac ccgccacaga aacaatatcc
    53881 gtttaaccgc tgccggtgaa aaactactgc cttatgcaga aacgctcatg agcacctggc
    53941 aggccgcccg taaggaggtg gcgcatacct cacgacataa cgagttttct atcggtgcca
    54001 gcgcctcgtt gtgggaatgt atgcttaatc agtggctggg acgcttgtat caaaatcagg
    54061 atgcccatac aggcttacag ttcgaagcgc gaattgccca acggcagtct ctggtaaaac
    54121 agctgcatga acgccagctt gatcttctta ttaccactga agcgcccaaa atggacgaat
    54181 ttagtagtca gttgctggga tatttcactt tagcgcttta taccagtgcc ccttcaaaac
    54241 taaagggaga tcttaattat ctgcgacttg agtgggggcc agattttcaa cagcatgagg
    54301 caggtttgat cggtgctgac gaagtgccca ttctgacaac cagttctgct gaactggcac
    54361 agcaacagat tgcgatgctt aatggttgca cctggctacc cgtcagctgg gcgcgtaaaa
    54421 aaggcggcct gcataccgtt gtcgatagca caacactttc acggccgctt tatgccatat
    54481 ggctgcaaaa tagcgataaa aatacgttga ttcgcgatct tttgaaaatt aacgtgctgg
    54541 atgaagtgta ttaatatgaa tggctggcaa ggatgccggt agaaggattt acttcggaga
    54601 gggttatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggcaggggcg
    54661 gagagactcg aactcccaac acccggtttt ggagaccggt gctctaccaa ttgaactacg
    54721 cccctaatta g
//
LOCUS       DAZGAN020000019        54596 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGAN020000019 DAZGAN020000000
VERSION     DAZGAN020000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 54596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.019"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            338..424
                     /gene="leuL"
                     /locus_tag="ACHKYO_RS06665"
                     /old_locus_tag="pgaptmp_001333"
     CDS             338..424
                     /gene="leuL"
                     /locus_tag="ACHKYO_RS06665"
                     /old_locus_tag="pgaptmp_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HGU4428204.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            517..2088
                     /gene="leuA"
                     /locus_tag="ACHKYO_RS06670"
                     /old_locus_tag="pgaptmp_001334"
     CDS             517..2088
                     /gene="leuA"
                     /locus_tag="ACHKYO_RS06670"
                     /old_locus_tag="pgaptmp_001334"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HGU4428205.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2088..3179
                     /gene="leuB"
                     /locus_tag="ACHKYO_RS06675"
                     /old_locus_tag="pgaptmp_001335"
     CDS             2088..3179
                     /gene="leuB"
                     /locus_tag="ACHKYO_RS06675"
                     /old_locus_tag="pgaptmp_001335"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGU4428206.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3182..4582
                     /gene="leuC"
                     /locus_tag="ACHKYO_RS06680"
                     /old_locus_tag="pgaptmp_001336"
     CDS             3182..4582
                     /gene="leuC"
                     /locus_tag="ACHKYO_RS06680"
                     /old_locus_tag="pgaptmp_001336"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HGU4428207.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4593..5198
                     /gene="leuD"
                     /locus_tag="ACHKYO_RS06685"
                     /old_locus_tag="pgaptmp_001337"
     CDS             4593..5198
                     /gene="leuD"
                     /locus_tag="ACHKYO_RS06685"
                     /old_locus_tag="pgaptmp_001337"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGU4428208.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5247..6425)
                     /gene="setA"
                     /locus_tag="ACHKYO_RS06690"
                     /old_locus_tag="pgaptmp_001338"
     CDS             complement(5247..6425)
                     /gene="setA"
                     /locus_tag="ACHKYO_RS06690"
                     /old_locus_tag="pgaptmp_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HGU4428209.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6527..6658)
                     /gene="sgrT"
                     /locus_tag="ACHKYO_RS06695"
                     /old_locus_tag="pgaptmp_001339"
     CDS             complement(6527..6658)
                     /gene="sgrT"
                     /locus_tag="ACHKYO_RS06695"
                     /old_locus_tag="pgaptmp_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HGU4428210.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6747..8402
                     /gene="sgrR"
                     /locus_tag="ACHKYO_RS06700"
                     /old_locus_tag="pgaptmp_001340"
     CDS             6747..8402
                     /gene="sgrR"
                     /locus_tag="ACHKYO_RS06700"
                     /old_locus_tag="pgaptmp_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HGU4428211.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8436..8535
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8566..9549
                     /gene="thiB"
                     /locus_tag="ACHKYO_RS06705"
                     /old_locus_tag="pgaptmp_001341"
     CDS             8566..9549
                     /gene="thiB"
                     /locus_tag="ACHKYO_RS06705"
                     /old_locus_tag="pgaptmp_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HGU4428212.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9525..11135
                     /gene="thiP"
                     /locus_tag="ACHKYO_RS06710"
                     /old_locus_tag="pgaptmp_001342"
     CDS             9525..11135
                     /gene="thiP"
                     /locus_tag="ACHKYO_RS06710"
                     /old_locus_tag="pgaptmp_001342"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HGU4428213.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11119..11817
                     /gene="thiQ"
                     /locus_tag="ACHKYO_RS06715"
                     /old_locus_tag="pgaptmp_001343"
     CDS             11119..11817
                     /gene="thiQ"
                     /locus_tag="ACHKYO_RS06715"
                     /old_locus_tag="pgaptmp_001343"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HGU4428214.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11931..12695)
                     /gene="yabI"
                     /locus_tag="ACHKYO_RS06720"
                     /old_locus_tag="pgaptmp_001344"
     CDS             complement(11931..12695)
                     /gene="yabI"
                     /locus_tag="ACHKYO_RS06720"
                     /old_locus_tag="pgaptmp_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4428215.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12781..13659)
                     /gene="araC"
                     /locus_tag="ACHKYO_RS06725"
                     /old_locus_tag="pgaptmp_001345"
     CDS             complement(12781..13659)
                     /gene="araC"
                     /locus_tag="ACHKYO_RS06725"
                     /old_locus_tag="pgaptmp_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HGU4428216.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13998..15698
                     /gene="araB"
                     /locus_tag="ACHKYO_RS06730"
                     /old_locus_tag="pgaptmp_001346"
     CDS             13998..15698
                     /gene="araB"
                     /locus_tag="ACHKYO_RS06730"
                     /old_locus_tag="pgaptmp_001346"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HGU4428217.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDANGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15709..17211
                     /gene="araA"
                     /locus_tag="ACHKYO_RS06735"
                     /old_locus_tag="pgaptmp_001347"
     CDS             15709..17211
                     /gene="araA"
                     /locus_tag="ACHKYO_RS06735"
                     /old_locus_tag="pgaptmp_001347"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HGU4428218.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17380..18075
                     /gene="araD"
                     /locus_tag="ACHKYO_RS06740"
                     /old_locus_tag="pgaptmp_001348"
     CDS             17380..18075
                     /gene="araD"
                     /locus_tag="ACHKYO_RS06740"
                     /old_locus_tag="pgaptmp_001348"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGU4428219.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18150..20501
                     /gene="polB"
                     /locus_tag="ACHKYO_RS06745"
                     /old_locus_tag="pgaptmp_001349"
     CDS             18150..20501
                     /gene="polB"
                     /locus_tag="ACHKYO_RS06745"
                     /old_locus_tag="pgaptmp_001349"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HGU4428220.1"
                     /translation="MAQAGFILTRRWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20666..23572
                     /gene="rapA"
                     /locus_tag="ACHKYO_RS06750"
                     /old_locus_tag="pgaptmp_001350"
     CDS             20666..23572
                     /gene="rapA"
                     /locus_tag="ACHKYO_RS06750"
                     /old_locus_tag="pgaptmp_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HGU4428221.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23584..24243
                     /gene="rluA"
                     /locus_tag="ACHKYO_RS06755"
                     /old_locus_tag="pgaptmp_001351"
     CDS             23584..24243
                     /gene="rluA"
                     /locus_tag="ACHKYO_RS06755"
                     /old_locus_tag="pgaptmp_001351"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HGU4428222.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24360..25175)
                     /gene="djlA"
                     /locus_tag="ACHKYO_RS06760"
                     /old_locus_tag="pgaptmp_001352"
     CDS             complement(24360..25175)
                     /gene="djlA"
                     /locus_tag="ACHKYO_RS06760"
                     /old_locus_tag="pgaptmp_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HGU4428223.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25430..27784
                     /gene="lptD"
                     /locus_tag="ACHKYO_RS06765"
                     /old_locus_tag="pgaptmp_001353"
     CDS             25430..27784
                     /gene="lptD"
                     /locus_tag="ACHKYO_RS06765"
                     /old_locus_tag="pgaptmp_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HGU4428224.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27837..29123
                     /gene="surA"
                     /locus_tag="ACHKYO_RS06770"
                     /old_locus_tag="pgaptmp_001354"
     CDS             27837..29123
                     /gene="surA"
                     /locus_tag="ACHKYO_RS06770"
                     /old_locus_tag="pgaptmp_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HGU4428225.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29123..30112
                     /gene="pdxA"
                     /locus_tag="ACHKYO_RS06775"
                     /old_locus_tag="pgaptmp_001355"
     CDS             29123..30112
                     /gene="pdxA"
                     /locus_tag="ACHKYO_RS06775"
                     /old_locus_tag="pgaptmp_001355"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HGU4428226.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADTTLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLGVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            30109..30930
                     /gene="rsmA"
                     /locus_tag="ACHKYO_RS06780"
                     /old_locus_tag="pgaptmp_001356"
     CDS             30109..30930
                     /gene="rsmA"
                     /locus_tag="ACHKYO_RS06780"
                     /old_locus_tag="pgaptmp_001356"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGU4428227.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30933..31310
                     /gene="apaG"
                     /locus_tag="ACHKYO_RS06785"
                     /old_locus_tag="pgaptmp_001357"
     CDS             30933..31310
                     /gene="apaG"
                     /locus_tag="ACHKYO_RS06785"
                     /old_locus_tag="pgaptmp_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HGU4428228.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31317..32165
                     /gene="apaH"
                     /locus_tag="ACHKYO_RS06790"
                     /old_locus_tag="pgaptmp_001358"
     CDS             31317..32165
                     /gene="apaH"
                     /locus_tag="ACHKYO_RS06790"
                     /old_locus_tag="pgaptmp_001358"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HGU4428229.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32190..32789
                     /locus_tag="ACHKYO_RS06795"
                     /old_locus_tag="pgaptmp_001359"
     CDS             32190..32789
                     /locus_tag="ACHKYO_RS06795"
                     /old_locus_tag="pgaptmp_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HGU4428230.1"
                     /translation="MADYFEIRADYNQHTITVYQAYNDAIADVAVRDGRFGASFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32825..33292
                     /locus_tag="ACHKYO_RS06800"
                     /old_locus_tag="pgaptmp_001360"
     CDS             32825..33292
                     /locus_tag="ACHKYO_RS06800"
                     /old_locus_tag="pgaptmp_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4428231.1"
                     /translation="MRQINSGLNTREIILAMIDEADEPPFAEQRTWHRRRIAFLCDCI
                     SVAIDYGFFCSVSQQSIMNITRMAEGVWITESKTTQLIDIWQQLKQLYPPETIPYVHK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADIIQLRCDV"
     gene            complement(33391..33570)
                     /locus_tag="ACHKYO_RS06805"
                     /old_locus_tag="pgaptmp_001361"
     CDS             complement(33391..33570)
                     /locus_tag="ACHKYO_RS06805"
                     /old_locus_tag="pgaptmp_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HGU4428232.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(33591..34070)
                     /gene="folA"
                     /locus_tag="ACHKYO_RS06810"
                     /old_locus_tag="pgaptmp_001362"
     CDS             complement(33591..34070)
                     /gene="folA"
                     /locus_tag="ACHKYO_RS06810"
                     /old_locus_tag="pgaptmp_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HGU4428233.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34263..36125)
                     /gene="kefC"
                     /locus_tag="ACHKYO_RS06815"
                     /old_locus_tag="pgaptmp_001363"
     CDS             complement(34263..36125)
                     /gene="kefC"
                     /locus_tag="ACHKYO_RS06815"
                     /old_locus_tag="pgaptmp_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HGU4428234.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAVTPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36118..36648)
                     /gene="kefF"
                     /locus_tag="ACHKYO_RS06820"
                     /old_locus_tag="pgaptmp_001364"
     CDS             complement(36118..36648)
                     /gene="kefF"
                     /locus_tag="ACHKYO_RS06820"
                     /old_locus_tag="pgaptmp_001364"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HGU4428235.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36756..38087)
                     /gene="yaaU"
                     /locus_tag="ACHKYO_RS06825"
                     /old_locus_tag="pgaptmp_001365"
     CDS             complement(36756..38087)
                     /gene="yaaU"
                     /locus_tag="ACHKYO_RS06825"
                     /old_locus_tag="pgaptmp_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4428236.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38145..38432)
                     /gene="fixX"
                     /locus_tag="ACHKYO_RS06830"
                     /old_locus_tag="pgaptmp_001366"
     CDS             complement(38145..38432)
                     /gene="fixX"
                     /locus_tag="ACHKYO_RS06830"
                     /old_locus_tag="pgaptmp_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HGU4428237.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38429..39715)
                     /gene="fixC"
                     /locus_tag="ACHKYO_RS06835"
                     /old_locus_tag="pgaptmp_001367"
     CDS             complement(38429..39715)
                     /gene="fixC"
                     /locus_tag="ACHKYO_RS06835"
                     /old_locus_tag="pgaptmp_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4428238.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39766..40707)
                     /gene="fixB"
                     /locus_tag="ACHKYO_RS06840"
                     /old_locus_tag="pgaptmp_001368"
     CDS             complement(39766..40707)
                     /gene="fixB"
                     /locus_tag="ACHKYO_RS06840"
                     /old_locus_tag="pgaptmp_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU4428239.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDTSTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40722..41492)
                     /gene="fixA"
                     /locus_tag="ACHKYO_RS06845"
                     /old_locus_tag="pgaptmp_001369"
     CDS             complement(40722..41492)
                     /gene="fixA"
                     /locus_tag="ACHKYO_RS06845"
                     /old_locus_tag="pgaptmp_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HGU4428240.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGSDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            41966..43480
                     /gene="caiT"
                     /locus_tag="ACHKYO_RS06850"
                     /old_locus_tag="pgaptmp_001370"
     CDS             41966..43480
                     /gene="caiT"
                     /locus_tag="ACHKYO_RS06850"
                     /old_locus_tag="pgaptmp_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HGU4428241.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            43511..44653
                     /gene="caiA"
                     /locus_tag="ACHKYO_RS06855"
                     /old_locus_tag="pgaptmp_001371"
     CDS             43511..44653
                     /gene="caiA"
                     /locus_tag="ACHKYO_RS06855"
                     /old_locus_tag="pgaptmp_001371"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HGU4428242.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            44782..45999
                     /gene="caiB"
                     /locus_tag="ACHKYO_RS06860"
                     /old_locus_tag="pgaptmp_001372"
     CDS             44782..45999
                     /gene="caiB"
                     /locus_tag="ACHKYO_RS06860"
                     /old_locus_tag="pgaptmp_001372"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HGU4428243.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            46073..47626
                     /gene="caiC"
                     /locus_tag="ACHKYO_RS06865"
                     /old_locus_tag="pgaptmp_001373"
     CDS             46073..47626
                     /gene="caiC"
                     /locus_tag="ACHKYO_RS06865"
                     /old_locus_tag="pgaptmp_001373"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HGU4428244.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            47735..48520
                     /gene="caiD"
                     /locus_tag="ACHKYO_RS06870"
                     /old_locus_tag="pgaptmp_001374"
     CDS             47735..48520
                     /gene="caiD"
                     /locus_tag="ACHKYO_RS06870"
                     /old_locus_tag="pgaptmp_001374"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HGU4428245.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            48526..49116
                     /gene="caiE"
                     /locus_tag="ACHKYO_RS06875"
                     /old_locus_tag="pgaptmp_001375"
     CDS             48526..49116
                     /gene="caiE"
                     /locus_tag="ACHKYO_RS06875"
                     /old_locus_tag="pgaptmp_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HGU4428246.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(49202..49597)
                     /gene="caiF"
                     /locus_tag="ACHKYO_RS06880"
                     /old_locus_tag="pgaptmp_001376"
     CDS             complement(49202..49597)
                     /gene="caiF"
                     /locus_tag="ACHKYO_RS06880"
                     /old_locus_tag="pgaptmp_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HGU4428247.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(49858..53079)
                     /gene="carB"
                     /locus_tag="ACHKYO_RS06885"
                     /old_locus_tag="pgaptmp_001377"
     CDS             complement(49858..53079)
                     /gene="carB"
                     /locus_tag="ACHKYO_RS06885"
                     /old_locus_tag="pgaptmp_001377"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGU4428248.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGFETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(53097..54245)
                     /gene="carA"
                     /locus_tag="ACHKYO_RS06890"
                     /old_locus_tag="pgaptmp_001378"
     CDS             complement(53097..54245)
                     /gene="carA"
                     /locus_tag="ACHKYO_RS06890"
                     /old_locus_tag="pgaptmp_001378"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGU4428249.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT        13198 a        14747 c        14254 g        12397 t
ORIGIN      
        1 aattgatcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca tataattcaa
       61 tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt cctcgttgat
      121 tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt agttaagcag
      181 aaattaatat cgcttacttt aaccaccgca gcacaattag ctaattttac ggatgcagaa
      241 ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg tatccagtaa
      301 ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg ttcgctttat
      361 cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg gcatccagca
      421 ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt tttatgcccg
      481 aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt cattattttc
      541 gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt gaaagaaaaa
      601 ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt cggtttcccc
      661 gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt taaaaacagc
      721 cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc cgaatccctg
      781 aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat gcacatcgcc
      841 accaagctgc gcagcaccct ggacgaggtg atcgaacgcg ctatctatat ggtgaaacgc
      901 gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg tacacccatt
      961 gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac catcaacatt
     1021 ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag cggcctgtat
     1081 gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga cgatttaggc
     1141 ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt ggaaggtgca
     1201 atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat catggcgatt
     1261 aaagttcgta aggatattct caacgtccac accgccatta atcaccagga gatatggcgc
     1321 accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa agccattgtt
     1381 ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct gaaaaaccgc
     1441 gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca gctgaatctg
     1501 acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg gtataaagaa
     1561 agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga caaaaaaggt
     1621 caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca agaagagccg
     1681 gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat cgccaccgcc
     1741 gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg taacggtccg
     1801 gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga actggtgaaa
     1861 tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga tattgtcgcc
     1921 aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt cgagtcctcc
     1981 gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt cgaaaaagag
     2041 ttgcaacgca aagctcaaca caacgaaaac aacaaggaaa ccgtgtgatg tcgaagaatt
     2101 accatattgc cgtattgccg ggggacggta ttggtccgga agtgatgacc caggcgctga
     2161 aagtgctgga tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc cattacgatg
     2221 taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg gttgaaggtt
     2281 gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg tccgaaatgg gaacatttac
     2341 caccagacca gcaaccagaa cgcggcgcgc tgttgccttt gcgtaagcac ttcaaattat
     2401 tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt ccgctgcgtg
     2461 cagacattgc cgctaacggc ttcgacatcc tgtgcgtgcg cgaactgacc ggcggcatct
     2521 atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg tttgataccg
     2581 aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa tctgctcgta
     2641 aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc tctattttat
     2701 ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg gcgcatatgt
     2761 acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac gttctgctgt
     2821 gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact ggctcgatgg
     2881 ggatgttgcc ttccgccagc ctgaacgagc aaggttttgg tctgtatgaa ccggcaggcg
     2941 gctcagcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcgcaa attctgtcgc
     3001 tggcactgct gctgcgctac agcctggatg ccgatgatgc ggcttgcgcc attgaacgcg
     3061 ccattaaccg cgcattagaa gaaggcattc gcaccgggga tttagcccgt ggcgctgccg
     3121 ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa ggggtgtaat
     3181 catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg aagccgaaaa
     3241 cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct caccgcaggc
     3301 gtttgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa ccttcgccac
     3361 tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg aaatggcgcg
     3421 catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc tgtatgacct
     3481 gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg tcaccttgcc
     3541 ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt ttggcgcact
     3601 ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa ccctgaaaca
     3661 gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg gcattacagc
     3721 aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca ccgggcatgt
     3781 ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta tgaccctgtg
     3841 caatatggca atcgaaatgg gcgcaaaagc cggtctggtt gcaccggacg aaaccacctt
     3901 taactatgtc aaaggccgtt tgcatgcgcc gaaaggcaaa gatttcgacg acgccgttgc
     3961 ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg tcactctgca
     4021 agcagaagaa atttcaccgc aggtcacctg gggcaccaat ccaggccagg tgatttccgt
     4081 gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgcg cgtcggcaga
     4141 aaaagcgctg gcctatatgg ggctgaaacc gggcattccg ctgaccgaag tggctatcga
     4201 caaagtgttt atcggttcct gtaccaactc gcgcattgaa gatttacgcg cggcagcgga
     4261 gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc ccggctctgg
     4321 cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atctttattg aagccggttt
     4381 tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc gtctgaatcc
     4441 gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg ggcgcggcgg
     4501 gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg gacatttcgc
     4561 cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc aaacacacag
     4621 gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc ccgaaacagt
     4681 ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac tggcgttttc
     4741 tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg cagtatcagg
     4801 gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt gagcacgcgc
     4861 cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt gctgacatct
     4921 tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat gcagaagtgg
     4981 acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg gatctggaag
     5041 cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc ttccgccgcc
     5101 actgcatgat gaacggtctg gacagtattg ggcttacctt gcagcacgac gacgccattg
     5161 ccgcttatga agcaaaacaa cctgcgttta tgaattaatc cccttgcccg gtcaaatgac
     5221 cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt taaaaataat
     5281 gcgacaacag aaataaccgc aattacccag tagacagcaa agtgccccca actttgtgca
     5341 attgctccct gaataacgcc agccagaatt accccggtag aaatactgtt agtaaataag
     5401 gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat cccaataccc
     5461 gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc catacggcta
     5521 tgaaagaaaa tcaatccggt gtaaaacagt actcctgccg ccactgctat gaccatcatt
     5581 cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg tatttccagt
     5641 ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa ctcgctactg
     5701 atccacaacg gcatatcaat aatgtacatg gtgttgcagg tccacattaa cgtcgaggcg
     5761 acaaataaca tccgtacgtt actatcctgc cagccacctt gcattgataa agcattttcc
     5821 gacggcagtt ctacccgcgc cacagacgga agcataaatg caatcaatac cagactgagt
     5881 gtgaatatcc cggcggcaat cgaaaacatc accgtaaagc cgtaattcaa cgccagcata
     5941 aaggccaacg gtggaccgat aacccatgcc agagaaagct gcgcacgcat caccgagcta
     6001 aacatcacca cttctcgcgc cgagttatcc gcatattccc gcgccagagc aaataactgt
     6061 ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag cgtcagataa
     6121 tgacgattaa atgcaaacaa tagcgcattg ccgatagcca tcaaacagca aaatataatc
     6181 agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct tacgccgatc
     6241 ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc gccaacctca
     6301 cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc catcataaaa
     6361 gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt cattatccag
     6421 atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta taatctgctg
     6481 gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa ctttcagaat
     6541 tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg acgcttaacc
     6601 aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac tgacgcatgg
     6661 ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa taaaattgct
     6721 gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa cagttcatcc
     6781 gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa ctggcagcgt
     6841 tattgagctg ctcgcgtcgt catatgcgca ccctgctcaa caccatgcag gatcgcggct
     6901 ggctgacgtg ggaagcggaa gtcgggcgtg gtaaacgctc gcgtctgaca ttcctctata
     6961 ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat cgtatcgacc
     7021 aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt tctcatctgg
     7081 gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt ccgttgcgta
     7141 atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg caaatcttca
     7201 gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc gcccaccact
     7261 ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc cattttcatc
     7321 atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc aatacgctgc
     7381 cgctctattc gcatattgcg gacattgtgt cgccgacgcc ctggacgctg gatatccatc
     7441 tcacgcaacc ggaccgctgg ttaccgttac tgctgggaca agttccggcg atgatcctgc
     7501 cgcgcgaatg ggaaaccctc agtaactttg ccagccatcc catcggcacc ggtccgtatg
     7561 cggtgatccg caacaccacc aatcaactga aaattcaggc attcgatgac ttcttcggtt
     7621 accgggcatt aatcgatgaa gttaacgtct gggttctgcc ggaaattgcc gacgagccag
     7681 ctggaggtct gatgctaaaa ggtccacagg gtgaggaaaa agagattgaa agccgcctgg
     7741 aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg aatcagcaag
     7801 tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc gctgaggaac
     7861 agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac catgcccgca
     7921 ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt tatcaggatc
     7981 acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt caccaggtca
     8041 cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc gagagcgata
     8101 tctggcttaa cagcgccaac tttacgctgc cgctggattt ttcgctgttc gcgcacttgt
     8161 gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac gccgcccgct
     8221 ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc agcaaagcaa
     8281 tggtgccgct tatccaccac tggctgatca ttcaggggca acgcagtatg cgcggcctgc
     8341 gcatgaacac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca ccggatccat
     8401 gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg tgccacgcaa
     8461 acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc gaagggattt
     8521 gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt aaaaaaatgt
     8581 cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct gactgtttat
     8641 acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa agcttttgaa
     8701 gccgactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc gcttctcaac
     8761 cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct ggataacaat
     8821 ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc agcggatgcc
     8881 gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta cggctatttc
     8941 gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa agaattggtt
     9001 gagagcgatc agaactggcg ggtgatttat caggatccgc gcaccagtac gccggggctg
     9061 ggcctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc ctggcaaaaa
     9121 ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg cctgttttta
     9181 aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca cattctcgaa
     9241 gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct gcaggtggaa
     9301 gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt cctccagttt
     9361 atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta tccggtggca
     9421 aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac gctggagttc
     9481 acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca acgcgccgtc
     9541 agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg gtggtagcgg
     9601 ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac tgggtggcag
     9661 tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag gcgtttctct
     9721 cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat cgcaggcgct
     9781 ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc ccggtgctag
     9841 ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca tcgctctgcc
     9901 aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt ttgctggcgc
     9961 acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg gaaaacattc
    10021 ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat tttttccgct
    10081 tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt atctttatgc
    10141 tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag gcaactacta
    10201 tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc gcggcgatgc
    10261 tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag cgactaagta
    10321 aggccattgc gcccggtacc acgctgctgc aaggctggcg cgatccggac gatcgtctgc
    10381 atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg ctgccaccgt
    10441 tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg gcacaaccgg
    10501 tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt gtattgtgcg
    10561 tagtgctgac catgatgctg ctatggagca gtcgcgaact tcgggcgcgg cagaaaatgc
    10621 tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg gggattgtgc
    10681 tggctaccgg cttcttttta ctgctcaaca acaccattgg cctgccgcaa tctgctgacg
    10741 gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa gtactggaaa
    10801 acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg ggcattgaag
    10861 gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg gcgcaggcgc
    10921 tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg ttcggtaacg
    10981 atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat cgcagccagg
    11041 acggtgcggt caccgcgtta attctgctac tactctgttt tctgctgttt accgtgattg
    11101 aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta tcaccatttg
    11161 ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct cgggccaagc
    11221 ggcgcgggta aaagtaccct gctgaatttg atcgccggtt ttctgacgcc agccagcggt
    11281 tcgctgacta tcgacggcgt tgatcacacc acgacgccgc cgtcacgccg tccggtgtcg
    11341 atgctgtttc aggaaaacaa cctgttcagt cacctgacgg tcgcgcagaa catcgggctg
    11401 gggctaaatc cgggattgaa actgaacgcg gcacagcagg agaaaatgca cgctatcgcc
    11461 cgccagatgg ggattgataa tttaatggcg cggttacctg gcgagctttc cggcggtcag
    11521 cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt attgctcgat
    11581 gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct ggtgagcacg
    11641 agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga agatgcggcg
    11701 cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca gggtaagacc
    11761 aatgagttgt tgagcggtaa ggcgagtgct tcggcactat tggggattac gggttagtat
    11821 gcactgtcgg atgcggcgtg aacgccttat ccgacctacg ggtagtgcgt tttgtaggcc
    11881 tgataagacg cggtcagcgt cgcatcaggc aacccgcaca agactatctc ctaaccccca
    11941 accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat taacaccacc
    12001 agcgccacca cgccgatggc agaaattaac ggcgtcagcc acaacaaacg accacgggac
    12061 aaataatgac tcaaacggtc agtcgcttta ccactgcgcc ataaccgcca gcacagccag
    12121 ccaccaaccc acaaaaacac cgctgttgcc agcagcaacc atttaaactc accgctctgc
    12181 attccggctg gaatatcgat cgccgcgccc gccagaatcc ctggcaggaa gtaaaacggt
    12241 ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg cagatccagc
    12301 attcccgcca ccattggcac cagcggacgc gtcgggccaa caaaacgacc gaccagaatg
    12361 gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc tttgtttttc
    12421 ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca gaaggagatc
    12481 cagtcgccca gcaggcaacc gacgatccct gccagccagg cgtgccagaa acttaactcg
    12541 ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag aatcaaaccg
    12601 accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga atacacggta
    12661 gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa tggacgaagc
    12721 agggattctg caaaccctat gctactccgt caagccgtca attgtctgat tcgttaccaa
    12781 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggctc ggaactcgct
    12841 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
    12901 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
    12961 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
    13021 atgtgacaga cgcgacggtg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
    13081 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
    13141 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
    13201 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
    13261 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa
    13321 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
    13381 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
    13441 cagcaaaata tcacccggtc ggcatacaaa ttctcgtccc tgatttttca ccaccccctg
    13501 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
    13561 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
    13621 gtatcccggc agcaggggat cattttgtgc ttcagccata cttttcatac tcccgccatt
    13681 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
    13741 gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
    13801 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
    13861 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
    13921 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
    13981 ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt gattctgtgc
    14041 gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta gagtggtatc
    14101 cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt catcatccgc
    14161 gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag cttagcgtcg
    14221 aacagcgcgc agctgtggtc gggattggcg ttgacacaac cggctcgacg cccgcaccga
    14281 ttgatgccaa cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac ccgaacgcga
    14341 tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt acccgtttgt
    14401 gccacgcgcc gggcaacgtt gactactccc gctacattgg cggtatttat tccagcgaat
    14461 ggttctgggc aaaaatcctg catgtgactc gccaggacac cgccgtggcg caatctgccg
    14521 catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc acccgcccgc
    14581 aggatattcg tcgcggacgt tgcagcgccg ggcataagtc gctatggcac gaaagctggg
    14641 gtggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat cgccatttgc
    14701 cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc ttatgcccgg
    14761 aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc gcgtttgact
    14821 gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa gttatcggta
    14881 cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg gcagtgaaag
    14941 gtatttgcgg tcaggttgat ggcagcgtgg tacctggatt tatcggtctg gaagcaggcc
    15001 aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg ccgctggaac
    15061 agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag aaacaactgc
    15121 ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg ccggtggtgc
    15181 tcgactggtt taacggccgc cgcacaccga acgctaacca acgcctgaaa ggggtgatta
    15241 ccgatcttaa cctcgctacc gacgctccgc tgctgttcgg cggtttgatt gctgccaccg
    15301 cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc gtcaataacg
    15361 tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc tgctgcgacg
    15421 tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc ggtgcggcga
    15481 tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag caaaaaatgg
    15541 ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc tttgaacagc
    15601 tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt ccaacttccg
    15661 ccccggcaca ggcagcccag gccgttgcga ctctataagg acacgataat gacgattttt
    15721 gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc ggaaaccctg
    15781 cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga agcgaaactg
    15841 ccctgcaaac tagtgttgaa accgctgggc accacgccgg atgaaatcac cgctatttgc
    15901 cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca cactttctcc
    15961 ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct gcaattccac
    16021 acccagttta acgcggcgct gccgtgggac agtatcgata tggactttat gaacctgaac
    16081 cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg tcagcaacat
    16141 gccgtggtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg ctcctggatg
    16201 cgccaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgttt tggcgataac
    16261 atgcgtgaag tagcggtcac cgatggtgat aaagttgccg cacagatcaa gttcggtttc
    16321 tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat cagcgacggc
    16381 gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc tgccacacaa
    16441 atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct ggggatgaag
    16501 cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga tttgcacggt
    16561 ctgaaacagc ttccaggtct ggccgtacag cgtctgatgc agcagggtta cggctttgcg
    16621 ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat gtcaaccggt
    16681 ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa aggtaatgac
    16741 ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga agagaaaccg
    16801 atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg actgatcttc
    16861 aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga tcgttaccgt
    16921 ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa actgccggtg
    16981 gcgaatgcgc tgtggaaagc gcaaccggat ctgccaactg cttccgaagc gtggatcctt
    17041 gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga tatgcgccag
    17101 ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg cctgccagcg
    17161 tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta agtagccaca
    17221 tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca cgctgcgatt
    17281 ttgtaggccg gataagcaaa gcgcatccgg cattgttttc cccctcgccc ctttggggag
    17341 agggccgggg tgaggggaaa cgacctctca ggagtcaaca tgttagaaga tctcaaacgc
    17401 caggtattag aagccaacct ggcgctgcca aaacacaacc tggtcacgct cacatggggc
    17461 aacgtcagcg ccgttgatcg cgagcgcggc gtctttgtga tcaaaccttc cggcgtcgat
    17521 tacagcgtca tgaccgctga cgatatggtc gtggttagca tcgaaaccgg tgaagtggtt
    17581 gaaggtacga aaaagccctc ctccgacacg ccaactcacc ggctgctcta tcaggcattc
    17641 ccctccattg gcggcattgt gcatacgcac tcgcgccacg ccaccatctg ggcgcagtcg
    17701 ggtcagtcga ttccagcaat cggcaccacc cacgccgact atttctacgg caccattccc
    17761 tgcacccgca aaatgaccga cgcagaaatc aacggcgaat atgagtggga aaccggtaac
    17821 gtcatcgtag aaacctttga aaaacagggt atcgatgcag cgcaaatgcc cggcgttctg
    17881 gtccattccc acggcccgtt tgcatggggc aaaaatgccg aagatgcggt gcataacgcc
    17941 atcgtgctgg aagaggtcgc ttatatgggg atattctgcc gtcagttagc gccgcagtta
    18001 ccggatatgc agcaaacgct gctggataaa cactatctgc gtaagcatgg cgcgaaggca
    18061 tattacgggc agtaatgact gtataaaacc acagccaatc aaacgaaacc aggctatact
    18121 caagcctggt tttttgatgg attttcagcg tggcgcaggc aggttttatc ttaacccgac
    18181 gctggcggga caccccgcaa gggacagaag tctccttctg gctggcgacg gacaacgggc
    18241 cgttgcaggt tacgcttgca ccgcaagaat ccgtggcgtt tattcccgcc gatcaggttc
    18301 cccgcgctca gcatattttg cagggtgaac aaggctttcg cctgacaccg ctggcgttaa
    18361 aggattttca ccgccagccg gtgtatggcc tttactgtcg cgcccatcgc caattgatga
    18421 attacgaaaa gcgcctgcgt gaaggtggcg ttaccgtcta cgaggccgat gtgcgcccgc
    18481 cagaacgcta tctgatggag cggtttatca cctcaccggt gtgggtcgag ggtgatatgc
    18541 acaatggcac tatcgttaat gcccgtctga aaccgcatcc cgactatcgt ccgccgctca
    18601 agtgggtttc tatagatatt gaaaccaccc gccacggtga gctgtactgc atcggcctgg
    18661 aaggctgcgg gcagcgcatc gtttatatgc tggggccgga gaatggcgac gcctcctcgc
    18721 ttgatttcga actggaatac gtcgccagcc gcccgctgct gctggaaaaa ctcaacgcct
    18781 ggtttgcaaa ccacgatccc gatgtgatca tcggctggaa cgtggtgcag ttcgatctgc
    18841 gaatgctgca aaaacatgcc gagcgttacc gtcttccgct gcgtcttggg cgcgataata
    18901 gcgagctgga gtggcgcgag cacggcttta aaaacggcgt cttttttgcc caggctaaag
    18961 gtcggctaat tatcgacggt atcgaggcgc tgaaatccgc gttctggaat ttctcttcat
    19021 tctcgctgga aactgtcgct caggagctat taggcgaagg aaaatctatc gataacccgt
    19081 gggatcgaat ggacgaaatt gaccgccgtt tcgccgaaga taaacctgcg ctggcaactt
    19141 ataacctgaa agattgcgag ctggtgacgc agatcttcca caaaactgaa atcatgccat
    19201 ttttactcga acgagcaacg gtgaacggcc tgccggtgga tcggcacggc ggttcggtgg
    19261 cagcgtttgg tcatctctat tttccgcgaa tgcatcgcgc tggttatgtc gcgcctaatc
    19321 tcggcgaagt gccaccgcac gccagccctg gcggctacgt gatggattca cggccagggc
    19381 tttatgattc agtgctggtg ctggactata aaagcctgta cccgtcgatc atccgcacct
    19441 ttctgattga tcccgtcggg ctggttgaag gcatggcgca acctgatcca gagcacagta
    19501 ccgaaggttt tctcgatgcc tggttctcac gggagaaaca ttgcctgccg gagattgtga
    19561 ctaacatctg gcacgggcgc gatgaagcca aacgtcaggg taataagccg ctttcgcagg
    19621 cgctgaagat cattatgaat gccttttatg gcgtgctcgg caccaccgcc tgccgcttct
    19681 tcgatccgcg cctggcatcg tcgatcacca tgcgtggtca tcagatcatg cggcagacca
    19741 aagcattgat tgaagcgcag ggctacgacg ttatctacgg agataccgac tcgacttttg
    19801 tctggctaaa aggtgcacat tcggaagaag aggcggcgaa aatcggtcgt gcactggtgc
    19861 agcacgttaa cgcctggtgg gcggaaacgc tgcaaaaaca acggctgacc agcgcattag
    19921 aactggagta tgaaactcat ttctgccgtt ttctgatgcc aaccattcgc ggagccgata
    19981 ccggcagtaa aaagcgctac gccgggctga ttcaggaggg cgataaacag cggatggtgt
    20041 ttaaagggct ggaaaccgtg cgcaccgact ggacgccgct ggcccagcag tttcagcagg
    20101 agctatacct gcgcatcttc cgcaacgagc catatcagga atatgtacgc gaaaccatcg
    20161 acaaactgat ggcgggtgaa ctggatgcgc gactggttta ccgtaaacgc cttcgccgtc
    20221 cgctgagcga gtatcagcgt aatgtgccgc ctcatgtacg cgccgctcgc cttgccgatg
    20281 aagaaaacca aaagcgcggt cgccccttgc aatatcagaa tcgcggcacc attaagtacg
    20341 tatggaccac caacggcccg gagccgctgg actaccaacg ttcaccactg gattacgaac
    20401 actatctgac ccgccagcta caacccgtgg cggagggaat actccctttt attgaggata
    20461 attttgctac acttatgacc gggcaacttg ggctattttg agcaaaaaaa agagttcgcc
    20521 agataccatt ttgatgcgtg acgaatgctt tgccatccag taccatagcg ccctttccat
    20581 tcctggacct gaataacacc actacctcat aagcacggta gcgggtggtt attgcctgca
    20641 attaaagata tagagccgaa cacatatgcc ttttacactt ggtcaacgct ggatcagcga
    20701 tacagaaagc gaattgggac ttggaaccgt tgtcgcggtg gatgcgcgaa ctgtcacttt
    20761 acttttccca tctactggtg aaaaccgtct gtacgcacgc agtgattccc ccgtgacccg
    20821 cgtgatgttc aaccctggtg atactattac cagccatgac ggctggcaga tgcaagtcga
    20881 agaagtaaaa gaagaaaatg gcttgctgac ctatatcggt actcgcctgg atactgaaga
    20941 gtccggcgta gccctgcgtg aagttttcct tgatagcaaa ctggtgttca gcaaaccgca
    21001 ggaccgtctg tttgccgggc agattgaccg tatggaccgc tttgcgctgc gttatcgcgc
    21061 gcgtaagtat tccagcgaac agttccgtat gccgtacagc ggcctgcgcg gtcagcgtac
    21121 cagcctgatt ccgcatcagc tcaacatcgc tcatgatgtt ggtcgccgcc acgcgccgcg
    21181 cgtcctgctg gctgacgaag tgggtttagg gaaaaccatt gaagccggga tgatcctgca
    21241 tcagcaactg ctctctggcg ctgctgaacg tgtgctaatt atcgtcccgg aaaccttaca
    21301 gcatcagtgg ctggtagaaa tgctgcgccg tttcaacctg cgctttgcgc tatttgatga
    21361 tgagcgttat gccgaagctc agcacgatgc ttacaacccg tttgacaccg aacagctggt
    21421 gatttgctcg ctggattttg cccgtcgtag caaacagcgc ctggaacatc tctgtgaagc
    21481 cgaatgggac ctgctggtgg tcgatgaagc gcatcacctg gtgtggagcg aagatgcgcc
    21541 aagccgtgaa tatcaggcca ttgaacaact ggcagagcac gtgccgggcg ttctgctgct
    21601 gaccgcgacc ccggaacagc tggggatgga aagccacttc gcccgtctgc gtctgctgga
    21661 cccgaaccgt ttccacgatt ttgcgcagtt cgttgaagag cagaaaaatt atcgtccggt
    21721 tgcggacgcc gttgccatgc tgctggcagg taacaaactg agcaatgacg aactgaacat
    21781 gctcggcgag atgatcggcg agcaggatat cgagccgctg ttgcaggcag caaacagcga
    21841 cagcgaagat gcccagagcg cccgtcagga gctggtttcg atgctgatgg atcgccacgg
    21901 caccagccgc gtgctgttcc gtaacacgcg taacggtgtg aaaggattcc cgaaacgcga
    21961 gctgcacacc attaaactgc cgctaccgac gcagtatcag acggctatta aagtctccgg
    22021 cattatgggc gcacgtaaaa gtgcggaaga tcgtgctcgc gatatgctct acccggagcg
    22081 tatttatcag gaatttgaag gtgataacgc cacctggtgg aacttcgatc cgcgcgttga
    22141 gtggctgatg ggctacctga ccagccatcg ctctcagaaa gtgctggtga tctgcgccaa
    22201 agctgccact gcgctgcaac tggagcaggt actgcgcgaa cgtgaaggta ttcgcgctgc
    22261 ggtgttccac gaaggtatgt cgattatcga acgtgaccgc gctgccgcct ggtttgccga
    22321 agaagacacc ggcgcacagg tactgctgtg ctcagaaatc ggttctgaag gacgtaactt
    22381 ccagttcgcc agccacatgg tgatgtttga cctgccattc aacccggatc tactggagca
    22441 gcgtattggt cgtctggatc gtatcggcca ggcgcacgat attcagatcc atgtgcctta
    22501 tctggagaaa accgctcagt cggtgctggt gcgctggtat cacgaaggtc tggatgcatt
    22561 tgagcacacc tgcccgaccg gacgcactat ttacgatagc gtatacaacg atctgattaa
    22621 ctatctggct tcaccggatc aaaccgaagg ctttgacgat ctgatcaaaa actgccgcga
    22681 gcaacatgaa gcgctgaaag cacaactgga acagggtcgt gaccgcctgc tggaaatcca
    22741 ctccaacggt ggcgaaaaag cccaggcact ggcagaaagc attgaagagc aggatgacga
    22801 taccaacctg atcgccttcg ccatgaacct gttcgatatt atcggtatca atcaggacga
    22861 tcgcggcgac aacatgatcg tgctgacgcc gtccgatcat atgctggtgc cggacttccc
    22921 tggcctgtcg gaagatggca tcaccatcac ctttgatcgt gaagtggcgc tggcgcgtga
    22981 agatgctcag tttattacct gggaacatcc gctgatccgc aacggtctgg atctgatcct
    23041 ttctggcgat accggtagca gcacgatttc actgttaaaa aacaaagcgt tgccggtagg
    23101 tacgctgttg gtggaactga tttacgtggt cgaagcccag gcgccgaagc agttgcagct
    23161 caaccgcttc ctgccaccga cgccggtacg tatgctgctg gataaaaacg gcaacaacct
    23221 ggcggcccag gtggagtttg aaacctttaa ccgccagctt aacgcggtta accgtcacac
    23281 cggcagcaaa ctggttaacg ccgtgcagca ggatgttcac gctatccttc agctgggtga
    23341 agcgcagatc gagaaatctg cccgtgcatt gattgatgcc gcacgtaacg aagccgacga
    23401 aaaactgtct gccgagctgt ctcgtctgga agcgctgcgc gcagtgaacc cgaacattcg
    23461 tgacgacgaa ctgaccgcca ttgagagcaa ccgtcagcag gtaatggaaa gcctggatca
    23521 ggcaggctgg cgtctggatg ccctgcgttt gatcgttgta acgcatcagt aacggagccg
    23581 aaaatgggga tggaaaacta caatccaccg caggaaccct ggttggttat cctgtatcag
    23641 gatgaccaca ttatggtggt taacaagccg agcggtttgt tgtctgtgcc gggtcgtctg
    23701 gaagagcaca aagatagcgt gatgacgcgc attcagcgtg attatccgca ggcagaatcg
    23761 gtgcatcgtc tggatatggc taccagcggc gtgattgtag tggcgctgac caaagccgcg
    23821 gagcgggagt taaaacgcca gttccgcgag cgcgagccga aaaagcagta tgtggcccgc
    23881 gtctgggggc atccatcccc cgcagaaggt ctggtggatc tgccgctgat ttgcgactgg
    23941 ccaaaccgcc cgaaacagaa agtctgttac gaaacgggta aacctgcgca gacggaatat
    24001 gaagtggtgg agtatgcggc ggataacacg gcaagagtgg tgttaaaacc gattaccggg
    24061 cgttcgcatc aattgcgtgt gcatatgctg gcgctggggc atccgattct cggcgatcgt
    24121 ttttatgcat caccagaagc gagagcgatg gcaccgcgtt tgttgctaca tgcagagatg
    24181 ttgacgatta cccatccggc gtatggcaat agtatgacgt ttaaagcgcc agcggatttt
    24241 taatcgtcac gctggcgcag ttcggtaggc ggataaggcg ttcacgccgc atccggcaat
    24301 tgtgcgccga ttgcctgatg cgacgcgggc cgcgtcttat caggcctaca gtttaaaact
    24361 tatttaaacc ctttctgctg ctttatcagc tcatatgcct gctgaatttc ctgcgctttc
    24421 tgcttcgcca tctccatcat ctcaggcggc aaacctttcg ccaccagctt atcgggatgg
    24481 tgttcactca tcagcttacg gtaggcacgt ttgatggtgg tcgcatcatc cgtcggcttc
    24541 acgcccagca cattacaggc atcttccagc gtcgggccac gctgcgcttg ctgccagtta
    24601 ccaccgccag attgctgctg ataaccgccg ccaaactgtg caccgccctg catcatgcgc
    24661 aaaaactggt caaactgggc gcgggagatc cctaattctt cagcaatgac atacagcacc
    24721 gcccgttcat tcgggtgcag tgaaccatca gcaaacgccg cctgaatctg gatctccaga
    24781 aacatacgaa ttaagtcaaa acgaccaaag cagacactgc gaaactggcg catcttttcg
    24841 cgcagcgggt aattgtctga ttttcccacc cggaacgcat tttgcgccgc agtacgggaa
    24901 gcgccatgaa gattcattcg gtccatcaac tggctggcga tatgaatatc agcctccgtg
    24961 acgcgacctt tggatttggt taaatgcccc atcacttcaa aagtggtggc aaaaaacagc
    25021 gcctgacgct cacgctggtt ggcgaaccac gccattttac ggctacgggc tttatcaaac
    25081 atatggccaa ttaacaggcc taacactacg ccccaaaagc cgccgcccat cagtaaggcc
    25141 acggccacgc caatgatttt tccccaatac tgcatatatt ccccaaatcg acacacggat
    25201 atcagggcta tctcccacaa tataaaggtg cttttaccgt tttccggctg cggtcaattg
    25261 tgggacatcg cctataattt gcattatcat acctgtcatt caatgccgtg cctaacacca
    25321 ctgacgctat tcggacagga ttaacactag cgtagagatg acgagtacgt tagtctctga
    25381 ccgtttgtca cgcgcaacgt taccgatgat ggaacaataa aatcaacgta tgaaaaaacg
    25441 tatccccact ctcctggcca ccatgattgc caccgccctt tatagtcaac agggactggc
    25501 agccgacctc gcctcacagt gcatgttggg cgtgccaagc tatgaccgtc ctctggtaca
    25561 gggcgatacc aatgacttac ccgtgactat caatgctgac cacgcgaaag gggactaccc
    25621 ggatgacgcc gtgtttactg gcagcgtgga tatcatgcag ggtaacagcc gtctgcaggc
    25681 cgacgaagtg cagctccatc aaaaagaggc accaggacaa ccggagccgg tacgtaccgt
    25741 tgatgcgctc ggtaatgtcc attacgacga taaccaggtg atcctcaaag ggccgaaagg
    25801 ctgggcgaat ctgaacacca aagataccaa cgtctgggaa ggtgattacc agatggtggg
    25861 tcgccagggt cgcggtaaag cggacctgat gaaacaacgt ggcgaaaacc gctataccat
    25921 tctggataac ggtagcttta cctcctgtct gccgggttct gacacctgga gcgtggtagg
    25981 tagcgaaatt attcatgacc gcgaagaaca agttgcggag atctggaacg cccgctttaa
    26041 ggtgggtccg gtaccgatct tttatagccc ctatttgcag ttgccggtgg gtgacaaacg
    26101 tcgctctggt ttcttgatcc cgaacgccaa gtacaccacc accaactact ttgagttcta
    26161 cctgccatat tactggaaca tcgcgccaaa tatggatgcc accatcacgc cgcattatat
    26221 gcatcgtcgt ggcaacatca tgtgggagaa cgaattccgc tacctctccc aggcgggcgc
    26281 tggcttgatg gaactggact atctgccttc agataaagtc tatgaagatg aacacccgaa
    26341 cgatgacagt tcacgtcgtt ggttattcta ctggaaccac tccggggtca tggatcaagt
    26401 gtggcgtttc aacgtcgact acaccaaggt cagcgatcct agctacttca atgatttcga
    26461 taacaagtac ggttccagta ctgacggcta cgcaacgcaa aaattcagcg ttggctatgc
    26521 ggtgcaaaac ttcaatgcca ccgtttcaac caagcagttc caggttttca gcgaacagaa
    26581 caccagtagc tactcggcag agccgcagtt agacgttaat tactaccaga atgatgttgg
    26641 tccgtttgat acgcgtattt acggccaggc agtgcacttt gttaacacca gagacgacat
    26701 gcctgaagca acccgtgttc acctggaacc gaccatcaat ttgccgctct ctaataactg
    26761 gggcagcatc aataccgaag cgaagttgct ggcaacccat tatcagcaaa ccaatcttga
    26821 ctggtataac tccagaaaca cgaccaagct ggacgaatcc gttaaccgcg taatgccgca
    26881 attcaaagtt gacggcaaaa tggtctttga acgcgatatg gaaatgctgg ctccgggtta
    26941 tacccaaacg ctggaaccgc gcgcgcagta tttgtacgtg ccgtatcgcg atcagagcga
    27001 catctataac tacgactcgt ctctgctgca atctgactac tctggcctgt tccgggaccg
    27061 gacttacggc ggtcttgacc gtattgcctc cgctaaccag gtgacgaccg gtgtcacatc
    27121 tcgcatatat gatgatgctg ccgttgaacg ttttaatatt tccgttggtc aaatctacta
    27181 tttcacggag tctcgcactg gcgatgacaa cataacatgg gagaatgacg acaaaacggg
    27241 ttcactggtg tgggcaggcg atacttactg gcgtatctcc gagcgttggg gattgcgtgg
    27301 cgggattcag tacgatacac gtctggataa cgtagcgacc agtaactcca gcattgaata
    27361 ccgtcgggat gaagaccgtc tggtacagct gaattaccgt tacgccagcc cggaatatat
    27421 tcaggctacg ctgcctaagt actattccac tgctgagcaa tataagaatg gtatttcgca
    27481 ggtaggtgct gtcgccagct ggccaattgc cgatcgttgg tccattgttg gggcctacta
    27541 ctacgacacc aatgcgaaca agcaagccga ctctatgtta ggtgtgcaat acagctcctg
    27601 ctgctatgca attcgcgtcg gttacgagcg gaagctgaac ggttgggata acgataaaca
    27661 acatgcggta tatgacaacg caatcggctt taacatcgaa cttcgcggcc tgagctccaa
    27721 ctacggtctg ggtacgcaag agatgctgcg ttcgaacatt ctgccgtatc aaaacacttt
    27781 gtgatttgtt gatttaccac gtaatccgca gtgcggttaa ttgaaatgga aaaagtatga
    27841 agaactggaa aacgctgctt ctcggtatcg ccatgatcgc gaataccagt ttcgctgccc
    27901 cccaggtagt cgataaagtc gcagccgtcg tcaataacgg cgtcgtgctg gaaagcgacg
    27961 ttgatggatt aatgcagtcg gtaaaactga acgctgctca ggcaaggcag caacttcctg
    28021 atgacgcgac gctgcgccac caaatcatgg aacgtttgat catggatcaa atcatcctgc
    28081 agatggggca gaaaatggga gtgaaaatct ccgatgagca gctggatcag gcgattgcta
    28141 acatcgcgaa acagaacaac atgacgctgg atcagatgcg cagccgtctg gcttacgatg
    28201 gtctgaacta caacacttat cgtaaccaga tccgcaaaga gatgattatc tctgaagtgc
    28261 gtaacaacga ggtgcgtcgt cgcatcacca tcctgccgca ggaagtcgaa tccctggcgc
    28321 agcaggtggg taaccaaaac gacgccagca ctgagctgaa cctgagccac atcctgatcc
    28381 cgctgccgga aaacccgacc tctgatcagg tgaacgaagc ggaaagccag gcgcgtgcca
    28441 ttgtcgatca ggcgcgtaac ggcgctgatt tcggtaagtt ggcgattgct cattctgccg
    28501 accagcaggc gctgaacggc ggccagatgg gctggggccg tattcaggag ttgccgggga
    28561 tcttcgccca ggcattaagc accgcgaaga aaggcgacat tgttggcccg attcgttccg
    28621 gcgttggctt ccatattctg aaagttaacg acctgcgcgg cgaaagcaaa aatatctcgg
    28681 tgaccgaagt tcatgctcgc catattctgc tgaaaccgtc gccgatcatg actgacgaac
    28741 aggcacgcgt gaaactggaa cagattgctg ctgatatcaa gagtggtaaa acgacttttg
    28801 ctgccgcagc gaaagagttc tctcaggatc caggctccgc taaccagggc ggtgatctcg
    28861 gctgggctac accagatatt ttcgatccgg ccttccgtga cgccctgact cgcctgaaca
    28921 aaggtcaaat gagtgcaccg gttcactctt cattcggctg gcatttaatc gaactgctgg
    28981 atacccgtaa tgtcgataaa accgacgctg cgcagaaaga tcgtgcatac cgcatgctga
    29041 tgaaccgtaa gttctcggaa gaagcagcaa gctggatgca ggaacaacgt gccagcgcct
    29101 acgttaaaat cctgagcaac taatggttaa aacccaacgt gttgtgatca ctcccggcga
    29161 gcccgccggg attggcccgg acttagttgt ccagcttgca cagcgtgagt ggccggtcga
    29221 actggttgtt tgtgccgata ccactctcct taccgaccgg gcagcgatgc tcggtttgcc
    29281 gctcaccctc cgcccttatt cccccaactc ccctgcacaa ccgcaaacta cgggcacatt
    29341 aacgctactt cctgtcgcgc tacgtgaatc tgtcactgcg gggcagttag gggttgaaaa
    29401 tgggcattac gtggtggaga cgctggcgcg agcgtgtgat ggctgtctga acggtgaatt
    29461 tgctgcgctg atcacaggcc ccgtgcataa aggcgtcatt aacgacgcag gtattccgtt
    29521 tactggtcat accgagtttt tcgaagagcg ttcgcaggcg aaaaaagtgg tgatgatgct
    29581 ggcgaccgaa gaacttcgcg tggcgctggc aacgacgcat ttaccgctgc gcgatatcgc
    29641 agatgctatc acccctgcac ttttgcacga agtgattgct attttgcatc acgatttgcg
    29701 gacaaaattt ggtattgccg aaccgcgcat tctggtctgc ggactgaatc cgcacgcggg
    29761 cgaaggcggt catatgggta cggaagagat agacaccatt attccggtgc tcgacgagct
    29821 gcgggcgcag gggatgaaac tcaacgggcc gctgcctgcc gataccctgt ttcagccgaa
    29881 atatctcgat aacgccgacg ccgtgctggc gatgtaccac gatcagggtc ttcccgtgct
    29941 aaaataccag ggcttcgggc gcggtgtgaa cattacgctg ggcctgccct ttattcgcac
    30001 atcagtggac cacggcaccg cgcttgaact ggcgggacgt ggtaaagccg atgtcggcag
    30061 ttttattacg gcgcttaatc tcgccatcaa aatgattgtt aacacccaat gaataatcga
    30121 gtccaccagg gccacttagc ccgtaaacgc ttcgggcaaa actttctcaa cgatcagttc
    30181 gtgatcgaca gtattgtttc tgccattaac ccgcaaaagg gccaggcgat ggtcgaaatc
    30241 ggccccggtc tggcggcatt gaccgaaccg gtcggcgaac gtctggacca gctgacggtc
    30301 atcgaacttg accgcgatct ggcggcacgt ctgcaaacgc atccattctt aggcccgaaa
    30361 ctgacgattt atcagcagga tgcgatgacc tttaactttg gcgaactggc cgagaaaatg
    30421 ggtcagccgc tgcgtgtttt cggcaacctg ccttataaca tctccacgcc gttgatgttc
    30481 catctgttta gctatactga tgccattgcc gacatgcact ttatgttgca aaaagaggtg
    30541 gtgaatcgtc tggttgcagg accgaacagc aaagcgtatg gtcgattaag cgtcatggcg
    30601 caatactatt gcaacgtgat cccggtactg gaagttccgc cgtcagcctt tacaccacca
    30661 cccaaagtgg attccgccgt cgtgcgcctg gttcctcatg caacgatgcc tcacccggtt
    30721 aaagatgttc gtgtgttgag ccgcatcacc accgaagcct ttaaccagcg tcgtaaaacc
    30781 attcgtaaca gcctcggcaa cctgtttagc gtcgaggtgt taacgggaat ggggatcgac
    30841 ccggcgatgc gagcggaaaa tatctctgtc gcgcaatatt gccagatggc gaactatctg
    30901 gcggagaacg cgcctttgca ggagagttaa cgatgatcaa ttcgccccga gtgtgtattc
    30961 aggttcaaag cgtctacatt gaggcacaat cttcacctga taatgaacgt tacgtttttg
    31021 cttataccgt aaccatacgc aatctggggc gagcgccagt gcagttgttg gggcgttact
    31081 ggctgatcac caatggcaat ggccgtgaaa ccgaagtcca gggcgaagga gtggttggcg
    31141 tccagccact tatcgcgcct ggcgaagagt accagtacac cagcggtgca atcattgaaa
    31201 ccccgctggg caccatgcag ggtcactacg aaatgatcga tgaaaatggc gtccctttca
    31261 gcatcgacat tcccgtattc cgactcgccg ttcccacact cattcattaa aagaatatgg
    31321 cgacatacct tattggcgac gttcatggtt gttacgatga actgatcgca ttgctgcata
    31381 aagtagaatt tacccctggg aaagataccc tctggctgac gggcgatctg gtcgcgcgcg
    31441 ggccgggttc gctggatgtt ctgcgctatg tgaaatcctt aggcgacagc gtacgtctgg
    31501 tgctgggcaa tcacgatctg catctgctgg cggtatttgc cgggatcagc cgcaataaac
    31561 cgaaagatcg cctgacaccg ctgctggaag cgccggatgc cgacgagctg cttaactggc
    31621 tgcgtcgcca gcctttgctg caaatcgacg aagagaaaaa gttggtgatg gcccacgcag
    31681 ggatcacgcc gcagtgggat ctgcagaccg ccaaagagtg cgcgcgcgat gtagaagcgg
    31741 tgctgtcgag cgactcctat cccttcttcc ttgatgccat gtacggcgat atgccaaata
    31801 actggtcacc ggaattgcgg gggctgggaa gactgcgttt tatcaccaac gcctttaccc
    31861 gtatgcgttt ttgctttcca aacggtcaac tggatatgta cagcaaagaa tcgccggaag
    31921 aagcccctgc cccactgaaa ccgtggtttg cgattcctgg ccctgtcgct gaagaataca
    31981 gcatcgcctt tggtcactgg gcatcgctgg agggcaaagg tacgccggaa ggtatatacg
    32041 cgctggatac cggctgctgc tggggtggta cattaacctg cctgcgctgg gaagataaac
    32101 agtattttgt ccagccgtcg aaccggcata aggatttggg cgagggagag gcggtcgcgt
    32161 cttaacgaat taccattcag ggaggaataa tggctgacta ttttgaaata cgtgctgatt
    32221 acaatcagca caccatcacg gtttatcagg cttacaatga tgcaatcgct gacgtcgccg
    32281 tacgtgacgg gcgatttggc gcatcttttt cattcaaccg catgacctgg attaaaccct
    32341 cttttatgtg gatgatggag cgtagcaact ggggactgaa aaaagatcaa caacacattc
    32401 tcgcaatccg aatcaaacgc accttctttg atacattgct ggaacaagcg gtgctaacca
    32461 cgccggaagc ccatgtgtat ccacatgcag gcatttggga aacgttattt gcacaagcaa
    32521 atgtgtatgt tcagtgggat cccgaacgca gtataaatgg taaaaaactg gagcaccgat
    32581 cattacagct tggtatttcc cgtaacttga tcagtcaatt taacgaagac gccattgttg
    32641 ccattgatga tctgactccg ctggtgcgaa agtgtcataa ccttttgata aacggtaaaa
    32701 caacacaggc gaaatccttc ctgccgccag aaaaaattta ccctgttagt gcagctgcga
    32761 gaaaggcttt gggaatgaaa aattcctgag acaaatacgc gcctattaag cggaaaatat
    32821 ctaagtgcga caaatcaaca gcgggcttaa taccagagaa attattctgg caatgattga
    32881 cgaagcagat gaacccccat ttgctgagca gcggacctgg catcgcaggc gaatcgcttt
    32941 tctatgtgat tgcatcagcg ttgctatcga ctacggcttc ttctgttctg tctctcagca
    33001 gtcaataatg aacataacca ggatggctga aggtgtatgg ataacagaaa gtaaaacgac
    33061 ccaactcatt gatatttggc aacaacttaa gcagctttat ccgccagaaa ctattcccta
    33121 cgtccataag ttacccacta aggaattact tgaactgcta ctttctagtc ctgatgatct
    33181 tcgaataatt gaaattcctg acatcatcgg tgatgttttt gatgaccaaa cctggattcg
    33241 gttattcaaa aaacactttg ctgatattat ccaactacga tgtgatgtgt aacaatccaa
    33301 aatatatcgc cagacgatag accaaaaaga aagtatttag cagatactca acattctgac
    33361 ttctgtacag aaatagcaga gtttaaaaac ttaaaacttc cggtactcat cagagaaaca
    33421 tcctgtttct tctgcaaatt catttaatgc cgagatggca tcctcgttta cttcattcca
    33481 ctgtagagtg gcttgaaggt ttagacctac gcaatgagcg cgactcacaa aatcgcgctc
    33541 actggtaaaa tcaaacgtct tatcgttcaa tttgtagtcc tggcaaaaaa ttaccgccgc
    33601 tccagaatct caaagcaata gctatgagag ttctgcgcat cggcgtcgtg gaattcgctg
    33661 aataccgatt cccagtcatc cggctcgtaa tccgggaaat gggtgtcgcc ttccacttct
    33721 gcgtcgatat gcgtcagata cagtttttgc gcttttggca ggaactgctc ataaacgcga
    33781 ccaccgccaa tcaccatgat ttctggtacg tcaccacacg ccgcgatggc ttcatccacc
    33841 gacttcaccc acgttacgcg atcgtccgta cccggttgac tgctgaggat aatatttttg
    33901 cgtcctggca gcggacgacc gatggattcc caggtatggc ggcccataat tacgggttta
    33961 tttaaggtgt tgcgtttaaa ccaggcgaga tcggcaggca ggttccacgg catggcgttt
    34021 tccatgccga taacacgatc taccgctaac gccgcaatca gactaatcat tgatttatcc
    34081 tggatataaa aaattgtcgc cactatacgt aaagcataaa ccgtcgtcga ctggcgcaag
    34141 gatgatgttg agtaaaattt tacgttctgc tggcgagtcc acgctctccc actggactcg
    34201 ccacattaca atgaaacaaa aacaaacagt tagctgtaaa gtgtgattta cgtcactctt
    34261 tattaggatg agggtttcgt ttccggttca tccgccatgt tgccggtatg tttcccttct
    34321 tcggttccct gccagccatg tcgttgaatt aatgacagat gttcgcggtc ttcggtaatg
    34381 atctcactta acatcgcgct ggtgcgttta tagaccgctg cgcgggcttt ggtgtcgttc
    34441 tcaaccattg ccatctcttc caccatctga atattaaagc ggcggaacac atcggcacgt
    34501 tctcgcgctt catacggccc cagacctaaa ctttccagtg ccagacgccc ggttttcagc
    34561 gcaccttcga aggtttcacg ctccggcttt tcaacgcctg cctgacgcaa acgaatgtag
    34621 tgatcgacat cgcgggcgcg ggcaataatc tgcaaatgcg ggaaatgttc tttcaccatc
    34681 tccgtcagtt gcaggttggt ttgcggatcg tcaatggcgt taatcagcac ttccgctttc
    34741 gccgctccgg cagactccag taaatccatc cgcgtggcat cgccataaaa cactttcata
    34801 ccaaatttac gcaaggtttc gatatggtcc gggtcgtgat cgagcaccac cattttcacc
    34861 ccgctggaga gcagtaaacg tccggtgatc tgcccgaaac gaccgaatcc ggcgataatc
    34921 acgcgtggct gttcttcgtc gatctcatcg gcttcacgcg cttcctcagt agaagattgc
    34981 tcaaggcgat tgaggatcac cagcagaatc ggcgttactg ccatcgacag cgccaccgcc
    35041 agggtcagcg atttcgccca ctccggctcc agcacattcg ccatctgcgc cgcaccaaat
    35101 accacaaagg caaactcact accctgccct aacaacaccg caaaccaacg acgctgttta
    35161 tttggcactt gcaacggtcg ggcaatcagc cacagcatgg cgattttgat gatgaggaaa
    35221 ccgagcagca aaatgacaat gcgcaatggg ttttcaagca gcgtgccaaa gtctatcgac
    35281 atgccaacac cgatgaaaaa cagccccaac agcaaacctt taaatggttc gatatcgctc
    35341 tccagcgcat gacggtattc cgagcttgcc agcagtacgc ccgccagaaa cgcgcccatc
    35401 gccatcgaca agccgacctc ttccagcagc aaaccaaagc caaacacgag gaataacgcc
    35461 acggcactaa acacttcccg caagccagag cgggctacaa aacgcagcgc cggacgcgtg
    35521 acatagcgcc ccagcaatac caccagcacc agcgcacccg ccacttttaa cgccgagaga
    35581 gcaaatgcgc ccatcgtcgt cgaggcactg ctcgttgcca gtagcggaat catcgccacc
    35641 agcgggatcg ccgcgatatc ctggaacagc agcaccgcaa aggcactgcg acccatttgc
    35701 gtcaccatca gattgcgttc attcatcgcc tgcatggcaa tcgccgtaga ggagagcgcc
    35761 agcgtcatgc cgatcaattc cgcgacctgc cagcgcagcc caagtaacat gcagaacagc
    35821 cccagcaggc caccgcaaat caccatctgc aatgcgccgc cgccgaacac tgccgcacgc
    35881 agcttccata gcctttgtgg atcgagttcg aggccgataa taaacagcat cagcaccacg
    35941 ccaatctcgg caaagtgcag gatagattcg gcatcggtca ccagtcgcag cccccacggg
    36001 ccaataatgc agccggcaat caggtagcca agtaccgatc ccagaccaag acgtaccgca
    36061 atgggtacaa tcagcgccgc cgatccgagg tagatcagcg cctgaaccag cgtatggcta
    36121 tccatgatgg gcctcctgcc attccagcag acgttgctta tagtgacgcg cctgcccttc
    36181 gagggtttcg tcgtcacaaa taaaggtgca gtgcatggca aacggtggca gccagttcag
    36241 cccgcagtag attgccgtcg cctgtagcgg ctgcgacagc acatcaaagc ccggatgcgc
    36301 accaatttca aaatggcttt ccccgccgcc ggtcgtcacc gcccacagca aatgtttgcc
    36361 atgcagcgcc gtgccgccgt gaccgtaagc ccagccgtgc gagaaaactt tatcgatcca
    36421 aagtttgagg agcggtggaa tgctgtacca ctgcatcggg tgctgccaga cgatcagatc
    36481 ggcgcgagac agcgcctcct gctcggcggc aatatcgata ttgaagtcag gatagagttg
    36541 ataaagagag cgaatttcga cgccttccag cgtccttgcc tgttcaagca tccgtttatt
    36601 cgcatgggaa tgatgcggat acggatgcgc ataaattata agaatcatag gtagcccgcg
    36661 tgattacgcc tttgttttcc cacaaagtgt agtcatatat tcacgcggct tatagtcaat
    36721 aatattgatt cagaaaagag agaaatctga acaatttacc ccattctctg cccgcggatc
    36781 gtcatattgc tggtttgcgc cagtgacatc cctcgagtct ccggggcaaa cgctacggaa
    36841 atcaacaagc caaacagcga gatacccgcc cccattagca tcgtgttact gataccgtaa
    36901 ttattgataa agatcggcag tgcccaggtc gaaacaatgg tgccaatacg gcttaaggac
    36961 ataatcacgc ccacggcaga ggcgcggata tctgtcggga agagttcatt aggatagagc
    37021 cactgcaaat tacccggccc gccagagaaa aaggcataca ccgcaaaggc catcactacc
    37081 agccagatcc ccatatccgg gatcagcccc aaaaccgcca gcgccagcgt catcatggca
    37141 aagctgccaa tcaacaatgg acgccgtccg gcagtgttta gccacagcat cggcggaata
    37201 cagccgagca taaagaacag gctaatcacc acgttcccca gtgccgcgtt tttgccaacc
    37261 cccaatccca acaaaccaac gatttgcggg ccaaaggtgt aaatggcgaa cattgggatc
    37321 acctggcagg tccagatggc ggcaacaaac agaacaaaag gaaaatggcg gcgattaaac
    37381 agatcgcgaa aacgggtttg ctgcggctgc tcttcatcga aagccaccgg ttcgccaaac
    37441 agttttatca tcatctcttc gcactctttt actcgccctt tgcgtaataa ccagcgggga
    37501 gattcaggca gttcgaatcg accaatcaaa atcaacaaac aggggatcgc cgcgctaccc
    37561 agcatccagc gccagccgcc ttccacatca taaagccagt agccgaccag atcggcgcag
    37621 gtcgcgccga cataccacat ggcggcgata aagctgatgg aaaacgcccg ctgacgggta
    37681 ctggagaact cggtgatcat tgaggtggcg atgggataat ctgcaccgat gacaatgccg
    37741 ataagtaccc gcatcaccaa cagttcgaca ggggatgaga caaacatcgt cgccaccgat
    37801 atcacgccga tggcgatgat atcaatgagg aacattttgc gccgtccgac tttatcggag
    37861 atatagccaa acagcgatgt gccaacgaac agcccggcga gcgttcccgc acccagcaag
    37921 ccaatccagt cagcgtccag tttcagcgcc ggagtcagtt gctccagcgc cacgccaatc
    37981 attaccagta cataaccatc cagaaacgga ccaccacttc cccacagcaa aatgcggcgg
    38041 tgaatagagg agaatttgag atcgtcaaag tttctggacg gttgcatggg ttattcctgt
    38101 tttttgcaat gagagtcagt gcggcgttac gtatcaaacc aacatcagcc gtaacggaac
    38161 tccacaccaa aggtgccgcg cgggtattcc cactgttcca gcgccgaccc cagccccaga
    38221 atgcgacagg tgccgcactc cagacatccg gcgtaatcga agcgcacact gccgtcatcc
    38281 tgcttcttgt acagacctgc ggggcacgct ttcaccagca gctccagcac ctgtttatca
    38341 gcatcggcct tcacaacaat gtgcggatgc tcttcatcga cattgaattt attgacgccc
    38401 agtttgacgt ccacattgac gggagaagtc ataaaacggt cactcctttc atgccatcct
    38461 tgattagatt gatgaagccc actttcttac cgtggcggag gattttcttg cgcatcagtt
    38521 ctggcgcact gccatcaatg gtgaacaggt cacgcgccac gcccaccgcc agctccgggt
    38581 agccgctgaa catgcgtggg ttatcaagga acgccggtag tttctggtac atgcgcatat
    38641 cgcgcagcgg gccactctca agatgctgac gatattccgc cagtttttgc ttactgaaat
    38701 cgtcgctttt catcgctgaa agcacggttt ttgctgcagc ttccccggcg gcaatcgcca
    38761 gatccatacc gcgaatggta aaaccgaggt tcatacacat tccggcggca tcgccggcaa
    38821 tcaatacgcc gtcaccgacc agctccggca gcatgttgat gcctgcttcc ggcactacgt
    38881 gagcggaata ttccaccagc ttgccacccg cgatcagcgg tgcaacggcc ggatgttgtt
    38941 tgaaatcttc cagcatttgc ggcaccgatt tttttgcgtc atgcagatga tgcagaccac
    39001 aaaccagccc cagcgacagg gtattttcat tggtataaag gaagccgccg cccatcaggc
    39061 catcggtggg tgatcccgca aacagacaag ccgcgccctg attaccctgc aactgaaaac
    39121 ggtcttcgat taccgacttc ggtaactcga tcagttcctt cacgccaacc gccacatccg
    39181 tcggtttaac gcgttttgcc atccccaact tttcggcaag gatggaattc accccgtcag
    39241 caaggatcac cgttttcgct tcaatcacat cgccatcggc ttctacaccg acgactttgc
    39301 catcgcgctg tacgaggtta tcgacacgaa tcccggtaat taactgcgcg cccgcttctt
    39361 cggcctgctc catcaaccag gcatcaaatt tactgcgcaa aacggagtaa gaacgctggg
    39421 atggcgaggt ttcgtcaccg ttgcagtagt ccatagtcat cgctgacttt tccgtcataa
    39481 acgcgagttt ttcatgggtg atcaggcgtt ctacgggggc ggagtcggcg aaaccaggaa
    39541 taatgtgttc cagactgtgg gcatagagac gcccgccggt gacgttcttg gcacctgcgg
    39601 aattgccacg ctcgataact aacacttgcg caccttcgcg ggcgagcacc agtgcggcaa
    39661 ccgaaccggc aagccctgca ccgacgatga tggcgtcaaa gatatcttcg gacataagag
    39721 ctccctgtca gcggcagggc caaaatgcag ccctgccaga gtggatcaac gcgctaaagc
    39781 tgcggtcagc gccggaagga tcttcacggc gtcgccaaca atgccgtaat ccgcgtactg
    39841 gaagatcggc gcatttttat ctttgttgat ggcgaaaatg gtttgcgatg cattagcacc
    39901 aaccatgtgc tggatctgcc cggagatccc caccgccaag tacagttcag gtttcagcat
    39961 caggttggag ataccgacat agcgttcgtg ctccatccat ttttcgtttt ccgccaccgg
    40021 acgagaacag gccaactccg cacctatcgc cttgcaaagc tgttctgcca gcgcaatgtt
    40081 ctctttgctg ccaataccgc gaccgacgct gaccaccaga cgggctttgt cgagatcgac
    40141 gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc gccacagccg gagcctgcca
    40201 ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc tgagccgcat cgaacgtgcc
    40261 gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt tcttcgccaa tcgccagacc
    40321 accgtaaacc atgtgtttca ctgtcgcttt accgtcctgt acgctgacgg tgctggtatc
    40381 gttagacacc gccgctttaa ggcgataacc cagttttgcc gccagtaatt tgccgcgacg
    40441 ggtgtttggc agcagcacca ggccgtctgc gccgtgctgg cgaatagtgt cagccatgac
    40501 accggcgtaa tcttcgatca tccgatcgtc cggtttgccg tttaatttcc agacatgatt
    40561 agcgccgagc tggattgcct gtacgccgtc ggcatcattg aggacaaagg tgttgatttg
    40621 attagctaaa gcctgcgcac cgttcatcag ttccggcaga cgagaagggg tatcgctgaa
    40681 tacccagact tgagaaaacg tgttcatagc atcccctgta attaaatgac tttgcgaaga
    40741 ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt caatcacgat gcgctgacgt
    40801 tcgcgctgtt tcggcgcagc aacctgttgt tcagaccagg cgtccacggc gttaaaaccg
    40861 atatccgccg ccgaccatac ctggactggc tttttcgccg cgccgagaat ggctttcatc
    40921 gaaggaattt gtggggagtt gatatcagtg gaaacagcaa caaccgcagg cagcggaatg
    40981 cttaaggttt cggtttcatc ttccagttcg cgctcaacgg tgagggtatc tgccgtcagg
    41041 gagataattt tgctgacgcc gttaactgcc ggaatattga ggatttcgcc caccagcaga
    41101 ccaacctgct gggcataaag gtcggaagaa ccatcgccac agaggatcaa atcaaagcct
    41161 gctttctggg cggctgcagc cagtgcgctc gccgtttgtt gcggcagtgc ctgctcgaac
    41221 tggtcatcaa tcaccacaat cagttcatcc gagccgcgcg atagcacatc tttacgccct
    41281 ttggcgttgg tcagggcttt accgcccaca cttaaggccg tcacctgcgc ctctgctgcc
    41341 tgttgcttta gctggcaagc cgcttcaata gcgttcagat cgtattggct tattttggca
    41401 tcggctttgc tgaagtctaa tgaaccatca gcattattga ccgcaatatc ctgttcatca
    41461 ggcacgcact tatagcaagt aataatcttc attgcatctc cagaaatcat gaaggaaacg
    41521 tcgttcatat cggtggtgtt aattacagat atcacgccct ttaatttgga aataaaactt
    41581 taatcaccaa tattgaaaat gtcacacgca gataaatcca actttcaata ttgttaagtt
    41641 cctcaccaat attgaaaaca tggcgtagaa aaaagaaatt ttcaatattg ctttatggat
    41701 caccaatatt gaaagtcaga tgctattttt ttactcacaa cacaacataa caaactgatt
    41761 aataatcaaa attaacgaga aaatcgccct ctattcaccc gaaacaaaaa tgtgataaca
    41821 atcacagaat gcagcttatt gaatacccat tatgagttag ccattaacgc gtccacgagg
    41881 ttaataataa ttatattaaa tgttaacaaa aataaaacaa acgggaacgc aaaataaata
    41941 ttcgttttca cagtggaatt aactcatgaa gaatgaaaag agaaaaacgg gaatagaacc
    42001 gaaggttttc tttccgccgt taataatcgt cggcatactt tgttggctta cagtcagaga
    42061 tctggatgca gcgaatgtcg ttattaatgc tgtattcagt tacgtcacca atgtatgggg
    42121 atgggcattt gaatggtata tggtggtgat gcttttcggt tggttctggc tggtgtttgg
    42181 cccgtatgcc aaaaagcgtt taggtaacga accacctgaa tttagtaccg ccagttggat
    42241 ctttatgatg ttcgcctcct gtacgtctgc tgccgtactg ttctggggat cgattgagat
    42301 ctactactac atctccaccc cgccgtttgg cttagaaccg aactcgacag gagcgaaaga
    42361 gttggggctg gcttacagct tgttccactg gggacctctg ccgtgggcca cttacagctt
    42421 cctttcagtc gccttcgctt acttcttctt tgtccgcaaa atggaagtga ttcgccccag
    42481 ctcgacactg gtgccgctgg taggtgaaaa acacgccaaa gggttgttcg gcactatcgt
    42541 cgacaacttc tatctcgtcg ccttgatctt cgcgatgggt accagtctgg gccttgccac
    42601 gccgctggtg accgagtgta tgcaatggtt gtttggcatt ccgcataccc tgcaactgga
    42661 cgctatcatc attacctgct ggattatcct caacgccatt tgcgtcgctt gcggtctgca
    42721 aaaaggggta cgtatcgcca gtgacgtgcg tagttacctg agcttcctga tgctgggttg
    42781 ggtgttcatt gtcagcggtg ccagcttcat catgaactac ttcaccgatt cggtggggat
    42841 gttgctgatg tatctgccgc gcatgttgtt ctataccgat cccatcgcta aaggcggctt
    42901 cccgcagggc tggaccgtgt tctactgggc atggtgggtg atttatgcta tccagatgag
    42961 tatcttcctc gcccgcatct cccgtggtcg tactgtgcgt gaactgtgct tcggcatggt
    43021 gctggggctg acagcgtcaa cctggatcct gtggactgta ctcggtagta acactctgct
    43081 gttgatagat aaaaacatca tcaacattcc aaatctgatc gaacagtacg gtgtggcgcg
    43141 cgccattatc gaaacctggg ccgctctgcc actcagcacc gccaccatgt ggggcttctt
    43201 catcctctgc tttattgcca ccgttacgct ggttaacgcc tgctcttata ccctggcgat
    43261 gtccacttgc cgcgaagtac gcgatggtga agaaccaccg ctgctggtgc gtatcggttg
    43321 gtcaattctg gttggcatta tcggtattgt tttgctggcg ctcggcggcc tgaaaccgat
    43381 tcaaactgcc attatcgccg gaggatgccc gctgttcttc gtcaacatta tggtgacgct
    43441 ctcctttatt aaagacgcga aacagaactg gaaagattaa ttaaccccca aaatatcaag
    43501 aggttgaaag atggatttta atttaaatga tgagcaggaa ctgtttgtcg ccggtatccg
    43561 cgaactgatg gccagcgaaa actgggaggc ctattttgcc gagtgcgacc gtgacagcgt
    43621 ctacccggaa cgttttgtca aagcactggc ggatatgggt atcgacagtc tgctgatccc
    43681 tgaagagcac ggtggtctgg acgcggggtt tgttactctc gccgccgtgt ggatggagct
    43741 gggacgtctg ggggcaccaa cctatgtgct gtaccagttg ccgggcgggt tcaacacctt
    43801 cctgcgcgaa ggcacacaag agcagatcga caagattatg gctttccgcg gcaccggtaa
    43861 gcagatgtgg aactcagcga ttaccgaacc gggcgcgggc tccgacgtgg gtagcctgaa
    43921 aacgacttat acccgtagaa atggtaagat ttatcttaat ggtagtaagt gttttattac
    43981 cagcagcgcc tacaccccgt acatcgtggt gatggcgcgc gacggggctt ctccggacaa
    44041 acctgtctac accgaatggt ttgttgatat gagcaaaccg ggcatcaaag tgaccaaact
    44101 tgaaaagctc ggtctgcgta tggatagctg ctgtgaaatc acctttgacg acgtggaact
    44161 ggacgagaaa gacatgttcg gtcgggaagg taacggcttt aaccgcgtca aagaagagtt
    44221 cgatcatgaa cgtttcctgg tagctctcac caactacggt acggcgatgt gcgcctttga
    44281 agatgcggcg cgctacgcca accagcgcgt gcagtttggc gaggctattg gccgtttcca
    44341 gttgattcag gaaaaattcg cccacatggc gatcaaatta aactccatga aaaacatgct
    44401 gtatgaagca gcgtggaaag cagacaacgg caccatcacc tctggcgatg cagcgatgtg
    44461 caaatacttc tgcgccaatg cggcatttga agtggtggat agcgcaatgc aggtgctggg
    44521 cggtgtcggg attgcgggca accaccgcat cagccgcttc tggcgtgacc tgcgtgtaga
    44581 ccgcgtctcc gggggatctg acgaaatgca gatcctgacg ctgggtcgcg cggtgctgaa
    44641 gcaataccgc taagtccgtg cgctgcctga tgcgacgcta atgcgtctta tcaggcctac
    44701 gggagcacga atgtaggccg gataaagcgt ttacgccgca tccggcagtc atgcgctaca
    44761 taaatttttc aggagctaat aatggatcat ctacccatgc cgaaattcgg gccgttggcc
    44821 ggattgcgcg ttgtcttctc cggtatcgaa atcgccgggc cgttcgccgg gcaaatgttc
    44881 gcagaatggg gtgcggaagt tatctggatc gagaacgtcg cctgggccga caccattcgc
    44941 gttcaaccga actacccgca gctctcccgc cgcaatttgc acgcgctgtc gttaaatatt
    45001 ttcaaagatg aaggccgcga agcgtttctg aaattaatgg aaaccaccga tatcttcatc
    45061 gaagccagta aaggtccggc ctttgcccgt cgtggcatta ccgatgaagt actgtggcag
    45121 cacaacccga aactggttat cgctcacctg tccggttttg gtcagtacgg caccgaggag
    45181 tacaccaatc ttccggccta taacactatc gcccaggcct ttagtggtta cctgattcag
    45241 aacggtgatg ttgaccagcc aatgcctgcc ttcccgtata ccgccgatta cttttctggc
    45301 ctgaccgcca ccacggcggc gctggcagca ctgcataaag tgcgtgaaac cggtaaaggc
    45361 gaaagtatcg acatcgccat gtatgaagtg atgctgcgta tgggccagta cttcatgatg
    45421 gattacttca acggcggcga aatgtgcccg cgcatgagca aaggtaaaga tccctactac
    45481 gccggttgcg gtctgtataa atgcgccgac ggctacatcg tgatggaact ggtgggcatt
    45541 acccaaattg aagagtgctt taaagatatt ggcctcgcac atctactcgg tacgccggaa
    45601 atcccggaag gcactcagct tatccaccgt atcgaatgcc cttacggccc actggtagaa
    45661 gaaaaactcg atgcctggct ggcggcacat accatcgcag aagttaaaga acgctttgcc
    45721 gaactgaata tcgcctgtgc caaagtactg accgtaccgg aactggaaag caatccacag
    45781 tatgttgccc gtgaatcaat cactcagtgg caaacgatgg atggtcgcac ctgcaaaggg
    45841 ccgaacatca tgccgaaatt caaaaataac cccggacaaa tctggcgcgg aatgccctca
    45901 catggcatgg acacggctgc cattttgaaa aatatcggct acagcgaaaa cgacattcag
    45961 gagttggtca gcaaaggtct ggccaaagtt gaggactaaa cgcgtggcgt cagtcggctt
    46021 gcctgactag cgcccgccca ataacaaacg aacaaaaatg gatagaggtg caatggatat
    46081 cattggcgga caacatctac gtcaaatgtg ggacgatctt gcggacgttt acggtcataa
    46141 aacggcgctg atttgtgaat ccagcggcgg agtcgttaac cggtatagtt atcttgagtt
    46201 aaatcaggag attaaccgca cggcaaacct gttttatacg ctagggattc gcaaaggcga
    46261 caaggttgct ctacatctcg acaactgccc ggaatttatc ttttgctggt tcgggctggc
    46321 aaaaattggc gcgattatgg tgccgattaa cgcccgcctg ttgcgcgagg aaagcgcgtg
    46381 gatcctgcaa aatagccagg cgtgcctgct ggtgaccagt gcgcaattct atcctatgta
    46441 tcaacagatt cagcaggaag atgccactca attgcggcac atttgcctga cagatgtggc
    46501 acttcccgct gatgatggcg tgagttcgtt tactcaactg aaaaatcaac aacctgccac
    46561 cttgtgctat gcaccgccgc tatcgactga cgatacggcg gaaattctct tcacctccgg
    46621 caccacctcc cgaccgaaag gtgtggtgat tacccattac aacctgcgct tcgctggata
    46681 ttactccgcc tggcagtgtg cactgcgtga cgatgacgtc tacctgacgg taatgcctgc
    46741 gtttcatatc gattgccagt gtactgcggc gatggcggcg ttttctgccg gggccacctt
    46801 tgtgctggtc gagaaataca gcgcccgcgc cttctgggga caggtgcaga agtaccgcgc
    46861 caccattacc gaatgtattc cgatgatgat ccgtacgttg atggtgcagc cgccttcagc
    46921 gaacgatcgg caacaccgcc tgcgggaagt gatgttttat ctcaacttgt cggagcagga
    46981 aaaagatgcg ttttgtgaac gcttcggcgt tcgcttgctg acgtcttatg ggatgacgga
    47041 aaccattgtg ggcattatcg gcgatcgccc tggcgataaa cgacgctggc cgtcgattgg
    47101 tcgggcgggg ttttgctacg aagcggagat ccgcgacgat cacaatcgcc cgctcccggc
    47161 aggtgagatc ggtgaaatct gtattaaagg cgtaccaggg aaaaccatct tcaaagagta
    47221 ttttctcaac ccgaaagcca ctgcgaaagt gctggaagcc gacggctggc tacataccgg
    47281 cgataccgga tactgcgacg aagagggctt tttttatttc gtcgatcgcc gctgcaatat
    47341 gatcaaacgc ggtggcgaga atgtctcctg cgtggagctg gaaaatatca tagccaccca
    47401 tccaaaaatt caggatatcg tggttgtggg tattaaagat tcgattcgcg atgaagccat
    47461 caaagcattt gtggtgctga atgaaggtga aacattgagc gaagaggaat ttttccgctt
    47521 ctgcgaacaa aatatggcga aatttaaagt gccctcttat ctggagatca gaaaagatct
    47581 gccacgtaat tgctcgggga aaataattag aaagaatctg aaataaatga aacagcaggg
    47641 aactaccctg ccagctaata atcacgctat aaaacaatat gcattttttg cagggatgtt
    47701 gtcgtccctg aaaaagcaaa aatggagaaa aggaatgagt gaatcattac atctgacccg
    47761 caatggatca attctggaaa ttacccttga tcgtccaaaa gcgaatgcta ttgatgcaaa
    47821 aaccagcttt gaaatgggcg aagtatttct aaatttccgt gacgatccgc aattacgtgt
    47881 cgccattatt accggtgccg gagagaagtt cttttccgcg ggctgggatt taaaagcggc
    47941 agcagaaggc gaagcaccgg atgctgactt tggcccgggt ggatttgcgg gattaaccga
    48001 aattttcaat ctcgacaaac cggttatcgc agctgtgaac ggctatgcct ttggcggcgg
    48061 tttcgaactg gcgctggcgg cagattttat tgtttgtgcc gataacgcca gcttcgccct
    48121 gccggaagcc aagctgggca tcgttcctga cagcggcggc gtgctgcgtc taccgaagat
    48181 cctgccgcct gccatcgtca atgaaatggt gatgaccggc agacgaatgg gcgcagaaga
    48241 ggcgctgcgt tgggggatag tcaaccgcgt ggttagccag gcggaactga tggataacgc
    48301 ccgcgaactg gcgcagcagc tggttaacag cgccccgctg gcgattgcgg cgctgaaaga
    48361 gatcttccgc accaccagcg aaatgccggt agaagaagcg tatcgctata ttcgcagcgg
    48421 cgtgttgaaa cactatccgt cggttctgca ttcggaggat gccattgaag ggccgctggc
    48481 gtttgccgag aagcgcgatc cggtgtggaa aggacgttaa caaccgtgag ctattacgcc
    48541 tttgaggggt taattccggt ggttcacccg acggcgtttg tccatcccag tgccgtcttg
    48601 attggcgatg tgattgtggg agccggtgtc tacatcggcc cactcgcctc actgcgtggt
    48661 gactacgggc ggttgatcgt gcaagcggga gccaatattc aggatggctg cattatgcat
    48721 ggctactgcg acactgacac catcgttggg gaaaacggcc atatcgggca cggagcgatc
    48781 ctgcatggtt gtgtgattgg tcgcgatgca ttggtcggga tgaacagcgt gattatggat
    48841 ggcgcggtca ttggcgaaga gagcattgtt gccgccatga gctttgtcaa agcaggcttt
    48901 cacggcgaga aacgccagtt gttgatgggt acgccagccc gcgctgtacg cagtgttagt
    48961 gacgacgagt tacactggaa acggttgaat accaaagagt atcaggatct tgtcgggcgc
    49021 tgtcatgcat cgttacatga aacacagcca ctgaggcaaa tggaggaaaa tcgcccccgt
    49081 ttgcagggga cgacggatgt gacgcctaaa cggtgatctt gccggatgcg acctggttgc
    49141 tggataagat gctacaacat cgcatctggc aagcaggacg cccggtatca acgagataaa
    49201 attaacgacg catactcttt gacaacatca tctgccactt ctgctcgcgg ttaagttccg
    49261 tgagcggaac ggcaggaata cgcggcgtct ttcttacgcc gactaattta tcccggctgt
    49321 tattgcgcag gcgcgcgtaa atttgctcat cgatatcgac cacttttacc agtcgctggc
    49381 actggcatcc ccgtccttcc agcttattag ggatcatctt aacttcgcag cttatttctg
    49441 cgacttccga cagaatataa gtcagggtat taaccgcctt gctgtgttca atatcgaaat
    49501 gaatagagat ctctcttgct atcacccacc gattttcagc catcatccat tcggcgatta
    49561 acaagtagag tggtttttca acatatcctt cacacatacg aactccagtt tcacaaatta
    49621 aaagtctggc tatgactcgt tttgaagcaa ttaaacaagg ccatattaaa attgtggata
    49681 tatcgatagt atgcaacttc accgtcgata agtgtgagct aaatcctgct tatgtcatca
    49741 aaaatatcga ttccccaaaa gatttactta atgggcaaaa aaaacggtcc tcatcagaga
    49801 accgtattac ttattcgatc aaattagcgg atgaaaaata tccgccatga cacgctatta
    49861 tttgatctgc gcgtgcattt cctgcaccga aattactttt tcggtcgcgt cggcattcag
    49921 cgccatcgcg gtagcgaaac caccgttcag ggtggtgtca taatgcactt tatattgcag
    49981 cgcactgcga cggatcacgc gggagtcttc aatcgcgcga cggcctgaag tggtgttgat
    50041 gatgtaggta tattcgccat tcttgatgcg gtcctgaatg tgcggacggc cttcatgcac
    50101 cttgttcacc agacgcggat taatgcccgc ttcgcccagc acaatcgccg tgccgtgggt
    50161 cgcatccagc tcgaagccct gtttcagcag ttttgccgcc aggtccacca cgcgttcttt
    50221 atcgccttcg cgcacggaaa gcaacgcacg accgtgtttc ttcatggtgg agttgctgcc
    50281 cagctgcgct ttggcaaacg cttcagcgaa ggtgcggccc acgcccatga cttccccggt
    50341 agagcgcatt tctggcccta acagcgggtc aacacccggg aatttattga acggcagcac
    50401 cacttctttc accgagtagt acggcgggat aacttctttg gttacgccct gctcagccag
    50461 cgatttgcca gccatcacac gcgccgccac ttttgccagc ggaacgccgg tggctttgga
    50521 gacgaacgga acggtacgcg ccgcacgcgg gttaacttca atcaggtaga cttcgttgtt
    50581 tttcaccgca aactgcacgt tcatcagacc gcgcacctgc aattcgaagg ccagtttctg
    50641 cacctgctgg cgcatcacat cctgaatttc ctgacttaag gtgtaggctg gcagagaaca
    50701 tgcggagtca ccggagtgca cgcccgcctg ctcgatgtgc tccataatgc cgccaatcag
    50761 caccatttca ccgtcgcaga tggcatccac gtcaacttcc accgcgtcat cgaggaagtg
    50821 atccagcaac actggcgcat cgttagacac gctgaccgcc gtctggaagt agcgacgcag
    50881 gtcagcttcg tcatagacga tttccatcgc ccgaccgccg agaacgtaag acggacgtac
    50941 caccagcggg tagccaatct ctttcgcctt ctctaccgcc atttcaatag cggtaacggt
    51001 ggcgttcgcc ggttgtttca gtttcagacg ctcaaccgca tgctggaagc gttcacggtc
    51061 ttctgcacgg tcgatagcat ccgggctggt gccgataacc ggtacgccag cggcttccag
    51121 tgcacgcgcc agtttcagcg gggtctgacc gccgtactga acgataacgc ctttcggctt
    51181 ctcaatacgc acgatttcca gcacatcttc cagagttacc ggctcgaagt agaggcgatc
    51241 ggaggtgtcg tagtcggtag agacggtttc cgggttacag ttaaccataa tggtctcaaa
    51301 accgtcttca cgcagcgcca gcgaggcgtg tacgcagcag tagtcgaatt cgataccctg
    51361 accgatacgg tttggaccgc cgccaagcac catgattttt tcacggtcgg tagacggatt
    51421 cgcttcgcac tcttcttcat aagtggagta catgtaagcg gtgtcggtgg caaactctgc
    51481 cgcacaggta tccacacgct tgtagaccgg gtgcaggtca tattggtcac gcagcttacg
    51541 gatttccgct tcgcgtacac ccgcgagttt tgccagacgc gcatcggcaa agcctttgcg
    51601 tttcagctgg cgcaggaagt cagcgttcag gccagtgatg cccacttccg ccactttctc
    51661 ttccagacgc accagttctt caatctgtac caggaaccag cggtcaatgt tggtcaggtt
    51721 gaagacgccg tccacagaca ggcccgcacg gaaagcatcg gcgatgtacc agatacgctc
    51781 tgcgcctgcg tctttcagtt cgcgacggat tttggttaac gcttccgggt catcgaggct
    51841 cactttcggg tcgaatccag tcgcaccgac ttccaggccg cgcagcgctt tttgcaggga
    51901 ttcctgctgc gtgcgaccaa tcgccatcac ttcgccaacc gatttcatct gagtggtcag
    51961 acggtcgtta gcaccagcga atttttcgaa gttgaagcga ggaattttag taaccacata
    52021 gtcgatggac ggctcgaagg aggccggagt acgtccgcca gtgatgtcgt tcatcagttc
    52081 gtcgagggtg taacccaccg ccagtttcgc cgccacttta gcaatcggga aaccggtcgc
    52141 tttcgacgcc agcgccgaag aacgggacac gcgtgggttc atttcgataa caatcaggcg
    52201 accgtttttc gggttcaccg caaactggac attggaacca ccggtttcaa cgccgatttc
    52261 acgcagcacc gccatcgagg cgttacgcat gatttgatat tctttgtcgg tcagcgtttg
    52321 ggctggcgcg acagtgatgg agtcaccggt atggatgccc atcgcatcaa agttttcgat
    52381 agagcagacg atgatgcagt tgtcgttttt atcacgcacc acttccatct cgtactcttt
    52441 ccagccgatc agcgactcat caatcagcag ctctttggtt ggggagagat ccagaccgcg
    52501 ggcgcaaatt tcttcaaact cttcgcggtt ataagcgata ccgccgccgc taccgcccat
    52561 ggtaaaggat gggcgaataa tgcacgggaa gcccacgtca gcggcaaccg ccagcgcttc
    52621 ttccatcgtg tgtgcgatac cggaacgcgc ggtttccaga ccaattttct tcatcgctac
    52681 gtcgaaacga cggcggtctt ctgctttatc aatcgcatcg gcggtcgcac caatcatagt
    52741 cacgccgaac tcttccaaca cgccctgacg ctccagctcc agcgcgcagt tcagcgccgt
    52801 ctgaccgccc atcgttggca gcaccgcgtc cgggcgctct ttttcaataa tcttgcgtac
    52861 tacttcccag tgaatcggct cgatgtaggt cgcatcggcc atttccgggt cggtcatgat
    52921 ggtcgccggg ttggagttca ccagaatgac gcggtaaccc tcttcgcgca gggctttaca
    52981 tgcttgcgcg ccagagtagt caaactcaca cgcctgaccg ataacaatcg ggcccgcacc
    53041 cagaatcagg atacttttta tatctgtacg ttttggcatg gctcttttac tcctgattac
    53101 ttagcggttt tacggtactg ctcaattaac tcgataaagt ggtcgaacaa cggcgctgcg
    53161 tcgtgtggcc cagggctggc ttccgggtga ccctggaagc tgaacgccgg tttatcagtg
    53221 cgatgaatgc cctgtaacgt accgtcgaac agggatttat gcgtgacacg caggtttgca
    53281 ggtaatgttg cttcatccac cgcaaaaccg tggttctggg cggtgatcat caccacgttt
    53341 ttctccacat ctttaaccgg atggttgccg ccgtggtgac caaatttcat tttgacagtc
    53401 ttcgcaccgc tcgccagcgc cagcagctga tgaccgaggc agatgccgaa taccggaata
    53461 tcggtttcga ggaatttctg gatggcggta atggcgtaat cgcacggggc cgggtcgcca
    53521 ggaccgttgg agaggaagat gccgtctgga ttcattttca gcacatcttc cgcagaagtt
    53581 tgcgccggaa cgatggtcag gcgacagcct ctatccacca gcatccgcag gatgttgcgc
    53641 ttggcaccaa aatcataagc cacgacgtgg aacggcagct cgtcttcttt tttcgcttct
    53701 ggcaggccgc cggtcaacgt ccagctccct tgtgtccagc tataggcttc tgcggtggtc
    53761 acttcttttg ccagatccat gccattcaga cctgggaacg cgcgggcttt ttctaacgcc
    53821 aacgccgcat ccgggttatc gcctgcgata atgcagccat tctgtgcgcc tttctcgcgc
    53881 agtaaacgcg tcagcttacg ggtatcgata tcggcaatcg ccacgatgtt atggcgcttc
    53941 aggtaagaag aaaggtcttc ggtattacgg aagttgctgg caatcagcgg cagatcgcga
    54001 atcaccagac cttgtgcatg tacctgagaa gattcttcat cggcgtcatt agtgccgaca
    54061 ttgccaatat ggggataagt aagagtaacg atttgacgag aataggaagg atcagtgagg
    54121 atttcttgat aaccggtcat tgaagtattg aaaacgactt ccccaaccgc cgaacctgtt
    54181 gcccctatgg cccgaccgtg aaactgggtt ccgtcttcca gaaccaatag cgctgactta
    54241 atcaaaacac cctccagaga atattcactc actttatttg catattaatt cacaatgatg
    54301 acgtaaatca atgcaaatct gcttaccgac gaatttctgg caaacggcgg cattctggag
    54361 atatgggcgc taaaagtcaa cttaatggcg ggatttttta tattattttg tggctcatgc
    54421 atgttttaca ggcattaagc gacaaaaaga tcatataacc tgttctggaa aacgcttgcg
    54481 cagcaagaag attgcatgaa ataaaagcga gcagggaaaa gtggaccaaa tggtcaaaat
    54541 ttacattaaa acaacaaaaa ccgaagacaa aacatgtaat ttttggcact tataga
//
LOCUS       DAZGAN020000020        52844 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGAN020000020 DAZGAN020000000
VERSION     DAZGAN020000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 52844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.020"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1264
                     /gene="uidC"
                     /locus_tag="ACHKYO_RS06895"
                     /old_locus_tag="pgaptmp_001379"
     CDS             <1..1264
                     /gene="uidC"
                     /locus_tag="ACHKYO_RS06895"
                     /old_locus_tag="pgaptmp_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HGU4428250.1"
                     /translation="RKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRAD
                     KPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGDK
                     SFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALGY
                     WALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDTS
                     RYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETAL
                     VSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVNY
                     RLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKGR
                     EKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(1309..2817)
                     /gene="ydgA"
                     /locus_tag="ACHKYO_RS06900"
                     /old_locus_tag="pgaptmp_001380"
     CDS             complement(1309..2817)
                     /gene="ydgA"
                     /locus_tag="ACHKYO_RS06900"
                     /old_locus_tag="pgaptmp_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU4428251.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(2918..4093)
                     /gene="manA"
                     /locus_tag="ACHKYO_RS06905"
                     /old_locus_tag="pgaptmp_001381"
     CDS             complement(2918..4093)
                     /gene="manA"
                     /locus_tag="ACHKYO_RS06905"
                     /old_locus_tag="pgaptmp_001381"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HGU4428252.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            4292..5938
                     /gene="fumB"
                     /locus_tag="ACHKYO_RS06910"
                     /old_locus_tag="pgaptmp_001382"
     CDS             4292..5938
                     /gene="fumB"
                     /locus_tag="ACHKYO_RS06910"
                     /old_locus_tag="pgaptmp_001382"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU4428253.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAL
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            6081..7484
                     /gene="fumC"
                     /locus_tag="ACHKYO_RS06915"
                     /old_locus_tag="pgaptmp_001383"
     CDS             6081..7484
                     /gene="fumC"
                     /locus_tag="ACHKYO_RS06915"
                     /old_locus_tag="pgaptmp_001383"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HGU4428254.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGSRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(7481..8410)
                     /gene="tus"
                     /locus_tag="ACHKYO_RS06920"
                     /old_locus_tag="pgaptmp_001384"
     CDS             complement(7481..8410)
                     /gene="tus"
                     /locus_tag="ACHKYO_RS06920"
                     /old_locus_tag="pgaptmp_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HGU4428255.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(8486..9787)
                     /gene="rstB"
                     /locus_tag="ACHKYO_RS06925"
                     /old_locus_tag="pgaptmp_001385"
     CDS             complement(8486..9787)
                     /gene="rstB"
                     /locus_tag="ACHKYO_RS06925"
                     /old_locus_tag="pgaptmp_001385"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HGU4428256.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSP"
     gene            complement(9791..10510)
                     /gene="rstA"
                     /locus_tag="ACHKYO_RS06930"
                     /old_locus_tag="pgaptmp_001386"
     CDS             complement(9791..10510)
                     /gene="rstA"
                     /locus_tag="ACHKYO_RS06930"
                     /old_locus_tag="pgaptmp_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HGU4428257.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            10639..10974
                     /gene="ydgC"
                     /locus_tag="ACHKYO_RS06935"
                     /old_locus_tag="pgaptmp_001387"
     CDS             10639..10974
                     /gene="ydgC"
                     /locus_tag="ACHKYO_RS06935"
                     /old_locus_tag="pgaptmp_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HGU4428258.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(10971..11693)
                     /gene="folM"
                     /locus_tag="ACHKYO_RS06940"
                     /old_locus_tag="pgaptmp_001388"
     CDS             complement(10971..11693)
                     /gene="folM"
                     /locus_tag="ACHKYO_RS06940"
                     /old_locus_tag="pgaptmp_001388"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HGU4428259.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(11730..13112)
                     /gene="ydgI"
                     /locus_tag="ACHKYO_RS06945"
                     /old_locus_tag="pgaptmp_001389"
     CDS             complement(11730..13112)
                     /gene="ydgI"
                     /locus_tag="ACHKYO_RS06945"
                     /old_locus_tag="pgaptmp_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4428260.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(13298..14242)
                     /gene="ydgH"
                     /locus_tag="ACHKYO_RS06950"
                     /old_locus_tag="pgaptmp_001390"
     CDS             complement(13298..14242)
                     /gene="ydgH"
                     /locus_tag="ACHKYO_RS06950"
                     /old_locus_tag="pgaptmp_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HGU4428261.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            14766..16298
                     /gene="pntA"
                     /locus_tag="ACHKYO_RS06955"
                     /old_locus_tag="pgaptmp_001391"
     CDS             14766..16298
                     /gene="pntA"
                     /locus_tag="ACHKYO_RS06955"
                     /old_locus_tag="pgaptmp_001391"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HGU4428262.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            16309..17697
                     /gene="pntB"
                     /locus_tag="ACHKYO_RS06960"
                     /old_locus_tag="pgaptmp_001392"
     CDS             16309..17697
                     /gene="pntB"
                     /locus_tag="ACHKYO_RS06960"
                     /old_locus_tag="pgaptmp_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HGU4428263.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTQMLFGD
                     AKASVDAILKAL"
     gene            complement(17722..18756)
                     /gene="tqsA"
                     /locus_tag="ACHKYO_RS06965"
                     /old_locus_tag="pgaptmp_001393"
     CDS             complement(17722..18756)
                     /gene="tqsA"
                     /locus_tag="ACHKYO_RS06965"
                     /old_locus_tag="pgaptmp_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HGU4428264.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            19168..19533
                     /gene="mdtJ"
                     /locus_tag="ACHKYO_RS06970"
                     /old_locus_tag="pgaptmp_001394"
     CDS             19168..19533
                     /gene="mdtJ"
                     /locus_tag="ACHKYO_RS06970"
                     /old_locus_tag="pgaptmp_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HGU4428265.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            19520..19798
                     /gene="mdtI"
                     /locus_tag="ACHKYO_RS06975"
                     /old_locus_tag="pgaptmp_001395"
     CDS             19520..19798
                     /gene="mdtI"
                     /locus_tag="ACHKYO_RS06975"
                     /old_locus_tag="pgaptmp_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HGU4428266.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKG"
     gene            complement(19888..20709)
                     /gene="ydgD"
                     /locus_tag="ACHKYO_RS06980"
                     /old_locus_tag="pgaptmp_001396"
     CDS             complement(19888..20709)
                     /gene="ydgD"
                     /locus_tag="ACHKYO_RS06980"
                     /old_locus_tag="pgaptmp_001396"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HGU4428267.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(20809..20892)
                     /locus_tag="ACHKYO_RS06985"
                     /old_locus_tag="pgaptmp_001397"
     CDS             complement(20809..20892)
                     /locus_tag="ACHKYO_RS06985"
                     /old_locus_tag="pgaptmp_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4428268.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(20985..21293)
                     /gene="asr"
                     /locus_tag="ACHKYO_RS06990"
                     /old_locus_tag="pgaptmp_001398"
     CDS             complement(20985..21293)
                     /gene="asr"
                     /locus_tag="ACHKYO_RS06990"
                     /old_locus_tag="pgaptmp_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HGU4428269.1"
                     /translation="MKKVLGLVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            21564..21668
                     /locus_tag="ACHKYO_RS06995"
                     /old_locus_tag="pgaptmp_001399"
     CDS             21564..21668
                     /locus_tag="ACHKYO_RS06995"
                     /old_locus_tag="pgaptmp_001399"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HGU4428270.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(21717..22970)
                     /gene="ynfM"
                     /locus_tag="ACHKYO_RS07000"
                     /old_locus_tag="pgaptmp_001400"
     CDS             complement(21717..22970)
                     /gene="ynfM"
                     /locus_tag="ACHKYO_RS07000"
                     /old_locus_tag="pgaptmp_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4428271.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            23077..23970
                     /gene="ynfL"
                     /locus_tag="ACHKYO_RS07005"
                     /old_locus_tag="pgaptmp_001401"
     CDS             23077..23970
                     /gene="ynfL"
                     /locus_tag="ACHKYO_RS07005"
                     /old_locus_tag="pgaptmp_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4428272.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            24105..25325
                     /gene="mlc"
                     /locus_tag="ACHKYO_RS07010"
                     /old_locus_tag="pgaptmp_001402"
                     /gene_synonym="dgsA"
     CDS             24105..25325
                     /gene="mlc"
                     /locus_tag="ACHKYO_RS07010"
                     /old_locus_tag="pgaptmp_001402"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HGU4428273.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            25450..26145
                     /gene="bioD"
                     /locus_tag="ACHKYO_RS07015"
                     /old_locus_tag="pgaptmp_001403"
     CDS             25450..26145
                     /gene="bioD"
                     /locus_tag="ACHKYO_RS07015"
                     /old_locus_tag="pgaptmp_001403"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU4428274.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(26098..27390)
                     /gene="clcB"
                     /locus_tag="ACHKYO_RS07020"
                     /old_locus_tag="pgaptmp_001404"
     CDS             complement(26098..27390)
                     /gene="clcB"
                     /locus_tag="ACHKYO_RS07020"
                     /old_locus_tag="pgaptmp_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HGU4428275.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVGLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(27549..28163)
                     /gene="dmsD"
                     /locus_tag="ACHKYO_RS07025"
                     /old_locus_tag="pgaptmp_001405"
     CDS             complement(27549..28163)
                     /gene="dmsD"
                     /locus_tag="ACHKYO_RS07025"
                     /old_locus_tag="pgaptmp_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HGU4428276.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(28206..29060)
                     /gene="ynfH"
                     /locus_tag="ACHKYO_RS07030"
                     /old_locus_tag="pgaptmp_001406"
     CDS             complement(28206..29060)
                     /gene="ynfH"
                     /locus_tag="ACHKYO_RS07030"
                     /old_locus_tag="pgaptmp_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HGU4428277.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRSREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(29062..29679)
                     /gene="ynfG"
                     /locus_tag="ACHKYO_RS07035"
                     /old_locus_tag="pgaptmp_001407"
     CDS             complement(29062..29679)
                     /gene="ynfG"
                     /locus_tag="ACHKYO_RS07035"
                     /old_locus_tag="pgaptmp_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HGU4428278.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(29690..32113)
                     /gene="ynfF"
                     /locus_tag="ACHKYO_RS07040"
                     /old_locus_tag="pgaptmp_001408"
     CDS             complement(29690..32113)
                     /gene="ynfF"
                     /locus_tag="ACHKYO_RS07040"
                     /old_locus_tag="pgaptmp_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HGU4428279.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(32174..34599)
                     /gene="ynfE"
                     /locus_tag="ACHKYO_RS07045"
                     /old_locus_tag="pgaptmp_001409"
                     /pseudo
     CDS             complement(32174..34599)
                     /gene="ynfE"
                     /locus_tag="ACHKYO_RS07045"
                     /old_locus_tag="pgaptmp_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
     gene            complement(34798..35103)
                     /gene="ynfD"
                     /locus_tag="ACHKYO_RS07050"
                     /old_locus_tag="pgaptmp_001410"
     CDS             complement(34798..35103)
                     /gene="ynfD"
                     /locus_tag="ACHKYO_RS07050"
                     /old_locus_tag="pgaptmp_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HGU4428280.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            35211..35921
                     /gene="ynfC"
                     /locus_tag="ACHKYO_RS07055"
                     /old_locus_tag="pgaptmp_001411"
     CDS             35211..35921
                     /gene="ynfC"
                     /locus_tag="ACHKYO_RS07055"
                     /old_locus_tag="pgaptmp_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HGU4428281.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(35924..36484)
                     /gene="speG"
                     /locus_tag="ACHKYO_RS07060"
                     /old_locus_tag="pgaptmp_001412"
     CDS             complement(35924..36484)
                     /gene="speG"
                     /locus_tag="ACHKYO_RS07060"
                     /old_locus_tag="pgaptmp_001412"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HGU4428282.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(36519..36860)
                     /gene="ynfB"
                     /locus_tag="ACHKYO_RS07065"
                     /old_locus_tag="pgaptmp_001413"
     CDS             complement(36519..36860)
                     /gene="ynfB"
                     /locus_tag="ACHKYO_RS07065"
                     /old_locus_tag="pgaptmp_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HGU4428283.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            36995..37321
                     /gene="ynfA"
                     /locus_tag="ACHKYO_RS07070"
                     /old_locus_tag="pgaptmp_001414"
     CDS             36995..37321
                     /gene="ynfA"
                     /locus_tag="ACHKYO_RS07070"
                     /old_locus_tag="pgaptmp_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HGU4428284.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            37527..38741
                     /gene="rspA"
                     /locus_tag="ACHKYO_RS07075"
                     /old_locus_tag="pgaptmp_001415"
     CDS             37527..38741
                     /gene="rspA"
                     /locus_tag="ACHKYO_RS07075"
                     /old_locus_tag="pgaptmp_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HGU4428285.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            38753..39772
                     /gene="rspB"
                     /locus_tag="ACHKYO_RS07080"
                     /old_locus_tag="pgaptmp_001416"
     CDS             38753..39772
                     /gene="rspB"
                     /locus_tag="ACHKYO_RS07080"
                     /old_locus_tag="pgaptmp_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HGU4428286.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADRAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(39960..41240)
                     /locus_tag="ACHKYO_RS07085"
                     /old_locus_tag="pgaptmp_001417"
     CDS             complement(39960..41240)
                     /locus_tag="ACHKYO_RS07085"
                     /old_locus_tag="pgaptmp_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HGU4428287.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEARKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(41275..41526)
                     /locus_tag="ACHKYO_RS07090"
                     /old_locus_tag="pgaptmp_001418"
     CDS             complement(41275..41526)
                     /locus_tag="ACHKYO_RS07090"
                     /old_locus_tag="pgaptmp_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4428288.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            complement(41599..44076)
                     /locus_tag="ACHKYO_RS07095"
                     /old_locus_tag="pgaptmp_001419"
     CDS             complement(41599..44076)
                     /locus_tag="ACHKYO_RS07095"
                     /old_locus_tag="pgaptmp_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4428289.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVAVATAIEAGDERRARAKFHW
                     QFLEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESD
                     PMNVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMP
                     EVNAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLA
                     RAKANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHR
                     WAKKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYIC
                     EYLTTKGVFENEETDPGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPF
                     YFLFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDS
                     EQPEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRD
                     SVNQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPD
                     CGAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHDSDNETGETAD
                     RSIKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKT
                     IDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQV
                     WGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERH
                     NALDDARYQAKYVSVIWQKLIPSQADS"
     gene            complement(44170..44361)
                     /gene="ydfD"
                     /locus_tag="ACHKYO_RS07100"
                     /old_locus_tag="pgaptmp_001420"
     CDS             complement(44170..44361)
                     /gene="ydfD"
                     /locus_tag="ACHKYO_RS07100"
                     /old_locus_tag="pgaptmp_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HGU4428290.1"
                     /translation="MNSAFALVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(44358..44546)
                     /gene="dicB"
                     /locus_tag="ACHKYO_RS07105"
                     /old_locus_tag="pgaptmp_001421"
                     /gene_synonym="ftsT"
     CDS             complement(44358..44546)
                     /gene="dicB"
                     /locus_tag="ACHKYO_RS07105"
                     /old_locus_tag="pgaptmp_001421"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4428291.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            complement(45507..45608)
                     /gene="ydfB"
                     /locus_tag="ACHKYO_RS07110"
                     /old_locus_tag="pgaptmp_001422"
                     /pseudo
     CDS             complement(45507..45608)
                     /gene="ydfB"
                     /locus_tag="ACHKYO_RS07110"
                     /old_locus_tag="pgaptmp_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
     gene            complement(45610..45765)
                     /gene="ydfA"
                     /locus_tag="ACHKYO_RS07115"
                     /old_locus_tag="pgaptmp_001423"
     CDS             complement(45610..45765)
                     /gene="ydfA"
                     /locus_tag="ACHKYO_RS07115"
                     /old_locus_tag="pgaptmp_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4428292.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(45932..46339)
                     /gene="dicA"
                     /locus_tag="ACHKYO_RS07120"
                     /old_locus_tag="pgaptmp_001424"
     CDS             complement(45932..46339)
                     /gene="dicA"
                     /locus_tag="ACHKYO_RS07120"
                     /old_locus_tag="pgaptmp_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional dual regulator DicA"
                     /protein_id="HGU4428293.1"
                     /translation="MDTKNLTIGERIRYRRKNLKHTQRSLAKALKISHVSVSQWERDD
                     SEPTGKNLFALSKVLQCSPTWILFGDEDKQPTPPVEKPVALAPKELELLELFNALPES
                     EQDTQLAEMRARVKNFNKLFEELLKARQRTNKR"
     gene            46423..46653
                     /gene="dicC"
                     /locus_tag="ACHKYO_RS07125"
                     /old_locus_tag="pgaptmp_001425"
     CDS             46423..46653
                     /gene="dicC"
                     /locus_tag="ACHKYO_RS07125"
                     /old_locus_tag="pgaptmp_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicB transcriptional regulator DicC"
                     /protein_id="HGU4428294.1"
                     /translation="MLKTDALLYFGSKTKLAQAAGIRLASLYSWKGDLVPEGRAMRLQ
                     EASGGELQYDPKVYDEYRKTKRAGRLNNENHS"
     gene            46637..47158
                     /locus_tag="ACHKYO_RS07130"
                     /old_locus_tag="pgaptmp_001426"
     CDS             46637..47158
                     /locus_tag="ACHKYO_RS07130"
                     /old_locus_tag="pgaptmp_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4428295.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERSNID
                     VQRVTYEGGAVDQRALSVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRISDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYESSFC"
     gene            47139..48104
                     /locus_tag="ACHKYO_RS07135"
                     /old_locus_tag="pgaptmp_001427"
     CDS             47139..48104
                     /locus_tag="ACHKYO_RS07135"
                     /old_locus_tag="pgaptmp_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4428296.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLNESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTTEQDFLTRNPDAVVFSVKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPEQRCVNHISEPDTEIPPGFRG"
     gene            48145..48546
                     /locus_tag="ACHKYO_RS07140"
                     /old_locus_tag="pgaptmp_001428"
     CDS             48145..48546
                     /locus_tag="ACHKYO_RS07140"
                     /old_locus_tag="pgaptmp_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4428297.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKTAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRMSEVMQRILAFYQG"
     gene            complement(48849..49595)
                     /locus_tag="ACHKYO_RS07145"
                     /old_locus_tag="pgaptmp_001429"
     CDS             complement(48849..49595)
                     /locus_tag="ACHKYO_RS07145"
                     /old_locus_tag="pgaptmp_001429"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGU4428298.1"
                     /translation="MNFQNKIAVITGSTTGIGEAVAEQLHKHGAKVVIVSRSSEQAKQ
                     KAEQLSSQGQQAMGIGCDVSQPEQVRQMIDEVIKHFGRLDYAVNNAGLTGEHGKNITE
                     QTVENWDKVIATSLSGVFHCLKYEIPQMMKFGGSIVNLSAVNGLVGIPGLAPYTVAKH
                     GIIGLTQTAALEFACQGIRINAVAPGYVQTQRMSEFPENIVSSFANSHPMKRMAKMQE
                     IADFILFLLSDSSTFCTGGVYPIDGGYLAE"
     gene            complement(49667..50356)
                     /locus_tag="ACHKYO_RS07150"
                     /old_locus_tag="pgaptmp_001430"
     CDS             complement(49667..50356)
                     /locus_tag="ACHKYO_RS07150"
                     /old_locus_tag="pgaptmp_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4428299.1"
                     /translation="MTRTERLLELLQILRAQRYPITASILSERLGISVRSLYRDIKTL
                     QHQGVCIEGGAGIGYIVKSDFHLPPLNLSHEEINAITLGLNWVSHNTDYNFKITAKNA
                     LAKIHAVIPSELKNLIESQSYLIGPSENNEIFFEDIRNAIKKRKKIKINYCDKKDSYT
                     SRVIWPIGLVYMESCWLLVAWCEMRNDFRHFRTDKIEDIVQLDSNYSESRTILLKKWR
                     IKEGICEGKEY"
     gene            50575..51177
                     /locus_tag="ACHKYO_RS07155"
                     /old_locus_tag="pgaptmp_001431"
     CDS             50575..51177
                     /locus_tag="ACHKYO_RS07155"
                     /old_locus_tag="pgaptmp_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGU4428300.1"
                     /translation="MNTSPWNKDRIIGQKRPLQISHIWGIRIRLELEGKTRDLALFNM
                     ALDSKLRGCDLVKLKVSDVAYGSSVSSRATVLQQKTGSPVQFEITKGTREAVAALIKL
                     GNLHSKDFLFRSRVGTNQHISTRQYNRIFHGWIEKLGLEGSLYSTHSMRRTKPYLIYK
                     KTKNLRVIQLLLGHKKLESTVRYLGIEVDDALEISESIEV"
     gene            51329..52450
                     /locus_tag="ACHKYO_RS07160"
                     /old_locus_tag="pgaptmp_001432"
     CDS             51329..52450
                     /locus_tag="ACHKYO_RS07160"
                     /old_locus_tag="pgaptmp_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4428301.1"
                     /translation="MAITVYSRTFKKELDMVQLLNHYSSDNALNFSDFKEFVAFDAEC
                     PICNVSGAIVVSEGYSKRTNKIVSQSHFAFRTKDGTDAHKVFCDHYSGDDKQIDSSRD
                     GFIKFGTSGSEITSIIRELVCRGIEHDEFNQEDIRNMRQWFTELRQSGTFSVNYSPHM
                     INLIRASMYSRKGESKYIVDEERKNKSWFDINDEVYRSLRFKYPSLMIDMTNENNSVF
                     YNLVNSTTFTKQAHRLVARDRGYQVYDRRLLKEKYEATIRVAQKITRHHEFLFRKIRG
                     ISAVKKNNPLLAVSALLLFVSDWNETTAINKFIKLCGVGKSKNNDEGNVIGLNPFIHY
                     DVWNVIHQLKDLMVTLPDFSNLDEEFDREKSRLIELYKL"
BASE COUNT        13460 a        12452 c        13120 g        13812 t
ORIGIN      
        1 gagaaaaata gtggccatgg ccgttatttg cctgacggct gcctctggcc ttacctctgc
       61 ttatgcggcg caactggctg acgatgaagc gggactacgc atcagactga aaaacgaatt
      121 gcgcagggcg gataagccca gtgctggcgc gggaagagat atttacgcat gggtacaggg
      181 aggattgctc gatttcaata gtggttatta ttccaatatt attggcgttg aaggcggggc
      241 atattatgtt tataaattag gtgctcgtgc tgatatgagt acccggtggt atcttgatgg
      301 tgataaaagt tttggctttg ccctgggggc agtaaaaata aaacccagtg aaaatagcct
      361 gcttaaatta ggtcgcttcg ggacggatta tagttatggt agcttacctt atcgtattcc
      421 gctaatggct ggcagttcgc aacgtacatt accgacagtt tctgaaggag cattaggtta
      481 ttgggcttta acaccaaata ttgatctgtg gggaatgtgg cgttcacgag tatttttatg
      541 gactgattca acaaccggta ttcgtgatga aggggtgtat aacagccaga cgggaaaata
      601 cgataaacat cgcgcacgtt cttttttagc cgccagttgg catgatgata ccagtcgcta
      661 ttctctgggg gcatcggtac agaaagatgt ttccaatcag atacaaagta ttctcgagaa
      721 aagcataccg ctcgacccga attatacgtt gaaaggggag ttgctcggct tttacgcgca
      781 gctcgaaggt ttaagtcgta ataccagcca gcccaatgaa acggcgttgg ttagtggaca
      841 attgacctgg aatgcgccgt ggggaagcgt atttggcagt ggtggttatt tgcgccatgc
      901 aatgaatggt gccgtggtgg ataccgacat tggctatccc ttttcattaa gtcttgatcg
      961 taaccgtgaa ggaatgcagt cctggcaatt gggcgtcaac tatcgtttaa cgccgcaatt
     1021 tacgctgaca tttgcaccga ttgtgactcg cggctatgaa tccagtaaac gagatgtgcg
     1081 gattgaaggc acgggtatct taggtggtat gaactatcgg gtcagcgaag ggccgttaca
     1141 agggatgaat ttctttcttg ctgccgataa agggcgggaa aagcgcgatg gcagtacgct
     1201 gggcgatcgc ctgaattact gggatgtgaa aatgagtatt cagtatgact ttatgctgaa
     1261 gtaaaaaata acgccggaga gaaaaatctc cggcgttttg tctgtgaatt actgctgcgg
     1321 aatagcgggt acgaccggaa catttaatgc cggcattgca aacataccaa cgaaatcttc
     1381 cagtggcatt ttctgcccgt ttaacgttat ctgaccgtta gtatattgca ggctggtggt
     1441 gatggtattg tcctgcaagg tggtcagacg gaacatctgc cccattgctg atgcaccttc
     1501 aacttgctgt ttcgccagtt ttttcgcttg atcttcctga taaccttcca gcttcgctac
     1561 ctgagtcata aactcagttg ccatatccac cggaatggtc agtttcgcat ccagagattt
     1621 aaccgaacga tctacctcct gcgccagcgt ttgcggcgct tctttagtcg ttgccggatc
     1681 tttcaggaac agcgacagat tcagcgcact ttcaccctga ctgtttttcc agcttagcgg
     1741 cgcgatagta atcaccggat cgcctttcaa catcagcggc agggcgctaa agaaggcttc
     1801 cgtcactttc tcctgataaa gttcgggatt gttggcaatt tctggctgtg ccagcagcgc
     1861 ctgagtttgc gcgttatatt gctggctaaa ctgatgccag gcttcaccat caatctggcc
     1921 gacttttaaa gtcagcttgc cgctgcccag atcctgattc tgtaccttca ggctgtttag
     1981 cgagtaatcc agttggctat tgatcgtttt accgtcattg accagatccg atttaccgct
     2041 gatctccatg ccttccagca gtgccagttc tttgccttcc actgaaatgg tcattttttc
     2101 cagtgacagt ttttggtttc ctacgcgctc accaaaactt gccagcgtgc tggaaccgtc
     2161 ggttttcaga ttattaaagg tcaactgcac tttctggtta tattcgttaa ctgcgtctat
     2221 ccgaccactt tgcgcctccc cggaaaggga gatggctttg ccgtctctgt cagcatttaa
     2281 ctggaactcg ccgccgctaa aggcgacttt ttcatccttt tgctcgtaat tcagtggctt
     2341 gagcgaaata tcggaactgg aatcaccgct gtaaccaatg cgcgagttaa tctcaaaagg
     2401 cgtttcacct tttgccatat caaacagtgg tttgcttact tcgttattaa ccagcgtggt
     2461 ttgaattgat gccatcgacg ggatcaggtt cagtttttta agctgggcaa gcgggaaggg
     2521 accatgatca accgattcgt tgaagatgac gctctgaccg cttttaatcc acggattttc
     2581 tttcccggca atgggtttca ccaacagttg caactggctg ctgaatacgc cgcgatgata
     2641 gttttgataa ctcacttcca ggttggattc aggagctgtc agtttgagtt gcgcgttcgc
     2701 ctgcgcgacc atgtcttcga gatgggtttc aatcttcttg cctgtatacc atgcgccgcc
     2761 tgtccagact acgcctagcg caacaatgac gcctaccgct accagcgatt tattcataat
     2821 gattatccat aaaatgaaat caggcggact ggccgcctga aggtgttata agcctttaat
     2881 aagcttagca agagatgtta attttttcag taagctctta cagcttgttg taaacacgcg
     2941 ctaaacggcc gtggcctttg acagtcaccg gtgattcgtt ggcggcaata aacgctgatt
     3001 cacccggttt aagctgtaac tgctgagaac ctttccacaa cgttgcatcg ccttcgacgc
     3061 agaacaaaat ggcggcactc tgctggctaa tggtggtttc tttatcacta aggtcatgca
     3121 gcgagaaggc aaaatcatcc actggaatcg ggaagtccag ttctgcacct tgtttcaccg
     3181 gctgggtcaa caactggtta gccggtttgg cttcgaattt cacattggca accagttccg
     3241 gaatatcaat gtacttaggc gtcagacccg cacgcagcac gttatcggag ttcgccatca
     3301 cttccagcgc cacgccttgc aggtaagcgt gcggtgtttc agcgaacagg aacatcgctt
     3361 cgccaggatt caatttcacc acattcagta acagcgggga gaacaggccg ctgtcttccg
     3421 ggtaaaattc agaaattaaa cgaatcgttt gccacggttc accctgctga ctgtctagcg
     3481 ccgattttaa aatcgccagc gcgcgggatt tttcttcacc ctgcatattc aacaggctgg
     3541 cgaacagttc gcttaaacgt tcggcattag gctgttgtaa aaagtgagca attgccggat
     3601 gtgcacctgc gactggctgg agtagggaga caatctcgga aaactcacga aacgcgttca
     3661 tcgcaaggaa aggcgtcagc gcaaaaacca gctccggctt gtggttagga tctttatagt
     3721 tacgctcggc ggcatccatc gggatacctg cggcattttc tttggcaaaa ccgatttcag
     3781 aattgcgttt gtttggatga acctgaatgg agagcggctg tgctgcgcat aatactttga
     3841 acaggaaagg cagttcgcca aagcgtttgg caacggcctc tccgagcaga gtcgatttat
     3901 cactctcaat cacatcacgc agtgaaacga tatctccggc ggcattctgc actcgtgaac
     3961 tgcttttcgg atgtgcgccc atccacagct cggccatcgg ctggctggac ggattttcca
     4021 taccataaag ttcagtcaac gccgttttgc tgccccaggc atagttttgc actgagttaa
     4081 tgagtttttg catgatcaat ccctgtttta atgtggaaat taatcccact attaaagcaa
     4141 gaatcctacg ggaagtaacc tggagccgca aaaagtcgta ctagtctcag tttttgttaa
     4201 aaaagtgtgt aggatattgt tactcgcttt taacagggca acggaacacc cgcccagagc
     4261 ataaccaaac caggcagtaa gtgagagaac aatgtcaaac aaaccctttc attatcaggc
     4321 tccttttcca ctcaaaaaag atgatactga gtattacctg ctaaccagcg aacacgttag
     4381 cgtatctgaa tttgaagggc aggagatttt gaaagtcgca ctcgaagcgt taactctgtt
     4441 ggcgcgtcag gcgtttcatg atgcgtcgtt tatgctgcgt ccggctcacc aacaacaggt
     4501 ggccgacatt ctgcgtgacc cggaggccag cgaaaatgat aaatatgtgg cgctgcaatt
     4561 cctgcgtaac tccgacatcg cggcgaaagg cgttctgcca acctgtcagg ataccggcac
     4621 tgcgattatt gttggtaaaa aagggcagcg tgtatggacc ggtggctgtg atgaagcggc
     4681 gctggcgcgc ggtgtctata acacttatat cgaagataat ttgcgctact cgcaaaacgc
     4741 gccgctggat atgtataaag aggtgaatac cggcaccaat ctgccagcgc agatcgatct
     4801 ttatgccgtt gatggcgacg agtacaaatt cctctgtatc gccaaaggtg gtggttcggc
     4861 aaacaagacg tatctctatc aggaaaccaa agcgttacta acgccgggga aactgaaaaa
     4921 ttacctggtt gagaagatgc gcacgctggg tacggcggcc tgtcctccgt atcatattgc
     4981 gttcgtaatt ggtggaactt ctgcagaaac taaccttaaa acagtgaaac tggcttccgc
     5041 taaatactat gacgaactgc caacggaagg gaatgagcac ggtcaggcat tccgcgatgt
     5101 ggaactggaa aaagaattgc tgatcgaagc gcaaaatctc ggtctgggtg cgcagtttgg
     5161 tggtaaatac ttcgctcacg acatacgcgt gattcgcctg ccacgtcacg gcgcatcctg
     5221 tccggttggt atgggtgttt cctgctctgc tgaccgtaat atcaaagcga agatcaaccg
     5281 tcaggggatc tggatcgaaa aactggaaca taatccaggt aaatatatcc ctgaagagct
     5341 gcgtaaagcg ggagaaggcg aagcggtgcg cgttgacctt aaccgtccga tgaaagagat
     5401 cctcgcacag ttgtcgcagt atcccgtttc tacacgctta tcgcttaacg gcacgattat
     5461 cgtcggtcgt gatattgctc acgccaaact gaaagagcgg atggataacg gtgaagggct
     5521 gccgcagtac attaaagatc atccgattta ctacgcgggt ccggccaaaa cgccggaagg
     5581 ttatgcctcc ggttctcttg gcccaacgac cgccggacgg atggattctt atgtcgatca
     5641 actgcaagcg cagggcggaa gtatgatcat gctggcgaaa ggcaaccgca gccagcaggt
     5701 gacggatgcc tgtaaaaaac acggcggctt ctaccttggc agtatcggtg gtccggccgc
     5761 tgtattggcg cagggaagta ttaagagcct ggaatgtgtt gaatatccgg aactgggaat
     5821 ggaagccatc tggaaaattg aagtggaaga tttcccggcg tttatccttg tggatgataa
     5881 aggaaatgac ttcttccagc agatacaact cacacagtgc actcgctgtg tgaaataaac
     5941 agagccgccc ttcggggcgg tttttttaca tggcacgaaa gaccaaacat ttgttatcaa
     6001 atagtaaata ataagtgagc taaaagttgc ttaacgaaag caaaacagaa agaaaaaatt
     6061 aaacaggtga ggagcaggcc atgaatacag tacgcagcga aaaagattcg atgggggcga
     6121 ttgatgtccc ggcagataag ctgtggggcg cacaaactca acgctcgctg gagcatttcc
     6181 gcatttcgac ggagaaaatg cccacctcac tgattcatgc gctggcgcta accaagcgcg
     6241 cagcggcaaa agttaatgaa gatttaggct tgttgtctga agagaaagcg agcgccattc
     6301 ggcaggcggc ggatgaagta ctggcaggac agcatgacga cgaattcccg ctggctatct
     6361 ggcagaccgg ctccggcacg caaagtaaca tgaacatgaa cgaagtgctg gctaaccggg
     6421 ccagtgaatt actcggcggc gtgcgcggga tggaacgtaa agttcaccct aacgacgacg
     6481 tgaacaaaag ccaaagttcc aacgatgtct ttccgacggc gatgcacgtt gcggcgctgc
     6541 tggcgctgcg caagcaactc attccgcagc ttaaaaccct gacacagaca ctgagtgaaa
     6601 aatcgcgtgc atttgccgat atcgtcaaaa tcggtcgaac ccacttgcag gacgccacgc
     6661 cgctaacact agggcaggag atttccggct gggtagcgat gctcgagcat aatctcaaac
     6721 atatcgaata cagcctgcct cacgtagcgg aactggctct tggcggtaca gcggtgggta
     6781 ctggactaaa tacccatccg gagtatgcgc gtcgcgtagc agatgaactg gcagtcatta
     6841 cctgtgcacc gtttgttacc gcgccgaaca aatttgaagc gcttgcgacc tgtgatgccc
     6901 tggttcaggc gcacggcgcg ttgaaagggt tggctgcgtc actgatgaaa atcgccaatg
     6961 atgtccgctg gctggcctct ggctcgcgct gcggaattgg tgaaatctca atcccggaaa
     7021 atgagccggg cagctcaatc atgccgggga aagtgaaccc aacccagtgt gaagcattaa
     7081 ccatgctctg ctgtcaggta atggggaacg acgtggcgat caacatgggt ggcgcttccg
     7141 gtaactttga actgaacgtc ttccgtccga tggtgatcca taatttcctg caatcggtgc
     7201 gcttgctggc agatggcatg gaaagtttta acaaacactg cgcagtgggt attgaaccga
     7261 atcgtgagcg aatcaatcaa ttactcaatg aatcgctgat gctggtgact gcgcttaaca
     7321 cccacattgg ttatgacaaa gccgcggaga tcgccaaaaa agcgcataaa gaagggctga
     7381 ccttaaaagc tgcggccctt gcgctggggt atcttagcga agccgagttt gacagctggg
     7441 tacggccaga acagatggtc ggcagtatga aagccgggcg ttaatctgca acatacaggt
     7501 gcagccgtgg aatgatcaaa cgaagcggct gcgcctgtgg tttataacgg tgctgcacat
     7561 tgtcggcatc gtaatttaac aactcaccaa cgtctggcac gcccgcacca ttatcccgat
     7621 taatcagtgc aatcagcggt gtagggcagg cgtgttgcac ttgtttttga tctcctttgt
     7681 accagacgcg ggcaatcggc tgcaccttca ccggacgttt gatttttaac ttcgcgttct
     7741 gtggcagggc agcgatatcc tgatactctc gctccagttt tctttgccac tcctcgcgcg
     7801 tccacggtgc gacactgcgt ggtgatttca ggcttttttc cagctgtgcc aggacttcat
     7861 cacgatgcaa attcttaatg atatgtttat tagcccaacc aaagcgtaaa gtggcggggt
     7921 cgtgcagaac ggtgagcgtg cggtaagcat taagggtgat cagccccggc aaatgacgat
     7981 gcacccattc aaaacgtgcc gcggtgggga gttctgattc aaccgtgacg atatgctcga
     8041 acgtggtctt gagtttattg atgtgctgaa tatgactgac caacgctgct tgcgaaaggt
     8101 tatcgacctg gtaacacaac acgccaggca gacggacagc ggccttgctg ctgcgatttt
     8161 cggactgttg ttgaataaat aaatggcgga aatgacgcaa cgccagcgac tgcgcgtcgt
     8221 tgccgagatg ttgttttacc tcaatacgat taagcggatt atgctcatcc tcttttttta
     8281 cctccggcaa agagaacacg cgggcaacca atagcttgtg ttgctcaaga tgagcggcaa
     8341 atgtagccag ctcttgttcc atctggcgaa aggtagtgtt gagtcggtct acgagatcgt
     8401 aacgcgccat aatattaacc actttagtta caacatactt attttatagc acagtcgtgg
     8461 tgacttgacc atcgtgcgcg tagtgtcagg gagaggtaaa ttgcgggatg ttatgccata
     8521 acggccagct aaacgagaag cgagcaccac ccagttcgct ggtgtcacaa ttaaccgtac
     8581 cgcccattgc cagtgctata gagtggacaa ttgccagccc cagcccgcag ccgccggttg
     8641 agcgatcccg gctgggatcg aggcgaacaa aaggttcaaa gatatgttcg cggttttctg
     8701 gggcaatccc tgggccatca tcctcaacaa ttaatgtcgc tctattcccc gacagtagca
     8761 ggctggtttc aaccgttgaa tggcagtacc gcagggcgtt attgagcaaa ttatccagca
     8821 cgcgctccat taagcgcata tccaacgccg cataatggcc ttgcacgagc gttttaatcc
     8881 gtaccgtttt atcgggcgtt actgcctgaa tgtctgccag atgcgttgac agccacaacg
     8941 gcaggtctgg ttcgctaaga tgaagctcgt tttgtgggcg atcgagtcgg gcataagtca
     9001 gcagctcttc aattaaagct tcaagttgac tgatatcacg attcaacgcc tgggattcgg
     9061 cggcgctcag gttatcgctc atctccagtc gataacgcag gcgcactaac ggtgttcgca
     9121 gttcgtgagc gataccgtca ataagctgtt ttttgctggc aatcaaggcg ttgatattgt
     9181 ccgccatctg gttaaatgcg ataccaagtc gttcaaagct cgaaccctca tcaaagtgga
     9241 tacgctcatt gagatgccca tcgccaaatc gttgcgccgc tgcttccagt tttaacatat
     9301 cctgccagtg cggacgcatc cagataaaca ccggaaaggc gagggaaata gcaataaaag
     9361 cgatcagggc gatatccagc aatcgcatct gatggagata ataaagataa ggaacaggac
     9421 caactgccag cacgtagtgg ctgcgcggga tacgctgcaa aaacgtgtac tgatcgtcca
     9481 gggcgacaat ttcgccgcca cgcagtcggt gcatggaaat atcatcaaga tggtatttac
     9541 tcaatggctc gacacgcaga tcgaaagaga gatttaaatc catctctttc agcgtcttac
     9601 cccagtcgtg tggggggatc tcacgcaatt cgctgcgcat cagatacagc gaactgttca
     9661 tcaaatcatc cagcgactgt ttgcccgcgc gttcggcggt aaatttgtac accaacccaa
     9721 ccagcagaga catcacaagg aagcagacaa acaataacag gtaaaactgg ataaacagtt
     9781 ttttcatcgc ttattcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
     9841 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
     9901 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
     9961 cgcggtccat gatttgcccg gcatgggtag ctaattccca caataattcg aaatcagctg
    10021 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatggga tcgatggtca
    10081 acgtgccgaa atgcagggct ttgtagggag tcagagacgt ttcctgaaga cctttggtca
    10141 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    10201 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    10261 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    10321 aaatggtcat gccatccttg cctggtagca tgatgtcgag taacaccaaa tccggacttt
    10381 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    10441 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    10501 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    10561 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    10621 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    10681 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    10741 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    10801 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    10861 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    10921 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    10981 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcagctggt aagtaagtaa
    11041 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    11101 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    11161 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    11221 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    11281 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    11341 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    11401 agtggtgtac ctggtttttc cgccatccac gcactggcgt tatgcaaaat agcacgcaga
    11461 ccatgggtgc tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    11521 tcagcctgaa tacactgcgc acctgcctta atcagtccat caatggctgg atagtgcgtc
    11581 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccaggcgag ggcgaggccg
    11641 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggctttacc catcgttatc
    11701 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    11761 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    11821 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gcgcatacag aacaacggat
    11881 aacagcaagt gcattggacc agaggcatac aataaccata agccataaat gcaggcaccg
    11941 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    12001 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    12061 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagcgaggcg
    12121 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagttgca
    12181 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    12241 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    12301 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac gcccagcgaa
    12361 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    12421 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    12481 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccacacggg tacgccaagt
    12541 gcaagtccgg tgaagtcgag cttgaaagta tccagtttga acatcatcat cgccagcaca
    12601 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    12661 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    12721 atcgattgcc aggtattacc atcgccgaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    12781 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgactgc gcacagccag
    12841 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    12901 ataccgccgt caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    12961 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    13021 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    13081 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    13141 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    13201 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    13261 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    13321 cggtcaggtt attaccacgt tcctgccact ggcgggtgat gtggtaatac ttagcacctt
    13381 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    13441 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    13501 ctttagtgcc gtcaggaagc gtgacagtgt aacgtccgcc ttttgatgac tgggtttcaa
    13561 agaaacgacc gacttcagaa cttggtgatg cggtagtcgc aacacctggg atctcaactt
    13621 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    13681 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    13741 tacgctggtt gccgccctgg ttggcatcga tttgacgaac aatgtagaaa gagtaggcac
    13801 ctttcgcttt tgctgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    13861 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    13921 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    13981 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    14041 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    14101 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    14161 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    14221 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    14281 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    14341 atttttcata tcacattcct taagccaatt ttaatcctgc tcaaatgacc gtctatgctt
    14401 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    14461 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    14521 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    14581 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    14641 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    14701 gagcggcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    14761 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    14821 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    14881 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    14941 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    15001 aaattgcgtt actgaatcct gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    15061 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gattctgtgc
    15121 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaac atcgccggtt
    15181 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaattactg
    15241 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgcgggtgtt gcaggtctgg
    15301 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    15361 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    15421 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    15481 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    15541 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    15601 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacacc gtgccgggtg
    15661 aaatcttcac tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    15721 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    15781 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    15841 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    15901 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    15961 cgtggcgtaa atacgcattg atggcgctgg caatcatcct tttcggttgg ctggcaagcg
    16021 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    16081 attacgtggt gtggaatgta tcgcatgcgc tgcatacacc gttgatgtcg gtcaccaacg
    16141 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccagggc ggctgggtta
    16201 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    16261 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    16321 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    16381 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    16441 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    16501 gtcattggtg gggcaattgg tatccgtctg gcgaagaaag ttgaaatgac cgaaatgcca
    16561 gaactggtgg cgatcctgca tagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    16621 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    16681 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gttcggtggt ggcgttcggc
    16741 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    16801 ctggcggctc tggtcgtttc cttcctgctg ctgattgtat ttgttcgcac ggatagcgtc
    16861 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    16921 ttagtcgcct ccatcggtgg tgcagatatg ccagtggtgg tgtcgatgct gaactcgtac
    16981 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    17041 ggtgcgctgg tcggttcttc gggcgctatc ctttcttaca ttatgtgtaa ggcgatgaac
    17101 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    17161 gatcaggaag tgggcgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    17221 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    17281 cctgtcgctg aaattaccga gaaactgcgc gctcgtggta tcaacgtgcg tttcggtatc
    17341 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    17401 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    17461 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    17521 cctattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    17581 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    17641 caaatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    17701 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    17761 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgttaacg gcacggaaag
    17821 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    17881 tgtggaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    17941 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    18001 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    18061 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    18121 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    18181 actgtcaatt gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    18241 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    18301 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    18361 cgcgttcgga tcaatgtaat gcgccagctg gtcaactgag acgtcaatcc ctacgcgttg
    18421 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    18481 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    18541 gatgatggtc atcaaaatcg acaccgccag tacacgcggc actcgccagc ggaccatgtg
    18601 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    18661 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    18721 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccattt tttcttcctt
    18781 tgctaataat aatgtctttt tcgctccgtc atcataacga aaatcaaacc gctcaggtaa
    18841 agaagtgaaa atattttgag ttaattctta agctatgata caaatcaggc gtgttcaact
    18901 accgaggaca attatcatcc gcgatgacga gaagcaacac tgcggataat tgtaatatta
    18961 tggacaattt gttcaggacg ttattttcaa catttaccca tcttcgtacc tcatccacta
    19021 tcttgctcgt aggtgagcag cactggcgca acgcgcttta gtcctttctt ctgcctaagc
    19081 ttgccctatg cgcggggctt gaccacttta ctttggtttc gctgaattaa gcgaaaatta
    19141 aaataattct cttgcaggag aaggacaatg tatatttatt ggattttatt aggcctggct
    19201 attgctacag aaattaccgg tacgctgtca atgaaatggg cgagcgtcag tgagggaaat
    19261 ggcggcttta ttttaatgct ggtgatgatt tctctgtcgt atatatttct ctctttcgcc
    19321 gttaaaaaga tcgccttagg cgtagcttat gcgctgtggg aaggtatcgg tattttattt
    19381 attaccttgt ttagcgtttt gttattcgac gaaagtttat cgctgatgaa aattgccggg
    19441 ttaaccaccc tggtcgccgg gattgtgttg atcaaatcag gtacccgtaa agcgcgtaaa
    19501 cctgaactgg aggtgaacca tggcgcagtt tgaatgggtt cacgccgcct ggctggcatt
    19561 ggcaatcgtg ctggaaatcg ttgctaacgt ctttttgaaa ttttctgacg gctttcgtcg
    19621 caaaatattt ggcttgctct ccctggcggc ggtgctggct gcctttagtg cgctttctca
    19681 ggccgtaaaa gggatcgact tgtctgtcgc ttatgcattg tggggcgggt ttggtattgc
    19741 cgccacgtta gccgcaggtt ggatcttgtt tggtcaacgg ttaaatcgta aaggctgaat
    19801 tggcctggtc ttgctgttgg ctggaatgat catggtgaaa cttgcctgat gaacacgctg
    19861 cccgcactgt cgggcagcgt ttgaacatta tttttgcgac agttgatcca gcttgtcgcg
    19921 aaaaccggta acagaaatgg cccggttatc ggcgcgccag cgatctttcg cggcaggagc
    19981 cgaactttgc accccaatta attgccagcc gtcatcggta tgcaacatca gaggcgaacc
    20041 gctgtcaccc ggcaaggtat cgcactgatg tgacatcacc gacgtttgcg cccagccagt
    20101 cacttcacag ttttgatgac tgtataacgt atcgagatga tcttcaggat atcccgcctg
    20161 agtcacttta cgaccagccg attttaatgc ggcagtaagc gcggctttat ctccctcaaa
    20221 taacggcaac ggtgtaatgc cagaaggggg attacgcagc acaatcaagc cgaagtccca
    20281 tggtgcagct gcgggaggaa caatccaacc atccccatct gcttttaacc gctttcccag
    20341 tgtcggatca acgcggcctt ctatgtcgtg gatgtcatag cgccaaagac ctttatttga
    20401 cacaaaacgc agcgctaccg ccttatccgc tttaccctta ggcggcgtca ataaacagtg
    20461 tcctgccgtt aatgccagat tgggtgcaat cagcgtcgct gtacataaat tgccgctggc
    20521 cgtttccagt tgcccaaccg catcccacgg tgattgggtc gtgtcattca ctggcacacg
    20581 atcatcatga ccaaaaaaca gggtgctgac ctcatcgttt gccgatttgg caacgtctgg
    20641 tttatctgca aacacaaaag cagacgtcaa actaattgca cccaacacta cagcaatggt
    20701 tgtacgcata tcacactctg gtgggtaatt atgattatta aaagcaaacc ctcataaata
    20761 ctatagacgg gacgggatga aagtgggagt aaaatcagat aactacagtc aggaaagata
    20821 aaaacgggcg gcaatcagtg cgcataaaat aaggatgata agaatgaact caaagcgata
    20881 acggcccacc atactaccct ccgctaaagg cggtgctaaa cgttacccgc ttagcaccgt
    20941 aattttcagg cgcgaggggg cgcgccggca ttactgttga aaacttacgc tgcgggttgt
    21001 gcagcaggtt ttgccggttg ctgatggctg tgtttcccgg cgtgtttctt cgctgcctgc
    21061 gctttttgtt caggggcttt ctgttcagct ttcgtatttt tatgatgctt tttagccgcc
    21121 tgcgctttct gggcaggtgc tgctttatgc tgttttttat gatgtgtagt tttcgccggc
    21181 gctgctttgg tggtcgtcgc agtcggagca ggtgtggtcg cagtctctgc agcaaaggcg
    21241 gcagaagaca gacccatagc agcggcaaca accagaccta atactttttt cattgtcata
    21301 ccctcaattt gttttttcat ttaaccccac tgcggggccg ttgaaataac tatatccctg
    21361 ggaatggcag acttccgtga gtggttggtt tcagcgtgta acgatatgta caaacgctga
    21421 ataaaataca gcattgataa taacgtattg tgacttaagg gaaatttagc taccaataat
    21481 agtagtcttg atcgggttaa ctattttact acttacaaag cggtagaata actgcgcatc
    21541 agtaataaat gacacacagc aaaatgaatc cgtttatttg ggtacttata atcctgatga
    21601 cgctagacgc gctgcgggaa ttggctggcg cttcgtctat tttaggatgg ctattaacgt
    21661 tggtttgagc aagcaataag tccggacgtg tagttaccgc agtccgggct tatttttcag
    21721 gcgtgcagac gacgatgcaa acgcgtcccg accagcagag caatgaccag catcagagca
    21781 ataaatgcgc cgacgccgtt ccagccatag ttatgccaga aaacaccacc cagcgtcccg
    21841 gcaatactcg accccagata gtaactgaac agatacagcg aggaggcctg gcctttagcg
    21901 cgttttgcgc gggggccaat ccagctgctg gcaactgagt gggctgcgaa gaatcctgct
    21961 gagaagagta acattccggc aaagatcagc cacagcgagc tgaataaggt catcagtaaa
    22021 ccaaacagca taacccccgt cgaaaacaac atcaccggac cgcgcccgta gcgggtggtc
    22081 atggttccgg ctttaggtga gctccatgta ccggtcaaat aagccagcga taataagcca
    22141 accacggcct gactgacatg ccagggggaa agcatcaacc gatagccgat gtaattaaac
    22201 agcgtgacga acgaccccat cagcaaaaag ccttctgcga acaataacgg taatccccgg
    22261 tcacgccagt gcagacgaaa gttgataaac aacgtcttag gtcgcagcga agtcgggcga
    22321 aaatggcgtg attcagggag gattttccag aacatcaacg ccgaggctag cgcgaaacaa
    22381 ccgattgccg ccagagcaat tcgccagttg aaaaagtccg tgaagacacc actaattaag
    22441 cgcccgctca tgccgccaat tgagttgccg ctgatataca accccatcga aaaggccacg
    22501 aaactggggt ggatttcctc gctaagataa gtcatgccaa cagctgccac gccacttaac
    22561 gaaagcccaa tcaaggcgcg cataatcaaa atgccgtgcc agctggtcat cattgtcgaa
    22621 agtaacgtac aaatggaggc caacagtagc gccgtgacca tcactggttt gcgaccaatg
    22681 gcatcggata gggggccagt aaacagcaaa ccaatagcca acatcgccgt ggaaatggac
    22741 agtgaaatac tactgttcgc gggcgttaag ccaaactcct gcgaaagcac cggaaggata
    22801 ggctgcacac aatagagaag tgcaaatgtt gccagtccgg cagagaacag cgccagggtg
    22861 acgcgcataa attgcggcgt accgcgttta ataaattgat ttggctgaga aatgctttgc
    22921 ttgtcagtgt cgcttgccgg agcgccatca acagttgtag tacggctcac ttgaaatcct
    22981 tgctaaatat gcctgtggat caggcttata catagggtag gaaaatcgaa ttgttctgtc
    23041 taatatatta ataatctcaa ataagatgtt ttaaatatga atattgaact tcgtcatctg
    23101 cgttactttg ttgctgttgc ggaagagctg cattttgggc gcgcggctgc ccgcctgaat
    23161 atttcgcaac cgccgctaag tcagcagatt caggcactgg agcaacaaat cggtgcccga
    23221 ctgctggcac gaactaatcg cagtgtattg ctgacggcag caggaaaaca gtttcttgca
    23281 gatagtcggc aaatcctgtc tatggtggat gacgctgccg ctcgcgctga aagactgcat
    23341 cagggtgaag cgggggagtt gcgtattggt tttacttcgt ccgctccttt tattcgggcg
    23401 gtgtctgata cgttatcgct gtttcgccgt gattatcccg atgtccattt acaaacccgc
    23461 gaaatgaaca ctcgtgagca aatcgctccg ctcattgaag gaacgctgga tatgggattg
    23521 ctgcgtaaca cagcgttacc ggagacgctt gaacacgcag tcatcgtcca tgaaccgctt
    23581 atggcgatga tcccgcacga tcatcccctg gcaaataacc cgaatgtaac gctggctgaa
    23641 ctggcgaaag aacccttcgt cttttttgat ccgcacgtcg ggacagggct gtatgacgat
    23701 attctcgggc tgatgcgacg ttaccatttg acgcccgtca tcactcagga ggtgggcgag
    23761 gcaatgacca tcatcggtct ggtttccgcc ggtctgggtg tttcaatttt gcctgcgtca
    23821 tttaaacgtg ttcagctcaa cgaaatgcgc tgggtgccga ttgcagaaga ggatgcggtt
    23881 tctgaaatgt ggttggtctg gccgaaacat catgaacaaa gtccagctgc gcgtaacttt
    23941 cgtattcatc tgctgaatgc tctcaggtga gggaaatttc agcgaaaaag cccgaaaaat
    24001 gtgctgttaa tcacatgcct aagtaaaaat ttgacgacac gtattgaagt gcttcaccat
    24061 agcctacaga ttatttcgga gcgcgaaaat atagggagta tgcggtggtt gctgaaaacc
    24121 agcctgggca cattgatcaa ataaagcaga ccaacgcggg cgcggtttat cgcctgattg
    24181 atcagcttgg tccagtctcg cgtatcgatc tttcccgtct ggcgcaactg gctcctgcca
    24241 gtatcactaa aattgtccgt gagatgctcg aagcacacct ggtgcaagag ctggaaatca
    24301 aagaagcggg gaaccgtggc cgtccggcgg tggggctggt ggttgaaact gaagcctggc
    24361 actatctttc tctgcgcatt agtcgcgggg agattttcct tgctctgcgc gatctgagca
    24421 gcaaactggt ggtggaagag tcgcaggaac tggcgttaaa agatgactcg ccattgctgg
    24481 atcgtatcat ttcccatatc gatcagtttt ttatccgcca ccagaaaaaa cttgagcgtc
    24541 taacttcgat tgccataacc ctgccgggaa ttattgatac ggaaaatggc attgtacatc
    24601 gcatgccgtt ctacgaggat gtaaaagaga tgccgctcgg cgaggctctg gagcagcata
    24661 ccggcgttcc ggtttatatt cagcatgata tcagcgcatg gacgatggca gaggccttgt
    24721 ttggtgcctc acgcggggcg cgcgatgtga ttcaggtggt tatcgatcac aacgtggggg
    24781 cgggcgtcat taccgatggt catctgctac acgcaggcag cagtagcctc gtggaaatag
    24841 gccacacaca ggtcgacccg tatgggaaac gttgttattg cgggaatcac ggctgcctcg
    24901 aaaccatcgc cagtgtggac agtatacttg agctggcaca gctgcgtctc aatcaatcca
    24961 tgagctcgat gttacatggg caaccgttaa ccgtggactc attgtgtcag gcggcattgc
    25021 gcggcgatct gctggcaaaa gacatcatta ccggggtggg cgcgcatgtg gggcgcattc
    25081 ttgccatcat ggtgaattta tttaacccac aaaaaatact gattggctca ccgttaagta
    25141 aagcggcaga tatcctcttc ccggtcatct cagacagcat ccgtcagcag gcccttcctg
    25201 cgtatagtca gcacattagc gttgagagta ctcagttttc taaccagggc acgatggcag
    25261 gcgctgcgct ggtaaaagac gcgatgtata acggttcttt gttgattcgt ctgttgcagg
    25321 gttaacattt tttaactgtt ctaccaaaat ttgcgctatc tcaatttggg ccaggaaagc
    25381 ataacttaga ctttcaaggt taattatttt cctggtttat atttgtgaag cataacggtg
    25441 gagttagtga tgctgaagcg tttctttatt accggtacag acacttctgt agggaaaacg
    25501 gtggtttccc gcgcattgct acaagcgtta gcctcccagg gaaaaacggt tgcgggatat
    25561 aaacccgtag cgaaggggag caaagagaca cccgaagggc tgcgtaataa agatgccctg
    25621 gtgttgcaga gtgtttcaac catcgaactg ccttatgaag cagttaatcc tatcgcgtta
    25681 agcgaagaag aaagtagcgt ggcgcacagt tgcccaatca attacaccct catttcaaac
    25741 ggcctggcaa acctgaccga aaaagtcgat catgtcgtgg tagaagggac tggcggctgg
    25801 cgcagtctga tgaatgattt gcgtccactc tctgaatggg tggtgcagga acaactgccg
    25861 gtgttgatgg ttgtcggtat tcaggaaggt tgcattaacc atgcactgct aacagctcag
    25921 gcgattgcca acgacgggct gccgctcatt ggctgggtgg ctaaccgaat caacccagga
    25981 ctggcgcatt atgcggaaat cattgatgtg ctcggtaaaa aacttccggc accgctcatt
    26041 ggtgaactgc cttatctgcc gcgcgccgaa cagcgtgaac tggggcaata catccgctta
    26101 gctatgctgc gcagtgtgct ggcggtagat agagtcacgg tgtaacgtcc gcgagattac
    26161 cgacgcaatt acgcaggcaa tcaataaacc ggggagtagc tgatactccc cggtcatttc
    26221 acatatcatc aacgtcgaca taatcggcgc gtgcgtggtt gccgccaaca gtgtcgccat
    26281 cccggtcaat ccgagtaaaa gcgtaatttc ttcgccatca gggaaccata atcccaggct
    26341 acgaccatac aacatgccaa tggcaagacc gataaatagc gtcggtgtaa agacaccacc
    26401 gggtgcgccg gaaccgctac tcgccagcac ggcacacagt ttacaaagga agatcccggc
    26461 aatgatcatt aacagtggcg gggcggttaa aaaggattgt acggtgctat agccgttgcc
    26521 ccacactgca ggtgtgaaca gggaaagcag acccacgatc aacccgccta gtgccagttg
    26581 ccagggcggc gcaagtttga gacccacaaa tccacgatga caggcgttca ttaacgttaa
    26641 caacagtggt ccgcacagac ctgccagcac acctgtactg ataatcagcg catagtcacg
    26701 agcctgaacc gtcactgaga gttgtacgtt gtagagtaac gcgtcgctat gattaatcag
    26761 attgctaacc agcaatgcca cgacggcgga aataatcacc gggccgagag aggccaacat
    26821 catagtgcca aacagcactt cggctataaa taaactgcca gcaagcgggg cacgataggc
    26881 cgcagccatt cccgccgcgg ccccacaggc gatccataat ttccactcct ggcgtggcgt
    26941 aaaacgttgg gcaaaacagg aggcggcaag ggcagctaaa agaatcatcg caccttcgcg
    27001 accaattgca ctgccgctgg ttactaccag cagagaggca agcgatttaa ccaggcttgc
    27061 tgcgtaatcg aactgtccat cggtttgcaa cgcttccatg taatcggtcg gcgcatgagg
    27121 gcgttgttgg gtaaatttct gccagcccat cagcaacaaa cccgccgcca gtccgccgag
    27181 cgccggagtt agcaaccgtc gccaggggga aaggtttgtc gctgcattga ccagactgcc
    27241 ggagtcatta ttgaggaaca accactccag tagtagcatc gcatgacgaa acccggcaac
    27301 ggcaaaggcc gcgagaatac cgacgactgt tgcgataagc agacggcgga acatagtgcg
    27361 cagatccgga tagatgtgta gatgatgcat gagcagaaat gggcaatgaa acacgggcga
    27421 ctattttgcg gcaaaagagg ccagccgcaa aagcagtgcg tgctttaaga gataaaaaaa
    27481 cgtgacactg taacctatta ttgccggatt gcgcgtaatc gtcaccatcc ggcaatatta
    27541 cggtgatcct atcgaaacag cggtttaacc gcgacaggaa ttaacagttg tgattgccat
    27601 tgcgccagcg ttaagcgagc cagttcaccc agtgcacggt agaaagggtg ttctgctttt
    27661 tcgataaaaa catcaagaaa acgtgttgac cacggaaaaa ggtgccatgc cagcaattct
    27721 tcgcattctg tctggcgacc attctccgcc aaccacgcag ccatcaacag cagtgaacca
    27781 aaatgatctt ccggttcgtt ttgcttcatt tcaaactgaa tgcctttctc gcgcatccac
    27841 tgacgaagtg ccaatgttga atcgccaaac agcacagatt cgcgatccag ccagaccgac
    27901 ccccacggcg gagacggcag tgcccacggg ccgacaaaca aacgctgcca ggcctgggcg
    27961 tgagtctctt cacactgtgt ctgaaaagcc gtcaccagcg gcgctaatga cgcttctggt
    28021 agaggccact gagtttccca gccgtcactg gtgagtaccg caaccagagg tgcggcttcc
    28081 gcactctctg gagcgtaata aaacagcgcg cccaacacgc gcgccgcgac agaaaaatta
    28141 tcttgctgtg aaaaatgggt cattcctaac atcctgaaat gtgcgggttg ccccgcacca
    28201 atttgttaac ctgcaatagc cattcctact gtcatgtgca ggccataaaa gagcacacgc
    28261 ccgatcatct caccgccaag tatgaggatt agccccagaa taagtccggc aacgtgaggt
    28321 tcacggctac gaatgagcgg gcaaagccag catccaagac ctgcgcataa caataccaca
    28381 cgccagacct gtaaggaggc gtaatctggt accagtgcgc tggcttgttg cacggatgag
    28441 tgaatagacg ccagagacaa accttgcagc acaatcaccc cggcacaagc gatcagcgcc
    28501 agaacgctaa tgatggcaaa tggtgtggta ttaaatgtaa cgcgggctgc ccgcagaatt
    28561 gcggcagcaa gtattggacc gctcaacaat accgtcagga agaaagccag cgtagtgtaa
    28621 ccgttatgcc aggttggcac agtgtcgatt tgatacacgc aggtcatcat ccagacgaaa
    28681 atgacgccga gcgccatgct gacaagtaac cagagtttcc ccaacgcttg cggcatttta
    28741 ccgatgaccg ccaccagcca ccatagtccg ccaacggcaa aaaatatgga accggcagca
    28801 atctcgttac tcagaccaga agctccgatt cgattcagcg agttaaacgc ccgcagtggc
    28861 gatcccaggt gcatgacaga ggcaataaac ccgatgccca tcaacagcca gaggaaaaac
    28921 atgccacgga caatacgctg acggtctgcg tcatttttgg cggcgaacca gccaattccg
    28981 ctaacaatca gcgcgcctac tacgcattga cccaatacgg taaagataac cagtggccac
    29041 tcatgccatc catttcccat tttacacttc ctccggatta gccagataac cagtggtatc
    29101 gccggtcggg cggctgttgg cgttaggttt gataacgata ttgggttttg tgaaatgcgc
    29161 gcgcggcagt ggcgcgacgg cagccagagt gccgtgcttc tggcgcagct cttcaattgg
    29221 accgaactca agtgcacgca gcgggcagga ttcgacacat atgggttgtt tcccctcggc
    29281 gacgcgcgaa taacaaccat cgcacttcgt catgtgccct ttttcagcat tgtactgtgg
    29341 cgcgccgtac ggacaagcca tgtggcagta gcggcagccg atacaaacat cttcatcaac
    29401 caccacaaag ccatcttcac gcttatgcat tgcgccgctg gggcagactt ttgtacatgc
    29461 ggggtcatcg caatggttac aggagatgga gagataataa gcgaacacgt tctggtgcca
    29521 gacgccatta tcttcctgcc agtcgccgcc agcgtattca taaatacggc ggaaactgac
    29581 ttccgggcca agatctttaa aatctttgca cgccagttcg caggttttac agccagtaca
    29641 gcggctggaa tcgataaaaa atccatattg tgtggtcatg ggctactcct taaacctttt
    29701 cgatctggac aagattgctg tgcgacgggt ttccctttgc cagcggtgaa gggcggtgag
    29761 aggtcagaat attgatactg ccgccgtgat cgacccggtc accaaacata tccgctttaa
    29821 gccacgcacc ttgcccgatg gcggtaacgc caggcagaat acgcggagtc acttttgcgg
    29881 caatcagcat ttctccatta ttgttaaata cccgcacggt atcgccatga cggataccgc
    29941 gtgcctgagc atcaatgggg ttgatccaca cctcttgtgg gcaggcctgc tgtaacacat
    30001 caatattgcc gtagctggag tgggtacgcg ctttgtagtg gaagcccgtt aactgcagtg
    30061 gataggtttt ccgcagggga tcgtcccagc catcaaaacc tggggtatac gcaggaaggg
    30121 gatgaataat ttcatctttt ttcaattccc aggtatctgc aatcttcgcc agtcgttcag
    30181 aataaatttc gattttcccc gaaggtgttt tcaacgggtt tgcctgtgga tcttcacgga
    30241 atgcgcggaa agcgacgtag tgttcttccg ggcatttttt cttaaagatc ccggtcgttt
    30301 tcatctcctc gtagtcgggc atctcagggt tacgttcctt cgttttcgca tggagatatt
    30361 tgatccattc atgctgactg cgaccttcag taaaggtttg ataaacgtct ggtcctaagc
    30421 gtttggcgac ttcactcagc atccagtaga tgggtttgcg ttcaaatttt gctgaggttg
    30481 cgggttgggc gaggatcaca tagcccatat tccctgcaga ttcatgagag ataaggtctt
    30541 cttgctctgt tggcatcagg tcgggcaaca ggatatcgca atacttagcc gaggccgtca
    30601 tgaagtggtc aatgccaaca atcatctcgc acttgctgtc atcctgaagc acctcatggg
    30661 tgtgattgat gtcgccatgt tgattgatca atgtgttact ggcgtagcac cataaaaact
    30721 tgatggggac atccagtttt tcttttccac gaacaccatc acgggtcgcg gtcatttccg
    30781 taccatggtc gatggcatct gtccatgtaa agacggaaat ctgcgtttta acaggattct
    30841 caagcatcgg gaaccattct acccccagat cccagctacc ttcgcgtaca cctgagttgc
    30901 cgccgtttat gccgacgtta ccggtgagaa cggaaagcat ggcaatagcg cgggacgttt
    30961 gctcgccgtt ggaatgtcgt tgtggccccc aaccctgaca aatataagca ggttttgctg
    31021 aaccgatctc tcgtgccaac tggataattt tttctgccgg gatgctggtg atttttgctg
    31081 cccattccgg cgttttagct atgccgtcag gcccttcgcc cagaatatag gctttataat
    31141 gcgcgttacg tggtgcgttg gcgggcagcg ttttttcatc gtaaccaaca caatatttgt
    31201 cgagaaatgg ctgatcgacc atgttttcag taatcagtac ccaggcaatc gcacaggcca
    31261 gtgcgccatc ggtgccaggg cgaatgggca gccattcatc ttcacgcccg gcagcagtgt
    31321 cgttataacg tggatcgatg acgatcatgc gtgcgtttga acgttcgcgg gcttgctcga
    31381 cgtagtaagt gacaccaccg ccgctcatcc gcgtttctgc cgggttattt ccgaacataa
    31441 cgaccagttt cgtattggcg atatcatccg ggctgttgcc atcattggca ccgaacatat
    31501 aactcattgc ggcactgatc tgtgcggtac tgtagctgcc atagcgactg agaaaaccac
    31561 cgcaagagtt catcagacgg tacgggacgt ttgagttggt gatgtttccg ccatctacgc
    31621 ctgttccgta caggacatgt acagcctcat tgccgtaatc tttcaggatc cgccgaagat
    31681 tatcactgat ggtatccagg gcttcgtccc aacttatccg ttcaaattta ccttcaccgc
    31741 gcttgccgac gcgcttcatg ggatatttca acctatcagg atgattcatc cgtcggcgga
    31801 tagagcgccc gcgtaaacac gctcgaacct gatggttacc gtagacgtcg tcacctgtcg
    31861 tatcagactc cacccagtac acggtgtcat ctttcacatg caaacgtaac agacagcggc
    31921 tcccgcagtt aacggtgcag gaactccaga ccgctttctc ttctaccgga gcctctgccg
    31981 cccggaccat ttgggaaaat ggcagagtga aagcactgct tgccagcgca agactgccaa
    32041 gtgcggaggt tttcatcaga cttctacggc tgatttcagc cttcatgagc gcctctgtgg
    32101 tatggatttt catcattact cacttattgc ttttcaaaca aaatgtcatg ccagaattta
    32161 tgattgtcgt gggttatatt ttttcgatct cgaccagatt agtgtgctgc gggtttccct
    32221 tcgccagtgg tgaagggcgc agagtggtta gcgtattcac acagccgcca tggtcgattt
    32281 tatcgccaga catattggcc tcgtgccagg ctccctggcc catagcgcta actccaggga
    32341 gaatacgtgg tgttactttg gctggtagcc gaacttcgcc acgatggtta aacacccgca
    32401 ccatatcgcc gttggcaatc ccacgtttct gcgcatctat agggttgatc cacacctcct
    32461 gacggcaggc agccttcagg agatcaatat tgccgtaggt cgagtgagta cgggatttgt
    32521 aatggaaacc aaacagttgc agtgggaagg ttctacgttc aggggagtcc cagccttcaa
    32581 aggtagaggc ataaactggc agtgggctta tcacttcatc tttttccagt tcccaggtac
    32641 gggcaatttc cgccagcttg ctggaataaa tttcaatctt accggaaggt gttttaagtg
    32701 gatttgcctc ggggtcgtca cgaaatgctt tgtaggcgac aaaatgacca ttgggatctt
    32761 tacgcttata gatacccatt ttttcagttc gtcgtaagac ggtaacgccg gatctttggc
    32821 aagcattttg gcgtacagat gttgtaacca ttgttcctgc gtgcgacctt cagtgaactt
    32881 ttgatagacg tcaggtccaa gacgtttcgc gacttcactc aggatccagt aaatcggttt
    32941 gcgttcgaat ttttcgctgg tgacaggctg gaggaaaatg agatatccca tgttaccggc
    33001 gtagtcgtta ggaataatat cttcctgctc aacggtcatc aggtctggca gcagaatgtc
    33061 ggcatatttt gccgatgagg tcataaagtt ttcgatgacc acaatcattt cgcatttcga
    33121 ttcgtcctgc agaatttcat gcgttttgtt gatgtcagaa tgctgattaa cgagggtatt
    33181 tcccgcgtag ttccagatga acttaatggg cacatccagt ttatctttgc cgcggacgcc
    33241 gtcgcggatt gccgtcattt gcggaccatg atcgatagca tctgtccagc tgaagcagga
    33301 gattgacatt ttgaccggat tatccagcac cggcaggcgt tctatggtaa tggtataggt
    33361 cgattcacgc gcgccactat ttccgccgct gatgccgaca ttgcccgtca aaataggtaa
    33421 catagcaata gcgcgtgcag tcagttcgcc gtttgcctgg cgttgcggcc cccagccctg
    33481 gcagatataa gcgggttttg ctgtgccaat ttcacgcgcc agtttgatga tacggtctac
    33541 cgggataccg gtaatttgcg aagcccactg cggcgttttc gctgtgttat catcaccttc
    33601 accaagaata taggctttat agtgactatt tttgggtgca tctgcgggta aggttttttc
    33661 gtcatagccg acgcagtatt tatcgagaaa aggttgatca acgagatttt cgttaatcaa
    33721 tacccaggca atacccgcaa ccagcgcggc atcggtgccc gggcgaatag ggagccattc
    33781 atcttcacga ccagcagccg tatcggtata tcgcggatcg ataacaatca ttttggcgtt
    33841 cgatttctcg cgcgcttttt caagaagata agtgatgcca ccgccgctca tgcgggtttc
    33901 tgccgggttg ttaccaaaca tcacgaccag cttgctgttt tcaatatccg tggtgctgtt
    33961 gccatcatta ctgccgtagg tgtagggcat ggcacaggaa atttgcgcgg tgctgtagga
    34021 gccatactga ttgagtgaac cgccgtagca gttcatcagg cgtttgaccg ccgaggctga
    34081 tggcgaagag cgggtcatat tgccgccaac gatccccgaa gagtactgaa tatatacagc
    34141 ctcattgcca tattgttcga cggttttctt caggctactg gcgatagtat ccagggcttc
    34201 atcccagcta atccgttcga atttgccttc gccgcgtttg cccacgcgtt tcattgggta
    34261 attcaagcga tcgggatgat taatacgccg acggatggat cgaccacgca aacaggcgcg
    34321 tacttgatga ttgccatact cgtcgctgcc ggtgttgtca gtttccaccc aggtgacttc
    34381 attatcttta acatgtagac gaagtgcaca gcggctacca cagttgacgg aacaggcacc
    34441 ccagatcact ttttcgctgg cctgttgtac cgctgccgct gcactgcgca gggtgaacgg
    34501 taaagaaaaa ccgcctgcag ccagcgccag agaacctatc gcggttgatt taacgagtgt
    34561 tctgcggctg atgcccacca ttcggtcatt tttggacata actcactccc tgttctttat
    34621 cgttatataa atgtttatat attgaatatt tagcgcgcta acaatagagg gagtctaccc
    34681 attttgggtt aagaattatt aatccatatc aatagaaggg tatgagtaat aaggtgggat
    34741 tatgttgtat gttcaaatcg ccggatgtgt cgtatccggc gttcagtcga taatgtatta
    34801 ctgcggttcg gcaggcgcgc catcctgggt agactgcgcg ggagcagaga cgttaccgct
    34861 ggtggtgcgg gtatagagaa ttttatgcgt atcattagcg caatggccga cgacctggga
    34921 atcaggctga tcaacctggt cattgggtac aatacttaac gtgaagctgc tttcgggtac
    34981 gccattattg ataatgcgct gtgatatatc gctctgtatg cgctcacagg atcccggcgc
    35041 ggcgagtacc gcgggtgagg cgagggcgag cagaagcgcg gcacagcagg ttgagagttt
    35101 catcataagc tccttacgcg aagataactt ctttaagcat agcatttaac gtgtaaagta
    35161 ctgtatttgc tactatgatt gagaatcatc tctactctct ggtgactgtt gtgaaataca
    35221 aattactacc atgcttactc gcgatattcc tcacaggatg tgaccgcaca gaggtaacac
    35281 tttcatttac ccctgagatg gccagtttct ctaatgaatt cgattttgat ccgctgcgtg
    35341 gtccggtaaa agatttcact cagacattaa tggatgagca aggtgaagtg acgaaacgtg
    35401 tttctgggac tttgtcggaa gaaggctgtt ttgattcact cgaattactg gatctggaaa
    35461 ataataccgt ggtcgctctg gtactggacg ccaattatta ccgtgatgcc gagacgctgg
    35521 agaagagagt acgtttacag ggaaaatgcc agctagcaga attaccttct gccggggtga
    35581 gttgggaaac cgatgataat ggcttcgtga ttaaagccag cagcaaacaa atgcagatgg
    35641 aatatcgcta tgatgatcag ggttatccac tgggtaaaac cacgaaaagt aacgacaaaa
    35701 cattatctgt cagcgccacg ccatcaacgg acccgatcaa aaaattagat tacacagcgg
    35761 ttactttact gaataatcaa cgagttggta atgtaaaaca gagctgtgaa tatgacagtc
    35821 acgccaatcc ggtggactgt cagctgatca ttgttgatga aggagtaaaa cccgccgtcg
    35881 aaagggttta caccatcaaa aatacgatcg attattatta atgctattgt gcggtcggct
    35941 tcaggagagt ctgacccggt gttttgtgct ctgccagata ctgatgctgg aatatacaca
    36001 tgcgaatggc attacgatat tgaccattaa taaagaactc gtgcatcaat tcaccttcaa
    36061 ccgtaaagcc aagcttgcgg taaatgtgaa tcgctttttc attctcttta tcgacgatca
    36121 gatacagctt atagagattg agaacggtga agccatagtc cattgctaat ttggcggcac
    36181 gggttgccag acctttcccc tgatactccg gggagataat tatctgaaat tctgcccggc
    36241 gatgtacatg gttaatttcc accagctcca ccagaccggc tttttcgccg tcacattcca
    36301 ccacaaagcg tcgttcgctc tgatcgtgaa tatgcttatc atacagatca gagagttcaa
    36361 caaaggcttc gtagggttcc tcaaaccagt aacgcatcac actggcgtta ttgtcgagtt
    36421 gatgtacata gcgtaaatct tcacgctcca gcgggcgtag cttaacactg tgggcgcttg
    36481 gcataacgtg tccttacatt ccttaaatca ataacaggtt agggggtaat aacgcggcca
    36541 gttcgacggt ccaggcagcg caaagtattt ggctcccagt aggcattgat gttggcgctt
    36601 tgctcacatt tatcgcggtt atcaaaagcg gcgtcggctt tatcccactc tttttcagtg
    36661 cgtttattca ctttctgacg cagattgcgc gtgtcattcc attgctcttt ttccatagcg
    36721 gcgtgctggc ggctttgtgc actgtcgcca gactcaatca ccagtttgtt agtttcggca
    36781 tgaacagttg tgctcagtgc cagtgcgcaa ggcagcagaa aagcgagcag gccgattcgt
    36841 ttgctgagag tgattttcat aattcattcc ctgtatgaat gattaaagga gattctacac
    36901 catccactgc gaacgcaaaa cgtaccagga gggtgtttat attgatgata ttatgtcgcc
    36961 ctataactat acatgatgtc aataagtgac aaagatgatt aaaacaacgt tactattttt
    37021 tgctactgcg ctgtgtgaaa ttattggatg ctttctgccc tggttgtggt taaaacgaaa
    37081 cgccagtatc tggctgttgc ttccggcggg gatttcactg gcgctgtttg tctggttgtt
    37141 aacgttgcat ccagcggcga gtgggcgtgt ttacgcggct tatggtggcg tttatgtctg
    37201 cacggcgttg atgtggctgc gcgttgtgga tggcgtgaaa ctgactcttt atgactggac
    37261 gggtgcgttg attgcgcttt gcggcatgtt gatcattgtt gcgggctggg ggcgcacgta
    37321 ggaacataaa tccattttat caataagata agaggaagtg tcagctgaca aaaggtattc
    37381 tatttcatct tttgtcaacc attcacagcg ctaatatacg cctttttttg tgatcactcc
    37441 ggcttttttc gatctttata cttgtatggt agtagctcag ttgcgtagat ttcatgcatc
    37501 acgacaagcg atgcaaggaa tcgaacatga agatcgtaaa ggctgaagtt tttgttacct
    37561 gtccggggcg taatttcgtc acattaaaaa tcaccactga ggacggtatt acgggccttg
    37621 gggatgccac cctcaatgga cgtgagcttt ccgtggcctc ttatttgcag gatcaccttt
    37681 gtccgcagct tattggtcgc gatgcgcacc gtatcgaaga tatctggcag tttttctata
    37741 aaggtgctta ctggcgtcgc ggtccggtta cgatgtcggc catttcagcg gttgatatgg
    37801 cgctgtggga tattaaagcc aaagctgcca acatgccgct ttaccagtta ctcggcggcg
    37861 cgtctcgtga aggggtgatg gtttattgcc ataccaccgg tcacagtatt gatgaagctc
    37921 tggatgatta tgcccgtcat caggagctgg gattcaaagc catccgcgtg cagtgcggaa
    37981 tccctggtat gaaaaccacc tacggcatgt cgaaaggtaa aggtctggct tatgaacccg
    38041 caaccaaagg acagtggccg gaagagcagt tgtggtcgac ggagaaatac ctcgatttca
    38101 tgccgaaatt gtttgacgcg gtacgtaaca agtttggttt taatgaacat ttgctgcatg
    38161 acatgcacca tcgcttaacg cctattgaag cggcgcgctt tggtaaaagc attgaagatt
    38221 atcgcatgtt ctggatggaa gacccgacgc ctgcagaaaa ccaggaatgc ttccgtctca
    38281 ttcgccaaca taccgtcaca cccatcgcag tgggtgaagt cttcaacagc atctgggact
    38341 gcaaacaact gattgaagag caactcatcg attatatccg caccacgctg acccatgcag
    38401 gcggaattac cggtatgcgc cggattgccg attttgcttc gctgtatcag gtacgtactg
    38461 gctcacacgg tccttccgat ttgtcaccag tctgcatggc tgcagcgctg cactttgatc
    38521 tgtgggtccc caatttcggt gtccaggaat acatgggtta ttccgaacaa atgctcgaag
    38581 tcttcccgca caactggact ttcgataacg gctatatgca tccgggagac aaaccgggtc
    38641 ttggcatcga attcgatgaa aagctggcgg cgaaatatcc ctatgaacct gcttatctac
    38701 cagtcgcacg tctggaagat ggcacgctgt ggaactggta aggagtaaga taatgaaaag
    38761 catattaatt gaaaaaccga atcaactggc gattgtcgaa cgtgaaatac ccaccccgtc
    38821 agcgggtgaa gtacgagtaa aagtgaaact tgccggaatt tgtggttcag atagccatat
    38881 ttatcgtggg cataatcctt ttgcgaaata tccgcgcgtc attggacatg aattctttgg
    38941 cgtcattgat gcggtgggtg acggcgtgga aagcgccaga gtcggtgaac gtgttgctgt
    39001 cgatccggtg gtcagctgtg ggcattgcta tccgtgctct ataggtaaac cgaacgtttg
    39061 tacgacactt gctgtattag gtgtgcacgc tgacggtggt ttcagtgaat atgccgtggt
    39121 tccggcaaaa aatgcgtgga aaattcctga agcagtggcc gatcaatatg cggtgatgat
    39181 cgaacctttt accattgcgg ctaacgtaac cggacatggt caaccgactg aaaatgatac
    39241 cgttctggtt tatggtgccg gtccaatcgg cctgacgatc gttcaggtat taaaaggcgt
    39301 ctataacgtt aaaaatgtga ttgttgccga tcgcattgat gaacgactgg aaaaagcgaa
    39361 agagagcggg gcagacaggg cgattaataa cagccagaca ccgcttggcg agattttcgc
    39421 tgaaaaaggc atcaagccga cattaattat cgatgcggct tgtcatcctt ctatcctgaa
    39481 agaggccgta acgctggctt ctccagcggc acgtattgta ttgatgggct tctccagtga
    39541 accgtctgaa gtgattcagc aaggaattac cggaaaagaa ctctctattt tctcttcacg
    39601 cttaaatgca aataaatttc cggttgttat cgactggtta agtaaagggt taattaaacc
    39661 agaaaaatta attacccata cgtttgattt ccagcatgtt gctgatgcca ttagtttatt
    39721 tgaacaggat caaaaacatt gctgcaaagt cttactcact ttttccgaat aataccaata
    39781 acggcgagta agtagtacgc atcttacctc ttttttagag ataaccatta tgacaataga
    39841 aaaacatgaa agaagcacta aggatttggt gaaagcagca gtatcgggat ggctgggcac
    39901 tgcgcttgaa tttatggatt tcaagagtca tgcgtgttaa ctatttgata aatattaaat
    39961 taatttttca ttgcttcgtt atggggcatg gttggggcaa actcgcttaa ctgtgtattt
    40021 aacaaagcta cctgtgcatt attgttttca gacatccatt ttccgtatac ctgaaatacc
    40081 atttgcgcat ctgcatggcc catctggttt gctataaatg ccgggttagc accagctgtc
    40141 agcgaccagc aggcataagt atgtctcgac tgatatgatt ttcgatggcg gagtccggca
    40201 cgttttatcg ctgcgtccca catctgcctt attgagtcaa cggtaaaatg gtcaccataa
    40261 ttttttactc tcgctgacac ttcaggttga aaaacaaagg tgcatttttg tttttctgtt
    40321 ctgccatact ctctgaggtg aacatcaatg atatgctctt tgctcagtct cgttaatgtc
    40381 atctgactcc ggagagcgtc gattgctggc ttaataagat gaatgacccg attggttccc
    40441 gcctgtgttt ttggtaccgt gaaacggtct tttgctaaat ttctcctgat catcattgtt
    40501 ccatttttca gatctatgtc ctcccatcca agtgcacaca gctcaccagg gcgaacgcca
    40561 gtataaacag aaacacacca taaatttttt gcttgctgat ttctgcacgc atcgataaga
    40621 cggataaatt cttcccgcga aagaggatcc ggaatggttc ttgattcctt taatggcgag
    40681 atccccttaa acggattatc tgccaggtaa ccgttatcaa caccaaactg gaacacggcg
    40741 ttaagatttg tcatgtaatt atttacagtt acagccgatc tccctggttg tgtaacaata
    40801 tagttacttt tggggatctg gtatccagtc agtaactctt tacgagcctc cagtaatttt
    40861 tctttattaa tcgatgaggc aagatttttt tcaccgatta tgctcaggat atttttgatg
    40921 acggcacggt atgtgttgag tgatgttttg gcgacttcag tttctttcag tgccagaaat
    40981 ttttcagcca gttcttttat ggttaaatct tgtcgggcct caccaaattt ttccagattg
    41041 cgtgaggagg gaaactgttt tgcatagtcg aaaacaccag tttttattgc gtaacaaaca
    41101 gaggagcgta gttcacctgc aacgcgcctg ttttttgctg tgtcaggaac ccccagattt
    41161 tccctgactc ttacgccttt ataaacaaac cagatacgta atttccctcc atggttttcc
    41221 acgcctgtcg gatatttcat ttcaacttct ctcattagtt agtgtggctt ttagtcaagt
    41281 aagatgacgt cttggtctcg ctgatgcctg gcgctcaatc cagcgatcaa tttcttccag
    41341 gttgtaaaag catggactgt tatcccatgg cataccgtca tgagcgacat gcttatattc
    41401 ccttccttcc ataaacgatt tttcccgggc cttttttaac gtaccttttt ttattccttt
    41461 cagcgcaatt aactgctctt cggataccca tttgccggga gagacaatca tgattacttc
    41521 gctcatcgat ttctttatct cttacatcag acgagcgccg gttgcagaat accagtcaca
    41581 accggcgaca gttgaacatt aagaatcagc ctgactcggg atcagttttt gccagataac
    41641 tgaaacgtat tttgcctggt aacgggcgtc atcaagtgca ttatggcgct caccttcgaa
    41701 tggaatagcc gttctggcat cgaagtctat ggctttcccc agctcaacga ttgtgcgtac
    41761 atcgcgatcg ttgtagtaac gccacgggca ggggatcccc tgccgctcgt atgaacggcg
    41821 caaaatcgtg ttgtcgaagt tggctccatt tccccagacc tgaacaaaaa attcaccgga
    41881 gttttcgtcg ataaattccc gcaattgtaa cagtgcatca tctaacggga tttcatcggt
    41941 cataatggca gattgcgctt cgcgtgattg cttaagccac catttaatgg tgtcccgatc
    42001 aatgactccg ccagcagttt ccagatcgat agtcttacta aattccggtc ccatatctcc
    42061 ggtttgcgga tcgaaaaata ttgcacctat tgagatgatc ggggcatcag gattttttcc
    42121 catggtttca aggtcgatca ttagatggtc acacgtcctg ctggtggatg tgatttcgtg
    42181 atgaccgttc accttaattg agcgatctgc cgtctcgcca gtttcattat cgctatcgtg
    42241 atgctgattg ccgccagtgt tctccttgtg tggatgttca gcgccttcca tttcctccgg
    42301 atcatcttcc tgaacttcaa cctgatactc ttcatcgaat gtttcctggt atgttgcgtc
    42361 gcccatcacc gcgccacaat cagggcagtt gccgccgccg gtctgaccgc aggcggtgca
    42421 gactttttcc acttcctgtt gcgccactgg ttcaggctgt ttcgtttctg gctcgttttg
    42481 taacgcattt gggctgtttt gttccgcttt ttggtagttc cgttccgatt catgctggtt
    42541 ctggttcaca gaatcgcggg tctggatccc cttaacccat ttcggatcat tcgggtcact
    42601 aatcccttca acaaattcac cacgtgatgc agcaagcaac ttatcggcgt caggctggct
    42661 gatattggct gcctgcataa ttttgtttac ttcgtcagcg gtaactttta tcggctctgg
    42721 ttgttctgaa tcttcagcgg tatctacatt ttgcggtaag cccgtgtatg tgccattttt
    42781 tcgggcaaaa tattcttctt ttgtgatttc agtggcgcca gcagccagtg ccttatccag
    42841 accagaaagt ttgtttgcgc gaccgtattt ttctccgtcc ttatctgcga agaggaaata
    42901 gaacggcccc tcacgctcta cagatggttc agcttccggc gcggtttcat tttttgggat
    42961 atcagatacc tcggtttcca ctgcatcagt ttgtgtttct gatgactgga gaacatcaac
    43021 agtgcccggg tctgtttctt cattctcaaa cacgcccttt gtcgtcaggt attcgcagat
    43081 atatttgttc agtgctacgg gatctttgtg aatgtcgatc ggacgctcac ggacaaggcc
    43141 aaaaatagtt tggcggtcgt agcgaagggc atcaggctgt ttgcgcattg atgccgagat
    43201 acgcttccag tcttcgcggt cgttgtcgat aacttctttt tttgcccagc gatggatgct
    43261 gccgtcaatg tttccggcat ccacatcacc aggccagaga gcgtaggcca gttcgtcatc
    43321 cagtgttttc catgtctgct tgtattcgcg atgaatggca gcaatgaccg ggctgatttt
    43381 tcctgttgaa ttttcagtgt actgttgatt ggctttggcg cgggcgagat caacaacaga
    43441 cgtgtatttt ccggtttcct tgcgttcacc ttcgcgacgt tttttccaga tgcgcatctc
    43501 tgcctgaatt tcgggccatt tagcaccagg aatacattta tgcttaaccc acccaatggc
    43561 gtgcaactta agctccggat acatggcgtt aacttctggc attttcatca acgcttcaac
    43621 gatatgtccg tcgaatgttg ccatgtcttc ctgcaacaat tcctgtgcgc taataaccat
    43681 atcaacggtg atgttttcac atgtgtcgaa cttaaccatg acagcgttct gtacttcagg
    43741 ggccagcttg tcaaaagtga cgttcatcgg atcggattca gtctcaaccg ggacaaaaga
    43801 agcagactcc tcatcccagc ggttttcctg catatattca gcatcccatg aatcgagggc
    43861 agggcggggt atgccaggtt tatcctcgca gacaataaat ttataagcgc agtcctgagc
    43921 agccggataa tgttccagga actgccagtg aaattttgct cgagcacggc gttcgtcgcc
    43981 agcttcaatg gctgtggcta cagccacagc cacagcgcct tcttcccttg ttgccagttc
    44041 gtcaggaata gcggcgcaaa taaagacttt actcattttg ttttaacctc atgacagatt
    44101 taaggatgaa caaatccctg ccattgctgg catataagaa tgaaaccgga tatttattac
    44161 ggaactgttt taaagacctg ccgggatttc gatattatcc tggtgaataa ctttatcgac
    44221 cgggtaacag ttaccgggaa ttttctgttc ggttgctgca gtcatacact cctgcattgt
    44281 cctgtgaaca ctgactgcaa tatcaactgg ctctccggaa acaagaaaaa ctgtcagaac
    44341 aagcgcaaat gctgaattca ttgtgcacat ccttttggca tcagacgtaa acgagccagc
    44401 attgaaacaa tgcatatttt atttaatagc tcccgttctt gttttctctt gttaatggca
    44461 tcttcagtaa atacagggtt actgatagtg acaccaattt caaaacaacc ttcagacgta
    44521 ttaacgtttg gtaataacgt tttcattatc gcgtcctcaa caatgaattt tgtgatgcag
    44581 tgcctggtgc ctccaggtga cgttaaccag ttaacaatta acgccggata cagagaatcc
    44641 acccataaca ctgtttttgg ttttaactgt tccgcgtgcg cttagccgca ttcaccgcat
    44701 cacaaaattc actttaaaaa cggcggtaga gcagtcacgg agtaaaactg atactgccaa
    44761 acgtcaccag aaaattgata acagagggcg ttgcagcggg gttgtcactt aagcgtatgg
    44821 tcaacctgac aactcggtgt cctcaacagg gaaggaataa ccccgccata cttaccgccg
    44881 cgccatttcg cgggttgcca caaccggaag cgcacggtcg aattaaattt aacgacaccg
    44941 tacagtgaga cgaacttcgc cgtgcgcttt cgtgttgtgt gcctgctttt aaccacgtca
    45001 ggcgaggtgg tatccttaaa atcaccacag ttttaaggat tcattaagca atgtcgcaac
    45061 caccaataaa tccgcttaag aacatgaaaa ttgattactg gtataaagcg cttacagtta
    45121 ttggcgctgc gttgtttgtc tttaatggaa cgtctttttt tgacagatat cccgttgttc
    45181 cattgggttt tttgtcctcc ggcatctttt ttattggttt gggggagtgg attaatcacc
    45241 ctctcaaggt gagatttatt ggtcctggag tttggactcg tggatataat cgttcttcgt
    45301 gcgcactcgg tatcatcttc gacatacttg gttgtttcct gattgttaca ggagtcgtca
    45361 agttcttctg atgtaaaacc gcaaatgggg cacgtaacgg gaattttgaa aagcgtttct
    45421 ccgggttcca gaacaaaatt ttctgcggtc tgattttgct tctcatattt gtgctccgcg
    45481 tcattgtgag agcacattct tattctgagt gcctgtttaa actcactgaa gctgagagct
    45541 tcttcgcctt cggcaaggcc ttcgaagtat tcttcgtaag ccttttccat gattgtgtcg
    45601 aaatccatat cactcacctg agtttctttc cagccagcga cgggcaccat tttcggtttt
    45661 aaacgttttg cttttggtat acgtcatcgc ggtgaacgtg ccgtcctggt tggggaacac
    45721 gccacatacc agagattcgc tgttgccaag atcgatagta tccatgctga cctcatttcc
    45781 ccttaacgcc ggggtggcgg aactgtttgc tgagaacacc gtgcggtgtg ttgatgtaaa
    45841 caagattagc catgactaac atattggtca agtgattttg tatgttatgg ctaacataat
    45901 tgatataata aaagataact cattgatgat gttatctttt atttgtccgc tgacgggctt
    45961 ttagtaattc ttcaaagagt ttattgaaat tttttactcg agctcgcatt tcggcgagct
    46021 gggtatcctg ttctgattct ggcagtgcat taaacagctc aaggagctct agttctttgg
    46081 gggctaaggc aaccggcttc tcaacaggtg gtgttggttg cttgtcttca tcgccaaata
    46141 gaatccatgt tggtgagcat tgcaatactt tgctgagggc aaaaaggttc ttccctgtag
    46201 gttcactatc atcccgttcc cattgtgata cagacacatg ggagattttc agggctttag
    46261 caagagacct ttgggtgtgt ttgaggtttt tccgacgata cctaatgcgt tcgccgatgg
    46321 ttaaattttt tgtatccata gttagctaat gctaaatcgt attgactatg tttttgttaa
    46381 catctatttt gttagtcatg actaacatct aaggtgtttt aaatgcttaa aactgacgct
    46441 cttttgtatt tcggttcaaa aacaaaactt gcacaagcag caggtattcg tttggcttcg
    46501 ctttatagct ggaaagggga tttagttccc gaaggtcgcg cgatgcgtct acaggaggca
    46561 tctggcgggg agcttcagta tgatcccaaa gtttatgacg aatatcgtaa gacgaagcgg
    46621 gcggggcggt tgaacaatga aaatcactcc tgaacaggct cgtgaggctc tggatgcctg
    46681 gatatgtcga ccaggaatga cacaggagca ggcgacgata ttaatcactg aagcattctg
    46741 ggctttgaaa gagcgctcga acatcgatgt tcagcgtgtc acatatgaag gtggcgcggt
    46801 tgatcagcga gcgcttagcg ttaatcgagt gaagatattt gaacgctgga aggctatcga
    46861 caccagggat aagcgtgaaa agttcacagc gctagtgcct gcaattatgg aggctatccg
    46921 gattagtgat ttcaggttgt atcgtgaaat tagtgacgga aagagcatta cgtacatgat
    46981 cgctgggtta aataaagaat atggcgatgt ggtggagtcc ggactgcttt ttgcagatcc
    47041 tgccgttgta gatcgtgaaa ctgacgaact tatagaaaaa gcaattgctt tcaagcttgc
    47101 gtatcgacag caataccaac aaaaagctgg atggaattat gagtcttctt tttgctgaac
    47161 gcccactggt tataaacacg cagctggcaa tgaaaatcgg cttaaacgaa gccattgttt
    47221 tgcaacaact gcactactgg ttgagagata ccaactccgg tatggaatgt gatggtgttc
    47281 gctggattta taacacaacg gaacaatggc tggaacagtt cccattctgg tcagagtcaa
    47341 cgttaaagcg cgcgtttgca agtctgaaaa cgctggggct tttgcgttgt gaaaagctca
    47401 ataaatcaaa gcgcgatatg accaatttct acacgatcaa ctatgggagc gagcttttag
    47461 atggtggcaa attgaacgaa tccatcggtt caaaatgcgc cgctccatca ggtcaaaatg
    47521 acacgatgga agaggtcaaa atgaaacgct ccattggttc aaaacgaccc aatgtcatcg
    47581 ggtcaaaatg gcctgatgat cttacagaga atacaacaga gattactaca gagaataaaa
    47641 acacttttcg tccggaagct tcgcaaccgg acccgcagac gactgaacag gattttttaa
    47701 cccggaaccc cgacgcggtt gtgtttagtg tgaaaaaacg ccagtggggt agcagggagg
    47761 atttggcgtg tgcgcagtgg atttgggggc ggatcgtgaa cctttacgaa caggctgcca
    47821 gcgacgatgg agagatcatg cgaccaaaag agcctaactg gacagcctgg gccaatgacg
    47881 tgcgcacaat gcggatgctg gatggcagaa gccatagaca aatttgcgaa atgtttggtc
    47941 gggtacagcg ggatccattc tgggtaaaaa acatcatgag cccgtcaaaa ctccgcgaaa
    48001 aatgggacga actggtcatc cgcctgggac gttcacctga acagcgttgt gtgaatcata
    48061 tttctgaacc ggatacagaa attccgcctg gtttcagagg ataagttttg atttcaggtc
    48121 atgaggtaat tttaaggggg acttgtggca aaagttttta cacaagaaga gcgggaaaaa
    48181 attaaagggc aggtggtgga actcgtgcgc cagagtggtc gtgagacgtt acggcaactg
    48241 gaagctaaaa caggtgcgac tagatatctg atgagcgttc ttgccagaga gctggtagcc
    48301 agtggcgatg tatacaactc cggctacggg ttattcccgt ctgaacaggc gcgtaaggac
    48361 tggcaaaacg cccgcaaaaa actatcgagg gcaaaggtga agaaaacagc tgtggttgat
    48421 ccggacctta tctggtcgtt acctgatgga gaaatacgtc gctacgacag tcgcctaaac
    48481 ataatctgtc gcgagtgccg gatgagtgaa gttatgcagc gcatactggc attttatcag
    48541 ggataatgtt aggtatttta gacgttacta gattaaaaag cattagttca ggagtgaatt
    48601 gacattctca tttttcatgg cacagggtag atctggcgtg gttgtccgct ttgtgccagg
    48661 agcggacgtt gctaatggca tgatgtatga accaatggag aaccgatcat ctatacacat
    48721 atagatccct aacgaaaata ttgttttcac tagccctgta atggaagcgt taattttata
    48781 ttctaacgct tccattgagc ctaccacttt ggcaaagcaa ttcttacact tcaaaataat
    48841 gtgctttttt attcagctaa ataaccgcca tcgattggat aaacgcctcc ggtacagaat
    48901 gtagaactgt ctgacagcaa gaacaatatg aagtctgcta tttcctgcat ttttgccatc
    48961 cttttcatag ggtggctatt tgcgaaacta cttacaatat tttcagggaa ctcactcatt
    49021 ctttgggttt ggacatagcc cggagcgact gcattgattc gaatgccttg acatgcaaac
    49081 tctagcgctg cggtttgggt taaaccgatt ataccatgtt tagccacggt gtaaggtgct
    49141 aatccaggaa tgccaaccag accatttact gcagataaat taactataga tccaccaaac
    49201 ttcatcatct ggggtatttc atattttagg cagtgaaaaa cgccgctcaa tgaggtagca
    49261 ataaccttat cccagttttc aactgtctgc tctgttatat ttttaccatg ttcacctgtt
    49321 aagcctgcat tatttacagc ataatcaagt ctgccgaaat gtttgataac ctcatctatc
    49381 atttgccgta cttgttcggg ctgtgacaca tcacatccga tccccatggc ttgttgtccc
    49441 tgtgaagata attgttccgc tttttgttta gcctgttctg acgagcggga tacaataaca
    49501 acttttgcac catgcttatg taattgctct gcgacggcct cacctatacc tgtagtgctt
    49561 cctgttataa cggcaatttt attctggaaa ttcataacca ccccctatga agtagaattg
    49621 agtctttcat ctgggatagt atacaaacca tctgtgacag tttttgtcag tactctttcc
    49681 cctcgcagat cccttctttt attctccatt ttttcaataa aattgtcctg ctttcactat
    49741 agttggagtc aagttgcaca atatcttcaa ttttgtcagt tctaaaatga cgaaaatcat
    49801 ttctcatctc gcaccatgca accaaaagcc agcacgattc catgtatacc aatccgatgg
    49861 gccatatgac tcgtgaagtg taagaatctt ttttatcaca ataatttatt ttgattttct
    49921 ttcttttttt tatagcattg cgaatgtctt caaaaaaaat ctcattgttt tctgatggac
    49981 cgatcagata ggattgactc tcaataagat ttttcaattc actgggaatg acggcgtgta
    50041 ttttcgcaag cgcattcttt gcggtaattt tgaaattata atctgtattg tgcgataccc
    50101 aatttaaccc aagtgtaata gcattaattt cttcatgaga aagatttaag gggggcaaat
    50161 gaaagtcaga tttaacaata taaccaattc cagcaccacc ttcaatgcat actccttgat
    50221 gctgcagcgt ttttatatct ctgtaaaggg agcgtacact tattcccaat ctttctgaga
    50281 ggatggatgc tgtgatcgga tatctttgtg cccttaatat ttgtagtaac tcgagaagtc
    50341 tttctgttct agtcatgttg ataaatccat tttgaggcga tttcttaaag catctgttag
    50401 ataaacatat tagtacaaag caagtgtaca tggctaagct gaactgctac cgatgattaa
    50461 tacagcgcta tgttcgtaat gcgtacataa gcggtgatca tgagtgtaaa catccgcttt
    50521 tcgcttataa gggacaacca tactcaaatt tcccacattg caggagattt gagtatgaat
    50581 acgtcaccgt ggaacaaaga ccgtatcatc ggccaaaaaa gaccacttca gatatctcat
    50641 atctggggga tccgaatccg gcttgaactg gaaggtaaaa cgcgcgattt agctctgttc
    50701 aatatggccc tggacagtaa gcttcgaggc tgtgatctgg tcaaactcaa agtatcggat
    50761 gtggcatatg gtagttcggt ttcaagcaga gcaacggtgt tgcaacagaa aaccggtagc
    50821 cctgtgcaat tcgagataac caaagggaca agagaagctg ttgctgcatt gataaagctt
    50881 ggcaatttgc acagtaaaga cttcttgttt cggtctcggg tcggaactaa ccagcacatt
    50941 tcaacccggc aatacaaccg aatctttcat gggtggatag aaaaacttgg tctcgaaggt
    51001 tcactttaca gcacacattc aatgagaaga acaaaacctt acctgatcta caagaaaacc
    51061 aagaatctcc gggtgatcca acttctgttg ggtcataaga aactggaaag cacagtccgt
    51121 tatctgggca ttgaagtcga tgatgcgtta gagatctctg aatcgattga agtctaaggt
    51181 tgtcagggct gcaacagcag ccctgtgcca ggagcggacg tctagttcgc tgaaccacaa
    51241 aaagtcgttt ctagttatat tactggctca ttagtatctt tatgcgataa tatttcatac
    51301 aaccaaaatt atgatgaagg gagttcaaat ggcaattacg gtctattctc gtacattcaa
    51361 aaaggaattg gacatggttc aattgttaaa ccattactca tcagataacg cactcaattt
    51421 cagtgatttc aaggaatttg ttgcctttga tgcagagtgc ccaatttgca atgtaagcgg
    51481 tgcgattgtt gtttcagaag gttattcgaa aagaacaaat aagatagtca gccaatctca
    51541 ttttgcgttt agaacaaaag acggaactga tgcacacaaa gtattttgcg atcattacag
    51601 tggtgatgat aagcaaatag attcatcaag ggatggtttt ataaaattcg gcacctcagg
    51661 ttctgagatt acaagtataa ttcgcgagct tgtatgtaga ggtattgaac atgatgagtt
    51721 caatcaggaa gatattcgaa acatgcggca atggtttaca gaattacgcc agtcaggcac
    51781 tttttctgtt aactatagcc ctcacatgat caatctaata agggccagca tgtattcccg
    51841 taagggagaa agtaaatata ttgtagatga agagaggaaa aataaatctt ggtttgatat
    51901 aaacgatgaa gtgtatcgtt cgttaagatt caaatatcca tcattgatga tcgacatgac
    51961 taatgaaaat aattctgttt tctataatct tgtgaatagt accacattta ctaaacaagc
    52021 acatcgatta gtagcacgag atcgaggtta tcaggtttat gatagaaggt tgcttaaaga
    52081 aaaatatgaa gcaacgataa gagttgcaca aaaaataacc aggcatcacg aatttctttt
    52141 taggaaaatc aggggtatat ccgctgttaa aaaaaacaac ccccttttgg ctgtatctgc
    52201 cttgcttctt tttgtctcgg actggaatga aacaactgca atcaacaaat tcatcaaatt
    52261 gtgtggtgta gggaaatcta aaaataatga tgaggggaat gtaattggct taaatccctt
    52321 tattcattac gatgtctgga atgttattca tcaacttaag gatttaatgg ttacacttcc
    52381 agacttctca aatcttgatg aagaatttga cagagaaaaa tccagattaa ttgaacttta
    52441 caagctctaa cataaactag caggctgcgt atacagtctg ctaattttca tagtaaagac
    52501 ttcatgcttc agtctcgagt tggagctatc cgtcacacat cacccaggca attcaaccga
    52561 aatttttcat ggggggtaga gaagttttgt ctcgaagatt tgctttacag catactaatc
    52621 attgaacctc tgttgaacca taagaaactg gcaagcacaa tccgttgctt gggcattgaa
    52681 ttcgacgatg cgtcgtatag ctctgacttg aacaaaacat aaggttttta gggctgcaac
    52741 agcacctctg tgtcataggc ggaagttaac aaacattaaa atctctaaat ctgtagggat
    52801 cagactcctt ctataaaagg atactgttca tatccttagt tgta
//
LOCUS       DAZGAN020000021        52644 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGAN020000021 DAZGAN020000000
VERSION     DAZGAN020000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 52644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.021"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            27..344
                     /locus_tag="ACHKYO_RS07165"
                     /old_locus_tag="pgaptmp_001433"
     CDS             27..344
                     /locus_tag="ACHKYO_RS07165"
                     /old_locus_tag="pgaptmp_001433"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HGU4428302.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            413..742
                     /gene="yjdP"
                     /locus_tag="ACHKYO_RS07170"
                     /old_locus_tag="pgaptmp_001434"
     CDS             413..742
                     /gene="yjdP"
                     /locus_tag="ACHKYO_RS07170"
                     /old_locus_tag="pgaptmp_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HGU4428303.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(889..1647)
                     /gene="phnP"
                     /locus_tag="ACHKYO_RS07175"
                     /old_locus_tag="pgaptmp_001435"
     CDS             complement(889..1647)
                     /gene="phnP"
                     /locus_tag="ACHKYO_RS07175"
                     /old_locus_tag="pgaptmp_001435"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HGU4428304.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSSQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(1649..2083)
                     /gene="phnO"
                     /locus_tag="ACHKYO_RS07180"
                     /old_locus_tag="pgaptmp_001436"
     CDS             complement(1649..2083)
                     /gene="phnO"
                     /locus_tag="ACHKYO_RS07180"
                     /old_locus_tag="pgaptmp_001436"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HGU4428305.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(2070..2627)
                     /gene="phnN"
                     /locus_tag="ACHKYO_RS07185"
                     /old_locus_tag="pgaptmp_001437"
     CDS             complement(2070..2627)
                     /gene="phnN"
                     /locus_tag="ACHKYO_RS07185"
                     /old_locus_tag="pgaptmp_001437"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HGU4428306.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(2627..3763)
                     /gene="phnM"
                     /locus_tag="ACHKYO_RS07190"
                     /old_locus_tag="pgaptmp_001438"
     CDS             complement(2627..3763)
                     /gene="phnM"
                     /locus_tag="ACHKYO_RS07190"
                     /old_locus_tag="pgaptmp_001438"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HGU4428307.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            complement(3760..4440)
                     /gene="phnL"
                     /locus_tag="ACHKYO_RS07195"
                     /old_locus_tag="pgaptmp_001439"
     CDS             complement(3760..4440)
                     /gene="phnL"
                     /locus_tag="ACHKYO_RS07195"
                     /old_locus_tag="pgaptmp_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HGU4428308.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(4617..5375)
                     /gene="phnK"
                     /locus_tag="ACHKYO_RS07200"
                     /old_locus_tag="pgaptmp_001440"
     CDS             complement(4617..5375)
                     /gene="phnK"
                     /locus_tag="ACHKYO_RS07200"
                     /old_locus_tag="pgaptmp_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HGU4428309.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(5372..6217)
                     /gene="phnJ"
                     /locus_tag="ACHKYO_RS07205"
                     /old_locus_tag="pgaptmp_001441"
     CDS             complement(5372..6217)
                     /gene="phnJ"
                     /locus_tag="ACHKYO_RS07205"
                     /old_locus_tag="pgaptmp_001441"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HGU4428310.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(6210..7274)
                     /gene="phnI"
                     /locus_tag="ACHKYO_RS07210"
                     /old_locus_tag="pgaptmp_001442"
     CDS             complement(6210..7274)
                     /gene="phnI"
                     /locus_tag="ACHKYO_RS07210"
                     /old_locus_tag="pgaptmp_001442"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HGU4428311.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(7274..7858)
                     /gene="phnH"
                     /locus_tag="ACHKYO_RS07215"
                     /old_locus_tag="pgaptmp_001443"
     CDS             complement(7274..7858)
                     /gene="phnH"
                     /locus_tag="ACHKYO_RS07215"
                     /old_locus_tag="pgaptmp_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HGU4428312.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            complement(7855..8307)
                     /gene="phnG"
                     /locus_tag="ACHKYO_RS07220"
                     /old_locus_tag="pgaptmp_001444"
     CDS             complement(7855..8307)
                     /gene="phnG"
                     /locus_tag="ACHKYO_RS07220"
                     /old_locus_tag="pgaptmp_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HGU4428313.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETVIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(8308..9033)
                     /gene="phnF"
                     /locus_tag="ACHKYO_RS07225"
                     /old_locus_tag="pgaptmp_001445"
     CDS             complement(8308..9033)
                     /gene="phnF"
                     /locus_tag="ACHKYO_RS07225"
                     /old_locus_tag="pgaptmp_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HGU4428314.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(9054..9833)
                     /gene="phnE"
                     /locus_tag="ACHKYO_RS07230"
                     /old_locus_tag="pgaptmp_001446"
     CDS             complement(9054..9833)
                     /gene="phnE"
                     /locus_tag="ACHKYO_RS07230"
                     /old_locus_tag="pgaptmp_001446"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HGU4428315.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(9939..10955)
                     /gene="phnD"
                     /locus_tag="ACHKYO_RS07235"
                     /old_locus_tag="pgaptmp_001447"
     CDS             complement(9939..10955)
                     /gene="phnD"
                     /locus_tag="ACHKYO_RS07235"
                     /old_locus_tag="pgaptmp_001447"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4428316.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(10980..11768)
                     /gene="phnC"
                     /locus_tag="ACHKYO_RS07240"
                     /old_locus_tag="pgaptmp_001448"
     CDS             complement(10980..11768)
                     /gene="phnC"
                     /locus_tag="ACHKYO_RS07240"
                     /old_locus_tag="pgaptmp_001448"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HGU4428317.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(11901..12344)
                     /gene="yjdN"
                     /locus_tag="ACHKYO_RS07245"
                     /old_locus_tag="pgaptmp_001449"
     CDS             complement(11901..12344)
                     /gene="yjdN"
                     /locus_tag="ACHKYO_RS07245"
                     /old_locus_tag="pgaptmp_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HGU4428318.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(12504..12839)
                     /gene="yjdM"
                     /locus_tag="ACHKYO_RS07250"
                     /old_locus_tag="pgaptmp_001450"
     CDS             complement(12504..12839)
                     /gene="yjdM"
                     /locus_tag="ACHKYO_RS07250"
                     /old_locus_tag="pgaptmp_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGU4428319.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            13241..15469
                     /gene="crfC"
                     /locus_tag="ACHKYO_RS07255"
                     /old_locus_tag="pgaptmp_001451"
     CDS             13241..15469
                     /gene="crfC"
                     /locus_tag="ACHKYO_RS07255"
                     /old_locus_tag="pgaptmp_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HGU4428320.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSAVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            15466..16343
                     /gene="rdcB"
                     /locus_tag="ACHKYO_RS07260"
                     /old_locus_tag="pgaptmp_001452"
                     /pseudo
     CDS             15466..16343
                     /gene="rdcB"
                     /locus_tag="ACHKYO_RS07260"
                     /old_locus_tag="pgaptmp_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
     gene            16607..18109
                     /gene="proP"
                     /locus_tag="ACHKYO_RS07265"
                     /old_locus_tag="pgaptmp_001453"
     CDS             16607..18109
                     /gene="proP"
                     /locus_tag="ACHKYO_RS07265"
                     /old_locus_tag="pgaptmp_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HGU4428321.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            18221..18310
                     /gene="pmrR"
                     /locus_tag="ACHKYO_RS07270"
                     /old_locus_tag="pgaptmp_001454"
     CDS             18221..18310
                     /gene="pmrR"
                     /locus_tag="ACHKYO_RS07270"
                     /old_locus_tag="pgaptmp_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HGU4428322.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(18286..19386)
                     /gene="pmrB"
                     /locus_tag="ACHKYO_RS07275"
                     /old_locus_tag="pgaptmp_001455"
     CDS             complement(18286..19386)
                     /gene="pmrB"
                     /locus_tag="ACHKYO_RS07275"
                     /old_locus_tag="pgaptmp_001455"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HGU4428323.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19387..20055)
                     /gene="basR"
                     /locus_tag="ACHKYO_RS07280"
                     /old_locus_tag="pgaptmp_001456"
     CDS             complement(19387..20055)
                     /gene="basR"
                     /locus_tag="ACHKYO_RS07280"
                     /old_locus_tag="pgaptmp_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HGU4428324.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(20052..21695)
                     /gene="eptA"
                     /locus_tag="ACHKYO_RS07285"
                     /old_locus_tag="pgaptmp_001457"
     CDS             complement(20052..21695)
                     /gene="eptA"
                     /locus_tag="ACHKYO_RS07285"
                     /old_locus_tag="pgaptmp_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HGU4428325.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(21799..23136)
                     /gene="adiC"
                     /locus_tag="ACHKYO_RS07290"
                     /old_locus_tag="pgaptmp_001458"
     CDS             complement(21799..23136)
                     /gene="adiC"
                     /locus_tag="ACHKYO_RS07290"
                     /old_locus_tag="pgaptmp_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HGU4428326.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(23273..24034)
                     /gene="adiY"
                     /locus_tag="ACHKYO_RS07295"
                     /old_locus_tag="pgaptmp_001459"
     CDS             complement(23273..24034)
                     /gene="adiY"
                     /locus_tag="ACHKYO_RS07295"
                     /old_locus_tag="pgaptmp_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HGU4428327.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(24359..26626)
                     /gene="adiA"
                     /locus_tag="ACHKYO_RS07300"
                     /old_locus_tag="pgaptmp_001460"
     CDS             complement(24359..26626)
                     /gene="adiA"
                     /locus_tag="ACHKYO_RS07300"
                     /old_locus_tag="pgaptmp_001460"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HGU4428328.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEVTPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(26825..27733)
                     /gene="melR"
                     /locus_tag="ACHKYO_RS07305"
                     /old_locus_tag="pgaptmp_001461"
     CDS             complement(26825..27733)
                     /gene="melR"
                     /locus_tag="ACHKYO_RS07305"
                     /old_locus_tag="pgaptmp_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HGU4428329.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            28016..29371
                     /gene="melA"
                     /locus_tag="ACHKYO_RS07310"
                     /old_locus_tag="pgaptmp_001462"
     CDS             28016..29371
                     /gene="melA"
                     /locus_tag="ACHKYO_RS07310"
                     /old_locus_tag="pgaptmp_001462"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGU4428330.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            29474..30895
                     /gene="melB"
                     /locus_tag="ACHKYO_RS07315"
                     /old_locus_tag="pgaptmp_001463"
     CDS             29474..30895
                     /gene="melB"
                     /locus_tag="ACHKYO_RS07315"
                     /old_locus_tag="pgaptmp_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HGU4428331.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(31034..31663)
                     /gene="yjdF"
                     /locus_tag="ACHKYO_RS07320"
                     /old_locus_tag="pgaptmp_001464"
     CDS             complement(31034..31663)
                     /gene="yjdF"
                     /locus_tag="ACHKYO_RS07320"
                     /old_locus_tag="pgaptmp_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HGU4428332.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(31786..33432)
                     /gene="fumB"
                     /locus_tag="ACHKYO_RS07325"
                     /old_locus_tag="pgaptmp_001465"
     CDS             complement(31786..33432)
                     /gene="fumB"
                     /locus_tag="ACHKYO_RS07325"
                     /old_locus_tag="pgaptmp_001465"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU4428333.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDKLPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRKGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(33510..34850)
                     /gene="dcuB"
                     /locus_tag="ACHKYO_RS07330"
                     /old_locus_tag="pgaptmp_001466"
     CDS             complement(33510..34850)
                     /gene="dcuB"
                     /locus_tag="ACHKYO_RS07330"
                     /old_locus_tag="pgaptmp_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HGU4428334.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(35421..36140)
                     /gene="dcuR"
                     /locus_tag="ACHKYO_RS07335"
                     /old_locus_tag="pgaptmp_001467"
     CDS             complement(35421..36140)
                     /gene="dcuR"
                     /locus_tag="ACHKYO_RS07335"
                     /old_locus_tag="pgaptmp_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HGU4428335.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQISGFQCCGTASTLEKAKEMIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(36137..37768)
                     /gene="dcuS"
                     /locus_tag="ACHKYO_RS07340"
                     /old_locus_tag="pgaptmp_001468"
     CDS             complement(36137..37768)
                     /gene="dcuS"
                     /locus_tag="ACHKYO_RS07340"
                     /old_locus_tag="pgaptmp_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGU4428336.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            37949..38179
                     /gene="yjdI"
                     /locus_tag="ACHKYO_RS07345"
                     /old_locus_tag="pgaptmp_001469"
     CDS             37949..38179
                     /gene="yjdI"
                     /locus_tag="ACHKYO_RS07345"
                     /old_locus_tag="pgaptmp_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HGU4428337.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            38191..38463
                     /gene="yjdJ"
                     /locus_tag="ACHKYO_RS07350"
                     /old_locus_tag="pgaptmp_001470"
     CDS             38191..38463
                     /gene="yjdJ"
                     /locus_tag="ACHKYO_RS07350"
                     /old_locus_tag="pgaptmp_001470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4428338.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            38690..38986
                     /gene="ghoS"
                     /locus_tag="ACHKYO_RS07355"
                     /old_locus_tag="pgaptmp_001471"
     CDS             38690..38986
                     /gene="ghoS"
                     /locus_tag="ACHKYO_RS07355"
                     /old_locus_tag="pgaptmp_001471"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU4428339.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            39014..39187
                     /gene="ghoT"
                     /locus_tag="ACHKYO_RS07360"
                     /old_locus_tag="pgaptmp_001472"
     CDS             39014..39187
                     /gene="ghoT"
                     /locus_tag="ACHKYO_RS07360"
                     /old_locus_tag="pgaptmp_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HGU4428340.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(39306..40823)
                     /gene="lysS"
                     /locus_tag="ACHKYO_RS07365"
                     /old_locus_tag="pgaptmp_001473"
     CDS             complement(39306..40823)
                     /gene="lysS"
                     /locus_tag="ACHKYO_RS07365"
                     /old_locus_tag="pgaptmp_001473"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU4428341.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(41060..42517)
                     /gene="dtpC"
                     /locus_tag="ACHKYO_RS07370"
                     /old_locus_tag="pgaptmp_001474"
     CDS             complement(41060..42517)
                     /gene="dtpC"
                     /locus_tag="ACHKYO_RS07370"
                     /old_locus_tag="pgaptmp_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HGU4428342.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(42576..44723)
                     /gene="cadA"
                     /locus_tag="ACHKYO_RS07375"
                     /old_locus_tag="pgaptmp_001475"
     CDS             complement(42576..44723)
                     /gene="cadA"
                     /locus_tag="ACHKYO_RS07375"
                     /old_locus_tag="pgaptmp_001475"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HGU4428343.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(44803..46137)
                     /gene="cadB"
                     /locus_tag="ACHKYO_RS07380"
                     /old_locus_tag="pgaptmp_001476"
     CDS             complement(44803..46137)
                     /gene="cadB"
                     /locus_tag="ACHKYO_RS07380"
                     /old_locus_tag="pgaptmp_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HGU4428344.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(46503..48041)
                     /gene="cadC"
                     /locus_tag="ACHKYO_RS07385"
                     /old_locus_tag="pgaptmp_001477"
     CDS             complement(46503..48041)
                     /gene="cadC"
                     /locus_tag="ACHKYO_RS07385"
                     /old_locus_tag="pgaptmp_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HGU4428345.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(48301..48460)
                     /locus_tag="ACHKYO_RS07390"
                     /old_locus_tag="pgaptmp_001478"
                     /pseudo
     CDS             complement(48301..48460)
                     /locus_tag="ACHKYO_RS07390"
                     /old_locus_tag="pgaptmp_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(48923..49249)
                     /locus_tag="ACHKYO_RS07395"
                     /old_locus_tag="pgaptmp_001479"
     CDS             complement(48923..49249)
                     /locus_tag="ACHKYO_RS07395"
                     /old_locus_tag="pgaptmp_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4428346.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(49246..49509)
                     /locus_tag="ACHKYO_RS07400"
                     /old_locus_tag="pgaptmp_001480"
     CDS             complement(49246..49509)
                     /locus_tag="ACHKYO_RS07400"
                     /old_locus_tag="pgaptmp_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU4428347.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            complement(49581..50447)
                     /locus_tag="ACHKYO_RS07405"
                     /old_locus_tag="pgaptmp_001481"
     CDS             complement(49581..50447)
                     /locus_tag="ACHKYO_RS07405"
                     /old_locus_tag="pgaptmp_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4428348.1"
                     /translation="MSGITISRPEVVNGHTDVICSTSIGHILAVRKTTLMQIDTLIRQ
                     LTEISAMTESIGGKTAPDWAMKQNFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISAANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHFITGQQADRLADLERMLHLFSGKPSPDNR
                     ENITLRLDDHIQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            complement(50532..50729)
                     /locus_tag="ACHKYO_RS07410"
                     /old_locus_tag="pgaptmp_001482"
     CDS             complement(50532..50729)
                     /locus_tag="ACHKYO_RS07410"
                     /old_locus_tag="pgaptmp_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4428349.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(50741..51229)
                     /locus_tag="ACHKYO_RS07415"
                     /old_locus_tag="pgaptmp_001483"
     CDS             complement(50741..51229)
                     /locus_tag="ACHKYO_RS07415"
                     /old_locus_tag="pgaptmp_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4428350.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGIELAELINKVAENGYS
                     LHVVDDSDQQSTCTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKMFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(51226..51603)
                     /locus_tag="ACHKYO_RS07420"
                     /old_locus_tag="pgaptmp_001484"
     CDS             complement(51226..51603)
                     /locus_tag="ACHKYO_RS07420"
                     /old_locus_tag="pgaptmp_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4428351.1"
                     /translation="MKTLSDTHVREASRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRMVNNITQGKHPEAQQ"
     gene            complement(51650..52024)
                     /locus_tag="ACHKYO_RS07425"
                     /old_locus_tag="pgaptmp_001485"
     CDS             complement(51650..52024)
                     /locus_tag="ACHKYO_RS07425"
                     /old_locus_tag="pgaptmp_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4428352.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVKPCFGARLVQEGNRLHYL
                     ADRAGISGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHSVTLYAKGLTCEAD
                     TLGSCGYVYLTVYPTPATATTS"
     gene            complement(52187..52408)
                     /locus_tag="ACHKYO_RS07430"
                     /old_locus_tag="pgaptmp_001486"
     CDS             complement(52187..52408)
                     /locus_tag="ACHKYO_RS07430"
                     /old_locus_tag="pgaptmp_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU4428353.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWQGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(52471..>52644)
                     /locus_tag="ACHKYO_RS07435"
                     /old_locus_tag="pgaptmp_001487"
     CDS             complement(52471..>52644)
                     /locus_tag="ACHKYO_RS07435"
                     /old_locus_tag="pgaptmp_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HGU4428354.1"
                     /translation="AVVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVG
                     GSQVFSFAEHGLL"
BASE COUNT        12537 a        13095 c        13885 g        13127 t
ORIGIN      
        1 cggcaaatac tgagcacaat ttatcaatgc aaccggtgtt tctttttaaa aaacgtaccg
       61 gagcaatggg cggtcatgtc gctggcaccg caggattctg cgttgctgtg ccagcctttt
      121 attcatggtc aatgagtgag gtgattgata aagacaccag gtcgtcagag cgcactgatt
      181 atccgcatta tgccagtgaa gttgcactgg cttttggttc acgagtggtt ggcctcgaac
      241 tggcaaaaat gattgtggat gcgtggctgg gcgcacagta cgaaggcggt cgtcatcaac
      301 aacgcgtgga ggcgattacg gcaatagagc agcggagaaa ttgagattca tccactactt
      361 gcatggatga gtaatgacta atgtggatag agtttctttt tgaggttggc taatgaaacg
      421 ctttccgcta tttcttttat tcaccctgct cacgctgtcc accgttccgg cccaggctga
      481 tattatcgac gatactattg gtaatattca gcaggcgatt aatgacgctt ataaccctga
      541 ccgtgggcga gactatgaag attcgcgcga cgacggctgg cagcgtgaag tgagcgacga
      601 tcggcgcaga caatatgacg accgccgccg ccagtttgaa gatcgccgca ggcagctgga
      661 cgatcgccag cgccagcttg atcaggagcg tcggcaactg gaggatgaag agcggagaat
      721 ggaagatgag tatgggcgat gaggttgggt atggagcagg catcgcccct caccccaatg
      781 gggagaggga gaaaacgagc gcaatattca atattgagca caatcggtcc cctcgcccct
      841 ctggggagag ggttagggtg agggggccag cgcgcacatt ccctctcatc acgccacccc
      901 aatctccatc ccatcaaacc ccacctcaaa ccctgacggt agtgcgtttt ccatcagcca
      961 cgcatcaaac tggtggctga tatgggtcag aatcacctgt gacgagcgga taacctgatt
     1021 cagcgcaagc acggtattta aatcacagtg attacgcggt gcatccgcgc gcggcgggtg
     1081 actgcaatca atcaccatca cctgcggatg attattgagt aaaaatttca gcgttttttc
     1141 cggcaggcca gcggtgtcag acagccacgc cacccggcta tgtgccgttt ccagcagata
     1201 accgaaggtc agttttgagt ggttaagcgg cagcggcgtg acctgcaacc cctgcaaatc
     1261 aaacaccaca aacggttcca ctgtgtggct gaaatcaagc aggcccggat gtttaaacag
     1321 atcgtcgcag ccctgttcat ccggcgggcc gtagaccggg atcacatcgc ccacgcccca
     1381 gcgtagcgga aatagccctt ggacgtgatc catatggtag tgcgtcagca aaaactgctg
     1441 gaacgatcca ggcgaccagc gatcggtgag atcgtgccgc ccggcgtcga tcagggtgat
     1501 cgcgtcgtta aacttcacta cgccgctgca tggctggcgg cgatactgcg gcgagcgccg
     1561 cgctctggcg caggccgcgc actcgcagcc ccatgccgga acgccctgtg cgccgccagt
     1621 gccggtgagc gtgagggtca ggctcatgtt acagcgcctt ggtgaagcgg aagtggctct
     1681 gctcgtagcc ttcacgcagg taaaaacggt gtgcgtcgtg gcgcttcacg ttggtggaaa
     1741 gctcggtcat ttcggccccg gcctggcggg cttcttcttc tgcccatgcc agtaatttac
     1801 tgccgacgtt cagaccgcgc gcctgcggca tcaccaccag ttcctgaatt tcgccgatcc
     1861 agttgacatg atgcagatga aactgcaaat gcaggccgat catgccgaca acttcgccat
     1921 ccagcagcgc cagatggtag cgcatgtttg ggtcgcgcag gttggcgtta aagcccacgc
     1981 gaaacgcatg gtggtcaaac tccgcctgtt ttagctcaca aatcagcgcg taaaccgcgt
     2041 cggtatcgta ctgcgtggcc gggcgaagct cacaagcagg catggtgttt ctccttctga
     2101 tggatcagcg tcagcagcgt gtcgaccgac tggcgcaggc tgccgtcatt gttgagcgta
     2161 tggcaatcct gtggagtata gcgggcggcg cgcgccaggc gggcgttaat ttcactggcg
     2221 ttttcacggc cacggttttc caggcgctgg cgcaggatct ccggcgaaac ctgtaaacag
     2281 acgggcagca gcgccgattg atagcgcgcc cgtgcctgcg gcaaatgcgc gcgtgaaccg
     2341 ttgacgacta cgtcgaatcc agcgtgcagc cagaggtcaa tctcgatgcc gacgccataa
     2401 tacagaccgt tagcgtgcca gcttaaggcc agcaggtttt gccccgcgcg ggtaaaaaac
     2461 tcccgctcac tcagggcgat atggttttca cttccggcgc tggcgtcgcg cgtgatgtag
     2521 cgatgcgcca ccagtaactg cgtttgttcc cgcaggcgga gttccgccag caggctgtct
     2581 ttcccggagc cggacggccc cattaaccaa atcagttttc ccatcatcag aacacccttt
     2641 taccctgacg ccagacgtgg tcgatatgaa tgtgattgcc ctggcgatgc gccagcacca
     2701 ggtcagcgcg tttaccctca ccaatcaccc cgcgatcctg aagattcagc gcctgcgccg
     2761 gatttttggt caccagcctc accgcctgcg gtagcgtaaa gcggttgctc tcgtcatcgg
     2821 cgacgcggaa cgccgcatcc agcaggctgg cggggtagta gtcggaagag aggatatcca
     2881 gcagaccaag ctgcgccagt tcactggccg ccacattgcc ggagtgcgag ccgccacgca
     2941 cgatatttgg cgcacccatc agcacgttca tgccatgctt acgcgaggct tccgctgctt
     3001 cgaacgtggt gggaaattcg gcgatcacgc tgccaagttg gtgggattcg gcaacgtggg
     3061 cgtgcgtggc gtcatcgtgg ctggcgagcg caatttgtcg cgcgcggcac agggcggcga
     3121 tggattcgcg attcggctgc gaccagcgtg cggcgagcgc caattgctct tcttcgtact
     3181 gctgcatctg cgcatcggtg agggagtatt tgccctgata atattcgcga tacttctcgc
     3241 ggttagcgaa ctggcgctgg cccggtgagt ggtccatcag cgacaccagc gtcaccggtt
     3301 cgcgctgaac cagtttttca aacagcggca gtgtggtgtg atgcggcagt tcgcagcgca
     3361 ggtgcaggcg gtgctcggcg cggttgacgc cgcgtttctg cgtctcttcg atggtgttga
     3421 tcatcttctc cagattctcc agccgatcgc cgccgtcgcg cacgtcgcca atcgccaccg
     3481 cgtccagcac ggtggtgatg ccgcttgcca ccatcagcgc gtcgtggctg ctcatcgccg
     3541 aatgggcggg ccagtcgact ttcgggcgcg gggtgaagaa tttatcgagg ttgtcggtat
     3601 gcagctcaat caggccgggt agtagccagc caccttcgcc gtccatcgct tctggcaggc
     3661 ggctctggct ttcggcaaag gcgcggattt cgccatcctg cacctccagc gaaccgtgca
     3721 ccacctcgtt ttccagcacc agcttaacgt tattgataat catgaagagg ctcccattgg
     3781 gtgcaggcgg tcggcgacgt cattacgtac agcttcgtca tggaagatgc cgacgatggc
     3841 tgcgccacgg gctttggctt cgtgaatcag ttccaccacc gcggcgctgt ttttggcgtc
     3901 cagcgaggcg gtaggttcgt cgagcagcag aatggggtag tcgacgataa agccgcgggc
     3961 gatgttgacg cgctgctgtt cgccgccgga gaaggtcgat ggtgccaggt gccacaggcg
     4021 ttccggcacg ttcaggcggg tgagaagacg cgcggcttta gcggcgcagg cttcacgcgg
     4081 aacgcctgta tcgagcagcg gctgcatcac cacttccagt gctgagatac gcgggatgac
     4141 gcgcagaaac tggctcaccc agccgatggt agttttgcgg atctccacca ctttgcgcgc
     4201 tggcgcggtg actaggtcta cccactcgtc accgtgtttg atctggattt gaccttcgtc
     4261 gggtagatag ttggcgtaca gcgagcgcag cagggttgat ttgccgctgc cggagtgacc
     4321 gtggagcacc acgcattcgc ctgcgttgac ggtgagcgag gcgcgttgta agacgggcag
     4381 gcgcacgccg ttttgctggt gcaggatgaa ggttttactg acgttttgta cgttaatcat
     4441 ttttcgcctc gtggttcgca tattggggag cggtgcgcgc attgcccctc accctaaccc
     4501 tctccccgaa ggggcgaggg gaccgattgt gcgcggtgtt gaatattgcg ccggttttct
     4561 ccctctccct ccgggagagg gccggggtga ggggatcagc gcgcacggtg ttgcattcaa
     4621 ttctgcaaaa ccgatgacac cagcagttgt gtatacggat ggtgcgggtc gtcgagcacg
     4681 cggtcggtta acccactctc caccacttgc ccctgcttca tcaccagcaa acggtccgcc
     4741 agcaggcggg cgacgcctaa atcgtgggtg acaatcacta ccgcgaggtt cagctccacc
     4801 accaggccgc gcagcaggtc gagcaggcgt gcctgtaccg acacatccag tccgccggtc
     4861 ggctcgtcca taaacaccag cttcggatgc gtcaccaggt tgcgggcgat ctgcaaacgt
     4921 tgctgcatgc cgccggaaaa ggtggtcggc aggtcgtcga tccggttggc gggaatttcc
     4981 acctcttcca gccacttctg cgcggtggca cgaatatcgc cgtaatgacg tgccccggtc
     5041 gccatcagcc gctcgccgat attgccgcct gccgacacct ggcggcgcag gccgtcgagc
     5101 ggatgctgat gcactacgcc ccattcggtg cgcagcaggc gacggcggtc ggcctcgctc
     5161 attgcgtaca gcgaacggct ctcatagtga atctccccct gctgcggcgt caggcgcgcg
     5221 gagattgatt tcagcagcgt ggtcttcccg gagccggact ccccgacaat gcccagcact
     5281 tcccccggcc ataaatcaaa agagacatcg ctaaagcctt tgcccggcgc gtaaaggtgg
     5341 gtcaggttat tgaccgaaag taacggttga ttcattggtt ttttgcctcg ctctgttggc
     5401 ggcaataatc ggtatcggag cagacaaaca tgcggtttcc ggcgtcatcc agcaccactt
     5461 catcaagata gctgtgggtc gatccgcaga tggcgcatgg ctcatcccac tgctgaacgg
     5521 tgaacgggtg atcgtcgaaa tcgagacttt ccacgcgggt aaacggcggc accgcataga
     5581 tgcgcttctc gcgccccgcg ccgaacagtt gcagggcggg catcatgtcc atttttgggt
     5641 tatcgaattt cgggatcggc gacgggtcca ttacgtagcg cccgttcacc ttcaccggat
     5701 aggcgtaggt agtggcgata tgaccgaagc gggcgatatc ttcatacagt ttcacctgca
     5761 tcacgccgta ctcttccagc gcgtgcatgg tgcgggtttc cgtttcgcgc ggctcgataa
     5821 agcgcagcgg ttcggggatc ggcacctgga agataatgat ctgatcttcc gtcagcggcg
     5881 tttcgggaat gcggtggcgc gtctggataa gcgtcgcatc atccgtacgt tcagtggtgt
     5941 tcaccccggt cacgcgctta aagaagttgc gaatcgacac ggcgttggtg gtgtcatccg
     6001 cgccctggtc aatcaccttc aacacgtcgc tttcgccaat cacgctggcg gtgagctgta
     6061 tgccgccggt tccccagccg tatggcatgg gcatctcgcg cccgccaaac ggcacctgat
     6121 aaccggggat cgccaccgct tttaagatgg cgcggcggat catgcgtttg gtctgctcgt
     6181 cgaggtaggc aaagttgtag ccgctcagat tagccatggt tctgctcctg ttgcagacgt
     6241 ttgagtagct ccagttcggc ctggaaatcg acgtagtgcg ggagtttgag gtgcgagaca
     6301 aagcctgcgg cttcgacgtt gtcggcatgc gccagcacga actcttcatc ctgcgccggg
     6361 cctgtcgcgt gctcgccgta ctccggagcc tgcaacgcac ggtcaaccag cgccatcgcc
     6421 atcgctttgc gctcgctcat gccgaacacc agcccgtagc cgcgcgtgaa gtgcggcggc
     6481 tcatccggcg ggtcaataaa accgttgacc atttcacact cggtcatcag cagttcgccg
     6541 acgttcaccg caaatcccag ctcttccggc acaatcgaca cgtcgatgta accactgcgg
     6601 atttcgcccg cgaacgggtg attgcgtccg taaccgcgct gggtagagta ggccagcgcc
     6661 agcaaatagc cttcgtcgcc gcgcatcaac tgctgcaaac gggaagaacg tgaacagggg
     6721 taaaccggcg gcgtgcgggt gatgtcatcc ggctgtgcgc cgctatcctc ttcaaacttc
     6781 gccagcccct gacgcgccag caggctgaaa acgtgcggcg acggggattg ttcgctgtct
     6841 gcggtggtca gcgtcggcgc ttcgccgttt gccagcaggg tgaaatcgag caggcgatgg
     6901 gtgtagtcgt aggttgggcc aagcaactga ccgccgggaa tatctttata aaccgctgaa
     6961 atacgccgtt cgagacgcat ctccgtggtg tcgagcggct cgcttaccgc cagcttcgcc
     7021 agcgtggtgc ggtaggcgcg tagcaggaaa atcgcttcga cgttatcgcc gctggcctgt
     7081 ttcagcgcca gcgccgccag ttcgcggtcg gcaatgccgc cttcggtcat cacgcgatct
     7141 accgcgaggt taagctgctg ttcaatctgg gcgacgctca gttcgggcaa atcggtatcg
     7201 ccccggcgtc ggctctcttg cagggcgtgg gcggcgtcga tcgccttttc gccccctttc
     7261 acggcaacgt acatcagcac acctccacat gagtggttcg cggaacagcc agcaggcgct
     7321 cgccgcaggt caggatcagg tcgatgccga gcgggaacgg gtgcgggcgc tcggtgagtt
     7381 cgtgcagaat gcactccggc agctgcggag cgatcattcg ttcttcggca atgcccgcgc
     7441 cggtgagacg caacatgcgt ccgccgctca ggctggcgac ctgtaaaatc agcgtcgcgc
     7501 ccgcttccgg cgcaacggcg gtgccggtgg aaagggcgtt gagctgttcg ctggaaatcg
     7561 cctcatccgt caccgcgaag gtcgcctgtt ccggctggct gaccagcggc gcgttggtat
     7621 gaaaacgcag gctctgattg acgatatcgt tacttaatgg cgtggcaagc cacaccggcg
     7681 tgtcgttatc ggccagcgtc agcagcacgc tggtggtggc gatattcagc ggctgccagc
     7741 cgcgtttgag ctgatgcagg gcgacaatca cgcccggctc gctcatcgcc tttaacaggc
     7801 gacgaaaact gtgctgggca tcctgcacgg gaagcataaa agcggtttcc agggtcatgc
     7861 gttgtctccg cgaaccatcg taaagaagtc gacccggctg gcgttcactt cggcctggcg
     7921 tgcggcaatg cgtgccatac ggtcagcgtc cagcggggca ataacggttt ctgataagtt
     7981 ttgaaaatag cgagattgct gcatcagcgc gtcaatcagc gcgcagcgtt cggcgtgctg
     8041 tttgtcgcgc cccagcaccc agctgtagcc gagcgtgccg tcagtcaggc gtacggcggc
     8101 gcgggtgagc gtggcgtcgc cggcaaaaaa gcgttcgccg gtgccgccca tccgcgcctg
     8161 aatctgcacc aggccggttt cggcggcgcg gataacctca tagtcggcgg tgatgttcag
     8221 tgctttcagg cgggctgcca gttcagccgg ttggctgtgc gccagtacgg acatccagtg
     8281 ctggcgggtc gcggtatctg cgtgcattca gtgctccata gtgaattcaa tcatgtcggc
     8341 gcgcgtcagg ctgacggagt actccgccgg gctgctttca ccgtcacggt ggttaagggt
     8401 gcgcacgcac agcagcggcg acatattcgg aatttcaaga cgctggcact ctttggcctg
     8461 ggcgcgacgg gcgctgatcc gcgtctggct gcggcgcagc gcaattccgg tttgctcgcg
     8521 cagaaaatcg tgcagcgaac cgctgtcgaa gcgttgcagc gtcggccaaa gggtgaggtc
     8581 cgcaaagtag tggtcgatta aacagagcgc gacgccgttg acgcgacgca gggtgcgcaa
     8641 atggataacg ttctcgccct cggtaatccc cagcgcgtca gcgacgtgac cggacgcggg
     8701 gcgcaatacc gacagcagtt tttcgctggt gggatggcta ccctggtcca gcaggttctg
     8761 gctaaaacgc gcctgggcgt tgagcgggta atcgaacggg cgcatcagca ccagcacgcc
     8821 gacgccctga cggcgctgta cccagccttt ttccaccagt tggtcgatgg cgcggcgcag
     8881 ggtgtggcgg ttcacctcaa agcgcgccgc cagttgctgc tcggcgggaa gatagtcgcc
     8941 gcagcggtag tgttgacgaa gctcctgctc aagttttgcg gctatctcct gatagcgtgt
     9001 tgggtagctg gtcggatgtg tagacaagtg catagatatc aatgcctcgc ttatcagata
     9061 aagtgcttac gcaaccgttg agagaggaaa tccagcaggc tgaccgtgac gatgataagc
     9121 accatcaggg cgcaggtttg ttggaactgg aaaccgcgaa tcgcttccca cagggtgacg
     9181 ccgatcccgc ctgcaccgac catgccgacg acggtcgccg agcggacgtt ggattcgaag
     9241 cgatagaggg agtaggagat cagcagcggc atcacctgcg gcagcacgcc gtagaggatc
     9301 tcttcgagct tgttggcgcc ggtggcgcga atgccttcca ccgggccggg ttcaatcgct
     9361 tccaccgctt cggaaagcag tttggagagc acgccggtgg tgtggataaa cagcgccagc
     9421 acgccagcga acgggccgag gccgacggcg accacgaaca gcatggcgaa gaccatttcg
     9481 ttaatggcgc ggcaggcgtc catcaggcgg cgaacgggct ggtaaaccca ccacggcacc
     9541 aggttttcgg cgctcatcag gccgaagggg atggagagaa ccaccgccag cgccgtgccc
     9601 cagacggcga tttgcagcgt gacggccatt tcggtgaggt aatcctgcca ctggctgaaa
     9661 tcgggcggga agaagtcggc ggcgaacgtc gccatgttgc cgccgtcttt aatcagcgtg
     9721 agcggggcca tttccgcgcc ctgccacgag acgaccaaca cggcgagaac aacggcccag
     9781 ctcagaagcg agaaccagct acgcttgggt ggggcgatgg tgatggtttg catgtttggc
     9841 tccggtctgt aggccggata aggtgcttgc accgcatccg gcatcaacgc ctgcacattg
     9901 tcggatgcga cgccggggcg tcttatccga cctacgattt actgcaccgc tttactcacc
     9961 gaactcatcg cgcttaacgc gttgttcagg cggtccaggt catccagctg cgcctgaatc
    10021 tcggtggttt ttgccagctt gtcctgctca ttcagtcctt tattgctttt cacgccctgc
    10081 atctctttaa acagcgcgag ctggcgaatc ggcaccagtt gcaggtcgct ggaagcgcgg
    10141 aacggcgccc agcccaggcg ttccagcacc gctttttctt ccggcgtttt gccgtagttc
    10201 ataaagaagt cgtagatctt gtctttggtg gtttcggaaa ggtttttacg ccagacgatc
    10261 ggatcgcctg ggatcagcgg cgacttccag atcaccttca gttctttcag cttctctggc
    10321 gcggaggttt tcagcttgtc gaggttttcg gtgttgttgg tggcaacatc cacctgcttg
    10381 ttggcgacgg ccagcgcgtt ggtttcatgc ccagcgttga cggtgcgctt aaagtcgctg
    10441 gcggagatat tgtttttggc aaagacgtag taaccgggga cgaggaagcc agaggtggag
    10501 ttaggatcgc cattgccgaa ggtgagatct ttccgcttcg ccagcagatc gttcaggttg
    10561 ttgatcggac tatctttgtt gacgatcaac acgctccagt aacctggcga tccatccgcc
    10621 gcgaccgtct gggcgaagac ctggccgttg gcgcgatcca ccgcttccat tgccgacagg
    10681 ttgccgtacc aggcgatatc cactttattg aagcgcattc cctggataat gcctgcgtag
    10741 tctggggcaa agaaggcgtt caccttcacg cccagcttct tctccatatc ctgtaagaat
    10801 ggcgtccatt gcggtttcag gttttgctgt gattccgttg aaataatgcc gaaattcagc
    10861 gccttttcct gctcttcggc gtgtgccggg cttaacaggg tgctgaggct gaacatgctg
    10921 gtgaaggcca gcgaggcaat tatcttagcg ttcattcgtt ttcctcaatg atggggatgt
    10981 caggcagctt tcgcgttctc ttcgatgcga ttaatgctgc ggtagagatg gtcaaaacgt
    11041 tcgttatcaa actgttggct gctgccgtcg tagaaaacgt gcccctggcg cagggcgacg
    11101 atgcgttcgc agtagcgcag ggcgtaatcc acctgatgca gcgtgacgac cacggtgatg
    11161 ccgtcgttct gattgatgtc gcgcagggtg tccatcacga tgcgggcgga ttccgggtcc
    11221 agcgaggcga tgggttcatc ggccagaatc accttcgcct gctgcatcag cgcgcgggca
    11281 atcgccacac gctgctgctg tccgccggag agggtggaaa cgcgctgatg ggcaaaatgc
    11341 accatgccaa cgcgggtcag cgcctgtaac gcgcgctgtt tctgctcgcc ggtgaaccag
    11401 ctaaaacagg tgcgccagaa cggcgtgctg ccgagcgcgc caatcagcac gttctccagt
    11461 acgctcaggc ggttcaccag gttgaattgt tggaagatgt agccggtgtt ggcgcggctt
    11521 ttgcggatat cgcgcgccag acggccttcg cgctggactg tgcggcccag cagctcgata
    11581 tggctgccgg cggatttatc gccggtaatc aaaccgctta agtgacgtaa aagggtggat
    11641 ttgccggaac ccgacggccc aagcagagcc accatttcac cgtgatgaat gttcagatca
    11701 accgcatgca gcgcctgatg ctgattgaag gttttggcga gcttctcgac acggataatc
    11761 gtttgcatga tgcagcctcc ctaaaaaagt ggctccatcg tggcggatta ttgtgacgat
    11821 ttggttaatt aaaagtgact aacagatgaa gagttaacgg gaattcgatg acagtgcggg
    11881 agggcgggcc tcccggcggg ttattgcgtt ggttgttgtt tgacgacatt aatcatccac
    11941 ggtacgccaa atttatcggt gactttgcca aagccatgcg cccagaaagt ttcctgccag
    12001 gccatttcga tttttccgtt agcggcaaga ttgtcaaacc agcgttttcc ttcttcgacc
    12061 tgttgcgaat cgagcaccag cgtaaagccg gagtagctgg cttttcctga cggtatagca
    12121 tcgctcatca tgatgtcgct tccggcaatg cgcacgttgg cgtgggcgat ggcggtatcg
    12181 ggaaattgca ttccggaagg gcagttctcg gcgctgtcct gcgctgattt tggcatttcg
    12241 ccgaagctga ttttatagag cagttcagcg cccaacgtac gttgataata agcaattgcg
    12301 tcggcacagt taccggcaaa agagaggtag ggacttaacg gcatgatggt gacctcagtt
    12361 aagagaagcc agttaagtgt agttcgaaat ttatacagat gagagagaag cctgataaga
    12421 cgcggtcagc gtcgcatcag gcagtcggca tcgttgccgg atgcggcgcg agcgccttat
    12481 ccggcttacc gatcacaata caatcagttc tttttcacaa actcagattt cagtttcatc
    12541 ggaccaaaac cgtcgatttt gcaatcgatg ttatggtcgc cttcaaccag gcggatgttt
    12601 ttcactttgg tgccaatttt cagcatcgaa gagctacctt tcaccttcag atctttaatg
    12661 atggtaacgc tgtcgccgtc agccagcaga ttgccgttag catctttaac gatcagctcg
    12721 tcgctttcct gtgcaggttc tgcgtcgttc cattcgtagg cacattccgg gcagatgtac
    12781 atgccgttat cttcgtaagt gtattcggag ttgcattttg ggcagtgtgg taatgacatg
    12841 tggatttcct caaaagtcag cagggcaaaa gcgccaaaaa acggcagatt gccgaaaaag
    12901 gccgcaatta tacacaaaat ccctgagttt gtcgggggta ctgctgattt tagacgacga
    12961 tcgttttcat ttgaaaggaa acgcaaaaag aaaataatct ttttgcctct atttattatt
    13021 taagataatg ttaatgctct acttgttctt ttttttattc acatgatatt tttatctttc
    13081 tgacgtttta aacgtatcag aagacgttat ttattctgat aaaaaacaac acactaaata
    13141 taaaattatt tcttgcagta attataaaag ccgaaaacag aactcaaaaa caatctggct
    13201 aaataaaagt aacaaaattt gctttaagga agaattttct atgtacacac agaccctgta
    13261 tgagttaagt caggaggctg aacgcctgtt acagctttct cgccaacagt tgcagttact
    13321 ggaaaaaatg cctctctctg tacccggaga cgacgcgcca caactggctt taccctggag
    13381 tcagcctaat atcgccgaac gtcacgcgat gctgaataat gagttgcgta aaatttcccg
    13441 actggaaatg gtgctggcaa ttgtcggtac catgaaagca gggaaatcaa ccaccattaa
    13501 tgccattgtt ggtacggagg ttctgcctaa tcgtaatcgc ccaatgactg cgctgccgac
    13561 gcttattcgc catacgcccg ggcaaaagga accggtactg catttttcac atgtcgcgcc
    13621 aatcgattgt ttaattcaac aattacaaca gcgcctgcgt gattgcgata ttaagcatct
    13681 gaccgatgtg ctggaaatag ataaagatat gcgtgcgctt atgcagcgga tcgaaaatgg
    13741 cgtcgctttc gaaaaatatt atctgggtgc ccagcctatt tttcattgtc tgaaaagttt
    13801 gaatgattta gtgcgactgg cgaaggcgct ggacgtcgat tttccttttt ctgcttacgc
    13861 cgccattgag catattcccg tgattgaagt ggagtttgtc catctggcgg ggctggagag
    13921 ttatcccggt cagttgacgt tactggatac ccccgggcca aatgaagccg ggcaaccgca
    13981 tctgcaaaaa atgcttaacc agcagctggc acgcgcctcg gcggtactgg cggtgctgga
    14041 ttatacgcaa ctgaaatcga tctccgatga agaggtccgt gaggcgattt tggcggtggg
    14101 gcaatcggtg ccgctgtatg tgctggtcaa taagttcgat caacaggatc gtaacagtga
    14161 cgacgccgac caggtgcggg cactgatttc cgggacgctg atgaaaggct gtattacgcc
    14221 acagcagata tttccggtgt cgtcgatgtg gggctacctg gcgaatcggg cgcgccatga
    14281 gttagccaac aacggtaagt taccagcgcc agagcaacaa cgctgggtgg aagattttgc
    14341 ccatgccgcg ctcggcaggc gctggcgtca tgccgatctg gcggacctcg aacatattcg
    14401 tcatgctgcc gatcagttgt gggaagattc gctgttcgcc cagccaattc aggcgttgct
    14461 tcatgccgct tacgctaacg cctcgttgta tgctttgcga tctgctgcgc ataaactgtt
    14521 gaattacgcg cagcaggcgc gggaatacct ggattttcgt gcgcacgggt taaacgtcgc
    14581 ttgtgaacaa ttgcggcaaa atatccatca ggtcgaagaa agtttgcagc tattgcaact
    14641 caatcaggcg caggtgagcg gcgagattaa acatgaaatc gagctggccc tgacctccgc
    14701 caaccacttt ctgcgtcaac aacaagatgc gctgaatgcg cagttagccg ccttgtttca
    14761 ggatgattcg gagccgttaa gcgagatgcg tacccgctgt gagacactgt tacagacggc
    14821 gcagaacacc atcagtcgcg actttacgct gcgttttgcc gagctggaat ccaccctttg
    14881 ccgggtgtta accgatgtta ttcgccccat tgagcaacaa gtcaaaatgg aattgagcga
    14941 gtcagggttt cgtcctgggt ttcattttcc tgtttttcac ggcgtagttc cccacttcaa
    15001 cactcgccag ctgttcagtg cagtcatttc gcgccaggat gcaacggacg agcagagcac
    15061 gcgtttaggc gttgtgcgtg agactttttc gcgctggttg aatcagcccg actggggacg
    15121 gggaaatgag aaatccccga cagaaacggt tgattacagt gtgttgcaac gagcattaag
    15181 cgcagaagtc gatctttatt gccaacaaat ggctaaagtt ctggcagagc aggtcgatga
    15241 atctgttacg gcaggcatga atactttttt cgctgagttc gcttcatgtt tgacggaatt
    15301 acagacgcgt ttacgcgaaa gcctggctct gcgtcaacaa aatgaatcgg tggtcaggct
    15361 gatgcagcag caattgcagc agactgtgat gactcacggc tggatttaca ccgacgccca
    15421 gctgttacgc gatgatattc aaacactttt cacggcagaa cgatattgac caagacgtta
    15481 ctggacggcc ccggtcgcgt gctggagtcg gtttatcccc gctttttagt ggatctggcg
    15541 cagggcgatg atgcccgcct tccacaagcc catcagcagc agtttcgtga acgactgatg
    15601 caggaacttc tttcgcgtgt gcagcttcag acatggacga acggcggcat gttaaatgcg
    15661 ccgcttagcc tgcgactgac aatggtggaa aaactggcgt cgatgctgga tcccggtcat
    15721 ctggcgctga cgcagatcgc gcagcatctg gcgctgctgc aaaaaatgga tcaccgccag
    15781 cactctgctt tcccggagct cccccagcaa attgccgcct tgtatgagtg gttttcagcc
    15841 cgttgtcgct ggaaggaaaa ggcattaacg caacgaggcc tactggtgca ggcgggtgaa
    15901 cagagcgagc aaatttttac ccgctggcgt gctggggcgt ataacgccgg tcgttgcctg
    15961 ggcgctgttt tatcgttctc gaagagttgc gttggggggt atttggcgat gcctgtcgtc
    16021 tgggaagccc gcaagcggtg gcgttgttgc tgggtgattt gcgcgtgaaa gcgacacaac
    16081 atctggcaga gagtatcaat gcggccccga ccacgcgtca ctattaccat cagtggtttg
    16141 cctcttcgac cgttccgacg ggcggggatc atgctgattt tttaagttgg ctgggaaagt
    16201 ggaccacggc agataaacaa cccgtttgct ggtcagtgac ccaacgctgg caaactgtcg
    16261 cgctggggat gccacgactc tgttcagcgc agcgtctggc gggggcaatg gtcgaggaaa
    16321 tcttctctgt aaatttggcg taaataatca gttacatcaa tgagtcctaa acgaaatcca
    16381 tgtgtgaagt tgatcacaaa tttaaacact ggtagggtaa aaaggtcatt aactgcccaa
    16441 ttcaggcgtc aactggtttg attgtacatt ccttaaccgg agggtgtaag caaacccgct
    16501 acgcttgtta cagagattgc atcctgcaat tcccgctccc cttttgcggc cgtcgcgctg
    16561 atttttctgg cgtttgcgga aatgggccaa ctctgcgagg aaagttatgc tgaaaaggaa
    16621 aaaagtaaaa ccgattaccc ttcgtgatgt caccattatt gatgacggta aactgcgtaa
    16681 agccattacc gcagcatcac tgggtaatgc aatggaatgg tttgattttg gtgtttatgg
    16741 ttttgttgct tacgcattag gtaaagtttt tttcccgggg gctgacccca gcgtgcagat
    16801 ggttgctgca cttgccacgt tctccgttcc ctttctgatt cgaccgcttg gcgggctctt
    16861 ctttggtatg ttgggcgata aatatggtcg ccagaagatc ctcgctatca ctattgtgat
    16921 tatgtcgatc agtacgttct gtattggctt aataccgtcc tacgacacga ttggtatttg
    16981 ggcaccgatt ctgctgttga tctgtaagat ggcacaaggt ttctcggtcg gcggtgaata
    17041 taccggggcg tcgatatttg ttgcggaata ctcacctgac cgtaaacgtg gctttatggg
    17101 cagctggctg gacttcggtt ctattgccgg gtttgtgctg ggtgctggcg tagtggtgtt
    17161 aatttcgacc attgtcggcg aagcgaactt cctcgactgg ggctggcgta ttccgttctt
    17221 tattgctctg ccgttaggga ttatcgggct ttacctgcgc catgcgctgg aagaaactcc
    17281 ggcgttccag cagcatgtcg ataaactgga acagggcgac cgcgaaggtt tgcaggatgg
    17341 cccgaaagtc tcgtttaaag agattgccac caaacactgg cgcagcctgt tgacatgtat
    17401 tggtctggta attgccacca acgtgactta ctacatgttg ctgacctata tgccgagtta
    17461 tttgtcgcat aacctgcatt actccgaaga ccacggggtg ctgattatta tcgccattat
    17521 gatcggtatg ctgtttgtcc agccggtgat gggcttgctg agtgaccgtt ttggccgtcg
    17581 tccgtttgtg ctacttggta gtgttgccct gtttgtgttg gcgatcccgg cgtttattct
    17641 gattaacagt aacgtcatcg gcctgatttt tgccgggtta ctgatgctgg cggtgatcct
    17701 taactgcttt acgggcgtta tggcttctac cttgccagcg atgttcccga cgcatatccg
    17761 ttacagcgcg ctggcggcgg catttaatat ttcggtgctg gttgccggtc tgacgccaac
    17821 actggcggcc tggctggtcg aaagctcgca gaatctgatg atgcctgcct attacctgat
    17881 ggtagtggcg gtgattggtt taatcaccgg cgtaaccatg aaagagacgg caaatcgtcc
    17941 gttgaaaggt gcgacaccgg cggcgtcaga tatacaggaa gcgaaggaaa ttctcgtcga
    18001 gcattacgat aatatcgagc agaaaatcga taatattgac cacgagattg ccgatttgca
    18061 ggcgaaacgt acccgcctgg tgcagcaaca tccgcgaatt gatgaataag ctgaaacgga
    18121 tggcctgatg tgacgctgtc ttatcaggcc aattgaactc ttaaggttca cttaatctct
    18181 gacgcgcata ctctcctcca ggttaacgga ggagagtgca atgaaaaacc gtgtttatga
    18241 aagtttaact accgtgttca gcgtgctggt ggtcagcagc tttctttata tctggtttgc
    18301 cacgttctga tctttcttca gccgtaccca ggcccgcgtg ccggaagtct cttgccggtt
    18361 ttgcaggaaa aactgcccgt gatgcaactg tgtaatgcgg ctgacaatac ttaaccccag
    18421 accaatcccg ccataacggc tgtccatacg tacaaacgct ttactcaact ccccgcattt
    18481 actctcatca atacctggtc cttcatcttc aactgccatg accgctccgc catcttcttg
    18541 cagcttaatc ataatgttgc tgccttgcgg gctgtaacga tgggcgtttt ctaccaggtt
    18601 tcgcaataac atccgcagca gggttgcatc accctgaacg gtgatgtcgg cggcgctctc
    18661 tggcaatagc agggtttgct gtcgctggtc gagcatggta ctgagttcgt catacgaggg
    18721 gagaatgaca tcttccagca gttttacatg ttgataatta ccggaagaaa atgactgtcc
    18781 ggcacgcgcc agttgcagca gctgggagac gctctccatc atctgatcaa gccgtgccac
    18841 taacggtgct acatcaatgt gatgcgtttt cgccagcagt tccagatgca aacgcacccc
    18901 cgccagtggc gttcgcagtt cgtgcgcgac gtcagcggta aacaaccttt cgttatccag
    18961 cgtgctggtc aggcgactga ccagatcgtt caacgccgaa accaccgctt cgatttcgag
    19021 ggtggcgctg tgaatggcaa tgggcgttaa gttgtcggcg gtgcgcgctt ccagctcttt
    19081 ttgcagctcc gccagcgggc gagtgatgcg gcgtaccgcc tgatagcaga taaatagcgt
    19141 caggctgacc ataaagacgc cggggacaat caggctggcg accgcctcgc ggatctcacg
    19201 catgatgtgg cgatcgttgt tgcgattgtc gcgtagcgcc tgctcaaaca gctgaatctg
    19261 ctcggtactt tcatgccata gccagaagac gctgatcagc tcaaacacca acaaaatggc
    19321 cccgatggtc aatatcagcc gttggcgcag cgatattggt cggcgcagaa aatgcatcag
    19381 attcaattag ttttcctcat tcgcgaccag catatagcca aagccgcgca cggtgcggat
    19441 acgggctttg cccactttgt cgcgcagatt gtggatatgc acttccaggg tgttggtcga
    19501 gggttcattg tcccagttat agatgtcgtt gtagagaatt tcccgatgca ccggactgcc
    19561 tgctttgagc attaaccgtg acagcagagc atattctttg ggcgtcagaa tcaactcttc
    19621 accgcccatc catacctgac ggcgacccat attcagcgtc agattgccaa caatcagctc
    19681 actttcgccc tgattattat ggcgtcgtag cagggcgcgg atacgggcgt gtaactcttc
    19741 cagcgcaaaa ggcttcacca gatagtcatc ggcaccgaca tccagcccag cgattttgtc
    19801 ggtcagcgta tcgcgagcgg tgaggatcag tactggcagg gtgtattttt tctgccggat
    19861 acgggcgaga aaatgcagtc catcttcgtc gggtaaccct aaatccagta ccaccaggct
    19921 gtaatgaccg gcctcaaggc tttgttccgc catccgcgcg gttgtcacgc catcgcacgc
    19981 gtagccttca gtttgcgccg ccagaatcaa tccctgcaat aacagcgtat cgtcttcaac
    20041 aatcagaatt ttcattcact cactctcctg caagtttgca gaatatcatc cgcagcctgg
    20101 taatacttcg tctcaacgcc agttaatccc aatagcgtgg agaataaatt gtcttgtgaa
    20161 tagtgttgcg tttgcgcctg tttttgcagg cagttctggt caacctgata ccgtttttga
    20221 taatcctccg acagccacag cagcatcggc acctgttttt ggctatccgg ggcgatggca
    20281 taaggcagac cgtgcagata gatgccattt tcacctaacg attcaccgtg gtcagaaaga
    20341 taaaccaggc tggtggtaaa tttatcctga tgttctttca gcagattaat cgctttatca
    20401 acaatatagt cgacgtaaac cagcgtgttg tcgtaagtgt tcaccagttg ctctttgcta
    20461 caggtctgga tctcattggt gtcgcaggtt ggggtaaatt tcctgaactg cggcggatag
    20521 cggttgtaat aggtcggacc gtggctgccg atggtgtgta agacaatcac gccatcaccc
    20581 tgcaggttat tgatgtactc ttccagcccg tggaacagca cttcgtcata gcattcgccg
    20641 ttgatgcact gaccaggcag attcagcgcg gtgacgttct ggtgaggcac gcggtcgcag
    20701 gcacctttac agccgccatc gttgtcattc cacagcacgt tgatgcccgc tcgctgaatg
    20761 atatccagca cgccttcctg gtgctgtgcc agctcttctt tgtaatgctc acgcggcata
    20821 tccgagaaca tgcacggtac tgaaactgcc gttgccgtgc cgcaagatgc ggtattaggg
    20881 aaatagacca cgttatcttt cgccagccgc gggttagttt cacgcgggta gccgttgagg
    20941 gagaagttct ccgcccgcga ggtttcgccg acaatcagga tggtcaaatt tttacgtttt
    21001 tcgttctgca ttaacgggtt gcggtgggcg tcttcaccaa ttcgcaccag cggtagattt
    21061 gccagtcgct gatgggagta ccatgaccag ctggcaacaa tgctgttaga ggggcttaag
    21121 gatttcacca gctctttgtt attgcggaac aacgaggcgt agtctttata aaacagtgcg
    21181 gcgaccagca aaatcagtag tacagaaacc agaatattgg ctccacggaa aagaacactg
    21241 cgcagacgcg aggtggcagg tttgattttt atccagcagg caatcagcgc agcaagcacg
    21301 ccgctgaatc ccagcgttaa taacatttgc ggtgtcatca gcgcataact ttctgccgga
    21361 gtggtatcaa taatattggc aatcatcgag cggtcgatga cgatgccgta agtcattatg
    21421 aaatattgtg cagccgcgcc aaccagaata aacaggcagg ccagtggtcg attaagccat
    21481 aagaaagagc ttagtgtcag gacaatatta atcacgctga aagcgacgac cggcatcgac
    21541 aagaaaacca gtacgttatg cagcgaatcc agcggcagcg cctgcaacac ctgtttaaaa
    21601 aaggcaatat tcaggcagat agagatataa aaagcggcca acaatagcca ggcgagtaaa
    21661 ttcaaagagg gtctttttag taggcgcttc aacatcacgg tgtttccatc gaacaaagtg
    21721 cgcatatgct cgcaaagcaa aattaagcca accttaagtt cttaaggttg gcttttatgt
    21781 ttgctggatt aaggggaatt aatctttgct tattggtgca tctaagggat acgggttttt
    21841 atgcagccgg ttgtaattca gcgcatacat ggcggtgatt accatcaggg tgacaaatga
    21901 ccacataacc tctttcgccc cggaccccac cacggcccaa atgcagtaga ggaaggcaat
    21961 ggtagtaaca gccagatatg ccgggcgtgc tttaccaaag tgaccgtgac cgagcagcag
    22021 taacgccgca caggtgtaaa gatatggcac cagtgtaaag atgaccgaga cggaagaaac
    22081 cagaccgaac tctttggtcg cgtttggtga aatgctgctg agctggaaga tggtcatcaa
    22141 aataccgacg ataatcagcc ccgccactgg cgtgcccgct ttatttacac gggcaaaaat
    22201 cggtgggaac agtccgtcat cggcagcggc tttcgccgtt tgacccgcca gcaacgtcca
    22261 gccgcccagt gaacctaagc aacccgcagc tgcgcagaag gaaacaatgg ccccggcggt
    22321 gtcacccaac gccatccgtg cggcatcacc gaatggcgaa gcagaaacgc gcagtgcggc
    22381 attagggatc atccccataa tcgcggtggt agaaagtaca tagcaaacgg cggcaatcaa
    22441 taccccacca atggtggcga tagggacatt gcgtttcggg tttttcacca cacctgcggc
    22501 aacggaggca ctttccacac cgatgaacga ccacagcgta acgttaaggg tactttgaat
    22561 tgcaccgaag gtgcccaggc cgctgacgtt ccatgccgcc atataggttt caccacggaa
    22621 ccagaaccag ccaaatacgg caatcccgac gatggggatc agcgccagca cggtggcaac
    22681 tgcctgcaca cgggtgatca ttttcggacc gacaatgttc agcaggacga agatccacag
    22741 caccacgacg caggtgatgg ttaataccaa tggatctttc agaatcggga agaagtaact
    22801 taaatatcct acgccaatga ccaccatggc gatattgccg atccagcagg ccagccagta
    22861 gaggacgttg gtttgataac cgagaaacgg gccaaagcag cggcgggcgt aagcgtaaga
    22921 accaccagga cttggatcga ggaacgacat tttggcgtat accatcgaga gccccagcgc
    22981 accgataatc gtcaccaacc atccataaat ggcaatcccg ccagtggagg ccaggtttgc
    23041 aggtagcaga aaaacacctg accccataat attccccgac accatcaggg tgacggggat
    23101 taagcccact ttgtgagcat cagcatccga agacataatt aaactcctgc gaaggcgagc
    23161 ttcgtgacaa taaattacgt catatcatac gcctgcattc gatagttgaa ttattatcga
    23221 ccgggttatc gctgatgccg ttattaatgt agctcttcgt taaggttaaa aatcaggcga
    23281 cagtgcgttc tctgtgctga ctaacataat gcagcggcgt cataccgtag aagtctttaa
    23341 agacagaaat aaagtacgac gtactgttgt agccgcagga ctgtgatacc tgagagatat
    23401 ttttaccgtc catcattaat tcatttacgg cataacgcat gcggcaggtg gtgattattt
    23461 ggctataact ggtgttttcg cttttcaact ttttctttaa cagacttggg ctaagacata
    23521 aacaactggc taccatacta agattccagt ctttgtgaat atcgctttca ataatttgat
    23581 aaacgctgtc acttacacaa ttacgtaaca tatacatcat taatgaaagg aattttttac
    23641 tgtcgagaaa acgggatagc acagtgaata ataatgcgcg tgttcgttcc ttttctgact
    23701 cagtttctgc gggcatcatg ctgtgttgcg cggcaacgcg aaagacgtct ggcgtcaggc
    23761 atggcagggt gagtatgggc gtagcgctac gttgccatac tggtatttgc gagagatctt
    23821 tattcagaaa ccggaggtaa tctttgatga tgtcgtgact aatatgagca accaaagtgt
    23881 tgttaagaga ggagacgtcg ataatattat tttcacaatt taataacgcc atatggttag
    23941 ctttaaggct aataggttct ttcccattca cccttatcca gacatctttt tcagtcaata
    24001 aaacaataca aggttggtcg ctgcaaatcc tcatacatgt actcctgagt gcgaataaaa
    24061 gaccagttct cgctaaagca aagcgatacc gggaaatggt tatataattg tatgatgaat
    24121 ataaacatgc ataaaaataa cgagttttac taatattaca aatttttata gtaatttctc
    24181 atgtacatat ttttttacag gataaataac aggcaaaaaa aagcgcgggg tggggccgcg
    24241 caaaaagtat gacatttttg tttgcagcaa tttttatgaa aacgttgcca tactcaaaag
    24301 gcctaaggcc aggcggcgta atgttattta aacaattacg ccttcagcgg aatagtggtt
    24361 acgctttcac gcacataacg tggtaaatac cgtcaataat ttcagtccct tcagtttcgt
    24421 gttcaaatcc agggaaatgg tggtcccagg attgcagcga gcgtaaataa cttacttgcg
    24481 gactgttttt atcgccgaag ttttcaccag acagcagcat cgggattcct ggcggatacg
    24541 ggataactga gtttgccgcg atgcgtcctg gcagattttc aatggatacc agttcgacat
    24601 tgttgtcgac aatcgcgttg tacgcttcac gcggggtgac ttccgccacc ggcaggccgg
    24661 aataggcttc gttcaaccgt gcgccagggt tgttttcttt cagccaggca aacatggtgt
    24721 cacccagatc gtgaatcccc atgttcgcgt aagtgtcagg atattgttca acaagttccg
    24781 gcatcacctg cgccagcggt gtgttggcgt catagtggcg tttgaaggag caaagggtgt
    24841 taaccagagt tccccatttc ccacgggtta cgcccataga gaacaggaac ataatttgga
    24901 agtcagtggt gcgggtaggt acaatgccgt ggcgaccaag ccaggcagtg accagcgccg
    24961 ccggaacacc ggtttcttcc agttcaccat cttcacccat tcccggagca aggatgctga
    25021 ctttaatcgg gtcgagcata ctccagttat ccggaatatc tttgaagccg tgccagcttt
    25081 cgcccggatg cattacccag cagtcctgaa cggtggtcag cagtttggtt ggtgcgtcag
    25141 caaagtcata ggttttgccg gtttgtgggt cggtgacgac ttctttgttc cacggtttga
    25201 agaaccagct accgtcagcg gtgaactctt tatacagccg cgccatcgcc tgacgaaaat
    25261 caaccgcttc gtcaatcact tcttgtgtca gtgacaggcc gctgttgccg tccatcatcg
    25321 acaccgccac gtcgttggat gcgcagatgg catacagcgg ggaggtggtg gcatgcatca
    25381 tgtaggcctg gttgaagcgg gagaagttaa tcgccccacg accttcacgt acatgaatat
    25441 aagaagcctg tgacagcgca ttcagcagtt tgtgggtgga gtgggtggcg aaaacggtag
    25501 gaccgttgtg atcgccaggt tcgccgcgca tggcatagtg atcggcatag atcgggttga
    25561 aacgtgcata gccgtaccag gcttcgtcaa agtgcagacg atcggaggtt ttttccagca
    25621 gatcctgcgc ttctttagcg ttataacaca cgccgtcata ggtgcagttg gtcaccacgc
    25681 agtaagacgg tttttgccca gctttgtctt tagtcagcgg gctttcactg attttcttct
    25741 gcaaggtttc aggttgcatt tcctgcggat agattggccc gataatgccg tagcggttgc
    25801 ggcttggcac catgtagact ggtttcgcgc ctgtcagcat caagccttgt tcgatggatt
    25861 tatggcaatt acggtcaacg accacgacat cgttatcggt catgcaagcc tgcatgatgg
    25921 tgcggttaga gccggaagta ccgacgacta ccgaccagga gcgatcggca ccaaatacgc
    25981 gtgcggcata tttttcgctt tcgccaaatg cgccagtatg gtcaagcaaa gaaccgaggg
    26041 aagttcgttc gatgcccatg tcggtgcgaa acagattttc accatagtag tcatggtaga
    26101 aacgtccggc gggtgttttg gtaaaaccaa cgccgccctg gtggcctggc gctgcccagg
    26161 aatattcatg gatgtcacta tatttcatca gcgcgctgaa cagtggcggc aacagctgct
    26221 ggcggtagcg ggtcatcgcg gcaacggcgc gtccggcgat aaagtcggcg gtatcttcca
    26281 gaatccaggc gaattcatcg acaagctcca gcagatcccg gtccattgcg gcgagggctt
    26341 tttcccgatc gcccaacagg aagaccggca cgttttgttg gcgctcatga agcttaccga
    26401 tcaattgtct gacgttttga tgttcgtccg gatgttccat ttgatagctg aacatcaggc
    26461 agtcaatggc ttcgtttgaa gagagaatgg caaaaccatc atcaaaggag gtggatttaa
    26521 tcacggtaac attttgctgg cttaaagcat ctgccagacg ctcaacggcg ttaccgaccc
    26581 aggtgtcttg atggagaaac tcgctttcaa caattaatac tttcatcatt gcttacccgg
    26641 ttatgaagga atcttcgttg cgggcaagta tcttccggcc ttttcgggct gtaaagcgcg
    26701 tgaaaaacaa acatttttga aatatttagt tttataaaca atacgttatg tgattatttt
    26761 aaattatttt cactgcttat tatcgacggc taaactattt ttttggctga tactgatatc
    26821 gtctttagcc gggaaacgtc tggcggcgct gttggctaag tttgcggtat tgttgcggcg
    26881 acatgccgac atatttgccg aacgtgctgt aaaaacgact acttgaacga aagcctgccg
    26941 tcagggcaat atcgagaata cttttatcgg tatcgctcag taacgcgcga acgtggttga
    27001 tgcgcatcgc ggtaatgtac tgtttcatcg tcaattgcat gacccgctgg aatatcccca
    27061 ttgcatagtt ggcgttaagt ttgacgtgct cagccacatc gttgatggtc agcgcctgat
    27121 catagttttc ggcaataaag cccagcatct ggctaacata aaattgcgca tggcgcgaga
    27181 cgctgttttt gtgtgtgcgc gaggttttat tgaccagaat cggttcccag ccagagaggc
    27241 taaatcgctt gagcatcagg ccaatttcat caatggcgag ctggcgaatt tgctcgttcg
    27301 gactgtttaa ttcctgctgc cagcggcgca cttcaaacgg gctaagttgc tgtgtggcca
    27361 gtgatttgat caccatgccg tgagtgacgt ggttaatcag gtctttatcc agcggccagg
    27421 agagaaacag atgcatcggc agattaaaaa tcgccatgct ctgacaggtt ccggtatctg
    27481 ttagttggtg cggtgtacag gcccagaaca gcgtgatatg accctgattg atattcactt
    27541 tttcattgtt gatcaggtat tccacatcgc catcgaaagg cacattcact tcgacctgac
    27601 catgccagtg gctggtgggc atgatatgcg gtgcgcgaaa ctcaatctcc atccgctggt
    27661 attccgaata cagcgacagc gggctgcggg tctgtttttc gtcgctgctg cacataaacg
    27721 tatctgtatt catggatggc tctctttcct ggaatatcag aattatggca ggagtgaggg
    27781 aggatgactg cgagtgggag cacggttttc accctcttcc cagagaggcg aggggagcgt
    27841 ccgagcatca tgaggccgaa aactctgctt ttcaggtaaa ttattaccat aaactcagat
    27901 ttactgctgc ttcacgcagg atctgagttt atgggaatgc tcaacctgga agccggaggt
    27961 tttctgcaga ttcgcctgcc atgatgaagt tattcaagca agccaggaga tctgcatgat
    28021 gactgcaccc aaaattacat ttatcggcgc tggttcgacg attttcgtta aaaatattct
    28081 tggtgatgtg ttccatcgcg aggcgctgaa aacggcgcat attgccctga tggacattga
    28141 tcccacccgc ctggaagagt cgcatattgt ggtgcgtaag ctgatggatt cagcaggggc
    28201 cagcggcaaa atcacctgcc acacccaaca gaaagaagcc ttagaggatg ccgattttgt
    28261 cgtggtggca tttcagattg gcggttatga accttgcacg gtgactgatt tcgaggtctg
    28321 taagcggcat ggtctggaac aaaccattgc cgatacgttg gggccgggcg gtattatgcg
    28381 cgcgctacgt accattccgc atctgtggca aatttgcgag gacatgacgg aagtctgccc
    28441 cgatgccacc atgctcaact atgttaaccc catggcgatg aatacctggg cgatgtatgc
    28501 ccgttatccg catatcaaac aggtcgggct gtgccattcg gtgcagggaa cggcggaaga
    28561 gctggcgcgt gatctcaata tcgacccagc tacgctgcgt taccgctgcg caggtatcaa
    28621 ccatatggcg ttttacctgg agctggagcg caaaaccgcc gacggcagtt acgtgaatct
    28681 ctacccggaa ctgctggcgg cttatgaagc agggcaggca ccgaagccaa atattcatgg
    28741 taatactcgc tgccagaata ttgtgcgcta tgaaatgttc aaaaagctgg gctacttcgt
    28801 cacggaatcg tcagaacatt ttgccgaata tacgccgtgg tttattaagc caggtcgtga
    28861 ggatttgatt gagcgttata aagtaccgct ggatgagtac ccgaaacgct gcgtcgagca
    28921 gctggcgaac tggcataaag agctggagga gtataaaaac gcctcccgga ttgatattaa
    28981 accgtcacgg gaatatgcca gcacaatcat gaacgctatc tggactggcg agccgagtgt
    29041 gatttacggc aacgtccgta acgatggttt gattgataac ctgccacaag gatgttgcgt
    29101 ggaagtagcc tgtctggttg atgctaatgg cattcagcca accaaagtcg gtacgctacc
    29161 ttcgcatctg gccgccctga tgcaaaccaa catcaacgta cagacgctgc tgaccgaagc
    29221 tattcttacg gaaaatcgcg accgtgttta ccacgccgcg atgatggacc cgcatactgc
    29281 cgccgtgctg ggcattgacg aaatatatgc tcttgttgac gacctgattg ccgcccacgg
    29341 cgactggctg ccaggctggt tgcaccgtta aaacgcgact aaacgctact gcgccggggg
    29401 atttattccg gcgcacacct ctgacgatac caataacaga aggcgggcgt tggtaacagc
    29461 gacccgatac cctatgagca tttcaatgac tacaaaactc agttatggat ttggagcgtt
    29521 cgggaaggat tttgcgatcg gcattgtgta tatgtacctc atgtattact acaccgatgt
    29581 cgtcgggctg tctgtgggtt tggtcggtac tttgtttctg gtggcgagga tctgggatgc
    29641 tattaacgat ccgattatgg gatggattgt aaatgctacg cgatcgcgat ggggtaagtt
    29701 caaaccctgg atcctgatcg gtacgttggc aaactctgta atcttatttc tcctctttag
    29761 tgcgcatctg tttgaaggta ctactcagat tgtctttgtt tgcgtgacct acatcctctg
    29821 gggcatgact tacaccatta tggatattcc cttctggtcg ctggttccaa ccatcacgct
    29881 cgataaacgt gagcgcgaac aactggttcc ttatccgcgt ttttttgcca gtctggcagg
    29941 ctttgttacg gcaggtgtga cgctaccatt tgttaattat gtcggcggtg gcgatcgggg
    30001 atttggcttt cagatgttca ctctggtact gatcgccttt tttattgttt caaccatcat
    30061 cactctgcgc aatgtgcatg aagtcttttc gtcagacaat caaccgtctg ctgaaggaag
    30121 ccatctgaca cttaaagcca tcgttgcgct aatttataaa aacgatcagc tttcatgcct
    30181 cttgggtatg gctcttgctt ataatgtagc cagcaacatt attaccggat ttgctatcta
    30241 ttatttctca tatgttatcg gtgatgcgga tttgttcccc tattatctgt cgtatgcggg
    30301 agctgctaac ctggtgacgt tagtattctt cccacgctta gttaaatcat tatcccgacg
    30361 cattttatgg gccggagcat ctattcttcc ggtgttaagc tgtggtgttc tcctgttaat
    30421 ggcattaatg agctatcaca acgtcgtcct cattgtgatt gcgggtattt tgctgaatgt
    30481 gggaacggcg cttttctggg tattacaggt catcatggtg gcagataccg ttgattacgg
    30541 tgaatataaa ctgcacgtac gctgtgaaag tatcgcttac tccgtgcaga ctatggtggt
    30601 gaagggcggt tcagcctttg cggctttttt cattgcggtt gtgttaggga tgattggcta
    30661 tgtaccgaat gttgaacagt ctacgcaagc cctattaggt atgcagttta ttatgattgc
    30721 tctaccaact ctgtttttca tggtaacgct gattctctac ttccgtttct atcgcctcaa
    30781 tggcgacacg ctgcgcagga tccagattca tctgctggat aaatatcgca aagtaccgcc
    30841 cgagcctgtt catgctgata ttccggtcgg tgcagtgagt gatgtgaaag cctgacgtgg
    30901 acaatagcta acgaaaatgg cctgatgcga tatgtttatc aggccacgtc ttaatgtaat
    30961 gctttggatt tgcaaatttt gtagaccgga taaggaattc acgccgcatc cggcatcaac
    31021 aaagcgcacg ttgttatccg gttatcaagc caaagcgccg tagctggcgg caatgaaagc
    31081 gagcgaggaa tatcaccgtc gttaatgcgc caagcagcgc gcagaacata tcggattgcg
    31141 tgtcccactg gtcgccctgg gtgccgagaa aatcatccgc tccctggccc attgccagcg
    31201 ccgcccacca ctcgattaat tcatacatcg cgcttatcgc cagcgccacg cagcagacca
    31261 ggaacgccac cattttacgt ccgcgcacgt acatcccgcg aacgagaatt tctcgtgcca
    31321 ccagtgcagg caccagcccc tggaaaaaat gccccagctt gtcatacgga ttacggctca
    31381 accctaacca ttcctgcacc tcaaaaccaa cggggacttt cgcgtaggtg tattgtccgc
    31441 cgaccatcag gatgatggcg tgaaagaaaa tgagcgtata gagcagcggc gttaacggat
    31501 aacgtttcgc ggtggcaagg agtagcggca cgacaataat caccggtgtc acttccatca
    31561 gccaggtgag tttgtcatgg gccgaaatgc cggtataaat caggattaac gttagcgcca
    31621 gtgcgccggt gttaagaatt aacggcttga gtgtgcgggt catggtgagt tcgcaagtca
    31681 gggaaaatac tgactattca ccagcaagcg tgaaattaca atcgcaaaag aggtggccca
    31741 gggggatcac ctggcagcat gctgccaggc gctgggccga agaggttact tagtgcagtt
    31801 cgcgcactgt ttgttgacga tctgctggaa gaagtcgtta cctttgtcat cgaccaggat
    31861 aaacgccggg aaatcttcta cttcgatttt ccagatagct tccataccca gctccggata
    31921 agcgacgcat tccagatgct tgatgctctg ctgcgccagt accgccgccg gaccgccgat
    31981 gctaccgagg tagaagccgc cgtgtttatg acacgcgtcg gtaacctgct gactgcggtt
    32041 acctttcgcc agcatgatca tgctgccgcc gtgggattgc agcagatcca cgtaggagtc
    32101 catacggcct gcggtggttg ggccaagtga acctgatgga taaccggcag gggttttcgc
    32161 cggacccgcg tagtagatcg ggtgatcttt gatgtactgc ggaagttctt taccggcgtc
    32221 aatcagctct ttcagcttgg cgtgtgcaat atctcggccc acgataatgg tgccggtgag
    32281 cgacaaacga gtggataccg ggtattgcga aagctgggcg aggatctctt tcatcgggcg
    32341 gttaaggtca actttcaccg cttcgccttc accggcctgg cgcagttctt gtggaatgta
    32401 ctggcctggg ttgtgttcca gtttttcgat ccagatacct ttgcggttga ttttcgcttt
    32461 aatgttacgg tcagcggagc aggagacgcc catgccgacc gggcaggatg cgccgtgacg
    32521 tggcagacgg ataacgcgaa tgtcgtgcgc gaagtattta ccgccaaact gcgcgccaag
    32581 accgagtttc tgggcctctt ccagcagttc ctgttccagc tggacatcgc ggaacgcctg
    32641 accatgttcg ttcccttccg tcggcagttt atcgtaatag tgagcgcttg ctaacttgac
    32701 ggttttcagg ttggtttccg cagacgtacc gccaatcaca aacgcgatat ggtacggcgg
    32761 gcaggctgca gtaccgaggg tacgcatttt ctcgacgagg aagtttttca gtttgccggg
    32821 agtcagcagg gctttggttt cctggtagag atacgttttg ttggcagagc cgccgccttt
    32881 cgcaacgcaa aggaatttgt actcatcgcc atctaccgcg tacaggtcga tttgcgcagg
    32941 caggttagtg ccggtgttga cctctttgta catgtccagc gccgcattct gtgaatagcg
    33001 caggttatct tcgatatagg tgttatagac gcctttcgac agcgcttctt catcaccgcc
    33061 gccggtccac acgcgctggc cttttttacc gacgatgatc gcggtgccgg tatcctggca
    33121 ggtcggcagc acgcctttgg cggcgatttc ggagtttctt aagaattgca gcgccacgta
    33181 cttgtcgttt tcgctggctt ctggatcgtg aagaatagcc gcaacctgtt tctggtgtgc
    33241 cgggcggagc ataaaagaag cgtcgtgaaa ggcttgctgc gccagcaggg tcagggcttc
    33301 tggttccact ttcaggatgg tttcgccgtc gaagtcggca acgctaacgt aatcggaagt
    33361 gagtagatag tattcggtat tgtctttccc catcgggaaa ggtgcctggt agataaaggg
    33421 tttgtttgac atagcttcca gcctgtaact ctgtatttgt tattcgaaaa tggcgtgccg
    33481 cgtagtcacg ggcggcacgc aaagtgcatt tataagaacc cgtacatcgc ggcgaagatc
    33541 cagccgaaga cgcacgatac gctcacacca atcaaccccg gcagaataaa gctgtggttg
    33601 atgacgaagc gaccgatgtg ggtggtgccg gaacggtcaa actgaatcgc tgccagatcg
    33661 ctcggataag tcggcaggat gtaataaccg tagcaagccg gtgctgaagc cacgatgtat
    33721 gccggatcaa cgccgatcgc cagcgcgacc ggaacaatcg ccgccagcgc cgcagcctga
    33781 gagtttacaa acttggaaac cagcagcaga acaatggcat aggcccacgg atactctttc
    33841 accatttcac ccagtacgcc ctgaatttca gacatatgcg caccgaacat ggtttctgcc
    33901 atccatgcga taccgtacac cgccacgatg gcgatcatac cggaacggaa gacttcgttt
    33961 tttgagatag acgcgggatt ggttttggtc aggataataa tcagcgcccc ggtcagcagc
    34021 ataaacatct gaataaccag taccatcgac agcggtttgc cgccgaagga tggacgcagg
    34081 tccgaatcag caccaagaag ggcgactaca gcgattgcac cgaggaaaat ccacattgcc
    34141 agccagttgc ttttcggcag ttttttatcg agcagcgtcg cggtatcacc gtaaacatac
    34201 tcacggtttt ccggtacgga gatgaatttc tggaactctt cgtctttatc cagatcttta
    34261 ccgcggaacc agctgaagat accgatcgcc aggataccga ttaacgtcga tggaatggtg
    34321 attgccagca ggtcgaggaa ctcaagatgg cgaccatcaa aggtgacatt acccagcatt
    34381 gcaaccagag acacgaccgc aaccgacacc ggactggcga taatccccat ctgtgcaccg
    34441 atagaacttg ccgccatcgg acgttccgga cggatgttgt tcttaatggc gacgtcgtag
    34501 atgatcggca gaatggtgta aaccacatga cccgtaccgc aaagaatggt cagtgtacag
    34561 gtcacaaacg gcgcgacaat tgagacatat ttcgggttgc ggcgcagcaa cttctcggca
    34621 atttgcagca tgacatcaag accgcccgaa gcttgcaagg tcgccgatgc cgccaccacc
    34681 gcaatgataa ccagcatgac atcaactggt ggtttacctg gctgaaggtg gaagacgaag
    34741 accagaatga ccagaccgat accgcctaat aaacccagcg cgataccacc ctttctggca
    34801 ccataaaaca gacatatcag tattatgata agttggatag taaataacat gtgtgaaccc
    34861 tcgcgataat cctatttaaa tttttgctga atagatcaca gtcacgttct gttttgtatg
    34921 aactgttttc agagcctgac taaatatccg tttggtcgtt accggcttta gcaaatgcct
    34981 cacagtgaat attggctgat taaagcagcg gcaaatttat gcactgtgat aagcggcttt
    35041 ttcagagaga aaaactctct ggcgatgggt ttatagtttg tactgcgcag atgttaatgt
    35101 tttgtttgct gttatcttat tgatatttat cggtctattt tttgttctca gggcagatat
    35161 cattaggttt aaagattaat ctgatctacc catttgtggg taaaaataca cataatgcgg
    35221 gtgacataat agttaattaa cttttgttag cgttttgaaa ttaaaaacac cgttcacctg
    35281 aagagatatt aatttttagc gatgatggag ggataattat atttgatctg gcacaagttt
    35341 tactgatgaa ggatgtaact tgtgccaggg gtattttgca ttacggtaat aattattact
    35401 tacagataac cagcgatccg ttattggcaa tattgtttca gtagtgagta gtgttctgcc
    35461 tgaatacggt aacggtaaac tggacgcccc gtgacgccat aatggatact ggtgaacaag
    35521 atgtggcagt tgaccagcca gatgaggtat ttacggcagg aaacacgcga aatgttaacc
    35581 tcgttggcta gctcgtcggt tgaaaactca tagtcctgat gcgcgtcaat ccactggcac
    35641 agtgtgcgta acgtctgcgg cgttaagcct tttggcaagc gacgaggatc ctgttcgttg
    35701 gagctgctgc cgtggattag ctgatcaagc tcggcctggt cataatactg atgtttttcc
    35761 agcgccattt tcttttgccg ccagccggtg agcgcctctt caaagcggga agcctggaag
    35821 ggtttgatca ggtaatccac gacaccgtaa tgcagcgaat ctttaatggt tgccgcatcg
    35881 gctgcggagg agatgacaat cacatcactt ttgcaacgcg cgttatgcag gacaggcagt
    35941 aaatcgagcc cgttctcttt ttgcatatag atatcgagca atatcaggtc gataggcgta
    36001 tcgctattga agatcatctc tttggctttc tccagcgtcg aggctgttcc acagcattga
    36061 aagcctgaga tttgtgctac gtatcggcga ttcagctccg cgaccattgc gtcgtcatcg
    36121 ataattaata cattgatcat ctgttcgacc tctccccgtc ccagggtatc tggacaaaaa
    36181 attgtgtgaa aatcccgggt tccgattcca cggcgatgct gccgccgaga ttttctacct
    36241 gttgtttgac aagtgctaaa ccgacgcctc gctcgcttcc ttttgtcgag acacctttgt
    36301 caaaaatgtg atcgattttg tcgggtgcga tccccggtcc atcatcgtta acttcgcagt
    36361 gcagccagcc gtgacggtag tgcaatgtta cgctaatttc gccaccgggt tccggcccta
    36421 atgcttccag cgcgttttct atcagatttc ccaatgtggt aatcagcgtc gcgacctggt
    36481 cctcactgcc gctgtctggc agctggcttt cactgtttaa aatcagcgta tggcctaaat
    36541 cggtcgcgcg attaatcttg ctgattaaaa aaccagcgat aaccggagat ttgatcttac
    36601 ccagcagaga gccaatctct tcctgatagt tattggctgt tttgagaatg taatcttcca
    36661 actgcttata actcttcaga tgcaataatc cgagaatcac atgcaattta ttcataaatt
    36721 cgtgggatcg ttcacgaagt gcatcagcat agttgaccag accgtcgagt cgctgcatca
    36781 gtttacgaac ttcagttttg tccctgaagg ttgaaatggc accgatgata acgccattac
    36841 tgcgcaccgg aacggtgttg atcagtaata gccggtcttt aatcgtaatc tcttcgtcgc
    36901 ggcgcggcgt accgtcgcgt aacacttccg agacatctac cacctgtgac catgagtggc
    36961 ttagcgtcga cagtttctca tcgtcctgcg acttacggta attcagcaat tcttgtgcgg
    37021 catcgttgat cagcgtgacc tcgccgcgat cgtccacggc aacgacgcct tctttgatag
    37081 actgcaacat ggcctggcgc tgctcaaaca gcgtggagat ttcgtagggt tccaggccga
    37141 aaaggatttt tttcagtacc ttaaccagaa tgcaggtgcc aatcagtccg accagcatgc
    37201 caaataatac cgaccagata atgctccagc gactgtcatt gatttgttgg gtcacgcggc
    37261 ttaactcaag gccgatcgcc accacgccaa tttgtttatg attttcatcg tagatggggg
    37321 taaatacgcg taaagcctgc gccagaaaac cgcgattgat agcgacattt tctttgccat
    37381 tcagcgcttt aaggatgtca tcacctttaa atggctgacc aatacgctgg gcttcaggat
    37441 gcgagtagcg aagactttgc atatcggtaa cgacaataaa cagcagatcg ttgcgtttgc
    37501 gcacggcttc cgcgatggcc tggatgccgc tctcctgcgg ttttttctgc aagccctgac
    37561 ggatttccgg cgagtcggcg agggtacgcg ccactgccag tgccttgttg gctagcccgt
    37621 ctcgcgtcat atcactgatt tgcgagaagt aaatcagatg caccaccaat agcaccgaga
    37681 acagtaccgc actgaccatt aagatcactg tggtactcaa tttcatcgga cgtttgcgta
    37741 acatgcggta gggcaatgaa tgtctcatca gcttccttgt gtgacaaatt tcttaagcat
    37801 tatctctgat gaggcgggta attcaaaggg agtaagaatg attggctata taggggaaga
    37861 gattctggca acggaaactg ccagtgctgt atgaagatta cagggctatg cttatagcga
    37921 taatcatact gatgagagag ggaaagtcat ggatcaggcg ctactggacg agggttatcg
    37981 ctgttatacc ggcgaaaaga tcgatgtcta tttcaacact gcgatatgtc agcattctgg
    38041 caattgcgta cgtggcaacg gcaagttatt taatctcaaa cgaaagccgt ggatcatgcc
    38101 ggatgaagtc gacgtcgcca ctgtggttaa agtgattgat acgtgcccga gcggtgcgct
    38161 gaaataccgt cataaataag cgagggtaaa atggaaatac gcgaaggcca caataagttt
    38221 tacattaatg acgaacaagg caagcaaatc gctgaaattg tctttgtgcc gaccggagag
    38281 aatttagcga ttatcgaaca taccgatgtc gatgaaagcc tgaaagggca ggggattggt
    38341 aaacagctgg ttgcaaaagt cgtggaaaaa atgcgtcggg aaaaacgaaa aattatccca
    38401 ttatgtccat ttgcgaaaca cgaatttgat aaaacgcggg agtatgatga tattcgcagt
    38461 tgatgggaga gtacagattc acgatatttt tcatgctctc cgcggtgtga tgcaggagag
    38521 catctgaagg gtagggggat gcacaaagaa tgggcagaga gagcgttttt ttgtcccaag
    38581 tcatcccctt tactgagcaa aaaaaagaat atctcctata tgagaatcat caatcggggt
    38641 taataagttt tgcgtcccca gagcgttcaa tattgatagg agtcatatta tggaaggtaa
    38701 aaacaagttc aatacttatg ttgtttcttt tgattatcca tcatcttatt cctcagtgtt
    38761 cttaaggtta agatcattga tgtatgatat gaatttctct tctatcgtgg ctgatgaata
    38821 tgggatacca cgacaattga atgaaaattc cttcgcaata acgacatcat tagccgcaag
    38881 tgaaatcgaa gatttaatca ggctcaaatg cttagactta ccggatattg attttgacct
    38941 caacattatg acagttgatg actatttccg tcagttttac aagtagccag gaaggataag
    39001 aagaacataa aaaatggcac tattctctaa aatattaatt ttttatgtga ttggtgtgaa
    39061 catatccttt gtcattatct ggtttatctc acatgagaaa acacatattc gtttacttag
    39121 tgcattcctg gtcggaataa cctggccaat gagtctgcct gtggcattac ttttttctct
    39181 cttttaggag ctgtagttga gtggacccgc tggttatcgt gaaacgaagc gtctctcctc
    39241 aaaactggat ttgcggaacg acgaacgaag cagtcgcctg actgcttcgt tcattaaagt
    39301 gaaatttatt tctgtgggcg catcgccggg aagagaataa cgtcgcgaat agtatgactg
    39361 tttgtaaaca gcataatcat tcggtcgata ccaataccca gaccagcggt tggcggcaga
    39421 ccatattcca gcgcagtcac gtaatcttcg tcatagaaca tggcttcgtc gtcacctgca
    39481 gctttagcgt taacctgttc ctggaaacgt tcagcctgat cttctgcgtc gtttaattcg
    39541 ctaaaaccat taccgatttc acgaccaccg atgaagaatt caaaacggtc ggtgatttcc
    39601 gggttaacat cattacggcg cgccagcggt gacacttctg ccggatattc cgtaataaag
    39661 gttggctgaa tcagatgtgc ttctgccact tcatcaaaga tctccgtgac aatacgtccc
    39721 agcccccagc ttttctctac cgtaattccg atagattcag ctaatgcttt agcagcatca
    39781 aaattatcca ggtcggccat atcggtttct ggacgatatt ttttgattgc ttcgcgcatg
    39841 gtgagttttt caaacggttt gccgaaatca aacacatgct cgccataagt gactttagtg
    39901 gtacccagaa cctcttgtgc cagggtgcgg aacagggact ctgtcagttc aatcaaatcg
    39961 tggtaatccg catacgccat gtagagttcc atcattgtga actcaggatt atggcgaaca
    40021 gaaatacctt cattacggaa gttacggttg atttcgaata cccgttcaaa accgcctaca
    40081 accagacgtt tcagatacag ctctggcgcg atacgcagat acatatctaa atccagagca
    40141 ttatgatggg taataaacgg gcgagcagat gccccacctg gaattacctg catcatcggg
    40201 gtttctactt ccataaagcc gcgcgcgacc atgaactgac ggatagcggc cagaattttt
    40261 gaacggacaa cgaacgtttg acgggattta tcgttagcga tgaggtccag ataacgttga
    40321 cgataacgga cttcctgatc ctgcagacca tggaatttat ctggtaaagg acgtagtgct
    40381 ttagtcagca ggcgcagctc agtacagtga atggaaagct cacccgtttg cgtcttaaac
    40441 agcgtaccgc gggcaccgat aatgtcaccc agatcccatt ttttaaactg atcgttataa
    40501 acaccttctg gcaggctatc tcgtgcgacg tacagttgaa tacggccacc gacatcctgc
    40561 aacgttacaa aggaggcttt ccccatgata cgacgggtca tcattcggcc agcaaccgag
    40621 acttcaatgt ttaaggattc cagttcctgg ttatccttcg catcaaactc ttcgtgcaac
    40681 tggtcagagg tatggtcgcg gcgaaaatca ttgggaaacg ccacaccttg ctgacgtagt
    40741 gccgccagtt tttcgcggcg atttctcagt tcatcgttaa aatcaatagc ctcattggct
    40801 ccccgtgttt cttgttcaga cattttggtt cctctaaatc cagctttcaa tttagcgtag
    40861 ataaagagac agatcggtac actgtattat ctgcctcgac tattaataac tcagtttatc
    40921 ttatcgaaac taacgcttac ctcaagttga tatcactaat aaataatcat caaaatggtt
    40981 taaatgactt attaatctcg atttaatagt gtgataaact gaaagttaat ttattcataa
    41041 cctattgttt ttacgacagt taatcgttgc tctcctgtat catattcgtc ggcgtgctaa
    41101 acagaaaacg ggtagcaaag gccaatacca cgatgatcgc gacacaagcc aacgtccact
    41161 ctcccatctg agaaaagaat cgctggtaag ctgcaattgc cataccgcta atttgcgact
    41221 ctgtcgtctg ctgtgccacg acgcctgcca gccagttggc gaccgcgcct gttgccagca
    41281 tataaatccc ggttaatacg ccagacattt tcagacgcgt gatttgtgca atcgccaccg
    41341 ggtcaataaa gagttcggca aagcccatta gcgccaaccc ggatatcatc acgcccattg
    41401 acgcttgacc gtcagccgct gcatgtcggg catcaaatgc caacaacata aagccacaag
    41461 ccatcagcag taagccaaag gcaaacttca gccagacgcg caatgttgag ttgccgcggc
    41521 tttctggcga cgccagccag gccagtacaa ccccagcgag catcaccgca attgcattca
    41581 ccgactggaa tagtgctgta ggtacttcaa tattgaatgc ctgacgattc acaaagcgat
    41641 cgataaacaa gctgatggta ctgccgccct gttgtgccag tacccagaac aacgtcccga
    41701 caaacatcaa caatacaatt tgccaaagag cacggcggtg ttcggggaat ttgatcatca
    41761 tgcgggcaat gatttgtgcg gcaatgaggc aaacgatcgc cagcaaatat ccggaccagt
    41821 cgttctccag cagcagggta aaaaatactg gggctaaaca gagcatcacc accaaccagc
    41881 tccataccgg taaggcaaat ttgacgctag tgagcgcttt tttatccata ctacgcgtgg
    41941 actggaaatg acgatgaccg cttaagaaaa tcaacaaacc gataaacatg ccgccacccg
    42001 caagggcaaa gccaacatgc catccatacc actgagcagc caggccgcag gcgattgggg
    42061 ctgcgataga accgatattg cccgcagcat acagcagcga aaaaccgcca tcacgtcgat
    42121 gatcgttctc gtcgtaaagc tcgccaagca aacagctgat gtttgatttg aataaaccgt
    42181 agccacaaat aatgattgcc agcgccagat acaggctaaa ggttgaattt gtatcaatgc
    42241 ccagcaccac atgaccaagg gtcattaaca gcgcgccggc aatcactgca gtgcggttgc
    42301 cgagcaggcg gtcggcaagc cagccgccga gaataggggt aacgtaaacc agagaagcat
    42361 atgcgctgaa caggctgatg gcatggttat catcaaaacc aagctgatgg gtgagataga
    42421 gaatgagtaa ggcacgcatg ccgtaaaaac tgaagtactc ccagatttgg atcgccacga
    42481 tatagtatat cgcgcgcggc tgtgagggtg ttttcatgtg ttctccttat gagcaaaaaa
    42541 gggaagtggc aagccacttc ccttgtacga gctaattatt ttttgctttc ttctttcaat
    42601 accttaacgg tatagcggcc atcagcctga cggtatgcac cgtgaatatc agtttcaaag
    42661 cccggatagt gagcgccgat ttcacacagc atctgcagga actccagaac cggacggctt
    42721 tcttcggtga tcatttcacc cggcattacc agaggaactc ccggcggata cggaaggatc
    42781 atattggcgt taatacgacc gaccatttcg tcgaggtaaa cttcttcggt cataccgtgc
    42841 agctctttct ggaacgcagc atacggagtc attaccatcg tcggcagcac ttcaaatgcg
    42901 cgatacatca gatccggcag attgtggtga acaatcagtt tgtggatatt ctgagccagt
    42961 tcctgaatac gcatgttttc atagaattca ggatcttcac gatacagaga cggcagcatg
    43021 tttttcacac gcaggttcag gtcgaacgca cgtttgaagt cagtcagagc acgcagcaag
    43081 ctcagtgctt tggtcttatc gataccgatg ctgaacagga acagcaggtt atacggaccg
    43141 gttttctcaa caacgatgcc atgttcgtcg aggtatttcg ccacgatgct ggccggaata
    43201 ccaaagtcgc tcatggtgcc gtctttttcc atccccggag tcagcagggt gactttgatc
    43261 gggtcaagat acatgtgctc gttatcgatg tttttgaagc cgtgccaggt gctgtcagaa
    43321 cgcagcggcc agcattcagt cgtatcgata tgatccggct gccaaacatc aaagaaccag
    43381 ccatcagatt ccgttctcag acgtttgatc tctttacgga atttgatcgc acgttcaatg
    43441 gaaccgttga tcagacgctt accagcatta cctttcatca tcgccgcagc ggtttcagtg
    43501 gacgccacga taccgtagtg cggagaagtg gtggtgtgca tcatgtaggc ttcgttaaag
    43561 gtttcttcgt ttacgtcacc tttaacgtgg atcatggaag cctgagagaa cgccgccagc
    43621 agtttgtgag tggactgggt ttcgtaaatc actttccctt ctacacggcc accgctcata
    43681 ccgcatttac cttcgtaaat cggtgagaag ttggtgtaag gcacccacgc ggagtcaaag
    43741 tggatggatt tcacatccag tgttttcttg atgaagtcgg tgttgtacag cagaccatca
    43801 taggtagagt tggtaattac agcatgtacc ggccaggttg cgtttggtgt ttctttcacg
    43861 cgcttagcaa tggtagcgtg ctggaattca ctctgtggga taccaccaag aataccgtaa
    43921 gcgttacggg tcgggcggaa atagattggc gtaacatcgc tcatcatcat caggtgggtc
    43981 agcgatttgt ggcagttacg gtcaatcaga atggtgctgc ctgccggagc agagtacatg
    44041 ccaacaattt tgttcgcagt ggaagtaccg ttggtcacca tgtagctgcg gtctgcgtta
    44101 aagacgcgag cgatatactg ttctgcttct ttgtgtggac cactgtgatc cagcagagaa
    44161 cccagttcag atactgaaat ggaaatatca gatttcatgg tattcggacc aaagaaatca
    44221 tagaacaggc tacctaccgg gcttttctgg aatgcagtac cgcccatgtg accaggagta
    44281 cagaaagtat atttaccttc acgaacatat ttaaacagtg ctttagtcag tggaggcaga
    44341 atagtgttga tatattcgtc agtggtctgc ttgatcttat tagcaatatc ttcagcagca
    44401 cccagcgcat attcaaagaa gctaatctgt aaacgcaggt cattcaggct tacatcgaga
    44461 gtggaatacg tattagcgaa cgcgtacaac ggcaggttct cgttcatttt gctaatttct
    44521 tcgcacagct cgagattata tttatcccag tcaaaaataa cgccgcacag acgcgcattg
    44581 ttttcgatca gttttaataa gtcgtcacgg tcgttcgggt aaacaatctg gaagttcaga
    44641 cgttcaagcg cgcgatgaag ttcacggatg ggttcttctt taaaataaac ccccatgtga
    44701 ttcaatattg caataacgtt catagtcata tctccaggta aaaaaggccc ctcccaacac
    44761 atgggacaaa atgaaaggag gagcctcgga aaatactttt aattaatgtg cgttagacgc
    44821 ggtgtggtta tccattgagt ggctctggcg ctcgtgcatt ttgcgagcgt agaacatcag
    44881 gataatcagg ctgacgatga aggtacctgc cagctcgaag gagcttgcgc ccatcagcgc
    44941 gatgaagcag aacacacaac ccagtacaga gcagatcagg ctgacaaagt tgcggatgtt
    45001 aacgccttca aaacgaatca ggtcaacgca agagtagaag tacggcagca tagtcagcag
    45061 tactgcgata ccggtcagtt caccgaacag gtcagatgct ttaccaccgg cagagttcat
    45121 cagagtgatg aggatcatca gggcagtcat tttcactgca gccagcagca gacctttttt
    45181 cggaataccg ttgctgtcga cttcaccata aactttcggg aagttaccgt cgttagcggc
    45241 acgtacacct gcctggccta ccaacatcat ccaggagccc agagaagtca ggcacgcaaa
    45301 ggcggtgaat gcagaaacca gcggcgcagc ccagttaccg aggatggttg aagcactgat
    45361 tgcaaacgga gcaccggaag ctgccattac agaagatgga tacataccgg aaagcacctg
    45421 agtcgcagcg atgtaaacaa tacctgctaa accagtaccc agcatggttg ccagcggaac
    45481 ggtacgtttc gggtttttaa ccataccagt acttactgct gcggattcaa cacccacgaa
    45541 ggcccacagg cagagcagaa tacttttaat gatcgcatga ccatcagtgg tatccgcagt
    45601 attccagtta gctgcataag ttgccgcatc aaaccaatgc cagccaacaa tagcggtcat
    45661 tactaccgga ataagaacca gcaccagacc aatagtggtt aaacggctta cccaagtacc
    45721 gccgagcata tttacaaagg taaataccca gacgatagca atacaggcga tacccgccgg
    45781 aacaggatca tttaatactg ggaagaaggt ggaaagataa gataccgcgg taataccaat
    45841 cgccaggtta ccaatccagt tagcatggta ataaagaaca cctgtctgaa aaccaaatgc
    45901 aggggaaatt tctccggcat aagcaattgg gccaccttgt tgcgggtttt ttgttgccag
    45961 tcgggcatat acatacgcca gcgacattgc accaataata gagataatcc aaccccagat
    46021 agcaatacca ccgatacttg ctaggttcgc aggtaataat gcaataccgc tccccatcat
    46081 attaccggca acaacaccgg tacaggcaaa tagcccgatc ttcttggcag aactcatgct
    46141 cttctcctaa tttcattttt gaatttggag tccgggtcat gatgtataac tatttcctga
    46201 ccagaccaaa ctggcgctaa gaatactcac gaaaaaagga ttaatcctaa agattaggtg
    46261 aaataacaca aaagtttctg taagtgagaa cttgaggttt tttattaaca catcaggatc
    46321 gcaagttgat atcatgaaaa gataaacatt caatgtttac aatggattgc gggatattct
    46381 ctggttaaat ttatgtaata aaaattatgc ggcaaataaa ttgccgcaac atattatacc
    46441 aacagaaaca tacaaaaact caacaacaaa tattcccgag cataaatcaa cccgggagtt
    46501 acttattctg aagcgagaaa tttgtcgaga taaggtacaa cataaggaac agaagtctgg
    46561 aatataccat tttcaatcca gtaaagggtg tttgcccctg ggcgtaaatt aaaggcggtg
    46621 agatatgcat cagccgcttc ccggttcatc cccttcattt cataaacctt gccaagcaac
    46681 acataattta gccaggacat ttcaagatca atgccagtat ttatcgcctg gtaagactca
    46741 tctgttttac cttttaccag agcactaacc gcttttattt gatatataat ggacaggtta
    46801 ttcaattccg gcagtgtaac aatgttgtct atttctgtgt tcagtgctgc taattgtttt
    46861 tcatctaaag gatgttgaga atggcgcacg atatcaacta atgctttttc tgctctcgcg
    46921 taggtaaatt ctggggatga ttgaacaatc tcacctaata attcactggc acgattcaat
    46981 gatttgtcat cgccatgcag taaataatca tgagcctgat aaaaattagt taataacgca
    47041 ccacgatgag gcaaaatttg ctggagcgtc tcctgcattc gttgtggcca cggttggttt
    47101 aacgcttttg ataaactctc cagtaaatca ttttgaatcg ccagctgatt accgttagtg
    47161 atgacataac gtttatccag catggttgaa ccatctgcat tgtctaccaa ttttatcgac
    47221 ataaagcatt gttgagcacg gtattggcgc tgattaacaa aggcaataga taatgtttta
    47281 ccggaactgc tcggttcatc aatgttgtag ttgattttgt catgcaccat aaaggtggag
    47341 aaggtgttaa gtgatgtcgc caccaaatca cccacgccta tcgcgtaaga gagctgatac
    47401 ggggaactcc agctgttaca acttttattt accatattaa tgtcaatatc gcgtggattg
    47461 agcaaaatac gcgatttgct cataggaaga cgtgtatcaa gagttgaaaa cgctaccagt
    47521 gctacacaga tacctaacga caacaggaaa aaaaaccata cccaaaaggt agtgaatcgt
    47581 ttgcttttaa ctggggattg ttcaggtggc gttgcggtgt tttgaatgtt aagactgtgg
    47641 gagggagaat ctgtggcagg aaccgcctct ggtatagggg gaggcgaaga tagcattatt
    47701 tcctctccct cttcttcgct gtaccagata accggcacca ttaatttata gccgcgcttt
    47761 ggtacagtag cgatatagac aggactatct tcatcattat cttttaatga cttacgtagt
    47821 tctgagatac tctgcgtcac aacatgattg gtgacaatac ttctcttcca gacattatcg
    47881 ataagttcat ccctgctaag tacttcgcca ctgtgttgag caaagaaaac cagaagatca
    47941 attaatctcg gctcaagggt aagttgacgc ccattgcggc taatttggtt tatggacgga
    48001 gtaacaagcc attcgccaac gcgaactaca ggttgttgca taatagaaac tcattcgaaa
    48061 agggaatggt gcaatgataa ttgccatgac ctatttttac catcaataga tgggtttatt
    48121 tacatgttat tggtgaatgc aagacgttat ttttaccagc cataaacttc ttgattacat
    48181 agtattacga aaggatttta ctgagaacca gaagtaatat tccttaccat caaaattcat
    48241 catctttgcc aaagaaaaat gttcagaaaa taatccatgg aaaattgtcc ggagcgctta
    48301 ctattttaat ggattgttag tctttgcatg agcaagcgga ctgatacatt tctctttgtt
    48361 ctcattcaga aaatctcatc agtcggcgtt cagaccggtg ttgtgcttta tggatgcgtt
    48421 acgctcctga tgacgtcatt tgacgttcaa cagcatcacg gggccgcacg acatttcacg
    48481 tcagttagtg ctatagctca ggaacaaatt ttcccaaatt gggatatgct cgcaaattgc
    48541 tggtgatgtg gggagaatct ggttgagttc ggtagaattg atttggaggc agaacgctta
    48601 aatcgtggcg tcctgaaacg aaaaacggac ctccgtggag gtccgttgat atgaatttgg
    48661 tgcccggact cggaatgaaa ctggttatat aaccaattgt tttgtatcga ttgttttgtc
    48721 tttgttgttt tcgaggccac caatgaggcc cccatgtgca attcgaattc ttttgacgct
    48781 ataatccctc ttttttgtgc tttgctgtat cattttgtct ggttcgatat gctgaattgc
    48841 ggaatcgaag taacaacaaa atacagagat ttataaaata cctggctacc tgtgttaggg
    48901 agcgttaacg cacagatttt gtttatggct gccagaaatg atctgatggt ttgtcgtctg
    48961 gctctgcaag taccctgttg atatcccggg aaccatgaac gatacgttcg atacgaactt
    49021 cagtatccag taccctgaac agcagaaggt aacgaccata cgaacaacct ctgatcccag
    49081 cccctaattc gggtctttct ctgtagagta ctggcgattc ggctatcaac agacattgtt
    49141 ggtaaagctc ttcggtaaaa ctgattgcac ggacaggatt atcctgagcg atccagtcac
    49201 ctatagcttc aagatcgtgt tctgctaatg gtgaaacagt cagtttcata ttggctgttc
    49261 accttctgcc atctttttgt atttgtgcgt cagtcgcccg aatacctctt cagcactttt
    49321 actttcaccg ctgttgatgc ctgcggtaac ggcgctctgg agtgattcaa gctttatctg
    49381 ctgttcccgt tcttccagag cacgaagtcc ggcacgaatg acttcacttg tattgttata
    49441 ccgaccactt tctatttgtt cgcggataaa cgtttcaaag taaggactga gggatacact
    49501 ggtaggcatg gtgattttct cgccgattga ttgctatcaa ttaataatag ttaatattcg
    49561 cgaattgtaa aatacctctt ttatgccttg cggcttttgt tcactgtgct tgccagggct
    49621 tccgggtagt gtctggcaat aatatcatta agcctgtcaa ccagttctgg tttcctgaac
    49681 gtgatgtgta ctgagccttt cttaaagtat ttaatactga acatctcgtc ttcatagtcc
    49741 tctttaccct gaacagactg gatatgatca tccagacgaa gggtgatgtt ttcccggttg
    49801 tcggggctcg gtttgccgct gaacaggtgt agcattcttt ccagatcggc aagtcgatct
    49861 gcctgttgcc cggtgataaa atgaaacccc caccggtccc acctcaccag gttgttgacg
    49921 ataattttac tgccaaattt acagggtgag ttggttttgt aattccagct cagtcccctg
    49981 aagacgttga tcacccctcg ctcaaacacc tcatccttat tctggtgcag ttgttcaaat
    50041 gtgctcagaa tgtttgccgc actgatttcc ggaaaattat cgtattccag tgaccggtac
    50101 catgtatcac gtgcctgtgc atccattaat gacagcatcc cggaacgttg catcaggtca
    50161 cgccagattt cgcggtcaag attccgggta atggctttca ttgctgtttc gggtttctcc
    50221 atcagccagc aaccgcagcg aaaattctgt ttcatggccc agtccggggc ggttttacct
    50281 ccaatacttt ctgtcattgc tgagatttct gtcagttgcc ggataagtgt gtcgatttgc
    50341 atcagtgtgg tctttcgtac agccagaatg tgtccgattg aggtggaaca gataacgtcc
    50401 gtatgcccgt tgaccacttc aggacgggag attgtgatac ctgacataat cgtttttcct
    50461 ttttgcagat aaatgaaaac gccagccctg ttcaggctgg cgtcagtgga atgaagtatg
    50521 gtgagtgata tttaccggtt ctggtgcaga agctgcagat gacgaagagt acggacatcc
    50581 tcccgggcct gttcgataca gggcaacagt gctgccgaat ccgttttatc ctcatcgttg
    50641 tcaaagataa ttcccgattc gcagtcgata ttgtcctgca gccacgcaat gaggatatcc
    50701 agcgctgttt cagtggttaa tgatttcatg ttgtttctgg ttaccagttg aaagtgggta
    50761 aacccggcag gcattcagcg ctggcatcca gatgaatgag actgacgcca taacgccgga
    50821 taagtgtgac aaccagacgg cggaacattt ttgacagccc caggcgttta agccgcagaa
    50881 ccgggtatga ccacgcatcg gtacgtaaca gatatccgct acctgtaaaa tgaatccatt
    50941 ctgattcgcc gaagtcactg gtctggtgtg acagcgagta caaccaggcg ttgtctgttt
    51001 ccgtgatatg tgcggtgctg cagcgtatgc cggtaagggt tgttaacggt ggtggtgtgc
    51061 aggttgactg ttggtcggag tcgtccacca catggagtga ataaccgttt tcagcgactt
    51121 tgttaatcag ttcagcgagc tcaatgccat cgacgtcaac gacaatgcgc cccatgttca
    51181 gtgcctgcac gttaatgctg tcggcttcca gtgtcagggc cagtttcatt gttgcgcctc
    51241 cggatgctta ccctgggtaa tgttatttac cattctgtaa ttgtcgcggg tcatcaggcc
    51301 tgttgcccgg cgagcccgga ggatatcgat gctgtttatt aactgagagc gggtacaggc
    51361 gctgaatcct ggctggtcag ttcgtaccag cgcgtatttt tccacgagaa agttcaccgc
    51421 atcacacagt gaaatgcctg cctcaatatg ctgctcaatc acacgttcat cggcaaacgg
    51481 tgtgtcattc agtgtgaggc cataatgctg gtccagcagt cggatgagca gtgtctgcca
    51541 gatggtgacg ggagacgggc agcgagatgc ctcccgtaca tgtgtgtcgg ataatgtttt
    51601 catcttaagt tctctgtaac tggtagtgct aaaggtgaga gcttcggggt tatgaggtgg
    51661 ttgcggttgc cggtgtcgga taaacagtca gataaacgta accacaggag ccgagggtgt
    51721 cggcttcgca ggtcagccct tttgcataca gcgtgacgga atgctgatgt cggggattca
    51781 gttcaccgct ggtgagcatg agttccagtt gtttcatcag cagcggaaag gcctggtcca
    51841 gatggtatgc atctgcgtcg ctgaagcggc cgctgatacc ggcgcggtcg gcaaggtaat
    51901 gcaaccggtt accctcctgc accagacggg cgccaaaaca gggtttcact gtgcagggaa
    51961 gcccccacca ggggcggtcg tgattgtcgt cgggatgagt tatcccggag agtttgtctg
    52021 acacgataaa atcctctctg aaaagtggtt aagaatggtt taacgatgac aggtcagccg
    52081 gggcactgac cggtttcggt gaagatgtat gcattgatga gaatggcctc atccacagcg
    52141 gcgtctgagg tcatctggtc gtactcgttt cccgggtgac ggaacatcag ttcagggtga
    52201 cgctcatcag ccggacatac gggccaaaac tgtccttacg gcgttcggca aacacggcca
    52261 gcacaccggg aatatcctgt acttcacgac cggtatacgc ttcagtgctg ccttgccagc
    52321 ggtatttgcc ggtgcagaac ggaaaaagac gggatgcagg atgctgccgg tgaatgcgca
    52381 tggcttcacc acgggtgata attttcataa tgggatacct ctgaagacag aagataaaag
    52441 tgaaaacagg tgtgatgtgg ttgtgacggg ttaaagcaga ccatgttcgg caaaggagaa
    52501 aacctggctg ccaccgacta tcagatggtc cggcacccgg atatccacca ggcccagtgc
    52561 ctgtaccaga cgttccgtga taaggcggtc tgccttactg ggggtgactt caccggacgg
    52621 gtgattgtgt gccagcacca cggc
//
LOCUS       DAZGAN020000022        51473 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGAN020000022 DAZGAN020000000
VERSION     DAZGAN020000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 51473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.022"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            143..901
                     /gene="ydiY"
                     /locus_tag="ACHKYO_RS07440"
                     /old_locus_tag="pgaptmp_001488"
     CDS             143..901
                     /gene="ydiY"
                     /locus_tag="ACHKYO_RS07440"
                     /old_locus_tag="pgaptmp_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HGU4428355.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(954..1061)
                     /gene="yniD"
                     /locus_tag="ACHKYO_RS07445"
                     /old_locus_tag="pgaptmp_001489"
     CDS             complement(954..1061)
                     /gene="yniD"
                     /locus_tag="ACHKYO_RS07445"
                     /old_locus_tag="pgaptmp_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HGU4428356.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1237..3136)
                     /gene="espL1"
                     /locus_tag="ACHKYO_RS07450"
                     /old_locus_tag="pgaptmp_001490"
                     /pseudo
     CDS             complement(1237..3136)
                     /gene="espL1"
                     /locus_tag="ACHKYO_RS07450"
                     /old_locus_tag="pgaptmp_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            3660..5588
                     /gene="thrS"
                     /locus_tag="ACHKYO_RS07455"
                     /old_locus_tag="pgaptmp_001491"
     CDS             3660..5588
                     /gene="thrS"
                     /locus_tag="ACHKYO_RS07455"
                     /old_locus_tag="pgaptmp_001491"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGU4428357.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            5592..6134
                     /gene="infC"
                     /locus_tag="ACHKYO_RS07460"
                     /old_locus_tag="pgaptmp_001492"
     CDS             5592..6134
                     /gene="infC"
                     /locus_tag="ACHKYO_RS07460"
                     /old_locus_tag="pgaptmp_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGU4428358.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            6231..6428
                     /gene="rpmI"
                     /locus_tag="ACHKYO_RS07465"
                     /old_locus_tag="pgaptmp_001493"
     CDS             6231..6428
                     /gene="rpmI"
                     /locus_tag="ACHKYO_RS07465"
                     /old_locus_tag="pgaptmp_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGU4428359.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            6481..6837
                     /gene="rplT"
                     /locus_tag="ACHKYO_RS07470"
                     /old_locus_tag="pgaptmp_001494"
     CDS             6481..6837
                     /gene="rplT"
                     /locus_tag="ACHKYO_RS07470"
                     /old_locus_tag="pgaptmp_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGU4428360.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    6954..7078
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6960..7004
                     /gene="pheM"
                     /locus_tag="ACHKYO_RS07475"
                     /old_locus_tag="pgaptmp_001495"
     CDS             6960..7004
                     /gene="pheM"
                     /locus_tag="ACHKYO_RS07475"
                     /old_locus_tag="pgaptmp_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HGU4428361.1"
                     /translation="MNAAIFRFFFYFST"
     gene            7288..8271
                     /gene="pheS"
                     /locus_tag="ACHKYO_RS07480"
                     /old_locus_tag="pgaptmp_001496"
     CDS             7288..8271
                     /gene="pheS"
                     /locus_tag="ACHKYO_RS07480"
                     /old_locus_tag="pgaptmp_001496"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGU4428362.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            8286..10673
                     /gene="pheT"
                     /locus_tag="ACHKYO_RS07485"
                     /old_locus_tag="pgaptmp_001497"
     CDS             8286..10673
                     /gene="pheT"
                     /locus_tag="ACHKYO_RS07485"
                     /old_locus_tag="pgaptmp_001497"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGU4428363.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVTVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            10678..10977
                     /gene="ihfA"
                     /locus_tag="ACHKYO_RS07490"
                     /old_locus_tag="pgaptmp_001498"
     CDS             10678..10977
                     /gene="ihfA"
                     /locus_tag="ACHKYO_RS07490"
                     /old_locus_tag="pgaptmp_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HGU4428364.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            11078..12058
                     /gene="btuC"
                     /locus_tag="ACHKYO_RS07495"
                     /old_locus_tag="pgaptmp_001499"
     CDS             11078..12058
                     /gene="btuC"
                     /locus_tag="ACHKYO_RS07495"
                     /old_locus_tag="pgaptmp_001499"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HGU4428365.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADVVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            12121..12672
                     /gene="btuE"
                     /locus_tag="ACHKYO_RS07500"
                     /old_locus_tag="pgaptmp_001500"
     CDS             12121..12672
                     /gene="btuE"
                     /locus_tag="ACHKYO_RS07500"
                     /old_locus_tag="pgaptmp_001500"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HGU4428366.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            12672..13421
                     /gene="btuD"
                     /locus_tag="ACHKYO_RS07505"
                     /old_locus_tag="pgaptmp_001501"
     CDS             12672..13421
                     /gene="btuD"
                     /locus_tag="ACHKYO_RS07505"
                     /old_locus_tag="pgaptmp_001501"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HGU4428367.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            13499..13963
                     /gene="nlpC"
                     /locus_tag="ACHKYO_RS07510"
                     /old_locus_tag="pgaptmp_001502"
     CDS             13499..13963
                     /gene="nlpC"
                     /locus_tag="ACHKYO_RS07510"
                     /old_locus_tag="pgaptmp_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU4428368.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            14210..14923
                     /gene="ydiV"
                     /locus_tag="ACHKYO_RS07515"
                     /old_locus_tag="pgaptmp_001503"
     CDS             14210..14923
                     /gene="ydiV"
                     /locus_tag="ACHKYO_RS07515"
                     /old_locus_tag="pgaptmp_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HGU4428369.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            14986..16422
                     /gene="selO"
                     /locus_tag="ACHKYO_RS07520"
                     /old_locus_tag="pgaptmp_001504"
     CDS             14986..16422
                     /gene="selO"
                     /locus_tag="ACHKYO_RS07520"
                     /old_locus_tag="pgaptmp_001504"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HGU4428370.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(16426..16617)
                     /gene="hemP"
                     /locus_tag="ACHKYO_RS07525"
                     /old_locus_tag="pgaptmp_001505"
     CDS             complement(16426..16617)
                     /gene="hemP"
                     /locus_tag="ACHKYO_RS07525"
                     /old_locus_tag="pgaptmp_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HGU4428371.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(16749..17795)
                     /gene="aroH"
                     /locus_tag="ACHKYO_RS07530"
                     /old_locus_tag="pgaptmp_001506"
     CDS             complement(16749..17795)
                     /gene="aroH"
                     /locus_tag="ACHKYO_RS07530"
                     /old_locus_tag="pgaptmp_001506"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HGU4428372.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHKFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(17952..18785)
                     /gene="ppsR"
                     /locus_tag="ACHKYO_RS07535"
                     /old_locus_tag="pgaptmp_001507"
     CDS             complement(17952..18785)
                     /gene="ppsR"
                     /locus_tag="ACHKYO_RS07535"
                     /old_locus_tag="pgaptmp_001507"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HGU4428373.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            19118..21496
                     /gene="ppsA"
                     /locus_tag="ACHKYO_RS07540"
                     /old_locus_tag="pgaptmp_001508"
     CDS             19118..21496
                     /gene="ppsA"
                     /locus_tag="ACHKYO_RS07540"
                     /old_locus_tag="pgaptmp_001508"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HGU4428374.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(21553..23199)
                     /gene="fadK"
                     /locus_tag="ACHKYO_RS07545"
                     /old_locus_tag="pgaptmp_001509"
     CDS             complement(21553..23199)
                     /gene="fadK"
                     /locus_tag="ACHKYO_RS07545"
                     /old_locus_tag="pgaptmp_001509"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HGU4428375.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYNYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(23256..23549)
                     /gene="ydiT"
                     /locus_tag="ACHKYO_RS07550"
                     /old_locus_tag="pgaptmp_001510"
     CDS             complement(23256..23549)
                     /gene="ydiT"
                     /locus_tag="ACHKYO_RS07550"
                     /old_locus_tag="pgaptmp_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU4428376.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(23546..24835)
                     /gene="ydiS"
                     /locus_tag="ACHKYO_RS07555"
                     /old_locus_tag="pgaptmp_001511"
     CDS             complement(23546..24835)
                     /gene="ydiS"
                     /locus_tag="ACHKYO_RS07555"
                     /old_locus_tag="pgaptmp_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4428377.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SGHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(24891..25829)
                     /gene="ydiR"
                     /locus_tag="ACHKYO_RS07560"
                     /old_locus_tag="pgaptmp_001512"
     CDS             complement(24891..25829)
                     /gene="ydiR"
                     /locus_tag="ACHKYO_RS07560"
                     /old_locus_tag="pgaptmp_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HGU4428378.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(25849..26613)
                     /gene="ydiQ"
                     /locus_tag="ACHKYO_RS07565"
                     /old_locus_tag="pgaptmp_001513"
     CDS             complement(25849..26613)
                     /gene="ydiQ"
                     /locus_tag="ACHKYO_RS07565"
                     /old_locus_tag="pgaptmp_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HGU4428379.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQAGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            26929..27840
                     /gene="ydiP"
                     /locus_tag="ACHKYO_RS07570"
                     /old_locus_tag="pgaptmp_001514"
     CDS             26929..27840
                     /gene="ydiP"
                     /locus_tag="ACHKYO_RS07570"
                     /old_locus_tag="pgaptmp_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4428380.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAISDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(27883..29034)
                     /gene="ydiO"
                     /locus_tag="ACHKYO_RS07575"
                     /old_locus_tag="pgaptmp_001515"
     CDS             complement(27883..29034)
                     /gene="ydiO"
                     /locus_tag="ACHKYO_RS07575"
                     /old_locus_tag="pgaptmp_001515"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HGU4428381.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(29048..30643)
                     /gene="ydiF"
                     /locus_tag="ACHKYO_RS07580"
                     /old_locus_tag="pgaptmp_001516"
     CDS             complement(29048..30643)
                     /gene="ydiF"
                     /locus_tag="ACHKYO_RS07580"
                     /old_locus_tag="pgaptmp_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HGU4428382.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(30787..31545)
                     /gene="aroD"
                     /locus_tag="ACHKYO_RS07585"
                     /old_locus_tag="pgaptmp_001517"
     CDS             complement(30787..31545)
                     /gene="aroD"
                     /locus_tag="ACHKYO_RS07585"
                     /old_locus_tag="pgaptmp_001517"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGU4428383.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(31576..32442)
                     /gene="ydiB"
                     /locus_tag="ACHKYO_RS07590"
                     /old_locus_tag="pgaptmp_001518"
     CDS             complement(31576..32442)
                     /gene="ydiB"
                     /locus_tag="ACHKYO_RS07590"
                     /old_locus_tag="pgaptmp_001518"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HGU4428384.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(32454..33719)
                     /gene="ydiN"
                     /locus_tag="ACHKYO_RS07595"
                     /old_locus_tag="pgaptmp_001519"
     CDS             complement(32454..33719)
                     /gene="ydiN"
                     /locus_tag="ACHKYO_RS07595"
                     /old_locus_tag="pgaptmp_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4428385.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(33946..35160)
                     /gene="ydiM"
                     /locus_tag="ACHKYO_RS07600"
                     /old_locus_tag="pgaptmp_001520"
     CDS             complement(33946..35160)
                     /gene="ydiM"
                     /locus_tag="ACHKYO_RS07600"
                     /old_locus_tag="pgaptmp_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4428386.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMLINALFLYRCTFPPHPGRRLPVIKKTTRSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLSQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(35260..35616)
                     /gene="ydiL"
                     /locus_tag="ACHKYO_RS07605"
                     /old_locus_tag="pgaptmp_001521"
     CDS             complement(35260..35616)
                     /gene="ydiL"
                     /locus_tag="ACHKYO_RS07605"
                     /old_locus_tag="pgaptmp_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HGU4428387.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(35752..35859)
                     /gene="rprA"
                     /locus_tag="ACHKYO_RS07610"
                     /old_locus_tag="pgaptmp_001522"
     ncRNA           complement(35752..35859)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKYO_RS07610"
                     /old_locus_tag="pgaptmp_001522"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(36045..37157)
                     /gene="ydiK"
                     /locus_tag="ACHKYO_RS07615"
                     /old_locus_tag="pgaptmp_001523"
     CDS             complement(36045..37157)
                     /gene="ydiK"
                     /locus_tag="ACHKYO_RS07615"
                     /old_locus_tag="pgaptmp_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HGU4428388.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            37546..40602
                     /gene="ydiJ"
                     /locus_tag="ACHKYO_RS07620"
                     /old_locus_tag="pgaptmp_001524"
     CDS             37546..40602
                     /gene="ydiJ"
                     /locus_tag="ACHKYO_RS07620"
                     /old_locus_tag="pgaptmp_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HGU4428389.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            40599..41009
                     /gene="menI"
                     /locus_tag="ACHKYO_RS07625"
                     /old_locus_tag="pgaptmp_001525"
     CDS             40599..41009
                     /gene="menI"
                     /locus_tag="ACHKYO_RS07625"
                     /old_locus_tag="pgaptmp_001525"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HGU4428390.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            41109..41297
                     /gene="ydiH"
                     /locus_tag="ACHKYO_RS07630"
                     /old_locus_tag="pgaptmp_001526"
     CDS             41109..41297
                     /gene="ydiH"
                     /locus_tag="ACHKYO_RS07630"
                     /old_locus_tag="pgaptmp_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HGU4428391.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            41845..42213
                     /gene="sufA"
                     /locus_tag="ACHKYO_RS07635"
                     /old_locus_tag="pgaptmp_001527"
     CDS             41845..42213
                     /gene="sufA"
                     /locus_tag="ACHKYO_RS07635"
                     /old_locus_tag="pgaptmp_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HGU4428392.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            42222..43709
                     /gene="sufB"
                     /locus_tag="ACHKYO_RS07640"
                     /old_locus_tag="pgaptmp_001528"
     CDS             42222..43709
                     /gene="sufB"
                     /locus_tag="ACHKYO_RS07640"
                     /old_locus_tag="pgaptmp_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGU4428393.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            43719..44465
                     /gene="sufC"
                     /locus_tag="ACHKYO_RS07645"
                     /old_locus_tag="pgaptmp_001529"
                     /pseudo
     CDS             43719..44465
                     /gene="sufC"
                     /locus_tag="ACHKYO_RS07645"
                     /old_locus_tag="pgaptmp_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
     gene            44440..45711
                     /gene="sufD"
                     /locus_tag="ACHKYO_RS07650"
                     /old_locus_tag="pgaptmp_001530"
     CDS             44440..45711
                     /gene="sufD"
                     /locus_tag="ACHKYO_RS07650"
                     /old_locus_tag="pgaptmp_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGU4428394.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            45708..46928
                     /gene="sufS"
                     /locus_tag="ACHKYO_RS07655"
                     /old_locus_tag="pgaptmp_001531"
     CDS             45708..46928
                     /gene="sufS"
                     /locus_tag="ACHKYO_RS07655"
                     /old_locus_tag="pgaptmp_001531"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HGU4428395.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            46941..47357
                     /gene="sufE"
                     /locus_tag="ACHKYO_RS07660"
                     /old_locus_tag="pgaptmp_001532"
     CDS             46941..47357
                     /gene="sufE"
                     /locus_tag="ACHKYO_RS07660"
                     /old_locus_tag="pgaptmp_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HGU4428396.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            47506..48510
                     /gene="ldtE"
                     /locus_tag="ACHKYO_RS07665"
                     /old_locus_tag="pgaptmp_001533"
     CDS             47506..48510
                     /gene="ldtE"
                     /locus_tag="ACHKYO_RS07665"
                     /old_locus_tag="pgaptmp_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HGU4428397.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(48574..48810)
                     /gene="lpp"
                     /locus_tag="ACHKYO_RS07670"
                     /old_locus_tag="pgaptmp_001534"
     CDS             complement(48574..48810)
                     /gene="lpp"
                     /locus_tag="ACHKYO_RS07670"
                     /old_locus_tag="pgaptmp_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HGU4428398.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(49121..50533)
                     /gene="pykF"
                     /locus_tag="ACHKYO_RS07675"
                     /old_locus_tag="pgaptmp_001535"
     CDS             complement(49121..50533)
                     /gene="pykF"
                     /locus_tag="ACHKYO_RS07675"
                     /old_locus_tag="pgaptmp_001535"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HGU4428399.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(50541..50735)
                     /locus_tag="ACHKYO_RS07680"
                     /old_locus_tag="pgaptmp_001536"
     CDS             complement(50541..50735)
                     /locus_tag="ACHKYO_RS07680"
                     /old_locus_tag="pgaptmp_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4428400.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            51090..51299
                     /gene="fumD"
                     /locus_tag="ACHKYO_RS07685"
                     /old_locus_tag="pgaptmp_001537"
     CDS             51090..51299
                     /gene="fumD"
                     /locus_tag="ACHKYO_RS07685"
                     /old_locus_tag="pgaptmp_001537"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HGU4428401.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT        13152 a        12274 c        13120 g        12927 t
ORIGIN      
        1 aaaaaatgaa ataagaatac attgctaagt tattcgcgcc tttttataat tcgttacctt
       61 tccagggacg ctaatctttg atccttaaga aagtttccgg gaccgcgatg gtctcttttt
      121 ttgttgtacg gactcttgat aaatgaagct tttgaagaca gttcctgcca tcgttatgtt
      181 ggcggggggc atgtttgcgt cactgaatgc agctgccgat gattccgttt ttactgtcat
      241 ggacgatcct gccagcgcta aaaaaccttt cgaaggtaat ctgaacgcag gatatcttgc
      301 acaatcaggc aatacgaaaa gctcctcact tacggctgac accaccatga cctggtatgg
      361 acaaaccact gcctggtcgc tgtgggggaa cgccagtaat acttcttcta atgatgagcg
      421 ttcttcagaa aaatatgcgg cgggcgggcg tagccgtttt aatttaactg attatgacta
      481 tttatttggt caggcaagct ggctgacaga ccgttataac ggctatcgag agcgcgatgt
      541 gttgaccgcg ggttatggtc gtcagttttt gaatggcccg gtacacagtt tccgctttga
      601 attcggtcca ggtgtgcgtt acgacaaata taccgacaat gccagtgaaa ctcagccgct
      661 gggctatgcc tccggtgctt atgcatggca gttgactgac aacgctaaat ttacgcaggg
      721 tgtttccgtc tttggcgcgg aagacacaac gctgaactcc gaaagcgcat taaatgttgc
      781 tattaatgaa cattttggac tgaaagtggc ttacaacgtc acatggaact ctgaacctcc
      841 agagtctgca ccggaacata ccgatcgtcg gacaacctta tcgctgggct acagcatgta
      901 aaattatctg ccggaccgtg ttatgcggtc tggctgtctt cgtttgtcac tgctcagccc
      961 caaatcattg ctgcccaacg tagcaacaac atagcgccac agattgccag tagcaccacc
     1021 accatcttcc agtgtttttt atcaaagcgt ttagtcggca taccagtgtc ctgcatatct
     1081 cttgtgcgtg ataacggagt gtaacaaaag ggacaggcat caagggattt tttgttatta
     1141 cagccgtcgg gtggcgttgt acacctgatc actgtttctg cgttttatga aaaaaataaa
     1201 attatgtatc gatatacaaa tatcataaat agctggttat aaatacgttt ttagttcatc
     1261 agcactaaaa ctcaggcttt ggctgattgc attaatagca gcataatatg tttctacagt
     1321 tttataatga ttatgatgaa tggctatatg aacagctgac atgttttcat gatttttagc
     1381 ggccaacagt gtgaataatt gacgttgact aaaagaatat ttttttgcaa aagtactcag
     1441 tgttgatata taagataaca cgacgtcttt attacccttg ctcatggcga tgtataaagc
     1501 aggggttcca tgtgctgtcg cgccttttaa taagtccatg atgttgattt tgctgagttt
     1561 atatttaacg gcgatcccat aaactttaga gaggaaccgt gtgacacaca aagggtgatt
     1621 attttccatt gcagcaaata accctggttc accatcacgt gttttcatgg ctaaaatatc
     1681 catcaccatt ttttttgttt aaaagttctt gtgctattgg agtggttaat gcattcagaa
     1741 agatatcgat tatttcatcg aatccataca acatggagat atagagtcca ggtgtttcgt
     1801 tagcactttt agtttgcagt aatttaacca gattatcttc atgaatgata tgattgtata
     1861 ccagtgactg gatttcttgc ataaatattt ttacgttgtc agtttgtccg ttcatcatag
     1921 ccagaaataa agcaggtgta ccaacgctat tctgcgcagt tattatttca atgatttctt
     1981 tttcagggag tatgggcaac tgtttgaatc gtgtaataag ctattgagtc tggcctgtgg
     2041 ccattattaa caacattgcc gatggatgta taggggcaaa tggcattcgt gtcgtgatac
     2101 ccgctaaaat agcaactgga actccctcag gtaatagagg aagaatagca attgcacagt
     2161 tgcgaacata cctatcagcc caggtctgag catcgggagc tgaactgatg aacttatcaa
     2221 gcgagagaaa gcgccgtgct gtcctgtggg tgcctctgac ggcaacgttg gtatcgttcg
     2281 ggtcgtaaac agacacaaca taatttaatt gtccatacac tgactgcttt attcgtagcc
     2341 ttactgacaa agtatgattg tctatatata aaatcgcgga catatgactg acattgtttg
     2401 ccatcattcg acaaaagaaa atatctaaag cgaagccaaa atccttactc gcgataacgc
     2461 gactccctgc aggaggatgg cgaagtaatg ctctgaactc cgcatcccgg actaagggga
     2521 tatgctgctg tatggattca acggaggagt aatgtttaat caggttgact ttgccttttc
     2581 catttctcaa tgaatcaagg gcgtattgca atgacagatg gcgacaaaaa atagcgtcag
     2641 aaggagattg caaaaaacat gcactaccat tgagatttac tgttgggtgt atagtttttt
     2701 ctctatggat ttttttagac agtttcatac aatggttacg atcttcagca gttaactgtt
     2761 cctgttcaat ataatccagt atgcttcgca aatggttagc tttaataggc gtggtttcca
     2821 gtaataagga aatgaaatct tttttatagt gtaatgtaat gagtatattt tctgcatgga
     2881 tgggtgaagt ggcaaagtta atggtaaaaa gggcagtgga ataacaattt tgtgatattt
     2941 ttcgacagtt tgcggtaaat tcctgaataa cacagtatga cagcatcgaa cttaagtcag
     3001 aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct gatgaaggtg
     3061 ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt ctgataatga
     3121 tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg aggatgatga
     3181 ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt
     3241 ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc
     3301 aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa
     3361 acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct ggtgtactca
     3421 ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct cccttttttt
     3481 agtgttaaca tcgctcaacc gggtggaagg tgagaggtta atgtctttca tttgtgtacc
     3541 tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac tgttaataaa
     3601 caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa ggatataaaa
     3661 tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct gtaagcccca
     3721 tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta
     3781 atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg tccatcatta
     3841 ccgccaaaga cgaagacggt ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc
     3901 acgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt attgacaacg
     3961 gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc gaagcactcg
     4021 agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag aaagtcagct
     4081 ggcacgaagc gcgtgaaaca ttcgccaacc gtggggagag ctacaaagtc tccattcttg
     4141 acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa tatgtcgata
     4201 tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa
     4261 cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt atttacggta
     4321 cggcgtgggc agacaaaaaa gcgcttaacg cttacctgca gcgcctggaa gaagccgcga
     4381 aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc
     4441 cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg gaagtgtttg
     4501 ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc atgatggacc
     4561 gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg ttcaccacgt
     4621 cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac gtacaaattt
     4681 tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag tttggtagct
     4741 gccaccgtaa tgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc
     4801 aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt aacggatgta
     4861 tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc gtcaaactct
     4921 ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct gaggcggacc
     4981 tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt gaaggcgctt
     5041 tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca tggcagtgcg
     5101 gtacagtaca gctggacttc tctttgcctt ctcgtctgag cgcttcttat gtaggcgaag
     5161 acaatgaacg taaagtaccg gtaatgattc accgcgcaat tctggggtcg atggaacgtt
     5221 tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt gcgccagttc
     5281 aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa ttgacgcaaa
     5341 aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag attggcttta
     5401 aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt gataaagagg
     5461 tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga agcatggacg
     5521 taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt aaacaattgg
     5581 aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg
     5641 gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag cttggtattg
     5701 tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta gtcgagatca
     5761 gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc ctctatgaaa
     5821 agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta
     5881 aattccgtcc tggtacagat gaaggcgact atcaggtaaa actccgcagc ctgattcgct
     5941 ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc
     6001 accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag
     6061 tggtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg ctcgctccta
     6121 agaagaaaca gtaaggcctt caagtagcac tgtctgtgga gccttcgggc ttcacaggct
     6181 ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa atgccaaaaa
     6241 ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc
     6301 acaagcacgc taacctgcgt cacattctga ccaaaaaagc gaccaaacgt aaacgtcacc
     6361 tgcgtccaaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg tgcctgccgt
     6421 acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag gagagcacat
     6481 atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa
     6541 gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca ggctgttatc
     6601 aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg
     6661 tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag caaattcatc
     6721 aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga tatcgcagta
     6781 ttcgacaaag tagcgttcac cgctctggtt gaaaaagcga aagcagctct ggcataagcc
     6841 agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa cattgacttt
     6901 tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa
     6961 tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag gaggctagcg
     7021 cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct ttttttgtat
     7081 gcgcgttttg aaaaataaaa agcctgtctc atcaggtgta attgtcgcag ccagtctgga
     7141 cacggccagc gcggagaaac cggagcgtat ttaagtacgt gagaatttcg agcactgccc
     7201 gggaccaaaa tggcaagtaa aatagcctga tgggataggc tctaagtcca acgaaccagt
     7261 gtcaccactg acacaatgag gaaaaccatg tcacatctcg cagaactggt tgccagtgcg
     7321 aaggcggcca ttagccaggc gtcagatgtt gccgcgttag ataatgtgcg cgtcgaatat
     7381 ttgggtaaaa aagggcactt aacccttcag atgacgaccc tgcgtgagct gccgccagaa
     7441 gagcgtccgg cagctggtgc ggttatcaac gaagcgaaag agcaggttca gcaggcgctg
     7501 aatgcgcgta aagcggaact ggaaagcgct gcactgaatg cgcgtctggc ggcggaaacg
     7561 attgatgtct ccctgccagg tcgtcgcatt gaaaacggcg gtctgcatcc ggttacccgt
     7621 accatcgacc gtatcgaaag tttcttcggt gagcttggct ttaccgtggc aaccgggccg
     7681 gaaatcgaag acgattatca taacttcgat gctctgaaca ttcctggtca ccacccggcg
     7741 cgcgctgacc acgacacttt ctggtttgac actacccgcc tgctgcgtac ccagacctct
     7801 ggcgtacaga tccgcaccat gaaagcccag cagccaccga ttcgtatcat cgcgcctggc
     7861 cgcgtttatc gtaacgacta cgaccagact cacacgccga tgttccatca gatggaaggt
     7921 ctgattgttg ataccaacat cagctttacc aacctgaaag gcactctgca cgatttcctg
     7981 cgtaacttct ttgaggaaga tttgcagatt cgcttccgtc cttcctactt cccgttcacc
     8041 gagccttctg cagaagttga cgtcatgggt aaaaacggta aatggctgga agtgctgggt
     8101 tgcgggatgg tgcatccgaa cgtgctgcgt aacgttggca tcgacccgga agtttactct
     8161 ggtttcgcct tcgggatggg gatggagcgt ctgactatgt tgcgttacgg cgtcaccgac
     8221 ctgcgttcat tcttcgaaaa cgatctgcgt ttcctcaaac agtttaaata aggcaggaat
     8281 agattatgaa attcagtgaa ctgtggttac gcgaatgggt gaacccggcg attgatagcg
     8341 atgcgctggc gaatcaaatc actatggcgg gcctggaagt tgacggtgta gaaccggttg
     8401 ccggtagctt ccacggcgtg gtcgttggtg aagtggttga gtgtgcgcag catccgaacg
     8461 ctgacaaact gcgtgtgaca aaagtgaatg ttggcggcga tcgcctgctg gacatcgtct
     8521 gcggtgcgcc aaactgccgt cagggcctgc gtgtggcggt agctaccatt ggtgctgttc
     8581 tgccgggtga tttcaaaatt aaagcggcga aactgcgtgg tgaaccgtct gaagggatgc
     8641 tgtgctcctt ttctgagctg ggaatttctg acgatcatag cggcattatc gagctgcctg
     8701 cggatgcgcc gattggcacc gacatccgtg aatacctgaa acttgatgac aacaccatcg
     8761 aaatcagcgt gacgccaaac cgtgccgact gtttaggtat cattggtgtt gcgcgtgacg
     8821 ttaccgtgct gaatcagctg ccgctggttg aaccggaaat cgttccggtt ggtgcgacca
     8881 tcgacgacac gctgccgatt acagtcgaag cgccggaagc ctgcccgcgt tatcttggcc
     8941 gtgtggtaaa aggcattaac gttaaagcac caactccgct gtggatgaaa gaaaaactgc
     9001 gtcgttgcgg gatccgttct atcgatgcag ttgttgacgt caccaactat gtactgctcg
     9061 aactgggcca gccgatgcac gctttcgata aagatcgcat tgaaggcggc attgtggtgc
     9121 ggatggcgaa agagggcgaa acgttggtac tgcttgatgg cactgaagcg aagctgaatg
     9181 ctgacactct ggtcatcgcc gaccacaaca aggcgctggc gatgggcggc atctttggtg
     9241 gcgaacactc tggcgtgaat gacgaaacgc aaaacgtctt gctggaatgc gctttcttca
     9301 gcccgctgtc tatcaccggt cgtgctcgtc gtcatggcct gcatactgat gcgtctcacc
     9361 gttatgagcg tggcgttgat ccggcactgc agcacaaagc gatggaacgt gcgacccgtc
     9421 tactgatcga catctgcggt ggtgaggctg gtccggtaat tgatatcacc aacgaagcaa
     9481 cgctgccgaa gcgtgcaacc atcactttac gtcgtagcaa actggatcgc ctgatcggcc
     9541 atcatattgc ggatgagcag gtaactgaca ttctgcgtcg tctcggctgc gaagtgaccg
     9601 aaggcaaaga cgagtggcag gcagttgcgc cgagctggcg tttcgatatg gagattgaag
     9661 aagatctggt cgaagaagtc gcgcgtgttt acggctacaa caacatcccg gatgagccgg
     9721 tacaggcaag cctgattatg ggtactcacc gtgaagctga cctgtcgctc aagcgcgtga
     9781 aaacgctgct caacgacaaa ggctatcagg aagtgatcac ctacagcttc gttgatccga
     9841 aagtgcagca gatgatccat ccaggcgttg aagccttact gctgccaagc ccgatctctg
     9901 ttgaaatgtc agcaatgcgt ctttctctgt ggaccggcct gctggcaacc gtggtgtaca
     9961 accagaaccg tcagcagaac cgtgtgcgca ttttcgaaag cggtctgcgt ttcgtaccag
    10021 atactcaggc accgttgggc attcgtcagg atctgatgtt agccggtgtg atttgcggta
    10081 accgttacga agagcactgg aacctggcaa aagagaccgt tgatttctat gatttgaaag
    10141 gcgatcttga atccgttctc gacctgaccg gtaaactgaa tgaggttgag ttccgtgcag
    10201 aagcgaatcc ggcactgcat ccggggcaat ccgcagcgat ttatctgaaa ggtgaacgta
    10261 ttggttttgt tggggttgtt catcctgaac tggaacgtaa actggatctt aacggtcgca
    10321 ctctggtgtt cgaactggag tggaacaagc tcgcagaccg cgtggtgcct caggcgcgcg
    10381 agatttctcg cttcccggcg aaccgtcgtg acatcgcggt ggtggtcgca gaaaacgttc
    10441 ccgcagcgga tattttatcc gaatgtaaga aagttggcgt aaatcaggta gttggcgtaa
    10501 acttatttga cgtgtaccgc ggtaagggtg ttgcggaggg gtataagagc ctcgccataa
    10561 gcctgatcct gcaagatacc agccgtacac tcgaagaaga ggagattgcc gctaccgtcg
    10621 ccaaatgtgt agaggcatta aaagagcgat tccaggcatc attgagggat tgaacctatg
    10681 gcgcttacaa aagctgaaat gtcagaatat ctgtttgata agcttgggct tagcaagcgg
    10741 gatgccaaag aactggttga actgtttttc gaagagatcc gtcgcgctct ggaaaacggc
    10801 gaacaggtga aactctctgg ttttggtaac ttcgatctgc gtgataagaa tcaacgcccg
    10861 ggacgtaacc cgaaaacggg cgaggatatt cccattacag cacggcgcgt ggtgaccttc
    10921 agacccgggc agaagttaaa aagccgggtc gaaaacgctt cgcccaaaga cgagtaatct
    10981 gatctaacta aaaaggccgc tctgcggcct tttttctttt cactgtcgaa gagtcaccgt
    11041 aaaatcaacg ccatgacact tcagcagaac ggataccatg ctgacacttg cccgccaaca
    11101 acagcgacaa aatattcgct ggttattatg cctgtcagtt ttgatgctgc tggcgcttct
    11161 cttaagcctt tgcgccggtg aacaatggat ctcgccaggt gactggtttt ctcctcgtgg
    11221 cgaactgttc gtctggcaga ttcgcctgcc acgtacgctg gctgtattgc tggttggtgc
    11281 ggcgctggct atatccggcg cagtaatgca ggcgttgttt gaaaatcctc tggcagaacc
    11341 tggactactt ggcgtctcta acggcgcagg cgtagggctt atcgccgcgg tattgcttgg
    11401 gcaagggcaa ctcccaaact gggcactagg gctgtgtgcg attgctggcg cgcttatcat
    11461 cactttaata ctcttacgtt tcgcccgtcg tcatctttcg accagtcggt tattgctggc
    11521 tggcgttgca ttagggatta tctgtagcgc actaatgacg tgggctatct acttttccac
    11581 ctctgttgat ttacgtcagc tgatgtactg gatgatgggc ggttttggcg gcgtagactg
    11641 gcggcaaagc tggctgatgc tggcattgat ccccgtgttg ttgtggatct gttgtcagtc
    11701 caggccgatg aatatgttag cacttggcga gatctcggcg cggcaactgg gtttacccct
    11761 gtggttctgg cgcaatgtgc tggtggcagc gaccggctgg atggttggcg tcagtgtggc
    11821 gctggcgggt gctatcggct ttattggtct ggtgatccca catattcttc ggttgtgtgg
    11881 tttaaccgat catcgcgtat tacttcccgg ctgcgcgctg gcaggggcga gcgcattgct
    11941 gctggccgat gttgtagcgc gcttggcatt agctgccgca gagctgccta ttggcgtggt
    12001 caccgcaacg ttgggtgcgc cggtgtttat ctggttattg ttaaaagcag gacgttagcc
    12061 gcaaaaagac ggtctatgat taaaagctaa gatttttact gaccacaccc aggagaaacg
    12121 atgcaagatt ccattctgac gaccgtagtg aaagatatcg acggtgaagt gaccacgctg
    12181 gagaagtacg ccggtaatgt gctgttgatt gtcaatgtcg cctcaaagtg tggcttaacg
    12241 ccgcaatatg agcagttgga gaatattcag aaagcctggg ccgatcgagg ttttgtggtg
    12301 ctgggattcc cgtgcaacca gtttctggaa caagaaccgg gcagcgatga agagattaaa
    12361 acttactgta ccaccacatg gggggtgacg ttcccgatgt tcagtaagat tgaagttaat
    12421 ggcgaaggac gccatccgct gtatcaaaaa ttgattgccg cagcgccgac cgcagtcgcg
    12481 ccggaagaga gcggattcta tgcccgtatg gtcagcaaag gtcgtgcacc gctgtaccct
    12541 gatgatattt tatggaattt tgagaaattt ttagttggca gggacggatt ggtcatccag
    12601 cgtttttccc cggatatgac gccggaagac cccattgtga tggaaagcat taaactggcg
    12661 ttggcaaaat aatgtctatt gtgatgcagt tacaggatgt tgcggaatct acccgcctgg
    12721 ggccactttc tggcgaggtt cgggctgggg agatcctgca cctggtgggg ccgaatggcg
    12781 cgggtaagag taccttgctt gcacgaatgg ccggaatgac cagcggtaag ggaagcattc
    12841 agttcgcggg gcaacccctg gaagcatggt ccgcaacaaa actcgcgctg catcgcgcct
    12901 atctttcaca acagcagacg ccgccgtttg caatgccggt ctggcactac ctgacactgc
    12961 atcagcacga taaaacgcgt accgaactac tgaatgatgt cgcaggggcg ctggctcttg
    13021 atgacaaact cggtcgtagc accaatcaac tttccggcgg tgaatggcaa cgcgtacgtc
    13081 ttgctgcggt ggtgctgcaa atcacaccac aagccaatcc cgcaggtcaa ttgctgcttc
    13141 ttgatgagcc gatgaacagt cttgatgttg cgcaacaaag tgcgttagac aaaattctga
    13201 gcgctctgtg tcagcaagga ctggcgattg tgatgagcag tcacgatctc aaccacacat
    13261 tgcgtcatgc gcatcgggcg tggttgctaa aaggtggaaa aatgctggcc agtggacgca
    13321 gagaagaggt gctcacgccg ccaaatctgg cgcaggccta tgggatgaat tttcgccgtc
    13381 tggatatcga aggtcacaga atgctgattt cgaccatctg atttcattag tatcttgaaa
    13441 atcgggtaat tacgacgcta aattaatgca agaataaaaa acagaggaac cgtacggaat
    13501 gcgtttctgc cttattttga tcacagcact gtttctggcc gggtgtagcc accataaagc
    13561 accgccgcca aatgccagac tttctgattc gattaccgtt attgccggtt tgaacgacca
    13621 gctacaaagc tggcatggca cgccgtatcg ttatggtggc atgacgcggc gcggtgtgga
    13681 ctgttcggga tttgtggttg taacgatgcg cgatcgtttc gatttgcagc tgccccgaga
    13741 aaccaaagaa caagcctcta tcggcacgca aattgataaa gacgagttgc tgcctggtga
    13801 cctggtcttt ttcaaaacgg gttccggaca aaatggtttg catgtaggta tttacgatac
    13861 caacaaccaa tttatccacg cctctaccag caagggagtg atgcgttcct cacttgataa
    13921 tgtctattgg cagaaaaatt tctggcaggc gagacgaatt tagtatgtcg aaaaatggac
    13981 ggcgatgata catcgccgtc catttactcc tgaatacagt agtattttgc agacaaaaat
    14041 tccgtaaatg gattattcgt ttataatcag gataatctaa aaaaaggacc cctgaaaaag
    14101 tggttaacat ggaacaaatg aaagttactc cttatgtaat tgttatgtct tatggcctca
    14161 tcccttttag ccggatactg aaaaacatcc ttcgagaggg acggttacca tgaagatttt
    14221 tttggagaat ctttatcatt ctgattgcta tttcctgccg ataagagata accagcaaga
    14281 ccttgtcggc gttgagttaa ttacccattt ctccagtgaa gatggtacgg ttcgtatccc
    14341 gacgagtcgg gttatagcgc aacttaccga ggagcaacat tggcaattat tttcagagca
    14401 gttggaatta ctaaaatcat gccaacattt ttttattcaa cataaactct ttgcctggct
    14461 taatttaacg ccacaagtgg ctacattatt attagagagg gataattacg caggcgaact
    14521 attaaaatat ccgtttattg aactgttgat taatgagaat tacccgcatc tcaatgaagg
    14581 aaaagataat cgcgatcttt tatccttatc gcaaatgtat ccccttgtgt taggcaattt
    14641 gggagcaggt aatagcacaa tgaaagcggt ttttgatggt cttttcaccc gcgtcatgct
    14701 ggataaaagt tttattcagc aacaaatcac gcatcgctcc ttcgaaccgt ttattcgtgc
    14761 gatccaggcg caaatttccc cttgttgcaa ttgcatcatc gcggggggca ttgatacgac
    14821 agaaatattg gcacagatta cccccttcga tttccatgct ctccagggat gcctgtggcc
    14881 tgccgttcct ataaatcaga taacgacgtt ggttcagcga taaccctcct gtttgctggt
    14941 gtttaagacg agagtaaccg tctacactat caaacaggag gatctatgac cctgtctttt
    15001 attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc tacgccttta
    15061 aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag tattccatcg
    15121 tcgctgttta aaaatgctgc aggtgtctgg ggcggcgaaa ccctactgcc tggtatgtca
    15181 ccactggcgc aggtttacag tggtcatcag ttcggcgtct gggcgggcca actgggtgat
    15241 gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat ggactggcat
    15301 ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc ggttttacgt
    15361 tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat tccgacgacc
    15421 cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt agagccaggt
    15481 gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga acatttttac
    15541 tatcgccgcg agccggaaaa ggttcgtcag ttagctgact ttgccattcg tcattactgg
    15601 tcacatcttg aagatgatga ggacaaatac cgtctctggt ttaacgatgt tgtcgcacgt
    15661 accgcatcgt taattgccca atggcagacg gtcggctttg ctcatggggt gatgaatacc
    15721 gacaacatgt cgctgctggg gctgacgctt gattacgggc cgtttggttt tcttgatgat
    15781 tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttacag ctttgataat
    15841 caacctgccg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc accatttgtt
    15901 gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt gacgcattat
    15961 ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga taacgcgcta
    16021 ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg cacattccgc
    16081 atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgatga gtttattgat
    16141 cgtgcggcat ttgatgactg gtttgcccgt tatcggaggc gtttgcaaca agacgaggtt
    16201 agcgatattg agcgtcagca actgatgcaa agcgttaacc cagctctggt gttgcgcaac
    16261 tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgacgga attgcaccgc
    16321 ctgcatgagg cgttgcgaaa tcctttcagc gacagagctg atgactatgt cagtcgtcca
    16381 cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact tggtcaacag
    16441 cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat cgataatcag
    16501 tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct gcggggaagc
    16561 ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat aacgcataaa
    16621 aattaataat aattgatata acaatgattc ttattatcaa tattatggca ggccagagca
    16681 agcagtaatg tgttgaaaag acaacaagtt gcgcagaaat gcccatccca tcaggaatgg
    16741 gcacgcattc agaagcgggt atctaccgca gaggcgagtt tttcgaccag gcgttcggta
    16801 tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg ctgaccgccg
    16861 acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc aatcgccgta
    16921 gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg acggtgctgc
    16981 ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc aaacttgtgc
    17041 agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt gccgccacgc
    17101 ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg accatttttg
    17161 tctggcgaga gaaacatatg gctggcgcgg gctgcgcgga tagcatccac ggcaatccgc
    17221 gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgccga agccatttca
    17281 cggtggatct gactttcggt agtacgtgcg ccaatcgcgc cccaactgat taaatcagca
    17341 ataaactgac cggtcaccat atcgaggaac tcagtcgcgg tagggacacc cagctcattt
    17401 acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata gctgccgttt
    17461 aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt ttcaaaatag
    17521 gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg cagacgggtg
    17581 gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat aaccaacagt
    17641 cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt gacatgggtg
    17701 gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac caggctctca
    17761 atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa tttgttgctt
    17821 ctacggcagg actaccggga agtgatgcgc atcacgataa accaatcgca gctgatttca
    17881 agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa ataagctaat
    17941 gcactagttc tctagtacat tcggcggcta agccccatga tatcgaggat cttggtggca
    18001 atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt acggtacaac
    18061 gcttctactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg gctgttctcc
    18121 cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc gaacaattta
    18181 tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc ggcaataaag
    18241 gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt gggggtttta
    18301 ccacagcgag aaacgccgag caggatcacc tgagcctggt ccagattgcg caacgaaata
    18361 ccgtcatcgt gggcaagggt gtaatcaatc gccgcaatgc gcgcatcata tttattgaga
    18421 ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag tttcatctct
    18481 tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc actttgcaag
    18541 atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg cacacctgtc
    18601 tggtgataaa tggcgtcaat ctgatccttc actgcccgtg cacggctctc attttcgaca
    18661 aacggcagcg taatgctgct gatagtgacg ggaaattgtg acattactgc gtgtcctaat
    18721 acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc aacagcatta
    18781 tccattttgt tcttcccgtg atgcagacat aattctatga aagcataaat taaaaacgca
    18841 cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcaatttta ttttttatga
    18901 aaacagtatt tcatttttat ggtttcgttt ataccgatgg tttatgtgga aattgtcgaa
    18961 gagagcagat ttgcgcaacg ctgggatcag tcttaaaaag taaaaaaata tatttgcttg
    19021 aacgattcac cgtttttttc atccggttaa atatgcaaag ataaatgcgc agaaatgtgt
    19081 ttctcaaacc gttcatttat cacaaaagga ttgttcgatg tccaacaatg gctcgtcacc
    19141 gctggtgctt tggtataacc aactcggcat gaatgatgta gacagggttg ggggcaaaaa
    19201 tgcctccctg ggtgaaatga ttactaacct ttccggaatg ggtgtttccg ttccgaatgg
    19261 tttcgccaca accgccgacg cgtttaacca gtttctggac caaagcggcg taaaccagcg
    19321 catttatgaa ctgctggata aaacggatat tgacgatgtt actcagcttg cgaaagcggg
    19381 cgcgcaaatc cgccagtgga ttatcgacac tcccttccag cctgagctgg aaaacgccat
    19441 ccgcgaagcc tatgcacagc tttccgccga tgacgaaaac gcctcgtttg cggtgcgttc
    19501 ctccgctact gcagaagata tgccggacgc ttcttttgcc ggtcagcagg aaaccttcct
    19561 caacgttcag ggttttgacg ccgttctcgt ggcagtgaaa catgtatttg cttctctgtt
    19621 taacgatcgc gccatctctt atcgtgtgca ccagggttac gatcaccgtg gcgtggcgct
    19681 ctccgccggt gttcagcgga tggtgcgctc cgacctcgca tcttctggcg tgatgttctc
    19741 cattgatacc gaatccggct ttgaccaggt ggtgtttatc acttccgcat ggggccttgg
    19801 ggaaatggtc gtgcagggtg cggttaaccc ggatgagttt tacgtccata aaccgacact
    19861 ggcggcgaat cgcccggcta tcgtgcgccg caccatgggg tcgaaaaaaa tccgcatggt
    19921 ttatgcgccg acccaggagc acggcaagca ggttaaaatc gaagacgtac cgcaggaaca
    19981 gcgtgacatc ttctcgctga ccaacgaaga agtgcaggaa ctggcaaaac aggccgtaca
    20041 aattgagaaa cactacggtc gcccaatgga tattgagtgg gcgaaagatg gccacaccgg
    20101 caaactgttc attgtgcagg cgcgtccgga aaccgtgcgc tcacgcggtc aggtcatgga
    20161 gcgttatacg ctgcattcac agggtaagat tatcgccgaa ggccgtgcta tcggtcatcg
    20221 catcggtgcg ggtccggtga aagtcatcca tgacatcagc gaaatgaacc gcatcgaacc
    20281 tggtgacgtg ctggtcactg atatgaccga cccggactgg gaaccgatca tgaagaaagc
    20341 atctgccatc gtcaccaacc gtggcggtcg tacctgtcac gcggcgatca tcgctcgtga
    20401 actgggcatt ccggcggtag tgggctgtgg agatgcaaca gaacggatga aagacggtga
    20461 gaacgtcact gtttcttgtg ccgaaggtga taccggttac gtctatgcgg agttgctgga
    20521 atttagcgtg aaaagctcca gcgtagaaac gatgccggat ctgccgttga aagtgatgat
    20581 gaacgtcggt aacccggacc gtgctttcga cttcgcctgt ctgccgaacg aaggcgtggg
    20641 ccttgcgcgt ctggaattta tcatcaaccg tatgattggc gtccacccac gcgcactgct
    20701 tgagtttgac gatcaggaac cgcagttgca aaacgaaatc cgcgagatga tgaaaggttt
    20761 tgattctccg cgtgaatttt acgttggtcg tctgactgaa gggatcgcga cgctgggtgc
    20821 cgcgttttat ccgaagcgcg tcattgtccg tctctctgat tttaaatcga acgaatatgc
    20881 caacctggtc ggtggtgagc gttacgagcc agatgaagag aacccgatgc tcggcttccg
    20941 tggcgcgggc cgctatgttt ccgacagctt ccgcgactgt ttcgcgctgg agtgtgaagc
    21001 agtgaaacgt gtgcgcaatg acatggggct gaccaacgtt gagatcatga tcccgttcgt
    21061 gcgtaccgta gatcaggcga aagcggtggt tgaagaactg gcgcgtcagg ggctgaaacg
    21121 tggcgagaac gggctgaaaa tcatcatgat gtgtgaaatc ccgtccaacg ccttgctggc
    21181 agagcagttc ctcgaatatt tcgacggctt ctcaattggc tcaaacgaca tgacacagct
    21241 ggcgctcggt ctggaccgtg actccggagt ggtgtctgaa ctgttcgatg agcgcaacga
    21301 tgcggtgaaa gcactgctgt cgatggcgat tcgtgccgcg aagaaacagg gcaaatatgt
    21361 cgggatttgc ggtcagggtc cgtccgacca tgaagacttt gccgcatggt tgatggaaga
    21421 ggggatcgat agcctgtctc tgaacccgga caccgtggtg caaacctggt taagcctggc
    21481 tgaactgaag aaataaaata aatccccggc ggcgttcagt cgccgggttt atgtgatccc
    21541 cgaagatgaa acttattcaa tctcttcaca gacatcctgc gttaatcgcc gcataatatc
    21601 ttttcttaac aaaaactttt gtattttacc tgaggcagtg cgcggtagtt tttcgattac
    21661 cacgatatgt tcaggatatt tatattttgc gacccgttta cggctaaaaa aagccaccac
    21721 ctcttccagc gataatgaat gatgcggcgc tttcagcacg acataagcgc atgatcgttc
    21781 acctaaacgt tcatcgggca ttgcaaccac acaggcatcg tgaattttag gatgctgcaa
    21841 taaaatatct tccacttcac ggctgctaat attttcgccg ccgcggacaa taatatcttt
    21901 tttgcgtccg gtaattttta tatagccagc ctcatccata cggcagagat cgccgctgta
    21961 ataccagcct tcttcatcca gcgcacgggc ggttaattca ggttcatcaa aatatcccat
    22021 aaacacattg gggccacgcg aggcttcttc accttcgcaa cctggcggta aggtcttgcg
    22081 tgcgtcatcg accactttaa tctctacacc tgcggcagcg taaccatcgg tgtgcataaa
    22141 gcgcgacaaa ggatcatcga gattcaccac cgcatgcggc gaactttctg tggaaccata
    22201 aacacttaat aatttaatgc cgagctgctg gcattcacgc gccacttttt tggggattgt
    22261 ggttccgccg caaagaaaga aacgcagcgc tgacaggtcc gcgggttgtt tctctagtac
    22321 attcaaaaga tcatagacaa acggcgttgc gcctagcata caggtgcaac gctgctgctc
    22381 aagcagcgcg agacacgcat caggagtgaa aatatctaac aacacgctgc gagcgccaat
    22441 taagaatggt gccgttacgc catgcagaaa gcccgttgcg tggccgagtg gcgcaggcat
    22501 cataaagaca tcctgccagg tcagattcag ccgcgcgcaa taagcccgct cactggcgag
    22561 aatattgtta tgcgttagca tcacgccctt tggcagaccc tcggttccgg aggtaaacag
    22621 caccgcagct aattcatcgc cgtgggtcgt tatcgccgtg gtcagtgggg tattgtcggc
    22681 gataatctga cttaatgaga gggaagaggt ggcgggagcc agtttgtcca cgccgacaat
    22741 ttgttgtagt tgtggaagct gattttgcag cggcaggatt aaatctaccg ggcgcgtttg
    22801 tttaaacaac gtcggtgcaa agaacatttt tgcctgacac ttattgagca cccacaccag
    22861 ttctgcttcc cgccaggaag gcaacagcgg cacggaaacc gcaccgattt tcaggcaggc
    22921 aagatagata acggtaaatt cacaccagcc aggcagttga aatgcgatgc gatcgcctga
    22981 ttcaataccc ttcgctaaca tccagtttgc cagacagctc gcggcgtgat cgagcgcgct
    23041 atagttgtac gatgcaccat gattatcgac cacggcaatt ttgtctggca tcgcacgagc
    23101 ggtctgctgc cagtaatcgg ccagcgaagc atcgccccat agcccttgct gacgatacgc
    23161 cgcacgacgt tgttcgttga acgttaatgt cactttcatg ttggactctc gaaacagaac
    23221 atcaggcccg agatgcgggc ctgtgggatg catgattagc cgtagcgaaa atcaataccg
    23281 aaggtgcctg cgggatattg ccactgttcg agaatagtgt taccgcacag cacccgacag
    23341 gtgccgcact ccagacaacc ggcagaatca aaatgaatgt tgccggcgtc atcctgctta
    23401 taaagtccgg cagggcaggc tttcattaat ttacggaatt cattgatatc gggatttgcc
    23461 gccagaatga tatgcgggtg gccctcatca acatggaatt tattgacgcc taatttgatg
    23521 tcgacgttaa ccgtagcgtt ctggctcata gcgcggttgc tcccttaatg ccatctttca
    23581 gcaagttgat cagcccaatt ttcttcgcat gtcccatgat cattttgcgt accggctggt
    23641 ttggtttgcc gtcaatggtg aacatctcgt tcatgatgtc ggcgaccatt cgtgggtatt
    23701 ggctaaacag gcgcgggttt tccatcagcg ccgggatctt gcggaaatgc tgcatatcgc
    23761 gcatgacgca gctttgttcc agctcacgtt tgtattgcgc cagactgctg gcggagaaat
    23821 cctcgcgttc tttggcggcg atcaccgttg tggcggcagc ctgagccgat gcaatggcta
    23881 aatccatgcc acgaactgta aaacccaaat tcaggcagaa gcctgcggcg tcaccaacga
    23941 tcatcacgcc atcgttaacc atctgcggca ccattgccag accgccttct ggcaccatat
    24001 gcccggaata ttcaagcagt ttgccgccgc taattagtgg gcgaatggcg gggtgttgtt
    24061 taaaatcttc cagcatttgc ggcacgcttt tttgcgcatg ggcgatatca cccaatccac
    24121 aaaccagccc caaggatatg gaatccttgt tggtatagag aaatcccccg cccatcaggc
    24181 cgtcagaagg ggaaccggca aacagccagg cggcaccttc ctcgcccgta atattaaagc
    24241 gatcgttgat ctgttctggt gtgaggccaa taacctcttt aacaccaaca gcgtaatgat
    24301 gcggatcgga agcgggaacc attcccagcg agcggccaag catcgagtta acgccatcag
    24361 ccagaatcac cacattcgct tcgagaatat catccccagc ctgcacgcca gtgaccttgt
    24421 ttccttcacg aaccagcgca tcgacgcgaa cgcctgggat aaactgcgcg cccgcctgct
    24481 cggcttgttc catcaaccac gggtccagac gattacgcaa tacggtataa gatgcgtgtt
    24541 gcggaacatc tggttgctcg cggtgaaaat cgagggtaac ggcgctttct tcggttaaga
    24601 aggagatttt ctcgcgtgtg accttgcgtt ctaccggcgc tgatactgca aagcctggaa
    24661 tgattgcttc aagtgtgtgg gcataaagac gcccgccggt catgttttta catccggcac
    24721 tgtcgccgcg ttctatcacc aggacatcca gcccggctcg cgccatgacc agtgcggcaa
    24781 cgctaccagc aacgcccgca ccgaccacaa tggcatcaaa tttgtcttcc gacatgcgtt
    24841 atgctccctt cccgctgcgt ttacagcggg agatgtggtt gaaaggggaa ttagcggctc
    24901 aactggctaa tcagggcagg gacgactttg tagatatcgc ccaccagacc gtagtcggca
    24961 taattgaaga ttggcgcatt tttatcttta ttgatggcga caatcacttt tgcgccgttg
    25021 ccgccaacca tatgctggat ctgcccggag atccccagcg tcaggtagag atcggatttc
    25081 agcaacacgc cggagacacc gatataacgt tcacgctcca tccagttctc gccttcggca
    25141 attggacgtg aacagccgac ttcagcattc agcaccgccg ccagttcgtg gaccattttc
    25201 aggtcatcct gcgccgccag accacgaccg acgccaacca cacgttttgc tttgctcagg
    25261 tccacgctgc ttgcggcttt cgcacggcgt tcgcgacaga gaatttcatg acgcggagca
    25321 acataaggta ccgtttctgt cgggcactga tgagaggtat cactggtgca cggttcctga
    25381 acaccgggtg caagggtaat gatcgccagc gggctgttga tcttttcctg agcgaacgct
    25441 aacccgccat acatccggtg ttcggcgcaa atgtgaccat cgacaatatc caccgccgtg
    25501 gcatcattca ccagcgccgc attcagttgc acacttaacc gtgctgccag agctttacca
    25561 cgtttcgtcg cggccaacag caacatagcg gggtgtttat ctttcagcag ggcagcaatg
    25621 ctttcggcgt aattttcagt gcgttgcagc gcgtcgtttt gctcaagaac ataaatacat
    25681 tttggaccat aaggcataac tgcctgcgcc tggtcggtat tttgtacaat ggcatacacc
    25741 tgttggcccc attgctgagc gccgccaaac agttcagcat aacgttcagg attatcgcta
    25801 aagacccaga cgctgtttaa ttgactcatc gtttcttctc cgtaggcttc agttcagggc
    25861 tttcttcaga tgttccgcca gctcggcaat ggcctccggc gaatcgttat cgataatgat
    25921 gtgcttacgt tctgtttgcg gcggtacgcg aatgccgacc agttcagcaa gtggcgcgct
    25981 ctggctccag tcaatatcac ttgcctgcca ctgatttacc ggttttttac ccgcgccgag
    26041 aatggctttc atcgaaggaa tgcgtggcac gttaatatcg gaggtgacgc agagcacggc
    26101 tggaacagag agttcaataa cttcaacatc atcttcaagc gtgcgttcaa tcaccagtgt
    26161 attgccctga cgctgaatag cactcactgc attaatcacc ggaagttgca gaatttctcc
    26221 gaccagcaag ccagcctgct gggcataaag gtcgccggaa ccttcaccaa agatcagtaa
    26281 atcgaagccg atcttttcaa ctgctgctgc cagcgctttt gcggtatcga gaggcagtgc
    26341 atgttcaagt tgcgcatcct gaaccaaata caggctgtgc ggcccgcggg atagcacgtc
    26401 tttgcgcact ttcgagttct gcaacaatga gccgccaacg gtcagcgcgg ctatctcatc
    26461 gtcatctgtt gcaagctggc ttgcagcttc aatggcattg agatcgaact ggctgatttt
    26521 ggcgtcggca ttgtcgaaat tcagggtgta ttctggagtg acaacaatgt cctgttcttc
    26581 aggcaccagc ttaaagcagg ttattatttt catgatttct cctgtgagtc gcatacggaa
    26641 ccatctccga tggaaggtaa aaatggcctg aaaatagtgt gcgatagcgg tgcggggttt
    26701 tcgacggagc aagctgtttt ccttgccaac aatcttgcaa aaaaaatgcg atcgctaaaa
    26761 caaaagtgca atttgcggca atatagttat tccatgaccg tacaatcttg cattcagtgt
    26821 aaataatctt gtttagctct cgctaaacgc ggttgtataa ccgcaataca cgccgtattt
    26881 tatccggcat atattgtttt tgcttgctca tggaataggg aggttgtcat gtatcaacgc
    26941 tgttttgata atgccagtga aacgctgttt gtcgccggta aaacgccacg gctttcacgt
    27001 tttgcaatta gcgatgatcc aaaatgggag tctggacatc acgttcatga caatgaaacc
    27061 gagctgattt acgtcaagaa aggggtagca aggtttacca tcgattcatc gttatatgtc
    27121 gcacatgccg atgatatagt ggtgatagaa cgcggcaggc tgcatgcggt ggcctctgac
    27181 gttaacgatc cggcaacgac gtgtacctgt gcgctgtacg gctttcagtt tcagggggct
    27241 gaggaaaatc agctactgca accgcattct tgtccggtaa ttgccgcggg gcagggaaaa
    27301 gaagtcataa aaaccttatt taatgagcta agtgtgattt tgccgcaaag taaaaatagc
    27361 caaacatctt cgttatggga tgcatttgcc tatacgttag ccattcttta ctacgaaaac
    27421 tttaaaaatg cttatcgttc ggagcagggg tatattaaaa aagatgttct gataaaagat
    27481 attctttttt atctgaataa taattatcgc gaaaaaatca ctttagaaca gttatcgaaa
    27541 aaatttcgtg ccagcgtcag ttatatttgc catgaattta ccaaagagta tcgtatttcc
    27601 cctattaact atgttattca acggcgtatg acggaagcga aatggtcact cactaatact
    27661 gaattatcac aggcagagat ctcctggcgt gtgggttatg aaaatgtcga tcactttgcc
    27721 aaactgtttt tgcgccatgt cggttgttcg cccagcgatt accgcaggca atttaaaaac
    27781 tgttttgcgg aacaagaaat cctatctgaa tttcctcaac cggtaagtct tgccgggtaa
    27841 atctggcggc gcagtttgtt aagaagctgc gccgcctgca gattatttgt tctgataatc
    27901 tttcaggatc tgccgacccg caacgtaaat cataatttcg tctgtaccgc cgccgatacg
    27961 ttcacaacgg acatcacgcc agaagcggga gacgcgcgcc tcatcggtat agcccagacc
    28021 gcccatgatt tgaatcgcat catcaatgac ttccattgcg gtacgtgcac agtacagttt
    28081 tgccagcgcc gcgctggtgc gcagtgactg atgctgatcg gcttgccatg ccactttcag
    28141 caccatgttg cgcatgttgt cgatcttaat cgccatcagc gccagttttt cctggatcat
    28201 ctggttatga ccaatgggct taccaaaagc gatacgttgg ttggcatagc gggcggcatc
    28261 ttcaaaggcg cattcggcaa agccggtgct gcgcgcggcg ttgatcaggc gctccatctc
    28321 aaagttgtac atcacattga ggaaacccat tccttcttcg cccaccatgt cgctctcttc
    28381 aacctccacg ttgtcgagat agacttcgca ggtgctgagc atatgccagc cgattttatg
    28441 cagcgggtta atcttaatgc cgggcttact ggagtcgacc caccacaggg tgaaggcttt
    28501 tttgggatct ttcggttgcg gatcgcgcgc caacaccagc atatacgggt actctttcgc
    28561 accggtaata aaggtttttt gtccgttgat gtagaccttg ccgtttttac gcgtgtaagt
    28621 ggtagtggca ctgttgttat ctgagccagc acctggttcc gtcaacgcca gggcataggc
    28681 gggatcaccc gtttccaggg tgctttctgc cgttttacgt agctgctctg cagaaccgaa
    28741 acggcgcata ctgtgaatac attgaccgtt ggtgatcaaa aatgccggag caccgcattt
    28801 tgacacttcc atcagcgcca gcatttgggt gacgtaatcc gcagggatac caccaaattc
    28861 ttccggcacg ccaagcatgg aaataccgtt atccgccagc gcccgcataa actcacgcgg
    28921 atatgtcccg ttttgatcgc aggtgcggaa atactcttcc ggaaagttag tcgtaatcag
    28981 ttcgcgaata ctggccagca gcagttcttg ttcttcagtt aaagaaaaat ccatcattat
    29041 actcctgtta atgagccgct tcaggcagga caaaacccat cgccgcatcg ataaataatc
    29101 tttcgtccat cagtttgagt tctggcgaaa tcactggggt gaagtccatt ttgtcgagaa
    29161 tatctttttg taaatcgacg ccaggggcga tttcgattaa atgcaggccg tcttctttca
    29221 gcgtgaatac agcacgttcg gtgatgtaac gaacatccag ttcccgttcg agcgcgattt
    29281 ttccgctaaa agtaatttcc ggtagttccc gaataaattt atttacccgt ccttcctgga
    29341 cgatatgcaa tttgccatcg gcaatttctg tttttaaact gcccgcagtt aacgtgccgc
    29401 aaaaaataat tttcttcgag gtggcactga tatcaataaa tccaccggta cccatgattt
    29461 tgccattgaa tttatgcacg ccgacgttac cgtgctggtc gacttcagca aaactcaaat
    29521 aacaaacatc cagaccacca ccgtgataaa aatcaaactg ggacgtcata tccagaatgg
    29581 cgcgggtatt cacgttcgcg ccaaaggcga taccctgtga agtaattccg ccaatcggac
    29641 ctgtttctac cgtcagaata aagtcatcag cacaaccttc ttcccgggcg actaggccaa
    29701 taccgtcagc aataccgacg ccgacattcc ccaccgcgcc tttacgcatt tcgaataatg
    29761 cacgcctcgc aactaattta cgttggttta ggggcaggct aagtttggtg ctgtcatcaa
    29821 gggtgaagtc accagaaata aagcggttaa ccggcgcacc gccatacagt tggctttgat
    29881 ccggatcgac caccacaata tccaccagat aacccggaat acggacagat ttaggatgca
    29941 gcgtggcttt cttaaccatt ttctgcacct gcatcatcac aataccgccg ttattgtgca
    30001 ccgcctgggc aataaccaat gcgtcgagat acatcacctc atcttcaaaa gtggcgtagc
    30061 cttcactgtc gcaggtggta gcgcgaatga aggcgatatc tggcgcaatc gctttgtaat
    30121 agagatattc tttgttatca aactcgacca gtttaatcag gtcttctttc gtgacttcat
    30181 tcagtttgcc gccttgctgg cgtggatcga caaatgtccc gatgccaata tcactaataa
    30241 taccaggctg gtgggctgcg gcggcgcgta aggtttgtgt aagtacacct tgtggatagt
    30301 tataagcaat aattttattt tgttctgcga gttcagaaat acgcggcgat tgtccccagt
    30361 gaccacataa tgcccatttc accagacctt cttgcgccag aggactaata ccacggtcgg
    30421 cgcgatcgcc aagccctgtc ggactaataa tcgataaatt acgtggcgtt tgagtctgtt
    30481 tatatttatc agcaagagct gtaattaacg tggtggcttc cagaataccg ccgccagcgc
    30541 ctaacacaca aagtgttgct tcgtcgggaa tataattcac cgcttcctgt gccgacagga
    30601 ccggcactcg tccattaata cgaggtggtt ttacaggttt cattgttaat tcctgattca
    30661 gatgtgattt tccctacgcc agatggagaa ttagcgcaca gagactcacg aaatattttt
    30721 attaaatacg gttaaatata ttttttaagt tcggcgggga gggtgttccc gccgaaatat
    30781 tattgcttat gcctggtgta aaatagttaa taccgtgcgt aaatcattta ccgagatttg
    30841 ccctggtgca gaggcttttt ttaccgcacc aaaagttgcc gccgagccaa atacttcacc
    30901 agccagacga gaaattacgc cagtttttgc catcgacatc gtgatgattg gacgatcggc
    30961 atactgctcc tgcatctcca gggtcgcggt aagcaacgtc agcacatcgc tggtactttg
    31021 cggcatcagc gcaatcttag gaatatcggc gtcgaaggac tgcattttgc gcagacgggc
    31081 aatgatttct tcggcttccg gcgttttatg gaagtcatgg ttggacatga caactttcac
    31141 atcatgcgcg tgggcgtagg cgacggtttc tttaacctga tcatcgccgg taaataactc
    31201 cagatcgatc atatcaacca ggccgctgtc gatggctgca cgattgagag caatataagc
    31261 ctcggtggaa atcgcctgct cgccgccttc tttggcactg cggaaggtaa acagcagcgg
    31321 tttttctggc atggtttcac ggagaatttt tgccgccgcc atgacagact ccacattgga
    31381 gaggtcggca aagtggtcca cacgccattc cagaatatca aagtccgctt cacgataggc
    31441 gagagcttcg gatttcacgc gggcgatatc tttcgccatc agcgagacga tgattttagg
    31501 tgcgcccgta ccaatgacga gatcttttac agttacggtt ttcatttttt accctttctg
    31561 cacgcggtca gcctgtcagg caccgaaccc catgacctgt ttaacatatt ccagagggaa
    31621 atctttgcca gtccataacg tgaactgttc agccccttgc cacaacaaca tgccgtatcc
    31681 atcaatcgtt ttgcaaccag cttgttgcgc ctgctgcaat aacttcgtca tatgcgggtt
    31741 atacacgcat tcagtgacca gtagtcccgg atgtaacaga ctgatatcat taactaatga
    31801 ttcattctca aggggtttca tacccacttt tgtgccattg gttaaaatgt cggcggaagc
    31861 cagggcttca gcaaaggctt gctgatcggc gagatcggta accgtgacga cacaatcggt
    31921 gttttcgtta acccgctgcg cgaaggcgag ggctttatcg aagaactcat cccgacggtt
    31981 aaagagttta atttctttta aaccttcaat tgccccctgc gcgccaatcg ccgttgaggc
    32041 acctccggcc cctaacagca ccattgtttt gcctttgata tcaaaaccgc tctctttaat
    32101 ggcgcgaata tggcccgtgc cgtcggtgtt atagccgcgc aggtagccat catcattaac
    32161 gatggtgttg atggcaccca ccagtttggc tgctggcgtt aattcatcaa catattcaca
    32221 cgccagttgt ttgttcggca ttgatatacc agttccgcgc attttgaggg cttttaatcc
    32281 ttcaattgct ccgggaaaac tatcgttatc cacttcaaag gccatatagg taaatggcaa
    32341 tcccgctttt tctaaggctt tattctgcat ttcaggcgat aaactgtggc ggataggata
    32401 ggccatcagc ccaatcaatt cgtattttgc ggtaacatcc ataattaact cctttaacct
    32461 ctatgcttaa ttgtgtttaa ccgggtgcta aatttgcgct cgccaaaccg cacatcattc
    32521 tcaggaataa taaaaacgcg gtaatagcgg ataaaaacaa taattgcggt aatcagggcc
    32581 agcagcgcga aagtaaaatc gagaacaatg atatattgca ggccgatgtt cgacagataa
    32641 ccggtaatca gcggaataac aaagttagcc agtccaccca tcatcatata aatactggtg
    32701 actttggctt tgctttttgg gaaaaactct gacatgaccg aaacaccgag ctgtaaaatg
    32761 ccgccagctg ctgagaaacc gataacaaag gcgccggcat tacacaccag tggagaaggg
    32821 tacaggtaga taatggctgc tgttattgtt gccagtgcag agttaaatac attagcccag
    32881 atgggccgga ccattttttt cagtagtgcg gcaaaaataa agacacagac caacgagccc
    32941 atactgtaat aagagatggt ttttaatgcc tcagcttctg acataccagc aaaagccatc
    33001 gcatatttgg gcatccacac cacaatcaca taaaaggtcg agaatgcggc tacaccgaac
    33061 agtaccgatg aaacaccttc cagccagact aacggtttgc tgttcatttg cggtaattca
    33121 ttagctacgc tggcgtccac caactggctg gggaatttgc ttttcaacag catcagcgtg
    33181 atcagtacaa acagaatacc cggaataatc agcccatagc cgtaccagat attattgagc
    33241 aacatatagc tcaccagcat tgggtagaac atttgcccaa atgacaccat cgctttaacc
    33301 agtatgaccg ccgaaccaga ggctttcgga aagcattcca tgagcgcggg gtagccaccc
    33361 gtatccagcg ctgagttagc gatacctacg cacactgcca gaccgtaggc gagagttaaa
    33421 ttcgggcaag cgggaatacc aaagaagaat agcagataca ttattactgc cattaatatc
    33481 accgcccgac gaccaaactt atcggagatc acaccgaaga ataaaatact gatcaatcgc
    33541 cccaaaccga taccggaaat taagtaggca atgcccgcgt tgtcagtgga aaacttttct
    33601 gccagagatg acatattttg ggcaagcgta ataacactaa tgccgtgcag gaaatagctg
    33661 aagtaaatac aaagaacagc caggataaat ggcgtgctga aagccttatt ttgagacatt
    33721 ttcacagctc cttgctgaaa gataatttcg ttaccgtacg tattgttctc cgacggttta
    33781 cataattact atactgaaga tgattttccc tgggtacatg gcataaagtt tatgtttcca
    33841 ggaatattgc atgtcatagt ttgtttttga tcgtgatttt ccttagttag cgcagaaaga
    33901 gttatttatc cgtaagatct tgcatgacct gaaaagaata ttgcattacc cacccggagc
    33961 gacattttcc tttagcgagt gatgccgcgt ttttttgcgg ctgcgtaagc cgataaacag
    34021 tgccagtaaa aagccgatgg cagcgatggc ggtatcgaac cacataatat cggcaatact
    34081 tctttgggac agatgagccg taatcaacgg aatagtaaag gtcgcaatac tgcccgcact
    34141 gtaatagatc cctgtagctt tacctttagc gtaggggaaa cgttcagcca ttaacgtcag
    34201 gccaatttgc acaacacctc cagcagaggt aaaaccaatg acaaaagcaa atattatcac
    34261 cacataaaat gtgggatgca ggcagacggt aaacagagca ataaatgaga taaaggtgta
    34321 cagcatcagt aatgttgttg ggcgaacggt atttcgaatg agtggagcgg taataaatac
    34381 acaaagcagc gaacccacgg tgtagatact gagtagtttg atcgacatag tgtatgacat
    34441 gcctgcaaca aattgtccgt actgtgacag ccactggcta accagataaa acgttgccat
    34501 tgagatatag ccatataagg tataactggc taaatcgata attgaacagc gatgttccgt
    34561 agagcgggtg gttttcttta tgacaggtaa gcgacgaccc ggatggggtg ggaacgtaca
    34621 acggtataaa aacagagcgt taataagcat aatgcctgca gcaatcataa aggaccaacc
    34681 gaaccacagt tcagcccaca ctaacaggct aatgattagc ggtaataaaa attgtccgct
    34741 ggaaacaaat gctttaatta aaatattggc tgtcccaggt gagcgtggaa aagcttccat
    34801 caaactggga taagtgcctg catcgagaaa actgtttgcc attcccgcca gaaagccaaa
    34861 aacataagcg ataatgatgt tattggtctg caggatgcca aaaaagaagg ccatatagca
    34921 acacatcccg agcatgataa aagggcggcg accaaagcga tcggataata atcctgcaaa
    34981 aagcaagaca cttaatcgac caatgcccag cgatgagata actatcgaga cacccgcggc
    35041 attagtctgc caaagtgtct ccagcgaggc catattcagg ctcatcaaaa tgacgcccat
    35101 accatgcacc aggtaattaa aatacaaccc aagtgcggta gggtaatagg gatttttcat
    35161 aggttctact tagcaaaggt gctttaaaaa gtgtagcgat gttgtgtcag tatccattca
    35221 ttattgttgt aacaacgaca attcctttct gtggaaaaat tagcaaagcc gctccagatc
    35281 gtgtgcatat aattcagcgg ctaccgattg atagatcttc cagtcaataa aattgccatc
    35341 agggtagact cgttgaaatg cggttaagtc ggggaaaaaa cgcatggtgt tgttgccgat
    35401 gcggttattt attttctcta tgatggcatg aagatgtttt tttctttgct gacgcatagc
    35461 caacagttgc tcgactacac gatcaggaat ggcacacttg ccattttccc attgacgcca
    35521 gctttcactg tcaccggtct gggcaatcca ggtcgcgcat tcatcaatag tcatggcaaa
    35581 aatatggcgg agtgcctgga gttcataagc gttcatcgtg taatggctgc atgaaataaa
    35641 caataaaata taaataacat tatcagcctg ctgacggctt gaagagagtc acagtatctt
    35701 gtgcaacatt attggaaaga gtgaggggcg aggtagcgaa gcggaaaaat gttaaaaaaa
    35761 agcccatcgt gggagatggg caaagactac acacagcaat tcgttgtttc actcagggga
    35821 tttccatggt tataaatcaa tatgttgatt tataaccgtg agctaatagt aggcatacgg
    35881 actttttgcg tcgatcagat tcgtctcatt agtttaaaaa ctgtaaagaa tttgcgacag
    35941 gtactaccag ctgaatggcg gtaagcagga aaatatgggg aagagcaagc aaatatcaat
    36001 tagcggtggt taacaatcag gcggcagagc cgcctgatga gaaattactt attcggtttc
    36061 tcgatttcgc caagttcttc gagaatttct tccggttggt ccgtcggcgg cgggacttct
    36121 tccacccacg cggcaaacag acgccaggaa acggctaaca gaaccggacc aataaacaga
    36181 ccgatcatcc cgaaagcaat caaaccacca ataacgccag agaggatcag gatcagcggt
    36241 aaatcggcac ccatgcgaat taacattggg cggatgacgt tatccagcgt gccaaccaca
    36301 ccgctccaca ctaacaatac cgttccccag gtggtatcgc cggtccagta gagccagata
    36361 atcgccggaa tcagtaccgg caacgggcca agctggacaa ggcaggagag gatcattaac
    36421 accgttagca acgttgcata aggtacgccg gatacggcga ggccgatacc gccaagcact
    36481 gcctgtacta acgccgtcac caccacaccc agcgccaccg cgcggatagc ctgtgccgcc
    36541 agcagcacgg cggcatctcc gcgaacgcct gccagacggg ttgcaaaatg gcgaatgcct
    36601 tgtgccacct gttcaccgcg ccagtacagc agggcactga agagaagcat cagcgcacaa
    36661 tgcaccataa agcgcccgat atgcgccgcc tgcccaacga accaggtggt ggtggtgcca
    36721 atataagggc ggactttcgc catgatcgcc gtccccccca tatccagcaa gttgtgccag
    36781 cctgcataca gcttcgcgcc aatcaccgga atggtattaa gccacgccag atcgggtaac
    36841 gtcatgtcgc cgctggaaat ggctttaatt agcgggccgc tgccgtcgac gatactgtta
    36901 accagcaaag caatagggat gataaacacc atcactaata acagcgtcat caccagaacg
    36961 gcgagagagc ggcggccaaa catgatcttt tgcaaacgta acaataccgg ccaggtggcg
    37021 ataaccaccg taccggccca tgcaaagccg agaataaagg gttgaacaat ccacagacat
    37081 gccacaatca tgatggctaa aaacagcacc gaaagcagaa tttgtgcgac atccctgggc
    37141 tgacgaacat ttaccataac tacttttcac cttttttgtg cgtcaaatcg tcgacgcgac
    37201 gtgaacacgc gaaactcacc tgcataatga tgagcaattt cagcggtttt taacaggccg
    37261 attctgccct taattctgat gggcgtgtta gaaaaaaatg tggtacaaca actcaggcaa
    37321 cacgcaaacg atttactcgt cgtatttcaa gttgcatgtg ctgcggctac gttcgttcac
    37381 cccagtcact tactattgta agctcctgga gttcactcgc ttgccgcctt cctgcaactc
    37441 gaaatccata gagtaaataa acatcacctt tcagtgcgct attgcagccg cctgaaagaa
    37501 tagtgtatcg acctacatca cagaccgcag gaaagggtca atataatgat tccacagatt
    37561 tcccaggcac ccggcgtcgt tcaactggtg cttaattttt tgcaagagct ggagcaacaa
    37621 ggttttaccg gcgatacggc gacaagttat gccgatcgtc tgacaatgtc gaccgacaac
    37681 agtatttacc aacttctccc cgatgcggtg gtatttccgc gttcaaccgc agatgtggcg
    37741 ctgatcgccc gtcttgccgc gcaggaacgc tattcatcgc tgatctttac cccccgcggc
    37801 ggcggcaccg gcactaacgg tcaggcgctc aaccagggga ttattgttga tatgtcccgc
    37861 catatgaacc gcatcatcga aattaaccct gaagagggct gggtgcgcgt tgaggccggg
    37921 gtgataaaag atcaactcaa tcagtacctg aaaccgttcg gctacttttt tgcgccggaa
    37981 ctttcgacca gcaaccgggc aacgctcggt gggatgatca ataccgatgc atccggtcag
    38041 ggatcgctgg tctatggcaa aacgtcggat cacgtacttg gcgtacgcgc ggtgttgttg
    38101 gggggcgata ttctcgatac gcaaccttta cccgtcgaac tggcggaaac gctgggtaaa
    38161 tccaatacca caatcgggcg aatttataac acggtttatc aacgttgccg tcagcaacgc
    38221 cagttaatca tcgacaactt ccccaaactt aaccgctttc ttaccggtta cgatctgcgt
    38281 catgtcttta acgatgagat gaccgagttc gacctgacgc gcattctgac gggttcagaa
    38341 gggacgctgg cctttattac cgaagcgcgg ctggatatta cgcccttgcc taaagtgcgc
    38401 cgtctggtga acgtcaaata tgactctttt gactccgcgc tgcgtaacgc gccgtttatg
    38461 gttgaggcgc gggcgctttc ggtagagacg gtggactcaa aagtgctgaa tctggcgcgg
    38521 gaagatattg tctggcattc cgtcagcgag ttgattaccg atgtgcctga caaagagatg
    38581 ctcgggctga acattgtgga gtttgctggt gatgatgagg cgctgattga cgagcgagtt
    38641 aatgctctct gtgtgcggct tgatgagctg attgccagcc aacaggcagg tgtgattggc
    38701 tggcaggtgt gccgcgagct ggcgggtgtt gaacgtatct atgcgatgcg taaaaaagcc
    38761 gttggtctgc ttggcaatgc caaaggtgcc gctaagccaa ttccgtttgc tgaggatacc
    38821 tgcgtaccgc cggaacatct ggccgattat attgctgaat ttcgcgcgct gctcgacagc
    38881 cacggcttaa gctacggtat gttcggtcac gtcgacgcag gtgtcttgca cgtccgtcca
    38941 gcgctggata tgtgcgatcc tcaacaagag attttgatga agcaaatctc tgatgacgta
    39001 gtggcgctga ctgcgaaata cggtggtttg ttgtggggcg agcacggcaa aggttttcgc
    39061 gctgaataca gcccggcgtt tttcggtgag gaactttttg cagaactgcg caaagtgaaa
    39121 gcggcatttg acccgcataa ccgactcaac ccagggaaga tttgcccgcc agaaggtctc
    39181 gatgcgccga tgatgaaagt ggacgcggtg aagcgcggta cattcgatcg gcagatcccc
    39241 attgcggtac gccagcagtg gcgcggtgcg atggagtgta acggcaacgg tttatgcttc
    39301 aactttgatg cccgtagtcc gatgtgtccg tcgatgaaga tcacccagaa ccggattcat
    39361 tcaccgaaag ggcgcgcaac gctggtgcgt gaatggctgc gtttgttggc ggatcgcggc
    39421 gttgatccac tcaaactgga acaagaactg cctgaatccg gcgtcagttt gcggacgtta
    39481 attgcccgca cgcgcaatag ctggcatgcg aataaaggcg aatatgactt ctcacacgaa
    39541 gtcaaagagg cgatgtcggg ctgtctggcc tgtaaagcgt gttcgaccca gtgccccatc
    39601 aaaattgatg tgccggagtt tcgctctcgt tttctgcagc tctatcacac ccgttattta
    39661 cgcccgctgc gcgaccacct cgtcgctacg gtcgagagct acgcgccgct gatggcacgc
    39721 gcgccgaaaa cctttaactt cttcattaac cagccgctgg tgcgcaaact ctcggaaaaa
    39781 catatcggca tggttgattt gccgctgctg tcggtcccct cgctacaaca acaaatggtg
    39841 gggcatcgct cggcaaacat gacgctggaa cagcttgaat ctctcaatgc agagcagaaa
    39901 gcgcgcacag tgttggtggt gcaggacccc tttaccagct attacgatgc gcaagtggtg
    39961 gcggattttg tccgtctggt cgaaaaatta ggtttccagc ctgtgttact gccattttcg
    40021 ccaaatggca aagcccagca tattaaaggt tttcttaatc gttttgcgaa gacggcgaaa
    40081 aagacggcgg atttcctcaa ccgtatggcg aagctgggta tgccaatggt gggcgtcgat
    40141 ccggcgctgg tactttgtta tcgcgatgaa tataaactgg cgctgggcga ggaacgtggc
    40201 gagtttaacg tcttactggc gaatgaatgg ctggcaagcg cacttgagtc acagccagtg
    40261 gctacagtca gcggtgaatc atggtatttc tttggtcact gtactgaagt taccgccttg
    40321 ccgggtgcgc cagcacaatg ggccgcgata tttgcccgtt ttggcgcgaa actggaaaat
    40381 gtcagcgtgg gttgctgcgg catggcaggg acttacggac atgaagcgaa aaaccataaa
    40441 aattcgctcg ggatctatga gttatcctgg catcaggcta tgcagcgact gccgcgtaac
    40501 cgctgtctgg cgaccggata ttcctgccgt agtcaggtca aacgggttga aggcacgggg
    40561 gtacgccatc ctgtgcaggc tttactggag attattaaat gatatggaaa cgaaaaatca
    40621 ccctggaagc actgaatgcc atgggtgaag gaaacatggt ggggttactg gatattcgct
    40681 ttgaacatat tggtgatgac acccttgaag cgacaatgcc agtagactcg cggacaaagc
    40741 agcctttcgg gttgctgcat ggaggcgcat ccgtggtgct ggccgaaagt atcggttccg
    40801 ttgccggtta tttatgtacc gaaggtgagc aaaaagtggt tggtctggaa atcaatgcta
    40861 atcacgtccg ctcggcacga gaagggcggg tgcgcggcat atgtaaaccg ttgcatctcg
    40921 gttcgcgtca ccaggtctgg cagattgaaa tcttcgatga gaaagggcgt ttgtgctgtt
    40981 cgtcacgatt gacgaccgcc attttgtgac gttgtcacca gaaaagtgtg acgcatattg
    41041 cttttggtca ttatctgcac agtaaacggt ggtatactgt gcaggtcttg tctaaaagcg
    41101 aggatgctat gtctactcaa cttgatccca cccagctggc cattgaattt ttacgccgtg
    41161 accagagcaa tctttcacct gcccaatatc tcaaacgtct gaaacaacta gaacttgaat
    41221 ttgctgacct gcttactctc tcttctgctg aattaaaaga agaaatctac tttgcctggc
    41281 gtctgggcgt gcattgatcc tgacttaatg ccgcaagttc gccaaatcga cctggattaa
    41341 cctccgtcca ggttcgatga ccttcttaat actgcttatc taccgcctga tattgttcaa
    41401 aaataatact aatcgcagtt tgtgttaaaa cggcgggtta gctttatgag attattctta
    41461 tcgccccttc aagagctaag ccactgagag tgccggagat aagcgccgga tggggtagaa
    41521 acccttaagc ctgtgttgca cagacttaag ggttttctta tttctgaacg ctgcactttt
    41581 ttacctatct cttaaatagt cattttcaat acaacttttt ctttttcatc acagccttaa
    41641 tgctccttag ataataacca ttatctaaca atgagatacc taattcttag cgtgcctgtt
    41701 aacccacaca tcagggtcta tgcttattaa aagcaggcaa aaagcatgtt ttacggtaaa
    41761 gcccctgcgt ttgctgggtt gtactgataa tcattatcac taacatgctg ttatacgctg
    41821 aaagcgatga agtgaggtaa atcgatggac atgcattcag gaacctttaa cccacaagat
    41881 ttcgcctggc aaggcttaac gctgacaccc gcagcggcga tacacatccg tgagctggtg
    41941 gcaaagcagc cgggtatggt cggcgtgcgc ttaggcgtga agcaaacggg ctgcgcgggc
    42001 tttggctatg tgctcgacag tgttagcgag ccggacaaag acgatctgct gtttgaacac
    42061 gacggcgcga agctgtttgt cccgctgcaa gcgatgccgt ttattgatgg cacggaagtc
    42121 gatttcgttc gtgaaggact taatcagata ttcaaatttc acaaccctaa agcccagaat
    42181 gaatgtggct gtggcgaaag ctttggggta taggcggtac tatgtctcgt aatactgaag
    42241 caactgacga tgtcaaaacc tggaccggcg gcccgctgaa ttataaagaa ggattcttca
    42301 cccagttagc caccgatgag ctggcaaagg ggataaacga agaggtggtg cgcgcaattt
    42361 cggcgaagcg taatgagccg gagtggatgc tgaagtttcg tctaaacgcc tatcgcgcat
    42421 ggctggagat ggaagaaccg cactggttga aagcgcacta cgacaagctg aattatcagg
    42481 attacagcta ctactcagca ccatcgtgcg gtaattgtga cgacacttgc gcgtctgaac
    42541 ctggcgcggt gcagcaaact ggcgcgaacg cctttttaag taaagaggtg gaggcggcgt
    42601 ttgagcagtt gggcgttccc gtgcgggaag gcaaagaggt ggcggtggat gccattttcg
    42661 actcagtttc ggttgccact acttatcgcg aaaaactggc ggagcaggga attattttct
    42721 gttcctttgg tgaggcgatc cacgatcacc cggaactggt gcgtaaatat ctcggcaccg
    42781 tggtgccggg gaatgacaac ttctttgccg cgcttaatgc ggcggtagcc tctgacggta
    42841 cgtttattta tgtgcctaaa ggcgtgcgct gcccgatgga actttccacc tattttcgca
    42901 ttaacgcgga aaaaaccggg cagtttgagc gcaccattct ggtggccgac gaagacagct
    42961 acgtcagcta cattgaaggc tgttccgctc cggtgcgtga cagctatcag ttacacgcgg
    43021 cagtggtgga agtcatcatc cataaaaacg ccgaggtgaa atattccacg gtacaaaact
    43081 ggttccctgg cgataacaac accggcggta ttctcaactt cgtcaccaag cgtgctttgt
    43141 gcgaaggcga aaacagcaaa atgtcatgga cgcaatcaga aaccgggtcc gcgattacgt
    43201 ggaaatatcc cagctgcatt ttgcgcggcg ataactccat tggtgagttt tactcagtgg
    43261 cgctgaccag cggtcatcag caagcggata ccggcaccaa gatgatccac atcggtaaaa
    43321 acaccaaatc gaccattatc tcgaaaggga tctctgccgg acatagtcag aacagttatc
    43381 gcggcttagt gaaaatcatg ccgacggcaa ccaatgcgcg caatttcact cagtgcgact
    43441 caatgctgat tggcgctaat tgtggggcgc ataccttccc gtatgtcgaa tgtcgcaata
    43501 acagcgcaca actggagcac gaggcaacga catcacgtat tggtgaagat caactgtttt
    43561 actgcctgca acgcgggatc agcgaagaag acgccatctc gatgattgtt aacggtttct
    43621 gcaaagacgt gttctcagaa ctgccgctgg aatttgccgt tgaagcacaa aaactcctcg
    43681 ccatcagtct tgaacacagc gtcggataag gaataaacat gttaagtatt aaagaattac
    43741 acgtcagcgt ggaagataaa gctatcctgc gtggattaag cctcgccgtt cgtcccggcg
    43801 aagttcacgc cattatgggg ccaaacggtt cgggcaaaag taccttatcg gcaacgcttg
    43861 ccgggcgcga agattatgaa gtgacgggcg gcacggttga gttcaaaggc aaagatttgc
    43921 ttgcactgtc gccggaagat cgtgcaggcg aaggcatctt tatggccttc cagtatccgg
    43981 tggagattcc aggcgtcagt aaccagtttt tcctgcaaac ggcacttaat gcagtgcgca
    44041 gctatcgcgg ccaggaaacg ctcgaccgct ttgattttca ggatttgatg gaagagaaaa
    44101 tcgctctcct gaagatgccg gaagatttat taacccgttc ggtaaacgtt ggtttttccg
    44161 gcggcgagaa aaagcgcaac gatattttgc aaatggcggt gctggaaccg gagttatgca
    44221 ttcttgatga gtcggactcc gggctggata ttgacgcata aaaagtggtc gccgatggcg
    44281 tgaactcgct gcgtgatggc aagcgctcat tcatcattgt tacgcactac caacgcattc
    44341 tcgactacat caagcctgat tacgtccatg tgctatatca gggacgaatt gtgaaatccg
    44401 gcgatttcac gttggtcaaa caactggagg agcagggtta tggctggctt accgaacagc
    44461 agtaacgcgc tgcaacagtg gcatcacttg tttgaagctg aagggacaaa acgctcaccg
    44521 caagcacagc agcatttaca acaattgctg cgtaccggac tgccgacacg taaacatgaa
    44581 aactggaaat atacgccgct ggaagggctg accaatagcc agtttgtcag cattgcggga
    44641 gagatatccc cacagcagcg agatgcctta gcgttaacgt tagacgctgt gcggctggta
    44701 tttgtcgatg gacgttacgt gtcagcactg agcgatgcga ctgaaggcag cgggtatgaa
    44761 gtgagcatta acgacgaccg tcagggggta cccgacgcta ttcaggcgga agtgtttctg
    44821 catttgacgg aaagcttggc acaaagcgtg acgcatatcg ccgtgaagcg cggtcaacgg
    44881 ccggcaaagc cattgctgtt aatgcatatt acccagggcg tggcaggtga agaggtcaac
    44941 actgcccatt accgacatca tctggatctg gcggaaggtg ccgaagcaac ggtgatcgaa
    45001 cattttgtca gccttaatga tgctcgtcac ttcaccggcg cacggttcac tatcaatgtc
    45061 gcagcgaacg cccacttgca gcatatcaag ctggcgtttg aaaacccggt cagtcaccac
    45121 tttgcccata acgatttgtt gctggctgac gatgccaccg catttagcca tagtttcctg
    45181 ctgggtggcg cagtgttacg acacaacacc agtacgcaac tcaatggcga aaacagcacg
    45241 ctgcggatca atagcctggc gatgccggtg aaaaacgagg tgtgtgatac ccgtacctgg
    45301 ctggaacaca ataaaggttt ttgtaacagc cgacagttgc acaaaactat cgtcagcgac
    45361 aaaggccgcg cggtatttaa cggtttgatc aacgtcgcgc agcacgccat caaaacggat
    45421 ggtcagatga ccaacaataa tctgctgatg ggcaaactgg cagaagtgga tacgaaaccg
    45481 cagctggaaa tctatgcaga tgatgtgaaa tgcagccacg gcgcgacggt ggggcgtatt
    45541 gatgatgaac agatgttcta tctgcgctcg cgcgggatca atcagcagga tgcccagcag
    45601 atgatcattt acgcctttgc tgccgaactg acggaagcac tgcgtgatga ggggcttaaa
    45661 cagcaggtgc tggcccgaat cggtcaacga ctgccaggag gtgcaagatg actttttccg
    45721 tcgacaaagt gcgggccgac tttccggtgc tttcgcgtga ggtaaacggt ttgccgctgg
    45781 cttatctcga cagcgccgcc agtgcgcaga aaccaagcca ggtgattgac tccgaagccg
    45841 agttttatcg tcacggctac gcggcggtgc atcgcggtat tcatacctta agcgcccagg
    45901 cgaccgagaa aatggagaac gtgcgcaagc gggcatcgct gtttattaat gcccgttcgg
    45961 cggaagagct ggtgttcgtc cgcggcacga cggaagggat caatctggtc gccaatagtt
    46021 ggggcaacag caacgtgcgg gcgggcgata acatcatcat cagtcagatg gagcaccacg
    46081 ctaacattgt tccctggcag atgctttgcg cacgcgttgg cgcagagctg cgtgtgatcc
    46141 cgctcaatcc cgacggtacg ctgcaactgg agacgctacc taatctgttc gatgagaaaa
    46201 ctcgcctgct ggcaattact catgtctcca acgtgcttgg cacagaaaat cccctggcgg
    46261 aaatgatcac gcttgcgcat cagcatggcg caaaagtgct ggtggatggc gctcaggcgg
    46321 tgatgcatca tccggtggac gttcaggcgc tggattgcga tttttacgta ttctccgggc
    46381 ataaactgta tggccccacc ggaattggca ttctttatgt gaaagaagcc ttgttgcagg
    46441 agatgccgcc gtgggaaggg ggcggttcta tgatcgccac cgtcagcctg agtgaaggca
    46501 ctacctggac caaagcacca tggcggtttg aagccggtac acccaatacc gggggcatca
    46561 ttggtcttgg cgcggcgctg gagtatgttt cggcgctggg gcttaataac atagccgagt
    46621 atgaacagaa tctgatgcac tacgcgctat cacagctgga atctgtaccg gatctcactc
    46681 tctatggccc gcggaacagg cttggcgtta ttgcttttaa tcttggtaaa caccacgcct
    46741 atgatgttgg cagttttctc gataactacg gcattgctgt gcgtaccgga catcactgcg
    46801 ctatgccatt aatggcctat tacaacgtcc ctgctatgtg tcgggcgtcg ctggccatgt
    46861 ataacaccca tgaagaagtg gatcgtctgg tgaccggact gcaacgtatt caccgtctgc
    46921 tgggataaca gggaggcact atggctttat tgccggataa agaaaaattg ctgcgtaatt
    46981 ttttacgctg cgccaactgg gaagagaaat atctctacat tattgagctg ggccagcgtc
    47041 tgccagaatt acgcgccgaa gaccgaagcc cacaaaatag cattcagggc tgtcagagtc
    47101 aggtgtggat tgtcatgcgc cagaatgcgc agggaattat tgaattgcag ggcgacagcg
    47161 atgcggcgat tgtgaaaggg cttattgcgg tcgtctttat tctctacgat cagatgacgc
    47221 cgcaggatat tgtcaatttc gatgtgcgtc cgtggtttga aaaaatggcg ctcacccaac
    47281 atctcacccc atctcgttca caaggtctgg aagcgatgat tcgcgcaatt cgcgccaaag
    47341 ccgctgcact tagctaaact aaagggacag ctttcatccg gttggctcac attgccggat
    47401 ggtcaatgtt tacactgacc tgcacaggag tgttttggtt tcaggtgaac ataagcgcca
    47461 tccgacgttt cagcgtgagt ctccggcaaa tacaggaggt ttacaatgaa acgcgcgtct
    47521 ttgcttacac tcacgcttat cggcgctttt agcgccatcc aggctgcctg ggcggttgat
    47581 tatccgctac caccaaccgg aagccgactg gttgggcaaa atcaaacgta tacggtgcaa
    47641 gaaggggata aaaaccttca ggctattgcc cgacgttttg atactgcggc aatgttgatc
    47701 cttgaagcca ataacactat cgccccggtg ccaaaacctg gtacgacgat aactattcct
    47761 tcacaactgt tattacctga tgcaccgcgt caggggatta tcgttaacct tgcagagctg
    47821 cgcctttatt attatccgcc gggagaaaat attgtgcagg tttatccaat aggtattgga
    47881 ttgcaggggc tggaaacgcc ggtgatggaa acgcgtgttg ggcagaaaat ccctaaccca
    47941 acctggacgc ctacggcagg cattcgtcaa cgttcgctgg agcgtggcat taaattaccg
    48001 ccagtcgttc ctgccggacc aaataacccg ctaggacgtt acgcactgcg cctcgcgcat
    48061 ggtaatggcg aatacctcat tcatggtacc agtgcgccgg acagcgtcgg tttgcgcgtc
    48121 agttcagggt gtattcgcat gaatgcaccg gatattaaag ccttgttctc cagcgtgcgg
    48181 acgggaacgc cggtgaaagt gatcaacgaa ccggtgaaat attccgtgga acctaacggg
    48241 atgcgttatg ttgaagtaca tcgaccacta tcagcagaag aacagcaaaa cgttcagaca
    48301 atgccataca cactgccagc aggctttacg caatttaaag acaataaggc tgtagatcag
    48361 aagttagtcg ataaagcgtt gtatcgtcgg gcagggtatc cggtttcggt gagcagtgga
    48421 gcaactcccg cagccagcaa tgcgccttca gtagagtcag cgcagaatgg tgaaccagag
    48481 caagggaata tgttacgcgc gacgcagtag cggtaaacgg gagacaaaaa aaatggcgca
    48541 caatgtgcgc catttttcac tgcacaggta ctattacttg cggtatttag tagccatgtt
    48601 gtccagacgc tggttagcac gagctgcgtc atctttagca gcctgaacgt cggaacgcat
    48661 tgcgttcacg tcgttgctca gctggtcaac tttagcgttc agagtctgaa cgtcagaaga
    48721 cagctgatcg attttagcgt tgctggagca acctgccagc agagtagaac ccaggattac
    48781 cgcgcccagt accagtttag tagctttcat tattaatacc ctctagattg agttaatctc
    48841 catgtagcgt tacaagtatt acacaaagtt ttttatgttg agaatatttt tttgatggga
    48901 aggcacttat ttttgatcgt tcgctcaaag aagcatcgaa cgctgggttt tagataaaaa
    48961 aattgagaga aaacaggatg cttccatcgg attcatctta gataaagtga gattatatag
    49021 tgaaatccga tttgattttt tattgcttct ggttatcgat taaataaaaa aagcgcccat
    49081 cagggcgctt cgatatacaa attaattcac aaaagcaata ttacaggacg tgaacagatg
    49141 cggtgttagt agtgccgctc ggtaccagtg caccagaaac cataactaca acgtcacctt
    49201 tgtgtgccag accgctctgc agagccagtt ctttacccag acggtagaaa tcatcagtag
    49261 aagtgatctc tttaaccagc tgcggcacaa cgcctttgct cagtaccaac tgatgagccg
    49321 ttttttcgtt ggtggtcagt gccaggatgg tggcatccgg gaagtattta cgtactgcgc
    49381 gagcagattt accgccctga gtagcaacca cgatcagcgg agcatccagt ttttcagcag
    49441 tttcaacggc accacggcat accgcttcgg taatgcgcag tttacggttg tcattgttga
    49501 actcgagacg gctgttcatc acgcggtcgg tacgttcgca aatggtcgcc atgatagaaa
    49561 ccgcttccag cgggtattta ccttttgcgg attcaccaga cagcatcact gcatcagtac
    49621 cgtcgaggat ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga
    49681 tcatggaatc cagcatctgg gtcgcagtga taacgacttt acgtgcacgg atacattttt
    49741 cgatcatcat cttctgggcg aagataactt cttctaccgg gatttctaca cccaggtcgc
    49801 cacgcgcaac catgatgccg tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt
    49861 cctggttttc gattttggag atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct
    49921 cacggatttc gataacgtca gaacgcttac gaataaagga agcagcaaca aagtctacgc
    49981 cttgttcgca accaaagatc aggtcctgtt tgtctttttc agccagtgct ggcagagcaa
    50041 tggaaacgcc aggcaggttc acacctttgt tttcgcccag gtcaccgttg ttcagcactt
    50101 tacagataac tttgttacct tcaatggcgg taacttccat accgatcaga ccatcgtcaa
    50161 ccagtacggt gttgccaact gacaggtcag tagtgaaacc ttcatacgtt accgcaacca
    50221 tttcgctgtt gccgataaca gatttatcag tggtgaaagt aaaggtctga ccagctttca
    50281 gagaaacgtc gttaccgcct tccagtttca tggtgcggat ttccggacct ttggtatcaa
    50341 gcaggatagc ggcggtttta ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct
    50401 gaccgtgttc tgcatagtca ccatgagaga agttcagacg cataacgttc atgccagcgt
    50461 ccagcatttt agctaacatc tcttcagatt cggttttcgg tccgatggtg caaacaattt
    50521 tggtcttttt catgacagtc ttagtcttta agttgagaag gatgggagaa acttgctttc
    50581 tgggcgcact gccggaaagc gaatccggta ttacgaaagt ggtggtgcct cgctctaagg
    50641 ataggtgaca tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag
    50701 tgcctgtttt tgagtcgttg agttctacag tccaaagtgc tgaaaccatt caagagtcaa
    50761 ttggcgcgca ttatacgctg aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa
    50821 atatacaact tgttagacaa taaattgtta tcaaagattt aacgttccag ggaaaaggtt
    50881 acgcaaaaac ttcactgttt gcgcaacgtc tggtgccgaa gttgtcagtg tgaggataag
    50941 tgaaagttca tccggtgtat aaatacatca tcgctcaatt gctacagtta aaataattta
    51001 tcgcgttatt tgtctgattt tgtttgattt ttgtttaatt ggttagattg atatatgctg
    51061 atagttggca tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt
    51121 accgagaaat gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg
    51181 aaccaaagca tattgttatc gcaggcgtat tacgtacagc attagccaat aaacgcattc
    51241 agcgcagtga gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac
    51301 cattcaggat gaggcggtaa gcttcatcct gatatttttg cttcagttgc ggtccagtta
    51361 atcattctaa ataacaactg ctgaaattat acgaatcgcg gtatgtattt taatttataa
    51421 cgataatatt atttttattt tctgcaatga acgtcacaaa taattgtcgc tta
//
LOCUS       DAZGAN020000023        50158 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGAN020000023 DAZGAN020000000
VERSION     DAZGAN020000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 50158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.023"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(113..400)
                     /gene="yebO"
                     /locus_tag="ACHKYO_RS07690"
                     /old_locus_tag="pgaptmp_001538"
     CDS             complement(113..400)
                     /gene="yebO"
                     /locus_tag="ACHKYO_RS07690"
                     /old_locus_tag="pgaptmp_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HGU4428402.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(475..618)
                     /gene="mgrB"
                     /locus_tag="ACHKYO_RS07695"
                     /old_locus_tag="pgaptmp_001539"
     CDS             complement(475..618)
                     /gene="mgrB"
                     /locus_tag="ACHKYO_RS07695"
                     /old_locus_tag="pgaptmp_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HGU4428403.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            777..1016
                     /gene="yobH"
                     /locus_tag="ACHKYO_RS07700"
                     /old_locus_tag="pgaptmp_001540"
     CDS             777..1016
                     /gene="yobH"
                     /locus_tag="ACHKYO_RS07700"
                     /old_locus_tag="pgaptmp_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HGU4428404.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1159..1950)
                     /gene="kdgR"
                     /locus_tag="ACHKYO_RS07705"
                     /old_locus_tag="pgaptmp_001541"
     CDS             complement(1159..1950)
                     /gene="kdgR"
                     /locus_tag="ACHKYO_RS07705"
                     /old_locus_tag="pgaptmp_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HGU4428405.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2127..3500
                     /gene="yebQ"
                     /locus_tag="ACHKYO_RS07710"
                     /old_locus_tag="pgaptmp_001542"
     CDS             2127..3500
                     /gene="yebQ"
                     /locus_tag="ACHKYO_RS07710"
                     /old_locus_tag="pgaptmp_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4428406.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3546..4427)
                     /gene="htpX"
                     /locus_tag="ACHKYO_RS07715"
                     /old_locus_tag="pgaptmp_001543"
     CDS             complement(3546..4427)
                     /gene="htpX"
                     /locus_tag="ACHKYO_RS07715"
                     /old_locus_tag="pgaptmp_001543"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HGU4428407.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4619..6667)
                     /gene="prc"
                     /locus_tag="ACHKYO_RS07720"
                     /old_locus_tag="pgaptmp_001544"
     CDS             complement(4619..6667)
                     /gene="prc"
                     /locus_tag="ACHKYO_RS07720"
                     /old_locus_tag="pgaptmp_001544"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HGU4428408.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6687..7385)
                     /gene="proQ"
                     /locus_tag="ACHKYO_RS07725"
                     /old_locus_tag="pgaptmp_001545"
     CDS             complement(6687..7385)
                     /gene="proQ"
                     /locus_tag="ACHKYO_RS07725"
                     /old_locus_tag="pgaptmp_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HGU4428409.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7482..7979)
                     /gene="msrC"
                     /locus_tag="ACHKYO_RS07730"
                     /old_locus_tag="pgaptmp_001546"
     CDS             complement(7482..7979)
                     /gene="msrC"
                     /locus_tag="ACHKYO_RS07730"
                     /old_locus_tag="pgaptmp_001546"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HGU4428410.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8109..9392
                     /gene="yebS"
                     /locus_tag="ACHKYO_RS07735"
                     /old_locus_tag="pgaptmp_001547"
     CDS             8109..9392
                     /gene="yebS"
                     /locus_tag="ACHKYO_RS07735"
                     /old_locus_tag="pgaptmp_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HGU4428411.1"
                     /translation="MALNTPQITPTKKITVRSIGEEQPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9361..11994
                     /gene="yebT"
                     /locus_tag="ACHKYO_RS07740"
                     /old_locus_tag="pgaptmp_001548"
     CDS             9361..11994
                     /gene="yebT"
                     /locus_tag="ACHKYO_RS07740"
                     /old_locus_tag="pgaptmp_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HGU4428412.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12074..13513
                     /gene="rsmF"
                     /locus_tag="ACHKYO_RS07745"
                     /old_locus_tag="pgaptmp_001549"
     CDS             12074..13513
                     /gene="rsmF"
                     /locus_tag="ACHKYO_RS07745"
                     /old_locus_tag="pgaptmp_001549"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HGU4428413.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13631..13867
                     /gene="yebV"
                     /locus_tag="ACHKYO_RS07750"
                     /old_locus_tag="pgaptmp_001550"
     CDS             13631..13867
                     /gene="yebV"
                     /locus_tag="ACHKYO_RS07750"
                     /old_locus_tag="pgaptmp_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HGU4428414.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13972..14163
                     /gene="yebW"
                     /locus_tag="ACHKYO_RS07755"
                     /old_locus_tag="pgaptmp_001551"
     CDS             13972..14163
                     /gene="yebW"
                     /locus_tag="ACHKYO_RS07755"
                     /old_locus_tag="pgaptmp_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HGU4428415.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14164..14820)
                     /gene="pphA"
                     /locus_tag="ACHKYO_RS07760"
                     /old_locus_tag="pgaptmp_001552"
     CDS             complement(14164..14820)
                     /gene="pphA"
                     /locus_tag="ACHKYO_RS07760"
                     /old_locus_tag="pgaptmp_001552"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU4428416.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15216..15557)
                     /gene="yebY"
                     /locus_tag="ACHKYO_RS07765"
                     /old_locus_tag="pgaptmp_001553"
     CDS             complement(15216..15557)
                     /gene="yebY"
                     /locus_tag="ACHKYO_RS07765"
                     /old_locus_tag="pgaptmp_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HGU4428417.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15570..16442)
                     /gene="copD"
                     /locus_tag="ACHKYO_RS07770"
                     /old_locus_tag="pgaptmp_001554"
     CDS             complement(15570..16442)
                     /gene="copD"
                     /locus_tag="ACHKYO_RS07770"
                     /old_locus_tag="pgaptmp_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HGU4428418.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNVSRLLFMLTTAQFILLAGVGHATMNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16446..16820)
                     /gene="yobA"
                     /locus_tag="ACHKYO_RS07775"
                     /old_locus_tag="pgaptmp_001555"
     CDS             complement(16446..16820)
                     /gene="yobA"
                     /locus_tag="ACHKYO_RS07775"
                     /old_locus_tag="pgaptmp_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HGU4428419.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16959..17189
                     /gene="holE"
                     /locus_tag="ACHKYO_RS07780"
                     /old_locus_tag="pgaptmp_001556"
     CDS             16959..17189
                     /gene="holE"
                     /locus_tag="ACHKYO_RS07780"
                     /old_locus_tag="pgaptmp_001556"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HGU4428420.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17291..17947
                     /gene="yobB"
                     /locus_tag="ACHKYO_RS07785"
                     /old_locus_tag="pgaptmp_001557"
     CDS             17291..17947
                     /gene="yobB"
                     /locus_tag="ACHKYO_RS07785"
                     /old_locus_tag="pgaptmp_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HGU4428421.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17971..18633
                     /gene="exoX"
                     /locus_tag="ACHKYO_RS07790"
                     /old_locus_tag="pgaptmp_001558"
     CDS             17971..18633
                     /gene="exoX"
                     /locus_tag="ACHKYO_RS07790"
                     /old_locus_tag="pgaptmp_001558"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HGU4428422.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18630..20690)
                     /gene="ptrB"
                     /locus_tag="ACHKYO_RS07795"
                     /old_locus_tag="pgaptmp_001559"
     CDS             complement(18630..20690)
                     /gene="ptrB"
                     /locus_tag="ACHKYO_RS07795"
                     /old_locus_tag="pgaptmp_001559"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HGU4428423.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASHDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFYAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEGGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20899..21558)
                     /gene="yebE"
                     /locus_tag="ACHKYO_RS07800"
                     /old_locus_tag="pgaptmp_001560"
     CDS             complement(20899..21558)
                     /gene="yebE"
                     /locus_tag="ACHKYO_RS07800"
                     /old_locus_tag="pgaptmp_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU4428424.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21885..22241)
                     /gene="yebF"
                     /locus_tag="ACHKYO_RS07805"
                     /old_locus_tag="pgaptmp_001561"
     CDS             complement(21885..22241)
                     /gene="yebF"
                     /locus_tag="ACHKYO_RS07805"
                     /old_locus_tag="pgaptmp_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HGU4428425.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22308..22598)
                     /gene="yebG"
                     /locus_tag="ACHKYO_RS07810"
                     /old_locus_tag="pgaptmp_001562"
     CDS             complement(22308..22598)
                     /gene="yebG"
                     /locus_tag="ACHKYO_RS07810"
                     /old_locus_tag="pgaptmp_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HGU4428426.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22732..23910
                     /gene="purT"
                     /locus_tag="ACHKYO_RS07815"
                     /old_locus_tag="pgaptmp_001563"
     CDS             22732..23910
                     /gene="purT"
                     /locus_tag="ACHKYO_RS07815"
                     /old_locus_tag="pgaptmp_001563"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HGU4428427.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23966..24607)
                     /gene="kdgA"
                     /locus_tag="ACHKYO_RS07820"
                     /old_locus_tag="pgaptmp_001564"
                     /gene_synonym="eda"
     CDS             complement(23966..24607)
                     /gene="kdgA"
                     /locus_tag="ACHKYO_RS07820"
                     /old_locus_tag="pgaptmp_001564"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGU4428428.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24644..26455)
                     /gene="edd"
                     /locus_tag="ACHKYO_RS07825"
                     /old_locus_tag="pgaptmp_001565"
     CDS             complement(24644..26455)
                     /gene="edd"
                     /locus_tag="ACHKYO_RS07825"
                     /old_locus_tag="pgaptmp_001565"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HGU4428429.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PRIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26690..28165)
                     /gene="zwf"
                     /locus_tag="ACHKYO_RS07830"
                     /old_locus_tag="pgaptmp_001566"
     CDS             complement(26690..28165)
                     /gene="zwf"
                     /locus_tag="ACHKYO_RS07830"
                     /old_locus_tag="pgaptmp_001566"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGU4428430.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28503..29372
                     /gene="hexR"
                     /locus_tag="ACHKYO_RS07835"
                     /old_locus_tag="pgaptmp_001567"
                     /gene_synonym="yebK"
     CDS             28503..29372
                     /gene="hexR"
                     /locus_tag="ACHKYO_RS07835"
                     /old_locus_tag="pgaptmp_001567"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HGU4428431.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29500..30942
                     /gene="pyk"
                     /locus_tag="ACHKYO_RS07840"
                     /old_locus_tag="pgaptmp_001568"
     CDS             29500..30942
                     /gene="pyk"
                     /locus_tag="ACHKYO_RS07840"
                     /old_locus_tag="pgaptmp_001568"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGU4428432.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVMEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31073..32044)
                     /gene="lpxM"
                     /locus_tag="ACHKYO_RS07845"
                     /old_locus_tag="pgaptmp_001569"
                     /gene_synonym="msbB"
     CDS             complement(31073..32044)
                     /gene="lpxM"
                     /locus_tag="ACHKYO_RS07845"
                     /old_locus_tag="pgaptmp_001569"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU4428433.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32164..33486)
                     /gene="mepM"
                     /locus_tag="ACHKYO_RS07850"
                     /old_locus_tag="pgaptmp_001570"
     CDS             complement(32164..33486)
                     /gene="mepM"
                     /locus_tag="ACHKYO_RS07850"
                     /old_locus_tag="pgaptmp_001570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HGU4428434.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33502..34434)
                     /gene="znuA"
                     /locus_tag="ACHKYO_RS07855"
                     /old_locus_tag="pgaptmp_001571"
     CDS             complement(33502..34434)
                     /gene="znuA"
                     /locus_tag="ACHKYO_RS07855"
                     /old_locus_tag="pgaptmp_001571"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HGU4428435.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34513..35268
                     /gene="znuC"
                     /locus_tag="ACHKYO_RS07860"
                     /old_locus_tag="pgaptmp_001572"
     CDS             34513..35268
                     /gene="znuC"
                     /locus_tag="ACHKYO_RS07860"
                     /old_locus_tag="pgaptmp_001572"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HGU4428436.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35265..36050
                     /gene="znuB"
                     /locus_tag="ACHKYO_RS07865"
                     /old_locus_tag="pgaptmp_001573"
     CDS             35265..36050
                     /gene="znuB"
                     /locus_tag="ACHKYO_RS07865"
                     /old_locus_tag="pgaptmp_001573"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HGU4428437.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36197..37207)
                     /gene="ruvB"
                     /locus_tag="ACHKYO_RS07870"
                     /old_locus_tag="pgaptmp_001574"
     CDS             complement(36197..37207)
                     /gene="ruvB"
                     /locus_tag="ACHKYO_RS07870"
                     /old_locus_tag="pgaptmp_001574"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGU4428438.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37216..37827)
                     /gene="ruvA"
                     /locus_tag="ACHKYO_RS07875"
                     /old_locus_tag="pgaptmp_001575"
     CDS             complement(37216..37827)
                     /gene="ruvA"
                     /locus_tag="ACHKYO_RS07875"
                     /old_locus_tag="pgaptmp_001575"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGU4428439.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(37966..38031)
                     /gene="yobI"
                     /locus_tag="ACHKYO_RS07880"
                     /old_locus_tag="pgaptmp_001576"
     CDS             complement(37966..38031)
                     /gene="yobI"
                     /locus_tag="ACHKYO_RS07880"
                     /old_locus_tag="pgaptmp_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HGU4428440.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            38102..38704
                     /gene="yebB"
                     /locus_tag="ACHKYO_RS07885"
                     /old_locus_tag="pgaptmp_001577"
     CDS             38102..38704
                     /gene="yebB"
                     /locus_tag="ACHKYO_RS07885"
                     /old_locus_tag="pgaptmp_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HGU4428441.1"
                     /translation="MNINYPAEYEIGYIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(38706..39227)
                     /gene="ruvC"
                     /locus_tag="ACHKYO_RS07890"
                     /old_locus_tag="pgaptmp_001578"
     CDS             complement(38706..39227)
                     /gene="ruvC"
                     /locus_tag="ACHKYO_RS07890"
                     /old_locus_tag="pgaptmp_001578"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HGU4428442.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(39262..40002)
                     /gene="yebC"
                     /locus_tag="ACHKYO_RS07895"
                     /old_locus_tag="pgaptmp_001579"
     CDS             complement(39262..40002)
                     /gene="yebC"
                     /locus_tag="ACHKYO_RS07895"
                     /old_locus_tag="pgaptmp_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4428443.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(40031..40483)
                     /gene="nudB"
                     /locus_tag="ACHKYO_RS07900"
                     /old_locus_tag="pgaptmp_001580"
     CDS             complement(40031..40483)
                     /gene="nudB"
                     /locus_tag="ACHKYO_RS07900"
                     /old_locus_tag="pgaptmp_001580"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HGU4428444.1"
                     /translation="MEDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(40601..42373)
                     /gene="aspS"
                     /locus_tag="ACHKYO_RS07905"
                     /old_locus_tag="pgaptmp_001581"
     CDS             complement(40601..42373)
                     /gene="aspS"
                     /locus_tag="ACHKYO_RS07905"
                     /old_locus_tag="pgaptmp_001581"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGU4428445.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            42683..43249
                     /gene="yecD"
                     /locus_tag="ACHKYO_RS07910"
                     /old_locus_tag="pgaptmp_001582"
     CDS             42683..43249
                     /gene="yecD"
                     /locus_tag="ACHKYO_RS07910"
                     /old_locus_tag="pgaptmp_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU4428446.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            43246..44064
                     /gene="yecE"
                     /locus_tag="ACHKYO_RS07915"
                     /old_locus_tag="pgaptmp_001583"
     CDS             43246..44064
                     /gene="yecE"
                     /locus_tag="ACHKYO_RS07915"
                     /old_locus_tag="pgaptmp_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HGU4428447.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATILHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            44117..44512
                     /gene="yecN"
                     /locus_tag="ACHKYO_RS07920"
                     /old_locus_tag="pgaptmp_001584"
     CDS             44117..44512
                     /gene="yecN"
                     /locus_tag="ACHKYO_RS07920"
                     /old_locus_tag="pgaptmp_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HGU4428448.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            44553..45296
                     /gene="cmoA"
                     /locus_tag="ACHKYO_RS07925"
                     /old_locus_tag="pgaptmp_001585"
     CDS             44553..45296
                     /gene="cmoA"
                     /locus_tag="ACHKYO_RS07925"
                     /old_locus_tag="pgaptmp_001585"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HGU4428449.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLDPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            45293..46264
                     /gene="cmoB"
                     /locus_tag="ACHKYO_RS07930"
                     /old_locus_tag="pgaptmp_001586"
     CDS             45293..46264
                     /gene="cmoB"
                     /locus_tag="ACHKYO_RS07930"
                     /old_locus_tag="pgaptmp_001586"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HGU4428450.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(46429..48858)
                     /gene="torZ"
                     /locus_tag="ACHKYO_RS07935"
                     /old_locus_tag="pgaptmp_001587"
     CDS             complement(46429..48858)
                     /gene="torZ"
                     /locus_tag="ACHKYO_RS07935"
                     /old_locus_tag="pgaptmp_001587"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HGU4428451.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAYPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAIQA"
     gene            complement(48883..49983)
                     /gene="torY"
                     /locus_tag="ACHKYO_RS07940"
                     /old_locus_tag="pgaptmp_001588"
     CDS             complement(48883..49983)
                     /gene="torY"
                     /locus_tag="ACHKYO_RS07940"
                     /old_locus_tag="pgaptmp_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HGU4428452.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT        11914 a        13031 c        12309 g        12904 t
ORIGIN      
        1 tttgaagtca gaccagagtt ttaatttgaa gcagttgcac catggcagtg cgtataatca
       61 ggatgaattg agtaaccctt caacgacggg gttactcaag attgaggaag gattatcgtt
      121 cggcgaccag acgaataata tcttcgtctt caaccgattt ttgactctca acggtctttt
      181 tatctgcttt ctcaggttcg tttgcttcgc acaaacgacg taacagtgca ttttgacgtt
      241 tttgctgatc cagcaacgcc tcaagcagtt caatctgttc gttagtccga gaactggcac
      301 gattgataaa aaaccacaag atgagcccga taagaagaac caccaccgat acaaccaaag
      361 acgcaatgtt catcacgcct gaatttacaa cttcgttcat ttcaccacct ctgagtagag
      421 gcgctattct accactgctg gagaggaaga aaatctagtg ctgaaaaaat gatatcacca
      481 cgggataaac tggttaatgg cacaaattcc gctgaaaaat tgtacatcct gatcgcacat
      541 catgttgaat acctgcgccc aaagcagcaa gcaagccaac accacgacaa ccagaacgac
      601 ccatcgaaac tttttcactc caatctccgc ttcacctacc ttatgtcatt aaactagcat
      661 gcgttagtta aacagcgcct aactatgtcg atattcgtgc tttttcggaa tgagtagctt
      721 gcttcagcgc atattgccgc tacgattaag cgaacataaa aagagaagag gttgtaatgc
      781 gattcatcat tcgcacagtt atgctgatag cactggtatg gataggatta ttactcagcg
      841 gatatggcgt tttgattgga agcaaagaga atgccgcagg attaggtttg caatgtacct
      901 atctgactgc cagaggcacc agcacggtac aatacttgca cactaaaagt ggtttcttgg
      961 ggataacaga ttgcccactg ttacgtaaaa gcaatatcgt ggtcgataat ggttaagggt
     1021 aataaataac cagattctca ctcatgagta aaatattctt atagcaagcg cgtttaatat
     1081 aacgcacctt caactttatt tttctcatgc taaataaaga acctgtctgt ttgatattcc
     1141 gacaggttct tactctcatc agaacggata gtcgtgataa cccatttggg cagaaatttt
     1201 gcgcgctgcg gtatgcaaca ttgcgacata ttcctgtaaa cgctcttcag agaaacgcaa
     1261 cgtcgggaag gagatgctca aaccggcaat gaccacgcca aagcgatcaa ataccggtac
     1321 cgcaatgcat cgcagccctt cttcctgctc ttcgttatct tcgccatacc cctgctcgcg
     1381 cacctggtcc agaacgggta ataacgcttc tgtactggtg atggtccgct cggtactgcg
     1441 tttatactcc acgccctcaa gaatttgctt cacttcatcg agatcgcgcc atgccagcag
     1501 taccttacca atcgcggtgc tgtacagcgg attacgacgc ccaatccgtg aatacatgcg
     1561 caaattgtac atagagtcaa ttttgtgaat gtaaacaata ctgtcttcgt ccagtgcgcc
     1621 gaggtggata gtttctttgg tcaggcggga gatctcacgc atctggatat ctgcgctacg
     1681 aattaaatcg acgttttgta acgcgcgagc gcccagttca aacaatttca gggtcaggga
     1741 atatttctcc gactcccctt cctgcgccac ataacctaag gttttcatgg tctgtaaaaa
     1801 gcgataaacg gtgctttttg acatcatgac gcgctgcgac agctcggtta tccctatttc
     1861 gcgctcttca cccagcgcct gcaaaatgcc aaaaactttt agcacggaag atacagaatc
     1921 aggctgttta tccagatctg cgttagccat ttatcacctc attgcgagtg ttttataaaa
     1981 atcagaactg ttttttatta taatttcgca ccagggtggt cgcaatccat cttttgccgg
     2041 ttagttacaa ttctgcgaca tccaccgtga atatcagtgc tagaatcata cccctgttga
     2101 ttattcacca aagatataaa attcctatgc caaaagttca ggccgacggc ctgccattgc
     2161 cccagcgata cggtgcgata ttaaccattg tgattggtat ttcgatggct gtccttgacg
     2221 gcgcaatcgc caacgtcgcc ctgccaacaa tcgccacgga ccttcatgcc acgccagcca
     2281 gttccatctg ggtagtgaac gcctatcaaa tcgccattgt catctccctg ctctcgtttt
     2341 cgtttctggg cgatatgttt ggctatcgac gtatttataa atgcggtctg gtcgtttttc
     2401 tgttgtcttc actgttctgc gccctttctg attcgctgca aatgctcacc cttgcgcgtg
     2461 tcatacaagg tttcggcggt gcagcgttga tgagcgttaa taccgcactt atccgcctga
     2521 tctatccaca acgttttctg gggagaggga tgggcataaa ctcgtttatt gttgccgtct
     2581 cttctgctgc cgggccgaca attgctgcag caatcctctc catcgcatcc tggaaatggt
     2641 tatttttaat caacgtaccg ttaggtatta tcgccctgct tctggcgatg cgttttctgc
     2701 cacccaatgg ttctcgcgcc agtaaacccc gtttcgacct gcccagcgcc gtgatgaacg
     2761 cgttaacctt cggcctgctt atcactgcgt tgagtggttt cgctcagggg caatcgctga
     2821 cgttaattgc tgcggaactg gtggtaatgg ttgttgttgg tattttcttt attcgccgcc
     2881 agctttctct tcccgtaccg ctgctaccgg tggatttact gcgtatcccg ctgttttcac
     2941 tttctatttg cacatctgtt tgctctttct gcgcacaaat gctggcaatg gtttccctgc
     3001 ccttttacct gcaaaccgtg ctcgggcgta gtgaagtcga aacaggttta cttctgacac
     3061 cgtggccgtt agcaacgatg gtgatggctc cgttggcagg ctatttgatt gaacgcgtac
     3121 atgcaggatt gctgggtgct ctagggttgt tcatcatggc tgcggggctt ttttccctgg
     3181 ttctgctgcc cgcgtcacct gcggatatca atattatctg gccgatgatc ttatgtggcg
     3241 ctggatttgg cttgttccag tcacccaata accacaccat tattacctcc gctcctcgcg
     3301 aacgtagcgg tggagccagt ggcatgttag gaacggctcg tctactgggt cagagtagcg
     3361 gcgcggcact ggtggcgctg atgctaaatc agttcggtga taatggtacg cacgtttcgc
     3421 tgatggctgc ggctattctg gcagtgattg ctgcctgtgt cagtggttta cgtatcactc
     3481 agccacgatc cagggcataa aaaaagcgcg tcgatcagga cgcgcttttt agtatttact
     3541 tcatattact tcaggtattc acccgtacgc agagcttcaa ttcgtttatc cagcggcggg
     3601 tgagtcatga acaactcact gagcgatttc gacttaccgt taatgcagaa agccatcatg
     3661 ctggttgctt cttgcggttc atagctggtt ttcaggcgct gcagcgcggc aatcattttc
     3721 tcgcgaccaa ccagttttgc cgaaccggca tcagcatgga attcacgatg acgcgagaac
     3781 cacatggtga taatgctcgc cagaatacca aacaccagtt ccagaaccgt tgcaaccgca
     3841 aagtagatca gcgggttgcc gttgctctct tcaccttcat cacgatttcc gcccataaaa
     3901 cccgcggcaa gctgcgccag aatacgggaa ataaagataa cgaaggtgtt caccacgccc
     3961 tgaatcagcg tcatggtgac catatcacca ttggcgatgt ggctgatttc gtgagcaatt
     4021 accgcctcgg cttcatccgg gctcatgttc tgcagcaaac cggtgctgac agcaaccaga
     4081 gaggcatcac ggcgcgcacc ggttgcaaaa gcgttgatgt ccggcgcatg gtagatagcc
     4141 acttgcggca tagcgatccc tgcctgacga gcctgggttg ctacagtatt aaccagccaa
     4201 cgttcccttt cgttacgcgg ttgctcgatc acttccccgc caacagatcg taatgccatc
     4261 catttggaca tcagaagcga aacgaaggaa ccaccaaaac cgaacagcaa ggccatgatc
     4321 atcagcccct gaacgctgct cgactgtatc cctgtcaggc tcagtaccag cccgaaaacg
     4381 accattacgg ccaggttcgt tagcaggaag agcgcgattc gcatcataat tttcttttta
     4441 cctcagttta acaaaacgca atatgcgata cccacatcgt atgggttacg cgactatttt
     4501 caagtctgga tagtgcgtaa gtcaccagaa agacacaact ttacattttg tagcatctga
     4561 tttacggcat cttgccgctg ttaaaaaatc aggcacaatt tcttgtgcct gattgatatt
     4621 acttgacggg agcgggttgt tccgcgggtc tggctttttc aagcttcgcc agatcgagtg
     4681 cgatattcac cgtctcatcc agataaggat ccggctcctg gtaatctttc ggtagatcat
     4741 ccagcttctt caactccggt ttaccttcgc gtttaaagcg ttcgttcaaa cgcgccagac
     4801 gcgtcgcatc atcttcatta ttctctttct cacgcacagc gtagttcaga gaaacgatat
     4861 tgcgcttgtc cttcatagcg ttgaagcgcg cgatatcctt catgatgttc tggaactcag
     4921 gatctttcgc gatacgcgca ttatgttcct tcagcagctc cggcccaaag gccgttaaat
     4981 cgcctgattt cacataagtc gcggcatcaa tgctatccca cggcagcgcg ttatcttcga
     5041 atttctcacc cgtttccgtt tcttcattac ccgtcggcat gatgatgtct ggcgttacgc
     5101 ctttacgttg cgtactgccg ccgttaacgc gatagaattt ctggatcgtg tactgcacag
     5161 aacccagcgc tggccattca ggacgtaaca tctgatcgta aatacggttc aatgaacggt
     5221 attgctgaac ggtgccttta ccaaacgtcg gttcacccac aaccagcgca cgaccgtaat
     5281 cctgcattgc cgcggcaaag atttctgaag ccgaagcact gaaacggtca accagtacca
     5341 ccagcgggcc tttatagaac acctgcccgt cggtatcgct gtcttcacga accttgccgt
     5401 tgttatcgcg gacctgaaca atgggacccg caggaataaa cagacctgag agcgataccg
     5461 cttcggtcaa cgccccaccg ccattgctac gcaggtcgat gatcacactg ctgacattct
     5521 gtttttccag tttctgcagt tgaactttga catcgtctgt caaacccaca tagaagcccg
     5581 gaatatccag cacgccgact ttctctttac cgacggtctt caccgacatt ttaaccgcgc
     5641 ggtcttcgag acgaatacgt tcacgggtca atgttacagt gcgggtcttg gtccctttac
     5701 cagcaggtaa aatttccaga cgaactttac tgcccttcgg ccctttaatt aaggcaacca
     5761 catcatcaag acgccagcca atcacgtcaa ccatcggctt gcctgtttga ccaacaccga
     5821 caattttgtc accaacgctg atagctttac tcttcgctgc cggaccacct gccaccatcg
     5881 aattgataac ggtgtagtca tcatccattt gcagcactgc gccaatacct tccagcgaca
     5941 aactcatttc agtgttgaac tgttcggtat tacgcgggga aagatagttg gtatgcgggt
     6001 cgatttcacg cgcaaacgcc gtcattgcca gcgagaaaac atcttcgctg ttggtttgcg
     6061 ccagacgacg aatggcaaat ttgtagcggc gtgtcagggt ttcacgaatt tctttatccg
     6121 tttttcctgc cagcttcagg cttaactcgt cgaatttgac tttactgtcc cacagcgcgt
     6181 tcaactcagc ctcgtttttc ggccagggcg ctttgctgcg gtcaaggtta taagtgtcgt
     6241 tgccggtgaa atccatcggc ttttccagta ccgacaaagc gtactggtaa cgctcaaaac
     6301 ggcgcttttg cgccagattg tagagatcgt agaaaacgtc gagtttgcct gaacgcagtt
     6361 catcgcctaa ctcggttttc tttttcgcga actgttcaac atcgcttgcc agcagcacgt
     6421 tgtggctgta atcgagcaga ttcaggtagc ggtcaaagat tttggccgaa aatgcctgat
     6481 cgaggtcgaa ctggcgataa tgagaacggg tgaagcgcga cgttacgcgc tcacttaccg
     6541 tcgcatgctg cgtctcttcc tttaataccg gaatttgatc agcacgcgtg atatcttcta
     6601 cagcgaaggt ctggcctgct attgcaagca ggccagctaa cgcggtaagc ctaaaaaaca
     6661 tgttcatgcc tggcccggcc tccgtttcag aacaccaggt gttctgcgcg cacaatcaaa
     6721 gacatacccg aattcagctg gacgcggacg ccgtctttgg tgatttctaa tacggtggca
     6781 tccatcgcgt tttgacccgc tttcaccttc agggcttgtc cgacagtcag agctgaaatg
     6841 tcagaaaccg gggtgtgctg ttcttcgcga ggtgctttta ctgtttttgg cgctttctct
     6901 accggctttt gcgcacgagg tttacgttca gcgccttctt tgcggcgtgg cgtggtcgga
     6961 cgtggcttgc gttcgcggcg cggtgcgtct tctttctcac cagcagctgc ggcagcttcg
     7021 cgttttttcg cttgctgttc agcacgctgt gcctgaacac gcgctttcgc ttcttcaagc
     7081 tgcttgcgag catgctctac atgttgctcg tccagctcac cgcatgggtt gccgtcaaga
     7141 tcgacacgcg ttgcgccggg tttaacaccg taaagataac gccagctcga agtgtagaga
     7201 cgtaaagcgg atcgtaattg cgttttgctc aggttcattt ccccggcaac acgatcgacc
     7261 aaatcctgaa aaataccgat tttcagcgga cgcgcttcac cttccgcact gaaacagtgg
     7321 ggaaaacgtt cggccagaaa cgcgattact tctttactgc tattcaactt aggttgattt
     7381 tccatgaaat ttcctgatta caacggacgt agccaacaag cgcaggcatg aacaggcgtc
     7441 attataatga cgctaccagt aaatgctacg ttatccgttg attatcctgc gacgctcgca
     7501 aagaattttt tgtaatccgt cgttgcaagc actttttcaa gctgtgccac aagctgacgt
     7561 aagccttgct cgtcctcgtc tgtaaagcga ccgaagacgg tactatcgat gtcgagaaca
     7621 ccaataatct gatttttcac caccagcggc agaacaattt cagaattact cgccgcatca
     7681 caggcaatat gcccgtcaaa cgcgtgaaca tcctcgatac gctgcacttg attgcgggca
     7741 accgcagtgc cgcacacccc gcgcccgacg ggtatccgga cacaggcaat tttgccctga
     7801 aatggtccga gtaccagtgt atcgtcctca agcaaataaa aacctgccca gtttacgtca
     7861 gtgagacgct cgtataacaa cgcactggtg ttcgcaagcg ttgccagaaa actggtttct
     7921 cctgccatca gcgcgttaaa gtcgcgattt aaatccgcgt aaaattctgt tttgttcatt
     7981 atataatcac ttggttgtct tacctggaac tgccagccta ttaaaataag cattaaatgc
     8041 gttaatgctc aagatcattc ccatcatggg ttaatattaa tgttaattct tattacattt
     8101 ggcacgtcat ggctcttaac acaccacaaa ttacgccgac aaaaaagata acagtgaggt
     8161 caatcggcga ggaacagccg cgtggtgatt accaacgttg cccgcaatgc gacatgctgt
     8221 ttagcctgcc cgagataaat tctcatcaga gtgcctattg tccgcgctgt caggcaaaaa
     8281 ttcgcgacgg gcgcgactgg tcgctaacgc gcctggcggc aatggctttc accatgctgt
     8341 tgctgatgcc gtttgcctgg ggcgaaccgc tgttgcatat ctggctgtta ggtattcgta
     8401 tcgacgccaa cgtcatgcaa ggcatctggc aaatgaccaa acagggcgat acgataacgg
     8461 gggcgatggt ctttttctgc gttattggtg cccccctcat tctggtgtcc tccatagcat
     8521 atttatggtt tggtaaccga ctgggaatga atttacgtcc ggtactgctg atgcttgagc
     8581 gacttaaaga gtgggtaatg ctggatatct acctggtcgg cattggcgtt gcttctataa
     8641 aggtacagga ttatgcccat atccaggcgg gtgtcggctt gttctctttt gtggcgttgg
     8701 tgattttaac gacggtgacg ttgtcacatc ttaatgtcga ggagctgtgg gagcgatttt
     8761 atccgcagcg ccccgctacg cgtagggacg agaaacttcg tgtctgtctt gggtgccatt
     8821 ttaccggcta tcctgatcag cgtggtcgct gcccgcgttg ccatatcccg ctacgcctgc
     8881 gtcgccgtca tagtttgcaa aaatgctggg cggcgctgtt agcgtcaatc gttttgttgt
     8941 tacctgccaa cctgttgcct atttctatca tttatctgaa cggaggacgg caggaagata
     9001 caattctttc cggaattatg tcgctggcaa gtagcaacat tgcggttgcg ggaatcgtgt
     9061 ttatcgccag tattctggta ccgtttacta aagtgatcgt catgttcact ttactgttga
     9121 gcattcattt taaatgccag caaggtttac gcacacgcat tctgttactg cggatggtga
     9181 cctggattgg tcgctggtcg atgctcgacc tgtttgtcat atctttaacc atgtcgctga
     9241 ttaatcgcga tcagatcctc gcttttacta tgggaccggc tgcgttttat ttcggcgcag
     9301 cggtaatttt gactattctt gctgtggaat ggctggacag ccgcttactt tgggatgcac
     9361 atgagtcagg aaacgcccgc ttcgacgact gaagcgcaga ttaaaaataa acgccgtatc
     9421 tcacctttct ggctgctgcc gttcatcgcg ctaatgattg ccggttggct gatttgggac
     9481 agttatcagg accggggtaa taccgtcacc atcgacttta tgtcggcgga tggtattgtc
     9541 ccgggccgta cgcctgttcg ttatcagggc gttgaagtcg gaacagtgca ggatatcagc
     9601 ctcagcgacg atcttcgtaa gattgaagtc aaggtcagca tcaagtccga tatgaaagat
     9661 gcgctgcgcg aagagactca attctggctg gtgacgccaa aagcatcgtt ggcaggtgtc
     9721 tccgggctgg acgccctcgt cggtgggaac tatatcggca tgatgccggg taaaggtaaa
     9781 gagcaggatc actttgtcgc actcgatacc caaccgaaat atcggctgga caatggcgat
     9841 ctgatgatcc acctgcaagc ccccgatctc ggttcgctga acagcggttc attggtctat
     9901 ttccgcaaga tcccggtggg aaaagtctac gactatgcca tcaatcccaa caaacaaggc
     9961 gtggtgattg atgtcctgat cgagcggcgt tttaccgatc tggtgaaaaa aggtagccgt
    10021 ttctggaacg tttccggcgt tgatgccaat gtcagtatca gtggcgcgaa ggtgaaactg
    10081 gaaagcctgg cggcactggt taacggtgcg attgccttcg attcaccaga agagtcgaaa
    10141 cctgccgagg cggaagatac ctttggtctg tatgaagacc tcgcccatag ccagcgtggc
    10201 gtaataataa aactggaact gccgagtggg gccggattaa ccgccgactc gacgccatta
    10261 atgtatcagg gactggaagt cggacagcta actaaactgg atttaaatcc tggtggtaaa
    10321 gtcactgggg aaatgacagt tgatcccagc gtcgttaccc tgcttcgtga aaatacccgc
    10381 atcgaattac gtaatccgaa attatccctt agcgatgcca atctcagcgc cctgctgact
    10441 ggcaaaacct tcgagttggt acccggcgat ggcgagccac gcaaagagtt cgttgttgtg
    10501 ccaggcgaaa aagcactgct gcatgaacct gatgttctga cgctgaccct gaccgcgccg
    10561 gaaagttacg gtattgatgc gggtcagccg ctcattcttc acggcgtgca ggtaggccag
    10621 gttatcgatc gtaaactcac cagcaaaggc gtcaccttta ccgtcgccat cgagcctcag
    10681 catcgagaac tggtaaaagg cgatagcaaa tttgtcgtca acagccgtgt cgacgtgaag
    10741 gtggggctgg atggcgttga gtttctcggt gccagcgcct cagaatggat taacggcggg
    10801 atacgtattc tgccgggcga taaaggcgag atgaaagcca gctatccact gtatgccaat
    10861 ctggaaaaag cgctggagaa cagccttagc gatttaccca ccacaaccgt gagtttgagt
    10921 gcagagacgc tgccggatgt gcaggcagga tcggtagtgc tctaccgtaa atttgaagtt
    10981 ggtgaagtta ttaccgtccg tccgcgagct aacgcgtttg atatcgatct gcatattaag
    11041 ccggagtatc gcaaccttct gaccagcaat agcgtgttct gggcagaagg cggggcgaaa
    11101 gttcagctga atggtagtgg cctgaccgta caggcatccc cgctctccag agcattaaag
    11161 ggagccatta gcttcgataa cctcagcggt gccagcgcca gtcagcgtaa aggcgacaaa
    11221 cgaattctgt atgcttccga aacagcggcc cgtgcggttg gtgggcagat tacgcttcac
    11281 gctttcgatg ccggaaaact ggcggtcggg atgccaattc gctatctcgg tattgatatc
    11341 gggcaaatcc agacgctgga tctgattacc gcgcgcaatg aagtacaggc aaaggcggtg
    11401 ctctatccgg agtatgtcca gaccttcgcc cgcggcggta cgcgcttctc ggtggtcaca
    11461 ccgcaaattt cagccgcggg cgttgagcat ctagatacta tcctccagcc gtatatcaac
    11521 gtcgaaccag gccggggcaa tcctcgccgc gactttgaat tacaagaggc caccattact
    11581 gattcgcgtt acctggatgg cttaagcatt attgttgaag cgccggaagc cggttcgtta
    11641 ggtattggta cgcctgtgct gttccgtggt ctggaagtcg gtacggttac aggaatgacg
    11701 ctggggacat tgtcagatcg cgtgatgatt gcgatgcgca tcagtaaacg ctatcaacac
    11761 ctggtgcgta acaattccgt cttctggttg gcatcgggtt acagtctgga ctttggtctg
    11821 acgggcggcg tagtgaaaac cggcaccttt aaccagttta tccgtggcgg catcgccttc
    11881 gccacgcctc cgggtacgcc actggcaccg aaagcccagg aaggcaaaca cttcctgttg
    11941 caggaaagtg aaccgaaaga gtggcgtgaa tggggaactg cgcttcccaa ataatgccca
    12001 ctgctccggc gtgcctgcgc cggagcgttt atgctaaact gcgcgcctgt ttttttgcca
    12061 gtggtacatg ctcgtggccc aacacaccgt ttatttcccg gacgcctttc tgacacaaat
    12121 gcgcgaagcg atgccttcga cgctctcatt tgatgatttt cttgccgcct gtcagcgccc
    12181 gttgcgccgc agcattcgcg ttaatacgct gaaaatctcc gttgctgatt tcctgcaatt
    12241 aaccgctcct tatggctgga cgcttacgcc aattccgtgg tgtgaagaag gtttctggat
    12301 tgaacgcgac aatgaagatg cattgccatt gggtagtacc gccgagcatt taagcggcct
    12361 gttttatatt caggaagcca gttcaatgtt gcccgttgcc gccttgtttg ctgacgataa
    12421 tgcaccacag cgggtgatgg atgtcgcagc tgcgccaggc tccaaaacga cgcaaattgc
    12481 cgcgcggatg aataacgaag gggcaatcct tgccaatgag ttttccgcca gtcgggtaaa
    12541 agtgttacat gccaatatca gccgctgtgg catcagtaat gttgcgctca cacattttga
    12601 tggccgcgtg tttggtgcgg cagtgccaga aatgttcgat gccattttgc tggacgctcc
    12661 ctgctctggc gaaggcgtgg tgcgtaaaga tcccgatgcg ctaaaaaact ggtcaccaga
    12721 aagcaatcag gaaatcgcag ctacacaacg ggagcttatc gacagcgcct ttcatgcatt
    12781 acgtcctggt ggtacgctgg tttactcgac ctgtacctta aaccaggaag aaaacgaagc
    12841 cgtttgcctg tggctgaaag agacttaccc cgacgcagta gagtttttac cacttggcga
    12901 tctcttccct ggtgcaaaca aagcgctgac cgaagaaggc tttttgcatg ttttcccaca
    12961 aatttacgac tgcgaaggct tcttcgttgc tcgtctgcgt aaaactcagg cgattcccgc
    13021 cttacccgcc cccaaataca aagtgggcaa tttcccgttc agcccggtga aagatcgcga
    13081 agctggacaa attcgtcagg cggctgcagg tgttggctta aactgggatg aaaacctgcg
    13141 cctctggcag cgtgacaaag aactgtggtt gttcccggtg ggcattgaag ccctgatcgg
    13201 taaagtccga ttttctcggt tggggattaa acttgccgag acgcacaaca aaggttatcg
    13261 ctggcagcat gaagcggtta ttgctcttgc ctcacccgat aatatgaatg cattcgagct
    13321 gacaccgcag gaagcggagg agtggtatcg cgggcgcgat gtttacccgc aagccgcgcc
    13381 agtggcggat gatgtattgg ttactttcca gcatcaaccg attggtttag ccaaacggat
    13441 tggttcgcga ttgaaaaaca gctatccgcg tgaactggtg cgcgatggga aactttttac
    13501 cggtaacgcc tgacagcgca caaaaaaagc gcactttttg actggcacat tcggctgcct
    13561 caactaggct gaaaaatggt gcgatcggac tggtcgtacc acaatcggca gctaaatgga
    13621 gagcacgaag atgaaaacca gtgtgcgcat aggcgctttt gaaatcgacg acggcgaatt
    13681 acacggtgaa tcgccaggtg atcgaacgtt aaccattcct tgtaaatctg accccgattt
    13741 atgtatgcaa ctggatgcct gggatgctga aaccagtatc cctgccctgc taaatggcga
    13801 acactctgtc ctttaccgta cccgttacga tcaacaatct gatgcctgga ttatgcgtct
    13861 tgcctgatcc aaaaagaacc cgtcggcatg gcgggttatt tgtcctggtt attcccccgt
    13921 tgtaaaatct ctcctaaact taacggtacg gcaccacact tcggggatga aatgttcgcg
    13981 ctggtacttt ttgtttgcta cctggatggc ggttgtgaag atattgttgt ggatgtctac
    14041 aacacggaac agcagtgtct ttattctatg agcgatcaac ggatccgcca tggcggttgt
    14101 tttccgattg aggattttat agatggtttc tggcgaccag cacaggagta tggtgatttt
    14161 taattattgc aattgcacaa gagtcagttc gcccccaaag acagcaccgg tatcaatata
    14221 atgcaggttg ccaatatcca cgcgatgtcg caacggtgta tgaccaaacc agaaatgatc
    14281 agcacctgta attccctgcc ctttttggcg ttcacctaat cgcgagcggc tccacaagac
    14341 ctgatgcaaa tcaacgtcct tttgccattc ataaacatca tctggataat cggcatgagc
    14401 aataacatgt ttgccggtac gactgtgtac ttcaagaata aagggcaaat gctgacattt
    14461 ttccagcgcc gttttcgctt gtttctgttg attatctgcc agcgcaataa accagtcgcc
    14521 gccattcatc aaccacaaag acatctgctg ggatgccagc gcatccatcg ccatctgttc
    14581 atgattgcct cttaccgcac aaacccaatg ttgttccagt aactgcagac aacgtaaact
    14641 ttgcggccca cgatcgataa cgtctcctac tgagataagt aaatctcgcc acggatcaaa
    14701 acgacaatgc cataatttgc ggcgcaactg ctcaagacaa ccgtgtatat cgccagaaag
    14761 ccagatatgt cgccattgat gacccgcaat tctctgataa acgggcgcag gctgtttcat
    14821 caatattttc ctcccgcgct aaagatcaca taatcttaac aagaatgtta aaaaacgctg
    14881 gactcagaca gtagagtgtg tgttatggtt gactataaag tcagcgaagg aaatgcttct
    14941 ggcttttaac agataaaaag agaccgaaca cgattcctgt attcggtcca gggaaatggc
    15001 tcttgggaga gagccgtgcg ctaaaagttg gcattaatgc aggcttagtt gccttgccct
    15061 ttaagaatag atgacgacgc caggttttcc agtttgcgtg caaaatggtc aataaaaagc
    15121 gtggtggtca tcagctgaaa tgttaaaaac cgcccgttct ggtgaaagaa ctgaggcggt
    15181 ttttttattg gaaatcaaaa ggctatttta ggtaattaac agagtttttc agctcgttct
    15241 ataaacggtg ccagactcat tttttcgccg ggattgttag gatcatcaat ctgaatcacc
    15301 gaaatgggtt gggctttagt cttcccactg gcaacttcct tttgtgcgat atcgtttaaa
    15361 ggatactgca cgagggtact tgggttaatg acatacaaag cattacccgg tctgcaagtc
    15421 agcatcacct cttcgcgatt aaacgcccat ttgtctttac ccacttcaaa acgactgacg
    15481 gtaatcacct gcggtgcagc cagcgccgct gcagaactgg tgagtaacag aaacgtcaga
    15541 atactttttt tcatcatttt cgtaatccat caaaaaggtt cgagagtcgc aagcaggctg
    15601 atgatcacca gcactaccgc acctatcgcc cattcgagtt tagtcatcca ctcaaaccag
    15661 ggtgccgcac gatcttcatc ttgcctcatg cgcggtacaa gaacataacg attagccagc
    15721 gcaatgacca ccataatcat caccaggatg gctttgagca ataagagttg gccccagtaa
    15781 gtcgtaagcg tggggggaaa tccggtaatc aacaatgcat taagcacgcc gcttgccagt
    15841 acgccgatca ccgcaaaatg cccgcaccag gaaaaacgca tcagcgcctg aatggcctgg
    15901 tgtcgccaac ggcctttgat gagttgcata caccagagca ccggcagtaa tcccccaaac
    15961 cacgccgccg cacaaatcag gtgaatcgcg tgattagtct gatggatttt cgccgttacc
    16021 ccttcattca tcgtcgcatg ccccactccc gccagcagaa taaattgcgc ggtagtgagc
    16081 ataaacagca aacgtgacac attccgcggt tgcataaggg cgacgatcaa cgttacgagg
    16141 gcgagaacaa tttgccataa ccagacgcca ccaaactgcg tttgcaaaac agcctgccaa
    16201 atgtttggcg aaaatacatc tgcccatccc gttcccatca acccgccctg aacggcgagc
    16261 attgcagtgg cactgatcaa actccagacg gcagcgtgct gttgtaaacg cagaaaacgc
    16321 ttcgccagca agcgacgaat cgttaagggg gccagccagg caccgtacat tgcgaagcca
    16381 aagaccagca tcagcgaggt gaaatggata aatcgcagcg cgatccaggt aaacgccagc
    16441 atgatttatt tcacgctaaa ggtgtagtgt cctttcgttt tatgcccgtc caccgaaaca
    16501 acatgccagt caacgatgta ggttccgggt ttcagcgaat cggccagtgg aacaatcagt
    16561 tgcttttggt cctgctcatt tcgcttcgcc ggtaatgttt taatattttc gttttttggc
    16621 cccgtgattt ttgcaccact gaatcctgtt tcaacacctt ccgagaagtt taaagtgatt
    16681 gcctgcggtg cagctgtcac ttgcgcgttt gccgcaggat actgatgcgt taaatgcgca
    16741 tgtgcccaga cggaaggtgt caccaacgaa gtggtcagga ttgccagcgc gtagcgaagg
    16801 gagcgtgcag ttgaagtcat attatctatt cctttttgta ataacttttt tacagagcat
    16861 aaccttgtct aatgtctgag tcgaggatca tcaattccgg cttgccatcc tggctcactc
    16921 ttagtaactt ttgcccgcga atgatgagga gattaagaat gctgaagaat ctggctaaac
    16981 tggatcaaac agaaatggat aaagtgaatg tcgatttggc ggcggccggg gtggcattta
    17041 aagaacgcta caatatgccg gtgatcgctg aagcggttga acgtgaacag cctgaacatt
    17101 tgcgcagctg gtttcgcgag cggcttattg cccaccgttt ggcttcggtc aatctgtcac
    17161 gtttacctta cgagcccaaa cttaaataaa acttatacag agttacactt tcttacataa
    17221 cgcctgctaa attatgagta ttttctaaac cgcacccata atttgcagtc attttgaaaa
    17281 ggaagtcatt atgtcgttct ggaaagttgc agcagcgcaa tatgagcctc gtaaaacctc
    17341 actcaccgaa caggtagctc atcatctgga gtttgtgagg gccgccgcca ggcaacaatg
    17401 tcagctgtta gtttttccat cgctttcttt actggggtgt gattattcgc gacgtgccct
    17461 tcctgcccca cccgatctct cactgttaga cccgctttgt tatgccgcaa cgacctggcg
    17521 aatgaccatc attgccggcc ttcccgttga atataacgat cgctttattc gtggaattgc
    17581 ggtgttcgca ccgtggcgaa aaacgcctgg gatctaccat cagagtcatg gtgcatgtct
    17641 gggtcgtcgt tccagaacca tcacggtggt cgatgaacaa ccgcaaggta tggatatgga
    17701 cccgacctgt tcactgttta ctacagggca atgtctggga gaacccgacc tgctggcgtc
    17761 ggcccgtcgc ttacaatttt tttcacatca gtactcgatt gccgtgctga tggcaaatgc
    17821 ccgtggtaac agtgcactat gggatgaata tggtcgtctc atcgttcgcg ccgatcgtgg
    17881 ttcattattg ttagtcggtc agcgttcatc acagggttgg caaggcgata tcattccatt
    17941 acgctaggct ttttcggcct ggagcatgcc atgttgcgca ttatcgatac agaaacctgc
    18001 ggtttgcagg gagggatcgt tgagattgcc tctgttgatg tcattgacgg aaaaatcgtc
    18061 aaccccatga gccacctggt gcgccccgat cgtcctatta gtccacaagc gatggcgatt
    18121 catcgcatca ccgaagccat ggtcgccgat aaaccgtgga ttgaagatgt gatcccacac
    18181 tattacggta gtgaatggta tgtcgcgcat aacgccagct ttgaccgccg cgtactgcct
    18241 gagatgcccg gtgagtggat ttgcactatg aaactggccc gtcgtttgtg gcctgggatc
    18301 aagtacagca atatggcgtt atataaaaca cgcaagctca atgtacagac gccgccgggc
    18361 ctgcatcatc accgcgcgtt gtatgactgt tatatcaccg ccgcgttgct tatcgatatt
    18421 atgaacacct ccggctggac ggcagaacag atggccgata tcaccggacg tccgtcgttg
    18481 atgacgacct tcacctttgg caaataccgt ggcaaagcgg tttccgacgt tgccgaacgc
    18541 gatccgggct atctgcgctg gttatttaat aacctggaca gcatgagccc ggagctgcgt
    18601 ttaacactga aacattatct ggaaaatact tagtccgcag gcgtagcggg taatgttccc
    18661 tgcgccagcg cgaccagaaa agcatattcc atcgctacgc cttcgtacga tttaaagcga
    18721 ccagatttac cgccatggcc tgagtccatg tcggtacaga gcaataaaag atggtcatcg
    18781 gttttcagct cgcgcaattt agcgacccat tttgccggtt cccaatattg cacctgagaa
    18841 tcgtgcaaac cggtcgttac cagtaaatgc ggataagcct gtgcggtgac gttgtcatac
    18901 gggctgtagc ttttcatgta ctcgtaatat tgcggatcct gcgggttacc ccactcttca
    18961 aactcaccag tggtaagagg aattgattca tcaagcatcg ttgttacaac atcaacaaac
    19021 ggtacctggg cgataacgcc gtggaataat tccgggcgtt gattaattgc aacgcccatc
    19081 aacatgcccc ccgcactccc gcccatcgca taacaaagcg aaggagagcc atagcccagt
    19141 tttaacaatg catcgcaggc atcaagataa tcattaaacg tatttttctt cttcagaaat
    19201 tttccgcctt cgtaccattg ttgccccagc tcaccaccgc cgcgaacatg gacaatggcg
    19261 tagacaaagc cacgatctaa caaactcaag cggctaaaac tgaaatcggc atcaatactt
    19321 gcgccgtaag aaccatagcc atacaccagc aacgggttgt gtcctttgcg aaaatgtttg
    19381 cgatggtaga ccaacgaaac cggaacttcg acgccatcac gggcgactat ccacaggtgt
    19441 tcactgcggt aattcgccgc ataaaaacca ggaacttccg tttgttttaa tacacgacgc
    19501 tcaccggtat ccatatccag ttcaaacaaa gtgtctggtg tagtcatgga agaataacca
    19561 taacgcaatc gcgcggtttc aggttctgga ttgtaggcaa tccaggtcac ataggccgga
    19621 tcatcaaagg caataccaat gacttcccgg gtcttgcggt taatttggcg caaactggtt
    19681 aacccgcgct gacgctcttc aaccaccagc cagtcggtaa acagcgtaaa cccttccagc
    19741 atgatgtttt cgcgtggcgg aattaactct tcccactgtt gctcatcacg catacgggta
    19801 cggtataagc caaagttttt gccgtggcgg ttggaacgca gataaaaacg atgctggtag
    19861 tgatcaaggc tgtattcgtg atctttgcgg cgcggcagaa aaacaaacgg ctcggcatcg
    19921 gccatttccg cgtccagcag gcgaacttca ctggtggtgg cgctggccaa atgaatgact
    19981 acatagtgct tcgaggtcgt tttatgcagg ctgacgtaat aggtatcgtc tttttcttcg
    20041 tagatcagtt tatcatgcga tgctggcgta ccgatggcgt gacgccagac ctgataaggc
    20101 agcagcgtca ccggatgctt gcgaacatag tagaaagtcc aggagtcatt tgcccagaca
    20161 aagctgggtt caacgttatc cagcagttcc gggtaccagt taccagtttc cagattacga
    20221 aaacgaatgc cgtactggcg tcgggaaaga aaatcttctg ccagcgccat aatggtgtta
    20281 tcgggcgtaa tcgccattcc gcccatcgaa taaaactcac tatgagctgc gcgcttattg
    20341 gcatcgagca atgtttccca ctcatcccac tcttcactga atgccgattg acgctggtag
    20401 atagcatatt cacagcctgg ttcataaata tgccgatagc ggtagccatt tttgatgtag
    20461 ggcgcagaaa cttctcgttg cggaatgcgg tcgatgattt cctttaagat gcgatcctgc
    20521 aaggcttgtt gtgaggccat cacccgatga ccgtaactat tttcttgttg caggtagtcc
    20581 aggacttctg gctgagaacg cgtatcgtcc cgcagccagt agtaattatc gatgcgcgta
    20641 tcgccatgaa gcgtcatggc gtggggaatg cgggcggctt ttggtagcat gttattgttc
    20701 tttctggttg aaacatctta taagggtggc aaaactcacc ggggatgcaa gcgaaacagg
    20761 ggagtcattg cttagatgat gacaggtaat ggcgcggata tcgaatgtta tgcagacaga
    20821 gaaaatcagc ctgttcaaat ggctgtgcga ttctggatag cccgaaatag tcaacttcag
    20881 gctatccaga gagcggaatt attccgccaa agtgcgtttt tgctgttcga gatcgcgttc
    20941 aatgccgtca cgaacatcct gggggatttt cagcgcgtca cccagtgcat tcaggtaact
    21001 gcgttccata aaatggtcaa tatcaatagc cgcgcaactc aggaaataga tttccagcgc
    21061 ctcttcttca ttgcggaccc cggtagccag gcgttgtgga tccagcggtt gttcgattgc
    21121 ctgctcaatg agtacacgcc cctgctcttc cacgccggct tcacgcaatt gctggtcgat
    21181 tgccgcacgt tctttggcat caatatgacc atcactttta gcggcaaaga ccagcgcaag
    21241 gatcaaacgt tctgtacgct catccagcgg cgtactttgc gcgccaaact gcggttcgtc
    21301 ctgatgcgcc gcgcgaattt tatctttgta tttattccac agcaccgtac ccgctaccgc
    21361 tccgccgcca accagtaacg cgttggtgcc atatttcgta agaagtttac gtgctgattt
    21421 atttgcgacc agcagcccag ccagtccgcc taatgcgcct ggcactaaca gtttgcccaa
    21481 tccctgatcc gcagacgagg aggtagaaga actgctttgc ccaagcaggg attgcagttg
    21541 atttaaccag ttagccatat ttgctcctca ataaccattt ttagtgcctg tagcatagcg
    21601 gatgaggatg tcagaaaatg tatatcgaga caaaagatgc gcaaataagc ggggcggtag
    21661 gccgttattc aaagaaattc gcgccaggta aggtatttac tgtatatcag gtgataaggg
    21721 tattttaggt gaaagtagtt tgtcgttttg agtggcattg tttgatgctg attgcccgat
    21781 gcgacgctgt tgcgtcttct caggcctacg attgtggtgc gttctggggc ggacaaggca
    21841 ctcgcgccgc atccgccaac ggtcaatcga attatcagaa gccattaacg ccgctgatat
    21901 tccgccattc ccgctttgca gtccacgctg acctggtaat gaatatcggc acttttacca
    21961 cgcacggtta gcggtactga ccatttatca tctttaccct gaatgtcctg caaactgacc
    22021 caagccacgg gatcggcctg accgacaatt ttttgatcat ctgcccagcg cgccacgcga
    22081 ttttgttgat aatcacgttt tacgctcgcg gcaattccgg cagcatccag accttcacac
    22141 tttgggaaag tgaccgactt gctggtttca ttattggcag cgaaaactga tgcgcaggca
    22201 gaaaccaaca acagccctaa aaacgcccct ctttttttca tgtttttctc catagcacaa
    22261 tgattcagga gaaagcatgg tacaaattgt caggagcgca agttgcttca ggccgcgtgt
    22321 gaggcatctt cctcttcgga ttcagcaccg acgacctgtg gggacggtaa tttgccggtt
    22381 ttcagaatgg tgctcagcac atctttttgt tctgccagcc atagcgaaag ggcttcacgt
    22441 tgctcgtctt ccatttgcac tggagaattt gtcagccagg tgtcaagcag atccgccgta
    22501 tcaagcattt tatcataagc atcggcttcc tttttgctgg taaacgacat tttctcttcg
    22561 ccctcacgaa tgactacgta tttaacttca accgccattt gcagcctctc ataataactg
    22621 tgattttata cagtatattt cttttcggtt gagaaatcaa catcagcaat aaagacacac
    22681 gcaaacgttt tcgtttatac tgcgcgcgga attaatcagg ggatattcgt tatgacgtta
    22741 ttaggcactg cgctgcgtcc ggcagcaact cgcgtgatgt tattaggctc cggtgaactg
    22801 ggtaaagaag tggcaatcga gtgtcagcgt ctcggcgtag aggtgattgc cgtcgatcgc
    22861 tatgccgacg caccagccat gcatgtcgcg catcgctccc atgtcattaa tatgcttgat
    22921 ggtgatgcat tacgccgtgt ggttgaactg gaaaaaccac attatatcgt gccggagatc
    22981 gaagctattg ccaccgatat gctgatccaa cttgaagagg aaggactgaa tgttgtcccc
    23041 tgcgctcgcg caacgaaatt aacgatgaat cgcgagggta tccgtcgcct ggcggcagaa
    23101 gagctgcagc tgcccacttc cacttatcgt tttgccgata gcgaaagcct tttccgcgag
    23161 gcggttgccg ccattggcta tccctgcatt gtaaaaccgg tgatgagctc ttccggcaag
    23221 gggcagacgt ttattcgttc tgcagagcaa cttgctcagg catgggaata cgctcagcaa
    23281 ggcggtcgcg ccggagcggg ccgcgtaatt gttgaaggcg tcgttaagtt tgacttcgaa
    23341 attaccctgc taaccgtcag cgcggtggat ggcgtccatt tctgtgcacc agtaggtcat
    23401 cgccaggaag atggcgacta ccgtgaatcc tggcaaccac agcaaatgag cccgcttgcc
    23461 cttgaacgtg cgcaggagat tgcccgtaaa gtggtgctgg cactgggcgg ttatggtctg
    23521 tttggtgtcg agctgtttgt ctgtggtgat gaggtgattt tcagtgaggt ctcccctcgt
    23581 ccacatgata ccgggatggt gacgttaatt tctcaagatc tctcagagtt tgccctgcat
    23641 gtacgtgcct tcctcggact tcctgttggc gggatccgtc agtatggtcc tgcagcttct
    23701 gccgttattc tgccacaact gaccagtcag aatgtcacgt ttgataatgt gcagaatgcc
    23761 gtaggcgcag atttgcagat tcgtttattt ggtaagccgg aaattgatgg cagccgtcgt
    23821 ctgggtgttg cgctggcgac cgctgagagt gttgttgacg ccattgaacg cgcgaagcac
    23881 gccgccggac aggtaaaagt acagggttaa acccggcaaa aaaacgctac aaaaatgccc
    23941 gatcgtagat cgggcgtttt gacttttaca gcttagcgcc ttctacagct tcacgcgcca
    24001 gcttagtaat gcggtcgtaa tcgcccgctt ccagcgcatc tgccggaacc agccaggaac
    24061 caccgatgca cagcacgctt ttcagcgcca ggtagtcacg gtagttagcc ggagaaatac
    24121 cacccgtcgg gcagaaacgg acctgggaga acggacccgc gatcgcctgc agggctttca
    24181 cgccgccgtt agcttcagcc gggaagaatt tgaactcttt caaaccgtag tccataccca
    24241 gcatcagttc ggaaacagtg ctgatccccg gaattagagg aatagtccct tcggtagcag
    24301 ctttcagcag cggctcggtc agacccgggc taattgcgaa ctgtgcaccc gcttcagtga
    24361 cttctgccag ctgctgtgga ttcagcaccg taccggcacc cacaatcgct tcaggcactt
    24421 ctttggcgat agcacggata gcgtcaactg cacactcggt acgcagagtc acttccagaa
    24481 cgcgcacccc accagcaacc aacgcttttg ccatcggcac tgcgtgttcc agttttttta
    24541 ccacgataac cggtacaacc gggccggtgg tcaggattga ttctgcactt gttttccagt
    24601 ttttcatcag agttttctct cgcctgatta caaatttgtc gtcttaaaaa gtgatacagg
    24661 ttgcgccctg ttcggcaccg gacagttttt cacgcaaggc gctgaataat tcacgtcctg
    24721 tccccacgcg tgacgcgctc aggtcaggaa tgcgcggttc gcgagcagcc agttccgctt
    24781 cgtctaccag cagcgtcagt tcgcctgtct gtccattcac acgaatgatg tccccgtcgc
    24841 gcacttttgc cagcagcccg ccatcgtagg cttctggtgt tacgtggata gctgacggca
    24901 ctttacctga agcgccggag agtcgtccat cggtaactaa cgcaattttg aaacaccggt
    24961 ccaataatac accaagtggc ggcatgagtt tatgtaattc tggcattccg ttcgcttttg
    25021 gcccctgatg acggacaaca acgacacaat cgcggtccag caaacccgct tcaaaggccg
    25081 gcataacgtc atgctggctt tcaaaaacaa ccgctggcgc ttcaatcacc tggttctcaa
    25141 ccggcacggc agaggttttc ataaccgcac ggcccaggtt accgcttaac acttttgtcc
    25201 caccatgatg agagaaaggt tgttcgaagg aagcgatcac attgctgtcg agtgattttt
    25261 ccgccccttc ccgccagtcc agttcaccat tattcagcca tggttcaagg gtataacgag
    25321 acagaccaaa gcctgccacc gtattgacat cttcatgcag caggcctgct ttgagcagtt
    25381 cacgcaccag aaccggtacg ccacctgccg cctggaagtg gttaatatcg gccggaccgt
    25441 tcgggtagag acgtgccatc agcggtacaa catcagaaag gtcagagaag tcatcccagt
    25501 taatctgaat accggccgcg cgcgccatcg ccaccaggtg catggtgtgg ttagtggaac
    25561 caccggtcgc cagcagtgca acgataccgt tcaccaccac tttctcatcg atcatcttac
    25621 cgatcggcat ccattcatta ccattaccgg tcatgcgtgt aacctgacgc gcagcggcag
    25681 cggtcaaagc atcgcgcagc ggagaatccg gatgaacaaa agaagagcct ggcaactgca
    25741 tccccataaa ttccaccacc atctggttgg tgttggcagt accgtagaaa gtacatgttc
    25801 ccggcgcatg gtaagacgcg gcttctgact ccagtaaggc catgcggtcc actttacctt
    25861 cggcataaag ctggcgaata cgcacttttt ctttatttgg caaaccgctt gccatcggtc
    25921 cagacggcac aaacaccgca ggcaaatgac caaacgacag ggctgccatc gtcagacccg
    25981 ggacaatctt gtcgcacaca ccgaggaaca gagcaccatc aaacatgtta tgggacagcc
    26041 ccaccgccgc agacatcgct atcacttcgc ggcttagcag cgacaattcc attccatcct
    26101 gcccctgggt gacaccatca cacatcgccg gaacaccgcc cgcaacctga ccaaccgcat
    26161 tcgcttcatg cagggcttta cgaatgattt ctggatagtg ttcataaggc tggtgcgcgg
    26221 agagcatgtc gttataggag gtgatgatgg cgatattgtt acgcaacatg cttttcaaag
    26281 aggctttgtc ttctggctgg caggcagcga aaccgtgtgc caggttaccg catgccaact
    26341 gcgaacgatg aacggtcgac gttttcgctt gttctatccg ggcgagataa gcagagcgag
    26401 tctcgcgcga acgttcaatg attcgatttg ttacgcgtaa caattgtgga ttcataaagg
    26461 ctcctgaaat tgagttgtca gagcaggatg attcacaacg cgtttcattc agaggattta
    26521 tgagtgaaac gcctgtaacc ggagctcata gggcaaaaac gtttcagtca gtgtaataaa
    26581 aaaagcctcg tgggtgaatc cgcacgaggc ctgaaagtgt aaaaattgtt ctacaatctg
    26641 tgcaagatca tgttaccggt aaaataacca taaaggataa gcgcagatat tactcaaact
    26701 cattccagga acgaccatca cgggtaatca tcgccaccga ggcaacgggt ccccaggttc
    26761 cggcctgata cggtttcggc gcatcattgt ccatcgccca cgcctcagta atggagtcta
    26821 cccatttcca ggcttcttcc acttcgtcgc gacgaacaaa cagtgcctga ataccacgca
    26881 tggtttccag cagcaaacgt tcataggcat ccgccagatg cgtctgatta aaggtttctg
    26941 aatagctcag atccagcttg gtgatttgca ggttatgttt gtggtcaagg ccaggaactt
    27001 tattcagtac ctggatatcc acgccttcat caggttgcag acggatagtc agtttattct
    27061 gcggcagatc ctgccacgac tctttaaaca gattcagttc aggtgttttg aaatagacca
    27121 cgacttcaga acatttggtc ggcagacgtt taccagtacg caggtagaat ggcacaccgg
    27181 cccagcgcca gttatcaatg tcgacgcgga tcgccacgaa ggtttctgta ttgctgctct
    27241 tgttcgcgcc ctcttcttcc agatatcccg gcactttttt gccctgggcg aagcccgcag
    27301 tatattgccc gcgtacggtt ttttcgcgta cgttggagcg gtcgatgcgg cgcagagact
    27361 tcagtacttt cactttttca tcgcggatgc tgtctgcgct caggtcagac ggcggagaca
    27421 tcgcaatcat gcaaagaatt tgcagcaggt ggttctggat catgtcgcgc atctgaccgg
    27481 ctttatcaaa atagccccag cgcccttcga tccccacttc ttctgccacg gtaatctcaa
    27541 catgatcaat ggtgcgattg tcccagttat tcacaaacag ggagttagca aaacgcagcg
    27601 ccaacaggtt cagcaccgtt tctttaccaa gatagtggtc gatacggtaa acctggcact
    27661 cctcgaagta ttcgccaacc tgatcattga tttcctgcga ggtcgccagc gacgtcccca
    27721 gcggtttctc catgactacg cgtgccggtt tagcattcag ttttgcctcg ccaagccctt
    27781 tgcaaattgc gccaaaagtg ctgggcggca tggcaaagta gttaatggtg atacgatttt
    27841 tttgatccag catcgcgccg agacggctga atgcagcagt gtcattgaca tcgagattac
    27901 aaaaatccag acgtgcactc agggtgtccc ataaaccttc atcaatggtt tctttcatga
    27961 aagtttcgag cgcctcgcgg acaactttgg tatatgccgc tttatcccag tcagcacgcc
    28021 ctacgccgat aatccgggtg tccgggttga gctgaccggc tttttccagt tgatacaggg
    28081 aaggcagcaa tttacgacgc gcaaggtcgc ctttcgcgcc gaaaatgacc aggtcacagg
    28141 cctgggctgt ttgcgttacc gccatgtcat tctccttaag ttaactaacc cggtacttaa
    28201 gccagggtat acttgtaatt ttcttacggt gcactgtact gcttttacga gcttgcgaaa
    28261 actgtaaacg cttatccacc cgtgcgatta cgggaaaagc gcgcaaagtg cggcaaaact
    28321 gataaaaaaa tcatcgtttt ccattgggta aaaatctgac actgatcatg ttatgaaaaa
    28381 aaataacaac ttttttatct gcttttgtca ttaacacggc acacaggcgt aatatctgac
    28441 aaaactgcat ttcgatttct ttcagtgcgg aaatcgtcat tacccgtgag tctctttaca
    28501 tcatgaatat gctggaaaaa atccagtctc agctggaaca tttgagcaaa tcagagcgca
    28561 aagttgccga ggtcattctg gcttcgcccg ataacgcgat ccattcgagt attgctgcta
    28621 tggcactgga agccaatgtt agcgaaccga cggtgaatcg tttctgtcgc agcatggaca
    28681 cgcgcggttt tcctgatttt aaacttcatc tggcacagag tctggcgaat ggcactccct
    28741 atgttaatcg caatgtcaat gaagatgaca gcgttgaatc atacacaggg aaaatatttg
    28801 agtccgcaat ggcaacgctt gatcatgtcc gtcattcact ggataaatct gccatcaacc
    28861 gcgccgtcga cttgctcact caggcaaaaa aaatcgcctt tttcggatta ggctcttcag
    28921 ccgccgttgc ccacgatgcg atgaataagt tctttcgttt taatgttccg gtggtgtact
    28981 ccgatgatat cgtgctgcaa cgcatgagtt gtatgaattg tagcgacgga gacgtggtgg
    29041 tgctgatttc tcacactgga agaacaaaaa atctggtcga gctggcgcag ctggctcgcg
    29101 aaaacgacgc catggtgatt gccctcacct ctgcgggtac cccgctcgcc cgggaagcaa
    29161 cgctggcaat taccctcgac gtaccggaag atactgacat ttatatgccc atggtttctc
    29221 gacttgcaca gctgaccgtg atagatgtgc tggcgacagg atttactttg cgacgcggtg
    29281 caaaattcag agataacttg aagcgggtca aggaagcgct gaaggaatcg cgttttgata
    29341 agcagttact taatttaagt gacgatcgct aaaaacgact gtcactgtcc taatcttata
    29401 cgacatccga atgagattaa tttatcgcca tcgcggcgtt atttcattcg gatttcatgt
    29461 tcaagcaaca cctggttgtt tcagtcaacg gagtattaca tgtccagaag gcttcgcaga
    29521 acaaaaatcg ttaccacgtt aggcccagca acagatcgcg ataataacct tgaaaaagtt
    29581 atcgcggcag gtgccaacgt tgtacgtatg aacttttctc acggctcgcc tgaagatcac
    29641 aaaatgcgcg cggataaagt tcgtgagatt gccgcaaaac tggggcgtca tgtggctatt
    29701 ctgggtgacc tccaggggcc caaaatccgt gtatccacct ttaaagaagg caaagttttc
    29761 ctcaatattg gggataaatt cctgctcgac gccaacctgg gtaaaggtga aggcgacaaa
    29821 gaaaaagtcg gtatcgacta caaaggcctg cctgctgacg tcgtgcctgg tgacatcctg
    29881 ctgctggacg atggtcgcgt ccagttaaaa gtaatggaag ttcagggcat gaaagtgttc
    29941 accgaagtca ccgtcggtgg tcccctctcc aacaataaag gtatcaacaa acttggcggc
    30001 ggtttgtcgg ctgaagcgct gaccgaaaaa gacaaagcag acattaagac tgcggcgttg
    30061 attggcgtag attacctggc tgtctccttc ccacgctgtg gcgaagatct gaactatgcc
    30121 cgtcggctgg cacgcgatgc aggatgtgat gcgaaaattg ttgccaaggt tgaacgtgcg
    30181 gaagccgttt gcagccagga tgcaatggat gacatcatcc tcgcctctga cgtggtaatg
    30241 gttgcacgtg gcgacctcgg tgtggaaatt ggcgacccgg aactggtcgg cattcagaaa
    30301 gcgttgatcc gtcgtgcgcg tcagctaaac cgagcggtaa tcacggcgac ccagatgatg
    30361 gagtcaatga ttactaaccc gatgccgacg cgtgcagaag tcatggacgt agcaaacgcc
    30421 gttctggatg gtactgacgc tgtgatgctg tctgcagaaa ctgccgcagg gcagtatccg
    30481 tcagaaactg ttgcagccat ggcgcgcgtt tgcctgggtg cggaaaaaat cccgagcatc
    30541 aacgtttcta aacaccgtct ggacgttcag ttcgacaatg tggaagaagc tattgccatg
    30601 tcagcaatgt acgcagctaa ccacctgaaa ggcgttacgg cgattattac catgaccgaa
    30661 tcgggtcgta ccgcgctgat gacctcccgt atcagctctg gtctaccaat tttcgccatg
    30721 tcgcgccatg aacgtacgct gaacctgact gctctctatc gtggcgttac gccggtgcac
    30781 tttgatagcg ctaatgacgg cgtagcagct gccagcgaag cggttaatct gctgcgcgat
    30841 aaaggttact tgatgtctgg tgacctggtg attgtcaccc agggcgacgt gatgagtacc
    30901 gtgggttcta ctaataccac gcgtatttta acggtagagt aagtacgttg ccggatgcgg
    30961 cgaaaacgcc acatccggcc tacagttcaa tgatagttca acagatttcg aatattctga
    31021 agcaaacttg aacttatcat caggcgaagg cctctcctcg cgagaggctt ttttatttga
    31081 tgggataaag atctttgcgc ttatacggct ggatttcgcc cggtttgcga gttttcagca
    31141 attttagtat ccaggtgtat tgttctggtc gcggaccaac aaaaatctcg acttcttcat
    31201 tcatccgccg cgcaatcgta tgatcatccg cctctaacag atcatccatc ggtgggcgca
    31261 cctgaatcgt cagacggtgc gtcttgccat cataaatcgg aaacagcgga acaacgcgcg
    31321 cacggcacac tttcatcaaa cgaccaatcg cgggcaacgt cgctttatag gtggcaaaga
    31381 aatcaacaaa ttcgctgtgc tcagggccat gatcctgatc gggtaaataa tatccccagt
    31441 agccctgacg taccgactgg atgaatggtt taataccatc atttctcgca tgcagacgac
    31501 cgccaaaacg acgacgcacc gtgttccaga cataatcaaa aaccgggttg ccctgattat
    31561 ggaacatcgc tgccattttc tgcccttgcg aggccatcag catggcagga atatcgacgg
    31621 cccaaccgtg cggcaccaga aagataactt tctcgttatt acgccgcatc tcttcgatga
    31681 tctccagccc ttgccagtca acgcgcggct gaattttctc cggcccgcgt attgccaact
    31741 cagccatcat tgccatcgct tgcggcgcgg tggcaaacat ctcatcaaca atcgcttcgc
    31801 gttcagcttc actacgttct ggaaagcaga gcgacagatt gattaacgca cgacggcgtg
    31861 agctttttcc cagtcgtccg gcaaaacgtc ccagccgtgc cagaatggga tcacggaact
    31921 ttggcggcgt taaagcgata cccgccatcg ctgctacgcc cagccatgct ccccagtagc
    31981 gcgggtggcg aaaggattta tcaaactcag gaatgtattc gctattattt tttttcgttt
    32041 ccatgctttt ccagtttcgg ataaggcaaa aatcaatctg gtgatagtgt agcggcgcaa
    32101 cttgccccgc accaaataaa aaaagccggt actgacttcg taccggctgc gaatggatgt
    32161 taattaatca aaccgtagct gcggcacaat ctctttggcc tgcgccagga attcgcgacg
    32221 atcggagccg gtcagccctt cggtacgcgg cagttttgcc gtcagcgggt ttacggcctg
    32281 ctggtttatc catacttcat agtgcaaatg cggcccggtt gaacgtccgg tattaccgga
    32341 aagcgcgata cggtcgccac gtttcacctt ctgtcccggt ttcaccagaa tcttgcgcaa
    32401 gtgcatataa cgcgtggtgt agctgcgacc atgacgaata gccacataat aacctgctgc
    32461 gccactgcgt ttggcaacca ccacttcacc gtcacccact gaaagcactg gcgtaccctg
    32521 cggcatggcg aaatcaacac ctctgtgtgg tgcaacgcga ccggtcaccg gattagtacg
    32581 acgcgggtta aagttagacg agatacggaa ctgtttcgcc gtcgggaatc gcaagaatcc
    32641 tttcgccaga ccagtaccgt tacgatcgta gaatttgcca tcttcagcgc ggatagcgta
    32701 ataatcttta ccttctgaac gcaaacgtac gcccagcagc tggctttgct cacgtttacc
    32761 atcaagcatt tctcgagaca ttaataccgc aaattcatcg ccttttttca gtttgcggaa
    32821 atccatttgc cactgcatgg ctttaatcac tgcgctcact tcggcgctgg ttaaaccggc
    32881 gtttctggcg ctggcaacaa agctcccccc gacggtacct ttcagcagat tgttaaccca
    32941 ctctccttgc tgcatttcgc tggtcatttt aaaaccgtta gcggcagtac ggtcataggt
    33001 tcgggtttca cgacgagaca cttcccaggt gaggcgctgc agttcgccgt ccgcggttaa
    33061 tgtccaggag agttgttgac cgattttcag gttacgcaat tctttgtcgg cagcagccag
    33121 ttgggtgata tcacccatat caataccata ctgattgaga atgctgctta gcgtatcgcc
    33181 agtggaaaca acatattcat gcacgcccgc ttcaccggcg attttgtcat ccagttcgtc
    33241 ctgaggaatg gcttcatctt cttgtgcagc ttgatcaatc ggctcactgg cttcaggtaa
    33301 gagcgaacga atttcgttct gttccagctc aatggttttg acaattggcg tggcatcgcg
    33361 gtgataaaca tagggccgcc agacagcgac ggccagagta agaacggtga gcgaccccaa
    33421 cataacgcgg tgtggtcgcg gtaaattatt aaacgccagg gcgacagagc gggctatctg
    33481 ttgcacgtaa tcacttcctc attaatctcc tttcaggcag ctcgcatact ggttggctaa
    33541 ttgactcagg aattctgaat agcttgtttt acccagtttg atattcgtcc ccaggggatc
    33601 caacgttccc atacgaacgg atgtccctcg tgcgacgctc tcaacgaccg ctggcctgaa
    33661 ctgtggctca gcaaaaacgc aggttgcttt ttgctcaacc aactgtgttc ttatttcatg
    33721 taaacgctgc gcgccaggtt gaatctcagg gttaacggta aaatgaccaa gcggtgtcag
    33781 tccaaactgt ttttcgaaat agccgtaagc atcgtgaaaa acgaaataac ctttcccctt
    33841 gagcggcgcg agctcgttac caacctgcgt ttcggttgag gctaattgtg cctcaaaatc
    33901 cttcaggttg gcgtcaagtt tggctcgact ttgcggcata agttccacta attttccatg
    33961 gattgcaacc gctgtagccc gcgctatctc tggggaaagc caaagatgca tgttgaaatc
    34021 gccgtgatgg tgatcttcgt cacttttttc cgcgtggtcg tgatcatcat catcgccgtg
    34081 aatacttttc atcagtagcg gtttcacatc ttcaagctgc gcaatcgtta cctgcttcgc
    34141 ttctggtaat ttgcttaccg gtttttgcat aaacgcttcc atctccgggc caacccaaac
    34201 gactaagtcc gcgttctgta agcgttttac atccgatgga cgcagtgaat aatcatgttc
    34261 tgaagcgccg tcaggcagta aaacctccgt ttctgttaca ccatcagcaa tggcagaagc
    34321 gatgaaccca acgggtttaa gcgaagcgac aacggcggca tctgcggcct gtgttgcacc
    34381 tccccagaga gcggcggata atgctgcgaa aagaagcgtt tttttatgta acataatgcg
    34441 accaatcatc gtaatgaata tgagaagtgt gatattataa catttcatga ctactgcaag
    34501 actaaaatta acatgacaag tctggtttcc ctggaaaatg tctcggtttc ttttggccaa
    34561 cgccgcgtcc tctctgatgt gtcgctggaa cttaaacctg gaaaaatttt gactttactt
    34621 gggccaaatg gcgcaggtaa gtcgacactg gtacgggtag tgctcgggct ggtaacaccc
    34681 gatgaagggg ttatcaagcg caacggaaaa ctgcgcatcg gctatgtacc gcagaagctg
    34741 tatctcgaca ccacgttgcc actgaccgta aaccgttttt tacgcttacg ccctggtaca
    34801 cataaagaag atattttgcc tgcactgaaa cgtgtccagg ccgggcacct gattaacgca
    34861 ccgatgcaaa agctctctgg tggcgaaacg cagcgtgtac tattagcgcg agcattgtta
    34921 aatcgcccgc aattattagt gctggatgaa cccactcaag gcgtggatgt aaatggccag
    34981 gtggcgttat atgaccttat tgaccaactg cgtcgtgaac tggattgtgg cgttttaatg
    35041 gtttctcacg atctgcatct ggtgatggca aaaaccgatg aagtgctgtg cctgaatcac
    35101 cacatttgtt gttccggcac accggaagtt gtttccctgc atccggagtt tatttcaatg
    35161 tttggtcctc gtggtgctga acaactgggt atctatcgcc atcatcataa ccatcgtcac
    35221 gatttacagg gacgaattgt tttgcgtcgg ggaaatgatc gctcatgatt gaattattat
    35281 ttcccggttg gttagccggg atcatgctcg cctgtgccgc gggtccgctg ggttcgtttg
    35341 tagtctggcg tcgtatgtct tatttcggtg atacgctggc tcatgcctca ttacttggcg
    35401 tcgcgtttgg tttgttgctg gacgtgaatc cattctatgc ggtaattgcc gttacgctgc
    35461 tcctggcggg cggtctggta tggctggaga agcgtccaca gctggcgatt gacacgttat
    35521 tagggattat ggcgcacagt gccctgtcgc tgggcctggt ggtcgttagt ctgatgtcta
    35581 acattcgtgt tgatttgatg gcttacctgt tcggtgattt gctggcagtg acgccagaag
    35641 atctcatcgc tattgcgatt ggcgtggtca tcgtggtggc tattttgttc tggcaatggc
    35701 gcaatttgct gtcgatgacg attagcccgg atctggcgtt tgttgatggt gtgaaattac
    35761 agcgcgtgaa attgttgttg atgctggtga cggcattgac gattggtgta gcgatgaaat
    35821 ttgttggcgc gttgattatt acttcactgc tgattattcc tgcagctact gcgcgtcgct
    35881 ttgcccgtac gccggaacag atggctggtg tcgctgtttt ggtggggatg gtggcagtaa
    35941 ctggcggttt aaccttttcc gcgttttacg atacgccggc gggtccgtcg gtggtgctat
    36001 gtgcggcact gttatttatt ctcagtatga tgaaaaagca ggccagctaa tctatcgctg
    36061 aacacatttg tcggatgcgg cgcgagcgcc ttatccgacc tacggttcgc tatctctggt
    36121 aggccggata agacgcgaat agcgtcgcat caggcacact gccaatgtcg gatgcggtgc
    36181 gagcgaccaa tccgacttac ggcatttctg gcggcgttat gccaaagtga ttccacgccc
    36241 gcgtcgtcgc catacgccca cgcggtgtac gctgcaaaaa gccttgctga atcagataag
    36301 gctccagcac atcctcaatg gtttcacgtt cttcgccaat agccgccgcc aggttatcca
    36361 gacctacagg cccaccaaag aatttatcga ttaccgccag caacaatttg cggtccatat
    36421 aatcaaaacc ttcagcatcg acattcagca tatccagcgc ctgagcagcg atatctgccg
    36481 agatggtgcc atcgtgcttc acttcggcga aatcacgcac tcgacgcagc agacggttgg
    36541 caatgcgcgg cgtaccgcga gcgcgacgag caacttccag cgcgccgtca tcactcatct
    36601 caagccccat aaagcgtgcg ctgcgactga cgatatattg cagatccggc acctgataaa
    36661 actccagacg ttgcacaata ccaaaacggt cgcgcaacgg tgatgtcagc gaacctgcgc
    36721 gtgtggttgc accaatcagg gtaaacggcg gcaaatcaat tttaatggag cgtgccgccg
    36781 gaccttcacc aatcatgata tccagctggt agtcttccat tgccggatac aacacctctt
    36841 caacaactgg cgatagacgg tggatctcat caataaacag cacgtcatgc ggttcaaggt
    36901 tagtgagcat cgctgccaga tcgcccgcct tttccagcac cggaccagaa gtcgtgcgta
    36961 aattaacgcc catttcattg gcgacaatgt tggcaagcgt agttttaccc aaccccggag
    37021 gaccaaaaat caacaaatga tcgagggcat cgccgcgcag tttcgctgct ttgatgaaaa
    37081 tctccatctg tgaacgaacc tgcggctgac caacatactc ttccagtaat ttagggcgaa
    37141 tggcgcgatc tgctacatct tccggcaaag tggtaccggc agaaatcaga cggtctgctt
    37201 caatcatcct ttacctcata acgcggcgcg tagggcttcg cgaattaatg tttcactgct
    37261 ggcgtcaggg cgagcgattt tgctcaccat gcggcttgct tcttgtggtt tatagcccag
    37321 cgccaccagc gcggcaaccg cttcttgttc agcatcgtcg gtcgccgggc tggcaggaga
    37381 cgtgagcacc agatcggcgg ctggcgtaaa gagatcgccg tgcaaacctt taaatcggtc
    37441 tttcatttcg acaattaaac gctcggcggt ttttttacca atgcccggca gtttcaccag
    37501 tgctcccact tcttcacgtt caacggcatt aacgaactgc tgcgctgaca ttccggagag
    37561 gatcgccagc gccaacttcg ggccgacgcc gttggttttg attaactctt taaacaacgt
    37621 gcgctcttgc ttattgttaa agccgtacag cagctgcgcg tcttcacgca ccacaaagtg
    37681 ggtgaaaacg atcgcttcct gacccgcttc agggagttca taaaaacagg tcatcggcat
    37741 atgcacttca tagcctacgc cgcccacttc aattaacacc agcgggggtt gtttttcaat
    37801 gatgatgcct ctgagtctgc ctatcacatg acgctcctgc gtaatgaatc aaagataatg
    37861 ctgtatgata aaaaaatgct ggatagatat ccagcgaagg atgaagaaaa cttgcgaggc
    37921 gtcccaacga tctgaaaaat ggcgcagtat aatttattct acaggttata ttggaagcaa
    37981 atatttgaat attacatatt cagtgaagaa atgcgtaata aaaatataca ttgcgactcc
    38041 tgaaataata aattttttat gctattacgt atattcatat ctatttcaat ggaatgacaa
    38101 cgtgaatatt aattaccctg ctgaatatga aattggatat atcgtcttta catgtatagg
    38161 tgctgcctta tttggtcaaa tatcagctgc atcaaattgc tggagtaatc acgtcgggat
    38221 cattatcggt cataacggtg aagacttcct ggttgcagaa agccgggttc ccctttcaac
    38281 catcaccacg ctatctcgtt ttattaaacg ttcgtctaat caacgctatg ctataaagcg
    38341 attagacgct gggctgacag aacaacaaaa acaacgaatt gttgaacagg ttccctcccg
    38401 gctacgcaaa ctttaccaca ccggttttaa atacgaatct tcgcgccagt tctgttcaaa
    38461 atttgttttt gatatttata aagaagcgct atgcattccg gtgggtgaaa tagaaacgtt
    38521 tggagaactg ttaaatagca atccgaatgc aaaactcact ttctggaaat tctggttctt
    38581 aggttctatt ccgtgggagc gtaaaaccgt cacgccagcc agtttgtggc atcatccggg
    38641 tttggtgttg attcacgcgg agggagttga aacgcctcag cctgaactga ccgaggcggt
    38701 ataacttaac gcagtcgccc tctcgccagg ttcagccgcg attcgctcat ctgcatcgca
    38761 ttctgactaa cgtggcagtg ggtgatggca atcgccagtg catcggcggc atccgcttgt
    38821 ggattagcgg gcagtttcag caaggtgcgg accatatgct gcacctggct tttctcggcg
    38881 ctaccaatgc ctaccaccgt ttgctttacc tgacgtgccg cgtattcaaa tactggcaac
    38941 tcctgattca ccgccgccac aatcgccacg ccgcgcgcct ggcccagttt cagggctgag
    39001 tcagcgttct tcgccataaa gacttgttca atggcgaaat aatcaggctg gaactgggtg
    39061 atgatttccg tcacgcccgc atagatgagc ttcagacgag acggtaaatc atccactttg
    39121 gtgcgtatgc atccgctacc caggtaggac agttgcctgc ctacctggcg aattacgcca
    39181 tagccggtca cgcgcgaacc cggatcaatg ccgagaataa tagccatcac gcgtctccgt
    39241 tttgctgttt agcaggcctc atcagagagt cgctgcgacc tcatcagaga tttcaccgtt
    39301 atggtaaact tcctgcacgt cgtcgcaatc ttccagcata tcgatcagac gcatcagttt
    39361 cggtgcggtt tctgcatcca tatcagcttt ggtggacggg atcatggaaa cttccgcgct
    39421 gtctgctttc agacctgccg cttccagagc gtcgcgcact ttacccattt cttcccatgc
    39481 agtgtagaca tcaatcgcgc cgtcatcata ggtcacaacg tcttcagcac ctgcttccag
    39541 agctgcttcc atgatggtgt cttcatcgcc tttctcgaag gagatcacgc cttttttgct
    39601 gaacagatag gctacggaac catctgtacc gaggttaccg ccacatttgc tgaatgcatg
    39661 acgcacttca gcaacggtac ggttgcggtt gtcagacaga cattcaatca tgattgccgt
    39721 gccgccagga ccgtaacctt cgtagatgat ggtttccatg tttgcatcat catcaccgcc
    39781 cacaccacgt gcaattgcgc ggttcagtgt gtcacgggtc atgttgttag acagtgcttt
    39841 atccaccgcc gcacgcagac gcgggttagc gtccggatcg ccaccgccca gcttagccgc
    39901 ggttaccagc tcacgaatga ttttagtgaa gattttaccg cgcttagcat cctgcgcagc
    39961 tttacgatgt ctggtgttgg cccatttact atgacctgcc ataaaaatat ctccagatag
    40021 ccctgcctgt tcaggcagcg ttaattacaa actgttcaat cgcctgccgg ttgctccagg
    40081 acttagtgag cgccgccgca gcaggcgcat caagccactt gtaagccaga tgttcagtga
    40141 aaacgatctg ccgctcgtgc ggaagcgcaa gacagaacca tgattccgta ttacgcgtca
    40201 cgcccggcgc atagcgatga cgtaaatgtg aaaaaatttc aaactctacc gtgcgctgac
    40261 agtcaattaa ggtcagttgt tcagcgacaa catcaatggt gacctcttcc tttacttcgc
    40321 gcatggcagc ttgcggcgcg gtttcaccct cttccacgct gccggttacc gactgccaga
    40381 aatcgggatc gtcacgccgc tgcaacatca gcacccgttt cgtatcttgt gcatagatga
    40441 ccactaagat cgaaacggga cgcttataca ctttatcctc cacgctgcct cttcgttgac
    40501 tgccttcgct catcccattc acatagttat ctatgctcat gggagttcac tcagttgccg
    40561 ccttgatgca acgcgaatga tttcgtgtat ttgagtcata tcagttattc tcagccttct
    40621 tcacaacctg aatgctcagc tcagccagtg cagtcgggtt agcaaagctc ggtgcttcag
    40681 tcatcagaca cgctgccgcc gtggttttcg ggaaggcgat aacgtcacgg atattgtcgg
    40741 tgccggtcag cagcatggtc agacggtcaa gaccgaatgc cagacctgcg tgcggcggag
    40801 taccgtattt cagagcgtcg agcaggaagc cgaatttctc gcgctgttcc tcttcgttga
    40861 tacccagaat accaaacacc gtctgctgca tatcaccatt atggatacgt actgaaccac
    40921 cgcccacttc gtaaccattg atgaccatat cgtaagcgtt cgccaccgca ttttccggtg
    40981 cagctttcag ttctgcagca gtcatgtctt tcggtgaggt gaacggatgg tgcattgccg
    41041 tcaggccgcc ttcaccgtcg tcttcaaaca tcgggaagtc gataacccac agcggtgccc
    41101 atttgctttc gtcggtcaga ccaaggtctt tacccacttt caggcgcagt gcgcccatcg
    41161 cgtcggcaac aattttcttg ttgtcggcac cgaagaaaat catatcgcca tcttgcgcgg
    41221 cagtacgatc caggatggct tcgatgattt ctgcattaag gaacttcgct accgggctgt
    41281 tgataccttc cagacctttc gcgcgttcgt taactttgat gtaagccaga cctttcgcgc
    41341 cgtagatttt aacgaagtta ccgtattcgt cgatctgctt acgggtcagc gatgcgccgc
    41401 ccggaacgcg cagagcggca acgcgacctt tcggatcgtt cgccggacct gcaaatactg
    41461 caaactcaac agatttcagc agatcagcaa cgtcggtcag ttccatcggg ttacgcagat
    41521 ccggtttatc agaaccataa cggcgttctg cttccgcaaa ggtcattacc gggaaatcgc
    41581 ccagatccac gcccttcact tccagccaca gatggcgcac cagcgcttcc atcacttcac
    41641 gcacttgcgg cgcggtcatg aaagaagttt ccacatcgat ctgagtaaat tcaggctgac
    41701 gatcagcgcg caggtcttcg tcacggaagc atttaacgat ctgatagtag cggtcaaagc
    41761 cggacatcat cagcagctgt ttgaacaact gcggggattg tggcagcgcg tagaatttac
    41821 ctttgtgcac acgagaaggc accaggtagt cacgcgcgcc ttccggcgtg gctttggtca
    41881 gcatcggagt ttcgatgtcg aggaagccgt ggtcatccat aaaacggcgc accaggctgg
    41941 tgattttagc gcgggttttc aggcgctgag ccatttccgg gcgacgcagg tcgaggtagc
    42001 ggtatttcag acgcgcttct tcggtgttga cgtggttaga gtcaagcggc agaacatctg
    42061 cgcggttgat gatagtcagc gaggacgcca gcacttcgat ttcgccggtc gccatatcgc
    42121 ggttaatatt tttttcgtca cgcgcacgta cggtgcccgt gacctgaatg cagaactcat
    42181 tacgcagttc agaggccagc tttaacgcgt ccgcacgatc cggatcgaaa aatacctgca
    42241 cgataccttc gcggtcgcgc atatcgatga agatcaggct accaagatca cgacgacggt
    42301 tgacccaacc acacagagtc acctgctgcc ccacgtggga caaacggagc tgtccacaat
    42361 attctgtacg catgagatat cccttaactt agctgccggc ggatgccccc tgctgcgcag
    42421 gtgaccaagt cgcagcgtta gctgtatgtc acaactgaat gaaaaaaggc ggctattata
    42481 ctggaaattc tgccgcaccg taagagcctg gcccgcgctg gaacgcctcg ttaccacttt
    42541 atatcgggcc tgaaatcaga ctctacgcca gtttgctata aaggtgttgc ccgaactcat
    42601 aaaaattaac aaaatttgtc gttccgccat cggctaatcg cattaaggtg tgaggcacga
    42661 ttttgttttt tcaggagtca ttatgcttga acttaatgct aaaaccactg cgctggtggt
    42721 gatcgattta caagaaggca tcttaccttt tgccggtggt ccacatactg ccgatgaggt
    42781 agttaatcgc gccgggaagc tggcggcgaa atttcgcgcc agcggtcagc ccgtgtttct
    42841 ggtgcgcgtt ggctggtctg ccgattacgc cgaagcatta aaacagccgg ttgatgcccc
    42901 ctcccccgct aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac
    42961 tgacagtgat atcgaaatca tcaaacgtca gtggggtgcg ttttacggta cggatctgga
    43021 gttgcaactg cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat
    43081 cggtgttgaa tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga
    43141 agacgcctgt agtgccgcca gcgccgagca gcacaataac agcatcaacc atatctaccc
    43201 acgcatcgcc cgtgtgcgca gtgtggaaga gatccttcac gcgttatgat ttacatcggt
    43261 ctaccgcaat ggtcgcatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat
    43321 gcccgccact ttaactgcgt ggagggcaac accacgcttt acgccctgcc gaaacccgag
    43381 gttgtcctgc gctggcgtga gcagaccaca gatgacttcc gcttctgttt taagtttccg
    43441 gcgaccattt tgcatcaggc agcattacgg cattgcgatg atttagtgac tgaatttttg
    43501 acccgcatgt caccgttggc tccgcgcatt gggcaatact ggctgcaact gcctgccaca
    43561 ttcggcccac gggagctgcc tgcgctttgg cattttctcg attctctccc tggcgaattt
    43621 aattatggtg tggaagtccg ccatccacag tttttcgcca aaggggaaga ggaacaaacg
    43681 cttaatcgcg gtttacatca gcgcggcgtt aatcgggtga ttttagacag ccgcccggtt
    43741 catgcagcat gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
    43801 ccggtacatg ctgtactgac ggcgacaaat ccgctgattc gttttatcgg tagtgatgat
    43861 atgacgcaaa accgggaatt atttcaggtc tggttacaga aattagcgca gtggcatcag
    43921 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
    43981 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
    44041 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc aatcggccat
    44101 tttaaaggga gtttgtatgg taagcgcgct gtatgccgtt ttaagtgcgt tgttattaat
    44161 gaagttctct tttgatgtcg ttcgcttgcg tttgcagtac cgcgttgcct atggcgacgg
    44221 cggttttagc gaactgcaaa gcgctattcg cattcatggt aacgcggtgg aatatattcc
    44281 catcgcgatt gtgttgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
    44341 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
    44401 tctgttccgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
    44461 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
    44521 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
    44581 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtagctgaa gtcttcccgg
    44641 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
    44701 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
    44761 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
    44821 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
    44881 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
    44941 tgctgaattt taccctgcaa ttcctggatc cttccgagcg ccaggcatta ctggataaaa
    45001 tttatcaagg actgaaccca ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
    45061 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
    45121 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
    45181 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
    45241 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
    45301 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
    45361 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
    45421 caacgcggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
    45481 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
    45541 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
    45601 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
    45661 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
    45721 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
    45781 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
    45841 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
    45901 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
    45961 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
    46021 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
    46081 gaagtgtggt tttgtagata tccgcgttgt ggatgtgtgc gttaccacta ctgaagagca
    46141 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
    46201 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
    46261 gtaaaggtct ggcattactg ccggatgcgg cttgaatgcc ttatccggcc tacaaaccct
    46321 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
    46381 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt atgcctgaat
    46441 agctggctga tcaaacgccg ttaacttcgg cgcattgccc gtatattttt cgatatacac
    46501 cagcgcagag ttaccggcac aggcatttgc cagctgcgag ctggggatat ccgccgttaa
    46561 cacgttcgca ctgccgtttt tacacaagcc attttccaga tctggccatg caccttcatg
    46621 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca gaatctgacc
    46681 acgtttgttc cacacacgca ccagatcacc attcgcaata ccaaagcgag cagcatcttc
    46741 ggtgtgaata gtgattggtt cacgatctgc aaccgcatat tttttacgca gttccgcata
    46801 gttaagctga ctatgtaaac ggtgtgccgg atatgcggtc agaagctgca actgcttctc
    46861 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
    46921 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
    46981 cgcatttttc accggatcgg cgcggaaatc agcataacga acgtactgtt cgttcttctc
    47041 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca tcggcatagt
    47101 cacgcgttgc gcacgagcac ctttctgagc ggcatcatag aaaaatttca gccacgccat
    47161 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttcagtaatt cagcaagatc
    47221 ggcaaatacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
    47281 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
    47341 ggtgatcggt aatacgatat ctgcatgttt agcagctgcg gtccagtagc attcagaaac
    47401 gacgatcatc tccggtttct gccatgcttt gatcagacga ttagtatcct ggtgatgggt
    47461 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
    47521 gttatgctga tatttgccac caggattttc cagcgcatcg acgatacggg caacaggaat
    47581 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
    47641 ttccggcagc acgccgccaa cacgcgtcgg attgccaccg ttggagtaat gataagagaa
    47701 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
    47761 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagccagcca tcagcattgt
    47821 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
    47881 ttccgccgcc caggccgcgc tcttcggagt attatcgctc ttacctgtca gatactcttc
    47941 aaactgcgga tacccggtgg tgtatttttc aaggaacact ttatcgtgtt taccttgtgt
    48001 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
    48061 ccaggtagca ttatcaccaa agaactcgat ggtttcggag cggataggat caatggcaat
    48121 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
    48181 gctccaggca atttttaagg tattcagcgg gttcatcccc cacagcacca cagcctggct
    48241 gttttccaga atcagcggcc aggaggtctg ctgttcatac acctctacag aaccgaccac
    48301 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
    48361 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
    48421 gcgccagccg tatgatccgg caaaaatagc agatggtccg ttagctttgc gaatgcgatc
    48481 gtgttgctca tgaatcagtt ttaatgcttg ctcccagctc acctgtacat aagtatcttc
    48541 gccacgacct ttcgccggtt gcagtggatt atcgagatag ctttttctta ccatcggatg
    48601 ctgaatacgt gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
    48661 ggttttcgcc agcgcgcctg ttgaagaaac aatcttgccg tccttcactt ctacgttcat
    48721 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
    48781 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
    48841 acgtcttgtt aatgtcataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
    48901 atctttggcg ttgtactgga aataccgcgt taaaatgtcc agttcgtttt cgctcatgct
    48961 ggttcgcgca cccattcctt tggcaatgga cggccatgca ttgacggtgt aatggtcagc
    49021 ggcaataggc gcatggcaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
    49081 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
    49141 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
    49201 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
    49261 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
    49321 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
    49381 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
    49441 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
    49501 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
    49561 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
    49621 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
    49681 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
    49741 taaataatcc atccctgact ttggaatatg gcaatctgca cattccgcac gtatcccttt
    49801 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
    49861 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
    49921 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
    49981 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
    50041 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
    50101 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatata
//
LOCUS       DAZGAN020000024        49925 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGAN020000024 DAZGAN020000000
VERSION     DAZGAN020000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 49925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.024"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..686
                     /locus_tag="ACHKYO_RS07945"
                     /old_locus_tag="pgaptmp_001589"
     CDS             <1..686
                     /locus_tag="ACHKYO_RS07945"
                     /old_locus_tag="pgaptmp_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HGU4428453.1"
                     /translation="ITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDDAVRS
                     TLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVNQIGGK
                     ERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQLTLLF
                     GMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMAKQI
                     ARLAEQSTD"
     gene            785..1798
                     /gene="ybjS"
                     /locus_tag="ACHKYO_RS07950"
                     /old_locus_tag="pgaptmp_001590"
     CDS             785..1798
                     /gene="ybjS"
                     /locus_tag="ACHKYO_RS07950"
                     /old_locus_tag="pgaptmp_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU4428454.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1795..2625)
                     /gene="amiD"
                     /locus_tag="ACHKYO_RS07955"
                     /old_locus_tag="pgaptmp_001591"
     CDS             complement(1795..2625)
                     /gene="amiD"
                     /locus_tag="ACHKYO_RS07955"
                     /old_locus_tag="pgaptmp_001591"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HGU4428455.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2622..2945)
                     /gene="ybjQ"
                     /locus_tag="ACHKYO_RS07960"
                     /old_locus_tag="pgaptmp_001592"
     CDS             complement(2622..2945)
                     /gene="ybjQ"
                     /locus_tag="ACHKYO_RS07960"
                     /old_locus_tag="pgaptmp_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HGU4428456.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            3071..3586
                     /gene="ybjP"
                     /locus_tag="ACHKYO_RS07965"
                     /old_locus_tag="pgaptmp_001593"
     CDS             3071..3586
                     /gene="ybjP"
                     /locus_tag="ACHKYO_RS07965"
                     /old_locus_tag="pgaptmp_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4428457.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3804..4532
                     /gene="artP"
                     /locus_tag="ACHKYO_RS07970"
                     /old_locus_tag="pgaptmp_001594"
     CDS             3804..4532
                     /gene="artP"
                     /locus_tag="ACHKYO_RS07970"
                     /old_locus_tag="pgaptmp_001594"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HGU4428458.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            4550..5281
                     /gene="artI"
                     /locus_tag="ACHKYO_RS07975"
                     /old_locus_tag="pgaptmp_001595"
     CDS             4550..5281
                     /gene="artI"
                     /locus_tag="ACHKYO_RS07975"
                     /old_locus_tag="pgaptmp_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HGU4428459.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            5288..6004
                     /gene="artQ"
                     /locus_tag="ACHKYO_RS07980"
                     /old_locus_tag="pgaptmp_001596"
     CDS             5288..6004
                     /gene="artQ"
                     /locus_tag="ACHKYO_RS07980"
                     /old_locus_tag="pgaptmp_001596"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HGU4428460.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            6004..6672
                     /gene="artM"
                     /locus_tag="ACHKYO_RS07985"
                     /old_locus_tag="pgaptmp_001597"
     CDS             6004..6672
                     /gene="artM"
                     /locus_tag="ACHKYO_RS07985"
                     /old_locus_tag="pgaptmp_001597"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HGU4428461.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            6964..7695
                     /gene="artJ"
                     /locus_tag="ACHKYO_RS07990"
                     /old_locus_tag="pgaptmp_001598"
     CDS             6964..7695
                     /gene="artJ"
                     /locus_tag="ACHKYO_RS07990"
                     /old_locus_tag="pgaptmp_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HGU4428462.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(7870..8997)
                     /gene="rlmC"
                     /locus_tag="ACHKYO_RS07995"
                     /old_locus_tag="pgaptmp_001599"
     CDS             complement(7870..8997)
                     /gene="rlmC"
                     /locus_tag="ACHKYO_RS07995"
                     /old_locus_tag="pgaptmp_001599"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HGU4428463.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVEHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(9038..9526)
                     /gene="ybjO"
                     /locus_tag="ACHKYO_RS08000"
                     /old_locus_tag="pgaptmp_001600"
     CDS             complement(9038..9526)
                     /gene="ybjO"
                     /locus_tag="ACHKYO_RS08000"
                     /old_locus_tag="pgaptmp_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HGU4428464.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(9586..10431)
                     /gene="potI"
                     /locus_tag="ACHKYO_RS08005"
                     /old_locus_tag="pgaptmp_001601"
     CDS             complement(9586..10431)
                     /gene="potI"
                     /locus_tag="ACHKYO_RS08005"
                     /old_locus_tag="pgaptmp_001601"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HGU4428465.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(10428..11381)
                     /gene="potH"
                     /locus_tag="ACHKYO_RS08010"
                     /old_locus_tag="pgaptmp_001602"
     CDS             complement(10428..11381)
                     /gene="potH"
                     /locus_tag="ACHKYO_RS08010"
                     /old_locus_tag="pgaptmp_001602"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HGU4428466.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(11391..12524)
                     /gene="potG"
                     /locus_tag="ACHKYO_RS08015"
                     /old_locus_tag="pgaptmp_001603"
     CDS             complement(11391..12524)
                     /gene="potG"
                     /locus_tag="ACHKYO_RS08015"
                     /old_locus_tag="pgaptmp_001603"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HGU4428467.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(12619..13731)
                     /gene="potF"
                     /locus_tag="ACHKYO_RS08020"
                     /old_locus_tag="pgaptmp_001604"
     CDS             complement(12619..13731)
                     /gene="potF"
                     /locus_tag="ACHKYO_RS08020"
                     /old_locus_tag="pgaptmp_001604"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HGU4428468.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     NLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(14082..14558)
                     /gene="ybjN"
                     /locus_tag="ACHKYO_RS08025"
                     /old_locus_tag="pgaptmp_001605"
     CDS             complement(14082..14558)
                     /gene="ybjN"
                     /locus_tag="ACHKYO_RS08025"
                     /old_locus_tag="pgaptmp_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HGU4428469.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(14646..15548)
                     /gene="rimK"
                     /locus_tag="ACHKYO_RS08030"
                     /old_locus_tag="pgaptmp_001606"
     CDS             complement(14646..15548)
                     /gene="rimK"
                     /locus_tag="ACHKYO_RS08030"
                     /old_locus_tag="pgaptmp_001606"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HGU4428470.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(15609..16331)
                     /gene="nfsA"
                     /locus_tag="ACHKYO_RS08035"
                     /old_locus_tag="pgaptmp_001607"
     CDS             complement(15609..16331)
                     /gene="nfsA"
                     /locus_tag="ACHKYO_RS08035"
                     /old_locus_tag="pgaptmp_001607"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HGU4428471.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISDAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(16315..16602)
                     /gene="ybjC"
                     /locus_tag="ACHKYO_RS08040"
                     /old_locus_tag="pgaptmp_001608"
     CDS             complement(16315..16602)
                     /gene="ybjC"
                     /locus_tag="ACHKYO_RS08040"
                     /old_locus_tag="pgaptmp_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HGU4428472.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            16762..17019
                     /gene="grxA"
                     /locus_tag="ACHKYO_RS08045"
                     /old_locus_tag="pgaptmp_001609"
     CDS             16762..17019
                     /gene="grxA"
                     /locus_tag="ACHKYO_RS08045"
                     /old_locus_tag="pgaptmp_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HGU4428473.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(17049..17426)
                     /gene="ybjM"
                     /locus_tag="ACHKYO_RS08050"
                     /old_locus_tag="pgaptmp_001610"
     CDS             complement(17049..17426)
                     /gene="ybjM"
                     /locus_tag="ACHKYO_RS08050"
                     /old_locus_tag="pgaptmp_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HGU4428474.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            17696..19381
                     /gene="ybjL"
                     /locus_tag="ACHKYO_RS08055"
                     /old_locus_tag="pgaptmp_001611"
     CDS             17696..19381
                     /gene="ybjL"
                     /locus_tag="ACHKYO_RS08055"
                     /old_locus_tag="pgaptmp_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HGU4428475.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(19553..20092)
                     /gene="rcdA"
                     /locus_tag="ACHKYO_RS08060"
                     /old_locus_tag="pgaptmp_001612"
     CDS             complement(19553..20092)
                     /gene="rcdA"
                     /locus_tag="ACHKYO_RS08060"
                     /old_locus_tag="pgaptmp_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HGU4428476.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAGL"
     gene            20176..21384
                     /gene="ybjJ"
                     /locus_tag="ACHKYO_RS08065"
                     /old_locus_tag="pgaptmp_001613"
     CDS             20176..21384
                     /gene="ybjJ"
                     /locus_tag="ACHKYO_RS08065"
                     /old_locus_tag="pgaptmp_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4428477.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFIDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            21384..22199
                     /gene="ybjI"
                     /locus_tag="ACHKYO_RS08070"
                     /old_locus_tag="pgaptmp_001614"
     CDS             21384..22199
                     /gene="ybjI"
                     /locus_tag="ACHKYO_RS08070"
                     /old_locus_tag="pgaptmp_001614"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HGU4428478.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            22285..22569
                     /gene="ybjH"
                     /locus_tag="ACHKYO_RS08075"
                     /old_locus_tag="pgaptmp_001615"
     CDS             22285..22569
                     /gene="ybjH"
                     /locus_tag="ACHKYO_RS08075"
                     /old_locus_tag="pgaptmp_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HGU4428479.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(22610..23842)
                     /gene="mdfA"
                     /locus_tag="ACHKYO_RS08080"
                     /old_locus_tag="pgaptmp_001616"
     CDS             complement(22610..23842)
                     /gene="mdfA"
                     /locus_tag="ACHKYO_RS08080"
                     /old_locus_tag="pgaptmp_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HGU4428480.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            24127..24723
                     /gene="ybjG"
                     /locus_tag="ACHKYO_RS08085"
                     /old_locus_tag="pgaptmp_001617"
     CDS             24127..24723
                     /gene="ybjG"
                     /locus_tag="ACHKYO_RS08085"
                     /old_locus_tag="pgaptmp_001617"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU4428481.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGFTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            24781..25539
                     /gene="deoR"
                     /locus_tag="ACHKYO_RS08090"
                     /old_locus_tag="pgaptmp_001618"
     CDS             24781..25539
                     /gene="deoR"
                     /locus_tag="ACHKYO_RS08090"
                     /old_locus_tag="pgaptmp_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HGU4428482.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(25586..26788)
                     /gene="dacC"
                     /locus_tag="ACHKYO_RS08095"
                     /old_locus_tag="pgaptmp_001619"
     CDS             complement(25586..26788)
                     /gene="dacC"
                     /locus_tag="ACHKYO_RS08095"
                     /old_locus_tag="pgaptmp_001619"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU4428483.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            27035..27661
                     /gene="gstB"
                     /locus_tag="ACHKYO_RS08100"
                     /old_locus_tag="pgaptmp_001620"
     CDS             27035..27661
                     /gene="gstB"
                     /locus_tag="ACHKYO_RS08100"
                     /old_locus_tag="pgaptmp_001620"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HGU4428484.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(27658..28773)
                     /gene="yliI"
                     /locus_tag="ACHKYO_RS08105"
                     /old_locus_tag="pgaptmp_001621"
     CDS             complement(27658..28773)
                     /gene="yliI"
                     /locus_tag="ACHKYO_RS08105"
                     /old_locus_tag="pgaptmp_001621"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HGU4428485.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FVALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(28884..29267)
                     /gene="bssR"
                     /locus_tag="ACHKYO_RS08110"
                     /old_locus_tag="pgaptmp_001622"
     CDS             complement(28884..29267)
                     /gene="bssR"
                     /locus_tag="ACHKYO_RS08110"
                     /old_locus_tag="pgaptmp_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HGU4428486.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            29480..30805
                     /gene="rimO"
                     /locus_tag="ACHKYO_RS08115"
                     /old_locus_tag="pgaptmp_001623"
     CDS             29480..30805
                     /gene="rimO"
                     /locus_tag="ACHKYO_RS08115"
                     /old_locus_tag="pgaptmp_001623"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HGU4428487.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(30852..32180)
                     /gene="dgcI"
                     /locus_tag="ACHKYO_RS08120"
                     /old_locus_tag="pgaptmp_001624"
     CDS             complement(30852..32180)
                     /gene="dgcI"
                     /locus_tag="ACHKYO_RS08120"
                     /old_locus_tag="pgaptmp_001624"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU4428488.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDKFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(32188..34536)
                     /gene="pdeI"
                     /locus_tag="ACHKYO_RS08125"
                     /old_locus_tag="pgaptmp_001625"
     CDS             complement(32188..34536)
                     /gene="pdeI"
                     /locus_tag="ACHKYO_RS08125"
                     /old_locus_tag="pgaptmp_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4428489.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(34714..35625)
                     /gene="gsiD"
                     /locus_tag="ACHKYO_RS08130"
                     /old_locus_tag="pgaptmp_001626"
     CDS             complement(34714..35625)
                     /gene="gsiD"
                     /locus_tag="ACHKYO_RS08130"
                     /old_locus_tag="pgaptmp_001626"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HGU4428490.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(35628..36548)
                     /gene="gsiC"
                     /locus_tag="ACHKYO_RS08135"
                     /old_locus_tag="pgaptmp_001627"
     CDS             complement(35628..36548)
                     /gene="gsiC"
                     /locus_tag="ACHKYO_RS08135"
                     /old_locus_tag="pgaptmp_001627"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HGU4428491.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(36566..38104)
                     /gene="gsiB"
                     /locus_tag="ACHKYO_RS08140"
                     /old_locus_tag="pgaptmp_001628"
     CDS             complement(36566..38104)
                     /gene="gsiB"
                     /locus_tag="ACHKYO_RS08140"
                     /old_locus_tag="pgaptmp_001628"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HGU4428492.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEKATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(38124..39995)
                     /gene="gsiA"
                     /locus_tag="ACHKYO_RS08145"
                     /old_locus_tag="pgaptmp_001629"
     CDS             complement(38124..39995)
                     /gene="gsiA"
                     /locus_tag="ACHKYO_RS08145"
                     /old_locus_tag="pgaptmp_001629"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HGU4428493.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(39982..40947)
                     /gene="iaaA"
                     /locus_tag="ACHKYO_RS08150"
                     /old_locus_tag="pgaptmp_001630"
     CDS             complement(39982..40947)
                     /gene="iaaA"
                     /locus_tag="ACHKYO_RS08150"
                     /old_locus_tag="pgaptmp_001630"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU4428494.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            41151..42386
                     /gene="moeA"
                     /locus_tag="ACHKYO_RS08155"
                     /old_locus_tag="pgaptmp_001631"
     CDS             41151..42386
                     /gene="moeA"
                     /locus_tag="ACHKYO_RS08155"
                     /old_locus_tag="pgaptmp_001631"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HGU4428495.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLATLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            42386..43135
                     /gene="moeB"
                     /locus_tag="ACHKYO_RS08160"
                     /old_locus_tag="pgaptmp_001632"
     CDS             42386..43135
                     /gene="moeB"
                     /locus_tag="ACHKYO_RS08160"
                     /old_locus_tag="pgaptmp_001632"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HGU4428496.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARYALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(43314..43976)
                     /gene="fsa"
                     /locus_tag="ACHKYO_RS08165"
                     /old_locus_tag="pgaptmp_001633"
     CDS             complement(43314..43976)
                     /gene="fsa"
                     /locus_tag="ACHKYO_RS08165"
                     /old_locus_tag="pgaptmp_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU4428497.1"
                     /translation="MELYLDTSYVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            44107..45006
                     /gene="ybiY"
                     /locus_tag="ACHKYO_RS08170"
                     /old_locus_tag="pgaptmp_001634"
     CDS             44107..45006
                     /gene="ybiY"
                     /locus_tag="ACHKYO_RS08170"
                     /old_locus_tag="pgaptmp_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGU4428498.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            45012..47444
                     /gene="ybiW"
                     /locus_tag="ACHKYO_RS08175"
                     /old_locus_tag="pgaptmp_001635"
     CDS             45012..47444
                     /gene="ybiW"
                     /locus_tag="ACHKYO_RS08175"
                     /old_locus_tag="pgaptmp_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HGU4428499.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALTHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            47590..48405
                     /gene="ybiV"
                     /locus_tag="ACHKYO_RS08180"
                     /old_locus_tag="pgaptmp_001636"
     CDS             47590..48405
                     /gene="ybiV"
                     /locus_tag="ACHKYO_RS08180"
                     /old_locus_tag="pgaptmp_001636"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HGU4428500.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            48557..49822
                     /gene="ybiU"
                     /locus_tag="ACHKYO_RS08185"
                     /old_locus_tag="pgaptmp_001637"
     CDS             48557..49822
                     /gene="ybiU"
                     /locus_tag="ACHKYO_RS08185"
                     /old_locus_tag="pgaptmp_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HGU4428501.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGHFTLADLNIHGKRALGMDV"
BASE COUNT        12385 a        13193 c        12334 g        12013 t
ORIGIN      
        1 tgattacttc cgtaccgccc accaccgcca gggcgttgat tcaggggctg aaacacgatc
       61 tgctggcgga tgataccgcg ctacgtgcac tcatcccaca acggctgatc gctttcgatg
      121 acgcggtacg tagcacgttg aaagaggagg aaaaactggt caactccagc gactggggct
      181 acgacgctca ggcctttgcc cgctggcgac cggagtacgg ttattttgcc aaacaggcgg
      241 ggtttaccgt taaaacgtcc gccagccttg ctgctttatg gcaggtagtg aaccaaatcg
      301 gcggtaaaga gcgttatttc tttggcaata ttttgtggca gacacgggcg ttgatggacc
      361 gcgcgatcgg tcataagctg gcgaaaggcc gcccggagcg cgaatattta cagactggcg
      421 atgcggtaga tagctggaaa gtgattgtcg ttgaaccgga aaaacaactt acgttgttat
      481 ttggcatgaa agcgccgggg ctgggacgac tgtgttttag cctggaagat aaaggcgact
      541 atcgtactat cgatgtccgc gctttctggc atccgcacgg tatgccgggg ctgttttact
      601 ggttattgat gatccccgcg catctgttta tttttcgcgg aatggcaaaa caaatcgcca
      661 gactggcaga acaaagcaca gattaatcga caaaacctgc gttattcttt catgtttccc
      721 attgcatcat gggggaaatc acggaagaat gcgcacgtta ttttctcaac aacagtggat
      781 tgatatgaag gtactggtta ccggcgccac cagcggctta ggtcgaaacg cggtagagtt
      841 tttatgccag aaaggcatca gcgtgcgagc gaccggtcgc aacgaggcaa tgggcaaatt
      901 gctggaaaaa atgggcgcag agtttgttcc ggcggatctg accgagctgg tttcatcaca
      961 agctaaagtg atgctcgcgg gcattgatac gctgtggcac tgctccagct ttacctcacc
     1021 ctgggggaca caacaggctt tcgatctggc taacgttcgc gccactcgcc gcctgggtga
     1081 atgggctgtc gcctggggtg tgcgtaactt tattcatatc tcttccccct ccctgtactt
     1141 cgattatcac caccatcgcg atattaaaga agattttcgc cctcaccgct tcgccaacga
     1201 gtttgcccgc agcaaagcag ccagcgaaga agtgatcaat atgctttcgc aggcgaatcc
     1261 acaaacgcgc tttactattc tgcgcccaca aagtctgttc ggaccgcacg ataaagtctt
     1321 tattccccgt ctggcgcata tgatgcacca ctacggcagt attctgttac cgcatggcgg
     1381 cagtgcgctg gtcgatatga cctactatga aaatgccgtg cacgcaatgt ggctggcaag
     1441 ccaggaagcc tgcgataagc taccttccgg gcgtgtgtac aacatcacca acggcgagca
     1501 tcgcacactg cgcagcatcg tgcagaagct gatcgacgag ttgaatattg actgtcgtat
     1561 tcgttccgtc ccctacccga tgctggatat gatcgcccgc agcatggagc gtttaggccg
     1621 caagtcagca aaagagccgc cgctgaccca ctacggcgtc tccaagttga attttgactt
     1681 tacgctggat attacgcgtg cgcaggaaga gttaggttat cagccggtca tcaccctgga
     1741 tgaaggtatc gagaaaactg ccgcctggct acgcgaccac ggaaaactgc cgcgctaatc
     1801 ctgcccgtat ttctccagca atgcttcggc aatcgcctga gtttctgcat ccgcttcgcc
     1861 gttatataac gtcgggcgga aatgcatctg gaatgccata atcacgcgcc gctgctcgcg
     1921 cggtgtcata tcaggtttaa cgtcataacc gtagcgcgcc aaaagctcca gcaatgacgc
     1981 ggtatctacc ggtgtgtgcg gcgcgcgccc ggcaaggtaa aagttaaccc gctgcgcatc
     2041 cggccaggca ccaatcccct gctgcgccag ttgctgccag ggaaataatg gccccggatc
     2101 gtctttgcgc tgcggtgcga tatccgcatg tgccactacg ttttccggct tgatgtgata
     2161 acgggcaata atatctttcg ccagtggaat aagcgcctga atctgtgccg gttcaaacgg
     2221 ggcaaaatat ttcactccgg ccgatttttg ccagccacgg ttttccagct caatgccaat
     2281 agaggtgtcg ttaaggcgcg ttgccccgcg ccaggcgcta atccccgcat gccaggccag
     2341 ttcttgttcc ggcaccagtt gccagatgcg cggtttaccg ttgtatcgcg gtggtaccgc
     2401 agggaccaga taatgcgagc tgacctgctt atcggtcagt gtcgccagcg agctatcaaa
     2461 atcatctgcg gtgtagtgga tcaccagcac tttaatgcgc ggatacgccg cctgcgcctg
     2521 gcgtcgggta tcaagctgat atccctcttt ctcgacaatg cctttttcgc ctgcacaccc
     2581 tgccagtaac agagcggcag cgatcagcca gaagactctt ctcatcgacg cgttttcacc
     2641 gccgtaccgc tgacgctaac catcagcata ctgccgtttt gcccgaccgt ttcgtagtcg
     2701 atatcaatac cgacgacggc atcggccccc agcgcccggg cctgggagcc aagttcctca
     2761 aaggcgatct cccgcgcttt gcgcagttct ttttcatacg caccggagcg tccgccaacg
     2821 atatcgcgga taccagcaaa gaaatcacgg aaaatattgg cacctaaaat cgcttcgccg
     2881 gtcaccacac cgcaatattc aacgatggtc tggccttcca gagttggggt tgtagaaaat
     2941 tgcatgatcg atctccttgt ttgttaagcc ttcgatctca aaagcattat cggactgata
     3001 cgctattatt gaaaggatat cattattatc gtagaacgct ttcagagcga tcgcttataa
     3061 ggaaatcatt atgcgctaca gcaaattgac aatgcttatc ccctgcgcac tgctcctcag
     3121 tgcctgcacc acagtcactc cagcttataa agataacggc acacgcagtg gtccttgcgt
     3181 tgaaggaggc ccggataacg tggcccagca attttatgac taccgcattt tgcaccgcag
     3241 caacgacatc accgccttgc gcccttatct gagcgacaaa ctggcgacac tgcttagcga
     3301 tgcctcccgg gataataacc atcgggaact actgaccaac gatccattct ccagtcgaac
     3361 caccttgcca gacagcgccc acgttgccag tgcatcgact atccctaatc gtgatgcccg
     3421 taacattcca ttgcgcgtgg atctaaaaca gggtgatcag agctggcagg atgaagtgtt
     3481 aatgattcag gaaggccagt gttgggtgat cgacgatgta cgctatttag gcggcagcgt
     3541 tcatgccacg gcgggtacgc tccgtcagtc catcgagaat cgctaacgcc ttgatcaacc
     3601 aataaacccc gtaaattgtc cggcatttcg tgaggaatgc cgacatttat gctcaccgac
     3661 caccgccccc gttattttgt gctatgttta ttgaataatg cgctttgctt ttaactttta
     3721 aagcagaaat attgcataat tattctgtca aaggtactat cttcgacctc aatagctatc
     3781 agactgccag tatacgagtg tcaatgagta ttcaattaaa cggcattaat tgcttctacg
     3841 gcgcgcatca ggcgctgttc gatatcacgc tggattgccc acagggcgaa acgctggtgt
     3901 tacttggccc cagcggcgcg ggtaaaagct cgctgctgcg tgtactcaat ctgcttgaga
     3961 tgccgcgctc cggtacgctc aacattgcag gcaaccattt cgattttacc aaaacaccct
     4021 ctgacaaagc gattcgcgat ttgcgtcgta acgttggcat ggtgtttcag caatacaacc
     4081 tgtggccgca tctgaccgtg cagcaaaacc tgattgaagc gccctgccgt gtactggggt
     4141 tgagtaaaga tcaggcgctg gcccgtgcag aaaaactgct ggaacgtctg cgtctcaaac
     4201 cttatagcga tcgttacccg ctgcatcttt ctggtggtca gcagcagcgt gttgctattg
     4261 cccgtgcgtt gatgatggaa ccgcaggtac tgctgttcga tgaaccgacc gccgcactgg
     4321 acccggaaat tacggcacaa atcgtcagca tcattcgtga gctggcagaa acgaatatta
     4381 cccaggtgat cgtcacccac gaagttgaag tggcgcgtaa aaccgccagc cgagtggtgt
     4441 atatggaaaa tggtcatatc gtagaacaag gcgacgcgag ctgctttacc gagccgcaaa
     4501 ccgaagcatt taaaaactat ctctctcact aagattcggg ataacaacaa tgaaaaaagt
     4561 tctgattgcc gcgttaattg caggttttag tctttccgcc acagctgccg aaaccattcg
     4621 ttttgctacc gaagcctcct atcctccgtt tgaatcgatt gatgcaaaca accagatcgt
     4681 tggttttgac gtcgacctgg cacaggcact gtgtaaagag attgatgcaa cctgtacttt
     4741 ctctaaccag gcgtttgaca gcctgatccc aagcctgaaa ttccgtcgcg tagaagcagt
     4801 gatggcgggc atggatatca ctccggagcg tgaaaagcag gtactgttta ccaccccgta
     4861 ctatgacaac tctgccctgt ttgtgggtca gcaaggcaaa tacaccagtg ttgatcagct
     4921 gaaaggcaaa aaagtcggcg ttcagaacgg gacgacacac cagaaattca ttatggataa
     4981 gcacccggaa atcactaccg ttccgtatga cagctaccag aacgcaaaac tggatctgca
     5041 aaacgggcgt atcgacggcg tcttcggtga caccgcagtg gtcactgagt ggctgaaaga
     5101 taacccgaaa ctggcggcgg tgggcgacaa agtgaccgat aaagattact tcggcactgg
     5161 cctcggcatc gcggtacgtc agggcaacac tgagctgcag cagaaactca acactgcgct
     5221 ggaaaaagtg aagaaagatg gcacttacga aaccatctac aacaaatggt tccagaagta
     5281 attcctgatg aatgaatttt ttcctttagc aagcgccgcc gggatgaccg tcggccttgc
     5341 cgtttgtgca ttgattgtcg ggctggcgct ggcgatgttc tttgcggtat gggagtcggc
     5401 aaaatggcgt cctgtcgcgt gggcaggttc agcgctggta accattctgc gtggcctgcc
     5461 agaaattctg gtggtgctgt ttatctattt tggctcctcg cagctgctgc tgacgctttc
     5521 ggatggcttc actatcaatc ttgggttcgt gcaaatccca gtgcagatgg acattgagaa
     5581 cttcgacgtt agcccgttcc tttgtggtgt catcgctctg tcactgctgt atgccgccta
     5641 tgcctcgcaa acgcttcggg gcgcgttgaa agcggtgccg gtgggtcagt gggagtctgg
     5701 tcaggcgctg gggctgtcga aatcggctat ctttttccgt ctggtgatgc cgcagatgtg
     5761 gcgtcatgcg ctgcctggcc tcggtaacca gtggctggtg ctgctgaaag ataccgcgct
     5821 ggtcagtttg attagtgtga atgatttaat gctgcaaaca aaaagcatcg ctactcgtac
     5881 ccaggaacca tttacctggt acattgtggc ggcggcgatt tacctggtga tcaccctgct
     5941 cagtcagtac attctcaaac gcattgacct gcgcgcgaca cgttttgagc ggaggcccag
     6001 ctaatgtttg agtatttacc cgaactgatg aaagggctac acaccagcct gacgctaacc
     6061 gttgcctcgc tgattgtggc actgattctg gcattgattt ttaccatcat cctgacgctg
     6121 aaaacgccgg tgctggtgtg gctggtgcgg ggttatatca cgctgtttac cggtacgccg
     6181 ctgctggtgc agatcttcct gatttattac gggccgggcc agtttccgac tttgcaggag
     6241 tatccggcac tgtggcattt gttgtcagaa ccgtggttat gtgcgctgat tgcgttatcg
     6301 ctgaacagtg cggcgtatac cacgcagctg ttttacggtg caattcgtgc gatcccagaa
     6361 ggtcagtggc agtcctgtag cgccctggga atgagcaaaa aagatacgct ggcgatcctg
     6421 ctgccgtatg cctttaaacg ctcgctctct tcttattcca acgaagtggt gctggtgttc
     6481 aaaagtacct ctctggctta caccattacg ctgatggaag tgatgggata cagccagttg
     6541 ttgtacggac gcacctacga tgtaatggtg ttcggtgcgg cagggattat ttacctggtc
     6601 gtcaacggcc tgctgacgct gatgatgcgt ctgatcgagc gcaaagcact ggcattcgaa
     6661 cggcgaaatt aatgtagctg cgtacattcg ccgggggggt ttgtaggtct gataagacgc
     6721 gccagcgtcg catcagacat cagcacggtg cctgtgccgg atgcggcgtg gacgccttat
     6781 ccggcctaca tagtgcataa attcttataa tgaagacgga caactcacta aggtatccgt
     6841 ctttttttat ttcatttaaa ttatttaatc atatttattg catataaatt cacttgatgg
     6901 cattgttatc ccatgccgca gacacggcca aaaatcataa gattgacaga cgggagttcc
     6961 atcatgaaaa agttagttct tgccgcttta cttgcttcct ttactttcgg tgcttctgcc
     7021 gcagagaaaa tcaattttgg cgtttcagcc acctatccac cctttgaatc tataggtgct
     7081 aataatgaga ttgtcggctt tgatatcgat ctggcaaaag ccttgtgcaa acaaatgcag
     7141 gcagaatgta cttttactaa tcacgcgttc gacagcctga tcccgtcact gaaattcaga
     7201 aaatatgacg ccgtaatctc cggtatggat atcacgcccg agcgtagcaa gcaggtatcg
     7261 tttaccacgc cctactatga aaactcagcc gtcgtgattg ccaaaaaaga tacctacaaa
     7321 acgtttgccg atctgaaagg caaacgtatt gggatggaaa acggcaccac gcaccagaaa
     7381 tatattcagg atcagcaccc cgaagtgaaa actgtctcct atgacagtta tcagaatgcc
     7441 tttatcgatc tgaaaaatgg tcgtattgat ggggtatttg gtgacacagc ggtggtaaac
     7501 gaatggctga aaaccaatcc gcaactgggc gttgctactg aaaaagtgac cgatccgcaa
     7561 tacttcggca ccggtctggg catcgctgta cgtccggata acaaagctct gctggaaaaa
     7621 ctgaataacg cgctggcagc aattaaagct gacggtacat atcagaaaat caatgaccag
     7681 tggtttccgc agtaattgag actgatgaca aaagtaagat tgcctgatgc gctgcgctta
     7741 tccggcccac atcaactctg caatgtattg atttagcatg attttgtagg ccggataagg
     7801 cgtacacgcc gcatccggca tgaacaaagc gcacctgatc agcaatctga agccgcacct
     7861 gcggcttttt tattgcttca ccagcagcgt cagcacttca tagtgcgcgg tgtgcgggaa
     7921 catatcaaaa agctgtaccc gttcgatgcg gtaacctggc agttcgcgga tatctttcgc
     7981 catggtttgg gcgttacagc tggagtagat aataaaacgc ggtgccattg ttgagagata
     8041 atcacatagc ggtttaccaa tgccgcggcg cggcggatta accagcacca gctccggcac
     8101 ctccccctga gcggtggcaa actgagtgga gtccagcgcc tgaaattgca aacgcgttaa
     8161 gcccagttca gcggctgact gctttgcaca ggcaatggcc tctgatgcaa tttcgatccc
     8221 ggttaactgc atgtcaggcg tcgcgcagtg taaaccaaag ccacccacac cgcagaacag
     8281 atcccacata tgttcaactg gcagctgccg tacccagtcg cgcgcggtag cgtacagctg
     8341 gctggcgacc gccggattag tctggaagaa actttgcgga cggatccaca gcggtacgtc
     8401 attaaaacgc tctgccagcg cctgttgttc agtaaggtag atctccgtct ccccttccat
     8461 aatcgccata tgtaccggct gaatattgac ggtaataact ttcagttgcg gtagttgttc
     8521 ctgtaaccac ggcagcgcct tacgcagttg tgccagtttg gtttcagaac gcagtacaaa
     8581 gcgcagcatc atgccgccat cgctctggct ttcagtcagc agaatgtatt tcagttcgcc
     8641 acgcttacgc gccacgttgt agggtgtcag ccccgcacgg gcgataaacg gttttagcgc
     8701 cgcaaaaacg ggcgcaaatg aggcaggata aagcgggcag tcacaaaggt cttctggtgt
     8761 gccgtcgcga tgcagcatac cgagcagtgg tttttcaacg ctaccactca ccaccatttt
     8821 ggctttatta cgaaaccctt gttccgggcc tgacaccggc gcgcaccatt cctcaaccgg
     8881 aaaatcggcg agcagatttt taagatcggc ggttttagcg gagagttgct ctggaatcgg
     8941 ctgcgttatc cactgacagg aacgacagcg acccgcgtcg taaagtgcgc actgcataca
     9001 ttgaccttca catcatcagg ggcgacgatt atacacatta ttgcaactgg aagaaccgcc
     9061 gactggtcga gggaacgaac agcagcatca ggatgagcat atccggcagc ttctgcagca
     9121 tcaggctatg gaagatttca cgttttgatt caccgggaat gctgaacagc tccggataac
     9181 cgtaccccag cgaagccgcc cacaagtaac ttgcggcggt gatttgtgtc agcagatata
     9241 gccagcgcgc ccagcgacgc cctttcacca gtgaaaacgc acaccagatc tcaatgaaaa
     9301 ccagcaccag actgcttaaa aagaccagcg ttaaactcca ggtttgtacg ctgcgatgaa
     9361 tgaactcgcc aataccgcgc acgcccagcg tattgaaaat catcagcacg tcgaggccac
     9421 ggatcataat aatggcgagc gccgccacct gcaccagcgc aggcacattc aggcgagcat
     9481 gagatgaaga tgttttctta aaaaatccca acgtttcgtc ttccatgaaa acaatgccgc
     9541 ggcatgcgcg gcattatgta gccaggttgg caaattttag tgtcttcagc catgtcttgc
     9601 acgctggata tcgcgtatcc gctgtttttc tgcgcgagcc atcagatacc aggcgataaa
     9661 tccgacaatt ccgaccgcac cgagaattaa cgttgcaagg gcgttgattt ccggattcac
     9721 ccccatccgc acgctggaaa agaccagcat cggtaacgtg gtggctcccg gcccggaaac
     9781 aaagctggcg atcaccagat catcaagcga caaagtaaaa gccagtaacc agccagaaat
     9841 gatcgcgggc atgatcatcg gtagcgtaat gacgaaaaac actttcagcg gcgtcgcacc
     9901 gagatccatc gctgcttctt ctatcgagcg atccagttcc cgcaatcttg aagaaataac
     9961 gaccgccaca taagccgtac aaaaagtgac atgcgccagc cagatggtga gcataccgcg
    10021 gtccgcaggc cagccaatgg catgagcaag cgcgacgaat aataacaaca gcgacaagcc
    10081 cgtgatgaca tctggcatca ccagcggcgc ggtgatcata aaggcaaagc catttgatcc
    10141 gcgaaacctg ccaaagcgca ccagcaccac cgccgcaata gtcccgagga tcgccgccgc
    10201 cgttgccgca caggcagcaa ttgtcaggct taaaccaacc gcactcatca tcgcgtcatc
    10261 gcgcaataac tcaccatacc agcgcgttga ccagccggcc cacaccgtca ccagcttcga
    10321 gctgttaaac gaatagataa ccagcatcag cattggcgcg tagagaaagg taaagcccag
    10381 cagcaaaatc acaatccgcc agggcgaacg aactaccggt aaattattca tccgtgttct
    10441 cccacgcttt tttgctggtg tttgtggaac cacattatcg gcacaattag cagcagcaac
    10501 atgatgatcg ctaccgccga ggccaccggc cagtcgcggt tattgaaaaa ctcttgccat
    10561 agcacgcgcc cgatcatgat gctgtccggg ccaccgagca gttccgggat cacaaactcg
    10621 cccacagccg ggataaacac cagcatcgat ccggcaataa tcccaccttt agtcagcggc
    10681 acgatcacag taaagaacgt tttcagaggt cgtgcaccga gatccagcgc tgcttccacc
    10741 agcgaataat caatacgaat caacgcggta taaatcggca gtaccataaa cggcacgtaa
    10801 gcgtaaacaa tgccgatata aacggccaga ttggtatgca gaatggtcag cggttgatcg
    10861 ataaccccca gccacagcag aaaattattc agcacaccgt tgttttttaa tattcccatc
    10921 caggcataaa cgcggatcag aaacgaggtc cacgacggca ggatcaccag tagtaataag
    10981 atattacggg tcgaaggctt actgtgcgcc accgcccacg ccagcggata gccgatcagt
    11041 aaacagcaaa atgtcgaaat cgcagccacc tgtagcgact ggagataagc atcgaaatag
    11101 agcggatcgt cggtcagttg cagaaaatta ccgagattaa gagtgatgga cagttgcccg
    11161 tcagcccact ccatcagttc ggtataaggt ggaatagcgc gcgccatctc cgccaggctt
    11221 attttaaaga caatcagaaa tggcagcaga aacagcaaga tcaaccagat atatggcaac
    11281 gcaatgacca gtttgcgccc atgcttcatt tgcagctgcg acaaccacag cttaaatccg
    11341 cccggcggtt tcgactgggc agcaggttca agtgtattca tctttgctcc ttaaaccgtc
    11401 agcaccacac agctgtccac ttcccagcac aaacgcactt cgtcgcccca ggtcggtaac
    11461 cctttacgat gacgatgggc gttttgtagc tgggcgctaa tcatctgccc acttttcaga
    11521 cgaacgtgat acaccgaaag atcgccgaga taggcaatgt gtatcacctc ccccaccgcg
    11581 aagttacaac cattggcggg cggctcttcg caaagcatga ttttttccgg gcgcagcgct
    11641 acgtgtaccg gcacgttatc gaccactgaa gcatccgcat cgactttcag tggatgcacc
    11701 agccccggcg aatcaagcac caggccatct tcctgacgct ctttgagtac accttcaaag
    11761 acattaaccg aaccgataaa ttcagcgctg tagcgggtag ttggatgctc gtagatctct
    11821 tccggctcgc caatctggac aaatttcccg cgattcataa tggcgatacg ccccgccatg
    11881 gtcatcgcct cttcctgatc gtgggtgacc atcacacaag tcacaccgac gcgctccaga
    11941 atatccacca cttcaagctg catccggtca cgcagctttt tatccagcgc gcccatcggc
    12001 tcatcgagca gtaatagttt cgggcgtttg gcaaggcttc gggccagggc cacacgctgt
    12061 cgctgaccac cggaaagctg atgcggtttg cgtttggcga actcctgcat gtgcaccagc
    12121 ccaagcatct cgttgacccg ggtggcaatt tccgctttcg gtagtttgtc ctgtttcagg
    12181 ccaaaggcga tgttctgctc cacagtcata tggggaaaca atgcgtaaga ctgaaacatc
    12241 atattgatgg ggcgtagata aggtggcacc tgtgacaaat cgacgccatc aagcataatc
    12301 tgtccggcag agggttgttc aaaacctgct agcatacgca gcagcgtgga tttaccacag
    12361 ccggaagcgc ccagcagcgc gaagatttca cctttgtaga tagtcaggct gacatcatcc
    12421 accgcatgtt gaccatcgta ggatttggtg aggttgcgga tttctaatag cggcgtcagc
    12481 gccttacggg ttttcgcctg cgggcgaggg atagcgtcat tcacggctgc tctccggcat
    12541 tgaatgtgtg caagcgcacc atgatggtgc gaattgccgg cgggtgtggt gcgcccgccc
    12601 ggcatctaag gctgcggatt attttccgct cttcactttg gtccacgcgc gggtgcgcac
    12661 acggtcgatt ttcggatcct gcactttcag agtgaacagc ttcgcacgga catccgcagg
    12721 cggataaata cccgggttat cacggacttc cgcactcacc agcggcgtgg ctgctttatt
    12781 ggcgttggca tagaacacat ggtcggaaat atgcgctact acatccgggc gcagcaggta
    12841 attcaggaac tgataggctt cgtctttgtt tttggcatcc gcaggcatgg cgaagacatc
    12901 aaagaacgcc atcgcccctt cttttggaat cgagaacgag acattaacgc cattcttcgc
    12961 ttccttcgcg cggtttgacg cctgccagac atcgcccgcc cagccaatag cgacgcagat
    13021 atcgccgttt gccaggtcgt taatgtattg agatgaatgg aaataacgaa tgttcgggcg
    13081 cagctttaac agcagatcgg tagccggtcc ggtgtaatca tccgctttag tgctgttggg
    13141 atctttgccg agataattca gcacggtagc aaaaacttct tctggcgcat ccaggaaaga
    13201 gacaccgcag cttttcagtt tttccagatt ttcaggcttg aggatcaagt tccagctatc
    13261 gacgggcgcg ttttcgccca gcaccgcttt aactttatca acgttatagc caatcccggt
    13321 cgtcgcccac atatagggca tagcaaattt attgtcggga tcgtgtttgg cgaccaactt
    13381 cagcagttcc gggtcgagat tcttccactc cggcaatttg cttttatcca gcggctggaa
    13441 aactcccgct gtcagctggc gttcaagaaa gcttgctgac ggcaccacca ggtcaaagcc
    13501 ggtgctcccg gccattaatt tgccttccag tacttcgtta gagtcgaaaa catcgtagac
    13561 gactttaata ccggtttctt tttcaaaatt ggccaccgtg tccggggcga tataatcaga
    13621 ccagttataa atgtggagtg ttttttgttc agccgcgagc gtgccgacag agacggccat
    13681 cagagcaccc gcaaccagac ccgatagcca ttttttattt aaggcggtca tatctctttc
    13741 cttctgaaag ttcgttaaca aacgaacaaa agttgtcaca ctgaaagata tgcaataaac
    13801 gtgcatattt tttatcggcg cggtgtaaga aaagagaact ctctcccggc cagcactgct
    13861 cgctatataa aagcatcgca agaataactt tagccagggt ttgagactat agctcggatt
    13921 aaaaaacggt atttaacaaa tttttatgcg tatttgctct atgtgataaa gccaaatcga
    13981 cacaagagaa gtgaaataat ctttaagaaa aggttaaaaa cagcagataa ttttgcagcg
    14041 cgcagccact gcaccagcga ctgcgtgctc gaaaggagtt atcagtggag gaaatagttt
    14101 tcggtaacgt tgttttgccc ttcatcatca gtcggtaaca gcagttgatg ggcattagct
    14161 tcaagaatga ccatcgaaat ctgctcttcg ctctgacgca cgaaccatgc aaactgctca
    14221 taggttacgc cctgcataac ggataaagac tggcaaacca ccagctttgg cagattatca
    14281 tcctgcatat caagaaatgc tttcacggtc agcgaactgg cattgatggc agataaatcc
    14341 gccgccagcg gcaatacggc tgaaggtcgg acttccgcca tggcagaaaa caggatcgtg
    14401 ttatcgatca gatcgatttt ggcatcaaag acaccgtcga aattctgcat atggggcaga
    14461 tgcagagcct gacagttatc acattcaaaa aaactcatcc ccaggtcatc gagccattga
    14521 cgcagcgtat ccagaccagg aacgaccagc gatgtcataa ttttgtacaa cctctttcga
    14581 taaaaagacc ggcacagctt acgcaaaaag cgcaggcaaa accatgatca gtaatgtgat
    14641 tgcgactaac cacccgtttt caggcaatat tctgtcgtag cgtggcgttc gatccagcgg
    14701 atcattttac ccgcgatgtc gataccggtg gttttttcta ttccttccag ccccggcgac
    14761 gcattcacct ccatcaccaa cggcccgcga ttagcacgca gaatatccac accagcaacg
    14821 tccagcgcca tcgttcgcgc ggcttttatc gcgatttcac gctcctgtgg tgtgatactg
    14881 gcgacgcttg ccgcgccgcc acgatgcaaa ttggaacgaa aatcgccctc tttcgcccgc
    14941 cgttcaatcg cagcgaccac ttcatcgcca acaaccagac agcggatatc gcacccttgc
    15001 gcctctttga tatattcctg caccagaata tgcgcgttca gaccgcggaa agcgtcaatc
    15061 acgctttccg ccgcctgacg cgtctccgcc agcacgacgc caattccctg cgtgccttca
    15121 accagcttga ccaccagcgg cgcaccaccg accatgtcga ttaaatcgct ggtatcatcc
    15181 ggcgaatgcg caatgcccgt gacaggcagg tcgatgccct gacgcgccag cagttgcatg
    15241 gaacgcaatt tgtcacgcgc ccgggcaatg gcgaccgact cattgagcgg atagctcccc
    15301 agcatctcga actggcgcag tgccgccgtc ccataaaagg taatggcggt gccaatacgc
    15361 gggatcactg cgtcaaaatg gggtaacttg cggcctttgt agtgaataga agacgccgca
    15421 ggatttatgt tcatgtagca agaaagcgga tcaagaattt caaccaggtg accgcgctgt
    15481 atagcggctt cacgcagccg cttacacgaa tagagcgttc catcccggga caatatggca
    15541 attttcaccc tgcacctctc tgtcagacct ggtcaaaagc ccgcgtatca tacatcgacg
    15601 tggcggtttt agcgcgtcgc ccaaccctgt ttgtgcaaat aatccagaat aaatgggcgg
    15661 ctttctttaa tgattgttcg gcggatatga tcgctccagg tatcccggcg attattgctg
    15721 ccacgggtga ggtaatattc cgccagttgc tcgtcatact gcgccagtgc gcctttatcc
    15781 agcggttgat agctgttttc atgcaccaaa atggaggccg gtaaacgcgg cttaagatcc
    15841 ggattatccg caggccagcc aaggcacagc ccaaacagcg gcagaacatg ctgcggtaat
    15901 ttaagcagtt tcgtcaccgc ttcaatatta ttgcgcaggc cgccgatata taccccgccc
    15961 aatcccagcg attccgctgc gattaatgca ttctgcgcca tcattgccgt atcaacgaca
    16021 ccgagcaaca gttgttccgc caggccgagc tgagcatccg gacagatctg taaatggcgg
    16081 ttaaagtcgg cacagaacac ccagaactcc gccgcttgcg ctacgtgttt ttgcccgccg
    16141 gtcagcgtca ccagttcttc acgtaacgct ttgtcggtaa tgcgaataat gctactgcac
    16201 tgcaaaaaac tggaactgga cgtcgcacgg gcgctgttaa taatcgcctc acactgcgcg
    16261 tcggaaatgg gttcatcagt gaaatggcga atggagcgat ggccacaaat aagttcaatg
    16321 gttggcgtca ttatcttttt ctctttctga acgtgaatat tgcggtggac ggttcatcag
    16381 ctgtggggca agacgttttg ccacctgaag aataaccacc accgcagcgg gaagcatgag
    16441 caaaacaccg agaaaaatca tcagaatctg cacttctggc cgagaaaatg gctcaggcag
    16501 cgacagggag tcgcttaccg acagcagcgc caccgccagt agcatcattc cgataaattc
    16561 cagtatcaac acgcctttag gcaatttacc gatcgcgcgc atacgcttcc ctctgcaaag
    16621 tgagccttca gtctaaaact tttcactgta ttgtgtttaa cagttatagc ttttagcaat
    16681 taatgcaaca ggttaaacct actttcagcg aatacatttt agcgtgatca ttacaggcat
    16741 aaatctatga ggagagaaat aatgcaaacc gttatttttg gtcgttcggg ttgcccttac
    16801 tgtgtgcgtg caaaagatct ggctgagaaa ttgagcaatg aacgcgatga ttttcagtat
    16861 cagtatgtag atattcgtgc ggaagggatc actaaagaag atctacaaca aaaggcaggt
    16921 aaacccgtag aaaccgtgcc gcagattttt gtcgatcagc aacatatcgg cggctatacc
    16981 gattttgctg catgggtgaa agaaaatctg gacgcctgat cgtctgacaa gccctcgcgt
    17041 tgagggcttt actgattttt tctgtgctgt ggtttaaaca aactactgat aaataagaaa
    17101 cacagtgccc ccagcgcaca ccagaacacc gcgcttagta accatgccag ctcttgccag
    17161 aatgagcgcg tcggtgaaaa aaacagccgc ataatgagca tcgaacaggg tgccgccagc
    17221 attgcgccaa acagaggttt caggacttct ctacgctgtg aaaagaagct ggccactgct
    17281 ccaggaagaa tgaaaaatag caagccgatt tcaggatgcc cggcagcccg aaaagcgcct
    17341 ttcatgtgcg tcgccagaaa aaggcacacc acaatgaaga ggacaaaaca gcagattgcc
    17401 cccgcccaac gttgtttatg tttcactcgt tcctcctgac actgcgtcta tcgaacacat
    17461 ttttcgccag tgtggcgttc agtaagataa agccgcttcg cattccatgc taatataggc
    17521 caacgcaatt catatagccg ttgataccta atgtgattac actagtaaaa tatattgtta
    17581 ctttactatc gtttaggtgc gctgaatgaa tctgcgccct gaattctggt aaaaaacatt
    17641 atcgtaaatt accatttctt tcaacagctt actagtaaac aagaagttag cctccgtgaa
    17701 tataaacgtc gccgaattgt taaatgggaa ttacattctg ttattatttg tggtcctcgc
    17761 gcttgggcta tgtctcggaa agttacgact tggttcgatc caactgggta attccattgg
    17821 cgttttagtc gtatcgctgt tattaggcca acaacatttc agcattaaca ccgatgcgct
    17881 taatcttggc tttatgctgt ttattttctg cgtcggggtc gaagccggac cgaacttttt
    17941 ttccattttt tttcgcgatg ggaaaaatta cctaatgtta gcactggtga tggttggcag
    18001 tgcgctggtg atcgccttag ggttaggtaa gctgtttggc tgggatattg gcctgacggc
    18061 cggtatgtta gcaggctcta tgacgtcgac accggttctg gtcggtgctg gcgatacact
    18121 gcgtcattcc ggcatggaaa gcaggcagct ctcactggca ctggataatc tgagcctcgg
    18181 gtatgcctta acctatttaa tcggtctggt gagtttgatt gttggtgcgc gttacttgcc
    18241 gaaattgcag catcaggact tacagaccag cgcccagcaa atcgcccgcg aacgtggcct
    18301 ggacactgat gccaaccgta aggtttattt accggtgatc cgcgcctatc gcgtcggccc
    18361 ggagctggtg gcctggaccg acggcaaaaa tctgcgtgaa ctgggtattt atcgacaaac
    18421 cggctgctac attgaacgta ttcgacgtaa cgggattctg gcaaatccag acggtgatgc
    18481 cgtgctacaa atgggcgatg aaatagcgtt ggtaggctat cccgacgccc atgcccgact
    18541 cgatcccagc ttccgtaacg gtaaagaagt tttcgatcgt gaccttctcg acatgcgtat
    18601 cgtcactgaa gaagtggtcg ttaaaaacca taacgctgta ggtaaacgtc tcgcacaact
    18661 gaagttgacc gatcacggtt gcttccttaa ccgcgtcatt cgtagccaga ttgagatgcc
    18721 gatagatgac aacgtcgtgc ttaacaaagg tgacgtttta caagtcagcg gtgatgcccg
    18781 tcgcgtaaaa accatcgccg atcgcatcgg ctttatctcg attcacagcc aggtcactga
    18841 cctgctggca ttttgcgcct tctttgttat tgggctgatg atcgggatga tcaccttcca
    18901 gttcagcaca ttcagtttcg gcatggggaa cgctgccggg ttgttattcg ccggaattat
    18961 gctgggcttt atgcgtgcta accacccgac cttcggttac attccgcaag gtgcattaag
    19021 catggtgaaa gagttcggct tgatggtgtt tatggcaggc gttggtctga gcgccggtag
    19081 cggtattaat aacggcctgg gcgcgattgg cggtcagatg ttgattgccg gattaattgt
    19141 cagtcttgtg cccgtggtta tctgtttctt gttcggtgct tatgtattgc gaatgaaccg
    19201 cgcactgttg ttcggcgcaa tgatgggcgc acgcacctgc gcgccggcaa tggagatcat
    19261 cagtgataca gctcgcagta acatccctgc gctgggctat gcgggcacct acgcaatcgc
    19321 caacgtcctg ctgacgctgg cagggacaat catcgtcatg gtatggccag gattaggata
    19381 aaactgaagt tgccctgaaa atgaaatttt tttgcacaac cgcagaactt ttccgcaggg
    19441 catcagtctt aattagtgcc actgcttttc tttgatgtcc ccattttgtg gagcccatca
    19501 accccgccat ttcggttcaa ggttgatggg ttttttgtta tctaaaactt atctacaacc
    19561 ctgcaaccct ctcaaccatc ctcaaaatct cctcgcgcga tagcggctta cggtcggtga
    19621 caaaatgcag cgtcatcccc tcaataaacg catcaagcgc gcgggcggtt ccgggttcaa
    19681 accattgttc gagcgtttgc tgactgcgct gcatccagtt ttgcattacc gtttttaata
    19741 gcggttttcg gctagccagc gcgtagagct ggtacatcag ctccatatta tccggcgttg
    19801 caacctgtga gctgtagatc atatcggtga tagcctggca tgcgccttga gcatcactaa
    19861 catcgctaaa aaatgcctga tattgccggg acataatctc agtaaaactg ctgaacgcct
    19921 ccagcaacaa ctcatcaatt cctgaaaaat agtaggtcat cgatcccaac ggtaccccgg
    19981 caagggtagc aattttgcgg tgcgtaacag catgtattcc gtaaagtttc accgcctcca
    20041 gcgtggcctg gataattttt tctcgccgtt gcggatcgtt agcgcgacgc atgtcatttc
    20101 ctctctctgc aattgtgtac aaatgtacac aaccttgcta atgttgtctt ccctcttctt
    20161 attttgacgt gatctatgac cgtaaattct tcacgtaatg cattgaaacg ccgaacctgg
    20221 gcgctgttta tgttcttctt tttgccaggc ctgttaatgg cgtcctgggc aacccgtacg
    20281 cctgctatcc gcgatattct ctctgtctcg atcgctgaaa tgggcggtgt tctctttggt
    20341 ctgtcgatcg gttcgatgag cggtattctc tgctcggcgt ggttagtgaa acgctttggc
    20401 acgcgtaatg tcatcctggt cacgatgtcc tgcgcattga tcgggatgat gatattaagt
    20461 ctggcactct ggctgacatc gcccctgctc tttgccgttg gtctcggcgt ctttggggca
    20521 agttttggtt ctgcggaagt ggcgataaac gttgaaggtg ctgccgttga gcgagaaatg
    20581 aataagacgg ttttgccgat gatgcacggt ttttatagcc tgggcacgct ggcaggcgca
    20641 ggtgtcggga tggcactgac ggcctttggc gttccggcaa cggtgcacat tttattggcg
    20701 gcgctggtag gtatcgcacc tatttatatc gccattcagg caatccctga cggtacgggc
    20761 aaaaatgctg ccgatggcac ccagcatggc gaaaaaggcg taccttttta tcgcgatatc
    20821 cagttgctgc tgataggtgt tgtggtgctg gcgatggcct ttgccgaagg ttctgccaac
    20881 gactggttac ccttattaat ggttgacggt cacggtttta gccctacttc cggctcgctg
    20941 atttatgccg gttttaccct ggggatgacc gttggacgct tcaccggcgg ttggttcatc
    21001 gaccgttaca gtcgcgttgc cgtggttcgg gccagtgcac taatgggggc gttgggtatt
    21061 gggctgatta tttttatcga tagcgcctgg gtcgctgggg tgtctgttgt actctgggga
    21121 ctgggtgcct cgctgggctt cccgctgacc atttctgccg ccagcgatac cggccccgat
    21181 gcaccaaccc gcgtcagtgt ggtagcaacg accggttatc tggctttcct cgtcgggccg
    21241 ccgctgctgg gctatctcgg cgaacattat ggattacgta gtgcaatgct ggttgtactg
    21301 gcgctggtta ttctcgcggc tattgtcgcg aaagccgtcg ccaaacccga taccaaaacg
    21361 cagacggcga tggagaatag ttgatgagca ttaaattaat tgcggtagac atggatggta
    21421 ctttcttaag cgatcaaaaa acctataacc gtgagcggtt tatggctcag tatcagcaaa
    21481 tgaaagcaca aggcattcgc tttgtggtcg ccagcgggaa tcaatattat cagttgatct
    21541 ctttcttccc tgaaattgcg aatgaaattg cctttgtggc tgaaaacggc ggctgggtag
    21601 tgagtgaagg caaagatgtt tttaatggcg agctgtcgaa ggatgccttt actactgtcg
    21661 tggaacattt gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa aatagcgcct
    21721 atacgctcaa aaaatatgac gatgccatga aaacggtggc ggaaatgtat tatcaccgtc
    21781 tggaatacgt cgataacttt gacaacttag aagatatttt ctttaagttt ggcctgaatc
    21841 tttccgatga actgattcca caagtacaaa aagcattaca tgaggccatc ggcgatatta
    21901 tggtgccagt ccacaccggc aacggcagca tcgatctgat tatccccggc gtacataaag
    21961 ccaatggcct tcgccaactg cagaaattat ggggaataga cgacagcgaa gtggtggtct
    22021 ttggcgatgg cggtaacgat attgagatgc tgcgtcaggc aggctttagt tttgcaatgg
    22081 aaaatgccgg cagcgcggtc gtcgcagcgg caaaataccg ggcaggctcc aataaccgtg
    22141 aaggcgtact ggatgtgatc gataaagtgc ttaaacacga agcgccattt gaccaataaa
    22201 aacggcagca cgcccggcaa cacaatacct gaatgaaggc tgcataattc gcggaaatta
    22261 acccgctgca ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt gggcgcgctt
    22321 ttaatgggct ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt gccgagtgga
    22381 tacaaagtgg ttgtggttcc tgattcagtc agcgttccac aagcggtcag cgtcgcgact
    22441 gtgccgcaaa ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc gcatccttat
    22501 gttcgccatc ttgccagcgt aggtgaaggg atggttatcg aacaccagat tgacgaccat
    22561 caccattaat tcgagaatgc ctgatcgcac aaagcaatca ggcatttttt tacccttcgt
    22621 gagaatttcc catctgttta tcttttaaaa agataaccat cagcgacagc cacaaaattc
    22681 cgttgacaag gttgaagaga ttaaactgtc cgttgccccc gttcagccag gcatgtttgc
    22741 tgatttcaat accaacggta aagatcagca tttgcagcat tcccatcgcc gcagaaaccg
    22801 tacctttact catatcgctg gcaaacaggg ttaatcgcac cagtcccgca ttcgccagac
    22861 caataccgaa agcataaata cttaacccgg cagtcatcca taaatacgcg tgcgatgaga
    22921 taaccgttgc cgcagcagcg accaacagac caatcataat cggccagccg cccataatta
    22981 tcagcgaacg tacggtgcgg cgcgaggtca aacgcgctaa cagcaagtta cccgcaatta
    23041 acgccccgaa aataggcact tgcagcaagc catattcata gctgctcaac tgctcgccgg
    23101 taatgatgat aatcggcgac tgggcgatcc acgccagcaa tggcaggcta acgaatccca
    23161 gcgccagcgc ccccgccaca aagcggccgt tcttcagcac cagcttatag tcacgaccga
    23221 gttctttcag tgacagtttc tcgcctatac gcgtggcggt ttcaggcatg gctcgttgca
    23281 gaccgaaaaa ggagatcgct gccaatgcgg caaacaaaac aaacatcccc tcccagggca
    23341 gcacatggat ccacgccgcg cccaccagcg gaccaagtag cggagcaatc agcgccacgt
    23401 tcgccatcag cgcggtgatc ttgatacaaa ccgcctcttc gaaggattcc tgaattgcgg
    23461 cgtatcccac agcgccaatg aaacagaggc ttatgccctg caagaagcgc aacagggtga
    23521 attgttcaat gttttgcgcc agcaatattg ccagacaggt gatgataaac cacaccaccc
    23581 ccgccagcat caccggacgg cgaccaatac gatccgacag cggccccagg agccattgta
    23641 aaaacatccc gcccgccaga tatgcggtca ttgaagtagg aacccaatca atgcccgcct
    23701 gatattgttc caccacggcc aacataccgg gttgaatcat atcgttgccg atataggttg
    23761 aaaattcgta aagcaccaga cagagaggga aaagtaacgc ctgacgtcca agcctggcac
    23821 cggaagctaa tttattttgc atgcaatttc ttcgccaata ataatcgcgc agagtttaat
    23881 aaaagcacag ctaacgagaa agcgaatttt gtagctgaaa ccacggttaa gcacattctt
    23941 acattattgc gagtatagct acgctttctt taagttttat ttaacctatg cccgttacaa
    24001 ttacccaccg taaacaggcc gcttgaggga aataagacga tgccgcttta cccagtttaa
    24061 cctgcacttt attctcaacg acttgcctgt attggctccc ttttaatcac tttgcgtcgg
    24121 gaagttatgc tggaaaattt gaatctctct ctattctctc ttattaacgc gacgccagac
    24181 tcggctccgt ggatgatctc gttggcgatt tttattgcta aagatttgat taccgtggta
    24241 ccgctgctgg ccgcggtgct ttggttgtgg gggtttacag cacaacggca actggtgata
    24301 aaaatcgcta tcgcgctggc ggtcagcctg tttgtgtcct ggacgatggg acatcttttt
    24361 ccgcacgacc gaccctttgt cgaaaatatc ggctataact tcctgcatca tgcggcggat
    24421 gactcattcc caagcgatca cggtacggtg attttcacct ttgcactggc atttttatgc
    24481 tggcatcgcc tgtggtccgg ctcactttta atggtgctgg ccgtcgtcat tgcctggtcg
    24541 cgcgtttatc ttggcgtcca ctggccgctg gatatgctcg gtggattgct ggcaggtatg
    24601 attggctgcc ttagtgccca gattatctgg caagcgatgg ggcataaact ctatcaacgt
    24661 ctgcaatcgt ggtatcgcgt ctgttttgca ttaccgatcc gcaaaggctg ggtgcgtgac
    24721 tgatctctgc taaaaagtgt agtattgagc ggctcgcttc aataactatt cagagggatt
    24781 atggaaacac gtcgcgaaga gcgtatcggg cagctgctgc aagaattaaa acgcagcgat
    24841 aagttacatc ttaaagacgc cgccgccctg cttggggttt cggagatgac gattcgtcgc
    24901 gatctgaaca accacagtgc gcccgtcgtt ttgctcggcg gctatattgt tctggaaccg
    24961 cgcagtgcca gccattacct gttaagcgat caaaaatccc gcctggtgga agaaaaacgc
    25021 cgggcggcaa aactggctgc gacactggta gaacccgatc agaccctctt ttttgactgt
    25081 ggcaccacca cgccgtggat tattgaagcg attgataatg aaatcccttt taccgccgtt
    25141 tgttattcgc taaatacctt tctggcgctg aaagagaaac cccattgccg cgcatttctg
    25201 tgcggtggtg aatttcacgc cagcaacgcc attttcaaac ccatcgattt tcagcaaacg
    25261 ctgaataatt tttgcccgga tatcgctttt tattctgcgg cgggcgtgca tgtcagtaaa
    25321 ggcgctacct gttttaatct tgaagagttg ccggtaaaac actgggccat gtcgatggcg
    25381 caaaagcatg tgctggttgt cgaccacagt aaatttggca aggtgcgtcc ggcgcgcatg
    25441 ggtgacctga aacgctttga tattgtggtg agcgattgtt gcccggaaga tgagtatgtg
    25501 aagtacgcgc agacgcagcg cattaagttg atgtattaat gacgtataac cggatgacgt
    25561 ttcgcgccat ccggttatca gaagattaag agaaccagct gccgaaccac tgatggaatt
    25621 tcatcatcac gaaatcccac atccgaccaa agaatccgcc ctcttccaca ttttccatca
    25681 cgatcagcgg acgctgctca atggatttac cgttaagctg gaaatcaatg gtcccgacaa
    25741 cctgaccttt tttcagcggt gcggtaagct gcggttccgt taacgtataa ctcgctttca
    25801 ggtttttcag ctgcccacgc ggtatggtca ctgagcccgc ttcacctgcc ccgagattca
    25861 cttcgctctt atcaccaaac cagacgcgct gagtcacaaa ggtggcatca ggtttaattg
    25921 gcgtcacggt ttcaaagaag cggaaacccc aggtcaataa tttctcagac tcattaaaac
    25981 ggatacggtc agttttcgcc cccagcacta cggagattaa acgcatatcg ccctgggtag
    26041 ccgaagcaac cagattatat cccgcgcctg ccgtggttcc tgtcttcatg ccatcaacat
    26101 tcagattgct gctccacagc agacggttac ggttaggctg acgaattttg ttgaaggtga
    26161 actctttctc tttatgaatg gcgtactctt ccggcacatc gtggatcaat gctttaccca
    26221 gcaatgccat atcgcgcgcg gtactgaact gccccggcgc atccagaccg tgcaccgtct
    26281 ggaaggtagt gttggtcaga cccagttttt tggcataacc attcatcaaa ccaatgaatg
    26341 actcctggct cccggcaacg taatcggcca gcgcaataca ggcgtcatta ccggactgga
    26401 taatcacacc tttgttcaag tctgccaccg aaacctgatc gcccggtttg aggaacatta
    26461 ccgatgaacc acgcagtgcc ggatttcccg tcgcccaggc atctttaccg accgtcacca
    26521 tatcggtgag tttaatctta tcggccttaa gcgcctgccc aaccacatag ctggtcatga
    26581 ttttagtcag gctcgcggga tccagtttct catccgcgtt gccttctgcc agcactttac
    26641 cgctggcgta atccattaaa atccatgcac gcgcatccac gctcggcgct tcaacggttt
    26701 gttccgccgc gaatgccgtt ggggcaaaaa ggaataaaaa tgcagaaccc gctgcaagac
    26761 cacgaaggag agaggagtat tgcgtcataa atgccacccg agtatccatt caaaaaaaat
    26821 acgttatatc gccgtatgat aagttcggtg attaataacg cacacacaaa cttaaagaaa
    26881 ccgtaagttg gtaaagtttt taaagtttat gcaatatcct gccgcttcgc tgcttctggt
    26941 gtcatttttt tgccagctgt tgcttaaaag ttaactttac ttcaccatcg gagtcctgtt
    27001 cagcctgtgg ctgcctgtcg gaaaaggggt gagtatgatt acgctgtggg gtcggaataa
    27061 ttcaaccaac gtaaaaaaag tattgctgac gctcgaagaa ctggaactac cttatgagca
    27121 aattctcgcg ggccgtgagt ttgggataaa tcacgatgct gattttctgg cgatgaaccc
    27181 taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat ctcattcttt gggaatcaaa
    27241 cgccattgtc cgctatctgg cggcacagta cgggcaaaaa cgtctgtgga tcgactcacc
    27301 ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca aaccagacgc tcagtaatgc
    27361 tcatcgcggg atcctgatgg gattagtcag aacaccaccg gaagagcgcg atcaggccgc
    27421 cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc ctgctcgatg cggaactggc
    27481 aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc gatatcgcta tcgcaccgtt
    27541 catctacaat ttgtttaacg ttggcctcac ctggacaccg cgtccaaatc tgcaacgctg
    27601 gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg gtgatgattc ccgttagcta
    27661 attgcgtggg ctaactttaa gtaattcccc actggactcg tcggtgagaa cgtataaata
    27721 accgtcgggt ccagtgcgaa catcacgaat tcgctgccct ctgtccgtta aaatacggcc
    27781 atcttctgtc actttgtcgc cgttgacgct catcacaatg acatctttat ctttcagcgc
    27841 gccaataaat aatttttgct gccactgggg gaatttatcg ctgttataga aggccatgcc
    27901 gctcacggcg ggcgaatctt tccagtaaaa aacaggttgc tcggtcccgg cgacgatctc
    27961 ccctttcgct tccggtatct taaagcctga atagttgatt ccccaggttg ccagcggcca
    28021 gccgtagttt ttgccttttt gcgggatatt aatttcatca ccaccgcgcg ggccatgttc
    28081 attcagccat agtgcattac tccacggatt catcgccatt ccttgcggat tacgaatgcc
    28141 ataagaccag atctcggcgc gcgcaccgga ttcctttata aaaggattat catccgggat
    28201 ttcgccctgg tcggtcagac gcaccagttt gccctgtaat ttatccagat cctgcgccgt
    28261 cgggcgctga ttgttttcgc ccagagcaac aaaaagataa cctttaccgt cgaataccag
    28321 ccgcccgcca aaatggttgc cggtagacag ttttggcatc tggcgaaaga cggtgcggaa
    28381 gtcggtcact tttgagagat catcacttaa gcggccataa cccacagcag ttccggcttt
    28441 gccatcatcg ccaacttcgg aataacttaa ccagatgcgg cgagactgag caaaatcagg
    28501 cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc caaacgtccg gaactccgga
    28561 aagcggcgca gataatcctt ttcctgcttg ccagtgacgc aactcgccgc cgcgcagagt
    28621 gattaacata ccgtgattat cgggtaaaaa ggccagtgcc cagggatggt cgagtttgtc
    28681 ttgcagtact tcgacattta ccgttgcagg agccgcccag agagcggaag aaagacaaat
    28741 aaggggcaca aggaaaaagg attgtcgatg cataacgcct ccggattgac tctggcttaa
    28801 agcgtagtca gtggaggaga taacaaattc atttttacaa aaacttaaac atgaaggggg
    28861 agacgctttc tcccccttag ttttcaggcc ttctcaagca tggcgtgctt ctgcaggctc
    28921 tggatactca gcgttaagct catcagacaa ttttcaagct tatcggcgtt gacggtaata
    28981 acagtcgggc aatcatggtg cccactcatc aaacatactg cggctgtcgc taatgcttct
    29041 tcagcatgat gaagagcact ccactcttcc tgatccagat gaagattcaa ccgcagcgat
    29101 ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc gtagatgatt gctttcgctg
    29161 acggacatgt atccttttgc cttcctcagt tgcacgtatg cagcgaggtc aacgcgtgcg
    29221 tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa cgaacatact tcgatcctcc
    29281 tctttccagc cttgttgcaa ttaaatagta tggctatttt ttgagcaaaa tgctgtccag
    29341 gatcaattct tcacgccgaa ttaacaaaat ttacatttcc ttcgcctccg ccagcgaatg
    29401 agattgcgat acaaatttta tccagaactc tgtagaatcc ctgccctgac ctttcctgta
    29461 actgaatatt ttttaagcta tgagcaaagt aactccccag ccgaaaatcg gctttgtttc
    29521 ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt ctcaccgaac tacgcactga
    29581 aggttatgac gtggtaccga gctatgacga tgcggacatg gtgatcgtca acacctgcgg
    29641 ctttattgac agcgcggtac aagaatcact ggaagccatt ggtgaagcgt tgaatgaaaa
    29701 cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa gatcagatcc gcgaagtcca
    29761 cccgaaagtg ctggaaatca ccgggcctca tagctatgag caggttctgg agcacgttca
    29821 tcactacgtg ccaaaaccga aacacaaccc attcctgagc ctggtgccag aacaaggtgt
    29881 gaaactgacg ccgcgtcatt atgcctatct gaaaatttcg gaaggctgta atcaccgctg
    29941 caccttctgc attattccgt ctatgcgcgg cgacctggtg agccgtccga ttggcgaagt
    30001 gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa gagattctgg tgatctcgca
    30061 ggatacttcc gcctatggcg ttgatgttaa acatcgtact ggcttccaca acggcgagcc
    30121 ggtaaaaacc agcatggtca gcctgtgcga acagttatcg aaactgggga tctggacacg
    30181 tctgcactac gtttaccctt atccgcatgt ggacgacgtc atcccactga tggcagaagg
    30241 caaaatcctg ccgtatctgg acattccgtt gcagcacgcc agcccgcgca ttctcaaact
    30301 gatgaagcgt ccgggttctg tagatcgcca actggcgcgc atcaaacagt ggcgcgaaat
    30361 ctgcccggaa ctgaccctac gctcaacctt tattgtcggc ttccctggcg agacagaaga
    30421 agatttccag atgctactag acttcctgaa agaagcgcgt ctggatcgcg ttggctgctt
    30481 taaatacagc ccggttgaag gtgcagacgc caatgccctg cctgaccagg ttccggaaga
    30541 agttaaagaa gaacgctgga accgtttcat gcagctgcag cagcagattt ccgccgagcg
    30601 cctgcaagag aaagtgggcc gtgaaattct ggtgattatc gacgaagtgg acgaagaagg
    30661 cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac ggcgcggttt atctcaatgg
    30721 tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa gtcgagcacg ccgatgagta
    30781 cgatttgtgg ggtagccggg tttaattaat tcaggccggg caaaatcatt tgtccggcct
    30841 gcgaataatg attactgcca gctaatctga gccctgccgt ttttcttact cttatacagc
    30901 cgctcatccg ccagcatttt caagcgttcg acggtggtac attcatttga atgcgctaca
    30961 ccgatactgg tacttaaatt aatgcttccc acctggaaat gaacgatttc ttttagaatc
    31021 gatagcaact cttccagcgt tccacccgta taaatcaccg caaatttgtc accgccgaag
    31081 cggtagattt tacctttatc taccgggatc tgtgatttaa ggattttcac cactttcatc
    31141 aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa tgtttttgaa tttatcgacg
    31201 tcaaacaggc acaaataacc gcctttttcc ggcagcgctt ttaaatcttc gtcaaaggat
    31261 tttctgccca ttgctttggt caggtcgtcc aggaacgccg agttatacat actgtagagt
    31321 tttttgacat cggcagccga aaagaaaagc ccgccatcca gtacgcgttc aaaatggtta
    31381 ttaatgatgg cttctttctc cacaatacca cgattaatca ctctgcgtac cagatagagg
    31441 caactggtac ccgatatcac tgccaccaat gcggcaaaga aaataatcaa tgtacagacc
    31501 cgcaaataaa catctttttt atacgcaaat acgtacaatc cggtcggcaa ataggcgaag
    31561 tgctccatcg cttccgaaac ggtgaagatg gcgtttttag cattatattc atcaccggtc
    31621 accggtttac cgttcttaaa tagagagaac atcaggttct cgctgtccat cgccagcgat
    31681 tttgacagta aagaaaaacg ctccagacca agcaaaaata tcagactacg attgtgagtt
    31741 accggataga ggtaatagac gaaggtttta ccgtcaaaag agataaaccc tgtatttttg
    31801 acatgtcgat acttcacaac ctgcataata tctgcacaat aatttttctc gaactccttt
    31861 acctgaatac attcgtcact ggaaatcaga taaggaccga catcgtacaa ataagagggg
    31921 gaaaccgagt cggcgtaagc tgtgcgcaaa ccaaccaaca gctgttcctc cgtgggattt
    31981 agcgtgttga taagatcaat tgccacgcta cgccgatatt cagcaatatt tgccaccgca
    32041 aaagcagtat caataacgga ctgtttcagg ctatacaccc gttcctttac catttgagtg
    32101 tagatcccgc aggcaactgc tgaaatcatg ataaaaatca gcagactgac tgtaagtacg
    32161 aacttattga ttctggacat acgtaaacta ctcttttact aattttccac ttttatccca
    32221 ggcggagaat ggcatgggtt tctggtacag atacccctga gcctgaacac atcctaattt
    32281 aattaattcg cgcgcaacgt cttcgctctc tacgccttcc gcaatcaccg ggatccggtt
    32341 atagcgtgcc agatcgaaaa taatgcgcac aatttttccc gcagtaccga aaatatcgcc
    32401 ggtaaattgc gagtccacct ttatgctgtc tggattaatt tcataaaaac gtttcaggtt
    32461 tgacaaaccg gtgccaaagt catcaatcga tatccgtacc ccttgtttac gcagttgttc
    32521 aatattgaga taaaaatgtt cattaattcg ctcgatcacg gtttcagtga tctcaacaca
    32581 gaggctgttc gccttcaggc cattttcttt catgatggtg gtaaaacgct catgaaagtg
    32641 cggttcgttt aactgccagg gcgaaacatt aatatgcagt aagaaatcat ccttcagcga
    32701 cgcacgctga ctaaaatgtc tgaattcacc cattgccgtt ttaataatct gataacccag
    32761 ctcattgata aacccgatat cttcagcaat ggtaatgaac ttcagcggtg aaatgatccc
    32821 ccgctccgca gaaacccaac ggcacaacgc ctccgcgccc agaatggcgc gggtgttaat
    32881 atcaacgatc ggttgataga cgaggaatag ttcgccaatt tccattgcat ggcgaatatt
    32941 acgcgcaatc cagatattct cttctatttc gttgagtttc gcgtcatcac agaaaataaa
    33001 ctcggagaac atatggtcac ggatactgga aatcgccagt ttcagttttt ctgcgtaact
    33061 ccaggcgttt tccccctgca aaggttcaca aatggcagac ttatggatgc gcagattgcg
    33121 gtagccaaag ctgctgtcaa aatcgtttac catcgctatt ttacgggtaa atgtctgtac
    33181 attttcccga cacagaagta aatagagatc ggcattgaat gtcacgatat cgcagcattg
    33241 cgcatatttc tcggcaattc gctgacacaa tttcctgacg attttattgc gttcatcaac
    33301 accaaacacc gcttccacgg cttccagacc acacagtttg atgatcccca cagccagata
    33361 ctgattattg ttggactcaa taatcgccat atttttaata aaaccgcttt cctgatcgta
    33421 agagatcagt gagagcaatt tatttgaccg ttcaatttcg gcatctttca tttccgcgat
    33481 cgaatttttg atgctgataa tctctttcgc atcgccttcg cgaattttca gagcctcgat
    33541 ttgatccgtt gagtcgggca aaaacgccag cgtattaacc agctcggtca tttcagaaaa
    33601 ttgccgacgg aacacacgag tggtcatgaa atataccacc agcaacacga taataatgaa
    33661 agcaaacggt agataaaaaa gtttatctga tgcgttcaat aaattaccgc caggataata
    33721 aaaatagatt tcgaacaatg cattatttgg cactgctgat gtctttgcaa tatactccgt
    33781 atcgacagaa tcaataatat tataattacc ttcgctatta taaataatca gtttttcacg
    33841 agcgagttga ttcttggcaa tgatctcatg accttgtttc aaaaatagaa cttcatcatt
    33901 agcgctgatt tgatcaagat actcgttcag ataccgccag tttaaatcga catgaatttt
    33961 atctaccgct aacaaatcgt gatctttgcc gttgatacgt agcgtaaaag agatacattt
    34021 tttacccgga cgatcaaagc aattgtatgc cggattccag ctgttgttag agtcatctga
    34081 agccaccgac cacggtcgtt tcgatgggtt aaaatcggca aaaataaccc gccctgactc
    34141 gtcgacaagc ggtccattta cttgttcctg atgataaaca acgattttat cattcgccct
    34201 ccgcgaaaag agataatatt tattatcatc aagtaccagt cccaccgagt ctaaaaatgg
    34261 catcatttcc attttcagaa tcacggcgcg ttgaatctcc tctgtatttt ttaataaata
    34321 cagtgagtcc tttaatgtca gggccgaacg ttcaatatct ttaaagaaat agccaacgtt
    34381 tttctgaata agttcaaagc ggctatcggc acgttttacc gcgcgttccg ataccagttg
    34441 atagttaatg atgaaaaaaa gaccaataaa tgacagtgct gccaataaaa ataaaattat
    34501 cagccttatc tttatctttt cgtataaact cagcatgtta atacatttaa caaaataact
    34561 atctgaacgc tgtgccgctg caaaaatagt cccttgctgc ggctttttaa ccaaattacc
    34621 cgattctaca aaatcagcag aaagttacaa cacttgctta gggcaataat tgcctgtgag
    34681 gtgattaccc tttcaagcaa tattcaaacg taactatcct ttaattttcg gatccagcgc
    34741 atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg gtcagaaaaa tagccagggc
    34801 cggaaaaaca gcgacatgcg gcgcgataac catatccgct cgtgcctcat tgagcattgc
    34861 tccccactct ggtgtcggcg gctgcgcacc gaggccgaga aatgacaggc tggcggcaga
    34921 gataatcgag gtgccaatgc gcatggtgaa aaacaccacg atagacgaga cggtcccagg
    34981 caagatatga cgcaataaaa tggtcatatc gctggcacca atactgcgtg ctgactcaat
    35041 aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc aggcgggcaa acgcggggat
    35101 ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg cttcccaaca ccgcaacaac
    35161 agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca tcgcaaatgc gcatgatcag
    35221 ccgatcccac cagccttcat aatatccagc cagcaagccc agcaacgtcc cgatcgccgc
    35281 accgataaac acggcaaaca cgcccgccgc cagcgagatt tgcgcaccaa ccaggacacg
    35341 gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag tgctgcaaag aaggtccgtt
    35401 attcagattg tcataatcaa aataattttc ggcgtcatag ggagcgatcc agcgtgcaaa
    35461 aatggccacc acaatcaata aaataacgaa taatgcggcg gtcatcgcca tatgctgacg
    35521 gcgaaatcgt cgccagaatt catgccacgg tgtacgcacc tggtcaggtt tgaccagtgg
    35581 catggcgttt aacaccgcct gacgtcgcca gttaaatagt cgcatcctta cttgtacctg
    35641 atagccgggt taatggcggc gtaaagcaca tccaccacta agttgataag aataaattcc
    35701 agcgagaaaa gcagaatttc cgcctgaatc accgggtaat cgcgcatttc tacggagtca
    35761 acgagtaagc gtccaagtcc gggccagttg aaaactttct ccaccacgat agaaccaccg
    35821 agcaaaaagc caaactgtaa gcccatcatg gtcactaccg ggatcatcgc gttacgtagc
    35881 ccgtgtttga ggacaaccca ggtttcgctc acccctttcg ccctcgcggt acgcatataa
    35941 tcttcgctta aaacatcgac aaacgacgcg cgggtaaagc gcgccatcac ggctgccact
    36001 gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc aactgtctgc tcccacggta
    36061 ggcagccagc ccagttcaac ggagaatacc tgaattaaaa gcatccccag cgcaaatgcc
    36121 ggaaacgaga tccccgacac cgcaatggtc atactcaatc gatccggcca acgattacgc
    36181 cagacggcgg cgataattcc cgccgccata ccaaatatca ccgcccagac catactggtt
    36241 atggtcagcc acagcgttgg cataaagcgg ctggcaatct catcggcaac cggacgacgc
    36301 gacaccatcg acaggccaaa atccccctgc acagcattgc tgatatagtg ccagaactgg
    36361 tgatacagcg gctgatccaa ccccagctgc tgacgcacca gttctataac ctgcgcatca
    36421 gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca gcatatgcac aaataaaaac
    36481 accagcaccg agacgataaa cagcgtcgga atcaacccca gtaatcgttt gataacgtaa
    36541 ttaagcattc cactccctgc gttgcttatt gcaaatccgc gtcttcaaag ctgaagccgg
    36601 tgtctggcat gatccaaaaa ccggtcaggt ttttactgtg tgccgacacc agtttttcta
    36661 ccaccagcgg gatccacggc gactcttgcc agatgatatc ctgcgccgcc ttatacaagc
    36721 gggtcttttc cgccggatca ttagttttca gtgcctgagc caggaagtcg tccacctgtt
    36781 tattgctgta aaatgcggta ttaaacagcg tcggtggcca gttttgcgag gcgaacagtg
    36841 gcgatagcgc ccagtccgct tcgccggttg aagccgacca gccagtgtag aacatccgca
    36901 cgccgctctc tttttgccct ttaccttcaa cttctgccgc ccgctgtccg gcatccatcg
    36961 cggtcacctg ggctttaatc ccgacctgcg ctaactgctg ctgggtaaat tgcagcactt
    37021 tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt actgaaaccg ttgggatatc
    37081 ccgcctcttt cagtaattcg cgcgctttca ctggatcgta aggccacggt ttataacttt
    37141 gcgcgtaggc gatacttggc ggtaccacac cagtagctgg cgttgcatag cccgcaaagg
    37201 caactttcac cagcgccgga cggttaatgg cgtaattcag cgcctcacgg accttcgggt
    37261 tatcgaacgg cttttgcgtc acgttcatac tgatataacg ctgcataatt gacggactgg
    37321 ccatcaactc gatatttttg tttttctcca gcagtgtggc cttctcgtag ggaatgggga
    37381 aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg ggtgttgtta tccgccaccg
    37441 gacgccaggt tatgctgtcc agtttgggca atcctggctg ccagtaaccc gcgaattttt
    37501 tcaccttcac aaaatcggtc tgattccagg tatccagttc atacggtccg gttcccaccg
    37561 gatgaaaacc aatctccttg ccatattttt ccagcgctgc cggtgaaatc atcgcggtcg
    37621 ccggatgggc aagaatatta ataaacgctg agaacggctg tttgagagta atctttactg
    37681 ttgttggatc gatcgcctcc gttttggcaa tattcttata caggttatag cgtttaagat
    37741 gattcgccgg atcgctggcc cggtccagat tcgcttttac cgccgctgcg ttgaaatcgg
    37801 tgccatcctg gaatttaatt ccttcccgca atttcacggt gtatgtaagg ccgtcatcgg
    37861 aaacggtata actctccgcc agcacgtttt tcagtttcat ctctttatcc agaccaaaca
    37921 gcccctggta aaacgatttc gctacggcct gagataacgt gtcatttgcg tcatacggat
    37981 cgagcgtggt gaagttcgat cctaccgcca ccaccacatc tttggcagcg aatgcacaag
    38041 atgccatcaa cgctgtcgca atgcccaacg ccactaaccc actacggtgt acagctcttg
    38101 ccattttatt ctccgcctga atgttatcta cgcatgaatg cgtattctga ttgtggttgt
    38161 gcgacgtaat gccccggccc gacgcattgc aacgagacgg ctgccacctc ttcgccacgc
    38221 agatgaatat tgctgggaag atcgtccgac agcagtacac gctgcggtcg ttgtcgggac
    38281 ggttcagcga ccggaactgc cgccagtaat ttacgcgtat aaggatgctg cgggttttcg
    38341 aagaccgcgc gccgtggacc aatttcaaca atttgtccga gatacatcac cgccacacga
    38401 tgactaatcc gctctaccac ggccatatcg tgggagataa acagatacgc aatgccgaaa
    38461 tcacgctgga gatcgagcaa caagttgata atctgcccgc gaatagaaac atccagcgcc
    38521 gagacggctt cgtcggcaat gatcactttc ggattcaatg ccaacgcgcg agcaatgcag
    38581 atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac gccaggcatg ttcaggtaac
    38641 aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg cttctttacc tggcaataaa
    38701 ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg tctgacgtgg gtccagcgaa
    38761 gcgtaagggt cctgaaaaat aaactgaata tcccggcgca atgcctgaag tttgccgggt
    38821 gacaaggtat cgattctctg accgttaaag ataatttcgc cgccctgcga ttcgaccagg
    38881 cgcagcaacg cccgcccggt agtggattta ccgctgccag actcgcccac cagcgatagc
    38941 gtttcgccag gccagagatc aaaactgact ttctcaacgg catgcacttc ccgcgttacg
    39001 cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa gattacgcac tcgtaaaaca
    39061 ggttcgccat ccaccaccgt tttctgctcg atgggggggg cctgtttcgc tggatgttca
    39121 agcgatatca acgggaaacg tcggggataa tctaaccctt tcatcgcacc aagttgcgga
    39181 acagcagcta acagcgcacg ggtgtaagga tgttgcggtg catgaaaaat ctgttcgacg
    39241 gtacccgttt ccaccgcctc gccctgatac atcaccagta cccgatcggc aatctctgcc
    39301 accacgccca tatcgtgagt gataaagata acgcccatcg acatctcttt ttgcaatact
    39361 ttgattaatt gcaggatctg cgcctgaata gtgacatcca gcgcggtggt tggctcatcg
    39421 gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa tcatcactcg ctggcgcatc
    39481 ccgccagaga gttgatgcgg ataacgtgaa agaatggttt gtgcctcagg aatgcgtacc
    39541 tgatccagca tccgcttcgc ctcgaccatc gcttcttcac gactggcgtt ctgatgcaga
    39601 cgaattgatt cggcaatctg ttcacccaca gtaaataccg ggttcagcga tgtcatcggc
    39661 tcctgaaata tcatcgccat atccgcaccg cgaacatggc gcatttgtgc agcgctctgc
    39721 tcgctaagtt caatcacttc gcgactgcgc cgctgcaaca gcattttatc gcactgtact
    39781 aaaccgcccg cctgttccaa caggcgcatc aatgccaacg cagtcactga cttaccggag
    39841 ccggattcgc caacaattgc cagcgtctca ccgcgttgca gactaaaaga gagattgcgg
    39901 accgcagcta ttttctgctg gtcctgcata aaggcaatat tcagattttc aaccgccagc
    39961 acattaccgg catcaagttc atcactgtgt ggcaacggtg tccccttttt cacggtagat
    40021 accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga tacattcctt cggtgttaaa
    40081 cggtagcgcg acattccctt catggtcgat agcgattaag ccaccgctac cgccaagcgc
    40141 agggagtttt tccattacta cccgctcgca ggcttccgcg agacttaatc cgccgtaatc
    40201 cattaacgcg gcgatgtcat atgccgccag cgcgcggatg aagacttcgc ccgtgccggt
    40261 acaagaaacc gccacactgg cgttattggc gtagcatccg gcacccacta agggactatc
    40321 gccaactcgt ccgggtaatt tattggtcat tccgcctgtg gacgtggctg ccgccaaatt
    40381 gccgtctaaa tccaacgcca cggcccccac ggtgcccatt ttctgttttt catccagtgg
    40441 cgcaccgcta tggtcgagga cggttgcccc ttccttgcgc gctgccagta gttgttcata
    40501 acgcaaaggc gtggagaaaa tctccggcga gacgcgctcc atgccacgag caaacgcaaa
    40561 attttctgcc ccttcgccaa tcatcatcac atgcgggctt tgctccatca ccagccgggc
    40621 ggcaagaacc ggattacgca gatgactaac gcccgccacc gcaccggctt tcagggtgtt
    40681 accatccatc acacaggcgt ccagttcatg ggtttcatca cgcgtaaaga cagcgccaat
    40741 tccggcgtta aacagtggac actcttccag cagacgcacc gcttccgtca ccacatccag
    40801 cgcactttcg cccgcttcca gcattttctg cccggtttca acaatggcag acaacgcctc
    40861 aatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg ctaattgcac ctgcgccacc
    40921 atgaattgca atgactgctt tgcccatagt attcgtccat taagggtggc tcgccacttt
    40981 tgctataaat atcagaattt ttatgaatta ctcattcaat ttttgaatat agaaagatgg
    41041 atatggcatg taaaggcagg agccaacgct acgcccgagg tggcatacag tctgtggcgt
    41101 ttttctgaca taataggcaa attcgatttt gcctccgaag gagtgttttc atggaattta
    41161 ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat gctttctcgc gtcaccccac
    41221 tgaccgccca ggaaacgctg ccactggtac agtgttttgg tcgtattctg gcgagcgatg
    41281 tcgtttcgcc gcttgatgtt ccggggtttg ataactccgc aatggacggc tacgcggtgc
    41341 gtttagccga tattgcctcc aggcaaccgc tgcccgttgc cggtaaatcc tttgccggtc
    41401 agccgtacca tggtgaatgg cctgcgggta cctgcattcg tattatgacc ggtgcgccgg
    41461 tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac tgaacaaacg gacaatggcg
    41521 tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg ccgtcgcggt gaagatatct
    41581 ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac taccgctgaa ctgccagtga
    41641 ttgcttcact ggggattgcc gaagttccgg tgattcgtaa agtgcgtgta gcgctttttt
    41701 ctaccggtga tgaactccag ttgcccggtc agccactggg cgatggccaa atctacgata
    41761 ccaaccgtct cgccgtacac ctgatgttag aacagttggg atgcgaggta atcaacttag
    41821 ggattatccg cgacgatcca catgccctgc gcgccgcatt tattgaagcc gacagccagg
    41881 cggatgtggt gatcagttcc ggcggtgttt cagtgggtga agcggattac accaaaacca
    41941 ttcttgaaga gctgggggag atcgccttct ggaagctggc gattaaaccg ggtaaaccgt
    42001 tcgcgttcgg taaactttct aatagctggt tctgcggcct gccgggcaac ccggtttcag
    42061 cgacgctgac cttctatcaa ctggtacagc ctttgctggc aacactaagc ggcaacaccg
    42121 ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc ccgcctgaaa aaaacgccag
    42181 gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc cgacggtgaa cttgaagtga
    42241 cgaccaccgg acatcagggt tcacatatat ttagctcctt tagtctcggc aactgcttta
    42301 tcgtgctgga acgcgatcgc ggcaatgtgg acgtgggcga atgggtggaa gtagaaccgt
    42361 ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc gatcaggaga tgctgcgcta
    42421 caaccggcaa atcattctgc gcggttttga ttttgacggt caggaggcgc taaaagattc
    42481 tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca gcttcgcagt atctggcaag
    42541 cgccggtgtc ggcaacctga cgctgctcga cttcgacacg gtttcgctct cgaatctgca
    42601 acgccagaca ctgcacagtg atgccacggt cgggcaaccg aaggtggaat ccgcccgtta
    42661 cgccctgacg cggatcaacc cacatatcgc gattacgcca gtcaatgcac tgctagatga
    42721 cgcagaactt gcagcattga ttgctgaaca cgatctggtg ctcgactgta cggataacgt
    42781 tgcggtacgt aatcaactga acgcaggctg ttttgccgcg aaggtgccgc tggtttccgg
    42841 cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact tatcaggacg gcgaaccgtg
    42901 ctatcgctgc cttagccgtt tgtttggcga gaacgcatta acctgcgtag aagcaggcgt
    42961 gatggcaccg ttgatcggcg taattggttc gttgcaggca atggaagcga tcaaactgct
    43021 ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg tacgatgcga tgacctgtca
    43081 gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag gtgtgcgggc agtaattgcc
    43141 acctccaggc ctgataagac gcgacaagcg tcgcatcagg catttgctcc agacgccaga
    43201 tgcggcgtga acgccttatc tggcctacgg ttcgggcaca gatttccaag cgcggcaggc
    43261 atcggtgcac tccgccacgt catcagcaat ttatgaggat gcagaacgtc cggttaaatc
    43321 gacgttctgc caaacgctcc cagccagtcc tgttcaaact tcgccacagc ggcttccacc
    43381 gccggatagc taatcatctg ttgtgccaca tccagtggca gcgtaattga ttcacatcct
    43441 gccagtaagc agtccagcgc ctgacgcggg gttttgaaac tcgctgccag cactttcgcc
    43501 tgcggcgcat gcattttcaa taactggtgt aagtcggtca cagtctgaat gccgctaccg
    43561 ccctgagcat caatacgatt aacgtaaggc gcaacatatt ccgcacctgc cagcgccgac
    43621 agcagccctt gtgctgcgcc atataccgcg gttcccagcg ttggaatccc ttccgctttt
    43681 aacatcttaa tagctgccag cccctcggcg gtcaccggaa ctttcaccac gatatccgca
    43741 ataatagaac gcaatttacg tgcgtcattc accatccctt cagcagtggt agccattacc
    43801 tgggcaaaca gacgcccctg accgcccatc gcttcatgaa gttgcggaag cacaacatcc
    43861 agcggttttt tacccgcggc gataatgctt gggttagtgg tcacacccgc cagcggaaaa
    43921 atacgtgaca gcgccttcac cgcaacaacg tatgaagtat ccagatacag ttccataacc
    43981 atcctcaaaa tgtacttttt gtctgaatgt agactatcac gggaaaactc tctgaacagt
    44041 tgacgcacat caatctaact ttcattcgaa agtattttaa tgtttgaacg aaacagagag
    44101 gcaactatga ttttcaatat tcagcgctac tcgacccatg atggccccgg tatccgcacg
    44161 gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt gtcagaaccc ggaaagccgc
    44221 gcccgcacgc aggatctgct gtatgacgca cgactgtgtc tggaaggctg cgagctgtgc
    44281 gctaaggctg cgccggaagt gattgagcgc gcgctgaatg gtttgcttat tcatcgggaa
    44341 aagttaaccc cggagcatct gacggcgtta accgactgct gtccgacaca ggcattaacc
    44401 gtgtgtggtg aagtgaaaag cgttgaggag atcatgacga ccgttctgcg cgataaaccg
    44461 ttttacgatc gcagcggcgg cggtttaacg ctttcgggtg gtgagccctt tatgcagccg
    44521 gaaatggcga tggcgctact gcaagccagc cacgaggcag gcattcatac tgcggtagaa
    44581 acctgtctgc atgtgccgtg gaaatatatc gccccttctc tgccctatat cgatctgttt
    44641 cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac agtggaccga cggtaacgcc
    44701 gccagagtgc tggataacct gaaaaaactc gccgcagcgg gcaaaaaaat cattatccgc
    44761 gtgccgctga ttcagggctt taatgccgac gaaacctctg taaaagccat taccgatttt
    44821 gccgccgacg agctgcacgt tggcgaaatt cattttctgc cctaccacac gctgggcatc
    44881 aacaaatatc acttacttaa tctgccctat gacgccccgg aaaaaccgct tgatgcgcca
    44941 gaactgctcg actttgccca gcagtatgcc tgccagaaag ggttaaccgc gaccttacga
    45001 ggataacaac catgaccaca ctgaaactgg acacgctcag cgaccgcatt aaagcgcaca
    45061 aaaatgcgct ggtgcatatt gtgaaaccgc cagtctgtac cgagcgcgcg cagcactata
    45121 ccgagatgta tcaacaacat ctcgataagc cgatcccggt acgtcgcgcg ctggcactga
    45181 cgcatcacct ggcgaatcgc accatctgga tcaaacacga tgagttgatc attggcaacc
    45241 aggcaagcga agttcgcgcc gcgccgatct tcccggaata tactgtctcg tggatcgaaa
    45301 aagagattga tgatctggca gatcgtcccg gtgctggctt tgcggtgagc gaagagaaca
    45361 aacgcgttct gcatgaagtg tgcccgtggt ggcgcggtca gaccgtacag gatcgctgct
    45421 acggcatgtt taccgatgag caaaaaggtc tgctggcgac cggaatcatt aaagcggaag
    45481 gcaatatgac ctccggcgat gcgcacctgg cggtgaattt cccgctgctg ctggaaaaag
    45541 gtcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc gcgcatcaat ctgacggtgc
    45601 tggaagattt acatggcgaa caattcctga aagcgattga tatcgtgctg gtggcagtca
    45661 gtgaacacat tgaacgtttc gctgccctgg cgcgtgaaat ggctgctacc gaaactcgcg
    45721 aaagccgtcg cgatgaactg ctgacgatgg cagaaaactg cgatcttatc gcccaccagc
    45781 cgccgcagac tttctggcag gcgctgcaac tgtgttactt catccaattg attttgcaga
    45841 tcgaatctaa cggtcactcg gtatcgtttg gtcgtatgga ccagtatctc tacccgtact
    45901 atcgccgcga cgttgaactc aaccagacgc tggatcgcga acacgccatc gagatgctgc
    45961 atagctgctg gctgaaactg ctggaagtga acaagatccg ctccggctca cactcaaaag
    46021 cctctgcggg aagtccgctg tatcagaacg ttaccattgg cggacaaaat ctggttgatg
    46081 gtcaaccaat ggatgcagtg aatcccctct cttacgcgat cctcgaatcc tgcggtcgcc
    46141 tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc aggaatgagt aatgatttcc
    46201 tcgacgcctg cgtacaggtg atccgttgcg gcttcgggat gccggcgttc aacaacgacg
    46261 aaatcgtgat cccggaattt attaaactcg gtattgaacc gcaggacgct tacgactacg
    46321 cagcgattgg ttgtatcgaa accgccgtcg gtggcaaatg gggctatcgc tgtaccggca
    46381 tgagctttat caacttcgcc cgcgtgatgc tggcggcgct ggaaggcggg cgtgatgcca
    46441 ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc ggcaggcaac tttaacaatt
    46501 tcgatgaagt aatggacgcg tgggatacgc aaatccgtta ttacacgcgt aaatcaatcg
    46561 aaatcgaata tgtcgtcgac accatgctgg aagagaacgt acacgatatt ctctgctcgg
    46621 cgctggtgga tgactgtatt gagcgagcga aaagtatcaa gcaaggcggc gcgaaatatg
    46681 actgggtttc tggcctgcag gttggcattg ccaacctcgg caacagcctg gcggcagtga
    46741 agaaactggt atttgaacag ggcgcgattg gtcagcaaca gctggctgcc gcactggcgg
    46801 atgacttcga tggcctgact catgagcaac tgcgtcagcg gctgattaac ggtgcgccga
    46861 agtacggcaa cgacgatgat accgtcgata cgctgctggc tcgcgcttat cagacctata
    46921 tcgacgaact gaaacagtac cataacccgc gctacggtcg tggtccggtt ggcggcaact
    46981 attacgcggg tacctcgtct atctccgcta acgtaccgtt tggcgcgcaa actatggcaa
    47041 caccggacgg gcgtaaagcc cacaccccgc tggcagaagg cgcaagcccg gcttccggta
    47101 ctgatcatct tggccctact gcggtcattg gctcggtggg taaactgcct acggcagcga
    47161 ttctcggcgg cgtgttgctc aaccagaaac tgaatccagc aacactggag aacgaatctg
    47221 acaagcagaa actgatgatc ctgctgcgca ccttctttga agtgcataaa ggctggcata
    47281 ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc gaaaaaacat ccggatcagt
    47341 accgcgatct ggtagtgcgt gtcgcgggct attccgcctt cttcaccgcg ctctctccag
    47401 acgcacagga cgatatcatc gcccgtactg aacatatgct gtaatctcct cggcccggcg
    47461 gcgtcgtcgc cgggctaaat cattcacatc atcaatttca tccttacttt cattcgaaat
    47521 ataatttgtg ctctgcgtca cattgttatt acactgttac ggtttcattt tcgagccagg
    47581 agcacaccta tgagcgtaaa agttatcgtc acagacatgg acggtacttt tcttaacgac
    47641 gccaaaacgt acaaccaacc acattttatg gcgcaatatc aggaactgaa aaagcgcggc
    47701 attaagttcg ttgtcgccag cggtaatcag tattaccagc ttatttcgtt ctttcctgag
    47761 ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac tggtttacga acatggcaag
    47821 caactgttcc acggcgaact gacccgacat gaatcgcgga ttgttattgg cgagttgcta
    47881 aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa gtgcatatgt cagcgaaaat
    47941 gcccccgaag catttgtcgc actgatggca aaacactacc atcgcctgaa agctgtaaaa
    48001 gattatcagg agattgacga cgtactgttc aagttttcgc tcaacctgcc agatgaacaa
    48061 atcccgttag tgatcgacaa actgcacata gcgctcgatg gcattatgaa acccgtcacc
    48121 agtggttttg gctttatcga cctgattatt cccggtctac ataaagcaaa cggtatttcg
    48181 cggttactga aacgctggga tctgtcaccg caaaatgtgg tagcgattgg cgacagcggt
    48241 aacgatgcgg agatgctgaa aatggcgcgt tattcctttg cgatgggcaa tgctgcggaa
    48301 aacattaaac aaatcgcccg ttacgctacc gatgataata atcatgaagg cgcgctgaat
    48361 gtgattcagg cggtgctgga taacacatcc ccttttaaca gctgacctct ctaccggagc
    48421 cgtgcgctcc ggttttccct tctttttaca tcctgtacat tacatcaaat cattaaactt
    48481 gcattaactt atttataact tatattgtca catgcaaatc agaatggttt cgaatgaaag
    48541 ttaatgagag gtggttatgg cttctacttt taccagcgac acattgcctg ccgatcacaa
    48601 agcagctatc cgtcagatga agcacgcgct gcgggcgcag cttggcgacg tccagcagat
    48661 ctttaatcag ctaagcgatg acattgccac gcgagtggct gaaatcaacg cactcaaagc
    48721 acagggcgat gccgtctggc cggtgctgtc ttatgccgat atcaaagcag gtcatgttac
    48781 tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg gtgataaaag gccatttccc
    48841 ccgcgaacaa gcgctaggct gggatcagtc gatgctggac tatctggacc gcaaccgctt
    48901 tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg acgctcagcg cttcacgtcc
    48961 cgagatttac cccatctact ggtcgcaggc gcaaatgcag gcccgccaga gtgaagaaat
    49021 ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt gaaagtgatg gaaagcaatg
    49081 gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc cgccgccgtc cgcccggaac
    49141 gacctccaaa ggtcttggag cgcataccga ctccggggcg ctggaacgct ggctgcttcc
    49201 agcgtatcag cgcgttttcg ccaacgtctt taatggcaat ctggcgcaat atgatccctg
    49261 gcatgcggca catcgtacgg aagttgaaga gtacacggtg gacaacacca ccaaatgttc
    49321 cgtgtttcgg acattccagg gctggacagc gctctctgat atgctgcctg gtcaggggtt
    49381 gctgcacgtt gtgcccattc ctgaagccat ggcgtacgta ctgttacgtc cgctgcttga
    49441 tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga agagtgttgc cggtatcaga
    49501 gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt ccaaaactcg aggccggaga
    49561 ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc cccgttgaaa atcaacaggg
    49621 ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc gagaaaaatc ttgcctacgc
    49681 gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg ggcgacttcc cgcgcgagga
    49741 ctatgaaaca aactgggaag gacactttac gcttgccgac ctcaacattc acggtaagcg
    49801 agcgttgggc atggatgttt gagagttaat ggaagtgaat tgatgtggga ttaagaaaat
    49861 cgattcgacg ttactcagat tgctgacaag tgcgctttct taatgcctga tgcgctacgc
    49921 ttatc
//
LOCUS       DAZGAN020000025        48947 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGAN020000025 DAZGAN020000000
VERSION     DAZGAN020000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 48947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.025"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            389..1606
                     /gene="alaA"
                     /locus_tag="ACHKYO_RS08190"
                     /old_locus_tag="pgaptmp_001638"
     CDS             389..1606
                     /gene="alaA"
                     /locus_tag="ACHKYO_RS08190"
                     /old_locus_tag="pgaptmp_001638"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HGU4428502.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1690..2289
                     /gene="yfbR"
                     /locus_tag="ACHKYO_RS08195"
                     /old_locus_tag="pgaptmp_001639"
     CDS             1690..2289
                     /gene="yfbR"
                     /locus_tag="ACHKYO_RS08195"
                     /old_locus_tag="pgaptmp_001639"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4428503.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2348..4180)
                     /locus_tag="ACHKYO_RS08200"
                     /old_locus_tag="pgaptmp_001640"
     CDS             complement(2348..4180)
                     /locus_tag="ACHKYO_RS08200"
                     /old_locus_tag="pgaptmp_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGU4428504.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLVDEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4267..4917)
                     /gene="hxpA"
                     /locus_tag="ACHKYO_RS08205"
                     /old_locus_tag="pgaptmp_001641"
     CDS             complement(4267..4917)
                     /gene="hxpA"
                     /locus_tag="ACHKYO_RS08205"
                     /old_locus_tag="pgaptmp_001641"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HGU4428505.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4928..5422)
                     /locus_tag="ACHKYO_RS08210"
                     /old_locus_tag="pgaptmp_001642"
     CDS             complement(4928..5422)
                     /locus_tag="ACHKYO_RS08210"
                     /old_locus_tag="pgaptmp_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HGU4428506.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5505..5960)
                     /gene="yfbV"
                     /locus_tag="ACHKYO_RS08215"
                     /old_locus_tag="pgaptmp_001643"
     CDS             complement(5505..5960)
                     /gene="yfbV"
                     /locus_tag="ACHKYO_RS08215"
                     /old_locus_tag="pgaptmp_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HGU4428507.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6298..7500
                     /gene="ackA"
                     /locus_tag="ACHKYO_RS08220"
                     /old_locus_tag="pgaptmp_001644"
     CDS             6298..7500
                     /gene="ackA"
                     /locus_tag="ACHKYO_RS08220"
                     /old_locus_tag="pgaptmp_001644"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGU4428508.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7575..9719
                     /gene="pta"
                     /locus_tag="ACHKYO_RS08225"
                     /old_locus_tag="pgaptmp_001645"
     CDS             7575..9719
                     /gene="pta"
                     /locus_tag="ACHKYO_RS08225"
                     /old_locus_tag="pgaptmp_001645"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU4428509.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9909..11429
                     /gene="yfcC"
                     /locus_tag="ACHKYO_RS08230"
                     /old_locus_tag="pgaptmp_001646"
     CDS             9909..11429
                     /gene="yfcC"
                     /locus_tag="ACHKYO_RS08230"
                     /old_locus_tag="pgaptmp_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HGU4428510.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSELRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11462..12004)
                     /gene="yfcD"
                     /locus_tag="ACHKYO_RS08235"
                     /old_locus_tag="pgaptmp_001647"
     CDS             complement(11462..12004)
                     /gene="yfcD"
                     /locus_tag="ACHKYO_RS08235"
                     /old_locus_tag="pgaptmp_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HGU4428511.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12062..12613)
                     /gene="yfcE"
                     /locus_tag="ACHKYO_RS08240"
                     /old_locus_tag="pgaptmp_001648"
     CDS             complement(12062..12613)
                     /gene="yfcE"
                     /locus_tag="ACHKYO_RS08240"
                     /old_locus_tag="pgaptmp_001648"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HGU4428512.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12669..13313)
                     /gene="yfcF"
                     /locus_tag="ACHKYO_RS08245"
                     /old_locus_tag="pgaptmp_001649"
     CDS             complement(12669..13313)
                     /gene="yfcF"
                     /locus_tag="ACHKYO_RS08245"
                     /old_locus_tag="pgaptmp_001649"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HGU4428513.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13449..14096
                     /gene="yfcG"
                     /locus_tag="ACHKYO_RS08250"
                     /old_locus_tag="pgaptmp_001650"
     CDS             13449..14096
                     /gene="yfcG"
                     /locus_tag="ACHKYO_RS08250"
                     /old_locus_tag="pgaptmp_001650"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HGU4428514.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14153..14515
                     /gene="folX"
                     /locus_tag="ACHKYO_RS08255"
                     /old_locus_tag="pgaptmp_001651"
     CDS             14153..14515
                     /gene="folX"
                     /locus_tag="ACHKYO_RS08255"
                     /old_locus_tag="pgaptmp_001651"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HGU4428515.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14536..15429
                     /locus_tag="ACHKYO_RS08260"
                     /old_locus_tag="pgaptmp_001652"
     CDS             14536..15429
                     /locus_tag="ACHKYO_RS08260"
                     /old_locus_tag="pgaptmp_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HGU4428516.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15477..16367)
                     /gene="rpnB"
                     /locus_tag="ACHKYO_RS08265"
                     /old_locus_tag="pgaptmp_001653"
     CDS             complement(15477..16367)
                     /gene="rpnB"
                     /locus_tag="ACHKYO_RS08265"
                     /old_locus_tag="pgaptmp_001653"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HGU4428517.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVLMVTRLSPDDLIAQSH"
     gene            complement(16564..17337)
                     /gene="hisP"
                     /locus_tag="ACHKYO_RS08270"
                     /old_locus_tag="pgaptmp_001654"
     CDS             complement(16564..17337)
                     /gene="hisP"
                     /locus_tag="ACHKYO_RS08270"
                     /old_locus_tag="pgaptmp_001654"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HGU4428518.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17345..18061)
                     /locus_tag="ACHKYO_RS08275"
                     /old_locus_tag="pgaptmp_001655"
     CDS             complement(17345..18061)
                     /locus_tag="ACHKYO_RS08275"
                     /old_locus_tag="pgaptmp_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4428519.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18058..18744)
                     /gene="hisQ"
                     /locus_tag="ACHKYO_RS08280"
                     /old_locus_tag="pgaptmp_001656"
     CDS             complement(18058..18744)
                     /gene="hisQ"
                     /locus_tag="ACHKYO_RS08280"
                     /old_locus_tag="pgaptmp_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HGU4428520.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18834..19616)
                     /gene="hisJ"
                     /locus_tag="ACHKYO_RS08285"
                     /old_locus_tag="pgaptmp_001657"
     CDS             complement(18834..19616)
                     /gene="hisJ"
                     /locus_tag="ACHKYO_RS08285"
                     /old_locus_tag="pgaptmp_001657"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HGU4428521.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19837..20619)
                     /gene="argT"
                     /locus_tag="ACHKYO_RS08290"
                     /old_locus_tag="pgaptmp_001658"
     CDS             complement(19837..20619)
                     /gene="argT"
                     /locus_tag="ACHKYO_RS08290"
                     /old_locus_tag="pgaptmp_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HGU4428522.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20885..21454)
                     /gene="ubiX"
                     /locus_tag="ACHKYO_RS08295"
                     /old_locus_tag="pgaptmp_001659"
     CDS             complement(20885..21454)
                     /gene="ubiX"
                     /locus_tag="ACHKYO_RS08295"
                     /old_locus_tag="pgaptmp_001659"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HGU4428523.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21549..23066)
                     /gene="purF"
                     /locus_tag="ACHKYO_RS08300"
                     /old_locus_tag="pgaptmp_001660"
     CDS             complement(21549..23066)
                     /gene="purF"
                     /locus_tag="ACHKYO_RS08300"
                     /old_locus_tag="pgaptmp_001660"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGU4428524.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23103..23591)
                     /gene="cvpA"
                     /locus_tag="ACHKYO_RS08305"
                     /old_locus_tag="pgaptmp_001661"
     CDS             complement(23103..23591)
                     /gene="cvpA"
                     /locus_tag="ACHKYO_RS08305"
                     /old_locus_tag="pgaptmp_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HGU4428525.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23850..24512)
                     /gene="dedD"
                     /locus_tag="ACHKYO_RS08310"
                     /old_locus_tag="pgaptmp_001662"
     CDS             complement(23850..24512)
                     /gene="dedD"
                     /locus_tag="ACHKYO_RS08310"
                     /old_locus_tag="pgaptmp_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HGU4428526.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24502..25770)
                     /gene="folC"
                     /locus_tag="ACHKYO_RS08315"
                     /old_locus_tag="pgaptmp_001663"
     CDS             complement(24502..25770)
                     /gene="folC"
                     /locus_tag="ACHKYO_RS08315"
                     /old_locus_tag="pgaptmp_001663"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HGU4428527.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25840..26754)
                     /gene="accD"
                     /locus_tag="ACHKYO_RS08320"
                     /old_locus_tag="pgaptmp_001664"
     CDS             complement(25840..26754)
                     /gene="accD"
                     /locus_tag="ACHKYO_RS08320"
                     /old_locus_tag="pgaptmp_001664"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGU4428528.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26910..27569)
                     /locus_tag="ACHKYO_RS08325"
                     /old_locus_tag="pgaptmp_001665"
     CDS             complement(26910..27569)
                     /locus_tag="ACHKYO_RS08325"
                     /old_locus_tag="pgaptmp_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4428529.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27652..28464)
                     /gene="truA"
                     /locus_tag="ACHKYO_RS08330"
                     /old_locus_tag="pgaptmp_001666"
     CDS             complement(27652..28464)
                     /gene="truA"
                     /locus_tag="ACHKYO_RS08330"
                     /old_locus_tag="pgaptmp_001666"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGU4428530.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28464..29477)
                     /locus_tag="ACHKYO_RS08335"
                     /old_locus_tag="pgaptmp_001667"
     CDS             complement(28464..29477)
                     /locus_tag="ACHKYO_RS08335"
                     /old_locus_tag="pgaptmp_001667"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU4428531.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29543..30679)
                     /gene="pdxB"
                     /locus_tag="ACHKYO_RS08340"
                     /old_locus_tag="pgaptmp_001668"
     CDS             complement(29543..30679)
                     /gene="pdxB"
                     /locus_tag="ACHKYO_RS08340"
                     /old_locus_tag="pgaptmp_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HGU4428532.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGYVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30778..31773
                     /gene="flk"
                     /locus_tag="ACHKYO_RS08345"
                     /old_locus_tag="pgaptmp_001669"
     CDS             30778..31773
                     /gene="flk"
                     /locus_tag="ACHKYO_RS08345"
                     /old_locus_tag="pgaptmp_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HGU4428533.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRVMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31770..32948)
                     /locus_tag="ACHKYO_RS08350"
                     /old_locus_tag="pgaptmp_001670"
     CDS             complement(31770..32948)
                     /locus_tag="ACHKYO_RS08350"
                     /old_locus_tag="pgaptmp_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4428534.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPVLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33213..34433)
                     /gene="fabB"
                     /locus_tag="ACHKYO_RS08355"
                     /old_locus_tag="pgaptmp_001671"
     CDS             complement(33213..34433)
                     /gene="fabB"
                     /locus_tag="ACHKYO_RS08355"
                     /old_locus_tag="pgaptmp_001671"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HGU4428535.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34592..36598
                     /gene="mnmC"
                     /locus_tag="ACHKYO_RS08360"
                     /old_locus_tag="pgaptmp_001672"
     CDS             34592..36598
                     /gene="mnmC"
                     /locus_tag="ACHKYO_RS08360"
                     /old_locus_tag="pgaptmp_001672"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HGU4428536.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDAGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36719..36997)
                     /locus_tag="ACHKYO_RS08365"
                     /old_locus_tag="pgaptmp_001673"
     CDS             complement(36719..36997)
                     /locus_tag="ACHKYO_RS08365"
                     /old_locus_tag="pgaptmp_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HGU4428537.1"
                     /translation="MIAEFESRILALIDDMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37031..37579)
                     /gene="epmC"
                     /locus_tag="ACHKYO_RS08370"
                     /old_locus_tag="pgaptmp_001674"
     CDS             complement(37031..37579)
                     /gene="epmC"
                     /locus_tag="ACHKYO_RS08370"
                     /old_locus_tag="pgaptmp_001674"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HGU4428538.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37579..38388)
                     /locus_tag="ACHKYO_RS08375"
                     /old_locus_tag="pgaptmp_001675"
     CDS             complement(37579..38388)
                     /locus_tag="ACHKYO_RS08375"
                     /old_locus_tag="pgaptmp_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGU4428539.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(38388..39212)
                     /gene="mepA"
                     /locus_tag="ACHKYO_RS08380"
                     /old_locus_tag="pgaptmp_001676"
     CDS             complement(38388..39212)
                     /gene="mepA"
                     /locus_tag="ACHKYO_RS08380"
                     /old_locus_tag="pgaptmp_001676"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HGU4428540.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39216..40301)
                     /gene="aroC"
                     /locus_tag="ACHKYO_RS08385"
                     /old_locus_tag="pgaptmp_001677"
     CDS             complement(39216..40301)
                     /gene="aroC"
                     /locus_tag="ACHKYO_RS08385"
                     /old_locus_tag="pgaptmp_001677"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGU4428541.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40336..41268)
                     /gene="prmB"
                     /locus_tag="ACHKYO_RS08390"
                     /old_locus_tag="pgaptmp_001678"
     CDS             complement(40336..41268)
                     /gene="prmB"
                     /locus_tag="ACHKYO_RS08390"
                     /old_locus_tag="pgaptmp_001678"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU4428542.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41434..41985
                     /gene="smrB"
                     /locus_tag="ACHKYO_RS08395"
                     /old_locus_tag="pgaptmp_001679"
     CDS             41434..41985
                     /gene="smrB"
                     /locus_tag="ACHKYO_RS08395"
                     /old_locus_tag="pgaptmp_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HGU4428543.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42058..42912)
                     /locus_tag="ACHKYO_RS08400"
                     /old_locus_tag="pgaptmp_001680"
     CDS             complement(42058..42912)
                     /locus_tag="ACHKYO_RS08400"
                     /old_locus_tag="pgaptmp_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HGU4428544.1"
                     /translation="MKILRHVLCLLLLLMFNTCGQAATKVGELYVPAGASSPATEFEF
                     HLIIATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSRVSMSACPGLEQ
                     YDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDDDFPDYIGPTKV
                     SSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCNGNNFDE
                     RGAYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCR
                     FTYILNMY"
     gene            complement(42914..43453)
                     /locus_tag="ACHKYO_RS08405"
                     /old_locus_tag="pgaptmp_001681"
     CDS             complement(42914..43453)
                     /locus_tag="ACHKYO_RS08405"
                     /old_locus_tag="pgaptmp_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4428545.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGAQAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVSGQSAHLPVVFKLKDCKGPAGYNVKVTLSGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(43450..43938)
                     /locus_tag="ACHKYO_RS08410"
                     /old_locus_tag="pgaptmp_001682"
     CDS             complement(43450..43938)
                     /locus_tag="ACHKYO_RS08410"
                     /old_locus_tag="pgaptmp_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4428546.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            complement(43935..44444)
                     /locus_tag="ACHKYO_RS08415"
                     /old_locus_tag="pgaptmp_001683"
     CDS             complement(43935..44444)
                     /locus_tag="ACHKYO_RS08415"
                     /old_locus_tag="pgaptmp_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4428547.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(44460..45212)
                     /locus_tag="ACHKYO_RS08420"
                     /old_locus_tag="pgaptmp_001684"
     CDS             complement(44460..45212)
                     /locus_tag="ACHKYO_RS08420"
                     /old_locus_tag="pgaptmp_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4428548.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(45232..47877)
                     /gene="yfcU"
                     /locus_tag="ACHKYO_RS08425"
                     /old_locus_tag="pgaptmp_001685"
     CDS             complement(45232..47877)
                     /gene="yfcU"
                     /locus_tag="ACHKYO_RS08425"
                     /old_locus_tag="pgaptmp_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HGU4428549.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(47959..48522)
                     /locus_tag="ACHKYO_RS08430"
                     /old_locus_tag="pgaptmp_001686"
     CDS             complement(47959..48522)
                     /locus_tag="ACHKYO_RS08430"
                     /old_locus_tag="pgaptmp_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4428550.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        12036 a        13195 c        12184 g        11532 t
ORIGIN      
        1 ttaattccgc ttaacaatag tttcacacct tgcccccatt acgacacctc ggaaatcatc
       61 acgctgttaa actctccgta atgcggaaat atcatcatcg cgtttcatta gtgaattctt
      121 aaatgaggca ttttcacaca attatcttac agataaaaaa accagactta caattaagaa
      181 tcagaacaat tcactatata acattgcatg taaagcatat acacctcatt attttgtcat
      241 tattaagtta ttaacagcac aatcgagcct tcccctctgg caaaatctta ttctgcagac
      301 cttcaaaaca ccgtcctggg ggagtacatt gttctaagct gacttccacg gcagggagtg
      361 gcgataacag caaaaaaggt caagattcat gtcccccatt gaaaaatcca gcaaattaga
      421 gaatgtctgt tatgacatcc gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga
      481 aggtaacaag gtactgaaac tgaacatcgg caacccagcc ccgttcggtt ttgacgcgcc
      541 agatgaaatc ctcgttgacg tgatacgcaa cctgcctacc gctcaagggt attgcgattc
      601 caaaggtctt tactccgcgc gtaaagccat catgcagcac taccaggctc gtggcatgcg
      661 tgatgttacc gtggaagata tttacatcgg caatggtgta tcggagctta tcgttcaggc
      721 aatgcaggca ttgctgaaca gcggggacga aatgttggtt cctgcaccag attacccact
      781 atggaccgcg gcggtttcgc tttccagcgg taaagcggtg cattatcttt gcgatgaatc
      841 ctctgactgg ttcccggacc tcgatgatat tcgcgctaaa attacgcctc gtacgcgtgg
      901 gatcgttata atcaacccaa ataacccaac cggcgcggta tattccaaag agcttttaat
      961 ggagattgtg gagattgcac gtcagcataa tctcattatc ttcgccgatg aaatttatga
     1021 caaaattctt tacgacgacg ctgagcatca ctcaattgcg ccgctggcac ctgacctgct
     1081 gaccattacc tttaacggac tgtcgaaaac gtaccgcgtt gcaggcttcc gtcaggggtg
     1141 gatggtgttg aacgggccga aaaaacacgc caaaggctac atcgaaggtc tggaaatgct
     1201 ggcttcaatg cgcctgtgtg ctaacgttcc tgcgcaacac gccattcaga ccgcgctggg
     1261 tggttatcag agcatcagtg aatttattac ccctggcggt cgtctttatg agcagcgtaa
     1321 ccgcgcgtgg gaactgatca acgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc
     1381 gctgtatatg ttcccgaaaa tcgacgccaa acgctttaac attcacgacg atcagaaaat
     1441 ggtgctggat ttcctgttgc aggaaaaagt tctgttggtg caagggacgg cattcaactg
     1501 gccgtggccg gatcacttcc gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc
     1561 tttgagcaag ttcgcgcgtt tcctttctgg ttatcatcag ctgtaatctt aatttcactg
     1621 ccggagattg catccggcag cgttatcccg ccacaatgac ctgatgatgt catcatacgt
     1681 aaggtcacta tgaaacagag ccattttttt gcccatctct cccgcctgaa actcattaac
     1741 cgctggccgc tcatgcgcaa cgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta
     1801 gcgatggtcg cccatgcgct ggcagctatc aaaaatcgga aatttggcgg taatgtcaac
     1861 gccgaacgta tcgctttact ggcgatgtac cacgatgcct cagaagtgct caccggcgat
     1921 ctccctactc cggtgaaata cttcaattcg caaatcgctc aggagtacaa ggctattgaa
     1981 aaaatcgctc agcaaaaact ggtcgatatg gttccggaag agctgcggga tatctttgcg
     2041 ccgttaattg acgagcatgc atatagcgat gaagaaaaat cgctggtgaa acaggcagat
     2101 gcactgtgtg catatctgaa atgtctggaa gaactcgcgg ccggaaataa tgaattcttg
     2161 ctggcaaaaa cgcgactgga agcgacgctt gaagcgcgtc gcagccagga gatggactac
     2221 ttcatggaag tatttgttcc cagcttccat ctttcgctcg atgagattag ccaggattca
     2281 ccgctgtaag cagccggagt ctgcgtcgca tcaggcaata agcgccggat gcgacatcag
     2341 gctcttgtca aaacggaaac agcatcggga tcatcaccac acaaaccgcc atcacgataa
     2401 tggtgaacgg tacccccaac ttcacaaagt cactgaagct gtaatttccc ggacctaaaa
     2461 ccagtgtgtt aacaggtgaa gaaaccggtg tcataaaggc ggcggatgct gccatcgcca
     2521 cgaccatcgc gaatggataa ggcgacaccc ccatcgtttt ggcagcagcc agcgcaatcg
     2581 gagccatcaa caccgccgtc gcggtattag agataaatag cccaataacc gccgacaaga
     2641 caaacaaaca ccccagcatc atatgtggcc cgtaaccacc gccaatgtcc atcagccctt
     2701 tcaccgccag cgcgacacct cccgttttct gtaatgccac agcaaacggc atcatcccaa
     2761 cgatcaaaat aatgctcggc cagtgaatgg atttataggc gctttcagca tctatacagc
     2821 ggaatttccc catcagcagg caggcgatga tagcggcaac aggattagga atttcatctg
     2881 tcagcattaa cgccaccatt agcaccagac agaaaatggc atggggtgcc tggctgtgcg
     2941 cgggcgatgc ttcactcacc tcttccggta agttcagcgc tacgaagtcg cggccctgtt
     3001 tggccagcat accgatcagt ttccagttac ccacaaccag gatgatatcg cccagcagca
     3061 gaggctcatc caccagcgaa ccttccagcg ccacgccatt gcgctttagc cccaccacat
     3121 tcagtccgta gcgggtacga aaaccaattt cgcgcaccga tttaccaatc agttctgact
     3181 caggaattaa tgaaatctct gccatgccca catcaagggc ctggtcagaa aaatactcgc
     3241 cgcgcagtac catcggctcc agcaattgct cactacaaaa ttgccggaga tcgacatcag
     3301 ccgcagacat atcaataagc aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt
     3361 tcacgataac gcgacgaaaa cgccgccagc gttcaacacc gatgacgtta gcgccataac
     3421 gctcacgtaa tttgagatca tccagccgtt gacctatcat tggcgatccg gggcgaatag
     3481 ccagacgtcg cgcacgcccg gtcagtcgat attcacggat aagatcgcga aaggttcgac
     3541 gcgtccagcc ttcgcgctgc ggggtctggg tatccccttt cagcatgaaa cgcatcacta
     3601 acatatacaa aatacccagc accagcacga ccaggccaat aggtgttacg ctaaagaaac
     3661 tgaagccgtg atagccttca cgcagcaatt cactgttgac taccaggttc ggcggcgtcg
     3721 ccaccagcgt catcatgccg ctaatcagcc cggcaaaact taacggcatc atcagacgtg
     3781 acggcgaagt ttgcatacgc atggcaacgc ttaacaccac gggaataaag atagcgacaa
     3841 cgccggttga actcataaac gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca
     3901 ttttgatttc actgttgccc gccactttga ccagccatgt tcccattacg gtggcaacac
     3961 cggtacggac caaaccatcg ccaataataa acaaggcggc aatcaggaca acgttaggat
     4021 cagaaaagcc ggaaaatact tcggggaccg tcagcgttcc gcttaatgca aacgcgacaa
     4081 taacaaacaa agcgaccgca tccatacgca ctctgcccgt cgcaaacaag acgatggcaa
     4141 cggccagtaa tgaaagaacc caaatcaatt caccgttcac aacttatcct tgttaattga
     4201 gggggatgac ttgattctgc cataaaaaag ccccgacgag acggggctaa atcatgatca
     4261 ggtgtttcac tgaataataa catcgccatt tggctgtttg gtcacagtaa tttgctccag
     4321 actgtggagg accaaatcga cctcattcag gcgcggggta tctgccggag cgttaaccgc
     4381 aatgacatgg caacccgccg ccaggccaga aagcacgcca gcgggagcat cttccaccac
     4441 cacacactcc tgcggcgcaa gccccagcag ctgcgcacct aacagatacg catcaggttc
     4501 tggttttccg cgcttcactc gctcagcggt tacaaacacc tctggtgcgg gaagcccagc
     4561 tattttatgg cgcgctcgcg ctaccggcat ggagccagaa gtcacaatgg cccacggaat
     4621 acctgcttta ttcaaatgat tgagtaaggc gattgccccc ggaagcgcgg taataccttc
     4681 ggtttccgtg gcctcgatgt gctccagacg cgtaaactcg gcggcaatat cagcctcgga
     4741 tttgcccgcc ataaaatggc gcaaagaggt gatcgcctgt ttaccgtgaa tgaaagccag
     4801 cacctcttcc ggcgctaacc catgacgtct ggcccagttg ctccacgccc gttctaccgc
     4861 aggcagggaa tccaccagcg ttccatcaag atcaaacaga aaacctttgc accgcacgcg
     4921 ggcctcctca ggcgttgata atttgattaa tttcgttggc gctcaaatgg tactgacgcg
     4981 ggcaggcatg ccacacatta agcatgcgct gatatttttc ccacattggc gtctgggcgt
     5041 taaaaccgtg agttccggcg tcaaaatggg tatagcgccc ttccacatta accataaagc
     5101 ggacataacc gaggtaacgt gcttcagtgg cggcgtcaaa gccgaggaag gtgacacgac
     5161 gttcatcgat ggattgctgg tcctgcaaat tagaccagga aacatgcaac gcatgataca
     5221 tctccataat gtcgatgata gtgcggcagg tttcttcttt cagctcgcca aactcgcgat
     5281 ccaattcacg catctgtaat ccgtaaccac gctcaataat tgtttgcagg cgacggtaac
     5341 gttcagcatt tgccggatcg agcatagtca tcatcttgta ctgattagac aaaataagac
     5401 gttgcgcgtt ggtcatttcc attgttgact cctgtatcac tctactacgg tgaaaaaaaa
     5461 gaaggctgag tatgccttct tttatatgcg taatcagggg tcaattacaa atcatcaagg
     5521 aaagttttat ccagttgttt gaaggcgcgc ttaagcgtgt cagctaatgc ctggtaatca
     5581 ggcttgcctt caacgggtgc caacacctgt ccagactcct gcaatttacc gcgaacttca
     5641 taaaaccagt tgaggatagc agggggtaat ggcgttacag aacgcttgcc cagccaccat
     5701 aatccctgca tgggtaaact taaggcgaac agcgcagtgg caactgccgg cccaagctga
     5761 ccgcccaggg caatctgcca gcagagagta aatacggcga tcggcggcat aaaacggatc
     5821 gcataacgcg tcatcttgat aacgcgattt tcgacaaaga ccggggcaag gcgtttttcc
     5881 agcggccacg tctttgagta atgctgtccc cggcgaaaca agctaaaaaa attaacagaa
     5941 cgattatccg gcgttgacat gcttcacctc aacttcacat ataaagattc aaaaatttgt
     6001 gcaaattcac aactcagcgg gacaacgttc aaaacatttt gtcttccata cccactatca
     6061 ggtatccttt agcagcctga aggcctaagt agtacatatt cattgagtcg tcaaattcat
     6121 atacattatg ccattggctg aaaattacgc aaaatggcat agactcaaga tatttcttcc
     6181 atcatgcaaa aaaaatttgc agtgcatgat gttaatcata aatgtcggtg tcatcatgcg
     6241 ctacgctcta tggctccctg acgttttttt agccacgtat caattatagg tacttccatg
     6301 tcgagtaagt tagtactggt tctgaactgc ggtagttctt cactgaaatt tgccatcatc
     6361 gatgcagtaa atggtgaaga gtacctttct ggtttagccg aatgtttcca cctgcctgaa
     6421 gcacgtatca aatggaaaat ggacggcaat aaacaggaag cggctttagg tgcaggcgcc
     6481 gctcacagcg aagcgctcaa ctttatcgtt aatactattc tggcacaaaa accagaactg
     6541 tctgcgcagc tgactgctat cggtcaccgt atcgtacacg gcggcgaaaa gtataccagc
     6601 tccgtagtga tcgatgagtc tgttattcag ggtatcaaag atgcagcttc ttttgcaccg
     6661 ctgcacaacc cggctcacct gatcggtatc gaagaagctc tgaaatcttt cccacagctg
     6721 aaagacaaaa acgttgctgt atttgacacc gcgttccacc agactatgcc ggaagagtct
     6781 tacctctacg ccctgcctta caacctgtac aaagagcacg gcatccgtcg ttacggcgcg
     6841 cacggcacca gccacttcta tgtaacccag gaagcggcaa aaatgctgaa caaaccggta
     6901 gaagaactga acatcatcac ctgccacctg ggcaacggtg gttccgtttc tgctatccgc
     6961 aacggtaaat gcgttgacac ctctatgggc ctgaccccgc tggaaggtct ggtcatgggt
     7021 acccgttctg gtgatatcga tccggcgatc atcttccacc tgcacgacac cctgggcatg
     7081 agcgttgacg caatcaacaa actgctgacc aaagagtctg gcctgctggg tctgaccgaa
     7141 gtgaccagcg actgccgcta tgttgaagac aactacgcga cgaaagaaga cgcgaagcgc
     7201 gcaatggacg tttactgcca ccgcctggcg aaatacatcg gtgcctacac tgcgctgatg
     7261 gatggtcgtc tggacgctgt tgtattcact ggtggtatcg gtgaaaatgc cgcgatggtt
     7321 cgtgaactgt ctctgggcaa actgggcgtg ctgggctttg aagttgatca tgaacgcaac
     7381 ctggctgcac gtttcggcaa atctggtttc atcaacaaag aaggtacccg tcctgcggtg
     7441 gttatcccaa ccaacgaaga actggttatc gcgcaagacg cgagccgcct gactgcctga
     7501 tttcacaccg ccagctcagc tggcggtgct gttttgtaac ccgccaaatc ggcggtaacg
     7561 aaagaggata aaccgtgtcc cgtattatta tgctgatccc taccggaacc agcgtcggtc
     7621 tgaccagcgt cagccttggc gtgatccgtg caatggaacg caaaggcgtt cgtctgagcg
     7681 ttttcaaacc tatcgctcag ccgcgtaccg gtggcgatgc gcccgatcag actacgacta
     7741 tcgtgcgtgc gaactcttcc accacgacgg ccgctgaacc gctgaaaatg agctacgttg
     7801 aaggtctgct ttccagcaat cagaaagatg tgctgatgga agagatcgtc gcaaactacc
     7861 acgctaacac caaagacgct gaagtcgttc tggttgaagg tctggtcccg acacgtaagc
     7921 accagtttgc ccagtctctg aactacgaaa tcgctaaaac gctgaatgcg gaaatcgtct
     7981 tcgttatgtc tcagggcact gacaccccgg aacagctgaa agagcgtatc gaactgaccc
     8041 gcaacagctt cggcggtgcc aaaaacacca acatcaccgg cgttatcgtt aacaaactga
     8101 acgcaccggt tgatgaacag ggtcgtactc gcccggatct gtccgagatt ttcgacgact
     8161 cttccaaagc taaagtaaac aatgttgatc cggcgaagct gcaagaatcc agcccgctgc
     8221 cggttctcgg cgctgtgccg tggagctttg acctgatcgc gactcgtgcg atcgatatgg
     8281 ctcgccacct gaatgcgacc atcatcaacg aaggcgacat caatactcgc cgcgttaaat
     8341 ccgtcacttt ctgcgcacgc agcattccgc acatgctgga gcacttccgt gccggttctc
     8401 tgctggtgac ttccgcagac cgtcctgacg tgctggtggc cgcttgcctg gcagccatga
     8461 acggcgtaga aatcggtgcc ctgctgctga ctggcggcta cgaaatggac gcgcgcattt
     8521 ctaaactgtg cgaacgtgct ttcgctaccg gcctgccggt atttatggtg aacaccaaca
     8581 cctggcagac ctctctgagc ctgcagagct tcaacctgga agttccggtt gacgatcacg
     8641 aacgtatcga gaaagttcag gaatacgttg ctaactacat caacgctgac tggatcgaat
     8701 ctctgactgc cacttctgag cgcagccgtc gtctgtctcc gcctgcgttc cgttatcagc
     8761 tgactgaact tgcgcgcaaa gcgggcaaac gtatcgtact gccggaaggt gacgaaccgc
     8821 gtaccgttaa agcagccgct atctgtgctg aacgtggtat cgcaacttgc gtactgctgg
     8881 gtaatccggc agagatcaac cgtgttgcag cgtctcaggg tgtagaactg ggtgcaggga
     8941 ttgaaatcgt tgatccagaa gtggttcgcg aaagctatgt tggtcgtctg gtcgaactgc
     9001 gtaagaacaa aggcatgacc gaaaccgttg cccgcgaaca gctggaagac aacgtggtgc
     9061 tcggtacgct gatgctggaa caggatgaag ttgatggtct ggtttccggt gctgttcaca
     9121 ctaccgcaaa caccatccgt ccgccgctgc agctgatcaa aactgcaccg ggcagctccc
     9181 tggtatcttc cgtgttcttc atgctgctgc cggaacaggt ttacgtttac ggtgactgtg
     9241 cgatcaaccc ggatccgacc gctgaacagc tggcagaaat cgcgattcag tccgctgatt
     9301 ccgctgcggc cttcggtatc gaaccgcgcg ttgctatgct ctcctactcc accggtactt
     9361 ctggtgcagg tagcgacgta gaaaaagttc gcgaagcaac tcgtctggcg caggaaaaac
     9421 gtcctgacct gatgatcgac ggtccgctgc agtacgacgc tgcggtaatg gctgacgttg
     9481 cgaaatccaa agcgccgaac tctccggttg caggtcgcgc taccgtgttc atcttcccgg
     9541 atctgaacac cggtaacacc acctacaaag cggtacagcg ttctgccgac ctgatctcca
     9601 tcgggccgat gctgcagggt atgcgcaagc cggttaacga cctgtcccgt ggcgcactgg
     9661 ttgacgatat cgtctacacc atcgcgctga ctgcgattca gtctgcacag cagcagtaat
     9721 ctcgtcatca tccgcagctt tgcgctgcgg atatctgaac cggaaataat cactatttcc
     9781 ggttttttat tctcttaatc tgcattaatc ctttctgatt atcttgctta actgcgctgc
     9841 atcaatgaat tgcgccattt cactttgcat acttaccact ttgttttgtg caagggaata
     9901 tttgcgctat gtccgcaatc actgaatcca aaccaacaag aagatgggca atgcccgata
     9961 cgttggtgat tatctttttt gttgctattt taaccagcct tgccacctgg gtagttccgg
    10021 tggggatgtt tgacagtcag gaagtgcagt atcaggttga tggtcaaaca aaaacacgca
    10081 aagtcgtaga tccacactca tttcgcattc tgactaacga agcaggcgaa cctgagtatc
    10141 accgcgtaca gctgttcacg acgggcgatg aacgcccggg cctgatgaac ttcccgtttg
    10201 aaggattaac ctcaggatcg aaatacggga cagctgttgg catcatcatg tttatgctgg
    10261 tgattggcgg cgcgtttggc attgtgatgc gtacaggaac cattgataac ggtatcctgg
    10321 cgcttattcg ccatacccgc gggaatgaaa ttctctttat tcctgcgctg tttattctgt
    10381 tttcacttgg cggcgcggta tttggtatgg gagaagaggc cgtcgccttt gccattatca
    10441 tcgcaccgct aatggtccgg ctgggctatg acagtattac caccgtcctg gtgacctata
    10501 ttgccacgca aatcggtttt gccagttcgt ggatgaaccc gttttgtgtg gtcgttgctc
    10561 aggggattgc cggcgttccg gtgctttctg gctccgagtt gcgcatcgtg gtgtgggtta
    10621 tcgccactat gattggcctg atctttacca tggtgtacgc ctcacgagtg aaaaagaatc
    10681 ctcttctgtc acgcgtgcat gagtccgacc gcttctttcg tgaaaagcag gcggatgttg
    10741 aacaacgtcc gtttaccttt ggtgactggc tggtattgat tgtcctgacc gccgtaatgg
    10801 tctgggtgat ttggggcgtg atcgttaatg cctggtttat tccagaaatt gccagccagt
    10861 tcttcaccat gggtctggtg attggcatca tcggcgttgt tttccgcctt aacggcatga
    10921 cggttaatac catggcttca tcctttaccg aaggggcgcg aatgatgatc gcccctgccc
    10981 tgctggtggg tttcgccaaa gggattttgc tgctggtcgg taatggtgaa gcgggtgatg
    11041 ccagcgtgtt aaataccatc ctcaacagca ttgccaatgc cattagcggt ctggacaacg
    11101 cggtcgcggc ctggtttatg ttgctcttcc aggcggtatt taatttcttc gtgacgtccg
    11161 gttctggtca ggcggcgtta accatgccgt tactggcacc gcttggcgat ctggtcggtg
    11221 ttaaccgtca ggttaccgtg ctggcattcc agtttggtga tggcttcagt cacatcattt
    11281 acccaacctc agcttcgtta atggcaactc tcggtgtatg cagggtggac ttccgtaact
    11341 ggctgaaggt gggcgcgacc ctacttggac tgctgtttat tatgtccagc gtcgtagtga
    11401 tcggcgctca gttgatgggc taccactaaa aatgttaaga gccgcaattg cggctctttt
    11461 ttcattctgc cgtttcagtc tctacggctt catttttggc attgcgcttc atccacaacg
    11521 ccagcgcttt cagcgagtct ggagtgaact catcgcagcg tgcggtgatt tcttccggcg
    11581 tcagccagca aacttcactg acttcatctt cctgtagggc gaagggaccg tgagagacgc
    11641 agctgaacaa cgcgccccag acacggcaat ttttatcttc gaaatagaac tgcccgtgct
    11701 cggcaaaggg gacaccggca atgcccaact cttcttccgc ttcgcgacgc gcggattcca
    11761 gcagttgctc atcggcctga accacaccgc ctgcggtcgc atctaacatg ccgggtaaaa
    11821 agtcttttgt ctcggtacga cgctggacca gaattttgcc catgccatca tgcacgacga
    11881 tgtaagttgc acgatgacgc agacactgtg cccgcatttg ttcccggctg gcttgtgcaa
    11941 tgacttcgtt ctcttcattg acaatatcca cccattcagt acttgccaaa cgacgctgtt
    12001 ccattatcag gaaaccttct ttttctggcg ctcttacggc gcattttgag ttgtgggtaa
    12061 attacggatt aatcgcgacc tgcgcaatga tactttgatc attgagtgcg attacactaa
    12121 gtacgtcatt atccagcatg ccataactcg ccggattacc gcctttcgga atactcaccg
    12181 agccggggtt gaagtggaaa atctcccccc gctgttctgc cactggcaga tgggtatgac
    12241 cgtacaccag cacatcgttc tggtttaaag caggtagatt ttccgggcca aaaagatgac
    12301 catgcgtcaa aaacagacgt tgtttttcca gtaatacctg ttgccacggc gcggttatcg
    12361 ggaaatgcag cagcatttga tccacttcgc tgtcgcagtt gccgcgcaca gcgataacct
    12421 tatgtgccac ttcattaagc cgttcagcga ctttggctgg cgcgtaaccc tccggtaaag
    12481 cattacgcgg gccgtgattc aacacgtcgc caaggatcac cagccactgg gcaccgcttt
    12541 gggcaaacaa ctccagaaca cgttccgtcg ccggtaacga cccatgaatg tccgatgcaa
    12601 acatcagttt catcactcac tcctcgtcga aaaaacgtaa gctatgatac tggattctgt
    12661 cgttgctatc agccagattg cttcgccgaa agtgcaataa aacgctggac agacgctcgc
    12721 tgccactgga atgtcgcata atccaccagg cgttctggca cctcatcgcc atgtagtacc
    12781 aggcggttaa tcattagcgc cagatcagta tcagcaatgc accattcacc aaataaattc
    12841 ggctgaccca gtactaacaa atgttctgcc atcgcaaaca gtttctctgc actggctttt
    12901 ccctcggccg ttagtggcgc ttttttcgcc cccgcaaaga caacatccgt cggacgctct
    12961 tcgcggatgg gcatcagatc gctgcgcagc caggcctgaa tctgtcgtgc acgcgcacga
    13021 ttttctaaat caagcggata aatacgttcc caggtcggtg gcgcaaatcg atcttccaga
    13081 tactccgcaa tggcagaaga ttcactcaac tcaaaatcat cgatttgtaa taacggcaca
    13141 cggcgtgtct gaccgtaacc ttgccacgtc ggctgcaaat gttcaccgct gtcgaggtcg
    13201 atggtcttga tatgaaacga caggcctttt tcctgcaacg ccacccaggc ggataacaca
    13261 taaggggaga aaaagtgggc atctgaccaa agcgtgatag cgggtttact cattatgtcc
    13321 tcggcaataa agggggctgg ctatcaaact atagagcctt tttgtcactg tcacgtgacg
    13381 aatactcacg taagtcgccg cgctttctat acttgtggtg ttcagatgat taaacaaccg
    13441 gagctgcaat gatcgatctc tatttcgccc cgacacccaa tggtcacaaa attacgctgt
    13501 ttctcgaaga agcagagctt gattatcgct tgattaaggt agacctgggg aaaggcggtc
    13561 agtttcgtcc ggaatttttg cgcatttcgc ccaacaacaa aattccggca attgttgatc
    13621 attctcctgc cgatggcggc gaaccgttaa gcctctttga gtctggtgcc attttgttgt
    13681 atctggctga gaaaacagga ctctttttga gtcatgaaac gcgtgagcgc gccgccacat
    13741 tacagtggtt attctggcag gtaggcggac tggggccgat gcttgggcaa aatcatcatt
    13801 ttaatcacgc agccccccaa accattcctt acgctattga acgttatcag gttgaaactc
    13861 agcgtcttta ccatgtactg aacaagcggc tggaaaactc gccctggctg ggaggcgaga
    13921 actacagcat tgcggatatt gcctgctggc cgtgggttaa tgcctggact cgccagcgaa
    13981 ttgacctcgc aatgtatccg gcagtcaaga actggcatga gcggatccgt tcgcgccctg
    14041 ccaccgggca ggcactgcta aaagcacaac tcggtgatga gcgttcggat agttaacaga
    14101 aacaggttct cgtgtattat ttcatcctaa gtaaaacaac ggagaacctg caatggcaca
    14161 acctgccgct attattcgta taaagaacct tcgtttacgt acgtttatcg gaattaagga
    14221 agaagaaatt aacaaccgtc aggatattgt tatcaatgtg acgatccact accccgccga
    14281 taaagcgcgc actagcgaag atatcaacga tgcgctgaat tatcgcaccg taacgaaaaa
    14341 cattattcag catgtagaga ataaccgttt ctctttgctg gaaaaattaa ctcaggatgt
    14401 gctcgatatc gcacgtgaac atcactgggt gacgtatgct gaagtggaga tcgataaact
    14461 gcacgcgctg cgctacgccg attcggtatc catgacctta agctggcagc gttaaccgcc
    14521 atatcgggag gctgcatgaa catagtgatc accggaggga cgggattaat tggtcgccat
    14581 ttgattccgc gtttgctgga gctgggccat caaatcacgg tagtgacgcg taacccgcag
    14641 aaagccagtt ccgttctcgg ccctcgggtg acgctatggc aagggctagc cgatcaaagc
    14701 aacctcaacg gcgttgatgc ggtaatcaac ctggccggag aaccgattgc tgataaacgc
    14761 tggactcacg agcaaaaaga gcgtctctgc caaagccgct ggaatatcac gcaaaaactg
    14821 gtcgatttga ttaatgccag cgacacgcca ccgtcggtac tcatttccgg ctcagcaacg
    14881 ggctattatg gcgacttagg tgaagtggtg gttaccgaag aggaaccgcc gcataacgaa
    14941 tttacccata aactctgcgc ccgctgggaa gaaattgcct gccgggcgca aagtgacaaa
    15001 acgcgagtgt gcctgctgcg taccggcgta gtgctggcac cggatggcgg tattctcggt
    15061 aaaatgctgc cgccgtttcg tcttggcctg ggcgggccga ttggttccgg tcggcagtat
    15121 ctggcctgga ttcatatcga tgatatggtc aacggcattc tctggctgct ggataacgag
    15181 ctgcgcgggc catttaatat ggtttcgccc tacccgatac gcaatgaaca atttgcccat
    15241 gcgctcggtc atgcgctgca tcgcccggct attttgcgcg tccccgcaac cgccattcgg
    15301 ctgttaatgg gcgaatcttc agtactggta ttaggtggac aacgcgcgct gcctaaacgg
    15361 ctggaagaag cgggttttgc gtttcgttgg tacgatttag aagaggcgct ggcggatgtc
    15421 gttcgctgat gtggtttact gcaaacatcc gccagttaac tcccggtgtt acagggttag
    15481 tggctttgcg cgataagatc gtctggtgaa agtcgggtca ccatcagaac taactctctg
    15541 tctaaacctc tatccagcat ctcctgagca atacgcaggg cttcttcgtg tttgccctgc
    15601 attgcgcctt cttcacgtaa tctgtcagca atggtcatca gtttctcctt ttcttgtggt
    15661 gcgcgttctg ttatctcacc aataaatgca cggaaacgct gggcatcccc tgtttgtaat
    15721 acgtaattaa acagggcttt tagctgtctg tcattagtgt tccctgtaac tagcagcgaa
    15781 acaatttggt cgactaatcc caacagatcg cgctgacgaa tatgtttctg aattaactcc
    15841 aacagcgcca ttttgcggtg ttgcataatc tcgtcatccg gcaccacggt aatatccacc
    15901 aacggaaacg ctgatgaata tattttgcgg gctatagcag gctcggcaaa ttcatcaagc
    15961 cagcagagtg aataaggata aggacttctg caaccatgat aaaacagcat cgggatcacc
    16021 aatggaagct ctttatagcc cgcatcaaga tggttttgca ttgccgcaat ggaataacgc
    16081 atcatgcgaa aagccattaa ttcttccggc ttactttggt gctctatcac tacataaata
    16141 taacccactc cctcctgcgt tttcacagac cacaagaggt cggaataata ttgccgcagg
    16201 tcttcatcaa taaaactgtt tggttccagt ttaagcgtcg ttaaatcaca cagtttgcgc
    16261 agcggcgcgg gaagatgaat atcaataaaa tcccgcgcgg tgtctggatg gcgtaaaaaa
    16321 gatttaaata ccgcatcatg cggcgtggaa gttgtcgata ttgtcatggc gttccgtcac
    16381 ccctcaaatt taagatgacg cgacaataac cacgattaac acccaatgca gcaggcagtt
    16441 tttatctttg gacagcgaat tcagaagtat ttttagcgga ctgcaacttt tacatttcag
    16501 cggaagctga ctcgcaaatt ccccatgaaa atactccggc agcgttagcc accggagagg
    16561 aaattatttc agcgatccct taaggaactg ttgcagacga gggctttgcg ggttgccaaa
    16621 taactgctcc ggcgcgccct cttcttctat tttcccctga tggaggaaaa tgacatgagt
    16681 agaaacatgg cgagcaaagc ccatttcgtg agtcactacc accatggttt tcccctcttc
    16741 tgccagttgc tgcataatac gcaacacttc gcctaccagt tcaggatcga gcgccgaggt
    16801 aggttcatca aacagtaaaa cttccggttc catcgccagc gcccgcgcga tagaaacacg
    16861 ctgttgctga ccgccggaaa gatgcaccgg atatttcccc tgcgcacgtt cgtctatccc
    16921 gacttttgcc agatacttga ccgcccgctc gcgcgcttcc tgcttgctca ggcccaacac
    16981 ctgaatcggc gcttccatga cgttttccag caccgtcata tggctccaga gattgaagtg
    17041 ctggaatacc atcgtcaggc gtgtgcgcag taagcgcagt tgatttttat cggcgacttt
    17101 gagttgaccg tctttgtcgc gcaccagatt gatcgtctgg ccattgacca cgatcgaccc
    17161 ttcactcggt ttttcgagga agttaatgca gcgcagaaag gtacttttcc ccgatcccga
    17221 cgatccgatg atgcttatta catctccggc attcgcttgc agtgagaccc ctttcagcac
    17281 ttcatgttcg ccgtagcgtt tgtgcaaatc gataacgttt aatttattct cggacatcgt
    17341 attctcagtg cgttgaagaa ggtttcacat gctgcaacca gcgtttttcc gctctgcgaa
    17401 agaggctgat caggacataa gagatgatta aatagagcac cgcggcaatg ccgaaggcgg
    17461 taaaaggttg atacgtggcg gcgttaatat cgcgggctat tttcagcaga tccggcacag
    17521 tggccgtaaa cgccagcgca gtagagtgca gcatcaggat cacttcgttg ctgtatgccg
    17581 gtaacgcaat acgcagcgca gaaggcaaaa taatgcaacg atacatttta aaagtcgaga
    17641 agccataggc tctggcggct tcaatttccc catgcggaac cgaacggatt gccccagcaa
    17701 aaatctcggt agtgtaagcg caggtgttaa gcgtcagcgc cagcacggta cagttcaggc
    17761 cactgcggaa gaaagcgtta aggaattcgg ttcccttaac aatctcaagc gtgtacatgc
    17821 cggaatagaa caccagcaac tgaacataca gcggcgtacc gcgaaaaata taggtaaata
    17881 accagattgg aaactggatg tatttattac tggagacacg accaatcgcc agaaacagcg
    17941 ccaggactcc gcctatcact accgacaaaa taagcagcca cagagtgatc gccacaccag
    18001 taaagcgata accgtcggtc cacagcagcg gtttccagta ttcatgtaag atttcgatca
    18061 caggtcagcc ctcttcacac ccacggagta gcggcgctca aggaacagca gcacaccatt
    18121 ggaaacagtg gtgaaaacca ggtaaatcac gccacagacg atggcgaaat agaacggttc
    18181 ccaggtactt ttgcctgcca gttgggtggc tttgaccaca tcttccaggc cgagtaacga
    18241 aaccagtgcg gtagatttga ggatcacctg ccagttgttg ccaatgcctg gcagggcgta
    18301 acgcatcatc gccggaaaca tgatccgccg aaacacttgc ccacgagtaa aaccgaacgc
    18361 cgtcgccgcc tctatatgcc ctttcggcac tgccataaac gcgccacgga acgtttcggt
    18421 aaaataagca ccgtagataa aaccgagagt aataatacca gcgaccatcg gatcgatatc
    18481 aatctgcccg acgcccatcg cctccgtcac cgtgtttagc gcaatctgca aaccgtagaa
    18541 aatcagcagc atcaacacca agtccggcac gccacgaatc agcgtggtgt acccttcgaa
    18601 aatcagcccc gaaagccgat tttgcgagag cttaccgcca gcgccaatta aaccgatgat
    18661 tacagcgagc actacagaac tgatagccag ctccagcgtg acgagcgcac cctgtaaaat
    18721 aacacctgaa aacccataca acatgctgcc tgtcctgtcg tgtgtggtga attaccgcct
    18781 cttgtctccc tccggttacc cgaagggagc gggctttctt tcaccgatgg cgattaaccg
    18841 ccataaacat caaaatcgaa gtactttttc gctaatttct cgtaagtacc gtcagcgcgc
    18901 atttcggcaa aggctttgtt cagtgcctcg cgcagttcgt tatcttcttt acgcaggccc
    18961 atgccggtcc ctacgccaaa cagtttttca tctttaacag acgggccacc gaatttgtaa
    19021 tctttaccga cgggttgttt gaggaaacct tcactggcag cgacctcatc ctggaacgcg
    19081 gcatcaatac gtccggcagt caggtcagaa taaatgttgt cctgcccctg atacgagacg
    19141 atttcaatgc cttttggtgc ccagtgttcg ttaccgaacg tctcctgggt ggtgccctgc
    19201 aatacgccta cccgtttgcc tttcagcgac tcgactgtcg gctgaatgtc ggaatttttc
    19261 gccaccacca aacgagaatc ggcagcgtac agtttgtcgg tgaaggctat ttcttgctga
    19321 cgtttttccg taatggaaag cgatgacatg atggcgtcaa tcttcttcgc ttttaaggac
    19381 gggattaacg catccagcgg attttcgaca aacgtacatt gcgtattgat gcgtttgcat
    19441 aattcctttg ccagatcgat atcgaagcca accagttcgc cttgtgaatt ttttgattca
    19501 aatggcgcat aggtcgggtc ggtaccgatg cggatgtttt gcggaatcgc agcaaatgcc
    19561 gcagttgcgc tggagaaggc cagaaccaga gagagcgata gcaccagttt tttcataatg
    19621 tcctcaactg acagtctttt tatagggaat atttacaggt ttgttacact tatcgtgcca
    19681 taaaattgac catcaaggca aaatatcccg ccccgttaga cgtattattt ttcattaaaa
    19741 atgcttaagt atgcatttca atgcaccacg atagtgcaac aaacctggcg tgcaccatga
    19801 tgatcattcc atcaggtaca gcttcccagc gacgtatcag tcaccgtaga cattaaagtc
    19861 gaaatacttt ttcgccatct tgtcgtaggt gccgtcctga cgcagctcgc caagcgcctt
    19921 attgaaggca gccgtcagtt cagcatcatc tttacgtagc cctacaccgg tgccatcacc
    19981 gaagtatttt ttgtctttta ctgatgagcc agcaaaggcg aaatctttac cagcaggttg
    20041 cttgaggaat ccttcgctgg cagcaacttc atcttgtaac gcagcatcca gacgtcctgc
    20101 agccagatcg gaatagacca aatcctggtt ggcataggcc accacatcca cgcctttgct
    20161 acgccaggtc tcgttagcgt aagcttcctg ggttgatccc tgcagcacac caacatgttt
    20221 acctttcagt gaatccagcg ttggctgaat cggtgaacct ttggcagcaa tcaaacgaga
    20281 atctgcggcg tacagcttgt cggagaaggc aatctcctgc tgacgtttat cggtaatgga
    20341 aagcgacgaa ataatggcgt cgattttttt cgctttcagt gaggggatca gcgcgtcaaa
    20401 gtcactggca acccaggtac atttcacctg catccgtttg cacatctcgt taccgagatc
    20461 gatatcaaag ccaacaaaat cacctttagc atctttcgac gagaacggcg cataggtggt
    20521 atcggttccg atacgtaccg tctccggtag cgccgcatag ctggaagccg ctgcggagag
    20581 accgactaac aaagacagag cgagaatcga cttcttcata cataaccctc aagtgaaaat
    20641 ggctttttta tgttgtgtat tgtgttgtgt gtttgcaggc tctttcatgc aggtcttatg
    20701 ccatcttgac gacaacagta acattcaacg ttaaatatgt taataagacg ttgcattatt
    20761 gtcctgaagt tgaagatagc aggtatagcg gttgaatcgc agcgtttcga ttgaatggca
    20821 gaaacaaaat gtcgaggatt tgatcgcggt tgcaaaattg ccctgaaaca gggcaacagc
    20881 ggagttatgc gccctgccag cgggcaaaga gatcttcagg aagggttatc gcaaactggt
    20941 caagaacacg attaaccgtc tgatttatca catcatcaag ggattgcggg cgatgataaa
    21001 acgccggaac gggaggcata atcaccgcac cgatttctgc cgcctgagtc attaaacgca
    21061 gatggcctaa gtgcaatggt gtttcacgca cgcagagcac caacgggcga cgctctttca
    21121 gcaccacatc tgccgcacgg gtcagtaaac catcggtgta gctatggaca atgccggaaa
    21181 gggttttgat tgaacagggt aaaataacca tccccagcgt ctggaaagaa ccggaagaga
    21241 tgctggcggc aatatcgcgc gcatcgtgcg tgacatcggc taatgcctgc acttcgcgca
    21301 gagaaaaatc cgtttcgagg gataaggtct ggcgcgctgc ctggctcatc accagatgcg
    21361 tttcgatatc tgtgacatcg cgcagaacct gtaataagcg cacgccataa atcgcgccgc
    21421 tggcaccgct gatgcctaca atgagtcgtt tcataaatag ttgccctgtc agacttgcag
    21481 gcagactttg caggatttcg gcgggagttg caagtcaggg tgccagaccg gcaccctcag
    21541 cgaaggcatc atccttcgtt atgcatttcg agattttcca cttcgttctg acgttgcact
    21601 gctttggcgt catcattacg taacgtatcg aggaaatcga ggtagccctg atcaacatct
    21661 ttggtgacgt agacgccgtt gaacaccgag cattcaaact gctggatatc cggattttca
    21721 gcgcgaacgg cgtcgatcag atcgttcaga tcctggaaaa tcaacccgtc agcaccgatg
    21781 atctggcgaa tttcatcaac ttcgcgaccg tgagcgataa gctccgtggc gctcggcata
    21841 tcaataccat aaacgttcgg gaagcgaatt tccggtgccg cagaagcgag gtacactttc
    21901 ttcgctccgg cttcgcgtgc catctcgata atctgctcag aagtggtgcc acggacgatg
    21961 gagtcgtcga ccagcaggac gtttttatcg cggaactcgg cgcggttggc attcagttta
    22021 cggcgcacgg acttacgacg cagctgctgg cccggcatga taaaggtgcg gccaacatag
    22081 cggtttttaa cgaagccctg gcggtacggt ttgcccagaa tacgagcaat ttccagcgcg
    22141 atatcacacg aggtttctgg gatcgggatc accacgtcga tatccagatc ttcccattcg
    22201 cgggcaattt tctcgcccag tttcgtgccc atattcacac gcgcgctgta aacggaaatt
    22261 ttgtcgatga acgagtccgg gcgagcaaag tatacatact caaacaggca cggattgctg
    22321 accggattgt cagcacattg acgggtaaac aactgccctt cttcagtgat gtaaatcgct
    22381 tcgcccggcg cgacgtcacg caggaaatca aagcccagcg tatcgagcgc tacgctttcg
    22441 gaagcgacca tatattctgt acggttctcg tcaatatcac gttttcccag taccagcgga
    22501 cgaatcccgt ttggatcgcg gaaagcaacc ataccgtggc cgataatcat cgccacacag
    22561 gcatacgcgc cgcggattaa gcggtttgtg gcagcaatgg cagcgaaaat attgtcggct
    22621 tccagcgggt agtggcggaa attgtccagc tcgctggcga agatattaag cagaatttcc
    22681 gagtcggaag tggtgttgat gtggcggcgt ttttcttcaa acagtttttt acgcaactcg
    22741 tgagcgttgg tcagattgcc gttgtgggca agcgtaatgc catacgggga gttaacgtaa
    22801 aacggctgcg cttcagaggc gctggagctg ccagccgtag ggtaacgcac atgaccaatg
    22861 cccatattgc cctgcaaacg ctgcatatgg cgagcttcaa atacatcgct caccagcccg
    22921 ttcgctttac gcaaacggaa gcagttattg gcatctatgg tgatgatgcc ggcggcatcc
    22981 tgaccgcgat gctgaagcac cgttaaggca tcataaatcg actggttaac cggcataaca
    23041 ccggcgatac cgacaatacc gcacatacgt ctttttcctc gttaagccac atctcagagc
    23101 acttacgctc tgggcaagaa acttgacgag ctttgcagat aatcaaaaaa ccatctgatg
    23161 ataaaactga attgcgggat cagctgtgat ttgctccagt cttcgctttt cgacaccccg
    23221 gtaaaggagt cgagaaagaa gagaatggcg gcaacaatca acacaccgcg caacgcaccg
    23281 aaacagacgc ccagcacccg atcggtgcct gacaaccccg ttttctccac caactggcct
    23341 atcacgaagt tcacgatagc accaacgatc agggtagcga taaacagtac cgcgatggca
    23401 atcccatttc gaaccagttc gtcttcaaag cccgtaaacc agactgacag gtaagtgtag
    23461 taatgactgg caacaaagaa agcacaaccc catgtcacca gcgataacgc ttcacgaaca
    23521 aagccgcgga tcaggctaac cagagaggaa aaagcaatca ccgcgattat ggcgtaatca
    23581 atccagacca tatgtgtccc acgattttac gccctgtcat cctgttcggg gcgcattcta
    23641 acagaaaaag aaaacgtttg cgtagggatt tccttcccac gcatcaataa aaatggcgct
    23701 gaaaaaatat tcaacgccat cgacttttta tgcctttgcg gcatcgtgca atgcgtgtcg
    23761 gatgcggcgt aaacgcctta tccgacctac ggttctaaca ctgcccaggc ctgataagac
    23821 gcgccagcgt cgcatcaggc aagactgtat taattcggcg tatagcccat taccacgcca
    23881 cttaagccag aaagttgctt caactcaccc agcgaacctt tcagcttatc tttcgaggca
    23941 tccggcccaa ccagaatacg ggtaatttta ccctgcactg gcgtggatgg cgacgtataa
    24001 acccgataac cggcaccgcg cagcttaccg acaatctcat tcactttatc ggcatttttc
    24061 agcgcaccca gttgcacaac ataggcttta cccgtcggtg cagctttttc ttccacgacc
    24121 ggcttcggtt ctggcttcgg cgcaggtggt gcttcaacct ttggtttagg cggctccacc
    24181 ggtttcggct tcggtggggc gaccggtgca ggttccggtt caaactcggt gttattggct
    24241 gcaatagtgg ccggatcgag cgacggtgct gcggcatcac ctgcccgcac ctcttccgct
    24301 gcgccttccg gcggctgcgt cggcaacgcc tgggtggcgg ctggcatcat atcaggctca
    24361 tcgcgatcgc ccgcttttgg aaccagcggg atagccgcaa actcatcctg ataatgtttt
    24421 ttctgcccgt ccagcagccc tggaagtaca atcaccccca gcgccaccag cacgatcgtg
    24481 cccactaacc gattctgaaa cttacttgcc accgcttctc ctcgcgtcaa tcacttccat
    24541 gacatgtgcg accgtgtgga aagaaccaca caccagcacg gtgtcttccg ctttagcgtc
    24601 cgccattgcg gcatcccatg cctgcgcaac gctatcaaat gatttgccgt tacccaaatg
    24661 ctcaagcagt tgttctgccg tggcaccgcg cggcccttcc agtggcgcac aataccagtc
    24721 atcaaccacg cttttcaacc aggccagagt tccggcaata tctttatcat gtagcatacc
    24781 gataaccgcc agcacgcgcc cgtttttcgg taaggctttc atacgcccgg tgagatattc
    24841 cgccgcatgt ggattatgcg cgacatcaaa aataacgcgt ggcgactcgc tcacaatctg
    24901 gaaacgtccc ggcaaaattg cgctggcaat cccgtcgcga atggcatttt cactgacctc
    24961 cagcccgctg gcacgcagtg ccgccagcgc cgttgcggca ttcggttgcg ggacaagcgg
    25021 caacggcaaa ttttccagcg tgccgtgagc atcgctaaac gtccagtcat gatcggtgac
    25081 ggaatagttc cactcaacgc cccgacgttg taacagtgca cctttttcct gcgccacatc
    25141 agcaatggta gaaggcattt ccggctcacc gacaattgcc ggtttttcgc tgcgaaagat
    25201 gcctgctttc tcgcgaccaa tactttcgcg atctggcccc agccagtcgg tatgatccag
    25261 cgcaatgctg gttactaccg cgacatcggc gtcgacaata ttggttgcgt ccagacgacc
    25321 gcccagccct acttccagaa tcaccacgtc aagttgtgcc tgtttgaaca gccacaacgc
    25381 cgacagcgta ccgtactcga aataggtcag ggaaatatca ccgcgtgccg attcaatctc
    25441 cgcaaaagag gcggtgtggg ccgattccgg caattcctga ccctgcacac gtacgcgctc
    25501 ggtataacgc accagatgag gcgaactgta gacgcccact ttgtaccctg ccgccatcag
    25561 aatcgactcc agcgtacggc aggtggtgcc tttgccattc gtacccgcaa cggtaaacac
    25621 aaatggcgct ggtttcagaa cgccaagacg cgccgcgacc tggctcacgc gctcaaggcc
    25681 gagatcgata gttttactgt gcaggttttc cagataagaa agccacgaag ccagaggcga
    25741 cgcggcttga ggagtgcgtt tgataatcat ggtatccgct gattcgttac ggtgagaata
    25801 gcaaaagggc agagccagtg gccctgccct tatcagttat caggcctcag gttcctgatc
    25861 cggtaccggg ggtaccacta cgccttcacg cggcgcttca ggattcggcg ctggcagatt
    25921 catcaacttc gccagaatgc tcgccagttt caggcgcatt tccggacgac ggacgatcat
    25981 gtcgatcgcg cctttctcga tcaggaattc actgcgctgg aatcctggcg gcagtttttc
    26041 gcgaacggtc tgttcgataa cacgcggacc ggcaaagccg attaacgctt tcggttcagc
    26101 gatgttgaga tcgcccagca tggcgaaact tgcagaaaca ccgcccatcg tcgggtcggt
    26161 cagcacggag atgtacggca agccgcgctc ctgcattttt gccagtgccg cagaggtttt
    26221 cgccatctgc atcagcgaca tcagtgcttc ctgcatacgt gcgccaccag aggcggagaa
    26281 gcagatcagc gggcagttat cttccagcgc ctgctcaacg gcacgcacga aacgtgcacc
    26341 cacaacagac cccattgaac cgcccataaa ggcgaactca aatgccgcag cgacaaccgg
    26401 cattccatac agagtgcctt tcatcaccac cagcgcatct ttttcgccag tttctttctg
    26461 cgcagatgcc agacggtctt tatacttctt ggagtcacga aacttcagca catctttcgg
    26521 ctcaagctcg ctacccagct ccacaaggct tccttcatct aacaggctat gcaggcgatt
    26581 acgcgctgtc atacgcatgt gatggtcaca cttcggacag acctcaagat tacgttccag
    26641 ctcagcgcgg tataaaacct gaccgcagct atcacactta gtccacaccc cttcaggaat
    26701 gctcgccttg cgggtgggag taatgttgct tttaattcgt tcaatccagc tcattaggga
    26761 cctttctgtc tgaacctggt tcgatgccag ttttatcttt ggggacgcat aatgccattt
    26821 ttgcccccaa cagaccatga atgttgcaca ttaaaacata acagcccgaa actttggata
    26881 aaaaagtggt cgaaccgcgg agttactttt tattttgcgg cgcgtgccgc agcgcgtttg
    26941 tgacgaataa attcgaaaac cccaggcaat attgaaacaa aaataatgcc aacaatcatc
    27001 agcttaaggt tgctctgaat gaaggggagc gtgccgaaga aataacccgc gtaggtaaaa
    27061 atcagtaccc acaacagtgc gccgatcacg ttataagcgg cgaaatgacg gtacgacatg
    27121 tggcccattc ccgcaacaaa cggcgcgaac gttctgacga tcggtacaaa acgggcgaga
    27181 ataatcgttt tgccgccatg tctctcgtaa aactgatgag tcttatcaag ataactgcga
    27241 cgaaagattt tcgaatccgg gttactgaac agtttgtcac caaataaccg tccaatggta
    27301 tagttcactg catcaccgac aatagcggca atcaacataa gcaccaccat catatggaca
    27361 ttgagatcgt tagtttccag cgacgccaat gcaccagcaa caaacagcag cgaatcacca
    27421 ggcaggaatg gcgttaccac cagccccgtt tcgcagaaca gaatcaaaaa cagaatggca
    27481 taaacccaga cgccgtactc cgcgaccagc tctgccagat gcacgtcaat gtgcaggata
    27541 aaatcgatga ggaaataaat caggtccata tttgcctatg ccttgtactc gtcatctttc
    27601 aggctgtaac tacgttggct acagcctgaa atgttccgag cattattctc gttagtccgc
    27661 cagaaatagc gggcccattg gcggttttgg aagatcatac cggtcagggt aatccaccgc
    27721 gaccagatac agcccttccg cttttgccgt tgctgccgcc agcgttctgt cctttgccgc
    27781 cagcaactct gctatccagc tctccggctg gttgtgggca ccgacttcca tcaggctgcc
    27841 gacaatattc ctgaccatat gatgtacaaa ggcattcgct ttgatatcta ccaccacata
    27901 aggaccgtga cgcgtgacat taatatgcat aacgttacgc cacggggttc gggactggca
    27961 ctgcaccgca cggaacgagg tgaaatcatt ctcgcccagc aagcattgcg cagcccgatg
    28021 catccgttca gcgtccagcg gttcgtaaaa atgggttacc cctttactca gtaccgccgg
    28081 gcgcagccga tgattgtaga tgatgtagcg ataacggcga gccgtggcgc taaatcgggc
    28141 atgaaaatca tcaggtacag ctttaaccca acgcacggcg atgtcaccag gtaaattcgc
    28201 atttacgccc aaggtccacg ccgcgtcttt gcgctgcgcg gttgtttcga aatgcaccac
    28261 ttgcccggta ccgtgtaccc ctgcgtcagt acgcccggcg cagaagacgg tgatgggttc
    28321 gttcgccacc tgggagagcg ccttttccag cttctcctgc acactgcgga cttcattctg
    28381 ccgttgccag ccgtaatact tactgccgtc gtactcaatg cccagcgcaa ttttataaac
    28441 tggcagttgt tgctggtcgg acattagtac agatactcct gcaccagttt ctcggcgatt
    28501 tttactgcca tcagcgcgcc gccaaagcga acgttatcgg ccaccgacca gaactggact
    28561 tgctccggca taccgtagtc attacgcacg cagccaacag aaagatgcgg cgtacccgaa
    28621 gcatccccta cctgagtcgg gaattcgttc tcttcagaga gcacaatatc ttcgccctga
    28681 gcaaacgcat cacgcgcttc ttctgccgcc agcggacgca gtgcttcaaa gttgaccatc
    28741 tgggcatgac cgtagaatac cggtgcctgg acgacgcttg ccgaaatcat cagcccttcg
    28801 tcctgcagga ttttgcgtac ttcgtcaacg atacgacgtt cttcacgcac gctaccttcg
    28861 ctatccggca acaacggcag catattgaac gccagttgac gaccgaagaa atcttcttcg
    28921 tcaatcggaa tgccgttgag caatttcgca ctctgccccg ctaacgcatc gaccgctttt
    28981 ttgccctggg cggaggctga aatcaggctg gtaacactga tacgcgacaa accgccctga
    29041 tcgattaacg gtttcagtgc cgccagcagc tggctggtca gactgtctgg tacggcgatg
    29101 acattccggt tgcggtaatc ggtcagtaca aacgggttta cttccggcac caccagcggt
    29161 acgtcgggtt cgagagcaaa cagtccgctg ctgtcgatca ccaggcaacc tgagttggtc
    29221 gcttcttcaa cccaggcagc ggtagcttct ttgcctgcga caaaaaatgc cagctgcgcc
    29281 tgcgtccagt cgaattcagc ggcatcctgc acggtgattg ttttaccacc aaagcgcagt
    29341 tgttcgcctg cgctttcgtt acgtgccagt gcataaattt ccccaaccgg gaactgacgt
    29401 tcagccagcg tttcaagcag ggcttcgccc acagcgccag ttgcgcccag gacggcaatg
    29461 ttccagcctt cagacatggt ggtttactcc agaaatagca aagctccctg ccaatgttac
    29521 agcagagagc atgaagaaga gattaacgtg ccggatgatg aacggcgtta aaacccagtt
    29581 tacacagcaa tgatgccgca ctggcgtcat cacaaattac atacagagat gaccattcac
    29641 ggcgctcaag atagtttttg cgcagtttat cgaactcacc cggtatcccg gcgactttac
    29701 gcagcggtgc gtcatcgcgg cgcacatcat acaccaaatg caccagcctt ttcagcgtcg
    29761 gttgatcgag cgggccatgc agcgtaatgc gaccaaactc tggcgcgggc agtaatgtat
    29821 ccagcgcaac gtgctgttca tgcccaataa acttgctata ggcttcaaac acttgcgtag
    29881 taccgcgtgc tttaccttcc agggtataac ctgcgatatg cggcgtgccg atatccactt
    29941 ttgtcagcaa ctccacgtta agttccggtt cgccttccca gacatccagc actacgctta
    30001 acttctggcc ttcattcagg caggtcagca acgcagtatt atcgacgact gcgccacggc
    30061 aggcgttaat cagaatcgct cccggcttca ggctacggat cagtttttca tccgccagat
    30121 gcagcgtttt gtacggccca tctttgaaga gtggcgtatg gaaagtcaga atatccgcat
    30181 gctgaactaa ttcatccagc gagcggaaat caccctcatc cccacggtca gcgcgaggcg
    30241 gatcgcaaag caatgtttta atccctaacg cttccagtcg cgcctgcaat cgacgtccaa
    30301 cgtaaccaac gcccacgatc cccaccgtac ggtcgtgcag tgaaaatcca tcgcgttcgg
    30361 caagcatcag tagtgaggag aaaacatatt ccaccaccgc aatcgcatta cagccaggtg
    30421 cagcggaaaa accaattccc gcctgcttca accatgcttc atcgacatgg tcggtccccg
    30481 cagtggcagt gccaacaaat ttaatgggtt ttcctgccag caaagattca ttcactttcg
    30541 tgaccgaacg caccatcagc gcatccgcgt ctgccagttg agcgacgggg attggacgcc
    30601 cgggaaccgc ggtcacctca cccaaacggc taaataagtc gcgggcataa ggcatatttt
    30661 catcaacaag gattttcacg tttgtgttac ctgtatgaga cgagagttaa ccggacaagt
    30721 gtgccataat ctcgcggcca ggcatacttg cgaagatttc aggtataagg atacgtaatg
    30781 atacaaccta tttccggccc tcctcctggg caaccaccag gtcagggaga taacctgccg
    30841 tctggcgcgg gcaatcagcc tttatccagt cagcaacgta cttcgctgga aagcttaatg
    30901 acgaaagtga cctcactgac gcaacagcaa agagcagaac tgtgggcggg tatcaggcac
    30961 gatattggtc tgtcgggaga ttcaccgctg ctttcgcgtc acttccctgc cgctgagcat
    31021 aatctggcgc aacgtctgct ggccgcgcaa aaaagccatt ctgcccgcca gcttttagcg
    31081 caattagggg agtatttacg tctggggaat aatcgtcagg cggtcacgga ttatatccgt
    31141 cataactttg gtcagacgcc gctgaatcag ctctcaccgg agcaattaaa aaccattctc
    31201 accctgttgc aggaagggaa gatggttatt ccgcaaccac agcagcgcga ggcgaccgac
    31261 cgtcctttat taccggcgga gcacaatgcg ctaaaacagc tggtgaccaa acttgcggcg
    31321 gcaacggggg aacccagcaa acagatctgg caatcgatgc tggaactttc cggggtgaaa
    31381 gatggcgagt taattccagc gaaactgttt aaccatctgg tgacctggct acaggcgcgt
    31441 cagacgctaa gccagcaaaa tacgccgacg ctggaatcac tacagatgac gctaaaacaa
    31501 cctttagatg ccagtgaact ggcggcgtta tcggcatata tccagcaaaa atatggtctt
    31561 tctgcgcaat catcgctttc ttctgcccag gccgaggata ttcttaatca gctttatcaa
    31621 cggcgggtta aagggattga tccgcgtgtt atgcaaccgc tgcttaatcc ttttccaccg
    31681 atgatggaca cgttgcaaaa tatggcaacg cgtcccgcgc tgtggatact gttagtcgcg
    31741 attatcctga tgctggtctg gctggttcgt taaccccgac gaaatgacag tatcgtgaca
    31801 ataataccca gcaccgcaga gatcgccccg gcaagaaata ccgaagagta accaaacgtg
    31861 gtcgccagca ttcccgccag cggcccggag acgccgaggg cgatatcctg aaacgcggcg
    31921 taaccgccca gtgcggtgcc gcgaacttgt gaggggacgc gtttaaccac ctccacgccc
    31981 agcgcaggaa agataagcga acatccggct ccggttaacg ccgcgcccgc taatgcgacc
    32041 catgcacctg gggcttgcca gagcagcaac aagcccaccg tttctacaag cagagagaca
    32101 atcgccactt tcacgccgcc aaaacggtcc ggcatccagc caaacatgac gcgcatcacg
    32161 acaaatgcgc cgccaaacgc ggtaagagta aagcccgcca tcgcccatcc tttgctggca
    32221 aagtagagcg aaacgaaagt cccgataaca gcaaaaccaa cgccttgtag tgccagacct
    32281 aaccctggtt tccagataag cccgacaacg ctccacagcg atggacgttc tcccgccagg
    32341 gccggtactt tgcgcactgt gccgttacag gcccacgcca gtacgggtaa taccattgtg
    32401 gtgatcgcca gtgcggcaaa accgtaatgg ctatgaatca acaggccaag cggagcacca
    32461 acagcgaggg caccgtaaat cgccattccg ttccatgaca tcactttgcc agagtgtttt
    32521 ggccctacga tgcctagtcc ccaggtcaga gcgcctgtca gtaactggct ttcaccaaag
    32581 ccaagaatca aacgcccgac gaccaacaga gcaaatttga acggtgcgga gacaggcaaa
    32641 atcgccgcca gcagcaacgc gccgccagcc agaccacaag ctaacattcc ctgaagcgcc
    32701 gaacgttttg caccatattg atcggccagt cgcccggcgt aaccacgcgt cagcaccgta
    32761 gccagaaact gaatcccgac ggcaatgccg accatggtat tgccatagcc cagttcatga
    32821 tgaacaaaca gcgggataac cggcaacggc aaccctacgg tcatgtaggt gagaaaaacc
    32881 gcaaaagcga tgcggaagag cgaaaaattg gcagaagatc gtgtttcggt ttggcttaca
    32941 gcagtcatgc attactccag aatgcagcgc aaggcgagga gtatccccgt ctcatctctc
    33001 tggtttcagg gttacggtgc gttggcagga tttaacgcgt acgtcttttc agaaggaaat
    33061 cgacaaagcg ggaagtttgc ctggaactgg cggcgattgt caatgatgtg aaaaagggaa
    33121 ccatcaggtt cccttttgcg ttagtgccgg aggccgcatt ggcgcgtaac gtcggatgcg
    33181 acgctggcgc gtctactccg acctactgcg aattaatctt tcagcttgcg cattaccagc
    33241 gtggcgttgg tgccgccgaa gccgaagctg ttagacataa cggtggtcag ttcgcgatcg
    33301 gtcgtttcgg tcacgatgtt cagacccgca gcctgctcgt ccagctcttc aatgttgatg
    33361 ctcggggcga taaagccgtg ttccagcatc agcagagagt agatagcttc ctgtacgcca
    33421 gcagcgccca gagagtgacc ggtcatggct ttggttgcag aaatcgccgg gctcttatcg
    33481 ccgaacactt cacggatagc tgccagctct ttcacgtcgc caaccggagt cgaagtaccg
    33541 tgggagttca ggtaatcgat tggggtatca acgccatgca tcgccatctt catgcagcgt
    33601 actgcgcctt cgccagacgg agcaaccatg tctgcaccat cagaggttgc gccgtagcca
    33661 acgatttcag catagatgtg agcaccacgc gccagcgcgt gttccagctc ttcaaccact
    33721 accataccgc cgccgccagc gataacgaaa ccgtcacggt gagcgtcgta agtacgggag
    33781 gctttttccg gggtgtcgtt gtatttagta gatagcgcac ccattgcgtc gaattcgcaa
    33841 gccatttccc agcacagctc ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt
    33901 tggatctgct ctactgcgtt accgatacag tgtgcggaag tcgcacacgc ggagctgatg
    33961 gagtagttaa cgccatgaat tttaaacggg gtggcgaggc aggcagaaac gccggatgcc
    34021 atcgctttgg tgaccacata cgggccaacc gctttcaggc cgcgcgggcc gcgcattgcg
    34081 tcagcgccga acacctggaa acgcggggag ccgccgccgg aacctgcaat caggccaacg
    34141 cgcgggttat tctggtaagc ttccggagag aggcccgcat cagcgattgc ctgctccata
    34201 gaaaggaatg cataaatgga tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg
    34261 ccagtggtat ccagttttac gttgccccag acgtggctac gcatgccgga atccttcagc
    34321 tcctgagaga aagtgatccc tgaacgtcct tcacgcagag atgccaggac ttcctgctgg
    34381 ttattaccga tgctggaaac aatgcccagg ccagtaatca ctgcacgttt cattcaatac
    34441 ctctgtaagt cgcacataga gtaagtttcg aatgcacaat agcgtacact tgtacgccga
    34501 acaagtccga tcagccattt aatagagaaa tttgcgcagc cttacacaca tcgctaagat
    34561 cgagccaccg cctgtaagac gagtaactta cgtgaaacac tactccatac aacctgccaa
    34621 cctcgaattt aatgctgagg gtacacctgt ttcccgagat tttgacgatg tctatttttc
    34681 caacgataac gggctggaag agacgcgtta tgtttttctg ggaggcaacc aattagaggt
    34741 acgctttcct gagcatccac atcctctgtt tgtggtagca gagagcggct tcggcaccgg
    34801 attaaacttc ctgacgctat ggcaggcatt tgatcagttt cgcgaagcgc atccgcaagc
    34861 gcaattacaa cgcttacatt tcattagttt tgagaaattt cccctcaccc gtgcggattt
    34921 agccttagcg catcaacact ggccggaact ggctccgtgg gcagaacaac ttcaggcgca
    34981 gtggccaatg cccttgcccg gttgccatcg tttattgctc gatgcaggcc gcgtgacgct
    35041 ggatttatgg tttggcgata ttaacgaact gaccagccaa ctggacgatt cgctaaatca
    35101 aaaagtagat gcctggtttc tggacggctt tgcgccagcg aaaaacccgg atatgtggac
    35161 gcaaaatctg tttaacgcca tggcaaggtt ggcgcgtccg ggcggcacgc tggcgacatt
    35221 tacgtctgcc ggttttgtcc gccgcggttt gcaggacgcc ggattcacga tgcaaaaacg
    35281 taagggcttt gggcgcaaac gggaaatgct ttgcggggtg atggaacaga cattaccgct
    35341 cccctgctcc gcgccgtggt ttaaccgcac gggcagcagc aaacgggaag cggcgattat
    35401 cggcggtggt attgccagcg cgttgttgtc gctggcgcta ttacggcgcg gctggcaggt
    35461 aacgctttat tgcgcggatg aggcccccgc actgggtgct tccggcaatc gccagggggc
    35521 gctgtatccg ttattaagca aacacgatga ggcgctaaac cgctttttct ctaatgcgtt
    35581 tacttttgct cgtcggtttt acgaccaatt acccgttaaa tttgatcatg actggtgcgg
    35641 cgtcacgcag ttaggctggg atgagaaaag ccagcataaa atcgcacaga tgttgtcaat
    35701 ggatttaccc gcagaactgg ctgtagccgt tgaggcaaat gcggttgaac aaattacggg
    35761 cgttgcgaca aattgcagcg gcattactta tcctcaaggt ggttggctgt gcccagcaga
    35821 actgacccgt aatgtgctgg aactggcgca acagcagggt ttgcagattt attatcaata
    35881 tcagttacag aatttatccc gtaaggatga ctgttggttg ttgaattttg caggagatca
    35941 gcaagcaaca cacagcgtag tggtactggc gaacgggcat caaatcagcc gtttcagcca
    36001 aacgtcgact ctcccggtgt attcggttgc cgggcaggtc agccatattc cgacaacgcc
    36061 ggaactggca gagctgaagc aggtgctgtg ctatgacggt tatctcacgc cacaaaatcc
    36121 ggcgaatcaa catcattgta ttggtgccag ttatcatcgc ggcagcgaag atacggcgta
    36181 cagtgaggac gatcagcagc agaatcgcca gcggttgatt gattgtttcc cgcaggcaca
    36241 gtgggcaaaa gaggttgatg tcagtgataa agaggcgcgc tgcggtgtgc gttgtgccac
    36301 ccgcgatcat ctgccaatgg taggcaatgt tcccgattat gaggcaacac tcgtggaata
    36361 tgcgtcgttg gcggagcaga aagatgaggc ggtaagcgcg ccggtttttg acgatctctt
    36421 tatgtttgcg gctttaggtt ctcgcggttt gtgttctgcc ccgctgtgtg ccgagattct
    36481 ggcggcgcag atgagcgacg aaccgattcc gatggatgcc agtacgctgg cggcgttaaa
    36541 cccgaatcgg ttatgggtgc ggaaattgtt gaagggtaaa gcggttaagg cggggtaatc
    36601 tgctctcgca gtgtttgccg gatgcggcgt gaacgcctta tccgacctac gtgtgacgtg
    36661 taggcctgat aagacgcgat aagcgtcgca tcaggcatgg tgctccagac gccgcaactt
    36721 actgctgtga cgcctgctga aacaggtttt cccacatatc ggtcaccagc gcctggtcac
    36781 gcggcgacaa ttcaccggca ccaatggctt tttccagact ctggctaacg gtcgtgtgca
    36841 ccgcctgagc ggagtggtca tcaccacttt ccagttctgc gatggctaat gtcaggtggc
    36901 cacgcaaata cccactggca aacaactcat catcactggc atggtcaacc atatcgtcga
    36961 ttaatgccag aatgcgtgat tcaaactccg cgatcatctt ctttcctcat tgtaaaacgt
    37021 ggcgctggct ttagttgagc gcttccggcc acgggaactg ttccgccgta agttccggcg
    37081 tgtgataata attttgtagt gctttaatga agcgtgccgg gcgttcagga atgccgtttg
    37141 ctaggtaatc catcacctgc gcatgtaccc gccgttgaaa caccacacga tccggttcga
    37201 agtcgccttc cagattgtcg caactgacgt taaacggata tcccgccgcc acacagaaca
    37261 accagtcgaa cgcctgcggc ttcacttcaa catcttcaaa ctggctttgc gtttgggcat
    37321 cgcgtccgtc cgggcaatac cagtagccga aatcaaccag ctcacggcgc gctttcccgg
    37381 caatacacca gtgcgaaatc tcatgaattg cgctggcata aaagccatga gcaaagacga
    37441 ttcggttata cggtacttcc gcatcagcag gaagatagat cggttcgtcg tcgcctttaa
    37501 tcagacgggt attaaattca tcggcaaagc agctattaaa aatttcaatc aactgctcat
    37561 agtggtgtgt actgttcatt agttcatccc caaccagtgg aggatctcct gtccgtggct
    37621 atcaaaaagt aatttggcac tcatcaccgc tgagacaata acaatcatcg ggcggatcag
    37681 cttttgtcct ttgctcaaca ccagtcgtga acccatgcgc gcgccgagga actgccccac
    37741 cagcatgaca aagcccgtcg cccaaatcac tttgccaccg agaataaaca gcagtaaacc
    37801 gccgatgttt gacgttgcgt tgagtaattt ggcgtgcgcc gtggctttgg cgaggttgaa
    37861 tccgcacagc gtaacaaagg ccagtgcgta aaacgatccg gctgccggac caaagaatcc
    37921 gtcataaaaa ccgacgcagc caccggcaat taacgcgaag ggtaaaccgt acatccgacg
    37981 ctggcgatct tcttcaccca gctttggcat cagcaagaaa tagaggccga tacaaattac
    38041 cagaatgggc aaaatctgcc gtaagacatc agcctgaacg tattgcacca gcagtgcgcc
    38101 gctcattgag ccgacaaagg tcatggcgat attgagtttc tgatcgctta aactaaccac
    38161 tttgcggcga ataaagtaga tagtggcgga aatagagccg ccgcaggctt gcagtttatt
    38221 ggttgccagc gcgttagcgg gagacattcc cgcagccatc aatgccggaa tggtgagtaa
    38281 cccaccgcca ccggcaatcg agtcgataaa tcccgccagc atggcgacaa aaaagagaac
    38341 tcccagcaac agcggggaaa ccataaacag gctattaaac gtttccatta gatcacgtgc
    38401 tcatccagta gcgcctggca ggaaggcggc aacggaggcg gtgtcttctt ctcaggcttt
    38461 gttgttcccg gttttggagg tgcaaaccag ctttgcagtt ctgccccgca accatcgcct
    38521 ggtggcggta aaggttgatc ttcacactcc agactatcgg caggacaacg taatcgtaca
    38581 tgcatatgcg cgcgatgctg gaaccagggt cgcactttgc gcaaccagtc gcgatcggtg
    38641 cccgcatcaa ggcaaagttg ttgtttaatc gccggattaa caaaaatgcg tgtgacgtct
    38701 ttgtcctggg cggcgagttt gatcaagctg aaaatttctg gcttccacag cgtggagaca
    38761 acgtgtttac cgtcgcggga aactaagtcc agtgcttgcg ggcgcaagag ctgcgcggag
    38821 gtccagcgag ttttcggcag ttgcagaaag atatcgacat ccagtccggt ctggtggctg
    38881 gcatgaccgc cgttgaaacg cccaccagcg ggcatcccca tatcgccaat cagcaccgta
    38941 cccatgccca gattgctcac ctggctactc agacgctgga taaacatcac cagatccggg
    39001 tgaccgaaat agcgacgctg atcggtacgc atgacctgat aatgttcgga ctgtatcggc
    39061 agcgtgtcag cgccgacgat acagccatta gaaaaactgc ctatcgattg tgcgctaccc
    39121 ggcacaggtt gggttatttt ttgccacggc gtcgctgcca ggctagcgct actggcaagc
    39181 agagccagca gcgcaatcgc ggttttgttc attttttacc agcgtggaat atcagtcttc
    39241 acatcggcat tttgcgcccg ctgccgtaac aggtgatcca ttaaaacgat cgccagcatc
    39301 gcttcagcga tcggcactgc acggatcccg acacagggat cgtgacggcc tttggtgatc
    39361 atctcaactt cttcgccaaa gcggttaata gtgcgccccg gcacggtaat gctggaggtc
    39421 ggtttcagcg ccatatgagc aatgatttgc tgcccgctgc tgataccgcc gagaatgccg
    39481 cccgcatggt tgctctggaa accgtctttg gtgatttcgt cgcggttctg gctgccgcgc
    39541 agcgccacta cgtcaaaacc gtcgccaatt tccacgcctt tcaccgcgtt gatgctcatc
    39601 agcgcatggg cgatgtcggc atccaggcgg tcaaagaccg gctcgccaag tccggcgggg
    39661 acaccactgg caacaacggt gactttcgcg ccgatggagt cgccctcttt tttcagcgcg
    39721 cgcatcagtt catctaacgc gtcgattttg tcggggtccg ggcagaagaa cggattttgt
    39781 tcaaccagcg accagtcttt gatttccagc ggaatgtcgc ccatctgggt caagcagccg
    39841 cggatttcaa taccaaattt ctcggcgaga tattttttgg caatcgcccc tgccgccacg
    39901 cgcatggcgg tttcgcgggc ggaagaacgt ccaccgccgc gataatcgcg cagaccgtat
    39961 ttttgttcgt aggtgtaatc agcatggcct gggcggaaaa cgtctttaat tgcgctgtaa
    40021 tcctgagagc gctggtcagt gttttcgatc aacaagccaa tgctggtgcc agtagtaacg
    40081 ccttcaaaaa caccggagag aattttgacc tgatccggct cgcggcgctg ggtggtatag
    40141 cgcgatgtcc cagggcgacg acggtcgagg tcatgttgca ggtccgcttc cgtcagcgga
    40201 atgcctggcg gaacaccatc gacgatgcag ccgagcgcca gcccatgcga ttcgccgaag
    40261 gtggttacgc gaaagagttg tccaattgtg tttccagcca tcacggctcc gttattgttg
    40321 tgtttgcgtg tttacttaat ctttataaat cgcgaaatat tcacgggccg ccagcagctg
    40381 ctctttggtg agcataaaca caccatcgcc gccgttatca aactccagcc aggtgaacgg
    40441 aacatccgga tattgttcca taagatgtac catgctgttg ccgacttcac aaatcaacac
    40501 gccatcatca gcaaggtaat ctgccgcgtt tccaagaatg cgacgtgtca gtttaaggcc
    40561 gtcggtgcca gatgccaggc ccagttccgg ctcgtgacgg tattcgtttg gcaggtcgga
    40621 catatcttcc gcgtcgacat acggcgggtt agtgacaatc agatcgtatt gcactttcgg
    40681 caaatcacgg aacagatcgg aacgaatcgg aatgacgttg tggatcagac cgtgttcttc
    40741 gatgttctgt tcggcaaccg ccagcgcgtc gggagagata tctaccgcgt cgacttccgc
    40801 ttccgggaag gcataagcac aggcaatggc gatgcagccg ctaccggtac acatatctaa
    40861 aatatgctgc ggttgcttgc tgatgagtcc ggcaaattta ttgttgatca gttcaccaat
    40921 cggcgagcgc ggcaccagca cgcgttcatc gacgtaaaat tcatgaccgc agaaccaggc
    40981 tttgttggtc aggtaagcca ccggaatgcg ctcgttgacg cggcggatca cgcgttcaac
    41041 aatacggtgt ttttcgctgg aggtcagacg cgcggtgcgc atatcttccg ggatatccag
    41101 cggcaggtag agcgatggta acaccagctg tacggcttca tcccacgggt tatcggtgcc
    41161 gtgaccgtac cagatatttg ccgcgctgaa gcggcttacc gaccagcgca acatgtcctg
    41221 aatggtttgc agctcattta ctgcttcatc aacgaaaatt ttatccacgt attcctccag
    41281 ggcatgatcg caataatttc ggcggctagt ttgccatgaa gatgacgata aatcagcatt
    41341 cacgcgcggt gagtgaggaa aaatacgttt aaaacgatcg attgtgctac gagtcgggta
    41401 aactgtagga aaattagaaa tagagacaga taaatgaaaa agaaaacaac acttagcgag
    41461 gaggaccagg ctctgtttcg ccagttgatg gcggggactc gcaagattaa gcaggacacg
    41521 attgtccacc gaccgcagcg taaaaaaatc agcgaagtgc cggtgaaacg cttgatccag
    41581 gagcaggctg atgccagcca ttatttctcc gatgagtttc agccgttatt aaataccgaa
    41641 ggtccggtga aatatgttcg cccggatgtc agccattttg aggcgaagaa actgcgccgc
    41701 ggcgattatt caccggaatt gtttttggat ttacacggtc tgacgcagct gcaggccaag
    41761 caggaactgg gcgcgttgat tgccgcctgt cgccgcgaac atgtgttttg tgcctgcgtg
    41821 atgcatgggc acgggaagca tattttgaag caacaaacac cgctgtggct ggcacaacat
    41881 ccgcatgtga tggcatttca ccaggcaccg aaagagtacg gcggtgatgc ggctttgttg
    41941 gtgttgattg aagtggaaga gtggttgccg ccggagttgc cctgaggagt tgagcgtgtt
    42001 gtgccaggag cgcggaatgg caggggcgaa tgaccctgcc aggtgagaaa aggtgcgcta
    42061 atacatattc agtatgtaag tgaatcggca ggtagaatct atgggctgtc gagatgtcgt
    42121 gtccacgcgt agcttcatat catgcagttg cctggacgta atcggttgcg gctcaggcgt
    42181 caccgtcact ttggcattat cacagccgga agtcgagaag gtcatctgct gtgcaataaa
    42241 acggcagtag gcaccacgct catcaaaatt attcccgtta cacagtttgc cattttccat
    42301 caggaaagtg ggcagtgttt gctcaataac accacctgtt gactgcaacc tgacgacttt
    42361 gttatgtaca acataatttt cattccagga aacatcatac ggtgccaggg gcaccgccgg
    42421 acatgaagaa ctcaccttcg ttgggccaat atagtcagga aaatcatcat catacgtttt
    42481 agcgaaggga tctctactct taacaaacga cgacacaatc cacggacaac catgtaaatt
    42541 cccatcgtaa taaacaaaca tctttaattc attacatgtc catcccgttt tggtctggct
    42601 atcatactgc tctaatccag gacaggctga catggatacc ctgctgtccg aaattttcga
    42661 catagatggt tgagtagaac tatcgccagt ccacgaaact aaatccggct gtgaattggg
    42721 catgaatgta gccttaaata tagcgtaatt aactgaatct ggcgtcgcta taatcagatg
    42781 aaactcaaac tctgtcgcag gtgaactggc accagctggc acatataact caccaacttt
    42841 cgtcgccgcc tggccgcatg tattgaacat taacagcaat aaaagacata aaacatgacg
    42901 aaggattttc attttaaata tactcaaaag ttgctgtcag gctggcggtg aaatcaccga
    42961 tggttacatc acgaccgctt ttcgcctgga tccaggcccg gaaattaata tcattgttgc
    43021 cgttggtaag cgcaaaggtc gccccggtgg tgttgttaat atccacccgc ttgccgtcga
    43081 gcgtttccat ccctatcccc acgccctgcg cggtggaggt gtcatcgact gccagaaaac
    43141 ccggctgacc gctgtcttcg gtgccgctca gcgtgacttt gacgttgtat cccgccggcc
    43201 ctttgcagtc tttcagctta aacacaaccg gcagatgcgc ggactgccca gataccatca
    43261 aatcccggtt gctgacggtg ggaaaatgga tttctgccag gttggcacca tccaccacca
    43321 gcgtgcagga tttactcagc aggttgccgt aaaaatgcag gttgttgtcg actgcctgag
    43381 ctcctgtgct ggcggtcagc agcaggcctg ctaacagcgc ataaccgggt gaataaatgt
    43441 gtgctgtttt cattggtaat ccacccgtaa ggttgcgtaa gcagaaaagg tcgtgtccgt
    43501 cagcaccgca tcagccgctt tgaccggtac cgcctccagc tttggcttct ggctaaaatc
    43561 ggtggcgtta atggaaagcg gctcgttgag cttaaaacgc tgaccgtcat gctgaagctc
    43621 aattccgaag cccggtacat ccgtctcaat agcagagtcg ttgtaggagg tggcagtccc
    43681 cgtccaggag agtgtcatat tccactcggg atcgcggaca tccgagtcgc agctcagttc
    43741 gtagtccact ttctgacgac cataattgcc atcaatgctg tcgataatga gatcgcgaaa
    43801 ttgcacttcg atggtttgcc cgccgctgat gctacaggcg ggcggcgaca tcagcgtacc
    43861 gtggaatgtg atgtcatctt ccgccgccag cacagccccg caaccagcgc acagtaaaag
    43921 gagcaggaat tttttcattg gtaatccacc accatggtca tcgtggcgtt aaactccgac
    43981 ggcgtcagtt gcaccccgct ctgttttacc ggcaccgctt tcagctgagg ctgagtgttg
    44041 acattaaaag gcgtccagtt attttcacca actgcaaaca gggagttgtc ggcggcgttt
    44101 tctatccgca cccccaggcc ggcgatatcc gtgctgagaa ccgtttctcc gttgatcacg
    44161 gcggtattgc ctttgagctg catccgtaag tcatcgctga ctttattacc gcagttgagg
    44221 gtgtatttcg catcctgttg gtaattggta ccatcgatcc tgttgattat cacattgcca
    44281 aatttcacag cgcttccctt cacggagcaa ggcggcggtc catcgaccag gattcgcagc
    44341 gtcagatcca cctgtttgat atcactgcca gcaacagcca aaccgctgat gccccatccg
    44401 gccagcacca gcaaccctgc gcccgctaca aacttgtgac tcataagtcc ctcccggcat
    44461 cagcctttgc ggctctgctc ttcatccacc tggcagctgt tgccggtgca tatgaagtac
    44521 agcggcatcc gcgcgccgta gtcgttgatg taagtcagca ccggcacgct gtccatcttc
    44581 gcattcagcg gtgcggaggt tttcggctcc atcatcagcg gtgtgaatcc cgccggcggg
    44641 ttgccgcctt ttttagcgct ggcgttgctg atgatcacgt agtacggcgt cgggttattg
    44701 acggtgaagt cctggccgtt acggatgagc gtcaccttat gctgccacgg acttttcatg
    44761 ctggtgttct ccagcgcctt cggtcgccag aacagtttga tacgcgtctg cagcgcaatc
    44821 tgcagtgtgt tcggtttatt tgatttcggc ggaatttcgc gcacgttgaa atagaacacg
    44881 ctctcgcggt cggccggcag tttgttgata tccggcagcc cctgcacctt cacctggccg
    44941 ttcatcatcg catcgatgcg ctgcactggc ggaagcaccg caagcggcga gctgattttg
    45001 ttgccctttg catcctctat ccagctctgc gccagataag gcagtgtggg atcgttgttg
    45061 cgcagcgtga cgctgatgga cttgtcgctt tcgttaaaca ccagccgtgt cctgtcgggc
    45121 gtcaccgaag cgtggctgga gaaactgacc gctgtcgcca gcagcgccag tgccgggaca
    45181 acgatttttg cagtgttagt cagattaaac atggatcatt ccttaatcac gttactggtt
    45241 tactgaaatt gaagtcggag gttccgttgg cgtcacccgc tgtgtctgtt cctgaatcgc
    45301 cggtttgacc gccggcgaag tgataggtgc tacattgcct ttatgctggc atggcagtaa
    45361 caagccgtta aacagatcgg caggcagcgg gtccggcagg ttgatatcgc aatgcgcaac
    45421 accactccag aacacactca tgtgttcgcc aggtttcacc ccagccagat aaacattgcc
    45481 gtcatcatcg acaaggccca ctgtctgctc gttatcattt tttacttctg cgccaaacgg
    45541 aggatggctg ccgtcttgta aacgcagcac cgccatcgct ttttgaccac tgatgacggc
    45601 aaatttgcgg tagccaatcg ccccttcagt tagcgtggct tgtaccaccg actgggttgc
    45661 ttcagcgttt tcaggcaatc tgttgaggtc gatatacgcc tgattgcggt aatagttatt
    45721 gacgtcagaa acgacggctt taccaaacat attggtataa acagcagcac cgttaccttc
    45781 taccggaaca tcggcaacgc catcggcatc aatcaacaag cgtgtcccgc ccatattctg
    45841 ggtacggtga agtgcgccac cgtgggcagt aagcgttgcg ccgccctgta acgacaagcc
    45901 cgcagaggtg tattgccctt catggtagtt cgcactgagg tcaacctgcg ccagcgaacc
    45961 atcatggctg taatagccgt caacgctggt gtgtttgtca ctggtgccga cgttcaactg
    46021 atagtgagtc gcgtcatcga cacggctgaa ataaccgacc tgactgctgt ccgtcccact
    46081 gccatagtta ccgttatagc taacggtact gttgtcgccc cacggcatac tgagcgaaat
    46141 gtacatgcct ttgtcggcct ggttgtcata ctcgtaacgg tagccagtca tcgagatgct
    46201 gacattacga atactgccca tattgaaata gtgcgagagc atgatgttgt agtttgtctg
    46261 ctcctcgcga tcccagtagg tatggcgggt atagttgaga taaaccgaaa cacccgcatc
    46321 gcggaagttc tggttataag tggcggtgta catctctttg tcgttgcccg tgcgtaccat
    46381 tccgctgtca ctggcatcca gatactcgct catggtcata aagttctctt ccgagaagcg
    46441 atatccagca aaagtaacgc ggctgttgag ctggtcaaag tctttggaat aactcacacg
    46501 gaaggagtta ccgtccagcg aacctttgcc ataagcggtg tctttatcca gtttggtgtg
    46561 cgagtgagta acatcaaacg cgaccgcgcc gaatgtagac aaatcgcgac cgacgccaag
    46621 cgccgcagac tgatagtttt catctcccag tgcgccgcca tataacgacc agccgttagc
    46681 gatcccccag gaagcttcag caccagaaaa aaattcaccc tcgacatggt gtccccactc
    46741 ttgcggacgg cccatcatga tcttatagcg aacctgacct gggcgagtga ggtatggcat
    46801 cgaggcggtg ctgatgtcat attcctgcac ctggccgttc tgttcttcaa tgcgaatatg
    46861 caacgtaccg gagacagaat caccaagatc ctgaatacga aacggtccag ccggaacctg
    46921 cgtttcgtaa atcacacgcc ccatctggct gacggtcact tttgctgtgg tgtgtgccac
    46981 gccggaaatg tctggcgcgt agccgcgcag attgggaggc aacatttgat cgtcagtact
    47041 gacactgcca ccaacatagt taaaaccatc aaaaatatcg gatctgaggt aatcctcgcc
    47101 cagcgccagt ttggctttta atgatggtaa cgcccgccag gcatagtagc gactccactc
    47161 ccattttttt tgagtttcat cgccgctgaa ttcatcgtca tcatcattac tgcgagtatg
    47221 ttgatagtta gtctgccagt cagcacgcat acgccacggc cccaggttaa ccccgaccgt
    47281 cccgttgccg ctgatctcgt tactatcatc accgccattt tcttcatgcc gggtttgtgc
    47341 gttgatgctg tagtccgcga cgatcccgga gatgccgtca tcccaacgtg aaggcggatc
    47401 ccagtcggga taagtatatt cgaggtaagc ctgtggcagt gaaatgacta atgcggactg
    47461 gcttaaatca gccttaattt ccatgccttc cagttgaccg gatttcaggc atttagcatc
    47521 gtggctccat tgcagatttt tcgccacgtc ttcttttaaa ccaaactgcg ctaccagttc
    47581 cggtgtcaga caagcatagc ttttgctcgc gtcatcttca ccagcatacc agtaaatatc
    47641 gtactcttcc gccaatggct gtttatttag ttgaacctgt aaattgtatt ttccgggctc
    47701 aacatatccc tggctggaaa aacgcttcag atcaattttt gtgtcgcctt ttaattccag
    47761 aaaacgggaa tcaaactgaa tgtcgtcttc agcccagaca ctgctataac tccctgacat
    47821 tgccagcgca atgcaccacg gaagaatccg taatcgaaaa agagaatggt caggcataca
    47881 cgtttctatc cgtttttatt cttgttcggt caaaatccat ttaaatcccc catagcagtg
    47941 tcgctatggg ggtatgcctt acaggtaagt aatctggaag gtggtatttg cttcgaaatt
    48001 acccagatct ggcgcatcag ctgcgccgac cagccaggct ttaaagttca gtgtctgctt
    48061 cgctttgcct ttattggtag aagtgtcttt cactattttc tggtcaatac ccatgccgct
    48121 tttgtaagag gtacccagag agtcaccgat cgccaggcta acattgttaa atgccgcacc
    48181 ggtatcggta ttgaaaatgg tgtaataatt gccgctattt gcagaagaaa cggtaccagt
    48241 aaaggtagtg gtcattgttt cctgagtatc aaatacgcaa tcttgcagac gaatctggaa
    48301 acttttcggc gtggtggtgc cgttattttt cagtttttcg gcaccgatat cacccatatc
    48361 aacttccagt ttgtgatcgt ccggaacgat agaacacact gagtcttcaa tggtgccgta
    48421 aaaacgtgca gtaccgttgt tacccgcagc gattgttgcc gtagaggtga aaacgcccat
    48481 caccattgcc gcagcaatag ctgttttaac aaacttactc attatttgtc cttaaactat
    48541 aaataattaa aaatataaac attacaatac attgattcag tcaataccca atgtttatac
    48601 ccagccattg aatacggcat gcgatccaaa aagaacaggt cgtagaaaat tgtattcttt
    48661 ggtgtggggc cgcatagtga tgttttcacc cccctgatgc aatttaattt tactaaagag
    48721 taaatgtaga tttaattaat atattgacag aagaaaggtt atcttaagga catccttaaa
    48781 aaatacggaa taagtattta gaaaaaatag aaagtatatc aataatgaat atcaacagct
    48841 taaaaaacaa acaatgaaca tataatgcga ttggcattta tccacttttg acaatataat
    48901 atataaacac tatagccaga atttaatcaa gtttcaatta catcctg
//
LOCUS       DAZGAN020000026        43789 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGAN020000026 DAZGAN020000000
VERSION     DAZGAN020000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 43789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.026"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            202..651
                     /gene="alaE"
                     /locus_tag="ACHKYO_RS08435"
                     /old_locus_tag="pgaptmp_001687"
     CDS             202..651
                     /gene="alaE"
                     /locus_tag="ACHKYO_RS08435"
                     /old_locus_tag="pgaptmp_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HGU4428551.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(688..1032)
                     /gene="ygaC"
                     /locus_tag="ACHKYO_RS08440"
                     /old_locus_tag="pgaptmp_001688"
     CDS             complement(688..1032)
                     /gene="ygaC"
                     /locus_tag="ACHKYO_RS08440"
                     /old_locus_tag="pgaptmp_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HGU4428552.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1184..1513
                     /gene="ygaM"
                     /locus_tag="ACHKYO_RS08445"
                     /old_locus_tag="pgaptmp_001689"
     CDS             1184..1513
                     /gene="ygaM"
                     /locus_tag="ACHKYO_RS08445"
                     /old_locus_tag="pgaptmp_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HGU4428553.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            1761..2006
                     /gene="nrdH"
                     /locus_tag="ACHKYO_RS08450"
                     /old_locus_tag="pgaptmp_001690"
     CDS             1761..2006
                     /gene="nrdH"
                     /locus_tag="ACHKYO_RS08450"
                     /old_locus_tag="pgaptmp_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGU4428554.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2003..2413
                     /gene="nrdI"
                     /locus_tag="ACHKYO_RS08455"
                     /old_locus_tag="pgaptmp_001691"
     CDS             2003..2413
                     /gene="nrdI"
                     /locus_tag="ACHKYO_RS08455"
                     /old_locus_tag="pgaptmp_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGU4428555.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2386..4530
                     /gene="nrdE"
                     /locus_tag="ACHKYO_RS08460"
                     /old_locus_tag="pgaptmp_001692"
     CDS             2386..4530
                     /gene="nrdE"
                     /locus_tag="ACHKYO_RS08460"
                     /old_locus_tag="pgaptmp_001692"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU4428556.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYTYTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFTRSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4540..5499
                     /gene="nrdF"
                     /locus_tag="ACHKYO_RS08465"
                     /old_locus_tag="pgaptmp_001693"
     CDS             4540..5499
                     /gene="nrdF"
                     /locus_tag="ACHKYO_RS08465"
                     /old_locus_tag="pgaptmp_001693"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU4428557.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5855..7057
                     /gene="proV"
                     /locus_tag="ACHKYO_RS08470"
                     /old_locus_tag="pgaptmp_001694"
     CDS             5855..7057
                     /gene="proV"
                     /locus_tag="ACHKYO_RS08470"
                     /old_locus_tag="pgaptmp_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HGU4428558.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGNRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7050..8114
                     /gene="proW"
                     /locus_tag="ACHKYO_RS08475"
                     /old_locus_tag="pgaptmp_001695"
     CDS             7050..8114
                     /gene="proW"
                     /locus_tag="ACHKYO_RS08475"
                     /old_locus_tag="pgaptmp_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HGU4428559.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8172..9164
                     /gene="proX"
                     /locus_tag="ACHKYO_RS08480"
                     /old_locus_tag="pgaptmp_001696"
     CDS             8172..9164
                     /gene="proX"
                     /locus_tag="ACHKYO_RS08480"
                     /old_locus_tag="pgaptmp_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HGU4428560.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9455..10639
                     /locus_tag="ACHKYO_RS08485"
                     /old_locus_tag="pgaptmp_001697"
     CDS             9455..10639
                     /locus_tag="ACHKYO_RS08485"
                     /old_locus_tag="pgaptmp_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4428561.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10763..11500
                     /gene="ygaZ"
                     /locus_tag="ACHKYO_RS08490"
                     /old_locus_tag="pgaptmp_001698"
     CDS             10763..11500
                     /gene="ygaZ"
                     /locus_tag="ACHKYO_RS08490"
                     /old_locus_tag="pgaptmp_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HGU4428562.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11490..11825
                     /gene="ygaH"
                     /locus_tag="ACHKYO_RS08495"
                     /old_locus_tag="pgaptmp_001699"
     CDS             11490..11825
                     /gene="ygaH"
                     /locus_tag="ACHKYO_RS08495"
                     /old_locus_tag="pgaptmp_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HGU4428563.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11916..12446
                     /gene="emrR"
                     /locus_tag="ACHKYO_RS08500"
                     /old_locus_tag="pgaptmp_001700"
     CDS             11916..12446
                     /gene="emrR"
                     /locus_tag="ACHKYO_RS08500"
                     /old_locus_tag="pgaptmp_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HGU4428564.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQIEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12573..13745
                     /gene="emrA"
                     /locus_tag="ACHKYO_RS08505"
                     /old_locus_tag="pgaptmp_001701"
     CDS             12573..13745
                     /gene="emrA"
                     /locus_tag="ACHKYO_RS08505"
                     /old_locus_tag="pgaptmp_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HGU4428565.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13762..15300
                     /gene="emrB"
                     /locus_tag="ACHKYO_RS08510"
                     /old_locus_tag="pgaptmp_001702"
     CDS             13762..15300
                     /gene="emrB"
                     /locus_tag="ACHKYO_RS08510"
                     /old_locus_tag="pgaptmp_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HGU4428566.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15364..15879)
                     /gene="luxS"
                     /locus_tag="ACHKYO_RS08515"
                     /old_locus_tag="pgaptmp_001703"
     CDS             complement(15364..15879)
                     /gene="luxS"
                     /locus_tag="ACHKYO_RS08515"
                     /old_locus_tag="pgaptmp_001703"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGU4428567.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPEEKLQELHI"
     gene            complement(16029..17585)
                     /gene="gshA"
                     /locus_tag="ACHKYO_RS08520"
                     /old_locus_tag="pgaptmp_001704"
     CDS             complement(16029..17585)
                     /gene="gshA"
                     /locus_tag="ACHKYO_RS08520"
                     /old_locus_tag="pgaptmp_001704"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HGU4428568.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(17658..18086)
                     /gene="yqaA"
                     /locus_tag="ACHKYO_RS08525"
                     /old_locus_tag="pgaptmp_001705"
     CDS             complement(17658..18086)
                     /gene="yqaA"
                     /locus_tag="ACHKYO_RS08525"
                     /old_locus_tag="pgaptmp_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HGU4428569.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18083..18649)
                     /gene="yqaB"
                     /locus_tag="ACHKYO_RS08530"
                     /old_locus_tag="pgaptmp_001706"
     CDS             complement(18083..18649)
                     /gene="yqaB"
                     /locus_tag="ACHKYO_RS08530"
                     /old_locus_tag="pgaptmp_001706"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HGU4428570.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(18930..19006)
                     /locus_tag="ACHKYO_RS08535"
                     /old_locus_tag="pgaptmp_001707"
     tRNA            complement(18930..19006)
                     /locus_tag="ACHKYO_RS08535"
                     /old_locus_tag="pgaptmp_001707"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18970..18972),aa:Arg,seq:acg)
     gene            complement(19205..19281)
                     /locus_tag="ACHKYO_RS08540"
                     /old_locus_tag="pgaptmp_001708"
     tRNA            complement(19205..19281)
                     /locus_tag="ACHKYO_RS08540"
                     /old_locus_tag="pgaptmp_001708"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19245..19247),aa:Arg,seq:acg)
     gene            complement(19345..19421)
                     /locus_tag="ACHKYO_RS08545"
                     /old_locus_tag="pgaptmp_001709"
     tRNA            complement(19345..19421)
                     /locus_tag="ACHKYO_RS08545"
                     /old_locus_tag="pgaptmp_001709"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19385..19387),aa:Arg,seq:acg)
     gene            complement(19619..19695)
                     /locus_tag="ACHKYO_RS08550"
                     /old_locus_tag="pgaptmp_001710"
     tRNA            complement(19619..19695)
                     /locus_tag="ACHKYO_RS08550"
                     /old_locus_tag="pgaptmp_001710"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19659..19661),aa:Arg,seq:acg)
     gene            complement(19699..19791)
                     /locus_tag="ACHKYO_RS08555"
                     /old_locus_tag="pgaptmp_001711"
     tRNA            complement(19699..19791)
                     /locus_tag="ACHKYO_RS08555"
                     /old_locus_tag="pgaptmp_001711"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19755..19757),aa:Ser,seq:gct)
     gene            complement(20107..20292)
                     /gene="csrA"
                     /locus_tag="ACHKYO_RS08560"
                     /old_locus_tag="pgaptmp_001712"
     CDS             complement(20107..20292)
                     /gene="csrA"
                     /locus_tag="ACHKYO_RS08560"
                     /old_locus_tag="pgaptmp_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HGU4428571.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(20527..23157)
                     /gene="alaS"
                     /locus_tag="ACHKYO_RS08565"
                     /old_locus_tag="pgaptmp_001713"
     CDS             complement(20527..23157)
                     /gene="alaS"
                     /locus_tag="ACHKYO_RS08565"
                     /old_locus_tag="pgaptmp_001713"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGU4428572.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(23285..23785)
                     /gene="recX"
                     /locus_tag="ACHKYO_RS08570"
                     /old_locus_tag="pgaptmp_001714"
     CDS             complement(23285..23785)
                     /gene="recX"
                     /locus_tag="ACHKYO_RS08570"
                     /old_locus_tag="pgaptmp_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGU4428573.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(24028..25089)
                     /gene="recA"
                     /locus_tag="ACHKYO_RS08575"
                     /old_locus_tag="pgaptmp_001715"
     CDS             complement(24028..25089)
                     /gene="recA"
                     /locus_tag="ACHKYO_RS08575"
                     /old_locus_tag="pgaptmp_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGU4428574.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNSETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(25169..25666)
                     /gene="pncC"
                     /locus_tag="ACHKYO_RS08580"
                     /old_locus_tag="pgaptmp_001716"
     CDS             complement(25169..25666)
                     /gene="pncC"
                     /locus_tag="ACHKYO_RS08580"
                     /old_locus_tag="pgaptmp_001716"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HGU4428575.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(25811..26896)
                     /gene="mltB"
                     /locus_tag="ACHKYO_RS08585"
                     /old_locus_tag="pgaptmp_001717"
     CDS             complement(25811..26896)
                     /gene="mltB"
                     /locus_tag="ACHKYO_RS08585"
                     /old_locus_tag="pgaptmp_001717"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HGU4428576.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            27152..27715
                     /gene="srlA"
                     /locus_tag="ACHKYO_RS08590"
                     /old_locus_tag="pgaptmp_001718"
                     /gene_synonym="gutA"
     CDS             27152..27715
                     /gene="srlA"
                     /locus_tag="ACHKYO_RS08590"
                     /old_locus_tag="pgaptmp_001718"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HGU4428577.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            27712..28671
                     /gene="srlE"
                     /locus_tag="ACHKYO_RS08595"
                     /old_locus_tag="pgaptmp_001719"
                     /gene_synonym="gutE"
     CDS             27712..28671
                     /gene="srlE"
                     /locus_tag="ACHKYO_RS08595"
                     /old_locus_tag="pgaptmp_001719"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HGU4428578.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            28682..29053
                     /gene="srlB"
                     /locus_tag="ACHKYO_RS08600"
                     /old_locus_tag="pgaptmp_001720"
                     /gene_synonym="gutB"
     CDS             28682..29053
                     /gene="srlB"
                     /locus_tag="ACHKYO_RS08600"
                     /old_locus_tag="pgaptmp_001720"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HGU4428579.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            29057..29836
                     /gene="srlD"
                     /locus_tag="ACHKYO_RS08605"
                     /old_locus_tag="pgaptmp_001721"
     CDS             29057..29836
                     /gene="srlD"
                     /locus_tag="ACHKYO_RS08605"
                     /old_locus_tag="pgaptmp_001721"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HGU4428580.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            29941..30300
                     /gene="gutM"
                     /locus_tag="ACHKYO_RS08610"
                     /old_locus_tag="pgaptmp_001722"
     CDS             29941..30300
                     /gene="gutM"
                     /locus_tag="ACHKYO_RS08610"
                     /old_locus_tag="pgaptmp_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HGU4428581.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            30367..31140
                     /gene="srlR"
                     /locus_tag="ACHKYO_RS08615"
                     /old_locus_tag="pgaptmp_001723"
                     /gene_synonym="gutR"
     CDS             30367..31140
                     /gene="srlR"
                     /locus_tag="ACHKYO_RS08615"
                     /old_locus_tag="pgaptmp_001723"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HGU4428582.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            31133..32098
                     /gene="gutQ"
                     /locus_tag="ACHKYO_RS08620"
                     /old_locus_tag="pgaptmp_001724"
     CDS             31133..32098
                     /gene="gutQ"
                     /locus_tag="ACHKYO_RS08620"
                     /old_locus_tag="pgaptmp_001724"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HGU4428583.1"
                     /translation="MSEALQNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(32095..33609)
                     /gene="norR"
                     /locus_tag="ACHKYO_RS08625"
                     /old_locus_tag="pgaptmp_001725"
     CDS             complement(32095..33609)
                     /gene="norR"
                     /locus_tag="ACHKYO_RS08625"
                     /old_locus_tag="pgaptmp_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HGU4428584.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPTPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            33796..35235
                     /gene="norV"
                     /locus_tag="ACHKYO_RS08630"
                     /old_locus_tag="pgaptmp_001726"
     CDS             33796..35235
                     /gene="norV"
                     /locus_tag="ACHKYO_RS08630"
                     /old_locus_tag="pgaptmp_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HGU4428585.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            35232..36365
                     /gene="norW"
                     /locus_tag="ACHKYO_RS08635"
                     /old_locus_tag="pgaptmp_001727"
     CDS             35232..36365
                     /gene="norW"
                     /locus_tag="ACHKYO_RS08635"
                     /old_locus_tag="pgaptmp_001727"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HGU4428586.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(36493..38745)
                     /gene="hypF"
                     /locus_tag="ACHKYO_RS08640"
                     /old_locus_tag="pgaptmp_001728"
     CDS             complement(36493..38745)
                     /gene="hypF"
                     /locus_tag="ACHKYO_RS08640"
                     /old_locus_tag="pgaptmp_001728"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HGU4428587.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAATQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(38898..39425)
                     /gene="hydN"
                     /locus_tag="ACHKYO_RS08645"
                     /old_locus_tag="pgaptmp_001729"
     CDS             complement(38898..39425)
                     /gene="hydN"
                     /locus_tag="ACHKYO_RS08645"
                     /old_locus_tag="pgaptmp_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HGU4428588.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(39574..40584)
                     /gene="ascG"
                     /locus_tag="ACHKYO_RS08650"
                     /old_locus_tag="pgaptmp_001730"
     CDS             complement(39574..40584)
                     /gene="ascG"
                     /locus_tag="ACHKYO_RS08650"
                     /old_locus_tag="pgaptmp_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HGU4428589.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            40844..42301
                     /gene="ascF"
                     /locus_tag="ACHKYO_RS08655"
                     /old_locus_tag="pgaptmp_001731"
     CDS             40844..42301
                     /gene="ascF"
                     /locus_tag="ACHKYO_RS08655"
                     /old_locus_tag="pgaptmp_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HGU4428590.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            42310..43734
                     /gene="ascB"
                     /locus_tag="ACHKYO_RS08660"
                     /old_locus_tag="pgaptmp_001732"
     CDS             42310..43734
                     /gene="ascB"
                     /locus_tag="ACHKYO_RS08660"
                     /old_locus_tag="pgaptmp_001732"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HGU4428591.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
BASE COUNT        10310 a        11741 c        11170 g        10568 t
ORIGIN      
        1 tcggcatgta aaaatccaca gtgtacaaaa aacgagccgt tacggaatat tttatctaca
       61 aaaactgact aaataaaaat ttttcactaa ttgaatagtc atagccagcg atatacgcta
      121 tgcgaaaatg cagatggcaa tgagatccac tgctttcatc tccattaaca tcccattacg
      181 cttttattaa ggagcattag catgttctca ccgcagtcac gcttgcgtca tgcagttgca
      241 gatacgttcg cgatggttgt ttactgttct gtcgtgaaca tgtgtattga agttttcctc
      301 tccggaatga gcttcgaaca gtctttttat tccagattgg tagcgattcc ggtgaacatc
      361 ttaattgcat ggccatacgg tatgtaccgc gatctgttta tgcgcgcggc acgcaaagtt
      421 agcccgtcgg gctggataaa aaatctggct gatatcctgg cttatgtgac gttccagtca
      481 ccggtgtatg tggcgatctt gttagtggtg ggcgcagact ggcatcagat tatggcggcg
      541 gtcagttcaa acatcgttgt ttcgatgttg atgggggcgg tttatggcta cttcctcgat
      601 tattgccgcc gactgtttaa agtcagccgt taccagcagg taaaagcctg actcttcttt
      661 tcgcgactgg catcgccagt cgcgtcttta actggcttcg ccaaataaac cattcaaata
      721 acgttcaagc gcaacacgcg aactaaagcc atgcgggata ccgtaatgct cgtgtcgctc
      781 gcctgctaaa tagagcggaa attgctggta atgatcgcaa gttttaatat cggaagcggg
      841 ttcagccagt gttgaactgc gttcttttaa tgttggatga attaccagag cggtacgtcc
      901 cattcgcgct tcgcgattaa catagacata attttcccca cggcggtaac catacgcttt
      961 ctgggttacc acatcgacag taaaaccgac tttttcaagt acgcgcgcca cctcgtctgg
     1021 tcgtaaatac atatttgatc ctcgttatta tcaaccgcgg gcctacctta cctgattgcg
     1081 cattcacaac gctttcagaa aagtccataa agccgcgatg aacctcagtt aagaaaatta
     1141 tggtctacac tgaaaattac atcgaattct aatgggggat catatgttta accgaccgaa
     1201 ccgcaacgac gttgatgatg gcgtgcagga tatccagaat gatgtcaatc aattagctga
     1261 cagcctcgaa tctgtattga agtcctgggg cagcgacgcc aaaggggaag ctgaagccgc
     1321 acgcagtaaa gctcaggcat tgctgaaaga aaccagggcg cgaatgcatg gtcgtactcg
     1381 cgtccagcaa gccgcacgcg atgccgttgg ctgcgcggat tcttttgttc gtgaaagacc
     1441 ctggtgtagc gtgggtacag cagctgcggt aggtattttt atcggcgcac tgttaagcat
     1501 gcgcaaatcg tagtgcaaaa atgataataa atacgcgtct ttgaccccga agcctggctt
     1561 cggggtttct ttttgcctgg tgaatcacaa aaatccccct accccgtcac gctcatatcc
     1621 agggtaattt cgaccactat ttgctatata ttgtgtggtt gaatcttttt tcaactacat
     1681 ctagtatctc tgtatcaaca gagagacaac ccgacgcgta tcatcgcgcc gtatcttcat
     1741 tttaaacgga aatacgaatc atgcgcatta ctatttacac tcgtaacgat tgcgttcagt
     1801 gccacgccac caaacgggcg atggaaaacc ggggctttga ttttgaaatg attaatgtcg
     1861 atcgcgttcc tgaagcagcg gaagcgttgc gtgctcaggg ctttcgtcag ttgccggtag
     1921 tgattgctgg cgatcttagc tggtctggtt tccgtccgga catgattaac cgtctgcatc
     1981 cagcgccaca cgcggccagt gcatgagcca gctcgtctac ttctccagca gctccgaaaa
     2041 cacgcagcgt tttatcgaac gtttaggtct gcccgcggtg cgcatcccgc tcaatgagcg
     2101 ggaacggatt caggtagacg agccttacat cctgatcgtg ccctcttacg gcggcggcgg
     2161 tacggctggc gcggtgccac gacaggtaat tcgcttttta aacgacgagc acaaccgggc
     2221 gttgcttcgc ggcgttattg cttctggtaa tcgcaacttt ggtgaggcgt atggccgcgc
     2281 cggagatgtg attgcccgga aatgcggcgt gccgtggctg taccgttttg aactcatggg
     2341 tacgcaaagc gatatcgaaa acgttcgtaa aggagtaacc gaattttggc aacgacaacc
     2401 gcagaatgcc tgacgcagga aacgatggat taccacgcgc tgaatgcgat gcttaacctc
     2461 tacgatagcg caggtcgcat tcagttcgat aaagaccgcc aggccgttga cgcctttatt
     2521 gcgacgcatg tgcgtccgaa cagtgtgacc ttcagtagcc agcagcagcg cctgaactgg
     2581 ctggtcaacg aaggttacta tgatgaaagc gttcttaatc gctactctcg cgactttgtc
     2641 attacgctgt ttgcccacgc acacaccagc ggttttcgtt tccagacatt cctcggggca
     2701 tggaagtttt acaccagcta tacgttgaag acattcgacg gtaaacgtta tctggaagat
     2761 tttgccgatc gagtcacgat ggtggcgctg acgctggcac aaggcgatgc gacgctggcg
     2821 ttgcaactga ccgatgaaat gctgtcagga cgctttcagc cagccacgcc aacattcctc
     2881 aactgcggta agcagcagcg cggcgaactg gtttcctgtt ttctgctgcg tattgaagac
     2941 aatatggagt cgattggccg ggcggtaaat tccgcactgc aactgtcgaa acgcggcggc
     3001 ggcgtggcat ttttgctgtc gaatctgcgg gaagcgggcg cgccaattaa acgtattgaa
     3061 aatcaatctt ctggcgtaat tccggtgatg aaaatgctgg aagacgcatt ttcctatgcc
     3121 aaccaactcg gcgcgcgtca gggggctggt gcagtctatt tacatgctca tcatcccgat
     3181 attctgcgtt ttctcgacac gaaacgggaa aatgccgacg aaaaaatccg cattaaaaca
     3241 ctgtcgcttg gcgtggtgat cccggatatc actttccatc tggcaaaaga gaatgcgcag
     3301 atggcgctgt tttcgcccta tgacgttgag cgagtttatg gcaagccgtt tgccgatatc
     3361 gccattagcg aacactatga cgaactggtt gccgatgaac gcatccgcaa aaaatacctc
     3421 aacgcccgtg atttcttcca gcgactggca gaaatccagt ttgagtccgg ctatccctac
     3481 atcatgtatg aagacacggt aaaccgtgct aaccctatcg ccgggcgcat aaatatgagt
     3541 aatctctgct cagaaatttt gcaggttaac agcgcctcag agtatgacga gaatctcgac
     3601 tatacctaca caggccatga tatctcctgc aatttaggtt cgttgaatat tgcgcacacc
     3661 atggattccc ccgattttgc ccgcacggta gagactgccg tgcgcggttt aacggcagta
     3721 tcagatatga gtcatatccg cagcgtgccg tccatcgaag ccggaaatgc cgcctcgcac
     3781 gccatcggac tggggcagat gaatttacac ggctatctgg cgcgagaagg catcgcttat
     3841 ggttcgccgg aagcactgga tttcaccaat ctctatttct atgccatcac ctggcacgca
     3901 ctgcgtacct cgatgttgct ggcacgcgaa cgcggtgaaa ccttcgccgg gttcaaacag
     3961 tcacgctatg ccagtggtga atattttagc caatatctgc aagggaactg gcagccgaaa
     4021 acggcgaaag ttggcgaact gtttacccgt agcggtatta cgttacctac ccgtgagatg
     4081 tgggcgcagc tgcgcgacga cgtgatgcgc tacggcattt acaaccagaa tcttcaggcg
     4141 gtgccgccaa ccggttctat ctcttatatc aaccatgcta cgtcgagtat tcatccgatt
     4201 gtggcgaaag tagagatacg caaagagggc aaaacaggac gcgtttacta ccctgccccg
     4261 tttatgacta acgaaaatct ggcgctatat caggacgctt acgaaatcgg cgcagaaaag
     4321 atcatcgaca cctacgcgga agcgactcgc catgtcgatc aggggctgtc gctgacgctg
     4381 tttttccccg ataccgccac cactcgcgat atcaacaaag cgcagattta cgcctggcgc
     4441 aaaggtatca aaacgctcta ttacatccgc ctgcgtcaga tggcgctgga aggcactgaa
     4501 attgaaggct gcgtctcctg tgcactttaa ggaatatcta tgaaactctc acgtatcagc
     4561 gccatcaact ggaacaagat atctgacgat aaagatctgg aggtgtggaa tcgcctgacc
     4621 agcaatttct ggctaccaga aaaggtgccg ctgtcgaacg atattcctgc ctggcagaca
     4681 ttaactgtcg tagaacaaca actgacgatg cgcgttttta ctggcctgac gctgctcgac
     4741 acgctgcaaa atgttatcgg cgcgccttcg ctgatgcccg atgcactcac gcctcatgaa
     4801 gaagcggtat tatcgaatat cagctttatg gaagcggttc atgcccgctc ttacagttcg
     4861 attttctcga cgctgtgtca gaccaaagat gtcgatgccg cctacgcctg gagtgaagaa
     4921 aacgcaccgt tgcagcgaaa agctcagatt attcagcaac attatcgcgg tgatgatccg
     4981 ctgaaaaaga aaatcgccag tgtgtttctt gaatcttttt tgttctattc cggtttctgg
     5041 ctgccgatgt atttttccag ccgcggaaag ctgaccaata ccgcagatct gatccgtctg
     5101 attatccgcg atgaagcagt ccacggttac tacataggct ataaatatca gaaaaacatg
     5161 gaaaagatat ctctgggaca acgtgaagag ttgaagagtt tcgccttcga tttgttgctg
     5221 gaactctacg acaacgagtt gcaatacacc gatgagctgt acgccgaaac cccgtgggct
     5281 gacgatgtga aagcgtttct ctgttacaac gccaataagg ctttgatgaa tctgggctac
     5341 gaaccgttat ttcccgcaga aatggcggaa gtgaatccgg caatcctcgc cgcgctttcg
     5401 ccgaatgccg atgaaaatca cgatttcttt tccggttcag gctcctctta tgtgatgggg
     5461 aaagcggttg aaacagaaga tgaagactgg aatttctgag ggtgttattt tcaaaaatat
     5521 cactacccgc agcagggaaa taattcccgc caaatagctt tttatcacgc aaatattctg
     5581 tagtgatcta cactgatact ctgttgcatt attcgcctga aaccacaata ttcaggcgtt
     5641 ttttcgctat ctttgacaaa aaatatcaac ttttctcgat ttgctctcag cccttatatc
     5701 acgggaaatt ccggcgattt actcgcatca atattcatgc cacatttgcc atcaggggtt
     5761 gcctcagatt ctcagtatgt tagggtagaa aaaagtgact atttccattg ggtaatatat
     5821 cgacataaac aaataacagg aatctttcta ttgcatggca attaaattag aaattaaaaa
     5881 tctttataaa atatttggcg agcatccaca gcgagcgttc aaatatatcg aacaaggact
     5941 ttcaaaagaa caaattctgg aaaaaactgg gctatcgctt ggcgtaaaag acgccagtct
     6001 ggccattgaa gaaggcgaga tatttgtcat catgggatta tccggctcgg gtaaatccac
     6061 aatggtacgc cttctcaatc gcctgattga acccacccgc gggcaagtgc tgattgatgg
     6121 tgtggatatt gccaaaatat ccgacgccga actccgtgag gtgcgcagaa aaaagattgc
     6181 gatggtcttc cagtcctttg ccttaatgcc gcatatgacc gtgctggaca atactgcgtt
     6241 cggtatggaa ttggccggaa ttaatgccga agaacgccgg gaaaaagccc ttgatgcact
     6301 gcgtcaggtc gggctggaaa attatgccca cagctacccg gatgaactct ctggcgggat
     6361 gcgtcaacgt gtgggattag cccgtgcatt agcgattaat ccggatattt tattaatgga
     6421 tgaagccttc tcggcgctcg atccattaat tcgcaccgag atgcaggatg agctggtaaa
     6481 attacaggcg aaacatcagc gcaccattgt ctttatttcc cacgatcttg atgaagccat
     6541 gcgtattggc aaccgaattg ccattatgca aaatggtgaa gtggtacagg tcggcacacc
     6601 ggatgaaatt ctcaataatc cggcgaatga ttatgtccgt accttcttcc gtggcgtcga
     6661 tattagtcag gtattcagtg cgaaagatat tgctcgccgg acaccgaacg gcttaattcg
     6721 taaaacccct ggcttcggcc cacgttcggc gctgaaatta ttgcaggatg aagaccgcga
     6781 atatggctac gttatcgaac gcggtaataa gtttgtcggc gcagtttcca tcgattcgct
     6841 taaaaccgcg ttaacgcagc agcaaggtct tgatgcggcg ctgattgatg cgccgttagc
     6901 agtcgatgca caaacgcctc ttagcgagtt gctctctcat gtcggacagg caccctgtgc
     6961 ggtgcccgtg gtcgacgagg accaacagta tgtcggcatc atttcgaaag gaatgctgct
     7021 gcgcgcttta gatcgtgagg gggtaaataa tggctgatca aaataatccg tgggatacca
     7081 cgccagcggc ggacagtgcc gttcaatccg cagacgcctg gggtacaccg gcgactgcac
     7141 cgactgacgg tggtggtgct gactggctga ccagtacgcc tgcgccaaac gtcgagcatt
     7201 ttaatattct cgatccgttc cataaaacgc tgatcccgct cgacagttgg gtcactgaag
     7261 ggatcgactg ggttgttacc catttccgtc ccgtcttcca gggcgtgcgc gttccggttg
     7321 attacattct caacggtttc cagcaattgc tgctgggtat gcccgcaccg gtggcgatta
     7381 tcgttttcgc tctcatcgcc tggcagattt ccggggtcgg aatgggcgtg gcgacgctgg
     7441 tttcgctgat tgccatcggc gcaatcggtg cctggtcgca ggccatggtt accctggcgc
     7501 tggtgttaac cgccctgctg ttctgtatcg tcattggttt gccgctgggg atctggctgg
     7561 cgagaagtcc gcgagcggcg aaaattattc gtccactgct tgatgccatg cagaccacgc
     7621 cagcgtttgt ttatctggtg ccaatcgtca tgctatttgg tatcggtaac gtgccgggcg
     7681 tggtggtgac gatcatcttt gctctgccgc cgattatccg tctgaccatt ctggggatta
     7741 accaggttcc ggcggatctg attgaagcct cgcgctcatt cggtgccagc ccgcgccaga
     7801 tgctgttcaa agttcagtta ccgctggcga tgccgaccat tatggcgggc gttaaccaga
     7861 cgctgatgct ggccctttct atggtggtca ttgcctcgat gattgccgtc ggcgggctgg
     7921 gtcagatggt acttcgcggt atcggtcgtc tggatatggg gcttgccacc gttggcggcg
     7981 tcgggattgt gatcctcgcc attatcctcg atcgcctgac gcaggccgtt gggcgcgact
     8041 cacgcagtcg cggcaaccgt cgctggtaca ccactggccc tgttggtctg ctgacccgcc
     8101 cattcattaa gtaactctgc acttgcccgg tgacgccggg cattatcacc ctgccaaaaa
     8161 aaggaataac aatgcgacat agcgtacttt ttgcgacagc gtttgccacg cttatctcta
     8221 cacaaacttt tgctgccgat ctgccgggca aaggtattac tgtaaatcca gttcaaagca
     8281 ccatcaccga agaaaccttc cagacgctgc tggtcagccg tgcgctggag aaattaggct
     8341 ataccgtcaa caaacccagc gaagtagatt acaacgttgg ctacacctcg cttgcttccg
     8401 gcgatgcaac cttcaccgcc gtgaactgga cgccactgca tgacaacatg tacgaagctg
     8461 ccggtggcga taagaaattt tatcgtgaag gggtatttgt taacggcgcg gcacagggtt
     8521 acctgatcga taagaaaacc gccgaccagt ataaaatcac caacatcgca caactgaaag
     8581 atccgaagat cgccaaactg ttcgatacca acggcgacgg taaagcggat ttaaccggtt
     8641 gtaaccctgg ctggggctgc gaaggtgcca tcaaccacca gcttgccgcg tatgaactga
     8701 ccaacaccgt gacgcataat caggggaact acgcagcgat gatggccgac accatcagtc
     8761 gctacaaaga gggcaaaccg gtgttttatt acacctggac gccgtactgg gtgagtaatg
     8821 agctgaagcc agggaaagat gtggtctggt tgcaggtgcc gttctccgca ctgccgggcg
     8881 ataaaaatgc cgataccaaa ctgccgaatg gcgcgaatta cggcttcccg gtcagcacca
     8941 tgcatatcgt tgccaacaaa gcctggaccg agaaaaatcc ggcggcagcc aaactgtttg
     9001 ccattatgca gttgccagtg gccgatatta acgcccagaa cgccattatg catgacggca
     9061 aagcctcaga aggcgatatt cagggccacg ttgatggctg gatcaaagcc caccagcagc
     9121 agttcgatgg ctgggtgaat gaggcgctgg cagcgcagaa gtaattttta ttcaggcgga
     9181 taaggcgttt ttgcctcatc cgccgctctg tacacaatgc ctgatgcgac gctgacgcgt
     9241 cttatcatgc ctacaaaata cactcattcg tatggcggat aaggcgtttt cgccgcatcc
     9301 gccgttctgt gcacaatgcc tgatgcgacg ctggcgcgtc ttatcatgcc tacaaaccac
     9361 atcgcacatt tcaacaatct atctttcatc ccatattcat caacatccgc tattattgat
     9421 ttccagctta atcatcacct gatgaacaaa aataatgact aaacctaatc atgagcttag
     9481 cccggcgctg atcgtgctga tgtctatcgc caccggtctg gcggtagcca gtaactatta
     9541 cgcccagcca ttgctcgaca ccatcgcgcg taacttttcc ctttccgcca gttcggcagg
     9601 ctttattgtt accgccgcgc aattgggcta tgcagcaggt ctgctgtttc ttgttcccct
     9661 cggtgatatg tttgaacgcc gccgcctgat tgtctcgatg accttactgg cagcgggcgg
     9721 tatgttaatc actgccagca gtcagtcact ggcgatgatg atcctcggta cggcattaac
     9781 cggtttattc tctgtcgtgg cacaaattct ggttccgctg gcagcgacgc tggcttcacc
     9841 ggacaaacgc ggcaaagtgg ttggcactat tatgagcggt ctgctgttgg ggatcttact
     9901 cgcacggacc gttgccggat tgctggcgaa tctcggcggc tggcgcaccg tcttttgggt
     9961 tgcctcggtg ttaatggcac tgatggcgct ggcattatgg cgtggtctgc cacaaatgaa
    10021 atcagaaacc cacctcaact acccacagtt gttaggttcc gttttcagca tgtttatcag
    10081 cgataagatc ctgcgcaccc gcgcattgct gggctgcctg acctttgcca acttcagcat
    10141 tctctggacc tcaatggcct ttttgcttgc cgctccacct tttaactaca gcgatggtgt
    10201 cattggtctg tttggacttg cgggagctgc cggagcgttg ggcgctcgtc cggcgggcgg
    10261 ttttgccgat aagggtaaat cgcaccacac cacaactttc ggtctgctgc tgctattact
    10321 ttcatggctg gcgatctggt ttggtcacac ttccgtactg gcgctgatta tcggcatcct
    10381 ggtgctggat ctcaccgtac agggcgtgca tatcactaac cagacggtaa tttatcgaat
    10441 acaccctgat gcgcgtaatc gcctgaccgc aggttacatg accagctact ttattggcgg
    10501 tgccgccggt tcgctaattt cagcctcagc ctggcaacat ggcggttggg ctggcgtttg
    10561 tctggctggc gcgacgattg ccctggttaa cttactggtc tggtggcgag gttttcatcg
    10621 tcaggaagcc gcaaattaag caatcagcaa ccgttttcgt ggctttacac ttataagggt
    10681 gttaagaagc ccatcagtct gataaggtta agatattcat tcagtctatt tataatatta
    10741 acaatcgtta agcgtacact ctatggaaag ccctactcca cagcctgctc ctggttcggc
    10801 gaccttcatg gaaggatgca aagacagttt accgattgtt attagttata ttccggtggc
    10861 ctttgcgttc ggtctgaatg cgacccgtct gggattctct cctctcgaaa gcgttttttt
    10921 ctcctgcatc atttatgcag gcgcgagcca gttcgtcatt accgcgatgc tggcagccgg
    10981 gagtagtttg tgggttgctg cactgaccgt catggcaatg gatgttcgcc atgtgttgta
    11041 tggcccgtca ctgcgtagcc gtattattca gcgtctgcaa aaatcgaaaa ccgccctgtg
    11101 ggcatttggc ctgacggatg aggtttttgc cgccgcaacc gcaaaactgg tacgcaataa
    11161 tcgccgctgg agcgagaact ggatgatcgg cattgccttc agttcatggt catcgtgggt
    11221 atttggtacg gtaatagggg cattctccgg cagcggcttg ctgcaaggtt atcccgccgt
    11281 tgaagctgca ttaggtttta tgcttccggc actctttatg agtttcctgc tcgcctcttt
    11341 ccagcgcaaa caatctcttt gcgttaccgc agcgttagtt ggtgcccttg caggcgtaac
    11401 gctattttct attcccgtcg ccattctggc aggcattgtc tgtggctgcc tcactgcgtt
    11461 aatccaggca ttctggcaag gagcgcccga tgagctatga ggttctgctg cttgggttac
    11521 tggttggcgt ggcgaattat tgcttccgct atttgccgct gcgcctgcgt gtgggtaatg
    11581 cccgcccaac caaacgtggc gcggtaggta ttttgctcga caccattggc atcgcctcga
    11641 tatgcgctct gctggttgtc tctaccgcac cagaagtgat gcacgataca cgccgtttcg
    11701 tgcccacgct ggtcggcttc gcggtactgg gtgccagttt ctataaaaca cgcagcatta
    11761 tcatcccaac actgcttagt gcgctggcct atgggctcgc ctggaaagtg atggcgatta
    11821 tataacccac aagaatcatt tttctaaaac aatacattta ctttatttgt cactgtcgtt
    11881 actatatcgg ctgaaattaa tgaggtcata cccaaatgga tagttcgttt acgcccattg
    11941 aacaaatgct aaaatttcgc gccagccgcc acgaagattt tccttatcag gagatccttc
    12001 tgactcgtct ttgcatgcac atgcaaagca agctgctgga gaaccgcaat aaaatgctga
    12061 aggctcaggg gattaacgag acgttgttta tggcgttgat tacgctggag tctcaggaaa
    12121 accacagtat tcagccttct gaattaagtt gtgctcttgg atcatcccgt accaacgcga
    12181 cgcgtattgc cgatgaactg gaaaaacgcg gttggatcga acgtcgtgaa agcgataacg
    12241 atcgccgctg cctgcatctg caattaacgg aaaaaggtca cgagtttttg cgcgaggttt
    12301 taccaccgca gcataactgc ctgcatcaac tctggtccgc gctcagcaca acagaaaaag
    12361 atcagatcga gcaaatcacc cgcaaattgc tctcccgtct cgaccagatg gaacaagacg
    12421 gtgtggttct cgaagcgatg agctaacgcg tcatctcgct caaaaatcca gatttataaa
    12481 agaaaaaatg accggccagc atcgcaacat gctggccttt ttggcaagca ggtcggctca
    12541 gccgatgagt taagaagatc gtggagaaca atatgagcgc aaatgcggag actcaaaccc
    12601 cgcagcaacc ggtaaagaag agcggcaaac gtaagcgtct gctcctcctt ctcaccttgc
    12661 tctttataat tattgccgta gcgataggga tttattggtt tttggtactg cgtcatttcg
    12721 aagaaaccga tgacgcatac gtggcaggga atcaaattca aattatgtct caggtgtctg
    12781 gcagcgtgac gaaagtctgg gccgataaca ccgattttgt aaaagaaggc gacgtgctgg
    12841 tcactctcga cccgacagat gctcgccagg cgtttgaaaa agccaaaact gcactggctt
    12901 ccagcgttcg ccaaacccac cagctgatga ttaacagcaa gcagttgcag gcgaatattg
    12961 aggtgcagaa aatcgccctc gcgaaagcac aaagcgacta caaccgccgt gtgccgctgg
    13021 gcaatgccaa cctgattggt cgcgaagagc tgcaacacgc ccgcgacgcc gtcaccagtg
    13081 cccaggcgca actggacgtc gcgattcaac aatacaatgc caatcaggcg atgattctgg
    13141 ggactaaact ggaagatcag ccagccgtgc aacaggctgc caccgaagta cgtaacgcct
    13201 ggctggcgct ggagcgtact cgtattgtca gtccgatgac cggttatgtc tcccgccgcg
    13261 cggtacagcc tggggcgcaa attagcccaa cgacgccgct gatggcggtc gttccagcca
    13321 ccaatatgtg ggtggatgcc aactttaaag agacgcagat tgccaatatg cgtatcggtc
    13381 agccggtcac tatcaccacg gatatttacg gcgatgatgt gaaatacacc ggtaaagtgg
    13441 ttggtctgga tatgggcaca ggtagcgcgt tctcactgct tccagcgcaa aatgcgaccg
    13501 gtaactggat caaagtcgtt cagcgtctgc ctgtgcgtat cgaactggac cagaaacagc
    13561 tggagcaata tccgctgcgt atcggtttgt ccacgctggt gagcgtcaat accactaacc
    13621 gtgacggtca ggtactggca aataaagtac gttccactcc ggtagcggta agcaccgcgc
    13681 gtgaaatcag cctggcacct gtcaataaac tgatcgacga tatcgtaaaa gctaacgctg
    13741 gctaatccag aggtgcgtgt gatgcaacag caaaaaccgc tggaaggcgc gcaactggtc
    13801 attatgacga ttgcgctgtc actggcgaca ttcatgcagg tgctggactc caccattgct
    13861 aacgtggcga tccccactat cgccgggaat ctgggctcat cgctcagcca gggaacgtgg
    13921 gtaatcactt ctttcggggt ggcgaatgcc atctcgatcc cgcttaccgg ctggctggca
    13981 aagcgcgtcg gggaagtgaa actgttcctt tggtccacca tcgcctttgc tattgcgtcg
    14041 tgggcgtgtg gtgtctccag cagcctgaat atgctgatct tcttccgcgt gattcagggg
    14101 attgtcgccg ggccgttgat cccgctttcg caaagtctat tgctgaataa ctacccgcca
    14161 gccaaacgct cgatcgcgct ggcgttgtgg tcgatgacgg tgattgtcgc gccaatttgc
    14221 ggcccgatcc tcggcggtta tatcagcgat aattaccact ggggctggat attcttcatc
    14281 aacgtgccga ttggcgtggc ggtggtgttg atgacactgc aaactctgcg cggacgtgaa
    14341 acccgcaccg aacggcggcg gattgatgcc gtggggctgg cactgctggt tattggtatc
    14401 ggcagcctgc agattatgct cgaccgcggt aaagagctgg actggttttc atcacaggaa
    14461 attatcatcc ttaccgtggt ggcggtggtg gctatctgct tcctgattgt ctgggagctg
    14521 accgacgata acccgatagt cgatctgtcg ttgtttaagt cgcgcaactt caccatcggc
    14581 tgcttgtgta tcagcctcgc gtatatgctc tacttcggcg ctattgttct gctgccgcag
    14641 ttgttgcagg aggtctacgg ttacacggcg acctgggcag gtttggcctc tgcgccggta
    14701 gggattattc cggtgatcct gtcgccgatt atcggccgct tcgcgcataa actggatatg
    14761 cggcggctgg taaccttcag ctttattatg tatgccgtct gcttctactg gcgtgcctat
    14821 acctttgaac caggtatgga ttttggcgcg tcggcctggc cgcagtttat ccaggggttt
    14881 gcggtggcct gcttctttat gccgctgacc accattacgc tgtctggttt gccaccggaa
    14941 cgactggcgg cggcatcgag cctctccaac tttacgcgta cgctggcggg gtctatcggc
    15001 acgtccatca ccaccaccat gtggaccaac cgcgagtcga tgcaccacgc acagttaacc
    15061 gagtcggtaa acccgtttaa cccaaacgcc caggcgatgt acagtcaact ggaagggctt
    15121 gggatgacgc aacagcaggc ctccggctgg attgcccagc agatcaccaa ccaggggctg
    15181 attatttccg ccaatgagat cttctggatg tcagccggga tattcctcgt cctgctgggg
    15241 ctggtgtggt ttgctaaacc gccatttggc gcaggtggcg gcggaggcgg tgcgcactaa
    15301 gtacaactaa gccagttcat ttgaactggc ttttttcaat taattgtgaa gatagtttac
    15361 tgactaaatg tgcagttcct gcaacttctc ttccggcagc gccagttctt cgttgctgtt
    15421 gatgcgcacg tcacgttcca gaatgctacg cgcaatatcc tgcgcttcct gcaacgagtg
    15481 catctggtaa gtgccacact ggtagacgtt cagttccggg atctgattct gatcctgcac
    15541 tttcagcacg tcttccattg ccgctttcca ggcatcagca acacgctgct catctggcgt
    15601 accaatcaga ctcatataaa aaccggtgcg gcagcccatt ggcgagatat cgataatctc
    15661 tacaccatta ccgttaagat ggttacgcat aaaaccagca aacaggtgct ccagggtatg
    15721 gatccctctt tctggcatca cttctttgtt cggcacgcag aagcgcagat cgaacacggt
    15781 gattgcgtcg ccatgcgggg tgttcattgt tttcgccacc cgaactgcag gcgcttccat
    15841 ccgggtatga tcgactgtga agctatctaa caacggcatt tatccacctc cggtaatttt
    15901 tttaaaaatt ttctgaactc tttcttccca ggcgagtctg agtatatgaa agacgcgcat
    15961 ttgttatcat catccctgaa ttcagagatg aaattttggc cactcacgag tggccttttt
    16021 cttttctgtc aggcgtgttt ttccagccac accgcaaacg gttcggtatc tgcggcttcc
    16081 atttcctgct gacggcgttc agatgcctcg cgctcggcta caaaatcctc ttcgcgcaga
    16141 atttccagcg gctcttcacg cagcagatta cggtacgctt cagcaaacgc tttgcctgtt
    16201 ccgccaatac cagtatcaat catagacctt aagatacggg cagagaaagt cagatcggga
    16261 ttatcgaagc aggcaaccag ttcatcacac actttctgat acgcttcgcc gccgttaatg
    16321 ctatccagcg tttgcgcgac gcgtttcaga tcgcggaaca gatctttacc cacctgcagt
    16381 aacgggaact gcgcggtttc gcagccgata cccagcgtca ggcccggttt gcgaccttca
    16441 aggatcaccc ggttccagtt aacgcgcgta caggcaagtt cgctactgct catttccggt
    16501 gcatcagcca gcgcacacca gaccataaac aggtcgagga atcgcacctg ctgttcatct
    16561 acaccaatcg gcgagaacgg gttgatgtcc agcgaacgca cttcaatata ttcaatgccg
    16621 ccacgtaaca gcgcatcaga aggtgactcg ccgctgcggg taacgcgttt tggacgaatc
    16681 ggcgcataca gttcgttttc aatctgcagc acgttgctat tgatttgcag cctcttaccg
    16741 tctttctcaa taccaatctt cgcgtactct tccgatggcg ttttgattgc ctgtttaagg
    16801 cccgctacgt actcgtaaag atcgttgaag gtaataccaa gattgctttg cgatttattg
    16861 gtatagccga gatcgctcaa acgaagagag gtcgcatacg gcaggtaata cataccgcac
    16921 tcggttttct caaacggcag cgacgttggt tttccttgca ggaaagaaga acaaatcgcc
    16981 ggagatgcac caaacagata aggaatgacc caaccgaaac gatagtaatt gcggataacg
    17041 cggaaatagc ccgcagaaat tttctctttg gcatcagcgc ccgagatatc accgcacttc
    17101 gcttgccaga atgccattgg caaagagaaa ttgtagtgca cgccggaaat ggtttgcatc
    17161 agcgcgccgt agcgattttt cagcccttca cgatacagcg ttttaaagcg tccggtgtta
    17221 gaagtgccgt actgtgccag ttcgatgtcc tgaccttctg cgatgtagca tggcatactt
    17281 aacggccaca tccgctcatc gcccatattg cgcgccgtat aacgatgcag atcgcgcata
    17341 aaggtcaaca tatgttcaat atcaccatcc actggtgtaa tgaattccag caatgcttcc
    17401 gcaaaatcgg tagtaatcca tttgtgcgtc agtgcggaac ctaatgcttc aggatgacct
    17461 gttgttgcca gtgtgccatc agcattaaca cgcaaagttt cgcgctccag cccacgctgt
    17521 atccccttta acgcctgagg atgtttttcc agccaggcca gcgcctgtga tacgtccggg
    17581 atcaaattga cctcccgcct gtcaaaatcg ttttaattag cataactgta atggtgacca
    17641 tatgtgcagg cctacaatta gtgccaccac atcatgccct gaacggtagc tgctgcaact
    17701 gcaacatagc gtaacgcttt accaaggcac aaaaaaaaga ttaccggtcc ccacgagatg
    17761 cgcatccatc ccgctaacag acacagtaaa tcgccaacca cgggcatcca gcttaataat
    17821 agcgtgactg caccatagcg tttcagccag ccagtagctt tctcttgcca gcgcgatgtt
    17881 ttacgcaatg gaaagaaacg cccaaggata acgttagtta accctccaag gctattaccc
    17941 attgttgctg ttaaaactaa aacccaggga taactgatcc cggaaagcaa cattgccacc
    18001 agcacgactt cggagttgcc gggtaatagt gtagcgctga gaaaactact ggcaaacaac
    18061 gagaaaagcg ataacgcttc actcacagca agcgaacatc cacggcgtcc atgcctgctg
    18121 cacgggccgc ctgaataccg aaatcggcat cttcaaagac cacacactgc gtcggttgca
    18181 cgcccatacg ctgcgcgcac aacaaaaatg tgtctggcgc gggtttatgg tgtttgacgt
    18241 gatcagcagc gacgacggcg tcaaaataac ggcgtaatcc caggtgcgcc agcaatgcct
    18301 cagcgatggc gctttcactc cccgttccta cagccattgg gcgacgacca tgccaacttt
    18361 tcaccacttc aacaagagga agcggctcga cactatccag cagcatactt cttactgctt
    18421 ctgttttttc acgcgctaac gcatgcgggt cgagatcggc ctgattcagc tcaataattg
    18481 cctgagcaat acgccaggtg ggcgatccat taagcgcaat cattgcctga acatcgtact
    18541 gaagaccgta gtgccctaat acttcgcgcc acgctttacg gtgcgtaggc tccgtatcca
    18601 ggattgtgcc atccatatca aaaattaaac ctgcataacg ctcgtacatg gtcttctcgc
    18661 aagtcgaaaa atatgacgct actttagcgt aattgcttga ttttgtcgct gatggcgaag
    18721 gagaagaaaa ttcaggaaat ggataaagta gttatttaga aatgagatat ttttgaagga
    18781 aatttttggg agaagatggt gcatccggga ggattactca ctggcgttcg cccttcgggc
    18841 cgttgctgac gcaacgttat cctccctggt gcctgcggtt aataccgcta tctcttcgat
    18901 accttcattg ctgaaggtta cggagaatat ggtgcatccg ggaggattcg aacctccgac
    18961 cgctcggttc gtagccgagt actctatcca gctgagctac ggatgcatcg ggaaaattta
    19021 ttttactgct gatattgata tcgctactaa agccatatca agtaagagat ggtgcatccg
    19081 ggaggattac tcgccttgcg gctcgccctt cgggccgttg ctaaagcaac gttatcatcc
    19141 ctggtgcctg cggttaattc cgctacctct tcgatacctt cattgctgaa gatttcggag
    19201 aatatggtgc atccgggagg attcgaacct ccgaccgctc ggttcgtagc cgagtactct
    19261 atccagctga gctacggatg catcgggaaa atttatttta ctgctgatat tgataccgct
    19321 actaaagcca tatcaagtaa gagatggtgc atccgggagg attcgaacct ccgaccgctc
    19381 ggttcgtagc cgagtactct atccagctga gctacggatg catcgggaaa cttactttac
    19441 tgcagatttt ttgataccgc tactaaagcc gtatcaagta agagatggtg catccgggag
    19501 gattactcgc cttgcggctc gcccttcggg ccgttgctaa agcaacgtta tcctccctgg
    19561 tgcctgcggt tataccgcca cctcttccat accttcatcg ctgaaagtac gtagaatatg
    19621 gtgcatccgg gaggattcga acctccgacc gctcggttcg tagccgagta ctctatccag
    19681 ctgagctacg gatgcaaatg gcggtgaggc ggggattcga accccggatg cagcttttga
    19741 ccgcatactc ccttagcagg ggagcgcctt cagcctctcg gccacctcac cacacgcctc
    19801 ttacgagtgc ttcgaagaac ttgtttatcg ctcatcgtcg ctgcgtggcg cacatattac
    19861 tttctgagac ttataagtca aacaattttt cctacacttt tatcgtttgc acacttcacg
    19921 ttcaattagt ctgtaaaaac gtcaaaaaga gtgttttatc aacagaaaaa tggaggtctg
    19981 acagatagta gtaatgcaaa aaaatggaga cttaagttga atgaacggga gtaaagcgaa
    20041 aagactatag agtgaaggag aaattttgag ggtgcgtctc accgataaag atgagacgcg
    20101 gaaagattag taactggact gctgggattt ttcagcctgg atacgctggt agatctcttc
    20161 acggtgaaca gaaacttcct tcggggcatt tacgccaata cgtacctggt tgccctttac
    20221 ccctaaaact gtcacggtga cctcatcccc aatcatgagg gtctcaccaa ctcgacgagt
    20281 cagaatcagc attctttgct ccttgaaaga ttaaaagagt cgggtctctc tgtatcccgg
    20341 cattatccat catataacgc caaaaagtaa gcaatgacaa acacattaca tgtaagcagt
    20401 catggcatta cattctgtta aacctaagtt tagccgatat acacaacttc aacctgactt
    20461 tatcgttgtc gatagcgtta atgcgaatgc cgtgaagcga gtccacggca ttgcctgacg
    20521 cttatattat tgcaatttcg cgctgaccca gcctttcaca ctggctaacg ctgcaggtaa
    20581 ggccgcagca tccgtaccac cggcttgcgc catgtcagga cgtccaccac ccttgccgcc
    20641 cacctgctga gcgaccatac caatcagttc ccctgctttc acacgatctg tgacgtcctt
    20701 agatacgcct gcaatcagag aaaccttacc ttcggctacc gttgccagca cgataattgt
    20761 cgaccccagc tgatttttta aatcgtcaac catggtacgc aacattttcg gctcaacacc
    20821 gctaagctcg ctaaccaaca gcttaacacc attaacatca attgccttac tggaaagatt
    20881 tgcgctctcc tgtgcggcag cttgttcttt aagctgttgt agttcttttt ctaactgacg
    20941 cgtacgttcc agtactgagc gcactttatc cgccagatta ttgctatcgc ctttcagcag
    21001 atgcgcgact tcgcttaagc gatcgctgtc tgcatgaacg gtggtgatag cgccttctcc
    21061 ggttaccgct tcgatacgac gaacgcctgc agcagtaccc gattcagaga tgatgcggaa
    21121 cagaccaata tcaccagtgc ggctggcgtg agtaccgcca cacagctcgg tggagaaatc
    21181 gcccatgctc agcacgcgta cacgctcatc atacttctcg ccgaacagcg ccatcgcacc
    21241 tttcgctttc gccgcttcga gatccatgat gttggtttcg atcggcaaat tgcgacgaat
    21301 ctgtgcgttc accaggtctt cgaccgcacg aatctcttcc ggtttcatcg cttcgttgtg
    21361 tgagaagtcg aagcgcagca ccttgtcgtt aaccagtgaa cctttctgcg atacatgagt
    21421 ccccagaacc tggcgcagcg cagcgtgcat caggtgcgtt gcggagtgat tcagacgaat
    21481 acgggcgcga cgagcctcat caacatcagc ctgcacagcg tcgcccactt tcagagaacc
    21541 cgcagcaagt ttaccgatgt gaccaatcgc ctggccgtat ttctgcgtat cttccaccgc
    21601 gaaggagaag ttagcgcctt tcagttcgcc tttatcacca acctgaccgc cggattccgc
    21661 atagaatggt gtttgatcca gcacgaccac agcttcctgg cctgcattga tggcgtcaac
    21721 cgctttacca tcaacaaaca gcgcggtcac tttgccgttc agttccagat ggtcatagcc
    21781 tttaaattca gatgcactat caacacggat cattgcgttg taatcggcac caaagccgct
    21841 ggcttcgcgc gcgcgacgac gctgctcttc cattgctgct tcaaaaccag cttcgtcaac
    21901 tttgatgttg cgctcacgac aaacatcagc cgtcaggtca accgggaagc cataggtgtc
    21961 gtacagacgg aaagccgttt caccatccag cgtatcacca gaaagttttg ccagctcttc
    22021 atccagcaac gccagaccgc gctccagagt acgggcaaac tgctcttctt cagtcttcag
    22081 cacctgctca acctgcgcct gctggcgttt caggtcttca cccgcagagc ccataacgtc
    22141 gatcagcgga ccaaccagtt tgtagaagaa ggtttctttc gcgccgagca tattgccgtg
    22201 acgcactgcg cgacgaatga tacgacgcag tacataacca cggttttcat tggacggcat
    22261 tacgccatcc gcgatcagga acgcacaaga acgaatgtgg tcagcgatta cgcgcagcga
    22321 tttattgctc agatcggtcg cgccagtgac tttcgctacc gcctggatca acgtgcggaa
    22381 caggtcgatg tcatagttag agttaacgtg ttgcagcacc gcagcaatac gctccagacc
    22441 cataccggta tctacagacg gcttcggcag cggttccatc gtgccatctg cctggcggtt
    22501 gaactgcatg aagacgatgt tccagatctc aatgtagcgg tcgccgtctt cttccggact
    22561 tcccggaggg ccaccccaaa tgtggtcgcc gtgatcgtag aagatttcgg tgcacgggcc
    22621 gcacggacca gtgtcaccca tctgccagaa gttgtcagat gcgtatggcg cacctttgtt
    22681 atcgccgatg cgaataatac gttcgcgcgg gatcccgact tctttttccc agatttcgta
    22741 ggcttcgtcg tcgctttcat agacggtaac ccacagacgc tctttcggca gggcaaacca
    22801 tttttcgctg gtcagcagtt cccatgcaaa ctgaatggca tcgtgtttga aatagtcgcc
    22861 gaagctgaag ttgcccagca tttcgaagaa ggtatggtga cgcgcggtgt aaccgacgtt
    22921 ttccaggtcg ttgtgtttac cacccgcacg cacgcagcgt tgggaagtgg tagcgcggga
    22981 ataattacgc ttgtcgagcc caaggaacac atccttgaac tggttcatcc cggcgttggt
    23041 aaacaacaaa gttgggtcgt tatggggtac cagggagctg ctggcaacta cctgatgtcc
    23101 cttactatgg aaaaagtcga gaaacgcctg acggatctca gcggtgctct tgctcataat
    23161 tatcctgaaa tcaagctaac gaaatatcgc caccagctcc agcgtgtctt aaccgccggg
    23221 ctggtaactg aaaagtggga ataagataag ttttcttgac tgggaagtaa aataccgtat
    23281 gcgttcagtc ggcaaaattt cgccaaatat cctggatatc ttccatcaga tagccacgat
    23341 agagcagaaa acgctggatc ttaacttttt ctgaaaagac agttggcaaa ggttcaccat
    23401 attttcgcgt cgcctgatcg cgcgccagtg cgcaccagtc gatgtcacat tcacgcatcg
    23461 ctttttctgt cgcttcgcgg gaaatacctt tctgattcag ttcctgacga atacgtgcag
    23521 gtccataacc tttgcggcta cggctggcga taaagcgcgc aacaaatcgg ctgtcatcga
    23581 gatagccatg ttcatggcac caggcaataa cgcgctcgta atcttctgcc gtagcatcaa
    23641 tctcttctgg gccatttttg cccataatcg gtgccgcgag tttacgtcgc agttcttgct
    23701 cactgtgatc gcgcaccgcc agaatgcgta ccgcacgatc caacaggcga gcatatgccg
    23761 ggcgacggga cgttgattct gtcatggcat atccttacaa cttaaaaaag caaaagggcc
    23821 gcagatgcga cccaggtttt gctgataaac agatttaata gtcaatgagt gtgcagaatg
    23881 ttcccgcagg cccaattcgt tagaggatcg aggtgagagt aatttttcac gccagtgctg
    23941 gcctgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtc cgtgcacaca
    24001 ttatctatta tcatcaataa cgaacgatta aaaatcttcg ttagtttctg ctacgccttc
    24061 gctgtcatct acagagaaat ccggcgttga gttcgggttg ctcagcagca actcacgtac
    24121 tttcttctcg atctctttcg cggtttccga gttatctttc agccaggcag ttgcattcgc
    24181 tttaccctga ccgatcttct cacctttgta gctgtaccac gcgcctgctt tctcgatcag
    24241 cttctctttt acgcccaggt caaccagttc gccgtagaag ttgatacctt cgccgtagag
    24301 gatctgaaat tcagcctgtt taaacggtgc agcgattttg ttcttcacca ctttcacgcg
    24361 ggtttcgcta cccaccacgt tttcgccctc tttcaccgcg ccgatacgac ggatgtcgag
    24421 acgaacagag gcgtagaatt tcagcgcgtt accaccggta gtggtttccg ggttaccgaa
    24481 catcacacca attttcatac ggatctggtt gatgaagatc agcagcgtgt tggactgctt
    24541 caggttaccc gccagcttac gcatcgcctg gctcatcata cgtgccgcaa ggcccatgtg
    24601 agagtcgccg atttcgcctt cgatttccgc tttcggcgtc agtgccgcca cggagtcaac
    24661 gacgataacg tctactgcgc cagaacgcgc cagggcgtca cagatttcca gtgcctgctc
    24721 gccggtgtcc ggctgggagc acagcaggtt atcgatatcg acgcccagtt tacgtgcgta
    24781 gattgggtcc agcgcgtgtt cagcatcgat aaacgcacag gttttacctt cacgctgcgc
    24841 tgcggcgatc acctgcaacg tcagcgtggt tttaccggaa gattccggtc cgtagatttc
    24901 gacgatacgg cccatcggta gaccacctgc cccaagcgcg atatccagtg aaagcgaacc
    24961 ggtagagatg gtttccacat ccatggaacg gtcttcaccc aggcgcatga tggagccttt
    25021 accaaattgt ttctcaatct ggcccagtgc tgccgccaac gctttctgtt tgttttcgtc
    25081 gatagccatt tttactcctg tcatgccggg taataccgga tagtcaatat gttctgttga
    25141 agcaattata ctgtatgctc atacagtatc aagtgttttg tagaaattgt tgccacaagg
    25201 tctgcaatgc atacgcagta gcctgacgac gcaccgcatc acggtcgccg ctgaagcatt
    25261 cccgccgggt aatgccttca ccgcgggcag tggcaaaagc aaaccagacg gtgccgacag
    25321 gcttctcttc actgccgcca tccggcccgg cgataccact aatagacacg gcataatcag
    25381 cacgagccgc tttcagtgcg cctatcacca tttccaccac gacgggttca ctcaccgcgc
    25441 catgctgcgc cagcgtctct tcgcgtacgc cgatcatctg cgctttggct tcgttactgt
    25501 aggtgacaaa tccgcgttca aaccaggcgg agctaccggc aatatcggta atcactttcg
    25561 ctacccaacc accggtacaa gactcggcag ttgttacggt tgcgccacgg gctttcagcg
    25621 cctgcccaac ctgttcactt aactgcatca gttcactgtc agtcatcaca acctctgtca
    25681 gtcaaaaatt tatgccggac aagatagcac tttcgtcagg aagatggtgt tggcgttgca
    25741 attttacgag gaggattcag aaaaaagctg attagccaga gggaagctca cgcccccctc
    25801 ttgcaaatag ttactgcacg cgcgccagcg ccacggcttg tcctaactgc cagaccgcca
    25861 ttgcgtaatg ggtgctgtgg ttataacggg taatggtgta gaagttcggc agaccgtacc
    25921 agtactggta accggtaccg acatccagac gcagcaggct ggcttgttga tggttgccca
    25981 gcggctgctg tggcgttaaa cctgccgtgg caagctgcga aatgctgtac ctggttttga
    26041 agccatttgg caagcctgga gcctgaccgt ttgccattac cgcgacctga tcgcctttca
    26101 cccagccgtg cgctttgaaa tagttcgcca cgctaccgat cgcatcaacc ggatcccaca
    26161 ggttgatatg cccgtcgccg ctgaaatcta ccgcatattg tttgtaagac gacggcataa
    26221 actgtccgta gcccatcgcc ccggcaaagg aacctttcag attgagcgga tcgtcctgct
    26281 cgtcgcgcgc catcaacagg aaggtttcca gctcgccaga gaaatactcc gcgcggcgtg
    26341 ggtagttaaa tgacagcgtt gccagcgcat cgaggatgcg agttttcccc atcacgcgcc
    26401 cccagcgggt ttcaacgccg ataatcccga cgataatttc cggcggtacg ccatacacct
    26461 gccacgcgcg attcaacgca tcttcatact gattccagaa aaccacaccg ttctgcacgt
    26521 tgtccggcgt aataaatttt ttgcgataac ggagccatgc gccgttcgga cctgatggcg
    26581 gtttcaccga tgtggttggt gcctggttat ccatcagccg cagtaccgaa tccagacgtt
    26641 tcgcctggga gagaatttcc tgcaactgct gacgatcgaa accgtgtttg ttcaccattt
    26701 tgtcgatgaa ctgctgggca ttcgggttat tagcgaaatc gccgcccatc tgcatcacat
    26761 tgtgctgcgg ctcaagcagg aagccgccag acggcgttcc ggtggtcgtt tcagtctcag
    26821 taggttttgg cttgctgcta caggcggcaa gcaacacaaa aaggggaagc aatgttacat
    26881 aacgacgctt gaacatgagg ggtccattta acagattcaa ccaggggcaa gtatggtaaa
    26941 gcatcacgcc ccacacaagg aagcggtagt cactgcccga tacggacttt acataactca
    27001 actcattccc ctcgctatcc ttttactcaa actttcaaat taaaatattt atctttcatt
    27061 ttgagatcaa aataacactt ttaaatcttt caatctgatt agattaggtt gccgtttggt
    27121 aataaaacaa taaatcctga aggagagaac aatgatagaa accattactc atggtgcaga
    27181 gtggtttatc gggctgttcc aaaagggcgg agaggtgttt accgggatgg tgaccggcat
    27241 tcttccgctg ttgattagcc tgctggttat catgaacgca ctgattaatt ttatcggtca
    27301 gcatcgtatt gaacgttttg ctcaacgttg cgccggtaac cctgtttccc gttacctact
    27361 gttaccgtgc attggcacgt ttgtcttttg caatccgatg accttaagcc tgggtcgctt
    27421 tatgccggaa aagtacaaac ccagctacta cgcggcggcc tcttatagct gccactcaat
    27481 gaatggcctc ttcccccata tcaaccctgg cgaactgttt gtttatcttg gcattgccag
    27541 cggtctgaca acgctgaacc tgccacttgg cccactggcg gtgagttatc tgctggttgg
    27601 tctggtcacc aatttcttcc gcggctgggt gaccgatctg accaccgcca tttttgagaa
    27661 aaagatgggc attcaacttg aacaaaaagt tcacctagca ggagcaacat catgacgcat
    27721 attcggatcg aaaaaggaac gggtggctgg ggcggcccgc ttgagctgaa agccacaccg
    27781 ggaaaaaaaa tcgtctatat caccgccggt acccggcctg cgattgttga caaactggca
    27841 cagcttactg gctggcaggc tattgacgga tttaaagaag gtgaacccgc ggaggcggaa
    27901 attggtgtcg cggtaatcga ctgtggcggc acattacgct gcggcatcta tccgaaacga
    27961 cgtattccca ccattaatat ccactcgacg ggcaagtccg gtccgctggc gcagtacatt
    28021 gtggaagata tttatgtctc tggcgtaaaa gaagaaaaca tcactgtagt gggtgatgcg
    28081 acaccacaac cctcttccgt gggccgtgac tatgacacca gtaagaaaat caccgaacaa
    28141 agcgatggtt tactggcgaa ggtgggaatg ggcatggggt ccgccgttgc ggtgctgttt
    28201 caatctggtc gtgacaccat cgacactgta ttaaaaacca ttctgccgtt tatggcattc
    28261 gtctcggcgc tcattggcat cattatggct tctggccttg gtgactggat tgcccacggt
    28321 cttgctccgc tggcgagcca tccactgggt ctggtcatgc tggcgctcat ctgctccttc
    28381 ccactgcttt cacctttcct cggcccaggc gcagttatcg cacaggttat cggcgtattg
    28441 attggcgtgc agattggtct cggcaatatt ccgccgcatc tggctttacc tgcactgttt
    28501 gccatcaacg cgcaggcggc ctgcgacttc atcccggtcg gtttgtcgct ggcggaagcc
    28561 cgtcaggaca cggttcgcgt cggtgtccct tctgtactgg tgagccgctt tttaaccggc
    28621 gcgccgactg tactgatcgc ctggtttgtc tccggtttta tctatcaata gaggctgaaa
    28681 catgaccgtt atttatcaga ccaccatcac ccgtatcggc gcgagtgcca ctgacgccct
    28741 cagcgaccag atgctcatca cctttcgtga aggcgcgcca gcggacctcg aagagtattg
    28801 cttcattcat tgccacggcg agttgaaagg tgcactccat cccggtttgc aattttcact
    28861 cgggcagcat cgctatccgg tgaccgctgt tggcagcgtg gcggaagaca accttcgcga
    28921 actgggtcat gtcaccctgc gcttcgatgg tttaaacgaa gcggaatttc cgggcactgt
    28981 ccatgtggca ggccctgtac ccgacgatat cgcgccggga tcggttttga agtttgaatc
    29041 tgttaaggag taaaaaatga atcaggttgc cgttgtcatc ggtggtgggc aaaccttagg
    29101 cgcgttcctg tgccacggtc tggctgccga ggggtatcgc gtcgcggttg tcgatattca
    29161 gagcgacaaa gccgcaaatg tggcacaaga aattaacgcc gaatatggtg aaggtatggc
    29221 gtacggtttt ggtgctgacg ccaccagcga gcaaagcgtt ctggcgctct ctcgtggggt
    29281 agatgaaatc tttggtcgcg tggatttgct ggtctatagc gccggaatag caaaagcagc
    29341 ctttatcagc gacttccagc tcggcgattt tgaccgttcg ctacaagtga atctggtggg
    29401 ttatttcctg tgtgcccgtg aattttcgcg tttgatgatc cgcgacggga ttcagggtcg
    29461 cattattcag atcaactcga aatccggcaa agtgggtagc aaacacaact ctggctacag
    29521 cgcagcgaaa tttggtggcg tcgggctgac tcaatcactg gcgctggatc tggcggagta
    29581 cggcattacg gtgcattcac tgatgctcgg taacctgctg aaatcgccga tgttccagtc
    29641 actgttgcca caatacgcga ccaagctggg tatcaaaccg gatcaagtcg agcagtatta
    29701 catcgacaaa gtaccgctca aacgcggctg cgattatcaa gatgtgctga atatgctgct
    29761 gttctacgcc agtcctaagg cgtcgtactg caccggacag tcgatcaatg tcaccggcgg
    29821 tcaggtgatg ttctgatcaa cagcggagat ccgttaagga tctccgtgag actatagaat
    29881 gcctgatgcg ctacgctcat caggcataca ggacttccgc cactacatta aggaaaagtt
    29941 atggtatccg cactcatcac cgtcgccgtt atcgcctggt gtgcgcaact ggccttaggc
    30001 ggctggcaaa tttctcgttt taaccgtgcc ttcgacacac tatgccagca agggcgggtt
    30061 ggcgtgggcc gttccagcgg gcgctttaaa ccgcgggtcg tggtcgccat cgccctggac
    30121 gatcagcagc gcatcgtcga caccttgttt atgaaaggac tgaccgtctt cgcccgaccg
    30181 caaaaaattc ccgcaattac cggtatgcat gcgggtgatt tacagcccga tgtgatcttt
    30241 ccccatgatc cactatcaca gaatgctcta tcattggcgc ttaaactgaa acgtggataa
    30301 tttcgttgtg aatgttactt gcttgcgaag ttatcatttt gaaacctaaa tcaggtaatc
    30361 acgcccatga aacctcgtca gcgtcaggcc gccattctgg agtatctgca aaagcagggt
    30421 aaatgctcgg ttgaagaatt ggcgcaatac tttgacacca caggcacaac cattcgcaaa
    30481 gatctggtca ttctggaaca tgccggaacc gtcattcgta cttatggcgg agtggtgttg
    30541 aataaagagg aatccgatcc gcctatcgat cataaaacac tcatcaacac ccacaagaaa
    30601 gagctgattg cagaagctgc cgttagtttt atccatgatg gcgattcgat cattcttgat
    30661 gctggcagta ccgttttgca gatggttccc ctgctctcgc gctttaataa catcacggtg
    30721 atgaccaaca gcctgcatat cgtcaatgcg ctatccgaac tggataacga acaaactatc
    30781 ctgatgccag gcggaacgtt tcgtaaaaaa tcggcctcat ttcacggaca gctggcagag
    30841 aatgccttcg aacatttcag tttcgataaa ttgtttatgg gcaccgacgg catcgatctc
    30901 aatgcgggcg taaccacctt caacgaggtt tataccgtca gtaaggcaat gtgcaatgcc
    30961 gcgcgcgaag tgattttgat ggcggactca tcaaagtttg gccgtaaaag ccccaacgta
    31021 gtttgcagtc ttgaaagcgt cgataagctg attaccgacg caggtatcga tccggcgttt
    31081 cgtcaggcgc tggaagagaa agggatcgat gtgatcataa ccggagagag caatgagtga
    31141 agcactacag aacgcgggac gtcagacgtt aatgctggag ttgcaggaag caagccgttt
    31201 accggaacgt ctgggcgatg attttgttcg cgccgccaat atcatcctgc actgcgaagg
    31261 caaagtggtg gtttcgggaa ttggcaaatc gggccacatt ggtaagaaaa tcgccgcaac
    31321 acttgccagt accggcacac cggctttttt tgtccatccg gcagaagcac tgcacggcga
    31381 tctggggatg attgaaagcc gcgatgtgat gctgtttatc tcttactccg gtggcgcgaa
    31441 ggaactggat ctgattattc cgcgtctgga agataaatct atcgcgctgc tggcgatgac
    31501 cggcaaaccg acgtcaccgc tgggcctggc ggcaaaagcg gtgctggata tctccgtaga
    31561 acgcgaagcc tgcccgatgc accttgcgcc gacctccagc accgtcaata ccctgatgat
    31621 gggtgacgcg ctggcgatgg cggtcatgca ggcgcgcgga tttaatgaag aagattttgc
    31681 ccgctcccac ccagccgggg cactgggcgc tcgcttgctg aataaagtgc atcatctgat
    31741 gcgccgtgac gatgccatcc cacaggtggc gttaaccgcc agcgtgatgg atgcgatgct
    31801 ggaactcagc cgcaccggtc tggggctggt ggcggtatgt gacgctcaac aacaggtaca
    31861 aggcgtcttt accgacggcg atttacgtcg ctggctggtt ggcggcggcg cactcaccac
    31921 gccagttaat gaagcgatga cggtcggcgg caccacgttg caatcgcaaa gtcgcgccat
    31981 cgacgccaaa gagatcctga tgaagcgcaa aatcactgcc gcaccggtgg tagatgaaaa
    32041 cggcaaactc accggcgcaa ttaacctgca ggatttctat caggccggga ttatttaatc
    32101 cttcattccc agacgtttcg ccagccgatg caggttggcg acgtcggttt ccagcatccg
    32161 cgcgcaggca gcccagttgt gatgattttg tgccagcgcc tgacgaatag tttcacgctg
    32221 gaacgcttct gtcgcttcac gcaggttttg cttaacaacg ggcaccgccg ccacttctgg
    32281 cgtcggcaac gtcacctcag gaaaagcaaa atgttgcgcc tcaagaatca cttcatcgcc
    32341 gttgcgggtg gctctcgaca gcactaccgc ccgatgaata gcatgttcca gttcgcgcac
    32401 gttccccgga aaacgatagt gttgcagtaa atttcgcgct ccggcactta ataccacgcg
    32461 ggagagcccc agccgcaaac gacactgctc gcagaaatac cccgccagca gaatgacatc
    32521 atcgccccgc tcacgcagcg gcggcaccga aagtggaaac acgctcaggc gatgaaacaa
    32581 gtcagcgcga aatcgccctg ccagcacctc ttcgcgtaag tcgcggttag tcgccgccag
    32641 cacgcgcaca tcgacccgca aactgcggtc atcgccaacg cgctgaatat cgccatactg
    32701 caacaccctc agcagcttgg cctgcaatgc caacgacaac tcgccgatct catccagaaa
    32761 cagagtgccg ttatccgcca tttcaaactt cccgctgcgg ttactgatag cgccagtaaa
    32821 cgctcctttc acatgcccga acagttcact ttccgccaca ctttccggca gtgcggcaca
    32881 gttgagatag accagcggat tcaccgcccg tggcgaggct tcatgaatcg ctttcgccac
    32941 cagctcctta ccggttcccg tctcaccgct gatcaggacg ttgagatcgg acgccgccac
    33001 aatctcaatc tcttttttca gttgcgtcat gccaggggac aagccaatca tctgcgtctg
    33061 tttcaccgct tcaaacggcg tggcatcgcc tggcatcata ttctggcttt ccagttgttc
    33121 aatcagcaac gcattgctta acgctcccgc cgccagcgca gcaatcagcc gtagctcttc
    33181 gtcgctgaaa acatcgaact gatcgggctg catcccgtcg agcgtcagtg cgccgatcag
    33241 gttttgcccg gcaaacaatg gcagaccaac gcaggcgtga accttcagac tctcctgccc
    33301 aggaatcaaa ccgtcatagg gatcgggcaa ttcgctgtct gcgggaaagc gcaccacatc
    33361 cccggcgcgg gcaatcgctt ccagccgtgg atgcccttcc agcgcaaagc gtctaccgag
    33421 tacatccttt gccagaccgt cgatggcaag cggaataaac tgccgcgaat cgtaacgtag
    33481 caacgcagac gcatcgcact ccagcacctg acgtagcgtg gtgatcaggc gctgaaaacg
    33541 atcctgatga ccaatcccac gctgcaattc gatggcgata ttcgccagca catcaacgga
    33601 aaaactcatc tttgcctcac tgtcaatttg actatagata ttgtcatatc gaccatttga
    33661 ttgatagtca ttttgactac tcattaatga gcatactttt atttatatag taaaaacaat
    33721 cagataaaaa actggcacgc aatctgcaat tagcaagaca tctttttaga acacgctgaa
    33781 taaattgagg ttgctatgtc tattgtggtg aaaaataaca ttcattgggt tggtcaacgt
    33841 gactgggaag tgcgtgattt tcacggcacg gaatataaaa cgctgcgcgg cagcagttac
    33901 aacagctacc tcatccgcga agaaaaaaac gtgctgatcg acaccgtcga ccataaattc
    33961 agccgcgaat ttgtgcagaa cctgcgtaat gaaatcgatc tggcggatat cgattacatc
    34021 gtgattaacc atgcagaaga ggaccacgct ggggcgctga ccgaactgat ggcgcaaatt
    34081 cccgatacgc cgatctactg tactgccaac gctatcgact cgataaatgg tcatcaccat
    34141 catccggagt ggaattttaa tgtggtgaaa actggcgaca cgctggatat cggcaacggc
    34201 aaacagctca tttttgtcga aacaccaatg ctgcactggc cggacagcat gatgacttac
    34261 ctgacaggcg acgcggtgct gttcagtaac gatgctttcg gtcagcacta ctgcgacgag
    34321 catctgttca acgatgaagt ggatcagacg gagcttttcg agcagtgcca gcgttactac
    34381 gccaatatcc tgacgccgtt cagccgcctg gtaacgccga aaattaccga gatcctgggc
    34441 tttaacttac cagtcgatat gatagcgacc tcccacggcg tggtctggcg cgataacccg
    34501 acgcaaattg tcgagctgta cctgaagtgg gccgctgatt atcaggaaga cagaatcacc
    34561 attttctacg acaccatgtc gaataacacc cgcatgatgg ctgacgctat cgcccagggg
    34621 attgcggaaa ccgacccacg cgtggcggtg aaaattttca acgtcgcccg aagcgataaa
    34681 aacgaaatcc tgaccaatgt cttccgctca aaaggcgtgc tggtcggcac ttcgacgatg
    34741 aataacgtga tgatgccgaa aatcgccggg ctggtggagg agatgactgg tttacgcttc
    34801 cgtaacaaac gcgccagtgc tttcggctct cacggctgga gcggcggtgc ggtggatcgt
    34861 ctttccacgc gcctgcagga tgcgggtttc gaaatgtcgc ttagcctgaa agccaaatgg
    34921 cgaccagacc aggacgctct ggagttatgt cgtgaacacg gtcgcgaaat cgcccgtcag
    34981 tgggcgctcg cgccgctgcc gcagagcacg gtgaatacgg tggttaaaga agaaacctct
    35041 gccaccacga cggctgacct cggcccacgg atgcagtgca gcgtctgcca gtggatttac
    35101 gatccggcaa aaggcgagcc aatgcaggac gttgcgccag gaacgccgtg gagtgaagtc
    35161 ccggataact tcctctgccc ggaatgctcc ctcggcaaag acgtctttga agaactggca
    35221 tcggaggcaa aatgagtaac ggcattgtga tcatcggttc gggcttcgcc gcccgccaac
    35281 tggtgaaaaa tattcgcaaa caggacgcca ctattccatt aaccctgatt gccgccgaca
    35341 gcatggatga gtacaacaaa cctgacctca gccatgttat cagtcagggg caacgtgccg
    35401 atgaccttac ccgccagacg gcgggtgaat ttgccgagca gtttaatctg cacctgtttc
    35461 cacaaacctg ggtgacggat atcgatgccg aagcccgtgt ggtgaaaagc cagaataatc
    35521 agtggcaata cgacaagcta gtactggcaa ccggtgccag tgcctttgtc ccgcctgtgc
    35581 ctgggcgtga gttaatgctg acgttaaata gtcagcaaga gtatcgcgcc tgtgaaacgc
    35641 aactgcggga tgcccgacgc gtgttgattg ttggcggtgg tttgattggt agcgaactgg
    35701 cgatggattt ttgtcgtgca ggcaaagcgg tcacgctaat cgacaacgct gccagtattc
    35761 tggcgtcgtt aatgccaccg gaagtaagca gccgcttgca gcatcggttg acggagatgg
    35821 gcgttcatct gttgttgaaa tctcagttgc aggggctgga aaaaacagat tctggcattc
    35881 tggcaacgct ggaccgccag cgcagtatcg aagtggatgc ggtaattgcc gccaccggac
    35941 tgcgcccgga aaccgccctg gcacgacgcg ccgggctgac gattaatcgc ggcgtttgcg
    36001 tcgatagtta tctgcaaacc agtaatgccg atatttacgc gctgggcgat tgcgcggaaa
    36061 ttaacggtca ggtattgccg ttcctccagc cgattcaact tagcgcgatg gtgctggcaa
    36121 aaaatcttct cggcaataac acgccactga aactaccggc gatgctggtg aaaatcaaaa
    36181 cgccggaatt accgctgcat ctggcaggcg aaacccagcg tcaggattta cgctggcaaa
    36241 ttaataccga acgccaggga atggtggctc gcggtgttga cgatgctgac cagcttcgcg
    36301 cctttgtggt cagtgaggat cggatgaaag aggcatttgg attgttgaaa acattgccga
    36361 tgtaggtggg ctactgtgcc taaaatgtcg gatgcgacgc tggcgcgtct tatccgacct
    36421 acggggacgc atgtgtaggc cggataaggc gtttacgccg catccggcaa tggtgtccaa
    36481 atgcaacacg ttttatccgt tctggacttc acccgctaac caacgcgccg cagcaataac
    36541 cccctgcccc agagacaaac cgccatcacc cgccggtaaa ctctgtggaa agagcaatgt
    36601 gaaatcagcg agataatgcg ccagacgtgc acgcagcaaa cggttatgaa taaccccgcc
    36661 gctaaatacc agcgtagtga taccacgcat cgtggcctgc tcacgcatca acgcggcaaa
    36721 accctgcgcc agcgcatcat gaaacgccca cgcgcgttga ttaaccggtg cctgccagtt
    36781 cagccactgc tgccagaaag tggcgagatc cagttgattg tccaccagcg gcattgtcac
    36841 cggatgcgtc actccgtggc acgaagctgc gagtgcctcc agagcacaag ccgcttcacc
    36901 ttcataactt aacgtggctg gcgcacagcc cagtgccgcc gccacggcat cgaacaaccg
    36961 cccacacgat gacgccagcg gcgcgttaat tccacgctca atggcccgcg ccagcacgct
    37021 ccagttttgc tgttgcacac ttgctgtttc agaataattc tgccactccg gcacaaagcg
    37081 caggcactgc gccagcaggt ttcgccacgg ctgcgtcgcc gccaaatcgc cacccggaag
    37141 cgccactgca ggcaagccgc ccaggtgctc acattcgcga tagttcaccc gcagacactc
    37201 gccgccccac aaagcgccgt tctcccccat accgatacca tcgagcgtca aagcaatgac
    37261 atcaccgcca tccagcggcc actgatgctc tgccagacac gccgctgcgt gggcatgatg
    37321 atgcagcacc gtttgcgtcg gcatattcat ttcgctcgcc cactggctgg agacatagcc
    37381 cggatgcacg tcatgcacaa cgtattgcgg agtgaaatcg tagatgtttt gcatcaggcg
    37441 taacgcttcg cgccactgca tctggatgcc atcgtcactt aaatcgccca gatgctgact
    37501 caacaccgct tgttcaccgc gcaccaggca gaaggtgttt ttcagatccg cgccaagaca
    37561 cagcacaggc ggaacatttt taaagcccgg aggcaaaacc agcgcatccg gcacataccc
    37621 gcgtgaacgg cgcagcattt cgccgctttc gcgcaccacc gaatcatcca tccgctgcac
    37681 gatgtcgcgg ttatgtatca agaatccgtc ggcaatgccc tgcaaatccg ccagcgcctg
    37741 ttcgttgctg atagctggtg gtttaccgct caggttgccg gaggtcatca ccagcgggca
    37801 ttgcagttcc tgtaacagca aatgctggag cgggttcgca ggcaacatta ccccgacttc
    37861 gttaaggtca ggggcgatat catcacaaag ctcaggaacg tattttttat ccaccagcac
    37921 aatcggcgcg gcgggcgtgg taagcaactg gcgcgcagcg tctggtaaac cgtcagccac
    37981 tggcaacatg accgccagcg gtttcgccgg gcgatgtttg cgcgcccgca gtgtcgccac
    38041 cgcgttactg ttacgtgcat cgcaggcaag atgaaatccg ccaatccctt tgatggcgac
    38101 aatgttgccc atttttaact gtgcgatagc cgcctgtaat gccgcctctt gttctgcatg
    38161 ttcaccatga cttacccatt caagatgtgg gccacactcc gggcaggcca ccggctgggc
    38221 gtggaagcga cgatcgagcg ggtcgcggta ctctttgtca caagccggac acaacggaaa
    38281 cgccgccatc acggtaaacg ggcggtcgta aggcatggcg cgaataatgg tgaaacgcgg
    38341 gccgcagtgg gtacagttga taaacggata acgataacgc cgttcgcctg gggtattcat
    38401 ttcggcaaga caggcagggc aagtagcggc atcgggaaca atttgcgtat tcatggtgcc
    38461 gcctgtgctc tggcgtatag tgaactcggt gggcagttgt gaccagatgt acggctcacg
    38521 ctcgacgcta tcaatacgcg ccagcggcgg gcagtgctga tgcaattgaa caagaaacgt
    38581 ttccgggtct tccagcagcc ggacttctac gccatcgccg tcattacaga catcgccgtg
    38641 aagatttaat tgctgtgcca gctgccagac aaacggacga aaaccgacgc cctgcacttt
    38701 gcctcgaata cgcagttgga caccgcaaga tgtgtttttt gccattgagt tattcccgcc
    38761 atcatgaatt gcgtaacccg ccctgccgga cacgactgcg tcgtatccgg cagtctcagg
    38821 tcggcgatac cgccagctcc gtattctacg aatatttccg ggaattcctt tgatgccaga
    38881 acagttctat aagattttta gaacatcagc gccgtacggc gacgtttttc tgcgctcagt
    38941 tgttcaagtt tattacgatc gacacaaatc agcgcatggg tcgggcaagc cgccatacac
    39001 gccgggccgt cttcgcgatg gttgcacagg tcgcatttat tggcttcagc tttgtcagcc
    39061 cgtacattca gccccgcgcc gctgttgcgg atcaccggac gtaccaccac ttccatcgcg
    39121 ccatacgggc aagccaccac gcaggttttg caaccaatgc aacgttcctg catcacatga
    39181 acaaaccctt tatcacggct gatagcacca ttcgggcaga cgttagcgca cggtgcatct
    39241 tcacactgac ggcaaactgt cgccgtggaa atgttcacac ctttaatgac atggatacgc
    39301 ggtaaaaatg tttccggggt cagcgatgca cagtcctgat tttcctgatg agaaaccacg
    39361 cacgccactt cacaggtacg gcaaccaata catttactcg cgtcagcaat gatgaaacgg
    39421 ttcatcaaat tctccagcaa tgacagttaa tgcgccgata cattcacaaa tcatgccagt
    39481 ttttaattca ctgttattta aggaaattaa tttctgtaat gcaggaaaaa agatgtcatc
    39541 gacactagtg acgatgacat gtgatgacaa tgtttatcgc gaaagagcaa tgagtgagtc
    39601 gcggcggatc agttttccgc tgaaggtttt cggcggtgag aaatccccgc catcgagcat
    39661 aaaaatcagc cgtccaataa tttcctgaat catctctgtt accggaattt ttacgctgga
    39721 gagcgccgga acggtgtagg gggcaattgc gatatcatcg aatccgataa ccgacacctg
    39781 ctctggcacc gctacgccgc gctcgtgcaa cgctttgatc gcacctatcg ccatatcgtc
    39841 gttactggca actaacgcgc taaatttagc cccacgttcg agcaacgttt ctaccccttc
    39901 ggccccgctg gcgggcgtcc atttaccgtt agcgataagt ttttcattga gcgcaatacc
    39961 atgctgcgac agcgcgtctt tatacccggc aagacgttca acgctggtgg gggaatccat
    40021 cgagccggta aggaaagcaa tctcctgatg tccggcgttt atcaactctg ccacggcatt
    40081 aaagctggtc tgtttatgat cgcaccagac gctatggctg ctgtttttgc gcaggcggcg
    40141 attaagcacc attatcggct gactgtgcgc gtcaatgatg tcatcgatct catccacgct
    40201 taaaaaacgc gggtaaatca taatggcgtc gcagcgcaaa tccagcagat actgaatcgc
    40261 ctggcgctct tcttcggcgc tgtgtttacc atctgccaat agcaactgcc gccctttctc
    40321 ttccgccatt cgcgcggcat gaaagagtaa ttcactaaaa taaatgccgt ggtaaagcgt
    40381 gttggtcact accagcccca gcgtctgagt actcttcgcc gacagattgc gcgccagcaa
    40441 gtttgggcgg taaccgctct cttctaccgc ctgaaacacg cgatctttag tctcctggct
    40501 gacgtagcca ttacctgaaa gcacgcggga aacggtcgct tttgaaaccc cggcgcgctt
    40561 cgccacttcc agcatcgtcg tcatcatttt catcccttta cacgcaatca acgcagtgta
    40621 ctgcaccgtt tgccgattgt ccttgcgcaa tcggcgggaa aaatattcag gtgaccggtt
    40681 tcgcaaatat aaaaaatgaa caattaactc tcttgcttat ttagtgacaa ctattcatga
    40741 ttttgtgaaa ccggtttcct aattccgttt cagcatcggc atttttccgt tacgtcgact
    40801 gataacaact acatctaccc tactgataac aggataaaat ccgatggcca aaaattatgc
    40861 ggcgctggca cgctcggtga tagcggcact gggcggcgtt gataacatct cggcagtcac
    40921 gcactgtatg acgcgtttgc gctttgttat caaagatgac cagcttatcg acagcccgac
    40981 gttaaaaacc atccccggcg tgctcggcgt ggtacgtagt gacaaccagt gtcaggtgat
    41041 tatcggcaat accgtttcac aagcctttca ggaagtcgtc agcctgctgc cgggagatat
    41101 gcagcccgca cagcccgtgg gtaaacccaa actcacgcta cgtcgcattg gtgcggggat
    41161 cctcgatgcg ctgatcggca ccatgtcacc gctgatcccg gcgattatcg gcggatcgat
    41221 ggtcaaactg ctggcgatga tcctcgagat gagcggcgtg ctgacaaaag gatcgccgac
    41281 cttaaccatt ctgaatgtga ttggtgacgg tgctttcttc ttcctgccgc tgatggtcgc
    41341 ggcttcggcc gccatcaaat ttaaaaccaa tatgtcgctg gcgattgcca tcgcgggtgt
    41401 gctggtacat ccgagtttta ttgaactgat ggcgaaagcg gcccagggtg aacatgtcga
    41461 atttgccctg ataccggtca ccgcggtgaa atacacctac acggtgatcc cggcgctggt
    41521 gatgacctgg tgcctgtcat atatcgaacg ctgggtggat cgcattacac cggcggtgac
    41581 caaaaacttc ctcaagccaa tgctgattgt gttgattgcc gcaccgctgg caatcctgct
    41641 gattggcccg attggtatct ggatcggtag cgccatttcg gcgctggttt acaccattca
    41701 tggttatctg ggctggcttt cagtcgccat tatgggcgcg ctgtggcctc tgctggtaat
    41761 gaccgggatg caccgcgtct ttacgccaac catcattcag accattgccg aaaccggcaa
    41821 agaagggatg gtcatgccgt cagagatcgg cgctaacctg tcgctgggcg gttcatcact
    41881 ggcggtggcg tggaaaacga aaaacccgga actgcgccag acggcgctgg ctgcggcggc
    41941 atcagccatt atggcgggga tttccgaacc ggcgttatac ggcgtggcga tccgcctgaa
    42001 acgtccgctt atcgccagtc ttatcagcgg ttttatttgc ggcgcggttg ccggtatggc
    42061 ggggcttgcc agccactcaa tggcagcgcc ggggttattt accagcgtgc aattcttcga
    42121 tccggcgaat ccaatgagca tcgtctgggt gttcgcggtc atggcgctgg cggtggtgct
    42181 gtcgtttatc ctcacactat tgctcggctt tgaggatatt cctgttgagg aagcggctgc
    42241 cgaggcgcga aagcatcaga gcgcacaacc gaccgtcgcc aaagaagtaa gtcttaattg
    42301 aggatgaaaa tgtcagtatt tccagaaggt tttttatggg gcggcgcgct tgccgccaac
    42361 cagtctgaag gtgcgttccg tgaaggtggc aaaggactga ccacggtcga tatgatcccg
    42421 cacggcgagc atcgaatggc ggtgaaactg gggctggaaa aacgttttca gttgcgcgat
    42481 gacgagtttt atcccagcca tgaggcgacg gatttttatc atcgttataa agaagatatc
    42541 gccctgatgg cagagatggg attcaaggtt ttccgtacct caattgcctg gagccgcctc
    42601 tttccgcagg gcgatgaaat cacgcccaat cagcagggca ttgcttttta ccgttctgtc
    42661 tttgaagagt gtaaaaagta cggtatcgaa ccgctggtca cgttgtgcca cttcgatgtg
    42721 ccgatgcatc tggtcaccga atatggctcc tggcgtaacc gcaagctggt ggagtttttc
    42781 agccgctacg ccagaacctg ctttgaagca tttgatggtc tggtgaaata ctggctaacc
    42841 ttcaatgaaa tcaacattat gttgcatagc ccgttctccg gcgcgggtct ggtgtttgaa
    42901 gaaggtgaaa atcaggatca ggtgaaatat caggccgcgc atcaccagct ggttgccagt
    42961 gcgctagcca ccaaaatcgc ccatgaggtt aacccgcaaa atcaggtggg ctgtatgctg
    43021 gcgggcggta acttctaccc ttacagctgc aaaccggaag atgtctgggc ggcgctggag
    43081 aaagatcggg aaaacctgtt ttttatcgat gtgcaggcgc gcggcgcgta tccggcttac
    43141 tctgcccgcg tatttcgcga aaaaggggta accatcaaca aagcaccggg cgatgatgaa
    43201 atcctgaaaa acaccgtcga ttttgtctct ttcagctatt acgcctcgcg ctgcgcctcg
    43261 gcggagatga acgccaacaa cagcagtgcg gcgaacgtgg tgaaatcgct gcgtaatccg
    43321 tatctacagg tgagcgactg gggctgggga attgatccac tcggtctgcg tatcaccatg
    43381 aatatgatgt acgatcgtta tcagaagccg ctgtttctgg tggaaaacgg cctcggcgca
    43441 aaagatgaac ttgctgccaa tggcgagatt aacgatgact atcgcatcag ctatttacgc
    43501 gaacatatcc gcgcaatggg cgaagcgatt gcagatggca ttccgctgat gggctacacc
    43561 acctggggct gtattgattt agtttccgcc tctacgggtg aaatgagcaa acgctacggt
    43621 tttgtctacg tagaccgtga cgacgcaggc aacggcacgc tgacgcgcac gcgtaagaaa
    43681 tcgttctggt ggtataaaaa agtaattgcc agtaatgggg aagatttaga gtaagtaacc
    43741 gtgccggatg cggcgtgaac gccttatccg gcctacatag ttcgtgcga
//
LOCUS       DAZGAN020000027        43120 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGAN020000027 DAZGAN020000000
VERSION     DAZGAN020000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 43120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.027"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            247..1344
                     /gene="mltA"
                     /locus_tag="ACHKYO_RS08665"
                     /old_locus_tag="pgaptmp_001733"
     CDS             247..1344
                     /gene="mltA"
                     /locus_tag="ACHKYO_RS08665"
                     /old_locus_tag="pgaptmp_001733"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HGU4428592.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DTWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1421..2227
                     /gene="tcdA"
                     /locus_tag="ACHKYO_RS08670"
                     /old_locus_tag="pgaptmp_001734"
     CDS             1421..2227
                     /gene="tcdA"
                     /locus_tag="ACHKYO_RS08670"
                     /old_locus_tag="pgaptmp_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HGU4428593.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2278..2721)
                     /gene="csdE"
                     /locus_tag="ACHKYO_RS08675"
                     /old_locus_tag="pgaptmp_001735"
     CDS             complement(2278..2721)
                     /gene="csdE"
                     /locus_tag="ACHKYO_RS08675"
                     /old_locus_tag="pgaptmp_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HGU4428594.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TVAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2721..3926)
                     /gene="csdA"
                     /locus_tag="ACHKYO_RS08680"
                     /old_locus_tag="pgaptmp_001736"
     CDS             complement(2721..3926)
                     /gene="csdA"
                     /locus_tag="ACHKYO_RS08680"
                     /old_locus_tag="pgaptmp_001736"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HGU4428595.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4118..4345
                     /gene="ygdI"
                     /locus_tag="ACHKYO_RS08685"
                     /old_locus_tag="pgaptmp_001737"
     CDS             4118..4345
                     /gene="ygdI"
                     /locus_tag="ACHKYO_RS08685"
                     /old_locus_tag="pgaptmp_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HGU4428596.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4696..5613
                     /gene="gcvA"
                     /locus_tag="ACHKYO_RS08690"
                     /old_locus_tag="pgaptmp_001738"
     CDS             4696..5613
                     /gene="gcvA"
                     /locus_tag="ACHKYO_RS08690"
                     /old_locus_tag="pgaptmp_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HGU4428597.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5632..6027
                     /gene="ygdD"
                     /locus_tag="ACHKYO_RS08695"
                     /old_locus_tag="pgaptmp_001739"
     CDS             5632..6027
                     /gene="ygdD"
                     /locus_tag="ACHKYO_RS08695"
                     /old_locus_tag="pgaptmp_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HGU4428598.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6020..7120
                     /gene="rlmM"
                     /locus_tag="ACHKYO_RS08700"
                     /old_locus_tag="pgaptmp_001740"
     CDS             6020..7120
                     /gene="rlmM"
                     /locus_tag="ACHKYO_RS08700"
                     /old_locus_tag="pgaptmp_001740"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HGU4428599.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7164..7895)
                     /gene="fucR"
                     /locus_tag="ACHKYO_RS08705"
                     /old_locus_tag="pgaptmp_001741"
     CDS             complement(7164..7895)
                     /gene="fucR"
                     /locus_tag="ACHKYO_RS08705"
                     /old_locus_tag="pgaptmp_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HGU4428600.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7953..8375)
                     /gene="fucU"
                     /locus_tag="ACHKYO_RS08710"
                     /old_locus_tag="pgaptmp_001742"
     CDS             complement(7953..8375)
                     /gene="fucU"
                     /locus_tag="ACHKYO_RS08710"
                     /old_locus_tag="pgaptmp_001742"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HGU4428601.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8377..9795)
                     /gene="fucK"
                     /locus_tag="ACHKYO_RS08715"
                     /old_locus_tag="pgaptmp_001743"
     CDS             complement(8377..9795)
                     /gene="fucK"
                     /locus_tag="ACHKYO_RS08715"
                     /old_locus_tag="pgaptmp_001743"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGU4428602.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9904..11679)
                     /gene="fucI"
                     /locus_tag="ACHKYO_RS08720"
                     /old_locus_tag="pgaptmp_001744"
     CDS             complement(9904..11679)
                     /gene="fucI"
                     /locus_tag="ACHKYO_RS08720"
                     /old_locus_tag="pgaptmp_001744"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGU4428603.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11712..13028)
                     /gene="fucP"
                     /locus_tag="ACHKYO_RS08725"
                     /old_locus_tag="pgaptmp_001745"
     CDS             complement(11712..13028)
                     /gene="fucP"
                     /locus_tag="ACHKYO_RS08725"
                     /old_locus_tag="pgaptmp_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HGU4428604.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13575..14222
                     /gene="fucA"
                     /locus_tag="ACHKYO_RS08730"
                     /old_locus_tag="pgaptmp_001746"
     CDS             13575..14222
                     /gene="fucA"
                     /locus_tag="ACHKYO_RS08730"
                     /old_locus_tag="pgaptmp_001746"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGU4428605.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14250..15398
                     /gene="fucO"
                     /locus_tag="ACHKYO_RS08735"
                     /old_locus_tag="pgaptmp_001747"
     CDS             14250..15398
                     /gene="fucO"
                     /locus_tag="ACHKYO_RS08735"
                     /old_locus_tag="pgaptmp_001747"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HGU4428606.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15453..16208)
                     /gene="xni"
                     /locus_tag="ACHKYO_RS08740"
                     /old_locus_tag="pgaptmp_001748"
     CDS             complement(15453..16208)
                     /gene="xni"
                     /locus_tag="ACHKYO_RS08740"
                     /old_locus_tag="pgaptmp_001748"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HGU4428607.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16320..17687)
                     /gene="sdaB"
                     /locus_tag="ACHKYO_RS08745"
                     /old_locus_tag="pgaptmp_001749"
     CDS             complement(16320..17687)
                     /gene="sdaB"
                     /locus_tag="ACHKYO_RS08745"
                     /old_locus_tag="pgaptmp_001749"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HGU4428608.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHSRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17745..19034)
                     /gene="sdaC"
                     /locus_tag="ACHKYO_RS08750"
                     /old_locus_tag="pgaptmp_001750"
     CDS             complement(17745..19034)
                     /gene="sdaC"
                     /locus_tag="ACHKYO_RS08750"
                     /old_locus_tag="pgaptmp_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HGU4428609.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19591..20955)
                     /gene="ppnN"
                     /locus_tag="ACHKYO_RS08755"
                     /old_locus_tag="pgaptmp_001751"
     CDS             complement(19591..20955)
                     /gene="ppnN"
                     /locus_tag="ACHKYO_RS08755"
                     /old_locus_tag="pgaptmp_001751"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HGU4428610.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21067..21915)
                     /gene="queF"
                     /locus_tag="ACHKYO_RS08760"
                     /old_locus_tag="pgaptmp_001752"
     CDS             complement(21067..21915)
                     /gene="queF"
                     /locus_tag="ACHKYO_RS08760"
                     /old_locus_tag="pgaptmp_001752"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HGU4428611.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            21983..22528
                     /gene="syd"
                     /locus_tag="ACHKYO_RS08765"
                     /old_locus_tag="pgaptmp_001753"
     CDS             21983..22528
                     /gene="syd"
                     /locus_tag="ACHKYO_RS08765"
                     /old_locus_tag="pgaptmp_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HGU4428612.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23150..23479
                     /gene="yqcC"
                     /locus_tag="ACHKYO_RS08770"
                     /old_locus_tag="pgaptmp_001754"
     CDS             23150..23479
                     /gene="yqcC"
                     /locus_tag="ACHKYO_RS08770"
                     /old_locus_tag="pgaptmp_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HGU4428613.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23479..24261
                     /gene="truC"
                     /locus_tag="ACHKYO_RS08775"
                     /old_locus_tag="pgaptmp_001755"
     CDS             23479..24261
                     /gene="truC"
                     /locus_tag="ACHKYO_RS08775"
                     /old_locus_tag="pgaptmp_001755"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HGU4428614.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24279..24728
                     /gene="yqcA"
                     /locus_tag="ACHKYO_RS08780"
                     /old_locus_tag="pgaptmp_001756"
     CDS             24279..24728
                     /gene="yqcA"
                     /locus_tag="ACHKYO_RS08780"
                     /old_locus_tag="pgaptmp_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGU4428615.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            25163..26515
                     /gene="gudP"
                     /locus_tag="ACHKYO_RS08785"
                     /old_locus_tag="pgaptmp_001757"
     CDS             25163..26515
                     /gene="gudP"
                     /locus_tag="ACHKYO_RS08785"
                     /old_locus_tag="pgaptmp_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HGU4428616.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26517..27857
                     /gene="gudX"
                     /locus_tag="ACHKYO_RS08790"
                     /old_locus_tag="pgaptmp_001758"
     CDS             26517..27857
                     /gene="gudX"
                     /locus_tag="ACHKYO_RS08790"
                     /old_locus_tag="pgaptmp_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HGU4428617.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHSAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHIGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            27878..29218
                     /gene="gudD"
                     /locus_tag="ACHKYO_RS08795"
                     /old_locus_tag="pgaptmp_001759"
     CDS             27878..29218
                     /gene="gudD"
                     /locus_tag="ACHKYO_RS08795"
                     /old_locus_tag="pgaptmp_001759"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HGU4428618.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGRTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQHRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29450..32206)
                     /gene="barA"
                     /locus_tag="ACHKYO_RS08800"
                     /old_locus_tag="pgaptmp_001760"
     CDS             complement(29450..32206)
                     /gene="barA"
                     /locus_tag="ACHKYO_RS08800"
                     /old_locus_tag="pgaptmp_001760"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HGU4428619.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32263..33564
                     /gene="rlmD"
                     /locus_tag="ACHKYO_RS08805"
                     /old_locus_tag="pgaptmp_001761"
     CDS             32263..33564
                     /gene="rlmD"
                     /locus_tag="ACHKYO_RS08805"
                     /old_locus_tag="pgaptmp_001761"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGU4428620.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33612..35846
                     /gene="relA"
                     /locus_tag="ACHKYO_RS08810"
                     /old_locus_tag="pgaptmp_001762"
     CDS             33612..35846
                     /gene="relA"
                     /locus_tag="ACHKYO_RS08810"
                     /old_locus_tag="pgaptmp_001762"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HGU4428621.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            35924..36172
                     /gene="mazE"
                     /locus_tag="ACHKYO_RS08815"
                     /old_locus_tag="pgaptmp_001763"
     CDS             35924..36172
                     /gene="mazE"
                     /locus_tag="ACHKYO_RS08815"
                     /old_locus_tag="pgaptmp_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HGU4428622.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            36172..36507
                     /gene="mazF"
                     /locus_tag="ACHKYO_RS08820"
                     /old_locus_tag="pgaptmp_001764"
     CDS             36172..36507
                     /gene="mazF"
                     /locus_tag="ACHKYO_RS08820"
                     /old_locus_tag="pgaptmp_001764"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HGU4428623.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            36578..37369
                     /gene="mazG"
                     /locus_tag="ACHKYO_RS08825"
                     /old_locus_tag="pgaptmp_001765"
     CDS             36578..37369
                     /gene="mazG"
                     /locus_tag="ACHKYO_RS08825"
                     /old_locus_tag="pgaptmp_001765"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HGU4428624.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37597..39234
                     /gene="pyrG"
                     /locus_tag="ACHKYO_RS08830"
                     /old_locus_tag="pgaptmp_001766"
     CDS             37597..39234
                     /gene="pyrG"
                     /locus_tag="ACHKYO_RS08830"
                     /old_locus_tag="pgaptmp_001766"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HGU4428625.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39322..40620
                     /gene="eno"
                     /locus_tag="ACHKYO_RS08835"
                     /old_locus_tag="pgaptmp_001767"
     CDS             39322..40620
                     /gene="eno"
                     /locus_tag="ACHKYO_RS08835"
                     /old_locus_tag="pgaptmp_001767"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HGU4428626.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(40680..41552)
                     /gene="ygcG"
                     /locus_tag="ACHKYO_RS08840"
                     /old_locus_tag="pgaptmp_001768"
     CDS             complement(40680..41552)
                     /gene="ygcG"
                     /locus_tag="ACHKYO_RS08840"
                     /old_locus_tag="pgaptmp_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU4428627.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLDSIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(41566..41706)
                     /gene="yqcG"
                     /locus_tag="ACHKYO_RS08845"
                     /old_locus_tag="pgaptmp_001769"
     CDS             complement(41566..41706)
                     /gene="yqcG"
                     /locus_tag="ACHKYO_RS08845"
                     /old_locus_tag="pgaptmp_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HGU4428628.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            41845..42516
                     /gene="queE"
                     /locus_tag="ACHKYO_RS08850"
                     /old_locus_tag="pgaptmp_001770"
     CDS             41845..42516
                     /gene="queE"
                     /locus_tag="ACHKYO_RS08850"
                     /old_locus_tag="pgaptmp_001770"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HGU4428629.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT        10776 a        10363 c        11350 g        10631 t
ORIGIN      
        1 tatgagcccg acgagctacc aggctgctcc accccgcgtc cgtggatgcg cactatactc
       61 cgctcgcgtt ttcatgcaac ctttttttta ccccgatcac ggatttagca tgattttgaa
      121 caaaaacaaa ccactaaagc ctattttttg tgcaaaattt gcgtcaggga gaggctgctc
      181 atttcattaa acgcggcggt ttgttatctt cgttgcgcct tattttttaa cctgaagaag
      241 agaacaatga aaggacgttg ggtaaagtac cttcttatgg gcacggttgt ggcaatgctt
      301 gccgcctgct cttccaaacc aaccgatcgc ggacagcaat ataaagacgg gaaatttacc
      361 cagcctttct ctctggtgaa ccagccagat gccgttggcg cgccgattaa cgccggtgat
      421 tttgccgagc aaattaacca tatccgtaat tcgtcaccgc gtctgtatgg caaccagagt
      481 aatgtttata acgcggtgca agagtggctg cgcgcaggcg gtgatacccg caatatgcgc
      541 cagttcggca ttgatacctg gcagatggaa ggtgccgaca actatggtaa cgtgcagttt
      601 acaggctatt acacgccggt aattcaggcg cgccataccc gccagggcga gttccagtat
      661 cctatttacc gtatgccgcc aaaacgtggt cgtctgccgt ctcgtgcgga gatctacgca
      721 ggggcgctga gtgataaata tattctcgct tacagcaact ccctgatgga taacttcatt
      781 atggatgtgc agggtagcgg gtatatcgac tttggtgatg gcagtccgct taactttttc
      841 agctatgcag ggaaaaacgg tcatgcctat cgcagcattg gtaaggtgct gatcgaccgt
      901 ggcgaagtga aaaaagaaga tatgtcgatg caggcgattc gtcattgggg cgaaacacac
      961 agtgaagccg aggttcgcga gctgctggaa cagaacccgt ctttcgtttt ctttaaaccg
     1021 caatcttttg caccggtgaa aggggcaagt gcggtgccgc tggttggccg tgcttctgtt
     1081 gcctccgatc gttcaattat tccgccaggt actaccttgc tggcagaagt gccgctgctg
     1141 gataataacg gcaaatttaa cggtcagtac gaattgcgtc tgatggtggc gctggatgtc
     1201 ggtggcgcaa tcaaaggcca acacttcgat atctatcaag ggatcgggcc ggaagccgga
     1261 catcgcgcag gttggtacaa ccactatgga cgtgtctggg tgctgaaaac cgccccgggc
     1321 gcaggtaacg tctttagcgg ctgatgtgtt attctgtata ccgtttttac ggatacgcca
     1381 gggtgaggga taacctcacc ctttttaatt ctgaggtgct atgtctgtgg taattagtga
     1441 tgcatggcgt cagcgttttg gtggcacagc gcgtctgtat ggtgaaaaag cgttgcaact
     1501 gtttgctgac gcgcatattt gtgtggttgg tatcggtggt gtcggttcct gggcggcgga
     1561 agcgctggcg cgcacgggga ttggcgcaat cacgcttatc gatatggatg atgtgtgcgt
     1621 caccaatacc aatcggcaaa ttcacgctct gcgcgataac gtcgggctgg caaaagcgga
     1681 agtcatggcg gagcgtattc gccagattaa cccggagtgc cgtgtaacgg tggtggatga
     1741 tttcgtgacg ccggataacg tagcgcagta tatgagcgtg ggttattcgt acgtgattga
     1801 tgccattgat agtgtacggc ccaaagcggc gctgattgct tattgtcggc gcaataaaat
     1861 cccgctggtc acgaccggtg gcgcgggtgg gcagattgac ccgacgcaga ttcaggttac
     1921 cgatctggcg aaaacgattc aggatccgct ggcggcgaag ttgcgcgagc gcctgaaaag
     1981 cgattttggc gtagtgaaaa acagtaaagg taagctcggc gtcgattgcg tgttttctac
     2041 tgaagcgctg gtgtacccgc agtcagacgg tactgtgtgt gcgatgaaag ccacggcaga
     2101 agggccgaag cggatggatt gtgcatctgg atttggcgcg gcaacgatgg tgaccgccac
     2161 ctttgggttt gttgcggttt ctcatgcgct gaagaagatg atggcgaaag cggcgcgtca
     2221 gggttaaaca atcaggtcgg atgcgacgcg aacgtcttat ccgacctggt tctgtgctta
     2281 aacctgcttc gccgcagcga taatcgcctc gcttaacgca ttcaacccct ggctgcgtga
     2341 ggcgctaagc tgtgcacgca atcccagctc atcaaacaat gccagcggcg actgtgcctg
     2401 caattcggcg acggttttcc cctcaacggc agtcaacaac accgccaaca ggccgcgcac
     2461 aatgcgccct tcgctgtcgc caaagaagtg cattttaccg ttttcagtca cggtatatcc
     2521 cagccagacg cggttttcgc atccggcaat ctctttagcc tgcgctttta actcgtctgg
     2581 caaagccgga agctgtttcc ccagcatgat caactggcga tatttatctt cccattgcga
     2641 caacggtgcg aaggtattgc gtaacgtttc tgcggttacg gttgtgccga acggatgtcc
     2701 ggcgaattgc gggtttgtca ttaatccacc aataattcca gcgcgcggtc aacggcattc
     2761 accagcgcat ccacatcact ctttgtatta tatggcgcaa aagaggcgcg cagtgtgccg
     2821 gttacgccta attctgccag tagcggctga gcgcaatgct gcccggcccg cagggcaata
     2881 ccgtactccg ccagcagcgt caccatatcg ctatgatgaa cgccagcaaa atcaaaggcc
     2941 agcaggctgg aatcctggca gcggaatgaa cgaaagccgg gacgtttcgc cagcgcatct
     3001 tccgccagcg ttgctaagct acggctccag ctttcggcct ggttgatatc gtaatctgcc
     3061 agccattcca gcgccgcgct taatcctatg acaccagcga catttggcgt tccagcttcc
     3121 agtttccacg gcgcagattg agtcgtgaag ccgtcaaaac tcacttcgtg aaccattttg
     3181 ccgccgccca gccagggcga catcgcctcc agcagttctg atttaccata cagcacgccg
     3241 atacctgtcg ggccatacag tttgtgacct gaaaaagcat agaaatcaat atccagttgc
     3301 tgaacatccg cggggaaatg cactgccccc tgagcaccat caaccatcac caccatcccg
     3361 gctgaatgag caaaggtaat cgctcgcgcc agatccgggc aaccgccagt aacgttcgac
     3421 atctgaccca acgccagaat ccgactacgg ggagtaatca gttctggcaa caaatcgaca
     3481 tccggcagtc gctgcgcatt aagcggcaat ttcaccactt tggctccagt ttgttgggcg
     3541 accatcagcc aggggacgag gttagcgtgg tgttctgcca cgctgacaat aatctcatcg
     3601 cccggttgca gacgcggacg cgcatagcat tgtgccacca tgttgatgga ttcagtggtg
     3661 ccgcgcgtcc agacgatagt tttatcatcc ggtgcattca gtaattgcgc cactttctct
     3721 cgtgcagctt cataacgcgc ggtcaggcgt tgggcttcgg caaactggct gcgatggacg
     3781 tttccggcgc tcaggctgta aaactgtcga gtggcttcaa ccacggcttc aggtttaagc
     3841 gcggtcgcgg cgctatcgag atagacgccc gcatcctgta gtgcgggaaa ctgggcgcga
     3901 aactgcgcgg gattaaaaac gttcatggta ctcctcggct tgatgcaccg atcgtgacgc
     3961 aaaattcact ttgatacaag gagagtgcta accatcagaa ttatctggat atcctggcga
     4021 agccgcgtaa gtaacatatg ctgaatagtg ttaggcgtta attttagcct gttaatcaat
     4081 actattaaga tttattaatc gctttaagga gaagatgatg aaaaagactg ccgcaattat
     4141 ttctgcctgt atgctgactt ttgccctgag cgcctgttcc ggttcgaact atgtgatgca
     4201 caccaatgac ggacgtacca tcgtctctga cggcaaacca cagactgata acgataccgg
     4261 tatgatttcg tataaagacg ctaatggcaa caaacagcag atcaaccgta ctgacgtgaa
     4321 agagatggtc gaactggatc agtaattcgc gatcgcaagg taaaaaaaag caccgcaatt
     4381 aggcggtgct acattaatca ctatggacag acagggtaaa tgtacaggaa gtgaaaaaag
     4441 gtagctttgc taccatggtc tgaatcgcag accaattgca aacacaacaa cacaacatca
     4501 caaccgtaag ccaaaagttc accagaacac gcattccgat aaaacttttc gttccggctc
     4561 aggaagtgcc gccactatag gtatttgctg gtagaagctc aacggacaat ttataatggc
     4621 tcagattaaa aaaactaata ggttacacag tgtgatctaa ttgttaaatt catttaacat
     4681 caaagtttaa aagccatgtc taaacgatta ccaccgctaa atgccttacg agtttttgat
     4741 gccgcagcac gccatttaag tttcactcgc gcagcagaag agctttttgt gacccaggcc
     4801 gcagtaagtc atcaaatcaa gtctcttgag gattttttgg ggctaaaact gttccgccgc
     4861 cgtaatcgtt cactcctgct gaccgaggaa gggcaaagct atttcctcga tatcaaagag
     4921 atattttcgc aattaaccga agcgacgcgt aaactccagg cccgtagcgc caagggggcg
     4981 ttgacggtca gtttactccc cagtttcgcc attcattggt tggttccgcg actttccagc
     5041 tttaattcag cttatccggg aattgacgtt cgaatccagg cggttgatcg tcaggaagat
     5101 aagctggcgg atgatgttga tgtggcgata ttttatggtc ggggcaactg gccggggcta
     5161 cgggtggaaa aactgtacgc cgaatattta ttgccggtgt gttcgccgct actgctgaca
     5221 ggcgaaaaac ccttgaagac cccggaagat ctggctaaac atacgttatt acatgatgcg
     5281 tcacgccgtg actggcagac atatacccga cagttggggt taaatcatat caacgttcag
     5341 caagggccaa tttttagtca tagcgccatg gtgctgcaag cggctattca cgggcaggga
     5401 gtggcgctgg caaataacgt gatggcgcaa tctgaaatcg aggccggacg tcttgtttgc
     5461 ccgtttaatg atgttctggt cagtaaaaac gctttttatc tggtttgtca tgacagccag
     5521 gcagaactgg gtaaaatagc cgcctttcgc caatggatcc tggcgaaagc cgctgctgaa
     5581 caagaaaaat tccgctttcg ttatgaacaa taatttacgt agggtacgac catgaccagc
     5641 cgttttatgc tgattttcgc cgccattagc ggcttcattt ttgtggctct gggcgctttt
     5701 ggcgcgcatg tgttaagtaa aaccatgggg gccgttgaga tgggctggat ccagaccggc
     5761 ctcgaatacc aggcgtttca tacgctggcg atcttaggtc tggcggtggc aatgcagcgt
     5821 cgcatcagta tctggtttta ctggagtagc gttttcctcg cgttaggcac ggtgttgttc
     5881 agcggcagcc tttattgcct ggcgctgtcc catctgcgtt tgtgggcgtt tgtcactccg
     5941 gttggcggcg tgagcttcct cgcgggctgg gcgttaatgt tagttggtgc tatccgttta
     6001 aagcgcaagg gcgtaagtca tgaataaggt tgtattgctg tgccgtccgg gctttgaaaa
     6061 agagtgcgcc gcagaaatta ccgataaagc cggccagcgg gaaattttcg gttttgcccg
     6121 cgtgaaagag aatgcgggtt atgtcattta tgaatgttat caacctgatg atggcgataa
     6181 gttaatccgt gagctgccgt tcagttcatt aatttttgcc cgccagtggt ttgtggtggg
     6241 ggaactcctg cagcatttgc cgccagaaga tcgtattacc cccattgtcg gcatgttaca
     6301 gggcgtagta gagaagggcg gtgaactgcg tgttgaagtt gccgatacca acgaaagcaa
     6361 agagttactg aaattctgcc gtaaatttac tgttccgcta cgcgctgcct tgcgcgatgc
     6421 cggggtgctg gcgaactatg aaacgccgaa gcgtccggtt gtgcatgtat tctttattgc
     6481 accaggctgc tgctataccg gttactcata cagcaacaat aattcgccgt tctatatggg
     6541 cattccgcgc ctgaaatttc cggcagatgc gccgagtcgt tccacgctca aactggaaga
     6601 ggcatttcat gtgtttattc ctgcggatga gtgggatgaa cgcctggcga acgggatgtg
     6661 ggcggtggat ttaggcgctt gccctggcgg ctggacctac caactggtga agcgcaacat
     6721 gtgggtttat tccgtcgaca acggcccgat ggcgcaaagt ctgatggata ccggacaggt
     6781 gacgtggctg cgggaagacg gtttcaaatt ccgtccgacg cgcagcaata tctcctggat
     6841 ggtatgcgat atggttgaaa aaccggcgaa agttgcggca ttgatggcgc agtggctggt
     6901 taatggctgg tgccgtgaaa ccattttcaa cctcaaactg ccgatgaaaa aacgctacga
     6961 agaagtgtca cacaatctgg cgtatattca ggcacagctt gatgaacatg gcataaatgc
     7021 tcagattcag gcgcggcagt tgtatcacga tcgcgaagaa gtgacggtgc acgtccgccg
     7081 catctgggct gcggtgggtg gtcgtcgcga cgagcgataa caggcaaggc cggggcgtcg
     7141 tctccggcct gctacccttt ccgtcaggct gttaccaaag aagttgcaac ctggcgctca
     7201 tcagaaataa tgtgcgttac ctcatccaga tgcccgatgc gggcttcccc tgaacgatta
     7261 aatttacttt tatcaatcaa taacaacgat tgcgcggcac gttttaatag catcgatttg
     7321 taatcagcgt tgatcgcatt ggagtcccac agtgcgccgc tgctatcgat cccttcacaa
     7381 gaaaaaataa acagatcgat ttccagcgat ttaagttggg aaatcagcga gggattgacg
     7441 taacagccat atttgcgctc aagtgtgccg ccggaactga tcagttgaat gcgttcgcgt
     7501 ttaccgagtt catggcaaat cggatggcta ttggtgaaga cctgaatgtt gatgtcaggc
     7561 aactggcgtg ccagatacca gcaagttgaa ctggcgtcta aggctatcac catcccttct
     7621 tcaatccacg cgagcgcctc gcgcgcgata tctgctttat gcgcataatg gcttttcagc
     7681 ctgatgtgaa agggatcgcc actgtcttga ttttgacggt ggatatattt agcgcgtcca
     7741 tgattgcgca gaattttacc ctgcgtctgt aattcattga gatcgcgacg aatggtttct
     7801 ttactgacct ttagctgttc agagagagct tccgtggtca ggctggtatg gttcagcagc
     7861 aggtcgacta tcgcttgctg gcgtgccgct ttcatatttt tcaccccatc attcgataac
     7921 cgcccgcgtc atgcgggcgt accggtatga gattacggtg ttaccccttt ttttaaaaga
     7981 atattcccgt acttcgctcg ttcgcctgtg ataacgatcg caaaggcttt ttgcgcccgt
     8041 tcataaaacg caaaacgatt gatgcggatg atgtcaggac acggggcttg tagtgaaagc
     8101 gcattacggt aacgtcgttc tacttcagga tcgagagtgt caccttctac cgccgccatc
     8161 atcaccagcg gcggtgcata actgtccagt tcaaataacg ggataatcgc ctggagcaag
     8221 tcgctcacca acaggccatc agcgcggatc acctgcggtc ccatcgaatg ggcgggaaag
     8281 tgagcatcgg aaaaaataat ttcatctcca tgtcccatct ctgccagcac tttcaatagt
     8341 tcgggagaaa ttaacggcga aattgttttc agcatttcac acttcctcta taaattcagg
     8401 ttcagtttgc gggtagaaat aacggtactg ataatgaatc tgtgcgcggg cttcttccgg
     8461 gctgttaaat tcccctacgc cataccaacc gaacagcgca gctcctgcga cggtcgtttc
     8521 ggcgtcgtcg agaactttta ccggaatatc aagcatattg gctttaatct gattccacaa
     8581 tgtgttgcga cttcctccac cgactaacaa taattcagag gccttaaagt gcccgatttt
     8641 ttccagcatc tgtagattgc gctgtaattg cgcagttaac ccttccagcg ccgcgcgata
     8701 gaaatgcccc cgcgtggtat taagcgtcac tccttgccag ccagcgttct gacacgacaa
     8761 taaatcacac tgcattttta cgccatccgc gccaggcgcg atcagacgag cttcttcaat
     8821 caacatttgc cagggtgttt cagccgtcca gaacagtttt ctcacccatt ccagcacgcc
     8881 ggatgccagc cattgcatac ctgggttata caaccctgcc tggctatcca gttcgcaggt
     8941 ggaaccggcg tactgactta acagcgaagt atcaacctgg gcgctacgga ccattaaaat
     9001 ttcccatgta ccggaagaga gcacgggttc attttggtcg gcaccagcgc caaaaagggc
     9061 gaactgggta tcgtgacctg cggaaatcac cggtatgcca acgggtaagc cgagcattgc
     9121 tgcggcgctg ttctgtagcg taccaatctg ttcacccgct tccaccagac gagggaagag
     9181 tcggcgtgga ataccggtgg cttgtaaaat ttgcggactg aaatcgcgtt gctggatatc
     9241 cagcatctgg ctggttccgg ccatcgtgat atcagtagtg aattcgccgg ttaaacggtg
     9301 gttaatcagc gacgaaataa agagccaggc gtgcgcgcgt tccagcagtt gtggatgatt
     9361 ttctttcaac cacaccaact tatataacgt attgaaacta aaggctccga cgccagaaat
     9421 agcctgcaac cgctgtgcgg agattaaccg ttcaatattg tccataaccg ctgctgttcg
     9481 cggacatttc cagctaatga tcggatagag cagattgcct tgcttatcta ccagagcgcc
     9541 atccacacca aaggtggtga cggcgatacc gcggatgtgg cattcagtca gttcactatt
     9601 gatttgccga cagcaatcag caaagcgttg caaaatggcg tctaaagacc actggtgcca
     9661 ggtgttgttt tccatcgcga tatcgctggc attaggcgtt gaggcgcggg caacaatttt
     9721 tccctgccga ttaaccgcga tggccctgac attggtcgcg ccacagtcga gtaccaggat
     9781 aacttcttgt ttcataatcg ctcctgcaat atagccggat aacattgttt atccggctaa
     9841 ccactcttgc ttgttcaggc ccggtagccg gagcgaccgg gcatcacatc agggagtaat
     9901 gtattaacgc ttgtacaacg gaccgtagtt ctggcaagcg cggtaatcct ggccttcaat
     9961 atccatgccg tgcgcagccc aggcagaagg acgatacact ttggtctctt caacgttgtg
    10021 catacatacc gggatacgca gcatggaggc aagagtgata aagtctgcgc caacgtggcc
    10081 gatggtcaga actccgtggt tggcacccca gttcgccatt accgaataca catccgtaaa
    10141 cggcccttta ccagtgaggc gcggtgcaaa ccaggtggtt ggccaggttg agttggtgcg
    10201 tttgttgagg atgtcatgca catccttcgg caattccacg ctccagcctt ccgcgatttg
    10261 cagtaccggt cccaggcctt tgatgatgtt gacacgagtc atggtgaacg ggacgccgcc
    10321 ttcggtaagg aagcgggaag agtaaccgcc gccacggaag tattcgtgga tcgccgggca
    10381 ccattcggta gcggcgaggc aagcgtcagc ctcttgctga gagatttccc agtgcggctt
    10441 catcgtcggg ttaccttcgc tgtcgcgttg tttacaggaa ccgtccagcg cagcagaacc
    10501 tgagttgatc aaatggatga tgccgtgttc tgccagtcca tcgagtttat gccccgttac
    10561 acgctcaatt gcttctggtg accagtaggt acgcacatcg gcaaatacct gagcggtgcc
    10621 ggtgagctgg tgacccatta gcattgccac gccgttaaga ctgtcgtttt cggtcgccac
    10681 gacaaagggt tcgcgcacgc cattccagtc aaatgaactg ttgaggatcg cttcggcggt
    10741 gtcaccattg ggatattgat cggtccagtg acgttgcccc tggaagcccg cagcgatggc
    10801 gttgtagcca agtgattctt ccacgcgacc aatatcggcc agtttgctgt tgccttgcat
    10861 catgtcgcgg atacacatcg ccatcagtaa actttcgcgc agaactgcgc ggctttgctc
    10921 ggcattgcgt tgatactgtt tgttattttc atcttcgcca tagcggaagt ttttatcagc
    10981 ccaggccagc gccatttcca gttcggcttc gtcgtaaatc ttctgatcga tacggcgacg
    11041 cagttcggtc atatccaccg cctgaacttt cattcccagc caggattcaa agaagttgtg
    11101 atcaacaatg gaaccggcga tacccatcga aacgccgccc agcgacagat agcttttacc
    11161 tttcatgctg gcgacggcca aaccggcgcg ggcaaagcgc agcagttttt cttcaacatc
    11221 ggcaggaatc gatgtgtcat cggcatcctg aacgtcatga ccgtaaatgg agaatgccgg
    11281 gatgcctttc tggctgtgag ctgccagagc cgctgccagg taaacagcgc cgggacgttc
    11341 agtgccgtta aagccccaaa tggctttcgg gcgggttgga tccatgtcga tggtttcact
    11401 gccatagcac cagcaaggcg ttaccgtaat ggtgaggcct acattctgac tgctgaattt
    11461 ttcttcgcaa gcagcggctt cagccatacc cgcgatacag gtatcggaaa tgacacactc
    11521 gacggcagct ccgcaggcat ggcgcagttt ctcggtcagc agtgcggccg tagctttcgc
    11581 catattcatt gtttgttctt caagcgactc acgaacaccc atgcgacgac cgtcaataac
    11641 cgggcggata ccaattttcg gtaagctgat ttttttcatt acagattcct cactttattc
    11701 ggaaaatatg ttcagttagt tgccgtttga gagcggaaac gggcaaagat aaagatgacc
    11761 gcgaagcaga gtgcggggat cagttcagca gtggggatgt tgcccgccgc gtcactgaca
    11821 aaacccatga ccggagtgac aataccgccg ccaatgatgg tcataacgat gaaggacgaa
    11881 ccgtatttag tgtcctggcc gagattctta atgcccagcg agaagattgt tgggtactga
    11941 atcgacataa aggcgctgca taaagtcagg gctattaagc ccacatgacc gccagcgaag
    12001 gctgagatca ggcacagtgc catagcgatt aatgcgtagg cggccaggac tttgtgtggt
    12061 gcgaagcgac tgatgagcca ggtaccggtg aaacgaccaa taaagaagca caccatggtt
    12121 ccggttaaat agttagcggc aaagcctgca gtcatacctg gaatttcttc tacagcgtag
    12181 cgaatcaaat agctccagca ggccgtttgt gcgccaacgt agcagaattg cgctaatact
    12241 gcccagcgcc agtggcgaat acgcgccagg cgagaaagcg atgcggagaa cgatccttgt
    12301 ttggcgtcac tgtgattatc actctgcaat gccgggaatt tcgtcagcat gatcagcagg
    12361 gcgaccagta acacgatagc cacgatgatc atataaggtg tctgtaccga taataccagg
    12421 ctgtgtttat acgcactcaa ttgctctgga gacattttat cgagaacgtc ttgcgattga
    12481 tgtggcacgt tagacaaaat aagactttgc ccaaagacaa ccgcgataat tgcgccaaac
    12541 gagttaaatg tttgcgcaag atttaagcgg aagtgaccac tgctttccgg ccctaatacc
    12601 gtaacaaaag ggtttgcggc agtttccaga caacctaatc cggctgcaat aataaatagg
    12661 ccaactaaaa ataaggtgta gttcattatt tctgcagcgg gccagaataa tgcagcgccc
    12721 aaggcatata aaaataatcc ggtaataatc cctgctttat aactgagttt tttcatcaat
    12781 atcccagcag ggattgggat aatgaaataa ccaaagtaaa aggccgattg gatcaggcca
    12841 gcctggaaat ttgtcagcgt aaaagcctgc tggaattgag gtaataaaat gtcgttaagg
    12901 ttattggcta ccgcccaaag aaaaaacagt gagcacagca gcgcgaatgg aataatgtaa
    12961 cttctgcttt gccctgcatc tttatctacc gcacggtaac tctgcgtttg tattgatgtg
    13021 tttcccatag catcctctta ggttcagaag cttaagcacg taatcggtta tgttttcaaa
    13081 agtgcaaaac cagcgagttc gggcttcata tcagtgaata tttgcctgtc tgaaatgaac
    13141 tcatggattc attttcaaat aaaaaacgct ctgtaatatg acggcggtca cacttattag
    13201 ctggaaggtg actttatgtg actaccatca ctttaataat cgaaatttca tgcccgtttc
    13261 agaaaataaa tcataaaaaa cacaaaccag atgaccgtat taattgttta tgccgaaaac
    13321 gggcattcct ggcttgagtg aatagaaaaa tgaccgtttt gataaatatt tttaagagcg
    13381 gtcattttta atggggttat aaaaagagaa cttgcagtta tttactgtga ttagttgaac
    13441 caggtcacaa aatataagct tgtgaatttc tttctggtta atcaggaaga agatggtggg
    13501 taattaaacg gctaattcaa tagtgtgaaa ggaacaacat tattgccctg ttttgaatca
    13561 gagagaggta gctaatggaa cgaaataaac ttgctcgtca gattattgac acttgcctgg
    13621 aaatgacccg cctgggactg aaccagggga cagcggggaa cgtcagtgta cgttatcagg
    13681 atgggatgct gattacgcct acaggcattc catatgaaaa actgacggag tcgcatattg
    13741 tctttattga tggcaacggt aaacatgagg aaggaaagct cccctcaagc gaatggcgtt
    13801 tccatatggc ggcctatcaa agcagaccgg atgccaacgc ggttgttcac aatcatgccg
    13861 ttcattgcac ggcagtttcc attcttaacc gatcgatccc cgctattcac tacatgattg
    13921 cggcggctgg cggtaattct attccttgcg cgccttatgc gacctttgga acacgtgaac
    13981 tttctgaaca tgttgcgcag gctctcaaaa atcgtaaggc aactttgtta caacatcatg
    14041 ggcttatcgc ttgtgaggtg aatctggaaa aagcgttatg gttggcgcat gaagttgaag
    14101 tgctggcgca actttacctg acgaccctgg cgattacgga cccggtgcca gtgctgagcg
    14161 atgaagagat tgccgtagtg ctggagaaat tcaaaaccta tgggttacgg attgaagagt
    14221 aatttcgtaa agcaacaagg agaaggatga tggctaacag aatgattctg aacgaaacgg
    14281 catggtttgg tcggggtgct gttggggctt taaccgatga ggtgaaacgc cgtggttatc
    14341 agaaggcgct gatcgtcacc gataaaacgc tggtgcaatg cggcgtggtg gcgaaagtga
    14401 ccgataagat ggatgctgca gggctggcat gggcgattta cgacggcgta gtgcccaacc
    14461 caacaattac tgtcgtcaaa gaagggctcg gtgtattcca gaatagcggc gcggattacc
    14521 tgatcgctat tggtggtggt tctccacagg atacttgtaa agcgattggc attatcagca
    14581 acaacccgga gtttgccgat gtgcgtagcc tggaagggct ttccccgacc aataaaccca
    14641 gtgtaccgat tctggcaatt cctaccacag caggtactgc ggcagaagtg accattaact
    14701 acgtgatcac tgacgaagag aaacggcgca agtttgtttg cgttgatccg catgatatcc
    14761 cgcaggtggc gtttattgac gctgacatga tggatggtat gcctccagcg ctgaaagctg
    14821 cgacgggtgt cgatgcgctc actcatgcta ttgaggggta tattacccgt ggcgcgtggg
    14881 cgctaaccga tgcactgcac attaaagcga ttgaaatcat tgctggggcg ctgcgaggat
    14941 cggttgctgg tgataaggat gccggagaag aaatggcgct cgggcagtat gttgcgggta
    15001 tgggcttctc gaatgttggg ttagggttgg tgcatggtat ggcgcatcca ctgggcgcgt
    15061 tttacaacac tccacacggt gttgcgaacg ccatcctgtt accgcatgtc atgcgctata
    15121 acgctgactt taccggtgag aagtaccgcg atatcgcgcg cgttatgggc gtgaaagtgg
    15181 aaggtatgag cctggaagag gcgcgtaatg ccgctgttga agcggtgttt gctctcaacc
    15241 gtgatgtcgg tattccgcca catttgcgtg atgttggtgt acgcaaggaa gacattccgg
    15301 cactggcgca ggcggcactg gatgatgttt gtaccggtgg caacccgcgt gaagcaacgc
    15361 ttgaggatat tgtagagctt taccataccg cctggtaaat gcgctgatgt gataatgccg
    15421 gatacgacgt ttgcggcgta tccggctcgc cgttaccgta ccaaccgcaa ttgctgtaaa
    15481 ttgccgtcga tatgcaaatc ggtttgtaag cgggcaatat cgcggcacag aaacgccatc
    15541 tctttatggg tttctaattt tttgcgccac ttttcggcaa ccgcatccag attctcatat
    15601 attccttcca gactctgaaa ctcgaccagc agctgcgtgg cgctttttgg tccgattccc
    15661 gcaacacccg gtacctttga actgctgatc cccgcaagtc cccagtaatc gggcaactgc
    15721 tgcggttgaa cgccaaattc tttatcgata aatggcgcat ccagccaacg tttctggaag
    15781 taatcacgaa tacgtaatgt cggtgaaagt aactgacagt agcctttatc tgtcgaaaca
    15841 atcgttgcct gatgcccggc ctgtgtcact ttgaccgcca gcgtggcggc taagtcatcg
    15901 gcttcgttgc cgctggttga ccagcacggg acgccgcgtt gctcaaaggc ggcgcgtaat
    15961 gcaggcatct cgtcgtgcaa ctcttccggc attggcggtc gacccgcttt gtaatctggt
    16021 aaacgctgat gacgccagcc gctactgcgg ttttcatcat caaaaacggc gaccgcgtgg
    16081 gttggctggc tgtgcataat gagctgatcg agcgcatgct ggcaggtttc gacacagggc
    16141 gatccctgaa cggcatgaat gcgacgaata agattcagtg catcgacaat aagcaaatga
    16201 acagccacga taaccccctt aaagatcagg cgataagggt aacattcgat gccggaacag
    16261 gctacggctg gatgagaaat cgggaagagg cctcgcaaaa cgaggcctct ggagagcgat
    16321 taatcgcagg caacgatctt cattgccagg ccgccgcgag aggtttcgcg gtacttggcg
    16381 ttcatatctt tacctgtttc gtacatggtt tcgataactt tatcgaggca gacgcgcggc
    16441 tcgctggtac ggcgtagcgc catacgtgcg gcgttcaccg cttttactgc cgcaatggcg
    16501 ttacgctcga tgcatggcac ctggacctgt ccggcgaccg ggtcacacgt cagaccgagg
    16561 ttgtgctcca tggcgatttc cgccgcgatg cacacctgcg ccgggcttgc acctaacagt
    16621 tctgccagac cagccgccgc cattgagcac gccacaccaa cttcaccctg gcagccgact
    16681 tcagcaccag aaattgacgc gttcatctta taaagagaac caatggcact ggctaccagc
    16741 aggtaacgag ccagtgagtt agcgttcact tcgcggataa acttgtcgta gtacgccagc
    16801 actgccggga taatcccgca cgcaccgtta gtcggcgcag tcaccacgcg tccgccagca
    16861 gcgttctctt cgttcactgc caatgcaaac atgttgatcc agtcaacaac cgccatcggg
    16921 tcagtggtgg ttttatcctg gctgaccagc atccggcgta gtgccgcagc acggcgtgga
    16981 acgcgcagtt tgccaggcaa cacgccttcg gtggaaatac cgcgctcaat accgccacgc
    17041 atgacttccc atacgttcgc caggtgctgt tccagctctt ctttgctgtg cagcgccagc
    17101 tcatttttca tcatcaggcc agagagtgac agcccggttt cctgacaatg tttttgcaga
    17161 tcggctgctg aactgtacgg ataaggaact tcaaccggtg cgctattctg ctggccaaaa
    17221 tgctcttcat caacgataaa gccaccgcca atagagtagt aagtctggct gtaaacgact
    17281 ttatcgcccg ccagcgcggt aatgcgcata ccgttttcat gcagagaaag gttgtctgca
    17341 tggaagttca tgcactgatc aaccgggaac tccacttcat gctgaccgtt tgccagcatc
    17401 aggcgactat gagtattcac atcctgaata aaaccaggga tggaatcgat atccacggta
    17461 tccggcaggt tacccgccag gcccataata atggcgatat cagtgtggtg gcctttaccg
    17521 gtcagagaga gcgagccgta cacgtcaacc accacgcggg tcacgtcttt aagcagatta
    17581 cgggcaatca gatcgtcggt aaattgttta cccgctttca ttggtccaac ggtatgagaa
    17641 ctggaagggc caatgccgat tttgaaaata tcgaatacgc taatcatagg aaatacatcg
    17701 cgttaaaacg gaggaagcgc cgcccgaaag cggcgcgaaa ggacttagct gaacagagag
    17761 tagaagattg cggagattgc aatcagaccc atcacgacaa cgaatacgtt gctgatgtga
    17821 ccgctgtact tacgcattgc cggtactttc tgaattgcgt acatcggcat caggaacagg
    17881 atcatcgcga tgattgggcc gcccagggtt tcaatcatac ccaggatgct cgggttcagg
    17941 gtggcaacaa tccaggtcgt taccagcatg aacagcgcag tgatacggtt cagcttgttg
    18001 atttcgatag acttaccttt accacgcaga gatttaatca ccataccgtt gaagccttca
    18061 cgtgcgccca ggtagtggcc gaggaaggat ttggtgatag cgataatcgc gataatcgga
    18121 gccatccacg cgataaccgg tgcgttaaag tggttagcca ggtaagacag aatcgagatg
    18181 ttctgctctt tagccgcagc caggtctgcc ggagtcaggc tcagtacgca gctgaagacg
    18241 aagaacatta cggtcagcac catcatgatg tgtgcgaatg caaggatctt cgagcatttc
    18301 tgttctgcca tatcgccgta ctcttcacgc ttcgcaacgg cgaaagaaga gatgatcgga
    18361 gagtggttga acgagaacac cattaccgga attgccagcc acagggtcat ccacagaccg
    18421 tttccggttg cagatgcagt gtccagagac agcgtttcca gtgctgcgcc gttccactgc
    18481 gggatgaggt acagagccag cagcatcagt acgccaacaa acgggaatac cagaatactc
    18541 atcgctttaa cgatcatctg ctcaccgaag cgaacgatgg tcatcatacc cacgatcagg
    18601 atcagcgaca gaatcgcacg cggcggtggc gtcataccca gctggtgaga catgaagctt
    18661 tcaacggtat tggtgattgc cacgctataa accagcagga tcgggtagat agcgaagaag
    18721 tagagcaggg taatcagttt acctgcgcca ataccaaagt gttcttctac aacctcggtg
    18781 atgtcttcgc ccgggttttt accagacagt acgaagcgag tcaggccgcg gtgagcaaaa
    18841 aacgtcatcg ggaacgcaag gatagccatg atgatcagcg ggatcatacc gccaacaccg
    18901 gcgttgattg gcaggaacag cacgcccgcg ccgattgccg tgccgtaaag gcccagcatc
    18961 cacatggtgt ctgtcttgcg ccaggcacta cgagagtctt tcgacgcaat cgtgctggtt
    19021 tgagtcgttt ccatctattt ctcctggagg aatgcaaata attcagcttt tagccagttc
    19081 agcccgggaa ggtgcctgac cgtaaaaatg aaatctgtct atcggcgatg gcttaatcat
    19141 ttaccgccgt aataatttga gggcggaaag atacacacaa gttatgtatc tatcagtgat
    19201 cttgatctca aatacaataa tctacttgga agatagatta tcccaacgct ttactctgta
    19261 aatcaaaagt aaaagagaat ttaagggcaa tatttgccca ttaaacgaca tctgacttct
    19321 tccggggcgc gcaatatatg taggttgaac tgaaaaaatc aatactttta gttaatcatg
    19381 gcgataattt ctttttcata gcgataaaat acggtttatg aaaataggga aacgtttgcg
    19441 ttgcaatata aattgtatga catattaact gtgatgatat ttgtcgcgtg agggtggtaa
    19501 gaaacattca tataacaaat gaaatgagtt gatgagtaag tgaccaaaaa cgagaaaacc
    19561 cggcaacaaa cgtcaccggg ggaggggaga ttacgtgcag atttcgtagc aagggatgta
    19621 ggctgagcct ggcaacttca tacgatgctg ggcaacaaaa ccctgtagca ggtcgtccat
    19681 acgacgcata atctctttat cgccgttgat tttgtaagga ccaaactctt caatggcgcg
    19741 aataccgact tcttttacgt tacccgccac aataccggag aacgcacggc gcaggtcggc
    19801 agccagcact tcaacaggtt gatccgggta aagcttcaga ttagccatat tctcgtgaga
    19861 cggctcaaac ggcatttgca aatctggcgc aatgcgcatt gaccagttaa agctgtaggc
    19921 atcgcctgta tcacggcgat tttctttcac cagcggcatc gattttttca tctgacgagc
    19981 gacttcagcg gcgtcatcaa tgatgatgcg gtaatggcgg cgcgcgtttt cacccagcgt
    20041 atgtacgaca aactcgtcca gtacgcggaa gtagtcggcg ctctctttcg ggccggtgag
    20101 gatcaatggt aaaacctgat ctttgttggc cgggttcatt aaaattccca gcaaatacag
    20161 caactcttct gccgtaccca caccgccagg gaagataatg ataccgtgag cgatacggac
    20221 aaacgcttcc agacgttttt cgatatctgg catgatgatc aattcgttga ccagcgggtt
    20281 aggcggttca gcggcgataa tcgacggctc tgtcatacca ataaaacgac tgtctttgta
    20341 acgctgctgc gcgtgtccga ccgcagcacc tttcatcggc gcttccatcg ctcccggacc
    20401 acagccggtg cagatattca gctcacgcag gcccagctgg tttccgacgc gacgggcata
    20461 caaatactcg ttttcgttaa ttgagtgacc gccccagcag accaccatat ttggcgcttc
    20521 acccacatgc agcgcgcgag cgttacgcaa gatggaaaag accaggttag tgatgtggac
    20581 ggagttgtcg agattcaggt tgggaaaacg aacggtatta tggatttgcc cgtaaacgaa
    20641 gagaatgtct cgcagaaccg cgaacaagtt ggcctgcaaa gcgcgaataa ttcgcccatc
    20701 gacaaaagcc tcttccgggg gattaatcag ttccagcttt acgccgcgtt cacggcgcag
    20761 gacgttaata tcgaaatttt caaaacgaga cagcaattct ttgctgttat cggtcaaact
    20821 accggagttc agtacggcaa gtgaacagtt gcgaaacagt tgatagaggt cgctgctggc
    20881 ggtgcgttta agcatatcca cttccagctg cgacaacata tccatggagc caagcgggct
    20941 aatatgtgta atcaagaaaa actccttatg ggacgaatat cgtcattccc tttgattaca
    21001 atagccctgc ctgatgacct ttcacaacct tctgcgtgaa tccagcacgc aaaattgaga
    21061 aaaaatttat tgccgaacca gtcttgtggt cgatgggaca aaatcgctat tactgcgcca
    21121 cgggttaatg tccagaccgc cacgacgggt ataacgtgcg taaacgctta atttctctgg
    21181 ctggcagaag cgtaacaggt cattaaagat gcgttccacg cactgttcgt ggaactcgtt
    21241 gtgatgacgg aatgagacca ggtaacgcag cagtttttct ctgtcaattt ggcgtccacg
    21301 atactgaatt tggatcgaac cccaatctgg ttgatgggtg atcaggcagt ttgatttcag
    21361 caggtggctg acaagcgtct cttccactac tttttcaccg ctggtggcat tctccagata
    21421 gtcagtagtg aattcatagt tatcgatagt gatatcctgg tcatcaatgc aggtgccatt
    21481 gaaatgacct atcggctggc cttccagttc atcaagacga tataacgcca cgctaacctt
    21541 accctgggcg caagtgctta agtcgcgctc cagcgtctgg cgcacctcat cccagttatt
    21601 aaaacgcgtc tggttaaaac tgttgagata gagcttaaaa ctcttcgact caatcagatt
    21661 tgcgctggtg taatcaagtt caacgtgacc gacggcaacc tgcggcaaac ctttcgcatt
    21721 cagccaggaa agttcataca gcgtccagat atccgtaccg tgaaaaggca ggttatccgc
    21781 tttcagaccc agcgggtcgc gattcaggct gcgtggaacg ccttgcagta ggttggcgtc
    21841 ataggtatcc cggtaatcgg ttgattttcc aagagtcagg cccgcaagtg cctgatggtt
    21901 tgcataagaa gacatgtttc accgtcataa accagataaa ctaaacgaca agtgtatctg
    21961 gtttaagtga agagagaaat tagtggacga tttgaccgca caagccctga aagattttac
    22021 tgcacgctac tgcgatgcgt ggcatgaaga gcataaaagc tggccgttaa gtgaggaact
    22081 gtacggtgtt ccttcaccgt gcattatttc aactaccgaa gatgccgtat actggcaacc
    22141 ccagccgttt accggggaac aaaatgtaaa cgcggttgaa cgcgcttttg atattgtgat
    22201 acaacccaca attcatacct tttatacgac tcagtttgcc ggggatatgc acgcgcagtt
    22261 tggcgatatc aaactgacat tgctgcaaac ctggagtgaa gacgacttcc gtcgggtgca
    22321 ggaaaatctg attggtcatc tggtgactca gaaaaggcta aaactgccgc ccacgctatt
    22381 tatcgcgacg ctggaagaag agttagaggt catctcggtg tgtaatctct ccggtgaagt
    22441 gtgcaaagag acgctgggaa cacgtaaacg gacgcattta gcctcaaatc ttgcggaatt
    22501 ccttaatcaa cttaagcctc ttctgtaatc caaatacccc atctggttgt gagagatctc
    22561 ttacagactc tgtaggagat cgccaggaaa taagcgaata cttaaaaaga taagaatcgt
    22621 tattttcatt taaaatcaaa atgttgattg ttaattctta actttcatat gaaattttcc
    22681 ttaaggcatt gtctgtaagc gccttgtaag acttcgcgaa aaagacgatt ctatcttcgt
    22741 cgacagggag tcagacaacg aagtgaacat caggatgatg acacttctgc aggacacacc
    22801 aggatggtgt ttcagggaaa ggcttctgga tgaagcgaag aggatgacgc aggacgcgtt
    22861 aaaggacacc tccaggatgg agaatgagaa ccggtcagga tgattcggtg ggtcaggaag
    22921 gccagggaca cttcaggatg aagtatcaca tcggggtggt gtgagcagga agcaatagtt
    22981 caggatgaac gattggccgc aaggccagag gaaaagttgt caaggatgag cagggagcaa
    23041 caaaagtagc tggaatgctg cgaaacgaac cgggagcgct gtgaatacag tgctcccttt
    23101 ttttattcct gctatccttc gcggcagttt ttcttattga ggttgcttta tgaccactca
    23161 tgaccgcgtt cgtctccagt tgcaggcgct tgaagcgtta ctgcgtgaac atcagcactg
    23221 gcgaaacgat gaaccccagc cgcatcaatt taatagtacc caaccgttct ttatggacac
    23281 catggaacca ctggagtggt tgcagtgggt gctgatcccg cgaatgcacg atttgttaga
    23341 taacaaacag ccgttaccgg gcgcttttgc cgttgcaccc tattatgaaa tggcgctggc
    23401 gacggaccat ccgcaacgcg cgcttattct ggctgagctg gaaaaactgg atgctctttt
    23461 tgcggatgat gcgagctaat gctggaaata ctctatcagg atgaatggct ggttgcggta
    23521 aataaaccct ccggctggct ggttcaccgc agctggctgg atcgcgacga gaaagtagtg
    23581 gtcatgcaaa ccgtgcgtga tcagattggc cagcacgttt ttaccgctca tcgtctggac
    23641 cgacccactt ctggtgtgtt gttgatggga ttatccagcg aggccggacg gctgctggca
    23701 caacagtttg aacagcacca aatccagaaa cgttaccatg cgattgtgcg cggctggttg
    23761 atggaagaag cagttctgga ttatccactg gtggaagaac tggacaaaat cgctgataaa
    23821 tttgcccgcg aagataaagg ctcgcagcca gcagtgacgc attatcgcgg tctggcgacc
    23881 gtagaaatgc cggtagcgac cggacgttac ccgaccacgc gctacggcct ggtggaactg
    23941 gagccgaaaa ccggacgcaa acatcagctt cgccgacatc ttgcccattt gcgtcatccg
    24001 attattggcg atagcaaaca tggcgattta cgccagaatc gcagcggtgc tgagcatttt
    24061 ggcctccagc ggttaatgct acatgccagt cagttgtcat tgacgcatcc ttttactggc
    24121 gagccgctga ctattcacgc gggcctggac gacacctgga tgcaggcgtt atcacaattt
    24181 ggctggcgcg gtctgcttcc tgaaaatgaa agggttgagt tcagtgcgcc atcgggtcag
    24241 gatggagaga taagttccta atccagggag aaaacggtat ggcggaaatt ggtatttttg
    24301 tcggcaccat gtacggaaat tcgctgttag tggcggaaga agcagaagca attctgaccg
    24361 cacagggcca caaagcaacg gtatttgaag atcctgaatt aagcgactgg ctgccctatc
    24421 aggataagta tgttctggtg gttacgtcca cgaccgggca gggcgacctt cctgatagca
    24481 ttgtgccgct ctttcaggga atcaaagata gtctgggctt ccagccgaat ctgcgttatg
    24541 gcgtgattgc gctcggcgac agcagttatg taaatttctg caatggcggc aaacagttcg
    24601 atgccttgtt gcaggaacag agcgctcagc gggttggcga aatgctgttg attgacgcca
    24661 gtgagaaccc ggaaccggaa acggaatcaa atccgtgggt cgaacagtgg ggcacgctgt
    24721 tgtcctgata tgttcagcga gcggtaaatg tcgttttagc ggtgctgaat cgaatatttt
    24781 caggcaaatg ccagtaaaaa ctgcttcata gcgcggattt ttactggcgt ttgcctggag
    24841 tcaagcgatc catttcatac tcttctttat ttcttcgttt taacccttcc tttcttgttc
    24901 ttgttttcat ttccgtgaag tggattccac cgtccagggc taatgccaaa atcgggcctc
    24961 attgaacgca ttaatgttgt gttgttgcac ggtgagccgc tatggcgcgc tttttatact
    25021 gctattgcca gatataaaca cgcgccgtat tcggcgaacg acctataaaa acggcaaaaa
    25081 acaccctacg tcacctctga tttcctggct atgtcgcagt ccagagtgag cgtggctaac
    25141 gcgaattttc aggagtgcaa caatgagttc tttaagtcag gctgcgagca gtgtggaaaa
    25201 acgcacgaat gctcgttact ggatagtggt gatgttgttt atcgtcacat ccttcaacta
    25261 cggcgaccgc gctacgcttt ctattgccgg ttcggaaatg gccaaagata tcggccttga
    25321 tcccgtggga atgggctatg tgttctctgc cttctcatgg gcttatgtta tcgggcagat
    25381 ccctggtggt tggttgctgg atcgttttgg ttcaaaacgc gtctacttct ggtcgatctt
    25441 tatctggtcg atgtttacct tgctgcaagg cttcgtcgat atctttagtg gattcggcat
    25501 tatcgttgcc ctgtttactc tgcgcttcct ggtcgggctt gctgaagcgc catctttccc
    25561 cggcaacagt cgcattgttg cggcctggtt tccggcgcag gaaaggggaa cggcggtgtc
    25621 gatttttaac tccgctcaat acttcgcaac ggtgatcttc gcgccgatta tgggctggct
    25681 gacgcatgaa gtgggctggt cacacgtctt cttctttatg ggcggtctgg ggattgtcat
    25741 cagctttatc tggttgaaag tcatccacga gccaaatcaa catccggggg taaatcagaa
    25801 agagctggag tacatcgccg cgggtggcgc gctgatcaat atggatcagc aaaacaccaa
    25861 agttaaagtg ccgttcagcg tgaagtgggg gcagatcaaa cagctgctcg ggtcacggat
    25921 gatgatcggc gtttatatcg gtcagtactg tatcaacgcc ctgacttact tctttattac
    25981 ctggttcccg gtttatctgg tgcaggcacg cgggatgtcg attctgaaag cgggctttgt
    26041 ggcttccgtt ccggcggttt gcggttttat cggcggtgtg ctgggtggga ttatttccga
    26101 ctggctgatg cgccgcacgg gatcgctgaa cattgcgcgt aaaacaccga tcgtaatggg
    26161 catgttgctg tcgatggtga tggtgttctg caactacgtc aacgttgagt ggatgatcat
    26221 cggctttatg gcgctggcct tcttcggtaa gggcatcggg gcgctgggtt gggcagtaat
    26281 ggcagatacc gcgccaaaag agatcagcgg tctttccggt ggcctgttca acatgttcgg
    26341 taacatttct ggcatcgtca cgccaatcgc aattggttat atcgttggca cgactggctc
    26401 ctttaatggg gcgctgattt atgttggtgt tcatgcctta atcgcggtac tgagctacct
    26461 ggtgctggtg ggcgatatca agcgtatcga gctgaaacct gtcgcggggc aataagatga
    26521 cgacacaatc cagtcctgtt attactgata tgaaagtcat tccggtcgcc gggcacgata
    26581 gcatgttgct taatattggt ggtgcacata gcgcatattt cacccgcaat attgtggtac
    26641 tcaccgataa tgccgggcat accggtattg gtgaagcgcc gggcggagag gtgatttatc
    26701 agacgcttgt cgatgctatt ccgatggtgc tgggccagga agttgcgcgc ctgaataaag
    26761 tggttcagca ggtgcatata ggtaatcagg cggccgattt tgataccttc ggcaaaggtg
    26821 cctggacttt tgaattgcgc gttaatgccg tggcggcgct ggaagccgcc ttgcttgacc
    26881 tgctaggtaa ggcgttgaat gttccggtct gcgaactgtt agggccaggc aagcaacgcg
    26941 atgctattac cgtcctcggt tatctgtttt atatcggtga tcggaccaaa accgatcttc
    27001 cttatctgga aaatacgccg ggcaaccatg agtggtatca gttgcgccat cagaaagcga
    27061 tgaacagcga agccgttgtg cgtctggcgg aagcctcaca ggatcgctat ggctttaaag
    27121 atttcaaact taagggcgga gtgttacctg gcgagcaaga aatcgacact gttcgtgcat
    27181 tgaagaaacg cttcccggat gcgcggatta ccgttgatcc caacggtgca tggctgcttg
    27241 atgaagccat ttctttgtgc aaagggctga atgatgttct tacctatgcc gaagatccgt
    27301 gcggcgctga gcagggtttc tccggtcgtg aagtgatggc ggagtttcga cgggcgaccg
    27361 gcttgcccgt cgcgactaac atgatcgcca ccaactggcg cgaaatgggc catgcggtga
    27421 tgctcaatgc ggtagatatt ccacttgccg atccgcactt ctggacgctt tccggtgcag
    27481 tccgcgtggc acagctttgc gacgactggg ggctaacctg gggctgccat tctaataacc
    27541 atttcgatat ttctctggcg atgtttaccc atgtgggcgc ggcggcaccg ggtaatccta
    27601 ccgctatcga tacccactgg atttggcagg agggcgattg tcgcctgacc aaaaatccgc
    27661 tggagattaa aaacggaaaa attgccgttc ctgatgcgcc cggtctgggc gtggaactgg
    27721 actgggaaca ggtacaaaag gcacatgagg cctataaacg tctgcctggc ggtgcgcgta
    27781 acgacgcagg tccgatgcag tatctgatcc ccggctggac ctttgaccgt aaacgtcccg
    27841 ttttcggccg tcactgattc tgaataagga cataaatatg agttctcaat ttacgacgcc
    27901 tgttgttact gaaatgcagg tcatcccggt ggcgggtcat gacagtatgc tgatgaatct
    27961 gagtggtgca cacgcaccgt tcttcacgcg taatattgtg attatcaaag ataattccgg
    28021 tcacaccggc gtaggcgaaa ttcccggcgg cgagaaaatc cgtaaaacgc tggaagatgc
    28081 gattccgctg gtggtgggta gaacgctggg tgaatacaaa aacgttctga cgctggtgcg
    28141 taatacattt gccgatcgtg atgccggcgg gcgcggtttg cagacatttg atttgcgtac
    28201 cactattcat gtagttaccg ggatagaagc ggcaatgctg gatctgctgg ggcagcatct
    28261 gggggtaaac gtggcatcgc tgctgggcga tggtcaacat cgtagcgaag tcgaaatgct
    28321 cggttatctg ttcttcgtcg gtgatcgcaa agccacaccg ctgccgtatc aaagccagcc
    28381 ggatgactca tgcgactggt atcgcctgcg tcatgaagaa gcgatgacgc cggatgcggt
    28441 ggtgcgcctg gcggaagcgg cgtatgaaaa atatggcttc aacgatttca aactgaaagg
    28501 cggtgtactg gccggggaag aagaggccga gtctattgtg gcactggcgc aacgcttccc
    28561 gcaggcgcgt attacgctcg atcctaacgg tgcctggtcg ctgaacgaag cgattaaaat
    28621 tggtaaatac ctgaaagggt cgctggctta tgcagaagat ccgtgtggtg cggagcaagg
    28681 tttctccggg cgtgaagtga tggcagagtt ccgtcgcgca actggcctgc cgaccgcaac
    28741 caatatgatc gccaccgact ggcggcagat gggccatacg ctctccctgc aatccgttga
    28801 tatcccgctg gcggatccgc atttctggac gatgcaaggt tcggtacgtg tggcgcaaat
    28861 gtgccatgaa tttggcctga cctggggttc acactcaaac aaccacttcg atatttccct
    28921 ggcgatgttt acccatgttg ccgccgctgc accgggtaaa attactgcta ttgatacgca
    28981 ctggatctgg caggaaggta atcagcgcct gaccaaagaa ccgtttgaga tcaaaggcgg
    29041 tctggttcag gtgccggaaa aaccggggct gggtgtagaa atcgatatgg atcaagtgat
    29101 gaaagcccat gagctgtatc agaaacacgg gcttggcgcg cgtgacgatg cgatgggaat
    29161 gcagtatctg attcctggct ggacgttcga taacaagcgc ccgtgcatgg tgcgttaaga
    29221 cagaaaacca aaaccggctc tatctggagc cggcttgaga ctgatgctaa acgcaaaata
    29281 gcctgttgtg ctatgtatca ggtctacatc atttcttaat tcattgaatt taattggttt
    29341 tgtaggccgg ataaggcgtt aacgccgcat ccggcataaa cacaggcact ttgtcaccaa
    29401 tctgaaacca gcgtcataaa aagccgattg ctactcgaca agacgtccgt tacccgagaa
    29461 ttttgctggc ttcgcgcgct acattatcca tctcgtccaa cagttccagc agctccggct
    29521 ccaaatcttc ttctttagta ccgctacgta gctgttgttc gatcagttgg cagagattct
    29581 tcatacgcgg cacaccgcta tagccgcaac tgccatgcag tttatgaatc aaatcaacca
    29641 ggccttccgg gttttctcca accagctgtt cctcaacttt gttgcgaact tcaggcagga
    29701 aatcgagtaa catttgcagc atatcgcgcg ctaaatcggt ttttcctgct gcctggcgta
    29761 gtgccagttg ccagtcgagg gtcgcattcg ggttcaccac aatttcgttg acttcgggcg
    29821 tcacgacgcg agaggaaata ccgctgccag gcttgtagcg caacaacaaa ttatgcaatc
    29881 gctcttcttc aatcggtttc gccagataat cgctcatccc tgcgccaagc agcttctctt
    29941 tttgcccggc cattgcatgc gccgttaccg cgataaccgg cgtttgttgc tgatggggga
    30001 gctggtggat gagctcgcag gcccgaatgc catccatgtc aggcatttga atatccatta
    30061 agatcaaatc gaacggcatc tgtttcgccc gttcaaccgc ctgatgcccg ctatcgcaaa
    30121 gttccacatg ttgcaccata tcttccagca atgcgccgat aagtttcagg ttagcggggt
    30181 tgtcatcaac tgccatgact gtcattgcca gcttgctttc atcggttaca ggcaaaagcg
    30241 tgttttgttt gtgatgacaa aattccgtca gggcaggcaa caggcgcgta ggtgttaatg
    30301 gtttcagcag acacgcgccg ataccatctt gcttgagttt ttctgcattg acttgtgcat
    30361 ggcaaggaag tgccagcatc aggaaatcgg tcatcgatac cgctttcgct aatcgctcat
    30421 gttgcattgt tagcggctcg cggaaggtca ccgcgatgcc taacaacatc atgtcgtaat
    30481 gcgcgggagg cagcgcggag aacgttgggc tataaaccac ttccagcggc gtttcactta
    30541 aaatatccag cgtgcattgc gctgctgcgg agtttggttc gacataggcc aggcgcttac
    30601 cggcgaggca ctgggtggat ggcccttcga taataatgtt cgggttcaga tcgagattaa
    30661 tgtggaacca gaaagttgaa ccgcgattcg gctggctatg gaacgaaata tcgccgccca
    30721 tttcattaac cagtttttgt gtaatcacca gccccagacc ggtgccacca tgacggcggg
    30781 aaatactggc atcagcctgt cggaaggcct ggaataagcg cgattgatcg cgttcaggaa
    30841 taccaatgcc ggtatcccga atctgcactt caatctgcac tttggtatta ctcagcgcac
    30901 gtttttctac cagaatatca atgttgccat tctcggtgaa tttaattgca ttccccacca
    30961 ggttagtgat gatttgctgt aatcgcagtg ggtcgccgat cacgttatca ggcacgtcgc
    31021 ttttaatatt gagcgtcagt tccaaccctt tatcgtgaga agaatgtgcc agcagagtaa
    31081 cgacttcatc cagcgtgctg cgtagtggga atggaatact ttccagaatc agcttacctg
    31141 cttccagttt cgagaagtcg agaacatcat taataattgc cagcaaatta tttgccgaac
    31201 gttcaatcgt attcaggtga tcgcgctgcg ttggtgttaa ttctgttttc agcgtcaggc
    31261 gggtaaagcc aataacacca ttcagtggtg tacgcagctc gtgtgacata tttgccagaa
    31321 actcggattt aatacgcgcc gcttcctggg cgcgcttttt cgccagatct aactcaacgt
    31381 tctgaatttc catctgctcc agcgtttcac gcagatcgga cgtcgcctgg tcgatattgt
    31441 gctgcatctc ttcgtgataa gcagccagcg acattgccat cgagttgata ccgtttttca
    31501 gcatatccag ctcgccgagc ataaatcctt ccacccggct gtcgagttgc ccgcgacgga
    31561 tgcggtcgac ggtattcacc atgttgcgaa tcggaccggt tacatcgcgc attaagcgcc
    31621 agccaaaaat aagcgcaata ccgatacaaa acagcatcat cacgctggaa ataaagatct
    31681 ctttatattg ctgcaagcga accgatttaa gatccagctc cagcgcaata tatcccaaca
    31741 tattttgact atttttggca tcgctactgg gcgattcgtc gggggagtaa ctctcagaaa
    31801 taatcggcgt gcggaggatc ataatatcgc cgtcacgagt gacagtgagc tggcgaggaa
    31861 acggcacgtt gctgccgagc tgcattgatg agggatcaag atgaaaattg gaggtgacaa
    31921 agagtcggtt attttcatca taaaccgaaa tcgcgcgaac aatatcggaa tggcgacgat
    31981 gcagtacgct tattaactga ccgatagatt cgcgattttg caggctcatg ccatattcag
    32041 tagaaactgc aagcggctca ataatgctgg caccggcatc ttccagttga cgctgcaagt
    32101 cgttatagcg atgcacgacg aaaaagatac tcagcaataa accaataagg acggtcggtg
    32161 ccaggatcag aatcatcatg cgtgcgcgca ggctgtagtt ggtcatggag ttccgttatg
    32221 ggacaattaa ggtcacactg ttaaattgag aaagtctcgc caatggcgca attctactct
    32281 gcaaaacgac gcacgacgac gcgtcagatc ataaccgttt cagtcaacga cctcgactct
    32341 tttggtcagg gcgtggcgcg acataacggc aaaacgttat ttatccccgg attattgccg
    32401 caggaaaacg cggaagttgc tgttactgaa gataaaaaac agtatgcccg cgctaaagtc
    32461 gtacgccggt taagcgatag cccggaacgc gaaacaccac gctgtcctca ttttggcgta
    32521 tgcggcggct gtcagcaaca acacgccagc gtggatttac agcagcgaag caaaagtgcg
    32581 gcactcgccc gattaatgaa acacgatgtc tctgaagtga tcgccgatgt tccctggggc
    32641 tatcgccgcc gcgcgcgttt aagtctgaac tacttaccga aaacacagca acttcagatg
    32701 gggtttcgca aagcgggctc cagtgacatt gtcgacgtta aacaatgccc cattttagtg
    32761 ccccaacttg aagcattgct gcccaaagtc agggcatgcc tgggcagctt acaagttatg
    32821 cgccatcttg gtcatgttga actggtacag gcaaccagcg gcacgctgat gattttgcgc
    32881 cataccgcac cgctaagttc ggcagatcgc gaaaaactgg aacgcttttc gcattctgaa
    32941 ggcctggatc tgtatctcgc ccccgatagt gagatactcg aaaccgtctc tggtgagatg
    33001 ccctggtatg actcaaacgg gttgcgctta acttttagcc cgcgcgattt tattcaggtc
    33061 aatgcgggtg tgaaccaaaa aatggtagcg cgtgcgttgg aatggctgga tgtgcaacct
    33121 gaagatcgcg tactggatct gttctgcggt atgggcaact ttacactgcc attggcgaaa
    33181 caagcagcca gtgtggtcgg tgtagaaggt gttccggcgc tggtggaaaa aggccagcag
    33241 aatgcgcgtc ttaatggttt acagaatgtg acgttttatc acgaaaatct tgaagaagat
    33301 gtcacaaagc agccgtgggc gaaaaacggc ttcgataaag tgttgctgga cccggcgcga
    33361 gcaggtgccg caggtgttat gcagcaaatt ataaaactgg aacctattcg tatagtttat
    33421 gtatcctgta accctgcaac gctggctcgg gatagcgaag cgttattaaa agcaggatat
    33481 accattgcgc gactggcgat gctggatatg ttcccacaca cgggacatct ggaatcgatg
    33541 gtacttttct cgcgcgttaa atagttacga tttgccgatt tcggcaggtc tggtccctaa
    33601 aggagaggac aatggttgcg gtaagaagtg cacatatcaa taaggctggt gaatttgatc
    33661 cggaaaaatg gatcgcaagt ctgggtatta ccagccagaa gtcgtgtgag tgcttagccg
    33721 aaacctgggc gtattgtctg caacagacgc aggggcatcc ggatgccagt ctgttattgt
    33781 ggcgtggtgt tgagatggtg gagatcctct cgacattaag tatggacatt gacacgctgc
    33841 gggcggcgct gctgttccct ctggctgatg ccaacgtagt cagcgaagat gtgctgcgtg
    33901 agagcgtcgg taagtcggtc gttaacctta ttcacggcgt gcgtgatatg gcggcgatcc
    33961 gccagctgaa agcgacgcac actgattctg tttcctccga acaggtcgat aacgttcgcc
    34021 ggatgttatt ggcgatggtc gatgattttc gctgcgtagt catcaaactg gcggagcgta
    34081 ttgctcatct gcgcgaagta aaagatgcgc cggaagatga acgtgtactg gcggcaaaag
    34141 agtgtaccaa catctacgca ccgctggcta accgtctcgg aatcggacaa ctgaaatggg
    34201 aactggaaga ttactgcttc cgttacctcc atccaaccga atacaaacga attgccaaac
    34261 tgctgcatga acggcgtctc gaccgcgaac actacatcga agagttcgtt ggtcatctgc
    34321 gcgctgagat gaaagctgaa ggcgttaaag ctgaagtgta tggtcgtccg aaacacatct
    34381 acagtatctg gcgcaaaatg cagaaaaaga acctcgcctt cgatgagctg tttgatgtgc
    34441 gtgcggtacg tattgtcgcc gagcgtttac aggattgtta tgccgcactg gggatagtgc
    34501 acactcacta tcgccacctg ccggatgagt ttgacgatta tgtcgctaac ccgaaaccaa
    34561 acggttatca gtctattcat accgtggttc tggggccagg tggcaaaacc gttgagatcc
    34621 aaatccgcac caaacagatg catgaagatg cagagttggg tgttgctgcg cactggaaat
    34681 ataaagaggg cgcggcagct ggcggcgcac gttcgggaca tgaagaccgg attgcctggc
    34741 tgcgtaaact gattgcgtgg caggaagaga tggctgattc cggcgaaatg ctcgacgaag
    34801 tacgcagcca ggtctttgac gaccgggtgt atgtctttac gccgaaaggt gatgtcgttg
    34861 atttgcctgc gggatcaacg ccgctggact tcgcttacca catccacagt gatgtcggac
    34921 accgctgcat cggggcaaaa attggcgggc gcattgtgcc gttcacctac cagctgcaga
    34981 tgggcgacca gattgaaatt atcacccaga aacagccgaa ccccagccgt gactggttaa
    35041 acccaaacct cggttacgtc acaaccagcc gtgggcgttc gaaaattcac gcctggttcc
    35101 gtaaacagga ccgtgacaaa aacattctgg ctgggcggca aatccttgac gacgagctgg
    35161 aacatctggg gatcagcctg aaagaagcag aaaaacatct gctgccgcgt tacaacttta
    35221 atgatgtcga cgagttgctg gcggcgattg gtggcgggga tatccgtctc aatcagatgg
    35281 tgaacttcct gcaatcgcaa tttaataagc cgagtgccga agagcaggac gccgccgcgc
    35341 tgaagcaact tcagcaaaaa agctacacgc cgcaaaaccg cagtaaagat aacggtcgcg
    35401 tggtggtcga aggtgttggc aacctgatgc accacatcgc gcgctgctgc cagccgattc
    35461 ctggagatga gattgtcggc ttcattaccc aggggcgcgg tatttcagta caccgcgccg
    35521 attgcgaaca actggcggaa ctgcgctccc atgcgccaga acgcattgtt gacgcggtat
    35581 ggggtgagag ctactccgcc ggatattcgc tggtggtccg tgtggtggct aatgatcgta
    35641 gtgggttgtt acgtgatatc acgaccattc tcgccaacga gaaggtgaac gtgcttggcg
    35701 ttgccagccg tagcgacacc aaacagcaac tggcgaccat cgacatgacc attgagattt
    35761 acaacctgca agtgctgggg cgcgtgctgg gtaaactcaa ccaggtgccg gatgttatcg
    35821 acgcgcgtcg gttgcacggg agttaggccg aaatttgctc gtatctacaa tgtagattga
    35881 tatatactgt atctacatat gatagcggtt tgaggaaagg gttatgatcc acagtagcgt
    35941 aaagcgttgg ggaaattcac cggcggtgcg gatcccggct acgttaatgc aggcgctcaa
    36001 tctgaatatt gatgatgaag tgaagattga cctggtggat ggcaaattaa ttattgagcc
    36061 agtgcgtaaa gagcccgtat ttacgcttgc tgaactggtc aacgacatca cgccggaaaa
    36121 cctccacgag aatatcgact ggggagagcc gaaagataag gaagtctggt aatggtaagc
    36181 cgatacgtac ccgatatggg cgatctgatt tgggttgatt ttgacccgac aaaaggtagc
    36241 gagcaagctg gacatcgtcc agctgttgtc ctgagtcctt tcatgtacaa caacaaaaca
    36301 ggtatgtgtc tgtgtgttcc ttatacaacg caatcaaaag gatatccgtt cgaagttgtt
    36361 ttatccggtc aggaacgtga tggcgtagcg ttagctgatc aggtaaaaag tatcgcctgg
    36421 cgggcaagag gagcaacgaa gaaaggaaca gttgccccag aggaattaca actcattaaa
    36481 gccaaaatta acgtactgat tgggtagtgt tactaactca cttctattct ggtcacaggt
    36541 ttccacctga cagacccgca attttcagga caattcaatg aatcaaatcg accgtttgct
    36601 cactattatg cagcgcctgc gcgatccgga aaacggctgc ccgtgggata aagagcagac
    36661 atttgccacc attgcgcctt acacccttga agaaacctac gaagtgctgg acgccatcgc
    36721 ccgtgaagat tttgacgatc ttcgcggtga actgggcgat ctgctattcc aggtggtgtt
    36781 ttacgcgcaa atggctcagg aagaagggcg ctttgacttt aatgatattt gcgctgctat
    36841 tagcgataaa ttagagcgtc gccatccgca tgtttttgct gatagttctg ccgaaaacag
    36901 tagtgaagtg cttgcccgtt gggagcaaat caaaaccgaa gagcgcgcgc agaaagcgca
    36961 gcattcggcg ctggacgata ttcctcgtag tttaccggct ttaatgcgtg cgcaaaaaat
    37021 ccagaaacgt tgcgccaacg ttggcttcga ttggacgacg cttggtccgg tagtcgataa
    37081 agtctacgaa gagatcgacg aggtgatgta cgaagcgcgg caggctgttg tcgaccaggc
    37141 taaactggag gaggaaatgg gggacctgct gtttgccacg gttaatctgg ctcgccattt
    37201 agggacgaaa gcagaaatcg cattgcaaaa agcgaacgaa aaattcgagc gtcgttttcg
    37261 cgaagtggag cgtattgttg ccgcgcgtgg actggaaatg acaggtgttg acctcgaaac
    37321 aatggaagaa gtctggcaac aggtaaaacg gcaggaaatt gatctctaag ggaattacgc
    37381 ggtcaagcgc catttgtgtc attttttaaa tgacaagcgc ttgatttgcg tcaaaaacat
    37441 ttaccccaaa ggggctattt tctcactcct gatttcaata gtgcgctggc gaagaggagg
    37501 gataatgaaa gtttgtggca caggtcatgt tcgggtatac tgctttcccg tcctggttat
    37561 tccatcgtct tttcaaccta acttctcagg ttcagcatga caacgaacta tatttttgtg
    37621 accggcgggg tcgtatcctc tctgggtaaa ggcattgccg cagcctccct cgcagccatt
    37681 cttgaagccc gtggcctcaa tgtgaccatc atgaaactgg atccgtacat caacgtcgat
    37741 ccaggtacta tgagcccaat ccaacacggg gaagtgttcg ttactgaaga cggcgctgaa
    37801 accgacctgg acctggggca ctacgagcgt ttcattcgta ccaaaatgag ccgccgcaac
    37861 aacttcacca cgggtcgtat ctactctgac gttctgcgta aagaacgccg cggtgactac
    37921 ctcggcgcaa ccgtgcaggt tattccgcac atcactaacg caatcaaaga gcgcgtgctg
    37981 gaaggtggcg aaggtcatga cgtagtactg gtagaaatcg gcggtacagt aggtgatatc
    38041 gaatccttgc cgttcctcga agcgattcgc cagatggctg ttgaaattgg ccgtgagcac
    38101 actctgttta tgcacctgac gctggtgccg tacatggcag cgtctggtga agtcaaaacc
    38161 aaaccgactc agcactctgt aaaagagctg ctctccatcg gtatccagcc tgacatcctg
    38221 atttgtcgtt cagatcgcgc tgttccggcg aacgaacgtg cgaagattgc attgttctgt
    38281 aatgttccgg aaaaagcggt tatttctctg aaagacgtcg attccatcta taaaattccg
    38341 ggcctgttga aatctcaggg gctggacgat tatatttgta aacgattcag cttaaactgc
    38401 ccggaagcga atctgtccga atgggaacag gttatcttcg aagaagcgaa cccggtaagt
    38461 gaagtcacca tcggtatggt cggcaagtac attgaactgc cggatgctta taaatcagtg
    38521 atcgaagcac tgaaacacgg tgggctgaag aatcgtgtca gcgtcaacat caaactgatc
    38581 gattcacaag atgttgaaac gcgcggcgtt gaaatcctta aaggtctgga cgcaatccta
    38641 gtacctggcg gttttggcta tcgtggcgta gaaggcatga ttacgaccgc gcgttttgcg
    38701 cgtgagaaca atattcctta tctgggcatt tgcctgggta tgcaggtggc gttaattgat
    38761 tacgctcgcc atgttgccaa catggagaac gccaactcta cggaatttgt gccagactgt
    38821 aagtacccgg ttgtggcgct gattaccgag tggcgcgatg aaaacggcaa cgttgaagtt
    38881 cgtagcgaga agagcgatct cggcggtacc atgcgtctcg gcgcacagca gtgccagttg
    38941 gttgacgata gcctggttcg ccagctgtac aatgcgccga caattgttga gcgtcatcgt
    39001 caccgttacg aagtcaacaa catgctgttg aaacagattg aagatgcagg tctgcgcgtt
    39061 gcgggccgtt ccggggatga tcagttggtc gagatcatcg aagttccgaa tcacccgtgg
    39121 ttcgtggctt gccagttcca tccggagttt acttctactc cacgtgatgg tcacccgctg
    39181 tttgctggct ttgtaaaagc cgccagcgag ttccagaagc gtcaggcgaa gtaagtaaaa
    39241 aagttagaac ggcaacgcgt accctgggta cgcgttgttt gtctggagtt tcagtttaac
    39301 tagtgacttg aggaaaacct aatgtccaaa atcgtaaaaa tcatcggtcg tgaaatcatc
    39361 gactcccgtg gtaacccgac tgttgaagcc gaagtacatc tggagggtgg tttcgtcggt
    39421 atggcagctg ctccgtcagg tgcttctact ggttcccgtg aagctctgga actgcgcgat
    39481 ggcgacaaat cccgtttcct gggtaaaggc gtaaccaaag ctgttgctgc ggtaaacggc
    39541 ccgatcgctc aggcgctgat tggcaaagat gctaaagatc aggctggcat tgacaagatc
    39601 atgatcgacc tggacggcac cgaaaacaaa tccaaattcg gcgcgaacgc aatcctggct
    39661 gtatctctgg ctaacgccaa agctgctgca gctgctaaag gtatgccgct gtacgagcac
    39721 atcgctgaac tgaacggtac tccgggcaaa tactctatgc cggttccgat gatgaacatc
    39781 atcaacggtg gtgagcacgc tgacaacaac gttgatatcc aggaattcat gattcagccg
    39841 gttggcgcga aaactgtgaa agaagccatc cgcatgggtt ctgaagtttt ccatcacctg
    39901 gcaaaagttc tgaaagcgaa aggcatgaac actgctgttg gtgacgaagg tggctatgcg
    39961 ccgaacctgg gttccaacgc tgaagctctg gctgttatcg ctgaagctgt taaagctgct
    40021 ggttatgaac tgggcaaaga catcactctg gcgatggact gcgcagcttc tgaattctac
    40081 aaagatggta aatacgttct ggctggcgaa ggcaacaaag cgttcacctc tgaagaattc
    40141 actcacttcc tggaagaact gaccaaacag tacccgatcg tttctatcga agacggtctg
    40201 gacgaatctg actgggacgg tttcgcatac cagaccaaag ttctgggcga caaaatccag
    40261 ctggttggtg acgacctgtt cgtaaccaac accaagatcc tgaaagaagg tatcgaaaaa
    40321 ggtatcgcta actccatcct gatcaaattc aaccagatcg gttctctgac cgaaactctg
    40381 gctgcaatca agatggcgaa agatgctggc tacactgcag ttatctctca ccgttctggc
    40441 gaaactgaag acgctaccat cgctgacctg gctgttggta ctgctgcagg ccagatcaaa
    40501 actggttcta tgagccgttc tgaccgtgtt gctaaataca accagctgat tcgtatcgaa
    40561 gaagctctgg gcgaaaaagc accgtacaac ggtcgtaaag agatcaaagg ccaggcataa
    40621 gactgacttt atctgattta aaaatgctag cctccgggct ggcatttttt atggttgagt
    40681 taccagcggc cggatgcgcc gccacctcca gaagaaccgc caccgccgct aaatccgccg
    40741 ccacttgagc ctccaccgga accgctgttg tctgaatgat aacttttacc tgatgctctt
    40801 ttctgaagta cacataaaca ggtaaagacc ataaatatgg tgaaagtaaa aaagaaggaa
    40861 aatacagcaa tggaaaaaat tttatcggag aacaggaata aatctaaaag atagattgct
    40921 gaaatataga ctccactttt acatgcgcga caaaaattac tgccttgatg aaaaaaccag
    40981 aaagggaaga acatcactgc aaatacccaa aaaatagcaa agtaataatg atcactggaa
    41041 gacgctgatt cactttctga aggattagtc ggatattggt gttgtgaagt gagttggtta
    41101 ttcaaagcgt ttatagctag ctctaatccc tgagcaaatt tttgttgttt aaatgcgggt
    41161 atcatgttgc tacggatgat atctccagcc agagcatcgg ttaccttttc ttccagccca
    41221 taacctacct ggatgcggac agtacgatct gaccaggcaa caatgatcag tatcccatca
    41281 ttacgtttgg catctcctaa acgccaattg tcaaaaactc ttgtagcata ttgttcaatt
    41341 gtctcgtctt tggttgtggg aacaattaat acagcaattt gcacttgggt attattgaaa
    41401 atagagtcta acttgtgcgt cagttctttc ttttcttgtg agtttaagct actcgtaaga
    41461 tccgtaactt gttgctgtaa ttgtgggaca atagttggtt gggctgcaac aaaggaacac
    41521 acgaaagtga acattagtat gaaatagcgc attataaatt tctcattaaa tctggataaa
    41581 taaattgacg gcggcatcgg taattttaga ttcgttatca ttaaaaacta acacagaaaa
    41641 aataaatata attttggtga atgttttgac atgattaaat ccattttctt tattttcttc
    41701 tgacataata cgttactccc tttaattatt aacgatatgt ttcgcatttt tctttaaatc
    41761 tgcctgtaac agaatgttaa taagacatga ttgttgtggc aggatctgca ataattaaca
    41821 attatccact tcacagagaa tgctatgcag tacccgatta acgagatgtt ccagaccctg
    41881 caaggtgagg gttactttac cggcgtcccc gccattttta ttcgtttaca gggatgcccg
    41941 gttggctgtg cctggtgcga caccaaacac acctgggaaa agcttgagga tcgggaagtc
    42001 tcccttttca gcattctggc gaagaccaaa gagagtgata agtggggggc ggcgagcagt
    42061 gaagatttgc tggctgtcat tggtcggcag ggatataccg cgcggcatgt ggtgattacg
    42121 ggtggtgagc cttgcattca tgatttgctg ccactgactg acctgctcga aaagaacggt
    42181 tttagctgcc agatcgaaac cagtggaact catgaagtac gctgcacacc gaatacctgg
    42241 gttaccgtat cgccaaagct gaacatgcgc ggcggctatg acgtgttgtc acaggcactg
    42301 gagcgagcca acgaaatcaa gcatccagtg gggcgcgtac gcgatattga agcactggat
    42361 gaactattgg caacgctgac cgatgataaa ccgcgagtca ttgcactgca gccgattagc
    42421 caaaaggatg atgccacacg tttgtgcatt gaaacctgca ttgcgcgtaa ttggcgtttg
    42481 tcgatgcaaa cacataaata tctaaatatt gcctgattaa acatttataa gcgttataaa
    42541 tgggtggaac ctgtaaagac ttctactcat ttatattgtt tgtcgcctct gaaaactcct
    42601 ccattttacc catccagggc taatcattag cattctctac aaattctgtg gcattaattt
    42661 ttcgctggag tgaaaattat tgcggtaaag tttggtagat tttaatttgt atagagttat
    42721 tttaaatatt taccttttta atcaatgaat taagtactct ttaacataat ggatgtgttg
    42781 tttgtgtgat actgtaaagt tggtagattg tgactggctt aaaaaatcat taattaataa
    42841 taggttatgt ttacagagtt ccccgcgcca gcggggataa accgccgctt aaccggacct
    42901 catcacaaaa cacgcggagt tccccgcgcc agcggggata aaccaccata taacccgtta
    42961 tctctttctc aagtttttat attagcagta cttgtaataa gcaacatatc cacgtaacac
    43021 ctcatgttca aaatagttct ccatgccaga ggttcacaat tatcgataca aaaaattaaa
    43081 tttaatcaaa gtgttatttg tatgattctt aaatcgttaa
//
LOCUS       DAZGAN020000028        42935 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGAN020000028 DAZGAN020000000
VERSION     DAZGAN020000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 42935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.028"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(169..844)
                     /locus_tag="ACHKYO_RS08855"
                     /old_locus_tag="pgaptmp_001771"
                     /pseudo
     CDS             complement(169..844)
                     /locus_tag="ACHKYO_RS08855"
                     /old_locus_tag="pgaptmp_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(989..1597)
                     /gene="yiiX"
                     /locus_tag="ACHKYO_RS08860"
                     /old_locus_tag="pgaptmp_001772"
     CDS             complement(989..1597)
                     /gene="yiiX"
                     /locus_tag="ACHKYO_RS08860"
                     /old_locus_tag="pgaptmp_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HGU4428630.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1781..2098)
                     /gene="metJ"
                     /locus_tag="ACHKYO_RS08865"
                     /old_locus_tag="pgaptmp_001773"
     CDS             complement(1781..2098)
                     /gene="metJ"
                     /locus_tag="ACHKYO_RS08865"
                     /old_locus_tag="pgaptmp_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HGU4428631.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2375..3535
                     /gene="metB"
                     /locus_tag="ACHKYO_RS08870"
                     /old_locus_tag="pgaptmp_001774"
     CDS             2375..3535
                     /gene="metB"
                     /locus_tag="ACHKYO_RS08870"
                     /old_locus_tag="pgaptmp_001774"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGU4428632.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3538..5970
                     /gene="metL"
                     /locus_tag="ACHKYO_RS08875"
                     /old_locus_tag="pgaptmp_001775"
     CDS             3538..5970
                     /gene="metL"
                     /locus_tag="ACHKYO_RS08875"
                     /old_locus_tag="pgaptmp_001775"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HGU4428633.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6109..6243)
                     /locus_tag="ACHKYO_RS08880"
                     /old_locus_tag="pgaptmp_001776"
     CDS             complement(6109..6243)
                     /locus_tag="ACHKYO_RS08880"
                     /old_locus_tag="pgaptmp_001776"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4428634.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6319..7209
                     /gene="metF"
                     /locus_tag="ACHKYO_RS08885"
                     /old_locus_tag="pgaptmp_001777"
     CDS             6319..7209
                     /gene="metF"
                     /locus_tag="ACHKYO_RS08885"
                     /old_locus_tag="pgaptmp_001777"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HGU4428635.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7538..9718
                     /gene="katG"
                     /locus_tag="ACHKYO_RS08890"
                     /old_locus_tag="pgaptmp_001778"
     CDS             7538..9718
                     /gene="katG"
                     /locus_tag="ACHKYO_RS08890"
                     /old_locus_tag="pgaptmp_001778"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HGU4428636.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9812..10717
                     /gene="yijE"
                     /locus_tag="ACHKYO_RS08895"
                     /old_locus_tag="pgaptmp_001779"
     CDS             9812..10717
                     /gene="yijE"
                     /locus_tag="ACHKYO_RS08895"
                     /old_locus_tag="pgaptmp_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HGU4428637.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(10744..11361)
                     /gene="yijF"
                     /locus_tag="ACHKYO_RS08900"
                     /old_locus_tag="pgaptmp_001780"
     CDS             complement(10744..11361)
                     /gene="yijF"
                     /locus_tag="ACHKYO_RS08900"
                     /old_locus_tag="pgaptmp_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HGU4428638.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11636..12739)
                     /gene="gldA"
                     /locus_tag="ACHKYO_RS08905"
                     /old_locus_tag="pgaptmp_001781"
     CDS             complement(11636..12739)
                     /gene="gldA"
                     /locus_tag="ACHKYO_RS08905"
                     /old_locus_tag="pgaptmp_001781"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HGU4428639.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALEHVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12750..13412)
                     /gene="fsa"
                     /locus_tag="ACHKYO_RS08910"
                     /old_locus_tag="pgaptmp_001782"
     CDS             complement(12750..13412)
                     /gene="fsa"
                     /locus_tag="ACHKYO_RS08910"
                     /old_locus_tag="pgaptmp_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU4428640.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13424..15925)
                     /gene="ptsP"
                     /locus_tag="ACHKYO_RS08915"
                     /old_locus_tag="pgaptmp_001783"
     CDS             complement(13424..15925)
                     /gene="ptsP"
                     /locus_tag="ACHKYO_RS08915"
                     /old_locus_tag="pgaptmp_001783"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4428641.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16234..17313
                     /gene="frwC"
                     /locus_tag="ACHKYO_RS08920"
                     /old_locus_tag="pgaptmp_001784"
     CDS             16234..17313
                     /gene="frwC"
                     /locus_tag="ACHKYO_RS08920"
                     /old_locus_tag="pgaptmp_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HGU4428642.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17328..17648
                     /gene="frwB"
                     /locus_tag="ACHKYO_RS08925"
                     /old_locus_tag="pgaptmp_001785"
     CDS             17328..17648
                     /gene="frwB"
                     /locus_tag="ACHKYO_RS08925"
                     /old_locus_tag="pgaptmp_001785"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU4428643.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17699..19996
                     /gene="pflD"
                     /locus_tag="ACHKYO_RS08930"
                     /old_locus_tag="pgaptmp_001786"
     CDS             17699..19996
                     /gene="pflD"
                     /locus_tag="ACHKYO_RS08930"
                     /old_locus_tag="pgaptmp_001786"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU4428644.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQLFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19962..20840
                     /gene="pflC"
                     /locus_tag="ACHKYO_RS08935"
                     /old_locus_tag="pgaptmp_001787"
     CDS             19962..20840
                     /gene="pflC"
                     /locus_tag="ACHKYO_RS08935"
                     /old_locus_tag="pgaptmp_001787"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HGU4428645.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVNMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20842..21183
                     /gene="frwD"
                     /locus_tag="ACHKYO_RS08940"
                     /old_locus_tag="pgaptmp_001788"
     CDS             20842..21183
                     /gene="frwD"
                     /locus_tag="ACHKYO_RS08940"
                     /old_locus_tag="pgaptmp_001788"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU4428646.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21170..22021)
                     /gene="yijO"
                     /locus_tag="ACHKYO_RS08945"
                     /old_locus_tag="pgaptmp_001789"
     CDS             complement(21170..22021)
                     /gene="yijO"
                     /locus_tag="ACHKYO_RS08945"
                     /old_locus_tag="pgaptmp_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4428647.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22170..23903)
                     /gene="eptC"
                     /locus_tag="ACHKYO_RS08950"
                     /old_locus_tag="pgaptmp_001790"
     CDS             complement(22170..23903)
                     /gene="eptC"
                     /locus_tag="ACHKYO_RS08950"
                     /old_locus_tag="pgaptmp_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU4428648.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24086..26737)
                     /gene="ppc"
                     /locus_tag="ACHKYO_RS08955"
                     /old_locus_tag="pgaptmp_001791"
     CDS             complement(24086..26737)
                     /gene="ppc"
                     /locus_tag="ACHKYO_RS08955"
                     /old_locus_tag="pgaptmp_001791"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGU4428649.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27089..28240)
                     /gene="argE"
                     /locus_tag="ACHKYO_RS08960"
                     /old_locus_tag="pgaptmp_001792"
     CDS             complement(27089..28240)
                     /gene="argE"
                     /locus_tag="ACHKYO_RS08960"
                     /old_locus_tag="pgaptmp_001792"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HGU4428650.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28394..29398
                     /gene="argC"
                     /locus_tag="ACHKYO_RS08965"
                     /old_locus_tag="pgaptmp_001793"
     CDS             28394..29398
                     /gene="argC"
                     /locus_tag="ACHKYO_RS08965"
                     /old_locus_tag="pgaptmp_001793"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HGU4428651.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29406..30182
                     /gene="argB"
                     /locus_tag="ACHKYO_RS08970"
                     /old_locus_tag="pgaptmp_001794"
     CDS             29406..30182
                     /gene="argB"
                     /locus_tag="ACHKYO_RS08970"
                     /old_locus_tag="pgaptmp_001794"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HGU4428652.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30243..31616
                     /gene="argH"
                     /locus_tag="ACHKYO_RS08975"
                     /old_locus_tag="pgaptmp_001795"
     CDS             30243..31616
                     /gene="argH"
                     /locus_tag="ACHKYO_RS08975"
                     /old_locus_tag="pgaptmp_001795"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGU4428653.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            32115..33329
                     /gene="manD"
                     /locus_tag="ACHKYO_RS08980"
                     /old_locus_tag="pgaptmp_001796"
     CDS             32115..33329
                     /gene="manD"
                     /locus_tag="ACHKYO_RS08980"
                     /old_locus_tag="pgaptmp_001796"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HGU4428654.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            33396..34679
                     /locus_tag="ACHKYO_RS08985"
                     /old_locus_tag="pgaptmp_001797"
     CDS             33396..34679
                     /locus_tag="ACHKYO_RS08985"
                     /old_locus_tag="pgaptmp_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4428655.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            34931..35848
                     /gene="oxyR"
                     /locus_tag="ACHKYO_RS08990"
                     /old_locus_tag="pgaptmp_001798"
     CDS             34931..35848
                     /gene="oxyR"
                     /locus_tag="ACHKYO_RS08990"
                     /old_locus_tag="pgaptmp_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HGU4428656.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(35831..37231)
                     /gene="sthA"
                     /locus_tag="ACHKYO_RS08995"
                     /old_locus_tag="pgaptmp_001799"
     CDS             complement(35831..37231)
                     /gene="sthA"
                     /locus_tag="ACHKYO_RS08995"
                     /old_locus_tag="pgaptmp_001799"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HGU4428657.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            37552..38202
                     /gene="fabR"
                     /locus_tag="ACHKYO_RS09000"
                     /old_locus_tag="pgaptmp_001800"
     CDS             37552..38202
                     /gene="fabR"
                     /locus_tag="ACHKYO_RS09000"
                     /old_locus_tag="pgaptmp_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HGU4428658.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            38202..38561
                     /gene="yijD"
                     /locus_tag="ACHKYO_RS09005"
                     /old_locus_tag="pgaptmp_001801"
     CDS             38202..38561
                     /gene="yijD"
                     /locus_tag="ACHKYO_RS09005"
                     /old_locus_tag="pgaptmp_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HGU4428659.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(38601..39701)
                     /gene="trmA"
                     /locus_tag="ACHKYO_RS09010"
                     /old_locus_tag="pgaptmp_001802"
     CDS             complement(38601..39701)
                     /gene="trmA"
                     /locus_tag="ACHKYO_RS09010"
                     /old_locus_tag="pgaptmp_001802"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HGU4428660.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      39815..40005
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            40070..41914
                     /gene="btuB"
                     /locus_tag="ACHKYO_RS09015"
                     /old_locus_tag="pgaptmp_001803"
     CDS             40070..41914
                     /gene="btuB"
                     /locus_tag="ACHKYO_RS09015"
                     /old_locus_tag="pgaptmp_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HGU4428661.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            41859..42716
                     /gene="murI"
                     /locus_tag="ACHKYO_RS09020"
                     /old_locus_tag="pgaptmp_001804"
     CDS             41859..42716
                     /gene="murI"
                     /locus_tag="ACHKYO_RS09020"
                     /old_locus_tag="pgaptmp_001804"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGU4428662.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9777 a        10701 c        11812 g        10645 t
ORIGIN      
        1 gggcggggga atgtcatatg cctatgaata tgatgtaaaa tataatatat gtcatttgag
       61 aaaaaagagc agaatgaggt aaaaatcttt ggctgtgtat tcagtctggt aatcatccaa
      121 ggaaagctta tcagaagggt gatgactcca attagtagaa catatatttt cgataaaagc
      181 accgatgact ttttcatgaa gttctacaga acgcgagaaa cagatggttc tgcgggccag
      241 acgtttgatt cgggttctca gggtcagatt gttacgctca atgtgctgag taaaaatctt
      301 accagtcaga tgcttctcct tcggtaactc tctagcgtaa ctaccccatt cgtcagtggt
      361 caacatgccg atggtaaaag ggtaagcagc gcccgcaatt cacgacaggt ttcgtcagtc
      421 cgggggccaa aagtgtaggc cagaacacca ccagttttcg tgttgtacac gtaccagagc
      481 cagtgctgtc gcgctttact gccgacaaag ctccattgct gactaagttt gcagatgagt
      541 gccacatcag catgagcgac cggggatgac gttattcttc gtggcgagag ttttttaaag
      601 ttcggatgac tgtattaatg ccgattttca gcgtacgggc agtatcgcga accccggcac
      661 cgttgaaagt catttcagcg atttggtctt taactcccgg tttacggggc tcgtaacagt
      721 aactgagctg aaatacccgg tgacagtcac ggccgcggaa tcgatcatgt ctttttgggt
      781 tctgcccgtg acggtaaacc tgaacggact ggcaacgggg gcaatgaagg ttaactgtga
      841 ccatgaaaca accttaaaag cgagtattat atccttaatt caactaattg gaggcatcac
      901 cgtttaccgg agcgacagct tgcggaaagg tcgtgcaact acaaagcaac aaacagacta
      961 cggcgggaat tatttttttc atcatccatt accacgaaaa cagcggccac tctttggcaa
     1021 ccgtggtgag ttgtggcgcg tcgaaaacgg cttgtggtga aactaccgtt tcctcaagcg
     1081 gaatattttt accgtagcgt tctttgagct tcgcctggac cagcgggttg ctgagatcaa
     1141 actctttcaa cttctgctgt tcgcccacgc gcattcccag cgcgttctgg taaaccttcc
     1201 acaccacttc cgaacagtac tggcgatcgt cgctccatga gaagctgaaa tcatacggtt
     1261 taccgagata acgttttgcc gtttgcgcca gtttttgctg ttgttcaaca ctcagtccgc
     1321 cttcaacgcg gcgaacgacg tatttgccct tttcaccatg ggcgatccac tgcttgagcg
     1381 gggtgtattt caccgggccg accgcttcaa aaacgtaggg ctttttgttg cgtatcacca
     1441 gcataccggt gtggctgtaa tcggaatggg ttgccagttg gatcgcttta ctttgcgatg
     1501 agcgagagat ttgaaagatg atgtcgccag tttgcggctg ccaggcaaag gcaggtacag
     1561 aaaccagcag gctgaggatc agcagcctgt ttttcatagt taaacgtcca tgtataaaaa
     1621 gcggtgggtc gcagacaacg tgctcgttgt ttatgccgga tgcggcgtga acgccttatc
     1681 cggcctacaa gttcgtgcaa attcaataaa ttgcaatatg acgtaggcct gataagcgta
     1741 gcgcatcagg cggttccact ccgcgccgct cttttccgct ttagtattcc cacgtctccg
     1801 ggttaatccc catctcacgc atgatctctt ttgccgcttc cgggatttcg tcgctgcgct
     1861 ctttacgcag atcggcatca tccggcaaag gttgcccggt aaaggcatgc agaaacgctt
     1921 cgcacagcag ctcgctgttg gtagcgtgac gcaggttgtt cacctgacga cgcgtgcgtt
     1981 catcggtgag gatttttaac accttaagag gaatggaaac cgtaatcttt ttgacttgtt
     2041 cactcttctt gccgtgctca gcgtatgggc tgatatattc gccgctccat tcagccatga
     2101 gatacttaat cctcttcgtc aataaattga gaccagacca cacagttaat gtgggcactg
     2161 gccgtaaacc cgcatagttt accgtacagg cgctaccgtt acatcgggta atccacctgc
     2221 tggcgtgata atgcatataa ttttaacggc tatttgggat ttgctcaatc tatacgcaaa
     2281 gaagtttaga tgtccagatg tattgacgtc cattaccaca atgtttactc tggtgcctga
     2341 catttcacca ataaagccca gggaacatca tcacatgacg cgtaaacagg ccaccatcgc
     2401 agtgcgtagc gggttaaatg acgacgaaca gtatggttgc gttgtcccac ccatccatct
     2461 ttccagtacc tataacttta ccggatttaa tgaaccgcgc gcgcatgatt actcgcgtcg
     2521 tggcaaccca acgcgcgatg tggttcagcg agcactggca gaactggaag gcggtgctgg
     2581 tgcagtactt actaataccg gcatgtccgc gattcacctg gtaacgaccg tctttttgaa
     2641 acctggcgat ctgctggtag cgccgcacga ctgctacggc ggtagctatc gcctgttcga
     2701 cagcctggcg aaacgtggtt gctatcgcgt gttgtttgtt gatcaaggcg atgaacaggc
     2761 attacgggca gcgctggcag aaaaacccaa actggtactg gtagaaagcc caagtaatcc
     2821 attgttacgc gtcgtggata ttgcgaaaat ctgccatctg gcaagggaag tcggggcggt
     2881 gagcgtggtg gataacacct tcttaagccc ggcattacaa aatccgctgg cattaggtgc
     2941 cgatctggtg ttgcattcat gcacgaaata tctgaacggt cactcagacg tggtggccgg
     3001 cgtggtgatt gctaaagacc cggacgttgt cactgaactt gcctggtggg caaacaatat
     3061 tggcgtgacg ggcggcgcgt ttgacagcta tctgctgcta cgcgggttgc gaacgctggt
     3121 gccgcgcatg gagttggccc agcgtaacgc gcaggcgatt gtgaaatatc tgcaaaccca
     3181 gccgttggtg aaaaaactgt atcacccgtc gctgccggaa aatcaggggc atgaaattgc
     3241 cgcgcgtcag caaaaaggct ttggcgcaat gttgagtttt gaactggatg gcgatgagca
     3301 gacgctgcgt cgtttcctga gcgggctgtc gttgtttacg ctggcggaat cattaggggg
     3361 agtggaaagt ttaatctctc acgccgcaac catgacacat gcaggcatgg caccagaagc
     3421 gcgtgctgcc gccgggatct ccgagacgct gctgcgtatc tccaccggta ttgaagatgg
     3481 cgaagattta attgccgacc tggaaaatgg cttccgggct gcaaacaagg ggtaaaaatg
     3541 agtgtgattg cgcaggcagg ggcgaaaggt cgtcagctgc ataaatttgg tggcagtagt
     3601 ctggctgatg tgaagtgtta tttgcgtgtc gcgggcatta tggcggagta ctctcagcct
     3661 gacgatatga tggtggtttc cgccgccggt agcaccacta accagttgat taactggttg
     3721 aaactaagcc agaccgatcg tctctctgcg catcaggttc aacaaacgct gcgtcgctat
     3781 cagtgcgatc tgattagcgg tctgctaccc gctgaagaag ccgatagcct cattagcgct
     3841 tttgtcagcg accttgagcg cctggcggcg ctgctcgaca gcggtattaa cgacgcagtg
     3901 tatgcggaag tggtggggca cggtgaagtg tggtcggcac gcctgatgtc tgcggtactt
     3961 aatcaacaag ggctgccagc ggcctggctt gatgcccgcg agtttttacg cgctgaacgc
     4021 gccgcacaac cgcaggttga tgaagggctt tcttacccgt tgctgcaaca gctgctggtg
     4081 caacatccgg gcaaacgtct ggtggtgacc ggatttatca gccgcaacaa cgccggtgaa
     4141 acggtgctgc tggggcgtaa cggttccgac tattccgcga cacaaatcgg tgcgctggcg
     4201 ggggtttctc gcgtaaccat ctggagcgac gtcgccgggg tatacagtgc cgacccgcgt
     4261 aaagtgaaag atgcctgcct gctgccgttg ctgcgtctgg atgaggccag cgaactggcg
     4321 cgcctggcgg ctcccgttct tcacgcccgt actttacagc cggtttctgg cagcgaaatc
     4381 gacctgcaac tgcgctgtag ctacacgccg gatcaaggtt ccacgcgcat tgaacgcgtg
     4441 ctggcctccg gtactggtgc gcgtattgtc accagccacg atgatgtctg tttgattgag
     4501 tttcaggtgc ccgccagtca ggatttcaaa ctggcgcata aagagatcga ccaaatcctg
     4561 aaacgcgcgc aggtacgccc gctggcggtt ggcgtacata acgatcgcca gttgctgcaa
     4621 ttttgctaca cctcagaagt ggccgacagt gcgctgaaaa tcctcgacga agcgggatta
     4681 cctggcgaac tgcgcctgcg tcaggggctg gcgctggtgg cgatggtcgg tgcaggcgtc
     4741 acccgtaacc cgctgcattg ccaccgcttc tggcagcaac tgaaaggcca gccggtcgaa
     4801 tttacctggc agtccgatga cggcatcagc ctggtggcag tactgcgcac cggcccgacc
     4861 gaaagcctga ttcaggggct gcatcagtcc gtcttccgcg cagaaaaacg catcggcctg
     4921 gtattgttcg gtaagggcaa tatcggttcc cgttggctgg aactgttcgc ccgtgagcag
     4981 agcacgcttt cggcacgtac cggctttgag tttgtgctgg caggtgtggt ggacagccgc
     5041 cgcagcctgt tgagctatga cgggctggac gccagccgcg cgttagcctt cttcaacgat
     5101 gaagcggttg agcaggatga agagtcgttg ttcctgtgga tgcgcgccca tccgtatgat
     5161 gatttagtgg tgctggacgt taccgccagc cagcagcttg ctgatcagta tcttgatttc
     5221 gccagccacg gtttccacgt tatcagcgcc aacaaactgg cgggagccag cgacagcaat
     5281 aaatatcgcc agatccacga cgccttcgaa aaaaccgggc gtcactggct gtacaatgcc
     5341 accgtcggtg cgggcttgcc gatcaaccac accgtgcgcg atctgatcga cagcggcgat
     5401 actattttgt cgatcagcgg gatcttctcc ggcacgctct cctggctgtt cctgcaattc
     5461 gacggtagcg tgccgtttac cgagctggtg gatcaggcgt ggcagcaggg cttaaccgag
     5521 cctgatccgc gtgacgacct ctccggcaaa gacgtaatgc gcaagctggt aattctggcg
     5581 cgtgaagcgg gttacaacat tgaaccggac caggtacgtg tggaatcgct ggtgcctgct
     5641 cattgcgaag gcggcagcat cgaccatttc tttgaaaatg gcgatgaact taacgagcag
     5701 atggtgcaac ggctggaagc ggcccgcgaa atggggctgg tgctgcgcta cgtggcgcgt
     5761 ttcgatgcca acggcaaagc gcgtgtgggc gtggaggcgg tgcgtgaaga ccatccgctg
     5821 gcatcactgc tgccgtgcga taacgtcttt gccatcgaaa gccgctggta tcgcgacaac
     5881 ccgctggtga tccgtgggcc tggcgcgggg cgcgacgtca ccgccggggc gattcagtcg
     5941 gatatcaacc ggctggcaca gttgttgtaa tttcagaaat ttaataatgc ccggtactca
     6001 tgttttcggg tttatggttt ctaatgaaat atattgaatt atcataggat taggccggat
     6061 taagcgttta cgacgaatcc ggcaagaagc aataagtaca tggttagttt atatttgcag
     6121 tccggtttgc tttgcatacc ggattttctt tttcttacca tcctgaagtt ttttcatctt
     6181 ccctgatttt tcctcaccat cattggtcat ttttcggttg acgcccttcg gcttttcctt
     6241 catctttaca tctggacgtc taaacggata gatgtgcaca acacaacata taactacaag
     6301 cgattgatga ggtaaggtat gagctttttt cacgccagcc agcgggatgc cctgaatcag
     6361 agcctggcag aagtccaggg gcaaattaac gtttcgttcg agtttttccc gccgcgtacc
     6421 agtgaaatgg agcagaccct gtggaactcc atcgatcgcc ttagcagcct gaaaccgaag
     6481 tttgtatcgg tgacctatgg cgcgaactcc ggcgagcgcg accgtacgca cagcattatt
     6541 aaaggcatta aagatcgcac tggtctggaa gcggcaccgc atcttacttg cattgatgcg
     6601 acgcccgacg agctgcgcac cattgcacgc gactactgga ataacggtat tcgtcatatc
     6661 gtggcgctgc gtggcgatct gccgccggga agtggtaagc cggaaatgta tgcttctgac
     6721 ctggtgacgc tgttaaaaga agtggcagat ttcgatatct ccgtggcggc gtatccggag
     6781 gttcacccgg aagcaaaaag cgctcaggcg gatttgctca acctgaaacg caaagtcgat
     6841 gccggagcca accgcgcgat tactcagttc ttctttgatg tcgaaagcta cctgcgtttt
     6901 cgtgaccgct gcgtatcggc gggtattgat gtggaaatta ttccgggaat tttgccggtc
     6961 tcgaacttta aacaagcgaa gaaatttgcc gatatgacca acgtacgtat tccggcgtgg
     7021 atggcacaaa tgtttgacgg gctggacgac gatgccgaaa cccgcaaact ggtgggagcg
     7081 aatattgcca tggatatggt gaagatttta agccgtgaag gagtgaaaga tttccacttc
     7141 tatacgctta accgcgctga aatgagttac gcgatttgcc atacgctggg tgttcgacct
     7201 ggtttataaa tagtgtggct tttgtgaaaa tcacacagtg atcacaaatt ttaaacagag
     7261 cacaaaatgc tgcctcgaaa tgagggcggg aaaataaggt tatcagcctt gttttctccc
     7321 tcattacttg aaggatatga acctaaaacc cttttttata aagcatttgt ccgaattcgg
     7381 acataatcaa aaaagcttaa ttacgatcaa tttgatctac atctctttaa ccaacaatat
     7441 gtaagatctc aactatcgca tccgtggatt aattcaatta taacttctct ctaacgctgt
     7501 gtatcgtaac ggtaacactg tagaggggag cacattgatg agcacgtcag acgatattca
     7561 taacaccaca gccactggca aatgcccgtt ccatcagggc ggtcacgacc agagcgcggg
     7621 ggcgggcaca accactcgcg actggtggcc aaatcaactc cgtgttgacc tgttaaacca
     7681 acattctaat cgttctaacc cactgggtga ggactttgac taccgcaaag aattcagcaa
     7741 attagattac tacggcctga aaaaagatct gaaagccctg ctgacagaat ctcagccgtg
     7801 gtggcctgcc gactggggca gttacgccgg tctgtttatt cgtatggcct ggcacggcgc
     7861 gggtacttac cgttcaatcg atggacgcgg cggcgcgggt cgtggtcagc aacgttttgc
     7921 accgctgaac tcttggccgg ataacgtaag cctcgataaa gcgcgtcgcc tgttgtggcc
     7981 aatcaaacag aaatatggtc agaaaatctc ctgggccgac ctgtttatcc tcgcaggtaa
     8041 cgtggcgctg gaaaactccg gcttccgtac cttcggtttt ggtgccggtc gtgaagacgt
     8101 ctgggaaccg gatctggatg ttaactgggg tgatgaaaaa gcctggctga ctcaccgtca
     8161 tccggaagcg ctggcgaaag caccgctggg tgcaaccgag atgggcctga tttacgttaa
     8221 cccggaaggc ccggaccaca gcggcgaacc gctttctgcg gcagcagcta tccgcgcgac
     8281 cttcggcaac atgggcatga acgacgaaga aaccgtggcg ctgattgcgg gtggtcatac
     8341 gctgggtaaa acccacggtg ccggtccgac atcaaatgta ggtcctgatc cagaagctgc
     8401 accgattgaa gaacaaggtt taggctgggc gagcacttac ggcagcggcg ttggcgcaga
     8461 tgccattacc tctggtctgg aagttgtctg gacccagacg ccgacccagt ggagcaacta
     8521 tttcttcgag aacctgttca aatatgagtg ggtacagacc cgcagtccgg ctggcgcaat
     8581 ccagttcgaa gcggtagacg caccggaaat tatcccggat ccgttcgatc cgtcgaagaa
     8641 acgtaaaccg acgatgctgg tgaccgacct gacgctgcgt tttgatcctg agttcgagaa
     8701 gatctctcgt cgtttcctca acgatccgca ggcgttcaac gaagcctttg cccgtgcctg
     8761 gttcaaactg acgcacaggg atatggggcc gaaatctcgt tacatcggac cggaagtacc
     8821 gaaagaagat ctgatctggc aagatccgct gccgcagccg atctacaacc cgaccgagca
     8881 ggacattatc gatctgaaat tcgcgattgc ggattctggt ctgtctgtta gtgagctggt
     8941 atcggtggcc tgggcatctg cttctacctt ccgtggtggc gacaaacgcg gtggtgccaa
     9001 cggtgcgcgt ctggcattaa tgccgcagcg cgactgggat gtgaacgccg cagccgttcg
     9061 tgctctgcct gttctggaga aaatccagaa agagtctggt aaagcctcgc tggcggatat
     9121 catcgtgctg gctggtgtgg ttggtgttga gaaagccgca agcgccgcag gtttgagcat
     9181 tcatgtaccg tttgcgccgg gtcgcgttga tgcgcgtcag gatcagactg acattgagat
     9241 gtttgagctg ctggagccaa ttgctgacgg tttccgtaac tatcgcgctc gtctggacgt
     9301 ttccaccacc gagtcactgc tgatcgacaa agcacagcaa ctgacgctga ccgcgccgga
     9361 aatgactgcg ctggtgggcg ggatgcgtgt actgggtgcc aacttcgatg gcagcaaaaa
     9421 cggcgtcttc actgaccgcg ttggcgtatt gagcaatgac ttcttcgtga acttgctgga
     9481 tatgcgttac gagtggaaag cgactgacga atcgaaagag ctgttcgaag gtcgtgaccg
     9541 tgaaaccggc gaagtgaaat acacagccag ccgtgcggat ctggtatttg gttctaactc
     9601 tgtcctgcgt gcggtagcgg aagtttacgc cagcagcgat gcccacgaga agtttgttaa
     9661 agacttcgtg gcggcatggg tgaaagtgat gaacctcgac cgtttcgacc tgctgtaatc
     9721 tgaccttctt cagcgactgt ctttcaggca gtcgctgaag tttctttacc ggcgtatagt
     9781 gtccacagga aaactacaca ctggatctct catgtctgcc gcaggaaaga gcaacccact
     9841 ggcaatcagt ggcctggttg tgctcacact tatctggagt tatagctgga ttttcatgaa
     9901 gcaagtcacc agctacatcg gtgccttcga ctttaccgcc ttacgctgca ttttcggcgc
     9961 tctcgtttta ttcatcgtcc ttttattacg tggtcgcgga atgcgcccga caccgtttaa
    10021 atacacctta gccattgccc tgttacaaac ctgcgggatg gttggcctgg cgcagtgggc
    10081 gctggtcagc ggcggagcgg ggaaggtggc gatcctaagc tataccatgc cgttctgggt
    10141 ggtgattttc gccgcgttgt ttctcggtga acgcctgcga cgtgggcaat atttcgcgat
    10201 actgattgcc gctttcggct tatttttggt gttgcagccg tggcaactcg atttctcttc
    10261 aatgaaaagt gccatgctgg caatcctctc cggcgtcagt tggggggcga gcgcgattgt
    10321 tgctaaacgc ctatacaccc gtcatccgcg cgtggattta ttatcgttaa catcctggca
    10381 gatgctctat gcggcgctgg tgatgagtgt ggtcgcttta ctggtgccgc aacgtgaaat
    10441 tgactggcag cccaccgtgt tctgggcgct ggcctacagt gcgattctgg cgacggcgct
    10501 ggcgtggagc ttatggttgt ttgtattgaa aaacttgcct gccagtattg ccagcttaag
    10561 cacgctggcc gttcccgttt gcggcgtact cttttcctgg tggctgctcg gtgagaatcc
    10621 gggggccgtt gaaggtagcg gtattgtgct gattgtgctg gcactggcgc tggtgagccg
    10681 taagaaaaaa gaagctgtca gtgtaaaacg catctgaatt ttttcttcat gtggggcgat
    10741 cgcttattta acaaaataac gataatgccc caccatccgc cagctaaaca gcacatcttc
    10801 ttcctgcgcg cctgcgccaa tgttatgtat caccagcggc gtaccgtcgc gggcgaagcc
    10861 atctgacacc accccaatat gtgccagtcc gttatccagt cgccaggaga caatatcgcc
    10921 cgcttgatag tcactggggt tcttgctgat ggggcgtgtt ttatcgtggc gggtaaacca
    10981 ggtttccaga ttaggcaccc gacggtgatc gatgttgctg tccgggcgct ttaactgcca
    11041 tttttgcggg tactcggcaa aattcttcgc catatcttcg tgcaccagtt tctgcaaatc
    11101 gaccttctgg ctgcgcaatg cacggatcac cacatcggaa cataccccgc gttcttgcgg
    11161 gacatcaccg ccaggatagg taagctgcat atacgccgga tcgtaaaata gcgtgctgcc
    11221 gatttgctgt ctggcaccgt ctgcgatggc aaggttggta ttggcctgga tttgtaccac
    11281 ggttggtgga acggcgggag attttaagga gtggctggta aatgccgtta gcaggctgag
    11341 cagcgccagt gaagctttca tctggttcgt ccgtgaaaat aagagtcaca gattcgacct
    11401 tccgggctaa cgattgttct gtggagaaaa tgtccagaag cggataaaaa actcctgaac
    11461 aacgtgggag tttcagatta ctggcacaga gtgccggatg cggagtgaac gccttatccg
    11521 gcctacaaaa gcacgcatat tcaatatatt gcagagattt cgtaggcttg ataaacgtag
    11581 cgcatcaggc aattttgcgt tcaaactccc ggacaagccg ggagtttgga gtaggttatt
    11641 cccactcttg caggaaacgc tgaccgtact ggtcggctac cagcagagcg gcgtaaacct
    11701 gatctggcgt cgcgccgcca ggcatgttgt gaatggtttc accttctgca catgccgctt
    11761 ctgccacaat tcgcattttc gccgggacat cttctttaat atccagttga gcgagagtta
    11821 ttggcaaacc taccgcatgg ctaagtgcag ctacggtttc gatttcctca accggtgcgt
    11881 tttccagaac cagctgcgtc agcgtaccaa atgccacttt ttcaccgtga taatagtgat
    11941 gcgcgtccgg gatagcggtc aggccgttat gcaccgcgtg agccgccgcc agcccgccgc
    12001 tttcaaaacc aacgccgctc aaataggtgt tcgcttcaat cacgtgctcc agcgccgggg
    12061 ttactacatg ctgttcggcg gcaagcatcg ctttttcgcc ttcttccagc agggtgttgt
    12121 agcacagttc agccagtgcc agcgccgcct gggtgcactt gccgcccgcc atggtggtcg
    12181 cgccgctacg agagcaagca cgcgcttcaa accaggttgc cagcgcatcg ccgatacccg
    12241 ctgctaacag acgtgcaggt gcgcccgcga cgattttggt gtcgacaatg accatattcg
    12301 ggttatttgg caacagcaga tagcggtcaa actcaccctc atcggtgtag ataacagaca
    12361 atgcgctgca cggtgcatcg gtagaggcga tagtcggtgc gatcgctacc ggaacaccca
    12421 tgaaatgtgc cagtgctttg gcagtatcga gggtttttcc gccaccgata ccgagaattg
    12481 cgccacactg cgcagtctcc gcgatgccac gcagacggtc gatctcattt tgcgaacatt
    12541 caccgccaaa cggcgcaatt tctactacca gtccagcatc tttaaagctt ttctcgacag
    12601 tggattgagc aaaacctaaa acaaatttgt cacccaccac taaccagcgt tctgccagcg
    12661 gcttcaggta ttcgcccaga cgattaatca catcagcgcc ctggatgtat ttacccggtg
    12721 attgaataat gcggtccata attgctcctt tagagatgag tagtgccaaa tgcggcattc
    12781 cagtcgtgtt cgaacttctc tatagctgac tctaccgcag gggtgttgag catttgttgc
    12841 gctacatcta agggcagggt gatggattca catcctgcca gtaaacagtc cagcgcctga
    12901 cgcggcgttt taaagctggc cgccagcacc atgctttctg gtgcgtgcat ttctaacagc
    12961 gcttgcagct cctgaaccgt acgaatgccg tccccgccct gggcatctac gcggttaaca
    13021 tacggagcaa cgtattttgc ccctgccagc gcggccagta acccttgtgc agcactgtac
    13081 actgcggtgc caagcgtagt aataccctct tttttcagca tcttaatggc ggccagacct
    13141 tcggaagtca ccgggatttt caccacaatg cccggaatgg cgtcgcgcag gcgcttcgct
    13201 tcttccacca taccctgcgc gtcacgactc atagtctgag caaacagaat gccctcatca
    13261 ccaatcgctt tttgcagacg cggcagaact tcccatatgg actccttgct ggcagcgata
    13321 atgctcgggt tagttgtcac cccggcaatg gggaatatgc gtgccagacg ttcgacttct
    13381 gcgacgttag cggtgtccag atacagttcc atgatgtttc cttttacagt tccagttcat
    13441 gttgcagcag gctggcgata gcgtcggcag aggcggcgtt aaccagcgcg ttacggaatt
    13501 cttcgtgcat gatgcggcga gcgaggcgcg agaaaatgcg catgtgctga tcgcccgcag
    13561 cgtgtttgtt cagggttaac ataatgatga attgcgcttc atcatcgccc cagcgcaccg
    13621 gcgcttgcag acgcgccacg ctgatggtgg attgctcaat gtgttctgat ttgctgtgtg
    13681 gaatggcaaa actaaagccc agaccggtag agaaaacggc ctcgcgcgcc cataagtcgg
    13741 cttccagttt acgcggatag cggcagcggc ccgccagcag caggttatcg gtcatgcctt
    13801 tgagcacttc ttctttgctg cgccagtcac tttccagcgt gatgcactcg gcggtgacca
    13861 gcggtgcgtc ctgttgggtc atgcggaatt gcgccagcag gtgttccact tccagcgaag
    13921 tacggcaggc cattgcctgg ttgagcaact tgcggcactc acggctatca agttgcgcca
    13981 tccgcgcttt cgccgccgga attgatggtg cgctcatgct gagttcatcc agccctaagc
    14041 cgaccagcaa cggcagcacg gaacctttcg ctcccagctc accgcacaga ccaatccatt
    14101 tgccctggcg atgcaccgcc tgcacggcgt aatcgagcgc ccgcaagaat gccggattca
    14161 ggctgttgta gtgacgagta accttagcgt tatcgcgatc cactgccagc agatattgcg
    14221 tcaggtcatt actaccaata ctaaagaaat caatctcttc gcagcattga tcgatgatga
    14281 acatcaccga cggtacttcc agcataatgc caagctggat cttctcatca aacggaatgt
    14341 gttcgttacg cagttgctgt ttggcttccg ccagtttttc tttcacccat aagatctctt
    14401 ccattgagga gatcatcggg atcatgattt tcaggctgcc gtgagcggag gcgcgcagga
    14461 tcgaccgtag ttgtgtggtg aacagcgagg cgtattcttc ataaatacgc acagcgcgat
    14521 aaccgaggaa cgggttagct tctgccggaa tgttcagata atcaacgggt ttgtcgccgc
    14581 caatgtccat agtgcgcaca ataatgctgc gtccgttggc agactccagc gcctgacaaa
    14641 aaatgttgta cagctcgctt tcgcccggtg cactggtgcg atccatatag agcatttcag
    14701 tgcggaacaa accaacgcct tccgcaccat tgccgaatgc ggcctgcgct tccacggagt
    14761 gagcgatgtt agcggcaatt tcaatgcgga taccgtcagc ggtacgggct tgttgggtca
    14821 gccagacacg ctgttgctca cgcagggcgt cctgtacgcg ggcttcttgc tgataataac
    14881 gagctacggc ttctcctggc tcaaccacaa tcgccccagc gttgccgtcg atataaatcg
    14941 tttgatgctg ccacggagta agggcatcaa tatctacacc aaccagtgtc ggaatgttga
    15001 acgaacgggc aagaattacg gtatgtgagg tggtgccgcc acttttcagt aacagacctt
    15061 tgaggtgatt tttatccagt tcgaggaact ggctgggagt cagttcatca gccatacaaa
    15121 tggcgggctg cgtcagtttg cccggtgccg ggaagcgttg ctcaccgtag atttgctgga
    15181 gtaactggaa gcagacgtcg cgtacgtcca gggcacgttc ttgcaggtag ctgctgctgg
    15241 aacgggcgaa ctcttcgcaa aagtgattcg cactggtgac aatcgcttcg gcgcagctta
    15301 atccggcgct gacgcctgcc agtaaatgtt cgcgcaggga agtatcgcca gccagggatc
    15361 ggtgcgcttc cagaatcgcg ctggtggcac cgtcgctatc cagcagacga aactcaatgt
    15421 ttttcagtac cagcgtcagg ccgttttcca gtgcggattg ctcggcgtca acgcctttgg
    15481 ctgcgggaag attacccagc gcattgagat ctaaagaaga gatcggcgtc agaatgccgc
    15541 ctgcactacc gctgcacacg gtgcgggcgc ggataatttg cggattcaga ttggtcagtg
    15601 aaaccggcag tggttccagt tcgtcagatt taacttccgc cagcggcgca tcgcagtggg
    15661 ggaattcatc gcgcagccat tggcttaaac gctggtgcgc ttcctgttcg tcggccccgg
    15721 aaatcagtaa ctggcagtta tcgcccgcca gcgtatcggt gccaatcagc gcaagggcgc
    15781 ttttggcgtt gcccttgcgg tcagtgcgca ggttatgcca ctcaatttgt gatgaaaaag
    15841 tattacacag cgtttcaacg tggcttgccg gacgtgcatg tacgccgtta ggtagctcac
    15901 aaataaattc cacaatcagg gccattgcct ctctcccggt acggttttct gtttacagga
    15961 taagagcgca cggcaacggc ctgccatgtg acaaatctgc caaaagctgg acaaatgtaa
    16021 tgtaaccgtc aatttgcgac gcgtctcaca agacgctgtt ttgcggcatg cttccggttt
    16081 atcgcaagtt atgaggcgga tcgcattttt gtactgatat tacaaaagtc cagtaaatgg
    16141 cctttttatc cactgtttgc tccgcctgcg attgcctatt gtttcctcca tcaaaaatat
    16201 ggacatgggc tttcgcaagc ccgggagcag cttatgaatg agttggtgca gatcctgaaa
    16261 aatacccgtc agcatttaat gacgggcgtt tcacacatga ttcccttcgt ggtatcgggc
    16321 ggtattttgc tggcggtttc cgtcatgttg tatggcaaag gcgcagtgcc ggatgccgta
    16381 gccgatccaa atctgaaaaa actgtttgat atcggcgttg cgggcttgac gctgatggtg
    16441 cctttcctcg cagcgtacat cggttattcc attgcagagc gttctgcgct ggctccgtgc
    16501 gctatcggtg cctgggttgg taacagcttt ggtgcgggct tctttggtgc actgatcgcc
    16561 gggattatcg gcggcatcgt ggtgcattac ctgaagaaaa ttccggtgca taaagttctg
    16621 cgctcggtga tgcctatctt cattattcct atcgtcggca cactgattac cgcaggcatc
    16681 atgatgtggg gcttgggcga gcctgtaggg gcgttgacca acagcctgac tcagtggctt
    16741 caggggatgc agcagggcag cattgttatg ctggcggtga tcatgggtct gatgctggcg
    16801 ttcgatatgg gcggtccggt taacaaagtg gcctatgcct tcatgctgat ttgcgttgct
    16861 cagggtgttt ataccgtggt ggccatcgct gccgtgggta tttgtatccc gccgctgggg
    16921 atggggctgg cgacgctgat tggtcgtaaa aatttctccg cagaagagcg cgaaaccggc
    16981 aaagcggcac tggtgatggg ctgtgttggc gttactgaag gggcgattcc tttcgccgct
    17041 gccgatccgc tgcgtgtcat tccttccatc atggtcggtt cagtttgtgg tgcagtaact
    17101 gcggcgctgg tcggtgcgca gtgctatgca ggctggggtg gtctgattgt gctgccggtg
    17161 gttgaaggca agctgggtta tatcgcagca gtggctgtcg gagcagtggt gacggctgtt
    17221 tgtgtgaacg tgctgaaaag tctggcgcgt aaaaatgggt cttcgactga tgaaaaagaa
    17281 gacgacctgg atttggattt tgaaattaat taattgagga acgagccatg acgaaaatta
    17341 ttgcagtaac cgcatgtcct tcaggtgttg cccataccta tatggcagca gaggcgctgg
    17401 aaagtgccgc gaaagcgaaa ggctgggaag tgaaagtaga aacccagggg tcaattggtc
    17461 tggaaaatga actgactgcg gaagatgtgg cgagcgctga tatggtgatt ctgaccaaag
    17521 atatcggcat caagtttgaa gagcgttttg ctggcaagac catcgtgcgc gtcaacatca
    17581 gcgatgcagt aaaacgtgct gacgccatta tgagcaaaat tgaagctcac ctggcgcaaa
    17641 ccgcttaagt tcttctcccg ctcgcaaggg cgggttcgct ttcccacagg agttcctcat
    17701 gacgaatcgt atctctcgcc tcaaaactgc actgtttgcc aatacccgtg aaatctcgct
    17761 ggagcgggcg ctgctttata ccgccagcca tcggcaaacc gaaggcgaac cggtgatatt
    17821 gcgccgggcg aaagcaacag cgtatatcct tgaacatgtt gaaatttcga ttcgtgatga
    17881 agaactgatt gccggtaacc gcaccgtaaa accgcgcgct gggattatgt cgccggaaat
    17941 ggacccttac tggctgctga aagagctgga tcaattcccg acgcgtccgc aggaccgctt
    18001 tgctatcagt gaagaagata aacgtatcta ccgtgaagag ttgttcccgt actgggaaaa
    18061 acgttcgatg aaagatttca tcaacgggca gatgacagat gaagtaaaag ccgcgaccag
    18121 cacgcagctt ttcagcatca accagacaga taaaggccag gggcacatta ttattgatta
    18181 cccacgcctg ctgaatcacg ggctggggga gctggtggcg cagatgcagc aacattgtca
    18241 gcaacagccg gagaatcact tttatcaggc cgcgctgtta ctgctggaag cctcgcagaa
    18301 acacattctg cgttacgctg aactggcgga aacaatggcg gcaaactgca cagatgccca
    18361 gcgtcgcgaa gaactgctga ctattgcgga gatctcccgc cataacgccg aacataagcc
    18421 gcagacgttc tggcaggcgt gccagttatt ctggtacatg aacatcattc tgcaatacga
    18481 atccaacgcc agttcgctat cgttggggcg cttcgaccag tatatgttgc cgttctatca
    18541 ggcatcttta acccagggcg aagatccggc gttcctgaaa gaactactcg aatctttatg
    18601 ggtgaaatgc aacgacatcg tgctgttgcg ctccaccagc agcgcgcgtt atttcgcagg
    18661 tttcccgacc ggctataccg cactgctcgg cgggttaacc gagaacggac gtagcgcggt
    18721 gaacgtgctt tcgttccttt gccttgacgc ttatcaaagc gtgcaattac cgcaaccgaa
    18781 cctcggcgtg cgcactaacg ccttgatcga cacgccgttc ctgatgaaaa ccgccgaaac
    18841 cattcgcctc ggcaccggta ttccgcaaat ctttaacgat gaagtggtgg taccagcgtt
    18901 cctcaaccgt ggcgtttcgc tggaagatgc gcgcgactat tccgtagtgg gctgtgtgga
    18961 attatctatt cccggcagaa cctacggctt gcatgacatc gcgatgttta atctgctgaa
    19021 agtgatggaa atctgcctgc atgaaaatga aggcaatgct gcgctgactt atgaaggttt
    19081 actggagcag atccgcgcca agatcagcca ctacatcacc ctgatggttg agggcagcaa
    19141 tatttgtgat atcggccatc gcgactgggc acctgtaccg ctgctctcgt cttttatcag
    19201 cgattgtctg gaaaaaggtc gcgatattac cgatggcggc gcgcgttata acttctccgg
    19261 cgtacagggg atcggtatcg ccaacctgag cgattctctc catgcgttga aagggatggt
    19321 ttttgagcaa cagcgtttaa gttttgacga attgctgtcg gtattaaaag ccaactttgc
    19381 aacgccagaa ggcgaaaaag tccgtgctcg cttaattaac cgcttcgaga aatacggtaa
    19441 cgatatcgac gaggtggata acatcagcgc cgaactgtta cgccactact gcaaagaagt
    19501 ggaaaaatac cagaacccgc gcggcggtta cttcacgccg ggatcgtata ccgtttctgc
    19561 tcacgtcccg ttgggatcgg tggttggcgc gacgccagac ggtcgttttg ccggggaaca
    19621 actggcagac ggcggcttgt cacctatgct gggccaggac gcacaagggc caacagcggt
    19681 actgaagtca gtcagtaagc tcgataatac gctgctgtct aacggtacgt tgctgaacgt
    19741 gaaattcact ccggcgaccc tggaaggtga agcgggatta cgcaaactgg ccgacttctt
    19801 acgggcgttt acccagctta agttgcagca tattcagttt aacgtggtga acgccgacac
    19861 gttgcgggaa gcgcaacagc gcccacaaga ttatgccggg ctggtggtgc gcgttgccgg
    19921 atacagcgcc ttctttgtcg aactgtcgaa ggagatccag gatgacatca tccgccggac
    19981 agcgcatcag ctgtaacgtt gtggaaacgc gccgcaatga tgtggcgcgc attttcaaca
    20041 ttcagcgtta ttcactgaat gacggcgagg gcattcgtac ggtggtcttt tttaaaggct
    20101 gtccgcatct ttgcccgtgg tgtgctaatc cggagtcgat ctccggcaaa atccagacgg
    20161 tacgcagaga ggcgaaatgt ctgcactgtg cgaaatgttt gcgtgatgcg gatgaatgcc
    20221 cctccggtgc gtttgaacgg attggtcgcg atatcagcct tgacgctctg gaacgggaag
    20281 tgatgaaaga tgacattttc tttcgcacgt ccggcggcgg cgtcacgctt tctggcggcg
    20341 aagtgttaat gcaggcggag tttgctaccc gttttttaca gcgactgcgg ctgtggggtg
    20401 tctcatgtgc cattgaaact gccggagacg cgccggccag caagctgtta ccactggcga
    20461 aattgtgcga tgaagtgttg ttcgatttaa aaattatgga tgcgactcag gcgcgggatg
    20521 tggtgaatat gaacctgcca cgcgtgctgg agaatctgcg tttgctggtg agtgagggcg
    20581 tcaacgtgat cccgcgttta ccgctgatcc ctggtttcac gctcagccgg gagaatatgc
    20641 agcaggcgct ggacgtgctg atcccgctga atatcaggca gatccatctg ttaccgtttc
    20701 atcagtacgg cgaaccgaaa taccgcctgc tggggaaaac atggtcgatg aaagaggtgc
    20761 ctgcgccgtc gtcagccgat gtgacaacga tgcgcgaaat ggcagaacgg gccggatttc
    20821 aggttaccgt gggaggttaa aatggcatac ctggtggcag taaccgcctg cgtaagcggc
    20881 gtggcgcata catatatggc ggcggaacgg ctggaaaagt tgtgccagtt agagaagtgg
    20941 ggcgtcagca ttgaaactca gggcgcgttg ggaacggaga atcgtttagc ggacgaggat
    21001 atccgtcggg cggatgttgc tctgttgatt acggatatcg agcttgccgg tgccgagcga
    21061 tttgaacatt gccgctatgt gcaatgcagc atctacgcat tcctgcgtga gccgcagcgg
    21121 gtaatgagcg cggtgcgcaa agtgctttct gcaccgcagc aaacccatct tattctggag
    21181 tagtcggttt ttctgtcagt tggctgtggt actgccggcg atattccgac ggcgagcgtt
    21241 cggtgttttt acgaaacaga cggcagaagt agttgctgtc gacaaagccg caggcgtgcg
    21301 ccacttcttt taccttcagg tcatagcctt tcaataacgt cttagcgtgc tccagtcgcg
    21361 tgtgattcag gtattcgtta aaaccaatgg cccccgtttt ttgaaacagg tgcgagagat
    21421 agtttggtga aatatagaat gcctgtgcga cagattcgcg ggtaagcgcg gaggcatagc
    21481 gttcgtcgat ataatcgcga atagcttcaa atagtgcctg gctgcgtgag gcggtctgga
    21541 tttggctgcc gagcaaatcg cggcagtggc taagcaggct ggctacgata agccttgccg
    21601 tttgctgctc ctgcggctgc atttgcattt cattgagcgt ttgtagcaga aaagaaccta
    21661 tacgtgggcc gcgtcgggcg acgtgttgct tcgccagatt ttgatattgt ttgccatccc
    21721 attgcacgac gctgaagccg agttgctgtt tgccaaacag cacgctaaag gtggtagcgg
    21781 gtgttttcca ttgtgggaaa ttccagccac cagcggcaac gtacagcaca tcgccgggaa
    21841 ctaacgttgc tccagcgccg gtatcaacaa actcaccttc cagcacaatt tccagccgtg
    21901 gaaaatcgac ctgatacgca agatcgggca ccgggccgtt actgctggca aagtaaatct
    21961 ggcgcaggga cagcgggccg ttgatcaggc gggagagcag atagctgacg tcgtgataca
    22021 tgttattccc ttaggtatgt agacgggaaa acagtaactg gcattattaa aaaaggccac
    22081 ctgaaaagtg tggcctgagg ggagttcgat gtaaacattg cggcagtatc gcatccggca
    22141 aatagcgcca gacgcgatac aaaggtcatt tactgattac ctacctggtc gccatacggc
    22201 agagtgtcgt aatcgatcag cgcgtttttc ttgtacgggt taccaatcca gcgggtagtt
    22261 tctttgaact gcggattcac cactgaacgg gttggatcgt aaccgtcgta agataagccc
    22321 gccaaatctg accaggtgtg gatcagttcc gccaggctgt atttacgatc gacatcctgt
    22381 gagaaatcac ggggatgagt cgcttgccat ttttccgacg tccacagcag gaacggaatg
    22441 gtgtacatgt ggcgcgtcgg gttgtcttcg ttacgcccct gagttttgtg cggcggcgtg
    22501 tcgtaaacct cttcaccgtg gtcagagaag taaaccagga atccgttcgg gtctgccgct
    22561 ttaaagtctt taatcaggct ggcaaccaca tgatcgttat acaggttagc gttgtcataa
    22621 tcgttatatg attccagctc ttctgcgctt aatcctggcg gaacatgatc ggtattgcca
    22681 tcaaacttgc cctgattttc cgggtagcgg tatttgtatt tgatatgcgt acccagcaga
    22741 tgaacgatga tcagtttctt cggcgcaggg tcattcagca catcctggaa cggcttcagc
    22801 acgttggtgt cgtattcacg cgcactctgc gtacgttgct ggttcatgta gtactgcttg
    22861 tcggtctggc gcgaaaatac cgtcagcatg gtattgcggg cggtcatcgt ctgctggttg
    22921 gtgatccaga aggttttata acccgcctgt ttcatcatgt tcatcagcga cggctgcgtc
    22981 agatacagat ccgggttctt ttcattggca aaggtcagcg cctgttgcag gatttcaatg
    23041 gtgtacggac gagacgtaac tacgttatta aacacggtca gattcggatc ggttttatgc
    23101 agcgcatcca gctccggcgt ggtttcacgc ggataaccgt acagactcat gcgtccgcgc
    23161 tgggtcgact cgccaatcac cagcactaaa gtgcgcggtt cgttacccga ttcatctttg
    23221 aaattagcca gtggcggcaa ggcattattt tcattcagta actttgttag cgagtttagt
    23281 tgctgacgat actgataata gccggtcagg aattgccacg gtgcggcagg ctccatacgc
    23341 gaggccaggt tatccaacgt tttctcaaac ggcttgtttt tgataaacgt attcatggcg
    23401 atcggatgca gaatcaagcc ataaagcagg gcaaaagaga caacataacg ccacggcttt
    23461 ggaatataga ccgggcgcag gcgtgtccac agcagaactg ccaccgccgt ataggccagc
    23521 gcgataagca caattttcag gctgaaatac tggcttaaat actcgctggc ttcgttggtg
    23581 ttggtttcga acatcacaaa cagaacgctc tgcgagaact cctgcccgta gatgacgtag
    23641 tagcatagcg ccgccagaga ggccgcccat agcaccacgc cgattactgc ggcaataatt
    23701 ttaatccgct tcggaaagag gaataccggg atcaaccaca gcgagctgaa taacagcgag
    23761 tcgcgaatgc cgttggtgcc actataacca ctgatgtaaa taatggcctg tagcagagta
    23821 gagaaaaacc aaaagtagag cagtgcccaa cccagggctt tccagctaaa aaggggttta
    23881 gcctggactt ctgtggaatg catagtaaga acctgtcttg aaaaaaatat cgccgaatgt
    23941 aacgacaatt ccttaaggat atctgaaggt atattcagaa tttgaataaa atgcagacag
    24001 aaatatattg aaaacgaggg tgttagaaca gaagtatttc agaaaaccct cgcgcaaaag
    24061 cacgagggtt tgcagaagag gaagattagc cggtattacg catacctgcc gcaatcccgg
    24121 caatagtgac cattaacgct tgttcgacgc gaggatccgg ttcctggcct tctttttctg
    24181 cctggcggga gcggtgcagt aactcggcct gcaatacgtt cagcgggtcg gtgtaaatat
    24241 tccgtagctg aatagactct gcaatccacg gcagatcggc cattagatgg gaatcgttgg
    24301 caatcgccag caccactttg atgtcttctt cttgcaggtt gcgtaactct ttacctaacg
    24361 gccacagtgc tttgtctacc aggcgttggt catagtattc cgccagccac aggtctgctt
    24421 tggcgaagac catctccagc atgccgagac gcgtcgagaa gaatggccaa tcgcggcaca
    24481 tggcttccag ctcgctctgt ttgccatctt cgaccacttt ttgcagcgcc gtacctgcac
    24541 ccagccaggc ggggagcatc aggcggtttt gcgtccaggc aaaaatccac ggtatggcgc
    24601 gcagcgactc gacgccgccg gttgggcgac gtttcgccgg acgtgaaccc aacggcagtt
    24661 tgcccaattc ttgttccggc gtagcggagc ggaagtaagg cacaaaatct ttgttttcac
    24721 gtacgtagcc acggtagaga tcgcaggaga tgactgacag ttcatccata atgcgacgcc
    24781 agctctcttt cggctcaggt ggtggcagca ggttggcttc cagaatcgcc ccggtgtaca
    24841 gcgacaggct gctgacggtg atttctggca gaccatattt aaagcggatc atctcgccct
    24901 gttcagtcac gcgcaggcca cctttcaggc ttcctggcgg ttgtgacagc agcgccgcat
    24961 gagcaggtgc gccgccgcga ccaatggaac cgccgcgacc gtggaacaac gtcagctcaa
    25021 tacccgcttt ttcgcaggtt ttgattaatg catcctgtgc ctgatattgc gcccaggaag
    25081 ctgccatcac tcccgcatct tttgctgagt cggaatagcc aatcatcacc atctgtttgc
    25141 cctgaatcag gccacgatac cagtcaatat tgagcagctg ggtcatgaca tcgttggcgt
    25201 tgttcagatc atcgagggtt tcaaacagcg gagcaaccgg catcgcaaac ccgatacccg
    25261 cttctttcag cagcaggtgg acagccagta cgtcggacgg cgttttcgcc atcgagatca
    25321 cgtaggcggc aatggagcct tgcggcgctt cggcaatcac ctggcaggta tcgagcactt
    25381 cgcgcgtttc ggcgcttggt tgccagttgc gcggcagaag cggacgtttg gagttcagtt
    25441 cgcggatcag gaacgcctgt ttgtcggcct ctgaccagct ttcgtagtcg ccgataccga
    25501 ggtagcgggt cagctcgccc agcgcttcgg tatgacgcgt gctctcctga cggatatcaa
    25561 tacggaccag cggtacgccg aaacatttca cgcggcgcag ggtgtcgagc agatcgccgt
    25621 tggcgataat acccatgcca cacgcctgaa gtgactggta gcaagcgtag agcggttccc
    25681 acagttcttc gttttgtgtc agcaggcctt ctggttttgg cagttcttcg cctttcaggc
    25741 gcgcttccag ccatgcctgt gtcgccatca ggcgagaacg caggtttttc atcagatagc
    25801 gatacggttc tgcggcacct tcttcgccaa ccagcgccag cagttcaggg gtcgcttcaa
    25861 ccatcgacag ttcagaaacc agcacctgaa tatctttcag gaacaaatcg gtggctttcc
    25921 agcggctgag tagcaggacg tggcgggtga tatcggcagt gacgttcggg ttgccgtcgc
    25981 ggtcgccgcc catccacgaa gtaaaacgga ccggaacaaa ttcgacgggc agtttgtagc
    26041 cgaggttctc ttccagttgt tcgttcagtt cgcgcaggta atttggtacg ccttgccaca
    26101 ggctgttttc cactacggca aagccccatt tggcttcatc taccgggctt ggacgcagct
    26161 tacggatttc atcggtatgc catgactggg cgattaactg gcgcaggcgg cgcatcagct
    26221 ggttgtgttc gtagtcagcg atatctttgt tatcgagctg ttttaaacag gcgttcactt
    26281 ccaccatttt gtggatcagt gtacgacggg taatttcggt tgggtgagcc gtgaggacca
    26341 gttccagcga cagcgattcc actgcttttt tgatggtgtc ttcgctcagt tccggctggt
    26401 ttttcagttt acgcagggtg cgggcgatca cttccgggtt gctggcagct tcgcctttcg
    26461 gcgaaatgct gtggtattgc tcggcggtgt tggccaggtt caggaactga ctaaacgcac
    26521 gcgcaacggg cagcagctcg tcgttcgaca aattttgtaa ggtggtgagc aactcctggc
    26581 ggttagcatc attgccagcg cgtgaagatt tcgacaactt acggatagtt tctacgcgtt
    26641 caagaatgtg ttctcccaac gcatccttga tggtttctcc cagcactttg ccgagcatac
    26701 tgacattact acgcaatgcg gaatattgtt cgttcatatt accccagaca ccccatctta
    26761 tcgtttgata gccctgtatc cttcacgtcg cattggcgcg aatatgctcg ggctttgctt
    26821 ttcgtcgtct tttataaagc cacgtaaaag cggtgacgtc aaatgctgcg aaatcgcttc
    26881 agcaaacgaa taaatagcag gaatttacgc cattaaattc acgacgcttt aaataagcgt
    26941 aacttatgga aatgttaaaa aatcgccata cataacacca aaggtgtagg tcggataaga
    27001 tgcgcaagca tcgcatccga cattattgcg gcacaggagt ttggcaacgg tgccggatgc
    27061 ggcgcgagcg ccttatccgg cctacgtttt aatgccagca aaaatggtga attacctggg
    27121 ttatcagttc gcgggtgggc ttgataaacc gtgtttccag atattcatca ggttgatgag
    27181 cctgattaat tgagccaggc cccaacacca gcgtcgggca taacgtttga ataaacggcg
    27241 cttcggtaca gtagttcacc acttcggtct ttgctccgag caatttctca actacttcaa
    27301 ccagttgatg attcggtggg cattcatagc cagggatcgg cggatgcagc tcgtcgaccg
    27361 tcagacgacc cggccagcgt tcgctcaccg gagccaatgc atcgttgagc aaaccattaa
    27421 gttcattgag tgtcatgcca ggcagcggac gaatatccat atgcaactca cagcaagcgc
    27481 aaatacggtt agaagcgtcg ccaccgtgaa tatgcccgag gttgagcgta gggtatggca
    27541 cggtaaacgc ttcgtagtga taacgttctt tcaggttatc gcgcaattgc aaaatatgcc
    27601 cgatggcgtc gtgcattagt tcgatagcgt taactccgcg tgctggatcg ctggagtgcc
    27661 ccgactggcc ctgaatacgg atggcgttag agatatgacc tttatgtgcg cgtaccggtt
    27721 gtagtgacgt cggttcgcca atgatggcgc aatccgggcg cagggcggta gtttcggcaa
    27781 aataacgcgc tccggccata ctggtttctt catcagcagt cgccagaatg tagagcggtt
    27841 ttttcagttt cgtgacgtcg acatcgcgta gcgcatcaag gataaacgca aaaaagcctt
    27901 tcatgtcggc ggtgcctaag ccgtaaagct tgccgtcatg ctccgtcagt gtaaacggat
    27961 cgcgcgtcca gcgaccgtca tcaaatggca ccgtatcggt atgccccgcc agcaacaagc
    28021 cgccagcccc ctgtccgata cttgccagca tattgaattt gttgcgagtt cctggcacag
    28081 gctgcacttc cacattgaag cccaaatctt taaaccagtc cgccagcaga gtgattaaat
    28141 ctgcattgct ttgatcgagt gcctcttccg tggcgcttat tgaaggtgtg gcaatcagag
    28201 cacggtaaat ctcgataaat ggcggtaatt tgtttttcat tgttgacaca cctctggtca
    28261 tgatagtatc aatattcatg cagtatttat gaataaaaat acactaacgt tgagcgtaat
    28321 aaaacccacc agccgtaagg tgaatgtttt acgtttaacc tggcaaccag acataagaag
    28381 gtgaatagcc ccgatgttga atacgctgat tgtgggtgcc agcggctacg ctggcgcaga
    28441 gctagtgacc tatgtaaatc gccatccgca tatgaacata accgctttga ctgtttcagc
    28501 gcaaagcaat gatgcgggaa agttaatctc cgatttgcat ccgcagctaa aaggcatcgt
    28561 tgatctgccg ttgcagccga tgtcggatat cagcgagttt agcccagggg tggacgtagt
    28621 gtttctcgcc accgcccatg aagttagcca cgatttagcg ccgcagtttc ttgaagcggg
    28681 ctgcgtggtg ttcgaccttt ccggcgcgtt tcgtgttaac gacgccacct tctatgaaaa
    28741 atattacggc tttacccatc aatacccgga actgctggaa caggcagcct acggtctggc
    28801 ggagtggtgc ggtaataaat taaaagaagc gaatttgatt gcggtgccag gctgttatcc
    28861 gacggcggca cagctggcgc tgaaaccgtt gattgatgcc gatcttcttg acctcaatca
    28921 gtggccggtg atcaacgcca ccagcggcgt gagcggtgca ggacgtaagg cagcgatttc
    28981 aaatagtttt tgtgaagtta gcctgcaacc gtatggcgtc tttactcatc gccatcaacc
    29041 agagatcgcc acacacctcg gtgctgacgt tatcttcact ccgcatctgg gcaatttccc
    29101 gcgcggcatt ctcgaaacca ttacctgccg cctgaaatcg ggtgtgaccc aggcgcaagt
    29161 cgcgcaagtg ttacagcagg cgtatgccca taaaccgctg gtgcggctgt atgacaaagg
    29221 cgttccggcg ctgaaaaatg tcgttgggct gccattttgc gatatcgggt ttgccgttca
    29281 gggcgagcat ctgattattg tggcgaccga agacaactta ctgaaaggcg cggcggcaca
    29341 agcggtacag tgcgccaata ttcgtttcgg ctatgcggaa acgcagtctc ttatttaagg
    29401 gtgcaatgat gaatccatta attatcaaac tgggcggcgt actgctggat agtgaagagg
    29461 cgctggaacg tctgtttagc gcactggtga attatcgtga gtcacatcag cgtccgctgg
    29521 tgattgtgca cggcggcggt tgcgtggtgg atgagctgat gaaagggctg aatctgccgg
    29581 tgaaaaagaa aaacggcctg cgggtgacgc ctgctgatca gatagacatt atcaccggag
    29641 cactggcggg aacggcaaat aaaaccctgt tggcatgggc gaagaaacat cagattgcgg
    29701 ccgtaggttt gtttctcggt gacggcgaca gcgtcaaagt gacccagctt gatgaagagt
    29761 taggtcatgt tggactggcg cagccaggtt cgcctaagct tatcaactcc ttgctggaga
    29821 acggttatct gccggtggtc agctccattg gcgtaacaga cgaagggcaa ctgatgaacg
    29881 tcaatgccga ccaggcggca acggcgctgg cggcaacgct gggcgcggat ctgattttgc
    29941 tctccgacgt cagcggcatt ctcgacggca aagggcaacg cattgccgaa atgaccgccg
    30001 cgaaagcaga acaactgatt gagcagggca ttattactga cggcatgata gtgaaagtga
    30061 acgcggcgct ggatgcggcc cgcacgctgg gccgtccggt agatatcgcc tcctggcgtc
    30121 atgcggagca gcttccggca ctgtttaacg gtatgccgat gggtacgcgg attttagctt
    30181 aagttttgtt ggccggaggc gcagctttcc ggcattgaat ttcaaaataa ggaaacagag
    30241 ttatggcact ttggggcggg cgttttaccc aggcagcaga tcaacggttc aaacaattca
    30301 acgactcact gcgctttgat taccgtctgg cggagcagga tattgttggc tctgtggcct
    30361 ggtccaaagc cctggtcacg gtaggcgtgt taaccgcaga agagcaggcg caactggaag
    30421 aggcgctgaa cgtgctgctg gaagatgttc gcgccaggcc acaacaaatc cttgaaagcg
    30481 acgccgaaga tatccatagc tgggtggaag gcaaactgat cgacaaagtg ggccagttag
    30541 gcaaaaagct gcataccggg cgtagtcgta acgatcaggt agcgactgac ctgaaactgt
    30601 ggtgcaaaga taccgttagc gagttactga cggctaaccg acagctgcaa tcggcgctgg
    30661 tggaaaccgc acaaaacaat caggacgcgg tcatgccagg ttacactcac ctgcaacgcg
    30721 cccagccggt gacgttcgcg cactggtgcc tggcctatgt tgagatgctg gcgcgtgatg
    30781 aaagccgttt gcaggatgcg cttaagcgtc tggatgtcag cccattaggc tgtggcgcgc
    30841 tggcgggaac ggcctatgaa atcgaccgtg aacagttagc aggctggctg ggctttgctt
    30901 cggcgacccg taacagtctg gacagcgttt ctgaccgtga ccatgtgttg gaactgctgt
    30961 cggctgccgc tatcggcatg gtgcatctgt cgcgttttgc tgaagatctg attttcttta
    31021 acaccggcga agcggggttt gtggagcttt ctgaccgcgt gacttccggt tcatcattaa
    31081 tgccgcagaa gaaaaacccg gatgcgctgg agctgattcg cggtaaatgc ggtcgggtgc
    31141 agggggcgtt aaccggcatg atgatgacgc tgaaaggttt gccgctggct tacaacaaag
    31201 atatgcagga agacaaagaa ggtctgttcg acgcgctcga tacctggctg gactgcctgc
    31261 atatggcggc gctggtgctg gacggcattc aggtgaaacg tccacgttgc caagaagcgg
    31321 ctcagcaggg ttacgccaac gccaccgaac tggcggatta tctggtggcg aaaggcgtac
    31381 cgttccgcga ggcgcaccat attgttggtg aagcggtggt ggaagccatt cgtcagggca
    31441 aaccgctgga agatctgcct ctcagtgagt tgcagaaatt cagccacgtg attggcgaag
    31501 atgtctatcc gattctgtcg ctacaatcgt gcctcgacaa gcgtgcggca aaaggcggcg
    31561 tctcaccgca gcaggtggcg caggcgattg cttttgcgca ggctcggtta gagtaatttg
    31621 tttatattac tcccctgaaa atggggagct taccacgcct acaaagtgcg acacttcgta
    31681 ggcggataag gcgtttacgc cacatcaggc agaaatttgt cagcattctg gtgaccttaa
    31741 atataacgcc caaccagaac cattatttgt cacattcctt tttatcattg tgtcttcttt
    31801 accttcacac tccccatgaa aatcatattt tcaaatacat caaccaatac atattttaat
    31861 taattgaata taattatttt ctattgctgt gcccattttt aatgattaat ggagtttgtt
    31921 atgtgattat ttaacgtgtt gttttaacga taatggttaa ttattcaggt ggtttttgag
    31981 ggtatttttt ctcctcggtg gaaatttttg tttttaatgt gatcacggtt gaataatctc
    32041 tttttctctc ttgtatggta gtaggcgtaa aattaagttt gaaacgtcta tttacaacac
    32101 aggagattgc aataatgaaa attattgctg cagatgtttt tgttacttgc ccagggcgta
    32161 atttcgttac gcttaaaata accactgaaa gcggactttg tggattagga gatgccacgt
    32221 taaatggtcg cgaattgtcg gtcgcttctt atctgaaaga tcatctttgt ccacaactaa
    32281 ttggccgcga tgccagccgt atcgaagata tctggcaatt cttttataaa ggtgcttact
    32341 ggcgacgtgg ccccgtcacg atgtcagcca tttctgccat tgatatggcg ttgtgggata
    32401 tcaaagctaa agcggcaaat atgccattgt atcaactgct tggtggtgct tctcgcgaag
    32461 gcgtgatggt ttattgccac accaccgggc ggacgattga tgaagtcctt gaagactatg
    32521 caaagcacca gcagatgggc tttaaggcga ttcgtgtgca gtgcggtgta ccgggtatgc
    32581 aaactactta tggcctggcg aagggcaaag gcctggcgta tgaaccggca accaaaggcc
    32641 tgtggccaga agagcagttg tggtcttcag aaaagtatct cgattttacg ccgaagttat
    32701 ttgaagcggt gcgtaataag ttcggtttta acgaacatct gttgcatgac atgcaccatc
    32761 gcctgacccc catcgaagcg gcgcgctttg ggaagagcat tgaggattac cgcctgttct
    32821 ggatggaaga tccgacgcct gcagagaacc aggaatgttt tcgtctgatt cgccagcata
    32881 ccgtgacgcc gattgcggtc ggtgaagtgt ttaacagtat ctgggactgc aagcagttaa
    32941 tcgaagagca gctgattgac tatattcgca cgacgatcac ccatgcaggc ggcattaccg
    33001 ggatgcggcg gattgccgat tttgcatcgc tttatcaggt acgtaccggt tcgcatggcc
    33061 cttcagattt atcgcctgtt tgtcatgcgg cggcgctgca ttttgaccta tgggtaccaa
    33121 attttggcgt tcaggagtac atgggctatt ccgaacaaat gcttgaagtt ttcccgcaca
    33181 gctggcgctt cgaggaaggt tatatgcacc caggcgatga accggggctg ggtatttctt
    33241 tcgatgagaa actggcggcg aaatatccgt atgacccggc gtatctccct gtcgcacgac
    33301 tggaagatgg cacattgtgg aactggtaat aacggttcgc ttaattattt tctgaatagt
    33361 catcaaatcg attaattccg ctcaaggagc taaaaatgga cgttgatgtg tcaaccagtg
    33421 ttgcgggtaa taaaccgcaa cggattcgtc gtattcagac agtgaccctg gttttattat
    33481 ttatggcggg gatcgttaat tttctcgacc gttcgtcatt gagcgtggca ggtgaagcga
    33541 ttcgtggcga gttaggatta tcggcgacag agtttggtgt tttgctttct gcattttctc
    33601 tgtcttatgg tttttcacaa ctaccttccg gtattttgct agaccgcttt ggcccacgaa
    33661 ttgttttagg cgcaggctta atattctggt cattaatgca ggcattaaca ggaatggtta
    33721 attcttttag ccactttatt ttaatgcgta tcggtctggg tattggcgaa gcgccattta
    33781 tgcctgcggg agtaaagtcg atcaccgact ggtatgcaca aaaagaacgc ggcacggcgc
    33841 tggggatttt taactcatct accgttatcg gtcaggctat cgcgcctcct gctcttgtat
    33901 tgatgcagct ggcatggggc tggcggacga tgttcgttat catcggcgtg gcagggattc
    33961 tggttgggat ctgttggtac gcgtggtatc gcaaccgggc gcagtttgtc ctgactgacg
    34021 aagaacggac gtatctctcc gcgccggtta aaccgcgtcc acagctgcaa tttagcgagt
    34081 ggctggcgct gtttaagcat cggacaacct gggggatgat tttaggtttc tctggcgtca
    34141 attataccgg ttggctctac atcgcgtggt tacccggtta tttgcaagcc gagcaaggtt
    34201 tcagtctggc gaaaaccggt tgggtggcgg cgattccttt cctcgcggcg gcagtcggga
    34261 tgtgggttaa cggtattgtt gtcgatcgac tggcgaaaaa aggctacgac ctggcgaaga
    34321 cgcgtaaaac ggctattgtc tgcggtttga tgatgtcggc attaggcacg ttgctggtcg
    34381 tgcaatcttc ctcgccagcc caggcggtag cgtttatctc aatggcgcta ttctgcgtgc
    34441 atttcgctgg aacgtctgca tgggggctgg tgcaggtgat ggtgtcagaa acaaaagtgg
    34501 cttccatcgc cgggattcaa aactttggca gttttgtttt tgcttccttt gctccgatcg
    34561 taaccggttg ggtagtggat accacacact cgtttaatct ggcgttggtt atcgcggcct
    34621 gtgtgacgtt caccggggcg ctgtgttact tctttattgt caaagatcgc attgagtaat
    34681 cgttactatc aggctctctt gctgtgggcc tgtagaataa aaaaaagcgg atcctggaga
    34741 tccgcaaaag ttcacgttgg ctttagttat tcgagttgag aaactctcga aacgggcagt
    34801 gacttcaagg gttaaaagag gtgccgctcc gttcctgtga gcaattatca gtcagaatgc
    34861 ttgataggga taatcgttca ttgctattct tcctatcgcc atgaactatc gtggcgatgg
    34921 aggatggata atgaatattc gtgatcttga gtacctggtg gcattggctg aacaccgcca
    34981 ttttcggcgt gcggcagact cctgccacgt tagccagccg acgcttagcg ggcaaattcg
    35041 taagctggaa gatgagctgg gcgtgatgtt gctggagcgg accagccgta aagtgttgtt
    35101 cacccaggcg ggaatgctgc tggtggatca ggcgcgtacc gtgctgcgtg aggtgaaagt
    35161 ccttaaagag atggcaagcc agcagggtga gaccatgtcc gggccgctgc atattggttt
    35221 gattcccacg gtcgggccgt acctgctacc gcatattatc ccgatgctgc accagacctt
    35281 tccaaagctg gaaatgtatc tgcatgaagc acagacccac cagttactgg cgcaactgga
    35341 cagcggcaaa ctcgattgcg tgatcctcgc gctggtgaaa gagagcgaag cattcattga
    35401 agtgccgttg tttgatgagc caatgttgct ggctatctat gaagatcacc cgtgggcgaa
    35461 ccgcgaatgc gtaccgatgg ccgatctggc aggggaaaaa ctgctgatgc tggaagatgg
    35521 tcactgtttg cgcgatcagg caatgggttt ctgttttgaa gccggggcgg atgaagatac
    35581 acacttccgc gcgaccagcc tggagacgct gcgcaacatg gtggcggcag gtagcgggat
    35641 cactttactg cctgcgctgg ctgtgccgcc ggagcgcaaa cgcgatgggg ttgtttatct
    35701 gccgtgcatt aagccggaac cacgccgcac tattggcctg gtttatcgtc ctggctcacc
    35761 gctgcgcagc cgctatgagc agctggcaga ggccatccgc gcacgaatgg atggccattt
    35821 cgataaagtg ttaaaacagg cggtttaaac cgtttaacgc agctacccga taggcttccg
    35881 ccatggtcgg gtagttaaag gtggtgttga cgaagtactc aatagtgttg ccgccacctt
    35941 tctgttccat aatcgcctga ccgatatgaa taatttcggc agcgcgctcg ccaaagcagt
    36001 gaatacccag aatctctttt gtttcccgat ggaacaaaat tttcagcgtg cccacgttca
    36061 tgccgacgat ttgtgcgcgt gccagatgtt taaactgggc gcggcccact tcatatggca
    36121 ctttcatcgc ggtcagctgc tgttcggttt tgcccacaga gctgatttcc gggatggtgt
    36181 aaatgccggt agggatatct tcaatcagat gcgcggtggc ttcgcctttt accagcgcct
    36241 gcgcggcaat gcgcccctgg tcataggccg ccgacgccag gctcggataa ccaatcacgt
    36301 cgcccaccgc gtaaacgtgc ggctgtgcgg tctgatacat gctgttgacc ttcagctgtc
    36361 cgcgactgtc agtttccagc ccaatgttct gtaacgccag cgaatcagta ttaccggtgc
    36421 gaccgttggc atagagcagg cagtcagctt tcagtttttt acccgacttc agatgcatga
    36481 tcacaccgtc gtcacagcct tcgatcttct cgtactcttc gttgtggcga atcactacgc
    36541 cactgttcca gaagtgatag gagagagaat ctgacatctc ttgatcgaga aatgccagca
    36601 gccgatcgcg ggtgttgatc agatccactt ttacatccat accgcggaag atcgacgcat
    36661 attcacagcc gatcactcca gcaccataga taagtacatg gcgcggttcg tggtgcatgc
    36721 taagaattga gtcgctgtcg taaatgcgtg gatgggtgaa atcaacatct gttggatgat
    36781 atggacgaga gccgcaggca ataacaaatt tttcagcggt tagtgtttca acgctgccgt
    36841 ccgggcaatc cagcgccaac gtatgctcgt caacaaagcg agcgtttccc tgcaatattt
    36901 cacagtgatt acgttcgtaa aatccctgac gcatgcgcgt ttgttgatta atcacgttat
    36961 cggcatggtt aaggatatcg gcaaaagaag agcggagcag tcgggaatgg tcgctgtaaa
    37021 gtgggttttg attgaattct ataatgcggc tgacggcgtg acggagagct ttcgacggga
    37081 tggtgcccca gtgggtgcaa ccgccgccaa cattttgata acgctcgata actgcgacgc
    37141 gcgcaccttg cttaaccagg cccattgcag cgccttcgcc gccggggccg gaacctatta
    37201 ctatggcatc gtaatcgtag gaatgtggca tggtagggct tacctgttct tatacataaa
    37261 agcaacagaa tggtaacatt ttatcgcggg taagccaatt gatccccgtc atttatctgg
    37321 ctatatcctg agcggccttt gctttgtctg tttcttactt ttgccctgac gttttattgg
    37381 atttttatcg acgatactct ccgtttaagc ggcaggtttc cgctgtacgt aaaagaaccg
    37441 gccagtaaat atgttttatt gcgttaccgt tcattcacaa tactggagca atccagtatg
    37501 ttcattctct ggtatagtgc cagcagtact tttggcaagg attcagacat cgtgatgggc
    37561 ataagagcgc aacaaaaaga aaaaacccgc cgttcgctgg tggaagccgc atttagccaa
    37621 ttaagtgctg aacgcagctt cgccagcctg agtttgcgtg aagtggcgcg tgaagcgggc
    37681 attgctccca cctcttttta tcggcatttc cgcgacgtag acgaactggg tctgaccatg
    37741 gttgatgaga gcggtttaat gctacgccaa ctcatgcgcc aggcgcgtca gcgtatcgcc
    37801 aaaggcggga gtgtgatccg cacctcggtc tccacattta tggagttcat cggtaataat
    37861 cctaacgcct tccggttatt attgcgggaa cgctccggca cctccgctgc gtttcgtgcc
    37921 gccgttgcgc gtgaaattca gcacttcatt gcggaacttg cggactatct ggaactcgaa
    37981 aaccatatgc cgcgtgcgtt tactgaagcg caagccgaag caatggtgac aattgtcttc
    38041 agtgcgggtg ccgaggcgtt ggacgtcggc gtcgaacaac gtcggcaatt agaagagcga
    38101 ctggtactgc aactgcgaat gatttcgaaa ggggcttatt actggtatcg ccgtgaacaa
    38161 gagaaaaccg caattattcc gggaaatgtg aaggacgagt aatgaaacaa gcaaatcaag
    38221 atagaggtac gctgctgctg gcgttagttg ctggcttatc gattaatggt actttcgcag
    38281 cgctgtttag ctccattgtg ccattttctg tattcccgat tatttccctg gtgctgacgg
    38341 tttactgcct gcatcaacgt tatcttaatc gcactatgcc ggtaggcttg ccgggtctgg
    38401 cagctgcctg ttttattctc ggcgtactgc tgtacagcac ggtagttcgt gcggaatatc
    38461 cggatatcgg ctctaacttc ttcccggcag tactctccgt cattatggtg ttctggattg
    38521 gcgcgaagat gcgtaaccgt aagcaggaag ttgctgagta atcggtatta tgccggatgc
    38581 tggcgcatcc ggcatgggtt ttacttcgcg gtcagtaata cgccgcactc catatggtgc
    38641 gtgtagggga actgatcaaa cagagccaga cgttcgacct tgtgcgtctg gcttaatgtt
    38701 tccagattct tgcataacgt ttccgggtta caggagatgt acaaaatacg cggatacgcc
    38761 tgcaccattt tctcggtttc actgtccaga ccgctgcgcg gtgggtcgac aaaaatggtt
    38821 tcgcactgat aactctttaa gtcgatccct tgcaggcggt taaactcgcg cacaccattc
    38881 atcgcctgag taaattcttc tgccgccata cgaataattt gtacgttatc aatatggtta
    38941 gctgcgatgt tgtattgcgc agcagcaacc gacggcttag cgatttcggt ggctaatacc
    39001 cgatcaaaat tgcgcgccag cgctaatgaa aagttaccgt tgccgcagta cagctccagt
    39061 aaatcgcctt ttgagccttt ggttacgtcc agcgcccatt ccagcatctg aatattcatc
    39121 gccgcgttcg gctgggtaaa actgttttct acctgacggt agatcatctc tttccctgcg
    39181 accggcagac gttcatcgat gtaatcttga tccagctcga ttttggtttt cgttgcccga
    39241 ccaatcagat gcacattcag attctgcgcg cgcagtgcat cgcgcagggc ctccgcttcc
    39301 tgacgccact catcatccag cttcttatgg tatagcaggg aaaccaccgc ctgattactc
    39361 agcgtggtga ggtaatcaat ctggaacaac ttgtggcgca gaacgggatt attacgcaca
    39421 cccgcaatca tcgccgtcat caactggttg atgagttcac tggcagcggg gaagctatcc
    39481 acgcggatgc ggcttttggt ttgttgatcg aaaatgatgt gatacaggtc atcgccatcg
    39541 tgccagatgc ggaactccgc acgcatccgg taatgactga ccggcgagcg aaacacttcc
    39601 ggaaccaggt cagaaaacgg tgccatcata ctttgcaaac gtaccacttt ttcggctaac
    39661 tgcgcttcat actgttctgt tggaaggtgt tcgggggtca tgatgtatcc tgaaaaatta
    39721 aagtacgcgg cgattgtagg gattgctcat cagatgtcca gatcttgatg aattcctatt
    39781 tgtgagctac gtctggacag taacttgtta caacctgtag catccacttg ccggtcctgt
    39841 gagttaatag ggaatccagt gcgaatctgg agctgacgcg cagcggtaag gaaaggtgcg
    39901 atgattgcgt tatgcggaca ctgccatccg gtgggaagtc atcatctctt agtatcttag
    39961 atacccctcc aagcccgaag acctgccggc caacgtcgca tctggttctc atcatcgcgt
    40021 aatattgatg aaacctgcgg catccttctt ctattgtgga tgctttacaa tgattaaaaa
    40081 agcttcgctg ctgacggcgt gttctgtcac agccttttcc gcttgggcac aggataccag
    40141 cccggatact ctcgtcgtta ctgctaaccg ttttgaacag ccgcgcagca ctgtgcttgc
    40201 accaaccacc gttgtgaccc gtcaggatat cgaccgctgg cagtcgacct cggtcaatga
    40261 tgtgctgcgc cgtcttccgg gcgtcgatat cacccaaaac ggcggttcag gtcagctctc
    40321 atctattttt attcgcggta caaatgccag tcatgtgttg gtgttaattg atggcgtacg
    40381 cctgaatctg gcgggggtga gtggttctgc cgaccttagc cagttcccta ttgcgcttgt
    40441 ccagcgtgtt gaatatatcc gtgggccacg ctccgccgtt tatggttccg atgcaatagg
    40501 cggggtggtg aatatcatca cgacgcgcga tgaacccgga acggaaattt cagcagggtg
    40561 gggaagcaat agttatcaga actatgatgt ctctacgcag caacaactgg gggataagac
    40621 acgggtaacg ctgttgggcg attatgccca tactcatggt tatgatgttg ttgcctatgg
    40681 taataccgga acgcaagcgc agacagataa cgatggtttt ttaagtaaaa cgctttatgg
    40741 cgcgctggag cataacttta ctgatgcctg gagcggcttt gtgcgcggct atggctatga
    40801 taaccgtacc aattatgacg cgtattattc tcccggttca ccgttgctcg atacccgtaa
    40861 actctatagc caaagttggg acgccgggct gcgctataac ggcgaactga ttaaatcaca
    40921 actcattacc agctatagcc atagcaaaga ttacaactac gatccccatt atggtcgtta
    40981 tgattcgtcg gcgacgctcg atgagatgaa gcaatacacc gtccagtggg caaacaatgt
    41041 catcgttggt cacggtagta ttggtgcggg tgtcgactgg cagaaacaga ctacgacgcc
    41101 gggtacaggt tatgttgaga atggatatga tcaacgtaat accggcatct atctgaccgg
    41161 gctgcaacaa gtcggcgatt ttacctttga aggcgcagca cgcagtgacg ataactcaca
    41221 gtttggtcgt catggaacct ggcaaaccag cgccggttgg gaattcatcg aaggttatcg
    41281 cttcattgct tcctacggga catcttataa ggcaccaaat ctggggcaac tgtatggctt
    41341 ctacggaaat ccgaatctgg acccggagaa aagcaaacag tgggaaggcg cgtttgaagg
    41401 cttaaccgct ggggtgaact ggcgtatttc cggatatcgt aacgatgtca gtgacttgat
    41461 cgattatgat gatcacaccc tgaaatatta caacgaaggg aaagcgcgga ttaagggcgt
    41521 cgaggcgacc gccaattttg ataccggacc actgacgcat actgtgagtt atgattatgt
    41581 cgatgcgcgc aatgcgatta ccgacacgcc gttgttacgc cgtgctaaac agcaggtgaa
    41641 ataccagctc gactggcagt tgtatgactt cgactggggt attacttatc agtatttagg
    41701 cactcgctat gataaggatt actcatctta tccttatcaa accgttaaaa tgggcggtgt
    41761 gagcttgtgg gatcttgcgg ttgcgtatcc ggtcacctct cacctgacag ttcgtggtaa
    41821 aatagccaac ctgttcgaca aagattatga gacagtctat ggctaccaaa ctgcaggacg
    41881 ggaatacacc ttgtctggca gctacacctt ctgaaccacg tcccaccgtg ctggtgtttg
    41941 actccggcgt cggtgggttg tcggtctatg acgagatccg gcatctctta ccggatctcc
    42001 attacattta tgctttcgat aacgtcgctt ttccgtatgg cgaaaaaagc gaagagttta
    42061 ttgttgagcg agtggtggca attgtcaccg cagtgcaaga acgttatccc cttgcgctgg
    42121 ctgtggtcgc ttgcaacacc gccagtaccg tttcacttcc tgcattacgc gaaaagttcg
    42181 acttcccggt tgttggtgtc gtgccggcga ttaaacctgc tgcacgtctg acggcaaatg
    42241 gcattgtcgg attactggca acccgcggaa ctgttaaacg ttcttatact catgagctga
    42301 tcgcgcgttt cgctaatgaa tgccagatag aaatgctggg ctcggcagag atggttgagt
    42361 tggctgaagc gaagctgcat ggcgaagatg tttctctgga tgcactaaaa cgtatcctac
    42421 gcccgtggtt aagaatgaaa gagccgccag ataccgttgt attgggttgc acccatttcc
    42481 ctctactaca agaagaactc ttacaggtgc tgccagaggg aacccggctg gtggattctg
    42541 gcgcagcgat tgctcgcaga acggcctggc tgttagaaca tgaagctccg gatgcaaaat
    42601 ctgccgatgc gaatattgcc ttttgtatgg caatgacgcc agaagctgaa caattattgc
    42661 ccgttttaca gcgttacggc ttcgaaacgc tcgaaaaact ggcagtttta ggctgatttg
    42721 gttgaatgtt gcgcggtcgg aaaattattt taaatttcct cttgtcaggc cggaataact
    42781 ccctataatg cgccaccact gacacggaac aatggcaaac acgccgccgg gtcagcgggg
    42841 ttctcctgag aattccgaca gagaaagcaa aaataaatgc ttgactctgt agcgggaaag
    42901 cgtattatgc acaccccgcg ccgctgagaa aaagc
//
LOCUS       DAZGAN020000029        42782 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGAN020000029 DAZGAN020000000
VERSION     DAZGAN020000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 42782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.029"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..1383)
                     /locus_tag="ACHKYO_RS09025"
                     /old_locus_tag="pgaptmp_001805"
                     /pseudo
     CDS             complement(1..1383)
                     /locus_tag="ACHKYO_RS09025"
                     /old_locus_tag="pgaptmp_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1380..2435)
                     /gene="ycjS"
                     /locus_tag="ACHKYO_RS09030"
                     /old_locus_tag="pgaptmp_001806"
     CDS             complement(1380..2435)
                     /gene="ycjS"
                     /locus_tag="ACHKYO_RS09030"
                     /old_locus_tag="pgaptmp_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4428663.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(2445..3233)
                     /gene="ycjR"
                     /locus_tag="ACHKYO_RS09035"
                     /old_locus_tag="pgaptmp_001807"
     CDS             complement(2445..3233)
                     /gene="ycjR"
                     /locus_tag="ACHKYO_RS09035"
                     /old_locus_tag="pgaptmp_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGU4428664.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRTLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            complement(3251..4303)
                     /gene="ycjQ"
                     /locus_tag="ACHKYO_RS09040"
                     /old_locus_tag="pgaptmp_001808"
     CDS             complement(3251..4303)
                     /gene="ycjQ"
                     /locus_tag="ACHKYO_RS09040"
                     /old_locus_tag="pgaptmp_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HGU4428665.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(4334..5176)
                     /gene="ycjP"
                     /locus_tag="ACHKYO_RS09045"
                     /old_locus_tag="pgaptmp_001809"
     CDS             complement(4334..5176)
                     /gene="ycjP"
                     /locus_tag="ACHKYO_RS09045"
                     /old_locus_tag="pgaptmp_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU4428666.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(5163..6044)
                     /gene="ycjO"
                     /locus_tag="ACHKYO_RS09050"
                     /old_locus_tag="pgaptmp_001810"
     CDS             complement(5163..6044)
                     /gene="ycjO"
                     /locus_tag="ACHKYO_RS09050"
                     /old_locus_tag="pgaptmp_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU4428667.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(6065..7423)
                     /gene="ycjN"
                     /locus_tag="ACHKYO_RS09055"
                     /old_locus_tag="pgaptmp_001811"
     CDS             complement(6065..7423)
                     /gene="ycjN"
                     /locus_tag="ACHKYO_RS09055"
                     /old_locus_tag="pgaptmp_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4428668.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSTVKQVPVEEDAYNTKVITLS
                     RSGSLPEVIETSHDYAKVMDKEQLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAW
                     TGVPVSAWIGGIWYRKDVLAKAGLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESV
                     LTEQSFSQFALSNQANVFNAEGKITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDA
                     FMNGTVPMAIYSTYILPAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEET
                     EAAEKFVTFMEQADNIADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIG
                     ELPNIQVFGAVGDKNFTRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIE
                     QH"
     gene            complement(7437..9116)
                     /gene="ycjM"
                     /locus_tag="ACHKYO_RS09060"
                     /old_locus_tag="pgaptmp_001812"
     CDS             complement(7437..9116)
                     /gene="ycjM"
                     /locus_tag="ACHKYO_RS09060"
                     /old_locus_tag="pgaptmp_001812"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HGU4428669.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGKSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(9329..9643)
                     /gene="pspE"
                     /locus_tag="ACHKYO_RS09065"
                     /old_locus_tag="pgaptmp_001813"
     CDS             complement(9329..9643)
                     /gene="pspE"
                     /locus_tag="ACHKYO_RS09065"
                     /old_locus_tag="pgaptmp_001813"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HGU4428670.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(9718..9939)
                     /gene="pspD"
                     /locus_tag="ACHKYO_RS09070"
                     /old_locus_tag="pgaptmp_001814"
     CDS             complement(9718..9939)
                     /gene="pspD"
                     /locus_tag="ACHKYO_RS09070"
                     /old_locus_tag="pgaptmp_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HGU4428671.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(9948..10307)
                     /gene="pspC"
                     /locus_tag="ACHKYO_RS09075"
                     /old_locus_tag="pgaptmp_001815"
     CDS             complement(9948..10307)
                     /gene="pspC"
                     /locus_tag="ACHKYO_RS09075"
                     /old_locus_tag="pgaptmp_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HGU4428672.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(10307..10531)
                     /gene="pspB"
                     /locus_tag="ACHKYO_RS09080"
                     /old_locus_tag="pgaptmp_001816"
     CDS             complement(10307..10531)
                     /gene="pspB"
                     /locus_tag="ACHKYO_RS09080"
                     /old_locus_tag="pgaptmp_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HGU4428673.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(10585..11253)
                     /gene="pspA"
                     /locus_tag="ACHKYO_RS09085"
                     /old_locus_tag="pgaptmp_001817"
     CDS             complement(10585..11253)
                     /gene="pspA"
                     /locus_tag="ACHKYO_RS09085"
                     /old_locus_tag="pgaptmp_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HGU4428674.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            11420..12397
                     /gene="pspF"
                     /locus_tag="ACHKYO_RS09090"
                     /old_locus_tag="pgaptmp_001818"
     CDS             11420..12397
                     /gene="pspF"
                     /locus_tag="ACHKYO_RS09090"
                     /old_locus_tag="pgaptmp_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HGU4428675.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(12517..13782)
                     /gene="puuE"
                     /locus_tag="ACHKYO_RS09095"
                     /old_locus_tag="pgaptmp_001819"
     CDS             complement(12517..13782)
                     /gene="puuE"
                     /locus_tag="ACHKYO_RS09095"
                     /old_locus_tag="pgaptmp_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HGU4428676.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGKPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDVQFDAAMKILQDALSD"
     gene            complement(13820..15100)
                     /gene="puuB"
                     /locus_tag="ACHKYO_RS09100"
                     /old_locus_tag="pgaptmp_001820"
     CDS             complement(13820..15100)
                     /gene="puuB"
                     /locus_tag="ACHKYO_RS09100"
                     /old_locus_tag="pgaptmp_001820"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HGU4428677.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(15102..16589)
                     /gene="puuC"
                     /locus_tag="ACHKYO_RS09105"
                     /old_locus_tag="pgaptmp_001821"
     CDS             complement(15102..16589)
                     /gene="puuC"
                     /locus_tag="ACHKYO_RS09105"
                     /old_locus_tag="pgaptmp_001821"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HGU4428678.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANLV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(16864..17421)
                     /gene="puuR"
                     /locus_tag="ACHKYO_RS09110"
                     /old_locus_tag="pgaptmp_001822"
     CDS             complement(16864..17421)
                     /gene="puuR"
                     /locus_tag="ACHKYO_RS09110"
                     /old_locus_tag="pgaptmp_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HGU4428679.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(17448..18212)
                     /gene="puuD"
                     /locus_tag="ACHKYO_RS09115"
                     /old_locus_tag="pgaptmp_001823"
     CDS             complement(17448..18212)
                     /gene="puuD"
                     /locus_tag="ACHKYO_RS09115"
                     /old_locus_tag="pgaptmp_001823"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HGU4428680.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            18424..19842
                     /gene="puuA"
                     /locus_tag="ACHKYO_RS09120"
                     /old_locus_tag="pgaptmp_001824"
     CDS             18424..19842
                     /gene="puuA"
                     /locus_tag="ACHKYO_RS09120"
                     /old_locus_tag="pgaptmp_001824"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HGU4428681.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            19991..20155
                     /locus_tag="ACHKYO_RS09125"
                     /old_locus_tag="pgaptmp_001825"
     CDS             19991..20155
                     /locus_tag="ACHKYO_RS09125"
                     /old_locus_tag="pgaptmp_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4428682.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRLTRPQTL
                     LRRGGSNYGY"
     gene            20145..21530
                     /gene="puuP"
                     /locus_tag="ACHKYO_RS09130"
                     /old_locus_tag="pgaptmp_001826"
     CDS             20145..21530
                     /gene="puuP"
                     /locus_tag="ACHKYO_RS09130"
                     /old_locus_tag="pgaptmp_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HGU4428683.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKEHFHYLLMPLVGALTVGVLWINLEATSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            21664..21909
                     /gene="ymjA"
                     /locus_tag="ACHKYO_RS09135"
                     /old_locus_tag="pgaptmp_001827"
     CDS             21664..21909
                     /gene="ymjA"
                     /locus_tag="ACHKYO_RS09135"
                     /old_locus_tag="pgaptmp_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HGU4428684.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            22222..23865
                     /gene="sapA"
                     /locus_tag="ACHKYO_RS09140"
                     /old_locus_tag="pgaptmp_001828"
     CDS             22222..23865
                     /gene="sapA"
                     /locus_tag="ACHKYO_RS09140"
                     /old_locus_tag="pgaptmp_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HGU4428685.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWCGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            23862..24827
                     /gene="sapB"
                     /locus_tag="ACHKYO_RS09145"
                     /old_locus_tag="pgaptmp_001829"
     CDS             23862..24827
                     /gene="sapB"
                     /locus_tag="ACHKYO_RS09145"
                     /old_locus_tag="pgaptmp_001829"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HGU4428686.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            24814..25704
                     /gene="sapC"
                     /locus_tag="ACHKYO_RS09150"
                     /old_locus_tag="pgaptmp_001830"
     CDS             24814..25704
                     /gene="sapC"
                     /locus_tag="ACHKYO_RS09150"
                     /old_locus_tag="pgaptmp_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HGU4428687.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            25704..26696
                     /gene="sapD"
                     /locus_tag="ACHKYO_RS09155"
                     /old_locus_tag="pgaptmp_001831"
     CDS             25704..26696
                     /gene="sapD"
                     /locus_tag="ACHKYO_RS09155"
                     /old_locus_tag="pgaptmp_001831"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HGU4428688.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            26698..27504
                     /gene="sapF"
                     /locus_tag="ACHKYO_RS09160"
                     /old_locus_tag="pgaptmp_001832"
     CDS             26698..27504
                     /gene="sapF"
                     /locus_tag="ACHKYO_RS09160"
                     /old_locus_tag="pgaptmp_001832"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HGU4428689.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            27572..27925
                     /gene="ycjD"
                     /locus_tag="ACHKYO_RS09165"
                     /old_locus_tag="pgaptmp_001833"
     CDS             27572..27925
                     /gene="ycjD"
                     /locus_tag="ACHKYO_RS09165"
                     /old_locus_tag="pgaptmp_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HGU4428690.1"
                     /translation="MMDKIKSNARDLRSNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            28293..29081
                     /gene="fabI"
                     /locus_tag="ACHKYO_RS09170"
                     /old_locus_tag="pgaptmp_001834"
     CDS             28293..29081
                     /gene="fabI"
                     /locus_tag="ACHKYO_RS09170"
                     /old_locus_tag="pgaptmp_001834"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HGU4428691.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            29226..30353
                     /gene="yciW"
                     /locus_tag="ACHKYO_RS09175"
                     /old_locus_tag="pgaptmp_001835"
     CDS             29226..30353
                     /gene="yciW"
                     /locus_tag="ACHKYO_RS09175"
                     /old_locus_tag="pgaptmp_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HGU4428692.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            30421..32355
                     /gene="rnb"
                     /locus_tag="ACHKYO_RS09180"
                     /old_locus_tag="pgaptmp_001836"
     CDS             30421..32355
                     /gene="rnb"
                     /locus_tag="ACHKYO_RS09180"
                     /old_locus_tag="pgaptmp_001836"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HGU4428693.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(32433..32537)
                     /locus_tag="ACHKYO_RS09185"
                     /old_locus_tag="pgaptmp_001837"
     CDS             complement(32433..32537)
                     /locus_tag="ACHKYO_RS09185"
                     /old_locus_tag="pgaptmp_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4428694.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            32591..34576
                     /gene="pdeR"
                     /locus_tag="ACHKYO_RS09190"
                     /old_locus_tag="pgaptmp_001838"
     CDS             32591..34576
                     /gene="pdeR"
                     /locus_tag="ACHKYO_RS09190"
                     /old_locus_tag="pgaptmp_001838"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGU4428695.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(34558..34686)
                     /gene="ymiD"
                     /locus_tag="ACHKYO_RS09195"
                     /old_locus_tag="pgaptmp_001839"
     CDS             complement(34558..34686)
                     /gene="ymiD"
                     /locus_tag="ACHKYO_RS09195"
                     /old_locus_tag="pgaptmp_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HGU4428696.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            34723..34896
                     /gene="yciZ"
                     /locus_tag="ACHKYO_RS09200"
                     /old_locus_tag="pgaptmp_001840"
     CDS             34723..34896
                     /gene="yciZ"
                     /locus_tag="ACHKYO_RS09200"
                     /old_locus_tag="pgaptmp_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HGU4428697.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            34986..35735
                     /gene="yciT"
                     /locus_tag="ACHKYO_RS09205"
                     /old_locus_tag="pgaptmp_001841"
     CDS             34986..35735
                     /gene="yciT"
                     /locus_tag="ACHKYO_RS09205"
                     /old_locus_tag="pgaptmp_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HGU4428698.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            36004..36222
                     /gene="osmB"
                     /locus_tag="ACHKYO_RS09210"
                     /old_locus_tag="pgaptmp_001842"
     CDS             36004..36222
                     /gene="osmB"
                     /locus_tag="ACHKYO_RS09210"
                     /old_locus_tag="pgaptmp_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HGU4428699.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(36348..36674)
                     /gene="yciH"
                     /locus_tag="ACHKYO_RS09215"
                     /old_locus_tag="pgaptmp_001843"
     CDS             complement(36348..36674)
                     /gene="yciH"
                     /locus_tag="ACHKYO_RS09215"
                     /old_locus_tag="pgaptmp_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HGU4428700.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(36674..37411)
                     /gene="pyrF"
                     /locus_tag="ACHKYO_RS09220"
                     /old_locus_tag="pgaptmp_001844"
     CDS             complement(36674..37411)
                     /gene="pyrF"
                     /locus_tag="ACHKYO_RS09220"
                     /old_locus_tag="pgaptmp_001844"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGU4428701.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(37604..38773)
                     /gene="lapB"
                     /locus_tag="ACHKYO_RS09225"
                     /old_locus_tag="pgaptmp_001845"
     CDS             complement(37604..38773)
                     /gene="lapB"
                     /locus_tag="ACHKYO_RS09225"
                     /old_locus_tag="pgaptmp_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HGU4428702.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(38780..39088)
                     /gene="lapA"
                     /locus_tag="ACHKYO_RS09230"
                     /old_locus_tag="pgaptmp_001846"
     CDS             complement(38780..39088)
                     /gene="lapA"
                     /locus_tag="ACHKYO_RS09230"
                     /old_locus_tag="pgaptmp_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HGU4428703.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(39237..40001)
                     /gene="pgpB"
                     /locus_tag="ACHKYO_RS09235"
                     /old_locus_tag="pgaptmp_001847"
     CDS             complement(39237..40001)
                     /gene="pgpB"
                     /locus_tag="ACHKYO_RS09235"
                     /old_locus_tag="pgaptmp_001847"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HGU4428704.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            40171..40761
                     /gene="ribA"
                     /locus_tag="ACHKYO_RS09240"
                     /old_locus_tag="pgaptmp_001848"
     CDS             40171..40761
                     /gene="ribA"
                     /locus_tag="ACHKYO_RS09240"
                     /old_locus_tag="pgaptmp_001848"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HGU4428705.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(40825..>42782)
                     /gene="acnA"
                     /locus_tag="ACHKYO_RS09245"
                     /old_locus_tag="pgaptmp_001849"
     CDS             complement(40825..>42782)
                     /gene="acnA"
                     /locus_tag="ACHKYO_RS09245"
                     /old_locus_tag="pgaptmp_001849"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HGU4428706.1"
                     /translation="PVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKF
                     VEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEK
                     YAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELE
                     VNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLG
                     LKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIE
                     TAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEPIG
                     HDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTYG
                     WQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAG
                     RYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVV
                     SIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNL
                     IGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCR
                     CRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        10078 a        11283 c        10789 g        10632 t
ORIGIN      
        1 ttcgccaggt taatcgccgg cttagccata aacgaatcat cctgcggcaa aacgccgtcg
       61 ggctgaattt ctggcagccg cagttcttta aggaacattt cggcacgatg aataaacgcg
      121 tcgtcgctac agccaaactg gcgggcaata ctcagcgcct gctggacgtt gtaatacgcc
      181 atatagctgg tgaaggcgtt attattgaca tgttcggtat attcgtcagg cccaataaca
      241 tcatgaattt ccagacggtc gttaaccctc accgcgcggc taatccagaa ctttgcagtt
      301 tccagaagta gcgccatgcc ttcatgcgca atgaaacttt catcccccgt ggtctgccag
      361 tattgaataa ccgcccaggc gatatcggcc accagatgat gttccgcctg cgccgaggcc
      421 actttttgcc gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata
      481 aagcgcataa tctaacgctt gctgatcgtg cgcttcgccg ccatttaccg tgatacgacg
      541 tttctgccac cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg
      601 ctgcgcgcac atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg
      661 cctgtcatcc cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg
      721 aagctgcgca ctggtatgtt gctgcaaacg gcgctctttg gcggtatagc attgctgcac
      781 atcaccgctc accatacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt
      841 gtagatcccc tgcatcagat gctgaccaaa cacccgcacc tgggtttcgt cgagatgttg
      901 gcgaccgtgg ttggtttgcg tggcgtcgat acctgttgaa atcagcactg acgcgtcggc
      961 gtccagtggc gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa
     1021 gcgacggctg gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg
     1081 taattctcca ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac
     1141 ctcaccattt atggcaatct ccatccccaa gatatcaggc aggttcacca gttcgttgat
     1201 ttcacctttt cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt
     1261 gtaatcttct tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga
     1321 tgcatacttg ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt
     1381 cataattcca cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg
     1441 atgatgtacc cctgctcggc gtcggctatc atcacgggct tgccctgtac gtgattgata
     1501 aaggcttcca tgctgcgcaa atggcggttg tcgtctgcca gttcccgttg catcagcgtc
     1561 attaattcac cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accggcttta
     1621 tcaccacaaa agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt
     1681 tccagccaca gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg
     1741 ctataagttg ccggatccca ctcgccaaat tgaccacagc tcttttgcgt gccgatcttt
     1801 tgaaagctat gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca
     1861 tccagcatat gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta
     1921 aagacgcccc agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc
     1981 tctcccaaaa cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga
     2041 tggtgaaagt cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc
     2101 gcctgttctg gcgtcatggc gggcggtttt tcgcacatca catggcagcc cgcttccagt
     2161 gccatcaggg tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt
     2221 ttcaccgcca gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct
     2281 gccagcgcct gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg
     2341 cgggtgcagt agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg
     2401 acccgcagtg gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac
     2461 caggtcaacg aatcacggta cgcctgggca ggatcttctg cccggatacg cccttcatac
     2521 accacataac cctgatagtt atcggcgcgc agctgttcaa acagcgcgtg gaaatcgagg
     2581 gtgccgctgc ccggctggta acgatggtta tcggcaatat gcacatgacc gagcaggtcg
     2641 cggttgtcgt gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg
     2701 ccgataatct gcacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgaga
     2761 gtgttgatca tatgatcctg atagcggttt aacggttcga gatacaccac ggttccggta
     2821 cgcgctgcca cttgttccag cgtgcgcagg gaatcgctca ccattttacg atcgccgtcc
     2881 aggctgcgcg gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga
     2941 acgacgatac ctttaccccc cacttctgcc agcgcttcaa gaatgcgttc gatctgcttt
     3001 aagccattaa gacgacgctc ttcgataaag tcaccaatcc agccgtcata gccaccgcag
     3061 gcggtggtca ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg
     3121 accagcagtt tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat
     3181 ttctccagaa tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca
     3241 ttcctttgaa ttaaaacgta acgcccattt tgatgctctg ttccggatgc tggtcgacat
     3301 actgcatgta gctttccggg ctattggcaa aggtcaccac cgggtcgatt agatcttcac
     3361 aattgagata accgttcatc agcagttccc aacaggtttc ttcgatacgc ttgcggctcc
     3421 agcgcggata atccgggttc ggttcgctgc acgcgcggga gaagacaatt ttggcgttat
     3481 tgaaatgggc ttcgcgtccg aggttaaaac cttcggcaaa cggtttggca aacgcaacat
     3541 acgagatggt gccgccataa gccaggccgc ggagcgccga ttgcagcgcg tcggcgtaac
     3601 cgctggtttc gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttac
     3661 ctacgtcagt gccaatgggg ttaaggcaga aatcagcacc gtggcgacgg gcaatatcac
     3721 agcgataggc gataggatcg acgccaatca ccaccgacgc gccagcgcgt ttcgccagtt
     3781 ggatggctat ttgaccaatc gcgccaagcc ctaccaccac cacaaaatca cccacgcgca
     3841 cgttggcatc gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacag acggcgtttt
     3901 tccaggagct gccttgcggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga
     3961 ccgtctcgga gagtgggccg tagccgcata cgctgtcgcc caccgcgtag tcggttacgt
     4021 cgctgccgca ctcgataatg tcgccaacca ccatgttgcc aagctggaat ttgccaaact
     4081 caatgccgcg cggcgcatct gccggacgcg gagtgaacat ctgccattca ccgttaaagt
     4141 cttcatcgat aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg
     4201 cgccgaaacg cacgcggatc ttaacttcat tggctaaaat cgcccgatct tcatactcaa
     4261 ccagcgcagc aacgcgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa
     4321 gaaactggcc gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt
     4381 ccgaaagcgc atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc
     4441 gtccccagat gtaatctggc gtactgaaca gtgcgttcag gcccaccggt aaggtgaaat
     4501 tgctggcgct ggaaaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc
     4561 agtagacgaa cacggatatc agcccggaca tcgccagggg cacggtgata cggaagatga
     4621 tttgcagacg gttgaggcca tccatcatcg ccgcttcttc gatctcatca gggatggtgt
     4681 cgaagtagct tttcagcata aacacggcgg tgggcagggt ttgcgtcacc atggtaatga
     4741 tcagcgccat ctcggtgtcg taaatgccca gcgcggtgat gattttgaac agcggcacca
     4801 ccagcaaaat gccagagaac atatacaccg tgtaaaagct ggcattgatg gtcatccgac
     4861 ctttaaagcg caggcgggaa agcgcgtaag ccccaagaat gccgaggaat accgccacca
     4921 cggatgaaac caccgacacc accagactgt tacggaagta gtcgacaaac gggaaaatca
     4981 tcgggttaaa aatgtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat
     5041 gcagtgagat cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa
     5101 acagcgtgat gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc
     5161 gtttatttgt tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata
     5221 atgatgaaga gcaccaccga gatcgccgcc gcttttccta aatcattaaa ggcaaaggcg
     5281 gttttgtaga gatatacacc gagaatatcg accttggtcg tcagcaaata aacatccgcg
     5341 aacatgtaga acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatc
     5401 gcgggcagcg tgacgatacg aaaccgttgc caggcattag cgccatccat ctctgccgct
     5461 tcgtacagcg atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg
     5521 aagtagcgcc agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac
     5581 cacagcggtg cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg
     5641 tagccgttgt tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag
     5701 gagaggatca ccagcgaacg cgcggttttg cgcagacgga actcgcggtt gaaaaacatc
     5761 gccactgcca gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc
     5821 atccacagcg agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca
     5881 acaaaggttg actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc
     5941 gacaccattg gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc
     6001 agcgcaaagg gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct
     6061 gggtttagtg ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag
     6121 ggagatcggc tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaccccg
     6181 tcacatcacc catgcgggta aagtttttat cccctactgc accaaagacc tgaatatttg
     6241 gcagttcacc gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt
     6301 tccaggtggc ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca
     6361 tcacccagtc ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt
     6421 ccgtctcttc ggtcttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg
     6481 cagagttttt ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatca
     6541 cagccggaag gatataggtg gagtaaatcg ccatcggcac ggtgccgttc ataaaggcgt
     6601 ctttcacttc catgatgtcg ttagaacccg gcatagtgtt ggcagcaagg tcgcggtaat
     6661 aggtcagtgc ctgcatcatc tctggtgtat caagggtgat tttgccttcg gcgttaaaga
     6721 cgttagcctg gttggataac gcaaactggg agaaggattg ttccgtcaac acgctttctg
     6781 ctgtaggcag cgcaatgccg tattttttat tcgccgggtc attcagtttc tgtgcaacgt
     6841 ccagcagctg ttgccagttt ttcggctcct caagccccgc ttttgccagc acatctttgc
     6901 gataccagat accgccaatc caggcgctga caggaacacc ggtccatgcg ctaccatctt
     6961 cggtacgcac aatacgcagt acgccatcgt aaaacgcgcc ttcaccaacg ttgctgatga
     7021 ctgtggcaac cgctttgcga tcgataagct gctctttgtc catcactttg gcgtagtcat
     7081 ggctggtttc tatcacttcc ggcagcgaac cgctacgtga aagagtaatg actttagtgt
     7141 tataggcatc ttcttccacg ggcacttgct taacggtgct acgtgaaaga gtaatgactt
     7201 tagtgttata ggcatcttct tccacgggca cttgcttaac ggtgatgcca gggttttctt
     7261 tttcaaaacg ggcaatcaat ttactgataa cggcctggcg ctcctgctcc accgaagaat
     7321 gcataaattc gatggataca ttcgttttat tttcttcttt acagcctgaa atcagggcgc
     7381 aggaaaccag tgctgataac agcacgattt ttgatttaat catgagatgt tcctttttat
     7441 ttaatccaca taacctgcca ggggcgcagc gttatttcat tacccaatat atcaacttca
     7501 ctaatcagat cccgaccatg cagattagta atatttacat gctgaatatt tttactgaca
     7561 ttaaacaatc cagtcagaca attaccatca gcgttacttc tttgaatacg cattacggat
     7621 gaattaatcg tatcgatggt aaaattatta tccggatgaa actcgttatg gctgcgacga
     7681 agtgtaatta aacgcgacaa ctcatgatat accgcatgcc ttaatgtagc ttcatcgttc
     7741 agttctcggg ttatctcttt actgtgatat tttttacggt taatcgcacg gttatatccg
     7801 agtttttcga cacctgcgta atcattacgc gagccaagaa tactttgaat atatatcgct
     7861 ggcacaccgg ggaaacttaa caaaatcgca tgggcgagaa taaacctggc gcaacgttct
     7921 tcatcgctac tctcacggcg gcttaacgca tccatatagg tcacatttat ttcatacgga
     7981 ctgcgtgtac cgtcgggatt atttttccag tttactaatg caccttcctg ctgtaacgcc
     8041 tcgaccagct ctaatatttc gctttcaggc aacaagcccc gtagcgggtt tagcccgatg
     8101 ccatcgtgag aggcgaggaa gttaaaccag gtggtgttgc tggaaggtag tgtcaggttt
     8161 tgcgcccacg cacaaagcgc ctcaacgttc tgtttttgca ccgcatgcag caccagtggc
     8221 ggcagcgaga actggtacac catatgtgct tcgtcatcgc ctgcgccaaa gtaagcaatg
     8281 ttgtctttat gcggaacatt ggtctcggta atgatcactg tacctggcgc aacgttatca
     8341 ataatcgacc gtaacagttt gataatcaga tgtgtttttt ccagatggat gcagcttttt
     8401 cccggctctt tccacataaa gccaacggca tccaggcgga catactcagc acctttcgca
     8461 aggtaacaga gtaaaacatc caccatcgcc agcaacactt cagggctacg gtaattcagg
     8521 tcaatttgat cgtcactaaa ggtggtccat aaatggcgcg ttgaatgatc gcgcatctgg
     8581 aatggcgtta ataacggtaa cgcacgcggg cgagtgacgg cgctgagatc ggtttgcggg
     8641 tcaacggcaa taaaaaaatc ttcaaaacct ggatgctgtt gtaaatagtt tttaaaccat
     8701 tcactttttg ccgacatatg gttgcagaca aaatcaaaca ttaaatgact gcattcaccg
     8761 agttgctgaa tatcctgcca ctcccccgct tcactggcga cctgatgata atcaattacc
     8821 gaaaagccat catcagatga ccatggataa aacggcaaca aatgaacatg tgaaaaaatg
     8881 ctttgcagcc attgatggta aaactgatta aatgtgggta atggttttaa atcattgctg
     8941 tgaaattgat cggcataggt aattaacacg acatcacttt catcccagtg ttttttacgt
     9001 cgctgtgtaa ttaatcgttt cgcactctca agacgcgtta caagtttctg taaatgagtt
     9061 gcggtaaatg ttccaccgta gatttcgtcc aggtaatccg taattttctg tttcatgttg
     9121 cctttccgtg gtagcggtcc catgaaacga gagactactc cgcagaaaat aaactgtcaa
     9181 ttgagcacaa gggttttagt cacgcaggaa atcatttttt accagggaaa aagcgtattt
     9241 gtgagctgac gcaaataatc tatggggatg taaataaggt gtgacagaaa aaaaaacggc
     9301 gcataagcgc cgctcatggt gaattctttt aacctttgac cttcggcatt gcgatgtctt
     9361 tcaggccacc ggcgttctca acgtgggtat atcccatctc gctaaggatc tcttttgctt
     9421 gccctgactg gcgtccggca ttgcaataca ctttcacggt gtcgttttta tccggaacgg
     9481 cggtggcaat gcgctctttc acttccttca ggggaatatt gatggccccc tgaacgtgct
     9541 cttgctgata ctgctctgga acacgaacat cgatccagtg ttcagcggcg aaaacgggca
     9601 gtgaaaacac cagtgccaga gcaagtaagc cttttttaaa catgtaagcg tccttctatg
     9661 gatgaaaatt gccatccatt atacgcctga gatttaacaa tttttgcgaa actcccctca
     9721 ccttttataa cgctgtgcca gtttattagc agcccgactt aacagcggtt ccagcgccac
     9781 agccagcaac attttcagcg gtcggcgagc aactgatttt atcgcccagc ccgctacacc
     9841 cgccgggcca tagcgcagtg cggtaagaag taccagcttg cctgctaatt tgaaaccagg
     9901 ctttaccttt tgcccggcct gttgccagcg agtattcata actttcctca cagttgacgg
     9961 aaacggctac gtaacgtgaa agtatcggaa gtgacataac gttccatctc gcgtaaacgc
    10021 gtttcacttg ccgccagttc acggtcgact tcatccagca attcgctgct ggaaggtagc
    10081 tgctcaccaa aggccatgtt gtccggcatt ggatcaagcg caaatgacaa aatgatgtaa
    10141 gcaaccaggg taaacagcgc cagaccgaag aaaatcgaca gcaccaccag gatacgcacc
    10201 agttttgccg gtacatcaaa atagttggca atcccggcgc agacgccgcg gaccatgccc
    10261 tgctgtggaa tacgccataa ttttttattg agattaatgc ccgccattag cgatccctcc
    10321 agttcggatg ttctgcatca agaatagatt ccagcgcctg aatacgttcg cgcatccgtt
    10381 ttgcttcatc agccagttgc gctaatcgct gctgctcact ttgcgacaat tcactgcgac
    10441 cagaacgatt gctgtaatgc agccataacc agatcggtaa aacaaacagc acaaaaatgg
    10501 ttaacggaat agccagaaat agcgcgctca taagtactcc ttagacgatg agcggcggcg
    10561 cgttcggacg ccgcctggat gttattattg attgtcttgc ttcattttgg cttttaattg
    10621 tgccagttgt tcgctgattg catcatcggc tttcagttcg gcaaactgat cgtccagcga
    10681 tttttgttta ccgaagctgt ggctttctgc ttccgcttcc atctggtcaa tacgacgttc
    10741 gaaagattcg aaacgagcca ttgcttcatc cagtttgcca ctgtccagct gacgacgcac
    10801 atcgcgcgac gagtttgccg cctgatgacg taacatcaat gcctgctggc gagcgcgtgt
    10861 ttcgctcaat ttgttttcca gctcaccgat ctctttcttc atgcgtgcca gcgtgtcgtc
    10921 caccagcgtc acttcatgtt ccaggctctt aatcagatcg gtcagtttct gtttttcaat
    10981 taacgctgca cgtgccagat cctctctctc tttcagcagc gccagttcgg ctttttcctg
    11041 ccattcaact tcacgcgccg acgcttgttc aatacggcga gtcagctgtt tcttttctgc
    11101 caacgcgcgc gccgaagtag aacgtacttc aaccagtgta tcttccatct cctggatcat
    11161 cagacgaacc agtttctgtg gatcttccgc tttctctaac agagcgttga tgttggcatt
    11221 cacgatgtcg gcaaagcgag aaaaaatacc cataatgttg tcctcttgat ttctgcgttc
    11281 aggattgtcc tgctgaactg ttaatacaat ttgcgtgcca attttttatc tttttgattt
    11341 ataaagatct gattgaagaa tcaacagcaa catgccagga tgagttagcg aattacacta
    11401 acaagtggcg aatttcatca tggcagaata caaagataat ttacttggtg aggcgaacag
    11461 ctttctcgaa gtgctggaac aggtttcgca tctcgcaccg ctggacaaac cggtgctcat
    11521 catcggcgaa cgcggcaccg gtaaagagct gattgccagc cgcctgcatt atctctcctc
    11581 ccgttggcaa gggccgttta tttcccttaa ctgcgcggcg ttaaatgaaa atctgctgga
    11641 ttccgaactg tttggtcacg aagcgggggc gtttaccggt gcgcaaaaac gtcatcctgg
    11701 gagatttgaa cgtgccgacg gcggtacgct atttcttgat gaactcgcta cggcaccgat
    11761 gatggtgcag gagaaattat tgcgtgtgat tgagtacggt gaactggagc gcgttggcgg
    11821 cagccagcca ttgcaggtga atgtgcggtt ggtatgcgcg acgaatgccg atctcccggc
    11881 gatggtcaat gaaggcactt ttcgcgctga cctgctcgac cgactggctt ttgatgttgt
    11941 acaactgcca ccactgcgcg agcgcgaaag cgacataatg ttgatggcag aacactttgc
    12001 catccagatg tgtcgggaaa tcaagctgcc tctgttcccg gggtttacgg agcgcgccag
    12061 agaaacattg ctgaattatc gttggccggg aaatattcgt gaattgaaaa acgtggtgga
    12121 acgttcagtg tatcgccacg gcaccagcga ttatccgctt gatgacatca ttattgatcc
    12181 ctttaaacgg cgtccgcctg aagacgctat cgccgtttca gaaaccacct cgcttccaac
    12241 actgccgctg gatttacgtg agtttcagat gcagcaggaa aaagagttgc tgcaactcag
    12301 tttgcaacaa gggaaatata accagaagcg cgcggctgaa ttactggggt taacttatca
    12361 tcagttccgc gcgttgttga aaaagcacca gatttagcga agttgagcaa tacaacttgc
    12421 aggatgcggc gtgaatactt tattcggcat acataatcaa tacgtcgttt gcaggcctga
    12481 taagcgcagc gcatcagaca ttattgcgtt gggctattaa tcgctcagcg catcctgcaa
    12541 aattttcatt gccgcatcga attgcacatc cgggatggtc agcggataca ggaagcgaat
    12601 cacgttgccg tatgcgccac aggtcagcag gagcagcccc tgcgccagcg cgcgttgctg
    12661 gattttctgt gcaatcgccg ctgacggctt gcccgtttgc ggatcgttaa actctgccgc
    12721 aatcatcgac cccaggccgc gtaccgcagc aatggccgga acgctttctt tggcatcaat
    12781 caacgtgttt ttgagacgct ggcccagttg attcgcgcgt tcgcagagtg attctttgtc
    12841 gataatattg agcaccgcgt gcgcggcagc caccgccagc gggttcccgg cgtaggtgcc
    12901 acccaacccg cccggcgcgg gcgcgtccat aatattcgcg ttaccgacca cgcccgaaag
    12961 cggcatcccg cccgcgaggc ttttcgccat cgtcattaag tccggcttat cggcgtaatg
    13021 atccatggca aacagtttac cggtacgcgc aaagccgctt tgcacttcat cggcaatcat
    13081 cacaataccg tgctcgtcgc acaggcggcg aatggcggca accagctctt ttggcgcaac
    13141 gttgaaaccg ccctcgccct gcaccggttc gaaaataatg gccgcgacct gcttcgcttc
    13201 gatgtctgat ttaaacaagc gttcgatggc gtcgagggag tcctgtgttg aaatgccgtg
    13261 taaatctgac ggataaggta cgtgatacac cgaaccaggg aacgggccga agccgatttt
    13321 gtacggcgca acttttccgg tcagcgccat ggtcatatat gtacgaccgt ggaagccacc
    13381 gctaaacgca atcacgccag gtcgtccggt atgggcgcga gcaattttca ccgcgttttc
    13441 caccgcttcc gcaccggtag tgaagaatgc ggttttagcc tgcccgctca ctggggcaag
    13501 ggcgttaatt ttctccgcca gcgtgacgta gctttcgtac ggcacaatct gatacgccgt
    13561 gtgggtaaac tgttgcaatt gctgctccac cgccgcgacc agatcagggt ggcgatgccc
    13621 ggtattcagc accgcaatgc ctgcggcgaa atcgatgtat tcgttgccct ctacgtcctt
    13681 cagcgtagcg ttttctgccg actgggcgaa gaagttacac atcacgccaa ccccgcgtgg
    13741 ggtagcagaa agacgacgct gatggaattc attgttgctc atgtttcaca ccttttgact
    13801 cttcactgtt aatcggaaat taaacgccca gacgatcgcg caggctgtaa taagccgcgc
    13861 ccatcgcggt aaatggcacg cgcagcgtgc gcccgccggg gaacgggtaa tgcggcagat
    13921 tggcgaaggc atcgaaacgt tcggcgtcgc cgcgcagcag ttcggcaatc agacgcccgg
    13981 caagatgggt acaggtgacg ccgtggccgc tgtagccctg catgtaatag atgttggtat
    14041 caaggcgacc gaactgcggc atgcgcgaca aggtcagcag gaagttgccc gtccagcggt
    14101 aatcaatttt cacgcccttc agctgcggga aggttttcag cagtttcggc accacaaggc
    14161 gctcaacgtc atccgggtcg cgcgcgccgt agaccacgcc gccgccgtac agcaggcggt
    14221 tgtcggcggt aagacggtag taatcaagca gatagttaca gtcttccaca cagtagtttt
    14281 tcgggatcag cgaacgggct aaatcttccg acagccgttc ggtggtgatc acctgcgtgc
    14341 cgcacggcat gctgcgtttc gccagttccg gctctacttt atcgcccaga tacgcattcc
    14401 cggcgacaat cacatacttc gccgtcacct gacctttggc agttcgcaca acggctggcg
    14461 tggtgtgctg gatctgcgtc acggcagaaa gttcatacac gcgcccgccg ttgaggcgga
    14521 tggcgtccgc ttcgccaatg gcgaggttta gcggatgaat atgcccaccg ctgtgatcca
    14581 gcagcgcacc ggtatagcga tcgctggcta cttcacggcg aatggcgttg gcgtccagca
    14641 attccagctg tttattgccg tagcgttccc agttctcttt ctgctcttca agtgtggcga
    14701 gctgtttatc attcatcgcc acaaacaggc cgccggggcg gtagtcgcag tcaatctgat
    14761 aacgtttgat acgttcgcgg atgatctcac caccttcgaa catcatgctg ccgagcatac
    14821 gggcggtgtc catgccgtag cttttttcga tcacgtcgat gtcgcggctg taggagttca
    14881 caagctgccc gccattgcgc ccgcttgcgc caaagccgat gcgtgaggct tcgagaacca
    14941 ctacgtcaaa gcccgcttcc gccagatgca gcgcggaaga gagcccggta tagccgccgc
    15001 cgaccacgca aacgtcgcag gaaatcgact cattcagcgt gtcgaatggc gcgtatttat
    15061 tcgcactggc ggcgtagtaa ctgctggtat gttcggtcat ttcaggcctc caggcttatc
    15121 cagatggttt tcagttcggt gaatttttca agggcatgca gggatttgtc gcgaccgttg
    15181 ccgctctgct tatagccgcc aaacggcacg gtcatatcgc cgtcgttgta gttattgacg
    15241 aagacggaac cagccttcag gcggcggctc atgcggtgcg cgcgggagag gtcgcgcgtc
    15301 cataccgccg cgccaaggcc gtactggctg tcgttggcaa gctgtagcgc ctggtcttct
    15361 gatgtgaaac gcgtgaccac cagcaccgga ccgaaaatct cttcgcggct taaggacgca
    15421 ttcgggtcta catccacaaa gatggtcggg ccgatggcgg cagccagctc agcgttacga
    15481 ccatccagca gcagttgccc tttgctttcg ccttcccgaa taaagctatg gaccgagtcg
    15541 gcgtgggcgc agtcgattaa ggtgcccatg gtggttgcgg gatcaagtgg atggcccggc
    15601 tgccagtttt gcgcctgctg ttttaacagg gctaagaatt catcggcgat gctctcttcc
    15661 agcaacaaac gcgttccggc gatgcacacc tggccctggt tgtagaagat gcctgcggcg
    15721 gtggcgcttg ccgctttttg cagatccgga caatcggcga aaacgaggtt ggcgcttttg
    15781 ccgcccgctt ccagccagac gcgtttcatg ttgctgtcgc ccgcatcttt cagcagctgt
    15841 ttcccggtac gggttgaacc ggtaaaggca atggcgtcga tatcgttatg acgcgacagc
    15901 gcctgccccg cttcatgacc aaaacccgtc accacgttca acacaccatc cggcaagcct
    15961 gcttctttcg ccagccccgc gagacgaatc gcactgagcg gtgatttttc agacggtttt
    16021 agaatcacgc tgtttcccgc cgccagcgcc gggccgagtt tccagcaagt cagcaacagc
    16081 gggaagttcc acggcacgat ggcggcaatc acgccgaccg gttcacgcac gatcatcgcc
    16141 agctcatggc tactggtggt cgccacttcg ccatacactt tgtcgatcgc ttcggcgtac
    16201 cagcgaatgg cgcgcgccgc gccgggaata tcatcacgca gactgtgacg aatcggtttg
    16261 ccggtgtcga gagtttccag cagtgccagc tcttcggcgt gggcttccat taaatcggcg
    16321 agtttattca gtaccgcttt acgtttagcc ggagaagaca gtgaccagtc gccgcgttca
    16381 aatacgccgc gtgctgcgct caccgcacgg tcgatatcga cgctcttgcc gcgggcaatt
    16441 ttcgccagcg gtgcctgggt gaccggatca acggtttcaa aggtttcatt ttccgccgca
    16501 gcagtatatt caccgttaat aaataagcgg ttttcaatgg cgagacttaa cgctttatcc
    16561 tgccagtaag ccagatgatg aaaattcatt atgactcctg tttcacgtct atcagatata
    16621 tgcagtttgt gaacgtcagg gcgcagagat ccccgggccg tatgcccgtt tatttctggc
    16681 gacattcttt atatacactt catccttcaa gccgcctctg ccttggctgc gttcgctccc
    16741 ccgaatcact tacctgagta agctcatcgg gattcgctca cttgccgcct tgatgcaact
    16801 tgaatgattt tgtgtataga ttgccgggcg cattgcgctt acccggctta cagaacaaaa
    16861 agattaaaac gtggtgggcg tatgggcgct gataattcgg caaatacctg ccgacgtatt
    16921 actgaaactg tgcggtatgc cggtattaat ggcataactt tgccccgcga cgaggtggta
    16981 atcctgacca ttaatcgtca gaacaatttc accttccagt actgtgccta tttcctcacc
    17041 ctgatgctta attctttccc cagtggttgt gcccggctgg tacgtttcaa agatcatcgc
    17101 cagcgtgcga ttcgggttac cgttatgaac cagcttcatt gacacaccct gactgcccat
    17161 ctcaattaag tcgtcctgat taatgacgac ctgcggctca tcaggttttt ccggctcgga
    17221 aaagaattcc gagagtgaca gaccatacac cttcagcagc ttttgcagcg tactgatggc
    17281 agggctgact ttatcttgtt ctatcgtact gatagcactg tgagtcagcc cggagagttc
    17341 ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc gttttcctgg
    17401 cgccagtccc tcatcactca tatttgcatt tccttaaact gtagtggtca gagtcgctgt
    17461 ttttcagcga tatggtgctg acaagcggtg ataaagccct cgaacaatat acgcgaaagc
    17521 gcgtactcgc tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg
    17581 atgacgctaa ccgcctcaac taagccatcc ggcgagcgag cttcaacacg caaccgtggg
    17641 ctaacgacct tcgccccttg cccatgcaga gagtttaccc aaaagttgct acattcaggt
    17701 aacaacgcag acagtaatcc cccctcttca acctgaactt catgcgacgg tgcatattgc
    17761 tgttccaccg gcagttcggg atcttcccga tgttccagca attcaggctg ttcgcacagc
    17821 ttgcgatgca acgaaccacc ggttgccacc accagttcct gtaaaccccg gcagatggcg
    17881 aaaatgggga tgcgcctttc gagtgcggca ttaattaacg ccatgctcag aagatcacgc
    17941 ccgggatcgg cgtcaggctc atcgccgttt tcaccatata ggtgcggctg cacattgctg
    18001 ggactaccag gaagataaat gccatcgagt ttcggcaaaa gttgttcaag taatgacggt
    18061 tccgccagcg catgtggtag cgcaattggc aagccgcctg cgtggatgat ggcattcagg
    18121 tacttttctt gcagagtctg ggtcgcatga cccttaagcc tgttcctgca cattacgaca
    18181 ccgataaccg gattgttcat tatattttcc atgctcaatc tcacaaagtg gactaaatta
    18241 tcgccattac tgcggtttaa atgaccttta tgttcaatat tttttcaatc tagcagtggt
    18301 ttttcatttt tgcaaaccca atttaacatt tgacaaacat ttagtttgca tacagattcg
    18361 aatggtggtc attatatttt acgctttgat aacgagcgga aaacaaacca aaggcgaaga
    18421 atcatggaaa ccaatatcgt tgaagtagag aactttgttc agcagtcaga agagaggcgg
    18481 ggtagcgcct ttacgcagga agtgaaacgc tacctggagc gctacccgaa tacgcaatat
    18541 gttgatgttt tacttactga tttgaatggt tgcttccgtg gaaaacgtat ccctgtttca
    18601 agcctgaaga agttggagaa agggtgttat ttcccggcct cggtgtttgc aatggatatt
    18661 ctgggcaacg tagtagaaga agcgggtctg ggtcaggaaa tgggcgagcc ggatcgtact
    18721 tgtgttcctg ttctcggttc cttaacccct tctgccgccg atccagagtt tatcggtcag
    18781 atgctcctga ccatggtcga tgaagatggc gctccctttg acgttgagcc gcggaacgtt
    18841 cttaaccgcc tgtggcagca gctacgccag cgcggattgt tcccggttgt agcggtagag
    18901 ctggagttct atttactgga tcgtcagcgc gacgctgaag ggtatctgca accgccctgc
    18961 gcgcccggca ccgatgaccg caatacacaa agccaggttt actccgttga taacctcaac
    19021 cacttcgccg atgtgctcaa tgatattgat gaactggcgc agttacagtt aattcccgcc
    19081 gacggtgcgg tcgccgaagc ctcgccgggt cagtttgaaa tcaaccttta ccataccgat
    19141 aacgtgctgg aagcctgcga tgatgcgctg gcgctaaaac gcctggtgcg tctgatggca
    19201 gaaaagcata agatgcacgc cacttttatg gcgaagccgt atgaagagca cgcgggcagc
    19261 ggaatgcata tccatatcag tatgcaaaat aaccgtggcg agaacgtgct ttctgacgcg
    19321 gaaggcgaag attcgccgct gctgaaaaag atgctcgccg ggatgattga cctgatgccg
    19381 tcatcaatgg cgttgctggc accaaacgtg aactcgtatc gccgcttcca gccgggaatg
    19441 tatgtgccga cgcaggcgtc gtggggccat aacaaccgca ccgtcgccct gcgtattccg
    19501 tgcggcgacc gtcataatca ccgcgtggaa tatcgcgtgg cgggtgccga tgccaaccca
    19561 tatctggtga tggcagcgat ttttgccggt attttgcatg gccttgataa cgagctgccg
    19621 ttgcaggaag aagtcgaagg caacgggctg gaacaggaag gcttaccctt cccgattcgc
    19681 cagagcgatg ccctgggtga gtttatcgag aatgatcacc tgcgccgcta tttaggcgaa
    19741 cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc tgttgcagtt tgagcgcctc
    19801 atcacagaaa cagaaattga gtggatgttg aaaaacgcgt aattccccat gcggggttgt
    19861 ttcatcggcc tgagcggagc caggcctgac atttgctggt aatccagaca tttcccggat
    19921 gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa cgattaagac tgtgacgagg
    19981 aaatgagatg atgccgatga ttaaatcccc gcatggcgag ggtggctgcg tatgcgcccc
    20041 tcctgctact gactggactc cgcccccttt gcttcccttg ctaaacaggt ttgatttcag
    20101 gttgacacga ccgcaaacct tattacgcag gggaggcagc aattatggct attaattcac
    20161 cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg aaaatcactg aaattgtggc
    20221 aggtggtaat gatgggtctg gcctatctca cgccgatgac cgtatttgat acttttggta
    20281 ttgtctccgg cattagcgac ggtcacgttc cggcgtccta tttgctggcg ctggcgggcg
    20341 tgctgtttac cgctatcagc tacggcaaac tggttcgcca gtttccggag gccggttcgg
    20401 cctataccta cgcgcaaaag tcgattaatc cgcacgtcgg atttatggtc ggctggtcat
    20461 cactgctgga ttatctcttt ttgccaatga tcaacgtttt gttggcgaaa atctatctct
    20521 ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt aaccttcgtc gccattttaa
    20581 ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt caataccctg tttgtactgg
    20641 tgcaaatctc catcatggtg gtgtttatct tcctggtggt tcagggactg cataaaggag
    20701 aaggcgttgg caccgtctgg tcacttcagc cgtttatcag cgagaacgcg cacctgatcc
    20761 cgattattac cggtgcgacg attgtctgtt tctcgttcct cggtttcgat gcggtgacca
    20821 cgctttcgga agagacgccg gacgccgcac gcgtgatccc gaaagccatc ttcctgacgg
    20881 cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt tatgcagctg ttctttcccg
    20941 atatcagccg ctttaaagac ccggacgccg cactgcctga aattgcgctg tacgtcggcg
    21001 gtaagctgtt ccagtcgatt ttcctttgca ccacgtttgt gaacacgtta gcgtctggcc
    21061 tggcctcaca tgccagcgtg tcacgtctgc tgtatgtgat ggggcgcgac aatgtgtttc
    21121 cggagcgcgt gtttggctat gtgcatccga aatggcggac tccggcactg aacgtcatta
    21181 tggtcgggat tgtcgcgatg tcggcgctgt tcttcgattt agttaccgcc acagcgttaa
    21241 ttaacttcgg cgcgttggta gcgtttacct tcgttaacct gtcggtgttc aaccacttct
    21301 ggcggcgcaa aggaatgaat aaaagctgga aggaacactt ccactattta ttaatgccgc
    21361 tggttggcgc gctgacggtg ggcgtgctgt ggattaacct cgaagccacc tcgctgacgc
    21421 tgggtctggt atgggcttcg ttaggcggcg catatttgtg gtatctgatc cgccgctatc
    21481 gcaaagtgcc gctgtacgaa ggtgacagaa cgccagtgag cgaaacgtaa tagtcggatg
    21541 cgcgccgcat tatcctgcct gagggtaatg cgccgcgcca ttaactataa aaagatgtat
    21601 gacccggatc acgagtgcta tcatccgccc ttatgcttag cgcattaacc ccaggggcaa
    21661 ataatgaacc acgatatccc gctaaaatat tttgatattg ccgatgagta cgcgaccgaa
    21721 tgcgcagagc ctgtcgcaga cgctgaacgc acaccgctgg cccactactt ccagttactg
    21781 ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag cccaacatga aatggctgct
    21841 gaagcgggga ttaatcccgt acgcattgat gagatcgcag agtttttgaa tcaatggggt
    21901 aatgagtaat tatttaattg cagacgatcg gtcagtatcg ttgtccggat aaagcgttca
    21961 cgccgcatcc ggctaacaat gacacgcact gcagcgtgct ttaatgggtt tctcacgatc
    22021 gtaccgctct gctcctcgct aagttgttct gtgagttata gttatagaga aatcacgggt
    22081 acgaatgttg aacagatggc gacgccacct gttcaacgac caaccacacg gccctcatta
    22141 cgacccctga acacaaacgg taatggcaaa tgtcattaaa tattagtaag atagcctctt
    22201 tacataaaat cccttaatat tatgcgccag gtattatcgt ctcttttggt gattgctgga
    22261 cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga tatccgcgac
    22321 agcggttttg tctattgcgt cagcgggcaa gtcaacacct ttaacccatc caaagcgagc
    22381 agtgggttaa ttgtcgatac ccttgccgcc cagttttatg atcgactgct ggatgtcgat
    22441 ccctatacct atcgcctgat gccggaactt gccgaaagct gggaagtact cgacaacggc
    22501 gcgacctatc gcttccacct gcgtcgcgat gttccgtttc aaaaaaccga ctggtttact
    22561 cccactcgta aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat ttttgaccgc
    22621 aacaacccgt ggcataacgt caacggcagc aacttcccct acttcgacag cctgcaattt
    22681 gccgataacg tgaaaagcgt ccgcaaactg gataatcata ccgttgagtt ccgactcgcc
    22741 cagccggatg cttctttttt gtggcacctc gcaacccatt atgcttcggt catgtcggca
    22801 gaatatgccc ggaagttaga gaaagaagat cgccaggagc aactcgaccg tcaaccggtc
    22861 ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc aatttattcg cctacaacgt
    22921 catgatgact tctggtgcgg taaaccgtta atgccgcagg tagtggtgga tttaggctcc
    22981 ggcggcaccg gacgtctgtc gaaactcctg accggggaat gcgacgttct ggcctggcct
    23041 gctgccagcc agctatccat tttgcgtgac gacccgcgct tgcgtttaac gctgcgtcct
    23101 gggatgaacg tcgcctatct ggcatttaac accgccaaac cgccgctaaa taatcccgct
    23161 gtccgccatg cactggcact ggcgattaat aaccagcgcc tgatgcaatc catctattat
    23221 ggtacggctg aaacggcggc ctctatttta ccgcgcgcct cgtgggccta tgacaacgag
    23281 gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac agttgaaggc gttggggctg
    23341 gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc aggcgtggaa ccccagtcca
    23401 ctgaaaactg ccgaactgat tcaggcggat atggcgcagg ttggcgtaaa agtggtgatt
    23461 gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg atatgagcca tgatctgacg
    23521 ttatccggtt gggcgacgga cagtaacgac ccggacagtt tcttccgtcc gttactgagc
    23581 tgcgcggcaa ttcattcaca gaccaacctc gcccactggt gcgatccgaa attcgacagc
    23641 gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc gtattgaagc ctatgacgaa
    23701 gcgcagagta ttctggcgca ggaattacct attctgccgc tggcgtcgtc attgcgtttg
    23761 caggcctatc ggtacgatat caaaggtctg gtacttagcc cgtttggtaa cgcctccttt
    23821 gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac catgattatc ttcaccttac
    23881 gccgcatttt gttattgatt gtcaccttgt tcctgctgac cttcgttggc ttcagtttga
    23941 gctatttcac tccacacgcg ccgttacaag gcgcgtcact gtggaatgcc tgggtgttct
    24001 ggtttaacgg cctgatccac tgggattttg gtgtttccag cattaacggt caaccgattg
    24061 ccgaacagtt aaaagaggtc ttccctgcga cgatggaact gtgcatcctc gccttcggtt
    24121 ttgcgctgat tgttggcatc cccgtgggga tgattgccgg gattacgcgc cataagtggc
    24181 aggataactt gatcaacgcc atcgccttgc tgggcttttc aatcccggtg ttctggctgg
    24241 cgcttctgtt gacgctgttt tgttcactca cgctaggttg gttgcccgtt tcagggcgtt
    24301 tcgatctgct gtacgaagtg aaaccgatta ccgggtttgc gttgattgat gcctggcttt
    24361 cggactcacc atggcgggat gaaatgatca tgagcgcaat ccgccatatg atattgcccg
    24421 tgatcaccct gtcggttgcg ccaacaactg aagtgatccg actgatgcgt atcagcacta
    24481 tcgaagtgta tgaccagaac tatgttaaag cggcggctac ccgcggtttg tcgcgcttta
    24541 ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc ggttattcct cgcctgggct
    24601 tacagttttc caccatgctg acgctggcga tgatcaccga aatggttttt agctggccgg
    24661 gtttagggcg ctggttaatt aacgccatcc gccagcagga ttacgcagcc atttccgccg
    24721 gagtgatggt gtgtggctca ctggtcatta ttgttaacgt gatttctgat attttgggtg
    24781 ccatggctaa ccctctgaaa cataaggaat ggtatgcctt acgatagcgt atacagcgaa
    24841 aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga cgcctctgcg
    24901 atggtcggcc tgtacggctg cgcggggctg gctgtactgt gtatttttgg cggctggttt
    24961 gcgccttacg gcatcgacca gcaatttctc ggttaccaat tattgccgcc gtcatggtcg
    25021 cgctatggcg aagtttcttt cttcctgggg actgacgacc tcggacgcga tgttttaagc
    25081 cgtttactga gcggagctgc gccaaccgtg ggtggcgcat ttgtggtgac gcttgccgcg
    25141 acgatctgtg gcctggtact gggcaccttc gccggggcga cgcacggcct acgctcagcg
    25201 gtgcttaacc atattctgga taccttactg gcgatccctt cgctgctgct ggcaattatc
    25261 gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt ttgctgtctg gctggcattg
    25321 ctgccgcgta tggtacgttc gatttacagc atggtgcatg acgaactgga aaaagagtac
    25381 gttatcgccg cccgtctgga tggcgcatca acgctgaata ttctctggtt tgctgtgatg
    25441 ccaaacatca ccgccgggct ggtcaccgag atcacccgcg cactgtcgat ggcaattctc
    25501 gatatcgccg cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc tgaatgggga
    25561 gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt catgctgcca
    25621 ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc ttggcgatgg cgttcgtcgt
    25681 gcgattattg cgggggtgga ataatgccat tactcgatat tcgtaacctg accattgaat
    25741 ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt aagtatgacc ttaaccgaag
    25801 gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa gagtttgatt gcgaaagcga
    25861 tttgtggggt gaataaagat aactggcgtg ttactgctga ccgtatgcgt tttgatgata
    25921 tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact ggttggccat aacgtgtcga
    25981 tgattttcca ggaaccgcag tcgtgtcttg acccttcaga acgtgtgggt cgccagttga
    26041 tgcaaaacat cccagcctgg acctataaag gccgttggtg gcagcgtttt ggctggcgca
    26101 aacgccgtgc cattgaactg ctgcaccgcg tggggattaa agatcacaaa gatgcgatgc
    26161 gcagttttcc ctatgagttg accgaaggtg aatgtcagaa agtgatgata gccattgcac
    26221 tggcgaatca accgcgtctg ctgattgctg acgaaccgac caactcaatg gagccaacaa
    26281 cccaggcgca aatctttcgc ctgctgacgc gtctcaacca aaacagcaat accactattt
    26341 tgcttatcag ccatgactta caaatgctta gccaatgggc ggataaaatt aacgtgcttt
    26401 actgcggtca aacagtggaa accgcgccga gtaaggagtt ggtgacgatg ccacatcatc
    26461 cttataccca ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg ccgcataaaa
    26521 gtcgcctcaa tacgctgccc ggcgctatcc cactgctgga acagttaccg attggttgtc
    26581 gtctggggcc acgttgcccg tatgcacaac gagaatgcat tgtgacgcca cgtttgacgg
    26641 gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa catggagaaa gagtgagatg
    26701 atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg gaccggctgg
    26761 tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct ttacgctacg tgaaggccag
    26821 acactggcga ttattggcga gaatggttcg ggtaaatcca cgctggcaaa aatgctggcg
    26881 ggaatgatag agcctaccag cggcgagtta ttgattgacg atcatccact gcattttggc
    26941 gattattcct tccgcagtca gcgtattcgc atgatttttc aggatccttc gacctcgttg
    27001 aatccccgtc aacgtatctc gcaaattctg gattttccac tgcgcctgaa caccgacctg
    27061 gaaccggaac agcgtcgcaa acagattatt gaaacgatgc gtatggttgg gctgctaccg
    27121 gatcacgtca gttactatcc gcatatgttg gcacccggac aaaaacagcg tctgggtctg
    27181 gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg atgaagccct cgcttcactg
    27241 gatatgtcga tgcgttcgca gttgattaat ctgatgctgg agttacagga aaaacagggc
    27301 atttcgtata tttatgttac ccagcatatc ggaatgatga agcacattag cgaccaggtg
    27361 ctggtgatgc atcagggcga ggttgtcgag cgaggcagca ccgcggatgt gctggcatca
    27421 ccgctgcatg aactcaccaa acggctgatt gccgggcact ttggcgaagc attgacagcg
    27481 gatgcgtggc gtaaagatcg ctaattttct gaatcctcct tccagattat tcacctcatt
    27541 ctgacaagag attaaatctt gtcagaatga ggtgatggat aaaataaaat caaatgcgcg
    27601 tgatttaaga agcaatctca ctttgcagga acgaaagctc tggcgatatc ttcgcagccg
    27661 acgttttggt gatttcaaat ttcgccgtca acatccggtg gggagctaca ttctcgattt
    27721 tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt gggcagcatg atttggcagt
    27781 tgcctatgat accaggcgca ctagctggct tgagtcgcag ggctggaccg tgctgcgttt
    27841 ctggcataac gagattgatt gtaatgagga ggcggtgctg gagattattc tgcaggaact
    27901 gaaccgccga tcaccctcac cctgaaaggg cgagggggcc gaccgagccg aatagctgtt
    27961 gtggtgaaaa catggagaca gtgctggaga atattcggca aggtctgaac catcccagcc
    28021 atcgccataa aagggttagg ggctgtatga gcctgtttgt tgctggggta acaatatttg
    28081 cacaatatgg tcccctcgcc cctctgggga gagggttagg gtaaggggaa aggcgcccac
    28141 cctgccgcag cctgctccgg tcggacctgg caactatagc tactcacagc caggttgatt
    28201 ataataaccg tttatctgtt cgtactgttt actaaaacga cgaatcgcct gattttcagg
    28261 cacaacaagc atcaacaata aggattaaag ctatgggttt tctttccggt aagcgcattc
    28321 tggtaaccgg cgttgccagc aaactatcca tcgcctacgg tatcgctcag gcgatgcacc
    28381 gcgaaggagc tgaactggca ttcacctacc agaacgacaa actgaaaggc cgcgtagaag
    28441 aatttgccgc tcaattgggt tctgacatcg ttctgcagtg cgatgttgca gaagatgcca
    28501 gcatcgacac catgttcgct gaactgggga aagtttggcc gaaatttgac ggtttcgtac
    28561 actctattgg ttttgcacct ggcgatcagc tggatggtga ctatgttaac gccgttaccc
    28621 gtgaaggctt caaaattgcc cacgacatca gctcctacag cttcgttgca atggcaaaag
    28681 cttgccgctc catgctgaat ccgggttctg ccctgctgac cctttcctac cttggcgctg
    28741 agcgcgctat cccgaactac aacgttatgg gtctggcaaa agcgtctctg gaagcgaacg
    28801 tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg tgttaacgcc atctctgctg
    28861 gtccgatccg tactctggcg gcctccggta tcaaagactt ccgcaaaatg ctggctcatt
    28921 gcgaagccgt taccccgatt cgccgtaccg ttactattga agatgtgggt aactccgcgg
    28981 cattcctgtg ctccgatctc tctgccggta tctccggtga agtggtccac gttgacggcg
    29041 gtttcagcat cgctgcaatg aacgaactcg aattgaaata atcattctgt tggtaaatat
    29101 tgggcggcgt tctgccgccc gttatctctg ttataccttt ctgatatttg ttatcgccga
    29161 tccgtctttc tccccttccc gccttgcgtc aggataacga tttcctttac gaccaaggag
    29221 cgcccatgga acaacgccac atcaccggca aaagccactg gtatcatgaa acgcaatcca
    29281 gtactgcgga gtatgacgtt ctgcctctgg tcccggaagc cgcaaaggtc agcgatccct
    29341 ttctgctcga cgtgatcctt gatgaagaaa cgctggctcc cttcctttca tggctggtcc
    29401 ctgcgcgcgt tcttgcagtg gaattgttcc ctgaccagct taccgtaacc cgttcacaga
    29461 ccttcaccgc ttatgaacgc ttgtctacgg ccctgacggt tgctcaggtt tgcggcgtcc
    29521 agcggttatg taactactat tcggcgcgac ttacgccgct ccccgggcct gattcctcca
    29581 gggaaagcaa tcataggttg gcacaaatca cgcaatatgc ccgccaactg gctagctcgc
    29641 cttctattat cgacaaccga tcgcgccagc atctgaatga cgtcggtctt actgccgggg
    29701 actgtgtgat cattaaccaa atcattggtt ttattggctt tcaggcgcgg accattgcga
    29761 catttcaggc ttatctcggg cacccggtac gctggttacc cggtctggaa atacaaaact
    29821 acgccgacgc gtcactgttt gctgatgaat caatacgctg gcgaagcagt tatgaggtgg
    29881 aaaaactacc tgaagagtac acaaaaagtt caacagcaga actttgccaa ctggccgaaa
    29941 cactctctct ccaccctatt tcactttccc ttctcgaaag gttgttaaac agtacacggg
    30001 ttaatacaca gccggataat aagcttgcgg cgttgttatg cgcgcggata aatggcagtc
    30061 ctgcttgttt tgccgcctgt atggattcat caaatgaata taaaaaaatc agcccccttc
    30121 tgcgcaaggg cgaaaatgaa attaaccaat gggctgaccg tcattctgtt gagcacgcta
    30181 ccgttcaggc gatacaatgg ctgacccgag cacccgatcg ctttagcgcc gcccagttca
    30241 gccctttact cgaacacgaa aaatcatcaa cgcagattat taatctgctg gtatggagcg
    30301 ggctgtgtgg ctggataaat cgcttaaaaa tcgcgttggg tgagacatat taaccttgcc
    30361 gcgtcagaca gattcgcgta aaactgtcag ccgctctaat ggccaccaaa atagacaatt
    30421 atgtttcagg acaacccgct gctagcgcag cttaaacagc aactgcattc ccagacgcca
    30481 cgcgctgaag gggtggtaaa agccacagaa aaaggctttg gcttcctgga agtcgacgcg
    30541 caaaaaagtt atttcattcc gccgccgcag atgaaaaaag tcatgcatgg cgaccgaatt
    30601 atcgcggtga tccacagtga aaaagaacgt gaatccgcag agccagaaga actggttgaa
    30661 ccgttcctga ctcgtttcgt gggtaaggtt cagggcaaaa atgaccgtct ggccatcgtt
    30721 cctgatcatc ctctcttaaa agacgccatt ccttgccgcg cagcccgtgg cctgaaccac
    30781 gagtttaaag aaggcgactg ggcggttgcc gaaatgcgcc gtcatccgct gaaaggcgat
    30841 cgttctttct atgcagaact gacacaatac atcacttttg gtgacgacca ctttgtaccg
    30901 tggtgggtta cccttgcacg ccataatctg gaaaaagaag caccagacgg cgtcgctacc
    30961 gaaatgctcg atgaaggtct ggttcgtgaa gatctgaccg cgctggattt tgtcaccatc
    31021 gacagtgcca gcacagaaga tatggatgac gcccttttcg ctaaggcgtt gccggatgac
    31081 aaacttcagc tgattgtggc gattgccgat ccaaccgcgt ggattgctga aggcagtaag
    31141 ctggacaaag ccgcgaaaat tcgcgcattc accaactatc tgcctggctt caacatccct
    31201 atgctgcctc gcgagctttc tgacgatctc tgctcactgc gcgccaatga agtccgcccg
    31261 gtactggcat gccgcatgac gctctccgct gatggcacca ttgaagataa tatcgaattc
    31321 tttgccgcca ccatcgaatc caaagcgaag ctggtgtatg accaggtttc tgactggctg
    31381 gaaaataccg gtgactggaa gcctgaaagt gaagcaattg ccgaacaagt ccgtttgcta
    31441 gcgcaaattt gccaacgccg cggcgagtgg cgtcataacc acgcattggt gtttaaagat
    31501 cgcccggatt accgctttat tctcggtgaa aaaggtgaag tgctggatat cgtcgccgag
    31561 cctcgtcgca ttgccaaccg tatcgtcgaa gaagcgatga ttgccgctaa catttgtgcg
    31621 gcccgcgtac tgcgcgataa gctcggtttt ggcatctata acgtgcatat gggctttgat
    31681 ccggcgaatg ccgacgcgct ggcagcgttg ctgaaaacgc acggtctgca tgtcgatgcc
    31741 gaagaagtgc tcacgctgga cggtttctgc aaactgcgtc gtgaactgga tgcgcaacca
    31801 actggtttcc tcgacagccg cattcgtcgc ttccagtcat ttgctgaaat tagcactgaa
    31861 cccggtcctc actttggcct cggtctggaa gcatacgcca cctggacttc gccgatccgt
    31921 aaatatggcg acatgatcaa ccaccgtctg ctgaaagcgg ttatcaaagg cgaaactgcg
    31981 acgcgcccac aggatgaaat cactgtccaa atggccgagc gtcgccgtct caaccggatg
    32041 gcagaacgtg atgttggcga ctggttatac gcacgcttcc tgaaagacaa agccgggacc
    32101 gacacccgtt tcgcggcgga aattgtcgat atcagccgtg gcggcatgcg tgtgcgtttg
    32161 gttgataacg gcgctatcgc ctttattccg gcacctttct tacacgctat gcgcgatgaa
    32221 ctggtttgca gccaggaaaa cggcactgta caaattaaag gtgaaacggt ttacaaagta
    32281 actgacgtta ttgacgtcac cattgccgaa gtccgcatgg aaacccgcag cattattgcg
    32341 cgcccggtcg cgtaatctcc tttcacggcc cattcctcat ggatgggccg tttatttccc
    32401 cgctctacct tcgttatatc caggcagatt ttttaactga ctttcgtttg aaaactggcg
    32461 tttttccaaa actggattac aaataaatat ataaaaaaaa caatgcgttt agccgccgtt
    32521 aatttcatgt aagtcatgtt gttcctttta tttaaccgcg gactccgctg ttaaccggag
    32581 gatatgcatc atgaaaaccg ttagggagtc cacaacgttg tacaactttc tcggatcgca
    32641 caatccatac tggcggttga cggaaagcag cgatgttttg cgcttttcta ccaccgaaac
    32701 cacagaacct gatcgtacat tgcagttatc tgccgaacag gctgctcgca tcagggaaat
    32761 gacggtcatc acctccagcc tgatgatgag tctgaccgtc gatgaaagcg atctttctgt
    32821 gcatctggta ggacgaaaaa tcaataaacg ggaatgggct ggcaacgcgt ctgcatggca
    32881 tgacacaccg gcggttgctc gtgatttatc acacgggctt tcctttgctg agcaggtagt
    32941 ttctgaagca cattccgcaa tagtgattct cgacagccgg gggaatatcc aacgcttcaa
    33001 tcggttatgt gaagattaca cagggttgaa agaacacgac gtcattgggc aaagcgtgtt
    33061 taaactgttt atgagccgtc gtgaagctgc ggcatccagg cgcaataacc gtgtattttt
    33121 tcgaagcggc aatgcatatg aagtcgaact gtggatacca acatgtaaag gccagcggct
    33181 gtttctgttt cgcaataaat ttgtccacag cggcagtggc aaaaacgaga tttttttaat
    33241 ctgttccggc accgacatta ccgaagagcg ccgcgctcag gagcgactgc gtattctggc
    33301 aaataccgac agtatcaccg gactgccgaa tcgtaacgca atgcaggatt taatcgatca
    33361 cgctattaat catgcagata acaataaagt tggggttgtg tatcttgatt tggataattt
    33421 caaaaaggtc aacgacgcct atgggcattt gtttggtgac cagttattac gcgacgtgtc
    33481 attggctatt ttaagctgtc tcgaacatga ccaggtgttg gcgcgtccag gtggggatga
    33541 gtttctggta ctggcatcca acacctcaca aagcgcgctg gaagcaatgg catcacgaat
    33601 tttgacccgc ttacggctcc cctttcgcat tggtttaatt gaagtttata ccagctgttc
    33661 agtaggtatt gcactctctc ccgaacatgg ttcagacagc acggctatta ttcgtcacgc
    33721 cgacacagca atgtacacag cgaaggaagg cggacgagga caattttgcg tttttacccc
    33781 agaaatgaat caacgggtat ttgaatatct ctggctggat accaacttgc gtaaagcact
    33841 ggaaaacgat cagttggtta ttcactatca accgaaaatc acctggcgtg gcgaagtgcg
    33901 cagtctggaa gcactagtac gttggcagtc acctgaacgt gggttgattc caccgttgga
    33961 cttcatttcc tacgccgaag agtcagggct aattgtgcct ttaggccgtt gggtgattct
    34021 cgatgtcgta cgccaggtgg caaagtggcg ggataaaggc ataaacctgc gagtggcggt
    34081 aaatatttct gcacgtcagc tcgccgatca aaccattttc accgccctga aacaggttct
    34141 ccaggaactc aattttgaat actgccctat agatgttgaa ctgacagaga gttgtctgat
    34201 tgagaatgat gaactggcac tgtctgttat tcaacaattt agccaactag gtgcgcaagt
    34261 gcatctggat gattttggta ccggctactc ttcactttcg caactggcgc gctttccgat
    34321 cgatgccatc aaacttgacc aggtttttgt tcgagatatt cacaaacaac ctgtctcgca
    34381 gtcactggtc cgggcgatcg tcgctgtggc ccaggcattg aatcttcagg tgatcgccga
    34441 aggtgtagag agtgcaaagg aagatgcttt tttaaccaag aacgggatca atgagcggca
    34501 aggatttttg tttgccaaac cgatgcccgc cgtcgccttc gaacgctggt ataaacgcta
    34561 tctgaagcgc gcataagtag atgaatatat atacttagcg ctatatcccg tcattatata
    34621 tcgtattcct ggatgataat tagccgttgc tgttaaacac ttctctttta gcggcaattt
    34681 ttgcattatt cattttccac ttttgctata aggatgtcct tgatgtctga atttgatgcc
    34741 cagcgcgttg ccgaacgtat tgatattgtg ttagatatcc ttgtggcagg cgattaccac
    34801 tctgcgatcc ataatctcga gatacttaaa gcagaactct tgcgccaggt tgctgaatct
    34861 acgccagaca tcccgaaggc accgtgggag atttaacaat cgtgccttca ggttttccta
    34921 tttgaggatg cccggcgcgt cagtattttt ttatttagta ttataacgtt ataagaatta
    34981 cagcgatgaa ttcccgacaa caaactattc tacagatggt cattgaccag ggtcaggtta
    35041 gcgtaaccga tctggcaaaa gccactggag tttctgaagt taccattcgc caggatctca
    35101 acaccctcga aaaactgagt tacctccgcc gtgcacatgg ctttgcagtt tcgcttgata
    35161 gtgatgacgt cgaaacccgt atgatgagca actatacgct gaagcgtgaa ctcgccgagt
    35221 ttgccgcgtc tctggttcaa ccgggcgaaa ccatctttat cgaaaatggt agcagcaatg
    35281 ccctgctcgc ccggactctg ggcgaacaga agaaaaatgt cactatcatc acagtcagca
    35341 gctacattgc gcatttgctg aaagacgcgc cttgtgaagt cattttgctc ggtggcgtgt
    35401 accagaaaaa aagcgaaagt atggttggcc ctttgacacg ccagtgcatc cagcaggtac
    35461 atttcagcaa agcatttatt ggtattgatg gctggcaacc tgaaactgga ttcaccggtc
    35521 gcgatatgat gcgtaccgat gtggtcaatg ccgtgctgga aaaagagtgc gaagcgatag
    35581 tcctgactga cagctcgaaa tttggtgctg tacattcata ctccatcggt cccgttgagc
    35641 gattcaatcg cgtgattacc gattcgaaaa tacgcgccag cgatctgatg catcttgagc
    35701 acagtaaact caccgttcac gtcgttgaca tttaaccatt ccccttctct acggatgatt
    35761 tgcagtttgg caaatcatcc gctctaagat gattcctggt tgataattaa gactatttac
    35821 ctgttattaa cacgcttaag atataaaatt attatcagcg atataacagg aagtcattat
    35881 cacctgcgtg atataaccct gcgcgcgagc agatttcacg gaataatttc accaggctta
    35941 ttcttagcta ttatagttat agagagctta cttccgtgaa tcataaattc aggagagagt
    36001 attatgtttg taacgagcaa aaaaatgacc gcggctgttc tggcaattac tttggcaatg
    36061 tctctgagtg cctgttctaa ctggtctaaa cgggaccgca acaccgcaat cggcgcgggt
    36121 gcaggggcat taggcggtgc agtactgacc gatggcagta cgttgggtac attaggtggt
    36181 gcagctgtcg gtggtgttat tggtcaccag gtcggtaaat aatcgtaaat aaatgtcgat
    36241 tatcgactcg caattataat acgcttgatt ctgttcagta cgaaataact gaataatacg
    36301 cacagtaaaa tattcgaaag ccacggatat atccgtggct ttttatgtta accgcctgcg
    36361 agttttacct tcatcccttt cgcttcaagt agtgatttta ataaatcacg cttatcgccc
    36421 tggatttcaa taactccatc tttaactgct ccgccgcagc cgcatttttt cttcagttcc
    36481 gctgcaagtt ttgtcagttc ggcatcatcg agatcgacac cggtaatcag gcaaacgccc
    36541 ttacctttac gtccactggt ctgacgctga atacgcacca caccgtcgcc tttagggcgt
    36601 acaggggccg ctttgggctc atcaatacgt ccggtttccg ttgagtagac cagacggctg
    36661 ttggaatcac tcatcatgca ctccgctgta aagaggcgtt gatcgctttc agcgtctgcg
    36721 ctggatctac cgattgcgtt accgggcgac caatcaccat ataatcaaca ccagccgcca
    36781 acgcctgttc tggcgtcata atgcggcgct ggtcaccagc atcactcccc tgcggacgaa
    36841 tgcccggtgt aaccagtttg aactcctgac cgaatacctg tttaaagcgc acagcttcct
    36901 gagcagaaca caccacacca tcaaggccac atttttgcgt cagtgccgcc agacgttctg
    36961 cataatctgc aggtgacagt gtcacgccaa gatcggccag gtcgctagct tccatgctgg
    37021 tcaacactgt cacagcaatc aaaagcggtg catctttgcc aaacggaacc agtgcctcac
    37081 gcgctgcggt catcatacgc gccccaccag aggcatgaac attcaccatc cacacgccta
    37141 agtcagctgc agcagcgaca gcgtgcgctg cagtgttagg aatatcgtgg aatttcaggt
    37201 caagaaagat atcaaaacca cgctgttgaa gttcgcgcac aaactgtggc ccaaacaatg
    37261 taaacatctc tttgccgacc ttcagacgac aatcgcgtgg gtcgatcttg tcgacaaagg
    37321 acagcgcgtc atcacgatta tgataatcaa gggcaacaac cacaggagaa ttcgtaacag
    37381 cgcgggaaga agatgaagca gttaacgtca tgaccagacc ttcttgatga tgggcaccgg
    37441 aagtggcgcg aaacaggtaa acggcgagca ttctacccgt ggaaataaaa aattaacagt
    37501 tgcgattttt cccctggcag gctttttctg ctccgtcgtt gccggagcac aatggaacat
    37561 caataactag tatgttgtaa ctaaagtcgg atttttttaa aaattacagg ccatcaagac
    37621 cgcgaatcgg tttaatggtt gaccaggccc gacaagacgg acaatgccag tagagggtgt
    37681 atgcggtaaa accacatttc tggcagcgat aacgaggctt actacgtacc ttttcgccaa
    37741 ccatgtcacg cagcaccatc aggctctctt tggcacgccc ttcttccgct tcatttaagt
    37801 ggtaatccat taacttatgg aacacacgca tggtcggatg acgctgaagc tggcgcgtaa
    37861 tatagacctg tgcggcctca ctaccgtcgc gcgcttcgat gatatcagca agcatcaatt
    37921 cagcatcggc accggtgttc tcttccaccg cgcgttgcag gaattctgcc cattcggcag
    37981 ttttacccaa ctgctgatag caggtttgca acatttccag cgtttcgctg accagttctc
    38041 tgtcctggga tatcacgcgt tgcaaacttt cgacggcttt ggcgtattct cctttcgcca
    38101 taaacacgcg tcccatcatg atggatacgc gggcgctgtt tttatctgcc gccgcccctt
    38161 tttttagcaa ggtcatggca cgatcgagat cgtcgctggc catatgctgc agggctaact
    38221 cacagtagaa atgggcaatt tcgacgcgct gtttatcttt acccagcttc accaggcgtt
    38281 cggcaacatc aattgctttc tgccactcgc tggtagcctg gtagatttgt agcaactgtt
    38341 gcagcgcgcc aatgcggaag tcagtttcat cggtcagctg attgaacatg tcttccgcgc
    38401 ggtcgtataa cccggcggcc atgtaatcac gccccagttg ttgaatcgcc aacagacgct
    38461 gttcataggt cagtgaggcg ctttccatta gggtctgatg gatgcgaata gcgcgatcaa
    38521 cttcgccacg cgaacggaac aggtttccga gcgtaaggtg ggcttcaacg gtgcctgtat
    38581 cctctttaag catatcgaga aacaggtcta ccgctttatc ctgttgatta ctaagcagga
    38641 agttaacccc cgctacgtaa tcacgcgaca agcggttggc ttcatcttgc ttgttttgtt
    38701 gcgcacttct gcggcccata taccagccat aggcagcggc tacaggcaaa agcagaaaca
    38761 acaactccag catagaaagt tattccttcg ccgctgacga gtgcggcact acagccacgt
    38821 cagtcgcggg tgaaagctgg ttttccagtc gctttatttt acgttcagcg cgcgccaggg
    38881 aaacacgaac tcgcagccag aacaggccac aaatcaacca accgatagca aaccccgcag
    38941 caaacaatac cgccaacaat gtggaaatac ggtactcccc ttgcgctaac agataattaa
    39001 acgtcacctg ttgatcgttc tgcgcaccca acgtgaccga aatcacgaag atcgctaaca
    39061 ccagtaagaa aatgagtaaa tatttcacat tacttcccgt tatgtggtta gagcgaataa
    39121 attatggtca acttaccgca atagcccttt aaagtaccat tttcgcgccg tctgcgaaat
    39181 ggcatccacg cgcattgttg atgatttaag ggctaaattc ggaaaatcaa ccagcgttaa
    39241 ctttcttgtt ctcgttgcgc tatttcgcga ttttcttccg caggtggtgt taatggccca
    39301 caaattcgtt gcgcaagcca cgttgccacc gccaccagcg cccacgaaat caacgtagct
    39361 actaccagat cgcgtggcca atgcatcccg agcagcaggc ggcttcccat gactcccgtt
    39421 gcccagacca gcaagatagc aatggttaac gttcgccgac gcggccacag caaaccaacg
    39481 gccagcagtg cccaactggc agcaaacatc gtgtgaccgg aaggaaaggc aaaccccgtc
    39541 tctttctgcc agtgtgaacg caaatattgt gggatatttt tctcttcagc caactgttct
    39601 ttcactaaat ttccgcgttc tgctcgcttt aaagtgtaga actcatcaac cggaatatga
    39661 tgtgtttttt ccagccagat aacaaaaggt cgtggttcct ggactttgtc tttgatccag
    39721 gatttaacgc cttgtcccac aaggattgcg gccgccagaa tggcaaataa tacaaaggca
    39781 gccttaatgc gaaaacgcag acaccagaga aaccagccga ataaaatcaa atgtgtaatg
    39841 acgccccagg gctgggtgac agtttcagta acccaaaaag ccgcttttag tagccaactt
    39901 tgttctccag gttgccaacg ccagccagaa atccatacgg ctactggcat gacaagcaat
    39961 agtgcagctc ccactgcggt acgtctggca atcgaacgca tggcctctcc tttttgataa
    40021 gtcccacaat cataactgaa aacgccagtt ccaggaaaaa ttgacagatt tgtgccattc
    40081 cgtgaacgat cgacgcgtcg tgattaggtg aaccccttct cgttatggca aaataagcca
    40141 atacagaacc agcattatct ggagaatttc atgcagctta aacgtgtggc agaagccaaa
    40201 ctgccaaccc catggggcga tttcctgatg gtgggatttg aagaactggc aaccggacac
    40261 gatcatgtcg cgctagtcta tggcgatatt tccgggcata ccccggtact tgcgcgcgtc
    40321 cattccgaat gtctgaccgg tgacgccctg ttcagcttgc gctgcgattg tggcttccag
    40381 ctcgaagcgg cattgacgca aattgccgag gaaggccgtg gtattttgct gtatcaccgt
    40441 caggaaggtc gtaacattgg tctgctgaat aaaatccgcg cttacgcact gcaggatcaa
    40501 ggttacgata ccgtagaggc taaccaccag ttaggcttcg ccgctgatga gcgcgacttc
    40561 actctttgcg ctgatatgtt caaactcctt ggcgtcaatg aagtccgctt gttaaccaat
    40621 aacccgaaaa aagtcgaaat tctgaccgaa gcagggatta atattgttga acgcgtacca
    40681 ttgattgtag gtcgtaaccc caataacgaa cattatctcg ataccaaagc cgagaaaatg
    40741 ggccatttgc tgaacaaata accctcttgc attgtgtaat tagtttgctt gccggaagca
    40801 aaataaccgg caagcaaata cttgttactt caacatatta cgaatgacat aatgcaaaat
    40861 gccgtcgttc tggtagtagg tcaactccgt cgcggtgtcg atacgacaac ggcagggtac
    40921 gacctcctgg ctaccatccg cgcgcgtaag cgtcaccgga accgtcgcac cgggttgtag
    40981 attttgcaga tcgccaatat caatcttctc ttccccggtt agtcctaacg ttttacgcgt
    41041 tacgccttgc ggaaattcca gcggcaggat gcccatgcca attaaattcg aacggtgaat
    41101 tcgttcaaac gattcggcaa tcaccacacg aattccaagc agacgaggac cttttgccgc
    41161 ccagtcacgg cttgagcctg atccatactc tttcccggca atcaccgcca gcggcgtttg
    41221 ttcctgctta tagcgcatcg cagcatcata aatagagact acgtcgctgt caggtaaatg
    41281 ccgcgtcatc cccccttcaa cgccaggcac catttcatta cggatgcgaa tattggcgaa
    41341 ggtgccgcgc atcatcactt catggttacc acgccgcgaa ccgtaggagt taaagtcttt
    41401 tcgctcaaca ccccgacctt gtagatatcg acccgctggg ctgtcgggct taatactgcc
    41461 cgccggagag atatggtcag tggtgactga atcccccagc attgcgagga tccgcgcacc
    41521 gtgaatatct tccactggtg ctggtgttgc ctgcatttca tcaaagaaag gcgataagcg
    41581 aatataggtt gagtcctcct gccaaccgta ggtatcggat cgtgtgacgt taatttcctt
    41641 ccactctgct gtgccttcaa aaacttctgc gtactctttg cggaacattt ctgtggagac
    41701 ttgttctacc gcacgggcaa tttcttgtgc cgatggccag atatctttca gatataccgg
    41761 atcgccttta cgatcatggc cgataggctc agaagccagg ttgatattca tatttcccgc
    41821 cagcgcatag gcaaccacca gcggcggcga ggccagccag ttggttttaa ccagcggatg
    41881 gatacggcct tcaaagttac ggttgccgga cagcactgca ccgacggtta aatcgccttt
    41941 tttgattgcc gtttcgatag gatcgggcag cggcccagag ttaccaatac aggtggtaca
    42001 accgtatccc acaaggttaa accccagttc gtcgagatac ggtgtcaatt tcgcttttgc
    42061 cagataatca gaaacaactt tcgagcctgg tgccagcgac gctttgaccc acggttgccg
    42121 cttgaggcca agagttacgg cttttttcgc cagcaagcct gcagccatca gcacacttgg
    42181 gttagaggtg ttggtgcacg aggttatcgc agcaatgacc acagcgccat caggtaactg
    42241 atactgatgt ccgttcataa cataatcgac cggctggcga tctttatgcg tggcattcac
    42301 ttccagttcg ttactggcgg caaatgcttt tggtacatcg ggcagtgcaa cgcgatcctg
    42361 tgggcgtttt ggccctgcca ggctcgcttc aacgtcattc atatccagtt ctaacgtact
    42421 ggtaaaaatc ggttcatcgc ccgggttacg ccacatgccc tgcgctttgg catatttttc
    42481 gaccaactca acctgatctt cgctgcgccc gcttaaacgc atgtaatcga gggttacagc
    42541 atcgattggg aagaagccac aggtggcacc atattctggc gacatattgg caatggtggc
    42601 gcgatccgcc agcggtagtg aatccagacc atcaccataa aattcgacga atttccccac
    42661 cacgccatgt ttgcgcagca tttgggtaac agtgagaacc aggtctgtgg cggtaatacc
    42721 ttcacgtaat ttaccggtaa gtttaaagcc cactacgtcc gggataagca tggaaaccgg
    42781 ct
//
LOCUS       DAZGAN020000030        41773 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGAN020000030 DAZGAN020000000
VERSION     DAZGAN020000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 41773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.030"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1232
                     /locus_tag="ACHKYO_RS09250"
                     /old_locus_tag="pgaptmp_001850"
     CDS             <1..1232
                     /locus_tag="ACHKYO_RS09250"
                     /old_locus_tag="pgaptmp_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4428707.1"
                     /translation="VYLLNNMTVNDDANDVAVTESYKPVWDWKISQFNVSDIKFETMI
                     NPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATGHLRIMAEGENFLSQ
                     LLNIEDEVLNILLRENFNEQLDTNVNTIISILNRRDIVLESLQPYLVINKDAVTPCTF
                     LGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLD
                     EDTYAGIKDYPVCFYDPKYKIMANHHGITFDEQRKRYIIGPITVSIDEMTNALDPVEL
                     AAIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVV
                     AHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEK
                     NRNGLMPLIRRRTMLQL"
     gene            1422..1535
                     /gene="yhiY"
                     /locus_tag="ACHKYO_RS09255"
                     /old_locus_tag="pgaptmp_001851"
     CDS             1422..1535
                     /gene="yhiY"
                     /locus_tag="ACHKYO_RS09255"
                     /old_locus_tag="pgaptmp_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HGU4428708.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1598..2665
                     /gene="yhiI"
                     /locus_tag="ACHKYO_RS09260"
                     /old_locus_tag="pgaptmp_001852"
     CDS             1598..2665
                     /gene="yhiI"
                     /locus_tag="ACHKYO_RS09260"
                     /old_locus_tag="pgaptmp_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4428709.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAHRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2662..5397
                     /gene="rbbA"
                     /locus_tag="ACHKYO_RS09265"
                     /old_locus_tag="pgaptmp_001853"
     CDS             2662..5397
                     /gene="rbbA"
                     /locus_tag="ACHKYO_RS09265"
                     /old_locus_tag="pgaptmp_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HGU4428710.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5397..6521
                     /gene="yhhJ"
                     /locus_tag="ACHKYO_RS09270"
                     /old_locus_tag="pgaptmp_001854"
     CDS             5397..6521
                     /gene="yhhJ"
                     /locus_tag="ACHKYO_RS09270"
                     /old_locus_tag="pgaptmp_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4428711.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6625..6855
                     /locus_tag="ACHKYO_RS09275"
                     /old_locus_tag="pgaptmp_001855"
     CDS             6625..6855
                     /locus_tag="ACHKYO_RS09275"
                     /old_locus_tag="pgaptmp_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGU4428712.1"
                     /translation="MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMS
                     VACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6852..7211
                     /locus_tag="ACHKYO_RS09280"
                     /old_locus_tag="pgaptmp_001856"
     CDS             6852..7211
                     /locus_tag="ACHKYO_RS09280"
                     /old_locus_tag="pgaptmp_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGU4428713.1"
                     /translation="MIKLKTPNFMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7331..7732)
                     /gene="nikR"
                     /locus_tag="ACHKYO_RS09285"
                     /old_locus_tag="pgaptmp_001857"
     CDS             complement(7331..7732)
                     /gene="nikR"
                     /locus_tag="ACHKYO_RS09285"
                     /old_locus_tag="pgaptmp_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HGU4428714.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7738..8544)
                     /gene="nikE"
                     /locus_tag="ACHKYO_RS09290"
                     /old_locus_tag="pgaptmp_001858"
     CDS             complement(7738..8544)
                     /gene="nikE"
                     /locus_tag="ACHKYO_RS09290"
                     /old_locus_tag="pgaptmp_001858"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HGU4428715.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(8541..9305)
                     /gene="nikD"
                     /locus_tag="ACHKYO_RS09295"
                     /old_locus_tag="pgaptmp_001859"
     CDS             complement(8541..9305)
                     /gene="nikD"
                     /locus_tag="ACHKYO_RS09295"
                     /old_locus_tag="pgaptmp_001859"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HGU4428716.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(9305..10138)
                     /gene="nikC"
                     /locus_tag="ACHKYO_RS09300"
                     /old_locus_tag="pgaptmp_001860"
     CDS             complement(9305..10138)
                     /gene="nikC"
                     /locus_tag="ACHKYO_RS09300"
                     /old_locus_tag="pgaptmp_001860"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HGU4428717.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(10135..11079)
                     /gene="nikB"
                     /locus_tag="ACHKYO_RS09305"
                     /old_locus_tag="pgaptmp_001861"
     CDS             complement(10135..11079)
                     /gene="nikB"
                     /locus_tag="ACHKYO_RS09305"
                     /old_locus_tag="pgaptmp_001861"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HGU4428718.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(11079..12653)
                     /gene="nikA"
                     /locus_tag="ACHKYO_RS09310"
                     /old_locus_tag="pgaptmp_001862"
     CDS             complement(11079..12653)
                     /gene="nikA"
                     /locus_tag="ACHKYO_RS09310"
                     /old_locus_tag="pgaptmp_001862"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4428719.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12764..13351)
                     /gene="acpT"
                     /locus_tag="ACHKYO_RS09315"
                     /old_locus_tag="pgaptmp_001863"
     CDS             complement(12764..13351)
                     /gene="acpT"
                     /locus_tag="ACHKYO_RS09315"
                     /old_locus_tag="pgaptmp_001863"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HGU4428720.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13406..14455)
                     /gene="yhhT"
                     /locus_tag="ACHKYO_RS09320"
                     /old_locus_tag="pgaptmp_001864"
     CDS             complement(13406..14455)
                     /gene="yhhT"
                     /locus_tag="ACHKYO_RS09320"
                     /old_locus_tag="pgaptmp_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU4428721.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14587..15804
                     /gene="yhhS"
                     /locus_tag="ACHKYO_RS09325"
                     /old_locus_tag="pgaptmp_001865"
     CDS             14587..15804
                     /gene="yhhS"
                     /locus_tag="ACHKYO_RS09325"
                     /old_locus_tag="pgaptmp_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4428722.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15808..16365)
                     /gene="dcrB"
                     /locus_tag="ACHKYO_RS09330"
                     /old_locus_tag="pgaptmp_001866"
     CDS             complement(15808..16365)
                     /gene="dcrB"
                     /locus_tag="ACHKYO_RS09330"
                     /old_locus_tag="pgaptmp_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HGU4428723.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16438..17103)
                     /gene="yhhQ"
                     /locus_tag="ACHKYO_RS09335"
                     /old_locus_tag="pgaptmp_001867"
     CDS             complement(16438..17103)
                     /gene="yhhQ"
                     /locus_tag="ACHKYO_RS09335"
                     /old_locus_tag="pgaptmp_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HGU4428724.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            17324..17569
                     /gene="tusA"
                     /locus_tag="ACHKYO_RS09340"
                     /old_locus_tag="pgaptmp_001868"
     CDS             17324..17569
                     /gene="tusA"
                     /locus_tag="ACHKYO_RS09340"
                     /old_locus_tag="pgaptmp_001868"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HGU4428725.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17671..19869)
                     /gene="zntA"
                     /locus_tag="ACHKYO_RS09345"
                     /old_locus_tag="pgaptmp_001869"
     CDS             complement(17671..19869)
                     /gene="zntA"
                     /locus_tag="ACHKYO_RS09345"
                     /old_locus_tag="pgaptmp_001869"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HGU4428726.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLQLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19943..20569)
                     /gene="yhhN"
                     /locus_tag="ACHKYO_RS09350"
                     /old_locus_tag="pgaptmp_001870"
     CDS             complement(19943..20569)
                     /gene="yhhN"
                     /locus_tag="ACHKYO_RS09350"
                     /old_locus_tag="pgaptmp_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HGU4428727.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20710..21069
                     /gene="yhhM"
                     /locus_tag="ACHKYO_RS09355"
                     /old_locus_tag="pgaptmp_001871"
     CDS             20710..21069
                     /gene="yhhM"
                     /locus_tag="ACHKYO_RS09355"
                     /old_locus_tag="pgaptmp_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HGU4428728.1"
                     /translation="MSKPPLFFIVIICLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFSLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(21072..21341)
                     /gene="yhhL"
                     /locus_tag="ACHKYO_RS09360"
                     /old_locus_tag="pgaptmp_001872"
     CDS             complement(21072..21341)
                     /gene="yhhL"
                     /locus_tag="ACHKYO_RS09360"
                     /old_locus_tag="pgaptmp_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HGU4428729.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(21331..21927)
                     /gene="rsmD"
                     /locus_tag="ACHKYO_RS09365"
                     /old_locus_tag="pgaptmp_001873"
     CDS             complement(21331..21927)
                     /gene="rsmD"
                     /locus_tag="ACHKYO_RS09365"
                     /old_locus_tag="pgaptmp_001873"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HGU4428730.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            22077..23573
                     /gene="ftsY"
                     /locus_tag="ACHKYO_RS09370"
                     /old_locus_tag="pgaptmp_001874"
     CDS             22077..23573
                     /gene="ftsY"
                     /locus_tag="ACHKYO_RS09370"
                     /old_locus_tag="pgaptmp_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGU4428731.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23576..24244
                     /gene="ftsE"
                     /locus_tag="ACHKYO_RS09375"
                     /old_locus_tag="pgaptmp_001875"
     CDS             23576..24244
                     /gene="ftsE"
                     /locus_tag="ACHKYO_RS09375"
                     /old_locus_tag="pgaptmp_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGU4428732.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            24237..25295
                     /gene="ftsX"
                     /locus_tag="ACHKYO_RS09380"
                     /old_locus_tag="pgaptmp_001876"
     CDS             24237..25295
                     /gene="ftsX"
                     /locus_tag="ACHKYO_RS09380"
                     /old_locus_tag="pgaptmp_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGU4428733.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25540..26394
                     /gene="rpoH"
                     /locus_tag="ACHKYO_RS09385"
                     /old_locus_tag="pgaptmp_001877"
     CDS             25540..26394
                     /gene="rpoH"
                     /locus_tag="ACHKYO_RS09385"
                     /old_locus_tag="pgaptmp_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HGU4428734.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26665..27768
                     /gene="livJ"
                     /locus_tag="ACHKYO_RS09390"
                     /old_locus_tag="pgaptmp_001878"
     CDS             26665..27768
                     /gene="livJ"
                     /locus_tag="ACHKYO_RS09390"
                     /old_locus_tag="pgaptmp_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HGU4428735.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27967..28350)
                     /gene="panM"
                     /locus_tag="ACHKYO_RS09395"
                     /old_locus_tag="pgaptmp_001879"
     CDS             complement(27967..28350)
                     /gene="panM"
                     /locus_tag="ACHKYO_RS09395"
                     /old_locus_tag="pgaptmp_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HGU4428736.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            28774..29883
                     /gene="livK"
                     /locus_tag="ACHKYO_RS09400"
                     /old_locus_tag="pgaptmp_001880"
     CDS             28774..29883
                     /gene="livK"
                     /locus_tag="ACHKYO_RS09400"
                     /old_locus_tag="pgaptmp_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HGU4428737.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            29931..30857
                     /gene="livH"
                     /locus_tag="ACHKYO_RS09405"
                     /old_locus_tag="pgaptmp_001881"
     CDS             29931..30857
                     /gene="livH"
                     /locus_tag="ACHKYO_RS09405"
                     /old_locus_tag="pgaptmp_001881"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HGU4428738.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALIMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTVFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            30854..32131
                     /gene="livM"
                     /locus_tag="ACHKYO_RS09410"
                     /old_locus_tag="pgaptmp_001882"
     CDS             30854..32131
                     /gene="livM"
                     /locus_tag="ACHKYO_RS09410"
                     /old_locus_tag="pgaptmp_001882"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HGU4428739.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            32128..32895
                     /gene="livG"
                     /locus_tag="ACHKYO_RS09415"
                     /old_locus_tag="pgaptmp_001883"
     CDS             32128..32895
                     /gene="livG"
                     /locus_tag="ACHKYO_RS09415"
                     /old_locus_tag="pgaptmp_001883"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HGU4428740.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            32897..33613
                     /gene="livF"
                     /locus_tag="ACHKYO_RS09420"
                     /old_locus_tag="pgaptmp_001884"
     CDS             32897..33613
                     /gene="livF"
                     /locus_tag="ACHKYO_RS09420"
                     /old_locus_tag="pgaptmp_001884"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HGU4428741.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGGQ"
     gene            34006..35322
                     /gene="ugpB"
                     /locus_tag="ACHKYO_RS09425"
                     /old_locus_tag="pgaptmp_001885"
     CDS             34006..35322
                     /gene="ugpB"
                     /locus_tag="ACHKYO_RS09425"
                     /old_locus_tag="pgaptmp_001885"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HGU4428742.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            35420..36307
                     /gene="ugpA"
                     /locus_tag="ACHKYO_RS09430"
                     /old_locus_tag="pgaptmp_001886"
     CDS             35420..36307
                     /gene="ugpA"
                     /locus_tag="ACHKYO_RS09430"
                     /old_locus_tag="pgaptmp_001886"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HGU4428743.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            36304..37149
                     /gene="ugpE"
                     /locus_tag="ACHKYO_RS09435"
                     /old_locus_tag="pgaptmp_001887"
     CDS             36304..37149
                     /gene="ugpE"
                     /locus_tag="ACHKYO_RS09435"
                     /old_locus_tag="pgaptmp_001887"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HGU4428744.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            37151..38221
                     /gene="ugpC"
                     /locus_tag="ACHKYO_RS09440"
                     /old_locus_tag="pgaptmp_001888"
     CDS             37151..38221
                     /gene="ugpC"
                     /locus_tag="ACHKYO_RS09440"
                     /old_locus_tag="pgaptmp_001888"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HGU4428745.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            38218..38961
                     /gene="ugpQ"
                     /locus_tag="ACHKYO_RS09445"
                     /old_locus_tag="pgaptmp_001889"
     CDS             38218..38961
                     /gene="ugpQ"
                     /locus_tag="ACHKYO_RS09445"
                     /old_locus_tag="pgaptmp_001889"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU4428746.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGESLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(38948..39388)
                     /gene="yhhA"
                     /locus_tag="ACHKYO_RS09450"
                     /old_locus_tag="pgaptmp_001890"
     CDS             complement(38948..39388)
                     /gene="yhhA"
                     /locus_tag="ACHKYO_RS09450"
                     /old_locus_tag="pgaptmp_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HGU4428747.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            39508..41250
                     /gene="ggt"
                     /locus_tag="ACHKYO_RS09455"
                     /old_locus_tag="pgaptmp_001891"
     CDS             39508..41250
                     /gene="ggt"
                     /locus_tag="ACHKYO_RS09455"
                     /old_locus_tag="pgaptmp_001891"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HGU4428748.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(41288..41572)
                     /gene="yrhB"
                     /locus_tag="ACHKYO_RS09460"
                     /old_locus_tag="pgaptmp_001892"
     CDS             complement(41288..41572)
                     /gene="yrhB"
                     /locus_tag="ACHKYO_RS09460"
                     /old_locus_tag="pgaptmp_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HGU4428749.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         9759 a        10742 c        11638 g         9634 t
ORIGIN      
        1 ttgtttattt acttaataat atgacagtaa atgatgatgc taatgatgtt gcagttactg
       61 agtcgtacaa acccgtctgg gactggaaaa tatcgcaatt caatgtatct gatattaaat
      121 tcgaaacaat gataaacccc cagttcgctg acagaaccta tttctccaat tgttcacctg
      181 ttgatcccac aagtaccaga ccaacctatt ttggcgatac cgatggctca gtcggtgcag
      241 ttttgtacgc gctttttgca acgggacatt tgagaatcat ggctgaagga gaaaatttcc
      301 ttagccagtt gcttaatata gaagatgaag ttctcaacat attgctaagg gagaacttta
      361 atgagcaact tgacactaat gttaatacaa taatcagtat tctgaacaga agagatatag
      421 ttttagaaag tcttcagcct tatttagtta ttaataaaga cgctgtgacg ccatgtacgt
      481 ttcttggcga ccaaaccggc gatcgattta gtaatatctg tggcgatcaa tttatcatcg
      541 atttgttaaa acgcataatg agtatcaatg aaaatgttca tgtattagct ggaaatcatg
      601 aaacgaattg caatggtaac tatatgcaga acttcacgcg tatgaaacca cttgatgagg
      661 atacctacgc tgggataaaa gattatcctg tatgtttcta tgaccccaaa tacaaaataa
      721 tggcaaatca tcatgggata acttttgatg agcagcgaaa acgttacatt ataggaccga
      781 taacagtctc tattgatgaa atgaccaatg ctctcgatcc ggtcgaatta gcggcaatta
      841 tcaataaaaa gcatcatgct ataattaatg gcaaaaagtt taaaactagc cgggctatct
      901 cttgtcgatc ctttaatcgt tatttttctg tttcgacaga ttacagacct aaacttgaag
      961 cactattagc atgttcacaa atgttaggta taaatcaagt tgttgcacat aacggaaatg
     1021 gtggaagaga acgcataggc gaaacgggga cagttttagg tcttaacgcc cgtgacagta
     1081 aacatgctgg aagaatgttc agcatgcata attgccaaat taaccctggt gcagggcctg
     1141 agataaccac tccctggaag tcttaccagc atgaaaaaaa cagaaatgga cttatgcctt
     1201 tgattagaag gcgcacaatg ttacagctct gaaccataga caacatgaat attaatgtta
     1261 ctatctggtt gagactgttg ataaaacgta aaaaggaatg ctctgttcct gcaactgaac
     1321 aggcgtgaaa cgctatccaa caggatggat accgttttgt cagccagttt tatagatcac
     1381 acttatgagt atgcgtgttt catttacggc caggaatgct gatgatgaca acattactac
     1441 ccgttttcac taagccttcc ccgttggcgc tcaatgctct gcgcgctggt cgtatttgcc
     1501 gtttccttct tatcccggat ggaagaatcc gctgaacgtc gttcggcggc agttgtaaca
     1561 cgcgtcgttt gcttacaggg attatggcgg gtcgattatg gataagagta agcgccatct
     1621 ggcgtggtgg gttgtcgggt tactggcggt ggcggctatc gtggcgtggt ggctgttgcg
     1681 cccggcaggt gtgccggaag gctttgctgt cagcaatggg cgcattgaag cgacggaagt
     1741 ggatatcgcc agcaaaattg ccgggcgtat tgacaccatt ctggtgaaag aaggccagtt
     1801 tgttcgagaa ggtgaagtgc tggcgaagat ggatactcgc gtgttgcagg aacagcgact
     1861 ggaagccatc gcgcaaatca aagaagcaca aagcgccgtt gctgccgcgc aggctttgct
     1921 ggagcaacgc caaagtgaaa ctcgcgccgc tcagtcgctg gttaatcaac ggcaagctga
     1981 actggactcc gtagccaaac gtcatacccg ttcccgctcg ctcgcccatc gtggggctat
     2041 ttctgcgcaa cagttggatg acgaccgcgc cgccgctgag agcgcccgag ctgcgctgga
     2101 atcggcaaaa gctcaggttt cggcttctaa agccgctata gaagcggcac gcaccaatat
     2161 cattcaggcg caaactcgcg tcgaagccgc acaagccact gaacggcgca ttgccgcaga
     2221 tatcgatgac agcgaactga aagccccgcg tgacggacgc gtgcagtatc gggttgccga
     2281 gccaggcgaa gtgctggcgg caggcggtcg ggtgctgaat atggtcgatc tcagcgacgt
     2341 ctatatgact ttcttcctgc caaccgaaca ggcgggcacg ctgaaactgg gcggtgaagc
     2401 ccggctgatc ctcgatgccg cgccagatct gcgtattcct gcaaccatca gttttgtcgc
     2461 cagtgtcgcc cagttcacgc caaaaaccgt cgaaaccagc gatgaacggc tgaaactgat
     2521 gttccgcgtc aaagcgcgta tcccaccgga attactccag cagcatctgg aatatgtcaa
     2581 aaccggttta ccgggcgtag cgtgggtgcg ggtgaatgaa gaacttccgt ggcctgacga
     2641 cctcgtggtg aggttgccgc aatgacgcat ctggaactgg ttcccgtccc gcctgtcgcg
     2701 caactggcgg gcgtgagcca gcattatgga aaaaccgttg cgctgaacaa tatcacactc
     2761 gatattccgg cccgttgcat ggtcgggctg attggcccgg acggcgtcgg gaagtcgagc
     2821 ttgttgtcgt tgatttccgg tgcccgcgtc attgaacagg gcaatgtgat ggtgctgggc
     2881 ggcgatatgc gcgacccgaa gcatcgccgc gacgtctgcc cgcgcatcgc ctggatgccg
     2941 caggggctgg gcaaaaatct ctaccacacc ttatcggtgt atgaaaatgt cgattttttc
     3001 gctcgcttgt tcggtcacga taaagcggag cgggaagtcc gaattaatga gctgctgacc
     3061 agcaccgggt tagcaccgtt tcgcgatcgt ccggcaggta aactctccgg cgggatgaag
     3121 caaaaactgg ggctgtgctg cgcgttaatc cacgacccgg aactgttgat ccttgatgag
     3181 ccaacaacgg gggttgaccc gctctcccgc gcccagttct gggatctgat cgacagtatt
     3241 cgccagcggc agagcaatat gagcgtgcta gtcgccaccg cctatatgga agaggccgaa
     3301 cgcttcgact ggctggtagc gatgaatgcc ggagaagtgc tggcaactgg cagcgccgaa
     3361 gagctgcggc agcaaacgca aagcgccacg ctggaagagg cgtttataaa tctgttaccg
     3421 caagcgcaac gccaggcgca tcaggcggta gtgatcccac cgtatcaacc tgaaaatgca
     3481 gagattgcca tcgaagcgcg cgatctgacc atgcgttttg gttccttcgt tgccgttgat
     3541 cacgtcaatt tccgcattcc acgcggggag atttttggtt ttcttggatc gaacggctgc
     3601 ggtaaatcca ccaccatgaa aatgctcacc ggactgctgc ccgccagcga aggtgaggcg
     3661 tggctgtttg ggcaaccggt tgatccaaaa gatatcgata cccgccgtcg ggtgggctat
     3721 atgtcgcagg cgttttcgct ctataacgaa ctcaccgtgc ggcaaaacct tgagttacat
     3781 gcccgtttgt ttcacatccc ggaagcggaa attcccgcgc gagtggctga aatgagcgag
     3841 cgttttaagc tcaacgacgt tgaagatgtt ctgccggagt cattgccgct cggcattcgc
     3901 cagcggcttt cgctggcggt ggcggtgatt catcgcccgg agatgttaat cctcgatgaa
     3961 cctacttctg gcgtcgatcc ggtggcgagg gatatgttct ggcagttgat ggtcgatctc
     4021 tcgcgccagg acaaagtaac catttttatc tccacccact ttatgaacga agcggaacgt
     4081 tgcgaccgca tctcactgat gcacgccgga aaagtgctcg ccagcggtac accgcaggaa
     4141 ctggttgaga aacgcggagc cgccagtctg gaagaggcat ttatcgccta tttgcaggaa
     4201 gcggcaggac agagcaacga agccgaagcg ccgcccgtga tacacgacac cacccacgcg
     4261 ccgcgtcagg gatttagcct gcgccgtctg tttagctaca gccgccgcga agcactggaa
     4321 ctgcgacgcg atccggtacg ttcgacgctg gcgctgatgg gaacagtgat cctgatgctg
     4381 ataatgggtt acggcatcag tatggatgtg gaaaacctgc gctttgcggt gctcgaccgc
     4441 gaccagaccg tcagtagcca ggcgtggacg ctcaatctct ccggttcccg ttactttatc
     4501 gaacagccgc cgctcaccag ttatgacgag cttgatcgtc ggatgcgtgc gggcgatata
     4561 acggtggcaa ttgagatccc tcccaatttc gggcgcgata tcgcgcgtgg tacgcctgtg
     4621 gaactcggcg tctggatcga cggagcgatg ccgagccgtg ccgaaacggt aaaaggttac
     4681 gtgcaggcaa tgcaccagag ctggctacag gatgtggcga gccgacaatc cacacccgcc
     4741 agccaaagcg ggctgatgaa tattgagacg cgctatcgct ataacccgga cgtaaaaagc
     4801 ctgccagcga ttgttccggc ggtgatcccg cttctgctga tgatgatccc gtcaatgcta
     4861 agcgccctta gcgtggtgcg ggaaaaagag cttgggtcga ttatcaacct ttacgtgacc
     4921 cccaccacgc gtagtgaatt tttgcttggt aaacagttgc catacatcgc gctggggatg
     4981 ctgaactttt tcctgctctg cggcctgtcg gtgtttgtgt ttggcgtacc gcataaaggc
     5041 agtttcctga cgctcaccct ggcggcgctg ctgtatatca tcattgccac cggaatgggg
     5101 ctgctgatct ccacctttat gaaaagccag attgccgcca ttttcggaac ggcgattatc
     5161 acgttgatcc cggcgacaca gttttccggg atgatcgatc cggtagcttc gctggaaggg
     5221 cctggacgtt ggatcggcga ggtttacccg accagtcatt ttctgactat cgcccgcggg
     5281 acgttctcga aagcgctgga tctgactgat ttgtggcaac tttttatccc gttgctgata
     5341 gccatcccgc tggtgatggg cttaagtatc ctgctgctga aaaaacagga gggatgatgc
     5401 gccatttacg caatattttt aatctgggta tcaaagagtt gcgcagtctg ctcggtgata
     5461 aagcgatgct gacgctgatt gtcttctcgt ttacggtgtc ggtgtattcg tcagcgaccg
     5521 ttacgccagg atcgttgaac ctcgcgccga tcgccattgc cgatatggat caatcgcagt
     5581 tatcgaaccg gatcgttaac agcttctatc gtccgtggtt tttgccaccg gagatgatca
     5641 ccgccgatga gatggatgcc ggactggacg ccggacgcta taccttcgcg ataaatattc
     5701 cgcctaattt tcagcgtgat gtcctcgccg gacgccagcc ggatattcag gtgaacgtcg
     5761 atgccacgcg catgagccag gcatttaccg gcaatgggta tatccagaat attatcaacg
     5821 gtgaagtgaa cagctttgtc gcgcgctacc gtgataacag cgaaccgttg gtatcgctgg
     5881 aaacccggat gcgctttaac ccgaacctcg atcccgcgtg gtttggcggg gtgatggcga
     5941 tcatcaacaa cattaccatg ctggcgattg tattgaccgg atcggcgctg atccgcgagc
     6001 gtgaacacgg cacggtggaa cacttactgg tgatgccgat aacgccgttt gagatcatga
     6061 tggcgaagat ctggtcgatg gggctggtgg tgctggtggt atcgggatta tcgctggtgc
     6121 tgatggtgaa aggtgtactg ggcgtaccga ttgaaggctc gatcccgctg tttatgctgg
     6181 gcgtggcgct cagtctgttt gccaccacgt caatcggcat ttttatgggg acgatagcgc
     6241 gttcaatgcc gcaactgggg ctgctggtga ttctggtgct gctgccgctg caaatgcttt
     6301 ccggtggatc cacgccgcgc gaaagtatgc cgcagatggt gcaggacatt atgctgacca
     6361 tgccgacgac acactttgtt agcctcgcgc aggccattct ctaccggggt gccggattcg
     6421 aaatcgtctg gccgcagttc ctgacgctga tggcaattgg cggcgcgttt ttcaccatcg
     6481 cgctgctgcg attcaggaag acgattggga caatggcgta aggaagggaa acttgacttc
     6541 atactgtgat gtcataaagt gaagtcataa gggaatgtca tgtaaacaca ggtacaggct
     6601 ttacgaaaga aacaaaaaaa cacactggat cagctattta aatcaccttt acctcaagga
     6661 atcaaatgga ctgatataga atctctggtt aaagcgttag gcggagaaat taaggaaggt
     6721 agggggtcgc gctgtaagtt catactaaat atgagcgttg cgtgtttcca tcggcctcat
     6781 ccgtcgccag ataccgataa aggcgctgta gaaagcgtgc gtgactggtt actaagtata
     6841 ggggtaaaac catgatcaaa ctgaagacgc caaactttat ggagattgcc gggcaacctg
     6901 ctgttatcac ttacgtgcca gaactgaatg catttcgtgg caaattcctg ggcttgtcag
     6961 gttattgtga ttttgtctct gacagcattc aggggctgca aaaagaaggg gaactttccc
     7021 tgcgagagta tcttgaggat tgtaaagctg cgggtattga gccgtatgcg cgtacagaaa
     7081 agattaagac ctttacgtta cgctatccag aatcgctgag tgaacgcctt aataatgcgg
     7141 ctgcgcagca acaagtctct gttaatacct atattattga aacactgaat gagcgtttga
     7201 atcatttata aattttagcg gcgtacatta cgcagaatat ctgcctttac aggatctatc
     7261 tatgcaactg aacttaattt tataaattaa gttcagttaa tatcttttct ttcctcgttt
     7321 cagaaataac tcaatcttcc ttcggcaggc actgcaaatg cccgtgccgc acgccgcgct
     7381 gggcgataac gtcatcggca aaatgctgca cgtcgcccat atcacctttc agcacggcga
     7441 tttccagaca gtcgtcgtga ttgatatgca catgcagcgt ggcgacggag agatcgtggt
     7501 gatggtgctg ggtggagaca atgcggctgg ctaagtcgcg tttttcgtgt tcatacacat
     7561 aggagagcac cgcgaaacct tgcgtgccgt gctgctgagt ggcctcctgg gccagggcgc
     7621 tacgcagaat atcgcggata gcttcggaac ggttgttata accacgacgc tggctcaggc
     7681 tgtccagcgt ctccagtaaa tcgtcatcaa gcgtgatggt gactcgttgc atttgcgtta
     7741 aaccttttct gtggtgcgac ggcgcacggg gaatgcgggt aataccgcgt tttgtagcac
     7801 acgtccggcg tcagaggaaa aggttaattt atctcccacc acctgggttt cgacgatttg
     7861 tccgttgtcc atcaccatca cccgctgaca gaagcgttcc accaggcgta agtcgtgggt
     7921 gatgaacagg caggcggtgc caaactgttg ttgtagcttt ttcagcaggc gaatgacgcc
     7981 cgcctgtaac acgagatcaa ggttagaaac ggcttcatcc agaatcagta gtttcggttc
     8041 gaccgccagc gcgcgagcca ggcagacgcg ctggagctgg ccgccgctta actgcggtgg
     8101 gcgtttgtcg agaacgctgt catcgagatc gaccgccttc agcatttcgc tggcgcgcgc
     8161 cagttgttcg gattttttca gtgatagcag gtggcgcatc ggttcacgca ggatctcgcg
     8221 cacggttttg cgcggattca cggcgctgat ggagtcctga aataccatct gaatatcgcg
     8281 gcggaacgct ttacgctggg cgcgattgag tttcgccagc ggttcgccgc gccagctaat
     8341 attcccttgt gagggagact ctaagcctac cagcagccgc gcgagggtgc tttttccgca
     8401 gccgctgcgt cccagcaggg cgacggtttc gccgcttttc agggtcaggg aaacgttatt
     8461 cagcaccgcc tgatgttgat gttttccgct aaatccaccg tgggcatagt gatgggaaag
     8521 gccgcagacg ttaagtaagg tcatgatgcc agctccatac cgtagagggc gagatgagcg
     8581 gaaaccaggc tgcgcgtcac cgtatgtttg ggggcgttaa acagcgtttc tacatcgccc
     8641 tgttcgacaa ttttaccttg tgacatcacc gccacgtcat ccgccagacg cgccacaacg
     8701 cccatatcat gggtcaccag cagcattccc ggcgcttgtt tttgcataat gctttccagc
     8761 agatcgagga tgcgcgcctg tgctaccacg tcgaggtcgg tggtcggttc atcggcgatg
     8821 ataaacggtg attcacacag caccgccatc gcaatcatca tgcgctgcaa catgccgccg
     8881 ctcatctcga acgggtacag cttcagcacg cgcgcggcgt tttccagccc caccgcttct
     8941 atggcagcgg taagcgtagc gtcatcggcg ggtttcccta acgccaggca ggtttcccgc
     9001 gcgtgggtgt gcatggtgtg cagtggatta aaggcgctgc gcgggttctg catgatggtg
     9061 gcaattttga tgccgcgcag ggcgcaaggc gaaaccggtt tgccatcggc taaaatttcc
     9121 cccgccgtct ggcgaacgcc agcgggcaaa atgcccagcg tcgcggcgca ggttaatgat
     9181 ttcccgctgc cgctaccgcc gactaacgcc agcacgcgcc cgcgttgcag ggttaacgat
     9241 acaccgtgta ccagcggctg cgcggcctgt agcgcgatat tacgtagttc aatctgttgc
     9301 ggcattagtg tgcgtgctcc gtcaccagat gaggatccag atgatcgcgc agtgcgtcac
     9361 ccaccaggtt aaaggccatc acgctgataa acagcgccag ccccggccag aacatttgca
     9421 gcggctgggt ccagatatac tggcgcgcgt cgttaatcat cacgccccat tcggcggtcg
     9481 gcgcggtcac accgaggcca aggaaagaca tccccgcgac gtgcagcatc atatggccga
     9541 tatccagcgt tgccagcacc agcagcgaag ggatcaccgc gcctgccaga tgatcgacaa
     9601 acacccgcac atggcccgca ccggaaagcc gtgacgccag cacaaactcg cgttggcgta
     9661 gtgaaatcac caggctgcgc accatgcgtg cataccacgc ccagtgcgac agggcgatgg
     9721 cgataattac gttggtcagc ccggtgccga gcacgccaac cataaagaac gacagaatcg
     9781 aggtcgggaa ggtcataaac atatcggcga cgcgcatggt ggcctgatca acgcgtccgc
     9841 caatcagtcc ggcgctgccg ccaatcacca gccctaatgt cagcaccagc agcaggcagg
     9901 ccattaccga accgagcgac acgcgggtcg ccgccatcag ccgcgagaaa atatcacgcc
     9961 ctaagtgatc ggtgcccagc cagtgctgcg catccggcga aagcaggcgc gacggcagat
    10021 caatagcctg agggtcatac ggcagccacc actggctggt aagcgcaatc agcgccagca
    10081 gggcgataat gatcagcgcc aggcgtaccg accagcggga agagaggaaa aagttcacgc
    10141 gtgcgctcct tcatgacgac gaatgcgcgg gtccagcgcg gcgttgagca aatcgacaat
    10201 caaattacag accacaaaaa ccaccaccat catcagcgta aagcactgga tcaccggata
    10261 gtcacggtta aaaatcgccg acaccgcata gcgcccgacg cccggccagg caaagatgtt
    10321 ttcgataatc atcgtcccgc cgatcagttc gccgatgtgc atccccacgg cggtgatcat
    10381 cggcagcgag gcattgcgca ggatgtggcg acgttcggtc tgtttgtcgt tcaggccgcg
    10441 cagacgcgcc caggtgacgt gacgctgacc ggcgacgtcc agcatactgg cgcgcagtaa
    10501 acgcgcgtta atcgccagcg acataaaggc aatggaaacc gcaggcaaaa tgatgtgctg
    10561 ccagccgccg taacccatcg cgggtagcca ttgcagatac accgaaaacg ccatcaccag
    10621 taaaaacgcc agccagaagt taggcatcga cacgccaaga aacgcgatga aacgtacggc
    10681 gaaatccggc agacggtcgc ggtggcgcgc cgcccagata ccgagcggta cggaagtgag
    10741 cagaattaat accagcgccg cacctgcaag ttccagcgtg gcgggcagga agttcagcat
    10801 atcgtccagt accgggcgtt ggctggcgaa tgagatacca aagtcaagat gcagcgcctt
    10861 ccacaaccag gtgccgtact ggacgtacag cggctgatcc agtcccagca tggtgcgggt
    10921 agaggccagc atctccggcg tcggcggcag gttagacaga cgcaaataat cgagcgccgg
    10981 gtcgccggta ccgaggcgca gcatcagaaa aatgatcacc gaggcggcga gcaccatcgg
    11041 gatcagcagc agaaagcggc gtaatacgta acgcaacatt aaggtttcac cggtttaatc
    11101 tgttcgaacg gaatttcggt ggcgatcggc gcgtagggga tattacccag ctccggtttt
    11161 gataccacca tcatcgagat gtagctgata ggcagataaa ccgcctcgtc atgcagacgg
    11221 gtcagaatgt cgcgatacag cgcctgacgt tgcgtttcgt catgggtcgc cagcacttcg
    11281 ccgatctctt tatcaatcag cggcttatcg gctaatcctt gctgtgcctg gaagtcagcg
    11341 tgcgacggta cgcgcattga actcaggaag gcgtgtggat cgtatggcgc gccccaggtg
    11401 cggtggaaaa tcatgccaaa acgaccgtcg cgctgacgag catagatact gctctcttct
    11461 tcgccaatca gcgagacatc tgcgccaatc tggcgcatat cagcctgaat gatttccgcc
    11521 atcgatttgc ttaacgcatc ggtgccgatg aacgacagtt caatgcgcag cggctgacca
    11581 tttttctcgc ggatgtcttt gcccgcaggc agcgtccaac cggctttttc cagcaacgct
    11641 ttcgctttct gcggatcgta ctggcgcggt ttcaggccga gattagcgta aggcacagaa
    11701 ggggcaaaca gggtgtcggc gacctgctgg gtgccatata acgcgttatc aatcagcgat
    11761 tttttgttta ccgcgtaatt aagagcttca cgtactgcca gctcgttggt gggggctttg
    11821 gcggtattga gcgccagcat cacggtttcg atcggctgtg acagttgggt gtggtaagcc
    11881 ggattctggc taaagcgggc gaaggtatcg agcggtaata acccttcgtt tccgtacagc
    11941 aggtcgatat cgccagtttc aaacgccacc gcgcgggtag tcgggtccgg gatgacgtta
    12001 aaggtgatct ttttaatcgc tggcttttcg ccccagtagt tttcgttacg gacgaagaca
    12061 tcgtactgat tcagtttcga ttcctgcaaa atccatggtc cggtgccaat cggcgcttta
    12121 attccgttca tggtttcatg gtttttaaac tgcgagggag cgataaaacg gaaaggacgg
    12181 ggcagggcca gttcttgcag gaaaggatag taggcgcttt tcagggtaat ttgcagctct
    12241 gttttactga gtgctttaac atcaacaatc tggtttgcca gctccagcca ggcgtgacgt
    12301 tgacggttat cgagcactgc gcggaagttt tctgccgccg cctcggcatc gaacggttca
    12361 ccgttggaga atttcacgtc atcacgcagg gtgaaggtcc aggttttacc atcttctgaa
    12421 tgagtccagc tttttgccag ccacgggatc accgaaccgt ctgcctgata tttcaccaat
    12481 ggttcataaa ccatgctctg ggcgaacatc tggttaggcg tgtaaaggtg cgggtttagt
    12541 ggcccgacat tcaccggcca ggcggtagtg atttcatctg gtgcagcggc atggacgata
    12601 aaagacgcac aagccagcag cgcaaataga gtgcggcgga gtgtggagag catgataacc
    12661 ccaatggatt aaaatagatg gcggaaataa gtatgacgat tttaagtatt cgtcatactg
    12721 attacctgtt ctggatcaat agatgggcag tcgggtgggc ggatcagtta actgaatcga
    12781 tccattgcac actgtcggcg gtgagcgtaa agggggtggg ggtgcagatc gccaggctta
    12841 aattttcaag ctgacaatgg ctgactgaca gcgaggagtg ataggtgctg tctacgctga
    12901 cgatttgcca ggcgctgccg ccacgctgtt taacgatggc ttctttgcgc gtccagatgc
    12961 gccagaacat ttccagttgc tgatcaggat gcacggcgtc catctcagcg tgttccccga
    13021 ggctgaatac ggcgttcgcc agccagcgcc agttggcgcg cgggcgaatc acttcgatat
    13081 cgcagccgac ttcgccttca tcactcaaca gcagagcgat atcgtcaccg ctatggctta
    13141 agttgaacca tagcggcgtt tccggcgcga atgcaggttt gccttgttcg ccatagatga
    13201 tctccggtag cggggaaagc gtgtgcgaaa gcaatgcacg ccccgccagc cagcgttcgc
    13261 gtcgtggacc ttgcggtgct tgctcgcgta aacccggtgg cagtggagct gcgcttaagg
    13321 tcgaaacttt ccccagaact atccgataca tatcagggcc aacgtttaat ggaaaatgaa
    13381 agtgcgtatc gtatcacttg tcgcctcatc ccggtaaccg acttttcggt ctgcccggcc
    13441 ccagtaaaat cgccagtttg ctgccacctt tcgtcgtttc catccagatt ttacacacgc
    13501 tggttaatgg caccgaaagt agcatcccta ccgggccgag cagccatccc cagattaaca
    13561 atgaaagaaa taccaccatg gtggacatcc ccaggcgatg gcccatcatc cgtggctcta
    13621 aaatattgcc gatgaccata tggacaacta aaaacaatgc gccgaccaga atacattcgt
    13681 aaataccatt aaacagcagc acctgaatca ttggcggtac ggcggaaatt accgcgccga
    13741 tattgggcac gtagttaagc aaaaacgcca gtactgccca catcagcgca aactgtaccc
    13801 ccatcagctc cagccccagc cagacgatga cacctgtcca taaactgagt agcgtcttca
    13861 atgcaagata gtgcgaaacg cctttaagtg cgcggtgtaa tcccgcgatg tgaatctgtg
    13921 gattattcag cgcaaaacgc attttgtaag ggacgtggcg cacttcaaac agcataaaaa
    13981 ctacggtcat caccagcaaa agcacgctcg ccattgcccc ggaaagcccg gtcattagcg
    14041 ctgtggtgaa ggtaaccact ttttccgagt ccatccgctg cagcattcgc tccggcgaca
    14101 tatgcaaatt aagaaaaggc aacatctcct gcaatttaaa aagtttgcgc gtcagctcct
    14161 tattaaactt cggcagcata gagataaatt cgttaaacga tgccgccagt acgccgacca
    14221 gcgcggttag tgcgatcagc atcaccacca ctacaatcgt aatggcaacg gggcgttgta
    14281 ctccccgacg aataaaccag gtgacgagcg ggttgaggac gatggcaaaa aacagcgcca
    14341 gtaacagctg cacaatgata tctgccgctg cgtgaatgcc cgcgaggatc actaccagcg
    14401 aggccagctt gagcagaatg tgcatgcccg ttttatcggg ttgaggggtt tccattgggg
    14461 cttccttgtg actttttgta ttaagtgtag cgggagtcac gccagcaata ttctgattct
    14521 tactgctgat tttcacgtca gcacatggta aaaatgaaac actgttgtaa aaatgtggtg
    14581 atcctcatgc ccgaacccgt agccgaaccc gcgctaaacg gattgcgcct gaatttgcgc
    14641 attgtctcca ttgtcatgtt taacttcgcc agctacctca ccatcgggtt gccgctcgct
    14701 gtattaccgg gctatgtcca tgatgtgatg ggctttagcg ctttctgggc aggattggtt
    14761 atcagcctgc aatatttcgc caccttgctg agccgccctc atgccggacg ttatgccgat
    14821 ttgctgggac ccaaaaagat tgtcgtcttc ggtttatgcg gctgcttttt gagcggtctg
    14881 gggtatctga cggcaggatt aaccgccagt ctgcctgtca tcagcctgtt attactttgc
    14941 ctggggcgcg tcatccttgg gattgggcaa agttttgccg gaacgggatc gaccctgtgg
    15001 ggcgttggcg tggttggctc gctgcatatc gggcgggtga tttcgtggaa cggcattgtc
    15061 acttacgggg cgatggcgat gggtgcgccg ttaggcgtcg tgttttatca ctggggcggc
    15121 ttgcaggcgt tagcgttaat cattatgggc gtggcgctgg tggccatttt gttggcgatc
    15181 ccgcgtccga cggtaaaagc cagtaaaggc aaaccgctgc cgtttcgcgc ggtgcttggg
    15241 agcgtctggc tgtacggtat ggcgctggca ctggcttccg ccggatttgg cgtcatcgcc
    15301 acctttatca cgctgtttta tgacgctaaa ggttgggacg gtgcggcttt cgcgctgacg
    15361 ctgtttagct gtgcgtttgt cggtacgcgt ttgttattcc ctaacggcat taaccgtatc
    15421 ggtggcttaa acgtagcgat gatttgcttt agcgttgaga taatcggcct gctactggtt
    15481 ggcgtggcga ctatgccgtg gatggcgaaa atcggcgtct tactggcggg ggccgggttt
    15541 tcgctggtgt tcccggcatt gggtgtagtg gcggtaaaag cggttccgca gcaaaatcag
    15601 ggggcggcgc tggcaactta caccgtattt atggatttat cgcttggcgt gactggacca
    15661 ctggctgggc tggtgatgag ctgggcgggc gtaccggtga tttatctggc ggcggcggga
    15721 ctggtcgcaa tcgcgttatt actgacgtgg cgattaaaaa aacggcctcc ggaacacgtc
    15781 cctgaggccg cctcatcatc ttaaaactta ctgaataacc agcgtattaa tgatgttttc
    15841 tgcggtggtc tgcgcttttt gctgatcgtc agcgggcagc gtaatttgca tggtcagcag
    15901 ttgattaccc acgttaccca gaataacgga agagtacgcc gtctggcctt tcgcggagat
    15961 aatactgtct aactgctgca ttttgtgacc tttcagctca atggctttat tggttaccac
    16021 ttgcagctgc ggatcgcggc tacgttgctg atcttccaga cgcttcgcca gcaccgccag
    16081 atcttctttc ggatcatcgc ccatgatgac gatgactgct ttctgcccgg tggcgtcgga
    16141 ccagacatgc atgttattgg cctgcgttcc cagcttaccg ctctggtcgg tcatatccgc
    16201 tggcagcgag aaacttaact tgccatcaag caggttgacg ggattcccgg tagcgttact
    16261 ttccgcgacc gaaccctgcg ccgtagcgtt agtgtcttta tcatcacagg ccgcaagccc
    16321 cataaccagc aggccaattc cgacatattt aaccagattg cgcattgact tcttcctttc
    16381 gataaacggc cataacggct cattcatcca tcttatcaca actctgataa cgaaccttta
    16441 actcgcctgc aaagcgttga tttcggattt atctgccagt cttttcaaca gcatattgag
    16501 taatacgcca tacattggca ggaagaaaac gatactgatt aacactttga aacagtaatc
    16561 gaccagcgcg atttccatcc agtgttcagc cataaaggca tccgggctac gccagaaggc
    16621 aatgaagaaa aaggccagcg tgtcactgac gttaccgaac agtgtggacg ctgtcggtgc
    16681 cagccaccag cggcgacttt gacgcaggcg gttaaaaacg tgcacgtcga ggatttgccc
    16741 cagcgcgtag gccatgaaac tggcggtggc gatacgggcg acaaacaggt tgaagtgggc
    16801 gagtgcgccg aatccctgcc atgaacccat atagaacagc gacgagatga cataggagat
    16861 taataacgca gggatcatta ccgcgaagat aatgcgtcgg gccagcggtg cgccaaaaat
    16921 acgcacggtc aggtcggtcg cgagaaagat aaacggaaaa ctaaacgcgc cccaggtggt
    16981 atgaaaacca aaaatggaga ccggaagctg gaccagatag ttactggagg tgatcaccag
    17041 cagatgaaat aacgataacc agaacaacgc cttatagcgt tgagtttgcg agaaaacgtt
    17101 catattgtac ctttttgatt aaccattggg gtgagggaac ccaatacgta cgacacgtct
    17161 gttatcttaa cataataacg tgcttaccct tttttcgagc cgccgcatga tactgcttta
    17221 cgttgacaat gcaatggtta attttcacgc aatcgttaac ctggtttgct tacggactca
    17281 cagggcgtaa actagcgccg tttttttaag tgatgagaag aaaatgaccg atctcttttc
    17341 cagccctgac cacacactcg acgcgcttgg cctgcgctgc ccggaaccgg tgatgatggt
    17401 gcgcaaaacc gtgcgcaata tgcagcctgg cgaaacttta ctgattatcg ccgacgatcc
    17461 ggcgacgacc cgcgatattc ctgggttttg tacctttatg gaacacgaac tggttgcgaa
    17521 agagacggat ggactgcctt atcgttattt gattcgtaaa ggcggttgat ggggaaagat
    17581 tggcttcgat gccgcctttt cccctcaccc taaccctctc cccagaggag cgaggggacc
    17641 gatcgcgctc aatgttgcga tcggtttgcc ttatctcctg cgcaacaatc ttaacgcatt
    17701 cgctgttacc agcacggtcg caccggtatc tgccagcacc gccagccata gtccggtcat
    17761 ccctagcagc gtggtgacga ggaagatccc tttcagcccc agcgcaatag tgatgttctg
    17821 gcggatattg gcgtgagtgg cgcgtgccag ttcaatcatt tgcaccaggc cgcgcaggtg
    17881 gttatgggtt aatgctgcgt cggcggtttc cagcgccacg tctgtgccac tgcccattgc
    17941 aatcccaatg gcggcagctt tcatcgctgg cgcgtcgtta ataccatcac cgaccatcgc
    18001 caggggcgca tgttgattca gcttggtcac cgctttgact ttatcttccg gcaataggcc
    18061 cgctttaaac tccagcccca gctccccggc aattgccgcc gctgcgcgtg gattatcgcc
    18121 ggtgaggatc actcctttaa cccccagcgc gttcagttca ctgatggcag ttgctgcgtc
    18181 ggcgcgcagg gtatcctgca atgcaatgat acccagcacg tcatcattac gtactaccag
    18241 cacaaccgtt tgcccggcgc tttccagttc attaatcaaa ccagcaaatg catcagcggg
    18301 atgtttcccg gcagcgcaaa tcaatacgcg ctcaccgtta acctgcgctt caatgccaga
    18361 cccgaccagc gcccgctgtg attcggcggt gggaatggcg agttcagcaa cctgtgcttc
    18421 gcgtacgatg gcctgcgcca gtggatgcgt cgcgccttgc tcgaccgccg ccgccagtgt
    18481 cagcagttca gattcactaa tacccgttgc cggatgaatc gcggtaacgc gcggtttacc
    18541 gacggtcagc gtaccggttt tatcaaacgc cacctgagta acacgaccca gctgttccag
    18601 cgccgctccg cctttaatca acgccccacg acgcgctgcc gccgccagcc cggaggtaat
    18661 cgccgcaggc gtggagataa ctaacgcaca cgggcagcca atcagcagca gcgtcagccc
    18721 tttataaatc cactcctgcc agctggcggc aaacagcagc ggtggcacca gcgtcaccag
    18781 cagggcgacg gccataatcg ctggcgtata aatacggctg aaacggtcga taaaccgctc
    18841 aatgggggcg cggcgctctt cggcttcttc gatcagtttc agaatccggt caatggcgct
    18901 ggcacccggt tctgacagca cttccagcgt taccagacgg tctacgctgg tggctcctgc
    18961 aggaacttta tcgcccgtcg cgcgctccac cggaatggat tctccggtca gggcgctttc
    19021 atcaaaactg gcaaacggtg agagcagttt accgtcggca ggcaaacgcc cacctgcggc
    19081 gacttcaatc acatcgccag gccgcaggct gttaatcgcc acctcttccc gctcaccgtt
    19141 acgcaggcgc gtggcggttt ctggtttcag cgccattaac gcgctaacgc cctgacgcgc
    19201 gcggctggcg gcccagcctt ccagtcgttc accaatcaaa aacagcagca acaccatcgc
    19261 agcttcagcc gttgcgccaa taaacagtgc accaatagcg gctacgctca ttaaggtttc
    19321 aatggcgaag tagctgccgg atttgatcag ccgtaatgcc tgacgagcaa tcgggtacag
    19381 cccaaccagc gtggtcgcga taaacgccag ttgcccaaac ggatgattaa attgctccag
    19441 accccagctg attgccatca tcacgattag cgtaatcagc tgcagattct ctttcaggcg
    19501 cgatgcttgt ggctcgtcgg cggcctgctc atcgcgcagg gaatagcccg ctttttgcac
    19561 cgcagattca acctgggcac ggatgtcatt gtcggcatcg accaccagtt tttcggtggc
    19621 gaacagtacc tgcacctgat tcacacctgc aagctggcgc acggcatttt ctaccttgcg
    19681 cgcacaggcg gcgcagtcca tgccgctgac tttccagcta tagcgggtgc cggagacgtt
    19741 ttcagagagc gttggcgagc tggagcatgc gccgtcgcag caacagtcgt tggcgttctg
    19801 taccgtggtt agcggtttga acgcagcaaa ttgaggggct ttcttgccgt gattgtcagg
    19861 tgtcgacatg gcatcctccg gttaagtttt ttctcattaa ccgaaggata cactctggag
    19921 tcgactccag agtcaagttt tatcagagat acagtgagcg gacgatgagg aagtgaccgg
    19981 caaagtagca ggccgcagca atcgcgttat ccgcacggaa gcgtcggcga tagtggctcc
    20041 ccagccagac aaagttactg ataaacagca acgaagcgcc aacaaacgca gagagcgccg
    20101 gagcggtcgg acggaagaac cacagttcac ctgccagcca caccatcacc agcgtcatgc
    20161 cgataaacgt gcagataggc caacgatact cttccagccg cgtccagata atcgccagta
    20221 acagcgcacc cagaaccagc aacaccagtg gcagaggcca gaagaaagag agcgtcatct
    20281 gactggcgaa atagatggtg tacagcaggt gcgagaggaa aaacgcgccg atggcgtaca
    20341 tcagacgttg acgtggcaac agggttagcg catcgcccag cagtgaggcg cacagccctg
    20401 ccagcaccag atagctaatg gcgtcgaaca tcggcgcttg ccaggccagt aacagcagga
    20461 gaagaagggt taacggttta aacacccagc gttgccaggt tggcccacga tacgatgcat
    20521 ccacagatag ccatgcggaa agacagacag cgataaacga ccaaagcatc ttagttcctt
    20581 gaattcgtta ttttttcgta agcggaatgc ctacggtctt gcttccagtt tagtggcgag
    20641 ggtggacgga tgacaacacc ataactggca gggtatgctt atttccgcat tttccgatga
    20701 gggataaaga tgagcaaacc acctcttttc tttattgtta tcatttgctt aattgtcgtc
    20761 gccgcatcgt ttcgttttat gcaacagcga cgggaaaaag ctgataatga tatggctccg
    20821 ctccagcaaa agctggtggt ggtgagcaac aagcgggaaa aaccgattaa cgatcgccgt
    20881 tcgcgccagc aggaagtgac tccggcaggc accagtatgc gctatgaggc aagcttcaaa
    20941 ccgcaaagcg gaggaatgga gcagacgttt agcctcgacg cccagcagta ccacgccctg
    21001 acagtgggcg ataaaggtac gctgagctat aaaggaacgc gctttgtcag ctttgtaggc
    21061 gaacaataag gttatttttt aactttaaat ttcttctgcc agaccagcag ttcaaacacg
    21121 ccgaaaagga aaatccgcac cttttcggcg gtggtcatct gcgggccatc tttcggtaaa
    21181 gtggatttca atagcgccag ttgcatacca tgcatcagca cggtaaaaat cagcgccacg
    21241 ttaacgaaaa tattcagcgg gcgcgggaag ggatgcacca gattgaggat taaaaatccc
    21301 caaacgcaga gcattaacaa acgaccaata ttaatcagca tcactttctc cttgtgcttc
    21361 gcgttgatac agccgataag ccacctgacc cgccactttt tcccgatgta atgaccagtt
    21421 tgccggaaca gtgggcagac cgttttcgac ttcgctttcg acataaatca gggcttcgtc
    21481 agccagccag ccgttatctt ccagtaaatt tatcgtctct tctaacaagc cacggcggaa
    21541 cggtggatcg acaaacacga tattatgcgg tgtacctttt tgcgccagga atgacattgc
    21601 gttgctgttc accacgcgtg cattgcctgc ttttagtgtc gccagattct taattaactg
    21661 ctgagaaacc gcgcgatcca tctcaatcaa cgttgccccc gcagcatagc gcgacaacgc
    21721 ttccagcccc agcgcgccgc ttccggcgaa gcaatccaga cattgggcgt caacaatgac
    21781 cggagccagc cagttaaaca acgtttcgcg tacgcggtcg gtggtggggc gcagacctgg
    21841 gctatcagga accgggagtt tacggcctcg ccactgcccg ccaataatgc ggatttggcc
    21901 gctgccggaa tgattcggtt ttttcatgat aaattgctca atccgccaga taacacatac
    21961 ttgcaggcgg tgatgtgaat taagttaagt gatagactat ttcatcattt ttttagctgc
    22021 tatgtacata gcgttaacgc tgtgccatga agcaacagcg aggagtgtag tcgcaaatgg
    22081 cgaaagaaaa aaaacgtggc tttttttcct ggctgggctt tggtcaaaaa gagcagaccc
    22141 cggaaaaaga gacagaagtt cagaatgaac aaccggttgt agaagaaatc gttcaggcgc
    22201 aagagcctgt gaaggcttcg gaacaagccg ttgaagagca gccgcaggcg catactgaag
    22261 ccgaggcgga aacttttgct gccgacgttg tggaagtcac tgaacaggtt gctgaaagtg
    22321 aaaaagcgca gcctgaagcg gaagtcgttg cacagccgga accggtcgca gaagaaacgc
    22381 cggagccagt ggctatcgaa cgtgaagagc tgccgttgcc agaagacgtc aacgccgaag
    22441 aggtttcgcc agaagagtgg caggctgaag cggaaaccgt agagattgtc gaagcggcag
    22501 aagaagaagc ggctaaagaa gaaatcaccg acgaagagct ggaagcacag gcgctggctg
    22561 ccgaagcggc agaagaggcg gtgatggtag ttcctccggc agaagaagag cagccggttg
    22621 aagaaattgc ccaggagcaa gaaaaaccga ccaaagaagg tttcttcgcg cgcctgaaac
    22681 gcagcctgtt aaaaaccaaa gaaaatctcg gttccggatt tatcagcctg ttccgcggta
    22741 aaaaaatcga cgatgatctg tttgaggagc tagaagaaca gttgttgatc gccgatgtgg
    22801 gcgtggaaac cacacgtaaa attatcacca atctgacgga aggcgcatcc cgcaagcagc
    22861 ttcgtgatgc cgaggcgctc tatggcctgc tgaaagaaga gatgggcgag attctggcga
    22921 aagtcgatga gccgctgaat gttgaaggta aaacgccgtt cgtgatcctg atggtgggcg
    22981 tcaacggtgt gggtaaaact acgacgattg gtaagctggc acgtcagttt gagcagcagg
    23041 gtaaatcggt gatgctggcg gcgggcgata ctttccgtgc agcagcggtt gaacagcttc
    23101 aggtctgggg tcagcgcaac aatattccgg tgattgccca gcataccggt gcggattccg
    23161 cctctgttat cttcgacgcc attcaggcag ctaaagcgcg taatatcgac gtcctgattg
    23221 ccgataccgc cggacgcctg cagaacaaat cgcacctgat ggaagagttg aagaaaatcg
    23281 tccgcgtgat gaagaaactc gacgttgaag cgccgcatga agttatgctg actatcgatg
    23341 ccagtaccgg gcagaacgcg gtaagccagg ccaaactgtt ccatgaagct gttggcttaa
    23401 ccggcatcac gctaacgaaa ctggacggca cggcgaaagg cggggtaatt ttctcggtgg
    23461 ctgaccagtt tggtatccct atccgctaca ttggtgtcgg cgaacgtatt gaggatttgc
    23521 gtccgtttaa ggcggacgac tttatagagg cactttttgc ccgagaggat taacaatgat
    23581 tcgctttgaa catgtcagca aggcttatct cggtgggaga caggcgctgc agggcgttac
    23641 gttccatatg cagccgggtg agatggcgtt tctgaccggt cattccggcg cagggaaaag
    23701 taccctcctg aagctgatct gtgggattga gcggcccagc gccgggaaaa tctggtttag
    23761 cggccatgac atcacgcgtc tgaaaaaccg tgaagttccg tttctgcgcc gccagattgg
    23821 catgattttc caggatcacc atctactgat ggaccgtact gtctacgata acgtggcgat
    23881 cccgctgatt atcgctggtg ccagcggtga cgatattcgt cgccgggtgt cggcggcgct
    23941 ggataaagtc gggctactgg acaaagcgaa gaacttccct attcagcttt ccggcggtga
    24001 acaacagcgt gttggcattg cccgcgcggt ggtgaacaag cccgcggtac tgctggcgga
    24061 cgaaccgact ggtaacctgg acgacgcgtt gtcggaaggg attctacgtc tgtttgaaga
    24121 gtttaaccgc gttggggtaa ccgtattgat ggcaacgcac gacatcaacc tgatttcgcg
    24181 gcgttcctat cgcatgctca ccctgagcga tggtcacttg catggaggcg tgggccatga
    24241 ataagcgcga tgcaatcaat catattcggc agtttggcgg gcgtctggat cgcttccgta
    24301 aatcggtcgg cggctcaggc gacggcggtc gtaacgcacc gaaacgcgcg aaatcctcgc
    24361 caaaaccggt aaatcgcaaa accaacgttt tcaacgaaca ggtgcgctat gccttccacg
    24421 gcgcattgca ggatctgaaa agcaaaccgt tcgccacgtt tttaacggtg atggttatcg
    24481 ccatctccct gacgctgccc agcgtctgtt atatggtgta caaaaacgtt aatcaggcgg
    24541 cgacgcagta ttatccgtca ccgcaaatca ctgtttactt gcaaaaaacg ctggacgatg
    24601 acgccgctgc gggcgtggtg gcacagttgc aggccgagca aggcgtggag aaagtgaact
    24661 atctttctcg tgaagacgca ctgggcgagt tccgtaactg gtctggtttt ggtggtgcgc
    24721 tggatatgct ggaagaaaac ccgcttccgg cagtggcggt ggtgatcccg aaactcgatt
    24781 ttcaggggac ggaatcactc aatacgctgc gtgatcgtat cacccagatt aacggcattg
    24841 acgaagtgcg gatggatgac agctggtttg cccgtctggc ggcgttgacc gggctggtcg
    24901 ggcgcgtttc cgcgatgatc ggcgtgttga tggtggcagc agtgttcctc gtcatcggca
    24961 acagtgtgcg tctgagcatc tttgctcgcc gtgactccat taacgtgcag aaactgattg
    25021 gtgcgacgga tggtttcatc ctgcgcccgt tcctgtacgg aggcgcattg ctgggatttt
    25081 ctggcgcatt gctgtcatta attttgtcag aaattctggt actgcgtttg tcatcggcgg
    25141 ttgcagaagt ggcacaggtt ttcggaacga agtttgatat caatggttta tcgttcgatg
    25201 aatgcctgct attgctgctg gtatgctcga tgattggctg ggtggcagcg tggcttgcca
    25261 cggtacaaca tttacgccac tttacgcctg aataataaaa gcgtgttata ctctttccct
    25321 gcaatgggtt ccgtagcagg gaaagagatc ccgttgtctc ttcccggtat ttcatctcta
    25381 tgtcacattt tgtgcgtaat ttattcacaa gcttgcattg aacttgtgga taaaatcacg
    25441 gtctgataaa acagtgaatg ataacctcgt tgctcttaag ctctggcaca gttgttgcta
    25501 ccactgaagc gccagaagat atcgattgag aggatttgaa tgactgacaa aatgcaaagt
    25561 ttagctttag ccccagttgg caacctggat tcctacatcc gggcagctaa cgcgtggccg
    25621 atgttgtcgg ctgacgagga gcgggcgctg gctgaaaagc tgcattacca tggcgatctg
    25681 gaagcagcta aaacgctgat cctgtctcac ctgcggtttg ttgttcatat tgctcgtaat
    25741 tatgcgggct atggcctgcc acaggcggat ttgattcagg aaggtaacat cggcctgatg
    25801 aaagcagtgc gccgtttcaa cccggaagtg ggtgtgcgcc tggtctcctt cgccgttcac
    25861 tggatcaaag cagagatcca cgaatacgtt ctgcgtaact ggcgtatcgt caaagttgcg
    25921 accaccaaag cgcagcgcaa actgttcttc aacctgcgta aaactaagca gcgtctgggc
    25981 tggtttaacc aggatgaagt cgaaatggtg gcccgtgaac tgggcgtaac cagcaaagac
    26041 gttcgtgaga tggaatcacg tatggcggca caggacatga cctttgacct gtcttccgac
    26101 gacgattccg acagccagcc gatggcaccg gtgctctatc tgcaggataa atcatctaac
    26161 tttgccgacg gcatcgaaga tgataactgg gaagagcagg cggcaaaccg tctgaccgac
    26221 gcgatgcaag gtctggacga acgcagccag gacatcatcc gcgcgcgctg gctggacgaa
    26281 gacaacaagt ccacgttgca ggaactggct gaccgttacg gtgtttccgc tgaacgtgtg
    26341 cgccaactgg aaaagaacgc gatgaaaaaa cttcgcgccg ctattgaagc gtaatttccg
    26401 ctattaagca gagaaccctg gatgagagtc cggggttttt gttttttggg cctctacaat
    26461 aatcaattcc ccctccggca aaacgtcaat ccccacgcag attgttaata aactgtcaaa
    26521 atagctattc caatatcata aaaatggggt atgttttagc agagtatgct gctaaagcac
    26581 gggtagtcat gcataaaacg aaataaagtg ctgaatcaca acaccacaac acacgtaata
    26641 accagaagaa tggggattct caggatgaac ataaagggta aagcgttact ggcaggatgt
    26701 atcgcgctgg cattcagcaa tatggctctg gcagaagata ttaaagtcgc ggtcgtgggc
    26761 gcaatgtccg gtccggttgc gcagtacggt gaccaggagt ttaccggcgc agagcaggcg
    26821 gttgcggata tcaacgctaa aggcggcatt aaaggcaaca aactgcaaat cgtaaaatat
    26881 gacgatgcct gtgacccgaa acaggcggtt gcggtggcga acaaagtcgt taacgacggc
    26941 attaaatatg tgattggtca cctctgttct tcatcaacgc agcctgcgtc tgacatctac
    27001 gaagacgaag gcattttaat gatcacccca gcggcaaccg cgccggagct gaccgcccgt
    27061 ggctatcagc tgatcctgcg caccaccggc ctggactccg accaggggcc gacggcggcg
    27121 aaatatattc ttgagaaagt gaaaccgcag cgtattgcta tcgttcacga caaacagcaa
    27181 tacggcgaag gtctggcgcg agcggtgcag gacggcctga agaaaggcaa tgctaacgtg
    27241 gtgttctttg atggtatcac cgccggggaa aaagatttct caacgctggt ggcccgtctg
    27301 aaaaaagaga atatcgactt cgtttactac ggcggttatc acccggaaat ggggcaaatc
    27361 ctgcgtcagg cacgcgcggc agggctgaaa actcagttta tggggccgga aggtgtggct
    27421 aacgtttcgc tgtctaacat tgcgggcgaa tcagcggaag ggctgctggt gaccaagccg
    27481 aagaactacg atcaggttcc ggcgaacaaa cccattgttg acgccatcaa agcgaaaaaa
    27541 caggacccaa gcggcgcatt cgtttggacc acctacgccg cgctgcaatc tttgcaggcg
    27601 ggcctgaatc agtctgacga tccggctgag atcgccaaat acctgaaagc gaactccgtg
    27661 gataccgtga tgggtccgct gacgtgggat gagaaaggtg atctgaaagg ctttgagttc
    27721 ggcgtatttg actggcacgc caacggcacg gcgaccgatg cgaagtaatc attaatcggc
    27781 aactttgggt tgccgccaaa ttgctaatat cgagtacgtt gcttcatgcc ggatgcggcg
    27841 taaacgcctt atccggccta caagatccaa agaaatcagt aaattgcaac acacattgta
    27901 ggcctgataa gcgtagcgca tcaggcaatt tctcgttggt catcactttt gaaatcaact
    27961 tgacgattaa cgcttctccc agccgccctg ttgcgccgta aaccccagcg cctgcataaa
    28021 cgccgtcatc acaccgcgat cttccacgcc tgcatccgcc atccaccagc atgaaacggc
    28081 aggattgtta cgcaaaacct cttccagcag atattgcccc acaccgcgac ggcgggtgac
    28141 ttcccgcacg cgcagggaat ccagtgctcc ctcggtgccg cttaaggtta cccgcacggc
    28201 cgctagcagg cgctcgttaa aacgcgcggc gtagatgcgg tggttatcgt caacctgtaa
    28261 agaggaaggg gaatactccg gccagatctt ttgcaggtca atccggtcct ggtcgctaaa
    28321 gttttctaat cgaatgatgg tcagcttcat ggggaacccg tgtaaatcac aaaagtggaa
    28381 ccagtgtagc gaaataattt aatcggaggc tttctctttt ttatttcttt tggcaggtga
    28441 ttaatttttt aacagcaata attacaaagt taaaacatta gagaatgaaa aatacccagc
    28501 ataatcccct gaatggtagt gaattattcc gctctttgta ccgttatttt atgctgacaa
    28561 cggcgctttt ttctgtttat ctatcaataa attcagaata ttatctgttc ttaatcgact
    28621 gaaaaatagg gattttaatc gctattatca caaaatactg cgctaaaccc ttaatcagac
    28681 aggcaaaaac agtgcagtat aaaaaaagaa cagtctgatt tgttaacaca taaaaacaaa
    28741 gcaacacaac atcacgaatg gggatttttg actatgaaac ggaatgcgaa aactatcatc
    28801 gcagggatga ttgcactggc aatttcacac accgctatgg ctgacgatat taaagttgcc
    28861 gttgtcggcg cgatgtccgg cccgattgcc cagtggggcg atatggaatt taacggcgcg
    28921 cgtcaggcga ttaaagacat taatgccaaa gggggaatta aaggcgataa gctggtgggc
    28981 gtggaatatg acgacgcctg cgacccgaaa caagccgttg cggtcgccaa caaaatcgtt
    29041 aacgacggca ttaaatacgt tattggtcat ctgtgttctt cttctaccca acctgcgtca
    29101 gatatctacg aagacgaagg tattctgatg atctcgccgg gagcgaccaa cccggagctg
    29161 acccaacgcg gttatcaaca cattatgcgt actgccgggc tggactcttc ccaggggcct
    29221 acggcggcaa aatacattct tgagacggtg aagccccagc gcatcgccat cattcacgac
    29281 aaacaacagt atggcgaagg gctggcgcgt tcggtgcagg acgggctgaa agcggctaac
    29341 gccaacgtcg tcttcttcga cggtattacc gccggggaga aagatttctc cgcgctgatc
    29401 gcccgcctga aaaaagaaaa catcgacttc gtttactacg gcggttacta cccggaaatg
    29461 gggcagatgc tgcgccaggc ccgttccgtt ggcctgaaaa ctcagtttat ggggccggaa
    29521 ggtgtgggta acgcatcatt gtcgaatatt gctggcgatg ctgccgaagg catgttggtc
    29581 actatgccaa aacgctatga ccaggatccg gcaaaccagg gcatcgttga tgcgctgaaa
    29641 gcagacaaga aagatccgtc cgggccttat gtctggatca cctacgcggc ggtgcaatct
    29701 ctggcgactg cccttgagcg tactggcagc gatgagccgc tggcgctggt gaaagattta
    29761 aaagctaacg gtgcaaacac cgtgattggg ccgctgaact gggatgaaaa aggcgatctt
    29821 aagggatttg attttggtgt cttccagtgg cacgccgacg gttcatccac ggcagccaag
    29881 tgaaaatccc accgcccgtg aaaaacgggc gggtttagaa aggttacctt atgtctgagc
    29941 agtttttata tttcttgcag cagatgttta acggcgtcac gctgggcagt acctacgcgc
    30001 tgattgccat cggctacacc atggtttacg gcattatcgg catgatcaac ttcgcccacg
    30061 gcgaggttta tatgatcggc agctatgtct catttatgat catcgccgcg ctgataatga
    30121 tgggcattga taccggctgg ctgctggtgg cggcgggatt cgtcggcgca atcgtcatcg
    30181 ccagcgccta cggctggagt atcgaacggg tagcctatcg cccggtgcgt aactctaagc
    30241 gcctgattgc gctcatctcg gcaatcggca tgtccatctt cctgcaaaac tacgttagcc
    30301 tgactgaagg ttcgcgtgac gtggcgctgc ccagtctgtt taacggtcag tgggtggtgg
    30361 ggcatagcga aaacttctct gcctctatta ccaccatgca ggcggtgatc tggattgtca
    30421 ccttccttgc catgctggcg ctgacggttt tcattcgcta ttcccgtatg gggcgcgcgt
    30481 gtcgtgcctg cgcggaagac ctgaaaatgg cgagcctgct tggcattaac accgaccggg
    30541 tgattgcgct gacctttgtg attggcgcgg cgatggcggc ggtggcaggt gtgctgctcg
    30601 gtcagttcta cggcgtaatt aacccctaca tcggctttat ggccgggatg aaagccttta
    30661 ccgctgcggt gctcggtggg attggcagca ttccgggagc gatgattggc gggctgattc
    30721 tggggattgc ggaggcgctc tcttctgcct atctgagtac ggaatataaa gatgtggtct
    30781 cattcgccct gctgattctg gtgctgctgg tgatgccgac cggtattctg ggtcgcccgg
    30841 aggtagagaa agtatgaaac cgatgcatat tgcaatggcg ctgctctctg ccgcgatgtt
    30901 ttttgtccta gcgggcgtct ttatgggcgt gcaactggag ctggatggca ccaaactggt
    30961 ggtcgacacg gcttcagaca tccgttggca gtgggtgttt atcggcacgg cggtggtctt
    31021 tttcttccag cttttgcgac cggctttcca gaaagggcta aaaagcgttt ccggaccgaa
    31081 gtttattctg cccgcgattg atggctccac ggtgaagcag aaactattcc tcgtggcgct
    31141 gctggtgctt gcggtggcgt ggccgtttat ggtgtcgcgt gggacggtgg atatcgccac
    31201 cctgaccatg atctacatta tcctcggcct cgggctgaac gtggttgttg gtctttctgg
    31261 tctgctggtg ctggggtacg gcggctttta cgccatcggc gcttacactt ttgcgctgct
    31321 caatcactat tacggcctgg gcttctggac ctgtctgccg attgccgggt taatggcagc
    31381 ggcggccggc ttcctgctcg gcttcccggt gcttcgcttg cgcggcgact atctggcgat
    31441 cgttaccctc ggtttcggcg aaattgtgcg catattgctg ctcaataaca ccgaaattac
    31501 cggcggcccg aacggaatca gtcagatccc gaaaccgaca ctcttcggac tcgagttcag
    31561 ccgtaccgct cgtgaaggcg gctgggacac gttcagtaat ttctttggcc tgaaatacga
    31621 tccctccgat cgtgtcatct tcctctacct ggtggcgttg ctgctggtgg tgctaagcct
    31681 gtttgtcatt aaccgcctgc tgcggatgcc gctggggcgt gcgtgggaag cgttgcgtga
    31741 agacgagatc gcctgccgtt cgctgggctt aagcccgcgt cgtatcaagc tgactgcctt
    31801 taccataagt gccgcgtttg ccggttttgc cggaacgctg tttgctgcgc gccagggctt
    31861 tgtcagcccg gaatccttca cctttgctga atcggcgttt gtactggcga tagtggtgct
    31921 cggcggtatg ggatcgcagt ttgcggtgat tctggcggca attttgctgg tggtatcgcg
    31981 cgaattgatg cgtgatttca acgaatacag catgttaatg ctcggtggtt tgatggtgct
    32041 gatgatgatc tggcgtccgc agggcttgct gcccatgacg cgcccgcaac tgaagctgaa
    32101 aaacggcgca gcgaaaggag agcaggcatg agtcagccat tattatctgt taacggtctg
    32161 atgatgcgct tcggcggctt gctggcggtg aacaacgtca atcttgaact gtacccgcag
    32221 gagatcgtct cgttaatcgg ccctaacggt gccggaaaaa ccacggtttt taactgcctg
    32281 accggattct acaaacccac cggcggcact attttactgc gtgatcagca tctggaaggt
    32341 ttaccggggc agcaaattgc ccgcatgggc gtggtgcgta ccttccagca tgtgcgtctg
    32401 ttccgtgaaa tgacggtgat tgaaaacctg ctggtggcgc agcatcaaca actgaaaacc
    32461 gggctgttct ctggcctgtt gaaaacgcct tctttccgtc gcgcccagag cgaagcgctc
    32521 gaccgcgccg ccacctggct tgagcgcatt ggtttgctgg aacacgccaa ccgtcaggcg
    32581 agtaacctgg cctatggtga ccaacgccgt ctggagattg cccgctgcat ggtgacgcag
    32641 ccggagattt taatgctcga cgaaccggcg gcaggtctta acccaaaaga aaccaaagag
    32701 ctggatgagc tgattgccga actgcgcaat catcacaaca ccactatctt gttgattgaa
    32761 cacgatatga agctggtgat ggggatttca gaccgaattt atgtggtcaa tcaggggacg
    32821 ccactggcaa acggtacgcc ggagcaaatc cgtaataacc cggacgtgat ccgtgcctat
    32881 ttaggtgagg cataagatgg aaaaagtcat gttgtccttt gacaaagtca gcgcccacta
    32941 cggcaaaatc caggcgctgc atgaggtgag cttgcatatc aatcagggcg agattgtcac
    33001 gctgattggc gcgaacgggg cggggaaaac caccttgctt ggtacgttat gcggcgatcc
    33061 gcgcgccacc agcgggcgaa ttgtgtttga tgataaagac attaccgact ggcagacggc
    33121 gaaaatcatg cgcgaagcgg tagcgattgt cccggaaggg cgtcgcgtct tctcgcggat
    33181 gacggtggaa gagaacctgg cgatgggcgg cttctttgcc gaacgtgacc agtttcagga
    33241 gcgcataaag tgggtgtatg agctgtttcc acgtcttcat gagcgtcgtg ttcagcgggc
    33301 gggcaccatg tccggcggtg aacagcagat gctggcgatt ggtcgcgcgc tgatgagcaa
    33361 cccgcgtttg ttgttgcttg atgagccatc gctcggtctt gcgccgatta tcatccagca
    33421 aattttcgac accatcgagc agctgcgcga gcaggggatg actatcttcc tcgtcgagca
    33481 gaacgccaac caggcgctaa agctggcgga tcgcggctac gtgctggaaa acggccatgt
    33541 ggtgctctcc gataccggcg acgcgctgct ggcgaacgaa gcagtgagaa gtgcgtattt
    33601 aggcgggcaa taacacgttg attgataagg agtcaaaaga ctcctttgag acaggtgaca
    33661 aatgtaaaat tgcctgatgc gctgcgctta tcaggcctac tgggtgagtt gcaatatatt
    33721 gaatttgcac gatcttgtag gccggataag gcgtttacgc cgcatccggc atgaaacgat
    33781 gagtaatctg tagagtttga ttcagacctt ctattttccg cttatccgtg ccccatctcc
    33841 cattttccct cacccacgcc gtcaccgcct tgtcatcttt ctgacacctt actatcttac
    33901 aaatgtaaca aaaatgttat ttttctgtaa ttcgagcatg tcatgttacc ccgcgagcat
    33961 aaaacgcgtg aattcgcgca ttcggtacaa caagagagat aaacgatgaa accgttacat
    34021 tatacagctt cagcactggc gctcggactg gcgttaatgg ggaatgcaca ggcagtgacg
    34081 accattccgt tctggcattc tatggaaggg gaactgggta aagaggtgga ttctctggcc
    34141 caacgtttta acgccgaaaa tccggattac aaaattgtac cgacctataa aggcaactac
    34201 gaacagaatt taagcgcggg gattgccgca tttcgtaccg gcaacgctcc ggctattttg
    34261 caggtttatg aagttggcac cgccaccatg atggcatcga aagccattaa accggtatat
    34321 gacgtgttta aagaggcggg gattcaattc gatgagtcgc agtttgtgcc gacggtttcc
    34381 ggttactact ccgacagcaa aacgggccac ttactctccc agccattcaa cagctcgacc
    34441 cccgttctct attacaacaa agacgccttc aagaaagcag gattagaccc ggaacaaccg
    34501 ccgaaaacct ggcaggatct ggcggactat gccgcgaaac tgaaagcctc cggtatgaag
    34561 tgcggctacg ccagcggctg gcagggctgg atccaactgg aaaactttag cgcctggaac
    34621 ggtctgccgt ttgccagcaa aaacaacggc tttgacggca cggacgcggt gctggagttc
    34681 aacaagccgg agcaggtgaa acacatcgcc atgctcgagg agatgaacaa gaagggcgac
    34741 ttcagctacg tcggtcgtaa ggatgaatcc accgagaagt tctataacgg tgattgcgcg
    34801 atgaccaccg cctcttccgg ttctctcgct aatattcgtg agtacgccaa atttaactat
    34861 ggcgtaggca tgatgcctta cgacgccgat gcgaaagatg caccgcaaaa cgccattatc
    34921 ggcggggcca gcctgtgggt gatgcagggc aaaaataaag aaacgtatac cggcgtggcg
    34981 aagttcctcg acttcctcgc gaagccagaa aacgctgccg agtggcatca gaaaaccggt
    35041 tatctgccaa tcaccaaagc agcgtatgac ctgacccgtg agcagggctt ttatgagaaa
    35101 aacccagggg cggataccgc gacgcgtcag atgctgaata agccgccgtt gccgttcacc
    35161 aaagggctgc gtctgggcaa catgccgcag atccgcgtga ttgtggatga agagctggag
    35221 agcgtgtgga ccggtaagaa gacaccacag caggcactgg ataccgccgt tgagcgtgga
    35281 aatcagttgc tgcgccgctt tgagaaatcg acgaagtctt aatcagtgta atgtcggatg
    35341 cgtttcgctt atctgacctg gcatcgcgtg taggccggat aagcgaagcg catccggcac
    35401 agttcaggaa ttaaccgtaa tgtcatcatc ccgtccggtg ttccgctcgc gctggctgcc
    35461 ttatctgctg gtcgcgccgc agctcatcat caccgttatc ttttttatct ggcctgcggg
    35521 cgaagcgttg tggtactcgc tacaaagcgt cgatccgttt ggtttctcca gccagtttgt
    35581 cggcctggat aacttcgtcg cgctgtttca tgacagctac tatctcgact ccttctggac
    35641 gacgataaaa ttcagcacct ttgtcaccgt cagcggtttg ctggtgtcgc tgttcttcgc
    35701 ggcgctggtg gagtacatcg tgcgcggcag ccgtttctat caaaccttaa tgttgctgcc
    35761 ttacgccgtg gctcccgccg ttgccgccgt attgtggatc ttcctgttta accccggtcg
    35821 cgggctgatc acccattttc tcgcggagtt cggctacgac tggaaccacg cgcaaaacag
    35881 cggtcaggca atgtttctgg tggtgtttgc ctcagtatgg aagcaaatca gctacaactt
    35941 cctgttcttc tatgccgcgc tgcaatccat tccccgttcg ttgatcgaag ccgcagccat
    36001 cgacggtgca gggccgattc gccgcttctt taagattgcg ctgccgctta tcgccccggt
    36061 gagtttcttc ctgctggtag tgaacctggt gtatgccttc ttcgacacct tcccggtgat
    36121 cgacgccgcc acgtccggcg ggccggttca ggccaccacg acgctgattt ataagatcta
    36181 ccgcgaaggt tttaccggac tggatctggc ttcgtctgcc gcacagtcgg tggtgttgat
    36241 gttcctcgtc atcgtgctga cggtggtgca gttccgctat gttgaaagca aggtgcgtta
    36301 ccaatgattg agaaccgtcc gtggctgacg atattcagcc ataccatgct gatcctcggg
    36361 atcgcggtga tcctcttccc gctgtacgtg gcgtttgtcg cggcgacgct ggataaacag
    36421 gccgtctatg ccgcgccgat gacgctcatc cccggcacac atctgctgga aaacatccac
    36481 aacatctggg tgaacggggt aggcacgaat agcgcgccgt tctggcggat gttgcttaac
    36541 agctttgtga tggcgttcag cattacgctc ggcaaaatta ccgtctcgat gctctcggca
    36601 tttgccattg tctggtttcg ttttccgcta cgtaacctct tcttctggat gatttttatc
    36661 accctgatgc tgccggttga agtacgtatc ttcccgacgg tggaagtcat cgccaacctg
    36721 cagatgctcg acagctacgc cggtttaacg ctgccgctga tggcctcggc gaccgctact
    36781 ttcctgttcc gccagttctt tatgacgctg ccggatgagc tggtggaagc cgcgcggatc
    36841 gacggcgcat cgccaatgcg cttcttttgc gacatcgttt ttccgctctc caaaactaat
    36901 ctggcggcgc tgtttgtgat cacctttatc tacggctgga atcagtattt gtggccgttg
    36961 ttgattatta ccgacgttga tctcggcacc accgtggcag ggatcaaagg gatgatcgcc
    37021 actggcgaag gcaccacgga atggaactcg gtaatggcgg cgatgttgtt aacgcttatc
    37081 cctccggtgg tgattgtttt agtgatgcag cgtgccttcg tgcgcggcct ggtcgatagt
    37141 gagaaataag atggcaggac tgaaattaca ggcagtaacc aaaagctggg atggcaaaac
    37201 ccaggtgatt aaaccactga cccttgatgt ggcggatggc gaatttatcg tgatggtcgg
    37261 gccgtctggc tgcggaaaat cgacgctgct gcgcatggtt gccgggctgg agcgggtgac
    37321 ggaaggcgat atctggatca acgaccagcg cgtgaccgaa atggagccga aagatcgcgg
    37381 gattgcgatg gtgttccaga actacgcgct ttatccgcat atgagcgtcg aagaaaacat
    37441 ggcgtggggg ctgaaaattc gcggcatggg caagcagcaa atagccgagc gcgttaaaga
    37501 agcggcgcgc attctggagc tggatggcct gcttaagcgc cgcccgcgcg agctatccgg
    37561 cggtcagcgt cagcgtgtgg cgatgggtcg cgcgattgtg cgcgatccgg cggtgttcct
    37621 gtttgatgag ccgctctcta acctcgatgc caagctgcgc gtgcagatgc gccttgagct
    37681 gcaacagctg caccgtcgcc tgaaaacgac ttcactctac gttactcacg atcaggttga
    37741 agcgatgacg ctcgcccagc gagtaatggt gatgaacggc ggcgttgccg aacagattgg
    37801 cacaccagtt gaagtctacg aaaagcccgc cagcctgttt gtagcgagtt ttatcggcag
    37861 tccggcgatg aacctgctga caggccgcgt gaataacgaa ggcacgcatt tcgaactgga
    37921 cggcggtatt gagctgccgc taaacggtgg ctaccgtcag tatgccgggc gtaaaatgac
    37981 tctcggcatt cgcccggaac atattgcgct aagctcgcag gcagaaggcg gcgtaccgct
    38041 ggtgatggac acgctggaga tcctcggcgc agataacctg gcgcacggac gctggggcga
    38101 gcagaagctg gtggtgcgac tggcgcatca ggagcgcccg acggcaggca gcacgctgtg
    38161 gctgcatctg gcggaaaatc agctgcatct ttttgatggt gaaacaggac aacgagtatg
    38221 agtaactggc cttatccccg catcgtcgct catcgtggcg gcggtaagct ggccccggaa
    38281 aacaccctgg cggcaatcga cgtcggggca aaatacggtc ataagatgat cgaatttgac
    38341 gcgaagttat cgaaagatgg cgagatcttc ctgctccatg acgacaatct cgaacgcacc
    38401 agcaacggct ggggcgtcgc gggtgaactg aactggcagg atttactgcg cgtggatgcg
    38461 ggcagttggt acagcaaagc gtttaaaggt gagtcgctgc cgttgctttc gcaggtggcg
    38521 gaacgctgtc gcgaacacgg gatgatggcg aatatcgaaa tcaaacccac cactggcact
    38581 ggaccattaa cgggcaaaat ggtggcgctg gcggcacgcc aactgtgggc cggtatgacg
    38641 ccgccgctgc tgtcatcgtt tgagattgat gctttagaag ctgcacagct ggcggcaccg
    38701 gaactgccgc gtggtttatt gcttgatgag tggcgcgacg actggcgcga actgaccgcg
    38761 cggctgggct gcgtctctat tcatctcaat cataagttac tcgataaagt gcgagtgatg
    38821 cagttgaaag acgccggact gcggattctg gtttataccg tcaataaacc ccagcgcgcg
    38881 gcagagttgc tgcgctgggg cgtggattgc atctgtaccg atgcgattga cgtgattggt
    38941 ccgaacttta cggcccaata gttttcaacg gaatgtcagg ctgcggcgtg ctgggctgat
    39001 tcagcatgtc gccgtttctc ctctccggca gcatatgctg ctgattcaac gaactatccg
    39061 gattacggtt gctgtttaac atcccgccgt tggtgttggg cagcatttgc tgccgggcgg
    39121 gatttcgctc ccccggctgt gactgcaaca cccgctgaga attattgttt atctggtttt
    39181 ctaaatgctg ctgttgcaac tgagtttgcg ttttcagttg ctgattcagc atcccttttt
    39241 gctggatttg ctgagtctgc atctgggttt gcatccgctg ctggctgggt atctgatacc
    39301 ccggctggtt agggttgttc agagtattaa tgggctgtgc aaagccgaca aacggcagga
    39361 gtgccgtaag aatcagaagt cgtttcatcg cgtatcctcc tctgaagata tcctttaagt
    39421 ttactcgctt cccgacaaaa cgatgattaa ttcagagtta tataccaggc ttagctgggg
    39481 ttgcccctta atctctggag aataacgatg ataaaaccga cgtttttacg ccgggtggcc
    39541 attgctgctc tgctctcagg aagttgtttt agcgccgccg ccgcgcctcc tgcgccgccc
    39601 gtctcgtatg gtgtggagga agatgtcttc cacccggtac gcgcgaaaca gggaatggta
    39661 gcgtctgtgg acgccactgc cactcaggtg ggggtggata ttctcaagga gggcgggaat
    39721 gccgttgatg ccgccgtggc ggtgggctac gcgctggcgg taacgcatcc gcaggcaggg
    39781 aatctgggcg gtggtggttt tatgctgatc cgctcgaaaa atggcaatac caccgctatc
    39841 gatttccgcg aaatggcacc cgctaaagcg acccgcgata tgttccttga tgaccagggc
    39901 aacccggaca gcaaaaaatc actcacttcg catctggctt ccggcacacc aggtacggtc
    39961 gctggtttct cgctggcgtt ggataagtac ggcactatgc cgttgaacaa agtggtgcag
    40021 cccgcgttca aactggcacg cgatggtttt atcgttaacg atgctttggc agacgatctc
    40081 aaaacctacg gtagcgaagt gttgccgaat cacgaaaaca gcaaagctat cttctggaaa
    40141 gagggcgaac cgctgaaaaa gggcgacacg ctggtgcagg cgaacctggc aaagagcctg
    40201 gagatgattg ctgaaaacgg cccggacgaa ttctataaag gcacgattgc ggaacagatc
    40261 gcccaggaga tgcagaaaaa cggtggcttg atcactaaag aagatttagc ggcctataaa
    40321 gcggtcgaac gcactccgat aagcggcgat tatcgcgggt atcaggttta ctccatgcca
    40381 ccgccatcct ccggcgggat ccatatcgta caaatcctca atattctgga aaacttcgat
    40441 atgaagaaat acggctttgg cagcgccgat gcgatgcaaa tcatggcaga agcggagaaa
    40501 tacgcctacg ccgaccgctc ggaatatctt ggcgacccgg attttgtcaa agtcccgtgg
    40561 caggcactga ccaataaagc ctatgccaaa tccattgccg atcaaattga tatcaacaaa
    40621 gcgaagccgt ccagtgagat tcgccctggc aagcttgcgc cttatgagag taatcaaact
    40681 acccattact ctgtagtgga taaagacggt aacgcggtgg cggtgaccta tacgctgaac
    40741 accaccttcg gtacgggtat tgtcgcgggc gagagcggta ttctgcttaa taaccagatg
    40801 gatgatttct ccgccaaacc gggcgtaccg aacgtttacg ggctggtggg cggtgatgcc
    40861 aacgccgtcg ggccgaacaa acgcccgctg tcgtcgatgt cgccgaccat tgtggtgaaa
    40921 gacggtaaaa cctggctggt taccggtagc ccaggcggta gccggatcat cactacagtg
    40981 ctgcaaatgg tggtgaatag catcgattat ggcatgaacg tcgccgaagc gaccaatgcg
    41041 ccgcgtttcc accatcagtg gttgccggac gagctgcgtg tcgaaaaagg gtttagcccg
    41101 gatacgctca agctgctgga agcaaaaggt cagaaagtgg cgctgaaaga ggcgatgggc
    41161 agtacacaaa gcattatggt tgggccggac ggtgagttgt acggcgcatc cgacccgcgc
    41221 tcggtggatg atttaacggc ggggtactaa ggttagcggc cctcttcgtg ggaagagggc
    41281 tattttgtca gggcaagccg aaggtagcct tctttatttc gtaatcctgt agatattctt
    41341 ccagcggctt tgccgttccc agagaatgaa tttcaccact gtctttatca ataataaaag
    41401 gtgcgttacc agctaagcgc gcggcctcat ctccagtttc gagaaattct cgtgcttcga
    41461 aacagaaata ccagccctgg ctaaagcgtc catgtagagt aatgacgacc gggagatctg
    41521 catcatcaag gtaatggttc gctttcgcga atgcgtcgtg ataagtaatc ataattataa
    41581 taaatattat tgttgagtgt tatattatta ttcctgtgtg atacattgag caaagacgcg
    41641 ttcatcttcg tcaaagatat tattaaatgc tggttttgaa aaaagttcat cggataagat
    41701 atagtcacga aataagtatc tgctaatatt ttatgatttc tttttcagaa cgtcgaacgg
    41761 gattttctta ttt
//
LOCUS       DAZGAN020000031        41755 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGAN020000031 DAZGAN020000000
VERSION     DAZGAN020000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 41755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.031"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(99..1352)
                     /gene="amiC"
                     /locus_tag="ACHKYO_RS09465"
                     /old_locus_tag="pgaptmp_001893"
     CDS             complement(99..1352)
                     /gene="amiC"
                     /locus_tag="ACHKYO_RS09465"
                     /old_locus_tag="pgaptmp_001893"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HGU4428750.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1584..2915
                     /gene="argA"
                     /locus_tag="ACHKYO_RS09470"
                     /old_locus_tag="pgaptmp_001894"
     CDS             1584..2915
                     /gene="argA"
                     /locus_tag="ACHKYO_RS09470"
                     /old_locus_tag="pgaptmp_001894"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HGU4428751.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2977..4803)
                     /gene="recD"
                     /locus_tag="ACHKYO_RS09475"
                     /old_locus_tag="pgaptmp_001895"
     CDS             complement(2977..4803)
                     /gene="recD"
                     /locus_tag="ACHKYO_RS09475"
                     /old_locus_tag="pgaptmp_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HGU4428752.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4803..8345)
                     /gene="recB"
                     /locus_tag="ACHKYO_RS09480"
                     /old_locus_tag="pgaptmp_001896"
     CDS             complement(4803..8345)
                     /gene="recB"
                     /locus_tag="ACHKYO_RS09480"
                     /old_locus_tag="pgaptmp_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HGU4428753.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8338..11226)
                     /gene="ptrA"
                     /locus_tag="ACHKYO_RS09485"
                     /old_locus_tag="pgaptmp_001897"
     CDS             complement(8338..11226)
                     /gene="ptrA"
                     /locus_tag="ACHKYO_RS09485"
                     /old_locus_tag="pgaptmp_001897"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HGU4428754.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11402..14770)
                     /gene="recC"
                     /locus_tag="ACHKYO_RS09490"
                     /old_locus_tag="pgaptmp_001898"
     CDS             complement(11402..14770)
                     /gene="recC"
                     /locus_tag="ACHKYO_RS09490"
                     /old_locus_tag="pgaptmp_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HGU4428755.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLDSSQELDAFVDVMPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14783..15106)
                     /gene="ppdC"
                     /locus_tag="ACHKYO_RS09495"
                     /old_locus_tag="pgaptmp_001899"
     CDS             complement(14783..15106)
                     /gene="ppdC"
                     /locus_tag="ACHKYO_RS09495"
                     /old_locus_tag="pgaptmp_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGU4428756.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15091..15498)
                     /gene="ygdB"
                     /locus_tag="ACHKYO_RS09500"
                     /old_locus_tag="pgaptmp_001900"
     CDS             complement(15091..15498)
                     /gene="ygdB"
                     /locus_tag="ACHKYO_RS09500"
                     /old_locus_tag="pgaptmp_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HGU4428757.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIADYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15495..16058)
                     /gene="ppdB"
                     /locus_tag="ACHKYO_RS09505"
                     /old_locus_tag="pgaptmp_001901"
     CDS             complement(15495..16058)
                     /gene="ppdB"
                     /locus_tag="ACHKYO_RS09505"
                     /old_locus_tag="pgaptmp_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU4428758.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVHQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(16049..16519)
                     /gene="ppdA"
                     /locus_tag="ACHKYO_RS09510"
                     /old_locus_tag="pgaptmp_001902"
     CDS             complement(16049..16519)
                     /gene="ppdA"
                     /locus_tag="ACHKYO_RS09510"
                     /old_locus_tag="pgaptmp_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU4428759.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16703..17497)
                     /gene="thyA"
                     /locus_tag="ACHKYO_RS09515"
                     /old_locus_tag="pgaptmp_001903"
     CDS             complement(16703..17497)
                     /gene="thyA"
                     /locus_tag="ACHKYO_RS09515"
                     /old_locus_tag="pgaptmp_001903"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGU4428760.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17504..18379)
                     /gene="lgt"
                     /locus_tag="ACHKYO_RS09520"
                     /old_locus_tag="pgaptmp_001904"
     CDS             complement(17504..18379)
                     /gene="lgt"
                     /locus_tag="ACHKYO_RS09520"
                     /old_locus_tag="pgaptmp_001904"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGU4428761.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18530..20776)
                     /gene="ptsP"
                     /locus_tag="ACHKYO_RS09525"
                     /old_locus_tag="pgaptmp_001905"
     CDS             complement(18530..20776)
                     /gene="ptsP"
                     /locus_tag="ACHKYO_RS09525"
                     /old_locus_tag="pgaptmp_001905"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4428762.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20789..21319)
                     /gene="rppH"
                     /locus_tag="ACHKYO_RS09530"
                     /old_locus_tag="pgaptmp_001906"
     CDS             complement(20789..21319)
                     /gene="rppH"
                     /locus_tag="ACHKYO_RS09530"
                     /old_locus_tag="pgaptmp_001906"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HGU4428763.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(21672..21818)
                     /gene="ygdT"
                     /locus_tag="ACHKYO_RS09535"
                     /old_locus_tag="pgaptmp_001907"
     CDS             complement(21672..21818)
                     /gene="ygdT"
                     /locus_tag="ACHKYO_RS09535"
                     /old_locus_tag="pgaptmp_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HGU4428764.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22004..22693
                     /gene="mutH"
                     /locus_tag="ACHKYO_RS09540"
                     /old_locus_tag="pgaptmp_001908"
     CDS             22004..22693
                     /gene="mutH"
                     /locus_tag="ACHKYO_RS09540"
                     /old_locus_tag="pgaptmp_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HGU4428765.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22762..23475
                     /gene="ygdQ"
                     /locus_tag="ACHKYO_RS09545"
                     /old_locus_tag="pgaptmp_001909"
     CDS             22762..23475
                     /gene="ygdQ"
                     /locus_tag="ACHKYO_RS09545"
                     /old_locus_tag="pgaptmp_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU4428766.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23613..23831
                     /gene="ygdR"
                     /locus_tag="ACHKYO_RS09550"
                     /old_locus_tag="pgaptmp_001910"
     CDS             23613..23831
                     /gene="ygdR"
                     /locus_tag="ACHKYO_RS09550"
                     /old_locus_tag="pgaptmp_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HGU4428767.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23939..24979
                     /gene="tas"
                     /locus_tag="ACHKYO_RS09555"
                     /old_locus_tag="pgaptmp_001911"
     CDS             23939..24979
                     /gene="tas"
                     /locus_tag="ACHKYO_RS09555"
                     /old_locus_tag="pgaptmp_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HGU4428768.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25011..26204)
                     /gene="lplT"
                     /locus_tag="ACHKYO_RS09560"
                     /old_locus_tag="pgaptmp_001912"
     CDS             complement(25011..26204)
                     /gene="lplT"
                     /locus_tag="ACHKYO_RS09560"
                     /old_locus_tag="pgaptmp_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HGU4428769.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26197..28356)
                     /gene="aas"
                     /locus_tag="ACHKYO_RS09565"
                     /old_locus_tag="pgaptmp_001913"
     CDS             complement(26197..28356)
                     /gene="aas"
                     /locus_tag="ACHKYO_RS09565"
                     /old_locus_tag="pgaptmp_001913"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HGU4428770.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRMPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28942..29973
                     /gene="galR"
                     /locus_tag="ACHKYO_RS09570"
                     /old_locus_tag="pgaptmp_001914"
     CDS             28942..29973
                     /gene="galR"
                     /locus_tag="ACHKYO_RS09570"
                     /old_locus_tag="pgaptmp_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HGU4428771.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29980..31242)
                     /gene="lysA"
                     /locus_tag="ACHKYO_RS09575"
                     /old_locus_tag="pgaptmp_001915"
     CDS             complement(29980..31242)
                     /gene="lysA"
                     /locus_tag="ACHKYO_RS09575"
                     /old_locus_tag="pgaptmp_001915"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGU4428772.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDVGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31364..32299
                     /gene="lysR"
                     /locus_tag="ACHKYO_RS09580"
                     /old_locus_tag="pgaptmp_001916"
     CDS             31364..32299
                     /gene="lysR"
                     /locus_tag="ACHKYO_RS09580"
                     /old_locus_tag="pgaptmp_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HGU4428773.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32286..32978)
                     /gene="ygeA"
                     /locus_tag="ACHKYO_RS09585"
                     /old_locus_tag="pgaptmp_001917"
     CDS             complement(32286..32978)
                     /gene="ygeA"
                     /locus_tag="ACHKYO_RS09585"
                     /old_locus_tag="pgaptmp_001917"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HGU4428774.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33107..34525)
                     /gene="araE"
                     /locus_tag="ACHKYO_RS09590"
                     /old_locus_tag="pgaptmp_001918"
     CDS             complement(33107..34525)
                     /gene="araE"
                     /locus_tag="ACHKYO_RS09590"
                     /old_locus_tag="pgaptmp_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HGU4428775.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(34840..35601)
                     /gene="kduD"
                     /locus_tag="ACHKYO_RS09595"
                     /old_locus_tag="pgaptmp_001919"
     CDS             complement(34840..35601)
                     /gene="kduD"
                     /locus_tag="ACHKYO_RS09595"
                     /old_locus_tag="pgaptmp_001919"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HGU4428776.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(35631..36467)
                     /gene="kduI"
                     /locus_tag="ACHKYO_RS09600"
                     /old_locus_tag="pgaptmp_001920"
     CDS             complement(35631..36467)
                     /gene="kduI"
                     /locus_tag="ACHKYO_RS09600"
                     /old_locus_tag="pgaptmp_001920"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HGU4428777.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(36754..37935)
                     /gene="yqeF"
                     /locus_tag="ACHKYO_RS09605"
                     /old_locus_tag="pgaptmp_001921"
     CDS             complement(36754..37935)
                     /gene="yqeF"
                     /locus_tag="ACHKYO_RS09605"
                     /old_locus_tag="pgaptmp_001921"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGU4428778.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38190..39419
                     /gene="yqeG"
                     /locus_tag="ACHKYO_RS09610"
                     /old_locus_tag="pgaptmp_001922"
     CDS             38190..39419
                     /gene="yqeG"
                     /locus_tag="ACHKYO_RS09610"
                     /old_locus_tag="pgaptmp_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4428779.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            39879..40511
                     /gene="yqeH"
                     /locus_tag="ACHKYO_RS09615"
                     /old_locus_tag="pgaptmp_001923"
     CDS             39879..40511
                     /gene="yqeH"
                     /locus_tag="ACHKYO_RS09615"
                     /old_locus_tag="pgaptmp_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4428780.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            40845..41654
                     /gene="yqeI"
                     /locus_tag="ACHKYO_RS09620"
                     /old_locus_tag="pgaptmp_001924"
     CDS             40845..41654
                     /gene="yqeI"
                     /locus_tag="ACHKYO_RS09620"
                     /old_locus_tag="pgaptmp_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4428781.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            41647..>41755
                     /locus_tag="ACHKYO_RS09625"
                     /old_locus_tag="pgaptmp_001925"
     CDS             41647..>41755
                     /locus_tag="ACHKYO_RS09625"
                     /old_locus_tag="pgaptmp_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4428782.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNET"
BASE COUNT        10506 a        11147 c        10042 g        10060 t
ORIGIN      
        1 gttcaaatcc ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct
       61 gctctcccat aaaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtc
      121 gccccatcgg caaaatacgc tttaattccc gcaagaatag actccgcaac ttcctgctgg
      181 aaagtcgccg ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc
      241 agaatggagg gaatatcagg cgctttcagc acggcaaacc cggcctgttc aacctgattt
      301 ttatgcagct tgttgatttt accgagctta ttcagcaccg ctttaccaaa cttaaggctg
      361 tcggcaatgg tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg
      421 cttttgctca cgccaccaat caagtccgag gcgttctggg tctgtgccag atattttgcc
      481 gcagtactgg ttgcgccttt ggttgagagc gcaaacactg aggaaccgct cggctggcga
      541 ctggtaaagg cgtcggcatg gatagagaca aacaggtcgg cacgctgttt ctgggctttt
      601 gctacgcgca cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata
      661 ttgccctctt tctcgatcag agagcgcaga cggcgagcta tttgcaatac cacgtctttt
      721 tcgcgcgttt tgtatttccc caccgcaccg gagtcttcgc cgccgtggcc agggtcaagc
      781 ataataacaa taggacgatc gcgccctgct ttacccggtt gtggaccact ttgtgctggc
      841 ggcacctgct tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc
      901 tgcatatcct gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg
      961 accggcgcaa gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatccgt
     1021 acggtctgcg ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga
     1081 atttgtgccg ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc
     1141 acgcgttccg ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc
     1201 gtcacgcggg tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctaaccgca
     1261 gccaggctga cctgacttac gctcaatagc cacatagcgc ccgcgccttg cagtaaacga
     1321 cgacggctga ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc
     1381 tgtgtaacaa gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc
     1441 atctaataaa cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc
     1501 tcgcataaaa tttacacttg caccctgcga aaaaacagaa taaaaataca ataatttcga
     1561 ataataatgc aaaggggtgt gccgtggtaa aggaacgtaa aaccgagttg gtcgagggat
     1621 tccgccattc ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg
     1681 gcggtgaagc cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc
     1741 acagcctcgg catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc
     1801 tggctgcgca tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa
     1861 cactggaact ggtgaagcag gctgcgggaa cattgcaact ggatattact gctcgcctgt
     1921 cgatgagtct caataacacg ccgctacagg gcgcgcatat caacgtcgtt agtggcaatt
     1981 ttattattgc ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta
     2041 tccggcggat tgatgaagac gcgatccatc gtcaactgga cagcggtgca atagtgctaa
     2101 tggggccggt cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg
     2161 ccactcaact agccatcaaa ctgaaagctg aaaagatgat tggtttttgc tcttcccagg
     2221 gcgttactaa tgacgacggt gatattgtct cagaactttt ccctaacgaa gcgcaagcgc
     2281 gggtagaagc ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg
     2341 gcgcagtgaa agcctgccgc agcggcgtgc gtcgctgtca tttaatcagt tatcaggaag
     2401 atggcgcgct gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg
     2461 aaagcgccga gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt ctggagttga
     2521 ttcgcccgct ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg
     2581 aaatcgacaa attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct
     2641 atccgttccc ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc
     2701 gcagttcatc acggggcgag gttctgctgg aacgcattac cgctcaggcg aagcagagcg
     2761 gcttaagcaa attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat
     2821 ttaccccggt ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc
     2881 gtaaatccaa agtgttgatg gcggatttag ggtaagcgat gaaaattgtc ggatgcgaca
     2941 tgcgtaacac tcgtgcgtcg catccggcaa ttacgtttat tcccgcgaac taaacaacgc
     3001 cgccagacca ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tacgctcatc
     3061 ggcatacagc gacagacggc gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt
     3121 tactaccggc gtgcgttggc tcggtaaaat caacgccgca tggtcgaact ccgatccctg
     3181 cgatttatgt accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac
     3241 agacttaata ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag
     3301 cgcaataccg atatcgccat taaacaaccc aagcgcgctg tcattacggg caatcattac
     3361 cggtcgacct tcgtaccaac gagagtgcgg atgacgatga attttgcgct tctgttgcat
     3421 aaactgctca attcgctcat tcagtccagc cacgccaaac ggcccttccc gcagggcgca
     3481 caaaagttgg tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag
     3541 cagatccaga taacgtccat aacccgcaag agcttcctca agcatcgcaa tataatcttc
     3601 tccgctctgt aaaagccgtt tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac
     3661 tgccgtttta tcaccacggt tgatcgccgc agctaactga ccaatgccag aatcactgcc
     3721 gaaacgatag cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc
     3781 agttcctgcc ggaacgtgag ttcccgtcag gcggcttagc tgcccggcac gctcggcggt
     3841 aaagccggca ttggcataag cgcagatatc gcccagcaca gccccagcct caaccgaggc
     3901 cagttgatca cgatcgccga gaaagatcac tcgcgcatga tcgggcaagg cgtcgatcag
     3961 tctcgacatc ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg
     4021 cagcgggtta ccggcatgat gacgtaaacg ctggctaccc ggctgcgcac ccagcaatcg
     4081 gtgcaaagta ctggcatctt ccggaatgcg tttcttttgt tcatcggtca gcggtaactg
     4141 tcgcaaagcc ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc
     4201 cagacggata cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gtaacttcgc
     4261 cacggtggtc gttttaccgg tgcctgggcc gccggaaatc accgagatcc gccgcgtcag
     4321 cgccactgcc gctgcaactt tttgccagtt aatttcatcg cttactggaa aaagtttgtc
     4381 cagggtttgc gccagtagag cttcatcaac ctcaatggca tgattcacct cattgaagaa
     4441 gcgcgctacc gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag
     4501 gatcatcggc gtgggttcat ctcccctgct gaccgcttga gaagccagca agcattcttc
     4561 ccaattttgt agctcaccga tttcactgac acaggtcgcc aacagcggat gcgacgcctc
     4621 gttattttcc agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat catgacttaa
     4681 cagtgccgcc gcgagggtga cggcaggatg ttcatctccc gccacggtca gggcaaattg
     4741 cacatccagc gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt
     4801 cattacgcct cctccagggt cataccggca aacatctcat ccatcagggc aatcaacccg
     4861 gcgttgggtc gggttgtgta aatcccttgt tgcggatgtt ctttatcaac gccacgcagg
     4921 aacagataga taacgccgcc aaagtgacga tcatagtcgt aatcagcaat gcgatggcgc
     4981 agataacgat gcagcgccag ggtataaagc tgatattgca gatcatagcg gtgtgcctgc
     5041 atcgctgctg ccatagcctg ttgggtgtaa gccgaactgt cttcacccag ccagttggat
     5101 ttatagtcga gcaggtaata acgcccttcg tggcggaata ccaggtcgat aaagcctttt
     5161 aacatgccgc gtacctgcat gaactccagc ggcgggcagc cagcggatag cgggtcaaac
     5221 tggcgaatta gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg caggtaaaac
     5281 tccatctcca cctgtttatc gcggtcggaa agctgactca ggctaacacc cgtttcattg
     5341 agaggtgcct ggaggacagc cgtgatccac tcggtcaaca ccggttccca ctgcgattca
     5401 aagccgccga gttccagttt ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg
     5461 aggtcttcaa acaaactgtg caagaatgtc cccggtgacg caccgcgcgg gaactgatgc
     5521 ggtgttaacg tcggttcttc aacgacgctg accacgcctg cggcatcaac atccagccgt
     5581 ggcatcagat cctgagcgat accgtgacca cgctgctgca agccggagta gctggtgaca
     5641 cgccagttat cgccgggcaa tcgttgtagt gtcctcgcat tcagttctgc agttaaagca
     5701 tcattaacct gccagggctg gttatcacca gtttgtgccg tttgccaggc aatatcatca
     5761 tcgcataacg cttcaataca ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc
     5821 agcaaacgcc cgagcgcact ttggtggacg tcggtgtcac cttttttatc gccacgacgg
     5881 cgcaccagcg gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa
     5941 agcaaacgca gatcttccgc cagacgttcg acctccgcga ggtcgacgct ttctggcgca
     6001 gcgttaagat ccaaaactgc ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg
     6061 acgcggaaat tggtgataaa cggcagccag accaatggat attccagccc tttcgatttg
     6121 tggatcgtga caatctgcac cagatgttta tcactttcga gacgcatttg ttggctggag
     6181 gcattactgt ctggctcgag gatatgttgc gataaccagc gtaccagcgc atgttcactt
     6241 tccagctgcg ttccggcttc ttgtagcagt tcgctgatat gcaagatatc ggtaagacgc
     6301 cgctcaccgc ctgccgttgc cagcaagttt tcagcaatgt tacgcgccga catcagcgcc
     6361 cgcagcatcg gcataacgcc acgtttgcgc cagatttgcc gataaccatc gaactcttcg
     6421 actaccacat cccacgcatg ttcgtcattg ttcagcgttt cgatatccag cgcgttcagc
     6481 cccatcattg acgttgccag cgcactacgc agggtgttct cacgttcggg cgtcatcacc
     6541 gcctgcaaca accaaagcat ttcctgcgct tccagagttt caaaaacact gtcgcggttc
     6601 gaaaggtaaa cggaagggat ttccagcaac gttaaggcgt cgcgcacctg ggcggcctcc
     6661 tggcggctgc gcaccagcac actgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg
     6721 ttcatcagca acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca
     6781 catacctgcg ccatggtact ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc
     6841 cacattttca tcgcaggctg cgtttcacct ttaaacacaa aacgtaacgc ctgatttttc
     6901 ccggctgatt tcactggaat aaacggtatt tcgcgaaaca tgaacgcgtc atcagtctgg
     6961 ctgaaaagct tgttcacact gttcaccatt cctggtgcgg aacgccagtt ggtgtctaaa
     7021 gtgtagtggg cgtgaacttc gctacgcgcc ttcatataag tgaagatatc cgcaccccgg
     7081 aatgcatata tggcctgttt cgggtcgcca atcagcaaca atgcggtttc cggctgatgg
     7141 tgccagatac ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa ttcatcaatc
     7201 attgccaccg ggaatcgcgt acggatcgcc gctgccaaca cctcgccgct ttcgctacgc
     7261 agcgcggaat cgagccgact taacatgtca tcaaaaccca attcgccacg gcggcgtttt
     7321 tcacgcgcta ctgtttcgcg gatctcagcc aatgcgcggg ttatcaccag atcacggatc
     7381 gacaatggtt ctgcaagcag ttgttcgatc gcctcaaaca gtggatgtcg cggggtttcc
     7441 cccccggctt tcgtgcgatc ttctaagaaa cgctgagaga atttttccag cgactccggt
     7501 aactgataac tgttggtctc ttcttctgcc caggcgctga tcttctcgat ccatttagcc
     7561 tgattgctac ggttaaactt gcgtcgatca ataccagaag attcgatcag cgcatccagt
     7621 tcacccactg cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg
     7681 tggcgggaag ccagcgtttc atcatcgggc ggcggtgctt tgataaccgg tgcttcgcct
     7741 tgcagataac gattaatatc gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacc
     7801 accagggcaa tttcacgcgg cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc
     7861 tggtagcgta gcagagactc atcttcaatc agctgctgct caaacagcat accggattca
     7921 aaggcattca ggttgagcat gcgctggcaa aaaccgtgaa tagtaaagac tgccgcttca
     7981 tccatctgcc gttcggctaa caacaaccac tgcgcggctt gcgctttatc gtcgatctct
     8041 tccagcaggc gttcgtacag tggattgtcg gtggtttcac gcagacaggc gatgcgcaac
     8101 tcgtggatat tgctacggat acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg
     8161 accaccagca gttcttcaac ggtcagcggg cggggaaagg cggcggaacc gcctagtcca
     8221 agtaacaggc gcaaatagag cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct
     8281 tcaatcaggc gctcgccctg taagggcaag cgcaaaggat ctagtgtctc ggcgacatca
     8341 ctcattcttt tcactcatca ggggcattgt ttgctgcaac gcgctgacgt tctcccacac
     8401 tttccagcct tcaggatgca catattcggc tttcccgttc tggctgccgg aaatctgcga
     8461 caggatagcc atgccttgcg gctcgaccac cgcctgatgg aagaaatcag caagtttttg
     8521 cggcgtcagc agttttatct gggccacgat tttatcacgc gaatcgaagc gcatattgcc
     8581 gcgatcgaaa tctttactta acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag
     8641 catctgggta attaccgcct gctggatttg cgcaaactca tctggcttca tcgctcgcaa
     8701 ttttgcctct gcggttggga aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt
     8761 atcattgctt tgcaaaagga agcccatgcc ccactgacgc cccacgctca ttggaaacgc
     8821 aaacacggca tagcccaatt gttcttcggt acgcaactga ttgtagaacc acggctgtac
     8881 gatctgcccc aacagagagc tataggctga gctggtgtat tcatcgtagc cagtcggtac
     8941 aaataccgct gccagtgcgg agtcggtgct gttaccggct ttttcaaaga tgacggattg
     9001 ttttttatcg accactacat ctttgtttcg acaccactct gaaccatcag cgcccaactg
     9061 tttttgcaca tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat
     9121 aaactctggt cgagcccctg attttaaggc gtcgcgatag gccagcacct ctttcaacgt
     9181 aatggagggc aaaattttac gccgttcatc tcgcgagaag tacggcactt gcgagagcat
     9241 ctgcgcgggc ataatcgcct gctcaaacgc tttacccttt tctgcggaat ccatcatctg
     9301 gttataccag gacttcgcct gctcaagctg atcttccgta gcggtatagc taaagtaccc
     9361 ctcgagcaat gcctggaaca gctgcggcag acgctgggtg taaccattag cattaaccat
     9421 aaggccgttg ttagcgttgg tggaaaaact tatgccacca accgacgcct ggttgcttaa
     9481 ctgatcaagc gccagccctg cgagataatc attgagcgca aacatcacct gattgcgggc
     9541 gctgtccatg gctttcggat tacgcaaaat caggctgaca tcagctttgg gttcgctgga
     9601 aaaataacgg cttggcgcat acaccacgcg cagattcgac tcatcaacaa tcagctctgg
     9661 atggtcatat ttcttctctg acttaatcag cgagaaatcg tcaggaatat aggggttaag
     9721 ctctggcaaa gagagcgcaa tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc
     9781 gctgatttta tcgacctgat acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc
     9841 tttcgggctg atataccaga tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt
     9901 tactgcttta gcatcgtacc gatcggcaat attgactgca tccagcgtat gctcaacagg
     9961 aacgcgaatc atggtatctg ccagccattc gacgtaatcc atatcacggg tgattgacgg
    10021 ataacggaag tcgatatcca gcacattcgc cagttcatcg aagtattgtt tatcgatccc
    10081 tttttcacgt aacaaattga ggtagctaaa aatagccgcc acaacctgat cgcgattcgc
    10141 cagaccttta tcggttaaag acgcagagat cgctaatacg ccgctgttgc cgttgacgat
    10201 aggatcggag ttggcgctaa tgccctcaac taatccctgc ttttgcagcc agtcggaaag
    10261 tgtacctggg ctgcgattac caatcagata ggtaatcaat tcatcggttt tgctacggaa
    10321 tttcgccgag ttgttatcaa tacgaaactc tacgcgcaac actttacgcg gcagcgccgg
    10381 gacgtaatga atgataatgc ccttttgcgc gtcggtgact accggcacgg tgatttccgg
    10441 ttttttgctc tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc
    10501 cggcagcggt ttattgctgt aaataaccgc cttcatcaga ttggcggaat agtacttctc
    10561 gtggaaatct ttcagcgcct gctgcaccgg attaccaggt ttgtcgctta aagtttcgag
    10621 gttaccacca gaaaactttg aaccggggtg tgccgggtta atggtttctg cgctgacctg
    10681 tgccatgcgc atcccgtcac gcgtacgcgc catggttaat tcagcgttca ctgcattacg
    10741 ctcacgttcg gcgtatttct tgtcgagcaa gggttctgca atagcatcag ccaggcggtc
    10801 taccgcacca ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc gatacggcgc
    10861 agtgctggca ttgtgactac cgccgtgcat tttgagatat tcggccagac tgtcagcctg
    10921 cgggtacttt ttcgacccca tcagactcat atgttcaagg taatgtgcca gcccctggta
    10981 cgcctcggga tcttccagcg acccaacggg caccaccagc gccgagagcg atttaactgc
    11041 ctgcggatca gaaaccagca agaccaccat accgttatcc agacgtatag cctgatactg
    11101 gcggttatct ttatcacttt tacggatggt ttcctgaatc ggctgccatc ccgtttctgc
    11161 ctgacttaag ggtgcccaaa gggcaactaa caacaataat gctttgaacc aggtgctgcg
    11221 gggcattcac ggacctcata atcaacttaa ttttctgtcc agattcaaca cgttaacgca
    11281 aatcatctgc cagaatttaa tcttgtgctg cacgagtcag cctatgttta tataaccatc
    11341 agtccgtgac tggtgcgcat cataaagtaa gcggataaat tgcgcaattt ttatacagca
    11401 ctcatgactg attaaagcga aacagcggta acaggaaacg ttgcgactgt tcaacgatgg
    11461 cctccattgt ctctggtgtt aattgccgcc agagccgttg ataccagata tcatcacctt
    11521 cgccacgcac catcatgttg ccttcgtaag cctgcaggaa tttcgtacgg gctttttgca
    11581 acgtggaatc gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt tttagccacg
    11641 cgccgccact ttcaggtaac accagcaatg gcgcggacat cccttcacga tacccctcaa
    11701 tcagttgtga gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa cgccactcgc
    11761 cgtctttgcg tagaaaaagg cgactttcac cattaccacc gctggcacag tagacaaggt
    11821 gttccagcca aagttgcatt ccctgcgcca cacttaataa agagggacgc cagcgcaaca
    11881 agccatccgg ctgcacctgc ggcaaccagc cagttatctg cacaccgttg caggcgagat
    11941 caatttccat actctgcccc ggctggcgac aggcaatgac tctgtcggca agctgctgca
    12001 tctcctggca ctgtgtttcc cagaaaattt caccaaaagc gccatacggt aaatcccctg
    12061 ccgcccggaa gcggcggaac aagcgttcgg catcatcctg ctcaaccagt gcattcaata
    12121 actgctgatt gatttgataa cgactaagcc cttcaagaat aaatggctcg gtgtcgggga
    12181 tttcgctgtc ttcagtacgg aagttcacct gcaaacgcat ctggaagaac gcccgcaccg
    12241 gatgtgccca gaatcgttgt agcgtttcca gcggcacggt ttccggtaag gtaaacggca
    12301 gcggctgaac aaattcagaa tgtgctttac cagcctggct ggccgcaggt agccattcac
    12361 gagcatagct ttgtcgttcg cctggctggt agttttgtgg atcaaacggc atccgggtat
    12421 ggaggcaagt aagatgcgct tttacccttg cctcgctttc atcacagttg agcgcttcat
    12481 cgcccggtag ataatgactt tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg
    12541 ggaaacgttc actgttatcc tgaatggagc gcccgatata gctgatataa agtttttgtt
    12601 gcgcggaaat taacgcttcc aggaacagat agcggtcatc atcgcgacgg ctacggtcgc
    12661 cacgcttcgg tttctggctc atcaggtcaa agcccaatgg cgcaagctga cgtggataaa
    12721 cgccgtcgtt cattcccagc aggcaaacca ctttgaacgg aattgaacgc attggcatca
    12781 gagtacaaat gttaaccggt ccggcgagaa aacgctggct gatacgttct tgatccaggc
    12841 gctgtgccag ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta
    12901 aaccttcggc gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt
    12961 ccgcatccgg caggaagaag gcgttgagca tatcgcgaca aaccggcaac cactcttcca
    13021 gcggacgctc ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc agggaagcca
    13081 gatgccccac cagttctgca attaagccgc tcgattcatc ataaggtaga accgattgcc
    13141 actcgccctg cgcgctctcc atcgcgtaac ccagcaacat gcgcgtcagg ccaaaccgcc
    13201 aggtgtgttg accggtagcg gggagttcca gctcgcgaac gttgtcgtca tctatccccc
    13261 aacgaatgcc ggattcgttg acccactggc gtaaataacg cagcccttct tcggtgatgt
    13321 caaaccgcgc cgccagcacc ggcacatcca gcagcgccag cacatcctct gacacaaagc
    13381 gactgtcagg cagtgataac aggctgataa acgcttccag taccggatgt gactgccgcg
    13441 cacgacggtc ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta ccaaacactg
    13501 cctgaataaa cggactgtag ctgtcgatat cagccaccat cacgatgatg tcgcgcggag
    13561 taagtgtcgg gtcttcctcc agcatcgcca gcaggcgatc gtgtaaaact tcaacttcac
    13621 gctgcgggct atggcaaacg tggaaggtga tactgctatc cagtggatca agcgggcgtt
    13681 tgttatcgct acgggaaaac tcttcgatgt tcacaccagc aacggcgcgg ttttccagtt
    13741 ccagaatgtc agactgaatg ttatgcagca ggttatctgg catcacatcg acaaaggcgt
    13801 ccagctcctg actgctatcc agatcggaaa ggagatagat atagtcgcgc cccaacttgc
    13861 cccaggaggc cagtagcggg ttgccgacat cctgttcacc gtcgctgtta aagagctgtc
    13921 cggcattttc gctatcgcga aatagcggca attcgcgatc ttcaaaactg tgtcgacgct
    13981 gacgagtcag cagtttcgcc agataagcag gatctttaat atcgccccag taataacggc
    14041 aggggttggt aaacaggaga tggatttcaa tatgtttacc cagcgcctgg agcgcctgga
    14101 gataaacagg cggtaacgcg gaaataccgc atataaagac gcgcgaaggt aaccccggcg
    14161 ggcaggtcgt cgcggactcc agcgtttcga taaagcgctg atagagattg gcgcggtgcc
    14221 agcgcggttg cccgagttga tgggtatatt ccaccaacgc cttccacaac ggcgcttgcc
    14281 aggcctgtgc ttctcccagc ccttcaacca aatgtcctgt ttcccactgt gccagccagt
    14341 ccggacgata gaccagatac tggtcaaaca ggtccgccgc ttttgaagaa agctggaaca
    14401 gttttcgctt gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag tcttcgcgat
    14461 ccagcaattg cggcagcaga gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc
    14521 tctctttggg gatttccggt aacacccgga cgaacatatc ccagataaag ctcgcaggca
    14581 gcggaaaatc aatgtttgcc gcaataccaa acttttgcga cagggtcatt tgcagccact
    14641 gcgccatacc ggtactttgc accagaatca tctctggttc gaaaggatcg tccagccgtt
    14701 cgcgttcgac aataaactcc atcaacgctt ccagcacgtc cagacgattg gaatggtaga
    14761 cccttaacat agcggctcct gactactgac gattcgggca atgcaggcgg gtcatctcgc
    14821 cttctctgcc cccgggtgaa actagcgtaa cgctgatgct gacacatccc gcctgcgatg
    14881 tctgcattcg gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct
    14941 gccagccatg ccgccagagc tgttggtact ggtttctgct ggcaagactg ttcattaatg
    15001 ttcgctggaa acccgataac gcagtgacaa tcatcaccat caacaccatc gccaacatta
    15061 cctccggcag gctaaagcct tgttgattct tcagggaagc tgacataacg ccctctcttt
    15121 cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca atgtttccat caatgacttc
    15181 gcctgttcgc cacaacgaaa cgccttcata atcggcaatc aataaggctt cattatctgc
    15241 cagtaaacgc aaacaaacct gggcatcggt tccagcgtac tgcgagcact gaactgctgg
    15301 ctgcgtctgc caggagtgca tttttcccca cgccagcgcc gactgaacga tggcctggcg
    15361 gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct gttggctcat
    15421 tccttgtaat agcaagctac ctaaaaccag cagcatcagg accagagcca gtgacgaaac
    15481 acccttttcg cggttcacag gttgaatcct gtcacgctat agctggcatt caccacggtt
    15541 tgcggttcag acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg
    15601 acatcctgat gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta
    15661 tcccagccct taccttcaca ggatgtcgca ccgcgtagcg tttccagcac atgctccttc
    15721 agacgaaatc caatctggtc agactctttt accggttcgc gatcccagat accgttactg
    15781 ttcgcatccc actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg
    15841 gtacagctgc catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc
    15901 agccagattt catcttccag cgccagctta cgggtgctcg ttaaactttc acgctgtaac
    15961 gcaggcagaa agcgtgcagc ccccagcaac aatacgctac tgatcgccat agcaatcaac
    16021 acttccagca gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgac
    16081 aaagccggag tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga
    16141 agcgaatatg cccggcccat gcggtattgc gcaggccaaa gaaagcaagc gaaggtgtca
    16201 ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac
    16261 aggtattggc cccagcagcg gaactcacaa ggcaccataa cgtcccctcc ctgataacgc
    16321 tgatactgtg gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc
    16381 gcgcctggct ggcggtttgc catagccgtt gcgactgctg ccagtattgc cagccataga
    16441 gtccacttgc gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat
    16501 aaccacgttg tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag
    16561 tttctatttc ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt
    16621 tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa accgacgcac actggcgtcg
    16681 gctctggcag gatgtttcgt aattagatag ccaccggcgc tttaatgccc ggatgtggat
    16741 cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt
    16801 tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca
    16861 tatgattgct gtacaggtgc gtgtcgcccc ccgtccagac aaaatcgccc acttccagat
    16921 cacactgctg cgccatcata tgcaccagta acgcgtagct ggcaatgtta aacggcaggc
    16981 cgaggaaaac gtcacaggag cgctgataaa gctggcaaga gagtttgccg tctgccacat
    17041 agaactggaa gaacgcatgg cacggtgcca gcgccatttt atccagttcg cctacgttcc
    17101 acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt cagctggttc agtaccgtag
    17161 tgatctggtc aatatgacga ccatctggcg ttggccaggc gcgccactgt ttaccataca
    17221 ctggtccgag gtcgccgttt tcatcggccc attcgtccca gatggtgaca ttgttttcgt
    17281 gtagataagc aatgttagtg tcgccctgca gaaaccacag cagttcgtgg atgatggaac
    17341 gcaggtggca acgtttagtt gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct
    17401 gatgaccaaa aatggaaagc gttccggttc cggtacggtc gtttttctgt gtgccttcgt
    17461 cgagcacttt ttgcatcagt tctaaatact gtttcatggt tcctcaggaa acgtgttgct
    17521 gtgggctgcg acgatatgcc cagaccatca tgatcacacc cgcgacaatc atcgggatgg
    17581 aaagaatttg ccccatgctg atgtactgca cccaggcacc ggtaaactgc gcgtcgggct
    17641 ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac aagccagaga
    17701 cagctcccat tgggcgcggt ttacgaatat acaggttgag gataataaac agcaccacac
    17761 cttccagcag cagctcgtaa agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga
    17821 aaatggattg ccactgcggg ttggtttgca gcagcaaaat atcttctgta cgggagcctg
    17881 ggaacagcat ggcaaacggg aagtttgggt caacgcggcc ccacaattca ccgttaataa
    17941 agttgcccag acgcccggca ccaagaccaa acggaatgag tggtgcgata aaatcagaaa
    18001 cctggaagaa ggaacgttta gtacggcggg caaagataat catcaccacg ataacgccaa
    18061 tcaggccgcc gtggaaagac atgccgccgt cccagacacg gaacagatac agcggatcgg
    18121 ccataaactg cgggaaattg tagaacagaa cataaccaat acgtcccccg aggaagacgc
    18181 cgaggaagcc cgcatagagt aagttttcaa cttcattttt ggtccagccg ctgcccggac
    18241 gattcgcccg tcgtgttgcc agccacattg caaaaatgaa acccaccaga tacatcaggc
    18301 cgtaccagtg aagcgccacg ggtcctattg agaaaatgac cggatcaaac tccggaaaat
    18361 gcagatagct actggtcatc tgtcaccaca agttcttgtt atttcgctga aagagaacag
    18421 cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat agcacaaggg
    18481 gacctggctg gttgccggat accgttaaaa gatatgtata tgatccgcgc tataaccctc
    18541 cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc tgcaacctga tggcgaactt
    18601 cggtcgccag ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg gcataatcaa
    18661 tgcgccgcag caggtatttt acccgcgcta cagaacgtcc gttcatagac agatggcgat
    18721 acccaagccc aatgaggatt gccacgcaca tgggatcgcc cgccatttca ccgcacaaac
    18781 ggagatcgat tccatgtatt tccgcttccc gggcgatcat cgccagagct cgtaacattg
    18841 caggatgaag actgtcataa atgttcgcca cccgggtatt gttgcgatca acggccagaa
    18901 tgtattgagt cagatcgttg gtgccaacag agatgaaatc gacccgcttt gccagatgcg
    18961 gcagcataaa taccattgac ggcacttcca gcatgatgcc gatacgtggt ttgggaattt
    19021 cgtaaccgat catctcctcg acttcacgtc cggcacgttc aatcaggcgg cgtgcttcat
    19081 caacttcatc gaggcttgtg accatcggca acagaatatt caggttgccc gtagcggcat
    19141 tagcacgcag catcgcccgc acctggatca agaagatctc cggctgatcg agcgtaatgc
    19201 gaatcccacg ccaacccagg catggattct cttcgctgat cggcatgtaa ggcagctgct
    19261 tatctgctcc gacatccagc gtacgcaagg tgacgggttt atcattgaac atttgcagca
    19321 tcccctgata ctgcgccacc tgttcttctt ccgacgggaa accactttgc agcatgaatg
    19381 ggatttcagt gcgataaaga ccgatgccat caatacggct gcccagtttt tcttcatgtt
    19441 ccgggcttaa accagcattg agcatgactt ttatacgctc accgcttttt aactgcgcgg
    19501 gtaaattgac gtcatcttcc gccagacggc taagctcaat ctcttcacta attagccgct
    19561 gatattcttg cagcagtacc ggctccggat cgaccagcaa ttcaccgcga tagccatcaa
    19621 cgatcagcgt cctacgatgc agcaccgaag gctgaatatc cgcgcccatc acggtaggga
    19681 tccccagcgc acgtaccatg attgcagcat gggagttggc tgcgccatcg cgcacgacaa
    19741 cacccactaa gcgatcctgg ggcagctcag caagagttgt cgctgacagt tcatctgcca
    19801 ccagaatgaa acgttccggc caggcgttcg gcccttgatt agcgtcatca agatgaaaca
    19861 gcaatcgctg acccagcgca cgtaaatcgc cagcccgctc tttgagatag ttatcgctta
    19921 gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt taccgcccac tctgccaccg
    19981 agcctttatc aacctcggca aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg
    20041 agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt
    20101 agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg cgttccagag
    20161 ccggatccag cgttgatgcc tgatacacct gttccattaa aggtaacgtg gcatcctgcc
    20221 agccttcggc aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat
    20281 attgcccaaa caaggcagtc aactgcgact gagaaagaat agctgccatc tgggtggcaa
    20341 gcgtcaccag gaaggattct tcactttcgt catactggcg caactctcgt tgctgtacca
    20401 ccagtacacc aagcaactgg cgacgttgaa taattggtac gcctaaaaac gcgcggaaac
    20461 gttcttcttt tacggagggg atgtatttga agctggggtg cttttgcgca tctgcaaggt
    20521 ttatcggttc cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta
    20581 cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat caggtagtaa caacgtcgat
    20641 catgatcggc aaggtagacc gaacagacct cggtatccat cgcaagacag atgtcggtaa
    20701 ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc taccttttcg actatttcgc
    20761 gcaggcgagt gagcataatt ggcgtgactt aacctctttt acgtcgataa gcagatgcgt
    20821 tttgtggttt tggcgtattt tcctgcagtg acatcaccac gctcgcgaac tctttcatta
    20881 ccctacggta gacatcacgt ttaaatgaca ccacctgtct gaccggatac cagtaactta
    20941 cccatcgcca gccgtcaaac tctggtgtac tgctggtttg catattgatt tctgcatcgc
    21001 cgctcaccag ctgcaagaga aaccattttt gtttttggcc gatacaaacc ggcttcgtgt
    21061 cccaacgcac caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga
    21121 ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa ttcacggtac atcgcctgct
    21181 ctgcggattc tccggggttg attccgcctt gcggaaattg ccaggagtgc tgaccaaatc
    21241 gccgggccca cattacctgc ccctggcgat tacaaatcac gatacctacg tttgggcggt
    21301 agccatcgtc atcaatcacc ggactacctc aaaataaagc tttatatacg aatgattgtt
    21361 tcatactcca ggaagacggt aaaccactct ctgcagggca ttacacacta ataacaattg
    21421 aataactcac agttatgtgc agagttataa acagaggaag gggtggatag ccgtttttat
    21481 tcaccttttc tgtggataga gttgtgaaga actacggaat tactacggga aaacccggag
    21541 cattctgaat aagcccgata tagacatttt aaaatattca ttattagtca atgcattaca
    21601 tgtaatttga gtaatgaaaa agagtaattc gtgacccagg tcacacctct catttacggg
    21661 ttggcgaaag atcaaccaat gccgtattta tccacagaat gtgccactaa gttaagcact
    21721 gaaccactaa aaactggagt ttcatcgcac gtcaaggctg taaatggaaa cagtagtgga
    21781 ggtttttcac agttatccca gctttctgtg gataacatgg tgtaagatcc tgtttatttt
    21841 cagtgaccag atttggaaaa cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa
    21901 aaagcaatat aaatcaggga attgaacaac acatgtggaa aaagttacac tgcgaatatt
    21961 cggcacataa ttgctgtttg ttttttaatc aaggtatcat gacatgtccc aacctcgccc
    22021 actgctctct cctcccgaaa ctgaagaaca gttgttagcg caagcacagc aactttctgg
    22081 ttatacattg ggagaactgg cggcacttgc cgggcttgtt acgccagaga atttaaaacg
    22141 cgataagggc tggattggcg tgttactgga gatctggcta ggtgccagcg cagggagtaa
    22201 acctgagcaa gattttgccg ctctgggcgt ggaacttaaa actatccctg tggatagtct
    22261 tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg ttaacgggta atagcggggt
    22321 gacctgggaa accagccatg tgcgccacaa gctcaaacgc gtactgtgga taccggttga
    22381 aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc
    22441 gaatgaagag gaagaccgac agctacgcga agactgggaa gaattaatgg atatgattgt
    22501 tctcggtcag gttgagcgga tcaccgctcg tcacggggaa tatttacaga taagaccgaa
    22561 agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc cggggcgaac ggattctgac
    22621 gctgccacgc ggcttttatt tgaagaagaa tttcaccagt gcactactgg cccgtcattt
    22681 tctgatccag tagccatcgc tttgacctgc cgctttccgg gcatataatt accgcttcat
    22741 ttttttggca gggcttttta gatgttattt gcatggataa ccgatcctaa cgcctggctt
    22801 gcgctcggta cgctgacgct gctggagatc gttcttggga tcgacaatat tattttcctt
    22861 tctctggtgg tggcaaagct tcccacagca caacgtgctc atgcgcgccg tctggggttg
    22921 gcgggagcca tggttatgcg tctggcgctg ctggcatcaa tcgcctgggt tacgcgcctg
    22981 acaaatccgc tttttacaat attcagtcag gaaatttctg cccgtgattt gattctgctt
    23041 ctgggtggct tgttccttat ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt
    23101 gaagaagaag ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt gcagattatg
    23161 ctgctggata ttatctttag cctcgactcg gtgattacgg ctgtgggtct gtcagatcac
    23221 ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc
    23281 tcgattggtg attttgtcga acgccatcct tcggtaaaaa tgctggcgct ctctttcctg
    23341 attctggtgg gctttaccct gattctggaa agtttcgaca tccacgtacc gaaaggttac
    23401 atctacttcg cgatgttctt ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa
    23461 aagaatccgc tctgatactc cgtacgctct cctgcgggag ggcgtactca ctccttttac
    23521 cctcacagtt taagattttc ctgctttcaa gatatataac gtcggtttat aaacagacta
    23581 ttatcatagg taggccaaat gaggataacc ggatgaaaaa atgggcagta ataatttctg
    23641 cagtcggact ggcgtttgct gtttccgggt gttccagtga ttacgtcatg gcgaccaaag
    23701 atggccgtat gattttgacc gatggaaaac ctgaaattga tgatgatacc gggctggtga
    23761 gttatcacga tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta
    23821 ttgaacgtta acaaataagg tcagcatccg gctggcctta agatttttct ctttcccttt
    23881 tcccttccct ctgccatttt tatattcctc atgtcgtgat tataaaaagg aaacggctat
    23941 gcaatatcac cgtatacccc acagttcgct ggaagtcagc acgctggggc ttggcacgat
    24001 gacgtttggt gaacagaaca gcgaagccga cgcccacgca caactcgact atgccgtcgc
    24061 tcagggcatt aaccttatcg acgttgccga aatgtaccca gtacctccgc gccccgaaac
    24121 gcaagggtta accgaaacct acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa
    24181 gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc
    24241 ggatcaggcg ctggatcgga agaatatccg cgaagcgctg catgacagcc tcaagcgcct
    24301 acagactgat tacctcgatc tttatcaggt gcactggccg cagcgcccga ccaactgctt
    24361 cggcaaactc ggttatagct ggacggattc tgcgcctgcg gtttcgctgc tggatacgct
    24421 ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga
    24481 aactgcattt ggcgtaatgc gctacctgca tctggcggac aaacacgatc tgccgcgtat
    24541 tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt tttgaagtag gtctggcaga
    24601 agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt tcggcacgct
    24661 gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg
    24721 gttcacccgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc
    24781 cagacgtcat ggcctggacc ctgctcagat ggcgctcgcg tttgtacgcc gtcaaccgtt
    24841 tgttgccagc actctgctgg gcgcaaccac gatggatcag ctgaaaacta acatcgaaag
    24901 tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtgc atcaggttta
    24961 tacttatccg gcaccataag attaacgccg gttaaaaccg gcgttaaata ttaatgacgg
    25021 cgctgccaga tccacagcgc cgttattgcc agcgcaaaca gcgcaccgaa gccaatgcca
    25081 atgggcacga ccgggatgcc tatcattacc gccagcgagt aaatgcccag catcaacaac
    25141 atggcgctgt tttcgccaag gttttgtact gcaatcgcat tccccgcccc gacgcttttt
    25201 ttaccccgct cctgtagcaa cgcattgagc ggaacgacaa aaaagccccc catcacgcca
    25261 atcagcatca acaaggcata ggctggcagc agctcgtgtt gcagggaaaa aatcagtacc
    25321 accacgccaa tcaaaatccc ggctggcata cagcgtgaca cggtttccag cgtaactaac
    25381 ttcgctgccg cacctgcgcc aaccacgata ccaatcgcta ccatcgcgtt gagataggtg
    25441 ggcgtagcgt tatcggtaat gcccagcgcc accggtaccc acagcaccaa caggaaacgc
    25501 agcgtgacac ccgctcccca gaataaactg gtgcccacca gcgaaaaacg cgtttcacca
    25561 ttgcgccata gcgaggtgca ggcattcagg aaactgcggg tcatgttgat gagattccag
    25621 gactgccccg gacgcgccgc cgccagtttg ggaatgtaga tattggcaac gaccgcacca
    25681 ccgtaggcca gtgcgcatgc ggccagggcg acgaggacat gccagtcagc cagcacacca
    25741 ccggctacgg aaccgagcaa aatcgccgct atggtagaag cttccattaa accgttagct
    25801 ttcactaact tactacccgt ggttaattcg ccgagaatac cgtatttcgc cggtgaatag
    25861 gctgcagcac caacacccac cagcgtatag ccgagaaacg gattgatacc aaagcagata
    25921 ctggctgcgc ccagcagctt caggccgttg gcaaacatca tcacccggcc tttggcgaag
    25981 ctatccgcca cctgcccgac aaacggcgca aaaagaatgt aagcacctac aaacaccatt
    26041 tgcaggatgg gctggctcca ctccggatag aactgcgctt tcagtaacgc cagagtggca
    26101 aacagtaggg cattatcgcc aaacgcagag agaaactgcg ccacgataac cgctttcatc
    26161 cccttcgacc acaacgaagt gttagtgtgc actgactcac tcatcgtgtt gttccgcttc
    26221 gtctacccag cttttcaacg tgacaaagtc aggtttgccg ctgccaagta atggcatctg
    26281 tttcagatag cgaatatcgc gcggtacagc aagctccggc acgccgtgct cgcgggcata
    26341 ctgttgcaac ttatcgcgcg tcagttcgtt atctgtggtg aaaagcacca gtgcctcgcc
    26401 tttgctggca tcgctcttaa tcgcagtggc atggacttta tctggcgaaa caccaagtgc
    26461 cagttgttcc accatttcca gcgacaccat ttcgcctgca attttggcaa agcgttttgc
    26521 gcggccctga atctgcacaa agccctgctc gtcaaaacgc acaatatcgc cagtgtcata
    26581 ccagccgcgc tccatttcgc cgcgaacatt ctcggcggtg ggcacttcca gtacacctgg
    26641 cttctccacc cgcagatagc cgttcattat gttcggccct ttcagttgca ggcgtccgcc
    26701 ctcttcgata ccagggaccg acaacaggcg cgcatccatt cctggtagaa tacgccctac
    26761 cgtaccgggt ttcgccgcca tcggtacgtt gatagaaacg acaggcgcgc attcggtcac
    26821 gccgtagcct tcaaggatgc gcaggccaaa tttatcctgc caaagctgtt tggtactttc
    26881 ttgtaatttt tctgcgcctg ccaccacata gcgtagacga tagaagtcat acgggttggc
    26941 gaaacgcgcg tagtgaccga ggaaagtcga ggtgccgaac aacacggtgc aactgcggtc
    27001 atacaccaac tccggcacaa tgcggtaatg cagcgggctt ggataaagga acacttctgc
    27061 acctgtaagc agtggcgtaa acaggcctac cgtcagccca aaggagtgaa acagcggtaa
    27121 cgccgacata aagcgatcgt tggtggtgaa gtcggcaatc gttttaatct gctcgacatt
    27181 cgccagaatg cttttatggc tatggacgac gcctttcgga tggccttcag aaccggaggt
    27241 aaagaggatc agcgcctctt cttccggctg ctgtttaacc tgtgccagac gcggcatcag
    27301 caaatgagcg aagatccata ctttgtcggc agtggtgaca tctgctttta aatcttccag
    27361 atagacccag cgcacctgag taagttgctc cggcagatgc cagagtttgc ctttatcgag
    27421 aaactggcgg gaagtgaaga tggttttgat ttcagccgcc gtaatagcac tggtcagccc
    27481 ttttaccccg gcagtgtagt tcatcattgc gggcatgcgg cgacgggcga tggccccaaa
    27541 aatcactgcc gcactgatgc ctgcattggg cagcattaag ccgatgcgtt cgccttcaac
    27601 actgtatttt tcaaggatgc gtccaacaaa cagcgttttc gtaagcaatt tgcgatagga
    27661 gtctggggta aagttgacgt cttcgacaca tttcttcccg gctccgaagc ggtacattgc
    27721 actcagtaaa gattcgtaca gcgtttcacg cgggcgcacc gccattcgcg cttccatcat
    27781 tatttgatgc agcatttcgc cagcgatttt gcgacggtca cgggcacgcg gcgcatccgg
    27841 catcgccacc tgcgttggtg gcaaaatatg cagagtaatt tgcgggaaca agcgacgttt
    27901 aaccagacct ttcaggcggc tgaagtgcgt aagttccgcc ccttcaatac gcacaggaat
    27961 aaccgttgca ccagacttcg ccgcgacaaa acccgcgcca tcgtagattt tcatcagcga
    28021 gcctgtagtg gtgatgcgtc cttcagggaa aatcaccact ggtcggccct gttccaccag
    28081 acgtaccaga tgtttaatag ccataggttg cgtcgggtcg agaggaacaa agtcgataaa
    28141 tgatttcagc caacgcatat accactgttg gcttattgag gtgtaaacgg caaacactgg
    28201 acgcacaggt aaaaacagtc caagcaaaat gccatcaata aaagagacgt gattaggcgt
    28261 aattagaacg cgctcgccct tcagtgcctg ggggtcaccc gtaacgcgaa cgcgatacaa
    28321 aacacggcac aaatttcgaa aaaagctaaa aagcattcca actccctttg ctctgattca
    28381 gtaaaagcga atggagggag attacacgag ataaagaacg cgagcgacag taaattaggt
    28441 gcgaaaaaaa acctgcgcat ccgcgcaggt tggtgcaaga gacagggtac gaagagcgta
    28501 ccgaataatc tcaccaatca atacctctgg gatcttgatt gtggtctgca cgacgactct
    28561 tcgccagcga gaaaacgcaa aggaatgaag ggaaatgcaa cgaggtgtgt aaattgtcgg
    28621 ttactgttac agattgatga ccggcaaaaa aaaacctgcg catctgcgca ggctggtgta
    28681 attcatgtgc tcaacccgaa gttgacttca cctatcaata cctctgggat caccacttta
    28741 gcaacctgaa gccaaacgcc accagcgatc aatcgcaaca gcgtttcgca aagtgtaacc
    28801 aaaggtttgc aatctctttt tttgtcttac ttatttgcgt ttggctcaca ttcccacgat
    28861 gaaaacacgc caccccttga accaacgggc gttttccgta acactgaaag aatgtaagcg
    28921 tttacccact aaggtatttt catggcgacc ataaaggatg tagcccgact ggcaggcgtt
    28981 tcagtcgcca ccgtttcccg cgtcattaat aattcaccta aagccagtga agcttcccgg
    29041 ctggctgtgc atagtgcaat ggagtctctt agctatcacc cgaacgccaa cgcccgtgcg
    29101 ctggcgcagc agaccactga aacggtcggt ctgatcgttg gtgatgtttc cgatccgttt
    29161 ttcggtgcaa tggtgaaagc ggtcgaacag gtggcttatc acaccggtaa tttcttattg
    29221 attggcaacg gttaccacaa cgaacaaaaa gagcgtcagg ctattgagca actgatccgc
    29281 catcgctgtg ctgcgttggt cgtccatgcc aaaatgatcc cggatgccga tttagcctca
    29341 ttaatgaaac aaatgcccgg tatggtgctg atcaaccgta tcctgcctgg ctttgaaaac
    29401 cgttgtattg ctcttgacga tcgttacggt gcctggctgg caacgcgtca tttaattcag
    29461 caaggtcata cccgcattgg ttatctgtgc tctaaccact ctatttctga cgccgaagat
    29521 cgtctgcaag ggtattacga tgcccttgct gaaagtggta ttgcggccaa tgaccggctg
    29581 gtgacatttg gcgaaccaga cgaaagcggc ggcgaacagg caatgaccga gcttttggga
    29641 cgaggaagaa atttcactgc ggtagcctgt tataacgatt caatggcggc gggtgcgatg
    29701 ggcgttctca atgataatgg tattgatgta ccgggtgaga tttcgttaat tggctttgat
    29761 gatgtgctgg tgtcacgcta tgtgcgtccg cgcctgacca ccgtgcgtta cccaatcgtg
    29821 acgatggcga cccaggctgc cgaactggct ttggcgctgg cggataatcg ccctctcccg
    29881 gaaatcacta atgtctttag tccgacgctg gtacgtcgtc attcagtgtc aactccgtcg
    29941 ctggaggcaa gtcatcatgc aaccagcgac taaccgcagt taaagcaatt ccagcgccag
    30001 taattcttcg atggtctggc gacggcgaat caaccgcgcc tgaccattat caaacagaac
    30061 ttctggtaac agcggacggc tattgtagtt ggatgacatt gatgcgccat atgcccctgt
    30121 atcatgcagt accagataat cacccgcctt cacttccggc aaggcgcggg tttcaacatt
    30181 tcccccttcc tgctgggtaa agacatcgcc cgattcacat aatggcccgg cgacgacggt
    30241 ttccaccgtt ggcgcgtgtt ccagagaacg accatcagct gccagggcac tgatatggtg
    30301 gtaactaccg tacattgccg ggcgcatcag atcgttgaac ccgacatcaa ccagcacaaa
    30361 gtggcggcta cccatttgtt tgacgctccg cacctgagta attaacacgc cagactgcgc
    30421 taccagaaag cgacccggtt caatttccag tttcacaggg tggcccaaat ggcgagcgat
    30481 ttgctcacgc gcggcattcc acagaccata ataatgttcg gtatcaaccg cctcttcacc
    30541 ctgttgataa ggaatagaaa gcccaccgcc cgcagaaata gcctgtaaat cctgaccgaa
    30601 ttcgatgacc tgacgtacca tagcaccaca cacctgttcc agatgggcat aatcaacgcc
    30661 agaaccaatg tgcatgtgaa tgccgaccag ctgcaaatga tgacgttgta tcacgtccag
    30721 tgcggcgggc agatcggtgt accagatacc gtgcttgctg ttttcgccac cggtattggt
    30781 tttttggcta tgtccgtgac caaaccccgg gttaacgcgc agccataccc gatgccctgg
    30841 cgaaacctgg cccagttggt cgagcatatc aacagaaccc gcattcaccg gaatttgcaa
    30901 ttcactgacg cgttcaagcg tcgcctgatc gataacatct gccgtaaaaa caatatcatc
    30961 ggggtgcgtt tgcggattgt aacccgccgc caacgcacgc tctatttcgc ctaacgagac
    31021 ggaatccact ttcacgccct gctcacgcat taagcgcaaa atatgaatat tggaacaggc
    31081 tttctgtgca aagcgcacca catcaaactg tttcagcgct gcaatctgcc gacgaataat
    31141 ttgcgcatcg tagacccaca ccgggcagcc aaattcagcg ggcaaacgca gcagattttc
    31201 ggcggtgaga tcggtatcgg tgctgaacag tgaatgtggc ataacaaact ccagataagt
    31261 gcttttttat gattacgcca catcataaaa agaataaaaa atatcgattt atgtcgagtc
    31321 tatgcaaaaa tgatatggat taccggattg cgagagagcg ctaatggccg ccgttaactt
    31381 acgtcatatt gaaatttttc atgcggtaat gaccgccgga agcctgactg aggcagcaca
    31441 cctgctacac acctcacagc caaccgtcag ccgcgaactg gcgcgctttg agaaggtgat
    31501 cgggctaaaa ttgtttgagc gcgtacgtgg acgattacat cctaccgtgc aaggactgcg
    31561 tctgtttgaa gaagtgcaac gatcctggta cggactggat cgcattgtca gcgccgcaga
    31621 aagtctgcgc gagtttcgcc agggagaact gtctattgcc tgcctgccgg tcttttcgca
    31681 atctttttta ccgcagctcc tgcaaccctt tctggcacgt tatcccgatg tcagcttaaa
    31741 tatcgtgccc caggaatcac cgctacttga agagtggctc tcggcccagc gtcatgattt
    31801 aggactcact gagacactcc atacgcctgc gggaacagaa cgtaccgaat tactctcttt
    31861 agatgaagtg tgtgtgttac ctccgggtca tccgctggcg gtaaaaaagg tattaacgcc
    31921 ggatgatttt cagggtgaga actacatcag cctttcccgt actgacagct atcgccagtt
    31981 gctggatcag ctatttactg aacatcaggt taaacgacgc atgatcgtag aaacccacag
    32041 cgccgcgtca gtctgcgcaa tggtacgggc gggggtaggt gtttcggtgg ttaacccgct
    32101 caccgcactg gattatgcgg caagcggttt agtggtgcgg cggttcagta ttgcggttcc
    32161 gttcaccgtc agcctgatcc gccccctgca ccgcccgtca tcagcgctgg tgcaggcgtt
    32221 tagtgagcat ttacaagcgg ggttaccgaa actggtcact tctcttgacg ctattttgtc
    32281 gtcagctacg acagcataaa agcgacagca tcctcggcat ggatcgccgc agtatcaaac
    32341 acaggcaaaa cactgcgctc ttctggcacc agtaaaccaa tttctgtgca gccaaaaatg
    32401 acgccctgtg cgccctgttc tgcaaggcga gcaatcactt gcgcataata agcgcgtgac
    32461 gcttcggtaa attgccccag acacagttct tcaaaaataa tctgattaat tttcgcccgt
    32521 tcatccgctt caggaataag acaattgatg gaaaattgtt ccgtcagccg cccgcgataa
    32581 aaatcctgtt ccatggtgta acgcgtaccc agcagcgcca cacgagtcat tccggccccg
    32641 gtaattgcac gtccggtggc atccgcaatg tgtaagaaag gcagagtgca acgtgactca
    32701 atggcatccg ccactttatg catcgtattg gtacataaca caataccttc tgcgcccgcc
    32761 cgctgtaagc caagcgccgc ctcagccaga atgtccccgg ttttatccca ttccccgcga
    32821 cgctggcact cttctatttc atgaaaatcg acgctatgta gcagcacttg cgcagagtga
    32881 agcccaccaa gccgctgttt aatgccttca tttatcagac gatagtaagg aatggtggat
    32941 tcccagctca ttcctcccag caaaccaatt gttttcattc ctctctcctg atgtgtgtta
    33001 ccgcaacaag gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccgatctct
    33061 attaacgaaa aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat
    33121 ttctcaactt ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg
    33181 tttccggaat gagccagaaa gtgatgccca caaacgcaat gttcagcgca gtgtagagcc
    33241 agaacgtacc ggcagcgcca atgctatcaa gcagtgtcag gaaggtcgcg ccgataatca
    33301 tattcgacac ccagtttgtc gtcgtcgaac aggtaatacc gaaatcgcgg catttcagcg
    33361 gctgaatttc agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa
    33421 tacacatcat cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat
    33481 caaactgcat caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa
    33541 ttttcagagc cggtttacga cctgctttat ctaccgtaaa gaccgcaatg aaggtcgcga
    33601 acataaaggt cagtcccacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc
    33661 ccgccatttt gaagatacgc ggcgcatagt acatgatgat gttcataccg gtaaactgct
    33721 gcatcgcctg caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa
    33781 acagtgccca accgccttgt tttaacttca ggctttcacg aatttcgttg agttcttctc
    33841 gcgctttttc cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc
    33901 ccttttccgc cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagga
    33961 ctgctggtaa agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg
    34021 tatcggataa aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca
    34081 tcttaccgcg aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg
    34141 cgatcccgac agcaatgccc agcaccacac gagcggcgat taacatctct acgctggtcg
    34201 caaaagcgga ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt
    34261 tacgacccag gcggaacgac agccaaccat taaacagcgc accaattgct gcaccgagca
    34321 tcatgctact gaccacccat tcctgcaaac ggctggtcag cacaaagtga tcggtaatga
    34381 acggcaacgc tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag
    34441 cagctaccga aacaaacata ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag
    34501 cagattccgt attgatagta accatttttt cctgccagca gagagtaaga catagtgaaa
    34561 aaatacgtga acaattcaca caggtgtcag gtcggaaaca gcataaatat ggattaaatt
    34621 gctgcggcat gtcgttatgt gatggatatt ccaattttca aattaagttg aattattgag
    34681 attattatta accacctaat tttacagcag ataaaattca taaagttcat taattgataa
    34741 ttgatatgga ttatttcata accatgatat ggattatgat gatctacagg tataaaaaac
    34801 cctgccatgc ggcagggtca taaaagtaag aagaatgaat taacgcgcca gccaaccgcc
    34861 atccacggca atggtataac cattcacata atctgaagcg ctggaggcaa ggaacactat
    34921 cggccccatc aggtcactcg gcagtcccca acgaccagct ggaatgcggt cgagaatttc
    34981 cgcgctacgt tgttcatctg cccgtagttg ttgagtattg ttggtcgcca tgtaacccgg
    35041 agctatcgca ttaacattaa tgttgtgttt agcccattcg ttcgccatca atcgcgtcac
    35101 acccatcacg ccgctttttg atgcggtata agaaggcaca cggatcccgc cctggaagga
    35161 gagcattgac gcgatattga taatcttgcc gccattgcct tgcgcgataa agtgtttcgc
    35221 cgctgcctga gacatgaaga atacgctctt gatattcagg ttcatgacat cgtcccagtc
    35281 cttttcgctg aactcgagag catcttcgcg gcgaatcaat ccggcgttat tcaccaggat
    35341 atcaatatga ccaaactccg ctaccgcgcg atccagcagt gctggaatac catcaatctt
    35401 tcgcagatcg gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct gcttgatggt
    35461 ttcagtaggt tcaacgatgt taatgccaac aatgtcacag cccgcttgcg ccagccccaa
    35521 cgccatcccc tggcccagcc cagtatcaca gccagtgacg accgcaactt taccttcgag
    35581 agaaaatgca cttaaaatca taacaatacc ttattcgttt atgcccacaa ctagcgcaaa
    35641 tctttaacgg ccacatggtc catatcatca aagacctggt tttcaccgac catgccccag
    35701 ataaaggtat aagctttggt tccgacgccg gaatgaatcg accagctcgg ggagatcacc
    35761 gcctgctcgt tatgcatcac aatatgacgc gtttcttgcg gctgccccat catgtggaaa
    35821 acgcaggcgt catcatccat attgaaatag aaataaactt ccatccggcg ctcgtgagtg
    35881 tgacacggca tggtgttcca caagttacct ggagccagct ccgtcagccc catactcaat
    35941 tggcaggttt ccagcacatc cgggacaaaa tatttgttaa ttgtgcgacg gttactggtg
    36001 aggttatcgc ctaacgtgac tggagatact tcgtccggtg tgactttttt ggtgggatac
    36061 gtcgtatgcg cgggtgcgca attgtaataa aactttgccg gagtggcggt atcaatactg
    36121 gcaaagacaa cttcttttgc acctttaccc acatacaggg cgtcgcggtg accgatttca
    36181 tagcattggc catcgacagt aatcgtaccg gcaccgccaa tattgataac acctaactcg
    36241 cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa cttccccgcc aacggaaacc
    36301 gttttagtta tcggcataat gccgccaaca ataattcggt caatgtggct gtaaaccatg
    36361 gtgtactcat cggcgacaaa tacctcttca accaaaaatt cattgcgcag cccctgggta
    36421 tccagcgttt ttgcgtgcgc actgtggatg ctctgtctta cgtccacatc aacctccgat
    36481 aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt tctctataaa
    36541 gggatgatat gcccatgggc tagggttttc aattaaaaat aaaacaatgt ttcattttta
    36601 agttaaggat taaaaaaagt gcagtgtcga tcacgaatat gtcatgagaa tggaaaaatg
    36661 agggagccca gaaaatgtat gtcgacttgc cagccatcat tattcaacag attaatgacg
    36721 gcagatattt atcggtaagg aggaaatgca gagctactcg tcacgttcaa tggtcaatgc
    36781 cacgccctgg cccccgccga tacaaagcgt tgccagccct ttgcgggcat tacgtttcac
    36841 catttcatga accagagaaa ccaggattcg gcaaccggaa gcgcctatcg ggtgaccgag
    36901 tgcgatcgcg ccaccattga cattgacccg acgctcatcc cactcaagca tcttgccaac
    36961 cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata agatcgacat cagccaactg
    37021 ccagcctaca cgctccaggc aacggcgggt cgcatacacc ggcgcaattc ccatcaatgc
    37081 cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc agcacgggta aattcaacgc
    37141 tcgtgctttg gcttcgctca tcatcattac cgcagctgcg ccatcgttta tggatgatgc
    37201 attccccgcg gtcacagaac cgagactatc aaatgaagga tttaaacggg ctaaaccttc
    37261 tgcactggcg tcagtgcgtg gctgttcatc ggtatcaaca accaacgtct gaccgttact
    37321 ttggatcatt accgggacga tctcatcttt aaatcgtccg gcgtcaatcg ccgctcgcgc
    37381 tttttgttgc gagctaagtg cgtaagcatc ctgcaactga cggctgatgc catattcgcg
    37441 agccagattt tcggcggtga caccaatatg ataatcattg aaggcatccc acaacccatc
    37501 atgcaccaga ctgtcaacca actggctatt gccaagctgt gcaccggtgc ggctatcagt
    37561 cagaacatgt ggtgcgcggc tcatgttttc ctggccaccg gcgatgacaa tatcagcctc
    37621 gccacactgt atcgcctgag tagccagatg cagtgcttta agcccagaac cgcaaacgtc
    37681 attaatagtg attgcagaaa cgctattagg aagaccacct ttaatagccg attgccttgc
    37741 cggattctgc cctgcccctg cagtcaacac ctgaccaaga attacttcat ccaccgcata
    37801 tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc agactaccaa gttccacggc
    37861 ggaatgaccc gctaacgcac cgcgaaagca gccgataggt gtccgtaacg ccccgacaat
    37921 cacaacgtct ttcatcacaa cctcgcacca attaacagtg caatagtaaa tgattgttaa
    37981 ttacagttat cttaattgtt taaaaaaagt gatttttatc acaaaggaaa tatgcctgag
    38041 cagtagtcag agtcataact ggcacgtaag gtttgcaacc actaacccac caatagaggg
    38101 gtagataggg cgttaatctc ccatacttaa cctggtttat ggtaaattgc cctccatttt
    38161 gtttaatttg tagatgatac gttcagataa tgtctaatat ttggtctaaa gaagaaactc
    38221 tgtggagttt cgcgctctac ggcacagccg ttggtgcagg cacgctcttc cttcctattc
    38281 agttaggttc ggcaggggct gtggtcctgt ttattactgc tctggtcgcc tggcctttaa
    38341 catattggcc acataaagcc ttatgccagt tcatcctctc atcgaaaaca tcagcaggtg
    38401 aagggataac gggcgcagta acacactact atggcaagaa gattggtaat ctgattacca
    38461 cgctgtactt catcgccttt tttgtcgtcg tgttgatata tgcagtggca attaccaact
    38521 cacttacgga acagctggca aagcatatgg ttattgatct tcgcatccgt atgttggtga
    38581 gtctgggtgt tgtattaatc ctgaatctca tttttctgat gggacgccat gcaaccattc
    38641 gggtaatggg ttttttggta ttcccattga ttgcctattt cttatttctt tccatttacc
    38701 ttgtcggtag ttggcaacct gatctattaa cgacccaggt agagttcaat cagaataccc
    38761 ttcaccagat atggatatcg attcccgtga tggttttcgc ctttagccat acgcccatta
    38821 tttctacgtt tgccatagac agacgtgaaa aatatggcga acacgctatg gataaatgca
    38881 aaaaaattat gaaagtcgct tatctcatca tctgcataag tgtactgttc tttgtcttta
    38941 gctgcctgct ttctattcca ccttcgtata ttgaagcggc taaagaagaa ggggtcacca
    39001 ttttatcggc gctttctatg ctgccgaacg ccccagcatg gctgtcaatt tccgggatta
    39061 ttgtcgcagt agttgcgatg tcgaaatcat tcctgggtac gtactttggc gttattgaag
    39121 gtgccacgga ggtcgtcaaa acaacactac agcaggttgg tgtaaagaaa agtcgtgcat
    39181 ttaaccgcgc actatcaatt atgttggtat cgctgatcac cttcattgtt tgttgcatta
    39241 acccgaacgc gatttcgatg atttacgcga tcagcggccc gctcattgcc atgatacttt
    39301 tcatcatgcc tacgttgtca acgtatctga tcccggcgct taaaccctgg cgttccatcg
    39361 gaaatctgat tacgctgatc gtgggtatcc tgtgcgtatc ggtaatgttc tttagctgat
    39421 atccattgtg aatggttcaa aattgctaca attttgaacc attccagttt tcataatgtt
    39481 cattcgttat tattccgcac tatcacttcc ctcatcatat cctagtatta tcagttagtt
    39541 aaacacacaa agagatctga acatcttaaa taagactatt taagatgcat aacttagatt
    39601 cgcaagatat cttccggtca ttcaaaaaca atttgcgata tagatattct cataccatga
    39661 gatagttatc tgaaccaccc tcaaccaggt aagattaaat cagtgaaaca taatcatatt
    39721 taataacgag aaatgcattt taaaatgtct taatcagaat tatagcaaat acagattaat
    39781 cgcaattttt cccagcaaaa ttacaaaatc atggtgtatt gacatcatca atcaccaatg
    39841 aaattatgca attatatacg gatagggagg ttcttaacat ggggctttgt agtcgttata
    39901 aaagtcttac atgcaatagt tgctcaatgc attgccaaat aatgccagaa gagtcaccgc
    39961 gtttgcagta ctgcgcaaac tcgtgttttt gtatgtggcc cgaagaaagt tcatatttta
    40021 atcgtggtgt ggtagaaggt atcttaacaa aaaaccacaa tgcgagatta agcggatata
    40081 tttttgtcga tttttcagta agttttttac gtctattcct tgaaaaagac tggattgact
    40141 atcttgcaag tactgacatg gggattgttt tagtcagcga ccgcaatatg caatcgttag
    40201 ctaattactg gcgaaaacac aactcagcaa tatctgctgt catatataat gatgatggtc
    40261 ttgatgttgc caatgaaaag atcagacaac tatttattgg tcgttattta tcatttacca
    40321 gaggtaacac attaactcag atggaattca ccataatggg gtatatggtt tctggttata
    40381 atccatatca gattgctgaa gttctggata tggatatccg tagcatctac gcgtacaagc
    40441 aacgaatcga aaagagaatg ggtggtaaaa taaacgaatt atttattcgt tcacattcgg
    40501 tccaacattg atactcaacg accagccaga atcatactct gtttatacgg gcatttattg
    40561 aaatgtccgt attttataaa taggccgtcc cctcaagagt aaacaccata gtatttaaaa
    40621 ccatcacacg aaaaattcag aagcattacg aaatatgaat ttacaaacaa tagtggcata
    40681 aatgttaacc atgttaattt acgtaaagtt ttacgttgca acattaaagc ctcatttcaa
    40741 tcatcatgat aaatataaaa ttaatatata tttatgccgt aaataccaat atacttagca
    40801 aactatgtga tctccatttc gattgattta gtgtttattg acgtatgtac tggattatta
    40861 acgataatat cgagttctgg cctgagcacc gaaaattaat atcggtacat aacgccgatc
    40921 ttaacgtcgt tctgacaacg ccagccagtc gatgtttatc acttctactt gaagcttttc
    40981 ctgatgtggt tgcacaacaa gattttttca ccagagtctg ggaagaagaa ggtatgcgtg
    41041 tgcctactaa cacgttatat cagaacatat ccattatcag acgcggattt cgcgctgttg
    41101 gtgatactac ccactcgcta attgcaaccg tgccgagaag aggattcaag atccataatg
    41161 acattaacat acaaaatcat gtaataaact cgtcaacaga cgcacataca cacaatgccc
    41221 cacctgccat aaaagttaat gcgggataca aagagagcat tggtggcgca aagaatttca
    41281 ataacaaaat cctcaaacat ataaaaagtc atctaattat gttgagcgca tttgtcatag
    41341 gcgcatattc tgcatattgg ctatggaata ataatcaacc gaagccattt ttcaaggatt
    41401 acaagactgt tgcggaaatt aatggctgcc attttaatgt aacagaagat acaattgacg
    41461 ggttgaaaga gttcgataaa tataaaacac ggatactgga ttcggggatt aactgtaaaa
    41521 aacatccgtg gttatacttc cctcttgcaa aatcctcacc ggggatgatt gttatggcat
    41581 gcaataaaaa ttataaccaa catgaagtgg cagactgttt aaccctgtct taccgtgagg
    41641 ttaatcgtga ttgattataa aaaaaactta ttatttattt tagtttttat cagtgggttt
    41701 attctcttca ctgtctattc ttacactgca gaaaaaatga tatataacga aacat
//
LOCUS       DAZGAN020000032        41452 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGAN020000032 DAZGAN020000000
VERSION     DAZGAN020000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 41452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.032"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..340
                     /locus_tag="ACHKYO_RS09630"
                     /old_locus_tag="pgaptmp_001926"
     CDS             <1..340
                     /locus_tag="ACHKYO_RS09630"
                     /old_locus_tag="pgaptmp_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU4428783.1"
                     /translation="FLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHN
                     IFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWL
                     LVDPSSTAHE"
     gene            complement(324..962)
                     /gene="ytfB"
                     /locus_tag="ACHKYO_RS09635"
                     /old_locus_tag="pgaptmp_001927"
     CDS             complement(324..962)
                     /gene="ytfB"
                     /locus_tag="ACHKYO_RS09635"
                     /old_locus_tag="pgaptmp_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HGU4428784.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1181..1801
                     /gene="fklB"
                     /locus_tag="ACHKYO_RS09640"
                     /old_locus_tag="pgaptmp_001928"
     CDS             1181..1801
                     /gene="fklB"
                     /locus_tag="ACHKYO_RS09640"
                     /old_locus_tag="pgaptmp_001928"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4428785.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2110..3522
                     /gene="cycA"
                     /locus_tag="ACHKYO_RS09645"
                     /old_locus_tag="pgaptmp_001929"
                     /gene_synonym="dagA"
     CDS             2110..3522
                     /gene="cycA"
                     /locus_tag="ACHKYO_RS09645"
                     /old_locus_tag="pgaptmp_001929"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HGU4428786.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3567..4229)
                     /gene="ytfE"
                     /locus_tag="ACHKYO_RS09650"
                     /old_locus_tag="pgaptmp_001930"
     CDS             complement(3567..4229)
                     /gene="ytfE"
                     /locus_tag="ACHKYO_RS09650"
                     /old_locus_tag="pgaptmp_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HGU4428787.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4337..5302)
                     /gene="ytfF"
                     /locus_tag="ACHKYO_RS09655"
                     /old_locus_tag="pgaptmp_001931"
     CDS             complement(4337..5302)
                     /gene="ytfF"
                     /locus_tag="ACHKYO_RS09655"
                     /old_locus_tag="pgaptmp_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4428788.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKYPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5410..6270)
                     /gene="qorB"
                     /locus_tag="ACHKYO_RS09660"
                     /old_locus_tag="pgaptmp_001932"
     CDS             complement(5410..6270)
                     /gene="qorB"
                     /locus_tag="ACHKYO_RS09660"
                     /old_locus_tag="pgaptmp_001932"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU4428789.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6359..6739
                     /gene="ytfH"
                     /locus_tag="ACHKYO_RS09665"
                     /old_locus_tag="pgaptmp_001933"
     CDS             6359..6739
                     /gene="ytfH"
                     /locus_tag="ACHKYO_RS09665"
                     /old_locus_tag="pgaptmp_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGU4428790.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6868..8811)
                     /gene="cpdB"
                     /locus_tag="ACHKYO_RS09670"
                     /old_locus_tag="pgaptmp_001934"
     CDS             complement(6868..8811)
                     /gene="cpdB"
                     /locus_tag="ACHKYO_RS09670"
                     /old_locus_tag="pgaptmp_001934"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HGU4428791.1"
                     /translation="MIKFSATLLATLITASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLTAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            9001..9741
                     /gene="cysQ"
                     /locus_tag="ACHKYO_RS09675"
                     /old_locus_tag="pgaptmp_001935"
     CDS             9001..9741
                     /gene="cysQ"
                     /locus_tag="ACHKYO_RS09675"
                     /old_locus_tag="pgaptmp_001935"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HGU4428792.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(9731..10288)
                     /gene="ytfJ"
                     /locus_tag="ACHKYO_RS09680"
                     /old_locus_tag="pgaptmp_001936"
     CDS             complement(9731..10288)
                     /gene="ytfJ"
                     /locus_tag="ACHKYO_RS09680"
                     /old_locus_tag="pgaptmp_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU4428793.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLNKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            10613..10819
                     /gene="ytfK"
                     /locus_tag="ACHKYO_RS09685"
                     /old_locus_tag="pgaptmp_001937"
     CDS             10613..10819
                     /gene="ytfK"
                     /locus_tag="ACHKYO_RS09685"
                     /old_locus_tag="pgaptmp_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HGU4428794.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(10881..12224)
                     /gene="paeA"
                     /locus_tag="ACHKYO_RS09690"
                     /old_locus_tag="pgaptmp_001938"
     CDS             complement(10881..12224)
                     /gene="paeA"
                     /locus_tag="ACHKYO_RS09690"
                     /old_locus_tag="pgaptmp_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGU4428795.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(12547..13185)
                     /gene="msrA"
                     /locus_tag="ACHKYO_RS09695"
                     /old_locus_tag="pgaptmp_001939"
     CDS             complement(12547..13185)
                     /gene="msrA"
                     /locus_tag="ACHKYO_RS09695"
                     /old_locus_tag="pgaptmp_001939"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HGU4428796.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDQGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            13391..15124
                     /gene="tamA"
                     /locus_tag="ACHKYO_RS09700"
                     /old_locus_tag="pgaptmp_001940"
     CDS             13391..15124
                     /gene="tamA"
                     /locus_tag="ACHKYO_RS09700"
                     /old_locus_tag="pgaptmp_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HGU4428797.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            15121..18900
                     /gene="tamB"
                     /locus_tag="ACHKYO_RS09705"
                     /old_locus_tag="pgaptmp_001941"
     CDS             15121..18900
                     /gene="tamB"
                     /locus_tag="ACHKYO_RS09705"
                     /old_locus_tag="pgaptmp_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HGU4428798.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDDKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVNTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLENDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            18903..19244
                     /gene="ytfP"
                     /locus_tag="ACHKYO_RS09710"
                     /old_locus_tag="pgaptmp_001942"
     CDS             18903..19244
                     /gene="ytfP"
                     /locus_tag="ACHKYO_RS09710"
                     /old_locus_tag="pgaptmp_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HGU4428799.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            19456..19707
                     /gene="chpS"
                     /locus_tag="ACHKYO_RS09715"
                     /old_locus_tag="pgaptmp_001943"
     CDS             19456..19707
                     /gene="chpS"
                     /locus_tag="ACHKYO_RS09715"
                     /old_locus_tag="pgaptmp_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HGU4428800.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            19701..20051
                     /gene="chpB"
                     /locus_tag="ACHKYO_RS09720"
                     /old_locus_tag="pgaptmp_001944"
     CDS             19701..20051
                     /gene="chpB"
                     /locus_tag="ACHKYO_RS09720"
                     /old_locus_tag="pgaptmp_001944"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HGU4428801.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(20131..20661)
                     /gene="ppa"
                     /locus_tag="ACHKYO_RS09725"
                     /old_locus_tag="pgaptmp_001945"
     CDS             complement(20131..20661)
                     /gene="ppa"
                     /locus_tag="ACHKYO_RS09725"
                     /old_locus_tag="pgaptmp_001945"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HGU4428802.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            20971..21927
                     /gene="ytfQ"
                     /locus_tag="ACHKYO_RS09730"
                     /old_locus_tag="pgaptmp_001946"
     CDS             20971..21927
                     /gene="ytfQ"
                     /locus_tag="ACHKYO_RS09730"
                     /old_locus_tag="pgaptmp_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HGU4428803.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            22067..23569
                     /gene="ytfR"
                     /locus_tag="ACHKYO_RS09735"
                     /old_locus_tag="pgaptmp_001947"
     CDS             22067..23569
                     /gene="ytfR"
                     /locus_tag="ACHKYO_RS09735"
                     /old_locus_tag="pgaptmp_001947"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HGU4428804.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            23583..24605
                     /gene="ytfT"
                     /locus_tag="ACHKYO_RS09740"
                     /old_locus_tag="pgaptmp_001948"
     CDS             23583..24605
                     /gene="ytfT"
                     /locus_tag="ACHKYO_RS09740"
                     /old_locus_tag="pgaptmp_001948"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HGU4428805.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            24592..25587
                     /gene="yjfF"
                     /locus_tag="ACHKYO_RS09745"
                     /old_locus_tag="pgaptmp_001949"
     CDS             24592..25587
                     /gene="yjfF"
                     /locus_tag="ACHKYO_RS09745"
                     /old_locus_tag="pgaptmp_001949"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HGU4428806.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(25620..26618)
                     /gene="fbp"
                     /locus_tag="ACHKYO_RS09750"
                     /old_locus_tag="pgaptmp_001950"
     CDS             complement(25620..26618)
                     /gene="fbp"
                     /locus_tag="ACHKYO_RS09750"
                     /old_locus_tag="pgaptmp_001950"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HGU4428807.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            26794..28167
                     /gene="mpl"
                     /locus_tag="ACHKYO_RS09755"
                     /old_locus_tag="pgaptmp_001951"
     CDS             26794..28167
                     /gene="mpl"
                     /locus_tag="ACHKYO_RS09755"
                     /old_locus_tag="pgaptmp_001951"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HGU4428808.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGHGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPTHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(28323..28874)
                     /gene="yjgA"
                     /locus_tag="ACHKYO_RS09760"
                     /old_locus_tag="pgaptmp_001952"
     CDS             complement(28323..28874)
                     /gene="yjgA"
                     /locus_tag="ACHKYO_RS09760"
                     /old_locus_tag="pgaptmp_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HGU4428809.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            28968..30320
                     /gene="pmbA"
                     /locus_tag="ACHKYO_RS09765"
                     /old_locus_tag="pgaptmp_001953"
     CDS             28968..30320
                     /gene="pmbA"
                     /locus_tag="ACHKYO_RS09765"
                     /old_locus_tag="pgaptmp_001953"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HGU4428810.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            30503..30889
                     /gene="cybC"
                     /locus_tag="ACHKYO_RS09770"
                     /old_locus_tag="pgaptmp_001954"
     CDS             30503..30889
                     /gene="cybC"
                     /locus_tag="ACHKYO_RS09770"
                     /old_locus_tag="pgaptmp_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU4428811.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(30934..31398)
                     /gene="nrdG"
                     /locus_tag="ACHKYO_RS09775"
                     /old_locus_tag="pgaptmp_001955"
     CDS             complement(30934..31398)
                     /gene="nrdG"
                     /locus_tag="ACHKYO_RS09775"
                     /old_locus_tag="pgaptmp_001955"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HGU4428812.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(31556..33694)
                     /gene="nrdD"
                     /locus_tag="ACHKYO_RS09780"
                     /old_locus_tag="pgaptmp_001956"
     CDS             complement(31556..33694)
                     /gene="nrdD"
                     /locus_tag="ACHKYO_RS09780"
                     /old_locus_tag="pgaptmp_001956"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGU4428813.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(34088..35743)
                     /gene="treC"
                     /locus_tag="ACHKYO_RS09785"
                     /old_locus_tag="pgaptmp_001957"
     CDS             complement(34088..35743)
                     /gene="treC"
                     /locus_tag="ACHKYO_RS09785"
                     /old_locus_tag="pgaptmp_001957"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGU4428814.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(35777..37198)
                     /gene="treB"
                     /locus_tag="ACHKYO_RS09790"
                     /old_locus_tag="pgaptmp_001958"
     CDS             complement(35777..37198)
                     /gene="treB"
                     /locus_tag="ACHKYO_RS09790"
                     /old_locus_tag="pgaptmp_001958"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HGU4428815.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(37317..38264)
                     /gene="treR"
                     /locus_tag="ACHKYO_RS09795"
                     /old_locus_tag="pgaptmp_001959"
     CDS             complement(37317..38264)
                     /gene="treR"
                     /locus_tag="ACHKYO_RS09795"
                     /old_locus_tag="pgaptmp_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HGU4428816.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            38449..38502
                     /gene="mgtL"
                     /locus_tag="ACHKYO_RS09800"
                     /old_locus_tag="pgaptmp_001960"
     CDS             38449..38502
                     /gene="mgtL"
                     /locus_tag="ACHKYO_RS09800"
                     /old_locus_tag="pgaptmp_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HGU4428817.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            38643..41339
                     /gene="mgtA"
                     /locus_tag="ACHKYO_RS09805"
                     /old_locus_tag="pgaptmp_001961"
     CDS             38643..41339
                     /gene="mgtA"
                     /locus_tag="ACHKYO_RS09805"
                     /old_locus_tag="pgaptmp_001961"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGU4428818.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
BASE COUNT         9763 a        10477 c        11196 g        10016 t
ORIGIN      
        1 gtttttaaaa attgtgcata gtgaaaatca aagtaaaggt gtgcattatg caaaatcaca
       61 gcgtctctta gaaattaacc atgcgcactt gcagttaatg gaatccttat tggatgaagg
      121 gaagaaacac aatattttta agccagatat cgacccattg caggtgaata ttaatattgc
      181 tgcgcttggg ggatattatt tgatcaacca gcatacgctt ggcctggttt atcatatcag
      241 tatggtttcc ccccaggcac tggaagccag acgtaaggtc atcaaagaga caatccttag
      301 ctggcttttg gttgaccctt catctaccgc gcacgaataa aactgccatc cggctggcgg
      361 gtaaacagga cctgttgatt attccccgta tcaatggtta agcccgtcac cacgccgctg
      421 gcgttctggc ggattttcac catttgcccg ttttgcaaat tactcagcgg cttacccgcg
      481 ccttctacct gcgccattgc atagacatcg gtagcgggca gtccgtggtc gcggaacagc
      541 tgcgccatcg ttttaccggg ttcgacacgg taagagcgcc attgattatc aattccgcta
      601 tctggctgga atggttgcgt ttgggtagtt tgcggctgct cttctggctg gccttcctgt
      661 ataggttctg gagcgaccgg agccacctga tcaggatcat tttgtggggt gactaattgc
      721 gcccgcagct gttcttctgt cggcggttgt gattgcgact gaatatctaa ctgcgcttca
      781 cgcgtcacca ccggcgcgtt gggcgtatca tcagatggaa gcagaaatcc gaccaccaat
      841 acaatggcgg caataatgat cccccgacga tgcatcggcg gcagcgggtc catgaagcga
      901 aaattgtccg gcgcgtgcca gactttctcc agggttggtt ttagttcaaa gcgcccgggc
      961 atggtttcct cctgctccgc gtcttgttcc tcaatcatag cctatgaata agctaacgct
     1021 atgatgtccg tggtaaaccc gcctttatta tattcatacg ggatattgct attgtttctt
     1081 tttccccggg atttgtcatc attcctgcga caaagtttta cccaaagaag tgtggctgat
     1141 atgctgcccg ctacttttat accctaagaa aggaaatacg atgaccaccc caacttttga
     1201 caccatcgaa gcgcaagcaa gctacggcat tggtttgcag gtagggcaac aactgagtga
     1261 atctggcctg gaagggctgc tgccagaagc actggttgca ggtattgccg atgcgctgga
     1321 aggcaaacat ccggctgttc cggttgatgt ggtgcatcgc gcgctgcgtg aaatccacga
     1381 gcgcgccgat gccgttcgtc gtcagcgttt ccaggcgatg gctgctgaag gtgtgaaata
     1441 cctggaagaa aacgccaaaa aagaaggtgt gaatagcacc gaatctggcc tgcaattccg
     1501 cgtgatcaac cagggtgaag gcgcaattcc ggcacgtacc gaccgcgttc gtgttcatta
     1561 caccggtaaa ctgatcgacg gcaccgtgtt tgacagctcc gttgctcgtg gtgaacccgc
     1621 tgaatttccg gttaatggcg tgatccctgg ctggattgaa gcactgactc tgatgccggt
     1681 aggttctaaa tgggaactga ctatcccgca ggaactggca tatggcgagc gcggcgcagg
     1741 cgcatccatc cctccgttca gcaccctggt gtttgaagtc gaactgctgg aaatcctcta
     1801 agcagcgcat tctgttcccc tcgaaccaga ggggagcagg cattcagcaa taaacccttc
     1861 agtttgccaa acggcgctat tttgtgttgc aaagaccccg taagcgtgta tttttgtgag
     1921 ctgtttcgcg ttatcaccgt gatatgacac tcactttaaa cataaaatta acattagatc
     1981 taaatcttag tattcatccc gcgtattgtt acctaatatc gatgagtccc gatacagatt
     2041 cgtcgtatca tagactgact aaaggccgta gagcctgaac aacacagaca ggtacaggaa
     2101 gaaaaaaaca tggtagatca ggtaaaagtc gttgccgatg atcaggctcc ggctgaacag
     2161 tcgctacggc gcaatctcac aaaccgacat attcagctta ttgccattgg cggtgccatt
     2221 ggtacggggt tgtttatggg gtctggcaaa acgattagcc ttgccgggcc gtcgatcatt
     2281 ttcgtttata tgatcattgg ttttatgctc tttttcgtga tgcgggcaat gggggaattg
     2341 ctgctttcga atctggaata caaatctttt agtgacttcg cttccgattt actcgggccg
     2401 tgggcaggat atttcactgg ctggacttac tggttctgct gggttgtaac cggtatggca
     2461 gacgtggtgg cgatcacggc ttatgctcag ttctggttcc ccgatctctc cgactgggtc
     2521 gcctcgctgg cggtgatagt gctgctgctg acgctcaacc ttgccaccgt gaaaatgttc
     2581 ggtgagatgg agttctggtt tgcgatgatc aaaatcgtcg ccatcgtgtc gctgattgtc
     2641 gtcggcctgg tcatggtggc gatgcacttt cagtcaccga ccggtgtgga agcgtcattc
     2701 gcgcatttgt ggaatgacgg cggctggttc ccgaaaggtt taagtggctt ctttgccgga
     2761 ttccagatag cggttttcgc tttcgtgggg attgagctgg taggtacaac agctgcggaa
     2821 accaaagatc cggagaaatc actgccacgc gcgattaact ccattccgat ccgtatcatt
     2881 atgttctacg tcttcgcgct gattgtgatt atgtccgtga cgccgtggag ttcggtagtc
     2941 ccggagaaga gcccgtttgt tgaactgttt gtgttggtag gtttgcctgc ggctgccagc
     3001 gtgatcaact ttgtggtgct gacctctgcg gcgtcttccg ctaacagcgg cgtcttctct
     3061 accagccgta tgctgtttgg tctggcgcag gaaggtgtgg caccgaaagc gttcgctaaa
     3121 ctttctaagc gcgcagtacc cgcgaaaggg ttgaccttct cgtgtatctg tctgctcggc
     3181 ggcgtggtga tgctgtatgt gaatcccagc gtgattggcg cgttcacgat gatcacgacc
     3241 gtttccgcga ttctgtttat gttcgtctgg acgattatcc tttgctcgta cctggtgtac
     3301 cgcaaacagc gtcctcatct gcatgagaag tcgatctata agatgccgct cggcaagctg
     3361 atgtgctggg tatgtatggc gttctttgtg ttcgtggtcg tgttgctgac actggaagat
     3421 gacactcgcc aggcgctgtt ggtcaccccg ctgtggttta tcgcactggg gctgggctgg
     3481 ttgtttattg gtaagaagcg ggctgctgaa ctgcggaaat aaccgcatta tcatgctgga
     3541 tggcgcaatg ccatccagct tttagattac tcacccgcca gcgcgcgtgg gaacagtaca
     3601 ttgttttcca ggctgatgtg atccatcagg tcatcaatca gttcattaat gccgttatac
     3661 atcgctttcc aggtggtgca ggcttctggc ggcggtgtga cgttattggt agtgtgtttg
     3721 atcacttcca gcagttcgcc cgcttcatcg tgctcgcttt ccattacgct gattggcccc
     3781 attgcctggc tgcccatgcc ttgtttgatc atcgggaaga ggatctgctc ttctttcatc
     3841 atgtggctgg aaagctcttc atgcagcata gtcaggtatt ttgtcagccc ttttggcacg
     3901 ctcggtttgt cggcgtgaac gcgctcgact ttagtcgctt gcaggatcag ctccggcagc
     3961 tgctcgcggt gacgatcgtg gtagcgcacg atgatatggt caatgatttc tgccagcggg
     4021 gcgctacgcc agtctttctc aatcggttgt tcagcaagct ttgccagttc agcttcaatg
     4081 acctcaacat ccagttcttt acgtgccgcc gcgcgcgcca gcgtctgctt accgccacag
     4141 cagtaatcca tatcatattt acgaaacaga gccgaagcgc gagggataga gagcgccagt
     4201 tcacctaaag gttggtcgcg ataagccata gctgatacct cattcttaat aatataagat
     4261 gtattttaaa tgcatcttta aagcaaaagc tataaccctt acgtagcaga ggcttattta
     4321 actcactgcc agcgtgtcaa ctttctgaga cagattcagt taaaggtttt tctggttttg
     4381 ctctgaccgc aatgaccacg ccaatcacta acagcgcgat accgctcagc gtcattagcg
     4441 gcggcatttg ctggcggagt aaaaaggtgt acagcaaacc tgccagcgtt tcgaaaacaa
     4501 tcagcggccc gagaatcact gtcggtaatc gctggctggc gacgttccag cagagtgcgc
     4561 caacccatga gcaaagcacg gctatcgcaa ccatcagact aataaacacc agcggacggg
     4621 ggccaaaagg taaggagaag tccggcgttt gtatattcag ccagtaacag gcgacgagat
     4681 agccgatgag agaaaccggc agcgtgacca gcgcctgcgc cgtcgcccac atcatcggat
     4741 atttgtcggg attttcccgc agccagcggg cgttgcgcag ggcataccat gcccagcaga
     4801 ccacggaaac taacgctagc acgatgccag aggtataacg tgcccagtca aaatcgggga
     4861 gtccgtggtt taactcagca atattcacac tcgccaggcc gatgccaata caaatcagtg
     4921 ccggggcgag ttttccccac gcgagtttgc cgtcgcgctg gctataaagc agattggcaa
     4981 agacaggaat gaccaccggc agggtgccga taatcatcgt ggaaacaggc gcgccagtac
     5041 gttgaatggc actggcaagg cagaagtaat aaatgaggtt gcccatcata gtgagcatca
     5101 aggcggtaag ccagtcccga cgcgccaact gacgcagacg cacgcgtccc agccaggcaa
     5161 tgggcagcgc aattaaccct aacgccagat aacgccccat cgactgcaac atcgccgggt
     5221 attccggtac gatcaacggc ccgacaaaaa taagccccca catcaaccct gctaacaggg
     5281 cgtacagcac gccgctaatc attactggca tccattggtc tgtcagaaga aagttcagga
     5341 agcagacatg attgctcccg gcatcccgtc attattagag aggatgcggg aggatgccac
     5401 tttaattaac tagttattaa cattaaaaag atggcttacg ctttcggcta acgttgtcgt
     5461 tgggcggcca atcaatttgc taagcgtttt gctgttatca aacagaccac ctttcgatgc
     5521 gccaacgtca gaatccgcca gcatatccgc cagtccgtcg ggcagtccga cgcttttcag
     5581 tgccgcggcg aaatcggctt cgctcagatt ttgataggta acctgtttgc cgctctgttt
     5641 ggtcagctct gccgctaact gtgtcaatgt ccaggcacta tcgcccgcca gttcgtaaac
     5701 cttgccttcg tgaccggctt cgctaatcac gcgtgccgca gctgccgcat aatctgcccg
     5761 cgttgctgag gcgattttgc catcgcccgc cgcaccgata aatacgccgt gttccagtgc
     5821 tgccggggcg ctggcgaggt agttttcgct gtaccagccg ttgcgcagca gggtgtaaac
     5881 gatgccagaa tcagccaaca ttttctccgt ctcgatgtgc tcatcggcga ggccgagcgg
     5941 ggaggtatct gcatgtagca ggctggtata agcgataaat ttcacaccag ccgcctttgc
     6001 ggcattaata acattacgat gctgcggggc acgttgaccc acttcgctgg aagagatcag
     6061 cagtagtttt tccactccct gaagtgcaga tgtcagtgcg gcttcatcgc cgtagtcagc
     6121 ctgacgcacg gtaatgcctt gtgctgccag agcctgggct tttgccggat tacgaacgat
     6181 agccactatt tggctgacag gaaccgtttt cttcaaggat tcaataacat agtgaccaag
     6241 ttggccagtg gcaccagtaa tagcgatcat gggaagtctc catcgttttg tcttgtgttt
     6301 aaatacgcta acacctaaac ttacttttag taagtacgca caaaaaggta agtatgaaat
     6361 gagtcaggtt agcctgtcgc aacaactgaa agagggtaat ctctttgcgg aacagtgccc
     6421 gtcgcgcgag gtgttgaaac acgtcaccag ccgttggggg gtgttgattc tggtggcgct
     6481 acgcgaaggt actcatcgct ttagcgacct gcggcgcaaa atcggcgggg tgagcgaaaa
     6541 gatgcttgcg cagtcgttac aggcgttaga acaggatggt tttcttaacc gtatcgcgta
     6601 tccggtggtg ccgccgcatg tggaatatag cctcacgccg ctgggcgagc aggtgagcga
     6661 aaaggttgct gcactggcgg actggattga gttgaatttg cccgaggtgt tggcggtgcg
     6721 ggatgaacgt gcggcataac tgccggatgc gctgagctta tctggtctac ggtgcatggc
     6781 ttgtagggcg gataagatgc gccagcatcg catccgccaa taaatgccgg atgcggttga
     6841 tgatttatag gccgaaaaag aagtgtttta cttactcaaa tccacctgat aaatcgcaaa
     6901 cccgatatca tcggtcgcga ctttattcat cggatattgc cctttctctt taataaacgc
     6961 tgcggcttta tctgacggag aagtttcgaa acggatatcc agtttcttat cgccagctat
     7021 cggtgctaaa cgccagttgt tatctgccgc cgggtgaatt tcccccgcac gtttcgactc
     7081 atcagcaatc cacgctgcca gcaccgagcg gttctcatcc ggtgaagcaa aagcgatatg
     7141 gctgtcgccc gtaccggcaa atttgccgcc gtaagcgcga tagttattgg tggcaacgag
     7201 gaacatggcg ttcggatcaa tcggcttgcc attaaaggtc aggttcttaa tcctttccgc
     7261 attggcatta atcatctggc actcgccgtc ataacgggca ggctgggtaa catcaatctg
     7321 ataattcaca ccatcaatca catcaaagtt ataggtgcgg aaaccatccc agttgatgag
     7381 tgattgcggt ttcgtgctgt taggatcaat ctggttaaac tgtccggcgg agcactccag
     7441 ccactctttc acctctttac cgctggcttt cactacaatc agcgtattgg ggtagagata
     7501 aagatcggcg gcattacgga aggtcaactg gcctttttcc acctccacat agcttgccgg
     7561 gtcatttttg cgaccaccaa ctttaaacgg tgcggcagct gaaagcaccg gcagttttgc
     7621 cagatccgga tcgccctgaa tgtaatgttc gacatacgct ttttgcgcgt tgttcactac
     7681 ttgcacggtc ggatcgtcct gcaccagcgc cagatagcta tacatattgt cggcagattt
     7741 accgattggc ttgctgacga actggcgtgt ggcatcgtga tcggctttga gtgtttctac
     7801 cagcttgctg tcttccgccg tgagggattt tttattggcg atgtcgtaaa tcggccgagc
     7861 ttccgctttc gcctgcgtca cctgccattt accgctgtca ttactgagtt gtaagtcgac
     7921 caccccaaga tgatcgcccc acatgcccgg cattaccgcc ggaacaccat tcagcgtgcc
     7981 tttggtgata tcagcccctt cgatatcagc aaaatcttta cccgggaaaa cggcgtgagc
     8041 atgaccaaac ataatggcgt taacgcccgg aatttcactg aggtaataaa ctgagttttc
     8101 cgccatcact ttatacggat cggcagacag cccggaatgt gccagaacga caacgacatc
     8161 ggcacctttc tcgcgcattt caggcacgta tttgcgcacg gtttcggtaa tatcattcac
     8221 cgtcactttc ccggataaat tagctttatc ccagcccatg atttgcggcg gtacgacgcc
     8281 aatatagcca atcttcagcg tctgtttttt tccgtcttta tcgaccactt cggtgtcttt
     8341 aattaaatac ggtgtaaaca ttggctgttt ggttctggcg tcaatgacgt tggcatttac
     8401 ataagggaat ttcgctcccg ccagcgcatt tttcaggtaa tccagaccgt agttaaactc
     8461 atggttgcca agcgtgccga cggtatagtc cagcgtattt aatgccttat acactgggtg
     8521 aatatcacct gcttttaatc ctttcgccga catgtaatcg gccagcggac tcccctgaat
     8581 caaatcgccg ttatcgacca gtacgctgtt tttcacttca ttgcgggcat cgttaatcag
     8641 gcttgccgta cgtaccagtc cgaatttttc cgtggcggtg tctttgtaat aatcgaaatc
     8701 catcatgttg ctatgcagat cagtggtttc catgatacgc agatcgaccg tcgctgcatt
     8761 cacactggcg gtaatcagcg tggccaggag cgttgcgcta aacttaatca tcagggacat
     8821 ccttttatca tcgggaatac gaaagaaaag ggagaataaa cgtcttactt atagaacagt
     8881 gaagaatgcc acaattttac gctttgaaaa tgatgacact atcacagttg gcgcattcat
     8941 taacgatagg gtataagtaa aacaataagt taacaccgct cacagagacg aggtggagaa
     9001 atgttagatc aagtatgcca gcttgcacgg aatgcaggcg atgccattat gcaggtctac
     9061 gacgggacga aaccgatgga cgtcgtcagc aaagcggaca attctccggt aacggcagcg
     9121 gatattgccg ctcacaccgt tatcatggac ggtttacgta cgctgacacc ggatattccg
     9181 gtcctttctg aagaagatcc tcccggttgg gaagtccgtc agcactggca gcgttactgg
     9241 ctggtagacc cgctggatgg tactaaagag tttattaaac gcaatggcga attcaccgtt
     9301 aacattgcgc tcattgacca tggcaaaccg attttaggtg tggtgtatgc gccggtaatg
     9361 aacgtaatgt acagcgcggc agaaggcaag gcctggaaag aagagtgcgg tgtgcgcaag
     9421 cagattcagg tccgcgatgc gcgcccgccg ctggtggtta tcagccgttc ccatgcagat
     9481 gcggagctga aagagtatct gcaacagctt ggcgaacatc agaccacgtc catcggctct
     9541 tcgctgaaat tctgcctggt ggcggaagga caggcgcagc tgtacccgcg cttcggacca
     9601 acgaatattt gggacaccgc cgctggacat gctgtggccg cagctgccgg agcgcacgtt
     9661 cacgactggc agggtaaacc gctggattac actccgcgcg agtcgttcct gaatcctggg
     9721 ttcagagtgt ctatttatta attaatttat gcagcaggtc catcacttgc tgcacctctt
     9781 cctgagtaag cgccccgtct ttggcccatt gcacgcgacc gtctttattc agcaccacta
     9841 cagcggaact tttttcatcc agctgccagg caccgcgtgc gacgccattg ctatcaacaa
     9901 taaactgcga ccagggataa agctttttat tactctccag actgctgcgc acaaacatgc
     9961 cggaacccgg aattgcgtcg tcggtgttaa caatggtggt ggtctggtaa cgatcgtgcg
    10021 gtaacttcgc tgatttaatc gcttcaatca gcgtcgcgtt tttctctttt gcagaggtgc
    10081 gaccagcaat atgttgcagt actcgcactt ttcccactaa ctgcgcgctg ttccaggttt
    10141 tgtagctaaa ctgatcttta tcaagcacca actcgccacg atcggtgatg ccaatcggcg
    10201 gcactcgctg accggtttcg aactgatgtg cggaagccat catcggcaac agcaggcagg
    10261 tgagtgccag aatcttgcgt agggtcatgg tgtttccttc ttatgatatg caggtgatcc
    10321 gaccacttgg gtctgagttt taatcatatg tgctatttat cgaatttccc gcaagtgtga
    10381 tgccagtttg cggtcaagcg cacaaatcat atgaaaaatg aatgcttata ctgaagaccg
    10441 cgcttcggta aaaagataat tctgaataat tgtaaccttt aggtaaaaaa agttatacgc
    10501 ggtggaaaca ttgcccggat agtctatagt cactaggcat taaaatttgc gcctcataat
    10561 agttgggccg attgtggcac cgcacaggcg taatactcag caggagataa caatgaaaat
    10621 tttccaacgg tacaacccac ttcaggtggc gaagtacgta aagatcctgt tccgtggacg
    10681 gttatacatc aaggacgttg gcgcttttga atttgataag ggtaagattc ttatcccaaa
    10741 agtgaaggat aagctgcatt tgtcagtgat gtccgaagtt aaccgtcagg ttatgcgtct
    10801 gcaaacagag atggcttaac caaagtgcta tgcagtaata agacggctcc tgattcagga
    10861 gccgttgatg tttctggggg ttacgcgacg ctttcttctt tatctttagc gtcaggcagt
    10921 tttggcgaaa gggcggtggc cttgctgtcg atccgggtca ccagcagctg gtcgatgcgg
    10981 tagttatcga tatccaccac ttcaaatttg tagccggcga atttcaccga atcggtgcgt
    11041 ttcgggatct tacgcagcat aaacatcata aagcctccga tggtttcgta gttgcccgac
    11101 tgcgggaact cgtcaatatc cagcacgcgc atgacgtcgt caattggggt gccgccgtca
    11161 atcagccatg agttctcatc acgggcgaca atctgctctt ccagcccctg accgaccaga
    11221 tcgcccatca gcgtggtcat cacgtcgttg agggtgatga tccccaccac cagcgcgtac
    11281 tcgttcatga tcaccgcgaa gtcttcacct gcggttttaa aactttccaa cgcctctgaa
    11341 agggttaacg tatccggcac aatcagcgtg ttgcgaattt gtacgccgct gttcagtgcc
    11401 aggctttggt tagccagcac gcggttcagc aggtctttag aatcgacata accgatgatg
    11461 tggtcaatat cttcattaca gacgaggaac ttagagtgcg gatgttccgc caccttattc
    11521 ttcaggcttt gctcatcttc gtggagatca aaccaaatca cgttttcacg cggtgtcatt
    11581 gaagacggaa cggtacggga ttccagctca aagacgtttt caatcagctc gtgttcctgt
    11641 ttacgtaaca cgcccgccag cgcaccggct tccactaccg cgtagatgtc atcagaagtg
    11701 atgtcatctt tacgtaccat tggcagtttg aaaatacgga agatcatgtt cgccaggccg
    11761 ttgaagaacc acaccagcgg ggtgcaaacg tacaggcaga agcgcatcgg gttgatgata
    11821 cgcaaagcca ccgcttctgg cgcaatcata ccgatgcgtt tcggggttaa atccgcaaac
    11881 aggataaaca tgccagtcac taacgagaaa gagagaataa agctcagttg ctcagagagt
    11941 tctgccgaca tatagcggga gaacaggctg tgaaaagctg gagaaaatgc cgcatcaccg
    12001 acgataccgc cgagaatcgc aactgcgttc agaccgattt ggaccaccgt aaagaacatg
    12061 ccgggatttt cctgcatatt cagaacgcgt tgggcattta tattgccttc atcagccagc
    12121 agtttaagtt tgattttgcg tgaggcggca agtgagatct cagacatcga gaagaacgca
    12181 cttacagcga tcaagcagag tatgactaaa atactgttta acatatctta tccgaccttt
    12241 caggccagat cctcggaagg ggaagttgat taatttgtgt gaaatacaca ttgaaagccg
    12301 attcgaagaa agtgaaccgg cattttcagt ggctagtata gcgtaaggta ctgtaaagcc
    12361 gccagagggt taaaattcag acagctgaaa aatgcaaaac tgcctgatgc gctatgccga
    12421 tcaggcctac gcagtccttg caatatattg aatttggacg attttgtagg cctggcaagg
    12481 cgtttacgcc gcatccggca taaacaaaac gcactttatc aacaatctac atttataccc
    12541 gtaacgctat gcttccggcg gcagacagac gccaattccg ccaattccac agtagccata
    12601 cgggttttta tgcagatatt gctggtggtc atcctcggca taataaaacg gtgtggcgtt
    12661 agcgatttcc gtggtgatgc gacgatcgtc atcggcggca agcatcgccg cctgaaaacg
    12721 ttccagactg gcgcgagctg cggcatcctg ttctggggtc agcggataaa tcgctgaacg
    12781 atactgcgtg ccttggtcat tgccctgacg cattccctgg gcaggatcgt gattctccca
    12841 aaatacctgt agcaactgct catagctgat gacggaagga tcgtaaacaa tgcgtaccgc
    12901 ttcggcatga ccagtatcac cggagcacac ttcccgataa gtcggatttg gcgtatagcc
    12961 gccggtataa cctgcggcgg tgctgtaaac gccgggtaac tgccagaaca gacgctccac
    13021 accccagaaa caacccatcg caaaaatggc aatctccatt ccgtcaggta cattggtcat
    13081 tgagtgaccg ttgaccgcat gcagcgtggc tacgggcatc ggggtgttac gtccaggcag
    13141 ggcatcggcg ggggaaacaa gatgcttttt atcaaataaa ctcatggtgt cgctctcccg
    13201 aaaatcggtc attggggtta aggttgtaac aagaggcgta tttgcacaca ataaccaccg
    13261 tgaataggtc taaagtaaaa cataagaaat atttggggtt tagtctgctt tttaatccat
    13321 attactggat ttttgttaag ccgtttaacg gcgttccagg ggcaggaaaa aaggatattc
    13381 aggagaaaat gtgcgctata tccgacagtt atgctgtgta agcttactct gcttaagcgg
    13441 atctgccgtc gccgcgaacg tccgtctaca ggtcgagggg ttatcgggac agctggaaaa
    13501 gaacgttcgt gcgcagcttt ctacgattga aagtgatgaa gtgacgccag accgtcgctt
    13561 tcgcgcacgc gtcgatgatg ccatccgcga aggtctgaaa gcgctgggtt attaccagcc
    13621 gaccattgaa tttgatctcc gtccaccgcc aaagaaaggg cggcaggtat tgatcgccaa
    13681 agttacgcca ggcgtgccgg tgttaattgg cggcaccgat gtggtattgc gcggcggcgc
    13741 gcggaccgat aaagactatt tgaaattgct cgatactcgc ccggctattg gcacggtact
    13801 gaaccagggc gattatgaaa atttcaaaaa gtccttaacc agcattgcgt tgcgtaaagg
    13861 ttatttcgat agcgaattta ccaaagcgca gctgggcatt gcgctcggcc tgcataaagc
    13921 cttctgggat attgattata acagtggcga acgttaccgc tttgggcatg tgacctttga
    13981 aggatcacaa atccgcgatg aatacctgca aaatctggtg ccgtttaaag agggcgatga
    14041 gtacgaatcg aaagatctgg cggaactgaa ccgccgactt tctgctaccg gctggtttaa
    14101 ctcggtggtg gtggctccac aatttgataa agcgcgcgaa acgaaagtat tacccttgac
    14161 gggcgtggtt tcgccgcgaa ctgaaaacac catcgaaacc ggggtcggtt actctacgga
    14221 cgtgggaccg cgcgtgaaag cgacgtggaa aaagccgtgg atgaactctt atggtcacag
    14281 tctgaccacc agtaccagta tttccgcgcc ggaacagacc ctcgacttca gctataaaat
    14341 gccgctgctg aagaatccac tggaacaata ttatttggtg cagggcggtt ttaagcgcac
    14401 tgacctgaac gataccgaat ctgactccac tacgctggtg gcttctcgct actgggatct
    14461 ctccagcggc tggcagcgtg ccattaacct gcgctggagc ctcgaccact ttacccaggg
    14521 tgaaattacc aacaccacga tgctgtttta tcctggggtg atgattagcc gcacgcgttc
    14581 tcgtggtggc ctgatgccaa cctggggcga ctcgcaacgc tactctatcg actactccaa
    14641 cacggcctgg ggctcagatg tcgatttctc cgttttccag gcgcagaacg tctggatccg
    14701 cacactgtac gatcgccatc gttttgtgac acgcggcacg ctgggctgga ttgaaacggg
    14761 tgatttcgac aaagtaccgc cggatctgcg tttcttcgcc gggggcgatc gcagtattcg
    14821 cggctacaaa tacaaatcta tcgccccgaa atacgccaac ggtgacctga aaggggcctc
    14881 gaagctgata accggatcgc tggaatacca gtacaacgtg accggaaaat ggtggggcgc
    14941 ggtgtttgtc gatagtggcg aagcggtaag cgatattcgc cgcagcgact ttaaaaccgg
    15001 taccggggtc ggcgtacgct gggaatcgcc ggtcgggcca atcaaactcg attttgccgt
    15061 accggtcgcg gataaagacg agcacgggtt acagttttac atcggtctgg ggccagaatt
    15121 atgagtttat ggaaaaaaat cagcctcggc gtggttatcg ttatcttact gttgctggga
    15181 tcggtggcgt ttctggtggg caccaccagc ggcctgcatc ttgtatttaa agcggcggat
    15241 cgctgggtgc caggactgga tattggcaag gtcaccggcg gctggcgcga tctcaccttg
    15301 tctgacgttc gttatgagca gccaggcgtg gcggtaaaag cgggtaatct gcatctggcg
    15361 gtcggtcttg agtgcctgtg gaacagtagc gtttgtatta atgacctggc gctgaaagac
    15421 attcaggtca acatcgacag taaaaaaatg cccccttctg aacaggttga agaagaggaa
    15481 gatagcggtc cgctggatct ctccacgccg tatcccatca ccctgacacg ggtggcgctg
    15541 gataacgtca acatcaagat tgatgacacc acggtatcgg tgatggactt cacctccggc
    15601 ctgaactggc aggagaaaac cctgaccctg aaaccgacgt cgctgaaagg cctgttgatt
    15661 gccctgccga aagtggcgga agtggcgcag gaagaagtgg tcgaaccgaa aattgaaaat
    15721 ccgcagccgg atgataagcc gctcggcgaa acgttgaaag atctcttttc tcgtccggta
    15781 ttgccggaaa tgaccgacgt gcatttgccg cttaacctga acattgaaga gtttaagggc
    15841 gagcagctgc gcgtgacggg cgacacggac atcaccgtga acaccatgct gctgaaagta
    15901 agcagcatcg acggtaatac taaactggac gccctggata tcgattccaa tcaagggatc
    15961 gtcaacgcca gcggcacggc acagctgtca gacaactggc cggtggatat caccctcaac
    16021 agcacactaa acgtggagcc gttgaaaggt gaaaaagtga agctgaaagt gggcggcgcg
    16081 ctgcgcgaac agctggagat tggcgttaac ctttccggtc cggtggatat ggatttacgc
    16141 gcccagacgc gactggcgga agctggattg ccgctcaacg tggaagtgaa cagcaaacag
    16201 ctttactggc cgttcactgg tgagaagcag tatcaggcgg atgatctgaa actgaaactt
    16261 accggcaaaa tgaccgatta cacgctctct atgcgtacgg cagtgaaggg acaggagatc
    16321 ccgcccgcga ctattaccct cgacgcgaaa ggtaatgaac agcaggtcaa tctcgacaaa
    16381 ctcaccgttg cggcgctgga aggcaaaact gaactcaagg cgttgctcga ctggcaacag
    16441 gccattagtt ggcgcggtga gctaacgctt aatggcatta acaccgccaa agagttcccg
    16501 gagtggccgt cgaaactcaa tggcttgatt aaaacccgcg gtagcctgta cggcggcacc
    16561 tggcagatgg aggtgccaga actgaagctg accggtaacg tcaaacagaa caaagtgaac
    16621 gttgacggca cgctgaaagg caacagttat atgcagtgga tgatcccagg gcttcatctg
    16681 gaactggggc caaacagtgc cgaagtgaaa ggcgagctgg gggtaaaaga tctcaatctt
    16741 gatgccacca tcaacgcgcc ggggctggat aacgcgctgc cggggcttgg cggtacagcg
    16801 aaagggctgg tgaaagtacg cggcacggtg gaagcgccac aactactggc agatatcacc
    16861 gcgcgcggcc tgcgctggca ggaactttcc gtggcgcagg ttcgcgtgga aggcgacatt
    16921 aaatccaccg atcagatcgc cgggaaactc gacgtacgcg ttgagcaaat ttcgcagccg
    16981 gatgtaaata tcaacctcgt caccctgaat gccaaaggca gcgaaaagca gcacgagcta
    17041 cagttgcgga ttcagggcga gcctgtctcc gggcagctta atctggcagg aagttttgat
    17101 cgcaaagaag aacgctggaa gggaactctt agcaataccc gcttccagac gccggtcggc
    17161 ccgtggtcgc tgacccgcga tattgcgctg gattaccgca ataaggagca aaaaatcagc
    17221 atcgggccac actgttggct taacccgaat gcggagctgt gcgtgccgca aactatcgat
    17281 gcgggggccg aagggcgtgc ggtggtgaat ctcaaccgct tcgacctcgc catgctgaaa
    17341 ccgtttatgc cagaaaccac tcaggccagc ggtatcttca cgggtaaagc ggatgttgcc
    17401 tgggacacca cgaaagaggg gctgccgcag ggcagtatca ccctttcggg gcgtaacgtg
    17461 caggtaacgc aaaccgtcaa cgatgcggcg ctgcccgtgg cgtttcagac gctgaatctg
    17521 acggcggaat tgcgtaacaa ccgtgccgaa ttgggctgga ccatccgcct gaccaataac
    17581 ggccagtttg atggacaggt gcaggtgacc gatccgcaag gccgccgtaa tcttggtggc
    17641 aacgtcaata tccgtaactt caaccttgcg atgataaacc ccatctttac ccgtggggaa
    17701 aaagcagcgg ggatggtgag tgccaacttg cgtctgggtg gtgatgtgca aagcccgcag
    17761 ttgtttggtc agcttcaggt tacgggtgtg gatatcgacg gcaactttat gccgtttgat
    17821 atgcaaccga gccagcttgc ggtcaacttt aacggtatgc gctcgacgct tgccggtaca
    17881 gtacggaccc agcagggtga aatctacctg aacggtgatg ccgactggag ccaaattgaa
    17941 aactggcggg cgcgggtaac ggcgaaaggc agtaaagtgc ggatcaccgt gccgccgatg
    18001 gtacgaatgg atgtatcgcc agatgttgta ttcgaggcta caccaaacct gtttaccctc
    18061 gatggtcgcg tggatgtccc ttgggcgcgc atcgtggtgc acgatctgcc ggaaagcgca
    18121 gtaggcgtct ccagcgatgt ggtgatgctt aacgataacc tgcaaccgga agagccgaaa
    18181 acggcgtcga ttccgattaa cagtaacctg attgtccacg ttggcaataa tgtgcgcatt
    18241 gacgcctttg gcctgaaagc gcggctgacg ggcgatctta acgtcgttca ggacaaacaa
    18301 gggctgggcc tgaacgggca gatcaacatc cctgaagggc gcttccatgc ctatggtcag
    18361 gatctgattg tgcgtaaggg tgagttactg ttctctggtc cgccggatca accgtatctt
    18421 aatattgaag ctattcgtaa cccggatgct acagaagacg acgtaatcgc cggagttcgc
    18481 gtcactggtc tggcggacga accgaaagcg gagatcttct ctgacccggc gatgtcgcaa
    18541 caagctgcat tgtcttattt gctacgtgga caagggctgg agaacgatca gagcgacagt
    18601 gcggcaatga cctcgatgct gattggtctg ggggttgcgc aaagtggcca gattgtgggt
    18661 aaaatcggcg agacgtttgg cgtaagcaat ttagcgctcg acacccaggg agtaggcgac
    18721 tcctcccagg tagtggtcag cggctatgta ttgccaggtc tgcaagtgaa atatggcgtg
    18781 ggtatatttg actctatagc aacactcacg ttacgttatc gcctgatgcc taagctatat
    18841 ctggaagccg tgtctggtgt agaccaggca ctggatttgc tctatcagtt cgagttttag
    18901 caatgcgaat atttgtctac ggcagtttac gccacaaaca aggcaacagt cactggatga
    18961 ccaatgccca gttactgggc gatttcagta tcgataacta ccagttgtat agcctgggcc
    19021 actatccagg cgcagttccg gggaacggaa cagtacacgg tgaagtttat cgtattgaca
    19081 acgccacgct ggccgaactt gatgccttgc gcaccagggg cggtgaatac gcgcgccagt
    19141 tgattcagac gccgtacggg agtgcatgga tgtacgttta tcaacgaccc gtcgatggat
    19201 taaagctaat tgaaagcggc gactggttag acagggataa gtaaccatat gcatacgcca
    19261 ccttcgggtg gcgttgtttt ttgcgagacg actcgcattc tgttttgtaa ttccctcacc
    19321 ctttgctttt ctctccgagc cgctttccat atctgttaac gcataaaaaa ctctgctggc
    19381 gttcacaaat gcgcaggggt aaaacgtttc ctgtagcacc gtgagttata ctttgtataa
    19441 cttaaggagg tgcagatgcg tattaccata aaaagatggg ggaacagtgc aggtatggtc
    19501 attcccaata tcgtaatgaa agaacttaac ttacagccgg ggcagagcgt ggaagcgcag
    19561 gtgagcaaca accaactgat tctgacaccc atctccaggc gctactcgct tgatgaactg
    19621 ctggcacagt gtgacatgaa cgccgcggaa cttagcgagc aggatgtctg gggtaaatcc
    19681 acccctgcgg gtgacgaaat atggtaaaga aaagtgaatt tgaacgggga gacattgtgc
    19741 tggttggctt tgatccagca agcggccatg aacagcaagg tgctggtcga cctgcgcttg
    19801 tgctctccgt tcaagccttt aatcaactgg gaatgacgct ggtggccccc attacgcagg
    19861 gcggaaattt tgcccgttat gccggattta gcgttccttt acattgcgaa gaaggcgatg
    19921 tgcacggcgt ggtgctggtg aatcaggtgc ggatgatgga tctacgcgcc cggctggcaa
    19981 agcgtattgg tctggctgcg gatgaggtgg tggaagaggc gttattacgc ttgcaggcgg
    20041 tggtggaata aggtgtgttt atttatcgct ggcataaaaa aacccttact aaccgaagct
    20101 cggcgttcag ggttattgcg ctagaagaac ttatttattc tttgcgcgct cgaaggaagc
    20161 aacgatttca gctttagcgg cttctgcgtt ttcccaacct tcaactttca cccacttgcc
    20221 tttttcgagg tctttgtagt gctcgaagaa gtgagcgatc tgcgctttca gcagctctgg
    20281 cagatcgtta acgtctttaa tgtgatcgta ttctttgctc agcttgctgt gcggaaccgc
    20341 aaccagtttc gcatcttcac cggcttcgtc ggtcattttc agaacgccaa ccggacggca
    20401 acggatcaca gaacccggct gcagcgggta cggagtcggg accagtacgt caaccgggtc
    20461 accgtccaga gacagggtgt ggttgatgta accgtagttg cacggataga acatcgcggt
    20521 ggacatgaag cggtcaacga acagtgcgcc gctctctttg tcgatttcgt atttgatcgg
    20581 atctgcgtta gccgggatct caataacaac gtagatgtct tccggcagat ctttacccgc
    20641 agggacgttg agtaagctca tgtctgtttc ctttaaaaat atgtggcaaa caagtgccga
    20701 gtattatagc caactcgcgc cgaatgtctt tgcttgtttt cgtcttcatt ttcctttttc
    20761 gccagtttta agacggtata cagagcagga aaatccataa ccgtagccgc atttttcata
    20821 gtgagatgaa agcgattaca aacttgtgat taacgtttta tttacttttt tgaagtgtga
    20881 tgtaacgcaa tccgttacat aacgaattgt ctatagtttt ttcgcgaaca tcttttcacc
    20941 aataataatt accccgacga ggacaaccct atgtggaaac gcttacttgt agtctctgca
    21001 gtctcggcag ccatgtcgtc tatggcgttg gccgctccat taaccgtagg attttcgcag
    21061 gtcggatcgg aatccggctg gcgcgctgca gaaaccaatg tagcgaaaag tgaggccgaa
    21121 aaacgcggaa ttacgttgaa aattgctgat ggtcagcaaa agcaggaaaa ccagattaaa
    21181 gcggtacgtt ccttcgttgc acaaggggtg gatgcgatct ttatcgctcc ggtggtcgcg
    21241 acaggttggg aaccggtatt aaaagaggcg aaagatgccg aaatcccggt cttcttgctc
    21301 gaccgttcca tcgatgtgaa agacaaatct ctctatatga ccaccgttac tgccgacaac
    21361 atcctcgaag gtaagttgat cggtgactgg ctggtaaaag aagtgaatgg caaaccatgc
    21421 aacgtggtgg agctgcaggg caccgttggg gccagcgtcg ccattgaccg taagaaaggc
    21481 tttgccgaag ccattaagaa tgcgccaaat atcaaaatca tccgctcgca gtcaggtgac
    21541 ttcacccgca gtaaaggcaa agaagttatg gagagcttta tcaaagcgga aaacaacggc
    21601 aaaaacatct gcatggttta cgcccataac gatgacatgg tgattggcgc aattcaggca
    21661 attaaagaag cgggcctgaa accgggcaaa gatatcctca cgggttccat tgacggtgta
    21721 cccgatatct acaaagcgat gattgatggc gaagcgaacg ccagtgttga actgacgccg
    21781 aatatggcag gtcccgcctt cgacgcgctg gagaaataca aaaaagacgg caccatgcct
    21841 gaaaagctga cgctgaccaa atccaccctt tatctgcctg ataccgcaaa agaagagtta
    21901 gagaagaaga aaaatatggg gtattgaggg ttgctatgcc tgatgccgat tcgtaggccg
    21961 gataaggcgc tcgcgccgca tccggcggtg gtgcgctgaa gcctgatgcg acgcttaccg
    22021 cgtcttatca tgcctactgg gagcacgctt tacaccgggg gaaaccatga cgactaacca
    22081 acaccaggag atcctccgca ccgaaggatt aagtaaattt ttccccggcg tcaaagcgtt
    22141 agacaacgtt gatttcagcc tgcgccgtgg cgaaatcatg gcgctgctcg gtgaaaacgg
    22201 ggcgggaaaa tcgacgctaa tcaaagcatt aaccggtgta taccacgccg atcgcggcac
    22261 catctggctg gaaggccagg ctatctcacc gaaaaatacc gcccatgcac aacaactcgg
    22321 catcggaacc gtttatcagg aagtcaacct gctacccaat atgtcggtcg ctgataatct
    22381 attcataggt cgcgaaccca aacgtttcgg ctttctacgc cgcaaagaga tggaaaagcg
    22441 tgccaccgaa ctgatgacat cttacggttt ctccctcgac gtgcgtgaac cgcttaaccg
    22501 tttttcggtg gcgatgcagc aaatcgtcgc catttgccgg gctatcgatc tctccgccaa
    22561 agtgctgatc ctcgatgaac ccaccgccag cctcgacacc caggaagtag agttactgtt
    22621 tggcctgatg cgtcagttgc gcgatcgcgg cgtcagcctg atctttgtca ctcactttct
    22681 cgatcaggtc tatcaggtca gcgatcggat caccgtctta cgcaacggca gtttcgtagg
    22741 ctgtcgggaa acgcgcgagc taccgcagat cgaactggta aaaatgatgc tggggcgcga
    22801 gctggatacc cacgcgctac agcgtgccgg gcgaacattg ttgagcgaca aacccgttgc
    22861 cgcgttcaaa aattacggca aaaaaggaac gatcgcaccg tttgatctcg aagtacgccc
    22921 cggcgagatc gtcggcctgg caggcttgct gggatccgga cgtaccgaaa ccgccgaagt
    22981 gatcttcggt atcaaacctg ctgacagcgg cacggcgttg atcaaaggca aacagcaaaa
    23041 cctgcgatcg ccacatcagg cttcggtact gggcatcggc ttctgcccgg aagacaggaa
    23101 aaccgatggc atcatcgccg ccgcctcggt acgggaaaat atcatcctcg ctctccaggc
    23161 ccagcgcggc tggctacgac ccatttcccg caaagagcag caagagattg ccgaacgctt
    23221 tatccgccag cttggcattc gcacaccttc aactgaacaa ccgattgaat ttctctctgg
    23281 cggcaatcag caaaaagtgt tgctttcacg ttggctactg acccgaccgc aatttctgat
    23341 cctcgacgag ccaacccgcg gcattgatgt tggtgcccac gccgagatca tccgcctgat
    23401 tgaaacgctg tgtgctgacg gtctggcgct gttggtgatc tcctccgaac tggaagagct
    23461 ggtgggctat gccgatcggg tgattatcat gcgtgatcgc aaacaggtgg cggagatccc
    23521 gctggcagag ctttccgttc cggcgatcat gaacgccatt gcggcgtaag gagaacagtg
    23581 tgatgcctca atctctcccg gacactacgc cgccgaaaag gcgctttcgc tggccaacgg
    23641 gaatgccgca gctggcggcg ctattgctgg tgctgctggt cgatagcttg gtagctccgc
    23701 atttctggca ggtggtgctt caggatgggc gtttgttcgg tagccccata gacattctta
    23761 accgtgcagc acccgttgcg ttactggcga ttggtatgac gctggtgatc gccaccggtg
    23821 ggattgatct ctccgtaggg gcggtgatgg ctatcgccgg agccacaacg gctgcgatga
    23881 cggtcgcggg agtcagcctg ccgattgttt tgttaagcgc cctgggcaca ggcatcctgg
    23941 cgggattgtg gaacggcata ctggtagcga tcctcaaaat tcagccattt gtcgccaccc
    24001 tgatcctgat ggtcgccggg cgcggcgtgg cgcaactgat cacctccgga cagatcgtca
    24061 cgtttaactc gccggatctc tcatggtttg gcagtggatc gctgttgttc ctgccaacgc
    24121 cagtcattat tgcggtactg acgcttctcc tgttctggct gttgacccgc aaaacggcac
    24181 tggggatgtt tatcgaagcc gttggtatca acattcgggc ggcaaaaaat gccggggtaa
    24241 acacgcgaat catcgtcatg ctcacctacg tgttgagcgg gctatgtgcg gcgattgcgg
    24301 gcattatcgt ggcggcggat attcgcggtg ccgatgccaa caacgccgga ttatggctgg
    24361 agctggacgc cattctcgcg gtggtgattg gcggcggatc gctgatgggc gggcgcttta
    24421 acctgctgct ctcggtagtg ggggcgctga ttattcaggg gatgaacacc ggaattttgc
    24481 tttcgggctt tccaccggag atgaaccagg tggtgaaagc ggtggtggtg ctttgcgtgc
    24541 tgattgtcca gtcgcaacgc tttatcagtc tgattaaagg agtacgtagc cgtgataaaa
    24601 cgtaatttgc cactgatgat caccatcggc gtctttgtgc tgggctattt ctactgcctg
    24661 acgcagtttc ccggttttgc ttccacaaga gtgatctgca atatcctgac cgataacgct
    24721 tttctgggga ttattgccgt tggtatgacc tttgtgatcc tctctggcgg tatcgatctc
    24781 tccgtcggtt cggtgatcgc ttttaccggc gtgtttctgg caaaagtgat tggcgacttc
    24841 ggcctctcgc cgttgctggc atttccgctg gtgctggtaa tgggctgtgc ctttggcgca
    24901 tttatggggc ttctgatcga cgccctgaag atcccggcat ttatcattac gcttgcgggg
    24961 atgttctttt tgcgcggcgt cagctatctc gtttcggaag agtcgattcc gataaaccat
    25021 cccatttatg acacgctctc aagccttgcg tggaaaatcc ctggcggcgg tcgcttaagt
    25081 gcgatggggc tgctgatgct ggcggtggtg gttatcggca tattcctcgc gcatcgtacc
    25141 cgttttggta atcaggtata cgccattggc ggcaacgcaa cgtcggcaaa cctgatgggg
    25201 atttctaccc gcagcaccac tattcgcatt tatatgctct ccaccggact ggcaacgctg
    25261 gcggggattg tcttctcgat ttatacccag gccggatatg cgctggcggg cgtaggtgtg
    25321 gaactggacg ctatcgcctc agtggtgatt ggcggtacgc ttttgagcgg tggcgttgga
    25381 acggtattag ggacgctttt tggcgtggcg attcagggac tgattcagac ttacataaac
    25441 tttgatggca cgctgagttc ctggtggacg aaaatcgcca tcggtatttt gttgtttatt
    25501 tttatagcat tacagcgtgg attaacggtg ctgtgggaga atcgtcagag ttcgccagtg
    25561 acaagagtca acattgcgca gcaataaaaa cgcctctccg tgtggagagg cgcaggagat
    25621 tacgcgtccg ggaactcacg gataaagcgt tcgacatctt caaccatatg gtcgttgccg
    25681 acaaagaacg aacggcgctg gtgcagggtt tccgggataa tatccagaat acgctctttg
    25741 ccatcgctcg ctttaccgcc cgcttgttcc gccaggaatg ccatcgggtt gcactcatac
    25801 agtaaacgca gtttaccgtc cgggtggctg gcggtgcttg ggtagagata aataccgcct
    25861 ttcagcaggt tacggtggaa atccgcaacc agtgaaccga tataacgtga ggtatacggg
    25921 cggttggtgg atttatcttc ttcctggcag aatttaatgt acttcttcac cccgttcgga
    25981 aacttaatgt agtttccttc gttgatggag taggttttgc ctttctccgg gaagcgcatc
    26041 cgttcctggc acaggcagaa aacgcccagc gaaggatcgt aagtaaaggc gtgaacaccg
    26101 catccggtgg tgtaaaccag catagtagag gagccgtata ccacgtaacc tgccgcaacc
    26161 tgtttgttac caggctggag gaaatcttct tccgttaccg gcgtgccaac aggcgtaacg
    26221 cggcggtaga tggagaaaat ggtaccgaca gagacgttaa catcgatgtt ggacgagcca
    26281 tccagggggt ccatcagcac cacgtatttt gcgtgttcac agccttcaaa gacgacaatc
    26341 tcatcttctt cttcagaggc aatgcccgca acgatatcgc gtgctttcag tgcggctttc
    26401 agtttttcat tagcgaacaa gtcgagtttc tgctgaacct cgccctgcac gttctcagca
    26461 ccgctggcac ccaggatatc aaccagtcct gctttgttga tatcgcgatg gataatcttg
    26521 gcgcccagtt ttattgccga cagcaaagca gtgagctcac cggtagcatg agaaaactcg
    26581 tgctgctttt cgacaataaa ttcacctaac gttttcataa aactttccct gcaatgttta
    26641 tggagtaaag cgaccgcaac aatcttaaca aataatctca atgttgcgct caggtgaatc
    26701 gcgccagcaa attgcggatt atcctgaaat gcgtttctca cttgcccgac atatgcgtaa
    26761 aatgagcggc agattaaaaa aggatagtga cgtatgcgca ttcatatttt aggaatttgt
    26821 ggcacgttta tgggcggtct ggcgatgctg gcgcgccagt taggccatga agtaacgggt
    26881 tcggacgcca atgtgtatcc gccgatgagc accttacttg agaagcaagg cattgaactg
    26941 attcagggtt acgatgccag ccagctcgat ccgcagccgg atctggtgat tattggcaac
    27001 gccatgaccc gtggaaatcc gtgtgtggaa gcggtactgg aaaaaaacat cccttatatg
    27061 tcaggtccac agtggctgca cgattttgtg ctgcgcgacc gctgggtgct ggccgttgcc
    27121 ggtacacacg gcaaaaccac caccgcggga atggcgacct ggattctgga acagtgtggt
    27181 tacaaaccgg gctttgtaat cggcggtgtg ccggggaact ttgaggtttc ggcgcgtctg
    27241 ggcgaaagcg acttctttgt tatcgaagcg gatgagtatg actgcgcctt cttcgacaaa
    27301 cgctctaaat ttgttcatta ctgcccgcgt acgctgatcc tcaacaacct tgagttcgat
    27361 cacgccgata tctttgacga cctgaaagcg atccagaaac agttccacca tctggtgcgt
    27421 atcgtaccgg ggcatggccg tattatctgg ccggaaaatg acatcaacct gaaacagacc
    27481 atggcgatgg gctgctggag cgagcaggag ctggtgggtg agcagggtca ctggcaggcg
    27541 aaaaagctga ccaccgatgc ttccgaatgg gaagtcttgc tggatggcga aaaagtgggc
    27601 gaagtgaaat ggtcgctggt aggcgaacat aatatgcaca atggcctgat ggcgattgcg
    27661 gcggctcgcc atgttggtgt agcgccggca gatgccgcta atgcgctggg ttcgtttatt
    27721 aacgctcgtc gccgtctgga actgcgtggt gaagcgaatg gcgtcacggt atatgatgat
    27781 ttcgcccatc acccaacggc gattctggca acgctggcgg cgctgcgtgg caaagttggc
    27841 ggtacggcgc gcattattgc tgtgctggaa ccgcgctcga ataccatgaa aatggggatc
    27901 tgcaaagacg atctggcacc ttcattaggc cgtgccgatg aagtcttcct gctgcaaccg
    27961 acgcatattc cgtggcaggt ggcagaagtg gcagaagcct gcgttcagcc tgcacactgg
    28021 agtggcgatg tggatacgct ggcagatatg gtggtgaaaa ccgctcagcc tggcgaccat
    28081 attctggtga tgagcaacgg cggttttggt ggcatccatc agaaactgct ggatggtctg
    28141 gcgaagaaag cggaagccgc gcagtaattc ggccttagcc tgatatagtg tgcgtaggcc
    28201 ggataaggcg ttcacgccgc atccggcatt tgagcatagt gcctgatgcg acgcttgatg
    28261 cgtcttatca ggcctacagg tgcaccgcat ccggcaaacc atactcacat caacaacgaa
    28321 aattaccctt cattctccgc cagttcgcgc agatactgga aaatctggcg tgcggatttc
    28381 ggcggcttat tcccttcttt ctctttcttc gcgttgcgga tcagagtacg cagctgctga
    28441 cgatcggcat cgggccacag atttaatacc tcagcgatgg cgtcatcacc ttgatcgatc
    28501 agacgatcgc gcaggttttc cagtttatgg aacagcacca cctgctggtt gtgacggttt
    28561 ttcagcttat ccagcgcctg acggatcggt tctacatcgc gctggcgcag catcttaccg
    28621 atgagctgca actggcggcg gcgaccttcc atcttaatac gctgggcgag ctcaatagcc
    28681 gcgcgcagat ccgcatctaa cgggatctta tccagcgcgt ttttccccag atcaacaatt
    28741 tccgcgccaa ggcgttttag ctcctcggca tcacgtttaa tttcactttt actgacccag
    28801 ataatttcat cgtcttcgtc ttcgatgtca tcaccgggaa cgtcgtcgag ccagtcttcg
    28861 ggctgcttag tcatctcagg ctccttaaaa aaagaggcta atgttaccag ttaagatgcg
    28921 cactgaaaaa cggttctctg ttagacttca gagaaactct ctacattatg gcacttgcaa
    28981 tgaaagtaat ctctcaagtt gaagcgcagc gcaagattct ggaagaagca gtttccactg
    29041 cgctggagtt ggcctcaggc aaatcggacg gtgcggaagt tgccgtcagc aagaccaccg
    29101 gcattagcgt aagcacgcgt tatggtgaag tggagaatgt tgaattcaat agcgatggcg
    29161 cgctggggat cactgtttat caccagaacc gcaaaggtag cgcatcatcc accgatttaa
    29221 gcccgcaggc cattgcccgt actgtacagg cggcgctgga tattgcccgt tatacctcgc
    29281 cagatccctg tgccggcgtg gcagacaaag agctgctggc ctttgacgca ccagatctcg
    29341 acttgttcca ccctgcggaa gtttccccgg atgaagccat tgaactggcg gcccgcgcag
    29401 aacaggcggc attgcaggcg gacaaacgca ttaccaatac cgaaggtggc agctttaaca
    29461 gccactacgg tgtcaaagtt tttggcaaca gccacggcat gttgcagggt tactgctcaa
    29521 cgcgtcattc gctctccagc tgtgtaattg ccgaagaaaa tggcgatatg gagcgtgatt
    29581 acgcctacac cattggccgc gcgatgagcg atctgcaaac gccagagtgg gtcggggccg
    29641 actgtgctcg ccgtacttta tcgcgcctgt caccgcgtaa actctccacc atgaaagcgc
    29701 cagtcatttt tgccaatgaa gtggcaaccg ggctttttgg ccatctggtg ggggcgatag
    29761 cgggtggctc ggtttaccgt aaatctacct ttctgctgga ttcgctgggt aaacaaattc
    29821 tgccggactg gctgaccatt gaagagcatc cgcatctgct gaaagggctg gcgtcgacgc
    29881 cattcgacag cgaaggtgtg cgcaccgagc gtcgcgatat tattaaagat ggcatcctga
    29941 ctcagtggct gctgaccagc tactcggcgc ggaaactggg gctgaaaagc accggacatg
    30001 cgggcggtat tcacaactgg cggattgccg gacaagggct aagcttcgaa caaatgctca
    30061 aagagatggg caccgggctg gtggtgacgg agctgatggg ccagggcgtg agtgccatta
    30121 ccggtgatta ttcccgtggt gcagcgggct tctgggtaga gaatggcgaa attcagtatc
    30181 ccgttagtga aatcaccatc gcaggtaatt taaaagatat gtggcgcaat attgtcaccg
    30241 tcggtaacga tattgaaaca cgcagtaata tacagtgtgg ttctgtgctg ttgccggaga
    30301 tgaaaatcgc cgggcagtaa aaaactggcg cgacctgccg cgctataaac taaaaaatta
    30361 cacaaaatca ttcgcactgc atcgagacgg aaagtgaatg agggccgggg agcgtactcg
    30421 cagtacgtga cccgggcgaa tgagcgtagc cgtcgatgag gcagtgtgaa ggatgaagtg
    30481 taaataaaaa aggaagtgag caatgcgtaa aagcctgtta gctattcttg cagtctcctc
    30541 gttggtattc agttctgcgt cgtttgccgc tgatcttgaa gacaatatgg aaaccctcaa
    30601 cgacaattta aaagtgatcg aaaaagcgga taacgcggcg caagtcaaag acgcgttaac
    30661 gaagatgcgc gccgcagcgc tggatgcgca aaaagcaacg ccgccgaagc tcgaagataa
    30721 atcaccggac agcccggaaa tgaaagattt ccgccacggt ttcgacattc tggtcggtca
    30781 gattgacgac gcgctgaagc tggcaaatga aggtaaagta aaagaagcgc aggctgctgc
    30841 agagcaactg aaaacgaccc gcaacgccta tcaccagaag tatcgttaat tcctcatttc
    30901 cctgttgcct gcactcaggt aacagggaac ttatcatcgc aaatgatgca ccacctgatt
    30961 gctgctgccg cgccagatca gggaagggtc ttttaaatcc tgcacaaatt tgccgtcgac
    31021 cagcacgtta atcaaatcaa caacctgcat ttgcgcagcg ttgagttcgt cgagtttata
    31081 gcctgtccac acccagatgt ctttacccgg acactcggcg cggatgcgtt ttaccagttt
    31141 cagaatatcc ggcacgtttt gcggatgcag cggatcgccg ccggagaggg aaataccctg
    31201 gcgtttgata cgagtgtcat tcagatcgtt aataatctgg tcttccattg ctttagtaaa
    31261 tgtctgaccg gaatttaccc gccatgtgct tttgttatag caaccggggc attcatgaac
    31321 acacccggag acaaacaggg tgcagcgagt gccggggccg ttgacgatgt cgacaggata
    31381 gtactgatga taattcattg tttcgcctgg cttatcagtc cgcgtatagg atgcatccgg
    31441 taatcaatgc ctgatgcgac gctgtcgcgt cttatcaggc ctacagccgt tgccaaatgt
    31501 aggccggata aggcgtttac gccgcatccg gcatttgctt agcgcagagt gaagattaac
    31561 ctatctgccc attccccaaa tgtttaacgc ggcgcttaac ttcttcctgc ttaccagcgt
    31621 taaatggacg tgcatccggg ctacctaaat atccgcacac gcggcgagtc accgacacac
    31681 gggaggcgtc atggttacca catttcgggc aagtgaagcc tttgctggtg cactcgaact
    31741 caccggtaaa gccacactcg tagcactcat caatcggtgt attggtgccg taatacggta
    31801 catgctgata gctgtaatcc cagacatctt ccagcgcctt caggttgtgc tgaatgtttg
    31861 gatactcgcc gtagcaaatg aaaccaccgt tcgccagcgg cgggtaaggc gcttcaaagt
    31921 cgatcttgtc gtacgggttc accttcttct ccacatcgag gtggaaactg ttggtgtagt
    31981 aacctttatc ggtcacgccc ggcaccacgc caaactcagc agtatcgaga cggcagaagc
    32041 ggtcgcacag gttttcactc ggcgtgctgt agagactgaa accataaccc gtttcttctt
    32101 tccactgatc cactgcctgg cgcagacgtt caacaatggc gatacctttc gcgcgaagct
    32161 gctcgttgtc gtaaacatgc tcgccgccga acagcgcgtt aatggtttcg tggatgccga
    32221 tgtaacccag cgaaatagac gcacgaccgt ttttgaagat ttcagaaaca tcatcgtcag
    32281 cattcagacg tacgccacaa gcaccttcca tatagaggat cggggcgacg cgcgctttca
    32341 cgccttcgag acgagcgata cgggtcatca gtgccttacg tgccagcacc agacgttcat
    32401 ccagcagctt ccagaaggtg gcttcatcgc cttttgcttc cagagcaata cgcggcaggt
    32461 tcaggctgat cacgccgagg ttgttacgac catcgtggat ctgctcgccg ttttcatttt
    32521 cccacacgcc gaggaagctg cggcagccca tcggggtttt aaacgaaccg gtgactttca
    32581 ctacctgatc gtagttcagg atatccggat acatgcgctt gcttgcgcac tccagcgcca
    32641 gctgtttgat gtcgtagttc ggatcgcctt ttttatggtt caggccatcg cgaatcgcaa
    32701 acaccagttt cgggaacacc gcagttttac ggtttttacc gaggcctgcg atacggttac
    32761 gcaggatcga ttcctgaatc aggcgcgatt cccagctggt gcccagacca aaaccaaagg
    32821 ttacaaacgg cgtctgaccg ttggcggtgt gcagggtgtt tacttcgtac tccagcgact
    32881 ggaaggcatc gtagcactct ttgatggtac gagagttagc gtagccttcg gcgtccggga
    32941 tgttccactc ttctgcggtc ttgcgatgtt tgttgtagct ggcggtaaca aacggcgcca
    33001 gcacttcatc gatacggtta atggtggtgc cgccataaat atggctggcg acctgagcaa
    33061 taatctgcgc agttaccgcg gttgccgtag agatcgactt cggcggttca atctcggcgt
    33121 tccccatttt aaagccctgg gtcagcatgc ctttcaggtc gatcaacatg cagttgaaca
    33181 tcgggaagaa cggcgagtaa tcgagatcgt gatagtgaat atcgccacgc tcatgtgcct
    33241 gcaccacgtc acgcggcagc aggtgctggc gtgcatagtg tttagccacg atcccggcca
    33301 gcaggtcgcg ctgggttgga atcaccttgc tgtctttgtt ggcgttttcg ttgagtaacg
    33361 aggcgttggt ctgctcgacc agaccacgga tctcctggtt caggcgaccg cgtttttcac
    33421 gttcaatgtc gcgatcgtga cggtactcga tgtaagcacg agccagttgt ttgtatggac
    33481 ccgacatcag ctggttttca actgcggtct ggatctcgtt gatatccacc tggttgcggc
    33541 cctgcatctg ctcgctgaca accgcggcaa cagtggcgca ataatcggca tcatcgactt
    33601 ccgctgcttt agctgcacgc agaatcgctt ctttgatgcg ctctgattta aacggcactt
    33661 tgcagccgtc tcgtttcatc acatgcggtg tcatgatcgc tccatatttt taagaacagg
    33721 ttatccacag aaattgggaa aggcgttttc cggttttttc gtttcgtttt ccggtgcttt
    33781 ccgcaatcgc catccgctta tccacaacgc cgcacctttt tcgtggggag ctgtagtagc
    33841 aattatagtc gattaataca acatattggg ttgggacgca ttttaaagtc tatatatagt
    33901 gctttgcatc aaggatgttt gaactttttt tgatgtagct caaagtaaaa agcagagcgt
    33961 acggatgacg ggcgctacag cgttatgtaa attttttaat gaatttgctg gttgaaaaat
    34021 caacaaaaac aacatgctga cagacaaaac cccggaatga ccggcattac cggggcttag
    34081 ggaagattta cttctgtaac caccagacag cctcaaaagg ccgtaaattc atggcacagg
    34141 gttgtggtga ggcttcttcg tagttatgca tcacaagctg ccagttgccg cgcatttgcc
    34201 ctggctgcca gggttggatc tcacggctaa ggttggcaat gaccagcaag gtttgcccct
    34261 tccattcacg gcgatagcac cacaatacag ggctgtttgg cagcagatcc tggtaattgc
    34321 cccatgtcag gatggcttct tgcttacgca gtgcgattaa cttttggtag gtgtaaaaca
    34381 ccgaggactc atcggccagc gcggcttcta cgttgatttg ttgatagtta tcgcccaggc
    34441 caatccacgg ttcgccagcc gtaaacccgg cattatcgcc gttgctccat tgcatgggcg
    34501 tgcggctgtt gtcacgggat ttactggcga ggattgccaa taactcgtcg gcatcacgac
    34561 catcgttgcg cagctcggca aacatattga ggctctccac gtcgcgatag tcagtaatgc
    34621 gcgtgaaatg cgggttggtc atgccaatct cttcgccctg gtagatatac ggcgttccct
    34681 gcatgccatg cagcaccatc gccagcattt ttgccgcagg cacgcggtat tcaccttcat
    34741 caccaaagcg agaaacaatg cgcggctgat cgtggttaca ccagaacaag gcattccatg
    34801 ccacgttgtg cattccttgt tgccagtggc ggaacaatgt tttcaacgcc acaaagtcag
    34861 gtttagccag cgtccatttt tcaccaccgg gataatcgac cttcaggtga tgaaaattaa
    34921 aggtcatcga caattcactg cctgtcagtg ccgcgtatcg ctggcaatgc tcaaggctgg
    34981 tggaggacat ttcacctacg gtcattaacc cgcgtggcgt aaacacatcg cggttcatct
    35041 cgtgcaaaaa ctcgtgtgct cgtggcccgt cggtgtagaa gcgacgcccg tcgccatcca
    35101 ggtcttcagg gaaacgcggg tctttggaga tcagattcac cacatccagg cgcaacccgt
    35161 cgaccccacg atcggcccag aattcacaga cttttttcag ctctgcgcgt accgctggat
    35221 tctcccagtt gagatccgcc tgttctggtg caaagagatg caaatagtac tgttcgcttt
    35281 ccgcatgcca gcgccacgca ctaccgccaa attttgaacg ccagttgttc ggtggcgttt
    35341 ctggttcgcc atcgcgccag atataaaact ggcggtaagg gctttctttg ttcagcgcct
    35401 cgcgaaacca ggcatgttgg gtagaggtat ggttaaacac catatcgaga atgatacgaa
    35461 tcccgcgcga ttttgcctgc gtcaccagtt cgtcaaaatc gtccagcgtg ccgtagatgg
    35521 gatcaatcgc cgtatagttc gctacgtcgt aaccgttatc gacctgggga gagatataaa
    35581 agggggttag ccagatggca tcaacgccca gtttatgcag atagtccagg cgttggataa
    35641 cgccacgtaa atcgccggtg ccgctacccg tggtgtcctg aaaactcttt ggataaatct
    35701 ggtagataac gccgttttgc caccagtggg gaagattagt cattacgtta ttcctgcaat
    35761 tgcgccccga agaaaattaa acaatatcca gcgtgcccag gcggtatttc cgctgataga
    35821 taaacgaggt gagtacaatc gggatgatga tggcgataac cattgccagc gcaaatacct
    35881 gccagtagct cggttgaatc gagagaattc ccggcaggcc gcctacgccg ataccattcg
    35941 ccataacgcc gttcaggccg catagtaatc ctgccagacc agaaccaatc atcgcgcaca
    36001 gcatcgggaa gcgatatttc aggttgatgc cgtacattgc aggctcagtg accccaagcc
    36061 aggcggagat agcggcaggc acggagatct cgcgttcatt gtgcttgcgg ctggaaatga
    36121 taatgcctat cacggcggag ccctgagcga tattcgacag cgcaatcagc ggccacactg
    36181 gcgtgccacc catgctttga atcatctgca aatcaatagc aagggtggtc tggtgtacac
    36241 cggtgatcac cagcggggcg tacaggaagc caaacaatgc tgcgccaatc ggagcaaagc
    36301 tgccggtcat caggtgacgt accgcaaagg caacgccatc gccaatcatg cgaccaaacg
    36361 gaccaatcag cgcatgggcg aggaacaccg cgaggatcag cgaacagacg ggcaccacca
    36421 ccagatagag gtaatccggc acgatgcgtt taaggcgagt ttcaataacg cccagcgcca
    36481 gcccggctaa cagtgccggg atcacctgcg cctgatagcc tactttggcg atgctgaaca
    36541 tgccaaagtc ccacacttcc ggcagctgct gcccgagcag ataagcgttc atcagctggg
    36601 gagaaaccag tgtcacgcca agcacgatac caaggatcgg cgtgccgccc atttttttca
    36661 ccgctgacca gcaaatcccg accggcaggt agaagaagat cgcttcaccg atcaaccaca
    36721 gaaaatcgta gatcgtttgc agggaagggt acatttgcgc cagcgtttga ccgttgctca
    36781 tgggcaaatc gccgatcaca ttgcgaaaac cgaggatcaa accgccgcta atcaacgcgg
    36841 gcagcaacgg gaagaagatc tccgcgaaat gagagatcaa ctgctcatgc catttcatat
    36901 tctgccgggc ggcttttttt acctgctctt tatcaacctg cgcctgtccg gttgacgcta
    36961 ttagtgcttg atagtaatca cccacgttgg tgccaatcac cacctgaaat tgcccggcat
    37021 tggtgaaaca gcctttcacc atggggagtt gctcaatttc tttcggtctg gcattggccg
    37081 gttggttgag gacaaagcgc aggcgagtaa tacagtggct caccgtcgca atattgccgc
    37141 gcccgccgac cagttcaatc aaccgatcga tatccgtttg gtttattttg ctcatcataa
    37201 agccccatgg cagatgacat ttttggttgg ctgcagaata tattgcgcgg aaaaatttaa
    37261 aaacgggaac gttcccgaag cgcagcgaag atcacaattt atcgttcagg aaacgatcag
    37321 gacagggtgg cggggatgat gatttgttgc ggttcgctgc gcccggttac ctgtgcgatc
    37381 aattggcagg ccgcctggcg tccagcttcg gcgtaaccag gatctacggt gacgatctcc
    37441 ggatgaagga atttcattaa tggcgtatta ccgacgctcg ccagttgcaa ggtgtcgatg
    37501 cgttgctctt gcaggtattt acttgcgcca agtgccagcg tgtcggttgc gcacagtaag
    37561 gcggtagttt caggcgtaat cacttttgct acgttttcat agccttgctt catagcaagc
    37621 cctggcagag cggcaacggg atgcagttta tgcgctttgc agaacgccag gtaggcttcg
    37681 tgacgtcgct tcccggtcgt cacgtcacta tgcggcacgc cgagataact gatattgcga
    37741 tgcccctggt catagagccg ttgcatcagg atcttgattg ccccttcgtc gtcataacag
    37801 accgaagcaa agccttttgc gtcacgcgcc agcagaacca gcgatgactg ccagtgggct
    37861 aacatttctt ctgttatgcc agtaaaaccg aacaacacta cgccgtcgat attacgccgt
    37921 ttcagcaccc ccaaatgttc ggcaactaat tgcggggaaa actgactttc catcatgatt
    37981 gggtcgtaac cttgttcata gaacgctggc agcatggttt gaacagcgag attttctgac
    38041 aacgaatcca ggcgggtaac aatgatggcg accactttat cgctttgccc acgcatagcg
    38101 cgcgcagagc gggaagggga aaatccatgc tgattcatca ctgcttcaac acgctcgcgg
    38161 gtgcgctggc tcacgccgct ttcgttattc agcacccggg aaaccgtaga tttccccacg
    38221 ccgcttaagc gcgcgatgtc tttgatggtc agccgatttt gcatcctgtt gtcctgtaac
    38281 gtgttgttta attatttgag cctaacttta cccgtgcatt cagcaatggg taaagtctgg
    38341 tttatcgttg gtttagttgc cagcaggtat tatatcgcca tagatgctac gaatattatt
    38401 ggattctcct tattatttgc ggcgcttttt tcacttaccg gaggttatat ggaacctgat
    38461 cccacgcctc tccctcgacg gagattaaaa cttttccggt aagcccgtct tttcacggcg
    38521 ttaccggatg cgtaaggccg tgacgtttta acgtccctgc tcagctttat taccttcagg
    38581 taaggcttcg ccacgcctga agacatttct gtactgtttc agacagtgcg gagggactcc
    38641 ttatgtttaa agaaattttt acccggctca ttcgccattt accttcccgt ctggttcatc
    38701 gcgatccatt gcctggcgcg cagcagacag tgaatacggc ggtcccgccg tccttaagtg
    38761 cgcattgcct gaaaatggcg gtgatgcccg aagaagaatt atggaaaacg ttcgacaccc
    38821 atccggaagg gttaaatcag gcggaagtgg aatctgcccg cgaacaacat ggtgaaaata
    38881 aattacccgc acaacaaccg tcgccgtggt gggtacattt atgggtctgc tatcgcaacc
    38941 cctttaatat tttactcacc attctcggcg ctatttctta cgccacggaa gatttatttg
    39001 ccgcgggcgt tattgcgcta atggtcgcta tttcaacgtt actgaacttt attcaggaag
    39061 cacgttccac taaagcggca gatgccctga aagcgatggt cagcaatacc gcgacggtgc
    39121 tgcgcgtaat taacgacaaa ggcgaaaatg gctggctgga gatcccgatc gaccagctgg
    39181 tgcccggcga tattataaaa ctggcggcgg gagatatgat cccggcagat ttacgtatct
    39241 tgcaggcgcg ggatctgttc gtcgctcagg cgtcgttaac cggtgagtct ctgcccgtgg
    39301 aaaaagccgc taccactcgc cagccggagc acagcaatcc gctggagtgc gacacgctgt
    39361 gttttatggg caccaccgtg gtgagcggca cggcacaagc aatggtgatt gctacaggtg
    39421 ccaatacctg gtttggtcaa ctggcggggc gtgttagtga gcaggaaagc gagccgaatg
    39481 cctttcagca ggggatcagc cgcgtcagta tgctgctgat tcgctttatg ctggtgatgg
    39541 ctccggtggt gttgcttatc aacggttaca ccaaaggcga ctggtgggaa gcggcactgt
    39601 ttgcgctttc ggtcgcggta ggcttaacgc cggaaatgtt gccgatgatt gttacctcga
    39661 cgctggcgcg cggcgcagta aagctgtcga aacagaaagt gatcgtcaaa catctggatg
    39721 ctattcagaa ctttggcgca atggatattc tgtgcactga taaaactggc accctgacac
    39781 aggacaaaat tgtgctggag aatcataccg atatctccgg taaaaccagc gaacgcgtgc
    39841 tgcatagcgc gtggttgaac agtcattatc agaccggact taaaaacctg ctcgatacag
    39901 cggtgctcga aggtacggat gaagagtcag cgcgctcgct ggccagtcgt tggcaaaaaa
    39961 ttgatgagat tccgtttgat ttcgagcgtc gccggatgtc ggtggtagtg gcagaaaata
    40021 ccgagcacca tcagctggtt tgcaaaggtg cattgcagga aatcctcaat gtgtgttcgc
    40081 aggtgcgtca caatggcgag attgtgccgc tcgatgacat catgctgcgt aagattaagc
    40141 gggttactga tacgcttaat cgtcaggggc tgcgcgtggt tgcggtggcg acgaaatacc
    40201 tgccagcgcg tgaaggagat taccagcggg cggatgaatc cgacctgatc ctcgaaggat
    40261 atattgcttt ccttgatccg ccaaaagaga caaccgctcc ggcactgaag gcattaaaag
    40321 cgagtggaat caccgtaaaa atccttactg gcgacagtga gttagttgca gcgaaagtgt
    40381 gccatgaagt ggggctggac gcgggagagg tggtcattgg tagtgatatt gaaacgctat
    40441 ctgacgacga actggcaaat ctcgcacagc gcaccacgct gtttgcccgc ctgacgccga
    40501 tgcataaaga acgcattgtg accttactga agcgcgaagg gcatgtggtt ggctttatgg
    40561 gcgatggtat taatgatgcg cccgccttac gcgctgcgga tatcggcatt tctgtggacg
    40621 gcgcggtaga tattgcccgt gaagcggctg atatcatcct gctggaaaaa agcctgatgg
    40681 tgctggaaga gggggttatt gaaggacgtc gcactttcgc caacatgctg aaatacatca
    40741 aaatgacggc gagctccaac ttcggtaatg tgttcagcgt gctggtggcg agtgctttct
    40801 tgcccttcct gccgatgttg ccgttacact tgcttattca gaacctgctg tatgatgtgt
    40861 cacaggtggc gatcccgttt gataacgtcg acgacgagca aattcaaaag ccgcagcgtt
    40921 ggaatccggc ggatctgggg cgctttatga tcttcttcgg accgatcagc tcgatcttcg
    40981 atattctgac gttttgcctg atgtggtggg tattccatgc caacacgccg gaaacgcaaa
    41041 cgctgttcca gtcgggatgg tttgtggtgg gcttactgtc gcaaacgctg attgtgcata
    41101 tgatccgcac ccgccgtgtg ccgtttattc agagctgtgc atcgtggccg ttaatgatca
    41161 tgaccgtgat cgtgatgatt gtcgggatcg cattgccgtt ttcaccgctg gccagttatc
    41221 tgcagctgca ggcgctgccg ttaagctatt tcccgtggct ggttgcgatt ctggcagggt
    41281 atatgacatt aactcagttg gtgaaaggat tttatagccg tcgttacggc tggcagtaaa
    41341 gaataaactg ggcacgatag ccccgattca gattgcagac aaagtaagct ttgttcatgc
    41401 cggatgcggc gtaaatgcct tatccggcca acaaattcaa taaattgcag ga
//
LOCUS       DAZGAN020000033        40990 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGAN020000033 DAZGAN020000000
VERSION     DAZGAN020000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.033"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(97..996)
                     /gene="lysO"
                     /locus_tag="ACHKYO_RS09810"
                     /old_locus_tag="pgaptmp_001962"
     CDS             complement(97..996)
                     /gene="lysO"
                     /locus_tag="ACHKYO_RS09810"
                     /old_locus_tag="pgaptmp_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HGU4428819.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHTTEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(1491..2186)
                     /gene="aqpZ"
                     /locus_tag="ACHKYO_RS09815"
                     /old_locus_tag="pgaptmp_001963"
     CDS             complement(1491..2186)
                     /gene="aqpZ"
                     /locus_tag="ACHKYO_RS09815"
                     /old_locus_tag="pgaptmp_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HGU4428820.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAELAAGFSELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            2612..4270
                     /gene="ybjD"
                     /locus_tag="ACHKYO_RS09820"
                     /old_locus_tag="pgaptmp_001964"
     CDS             2612..4270
                     /gene="ybjD"
                     /locus_tag="ACHKYO_RS09820"
                     /old_locus_tag="pgaptmp_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HGU4428821.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(4267..5223)
                     /gene="ybjX"
                     /locus_tag="ACHKYO_RS09825"
                     /old_locus_tag="pgaptmp_001965"
     CDS             complement(4267..5223)
                     /gene="ybjX"
                     /locus_tag="ACHKYO_RS09825"
                     /old_locus_tag="pgaptmp_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HGU4428822.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            5374..6489
                     /gene="macA"
                     /locus_tag="ACHKYO_RS09830"
                     /old_locus_tag="pgaptmp_001966"
     CDS             5374..6489
                     /gene="macA"
                     /locus_tag="ACHKYO_RS09830"
                     /old_locus_tag="pgaptmp_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HGU4428823.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            6486..8432
                     /gene="macB"
                     /locus_tag="ACHKYO_RS09835"
                     /old_locus_tag="pgaptmp_001967"
     CDS             6486..8432
                     /gene="macB"
                     /locus_tag="ACHKYO_RS09835"
                     /old_locus_tag="pgaptmp_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HGU4428824.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(8505..8729)
                     /gene="cspD"
                     /locus_tag="ACHKYO_RS09840"
                     /old_locus_tag="pgaptmp_001968"
     CDS             complement(8505..8729)
                     /gene="cspD"
                     /locus_tag="ACHKYO_RS09840"
                     /old_locus_tag="pgaptmp_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HGU4428825.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            8879..8953
                     /gene="yljB"
                     /locus_tag="ACHKYO_RS09845"
                     /old_locus_tag="pgaptmp_001969"
     CDS             8879..8953
                     /gene="yljB"
                     /locus_tag="ACHKYO_RS09845"
                     /old_locus_tag="pgaptmp_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HGU4428826.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            9052..9372
                     /gene="clpS"
                     /locus_tag="ACHKYO_RS09850"
                     /old_locus_tag="pgaptmp_001970"
     CDS             9052..9372
                     /gene="clpS"
                     /locus_tag="ACHKYO_RS09850"
                     /old_locus_tag="pgaptmp_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HGU4428827.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            9403..11679
                     /gene="clpA"
                     /locus_tag="ACHKYO_RS09855"
                     /old_locus_tag="pgaptmp_001971"
     CDS             9403..11679
                     /gene="clpA"
                     /locus_tag="ACHKYO_RS09855"
                     /old_locus_tag="pgaptmp_001971"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HGU4428828.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(12023..12110)
                     /locus_tag="ACHKYO_RS09860"
                     /old_locus_tag="pgaptmp_001972"
     tRNA            complement(12023..12110)
                     /locus_tag="ACHKYO_RS09860"
                     /old_locus_tag="pgaptmp_001972"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12074..12076),aa:Ser,seq:gga)
     gene            complement(12364..12582)
                     /gene="infA"
                     /locus_tag="ACHKYO_RS09865"
                     /old_locus_tag="pgaptmp_001973"
     CDS             complement(12364..12582)
                     /gene="infA"
                     /locus_tag="ACHKYO_RS09865"
                     /old_locus_tag="pgaptmp_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGU4428829.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(12867..13571)
                     /gene="aat"
                     /locus_tag="ACHKYO_RS09870"
                     /old_locus_tag="pgaptmp_001974"
     CDS             complement(12867..13571)
                     /gene="aat"
                     /locus_tag="ACHKYO_RS09870"
                     /old_locus_tag="pgaptmp_001974"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HGU4428830.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(13613..15334)
                     /gene="cydC"
                     /locus_tag="ACHKYO_RS09875"
                     /old_locus_tag="pgaptmp_001975"
     CDS             complement(13613..15334)
                     /gene="cydC"
                     /locus_tag="ACHKYO_RS09875"
                     /old_locus_tag="pgaptmp_001975"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HGU4428831.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDPQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMSEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(15335..17101)
                     /gene="cydD"
                     /locus_tag="ACHKYO_RS09880"
                     /old_locus_tag="pgaptmp_001976"
     CDS             complement(15335..17101)
                     /gene="cydD"
                     /locus_tag="ACHKYO_RS09880"
                     /old_locus_tag="pgaptmp_001976"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HGU4428832.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(17224..18189)
                     /gene="trxB"
                     /locus_tag="ACHKYO_RS09885"
                     /old_locus_tag="pgaptmp_001977"
     CDS             complement(17224..18189)
                     /gene="trxB"
                     /locus_tag="ACHKYO_RS09885"
                     /old_locus_tag="pgaptmp_001977"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGU4428833.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            18734..19228
                     /gene="lrp"
                     /locus_tag="ACHKYO_RS09890"
                     /old_locus_tag="pgaptmp_001978"
     CDS             18734..19228
                     /gene="lrp"
                     /locus_tag="ACHKYO_RS09890"
                     /old_locus_tag="pgaptmp_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HGU4428834.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            19363..23508
                     /gene="ftsK"
                     /locus_tag="ACHKYO_RS09895"
                     /old_locus_tag="pgaptmp_001979"
     CDS             19363..23508
                     /gene="ftsK"
                     /locus_tag="ACHKYO_RS09895"
                     /old_locus_tag="pgaptmp_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGU4428835.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQLYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQY
                     QQPQQPVAPQQQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALE
                     QMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTV
                     AVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVV
                     ADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIP
                     HLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPD
                     PYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHL
                     VLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPN
                     STLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPL
                     FDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPP
                     PFD"
     gene            23663..24274
                     /gene="lolA"
                     /locus_tag="ACHKYO_RS09900"
                     /old_locus_tag="pgaptmp_001980"
     CDS             23663..24274
                     /gene="lolA"
                     /locus_tag="ACHKYO_RS09900"
                     /old_locus_tag="pgaptmp_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HGU4428836.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            24285..25628
                     /gene="rarA"
                     /locus_tag="ACHKYO_RS09905"
                     /old_locus_tag="pgaptmp_001981"
                     /gene_synonym="mgsA"
     CDS             24285..25628
                     /gene="rarA"
                     /locus_tag="ACHKYO_RS09905"
                     /old_locus_tag="pgaptmp_001981"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HGU4428837.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            25719..27011
                     /gene="serS"
                     /locus_tag="ACHKYO_RS09910"
                     /old_locus_tag="pgaptmp_001982"
     CDS             25719..27011
                     /gene="serS"
                     /locus_tag="ACHKYO_RS09910"
                     /old_locus_tag="pgaptmp_001982"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGU4428838.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            27250..29694
                     /gene="dmsA"
                     /locus_tag="ACHKYO_RS09915"
                     /old_locus_tag="pgaptmp_001983"
     CDS             27250..29694
                     /gene="dmsA"
                     /locus_tag="ACHKYO_RS09915"
                     /old_locus_tag="pgaptmp_001983"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HGU4428839.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            29705..30322
                     /gene="dmsB"
                     /locus_tag="ACHKYO_RS09920"
                     /old_locus_tag="pgaptmp_001984"
     CDS             29705..30322
                     /gene="dmsB"
                     /locus_tag="ACHKYO_RS09920"
                     /old_locus_tag="pgaptmp_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HGU4428840.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            30324..31187
                     /gene="dmsC"
                     /locus_tag="ACHKYO_RS09925"
                     /old_locus_tag="pgaptmp_001985"
     CDS             30324..31187
                     /gene="dmsC"
                     /locus_tag="ACHKYO_RS09925"
                     /old_locus_tag="pgaptmp_001985"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HGU4428841.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(31223..31849)
                     /gene="ycaC"
                     /locus_tag="ACHKYO_RS09930"
                     /old_locus_tag="pgaptmp_001986"
     CDS             complement(31223..31849)
                     /gene="ycaC"
                     /locus_tag="ACHKYO_RS09930"
                     /old_locus_tag="pgaptmp_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HGU4428842.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     HLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            32164..33312
                     /gene="ycaD"
                     /locus_tag="ACHKYO_RS09935"
                     /old_locus_tag="pgaptmp_001987"
     CDS             32164..33312
                     /gene="ycaD"
                     /locus_tag="ACHKYO_RS09935"
                     /old_locus_tag="pgaptmp_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4428843.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            33522..34952
                     /gene="ycaM"
                     /locus_tag="ACHKYO_RS09940"
                     /old_locus_tag="pgaptmp_001988"
     CDS             33522..34952
                     /gene="ycaM"
                     /locus_tag="ACHKYO_RS09940"
                     /old_locus_tag="pgaptmp_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU4428844.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(34953..35861)
                     /gene="ycaN"
                     /locus_tag="ACHKYO_RS09945"
                     /old_locus_tag="pgaptmp_001989"
     CDS             complement(34953..35861)
                     /gene="ycaN"
                     /locus_tag="ACHKYO_RS09945"
                     /old_locus_tag="pgaptmp_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4428845.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            35961..36551
                     /gene="ycaK"
                     /locus_tag="ACHKYO_RS09950"
                     /old_locus_tag="pgaptmp_001990"
     CDS             35961..36551
                     /gene="ycaK"
                     /locus_tag="ACHKYO_RS09950"
                     /old_locus_tag="pgaptmp_001990"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HGU4428846.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPFWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(36633..37373)
                     /gene="pflA"
                     /locus_tag="ACHKYO_RS09955"
                     /old_locus_tag="pgaptmp_001991"
     CDS             complement(36633..37373)
                     /gene="pflA"
                     /locus_tag="ACHKYO_RS09955"
                     /old_locus_tag="pgaptmp_001991"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HGU4428847.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(37565..39847)
                     /gene="pflB"
                     /locus_tag="ACHKYO_RS09960"
                     /old_locus_tag="pgaptmp_001992"
     CDS             complement(37565..39847)
                     /gene="pflB"
                     /locus_tag="ACHKYO_RS09960"
                     /old_locus_tag="pgaptmp_001992"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU4428848.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(39902..40759)
                     /gene="focA"
                     /locus_tag="ACHKYO_RS09965"
                     /old_locus_tag="pgaptmp_001993"
     CDS             complement(39902..40759)
                     /gene="focA"
                     /locus_tag="ACHKYO_RS09965"
                     /old_locus_tag="pgaptmp_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HGU4428849.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT        10281 a         9975 c        10822 g         9912 t
ORIGIN      
        1 acgcttaacg cggggataaa agggattttt catgcaactt taagggagat tgatttagcg
       61 caattttggc ggcagggatc taccgccaga gaggtattac gcagagaaaa aggcgatgag
      121 gatcggcact aacaggctaa gaataaaacc gtgaacaatt gccgccggga ccatatccag
      181 cccgccagta cgttgaagaa cgggcagggt gaaatccatt gatgtggcac cgcataagcc
      241 cagtgcagta gagcggctgc ggcgaatcag cccagggatc aacataatag caatcagttc
      301 acgggccaga tcattaaaaa acgccgcgct cccgattacc ggaccaaaag attcggtcaa
      361 taaaataccg gaaagagaat accagccgaa accggaggcc attgccagcg cggtattgat
      421 ggggagatca agaataaagg cgttaattaa accaccaatt aatgaactga caaccaccac
      481 cacggcgaca atcattcccc ggcgattaag gacaatctgc tttaaggtca tgccattatt
      541 gcgcaactga ataccaacga ggaaaagtag caaaattaac gtgtattcac tggcttcggt
      601 cgtgtgttgt aagaaagcca gtccactcag accaatggca aaaccaatca ctactacgcc
      661 gcacagtttt agcgactcca gcgccatcgc aatacgcgac gggagttttt cttgctgatg
      721 gtggttgcgc cacggcaggc ctcgctccag ccacatcagg gcggcaatat tacacagtaa
      781 aataacggta atactgacgg cagaataatg cagaatcgcc aacaggttac tggcgaggtt
      841 atcgagaaac gccagactga tacccataaa aaagagaata aggtaaacca tccagcttaa
      901 tagctgatta ataactttta acgcagcttg ttggcgaagc ggaatgaggt aacccacaat
      961 caggggaacc agaatgatta acagcccaga aaacatgaaa acccagtcct tgcaaagatg
     1021 aagtcgaaat gcgctatgac acactactga aagcggaagg acgagtaaag ttgcaattaa
     1081 aaggaaatgt tatgcataag gagcagtaga gtattcgttt tcatttaaag atattcttgc
     1141 gctttaatta caaactgcac cgatgttggt ggcgtcaaaa tcgccgaggc gttccctgaa
     1201 ggccggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat gttacctcgc
     1261 gcccgacccg gtagccgtaa gggataagtc gagggcaccg cgcagcggcg attttgttcg
     1321 ccagagcccg ggggtgcatg gggcggcggc gattggccgc cccctgcgcg ctccttgcgc
     1381 cagtggcaat atgtggctta gctcatgaaa ggagcgcaac aagatgatga atcaacatat
     1441 aacaacatct taaaaaaagg cctgacatta cgccaggcct tctgcgttaa ttaatcacgc
     1501 ttttccagca gggtccggta aatcagacca ccgataatgc cgccgacaat tggcaccacc
     1561 cagaagaacc acagttgttc taatgcccag ccgccctgga agatagcaac cgcggtgctg
     1621 cgcgccgggt taacagaagt gttagtcacc ggaatactaa ttaagtgaat cagggttaag
     1681 gccagaccaa tagcgatcgg cgcaaaacct gccggcgcga atttgtcggt tgcgccgtgg
     1741 atcaccaaca ggaaacctgc actcaatacc agttcaacta ccagcgcgga aagcatggaa
     1801 taaccgcctg gtgaatgctc gccataaccg ttagaagcaa aaccgctggc tgccgcgtca
     1861 aaacccgttt taccactggc aattaaatac agcagcgccg ctgcaacaat accgccgaca
     1921 acctgggcaa ttacgtagcc aacgacttct tttgccggaa aacgtccgcc agcccataaa
     1981 ccaatagtga ccgccgggtt aaaatgacca ccagaaatat gaccaacagc aaaggccatc
     2041 gtcagaacgg tcagaccgaa cgccaacgcc acgccggcaa aaccaatgcc taattccgag
     2101 aagcctgcgg ccagttcagc actaccacag ccaccaaaaa caagccagaa agtaccaaaa
     2161 cattcagctg ctaattttct gaacatatcc acctcaatta aaaattgacc ctgtgaaaaa
     2221 tatggtcgtt ttatagggtc gtcgtaaaaa agtgacgacg gaaataatgc gcggctattt
     2281 taaaaacgaa ggcgagtcat tcaccagata aataaatcca gtaaatttga tttagggcaa
     2341 cagcgggttg ccccatatag tcatttgtct gattgacagt gtagtgcaag caaaagattt
     2401 aatcctttag gcgtaataaa aaataattta tcatgctaat tatttgattt tgttgttttt
     2461 gcagacttat cagcaagagg gagtataacg cgattattcg ctcatttttc agacatttgc
     2521 catgcttaaa tgtgatgtca tcacgtatta gcaaggcctt tcccgttata ctgccagcgt
     2581 aaaggataag tcacatattt ctggagggga tatgattctt gagcgcgttg aaattgtggg
     2641 ttttcgcggt atcaaccgtt tgtcgttgat gctcgaacaa aacaacgtcc tgattgggga
     2701 gaacgcgtgg ggtaaatcca gcttgctgga cgccttaacc ctgctgctat cgccagaatc
     2761 agatctctac cattttgagc gcgacgattt ctggttcccg ccgggagata tcaacgggcg
     2821 agaacatcat ctgcatatta ttttgacctt ccgcgaatcg ctgccaggcc gacatcgggt
     2881 tcgccgttat cggccgctgg aagcgtgctg gacgccatgc accgatggct atcaccgtat
     2941 tttttatcgt ctggaagggg agagtgcgga agacggcagc gtgatgacac tgcgcagttt
     3001 tctcgataaa gacggacatc cgattgatgt cgaggatatt aacgatcagg cacgccatct
     3061 ggtgcgttta atgccggtgc tgcgcttgcg tgatgcccgt tttatgcgcc gtattcgtaa
     3121 cggcacggtg ccaaatgttc ccaatgtgga agtcaccgcg cgccagctcg atttcctcgc
     3181 ccgtgagttg tcctcacatc cgcaaaatct ctctgatgga cagattcgtc agggactttc
     3241 cgcaatggtg cagttgcttg agcattattt ctctgagcag ggggctggac aggcgcgata
     3301 tcgtttaatg cggcggcggg ccagcaatga gcaacgaagc tggcgctatc tggacatcat
     3361 caaccggatg attgaccgac ctggagggcg ctcgtatcgg gttattttgc tcggcctgtt
     3421 tgctacttta ttgcaggcaa aaggcacatt gagactggat aaagacgcgc gtccgttatt
     3481 gctaattgaa gatccagaaa cccgcctgca tccgattatg ctttccgttg cctggcatct
     3541 gttgaatctt ttgcctttac agcgtattgc taccaccaac tctggcgagc tgctttcgtt
     3601 aactccagtg gaacatgttt gccgcctggt gcgtgaatct tctcgtgttg ccgcctggcg
     3661 tctggggcca agtggcttga gcaccgaaga cagccgccgc atctcttttc acattcgttt
     3721 taatcgtccg tcatcgctgt ttgctcgctg ctggttgctg gtggaagggg aaacggaaac
     3781 ctgggttatc aatgaactgg cgcgtcagtg cggacatcat tttgatgccg aagggatcaa
     3841 ggtcattgag tttgcccagt ccgggctaaa gccactggtt aaatttgccc gccgaatggg
     3901 gattgaatgg catgtactgg tcgatggcga tgaagcaggg aagaaatatg ccgctacggt
     3961 acgcagcctg ttgaataacg atcgggaagc cgaacgagaa catttaacgg cgttaccggc
     4021 gctggatatg gaacatttta tgtatcgcca gggattttcc gatgtgttcc accgcgtggc
     4081 gcaaatcccg gaaaatgtac cgatgaatct gcgcaaaatt atctcgaaag cgatccatcg
     4141 ctcttccaaa cccgatcttg ccattgaagt ggcaatggag gccggacgtc gtggtgtgga
     4201 ctccgtaccg acgctgctga aaaaaatgtt ctcacgcgtg ctgtggctgg cgcgcggtcg
     4261 cgcggattaa ccgcgaaaca tcgtggccat ttgtggctga atagcgtcga gcatctcata
     4321 gcgccgacgg tattcagccc gttttttact ggcgatttcg gcaatctctt ttcgtgctat
     4381 ctgtgctgga aggcggtaat ggcgttcagc atcacatacg ccgccaaccg attcccagaa
     4441 agcgttgtaa tcggcatgga tcttgccttc tttatcacga taacgcaggc tgcggtaaat
     4501 atgcgtttca ttgctgacgg caataatctg ctctacctgc aaacgttggg caaacagaca
     4561 ggccgcttcc atcacgaggc gtttgggaaa tagcccgtgg caggctttcg tcgcattctg
     4621 gatttcctga tgtggaattt cccattttgc gccttgcagt ccgccaataa acatcgttct
     4681 tttcccctga tattcacaca gggtaaacgt gatctctgcc agaggaatac cttcgctgtt
     4741 gcgaaacagg attgtgctgt caccttcttt atccattgag atcatcatgg tcagctcaag
     4801 cgtgaactgc tcgccgtttt tgccttccag cttcgccagt tgcagcccgg gggtattcaa
     4861 atataagctg aattcttccg ccgacataca tccgcggagt aacgcataat ggtaacgtaa
     4921 cgcctccagc aattgcttac ggctaagatt cgccgcaagg taagggcgat gcagacgcac
     4981 aggcagtcgc ggttggcgcg ttaacaacac attgagatta ggccagtggg aaagttcgtt
     5041 catccactca acgcttaaac gcggcataat caacgagcgc agcaaaaatt tctggcgaaa
     5101 actacggcga tgccagaatt tacccggccg acactgtcca cgtgccagac taagaaaaag
     5161 tgacaggctg ctgagagatt cagatggcgt aaaggtccgt tcagttagct gcgacatatt
     5221 catgaaatca atggttatac atgacgtcga tttcaccatt gcgtatctta accaaacatc
     5281 aatagtgtga ttactaacgt aaattttatg gttttgttga tatttcgttg aagttaatga
     5341 cccggattgg catatggagt attcagaata tttatgaaaa agcggaaaac cgtgaagaag
     5401 cgttacgtta ttgcgctggt gatagtcatc gccggactga ttacgttgtg gagaattctt
     5461 aacgcacccg tgccgactta tcagacgctg attgtgcgcc ccggtgattt acagcaaagc
     5521 gtgctggcga ccggaaagct ggacgcgctg cgtaaggttg acgtgggcgc gcaggtcagc
     5581 ggtcagttga aaactctgtc ggtggcgatt ggcgataaag taaaaaaaga ccagctttta
     5641 ggggttattg atcctgaaca ggctgaaaac cagattaagg aggtcgaagc aacgctgatg
     5701 gagctacgtg cgcagcggca gcaggcggaa gcggagctga aactggcgcg ggtgacgtat
     5761 tcccgtcagc aacgtctggc acaaacgcag gctgtttcac agcaggatct cgacaccgcc
     5821 gcgacggaga tggctgtgaa acaggcgcaa attggcacca ttgacgcgca aatcaagcgc
     5881 aatcaggctt ctctcgatac ggctaaaacc aatctcgatt acactcgcat cgttgccccg
     5941 atggccgggg aagtcacgca aatcaccact ctgcaaggcc agacggtgat tgccgcacaa
     6001 caagcaccga acattctgac gctggcagat atgagcacca tgctggtaaa agcgcaggtt
     6061 tctgaagcgg atgtaatcca cctgaagccg gggcaaaaag cctggtttac ggtgcttggc
     6121 gatccactga cgcgctacga ggggcaaatc aaggatgtac taccgacgcc ggaaaaggtt
     6181 aacgacgcta ttttctatta cgcccgtttt gaagtcccca atcccaatgg tttgctgcgg
     6241 ctggatatga ctgcgcaagt gcatattcag ctcaccgatg tgaaaaatgt gctgacgatc
     6301 cctctgtcgg cgttaggcga tccggttggc gataatcgtt ataaagtcaa attgttgcgt
     6361 aatggtgaaa cacgcgagcg tgaagtgacg attggcgcac gtaacgatac cgatgttgag
     6421 attgtcaaag ggcttgaagc gggcgatgaa gtggtgattg gtgaggccaa accaggagct
     6481 gcacaatgac gcctttgctc gaattaaagg atattcgtcg cagctatcct gccggtgatg
     6541 agcaggttga ggtgctgaag ggcatcagcc tcgatattta tgcgggtgag atggtcgcga
     6601 ttgttggcgc ttcgggttcc ggtaaatcga ccctgatgaa tattctcggc tgtctggata
     6661 aggccaccag cggcacctat cgcgtcgccg gtcaggatgt tgccacgctg gacgccgatg
     6721 cgctggcgca actgcgccgc gagcatttcg gctttatttt ccagcgttac catttgcttt
     6781 cgcatttaac cgccgagcag aacgttgaag tacccgccgt ctatgctggt cttgagcgga
     6841 aacagcgact gcttcgtgcc caggagttgc tgcaacggct ggggctggaa gaccgtacag
     6901 agtattatcc ggcacagctt tcgggtggtc agcaacagcg cgtcagcatc gcgcgggcat
     6961 tgatgaacgg tggtcaggta attcttgccg atgaaccaac cggcgcactg gacagccatt
     7021 ctggcgaaga ggtgatggcg atcctgcatc agctgcgcga tcgtgggcat acggtgatta
     7081 tcgtcaccca cgatccgcag gtcgctgctc aggccgagcg ggtgatcgaa attcgcgacg
     7141 gcgaaattgt gcgcaatcct cccgccattg aaaaagtgaa tgttgctggc gggacggagc
     7201 ctgttgtcaa cacggtgtct ggctggcggc agtttgtcag cggttttaac gaggcgctga
     7261 cgatggcatg gcgggcactg gcagcgaata aaatgcgtac tttactgacc atgctgggga
     7321 ttattatcgg tattgcgtcg gtggtttcca ttgtcgtggt gggtgacgcc gccaaacaaa
     7381 tggtgctggc ggatattcgt tctataggta cgaatactat tgatgtctat cccgggaaag
     7441 attttggcga tgacgatccg caatatcagc aggcgctgaa gtacgacgac ttaatcgcca
     7501 tccaaaaaca accgtgggtc gcctcagcca cacctgccgt ctcgcaaaac ctgcgcctgc
     7561 gttataacaa tgttgatgtt gctgccagtg ccaatggcgt gagcggcgat tattttaatg
     7621 tctatggcat gaccttcagt gaaggaaaca cctttaatca ggagcagctg aacggtcgtg
     7681 cgcaggtcgt ggttctcgac agtaatactc gccgccagct tttcccccat aaagcagatg
     7741 tggttggcga ggtgattctg gtcggcaata tgcccgccag agtcattggt gtggcggaag
     7801 aaaaacagtc gatgtttggt agcagtaaag tgctgcgtgt ctggctacct tacagcacga
     7861 tgtccgggcg agttatgggc cagtcgtggc ttaactccat tactgtcagg gtaaaagaag
     7921 gatttgacag cgccgaggcg gaacagcaac tcacgcgttt actttcactg cgccacggta
     7981 agaaggattt ctttacctgg aacatggacg gcgtcttgaa aactgttgaa aagaccacac
     8041 gtactttaca actgtttctg acgctggtgg cggtgatttc gctggtggtg ggtggtattg
     8101 gtgtaatgaa tattatgctg gtgtcagtga ccgagcggac gcgggaaatt ggcattcgca
     8161 tggctgtagg tgcgcgagca agcgatgttt tgcaacagtt cctgatcgaa gccgtactgg
     8221 tttgcctggt cggtggcgcg ttgggaataa cactgtcact gttaattgct ttcaccttgc
     8281 agcttttctt acccggctgg gagattggtt tttcaccgtt ggcactgctg ctggcgtttc
     8341 tctgctcgac ggccaccggg attttatttg gctggttacc cgcacgaaat gcggcacgac
     8401 tggatccagt agatgctctg gcacgagagt aatttttgag ataaaaatgc cagccgatcg
     8461 ggctggcatt ttgcctttag gatgtacaca atgagacaga agagctatgc gactgccgct
     8521 tctacttcga cgggcacaat aacactggcg tgattgcctt ttggcccctg gtggacatca
     8581 aactgaacgg attgtccagc ttttagcgtt ctgtaaccat ccatctgaat ggtggaataa
     8641 tgagcgaaaa tatcttcgcc gccgccttca gggcagatga aaccaaaccc tttggcattg
     8701 ttgaaccact taacagtacc cttttccatg cttcgacatc cttcgcaaat cttatacaag
     8761 taagatggaa taaaccgggg tcagagaggg ggctgttcaa aacctcgcca actctagaaa
     8821 tacaatttag agaattaggg cgagccgtca agcatttgac aggggacagg gggcaggtat
     8881 gaatcaaaaa tttgaagcag ttaacgctat tgacaggaat gtgacagatg tcgctgatgc
     8941 caacgataga tgatagttat ctatcatgtg aagtagattg gtcaggcaaa taagctcttg
     9001 tcagcggcag ggcgttctgc cgataaccgt aaccgaagat gataactgac aatgggtaaa
     9061 acgaacgact ggctggactt tgatcaactg gcggaagaaa aagttcgcga cgcgctaaaa
     9121 ccgccatcta tgtataaagt gatattagtc aatgatgatt acactccgat ggagtttgtt
     9181 attgacgtgt tacaaaaatt cttttcttat gatgtagaac gtgcaacgca attgatgctc
     9241 gctgttcact accaggggaa ggccatttgc ggagtcttta ccgccgaggt tgcagaaacc
     9301 aaagtggcga tggtgaacaa gtacgcgagg gagaatgagc atccattgct gtgtacgcta
     9361 gaaaaagcct gaatgcaggc ataaaaattg ggggaggtgc ctatgctcaa tcaagaactg
     9421 gaactcagtt taaatatggc tttcgccaga gcgcgcgagc accgtcatga gtttatgacc
     9481 gtcgagcact tgttactggc gctgctcagt aacccatctg cccgggaggc gctggaagcg
     9541 tgttctgtgg atttggttgc gctccgtcag gaactggaag cctttattga acaaaccaca
     9601 cccgttctgc ctgccagtga agaggagcgc gacacacagc cgacgctgag ttttcagcgt
     9661 gtactgcaac gtgcggtctt ccatgtccag tcctccggtc gcaatgaggt aaccggtgca
     9721 aacgttctgg tcgctatctt tagcgaacag gagtcgcagg cggcatatct gttgcgtaaa
     9781 catgaagtca gccgtctcga tgtggtgaac tttatctctc atggcacgcg taaagacgag
     9841 ccgacacagt cttctgatcc tggcagccag ccaaacagcg aagaacaagc tggtggggag
     9901 gaacgtatgg agaatttcac gacgaacctg aatcagcttg cgcgcgtggg cggaatcgac
     9961 ccactgattg gtcgtgagaa ggagctggag cgtgctattc aggttctctg ccgtcgccgt
    10021 aaaaacaacc cgctgctggt gggggaatct ggtgtcggta aaaccgcgat tgcggaaggt
    10081 cttgcctggc gaattgttca gggcgatgtg ccggaagtga tggctgactg tacgatttac
    10141 tctctcgata tcggttctct gttagcgggc actaaatatc gcggcgactt tgaaaaacgt
    10201 tttaaagcgt tgctcaagca gctggagcag gacactaaca gcatcctgtt tattgatgag
    10261 atccacacca ttatcggtgc gggtgcagcg tctggtggcc aggttgatgc ggctaacctg
    10321 atcaaaccgt tgctctccag cggtaaaatt cgcgtaattg gttcgacaac ctatcaggag
    10381 ttcagcaaca ttttcgagaa agaccgtgct ctggcgcgtc gcttccagaa aattgatatt
    10441 actgaaccgt cgatcgaaga aactgttcaa atcatcaatg gcctgaaacc gaagtatgaa
    10501 gcgcaccacg acgtgcgtta taccgcaaaa gcggtgcgtg cagcggtaga gctggcggtg
    10561 aaatacatta acgatcgtca tctgccggat aaagccattg acgttatcga cgaagcgggc
    10621 gctcgcgcac gcctgatgcc ggtaagcaaa cgcaagaaaa ccgttaatgt ggcggatatt
    10681 gagtccgtgg tggcccgtat tgcgcgcatt ccagagaaga gtgtttctca gagtgaccgc
    10741 gataccctga aaaacctcgg cgatcgcctg aaaatgctgg tcttcggtca ggataaagcc
    10801 attgaggcgc tgactgaagc cattaagatg gcgcgtgcag gtttaggtca cgaacataaa
    10861 ccggttggtt cgttcctgtt tgccggccct accggggtcg ggaaaacaga ggtgacggta
    10921 cagctttcga aagcgttggg cattgagctg ctgcgctttg atatgtccga gtatatggaa
    10981 cgccataccg tcagccgtct gattggtgcg cctccgggat acgttggttt tgatcagggc
    11041 ggtttgctga ctgatgcggt catcaagcat ccacatgcgg ttctgctgct ggacgaaatc
    11101 gagaaagcgc accctgacgt gttcaatatt ctgttgcagg tgatggacaa cggtacgctg
    11161 accgataaca acggacgcaa agcggacttc cgtaacgtgg tgctggtgat gaccaccaac
    11221 gccggggtac gtgaaactga gcgtaaatcc attggtctta tccaccagga taacagcacc
    11281 gatgcgatgg aagagatcaa gaagatcttt acaccggaat tccgtaaccg tctcgacaac
    11341 attatctggt ttgatcatct gtcaaccgac gtgatccatc aggtggtgga taaattcatc
    11401 gtcgagttgc aggttcagct ggatcagaaa ggtgtttctc tggaagtgag ccaggaagcg
    11461 cgtaactggc tggccgagaa aggttacgac cgggcaatgg gcgcacgtcc gatggcgcgt
    11521 gtcatccagg acaacctgaa aaaaccgctc gccaacgaac tgctgtttgg ttcgctggtg
    11581 gacggcggtc aggtcaccgt cgcgctggat aaagagaaaa atgagctgac ttacggattc
    11641 cagagtgcac aaaagcacaa ggcggaagca gcgcattaat ctgattgtta ggtaggttgg
    11701 tcaagtccgt aatctcgaaa gaggttacgg actttttgtt tatggggtgg aggaggttca
    11761 gacccttttt ttaatgatga tggtaagttg ttgataatta gtgctgcggg aaggtaagga
    11821 taaaaaaggg tgctgcagga gaatgggatg gttttgcttt attaacaacg ggctaaacgt
    11881 gtagtatttg agttcactgc cgtacaggca gcttagaaat cgacggggtg cggtaaaacc
    11941 tttgcgaacg cgttcactgc cgtacagaca gataaaatgc gaaaaaaaag ctcgcacttt
    12001 cgtacgagct cttctttaaa tatggcggtg agggggggat tcgaaccccc gatacgttgc
    12061 cgtatacaca ctttccaggc gtgctccttc agccactcgg acacctcacc aaattgtttt
    12121 gctaccaaac ctcatgggtg gcaacggggc gctactatag ggagttggag taaaacggtc
    12181 aagaagaatt ttaatgataa ttattgtttg ctcatactgt aaacaagttg tgcagtatat
    12241 ctacatcgag acaagttacg gacttatact tccaaagtac ttcatacata tcacaaaata
    12301 aaaaggccgg ttaaaccgac cttttactcg ttctttctct tcgcccatca ggcggtaaaa
    12361 caatcagcga ctacggaaga caatgcggcc tttgctcagg tcgtacgggg tcagttcaac
    12421 agtcactttg tcgcccgtca ggatgcggat gtagtttttg cgcattttac cggagatgtg
    12481 tgcagtaacc acgtgaccgt tttctaactc tacgcggaac atggtattag gcaacgtttc
    12541 aagaacggta ccttgcattt caatattgtc ttctttggcc atctaatcct ctggggtatc
    12601 actaccgtaa tttgaaccgg caagataatg ccgaagttct gtaaataagt aaagatttgc
    12661 gcgctaaatc gcaacaaaca ggttcggcac attactccga aaacacacgg ctaagccgca
    12721 ccaaaagcgc aacgtataag ggagcggtga gataaacgat gggcgttacc tgacgcgaaa
    12781 aattccttat cggcagcggg gtaatgagcg taaccaactc tgcgaccgca attataacac
    12841 tctggggaga aatgtgccga aaacattcat tcttgtggtg aaaacaagca tcgtggtacc
    12901 cagaaattat tcggcaatcg tccgaggcgc atttgattga gataattaag gtaatcccgg
    12961 cggggaattt cgcaggcacc aagcgatgct gtgtgatcgt taaggacctg gcagtcgata
    13021 agcttaccgc catgaccgat aaattcctca cagaatacca gaagcgccgt tttagacgca
    13081 ttttccatcc ggctgaacat ggactcgcca caaaatagcg ttccctgggc cacgccgtac
    13141 ataccgccga caagctcatc ttcacgccag acttcaatgg agtgggcatg gccgagttcg
    13201 tgaaggcgat ggtaggcttc gaccacgcca cgcgtgatcc aggttccttc ttcgcgatcg
    13261 ctggcacagc cttcaatgac ctgaccaaaa gcgtaattca tcgtgacacg atagggcgag
    13321 cgtttatgaa atcgcttcat actacggctg atatgcagtg attctggcca tagcaccgcg
    13381 cggggatcgg gcgaccacca gaggatgggg tcgcctggag aaaaccacgg aaaaatacca
    13441 cgctggtaag ccattaacag gcgcgcaggg ctaagatcgc ccccaagtgc cagcaggccg
    13501 ttaggctcac gtaatgcgcc ttccggggaa gggaaggcta ttgaatggcg agaaagctga
    13561 accaggcgca tgaccgcaaa actccacgca agtcggatcg ttcaataata gcttacaaac
    13621 cctgcttgaa ctggtaataa cgcccctgtc tggcaagcag ctctgcgtga gtaccttgct
    13681 caataatttg cccgttgtcc atcactatta tttgttggaa acgagagagt ccgcgaagtc
    13741 gatgggtgac cattaacacc gttttctcac tcatcatttc tgcaagcaat tcaaggatct
    13801 ggctttcggt tgtggcatct aagccttcgg taggttcatc cagcaacacc agtggcgcat
    13861 catgtaacag cgcacgggcg atagccagac ggcgcagttc accaccggag agctggcgtc
    13921 cgccttcacc taaccaactg ttgagacctg catcctcgag cagcttctcc agaccaacgc
    13981 gacgcaagat ctccgacaga gcctcatcac tactgccagg cgaggcgagt aacagattat
    14041 cacgcagcgt ggcgctaaac agatgcactc gctgaggaac aacgctgatg gtctgtcgta
    14101 gagccgcttc attcaggctg gctatggggc tatcgttaag caaaatctcg ccctgttgcg
    14161 ggtcccatgc gcgggtcagc agttgtaaca gtgttgattt gccgcatccg gttcgcccga
    14221 gaatcgctat atgttccccg gcgtttacct gaagagaaat ccctttaagt gcctgttggg
    14281 attgctccgg ataagtgaac tgaacatccc gtaatgtcag cgaaacgcga tcggcaacac
    14341 gagtttgggg atcagggaag gtgacctccg gtttttgatc cgttaagtca gagatacgta
    14401 cggcagaggc aatgacttgc cccagatgct gaaatgcacc cgttactggt gccagtgctt
    14461 caaacgcggc taacgcgcag aagacaaaca gggcaattaa cgcgccgggt tgagcattgc
    14521 cgccaacgcc gccagacgcc atccacagca tcaggatcac cgctaacgcg ccaatgagca
    14581 gcattatcgc ttgcgacaat gcggtcagtt cagattgacg gcgttgcgct tccagccatt
    14641 gaatttctgt attctctagt tgcgtgcgat aacgatcgct ggcaccaaaa atggtcagct
    14701 cagcttgccc ttgcagccag gccgtcagtt gttggcgata ctgcccgcga agatgagtca
    14761 gattttgccc ggtgcttttt cccgcacgat aaaacagcgg tggcatcagg aaaagcgtca
    14821 gtaacataat gccgccaagc gtaaaggcga gggtgaaatc aaggaaactt aacccgattg
    14881 tcaccaccat aatcaccaca aaagcgccca ccagcggcga gataacgcgc aggtaaagat
    14941 gatcgagcgt atcaacatcc gccaccacgc ggttgagcaa ttcgccctga cgatagcgtg
    15001 ccagtccggc aggggagagg ggcagcaatt tgctgaaggt gtaaatgcgc agatgctgca
    15061 acacgcggaa agtcgcgtcg tgacttacca gacgttcaaa ataacgcccg gcagtacggg
    15121 tgattgctgc gccacgcacg cccgcagcgg gtagcatata gttgaagctg tacagtccgg
    15181 caacccccgc aaccgctgag gccgagagga accagccgga aagtgtcaac agaccgatac
    15241 tggcgagcag cgtgacaatt gccagcacga taccaagact taacatccat ttatgacgtt
    15301 tatacagtgc cagatagggt agcaaagcgc gcatttaaat ctcctcctga cgatgggcca
    15361 gtaatgtggc gaatgggcca ccagccacac ttaattccgc gtaacgtcct tgctcaataa
    15421 tctgaccatc ctgcattacc caaatgacat cccagtcagc aagatcttct aactggtggg
    15481 tgaccattaa cgttgtctgg cgcagagagg cggcattcag cgcctccatt acgcgctgtt
    15541 cactgtgagc atcaaggctg gcagcgggtt catccaatag taatagcgag cagggattta
    15601 gtaacgcacg ggccaccgcc acgcgctgcg cctgccccac ggaaaggcgg gcagcctggt
    15661 cgccaacagg cgtatcaacg ccttgtggca ggagcggtag aaactcgctg acccaggcgt
    15721 tatccagcgc tgcttgtaat tcatgttcgc tggcatcagg tcgcgccagt agtacgttat
    15781 cccgtaatgt tgctgccggt aattgtggat tttgcccaac ccaggagaga tgtttacgcc
    15841 atgattctgg tgataaatcg cgtaattcta tcccgttgat tcgtagcgat ccctgatatg
    15901 agagaaaacc agaaagcgcg ttcaacagtg aacttttacc tgaaccgctg cgaccaacca
    15961 acacggcacg ttggcctgct ggcaaagtaa agttcagcgg tccggccagc gttttacctt
    16021 ccggcgacgt gataaacaga tcctcggcct caatggtcac cggatcggtc gatgctaatt
    16081 ccacctcgcc acgctgcgga tgggcgagcg gggtttccat aaacgttttc agactgtcag
    16141 ctgcaccaac agcctgggct ttagcatgat aaaacgtacc gagatcgcgt aatggctgga
    16201 aaaactctgg cgcaaggatc agggccagaa aacccgcagc cagcgtcacg ccagtatcgt
    16261 agtgaccaaa atccagctcg ccgagatagg aaaaaccaaa gtagaccgcc accagagcaa
    16321 ttgacagcga ggtaaaaaat tcgagaatgc cggaggataa aaatgccagc cgtagcactt
    16381 ccattgtccg ttggcggaaa tcttccgaag cagaacgaat actttcaatt tcagcttcac
    16441 cacgaccaaa aatacgcaat gtttccatgc cgcgcaggcg atcgaggaaa tgcccactta
    16501 agcgagcaag agcgagaaag ttacgtcggt tagcatcggc agcccccatc ccaaccagcg
    16561 ccataaacaa cggaattaac ggtgcagtgc ccagcagaat gagcgccgca gcccagttag
    16621 aggggaagat cgccaccaca atcagcaacg gcaccgacac tgccagcgcc atttgcggca
    16681 ggtagcgtgc atagtaatca tgcatatcgt caatttgctc gagtaccagc gtcgcccagc
    16741 tccccgcagg tttaccctga atccacgctg gccctgcttg ttgcagacgg tcgagaacct
    16801 gacggcggat ggcaaagcgg atatgctgcc cggcgtgata acccacccgt tcgcgtaacc
    16861 agaccaccca tgcgcgcagt acaaaggtca gaaccagtaa cgtaaaggga agcagcagag
    16921 cttcacgggg aatattctcc ataatcatat gttgcagaat tcgcgccatg aaccaggcct
    16981 gggcaatgat caatatgccg ctcacaaagc ccagcagacg agaaatattc agccaacgtt
    17041 gggagatgac gctttgctgt tttaaccagc gggttaactc tttttgacgg gatttattca
    17101 ttgcacgctt agcaggtgag ttatcagaat tatttgcaga gcaatgttac aacgaggaaa
    17161 aaataaaggc gacccatagt cgcatggtgt cgccttcttt acttttgtta ctgatttgta
    17221 aaattatttt gcgtcagcta aaccatcgag gtagcgttcc gcatcaagtg ctgccatgca
    17281 gcctgtacca gcagatgtaa tagcctggcg ataaatgtga tccatcacgt cgcctgcggc
    17341 aaagacgcca gggatgctgg tctgggtggc attaccatga atacccgact gtactttgat
    17401 gtagccgttt tccagttcca gctgcccttc gaaaatggca gtattcgggc tgtgaccgat
    17461 agcaacaaac agaccggcaa cgtcgagtga ctcgatgtta tcgctgtttt gcgtatcgcg
    17521 cagacgaacg ccagtgacgc ccatctgatc gccggtcacc tcttccagcg tacggttggt
    17581 gtgtagaatg atgttgccgt tctccacttt atccatcaga cgcttaataa ggattttttc
    17641 cgcgcggaaa ccgtcacggc ggtgaatcag atgcacttcc gaagcgatgt tagacagata
    17701 cagcgcctct tcaaccgcgg tattgccgcc gccgatgacc gcaactttct ggttgcgata
    17761 gaagaaaccg tcgcaggtag cacaagcaga aactccacgg cctttaaagg cttcttcaga
    17821 tggcaggccg agatagcgtg cagaagctcc agtggcaata atcagcgcgt cgcaagtgta
    17881 ttcgccgcta tcgccagtca gacggaacgg tcgattctgc aaatcaactt tgttgatgtg
    17941 atcaaagatg atttcagttt caaatttggt ggcatgttcg tgcatgcgct ccattaataa
    18001 cggaccggtc agatcgtttg gatcgccagg ccagttttcc acttccgtgg tggtggtcag
    18061 ttggccgcct ttttccatgc cggtaatcag cacaggttgc aggttggcgc gcgctgcata
    18121 gacagcagcg gtgtatcccg ccgggcctga acccaggata agcagtttac tgtgtttggt
    18181 cgtgcccatg agatccccat agttgttggc agacaatggg caggattgta gggaatttat
    18241 agacgtaaaa aaagagtatg acgattttgt taacaatttg tgcaatcggc agcatcgata
    18301 agcaggtcaa attctcccgt cattatcacc tctgctactt aaatttcccg ctttataagc
    18361 cgattaaatg atgaataaac gcccctgtta atgaatatct ggcatgttgt actaaaaatc
    18421 gatgttttgc tttgacaatc ccctggtgtt ttgcgaaaac attcgaggaa gaaaaaaaac
    18481 agtattctta tatgcgcata accatgcatg taaataccat gtttaccgtg ctagtgaaat
    18541 ctacgtatgg cgtggacaga cgccattcgt gatgtcgata gctgccacaa ggcaacggtc
    18601 ttctcaccgt agacccaggc attgcgcgcc gtgaatcttc atgatttcgg tctatcgtga
    18661 cgggtagcga ctctgaacag tgatgtttca gagtcagaca ggagtaggga aggaatacag
    18721 agagacaata ataatggtag atagcaagaa gcgccctggc aaagatctcg accgtatcga
    18781 tcgtaacatt cttaatgagt tgcaaaagga tgggcgtatt tctaacgtcg agctttctaa
    18841 acgtgtggga ctttccccaa cgccgtgcct tgagcgtgtg cgtcggctgg aaagacaagg
    18901 gtttattcag ggctatacgg cgctgctgaa cccccattat ctggatgcat cacttctggt
    18961 attcgttgag attactctga atcgtggcgc accggatgtg tttgaacaat tcaataccgc
    19021 tgtacaaaaa cttgaagaaa ttcaggagtg tcatttagta tccggtgatt tcgactacct
    19081 gttgaaaaca cgcgtgccgg atatgtcagc ttaccgtaag ttgctggggg aaaccctgct
    19141 gcgtctgcct ggcgtcaatg acacacggac atacgttgtt atggaagaag tcaagcagag
    19201 taatcgtctg gttattaaga cgcgctaaca cggaacaggt gcaaaatcgg cgtattttga
    19261 ttacactcct gttaatccat acagcaacag tactggggta acctggtact gttgtccgtt
    19321 ttagcatcgg gcaggaaaag cctgtaacct ggagagcctt tcttgagcca ggaatacact
    19381 gaagacaaag aagtcacatt gacaaagtta agtagcggcc gccgccttct ggaggcgttg
    19441 ctgatcctta ttgtcctgtt tgccgtctgg ttgatggctg ccttactaag ctttaaccct
    19501 tcggacccca gctggtcgca aacggcctgg catgaaccta tccataattt aggtgggatg
    19561 cccggtgcgt ggctggcaga tacgctgttc tttatttttg gcgtgatggc ttacaccatt
    19621 cccgtcatta ttgtcggcgg ttgttggttt gcctggcgtc atcagtccag cgacgaatac
    19681 attgattatt ttgccgtttc gctacgcatc attggcgttt tggcgctcat ccttacctcc
    19741 tgtggtctgg cggcaatcaa cgctgacgat atctggtatt ttgcctccgg tggcgtcatt
    19801 ggcagcttac taagtactac gctacaacca ctgctacaca gtagcggggg aactattgcg
    19861 ctgctctgcg tttgggcagc gggcctgacg ctgttcaccg gttggtcatg ggtgaccatt
    19921 gctgaaaaac tcggcggctg gattttaaac attctcacct tcgccagtaa tcgtacccgt
    19981 cgcgatgata cctgggtcga tgaagatgaa tatgaagatg acgaagagta tgaagatgaa
    20041 aaccacggca aacagcatga atcacgccgt gctcgtattc ttcgcggcgc gctagcgcgt
    20101 cgtaaacggt tggcggaaaa attcattaat ccgatggggc ggcaaacaga cgctgcgttg
    20161 ttctccggta agcggatgga tgatgacgaa gagattacct acactgcacg cggtgtggct
    20221 gccgaccctg acgacgtcct attttcgggc aatcgtgcaa cgcagccaga atatgacgaa
    20281 tacgatccat tattaaacgg tgcgccaatt accgaacctg tcgctgtagc agctgctgct
    20341 accacggcga cacaaagctg ggctgcgccg gttgaacctg tgactcagac gccgcctgtt
    20401 gcctctgttg acgttccacc ttcgcaacct acagtagcct ggcagcctgt accgggtcca
    20461 caaacgggag agccggttat tgctcctgca ccggaaggtt acccacagca gccacaatat
    20521 gcgcagcctg cagtgcaata taatgagccg ctgcaacaac cagtacagcc acagcagccg
    20581 tattatgcac ctgcagctga acaacctgcg caacaacctt actacgctcc tgcggcggaa
    20641 caacctgttc agcaaccgta ttatgcaact gcgccggaac agcctgcgca acagccgtat
    20701 tatgcccctg cgccagaaca accggtggca ggtaacgcct ggcaagccga agagcagcaa
    20761 tccacttttg ctccacagtc tacataccag actgagcaaa cttatcagca gccagccgct
    20821 caggagccgt tgtaccaaca gccgcaaccc gttgaacagc agcctgttgt ggagcctgaa
    20881 cccgttgtag aagagacaaa acccgcgcgt ccgccgcttt actattttga agaagtggaa
    20941 gagaagcgag cccgtgaacg tgaacaactt gcggcctggt atcaaccgat tccagaaccg
    21001 gttaaagaac cagaaccgat caaatcttcg ttgaaagcgc cttctgttgc agcagtacct
    21061 ccagtagaag ccgctgccgc tgtttctccg ctggcatctg gcgtgaaaaa agcgacactg
    21121 gcgacggggg ctgccgcaac cgttgccgcg ccagtcttca gtctggcaaa tagcggtgga
    21181 ccgcgtccgc aagtcaaaga ggggattggc ccgcagttgc cacgaccgaa acgtatccgc
    21241 gtgccaactc gtcgtgaact ggcgtcttac ggtattaagc tgccctcaca gcgtgcggcg
    21301 gaagagaaag cccgtgaagc ccagcgcaat cagtatgatt ctggcgatca gtataacgat
    21361 gatgaaatcg atgcgatgca gcaggatgaa ctggcacgtc agttcgccca gacacagcag
    21421 caactctatg gcgaacagta tcaacatgat gtgcccgtaa acgcagaaga tgcagatgct
    21481 gcggcagagg ctgaactggc tcgtcagttt gcgcaaactc aacaacaacg ttattccggc
    21541 gaacaaccgg ctggggcgaa tccgttctcg ctggatgatt ttgaattttc gccaatgaaa
    21601 gcgttgctgg atgatggtcc acacgaaccg ttgtttacgc caattgttga acctgtacag
    21661 cagccgcaac aaccggttgc accgcagcag cagtatcagc agccacaaca gccggttgcg
    21721 ccacagcagc aatatcagca gccgcaacag ccggttgcgc cgcagccgca gtatcagcag
    21781 ccacaacagc aggttgcgcc gcagccacaa tatcagcagc cgcaacaacc ggttgcgcca
    21841 cagccgcaat atcagcagcc gcaacaacca gttgcgccgc agcagcagta tcagcagccg
    21901 caacaaccgg ttgcaccgca gccgcagtat caacagccgc aacaaccggt tgcaccgcag
    21961 cagcaggata ccctgcttca tccgctgttg atgcgtaatg gcgacagccg tccgttgcat
    22021 aaaccgacga cgccgctgcc ttctctggat ttgctgacac cgccgccgag cgaagtggag
    22081 ccggtagata cctttgcgct tgaacaaatg gctcgcctgg tggaagcgcg tctggctgat
    22141 ttccgtatta aagccgatgt cgtcaattac tctccggggc cggttatcac tcgctttgaa
    22201 cttaacctgg ctcctggcgt aaaagcggcg cgcatttcta acttgtcacg ggaccttgcc
    22261 cgttcacttt cgacggtggc ggtgcgtgtc gttgaagtta ttcctggcaa accctatgta
    22321 ggtctggagt taccaaataa aaaacgacaa accgtttatc tgcgcgaagt tttggataac
    22381 gccaaattcc gcgataatcc gtcgccatta accgtggtgc tgggtaaaga tatcgccggt
    22441 gagccggtgg ttgccgatct ggcgaaaatg ccgcacttgt tggttgcggg gactaccggt
    22501 tccggtaaat ctgtcggtgt gaacgcgatg atcctgagca tgctttataa agcacagcca
    22561 gaagatgtgc gtttcatcat gatcgacccg aaaatgctgg agctttcggt ttatgaaggc
    22621 attccgcatc tgttaacgga agtcgttact gatatgaaag atgccgccaa cgcgctgcgc
    22681 tggtgtgtta acgagatgga gcgtcgttat aaactgatgt ctgcgctggg tgtgcgtaat
    22741 ctggcgggtt ataacgaaaa aattgctgaa gccgatcgca tgatgcgtcc gattccagac
    22801 ccgtactgga agccgggtga cagtatggat gcccagcatc cggtgctgaa aaaagaacca
    22861 tacattgtgg tgttggttga cgaatttgcc gacctgatga tgacggtagg taaaaaagtg
    22921 gaagagctga tagcacgtct ggcgcaaaaa gcccgtgccg cgggtatcca cctcgtactg
    22981 gcaactcagc gtccatcggt tgatgttatt actggtctga ttaaagcgaa tattccgacc
    23041 cgtatcgcct ttaccgtatc cagtaagatt gactcacgta ccattcttga tcaggctggc
    23101 gcggaatcac tgctggggat gggggatatg ctctactctg ggccgaactc cacgttgccg
    23161 gtacgtgtcc atggtgcttt tgttcgcgat caggaagttc atgccgtggt gcaggactgg
    23221 aaagcgcgtg gtcgcccaca gtatgttgat ggcatcactt ccgacagtga aagcgaaggt
    23281 ggcgcgggcg gtttcgatgg cgctgaagaa ctggatccgt tgttcgatca ggcggtgcag
    23341 tttgtcactg aaaaacgcaa agcgtcaatt tctggcgtac agcgtcagtt ccgcattggt
    23401 tataaccgtg cagcgcgtat tatcgaacag atggaagcgc aggggattgt cagcgaacag
    23461 gggcacaacg gtaatcgtga agtgctggcc ccaccgccgt ttgactaatt aatgcattgc
    23521 cggataaggc gcggtagcgt cgcatccggc actctatcaa ctgaaaattc agtattttct
    23581 tctttcctca agctgattat tagcctggaa tagagagtag agggaactcc cgatcgggag
    23641 tgacgtaatt tgaggaataa tgatgaaaaa aattgccatc acctgtgcat tactctcaag
    23701 cttagtagca agcagcgttt gggctgatgc cgcaagcgat ctgaaaagcc gcctggataa
    23761 agtcagcagc ttccacgcca gcttcacaca aaaagtgact gacggtagcg gcgcggcggt
    23821 gcaggaaggt cagggcgatc tgtgggtgaa acgtccaaac ttattcaact ggcatatgac
    23881 acaacctgat gaaagcattc tggtttctga cggtaaaaca ctgtggttct ataacccgtt
    23941 cgttgagcaa gctacggcaa cctggctgaa agatgccacc ggtaatacgc cgtttatgct
    24001 gattgcccgc aaccagtcca gcgactggca gcagtacaat atcaaacaga atggcgatga
    24061 ctttgtcctg acgccgaaag ccagcaatgg caatctgaag cagttcacca ttaacgtggg
    24121 acgtgatggc acaatccatc agtttagcgc ggtggagcag gacgatcagc gcagcagtta
    24181 tcaactgaaa tcccagcaaa atggggctgt ggatgcagcg aaatttacct tcaccccgcc
    24241 gcaaggcgtc acggtagatg atcaacgtaa gtagaggcac ctgagtgagc aatctgtcgc
    24301 tcgatttttc ggataatact tttcaacctc tggccgcgcg tatgcggcca gaaaatttag
    24361 cacagtatat cggccagcaa catttgctgg ctgcggggaa gccgttgccg cgcgctatcg
    24421 aagccgggca tttacattct atgatcctct gggggccacc gggtaccggc aaaacaaccc
    24481 tcgctgaagt gattgcccgc tatgcgaacg ctgatgtgga acgtatttct gccgtcacct
    24541 ctggcgtgaa agagattcgc gaggcgatcg agcgcgcccg gcaaaaccgc aatgcaggtc
    24601 gccgcactat tctttttgtt gacgaagtgc accgattcaa caaaagccag caggatgcat
    24661 ttctgccaca tattgaagac ggcaccatca cttttattgg cgcaaccact gaaaacccgt
    24721 cgtttgagct taattcggca ctgctttccc gtgcccgtgt ctatctgttg aaatccctgg
    24781 gtacagagga tattgagcaa gtactaactc aggcgatgga agacaagacc cgtggttatg
    24841 gtggtcagga tattgttctg ccagatgaaa cacgacgcgc cattgctgaa ctggtgaatg
    24901 gcgacgcgcg ccgggcgtta aatacgctgg aaatgatggc ggatatggcc gaagtcaatg
    24961 atagcggtaa gcgggtcctg aagcctgaat tattgaccga aatcgccggt gagcgaagcg
    25021 cccgctttga taacaaaggc gatcgctttt acgatctcat ttccgcactg cataagtcgg
    25081 tacgtggtag cgcacccgat gcggcgctct actggtatgc gcgaattatt accgctggtg
    25141 gcgatccgtt atatgtcgcg cgtcgttgcc tggcaattgc ttccgaagat gtcggcaatg
    25201 ccgacccacg ggcgatgcag gtggcaattg cggcctggga ttgctttact cgcgttggcc
    25261 cggcggaagg tgaacgcgcc attgctcagg cgattgttta cctggcctgc gcgccaaaaa
    25321 gcaacgctgt ctacactgcg tttaaagctg cgctggcgga tgctcgcgaa cgtccggatt
    25381 atgacgtgcc ggttcatttg cgtaatgcgc cgacgaaatt aatgaaggaa atgggctacg
    25441 ggcaggaata tcgttacgct catgatgaag caaacgctta tgctgccggt gaggtttact
    25501 tcccgccgga aatagcacaa acacgctatt atttcccgac aaacaggggc cttgaaggca
    25561 agattggcga aaagctcgcc tggctggctg aacaggatca aaatagcccc ataaaacgct
    25621 accgttaatg ttatcgttgc ggtaatgttg ttactgtatc cctgtggtcg caggctgtgg
    25681 ccacatctcc catttaattc gataagcaca ggataagcat gctcgatccc aatctgctgc
    25741 gtaatgagcc agacgcagtc gctgaaaaac tggcacgccg gggctttaag ctggatgtag
    25801 ataagctggg cgctcttgaa gagcgtcgta aagtattgca ggtcaaaacg gaaaacctgc
    25861 aagcggagcg taactcccga tcgaaatcca ttggccaggc gaaagcgcgc ggggaagata
    25921 tcgagccttt acgtctggaa gtgaacaaac tgggcgaaga gctggatgca gcaaaagccg
    25981 agctggatgc tttacaggct gaaattcgcg atatcgcgct gaccatccct aacctgcctg
    26041 cagatgaagt gccggtaggt aaagacgaaa atgacaacgt cgaagtcagc cgctggggca
    26101 ccccgcgtga gtttgacttt gaagttcgtg accatgtgac gctgggtgaa atgcactctg
    26161 gcctcgactt tgcagctgca gttaagctga ctggttcccg ctttgtggta atgaaagggc
    26221 agattgctcg catgcaccgc gcactgtcgc agtttatgct ggatctgcat accgaacagc
    26281 atggctacag tgagaactat gttccgtacc tggttaacca ggacacgctg tacggtacgg
    26341 gtcagctgcc gaaatttgct ggcgatctgt ttcatactcg tccgctggaa gaagaagcag
    26401 acaccagtaa ctatgcgctg atcccaacgg cagaagttcc gctgactaac ctggtgcgcg
    26461 gtgaaatcat cgatgaagat gatctgccaa ttaagatgac cgcccacacc ccatgcttcc
    26521 gttctgaagc cggttcatat ggtcgtgaca cccgtggtct gatccgtatg caccagttcg
    26581 acaaagttga aatggtgcag atcgtgcgcc cggaagactc aatggcggcg ctggaagaga
    26641 tgactggtca tgcagaaaaa gtcctgcagt tgttgggcct gccgtaccgt aaaatcatcc
    26701 tttgcactgg cgacatgggc tttggcgctt gcaaaactta cgacctggaa gtatggatcc
    26761 cggcacagaa cacctaccgt gagatctctt cctgctccaa cgtttgggat ttccaggcgc
    26821 gtcgtatgca ggcacgctgc cgcagcaagt cggacaagaa aacccgtctg gttcataccc
    26881 tgaacggttc tggactggct gttggtcgta cgctggttgc agtaatggaa aactatcagc
    26941 aggctgatgg tcgtattgaa gtaccagaag ttctgcgtcc gtatatgaac ggactggaat
    27001 atattggcta atacccaatt tttctgaatc taaaaagcgc ctgcgggcgc tttttttgtc
    27061 tccctttgat accgaacaat aattactcct cacttacacg taatactact ttcgagtgaa
    27121 aatctaccta tctctttgat tttcaaatta ttcgatgtat acaagcctat atagcgagct
    27181 gctatagaaa taattacaca atacggtttg ttactggaat caatcgtgag caagcttgag
    27241 tgagccatta tgaaaacgaa aatccctgat gcggtattgg ctgctgaggt gagtcgccgt
    27301 ggtttggtaa aaacgacagc gatcggcggc ctggcaatgg ccagcagcgc attaacatta
    27361 ccttttagtc ggattgcgca cgctgtcgat agcgccattc caacaaaatc agacgaaaag
    27421 gttatctgga gcgcctgtac agttaactgt ggtagtcgct gcccgctacg tatgcacgtc
    27481 gtggacggtg aaatcaaata tgtcgaaacg gacaataccg gcgatgacaa ttacgacggc
    27541 ctgcaccagg ttcgcgcctg cctgcgtggg cgttccatgc gtcgccgtgt ctacaatccg
    27601 gaccgcctga aatatccgat gaaacgagtc ggggcgcgcg gtgaaggcaa attcgagcgc
    27661 attagctggg aagaagccta cgacatcatc gcgaccaata tgcagcgcct gatcaaagag
    27721 tacggcaacg agtctatcta tctgaactat ggcaccggta cgctgggcgg caccatgacc
    27781 cgctcctggc cgccgggaaa taccctggtc gcgcggctga tgaactgctg cggcggctat
    27841 ctgaaccatt acggcgacta ctcctccgcg caaattgcgg aaggtttgaa ctatacctac
    27901 ggcggctggg cagatggtaa cagcccgtcg gatatcgaaa acagtaagct ggtagtgctg
    27961 tttggtaata accctggcga aacgcgaatg agtggcggtg gggtgactta ctatcttgaa
    28021 caggcacgcc agaaatctaa tgcccgcatg atcatcattg atccgcgcta taccgacacc
    28081 ggtgccgggc gcgaagatga gtggatccct attcgtccgg gaacagatgc cgcactggtt
    28141 aacggtctgg cgtacgtcat gatcactgaa aacctggtgg atcaggcatt cctcgataaa
    28201 tattgcgttg gctacgatga gaaaaccctg ccagccagtg cgccgaaaaa tggccactat
    28261 aaagcttata ttctgggtga agggccagat ggcgtggcta aaacaccgga atgggcctca
    28321 caaatcaccg gtgttccggc agacaaaatc atcaaactgg ctcgtgaaat cggcagtacc
    28381 aaaccggcgt ttatcagcca gggatggggc ccgcagcgtc acgctaacgg tgaaatcgca
    28441 acccgtgcta tctcgatgct ggcgattctg accggtaacg ttggtattaa cggaggcaac
    28501 agcggcgcgc gtgaaggttc atacagctta ccgtttgtcc gtatgccgac cttggaaaac
    28561 ccgatccaga ccagcatttc gatgtttatg tggaccgatg ccattgaacg tggcccggaa
    28621 atgacggcgc tgcgtgatgg tgtacgcggg aaagataagc tggatgtgcc gatcaaaatg
    28681 atctggaact atgccggtaa ctgcctgatt aaccagcatt ctgaaatcaa ccgtacccat
    28741 gaaatccttc aggatgataa gaagtgcgag ctgattgtgg ttatcgactg ccacatgacc
    28801 tcatcggcga aatatgctga catcctgctg cctgactgca ccgcttccga acagatggac
    28861 tttgcactgg atgcatcctg cgggaatatg tcttacgtga ttttcaacga tcaggtgatt
    28921 aaaccgcgct ttgaatgtaa gaccatctat gaaatgacca gcgaactggc aaaacgtctt
    28981 ggcgttgagc aacagtttac tgaaggccgt acccaggaag agtggatgcg gcatctgtat
    29041 gcccagtcgc gggaagcgat tcctgaactg ccaacgtttg aagagttccg caagcagggg
    29101 atctttaaaa agcgcgaccc acaagggcat cacgttgctt ataaagcctt ccgtgaagat
    29161 ccgcaggcaa acccactgac tacgccatcg ggcaaaattg agatttattc gcaggcgctg
    29221 gctgacattg ccgctacctg ggaattgcca gaaggcgatg tgatcgatcc actgccgatc
    29281 tacacgccgg gctttgaaag ttatcaggat ccgctgaaca aacagtatcc gctgcagctt
    29341 acgggtttcc actataaatc tcgcgttcac tcaacttacg gcaacgttga tgtgctgaaa
    29401 gcggcttgcc gtcaggaaat gtggatcaac ccgcttgatg cgcaaaaacg cggtatcaac
    29461 aacggcgata aagtcaggat ctttaacgat cgtggtgaag ttcatattga ggcgaaagtg
    29521 acgccacgaa tgatgccggg tgtggtcgca ctgggtgaag gtgcctggta tgacccggat
    29581 gcaaaacgtg tcgataaggg cggttgtatt aacgtactga ccactcaacg tccgtctcct
    29641 ctcgctaagg ggaatccgtc acatacaaac cttgttcagg ttgaaaaggt gtaaggagta
    29701 accgatgaca acccagtatg gattttttat tgattccagc cgttgcaccg gttgcaaaac
    29761 ctgcgagctg gcctgtaaag actacaaaga tttgacgcca gaagtcagct tccgccgcat
    29821 ttatgaatat gctggcggcg actggcagga agataacggt gtctggcacc agaacgtgtt
    29881 tgcctactat ctgtcgattt catgtaacca ctgcgaagat ccggcttgta ctaaagtctg
    29941 cccgagcggt gcgatgcata aacgcgaaga tggttttgtt gtggtcgatg aagatgtatg
    30001 cattggctgc cgctactgtc atatggcttg cccgtatggc gcaccgcaat ataacgaaac
    30061 gaaaggccat atgaccaaat gcgatggttg ttatgaccgt gttgcggagg gtaaaaagcc
    30121 gatctgtgtt gaatcttgcc cgctgcgggc gctggatttc ggtcctatcg acgagctgcg
    30181 taaaaaacat ggcgatctgg cggccgttgc gccgttgccg cgagctcact ttaccaaacc
    30241 gaatattgtg atcaaaccca atgccaacag ccgcccgacc ggggatacca ctggctacct
    30301 ggcaaacccg aaggaggtgt gagatgggaa gtggatggca tgaatggccg ctgatgatct
    30361 tcacggtctt cgggcaatgt gtagcgggtg gttttatcgt tctggctttg gcgctgctca
    30421 aaggcgacct gcgagcagaa gcccagcagc gtgttatcgc ctgcatgttt ggcttatggg
    30481 tgctgatggg cattggcttt atcgcctcta tgctccatct tggttcaccg atgcgcgcct
    30541 ttaactcgct caaccgggta ggggcttcag cactcagtaa cgaaatcgcc agcggttcga
    30601 tcttttttgc tgtaggcggc atcggctggt tgctggcaat gctgaaaaag ctgtcaccgg
    30661 cattgcgtac gctatggctg gtggtgacga tggttcttgg cgtcgtcttt gtctggatga
    30721 tggtgcgtgt gtataacagc attgataccg ttccgacctg gtacagcatc tggacgccga
    30781 tgggcttctt cctgacgatg ttcatgggcg gcccgttact gggttacctg ttgctgagtc
    30841 tggctggcgt cgatggctgg gcgatgcgtc tgctgccagc catttctgta ctggcactgg
    30901 tggtgagtgg tgtgatgtca gtgatgcagg gcacagagct ggcaactatt catagttctg
    30961 tgcagcaggc cgcagcgctg gtgccggact atggtgcgct gatgtcctgg cggatcgtgc
    31021 ttttggccgt tgccctgtgc ttgtggattg caccacagct aaaaggttat cagcctgcgg
    31081 ttccgctact atccgtctca ttcattctgc ttctggcagg ggaattaatc ggtcgcggcg
    31141 tattctatgg tttgcatatg accgtgggga tggccgtcgc aagctaatca gtaacaaccg
    31201 gggtttcggc cccggttctc ttttatttct gcttcgttaa cgtgtcataa ctggtcatca
    31261 aattacgata atcaggaata tggttagaga acaacgtcgc cagtccttca atatcattac
    31321 gccagtcacg gtgcagctcg caagccacgc cgaaccatgt catcaaatgc gcacctgctt
    31381 gcgacatgcg atcccatgcc gaatgccggg taatttcatt aaaggtccct gacgcgtcag
    31441 tcacaacaaa gacatcaaac ccttcttcaa tggctgaaag cgccgggaat gccacgcaaa
    31501 cttcggttac cacaccggca ataattaact gttttttacc tgtcgctttg accgctttta
    31561 caaaatcttc gttatcccag gcgttaatat ttcccgggcg agcaatgtaa ggtgcatcgg
    31621 ggaattgtgc tttcagttct ggaactaatg ggccgttagg accggtttca aaactggtgg
    31681 taagaatagt tggtaagttg aaatacttag ccaggtcacc cagcgccagc acgttatttt
    31741 taaacttatc gggttcgata tcccgtacaa gggaaagtaa accagcttgg tgatcaacaa
    31801 gcaaaacggc agcatcattt ttatcaagac gaacatacgg tttggtcatc ctcttctcct
    31861 ttcgagagtc agtggtacgt ctgaaatcgg tcagacgcga tgcattgctc tgaaagcata
    31921 gacgggaaat atgagtttgc tgtgaccatg aaatttttcg actgaacgaa gcgtatgaaa
    31981 tttgtgttag atcaataaaa acaatcagat gagattaatc gtctcttcag cattagcgtt
    32041 atgaatgagt aatataaatc gataataccg ccgctgcgat atgcgcgtat tttcacccat
    32101 tgacaatgtt tttggcggtg gcatgatgcg catgaaattt gaacttcctc acggttttaa
    32161 ttcatgtcca cgtatacccg gcctgtcatg cttttgctgt ctggcctgct tttgttgact
    32221 ctggcgatcg cggtgttaaa tacactcgtg ccgctttggc tcgcccagga acacatgtcc
    32281 acatggcagg taggcgttgt cagctcatcc tattttaccg gcaaccttgt cggtacattg
    32341 ctgacagggt atgtcattaa gcgcattggc tttaaccgca gctattatct ggcctccttc
    32401 atttttgccg ctggctgtgc cggccttggc ctgatgattg gattctggag ctggttggct
    32461 tggcgttttg tcgcgggcgt cggctgtgcc atgatttggg tggttgttga gagcgcgctg
    32521 atgtgcagtg ggacgtcacg taaccgtggg cgtttgcttg ctgcgtatat gatggtttat
    32581 tacgtgggaa cgtttttagg ccagttactg gtcagcaaag tttcaaccga gctgatgtcc
    32641 gtattgccgt gggttacagg tttgacgttg gcagggatct taccgctgtt gtttacgcgt
    32701 gtgctgaatc agcaggctga aaaccatgat tcgacgtcaa ttacgtcaat gctaaaactc
    32761 cgtcaggcgc ggcttggcgt gaatggctgc attatctcag gaatcgttct gggatctcta
    32821 tatggcctga tgccgctgta cctcaatcac aaaggggtga gcaatgccag cattggtttc
    32881 tggatggcgg tactggtcag tgcgggtatc cttggacaat ggccgattgg acgtctggcg
    32941 gataagtttg gtcgactgct ggtgttgcgt gttcaggtct ttgtcgtcat tctcggcagt
    33001 atcgcgatgc ttagccaggc ggcgatggcc ccagcgttat tcatcctcgg tgccgctggc
    33061 tttacgctat atccggtggc gatggcatgg gcttgcgaga aagttgaaca tcatcaactg
    33121 gtggcgatga accaggcctt actgttgagt tatactgtgg gaagtctgct tggcccgtca
    33181 tttaccgcta tgctaatgca gaatttctcc gataatttat tgtttatcat gatcgccagc
    33241 gtatcgttta tctatttgct gatgctgctg cgcaacgccg gtcatacgcc gaaacccgtt
    33301 gctcacgtgt aaatgaattc aagcagagtg tgaacttact gtttcacact ctgctttttt
    33361 gtttcttcta tctgacttgc tttattccaa attttattcg tttaaaaata aaatgtgcag
    33421 caggttataa ttttgcattt cgctatttcc gcacttctta tttgccgcgc ataatccctc
    33481 gttttacaga tgccccttta attttggcga aggatttgtc tatggctggg aatgttcagg
    33541 aaaaacagtt gcgatggtac aacattgcgc tgatgtcttt tatcactgtc tggggttttg
    33601 gcaacgttgt taataactat gccaaccagg ggctggtggt tgttttttca tgggtgttta
    33661 tctttgcact ctatttcaca ccttatgcgc taattgttgg tcagttaggc tcgaccttca
    33721 aagatgggaa gggcggggtc agtacctgga ttaaacacac gatgggaccc ggactggctt
    33781 atctcgccgc gtggacctac tgggtggtgc atattcccta tctggcacaa aaaccccagg
    33841 caattctgat tgcgctcggt tgggcgatga aaggcgacgg ttcgctaatc aaagaatatt
    33901 cagtcgtagc gttacagggg ttaacgctgg tgctgtttat cttctttatg tgggttgctt
    33961 cacgcggtat gaaatcgctg aaaatcgtcg gttctgtggc agggattgcg atgtttgtta
    34021 tgtcgctcct gtatgtggcg atggcggtaa ccgcgcctgc gattactgaa gtgcatattg
    34081 cgaccacaaa cattacctgg gaaacgttca ttcctcatat cgactttacc tacattacca
    34141 ctatttcaat gctggttttc gcggttggcg gagcagagaa gatttctcct tacgttaatc
    34201 aaacgcgcaa cccaggaaaa gaatttccaa aagggatgct atgcctggcg gtgatggttg
    34261 cggtttgtgc cattctgggc tcactggcga tggggatgat gtttgactcg cgtaatatcc
    34321 cggatgactt aatgactaac ggtcaatatt acgcctttca gaagctgggc gagtattaca
    34381 acatgggtaa tactttaatg gtgatttacg ccattgcgaa taccctggga caagtagctg
    34441 cgctggtatt ctcgattgat gccccgctta aagtgctatt aggtgatgct gacagcaaat
    34501 atattccagc cagtttatgt cgtaccaacg cttctggtac gcccgttaat ggctattttc
    34561 tgaccctggt gctggtggca attctgatta tgctgccgac tctcggcatc ggtgatatga
    34621 acaatctcta taaatggctg ttgaatctta actcggtagt gatgccgctg cgttatttat
    34681 gggtatttgt tgcatttatt gcagtcgttc gcctggcgca gaaatataaa ccagaatatg
    34741 tctttattcg taataagcct ctggcaatga ccgtcgggat ttggtgtttt gcctttaccg
    34801 cctttgcctg cttgacgggg atcttcccga aaatggaagc cttcactgca gagtggacct
    34861 tccagttggc gctgaatgtt gcaacgccgt ttgtgctggt aggattagga ctgatattcc
    34921 cgctgctggc gcgtaaagcg aatagtaaat aattattgtg gtcggtgacg ctgactctgg
    34981 cagatctgac ccgtttttac ataatcgaga aatgcgcgca agccgcagga catatactgg
    35041 cggtttggat aatagatctg gaagcctgaa cgctctgtgc tccagtcttc cagtacacat
    35101 tctaaacgac cagtatcaag gtactccttg atctgttcat agagcagata cccgatacca
    35161 gcacccatca aaacggcctc cagttcagca tcaacatcat ccagaatgat atttcctgcg
    35221 acagcaattt ccagctcctt tgcaaattgc cagtggaaag gtttgccgct gggataacga
    35281 aaaacaacac attgatgatt aagcagatca tgaggatggc ggggtttgcc atatcttgca
    35341 aagtactctg gcgttgctgc tacacataat ttcactggtg ggccgatcgc cacgctgatc
    35401 atatcttttt cgacgatgcc actaagtcga acgccagcgt caaagccctg ttggacgata
    35461 tcgacaagag aatcgtcagt tgtgagctcg actttaatat caggatattc acgtgtgaaa
    35521 ccaaccagta aaggcattaa aaatatacgt gcagctaccc gggcggcatt gattttgagc
    35581 gttcctgtcg gtgtcagacg aaaatcattc atctcatcta acatgatctg aatttcgtcg
    35641 aaagctgggc gcaatcgttc atagagatta gaacccgctt ccgttaacga tacgcttcgg
    35701 gttgtccgat tgaataagcg aattttaaga cgttgttcca gtgttttaat actatggcta
    35761 atggcggacg aggataagcc taattcatcg cccgctgcac gaaagctttg attacgggcc
    35821 acggcaaaga aagtggcaaa gtcagacata ttcatccgca ttagtgaaaa tccttcatta
    35881 actcatcctg aatcatcacg tttacaggat tatgccgcct gcgtaaagtg cgctccagaa
    35941 cttaacgtgg aggtaaaatt atgcagtctg aacgtattta tttggtatgg gcccatcctc
    36001 gtcatgattc attgaccgca catattgctg atgcgatcca tcagcgggca atggagcgga
    36061 aaatacaggt gacggaactc gatttatatc ggcgtaattt caacccggtg atgacgtcgg
    36121 aagatgaacc agactggaag aatatggata aacgttattc tccagaggtt catcagcttt
    36181 attcagagct gcttgaacat gacacgttag tggtggtttt tcctttctgg tggtacagct
    36241 tcccggcaat gctaaaagga tatattgaca gagtatggaa taatgggctg gcttatggag
    36301 atgggcacaa attaccattc aataaagttc gttgggtggc gctggttgga ggagacaaag
    36361 aatcatttgt ccagatgggc tgggaaaaaa atataagcga ttatttgaaa aatatgtgca
    36421 gttatcttgg tattgaagat gccgatgtca ctttcttgtg taatacagtg gtattcgatg
    36481 ggcaagaact tcatgcgagc tattatcagt cgttattatc tcaggtacgg gatatggtag
    36541 atgcactata aaatgtgtta aaaacgctgt agcagaatga agcgcggaat aaaaaagcgg
    36601 caactcaata aagttgccgc tttacgggga aattagaaca ttaccttatg accgtactgc
    36661 tcaagaatgc ctttcacgcg ttccatggtc tctttcttag gtggtttaac accgtcgagt
    36721 ttgtactctt cacccattgc cacccatttg tgtttgccca gctcgtggta ggggaggagt
    36781 tcgattttct caacgttgcc catatcacgg gtaaattcac cgaggcgatg cgctgaatcg
    36841 tcatcgtcag accagcctgg gacaacaacg tagcggatcc acaccttcac atttttgttc
    36901 gccagatatt tggcgaactc cagcgtgcgg tggttggaaa ctccaaccag attttggtgg
    36961 atctcgtcgt tcatctgttt gagatcgagc attaccaggt cggttacttc cagcagttca
    37021 tcaatcaccg gatcgtaacg acgaacaaaa ccgttggtgt ccagacaggt atgaatgcct
    37081 tcttttttgc aggcgcggaa ccagtcacga acaaactcag cttgtaggat tgcctcaccg
    37141 ccggatgcgg taacgccgcc gccggaagcg ttcataaagt ggcgataggt caccacctct
    37201 ttcattaaat cttcaacggt aacttcttta ccgccatgcg tatcccaggt gtcgcggtta
    37261 tgacaataca ggcagcgcat caggcagccc tggaaaaagg tgataaagcg aatacctggg
    37321 ccgtctacgg ttccacagga ttcaaaggag tgaatgcgac caataactga cattgcggtg
    37381 tttctccaga tgtggcccat ctgaggccgt gttggtgcgc agctcgaagg ctacgtcgag
    37441 tctgttttgg cagtcacctt aaagtataga tagctgacaa aaaaggctct cgtgctaaaa
    37501 aaggccccac tttcgtggag cctttattgt acgcttttta ctgtacgatt tcagtcaaat
    37561 ctaattacat agattgagtg aaggtacgag taataacgtc ctgctgctgt tctttagtca
    37621 gcgagttgaa acgtactgcg tagccagata cacggatggt cagctgcgga tatttttccg
    37681 ggttttccat cgcgtcgagc agcatttcac ggttcatcac gttaacgttc aggtgctgac
    37741 caccttcgat ggatgcttcg tggtggaagt aaccatccat cagaccagcc aggttggtct
    37801 tacgaacttc gtcgtcttta cccagtgcgt tcggaacgat agagaaggtg taggagatac
    37861 catctttagc gtaagcaaac ggcagtttag caacggaagt cagagacgct acagcacctt
    37921 tctggtcacg accgtgcatc gggttagcac ccggtccgaa cggcgcgcca gcacgacgac
    37981 cgtctggggt gttaccagtt ttcttaccat acacaacgtt agaagtgatg gtcagaacag
    38041 actgagtcgg gatagcgtca cggtaggtgt gcagtttctg aattttcttc atgaaacgtt
    38101 ctaccaggtc aacagccagg tcatctacac gcggatcatt gttaccaaac tgcgggtatt
    38161 cgccttcgat ttcgaagtcg atagccagac cgtcttcgtc acgaatcggt ttaactttcg
    38221 catatttgat tgcagacagg gagtcagcag caacggacag accagcgata ccacacgcca
    38281 tggtgcggat aacgtcacgg tcgtgcagcg ccatcagaga ggcttcgtag ctgtacttgt
    38341 cgtgcatgta gtggatgatg ttcagtgcag tgatgtactg tttagccagc cagtccatga
    38401 agtgatccat gcgctccatc acttcatcat agttcaggac atcgcctttg atcggttcag
    38461 acttcggacc aacctgcatt ttcagttttt cgtcaacgcc gccgttgatt gcgtacagca
    38521 tggttttcgc caggtttgca cgcgcaccga agaactgcat ttgtttacca acgatcatcg
    38581 ggcttacgca gcaagcaata gcgtagtcat cgttgttgaa gtccggacgc atcaggtcat
    38641 cgttctcata ctgcagagaa gaggtgtcga tggacacttt agcggcgaat ttcttgaagt
    38701 tcagcggcag tttttcagac cacagaatgg tcatgttcgg ttccggagac ggacccatgg
    38761 tgtacagggt gttcaggaaa cggaagctgt ttttggtaac cagggtacga ccgtcgaggc
    38821 ccataccacc gatagattcg gttgcccaga tcgggtcgcc agagaacagt tcatcgtatt
    38881 ccggagtacg caggaagcga accatacgca gtttcatgac caggtggtca accatttcct
    38941 gcgcttcttg ttcggtgatc ttgccagctt tcaggtcacg ttcgatgtac acatccagga
    39001 aggtggaggt acgaccgaag gacattgcag caccgttctg agacttaaca gcagccaggt
    39061 agccgaagta agtccactgg atagcttcct gagcgttggt agccggacca gagatgtcgt
    39121 agccgtattt cgcagccatt tctttcatct gacccagagc gcggtgctgt tcagcgattt
    39181 cttcgcgcag acggatagtc tgttccaggt ttacgccgtt ttccagatca gcctgcagag
    39241 aagtgaactg tgccagtttg tctttcatca ggtagtcgat accgtacagc gcaacgcgac
    39301 ggtagtcacc gatgatacgg ccacggccat atgcatctgg cagaccggtc agaacaccag
    39361 atttacggca acgcaggatg tccggagtgt aaacgtcgaa cacgccctgg ttgtgagttt
    39421 tacggtattc agtgaagatt tttttgatca tcggatccag ttcgcggttg tacgctttgc
    39481 aggaaccttc gatcattttg ataccaccga acgggataag agcacgtttc agcggagctt
    39541 cagtctgcag accaacgatt ttctcaagct gcttgttgat gtagccagcg tcgtgagagg
    39601 tgatggtgga agcaacagcg gtgtcaaagt caactggcgc gtgagtgcgg ttttccagtt
    39661 taacgccttc cattactttg tcccacaggg tggtggtcgc ttcagtagcg ccagccagga
    39721 aggactcgtc accctcgtac ggagtgtagt ttttctgaat gaagtcacgg acgtttactt
    39781 cattctgcca gtcacctttg gtaaaacctt cccaggctgt ggctaacttt tcattaagct
    39841 cggacatgta acacctacct tcttaagtgg attttttatt tactgcgtac ttcgacaacc
    39901 attaatggtg atcgttttca cgcaggtaaa tgacccagta tgtcaaccca accaacaaac
    39961 caccgccgat aatattaccg atcgtaaccg gaatcaggtt atcagtgatg aagttcatca
    40021 cggtcaggtg agaaaaattt tccggtgcag aaccgacagc ggtccagaat tccggagatg
    40081 cgaagtcgcg gattacaata cccatcggga tcataaacat gtttgcgata ctgtgctcaa
    40141 aaccgctggc aacaaacatc gcgaccggca gcaccataat gaacgctttg tccatcaggc
    40201 tacggccaga ataactcatc catactgcca gacataccat caggtttgcc aggataccaa
    40261 ggcagacggc ctcaataaaa gtatggtgca ctttgtggtc ggcggtttgt aggacgttta
    40321 gtccccattg accatttgcg gtcatatact cgccggaaag ccacattaaa agtacaaaca
    40381 gcagtgcgcc gaccaggttg ccaaaataga catttagcca gtttttcgcc aactgacccc
    40441 aggtgatgcg cccactcgcc ttagcaacaa caatcaacac tgtggaagta aagagatcgg
    40501 ctccgcagac aacacaaaga atcagcccca gagagaagca aatgccgcca accagttttg
    40561 ccatgccgaa gggcattgtg cctgtgccag tggttgctgt gatatagaag acgaatgcga
    40621 ttgagatgaa aacaccggcg gtaatcgcca gatagaaagt cttaagcgga tgtttcgttg
    40681 ctttatagac acccgcctct tcggccactt tggccattgc agcaggaagt aaaagatcaa
    40741 aagggttgtc agctttcaca ctaactctct ctttattaag tcggcgacga gatactaaca
    40801 aagcattata gatgagaaat tgatatagat catatctcgc ctggcttata ggcccgtaac
    40861 tcgcatggtt tttatgcaaa tacggagtaa atatttgatt atccaaataa aaataaattt
    40921 taaaaattaa caaatgagtt gaattttttc cgcatcctcc gctaaaacag ttaattaaaa
    40981 gggagcatca
//
LOCUS       DAZGAN020000034        39792 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGAN020000034 DAZGAN020000000
VERSION     DAZGAN020000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.034"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..206)
                     /locus_tag="ACHKYO_RS09970"
                     /old_locus_tag="pgaptmp_001994"
                     /pseudo
     CDS             complement(1..206)
                     /locus_tag="ACHKYO_RS09970"
                     /old_locus_tag="pgaptmp_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(435..1355)
                     /gene="malM"
                     /locus_tag="ACHKYO_RS09975"
                     /old_locus_tag="pgaptmp_001995"
     CDS             complement(435..1355)
                     /gene="malM"
                     /locus_tag="ACHKYO_RS09975"
                     /old_locus_tag="pgaptmp_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HGU4428850.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1598..2938)
                     /gene="lamB"
                     /locus_tag="ACHKYO_RS09980"
                     /old_locus_tag="pgaptmp_001996"
     CDS             complement(1598..2938)
                     /gene="lamB"
                     /locus_tag="ACHKYO_RS09980"
                     /old_locus_tag="pgaptmp_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HGU4428851.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3010..4125)
                     /gene="malK"
                     /locus_tag="ACHKYO_RS09985"
                     /old_locus_tag="pgaptmp_001997"
     CDS             complement(3010..4125)
                     /gene="malK"
                     /locus_tag="ACHKYO_RS09985"
                     /old_locus_tag="pgaptmp_001997"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HGU4428852.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4490..5680
                     /gene="malE"
                     /locus_tag="ACHKYO_RS09990"
                     /old_locus_tag="pgaptmp_001998"
     CDS             4490..5680
                     /gene="malE"
                     /locus_tag="ACHKYO_RS09990"
                     /old_locus_tag="pgaptmp_001998"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HGU4428853.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            5834..7378
                     /gene="malF"
                     /locus_tag="ACHKYO_RS09995"
                     /old_locus_tag="pgaptmp_001999"
     CDS             5834..7378
                     /gene="malF"
                     /locus_tag="ACHKYO_RS09995"
                     /old_locus_tag="pgaptmp_001999"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HGU4428854.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGNGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            7393..8283
                     /gene="malG"
                     /locus_tag="ACHKYO_RS10000"
                     /old_locus_tag="pgaptmp_002000"
     CDS             7393..8283
                     /gene="malG"
                     /locus_tag="ACHKYO_RS10000"
                     /old_locus_tag="pgaptmp_002000"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HGU4428855.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8655..10130
                     /gene="xylE"
                     /locus_tag="ACHKYO_RS10005"
                     /old_locus_tag="pgaptmp_002001"
     CDS             8655..10130
                     /gene="xylE"
                     /locus_tag="ACHKYO_RS10005"
                     /old_locus_tag="pgaptmp_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HGU4428856.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10174..10584)
                     /gene="psiE"
                     /locus_tag="ACHKYO_RS10010"
                     /old_locus_tag="pgaptmp_002002"
     CDS             complement(10174..10584)
                     /gene="psiE"
                     /locus_tag="ACHKYO_RS10010"
                     /old_locus_tag="pgaptmp_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HGU4428857.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            10799..11077
                     /locus_tag="ACHKYO_RS10015"
                     /old_locus_tag="pgaptmp_002003"
     CDS             10799..11077
                     /locus_tag="ACHKYO_RS10015"
                     /old_locus_tag="pgaptmp_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4428858.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11124..13220)
                     /locus_tag="ACHKYO_RS10020"
                     /old_locus_tag="pgaptmp_002004"
     CDS             complement(11124..13220)
                     /locus_tag="ACHKYO_RS10020"
                     /old_locus_tag="pgaptmp_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU4428859.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13220..13957)
                     /locus_tag="ACHKYO_RS10025"
                     /old_locus_tag="pgaptmp_002005"
     CDS             complement(13220..13957)
                     /locus_tag="ACHKYO_RS10025"
                     /old_locus_tag="pgaptmp_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU4428860.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(13954..14592)
                     /locus_tag="ACHKYO_RS10030"
                     /old_locus_tag="pgaptmp_002006"
     CDS             complement(13954..14592)
                     /locus_tag="ACHKYO_RS10030"
                     /old_locus_tag="pgaptmp_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HGU4428861.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNIPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVIQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(14706..14948)
                     /gene="yjbE"
                     /locus_tag="ACHKYO_RS10035"
                     /old_locus_tag="pgaptmp_002007"
     CDS             complement(14706..14948)
                     /gene="yjbE"
                     /locus_tag="ACHKYO_RS10035"
                     /old_locus_tag="pgaptmp_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HGU4428862.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15303..16952)
                     /gene="pgi"
                     /locus_tag="ACHKYO_RS10040"
                     /old_locus_tag="pgaptmp_002008"
     CDS             complement(15303..16952)
                     /gene="pgi"
                     /locus_tag="ACHKYO_RS10040"
                     /old_locus_tag="pgaptmp_002008"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGU4428863.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRD"
     regulatory      17189..17385
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17477..18826
                     /gene="lysC"
                     /locus_tag="ACHKYO_RS10045"
                     /old_locus_tag="pgaptmp_002009"
     CDS             17477..18826
                     /gene="lysC"
                     /locus_tag="ACHKYO_RS10045"
                     /old_locus_tag="pgaptmp_002009"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HGU4428864.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            18971..19897
                     /locus_tag="ACHKYO_RS10050"
                     /old_locus_tag="pgaptmp_002010"
     CDS             18971..19897
                     /locus_tag="ACHKYO_RS10050"
                     /old_locus_tag="pgaptmp_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HGU4428865.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYNKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(19933..20724)
                     /locus_tag="ACHKYO_RS10055"
                     /old_locus_tag="pgaptmp_002011"
     CDS             complement(19933..20724)
                     /locus_tag="ACHKYO_RS10055"
                     /old_locus_tag="pgaptmp_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HGU4428866.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVIYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(20730..21857)
                     /locus_tag="ACHKYO_RS10060"
                     /old_locus_tag="pgaptmp_002012"
     CDS             complement(20730..21857)
                     /locus_tag="ACHKYO_RS10060"
                     /old_locus_tag="pgaptmp_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HGU4428867.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAELVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            22387..22659
                     /locus_tag="ACHKYO_RS10065"
                     /old_locus_tag="pgaptmp_002013"
     CDS             22387..22659
                     /locus_tag="ACHKYO_RS10065"
                     /old_locus_tag="pgaptmp_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HGU4428868.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(22660..23532)
                     /gene="rluF"
                     /locus_tag="ACHKYO_RS10070"
                     /old_locus_tag="pgaptmp_002014"
     CDS             complement(22660..23532)
                     /gene="rluF"
                     /locus_tag="ACHKYO_RS10070"
                     /old_locus_tag="pgaptmp_002014"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HGU4428869.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            23744..24433
                     /gene="pepE"
                     /locus_tag="ACHKYO_RS10075"
                     /old_locus_tag="pgaptmp_002015"
     CDS             23744..24433
                     /gene="pepE"
                     /locus_tag="ACHKYO_RS10075"
                     /old_locus_tag="pgaptmp_002015"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HGU4428870.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(24524..26155)
                     /locus_tag="ACHKYO_RS10080"
                     /old_locus_tag="pgaptmp_002016"
     CDS             complement(24524..26155)
                     /locus_tag="ACHKYO_RS10080"
                     /old_locus_tag="pgaptmp_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGU4428871.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(26375..30058)
                     /gene="metH"
                     /locus_tag="ACHKYO_RS10085"
                     /old_locus_tag="pgaptmp_002017"
     CDS             complement(26375..30058)
                     /gene="metH"
                     /locus_tag="ACHKYO_RS10085"
                     /old_locus_tag="pgaptmp_002017"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HGU4428872.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            30258..31082
                     /gene="iclR"
                     /locus_tag="ACHKYO_RS10090"
                     /old_locus_tag="pgaptmp_002018"
     CDS             30258..31082
                     /gene="iclR"
                     /locus_tag="ACHKYO_RS10090"
                     /old_locus_tag="pgaptmp_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HGU4428873.1"
                     /translation="MVAPIPAKRGRKPAVATAPAIGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            31399..32972
                     /locus_tag="ACHKYO_RS10095"
                     /old_locus_tag="pgaptmp_002019"
                     /pseudo
     CDS             31399..32972
                     /locus_tag="ACHKYO_RS10095"
                     /old_locus_tag="pgaptmp_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(32941..34677)
                     /gene="aceK"
                     /locus_tag="ACHKYO_RS10100"
                     /old_locus_tag="pgaptmp_002020"
     CDS             complement(32941..34677)
                     /gene="aceK"
                     /locus_tag="ACHKYO_RS10100"
                     /old_locus_tag="pgaptmp_002020"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HGU4428874.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(34958..36262)
                     /gene="aceA"
                     /locus_tag="ACHKYO_RS10105"
                     /old_locus_tag="pgaptmp_002021"
     CDS             complement(34958..36262)
                     /gene="aceA"
                     /locus_tag="ACHKYO_RS10105"
                     /old_locus_tag="pgaptmp_002021"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HGU4428875.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(36292..37893)
                     /gene="aceB"
                     /locus_tag="ACHKYO_RS10110"
                     /old_locus_tag="pgaptmp_002022"
     CDS             complement(36292..37893)
                     /gene="aceB"
                     /locus_tag="ACHKYO_RS10110"
                     /old_locus_tag="pgaptmp_002022"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HGU4428876.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISEMASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGIAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(38162..39091)
                     /gene="metA"
                     /locus_tag="ACHKYO_RS10115"
                     /old_locus_tag="pgaptmp_002023"
     CDS             complement(38162..39091)
                     /gene="metA"
                     /locus_tag="ACHKYO_RS10115"
                     /old_locus_tag="pgaptmp_002023"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGU4428877.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            39248..39691
                     /locus_tag="ACHKYO_RS10120"
                     /old_locus_tag="pgaptmp_002024"
     CDS             39248..39691
                     /locus_tag="ACHKYO_RS10120"
                     /old_locus_tag="pgaptmp_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU4428878.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         9646 a        10334 c         9947 g         9865 t
ORIGIN      
        1 acgaaacaaa tttattgcat gattttttcg ttccgttgtt ttatatttga aaaaatattg
       61 ctgatataaa taaagttatc tctgtttaaa cctggcaccc cattgaaatt acgtagtaaa
      121 tcaagatcta ctatattatt tccttgcacc acatcatgtt tcatcaaaga gttattgccg
      181 ccagaatttg ggatatgact tatcataaaa cctctgcata acagaaataa aataacatgg
      241 aaaagtagcc tgaatgtcca gacactaccg gcgaaaggaa aagaatctct gataaggcat
      301 tgagataatg gatattctta gtgaagaaga agtgagttac tataaaaaat cgctcatatc
      361 ccaggaagga agcataaaaa aaggtgcgcc aggagacgca ccagttgttg caaaatcagc
      421 actgtggggc gtaattaccc cttgcctttt acactgctga taaaggtgga acgggcagat
      481 gtcgatccca ggcgttcagc ttcatcaagc agttttaacg ccttatcaac atcacctttc
      541 gcgacagcgt ttttgatcgc ggtattaaaa taactttccg tgtcgttgag catcggctcg
      601 cttttcttca ccggtgccgg agcgggtgca gccacagctg gtgccgccgt gttacctacc
      661 gtaaccggag ctggagcgga agaaccaaat aaaggtccta ccaacacgct ggagctggag
      721 ttcgttttca ctttcagttt cagtaagcca tcggtggtat gacgagcaac cggatcgggg
      781 atatccggga tcgagttacc gacgcccttg gcataggctt tagccgggtc gagcagttgg
      841 gtcgtctgct ggagatcttt ttccgtggta aagaccagaa cataaagttt ttgctgcccc
      901 aacgccggtg tcaggcgcat aacgccttcc agccgatctg cactcatcac gcctggttcc
      961 tggtaggtga aataactgct ggggaagaag gctgatgggg tcatgttctg atcaagaatc
     1021 agcacgttcg gcgcaaaaac gctggtttgt ttgttcactt cgctggtcag cgtcagggtc
     1081 agttcgccaa tgtttgccgg gacgctgtac gcagcaaccg gaccactgat gccgggaacg
     1141 ttcagttgtt ggccgccggt cgccagttgg gtggtctggg ttttagattg atcgaccggt
     1201 gtccaggtga gttgttgcag cgcggcagat ggaatggctg gcgcgtcgct ggtgttttgc
     1261 ggtacgtagt taacatcggc aaggctgatt ccaggcgcgc ttgccagtaa ccctgctgat
     1321 aaacagaggg cgatgagact tttattcatt ttcattgttt tcacctcaaa atctggagct
     1381 cagcggtagc caggcaatag cgcgctaaac ccgataatca gaggggcttt cgcccctttc
     1441 agataatgac aacctgtttt tatgccggat gcggcgtaaa cgccttatcc ggcctacatt
     1501 tgacagccgt tgtaggcctg ataagacgcg caagcgtcgc atcaggcgtt ggttgccgaa
     1561 tgcggcgtaa acgccttatc cggcccaggt tttgctatta ccaccagatt tccatctggg
     1621 caccgaaggt ccactcgtcg ctgtcgccac gaccgaagct gccgccgttg aaatcagcag
     1681 gaacggcttt gccgtagttc gggttaacct tgctatcgcc gttgtagtca taaccccatt
     1741 tctcatccca cttggcgtag gttgcgaaga cacgaatagc cgggcgtgac cagatgctgt
     1801 cgccagcctg ccattgttgt gcgagggtaa ttttgtactg attgttcttg tcgccggtgc
     1861 gctgggattc gacgttgtcg tagccgattt ccatcacggt gctcatgatt ggcgtccact
     1921 tgtacatcgg gcgaatacca acggtccacc acttggtgcc gttgtcgtta tcccagttga
     1981 tatcctggta catacccacg tacatcatgt cccagttgtc gcccatggag atcgcaccgt
     2041 ggtcgaggat acgcagcatg tgaccgttgt tgttgatatt gtaggcaaat ttttcgttat
     2101 caaacgcgac gccagaaccc tgcgacagac ctttaccctg cgaggtcatc gagtcagtag
     2161 cgtactgaac aacaaacttg ttaaagccct tcaggacact ctgagtatgt tcagcagtga
     2221 ataaccagcc gtctttcgat gcgccatcaa ccagacgata gttatcacgc aggttggcac
     2281 gaccgtagtc gacacccagt tctaatgtgc cgcccgggtt gatttccatc tgcgctaaac
     2341 gcacatcgaa aacgtcgttc gcggtttcgt tggtatagtc ataaatattg ttgctggcaa
     2401 aagaggaaga accaccagct tcagaggagc gggttgctgc cagagagagt ttaccgaagc
     2461 caacatcgat gttttccaga ccggcaccag gaccagaaat atcccagtag tagaagtcga
     2521 tcatatgaac gtcatgacgt tggtagaagc gcttacctgc ccagatggtg gaacctggca
     2581 gccattcgat caggttttta ccctgcacgt ttgcttcacg gaaggccgga tcggtagctt
     2641 cccagtcatt ctgttgcgcg acggaatagg ccacgttagt gtcgaaatag aagctcttat
     2701 cgccctcttt ccacacttcc tgacccaatt ttaattcagc ataagtttca cattcgttgc
     2761 caagacggta tttactttga gcaccggtag tctggaaaca ctgttgttca ccgccgctac
     2821 ctgtccagcc aataccggaa cgtgcatagc cgtggaaatc aacagccatt gcctgagcag
     2881 acattacgcc cgctgcgacg gcaaccgcca gaggaagttt gcgcagagta atcatcattc
     2941 tatctcctga gtcattgctt ttcttttttc acatcacctg tgacaggctt tgtgtgtttt
     3001 gtggggtgct taaacgcccg gctccttatg cagtcgacga catgcagtgc catcctcacg
     3061 gaacagatgg caacgctctg gcggcaggcc gatagcgaat gtggcacctt cttctaccaa
     3121 caccacgtcg ttctggcggt acaccaggtt ttgacgaatg gaagggatct ggatatggat
     3181 ttgggtttcg ttgccgagtt gctcgacgac ctgaacttca ccctcaagga tgacgtcagc
     3241 gatatcactc ggcagtagat gttccgggcg aatacccagc gacatattgg ctccaacctg
     3301 gacatcacgg ctttcaactg gcagccagac ttgctgacga tttggcatcg gcagctccac
     3361 ctgcacttga tcgattgcgg tggcggtcac ttttaccggc aggaagttca tctttggcga
     3421 accgataaat ccggcgacaa aacggtctgc cggatagtgg tacagctcca gcggtttccc
     3481 aacctgcgcc acgcgaccgg cgtccagcac cacgattttg tcggccagcg tcatcgcttc
     3541 gacctgatcg tgggtgacgt aaatcattgt gcggcccagg cgtttatgca gacgggagat
     3601 ttcgatacgc atttgcacac gcagtgcagc atcgaggttg gagagcggtt catcgagcaa
     3661 aaatacgctt ggctcggcca ccagcgtacg gccaatcgcc acacgctgac gctgaccgcc
     3721 agagagcgct ttcggtttgc gatccagcaa atgcgccagt tgcagcactt ccgccacctg
     3781 gttaacgcgt tggttaatca cctctttttt tgcgcctgcc agtttcaggc caaatgacat
     3841 attttctgct actgacaggt ggggatagag cgcgtaagac tgaaacacca taccaacgcc
     3901 gcgttctgct ggcggagtgt cattcatccg tttctcaccg atgaacaggt cgccgctggt
     3961 gatcgtctca agcccggcaa tcatgcgcag taaagtcgat ttaccgcagc cagacggtcc
     4021 gacaaacacc acgaattcac cttcatggat atcgagattg atatctttcg ataccacgac
     4081 ctcgccccag gctttcgtta cattttgcag ctgtacgctc gccatgccct tctccctttg
     4141 taacaacctg tcatcgacag caacattcat gatgggctga ctatgcgtca tcaggagatg
     4201 gcttaaatcc tccaccccct ggctttttta tgggggagga ggcgggagga tgagaacacg
     4261 gcttctgtga actaaaccga ggtcatgtaa ggaatttcgt gatgttgctt gcaaaaatcg
     4321 tggcgatttt atgtgcgcat ctccacatta ccgccaattc tgtaacagag atcacacaaa
     4381 gcaacggtgg ggcgtagggg caaggaggat ggaaagaggt tgccgtataa agaaactaga
     4441 gtccgtttag gtgttttcac gagcacttca ccaacaagga ccatagatta tgaaaataaa
     4501 aacaggtgca cgcatcctcg cattatccgc attaacgacg atgatgtttt ccgcctcggc
     4561 tctcgccaaa atcgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa
     4621 cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga
     4681 gcatccggat aaactggaag agaaattccc tcaggttgcg gcaactggcg atggccctga
     4741 cattatcttc tgggcacacg accgctttgg tggctacgct caatctggcc tgttggctga
     4801 aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacctggg atgccgtacg
     4861 ttacaacggc aagctgattg cttacccgat cgctgttgaa gcgttatcgc tgatttataa
     4921 caaagatctg ctgccgaacc caccaaaaac ctgggaagag atcccggcgc tggataaaga
     4981 actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg
     5041 gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca agtacgatat
     5101 taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct
     5161 tattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa
     5221 taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag
     5281 caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat ctaaaccgtt
     5341 cgttggcgtg ctgagcgccg gtattaacgc cgccagtccg aacaaagagc tggcgaaaga
     5401 gttcctcgaa aactatctgc tgactgacga aggtctggaa gcggtcaata aagacaaacc
     5461 gctgggtgca gtggcgctga agtcttacga ggaagagttg gcgaaagatc cacgtattgc
     5521 cgccaccatg gaaaacgccc agaaaggtga aatcatgccg aacatcccgc agatgtccgc
     5581 tttctggtat gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc agactgtcga
     5641 tgaagccctg aaagacgcgc agactcgtat caccaagtaa tgctatgaaa tgccggatgc
     5701 ggcgtgaacg ccttgtccgg cctacaaaac tgaaacgcat gtaggcctga taagacgcat
     5761 cagtgtcgta tcaggcagtt gttgtcggat aaggcgtgaa agccttatcc gtcctggaat
     5821 gaggaagaac cccatggatg tcattaaaaa gaaacattgg tggcaaagcg acgcgctgaa
     5881 atggtcagtg ctaggtctgc tcggcctgct ggtgggttac cttgttgttt taatgtacgc
     5941 acaaggggaa tacctgttcg ccattaccac gctgatattg agttcagcgg ggctgtatat
     6001 tttcgccaat cgtaaagcct acgcctggcg ctatgtttac ccgggaatgg ctggaatggg
     6061 attattcgtc ctcttccctc tggtctgcac catcgccatt gccttcacca actacagcag
     6121 cactaaccag ctgacttttg aacgtgcgca ggaagtgttg ttagatcgct cctggcaagc
     6181 aggcaaaacc tataactttg gtctttaccc ggcgggcgat gagtggcaac tggcgctcag
     6241 cgacggcgaa accggtaaaa attacctctc cgacgcgttt aaatttggcg gcgagcaaaa
     6301 actgcaactg aaagaaacga ccgcccagcc cgaaggcgaa cgcgcgaatc tgcgcgtgat
     6361 tacccagaac cgccaggcgc tgagtgacat taccgccatt ctgccggatg gcaacaaagt
     6421 gatgatgagc tccctgcgcc agttttctgg cacgcagccg ctctacacac tcgacggtaa
     6481 cggcacgttg acgaataatc agagtggcgt gaaatatcgt ccgaataacc aaattggctt
     6541 ttaccagtcc attaccgccg acggcaactg gggggatgaa aagctaagcc ccggttacac
     6601 cgtgaccacc ggctggaaaa actttacccg cgtctttacc gacgaaggca ttcagaaacc
     6661 gttcctcgcc attttcgtct ggaccgtggt gttttcactg atcaccgtct ttttaacggt
     6721 ggcggtcggc atggttctgg cgtgtctggt gcagtgggaa gcgttgcgtg gcaaagcggt
     6781 atatcgcgtc ctgctgattc tgccctacgc ggtgccatca ttcatttcaa tcttgatttt
     6841 caaagggttg tttaaccaga gcttcggtga aatcaacatg atgttgagcg cgctgtttgg
     6901 cgtgaagccc gcctggttca gcgatccgac cactgcccga acgatgctga ttatcgtcaa
     6961 tacctggctg ggttatccgt acatgatgat cctctgcatg ggcttgctga aagcgattcc
     7021 ggacgatttg tatgaagcct cagcaatgga tggcgcaggt ccgttccaga acttctttaa
     7081 gattacgctg ccgctgctga ttaaaccgct gacgccgctg atgatcgcca gcttcgcctt
     7141 taactttaac aacttcgtgc tgattcagct gttaaccaac ggcggcccgg atcgtcttgg
     7201 cacgaccacg ccagccggtt ataccgacct gcttgttaac tacacctacc gtatcgcttt
     7261 tgaaggcggc gggggtcagg acttcggtct ggctgcagca attgccacgc tgatcttcct
     7321 gctggtgggt gcgctggcga tagtgaacct gaaagccacg cgaatgaagt ttgattaagg
     7381 gagataacaa aaatggcaat ggtccaaccg aaatcgcaaa aagcacgttt gtttattact
     7441 cacctgctac tgctactttt tatcgcggcg attatgttcc cgctgctgat ggtcgtcgct
     7501 atctcgctgc gtcagggaaa cttcgcgacc ggtagcctga tcccggagca aatctcctgg
     7561 gatcactgga aactggcgtt aggttttagc gttgaacagg ctgatggtcg cattacgcca
     7621 ccgccattcc cggtactgct gtggctgtgg aactcggtaa aggtcgccgg gatttccgcg
     7681 attggcattg tggcgctctc caccacctgc gcctacgctt tcgcccgtat gcgctttcca
     7741 ggcaaagcga cgctgctgaa aggaatgctg attttccaga tgttcccggc agtactttca
     7801 ctggtcgcgt tgtatgcgtt gtttgatcgt ctgggtgagt acattccatt cattggcctg
     7861 aatactcacg gcggcgtaat tttcgcgtat ctgggtggga ttgcgctgca tgtctggacc
     7921 atcaaaggct atttcgaaac catcgacagt tcgctggaag aagcggctgc gctggatggt
     7981 gcgacaccgt ggcaggcctt ccgccttgtc ctgttgccgc tgtcagtacc gattctggcg
     8041 gtggtattca tcctgtcgtt tatcgctgcc attactgaag ttccggtcgc gtcgctgtta
     8101 ctgcgtgacg taaacagcta caccctggcc gtggggatgc agcaatacct caacccgcaa
     8161 aactacctgt ggggtgactt tgccgccgct gccgtgatgt ctgcattacc gatcaccatc
     8221 gtcttcttgc tggctcaacg ctggctggtc aacggcctga cggcaggtgg tgtgaaaggt
     8281 taaagatgtt gttctgccaa tgttatgccg ctgcaccctc aacttacgtt atcccaactt
     8341 gtgactgtta ttcggcgctc cacggagcgc ctttttttct ttcgtctgca atctgaatcg
     8401 ttcgccggtt aatattccca tcatagagct tattattttt acgttatttg ttttcccact
     8461 tacgataatt ctctttcgtg ctctgagtca cggcaatagt attgttttca tcaatttttg
     8521 ataattatca caattaagat cacagaaaag acattacgta aacgcattgt aaaaaatgat
     8581 aattgcctta actgcctgac aattccaaca tcaatgcact gataaaagat cagaatggtc
     8641 taaggcaggt ctgaatgaat acccagtata attccagtta tatattttcg attaccttag
     8701 tcgctacatt aggtggttta ttatttggct acgacaccgc cgttatttcc ggtactgttg
     8761 agtcactcaa taccgtcttt gttgctccac aaaacttaag tgaatccgct gccaactccc
     8821 tgttaggatt ttgcgtagcc agcgctctga ttggttgcat catcggcggt gccctcggtg
     8881 gttattgcag taaccgcttc ggccgtcgtg attcacttaa gattgctgct gtcctgtttt
     8941 ttatttctgg tgtaggttct gcctggccag aacttggttt tacctctata aacccggaca
     9001 acacagtccc tatttatctg gcaggttatg tcccggaatt tgttatttat cgtattattg
     9061 gcggtattgg cgttggttta gcctcaatgc tttcaccaat gtatattgcg gaactggctc
     9121 cagctcatat tcgcggaaaa ctggtttcat ttaaccagtt tgcgattatt ttcgggcaac
     9181 ttttagttta ctgcgtaaac tattttattg cccgttccgg tgatgccagc tggctgaata
     9241 ctgacggctg gcgttatatg tttgcctcgg aatgtatccc tgcactgctg ttcttaatgc
     9301 tgctgtatac cgtgccagaa agtcctcgct ggctgatgtc acgcggcaag caagaacagg
     9361 cggaaagtat cctgcgcaaa attatgggca acacgcttgc aactcaggca gtacaggaaa
     9421 ttaaacactc cctggatcat ggccgcaaaa ccggtggtcg tctgctgatg tttggcgtgg
     9481 gcgtgattgt aatcggcgtt atgctctcca tcttccagca atttgtcggc atcaatgtgg
     9541 tgttgtacta cgcgccggaa gtgttcaaaa cgctgggggc cagcacggat atcgcgctgt
     9601 tgcagaccat tattgtcgga gttatcaacc tcaccttcac cgttctggca attatgacgg
     9661 tggataaatt tggtcgtaag ccactgcaaa ttatcggcgc gctcggaatg gcaatcggta
     9721 tgtttagcct cggtaccgcg ttttacactc aggcaccggg tattgtggcg ctactgtcga
     9781 tgctgttcta tgttgccgcc tttgccatgt cctggggtcc ggtatgctgg gttctgctgt
     9841 cggaaatctt cccgaatgcc attcgtggta aagcgctggc aatcgcggtg gcggcccagt
     9901 ggctggcgaa ctacttcgtc tcctggacct tcccgatgat ggacaaaaac tcctggctgg
     9961 tggcccattt ccacaacggt ttctcctact ggatttacgg gtgtatgggc gttctggcag
    10021 cactatttat gtggaaattt gtcccggaaa ccaaaggtaa aacccttgag gagctggaag
    10081 ccctctggga accggaaacg aagaaaacac aacaaactgc tacgctgtaa tcttcctgtc
    10141 cagcacgccg cgccagttcg gcgtgctgtc tttttactcc cgcttcagcc gtttcgaatt
    10201 acacaaccac agagtgatca ccagtaacag gatcgcagcc gagtagatca acacatccag
    10261 tggcgattta tgatcgacga tgatcaagcg cacaatggcg gtgatcccaa tatagacaaa
    10321 gtaacgtagc gggaagtgaa aaccggactg aaagtatttc acaatcagcg cgataaattc
    10381 gaaatagaga aagtaaacca ccagtccttc taccagctca tatttgctgg tttgttctgg
    10441 cgcgaacagc acatctgcca gatgcaccgt ttctttgccg aggaagacaa ccaaaatcag
    10501 gccgaggcac aacaggccaa gattgagtac ggtctgcaaa atggtggaga taaactccac
    10561 gcgcggacga gagagtgacg tcatagcagt acctccttca cagtggcgag cttcctgtat
    10621 aacgcaaaaa tgtgacggag atctatattt tttgtgtatg ttttgttcag cttgattaag
    10681 tgggcatctc ccatcaacgt cgcatctggc aaaacacaca gccccaaaaa atcacccatt
    10741 caggtgattc ccagccacca gcaatgcgcg aaaaatcgac catgttcctc tggaacaaat
    10801 gaaacgtaac ctgataaagg tggtaaaaat gaagccttat tttgctgctt tgatgttatc
    10861 agtctctgtt cttcctgctt atgctggtcc gctcggtact gctgataaag ccgacctacc
    10921 gcaaagcaat gtctccagcc caatgatggc ccagtcgctc aggcaaccag atctgcaacc
    10981 gatatcgacg gacaggaaga cagaatgttt tcgcctttac acaccagatc gcaaaccggg
    11041 ggtgaactgc gttcctgatg gctcaacagg ccattaagaa tgagggtgcc agtgattgcg
    11101 aaaactggca cctttcccat gacttagcgg aaattgacgc tacggtcgct ggtcatgtca
    11161 tacaactgga acttacgccc aagttgctga ccaccgtcac gcgtcagcgg cgtccagcca
    11221 atcgccgcac ggctgcgtgt cgggccagac gagaagagat ccaacggtac cgagacatac
    11281 acgcctttgg tgaagtcccc ttcgccgtac tcctcttttg aaacattagt gatcgtggca
    11341 tagccaccca ccaccacgcc gctatcaaag cgtttggcga tctccagcgt gccgccttta
    11401 tcccctgcca gatactgccc gacgctggct ttaactaaca catcctgagc gaaagatggc
    11461 gtccagtagg cggtcagatg tccggttttc acgctgtagt cggtgaattt catcatatct
    11521 tttgcactac gccagtcgcg ctgtttaacg tagttggcat ccagaccaaa cgcccagttg
    11581 ctgtccagcg ggcgatacaa cacttctgcc cccgcaccgc caaacatggt ttcgagataa
    11641 ccaccgtaga cctgaccgta gaagccgttg cccagatgct ggaagtagtt ggcttgcagg
    11701 ttattcacat agacatcgtt ctgcacatac tcgcgcacat gggtacgcac gcgcggtaag
    11761 tgcgagtcct gcggaggatt agtgtagtta aacttgtcgt agttgttggc aagatttgca
    11821 aacaggctgc cggtcgtcag cagatggtcc gtcagccaca aatctgccgt tcccatcacg
    11881 cccagctgat acatgtaaaa gttttccggg ccaccgaccg actggttcag caccggatcg
    11941 atatggaaat caaagcgtga tttgtcgata taccatccct gctcggtgga ctgcggaacc
    12001 actggctcga cgcgtttttg cgccagcgtc gtttcgtggc ccaacggctc tccggcgaga
    12061 tgatttttca ggctggcgac atcggtttcc gtcgtcacct gcggcatgtt aaggcgattt
    12121 tccgtaacgc ggatcgtttt gatcccatcc ggcagatcat tcatcacgat ccgattagca
    12181 cggatgatcc cttcgcgcga atcacgatat ttcacctgct cgccggtaac atacagcgta
    12241 tcgcctttcg cctggatctg cggatcggca agtccggcat tgtatttcaa cagcgttaac
    12301 tgattcgcca ccaccgaatg ctgcaaaatg gcatcctgcg gctgcggttg atattgcggg
    12361 cgggcgttat cgttgtaaga cgggcgcaga tcgttaaagt tggtgcgcag cgtgacgcca
    12421 aacataaagg tgttgccacg ttcatagcta aggttaacgt cggcccaatc ggtaacgcga
    12481 taaatcgcac cgacgttaaa cttacttttt tgctccagct tcccggcaaa atcctgctga
    12541 taattattgc cttcatactc cagtttcagg cgcagcggtt gccagggcgt ctggtattcc
    12601 acgccgccaa acagtgaggc aggaccgtgg aacatctggc taccgtcgat agatcccgcc
    12661 tgtttgtagc tgttatcgcg atagcaatat ttatcactgg ctgaacagag cggatttttt
    12721 acattaccgc tggtgcccag atatccccag cccaggccga gcgtaaaatc gaacggcccc
    12781 caggctttgc tggcaacaag atattccgca tcaaacagcc ccgtaccgcc gatatcccgc
    12841 gcaccaaccg ccacttgcgg cagccagtaa ctctcttccc acaaacgcag tttgagatcg
    12901 aaggctttat ctttatacgt ttgatcgcca gagaacgctt cgacgctgct gtactgccgg
    12961 gtgcgcacgt cggtgtagcg cagcgttgtt tccagccacg ggaagagttg cactgaagct
    13021 gagtaataac ggtactgatc gttatcgcga tagttcagac tcaactcccc ttcccgtgcc
    13081 atacgcgcgg tgggagtttg taataatcct acgccaccga aatccgactg cgacggacca
    13141 atgggcgcag gatatgtttc gccgtagcaa gctgtgctaa tgcccagcgc cagtaagcta
    13201 agcagatgtc tttttttcat tattggggta tccgctgcgt cagagtctga agaatgtcgg
    13261 cgttaagggc atcaggcgtc tcactccaga cggagtccgc gaggccaaca taaataatgc
    13321 tgccgggcat cggctctacg tgacgcttgt tccagtaagc caccggcgct ttttgcgtgc
    13381 gtccgtccgg gtaaaccacc cacgcgtagc tgcgatccgc accgctaagc aggttctgcc
    13441 cggagagata gctcgccacg tcgcgacctg gagtgaatgg ctgcttgcca ggacggctga
    13501 taagcccgaa cagcgtgacc gtggacggtg gtggcccgac ccacagcgtg tagttgccct
    13561 gcaacggtgg gttaccgcgt tcggcaacgc ggacgatatc gggatcaaga ttgatttttt
    13621 gcctgcccgt cactttcaac gcctgaattt gctggcgtaa ggcgttaatc gcggcggcat
    13681 cgtcggcgct ggaatctgca gccagttctg ccagccgtgt cagcaacgcc tgctgctgcc
    13741 gcaatgctgc cgccgttgcc agctcttcgc taatcaccgc accaggccac caactgtttg
    13801 ccagccgcgg ctggcctacc agatcgatta aatactctgc gcccgtcagc gttttggcct
    13861 cactgctgcc attgctgaat accttgacgg ttcccgccgc gaagaccgat gacgccccca
    13921 cactcaaaat caatgcgaca atagtttgtt taatcattgt tttgccgcct tgatgagagt
    13981 ggttttcacc gggaaataat ccgcgccgag atactgttcc gactggcgaa tttgcccttc
    14041 gctgtcgatc cagtagcggt tatgccagcg cgcctggtcg gtggagactt cttcgtccag
    14101 cacgcgaacc ggggtttcat cgctgccgac cttgacggta tcggtgccat cccatttgaa
    14161 gactgagcgt gcggtggcgt agcgtacctg ctggtactcg gtccagccca tcgtgcgcgt
    14221 ccatgttgca ccatcaacaa tttgtgcggg tttaatcagc gggtcggcgg caaggttatt
    14281 cacttcaatc aggttgtcgc cgccaagcaa agtcttcacc agacgaccat gttgtatgac
    14341 aagagtggct tgatcctgag tgacccattt ttgttgtccg tcttcagcga aggccagcac
    14401 cacaaacaat tgcggcccgc cattaagctg catgtactgg ctggcgtagg gaatattttg
    14461 aatatcatcg tccgtcagct gtacgcctgg cgtgccgaac agactgtccc acagtgaatt
    14521 gcccagctct ttagtcgtgg ctgaacaggc ctgtaatagc aggcagataa gaatgagtgc
    14581 aggtcgcttc acgactcttc tccagagggg cagaataacc acaccggagt gtggttatgg
    14641 taaatccctt catacttcaa gccacagatg caatgactca aattattttg gggatacata
    14701 ctttattact gggtactggt ggtcgtggtt gtggtggtcc cggtattaga accatcaccg
    14761 ccaccggttg ccgcgagcgc gacacccacc gccgagctta cggtgctgac gctggtcgcg
    14821 gaggaactcc ccgccgaaac cgacgttgct gccgaccctg ccgcttcgcc cacctgtaca
    14881 ggtgcagccc acgcagaagt cgccgcaagc gcagatatgg caaaaatgcc atacagaact
    14941 tttttcataa caatttccct tcattgaatg aatggagatt tacccaaaaa catttcgggc
    15001 gggatcgagt atacacaact aaagcatgcg aattacggcg cggggaatta gagatggggt
    15061 tttaggataa ttggaatgaa tatcaaatat aagctacaac ttatatataa ataacattaa
    15121 aaatcataat gttaaaataa aagtaaaagt aattaatctg agtattttcc gaaatcaatc
    15181 acgattaaaa atctatttta ggattaacct cacggtatga tttccgttaa atcacagaca
    15241 aggggattta tctgataaaa caatgccgac ataacgtcgg cattgttatt aaggttttta
    15301 aattaatcgc gccacgcttt atagcggtta atcagaccat tggtcgagct atcgtggctg
    15361 ctgatttctt tatcatcttt cagctctggc agaatacggt ttgccagttg tttacccagc
    15421 tccacgcccc actggtcgaa ggtgaagatg ttcaggatca cgccctgagt aaagattttg
    15481 tgctcataca gcgcaatcaa cgcccccagg ctgaacggag tgatttcacg cagcaggatg
    15541 gagttggtcg ggcggttacc ttcgaacact ttgaacggca ccacgtagtc aagcgttgcc
    15601 ggatctttac cctgatcgcg atattcctgc tcaaccactt cgcgggattt accaaacgcc
    15661 agcgcttcag tctgagcaaa gaagttagac agcagtttct ggtggtgatc agagagcggg
    15721 ttatgggtga tagccggagc gatgaaatcg cacggtacca ttttggttcc ctggtggatc
    15781 agctggtaga acgcgtgctg accgttagtg cctggttcac cccagataat cgggccagtc
    15841 tggtaatcca caacgttacc gttacggtca acatacttac cgttggactc catattgccc
    15901 tgctggaagt acgccgcgaa acggtgcata tactggtcat acggcagaat cgcctcagtt
    15961 tccgcaccaa agaaattgtt gtaccagatg ccaatcagcg ccagcagtac aggcaggttt
    16021 ttctcggcag gcgtggtgga gaaatgcttg tccatcgcgt gtgcgccaga aagcagttca
    16081 acgaagttat caaagccgat ggagagaaca atcgacaggc caatcgctga ccacaaagag
    16141 taacggccgc cgacccagtc ccagaactcg aacatgttgg cagtatcaat accaaactcg
    16201 ccaacggctt tggcattggt tgagagcgcc gcaaagtgtt tcgcaacgtg tttttcatca
    16261 cctgccgctt tcaggaacca gtcacgcgcg ctatgggcgt tggtcatggt ttcctgagtg
    16321 gtgaaggttt tagatgctac caggaacagc gtggtttccg ggtttacttt tttcagcact
    16381 tccgcgatgt gagtcccatc gacgttagaa acaaagtgca tgttcaggtg gtttttgtac
    16441 ggacgcagtg cttcggtcac catgtacggg ccgaggtcag aaccgccgat cccgatgttc
    16501 actacgtcag tgattgcttt gccggtataa cccttccact caccggagat aatcgcttcc
    16561 gagaaggttt tcatcttctc cagcaccgcg ttgacttccg gcattacgtc tttgccatca
    16621 accaaaatcg gggtattgct acggttacgc agcgctacgt gcagcacagc gcggttttca
    16681 gtgcggttga tcttctcgcc agagaacatc gacttaatcg cgcccgccag atcgcactct
    16741 ttcgccagat cttgtaattt cgccagcgtc tcttcagtga tgcggttttt ggagtaatcc
    16801 accagcatct gatcgccgaa ggttgcggag aacttagaaa aacgatcgcc gtctttagca
    16861 aaaagatcgg cgatcgtaac gtctttcatt tcatcgaagt gtttctgtag tgcctgccag
    16921 gcagcggtct gcgttggatt gatgtttttc attagcaata ctcttctgat tttgagaatt
    16981 gtgactttgg aagattgtag cgccagtcac agaaaaatgt gatggtttta gtgccgttag
    17041 cgtaatgttg agtgtaaacc cttagcgcag tgaagcattt attagctgaa ctactgaccg
    17101 ccaggagtgg atgaaaaatc cgcatgaccc cgtcgttgac aaccgccccg ctcacccttt
    17161 atttataaat gtactacctg cgctagcgca ggccagaaga ggcgcgttgc ccaagtaacg
    17221 gtgttggagg agccagtcct gtgataacac ctgagggggt gcatcgccga ggtgattgaa
    17281 cggctggcca cgttcatcat cggctacagg ggctgaatcc cctgggttgt caccagaagc
    17341 gttcgcagtc gggcgtttcg caagtggtgg agcacttctg ggtgaaaata gtagcgaagt
    17401 atcgctctgc gcccacccgt cttccgctct tcccttgtgc caaggctgaa aatggatccc
    17461 ctgacacgag gtagttatgt ctgaaattgt tgtctccaaa tttggcggta ccagcgtagc
    17521 tgattttgac gccatgaacc gcagcgctga tattgtgctt tctgatgcca acgtgcgttt
    17581 agtagtcctc tcggcttctg ctggtatcac taatctgctg gtcgctttag cggaagggct
    17641 ggaacctggc gagcgattcg aaaaactcga cgctattcgc aacatccagt ttgccattct
    17701 ggaacgtctg cgttacccga acgttatccg tgaagagatt gaacgtctgc tggagaacat
    17761 tactgttctg gcagaagcgg cggcgctggc aacgtctccg gcgctgacag atgagctggt
    17821 cagccacggc gagctgatgt cgaccctgct gtttgtcgaa atcctgcgcg aacgcgatgt
    17881 tcaggcacag tggtttgatg tacgtaaagt gatgcgtacc aacgaccgat ttggtcgtgc
    17941 agagccagat gtagccgcgc tggcggaact ggccgcgctg cagctgctcc cacgcctcaa
    18001 tgacggctta gtgatcaccc agggatttat cggtagcgaa aataaaggtc gtacaacgac
    18061 gcttggccgt ggaggcagcg attatacggc agccttgctg gcggaggctt tacacgcatc
    18121 tcgtgttgat atctggaccg acgtcccggg catctacacc accgatccac gcgtagtttc
    18181 cgcagcaaaa cgcattgatg aaatcgcgtt tgccgaagcg gcagagatgg caacttttgg
    18241 tgcaaaagta ctgcatccgg caacgttgct acccgcagta cgcagcgata tcccggtctt
    18301 tgtcggctcc agcaaagacc cacgcgcagg tggtacgctg gtgtgcaata aaactgaaaa
    18361 tccgccgctg ttccgcgcgc tggcgcttcg tcgcaatcag actctgctca ctttgcacag
    18421 cctgaatatg ctgcattctc gcggcttcct cgcggaagtt ttcggcatcc tcgcgcggca
    18481 taatatttcg gtagacttaa tcaccacgtc agaagtgagc gtggcattaa cccttgatac
    18541 caccggttca acctccactg gcgatacgtt gctgacgcaa tctctgctga tggagctttc
    18601 cgcactgtgc cgggtggaag tagaagaagg tctggcgctg gtcgcgttga ttggcaatga
    18661 cctgtcaaaa gcctgcggcg ttggcaaaga ggtattcggc gtactggaac cgttcaacat
    18721 tcgcatgatt tgttacggcg catccagcca taacctgtgc ttcctggtgc ccggcgaaga
    18781 tgccgagcag gtggtgcaga agctgcattt taatttgttt gagtaatatt tccctactgg
    18841 cgtataacgc tcaccgattg ttatacgcca tcctcactga aaacatagat taaataatat
    18901 ctgaaaagca aaatcactcc tgtagaacag tattataatc accgaaaaat atcatttaac
    18961 aggagtgtta atggctactt taatggaaaa agatgcactt ttaaacggag ccagtcagtg
    19021 catcgcgttt ttatcaaata tcattgataa ttgctcagtt tcgtcgcatc aggattcagg
    19081 cgatgctctc aagcgattag tcagctatcg cgattatctt tattctaccc ccgctgaact
    19141 tgttgatttt acacaaggta agattctcct tcagcaggta agaactcaat atcagcatga
    19201 attcaacaat actacgcatt cagaaaataa agcatctttt gattctattt ggcagcgtct
    19261 gacaaatcat gaagtcacac cgcaacagca tcccattggt tttgttttag gtgggcaacc
    19321 cggagcaggt aaatcatctc tgattgaatt agccaaaaga gagacaaaag ataatataat
    19381 gattattaac ggtgatgatt ttcgatttct acaccctgat tttaattata tctaccaaaa
    19441 ttatggggat gattttgtta cccatacagc gaaattttcc ggcgaaacgg tcgaacgtgc
    19501 aattgagaga gcgatagtca gtaaactcaa cattgttgtc gaaggaacat ttcgtaatgc
    19561 cgcgacgccg ttacaaacat taaaaaaact caaagatgca ggctaccaaa cagaagtcat
    19621 gataaaaaca acctcagcgg cattaagctg ggaaagcacc aatgaacggt ataataagga
    19681 caaagaggcg ggaaatattg cccgaaaagt ggataaaaac caccatgata tcgtcaccgg
    19741 tttgctggca gaaaatgccc gcaaggtgtt tgcttcgaac ctgtcagata agtttgcggt
    19801 gtattcgcgt gagaagatga tatttagttc acaagcggca acgaatgacg atattgcaac
    19861 acttatacag aatgaaatca gcgggaatac acaataataa taagagctga tggcgtgaaa
    19921 taacgccggg ctttatgccc ggcgcattac cgtctggtac attaagctat ccagttcctt
    19981 aacgtcccct tctctgctat ccggtaacag cggtggaatt tccccacgcg ccagttccgc
    20041 acgaataaac gcatgtaaca atggacggcg cggaccatat tccgcttctc ctgcgcgctg
    20101 tttacgttcc agtaattcgt ctatttctgc acgtaacggc gcatcaagtt cgcttcccgc
    20161 cagtaactca gcaaagcgca ttggcggtac gcctttaccc gcttcgaccc agcgcacggc
    20221 cagcagcgga cgcagcacgt aaaagtattt tttcagtcga acttcgtcgc cctgcaaata
    20281 tccggggaag tttttctgcg ccatcgaata gtaatgccag cgcgcacgca gcggagaaaa
    20341 ccacattggg accattgcct taaacgccgt tatcgtttct tcatcttgtt gataaatcac
    20401 cggagaatcc agccattcta tcagcgtcgg gttcgcgcct ttcaataatc ccagcgcctt
    20461 acgccactcc caaccggaaa catctaactc gtcgtcgatg ggcagctcaa taacatcgcg
    20521 cggtgactct actcgcagat accattccag cgggtgaaca tacaaaaacc gtacatcgta
    20581 gtcgctgtcg ggcgaggcaa atccccagcc gcggctccct gattcacagg cgtacagtac
    20641 tttcacatca taccgccgtt caatttcttt taactgctgc gatacccttt cgcgcatcgc
    20701 agcactgact ccatttaatg gcatgttact caccctttga tattaaccaa cccatgcaga
    20761 acctgatcca gaccgccaaa tacagagatg ttatcgatcc gctcagccac tctctccagc
    20821 gtttctagct cttttaaacg caacgctacc ggattgttct ccatgacttt ggcggtgttc
    20881 agtaacgagc gcgtcgctgc cgtttcttca cgtcggcgaa tcacattcgc ctgcgcagat
    20941 ttctctgcct caaccagctg cgcgaggata tttttcatat caccaggcag gacgatatct
    21001 ttcaccccta atgaggcaat ctccatacca aatggcaaca tgcgcgattt cacctgttcg
    21061 ctgacaacgt catcaatgac ctgtttatct tccagcagtt catccagcgt acgagtacct
    21121 accgcttcac gcagggcaaa ttgcaattcc cgatacaaat gatcaatcgg cttcgttaac
    21181 tgactaaatg ccagcaacac gtcgctgtag cgccagttag cggcgaggtt aatccgcaga
    21241 ttgaccttat ctttcgttaa gatttcttgc ccgctgactt ccagtacctg caagcgggtg
    21301 tcaaccaatt cagcgtcaac cagatggtta attttccagt atgcggtcag ccccggcggt
    21361 aacagcgcct gcgtttcacc atcgattttc agcacgccta catgccaggc cggaacctgc
    21421 accgttagta ttgcatcgcg gcctttaact gcccctttgc gccgtggttg caataccgca
    21481 ttcatcacct ccgtttgtac ctggacttgc tgcgtattca tccgcaccag tgtcaaatca
    21541 tcctctacac gccagtacaa acggcgcgtt gatggcggca ggatctcctg caaaataccg
    21601 tccatataca acgccccggc ttcaatctcc gacaagtctg ccaccaggca atatttctcc
    21661 acccaatcag gctgaaagcg gcgcaaataa tccgccagca cgtcgggaac ttcgctgcca
    21721 tcgagcgtaa tcagcaagac ttctggcgta tttaaccagg gtaaaagatg ctcgcctgca
    21781 tgtagcactt tgtagtaatc gccgtttctg gagagtaatg ccagctgatc ttttcttacg
    21841 cttatttttt taatcattat ctattccttg cttaacagcc gtcgcggaag cagacacgaa
    21901 gcgacagggg aattcccttt tgcctgttac ccggcatctt tatcggcgac gttatcgggg
    21961 atcgctgact gcgcaatccc tcacttttta tttaataagt taaaaccagt attcatttca
    22021 ggaatgaatg tgggaatcga acccataatt ggcgtcaaag gctgacggaa cacgcccgga
    22081 aaacagcctg gccgttcttg gtgtgccgac ggggtttgac cccctttgta attcagtttc
    22141 aacgcgtacg agatattgcg agaagcgtgc cagaattgag aaaaaattat taaccaaatg
    22201 aaaaataaag atttaataaa ttatttttgt ttgtagaggg aaaacttttc gctaaatttg
    22261 tatctgtatt tatcgtcaat tatcaagaaa gataaatatt taagaagaga acaaaggaat
    22321 attatcagtt cagctcgcaa acgaattccc gtacactgaa ctctctggct cactgagggc
    22381 accatcatgg cactcccccg tattacccaa aaagagatga ctgaacgcga gcagcgtgaa
    22441 cttaaaacac tgctggatcg ggcgcggatt gcgcatggtc gcgtactgac caactccgaa
    22501 accaacagca ttaagaaaga gtacatcgat aaattaatgg tcgagcgtga ggctgaagcg
    22561 aaaaaagccc gccagttgaa gaaaaagcag gcttataaac cggatcctga agcgtcattt
    22621 agctggtcag caaatacgtc gacgcgcggc aggcgctgat tagcgcccct tctttttacg
    22681 ccccggagag gtaaaacgct taccgttgga cgccgggcga ccgccttttt ccgccgtttt
    22741 ttccatctta acgactggac gtttgatgcc cgctgttttc ggtttggcct tcactttagg
    22801 ttttgcctcg gaagaggaat tttcgatgag cttaaagagg tcgattaact catcatcagt
    22861 taaatcgcgc cactcaccca gcggaatgcc gcttaaactg acgttcataa tgcgcgtacg
    22921 ttccagcttt ttcacttcgt aaccaaagtg ctcgcacatg cgacggatct gacggttcag
    22981 cccctgcacc agggtaatgc ggaagacaaa cggcgcttct tttttgactt tacacttttt
    23041 agtcactgtc ccgaggattg gcacccccgc ccccatacca cgaataaatt catcggtaat
    23101 cggtttatcg accgtaacca gatactcttt ctcatgatcg ttacccgcac ggaggatctt
    23161 attcaccaga tcgccgtgat tggtgaggaa aatcagcccc tgggagtctt tatccaggcg
    23221 gccaatcggg aacacgcgtt tgctgtggtt aacgaaatcg acaatgttat cgcgctcgcc
    23281 atcttcggtg gtgctgacaa taccgacggg cttgttcagg gcgataagta ccaaatcttc
    23341 ggcttcccga ggctcaatca actgaccgtt tactttcaca acgtcgccgg gtttcacctg
    23401 atcgccaatg gtggctcgct tgccattaag gaacacattg ccttgctcga tatagcgatc
    23461 cgcttcgcgg cgtgagcaaa ttccgctttc gctgatgtat ttatttaaac ggactgatga
    23521 gtcgggcagc ataaattctc ctgtaaaaaa cggaatatac cgtacctttg ggttgataaa
    23581 aaatagtcat gagcgaacta tttgttattt ccattggctt gaattcgtta cctgctcaaa
    23641 tcactttcca gaagcaatgt gcgacatcgt gagccatgtc agtaagtggt taaattcgca
    23701 gtggtagcat gccttccgtg atttcttact aaccggagaa gtaatggaac tgcttttatt
    23761 gagtaactcg acgctgccgg gtaaagcctg gctggaacat gcactgccgc taattgctga
    23821 acagttgcag ggtcgccgct cagcggtgtt tatccctttc gctggcgtaa cgcagacctg
    23881 ggatgattac acagcgaaaa cggctgcggt tctcgctccg ctgggtgttt ctgtcaccgg
    23941 tattcatagc gttgtcgatc ccgttgccgc gattgaaaat gctgagatcg tgattgtcgg
    24001 gggcgggaat actttccagt tgctgaaaca gtgccgcgag cgcgggctgc tggcaccaat
    24061 tactgacgtg gttaaacgtg gtgcgctgta tattggctgg agcgcaggcg ctaaccttgc
    24121 ttgcccaacc attcgtacca ccaacgatat gccgattgtc gatccgcaag gtttcgatgc
    24181 gctaaatctg ttcccgctgc aaatcaaccc gcacttcacc aacgcgctac cggaaggcca
    24241 taaaggtgaa acccgtgagc agcgtattcg cgaactgctg gtcgtcgcgc cagaactgac
    24301 gattattggt ctaccggaag gtaactggat cacagtgagt aaaggtcacg ctacgctggg
    24361 tggcccgaac accacttatg tgtttaaggc tggtgaagaa gcggtgccgc tagaagctgg
    24421 tcaccgtttt taagtaaatt gccggatgca tgatccggca tcttgttcat agtgcctgat
    24481 gcgacgcttg acgcgtctta tcaggcctac aggattcaca atattaatac tcgtcccgtc
    24541 cttcatcttc atccggctgt tccagcacac tgtaagccac cgaacaaaac agcgagttca
    24601 ggcgctgcat atctcccagt aagcctaaat gtagcgaact ggtttcaatg ctctgcacgt
    24661 tttgctgatg caggcgatcg acgtgggcgt gggaatagcg gcgattaaga atgcgaaaac
    24721 gatgtttgct gcgacgcaaa cgacgagcgc tggtgacatc gccagagaag aacaccgaca
    24781 ttgccagctt taaattactg agcaattgct catagagcgc atccagttcc ttcaacccat
    24841 caagcgaaaa tgcccgccgt gctgccagcg atttatcggc aatttcgctg cccatgcgct
    24901 cgacgatatc ggaagcctgt tcaaggttga gcgacatttc aataatctcc gcccagcgac
    24961 gcgactcttc ttctgccagt tcctcttttg gcatccgcgc cagatacagc ttaatggcgg
    25021 tatagagaac gttgatatca tctgccagct tacgcagctc tttctcctgc cgtggctcgc
    25081 cgtgcatcac tttattcagc ccttccatca tctgttccat ggcgtcgcca atgcgcaggg
    25141 tttcgcgcgc ggcgttcgcc agagcaagcg tgggcgtatc cagcgcgctg acatccagat
    25201 gtttaggccg tagctgggta tccagttccg gttcatcgcg aataatcgtt ttgcaaaacc
    25261 gtgccatcgg gtcaacaaat ggcagcatga ccaggcaacg tacaaggttg tagaagacgt
    25321 ggaaatagat caccagttcc gcttttggca atgacagctt ccccattgtc tctgccagca
    25381 aatggacaaa cggcaggata atcaggctac ccaccagctt aaacagcaga ctacccagcg
    25441 cgacacggcg ggctgcggca ttggcggcac tgttgttgag catcgccagc aggccggaac
    25501 cgaggttagc accaatcacc agacagagcg ccacggggaa ggagataatg cctgcggcgg
    25561 tcagagtcgc agtcagcagt acagcagcaa ggctggagta gctgataatg gcgaacatcg
    25621 cgccaatcag cgcatccagc agaatatcgc cggtcagcga ggcaaagatc acctgaacgc
    25681 cgtttgcctg ggtgatcggc gttacggcct gcacaatcaa ctccagcgcc agtaaaatca
    25741 gcccaagacc aataccgacg cggcccagtt gcccggcgcg tgactgtttg cgtccgagga
    25801 aaaaaatcac gccgataaaa ataagtaacg gtgacagcca ggataagtcg aaggtgagaa
    25861 tacgcgccat tagcgccgtc ccgacatctg cccccagcac aatgaccaga gccggtgcga
    25921 gggctaccag atcctgagcg acaaacgagg tcaccagcat ggtggtggca ttactgctct
    25981 gcaccagtgc ggtaacgccg atccccgcgc aaaaggcgag cggctttttt tcgacgctcc
    26041 ggctaaggac agtacgcaaa cgcgcgccga agacgcgcat tacgccggtt cgaacaatat
    26101 gagtccccca gaccagcagg gcgacggcag aaagcaggtg aagcagcgtt aacacgagtt
    26161 caggttctcc ttatcgttat atgttcctga agcaattgaa tgaccaaagg accggaaaca
    26221 cgctgactgg catctttact gtgccttttg acattcgttt ttccggctaa gattattcca
    26281 gcctgtttcc agtataagtg tttcatatta ataaagagac agggcactca ttgagcgccc
    26341 tgtttgttgt aaggaaaagt gacagatttg tgaatcagtc cgcgtcatac cccagattcg
    26401 gtgccagcca gcgctcaaca tcggagacgc tcataccttt acggcgggca tagtcttcaa
    26461 cctgatcgcg ctgaatttgc gccaccgcgt agtacttgct gtccgggtgg ctgaaatacc
    26521 aacccgaaac cgatgcaccg ggccacatgg cgaaagattc tgtgagtttc atgccggtat
    26581 gtttttccac ttccagcagc tcccagatgg tggctttttc cgtatgttcc gggcaggccg
    26641 ggtagcccgg tgccggacgg atgccctggt agttttcgcg gatcagttct tcgttgctga
    26701 ggttctcgtt cggcgcatag ccccagtaga ctttacgcac acgctcatgg agatactccg
    26761 caaaggcttc ggctaaacgg tcggcaagcg ctttcaccat gattttgttg taatcatcgt
    26821 gctgcgcttc aaaggcatca gccagtgcgt cctcttccag cccgccagtc acggcaaatg
    26881 cgccgatgta atctgcttta ccagaaagct tcggcgcaac gaagtcagcg agacagtagt
    26941 tagcgaatcc cgttttttcg gtctgttgac gcagatggtg gctgacgttg atcacatggg
    27001 tgcgcgtttc gtcacggtag atttcaatgt catcgcccac acggtttgcc gggaacaggc
    27061 ccaccacgcc acgcggattc agcgttttct cggcgcttaa tttatccagc atgtcgttgg
    27121 cgtctttaaa cagccgctgc gcctcaacgc ccaccacttc atcttccaga atgcgcggat
    27181 acttcccggc cagcgaccag gtcataaaga acggtgtcca gtcgatgtaa ttacgcagag
    27241 tttcgatgct ggcttcaact tcctgcacgc cgagacggtg cgccaccggc ggcgtgtaag
    27301 cctgccagtc aaaagcgaaa tcgttatcgc gcgccgcttc cagcgtgacc ggtggtgtgc
    27361 gcggtttctt acgcccgtgc tgaatacgta cggtttcgta ctccttgcgg gtacgagcga
    27421 caaaatcatc acgctgagta tcggaaagca gcgccgccac cacaccaacg gtgcgcgatg
    27481 cgttctgtac atacaccgtc gggccgctgt agttctgctc gattttcacc gccgtgtgcg
    27541 cttttgaggt cgtcgcgccg ccaatcaata gcgggatggt gaagccctga cgctccatct
    27601 ctttcgccac gttaaccatc tcgtccagcg acggcgtgat aagccccgaa agtccaatca
    27661 gatcagcatt cacttcttta gcggtacgga gaattttttc cgcaggcacc ataacgccga
    27721 gatcgacaat ttcgtagttg ttacattgca gcaccacacc aacgatattt ttaccgatgt
    27781 cgtggacgtc gcccttcacg gtggcgatca ccatcttgcc gttggttttg ccctgctctt
    27841 tgctggcttc aataaacggt tcgagatacg ccaccgcctg tttcatgacg cgcgccgatt
    27901 tgaccacctg tggcaggaac attttccctt cgccaaacag atcgccgacc acgttcatgc
    27961 cgtccatcaa cgggccttca atcacttcaa tcgggcgcgt agcctgctgg cgggcttctt
    28021 cggtatcctg ctcgataaac tcggtaatgc ctttgaccag cgagtattcc agacgtttat
    28081 tcacttccca cgagcgccac tccgcctgct gggcgttagc ggtgtcgtcg gttttgctgc
    28141 cgcgatattt ctcggcaagc tccagtaaac gctcagtgcc atcatcgcga cgattaagaa
    28201 tcacatcttc caccgcgtcg cgcagttcag cgggcaggtc gtcgtaaatc gccagctgcc
    28261 cggcattgac gatccccata tccatgccat tgcgaatagc gtagtagagg aacactgcgt
    28321 gaatggcttc gcgcaccgga tcgttgccac ggaacgagaa agaaacgtta gatacgccgc
    28381 cggaaattag cgcgtgcggc agttcgcgtt tgatgtcttc acacgcgccg ataaagtcct
    28441 gcgcgtagtt gttgtgctct tcaatgccgg tggcaaccgc gaagatgttc gggtcgaaga
    28501 tgatatcttc tggcgggaag ccaacctctt cggtgaggat tttgtacgcc cgacggcaaa
    28561 tctcgatttt ccgtgcgcgg gtatcggcct gtccctgttc gtcaaaggcc attaccacca
    28621 ctgccgcacc gtaacgacgc aagagcttcg cgtgatggat aaaggcgtcg acgccctctt
    28681 tcatcgagat agagttaaca atgcctttgc cctggataca cttcagacct ttttcaatga
    28741 catcccattt tgaggagtcg atcataatcg gcacgcgagc gatatccggt tcaccggcaa
    28801 tcagattgag aaaacgcacc atcgccgctt cggcgtcgag catcccttca tccatgttga
    28861 tatcgataat ctgcgcaccg ttttccacct gctggcgggc cacatccagt gcctcgctgt
    28921 atttctcttc tttgatcagg cgcttgaact tagcggaacc ggtgacgttg gtgcgttcac
    28981 ccacgttcac aaacaggcta tcttcgccaa tgttcagcgg ctccaggccg gacaaacggc
    29041 aggctacggg gatttccggc agtttgcgag gcgctaatcc ttctactgca cgactcatcg
    29101 ctgcaatatg ttgtggcgtg gtgccacagc agccgccgac gatattgaga aaacccgctt
    29161 gcgcccattc acgtatctgt tttgccatcg tgtcggcgtc gagatcgtac tcaccaaagg
    29221 cgttgggtag cccggcgttc gggtgcgcgg tgacgtagca ttccgcaatc cgtgacagct
    29281 cctgcacgta ctggcgcagt tcatcgggcc ccagcgcaca gttcaggcca aaggtcagag
    29341 cttcggcgtg gcgcaatgag ttgtaaaatg cttcggtggt ctgcccggag agcgtgcgcc
    29401 cggaggcgtc ggtgatggtg ccggagatca taatcggcag ctcaacgccc agcgcttcaa
    29461 actccgtttt caccgcaaaa accgccgctt tggcgttaag ggtgtcgaaa acggtttcaa
    29521 tcaggatcag atccgcacca ccttccacca gcgctttggt ggattcgcga taggccgcca
    29581 ccagctggtc aaaagtgatg ttgcggaatg ccggatcgtt gacgtccgga gaaatagacg
    29641 ccgtgcggtt ggttgggccg agaacaccgg caacgtagcg cggtttctcc ggcgtgcgcg
    29701 cggtccactc gtcagcacag gcgcgcgcca gtttcgccgc cgcaaagttg atttccgccg
    29761 acagggattc catctggtaa tccgccatcg caatggtcgt ggagttgaag gtgttggttt
    29821 cgatgatatc cgcgcccgct tcaaagtagg cgttgtggat agcggcgatc acttccggtt
    29881 tgctgagcac cagcaggtcg ttgttgcctt tgagatcgca tggccagtcg gcaaagcgtt
    29941 caccacgaaa atcggcttcg ttcagtcgat agctctggat catggtgccc ataccgccgt
    30001 ccagcaccag aatacgttca tttaactgcg cacgcagttg ttccactttg ctgctcacac
    30061 ttgctcccga cacgctcaac ccagatttaa taaaaattca acaaaccata ctggcataaa
    30121 cgcatctgtg gtaaaagcga ccaccacgca acatgagatt tgttcaacat taactcatcg
    30181 gatcagttca gcaactattg cattagctaa caataaaaat gaaaatgatt tccacgatac
    30241 agaaaaagga ggctgtcatg gtcgcaccca ttcccgcgaa acgcggcaga aaacccgccg
    30301 ttgccaccgc accagcgatt ggacaggttc agtctttaac gcgtggcctg aaattactgg
    30361 agtggattgc cgaatccaat ggcagtgtgg cactcacaga gctggcgcaa caagccgggt
    30421 tacccaattc cacgacccac cgcctgctaa ccacgatgca acagcagggt ttcgtgcgtc
    30481 aggtcggcga actgggacat tgggcaatcg gcgcacatgc ctttatggtc ggcagcagct
    30541 ttctccagag ccgtaatttg ttagcgattg ttcaccctat cctgcgcaat ttaatggaag
    30601 agtctggcga aacggtcaat atggcggtgc ttgatcaaag cgatcacgaa gcgattatta
    30661 tcgaccaggt acagtgtacg catctgatgc gaatgtccgc gcctatcggc ggtaaattgc
    30721 cgatgcacgc ttccggtgcg ggtaaagcct ttttagccca actgagcgaa gaacaggtga
    30781 cgaagctgct gcaccgcaaa gggttacatg cctataccca cgcaacgctg gtgtctcctg
    30841 tgcatttaaa agaagatctc gcccaaacgc gcaaacgggg ttattcattt gacgatgagg
    30901 aacatgcact ggggctacgt tgccttgcag cgtgtatttt cgatgagcac cgcgaaccgt
    30961 ttgccgcaat ttcaatttcc ggaccgattt cacgtattac cgatgaccgc gtgaccgagt
    31021 ttggcgcgat ggtgattaaa gcggcgaagg aagtgacgct ggcgtacggt ggaatgcgct
    31081 gactttttct ggcgggcaga ggcaatattc tgcccatcat acctgagtgg caatagaata
    31141 agggtgtctg ttaatcgcat tgacgccaaa ataacttaat gtcatacact tcacacactt
    31201 ttattaactc agcattattt ttaaacatca aactacttaa tcattaacaa aatcgcaaga
    31261 tctcattcca ccgaaacttt tacggctgca ttatgagatt gagcgattta tgatatttct
    31321 ttagttgtaa tcatattaaa ttttttcact gttctgatag ttaaaattca agacatcaat
    31381 aaacaatgag atatttaaat gattactcgt atccctcgta gttctttctc tgcaaatatt
    31441 aataatacag cccagacaaa tgaacaccaa accctgagtg aattgtttta taaagaactc
    31501 gaggataaat tttctggcaa ggagctggcg acgcctctat taaaaagctt ctcagagaac
    31561 tgtagacaaa atggtcgtca tatctttagc aacaaggatt ttgtcattaa attttccacg
    31621 tccgtcttac aagctgataa gaaagaaatt acgataatta ataaaaacga aaacacgaca
    31681 cttactcaaa ccattgcccc aatatttgaa gaatacctaa tggaaatttt acctcaacgc
    31741 tcagacactc ttgataaaca agaattaaac ctaaaatcag atagaaaaga aaaagaattc
    31801 ccaagaatta aacttaatgg tcaatgttat tttccggggc gaccccaaaa ccgtatagta
    31861 tgccgacaca ttgctgcaca atatattaat gatatttatc agaatgttga ttacaaaccc
    31921 catcaagatg attactcttc agctgaaaaa tttctcacgc acttcaacaa aaaatgcaaa
    31981 aaccagactt tggcgttggt ttccagccgt cctgaggggc gttgcgttgc tgcctgcggt
    32041 gatttcgggc tagttatgaa agcatatttt gacaagatgg aatcaaatgg catcagtgtt
    32101 atggcagcca tattactggt ggataaccat gctttgacgg tccggctaag aataaagaac
    32161 acaactgaag gatgtaccca ttacgtggtt tcggtttatg atcctaatgt aactaacgat
    32221 aaaataagaa ttatgagcga aagcaaagag gatattaaac actattctct gatggatttt
    32281 atgaatgtag attatagcct cctgaaatgg tcaaatgatc atgttattaa ccaatctgtt
    32341 gcaataattc cagcacttcc gaaagaacag ctattgatgt taaaaggatc tgtggatgaa
    32401 ataacccctc cattatcacc agcaacgatg aatttgctaa tggcaattgg tcagaatcac
    32461 caacttacgc aactgatgat tcagctccag aaaatgccag aactacatag aacagaaatg
    32521 ttgactgcct ataatagtgg acatatgaac gttattaata ctatttttaa cgcattaccc
    32581 actctgttta atacgtttaa attcgataaa aaaatatgaa gcccctcctc ctggcaaata
    32641 attctaatga atatcccggt ttgttttcag cgatacagca taaacaacaa aatgttgtag
    32701 agacggttta tcttgcttta tctaaccatg cacgcctgtt tggatttacc gctgaagata
    32761 ttatggattt ttggcaacac aaagccccac aaaaatactc tgcctttgag ttggcttttg
    32821 aattgggtca ccgggttatt gctgaattaa tccttaatac attaaataag atggctgaaa
    32881 gctttggctt tacggataac cctcgataca ttgcggagaa aaattatatg gaagctttac
    32941 tcaaaaaagc atctccccat accgtacgct aaatctttgc ctgcgccgat acgcataaac
    33001 atcttccaca tgcccttcac gtatgcggtt ttgtagtgcg cgccagtaat cagcgcggaa
    33061 caggtcggcg tgcatctctt caaacagcgg accaatacgc gggtcggcgc atagccagtg
    33121 gcgaaactct tccgggaaaa catcgcccgg cgagacgctg taccacggtt cgctggcaag
    33181 ttcgtcttcc ggatagcgcg gcggcgggat gtcgcggaaa ttcacttccg tcatgtagca
    33241 aatttcatcg taatcataaa aaaccacacg cccgtgacgg gtgacaccga agtttttaaa
    33301 cagcatgtcg ccaggaaaaa tgttagcagc ggcaagctgg cgaatagcgt taccgtattc
    33361 ttcaatggcg tcgcgcaact gctgaccttc cacttgttcc agccagatat tgagcggcac
    33421 catccgccgc tcgatataga gatggcgaat tacaatctgc tcaccaagat cggtgatttt
    33481 ttccgctgct tcctggagca gtaattccat taatgccggg gaaatatgcc gcttctccag
    33541 cacaaagttt tcaaactcct gggtgtccgc cattcggccc acacggtcgt gctctttcac
    33601 cagttgatag caggcacgaa cgtgagcggc agacatctct ttctgcggcg cgaacttgtc
    33661 tttgatgact ttgaataccc gatcaaagcc cggcagcgta aataccaaca tcaccattcc
    33721 acgaataccc ggcgcttcaa tgaactgctc attacagccc tgtagataaa cgagatattc
    33781 gcggtagctt tcggttttgg cgtgcttctg gcagccgata gccatataca attcagcagt
    33841 ggttttacct ggcagaattt cccgtagcca ctcgaccagc gctgcgggca gcggtgcata
    33901 aaccataaaa taagaacgcg caaagccaaa aacaatgctc gcttcggcgg tcgtcgtcag
    33961 gcaggtatca ataaataact cgccgtcgtc cgtctggtgg atcggcagta agaagggcaa
    34021 cgtgccggaa ggcgtgatca gtttgcccac cagccaggcg gctttattgc ggtaaaacag
    34081 ttcgttcgcc acctgtaaat gactttccgc gaggttgtct gtccccagcg tttccgtcag
    34141 atggcgaatg atgtaatgaa tgtcacggct tttattctgc cagcgcaggc gcagcggcag
    34201 gtcgctgata acgcgcatca gtagagattc ccagccgtga tcggggtgaa agtctttcgc
    34261 cagcgggcgg ggaatggtac gaaagcggcg ctctggctga gagctaaaga taaaaagccg
    34321 ctcgggagta agcgagcggt ggtcaaataa ccgacagtac acggagttaa aaaagctctc
    34381 cgcaatctcg aagcgcgggt aatccggcaa cagccgcgtg taatgctctt taacgcgcag
    34441 taaaaatgcc gcgtccgtac tttggccgtt agtaatgcag cgcagttgct ccacgaccag
    34501 accaacgtga tgatcgtaaa gatggatacg gtttttcatc gcctgctgga cagcatgcca
    34561 gtcggcctgt tcgaaacgct gctgcgcccc ggaagtcact tcgaggaatc gaccatactg
    34621 agcgtcgaag ccttgcaaaa tggtttgagc aatcagtaat tccaggccac gcggcattta
    34681 gcgccctcat caggagcaga gaattgccgg atgcggcgta aacgccttat ccagcctacg
    34741 ttcggcaacg gttgtaggca tgataagacg cgcaagcgtc gcatcaggca tcgtgcaccg
    34801 attgccggat gcggcgtaaa tgccttatcc ggcctacgtt cggcaacggc tgtaggcatg
    34861 ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat tgccggatgc ggcgtgaacg
    34921 ccttatccgg cctacagtca gcaacggttg ttgttgctta gaactgcgat tcttcagtgg
    34981 agccggtcag cgcggtgact gaagacgtgc cgccctgaat aatagtcgtc actttatcga
    35041 agtaacctgt acccacttcc tgctggtgag atacgaaggt atagccatct ttcgcggcgg
    35101 caaattccgg ctgctgcact ttctcaacgt agtgcttcat accctcgccc tgggcatagg
    35161 cgtttgccag gtcaaacatg ttgaaccaca tgctgtggat acctgccagg gtgatgaact
    35221 ggaacttgta gcccatatcc gacagctgct gctggaagct ggcaatagtt ttgtcgtcga
    35281 ggtttttctg ccagttgaac gacggcgagc agttataagc cagcagtttg cccggatatt
    35341 tcgcgtggat agcttgtgca aagcgacgcg ccagttccag atccggcgtg gaggtttcac
    35401 accagaccag gtcagcatat ggcgcatacg ccaggccacg gctgatcgct tgctcaatgc
    35461 ccgcatgagt acggaagaag ccttcactgg tacgctcgcc agtaataaat tcactgtcat
    35521 acgggtcgca atcggaggta atcagatccg ccgcatcggc atcggtacga gcaaccagca
    35581 gggttggaac gcccgtcacg tcagctgcaa gacgcgccgc gaccagtttc tgaatagctt
    35641 cctgagttgg caccaaaact ttgccgccca tgtgaccgca tttcttcact gacgccagct
    35701 gatcttcgaa gtgaactgcc gctgcaccgg cttcaatcat cgctttcatc agttcaaagg
    35761 cattcaggac accgccaaaa ccggcttccg catcggcaac gatcggcagg aagtaatcga
    35821 catagcgcgg atcgcccggc tcaatgcctg cggaccattg gatctgatcg gcacgacgga
    35881 aggtgttgtt gatccgctcc accacagctg gcaccgagtt tgccggatag agcgactgat
    35941 ccggatacat gctggccgcc aggttagcgt ccgccgctac ctgccatccc gacagataga
    36001 ctgcttcaat acccgctttc gcctgttgca gcgcctgacc gccagtcagt gcaccgaggc
    36061 tgttgatgta gccttttttc gactcgccat gcagcagacg ccacattttc gccgcgccca
    36121 gttgcgccag ggtgcattca ggattgactg aaccgcgtaa tttcaccaca tcttccgcgc
    36181 tgtatgggcg agtaatgcct tcccaacgcg gttgagtcca ctctttctgt aattcttcaa
    36241 tttgttgtgt acgggttttc atgtgcagat gctccatagt tatgtggtgg tttacgctaa
    36301 caggcggtag cctggcaggg tcaggaaatc aattaactca tcggaagtgg tgatctgttc
    36361 catcaagcgt gcggcatcgt caaaacgccc ctgggagaaa cgttcttcgc ccagttcgct
    36421 ggcaatgact ttcatctctt cacccagcat ctggcggaac aaggctttgg tcaccggttt
    36481 gccattgctc aacgtttttt gatgatggat ccactgccag attgaggtac gggaaatttc
    36541 agccgtcgcc gcatcttcca tcaggccata aatcggcacg cagccgttgc cggagatcca
    36601 tgcttcgatg tactgcactg ccacgcgaat gttggcgcgc ataccttctt cggtgcgttc
    36661 accatcacaa ggtgccagca gctgatcggc agtaatcggc gcgtcttgtt cgcgcatcac
    36721 ttcaagctga tttttacggg agccgagaat gtcgttgaat accgccattg ccgtatccgc
    36781 aaggcctggg tgagcaatcc atgtgccatc gtgaccgtta ttggcttcca gcgctttatc
    36841 cgcttttact ttgttgagca cctggttatt gcgctcttca tctttgctcg gaataaacgc
    36901 tgctatgccg cccatcgcaa aagcaccgcg tttatggcag gttttaatca acaggcgtga
    36961 gtaagcattc aggaatggtt tatccatcgt cactgcctgt ctgtctggca ggacgcgatc
    37021 gggatagttt ttcaacgttt tgatatagct gaagatgtaa tcccaacgac cgcagttcag
    37081 accaacaata tggtcacgca gcgcgtgaag gatttcatcc atctggaaca cggcgggcag
    37141 cgtttcaatc agcaacgtcg ccttgatggt gccgcgcggc agattaaagc gatcttctgc
    37201 atagctgaag acttcgctcc accaggccgc ttcctgccag gactgggttt tcggcagata
    37261 gaaataggga ccgctgccct ttgccaacag tgcctgatag ttgtggaaga aatagagcgc
    37321 aaaatcaaac aggctaccgg ggattgcctc accacgccag gtgacatgtt tttccggcaa
    37381 gtgcagaccg cgtacccgac aaatcaaaac cgctggattg ggcttgagct gataaatttt
    37441 gcctgcttca ttggtatagc tgatggtgcc gttaaccgca tcgcgcaggt taatttgccc
    37501 gtcgatcact ttgttccagt ctggtgccag tgaatcttcg aaatcggcca taaagacttt
    37561 cacattggcg ttcagtgcgt tgatcaccat cttgcgctca accgggccag ttatctcgac
    37621 tcgacgatct tgtaagtccg cgggaatacc acgaattttc cagtccgtat cgcgaatgga
    37681 agccatttcc gaaataaaat caggcaaagt tccgttatca atatcttgct gctgctgaat
    37741 gcgcgctgcc agaagtttat tgcgttgtgg cgtaaaatgc gtcaccagct cagtcagaaa
    37801 ttctaccgct tcggcagtaa gaatttgctt ctcctgctcg ccatacggcc ttatgaaagc
    37861 cagttcatcg gttgttgttg cctgttcagt catcgtgcag ctcctcgtca tggatccgaa
    37921 aacttcccca ctgtgaacga aggatcgttg tgcgcttttc gttcagcaca actaagacta
    37981 ctcatttaaa atgcaaaatc aaaaacaatt tccattttaa ttaaaaatac ttaataactt
    38041 attgataaca atatgaataa aatttattca cctcctgagg tgcgtttcgg aaataaaaaa
    38101 ggcacccgaa ggtgcctgag gtaaggtgct gaatcgctta acgatcgact atcacagaag
    38161 attaatccag cgttggattc atgtgccgta gatcgtatgg cgtgatctga tagacgtaat
    38221 agttgagcca gttggtaaac agcaaattac cgtgactacg ccagctcgct cgcggtgtat
    38281 tttgcggatc attgtgcggg aaatagttat acggtacttc cgggcctagt ccggcttcca
    38341 catcgcggaa atattcctgc gccagcgttt gcgcatcata ttcgggatgg cccgtcacaa
    38401 aggcaatgcg cttatctttg ctggcaaaca gatatgcatc cccttcttcc gtctctgcca
    38461 gaatttccag atcggtgtaa tcacgaatca acgctgccgg aaagtcagca tagcgcgaat
    38521 gtggtgccag gaatgaatca tcaaagccac gcgtcagaag cgcatgagga tggagaatat
    38581 gatgctcgta aacgccagag agtttgtcgg tgcgagtttg cttaggaatg ccgtagagga
    38641 tattgagcgc ggcctgtacc gcccagcaga caaacagcgt cgaggtgacg tgatctttcg
    38701 accactccag cacctgtttg atctgcggcc agtaagcgac atcattaaac tccaccaggc
    38761 ccagcggcgc accggttaca atcagaccgt caaagttctg ttcctgaata tcttcaaagt
    38821 tacagtagaa gttgttcaga tgctctgcgg gcgtgttgcg cgattcacgg gaatcgatgc
    38881 gcaacagctg aatatcgacc tgcaaaggtg agtttgaaag caggcgcaga aactgatttt
    38941 cagtttcaat cttcttcggc atcaggttaa ggatcagaac cttaagtgga cgaatttcct
    39001 gaccagacgc acgagaagtt gtcatcacaa agacgttttc ttcacgcaag aaattgacgg
    39061 cgggtagctc gtccggcaca cgaataggca taacctgatt acctcactac atacgcttat
    39121 acgtttagac atccagatag ctgaagataa ccagaaaatt tgccaatgtc gagcctgcat
    39181 gttgaaggtg agaaagtttc aaggaaaagg ctacgttaga atataagaat gacgaaaagg
    39241 agagaaaatg gttattagta ttcgccgctc acagcatgag gaaggggagg aactcgttgc
    39301 gatttggtgt cgatctgtcg atgccaccca cgatttttta tcagcagagt atcgggccga
    39361 gctggaagag ctggttcgtt cctttctgcc ggaagcaccg ttgtgggtcg cggttaatga
    39421 acgggatcag ccggttgggt ttatgttgtt aagtgggcag catatggatg cgctgtttat
    39481 cgatcctgat gtgcgcggct gcggcgtggg tcggatgctg gtggagcatg cgctctcgat
    39541 ggcaccggaa ctgacaacca acgttaatga gcaaaatgag caggcggttg ggttctataa
    39601 gaaggtgggt tttaaggtta cgggacgctc tgaggtggac gatttgggga aaccgtatcc
    39661 gttgctgaat ctggcgtatg tgggggaata agccgcccct gccgtgcctg cccaggctgg
    39721 agaagacgcg ccagcgtcgc acccggcatt tttttcacca acgcaaaaag gccatcgtga
    39781 ctgatggcct tt
//
LOCUS       DAZGAN020000035        39615 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGAN020000035 DAZGAN020000000
VERSION     DAZGAN020000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.035"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..95
                     /locus_tag="ACHKYO_RS10125"
                     /old_locus_tag="pgaptmp_002025"
     CDS             <1..95
                     /locus_tag="ACHKYO_RS10125"
                     /old_locus_tag="pgaptmp_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGU4428879.1"
                     /translation="ITTPTGEGVTSARTFVRLRRCVLVDAFRIV"
     gene            106..942
                     /gene="eutJ"
                     /locus_tag="ACHKYO_RS10130"
                     /old_locus_tag="pgaptmp_002026"
     CDS             106..942
                     /gene="eutJ"
                     /locus_tag="ACHKYO_RS10130"
                     /old_locus_tag="pgaptmp_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HGU4428880.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            932..2119
                     /gene="eutG"
                     /locus_tag="ACHKYO_RS10135"
                     /old_locus_tag="pgaptmp_002027"
     CDS             932..2119
                     /gene="eutG"
                     /locus_tag="ACHKYO_RS10135"
                     /old_locus_tag="pgaptmp_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HGU4428881.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDTVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            2236..3462
                     /gene="eutH"
                     /locus_tag="ACHKYO_RS10140"
                     /old_locus_tag="pgaptmp_002028"
     CDS             2236..3462
                     /gene="eutH"
                     /locus_tag="ACHKYO_RS10140"
                     /old_locus_tag="pgaptmp_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HGU4428882.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            3459..4862
                     /gene="eutA"
                     /locus_tag="ACHKYO_RS10145"
                     /old_locus_tag="pgaptmp_002029"
     CDS             3459..4862
                     /gene="eutA"
                     /locus_tag="ACHKYO_RS10145"
                     /old_locus_tag="pgaptmp_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HGU4428883.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            4874..6235
                     /gene="eutB"
                     /locus_tag="ACHKYO_RS10150"
                     /old_locus_tag="pgaptmp_002030"
     CDS             4874..6235
                     /gene="eutB"
                     /locus_tag="ACHKYO_RS10150"
                     /old_locus_tag="pgaptmp_002030"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HGU4428884.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            6256..7143
                     /gene="eutC"
                     /locus_tag="ACHKYO_RS10155"
                     /old_locus_tag="pgaptmp_002031"
     CDS             6256..7143
                     /gene="eutC"
                     /locus_tag="ACHKYO_RS10155"
                     /old_locus_tag="pgaptmp_002031"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HGU4428885.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            7153..7812
                     /gene="eutL"
                     /locus_tag="ACHKYO_RS10160"
                     /old_locus_tag="pgaptmp_002032"
     CDS             7153..7812
                     /gene="eutL"
                     /locus_tag="ACHKYO_RS10160"
                     /old_locus_tag="pgaptmp_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HGU4428886.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            7825..8325
                     /gene="eutK"
                     /locus_tag="ACHKYO_RS10165"
                     /old_locus_tag="pgaptmp_002033"
     CDS             7825..8325
                     /gene="eutK"
                     /locus_tag="ACHKYO_RS10165"
                     /old_locus_tag="pgaptmp_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HGU4428887.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKENGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            8371..9423
                     /gene="eutR"
                     /locus_tag="ACHKYO_RS10170"
                     /old_locus_tag="pgaptmp_002034"
     CDS             8371..9423
                     /gene="eutR"
                     /locus_tag="ACHKYO_RS10170"
                     /old_locus_tag="pgaptmp_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HGU4428888.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(9429..10328)
                     /gene="hemF"
                     /locus_tag="ACHKYO_RS10175"
                     /old_locus_tag="pgaptmp_002035"
     CDS             complement(9429..10328)
                     /gene="hemF"
                     /locus_tag="ACHKYO_RS10175"
                     /old_locus_tag="pgaptmp_002035"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HGU4428889.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(10332..11201)
                     /gene="amiA"
                     /locus_tag="ACHKYO_RS10180"
                     /old_locus_tag="pgaptmp_002036"
     CDS             complement(10332..11201)
                     /gene="amiA"
                     /locus_tag="ACHKYO_RS10180"
                     /old_locus_tag="pgaptmp_002036"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HGU4428890.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            11415..11840
                     /gene="ypeA"
                     /locus_tag="ACHKYO_RS10185"
                     /old_locus_tag="pgaptmp_002037"
     CDS             11415..11840
                     /gene="ypeA"
                     /locus_tag="ACHKYO_RS10185"
                     /old_locus_tag="pgaptmp_002037"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGU4428891.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            11827..12276
                     /gene="yfeZ"
                     /locus_tag="ACHKYO_RS10190"
                     /old_locus_tag="pgaptmp_002038"
     CDS             11827..12276
                     /gene="yfeZ"
                     /locus_tag="ACHKYO_RS10190"
                     /old_locus_tag="pgaptmp_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HGU4428892.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            12337..12912
                     /gene="yfeY"
                     /locus_tag="ACHKYO_RS10195"
                     /old_locus_tag="pgaptmp_002039"
     CDS             12337..12912
                     /gene="yfeY"
                     /locus_tag="ACHKYO_RS10195"
                     /old_locus_tag="pgaptmp_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HGU4428893.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            13008..13907
                     /gene="yfeX"
                     /locus_tag="ACHKYO_RS10200"
                     /old_locus_tag="pgaptmp_002040"
     CDS             13008..13907
                     /gene="yfeX"
                     /locus_tag="ACHKYO_RS10200"
                     /old_locus_tag="pgaptmp_002040"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HGU4428894.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(13965..15269)
                     /gene="pbp4b"
                     /locus_tag="ACHKYO_RS10205"
                     /old_locus_tag="pgaptmp_002041"
     CDS             complement(13965..15269)
                     /gene="pbp4b"
                     /locus_tag="ACHKYO_RS10205"
                     /old_locus_tag="pgaptmp_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HGU4428895.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(15274..16698)
                     /gene="murP"
                     /locus_tag="ACHKYO_RS10210"
                     /old_locus_tag="pgaptmp_002042"
     CDS             complement(15274..16698)
                     /gene="murP"
                     /locus_tag="ACHKYO_RS10210"
                     /old_locus_tag="pgaptmp_002042"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HGU4428896.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(16702..17598)
                     /gene="murQ"
                     /locus_tag="ACHKYO_RS10215"
                     /old_locus_tag="pgaptmp_002043"
     CDS             complement(16702..17598)
                     /gene="murQ"
                     /locus_tag="ACHKYO_RS10215"
                     /old_locus_tag="pgaptmp_002043"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HGU4428897.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            17762..18619
                     /gene="murR"
                     /locus_tag="ACHKYO_RS10220"
                     /old_locus_tag="pgaptmp_002044"
     CDS             17762..18619
                     /gene="murR"
                     /locus_tag="ACHKYO_RS10220"
                     /old_locus_tag="pgaptmp_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HGU4428898.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            18748..19539
                     /gene="ucpA"
                     /locus_tag="ACHKYO_RS10225"
                     /old_locus_tag="pgaptmp_002045"
     CDS             18748..19539
                     /gene="ucpA"
                     /locus_tag="ACHKYO_RS10225"
                     /old_locus_tag="pgaptmp_002045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HGU4428899.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            19710..20726
                     /gene="cysP"
                     /locus_tag="ACHKYO_RS10230"
                     /old_locus_tag="pgaptmp_002046"
     CDS             19710..20726
                     /gene="cysP"
                     /locus_tag="ACHKYO_RS10230"
                     /old_locus_tag="pgaptmp_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HGU4428900.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            20726..21559
                     /gene="cysT"
                     /locus_tag="ACHKYO_RS10235"
                     /old_locus_tag="pgaptmp_002047"
     CDS             20726..21559
                     /gene="cysT"
                     /locus_tag="ACHKYO_RS10235"
                     /old_locus_tag="pgaptmp_002047"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HGU4428901.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            21559..22434
                     /gene="cysW"
                     /locus_tag="ACHKYO_RS10240"
                     /old_locus_tag="pgaptmp_002048"
     CDS             21559..22434
                     /gene="cysW"
                     /locus_tag="ACHKYO_RS10240"
                     /old_locus_tag="pgaptmp_002048"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HGU4428902.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            22424..23521
                     /gene="cysA"
                     /locus_tag="ACHKYO_RS10245"
                     /old_locus_tag="pgaptmp_002049"
     CDS             22424..23521
                     /gene="cysA"
                     /locus_tag="ACHKYO_RS10245"
                     /old_locus_tag="pgaptmp_002049"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HGU4428903.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            23655..24566
                     /gene="cysM"
                     /locus_tag="ACHKYO_RS10250"
                     /old_locus_tag="pgaptmp_002050"
     CDS             23655..24566
                     /gene="cysM"
                     /locus_tag="ACHKYO_RS10250"
                     /old_locus_tag="pgaptmp_002050"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HGU4428904.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(24569..24937)
                     /gene="yfeK"
                     /locus_tag="ACHKYO_RS10255"
                     /old_locus_tag="pgaptmp_002051"
     CDS             complement(24569..24937)
                     /gene="yfeK"
                     /locus_tag="ACHKYO_RS10255"
                     /old_locus_tag="pgaptmp_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HGU4428905.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            25042..25893
                     /gene="pdxK"
                     /locus_tag="ACHKYO_RS10260"
                     /old_locus_tag="pgaptmp_002052"
                     /gene_synonym="thiJ"
     CDS             25042..25893
                     /gene="pdxK"
                     /locus_tag="ACHKYO_RS10260"
                     /old_locus_tag="pgaptmp_002052"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HGU4428906.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(25935..26444)
                     /gene="crr"
                     /locus_tag="ACHKYO_RS10265"
                     /old_locus_tag="pgaptmp_002053"
     CDS             complement(25935..26444)
                     /gene="crr"
                     /locus_tag="ACHKYO_RS10265"
                     /old_locus_tag="pgaptmp_002053"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGU4428907.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(26485..28212)
                     /gene="ptsI"
                     /locus_tag="ACHKYO_RS10270"
                     /old_locus_tag="pgaptmp_002054"
     CDS             complement(26485..28212)
                     /gene="ptsI"
                     /locus_tag="ACHKYO_RS10270"
                     /old_locus_tag="pgaptmp_002054"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HGU4428908.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(28257..28514)
                     /gene="ptsH"
                     /locus_tag="ACHKYO_RS10275"
                     /old_locus_tag="pgaptmp_002055"
     CDS             complement(28257..28514)
                     /gene="ptsH"
                     /locus_tag="ACHKYO_RS10275"
                     /old_locus_tag="pgaptmp_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HGU4428909.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(28898..29869)
                     /gene="cysK"
                     /locus_tag="ACHKYO_RS10280"
                     /old_locus_tag="pgaptmp_002056"
     CDS             complement(28898..29869)
                     /gene="cysK"
                     /locus_tag="ACHKYO_RS10280"
                     /old_locus_tag="pgaptmp_002056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGU4428910.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(30054..30815)
                     /gene="cysZ"
                     /locus_tag="ACHKYO_RS10285"
                     /old_locus_tag="pgaptmp_002057"
     CDS             complement(30054..30815)
                     /gene="cysZ"
                     /locus_tag="ACHKYO_RS10285"
                     /old_locus_tag="pgaptmp_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HGU4428911.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            31045..32031
                     /gene="zipA"
                     /locus_tag="ACHKYO_RS10290"
                     /old_locus_tag="pgaptmp_002058"
     CDS             31045..32031
                     /gene="zipA"
                     /locus_tag="ACHKYO_RS10290"
                     /old_locus_tag="pgaptmp_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HGU4428912.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVTDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            32102..34117
                     /gene="ligA"
                     /locus_tag="ACHKYO_RS10295"
                     /old_locus_tag="pgaptmp_002059"
     CDS             32102..34117
                     /gene="ligA"
                     /locus_tag="ACHKYO_RS10295"
                     /old_locus_tag="pgaptmp_002059"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGU4428913.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            34119..34337
                     /gene="ypeB"
                     /locus_tag="ACHKYO_RS10300"
                     /old_locus_tag="pgaptmp_002060"
     CDS             34119..34337
                     /gene="ypeB"
                     /locus_tag="ACHKYO_RS10300"
                     /old_locus_tag="pgaptmp_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HGU4428914.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(34334..35332)
                     /gene="yfeH"
                     /locus_tag="ACHKYO_RS10305"
                     /old_locus_tag="pgaptmp_002061"
     CDS             complement(34334..35332)
                     /gene="yfeH"
                     /locus_tag="ACHKYO_RS10305"
                     /old_locus_tag="pgaptmp_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HGU4428915.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            35422..36348
                     /gene="yfeR"
                     /locus_tag="ACHKYO_RS10310"
                     /old_locus_tag="pgaptmp_002062"
     CDS             35422..36348
                     /gene="yfeR"
                     /locus_tag="ACHKYO_RS10310"
                     /old_locus_tag="pgaptmp_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4428916.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(36387..37151)
                     /gene="yfeN"
                     /locus_tag="ACHKYO_RS10315"
                     /old_locus_tag="pgaptmp_002063"
     CDS             complement(36387..37151)
                     /gene="yfeN"
                     /locus_tag="ACHKYO_RS10315"
                     /old_locus_tag="pgaptmp_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HGU4428917.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAQFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            37400..38233
                     /gene="xapA"
                     /locus_tag="ACHKYO_RS10320"
                     /old_locus_tag="pgaptmp_002064"
     CDS             37400..38233
                     /gene="xapA"
                     /locus_tag="ACHKYO_RS10320"
                     /old_locus_tag="pgaptmp_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HGU4428918.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            38293..39549
                     /gene="xapB"
                     /locus_tag="ACHKYO_RS10325"
                     /old_locus_tag="pgaptmp_002065"
     CDS             38293..39549
                     /gene="xapB"
                     /locus_tag="ACHKYO_RS10325"
                     /old_locus_tag="pgaptmp_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HGU4428919.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKAVILMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
BASE COUNT         9239 a        10017 c        10986 g         9373 t
ORIGIN      
        1 ccatcaccac gccaaccggt gaaggggtaa ccagcgcgcg gacgtttgtc cgtctgcgtc
       61 gctgtgtatt agtcgatgcg tttcgcattg tttaaggagt aaccgatggc gcacgacgaa
      121 caatggctca ccccacgtct gcaaacggcg gcaacgctgt gtaaccagac gcccgccgcg
      181 acggaatctc cgctgtggct gggggtggat ctgggcacct gcgatgtggt gtcgatggtt
      241 gtcgaccgcg acggtcagcc ggtggcggta tgcctcgact gggccgatgt ggtccgcgac
      301 ggcatcgtct gggatttctt cggcgctgtc accattgttc gtcgccatct cgacacgctc
      361 gagcagcaat tcggtcgccg ttttagccat gcggcgactt catttcctcc aggtacggac
      421 ccgcgtattt ccatcaacgt gctggagtct gctggactgg aagtgagcca tgtgctggat
      481 gagccaaccg cagttgcgga tctgctgcaa ctggacaacg ccggtgtagt ggatatcggc
      541 ggcggcacta ccggcattgc catcgtgaaa aagggcaaag tgacgtactc ggcggatgaa
      601 gccaccggcg gacatcacat ctctctgact ctcgccggaa atcgccgtat ttcgctggaa
      661 gaggcggagc agtacaagcg cggtcacggt gaagagattt ggcctgcggt gaaaccggtg
      721 tacgaaaaaa tggcggacat tgtcgcccgg catattgaag ggcagggcat tactgattta
      781 tggctggcgg gcggctcctg tatgcaaccg ggcgtggcgg aactgtttcg caaacaattc
      841 ccggcgttac aagtgcattt accgcagcac agtttgttta tgaccccgct ggcgatcgcc
      901 agtagtgggc gagagaaagc ggaggggcta tatgcaaaat gaattgcaga ccgcgctctt
      961 tcaggcgttc gataccctga atctgcaacg ggtaaaaaca tttagcgttc caccggtgac
     1021 gctttgcggt ccgggctcgg tgagcagttg cggacagcaa gcgcaaacgc gtgggctgaa
     1081 acatctgttc gtgatggcag acagcttttt gcatcaggca gggatgaccg ccgggctgac
     1141 gcgcagcctg gctgttaaag gcatcgccat gacgctctgg ccatgtccgg tgggcgaacc
     1201 gtgcatcacc gacgtgtgtg cagccgtggc gcagttgcgt gagtcaggct gtgatggggt
     1261 gatcgcattt ggcggcggct cggtgctgga tgcggcgaaa gccgtggcgt tgctggtgac
     1321 gaacccggat agcacgctgg cggaaatgtc agaaaccagc gttctgcaac cgcgcctgcc
     1381 gttgattgcc attccaacta ccgccggaac cggctctgaa accaccaacg taacggtgat
     1441 tatcgacacg gtgagcgggc gcaagcaggt gttagcccat gcctcgctga tgccggatgt
     1501 ggcgatcctc gacgccgcgc tgaccgaagg tgtgccgtcg catgtcacgg cgatgactgg
     1561 cattgatgcg ttaacccatg ccattgaagc gtacagcgcg ctgaacgcca caccgtttac
     1621 cgacagtctg gcgattggtg ccattgcgat gattggcaaa tcgctgccga aagcggtggg
     1681 ctacggtcat gaccttgccg cgcgcgagag catgttactg gcttcatgta tggcgggaat
     1741 ggcgttttcc agtgcggggc ttgggttgtg ccacgcgatg gcgcatcagc caggggcggc
     1801 gctgcatatt ccacacggtc tggcgaacgc catgttgctg ccaacggtga tggaatttaa
     1861 ccggatggtt tgtcgcgaac ggtttagtca gattggtcgg gcactgcgaa ctaaaaaatc
     1921 cgacgatcgt gacgctatta acgcggtaag tgagctgatt gcggaagttg ggattggtaa
     1981 acgactgggc gatgttggcg cgacatctgc gcattacggt gcatgggcgc aggccgcgca
     2041 ggaagatatt tgtctgcgca gcaacccgcg taccgccagc ctggagcaga ttgtcggcct
     2101 gtacgcagcg gcgcaataaa tgccggatgc gacgcttgcc gtgtctgatc cggccttcgg
     2161 gattgcacat gtagggcgga taaggcgttt acgccgcatc cgccaataaa taacaactaa
     2221 cagggagtaa aggcgatggg aattaacgaa atcatcatgt acatcatgat gttctttatg
     2281 ctgatagctg ccgtagacag gatcctgtcg cagttcggcg gttctgctcg tttcctcggt
     2341 aagttcggta aaagtatcga aggatcaggc ggtcagttcg aagaaggctt tatggcaatg
     2401 ggcgcactgg gcctggcgat ggtcggtatg accgcgctgg caccggtact ggctcacgta
     2461 ctcgggccgg taattattcc ggtttacgaa atgctcggcg ctaacccatc gatgttcgcc
     2521 ggaacactgc tggcgtgcga tatgggcggc ttcttcctcg ccaaagagct ggcgggcggc
     2581 gacgtagcag cgtggctata ctctgggtta attctcgggt cgatgatggg gccaacgatt
     2641 gtgttttcca ttccggtggc gctcggcatt atcgaacctt ctgaccgtcg ttatctggcg
     2701 ctcggcgtgc tggcgggcat tgtgaccatt ccgattggct gtattgctgg tggtctggtt
     2761 gctatgtact ccggtgtgca gatcaacggt cagccggtgg aattcacttt cgccctgatc
     2821 ctgatgaaca tgatcccggt gatcattgtc gcgattctgg tggcgctggg gctgaaattc
     2881 atcccggaaa aaatgatcaa cggcttccag atcttcgcca aattcctcgt tgcattgatc
     2941 accctcggtc ttgccgctgc ggtagtgaaa ttcctccttg gctgggaact gatccccgga
     3001 ctggatccta tctttatggc ccctggcgat aaacccggtg aagtgatgcg cgccattgaa
     3061 gttatcggtt ctatctcctg cgtgctgtta ggggcgtatc caatggtgct gctgctgact
     3121 cgctggtttg aaaaaccgct gatgagcgtc ggtaaagtgc tgaatatgaa caacatcgcg
     3181 gcagccggca tggtggcaac gcttgccaac aacatcccga tgttcggcat gatgaagcag
     3241 atggataccc gcggcaaagt catcaactgc gccttcgccg tttccgctgc tttcgccctg
     3301 ggcgaccact taggcttcgc cgctgccaac atgaacgcca tgatcttccc gatgattgtc
     3361 ggcaagttga tcggcggcgt aacggcgatt ggcgtggcga tgatgctggt gccaaaagaa
     3421 gacgcgaccg cgactaaaac cgaagcggag gcacaatcgt gaacactcgc cagctattga
     3481 gcgtcggtat cgatatcggc accaccacca cccaggtgat tttctcccac ctggagctgg
     3541 ttaaccgtgc ggcggtgtcg caggtgccgc gctacgaatt cattaaacgc gaaattagct
     3601 ggcaaagtcc ggtgttcttt acccctgtcg ataaacaggg cggtttaaaa gaagcggaac
     3661 tgaaaacctt aatactcgag caatatcagg ctgcgggtat tgcgccggaa agcgttgatt
     3721 ctggtgccat catcatcacc ggtgaaagcg cgaaaacccg caatgctcgc ccggcggtga
     3781 tggcgctctc tcaatcgctg ggggattttg tcgttgccag cgccgggccg cacctcgaat
     3841 ccgtgatcgc cggtcacgga gctggggcgc aaaccctttc tgaacaacgg ctgtgtcggg
     3901 tactgaatat cgacatcggc ggtggcaccg cgaactacgc cctgttcgat gccggaaaaa
     3961 tcagcggcac tgcctgtctc aacgtcggtg gtcgcctgct ggaaaccgac agccaggggc
     4021 gcgtggttta cgctcataaa ccggggcaga tgattgtgga tgagtgcttc ggtgcaggca
     4081 ctgacgcccg ttcgctgacc ggcgcgcagc tggtgcaggt tacccggcgg atggcggcgc
     4141 tgattgtcga agtgattgac ggaacgcttt cgccgctcgc gcaggcattg atgcaaaccg
     4201 gtttgctgcc cgcaggtgtt acgcccgaaa tcattacgct ttctggaggc gtgggcgaat
     4261 gttatcgcca ccagcccgcc gacccgttct gttttgccga tattggccca cttctggcaa
     4321 cggcgctgca tgaccatccg cgcctgcgtg agatgaatgt gcagtttccg gcgcaaaccg
     4381 tacgcgccac ggtgattggc gcgggtgcac ataccctttc gctctctggc agcacaatct
     4441 ggctggaggg cgtacaactg ccgctgcgca atttgccggt ggcgatcccg attgatgaaa
     4501 cggatctggt gagtgcctgg caacaggcgc tgcttcagct ggatcttgat cccaaaactg
     4561 acgcgtacgt gctggcgctt cccgcctcgc tgcctgtgcg ttacgccgcg gtactgacgg
     4621 tcatcaacgc gctggtcgat ttcgtcgcgc gttttccgaa tccgcatccc ctgctggtgg
     4681 tggccgggca ggactttggt aaagctctgg gcatgttgtt gcgcccacag ctacaacaac
     4741 tcccgttggc agtcattgac gaagtgattg tccgcgcggg ggactatatc gacattggta
     4801 cgcctctttt tggcggatcg gttgtgccgg tgacggtgaa atcactcgca tttccttcct
     4861 gagggaacga cttatgaaac taaagaccac attgttcggc aatgtatatc agtttaagga
     4921 tgtaaaagag gtgctggcta aagccaacga actgcgttcg ggggatgtgc tggcgggcgt
     4981 tgcagcggca agctcacagg agcgcgtggc ggcaaagcag gtgttgtcgg aaatgaccgt
     5041 agcggacatc cgcaataatc cggtgattgc ctatgaagat gactgcgtga cgcggctgat
     5101 tcaggacgat gttaacgaaa cggcctacaa ccagattaaa aactggagca tcagcgaact
     5161 gcgtgagtat gtgctgagcg atgaaaccag cgtggacgac attgccttta cccgcaaagg
     5221 gctgacctcg gaagtggtcg cggcggtagc gaagatttgc tccaacgcgg acctgatcta
     5281 cggcgcgaag aaaatgccgg taatcaaaaa ggccaatacc accattggta ttccgggcac
     5341 cttcagcgcc cgtttgcagc caaatgacac ccgtgacgac gtgcaaagta tcgccgcgca
     5401 aatctacgaa gggctttcct tcggggtggg cgatgcggtg atcggcgtta acccggtgac
     5461 tgacgacgtg gaaaacttaa gccgcgtgtt ggacaccatt tacggcgtga tcgacaaatt
     5521 caacatccca actcagggct gcgtactggc gcacgtcacc acccagatcg aagcgattcg
     5581 tcgcggcgcg cctggcggac tgattttcca gagtatctgt ggcagcgaaa aagggctgaa
     5641 agagtttggc gtggaactgg cgatgctcga cgaagcgcgc gcagtgggtg cagagttcaa
     5701 ccgtatcgcc ggggaaaact gcctctactt cgaaaccgga caaggctctg cgctatccgc
     5761 tggcgctaac ttcggcgctg accaggtgac gatggaagca cgtaactatg ggctggcgcg
     5821 tcattacgat ccgtttatcg tcaacaccgt ggtcggcttt attgggccgg agtatctcta
     5881 caacgaccgc cagattatcc gtgctggctt agaagatcac tttatgggca agctgagcgg
     5941 catctctatg ggctgtgact gctgctacac caaccacgct gacgctgacc agaacctcaa
     6001 cgaaaacctg atgatcctgc tcgccaccgc aggctgcaac tacatcatgg ggatgccgct
     6061 gggtgatgac atcatgctca actaccagac caccgcattc cacgataccg ccactgtgcg
     6121 tcagttactc aacctgcgcc cgtcaccaga gtttgaacgc tggctggaaa gcatgggcat
     6181 tatggcaaac ggtcgcctga ccaaacgggc gggcgatccg tcactgttct tctgatgacg
     6241 cggagataac acatcatgga tcaaaaacag attgaagaaa ttgtacgcag cgtgatggcg
     6301 tcaatgggac aaacggcccc cgcgccgtca gaagcaaagt gcgccaccac caactgtgcg
     6361 gcaccggtga cctcggaaag ctgtgcgctg gatttaggtt ccgctgaagc aaaagcgtgg
     6421 attggtgttg aaaacccgca tcgcgcagac gtattaacag aactgcgccg cagcaccgtg
     6481 gcccgcgttt gtaccggtcg tgccggtccg cgtccacgta cccaggcgct gctgcgtttc
     6541 ctggccgatc actctcgttc gaaagatacc gtactgaaag aagtgccgga agagtgggtg
     6601 aaagcgcagg gcttgctgga agtacgctct gaaattagcg acaaaaatct ctacctgact
     6661 cgcccggata tgggccgccg cctgtgtgca gaagctgttg aagcgctgaa agcacagtgt
     6721 gttgctaatc cggacgtaca ggtcgttatt tctgatggcc tgtcaactga tgccatcacc
     6781 gtgaactacg aagagatcct gccgccgctg atggcgggcc tgaaacaggc cgggctgaaa
     6841 gtcggcacgc cattctttgt tcgttatggt cgcgtgaaga ttgaagatca gatcggtgag
     6901 atcctcggcg cgaaagtggt gatcctgctg gtgggggaac gtccggggtt agggcagtca
     6961 gaaagcctct cctgctacgc cgtttactcg ccgcgtatgg cgaccaccgt tgaggccgat
     7021 cgcacctgta tctctaacat tcaccagggc ggcacgccgc cggttgaagc cgcagccgtc
     7081 attgtggatt tggcgaaacg tatgctggag cagaaagcat ccggcatcaa catgacccga
     7141 taaggaggca tcatgccagc tttagatttg attcgaccgt cggtaaccgc catgcgggtg
     7201 attgcctccg ttaacgctga atttgcgcgt gaactaaaat tgccgccgca tattcgtagc
     7261 ctcgggctga tttctgctga ttccgatgac gtcacctaca ttgccgctga tgaagcgacc
     7321 aagcaggcga tggtggaagt ggtatatggt cgctcgctgt atgcgggcgc tgcacacggc
     7381 ccgtcaccga ctgccggtga agtgctgatt atgcttggtg ggccaaaccc ggcggaagtg
     7441 cgtgccggtc tggatgcgat ggttgcacat attgaaaatg gtgcagcatt ccagtgggcc
     7501 aatgacgcag aaaacaccgc cttcctggcg catgtggttt cgcgtaccgg ttcttatctc
     7561 tcatcaaccg ccgggatcac gcttggcgat ccgatggcgt atctggtggc accgccgctg
     7621 gaagcgacct atggcattga tgcagcgttg aaatctgccg acgtacagct ggtgacctat
     7681 gtcccgccac cgtctgaaac caactactcg gcagcatttt taaccggtag ccaggccgcg
     7741 tgtaaagcag cctgtaacgc ctttaccgat gcagtgctgg aaatcgcgcg taatccaatc
     7801 cagcgtgcgt aacggaggtt gccgatgatc aatgcactgg gattgctgga agtggacggc
     7861 atggtcgccg cgatagatgc agcggatgcc atgctcaaag cagctaacgt tcgtctgctc
     7921 agtcacgaag tgcttgaccc tggtcgctta acgctggtgg tggaaggcga tctggcggcg
     7981 tgtcgtgcgg cgctggacgc aggttgtgct gccgcgatgc gtaccgggcg tgtcatcagc
     8041 cgcaaggaga acggtcggcc agacgatgac acccagtggc tggttactgg ctttaaccgc
     8101 cagccgaagc aacccgtaag ggaacccgac gcgccagtta tcgtcgcgga atctgctgac
     8161 gagttgttgg cgctgttaac atcagtacgt cagggaatga cggcaggaga agtggctgcc
     8221 cactttggct ggccgctgga aaaagccaga aatgcgctcg aacagctctt ttctgccggg
     8281 acgttacgta aacgcagtag tcgttatcgt ctcaagcccc attaacctgt cggaggtgcc
     8341 gggtgtccta caacacccgg cattaacatc atgaaaaaga cccgtacagc caatttgcac
     8401 catctttatc atgaaccctt acccgaaaac ctgaagctca cgccgaaggt cgaagtggat
     8461 aatgttcatc aacgacagac aacggatgtc tatgaacatg ctttaacgat taccgcctgg
     8521 cagcagattt acgatcagct gcatccgggc aagtttcatg gtgaatttac ggaaattcta
     8581 ctcgatgata ttcaggtttt tcgtgaatac accggtctgg cgctgcgtca gtcgtgcctg
     8641 gtctggccga actcgttctg gtttggcatt ccggcgacgc gcggtgagca gggatttatc
     8701 ggttcgcaat gtctgggaag cgcggaaatc gccacccgcc ctggtggcac tgaatttgaa
     8761 ctgagcacgc cggatgatta cacgatcctg ggcgtggtgc tttctgaaga tgtcatcacc
     8821 cggcaggcta actttttgca taacccggat cgggtattac atatgctgcg tagccagtcg
     8881 gcgctggaag tgaaagagca gcataaagcc gcgctgtggg gctttgtcca acaggcgctg
     8941 gcgacgtttt gcgagaaccc ggaaaatctc catcagcctg cagtgcgaaa agtgctgggg
     9001 gataatttgc taatggcgat gggggcgatg ctggaagacg cgcaaccaat ggtgacggcg
     9061 gaaagcatca gtcatcagag ttaccgtaga ttactttccc gcgcccgtga atatgtgctg
     9121 gaaaatatgt ctgagccggt gacggtgctg gacttgtgta atcaactgca tgtcagtcgc
     9181 cgcacgctac aaaacgcgtt tcacgctatt ttaggcattg gcccaaacgc gtggctgaaa
     9241 cgcattcgcc tgaacgccgt acgccgcgaa ctgataagcc cgtggtcgca aagcacaacg
     9301 gtaaaagacg ccgccatgca gtggggattc tggcatctgg ggcaatttgc cacggattac
     9361 cagcagctgt ttgccgagaa gccgtcgttg acgttgcatc agcggatgcg ggaatggggg
     9421 tgagggagtt acacccaatc cctgacctta ataaactcac ttaacgccgc ttctgggctg
     9481 ccatcttttg gctgataatc atactcccag cgtaccagtg gcggcattga catcaggatc
     9541 gactcggtgc gcccgccagt ttgcaggcca aacagagtgc cgcgatccca gaccagattg
     9601 aactcgacat aacgaccgcg acggtagagc tgaaaattgc gctcgcgctc gccgtaggcc
     9661 atcgctttac gtcgctctac aattggtaaa taagcgtcgg tgtagccttt gcctaccgcc
     9721 tgcataaagg caaaacagtg gtcgaaatct ggcgtgttca gatcatcaaa gaacagcccg
     9781 ccaataccgc gctgttcgtt gcgatgtttg aggtagaagt aatcgtcgca ccactttttg
     9841 taacggggat aaacgtcttc accaaatggc aggcacaggt cacgggcggt gcggtgccag
     9901 tgaatggcgt cttcttcaaa accatagaaa ggggttaaat cgaagccgcc gccaaaccac
     9961 cagacgggct ctgcacccgg tttttcggca ataaaaaaac gcacattcgc gtggctggtg
    10021 ggaacatacg ggttatgcgg atgcactacc agtgaaacgc ccatcgcctc gaaactgcgc
    10081 ccggcaagtt ccgggcgatg agcggtggcg gaagctggca tcgcctcgcc gtggacgtga
    10141 gaaaagttga cgcctgcctg ttcgaaaaca ccaccattac gcaacacccg actacgcccg
    10201 ccgccgccag cttcgcgctg ccaactatct tcgacaaatt ctgcgccatc gacggcggtc
    10261 agctgctgac aaatcgtatc ctgaaggttg agcagaaact gtttaacctg gtgtgcgtcg
    10321 ggtttcataa cttatcgctt tttcgaatgt gctttctggt tgtcgaacca gtggaaataa
    10381 ctgatcacgc cttcagcaat cgctgtggcg attttctgac gaaacgccgc cgtgcctaac
    10441 agccgctctt cttccgggtt ggtgataaac gaggtttcca ccagcaccga aggaaccgac
    10501 ggcgatttca acaccacaaa cgcggcttgt tcggtgttgc ggctgtgcag tttatgcacc
    10561 ggcttaatct ttttcagaat atgcgagccg agcgtcaggc tgtttttaat agtatcggtt
    10621 tgcaccagat caaacagcac ttgctgcaat aggtgatcct tgtcagtcgc ctttttaccg
    10681 gcaacttcat cggcgcggtt ttcgcgttca gacaggtatt tcgccattgc gctacttgcc
    10741 ccacggttag agagggcaaa taccgaagca ccggcagctt tcgggttggt aaagccatcg
    10801 gcatgaattg acataaacag atctgcgcca tgtttatggg cgatttcaac gcgatcgtaa
    10861 agtgggataa acgtatcgcc agaacgcgtt aaacgcgcat caatcccatg attacgcaaa
    10921 atggaacgga cgtttttagc aatcgccagc accacatgtt tttctttcga accgttgcgt
    10981 ccgatcgctc cggtatcaat tccgccgtgg cctggatcga gaacaacgac acgtttgccg
    11041 ccagattttt tggctttcgg cttgctgtgt ccgttgctgg tttttaaagg ttcgtctttg
    11101 gcgatggctt gcgacattcc tgacaacgtc agggcagcca aaccggcttt cagcacctgg
    11161 cggcgcgaag tgagtgtttt tagtggttta aaagtgctca tacggcctga gttgtaataa
    11221 taaagttcca gatgttatat cgtatcgcgt aatccgttac gacggtttga tgttgagaat
    11281 tgttttactt ttcatttcaa tagttgacaa ctccccatta tgccactttt tcaaccgatt
    11341 ttcgtcagat attggctaat tcgaccgttc acgccataat cagcctttta acccgcaaaa
    11401 aggtaacgaa taccatggag atacgcgtat ttcgccagga agatttcgaa gaggtcatca
    11461 ccctttggga gcgttgcgac ttgctgcgtc cgtggaacga tccggaaatg gacatcgagc
    11521 gtaagatgaa ccatgacgtc agtttgtttt tggtcgctga ggtaaacggt gaagtggtcg
    11581 gaacggtgat gggcggttat gacgggcatc gcgggtctgc ttattatctt ggcgtgcatc
    11641 cagagtttcg tgggcgtggg attgccaatg cgttgcttaa tcggctggag aaaaagctga
    11701 ttgctcgtgg ctgcccgaaa attcagatca atgtgccgga agataacgac atggtgctcg
    11761 gaatgtatga acgcctggga tatgaacacg ccgacgtgct gagtctgggt aagcgtttga
    11821 ttgaagatga agagtactga gttccatcct gtccattatg atgcgcacgg acgcctgcgt
    11881 ttacccttgc tcttctggct tgtgctattg cttcaggcgc gaacctgggt gctgtttgtc
    11941 attgccggtg cgtcgcgcga gcagggtact gcgctgctaa atctgtttta tcccgatcac
    12001 gataatttct ggctggggct gattccaggc attccggcgg tgctggcgtt tttgttgagc
    12061 ggtcggcggg ctacatttcc tcgcacttgg catgtgctct attttctgtt gctgttggcg
    12121 caggtggttt tactttgctg gcaaccgtgg ctgtggctga acggtgaatc cgttagcggt
    12181 atcggtctgg cgctggtagt ggcggatatt gtggcgttaa tctggctgct gaccaatcga
    12241 cgtttacgcg cttgttttaa tgaagaaaaa gaataacggc actttttggt gaatttgcac
    12301 tccaagcaac gttattgaat aaccaaagga agtgacatga aatcgctgcg tttaatgtta
    12361 tgcgctatgc cgttgatgct gaccggctgt tccacgatgt cgtcagttaa ctggtcggcc
    12421 gctaacccgt ggaactggtt tgggtcgtcc accaaagtga gcgagcaggg cgttggtgaa
    12481 ttaacggcgt ccacaccact gcaagaacaa gccattgccg atgcgcttga tggcgattat
    12541 cgcctgcgca gcggaatgaa aaccgcgaac ggcaacgtgg tgcgcttttt tgaagtgatg
    12601 aagggcgaca acgtggcgat ggtgattaac ggcgatcaag gcacgatcag tcgcattgac
    12661 gtgctggata gcgatattcc tgctgacacc ggtgttaaaa tcggtacacc gtttagcgac
    12721 ctttacagca aagcatttgg caattgccaa aaagctgatg gtgatgataa tcgtgccgtc
    12781 gaatgtaaag ccgaaggcag tcaacatatt agctaccagt tcagcgggga atggagtggt
    12841 cctgaagggt taatgccttc ggacgatacc ctgaaaaact ggaaagtcag taaaattatc
    12901 tggcggcgtt aatttgcgtc tgaacaaacc gccgccgcga aaaaacacgt aaaatagcgc
    12961 tcaacaatgc cacggattgc gtggcattat tattttcagg aggaacaatg tctcaggttc
    13021 agagtggcat tttgccagaa cattgccgcg cggcgatttg gatcgaagcc aacgtgaaag
    13081 gggaagttga cgccctgcgt gcggccagta aaacatttgc cgacaaactg gcaacttttg
    13141 aagcgaaatt cccggacgcg catcttggtg cggtggttgc ctttggtaac aacacctggc
    13201 gcgctctgag cggcggcgtt ggggcagaag agctgaaaga ttttccgggc tacggtaaag
    13261 gccttgcgcc gaccacccag ttcgatgtgt tgatccacat tctttctctg cgtcacgacg
    13321 taaacttctc tgtcgcccag gcggcgatgg aagcctttgg tgactgcatt gaagtgaaag
    13381 aagagatcca cggcttccgt tgggttgaag agcgtgacct gagcggcttt gttgacggta
    13441 cggaaaaccc ggcgggtgaa gagacgcgtc gcgaagtggc ggttatcaaa gacggcgtgg
    13501 atgcgggcgg cagctatgtg tttgtccagc gttgggaaca caacctgaag cagctcaacc
    13561 ggatgagcgt tcacgatcag gagatgatga tcgggcgcac caaagaggcc aacgaagaga
    13621 tcgacggcga cgaacgtccg gaaacctctc acctcacccg cgttgatctg aaagaagatg
    13681 gcaaagggct gaagattgtt cgccagagcc tgccgtacgg cactgccagt ggcactcacg
    13741 gtctgtactt ctgcgcctac tgcgcgcgtc tgcataacat tgagcagcaa ctgctgagca
    13801 tgtttggcga taccgatggt aagcgtgatg cgatgttgcg tttcaccaaa ccggtaaccg
    13861 gcggctatta tttcgcaccg tcgctggaca agttgatggc gctgtaagcg tttctgattc
    13921 attgattaaa ggccaggcag taacattact gactggcctt tttattactt ttgctttaac
    13981 gccgcataca cctgatcaac tacccaacca tacgttgcaa tcggcagctg accgctttcg
    14041 aacatattgg gattcttttg cggatcggca acaggcgaat gtggcttgtt gcttaacatc
    14101 acaatcgcca tatgattcac cggatcgata acggtcaccg ttccggtcca gccagtgtga
    14161 ccgtaggttt gcgggcttgc cagcgtgcca aacgtcggcg tcatggtggc attaccattc
    14221 acgcgccagc cgaggccaaa agtggcatct tccttagagc tggtggtgaa catcttcacc
    14281 gtttccgcac tgaacagctg cacatcgcca tagcccccgc cgttcagcat cgtttgcatt
    14341 aacaccgcaa tatcgccggt attggaaaac aaacctgcgt gcccggaaac gccgcccatc
    14401 gaataaaagg ctttttcatc gtgcacctga ccccagagag tggaggtgcg gatattcggg
    14461 aaatggatca cgccatcgcg ggtattgccg ttcaattccg tggcggcaat ttgttgcggt
    14521 ttaaagcctt tcagcagcgg gttaaatacc gtatgtgtca ggccgagcgg gcgataaatc
    14581 gactcttcaa catagcggtc aagcggctga ccggtgaccg actcgacgat aaatccaagc
    14641 agcatataat cgacatcact gtaaatatgc ttgctgccgg gctgatactc cagcggcgtg
    14701 cgcttgatca tctccagcgt ctggccttta tcctgggaat ataacgcgcc cgcgaccgct
    14761 ttattcggat attgtgggtc cgccgggaaa ccgccgctgt gatgcagcag gtcagaaatc
    14821 cgcagcgtgt ttttgccttt gatggtgtca tttggactat cggcaaaccc cggaatatat
    14881 ttcgcaatcc gatcgtcagg atgcagtttg ccttcgctca tcagcttttg cagggcgaag
    14941 ttcgtggcgt acattttggt gtttgaggcc aggtcataca gcgtcccggt ggtggctttg
    15001 acaggctgtt ccatcaacac gctgccatcg tactttttcg ccgctcccca ggctttgcga
    15061 tacacaatct gattatcttt aatgatcagc aggtttacgc cgggataacc ggcgtccact
    15121 tgctggctaa tccagcgatc catctggtta agccgttcga cgttgaaccc tgctttctct
    15181 ggcgaacttt ctgtcagaac agggtatttt gcggcgctga cgctacaact aacagccagc
    15241 agagaaagat agagcattgt ccgtttcatg atatcagtcc agattgacgt tacggcagcc
    15301 aaagagcgtg gtgaaaataa acccgcaaac ccatgccacc agaatcccgc cagcataaac
    15361 cgccattgcc ggaaggatgc cttgtgcgga agtcatcagc ggcagcgcca ccagaccaga
    15421 cggcccaaag gcgctgttta agcccatcgg cagaccccac caggctatca ggccgataaa
    15481 caaaccgccc gctgcgccgc ctaaacaggc ggtaataaac ggtttcatgc ggggcagggt
    15541 gacgccgtaa atcaacggtt caccaacgcc cagcagaccg ggaataatcg ccccgcgtac
    15601 ctgactgcgt aatgcactgt gcggttgcgc ccgccagtag agtgccagcg ccgcgcccac
    15661 ctggcccgcg cctgccattg aaaggatggg aaataagctg ttgaatccct ggctgtccat
    15721 taacgcgagg taaacaggaa taaagccctg atgcacgcca aataccacgg cgatcaggaa
    15781 cagcccggct aaaaccgcac aaccgaacgg attactgttc aggtgcataa acagccatga
    15841 cataccttcg aataaccagc cacccagcgg cataatgatc aggtacgcaa gcgtagcggt
    15901 gatcagcagg gtgattaacg aggtcagcag catgtcgaga tcgtccggca taaagcggcg
    15961 caccatgcct tcaatgcggg cgcaggccca cgcggcaatc agcacgccga taatattgcc
    16021 gcgcggatcg atgggcagac caaagaaatc gtgaaaaccg gcgtagtaac cggtggttgc
    16081 agcggggtta taaccgagca aaaagagtgc ggcaataatt gcgccattga cgcccgtgcc
    16141 gccaaatgcc tgggcggcgt tatagcccac cagaatcacc aggaaagtga acaaaccttt
    16201 gctgaacacc ttcataaaat tcagcgcgtc gggtagcgtg ccctgagcat ctgccggaac
    16261 gtgcatcacc gtggcaatta acgtcgctat ccccagcagc agaccggcgg caataaaacc
    16321 ggggatcagc ggcgtgaaga tggtggcgaa tttggcgaga aattgttgca cgccggaggt
    16381 ttgtttggct tttaactgac gtttgttctg ggcggcgatt tctgcggcat cctgtaccgg
    16441 tgcctcgccc agcagctcgc tcatcgcttt tgcggcacga tgcgctttac caggcccaaa
    16501 aacgacctgc acctggtcgc tggtcaaaat gacgcccttc acgccttcca gcgttttgat
    16561 attgggatca accagtgaac tgtcatgtac acccagacgc aggcgcgtca tacagttacc
    16621 acaactggcg atatttcccg gtccgccgac acgggtaaga atggtgttca gaagttcact
    16681 gctgatctct ttggccatgg gttattcctt gtctaaaacc tgacgaataa agccgccgtg
    16741 ttgatccagg cgttttttag cttctgcggc atcgagattt ttcagcacca tcacaatggc
    16801 cgttttacag ttgcgctcgc aggcaattaa cgccgcttcc gcttgttcgg cattacatcc
    16861 ggtggcgttt ttaacgatat tgacctggcg cacatgcagt ttttcgttgg tggcgaccac
    16921 atcgaccatc aggttgccga acactttgcc agatttaatc atcagcccgg tggaaagcat
    16981 attgagcacc agtttctgcg ctgtgcctgc tttcattcgc gaagaaccag tgaccacttc
    17041 ggcacctaca atcggtgtaa tggcaaactc ggcggtggtg gaaacggcac tccccggatt
    17101 acaggaaatt cccactgtgc ggcagccgag ctggcgtgcg tattccagtc cggcaatcac
    17161 atacggcgtg cgaccgctgg cagcaatgcc aaccaccaca tcctgtgccg ttaaattaat
    17221 atttttcaga tcgttaacac caccttcccg gctatcttca gcgccttcca ccgcgtgctg
    17281 aatggcatat tcgcctccag caatcaaacc aaccaccaga cccggtttca cgccgtaggt
    17341 gggcggacat tcgctggcat ccagaatccc cagacgaccg gatgttcccg caccgagata
    17401 aatcagacga ccgccgccgc tgacctgggc gtggataaca tcgatcgccg cggcgatatc
    17461 cggcaggacg cgctcaacgg caagcggtac ggttttatct tcatcgttga taatccggca
    17521 catttccagc gtcgatacgc ggtcaatttc agccgaggcg gtgttcgagc cttcagtaat
    17581 catcttttct agttgcatcg gtggtgacct tactatttac gatcaaataa ttgcagatgt
    17641 atggaatcat atatcccgaa ttgacagact taaaggaatc attgagtcta cactcgcaga
    17701 gtgtgaagag cgtcataaga acttaagcgg aatttatgct gccagcccag gagagtggaa
    17761 gatgttgtac ctgacgaaaa taagcaatgc ggggagtgaa ttcaccgaaa acgagcaaaa
    17821 aatcgctgac tttctgcagg ccaacgtgag tgagctgcaa tccgtctctt cgcgcaagat
    17881 ggcgaaacag ctcggcatca gccagtcgag tattgtgaag tttgcccaga agctcggcgc
    17941 acaaggattt actgaattgc gtatggcgtt aattggcgaa tacagtgcca gccgggagaa
    18001 aacgaatgcc acggcgctgc acctgcacag ttcgattacc agtgatgatt cgctggaagt
    18061 gattgcccgc aaactgaatc gcgaaaaaga gctggcgctg gagcaaacct gcgcgttgtt
    18121 cgactacgcg cggctgcaaa aaatcattga tgtcatcagt aaagcgcaat ttattcagat
    18181 aacgggcctg ggcggatcgg cgctggtggg gcgcgatctg tcgttcaaac tgatgaaaat
    18241 tggatatcgc gtggcctgcg aagccgacac gcacgtacaa gccaccgttt cccaggcgtt
    18301 gaaaaaaggc gatgtacaaa tcgctatctc ttacagcggc agtaaaaaag agattgtctt
    18361 atgcgcagaa gcggcacgaa agcagggggc aacggtgatt gccattactt ctctggctga
    18421 ttctccgctg cggcggctgg cgcatttcac gctcgatacc gtttccggtg aaaccgaatg
    18481 gcgtagctcg tcgatgtcta cccgcacggc gcaaaattct gttaccgatt tactgtttgt
    18541 tggtcttgtg caactcaatg atgtggagtc attgaaaatg attcagcgca gcagtgagct
    18601 aacacaacgc ctgaaatgat gcataaagca gcgactggat tgagattttc ctgaattagt
    18661 gagctgatcc gcagcaatat tttgtttatc ctgtattttc agagggaatg gagtgtaacg
    18721 ctctgtatta acaaggagag cattaaaatg ggtaaactca cgggcaagac agcactgatt
    18781 acgggcgcat tgcagggaat tggcgaagga attgccagaa cttttgcgcg tcatggcgca
    18841 aacctaatct tgctggatat ctcccctgag atcgaaaagc tggcggacga actgtgtggt
    18901 cgtggtcatc gctgtacggc ggttgtcgcc gatgtgcgtg acccggcgtc ggtagccgca
    18961 gctatcaaac gcgcgaagga aaaagaaggg cgcattgata tcctggtgaa taacgcaggc
    19021 gtttgtcgtc tgggcagttt cctcgatatg agcgatgacg atcgcgattt ccatattgac
    19081 atcaatatta aaggcgtatg gaacgtcacg aaggcggtgc tgccggagat gattgcccgc
    19141 aaagatggtc gcattgtgat gatgtcttcg gtcactggcg atatggtggc cgatcctggc
    19201 gaaacggcgt acgccttaac gaaagcggcg attgttggcc tgacaaaatc gctggcggtg
    19261 gagtacgcgc agtctggtat tcgcgttaac gccatttgcc cgggatacgt gcgcacacca
    19321 atggcggaaa gcattgcccg ccagtcgaac ccggaagatc cagagtcggt gctgactgaa
    19381 atggcgaaag caatcccgat gcgtcgcctc gccgatccgc tggaagtcgg cgaactggcg
    19441 gccttcctcg catcggatga atccagctat ttaaccggta cacagaatgt gattgatggc
    19501 ggcagcacac tgccggagac ggttagcgtc ggtatctgat tcacctctgt ttcctccctg
    19561 catgtgtggg gaggatttcg ttttaaaccc ggttcttatt cccttttcaa cttccaaatc
    19621 accaaacggt atataaaacc gttactcctt tcacgtccgt tataaatatg atggctatta
    19681 gaaagtcatt aaatttataa gggtgcgcaa tggccgttaa cttactgaaa aagaactcac
    19741 tcgcgctggt cgcttctctg ctgctggcgg gccatgtaca ggcaacggaa ctgctgaaca
    19801 gttcttatga cgtctcccgc gagctgtttg ccgccctgaa tccgccgttt gagcaacaat
    19861 gggcaaaaga taacggcggc gacaaactga cgataaaaca atctcatgcc gggtcatcaa
    19921 aacaggcgct ggcgatttta cagggcttaa aagccgacgt tgtcacttat aaccaggtga
    19981 ccgacgtaca aatcctgcac gataaaggca agctgatccc ggccgactgg cagtcgcgcc
    20041 tgccgaataa tagctcgccg ttctactcca ccatgggctt cctggtgcgt aagggtaacc
    20101 cgaagaatat ccacgattgg aacgacctgg tgcgctccga cgtgaagctg attttcccga
    20161 acccgaaaac gtcgggtaac gcgcgttata cctatctggc ggcatggggc gcagcggata
    20221 aagctgacgg tggtgacaaa gccaaaaccg aacagtttat gacccagttc ctgaaaaacg
    20281 ttgaagtgtt cgataccggc ggtcgtggcg caaccaccac ttttgccgag cgtggcctgg
    20341 gcgatgtgct gattagcttt gaatcggaag tgaacaacat ccgtaaacag tatgaagcac
    20401 agggctttga agtggtgatc ccgaaaacca atattctggc ggaattcccg gtggcgtggg
    20461 tcgataaaaa tgtgcaggcc aacggtacgg aaaaagccgc aaaagcctac ctgaactggc
    20521 tctacagccc acaggcgcag accatcatca ccgactatta ctaccgtgtg aataacccgg
    20581 aagtcatgga caaactgaaa aacaaattcc cgcagaccga gctgttccgc gtggaagaca
    20641 aatttggctc ctggccggaa gtgatgaaaa cccacttcac cagcggcggc gagttagaca
    20701 agctgttagc ggcggggcgt aattaatgtt tgcagtttca tcaagacgcg tgctgccggg
    20761 ctttacctta agcctcggca ccagtctgct gtttgtgtgc ctgattttgc tgctgccgct
    20821 ctccgcgctg gtgatgcagc tggctcagat gagctgggcg cagtactggg aggtgatcac
    20881 caacccgcag gtagtagcgg cctacaaagt aacgctgctg tcagcgtttg tggcatcgat
    20941 ttttaacggc gttttcggtc tgctgatggc gtggatccta acccgctatc gcttcccagg
    21001 ccgcacgctg cttgatgcgc tgatggattt accctttgcg ctgccaacgg cagtcgccgg
    21061 tttaacgcta gcctcgctct tttctgtaaa cggtttttac ggtgaatggc tggcgaagtt
    21121 tgatatcaaa gtcacctata cctggctggg gattgcggtg gcgatggcct ttaccagcat
    21181 tccgtttgtg gtacgtaccg tgcagccggt gctggaagag ttaggcccgg aatatgaaga
    21241 agcggcggaa acgcttggtg caacgcgctg gcagagtttc tgcaaagtgg tgctgccgga
    21301 gctttctccg gcgctggtgg cgggcgtggc gctgtcgttt acccgtagtc ttggtgaatt
    21361 tggcgcggtg atttttatcg ccggaaatat cgcgtggaag acggaagtga cgtcgctgat
    21421 gatttttgtg cgcttacagg agtttgatta cccggcagcg agcgcgattg cttcggtgat
    21481 cctcgcggca tctctgctgc tgctgttctc aattaacact ctgcaaagtc gctttggtcg
    21541 gcgtgtggta ggtcattaat ggcggaagtt acccaattga agcgttatga cgcgcgcccg
    21601 attaactggg gcaaatggtt tctgattggc atcgggatgc tggtatcggc gttcatcctg
    21661 ctggtgccga tgatttatat cttcgtacag gcgttcagca aagggctgat gccggtttta
    21721 cagaatctgg ccgatccgga catgctacac gccatctggc tgacggtgat gatcgcgctg
    21781 attgccgtac cggtaaacct ggtgttcggc attctgctgg cctggctggt gacgcgcttt
    21841 aacttccctg gacgccagtt actgctgacg ctactggaca ttccgtttgc cgtatcgccg
    21901 gtggttgccg gtctggtgta tctgctgttc tacggctcta acggcccgct gggcggttgg
    21961 ctggatgaac ataatctgca aattatgttc tcctggccgg gaatggtgct ggtcaccatc
    22021 ttcgtgacgt gtccgtttgt ggtgcgcgaa ctggtgccgg tgatgttaag ccagggcagt
    22081 caggaagacg aggcggcgat tttgcttggc gcgtctggct ggcagatgtt ccgtcgtgtc
    22141 acattgccga acatccgctg ggcgctgctt tatggcgtag tgctgaccaa cgcccgcgcg
    22201 attggcgagt ttggcgcggt gtcggtggtt tccggctcga ttcgcggcga aacactttcg
    22261 ctgccgttac agattgaatt gctggagcag gactacaaca ccgtcggctc ctttaccgct
    22321 gcggcactgt taacgctgat ggcgattatc accctgtttt taaaaagtat gttgcagtgg
    22381 cgcctggaga atcaggaaaa acgcgcacag caggaggaac atcatgagca ttgagattgc
    22441 caatattaag aagtcgtttg gtcgcaccca ggtgctgaac gatatctcac tggatattcc
    22501 ttcaggtcag atggtcgcgt tgctggggcc gtccggttcc gggaaaacca cgctgctgcg
    22561 cattatcgcc gggctggagc atcaaaccag cgggcatatt cgcttccacg gcaccgacgt
    22621 cagccgcctg cacgcgcgcg atcgcaaggt cggtttcgtg ttccagcatt acgcgctgtt
    22681 ccgccatatg acggtattcg acaatatcgc ttttggcctg acggtgctgc cgcgtcgcga
    22741 gcgcccgaat gccgcagcca tcaaagcgaa agtgacaaaa ttgctggaaa tggtccagct
    22801 tgcgcatctg gcggatcgtt atccggcgca gctttccggc ggccagaaac agcgtgtggc
    22861 gctggcgcgc gcgctggctg tagaaccgca aattctgctg cttgatgaac cgtttggcgc
    22921 gctggatgcg caggtgcgta aagagctgcg tcgctggctg cgtcaactgc atgaagagct
    22981 gaaattcacc agcgtgtttg tgacccacga ccaggaagag gcgaccgaag tagccgatcg
    23041 tgtagtggtg atgagtcagg ggaatattga acaggctgat gcgccgaatc aggtatggcg
    23101 cgaaccagcg acccgttttg tactcgaatt tatgggcgaa gtgaaccgcc tgcagggaac
    23161 cattcgcggc gggcagttcc atgttggcgc acatcgctgg ccgctgggct acacacctgc
    23221 ttatcagggg ccggtggatc tcttcctgcg cccgtgggaa gtggatatca gccgccgtac
    23281 cagtctcgat tcgccgctgc cggtacaggt actggaagcc agcccgaaag gtcactacac
    23341 ccaattagtg gtgcagccgc tggggtggta caacgaaccg ctgacggtcg tgatgcatgg
    23401 cgacgatgcc ccgcagcgtg gcgagcgttt attcgttggt ctgcaacatg cgcggctgta
    23461 taacggcgac gagcgtatcg aaacccgcga tgaggaactt gctctcgcac aaagcgcctg
    23521 ataggttgag tgaatgttaa acgcccggag gcgcttcccg cgatccgggc tttttaatgg
    23581 caaggtttgt aacctgtaga cctgataaga cgcgcaagcg tcgcatcagg caacaccacg
    23641 tgtggataga gatcgtgagt acattagaac aaacaatagg caatacgcct ctggtgaagt
    23701 tgcagcgaat ggggccggat aacggcagtg aagtgtggtt aaaactggaa ggcaataacc
    23761 cggcaggttc ggtgaaagat cgtgcggcac tttcgatgat cgtcgaggcg gaaaagcgcg
    23821 gggaaattaa accgggtgat gtcttaatcg aagccaccag tggtaacacc ggcattgcgc
    23881 tggcaatgat tgccgcgctt aaaggctatc gcatgaaatt gctgatgccc gacaacatga
    23941 gccaggaacg ccgtgcggcg atgcgtgctt atggtgcgga actgattctt gtcaccaaag
    24001 agcagggcat ggaaggtgcg cgcgatctgg cgctggagat ggcgaatcgt ggcgaaggaa
    24061 agctgctcga tcagttcaat aatcccgata acccttatgc gcattacacc accaccgggc
    24121 cggaaatctg gcagcaaacc ggcgggcgca tcactcattt tgtctccagt atggggacga
    24181 ccggcactat caccggcgtt tcacgcttta tgcgcgaaca atccaaaccg gtgaccattg
    24241 ttggcctgca accggaagaa ggcagcagta ttcccggcat tcgccgctgg cctgcggaat
    24301 atctgccggg gattttcaac gcttctctgg tggatgaggt gctggatatt catcagcgcg
    24361 atgcagaaaa caccatgcgc gaactggcag tgcgggaagg aatattctgt ggcgtcagct
    24421 ccggcggcgc ggttgccgga gcactgcggg tggcaaaagc taaccctggt gcggtggtgg
    24481 tagcgatcat ctgcgatcgt ggcgatcgct acctttccac cggggtgttt ggggaagagc
    24541 attttagcca gggggcgggg atttaagatt attgcgcagg caccgtttta tccgtctgcg
    24601 caattagcgc atgtaaaaaa ggctgtgcgt tctcatcgct cttaccgggg atcttcacaa
    24661 tatacggctt cccggtaatc gatgacgacg aagcaacctt atcaataaac tgctcggcag
    24721 tgtcaatgcg gttacgggta ttgcccagct tcagacgcag atgagaaacc gcttcatcgc
    24781 aggtatgttc atcaccgttg cgcacaaata tcaaatcctt tttctgtgct aatccctcca
    24841 gcatggcgtt gatgcgggct tcttcatggg cagttaactt cgcgtaagcg gggagtgtca
    24901 ttagtagtgt aatgaccaga caaatgattt tcttcattga attaccttat ccttgttttg
    24961 tgatagccga tttgactgcc agaatcaggt aaagtttcgg ttggaatacg atgaggatca
    25021 tcggcaagga gaaaaaatta aatgagtagt ttgttgttgt ttaacgataa gagcagggcg
    25081 ctgcaggcgg atatcgtcgc cgtgcagtcg caggtggttt acggcagcgt gggcaacagc
    25141 attgccgtgc ctgctatcaa acagaacggt ctgaatgttt ttgccgtgcc gacggtattg
    25201 ctgagcaata cgccgcatta tgacactttc tacggtggtg cgattccgga tgaatggttt
    25261 agcggttatt tgcgtgcgct tcaggagcgt gatgcgctgc gccagctgcg cgctgtaacc
    25321 acgggctata tgggaacggc atcgcaaatc aaaatccttg ccgagtggct gactgcgcta
    25381 cgcaaagacc atcctgacct attgatcatg gtcgatccgg tgattggcga tattgatagc
    25441 ggaatttatg tcaaacctga ccttcccgag gcgtatcgac aatatttact gccgctggcg
    25501 cagggaatta cccccaatat ctttgagctg gaaatcctga ccggtaaaaa ttgccgcgat
    25561 ctcgacagtg ccattgctgc cgcaaaaagt ctgctttcag acacattaaa atgggtggtg
    25621 attaccagcg cctccggtaa tgaagaaaat caggagatgc aggtggtggt ggtcagtgcc
    25681 gacagcgtga atgtcatttc ccattcacgg gtaaaaaccg acctgaaagg gactggcgac
    25741 ctgttttgtg ctcagcttat cagtggtttg ctgaaaggga aggcgttaaa cgatgcggtg
    25801 caccgtgcgg ggttgcgcgt actggaagtg atgcgctaca cccagcagca tgagagcgat
    25861 gaattgattt tgccgccgct ggcggaagca taaaaaatgg cgccgatggg cgccattttt
    25921 cactgcggca agaattactt cttgatgcgg ataaccgggg tttcacccac ggttacgcta
    25981 ccggacagtt tgatcagttc tttgatttcg tccatgttgg agataacaac cggagtcagg
    26041 gtagacttgg ctttctcttc cagtagcggc agatcaaatt caatgacagt atcgccaact
    26101 ttcacgcgct gaccttcttc agcaatacgc ttgaagcctt cgcctttcag ttcaacggtg
    26161 tcgataccga agtggacgaa cagttcaacg ccgctatcag attcgataga gaacgcgtga
    26221 ttggtttcaa agattttacc aatggtaccg tctacaggcg cgaccatttt gttacccgtt
    26281 ggtttgatag caataccatc accaacgatt ttttccgcaa aaacgacatc cggcacgtct
    26341 tcgatattga cgatctcgcc agagagcgga gcaatgatct caatagttcc ggtatccttc
    26401 ttgtcgtcgg aaaccagaga tttcagttta tcgaacaaac ccatgatctt ctcctaagca
    26461 gtaaattggg ccgcatctcg tggattagca gattgttttt tcttcaatga acttgttaac
    26521 cagcgtcatt aactcgtccg ttgtcggttg agcaagagcc tgctctgcta acaccttcgc
    26581 atcttcgaag ttcgtgttac ggataatctt cttaatgcgc gggatagaaa tggcgctcat
    26641 agagaattcg tccagaccca tccccagcaa cagaagtgta gcacgttcat cgccagcaag
    26701 ctcaccacac atgccagtcc atttgccttc agcatgagaa gcatcaataa cttgcttgat
    26761 caagttcagc acggacggtg acattggttg gtaaaggtgt gaaatcatat cattaccacg
    26821 gtcaactgcc agagtgtact gcgttaaatc attggtgccg atactaaaga aatcaacttc
    26881 tttggctaaa tgacgtgcaa ttgttgcggc agccggtgtt tccaccatta cgccgatttc
    26941 aattgactcg tcaaacgctt taccttcgtc gcgcagttcc tgtttgtaga tttcgatctc
    27001 tttgcgcagt gcacgcactt cttcaacaga gatgatcatc gggaacataa tgcgcaattt
    27061 accgaaagcc gaggcacgca ggatagcgcg gagctgatcg cgcaggatct ctttacgatc
    27121 catcgcgata cggatagcgc gccagccgag gaacgggttc tcttctttcg ggaagttcat
    27181 gtatggcagc tctttgtcgc cgccgatgtc catggtacga acgataaccg cctgcgagcc
    27241 acacgcttca gccactgctt tgtaagcagc aaactgttct tcttcagtgg gcagcgcgtc
    27301 gcggtccatg aacaggaact cagtacgata cagaccaacg ccttcagcgc cgttacgctc
    27361 tgcaccttca acgtcacgaa ccgtaccaat gttagcgcac acttctacct ggtgaccgtc
    27421 cagcgtaata gctggcagat ctttcagttt agcaagctct gctttttcag aagccacttg
    27481 ctcctgaaca gcgcgcattt tatcaataac ttcgttggtt ggattgacgt aaacctgatt
    27541 atttacggca tccagaatca gatagtcgtc atttttcacc tgagaggtga cgctaccggt
    27601 acccacgata gcaggcagtt ccagagaacg cgccatgata gaggtgtggg aagtacggcc
    27661 acccgcgtcg gtgatgaaac ccagcacctt cttcaggttc agctgtgcgg tttcggacgg
    27721 cgtcaggtca gcggcaacca gaatgacttc atcctgaatg gcgctcaggt cgataatctt
    27781 caggcccagg atgttgcgca gcaggcgctt accgatatca cgtacgtcag ccgcacgttc
    27841 tttcaggtat tcatcatcca gctcttccag ggcagaagcc tgaccttcga taacttcatg
    27901 agcagctgcg tcagctgtca tgtgcttatc tttaatcagg gctatgattt cctgctccag
    27961 ctcctcatct tcgagcagca taatatgccc ttcaaagatg gcttcttttt cttcaccgaa
    28021 cgtttcacca gctttcgttt tgatcgtttc cagctgggct gatgccttgg cacgaccgct
    28081 cagaaaacgt tcaacttcct gatcaacctg gtcggcagaa atttttttcc ggtcaatgac
    28141 aatttcgtct tctttcagaa gcagagcttt accgaaagcg atacccgggg atgctaaaat
    28201 gcctgaaatc ataaccctac cttacttgtg actgattttt aaaagaaccc gggaaattac
    28261 tcgagttccg ccatcagttt aaccagatgt tcaaccgctt tctgctcgtc ttcgccttct
    28321 gcggagatag tcacaacggt accttgagtc aggcccagag tctgcagttt aaacaggctt
    28381 ttcgcgctgg cgcttttgcc gttggaagtc acagtaattt cagaagtgaa gcccttagct
    28441 tcttttacaa actgggcagc agggcgggtg tgcagaccgt tcggagcggt aatggtaact
    28501 tcttgctgga acattgtatt tccccaactt ataggtttag tgttgtggaa ctaaagtcta
    28561 gcctggcggt tcgactttag cctgtattgt tagcgccagt ttttaacaga cgcgacgcac
    28621 gaagagcgca atacagtcca ttgctggcgg gtgttgaccg ctgacgtttc gtacgtcatt
    28681 aaacattatg ccgctaaagg aagaattgaa ccaaatcata aaatcgattc agctttggct
    28741 tttcctgtaa cgattaattt cgcgcatcaa aataattagt ctggttaaat accagttccg
    28801 acaggctgaa tcaatgccag gaggggcgaa agtttgaagc aggccacaaa aaagcaccta
    28861 aaaaggtgct tttttacgca tttttaacaa gctggcatta ctgttgcagt tctttctcag
    28921 tgaagagatc ggcaaacaat gcggtgctta aataacgctc acccgatgac ggtagaataa
    28981 ccacaatatt cttgttggta aagctttcat cttcttgtag tttcaacgcc gcagcaacag
    29041 ctgctccaga agagatacct gcaagaatac cttcttcttc catcagacga cgcgcggtag
    29101 aaatcgcttc ttcattggtg atgccaatga ctttatcgac cagcttgaga tcgaggttag
    29161 ctgggataaa accagcgcca ataccctgaa ttttatgcgg gccaggttta atctcttcac
    29221 cagccagtgc ctgggcgata actggagaat cggttggctc aacggcgaca gagataagat
    29281 cggtcttgcc tttggtgcct ttaatgtagc ggctgacgcc agtaagcgta ccgccagtcc
    29341 caacgccagc aataaataca tcaacctgac cgtcggtatc ttcccagatc tccggaccgg
    29401 tggttttttc gtgaatttca gggtttgccg gattgctgaa ttgttgcagc agcaggtatt
    29461 tctctggatt gctggcgaca atttcttctg ctttttggat tgcgcctttc atgcctttag
    29521 caccttccgt cagcaccagg tttgcaccta acgctttcag cagcttgcgg cgttcaatac
    29581 tcatggtttc tggcatggtc agggtgagtt tgtaaccgcg agcggcagct acataggcca
    29641 gtgcaatccc ggtattaccg ctggttggtt caaccagttc aacgcctggt ttcagcacgc
    29701 cgcgcttttc ggcatcccaa atcatgttgg caccgatacg gcacttaacg ctgaagctgg
    29761 ggttacgaga ttccaccttc gccagaatgc gtccgttacc gatgcgattc aggcgaacca
    29821 gcggcgtgtg accgatagtc agcgagttat cttcaaaaat cttactcatg gcctgtcctt
    29881 aactgtatga aattgggata caacaggtag catacccgct cagagaatat gcggaagtaa
    29941 ggatttagca tatctatata cagaagggaa ataatgacat gcaagatgga ataaggggcg
    30001 gcataagcca cccctgtttc acacaaacgg tttacaaaat aaccggtaga ttgttaccgc
    30061 cacatcgcgt gtttatcgcg atagcaatcg acccacatcg ccgtcgcgcc acaaacggca
    30121 acgggcatga tgaacagatt aagcagcggg atcatcgtaa acaggctggt taaagcacca
    30181 aactgcatat tggtgatttt gcgtgtgcgc agggcggtgc gcatctcttt aaacggcact
    30241 ttgtggttat cgaaggggta atcgcaatac tggatggcta acatccaggc gctaaacagg
    30301 aaccacagta ccggcgcgac ggtttgccca ataccaggga ttaagtaaag aattagcaat
    30361 acaattgcgc gcggcagata ccaggcaaat ttttgccatt cgcgtttcat gattcgcggc
    30421 acatctttca tgataccgaa aatcccggta tctggcggtg tagcgccagt caatcgtgct
    30481 tccagttgtt cagccaataa accgttaaac ggagcggcaa tccagttagc aatcgtggag
    30541 aagaaatagc caaacactaa cagcacagag atgacagcca gaggccacaa cagataactc
    30601 agccattgta gccagtccgg aacgtaactc atgagagtcg ggatccagac atcgagctgt
    30661 gtaaagagcc accagaatgc gccccccatc aacaaaatat tgaccagcag cggtaaaata
    30721 acgaaacgcc gaatcccagg ttgcgagacg agcttccagc cttgcgcaaa atagtaaaaa
    30781 ccgctgcgtg gggcagatgt gaatgatgaa accataatca ggatgagctc cttttgacca
    30841 atcccaggaa aattctgcgt attttaccgg gtaattgcgc aatggacagt taggatatgt
    30901 tcgaaaaaac agcaaaaagc acgatttcat ctatctttgt gctgtgaaag ttaatagtgc
    30961 acttgcactt gaggtaatcg gcaaatactc ttagtgagta aatgtttgcc gtggtggcaa
    31021 ggtgttagaa caacagagaa tataatgatg caggatttgc gtctgatatt aatcattgtt
    31081 ggcgcgatcg ccataatcgc tttactggta catggtttct ggaccagccg taaagaacga
    31141 tcttctatgt tccgcgatcg gccattaaaa cgaatgaagt caaaacgtga cgacgattct
    31201 tatgacgagg atgtcgaaga tgatgagggc gttggtgagg ttcgtgttca ccgcgtgaat
    31261 catgccccgg ctaacgctca ggagcatgag gctgctcgtc cgtcgccgca acaccagtac
    31321 caaccgcctt atgcgtctgc gcagccgcgt caaccggtcc agcagccgcc tgaagcgcag
    31381 gtaccgccgc aacatgctcc gcgtccagcg cagccggtgc agcagcctgc ctatcagccg
    31441 cagcctgaac agccgttgca gcagccagtt tcgccacagg tcgcgccagc gccgcagcct
    31501 gtgcattcag caccgcaacc ggcacaacag gctttccagc ctgcagaacc cgtagcggca
    31561 ccacagcctg agcctgtagc ggaaccggct ccagttacgg ataaaccgaa gcgcaaagaa
    31621 gcggtgatta tcatgaacgt cgcggcgcat cacggtagcg agctaaacgg tgaactgctt
    31681 cttaacagca ttcaacaagc gggcttcatt tttggcgata tgaatattta ccatcgtcat
    31741 cttagcccgg atggcagcgg cccggcgtta ttcagcctgg cgaatatggt gaaaccggga
    31801 acctttgatc ctgaaatgaa ggatttcact actccgggtg tcaccatctt tatgcaggta
    31861 ccgtcttacg gtgacgagct gcagaacttc aagctgatgc tgcaatctgc gcagcatatt
    31921 gccgatgaag tgggcggtgt cgtgcttgac gatcagcgcc gtatgatgac tccgcagaaa
    31981 ttgcgcgagt accaggacat catccgcgaa gtcaaagacg ccaacgcctg atacacttaa
    32041 ggcaaattaa ctcctcttcg aacccccgct tgtcgggggt ttttagcatt gatggtgcga
    32101 tatggaatca atcgaacaac aactgacaga actgcgaacg acgcttcgcc atcatgaata
    32161 tctttatcat gtgatggatg cgccggaaat tcccgacgct gaatacgaca ggctgatgcg
    32221 cgaactgcgc gagctggaaa ccaaacatcc agaactgatt acgcctgatt cgcctaccca
    32281 acgtgtaggc gctgcgccgc tggcggcttt cagccagata cgccatgaag taccaatgct
    32341 gtcactggat aacgtttttg atgaagaaag ctttcttgct ttcaacaaac gtgtgcagga
    32401 ccgtctgaaa aacaacgaga aagtcacctg gtgctgtgag ctgaagctgg atggccttgc
    32461 cgtcagtatt ctgtatgaaa atggcgtttt agtcagtgcc gcgacccgtg gcgatggcac
    32521 caccggggaa gatatcacgt ctaatgtgcg tactattcgc gctattccgc tgaagctgca
    32581 cggagagaat atcccggcgc gtctggaagt gcgtggtgaa gtgttcctgc cgcaggcggg
    32641 gttcgaaaag attaacgaag atgcgcgacg cacgggcggg aaagtttttg ctaacccacg
    32701 taatgcggca gctggttcac tgcgtcagct tgatccgcgt attacagcga agcgaccgct
    32761 cacttttttc tgctatggcg ttggtgttct ggaaggtggc gagctgccgg atactcatct
    32821 tggccgttta ctgcaattta aaaagtgggg gttgccggtc agcgatcggg taacgctttg
    32881 tgaatcggcg gaagaagtgc tggcgttcta tcacaaagtg gaagaagacc gcccgacgct
    32941 gggctttgat atcgacggcg tggtgattaa ggtcaactca ctggaacagc aggagcagct
    33001 tggctttgtc gcgcgtgccc cgcgctgggc ggtagcgttt aaattcccgg cgcaggagca
    33061 gatgaccttt gtgcgtgacg tcgagtttca ggttgggcgt actggcgcga ttacgcctgt
    33121 tgcgcgtctg gaacctgtcc atgttgctgg cgtgctggtg agtaacgcaa ccttacacaa
    33181 tgcggatgaa atcgaacgtc ttggtttacg cattggcgat aaagtggtga ttcgccgcgc
    33241 tggcgacgtg atcccgcagg tggttaacgt cgtgctttct gaacgcccgg aagatacccg
    33301 tgaggttgta ttcccaacgc attgtccggt atgtggttct gacgttgagc gtgtggaagg
    33361 tgaagcggtt gcccgctgta ccggtggcct gatttgcggt gcgcagcgta aagagtcgct
    33421 gaaacacttt gtttcccgcc gtgcgatgga tgttgacgga atgggcgaca aaatcatcga
    33481 tcagctggtt gaaaaagaat atgtccatac tccggcagat ctgttcaaac tcaccgcagg
    33541 caaactgacc ggactggagc gtatggggcc aaaatcggca caaaacgtgg ttaacgcgct
    33601 agaaaaagcg aaagaaacca cctttgctcg cttcctctat gcacttggca tccgtgaagt
    33661 cggcgaggcc accgcagcag gtctggcggc atatttcggc acgctggaag cgctggaagc
    33721 cgcttcgatt gaagagctgc aaaaggtgcc tgatgttggc attgtcgttg catcccacgt
    33781 tcacaacttc tttgccgaag aaagcaaccg caatgttatc agcgagctgt tggcggaagg
    33841 tgtccactgg cctgcgccga tcgttatcaa cgcggaagag attgacagcc cgtttgctgg
    33901 taaaaccgtg gtgcttacgg gcagcttaag tcagatgtca cgtgatgacg ctaaagctcg
    33961 actggtcgaa ctgggcgcga aagtcgcggg cagcgtgtcg aagaaaaccg atctggtgat
    34021 agcgggtgaa gctgcaggat ctaaactggc gaaggcgcag gaactgggca ttgaagtcat
    34081 cgacgaagcg gaaatgctgc gtttgctggg tagctgagat ggaaaaagag cagctgattg
    34141 aaatagccaa tacgataatg ccgtttggca aatacaaagg gcgtcgctta atcgacctgc
    34201 cggaggagta tctgctgtgg tttgcccgca aagatgagtt cccggcaggg aagctcggtg
    34261 agctaatgca aatcacgctg ctgattaaaa ccgaggggct gacgcaactg gtccagcccc
    34321 tgaagcgtcc gctttaagct ttatcggtgc tgctttcctg ctgcgcctgt aactgttcgg
    34381 tctggcgttt gtatcgacgc gccagcaccg cacagaccat caattggatc tgatggaaaa
    34441 tcatcagggg cagcaccatc ataccgatca ccgatgtggg gaacagaatg tttgccatcg
    34501 ggatgccatt tgccagactc tttttcgaac cacaaaagac gatagtaatt tcatctgcct
    34561 tattgaagcc cagtcggcgt gccataaaga cgttaactac aatcacgata gccagaagaa
    34621 cgcagctgac caccacgata aacagcaatg atccccagcc aactttatgc cagataccat
    34681 taacgacggc ttcgctgaac gctgtataaa ccaccaacag aatggacgtc tggtcagttt
    34741 tcgcaatcca ttttttattg cgcgacaccc agtcaccaat ccacggccgg gaaagatgcc
    34801 ccaacacaaa cggcagcagc agttgcagca taattttacc gacctgctca aggctgcccc
    34861 ctgcaccgtg aacattcatc accagaccaa ccagcaatgg tgaaaggaaa atccccagca
    34921 ggctggatgc tgacgcagaa caaaccgccg ccgcgacgtt accgcccgcc attgacgtga
    34981 aggcgattgc agactgcacg gtagccggga gaatgcacaa gtagagaaaa ccggagtaga
    35041 gcatcgggtc gacatttacc ggtttccacc aggcaaacag tacacccaga atcggaaaca
    35101 gcacgaaggt gctgcacatt acccacaaat gcagtcgcca gtgaccaccg ccagcaataa
    35161 tcgcctcacg cgacaacttc gcgccgtgca taaagaacag cagggcaatc gctgcggtgg
    35221 tcagattttc aaagaagggg acgaaatcgc ctctggccgg aaagaaagag gccagcaaca
    35281 ccaccgtgat cagggttaag gtgaaaggat cgaggatacg aaaaagtttc ataaagactc
    35341 cagatgatcg atggttctat tgtgcttctt ccagtttgag aaataaaatt gatttattgc
    35401 atccattcat gaagaaaaca gatgaattat tctttaaaac aattaaaggt tttcgtcaca
    35461 gtagcgcagg agaaaagttt tagtcgtgca ggagagcgta tcggcctgag ccagtcggca
    35521 gtgagtcaca gtgtgaagga actggaaaat catactggtg ttcgcctgct ggacagaacc
    35581 acgcgtgaag tggtgcttac agatgcaggg cagcagctgg ctttgcgtct tgagcgactg
    35641 ctggatgaac tgaacagcac gttgcgcgat accgggcgta tggggcaaca actgagcgga
    35701 aaagttcggg tcgctgccag ccagaccatt tccgcgcatc ttattccgca atgcattgct
    35761 gaaagccatc gccgctatcc agatattcag tttgtcctgc acgatcgtcc gcagcagtgg
    35821 gtgatggaaa gtattcgcca gggagatgtc gactttggca tcgtcatcga tccaggccca
    35881 gtgggcgatc tgcaatgtga agcgattctt tccgagcctt tctttctgct ttgccatcgc
    35941 gatagcgctt tggccgtcga agattatgta ccctggcagg cattacaggg agcgaaattg
    36001 gtgttgcagg attacgcgtc aggcagccga ccgctgattg acgcagcgct ggcgcgcaat
    36061 ggtattcagg cgaatattgt gcaggagatt ggacatccgg cgacgctgtt cccgatggta
    36121 gccgcaggca tcggcatcag tattcttccc gcactggcgc taccattgcc tgaaggtagc
    36181 ccactggtgg taaagcgcat tacgccggtt gttgaacgcc agttgatgct ggtgcgcagg
    36241 aagaaccgtt cactttccac cgccgctgaa gcgttatggg acgtggtacg agaccagggc
    36301 aatgcgctaa tggctggtcg ggaaggggat ccgttgtatc agatatagag tgaattcata
    36361 ctgccgggag ccccggcagt aatcaatcag aagttaaatt tgatggagta gattatcgca
    36421 tcctggtaaa aatcttcgcc cagtttgtca tccgcatatc gatactgaat ccccgtagta
    36481 atgtgtgatg ttgcattcca ccacagtgca actgcgccat tcagcccttc tttaccgcca
    36541 ttacccgctg cataggtagc gtcacggtca aactcgtact cgttccagtt ggtaagagtg
    36601 aatttctcgc tgcccagcat aaagttgtaa cctgcaaccc agccggcgac atagccgttg
    36661 tcgccagtgt aataggtttg atctgtataa cgcttagcaa agaacggttt gaaccaccaa
    36721 ccgctgccgg tgaaattgta gccgataccg tacaggaaca tatcgtcgtg gaaattcacg
    36781 cgattcgcag aaccgtaggt gccatacgca tgcaagtaga gattaaatcc ggtgtcaccc
    36841 aggtaaatac ggttggtatt tttaaaggta taacgctgct cgctacctgg tttattatgg
    36901 cgaccgttat agaagttttc ccagtcgaag aacccgtaca tttctcccca actaaagtta
    36961 gcgccacctt caaattcaag ataaccaaaa tcatctttgt gtgatttggt cgacgatttt
    37021 tcggtagtcc ggctggtcca gtccagataa tgcacgccga tatctgcaaa accgccttta
    37081 aattgtgcat gagatacaac aggtatcgct aatatagagg aaagtgtcag agttaaaaga
    37141 tgttttttca tagcataatt ccctatgccg atcgttatta gtagattgct taataaagtg
    37201 gtttgctaaa aacgacatat tgttaatgca aaggaattaa tatcgccaat acagttttta
    37261 ccgtggatta atagagaaaa gatataaatc catcgatatc gaatcgctat tgaatgcgtg
    37321 agaaaagtgt attggtaaga gccgggacaa cctcgctata aaaaggcgtt agattccacc
    37381 ctacagaaaa aggatatgta tgtctcaggt tcaattttct cataacccgc tgttttgcat
    37441 agatattatc aagacttata aacctgattt cacgccacga gtggccttta ttttaggttc
    37501 cgggctcggc gcgctggccg atcagattga gaacgctgtc gcaatttcct acgaaaagct
    37561 gccgggcttc ccggtaagta cggtacatgg tcatgcgggg gagttagtgc tgggccatct
    37621 ccagggcgta ccggtggtat gtatgaaagg tcgcggacat ttctacgaag gtcgtggaat
    37681 gaccattatg actgacgcaa ttcgtacctt taagctgctg ggctgcgagc tactgttctg
    37741 caccaatgcg gcaggctcgc tgcgtccgga agtgggggca ggcagcctgg tcgcattgaa
    37801 agatcatatc aacaccatgc ccggtacgcc gatggtgggt cttaacgatg atcgttttgg
    37861 agagcgcttc ttctcactgg caaatgccta cgatgcggaa taccgcgcac tgttacaaaa
    37921 agtggcgaaa gaagaggggt tccctctgac ggagggcgtg ttcgtctcgt atccggggcc
    37981 gaatttcgag actgcggcgg aaattcgcat gatgcaaatt attggtgggg atgttgttgg
    38041 tatgtctgtg gtgcctgagg ttatttcagc tcgccattgc gaccttaaag tcgttgcggt
    38101 ctctgcgatt accaatatgg cggaaggttt aagcgatgtg aaattgtcac atgcgcaaac
    38161 gctggcggct gcggagctct ctaagcagaa cttcatcaac cttatttgtg gctttctgcg
    38221 caaaattgcc tgagaaaaac aataagaaac ggcctcgcgg gtgaggccaa atgatgcgac
    38281 aaggaaacga ttatgagcat cgcgatgcgc ttaaaggtaa tgtccttttt gcaatatttt
    38341 atctggggga gctggctggt taccctcggc tcttacatga ttaataccct tcatttcacc
    38401 ggcgctaatg ttggcatggt ttacagttcc aaagggatcg ccgcgattat tatgcctggt
    38461 ataatgggga tcatcgcaga caaatggctg cgcgcagaac gtgcatacat gctgtgtcac
    38521 ctggtgtgtg cgggcgtact tttttatgcg gcatccgtaa ctgatccgga tatgatgttt
    38581 tgggtgatgt tagtcaatgc gatggcgttt atgccgacta ttgcgttatc gaacagcgtc
    38641 tcttattcct gtcttgccca ggcagggctt gacccggtga ccgctttccc gcccattcgc
    38701 gtttttggta cggtggggtt cattgtcgcg atgtgggcag taagcctgct gcatctggaa
    38761 ttgagtagtc tgcagctgta tatcgcgtcc ggtgcgtcat tgctgctgtc ggcttatgcg
    38821 ctgactttgc cgaagattcc ggttgcggag aaaaaagcga ccacatcgct tgccagcaag
    38881 ctgggtctgg atgccttcgt gctgtttaaa aatccacgca tggccatctt tttcctcttt
    38941 gccatgatgc tgggtgcggt actgcaaatt accaacgttt ttggtaatcc gttcctacat
    39001 gatttcgccc gtaacccgga gtttgctgac agttttgtgg tgaaatatcc ctccatttta
    39061 ctgtcagttt cacagatggc agaagtgggc tttatactga ctatcccatt ctttttaaag
    39121 cgatttggca ttaaagccgt catactgatg agtatggtgg cctggacgct gcgctttggc
    39181 ttcttcgcct atggcgatcc gtcaacaacc ggatttattt tgctgctgct gtcgatgatt
    39241 gtttatggct gtgcattcga tttcttcaat atttctggtt cggtatttgt cgaacaggaa
    39301 gttgattcca gcattcgtgc cagcgcgcag gggctcttta tgaccatggt aaatggtgtc
    39361 ggcgcatggg ttggctcgat tctgagtggc atggcagtag attacttttc ggtggatggc
    39421 gtaaaagact ggcaaactat ctggctggtg tttgcaggat atgctctttt tctcgcagtg
    39481 atatttttct ttgggtttaa atataatcat gaccctgaaa agataaagca tcgagcggtg
    39541 gctcattaaa aggattcgca gctctgctct tcagagctgc ttttatgata aaggttaatt
    39601 agtgaaagat atttt
//
LOCUS       DAZGAN020000036        38560 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGAN020000036 DAZGAN020000000
VERSION     DAZGAN020000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 38560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.036"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            134..703
                     /gene="yadM"
                     /locus_tag="ACHKYO_RS10330"
                     /old_locus_tag="pgaptmp_002066"
     CDS             134..703
                     /gene="yadM"
                     /locus_tag="ACHKYO_RS10330"
                     /old_locus_tag="pgaptmp_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4428920.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            718..1320
                     /gene="yadL"
                     /locus_tag="ACHKYO_RS10335"
                     /old_locus_tag="pgaptmp_002067"
     CDS             718..1320
                     /gene="yadL"
                     /locus_tag="ACHKYO_RS10335"
                     /old_locus_tag="pgaptmp_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HGU4428921.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            1347..1943
                     /locus_tag="ACHKYO_RS10340"
                     /old_locus_tag="pgaptmp_002068"
     CDS             1347..1943
                     /locus_tag="ACHKYO_RS10340"
                     /old_locus_tag="pgaptmp_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGU4428922.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            1927..3270
                     /locus_tag="ACHKYO_RS10345"
                     /old_locus_tag="pgaptmp_002069"
     CDS             1927..3270
                     /locus_tag="ACHKYO_RS10345"
                     /old_locus_tag="pgaptmp_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HGU4428923.1"
                     /translation="MCTTNNRSKQITATVTAVDGGVIMKKFFRHFLFLILCLSCYTAS
                     AGTDDNVGYIVGNSYGVGPSDQKWRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTG
                     VKLQWSQLDTASGGGFLYCNRSDSTSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYY
                     TLLISNMWSAYGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAV
                     GGIYQTLTIEFYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFD
                     LLNVKLTNPTCFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNI
                     ETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKD
                     YDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            3383..4177
                     /gene="panB"
                     /locus_tag="ACHKYO_RS10350"
                     /old_locus_tag="pgaptmp_002070"
     CDS             3383..4177
                     /gene="panB"
                     /locus_tag="ACHKYO_RS10350"
                     /old_locus_tag="pgaptmp_002070"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HGU4428924.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            4189..5040
                     /gene="panC"
                     /locus_tag="ACHKYO_RS10355"
                     /old_locus_tag="pgaptmp_002071"
     CDS             4189..5040
                     /gene="panC"
                     /locus_tag="ACHKYO_RS10355"
                     /old_locus_tag="pgaptmp_002071"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HGU4428925.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(5114..6015)
                     /gene="rpnC"
                     /locus_tag="ACHKYO_RS10360"
                     /old_locus_tag="pgaptmp_002072"
                     /pseudo
     CDS             complement(5114..6015)
                     /gene="rpnC"
                     /locus_tag="ACHKYO_RS10360"
                     /old_locus_tag="pgaptmp_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
     gene            6289..6669
                     /gene="panD"
                     /locus_tag="ACHKYO_RS10365"
                     /old_locus_tag="pgaptmp_002073"
     CDS             6289..6669
                     /gene="panD"
                     /locus_tag="ACHKYO_RS10365"
                     /old_locus_tag="pgaptmp_002073"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HGU4428926.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(6673..7902)
                     /gene="yadE"
                     /locus_tag="ACHKYO_RS10370"
                     /old_locus_tag="pgaptmp_002074"
     CDS             complement(6673..7902)
                     /gene="yadE"
                     /locus_tag="ACHKYO_RS10370"
                     /old_locus_tag="pgaptmp_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU4428927.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(7966..8406)
                     /gene="yadI"
                     /locus_tag="ACHKYO_RS10375"
                     /old_locus_tag="pgaptmp_002075"
     CDS             complement(7966..8406)
                     /gene="yadI"
                     /locus_tag="ACHKYO_RS10375"
                     /old_locus_tag="pgaptmp_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU4428928.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(8511..9281)
                     /gene="yadH"
                     /locus_tag="ACHKYO_RS10380"
                     /old_locus_tag="pgaptmp_002076"
     CDS             complement(8511..9281)
                     /gene="yadH"
                     /locus_tag="ACHKYO_RS10380"
                     /old_locus_tag="pgaptmp_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4428929.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(9278..10204)
                     /gene="yadG"
                     /locus_tag="ACHKYO_RS10385"
                     /old_locus_tag="pgaptmp_002077"
     CDS             complement(9278..10204)
                     /gene="yadG"
                     /locus_tag="ACHKYO_RS10385"
                     /old_locus_tag="pgaptmp_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4428930.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            10313..10975
                     /gene="can"
                     /locus_tag="ACHKYO_RS10390"
                     /old_locus_tag="pgaptmp_002078"
     CDS             10313..10975
                     /gene="can"
                     /locus_tag="ACHKYO_RS10390"
                     /old_locus_tag="pgaptmp_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HGU4428931.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(11016..11552)
                     /gene="hpt"
                     /locus_tag="ACHKYO_RS10395"
                     /old_locus_tag="pgaptmp_002079"
     CDS             complement(11016..11552)
                     /gene="hpt"
                     /locus_tag="ACHKYO_RS10395"
                     /old_locus_tag="pgaptmp_002079"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGU4428932.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            11758..14148
                     /gene="gcd"
                     /locus_tag="ACHKYO_RS10400"
                     /old_locus_tag="pgaptmp_002080"
     CDS             11758..14148
                     /gene="gcd"
                     /locus_tag="ACHKYO_RS10400"
                     /old_locus_tag="pgaptmp_002080"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HGU4428933.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQL
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(14195..15745)
                     /gene="cueO"
                     /locus_tag="ACHKYO_RS10405"
                     /old_locus_tag="pgaptmp_002081"
     CDS             complement(14195..15745)
                     /gene="cueO"
                     /locus_tag="ACHKYO_RS10405"
                     /old_locus_tag="pgaptmp_002081"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HGU4428934.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            15911..16258
                     /gene="yacC"
                     /locus_tag="ACHKYO_RS10410"
                     /old_locus_tag="pgaptmp_002082"
     CDS             15911..16258
                     /gene="yacC"
                     /locus_tag="ACHKYO_RS10410"
                     /old_locus_tag="pgaptmp_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HGU4428935.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            16364..17230
                     /gene="speE"
                     /locus_tag="ACHKYO_RS10415"
                     /old_locus_tag="pgaptmp_002083"
     CDS             16364..17230
                     /gene="speE"
                     /locus_tag="ACHKYO_RS10415"
                     /old_locus_tag="pgaptmp_002083"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGU4428936.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            17246..18040
                     /gene="speD"
                     /locus_tag="ACHKYO_RS10420"
                     /old_locus_tag="pgaptmp_002084"
     CDS             17246..18040
                     /gene="speD"
                     /locus_tag="ACHKYO_RS10420"
                     /old_locus_tag="pgaptmp_002084"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HGU4428937.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(18078..18440)
                     /gene="yacL"
                     /locus_tag="ACHKYO_RS10425"
                     /old_locus_tag="pgaptmp_002085"
     CDS             complement(18078..18440)
                     /gene="yacL"
                     /locus_tag="ACHKYO_RS10425"
                     /old_locus_tag="pgaptmp_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HGU4428938.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(18616..21213)
                     /gene="acnB"
                     /locus_tag="ACHKYO_RS10430"
                     /old_locus_tag="pgaptmp_002086"
     CDS             complement(18616..21213)
                     /gene="acnB"
                     /locus_tag="ACHKYO_RS10430"
                     /old_locus_tag="pgaptmp_002086"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HGU4428939.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            21568..23325
                     /gene="yacH"
                     /locus_tag="ACHKYO_RS10435"
                     /old_locus_tag="pgaptmp_002087"
     CDS             21568..23325
                     /gene="yacH"
                     /locus_tag="ACHKYO_RS10435"
                     /old_locus_tag="pgaptmp_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HGU4428940.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPSN
                     TVSTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(23396..24820)
                     /gene="lpdA"
                     /locus_tag="ACHKYO_RS10440"
                     /old_locus_tag="pgaptmp_002088"
     CDS             complement(23396..24820)
                     /gene="lpdA"
                     /locus_tag="ACHKYO_RS10440"
                     /old_locus_tag="pgaptmp_002088"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGU4428941.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(25145..27037)
                     /gene="aceF"
                     /locus_tag="ACHKYO_RS10445"
                     /old_locus_tag="pgaptmp_002089"
     CDS             complement(25145..27037)
                     /gene="aceF"
                     /locus_tag="ACHKYO_RS10445"
                     /old_locus_tag="pgaptmp_002089"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HGU4428942.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(27052..29715)
                     /gene="aceE"
                     /locus_tag="ACHKYO_RS10450"
                     /old_locus_tag="pgaptmp_002090"
     CDS             complement(27052..29715)
                     /gene="aceE"
                     /locus_tag="ACHKYO_RS10450"
                     /old_locus_tag="pgaptmp_002090"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HGU4428943.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(29876..30640)
                     /gene="pdhR"
                     /locus_tag="ACHKYO_RS10455"
                     /old_locus_tag="pgaptmp_002091"
     CDS             complement(29876..30640)
                     /gene="pdhR"
                     /locus_tag="ACHKYO_RS10455"
                     /old_locus_tag="pgaptmp_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HGU4428944.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            31181..32554
                     /gene="aroP"
                     /locus_tag="ACHKYO_RS10460"
                     /old_locus_tag="pgaptmp_002092"
     CDS             31181..32554
                     /gene="aroP"
                     /locus_tag="ACHKYO_RS10460"
                     /old_locus_tag="pgaptmp_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HGU4428945.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTHFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(32597..33451)
                     /gene="ampE"
                     /locus_tag="ACHKYO_RS10465"
                     /old_locus_tag="pgaptmp_002093"
     CDS             complement(32597..33451)
                     /gene="ampE"
                     /locus_tag="ACHKYO_RS10465"
                     /old_locus_tag="pgaptmp_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HGU4428946.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(33448..33999)
                     /gene="ampD"
                     /locus_tag="ACHKYO_RS10470"
                     /old_locus_tag="pgaptmp_002094"
     CDS             complement(33448..33999)
                     /gene="ampD"
                     /locus_tag="ACHKYO_RS10470"
                     /old_locus_tag="pgaptmp_002094"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HGU4428947.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDVLFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            34087..34980
                     /gene="nadC"
                     /locus_tag="ACHKYO_RS10475"
                     /old_locus_tag="pgaptmp_002095"
     CDS             34087..34980
                     /gene="nadC"
                     /locus_tag="ACHKYO_RS10475"
                     /old_locus_tag="pgaptmp_002095"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGU4428948.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            35183..35623
                     /gene="ppdD"
                     /locus_tag="ACHKYO_RS10480"
                     /old_locus_tag="pgaptmp_002096"
     CDS             35183..35623
                     /gene="ppdD"
                     /locus_tag="ACHKYO_RS10480"
                     /old_locus_tag="pgaptmp_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HGU4428949.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            35633..37018
                     /gene="gspE"
                     /locus_tag="ACHKYO_RS10485"
                     /old_locus_tag="pgaptmp_002097"
     CDS             35633..37018
                     /gene="gspE"
                     /locus_tag="ACHKYO_RS10485"
                     /old_locus_tag="pgaptmp_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HGU4428950.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENDCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            37008..37928
                     /gene="hofC"
                     /locus_tag="ACHKYO_RS10490"
                     /old_locus_tag="pgaptmp_002098"
                     /pseudo
     CDS             37008..37928
                     /gene="hofC"
                     /locus_tag="ACHKYO_RS10490"
                     /old_locus_tag="pgaptmp_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(38057..>38560)
                     /locus_tag="ACHKYO_RS10495"
                     /old_locus_tag="pgaptmp_002099"
     CDS             complement(38057..>38560)
                     /locus_tag="ACHKYO_RS10495"
                     /old_locus_tag="pgaptmp_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HGU4428951.1"
                     /translation="YALNPNSGARVVTGTQNREYMELNRLWLHDCMPRNSESRAISYA
                     LKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVGSHESTFYELDGEWYHEICRN
                     AIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRARKRLNTKLFKVQPYPKPQTVK
                     TGLKEIG"
BASE COUNT         9511 a        10227 c         9440 g         9382 t
ORIGIN      
        1 aaatggctcg aagaaagtaa acccgtaagt tgtcttgcgc attatcaaca aagttcagaa
       61 gcagaaaaaa tagcgcaatc tattattctg aatggaattc ggtgtcagat tcagtaacta
      121 caagggacgt caaatgataa aaataacgcc acataaaata gcgatactga tgggaatatt
      181 attatcacca tcagtatttg caacggatat taatgtagag tttacagcca ctgtcaaagc
      241 gacaacctgt aacatcacac ttactggtaa taacgtcacg aatgatggca ataataacta
      301 cacattgaga atccctaaga tgggtctgga taagatcgcg aataaaacga cagaatctca
      361 ggctgatttt aaactggttg ctagtgggtg cagcagtggc atcagttgga ttgataccac
      421 tctgaccgga aatgcatcat caagctcacc taagcttatt ataccgcagt ctggtgattc
      481 atcttcgacg acaagtaata tcggtatggg tttcaaaaaa cggactactg atgatgccac
      541 tttccttaaa cctaacagtg cggaaaagat acgctggagc acagacgaga tgcagcccga
      601 taagggtctt gaaatgaccg ttgcgctacg tgaaacagat gcagggcaag gcgtaccggg
      661 gaattttcgt gcactggcaa cgtttaattt catctatcaa taagaaggat aattaagatg
      721 acatttaaaa atttacgtta tggattatcc agcagtgtcg ttttggctgc ctcattgttc
      781 agcgtaccct cttatgcggc aacagacagc attgggctga ccgtgattac taccgtagaa
      841 ataggtactt gtactgctac attagtaaat gactctgatc aggacatttc tgttgttgac
      901 tttggcgatg tatatatttc tgaaatcaat gccaagacca aagttaaaac attcaaactc
      961 aaattcaaag actgtgcggg tatccccaat aaaaaagcac aaataaaatt aaccaagcga
     1021 gccacatgcg aaggtactgc taatgacggt gcggggttcg caaatggatc cacagccgca
     1081 gataaagcaa gtgccgtcgc cgttgaagtc tggagcaccg aaactccggc aacagggagc
     1141 gcaacacaat ttagttgtgt gacaccagca tctcaagagg tcactatctc caatgcagcc
     1201 aacgcggttg tttattatcc gatgagtgct cgactggtcg tcgaaaaaaa taaaaccgta
     1261 agcaatgtca ctgcgggtaa attttctgca cctgccacat ttacagtaac ctataactaa
     1321 cagactgaca gatccaggaa ctatatatgc atcccactca acgtaagctg atgaagagaa
     1381 taattctgtt tctgtcacta cagtttccca ttacctatcc agccatcgct gggcaggata
     1441 ttgaccttgt tgccaatgta aaaaacagca cttgcaaaag cggaatcagt aaccagggta
     1501 atattgatct tggcgtcgtt ggggtgggat atttttcaga taatgttact cctgaaagct
     1561 atcaaccagg tggaaaagag ttcactgtca cagtatcaga ctgtgcatta cagggaactg
     1621 gcgatgtgct taatcagtta catattgatt ttagagccct tagcggtgtc atggctgcag
     1681 gttcaaggca aatatttgcc aatgaagtcg caaccggggc taaaaatgtc ggtgtcgttc
     1741 tcttttccat tcaggacccg accaatatat tcaatgttat tagttcagca ggaaattctc
     1801 gttccgtgta tccagtaatg acttctgccc tgcataattc ttcatggaaa ttttatgcca
     1861 gaatgcaaaa aatcgatccc gcgctggatg tcatatctgg ccaggtaatg agtcatatat
     1921 tagttgatgt gtactacgaa taatagatca aaacaaataa ctgcgactgt tacagcagta
     1981 gatggtggag tgataatgaa gaaattcttc agacattttc tttttttaat tctgtgcttg
     2041 agttgttata ccgcgagtgc tggcactgat gataacgtag gatatattgt tgggaatagt
     2101 tacggcgttg gtccgtcaga tcaaaaatgg agagaaacgg ggccgaatgg cgatgctact
     2161 gtcatattca gatatgcaac aagtacaaat aatttagttt tctacaaacc gacgcagctt
     2221 ggacctacag gtgtaaaatt acagtggagt cagttagata ccgcttctgg tggtggtttt
     2281 ctttattgca acagaagtga tagcacaagt ggtagcgcaa tgcgtattga aaatgcaatg
     2341 gttgactcag gtaaaatgta tggctcccat aaattattta atacatcagt tcctggtttg
     2401 tattacacat tattaatttc aaacatgtgg tcagcttacg gtaccgtaac taacgttagt
     2461 tcacctggga tatacattgg tgactctgca gaacaatatt tttcgtggta taatccaagc
     2521 gaagacgtgt tatattggag ttgcaataat gcgaatagca cccgtaaata ctgggctgta
     2581 ggtggtattt atcagaccct tacaattgaa ttctatacag atacaaactt tgatccaaca
     2641 gtaactcaac agatcaaatt atccagctca tcaaattatc tgtattcatt taaagcctat
     2701 ggcgcaggcc aaggtataaa tgagcatagt tattttatca aaatcgattt cgatctgctc
     2761 aatgtcaagt tgactaatcc cacttgtttt accgctatgc ttagtggaac ttctgtgact
     2821 ggttctacgg taaaaatggg tgaatatagt gcagaacaaa tcagaaacgg tgccacaccg
     2881 gttccttttg atatttcact tcaaaactgc gttcgtgtga ctaatattga gacaaaatta
     2941 gtttcaacaa aggttggtac tgaaaacggg caactccttg gtaatactct cacaggtaat
     3001 gacgcagcaa aaggagtcgg tgtactcata gaaggtttag caactagtaa aaatcctcta
     3061 atgacattga aacctaatga ttcaaattct gtttataaag attacgaccc gagaggcaaa
     3121 gacgatacaa caggaggagt ttacccggat caagatacag gtataacata ccctctccat
     3181 ttccaggcca cgctacaaca ggatggaact ataccaatag aagctggtga gtttaaagcc
     3241 accagtactt tccaggtaac ctacccttaa taagtccacc gcaccgccat catctggcgg
     3301 tgcggtaatt gataaatcat tgcccccacc aatgtgactg ataaaatgcc aggcaacacg
     3361 acatcattta tcaggacacg ttatgaaacc gaccaccatt gcttcacttc agaaatgtaa
     3421 gcaggataaa aagcgtttcg cgaccatcac cgcttacgac tacagcttcg cgaaactatt
     3481 tgctgatgaa ggacttaacg tcatgctggt aggcgattcg ctgggcatga cggttcaggg
     3541 ccacgactcc acccttcccg ttaccgtcga ggatatcgcc tatcataccg ccgccgtacg
     3601 tcgcggcgcg ccaaactgcc tgctgttggc tgacctgccg tttatggcgt atgccacgcc
     3661 ggaacaagcc tttgaaaacg ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat
     3721 tgagggcggt gagtggctgg tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt
     3781 atgtggtcac ttaggtttaa ccccacagtc agtgaatatt ttcggtggct acaaagttca
     3841 ggggcgcggc gatgaagcgg gcgatcaact gctcagcgat gcattagcct tagaagccgc
     3901 tggggcacag ctgctggtgc tggaatgcgt gccggttgaa ctggcaaaac gtattaccga
     3961 agcactggcg atcccggtta ttggcattgg cgcaggcaac gtcactgacg ggcagatcct
     4021 cgtgatgcac gacgccttcg gcattaccgg cggtcacatt cctaaattcg ctaaaaattt
     4081 cctcgccgaa acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc
     4141 cggcgtttat ccgggcgaag aacacagttt ccattaagga gtcacgttgt gttaattatc
     4201 gaaaccctgc cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg
     4261 gcgctggtgc ctactatggg taatctgcac gatggccata tgaagctggt cgacgaagcc
     4321 aaagcccgcg ccgatgtggt cgtcgtcagt attttcgtta acccgatgca gttcgaccgc
     4381 ccggaagatc tggcacgtta tccacggacc ttgcaggagg actgcgagaa gctaaacaaa
     4441 cgtaaagtgg atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtactgaa
     4501 acccatactt acgttgacgt tcccgggctt tcgaccatgc tggaaggcgc gagccgtccg
     4561 gggcattttc gcggcgtttc aactatcgtc agcaagctgt ttaacctggt ccagccggac
     4621 atcgcctgct tcggtgaaaa ggactttcag caactggcgc tgatccgcaa aatggttgcc
     4681 gatatgggct tcgatattga gattgtcggt gtgccaatta tgcgcgccaa agatggcctt
     4741 gcgctcagtt cccgtaatgg ttatctgacg gcggaacaac gcaaaattgc gcccggtctg
     4801 tacaaagttt taagttcaat tgccgacaaa ttgcaggctg gggaacggga tctcgatgaa
     4861 attattacca ttgcggggca agaactgaat gaaaaaggct tccgcgccga tgatattcag
     4921 attcgcgatg ccgacacatt gctggaagtt tctgaaacca gcaaacgggc agtaattctg
     4981 gtagccgcct ggcttggcga tgctcgcctg atcgacaaca aaatggtcga gctggcgtaa
     5041 tacttaactg gcgctacggc tgatggcgcc agttattaat ttaccccacg tcatctgtca
     5101 taacacaggt aacttaaata aggttaatta ccttatcaat ctcagcaaga ggtaaatttg
     5161 ccatctctgc aacgtcttcc cgagacattc ctttacttag aagacgcagg gcgaattctt
     5221 gcctttcctc ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact
     5281 gcgccagcgt catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca
     5341 aatccgcttg ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac
     5401 ttccgctagt gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt
     5461 cgcgctggcg aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga
     5521 tttcgtcatc cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc
     5581 gtgccagctc cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg
     5641 cctcgccctg ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca
     5701 gatgccggtg catggcagca atagaataac gcatcatgcg aaaggccatt ttcttatccg
     5761 gcttgctttg gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg
     5821 aatagagcac gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta
     5881 aatgaagcgt gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa
     5941 agtcgcgagc cgtctccgca tgcattaaaa attgttaaat accgcgtcat gcggtgtggt
     6001 actcggtgca tccatcttcc ctgtcttccg tgttgtcttg cgtgaaggca gtatccagtc
     6061 aaggcgcgcc gtcgtcagcc gcctctttag tgtttaccga gcagcgttca gagaaatttc
     6121 aattcgttac aggcgataca tggcacgctt cggcgcggta cgcaaactgc tgacgcaaat
     6181 gccatcagat gagtagacac tcatcaaaaa tcgggcaata ctgcgtgaga atttcctcaa
     6241 agcaggcacc gcctgcttcg ttaacgacag ggtagaaagg tagaagttat gattcgcacg
     6301 atgctgcagg gcaaactcca ccgcgtgaaa gtgactcatg cggacctgca ctatgaaggt
     6361 tcttgcgcca ttgaccagga ttttcttgac gcagccggta ttctcgaaaa cgaagccatc
     6421 gatatctgga atgtcaccaa cggcaagcgt ttctccactt atgccatcgc ggcagaacgc
     6481 ggttcgagaa ttatttctgt taacggtgcg gcggcccact gcgccagcgt cggcgatatt
     6541 gtcatcatcg ccagcttcgt taccatgcca gatgaagaag ctcgcacctg gcgacccaat
     6601 gtcgcctatt ttgaaggcga caatgaaatg aaacgtaccg cgaaagcgat tccggtacag
     6661 gttgcttgat tgttatccct gcggctggtt actcaccagc cgcgacatcg tctccagcga
     6721 atccgttctt aagatataaa gtcgttttag taacaacgga ttatcccccg gttttacttt
     6781 gcctttcatg gttgtcaccg ccaggtgaaa tccggcatcg tttgctgcct tcacggcttt
     6841 gtcattaaat ccgccaaacg gatacgaaag ataccagaca tgcggattaa attgcgccag
     6901 agcgcggcgt gaacgtgcaa aatcaaacag aatattgtgc tcactacggc tcagtaatat
     6961 gggtcggcga tagccatcca cccgatgcaa aaaatgggta tgtgactgaa aatcaaaaac
     7021 atcgcgaatt tcgttaagct cagaaacgct cataaattgc agcgattttg ggttccactt
     7081 ctgcgggtga cgtttgatgc gcgaggtaac aacaaacgcc gtcgccttca tgccatattg
     7141 tttcaataca ggatacgcat agcggctcac cgacttgagg ccatcatcaa aggtaatcac
     7201 caccgctcgc gcagggagat tgatcttatt cttcacgtaa ccttccagct gcgccatgct
     7261 cagtgtcgcg tatcccctgt cgcgcagcca ggccatctgg ttattgaaag cgcgtaccga
     7321 ggtggtcgtc gaagtatggc gaaaacgggt gttttcttca tcgcgcagaa tatggtgata
     7381 ggttagcacc ggcaggccat tatcgggttg ggcatccagc gcgctgatat aggcgagtcg
     7441 atcgccaata cggatctgat accaggtttg atttaacctg tctttcagtt tatgcaaaat
     7501 cgggtagcgc aaattgtccg ccagtacccc aaatggcgca ctgcccgcac tcggcgcgtt
     7561 atagaccggc gtatctttcc aggtaattaa gttctgatta ctcagcggct tgttgaggtc
     7621 acccaaaccg tcttcaactt tttgtcgccc ctgaactggc tcgagatgcc ctttatcgat
     7681 aaacccttta ccaaagccaa aattaaatgc gtaataactt gcggcagtgg gctccacggc
     7741 aatgatttgc ccggcccgaa tattccccac ggtcaccatc ttgtcgccaa tttgcgccca
     7801 gatcgcagca ttttcgatgg tttgcatata acgggcaggt aacgcggcac tgacacttgc
     7861 ggtaaacagc atcagcagga gaataacagc ttgtttgtac atgaaaaaat aaccgagggt
     7921 gaaacaacgc ttttattgta gcaaaagcgc tataagaatc gtgggttaat actcatacaa
     7981 attatgtttg aggacgaaag gcggattttt ttgttgttgg tgccaaagac tactcacctc
     8041 cggcccaccc agttcgacaa tacgctcgcg aaactctgaa ctagtcatgg tttcacggca
     8101 agccatcatc tgttcaatta acggtaacgt gacaccagaa atcacttcgc aacgggagtg
     8161 tttgtggctt aataatgaag ccacgcggta cggtggcgct ccggctatat cggttaagaa
     8221 gatgacaccc tcaccagagt ccgcttcatg cagagcatcg cacatcatgc ggctgagcat
     8281 attagagctt aacccacgcc agaaattcac ggcccggcac tgaagaagcg ccccatgttt
     8341 tttctccagc gcatccagta tctcttgcgc ccggtcatcg tgacaggtaa ttacccaacc
     8401 taacatcgcc ttcctcctta gccgaaaatc agtctaccct gaccattgtt gataaagggt
     8461 gatagccgtc aaaactcctc tcccccaaat ccagaggaga ggaaatagcc ttagctacgc
     8521 aaaccacgtc cacgttggat cagcgaccaa cagatcaaat aaaacgccac aataaagacc
     8581 accagtacgc caaaggtagt gaccagcgga acatcattga taccgaggaa gccgtagcgg
     8641 aaaccactga tcatataaac gattgggttc aggtgcgaca gcccttgcca gaacggcggc
     8701 aacaaagtca gtgagtaaaa gaccccaccc aaatacgtga gtggcgttaa cacaaaggtt
     8761 ggcaccaggc tgatgtcatc gaacgttttg gcaaacacac cgttcagcaa acccgcaagg
     8821 gagaacaaca ccgccgtgag caccagggtt aaggcaacga atacccacga atgcacctga
     8881 aacggcacaa aaaacagtga aattgccgtc accagaatgc caacaaacag accacgcgcc
     8941 acgccaccgc cgacatatcc ggcaataatg acgtgagtcg gaaccggcgc taccagcagc
     9001 tcttcaatat tacgctggaa cttggcacca aaaaatgatg acgcaacgtt ggcgtaggca
     9061 ttggtgatca ccgacatcat gatcagcccc ggtacgatga actgcatata gctgaagcca
     9121 tgcatatcgc caatacgcga accaatcagg ttaccgaaga taataaagta aagggtcatg
     9181 gtgatgactg gcggcaccag cgtctgcacc cagatacgca taaagcgatg gatctctttc
     9241 gcccagatgc tttttagcgc cacccagtaa agatgcatca tgcgcgatct ccttgttttt
     9301 cattaaccag tgaaacaaac agctcttcca gacggttagc tttgttacgc atacttaata
     9361 cctgaatgcc ctgctcactt aactgcgtaa atacgctgtt gatcccctgc tcacgcagca
     9421 cttcaacttc cagcgtcgcg gtatcgacca gtcgatactg atagccatcg agcttcggta
     9481 acgggctttt cggtgcgaga tcgagaataa aggtttccga tttcagcttc gccagcagcg
     9541 ccttcatcga ggtattttcc accagctcac cgtgttgaat aatgccgata ttgcggcaca
     9601 gcatttctgc ttcttccagg tagtgtgtgg tgagaatgat ggtggtgcct ttgtcgttta
     9661 aatccttcaa aaagccccac attgagcggc gaagttcaat atccacgcct gcggtcggtt
     9721 cgtcgagaat cagtagttta ggttcatgca ttaacgcacg ggcaatcatt aaacggcgct
     9781 tcatcccgcc agataacata cgcgcacgtt cgttgcgttt tccccataga tcgagttgtt
     9841 taagatactt ttcgctgcgg atgtacgctt ctttgcgctc cacgccatag taccctgcct
     9901 gattcaccac aatttgctgc acggtttcaa acgggttgaa gttaaattcc tgcggcacca
     9961 gtcccaactg acgtttagcg ttcacgacat ccttctcgag atcgtaacca aatacgctga
    10021 cccgcccgga ggttttattt accagagagc tgataatacc gatagtggtc gatttcccgg
    10081 ccccgttcgg cccgagaagc gcataaaaat cacccgcttc gacctgcaaa tctatcccac
    10141 gaagcgcctg aacgccgcct ggataggttt ttttaagctg ttgaagttcc agtgcaatgg
    10201 tcataaattt ttacttacct tacgttctta cactttatct gtggtttaaa tcgtcccgga
    10261 gttgccctat attagccaaa cgtaattatt tggttacagg tcgttaacct ccatgaaaga
    10321 catagataca ctcatcagca acaatgcact atggtcaaaa atgctggtgg aagaggatcc
    10381 cgggtttttt gagaaactgg cacaagcgca aaaaccgcgc tttctatgga ttggatgttc
    10441 cgacagtcgc gttcctgcag aacgtttaac cggtcttgag ccgggcgaac tctttgttca
    10501 ccgtaatgtt gctaacctgg tcattcacac tgacctgaac tgcctttccg tggttcagta
    10561 tgcagtggat gtactcgaag ttgaacacat tattatctgt ggccactacg gttgcggcgg
    10621 cgtacaagcc gcagttgaaa acccggaact ggggcttatc aacaactggc tgctgcatat
    10681 ccgcgatatc tggttcaaac atagctcatt gctcggcgaa atgccgcaag agcgccgtct
    10741 ggataccttg tgtgaactga acgtcatgga acaggtgtat aacctgggcc actccaccat
    10801 tatgcaatca gcgtggaaac gcgggcagaa agttaccatt cacggctggg cctacggcat
    10861 tcacgacggc ttgctgcgtg atctggatgt cacagcgacc aaccgcgaaa cccttgagca
    10921 acgttaccgt cacgggattt ccaacctcaa gctgaaacac gccaaccaca aataaaaatg
    10981 ccatgccgga tgcaacacat ccggcaactt cacacttact cgtccagcag aatcactttg
    11041 ccgatatacg gcagatgacg gtaacgctgt gcgtaatcaa tgccgtaacc caccacaaac
    11101 tcatccggga tcgagaaacc gataaattct accgggacgt tcacttcacg acgggacggt
    11161 ttatccagca gcgtacaaat cgccagcgac ttcggttcgc gcaggcttaa gatctcacgc
    11221 actttcgaca gtgtattccc cgagtcgatg atatcttcaa caatcagcac gtccttgcca
    11281 cggatatctt catccagatc tttgaggatt ttcacatcac gggtggtgga catgccgcta
    11341 ccgtagctgg aggcggtcat aaagtcgact tcatgagata cctgaacttc acggcacagg
    11401 tccgccataa acataaatga gccacgcagc agaccaacca gcaccatatc gctgccgctg
    11461 tctttgtaac gctcagtaat ctgacgaccc agttcggcga tacgcgcttt aatctccgct
    11521 tcggggatca ttacttctac agtatgtttc atatctctaa ccatatgatt taaaaacaaa
    11581 tcacttcgca cgggcaggtt ttggcaccgc aacaaagcgc aagacacgca gtataccagt
    11641 aaaactattc attcgcggaa tctgttaata aagctcgaat tgtgatgacg atcacacatg
    11701 ttaaaaccta taattaattg ctactaaaat attaatgaat tgaaatggtg tctctttatg
    11761 gcaattaaca atacaggctc gcgacgatta ctcgtcacgc taacagccct ttttgcagcg
    11821 ctttgcgggc tgtatctact cattggcgga ggctggctgg tcgcgattgg cggctcctgg
    11881 tactacccta tcgctggtct tgtgatgctc ggcgtcgcct ggatgctgtg gcgcagtaaa
    11941 cgtgccgcgc tttggctgta cgcagccctg ctgctcggca ccatgatttg gggcgtctgg
    12001 gaagttggtt tcgacttctg ggcgctgact ccgcgcagcg acattctggt cttcttcggc
    12061 atctggctga tcctgccgtt tgtctggcgt cgcctggtca ttcctgccag cggcgcagtt
    12121 gccgcactgg tggtggcact gctgattagc ggtggtatcc tgacctgggc cggatttaac
    12181 gatccgcagg aaatcaacgg caccttaagc gccaatgcca cacctgcgga agctatctca
    12241 cccgttgccg atcaggactg gcctgcctat ggtcgcaatc aggaaggtca acgcttttca
    12301 cctctgaaac aaattaacgc cgataacgtc cataatctga aagaagcctg ggtgttccgt
    12361 actggcgatg tgaagcagcc gaacgatccg ggcgaaatca ccaatgaagt gacgccgatt
    12421 aaagtgggcg acacccttta cctgtgtacc gctcaccagc gcctgtttgc gctcgatgcc
    12481 gccagcggca aagagaaatg gcattacgat cctgagctga aaactaacga gtctttccag
    12541 cacgtaacct gccgtggcgt ctcttatcat gaagccaaag cagaaacggc ttcgccggaa
    12601 gtgatggcgg attgcccgcg tcgtatcatt cttccggtca atgatggtcg actgattgcg
    12661 attaacgctg aaaacggcaa actgtgcgaa accttcgcca ataaaggcgt gctcaatctg
    12721 caaagcaata tgccagacac caaaccgggt ctgtatgaac cgacttcgcc accgattatc
    12781 accgataaaa ccatcgtgat ggccggttca gttaccgata acttctcaac ccgcgaaacg
    12841 tctggcgtga tccgtggttt tgatgtcaac accggggagc tgctgtgggc ttttgatccc
    12901 ggcgcgaaag atccgaacgc aatcccgtct gacgaacaca cctttacctt taactcgcca
    12961 aactcctggg caccagcggc ctatgacgcg aagctggatc tggtctatct gccgatgggc
    13021 gtgaccacgc cggatatctg gggcggtaac cgcacaccgg aacaggaacg ttatgccagc
    13081 tcgattctgg cgctgaatgc cactaccggg aaactggcgt ggagctacca gaccgttcac
    13141 cacgacctgt gggacatgga tcttccggca cagccgacgc tggcggacat caccgttaat
    13201 ggtcagaaag tgccagttat ttacgctccg gcgaaaaccg gcaacatttt tgtgctcgat
    13261 cgtcgcaatg gcgaactggt ggttccggcc ccggaaaaac cggtgccgca aggtgctgcg
    13321 aaaggcgatt acgttactcc gacccagccg ttctctgaac tgagcttccg tccgaagaaa
    13381 gatttgagcg gtgcggatat gtggggagcc accatgtttg accaactggt gtgccgcgtg
    13441 atgttccacc agttgcgcta tgaaggcatt ttcacgccgc catctgaaca gggcacgctg
    13501 gtcttcccgg gtaacctggg aatgtttgaa tggggcggga tttccgttga tccaaatcgt
    13561 gaagtggcaa ttgccaaccc aatggcactg ccgtttgttt cgaaactgat cccacgcggt
    13621 ccgggtaacc cgatggagca gccgaaagat gccaaaggca cgggtacgga atccggcatt
    13681 cagccacagt acggtgtacc gtatggtgtc acgctcaacc cgttcctctc accgtttggt
    13741 ctgccgtgta aacagccagc atggggttat atctcggcgc tggacctgaa aaccaatgaa
    13801 gtggtgtgga agaaacgtat tggtacgccg caggacagta tgccgttccc gatgcctgta
    13861 ccggtgccgt tcaatatggg tatgccgatg ctgggcgggc caatctccac ggcgggtaac
    13921 gtgctgttta tcgccgctac ggcagataac tacctgcgcg cttacaacat gagcaacggt
    13981 gaaaaactgt ggcagggtcg tttaccagcg ggtggtcagg ctacgccaat gacctatgaa
    14041 gtgaatggta agcagtatgt ggtgatctcc gcaggcggtc acggttcatt tggtacgaag
    14101 atgggcgact atattgtggc ttatgcgctg ccggatgatg tgaagtaacc ataatgcccg
    14161 gagaaatccg ggcatatttc cgaatacggt ctttttatac cgtaaaccct aacatcatcc
    14221 ccgtatcttc atgctccagc agatggcagt gcgccatata agcatgttct ttcggtgcgt
    14281 cgtgattaaa cttcaccagc acttcactga cattgccttc taccttaacg gtatctttcc
    14341 agcccgcgcg atgagccgct ggcggtttgc cattttctga caagatacgg aactgggtgc
    14401 cgtgaatatg gaacggatgc agcatcatgt cgcccacgcc agagataacc caacgttcgt
    14461 actgcccttt cgccgccgca aacatcggct tattcatatc aaacgcctga ccattgattt
    14521 tattggcatg gtggaaatcg aacttcccgc cgtggttcat atgattcata ttaccgtgcc
    14581 ccatatggcc catcatctgg ctgtgatcca ttccagccat cgcctgatcg ccatatttct
    14641 ccattagcat ctgcataccc atcatatcga gcatcgggtc catagaaagt tgcagcttgc
    14701 gtaccgtcag cccttccagc gaaggtaacg caggcaggct acttaatgtg tctggcaaag
    14761 caccggaggc actaatagca atcggctgaa tccgcattac cggatgcggc ttgtcaaacg
    14821 gcgcaatcgc catccccatc tggctgaccg gtagcgtcac caggtcaaag ggtttgttgt
    14881 cgttaacctc caccagcact tcaaaacgct cgcccatcag caccggcagc tcgctcacct
    14941 tcaccggttc aggtagcaga ccaccgtcgc tggcaatcac atacagcggg cgattgtcgc
    15001 tggtggcgaa attgagcgag cgggcattac agccattgag caaacgcagg cgcagccaac
    15061 cacgcggggc agcgtgttgt gggtagattg caccgttggt cagcagcgta tcgccaaacc
    15121 agcccacggc ggcggtcatc acatccagtt gataatcaat ctgcccatca gcgttaaatt
    15181 ttttatcctg aacaatcacc ggaacatcat cgatacccca ctgttttggc agcattaatt
    15241 tcaggatctc gtcatcttca attaccacca gcccagccaa ccccatcgcc acctgtcgcc
    15301 cggtcttgcc atgttgatgt ggatggaacc agcaagtagc ggcaggttga tcaacgttca
    15361 acgtcaccga gcgcttgcca cctggcagaa taattccctg cgggccgccg tcgacttcac
    15421 ccggtacttc cagcccgtgc cagtgcaacg tcgtctcttc cgtcagttgg ttatagatat
    15481 caaccgttac cgctttgccg cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat
    15541 agccccaggt agttgcagtt ttctcgccaa aggtagactg acctgcgcca atagttaact
    15601 gaatgcgatt acgggcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttccg
    15661 ccgcaaagac tgcgcggctc cacagcggca aggctgaagc cacacccagc gcgacggaat
    15721 attttaagaa atcacgacgt tgcatagtta tttccttatt cttaagcagg cgaaacaaaa
    15781 tcaaacgttg agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat
    15841 cgacaataat ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct
    15901 ggtagaagca atgaagacgt ttttcagaac agtgttattc ggcagcctga tggccgtttg
    15961 cgcaaacagt tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt
    16021 ttttgtcttt ctgaagaacg attgtggtta ccagaactta cctaacgggc aaattcgtcg
    16081 cgcactggtc tttttcgccc agcaaaacca gtgggatctc agtaattacg acaccttcga
    16141 catgaaagcc ctcggtgaag acagctaccg cgatctcagc ggcattggca ttcccgtcgc
    16201 taaaaagtgc aaagccctgg cccgcgattc cttaagcctg cttgcctacg tcaaataatc
    16261 cctgatacct ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt
    16321 tgcgcccttt ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca
    16381 gtggcatgaa acgctacacg accagtttgg gcagtacttt gcggtagata acgttctgta
    16441 tcatgaaaag accgatcacc aagatctgat catttttgag aacgctgcat ttggtcgcgt
    16501 aatggcgctg gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat
    16561 gatgacccat gttccgctac tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg
    16621 cggcgacggt gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat
    16681 ggtggaaatc gatgcgggtg tcgtgtcgtt ctgccgtcag tatctgccca accataacgc
    16741 cggtagctac gacgatccgc gctttaagct ggtgatcgac gatggcgtca atttcgttaa
    16801 tcaaaccagc cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg
    16861 cgaaagtctg ttcacctcag cattttatga aggctgcaaa cgttgcctga atcctggcgg
    16921 tatcttcgtc gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcca
    16981 tcgcaaactc agccattact tcagcgacgt tggcttttat caggcggcga tcccgaccta
    17041 ttacggcggt atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc
    17101 aaccgaaatt attcaggcgc gttttctcgc ttctggcctg aaatgccgtt attacaatcc
    17161 ggcaatccat acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca
    17221 gccgtcctaa ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct
    17281 gaccaaaagt ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg
    17341 cgacggttat attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct
    17401 gtcagaaacc tgttccatta tcggggccaa tattcttaac atcgcccgcc aggattacga
    17461 accacagggt gccagcgtca ctattctggt gagtgaagaa ccggttgacc cgaaactcat
    17521 cgacaaaaca gaacaccccg gcccactgcc agaaacggtg gttgcccatc tcgataaaag
    17581 tcatatttgc gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg
    17641 cgccgatatt gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct
    17701 gatccaccag cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttactcg
    17761 cgacattaac ggtatgaagc actttatcga ccatgagatt aattcgattc agaactttat
    17821 gtctgacgat atgaaggcgc tgtatgacat ggtggatgtg aacgtctatc aggaaaatat
    17881 cttccatacc aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa
    17941 accggaagac ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagagat
    18001 gcgcgagatt tattacgggc gcaatatgcc agctgtttaa cggctctggc ggagctccca
    18061 ggctccgcca gatttattta cttctgctgc acgaaattac ggtaagccgc cacgacctgc
    18121 agaaaatcct caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccctct
    18181 tccatttcat cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc ttcaccgtcc
    18241 atccacagag tgtactcatg ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg
    18301 tgcgctgctt gttccacttc atcaagcaag gccaggtttt ctttcacctc ttcattaaac
    18361 cagtgcccga ccacttcatg ccccatggac atacgcacct ttaccactcc ggtaatatcg
    18421 cgcagaaatt cgtaatccat agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat
    18481 tgcgccattg atgtaattat cgcagcctta ccggagaaaa acagcggaac ggcgatggtt
    18541 tagaaataaa aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc
    18601 gcgtgcgctt acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta
    18661 ctgagaaagc tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg
    18721 cgccacgtag gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc
    18781 cgccagttcc gcagaagcca ggaagacatt cgcgccagta cccagacggt tcgggaagtt
    18841 acgggtagag gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca
    18901 cagggaacag ccagggatct cgatacgcgc accactctta ccgaagacgc tgtagtagcc
    18961 ttcttcagtc aactgcgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg
    19021 cagttggcct ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttggt
    19081 catgcaggaa ccgataaata cttcgtcgat cttctcaccc tgtaccgcag acagcggacg
    19141 cgcgtcatcc gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat
    19201 gtcgatcact gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag
    19261 ccatttttcc atgccctgaa tacgacgttc cagggtgcga cgatcgccgt aaccttccgc
    19321 gatcatccac ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt
    19381 cagcttgatg gtacaaccag cggcagaacg ctcggcggac gcatcggtca gttcaaatgc
    19441 ctgctcaact ttcagatccg gcagaccttc aatttccagg atgcggccag agaagatgtt
    19501 tttcttgcct ttcttctcaa cggtcagcag accttgtttg atcgcataca gcggaatggc
    19561 atgtaccaga tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac
    19621 ggattccggc atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga
    19681 acccgccggg aaagagatac cgatcgggaa acgggtatgg gaatcaccac cggtaccgac
    19741 ggtatccggc agcagcatac ggttcagcca ggagtgaatg acgccgtcac ccggacgcag
    19801 cgacacaccg ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac
    19861 tggcttcgga tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc
    19921 caggcacgcc aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgggaacc
    19981 tacagaagtc attttcggtt cacagtacgc gcccggacga atgcctttca cgccacaggc
    20041 acggcctacc attttttgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc
    20101 ctgacggaac acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc
    20161 acgcccgata atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt
    20221 tttcagttcg aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta
    20281 cgggtaaacg tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag
    20341 tgcacccgcg tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa
    20401 accaccgccg cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga
    20461 gttagtggcg gatttacgcg aagaacccgt acccacaacg tcaccgacgt acgccagcgg
    20521 gaaacctttc tgttgcagag cttcgatttg cttgatcgga ccaacaacac caggctggtc
    20581 tggctcaata ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg
    20641 gcgtgaccac gcatccggtg ccggagaaag gtcatcggtg ttagtttcgc cagtgacttt
    20701 gaagacggta acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc
    20761 cgcccaggac tgcataacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac
    20821 gtcatagaag ttatcgaaca tcagcagcgt gtgagaaagt gctttggcag caataggtgc
    20881 cagtttggca tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt
    20941 gcccagcagt tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttcgc
    21001 gatagcagcc aggaagcctg ctttgacata ggcggcttca tcgacgcctg ggggaacacg
    21061 gttggttaac agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc
    21121 tacaagtgcg gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg
    21181 ctcagctacg tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat
    21241 agtgcggcag gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc
    21301 agtaccgcaa cgggcagaat agcaggattt tgacgatgtg ttaatctgtt tacaaaaaag
    21361 caacataaaa aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa
    21421 acttcgttaa ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa
    21481 tttaaactac cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac
    21541 cattgcgatt tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg
    21601 ctggcgctaa tttgcagtgc cgggctttgt gccgcctcta ccgggctata tataaaaagc
    21661 cgcacagtgg aagcgcctgt ggaaccgcaa tcgacacaac agactgcgcc tgacatcacc
    21721 gcagttacgc ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc
    21781 gcattcagca ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct
    21841 ctgctttcac aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtacaa
    21901 tggtcgcacg ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag
    21961 ccgtgggacg ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc
    22021 gaaaacccgc aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg
    22081 atggactcgg tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca
    22141 accgaacaga aagttattac cacaacgaag aaagctgtac cggtaacaca gacagtcacg
    22201 gctcccgtca taccatccaa taccgtttca actgccagcc ccgtcattac agagcctgca
    22261 acaaccgtca tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac
    22321 ccaaccgtgg tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca
    22381 ccagccggag aaccgtttat tgacagcttt gtgcgcggat tcggctatag catgggtgtt
    22441 gctaccacgt acgcactatt cagcagcatc gactgggacg acgacgatca tgaccatcat
    22501 catcatgacg atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg
    22561 caacacaacg gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag
    22621 catcttactg ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc
    22681 tatcatgatc aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagc
    22741 gccacgcagc tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt
    22801 cagcaacgaa cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca
    22861 cagcggttta atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt
    22921 cgccaacccc tgacccagca acaaaaggac gccgctcgtc agcgttatca gtccgcctcg
    22981 cctgagcagc gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga
    23041 agagaggcag cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtatttaag
    23101 gaaaaagtac agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata
    23161 caatcggcat cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca
    23221 caacgtctaa acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcggcagta
    23281 cgcgaacgtc tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa
    23341 aaagcggcgt ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aacaattact
    23401 tcttcttcgc tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca
    23461 ggcccacaga ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat
    23521 cacaacccat ttcgattgcc aggccgattt cacccagcag ctcgccgccg ttggtaccga
    23581 caatcgcacc accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt
    23641 ctgcgcagtc ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat
    23701 agctgatgcc tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt
    23761 cggtataggc gatggacggg ataactttcg gatcgaagta gtgtttctta ccggcgataa
    23821 cttcagcggc aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccgacga
    23881 tatcgccgat agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga
    23941 aaccacggtc gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca
    24001 cacgaccaat cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc
    24061 cttccatcgt cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca
    24121 gcatcaggtt gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag
    24181 ctgccgggat aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt
    24241 atacggtgcc catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta
    24301 cttctttcag ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa
    24361 acggcagttg gatcgggcga gaacccgctg caatgatcgc gttgtcgaag ttgatcacgg
    24421 ttttaccgtt ctcaccttca acttccaggg tgttagcccc ggtaaattta cccagaccgt
    24481 tgaccacttt gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga
    24541 tcactttctc tttccaggta cgaatcttgt caatatcggt tttcggttcg ccgaagacga
    24601 taccgtgttc agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt
    24661 tagaagggat acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga
    24721 ttacggtttc cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg
    24781 ccccaagtac cacgacctga gttttgattt cagtactcat catgacctct atatatttat
    24841 ctccggcggt catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc
    24901 ccagtcactt acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca
    24961 gaaatccata gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg
    25021 tattttacaa aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc
    25081 taataacctc aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt
    25141 ttacttacat caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac
    25201 gggcaccatc agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca
    25261 gacgcggcac gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga
    25321 ggatagccac ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga
    25381 tgctggagat ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg
    25441 ctttcttaga aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt
    25501 ctttgaatac cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt
    25561 atttcttcag ggtcagacgc tgaccgtctt ccgacaacga actgttgaag cgaggcatct
    25621 gctcaagagc tgcagcaacg gctttcatga tgaagacaac cggggtgatc ttcacatcca
    25681 gcttacgttt cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat
    25741 cggttttgtc gaagtgagta acatgcggga tcatcaccca gttacggctc aggttcgcac
    25801 cagaaatttt ctggatacgg cccagttcca cttcttcgat ttcaccaaac ttgctgaagt
    25861 ccaccttcgg ccacggcagc atgcctggga taccgccgcc agtcgccgcc ggagctgctt
    25921 ctgcacgttt gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt
    25981 tacggccagt gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga
    26041 tcagcggagt cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt
    26101 tcgcagccgg agctgctgcc ggagcttcag cttttgctgc cggagccggc gctgccgctt
    26161 cctgtttcgc cggagccgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg
    26221 agccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg
    26281 gagccggaac ttccatagaa gctttgtcgc cttctacggt aatcagtgac tgttcagcgg
    26341 caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat
    26401 ccggaacgtt aacttctttc acgccagccg ctggtgcagg ggccgctgcc ggagccgctt
    26461 cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca
    26521 gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa
    26581 acggagccgg aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag
    26641 cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga
    26701 tatccggaac gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt
    26761 ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa
    26821 tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga
    26881 tacccgcctg cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg
    26941 actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat
    27001 cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag
    27061 acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt
    27121 cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc
    27181 atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt
    27241 acccagtacg cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt
    27301 catatagtcg gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac
    27361 gcgcggagtt tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc
    27421 cagctcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag
    27481 gatctctgcc gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt
    27541 acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc
    27601 accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac
    27661 gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc
    27721 gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct
    27781 gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc
    27841 gatcaggaag ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg
    27901 gaagccgaac atcgagtaat agatgtagaa cgggatcatc ggcagattgt tggtgctgta
    27961 agaggtcgcc gctgccagcc aggaacaacc tgcgcccagc tcgttgatcc cttcctgcag
    28021 aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcggggtgta
    28081 ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt
    28141 acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat
    28201 tacgttcagg gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag
    28261 cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg
    28321 gcttggcaga taaccgtgca gtttctgacg ctgtgcatgc agataggtat gctcttcaga
    28381 accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt
    28441 gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat
    28501 gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac
    28561 tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc
    28621 gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc
    28681 ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa
    28741 ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc
    28801 tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt tccagccagc
    28861 accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc
    28921 aagacgctgc aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc
    28981 gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta
    29041 aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc
    29101 ctggtaaata gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc
    29161 cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa
    29221 gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg
    29281 ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa
    29341 gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc
    29401 gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat
    29461 agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac
    29521 ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt
    29581 gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg
    29641 gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg
    29701 gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct
    29761 gtcccattga actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc
    29821 ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat
    29881 tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt
    29941 cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg
    30001 gcttaccggc cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca
    30061 tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct
    30121 ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct
    30181 gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta
    30241 tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt
    30301 aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgactcag
    30361 gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga
    30421 caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg
    30481 cctcacgcaa ggagggacgg gagacgtcaa actgttttgc cagttcgcgt tccggtggga
    30541 gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc tgctcaatca
    30601 catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac
    30661 ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt
    30721 caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt
    30781 gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac gtaggaaagg
    30841 agttttttaa cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt
    30901 tgtgattttt aattcaatta aaacgaattt aaattcattc tacatattga gaggggttga
    30961 ggctgagctt tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagtgg
    31021 gtgcattcgc tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc
    31081 aaacttcttt gatgtaaaca aattaataca acaaacggaa ttgcaaactt acacacgcat
    31141 cactgcgtag atcaaaaaaa caaccaccgc acgaggtttc atgatggaag gtcaacagca
    31201 cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg
    31261 cgcgataggg accgggttat tcctgggtag cgcctccgta atacagtccg cagggccagg
    31321 gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg
    31381 tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg
    31441 gggcagtttt gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc
    31501 catggctgag ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatccccac
    31561 ctgggtttct gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa
    31621 agtgtttggc gagatggagt tctggtttgc cattatcaaa gttatcgcgg tggtagcgat
    31681 gatcatcttc ggcggctggc tgctattcag tggcaacggc ggcccgcagg cgaccgttag
    31741 caacctgtgg gatcagggtg gtttcctgcc gcacggcttc accgggctgg tgatgatgat
    31801 ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc
    31861 tgataacccg gagcaaagta taccgaaagc aactaaccag gttatctacc gcatcctgat
    31921 tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc
    31981 cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc
    32041 gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa
    32101 cagccgtatg ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt
    32161 cgataaacgt ggtgtaccag taaataccat tctggtgtct gcactggtaa cggcgttgtg
    32221 cgtactgatt aactaccttg ccccagagtc cgctttcgga ctgttaatgg cgctggtggt
    32281 atctgcactg gtaatcaact gggcgatgat tagcctggcg catatgaaat tccgtcgcgc
    32341 caagcaggaa caaggcgtgg taactcactt ccctgctctg ctttatccgc tgggtaactg
    32401 gatctgcctg ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc
    32461 gatttcggta tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa
    32521 agagaaaacc gccaaagccg taaaagcgca ttaatctctc tacgccctca cccgtacagg
    32581 gtgagggcaa taatctttac accaacgccc cgtaaatcgt cagtagtgca atcaccacca
    32641 cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cctcggcgtc tccaccttat
    32701 cgacatgcgg ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg
    32761 aagtatggaa atcacccagc gaagcaaacc aggccggtaa cgctttctca ccatgaccga
    32821 tcaaggcata taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg
    32881 catcaatgcc ggactgtaaa cgatgatgcg gcgtctgata tcgcgccagc cagtattgcc
    32941 atgcacgcaa aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca
    33001 gccagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct
    33061 cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc
    33121 cagccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat
    33181 aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagta
    33241 gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca
    33301 tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga
    33361 acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa
    33421 ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcacacgaaa
    33481 ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg
    33541 gcccgtcatg tttttagcga tatccggata gcaatcaatc agtgcccgcg taaccgccgc
    33601 aagctgttga tactgcgcat cggtatacgc cagcgtatcg gtgccttcaa gctcaatccc
    33661 aatagaaaaa tcattgcagc gttcgcgccc ctgatactga gagactcccg catgccatgc
    33721 acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc
    33781 ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc
    33841 agtgaataat acgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg
    33901 caccaccagc agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac
    33961 gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg
    34021 tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt
    34081 tttaacatgc cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc
    34141 attaatctcg atatccccgg cgcggtagcc caggcgctgc gggaagattt aggcggaaca
    34201 gtcgatgcca acaatgatat tacggcaaaa cttttaccgg aaaattctcg ctctcatgcc
    34261 acggtgatca cccgcgagaa tggcgtcttt tgcggcaaac gctgggttga agaggtgttt
    34321 attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc
    34381 aatgccaatc aatccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa
    34441 cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat
    34501 gtcgaattgc tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacccggc
    34561 ctgcgttcag ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg
    34621 ctttctgatg ccttcctgat caaagaaaac catattattg cctccggctc agtgcgccag
    34681 gcggtcgaaa aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat
    34741 ctggaagaac ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc
    34801 gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg
    34861 tctggcaacg tcactgacaa aacactgcgt gaatttgccg aaacgggcgt ggactttatc
    34921 tccgtcggtg cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa
    34981 tagttgacag atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt
    35041 cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc tcggaatctg
    35101 ctttcccgtt tcgttttttg cccctcgcct gtgctgatgg cgtttgccaa agtagcgcca
    35161 actaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat
    35221 ggtggttatt ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta
    35281 cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc gtaccgccgt
    35341 agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat
    35401 tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg gcgtggtgtc
    35461 gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacaccgg gttgggataa
    35521 cgcaaacggc gtcaccggct ggacgcgcaa ctgcaatatt caaagtgaca gcgcattgca
    35581 tcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat
    35641 tccacagctc actgccctgt gtctgcgtta tcatggagtc ttgctggatg ccagcgaaga
    35701 ggtggttcat gttgcggtag tcgatgcacc ttcgcatgag ctactggacg cattgcattt
    35761 cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggtcacgc
    35821 cagtcgcaca caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga
    35881 gttgctaact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata ttcatatcga
    35941 accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc
    36001 ggatgtttca ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct
    36061 ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa
    36121 cgccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag
    36181 gttgttacag caggtgagcc aggcactgga tgtcaacacg cttggaatgc agccgttaca
    36241 actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc
    36301 tacaggcagc ggcaaaacgg tcacgcttta tagtgccctg caaacgctga ataccgctga
    36361 cattaatatt tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac
    36421 gcaaatccat ccgcgtgccg gactcacctt tcagggcgtg ttgcgtgcgt tattgcgcca
    36481 ggatcctgac gtcatcatga tcggagagat ccgcgatggc gaaacagcag agatcgctat
    36541 taaagcggcg caaactggtc acctggtgtt gtctacccta cacactaatt ccacctgcga
    36601 aacgctggta cgtttacagc aaatgggggt cgcccgctgg atgctatcat cggcgcttac
    36661 gctggtaata gcccagcgtc tggtacgcaa actttgccca cattgtcgcc ggcagcaagg
    36721 ggagcccatc cacattccag acaatgtatg gccatcgccg ctgccccact ggcaggcacc
    36781 cggttgtgta cattgctacc acggttttta tggtcgtacg gccttatttg aagttctgcc
    36841 cataacgccg gtcattcgtc agcttatttc cgctaatacc gacgttgaat cgctggaaac
    36901 gcacgcacga caggcgggta tgcgtacgct ttttgaaaac gactgcctgg ccgtggagca
    36961 aggcttaacc acctttgaag agttaatccg cgtactgggg atgccgcatg gcgagtaagc
    37021 aactctggcg ctggcatggc ataaccggcg acggcaatgc gcaagatggg atgctatggg
    37081 cagagagccg tgctttgctg ctcatggcac tacagcaaca gatggttacc ccacttagcc
    37141 tgaagcgaat cgccatcaat tctgcgcagt ggcgaggaga taaaagcgcg gaagtcattc
    37201 atcaactggc gacgctactc aaagccgggt taacgctttc tgaagggctg gcactgctgg
    37261 cggaacagca tcccagtaag caatggcaag cgttgctgca atcgctggcg cacgatctcg
    37321 aacagggcat tgctttttcc aatgccttat taccctggtc agaggtattt ccgccactct
    37381 atcaggcgat gatccgcacg ggtgaactga ccggtaagct ggatgaatgc tgctttgaac
    37441 tggcgcgtca gcaaaaagcc cagcgtcagt tgaccgacaa agtgaaatca gcgttacgtt
    37501 atcccatcat cattttagcg atggcaatca tggtggttgt ggcaatgctg cattttgttc
    37561 tgccggagtt tgccgctatc tataagacct tcaacacccc actaccggca ctaacgcagg
    37621 ggatcatgac gctggcagac tttagtggcg aatggagctg gctgctggtg ttgttcggct
    37681 ttctgctggc gatagccaat aagttgctga tgcgccgacc tacctggctt atcgcgcggc
    37741 agaaattgtt gttacgaatc ccgattatgg gttcactgat gaggggacaa aaactcacgc
    37801 agatctttac gattctggcg ctgacacaaa gtgcaggcat tactttttta cagggcgtag
    37861 agagcgtcag agaaacaatg cgctgcccgt actgggtgca acttctgaca caaatccagc
    37921 acgatatcta tagcgcggcg atttttttga taccgatttg catttcattt gataccgctg
    37981 aaaaacgccg atacgctact tatttgatac taaatcggcg cttttccttt gcgttttaga
    38041 atgagttttt aagcactcac ccaatctctt tcaaaccggt tttaacagtt tgtggttttg
    38101 ggtaaggctg gactttgaat agctttgtgt tgaggcgttt tctggccctc ttgttcagaa
    38161 accggatgta acgaaactgt cggaacttat gaacactggc cctgtcgata ttagccctca
    38221 gatgttcacc tcgttgtccg cctcgtttaa tggcattcct gcaaatctcg tgataccact
    38281 cgccatcaag ttcatagaat gttgattcat ggctgcctac ataatcaaaa ttgcttgcct
    38341 gatacacgac accaagacag ccacaacgct catctgcaaa cgactgaacc cactgtacct
    38401 gcggataaag ctgtctgatt agcttgagcg cgtaactgat tgcccttgat tcggagtttc
    38461 tcggcataca gtcatgcagc catagccggt taagctccat atattctcgg ttctgcgtac
    38521 ccgttacgac gcgtgcacca ctgtttgggt taagggcgta
//
LOCUS       DAZGAN020000037        38514 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGAN020000037 DAZGAN020000000
VERSION     DAZGAN020000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 38514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.037"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            82..744
                     /gene="acrS"
                     /locus_tag="ACHKYO_RS10500"
                     /old_locus_tag="pgaptmp_002100"
                     /pseudo
     CDS             82..744
                     /gene="acrS"
                     /locus_tag="ACHKYO_RS10500"
                     /old_locus_tag="pgaptmp_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
     gene            complement(747..926)
                     /locus_tag="ACHKYO_RS10505"
                     /old_locus_tag="pgaptmp_002101"
     CDS             complement(747..926)
                     /locus_tag="ACHKYO_RS10505"
                     /old_locus_tag="pgaptmp_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HGU4428952.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(1010..1894)
                     /gene="yhdJ"
                     /locus_tag="ACHKYO_RS10510"
                     /old_locus_tag="pgaptmp_002102"
     CDS             complement(1010..1894)
                     /gene="yhdJ"
                     /locus_tag="ACHKYO_RS10510"
                     /old_locus_tag="pgaptmp_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU4428953.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1980..2276)
                     /gene="fis"
                     /locus_tag="ACHKYO_RS10515"
                     /old_locus_tag="pgaptmp_002103"
     CDS             complement(1980..2276)
                     /gene="fis"
                     /locus_tag="ACHKYO_RS10515"
                     /old_locus_tag="pgaptmp_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HGU4428954.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2302..3267)
                     /gene="dusB"
                     /locus_tag="ACHKYO_RS10520"
                     /old_locus_tag="pgaptmp_002104"
     CDS             complement(2302..3267)
                     /gene="dusB"
                     /locus_tag="ACHKYO_RS10520"
                     /old_locus_tag="pgaptmp_002104"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGU4428955.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3596..4477)
                     /gene="prmA"
                     /locus_tag="ACHKYO_RS10525"
                     /old_locus_tag="pgaptmp_002105"
     CDS             complement(3596..4477)
                     /gene="prmA"
                     /locus_tag="ACHKYO_RS10525"
                     /old_locus_tag="pgaptmp_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGU4428956.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4489..5940)
                     /gene="panF"
                     /locus_tag="ACHKYO_RS10530"
                     /old_locus_tag="pgaptmp_002106"
     CDS             complement(4489..5940)
                     /gene="panF"
                     /locus_tag="ACHKYO_RS10530"
                     /old_locus_tag="pgaptmp_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HGU4428957.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5930..6172)
                     /locus_tag="ACHKYO_RS10535"
                     /old_locus_tag="pgaptmp_002107"
     CDS             complement(5930..6172)
                     /locus_tag="ACHKYO_RS10535"
                     /old_locus_tag="pgaptmp_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HGU4428958.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6281..7630)
                     /gene="accC"
                     /locus_tag="ACHKYO_RS10540"
                     /old_locus_tag="pgaptmp_002108"
     CDS             complement(6281..7630)
                     /gene="accC"
                     /locus_tag="ACHKYO_RS10540"
                     /old_locus_tag="pgaptmp_002108"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGU4428959.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7641..8111)
                     /gene="accB"
                     /locus_tag="ACHKYO_RS10545"
                     /old_locus_tag="pgaptmp_002109"
     CDS             complement(7641..8111)
                     /gene="accB"
                     /locus_tag="ACHKYO_RS10545"
                     /old_locus_tag="pgaptmp_002109"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGU4428960.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9089..10063)
                     /locus_tag="ACHKYO_RS10550"
                     /old_locus_tag="pgaptmp_002110"
     CDS             complement(9089..10063)
                     /locus_tag="ACHKYO_RS10550"
                     /old_locus_tag="pgaptmp_002110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HGU4428961.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10215..12155
                     /gene="csrD"
                     /locus_tag="ACHKYO_RS10555"
                     /old_locus_tag="pgaptmp_002111"
     CDS             10215..12155
                     /gene="csrD"
                     /locus_tag="ACHKYO_RS10555"
                     /old_locus_tag="pgaptmp_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HGU4428962.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12460..13503
                     /gene="mreB"
                     /locus_tag="ACHKYO_RS10560"
                     /old_locus_tag="pgaptmp_002112"
     CDS             12460..13503
                     /gene="mreB"
                     /locus_tag="ACHKYO_RS10560"
                     /old_locus_tag="pgaptmp_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HGU4428963.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13569..14672
                     /gene="mreC"
                     /locus_tag="ACHKYO_RS10565"
                     /old_locus_tag="pgaptmp_002113"
     CDS             13569..14672
                     /gene="mreC"
                     /locus_tag="ACHKYO_RS10565"
                     /old_locus_tag="pgaptmp_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGU4428964.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14672..15160
                     /gene="mreD"
                     /locus_tag="ACHKYO_RS10570"
                     /old_locus_tag="pgaptmp_002114"
     CDS             14672..15160
                     /gene="mreD"
                     /locus_tag="ACHKYO_RS10570"
                     /old_locus_tag="pgaptmp_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGU4428965.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15169..15762
                     /gene="yhdE"
                     /locus_tag="ACHKYO_RS10575"
                     /old_locus_tag="pgaptmp_002115"
     CDS             15169..15762
                     /gene="yhdE"
                     /locus_tag="ACHKYO_RS10575"
                     /old_locus_tag="pgaptmp_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HGU4428966.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15752..17221
                     /gene="rng"
                     /locus_tag="ACHKYO_RS10580"
                     /old_locus_tag="pgaptmp_002116"
     CDS             15752..17221
                     /gene="rng"
                     /locus_tag="ACHKYO_RS10580"
                     /old_locus_tag="pgaptmp_002116"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HGU4428967.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17289..21089
                     /gene="yhdP"
                     /locus_tag="ACHKYO_RS10585"
                     /old_locus_tag="pgaptmp_002117"
     CDS             17289..21089
                     /gene="yhdP"
                     /locus_tag="ACHKYO_RS10585"
                     /old_locus_tag="pgaptmp_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HGU4428968.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVEMNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21245..22690
                     /gene="tldD"
                     /locus_tag="ACHKYO_RS10590"
                     /old_locus_tag="pgaptmp_002118"
     CDS             21245..22690
                     /gene="tldD"
                     /locus_tag="ACHKYO_RS10590"
                     /old_locus_tag="pgaptmp_002118"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HGU4428969.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22818..23747)
                     /gene="aaeR"
                     /locus_tag="ACHKYO_RS10595"
                     /old_locus_tag="pgaptmp_002119"
     CDS             complement(22818..23747)
                     /gene="aaeR"
                     /locus_tag="ACHKYO_RS10595"
                     /old_locus_tag="pgaptmp_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HGU4428970.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23930..24133
                     /gene="aaeX"
                     /locus_tag="ACHKYO_RS10600"
                     /old_locus_tag="pgaptmp_002120"
     CDS             23930..24133
                     /gene="aaeX"
                     /locus_tag="ACHKYO_RS10600"
                     /old_locus_tag="pgaptmp_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HGU4428971.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24141..25073
                     /gene="aaeA"
                     /locus_tag="ACHKYO_RS10605"
                     /old_locus_tag="pgaptmp_002121"
     CDS             24141..25073
                     /gene="aaeA"
                     /locus_tag="ACHKYO_RS10605"
                     /old_locus_tag="pgaptmp_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HGU4428972.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25079..27046
                     /gene="aaeB"
                     /locus_tag="ACHKYO_RS10610"
                     /old_locus_tag="pgaptmp_002122"
     CDS             25079..27046
                     /gene="aaeB"
                     /locus_tag="ACHKYO_RS10610"
                     /old_locus_tag="pgaptmp_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HGU4428973.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27138..27410
                     /locus_tag="ACHKYO_RS10615"
                     /old_locus_tag="pgaptmp_002123"
     CDS             27138..27410
                     /locus_tag="ACHKYO_RS10615"
                     /old_locus_tag="pgaptmp_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HGU4428974.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27466..27729)
                     /gene="yhcN"
                     /locus_tag="ACHKYO_RS10620"
                     /old_locus_tag="pgaptmp_002124"
     CDS             complement(27466..27729)
                     /gene="yhcN"
                     /locus_tag="ACHKYO_RS10620"
                     /old_locus_tag="pgaptmp_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HGU4428975.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(28094..28564)
                     /gene="argR"
                     /locus_tag="ACHKYO_RS10625"
                     /old_locus_tag="pgaptmp_002125"
     CDS             complement(28094..28564)
                     /gene="argR"
                     /locus_tag="ACHKYO_RS10625"
                     /old_locus_tag="pgaptmp_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HGU4428976.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28999..29937
                     /gene="mdh"
                     /locus_tag="ACHKYO_RS10630"
                     /old_locus_tag="pgaptmp_002126"
     CDS             28999..29937
                     /gene="mdh"
                     /locus_tag="ACHKYO_RS10630"
                     /old_locus_tag="pgaptmp_002126"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU4428977.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            complement(30000..31067)
                     /gene="degS"
                     /locus_tag="ACHKYO_RS10635"
                     /old_locus_tag="pgaptmp_002127"
     CDS             complement(30000..31067)
                     /gene="degS"
                     /locus_tag="ACHKYO_RS10635"
                     /old_locus_tag="pgaptmp_002127"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HGU4428978.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(31157..32524)
                     /gene="degQ"
                     /locus_tag="ACHKYO_RS10640"
                     /old_locus_tag="pgaptmp_002128"
     CDS             complement(31157..32524)
                     /gene="degQ"
                     /locus_tag="ACHKYO_RS10640"
                     /old_locus_tag="pgaptmp_002128"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HGU4428979.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(32678..33076)
                     /gene="zapG"
                     /locus_tag="ACHKYO_RS10645"
                     /old_locus_tag="pgaptmp_002129"
     CDS             complement(32678..33076)
                     /gene="zapG"
                     /locus_tag="ACHKYO_RS10645"
                     /old_locus_tag="pgaptmp_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HGU4428980.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            33270..34397
                     /gene="zapE"
                     /locus_tag="ACHKYO_RS10650"
                     /old_locus_tag="pgaptmp_002130"
     CDS             33270..34397
                     /gene="zapE"
                     /locus_tag="ACHKYO_RS10650"
                     /old_locus_tag="pgaptmp_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HGU4428981.1"
                     /translation="MQSVTPTSQYLKELNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            34616..35044
                     /gene="rplM"
                     /locus_tag="ACHKYO_RS10655"
                     /old_locus_tag="pgaptmp_002131"
     CDS             34616..35044
                     /gene="rplM"
                     /locus_tag="ACHKYO_RS10655"
                     /old_locus_tag="pgaptmp_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGU4428982.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            35060..35452
                     /gene="rpsI"
                     /locus_tag="ACHKYO_RS10660"
                     /old_locus_tag="pgaptmp_002132"
     CDS             35060..35452
                     /gene="rpsI"
                     /locus_tag="ACHKYO_RS10660"
                     /old_locus_tag="pgaptmp_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGU4428983.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35847..36485
                     /gene="sspA"
                     /locus_tag="ACHKYO_RS10665"
                     /old_locus_tag="pgaptmp_002133"
     CDS             35847..36485
                     /gene="sspA"
                     /locus_tag="ACHKYO_RS10665"
                     /old_locus_tag="pgaptmp_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HGU4428984.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            36491..36988
                     /gene="sspB"
                     /locus_tag="ACHKYO_RS10670"
                     /old_locus_tag="pgaptmp_002134"
     CDS             36491..36988
                     /gene="sspB"
                     /locus_tag="ACHKYO_RS10670"
                     /old_locus_tag="pgaptmp_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HGU4428985.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(37031..38398)
                     /gene="dcuC"
                     /locus_tag="ACHKYO_RS10675"
                     /old_locus_tag="pgaptmp_002135"
     CDS             complement(37031..38398)
                     /gene="dcuC"
                     /locus_tag="ACHKYO_RS10675"
                     /old_locus_tag="pgaptmp_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU4428986.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIHSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMELVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9368 a         9604 c        10106 g         9436 t
ORIGIN      
        1 taaatacaca ttaggtgata gattaacctt cgctattttc tcactctgtg tcgaatatat
       61 ttatttcctg aataattaat catggcaaaa agaaccaaag ccgaagctct gaagacccgg
      121 caagaactga ttgaaactgc catcgcccag tttgcgcagc atggcgtaag caagacgaca
      181 ctcaacgaca ttgccgacgc tgctaacgtt acgcgtggcg ctatctactg gcacttcgaa
      241 aacaagactc aactgtttaa tgagatgtgg ttgcaacagc cttcattgcg ggagttaatc
      301 caggaacact tgacggctgg attagagcat gacccgtttc aacaattgcg tgaaaaattg
      361 attgtcggct tgcaatatat tgccaaaatt ccccgctagc aggcgttgct gaaaatctta
      421 tatcacaaat gtgaatttaa tgatgagatg ctggccgagg gagtgatacg cgaaaagatg
      481 ggcttcaatc cgcagactct ccgcgaagta ttgcaggcgt gtcagcaaca aggttgtgta
      541 gcaaataacc tcgatttaga tgttgtgatg attattatta atggtgcctt cagcggaatt
      601 gttcaaaact ggttaatgaa tatggcgggt tatgatcttt ataaacaagc ccccgctctg
      661 gtcgataacg tattaagaat gttcatgcca gatgaaaaca taacgaaatt aattcatcaa
      721 acgaatgaat taagtgtcat gtaaattcac attctgtcct gaaaattcag ttcgtcacag
      781 ttaaccagtt ttagtggatt aactgagttc atattgcggc atttgtctgt ttctgttgcc
      841 agcagttcaa tccactgcat cagtaaagta tcaaacagaa aaacgaagac agcgaaaacg
      901 acgagccacc aatacttgcg aatcattacc tgagcctgtg tatgtttaac caagtctggc
      961 aggaaatata cacgaaatga ataatctgaa aaccaggctt acacttcctt tactttgcaa
     1021 tgagatcggg gtcaacttcg cttaaccggc ttcgttttga caggttgccg gtctttcttt
     1081 ttttcacttt cgccagttct tccgcagagt aatgcgacgc gacatccagc cgtcgaagcc
     1141 ccattttgat gtactcgctg ttgatctcaa taccaatgaa ttttcgcccg ctggagacgg
     1201 ctacggcacc ggtagtaaag ctaccggcaa acgggtcgag aacgatatcg cctgggttgg
     1261 aagaggcgag aataatgcgt ttcagtaagg cttccggttt ttgcgtcggg tggttctcat
     1321 attcatccat caaataacgc acgcgcggaa aatcccagac gttacccggt actttttgat
     1381 gattgtatgg ctgtggagga tttttgcgat aatcgatcaa cgcgcgctgc gatccggttt
     1441 tggcttctac cagaatagca tcaccgttga atgtgtagtt ctttgcgtct ttcaccatca
     1501 tcaggatggg ttcgtacatg gagccgtagt gttttttcgc ctgtactcca gaactgtcat
     1561 atgaccagac gatgcgactt ttgatggtaa aaagcttgcg gcactggaga tcgataaagg
     1621 gcatgttttc cgtgctgttc atgatgtaca tgctgccctg ctttttcaga acgcggtggc
     1681 actctgcaat cacttcaaac agccagtcga taaacagatc ttctttccag gcttcgatca
     1741 gaccatcaaa atttttaccg atgttatatg gtgggtcggc aaagatcaga tcgacacttt
     1801 cggcggggat ctttttaagt tcggcaagcg catcaccgtg aataatggtc ttagcttcat
     1861 taccaaaccg ggtcggttca catcctgttc tcatggtcac tccctttgtg acacctataa
     1921 aaaaggcgct tccccatgcc gagtagcgcc tttttaatca agcatttagc taacctgaat
     1981 tagttcatgc cgtatttttt caatttttta cgcagcgtac cacggttgat gcccatcatc
     2041 agcgcagcac gggtctggtt accacgggtg tattgcatca ccatgtccaa caggggctgt
     2101 tctacttcag ccagtaccag ctcatagagg tcattcacat cctgaccatt cagttgagca
     2161 aaatagttct tcagtgcctg tttaaccgag tcacgcaggg gtttttgggt tacctgatcc
     2221 tgagagttaa cggtagaaac ggtcagtacg tcagaattta cgcgttgttc gaacatagtt
     2281 ctgtcagctc tttatttctg tttacgcaaa attttcgaag tatgcctcca acgcctccag
     2341 ctgttcgctg gcatcctcaa tggcgttgaa tgtgcgccga aactggtcat ttggagcgtg
     2401 ttcctggaga taccaggaaa cgtgtttacg tgcaattcgg tacccttttg ccggaccata
     2461 aaagtcatgc agttcccgaa cgtgcgcgca aagcaagcgc ttaacctctg ccaaaggcag
     2521 cgggggcagc aactccccag tgtccagata atgctggatt tcccgaaaga tccagggtct
     2581 tccctgagct gcgcggccta tcatcagggc atccgcccct gtatagtcga gcacagctct
     2641 ggctttaagc gggtcagtaa tgtcgccatt cgcgataacc ggaatggaaa ctttctgctt
     2701 aactgcccga atactgtcgt actcagcttc tccattgaac aaacaggcgc gtgtacggcc
     2761 atgaatggtc agagcctgaa tgccacagtc ttcagccagt tgggcaatct cttcgcagtt
     2821 acggtgttcc ggtgcccagc cggtgcgaat cttcagggta acaggaacgt ccactgcatt
     2881 gacgacctcg gtaaggatcg atttaacgac atccgggtac tgcaagaggg ctgaacctgc
     2941 gagcttgcga ttcacttttt tagccgggca acccatattg atatcaataa tctgggcacc
     3001 gctttccacg ttaatacgtg ctgcatctgc catttctttc ggatcgctac cagcaatttg
     3061 cacggtgcga ataccgggtt catcaatgtg caccatccgt aaacgagatt tgtcgctttc
     3121 ccaaacctgt gggttagaag acatcatctc ggatactgtc aatccggctc ccatctcgta
     3181 gcacaacgtc cgaaaaggtc tgtctgtaat gccagccatg ggcgctgcga tcaggcgatt
     3241 tctgagctga tattgtccga tgcgcatgag ttaagaaatg accatactgt gactgcaagg
     3301 cggcgtatat tacgcatttt ttgcacgaga tgaaaggcca aactttgacc aatcctctga
     3361 gatggatcaa agaattgcat ttaaaatgag cgtggtgcga taattactca taaaaatcat
     3421 catattagaa aatagtgact aaaatttaca ctcaaagaaa tttgagtaag ttctcaattt
     3481 ttctttatga atgaaaattt tggcacgcaa atttgcgtaa ataatcggca aatttacgtg
     3541 ccttttgtga gcttgctcgc acttcgcccc gcgtcaccct acggcgatgc gaaggttaat
     3601 tcttacgacc ggtaatacgg caccactctt ctttttccac gaccgggtcc agtgcgaagc
     3661 tgtcggcata agcttcacaa acgctctctg cctggcttgc cagaataccg gaaaggccca
     3721 gcaaaccgcc tgaaaccggc aggacgctga ttaacggtgc cagttcacgt aatgggcctg
     3781 caaggatgtt agcgaccacc acgtcggctt tcatttcttc tggctgatct ttcggtaagt
     3841 agagttccag acggtcagaa acgccattac gttcggcgtt gtcgcggctg gcctgaatcg
     3901 cctgcggatc gatatcaata ccaatggctt ttgctgcacc cagtttcagc gccgcgatcg
     3961 ccagaatgcc ggaaccacag ccaaagtcga tgactgtttt accggttaaa tcgaggctgt
     4021 cgagccattg caggcacaga gaggtggttg gatgggtacc cgtaccaaac gccagccctg
     4081 gatctaacat cacgttgacg gcgttttcgt ccggcacatc acgccagcta gggcagatcc
     4141 acagtcgttc accaaagcgc atcgggtgga aattatccat ccattcgcgc tcccagtctt
     4201 tatcttctag ttgttcgatt ttatgcgcga agcctgcgcc gagcagcgga tggttttcca
     4261 gaatcgccac cacgtcgttc atatcggttt cagcgtcaaa cagaccaatc acatcggtgt
     4321 cgccccacag gcgcgtttcg cccggcagcg gctcaaatac tggcgtatcg tgggtatcct
     4381 gaaaagtgat agaaacggca cccgcttcca tcagcgcatc gctaagatct tccgcgttcg
     4441 cgccggtggt gttcagtttc agttggatcc aaggcatggc aaaactcttt atttatcagt
     4501 agtcaaaacg gtagcttgcg ggacggatgt accgaaacgg tttccgacca ggaaagccag
     4561 caaacttagt agtaacgagg gcacgatagg gtggaagccc aggtactgaa tattcagcgt
     4621 cgcgagtacg gcatacagca cgccgccaac gatcatcgca cttagcgcgc ctttggcgtt
     4681 ggcgcgttcc cagtaaagac ccagcaccag cggccacagg aaaacggctt ccagcccacc
     4741 gaaggccagc aaattcagcc agatgatcat ttctggcggc ttccaggcgg caagcagcag
     4801 caacgcgccg agaactaacg taattaccgc cgacatccgc ttcagacgcg tctcgttttg
     4861 catttgatcc ggacggatat tcagatagag atctttaatg atcgtagcgg aactttgcag
     4921 caactgggca ttgatcgtcg acatgatcgc agccataggt gcagccagga agattccggc
     4981 agcaaacggt ggcagcactt ttaccattaa cgtcgggatc accagatccg gtacggtgag
     5041 atcggggatc actgcacgac ctaacgctcc ggccaggtgc ataccgaaca tcaggattgc
     5101 gactacaatc gtaccgatga tgatcccccg gtgtactgct ttgctgtctt tataagagat
     5161 acagcgcacc gcagtatgcg gcaggccaat cacgccaaag cacaccagta cccagaacga
     5221 cgtcataaag gcaggcgaca gaatatcgtc agcgccttgt ggcgtaacca gttgcggatc
     5281 gatggtttgc aaggtctgta ctgcgttgct taagccgcca gcggcatgta ccacgccaat
     5341 aagcagcaca acggtgccaa tcagcatcac aagcccttgc atggtgtcgt tcagcacgct
     5401 ggcgcgaaag ccaccaaagg cggtatataa cgcaatgctg ataccaaaaa tcagcagccc
     5461 ggtttcataa ggaatacccg ccgcggtttc cagcaggcgc gcaccgccga taaactgcac
     5521 ggtcatcgca ccaacgaacg caaccagcaa actcaaactc gccagccaca ccagaagacg
     5581 actctggtag cgggcaaaca gcatatcgtt cagcgtcact gcattgtagc ggcgcgcaag
     5641 aatcgcaaac ttcttgccga gaataccgag tgaaagccag actgcaggaa gctgaatcat
     5701 cgccagcaat acccagccca gcccgtattt ataagcagct cctggcccgc cgataaacga
     5761 actggcactg atataggtcg cggtgagcgt catcgccagc acaataccgc ccatagagcg
     5821 gctgccgagg aaatactcat taaggaaggt gccggtgctc cgtttacgca tcgcataaac
     5881 cgagataccg aacaccacca ccagataggc gaccagcggt agaattactt caagctgcat
     5941 cgtcatcctc cagtgggata tcgcgataga taaatttcac catcgcccag cacagtccaa
     6001 taaacagcag cggcgtcagg atgcaggcca tctcaaacca gcgcggaaag ccggtaaaac
     6061 cgggggcaac gccaggtaag taagcggcta ctaaccatac cgccagatac aaaagggtca
     6121 gccccagcgc ccagcgcgcc tctttatggg cctgaacaaa acgagtgtcc attttttgtc
     6181 cctgatgggt gaagaaagcg aggattgtac cttatggggg ttgtcgatcc ccagtaataa
     6241 aaaaggccgg aaaatccggc cttttgacgc ttttgcagtc ttatttttcc tgaagaccga
     6301 gttttttctc cagatagtgg atgttagtgc caccatgctg gaagttctcg tcattcatga
     6361 tgcggatctg cagatcaacg ttggttttga taccgtcgat gatcagctcc tgcagcgcat
     6421 tcttcatgcg ggcaatcgcc acgtcacggt tttcaccgta gcaaatcagc ttaccgatca
     6481 ttgagtcata gtacggcggt acggtgtagc ccgcgtagat atgagactcc caacgcactc
     6541 caaaaccgcc aggtgcgtgg aaacgggtga ttttgcctgg gcttggcagg aaggtgttcg
     6601 gatcttcggc gttgatacga cattccaccg catggccgcg aacgtgaact tcttcttgct
     6661 tgatcgacag cggttgaccg gcagcgatac gcagctgttc tttgatcagg tcaacgccgg
     6721 tgatcatttc agtaaccggg tgttctacct gaatacgggt gttcatttcg atgaaataga
     6781 actcgccgtt ttcgaacagg aactcgaaag tacctgcacc gcgatagccg atatcaacac
     6841 acgctttagc gcaacgttcg ccgatgtagc gacgcagttc cggggtaatg cccggtgctg
     6901 gtgcttcttc gaccactttc tggtggcggc gctgcatgga gcagtcacgt tccgccagat
     6961 agatagcgtt gccctgaccg tcagccagta cctgaatctc gacgtggcga ggattttcca
     7021 ggtatttctc catgtaaacc atatcgttgc tgaaagcagc tttcgcttcc gcacgggtca
     7081 tggagatgga ttgtgccagt tcagcgtcgc cgcgcactac gcgcataccg cgaccaccgc
     7141 cgccgccgga ggctttgata atcaccggat aaccaatgcg tttagcaatg gcacggtttt
     7201 tatccatatc gtcgcccagc gggccgtcag aacccggtac gcaagggacg cccgcttttt
     7261 tcatcgccgc gattgcggat actttgtcgc ccatcaggcg aatggtttct gctttcgggc
     7321 caatgaagat aaagccggag cgttcaacct gctcggcaaa gttggcgttc tcggagagga
     7381 agccgtaacc cggatggatt gctactgcgc cggtgatttc agcggcgctt atgattgccg
     7441 ggatgttcag ataacttttt actgacggag cagggccaat acagaccgtt tcatctgcca
     7501 gtaatacgtg ttttagatcg cgatccgcgc tggagtgcac agcgacagtc ttgatgccca
     7561 gttctttaca ggcacgaaga atacgcaatg caatctcgcc gcggttggca ataacaattt
     7621 tatccagcat gttcgcctcg ttactcgatg acgaccagcg gctcgtcaaa ttctaccggt
     7681 tgtccacttt cgaccagaat tgctttcacg gtaccggatt tgtccgcttc gatctggttc
     7741 atcattttca tggcttcaac gatgcacagg gtatcgccca cgttgacttt ctgacccact
     7801 tcgatgaacg cttttgcgtc cgggcttggg gtgcggtaga aagtaccaac catcggggag
     7861 cgtacgatgt gaccactgat ttccgctgct gctggcgctt ccatggaagg aacggtcgcc
     7921 ggagcggctg cgttagattg agctggctgc tgcatcattg gtgcagcgta agcttgttgc
     7981 atcacaggga aacttgcggc aggagctgca cggctgatgc gtactgactc ttcgccttcg
     8041 gagatttcca gttcggagat gcctgattct tcaaccagct cgatcagttt tttaatctta
     8101 cgaatatcca tgagtgggtt ccgtactctt tgtttagtgt gattgtgaca ggcgtttcac
     8161 cgccgtctgt aaagcgtatg ccccgacagc tgtatgcata gcgataaatt ccagcaggcc
     8221 gggacgccgg tctattttgc cccagagtgt caatgttaga cttgacggac attgtgcagc
     8281 gatctgctgc tatcctccgg caaaaaacaa aatatacctt cgttgcgcca atgtcacctt
     8341 ttccgcagcg caaaaactgc gtcagggagg acgaggtcgc acattataac gatttcgtag
     8401 caattggcag ctaaatactg gtcttatcag ggaagataat caacagctaa catgtaaata
     8461 accttcaaca ccgtgtaatt tgcaacaagc cgcacaattc acgaaattag cgccatcatc
     8521 gacggaactt cttataacgt aaggtgaaaa gctgtaaagc cagccctgcg aagatgaggg
     8581 gctgcaatga gataatcttc acagaccaca aataatgtag ggatgccagg atcgcgacaa
     8641 gatagacgac attacgcagc ggttgctggg gattacccaa ttttcactgc atcgtctgga
     8701 tagatgtgaa tgctaaagca cgcaaaatca accgactgac aattctcagc attaaatagg
     8761 ttcgagtggt taatttgtag tcaaataaaa tcagattatt caccccatgg gaccagaaat
     8821 cagtaacagg ttaagcgtag tgtcgcccag gcaaagcacc ataaacctaa caggcgacgg
     8881 aggcatatca ataatggctg tttagaggag tatcaatgcg catcaatttg tggtcagtcg
     8941 aaatcatgac tttccagttt ggatgatgtt gtacaacttc ctgaccaagg tgttcgacga
     9001 aagctgtccg gctaaccgct tttcccgtta accgattgaa taatgatgtc aaaatgagtt
     9061 ccctctctta ttattccttg ctaaatggtt agttaacctt caccagcgtg cgaccctgga
     9121 tctggttatt aatgatggcc tcggcaaagt tcggtgcctc tgacagagat atctcttttg
     9181 ccgcctgggt atagaatgat tccggtaaat cggcgaccag tcgctgccag gcttgtgcgc
     9241 ggcgttctgg tggcgtcatt actgaatcca ccccttgcaa acggacatta cgcagaataa
     9301 atggcatgac cgtggttggc agagtaaaac cacccgccag accacaggcc gccacgcagc
     9361 cgccgtaatt catttgcgcc agcacttttg ccagcacttt gtcgccaaca gtgtcaattg
     9421 ccccagccca gacttgtttt tccagaggac gggattcggc aaactcatca cgagggagaa
     9481 tacgactggc acctaaactt ttcagatatt catgggtact ttcgcgaccg gaaacggcaa
     9541 cgacctgata acccaactta tgcagcagcg ccacggcggt actgccgacg ccaccactgg
     9601 cacccgtcac gacaatctcc ccgtcctgcg ggcgaacacc ggcatcttcc agcgccatca
     9661 cacacagcat ggcggtaaaa ccggcagtac cgataatcat tgctttacgc gcgtccagcc
     9721 cttgcggcat ggcaaccagc cagtcacctt tcactcgcgc ctgctccgcc agcccacccc
     9781 agtggttttc accaacgccc cagccagtga gtaacacctc ctgaccggca tgaaaacgcg
     9841 gatcttcgct ggtgcgtaca gttccggcaa aatcgatccc aggaatcatc ggaaaattac
     9901 ggatgatttt tcccttaccg gtaatcgcca gcgcatcttt atagttcagg ctcgaccagt
     9961 gaacatcaac cgtgacatcg ccctccggca ggcgactttc gtcaagtgtc tgtaccgatg
    10021 cgagagtttt gccgtcctgc tgttctaaaa gtaacgcctg cataagtggt cctcatgtgc
    10081 atgatggatt ggaaaataat ttctgaagac tatactcgct aatggaaaag caaagccgat
    10141 gaagcgcaat aaattgcgag ataaatctga tttgctagta tgcccgcttc ctcactatcg
    10201 gagttaacac aaggatgaga ttaacgacga aattttcggc ctttgttacg ctgctcaccg
    10261 ggttaacaat ttttgtgact ttgctgggct gttcgctaag tttctacaac gcaattcagt
    10321 ataagtttag tcatcgcgtt caggcggtgg cgacggcgat tgatacccac cttgtgtcga
    10381 atgacttcag cacattaagg ccacaaatta ccgaattaat gatgtcggca gatatcgttc
    10441 gtgtagacct gctccatggt gataaacagg tttataccct ggccagaaat ggtagttatc
    10501 gtccggttgg ctccagcgat ctgtttcgcg aactgagtgt tccgttgata aagcatccgg
    10561 ggatgtcgct gcgtctggtt tatcaggatc cgatgggcaa ctatttccat tctttaatga
    10621 ctaccgcgcc gctcacgggg gcgattggct ttatcattgt tatgctcttt ctggcggtac
    10681 gctggttaca acggcaactt gccgggcaag aattgctgga aacccgggct actcgtatct
    10741 taaacggtga gcgtggctct aatgtgttgg gaaccatcta tgaatggccg cccagaacca
    10801 gcagtgcgct agatacgctg cttcgtgaaa ttcagaacgc acgcgaacaa cacagccgtc
    10861 ttgatacgct gatccgctct tatgccgccc aggacgtgaa aaccggcctc aataaccgac
    10921 tctttttcga taatcagtta gcaacgttac tggaagatca ggagaaagta ggtacccacg
    10981 ggatcgtgat gatgattcgt ctgccggatt tcaatatgtt gagcgatact tgggggcaca
    11041 gccaggttga agaacagttc ttcactctga cgaatctgct gtcgacattt atgatgcgct
    11101 accctggcgc actgctggcg cgttaccacc gcagtgattt tgctgcgctg ttaccgcacc
    11161 ggacgttaaa agaggcagag agcatcgccg gtcagttaat caaagccgtc gataccttgc
    11221 cgaacaataa aatgctcgat cgcgacgata tgatccacat tggtatctgc gcctggcgta
    11281 gtggtcagga taccgagcag gtaatggaac atgcagagtc tgccacgcgt aatgcgggat
    11341 tgcagggcgg caatagctgg gctatttacg atgactcgtt gcctgaaaaa ggacgcggta
    11401 atgttcgctg gcgtacgctt atcgagcaaa tgctcagtcg cggcggcccg cgcctttatc
    11461 aaaaaccggc ggttactcgc gaaggtcagg ttcatcatcg cgaactcatg tgccgcatct
    11521 tcgatggtaa tgaagaggtt agctcggcgg agtatatgcc gatggtcttg cagtttggct
    11581 tatcggaaga gtatgaccgt ctgcaaatca gccgtcttat tccactattg cgttactggc
    11641 cagaggaaaa tctggcgatt caggttaccg ttgagtcgct gattcgcccg cgttttcagc
    11701 gttggctgcg cgatacgtta atgcaatgtg aaaaatcaca acgaaaacgc ataattattg
    11761 aacttgcaga ggccgatgta ggtcaacata tcagtcgttt acaacctgtt attcgtttag
    11821 tgaatgcttt aggggtacgg gtagccgtca accaggctgg tttgacgctg gtaagcacca
    11881 gttggatcaa agaacttaat gttgagttac tcaagctcca tccggggctg gtcagaaaca
    11941 ttgagaagcg aacggagaac cagctgctgg ttcaaagcct ggtggaagcc tgctccggga
    12001 ccagcaccca agtttacgcc accggtgtgc gttcgcgaag cgagtggcag accctgattc
    12061 agcgcggtgt tacaggcggg caaggggatt ttttcgcgtc ctcacagcca cttgatacta
    12121 acgtgaaaaa atattcacaa agatactcgg tttaacctgc cgtttaatcc gttttcacgt
    12181 agaataacgc gcgctgcgtc tcatgggagt gtgcttgtct gctcgccaga ttgttgcagc
    12241 acatatgcag atgaatgacc ttacgcggtt gcaaacaggc gaggaatgct gctgatgcat
    12301 taagcctttc tggactcagg cagagatttg taacaaagga aacgaactgc actaattttc
    12361 accgtagcag atgatttttg cgccttgtcg ctgctgcgtg tggttggtaa agtaagcgga
    12421 ttttcttttc cgccccagct ttcaggatta tcccttagta tgttgaaaaa atttcgtggc
    12481 atgttttcca atgacttgtc cattgacctg ggtactgcga ataccctcat ttatgtaaaa
    12541 ggacaaggca tcgtattgaa tgagccttcc gtggtggcca ttcgtcagga tcgtgccggt
    12601 tcaccgaaaa gcgtagctgc agtaggtcat gacgcgaagc agatgctggg ccgtacgccg
    12661 ggcaatattg ctgccattcg cccaatgaaa gacggcgtta tcgccgactt cttcgtgact
    12721 gaaaaaatgc tccagcactt catcaaacaa gtgcacagca acagctttat gcgtccaagc
    12781 ccgcgcgttc tggtttgtgt accggttggc gcgacccagg ttgaacgccg cgcaattcgt
    12841 gaatccgcgc agggcgctgg tgcccgtgaa gtcttcctga ttgaagaacc gatggctgcc
    12901 gcaattggtg ctggcctgcc ggtttctgaa gcgaccggtt ctatggtggt tgatatcggt
    12961 ggtggtacca ctgaagttgc tgttatctcc ttgaacggcg tggtttactc ctcttccgtg
    13021 cgcattggtg gtgatcgttt cgacgaagct atcatcaact atgtgcgtcg taattacggt
    13081 tctctgatcg gtgaagccac cgcagaacgt atcaagcacg aaatcggttc ggcttacccg
    13141 ggcgatgaag tccgtgaaat cgaagttcgt ggccgtaacc tggcagaagg tgttccacgc
    13201 ggttttaccc tgaactccaa cgaaatcctc gaagcactgc aggaaccgct gaccggtatt
    13261 gtgagcgcgg taatggttgc actggaacag tgcccgccgg aactggcttc cgacatctcc
    13321 gagcgcggca tggtgctcac cggtggtggc gcactgctgc gtaaccttga ccgtttgtta
    13381 atggaagaaa ccggcattcc agtcgttgtt gctgaagacc cgctgacctg tgtggcgcgc
    13441 ggtggcggca aagcgctgga aatgatcgac atgcacggcg gcgacctgtt cagcgaagag
    13501 taatcggatg caggcagggg aaatgtctgt ttaccctgcc tggtctgata cgagaatacg
    13561 cataacttat gaagccaatt tttagccgtg gcccgtcgct acagattcgc cttattctgg
    13621 cggtgctggt ggcgctcggc attattattg ccgacagccg cctggggacg ttcagtcaaa
    13681 tccgtactta tatggatacc gccgtcagtc ctttctactt tgtttccaat gctcctcgtg
    13741 aattgctgga tggcgtatcg cagacgctgg cctcgcgtga ccaactagaa cttgaaaacc
    13801 gggcgttacg tcaggaactg ttgctgaaaa acagtgaact gctgatgctt ggacaataca
    13861 aacaggagaa cgcgcgtctg cgcgagctgc tgggttcccc gctgcgtcag gatgagcaga
    13921 aaatggtgac tcaggttatc tccacggtta acgatcctta tagcgatcaa gttgttatcg
    13981 ataaaggtag cgttaatggc gtttatgaag gccagccggt catcagcgac aaaggtgttg
    14041 ttggtcaggt ggtggccgtc gctaaactga ccagtcgcgt gctgctgatt tgtgatgcga
    14101 cccacgcgct gccaatccag gtgctgcgca acgatatccg cgtaattgca gccggtaacg
    14161 gttgtacgga tgatttgcag cttgagcatc tgccagcgaa tacggatatt cgtgttggtg
    14221 atgtgctggt gacctccggt ctgggcggtc gtttcccgga aggctatccg gtcgcggttg
    14281 tctcttccgt aaaactcgat acccagcgcg cttatactgt gattcaggcg cgtccgactg
    14341 caggactgca acgtttgcgt tatctgctgc tgctgtgggg cgcggatcgt aacggcgcta
    14401 acccgatgac gccggaagag gtgcatcgtg ttgctaatga acgtctgatg cagatgatgc
    14461 cgcaggtatt gccttcgcca gacgcgatgg ggccaaagtt acctgaaccg gcaacgggga
    14521 tcgctcagcc gactccgcag caaccgacga caggaaatgc agctactgcg cctgctgcgc
    14581 ccacacagcc tgctgctaat cgctctccac aaagggctac gccgccgcaa agtggtgctc
    14641 aaccgcctgc gcgtgcgccg ggagggcaat agtggcgagc tatcgtagcc agggacgttg
    14701 ggtaatctgg ctctctttcc tcattgcgct gttgctgcaa atcatgccct ggccggataa
    14761 cctgattgtt ttccggccaa actgggtgtt actcatcttg ttgtattgga tcctggcctt
    14821 gcctcatcgc gtaaatgtgg gcacaggttt tgtgatgggt gccatactgg atctgatcag
    14881 cggctcgacg cttggcgtac gcgtattggc gatgagcatc attgcttacc tggtggcgct
    14941 gaaataccag cttttccgca acctcgcatt atggcagcag gcgctggtcg tcatgttgct
    15001 ttcgctggtg gtggatatta ttgttttctg ggcagagttt ttagtgatta acgtctcttt
    15061 cagaccggaa gtgttctgga gtagtgtagt caatggggtg ctctggccgt ggattttctt
    15121 gctgatgcgt aaagtccgtc agcagtttgc agtgcaataa aggtttctat gacttctctg
    15181 tatttagctt ccggttctcc gcgtcgtcag gagttacttg cgcaacttgg cgtgaccttt
    15241 gaacgtattg ttacgggcat tgaggagcag cgtcagccgc aggagagcgc gcagcagtat
    15301 gttgtgcgtc tggcgcgcga gaaagcacag gcgggtgtcg cgcaaacggc gcaggatctc
    15361 ccggtgctgg gtgcggatac tatcgttatc ctgaatggag aagtgctgga gaaaccgcgc
    15421 gacgcagagc atgcggcgca gatgttgcgc aaattatcgg gtcagaccca tcaggtgatg
    15481 acggcagtgg cgttggccga cagccagcac attctcgatt gcctggtggt caccgatgtg
    15541 actttcagaa cgttaacaga cgaagacatc gcgggctatg tcgccagcgg tgaaccgtta
    15601 gataaagcag gtgcatacgg tattcagggg ctgggtggct gttttgtcag gaagataaat
    15661 ggcagctatc acgccgtagt cggcttaccg ctggttgaaa cgtatgaatt attaagtaat
    15721 tttaacgcac tgcgtgagaa aagggataaa catgacggct gaattgttag taaacgtaac
    15781 gccttcggaa acgcgagtgg cgtatattga tggcggtatt ctgcaggaaa ttcatattga
    15841 acgtgaggcg cgacgcggaa tagtaggcaa tatctacaag ggtcgtgtaa gtcgtgtact
    15901 tccgggtatg caggcggctt ttgtagatat tgggctggat aaagccgcgt ttcttcatgc
    15961 atctgacatc atgccgcaca ccgaatgtgt ggcgggtgaa gaacaaaagc aattcacggt
    16021 gcgcgacatc tcggaactgg ttcgtcaggg gcaagatctg atggtgcagg tggtgaaaga
    16081 tccgcttggc actaaaggtg cgcgcctgac caccgatatc acgctgcctt ctcgctatct
    16141 ggtgtttatg ccaggggctt ctcacgttgg ggtttcccaa cgtattgaaa gcgaatcaga
    16201 acgtgaacgc ctgaaaaaag tggtcgcaga gtattgcgac gagcagggcg ggtttatcat
    16261 ccgtaccgca gcggaagggg ttggcgaggc tgaactggcc tccgatgccg cttatctgaa
    16321 acgcgtctgg accaaagtta tggagcgtaa aaaacgcccg cagacccgtt atcagctgta
    16381 cggcgaactg gcgctggcgc agcgtgttct gcgtgatttc gccgacgccg aactggaccg
    16441 cattcgcgtt gactcacgcc tgacttacga agcattgctg gagtttacct cggagtacat
    16501 tcccgagatg accagcaagc tggagcatta caccggacgc cagccgattt tcgatctctt
    16561 tgatgtcgaa aacgaaatcc agcgagcgct ggaacgcaaa gtagaactga aatccggtgg
    16621 ttatctgatt atcgaccaga ccgaagcgat gaccaccgtg gacatcaata ccggagcgtt
    16681 tgtcggtcat cgcaatctgg acgacaccat tttcaatacc aatattgaag cgacgcaggc
    16741 tatcgctcgc cagttacggt tgcgtaatct gggtgggatt atcattattg atttcatcga
    16801 tatgaataat gaagatcacc gccgccgcgt gctgcactcg ctggagcagg cgttgagcaa
    16861 agaccgggtg aaaaccagcg ttaatggttt ttcggcgctg gggctggtgg agatgacgcg
    16921 taaacgcacc cgcgaaagca ttgagcacgt actgtgtaac gaatgcccaa cctgccacgg
    16981 tcgcggaacg gtgaaaaccg tggaaacggt atgctatgaa atcatgcgcg agattgttcg
    17041 tgtccaccat gcttacgact ccgaccgttt cctggtctat gcatctccgg cagtagctga
    17101 agccttgaaa ggcgaagagt cacactcgct ggcggaagtg gaaattttcg ttggcaaaca
    17161 ggttaaagta caaattgaac cgctctataa ccaggagcag tttgacgtcg taatgatgta
    17221 aacagatgct gggccgccat ccggcaaagg gtttttgagt cacattttta gcagacaagg
    17281 agtgacgggt gaggcgattg ccggggattt tactgcttac tggagccgcg ctcgttgtga
    17341 tcgctgctct gctggttagc ggcctgcgta ttgctttacc gcatcttgac gcctggcgtc
    17401 cggaaatcct caacaaaata gaatccgcga ctggcatgcc ggtagaagcc agtcagctct
    17461 cagccagctg gcagaatttt ggcccgacgc ttgaagcaca cgacatccgt gcagaactaa
    17521 aagatggcgg cgaattttcg gttaaacgcg ttactctggc gctggatgtc tggcagagcc
    17581 tgttacatat gcgctggcag tttcgcgacc tcactttctg gcagctgcgc tttcgcacca
    17641 acactcctat caccagcggt ggtagcgatg acagtctgga agccagtcac atcagcgatc
    17701 tgtttcttcg tcaatttgac catttcgatc tccgcgacag tgaagtcagt tttctgacgc
    17761 catccggtca gcgcgccgag ctggcgatcc cacaactcac ctggctgaac gatccacgtc
    17821 gacaccgtgc ggaaggcctg gtaagcctct ccagccttac cggacagcac ggcgtgatgc
    17881 aggtgcgcat ggatttgcgc gatgatgagg ggttgttaag caatggtcgc gtctggctac
    17941 aggcggatga catcgacctg aagccgtggc tcggtaaatg gatgcaggac aatattgctc
    18001 tggaaacggc acagttctcc cttgaaggct ggatgacgat cgacaaaggc gatgtaaccg
    18061 gcggtgacgt ctggctgaaa cagggcggtg ccagctggtt gggcgagaag caaacgcata
    18121 cgctgtcggt ggataatctg accgcgcata ttacgcgtga aaatccgggc tggcagttct
    18181 ctattcccga tacacggatc acgatggacg gcaaaccctg gccgagcgga gcattgacgc
    18241 tggcctggat accggaacag gacgttggcg gcaaagacaa taaacgcagt gacgaactcc
    18301 ggattcgcgc cagtaatctg gagctggcag gcctggaggg catacgcccg ctggccgcga
    18361 aactttcacc tgcactgggt gatgtttggc gctccacaca accgagcggc aagattaaca
    18421 ctctggcgct ggatatcccg cttcaggcgg cagacaagac ccgttttcag gcatcgtgga
    18481 gcgatctggc ctggaagcaa tggaaattat taccgggtgc ggaacacttc tccgggacgc
    18541 tttccggcag cgttgaaaat ggtttgctta ccgcgtcgat gaagcaggca aagatgcctt
    18601 acgaaacggt attccgtgcg ccactggaaa tcgccgacgg ccaggcaact ataagctggc
    18661 tgaacaatga caaaggtttc cagctggatg ggcgtaatat tgacgttaaa gccaaagccg
    18721 tccatgcgcg cggcggtttt cgttacctgc aacctgctaa cgatgaaccc tggctgggta
    18781 ttctggctgg catcagtacc gatgatggtt cacaagcctg gcgctatttc ccggaaaact
    18841 tgatgggtaa agacctggtt gattacttaa gtggcgcgat tcagggcggt gaagcggata
    18901 acgcgacgct ggtttatggt ggcaatccgc aactcttccc ctataaacac aacgaaggtc
    18961 agtttgaagt gctggtgccg ctgcgcaacg cgaagtttgc cttccagccg gactggcctg
    19021 cattaactaa ccttgatatt gaactggact ttattaacga cggtttatgg atgaaaaccg
    19081 atggcgttaa tctgggcggc gtgcgcgcga gtaatctcac cgcagtgatc cctgactact
    19141 cgaaagaaaa actgctgatt gacgctgaca ttaaaggtcc gggtaaagcc gttggccctt
    19201 actttgatga gacaccgctg aaagattctc tgggtgcgac cctgcaagaa ctccagctcg
    19261 acggcgatgt gaatgctcgc ttacatcttg atatcccgct gaacggcgaa ctggtaaccg
    19321 cgaaaggtga agtgacgctg cgtaataaca gtctgtttat caaaccactc gacagcaccc
    19381 tgaaaaattt gagcggtaaa ttcagcttta tcaatggcga tctgcaaagt gaaccactga
    19441 cagcaagctg gtttaatcag ccgttgaacg tggatttttc caccaaagaa ggggcaaaag
    19501 cctaccaggt tgcggtgaat ctcaacggta actggcaacc ggcgaaaacc agcgttctgc
    19561 ctgaagcggt gaacgaagca ttgagtggca gcgtggcgtg ggatggtaaa gtgggcattg
    19621 atctgcctta tcatgctggc gcgacctata acgtagagat gaacggcgat ctgaataatg
    19681 tgagtagtca cttaccttca ccgttagcca aacctgcggg tgaaccacta gcggtaaacg
    19741 ttaaggttga tggcaatctc aacagctttg aattaaccgg acaggctggt gcggataacc
    19801 atttcaatag ccgctggttg cttggtcaaa agctgacgct cgatcgtgct atttgggcgg
    19861 cagacagtaa aacgctcccg ccgttgccgg aacaaagtgg cgttgaactc aatatgccgc
    19921 cgatgaatgg tgccgagtgg ctggccctgt tccagaaagg cgctgcggag agtgtcggtg
    19981 gtgcagcgag tttcccacaa cacataacgt tacgtacgcc tatgttgtca ctgggaaatc
    20041 agcaatggaa taacctgagt attgtttcgc aaccgacggc aaatggcacc ctggttgagg
    20101 cgcaagggcg tgaaatcaac gccacgctag cgatgcgtaa taacgcgccg tggctggcga
    20161 atatcaaata tctttattac aacccgagcg tggcgaaaac tcgtggtgat tcaacgccgt
    20221 catcaccttt cccgacaacg gagcgcatta acttccgtgg ctggccggac gcacaaatac
    20281 gatgcacaga gtgctggttc tgggggcaaa aattcggtcg cattgacagt gatatcacca
    20341 tttctggcga tacgttaacg ctgaccaatg gactgattga tactggtttc tcgcggctta
    20401 ctgccgatgg tgaatgggtt aataatccgg agaatgaacg tacctcgctg aaaggaaaac
    20461 tgcgcgggca gaaaattgat gccgccgcag aattttttgg tgtcacgacg cccatacgcc
    20521 agtcgtcatt taatgtggat tacgatttac actggcgcaa agcaccctgg caaccagatg
    20581 aggcgacgtt gaatggcatc attcatactc aactgggtaa aggcgaaatt accgaaatca
    20641 ataccggaca tgccgggcaa ttgctgcgct tattgagcgt tgatgccctg atgcgtaagc
    20701 tgcgttttga tttcagagac acttttggcg aagggttcta ttttgactcc attcgcagca
    20761 ccgcgtggat taaagacggg gttatgcaca ccgacgacac gctggtggat ggcctggagg
    20821 cggatatcgc catgaaaggg tcggtaaatc tggtacgtcg cgacctgaat atggaagcgg
    20881 ttgtcgcacc agagatttct gcgacggtgg gcgtggctgc ggcttttgcg gttaacccca
    20941 ttgttggcgc ggcagtattt gcagccagta aagtgctggg gccgctgtgg agcaaagtct
    21001 ccattttgcg ctatcacatt tcgggtccgc tggacgatcc gcaaatcaac gaagtgttgc
    21061 gccaaccgcg taaagaaaaa gcgcaatgat ttgacgaggg cgcgtaattg ccccaatctc
    21121 atatgataat cgttgccaaa ggccaacgag ccggaacata actgtaggcc ggatagggcg
    21181 ttcacgccgc atccggcagc cgtaaaaaat cctctactgc agtaactaac gagtagcaaa
    21241 aacgatgagt ctgaacctgg taagtgaaca attgctagcg gcgaacggcc tgaaacatca
    21301 ggacttgttc gcgatcctcg gtcaactggc cgaacgtcgc cttgattatg gcgatctcta
    21361 ttttcagtcg agctatcacg aatcctgggt tttagaagac cgcattatta aagatggttc
    21421 ttacaacatc gatcagggcg ttggtgtgcg tgcaatcagt ggtgaaaaaa ccggatttgc
    21481 ttacgctgac caaatcagcc tgctggcgct ggaacagagt gcgcaagcgg cgcgcaccat
    21541 cgtccgtgat agtggtgatg gcaaagtaca gacgctgggc gcggtagagc atagcccgtt
    21601 gtatacctcg gtagatccgc tgcaaagcat gagccgtgaa gagaagctgg atatcctgcg
    21661 tcgcgtcgat aaggttgccc gcgaagcgga caagcgcgta caagaagtga ctgccagcct
    21721 cagcggcgtt tatgaattaa ttctggttgc ggccaccgac ggcacgctgg cggcggatgt
    21781 tcgtccgctg gtgcgtcttt ccgtgagcgt tctggtcgaa gaagatggca aacgcgaacg
    21841 cggtgccagt ggcggcggcg gtcgttttgg ttatgagttc ttccttgccg atcttgacgg
    21901 cgaagtccgt gcggatgcat gggcaaaaga agcagtgcgt atggcgctgg tcaatctttc
    21961 tgccgttgct gcaccagcgg gcaccatgcc ggtagtactt ggcgcaggtt ggccgggcgt
    22021 gctgttgcat gaagcggtag gtcacggtct ggaaggcgac ttcaaccgcc gcggcacttc
    22081 agtatttagt gggcaggtcg gggagctggt ggcttcagaa ctgtgtaccg tggttgatga
    22141 cggcacgatg gtcgatcgcc gtggttcggt ggcgattgat gacgaaggta cgccaggcca
    22201 gtacaacgtg ctgattgaga acggcattct gaaaggctac atgcaggata aactcaacgc
    22261 gcgtttgatg gggatgacgc cgactggcaa cggtcgccgt gaatcctacg cccatctgcc
    22321 catgccgcgt atgaccaaca cctatatgct gccgggtaaa tcgaccccgc aggaaattat
    22381 tgaatccgtt gagtacggta tctatgcacc gaactttggt ggcggtcagg tggatattac
    22441 atccggcaaa ttcgttttct ccacttcaga agcgtatctg attgaaaacg gtaaagtaac
    22501 gaagccggtg aaaggcgcga cgttgattgg ttccggtatc gaaactatgc agcagatttc
    22561 gatggttggc aacgacctga aactggataa cggcgtgggt gtctgcggta aagaagggca
    22621 aagtttgccg gttggcgtgg gccagccaac gctgaaagtt gataacctga ctgttggcgg
    22681 tactgcgtaa taatttcatt attttcagaa ggataattaa tctttctacg tgcacgaacg
    22741 gtcccctcgc tcctctgggg ttagggtgag gggaacccgt tggcacaggt ttgtagaacg
    22801 taacagtaca atatgaatta cttctctttc ccgcgcccgt gcatctcctg aaacaattta
    22861 ccgacctcaa caaaataatc cgtcagcgag ttgatcacga cctgtacctt cagcggcagc
    22921 ttatcttttt cggtatataa cgcataaacc gggcgtggat ctgactgata acgcggcagc
    22981 aggatctcca gctcaccgcg attgatctcg ttgatcaccc acatcagcgg cacgtaggcg
    23041 atcccggcac ccgccgttag ccagcgcacc agcgtcatcg gatcattagt cacaaatctc
    23101 ccttgcggga tcaggcgagt cgagatccct tccggtgcaa tcagttcaaa ttcattgtcg
    23161 ggccgcacgc tgtattcaag ccatgaatgg ctacttaaat cggcgggttt ttccggtatg
    23221 ccgtattgtg tgagatagct tttcgcggcg cacaccacca ttggcatcgc gccaagacgg
    23281 cgggaaaaca ggctggaatc ctgcaacgcg ccgacgcgga tcaccacatc cagaccgtcg
    23341 gcaatcaggt cgggggctgg aattccggta accagattga cgctcaaacc tgggtattct
    23401 ttcagcattt tggctgtcag cccggcgaga acattttgtg ccatagttga agaacagcca
    23461 atgcgtagcg tcccgatggg ggtgttattg aaggcataca gttgctcatg aacatcctgc
    23521 acttcatgaa gcatacgacg gcagccctgg tagtaaattc taccggcttc ggtcaggcca
    23581 atgctgcgtg tgctacggtt taacagcttt acctgcaact catcttccag ttttgacacc
    23641 gtctgactga tggacgaaac gctcatctgt agctgtctgg cggcggcggt aaaagagcca
    23701 aattcaacta ctttggcaaa caccgacatg cgttttagtc gttccattat tcactctgac
    23761 ttaaaagtga tttagatcac ataatataga taacagcata acagttacgc taatatatta
    23821 aatatcaatc tacagcaatg ttgctctcgc ccggctttcc atgcccttct ctgcggcgac
    23881 agatgctgaa aataataacg cctgctctct ctatacaacc aaggtcaaca tgagtctgtt
    23941 tcccgttatc gtggtgtttg ggctgtcctt cccaccgata ttttttgaat tgcttttatc
    24001 actggcgatt ttctggctgg tgcgccgggt acttgtgcca acaggtatct acgactttgt
    24061 ctggcatccg gcgttgttca acaccgcgct ctattgctgc ttgttttatt tgatatcgcg
    24121 actgttcgtt tgaggttgaa gtgaaaacac taataagaaa attctcccgt acggccatca
    24181 cggtcgtatt agtcattctg gccttcatcg caatttttaa tgcctgggtc tattacaccg
    24241 aatccccctg gacgcgtgac gcgcgcttta gcgctgacgt cgttgcgatc gcgccggacg
    24301 tttctggact cattacccag gtgaatgttc atgataacca gctggtgaaa aaaggacaga
    24361 tactgttcac catcgaccag ccgcgctatc aaaaggcgct tgaggaagcg caagccgatg
    24421 ttgcttatta tcaggtactg gcacaggaga aacgccagga ggccggacgt cgtaaccgtc
    24481 tcggtgtgca ggcgatgtct cgcgaagaga tcgaccaggc taacaacgta ctacaaacgg
    24541 ttctgcatca gttagcgaaa gcgcaggcga cccgcgatct ggcaaaactg gatcttgaac
    24601 gcacggtgat ccgcgcgcca gcagatggct gggtgaccaa cctcaacgtc tataccggtg
    24661 agtttattac tcgaggatca acggcggttg cgctggtgaa acagaactcc ttctatgtac
    24721 tggcctatat ggaagaaact aagctggaag gggtgcgtcc ggggtatcgt gcagagatca
    24781 cgccgcttgg cagtaacaaa gtgctgaaag ggactgttga tagtgttgcc gctggggtca
    24841 ccaacgccag cagcacgcgt gacgacaaag ggatggcgac catcgactct aaccttgaat
    24901 gggtgcgtct tgcgcaacgt gttccggttc gtattcgtct cgacaaccag caagagaaca
    24961 tctggcctgc gggcaccact gctacagtgg tggtcactgg caaacaagat cgcgacgaaa
    25021 gccaggattc gttcttccgt aaaatggccc atcgcctgcg tgagtttggt taatcacgat
    25081 gggtattttc tccattgcta accaacatat tcgctttgcg gtaaaactgg cgaccgccat
    25141 tgtactggcg ctgtttgttg gctttcactt ccagctggaa acgccacgct gggcggtact
    25201 gacagcggcg attgttgccg ccggtccggc ctttgctgcg ggaggtgaac cgtattctgg
    25261 cgctattcgt tatcgtggct ttttgcgcat catcggcaca tttattggct gtattgccgg
    25321 actggtgatc atcattgcga tgatccgcgc accattattg atgattctgg tgtgctgtat
    25381 ctgggccggt ttttgtacct ggatatcctc gctggtacga atagaaaact cgtacgcgtg
    25441 ggggctggcc ggttataccg cgctgatcat tgtgatcacc attcagccgg aaccattgct
    25501 tacgccgcag tttgccgtcg aacgttgtag cgagatcgtt atcggtattg tgtgtgcgat
    25561 tatggcggat ttgctctttt ctccgcgatc gatcaaacaa gaagtggatc gagagctgga
    25621 aagtttgctg gtcgcgcaat atcaattaat gcaactctgt atcaagcatg gcgatggtga
    25681 agttgtcgat aaagcctggg gcgacctggt gcgacgcacc acggcgctac aaggcatgcg
    25741 cagcaacctg aatatggaat cttcccgctg ggcgcgggcc aatcgacgtt taaaagcgat
    25801 caatacgcta tcgctgacgc tgattaccca atcctgcgaa acttatctta ttcagaatac
    25861 gcgcccggaa ttgatcactg atactttccg cgaatttttt gacacgccgg tagaaaccgc
    25921 gcaggacgtc cacaagcagc tcaaacgcct gcggagagtt atcgcctgga ccggggaacg
    25981 ggaaacgcct gtcaccattt atagctgggt cgcggcggca acgcgttatc agcttctcaa
    26041 gcgcggcgtt atcagtaaca caaaaatcaa cgccaccgaa gaagagatcc tgcaaggcga
    26101 accggaagtc aaagtagagt cagccgaacg tcatcatgcg atggttaact tctggcgaac
    26161 cacactttcc tgcattctgg gaacgctttt ctggctgtgg acgggctgga cttccggcag
    26221 tggtgcaatg gtgatgattg cggtcgtgac gtcactggca atgcgtttgc cgaatccacg
    26281 catggtggcg atcgacttta tctacgggac gctggccgcg ctgccgttag ggctgctcta
    26341 ctttttggtg attatcccta atacccaaca gagcatgttg ctgctgtgta ttagcctggc
    26401 agtgctggga ttcttcctcg gaatagaagt acagaaacgg cgactgggct cgatgggggc
    26461 gctggccagc accataaata ttatcgtgct ggataacccg atgactttcc atttcagtca
    26521 gtttctcgac agcgcattag ggcaaatcgt cggctgtgtg ctcgcgttca ccgttatttt
    26581 gctggtgcgg gataaatcgc gcgacaggac tggacgtgta ctgcttaatc agtttgtttc
    26641 tgccgctgtt tccgcgatga ctaccaatgt ggcacgtcgt aaagagaacc acctcccggc
    26701 actttatcag cagctgtttt tgctgatgaa taagttccca ggggatttgc cgaaatttcg
    26761 cctggcgctg acgatgatta tcgcgcacca gcgcctgcgt gatgcgccga tcccggttaa
    26821 cgaggattta tcggcgtttc accgacaaat gcgccgcaca gcagaccatg tgatatctgc
    26881 ccgtagcgat gataaacgtc gtcggtactt tggtcagttg ctggaagaac tggaaatcta
    26941 ccaggaaaag ctacgcatct ggcaagcgcc accgcaggtg acggaaccgg ttcatcggct
    27001 ggcggggatg ctccataagt atcaacatgc gttgaccgat agttaagtca aaaccgacgc
    27061 caaaagcgtc ggttttttca tggctatact tagcgatgaa cggcagaact cgccgcgaaa
    27121 cgtgacggtg gcaacagatg aatatttata cctttgattt tgatgagatt gagagtcagg
    27181 aggattttta tcgcgacttc agccaagcct ttggtctggc gaaagataag gtacgcgatc
    27241 tcgactcact atgggatgtg ttaatgaacg atgtcctgcc gctaccactt gagattgaat
    27301 ttgttcatct gggagagaaa acgcgtcgcc gttttggcgc gttaatattg ctgtttgatg
    27361 aggcagagga agagctggaa gggcatttgc gttttaatgt tcgtcattag cgcaaaaaaa
    27421 agcccccgaa ccgggggcaa tatcgtcgga caagacgatg agggtttatt tgtacagttc
    27481 agccgtagcg tgccaggtgt caccgctacg agcttcagta atctggtagg ccgttgcgcc
    27541 tttctcttcc gcttttttgt tcagcatttc acgtatatcc attggcgaag acgccacacc
    27601 acttacggat acggtcccga ttgcttcacg gttttgtgct tgtgcagcat caatggagtc
    27661 ggcagcgaat gcaccgaaag agagaacaga aagtacgctt aatgcagcaa cagtggtttt
    27721 gattttcatg atttttacct cgacataatc tttttgctgg gtctttgttt cgtgaccctt
    27781 atcacaaaat caagtataca ctaattattg aactaattaa taccacgcta actgtttctg
    27841 ttgatactaa gtgttaaaaa tgtgaatgtt aataacaaaa aagaattaaa aatacggtta
    27901 tttatcgtta attctagtgt tttcaattag ataatgaatt ttgcataatg tgcttttttt
    27961 accttatgta ttcattgtgt gaatgacatg tcgcagtaaa acgcactatt cgttaaatta
    28021 atcgcagggg aaagcagggg gttgagaggg ataagtaaca ttttccccgc cgccagaagc
    28081 gacggggcag agattaaagc tcctggtcga acagctctaa aatcgcttcg tacaggtctt
    28141 tgacggtgaa accgttagca ggggtggtaa agatggtgtc atcgccagcg atggtgccca
    28201 gaataccttc tgctttgccc agtgagtcca gcaggcgagc aattaactgc gccgcgccag
    28261 ggctggtatg aatcacgaca actgcatcgt tgtagtcgat atccagcacc agatttttca
    28321 atggactgga ggtggttggt acacccagtt cagctggcag gcagtaaacc atttccattt
    28381 tggcattgcg tgtacgtaca gcaccaaact tggtcaacat ccgcgagact ttagactgat
    28441 taatattgtc aaagccttgc tcctgcaacg cggcgacgat ttcgccctgg gagctaaatt
    28501 tctcttcttt aagtaatgct ttaaatgctt taactagttc ttcttgctta gccgagcttc
    28561 gcataagtca cccgatatgg tggttgatac aacattattg tgcatacaga tgaattttta
    28621 tgcaaacagt cagccctgaa gaaggctgaa ataatgttat gaaagagcgg gattttatca
    28681 aatttcgtta ttgagaaaca tgcctgcgtc acggcatgca aattctgctt aaaagtaaat
    28741 taattgttat caaattgatg ttgttttggc tgaacggtag ggtatattgt caccacctgt
    28801 tggaatgttg cgctaatgca taagcgactg ttaattacgt aagttaggtt cctgattacg
    28861 gcaattaaat gcataaacgc taaacttgcg tgactacaca ttcttgagat gtggtcattg
    28921 taaacggcaa ttttgtggat taaggtcgcg gcagcggagc aacatatctt agtttatcaa
    28981 tataataagg agtttaggat gaaagtcgca gtcctcggcg ctgctggcgg tattggccag
    29041 gcgcttgcac tactgttaaa aacccaactg ccttcaggtt cagaactctc tctgtatgat
    29101 atcgctccag tgactcccgg tgtggctgtc gatctgagcc atatccctac tgctgtgaaa
    29161 atcaaaggtt tttctggtga agatgcgact ccggcgctgg aaggcgcaga tgtcgttctt
    29221 atctctgcag gtgtagcgcg taaaccgggt atggatcgtt ccgacctgtt taacgttaac
    29281 gccggcatcg tgaaaaacct ggtacagcaa gttgcgaaaa cctgcccgaa agcgtgcatt
    29341 ggtattatca ctaacccggt taacaccaca gttgcgattg ctgctgaagt gctgaaaaaa
    29401 gccggtgttt atgacaaaaa caaactgttc ggcgttacca cgctggatat cattcgttcc
    29461 aatacttttg ttgcggaact gaaaggcaaa cagccaggcg aagttgaagt gccggttatt
    29521 ggtggtcact ctggtgttac cattctgccg ctgctgtcac aggttcctgg cgttagtttt
    29581 accgagcagg aagtggctga tctgaccaaa cgtatccaga acgcgggtac tgaggtggtt
    29641 gaagcgaaag ccggtggcgg gtctgcaacc ctgtctatgg gccaggcagc tgcacgtttt
    29701 ggtctgtctc tggttcgtgc actgcagggc gaacaaggcg ttgtcgaatg tgcctacgtt
    29761 gaaggcgacg gtcagtacgc ccgtttcttc tctcaaccgc tgctgctggg taaaaacggc
    29821 gtggaagagc gtaaatctat cggtaccctg agcgcatttg aacagagcgc actggaaggt
    29881 atgctggata cgctgaagaa agatatcgcc ctgggcgaag agttcgttaa taagtaatta
    29941 attagcgaat aataaaaaac cggagcacag actccggttt tttgttttga gcactcgact
    30001 taattggttg ccggatattc ctgaatggtg acctgcagcg ttaactgctt atcatcacgc
    30061 atcactacaa ccgggatcac cgaaccaggg cgaatttctg ccacctgatc catcgtctcc
    30121 agagcagaga tggccggttt gttatccacc gaaataatca gatcgttgac ctgaataccc
    30181 gcattcgccg ccgggccgtc aggtgacact tcattaacca cgatcccttg cagttgatct
    30241 ataccaccgc cctgcgcgtg cagtggtgcg atctcgcgtc cgccgatacc aatgtagccg
    30301 cggatcacgc gaccatcgcg gatcagctta tccataattt tggttgctaa ctggaaagga
    30361 atcgcaaagc cgataccttc cggcgtttcg ccatcgttac tcttatcaaa cgacagcgtg
    30421 ttaatgccca tcagttctcc cagcgagttc accagcgcgc cgccagagtt accgtggtta
    30481 atggaagcat cggtttgcag gaagttttgc cgcccggtcg ggttcagacc gattcgaccc
    30541 gtagcactaa taatcccctg ggtaatggtc tgcccgaggt tgtacgggtt accgatcgcc
    30601 agtaccacgt cgccaatgtg cggtacgcga cgtgcattga tcggaatggt aggtaaaccg
    30661 ccagtggcat taattttaag taccgccaga tcggttagag agtcagatcc caccagcaat
    30721 gcttcaaata cacgtccatc ctgtaaggcg acgatgatct gatcggcgtc gttgatgacg
    30781 tgtttattgg tgatgatata accgcgttga tccatgatta caccggatcc cagggtgcgg
    30841 atctcaagct ggttgtgaga gttggtgttc aaaccacggt tgtaaacgtt aaccaccgct
    30901 ggcgcggcgc ggcgaaccgc cagattataa ctggcaggcg tctcatcggt actgtcaaat
    30961 tgcggagtgg aaagcgggtt aaggctgcgc agcgaaggca tggcaaccag cagaatagcg
    31021 ccgacaatta atccaatcgc aacggaacgt aagagcttca caaacatgat ggaggcgtca
    31081 ttaaaaaagg gaacggcagc agcataccac gagttaaccg gacatcacac gtaagcctga
    31141 tgcccggttt acgacattaa cgcatcagca gatagatgct ttcattaccg cgtacaattt
    31201 gcagggcgat gatggccggt tttgccgcca gcactttacg catttcagca atcgagttca
    31261 cccgatcgcg gttgacgcca atgatcacat cgtctttttg caagccagcc tgagcagctg
    31321 ggcttccttt gacaacttca tcgatcttaa tacctttgcc gccatctttt agctgaccat
    31381 cgctcaacgt tgcaccttcc agcgctggcg tgatcatttc agcgctggcc gacgaagagg
    31441 tgctggtatc aagcgtcact tctacttcca gtggtttgcc gttacgcagc aggccaagct
    31501 tcactttcgt gcccggctct gtagtcgcga tacgagagcg caactcagca aagctattca
    31561 gcggtttgcc gttgaggctg gtaataatat cgcccgcttt gacgcccgct tttgccgagc
    31621 cagaacctgg caacacttcg ctgacaaacg cgccacgctg cacgtcaagg ttgaaggctt
    31681 tggcgatatc ggcactcatc tcggtgcctt tgatgcctaa caaaccgcgt ttgatttcac
    31741 caaagtcgat aagctgctgc gccagtgttc gcgccatatt actggggatg gcaaatccaa
    31801 tcccgacgct cccgccgcca ggcgcaagga ttgcagtgtt gatgccaatt aactcaccgt
    31861 taaggtttaa tagtgcaccg ccggagttac cgcggttaat ggaagcatct gtctggataa
    31921 agttttccag accttcaaga ttcaacccgc tgcggcctaa tgcggaaaca atgccagagg
    31981 tggcggtttg cccaaggcca aatgggttac cgaccgctac ggcaaaatca ccgacgcgca
    32041 atttatcgga gtcggcaata gcgatttgcg ttaatttgct cgggttttga atttgtaaca
    32101 gggcgatatc gctctggtca tcgctaccaa tcagttttgc atcaaactcg cgcccatcat
    32161 tgagctgaat actgattttc tgtgcctgat taatcacatg gttgttggtc agcacatagc
    32221 ctttactggc gttgatgatg acaccggaac ctaaaccttc gaagggttgt gcaggttgat
    32281 ccggtaaatc atcaccaaaa aactttttga attcttccgg gattttctgt ccctgactgg
    32341 ccgttccttc cacccgtacg ctcaccactg ccggaagcac tttttccagc attggagcca
    32401 gactggggag aggggcctga tcggcaacct ggcctggaat cgacgcgacg gcctgaaatg
    32461 acgccgagag agttaacccg acacttaacg ctaatgcact caacagctgg gtttgttttt
    32521 tcattattcc tgctctcgta cctgaatgat aagaaaagag attcaaaaca gtttgatgtt
    32581 attgaatttt cagacggcta acaatgagac accagattaa atgatagtcg gggagggaga
    32641 gaagaggagg gcgcaatggc tgcgcccgaa aaataaatta gtcgcgcttc gcgccagtac
    32701 gcagcaggcc ggatgcgcct tcagaatagt cgcgaggcat ctgcaccggt gcctgatcgt
    32761 tgctggcttc agactctgcc agacgattac ggaacgggtt tgcttcagca gacagttccg
    32821 gcagcaggct gctggagctt ttcgccatgt gctgatacag ctggcgatag tcgtgcgcca
    32881 tggtatccag taattccgcg ctgcgggcaa agtggctaac cagctcttcg cgatactcgt
    32941 ccagttcagc tttattcttt tccagttcgt actgcaacgc ctgttgctgg cgtagtttac
    33001 gattaccaaa acgcatggcc acagcaccaa taatgatgcc gacgactaac ccaattagcg
    33061 catattccca ggtcatgaac atctcccgtt gtcttttgtt tccgtagggt gttggcttca
    33121 ggctccatgc ctgcggctga ttatgccact ataaccgtta attccacaga agtggaatcc
    33181 cgactgcata tcgcgtagtg tagaacggcc tttttttcgt caaccgtgaa caacggcgca
    33241 ccgattattc aaggaataac aataagatca tgcaaagcgt taccccaaca tcgcaatacc
    33301 tgaaggagct caatgaaggc agccatcaac ccgacgacgt tcaaaaagag gccgtcagcc
    33361 gcctggaaat tatttatcaa gaactcatca atagcaggcc accagccccc aggacgagtg
    33421 ggctaatggc gcgggtcggt aagctgtggg gtaaacgcga agacacaaag catacgccag
    33481 tgcgtggctt atatatgtgg ggcggtgtag gacgcgggaa aacctggctg atggaccttt
    33541 tctatcaaag cctgccggga gagcggaaac agcgcctgca ctttcaccgt tttatgctgc
    33601 gggtgcacga agagctaact gccttacagg ggcagaccga tccgctggaa attattgccg
    33661 atcgctttaa agccgaaact gacgtgctct gttttgacga attttttgtt tctgatatta
    33721 ccgacgccat gctacttggc ggtctgatga aagccctgtt cgcccgcggc attaccctgg
    33781 tagcgacgtc aaatattccg ccggatgaac tttatcgaaa tggcctgcaa cgtgcgcgtt
    33841 ttctgcctgc aatcgatgcc attaaacagc attgtgatgt aatgaacgtg gacgctggtg
    33901 ttgattatcg actgcgtaca ctcactcagg cgcatctgtg gctttcgcca ctcaacgatg
    33961 aaacccgggc gcagatggat aaactatggt tggcgctggc gggggcgaaa cgagaaaatt
    34021 caccgacgtt agaaatcaac catcggccat tggcgacaat gggcgtcgag aaccagacgc
    34081 tggcggtctc ttttactacg ctgtgcgtcg acgcccgcag tcagcatgac tatattgcgc
    34141 tctcacgtct ctttcatacg gtcatgttgt ttgatgtacc agttatgacg cggttgatgg
    34201 agagcgaagc gcggcgcttt attgcgctgg tggatgagtt ttacgagcgc catgtcaaat
    34261 tagtggtgag tgcagaagtg ccgctgtatg aaatttatca gggcgatcgg ctgaagtttg
    34321 agttccagcg ttgcctgtca cgtctgcaag agatgcaaag cgaagagtat ctgaagcgcg
    34381 agcatttggc gggttaaaac ctgtcacaaa tcacaaaaag gggtcgatct ttgaccccga
    34441 cttctctata atcctgcgac cccacgttac aagaaagttt ttttcccaaa actttttgtg
    34501 tgctggcata ggctattcga aggggtaggt ttgccggact ttgtcgtgtg aacctcaaca
    34561 attgaagacg tttgggtgtt caccaacgtg taactattta ttgggtaagc ttttaatgaa
    34621 aacttttaca gctaaaccag aaaccgtaaa acgcgactgg tatgttgttg acgcgaccgg
    34681 taaaactctg ggccgtctgg ctactgaact ggctcgtcgc ctgcgcggta agcacaaagc
    34741 ggaatacact ccgcacgtag ataccggtga ttacatcatc gttctgaacg ctgacaaagt
    34801 tgctgtaacc ggcaacaagc gtactgacaa agtgtactat caccacaccg gccacatcgg
    34861 tggtatcaaa caagcgacct ttgaagagat gattgctcgc cgtcctgagc gtgtgattga
    34921 aatcgcggtt aaaggcatgt tgccaaaagg cccgctgggt cgtgctatgt tccgtaaact
    34981 gaaagtttac gcgggtaacg agcacaacca cgcggcacag caaccgcaag ttcttgacat
    35041 ctaatcggga ttataggcaa tggctgaaaa tcaatactac ggcactggtc gccgcaaaag
    35101 ttccgcagct cgcgttttca tcaaaccggg caacggtaaa atcgtaatca accaacgttc
    35161 tctggaacag tacttcggtc gtgaaactgc tcgcatggta gttcgtcagc cgctggaact
    35221 ggtcgacatg gttgagaaac tggacctgta catcaccgtt aaaggtggtg gtatctctgg
    35281 tcaggctggt gcgatccgtc acggtatcac ccgcgctctg atggaatacg acgagtccct
    35341 gcgttctgaa ctgcgtaaag ctggcttcgt tactcgtgac gctcgtcagg ttgaacgtaa
    35401 gaaagtcggt ctgcgtaaag cacgtcgtcg tccgcagttc tccaaacgtt aattggcttc
    35461 tgctccggca gaaaacaatt tgcgaaaaaa cccgcttcgg cgggtttttt tatggttaaa
    35521 ttctgaatca gcgtaaaaat tggaaagttg ctttttgcta ccgtctgaca gacaggcaaa
    35581 acaaaaacca catcgccaat aagggactaa gtcaactatt tcagactaaa gcgcatctct
    35641 ttttccccat ttccggcatc gactcaccac aaaggtcgca aaatctggta aactatcatc
    35701 caattttctg cccaaatgtc gggtattgct cattttttgt ttgattttcg aacaaagaga
    35761 gtcgttcctt actgggtaac acaacttctg actggccacc tggtggctgg tagcagtaaa
    35821 aattctgact atacctggag gttttcatgg ctgtcgctgc caacaaacgt tcggtaatga
    35881 cgctgttttc cggtcctact gacatctata gccatcaggt ccgcattgtg ctggctgaga
    35941 aaggtgtaag tttcgagatc gaacacgtgg aaaaggacaa tccgcctcag gatctgattg
    36001 acctcaaccc gaatcagagc gttccgaccc tggtggatcg tgagctgacc ctgtgggaat
    36061 ctcgcatcat tatggaatat ctggatgagc gtttcccgca tccgccactg atgcctgttt
    36121 acccggtagc tcgcggtgaa agccgtctgt acatgcatcg catcgaaaaa gactggtaca
    36181 cgctgatgaa caccatcatc aacggttcag cttctgaagc agatgccgca cgtaagcaac
    36241 tgcgcgaaga actgctggcg attgcgccgg tcttcggtca gaagccgtac ttcctgagcg
    36301 atgagttcag cctggtcgat tgctatcttg ctccgctgct gtggcgtctg ccgcaactgg
    36361 gcatcgagtt cagcggcccg ggtgcgaaag agctgaaagg ctatatgacc cgcgtctttg
    36421 agcgtgactc tttccttgct tctttaactg aagcagaacg tgaaatgcgt ctgggccgga
    36481 gttaatctgt atggatttgt cacagctaac accacgtcgt ccctatctgc tgcgtgcatt
    36541 ctatgagtgg ttgctggata accagctcac gccgcacttg gtggtggatg tgacgctccc
    36601 tggcgtgcag gttcctatgg aatatgcgcg tgacgggcaa atcgtactca acattgcgcc
    36661 gcgtgctgtc ggcaatctgg aactggcgaa tgatgaggta cgctttaacg cgcgctttgg
    36721 cggcattccg cgtcaggttt ctgtgccgct ggctgccgtg ctggctatct acgcccgtga
    36781 aaatggcgca ggcacgatgt ttgagcctga agctgcctac gatgaagata ccagcatcat
    36841 gaatgatgaa gaggcatcgg cagacaacga aaccgttatg tcggttattg atggcgacaa
    36901 gccagatcac gatgatgaca ctcatcctga cgatgaacct ccgcagccac cacgcggtgg
    36961 tcgaccggca ttacgcgttg tgaagtaata caaaaacagg ccctggtggc ctgttttgtc
    37021 ttttaatggc ttaataaaat agcgtgattg tggcaatcat attcaccacg aaaccgactg
    37081 ccatggggat cgctgttcgc ttcaccaccg caaaaggcga aacgcctgca attcctgacg
    37141 taacgaccac caccgcagta atcggcgaaa ccgcgcgcgc tagcgtcgtg gcaaaatgca
    37201 tcggcataat cattacaacc gctggtacat gtagtccggc tgcgatattc ggaataagac
    37261 tggcaaatga cataaacggc gcattgccag agcccatcac aatggcacaa atggcaatga
    37321 ctagctccat aataatcatc acgccaatac cgcccagacc agaatgctca gcacccctga
    37381 taaccgcatc aacagtgcca atcgtcgtta agcctttcgc aaatatttca cccgcgacca
    37441 ccagcgttac cacgttggca aactgcgtac ccatgccgtc aaaaaacgcc tgcacatcgt
    37501 ccattgtttc gcgcaagtta tgcttgcgga agaactcaac aaacatcgtc accgtcaaac
    37561 tcagtaacat caccaccacc agatgcagtt ctgactgcat cagcccgatg tgggcgagga
    37621 acagcgagcc gagcatcaag attaacggca tcacaggtaa aatggcgtaa tagagcggcg
    37681 ggacattatc gagagctttt agctctgcct gttcgtgatt gatattttta tcttttttgt
    37741 caaaagcgcg ttgcacgaaa aagtgggaga tcgccaccga gataatgacg caagaggcga
    37801 ccggaagctg gtagtggaag aagtaagtgg caattttcat tcccgcgacc tgggctgcaa
    37861 aaatggagtt cgtttccaga atcccccact caatggacat cgtggttgcg ataaccgcca
    37921 ccgcagagag gcgacttact cccagactca ccagcgtcgg aaataaggtg accatcagca
    37981 acatacccag gccggaggcg ctggtaataa actgcgccat gatttggccg atgacgtaag
    38041 ttgccgacag aataatatac ggcgagtgaa tgagttttaa cgggcggctt agcaagctca
    38101 ccatcgcgcg actggctcct gtgcgctcca tgtagcgggc ataaccgccc accgccataa
    38161 tcgacagccc caatccggca atgcggttgg acagcatgcg caggatttca ttataaatat
    38221 cgacgatcaa gtagccgctg cttttggcgg gatcgagtac accaccgaac cctaaccaga
    38281 caccgcacat catcaggaag atacctgcgg cagccagcac cacctgaggt ttgtagtttt
    38341 tcaggatcaa atagcccatc gtaattaata cgatgacaga tataattatg ccgaacattg
    38401 ttgaactccg tgtcaaaaga aaacggtcaa tcccataaac ggcagattga aaacaatgat
    38461 gttatatttt ttgcaagtct atttatggtg cggatgtcgt gtttttaatt gtag
//
LOCUS       DAZGAN020000038        37396 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGAN020000038 DAZGAN020000000
VERSION     DAZGAN020000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 37396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.038"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(34..330)
                     /gene="yaaX"
                     /locus_tag="ACHKYO_RS10680"
                     /old_locus_tag="pgaptmp_002136"
     CDS             complement(34..330)
                     /gene="yaaX"
                     /locus_tag="ACHKYO_RS10680"
                     /old_locus_tag="pgaptmp_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HGU4428987.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(544..1830)
                     /gene="thrC"
                     /locus_tag="ACHKYO_RS10685"
                     /old_locus_tag="pgaptmp_002137"
     CDS             complement(544..1830)
                     /gene="thrC"
                     /locus_tag="ACHKYO_RS10685"
                     /old_locus_tag="pgaptmp_002137"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGU4428988.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1831..2763)
                     /gene="thrB"
                     /locus_tag="ACHKYO_RS10690"
                     /old_locus_tag="pgaptmp_002138"
     CDS             complement(1831..2763)
                     /gene="thrB"
                     /locus_tag="ACHKYO_RS10690"
                     /old_locus_tag="pgaptmp_002138"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGU4428989.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2765..5227)
                     /gene="thrA"
                     /locus_tag="ACHKYO_RS10695"
                     /old_locus_tag="pgaptmp_002139"
     CDS             complement(2765..5227)
                     /gene="thrA"
                     /locus_tag="ACHKYO_RS10695"
                     /old_locus_tag="pgaptmp_002139"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HGU4428990.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5253..5372)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5308..5373)
                     /gene="thrL"
                     /locus_tag="ACHKYO_RS10700"
                     /old_locus_tag="pgaptmp_002140"
     CDS             complement(5308..5373)
                     /gene="thrL"
                     /locus_tag="ACHKYO_RS10700"
                     /old_locus_tag="pgaptmp_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HGU4428991.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5587..6273)
                     /gene="yjtD"
                     /locus_tag="ACHKYO_RS10705"
                     /old_locus_tag="pgaptmp_002141"
     CDS             complement(5587..6273)
                     /gene="yjtD"
                     /locus_tag="ACHKYO_RS10705"
                     /old_locus_tag="pgaptmp_002141"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU4428992.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6673..6813)
                     /gene="yjjY"
                     /locus_tag="ACHKYO_RS10710"
                     /old_locus_tag="pgaptmp_002142"
     CDS             complement(6673..6813)
                     /gene="yjjY"
                     /locus_tag="ACHKYO_RS10710"
                     /old_locus_tag="pgaptmp_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HGU4428993.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6909..7625
                     /gene="arcA"
                     /locus_tag="ACHKYO_RS10715"
                     /old_locus_tag="pgaptmp_002143"
     CDS             6909..7625
                     /gene="arcA"
                     /locus_tag="ACHKYO_RS10715"
                     /old_locus_tag="pgaptmp_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HGU4428994.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7684..9036)
                     /gene="creD"
                     /locus_tag="ACHKYO_RS10720"
                     /old_locus_tag="pgaptmp_002144"
     CDS             complement(7684..9036)
                     /gene="creD"
                     /locus_tag="ACHKYO_RS10720"
                     /old_locus_tag="pgaptmp_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HGU4428995.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDEPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQCQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTGAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(9094..10518)
                     /gene="creC"
                     /locus_tag="ACHKYO_RS10725"
                     /old_locus_tag="pgaptmp_002145"
     CDS             complement(9094..10518)
                     /gene="creC"
                     /locus_tag="ACHKYO_RS10725"
                     /old_locus_tag="pgaptmp_002145"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HGU4428996.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10518..11207)
                     /gene="creB"
                     /locus_tag="ACHKYO_RS10730"
                     /old_locus_tag="pgaptmp_002146"
     CDS             complement(10518..11207)
                     /gene="creB"
                     /locus_tag="ACHKYO_RS10730"
                     /old_locus_tag="pgaptmp_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HGU4428997.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11220..11693)
                     /gene="creA"
                     /locus_tag="ACHKYO_RS10735"
                     /old_locus_tag="pgaptmp_002147"
     CDS             complement(11220..11693)
                     /gene="creA"
                     /locus_tag="ACHKYO_RS10735"
                     /old_locus_tag="pgaptmp_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HGU4428998.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            11904..12773
                     /gene="robA"
                     /locus_tag="ACHKYO_RS10740"
                     /old_locus_tag="pgaptmp_002148"
     CDS             11904..12773
                     /gene="robA"
                     /locus_tag="ACHKYO_RS10740"
                     /old_locus_tag="pgaptmp_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HGU4428999.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(12770..13417)
                     /gene="gpmB"
                     /locus_tag="ACHKYO_RS10745"
                     /old_locus_tag="pgaptmp_002149"
     CDS             complement(12770..13417)
                     /gene="gpmB"
                     /locus_tag="ACHKYO_RS10745"
                     /old_locus_tag="pgaptmp_002149"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HGU4429000.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            13469..13990
                     /gene="yjjX"
                     /locus_tag="ACHKYO_RS10750"
                     /old_locus_tag="pgaptmp_002150"
     CDS             13469..13990
                     /gene="yjjX"
                     /locus_tag="ACHKYO_RS10750"
                     /old_locus_tag="pgaptmp_002150"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HGU4429001.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(14075..14401)
                     /gene="trpR"
                     /locus_tag="ACHKYO_RS10755"
                     /old_locus_tag="pgaptmp_002151"
     CDS             complement(14075..14401)
                     /gene="trpR"
                     /locus_tag="ACHKYO_RS10755"
                     /old_locus_tag="pgaptmp_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HGU4429002.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(14491..16428)
                     /gene="sltY"
                     /locus_tag="ACHKYO_RS10760"
                     /old_locus_tag="pgaptmp_002152"
     CDS             complement(14491..16428)
                     /gene="sltY"
                     /locus_tag="ACHKYO_RS10760"
                     /old_locus_tag="pgaptmp_002152"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HGU4429003.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            16639..18306
                     /gene="ettA"
                     /locus_tag="ACHKYO_RS10765"
                     /old_locus_tag="pgaptmp_002153"
     CDS             16639..18306
                     /gene="ettA"
                     /locus_tag="ACHKYO_RS10765"
                     /old_locus_tag="pgaptmp_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HGU4429004.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(18613..19845)
                     /gene="nadR"
                     /locus_tag="ACHKYO_RS10770"
                     /old_locus_tag="pgaptmp_002154"
     CDS             complement(18613..19845)
                     /gene="nadR"
                     /locus_tag="ACHKYO_RS10770"
                     /old_locus_tag="pgaptmp_002154"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HGU4429005.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(19866..21248)
                     /gene="radA"
                     /locus_tag="ACHKYO_RS10775"
                     /old_locus_tag="pgaptmp_002155"
     CDS             complement(19866..21248)
                     /gene="radA"
                     /locus_tag="ACHKYO_RS10775"
                     /old_locus_tag="pgaptmp_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGU4429006.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(21297..22265)
                     /gene="serB"
                     /locus_tag="ACHKYO_RS10780"
                     /old_locus_tag="pgaptmp_002156"
     CDS             complement(21297..22265)
                     /gene="serB"
                     /locus_tag="ACHKYO_RS10780"
                     /old_locus_tag="pgaptmp_002156"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HGU4429007.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            22371..23015
                     /gene="ytjB"
                     /locus_tag="ACHKYO_RS10785"
                     /old_locus_tag="pgaptmp_002157"
     CDS             22371..23015
                     /gene="ytjB"
                     /locus_tag="ACHKYO_RS10785"
                     /old_locus_tag="pgaptmp_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HGU4429008.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            23043..24059
                     /gene="lplA"
                     /locus_tag="ACHKYO_RS10790"
                     /old_locus_tag="pgaptmp_002158"
     CDS             23043..24059
                     /gene="lplA"
                     /locus_tag="ACHKYO_RS10790"
                     /old_locus_tag="pgaptmp_002158"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HGU4429009.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSTWIA
                     GAVR"
     gene            24091..24354
                     /locus_tag="ACHKYO_RS10795"
                     /old_locus_tag="pgaptmp_002159"
     CDS             24091..24354
                     /locus_tag="ACHKYO_RS10795"
                     /old_locus_tag="pgaptmp_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4429010.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(24515..25234)
                     /gene="deoD"
                     /locus_tag="ACHKYO_RS10800"
                     /old_locus_tag="pgaptmp_002160"
     CDS             complement(24515..25234)
                     /gene="deoD"
                     /locus_tag="ACHKYO_RS10800"
                     /old_locus_tag="pgaptmp_002160"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGU4429011.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(25314..26537)
                     /gene="deoB"
                     /locus_tag="ACHKYO_RS10805"
                     /old_locus_tag="pgaptmp_002161"
     CDS             complement(25314..26537)
                     /gene="deoB"
                     /locus_tag="ACHKYO_RS10805"
                     /old_locus_tag="pgaptmp_002161"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGU4429012.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(26589..27911)
                     /gene="deoA"
                     /locus_tag="ACHKYO_RS10810"
                     /old_locus_tag="pgaptmp_002162"
     CDS             complement(26589..27911)
                     /gene="deoA"
                     /locus_tag="ACHKYO_RS10810"
                     /old_locus_tag="pgaptmp_002162"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HGU4429013.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQDAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(28038..28817)
                     /gene="deoC"
                     /locus_tag="ACHKYO_RS10815"
                     /old_locus_tag="pgaptmp_002163"
     CDS             complement(28038..28817)
                     /gene="deoC"
                     /locus_tag="ACHKYO_RS10815"
                     /old_locus_tag="pgaptmp_002163"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGU4429014.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            29075..30625
                     /gene="yjjI"
                     /locus_tag="ACHKYO_RS10820"
                     /old_locus_tag="pgaptmp_002164"
     CDS             29075..30625
                     /gene="yjjI"
                     /locus_tag="ACHKYO_RS10820"
                     /old_locus_tag="pgaptmp_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HGU4429015.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            30597..31460
                     /gene="yjjW"
                     /locus_tag="ACHKYO_RS10825"
                     /old_locus_tag="pgaptmp_002165"
     CDS             30597..31460
                     /gene="yjjW"
                     /locus_tag="ACHKYO_RS10825"
                     /old_locus_tag="pgaptmp_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HGU4429016.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(31775..32557)
                     /gene="yjjV"
                     /locus_tag="ACHKYO_RS10830"
                     /old_locus_tag="pgaptmp_002166"
     CDS             complement(31775..32557)
                     /gene="yjjV"
                     /locus_tag="ACHKYO_RS10830"
                     /old_locus_tag="pgaptmp_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4429017.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(32554..33627)
                     /gene="yjjU"
                     /locus_tag="ACHKYO_RS10835"
                     /old_locus_tag="pgaptmp_002167"
     CDS             complement(32554..33627)
                     /gene="yjjU"
                     /locus_tag="ACHKYO_RS10835"
                     /old_locus_tag="pgaptmp_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HGU4429018.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(33749..33910)
                     /gene="ytjA"
                     /locus_tag="ACHKYO_RS10840"
                     /old_locus_tag="pgaptmp_002168"
     CDS             complement(33749..33910)
                     /gene="ytjA"
                     /locus_tag="ACHKYO_RS10840"
                     /old_locus_tag="pgaptmp_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HGU4429019.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(34037..34642)
                     /gene="osmY"
                     /locus_tag="ACHKYO_RS10845"
                     /old_locus_tag="pgaptmp_002169"
     CDS             complement(34037..34642)
                     /gene="osmY"
                     /locus_tag="ACHKYO_RS10845"
                     /old_locus_tag="pgaptmp_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HGU4429020.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(35035..36621)
                     /gene="prfC"
                     /locus_tag="ACHKYO_RS10850"
                     /old_locus_tag="pgaptmp_002170"
     CDS             complement(35035..36621)
                     /gene="prfC"
                     /locus_tag="ACHKYO_RS10850"
                     /old_locus_tag="pgaptmp_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGU4429021.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVQASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVS
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            36840..37088
                     /locus_tag="ACHKYO_RS10855"
                     /old_locus_tag="pgaptmp_002171"
     CDS             36840..37088
                     /locus_tag="ACHKYO_RS10855"
                     /old_locus_tag="pgaptmp_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4429022.1"
                     /translation="MRIEICIAKEKICKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIR"
BASE COUNT         8909 a        10244 c         9373 g         8870 t
ORIGIN      
        1 ctgaatgccg gattgttacc cggcatttgt catttagcgg tgatgtttgc ctggaccatg
       61 accgccgtga tgatcatgag gagctttctt atggtggcgc ggcggtggcg gcggtccgtg
      121 tgggtgccag cgattgcctc gccattcata atgttgtttc caccagccgt ggtcgcgcca
      181 gtgacctcca tcccagtaat agccacgatt atcacgatcg cctatctgta attttactga
      241 cgggactaac gtaatttccg cagcctgtgc tgccatggga gcgaccagaa ccagggaaag
      301 tgcgagtacg atagattgca tctttttcac aggttactcc ttttatcatt gcccgttatg
      361 ggtcgatatc cggagattac gcgatgagaa cgacggttgt tattctccgc aatcctaatc
      421 tctaagttac cgcatttatt gagaatttct cctttttccc tgtcgttttt ctccataatt
      481 tcttcataaa aaagccgggc tgcataaaag caaacccggc ctgattgaga taatgaatag
      541 attttactga tgattcatca tcaatttacg caacgcagca aaatcggcgg gcagattatg
      601 tgaaagcaag ggtaaatcag cacgttctgc cagctctttt ggcagatcca acgtttcacc
      661 gagaatcgct tccacgctct ctttaaattt cgccggatgc gcggtgccga ggaacaagcc
      721 atattcgcct ggattcaact ggtcacgcag cgcacgatac gctacggcag cgtgcggctc
      781 cgaggtgtag cccagttctt ttaactcacg cattgtctgt tgcgtggttt catcatccac
      841 ggctgcataa cccagctctt tcagttgcca gattttgcgg cggaacaact cttccacacg
      901 cggccagttg ttcggctgac tcacgtccat cgcgttggat aacgtcgcct gagtcgcttt
      961 gggtgaccac tgaccgtcgt gcaggaaacg tggcacggta tcgttcacgt tggtcgcagc
     1021 aataaaacgt ttcaccggca gaccgagtga cttcgccagc agacccgccg tcaaatcgcc
     1081 gaagtttccg cttggcaccg agacaaccag ctggttgcgc gcttcctgcg gcagctgcgc
     1141 aacagcttca aagtagtagc aaatctgcgc cagcaaacgg ctgatgttaa tcgagttagc
     1201 tgagtttaac cccagcgcca ctttcagttc ttcatcatca aacgcctgct tcaccagcgc
     1261 ctgacaggca tcgaaatcgc cgtcgatggc aacagtttcg atattgccgc ccaacgtaca
     1321 gaacagtttt tcttgcagtg gactgatttt gcctcgtgga tagaggataa ccactttcac
     1381 attcggtaaa ccgtagaaag catgagccac tgccgctccg gtatcaccgg aggtcgcggt
     1441 cagaatggtc actggcttat cgcccgcaat atgggtcagc atttgtgcca taaagcgacc
     1501 gccgaaatct ttaaatgcca gcgttggccc gtggaacaat tccagacaac cgacatcgct
     1561 ttcaacattg gcgaccggag ccgggaaggc aaacgccgcg cgcacgcgct cttccaggat
     1621 ttcctgcggg atttcatcac caataaacgc cgagaggatc ttcgcactgc gggtgacaaa
     1681 atccagcttc agcatctcat caatttcagt caggctgaat tccggcaggt catgcggaaa
     1741 aaacagcccc tgatttttgc ccaacccctg ggttacggct tgcgcaaagc tgacctgctc
     1801 gttgtgatct ttcagattgt agagtttcat ttagttttcc agtactcgtg cgcccgccgt
     1861 atccagccgg caaatatgaa caaaaccttc ctgattttgc aggtagttct tacccaacca
     1921 gtcggcaacg cgctgggcgg tatccggctt gtcacataga gcgaacaagg tcgggccgga
     1981 gccggagata ccgctcgcta ccgcgccgat ttccgcgacc gcctgccgcg cctgccggaa
     2041 gccaggcagt aaccgttcac ggtagggttc agcgataaca tctttcatca gcttcgcggc
     2101 aagctcaggc tgacgggaat agcaggcgtg aatgaagcca gccagatgtc gcccgtgcgc
     2161 aatgcaatcc tggcggcgat actgcgccgg taaaatagcc ctggcttctg ccgtcgagac
     2221 tttaatcccc ggatacgcca gcacccacag ccactcatca aaccctggca cttgctggct
     2281 gatgatgtcg ttttcttcga tcatcaactg cataccacca agaaaacacg gtgccacgtt
     2341 gtcgtaatga atgctgccgg agattcgtcc ttccagctcg cccatcaaag ccagcaaacg
     2401 agtgtcatta agcggcttgc cacagtgttc attcatcgcc atcagcgccg cgaccaccga
     2461 acaggcgctg gagcctaagc ccgaaccgat tggcatattc ttttccagag tcatcgccac
     2521 tggaatttgc ttgccaagct cctggcaaaa acgctcccag cactgataaa cgatattttc
     2581 ccgtggttct gacggcagct tatcggcaaa gcgtccgagg ttgttgagac tgaatgtctc
     2641 tgccgcctca accgtgacta catctccgag caatgcacca tcaacaggtg tcaccgccgc
     2701 cccgagcaca tcaaacccga cgctcatatt ggcactggaa gccggggcat aaactttaac
     2761 catgtcagac tcctaacttc catgagaggg tacgtagcag atcggcaaag acaccggcag
     2821 ctgtaacgtc attgcccgca ccatatccgc gcagcaccaa cggcagcggc tgataatagt
     2881 ggctataaaa ggccagggcg ttttcgccat ttttcacttt gaacagcgga tcattaccat
     2941 ccacttcggc aatcttcacg cggcaggcac catcttcatc aatattgcca acatagcgca
     3001 aaacttttcc ttcatcacgg gccttcgcca cgcgcgcggc aaagagatcg tcgagctgtg
     3061 acagattcgc cataaaagcg gcaacatcac cctcagcgtt aaactctgcg ggcagcacag
     3121 gttcaatttc aatatccgcc agctccagtt cacgtcccgt ttcacgagcg agaatcaata
     3181 gcttacgcgc tacatccata ccagaaagat catctcgcgg atccggttcg gtataaccca
     3241 tttcccgcgc cagagtagtc gcctcggaga aactcatgcc ttcgtctaac ttgccgaaga
     3301 tataagaaag cgaacctgaa agaatgccgg agaacttcat caattcatca ccagcattga
     3361 gcagattttg caggttctca ataaccggta atccagcccc aacgttggtg tcatagagga
     3421 atttacgccg cgatttttcc gccgcatgac gcaacagatg gtagtaatcc atcgacgagg
     3481 tgttggcctt tttgttcggc gtgacaacgt ggaaaccttc gcgcaagaag tcggcatatt
     3541 gatccgccac tgcctggctg gaggtgcagt caacaatgac cgggttcagc agatgatatt
     3601 ctttcacgag gcgaattaag cgcccgagat taaacggctc tttggcttgc gccagttctt
     3661 cctgccagtt ttccagattt aggccatgca cattggtgag cagtgccttc gagttggcaa
     3721 caccgcagac acgtaagtcg atatgtttat tcttcagcca gctttgttga cgcttcagtt
     3781 gctccagcag cgcaccgcca acgccaccga cgccaatcac aaacacttcg ataacctgat
     3841 cggtattgaa cagcatctga tgagtaacgc gcacgccagt ggtcgcatca tcgttattta
     3901 ccacgacaga gattgagcgt tcagaagatc cctgagcaat ggcgacaatg ttgatattgg
     3961 cgcgggccag cgcggcaaag aatttcgccg agatcccacg caaggtgcgc ataccatcac
     4021 ctaccaccga gataatggcc agccgttccg tcaccgccag cggctccagt aagccttctt
     4081 tcagttccaa gtagaactct tcctgcattg cccgttcagc ccgcacacag tcgctttgcg
     4141 gaacgcagaa actgatactg tattcggaag atgattgcgt aatcagcacc acggaaatac
     4201 gggcgcgtga catcgcggca aagacgcgcg ccgccatgcc gaccattcct ttcatccccg
     4261 ggccggaaac gctgaacatt gccatgttat tcagattgga aatgcccttg accggtaatt
     4321 cgtcttcatc acgactggca ccaatgagcg tacctggtgc ttgaggattt ccggtatttt
     4381 taatcaggca agggatctgg aactgggcga tgggggtaat ggtgcggggg tgaagaactt
     4441 tagcgccgaa gtaggaaagc tccatcgctt cctggtagga catcgacttc aacaacctcg
     4501 catcgggcac ctgacgcggg tcgcaggtat agaccccgtc aacgtccgtc caaatctcgc
     4561 aacaatcggc gcgtaaacag gcagccagca ccgccgcgga gtagtcggaa ccgttgcgtc
     4621 caagtaccac cagttcgcct ttttcattac cggcggtgaa acctgccatc aacaccatgt
     4681 gatcagccgg aatgcggctt gccgcaatac ggcgggtgga ctcagcaata tcgacggtag
     4741 attcgaggta atgccccact gccagcagtt tttcgaccgg atcgataacg gtaacgttgt
     4801 gaccacgcgc ttctaacacg ccggccataa tggcgatcga cattttctcg ccacggcaaa
     4861 tcagcgcagc gttgatgcta tccgggcact gccccaacaa actaatgcca tgcaggacat
     4921 gttttatttg ggcaaattcc tgatcgacga aagttttcaa ttgcgccagc gggaaccccg
     4981 gctgggcggc ggcgagtccc gtcaaaagtt cggcaaaaat acgttcggca tcgctgatat
     5041 tgggtaaagc atcctggccg ctaatggttt tttcaatcat cgccaccagg tggttggtga
     5101 ttttggcggg ggcagagagg acggtggcca cctgcccctg cctggcattg ctttccagaa
     5161 tatcggcaac acgcagaaaa cgttctgcat ttgccactga tgtaccgccg aacttcaaca
     5221 ctcgcatggt tgttacctcg ttacctttgg tcgaaaaaaa agcccgcact gtcaggtgcg
     5281 ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg taatggtgat
     5341 ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg taatttttat
     5401 ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc aataaaattt
     5461 taatttactc acggcaggta accagttcag aagctgctat cagacactct ttttttaatc
     5521 cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata cttactaagg
     5581 cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca acattgccgt
     5641 gtctcgttgc tctaaaagcc ccaggcgttg ttgtaaccag tcgaccagtt ttatgtcatc
     5701 tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt gcagttgatg
     5761 ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta attgatagca
     5821 atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca tcggcacacc
     5881 agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt cgcgaccaaa
     5941 caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca ccagttctac
     6001 tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga cagtgaaatc
     6061 gacatcgtgt aacgattcag ccagcgtcgg gaaaacttta atattatcaa taatatcacc
     6121 agatccatgt gcgacccagc gggtggctgg ctccaggtgc gcttgactat cgacaatccg
     6181 cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat tttctgctct
     6241 ggcgggtgcg accagaataa tcgttatacg catattgcca ctcttcttga ccaaataacc
     6301 gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat tattaacgta
     6361 agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc tgactgtact
     6421 aacggttgaa ttgttaaaaa atgctacata tccttctgtt tacttaggat aattttataa
     6481 aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc aactagttgg
     6541 attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat caaactgtta
     6601 acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg gtgtccggtc
     6661 cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt ttgacactgt
     6721 cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt tgatggaaag
     6781 tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt aatttgcagc
     6841 atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg gcaatttagg
     6901 tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta acacgcaaca
     6961 cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca gatggcgcgg
     7021 aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat atcaatctgc
     7081 cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat gttgcgttga
     7141 tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa atcggtgcag
     7201 atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca cgcaacctgc
     7261 tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt gaaagctaca
     7321 agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct gatggcgagc
     7381 agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa aacccaggca
     7441 aaattcagtc tcgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg aaaccgcacg
     7501 accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct acgccggata
     7561 cgccggaaat catcgccacc attcacggtg aaggttatcg cttctgcggt gatctggaag
     7621 attaatcggc tttaccaccg tcaaaaaaac ggcgcttttt agcgccgttt ttatttttca
     7681 accttatttc cagatacgta attcatcgtc cgttgtaact tctttactgg ctttcatttt
     7741 cggcagtgaa aacgcatacc agtcgatatt acgggtcaca aacatcatgc cggccagcgc
     7801 caccaccagc acactggttc ccaacaacag cgcgctatcg gcagagttga gcagtcccca
     7861 catcacacca tccagcaaca acagcgcgag ggtaaacaac atgctgttgc gccagccttt
     7921 caataccgcc tgcaaataaa taccgttcat taacgcccca atcagactgg cgattatcca
     7981 tgccccggta aaaccggtat gttcagaaag cgccagcaag agcaaataaa acatcactaa
     8041 tgaaagcccc accagtaaat attgcattgg gtgtaaacgt tgcgcggtga gcgtttcaaa
     8101 aacaaagaac gccataaaag tcagcgcaat cagcagaata gcgtacttag tcgcccggtc
     8161 agttaattgg cattgatcgg ctggcgtcgt cactgcgacg ctaaacgcgg ggaagttttc
     8221 ccagccggta tcattgcctg aagcaaaacg ctcaccgaga ttattagcaa accagctgct
     8281 ttgccattgc gcctgaaaac ctgactcact aacttcccgt ttggctggca aaaaatcacc
     8341 taaaaaactg ggatgcggcc agttgctggt taaggtcatt tcgctattac gcccggcagg
     8401 caccacagaa agatcgccgg taccgcttaa attcagggcc atattcagct tcaggttctg
     8461 cttgcgccag tccccttcag gtaaagggat atgcacgccc tgcccgcctt gctctaaccc
     8521 ggtgccgggt tcaatggtca gcgccgttcc gttaacttca ggcgctttca ccacaccaat
     8581 accacgcgca tccccgacgc taatcacaat aaatggcttg cctaaggtga tatttggctc
     8641 gtcgagttcg ctaagacgcg aaacatcgaa atcggctttt agcgttaaat cactgtgcca
     8701 gacctgaccg gtataaatcc ctatcttgcg ttcttcaacg ttctgattgc catcaaccat
     8761 caatgactca ggtaaccaaa aatggataaa acttcgtttc cgctccacgg ttttatcgtc
     8821 ttcctgcacc gtataaagct cggtcacagg aatagcgatg agcggcccaa cgagtttttg
     8881 cggcccgctg gtactttgcc gaatcgcatc ttccacatcg ctacggtaat cggcacgttc
     8941 gacaatcact tgccgaatca gcattatcgg aatcaacaac agcaatactg caccaaacaa
     9001 ggtggtcatt ttccagaaca ggggggattt caacatagtc ttctcctttg caatggcagc
     9061 aggatacgaa ggctatgtgg gaagaatttg aagctatgtg aagtgacggt gaagtcgaag
     9121 cgaggccagc acgccacctt cctgcacgtt gcgcagcgtg acttcgccgt taaacaaacg
     9181 ggcgacctca ctgacgaacg ccaaccccag accgctgctt ttttgcccat ttgcacgcgg
     9241 caaagagtaa aagcgttcaa aaatacgtga cagcgcgtag tcaggaatac cactaccggt
     9301 atccagcacc ttaagggcga cgtgttcctg atccacttcg gcgcttagcg ttatgcgacc
     9361 gctctcgggg gtaaaatcga tggcgttatc cagtaaattc cccagcgcct gctccagtaa
     9421 cgccggttca gcagcaacat taacctcagt gggcataaca tgtagagtga tgtttttttc
     9481 tgccaactgc acggtgcgcg cttcgctgac gcggcgaaat aatgccgcca catcaacaac
     9541 agtcagaacg acttcctgac gattctccag tcttgcctgg cgtagtaacg tttctaccaa
     9601 tgcctgcata cgcgcatttt gcgtcagaat gttgtcggta aaacgagcca ccacttccgg
     9661 tggcggacct tcgcgtaaaa tttccgccgc gccacgaatc gccgccagtg ggctttttag
     9721 ctcatgggtt aacgcataaa catactgctc aatatagttt ttcccttcca gcttcacgcg
     9781 catactttcc agcgcctgcg caagcttacg caactcgcta ctgccgagtt caggaagagg
     9841 aacgggctta ttgtcagtga cggaatcggc atagcgagtg agccgggcaa tggagcggtt
     9901 gatccaccaa accatgcctg cgccaatcac cagtgcaatc cccagcaaaa tggcgctggc
     9961 ccataaaatt cgccgctcgc tacgcttaat gaccggagcc atcgccgcgt tcggtttgcc
    10021 tacgctcaaa acgccaataa gccgcgagcc gtccataatc ggcgcggcaa catacatcac
    10081 cgaactttcg ggatcggcag gattttgcaa cgtgctgcgc gcaccatact gaccacgcaa
    10141 cgttagccag acgtcattcc agcgcgaata atcctgccca acggctttat ttgccgaatc
    10201 gaacaatact ttaccctgcg aatcggtcat atagacatga tattcattgc gcactttgtt
    10261 aatgccaccg atattggcgc gaaacgggcg atgttgtagc tgattaaacg cctgcgccag
    10321 ttgcccatgc gttgggtccc cagagagcaa atcgggacgc gccagctccg ccagcaacgt
    10381 tgcggtatcg attaacgtcc cctccgttgc tcttcgcacg cccggtttaa cttctttgac
    10441 aaaaatagcc agtacgaacc aggccgccac cgccaccagt aaaaaatagc ccagcagcaa
    10501 ccgcatgccg atacgcatta caggcccctc aggctatatc ccatgccgcg atgagtatta
    10561 atcggtgaaa gatcggggtt gatggcgcgc agcttggcac gcaacgtttt aatgtgggta
    10621 tcaacggtgc gatcgtaggt gtcctgcgca tcttcccaca cgctatccat cagttgctgg
    10681 cgggaccaga cgcggcctgg cgacttgagt aacgtcttca gcaataaaaa ctcataccgc
    10741 gtcagcgtta atggcgtgtc aaaccagctg atctgcgccg cgggttcatt caattcaaaa
    10801 tgaccaatac ggatgacggg agacggcgac gagaacttct tcacccgacg cagtaaggtg
    10861 cgcaccctgg cgcacacttc gcggggtgaa aacggtttag ccacatagtc gtcagcgcca
    10921 atttccagcc caagcaggcg atcgacctct tcacttcggg ccgtcaggaa cagtacaggt
    10981 aacgccggat ggagcgccag taactggcgg cataattcaa agccgctgat atcaggcaaa
    11041 ccaacatcga gaatcatgac gtcgggaacc tgctggcgag ctttatccag caccggcaag
    11101 cctcgctcaa agacctcgac ggcaaaacct tcctgctgca acatgtagac tagcgtgtcg
    11161 gctatccctt gctcatcttc cactaaccag accgtttccc gttgcataaa tcgcctctgt
    11221 tattgccgcc acggcatgac aggaaccgcg ctaatcgcgt ttttcggcga accttctaca
    11281 actttgtcgg agtaagccag ataagcgagc gcgttgcgtt tggcgtcata aaagcgcacg
    11341 acctgtaacg acttaaagac cagggacgtg cgttttttga atactacctc gccctgagct
    11401 ttgccgtttt taatacgatc cgacagttca atcggcccga cttgctgaca agaaatagcc
    11461 gcatcggagg tatcttccgc cagacccaat ccccctttaa taccacctgt tttcgcccgg
    11521 ctcacataac aggtgacatt tttcacatcg ggatcgtcaa aggcttccac aacaattttg
    11581 tgatccgggc cgatcatttt aaatacggtg tcgaccgaac caatctcttc tgcatgagcc
    11641 aatggcccca gcattatcag gcttaaagac aggatcaaat gcttgtattt catattgtta
    11701 ccatttttat ctattacacg agcaattagt tcgtcacggt aaataacata ttgattaaga
    11761 acaaaatctc aatactttta tttccgtcaa gccctaaaac atactctact aagaaaaaaa
    11821 cactgaatgc taaaacagca aaaaatgcta ttatccaatt atctgatgtc aggtgctcgt
    11881 tgttgaaagg atgaggatat tttatggatc aggccggcat tattcgcgac cttttaatct
    11941 ggctggaagg tcatctggat cagcccctgt cgctcgacaa tgtagcggcg aaagcaggtt
    12001 attccaagtg gcacttacag agaatgttta aagatgtcac tggccatgct attggcgcgt
    12061 atattcgtgc tcgtcgtttg tcgaaatcgg cggtcgcact acgcctgact gcgcgtccga
    12121 ttctggacat cgcgctgcaa taccgcttcg actctcaaca gacatttacc cgcgcattca
    12181 agaagcagtt tgcccagact cctgcacttt accgccgttc tcctgaatgg agcgcctttg
    12241 gtattcgccc gccgctacgt ctgggtgaat tcacaatgcc agagcacaaa tttgtcaccc
    12301 tggaagatac gccgctgatt ggtgttaccc agagctactc ctgttcgctg gagcaaatct
    12361 ctgatttccg ccatgaaatg cgttatcagt tctggcacga ttttctcggc aacgcgccga
    12421 ccattccgcc agtgctctac ggcctgaatg aaacgcgtcc gagtcaggat aaagacgacg
    12481 aacaagaggt attctatacc actgcgttag cccaggatca ggcagatggc tatgtactga
    12541 cggggcatcc ggtgatgctg cagggcggcg aatatgtgat gtttacctat gaaggtctgg
    12601 gaaccggcgt gcaggagttt atcctgacag tatacggaac gtgcatgcca atgctcaatc
    12661 tgacgcgccg taaaggtcag gatattgagc gatactaccc ggcagaagat gccaaagcgg
    12721 gagatcgccc aattaatctg cgctgtgaac tgctgattcc gatccgtcgt taacgctgca
    12781 actcatctaa tgcaggggcg tccagatgcg agatgtcccc tgcggtttca acaacccagc
    12841 cagacgccag ccacaggctt tcctgataat ccacgcgcga aatagaacag ttacgcagac
    12901 gtaagcggcg ctctgcccat gctggtaatc cgagaatcgt actcaccaga catcccagtg
    12961 caataccatg gctcaccagc agcggtcggc ttccctgcgg taagtcccgg caagattcca
    13021 gtgccgcatt gacgcgatcg ctgagctcct gcattgactc gccttcagga atacgcccgt
    13081 caacggtgcc attgaccagc tgccgacgcc agttctcttc ttcttcggtc agagaatcga
    13141 tatgtctttt ttccagcaca cccatgttta attcacgcag gcgagaatca aagatgatgt
    13201 cacagccgca ggcctgggcg atgatttccg ccgtacgccg ggtgcgtcct aaatcgctac
    13261 tgataatatg cgtaatgcca agctctttgg cacgggttgc cacctgcatc gcctgttgct
    13321 cacctttggc ggtcagcggg ctgtcagact ggccctgaat acgtcgctcg gcgttccact
    13381 gcgtttcacc gtggcggact aggtatacct gtaacatgct ttttttccgt atactgctgt
    13441 aacgttaatt ttctgagtaa tgctgattat gcaccaagtt gtctgtgcga ccaccaatcc
    13501 cgctaaaatt caggccattc tgcaggcatt tcacgagatc ttcggcgaag gatcctgcca
    13561 tattgcatcc gttgccgtcg agagcggtgt accggaacag ccctttggca gtgaggaaac
    13621 gcgcgctggc gcacgaaatc gggtagcaaa tgcccgccgt ttacttccag aggctgattt
    13681 ttgggtggcg attgaagctg gcatcgatgg tgacagcact ttcagttggg tggtgattga
    13741 aaacaccagc cagcgcggcg aggcgcgttc ggctactttg ccgttacctg cggtgattct
    13801 gcagaaagtg cgtgaaggtg aggcgcttgg tcccgtgatg tcgcgttata ccggtattga
    13861 tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc gccgggaaac tcactcgcgc
    13921 cagcgtctat caccaggcgg tgatcctggc gctgagtccg tttcacaatg ccgtgtatca
    13981 ggcattgtag gacggataag gcgttcacgc cgcatccggc attcggtgca cgatgcctga
    14041 tgcgacgctg ccgcgtctta tcaggcctac aaaatcaatc gcttttcagc aacacctctt
    14101 ccagccactg gcgcagctcc acgggcgcgg ctttcaggct gttagatcca cgcgtaatcg
    14161 tcgcgatgcc cgcgccgagt tcatttttta actcacgctg gctcatttcg ccgcgcaaca
    14221 gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc gtcagcatca
    14281 gatttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg tcgacaaaac
    14341 gtaaccactc ctggtgacgc tgttctgcca tcgctgctga atagggtgat tgttgggcca
    14401 taatatgtcg ccattgttag cgggggaagc aaaatgcctc ccccggttgt actcgctaaa
    14461 gagtacgata gcatatcata aacgtgcgga tcagtaacga cgtccccatt ccgtggcgct
    14521 catcaacgtc ggtttatccc ccatgaaata gcggtagtaa gcgtcataag ccagcacgtt
    14581 cttcacataa ccgcgcgtct cggagaacgg aatactctcg acaaatgcca cagcgtcgat
    14641 acgcccggcg ctgttgccaa gccaggttcg cacccgtcca ggtccggcgt tataagctgc
    14701 tgaggagaaa atacggttat tgccaaactg ctgataaaca tattgcaggt aactggtgcc
    14761 aatgttgata ttcgtttccg gatccagcaa ttgcccaggg ctgctataac caggaataga
    14821 gaacatcttc accgtatggg tcgctgtacc aggcataatc tgcatcaggc cgctggcccc
    14881 taccggtgat ttcactttcg gattccaggc gctctcctga cgggcaatcg ccatcgcata
    14941 gctttgcggg atctctttcc cactggtgta acgtttgaaa agatcgttgt aagccagcgg
    15001 gaatcgctct tccagatgat cccaaagctt cccggcgatc gttgcctgaa cgctaagatc
    15061 ccaccattgg ttattgaaag cataccgagc cagttgagcc tgctctgttt ttgacttgct
    15121 cttcaccaga ttggcccact cgctacgcgc ggtgttatcg agattccagt acatcaactc
    15181 gcgcacgcgc gccatctccg gcccctgagt cagggcgctg tcaacattct gcggcgcttt
    15241 atcaatcttc agctcatact cttctccgat gcgttgtgct gcaaccatcg ggtagaaacc
    15301 acgctgttgc atgagttgat gcaaaatctc tttcgcttca gcttcacgtc cgcgttccag
    15361 caataaatcc gcctgccagt aacgccattc atctttctct tttgcttcca tcggcagacg
    15421 cgccagccag gtattcaggc cgcgacgatc gccggtgcca agtgccattc gcacacggcg
    15481 ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca tcgcgccatt tcgcctgctc
    15541 gtcggtgaca tcgttgccca tcaaacgcca ggcgacgata tcgcgcagct cctgaatctg
    15601 atcttcatta agctgctgcg cctgggcaag cgacgggatc atcagccgtg cattttcagc
    15661 atcctgccgc gccacactgg caaacgccac cgccgccatt tgacgggtaa aatcggtcgc
    15721 tccggttgta cgcgcgaagg tcagtaccgt attagggttg ttcgccagtg aaatgattgc
    15781 cgaggcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta ccaggccagt
    15841 gttacccgct ttcatcgcca gacggatacg ctctaaatac gccagcggat cttgtttacc
    15901 tgacgcacgc cagacgctaa ataacttgtc acaggcgtta ggctggctct tgccggttag
    15961 ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc atttcgcata
    16021 gtagtaatta cattgcgctt cggtagttcc gggcttttcc gggctaaagg ctaacaagcc
    16081 acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg tgcgagcggg
    16141 aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt gattcatcag
    16201 atcatcggtg atctggcggt attccaggta gggataaagc ggataatcct tcagtccagg
    16261 catcatttgt tcgaccacat ccatttgtcg attatcccag gcctgcttga tttgcgcgta
    16321 acggctacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg tcagcagaca
    16381 gacaccggca gccaacagcc gccaggtaac ttgtttggct ttttccacaa gcgcatcctc
    16441 taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct aaccagtcat
    16501 tgccgtttac gccacgttac ggacactttt ttacttttac tgcgaaggtg atcgaccaac
    16561 tgggattagg cggggaaaaa ggctacactt cgcctttgaa aactcatcat cacgataaac
    16621 atagaggcga agtccaacgt ggctcaattc gtttatacca tgcatcgtgt cggcaaagtt
    16681 gttccgccga aacgtcatat tttgaaaaac atctctctga gtttcttccc tggggcaaaa
    16741 attggtgtcc tgggtctgaa cggcgcgggt aagtctaccc tgctgcgcat tatggcgggc
    16801 attgataaag acatcgaagg tgaagcgcgt ccgcagccag acatcaagat tggttacctg
    16861 ccgcaggaac cgcaactgaa cccggaacac accgtgcgtg agtccattga agaagcggtt
    16921 tctgaagtgg ttaacgccct gaaacgcctg gatgaagtgt atgcgctgta cgccgatccg
    16981 gatgccgatt ttgacaagct ggccgctgaa caaggccgtc tggaagagat cattcaggct
    17041 cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg cggatgcgct gcgtctgcca
    17101 gattgggacg cgaaaatcgc taacctctcc ggtggtgagc gtcgtcgcgt ggcgttgtgc
    17161 cgcctgcttc tggaaaaacc agacatgctg ctgctcgacg aaccgaccaa ccacctggat
    17221 gccgaatccg tggcctggct ggaacgcttc ctgcacgact tcgaaggcac cgttgtggcg
    17281 attactcacg accgttactt cctcgataac gttgcaggct ggatcctcga acttgaccgc
    17341 ggtgaaggta ttccgtggga aggcaactac tcctcctggc tggagcagaa agatcagcgc
    17401 ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt cgatcgagaa agagctggag
    17461 tgggtacgtc agggaactaa aggccgccag tcgaaaggta aagcacgtct ggcacgcttt
    17521 gaagagctga acagcaccga atatcagaaa cgtaacgaaa ccaacgaact gtttattcca
    17581 cctggaccgc gtctgggcga taaagtgctg gaagtcagca acctgcgtaa atcctacggt
    17641 gatcgcctgc tgattgatga cctgagcttc tcgatcccga aaggggcaat cgtcgggatc
    17701 atcggtccga acggcgcggg taaatcgacc ctgttccgta tgatctctgg tcaggaacag
    17761 ccggacagcg gcaccatcac tttaggtgaa acggtgaaac tggcatcggt tgatcagttc
    17821 cgtgactcaa tggataacag caaaaccgtt tgggaagaag tttccggcgg gctggatatt
    17881 atgaagatcg gcaacaccga gatgccaagc cgcgcctacg ttggccgctt taactttaaa
    17941 ggggttgatc agggtaaacg cgttggtgaa ctttccggtg gtgagcgcgg tcgtctgcat
    18001 ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc tcgacgaacc aaccaacgac
    18061 ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc tggagttccc gggctgtgcg
    18121 atggttatct cgcacgaccg ttggttcctc gaccgtatcg ccacgcacat cctggactac
    18181 caggatgaag gtaaagttga gttcttcgaa ggtaacttta ctgagtacga agagtacaag
    18241 aaacgtacgc tgggcgcaga cgcactggag ccgaagcgta tcaagtacaa gcgtattgcg
    18301 aagtaatgcg taaaatgccg gatgcggcgt gaacgcctta tccggcctac aaaccaagaa
    18361 atttcaatac gttgtgaatc tccaggcctg ataagacgcg gtaagcgtcg catcaggcat
    18421 tgagcaccga atgccggata cggcgtgaac gccttttccg gcctacaaca gccttagtat
    18481 ccgcaaattc gtagcgggtc acccgtgctg attcttttaa accttgttta atctcgacag
    18541 cctcttccag agaggttttc aactcatcaa aaaacttatt ttatacctcg cctttgagcc
    18601 gtttcatcgc ggttatctct gctcccccat catctcccgc accagctcca cgcagcgcag
    18661 gaaacggctg tcataatcgt cctcttcaac ccgcacgaat tcgatattgt tctcttccag
    18721 catctccacc agcaagttct ggaactcttt gcgatccacc gaactgccga ggctgcgtaa
    18781 accatccgcc acccacggcg tgttgttctc cagcaggatc accagatcga aacggtattc
    18841 atcaatcaac gcctgtacga acggatgctc acgcccttcg tactttttgc agaacgcctg
    18901 ggtagtgaca aaatcagtgt caataaacgc cactttattg gcatatttca ctgcaaaatc
    18961 aatgtattgt gcgtggccca gcgcgatttt atcgtaatcg gaatactgca atgcgatctc
    19021 atcaccgccg aggtgtgaaa agacataatc gcgaccatat tcccacgcac tggtggtatt
    19081 gaagatattg gcaagtttgt ttaccaaagt ggatttaccg ctcgactcgc caccaaggat
    19141 cgccacggta cgtacaaaga acggcttcac ttccgtagga atatattccc agtagcggaa
    19201 cgggttttcg cggatctgcg caccgctgat actcataaag gtacgtttcg gatcgaccag
    19261 caccgtatcg atccccagat gttccatata ctgtggcgca tcggcttctt ccgaggtgta
    19321 gatcaggtcc ggctgaatcc ccttttcagc cataaacttt ttgatgccgt tgctccacac
    19381 atcccagccg tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa tgcgaatatt
    19441 tttctgatat ttaaaagttt gtaataacca gcgcagacga tccggcaccg tgggctgctg
    19501 cgacatggcg ctgtcttcaa acaacgcgcg gtcgcgggta tcgtcaaaac ccataatgat
    19561 atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc cggtatgcag
    19621 cgggtagaac ttaccgaata cgacaccaat ggttttcttc tgtcggggaa attcaagccc
    19681 caaaaaacgg tgcaacgcct ccagcttttg cgcgctgggg cttttaattt tggcattcag
    19741 taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct gtagcgtgca
    19801 gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc cctccgtatc
    19861 tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac gccaaaaatc
    19921 tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc ccggcgaaaa
    19981 ccgtgtttcg ccgcttctga aattcgttcc tgaccgctgg gcaccgggcg gatctcccct
    20041 gccagcccga cttcaccaaa caccaccaga tcctgtggca gcggtctgtc acgcaggctg
    20101 gaaaccatcg ccagcagtaa cgctaaatcg gcactggttt cggttacctt cacgccgccg
    20161 accacgttca caaatacatc ctgatcggcc atttgcagac caccgtgacg gtgcaagaca
    20221 gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg cggattcgcc
    20281 atcatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt tccttcccac
    20341 accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa aattgccgaa
    20401 gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag ctcattcacc
    20461 gcgccgaagc ggtttttatg gctgcgcaag gtgcgaaaac gggagtcggc atcaccatcc
    20521 agaagcaccg aacagtcgat acagtgttcc agcactttag ggccagccag cgagccatct
    20581 ttggttacgt gcccaaccat gacaatcgcc acaccgcgcg ttttggcgaa gcgcgtcaga
    20641 taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgattgtac atccgccata
    20701 tgcatcacct ggatcgagtc aattaccatc agtttcggtt gctcttcttc ggcaatcagg
    20761 cagatctgtt cgatgctggt ttccgacaac atattgagat tgtcagtcgg caggccaagg
    20821 cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cagtgacata cagcgttttc
    20881 atctgctggg ccagtttgca cagcgtttgc agtagcagcg tggatttccc cgcacctggg
    20941 ttaccgccaa tcagaatggc gcttcctggc accacgccgc cgcctagtac gcggtcgaac
    21001 tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga gagtttctgg
    21061 acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg cgccaccgtt
    21121 ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc actgcactgc
    21181 ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg ttttggagct
    21241 tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatggcgggc gagcaattac
    21301 ttctgattca ggctgccaga gaggatgcag aataccccca tcaggtcagc gtgacggatg
    21361 gtgacttccg ccttttcatt cacttttggc ttggcatggt aggcaatccc cagccctgct
    21421 gctttgatca tcggcaggtc attagctcca tcgccaatcg ccacggtctg cgccagcggg
    21481 atttcatact cctgcgcgag gcgagtcaaa gttttcgctt tgtactgcgc gtctacgatg
    21541 tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt ggctaccacg
    21601 gcggtcaggc gcagcttgtc gcgcagatat tcagcaaaga aagtaaagcc gccggaggca
    21661 atcgccactt tccagcccag cgtttccagc ttgagcacca gttgcgttaa gcctggcatc
    21721 agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt cagcgtcgcc
    21781 acacggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg ttcggttact
    21841 tccgccacca tctcgcccgt tccggccagt ttggcaattt catcaataca ttcaatctgg
    21901 atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg cgggattttc
    21961 cccagcgggg cgacatccag ttgcgcttca tgggccaggc gcgtagcccg tgcggtgagt
    22021 gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac aatcaccatc
    22081 gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc acgaccatac
    22141 agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc accacttaat
    22201 gaaagaggca gaccaggcca taaagagaca tcttcaggca ggtcgcacca ggtaatgtta
    22261 ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga ggctaccttg
    22321 tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag atggctcgca
    22381 caaaactgaa attccggctg catcgggcag tgattgtcct gttctgtctt gccttgttag
    22441 tggcgctgat gcagggagcg tcatggttta gtcaaaacca ccagcgacaa cgtaatccac
    22501 agctggaaga actggctcgc accctggcgc gtcaggtaac gctgaacgtt gcaccgctga
    22561 tgcgtaccga ctcaccggat gaaaaacgca ttcaggcgat cctcgatcag ttaacggatg
    22621 aaagccgtat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc gcacgttctg
    22681 gcgaaagcgt cgaagtgcgc gaccggctgg cgctcgacgg taaaaaagca ggcggctatt
    22741 ttaaccagca gattgtcgag ccaattgcgg gtaaaaacgg accgctcggc tatctgcgcc
    22801 tgacactcga cacccatacg ctcgccaccg aagcccaaca ggtggataac accactaaca
    22861 ttttacgcct gatgttgctg ctctcactgg caatcggcgt agtgctgacc cgcacgctgc
    22921 tacagggtaa acgcacccgc tggcagcaat cgcccttcct gttaaccgcc agcaaaccgg
    22981 tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga aaggaaatcg
    23041 ttatgtccac attacgcctg ctcatttctg actcttacga tccgtggttt aacctggcgg
    23101 tggaggaatg tatttttcgc cagatgcccg ccacgcagcg cgttctgttt ctctggcgca
    23161 atgccgacac ggtagtgatt ggtcgcgcgc agaacccgtg gaaagagtgt aatacccggc
    23221 ggatggaaga agataacgtc cgcctggcgc ggcgcagtag cggtggcggc gcggtgttcc
    23281 acgatctcgg caatacctgt tttaccttta tggctggcaa gccggagtac gataaaacca
    23341 tctctacgtc gattgtgctc aatgcgctga acgcgcttgg cgtcagcgcc gaagcgtccg
    23401 gacgtaacga tctggtggtg aaaaccgccg aaggcgaccg caaagtctca ggctcggcct
    23461 atcgcgaaac caaagatcgt ggcttccacc acggcacctt gctgctcaat gccgacctta
    23521 gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa ggcattacct
    23581 cagtgcgttc ccgcgtgacc aacctcaccg agctgctgcc ggggatcacc catgagcagg
    23641 tttgcgaggc cataaccgag gcctttttcg cccattatgg cgagcgcgtg gaagcggaaa
    23701 tcatctcccc ggacaaaacg ccagacttgc caaacttcgc cgaaaccttt gcccgccaga
    23761 gtagctggga atggaacttc ggtcaggctc cggcattctc gcatctgctg gatgaacgct
    23821 ttacctgggg cggcgtggaa ctgcatttcg acgttgaaaa aggccatatc acccgcgccc
    23881 aggtgtttac cgacagcctc aaccccgcgc cgctggaagc cctcgccgga cgactgcaag
    23941 gctgcctgta ccgcgcagat atgctgcaac aggagtgcga agcgctgttg gttgacttcc
    24001 cggaacagga aaaagagcta cgggagttat cgacgtggat agcgggggcg gtgcggtaac
    24061 tcgctgatta aaatatccag ggaaggaaat atgatcgatc tggaaaacca ggaacgggag
    24121 attatcaatt taatgttcag ccagcgtatt tcctggctag ccgccgtgcg tatcaggcat
    24181 aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct cgattaattc tctaaaacag
    24241 atcgaaaaga cggaacggct tagctcaaac atcaaaagca aaatggcaga aatttatggt
    24301 tgtccaccag agttgctgat atgcccttcc tggatgacgg ctgaacataa atgaatagat
    24361 tcgtgaatag atacttgaat gtattttatt ttctatccat atgaaaaata atagtaaatc
    24421 tataaatcag ttgctattga tgaatagata ctaaaaagcc ggagcagtcc cccactccgg
    24481 cttaacacga caatcgcctt tcagcgaaac acaattactc tttatcgccc agcagaacgg
    24541 attccagtgc gattttgatc atgtcgttga aggtggtctg acgctcagcg gcagtggtct
    24601 gctcgtgagt gcggatgtgg tcagacacgg tgcagatggt cagggctttc gcgccaaatt
    24661 ctgcagcgac gccgtagata ccagccgctt ccatttccac gccgaggatg ccgtattttt
    24721 ccatcacgtc gaacatttcg ccgtccggag agtagaacag gtcagcggag aacaggttac
    24781 caacgcgagc atcaacgccc agtgctttag ccgcgtctac cgcgttacgc accatgtcaa
    24841 agtcagcgat agcggcaaag tcatgatctt taaaacggat gcggttaact ttggaatcgg
    24901 tgcaggcacc cataccgata acgacgtcgc gcagttttac gtgcggcaga actgcgccac
    24961 aggaacccac gcggataatt ttcttcacgc cgaaatcggt gatcagttct ttggtgtaga
    25021 tggagcagga cgggataccc ataccgtgac ccattacgga aattttgcgg cctttgtaag
    25081 taccggtgaa gcccagcata ccgcgaacgt tgttcacttc acgggcatct tcaaggaaag
    25141 tttcagcaat atacttcgca cgcagcgggt cgcctggcat caaaactacg tcagcgaaat
    25201 cgcccatttc tgcattaatg tgtggggtag ccattgtttt atccttatca acatgttttc
    25261 accctctccc tgagggagag ggccggggtg agggcatcaa gccgcaccca ttatcagaac
    25321 atggctttgc catattccat atcagaagta ccaaaatatt ttgccagagt ctgaccaata
    25381 tccgcgaagg tttcacggtg acccagtgag cccggtttta ctttcgggcc gtaaaccagt
    25441 accggaatgt gttcacgcgt gtggtcagta ccggtccagg tcggatcaca accgtggtca
    25501 gcagtgagga tcaggatgtc gtcatcgcgc agcagagaca tcagctccgg cagacggcgg
    25561 tcgaacagct ccagacccgc agcataaccg gcgacgtcgc gacggtggcc ccaggaagag
    25621 tcgaagtcaa cgaagttggt gaagacgatg gtgttatcac ccgcttcttt catctctttg
    25681 atggtggcgt caaacagcgc gtccaggcca gttgctttca ctttcttggt gataccgcag
    25741 ttggcgtaga tgtctgcaat tttaccgaca gaaaccacct gaccgtgttt ttcatcaacc
    25801 agtttctgca gcacggtcgg tgctggcggc tcaacagcca ggtcgtgacg gttaccggta
    25861 cgctggaagt taccggcttt gtcgccaata aacggacgag cgataacacg accgatgttg
    25921 tagccgccgt tggtcagctc ttcacgggcg atttcgcaca gttcgtagag tttatccaag
    25981 ccaaaggttt cttcgtggca ggcaatctgg aacacggagt cagcggaggt atagaaaatc
    26041 ggcttgccgg ttttcatgtg ctcttcgccc agctgatcca gaatgaccgt accggaagag
    26101 tggcagttac cgagataacc aggcaggtta gcgcgttcaa ccagtttatc cagcagctct
    26161 tgcgggaagc tgttttcgtg atcggagaag tatccccact caaacagaac cgggacaccg
    26221 gcgatttccc agtgaccaga cggggtatct ttaccggatg acatttcgtg cgcccatgcg
    26281 tacgcgccga taacttcagc gttgccgtcc attcctgccg gaatgaaacc ggtagaacct
    26341 tcatgtgctt tcgccagccc cagacgggtc agatttggca gattgagcgg gcctttacga
    26401 ccgttatcag cttcgccttt ggcacaggct tctgcgatat gacccagggt gtcagccccg
    26461 acgtcaccaa agcgttctgc atcttctgta gcgccgatgc cgaatgagtc cagcaccata
    26521 ataaatgcac gtttcatatg ttctccgtac tcagtgcttc aaatttaaat gatcagatca
    26581 gtataccgtt attcgctgat acggcgatag acagttggtg tgctttccgg tgctttatcg
    26641 gcaagtttaa ttgccgcttt caccgctttc gccgcgtcct gccagctgtt ttcgtctttc
    26701 gcgtggataa cagccagcgg acgctgtccg tctacctggt cgcccagacg cgccatatca
    26761 gtaaagccga cgctgtaatc gatggtgtca gatgcctgac ggcgtccgcc gcccattgca
    26821 accactgcca tccccagcgc gcgggtatcc atttcactga caaaaccttc ggtatcagca
    26881 tagactgctt tcgtcagcat cgctgtcggc agatacttcg cgtagttctc aacgaagtcg
    26941 gtcgggcctt tttgtgccgc taccatacga ccaaagactt ctgccgcttt accgttgtcc
    27001 agcaccgcct gcaatttcgc gcgcgcttcg gcgtcatctt tcgccagttt gccggagatc
    27061 agcatctcca cgcacagcgc catcgtgaca tcaaacagac gcgggttacg atattcaccc
    27121 gtcaggaact gcaccgcttc acgaacttca accgcgttac ctgcactgga ggccagtacc
    27181 tgattcatgt cggtgagcag cgcggtggtg cgcacgccag cgccgttagc cacgccaaca
    27241 atcgcttcgg caagggcttc agagagttcg taggtcggca taaacgcgcc gctacccact
    27301 ttcacgtcca tcaccagcgc atccagacct tccgccagtt tcttcgccag gatcgaggca
    27361 gtgatcagcg ggatggagtc cacggttgcg gtaatatcac gggtcgcgta gaaacgttta
    27421 tccgccggag ccagtgagct ggtctggccg ataatcgcta cgccgacgtc tttaataatt
    27481 tcgcggaaac ggttgtcatc cgggaaaatg tcgaagccag ggatggattc cagtttgtcg
    27541 agcgtaccgc cagtatgacc gaggccgcga ccggagatca tcggaatata gccgccgcag
    27601 gctgcgacca tcggccccaa catcagcgaa gtcacatcgc cgacgccgcc ggtggagtgt
    27661 ttatcaacaa tcgggccatt cagatgcagg cttttccagt cgagaacggt tcctgaatct
    27721 cgcatcgcca tggtcagcga gacacgctca ggcattgtca tatcgtggaa gaaaatggtc
    27781 atcgcgaggg cggcaatctg cccttcggag atagtgttgt cgcgaatacc gttgataaag
    27841 aaacgaattt cttcatcgct tagcgcatga ccatcacgtt ttttacgaat aatttcttgt
    27901 gcgagaaaca aggtaacctc ccgaggaaaa gagtatgagg cggcttttgc cggatgcgtc
    27961 tacgccttat ccggcctacg ggtagatacg tctcgtaggc ctgataagcg cagcgcatta
    28021 ggcgtaaagc atcttactta gtagctgctg gcgctcttac cgtcaccgtg gcccagcgct
    28081 ttcaacaggc ttgccagcag gctggaagca ccaaagcggt agtgacgcgc atctgcccag
    28141 tcagcaccga acagttcatc tgcgatggcg agatatttct gcgcatcttc cgcagtacgc
    28201 acgccgcccg ctggtttgaa accaacggtt ttttctacgc ccatatcacg gatcacttcc
    28261 atcatgatgc gcgcgctttc cggcgtcgcg ttcacagcca ctttaccggt agaggttttg
    28321 atgaagtccg cacccgcttt gatggagatt tcagacgctt tacggatcag cgcttcgtct
    28381 ttcagttcgc cggtttcgat gatcactttc agcagtacat tcgctgccgc gcaagcctct
    28441 ttacaggctt tcaccaggtc aaaaccaacc tgctcgttac ccgccatcag cgcgcggtac
    28501 gggaacacaa cgtcaacttc atcagcaccg taggcgattg ccgcacgggt ttctgccagc
    28561 gcgatttcga tgtcatcgtt accgtgtggg aagttggtta ccgtagcgat tcggatttcc
    28621 ggggtgccct gctctttcag cgttttgcga gcaatcggga taaagcgagg atagatacag
    28681 atagcggcgg tattgccgac cggagttttg gcctgatgac acagagcaat tactttctcg
    28741 tcggtgtcgt cgtcattcag ggtggtcagg tccatcaatt tcagtgcacg caggctgctt
    28801 gctttcagat cagtcatttc attctcctgg cttgtcgcca ataaaattca ccttgcgagt
    28861 ttgttagtat tctaacatct actccgcaac acacttcgat atacatcaca attaaggaaa
    28921 tctacttaca agtttgcatc actgtaatgc gatctggttc aaataattca ctttcaaatg
    28981 aatgcgtcag tggtggcaaa cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa
    29041 tggcgcatca ccttcggcat aaaaggaaaa gaagatgccc acttctcatg aaaatgcact
    29101 gcaacaacgt tgccagcaaa ttgtcaccag cccagtgctt agcccggagc agaagcgcca
    29161 ttttctggct ctggaagcag aaaacaatct gccttatccg cagcttcctg ccgaagcccg
    29221 ccgcgcgctg gatgagggtg taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc
    29281 gcgctatgtc ttacccgatt acgcccgttt tctggcgaac ggttccgaat ggctggagct
    29341 ggaaggcgcg aaagatcttg atgacgcact ctctctgctg accattcttt accaccacgt
    29401 accgtcggtc acatcgatgc cggtctacct ggggcaactg gatgcgttgt tgcaaccgta
    29461 tgttagaatt ctaacacaag acgagatcga tgttcgaata aaacgtttct ggcgttacct
    29521 cgacagaacc ctgccagacg cctttatgca cgccaatatc ggcccgtctg attcgcccat
    29581 tacccgtgca atcttacgtg cagacgcaga gttgaagcag gtttcaccga acctgacctt
    29641 tatctacgat cctgaaatca cccctgatga cctgctgctg gaagtggcga agaacatctg
    29701 tgaatgtagc aaaccgcaca tcgccaacgg tccggtgcat gataaaattt tcacaaaagg
    29761 gggctacggg attgtgagct gttacaactc actgccgctg gcgggtggtg gcagcacgct
    29821 ggtacgcctt aacctgaaag ccattgccga gcgcagcgaa tcgctggatg acttctttac
    29881 gcgcactcta ccgcactact gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt
    29941 cctctatcaa caatcacact tctttgagaa tagcttcctg gtgaaagaag ggctgattaa
    30001 ccctgaacgt tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg ttaacttgct
    30061 gtgtgagaaa gaagggattg ccgcacgtta cggtaaagaa gccgccgcaa atgaagtggg
    30121 ttatcgcatc agcgcgcaac tggcggagtt tgtcgccaat acccccgtga aatatggatg
    30181 gcaaaaacgc gccatgttac acgcacagtc ggggatcagt tccgatattg gcaccacgcc
    30241 gggcgcgcgt ttgccgtatg gcgatgagcc agatccgatc acccatctgc aaactgtcgc
    30301 cccgcatcat gcttattatt attccggcat cagcgacatt ctgacgctcg acgaaaccat
    30361 caaacgtaac ccgcaggcac tggtacagct ttgcctcggt gcctttaaag ccggaatgcg
    30421 tgaatttacc gccaatgtca gcggtaacga tctggttcgc gttaccggtt atatggtacg
    30481 tttgtcggac ttagaaaaat atcgcgccga aggttcacgc accaacacca cctggctggg
    30541 cgaagaagcc gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga taagccatga
    30601 acagcagatg cgctttagtc agtaagatta tccccttctc ctgcgttgac gggccaggca
    30661 gtcgtctggc tctgtttttg cagggctgca atctgcgctg caaaaactgt cacaatccgt
    30721 ggacgatggg acgttgcaat gactgtggag agtgcgtgcc acagtgtccg catcaggcgt
    30781 tgcagattgt tgacggcaaa gtgctgtgga acgctgtggt ctgcgagcag tgtgatacct
    30841 gcctgaagat gtgtccgcaa catgccacgc caatggcgca atccatgagc gtggacgaag
    30901 tgcttagcca tgtccgcaaa gcggtgctgt ttattgaagg gataacggta agcggcggcg
    30961 aagccacgac ccaactgccg tttgtagtgg cgctgtttac tgctatcaaa aacgatccgc
    31021 aactgcgcca cctcacctgc ctggtggaca gtaacggcat gttgagcgaa accggctggg
    31081 aaaaattact cccggtgtgt gacggcgcaa tgctcgatct caaagcgtgg gggagcgaat
    31141 gtcatcaaca cttaaccgga cgcgataatc agcagattaa gcgcagcatc tgtttgcttg
    31201 cagagcgcgg caagctggcg gaactgcgtt tgctggtaat tccagaccag gtggattatt
    31261 tgcatcacat cgatgagctg gcgacgttta tcaagagact tggcgatgtt ccggttcgcc
    31321 tgaatgcgtt tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg agcgccacgc
    31381 cggaagacgt tgagccgttg gctgatgcgt taaaggtgcg cggggtgagc cggttgatat
    31441 ttccggcgct ctatttgtga catgccggat acggcctacg gttcggcaca gacttgtagg
    31501 catgataaga cgcgacaagc gtcgcatcag gcattatgcg ccgactgccg gatgcggcgt
    31561 gaacgcctta tccggcctac ggttcggcac aaacctgtag gcatgataag acgcgtcaag
    31621 cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt atccggccta
    31681 cggttcggca caaacctgta ggcatgataa gacgcgttaa gcgtcgcatc aggcattatg
    31741 cgccgactgc cggatgctgc ctacgaaggg taaactaccc actaacgcta aacaacgcat
    31801 acgtattatt aagcaacact tcggcaatct catcttctgg ttccgggcgc aactcgcaaa
    31861 gcacgtcaaa cacccgcaca gcctgttccg gtcggttagg ctgcccctga aagccgttga
    31921 gcggcatatc cggcgcgtcg gtttccagca acaacgatgc cagcggtaat tttgcgatga
    31981 catcgcgggt tttactggcg cgtggatagg tgatagtacc gcctacgccg attttgtagc
    32041 ccagctgtac aaaccgctcg gcctgttgca ggctgccgga aaaaccgtga accacgccag
    32101 tgcacggtaa atcgtgacgt ttaagatgca tcgccagttt gtcgtgtgta cgccgggaat
    32161 gaaggatcac cggcagatcg tagcgtttcg ccagtttcag ctgttcgtcg agtaaccact
    32221 gctgcctctc aaattgcgga tcgtcgccga agagatccag accgatctcc cccaccgcca
    32281 ccaccttcgc cggacgcctt tccagcgcct gctgtagctg atcaagagac acatcgctat
    32341 gtttttccaa catccccgga tgcaacccca atgcggcata cagcggttga tatttttccg
    32401 ctaacgcctg cacgcgggca aaattttccg cctctgtcgc cggaacaatg atcttgccta
    32461 cgcccgcttg tgccgcgcgt tgcaggctgg cctcttcatc gccactaaac ggcgggaaat
    32521 caaaatggca gtgggtatcg ataaaacggc aaatcaagcc agatcctcat tgttaaatgt
    32581 cgggtcgttc gcctgcggag catcgctcac ttcggcaacc agcgtatcgt tggcgactgg
    32641 cgcaggcgga ataacgatcg attccggcgt caccactggc accagatggc gggtaagcgg
    32701 cgctttttca gttaatagct tgccaaccgt ggcgaggaaa taacgaccgc ataaacgccc
    32761 aagtttatag tcttcacgca gcgccggaat ccgactgcca agcgcgatac tatgtaatgg
    32821 tttcggtgga taaatttcga atatccgcag cttgcccggt ggtttctcaa taaattgctg
    32881 aatgtcacga tagctggttt catgatgctg caccagattg accagcggct gcaggctact
    32941 gtcacccagc cagcgttcca tgcgtttgaa ccactgcggc gtgtagtaca tttgtgacgg
    33001 cacagtgcgg atgacgacca acgttttagc gccctgcctt gccgcctctt taaccggaat
    33061 cgcatcactg atcccgccat ccaagtagtt aatgccttcc agcgacactc cgctacgata
    33121 aaagccaggt atcgcactgg aggcgcgaat cacatccagc cagttttgtt tggttggtaa
    33181 aaagtaatta ggcgcgtagt catcctgacg acaggcgcac atataaaacg atttgccgct
    33241 gtcaaacaac cgcgcggcgg tatccatttg caacggcatc tggcttgcgg tggcctccac
    33301 cagccagtcg agatcgataa gatttcctcc acggacaaag cgcaatggat cgaaaaattc
    33361 gcgttttgtg gtatagcgca tgatgacttt gcgcgcgtaa ccgggctgat tgcagataaa
    33421 cgccgagagg ttctgcgccc cggcagatgt gccgagataa agatcgaaag gattaaactg
    33481 cgcgcgcata aactcatcca gcacgccagc cgtgaaaatt ccacgctgtc cgccgccttc
    33541 gcacaccaga gctattcgtc caggctggaa cggccttagc gacaacggcg caatattacc
    33601 aagcgttaca ggtattcgct gccccacctc tgccttcctg tttttgttgt gaaagaatga
    33661 caaagtaacg caatttatta ttcgctaaaa ccgcaaaagc cagtccgctg gactggcttt
    33721 ggataaaatt ttcttaatgg tctgaaatct agggtcgttt tcggcccatg aacaaactca
    33781 ccaggaacag aataatcccg acgacaaaga caattttagc tgcgcctgca gcggtaccgg
    33841 ccagaccacc aaacccaagt gcggcggcga ttaacgcgat aaccagaaat atgatgcccc
    33901 aacgaaacat aagcgtctcc tttaccatag ttaatgtcac cgcttgatat gagcgtgaaa
    33961 atcactcaat gcgatatttt tagtgtggtg cacatcgcgc ctcccgacaa agttcgggag
    34021 gacgaattac gacgaattac ttagttttca gatcattttt aacgcttttc acaccatcta
    34081 ccgctttggc gatactttca gcacggtcgc tctgtgcctg ggaatcgacg gtaccggaga
    34141 gctgaaccac gccgtcggtg gtttcaactt tcacatgacg ggaagggacg atatcgtccg
    34201 ccagcagttt ggctttgatt tcactggtgg tggcggtgtc acccgcgtag cccttcacag
    34261 agccttcttt agcgtcgcga acgtgcagtt tgtcgctgac agaggtcacc ccttcaacgc
    34321 ctttcgccac tttcactgcc tcttcggcct gggcctggct ttcaacgaaa ccgctcaggg
    34381 tcacgacttt ttgatcggtt tttacagaga tatcggtgct cttgatgttg tcatgatcca
    34441 ccagggccgc cttcactttc gcggtgatgg cgctgtcatc catgaaatta ccgactttat
    34501 tcatagagct atcgactttt tgccctgcgc tttcattggt agtctgtgcg ttgttttccg
    34561 cgtaggcaga gccggtcgcg acggcagagg tcaacattac agccagcaga gttttcgaaa
    34621 tcttcagtct tgtcatagtc atcgatttat tcctgtatgt ttgctcgtaa tttgagcctg
    34681 gcaacacgag gttgcattgc tgaataggga gagacttcac cctctacaga agtcaatggt
    34741 cgccatcaca aaagcgatgg gtgatgaata acgaccatta cagcctctga atcagttatt
    34801 aatatcggta gaatgacaat cgacggcttt agatactgat atctacgcat tgaacggtat
    34861 ttaatgccgt cagaaatgtc attactttgt taaatataga tcacaatttt gaaaccgctc
    34921 gggatatcag cgaaaacata agcaaaagtg aatgttttaa gaacattccg taagcggcta
    34981 ataaggaagg gaaattgaca gggcgaggcg gttgccgcgc cctggaggca agaattaatg
    35041 ctcgcgggtc tggtggaact gaacgtccgg ataacgttcc tgtgccaggc gcaggttaac
    35101 catgctggta gcgatgtaag cgagattatc gccgccatca agcgccagtt ggctttcgtt
    35161 cttacgcttg aactcttcaa atttcttcgc gtccgcacat tctacccacc gggcggtggc
    35221 aacgttgact gattcatata ctgcttcaac gttgtattcg ctcttcaggc gcgataccac
    35281 tacatcaaac tgcagcacac caaccgcacc aacgatcagg tcgttgttgg agatcggacg
    35341 gaacacctgc accgcgccct cttcggaaag ctgtaccagc cctttgagca gctgtttttg
    35401 cttcagcgga tctttcaggc ggatacgacg gaacagttct ggtgcgaagt tcggaatacc
    35461 ggtgaacttc atcatctcac cctgggtaaa ggtgtcgccg atctgaatgg tgccgtggtt
    35521 gtgcaggccg aggatatcgc caggatacgc ttcttcaacg tgcgaacggt cacccgccat
    35581 aaaggtcagc gcgtcagaga tcaccacgtc tttcgcggtg cgcacctggc gcagcttcat
    35641 gcctttttca tatttaccgg ataccacgcg cataaacgcc acgcggtcgc ggtgtttcgg
    35701 gtccatgttg gcctgaattt taaatacgaa gccggtaaat ttgtcttcgc tcgcttgtac
    35761 ggtacgggta tcagtctgac gcggcatcgg cgcaggtgcc cactccacca ggccatccaa
    35821 catatgatca acgccgaagt tacccagcgc agtaccgaag aataccggag tgatttcgcc
    35881 cgcaaggaac agctctttgt cgaactcgtt agacgcgcct ttaaccagtt ccagttcgtc
    35941 acgcagctgc tgtgccagat cttcaccaac cgcagcatcg agatccgggt tattcagccc
    36001 tttaacaatg cggacttcct gaatggtgtg ccctttaccg ctctgataga gataggtttc
    36061 atctttataa aggtggtaaa cgcctttaaa cagcttgccg cagccaattg gccaggtgat
    36121 cggtgcgcag ccaattttca gctcgttctc aacttcatcg agcaattcca tcgggtcgcg
    36181 gatatcacgg tcaagtttgt tcataaaggt gaggatcggc gtgtcgcgca gacgggtaac
    36241 ttccatcagc ttacgggtac gatcttcaac accttttgcg gcgtcgataa ccatcaggca
    36301 gcagtccacc gccgtcaggg tacgataggt atcttccgag aagtcttcat gccccggggt
    36361 gtcgagcagg ttaaccaggc aatcgtgata cggaaactgc atcacagacg tagtaatgga
    36421 gatcccacgc tgcttttcca tctccatcca gtccgactta gcgtgctggt tggaaccacg
    36481 gccttttact gtaccggcgg tctgaatggc ctgtccgaac agcagcacct tctcggtgat
    36541 ggtagtctta ccggcgtccg ggtgagaaat aattgcaaag gttcttcttt tcgctacctc
    36601 tgcggccaaa aggcttgtca taattgttgt ctctttgatt atgtacaatc atatgtacaa
    36661 agaaaaattc atgccgctga ttgtacattt tggaggtatc agagcatagc agaacatgat
    36721 cagtaggtgt atttgtagcc aaagaacatg tgtatatgca gcaatctttg ctctattgac
    36781 actgtttatt catacagtta aaaataacac tgtatacaaa cacagtgaca ggaggtgtta
    36841 tgcgtattga gatctgtatc gctaaagaaa aaatctgcaa aatgccaaaa ggctctgttc
    36901 ctgccctgca acaggaaatg ctacgacgtg tcggtaagcg ttatgatgat gtagatgtga
    36961 ttgtgaaatc tgccagtaat gatggactca caattttaag agccgtggat aaagaatccg
    37021 cgcaagaatt tgtacaggaa acgctgcagg aagtctggga aacagcggat gactggttta
    37081 tccggtgaca tcagcacggg gagctggcag ctcccctctt ttattgtgtt atcagtgcag
    37141 caagtacaca gagtgttacc acaccaccca gccatacaaa cagcatgaag cacatcgcac
    37201 ccgctgtgta ttttcgcttt ccgcatctga ttttttcctg ctccattctc actgccaccg
    37261 ttcaactctc attacccgac ttcacgaata tgcattccgg ctacgcacgt ccaaccaggt
    37321 gcttcgcact cctgtactta agaccgtttt taccaagtcc catatacaga aaggtgttca
    37381 ccgtcttcgt gtttat
//
LOCUS       DAZGAN020000039        36653 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGAN020000039 DAZGAN020000000
VERSION     DAZGAN020000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 36653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.039"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            173..3889
                     /gene="yfaL"
                     /locus_tag="ACHKYO_RS10860"
                     /old_locus_tag="pgaptmp_002172"
     CDS             173..3889
                     /gene="yfaL"
                     /locus_tag="ACHKYO_RS10860"
                     /old_locus_tag="pgaptmp_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HGU4429023.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTLTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGNG
                     QTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVGGYSDNQ
                     GESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEQDDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVSQSQGDD
                     IQMRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAVKQRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
     gene            complement(4017..4739)
                     /gene="ubiG"
                     /locus_tag="ACHKYO_RS10865"
                     /old_locus_tag="pgaptmp_002173"
     CDS             complement(4017..4739)
                     /gene="ubiG"
                     /locus_tag="ACHKYO_RS10865"
                     /old_locus_tag="pgaptmp_002173"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HGU4429024.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            4886..7513
                     /gene="gyrA"
                     /locus_tag="ACHKYO_RS10870"
                     /old_locus_tag="pgaptmp_002174"
     CDS             4886..7513
                     /gene="gyrA"
                     /locus_tag="ACHKYO_RS10870"
                     /old_locus_tag="pgaptmp_002174"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HGU4429025.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7662..9349
                     /gene="yfaA"
                     /locus_tag="ACHKYO_RS10875"
                     /old_locus_tag="pgaptmp_002175"
                     /pseudo
     CDS             7662..9349
                     /gene="yfaA"
                     /locus_tag="ACHKYO_RS10875"
                     /old_locus_tag="pgaptmp_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            9346..9969
                     /gene="yfaT"
                     /locus_tag="ACHKYO_RS10880"
                     /old_locus_tag="pgaptmp_002176"
     CDS             9346..9969
                     /gene="yfaT"
                     /locus_tag="ACHKYO_RS10880"
                     /old_locus_tag="pgaptmp_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HGU4429026.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9993..14507
                     /locus_tag="ACHKYO_RS10885"
                     /old_locus_tag="pgaptmp_002177"
     CDS             9993..14507
                     /locus_tag="ACHKYO_RS10885"
                     /old_locus_tag="pgaptmp_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HGU4429027.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLGDA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14508..15970
                     /locus_tag="ACHKYO_RS10890"
                     /old_locus_tag="pgaptmp_002178"
                     /pseudo
     CDS             14508..15970
                     /locus_tag="ACHKYO_RS10890"
                     /old_locus_tag="pgaptmp_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            15975..16751
                     /gene="yfaP"
                     /locus_tag="ACHKYO_RS10895"
                     /old_locus_tag="pgaptmp_002179"
     CDS             15975..16751
                     /gene="yfaP"
                     /locus_tag="ACHKYO_RS10895"
                     /old_locus_tag="pgaptmp_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HGU4429028.1"
                     /translation="MRKIFLPLLLVALSPVVHSEGVQEVEIDAPLSGWHPVEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(16825..18009)
                     /gene="atoB"
                     /locus_tag="ACHKYO_RS10900"
                     /old_locus_tag="pgaptmp_002180"
     CDS             complement(16825..18009)
                     /gene="atoB"
                     /locus_tag="ACHKYO_RS10900"
                     /old_locus_tag="pgaptmp_002180"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HGU4429029.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKIFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18040..19362)
                     /gene="atoE"
                     /locus_tag="ACHKYO_RS10905"
                     /old_locus_tag="pgaptmp_002181"
     CDS             complement(18040..19362)
                     /gene="atoE"
                     /locus_tag="ACHKYO_RS10905"
                     /old_locus_tag="pgaptmp_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HGU4429030.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19359..20009)
                     /gene="atoA"
                     /locus_tag="ACHKYO_RS10910"
                     /old_locus_tag="pgaptmp_002182"
     CDS             complement(19359..20009)
                     /gene="atoA"
                     /locus_tag="ACHKYO_RS10910"
                     /old_locus_tag="pgaptmp_002182"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HGU4429031.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20009..20671)
                     /gene="atoD"
                     /locus_tag="ACHKYO_RS10915"
                     /old_locus_tag="pgaptmp_002183"
     CDS             complement(20009..20671)
                     /gene="atoD"
                     /locus_tag="ACHKYO_RS10915"
                     /old_locus_tag="pgaptmp_002183"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HGU4429032.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(20867..22252)
                     /gene="atoC"
                     /locus_tag="ACHKYO_RS10920"
                     /old_locus_tag="pgaptmp_002184"
     CDS             complement(20867..22252)
                     /gene="atoC"
                     /locus_tag="ACHKYO_RS10920"
                     /old_locus_tag="pgaptmp_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HGU4429033.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22249..24075)
                     /gene="atoS"
                     /locus_tag="ACHKYO_RS10925"
                     /old_locus_tag="pgaptmp_002185"
     CDS             complement(22249..24075)
                     /gene="atoS"
                     /locus_tag="ACHKYO_RS10925"
                     /old_locus_tag="pgaptmp_002185"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HGU4429034.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24242..27091
                     /gene="rcsC"
                     /locus_tag="ACHKYO_RS10930"
                     /old_locus_tag="pgaptmp_002186"
     CDS             24242..27091
                     /gene="rcsC"
                     /locus_tag="ACHKYO_RS10930"
                     /old_locus_tag="pgaptmp_002186"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HGU4429035.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27291..27941)
                     /gene="rcsB"
                     /locus_tag="ACHKYO_RS10935"
                     /old_locus_tag="pgaptmp_002187"
     CDS             complement(27291..27941)
                     /gene="rcsB"
                     /locus_tag="ACHKYO_RS10935"
                     /old_locus_tag="pgaptmp_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HGU4429036.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(27986..29275)
                     /locus_tag="ACHKYO_RS10940"
                     /old_locus_tag="pgaptmp_002188"
     CDS             complement(27986..29275)
                     /locus_tag="ACHKYO_RS10940"
                     /old_locus_tag="pgaptmp_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4429037.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKA"
     gene            complement(29309..29563)
                     /gene="xisR"
                     /locus_tag="ACHKYO_RS10945"
                     /old_locus_tag="pgaptmp_002189"
     CDS             complement(29309..29563)
                     /gene="xisR"
                     /locus_tag="ACHKYO_RS10945"
                     /old_locus_tag="pgaptmp_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4429038.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(29571..29717)
                     /locus_tag="ACHKYO_RS10950"
                     /old_locus_tag="pgaptmp_002190"
     CDS             complement(29571..29717)
                     /locus_tag="ACHKYO_RS10950"
                     /old_locus_tag="pgaptmp_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429039.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            complement(29973..30875)
                     /locus_tag="ACHKYO_RS10955"
                     /old_locus_tag="pgaptmp_002191"
     CDS             complement(29973..30875)
                     /locus_tag="ACHKYO_RS10955"
                     /old_locus_tag="pgaptmp_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429040.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQEA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLMEATKFAYEGSVNG"
     gene            complement(30872..31100)
                     /locus_tag="ACHKYO_RS10960"
                     /old_locus_tag="pgaptmp_002192"
                     /pseudo
     CDS             complement(30872..31100)
                     /locus_tag="ACHKYO_RS10960"
                     /old_locus_tag="pgaptmp_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(31117..31479)
                     /locus_tag="ACHKYO_RS10965"
                     /old_locus_tag="pgaptmp_002193"
     CDS             complement(31117..31479)
                     /locus_tag="ACHKYO_RS10965"
                     /old_locus_tag="pgaptmp_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HGU4429041.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYEDLPYEEAQDEYTGETNIMATIQIGDLACTDEDMLGEAWLA
                     GMLIAAEIISIEPAGTLS"
     gene            complement(31472..32338)
                     /locus_tag="ACHKYO_RS10970"
                     /old_locus_tag="pgaptmp_002194"
     CDS             complement(31472..32338)
                     /locus_tag="ACHKYO_RS10970"
                     /old_locus_tag="pgaptmp_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGU4429042.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVITTEQLAAGYGATPIRIRQ
                     NHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASNVRSLILWTERGAANHAK
                     LLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMALENKTLS
                     ATVDSLEKHFSKGMTVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTAS
                     YARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKH
                     DKAIYTPEAEHE"
     gene            complement(32362..32946)
                     /locus_tag="ACHKYO_RS10975"
                     /old_locus_tag="pgaptmp_002195"
     CDS             complement(32362..32946)
                     /locus_tag="ACHKYO_RS10975"
                     /old_locus_tag="pgaptmp_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429043.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGRTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            complement(32933..33493)
                     /locus_tag="ACHKYO_RS10980"
                     /old_locus_tag="pgaptmp_002196"
     CDS             complement(32933..33493)
                     /locus_tag="ACHKYO_RS10980"
                     /old_locus_tag="pgaptmp_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429044.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DTEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQRLMHPEYGEVFHPGEITPEYYRAHGRCFHRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDSN"
     gene            complement(33901..34263)
                     /locus_tag="ACHKYO_RS10985"
                     /old_locus_tag="pgaptmp_002197"
     CDS             complement(33901..34263)
                     /locus_tag="ACHKYO_RS10985"
                     /old_locus_tag="pgaptmp_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429045.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGRKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITGCVNH"
     gene            complement(34438..34638)
                     /locus_tag="ACHKYO_RS10990"
                     /old_locus_tag="pgaptmp_002198"
     CDS             complement(34438..34638)
                     /locus_tag="ACHKYO_RS10990"
                     /old_locus_tag="pgaptmp_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429046.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCLIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            complement(34635..34826)
                     /locus_tag="ACHKYO_RS10995"
                     /old_locus_tag="pgaptmp_002199"
     CDS             complement(34635..34826)
                     /locus_tag="ACHKYO_RS10995"
                     /old_locus_tag="pgaptmp_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429047.1"
                     /translation="MKMFKGLTNEPKTAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(34828..35205)
                     /locus_tag="ACHKYO_RS11000"
                     /old_locus_tag="pgaptmp_002200"
     CDS             complement(34828..35205)
                     /locus_tag="ACHKYO_RS11000"
                     /old_locus_tag="pgaptmp_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4429048.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCDPMWLLTSFHSDTTKES"
     gene            complement(35291..35950)
                     /locus_tag="ACHKYO_RS11005"
                     /old_locus_tag="pgaptmp_002201"
     CDS             complement(35291..35950)
                     /locus_tag="ACHKYO_RS11005"
                     /old_locus_tag="pgaptmp_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU4429049.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
     gene            36290..36583
                     /locus_tag="ACHKYO_RS11010"
                     /old_locus_tag="pgaptmp_002202"
     CDS             36290..36583
                     /locus_tag="ACHKYO_RS11010"
                     /old_locus_tag="pgaptmp_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429050.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
BASE COUNT         8841 a         8913 c         9537 g         9362 t
ORIGIN      
        1 taactggtat cccttaaggt tattaacttg aattatgacg cactgatact attcatcaaa
       61 taataacata atagccattg catcgggttg gacaatttac aaaagaaaga taacccatat
      121 tgtataatgc gtttaaatat gccgtcttat ataaaatgat ggttagatga atatgcggat
      181 tatctttcta cgcaaggagt atttatctct actcccgtca atgattgcat ctcttttctc
      241 tgctaacggt ttcgcggcgg ccattgattt atgccaggga tatgatatca aagcgagttg
      301 tcacgccagc aggcaaagcc tttcaggcat tacgcaggac tggagtgtcg ccgatgggca
      361 atggctggtt ttttcggata tgaccaataa cgccagcgga ggagccgtat ttttgcaaca
      421 aggagcggaa ttttcactat taccagaaaa tgaaactgga atgactctgt ttgccaataa
      481 caccgttaca ggagaatata ataacggcgg ggccatattt gctaaagaaa actcaacgct
      541 gaatcttact gatgttattt tttctggtaa cgtcgcaggc ggctatggtg gcgcaatcta
      601 ttcttctggt actaatgata ctggtgccgt cgatttacgt gtcactaacg ccatgtttcg
      661 caataatatc gctaatgatg gcaaaggtgg cgcaatttat accattaata atgacgtcta
      721 tttaagtgat gttatttttg ataacaacca ggcatataca ttaacaagtt acagtgatgg
      781 cgatggcggg gcaatcgatg ttaccgataa taatagcgac agcaagcatc cttcaggtta
      841 tacgataata aataacactg cctttacaaa taacactgcc gaaggttatg gcggggcgat
      901 atataccaat agcgcgacgg ctccctatct tattgatatt tctgttgatg acagctacag
      961 ccagaacggc ggcgtgttag tggatgagaa caacagcgca gcaggctatg gagatggtcc
     1021 ttccactgcg gcgggtggct ttatgtatct cggcttaagt gaagttacct ttgatattgc
     1081 cgacggaaaa acgctggtta ttggcaatac agagaatgac ggagctgttg actctattgc
     1141 tggtaccggg ttgatcacca aaacaggttc cggcgatctg gtacttaatg cagataacaa
     1201 tgactttact ggtgagatgc agattgaaaa cggtgaagtt accctgggcc gcagcaactc
     1261 cctgatgaat gtcggcgata cgcattgcca ggacgatccg caagactgct acggtctgac
     1321 gatagggagt attgataagt accagaatca ggcagagcta aacgttggct ccacccaaca
     1381 aacctttgtg cactcattga cgggctttca gaatggcact ttaaatatcg atgctggtgg
     1441 caatgttact gttaatcaag gcagttttgc tggcaccatc gaaggtgctg gtcagctcac
     1501 cattgcgcaa aacggcagct acgtgctgtc gggggcgcag tcgatggcgc taaccggcga
     1561 tatagtcgtt gatgatggtg cggtgctttc gctggaaggc gacgcggcag atctcgccgc
     1621 tctccaggac gatccgcagt cgatcgtgtt aaacggcggt gtgctcgatc tctctgattt
     1681 ctccacctgg cagagcggca catcatacaa tgatggcctt gaagtcagtg gcagcagcgg
     1741 aacggttatc ggcagtcagg atgtggtaga tcttgcaggt ggcgacaatt tgcatatcgg
     1801 cggcgacggg aaagatggcg tctacgtggt ggtcgatgcg agcgacgggc aggtaagtct
     1861 ggcaaacaat aatagttatt tgggcacaac acaaatcgcc tccggtacgc tgatggtgag
     1921 cgacaactcg cagcttggag atacccacta taaccgccag gttatcttta ccgataagca
     1981 acaagaaagc gtgatggaga ttactgccaa tgtcgatact cgctctacga cgactgagca
     2041 tgggcgtgat attgaaatgc gcgccgacgg tgaagtggca gttgatgcgg gggtagacac
     2101 gcagtggggc gcactgatgg ctgacagcag cgggcagcat caggatgagg gtagcacatt
     2161 tactaaaacg ggggcgggta cgctggagct gaccgccagc ggtacaacgc agtcggcagt
     2221 acgtgtcgaa gaaggcaccc tgaaaggcga tgttgcggat atctttcctt atgcttcgtc
     2281 actgtgggtc ggtgatgggg cgacgttcgt tactggcgcg gatcaggata ttcagtctat
     2341 cgatgctact tccagcggca ctatcgatat cagcgatggt acggttttgc gtctgaccgg
     2401 gcaggatact tccgtcgccc ttaatgcctc attgtttaac ggcgatggga cgctggtgaa
     2461 tgccaccgat ggcgtgacgt tgacaggtga gcttaatacc aaccttgaaa ctgacagcct
     2521 gacctatctt tccgacgtga cggttaatgg caatctgacc aatacgtccg gtgcggttag
     2581 cctgcaaaat ggcgtcgctg gcgatacgct gacggtaaac ggtgattata ccggcggcgg
     2641 tacgctactg ctcgatagcg aattaaacgg cgatgactcg gtaagcgatc aattggtgtt
     2701 gaacggtaat actgctggca acacaactgt ggtggttaac tccattacag ggattggtga
     2761 gccgacatcg acaggcatta aagtggttga tttcgcagcc gatcccacgc agtttcaaaa
     2821 caatgcgaag ttcagtctgg caggcagcgg ctacgtcaat atgggagcat atgactacac
     2881 gctggtggaa gataacaacg actggtatct gcgatcgcaa gaagtaacgc caccattgcc
     2941 acctgatcca gaccctgacc caacacccga tccaatcccc gcttaccagc cggtgctgaa
     3001 tgccaaagtt ggcggttatc tcaataatct gcgggcggca aatcaggcgt ttatgatgga
     3061 gcgacgcgat catgcaggtg gcaatggtca gacgctgaat ttacgtgtta tcggcggacg
     3121 ttatcattac acggcggcag ggcaactggc tcagcatgaa gacacttcta cggtgcagct
     3181 tagcggtgac ctgtttagcg ggcgctgggg cgatgatggc gagtggatgc ttggggcggt
     3241 tggtggctac agtgataatc agggcgagag ccgctcgaat atgaccggaa ctcgcgccga
     3301 taaccagaac cacggttatg ccgttgggct gacctcaagc tggtatcagc acggtaatca
     3361 gaagcagggg gcctggctgg atagctggct gcaatacgcg tggtttaaca atgatgtttc
     3421 cgaacaagac gatggcacag atcattacca ctcgtcgggg attatcgcct cgctggaagc
     3481 cgggtatcag tggttaccgg ggcgtggtgt ggtgattgaa ccgcaggcgc aggtgattta
     3541 tcagggcgtg cagcaggatg attttaccgc cgctaaccgt gcgcgcgtgt cacaatcgca
     3601 gggtgatgat attcagatgc ggctgggttt acacagcgaa tggcgtaccg ctgttcatgt
     3661 cataccaaca ttagatctga attattatca cgatccccat tcgacggaaa ttgaagagga
     3721 tggcagcact atcagtgacg atgcggtgaa gcaacggggt gaaataaaag tgggagtcac
     3781 gggcaatatc agtcagcgag tgtccctgcg cggcagcgtg gcgtggcaga aagggagtga
     3841 tgattttgcc cagatggcag ggtttttgtc gatgacggtg aaatggtaat cactgtctag
     3901 tcggcacggt tgccggatgc ggcgtaaacg ccttatccgg cctacgggga gcatttgtag
     3961 gcctgataag acgcggcaag cgtcgcatca ggcattggct cggccaaaca acaacctcac
     4021 ttattctgcg tgtgcagcat atagttcaca tccacgccgg ggccgagttt aaaagtatta
     4081 gtgagcgggt tgtaatgtag cccggttata tgtcgctctt taaggcttgt ctgatctacc
     4141 cagccaagca gttctgccgg tttaataaac ttcttcacat catgcgtgcc tttgggcacc
     4201 atgcgcaaaa tatattccgc gccaaccacc gccatcagcc atgacttgcc gttgcggtta
     4261 agcgtcgaga aaaagacatc gccgcctggt ttcaccagtt gcgcacaggc tctgaccact
     4321 gactgcggat cggggacgtg ctccagcatc tccatgcagg tcaccacatc atactgcccg
     4381 gcatgttttg ccgcgtgctc ttccacggtt tcctgcacat aatccacctg aatgccgctt
     4441 tccagcgcgt gcagttttgc cacctgcaat ggctcaaagc ccatatccag accggtcacc
     4501 gtcgcgcctt cgcgcgccat actctcggcc agaatgccgc cgccacaacc gacatcgagc
     4561 acctttttgc caaataaacc gccagcacgc tcggcaatat agcccagacg cagcgggtta
     4621 atgcggtgca acggtttgaa ctcaccttca agatcccacc agcgggaggc gacggcttca
     4681 aatttagcga tctcttcgtg gtctacgtta tggttttccg gcgatttttc ggcattcatt
     4741 ggcacttcta ctccgtaatt ggcaagacaa acgagtatat caggcattgg atgtgaataa
     4801 agcgtatagg tttacctcaa actgcgcggc tgtgttataa tttgcgacct ttgaatccgg
     4861 gatacagtag agggatagcg gttagatgag cgaccttgcg agagaaatta caccggtcaa
     4921 cattgaggaa gagctgaaga gctcctatct ggattatgcg atgtcggtca ttgttggccg
     4981 tgcgctgcca gatgtccgag atggcctgaa gccggtacac cgtcgcgtac tttacgccat
     5041 gaacgtacta ggcaatgact ggaacaaagc ctataaaaaa tctgcccgtg tcgttggtga
     5101 cgtaatcggt aaataccatc cccatggtga ctcggcggtt tatgacacga tcgtccgtat
     5161 ggcgcagcca ttctcgctgc gttacatgct ggtagacggt cagggtaact tcggttccat
     5221 cgacggcgac tctgcggcgg caatgcgtta tacggaaatc cgtctggcga aaattgccca
     5281 tgaactgatg gccgatctcg aaaaagagac ggtcgatttc gttgataact atgacggcac
     5341 ggaaaaaatt cccgacgtca tgccaaccaa aattcctaac ctgctggtga acggttcttc
     5401 cggtatcgcc gtaggtatgg caaccaacat cccgccgcac aacctgacgg aagtcatcaa
     5461 cggttgtctg gcgtatatcg atgatgaaga catcagcatt gaagggctga tggaacacat
     5521 cccggggccg gacttcccga cggcggcaat cattaacggt cgtcgcggta ttgaagaagc
     5581 ttaccgtacc ggtcgcggca aggtgtatat ccgcgctcgc gcagaagtgg aagttgacgc
     5641 caaaaccggt cgtgaaacca ttatcgtcca cgaaattccg tatcaggtaa acaaagcgcg
     5701 cctgatcgag aagattgcgg aactggtaaa agaaaaacgc gtggaaggca tcagcgcgct
     5761 gcgtgacgag tctgacaaag acggtatgcg catcgtgatt gaagtgaaac gcgatgcggt
     5821 cggtgaagtt gtgctcaaca acctctactc ccagacccag ttgcaggttt ctttcggtat
     5881 caacatggtg gcattgcacc atggtcagcc gaagatcatg aacctgaaag acatcatcgc
     5941 ggcgtttgtt cgtcaccgcc gtgaagtggt gacccgtcgt actattttcg aactgcgtaa
     6001 agctcgcgat cgtgctcata tccttgaagc attagccgtg gcgctggcga acatcgaccc
     6061 gatcatcgaa ctgatccgtc atgcgccgac gcctgcagaa gcgaaaactg cgctggttgc
     6121 taatccgtgg cagctgggca acgttgccgc gatgctcgaa cgtgctggcg acgatgctgc
     6181 gcgtccggaa tggctggagc cagagttcgg cgtgcgtgat ggtctgtact acctgaccga
     6241 acagcaagct caggcgattc tggatctgcg tttgcagaaa ctgaccggcc ttgagcacga
     6301 aaaactgctc gacgaataca aagagctgct ggatcagatc gcggaactgt tgcgtattct
     6361 tggtagcgcc gatcgtttga tggaagtgat ccgtgaagag ctggagctgg ttcgtgaaca
     6421 gttcggtgac aaacgtcgta ctgaaatcac cgccaacagc gcagacatca acctggaaga
     6481 tctgatcact caggaagatg tggtagtgac gctctctcac cagggctacg ttaagtatca
     6541 gccgctttct gaatacgaag cgcagcgtcg tggcgggaaa ggtaaatctg ccgcacgtat
     6601 taaagaagaa gactttatcg accgactgct ggtggcgaac actcacgacc atattctgtg
     6661 cttctccagc cgtggtcgcg tctattcgat gaaagtttat cagttgccgg aagccactcg
     6721 tggcgcgcgc ggtcgtccga tcgtcaacct gctgccgctg gagcaggacg aacgtatcac
     6781 tgcgatcctg ccagtgaccg agtttgaaga aggcgtgaaa gtcttcatgg cgaccgctaa
     6841 cggtaccgtg aagaaaactg tcctcaccga gttcaaccgt ctgcgtaccg ccggtaaagt
     6901 ggcgatcaaa ctggttgacg gcgatgagct gatcggcgtt gacctgacca gcggcgaaga
     6961 cgaagtaatg ctgttctccg ctgaaggtaa agtggtgcgc tttaaagagt cttctgtccg
     7021 tgcgatgggc tgcaacacca ccggtgttcg cggtattcgc ttaggtgaag gcgataaagt
     7081 cgtctctctg atcgtgcctc gtggcgatgg cgcaatcctc accgcaacgc aaaacggtta
     7141 cggtaaacgt accgcagtgg cggaataccc aaccaagtcg cgtgcgacga aaggggttat
     7201 ctccattaag gttactgaac gtaatggttt agtggttggc gcggtacagg tagatgactg
     7261 cgaccagatc atgatgatca ccgatgccgg tacgctggta cgtactcgcg tttcggaaat
     7321 cagcatcgta ggccgtaaca cccagggcgt gatcctcatc cgtactgcgg aagatgaaaa
     7381 cgtagtgggt ctgcaacgtg ttgctgaacc ggttgacgag gaagatctgg ataccatcga
     7441 tggcagtgcc gcggaagggg acgatgaaat cgctccggaa gtggacgttg acgacgagcc
     7501 agaagaagaa taattttact tcttcatgcc aaaagggagc tatctccctt gtttgaattg
     7561 aaaagtccag gctgcaaagt ctgggctttt gtcgtattag ggcacggtaa agtttggctg
     7621 tgcccgtaaa aaatggctgg ctatacacaa ggaatgtggc aatgagtggt gaaaaaaagg
     7681 cgaaaggctg gcggttctat ggtcttgtag gttttggcgc aatagcactg ctttccgctg
     7741 gcgtctgggc gttgcaatat gctggcagtg ggccagaaaa aacgttgtcg ccgctggtgg
     7801 tgcacaacaa tctgcaaatc gatctcaatg agccggacct ctttctcgac agcgactctc
     7861 tgagccagct tcccaaagat ctcctcacca ttccgtttct ccacgatgtt ctgagcgaag
     7921 atttcgtttt ctattatcag aatcatgccg atcgtctggg cattgaaggc agcattcgtc
     7981 gtattgtcta tgaacacgat ctcacgctga aagataagct ctttcgtcac tcttagatca
     8041 gcccgcgcag gcagcgctgt ggcacgataa acaaggccat ctttcacatt atatggtgct
     8101 gatccagcgc agtggtttaa gcaaactgct ggagccattg ttgtttgccg ctaccagcga
     8161 cagccagtta agcaaaacgg aaatcagtag catcaagata aatagtgaaa ctattcctgt
     8221 ttatcagttg cgctataacg gcaataacgc cctgatgttc gcgacttatc aggacaagat
     8281 gctggtgttt tccagcacgg atatgttgtt taaagatgat cagcaggata ccgaagccac
     8341 ggcgatcgca ggtgatttgt tgagcggcaa aaaacgctgg caagccagct tcggtctgga
     8401 agagcgtact gccggaaaaa caccagtacg ccagcgcatc gtggtcagcg ccaggttgct
     8461 gggttttggc taccagcgat taatgccttc ttttgccgga gtgcgtttcg aaatgagcaa
     8521 cgacggctgg catagctttc tggcgttaaa tgatgaatcc gcgagtgtgg atgccagttt
     8581 cgattttacg ccggtctgga acagtatgcc tgccggggcc agcttctgtg tggcggtgcc
     8641 gtattcacac ggtattgccg aagagatgct ttcgcacatc agccaggaaa acgacaagct
     8701 gactggggcg ttagacggtg ccgcggggct gtgctggtat gaagactcaa aattgcaaac
     8761 cccgctgttt gtcggtcagt ttgatggcac tgccgaacaa gcgcaattgc cagggaaact
     8821 gtttacgcaa aatattggtg cgcacgaaag caaagcgcca gaaggtgttt tgccggtaag
     8881 ccagactcag cagggcgaag ggcaaatctg gcgtcgcgaa gtgagttccc gatacggcca
     8941 gtatccgaaa gcgcaggcgg cgcaacccga tcaattaatg tcggattatt ttttccgcgt
     9001 gtcgctggcg atgcaaaaca aaacgctgct tttctccctc gatgacacgc tggttaataa
     9061 cgcgctgcaa acactgaata aaacccgccc ggcaatggtg gatgtaatac ccactgatgg
     9121 catcgttccg ctctatatca atccacaagg cgtggcgaaa ctgctgcgta acgaaacgtt
     9181 gaccagtctg ccgaagaatc tcgaaccggt tttttataac gccgcacaaa ctttattaat
     9241 gccgaagctg gacgctttat ctcaacaacc gcgttatgtc atgaagctgg cccagatgga
     9301 acccggtgcc gcctggcagt ggttacccat aacctggcag ccgctatgag gcacgggctg
     9361 ctggcgctga tttgctggct gtgttgtgtt gttgcccata gcgaaatgct gaatgtcgaa
     9421 caatccgggc tgtttcgcgc ctggtttgta cgcattgcac aagaacagct ccgccagggg
     9481 ccaagtccac gctggtatca gcaggattgt gcgggcctgg tgcgatttgc ggcgaacgag
     9541 acgctgaaag ttcacgatag taaatggcta aaaagtaacg gtttctctag ccagtatttg
     9601 ccgccagaga tgacgctaac acctggacag cgtcaactgg cgcaaaactg gaatcagggg
     9661 aacgggaaaa ccggccccta cgtgaccgcg attaatttga ttcagtacaa cagccagttt
     9721 attggccagg acataaacca ggcgctgcct ggcgatatga tttttttcga tcagggcgat
     9781 gcccagcact taatggtctg gatggggcgt tacgtcatct accacaccgg aagcgccacg
     9841 aaaactgaca acggaatgcg cgcagtcagt ctgcaacaac ttatgacatg gaaggacacc
     9901 cgatggatac ccaacgattc caatcccaat ttcattggca tttatcgttt aaattttctg
     9961 gcgcgatagc agcgtgcttg tccctgtctc ttgtgggaac agggcttgct aatgctgatg
    10021 attcgcttcc ttccagtaac tatgcgccgc ccgccggggg aacattcttt ttgcttgctg
    10081 acagcagttt tagcagcagt gaagaggcga aagtgcgact ggaagcgccg gggcgtgatt
    10141 atcggcgcta tcagatggaa gagtacggcg gcgtggacgt tcgcctgtat cgtattcctg
    10201 acccgatggc atttttgcgc cagcagaaaa acctgcatcg cattgtggtg caaccgcaat
    10261 atctgggcga cgggctgaac aatacgctga cctggctgtg ggataactgg tacggcaaat
    10321 ctcgccgcgt aatgcagcgt actttctctt ctcagtcacg gcagaatgtg actcaggcat
    10381 tacccgaatt acatctcggc aatgccatta ttaaaccttc ccgttatgta cagaacaacc
    10441 agttttcccc gctgaaaaaa tatcccttgg tggaacagtt ccgttatcca ctatggcagg
    10501 ctaaaccggt cgagccgcag caaggggtaa aactggaagg cgcatccagc aatttcatct
    10561 cgccgcagcc gggtaacatt tatattcctc tcggccaaca agagccggga ctgtacctcg
    10621 tcgaggcgat ggttggtggg tatcgggcga cgacggtggt gtttgtttcc gataccgtgg
    10681 cgcttagcaa agtgtcaggc aacgagcttc tggtgtggac cgcgggtaaa aaacagggtg
    10741 aagcgaagcc cggctcagag atcttgtgga ctgacggtct tggcgtgatg acccgcggtg
    10801 tgaccgatga cagcggtacc ttgcagttac aacatatatc gccagaacgc tcatacattc
    10861 tgggtaagga tgctgaaggc ggcgtttttg tctccgagaa cttcttttac gaaagtgaaa
    10921 tctacaacac ccgcttgtat atctttaccg atcgcccgct atatcgcgca ggcgatcgtg
    10981 tcgatgttaa agtgatgggc cgcgagttcc acgatccgtt gcattcatcc cccatcgtca
    11041 gcgccccggc gaagctttcg gtgctggacg ccaacggcag tctgttgcaa accgtcaatg
    11101 tcacgctgga tgcgcgcaat ggcgggcagg gaagtttccg cctgccagaa aatgccgttg
    11161 ccggaggtta tgagttacgt cttgcttacc gcaatcaggt ctatagcagc agttttcgcg
    11221 tggcaaacta catcaagcca catttcgaga ttggtttagc tctcgacaaa aaagagttca
    11281 aaactggcga agcggtcagc ggcaaactgc aactgctcta cccggatggc gagccggtaa
    11341 aaaatgcccg cgtgcagtta agtttgcgcg ctcagcaatt atcaatggtc ggtaacgatt
    11401 tgcgttatgc cggacgtttc cccgtgtcgc tggaaggcag cgaaacggtg tccgacgcca
    11461 gcggtcatgt ggcgttaaat ctccccgccg ccgataaacc gagccgttat ttgttaaccg
    11521 tctccgccag tgacggcgcg gcgtatcgtg tcaccaccac caaagagatc ctcattgaac
    11581 gcggtctggc gcattactca ttaagtactg ccgcacaata cagtaatagc ggcgagtcgg
    11641 ttgtgttccg ttatgccgcg ctggaatctt caaaacaggt tcctgttacg tatgaatggt
    11701 tgcgtctcga agaccgcacg agccatagcg gagagctacc gtcaggcggc aaatccttta
    11761 ccgtcaattt cgctaaacct ggcaactaca atctgacatt acgcgataaa gacggcttaa
    11821 ttctcgctgg gttaagtcat gccgtcagcg gtaagggcag cacggcgcat actggtacgg
    11881 tagatatcgt ggcggataaa acgctgtacc agccaggcga aactgcgaag atgctgatta
    11941 cctttccgga gccaattgat gaagcattat tgacgctgga acgcgatcgc gtggaacagc
    12001 agtcgctgct ttcgcatccg gcaaactggc tgacgttaca acgtttaaac gatacccagt
    12061 atgaagcccg ggttccagtg agcaattcct ttgcgcctaa catcactttt tcggtgctgt
    12121 atacccgtaa tggtcagtac agttttcaga acgccgggat caaagttgcc gttcctcagc
    12181 ttgatatccg ggtgaaaacg gacaaaaccc attaccagcc tggtgaactg gtcaatgtcg
    12241 aattaacctc gtcgctgaaa ggtaaacctg tttctgcgca gctaacggta ggcgtggtcg
    12301 atgaaatgat ctacgcgctg caaccagaaa tcgcgccgaa tatcggcaaa tttttctatc
    12361 cgctggggcg taacaatgtg cgtaccagct ccagtctgtc gtttatcagc tacgaccagg
    12421 cgctctccag cgagccggtt gcgcctggcg caactaaccg cagcgagcgg cgagtaaaaa
    12481 tgcttgaacg tccacggcgt gaagaggtgg ataccgcggc atggatgccg tcactcacaa
    12541 ccgataaaca aggcaaagcg tatttcacgt tcctgatgcc tgattcgtta acccgctggc
    12601 gtatcaccgc gcgtgggatg aacggcgacg ggctggtcgg gcaggggcgt gcttatctgc
    12661 gttcggaaaa aaatctctac atgaagtgga gtatgccaac ggtgtatcgc gtgggcgaca
    12721 aaccggcggc aggactgttt atcttcagtc agcaggataa cgaaccggtg gcgctggtga
    12781 ctaaatttgc aggcgctgag atgcgccaga cgctgacgct gcacaaaggg gcgaattata
    12841 tttcgctggc gcagaacatt cagcaatctg gcttgttaag cgcagaactg caacaaaatg
    12901 ggcaagtgca ggacagcatt agcacaaaac tgtcttttgt ggataacagc tggcccgttg
    12961 aacagcagaa aaatgtcatg ctcggcggtg gcgagaacgc gctgatgttg cccgagcagg
    13021 ctagcaatat ccggctacaa agtagcgaaa cgccgcagga gattttccgc aacaatcttg
    13081 atgcgttagt cgatggacca tggggtggcg tgatcaacac cggtagccgt ctgatcccgc
    13141 tcagtctcgc ctggcgttcg cttgccgatc atcaaagtgc cgccgctaac gacattcgtc
    13201 agatgattca ggataaccgt ctgcggctga tgcaactggc ggggcccgga gcgcgcttta
    13261 cctggtgggg tgaagatggc aatggtgacg ccttccttac ggcatgggca tggtacgccg
    13321 actggcaggc cagccaggcg ctcggcgtaa cgcaacaacc ggaatactgg cagcatatgc
    13381 tcgacagtta tgccgagcag gcggataaca tgccgttatt gcatcgggcg ctggtgctgg
    13441 cgtgggcaca ggagatgaat ctgccgtgca aaacgttgtt gaaagggttg gatgaagcta
    13501 tcgcccggcg cggaactaaa gatgaagatt tctctgagga agacatccgc gatatcaatg
    13561 acagcctgat cctcgataca ccggaatctc cactgggaga tgcggtggca aacgtcttaa
    13621 ccatgacgtt gctgaaaaaa gcgcagttga agtccacggt gatgccacag gttcagcaat
    13681 atgcgtggga taaagcggca aacagcaatc agccgctggc gcacacggtt gtgctgctca
    13741 atagcggtgg tgacgctacc cagacggccg ctattttaag tggtttgacc gctgagcaat
    13801 ccactattga gcgcgcgctg gccatgaact ggctggcgaa atatatggcg acaatgcctc
    13861 cagttgtttt gcctgcgcct gcgggcgcat gggctaaaca taagttaact ggagggggcg
    13921 aatactggcg ttgggttggt cagggtgtgc cggacattct ctcttttggt gacgaattat
    13981 cgccgcaaaa tgtgcaggtc cgctggcgtg aaccggcaaa aacggctcaa caaagtaaca
    14041 ttccggtgac cgttgaacgc cagttgtatc ggcttatccc cggtgaagaa gagatgagct
    14101 ttactctgca gccggtgacc agcaatgaga ttgacagcga tgcgctgtat ctcgatgaaa
    14161 ttacgcttac cagcgagcag gatgcagttc tgcgctatgg tcaggtggaa gtaccgctcc
    14221 cgccgggagc cgacgttgag cgcacaacat ggggcatttc agtcaataaa cccaacgccg
    14281 cgaaacagca ggggcaattg ctggaaaaag cgcgtaatga aatgggcgaa ctggcctata
    14341 tggtgccggt gaaagaactg acgggaacgg tcactttccg ccatttgctg cgcttctcgc
    14401 aaaaagggca attcgttctg cctcctgctc gttatgtgcg ttcctatgca cctgcgcagc
    14461 aaagtgttgc ggcagggagt gaatggaccg ggatgcaggt gaaataagtg aactggcgca
    14521 gaatcgtctg gctattggcg ttggttactc tgccaacgct tgcggaggag acgccgttac
    14581 aactggtgct gcgcggtgcg cagcacgatc aactgtatca gctgtcgtca tcaggcgtga
    14641 ccaaagtctc agcattacct gactcgctca ccacgccgtt agggagtttg tggaaactgt
    14701 atgtttacgc ctggctggag gatacccatc aaccggagca gccctaccag tgtcgcggaa
    14761 actccccgga agaggtttat tgttgtcagg ctggggagag cataacgcgc gataccgcgc
    14821 tggtacgttc ctgtgggctc tattttgcgc cgcagcggtt gcatattggc gcagatgtgt
    14881 gggggcaata ctggcaacag cgtcaggcac ctgcgtggtt agcgtctctg cctacgctga
    14941 agccagaaac ctcggtaacg gttaaatcgc tgcttgattc attagcgacg ttacccgcgc
    15001 aaaacaaagc tcaggaagtc ttactggatg tggtgctgga cgaggcgaaa atcggtgtcg
    15061 catccatgct cggtagtcgg gtacgggtga aaacctggag ctgtttgccc gctatccgct
    15121 gaagaaaatc acagcggaga agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg
    15181 ctatcgtgtg acgtttacca atggtaacca cattacgttt gtcagccacg gtgagacgac
    15241 cttactgagc gaaaagagca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt
    15301 tgccagagtt ctggatcgcg aggcgaaaag tacgccccct gaggcagcga aagccatgac
    15361 cgtggcgatt cgcacttttt tacagcaaaa cgccaatcgg gaaggcgatt gcctgaccat
    15421 ccccgacagc agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac
    15481 gatgaccgcc tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag
    15541 tcgcgctacc gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga
    15601 gcgttacgac cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc
    15661 gccacgctca acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaaagcc
    15721 gcagtggcgg cgtatattac aagctgagac ggggtacaac gaaccagacg tgtttgcggt
    15781 ttgccattta gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg
    15841 taacttcttt acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt
    15901 tgatggttat cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt
    15961 aatggactaa tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg
    16021 tcgttcacag tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc
    16081 cggtagaggg cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca
    16141 acatggcaga tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg
    16201 cggcgggtaa agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa
    16261 ttgaatccga tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc
    16321 aggtcatcag cccggatggg caaagccgac aaaaaatgca gttttactca acgccgggta
    16381 caggaacgat tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc
    16441 tcgatctcca tgttgtcacg cccgatggcg aacacgcctg gtacggtaac acggtgctga
    16501 aaaacagtgg tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca
    16561 tgccagcgcc agttcacgga cgctatcagg tgtatatcaa ctactatggc ggacgcagcg
    16621 aaacggaatt aaccaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga
    16681 aacaggaaac atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt
    16741 ttgactggtg attaatgggg ttaccttcgc tgtcgatgtt caagaaaacg cccgataact
    16801 ttcgctatcg ggcgtttttg ttcgttaatt caaccgttca atcaccatcg caattccctg
    16861 gccgccacca atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg
    16921 taatagtgtg accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc
    16981 cccgccgttg acattcactt tctcaggatc aaagcccaag gttttcccaa cggcaaggaa
    17041 ctgtgcagca aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc
    17101 cagttgtaac gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac
    17161 gccaccgctg gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc
    17221 agattcttcc ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc
    17281 ggtgactgtt cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga
    17341 attcgctttc gggaattcgt cttgactgaa gacgaagatt ttcttccgag tgacaacatt
    17401 taccgggacg atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg
    17461 tgaatgtagc gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt
    17521 ttcggcggta atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat
    17581 tacgtcataa acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa
    17641 gtagggcgct aaactcatat tttccatacc ccccgccaca atgctctgcg cctgacctgc
    17701 ctgaattgcc tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac
    17761 cgtgaatccg cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt
    17821 ttgccccaac ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc
    17881 gatttttgca cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt
    17941 ggaagcgagt gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca
    18001 atttttcatt ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat
    18061 gacgaaaatg acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac
    18121 accgagtccg gcgattgcca gtgctggcag cgcccagaat ggttgtgcca tgttcatcca
    18181 ttgctcgccg taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc
    18241 tgcgggtatc acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa
    18301 gttaatcagt gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt
    18361 gatgaagaat tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc
    18421 agcgtagaag gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc
    18481 acgcatataa gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt
    18541 gacggtattg atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat
    18601 accgagtgcg ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg
    18661 ggcatctttc ggtagctgct tttgaaaatc agcctcttcc atgagtagtt tcggatcgat
    18721 actcaccacg tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat
    18781 caacgccaca gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag
    18841 cccggcgata tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgagccaga
    18901 gaagccgcca ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc
    18961 agaaccgggt actcggcggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa
    19021 tccccagttg atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac
    19081 gggcgtcttt gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc
    19141 aagggcatga ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca
    19201 gaaaccgtca ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc
    19261 gatcacgaat gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccaccg
    19321 gctgacaaaa cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta
    19381 ttcagatcgg cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca
    19441 cacccgtcgg caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt
    19501 tcagtaacca gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gcggcgcaaa
    19561 atttttgctg aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg
    19621 gtcaccagat ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc
    19681 gcgaggtttg cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg
    19741 ccacggatta gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg
    19801 cacggttgcc cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg
    19861 aggaagccgt tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc
    19921 attgtgggta aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca
    19981 cggcgcgcaa tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt
    20041 cgataacggc accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt
    20101 catctggctc taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg
    20161 cgctaagttg ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca
    20221 ggtcggcgcg cagtgggcgt tcgagcagcc aagttttacc gtcgagtgtc agtgtctgtt
    20281 tgccttcctc tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc
    20341 caccacagcg aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac
    20401 cagatatcat gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc
    20461 ggactcgacc attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg
    20521 ctatcaatgt caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag
    20581 tgccaatccc cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg
    20641 cgtcttgtaa tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg
    20701 ggtacagtca ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta
    20761 actaattgaa tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg
    20821 cagaaaattg cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg
    20881 gatcgatacc gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta
    20941 aagcagtgcg ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt
    21001 cgacgcgttt aatttcctct tttaaattac gctcaccgac aggggctgtt tttacctcgc
    21061 cagcattaca gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc
    21121 ctgaattcat cacgacggcg cgttcaataa cgttggaaag ctctcgaata tttcccggcc
    21181 atgaccaggc ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat
    21241 tctcactact gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat
    21301 cgcgcagagg cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga
    21361 aggtaccttc ttttaccatt gcctgcaagt cgcggttggt ggcagcaatg atgcggatat
    21421 caacttttat ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta
    21481 gtaatttggc ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt
    21541 cgttggctcg ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt
    21601 catgaccaaa cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa
    21661 tgaacggccc ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc
    21721 cggtgccgct ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt
    21781 ctttgcagat gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg
    21841 tgctcagtgc ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc
    21901 gctgaacgat taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc
    21961 cgcagcgtag cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg
    22021 gtgtccgggt ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca
    22081 tgcggatatc catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc
    22141 gtccgttgtt cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac
    22201 ggcgaacatt atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg
    22261 atttccctgc gggttgatcg gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact
    22321 ggcgacgcga atatcaccct gatgggcatt aatgatgcgt tgacttaacg ccagaccaag
    22381 cccggttcct gaggctttgg tggtgaaaaa gggatcgaag atcttttttt gcagcgagag
    22441 atcaatgcca cagccgttgt cctctatcga aatggcctgt tgtgagtcgc tgtattgcca
    22501 ggtttgaatg cgaattttcc ctcgtgcgct gatagcctgg acggcattga tcaggatatt
    22561 cagtagtacc tgtttgagca gttcacgatc ggcgttaatc gggcttaatt cattatccag
    22621 ttcgcttatg aagtcgaccc gcgcttgtac gccggcggtt tgtaccagta ccagagtttc
    22681 ttcaaccaat gcattgaggc tgacttgttg ccattgactg tggcgtggac gtgaaaattc
    22741 gagcaattgc tgaataactt tgttaattga atcgatttct ttgagtacta cggacagata
    22801 ttcctgatgt attgggtcac tggtttgttg gcgcaagatc tgtacataac cacgaatagc
    22861 cgttaacgga ttacgtactt catgcgcgac gccagccatc agctcaccca gtgtggcgag
    22921 gcgttctgct tgcgccatgc ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat
    22981 caccaaagca cctatcattt caccgtgcgt gttatgaata cgactggtag tgacactgag
    23041 ttcaatggtg cggtcacgac ctggaaaact gatctccagc gccacatgtt cggtgccatg
    23101 ttccagcgta tccagtactg gactgtagaa ctgagtattg tcgaacaaca tggagtaagg
    23161 ctgccctacc agttcatggc gttgatagcc agtgataact tctgctgctg ggttcatggt
    23221 ggttacatca ccctggcggt caatggcaat gacgccatcg gcagcgtttt caataatcag
    23281 atcgttaagt gtccgcgttt cacgcagtgc ctgggcgagg ttattaacac tctgactgat
    23341 ttgccccatt tcaccgggca attgtggtaa tcgagtggga atattttgtg ccagagtcga
    23401 gaggccatcg gtgatgatat caatattggc gctcaggcga cgggagaaaa ggacaatcaa
    23461 tagcaggctt atcagcaaac cggcggtgag cacaatgata atcctcacat ccattttcca
    23521 ggcctggcgg cgaatatctt cggttaattc attggcccag atatagccga ggatttcacc
    23581 attacgctca atggggagca ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata
    23641 aaccaaaggg gtatttgtac gcatgacttc gcgaccaggg tgatctgcgg caatggtaac
    23701 gccgacatta ttctgatata gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt
    23761 gttgtaataa ccagcaccga tgccagggaa ggcgtgagtg atattttcgg taatgggggc
    23821 aagttctgca tttaatgcgc ggatacgctc ctcacgtggt aagtcgatgt agagatcata
    23881 gcgatcgcct agtgcctgat taagcaggtt gaccacggca gaaagttttt tctctttttc
    23941 agataagact gctgaacgtc cttccgtttc tacgatataa ccaatagtaa gcgttgggac
    24001 aatgaccatc aggattgcca tcaggatcat ttgattgcgt aagcggcgtg gataaatcca
    24061 cttcatataa tgcatgctga aatccactag tcttgtccgg tatatgacga ttatcagagg
    24121 ttaaggtgat gatttctcgg cggtgtatca tattccagag aagagagaac attgcggtaa
    24181 cacgctttta ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc
    24241 tttgaaatac cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc
    24301 attggcgtta gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc
    24361 gttacatcag cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca
    24421 gcgctttatt caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg
    24481 cttatcggca gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc
    24541 tgcgtttgaa ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg
    24601 ttctctggag tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta
    24661 cgatctcaac cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct
    24721 gcgtgatatg ccagtggaac gcgataccgc gttgaaagct ttgcatgaac gcatcaataa
    24781 atatcgaaat gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc
    24841 gcgccctggc gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca
    24901 ggcgcttttg ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt
    24961 gccgatgggg gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc
    25021 agaaagtaaa attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac
    25081 ggaagggttc cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt
    25141 gtattcggtg ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat
    25201 tttgctgaat gtgcttgccg gagctgcatt gtttactctc gcgcggatgt acgagcgacg
    25261 tattttcatt ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg
    25321 caagattgtc gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa
    25381 tattttaagt aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca
    25441 acgactgacg caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa
    25501 caataccaat ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat
    25561 ttgtgtgctg gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc
    25621 acaagcagcg gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga
    25681 gctgcgaacg ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt
    25741 accgaaaggc gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa
    25801 aattatcagc gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc
    25861 gcgtgagttt tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt
    25921 ggtacgcaag cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa
    25981 tggcgacccg atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt
    26041 caccgatacc ggctgtatag ttttgcatgt ccgcgctgat ggcgattatc tctctatccg
    26101 tgttcgcgat accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt
    26161 cttccaggtc ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat
    26221 ttgtgaaaaa ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat
    26281 gggcagccag tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg
    26341 cgtagaaggg ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca
    26401 gttcctggaa accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga
    26461 accgactccc gaagatgtgt taattactga cgaggtagtg agtaaaaaat ggcagggtag
    26521 agcggtagtg accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga
    26581 gtgggtacac agtgtggctg ccccgcatga gctaccggca ttgttggcgc gtatttattt
    26641 gatcgagatg gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag
    26701 cgataatgac gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct
    26761 ggcagatcag ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc
    26821 gcttaatgta cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa
    26881 tatggatggt taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat
    26941 cggagtaact gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga
    27001 cagctgcctg tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc
    27061 cgagagggtc aggaaatcgc gggaatcgta ggacggataa ggtgtttatg ccgcatctgg
    27121 cattcagtgc aaatgccaga tgcgacgctg acgcgtctta tctggcctac tttaatgtaa
    27181 tctgttgaaa taatgggaat cgtaggccgg ataaggcgtt tacgctgcat ccggcaatcg
    27241 gtgcaaatgc cagataagac actaacgcgt cttatctggc ctacagatga ttagtcttta
    27301 tctgccggac ttaaggtcac agaagagaga taattcagca gggcgatatc gttctcgaca
    27361 cccagcttca tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc
    27421 tttttagcga tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct
    27481 tttggcgaga gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa
    27541 acgctttccg gggtaaattt cttccctttc tgcagcgcag cgagagcttt cggcagatcg
    27601 gtcggtgcac cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga
    27661 atcgccgggt tgttgttcat ggtcagaaca atgatcgaca ggcttgggaa atggcgcttg
    27721 atgtacttga ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta
    27781 atcaacacat gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat
    27841 tcgccgacaa cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact
    27901 atcggatggt catcggcaat aattacgttc atattgttca tgtacttgta cctatgtatt
    27961 aactatttga ttttaaaaat atattctatg ctttgtttag tcgttggggc acctgtggga
    28021 cataagtaca tagctttctg tttagtactt ctacctggga ctggttattg tctttcatcc
    28081 atgcaccgta aacattgtaa accatctggg cattcgcatg tcccatctgg ttggctatga
    28141 agtttggatt tgctccggca gttaaagccc agcaagcata agtatgccga gactgatacg
    28201 cttttctatg gcgaattttt gcccgtttta atgcagattc ccatgttctg ttgatggatg
    28261 ttacggcata atgaactcct gagttacctg tgcgatctaa tatctgagga ttgaaaacaa
    28321 agctgcatga gtgagtgctt gtccggccat actctcgcag ctttacttct atctgatatt
    28381 gtttaccaag cctggttaat gtcgcctgat tttttaatac gtcaattgct ggctgtatga
    28441 gttgtattac cctgtttgtt cctgcatctg ttttgggtag tgtaaattcc tttgtctgag
    28501 tgtaattcct tttgacggtc aaggtacccg cttccagatc gatatcctcc catgccagcc
    28561 cacacagttc gccatgccgc atcccggtat atacagccag cgaccagaaa tttttagttt
    28621 gctggtggtg gcacgcatca atcagtctgg caaactcatc ttttgttagt ggatcgggag
    28681 gtgttttcgc tcttttcagt gatggaagct cactgaaagg gtttttgcct atatacccat
    28741 tgtttgccgc aaaacggaac atcccggaga taattgtcat atagctattt acagtaggta
    28801 cactacgacc tttaataggc ttcctctgat tttttcctgg caactgatat cccgttaata
    28861 attctttacg aaaaatgaga acatcttcct gtgtaattga tgatgccagt ttgttccctc
    28921 caaggcagtc aaggcaattt cggatagctg atttgtatct gaccatggca tttgctgtta
    28981 tttccatttt cttaagctca agccattttt ccgaaagtgc cttaatggtt atctcttttt
    29041 ttcccagacc aaaatgtttc aggttagggg aattagggaa ctgcgcggcg tagtcgaagc
    29101 tccccattct gattgcaaaa caaacggaag tgcgaagttc acctgcgatc ttccggtttt
    29161 tggcggtgtc aggaacaccg aggttttctc tgacacgttt gccgttatag tgaaaccata
    29221 tgcggagtga tccgccatgg ttttcaacgc ctgtcgggta tgatgcgtta ctcattaaac
    29281 ctcccagacg tccaggagca ttaacaggtt aaccggaact tgcatttttg gcacctggtt
    29341 gtttctggtt ttcgatccag cgcataattt cctcaatgtt gtatacacat tcactgaaag
    29401 gcccgggctc tccttctgct gcgtaatgac ggtattcttt ccccagtaac caggattttt
    29461 ttcttgctct ggcaatggtt cctgatttca gtccagttgc ggcaataagg gttctttctg
    29521 tgcaccattt gctgggcgtt atctgataga tgattgtttc cattcctgac tcacactatc
    29581 ttcaggccac ggcagtggca ccacacgtca aacattcgct ttacaacttc acggcagtag
    29641 aagccgtgag catctctggt taaatcgtat cggtacccgt atctgagtcg tatccatatt
    29701 tcaaactcgc gattcacgac ttattctcct gctgtcttta tgccagttca ttcggttgcg
    29761 ctggggccct ggccttccgt ggcctgtatt tgcggcgttt tctggcttca atgcgtatct
    29821 ttgtcaccct gactggcttt ggttcgtgcc ggaacacttt gtttctgata ttacgccgtg
    29881 tattcatcat ccagataaaa cgctgggtat ggtcgcatcc gtcatcaacg gtaattactg
    29941 tgcggaccag ttcatcgtta atatcaacca tttcacccat tcactgagcc ctcatacgcg
    30001 aatttcgtgg cttccatcag ttgcgtccat acccattcaa gaaattgtgc gttttctcct
    30061 gagcggtcat attcatcgac gctgcggggc ttgatggtcg ccagtagtgg cacaatccgg
    30121 tacttcatct gttcggttaa cttgtccggt acaagtcgcc agccgttata tttcgatgga
    30181 acatggtggt attcagccag gaagcgtcgt atatcgtttt tcatttcctc gcatgagttc
    30241 tgatcatgaa gtgcagcgga aagccagcgt ccgatatatt cagggattcg cacatcaagt
    30301 actttttctg ccgtcgtcag cattgcctgt cggcaggcgt tccagccgtt gacatagtga
    30361 cgtgttgcat cccatgaaac ctgcacatca gggtaagcct cctgttctgt tctcactggc
    30421 ggtatctgag gaacagtgaa tagtggactg acgatatatt tatccagaga gcctccccgc
    30481 tcttctgcaa gactttccca cgatgtcatt gtggggaccc tgtaacctgc ttttttgtgc
    30541 gtatacatcc atgccactct gccgttatca tctgcggcgg caatgcgctg ccaggcagga
    30601 taaagcgggg tgtatgtttc tctgtcatta cgggcgtcag gcatggcatc aatactgcaa
    30661 acttcccccg taaggttatt cagccaggcc ttcgggctaa tctctgacgt tacgatttca
    30721 tccagttttt tctccagatt ttctatgtga ctgattagcg caaggataac tggcggagtg
    30781 gcaagggagt gaaatgcttc cagatcacat cccttgtcgc catatacggt attttgtgcg
    30841 gcgtcacgca gtgctttcag atcagcttct ttcatcgttc agactccctt gttgctgttt
    30901 tgtaggcgcg caggatgtcg cgtgtttttc ctgataatga cgacttaacc aggaaaaagc
    30961 cgctccggct ttctgtgatg tccggtgtgc acacacagaa gtaccgcatc aacgacacga
    31021 ttatgttttc gcaatgtcag gcgggaactg gtgataatca gttttgcctg ttcgccgtaa
    31081 tcggtgaatg aaataatcat ctgatggcga tctccgttat gaaagtgttc ctgctggttc
    31141 aatgctgata atttctgctg caataagcat acctgccagc catgcttcgc cgagcatgtc
    31201 ctcatccgta catgcaagat caccgatctg tattgtggcc ataatgttag tttcaccggt
    31261 atattcatcc tgtgcttctt catacggcag gtcttcataa aggttttcaa ttgcgcaggt
    31321 tattacatcc agtcccgtca gattgccgac aacgtccact tcaaatgttt cgcggtactc
    31381 ccattgcccg agtgtcagac gaaccgtctg tcttgccatg cggccgcata atgtcatgtt
    31441 cgggtcgtag ttcatgattg ccgcagtagt gctattcatg ttctgcctcc ggtgtgtaaa
    31501 tagctttgtc gtgtttaaat tcaccgttcc agtttttctt catagggagt tcaccgttca
    31561 tgtaaaactg gtataaacgg tggcatcctt tttccagcaa tacgggggtg aatttcgtga
    31621 atgttgcttt cccgtgtgga gtgatctgta ctgcttcttc ggtcaggtat ctgtcacggg
    31681 cataagaggc cgtgcgccag cggggtgttt tctccggatc gcgttgttcg ttgaataccc
    31741 attttcggcc agacagccac cacattatct ggctggtatt tacgccgttc agcgcacggc
    31801 agaactgggg aacagtcatg cctttagaga aatgtttttc cagactgtct acggttgcag
    31861 agagcgtttt attttccagg gccatagctt cagcccgttc ttcggcctca ataaccatca
    31921 acgcgagttc cttgcgagtg agtacgcgct gaacatcgat gttttcccgc tgagtgaagt
    31981 agaactccac aaggtcttcg tgatagttcc acgcctgatc cgtttccagc aattttgcgt
    32041 ggttggctgc gccgcgttcg gtccagagga tgagggatcg aacattgctg gcaattttca
    32101 cagagtagct ttgagatact ctgtgcttaa gttcacgtag ttcatcgcct tccagcaaaa
    32161 aataatgctt tccaacaacg aatcgttctt cgtttcggga atgattctgg cgaatacgga
    32221 ttggtgttgc tccatagcct gccgccagtt gttcagtggt tataacgcgt tgaccgcgat
    32281 attcgataat ttgcaaatca cgggctgcca ccggcacaag ttcagttgat tttttcatca
    32341 ttacacctca cgaaaagctg attagtggaa cactctggtt tccggttctg agttgtcagt
    32401 ggcttcactg ataagcccat ctagaatcat gtgcatcgct ctgtaacctt ccattgtgat
    32461 gccggttttt tcgccgtcgc ccagagcgcc ggtgaatgct ttgtacataa gaatgctttt
    32521 gagcagccct gattccgctc cgtaagtttc tatgagtgcc cattccatat agttctgcat
    32581 agcgagacgt aatggtttca ggtagattgt catgccaccg ttgtggcctc tgtacatggt
    32641 gcccttgtct gtaccgccat tctcgttaag cacttcggta cggccgtttc tggtgagctg
    32701 ttcagagaag aacatggcgg tataaagcca gcgccagtac atgattgtct tttccggcgc
    32761 gagctcacct gtcatgcctt tttcttctgc ttcactgatg caacacacta tttccagacc
    32821 atgccagatc ggttcgtcaa aacagccttc atcgagcatt tccagtgctt tgttgatatc
    32881 aagcagtccg taaggtgtgt ggataccttc agcagtaaga aaaaataact gttcagttgc
    32941 tgtcatggtt tgctctcttc ttaaatggtc tttgcgcgac aagccggcaa tactgatcaa
    33001 tccaggcgtt gtatttttcc atccaggcat caaactgttt tctgcgtgca aggatgcgca
    33061 acaggcgatg aaaacaacgc ccgtgagctc tgtagtattc gggcgtaatt tctcccggat
    33121 gaaagacttc tccatattcc ggatgcatca ggcgctggtc aggcagacgt ttttcaaaac
    33181 ctgaactttc gaataccttc gttgtcataa aatgagcgag atgtatgatg gccgtttttc
    33241 gcgtgaagca tttcttaact tgtccgtggc ggcagactct gtaaacaggg ccgcaatcga
    33301 gttcgtattt atgaaaagca cggtcgattc cggcattatt tattgcactg tattccgtat
    33361 cactgaagaa aggtggaaat acgatgtatc cttccattgt cagcgccaga acgcgcggat
    33421 cggtttctgt cagccattcg ttttctgtga attcttcaga attaatttca atgatttcag
    33481 gttcttcttt catggttact ccgtgttcat ccggtgttaa ttacttttat tcactctgag
    33541 cgcagccaga agatgtggct cagcaattcg tttgttgcga tatatttgtg atacagcctg
    33601 gccttttcag gttgtaaaaa tccccgacaa taaagctgta aattaattac tgttcttaaa
    33661 taatgttatt cgttttcagt ccggcacaga tgcttttctg ctttgtctgc tgtacaggat
    33721 aatgagcgga aaagacaatt catcatgttt agcgcatctg gtgaaccaat atcaccgctg
    33781 tcaataatca cctgtaacaa agacaggctt tctgttatct cgcagtggac attatcgagt
    33841 acttcaacgc aattattcat ttttcattat ctccgttatt ccgggggagg agctatgctg
    33901 ttaatgatta acgcatccgg taatatgccc ctctttaatg taatcgatgc attcattcaa
    33961 aagttcatca atgattaact ttcctgattc agtcagatac tcaccatgtt ggttgatacc
    34021 gacagcatcc tggtatgcag tgcgtattgc tgtttcacca tctttccgcc caaactcacc
    34081 acgggtaacg ccttcaaatc gtaatagcaa ctggcttatg aactgttctg ttatttcaat
    34141 agttgttaac tctccatcat gacgctttac agtgaggaga ttacttcctg tttttcgttt
    34201 catccgccgc agtgctgcaa ccgttatgcg gcgacgatat gcagaaatgt attcatcttt
    34261 catttgttat ttcccgtatg cctttttgag aaacaatact gcaactgacc agtatccggc
    34321 atcagccatt aataaagcgg ttttataggc atgtttgtct ttcatgtgtc atccccattt
    34381 taaaatagtg agaactcccg accgtagacc gtcatgtttt tatgtatgat tgtctgttta
    34441 atttccttta ttcgttctca gagcatcaac atactcgtaa gccttttcac aggttttatt
    34501 catggaacga atcagacaaa gtaagaaatc atctgtctct ccgttatcac cagagttttt
    34561 aaatataaac tcaagcattg acgtgttttc ttttatttct gctgccactt cctcaagcat
    34621 aatcagggga gttttcatgt tctttgttcc ttaaatgcat cgcatgcgct tctggcgtat
    34681 tgttgtgcca gtaaaaagat gtcatccgaa agttcatcac attcttcatc accggaagcc
    34741 gaaatgatta accccgcttc aagcagtact gcaatgtgat gaaaagctgt tttcggttcg
    34801 ttggtgaggc ctttgaacat tttcatctta cgattccttt gtcgtgtcag aatggaaact
    34861 tgtaagtaac cacatcggat cgcagtcaag tatatttgcc agaggaatca cctggcttgc
    34921 cagaggttca gccttgccgt tctcccactg gatgatggtt tcttcatcaa ctccaagcag
    34981 cgtggcgagt tcagcggtgg atagaccgca gctttcgcgt tgggtgcgga tgccatcacg
    35041 gcaatctttt ttgtttctcg gaggtaacag aatatgtttt agttgttgga ttttttcgat
    35101 tccaacctca aggagtacgt gacggtttct ttccgataca gactcgagac tttcatgaag
    35161 cagcgattct acttgttcga taagcatcaa ttgtgaagta gacataaaaa aacctcgtaa
    35221 ccaatatttc gaaatctagg ttacgagtga gtaatgtagg tgtcaagagc aattaatgtt
    35281 ttttgttgaa ttaaatttgc atacgcatct caacgacaac accaataatt tcgcaatccc
    35341 cattgatggg gatcagtgga tagcgtgggt ttagtggctt taggaaacat tgcccagcgt
    35401 cttctatgta ctgtttaagg gttgcctctc cggagttttt gagcctggcg acgacatatt
    35461 ttcctgaaaa aacttctttg tcagggttaa caagaatgtt catgccttca aaaatagaga
    35521 actctccatt ctgtgatgtc attgagtccc ctttcacttc cagccagaag ccgtgatcgc
    35581 ccgcatatat atcactacca atccattctt ctggataagc tatgtaatct tcgcagttcc
    35641 aggggccagc actcacccat gttagtttcg gatatctata acggtttgga gaaactggct
    35701 taggatttcg gacgttttga atgctatctg taggtgatgg ggcgctgccg tcaagaagcc
    35761 atttaggaga gcatttcaat gcatccgcga ttctaaatag gtttgaagat cttgtgtctt
    35821 gtgagtctcc taactctatt ttgctgattg ttacacgcga gacaccagtc aattttgcca
    35881 ggtcatcttg tgacattcct agctgtactc tgcgcattaa cagtctgcca ccaaagctat
    35941 cgtgatccat cttctccccc ttcttgtcga aataatcgtt tcgaaacaaa tatagcaaaa
    36001 aatcttgaaa tctaggtttc ggaaaattaa gattaatcga aacaaacgtg tcgaggtgga
    36061 tatggatttg tatgaaattc taaaaaacat gtttggatcg aatgttgaaa taggtcgtta
    36121 ttttccccga aggggaaggg ctagaaccgg acaggctgta ggtaagtgga aaacccgagg
    36181 tgtgccggaa gatgtggcga tcctttgtca tctggatccc aaaattcctt atcagcatcc
    36241 gtcgctcatg aatgccagtc atgaatcgtg atgttctgcc ggaggctgta tgtcccctga
    36301 ttacgttcag atggagatgc ctgccaggta cagccaggca gattcagagt ggattaagcg
    36361 gcagttacag agcctacctt cctcactgag acagaaggtc gccctgaaat acgcggaggt
    36421 ttacgaagtt acttttgatt ccgagcctgt ttcattccgt aaggagaaca gagcgaggca
    36481 tgaagccaat gtacggctgc gcaggttcgt tgaaacacac gggcgtgcat tacaggggta
    36541 tacgactcag ccgcccctgg caggaacgca acagcgtacc tgaatggtgt tggtcttaaa
    36601 ggtgaccgac gggctgagac cccacttctc aggatgcacc tgtgtactac caa
//
LOCUS       DAZGAN020000040        36148 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGAN020000040 DAZGAN020000000
VERSION     DAZGAN020000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 36148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.040"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(94..915)
                     /gene="bacA"
                     /locus_tag="ACHKYO_RS11015"
                     /old_locus_tag="pgaptmp_002203"
     CDS             complement(94..915)
                     /gene="bacA"
                     /locus_tag="ACHKYO_RS11015"
                     /old_locus_tag="pgaptmp_002203"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU4429051.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(1005..1373)
                     /gene="folB"
                     /locus_tag="ACHKYO_RS11020"
                     /old_locus_tag="pgaptmp_002204"
     CDS             complement(1005..1373)
                     /gene="folB"
                     /locus_tag="ACHKYO_RS11020"
                     /old_locus_tag="pgaptmp_002204"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HGU4429052.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            1478..2095
                     /gene="plsY"
                     /locus_tag="ACHKYO_RS11025"
                     /old_locus_tag="pgaptmp_002205"
     CDS             1478..2095
                     /gene="plsY"
                     /locus_tag="ACHKYO_RS11025"
                     /old_locus_tag="pgaptmp_002205"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGU4429053.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(2108..3040)
                     /gene="ttdR"
                     /locus_tag="ACHKYO_RS11030"
                     /old_locus_tag="pgaptmp_002206"
                     /gene_synonym="dan"
     CDS             complement(2108..3040)
                     /gene="ttdR"
                     /locus_tag="ACHKYO_RS11030"
                     /old_locus_tag="pgaptmp_002206"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HGU4429054.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            3247..4158
                     /gene="ttdA"
                     /locus_tag="ACHKYO_RS11035"
                     /old_locus_tag="pgaptmp_002207"
     CDS             3247..4158
                     /gene="ttdA"
                     /locus_tag="ACHKYO_RS11035"
                     /old_locus_tag="pgaptmp_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HGU4429055.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            4155..4760
                     /gene="ttdB"
                     /locus_tag="ACHKYO_RS11040"
                     /old_locus_tag="pgaptmp_002208"
     CDS             4155..4760
                     /gene="ttdB"
                     /locus_tag="ACHKYO_RS11040"
                     /old_locus_tag="pgaptmp_002208"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HGU4429056.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            4808..6271
                     /gene="ttdT"
                     /locus_tag="ACHKYO_RS11045"
                     /old_locus_tag="pgaptmp_002209"
     CDS             4808..6271
                     /gene="ttdT"
                     /locus_tag="ACHKYO_RS11045"
                     /old_locus_tag="pgaptmp_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HGU4429057.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(6314..7327)
                     /gene="tsaD"
                     /locus_tag="ACHKYO_RS11050"
                     /old_locus_tag="pgaptmp_002210"
     CDS             complement(6314..7327)
                     /gene="tsaD"
                     /locus_tag="ACHKYO_RS11050"
                     /old_locus_tag="pgaptmp_002210"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGU4429058.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            7565..7780
                     /gene="rpsU"
                     /locus_tag="ACHKYO_RS11055"
                     /old_locus_tag="pgaptmp_002211"
     CDS             7565..7780
                     /gene="rpsU"
                     /locus_tag="ACHKYO_RS11055"
                     /old_locus_tag="pgaptmp_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGU4429059.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            7891..9636
                     /gene="dnaG"
                     /locus_tag="ACHKYO_RS11060"
                     /old_locus_tag="pgaptmp_002212"
     CDS             7891..9636
                     /gene="dnaG"
                     /locus_tag="ACHKYO_RS11060"
                     /old_locus_tag="pgaptmp_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGU4429060.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQKPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            9831..11672
                     /gene="rpoD"
                     /locus_tag="ACHKYO_RS11065"
                     /old_locus_tag="pgaptmp_002213"
     CDS             9831..11672
                     /gene="rpoD"
                     /locus_tag="ACHKYO_RS11065"
                     /old_locus_tag="pgaptmp_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGU4429061.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSNAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(11750..12256)
                     /gene="mug"
                     /locus_tag="ACHKYO_RS11070"
                     /old_locus_tag="pgaptmp_002214"
     CDS             complement(11750..12256)
                     /gene="mug"
                     /locus_tag="ACHKYO_RS11070"
                     /old_locus_tag="pgaptmp_002214"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HGU4429062.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            12381..12456
                     /locus_tag="ACHKYO_RS11075"
                     /old_locus_tag="pgaptmp_002215"
     tRNA            12381..12456
                     /locus_tag="ACHKYO_RS11075"
                     /old_locus_tag="pgaptmp_002215"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12414..12416,aa:Ile,seq:cat)
     gene            complement(12510..13274)
                     /gene="nfeF"
                     /locus_tag="ACHKYO_RS11080"
                     /old_locus_tag="pgaptmp_002216"
                     /gene_synonym="yqjH"
     CDS             complement(12510..13274)
                     /gene="nfeF"
                     /locus_tag="ACHKYO_RS11080"
                     /old_locus_tag="pgaptmp_002216"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HGU4429063.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            13551..14174
                     /gene="nfeR"
                     /locus_tag="ACHKYO_RS11085"
                     /old_locus_tag="pgaptmp_002217"
                     /gene_synonym="yqjI"
     CDS             13551..14174
                     /gene="nfeR"
                     /locus_tag="ACHKYO_RS11085"
                     /old_locus_tag="pgaptmp_002217"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HGU4429064.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(14328..15848)
                     /gene="aer"
                     /locus_tag="ACHKYO_RS11090"
                     /old_locus_tag="pgaptmp_002218"
     CDS             complement(14328..15848)
                     /gene="aer"
                     /locus_tag="ACHKYO_RS11090"
                     /old_locus_tag="pgaptmp_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HGU4429065.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            16266..17645
                     /gene="ygjG"
                     /locus_tag="ACHKYO_RS11095"
                     /old_locus_tag="pgaptmp_002219"
     CDS             16266..17645
                     /gene="ygjG"
                     /locus_tag="ACHKYO_RS11095"
                     /old_locus_tag="pgaptmp_002219"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HGU4429066.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(17687..18019)
                     /gene="ygjH"
                     /locus_tag="ACHKYO_RS11100"
                     /old_locus_tag="pgaptmp_002220"
     CDS             complement(17687..18019)
                     /gene="ygjH"
                     /locus_tag="ACHKYO_RS11100"
                     /old_locus_tag="pgaptmp_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HGU4429067.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            18238..19221
                     /gene="ebgR"
                     /locus_tag="ACHKYO_RS11105"
                     /old_locus_tag="pgaptmp_002221"
     CDS             18238..19221
                     /gene="ebgR"
                     /locus_tag="ACHKYO_RS11105"
                     /old_locus_tag="pgaptmp_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HGU4429068.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            19405..22497
                     /gene="ebgA"
                     /locus_tag="ACHKYO_RS11110"
                     /old_locus_tag="pgaptmp_002222"
     CDS             19405..22497
                     /gene="ebgA"
                     /locus_tag="ACHKYO_RS11110"
                     /old_locus_tag="pgaptmp_002222"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HGU4429069.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTHGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            22494..22943
                     /gene="ebgC"
                     /locus_tag="ACHKYO_RS11115"
                     /old_locus_tag="pgaptmp_002223"
     CDS             22494..22943
                     /gene="ebgC"
                     /locus_tag="ACHKYO_RS11115"
                     /old_locus_tag="pgaptmp_002223"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HGU4429070.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            23006..24439
                     /gene="ygjI"
                     /locus_tag="ACHKYO_RS11120"
                     /old_locus_tag="pgaptmp_002224"
     CDS             23006..24439
                     /gene="ygjI"
                     /locus_tag="ACHKYO_RS11120"
                     /old_locus_tag="pgaptmp_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4429071.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            24573..25643
                     /gene="ygjJ"
                     /locus_tag="ACHKYO_RS11125"
                     /old_locus_tag="pgaptmp_002225"
     CDS             24573..25643
                     /gene="ygjJ"
                     /locus_tag="ACHKYO_RS11125"
                     /old_locus_tag="pgaptmp_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HGU4429072.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            25660..28011
                     /gene="ygjK"
                     /locus_tag="ACHKYO_RS11130"
                     /old_locus_tag="pgaptmp_002226"
     CDS             25660..28011
                     /gene="ygjK"
                     /locus_tag="ACHKYO_RS11130"
                     /old_locus_tag="pgaptmp_002226"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGU4429073.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            28337..30355
                     /gene="fadH"
                     /locus_tag="ACHKYO_RS11135"
                     /old_locus_tag="pgaptmp_002227"
     CDS             28337..30355
                     /gene="fadH"
                     /locus_tag="ACHKYO_RS11135"
                     /old_locus_tag="pgaptmp_002227"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HGU4429074.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVFAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(30400..30816)
                     /gene="higA"
                     /locus_tag="ACHKYO_RS11140"
                     /old_locus_tag="pgaptmp_002228"
     CDS             complement(30400..30816)
                     /gene="higA"
                     /locus_tag="ACHKYO_RS11140"
                     /old_locus_tag="pgaptmp_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HGU4429075.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(30813..30896)
                     /locus_tag="ACHKYO_RS11145"
                     /old_locus_tag="pgaptmp_002229"
                     /pseudo
     CDS             complement(30813..30896)
                     /locus_tag="ACHKYO_RS11145"
                     /old_locus_tag="pgaptmp_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            complement(31087..32223)
                     /gene="rlmG"
                     /locus_tag="ACHKYO_RS11150"
                     /old_locus_tag="pgaptmp_002230"
     CDS             complement(31087..32223)
                     /gene="rlmG"
                     /locus_tag="ACHKYO_RS11150"
                     /old_locus_tag="pgaptmp_002230"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HGU4429076.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            32308..32811
                     /gene="ygjP"
                     /locus_tag="ACHKYO_RS11155"
                     /old_locus_tag="pgaptmp_002231"
     CDS             32308..32811
                     /gene="ygjP"
                     /locus_tag="ACHKYO_RS11155"
                     /old_locus_tag="pgaptmp_002231"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HGU4429077.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            32888..33571
                     /gene="ygjQ"
                     /locus_tag="ACHKYO_RS11160"
                     /old_locus_tag="pgaptmp_002232"
     CDS             32888..33571
                     /gene="ygjQ"
                     /locus_tag="ACHKYO_RS11160"
                     /old_locus_tag="pgaptmp_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HGU4429078.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPERNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            33650..34636
                     /gene="ygjR"
                     /locus_tag="ACHKYO_RS11165"
                     /old_locus_tag="pgaptmp_002233"
     CDS             33650..34636
                     /gene="ygjR"
                     /locus_tag="ACHKYO_RS11165"
                     /old_locus_tag="pgaptmp_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4429079.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            34919..35884
                     /gene="alx"
                     /locus_tag="ACHKYO_RS11170"
                     /old_locus_tag="pgaptmp_002234"
     CDS             34919..35884
                     /gene="alx"
                     /locus_tag="ACHKYO_RS11170"
                     /old_locus_tag="pgaptmp_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HGU4429080.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         8661 a         9664 c         9403 g         8420 t
ORIGIN      
        1 agaccagcac cggtgcgaac aatcaccccc accccggccc tctccctaca gagcgaggga
       61 gaaaaccagg cctgctcccg aaccttttct caatcaaaag aacacgacgt acaccgcagc
      121 cgccacaata aagcgataaa tggcgaacgg gataaacgaa atgcgcttaa tcaattgcag
      181 gaaggtttta atcgctatca gcgccaccac aaaagcggtg ataaacccaa cggcaaacat
      241 cgggatatcg ccggttgtca ggaagcccca gcttttgtag agatcgagcg ccgttgcgcc
      301 catcatcatc ggtaccgcca gcaggaacga gaactcggaa gcagcgtaac ggctcacgcc
      361 catcagcatc ccacctgaaa tggtggcccc ggaacgggag aaacccggcc acagcgccag
      421 acactggaaa cagccaatca taaacgcctg acgataggtc atatcatcaa gacccggcgc
      481 acgcggctct ttcggcttca ggcattcggc ggcaatcagc aacaaaccgc caacgaccag
      541 tgcatacatc acatttatcg ggttaaacaa tgacttaatc gtgtcgtgga acaacagccc
      601 cagtaccacc gccggaatca tccccagcaa aatgtggatc agcgttaaac gacctttgct
      661 ttctccttcg tgctgcaacg ggcggccaaa gtggatgcca atcaggccaa acagacgccg
      721 ccagaacatc actactaccg ccagaattga tcctaactgg atcacaactt caaaggtttt
      781 cgccgtgtcg ccctcgaacc ccaacaagtg accgacaata atcatatggc ccgtgctgga
      841 taccggcaga aattctgtca atccttcgac cacacccaat attgccgcta tcagcagcga
      901 gtgcatatcg ctcatcaata aacccctaaa ttattaaaat gtaccgcttg tccgaactac
      961 tgcgtatgac caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc
     1021 agattattgc cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc
     1081 ttgctgagtt tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc
     1141 tcttcagcca cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt
     1201 tctgcaatgt cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg
     1261 ttatcccatg ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg
     1321 taaacaccaa tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc
     1381 tgcctgcttt ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat
     1441 tcatcgttga acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat
     1501 gatcctcatc gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg
     1561 tgggctgccc gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg
     1621 tatcggtggc aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt
     1681 gcccgtctgg ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat
     1741 cgccgcctgt cttggacaca tctggcccgt tttcttcgga tttaaaggag gaaaaggcgt
     1801 tgctaccgct tttggtgcca tcgcacccat tggctgggat ctcaccggag taatggcggg
     1861 aacctggtta ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc
     1921 actgattgct ccgttttatg tatggtggtt taagccacaa ttcaccttcc cggtttcgat
     1981 gctctcttgc ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca
     2041 ggagacaaaa atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct
     2101 ggtggatcta catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg
     2161 ccgccagaaa ttccacgcag acacgtaatt tcatgctgcg atagagcggc tcccggtaaa
     2221 cagcccagat attggcgctt tgtgcatact ccggcaatac ttgcaccagt ttgccactct
     2281 ccagaaacgg caacacatcc cactcggaac gcagcataat ccctttgccc tccagcgccc
     2341 attgcaacac tatctcgccg ctattggagg aaagatgccc gctaaccttc accgattttt
     2401 tctcctgacc gttccccaac tcccatattc cgtgggtcat atcgcgctct ttggtcacca
     2461 gacagtcatg acgacttaat tcctgtaatg attgtggctg cggatatttt tgcagatact
     2521 caggtgctgc gcataatatt cttttatttt tcgttaacag atgcgcaata taataatcag
     2581 gaatttcgtc attaatacga atatccagat caatattatc ctgcactaaa tcaatttgcc
     2641 gatcgaacaa ttcaaaatgc acctgtaact caggataatt gcgcataagt tcggtaatgg
     2701 ctggcgcaat atggctgcgc ccaaaaccaa aactacagcc aatacgaatc atcccctccg
     2761 ggcgcgtttt gatttgcgtg acgtcatcga ccagtcgctg atattgggtg aggatttcca
     2821 gcgcatgttc gtaacaacgt tggccacttt cagtcagcgc cacgccgcga gcggagcggt
     2881 tcagcagcgt tgttgccagc gtattttcaa ggatttggat ccgcttggtg acaaatgcag
     2941 gcgtctgccc aagcgtcgcc gctgcggcgc taaaactccc gctgtggacg atttctacca
     3001 gtacctgcaa gtctttggct aaaggccagc tattcagcat cgttaattat ccgcagttgt
     3061 gataagcgca gtgtatttcg caaaacattg ccattccctg ctctggcgca atattcattc
     3121 ttattttcgc gttgattcac gaactgttaa ttacatattc acggtggcaa aaaatataaa
     3181 accacatttt tagtggtagt ttgtggcggt gaatttttcc agacaaatac aaaaactgga
     3241 gttgccatga tgagcgaaag taataagcaa caggcagtga ataagttgac agagattgtc
     3301 gctaacttta ccgccatgat ttctacccga atgcctgatg acgtggtgga taaactaaaa
     3361 cagctaaagg atgccgaaac gtcgtcgatg gggaaaatta tctaccatac gatgttcgac
     3421 aacatgcaaa aagcgattga cctgaatcgt cctgcctgtc aggacaccgg ggagattatg
     3481 ttcttcgtta aagtcggttc ccgcttccca ctgcttggcg agctgcaaag catactcaaa
     3541 caagccgtgg aagaggcaac cgtcaaagcg ccactacgtc acaatgcggt agaaattttt
     3601 gacgaagtaa acaccggcaa aaataccggt agcggcgtac cgtgggtcac ctgggacatc
     3661 atccccgaca atgacgatgc ggaaatcgaa gtttacatgg caggcggcgg ctgcacgcta
     3721 cctggccgct cgaaagtgtt aatgccgtca gaaggctacg aaggcgtagt gaaattcgtc
     3781 ttcgaaaata tctccaccct cgccgtaaac gcctgtccac cggtactggt gggcgtgggc
     3841 atcgccacct cggtggaaac cgccgccgtg ctctcgcgaa aagccatttt gcgcccgatt
     3901 ggcagccgcc atcccaatcc aaaagcggca gagctggagc tacgcctgga agaaggactc
     3961 aaccgtctgg gaattggccc acaagggctg actggcaaca gttcagtgat gggcgtgcat
     4021 atcgaatctg ccgcccgcca tccgtcaacc atcggcgttg ctgtttctac aggctgctgg
     4081 gcgcatcgtc gcggcacact gctggttcat gccgatctca cctttgaaaa tctgtctcac
     4141 acccggagcg cgttatgaaa aagatcctga caaccccgat caaagctgaa gatctgcaag
     4201 atattcgcgt cggcgatgtg atctacctga ccggtacgct ggtgacctgc cgtgacgttt
     4261 gtcaccgccg cttgatcgaa ctgaaacgtc cgatccctta cgatctcaac ggcaaagcga
     4321 ttttccacgc tggccccatc gtgcgcaaaa acggcgataa atgggagatg gtttccgttg
     4381 gcccgacaac cagtatgcgt atggaaagtt ttgaacgtga atttatcgag cagaccggcg
     4441 tgaaactggt ggttggcaaa ggtggtatgg ggccgctgac cgaagaaggc tgccagaaat
     4501 tcaaggcgct acatgtgatt ttcccggcag gctgcgcggt gctggcggca acccaggtag
     4561 aagagattga agaagtgcac tggacagaac tcgggatgcc ggagtcactg tgggtctgcc
     4621 gggtcaaaga gttcggcccg ctgattgtct ctattgatac ccacggcaac aacctgatag
     4681 ccgaaaacaa aaagctgttc gccgaacgcc gcgatcccat cgtggaagag atctgcgagc
     4741 acgtccatta catcaaataa ccctcccgga gaggctcacc cctctccttt ttcgcaggca
     4801 taacacgatg aaaccttcca ctgaatggtg gcgatatctt gcgccgctgg cggtcatcgc
     4861 cattattgct ctacttccgg ttcccgcagg gctggagaac catacctggc tctactttgc
     4921 cgtttttact ggcgtgatcg ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc
     4981 gatggtgggt atctccatca tcgccatact ctctccctgg ctgctgttca gccccgaaca
     5041 gctcgcccag ccaggcttta aattcactgc aaaatccctc tcgtgggcgg tttccggttt
     5101 ttctaattcg gttatctggc tgattttcgc cgcctttatg tttggcacag gctatgaaaa
     5161 aaccgggctt ggacgccgca ttgcgctgtt tctggtgaaa aagatgggac atcgcacgct
     5221 gtttctcggc tatgcggtga tgttctccga gctgatcctc gcacctgtaa caccgtccaa
     5281 ctcggcgcgt ggtgcgggga ttatctatcc catcatccgt aacctgccac cgctctatca
     5341 atcacaacca aacgacagca gttcgcgcag cattggctcg tacatcatgt ggatggggat
     5401 tgttgccgac tgcgtgacca gcgccatttt cttgacggcg atggcaccaa acttgctgtt
     5461 aattggactg atgaaaagcg catctcacgc cacgctgagt tggggcgact ggttcctcgg
     5521 gatgttgccg ctcagtattt tgctggtgct gctggttccc tggctggctt atgtgctgta
     5581 cccgccggta ctgaagtctg gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc
     5641 aatgggcccg ctctgttcgc gtgaaaaacg gatgctgggg ctgatggtag gcgcgctggt
     5701 gctgtggatt ttcggcggtg attatatcga tgccgcgatg gtcggttaca gcgtggtggc
     5761 actgatgctg cttctgcgca ttatcagttg ggacgacatt gtcagtaata aagcggcgtg
     5821 gaacgttttc ttctggctgg cctcgcttat caccctcgct accggactca acaacactgg
     5881 ttttattagc tggtttggca aactgttagc aggcagctta agcggttatt cgccaacgat
     5941 ggtgatggtg gcgttgattg tggtgtttta tctgctgcgc tactttttcg ccagcgccac
     6001 ggcgtatacc tccgccctcg caccgatgat gattgccgct gcgctggcga tgccggaaat
     6061 cccgctgccg gtgttctgcc tgatggttgg cgcggcaatt ggtctgggga gcattcttac
     6121 accatacgcc accggaccca gtccgattta ctacggtagt ggttatctgc caacggtgga
     6181 ttactggcga ctgggggcga tttttggact gatattcctc gtgttgctgg tgattaccgg
     6241 cttgctgtgg atgcccgtgg tgttgcttta agtgaggacg gtgccggaaa ttgctctccg
     6301 gcaccggata gttttacgca gccggtaact ctgccagtgg ccagcgcgga cgcacgctaa
     6361 cgccgagatc cgccgtcgcg cctgctttaa accgcaccat tccggcatag gcgatcatcg
     6421 cgccgttatc agtacaaaat tccggacgcg cgtagaacac ttcgccgcgg cgttttttca
     6481 tcatttccgc cagcttcgcc cgtaacgtgc ggttagcact cacgccgccc gccatgacca
     6541 gtcgcttaaa gcccgtctga tccaacgctc gcttacactt aatcatcaac gtatcgacca
     6601 ccgcatcttc aaaggcgcgg gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac
     6661 gaatcgtgtt cgccgcaaag gttttcagac cagaaaagct gaaatccagc cccggacggt
     6721 cggtcatcgg acgcgggaaa acaaagcgcc cggcagtacc ctgagccgcc attttcgaca
     6781 gtaacggtcc gccaggataa tccagcccca gcagcttcgc ggttttatca aacgcttcac
     6841 cggcggcatc atcgatagac tcgccgagca gctcgtactg accaatgcca gtcacgctga
     6901 ttaactgcgt atgaccgccg gacaccagca gcgcgacaaa cggaaattcc ggcgggttat
     6961 cttccagcat cggcgctaac agatgccctt ccatatggtg taccgggatt gccggaacgt
     7021 tccaggcaaa cgccagagaa cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc
     7081 cagggcctgc ggtataggcc acagcatcaa tgtcttttgc cgttaaacca gactccttta
     7141 gcgccgcctg gatcaacggt acggttttgc gcacgtgatc gcgggaggcc agttcaggca
     7201 cgacgccgcc gtagtcagcg tgcaatttca cctgactata caattggttg gctaacaaac
     7261 ctttttcatc gtcgtaaatg gcgatgccgg tttcatcgca ggaagtttca atacccagta
     7321 cacgcatgac ttgttttacc tcgctttatt accgcgcagt gtaggaccaa tgcgggttga
     7381 tgtaaaactt tgttcgcccc tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt
     7441 ccctttcaaa aaaggccgcg gtgctttaca aagcagcagc aattgcagta aaattccgca
     7501 ccattttgaa ataagctggc gttgatgcca gcggcaaacc gaattaatca aaggtgagag
     7561 gcacatgccg gtaattaaag tacgtgaaaa cgagccgttc gacgtagctc tgcgtcgctt
     7621 caagcgttcc tgcgaaaaag caggtgttct ggcggaagtt cgtcgtcgtg agttctatga
     7681 aaaaccgact accgaacgta agcgcgctaa agcttctgca gtgaaacgtc acgcgaagaa
     7741 actggctcgc gaaaacgcac gccgcactcg tctgtactaa ttccccgaga gcgttgctct
     7801 ccgatcagac cgagttgtag ttgtaaggcc gtgcttccga aaggaatgcg cggcttattt
     7861 tcgtttatga attgctaaaa atcggggcct atggctggac gaatcccacg cgtattcatt
     7921 aatgatctgc tggcacgcac tgacatcgtc gatctgatcg atgcccgtgt gaagctgaaa
     7981 aagcagggca agaatttcca cgcgtgttgt ccattccaca acgagaaaac cccgtcattc
     8041 accgttaacg gtgagaaaca gttttaccac tgctttggat gtggcgcgca cggcaacgcg
     8101 atcgacttcc tgatgaacta cgacaagctc gagttcgttg aaacggtcga agagctggca
     8161 gcaatgcaca atcttgaagt gccatttgaa gcaggcagcg gccccagcca gatcgagcgc
     8221 catcagaggc aaacgcttta tcagttgatg gacggtctga atacgtttta ccaacaatct
     8281 ttacaacaac ctgttgccac gtctgcgcgc cagtatctgg aaaaacgcgg attaagccac
     8341 gaggttatcg ctcgctttgc gattggtttt gcgccccccg gctgggacaa cgtcctgaag
     8401 cggtttggcg gcaatccaga aaatcgccag tcattgattg atgcggggat gttggtcact
     8461 aacgatcagg gacgcagtta cgatcgtttc cgcgagcggg tgatgttccc cattcgcgat
     8521 aaacgcggtc gggtgattgg ttttggcggg cgcgtgctgg gcaacgatac ccccaaatac
     8581 ctgaactcgc cggaaacaga cattttccat aaaggccgcc agctttacgg tctttatgaa
     8641 gcgcagcagg ataacgctga acccaatcgt ctgcttgtgg tcgaaggcta tatggacgtg
     8701 gtggcgctgg cgcaatacgg cattaattac gccgttgcgt cgttaggtac gtcaaccacc
     8761 gccgatcaca tacaactgtt gttccgcgcg accaacaatg tcatttgctg ttatgacggc
     8821 gaccgtgcag gccgcgatgc cgcatggcga gcgctggaaa cggcgctgcc ttacatgaca
     8881 gacggccgtc agctacgctt tatgtttttg ccggatggcg aagaccctga cacgctggta
     8941 cgaaaagaag gtaaagaagc gtttgaagcg cggatggagc aggcgatgcc gctttccgca
     9001 tttctgttta acagcctgat gccgcaagtt gatctgagta cccctgacgg gcgcgcacgt
     9061 ttgagtacgc tggcactgcc attgatatcg caagtgccgg gcgaaacgct gcgaatatat
     9121 cttcgtcagg aattaggcaa caaattaggc atacttgatg acagccagct tgaacgatta
     9181 atgccaaaag cggcagagag cggcgtttct cgccctgttc cgcagctaaa acgcacgacc
     9241 atgcgtatac ttatagggtt gctggtgcaa aaaccagaat tagcgacgtt ggtcccgccg
     9301 cttgagaatc tggatgaaaa taagctccct ggacttggct tattcagaga actggtcaac
     9361 acttgtctct cccagccagg tctgaccacc gggcaacttt tagagcacta tcgtggtaca
     9421 aataatgctg ccacccttga aaaactgtcg atgtgggacg atatagcaga taagaatatt
     9481 gctgagcaaa ccttcaccga ctcactcaac catatgtttg attcgctgct tgaactgcgc
     9541 caggaagagt taatcgctcg tgagcgcacg catggtttaa gcaacgaaga acgcctggag
     9601 ctctggacat taaaccagga gctggcgaaa aagtgattta acggcttaag tgccgaagag
     9661 cgatcgggaa gcccccgaca gccgcactga gaggcagcgg caaatatata agtacgccct
     9721 cgtaattatc gttggcggta aacaaccgtt ggatttcagc gttaacggct gaaggacatc
     9781 gggtcaatcg cccaacacca acctcatgaa ataagtgtgg ataccgtctt atggagcaaa
     9841 acccgcagtc acagctgaaa cttcttgtca cccgtggtaa ggagcaaggc tatctgacct
     9901 atgccgaggt caatgaccat ctgccggaag atatcgtcga ttcagatcag atcgaagaca
     9961 tcatccaaat gatcaacgac atgggcattc aggtgatgga agaagcaccg gatgccgatg
    10021 atctgatgct ggctgaaaac accgcggacg aagatgctgc cgaagccgcc gcgcaggtgc
    10081 tttccagcgt ggaatctgaa atcgggcgca cgactgaccc ggtacgcatg tacatgcgtg
    10141 aaatgggcac cgttgaactg ttgacccgcg aaggcgaaat tgacatcgct aagcgtattg
    10201 aagacgggat caaccaagtt caatgctccg ttgctgaata tccggaagcg atcacctatc
    10261 tgctggaaca gtacgatcgt gttgaagcag aagaagcgcg tctgtccgat ctgatcaccg
    10321 gctttgttga cccgaacgca gaagaagatc tggcacctac cgccactcac gtcggttctg
    10381 agctttccca ggaagatctg gacgatgacg aagatgaaga cgaagaagat ggcgatgacg
    10441 acagcgccga tgatgacaac agcatcgacc cggaactggc tcgcgaaaaa tttgcggaac
    10501 tacgcgctca gtacgttgta acgcgtgaca ccatcaaagc gaaaggtcgc agtaacgctg
    10561 ccgctcagga agagatcctg aaactgtctg aagtattcaa acagttccgc ctggtgccga
    10621 agcagtttga ctacctggtc aacagcatgc gcgtcatgat ggaccgcgtt cgtacgcaag
    10681 aacgtctgat catgaagctc tgcgttgagc agtgcaaaat gccgaagaaa aacttcatta
    10741 ccctgtttac cggcaatgaa accagcgata cctggttcaa cgcggcaatt gcgatgaaca
    10801 agccgtggtc ggaaaaactg cacgatgtct ctgaagaagt gcatcgcgcc ctgcagaaac
    10861 tgcagcagat tgaagaagaa accggcctga ccatcgagca ggttaaagat atcaaccgtc
    10921 gtatgtccat cggtgaagcg aaagcccgcc gtgcgaagaa agagatggtt gaagcgaact
    10981 tacgtctggt tatttctatc gccaagaaat acaccaaccg tggcttgcag ttccttgacc
    11041 tgattcagga aggcaacatc ggtctgatga aagcggttga taaattcgaa taccgccgtg
    11101 gttacaagtt ctccacctac gcaacctggt ggatccgtca ggcgatcacc cgttctatcg
    11161 cggatcaggc gcgcaccatc cgtattccgg tgcatatgat tgagactatc aacaaactca
    11221 accgtatttc tcgccagatg ctgcaagaga tgggccgtga accgacgccg gaagaactgg
    11281 ctgaacgtat gctgatgccg gaagacaaga tccgcaaagt gctgaagatc gccaaagagc
    11341 caatctccat ggaaacgccg atcggtgatg atgaagattc gcatctgggg gatttcatcg
    11401 aggataccac cctcgagctg ccgctggatt ctgcgaccac cgaaagcctg cgtgcggcaa
    11461 cgcacgacgt gctggctggc ctgaccgcgc gtgaagcgaa agttctgcgt atgcgtttcg
    11521 gtatcgatat gaacaccgac cacacgctgg aagaagtggg taaacagttc gacgttaccc
    11581 gcgaacgtat ccgtcagatc gaagcgaagg cgctgcgcaa actgcgtcac ccgagccgtt
    11641 ctgaagtgtt gcgtagcttc ctggacgatt aatcggtagg ccggatcagg cgttacgccg
    11701 cacccggcac tagtccctct gcacaaacgc caccttttcg gtggcgtttt tatcgcccac
    11761 gcactaccag cgcctggtcc agctcgcgat atgcttcaac cagtttctct agtgaaacgc
    11821 gacttaaacc gctgggattt ggcagcaccc aaatctgcgt cgaaccaatg gtgagcgttt
    11881 gtttccccca ctgtgcaccg cgctggctga atccctgttc atatgcttgt ttgcccagaa
    11941 tcgccaacgc ctgcggctga taatcttcaa ttttttcaat cagcttacgc ccacctgcgt
    12001 gcagctcctg ctttgaaact tcattggctt gcaccgttgg acggtctacc agtttggtga
    12061 cgccacaacg ataatccagc agatgctgtg cctcctgcgg cttcaactga cggtcggtaa
    12121 acccggcctg atatatcacc ttccagaagc gatttgctgg atgagcaaag ggaaaaccag
    12181 tcccggcgga tgaaagccca gggttgatac cgcaaaacac gacccgtaac cctggagcca
    12241 aaatatcctc aaccatattt actcctttct aacatcacca ggaaaagtat aacggattga
    12301 aaatacattg tttataaaaa cagcaggcgc acggtaatgg ctggattgcg acacggagtt
    12361 actttataat ccgctaccat ggccccttag ctcagtggtt agagcaggcg actcataatc
    12421 gcttggtcgc tggttcaagt ccagcagggg ccaccagata tagcaaaggc tgacgagaaa
    12481 tcgtcagcct ttttcttttt atatatcagt tactttgcgt gccagtaagc cgctgcacgt
    12541 acccgctgtg ggtcatactg ttccgcttca aagcggcggc ttaaattctt aacgacttta
    12601 ccttcgccgg ttatccagat gaagtaatca tcggcaggga tttgcatctg cgccagacgc
    12661 gcatctaccg cctgctcatc atgtgccagc cattcgatat taaaaccatc aaggtgcgcg
    12721 agataatcct gacaggcgtt atcccgcacg ctaaccagcg cactaacttg cggtttaacg
    12781 gcaagtttgc tcaacgtttc caggcggcgg cgcagtgcgg gcattccgga ttcatcgcag
    12841 acatacagct gatacgcgta atcttccggc accaccagcg aaccgcgcgg acctgccacc
    12901 gtaagtttat cgcccggttg cgcctgcatc gcccagccgc tggcgacccc accgtcgtga
    12961 ataaagaaat cgatcgccag ttcatggcgt agttcgtcat acagcggcgt atagtcacgc
    13021 gacggtgggc gtggtccttc cggccagacg atgccctctt ccgttaccgt tggcggcaca
    13081 aagtgagcgt caggttgagg aaagaagagt ttgctgtgat cgtcaaagcc gcgcgatgta
    13141 aaaccgtcca gcgcctcgcc gccgaggaca atgcgctgaa aaccggcgct gatgcgctca
    13201 acgcgtaaca cagtcagttc acggaagcgc agatcattgc gaacgcgctg cgggtagcgg
    13261 ggggagttat tcattgttat cgccttcgtg atagcaatca gatatatcta aataattttc
    13321 gtaaatgata atgattgtta atcatgataa atgcaagcga tttgtagaac tgatatatct
    13381 gaagtatgtg aaggcattca gtgccatagc aatgctgccg gatgcggcgt gaacgtctta
    13441 tccggcctgg atgtgaggtg cttccacgtt taaacatcat ttttgtactt gcagtaatgc
    13501 tcacatcaga tataaattag atatatctaa tcaagcaaaa ggaggctgat atgagccatc
    13561 atcacgaagg gtgttgtaaa catgaaggcc agccacgcca tgagggctgc tgcaaaggtg
    13621 agaagtcaga acacgagcac tgcggacacg gtcaccagca tgaacacggt caatgctgcg
    13681 gtggtcgcca cggtcgcggc ggcggtcgtc ggcaacgttt ctttggtcac ggtgaattgc
    13741 gtctggtgat tctggatatt ctctcgcgcg atgacagcca cggttacgaa ttgattaaag
    13801 cgattgagaa cctaacccag gggaattaca ccccaagccc gggcgtcatc tacccaacgc
    13861 tggattttct gcaggagcag tcgctgatta ccatccgcga agaggaaggt ggtaagaagc
    13921 agattgcgct gaccgaacaa ggcgcgcagt ggctggaaga aaaccgcgaa caggtggaga
    13981 tgattgaaga acgcatcaaa gcgcgttgcg ttggcgcggc gctgcgccag aacccgcaaa
    14041 tgaagcgggc gctggataat tttaaagcgg tgctggattt acacgtcaac cagagcgata
    14101 tcagtgatgc acaaataaaa aagatcattg cggtgatcga ccgcgccgct tttgatatta
    14161 cgcaactgga ttaatcgccg catccgccag tggcgcggtg caattgccgg atgcgacgct
    14221 tgacgcgcct tatccggcct acacccgcta cacaccccgc aggcctgata agatgcgcca
    14281 gcatcgcatc aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc
    14341 gtcaccgcgt cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc
    14401 tcttccacca gctcggcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc
    14461 aggctggaaa gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg
    14521 gtgacgtttt tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc
    14581 tgcgaaccgg attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca
    14641 gcattagcgc tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg
    14701 ccctgttcgc cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga
    14761 aacgcaatat cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata
    14821 tcgtccattg tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg
    14881 gcggtgatag aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc
    14941 gccatttggt tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt
    15001 tcgttcagtt catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc
    15061 tggcttgaga catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt
    15121 aatgtcagcc ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc
    15181 gccaccttca gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa
    15241 caagcgcttg ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca
    15301 acaaaccaaa gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc
    15361 cagcgaagcg gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta
    15421 tgaatacgct tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca
    15481 atctcttcat ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc
    15541 accatcggta ccgcattggc ccgcacccaa taatggtcac catttttgcg gcgatttttc
    15601 acgatgccgc tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct
    15661 tttggcatat ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta
    15721 aagccgctca cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta
    15781 gtggacatca gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga
    15841 gaagacatgt tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg
    15901 tcggccccgc tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta
    15961 aagcacaaat ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt
    16021 aattatcttg cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat
    16081 catcaaatcc atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat
    16141 taaatattgc acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg
    16201 gagtggcgca atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc
    16261 atattttgaa caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga
    16321 atctcattga gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga
    16381 ttgaatactt caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg
    16441 ccggcgggga ttacggagcc gtagagtggc aagcgggagg tttaaatacg cttgtcgaca
    16501 cccagggaca ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc
    16561 gtaatccagt tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc
    16621 aggagctgct cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg
    16681 gtaaactgaa atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga
    16741 agctggcgaa agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg
    16801 cgttccacgg taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac
    16861 cgtttatgcc actgctgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc
    16921 gcacggctct taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac
    16981 cgattcaggg tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta
    17041 agctatgcga tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc
    17101 gcacgggcaa gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg
    17161 ccaaagcgct cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt
    17221 tttcagttct gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg
    17281 cctgtgcggc ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg
    17341 ctgagcaaaa aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg
    17401 atctggtaca ggaagcgcgt ggtaaaggga tgttgatggc aattgagttt gttgataacg
    17461 aaatcggcta taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc
    17521 tcaataacgc caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg
    17581 aactggtgat caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag
    17641 cgtaatatca aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta
    17701 cgcccgcagg catcatccgc tccggcgtta acaacacgct ttcactgccg tcatcggttt
    17761 ccgcacacaa cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc
    17821 ataagaccac caccgttttc cccatcagct cttcttcgct gtagtacggc accaggctgg
    17881 tcacggtttg cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt
    17941 tttcatggcg tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag
    18001 cgtaagccac ggtttccatt gtttcctccc ttcgggtaaa attttactaa actatagcaa
    18061 aagtttttct caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtgag
    18121 ccgctgtgtt accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc
    18181 cgataagcac ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg
    18241 gcaacactaa aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc
    18301 ttaaatgacg atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc
    18361 gccgaaaagc tggagtacaa gaccagtagt gcccgtaaac tccagacagg tgcagtcaac
    18421 caacaccata ttctggctat ctacagctac caacaggagc tggagatcaa cgatccttac
    18481 tatctggcga tccgccacgg cattgaaact cagtgcgaaa agctgggcat tgagctcacc
    18541 aactgttatg aacacagcgg attaccagac attaaaaacg tcaccggtat tttaattgtc
    18601 ggcaaaccca cgcccgccct gcgcgccgcc gcctgtgcgt tgaccgacaa tatctgtttt
    18661 atcgactttc acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc
    18721 agtaaagaaa tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc
    18781 ggtgaagatg agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga
    18841 ctgaaacaag tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt
    18901 tatgaactgg caaaacaaat gctggcgcga gaagactatc cgaaggcact gtttgttgct
    18961 tccgattcca ttgctatcgg cgtactgcgg gcaattcatg aacgaggcct gaacatccca
    19021 caggatattt cgcttatcag cgttaacgat atccccaccg cgcgatttac ctttccaccg
    19081 ctctccaccg tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat
    19141 gaaaaagccc gcgatggtcg cgcgctgccg ctgttagtct tcgttcccag caaattaaaa
    19201 ctgcgcggca cgacccgtta aatcccctta cacactgtcc ggcaatcgtt tttgccggac
    19261 agtgctgccg tttattttcg tgatccagtt aaagtaaatg catttacctg ctacttttta
    19321 gtaaaaattt tactaaactc cccagcaatt acacaaacta ccatcaccat gaatggttcc
    19381 gatttctctc taccgggagg ccctatgaat cgctgggaaa acattcagct cacccacgaa
    19441 aaccgacttg cgccgcgtgc gtactttttt tcatatgatt ctgttgcgca agcgcgtacc
    19501 tttgcccgcg aaaccagcag cctgtttctg cccttaagcg gtcagtggaa tttccacttt
    19561 tttgaccatc cgctgcaagt accagaagcc ttcacctctg agttaatggc tgactggggg
    19621 catattaccg tccccgccat gtggcaaatg gaaggtcacg gcaaactgca atataccgac
    19681 gaaggttttc cgttccccat cgatgtgccg tttgtcccca gcgataaccc aaccggtgcc
    19741 tatcaacgta ttttcaccct cagcgacggc tggcagggta aacagacgct gattaaattt
    19801 gacggcgtcg aaacctattt tgaagtctat gttaacggtc agtatgtggg tttcagcaag
    19861 ggcagtcgcc tgaccgcaga gtttgacatc agcgcgatgg ttaaaaccgg cgacaacctg
    19921 ttgtgtgtgc gcgtgatgca gtgggcggac tctacctacg tggaagacca ggatatgtgg
    19981 tggtcagcgg ggatcttccg cgatgtttat ctggtcggaa aacacctaac gcatattaac
    20041 gatttcactg tgcgtaccga ctttgacgaa gcctattgcg atgccacgct ttcctgcgaa
    20101 gtggtgctgg aaaatctcgc cgcctcccct gtcgtcacga cgctggaata taccctgttt
    20161 gatggcgaac gcgtggtgca cagcagcgcc attgatcatt tggcaattga aaaactgacc
    20221 agcgccagct ttgcttttac tgtcgaacag ccgcagcaat ggtcagcaga atccccttat
    20281 ctttaccatc tggtcatgac gctgaaagac gccaacggca acgttctgga agtggtgcca
    20341 caacgcgttg gcttccgtga tatcaaagtg cgcgacggtc tgttctggat caataaccgt
    20401 tatgtgatgc tgcacggcgt caaccgtcac gacaacgatc atcgcaaagg ccgcgccgtt
    20461 ggaatggatc gcgtcgagaa agatctccag ttgatgaagc agcacaatat caactccgtg
    20521 cgtaccgctc actacccgaa cgatccgcgt ttttacgaac tgtgtgatat ctacggcttg
    20581 tttgtgatgg cggaaaccga cgtcgaatcg cacggctttg ctaatgtcgg cgatatcagc
    20641 cgtattaccg acgatccgca gtgggaaaaa gtctacgtcg agcgcattgt tcgccatatt
    20701 cacgcgcaga aaaaccatcc gtcgatcatc atctggtcgc tgggcaatga atccggctat
    20761 ggctgtaaca tccgcgcgat gtaccacgca gcgaaggcgc tggatgacac gcgactggtg
    20821 cattacgaag aagatcgcga tgctgaagtg gtcgatatta tttccaccat gtacacccgc
    20881 gtgccgctga tgaatgagtt tggtgaatac ccgcatccga agccgcgcat catctgtgaa
    20941 tatgctcatg cgatggggaa cggaccaggc gggctgacgg agtaccagaa cgtcttctat
    21001 aagcacgatt gcattcaggg acattatgtc tgggaatggt gcgaccacgg gatccaggcg
    21061 caggatgaca acggcaacgt ctggtataaa ttcggcggcg actacggcga ctatcccaac
    21121 aactataact tctgtcttga tggtttgatc tattccgatc agacgccggg accgggcctg
    21181 aaagagtaca aacaggttat cgcgccggta aaaatccacg cgctggatct gactcacggc
    21241 gagctgaaag tcgaaaataa actgtggttt accacgcttg atgactacac cctgcacgca
    21301 gaggtgcgcg tcgaaggtga aacgctcgcg acgcagcaga ttaaactgcg cgacgttgcg
    21361 ccgaacagcg aagccccctt gcagatcacg ctgccgcagc tggacgcccg cgaagcgttc
    21421 ctcaacatta cggtgaccaa agattcccgc acccgctaca gcgaagccgg gcattctatc
    21481 gccacttatc agttcccgct gaaggaaaac accgcgcaac cagtgccttt cgcaccaaat
    21541 aatgcgcgtc cgctgacgct ggaagacgat cgtttgagct gcaccgttcg cggctacaac
    21601 ttcgcgatca ccttctcaaa aatgagtggc aaaccgacat cctggcaggt gaatggcgag
    21661 tcgctgctga cccgcgagcc aaagatcaac ttcttcaagc caatgatcga caaccacaag
    21721 caggagtacg aagggctgtg gcaaccgaat catttgcaga tcatgcagga acatctgcgc
    21781 gactttgccg tagaacagag cgatggtgaa gtgttgatca tcagccgcac agttattgcc
    21841 ccgccggtgt ttgacttcgg gatgcgctgc acctacatct ggcgcatcgc tgccgatggc
    21901 caggttaacg tggcgctttc cggcgagcgt tacggcgact atccgcacat cattccgtgc
    21961 atcggtttca ccatggggat taacggcgaa tacgatcagg tggcgtatta cggtcgtgga
    22021 ccgggcgaaa actacgccga cagccagcag gctaacatca tcgatatctg gcgcagcacc
    22081 gtcgatgcca tgttcgagaa ctatcccttc ccgcagaaca acggcaaccg tcagcatgtc
    22141 cgctggacgg cactgactaa ccgccacggc aacggtctgc tggtggttcc gcagcgccca
    22201 attaacttca gcgcctggcg ctatacccag gaaaacatcc acgctgccca gcactgtaac
    22261 gagctgcagc gcagtgatga catcaccctg aacctcgacc accagctgct tggcctcggc
    22321 tccaactcct ggggcagcga ggtgctggac tcctggcgcg tctggttccg tgacttcagc
    22381 tacggcttta cgttgctgcc ggtttctggc ggagaagcta ccgcgcaaag cctggcgtcg
    22441 tatgagttcg gcgcagggtt cttttccacg aatttgcaca gcgagaataa gcaatgagga
    22501 tcatcgataa cttagaacag ttccgccaga tttacgcctc tggcaagaag tggcaacgct
    22561 gcgttgaagc gattgaaaat atcgacaaca ttcagcctgg cgtcgcccac tccatcggtg
    22621 actcattgac ttaccgcgtg gagacagact ccgcgaccga tgcgctattt accgggcatc
    22681 gacgctattt tgaagtgcat tactacctgc aagggcagca aaaaattgaa tatgcgccga
    22741 aagagacatt acaggtagtg gaatattatc gtgatgaaac tgaccgtgaa tatttaaaag
    22801 gctgcggaga aaccgttgag gtccacgaag ggcaaatcgt tatttgcgat atccatgaag
    22861 cgtatcggtt tatctgcaat aacgcggtca aaaaagtggt tctcaaagtc accatcgaag
    22921 atggttattt ccataacaaa taacaactac ggcggcaaaa ggagtttgcc gccaccgcta
    22981 ccctacacat tttcggagat gtgttatgtc tgataccaaa cgtaatacaa tcggcaaatt
    23041 cggcttgctc tcgctgactt ttgccgccgt ttacagcttt aacaacgtta tcaataataa
    23101 tattgagctt ggactggcct cggcaccgat gtttttcctc gcgacgattt tttattttat
    23161 tcccttctgt ctgatcatcg cagaatttgt ttcgttaaat aaaaactcag aagccggtgt
    23221 ctacgcgtgg gtaaaaagtt cgctgggcgg acgttgggca tttattactg cctataccta
    23281 ctggttcgta aacctgttct ttttcacctc actgttgccg cgcgttattg cttatgcttc
    23341 gtatgccttc ctcggatacg aatatattat gacgccggtt gccaccacca ttatcagtat
    23401 ggtgctgttc gccttctcca cctgggtttc caccaacggg gcgaaaatgt tggggccaat
    23461 tacctccgtc acttcaacgc tgatgctgct gttaacgctc tcctacattt tactggcagg
    23521 tacggcgctg gttggcggcg tacagcctgc tgaccccatc accgttgacg cgatgatccc
    23581 gaacttcaac tgggcgttcc tcggcgttac cacctggatc tttatggccg caggtggcgc
    23641 ggagtccgtc gctgtgtacg ttaacgacgt caaaggcggt tcgaaatcgt tcgttaaagt
    23701 gatcatcctc gccgggattt ttatcggcgt actgtattcc gtctcctcgg tgctgattaa
    23761 cgtcttcgtc agcagcaaag agttgaaatt taccggcgga tcggtgcagg tattccacgg
    23821 catggcggcg tattttggtc taccggaagc gttgatgaat cgctttgtcg gtctggtgtc
    23881 ctttaccgcg atgttcggtt ccctgctgat gtggaccgca acgccggtga aaattttctt
    23941 ctccgaaatc ccggaaggca tctttggtaa gaaaaccgtc gaactgaacg aaaacggcgt
    24001 tccggcgcgc gcagcgtgga tccagttcct gatcgtcatc ccgctgatga ttatcccgat
    24061 gctcggttcc aataccgtgc aggatctgat gaatactatt attaatatga ccgccgcagc
    24121 gtccatgctt ccgccgttat tcatcatgct ggcttacctg aatttacgcg ccaaattaga
    24181 tcacctgcca cgcgatttcc gtatgggctc ccgccgcacc ggtattatcg ttgtttcaat
    24241 gctgattgcg atatttgccg tagggtttgt cgcttcgaca ttcccgactg gcgcgaatat
    24301 tctgaccatc attttttata acgtcggcgg tattgttatc ttccttggct ttgcgtggtg
    24361 gaaatacagt aaatatataa agggattaac ggctgaagag cgccatattg aagcgacgcc
    24421 agccagcaat gttgattaag cagaataata aaaaatgagc aggcattttc cctcttcctg
    24481 gatgaggaga gggaaaatag tttctgcctt tatattttta taattacaac gataaaaggc
    24541 tgtacttttt ctttagctca tggattaaca caatgaaatt aatcactgca ccatgcagag
    24601 cattacttgc tctgccgttt tgctacgcct tttctgcggc aggagaagaa gcacgtccgg
    24661 cagaacatga cgacacaaaa acacctgcaa ttacctcgac atcttctcct tcatttcgtt
    24721 tttacggcga attaggggtt ggtggatata tggatttaga gggtgagaat aaacataaat
    24781 acagcgacgg tacctatatt gaaggtggcc tggagatgaa gtacggctcc tggttcggcc
    24841 tgatttacgg cgaaggctgg accgtgcagg ccgaccacga cggcaatgcc tgggtgccag
    24901 accatagctg gggtggtttc gagggcggaa ttaaccgttt ctatggcggt tatcgtacca
    24961 atgatggcac cgaaatcatg ctcagtctgc gtcaggattc ctcgctggat gacctgcaat
    25021 ggtggggcga cttcaccccc gatctgggct acgtcattcc caatacccgc gacattatga
    25081 ccgcgctgaa ggtgcagaac ttaagcggca actttcgtta tagcgtcacc gccacacctg
    25141 ccggacatca tgacgaaagc aaagcctggc tacattttgg caagtacgat cgctatgacg
    25201 acaaatacac ctatccggca atgatgaacg gttacatcca gtatgacctt gccgaaggca
    25261 tcacctggat gaacggtctg gaaatcaccg acggcacagg acagctctat ctcacgggcc
    25321 tgctaactcc taactttgcc gctcgcgcct ggcaccatac cggacgcgcc gacgggctgg
    25381 acgtaccggg aagtgaaagt gggatgatgg tgagcgccat gtatgaagcg ttaaagggcg
    25441 tttatctctc caccgcttac acctacgcca aacatcgccc tgaccacgct gacgatgaaa
    25501 ccacctcttt catgcagttt ggtatctggt acgaatacgg cggcggacgt ttcgccacgg
    25561 cttttgatag ccgcttctac atgaaaaatg cctctcacga tcccagcgac caaatcttcc
    25621 tgatgcaata tttctactgg taataaggac tgtaatttca tgaaaataaa aactatttta
    25681 acgccagtaa cctgcgctct gctgataagt ttttccgccc atgccgctaa cgccgacaat
    25741 tataaaaacg tgattaaccg tactggcgcg ccgcagtaca tgaaggatta cgattacgac
    25801 gatcaccagc gttttaatcc gtttttcgat ctcggagcct ggcatggtca tctgttgcca
    25861 gacggcccta acaccatggg cggctttccg ggcgttgcgc tgctgacgga agagtacatc
    25921 aactttatgg ccagcaattt cgaccgcctg accgtctggc aggacggcaa gaaagtcgac
    25981 tttacgctgg aggcatacag tattcccggt gcgctggtgc aaaaactgac agcaaaagat
    26041 gtgcaagtcg aaatgactct gcgcttcgcc acgccgcgca cgtcactact ggaaaccaaa
    26101 atcaccagca ataaaccgct ggatctggtg tgggacggcg aactgctgga aaaactggaa
    26161 gcgaaagaag ggaaaccgct ttccgataaa accattgctg gcgaataccc tgactatcag
    26221 cgcaaaatca gcgccacccg tgatggcctg aaagtcacct ttggcaaagt gcgcgccacc
    26281 tgggatctgc tgacctccgg cgaatcggaa tatcaggtgc ataaatccct gccggtgcag
    26341 actgaaatca acggtaatcg ctttaccagt aaggcgcata tcaacggttc gaccacgctc
    26401 tacaccacct attcccatct gctgaccgct caggaagtta gcaaagagca aatgcagatc
    26461 cgcgatattc tggcgcgtcc ggcgttttat ctcaccgcct cgcagcaacg ctgggaagaa
    26521 tacctgaaga aagggttaac caatccggat gcgacgccgg aacagacgcg cgtcgcggtg
    26581 aaagcgatcg aaacgctcaa cggtaactgg cgctcaccgg gcggtgcggt gaaatttaac
    26641 accgttacac cgtcggtgac cgggcgctgg ttctccggca atcagacctg gccgtgggat
    26701 acctggaagc aggcgtttgc gatggcgcat ttcaatccgg acatcgccaa agagaatatc
    26761 cgcgcggtct tctcctggca gatccagcct ggcgatagcg tgcgtccgca ggatgtgggc
    26821 tttgtccccg acctgatagc gtggaacctt agccccgagc gtggtggcga tggcggcaac
    26881 tggaacgaac gtaataccaa acccagcctt gccgcctggt cggtgatgga agtgtacaac
    26941 gtcacccagg ataaagcctg gttggcagag atgtacccga aactggtggc ctatcacgac
    27001 tggtggttac gtaaccgcga tcacaacggc aacggcgtgc cggaatatgg cgcgacccgc
    27061 gacaaagccc acaacactga gagcggcgag atgctgttta cggtgaagaa aggcaacaaa
    27121 gaagagacgc agtctgggct gaacaactac gcccgcgtgg tggagaaagg ccagtacgac
    27181 agtctggaaa ttccggcaca ggtcgctgcg tcgtgggaat cggggcgtga tgatgccgcc
    27241 gtctttggtt ttatcgacaa agaacagttg gataaatatg tcgctaatgg cggcaaacgt
    27301 agcgactgga cggtgaaatt cgccgaaaac cgcagtcagg acggaacgtt gctgggctac
    27361 tcgctattgc aggagtcggt ggatcaggcc agctatatgt acagcgataa ccattatctg
    27421 gcggagatgg caaccatcct cggtaagcca gaagaggcca aacgctatcg ccagttggca
    27481 cagcagctcg cggactacat caacacctgt atgttcgacc cgactaccca gttctactat
    27541 gacgtacgta ttgaagataa accactggcg aacggctgcg cgggcaaacc gattgttgag
    27601 cgcggtaaag ggccggaagg ctggtcgccg ctgtttaacg gtgcggcaac gcaggccaat
    27661 gccgacgcgg tggtgaaggt gatgctcgat cctaaagagt tcaacacctt tgtcccgctg
    27721 ggaacggcgg cgttaaccaa tccggctttt ggcgctgata tctactggcg tgggcgcgta
    27781 tgggtggatc agttctggtt tggtctgaaa gggatggagc gttacggtta tcgcgatgat
    27841 gccctgaaac tggcggatac gttcttccgt cacgccaaag gattaaccgc cgatggccca
    27901 attcaggaga attacaaccc gctgactggc gcacagcaag gcgcaccaaa tttctcctgg
    27961 agtgccgcgc atttgtatat gttgtataac gattttttcc gtaagcaata atttgcaact
    28021 gctggcggat gcggcgtaaa cgccttatcc gccctacatg tgcaatcctc ctggccggat
    28081 aaaacgcgac aagcgtcgca tccggcatct gcaccgtgcc actggcggat gcggcgtgaa
    28141 cgccttatcc gccctacatg tgcaatcccc caggccggat aaaacgcggc aagcgtcgca
    28201 tccggcgtta ccaccgggcg tattcttttt gaatcccatc acaaaccccg cactcccctt
    28261 ttcccttttc tccggcgacg gctaaattag aactcatccg accacataac aattatttta
    28321 catactggac aattttatga gctacccgtc gctgttcgcc ccgctggatt taggttttac
    28381 cacgttaaaa aaccgcgtgt tgatgggctc aatgcacacc gggctggagg aatacccgga
    28441 cggtgccgag cggctggcag cgttttatgc cgaacgcgcc cgtcacggcg tggcgctgat
    28501 tgtcagcggc ggcattgcac cagatttaac aggcgttggc atggaaggtg gcgcaatgct
    28561 caacgacgcc agccagatcc cacaccatcg caccattacc gaagcggtac atcaggaagg
    28621 cggcaaaata gccctgcaaa ttttgcatac cgggcgctac agctaccaac cgcatctagt
    28681 cgctccctcc gcattgcagg cccccatcaa ccgtttcgtt cctcacgaac tcacccatga
    28741 agagatcctg caactgatcg acgatttcgc ccactgcgcg caactggcac gggaggcagg
    28801 atacgacggt gtagaggtaa tggggtccga agggtatttg atcaacgaat ttctgacgct
    28861 gcgcaccaat cagcgtagtg accagtgggg cggcgattac cgcaaccgga tgcgatttgc
    28921 cgtagaagta gtgcgtgcgg tgcgcgaacg cgtcggcaac gacttcatta ttatctaccg
    28981 actgtcgatg ctcgacctgg tcgaagacgg cgggactttt gccgaaacgg tagagctggc
    29041 gcaggccatt gaagcggcgg gcgcgaccat tatcaacacc ggcattggct ggcatgaagc
    29101 acgtattccg accattgcca cgcccgtgcc gcgcggcgca tttacttggg tcacgcgcaa
    29161 actgaaaggc cacgtttcgc tgccgctggt gaccaccaac cggattaacg atccgcaggt
    29221 tgccgacgac attctctcgc gcggcgatgc cgatatggtg tcgatggcac gaccgtttct
    29281 tgctgatgcg gaactgctgt caaaagcgca atcggggcga gccgatgaga tcaacacctg
    29341 cattggctgc aatcaggcct gcctcgatca gatcttcgtt ggcaaagtca cctcgtgcct
    29401 ggtgaatcct cgcgcctgcc acgaaaccaa aatgccaatc cttcctgccg tgcagaaaaa
    29461 aaatctggcg gtggtcggtg cgggacctgc tgggctggcg tttgccatta acgcggcggc
    29521 gcgtgggcat caggtaacat tgtttgatgc ccacagcgag atcggcgggc agtttaatat
    29581 cgccaaacag atccccggca aagaggagtt ttacgaaacg ctgcgctatt accaccggat
    29641 gatcgaagtg acgggcgtga cgctgaaact caatcacacc gtgacggcgg atcagttaca
    29701 ggcgttcgat gaaacgatcc tcgccagtgg gatcgtgccg cgcgttccgc ctatcgacgg
    29761 gatcgatcat ccgaaggtat tgagttatct cgatgtactg cgcgacaaag cgccggtagg
    29821 caacaaggtt gccatcatcg gttgtggcgg gattggtttt gatacggcga tgtatttaag
    29881 tcagccgggc gaatccacca gccagaatat cgccgggttc tgtaatgagt gggggatcga
    29941 cagcagccta caacaggctg gtggcttaag cccgcaggga atgcagatcc cgcgtagccc
    30001 acggcaaatt gtgatgctcc agcgcaaagc cagcaaaccg ggacaggggt taggcaaaac
    30061 caccggctgg atccatcgca ctaccctgct ctcgcgcggt gtgaaaatga tcccaggcgt
    30121 cagttaccag aagatcgacg atgacgggct gcatgtggtg atcaacggcg aaacacaggt
    30181 attcgcggtg gacaatgtgg tgatctgcgc agggcaagag ccaaaccgcg cgctggcgca
    30241 accgctgatt gatagcggga aaactgtgca tttaattggc ggctgcgatg tggctatgga
    30301 gctggacgca cgacgggcaa ttgcccaggg aacacggctg gcgctggaga tttaaatcgt
    30361 tatttgaagc gccggatgca acgcatccgg cacgtcgcgt taatcaataa acaaggcggg
    30421 agaaatgccg aatcgcgttg ccaatttttt agcgtgttcc agcgttaatt tcctcttccc
    30481 gctcaaaacg cgtgacacca tagatttact gccaatttcc ggcagatcgg aaagggttaa
    30541 accatattga tccataaggg tacgaatcac ggctataccg ccaggcatgg cttgagccat
    30601 ggcattaaat tccgcaaatt cgggcgctga ttcttcccac gcggttattt tggcacacac
    30661 cagatccagc aaggggtttt caggatcgtt gagcagcaga tgatctacca gttccagcgc
    30721 ctgggtgtat tgttcctcgt tctgaatacc cgccagaaaa ggtgccaccg cagttagctt
    30781 ttctcctgct tgcaagatgt cagcaatcgc aatcattttt tccccttagt acgatgaaca
    30841 gcggtaaaga aatcgtattc tttatgcgtc ataacttcac gtatgtagca taaacgttga
    30901 acaacgctaa acaagagaat gtagaggatg gtgacaggga ttttttgttt tatggaagcg
    30961 cgattccaga actgggcaga cattgccgga tgcgacgctt gccgcgtctt atccggcctg
    31021 ggagtaacgt gctgaacgta ggccggataa ggcgttaacg ccgcatccgg caggtaactc
    31081 ccaatcttac cgacgacgcc ccagcttcac tgttttcagc accacaaatt tattattcgt
    31141 ggcgatggtg gtgcagttgc cgaaaatctt cttcagttta tggaagtaat ccaggtgacg
    31201 gttggcaacg atatacagtt cgccgttgat tttcaggcag cggcgggcgt ggtggaacat
    31261 ctcccaggcg acgttatcgg tcagcgcatg ttgctgatga aacggcgggt tgcagagcac
    31321 agcattaaag cggaaaggct ccactccgga gagcgcgttg ttgatcataa actcgcagcg
    31381 atccaacgcc tctggcatgt tggtttcaac attcattcgg ctggaagcga ccgccatcgg
    31441 cgattcatcg acaaacacca ctttcgcctg cgggttttta tcaagcagcg tcaggccaat
    31501 aacgccgtta ccacagccga gatcgacaat ctcaccttcg agattctctg gcagatgttg
    31561 cataaagaag cgtgcgccaa tatccagccc ggtgcgggag aagacattcg catggttgtg
    31621 gatagtccag tcagtacctt ccagcttcca gctaacggtc tgcggcgcat cggccagcgg
    31681 cggttcattg aaagtgcaat taatcaggcg cgcttttttc catgccagcg tagtggtggt
    31741 cgggccgagc actttctcga acagctccag cgtggaagtg tgaatgtcac gggctttggc
    31801 accggcaata atacgtgtat ccgacgtgac cactttgcgc agcgcacgca gttgctgttc
    31861 cagcaatgcc agtgttttcg gcactttgat cagcaccaca cccggctgtt gcgggtagtc
    31921 ggcggtgctg tcgagaaact tcacgctcga ctcatcaatc ccgttgaggc gtaaattctc
    31981 gcgcgtcgcc agttcactga tgtatgagtc gccaatgctg tacggcttat gttccgccag
    32041 cgcgcagctt aacgcaccaa aggcatcatt caggatcaac accgggccgc ggatttctgt
    32101 gtcgtccaac tgttgcagca aatattcatc cgccgcttcc cacgcctgta gcgggttaac
    32161 gtcatccgtc gccggaaaac gttgtagtgt cagtgaacgg aaaccgttgt ctaagtggct
    32221 catcggccct cctgaatgat aaaatttcga cgttgcccct gaaaagggtg cgtgagtata
    32281 ccctttttct ctttttcgtg ggcggttatg agcaatctta cttatcttca gggttatccc
    32341 gagcagctac tttcccaggt gcgaacgctg attaacgaac agcgtctggg cgatgtgctg
    32401 gcaaaacgct atccgggaac acacgactac gccaccgata aagccctctg gcaatatact
    32461 caggatctaa aaaatcagtt tctgcgcaat gccccgccga tcaataaagt gatgtatgac
    32521 aataagatcc atgtgctaaa aaacgcgctc gggttacata ccgctgtttc tcgtgtgcag
    32581 ggcggcaagc tgaaagcaaa agcagagatc cgcgtcgcta ctgtgtttcg caacgcgccg
    32641 gaaccgtttt tacgtatgat cgtggtgcac gaactggcgc acctgaagga gaaagagcat
    32701 aacaaagcgt tttaccagtt gtgctgccat atggagccgc agtaccacca gcttgagttc
    32761 gacacccggc tgtggctaac gcagttgtcg cttgggcagg acaaaatctg aaaatgatgt
    32821 caggcgttat cttttctttc tttagcgcct aaaatcgacc tccccccttt cgttaaggat
    32881 aacgaccatg cagccccgcc ttcttctccg tatttgtttt tctcgccgga ctcttaagat
    32941 tggctgtttg ttgttgctta tagcaggagc gaccattttt attgccgatc gcgttatggt
    33001 caacgccagc aagcagctga cctggagcga tgtcaacgca gttccggagc gtaacgtggg
    33061 cttattgctg ggggccaggc cgggtaatcg ctactttacc cgacgtattg ataccgcagc
    33121 agcgttgtac cacgccggaa aagtgaaatg gctactggta agtggcgata acggacgtaa
    33181 aaattacgat gaagcatcag gtatgcagaa ggctttgatc gctaaaggcg taccggcgaa
    33241 agtgatcttc tgcgactatg ccggattctc aacgctggat tcggtagtgc gtgccaaaaa
    33301 ggtttttggt gaaaaccata tcaccattat ctcgcaagaa tttcataacc aacgcgccat
    33361 ctggctggca aaacagtaca gcatcgacgc tatcggtttt aacgcgcctg acctcaatat
    33421 gaagcacggt ttttatactc aactgcggga gaaactcgct cgtgtcagcg ccgtgattga
    33481 cgccaaaatc ctccatcgcc agccgaaata tctcgggcca tcggtcatga ttgggccgtt
    33541 tagtgagcat ggctgtccgg caaaagaata atgcgtatct gcgcacgtcg aagatgaaaa
    33601 aggcgtgcta cattgacgac agaatccctt tatggagtat ccacgcgtta tgatacgttt
    33661 cgctgtgatt ggtacgaact ggatcactcg ccagttcgtc gaggccgccc atgagagcgg
    33721 taaatacaag ttaaccgccg tatattcccg cagccttgag caggctcagc acttcgccaa
    33781 tgatttttct gtcgagcatc tgtttacctc gctggaagcg atggcggaaa gcgatgccat
    33841 tgacgcggtg tatattgcca gcccgaatgc cctgcacttt tcccagacac aacttttcct
    33901 tagccataaa attcatgtga tttgcgagaa accactggcg tcgaatctgg cggaagtgga
    33961 tgccgccatt gcctgtgcgc gggaaaatca ggtggtgctg tttgaggcat ttaaaaccgc
    34021 ctgcctgccg aactttcatt tgctgcgcca ggcgctgccg aaagtcggca aattgcgtaa
    34081 agtctttttc aactattgcc agtattcctc gcgctatcaa cgttacctgg acggtgaaaa
    34141 tcccaacacc tttaatccgt cattctctaa cggttcaatt atggatatcg gcttttactg
    34201 cctggcgtcg gcggtggcgt tatttggtga gccgaaaagc gtgcaggcaa ccgccagttt
    34261 gctggcaagc ggtgtagatg cccacggcgt ggtggtgatg gattacggtg atttcagcgt
    34321 caccttgcag cactcaaaag tcagtgattc tgtcctggcg agcgagattc agggcgaagc
    34381 aggatcgctg gtgattgaaa aactgtctga atgccagaaa gtgtgcttcg tgccgcgtgg
    34441 cagccaaatg caggatctca cccagccgca gcatattaat accatgctct acgaagcaga
    34501 gctgttcgct accctggtgg atgagcatct ggtggatcat ccggggctgg cggtcagtcg
    34561 catcaccgcc aaactgctga ccgagatccg ccgccagact ggggtgattt ttccggcaga
    34621 tagcgtaaaa ctataattgc caaagtaaaa cagtgtaaaa ggtatgtaac agaccattga
    34681 ctggctgaat ggtctgtcat actttgttac ctgcaaaggg gagtaacttc attgccggtc
    34741 gatcgtcatt acgatgtgtg aaaaaccaca tccggtcacc gggcaacccg aaaggaatac
    34801 gcagacgtat tccttttttg ttgtaagtga gaccttgccg gaaggcgagg tctatgcata
    34861 aaaagcagcg gctgacgtct tccgacgttg gccgtttttt tatgtgtaag gaacttctat
    34921 gaatactgtc ggcacgccgt tgctatgggg cggattcgct gttgttgtcg ccattatgct
    34981 ggctatcgac ctgttgttgc aggggcgtcg tggggcacat gccatgacca tgaaacaggc
    35041 tgcggcctgg tcgctggtct gggtgacgct gtcgttactg tttaacgccg ctttctggtg
    35101 gtatctggtg caaaccgaag gtcgcgccgt tgccgatcca caggcactgg cctttctcac
    35161 aggttatctg attgagaaat cgctggcggt cgataacgtc tttgtctggc tgatgttgtt
    35221 cagctatttc tctgttccgg cggcattaca acgccgcgtg ctggtgtatg gcgtgctcgg
    35281 ggcgattgtt ctgcgtacca tcatgatctt cactggcagc tggctaattt cgcagttcga
    35341 ctggatcctg tatatcttcg gtgccttcct gctgtttacc ggcgtgaaga tggcgctcgc
    35401 ccatgaagat gaatcaggca ttggcgacaa gccgctggtg cgctggctac gcggtcattt
    35461 gcgcatgacc gacaccatcg acaacgagca tttctttgtg cgtaagaatg gattgctgta
    35521 cgccacaccg ctgatgctgg tactgattct ggtggaattg agcgacgtga ttttcgccgt
    35581 ggatagcatt ccggctatct tcgccgtgac cactgacccg ttcattgtgc tgacctcaaa
    35641 cctgtttgcg atcctcggcc tgcgtgcgat gtatttcctg ctggcgggcg tagcagagcg
    35701 tttctcgatg ctcaaatatg gcctggcggt gattctggtg tttatcggta tcaagatgct
    35761 gattgtcgac ttctaccata ttccaatcgc cgtctcgctg ggcgtggtgt ttggcattct
    35821 ggtgatgacg tttattatca acgcctgggt gaattatcgg catgataagc agcggggaga
    35881 atgatccgct gtatattaag ccattactag agataactca ttccagacaa aggtgagcta
    35941 ttaattatgt aatcggctta tagctcttta caaaaagacc gcacgggtgt tggtggcgtc
    36001 aaaatcgccg aagcgttccc tggaggccgg ggcgacgcgc atggatgcgc ggcgagggcg
    36061 catcttacag ggacgttacc ttgcgcccga cccggtagcc gcaagggata agctgaggga
    36121 accgcgcagc ggcgattttg ttcgccag
//
LOCUS       DAZGAN020000041        35572 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGAN020000041 DAZGAN020000000
VERSION     DAZGAN020000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.041"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(62..1306)
                     /gene="mtr"
                     /locus_tag="ACHKYO_RS11175"
                     /old_locus_tag="pgaptmp_002235"
     CDS             complement(62..1306)
                     /gene="mtr"
                     /locus_tag="ACHKYO_RS11175"
                     /old_locus_tag="pgaptmp_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HGU4429081.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPI
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1460..3349)
                     /gene="deaD"
                     /locus_tag="ACHKYO_RS11180"
                     /old_locus_tag="pgaptmp_002236"
     CDS             complement(1460..3349)
                     /gene="deaD"
                     /locus_tag="ACHKYO_RS11180"
                     /old_locus_tag="pgaptmp_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HGU4429082.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3342..3422)
                     /gene="yrbN"
                     /locus_tag="ACHKYO_RS11185"
                     /old_locus_tag="pgaptmp_002237"
     CDS             complement(3342..3422)
                     /gene="yrbN"
                     /locus_tag="ACHKYO_RS11185"
                     /old_locus_tag="pgaptmp_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HGU4429083.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3529..4413)
                     /gene="nlpI"
                     /locus_tag="ACHKYO_RS11190"
                     /old_locus_tag="pgaptmp_002238"
     CDS             complement(3529..4413)
                     /gene="nlpI"
                     /locus_tag="ACHKYO_RS11190"
                     /old_locus_tag="pgaptmp_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HGU4429084.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDCLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYHDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4522..6657)
                     /gene="pnp"
                     /locus_tag="ACHKYO_RS11195"
                     /old_locus_tag="pgaptmp_002239"
     CDS             complement(4522..6657)
                     /gene="pnp"
                     /locus_tag="ACHKYO_RS11195"
                     /old_locus_tag="pgaptmp_002239"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGU4429085.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6904..7173)
                     /gene="rpsO"
                     /locus_tag="ACHKYO_RS11200"
                     /old_locus_tag="pgaptmp_002240"
     CDS             complement(6904..7173)
                     /gene="rpsO"
                     /locus_tag="ACHKYO_RS11200"
                     /old_locus_tag="pgaptmp_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGU4429086.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7322..8266)
                     /gene="truB"
                     /locus_tag="ACHKYO_RS11205"
                     /old_locus_tag="pgaptmp_002241"
     CDS             complement(7322..8266)
                     /gene="truB"
                     /locus_tag="ACHKYO_RS11205"
                     /old_locus_tag="pgaptmp_002241"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGU4429087.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8266..8667)
                     /gene="rbfA"
                     /locus_tag="ACHKYO_RS11210"
                     /old_locus_tag="pgaptmp_002242"
     CDS             complement(8266..8667)
                     /gene="rbfA"
                     /locus_tag="ACHKYO_RS11210"
                     /old_locus_tag="pgaptmp_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGU4429088.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8831..11503)
                     /gene="infB"
                     /locus_tag="ACHKYO_RS11215"
                     /old_locus_tag="pgaptmp_002243"
     CDS             complement(8831..11503)
                     /gene="infB"
                     /locus_tag="ACHKYO_RS11215"
                     /old_locus_tag="pgaptmp_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGU4429089.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11528..13015)
                     /gene="nusA"
                     /locus_tag="ACHKYO_RS11220"
                     /old_locus_tag="pgaptmp_002244"
     CDS             complement(11528..13015)
                     /gene="nusA"
                     /locus_tag="ACHKYO_RS11220"
                     /old_locus_tag="pgaptmp_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGU4429090.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13043..13495)
                     /gene="rimP"
                     /locus_tag="ACHKYO_RS11225"
                     /old_locus_tag="pgaptmp_002245"
     CDS             complement(13043..13495)
                     /gene="rimP"
                     /locus_tag="ACHKYO_RS11225"
                     /old_locus_tag="pgaptmp_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGU4429091.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13702..13778)
                     /locus_tag="ACHKYO_RS11230"
                     /old_locus_tag="pgaptmp_002246"
     tRNA            complement(13702..13778)
                     /locus_tag="ACHKYO_RS11230"
                     /old_locus_tag="pgaptmp_002246"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13742..13744),aa:Met,seq:cat)
     gene            14126..15469
                     /gene="argG"
                     /locus_tag="ACHKYO_RS11235"
                     /old_locus_tag="pgaptmp_002247"
     CDS             14126..15469
                     /gene="argG"
                     /locus_tag="ACHKYO_RS11235"
                     /old_locus_tag="pgaptmp_002247"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HGU4429092.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15477..17101)
                     /gene="yhbX"
                     /locus_tag="ACHKYO_RS11240"
                     /old_locus_tag="pgaptmp_002248"
                     /pseudo
     CDS             complement(15477..17101)
                     /gene="yhbX"
                     /locus_tag="ACHKYO_RS11240"
                     /old_locus_tag="pgaptmp_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(17560..17646)
                     /locus_tag="ACHKYO_RS11245"
                     /old_locus_tag="pgaptmp_002249"
     tRNA            complement(17560..17646)
                     /locus_tag="ACHKYO_RS11245"
                     /old_locus_tag="pgaptmp_002249"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17610..17612),aa:Leu,seq:gag)
     gene            complement(17661..17993)
                     /gene="secG"
                     /locus_tag="ACHKYO_RS11250"
                     /old_locus_tag="pgaptmp_002250"
     CDS             complement(17661..17993)
                     /gene="secG"
                     /locus_tag="ACHKYO_RS11250"
                     /old_locus_tag="pgaptmp_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGU4429093.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(18221..19558)
                     /gene="glmM"
                     /locus_tag="ACHKYO_RS11255"
                     /old_locus_tag="pgaptmp_002251"
     CDS             complement(18221..19558)
                     /gene="glmM"
                     /locus_tag="ACHKYO_RS11255"
                     /old_locus_tag="pgaptmp_002251"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGU4429094.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(19551..20399)
                     /gene="folP"
                     /locus_tag="ACHKYO_RS11260"
                     /old_locus_tag="pgaptmp_002252"
     CDS             complement(19551..20399)
                     /gene="folP"
                     /locus_tag="ACHKYO_RS11260"
                     /old_locus_tag="pgaptmp_002252"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGU4429095.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(20489..22423)
                     /gene="ftsH"
                     /locus_tag="ACHKYO_RS11265"
                     /old_locus_tag="pgaptmp_002253"
     CDS             complement(20489..22423)
                     /gene="ftsH"
                     /locus_tag="ACHKYO_RS11265"
                     /old_locus_tag="pgaptmp_002253"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGU4429096.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(22523..23152)
                     /gene="rlmE"
                     /locus_tag="ACHKYO_RS11270"
                     /old_locus_tag="pgaptmp_002254"
     CDS             complement(22523..23152)
                     /gene="rlmE"
                     /locus_tag="ACHKYO_RS11270"
                     /old_locus_tag="pgaptmp_002254"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HGU4429097.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            23278..23571
                     /gene="yhbY"
                     /locus_tag="ACHKYO_RS11275"
                     /old_locus_tag="pgaptmp_002255"
     CDS             23278..23571
                     /gene="yhbY"
                     /locus_tag="ACHKYO_RS11275"
                     /old_locus_tag="pgaptmp_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGU4429098.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(23727..24203)
                     /gene="greA"
                     /locus_tag="ACHKYO_RS11280"
                     /old_locus_tag="pgaptmp_002256"
     CDS             complement(23727..24203)
                     /gene="greA"
                     /locus_tag="ACHKYO_RS11280"
                     /old_locus_tag="pgaptmp_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGU4429099.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            24451..25884
                     /gene="dacB"
                     /locus_tag="ACHKYO_RS11285"
                     /old_locus_tag="pgaptmp_002257"
     CDS             24451..25884
                     /gene="dacB"
                     /locus_tag="ACHKYO_RS11285"
                     /old_locus_tag="pgaptmp_002257"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU4429100.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(25924..27096)
                     /gene="cgtA"
                     /locus_tag="ACHKYO_RS11290"
                     /old_locus_tag="pgaptmp_002258"
     CDS             complement(25924..27096)
                     /gene="cgtA"
                     /locus_tag="ACHKYO_RS11290"
                     /old_locus_tag="pgaptmp_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HGU4429101.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(27112..28077)
                     /gene="yhbE"
                     /locus_tag="ACHKYO_RS11295"
                     /old_locus_tag="pgaptmp_002259"
     CDS             complement(27112..28077)
                     /gene="yhbE"
                     /locus_tag="ACHKYO_RS11295"
                     /old_locus_tag="pgaptmp_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4429102.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(28204..28461)
                     /gene="rpmA"
                     /locus_tag="ACHKYO_RS11300"
                     /old_locus_tag="pgaptmp_002260"
     CDS             complement(28204..28461)
                     /gene="rpmA"
                     /locus_tag="ACHKYO_RS11300"
                     /old_locus_tag="pgaptmp_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGU4429103.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(28482..28793)
                     /gene="rplU"
                     /locus_tag="ACHKYO_RS11305"
                     /old_locus_tag="pgaptmp_002261"
     CDS             complement(28482..28793)
                     /gene="rplU"
                     /locus_tag="ACHKYO_RS11305"
                     /old_locus_tag="pgaptmp_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGU4429104.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            29052..30023
                     /gene="ispB"
                     /locus_tag="ACHKYO_RS11310"
                     /old_locus_tag="pgaptmp_002262"
     CDS             29052..30023
                     /gene="ispB"
                     /locus_tag="ACHKYO_RS11310"
                     /old_locus_tag="pgaptmp_002262"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HGU4429105.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            30250..30528
                     /gene="sfsB"
                     /locus_tag="ACHKYO_RS11315"
                     /old_locus_tag="pgaptmp_002263"
     CDS             30250..30528
                     /gene="sfsB"
                     /locus_tag="ACHKYO_RS11315"
                     /old_locus_tag="pgaptmp_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HGU4429106.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(30576..31835)
                     /gene="murA"
                     /locus_tag="ACHKYO_RS11320"
                     /old_locus_tag="pgaptmp_002264"
     CDS             complement(30576..31835)
                     /gene="murA"
                     /locus_tag="ACHKYO_RS11320"
                     /old_locus_tag="pgaptmp_002264"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGU4429107.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(31890..32144)
                     /gene="ibaG"
                     /locus_tag="ACHKYO_RS11325"
                     /old_locus_tag="pgaptmp_002265"
     CDS             complement(31890..32144)
                     /gene="ibaG"
                     /locus_tag="ACHKYO_RS11325"
                     /old_locus_tag="pgaptmp_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HGU4429108.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(32304..32597)
                     /gene="mlaB"
                     /locus_tag="ACHKYO_RS11330"
                     /old_locus_tag="pgaptmp_002266"
     CDS             complement(32304..32597)
                     /gene="mlaB"
                     /locus_tag="ACHKYO_RS11330"
                     /old_locus_tag="pgaptmp_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HGU4429109.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(32597..33232)
                     /gene="mlaC"
                     /locus_tag="ACHKYO_RS11335"
                     /old_locus_tag="pgaptmp_002267"
     CDS             complement(32597..33232)
                     /gene="mlaC"
                     /locus_tag="ACHKYO_RS11335"
                     /old_locus_tag="pgaptmp_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HGU4429110.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(33251..33802)
                     /gene="mlaD"
                     /locus_tag="ACHKYO_RS11340"
                     /old_locus_tag="pgaptmp_002268"
     CDS             complement(33251..33802)
                     /gene="mlaD"
                     /locus_tag="ACHKYO_RS11340"
                     /old_locus_tag="pgaptmp_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HGU4429111.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(33807..34589)
                     /gene="mlaE"
                     /locus_tag="ACHKYO_RS11345"
                     /old_locus_tag="pgaptmp_002269"
     CDS             complement(33807..34589)
                     /gene="mlaE"
                     /locus_tag="ACHKYO_RS11345"
                     /old_locus_tag="pgaptmp_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HGU4429112.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(34597..35406)
                     /gene="mlaF"
                     /locus_tag="ACHKYO_RS11350"
                     /old_locus_tag="pgaptmp_002270"
     CDS             complement(34597..35406)
                     /gene="mlaF"
                     /locus_tag="ACHKYO_RS11350"
                     /old_locus_tag="pgaptmp_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HGU4429113.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
BASE COUNT         8724 a         9787 c         8478 g         8583 t
ORIGIN      
        1 gataagcgaa gcgcatcagg cattgtgtag gcagcagaaa tgtcggataa ggcaccgctg
       61 attactgata caccggcagt aaattaaagc tcgataaaat atgcaccagt gcgttgccga
      121 cgccaaacac cagaatcagc gcaatcatcg gcttgccacc ccagacgcgg aatttcgggc
      181 tgccaaagcg tttacgcgat gcgcgggcta acagcgccgg aacaattgcc gcccagatgg
      241 tagctgctaa accagcataa ccaatggcgt acaggaatcc gttcgggaac agtagaccac
      301 ccacaatagg cggggcaaag gtcagcaatg ccgttttcaa gcgccccagg gctgagtcat
      361 cgaaaccaaa cagatctgcc agatagtcaa acaaacccag cgttacgcca aggaacgaac
      421 tcgctaccgc aaagtttgag aacacgacca gcagaaggtc caggctacgg ctgttcagca
      481 cgccgcttaa tgcctgtacc agcacatcaa tattaccgcc cttctctgca ataccgataa
      541 actccggacg cgggatgtta cccatcgtcg ccagcaacca gatggtatac agcgccagtg
      601 ccatcagcgt accgtacacc aggcatttca cgatggtttt cggatctttg ccgtaatact
      661 tcatcaggct tggcacgtta ccgtgataac caaacgacgc caggcagaac ggcaatgtca
      721 tcagcagata cggcgcataa gatgcattgc tttctgcaac gttgaacaat gtcgcaggct
      781 gcacatgccc caacaggctg ccaaaggtga ggaagaaggt aatgactttc gcccccagca
      841 caatcgccgt catgcggctg acggccttag tactcaacca caccacaaac gctaccagca
      901 gtgcaaaacc aaaacccgcc gcccgtgccg ggacgtttag cgacatctct gcgaaggtgt
      961 gatgcagaat tgaaccactg gcagaaatat aggcataggt caggatatag agcacaaagg
     1021 caatggaaat accgttgacc acgttccagc ctttgcccag caaatctttg gtgatggtgt
     1081 caaaactcga accgattcgg taattcaggt tagcttccag aatcatcaag ccggaatgca
     1141 gcatacagaa ccaggtaaag atcagcgccg ccattgacca gaaaaaccac gccccggaca
     1201 tgaccactgg cagagaaaac atccctgcgc caataatggt gccgccgata atcaccacgc
     1261 cgccaagcag cgacggtgac gtttgggtgg tggttagtgt tgccatgagg gcttctctcc
     1321 agtgaaaaat agtgcgactg cgttgttatg cattgcactg taccagtaca cgagtacaaa
     1381 agacagaaaa aaagccccga tggtaaaaat cggggctgta tatattattt tacagattgt
     1441 gttcgctgtt cagcgatgat tacgcatcac caccgaaacg acgacgaccg gtagagtcat
     1501 cacgacgcgg agcgcggcct tcacgacgtt cgccgctaaa acgacgacca tcaccacggc
     1561 caccttcacg gcgttcaccg ctgaagttac gaccgccttc acgacgttcg ccaccgaaac
     1621 cacgaccacc gccacgacgc tcaccgccag tatgcggctg tgcatcgccc agtaactgca
     1681 tgttcatcgg cttgttgaga atgcgagtgc gcgtaaagtg ttgcagcact tcacccggca
     1741 tacctttcgg cagttcgatg gtggagtgag aagcaaacag cttgatgtta ccaatgtaac
     1801 ggctgctgat gtcgccttcg ttagcaatcg caccaacgat atgacgaact tcaacaccat
     1861 catcgcggcc cacttcaatg cggtacagct gcatatcgcc aacatcacga cgttcacgac
     1921 gcggacgatc ttcacggtca ccacgcgggc cacggtcgtt acgatcgcgc ggaccacggt
     1981 catcacggtc acggaattca cgtttcggac gcatcggcgc atctggcggt acgatcagag
     2041 tacgttcacc ctgtgccatt ttcagcagtg ccgcagccag agtttcgaga tccagctctt
     2101 caccttctgc agtcggctga attttgctca gcagtgcgcg gtattgatcc agatcgctgc
     2161 tttccagctg ctgctgtact ttagcggcga atttttccag acggcgtttg cccagcagtt
     2221 ctgcgttcgg cagttctact tccggaatag tcagcttcat agtacgttca atgttgcgca
     2281 gcagacgacg ctcgcggttc tcaacgaaca gcagcgcgcg gccagcacga cccgcacgac
     2341 cggtacgacc gatacggtga acgtaagact cagaatccat cgggatatcg tagttaacta
     2401 ccaggctgat acgctcaacg tccaggccac gggctgcaac gtcggtcgca atcaggatgt
     2461 ccagacgacc atctttcagg cgttccagtg tctgctcacg cagcgcctgg ttcatgtcac
     2521 cgttcagcgc ggcactgttg tagccgttac gctcaagagc ttcagccact tccagagtcg
     2581 catttttggt acgaacgaag ataatcgccg catcaaaatc ttccgcttcc aggaaacgta
     2641 ccagtgcttc gtttttacgc ataccccaga cagtccagta gctctggctg atgtcaggac
     2701 gggtagtcac gctggactga atgcgcactt cctgcggctc tttcataaag cggcgggtaa
     2761 tgcgacgaat cgcttccggc atggttgcag agaacagagc ggtctgatga ccttccggga
     2821 tctgcgccat aatggtttca acgtcttcga tgaagcccat gcgcagcatt tcgtcagctt
     2881 catccagaac cagaccgctc agtttagaga ggtccagagt gccacgtttc aggtggtcca
     2941 gcagacggcc cggagtaccg acaacgatct gcggcccctg acgcagggcg cgtaattgca
     3001 cgtcataacg ctggccgccg tatagagcaa ccacatttac gccgcgcatg tgtttagaga
     3061 aatccgtcat tgcttcagca acctgtaccg ccagttcgcg ggtcggtgcc agcaccagaa
     3121 tctgtggtgc tttcagctca ggatcaagat tctgcaacag aggtaaagag aatgctgcag
     3181 tttttccgct ccccgtctgg gccataccca gaacgtcgcg gccattcagc agatgtggaa
     3241 tacactctgc ctgaattgga gatggttttt cgtaacccag atcgttaagg gcttcaagga
     3301 taggagcctt caggcccaga tctgcaaaag tggtttcgaa ttcagccatg tagtacgtgt
     3361 gcctcaaaat taatggcggc cagtctacat aactcatcat gaaattgatc agcaattttc
     3421 attgaaaagt gtgaaccggc tcaaagtagg tgtattaacg aacaacaacg ccctcacccg
     3481 ttaaggtgat ggcaatcaaa aaagattacg ggctgatgtg tacgtcagct attgctggtc
     3541 cgattctgcc aggtcatctt ggtcctggcc caggagcgat aattccaaca atgcgtatcg
     3601 gtgctcaaca aagttatgaa cgttgttggc aaccgccagt ttgaacagtg ccgtggcgct
     3661 gtccaaatcc cccagactta ggtagtactt acctaaatag aagttggttt cactgagatg
     3721 ctcagcgagc gaggtgttat ccgttgcgtc cgccttgagc ctttccatta acgtttgttc
     3781 gctaatgttg cccaggtaga actcgacaat gttccatccc cactgttcct tatccgattt
     3841 ttcgaagtgc tgtttcaaca cttctttagc ctgcttctca tcgagcttct gctcggcgag
     3901 ataaagccac agactacgga aaggatcatt gggatcgtcg tgataaaacg ccagcagatc
     3961 atcttgcgct aacttgtcac gaccgccgta atataatgcg atcccgcgat tcaagtgcgc
     4021 gtagttgtaa gttggatcaa gctcaagtac agaatcaaac gcttcatagg cagcatcaaa
     4081 attgcctgcc tgcgttaaat atatgcctaa gtaattgaat acttcaggca tatccggtcg
     4141 gattgccagc gcttgcgaaa aatcgttacg cgctaatgcc ctcagaccga gacaatcata
     4201 caacactccg cgctcatata aaagctgtgc gcgttcgtca tcggttaaag cccgactggc
     4261 aaggatttgt tccatacgtg ccagaatcac ttcctgctgt aaagtcggtt gcaatggtac
     4321 cgcgaggact tcacttttac gccaggaagt attactgcat cctgcaagcg taagtgctgt
     4381 cgcaacgaaa caccagcgca aaaaaggctt catttcccac tcccgaagac cacggttgaa
     4441 tgaacgtcct gttcccggtt gctaacaagg cgtcctgccc ggttaaaagc cccccgccgc
     4501 agcggagggc aaatggcaac cttactcgcc ctgttcagca gccggagctt ccggtgctgc
     4561 agcaggttga gactgctcag tcgcttcttt aatgctcaga cggatacggc cctggcgatc
     4621 aacttccaga actttcaccg gtacttcctg acccatctgc aggtaatcgg tcactttctc
     4681 aacgcgtttg tcagcgattt gagagatgtg gaccagacct tctttaccgc cgccgatggc
     4741 aacaaatgcg ccaaagtcaa cgatacgggt cactttacca gtgtagacgc ggcccacttc
     4801 gatttctgca gtgatctctt cgatacgacg aatagcatgt ttcgctttct cgccgtcggt
     4861 cgctgcgatc ttcacagtac cgtcatcttc gatttcgatg gtagtgccag tttcttcggt
     4921 cagggcacgg ataacagagc cgcctttacc gataacgtct ttgatcttat ccgggttgat
     4981 cttgatggta tggatacgcg gtgcgaactc agagatatcg ccacgcggcg cgttgatcgc
     5041 ctgttccatt acgcccagga tatgcagacg cgcaccttta gcctggttca gcgcaacctg
     5101 catgatctct ttggtgatac cttcaatttt gatatccatc tgcagtgcag agataccgtc
     5161 gcgggaacct gcaactttga agtccatatc gcccaggtga tcttcgtcgc ccaaaatgtc
     5221 agacagtaca acgtagttgt cgccttcttt caccagaccc attgcgatac ccgcaacggc
     5281 agctttgatt ggcacacctg cgtccatcag cgccagagac gcgccgcaca cggaagccat
     5341 agaagaagaa ccgttggatt cggtgatttc agacactaca cgtacggtgt acgggaattt
     5401 gtccatatcc ggcatgactg ccagcacgcc gcgcttcgcc agacgaccgt gaccaatttc
     5461 acgacgcttc ggagaaccga ccatgccggt ttcgcctacg gagtacggag ggaagttgta
     5521 gtggaacagg aaggtatcgg tacgttcgcc catcagttca tcaagaacct gcgcgtcacg
     5581 agcggtaccc agcgttgcag tgaccagtgc ctgcgtttca ccgcgggtga acagcgcaga
     5641 accgtgagta cgcggcagca cgccagtacg cacatccaga ccacggatca tatctttttc
     5701 acgaccgtcg atacgcggtt cgcctgccag tacgcggcta cgaacaacgt ttttctcgat
     5761 agcgtgcaga atttcaccca gttcgttttc gtccagggtt tcgtcttcag caagcagcgt
     5821 cgcgatggtt tcagatttga tgacatcaac ctgcgcataa cgctcttgtt tgtcggtgat
     5881 gcggtaagca tcgctcaggc gagcttcagc cagtgcagca acgcgcgcgt tcagcgcttc
     5941 gtttaccggc tccggctgcc agtcccaacg tggtttaccg gcttctttca ccagttcatt
     6001 gatgttctga ataacaacct gctgttgttc atgaccgaac actactgcgc ccagcatctg
     6061 gtcttcgctc agcagttcag cttcagattc aaccatcaga acagcggctt cagtaccggc
     6121 aacaaccaga tccagtttgc tctctttcag ctcgtcctga gtcgggttca gtacgtactg
     6181 gtcattgatg taacctacgc gagcagcacc aatcggacca ttgaacggaa taccagacag
     6241 agacagtgct gcggaagcac caatcatcgc gacgatatcc gggttaactt gcgggttaac
     6301 agaaaccacg gtggcgataa cctgaacttc gttgacgaag ccttccggga acagcgggcg
     6361 aatcgggcgg tcaatcagac gcgcgatcag ggtttcgcct tcgcttgggc ggccttcacg
     6421 acggaagaag ctacccggga tacgaccagc agcgtaggta cgctcctgat agttaacggt
     6481 cagtgggaag aagtcctgac ctggtttggc ttttttctgg ccaacaacgg taacaaatac
     6541 cgcggtgtca tccatgctaa ccataacagc ggcagtagcc tgacgagcca tcatgccggt
     6601 ttccagagtc acggtgtgtt ggccgtactg gaatttacga acgatcggat taagcaatgt
     6661 aatatccttt ctctttctta gacagtacct tacggcactg gtgttaatac ccgatcttct
     6721 gcgcatcctc gcgactaatg acaaccctaa cccagctcta tgtgggtaaa gcctctcatt
     6781 agccgcgcga acctctgcaa cggaagatca ttcatagcaa caatacatta gtttccagtg
     6841 aattgctgcc gtcagcttga aaaaaggggc cactcaggcc cccttttctg aaactcgcaa
     6901 gaattagcga cgcagaccca ggcgctcgat gagctgggtg taacgtgcta cgtctttacg
     6961 tttcaggtag tcgagcagtt tacgacgctg agaaaccatg cgcagcagac cacgacggct
     7021 gtggtgatct tttttgtgct ctgcaaagtg gccctgcagg tggttgatct gtgcagtcag
     7081 cagtgctacc tgaacttcgg tagaaccggt gtcgtttgcg tcacgaccaa actcagaaac
     7141 gattttagct gttgcttcag tacttagaga cattttaaaa ctccaaagta tatagaatga
     7201 aaggacgccg atctctaatt cagcgatccc agtgtacgtt acgcaaagtt ttaaacaatt
     7261 tacgcgacgt taagcggcag tattctactc gtagcgacct gttatcgcaa gacggttaac
     7321 attacgccgg gtattcaacc accaggcgac gaggcgcaac gcggccttca tcgtcaattt
     7381 cgcccatacc gataaatttg ccgttctcac cttctgtaac gcgaaccagt ccttccagcg
     7441 gcgcaccgga tgtacgaact gggttaccat ttttgaagta aacagaagac gttaacggaa
     7501 gattcaccac cgggtagtcc gaagctggac tgtccattgg catcagtaat ggatcaagta
     7561 actccgcagc cggaatatcc tgctgttcgg cttgttcgac cagttcacgc agatgttcca
     7621 gggtcaccat ccgttcaacc ggatatttac ttaccgccag acgacgcagg taaataacat
     7681 gcgcgccaca accgagtttt tcacccaggt catcaatgat ggtgcggata taagtgcctt
     7741 ttgagcagtg aatttccagc tccagctcat tgccttcgtg gcgaataaac agcaattcat
     7801 aaacggtaat cgggcgcgct tcacgcggta cttcaatgcc ctgacgcgca tattcgtaca
     7861 gttttttacc ctgatatttc agtgctgaat acatcgaagg gatctgttcg atatcgccac
     7921 ggaaagtatc cagcgccgcc gccagttgct ctgcgctaaa ggttaccgga cgttcttcaa
     7981 cgatttgtcc gtcggcatca gaagtatcgg tacgctgtcc aagacgcgca atgacccgat
     8041 agcgtttgtc ggagtccagc agatactggg aaaacttcgt cgcttccccg aggcaaattg
     8101 gcaacatgcc agttgccagc gggtccagcg caccggtatg cccggcacgg ttggcgttat
     8161 aaatccgttt cactttttgc agcgcatcat tgctggacat accctgcggt ttatccagca
     8221 acaaaacgcc gttaatgtcg cgaccgcgac gacgaggacg actcattagt cctccttgct
     8281 gtcgtccggg ttaacacgac gttcttcgtc atgtttgacc acgctggtca ccaggtttga
     8341 catgcgcatc ccttcaacca gagagttgtc gtagaagaag gtcagttccg gcacgatacg
     8401 caggcgcatc gctttcccca gcaggctgcg gatgaaacca gaagcttctt gcaacgcttt
     8461 gatgcccgct ttaaccgcgt cttcatcttt gtcgttgagg aacgtcacat atactttggc
     8521 atacgccagg tcgcgagaca tttcgacacc ggaaacggtg gtcatcatgc ccaggcgagg
     8581 atctttaatt tcacgctgca ggatgagagc gatctctttt tgcatttcct gcgctacgcg
     8641 ctgcgggcga ccaaattctt tcgccataat aaattctcct gacaaaaaag gggctgttag
     8701 ccccttttta aaattaattt caggtggaag ggctgttcac gttggcctga taagacgcgc
     8761 cagcgtcaca tcaggcaacg gctgccggat gcggcgtaaa agccttatcc ggcctacaac
     8821 ccagacaacc ttaagcgatg gtacgttgga tttcgatgat ctcgaatact tcgatcacat
     8881 cgccagtgcg gacatcgttg tagttcttaa cgccgatacc acattccata ccgttacgga
     8941 cttcgttaac gtcatctttg aagcggcgca gggactccag ctcgccttcg tagataacca
     9001 cgttgtcacg cagaacgcgg atcgggttgt gacgtttaac cacaccttcg gtaaccatac
     9061 agcctgcgat ggcaccaaat ttcggcgatt tgaacacgtc acgaacttcc gccagaccga
     9121 taatctgctg tttcagttcc ggagacagca taccgctcat cgccgctttc acttcgtcaa
     9181 tcaggttata gatgacggag tagtaacgca gatccaggct ttccgcttca atcactttac
     9241 gtgcagaggc atcagcacgt acgttgaagc caaccaggat ggcgttggac gccgcagcca
     9301 gggtggcgtc ggtttcggtg ataccaccta cgccagaacc gatgatcttc actttaactt
     9361 cgtcagtaga cagtttcagc aaggagtcgg agatcgcttc cacagaaccc tgtacgtctg
     9421 ccttcaggac gatgttcact tcgtgaactt cgccttcggt catgttggcg aacatgttct
     9481 cgagtttaga tttctgctga cgcgccagtt taacttcgcg gaatttaccc tgacgataca
     9541 gtgcaacttc acgcgctttc ttctcgtcac gtacaacggt aacttcatca cccgcagccg
     9601 gtacgccgga caggccgagg atttccaccg gaatggacgg acccgcttcc agcacttcct
     9661 gacccagttc gttacgcatc gcacgaacac gaccgtattc gaagccacac agaacgatat
     9721 cgcccttgtg cagagtacct tcacgtacca gaacggtagc aaccggacca cgacctttat
     9781 cgaggaagga ttcgataacc gcaccgctcg ccataccttt acgtaccgct ttcagctcca
     9841 gaacttccgc ctgcagcagg atagcgtcca gcagttcatc gatgccggta cccgctttcg
     9901 cagatacgtg tacgaactgg ctttcaccgc cccactcttc cggcaggatg ccatactggg
     9961 agagttcgtt cttaacacga tccggatcgg cttctggttt atcaatcttg ttcactgcaa
    10021 ccaccaccgg tacctgcgcc gctttcgcgt gctggattgc ttcgatggtc tgcggcatca
    10081 cgccgtcgtc ggcagcaaca accaggacta cgatgtccgt tgcctgcgca ccacgtgcac
    10141 gcattgaagt aaacgcggcg tgccccgggg tgtccaggaa ggtgatcatg ccgttttcag
    10201 tttcaacgtg gtatgcacca atgtgctggg taatgccgcc cgcttcgcca gaggcaactt
    10261 tcgttgaacg aatgtagtcc agcagagagg ttttaccgtg gtcaacgtga cccatgatgg
    10321 tcacaaccgg cgcgcgcggt tcagccgcag cacccgtgtc acggtcgctc attaccgcct
    10381 cttccagctc gttttcacga cgcaggataa ctttgtggcc catctcttca gcaaccagct
    10441 gtgcggtttc ctgatcgata acctggttga tggttgccat tgcgcccagt ttcatcatcg
    10501 ctttgatgac ctgagagcct ttaaccgcca tcttgttcgc cagttcgcca acggtgatgg
    10561 tttcgccgat cacaacgtca cggttaacgg cctgagctgg cttctggaag ccttgctgca
    10621 gggaagaacc tttacgtttt ccgcctttac cgccacgtac tgctgcgcgt gcttcttcac
    10681 gatcagcttt tgattcagcg tgtttgttgc ctttcttcgg acgcgctgct ttcgcgttac
    10741 gaccacggcc acgaccgcct tcgacttcac gatcgctttc gtcttctgcc tggcgagcat
    10801 gttgagaagt agtgacgtga taatcgctgg aatcttcagt cggttccgcg ttatcagtcc
    10861 atttgttttc ttccgccata cgacgtgctt cttcagcaac gcgacgtgct tcttcttcga
    10921 gtttacgacg cgcttcttct tcggctttac gcttgagctc tgcagcttcc tgctcacggc
    10981 gggctttttc agcctgggcg tttttagtca tatcgtcttg ttgattgctc actttgtctt
    11041 tttccgcagc ttcacgtttc gcttgttcag cagcttcacg cttagcttgt tctgcagcct
    11101 cacgttcagc tttttgttgc gcctcgcgtt tagccgattc ttctgcctca cgacgggctt
    11161 gctcttccgc ttcacgctgc gcttgctctt ccgctgcaag gcgttcagcc tcttgcggat
    11221 cgcgtttcac aaaggtgcgt ttcttgcgga cttcgatttg taccgatttg ctttttccac
    11281 cggtaccagg aatgttaagg gtgctgcgtg ttttacgttg cagcgtcaat ttgtccgggc
    11341 ctgaattttt ctgattcagg tggtcaatca aagtctgttt ctcttgtgcg gacacagagt
    11401 cgtcagcaga cttccggata cctgcatcag caaattgctg taccaggcgt tccacggagg
    11461 tctgtcgctc tgcggccagc gttttaatcg ttacatctgt catgctgttc cttcctgcta
    11521 cagtttatta cgcttcgtca ccgaaccagc aaatattacg ggcagccata atcagtgctc
    11581 cggctttttc gtcggtcaac ccttcgatat cagccagatc atcaatgccc tgttcggcga
    11641 gatcttccag cgtacaaacg ccacgggcgg ccagtttgaa tgccaaatca cgatctatcc
    11701 cttcaaggtt cagcagatcg tcagccggtt tgttatcacc gaggctttct tcctgggcct
    11761 gtgcaatggt ggccagtgca tttttagcac gctcgcgcag tgcttcaacg gtcggctcat
    11821 caaggccttc gatttccaac agctctttca tcggcacata ggccagttct tccagcgtcg
    11881 agaagccttc ttctaccaga acagtcgcga agtcttcgtc gatgtcgaga tatttggtga
    11941 aggtgtcgat cgctgcgtgc gcttccgcct gatgcttagc ctgcaggtcg tcaacggtca
    12001 tcacgttgag ttcccaaccg ctcagctgcg aagccagacg cacgttctga ccgttacggc
    12061 caatcgcctg cgccaggtta ccggcttcaa cggcgatatc catggtgtgt ttatcttcat
    12121 ccaccacgat agaagcaacg tctgccggtg ccattgcgtt aatcacaaac tgcgccgggt
    12181 tatcatccca caggacgata tcgatacgct cgccgcccag ttcagtagaa accgcctgaa
    12241 cacgcgcgcc acgcatacct acacaggcac ctaccggatc gatacgttta tcgttggttt
    12301 tcaccgcgat tttcgcacga gaacccggat cgcgagccgc tgctttaatt tcaatcactt
    12361 cttcgccgat ttctggcact tcaatacgga acagttcgat cagcatttcc ggcttggaac
    12421 gagtgacgaa cagttgcgcg ccacgcgctt ccgggcgaac ggaatagagc acgccacgaa
    12481 cgcggtcgcc agggcggaag ttttcacgcg gcagcatatc ttcgcgcagg atcacggctt
    12541 cagcattgtt gcccagatcc agagagatgt tgtcgcggtt tacttttttc accacgccgg
    12601 tgatgatttc accttcgtgt tcacggaact gatcaaccac catcgcacgt tcggcttcac
    12661 gcactttctg cacgataacc tgttttgccg tctgggtagt gatacggtca aaggtaacag
    12721 actcaatctg atcttcaacg taatcgccca ggttcaggct ttcatcttca taacgtgcgg
    12781 cttcaagggt gatttccttg gtcggctggg tgacttcatc aacaactaac cagcgacgga
    12841 aggtgtcaaa atcaccgctt ttgcgatcga tctgtacgcg gacgtcgatc tcttgttcat
    12901 attttttctt tgttgctgtc gccagcgcgc tttccaatgc ttcgaaaatc ttctcgcgag
    12961 gtagcgcctt ttcattggat acggcttcaa ctacagccaa aatttctttg ttcatcgcgg
    13021 gcttttcacc tcatccagac tattaaaagt ggggaaccag gttcgccttc tggatattac
    13081 tcagcgcgaa cacttcatct ttaccttcga cggtaactgt gatcatttca ccgtctaccg
    13141 ctttgataac gccctgccat ttacgacggt tttgtaccgc catacggaga accagagtca
    13201 cctcttctcc gacaaaacgg gcgtagtgtt cagccgtgaa cagtgggcga tcgagacccg
    13261 gtgaggagac ttccaggtta taagcaacgg tgatgggatc ttcaacatcc agcacagcac
    13321 ttacctggtg gctcacatca gcacaatcat caacattgat gccatcttca ctatcaatat
    13381 agatgcgcag tgtggatgtg cgaccgcgaa taaattcgat gccaaccagt tcaaaaccca
    13441 gggcctcaac tggcgcagta atcatctctg ttaatttttg ctctaatgtg gacaagccca
    13501 cccccaagac ataaaaaaag ggcctaaagc ccagttattc tgtagtcaga taacaaaaaa
    13561 ccccgataaa tcggggcttt atataactga accctataac cgcaactgcg gtctggagca
    13621 ctttccagaa ggattttttc aaatcccact acgaaggccg aagtcttcac agtatatttg
    13681 aaaaaggact ctaagggaaa gtggttgcgg gggccggatt tgaaccgacg accttcgggt
    13741 tatgagcccg acgagctacc aggctgctcc accccgcgcc tgaaacgtgg caaattctac
    13801 tcgttttggg taaaaaatgc aaatactgct gggatttggt gtaccgagac gggacgtaaa
    13861 atctgcaggc attatagtga tccacgccac attttgtcaa cgtttattgc taatcatgtg
    13921 aatgaatatc cagttcactt tcatttgttg aatacttttg ccttctcctg ctccccctta
    13981 agcgcattat tttacaaaaa acacactaaa ctcttcctgt ctccgataaa agatgattaa
    14041 atgaaaactc atttattttg cataaaaatt cagtgagagc ggaaatccag gctcatcatc
    14101 agttaattaa gcagggtgtt attttatgac gacgattctc aagcatctcc cggtaggtca
    14161 acgtattggt atcgcttttt ccggcggtct ggacaccagt gccgcactgc tgtggatgcg
    14221 acaaaaggga gcggttcctt atgcatatac tgcaaacctg ggccagccag acgaagagga
    14281 ttatgatgcg atccctcgtc gtgccatgga atacggcgcg gagaacgcac gtctgatcga
    14341 ctgccgcaaa caactggtgg ccgaaggtat tgccgctatt cagtgtggcg catttcataa
    14401 caccaccggc ggcctgacct atttcaacac gacgccgctg ggccgcgccg tgactggtac
    14461 catgctggtt gctgcgatga aagaagatgg cgtgaatatc tggggtgacg gtagcaccta
    14521 taaaggaaac gatatcgaac gtttctatcg ttatggtctg ctgaccaatg ctgaactgca
    14581 gatttacaaa ccgtggcttg atactgactt tattgatgaa ctgggcggcc gtcatgagat
    14641 gtctgaattt atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa aagcctactc
    14701 cacagactcc aacatgcttg gtgcaacgca cgaagcgaag gatctggaat acctcaactc
    14761 cagcgtcaaa atcgtcaacc caattatggg cgtgaaattc tgggatgaga gcgtgaagat
    14821 cccggcagaa gaagtcacag tacgctttga gcaaggtcat ccggtagcgc tgaacggtaa
    14881 aacctttagc gacgacgtag aaatgatgct ggaagctaac cgcatcggcg gtcgtcacgg
    14941 cctgggcatg agcgaccaga ttgaaaaccg tatcatcgaa gcgaaaagcc gtggtattta
    15001 cgaggctccg gggatggcac tgctgcacat tgcgtatgaa cgtctgttga ccggtattca
    15061 caacgaagac accattgagc agtatcatgc acatggtcgt cagttgggcc gtctgctgta
    15121 ccaggggcgt tggtttgact cccaggcgct gatgctgcgt gactctctgc aacgctgggt
    15181 tgccagccag atcactggtg aagttaccct ggagctgcgc cgtgggaacg attattcaat
    15241 cctgaatacc gtctcagaga acctgaccta caagccagag cgtctgacga tggaaaaagg
    15301 cgactcggtg ttctcgccag atgatcgtat tggtcaattg accatgcgta atctggatat
    15361 cactgatacc cgagagaaac tttttggtta tgccaaaact ggcctgctct cctcctctgc
    15421 cacttcaggc gtgccgcagg tggagaatat ggaaaacaaa ggccagtaat tcgacatcaa
    15481 ccctgccctt caggggtggg gttaccttgc ttatcctctg taaactcctt tctgagcatc
    15541 acataatcga aaacatcatg gtttgcatcc acaacccgtg agtctccttt atagtgtgca
    15601 atcacttcct caagcgtttg cggctgttcc ggctttgtta cccccatcca cgcattaata
    15661 aggtaattat tgtaagctgt cgaaaagatg ttgttttccg ttgtgcgatc cacgccatcc
    15721 ccaagaacgg ggctatacca gataaacatc gggacatgat atgcctgctg gctagcctcc
    15781 ctgcctccat gaaaatagac gttcttcttc gtagggtcac gttccaggcc gtggtcggca
    15841 aaatacatga ccgaggcgcg gcgatctttt aataattcaa aaacctgacc tagcaaacta
    15901 tctgtgtaat gaatggagtt gtcatagcag gcatcctgat cgtcctgcgg ttgaaacacg
    15961 gcgctggatt gcggataggc gctacaagcc ggttcatggc ttccatttaa atgaagaaca
    16021 atcagtttct tttgctgcgt attttgctgt aatgcttgcg ataaatgcgg caacaacaat
    16081 tcatcaaatc ctctgacata aactgtttcc atggcgcgca tggcgatact ggtaactgct
    16141 gtaccattct gccgaaaagc ggattgcgag cttagccaga aagtctgaaa tcctgcttga
    16201 ttagccatat taataatgtt gtcggggtaa ttatgaatgt catgactcaa aacagaatca
    16261 gcagttaaag aaaggggaac cgacagcgca gtgtaaggtg cgccgcttat tgcttgatta
    16321 aacagtttga tctgttttct ttgtgcttca acttgcggtg tcgtagagcg tgtatatcca
    16381 tacaaagaca tattgtcgac acgtacagac tcccccacaa tcaacacgta ggtatcaata
    16441 cctgtatccc tgactgataa ttgaaaatac ggcaccgtgt ttgcaattga gagtaatctt
    16501 tgatgctctt tcgctgctaa agcaaaatag ttgagattga aaaacggcgt ataggtagca
    16561 aatcgcgacg ctagtatata tggactgaac gcttttttat tttttgcatc tttataagca
    16621 aattgacaag cggaaaataa actgcccgaa atgacaatca gcaataatat tcctgtcact
    16681 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
    16741 aagcaaaggc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
    16801 cactctggag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
    16861 aatagaggct aatactcatg tctgccgtag tcagtaacac aaatggtgcg gcaacaagca
    16921 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
    16981 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
    17041 cacaaagata caacaaccaa tgagatttaa aacttctagc aaatttgttg aatactgtca
    17101 tccagaaatc caagttcaca accaatataa acaattatca acaatgttca attttatatc
    17161 agacatgttt ttcaactctg atccatatca gaccgaaaca ctgttagttt tttcttttta
    17221 aatgatgcgg ccattgcgtt ctcagtaatt aaataacatt atcaatgcgt ctctaaacat
    17281 tacgcagaac gtagccatca gtagttatac tgacaggagc aggatggttt atcgagaagt
    17341 aaacatatcc gtattcgtac cggaactgga gttgacacaa taaagtgcca attatgtcag
    17401 cagaagggaa aagtttataa ctaaggcgta ccggcaccat cgtttcaagg taccagctac
    17461 gagtaaagca actggacgag atacagatac ctgacaacca ttcctcagac caggaccaaa
    17521 acgaaaaaag acgcttttca gcgtctcttt tctggaattt ggtaccgagg acgggacttg
    17581 aacccgtaag ccctattggg cactaccacc tcaaggtagc gtgtctacca attccaccac
    17641 ctcggcacgg atactacttt ttagttcggg atatcgctgg tcggcttagc cggagcagct
    17701 ggttgagttt gttcggtttt caccggtgca ctcagatttt cccattcgct acctttattg
    17761 gttttgttgc tattgatgtt acccagcacc agactgatga tgaagaataa cgttgccagc
    17821 agcgccgtca tgcgggtcat gaagttacca gaaccacttg aaccaaacag cgtagcggaa
    17881 gcgcctgctc cgaaggaggc tcccatatca gcgcctttac cttgctgcag catgatcaga
    17941 ccaacaaggc caattgccac aataaggaaa actactaaaa gagcttcgta cataatcaac
    18001 ctgttccttg cggagttgcc gcgtaccaat gcttcaacca ataaagcggg agtttttaat
    18061 ctttcccact gaagcgggtg tgaatactaa ccaaagcgaa tgaccttcgc aagggcaatt
    18121 ttatcgcatt gtatcaactg cggaaaaaaa cagcaaaacc gatgtgttcg ctgagaaaaa
    18181 aggcgacaat cgccgccttt ttagccagtt atctaacgct ttaaacggct tttactgcat
    18241 cggcgatgcg gtgtgcaaat tcagtcacct gcgcttcgtc ttcgccttcc accatcacgc
    18301 gaattaacgg ttcggtgccg gatttacgca gcaacacgcg tccacggttg cccagcgcag
    18361 cttcaacctc tgcggtcacg gctttaactg actcatgctc aagtggatcg ccgctacctg
    18421 cggtgtaacg tacgttaacc agaatctgcg ggaacatttt catgccgctg caaaggtcgt
    18481 gcaggctcat atggttacgt gccatcgccg ccagcacctg caagccagca acgatgccgt
    18541 caccggtagt agttttatcc agtaggatca catgaccgga attctctgca ccgatacgcc
    18601 agcctttctc ctgcattttt tccagtacgt agcggtcacc cactttcgcg cgcgcaaatg
    18661 gaattcctaa ctgtttcagc gccagttcaa gccccatgtt gctcatcaat gtacccacag
    18721 cgccaccacg cagctggccc tgacgaagac cttcacgcgc gatgatatac atgatctgat
    18781 cgccatcgac tttattgcct tcatggtcga ccataatcac gcgatcgcca tcgccgtcga
    18841 aggcaatacc gagatccgct ttttcagcca gcacacgagc ctggagcgcg cgaacgtcgg
    18901 tagcccccac ttcggcattg atgtttacac cgtttggctc acaaccgata gcgataacgt
    18961 tcgcccccag ttcgcgcagc acgttcggcg cgatgtgata agtcgcaccg tttgcacaat
    19021 ccaccacaat cttcagttca ctgaggctaa gttcgttcgg gaacgtggct ttgcaaaact
    19081 cgatatagcg acccgcggca tcaacgatac ggctggcttt acccagttct gccgaatcaa
    19141 cgcagctgat ctccttttcc atttccgctt cgatggcctc ttctaccgca tccggcagtt
    19201 tggtgccgtc gatagagaag aatttaatcc cattatcgta gaacgggtta tgcgatgcag
    19261 atatcacaat tccggcctct gcgcggaagg tacgcgtcag ataagccacg gccggtgttg
    19321 gcatcgggcc agtgaagagt gcggaaaggc ccgctgccgc cagacccgct tccagtgctg
    19381 actccagcat atagccagaa atacgcgtgt ctttaccaat aataatctta cgggagccgt
    19441 ggcgcgccag cactttaccc gcggcccaac ccagcttaag cacaaaatca ggtgtgatcg
    19501 gcgcatcccc tacacgacca cgaatcccat cggtaccgaa atatttacga ttactcatag
    19561 cgtttgtttt cctttgcaga cagagtggct tccaccaccc gcatcgcttc tacggtttct
    19621 ttgacgtcat gaacacgaat gatgtgcgcg ccttgcattg cggcaatgac cgcacaggcc
    19681 agactaccgc tcaggcgctc ggacggcccc acgttcagca gctgcccaat catcgatttt
    19741 cgtgacatac ccaccaacag cggcaggttg aaatggtgaa attcagccag gcgcgccagt
    19801 aatgaatagt tatgggagag atttttaccg aaaccgaatc cggggtcgag caacaatttc
    19861 tcttttgcga tacccgcctg ctcgcaacgt gctatttgct caataaagta gcgattcact
    19921 tctgcaaaga catcgtcata cttcggagct tcctgcatgg tttttggatt tccctgcata
    19981 tgcatcagac aaaccggtaa accggtttct gcagccgcct ccagagcgcc aggttcggaa
    20041 agggagcgga tatcattaat aatgtgagcg ccaactttcg ctgactcacg gatgacttct
    20101 ggtttggatg tatcgaccga gatccagact tcgaagcgtt gagcaattgc ctcaaccaca
    20161 ggaataacac gttgcaactc ttcttcaacg ctaacttccg ccgcccctgg gcgcgtggac
    20221 tcgccaccaa cgtcaatgat cgtcgcgcca gcattgatca tcagattcgc atgtttcacc
    20281 gcatctatca gcgagttatg cgtgccgcca tccgaaaagg aatcaggcgt gacgttgagg
    20341 atccccatta cgtgaggatg gctaaggtcc agtgaagtac cctgggcaaa gagtttcatg
    20401 atgttatccc tggtatgaat tgataagaaa aaaccccgga gcacgccccg gggttttcgg
    20461 tacaaataca gtcatctgat gcgggaactt acttgtcgcc taactgctct gacatggtgt
    20521 tacccgggtt cggcgtacgc ggttcatcaa ccggacgagg agcctttgga ctaccattgt
    20581 cgccagaatt gttagaagcg cctggttctt cccagcccgc tggcggacgt acatcgcgac
    20641 gtgccatcag gtcatcaatc tgcggtgcgt cgatagtctc atatttcatg agagcatctt
    20701 tcatcgcatg cagaatatcc atattgtcgg tcagaagctg acgcgcacga ttatagttac
    20761 gctcaatcag tgctttcact tcctggtcga tgatacgtgc agtttcatcg gacatatgtt
    20821 tcgctttcgc tacgctacgg ccgaggaata cttcaccttc ttcttccgcg tacagcagtg
    20881 gacccaattt ctcagagaag ccccactggg tcaccatgtt acgtgccaga ttggtcgcaa
    20941 ctttaatatc gttggacgca ccggtagaaa catgttccgg cccatagatg atctcttctg
    21001 ccagacgacc accgtacagc gtagaaatct ggctttccag tttctgacgg ctggcgctga
    21061 ttgcgtcgcc ctcaggcaag aagaaagtca cacccagcgc acgaccgcgt gggataatcg
    21121 tcactttgtg caccggatcg tgttccggca ccaggcgacc gataatcgca tgacccgctt
    21181 cgtggtaagc cgttgattct ttctgcgctt ccgtcatcac catggagcga cgttccgcac
    21241 ccatcatgat tttgtctttc gctttctcga actcaaccat cgacacaacg cgtttgttgc
    21301 cacgagcagc gaacagtgcc gcttcgttca ccaggttcgc caggtcagca ccggagaaac
    21361 caggagtacc acgggcaatg attgccgcgt cgatatcggg ggccaatggt acgcgacgca
    21421 tgtgaacttt caggatctgc tcacgaccgc gaacatctgg caagccgacc acaacctgac
    21481 ggtcgaaacg gccaggacgc agcagggccg ggtcgagaac gtccggacgg ttagtcgcgg
    21541 cgataacgat gataccttcg ttaccttcga agccatccat ctcaaccagc atctggttca
    21601 gagtctgttc acgttcatcg tgaccaccgc ccagaccagc gccacgctgg cggcctacgg
    21661 cgtcgatttc atcgataaag atgatgcacg gtgccgcttt cttcgcctgt tcgaacatgt
    21721 cacgaacacg ggatgcaccc acaccgacga acatttctac gaagtcagaa ccggagatag
    21781 taaagaacgg aactttcgct tcgcctgcaa tcgctttcgc cagcaacgtt ttaccggtac
    21841 ccggaggacc gaccatcagg acgcctttcg ggatcttacc gccgagtttc tggaagcggc
    21901 tcggctcgcg cagatactct accagttcag caacttcttc ttttgcttcg tcgcagcccg
    21961 caacgtcagc aaaggtcgtt ttgatctgat cttccgtcag catgcgcgct ttgctcttac
    22021 caaacgacat ggcacctttg ccaccgccgc cctgcatttg acgcatgaag aagatccaga
    22081 caccaatcag caacagcatc gggaaccagg agatgaagat agaagccagc aggcttggtt
    22141 cttcaggcgg ttcaccgaca accttgacgt tcttggtcaa caggttatcc agtaatttcg
    22201 gatcctgaac cggaatgtaa gtggtataac ggttactatc tttcttggta acgttgattt
    22261 cacgtccgtt gatacgcgct tcacgaacct ggtcgttatt gacctcttgt aggaaggtag
    22321 agtaatccac cttacggcca ttagactcgc tgggcccaaa gctctggaat actgacatca
    22381 gcacaacggc aatgaccagc cagagtatta ggtttttcgc catgtcactc aagggattaa
    22441 cctcttatta caactgtgtt aaaaacagcg tcaggatact ctatatccag catctttcaa
    22501 actttcgtct gaaatctccc ggttagggtt tacgcccggt cgctacaata tacacttccc
    22561 gcgaacgtgc acgagaagag tccggcttac gaactttgac cttcgtaaac agggagcgaa
    22621 tttcccttag atactcatcg aaaccttcgc cctggaacac cttcactaca aaactgccac
    22681 ctggcgctaa tacatcacga cacatttcta gcgccagttc caccagatac atggcacggg
    22741 ggatatccac cgccggtgtt ccgctcatgt ttggtgccat atcggacatg acaacctgga
    22801 ctttgctgtc gccaacgcgc tccagcagtg ctttcatcac cagttcatca cgaaaatcgc
    22861 cctgaagaaa gtccacacca acgataggat ccataggtaa aagatcgcaa gcgatgatgc
    22921 ggcctttgcc gccaatttgg gtgaccacat attgtgacca accacccgga gcagcaccaa
    22981 ggtcgacaac cgtcattccc ggtttaaaga gtttgtcact ttgctgtatt tcatcaagtt
    23041 taaaccaggc acgggaacgt aacccctttt tctgtgcctg ttgaacatat ttatcgctaa
    23101 agtgttcctg aagccagcgg ctggagctgg cagaacgctt cttacctgtc atttaacttt
    23161 cccatgggga taactcatcg taaccaattg cgtaaatttt tacgcgccta tttggtgata
    23221 tatgggagat ggcggtagaa tgacccgttt tcaatcccaa cgtaagcaaa aaatacgatg
    23281 aatctgagta ctaaacaaaa acagcacctg aaaggtctgg cacatccgct caagccagtt
    23341 gttctgcttg gcagtaatgg tttgaccgaa ggggtgctgg ccgagattga acaagcgtta
    23401 gagcaccatg aactcatcaa ggtgaaaatc gccaccgaag atcgcgaaac taaaaccttg
    23461 atcgtggaag ctatcgtgcg cgaaaccggc gcctgtaatg tacaggtcat cggtaaaacg
    23521 ctggtgcttt atcgcccaac taaagaacgt aaaatctcgc tgccacgcta agattatcct
    23581 aaagttacac acatttgctg tgtaaaacga ggggttttcc gcaggcagga gagcaaaatg
    23641 ccacgctctg ttcgttgata aaaggccgca tagcggcctt tttcctttct ttacaataca
    23701 tcaacatctt gagtattggg taattcttac aggtattcca ccttaattac ttcaaattct
    23761 acttcgccgc ccggcgtttt gatgaccaca acatcatctt cttctttgcc gatcaggcca
    23821 cgagcaatag gcgagtttac agaaatcagg ttttgtttaa agtcagcttc gtcatcgcca
    23881 acgatgcgat aagtctgttc ttcgtcagaa tccagattca gcaccgttac ggtagcacca
    23941 aaaataacgc gcccattgtt gggcattttg gtgacatcaa tcacctgcgc gttcgacagc
    24001 ttggcttcga tgtctttaat acggccttcg cagaaaccct gctgttcacg agctgcgtgg
    24061 tattcggcgt tttctttcag gtcgccatgc tcacgcgctt ccgcgatagc agcaatgatt
    24121 tcaggacggc gcacagattt cagaaaatcc agctcttcgc gtaatttttc agcgccgcgt
    24181 aaggtcatcg gaatagcttg catttgttat acctcttgaa tattcctgat ggggcaagtc
    24241 ttcacccacc ccggctgttc ggcctgcccg gcataactcc tcaccgggac cagaagcaaa
    24301 aaaataccga cccgggtaca agtcccaggt cagctacaat tcacattttg atagtcattt
    24361 taccctgaag ttcccgaagg gtcatcgttt actttatagg gcgttgcgcc gtagtatgac
    24421 ggctcgattc caggttgtta gcgcgagatt atgcgatttt ccagatttat catcggattg
    24481 accagctgta tagcgttcag tgttcaggcc gcgaatgttg atgagtacgt tactcaactc
    24541 cccgctggtg ccaaccttgc cctgatggtg caaaaagtcg gcgcgtcggc ccccgctatt
    24601 gattaccaca gtcagcagat ggcgctgccc gccagtacgc agaaagtgat tactgcgctg
    24661 gcggcgttga ttcaactcgg ccccgatttt cgtttcacca cgacgcttga aaccaaaggc
    24721 aatgtggaaa acggcgtact taagggtgac ttagtggcgc gatttggtgc cgatccgacg
    24781 ttaaaacgtc aggatattcg caatatggtc gcgactttga aaaaatctgg cgtcaaccaa
    24841 atcgatggca atgtgttgat agatacctcc attttcgcca gccacgataa agcccccggc
    24901 tggccatgga atgacatgac acaatgcttt agcgctccgc ctgccgctgc catagttgac
    24961 cgcaactgtt tctccgtctc gctctacagt gccccaaaac ctggtgatat ggcttttata
    25021 cgcgtggcat cttattaccc cgttacgatg ttcagccagg tacgcaccct cccccgaggt
    25081 tctgccgaag cgcaatactg cgaactggat gtcgtaccag gcgacctgaa ccgctttacg
    25141 ctgacgggat gcctgccaca acgttctgag ccgctcccgt tggcttttgc cgtgcaggat
    25201 ggagccagct atgccggggc aattctgaaa gatgagttaa aacaggcagg tatcacctgg
    25261 agcggaacac tgctgcgcca gactcaggtt aacgaacctg gaacggtagt tgccagtaaa
    25321 cagtcggccc cgttgcacga tctgcttaag attatgctga aaaagtcgga caacatgatc
    25381 gccgatacgg ttttccgcat gataggccat gcgcgcttca atgtgcctgg aacatggcgg
    25441 gccgggtcgg acgccgtgcg tcagatcctg cgccagcaag ccggtgtcga tattggaaac
    25501 accattattg ccgatggttc agggctttcg cggcataacc tgattgcccc cgccaccatg
    25561 atgcaggtgt tgcaatacat tgcccaacac gacaatgaac ttaactttat ctccatgctg
    25621 ccgctggcgg gctatgacgg ctctttgcag taccgtgcag gtctgcatca ggcgggcgtg
    25681 gatggaaaag tctcagcgaa aaccggttcg ttgcaggggg tatataacct ggcgggattc
    25741 attaccacag cgagcgggca acgaatggcg tttgtacaat atctttctgg ctatgcagta
    25801 gaaccggcgg atcagcgtaa tcgccgtatt ccgttagtgc gttttgaaag ccgtttgtat
    25861 aaagatattt atcagaacaa ttagtcaaaa agaaaccccg gcacatggcc ggggttttgc
    25921 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcatcatc
    25981 ccagtcctca tcatcctctt cagcaatctc ttcaagctgc tggcgatgat aatcatccca
    26041 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    26101 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccactcg ccgcagagat
    26161 cagataatat ttatcttccc agcccagcgc ttcagcaatc gctttcgctt tctcttcagc
    26221 ttctgcctta tccagcagat cgatcttatt gaacactaac caacgcggtt tcgccgccag
    26281 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    26341 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    26401 caggaagcgg atacccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    26461 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    26521 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcgcgaa taaaggtcga
    26581 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    26641 cagctcacgc ttatcacccg gcgtaccgtt ggttttctgc cgtggtgtac ggttaacgga
    26701 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    26761 accgtgttta gtcatatcgc ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    26821 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    26881 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    26941 gttttcgtcg gcttccatcc atacgtcacc accatcacca ccgtcgccgc catccgggcc
    27001 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    27061 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    27121 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    27181 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    27241 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    27301 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    27361 cgcttccgcc agggcgccat atcctaccaa tgtattcagt ccgcaaaaaa ttaaacatgc
    27421 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcaat
    27481 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    27541 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    27601 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    27661 caggccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    27721 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    27781 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    27841 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    27901 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    27961 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    28021 ccagcaaatt gctgtggtga gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    28081 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    28141 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    28201 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    28261 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    28321 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    28381 accgaaacgc ttaacgccca ggcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    28441 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    28501 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    28561 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    28621 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    28681 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    28741 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    28801 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    28861 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    28921 aaaacacaac ttgtacggta acgtttatct gtgccatttt ttcagtacaa tcactctata
    28981 ttcctaacca taaaccctaa gttgcctttg ttcacagtaa ggtaatcggg gcgaaaagcc
    29041 cggcttttgc gatgaattta gaaaaaatca atgagttaac cgcgcaagat atggcgggtg
    29101 ttaatgcggc aatccttgag cagcttaatt ccgacgtcca gctgatcaat cagttaggct
    29161 attacatcgt cagcggcggc ggtaaacgta ttcgtccgat gattgctgta ctggctgcac
    29221 gagctgttgg ctatgaggga aatgcgcatg tcaccattgc tgccctgatc gagtttatcc
    29281 acacggcgac tctgctgcac gacgacgttg tggatgaatc agatatgcgc aggggtaaag
    29341 ctaccgccaa cgctgcattt ggcaatgccg ccagcgtgct ggtaggcgat tttatttata
    29401 cccgcgcgtt ccagatgatg accagcctcg gttcgctcaa agtgctggaa gtcatgtcag
    29461 aagccgtaaa cgtcatcgca gaaggtgaag ttctgcaact gatgaacgtt aacgatccgg
    29521 acatcactga agaaaactac atgcgcgtta tctatagcaa aaccgcgcgt ctgtttgagg
    29581 ctgccgcgca gtgttccggg attctggctg gctgtacgcc ggaagaggag aaaggcctgc
    29641 aggattatgg gcgctatctc ggcactgctt tccagttgat cgacgattta ctcgattaca
    29701 atgccgatgg cgaacagtta ggtaaaaatg tcggcgacga tctgaacgaa ggtaaaccga
    29761 cgctgccgct gctgcatgcg atgcatcatg gcacaccaga acaggcacag atgatccgta
    29821 ccgccatcga acagggtaac ggtcgccatc ttctggaacc ggttctggaa gcaatgaacg
    29881 cttgtggatc tcttgaatgg acgcgtcagc gtgccgagga agaagcagac aaagccatcg
    29941 cagcgttaca ggtgctcccg gacacccctt ggcgagaagc actcatcggc ctcgcgcaca
    30001 tcgctgttca acgcgatcgt taatcccctc ccctcatccc gcgcagcgcg cggggtggtt
    30061 ccagtaaatt ccataaaaac acttactcag ctctataaca cctgaaaaac aggctgtgca
    30121 tctttatata ttctgaatat tcacactcct tacaggaact ttttagagca atcggccatc
    30181 aggagtatag tgatgctcga cagaagaagt gttctgaatg aaagcgacta acttaaggag
    30241 tgaggaaaga tggaaagtaa tttcattgac tggcatcccg ctgacatcat tgcgggtttg
    30301 cgcaagaagg gaacgtcaat ggcggcggag tctcgcagaa atggtttgag ttcctcaacg
    30361 ctggcgaatg cattatcgcg cccatggccg aaaggagaga tgattattgc gaaagccctg
    30421 ggaactgacc cctgggttat ctggccatca cgctaccatg atccgcagac ccatgagttt
    30481 atcgacagaa cgcagttgat gcgtagctac actaaaccga aaaaatgagt ggtctggcgg
    30541 tagccccgcg aacggggctg ccagctctca gacgattatt cgcctttcac acgctcaata
    30601 tttgcaccta aagcgcgcag tttgtcttca atgcgttcgt agccacgatc gatgtgataa
    30661 atacgatcaa ccaccgtcgt cccttccgca atacatccag ccagcaccag gcttgctgat
    30721 gcacgcagat cggttgccat aacctgtgcg ccagaaagtt tttcaacacc gtgacaaata
    30781 acggtattgc tttcgatttc ggcatgcgcg cccatacggc taagctccgg cacatgcata
    30841 aagcggtttt caaagaccgt ttcggtgatg aatccggtcc cttctgccac caggttcaac
    30901 agcgtgaact gggcctgcat atcggtcggg aatgccggat gcggcgcggt acgtacgtta
    30961 acagccttcg gacgtttgcc atgcatatcc aggctaatcc agtcttcgcc gacttcgatg
    31021 tccgctccag cgtcacgcag tttcgccagc acggcgtcga gagtatctgg ctgcgcgtta
    31081 cggcagataa ttttgccgcg agaaatcgcc gccgccacca ggaaagtacc ggtttcgata
    31141 cgatccggta gcacgcgata gacaccgccg cctaaacgtt ccacaccttc gatgacgata
    31201 cgatcggtgc cctgaccgct aattttcgca cccagcgtaa tcaggaagtt cgcggtatcg
    31261 acgatttccg gttcacgcgc tgcgttttca ataatcgtgg taccttccgc cagggttgca
    31321 gcacacatga tggtcaccgt tgcgccaacg ctgactttat ccatcacgat atgtgcgcct
    31381 ttcagacgac catcgacgga agctttaacg taaccttctt ccagtttgat ggtcgcgcct
    31441 aattgttcga ggccagaaat gtgtagatca accggacgcg caccgatcgt acaaccgcca
    31501 ggcagtgaaa cttgcccctg accaaagcgc gctaccagcg gccccagtgc ccagatagaa
    31561 gcacgcatgg ttttaaccag atcgtaaggt gcgcagaata cattaacgtc gcgggcatca
    31621 atatgcacag aaccattacg ttctactttc gcacccagct ggcttagcag cttcattgat
    31681 gtatcgacgt ctttcagttt tgggacgttc tggatctcta ccggttcttc cgccagtaga
    31741 gcggcaaaaa ggataggcag agcagcattt ttagcgccgg aaattgtgac ttcgccctgg
    31801 agcttcgttg gcccctgaac acgaaattta tccatttagt ttgttctcag ttaacaattc
    31861 atatccgcta ccggcgaatc gcccatagct caaaagccgt tcagtttgcg atcgcgcgcc
    31921 cactccgcag gggtatacgc tttgatcgac acagcatgaa tgcggttatc cgcaatatat
    31981 tccatcagcg ggccatagac cgtctgctgt tttttaaccc gactcatgcc gtcaaacaac
    32041 tcacccacgg caataacctg aaagtggctg ccatcgccgg aaacgtggac ttcctggagg
    32101 gagagagcgt tcatcaacac gctctgaatt tcattatttt ccatgggatc ttcaatcatc
    32161 agttaataaa ccagcgaaac atcttagagc aaagttgcgc tggcataaat aagcaaaaag
    32221 cctcgctgat aaatcagaca aggctcgact tgcaggcagg tttgccggac aggcggttaa
    32281 cgccatatcc ggcctgaaaa aatttaacga ggcagaacat cagcaggcaa attatacaat
    32341 ttcgccaggg tatacacttt gtcgtttacc ccctgaagcg tcacattgtt gccctgcttt
    32401 ttcgccagat cgataagatg gagcagcagt gccagtcccc ccgtatccac gcgggacaca
    32461 cggctaagat cgatgcaggt aatccccttc accgcttcct cacgcatttc ccaaagcggt
    32521 agcaaaacgt cctgatccag ctctccggat aacgccagcg tgtcacccgt ctgcatccag
    32581 ctcagtgact cgctcattat tttttctctt ccagagtgat tttctgttga gaaatcgatt
    32641 tcagttgcgc agtcaggccg tcgatacctt tggtacgcag cagcgttccc cactcgtttt
    32701 gtttggtggt gatcatactg acgccttcag caatcatgtc gtaagcctgc cagttgcccg
    32761 tctgggagtt tttacgccac tggaagtcca gacgcaccgg cgggcggcca ttcggatcaa
    32821 taatggtaac gcgaatcggc acaatggttt tatcgcccag cggctgttct ggcgcaatct
    32881 gataggtttg accgtgatac atcgccagcg cctgaccgta agcctgcttc aggtactcac
    32941 ggaaagcggc aaagtaggct tcacgttgag caggggtcgc actcttgtaa tactggccca
    33001 gcaccagcgc accggcgtat ttcacctgta cgtatggcag cagttcctga tcaacaatgg
    33061 tacgcagata atccgggttg gcccgaattt gcggttgctc attcttcagg cgatcgaacg
    33121 ttttctgcgc cgcctcgtcc atcagcttat aaggattggt ctggtctgcc gcggttgccg
    33181 cactcagagg tgcaatcacc agcaaagcga ccatcattaa acgtttaaac atgcgtcggt
    33241 tctcctgaaa ttatttcgtt gtacccacag gttcagtggt ttcattatta cctggcgcag
    33301 cagctggcgc atcgccacta ttcttattgt catcgccttt actaccgtaa aggaactgac
    33361 caatgagatc ttccagcacc atcgcagact tagtgtcctg aattgtatcg ccatccttca
    33421 ggatagcagt ccccagttcc gggtcttcaa aaccgacgtt taatgccaga tattgttccc
    33481 ccagcaggcc ggaagtacga atgctcagcg aactggtatc tggaatgtgg ttataacgtt
    33541 gttcaatttc cagcgttacg cgcggcagat aggttttcgg gtccagcgta atatccgcca
    33601 cccgacccac aacaacgcca ccaatactga ccggagagcg cgctttcagg ccgccaatgt
    33661 tatcgaacgt cgcataaagc gtgtaggtcg gttcagtacg tatggacgtc acgttcgccg
    33721 ccttcaggca aacaaacagc gccgccagca gtgctgctaa taaaaagata cccacccaaa
    33781 tttcattttt tttcgtttgc atgaactcaa ttcccaaaca tcaatgcggt cagcacaaaa
    33841 tccagcccca gaacagccag agacgagtgg acaacggtgc gagtggttgc ccggctaatc
    33901 ccggcagacg tcgggatggc gtcgtagccg ttaaacaacg aaatccacgt caccgtgatg
    33961 gcgaacacca cgctcttaat cagacagttg accagatcca tacgccagtc gacggcattt
    34021 tgcattgccg accagaagaa cccgctatca atgcctttcc aactgacgcc gaccagagat
    34081 ccgccccaga tccccacggc gacgaaaata accgtcaaca gtggtaatga aataacccca
    34141 gcccagaaac ggggagaaat aacccgacgc agcggatcca ccgccatcat ctccatactg
    34201 gagagttgct ctgtagcgcg catcaggccg atttctgcgg ttagcgccga accagcacgc
    34261 ccggcaaaca acaacgcggc aaccaccggc cccagttcac gcagtagcga taacgccacc
    34321 agcataccca gactggtttc cgcactataa gtggtcagaa ccagatagcc ttgcagcccc
    34381 aacaccattc cgatgaacac gccagaaacc acaataatca gcatcgacag gacgccgaca
    34441 ttatagagct ggcgcaccag cagcggcgca tgtttgcgaa attccggttt gccgaccagc
    34501 gcattgaata acattaaccc ggcccgcccg aacgttctca gggttttaat ccctttatgt
    34561 ccgagcgacg ccagcgcatt taacagcatg agtggcttaa ctccctggta aaagatcagc
    34621 gtgataatcg ccggcaggat agcggaacgg aacaggcccg tcagctatcc cgtccagaaa
    34681 ctgacgtacg cgcggatcag gattcgcctg caacgcctgg gcactgccat gagcgacaat
    34741 ttttttgtcc gccaggatcc aggcgtgatc cgcaatactt aacacttccg gcacatcgtg
    34801 agaaaccacc acacaagtca cgcccagcgc gctgttcagc tcagaaatca gcttcaccag
    34861 tacgcccatg gtgatgggat cttgcccaac aaaaggttca tcaaacatga tgagatccgg
    34921 ctccagcgca atcgcacgcg ccagcgctgc acgccgcgcc atcccaccgg aaagttcaga
    34981 aggcattagt ttagccgctc cacgcagccc cacggcctcc agcttcatca tcaccgtact
    35041 atgcaacaat ggcgcgggaa gttgggtatg ttcgcgcagt ggataggcga cgttgtcaaa
    35101 tacgttcata tcagtgaaca acgccccgga ctgaaataac atgctcatcc gtttgcgcac
    35161 tgtatacagg cgcgaacgag acatcgccgg aatattctca ccatcgaaaa ggatctcacc
    35221 atgatctggt gcgatttgcc cgccaatcag acggagtagc gtcgttttac cgatgcccga
    35281 tggccccatg atcgccgtga tcttccctcg cggcacggtc agggaaatat tatcgaagat
    35341 gcagcgattg ccacgcgtaa aactgacatc gcgcatatcg actaaattcg ccacagactg
    35401 ctccataatt cacccttcgt cttgcgttga ttttctaagc atggcgctca atttaacctt
    35461 gaacccaaca tatttacaga atattacccg ccgtggttag cgaaagctgg catttgtttt
    35521 actttttagc cgcataaagt caaaattaag aatccgttac ggctttctga aa
//
LOCUS       DAZGAN020000042        35118 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGAN020000042 DAZGAN020000000
VERSION     DAZGAN020000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.042"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..443
                     /locus_tag="ACHKYO_RS11355"
                     /old_locus_tag="pgaptmp_002271"
     CDS             <1..443
                     /locus_tag="ACHKYO_RS11355"
                     /old_locus_tag="pgaptmp_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4429114.1"
                     /translation="FGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTKREEYLLMSGD
                     KFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEEVVYVITETVRKTAMQIRWRGMYQR
                     LLKVDPLKCILCGSQMRFTGLKRGYRLAELLLMHERLARQQVCG"
     gene            complement(630..1124)
                     /locus_tag="ACHKYO_RS11360"
                     /old_locus_tag="pgaptmp_002272"
                     /pseudo
     CDS             complement(630..1124)
                     /locus_tag="ACHKYO_RS11360"
                     /old_locus_tag="pgaptmp_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
     gene            complement(1227..1817)
                     /locus_tag="ACHKYO_RS11365"
                     /old_locus_tag="pgaptmp_002273"
     CDS             complement(1227..1817)
                     /locus_tag="ACHKYO_RS11365"
                     /old_locus_tag="pgaptmp_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HGU4429115.1"
                     /translation="MMKNPLAYFHIATFLLSGKSRNLSEYWKAEVIMRDKFVLHRLIR
                     NGLQRQRGFLMWWRLANEMFISGNKKQRKCAIKIKNALMERYGCDIGLGARIGKGLML
                     PHHAGIVIHGNVDIGENVIIRQNTTIGEKVSDSRENYIVIGDNVDIGAHTCIIGLNVK
                     IGSNVKIGAMSFIMEDVPDNCTYVTRKESRVIIREQ"
     gene            complement(1921..2208)
                     /locus_tag="ACHKYO_RS11370"
                     /old_locus_tag="pgaptmp_002274"
     CDS             complement(1921..2208)
                     /locus_tag="ACHKYO_RS11370"
                     /old_locus_tag="pgaptmp_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4429116.1"
                     /translation="MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
     gene            complement(2205..2456)
                     /locus_tag="ACHKYO_RS11375"
                     /old_locus_tag="pgaptmp_002275"
     CDS             complement(2205..2456)
                     /locus_tag="ACHKYO_RS11375"
                     /old_locus_tag="pgaptmp_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGU4429117.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            complement(2529..2720)
                     /locus_tag="ACHKYO_RS11380"
                     /old_locus_tag="pgaptmp_002276"
     CDS             complement(2529..2720)
                     /locus_tag="ACHKYO_RS11380"
                     /old_locus_tag="pgaptmp_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429118.1"
                     /translation="MAESGYGLAGLRMGKVKSINQYRLTPGLGGFTPVPYATTECRLP
                     CRSRGIRTIKPALNVFAKV"
     gene            complement(3415..4287)
                     /gene="repA"
                     /locus_tag="ACHKYO_RS11385"
                     /old_locus_tag="pgaptmp_002277"
     CDS             complement(3415..4287)
                     /gene="repA"
                     /locus_tag="ACHKYO_RS11385"
                     /old_locus_tag="pgaptmp_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HGU4429119.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     DSLDISEEAVASARRSRVEWENKLRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            5125..5631
                     /locus_tag="ACHKYO_RS11390"
                     /old_locus_tag="pgaptmp_002278"
     CDS             5125..5631
                     /locus_tag="ACHKYO_RS11390"
                     /old_locus_tag="pgaptmp_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005082529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HGU4429120.1"
                     /translation="MNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILK
                     KVRRTDKVCGYRRHIHAVFPGYFFLKADPESHSFTHLRRHSAFLDFVKMAGEIKTVRE
                     DIVQSLMKVYPDPALNPAAREELDAASPLWLTKARYQYLLRLDAQPLPESRIALLLHL
                     VSDDGALT"
     gene            5772..6437
                     /locus_tag="ACHKYO_RS11395"
                     /old_locus_tag="pgaptmp_002279"
     CDS             5772..6437
                     /locus_tag="ACHKYO_RS11395"
                     /old_locus_tag="pgaptmp_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HGU4429121.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLES
                     ARQMSAFRGMAEEFTTLRKLLATNTDIPVANTLKSLLESGNNAALYDLYRFRSALAAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            6704..7324
                     /locus_tag="ACHKYO_RS11400"
                     /old_locus_tag="pgaptmp_002280"
     CDS             6704..7324
                     /locus_tag="ACHKYO_RS11400"
                     /old_locus_tag="pgaptmp_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGU4429122.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            7351..9045
                     /locus_tag="ACHKYO_RS11405"
                     /old_locus_tag="pgaptmp_002281"
     CDS             7351..9045
                     /locus_tag="ACHKYO_RS11405"
                     /old_locus_tag="pgaptmp_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HGU4429123.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTAREHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTTPDNSEPQQV"
     gene            9126..9368
                     /locus_tag="ACHKYO_RS11410"
                     /old_locus_tag="pgaptmp_002282"
     CDS             9126..9368
                     /locus_tag="ACHKYO_RS11410"
                     /old_locus_tag="pgaptmp_002282"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HGU4429124.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            9562..10449
                     /locus_tag="ACHKYO_RS11415"
                     /old_locus_tag="pgaptmp_002283"
     CDS             9562..10449
                     /locus_tag="ACHKYO_RS11415"
                     /old_locus_tag="pgaptmp_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429125.1"
                     /translation="MSTSSFIFAILPGILMICIITSLFWVVIYARGKWTFSSGITVFR
                     YLQQYRWFHFGNISLLSLSVILTTRVYDTEYPLQVYICIYIPPVMILTSLLTILTIKE
                     ATRTYKTYKSNRLTPVSVIAYAGLGYMFCFTTGIYELSSTRLNPVISDMLMSFLSRLS
                     IMLPVTLAEIAVIYLLLLALHFPDGVSARGFSVTRYLRHNDWFYYINTGNAILALILS
                     TWIYHSSISLPAALFTGALSIFFNTTIPASLSVIEHTPSDTDSKIWDTRKTSPATVLY
                     CSGTGYVIGFIVNPSAAIS"
     gene            complement(10442..10867)
                     /locus_tag="ACHKYO_RS11420"
                     /old_locus_tag="pgaptmp_002284"
     CDS             complement(10442..10867)
                     /locus_tag="ACHKYO_RS11420"
                     /old_locus_tag="pgaptmp_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429126.1"
                     /translation="MKTKTFRFRKYKKKNVLLQMIVSRKQVILLRNIMTDSLLFCSVG
                     FVFMNMCDSKYTLISGEGGLRADCADYLFFAVYAIALLMIILWMTASVIYSIEEYTSQ
                     EKYGKSKVYSYFWFFIWFFVFNCAVYSSLKAINIKDIIS"
     gene            10984..12051
                     /locus_tag="ACHKYO_RS11425"
                     /old_locus_tag="pgaptmp_002285"
     CDS             10984..12051
                     /locus_tag="ACHKYO_RS11425"
                     /old_locus_tag="pgaptmp_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HGU4429127.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSNALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREARQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNTLLTGQQLYGHMDWNRKILTVTTTAAPPVDLTAPQGSQKTADTPRNPFRSTTGTA
                     ADQAQVPPAVSASRAQSTTGTSGTSAPRTTTPAPVVTKPVAPPQKVTPPRQWKMEKGT
                     TLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFHQALEQLFALYTPVEK
                     PLYARTSTAQCLLRVTDRPQE"
     gene            12056..12493
                     /gene="pilM"
                     /locus_tag="ACHKYO_RS11430"
                     /old_locus_tag="pgaptmp_002286"
     CDS             12056..12493
                     /gene="pilM"
                     /locus_tag="ACHKYO_RS11430"
                     /old_locus_tag="pgaptmp_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HGU4429128.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQHPERRTAGGRLTSAQLGIPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQSNDSALLGRVENGRLLDTAGRALSVTLPSVIPDHVILWMN"
     gene            12524..14143
                     /locus_tag="ACHKYO_RS11435"
                     /old_locus_tag="pgaptmp_002287"
     CDS             12524..14143
                     /locus_tag="ACHKYO_RS11435"
                     /old_locus_tag="pgaptmp_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HGU4429129.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLAYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPPSRLPGNTAAL
                     SELNASGLTTLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVAYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGDVSVVTQNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            14164..15459
                     /gene="pilO2"
                     /locus_tag="ACHKYO_RS11440"
                     /old_locus_tag="pgaptmp_002288"
     CDS             14164..15459
                     /gene="pilO2"
                     /locus_tag="ACHKYO_RS11440"
                     /old_locus_tag="pgaptmp_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HGU4429130.1"
                     /translation="MTDISPTGRQVFPLTAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLSNEDADTHTMVATVSRRILPGKPRQCFSLALLILPLLEHGGYAITEL
                     TLPGETPRYSFVSVVDGVLVSDLVGSGEEVREARDTFLSINTEPTQGWIHYEPAAFSA
                     GDQNQHLPLASLTGSGKHPAAARLNPVSRGPQFITVSLIIALLGTAWYGWQYYERWQT
                     EKAAQAAVARQATEERITPPWPGLPETDAFIRGCADIWTSLPLSAAGWRYSLAECSTD
                     GGSGNLRASYTNTAGATVTDFIRRISDMTDSRPFIVMPEGKTGGIGLPVTFRLSENAV
                     TVDALPETAVLQERLTSLAQSMQLKLDWQEVNNSVTDENGQIIQPPWKEYDLQIQTTL
                     PAHHLAERLKEPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            15449..15901
                     /gene="pilP"
                     /locus_tag="ACHKYO_RS11445"
                     /old_locus_tag="pgaptmp_002289"
     CDS             15449..15901
                     /gene="pilP"
                     /locus_tag="ACHKYO_RS11445"
                     /old_locus_tag="pgaptmp_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HGU4429131.1"
                     /translation="MPDKAKTLLFSVLLLPATAMATTGNAEQQNTQQIRIEQIQEETA
                     LYEAMLAREKIRHELRTLEQGVPAEAGRQQESISAVTTNVQALPSPRLTAIFGVHPQL
                     TARIELPGGSISDVKAGQNIPGTTYHVEKITPDSVVLEQSGTRKILRP"
     gene            15986..17494
                     /locus_tag="ACHKYO_RS11450"
                     /old_locus_tag="pgaptmp_002290"
     CDS             15986..17494
                     /locus_tag="ACHKYO_RS11450"
                     /old_locus_tag="pgaptmp_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HGU4429132.1"
                     /translation="MLDPADLPPDDILDYVAIDTDNAGQLRIRVVEGKKHLRAVQEYL
                     TRLRARYQGRVGDFEFTTLDVIARLRQDATTTGDENAINPIQQKMLGYIRHSASLGAS
                     DLHMTPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQQIPTFSILGYHPDQE
                     CTLRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTTRGWVDSIKSALRLDPDSILNGEIRDHDSAITAIKAAMTG
                     HLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRHPWNDV
                     ASSRTKDERHLVETFCNADAVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQLYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGLVDPLAAHYISPIDEDSYTLLR"
     gene            17496..18596
                     /locus_tag="ACHKYO_RS11455"
                     /old_locus_tag="pgaptmp_002291"
     CDS             17496..18596
                     /locus_tag="ACHKYO_RS11455"
                     /old_locus_tag="pgaptmp_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HGU4429133.1"
                     /translation="MKFLEKLNSTELDLSALNSWTARKTFGLKRAEFYESLGGMIRDG
                     TPSLRALEFICNVETDFGTRKGTSGMYFLARECIASLKASGMLSGALPTWVPPEEAAL
                     IRNGEERGNIAEALFQVAKTVKARREMVSSLITLCLYPLLLLSLCVENMYNAHTRLIP
                     IVSAFAPEEKWSFQMKVLAAMSELVIGYGLWISGAIITLVIIIRFSLSRYTGPGRRLL
                     DKLPPWSLYQTFHGVNYLFSISSMLQINIPLSQALLRIEKSAEHNKWLKERIQAIRKN
                     VLSGQNLATAMRNSDYDFPSKICINKLLLNSEREDSVSAMNTYAGQWLEDAKNSVRKS
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            18670..19206
                     /locus_tag="ACHKYO_RS11460"
                     /old_locus_tag="pgaptmp_002292"
     CDS             18670..19206
                     /locus_tag="ACHKYO_RS11460"
                     /old_locus_tag="pgaptmp_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HGU4429134.1"
                     /translation="MNFRARLSSYFSLKKDKEDDKGLTLLEIAGAMIIIAIVVALAMS
                     QMSSTMASSDNQRELKNLQTIATKMKQYKFQGRYTGTDYIKTLTESGALPADMIAGGN
                     KAKNAWGGDVTIAATADKYGYIITSNNVPKTNCVELINSLRSSSIFTKIMNTAPNTVD
                     PVTVCSSDKNNIKLETNS"
     gene            19260..19733
                     /locus_tag="ACHKYO_RS11465"
                     /old_locus_tag="pgaptmp_002293"
     CDS             19260..19733
                     /locus_tag="ACHKYO_RS11465"
                     /old_locus_tag="pgaptmp_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HGU4429135.1"
                     /translation="MKWFYLSSLFLFCLPATAAVNSNTLDSCFIQAGKRYSIDPGLLK
                     VIARKESSLNPRAINHNRNASGKIISTDYGLMQINSTHIPGLKRMGIIRSENELLSNV
                     CLNIQTGAWILARHFQQCGVNWECLGSYNAGFNKNNTHRRMKYARLIYAAYMQGR"
     gene            19752..20387
                     /locus_tag="ACHKYO_RS11470"
                     /old_locus_tag="pgaptmp_002294"
     CDS             19752..20387
                     /locus_tag="ACHKYO_RS11470"
                     /old_locus_tag="pgaptmp_002294"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HGU4429136.1"
                     /translation="MFLIILLVISATLTIQLLAIRVAGHYLMQVHTLRLPRHLSLLCV
                     VLALTATITHIIRGTDITGLCFLLLMAGCMTLMTLTDATSCRLPRPFTLTFLMLGVAF
                     RLWQGDAAIALACALFWFLLLSLLRRYTSYRKGVETLGLGDVFLIAGVAMWSSANSTP
                     WIIGIAATGTMVCMSLFPRSNTGAAIPFGPFICASLYIFTFFPGAIFHTGG"
     gene            20392..21624
                     /gene="pilV"
                     /locus_tag="ACHKYO_RS11475"
                     /old_locus_tag="pgaptmp_002295"
     CDS             20392..21624
                     /gene="pilV"
                     /locus_tag="ACHKYO_RS11475"
                     /old_locus_tag="pgaptmp_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HGU4429137.1"
                     /translation="MQKDDDKGFVLFEILAGLIVIGIATPMIYSEIENWLNEQLYQSA
                     AYHADAYNTAARNYIADNNARLHSGSLPANFTADDLIRQGYLKQGFNHSPFGQSYITG
                     IRRNQTTGRLEALTCSTGGQNIKEDGLRSVAGQLPGLGGYIGKNGTATGAFGAWTDKP
                     GDYGLTCSAGHIAVVMTGDDLQESDRLYRFQVPGRPELNQMHTAINMGGNDINNAGNL
                     NGQKATVKGDITSEDGWLITRNNKGWMNTTHGGGFTMTDSQWIRAVNNKGITTDGEIK
                     GGKVSGGTIRSDGRLSTGEYLQLEKTATAGTSCSPDGLVGRDSKGAILSCQSGVWKNN
                     ISDALVFDYSACYDAEEDSRKPVYANWGSGSNGFAVCDGKAGYGAAGFRAYRGKPGYS
                     GSMVWVKIRCCKLKVNTP"
     gene            complement(21630..22031)
                     /locus_tag="ACHKYO_RS11480"
                     /old_locus_tag="pgaptmp_002296"
                     /pseudo
     CDS             complement(21630..22031)
                     /locus_tag="ACHKYO_RS11480"
                     /old_locus_tag="pgaptmp_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            22083..23249
                     /locus_tag="ACHKYO_RS11485"
                     /old_locus_tag="pgaptmp_002297"
     CDS             22083..23249
                     /locus_tag="ACHKYO_RS11485"
                     /old_locus_tag="pgaptmp_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGU4429138.1"
                     /translation="MFQKKTRKMSLSRALDKYLKTVSVHKKGHLQEFYRVNVIKRHPM
                     ADRYMDEITTIDIAGYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGTC
                     RMNPVELVRKPKISNGRDRRLTSGEERRLSRYFRDKNPQLYVIFHLALETAMRQGEIL
                     SLRWEHLDLQHGVAHLPETKNGSPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFKS
                     AWRTALLDLKIENLHFHDLRHEAISRLFELGTLNVMEIAAISGHRSLNMLKRYTHLRA
                     YHLVSKLDARRKQTSKISPYFVPYPATVGCRNGLFVVTLHDFDLETRAETRELAISHA
                     SVLLLRALAQAAQRGERVPTPGELPANIDERVMICPLTNSQFMTKANSIFISSS"
     gene            23291..23581
                     /locus_tag="ACHKYO_RS11490"
                     /old_locus_tag="pgaptmp_002298"
     CDS             23291..23581
                     /locus_tag="ACHKYO_RS11490"
                     /old_locus_tag="pgaptmp_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429139.1"
                     /translation="MKHLSSRAATADNNRIVTIEDVYTIAFSQPIHTESALGQRWMLI
                     HTGEYESASDAKRFLDTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDAK"
     gene            complement(24011..24346)
                     /locus_tag="ACHKYO_RS11495"
                     /old_locus_tag="pgaptmp_002299"
     CDS             complement(24011..24346)
                     /locus_tag="ACHKYO_RS11495"
                     /old_locus_tag="pgaptmp_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429140.1"
                     /translation="MKIKQHKEKLEIIRNIIRETLLGNAAIVVLVSMIFVMLEKPWSV
                     MSVLCIFVYCISLMIVSLWWFFIVFIYISELTESVNIMRVLVAIWVLSSESFIIYNII
                     GVFHKVFMY"
     gene            24608..25429
                     /locus_tag="ACHKYO_RS11500"
                     /old_locus_tag="pgaptmp_002300"
     CDS             24608..25429
                     /locus_tag="ACHKYO_RS11500"
                     /old_locus_tag="pgaptmp_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HGU4429141.1"
                     /translation="MKPDSTGIATRMMLSLDRERICECLLSNRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEEALSEMFLPADNIVRRLLCRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGDILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PVTQEAFCRIISSGENVDIILWHGFPFAGTHSGEYCGVLLRQKKEINNAIIYPVLSLY
                     PVRMDMEKEGWFQMVRKISPDIPVRAVTLTPAQAEYLITGKILPTVLFRVSLSPW"
     gene            25533..26735
                     /locus_tag="ACHKYO_RS11505"
                     /old_locus_tag="pgaptmp_002301"
     CDS             25533..26735
                     /locus_tag="ACHKYO_RS11505"
                     /old_locus_tag="pgaptmp_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HGU4429142.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPEDDFSLLIPSVGGQLSDPGNISDNADKISDDWKAFDRAID
                     SQSNVSQTAARLKTRLQDFRHTHADAQVGTSAVATLPGDTLAAALMLKSYGTVSVDGK
                     VSDADLNYLESIANSNGQDVDKDRLTSQAFARAALVTDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVKNYDSSAFKGDRYHNTRNGINADIGASMDLDDNWTLGLVAQN
                     LLPRSIETKEVNGVTETFRIRPQVTAGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AIGAEFNAWKWAQLRAGYRQNLASNDGSAFTAGLGISPFDVVHIDVAGIAGTDNNYGA
                     VAQLQFTF"
     gene            26875..27045
                     /locus_tag="ACHKYO_RS11510"
                     /old_locus_tag="pgaptmp_002302"
     CDS             26875..27045
                     /locus_tag="ACHKYO_RS11510"
                     /old_locus_tag="pgaptmp_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429143.1"
                     /translation="MFNKNKTHIENKAFMVEPFRDPTANECNVVIANDICIRVALSPS
                     EVLQPFSKNTNQ"
     gene            27094..27546
                     /locus_tag="ACHKYO_RS11515"
                     /old_locus_tag="pgaptmp_002303"
     CDS             27094..27546
                     /locus_tag="ACHKYO_RS11515"
                     /old_locus_tag="pgaptmp_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081199.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HGU4429144.1"
                     /translation="MNRMIPAGCLAVLCLNGCHLPPDNTAPTRKYGAASDVAVARHAQ
                     YQLWGQGIYSAALPERQSGGSLKAHSDRVSFDWEGDALELLNELARLRGQQFSYSGVR
                     LPLPVSLHVRDMTFADVLRLTEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            27543..28379
                     /locus_tag="ACHKYO_RS11520"
                     /old_locus_tag="pgaptmp_002304"
     CDS             27543..28379
                     /locus_tag="ACHKYO_RS11520"
                     /old_locus_tag="pgaptmp_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HGU4429145.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            28363..29511
                     /gene="traJ"
                     /locus_tag="ACHKYO_RS11525"
                     /old_locus_tag="pgaptmp_002305"
     CDS             28363..29511
                     /gene="traJ"
                     /locus_tag="ACHKYO_RS11525"
                     /old_locus_tag="pgaptmp_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HGU4429146.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSSIMMALSDELFGREVLPRVLSGHPVDRTVQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVMTDELRNTLSGQS
                     HATWGHYIDEIIRQEKRRIRDQVLTMYQNDRIEKSEAALFIPAGEFPR"
     gene            29508..29798
                     /locus_tag="ACHKYO_RS11530"
                     /old_locus_tag="pgaptmp_002306"
     CDS             29508..29798
                     /locus_tag="ACHKYO_RS11530"
                     /old_locus_tag="pgaptmp_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGU4429147.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWTVRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            29862..33893
                     /locus_tag="ACHKYO_RS11535"
                     /old_locus_tag="pgaptmp_002307"
     CDS             29862..33893
                     /locus_tag="ACHKYO_RS11535"
                     /old_locus_tag="pgaptmp_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HGU4429148.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAE
                     AGTDLSLLKRWLPRPQEMSMDAGDPVTEFRQVLENAGLVLEKDPEMDGEIHRVPTRDD
                     RRGSQSGAYKAYLDGRPSGWYRDYRSADNSPTTWTFSRAENIDPLARLHLKASAQQNR
                     DEKERKLQQQYNKQAGYARSYLNGLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKGSEKSGNWFAFGTPENGRPLLFAEGYATAASLHE
                     ATGHPVLMTIDADNMINVAKNARKIWTDSPFVFCADNDHQRKINKGVSSAKKAAASTH
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDAFQHVMNAQLKHIGIPTPNSDIADHREA
                     VVIGNLIFTPVKDEKPQMSPENKQSTAPETVLATPDTPYDIHTEETENQAYEDDYAAY
                     ASLQSVTTETLSPDKPLENIASAADTGTENTSAATAGDQEDLNTDATGTDTAHNTNTA
                     ETGTLPEPAEIQPVQKSSPEPTAEDKPQESVIPAADTGEKNASATTADNQSVQAEELS
                     PGDDTPETSVSDATGTQHTTTTQTEEQLQVSMASADDWSAFAEDLNADATVMTTAHHT
                     DTTETASPSEPAGTQPVQQATPEATAENKPQEEDVLSATDTSEKSASAVPDNNRDAPP
                     EELNADATVAATVHNTGTAETASPSEPAETQPVRQATAEPTAEDKPQEERLVSATETS
                     TANEAARPETPSLFSRLRRFLTGGKSRTSEPSVIQEKKPTEAETATETAAPPSPPETS
                     PEDTSSAEPDTIIFAPRRPDSPQRHNLDEILKNLEHKEFPDRTALYSVDGEPAFLDRL
                     YCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIVEYDIKVRMKL
                     PAQRAALEKLRKEKEMVHDAIVTHRPTPELNRQSPEETTMAQPEPPMSPKAEAEQTAG
                     CTAETPHPEAPVTAKAEAPRADRPEQPAAPSSAPEKAPVSAQPQQPDTPEEEPPGKLS
                     PGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGKGLEELVKNHQKGDPVTL
                     TLQARERSPSSPGKPEWVRNIWTMKPVCNGISVSHDNPHEGQYIREYPAATFRQMMTL
                     LQQGWPQLMQDVRLPDKVPDTLYLGEDRQPAAAPRDPALRVPLNGTPPATLTPLIFSV
                     DKQSQRLDLLLLQSADEYMQGVVRLNGTLYPALATPTADSRQLVINAMTDRGLQFAGY
                     GEAINRDADGPKRPAPQLMQFHLKQQDSPLFAAIHKPEEQPDKLFRSLGFEQTWKEWS
                     DSQKAEDRQEKTLQQAQSHSPGL"
     gene            33910..34260
                     /locus_tag="ACHKYO_RS11540"
                     /old_locus_tag="pgaptmp_002308"
     CDS             33910..34260
                     /locus_tag="ACHKYO_RS11540"
                     /old_locus_tag="pgaptmp_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGU4429149.1"
                     /translation="MNMKKTLLVILTSISLASPAAVAATCRAGAAAEAGSKAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDVSITLPQFPSLDKILRDLENQVCDAVVDRVNE
                     HLPGNIDPWKDYNL"
     gene            34272..34967
                     /locus_tag="ACHKYO_RS11545"
                     /old_locus_tag="pgaptmp_002309"
     CDS             34272..34967
                     /locus_tag="ACHKYO_RS11545"
                     /old_locus_tag="pgaptmp_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HGU4429150.1"
                     /translation="MSEAHPTSPVSPETDNSGDIYKQTLSTIAQSEQRAALVPGLVRA
                     LLCSSAALVISLTGNIIQYWHNSNVTREYFATDNGRLVRLAPTSQPAWSQSDVMTFGS
                     MTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTATIG
                     AGTLVNQGQMENGIWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAASGT"
     gene            34978..>35118
                     /locus_tag="ACHKYO_RS11550"
                     /old_locus_tag="pgaptmp_002310"
     CDS             34978..>35118
                     /locus_tag="ACHKYO_RS11550"
                     /old_locus_tag="pgaptmp_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HGU4429151.1"
                     /translation="MQIHHLLGALLIVCGTVTAADNPGWQNARTPQAGGKTGQAPGNA
                     PAG"
BASE COUNT         9022 a         9529 c         8515 g         8052 t
ORIGIN      
        1 acttcggctc ttacctgaaa aagccaccgg tgccgatgag tcggctggaa cattatgccg
       61 gtcaggatga aatcggtctg cgttacaaca gtcaccgtac aaaacgggaa gaatacctgt
      121 tgatgagtgg agataagttc atggaaaggt tctcctggca tgtagcagat aaggggttcc
      181 gtatggtgag gtactacggt ttcctgagtc cggtgaagcg ccgcttactg gaagaagttg
      241 tgtacgtcat aacggagacg gtgagaaaaa cggcgatgca aatcaggtgg agagggatgt
      301 atcagaggtt actgaaggtt gacccgctga agtgcattct gtgcggaagt cagatgcgtt
      361 ttacggggct gaagcggggt taccgactgg cagagctgct cctgatgcat gagcgactgg
      421 cacgacagca ggtgtgcggc tgagagcctc agaggggaag ttgcgtccat tttaccggaa
      481 acggagcaaa aaaccgccat tcataccctg tatcaatcag tgtcatcctg tttaatagtc
      541 gtttccgctc atatggtgca caaggggtgt tgaagaaaca tccgctttgt ggtgcttttt
      601 tagtcttttg gggatttaaa ttcctatcga tgaactttgc catccagacc ataacgcgga
      661 gtacctgcga ctgatatacg ctgcagatat ttgtaaccgc cagtaatcat ccttacagca
      721 caattccatt cttttacagt tagtccacac tcaggactgg ccgaaataaa agccatgaca
      781 tcctttgaga tgcctatttt aaatgcatga tatcccccct cttcagcccg taactgaggg
      841 aatgtttttt taacacagtc cagactgtgt cggataaact catgattcca tctattgttc
      901 ttcttttttt tcttcttctt acgtcctgtt tttttctgtt gttccgggga accggccact
      961 gtggctggta tttcaggagg taatgagtcg gttttaaatt ccattactgg cgctggtttt
     1021 atatcatcag gaactgcctg tacggcatca tcctgatgag aaagaattcg cctttttttc
     1081 ttaataataa caacaggtga cggtttcttt gtaacactgc ccatatacac acctctgcgt
     1141 atcgttaata tataatgcac gacatacaga tagtatatat ctgagtcggc aaagtcacca
     1201 acagaagaat gataacatgt atttcattac tgttcgcgaa ttatcactct ggattccttc
     1261 ctggtgacat aagtacagtt atcaggtaca tcttccataa taaaagacat tgccccgatt
     1321 ttaacattac ttccgatttt cacatttaac ccaataatac atgtatgggc accaatatcg
     1381 acattatcgc caataacaat atagttttcc ctactgtcag aaaccttttc accaatggtt
     1441 gtattctgcc gtataatgac attctcaccg atatcaacat taccatgaat gacgattcct
     1501 gcatgatgag gtaacatgag gcctttacca atccgcgcgc ccaggccgat atcacatcca
     1561 taccgttcca taagtgcatt ttttatcttt atcgcacatt tcctttgttt tttgtttcca
     1621 gagataaaca tctcattggc caggcgccac cacatcagaa agcctcgttg tctctgcaac
     1681 ccgtttctta tcaggcggtg taagacaaac ttatcccgca tgatgacttc ggctttccag
     1741 tattctgaca gattacgact tttacctgac aataaaaagg tggcaatatg aaaataagct
     1801 aatggatttt tcatcataaa aacgacctta aagaataaag aaaatattct gtattgtgga
     1861 ataaaataac ataactatca gaaatacttt taaagtaata tcagtcagct ttaaagataa
     1921 ctatctcatt ctctcactgg cgagattata aacgttacta cgttcacgtt tacctaccgc
     1981 gacaaccgca ataattagca tgtcatcaat aacctgatag accagacgaa accccgatgc
     2041 gcgaagtttg attttgtagc agtcttttaa tccacgcagt tttgcggaag gaatatgagg
     2101 attttcgctg catttcttta gttttttcgc aaactgctgt tgaatggatt tatccagttt
     2161 cagccattct ttgagcgcat catccctgaa ttttactgta taagtcataa ataactgtcc
     2221 aggtccacat catgcagtgg ctgattgctg cgttcagtaa ccagtttcac cagttcctgg
     2281 tcatccagcg catcaagcat cctttcatag agttccgcag gcacacagta gaatgccggc
     2341 tgattccggt taagaatggc gacaggataa ccatctccgg cactgactgt agccatagga
     2401 ttttttttca gttcactgac acttgcactg gtgtcagaca gaatgatgtt aggcataacg
     2461 tacctttgag actgtttaac aagtcttaaa gatacctctg aaaggaccgg ttaacaagtc
     2521 tgtattactc atacttttgc aaatacattt aatgctggtt ttattgttct gatgccgcgt
     2581 gagcggcatg gaaggcggca ctccgttgta gcatatggca caggagtaaa accgccgagg
     2641 cccggcgtaa gccggtactg attgatagat ttcaccttac ccatcctcag ccctgctaag
     2701 ccatacccgc tttcagccac gacagagctt atgtgcgcgg tcggagtggt cccgacgagg
     2761 gtttacccga agtcggggcg tatctccgcg ttagcgggcc gtcagggccg cttacgagcg
     2821 tgtactgaga acatctagcc agaagactga cagcaatgaa gatgtttagt tacaacattc
     2881 ataattaaaa gcgatgttgt tcccgccctt cgggcggggg cggcctcgct tttcagttat
     2941 gagggatggg cacggtgctg cgtttgtgtg gtgtttttat tcctgatgtc tttttgctgt
     3001 ggtttcagaa cggtgaggtt cggggcccgc gcgttagcgt gggaggtgca tatctgtctg
     3061 tccacaggac aggcattgaa taggttttct ttttaaatgg aagtaattaa gtagtttaaa
     3121 ggaaatacag tactggggtt taaaagaaac attgcaccct gtaacgccta cggctggcac
     3181 tttatggtat gaacggttgt aaccttatgg agaagtccct tgcagttaaa tgtggacaag
     3241 aagaattccc tgtctgtgag acgtgttttg cgtgaaaaac agggggaacc ggatgcacct
     3301 gaaggtggat gacgtggctg ttttttgtgt gtggggctgc ttttttgtgc gcttgcgggc
     3361 ttcaggcgtg cggatacctc cggcgcaggc cggattattc agatgaggtc aaactcaggg
     3421 tgaggcagtg gccagccgtg tgtagttgtt gttccgcgac attatcatgc gttccttcac
     3481 gcgacggtcg atttcgcgct tcacggcctc caggcttccg cggaaacgcc cttccgcgat
     3541 ttcacgtgtc agctgccgtt tcaccagggt gacaatgtcc tgacgggtcc tgcctgcatc
     3601 gcggcgcgca cgggcacgtt tcatgccgtg ggacttaagc tctgcctgat aactgcggaa
     3661 acgctcgcgc acaaaacgcc aggctctcgc tatcagttca tccataccca gcgcatccag
     3721 cccctgcttt ttgcgcagct tgttttccca ttccacgcgg ctgcggcgtg cggaagccac
     3781 tgcctcttct gagatatcaa gtgagtcaaa cagtgccggc gtgaacgtga tgtcggtcgg
     3841 gatgttacag ccaattgtcg ggtcgtattc cgtctggtag gttatcagcc ccagctcaga
     3901 caggaacttc agggcacggg tggcacgggt gatggagagt ttcccggcct cagactccgt
     3961 cgccagtccg cactcaatgg ccagcgtggt gatggagcac tggacgcggt tggccagcgg
     4021 atcgtagtgg aaacacagcc cctgcagcag cgcatcaatc gcccggcggc gcagcaccgg
     4081 cggcatacgc cggcgcaggc cgcgcgaacg ggcatacgcc acatgcgcgg caaaatcaaa
     4141 acgggaggtg aagcccaccg ccttttccat cagtttttcg cagaacttca gcgtcccggc
     4201 atcttcacgg ggtgtgaaga ccggattcgg gttctttacc tgacggtatg tgctttcagt
     4261 aaatgaggtc tggttcaggg ccgccacaag ctgtcgctta tggcggaaac agaatgccgt
     4321 gcgttaatgc actgcgaagg gttgaatctc tgacacggtt tcatatatac ttaccccgta
     4381 ttctgtgagg cccccataat cttctctacg ttccgccaag ttcgataaga agattatggg
     4441 ggttttcctt tatggtcttc tgaaactttt ttcagccttc cggccccggc caaccgccgt
     4501 cgtttctgcc atacatcctg aatgccttat ggcgcatcct gccgttttct gactttccgg
     4561 atcgcgagga tcccaaaagc cactgcataa cgatccgcaa tatacctcgt ttccaccgct
     4621 ttgaaaagcc gacatgattt ctcagggtta cagttgccct ctttaactcc ccgaaaaagc
     4681 gtcgtttttt tgaactgtgt caaaaaaatg aacaattccg caaacgatct tttatgctgt
     4741 gtttttcatg ttttttggat gaatcgatct tgtaattacg atctacgatc caatcatcca
     4801 aacacctcac acaaaacagc ataatatgct gttttatcaa atataacgat ctattcaact
     4861 gcttatcttc gctcttgcca cagatgcatc cttatgtaat atatttgcca gacattaatc
     4921 ctgtttgcag gagttgtcgg ttttcgcccg cctcggaacc gtccacaagg cttacaagag
     4981 tgcgtaaatc atgtgcctgt cagaactgca ggccgggctg aacgagggtc agccgctgtt
     5041 tccgacacat gctttatgta ttcgccgttc acaacaacat cgaagccccc tgcggggatc
     5101 gattttcgcg gagcgttccg gattttgaat atcagtgaat tacatcaacg caactggtat
     5161 ctggcacagt acatccctgc cggcaaaaac cgcgaacatc tcttttcctg gctttccgaa
     5221 caacacgtcc ttccctggac acccctgata ctgaaaaagg tccgccgcac ggataaagtc
     5281 tgtggttacc gtcgacatat acacgccgtg tttcccggtt actttttcct gaaagcagac
     5341 cccgaaagcc acagcttcac tcaccttcgc cgtcacagtg cctttctgga cttcgtgaaa
     5401 atggccggtg aaatcaaaac cgtcagggag gatatcgttc agtctttgat gaaagtgtac
     5461 ccggatcctg cgctcaatcc ggcagccagg gaggagctgg atgctgcctc ccccctctgg
     5521 ctgacaaaag cgcgttacca gtacctcctc cggcttgatg cacagcctct gccggagtcg
     5581 aggatcgccc tgctcctgca cctggtatct gatgacggag cgttgacatg aaaattgctg
     5641 tcttaatttg acgacttttc ccggccttta tggccgggat ttttttccct tctgtttcca
     5701 caatgcccgg caccggtgac accttacggt gtacggagag cggctgtcgg gcattttttc
     5761 aggacgcctt tatgaccatt ctatcccagg aaaaccctgt cattctgtta ctggagaata
     5821 tgctcgaagc actctccacc gccccggaca acataaacaa cgaacggcgt cgccggcgtt
     5881 acctgctgaa ctggcttgaa tctgcgcgtc agatgagcgc attcagagga atggcagaag
     5941 agtttacgac tctgcgaaaa ctgctggcca ccaatacgga cataccggta gcaaatacgc
     6001 tgaaatcact gctggaaagt ggcaataacg cagcattata cgacctgtac cgcttccgct
     6061 cggctcttgc cgcatgcaga agtctgggat ggcagattgg cacgtgcgca tggaacgacc
     6121 agctgctgag tgagacactt tcccgtgcgc aaacaggtaa acgtcatatc ctgcagctga
     6181 acagtaccag agacaccttt tccaccacgg gcagaatgct cagaccggtg gcattttcat
     6241 taattcatcc ggcatccctc gaatcttcag aagtggaaga gatattccgg gatgaaggtt
     6301 ttatactcgc gcacggacgc gaatgcagcc tgaacggcag cggcagaaac atgctgtcac
     6361 gcattcttca tgtggggcat tcaggtttac cgaaagccga atggggcata gatcacagca
     6421 atcacctgtt acactgaaat accgggacag ccacaacggc agccactctc cgctgacttt
     6481 ttttcgcaaa tcgcggtttg ctgatgtgga tgtcccattt tttattatcc gtcctctccg
     6541 gttgtcataa agcgcattcc ggcgtgaggc ttctgttgtt atcatcccct cctgcagaca
     6601 tccccttgtc ttctgatgac tgacgatatg cttcttctga caattaaaga agcggaagct
     6661 tcacgccata atgttgtttg actgacctgc cacaggagga aaaatgacac tgacgttact
     6721 taccgcgtat aacgcaccct atctggcagc cctgactttt gttctgctta ctggtatcgc
     6781 tgaacttatc gccctgctct gcggacactc cctctcttca gctatggata cacctgattt
     6841 accggagggg ctgacaggag aggcgctgga ctggctgaac atcggacgga tccccctgct
     6901 gatcgtcctc tgtatgctgg caggtttttt tggtatatcc ggcattctcc tgcagggtct
     6961 tattattcat ctgctgcagg ctcctgcccc gaacattctc cttgcaccac tttgcctgct
     7021 gctgacatgt cctctcgtac accgcaccgg cagactgatt gccccctggt taccccgtga
     7081 tgaaacaacg gcgctcaccg aggaggcatt catcgggtgt atggccacca tcaccggtta
     7141 tcacgcagtg gcgggttccc cctgtgaagg tcgcttcaca gaccgctttg ggcaggttca
     7201 ttacctgttg ctggagccgg aggccgggaa agaattcaga cgaggagaca aagtgattat
     7261 tgtctgccgt ctttccggtt cccgctggct gggcgaactg aatccctggc cggatgttct
     7321 ttaatcccga catatacaga ggttctcact atgtttaatc ttccgggatg gttgtttacc
     7381 gttattgcgc tggtcctcac cctgttcgtt atcgggctga tatttgcccg cctgtaccgt
     7441 cgggcatcgg cagagcaggc ctttgtccgc acaggtctgg gaggccaaaa agtggtaatg
     7501 agtggcggtg caattgttat gcccattttt cacgaaacaa ttcccgtcaa catgaacacc
     7561 ctgaagctgg aagtcagtcg tgcagcggct gaaagtctca ttacccgcga ccgcatgcgt
     7621 gttgatgttg ccgttgcctt ttttcttcgg gtaaaaccat cagccgaagg aatatccact
     7681 gcagcccaga cgctgggaca acgcacgctc acacctgaag atctgcgtag tctggtcgag
     7741 gataaatttg ttgatgccct gcgggccacg gcggccagaa tgagcatgca ggatttacag
     7801 gatgcccgcg agaactttgt tcagggtgtg cagaacaccg ttgcggaaga cttatccaaa
     7861 aacggtctgg agcttgaaag tgtgtcgctg acaagtttca accagactgc acgggaacat
     7921 tttaatcctg acaatgcctt tgatgctgaa ggcctgaccc ttctgaccca ggaaacagaa
     7981 cgtcgtcgcc gcgaacgtaa cgaagttgag caggatgttg aagtggccat cagggaaaaa
     8041 aaccgggatg cactctcccg acgtctggag attgaacagc aggaagcttt catgactctg
     8101 gagcaacaac aacgggtaaa aacccgtacg gcagagcaga gtgccagtat tgctgctatt
     8161 gaagctgaac gacgtcgtga agcagaatcc gcgcgaattc tggctgaacg taagatagag
     8221 gaagccgaaa tcgaacgaca gcagatagtg cgtacccgcc aggtcgaagc cgaacgggaa
     8281 gtggccatac gggaaattga gcaacagcag gcaaccgaaa tcgccagcca ggccagagcc
     8341 attgccgtgg cggcaaaatc ggaagagcag tcacaggcag aagcccgggc aagtaaagca
     8401 ctggctgaag ccgtacaggc ccagcaggat gtggaaacca cccgccagac tgcggaggcc
     8461 gaccgtacca aacaggttgc cctgattgct gctgctcagg aagctgaaac gaaagcggtt
     8521 gaactgacgg ttcgcgccca ggctgaaaaa gaagcagccg aactccaggc agcagcaatt
     8581 attgaactgg cggaggccac ccgtcagaaa ggtctggcag aagcggaagc acagcgcgca
     8641 ctgaacgatg ccattaacgt gctttcggat gagcagacca gccttcggtt ccgtcttgcc
     8701 ctgctggatg cattacctgc cgtcatagag aagtccacag aaccaatgaa atccattgac
     8761 ggcattaaaa tcattcaggt ggatggcctg aatggcggtg ttacccgtgg taaaacagca
     8821 acaaccgaca taagtggcgg aaatctcgct gaacaggcgg taaccgccgc tctggcttac
     8881 cgtacacagg ctccgctcat cgactccctg ctgaaagagg tgggtttatc cggtgaagga
     8941 cttacagccc tgacacgtcc actggtcatg cctgccccgg aaaaggttcc ggatgtcagc
     9001 gaaccggtaa ccactccgga taactcagaa ccacaacagg tttaatcccc gtgccggaat
     9061 ttttccggca tctttttcac aaagaatgcc ctcacgggat attctggttt aaaagaggat
     9121 tgaaaatgtc cgcagaaaaa ttcagagttg tcatatacac caatacatgt gaaaagaaag
     9181 tcagttatat tcctgcaaca gaagatataa acatcgctat aatgccattt cagacggcag
     9241 acaatggctt aattattgtg tgtcagaact cccgtataat acgacgcaaa gagcgtatcc
     9301 ccggaagcag ctgtgaatgg aaagatgtga cattagcacc aaaatccaga aaccgcgttc
     9361 agttatagcc tgacagcctg cttttcatcc cccataaaag ggggataaaa actaaatatt
     9421 tcacaaccgc gatatttttc aggaatgcag attattttta tatatgttgc tgacgctatt
     9481 attcacaact acacatgata ttgattacgc acctcacaac cgtaaaaatt taagcatcac
     9541 catgctgatg gagatttaat aatgagcaca tcatcattta tttttgcaat attacccggc
     9601 atactaatga tatgtatcat tacttcttta ttctgggttg ttatttatgc tcgcggcaaa
     9661 tggacatttt cttcaggcat cactgttttc cggtatcttc aacagtatcg ctggtttcat
     9721 ttcggaaata tatccttact gagtctgtcc gttatattaa caacccgggt atacgatact
     9781 gagtatcctt tgcaggttta tatctgcata tatataccac ccgttatgat tctcacatcc
     9841 ctgctgacaa ttttaaccat taaagaagca acgagaacgt ataaaacata caaatcaaac
     9901 agactgacgc cggtttccgt tattgcttat gccggactgg gttatatgtt ctgttttaca
     9961 accggcattt acgaattatc ttcaacacga cttaatcctg tcataagtga catgctgatg
    10021 tcattcctgt cacgtctttc cattatgctg cctgttacac tggctgaaat agcagttatt
    10081 tatctcctgc ttctggcgct gcatttcccg gatggcgtgt ccgccagggg gttctccgtt
    10141 acccggtatc tgcgccataa cgactggttt tattatataa atacaggaaa tgctattctg
    10201 gcactgatac tctccacatg gatatatcat tccagcattt ctcttcccgc tgctttattt
    10261 acaggcgcgt taagtatttt ttttaacacg accattccgg ccagtttatc ggtcattgaa
    10321 cacacgccat ctgatacaga ttcaaagata tgggatacac gaaaaacatc cccggcaaca
    10381 gtcctttatt gctcgggaac aggttacgtc attggtttta tcgtgaatcc ctctgctgcc
    10441 attagctgat tatatctttt atatttattg ccttcagaga agaataaaca gcacagttaa
    10501 aaacaaaaaa ccagataaaa aaccagaagt agctatagac tttactttta ccgtattttt
    10561 cctgactcgt atattcttca atagaataaa taactgaagc tgtcatccat aaaattatca
    10621 ttagcaacgc aattgcatat acggcaaaga ataaataatc ggcacaatca gcacgtaaac
    10681 caccctcacc agaaatcaac gtatattttg aatcacacat attcataaac acaaaaccta
    10741 ccgagcaaaa taataaagag tccgtcataa tattgcgtaa caatatcact tgttttctgg
    10801 aaactatcat ttgcagcagg acgtttttct ttttatattt ccggaaccgg aatgttttag
    10861 ttttcataat caccccgaac atgaatgttc atcatcaata tcctgatgaa attatattta
    10921 tctgaagacc tgttaccagc cacctgtctt ataccgtaac ccgatacata ccggatttat
    10981 tctatgaaaa agaaatcact tacactcctt ctgccgtcat tactcagcgg ctgtacggca
    11041 tttaatgcca cgcctgttcc tgacaccacg cctccggctc tggtgttcgt cgacggacaa
    11101 attcgcgaaa gcgctgccat tatcgcccat acgcaaaaac gtctgcttcc gccaccggtg
    11161 acccgccctg tgacgccgcc ccgcccgaca acaacagcga cgcccacaac cgcagtcagc
    11221 agccagagta acgcactgcc tccccgcccg ctccttcagg ccacacagct gcccgcactg
    11281 gttatgaccg gtaaacccgg tcagtcatcc gtcgtttatt tacgtgaggc acgtcagcgc
    11341 tccgtcagtg actggatacg ccagttgctg ccacccggct ggcagttccg gcaacagaac
    11401 gctgccatac cggcactgaa caaacgcctc cccgtctggt ccgttaatga ccagtggcac
    11461 cgggcactga atactctgct gaccgggcag caactgtacg gacatatgga ctggaacagg
    11521 aaaatactga ctgtcacgac cactgcagca ccgcctgttg acctcacggc accgcagggt
    11581 tcacagaaaa cagcggacac accacgtaat ccgttcagga gcacaaccgg aacggctgca
    11641 gaccaggcac aggtaccacc tgccgtcagc gcttcccggg ctcagagcac aacagggaca
    11701 tccggtacat ctgcgccccg cacaaccacg ccagccccgg tggtcacgaa accggtagca
    11761 ccaccacaga aggtcacacc gccacggcag tggaaaatgg aaaaagggac cacgctgaag
    11821 gacggactgg cccgctggac gtcgtccgaa aaatgccccc acggcacctg gacgctgcgc
    11881 tgggaaacgc ccgttaacta cagtatcgat gcacctctgg ttttcaccgg cagtttccac
    11941 caggcgctgg aacagctctt cgcactctac accccggtgg aaaaaccgct gtatgcccgg
    12001 accagcacag cacaatgtct gctgcgggtg acagatcgtc cacaggaata accttatggg
    12061 ctggttcatc gtgttcatca tggtcatcac gaccacaggc aatttctggt tcaccagtca
    12121 gctctcccgc agcgaacacc gtcatcaggc cgcagaaaat acacaacagg cagcaacgtt
    12181 cattcgttat atgaacgcca tcaatgatta cctgcatcag catccggaac gacggacagc
    12241 cggagggcgg ctgacatccg ctcagctcgg gattcccggg acggataccg tcagtcatat
    12301 catcagccag caacgtgttt ttgtctgggc tacagaaacc ccgggcctga tggccgcgct
    12361 cagggaacag agtaatgact ctgctctgct cggcagggtg gaaaacggcc gactgctgga
    12421 cactgccggc agagcgcttt ccgtcacact tccgtcagtg atccccgatc acgtcatcct
    12481 ctggatgaat tagtcacgaa tctgaataaa tcacggatct cccatgaata aaaaaattct
    12541 tgccattatg attgccgccg ctctgttaca gggctgtgcc agttacagac agcttgagag
    12601 cacaagaaag gaagccgttg cggaacaaca acgggcacgg gaaatgatgt ctgatctgcg
    12661 aacccggacg ccggttgtta aggaatcctc acgacaatgg atcaatcccc gtccggtcat
    12721 ggataaaaac accggcaaaa ccgttcccgg ttgtcccatt gtcattcata cccgtcagag
    12781 catcaccctt cagcaggtgg ttcagcggat cacggaaagc tgccatattc cggtacggat
    12841 cacacctgat gtactggcat acctgaatac atccacaacc ggcagcacac aacaaatcca
    12901 gggcgcgtta cccccaccgg atgcgagcgg catggttccc cttgccgcca ttggcgcatc
    12961 cgacacgcca ccatcccgtt tgccgggaaa taccgcagca ctgtctgaac tgaatgcctc
    13021 cggcctgacg accctgctga atgcggtctc ctcccgtctc ggtatatcct ggcgctatga
    13081 cggctcgcat atcatcttct attatctcga gacacagacc ttccccatca cctatatgga
    13141 cagtcaggtg gcctataacg ccactgtggt cagcggcacc atgtcctcaa acggctcatc
    13201 aggaggcagt gcatcagatg ccatttccgg tgatgccagc aatacccaga ccaccaccgt
    13261 ggatatgaag tctgcgctgt atcaggatgt gaaaaatgcg gtcacatcaa tgctgacccc
    13321 gggtatcgga cgcatgttca tgtccacggg ctttatcacc gtcacagata atccacaggt
    13381 tctggaaacg gtccgggcat tcattgaaaa acgcaatgaa gagatgaaac gtcaggtggt
    13441 gctgaatgtg gaaattctga gtatccggaa aacccgcaat gagcaggccg gtatcgactg
    13501 gaacgcagtc ttcagcgaca gaacgctggg actgagtctc ggcagcacat tcaccagtgc
    13561 agccagtgat gccatcacag gtggtgtttc cattgtggac ggtaaactca ccggttcaaa
    13621 agccttcctg aaagcattgt cttcccaggg ggatgtcagt gtggtgaccc agaactctgc
    13681 cgtcaccaaa aacctgacgc cggtgccaat gcagatcgcc aatcagcaga gttacataga
    13741 gagtgtgacg acggatacca ctgccaatgt cggctcatcc acctccctga atgcggccac
    13801 catcaccacc ggttttaaca tgacccttct gccgttcatt ctcccggact cacagacact
    13861 gcagttgctc tattccatga gtctctccga caaaccggtt attgagaatt atgagagcgg
    13921 tggaagtaaa gcccagctgc cgaatgtgga tctgaaaacc atcaatcaga ccgtggatct
    13981 gaaatccggg cagacggtca ttatcagtgg cttccagcag tcaggacgtc gcagcggaaa
    14041 acagggtgta ggtacgccgg gattcttcgg tcttggcggt gggatcaaca gtgaaaacga
    14101 cgacaccatt ctcgtggtgc tgatcacgcc caatatcatc tgatttgcac aaggaggacg
    14161 atcgtgacgg atatttcacc gacagggcgg caggtgtttc cgctgacggc agggcgcaat
    14221 gtgtttctgg ccggtctgga atggaaaaca ctgccctcac agtaccggca tgcccgcgac
    14281 tttgcccggg cacagaaagc cgaccttttc ctggcctgtc agtatctcag taatgaagat
    14341 gccgataccc acaccatggt agccactgtc agtcgccgca tactgcccgg aaaaccccgt
    14401 cagtgcttct ccctcgccct gctgatcctg ccactgcttg aacatggcgg ctatgccatc
    14461 acggaactga cgttacccgg agaaacaccg cgctatagct ttgtttccgt tgtcgacggt
    14521 gttctcgtca gtgacctggt gggaagcgga gaagaggttc gggaagcccg ggataccttt
    14581 ctttccatca acaccgaacc gactcagggc tggatacact atgaaccggc ggcattttca
    14641 gcaggcgatc agaatcagca cctgccgctg gcctccctga ctggctctgg taaacatccg
    14701 gctgccgcac gcctgaatcc ggtatcccgg ggaccgcagt tcatcactgt ctctctgatc
    14761 atcgcccttc tcggcactgc atggtacggc tggcagtatt atgaacgctg gcagacagaa
    14821 aaagccgcgc aggcagcggt tgcccgacag gccacagagg aacgaatcac tcccccctgg
    14881 cccggactcc ctgaaacaga cgcgttcatc cggggatgtg ctgatatatg gacttcactg
    14941 ccactgtctg ctgccggatg gcgttattcg ctggcagaat gctcgactga cggtggcagc
    15001 ggcaatcttc gcgcatcgta caccaatact gcgggcgcga cagtgactga cttcatccgg
    15061 cgcatcagtg atatgacaga cagccgaccg tttattgtga tgccggaagg aaagaccggc
    15121 ggcattggcc tgccggtcac cttccggctg tctgaaaacg ccgtcacagt tgatgcacta
    15181 ccggaaaccg ctgtcctgca ggaacgactg acctcgctgg ctcagtcaat gcaactgaaa
    15241 ctggactggc aggaagttaa taacagcgtc actgatgaga acggacagat cattcagccc
    15301 ccatggaaag agtatgacct tcagatacag accacactcc ctgcacacca tctggctgaa
    15361 cgcctgaaag agccttctgt ccgttttatc tctgtcacac ggcaactgga gaacggtcgt
    15421 ttccactatc aatttacagg taaatattat gcccgataaa gccaaaaccc ttctgttttc
    15481 agtcctgctg ttaccggcca cggcaatggc cacaaccgga aatgcagagc aacagaacac
    15541 acagcaaata aggattgaac agatacagga agagacagcc ctgtatgaag ccatgctggc
    15601 gagagagaag atccgccatg aactgcgaac acttgagcag ggcgttcccg cagaggccgg
    15661 acgtcagcag gaaagcattt ccgcagttac aacaaatgta caggcattac catccccccg
    15721 cctgacagca atattcggtg ttcatcctca gctgactgcc cggatagaac ttccgggggg
    15781 aagcatctcc gatgtgaagg cgggccagaa catccccgga accacttatc acgtggaaaa
    15841 aatcacgccg gacagtgtgg tgctggaaca gtctggcacc agaaaaatcc tgcgaccatg
    15901 agaccggccg ccgtaactgg cggctgtcaa aaataaaagg atgaaagaca tattttccgg
    15961 gcaccgctgc ccgtaaggag gctttatgct ggatccagca gacctgccgc cggatgacat
    16021 tctggattat gtggccatcg acaccgataa cgccggacaa ctgcggatca gggttgttga
    16081 gggaaaaaaa catctgcgtg ccgtccagga atatctgacc cgccttcgcg cacgctatca
    16141 ggggcgtgtg ggagactttg agttcaccac gctggatgtg atagcccgcc tcagacagga
    16201 cgccacaacg acaggcgatg aaaacgctat taacccgata cagcagaaaa tgctcggcta
    16261 tatccgccat tctgcctccc ttggtgcctc cgaccttcat atgacgccag ggcgggacaa
    16321 cacagacttc acctatgtgg aagcccgggt tcatggcgaa ctggaagtgc tcgatatcct
    16381 ccggaaagaa gaaggtctgg aattactggg ggctacctat tccggcatga cggatgtcat
    16441 caagggcaca caatttgatc ccggggttcc acaggacgca cgtctctctg aacagtacct
    16501 gaaactggct ggcctgtttg gcgcacgcta ctcccactac ccctgcgtgg gaggcctcta
    16561 tgctgttctc cgtctgatca aggatgacag ccagcaaatc ccgaccttca gtatactggg
    16621 ttatcacccg gatcaggagt gcaccctgcg ccgtatcctg caacgccctg aaggcattat
    16681 caccctctct ggccccaccg gctcgggaaa atccaccacg ctgagaaccg cctctgcggc
    16741 ataccttgaa caatacggct tcaataacac cggcggtatt cttctgccac ggcgcaggtt
    16801 gttcacgatt gaatccccgc cggaaggccg tatccccgga gcaattcaga cagccgttat
    16861 ggataccacc cggggctggg tggactccat caagtcagca ctgcgtcttg atcctgacag
    16921 catcctgaac ggtgaaatcc gcgatcacga ttccgccatt acggcaataa aagccgccat
    16981 gaccgggcat ctgatgctga ccaccatcca tgcgaacgac ccgattaaca tcctcgaacg
    17041 ccttgagatg gagggtgttc aggccaggat gattgcagac ccgcagttgt tcatcggact
    17101 gctcagtcag cgtctggttc agctgatatg tccccactgt cgtcatccat ggaatgacgt
    17161 agcatccagt cgtacaaagg atgagcgaca tctggtggaa accttctgta atgctgatgc
    17221 ggtatatctg agaaaccatg aaggctgtcc acactgctgg cgtggtgtca cagggcgtac
    17281 agtgattgca gaagtcatct ctcctgacgc cagatttttt cagttatacc gggaaaaagg
    17341 ccgtatcgaa gccaaaacct actggcacag ggaactgggc ggaatgacgc gtaatcagca
    17401 tctgctggga aaaatcaaca gcggactggt tgaccctctg gcggcccatt acatctctcc
    17461 cattgatgag gacagctata cgctgctgcg ctgatatgaa atttctggaa aaactcaaca
    17521 gtactgaact ggatctgtct gccctgaaca gctggacagc cagaaaaacc ttcggtctta
    17581 agcgtgccga gttctatgag tccctcggcg gaatgatacg tgacggaacg ccgtcactgc
    17641 gcgccctgga gtttatctgt aatgtcgaaa ctgactttgg cacccgaaaa ggaacatccg
    17701 gcatgtactt tctggccaga gaatgtattg cctccctgaa agccagcggt atgctgtcgg
    17761 gggccctgcc cacatgggtt cccccggaag aagcggcact gatcaggaac ggtgaagaac
    17821 gcgggaacat cgccgaagcc ctgttccagg tcgcaaaaac cgtgaaagca cggcgggaaa
    17881 tggtgagcag tctcatcaca ctctgtcttt atccccttct gctgctttca ctctgtgtgg
    17941 agaatatgta caacgcacat acccgcctca tccccattgt cagcgctttt gcaccggagg
    18001 agaaatggtc attccagatg aaagtccttg cagccatgtc agagcttgtc atcggctatg
    18061 gtctgtggat cagtggtgcc atcatcacgc ttgtcatcat catccgcttc tcactcagtc
    18121 gctataccgg cccgggacgc aggcttcttg acaaactgcc accatggtcg ctctatcaga
    18181 cttttcatgg tgtgaattat ctgttcagca tatcgtcaat gctgcagata aatattccgc
    18241 tttcacaggc gctgctcaga attgaaaaaa gtgcagagca taataaatgg ttaaaggaga
    18301 gaattcaggc aataagaaaa aatgtattat ccggtcagaa tctggccacc gccatgcgca
    18361 attctgacta tgattttcct tcaaaaatat gcatcaataa acttctttta aattctgaaa
    18421 gggaagacag cgtatctgca atgaatacct atgccggaca gtggctggaa gatgcaaaaa
    18481 atagcgtcag aaaaagtgga ttaattatca ccggtatttc aggaggtttt gtattctttt
    18541 ttattcttaa catgattagc gcgatataca gtatcagcga tatgctcaag caataatacc
    18601 tgccgtcata ctaaaacgcc cttcaatata ctttcattaa cgtaacaacc attaatacag
    18661 gagtcatata tgaacttcag agcacgtctg tcttcttatt tttctttaaa aaaagataaa
    18721 gaagatgata aaggtttaac attattagaa attgccggcg caatgattat catcgcaata
    18781 gttgtcgcgc tcgcgatgag ccagatgtcc agcacaatgg cctccagtga caaccagcgc
    18841 gaactgaaaa acctgcagac aattgcaacc aaaatgaagc aatacaagtt ccagggaaga
    18901 tataccggaa cggattatat caaaaccctg acggaaagcg gtgcattgcc tgctgatatg
    18961 attgcaggag gcaacaaagc caaaaacgca tggggtggtg atgtcacgat tgctgccaca
    19021 gcagacaaat atggctacat tatcaccagt aataatgttc ctaaaacaaa ctgtgttgag
    19081 ctgattaaca gcctgcgcag ttcgtccata tttaccaaaa ttatgaacac agccccgaac
    19141 acggtggatc ccgttaccgt ctgcagtagc gataaaaaca atatcaagct ggaaaccaac
    19201 agctaatgcc ctgccacaca gcactccctc cggggtgctg tttcccggag cacataatca
    19261 tgaaatggtt ttatctatca tctcttttct tattctgtct cccggcgact gccgccgtaa
    19321 acagtaacac actggatagc tgctttattc aggcaggaaa acgttactcc atcgatcccg
    19381 gactactgaa agtaattgcc cgtaaagaaa gcagcctgaa tccccgggcc atcaatcata
    19441 acagaaatgc cagcggaaaa attatcagca cagattatgg cctgatgcag attaattcca
    19501 cacatattcc cggactgaaa agaatgggaa ttatccgaag tgaaaatgaa ctgctcagta
    19561 atgtttgcct gaatatacag acaggtgcat ggatccttgc ccggcatttt cagcagtgtg
    19621 gtgtgaactg ggagtgtctc ggtagctaca acgccggatt taataaaaat aacactcacc
    19681 gcagaatgaa atatgcccgg ctgatttatg ccgcatatat gcagggacgt taataacgga
    19741 gaatacgagc catgttcctc atcatcttac tcgtgatctc cgctacgctg accatacagt
    19801 tacttgccat cagagtggcc ggacattatc tcatgcaggt gcataccctc cggcttccac
    19861 gtcacctctc cctgctctgt gttgttctgg cactcaccgc caccattacg cacatcatcc
    19921 gggggacaga cattaccgga ctttgctttc tgttgctgat ggcaggctgc atgacactga
    19981 tgacactaac tgacgcaacc tcctgccgtc ttccccgtcc cttcaccctc acattcctga
    20041 tgctgggtgt ggcgtttcgt ttatggcagg gcgatgctgc tattgcgctt gcctgtgctc
    20101 tgttctggtt tctcctgtta tctctcctga ggcgctacac ctcataccgc aagggagtgg
    20161 agacgctggg actgggggac gtgtttctga ttgcaggtgt ggctatgtgg tcatctgcca
    20221 acagcactcc gtggatcatc ggtattgccg ccaccggcac catggtctgt atgtcgctgt
    20281 tcccgcgcag taatacaggg gccgctatcc cgttcggtcc gtttatctgt gcatcactgt
    20341 acattttcac tttttttcct ggtgccattt ttcacaccgg agggtaacac catgcaaaaa
    20401 gacgacgata aaggttttgt tctgtttgaa attctggccg gactgattgt gatcggtatc
    20461 gccacaccaa tgatttattc agaaattgaa aactggctga atgagcagct ttaccagagt
    20521 gctgcatatc atgcagatgc ctacaatacc gcagccagaa actatattgc cgacaacaat
    20581 gcccgtctgc atagcggcag ccttccggca aatttcacgg ctgatgatct gattcgtcag
    20641 ggatatctga aacaggggtt taaccattct ccgtttggtc agagttatat cacgggtatc
    20701 cgccgtaacc agactacagg acgtcttgaa gccctgacct gctcaacagg ggggcagaat
    20761 atcaaagaag atggtctgcg cagtgtggca ggacagcttc ccgggctggg tggttatatt
    20821 ggtaaaaacg gaacagcaac cggcgcattc ggcgcctgga cagacaaacc gggggattac
    20881 ggactcacct gttcggcagg tcacattgcc gtcgtcatga cgggtgatga tctgcaggaa
    20941 agcgacaggt tgtatcgttt tcaggtcccg gggagaccag agctgaacca gatgcacacc
    21001 gccatcaaca tgggcggaaa tgacatcaat aacgccggta acctcaatgg tcagaaagcc
    21061 acggtgaaag gcgatataac cagcgaagac ggctggctta tcaccagaaa caacaagggg
    21121 tggatgaaca cgacgcacgg aggcggtttc accatgactg acagccagtg gatacgcgcc
    21181 gtcaataaca aagggatcac caccgatggt gaaatcaaag gaggcaaagt cagcggtgga
    21241 acgatccggt ctgacggtcg tctttccaca ggtgagtatt tacagctgga aaaaacagcc
    21301 accgcaggta cttcctgctc accggacggg cttgtaggcc gcgacagtaa aggggcaata
    21361 ctttcgtgcc aatccggtgt ctggaagaat aatatcagcg atgcactggt ttttgattat
    21421 tcggcctgtt atgacgcaga agaagactcc agaaaaccgg tttatgcgaa ctggggtagt
    21481 ggctcaaacg gctttgccgt ctgtgacggc aaagcaggct atggtgcggc aggattcaga
    21541 gcgtacagag gtaaaccggg gtattcggga agcatggtct gggtcaaaat acgttgctgt
    21601 aagttaaaag ttaatacgcc ttaaatgcat cagcttgctt tgacgatgta gttaaaggca
    21661 atgtttttgg ggcgcatttc cgttgtgctt ccctgggccc agtaaggcat accgctgtga
    21721 tgccctttca gccatccccc acgctcatga tagacaccgg attgatcggc ctgataactc
    21781 aggagatgtc tgttccgatc tccatccagc ccacgtccgt tgtcccagcc acggataaaa
    21841 acaccgcgca aatccggcag cttacacccg ggataagcac gggcaagctg aggataccgg
    21901 gaacatgaaa atgactgccc gttcatcacc aggtagcctt gtggaggggt ggctgatggc
    21961 cacggtatag ggcttccgac agggtaggat tcaaaacctg cccacatacc ggattggcac
    22021 gaaagtattg catttctgta tccagcctcc taccttcccc gtttttatcg tggaaggcag
    22081 atatgtttca aaaaaaaact cgcaaaatgt ccctcagccg ggcactggac aaatacctga
    22141 agactgtttc cgtccataaa aaaggtcatc tgcaggagtt ttatcgcgtc aatgtgatta
    22201 aacgtcaccc tatggccgat cgctatatgg atgaaatcac taccattgat atcgccggtt
    22261 accgggatca gcgtttagct caaattaacc cacgaacagg gcgtcaaatc accggaaata
    22321 ccgtccgtct tgaacttgcc cttttatcat cattattcaa tatcgcccgt gtggaatggg
    22381 gaacatgccg tatgaatcct gttgaactgg tccgaaaacc gaaaatcagt aatggccgcg
    22441 accgccgact gacttctggt gaagaacgcc gcctttcccg ctatttcagg gacaaaaatc
    22501 cgcagctcta cgttatcttc catctggctc tggaaacagc aatgcgccag ggagaaattc
    22561 tctcattacg ctgggaacac ctcgatttgc agcacggcgt ggcgcatctt cctgaaacga
    22621 aaaatggctc accacgcgac gtaccactct cccggaaagc acgtaactac ctgcaaattc
    22681 tgccacaaca gataaacggc aatgtgttca gttacacctc gtccggtttc aaaagcgcct
    22741 ggcgtaccgc tcttctggat ctgaaaatag aaaatctgca ttttcacgat ctccgccatg
    22801 aggccatcag ccgtctgttc gagctcggca cactgaacgt tatggagatt gcagccattt
    22861 ccggtcaccg ctccctgaac atgttgaaac ggtacacaca tctccgggca tatcacctgg
    22921 tcagtaaact cgatgcaagg cgtaaacaga ccagcaaaat ttccccttat tttgttccct
    22981 accctgcaac tgtcgggtgc agaaacgggc tgtttgtcgt gacactgcac gatttcgacc
    23041 ttgaaacccg tgcagaaacc cgggaactgg caatatctca cgccagcgta ttgttgctgc
    23101 gcgcactggc ccaggctgct cagcggggtg aacgcgtccc cacaccaggc gagctacccg
    23161 caaacattga tgaacgggtc atgatttgtc cgttaacaaa cagccagttc atgacaaaag
    23221 cgaacagtat tttcatatcc tcctcctgaa taaataattt ttgttcaaaa ttaacgctat
    23281 gagatccttt atgaaacact tatcctcccg tgcagccact gcggataata atcgtatcgt
    23341 tacaattgaa gacgtttaca caatcgcatt ctcgcaacca attcacaccg aatcagcgtt
    23401 ggggcaaagg tggatgttga tccatactgg tgaatatgaa agtgccagcg atgcaaaacg
    23461 cttccttgat acgctcgtca ccgggcactg tgcttgcatg gtaacaccat cacctgttct
    23521 ggatcatgac acctggctgc gtatccgcct gctgatgggc gagataaggg atgccaaata
    23581 aacattcatg gccccggaac ataaaaatag agccgggaac gccgggcact gcgtgctgct
    23641 cagcggagta aacacgttcc cacatcaggc gagctgcccg caaacattga tgaacgggtc
    23701 atgatttgtc cgttaacaaa ctgaaggcat atcagaatga aaataaacta ttgcttccgg
    23761 gttaaattaa tcacggtcat cctgtctcat ttcctgatac agatatatat gtggccagat
    23821 tttctgacag cgaaaaatat tccccgtctc attgccgcta tggtagctct gtacctgtct
    23881 gcagtaactg tcattccggc atttttactg tcagatgaca gtgagagatt taaaaacctc
    23941 tcacctttaa agcaaacaat attattagtt ctgtgtacag ttggagtaac atttttactc
    24001 ttacatggat ttaatacata aacaccttat ggaaaacccc tattatgtta taaataataa
    24061 aagactcaga ggataacacc catattgcga caagcactcg cattatattt acactttctg
    24121 taagttcact tatataaata aaaacaataa aaaaccacca cagagaaaca atcattaaag
    24181 agatacagta aacaaatata cacaacacac tcatcaccga ccagggtttc tccagcatga
    24241 caaaaatcat tgataccagc accacaatag ctgcattacc taacagagtt tcacgaataa
    24301 tattccttat aatttccagt ttttccttat gctgttttat tttcatacta acctcattga
    24361 ttttatcccg aaaaggaaac gatgatacac cagaatactt tcacggcgac tctgcagcat
    24421 aatgccagac cagcggcatg ctcagtcact tcctgtccga atccttttca ttcgccctta
    24481 aaacagagtt gcaaacactc ttgaaacaca ctcgatcata acgatcgaaa taactcgttt
    24541 ccgtttaaaa aacaaactct ctagactcaa tttatgaaca aaacgtgctg ccccgcatgg
    24601 aggtgaaatg aaacctgaca gtacaggtat tgccacccgc atgatgctct ccctggacag
    24661 agagcgaatt tgcgaatgcc tgctcagtaa tcgccagttg cagtccactc cccttcaggt
    24721 gcgttatccg cagggagtca gggatgccct gggtatcatg agcgaacagc tttctctttc
    24781 cgtctctgac ctgacgcgta tcctggtcga agaggcgtta agtgaaatgt ttctccctgc
    24841 agataacatc gtccgccgtc ttctgtgcag aatggaacac atcatgcagg cacatgatat
    24901 cagcgcaaca acgatggctg cattgctggc cccctggaat atccgccctg ccgtcttccg
    24961 ggagccggac agactcactg actatctgac cggggatatt cttgccgcac tggctgactg
    25021 gttttatctc agcccggaat ggctgaacgg cagggtgcat tatccgctgt atcgtccggg
    25081 ggactggcct gtcacacagg aggcattctg caggattatt tcttccggtg agaatgtgga
    25141 cattattctc tggcatggtt ttccgtttgc cggaacgcat tccggggaat attgcggtgt
    25201 tctgctcagg cagaaaaaag agattaataa tgccattatt tatccggtac tttcgttata
    25261 tccggtcaga atggatatgg aaaaagaagg ctggtttcag atggtacgaa aaatatctcc
    25321 ggatatccct gtacgcgccg taacgctcac tccggcacag gcagaatatc tgataacagg
    25381 gaaaatatta cccactgtgt tattcagggt ctcattatca ccctggtaat aaaactgagt
    25441 aataacccga aataaataac acaccaactt tttaatggca caaccgtgcc ctctgttgtt
    25501 atacccaaaa taactcaata caggaaactg aaatgatgat gaaaaagcat attacccgta
    25561 ctctcatcgc gtctgccgtt ttattttcct ttaattcgac tgcagcaacg tcatattttg
    25621 aagcccgcaa tgatgcgatg ggaggaacgg gggttgcctc ctctcactat ggtgtggcac
    25681 ctctggcaaa cccggccctg ctgacaaaag ccggtccgga agatgacttc agtcttctga
    25741 ttccgtccgt tggtgggcaa ttgtccgatc cgggtaatat cagtgacaac gcagacaaaa
    25801 tcagtgatga ctggaaagcc tttgatcgtg ccatcgacag tcagagcaac gtgtcgcaga
    25861 ctgctgcacg tctgaagacg cgacttcagg acttccgtca cacgcatgct gacgcacagg
    25921 tgggaacatc tgctgtggcg actcttcccg gtgacaccct cgctgccgct ctgatgctga
    25981 aaagctacgg tacggtcagt gtcgacggta aagtcagtga tgccgacctg aactatctgg
    26041 aaagcattgc aaacagtaac ggtcaggatg tggataaaga cagactgacc tctcaggctt
    26101 ttgcccgtgc cgcactggtc accgatgtgg gtattgctct ggctactgaa ctggaaacgg
    26161 ccggacaaaa gtggtctctg ggcttcacac caaaattcca gcgtgttgac ctgtttaact
    26221 acaacgtgct ggttaaaaat tacgacagca gcgctttcaa aggcgaccgt taccacaaca
    26281 ccagaaacgg cattaacgct gatatcgggg ccagcatgga cctggatgac aactggacac
    26341 tgggtctggt cgcacagaac ctgctccccc gcagtattga gacaaaggaa gtgaacggtg
    26401 tcactgaaac tttcaggatc cgcccgcagg tgactgccgg tgtctcctgg cacaacgaca
    26461 tgttcactac agcactggat gttgacctga ccccagccag tggcttcaca tcagacagta
    26521 aacgtcagtt tgctgccatt ggtgctgaat ttaatgcctg gaaatgggca cagttacgtg
    26581 caggctaccg ccagaatctg gccagcaatg atggcagtgc gttcaccgcc gggctgggta
    26641 tctcaccatt tgatgtcgtc catattgatg tggcgggtat agcaggcaca gacaacaact
    26701 acggtgccgt tgcacaactg cagttcacat tctgatcatc tctgccccgg tactgaccgg
    26761 ggtatccaga cctgcatcct cgcgttttcc gaccatcaaa cgacacagcc agacctgtag
    26821 tgctgcctct gatctggctg ttatctgaca tatcattttc gcatgaggaa atgcatgttt
    26881 aacaaaaata aaacgcatat cgaaaacaaa gcctttatgg tggaaccgtt ccgggatccg
    26941 acagctaacg aatgtaatgt cgtgatagca aacgatattt gtatccgggt agcactgtca
    27001 ccctctgagg tgctccagcc attcagcaaa aacacgaacc agtaaatcct ccccacaacg
    27061 ctttcttctt tcactgctca ggaaaaatac accatgaacc gtatgatacc tgcgggatgt
    27121 ctcgccgtac tctgcctgaa cggctgccat ctccccccgg ataatacagc tccgaccaga
    27181 aagtatggtg ctgcctctga tgtcgccgtc gcccggcatg cgcagtatca gctatggggg
    27241 cagggaattt actctgcagc ccttccggag cgtcagtccg gcggcagtct gaaggctcac
    27301 agcgaccgtg tttcctttga ctgggaaggc gatgccctgg aattgctcaa cgaactggcc
    27361 cgtctgcgcg gccagcagtt cagctacagc ggcgtccgtc tccccttacc cgtctcactg
    27421 cacgtccgcg acatgacctt tgctgatgtc cttcgtctca ctgaagccca gactgcctgg
    27481 cgggcaacac tctcgcagtc agccgggcgt ttacatctgt cttttatgac gccggagaaa
    27541 aaatgaaaac acacctgtgt ctcctgctgt ctgctctcct gcttacaggc tgtgccggac
    27601 agcatcaccg ttcttctgcc gtcatgacag aaaacgcacc gcccccggat atccgtgcat
    27661 ggctgacgcc ctccggcaac caggatgatg gtatttcaga tacccgtcgt cagatgctca
    27721 cggatgccgg cagaacgctg ggattccgtg gtggaaaagc acagcgctca tgggaactga
    27781 ttcaggcact gaatgcccgg gaaagtaccc tgaatgccct gtatgatttc cggcctctca
    27841 tcagcaagga aggctggctg cctcctgtta tcgatgaagc tcaggatgtt gcacacatca
    27901 gaccggacca gataagaaca gcctcccgtg tctggactat cctccgcccg gaacgctttg
    27961 tcagtaatcc acctggatgg cgtgactggc tgctgaaagg actctcaacc accgccacac
    28021 caggcactga aggccgggtt gtgccggaag accgggcaca acgcagacta tgggaaaacg
    28081 ccttacgtca gggatggcag gaagggcgcc tcaacgcaga tctcacactg gaagctaacc
    28141 agaagcgcct gacccgtgat taccggggca tgatgcttta cgcccttctg tggcgtcagg
    28201 gcatgatcac acgtccagat gtcacagagc agatgcagac ggttaccggc aacgggcaaa
    28261 aactcatcac tggcgatcgc gtccgccgcc tgaaaacaca tgctgaattt acgctgcaga
    28321 aatcgcgctg gcgtcctctt gtcagcaccg aaggagagaa aaatgatatc accagatgac
    28381 ttcggtacct tccccttctc ccgttttacg gcagaagagt ttcgccattt ttttgcctgg
    28441 tgtgcccgcc accgcgtcag tgatattgat ctgaccggcg gttcaccggt atccgtcagt
    28501 cgctttggac ggcgggttcg ctcatccgta aatccccttc cgtcctccat catgatggca
    28561 ctgagtgatg agctgtttgg gcgggaggtt cttccccggg ttctgtcagg tcatcccgtg
    28621 gatcgtaccg ttcaggtcag cggagatgcc tccggtcgct atggcctgca acgaggtcag
    28681 cgtgttcgcc tgcgctgcca cctgatacag ggaacatcgg caacagaaga aaaagccatt
    28741 tccgtcacca tgcgggtgat cccgtcagat attccggaca tcctcactat gaatattgaa
    28801 ccggatctgc tggatgccat gctcagaaaa accggcctgg gcttcatctg cggagaaacc
    28861 ggctccggga aatccacact ggcggcggca ctgtatcgcc acattctgaa aaatttcccc
    28921 gatcgcaaaa tagtgaccta tgaagatccg gtggaataca ttctgggcgg agagaatgac
    28981 cttctgcctc cccatcaggc cgaaatcggg cgcgatgtgg aaagttttgc tgcaggactt
    29041 cgctctgccg ttcgccgtaa tccggacatc attggtatcg gtgaaatcag ggattatgaa
    29101 acagcagatg ccgcagtcca ggcgggtaat accggacact tttgtatcgg caccatgcac
    29161 acaaaatcac ccggagaaac ctttgcccgc ctgctgggat tgtttcctcc tgaaatcagg
    29221 gattcgatgg ctgcagccac gctttccctc gtacagttca ttctggtgca ggtactggtc
    29281 agaacaaatg acggcggcag gcaggctgtc cgggaataca tcgtcatgac tgacgaactg
    29341 cgcaatacgc tctccggaca atcccatgca acatggggac attacattga cgaaatcatt
    29401 cgtcaggaaa aacggcggat aagggatcag gttctgacca tgtatcagaa cgaccgcatt
    29461 gagaaatcag aagcagcgct cttcatcccg gccggagagt tcccccgatg aaacgcgaag
    29521 gcttctggcg ggatgtaaca ccaccgctca acatttatgg tgtccccctg ccgtttacca
    29581 tgatttacct gctgttgttt ccgtatccgt caaaaacgac attctgggtg tgcagcggca
    29641 ttctgctgtt ttttatgcta ctgggatatt ccggctggac agtccgcacc ctctgtatac
    29701 gtctgtacag cctgcttcag ggtaagcagg catcgggacg tccctggtgg taccgacatc
    29761 gcacagaatt accccgtgaa tggcacggac cagaatagcc ctcttttgtc catcatcccg
    29821 gcgtatcaca acgccctcat atttaatccc cggagaacga catgaagtcc agatatcgta
    29881 cctggcttgc tgtcccgcct gaggagacag aagccgtaaa aaatgcagtc cccccgttaa
    29941 atggccgcaa agcggtagca tgggatccag agaaaaaact ctggtatgcc gaggcgggta
    30001 ccgacctttc actgctgaag cgctggttac cccgtccaca ggaaatgtcg atggatgccg
    30061 gcgatccggt cacagaattt cgtcaggttc tggaaaacgc cgggctggtt cttgagaaag
    30121 atccagaaat ggacggagaa atacacagag tgcccacacg ggatgacaga agaggctccc
    30181 agagtggcgc atacaaggcc tatcttgatg gccgcccctc cggctggtac cgcgattacc
    30241 ggagcgctga taacagtccc acaacctgga cattctcccg cgcagaaaat atcgatcctc
    30301 tcgccagact gcatctgaag gcatccgctc agcaaaatcg cgatgagaaa gaacgtaaac
    30361 tacagcaaca gtacaacaaa caggccggct atgcccgctc ctatcttaac gggttgcccc
    30421 aggcaacagc acatgaatac ctgacacgca agggcatccg ggccgcgcct ggcgtgcggc
    30481 tgaacaataa aaatgagctg gttattccct tcagcaacgg ccggggggaa atacgttcgt
    30541 accagcgtat tccggtcacc ggcgggaagg atgcccgcat tctgaaaggc tctgagaaaa
    30601 gcggcaactg gttcgccttc ggaaccccgg aaaatggccg tcccctgtta tttgcggaag
    30661 gttacgctac tgccgcctcc ctgcacgaag ccaccggcca tccggttctg atgacaattg
    30721 atgccgacaa tatgattaat gtggcaaaaa atgcccgcaa aatctggact gacagcccct
    30781 ttgtcttctg tgccgacaac gaccatcaac ggaaaattaa taaaggtgtt tcaagtgcaa
    30841 aaaaagctgc cgcaagcacg catggcgaag tcattattcc ggcctttacg gaggcagaaa
    30901 aagcacaggg actgaccgat ttcaatgacc tggacgccag ccggggacga gacgcctttc
    30961 agcatgtcat gaacgcacag ttaaaacata tcggtatccc cacacccaac agtgacattg
    31021 ccgaccaccg tgaagctgtc gtgatcggca acctgatatt cacaccggta aaagatgaga
    31081 agccacaaat gtcccctgaa aacaaacaga gcacagcccc ggaaacggtg ctggcgacac
    31141 cggatacgcc ttatgacatc cacactgaag agacagaaaa ccaggcatat gaagatgact
    31201 atgccgccta tgcatccctg cagtcagtaa ctacggagac gttatccccg gataaaccac
    31261 tggagaatat tgcgtctgcc gctgataccg gcacagagaa tacctctgcg gctaccgctg
    31321 gcgaccagga agacctcaat acagacgcca caggtacaga caccgctcac aacaccaaca
    31381 cagcagaaac aggaacgctt cctgaacctg cagagattca gcctgttcag aagtcctccc
    31441 ctgaaccaac agcagaagac aaacctcagg agagcgttat acctgccgct gataccggtg
    31501 aaaaaaatgc ctctgccacc accgctgaca accagagcgt acaggcagaa gagctctccc
    31561 caggtgacga cacaccggaa acctccgtct ccgacgcgac cgggacacag cacaccacca
    31621 caacgcagac tgaagaacaa ctgcaggtct ccatggccag cgctgacgac tggagcgcat
    31681 ttgcggaaga cctcaacgct gatgccactg tgatgaccac ggctcaccac accgatacaa
    31741 cagaaacagc ctcaccttct gaacctgccg ggactcagcc tgttcagcag gcaacccctg
    31801 aagcaacggc agaaaataaa ccgcaggaag aggatgttct gtctgcaacg gataccagtg
    31861 aaaagagtgc ctctgccgtc cccgataaca accgtgacgc accgcctgag gagctcaatg
    31921 ccgatgccac ggttgctgcc actgttcaca acaccggcac agcagaaaca gcctcacctt
    31981 ctgaaccagc agaaactcag ccagtccggc aggccaccgc tgaaccgacg gctgaagata
    32041 aaccgcagga agagcgtctt gtgtccgcca ctgagaccag cacagcgaat gaagcagctc
    32101 gccctgaaac gccatcgctt ttcagccgtc tgcgccgttt tctgaccggt gggaaatcac
    32161 gtacctcaga accttccgtt attcaggaga aaaagcccac ggaggccgaa actgcgacag
    32221 agactgcagc accaccgtca cctccggaaa ccagcccgga agacacatcg tcagcggaac
    32281 cggacacgat tatctttgcg ccccgccgtc ccgacagtcc gcagcggcac aacctggacg
    32341 agatccttaa aaatcttgaa cacaaagagt ttcccgacag gacagcgctc tacagcgttg
    32401 acggtgaacc ggcattcctg gacagactct actgcctcga aatggtggat ggcgccagcg
    32461 cggatgacaa aaaggttctg gctgcccttg ctgtggccac caacttctat ggcggcgtta
    32521 tcgagctgac cggcagtgat gcctttaaac aaaaagccat gcagctgatt gtcgaatacg
    32581 acatcaaggt tcgcatgaaa ctcccggccc agcgggcagc actggaaaaa ctccgcaagg
    32641 aaaaagagat ggtgcatgat gccattgtca cccacagacc cacgccggaa ctcaaccgcc
    32701 agtcaccgga agaaacaaca atggcacaac cggaaccccc tatgtcaccg aaggcagagg
    32761 ctgaacaaac agcaggctgt acggctgaaa caccgcatcc ggaagcgccg gtcacggcaa
    32821 aagctgaagc cccccgggcc gacagaccgg aacaaccagc cgccccgtcg tcagcgccag
    32881 aaaaagcccc tgtcagtgca cagccgcagc aacccgacac accggaggaa gagccgcccg
    32941 gcaagctcag ccccggtgaa agtgtgactg ccgtcctgac aaactacgga gaaaaagagt
    33001 atgcgcccgg aaaagggcgc tgcttttttg tggagctcac caaccgcagt ggcaaacggg
    33061 aatactgggg aaaaggcctg gaagaactgg tcaaaaacca ccagaaaggc gaccccgtca
    33121 cgctgacatt gcaggcccgg gagagatcac cttcatcacc gggcaaaccg gaatgggtgc
    33181 gaaatatctg gaccatgaaa ccggtctgta acgggatcag cgtatcgcat gataacccgc
    33241 atgaaggcca gtacatccgg gaatatccgg cggccacatt ccgccagatg atgaccctgt
    33301 tacagcaggg atggccacag ctcatgcagg atgtacgtct ccccgacaaa gtacccgaca
    33361 ccctgtatct gggggaggac cgccagcccg ccgctgcacc gcgagacccg gcactgcggg
    33421 ttccacttaa cggaacaccg cccgccacac tgacgccgct catcttctcc gtcgacaaac
    33481 aaagccagcg tctggatctg ctgctgttac aaagcgccga cgaatacatg cagggcgtgg
    33541 tccgcctgaa cggcaccctt tacccggcac tggccacccc gacagcggac agccgtcagc
    33601 tggtcatcaa cgccatgacc gacaggggcc ttcagtttgc cggttacggt gaggccatca
    33661 accgggatgc tgacggccct aagcgtcctg ccccacagtt gatgcagttt cacctgaagc
    33721 aacaggactc cccgttgttt gccgccatcc acaaaccgga agagcaaccg gacaagcttt
    33781 tccgctctct gggttttgag cagacctgga aagagtggtc tgacagccaa aaggcggagg
    33841 acaggcagga aaaaactctg caacaggcac aatcccacag ccccggcctg tgagccggta
    33901 aggacgacca tgaacatgaa aaaaacgctg ctggtcatac tgaccagcat ctcgctggct
    33961 tctccggctg ccgtagcagc aacctgtcgg gcaggtgctg ccgcagaagc cggcagcaaa
    34021 gccgcctggg agcgggcgcg cacagccagc caggcatggt cacagcgaga aaacgacgtc
    34081 gcctcctccc tgcagtcctg cctgtcccgc attaaggacg tcagcatcac tcttccgcaa
    34141 ttccccagcc tggacaaaat ccttcgcgat ctggaaaacc aggtctgtga tgccgtcgtg
    34201 gatcgggtga atgaacacct gccggggaat atcgacccct ggaaagacta taacctctga
    34261 acaggagccc catgagcgaa gcacacccca catcccctgt ctctccggaa acagataaca
    34321 gcggagatat ttataaacaa acgctcagta ccattgcaca gtcagaacaa cgggccgcac
    34381 tggttcccgg actggtcagg gccttgctgt gtagcagcgc agctctcgtc atcagtctga
    34441 ccggcaatat catacagtac tggcataaca gcaacgtaac gcgtgaatac tttgccaccg
    34501 acaatggtcg gctggtccgc ctggccccca ccagtcagcc agcctggagt cagagcgacg
    34561 tcatgacgtt tggcagcatg acgctgagcg aagcttttaa tctggatttt gtccactaca
    34621 ggaaacaaat cacgacactg gccccccgct tttccgaagc cggttttgcc ggctatgtga
    34681 atgctcttca ggcctccaac attctggaca ccattaaaaa ggagaaattt aacctcacag
    34741 ccaccatcgg ggccggcacg ctggtgaatc agggccagat ggaaaacggc atctggttct
    34801 ggaaattcca gtatccggtc aggatgcgtc tggtcggtca gaccagcacc cgccccgagc
    34861 aggccttcac ctttgaaatc actgtccggc gcgttgaccc acgaatcaaa cccggcggca
    34921 tggaaatcag ccagatggtt tcccgtaacg ccgcatcagg aacctgagac tcatatcatg
    34981 caaatacatc atcttcttgg cgcactgctt attgtgtgcg gcactgtcac ggctgcagat
    35041 aacccgggct ggcagaacgc acgaacccca caggctggcg gaaagacagg acaggctccc
    35101 gggaacgccc ccgcaggc
//
LOCUS       DAZGAN020000043        35074 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGAN020000043 DAZGAN020000000
VERSION     DAZGAN020000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.043"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            28..1235
                     /locus_tag="ACHKYO_RS11555"
                     /old_locus_tag="pgaptmp_002311"
     CDS             join(28..349,349..1235)
                     /locus_tag="ACHKYO_RS11555"
                     /old_locus_tag="pgaptmp_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4429152.1"
                     /translation="MTGILLGQELRKRKTPQEKIAIIQQTMEPGMTVSHVARLHGIQP
                     SLLFKWKKQYLEGSLTAVAAGEDVVPASELAAAIKQIKELQRLLGKKTMEVEILKEAV
                     EYGQSPKMDSACALVANGQGITLVSRILGVSRAQLAQRMKTSGNKPNKRRRRCDEAAD
                     AEVLARILDIISDMPTYGYRRVWAILRRQSRNKGVPFVNAKRVYRIMSENNLLLLHDK
                     PSRPQREHKGRISVKESDQRWCSDGFEFGCDDGAKLRVTFALDCCDREAIDWAASTGG
                     YDKATVQDVMLGAIEKRFGDKGAERPIQWLTDNGSAYTAHETRQFARELNLEPCTTAV
                     SSPQSNGMAERFVKTMKEDYIAFMPKPNVRTALHNLAVAIEHYNETHPHSALGYLSPR
                     EYRRQRVTST"
     gene            complement(1569..2753)
                     /locus_tag="ACHKYO_RS11560"
                     /old_locus_tag="pgaptmp_002312"
     CDS             complement(1569..2753)
                     /locus_tag="ACHKYO_RS11560"
                     /old_locus_tag="pgaptmp_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4429153.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(3053..3147)
                     /locus_tag="ACHKYO_RS11565"
                     /old_locus_tag="pgaptmp_002313"
     tRNA            complement(3053..3147)
                     /locus_tag="ACHKYO_RS11565"
                     /old_locus_tag="pgaptmp_002313"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3111..3113),aa:Sec,seq:tca)
     gene            3440..4822
                     /gene="yicJ"
                     /locus_tag="ACHKYO_RS11570"
                     /old_locus_tag="pgaptmp_002314"
     CDS             3440..4822
                     /gene="yicJ"
                     /locus_tag="ACHKYO_RS11570"
                     /old_locus_tag="pgaptmp_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HGU4429154.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            4832..7150
                     /gene="yicI"
                     /locus_tag="ACHKYO_RS11575"
                     /old_locus_tag="pgaptmp_002315"
     CDS             4832..7150
                     /gene="yicI"
                     /locus_tag="ACHKYO_RS11575"
                     /old_locus_tag="pgaptmp_002315"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HGU4429155.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(7203..8912)
                     /gene="yicH"
                     /locus_tag="ACHKYO_RS11580"
                     /old_locus_tag="pgaptmp_002316"
     CDS             complement(7203..8912)
                     /gene="yicH"
                     /locus_tag="ACHKYO_RS11580"
                     /old_locus_tag="pgaptmp_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU4429156.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINTEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(9033..10424)
                     /gene="xanP"
                     /locus_tag="ACHKYO_RS11585"
                     /old_locus_tag="pgaptmp_002317"
     CDS             complement(9033..10424)
                     /gene="xanP"
                     /locus_tag="ACHKYO_RS11585"
                     /old_locus_tag="pgaptmp_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HGU4429157.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            10704..11909
                     /gene="gltS"
                     /locus_tag="ACHKYO_RS11590"
                     /old_locus_tag="pgaptmp_002318"
     CDS             10704..11909
                     /gene="gltS"
                     /locus_tag="ACHKYO_RS11590"
                     /old_locus_tag="pgaptmp_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGU4429158.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            11912..12778
                     /locus_tag="ACHKYO_RS11595"
                     /old_locus_tag="pgaptmp_002319"
     CDS             11912..12778
                     /locus_tag="ACHKYO_RS11595"
                     /old_locus_tag="pgaptmp_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429159.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISVWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIHSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(12763..14844)
                     /gene="recG"
                     /locus_tag="ACHKYO_RS11600"
                     /old_locus_tag="pgaptmp_002320"
     CDS             complement(12763..14844)
                     /gene="recG"
                     /locus_tag="ACHKYO_RS11600"
                     /old_locus_tag="pgaptmp_002320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGU4429160.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPVEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(14850..15539)
                     /gene="trmH"
                     /locus_tag="ACHKYO_RS11605"
                     /old_locus_tag="pgaptmp_002321"
     CDS             complement(14850..15539)
                     /gene="trmH"
                     /locus_tag="ACHKYO_RS11605"
                     /old_locus_tag="pgaptmp_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HGU4429161.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(15546..17654)
                     /gene="spoT"
                     /locus_tag="ACHKYO_RS11610"
                     /old_locus_tag="pgaptmp_002322"
     CDS             complement(15546..17654)
                     /gene="spoT"
                     /locus_tag="ACHKYO_RS11610"
                     /old_locus_tag="pgaptmp_002322"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HGU4429162.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(17673..17948)
                     /gene="rpoZ"
                     /locus_tag="ACHKYO_RS11615"
                     /old_locus_tag="pgaptmp_002323"
     CDS             complement(17673..17948)
                     /gene="rpoZ"
                     /locus_tag="ACHKYO_RS11615"
                     /old_locus_tag="pgaptmp_002323"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGU4429163.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(18003..18626)
                     /gene="gmk"
                     /locus_tag="ACHKYO_RS11620"
                     /old_locus_tag="pgaptmp_002324"
     CDS             complement(18003..18626)
                     /gene="gmk"
                     /locus_tag="ACHKYO_RS11620"
                     /old_locus_tag="pgaptmp_002324"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGU4429164.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            18884..20566
                     /gene="ligB"
                     /locus_tag="ACHKYO_RS11625"
                     /old_locus_tag="pgaptmp_002325"
     CDS             18884..20566
                     /gene="ligB"
                     /locus_tag="ACHKYO_RS11625"
                     /old_locus_tag="pgaptmp_002325"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HGU4429165.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEDISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNARDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(20563..21180)
                     /gene="yicG"
                     /locus_tag="ACHKYO_RS11630"
                     /old_locus_tag="pgaptmp_002326"
     CDS             complement(20563..21180)
                     /gene="yicG"
                     /locus_tag="ACHKYO_RS11630"
                     /old_locus_tag="pgaptmp_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HGU4429166.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(21472..22296)
                     /gene="dinD"
                     /locus_tag="ACHKYO_RS11635"
                     /old_locus_tag="pgaptmp_002327"
     CDS             complement(21472..22296)
                     /gene="dinD"
                     /locus_tag="ACHKYO_RS11635"
                     /old_locus_tag="pgaptmp_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HGU4429167.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(22517..23380)
                     /gene="yicC"
                     /locus_tag="ACHKYO_RS11640"
                     /old_locus_tag="pgaptmp_002328"
     CDS             complement(22517..23380)
                     /gene="yicC"
                     /locus_tag="ACHKYO_RS11640"
                     /old_locus_tag="pgaptmp_002328"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HGU4429168.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            23507..24223
                     /gene="rph"
                     /locus_tag="ACHKYO_RS11645"
                     /old_locus_tag="pgaptmp_002329"
     CDS             23507..24223
                     /gene="rph"
                     /locus_tag="ACHKYO_RS11645"
                     /old_locus_tag="pgaptmp_002329"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HGU4429169.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            24289..24930
                     /gene="pyrE"
                     /locus_tag="ACHKYO_RS11650"
                     /old_locus_tag="pgaptmp_002330"
     CDS             24289..24930
                     /gene="pyrE"
                     /locus_tag="ACHKYO_RS11650"
                     /old_locus_tag="pgaptmp_002330"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGU4429170.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(24967..25563)
                     /gene="slmA"
                     /locus_tag="ACHKYO_RS11655"
                     /old_locus_tag="pgaptmp_002331"
     CDS             complement(24967..25563)
                     /gene="slmA"
                     /locus_tag="ACHKYO_RS11655"
                     /old_locus_tag="pgaptmp_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HGU4429171.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(25670..26128)
                     /gene="dut"
                     /locus_tag="ACHKYO_RS11660"
                     /old_locus_tag="pgaptmp_002332"
     CDS             complement(25670..26128)
                     /gene="dut"
                     /locus_tag="ACHKYO_RS11660"
                     /old_locus_tag="pgaptmp_002332"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGU4429172.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(26106..27326)
                     /gene="coaBC"
                     /locus_tag="ACHKYO_RS11665"
                     /old_locus_tag="pgaptmp_002333"
     CDS             complement(26106..27326)
                     /gene="coaBC"
                     /locus_tag="ACHKYO_RS11665"
                     /old_locus_tag="pgaptmp_002333"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HGU4429173.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            27498..28166
                     /gene="radC"
                     /locus_tag="ACHKYO_RS11670"
                     /old_locus_tag="pgaptmp_002334"
     CDS             27498..28166
                     /gene="radC"
                     /locus_tag="ACHKYO_RS11670"
                     /old_locus_tag="pgaptmp_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGU4429174.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            28383..28619
                     /gene="rpmB"
                     /locus_tag="ACHKYO_RS11675"
                     /old_locus_tag="pgaptmp_002335"
     CDS             28383..28619
                     /gene="rpmB"
                     /locus_tag="ACHKYO_RS11675"
                     /old_locus_tag="pgaptmp_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGU4429175.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            28640..28807
                     /gene="rpmG"
                     /locus_tag="ACHKYO_RS11680"
                     /old_locus_tag="pgaptmp_002336"
     CDS             28640..28807
                     /gene="rpmG"
                     /locus_tag="ACHKYO_RS11680"
                     /old_locus_tag="pgaptmp_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGU4429176.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            28905..29714
                     /gene="mutM"
                     /locus_tag="ACHKYO_RS11685"
                     /old_locus_tag="pgaptmp_002337"
     CDS             28905..29714
                     /gene="mutM"
                     /locus_tag="ACHKYO_RS11685"
                     /old_locus_tag="pgaptmp_002337"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HGU4429177.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(29753..30232)
                     /gene="coaD"
                     /locus_tag="ACHKYO_RS11690"
                     /old_locus_tag="pgaptmp_002338"
     CDS             complement(29753..30232)
                     /gene="coaD"
                     /locus_tag="ACHKYO_RS11690"
                     /old_locus_tag="pgaptmp_002338"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGU4429178.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(30240..31517)
                     /gene="waaA"
                     /locus_tag="ACHKYO_RS11695"
                     /old_locus_tag="pgaptmp_002339"
     CDS             complement(30240..31517)
                     /gene="waaA"
                     /locus_tag="ACHKYO_RS11695"
                     /old_locus_tag="pgaptmp_002339"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HGU4429179.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            31930..32988
                     /gene="rfaQ"
                     /locus_tag="ACHKYO_RS11700"
                     /old_locus_tag="pgaptmp_002340"
     CDS             31930..32988
                     /gene="rfaQ"
                     /locus_tag="ACHKYO_RS11700"
                     /old_locus_tag="pgaptmp_002340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HGU4429180.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            32985..34109
                     /gene="waaG"
                     /locus_tag="ACHKYO_RS11705"
                     /old_locus_tag="pgaptmp_002341"
     CDS             32985..34109
                     /gene="waaG"
                     /locus_tag="ACHKYO_RS11705"
                     /old_locus_tag="pgaptmp_002341"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU4429181.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            34102..34899
                     /gene="rfaP"
                     /locus_tag="ACHKYO_RS11710"
                     /old_locus_tag="pgaptmp_002342"
     CDS             34102..34899
                     /gene="rfaP"
                     /locus_tag="ACHKYO_RS11710"
                     /old_locus_tag="pgaptmp_002342"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HGU4429182.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYSADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         8395 a         8603 c         9318 g         8758 t
ORIGIN      
        1 atgtctaaca ctagtgctaa ttttgagatg actggaattt tgctggggca ggagcttcgt
       61 aaacgtaaaa ctcctcagga aaaaatcgcc attattcagc agacaatgga acccggtatg
      121 acggtgtccc atgtggcgcg tttgcatggt attcagccca gcctgttgtt taagtggaaa
      181 aaacaatatc tggaaggaag tctcactgct gttgctgccg gagaggatgt cgttcctgcc
      241 tcagaactgg ctgccgctat aaaacaaata aaagagttac agcgcttgct gggtaagaaa
      301 accatggaag ttgagatcct caaagaagcg gtggagtatg gtcagtcgcg aaaatggata
      361 gcgcatgcgc ccttgttgcc aacggacaag gaataaccct ggtaagccgt attctgggcg
      421 tgtcgcgtgc gcaactggca caacggatga aaacatctgg taataagccg aataaacgtc
      481 gtcgcagatg tgatgaggct gcggatgcgg aagtgttagc acgtatcctt gatatcatca
      541 gcgatatgcc gacatatggc tatcgtcgtg tctgggccat tctcagacga cagtcccgta
      601 acaaaggagt gccgttcgtg aatgcaaagc gcgtttatcg gataatgagc gaaaataacc
      661 tgttgttgtt acatgacaaa ccttcacggc cgcagcggga acataaaggc cgaatatcgg
      721 tgaaggaaag cgaccagcgc tggtgttccg atggctttga gttcggttgt gatgatggcg
      781 caaaactgcg tgtcacgttc gcactggact gttgcgatcg tgaagccata gactgggccg
      841 caagcacggg aggttatgac aaagcaacgg tgcaggatgt catgttagga gcaatagaaa
      901 aacgctttgg cgataaagga gcggaacggc caatccagtg gctgactgat aacggttcgg
      961 cttatacagc tcatgaaaca cggcagttcg ccagagagtt gaatctggag ccctgcacga
     1021 cggcagtcag tagccctcag agcaatggaa tggcagaaag gttcgtgaaa acgatgaaag
     1081 aagactacat cgcgttcatg ccgaaaccga atgtaagaac ggcattgcat aaccttgcag
     1141 tggcgatcga gcattacaat gaaacccatc cgcatagtgc gttaggttat ctctcaccaa
     1201 gggaatatcg acgtcagcgg gtaacgtcaa cttaagatac aacgactgtc tgaatatcgt
     1261 gggtcaagga cactttcctt gagaacaata attttatctt tatgtgaatc tcatttttta
     1321 taatgatgtc agatgattga aatgctaaat tatatctgaa tcaacttttc ctttgctaaa
     1381 ggtatcgaaa taactgcaag gctgtatttt gtaattgcca atttaaaaat gtcaatttgc
     1441 ttttaattat gtttttgtaa taagtttgaa gacgcaaaaa ataatataat aacaaaaatc
     1501 ctccagcctt gtaggccaga ggataaaata cttaattgag gaaaacatca gggaaaagta
     1561 tttctgactt aactttcccg gctggagagt gaatcaatgt aatcagcgta ccattgcatc
     1621 atttcacggc ggccatcgag atacagggca tgattatagg tacccctgat ggaattttta
     1681 tccacatgag ctaactgcat ttcaatccag gcggaattaa agccttgttc atgcagaatt
     1741 gtactcatgg tgtgtctgaa accgtgccct gtgagtcttc ccttatatcc aagtaattca
     1801 attacttggt taatgctttc cttactaatt ggtttgcggg gatcatttct accgataaag
     1861 actaggggat gatgttttga gattggttca agctgcttaa atagcataat gacctgctca
     1921 gagagcggca caatatgagg tcgcttcatc ttcattactt ctgccgggat ctcccacaat
     1981 tttgtctcaa aatcgatatc ttcccagcgt gcaaaacgca gttcttgtgt tcgtacgccg
     2041 gttaacataa tgatctgagt agctgtctta gtgattatac ttccggtata acccgccaga
     2101 tcgttgagga agtggggaag ttcattagca gtaagaaagg gaaaatgtac ttttttcggt
     2161 gtagctaaag cactggcaag atcgggggct gggttatagt ctgcccggcc agtaacaatt
     2221 gcatagcgga acacctcacc acagcgctgg cggaccttgc gcattttctc cagagctcca
     2281 cgtttttcca ttttacgcag tgcttccagt aactccattg gtttgatttc ggcaataggg
     2341 cgcttgccaa tgtagggaaa aatgtctttt tcaaaggtat caatgatttc atcacgatag
     2401 cgtaatgacc accgatcggc ttttgactgg tgccattcgc gagcaatagc ctcgaacgta
     2461 tttttcagac tcatttgctg cgcgattttt tcttcctttt ttgcctcccc tggatcgcct
     2521 ccagcagcga ggagtttcct ggcttcactg cgtttctcgc gagcatcggc tagcgttaca
     2581 tcggggtata cgccaataga aagcatcttt tctttaccag caaagcggta cttcatgcgc
     2641 cagtatttag aaccattagt gttaatcagt agatacatac cgccgccatc agcaagctta
     2701 tagggtttgt ctttgggttt tgcggttttc actttgatat cggttaatgc catgacatcc
     2761 tccggctttt gatgaattca tgttcctgag ggggatttct gccagtaact aacctgatac
     2821 ccccaataca ccccttgtat aacaaagatg ttgttaaaag ccgttgttat atatcacatt
     2881 tttcctatta tgggggtgct ggtatttcaa taggaaataa caccccctaa agtaccccca
     2941 aaagaaactt gatacggggt gaagtaggtt gacctctgga tacaaataaa tactgatata
     3001 caagggatta gaagagattt attgacattg ggagagttca gaagaggtaa actggcggaa
     3061 gatcacagga gtcgaacctg cccgggaccg ctggcggccc caactggatt tgaagtccag
     3121 ccgcctcacc ggagacgacg atcttccgcg cctcgattgc tacatggagg cggggcgcat
     3181 tatagctact tccttgagtt tctacatccc ccagatcagt tttttccgcc taatacacgc
     3241 agcgtattat cacgaaagca ttatagttca tataaaaccc atattttaca atgatatatg
     3301 agcggaatta acgctaccac gatcacataa aacaagaatc ataaattaat aaccagatat
     3361 cggaatattc gctctcacag ggatggttta caaaatgcgt tatcggtctc agcaccccta
     3421 tagcatcaag gaaaagcaga tgaagagtga agtgttgtcc gttaaagaga aaattggtta
     3481 tggcatggga gacgccgcca gccacattat tttcgataac gtaatgttat atatgatgtt
     3541 cttttatacc gatatttttg gcattcctgc cggttttgtc ggaaccatgt ttttggtcgc
     3601 tcgtgcactg gatgcgattt ccgatccttg catggggttg ttggccgatc gaacgcgctc
     3661 tcgctggggt aaatttcgtc cgtgggtact gtttggcgca ctgccattcg ggatcgtctg
     3721 tgtactggcc tatagcacgc cagatctcag tatgaacggc aaaatgatct atgcagcaat
     3781 tacttacacc ctacttacct tactttatac cgtcgtcaat atcccttact gcgcattggg
     3841 tggtgtaatc accaatgacc cgactcagcg tatctcgctg caatcctggc gttttgtgct
     3901 ggcgaccgcg ggaggcatgc tttctactgt tctgatgatg ccactggtta atttaattgg
     3961 cggtgataat aaaccactcg gtttccaggg cggtatcgcg gtcctttccg tggtggcatt
     4021 catgatgctg gcattttgtt tcttcaccac taaagaacgc gttgaagcac cacctacaac
     4081 aacgtctatg cgggaagatt tacgtgatat ctggcaaaac gaccagtggc ggattgtcgg
     4141 tttactaacc attttcaata tcctggcggt gtgcgtacgc ggtggggcga tgatgtatta
     4201 cgtcacatgg attttgggca cgccggaagt gtttgtcgct tttctcacca cttattgcgt
     4261 gggtaacctg attggttccg cactggcaaa acctctgacc gactggaaat gtaaagtcac
     4321 tatcttctgg tggacgaacg ccctgctggc agtgattagc ctcgcgatgt tctttgttcc
     4381 catgcaggcc agcatcacta tgtttgtctt catcttcgtg attggtgtgt tgcatcaact
     4441 ggtgacacct atccagtggg taatgatgtc cgataccgtc gactacggcg agtggtgcaa
     4501 tggtaaacgc ctgaccggga tcagttttgc tggcacgctg tttgtgctca aactggggtt
     4561 ggccttcggc ggcgctctta tcggctggat gctggcttat ggcggatatg atgcggcaga
     4621 aaaagcgcag aacagcgcca cgattagcat cattattgcg ctattcacga ttgttccggc
     4681 gatctgttat ttgctgagcg cgattatcgc taaacgctac tactcactca cgacgcacaa
     4741 tctgaaaacc gttatggaac agctggctca gggtaaacgc cgttgccagc aacaattcac
     4801 ctctcaagaa gtgcagaact aaggaacggc aatgaaaatt agcgatggaa actggttgat
     4861 tcaacctggc ctcaatttga ttcacccgct tcaggtgttc gaggttgaac agcaggataa
     4921 tgaaatggtg gtctatgctg ccccccgtga tgtgcgtgaa cgtacctggc agcttgatac
     4981 gcctttattt acgttgcgct ttttctcccc acaggaaggt attgtcggtg tgcggattga
     5041 gcattttcag ggggcgctga ataacggtcc tcattatccg ctcaatattt tgcaggacgt
     5101 gaaggtcaca atcgaaaaca cagaacgtta tgctgagttt aaaagtggca acttaagcgc
     5161 gcgtgtcagc aaaggtgagt tctggtcact ggattttctg cgcaacggcg aacgtattac
     5221 cggtagtcag gtgaaaaata atggctacgt gcaggacacg aataatcaac gcaattatat
     5281 gtttgagcgg cttgatcttg gcgttggcga aacagtttac ggtctgggag agcgctttac
     5341 tgccctggtg cgcaatggcc agacggtaga gacctggaac cgggacggcg gcacaagtac
     5401 tgaacaggcg tataaaaata tcccgttcta catgactaac cgtggttatg gggtactggt
     5461 caatcatccc cagtgtgtct cttttgaagt gggatcggag aaagtctcca aagtgcagtt
     5521 cagcgttgag agtgaatatc tcgaatactt tgttatcgac ggcccgacgc cgaaagcggt
     5581 acttgatcgt tatacccgct ttactggtcg tccggcgctg ccgcccgcgt ggtccttcgg
     5641 cctgtggcta accacttcat ttaccaccaa ctacgacgaa gcgacggtaa acagctttat
     5701 cgatggtatg gcggaacgca atctgccgct gcatgttttc cactttgact gtttctggat
     5761 gaaagccttc cagtggtgcg attttgagtg ggacccgctg actttccctg acccggaagg
     5821 gatgatccgc cgcctgaaag cgaaagggct gaaaatctgc gtctggatta acccctatat
     5881 cggtcaaaaa tcccccgtct ttaaagagtt acaagagaaa ggctatttac tcaaacgccc
     5941 cgacggttcg ctatggcagt gggataaatg gcagccaggt ctggcgattt atgactttac
     6001 caatccggat gcctgcaaat ggtacgccga caaactgaaa ggtctggtcg cgatgggcgt
     6061 tgattgcttt aagaccgact ttggcgaacg tatcccaact gatgttcagt ggtttgacgg
     6121 ttccgatccg cagaaaatgc ataaccatta tgcgtacatc tacaacgaac tggtgtggaa
     6181 cgtgctcaag gacaccgttg gtgaggaaga agctgtcttg tttgcccgct cggcctccgt
     6241 cggtgcgcag aaattcccgg tacactgggg tggcgattgt tacgctaact acgaatcaat
     6301 ggcggaaagc ctgcgcggtg gtttgtctat tggcctttca ggttttggct tctggagcca
     6361 cgatatcggc ggctttgaaa ataccgctcc ggcgcacgtt tacaaacgct ggtgcgcgtt
     6421 tggtttgctc tccagccata gccgtttaca cggtagcaaa tcttatcgtg tgccgtgggc
     6481 ttacgatgat gagtcctgtg atgtggtgcg cttcttcacg caactgaaat gccgcatgat
     6541 gccgtatctg tatcgtgaag ctgcgcgtgc gaacgcgcgg ggtacgccga tgatgcgggc
     6601 catgatgatg gagttcccgg acgatccggc ttgtgattac cttgaccgtc aatacatgtt
     6661 aggcgacaac gtgatggttg cgccggtgtt cactgaagcg ggcgatgtgc agttctacct
     6721 gccggaaggt cgctggacac acctgtggca caacgatgaa ctcgacggta gtcgctggca
     6781 taaacagcag cacggcttcc tgagtctgcc cgtttatgtg cgtgataaca ctctactggc
     6841 gctgggcaac aacgatcaac gtcccgatta cgtgtggcac gaaggcacgg cattccacct
     6901 ctttaatctg caagacgggc atgaagccgt ctgtgaagtg cccgctgctg acggatcggt
     6961 gatctttact ttaaaagcag cacgtactgg caacacgatt actgtgactg gtgcgggcga
     7021 ggcgaagaac tggacactgt gcctgcgcaa tgttgtgaaa gtaaatggtc tgcaagacgg
     7081 ttcgcaggct gaaagtgagc aggggctggt ggtgaagcct caagggaatg cgctgacaat
     7141 tacgttgtaa tctattgatg gaatgccggg tacgtctgag tcgtacccgg cgagattgat
     7201 gtttactgca ccagctctgc cgacgtaact tcaccgctgg aaacgatgcc agcattcatg
     7261 gtttgagtca cttgctgctt tgcggcgttc acggcatgca attgcccgct aaccgtaggt
     7321 ttcagcgggg cattggcctg aatatctcca ctggcggtaa gctgaatatt gccgtcgcca
     7381 gtcaacggta attcaggcca tccccattgt tgcaaaatat ttaccggcac accgcgtcca
     7441 ttcaggctga tatgggtctg acgttgtggc gtttgtgaaa cactggcagt ggcttccaga
     7501 atgccttttt cagtaaatgc actcagttcg ctgatattca ccgtgctgct gttggcggtc
     7561 agcgccagcg acgggcgacg aacatcaaca cgattgaatg tcgcggcggc ggcattcaga
     7621 ttcgccgagc cactccagac gccccattta tgatcggtaa ccagcgtcag gttggcaccg
     7681 taaccatcga gcgcggtgag ctgccacggg aagtcagggt cgatatcaat gatcagattg
     7741 cggctggcgc taaatctctt cagttgcagg ctgtttaacc aaccgggtgt cgtttccatc
     7801 cacaactgtt gccagttttt cggcaaggta tattccagcc cggcaattgc cgcatcatca
     7861 aggatcaacg ttttcccgtc acgcagccag ttccctgacg ttctgaccat acccccttcc
     7921 cagcggctgg tgaactggcg cagcgctacg ccctgcgggg aaaattccgt gtttataatc
     7981 gggtcaaata aatgcagcga accataaatg aactcgctag cgttcatcga cagtttgcct
     8041 tcctgtgtct gccagtcatc tttactgaag gtcatgttgc gcaagctgag atcgaggtcg
     8101 gtcaccgccc agtccggacc ttgcaaacga gcatcgatca cttccaggcg accaatctgc
     8161 aacgacggga cagagcgtaa tggcgcaaag aagtcggtta gcgatttttc gctttgtaga
     8221 cgtatatccg ccatgcgcag gttttccact tgccagctgc cgtcggcgtt acgctgcgcg
     8281 tttccggtta atgtcccgcg ggcgatgtcg gcacccaggt tagtcagcgt aacgcgatcg
     8341 ttatcaatac tgccttcaat cagtacattg gtggcaggaa catcattgag cgaaagcgat
     8401 ccggcactaa actgaatctg cgccttcgta cccagcactt taccggcttt tggtgaccac
     8461 ggaactacgc cgccatttac ccgctgcgcg ctcagtttcc attcgctatt cgggctatta
     8521 aacgccatat cacgcagttg cagacgatcg gctttgaacg gtagcggcgc ggtctggtcg
     8581 gtgagattca gcgtcccgtt ttccagcagg atggtatcga catggcgtgg ttcggtcagt
     8641 tgccgactgc ttagcgcaat gtcgacactt tttgccacca gggtcgcggg ctggccatca
     8701 cggccaaacg tgacgttctc cagcacgata tgagatggcg cggaaaaacg gtgatccatc
     8761 gccccgaagg ccagatgata gtcgctattc tcggaaaccc atgcgctgat atgttctgct
     8821 ccccagcggg tttgcagaag aaaatagagg ccagcgatca ccactaacag agcgatgaga
     8881 atgtagagaa gcagcttccc aataaatttc atggtcttcc atcccgtgaa atgcacataa
     8941 gggagttatg cacgatttac gcgcaatcct caaggcggga atggtgaaag aggtgagact
     9001 gcggcggtaa ttatcttacc gccgccagtg aattactgtt tttctggtgg gaaaatcaga
     9061 ttcagcacga tggcagtaat accgcccgcg gcgatcccgg aggagagcag gtttttcagc
     9121 cattcagggg caaactgcaa aatcagcggc tgctgagaca cgcccagacc aaccgccagc
     9181 gacagcgcga taatcagaat cgcccgacgg ttcagcggtt cacgagaaac gatacgcaca
     9241 ccggaggcgg cgatagtgcc aaacattaca agcgttgcgc cgcccagaac cggttctgga
     9301 atgtgttgta caaaaccgct cactgccggg aacagaccca gcacgatcaa catcagcgcg
     9361 acgacaaaac cgacatagcg gctggcaaca ccagtcaact ggatcactcc gttgttttgc
     9421 ccgaagcagg agttcgggaa ggtgttaaac accgccgaaa caaacgagtt caggccgttt
     9481 gccagcacgc cgcctttcag gcgtttcatg tacagcggac cggacactgg ctgttcggaa
     9541 acgtcagagg tcgccgtgat atcgccaatg gtttccagcg aagtgatcat aaagaccagc
     9601 atcagcggca gcagcagact ccattcaatg ccaagaccgt aatagagcgg cgttggcacc
     9661 ataatcagtt cttgcgtcat cggttcgttg ctttctggca acatgcccat aaaccacgcc
     9721 agcgcatatc cggccgccat cgcaatcacc agtgaggcca cgcgtaagta agggttacgt
     9781 tgacggttaa gcaggataat taaggctaag accacgcctg ccagcagcag atttttcggt
     9841 gcgccgaagg tgttatcgct catggctgcg taaccgccgc caatggacgt taacccaacc
     9901 tgaattagcg acaggccgat aatcatcacc acaacgccag aaaccagcgg cgtaataatg
     9961 cggcgcgcca gatgcagaac gcgggagatc accatctcgg tgcaacttgc cagcatcaac
    10021 gtgccgaaca aagccgccat catggtagga acatcagcac caccggtttt cagcgcggta
    10081 ccgcccataa tcagcggggc aacaaagttg aagctggtgc cctgaataga caacagcccg
    10141 gagccaaccg gaccccaggc cttaatttga ataatcgatg ccacaccgga ggcaaacagc
    10201 gacatactaa taatgtgttg cgtgtcttgt gccggtaaac ccagcgcctg gcagattaat
    10261 agcgctggcg tgatcaccgc aacgaacatc gccagcagat gctgacaggc ggcaaacagg
    10321 gtttgaggaa gcggcggacg atcttcaaga cggtaaatca gttcgctgtt ttgagtctgc
    10381 gcaaccggtt gcgcattttc tgactcgagg gtggaaacag acatcgaaaa caatcccgtg
    10441 gtagcaaagc gggcatttta gctgaccgaa agagaaaagc aaacgtttgc cacatcctca
    10501 tctaaatatt taaatgagat aaatgcactt ttttatataa cttttggtat ttttctgctt
    10561 aaaatccatg ccatttgttc attaacagct gagaatttga cccaggaaag cataactcga
    10621 tacccgtcat agttcatgaa gttgccactg cgcgatgaag tatgacgagt atgaaagagt
    10681 gatgcggata caaaggagta actatgtttc atctcgatac tttagcaacg cttgttgccg
    10741 caacgctgac gttgctgctc gggcgtaagt tggtccattc cgtctccttt ttgaagaaat
    10801 acaccatacc ggaacctgtt gcgggtggtt tgttggtggc gctggcgcta ctagtactga
    10861 aaaaaagcat gggctgggaa gtcaactttg atatgtccct gcgcgatccg ttaatgctgg
    10921 ctttcttcgc caccattggc ctgaacgcca acattgccag tttgcgtgcc ggtgggcgtg
    10981 tggttggcat cttcttgatt gtggttgttg gtctgttggt gatgcaaaat gccattggca
    11041 ttggtatggc tagcctgtta gggcttgatc cgctgatggg gctgttggcc ggttctatta
    11101 cgctttccgg cggtcacggt acgggcgctg cgtggagtaa attgttcatt gaacgttatg
    11161 gcttcaccaa tgcgacggaa gtggcgatgg cctgtgcaac gttcggtctg gtgctgggcg
    11221 gcttgattgg cggtccggtg gcgcgctatc tggtgaaaca ctccaccacg ccgaacggta
    11281 ttccggatga ccaggaagtc ccgacggcgt ttgaaaagcc ggatgtggga cgcatgatca
    11341 cctcgttggt gctgattgaa actatcgcgc tgattgctat ctgcctgacg gtggggaaaa
    11401 ttgttgcgca acttttggct ggcactgctt ttgaactgcc gaccttcgtc tgtgtcctgt
    11461 ttgttggcgt gattctgagc aacggtctgt caatgatggg cttttaccgc gtctttgagc
    11521 gtgcggtatc cgtgctgggt aacgtaagcc tgtcgttgtt cctggcgatg gcgctgatgg
    11581 ggctgaaact gtgggagctg gcttcgctgg cgctgccgat gctggcgatt ctggtggtac
    11641 agaccatctt catggcgttg tatgccatct tcgttacctg gcgcatgatg ggcaaaaact
    11701 acgatgcggc agtgctggct gcgggtcact gtggttttgg cctcggtgca acgccaacgg
    11761 caatcgccaa catgcaggcg atcactgaac gctttggccc gtcgcacatg gcgtttttgg
    11821 tggtgccgat ggtcggtgcg ttctttatcg atatcgtcaa tgcgctggtg attaagctgt
    11881 atttaatgtt gccgattttt gccggttaaa aatgaaaaag cgtcggaagg aaccggaaac
    11941 gctacgtgaa cactgtcgcc atatttttgg tgatgagccg ccagtgctct gtgtatggga
    12001 aacggaattt gattacgcag atgccgaact caaggcgctg gcggctaaag agtggcagca
    12061 gatcagcgta tgggatttga gtgcttacta tgtactcaat ctcgtttata acgaaccgat
    12121 gcaaatagag ttgtttcgct atctgtttcc gctctgcctg gcgcaatggc atgaaaccgt
    12181 tttagctggc ggttatggtg atcacttcga agaatcctta atgaaggcat tgtgtcggcc
    12241 ctatctctgg caggagatga tgaatgcttc ccaacgccag caggtacggc aatttttact
    12301 ggacaccgcg ttgcagcgta tggataacga gcgtggtttc aataacgttc tttgctggct
    12361 ggcggtgttc aatactctgg gtggggccgc gccgttaatt cattctctct ggtcgcgctg
    12421 gtgggcgctg gatacgccag gaaaagcggt gtgtgctatt cagtatgccg cgcatctaat
    12481 ttatcccata gaagccaatc cgttatggtc gcaagaatgg ataggctggg ggcatccatt
    12541 aggccataaa gatggctggt cctctgataa tcgcgctttt cttcgccaaa tgttaacacc
    12601 tgaaatgatt gtggctggcg tacaggcagc ggctgaaatt ctgcgtggtg aaccggaagg
    12661 cgcaatggcg gcgcgaattg cacaggatgc gtatgaggca atggatatcc tcactatcca
    12721 gattgaagat ttactgcgtg acttgtcttg tgatgagtct ggtcatgcgt tggagtaacg
    12781 ttccgtctcc ggcatccagc gttctatcag ggcttttgct tgttgtgggt aacgttcgtg
    12841 aatatgtcgc gccaggcgct gaacttccgg gatcatcgcc tgatcgcgca gtaaatccgc
    12901 cactttaaac tcagcattac ccgtctgacg cgtacctaac agttcacccg gtccgcgaat
    12961 ctctaagtct ttctgtgcaa tcacaaaacc gtcgttgctg tcgcgcagca cttgcagccg
    13021 aatttgcgcg gttttggaga gcggcgtttt gtagagcagc acgcagtgag aagccaccgc
    13081 accacgacct acacgcccgc gaagctggtg taactgcgcc agacccagac gctccgggtt
    13141 ttcgataatc atcagactgg cgttaggcac atcaacgccg acttcaataa cggttgtggc
    13201 aaccagcagg tgtagctcac cttgtttaaa cgacgccatc accgcctgtt tctcgacagg
    13261 tttcatccgc ccgtgtacca ggccaacgtt caactctggt agcgccagtt tcaactcttc
    13321 ccaggtagct tccgccgcct gcgcttccag caattccgac tcttcaatca acgtacaaac
    13381 ccagtatgcc tgacgacctt cagttatgca ggcgtggcgc acgcggtcaa tgatgtcggt
    13441 acggcgggta tcaggaatag cgaccgtagt cactggcgtg cggcctggcg gcagctcatc
    13501 tatcaccgag gtatcgagat cggcatacgc agtcattgcc agcgtgcggg ggatcggcgt
    13561 ggcggtcatg atcaactgat gcggatggaa gccctgctgc tggcctttct cccacaatgc
    13621 cagacgctga tgcacgccaa aacgatgctg ttcgtcgata atcaccagcg ccaggccgtt
    13681 aaactgcacc tgttcctgga agatggcgtg tgtaccgaca atcatctgca cctgaccgct
    13741 ggcgatggct tcctgctgtg ccagccgtgc tttacctttc tgcttaccgg cgagccagcc
    13801 cacttcgata ccgagcggtg caaaccagtt gcggaagtta ttggcgtgct gctcggcgag
    13861 taattcggtt ggtgccatca atgctacctg tttgccgtgg gcaatcgcac gcaacgcggc
    13921 gagggcggcg accagcgttt taccggaacc tacatcgccc tgcaccagac gcatcatcgg
    13981 cacatccagc gccatatcgc gctcgatctc cgccactacg cgtgcctgtg cgcccgttgg
    14041 cttgaacggt aaggcggcga ggagtttatt tttcagcgtg tcattggcgc tcagcggctg
    14101 ggcatgaaaa cgctgtgctc cggcacgtaa ggctaacatg ctgaggttgt gcgccagcag
    14161 ttcttccaga atcagacgac gttgcgccgg atgctgcccg gtttccagat cgctaagctg
    14221 tagcgtcggt ggcgggcggt gcaaagtgcg caacgcttcc ggtagcgtca tcattccttg
    14281 tgacagttcc ggcggcagga gttcttcaat ggcgcaggtg tcgagcagat ccagcgcctg
    14341 gtcggttaat ttacgcagcg tggcctgctt tacgccttcc gttgttggat aaaccggcgt
    14401 gagcgtttcc tgtaattctg gcgtgctgag atcgccctgc acgcggtatt ccgggtggat
    14461 catctccgca ccatatttac cgcgctttgc ttcgccataa gccagtacac ggcggcccgt
    14521 cgccaggcta tttttcattg ccgcgctgaa attgaaaaag cgcatggtga ggatgccgga
    14581 accgtcgctg atctggcagg tcatcatccg ccgaccgccg aaggagatat tgcagttcag
    14641 cacttcgcct tccaccgtgg cataaacgcc cggcagtagt tctccgatgg ggtagagatg
    14701 ggtgcgatct tcgtagcgca ggggaaggtg taagagtaga tcctgcacgg tatgcaggtt
    14761 gattttcgcc agtttgttac taagtgctgc gccaacgccc gttagggaac tgagtgggac
    14821 agcatctaac aggcgacctt tcatggcact taccctgcag cctgcatagt agcccaccag
    14881 tcggcatcag cttcgatctc gccttgctga ttgacgtggg gataaggcag gcctttgcgt
    14941 tttgcgactt tcgccagcac cggatagccg ccttcaaaca acaggcgttg ttgctctgct
    15001 tccggcaaca tgctgttttc acgcaggtac atgcctgcat tttgccgctg acgctgggct
    15061 tcgtaaagaa tgagggctga ggcaacggaa acattcagcg actgcaccat gccgatcatc
    15121 ggaatgatga tgtcctgatc cgccagggcc aatgcttcct gcgtgatgcc cgttttctcc
    15181 tgtcccatca aaatgcaggt cgggcgagtg taatcaattt cgcggaaatc gacagcgtta
    15241 tcagaaagat gggttgccag aatctgcatg ccctggcctt tgagatgagc gacggcatcg
    15301 ccaatggtgc ggtgtgtttt cacctgtacc cagctgttac tacccgccgc tgccgaagcc
    15361 atggtgcgca tgcggctacc aggccagacg gcgtgaactt catgtacgcc aacggcatct
    15421 gcggtacgaa taatcgcaga aacgttatga ggtttgtgga cctgctccat gcagacggtc
    15481 agatcaggct gccgcctggc gagcatttcg cagatgcgtg cataacgtgt tgggttcata
    15541 aaacattaat ttcggtttcg ggtgacttta atcacgtctg gcatcacgcg gattttgcgc
    15601 atgatattcg ccagatgcac acggtcacga gcggtcagac gaataaaggc gctgtagacg
    15661 cgaccatctt tctcttccgt attcaaactt tgaatattcg aggtcgtggt gttaattgcc
    15721 gccgtcaggt ttgccagcgc accctgatga ttgaacatct ccaccttgat ttcggtgatg
    15781 aactcctgcg ccgtctcttt atcccattcc acagccataa acttctctgg ctctttctgg
    15841 tagccacgga tgttacggca ggattcatgg tggatcacca gacctttacc ggggctgacg
    15901 tgggcgataa tcgggtcgcc aggaatgggg cggcagcatt tcgcaaaggt gatcagcacg
    15961 ccatcagcgc ctttgatcgg cagatgcccg tggctttgag tcgccggtgg aatggaggcg
    16021 tccccatgtt gtagattttt cgcgaccacc acgctcattg cgttaccaag gccgatttct
    16081 gccagcagat cgtcaagcgt tgccagcttc atgcgatcca gctcgcgctg aatattttcc
    16141 tgcgggattt cattcagctt acggctacca cccaaagcat ggttgagcag acgacggccc
    16201 aggcttacag aatcatcacg cttgaggttt ttcagcaact gacgaatttt ggcgcgcgct
    16261 tttgagctaa cgacaaagtt cagccacgcg gcattcgggc gagcgcccgg tgcggtaatg
    16321 atttcaacgg tttgaccgct ggtaagcggc tgcgacagcg ggtaaggctg gcggtcaacg
    16381 cgtgcgccca cgcaggcatg accgatatcg gtatgcactg cataagcgaa gtcgacgggc
    16441 gttgcaccgg caggcagctc gacaattcgc ccttccggtg tgaaaacgta aatctcatcc
    16501 gggaagagat cggatttaac gctctcgata aattcaaacg aactaccggc gctctgttgc
    16561 agctccagca ggctttgcat ccagcgctgg gcgcggattt gtgcggtagt actggtttcg
    16621 ccgtgctctt tataagccca gtgcgcggca acacccatct ccgccatctg atccatatct
    16681 tcggtacgga tctggacctc aaccggcacg ccgtgcgggc caatcatcga ggtgtgcaac
    16741 gactgatagc cgttcgcttt aggaatggcg atatagtctt tcacgcgacc cggacgcggc
    16801 ttgtacaggc tatgcatttg cccgagcacg cgataacagg tgtcagaatc attgacgatc
    16861 acgcggaaag cgtagatgtc catgattgag tgaaaacgct gctctttgag caccattttg
    16921 cagtaaatcg aataaagatg cttttcgcga ccactgacgc ggcacggtat tcccgcttcc
    16981 tgcaaacgcc cttcgatttc agagaggatt ttttggatca tctctttacg gttgccgcgc
    17041 gcggctttca ccacttcttt aattacgcgg taacgattgg gatacagcgc ctcaaaaccc
    17101 agctcttcga gttcggtttt aatgtggtgg atacctaaac ggtgcgccag cgggctataa
    17161 atttcgagag tttcacgggc gatgcggcga cgtttgtccg ggcgaagtga gcccagcgtg
    17221 cgcatgttgt gggtacggtc ggcaagtttg atgagaatga cgcggatatc ctgcaccatc
    17281 gccataatca tcttgcgaaa gttttcggcc tgcgcctctt tcttatcgcg gaacttgagc
    17341 ttatcaagtt tcgacacccc ctctaccagc tcggcgacgc ttttaccaaa aagctgttcc
    17401 atgtcctggt aggtggcggg agtatcttca atcacgtcat gcagcagcgc cgccatcagc
    17461 gtttcatagt cgagtttcat ctcggccaga atgcaggcaa ccgctaccgg gtgcgtgata
    17521 tagggttcac cgcttgaacg tgtttgcccc tcgtgagcat cacgtgcaac gagatacgcc
    17581 tgccgcagac gcttgatttg gtcttccggc aggtaggttt gaatcagttg attcaggctt
    17641 tcaaacagat acaagggcga cccgctttgt gattaacgac gaccttcagc aatagcggta
    17701 acggcttgta attcagcggc ttcctgctct tgctgttcct ggcgttcgcg aacgtcgagg
    17761 atctggttgt tgatcagacc ttcttcgatt tcgcgcagcg cgattacagt ggttttatcg
    17821 ttttcttccg gtaccagcgg atcctttccg cctacctgca tctgacgagc gcgacgcgcg
    17881 gcgaccagta ccaggtcaaa acggttacca attttctcta cagcgtcctg aacagttacg
    17941 cgtgccatac ttaaaaagct ccacaggtga agaaatgact gggcatgata ctgaaatcag
    18001 gttcagtctg ccaacaattt gctgattaaa gcgtcatgac gctgcttttg gcggctcatg
    18061 cgcagacgtt cggcgcgaat aatggtcttc aaatcggtca acgcggtatc gaagtcatca
    18121 ttcacaatca gataatcata ttcggcgtaa tggctcattt ctgcaacagc ttgcgccata
    18181 cgctttgcaa tgacctcttc gctgtcctga ccgcgaccgc gtagacggcg gtccagttca
    18241 attttggacg gcggtaaaat aaagatactc cgcgcgtgcg gcattttctg gcgaatttgc
    18301 tgcgcgccct gccagtcgat atcgagaaaa acatcgacac cggtcgccag tacttgctca
    18361 atggcctcac gcgaagtgcc atagtaatta ccaaaaactt ctgcgtgttc gaggaacgca
    18421 tctctgctaa tcatttcttt aaattcatca tgattaacaa agaaataatg ttcaccgtgg
    18481 acttcaccag gacgcggttg gcgtgtggtg tgtgaaacag aaacctgggt gtcatacaac
    18541 ggttgggttt ttaataaagc ctgaatcagg ctggatttac ccgcgccact gggggcagaa
    18601 acaatataaa gcgtgccttg agccatgagt atctttcgta tgtgattagc gaaataaagc
    18661 ctacatacga gcttattata cacggcgcgg ccatgtgacg tagcccttgc tcacactttt
    18721 tgcgcaaagt tgttgcgttt tgcgtagctg tttccgcatt tgcctgtgat ttactgcaat
    18781 aaagacgatt ctcaggaagc gtcctcgcag actccgtttt tgccactcgg ctttgccttc
    18841 accacgttgt atatctgcca gcctgactac agcggaggtg aagatgaaag tatggatggc
    18901 gatattaata agtatcttgt gctggcaatc atctgtgtgg gcggtctgtc cggcctggtc
    18961 gccagccaga gcacaggaag atatttcccg cctgcaacag caaataaaac agtgggacga
    19021 tgactactgg aaggaaggaa aaagtgaggt ggaagacggt gtttacgatc agttaagcgc
    19081 ccgtcttacg cagtggcaac gctgctttgg gagcgagccc cgcgacgtga tgatgccgcc
    19141 tttaaatggt gcggttatgc atccggttgc ccatacgggt gtacgcaaaa tggtggataa
    19201 aaacgcatta agtctgtgga tgcgagaacg tagcgatctt tgggtgcagc caaaagttga
    19261 tggcgttgcg gtaaccctgg tttatcggga cgggaaactg aacaaagcaa tcagtcgcgg
    19321 taatggcctg aaaggcgagg actggacgca gaaagttagc ttaatttccg ctgtgccgca
    19381 aaccgttagc gggcctttag ccaacagtac gcttcagggg gaaatatttc tccagcgcga
    19441 ggggcatatc caacaacaaa tggggggaat aaatgcccgc gcaaaagttg ctggcctgat
    19501 gatgcgccag gacgatagcg acacgctgaa ttctctgggc gtttttgtct gggcatggcc
    19561 ggatggaccg cagttaatga ccgatcgttt aaaggagctg gctaccgcag gttttactct
    19621 aacgcagagg tatacccgtg cggttaaaaa tgctgatgag gttgcgcgcg tacgcaatga
    19681 gtggtggaaa gcgaaattac ccttcgtcac cgatggcgtg gttgtgcgag gggcgaaaga
    19741 acccgaatcc cgccattggc taccaggcca ggcagaatgg ctggtggcct ggaaatatca
    19801 acctgtagct caggttgccg aagtgaaggc gattcagttt gcggtgggta agagcggtaa
    19861 aatatcggtg gttgcgtcac tcgcacctgt catgctggat gataaaaaag tccagcgggt
    19921 gaatattggt tccgtcaggc gctggcagga gtgggatatt gcgcctggtg atcagatcct
    19981 cgtcagcctt gccggtcagg gtattcctcg cattgatgat gtggtctggc gcggtgcaga
    20041 acgtacaaaa ccgacaccac cagaaaaccg ctttaactcg ttgacctgct actttgcttc
    20101 tgatgtttgt caggaacagt tcatttcacg cttagtctgg ctggggtcaa aacaggttct
    20161 ggggctggat ggcattggtg aggccggctg gcgcgcgctg catcagactc atcgctttga
    20221 gcatatcttt tcctggcttt tattaacgcc agagcaatta cagaacacgc cggggatcgc
    20281 gaaaagtaaa agtgcgcagc tatggcatca gtttaatctg gctcgtaagc agccttttac
    20341 tcgctgggtg atggcaatgg gaataccgct aacccgggcg gcgcttaatg cccgtgatga
    20401 acggtcctgg tcgcaacttt tatttagcac ggagcagttc tggcagcagc tgccggggac
    20461 tggatcgggg cgcgccagac aggttattga atggaaggaa aatgcgcaaa tcaagaagct
    20521 gggcagttgg ctggctgccc agcagatcac aggttttgaa ccttagtgtc cttcgtggct
    20581 gtagtaaaag accggtaatc ccagcttaag gcgtagcgcc agtaagcggg caaaaaagcc
    20641 gaataccagg gtggagataa ttaccacatc atggttagaa acatagtgtt gcagcgcgat
    20701 gtacagcacg gcggaggcaa atgagacacc ggcatacaac tctttctgga ataccagtgg
    20761 gatacgttta cagaacatat cgcgcaaaac gccgccaaac acgccggtgg ttaccgccgc
    20821 gacaacggca ataattgggc cgtgccccat atccagtgca acctgtgcgc cgatgataga
    20881 aaagaccacc agtccaagcg catcgagcac cagaaacact ttgcgcaggt aaggcattac
    20941 aggagcaacg atagtggtga gtactgcggc ggtagcgacg ataatcacat attccgggtg
    21001 tttgacccag ccgagcggat agtggcccag cagaatatcg cgcactgacc ctccgccaat
    21061 tgcggtggcg gtcgcaataa taattacgcc aaatgtatcc atgcggcgtc gcccggccgc
    21121 cagagccccc gtcatggctt cggcagtaat gccaaccaaa tacaaaatgt gtaacagcat
    21181 ggttctctcc ctaaaaatct gtctggcagg gtagcgattt gtgcgtattg tcacgattga
    21241 gattttttaa ggtgagagga aattgataac ttgtagttat gtgaaatcgc tattttctgt
    21301 agcgtagatg cattcttctg acttcttgtc tgagaaaatg cataagaaaa aataggttaa
    21361 aattgagggg tggaaaagaa aggtgcaatg tttagccatt gtgttcaccg cacctggact
    21421 ttggcttacg catcgggtat ctcaatcaac aaaaagagtt tttagtttct gctatttctt
    21481 ctctgtaatt tttacactgt tttccaactg cttgatgctg acctgcgggg tcggcaactc
    21541 ctcaggcatg gttccgccaa gttcctgaat ggtttgcctc accttgctac ccacgtcaaa
    21601 gtgtgtggta ttagcttgct gttttgaatt cacgccatcc cgcttgagtt tttcttctgt
    21661 ttgggtagct cgaaagagat tagccgccag ttctgttgaa cccatatgat ccaggatctt
    21721 ctgactcttt ttcagcccct tccgctgatg gatggctttc tgatctaatc caccatacag
    21781 cccccggtaa ccatgattct gaaatatggc gaaatcgata gctgtcgcta ccccagcttg
    21841 ctgcgctgcc tcaaccaatt gtttgttatg ttctttcaat tcatttctca gaaaaagacg
    21901 cttttcgtct tcacgaagtt gcctgaaagc ttcatcgtca gccagctcct gccgtcgggt
    21961 ctggatagca aaataagttt gccctgccgc aataaccggt ttcgcagggt cgccgttttg
    22021 tactaccagg taacaggcat agcgggagag atgaacatct tcaagttctc gttgagcccc
    22081 tgaacctaat acaaccattt tggtggtttc aacgaaatgg tcagatgcag cttgattgct
    22141 ggcttcgcac gcttgagtcg cgcgggccag taccttttga aaattgcgcc agtcccgata
    22201 atccagtaac ggtgccaatt ctctagctga ccaaaattct tgcccttcag tgccataatg
    22261 ccgtatttct tcaaaaggtt gatgatgttc gttcattgcc cactccatgt gatatcgatt
    22321 gcaaagaaag taaatctgta actgtattta tatacagttg tgagtatctc acgttctcag
    22381 tgtgctgggc aaaaaaacat cgcaaaatct ggagcaggca cgcaaaacag aattgaagtt
    22441 gcggggaaga aaaatgccgc tctgtttgag tgggcggcat ttgtgaaggc tgaaaagtaa
    22501 gaattggcgt tacgagttat tcaatgttct gaatctgctc gcgcatctgc tcaatcaaca
    22561 ctttcagctc aatcgcggag tttgtcactt cggcattgat agacttcgac gcaagagtgt
    22621 tcgactcgcg gttgaattcc tgcatcataa agtccagacg acgaccaacg gcttcttttt
    22681 tcttcagaat gttgtaggtc tctttgacat gcgcttccag gcggtccagt tcttcggcaa
    22741 cgtcaattcg ttgtgccagc agaaccagct cctgctccag acggttgttt tccaactgaa
    22801 cctgagcatc ttccagcttc gcgaccagac gctcacgctg ccattgcagg atttccggca
    22861 tatgggcgcg gactttgacc acttcggcgg tgacgccttc cagacgctgc tcgatcaatg
    22921 ctttcagtgc ctgaccttcg gtttcgcgcg cgacaataaa gtcgtccagc gtaccatcca
    22981 gcgccgcgag aatttcagcg gcaatggcgt caagatcctg ctcctgggct gccatcacgc
    23041 ccggccagcg tagaatatca accgggttga tttccccttc gtcactctgc atttttaccc
    23101 agttcgcggc agttaccagc tgtttagcca gtttttcgtt gaggatcagc tcaccttgcg
    23161 cgctaacatc tggctcatag cgcagggtac attccacttt accgcgcgtc aggcgagaac
    23221 gaatacgctc gcgaacgaca ggttcaaggc tacggaactg ctccggcaga cgaaagtaag
    23281 tttccagata acgctggttt accgagcgca tttcccaggt cgcgctcccc cattcaccct
    23341 tgatttcacg ccgggcgtag gcggtcatac tgcggatcat agacgttcct gtttataaaa
    23401 ggagaggtgg aaggattata gccatcgatg ccttgtcagg ataggaataa ccgccggaag
    23461 tccgtataat gcgcagccac atttgtttca agccggagat ttcaatatgc gtccagcagg
    23521 ccgtagcaat aatcaggtgc gtcccgttac cctgactcgt aactatacaa aacatgcaga
    23581 aggctcggtg ctggtcgaat ttggcgatac caaagtgttg tgtaccgcct ctattgaaga
    23641 aggcgtgccg cgcttcctga aaggtcaggg ccagggctgg atcaccgcag agtacggcat
    23701 gctgccacgt tctacccaca cccgtaacgc tcgtgaagcg gcgaaaggta agcagggtgg
    23761 acgcacaatg gaaatccagc gtctgatcgc ccgtgctctt cgcgcggcag tagatttgaa
    23821 agcgctgggt gagttcacca ttacgctgga ctgcgacgtg cttcaggctg atggtggcac
    23881 gcgtaccgcg tcgattacgg gtgcctgcgt ggcgctggca gatgcgctac agaagctggt
    23941 ggaaaacggc aagctgaaaa ccaacccgat gaaagggatg gtagccgcag tttctgtcgg
    24001 tattgtaaac ggtgaagcaa tttgcgatct ggaatacgtt gaagactcag ccgcagagac
    24061 cgacatgaac gtagtgatga ccgaagacgg gcgcatcatt gaagtgcagg ggacggcaga
    24121 aggcgagccg ttcacccatg aagagctact caccttgttg gcgctggccc gagggggaat
    24181 cgaatccatt gtagcgacgc agaaggcggc gctggcaaac tgatttttaa ggcgactgat
    24241 gagtcgcctt ttttttgtct gtagaaaagt aagatgagga gcgaaggcat gaaaccatat
    24301 cagcgccagt ttattgaatt tgcgcttagc aagcaggtgt taaagtttgg cgagtttacg
    24361 ctgaaatccg ggcgcaaaag cccctatttc ttcaacgccg ggctgtttaa taccgggcgc
    24421 gacctggcac tgttaggccg tttttacgct gaagcattgg tggattctgg cattgagttc
    24481 gatctgctgt ttggccctgc ttacaaaggg atcccgattg cgaccactac cgccgtggcg
    24541 ctggcggagc atcatgacct tgacctgccg tactgcttta accgcaaaga ggcaaaagac
    24601 cacggtgaag gcggcaatct ggttggtagc gcgttacaag gacgcgtaat gctggtagat
    24661 gatgtgatca ccgccggaac ggcgattcgc gaatcgatgg agattattca ggctaatggc
    24721 gcgacgcttg ctggcgtatt gatttcgctt gatcgtcagg aacgggggcg cggcgagatt
    24781 tcggcgattc aggaagttga gcgtgattac aactgcaaag tgatctctat catcaccctg
    24841 aaagatctga ttgcctatct ggaagagaag ccggaaatgg cggaacatct ggcggcggtt
    24901 aaggcctatc gcgaagagtt tggcgtttaa agaaactcgc cggatgaaaa gtcatccggc
    24961 gtcatattac tgcaactgcg cggcaattag cggccagcgg gcgtcaaaat catccgtcgg
    25021 gcggtattta aattcgctgc ggacaaaacg tgacagcata ccttcacaga aggccaggat
    25081 ctggcttgcc agcagggttt catcggtggt gtaaccttca ccctcacgca ttctcttttc
    25141 acgcaatacc tggcgcagct gcgcttcaat acgctcgaac agctggttga tacgcccttg
    25201 caggcgatcc tgttcaaaca tcagcgcatg accagtgagg atgcgggtca ggccaggatt
    25261 acgctcacca aaaccgagaa gcagcaacac aatcagacgc aggcgcgctg tggtgtcttt
    25321 ctcatctttc agaatcaggt tgatgcgagt aatcaggcta tcttcgataa actcaatcag
    25381 gctatcgaac atgcgggtct tactggggaa gtggcgatac agtgccgctt cggaaacgcc
    25441 gacagaggcg gccagttttg ccgtcgtgat acgttggctt ccatcgctgg attccagcat
    25501 cagcgccaga gactgaagta tttcctcgcg acggttcctt ttcgcagttt gtttttctgc
    25561 catgttacaa aatacccctg aaaataagca cttgccaggc ggcacccacg ctatgaccgc
    25621 aaacgaaatg tttgcggcta tgttatgacg ttattcggat gcgtatgtgt tactgacgac
    25681 cagagtgacc aaagccgcct tcaccgcggt cggtggcgtc gaaatcttcc accagattaa
    25741 attcagcctg tactaccgga acaaaaatca tctgggcgat gcgttcgcca ggttgaatgg
    25801 tgaagctgtc ctgaccacgg ttccacacgg aaatcatcaa ctggccctga tagtcagaat
    25861 cgatcaatcc taccaggtta ccaagcacga taccgtgctt atgtcccaat ccggagcgcg
    25921 gcagcatcat tgccgccagt gaaggatcgg caatatgaat cgccagcccg gtcggaacca
    25981 gcgtagtgtc acccggagcc agttctacgg cgtcgtcgag acaggcacgc aggtcaagtc
    26041 cggcagagcc agaggtggca taagtcggga gcggaaattc cttcccaacg cgcgggtcca
    26101 gaatcttaac gtcgattttt ttcatcataa cgggtcacga tctcgtcgag taataattgg
    26161 ccaaggagct ctttgcgctc aagcggtaag actttatctc cgtcctgcca gaaaaggtgt
    26221 aatgcgttgt tgtcgctgtt aaatccttga gttggctggg aaacatcgtt cgcgcagatc
    26281 agatcaaggt ttttacggat acgtttttgc cgggcgtatt cttccacatt atttgtttcg
    26341 gcggcaaatc caacgacgta gggtcgatgg tcttttagtg cggcaacgcc tgcgacgata
    26401 tcggggtttt taaccatttt tattgttaat tcatcaccct gcgtggcctg ctttttgatt
    26461 ttctctgggg ccacggtagc tgcgcgataa tccgccacgg cggcgcagcc gataaaaata
    26521 ttttgctgct gtacagaagc attcacggcg gcttccattt ccagcgcggt catcacatca
    26581 acacgtttaa caaacggtgg cgtcggtagt gaaaccggac ctgataccag cgtgacgttc
    26641 gcgccacgac gggcagcggc ggcggcgata gcaaaaccca tcttgccgga gctgtgatta
    26701 gagatataac gcacgggatc gagcggttca cgcgtcgggc cggcggtaat cataatgttc
    26761 agatgtttca ggtcgttgac gggcgaaaaa tgcgctaccg ccatatccac aatggttaac
    26821 ggatcgagca ttcgcccagg accgatatca ccacaagcct gactgccact gtctggcccc
    26881 cagatgagca aaccacggga agcaagcacc tctaaattat gctgcgtggc agcggcacgg
    26941 tacatctgct ggttcatggc ggggagcacg gctacaggcg caggtgtagc cagacaaatc
    27001 gtcgatacca ggtcattcgc cattccggca gcaacacggg caatcaaatc tgccgtggca
    27061 ggggcgagaa tcactaaatc agcccattta cccagctcaa tatggcccat agcggcttcg
    27121 gctgccgggt ccagcagact gtcggaaacg ggataaccag aaaccgcctg caagctaagt
    27181 ggggtgataa aggcttttgc cgcttcggtc atggctacgc ggacgtcggc cccgcgatcg
    27241 cgcaaacgac gcaccagttc aggggtttta taggcagcaa taccgccgct aacgccgaga
    27301 acgatttttt taccggccag gctcatgatg atttttcctg ttgggtgaca ccagaagttg
    27361 gcgattttat cacaatactt ttgttgtcgt gccttcacct gagaatcact ttgcgaggcg
    27421 ctttccagga ttgaaaactg gccgtcgatt taacggaacg gctatgacag gatgcgagca
    27481 ccacaaagga ggtgaaggtg aaaaacaatt cacagctgtt gatgccgcgc gaaaaaatgc
    27541 tgaagtttgg tattagcgcc ttaacggatg tcgagctgct ggcgctattt ctgcgtaccg
    27601 gaacgcgcgg taaagatgta ttaaccctgg caaaagagat gctggagaat ttcggctctc
    27661 tttatggctt gttaacctct gaatatgaac aatttagtgg cgttcatgga attggcgtgg
    27721 cgaaatttgc ccagttaaag gggattgctg aactggcgcg gcgttactac aacgtgcgga
    27781 tgcgtgaaga aagcccttta ctcagcccgg agatgacgag ggaattttta caaagccagc
    27841 tcacgggtga ggagcgggag atctttatgg tgatctttct cgactcccaa caccgggtta
    27901 taacgcatag ccgtcttttt tccggcacgc taaaccatgt tgaagtccat cctcgggaaa
    27961 ttatccgcga agcgataaaa ataaacgcct cggcgctgat ccttgcacat aatcaccctt
    28021 cgggttgtgc tgaacccagt aaagcggata aactcattac tgaacggata ataaagagtt
    28081 gtcagttcat ggatttacgc gtgctcgacc atatcgtgat tgggcgtgga gagtatgttt
    28141 cttttgccga acgcggctgg atttaacccg ctatgcgcga tccttcggga tctttgtctg
    28201 ttcgggactt gagcacatcg ctgagtcagc gtatactacg ccacctttga gaatctcggg
    28261 tttggcattt gggcctggca atcgagagtt cacatagaac tgcgatgacc gggctgtaaa
    28321 gcctgacgag gcgccaatac cccatacgaa gctcgagcta atttgatttt tggagaatag
    28381 acatgtcccg agtctgccaa gttactggca agcgtccggt gaccggtaac aaccgttccc
    28441 acgcactgaa cgcgactaaa cgccgtttcc tgccgaacct gcactctcac cgtttctggg
    28501 ttgagagcga gaagcgtttt gtcaccctgc gcgtatctgc taaaggtatg cgtgtaatcg
    28561 ataaaaaagg catcgataca gttctggctg aactgcgtgc ccgtggcgaa aagtactaag
    28621 tacttagagg aaataaatca tggctaaagg tattcgtgag aaaatcaagc tggtttcttc
    28681 tgctggtact ggtcacttct ataccactac gaagaacaaa cgtactaagc cggaaaaact
    28741 ggaactgaaa aaattcgatc cagttgttcg ccagcacgtg atctacaaag aagcgaaaat
    28801 caaataattc tcgctttgat gtaacaaaaa acctcgctcc ggcggggttt tttgttatct
    28861 gcttgccccc atattgactg catctgttca ttcctggaga tgctatgcct gaattacccg
    28921 aagttgaaac cagccgccgc ggcatagaac cgcatctcgt tggtgcaacc attcttcatg
    28981 cggtggtgcg caacggacgc ttgcgctggc cggtttcaga agagatctac cgtttaagcg
    29041 accaaccagt gcttagcgtg cagcggcggg ctaaatatct gctgctggag ctgcctgagg
    29101 gctggattat cattcatttg gggatgtctg gcagcctgcg catccttcca gaagaacttc
    29161 cccctgaaaa gcatgaccat gtggatttgg tgatgagcaa cggcaaagtg ctgcgctaca
    29221 ccgatccgcg ccgctttggt gcctggctgt ggaccaaaga gctggaaggg cacaacgtgc
    29281 tggctcatct tggaccggag ccgcttagcg acgatttcaa tggtgagtat ctgcatcaga
    29341 agtgcgcgaa gaaaaaaacg gcgattaaac cgtggctgat ggataacaag ctggtggtag
    29401 gggtagggaa tatctatgcc agcgaatcac tgtttgcggc ggggatccat ccggatcggc
    29461 tggcgtcatc actgtcgctg gcagagtgtg aattgttagc tcgggtgatt aaagcggtgt
    29521 tgctgcgttc gattgagcag ggtggtacaa cgctgaaaga ttttctgcaa agtgatggta
    29581 aaccgggcta tttcgcacag gaattgcagg tttacgggcg aaaaggcgag ccgtgtcggg
    29641 tgtgcggtac gccgattgtg gcgactaaac atgcgcagcg ggcaacgttt tattgtcggc
    29701 agtgccagaa gtaattcatg cgcgccggat ggcataccat ccggcataaa cgctacgcta
    29761 acttcgccat cagcgcctga tggacattct ccggcaggaa atgggtgaca tcgccctgat
    29821 ggcgcgccac ctctttcacc aacgatgaag agataaacga ccactctttc gacggcatca
    29881 gaaacacact ttccagttcc ggcattaagt ggcgattcat atgcgccagc tgcatttcat
    29941 attcaaaatc cgccaccgca cgcaggccac gaatcagcac cgtagcgtgt tgattacggg
    30001 cgaagttcgc catcaaatca ctaaacccga ccacttccac gttccccaga tgcgcggttg
    30061 cctgctgtgc cagtgccaca cgctcttcca gggtaaacat cggtttttta ctggggctgg
    30121 cggcaatcgc cagaataacg tgatcgaaca tctgcgtggc gcgcgtcacg atatcgatat
    30181 gaccattggt aatgggatcg aaagtacccg gataaatcgc ccgtttttgc ataacaacct
    30241 caatgcgttt tcggtggcag gtaaggttcc agcagttgaa gcagacgctg tagcgcgccc
    30301 tggttttgat acagtacttc aacggcatga cggccataga aactacggta atcggcgtcg
    30361 gtgagtaaag aggacacctc ttttgcaagc gtagtggcat cggtaacggt aatcagcccg
    30421 cttgcctgct ccagccgcgc gcaaatgtct ttaaagttaa aagtatgcgg ccccatcaat
    30481 accggaatag cgtgtgcggc agcttccagc ggattatgcc caccacgttc aaccagtgaa
    30541 ccgccaacaa aggcgagatc ggcaatgccg tacagtaaca tcaactcgcc catcgtatcg
    30601 ccaactacaa cctgcgtgct ggtggagggg acttcccctg aagagcgtgt gatatagctt
    30661 agtccagcct ggcggacaag attaatcgca tccgggaagc gttctggatg acggggtacc
    30721 aggatgagca ataaattcgg gaattgctgt aacaatgcct gatgtgcggc gatcaccaca
    30781 ctctcttcgc cttcgtgagt gctggtggca atccataccg ggcggtgtgg tgcccactgg
    30841 cggcgcagcg tcacggcttt agcagccaac tgcggcgtta cagaaatatc gaatttcagg
    30901 ctaccggtaa cggtcacctg attatttttt gcgcccagcg ccacaaaacg tgcaccatct
    30961 tcttcatttt gcgcagcaat cagcgtaata cgacgcagca agcgacggac gaatttaccc
    31021 agtttggcat aacctgcggc cgagcgggca gagagtcgcg cgttagcgat caccagcgga
    31081 attttacgtt tgtgtagcgc cgcaatcagg ttaggccata gttcggtttc cataatcaac
    31141 accagtttgg ggtcgacttt attcaggaaa cggttgagcg catcgggcag gtcatacggc
    31201 agataaacgt gctgaacatc cttcccgaaa gccgattgta cgcgctccga accggttggc
    31261 gtcatggttg taacggtaat cggtaaatca ggataacgat ggcgcagcgc gcgcaccaac
    31321 gggatcgccg ccagagtttc accgacggag acggagtgca gcataatgcc gcctggtttt
    31381 agcggatggc ggtaaaaacc gtaacgttca ccccagcgtt ttcgataggc cggagcctta
    31441 cgtccgcgca cccagagccg tatccagatc agcggctgaa taaggtagag aagggcggtg
    31501 taaagcaatt cgagcatagt aaatagctga cttatggatg tgctggggat tctatgtatt
    31561 tagctgtggc tttaccatta cttttcccgt ttttgactta aatagcaatt tacccgccaa
    31621 ttcttgctga acatctatac tttttttgta tttttatgcg attcattgat ttctgctgta
    31681 cgtgcaggaa ggaatcgcag agcaacagtg aaagaatatg gttgctaatg attttatttg
    31741 attgatttct ttattgaaaa tcaacaatgt gcttaaaaat cattcgggtc ggattgccgc
    31801 gaaagaagtg atacactagc gcgtcaatat cagtgcggta tcagtattca ggtagctgtt
    31861 gagcctgggg cggtagcgtg ctttttctgc ttcaccttaa tcggataatc tcaacaaaaa
    31921 gagttacttg tggataagcc atttcgaaga attttgctca ttaagatgcg ttttcatggg
    31981 gatatgttat taactactcc cgtcattagt tcgctgaaaa aaaattaccc tgacgcaaaa
    32041 atcgatgtgc tgctttatca ggacaccatc ccgatcctgt ctgaaaatcc agagattaac
    32101 gcgctctacg gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc caactttttt
    32161 catctcatca aggtattacg tgccaataag tatgacctta tcgtcaatct taccgatcaa
    32221 tggatggttg ctatactggt tcgcttatta aatgcccgtg tgaaaatttc ccaggattat
    32281 catcatcggc agtctgcttt ttggcgtaaa agtttcaccc atttggtgcc gttgcagggt
    32341 ggaaatgtgg tggaaagtaa cttatccgtg ctgacaccat tgggacttga ttcgttggtg
    32401 aagcagacaa ccatgagtta cccgcctgca agctggaaac gtatgcgtcg cgaacttgat
    32461 cacgctggtg ttggacaaaa ttatgtggtt atccaaccta cggcgcggca aatcttcaaa
    32521 tgctgggaca acgccaagtt ttccgctgtg attgatgcct tacatgctcg tggttatgaa
    32581 gtcgttctga cgtccggccc agataaagac gatctggcct gcgtcaatga aattgcgcag
    32641 ggatgccaga cgccaccagt aacggcgctg gctggaaagg tgaccttccc ggaacttggt
    32701 gcgttaatcg atcatgcgca gctgtttatt ggcgttgatt ccgcaccggc gcatattgcc
    32761 gctgcagtta atacgccgct gatatcgctg tttggtgcga cagatcatat tttctggcgt
    32821 ccctggtcaa ataacatgat tcaattctgg gcgggagatt accgggaaat gccaacgcgc
    32881 gatcagcgtg accgaaatga gatgtatctt tcggttattc cggcggcaga tgtcattgct
    32941 gctgtcgata aattactgcc ctcctccacg acaggtacgt cgttatgatc gttgcttttt
    33001 gtttatataa atattttccc tttggcggtt tgcagcgtga ttttatgcgt attgctcaga
    33061 cagtcgccgc ccgaggtcat catgttcggg tttataccca gtcgtgggaa ggcgaatgcc
    33121 ctgatgtatt tgaactgatc aaagtgccgg ttaaatcgca taccaatcac gggcgcaatg
    33181 cggagtattt tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat aaagtcgttg
    33241 gattcaacaa aatgccgggg ctggacgttt attatgccgc tgatgtttgt tatgccgaga
    33301 aagtagcgca ggaaaaaggc tttttctatc gcctgacgtc acgttatcgc cattatgccg
    33361 cctttgagcg ggcaaccttc gaacagggca agccgacaca gctgctgatg ctgacagata
    33421 agcaaatcgc cgatttccag aaacattatc agactgaagc ggagcgtttt catattctgc
    33481 caccggggat ttatcctgat cgtaaatata gccagcagcc agccaatagc cgtgaaatct
    33541 tccgtaagaa gaatggaata accgaacaac aatatttatt gttgcaggtc ggttcagact
    33601 tcacgcgtaa aggtgtcgat cgttccattg aagcacttgc ttcgttaccg gattcgctgc
    33661 gccacaacac attgctatat gttgttgggc aggataaacc gcgaaaattt gaggcgctgg
    33721 cagaaaaacg cggcgtgcgc agtaatgttc actttttctc ggggcgcaat gatgtctcgg
    33781 aattaatggc ggcggcggat ttattactgc atcctgccta ccaggaagcg gcgggaattg
    33841 tgctgctgga agcgattact gcaggattac cggtactaac aacagccgtt tgtggctatg
    33901 cgcattatat tgtcgacgct aattgcggcg aggctattgc tgagccattc cgccaggaaa
    33961 cattgaatga gattttacgc aaagcgttaa cgcaatcttc attgcgccag gcctgggcgg
    34021 aaaatgcgcg acattatgct gatacacaag atttatacag tctgccagag aaagcggcgg
    34081 atatcataac gggtggtctg gatggttgaa cttaaagagc cgtttgccac gttatggcgc
    34141 ggcaaagatc cttttgagga agttaaaacc ttgcagggtg aggtatttcg tgaactggaa
    34201 actcgccgta ctctgcgctt tgaaatggcg ggcaaaagct attttctcaa atggcatcgc
    34261 ggcacgaccc tgaaagagat aatcaaaaat ttactctcat tgcggatgcc agtattaggc
    34321 gctgaccgcg aatggaatgc gattcatcga ctgcgggatg tcggcgttga tactatgtat
    34381 ggggtggcat ttggcgaaaa aggcatgaat ccgctgacca gaacttcatt tattattacc
    34441 gaagatctga caccaaccat aagtcttgaa gattacagtg ctgactgggc gactaaccct
    34501 ccagatgttc gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt gcgcgatatg
    34561 catgctgcgg gcattaacca ccgtgactgt tatatctgtc atttcctgct gcacttgcct
    34621 ttttccggta aggaagagga gttaaaaatt tcggtaattg acctgcaccg ggcgcagctt
    34681 cgcacgcgcg ttccacgtcg ttggcgggat aaagatctta ttgggcttta tttttcttcg
    34741 atgaatatcg gcctgactca gcgggatatc tggcggttta tgaaagtgta ttttgccgcc
    34801 ccgcttaaag acattctcaa gcaggaacaa ggactgctgt cgcaagcaga agcaaaagcc
    34861 acaaaaatca gggaaagaac gattcgaaaa tcgttgtaac ccgtaactta tttttgccaa
    34921 aattttggat acagaataaa tatgtctcaa ctcaatgata gtgacatcat cctttttgag
    34981 tataattttc attatcaaaa tataagatct aaaaatactc ttgatatagc atttggtatt
    35041 gacagaaatt ttctttttgg atgtggtgta gcca
//
LOCUS       DAZGAN020000044        33489 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGAN020000044 DAZGAN020000000
VERSION     DAZGAN020000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 33489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.044"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..96
                     /locus_tag="ACHKYO_RS11715"
                     /old_locus_tag="pgaptmp_002343"
     CDS             <1..96
                     /locus_tag="ACHKYO_RS11715"
                     /old_locus_tag="pgaptmp_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4429183.1"
                     /translation="FNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            complement(137..1495)
                     /gene="dgcT"
                     /locus_tag="ACHKYO_RS11720"
                     /old_locus_tag="pgaptmp_002344"
     CDS             complement(137..1495)
                     /gene="dgcT"
                     /locus_tag="ACHKYO_RS11720"
                     /old_locus_tag="pgaptmp_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU4429184.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            2082..4505
                     /gene="pgaA"
                     /locus_tag="ACHKYO_RS11725"
                     /old_locus_tag="pgaptmp_002345"
     CDS             2082..4505
                     /gene="pgaA"
                     /locus_tag="ACHKYO_RS11725"
                     /old_locus_tag="pgaptmp_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HGU4429185.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYNHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            4514..6532
                     /gene="pgaB"
                     /locus_tag="ACHKYO_RS11730"
                     /old_locus_tag="pgaptmp_002346"
     CDS             4514..6532
                     /gene="pgaB"
                     /locus_tag="ACHKYO_RS11730"
                     /old_locus_tag="pgaptmp_002346"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HGU4429186.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVYPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            6525..7850
                     /gene="pgaC"
                     /locus_tag="ACHKYO_RS11735"
                     /old_locus_tag="pgaptmp_002347"
     CDS             6525..7850
                     /gene="pgaC"
                     /locus_tag="ACHKYO_RS11735"
                     /old_locus_tag="pgaptmp_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HGU4429187.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLTTTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            7852..8265
                     /gene="pgaD"
                     /locus_tag="ACHKYO_RS11740"
                     /old_locus_tag="pgaptmp_002348"
     CDS             7852..8265
                     /gene="pgaD"
                     /locus_tag="ACHKYO_RS11740"
                     /old_locus_tag="pgaptmp_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HGU4429188.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(8315..9103)
                     /gene="phoH"
                     /locus_tag="ACHKYO_RS11745"
                     /old_locus_tag="pgaptmp_002349"
     CDS             complement(8315..9103)
                     /gene="phoH"
                     /locus_tag="ACHKYO_RS11745"
                     /old_locus_tag="pgaptmp_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HGU4429189.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(9722..10993)
                     /gene="efeB"
                     /locus_tag="ACHKYO_RS11750"
                     /old_locus_tag="pgaptmp_002350"
     CDS             complement(9722..10993)
                     /gene="efeB"
                     /locus_tag="ACHKYO_RS11750"
                     /old_locus_tag="pgaptmp_002350"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HGU4429190.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(10999..12126)
                     /gene="efeO"
                     /locus_tag="ACHKYO_RS11755"
                     /old_locus_tag="pgaptmp_002351"
     CDS             complement(10999..12126)
                     /gene="efeO"
                     /locus_tag="ACHKYO_RS11755"
                     /old_locus_tag="pgaptmp_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HGU4429191.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(12184..13014)
                     /gene="efeU"
                     /locus_tag="ACHKYO_RS11760"
                     /old_locus_tag="pgaptmp_002352"
     CDS             complement(12184..13014)
                     /gene="efeU"
                     /locus_tag="ACHKYO_RS11760"
                     /old_locus_tag="pgaptmp_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HGU4429192.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(13557..15065)
                     /gene="putP"
                     /locus_tag="ACHKYO_RS11765"
                     /old_locus_tag="pgaptmp_002353"
     CDS             complement(13557..15065)
                     /gene="putP"
                     /locus_tag="ACHKYO_RS11765"
                     /old_locus_tag="pgaptmp_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HGU4429193.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(15224..15433)
                     /locus_tag="ACHKYO_RS11770"
                     /old_locus_tag="pgaptmp_002354"
     CDS             complement(15224..15433)
                     /locus_tag="ACHKYO_RS11770"
                     /old_locus_tag="pgaptmp_002354"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429194.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            15488..19450
                     /gene="putA"
                     /locus_tag="ACHKYO_RS11775"
                     /old_locus_tag="pgaptmp_002355"
     CDS             15488..19450
                     /gene="putA"
                     /locus_tag="ACHKYO_RS11775"
                     /old_locus_tag="pgaptmp_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4429195.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLAHQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(19490..20128)
                     /gene="rutR"
                     /locus_tag="ACHKYO_RS11780"
                     /old_locus_tag="pgaptmp_002356"
     CDS             complement(19490..20128)
                     /gene="rutR"
                     /locus_tag="ACHKYO_RS11780"
                     /old_locus_tag="pgaptmp_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HGU4429196.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            20416..21507
                     /gene="rutA"
                     /locus_tag="ACHKYO_RS11785"
                     /old_locus_tag="pgaptmp_002357"
     CDS             20416..21507
                     /gene="rutA"
                     /locus_tag="ACHKYO_RS11785"
                     /old_locus_tag="pgaptmp_002357"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HGU4429197.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEETLSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            21507..22199
                     /gene="rutB"
                     /locus_tag="ACHKYO_RS11790"
                     /old_locus_tag="pgaptmp_002358"
     CDS             21507..22199
                     /gene="rutB"
                     /locus_tag="ACHKYO_RS11790"
                     /old_locus_tag="pgaptmp_002358"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HGU4429198.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            22211..22597
                     /gene="rutC"
                     /locus_tag="ACHKYO_RS11795"
                     /old_locus_tag="pgaptmp_002359"
     CDS             22211..22597
                     /gene="rutC"
                     /locus_tag="ACHKYO_RS11795"
                     /old_locus_tag="pgaptmp_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HGU4429199.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            22605..23405
                     /gene="rutD"
                     /locus_tag="ACHKYO_RS11800"
                     /old_locus_tag="pgaptmp_002360"
     CDS             22605..23405
                     /gene="rutD"
                     /locus_tag="ACHKYO_RS11800"
                     /old_locus_tag="pgaptmp_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HGU4429200.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            23415..24005
                     /gene="rutE"
                     /locus_tag="ACHKYO_RS11805"
                     /old_locus_tag="pgaptmp_002361"
     CDS             23415..24005
                     /gene="rutE"
                     /locus_tag="ACHKYO_RS11805"
                     /old_locus_tag="pgaptmp_002361"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HGU4429201.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            24016..24510
                     /gene="rutF"
                     /locus_tag="ACHKYO_RS11810"
                     /old_locus_tag="pgaptmp_002362"
     CDS             24016..24510
                     /gene="rutF"
                     /locus_tag="ACHKYO_RS11810"
                     /old_locus_tag="pgaptmp_002362"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HGU4429202.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            24531..25859
                     /gene="rutG"
                     /locus_tag="ACHKYO_RS11815"
                     /old_locus_tag="pgaptmp_002363"
     CDS             24531..25859
                     /gene="rutG"
                     /locus_tag="ACHKYO_RS11815"
                     /old_locus_tag="pgaptmp_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HGU4429203.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVSSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(25942..26115)
                     /gene="ymdF"
                     /locus_tag="ACHKYO_RS11820"
                     /old_locus_tag="pgaptmp_002364"
     CDS             complement(25942..26115)
                     /gene="ymdF"
                     /locus_tag="ACHKYO_RS11820"
                     /old_locus_tag="pgaptmp_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGU4429204.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            26884..26957
                     /locus_tag="ACHKYO_RS11825"
                     /old_locus_tag="pgaptmp_002365"
                     /pseudo
     CDS             26884..26957
                     /locus_tag="ACHKYO_RS11825"
                     /old_locus_tag="pgaptmp_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            complement(27046..>33489)
                     /locus_tag="ACHKYO_RS11830"
                     /old_locus_tag="pgaptmp_002366"
     CDS             complement(27046..>33489)
                     /locus_tag="ACHKYO_RS11830"
                     /old_locus_tag="pgaptmp_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HGU4429205.1"
                     /translation="TGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIAD
                     ADPMEQPAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELT
                     ERPRLPAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAQAPE
                     NAAGRQGEILEGEPVRRGLPSPDAQNATAPVREGLPAPESQRGVDMPQPESLPRMVRD
                     SLPELAQQAEARRQTGDRQTITDVPDTEVPVDKPSTHQQARGAKIEDFGEEIKGAAKH
                     RYAQLAEAMGKTLEDGEYATQPLSKLFPKPDYARLEKEGVDSDTLAMMALYRSEIPTR
                     TSRNMQKWISIVKSGREATAGMLEGKIPAAKLADMMDSKPGLRSMSDTWKLLRTLSAL
                     QIDKASGYRVRSGVYSFVGGKRYDPPQMMYSLRDSKGRDLFFSESRDELLKKAKAYFD
                     EQGSRERETPATSADDRITFDVYRHKASGDIFIGYGKNRQKLKGGFESARDAHDYVRT
                     HRDELVNQVKALREVSREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFRGVQFGNYV
                     ESPRRQADLNRAYDSLHDLADVLNVPTKALSLNGRLGLAFGARGKGKAAAHYEPGEVA
                     INLTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDYMTEAQRAGRVFKDGRYV
                     DAEYPVRQEVYDAFKGVMKAINSSDMLRRSERLDGVRSKPYWSTDVEMAARAFERYVQ
                     DKARMAGVENDYLVNIRKADDHGQPDTYAYPTNAELDGGIREAFDHLFRTLKTRETDK
                     GVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVSQVEAVARGVMSGIKDSDLKVRVVK
                     SQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHAMEH
                     VVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEKQPN
                     KFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDGVGA
                     SSQYSRSGKPDPFKVPEGEGERYRDDLARMMKSLRSGAMTANIGRTPPVLRHLGAPDL
                     PLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVMLLDAVDKN
                     GDPVVSAVHMKATGKRLEINKVASVYGTENGKKLKSMEMAGLTLYRKEKLSRDNPQYS
                     GLQLPKEERSYRGSVDKILYPEDIRKGPYYSRTSSLTPEEIIISRFVCQMQDKFRVLK
                     AVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLADYKIAQAD
                     LDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGI
                     VDDMLARRRELIREAGLEESGVVDAWQNAYRYYVPLKGQDVDGMVSLPRTGKGFTIGG
                     RESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTD
                     DRPDTMRAIAERVDPETGETRREVVERPVPMAMMADRYFTTKKNGKTYYIKLHDPRLM
                     RAMKSMGPETSNAFVRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGR
                     SDGKLKGLDNLSALAVAKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEFVEDGGKTG
                     WFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALRLSAYKH
                     ARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRTLGHLNG
                     EGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHVKER
                     NLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGVLG
                     AFSPVGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQ
                     LGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLKFWIDYISGGTGRFISKTTD
                     AAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGAERT
                     AFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKSVERDMKVA
                     VDRFNREYNKKVGVE"
BASE COUNT         8130 a         8914 c         8182 g         8263 t
ORIGIN      
        1 tttaattata tcgaatgtga ttacaatcgg tggcggcggc acagttggtg tggcggcctc
       61 agtccggaac aatttgaaaa caagaacctc gcttaggcct gtgtccatat tacgtgggta
      121 ggatcaaaat gccgctttat ggtgactcac aaaaattcac cacatccctc acaaccacct
      181 tattacgccc ggtctctttc gcctcgtaaa gtgcgttatc agccagtcga tagatctcat
      241 tcgggtttaa cgcattttcc tgagtcacaa cagcgccaat actaatcgtc actttttgtg
      301 gtatagcgta ttcaggatta tcgccagtta aacgctcaac attttcccga atcctttccg
      361 ctaaagcttt cgcacgttca gtatcgatgt ccgtcagcaa gacgccaaac tcttcgccgc
      421 cgacgcgcgc taaaaggtca tctggtcgta tgcttttccc aatgatattc accactgttt
      481 ttatcacctg atcgccaacc ggatgccccc aggtgtcgtt gacttttttg aagtggtcga
      541 tatccataat catgacgcaa taaggcgttt ttttggctga tgctgattga actgtcagtt
      601 cattaaaaaa ataatttctg ttaaatatat tggttaaggg atcgcgatgt gcaatgttct
      661 ttgttactcg tagcgcactg aatatatggc acataaatat taccataacg gttaacttac
      721 tgactacttc aatagtgcga ctaatatacc aagtggacac gctataccta ctcataaata
      781 acagagagat attgtacagt acggcacaaa aagcaatcac cgtcactcca ttccagatat
      841 catagcgtaa accattaaag tatagattaa ccgaaagcaa ggttaaccac atgaaaatca
      901 ttatggtaac atatgaggct ttccaaacca cctgaccatt ttcattggtt aactcggcaa
      961 tgtgtaaatt gtagctctca taatgactag aaagtatgtg agcaactatc ggccccccaa
     1021 aaacaaaaaa caaacttatg cacaaagtca tttttttgga aaataaattg cgctgttttg
     1081 tgttttcact aactttattc accaatgcca gaaatattaa tatgcacaaa ctcatctgac
     1141 gaaacaaata atagattgca atgtcatttt ggacaacact gcttgtcaaa cgcccctcaa
     1201 taatttgctg aatgataatg acggttttaa caaagtaaat caaacaactc agaaaagcca
     1261 aactaagtat acagtttgaa aattgcctac ggtcataaat gaaatacaac tgcataaaaa
     1321 taaatgcaac agtatcgata aaaaataaag caatcattac catcagatag gttgatcgtg
     1381 gaacaacttc ctcaacgcct ggctgattaa accaactatt caccaaaacc aaagcaagcc
     1441 caaacaataa gctcactaat acagtactac taattctcaa atagtctttt tccataaagc
     1501 tacaccaatc tgtaggttgt agatcccttt ctattattaa tagatataag aactgtactg
     1561 ttgtcaatat gaaattccgc ccacaaacaa taccgatgat gatagttgct gaatgtaatt
     1621 attattgcct aagtgcttta tacattgttt gcaacaagcc aggcttgaaa atacttataa
     1681 atggttatag aaaatcctta taaaacatta attcatagat acattaggaa taacaattaa
     1741 atccgtgagt gccgtagcgc agcctttcat caggactttc gaaaaatccg aaatcatgca
     1801 tcggaattta ctgatttaat tattttaatc ctaatttatt ttgaaaaagg cattgggatt
     1861 tatgccgtat tcctgaagat cctcatcatt ggaatggatt ttcgggcgag aaaaggattt
     1921 tatatggaca ctctgctcat catttcttct tctcatcatc aacaattcac gtctctcttc
     1981 cgcgtttaat aacggattat gaggtgcaaa aatatctttc ttttcagtta cctgtaatta
     2041 gatacagaga gagattttgg caatacatgg agtaatacag gatgtattca agtagcagaa
     2101 aaaggtgccc gaaaaccaaa tgggctttga aacttcttac tgccgcattt ttagcagcga
     2161 gtcccgcggc gaagagtgct gttaataacg cctatgatgc attgattatt gaagctcgca
     2221 agggtaatac tcagccagct ttgtcatggt ttgcactaaa atcagcactc agcaataacc
     2281 aaattgctga ctggttacag attgccttat gggccgggca agataaacag gttattaccg
     2341 tttacaaccg ctaccgtcat cagcaattac cagcgcgtgg ttatgcagct gtcgccgtcg
     2401 cttatcgtaa cctgcaacaa tggcaaaact cgcttacact gtggcaaaag gcgctctctc
     2461 tggagccgca aaataaggat tatcaacggg gacaaatttt aaccctggca gatgctggtc
     2521 actatgatac tgcgctggtt aaacttaagc agcttaactc tggagcaccg gacaaagcca
     2581 atttactcgc agaagcctat atctataaac tggcggggcg tcatcaggat gaattacggg
     2641 cgatgacaga gtcattacct gaaaatgcat ctacgcaaca atatcccaca gaatacgtgc
     2701 aggcattacg taataatcaa cttgctgccg cgattgacga tgccaattta acgccagata
     2761 ttcgcgctga tattcatgcc gaactggtca gactgtcgtt tatgcctacg cgcagtgaaa
     2821 gtgaacgtta tgccattgcc gatcgcgccc tcgcccaata cgctgcatta gaaattctgt
     2881 ggcacgataa cccagaccgt actgcccagt accagcgtat tcaggttgat catcttggcg
     2941 cgttattaac tcgcgatcgt tataaagacg ttatttctca ctatcagcga ttaaaaaaga
     3001 cggggcaaat tattccgccc tgggggcaat attgggttgc atcggcttat ctcaaagatc
     3061 atcagccgaa aaaagcacag tcaataatga ccgagctctt ttatcacaag gagaccattg
     3121 ccccggattt atccgatgaa gaacttgcgg atctctttta caaccacctg gagagtgaaa
     3181 attatccggg cgcgctaact gtcacccaac ataccattaa tacttcgccg cctttccttc
     3241 ggttaatggg cacgcctacg agcatcccga atgatacctg gttacagggg cattcgtttc
     3301 tctcaaccgt agcaaaatat agtaatgatc ttcctcaggc tgaaatgaca gccagagagc
     3361 ttgcttataa cgcaccagga aatcagggac tgcgcattga ttacgcgagt gtgttacaag
     3421 cccgcggttg gcctcgtgca gcagaaaatg aattaaaaaa agcagaagtg atcgagccac
     3481 gtaatattaa tctggaggtt gaacaagcct ggacagcatt aacgttacaa gaatggcagc
     3541 aggcagctgt cttaacgcac gatgttgtcg aacgtgaacc gcaagatccc ggcgttgtac
     3601 gattaaaacg tgcggttgat gtacataatc ttgcagagct tcgtatcgct ggctcaacag
     3661 gaattgatgc cgaaggcccg gatagtggta aacatgatgt cgacttaacc accatcgttt
     3721 attcaccacc gctgaaggat aactggcgcg gttttgctgg attcggttat gccgatggac
     3781 aatttagcga aggaaaaggg attgttcgcg actggcttgc gggtgttgag tggcggtcac
     3841 gtaatatctg gctcgaggca gagtacgctg aacgcgtttt caatcatgag cataaacccg
     3901 gcgcgcgcct gtctggctgg tatgatttta atgataactg gcgtattggt tcgcaactgg
     3961 aacgcctctc tcaccgcgtt ccattacggg caatgaaaaa tggtgttaca ggcaacagtg
     4021 ctcaggctta tgttcgctgg tatcaaaatg agcggcgtaa gtacggtgtc tcctgggctt
     4081 tcactgattt ttccgacagt aaccagcgtc atgaagtctc acttgagggt caggaacgca
     4141 tctggtcttc accatatttg attgtcgatt tcctacccag tctgtattac gaacaaaata
     4201 cagaacacga taccccatac tacaacccta taaaaacgtt cgatattgtt ccggcatttg
     4261 aggcaagcca tttgttatgg cgaagctatg aaaatagctg ggagcaaata ttcagcgcag
     4321 gtgttggtgc ctcctggcaa aaacattatg gcacggatgt cgtcacccaa ctcggctacg
     4381 ggcaacgcat tagctggaat gacgtgattg atgctggcgc aacgctacgc tgggaaaaac
     4441 gaccttatga cggtgacaga gaacacaact tatacgttga attcgatatg acattcagat
     4501 tttaaggata aatatgttac gtaatggaaa taaatatctc ctgatgctgg tgagtataat
     4561 tatgctcacc gcgtgcatta gccagtcaag aacatcattt ataccgccac aggatcgcga
     4621 atctttactc gccgagcaac cgtggccgca taatggtttt gtagcgattt catggcataa
     4681 cgttgaagac gaagctgccg accagcgttt tatgtcagtg cggacatcag cactgcgtga
     4741 acaatttgcc tggctgcgcg agaacggtta tcaaccggtc agtattgctc aaattcgtga
     4801 agcacatcga ggaggaaaac cgctaccgga aaaagctgta gtgctgactt ttgatgacgg
     4861 ctaccagagt ttttataccc gcgtctaccc aattcttcag gccttccagt ggcctgctgt
     4921 atgggccccc gtcggcagtt gggtcgatac gccagcggat aaacaagtaa aatttggcga
     4981 tgagttggtc gatcgagaat attttgccac gtggcaacaa gtgcgagaag ttgcgcgttc
     5041 ccggctcgtt gagctcgctt ctcatacatg gaattctcac tacggtattc aggctaatgc
     5101 caccggcagc ttattgcctg tatatgtaaa tcgtgcatat tttactgacc acgcacggta
     5161 tgaaaccgca gcagaatacc gggaaagaat tcgtctggat gctgtaaaaa tgacggaata
     5221 cctgcgtaca aaggttgagg taaatccaca cgtttttgtt tggccttatg gcgaagcgaa
     5281 tggcatagcg atagaggaat taaaaaaact cggttatgac atgttcttca cccttgaatc
     5341 aggtttggca aatgcgtcgc aattggattc cattccgcgg gtattaatcg ccaataatcc
     5401 ctcattaaaa gagtttgccc agcaaattat taccgtacag gaaaaatcac cacaacggat
     5461 aatgcatatc gatcttgatt acgtttatga cgaaaacctc cagcaaatgg atcgcaatat
     5521 tgatgtgcta attcagcggg tgaaagatat gcaaatatca accgtgtatt tgcaggcatt
     5581 tgctgatccc gatggtgatg ggctggtcaa agaggtctgg tttccaaatc gtttgctacc
     5641 aatgaaagca gatattttta gtcgggttgc ctggcaatta cgtacccgct caggtgtaaa
     5701 catctatgcg tggatgccgg tattaagctg ggatttagat cccacattaa cgcgagtaaa
     5761 atacttacca acaggggaga aaaaagcaca aattcatcct gaacaatatc accgtctctc
     5821 tcctttcgat gacagagtca gagcacaagt tggcatgtta tatgaagatc ttgccggaca
     5881 tgctgctttt gatggcatat tgttccacga tgatgctttg ctttcagatt atgaagatgc
     5941 cagtgcaccg gctatcacgg cttatcagca agcaggcttt agcgggagtc tgagcgaaat
     6001 tcgacaaaac ccggagcaat ttaaacagtg ggcccgcttt aaaagtcgtg cgttaactga
     6061 cttcacttta gaacttagtg cgcgcgtaaa agccattcgc ggtccacata ttaaaactgc
     6121 acgaaatatt tttgcacttc cggtaataca acctgaaagt gaagcctggt ttgcacagaa
     6181 ttatgctgat ttcctaaaaa gctatgactg gaccgctatt atggctatgc cttatctgga
     6241 aggtgtcgca gaaaaatcgg ctgaccaatg gttaatacaa ttgaccaatc aaattaaaaa
     6301 catccctcag gctaaagaca aatctatttt agaattacag gcacaaaact ggcagaaaaa
     6361 tggtcagcat caggctattt cttcgcaaca actcgctcac tggatgagcc tattacaact
     6421 gaatggagtg aaaaactatg gttattatcc cgacaatttt ctgcataacc aacctgaaat
     6481 agaccttatt cgtcctgagt tttcaacagc ctggtatccg aaaaatgatt aatcgcatcg
     6541 tatcgttttt tatattatgt ctggtgttat gcatacccct atgcgtagcg tactttcact
     6601 ctggtgaact gatgatgagg ttcgttttct tctggccgtt ttttatgtcc attatgtgga
     6661 ttgttggcgg cgtctatttc tgggtctatc gtgaacgcca ctggccgtgg ggagaaaacg
     6721 caccagctcc ccagttgaaa gataatccgt ctatctccat tatcattccc tgttttaatg
     6781 aggagaaaaa cgttgaggaa accatacacg ccgctttagc acagcgttat gagaacattg
     6841 aagttattgc cgtaaatgac ggttcaacag ataaaacccg tgccatcctg gatcgcatgg
     6901 ctgcacaaat tccccatttg cgggtcattc atctggcgca aaaccagggg aaagccattg
     6961 cgcttaaaac cggagctgcc gcggcgaaaa gtgaatatct ggtgtgcatt gatggcgatg
     7021 cgttattaga ccgcgatgcg gcggcatata ttgtggaacc gatgttgtac aacccgcgtg
     7081 tgggtgccgt aaccggtaat cctcgtattc gaacacgttc taccctggtg ggtaaaattc
     7141 aggttggcga gtattcctca attattggtt tgatcaagcg aacccagcgt atctatggaa
     7201 acgtatttac cgtttccggt gttattgccg catttcgtcg cagcgccctg gcagaagtgg
     7261 gttactggag tgacgatatg atcaccgaag atattgatat tagctggaag ctgcagttga
     7321 atcagtggac gattttttac gagccacggg cgctgtgctg gatattaatg cctgaaacgt
     7381 taaaagggct gtggaaacag cgcctgcgct gggctcaggg cggtgcagaa gtattcctca
     7441 aaaatatgac aaggttgtgg cgcaaagaaa actttcgaat gtggccgctg ttttttgaat
     7501 actgcctgac gacaatatgg gccttcacct gcctggtcgg tttcattatt tacgcagtcc
     7561 aacttgccgg tgtaccgtta aatattgaat tgacacatat cgctgcgaca catactgccg
     7621 gaatattatt gtgtacgtta tgtttactgc aatttattgt cagcctgatg atcgagaatc
     7681 gctatgagca taatctgact tcatcgcttt tctggattat ttggttcccg gttattttct
     7741 ggatgctgag cctgacaacg acattggtat catttacacg agtcatgttg atgcctaaaa
     7801 agcaacgcgc ccgttgggta agtcccgatc gcgggattct gagaggttaa tatgaacaat
     7861 ttaattatta cgacccgaca atcaccagta cgtttactgg ttgattatgt tgccacaacc
     7921 atcttgtgga cattatttgc gttgttcata ttcttattcg ccatggatct gctgacgggt
     7981 tattactggc aaagcgaggc cagaagccga cttcagttct attttttgct ggcagtggcg
     8041 aatgccgtcg tgttaattgt ctgggcgctg tacaataagc tgcgttttca aaaacagcag
     8101 catcatgcag cctaccaata tacgccgcaa gaatatgcag agagcttagc aatacctgat
     8161 gagctctatc agcaactaca aaaaagccac aggatgagcg tacacttcac cagccagggg
     8221 caaataaaaa tggttgtttc agaaaaagcg ctagtccggg cataaacacc caaaacaaag
     8281 cccggttcgc ccgggctctg caccgataat acacttaact gtatgcatgc agcgtacgtt
     8341 ggcaaagtgc cgaacgtacg cagtcctctt taccgaaccg gacgatccca accatttcat
     8401 cttcttcgaa acgttccagc gcgtcactta atccggagca cacgccgcga ggcaaatcgc
     8461 attgcgtgat atcaccgttg acgataaccg tcacgttctc cccgaggcgg gttaaaaaca
     8521 ttttcatttg cgcggcagtc acattctgcg cctcgtcaag aatgacgact gcattttcaa
     8581 aggtacgtcc acgcatatag gcgaacggcg caatttccac cttccctatt tccggtcgca
     8641 ggcagtactg cataaaggaa gcccctaagc gccggaccag cacgtcgtag accgggcgaa
     8701 aatagggagc aaacttttct gcgatatctc caggtaagaa gccaagatct tcatcggctt
     8761 gcagaactgg acgggtgacg ataatcctgt cgacatcctt atgtatcagg gcctctgccg
     8821 cttttgctgc gctgatccag gtttttccgc acccggcttc gcccgtggcg aatatcagct
     8881 gcttactctc aatagccttc agatagtgca attgcgcttc atttcgcgcg aggatgggcg
     8941 aagtatcgcg actgtcgcgg gccataccaa tggcttctac gccgcccatc tgcacaagcg
     9001 aggtgaccga ttcttcttca cgctgcttat ggctgcgcga atcccgtctc agcacacgtt
     9061 ttgcctcacg acgagctttg atcactgctt tttgtcttcc catggagagc accttgagtt
     9121 gtttgtattc atcacacgcg ccgttggcag cgcgattatg cgcacgaaca tcagagggtt
     9181 ggcttccttg taagccatag tttgcttttg gataaaatgc cgaaaaacgg ctacgcacac
     9241 cgtttgcggc gtcggtaaca cgagtaaaga gaggaagtga gaaaaataca gagtgacgaa
     9301 gttgctaccg gaggagaaat ctccgcaagt ggtggagcgt agcttaacaa gacattgtcc
     9361 agaacaggac gttaccatcc gcgatctcca tagtgactga ctatcactgc cgggaacttc
     9421 cgctgctact taataattac aacagatctc gcattcattg caacaatata ttcacttata
     9481 tttaaccata aaacaccatt tcagtgacat tagtttctac tggaaagatg acagagtgat
     9541 gacagatatg aaaaaagctg tgtgctttca gcaggattcg catcaggcat ctgcgcacga
     9601 acgccggatg cggcgtgaac gccttatccg cctaccgaac ccgcaccgat tgtaggcata
     9661 agaagacgcg caagcatcgc atcaggcatc tgcgcaagaa cgccttatcc gtctataaac
     9721 attaaacccg caataacgcg cttccgagat aatcgttcgc gtccttcact cccggcagcg
     9781 caaaaaaata accgccgccg ataggtttaa cgtattcctc cagcgcttcg ccattgagcc
     9841 ttttttgtac agtcaggaag cctttttcca gatcgtgttg gtaacagaca aacagtagcc
     9901 ccatatccag ttgcccggag ttggtgacgc ccagtgaata actgtagcca cgacgcagca
     9961 tcaggctgga ctcactctcc gccgtgcggg gattcgccag ccggatatgg ctgtccagcg
    10021 caatcacctt cccttccgga tcgctggcgt aatcgggaac atcatgctca tgctgcattc
    10081 ccagcggcgc accggtttgc ttatcacgac caaaaatcgt ctgctgttct ttcagcggag
    10141 ttctgtccca aaattccact cgaaactgaa tcaagcgtac tgcctgatag ctgccaccga
    10201 ttgtccacgc tggctcctgc tgatctgccg ttacccacac cactttttgc atcaacttat
    10261 cattctggct atcgggattg gcagtgccgt ctttgaaacc cagcaaatta atcggcgtct
    10321 ctttgccttt actacgcgcc gcgtgatcgg aaataaaccc ttcccgcttc cagcgcacgc
    10381 tgagcaaatc tggcgtatgt ttgatgatat cgcgcagcgc atggatcacc gtgtcctggg
    10441 tgttggcgca aatttgtagc aacacatcac catgacataa cgccgcatcc agcgagtcgt
    10501 tgggaaaacg cgtcatcttc tgcaactttt tcggcatctg tggcgcaagg ccaaagcgct
    10561 catcaaacaa tgagtgaccc accgataacg tgatggtgag attatcgggc gcaatgtagc
    10621 cgccaagaat gccggaatcg agtggtggca ggcgcggatt tggcgtttct ggtgccgctc
    10681 cgccctgagt cagaaaagca aaacgctgag tcaacaagcg aaacaaccgt tcaagatcgg
    10741 gtttatcgct ggcaagcaca tcaaacgcca ccagcatcat tgcggcctgt tgtggcgtca
    10801 ggatccctgc ctgatgctca ccataaaacg gctgtttctc attgcgtgca tccggtgaaa
    10861 gcgtacccgg cgcactttgc gttttttgtg catgagcgac cggacaactt cccgccagcg
    10921 ccagcgcacc aatgcctttc agtaaacgtc ggcgtgacgg ttcattcacg ccgtttttat
    10981 cttcatactg cataatgctt aatccaatcc cagcacaccg cgaagttgcg ccagatcttc
    11041 cgccagcgca gtaatcggtc ctttcagtgc attccggtcg gcatcggtca atttgtcgta
    11101 ggtttcaaaa ccgtctttag tacggtattt cgccagaatg gtatcgacct ttttaaagtt
    11161 ggcatcgact tttgccaaca gttccgggtt ggctttttgc agttgtggac gcagcagatc
    11221 gacaattttc tgcgagcctt caacgttagc ctggaaatcc cacagatcgg tgtggctgta
    11281 gcgatcttct tcaccgctga ttttgctggc tgccacttcc tcaatcagtc cggctgcacc
    11341 gccgaccact tttgaaggtg ggaaagccag ttcactgatg cgtttttgca aatcgaccac
    11401 atcggtataa agctggtcag cgtactgatc catgcctttg gtggtgttgt cgccaaacaa
    11461 tgctttttcc agacggtgga aaccggtgaa tttcggatcg gcggcttttt gctcgtaatc
    11521 atcttcacgg gcgtcaatgc tgccatccag atcggagaac agttcagcaa tcggttcgat
    11581 gcgctcatag tgctggcgcg tcggcgcata cagtgctttc gctttttcga tatcgcctgc
    11641 tttaatagcg tcagtaaagg ctttcgtgtc ggtcaccagc tgcgtggttt ccgccatgac
    11701 atacgcttta tatgcagtaa ttgcaccacc aagacttaac agcgcatcac tttgcgccgc
    11761 atccgccgtt gcctcacctt tgacgatcaa cttccctttc gggttagtca gcagaccgca
    11821 ggtcatatcg tattcgccag gctgtaaatt cgccgtcatt ttctggctaa agccaggggc
    11881 gatattttcc cgctcttcca ccaccatcac gcctttgagg atctcccact ccagcgcctt
    11941 ctggctgtgg ttctgaataa tgaactgtgt tttcccggcg ttaaccgtaa tggtcatcgg
    12001 ttcgcactgc ttatcggtca cggtcacctt gacctgcggc acattagcgg cgttagccat
    12061 aaaagcagaa gagaacagcg cagccacgct caactgcaat gcgttacggc ggaagttaat
    12121 ggtcatgacg agactatccc tttaaagtat gttgtaacta agcaagagtt tgcgtcgtat
    12181 ttgctacgcg gagcgagacg ctgtcgcccc tgcgcgtggt ggcagggcaa atgccaccag
    12241 cgccgggatg agataaataa accagacggc gacttcgctg acgctcggtg cttcctgata
    12301 gccaaaaatc ccttccatca gcgtgccaaa cagcgagtga gttgagagga ccgcgctcat
    12361 atcgaaggcg atttcctgaa agtggttcca caatccggct tcatgaaatg cgcgaatggc
    12421 accagccgcc agccctgcgg cgacgaagag aataaacagg ctggtccatt taaaaaatgc
    12481 accaagattg aggcgaatac cgccccagta gagcaggaag ccgagcacca cggcagtagc
    12541 aagaccgagc attgcaccca gcggcggcca gatcccgaca tcttgttgaa atgccgccag
    12601 caggaaaaag accgactcca gcccttccct tgcaacggca aaaaagacca tcatcaccag
    12661 cgcccagcca tgatgatttc cacgctgcaa tgcgctatcg actgcctgtt ccagttgcac
    12721 tttgacgttg cgcgacactt tgcgcatcca gaaaaccatc caggtaagga tcaccacggc
    12781 gatcaccgcc acgatacctt caaacagttc ctgttctttt tgcggaaatt cgccggtggt
    12841 ttcgttaatg aagataccca ggcccaggca caacgcagcg gcaagcaaca cgccaatcca
    12901 catcacacca atccatcggc ctcgctgggt acgcttaaga tagctggcaa tcaaactgac
    12961 aatcagcgcg gcttcaagtc cttcgcgcaa cataatgaga aacggaacaa acatgccacc
    13021 cacccttaaa cgtttttctg agtcaatcta tgcaaagaaa agtaaatcgc agtgatagcg
    13081 attatcatta cggtaaagaa aaatacaagc ggaatgttta taaaatgatg ttggaatgta
    13141 aacaaagaga ggtaatgcca agggatataa acgattattc cagggtagaa agatgggatt
    13201 gtttgcaaag aaccatgtgt tcttgccgga tgccgcgtaa acgccgtatc cagccgacaa
    13261 aactgtccag actcaatgca tcagagcttc catgtaaaac tgataagcgg gtgtatcaag
    13321 caatttcgtc catccgcgat tatcaaatct aactttttgc ccggaacata ataacagctc
    13381 gggtgttggt ggcgtcaaaa tcgctgaagc gttccccgaa ggccgggaca gcagggggcg
    13441 gcggcgattg gccgccccct gcgcgctccc tgcaccattc aatttatatg gcgaggcaca
    13501 ttaagggagc gcaacccgct gctgaagcca aacaaaaccg ccgcaggcta agtcccttag
    13561 ctttcctgca accgtgacgg cggagccgaa tgatagtgcg catcggcctc ggcaaagcgt
    13621 ttttgcatcg ccgctgacgg cgctttaccc agcaaactaa acactacaat cccaatactg
    13681 ccgaagataa agcccggaat aatttcgtag agtcccagcc agccgaactg cttccagacg
    13741 ataaccgtca gcgcaccgat gatcatcccc gccagcgcac cgttgcgcgt catgcgtgac
    13801 cacatcaccg agaacagcac caccggacca aacgccgcgc caaagcctgc ccacgcgtag
    13861 ctcactaagc ccagcacgcg gttttccggg tttgccgcca gcgcaatcgc caccagcgcc
    13921 accaccagca ccatcacacg ccctacccac accagctctt tctggctggc atgtttacgc
    13981 agaaacgctt tgtataaatc ttcggtaatc gcactggagc acaccagcag ctggcagctt
    14041 aacgttgaca ttaccgccgc cagaattgcc gacagcagaa tcccggcaat ccacgggtta
    14101 aacagaattt gcgccagttc gataaacaca cgttcagcgt tctggtttac cgcacctgct
    14161 accgccgggt gctcattaaa gtaagcaatc ccaaagaagc cgacagccac cgcccctgcc
    14221 aggcagagga tcatccaggt catactgata cgacgcgcat ggacaatgct gtggtgagaa
    14281 tccgccgcca taaaacgcgc cagaatatga ggctgaccga agtaacccag cccccaaccc
    14341 atcaatgaga taatggcaac aaagttcagc cctttgagca tatccacgtt ttcgatgctc
    14401 ttttgtttga tcacttccag cgagtcacca aagccaccga cactgataat gacgataacc
    14461 ggcgtcagga tcagggcaaa aatcatcagg ctggcctgta cagtgtcagt ccagctcacc
    14521 gcgaggaaac cgccaataaa ggtgtaaagg atcgtcgccg cagcgcccgc ccacagagcc
    14581 gtttcgtagc tcatgccaaa ggtactttca aacagacgcg cgcctgccac aatgcccgaa
    14641 gcgcaataaa tggtgaagaa cagcaaaata accagcgcag agataatgcg caaaatgcgg
    14701 cttttatctt caaagcgccc ggtgaaataa tccggcaatg ttaaggcgtt attgttgtat
    14761 tcggtatgca cacgcaaccg cccggccacc agcttccagt tgatccacgc gcctaatgtc
    14821 aggccaatgg cgatccagct ttcggaaatc ccggaaagaa aaaccgcgcc cggcaacccc
    14881 attaacagcc agccgctcat atcagacgca cccgccgata atgccgtcac gaatggccca
    14941 aggctacgac cgcccagaat atagtcgtcg aagtttttcg ttgatcgcca ggcgataaac
    15001 ccaatcaata tcatgccaaa gatatagaca caaaatgtca ccaacatcgg tgtgctaata
    15061 gccatctaaa gtctccaaaa aattattatc ggcaatgtcg aaacttgccg ttatatctgc
    15121 caccggaacg gggtaacaga gtttatgttt taccagggcg accgtatcct gccggaagcg
    15181 ctggttattc acaatcgatt taacacacca tttacattaa attttagtgc tcagcgacac
    15241 tatttttcat caggttgcac tctctcacat tttttgcggt tgcacctttc aaaaatgtta
    15301 actgccgcag agaaaaagtc tgagttattt ttttaatccc tgtcatatcg atttctttta
    15361 ttaacatttc attcattttt aagcttgcta cgcatgtcac atttaacatg gttgcacaaa
    15421 gttgcaacat catggatatt tcacgataac gttaagttgc acctttcaga acaacaggag
    15481 taatggcatg ggaaccacca ccatgggggt taagctggac gacgcgacgc gtgagcgtat
    15541 taagtctgcc gcgacacgta tcgatcgcac accacactgg ttaattaagc aggcgatttt
    15601 ttcttatctc gaacaactgg aaaacagcga tactctgccg gagctacctg cgctgctttc
    15661 tggcgctgcc aatgagagcg atgaagcacc gactccggca gaggaaccac accaaccttt
    15721 cctcgacttt gccgagcaaa tattgcccca gtcggtttcc cgcgccgcga tcaccgcggc
    15781 ctatcgccgc ccggaaaccg aagcggtttc tatgctgttg gaacaagctc gcctgccgca
    15841 gccagttgct gaacaggcgc acaaactggc gtatcaactg gccgataaac tgcgtaatca
    15901 aaaaaatgcc agtggtcgcg caggtatggt ccaggggtta ttgcaggagt tttcgctgtc
    15961 atcgcaggaa ggcgtggcgc tgatgtgtct ggcagaagcg ttgttgcgta ttcccgacaa
    16021 agccacccgc gacgcgttaa ttcgcgacaa aatcagcaac ggtaactggc agtcacacat
    16081 tggtcgtagc ccgtccctgt ttgttaatgc cgctacctgg gggctgctgt ttactggcaa
    16141 actggtttcc acccataacg aagccagcct ctcccgctcg ctgaaccgca ttatcggtaa
    16201 aagcggtgaa ccgctgatcc gcaaaggtgt ggatatggcg atgcgcctga tgggcgagca
    16261 gttcgtcact ggcgaaacca tcgcggaagc gttagccaat gcccgcaagc tggaagagaa
    16321 aggtttccgt tactcttacg atatgctggg cgaagccgcg ctgaccgccg cagatgcaca
    16381 ggcgtatatg gtttcctatc agcaggcgat tcacgccatc ggtaaagcgt ctaacggtcg
    16441 tggcatctat gaaggtccgg gcatttcaat caaactgtca gcgctgcatc cgcgctacag
    16501 ccgcgcccag tatgaccggg taatggaaga gctttaccca cgtctgaaat cactcaccct
    16561 gctggcgcgt cagtatgata ttggtatcaa cattgacgcc gaagaggccg atcgtctgga
    16621 gatctccctc gatctgctgg aaaaactctg tttcgagcct gagctggcag gctggaacgg
    16681 tatcggtttt gttattcagg cttatcaaaa acgctgccca ctggtgattg attacctgat
    16741 tgatctcgcc acccgcagcc gtcgccgtct gatgattcgc ctggtgaaag gcgcgtactg
    16801 ggatagtgaa atcaagcgtg cgcagatgga cggccttgaa ggctatccgg tttatacccg
    16861 caaggtgtat accgacgttt catatctcgc ctgtgcgaaa aagctgctgg cggtgccgaa
    16921 tctaatctac ccgcagttcg cgacgcacaa cgcccatacg ctggcagcga tttatcaact
    16981 ggcaggtcag aactactatc cgggtcagta cgagttccag tgcctgcatg gtatgggcga
    17041 gccactgtat gagcaggtca ccgggaaagt tgccgacggc aaacttaacc gtccgtgtcg
    17101 tatttatgct ccggtcggta cccatgaaac gctgttggcg tatctggtgc gtcgcctgct
    17161 ggaaaacggt gctaacacct cgtttgttaa ccgtattgcc gacacctctt tgccactgga
    17221 tgaactggtc gccgatccgg tcactgctgt agaaaaactg gcgcaacagg aagggcaaac
    17281 tggattaccg catccgaaaa ttcccctgcc gcgcgatctt tacggtcacg ggcgcgacaa
    17341 ctcggcaggg ctggatctcg ctaacgaaca ccggctggcc tcgctctctt ctgccctgct
    17401 caatagtgca ctgcaaaaat ggcaggcctt gccaatgctg gaacaaccgg tagcggcagg
    17461 tgagatgtcg cccgttatta accctgcgga accgaaagat attgtgggct ttgtgcgtga
    17521 agccacgccg cgtgaggtag aacaggcgct ggaaagtgcg gttaataacg cgccaatctg
    17581 gtttgccact cctccggttg aacgcgcggc gattttgcat cgcgctgccg tgctaatgga
    17641 aagccagatg cagcaactga ttggtattct ggtgcgtgaa gccggaaaaa ccttcagtaa
    17701 cgccattgcc gaagtgcgcg aagcggtcga ttttctccac tactatgccg gacaggtgcg
    17761 ggatgatttc gctaacgaaa cccaccgtcc attagggcct gtggtgtgta tcagtccgtg
    17821 gaacttcccg ctggctattt tcaccgggca gattgctgcc gcactggcgg caggtaacag
    17881 cgtgctggca aaaccggcag aacaaacgcc gctgattgcc gcgcaaggga tcgccatttt
    17941 gctggaagcg ggtgtaccgc caggcgtggt gcagttgctg ccaggtcagg gggaaaccgt
    18001 gggcgcgcaa ctgacgggtg atgatcgcgt acgcggggtg atgtttaccg gttcaaccga
    18061 agtcgctacg ttactgcagc gcaatatcgc cagccgcctg gacgctcagg gtcgccctat
    18121 tccgctcatc gctgaaaccg gcggcatgaa cgcgatgatt gtcgattctt cagcactgac
    18181 cgaacaggtc gtcgtggatg tactggcctc ggcgttcgac agtgcgggtc agcgttgttc
    18241 ggcgctgcgc gtgctgtgcc tgcaagatga gattgccgac cacacgttga aaatgctgcg
    18301 cggcgcaatg gccgaatgcc ggatgggtaa tccgggtcgc ctgaccaccg atatcggtcc
    18361 ggtgattgat agcgaagcga aagccaatat tgagcgccat attcagacca tgcgtagcaa
    18421 aggccgtccg gtgttccagg cggtgcggga aaacagcgaa gatgcccgtg aatggcaaag
    18481 cggcaccttt gtcgccccga cgctgatcga actggatgac tttgccgaat tgcaaaaaga
    18541 ggtctttggt ccggtgctgc atgtggtgcg ttacaaccgt aaccagctac cagagctgat
    18601 cgagcagatt aacgcttccg gctatggtct gacgcttggt gtccatacgc gtattgatga
    18661 aaccatcgcc caggtcactg gctcggccca tgttggtaac ctgtatgtta accgtaatat
    18721 ggtgggcgca gtggtgggtg tgcagccatt cggcggcgaa gggttgtccg gtaccgggcc
    18781 gaaagcaggc ggtccgctct atctctaccg tctgctggcg aatcgcccgg aaagtgcgct
    18841 ggcagtgacg ctcgcgcatc aggatgcaga gtatccggtc gatgcacagt tgaaagccgc
    18901 attgactcag ccgctaaatg cactgcggga atgggcagca aatcgtccag aattgcaggc
    18961 gttatgtacg caatatggcg agctggcgca ggcaggaaca caacgattgc tgccggggcc
    19021 gacgggtgaa cgcaacacct ggacgctgct gccgcgtgag cgcgtgttgt gtattgccga
    19081 tgatgagcag gatgcgctga ctcagctcgc cgccgtgctg gcggtgggca gccaggtact
    19141 gtggccggat gacgcgctgc atcgtcagct agtaaaggca ttgccatcgg cagtcagcga
    19201 acgtattcaa ctggcgaaag cggaaaatat aaccgctcaa ccctttgatg cggtgatctt
    19261 ccacggtgat tcggatcagc ttcgcgcatt gtgtgaagcc gttgctgcgc gggatggcgc
    19321 aattgtttcg gtgcagggtt ttgcccgtgg cgaaagcaat atccttctgg aacggctgta
    19381 tatcgaacgt tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac
    19441 gatcggttaa acaggtagcc ggaggatgtt acaacctcct ccggcatctt taacgtggtc
    19501 gaatcccctc aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat
    19561 cgcgcaacgt cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag
    19621 tggaagccca aatcatgaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt
    19681 tgacccaacc ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca
    19741 gttcatccat taacagcggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct
    19801 gcggataatc gcgggagact tccagcttca gacggatgta ctcttttatc gccgccagcg
    19861 gagcgaaatc ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca
    19921 gcacggcaat atacagcgcc tcttttgacg gaaagtaata cagcagattg gtttttgaaa
    19981 cacccgccaa ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag
    20041 tgtccagtgc tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac
    20101 ccgttgtttt cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggcaacagga
    20161 tagcaaaacc cccgcccggc ctcgaccttc tgcactctca tcgcgcacag atgcatgttt
    20221 tatgtgcaac tgttttgacc gtttagtcca ctttttacca gatattttag acaacacaat
    20281 gttaacacat taataacatt cactttttaa aaactggcat ccgctttgca aacaagccat
    20341 tacgcggctg aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac
    20401 gaagagaggt tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc
    20461 atttcgaccc acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgtgcaa
    20521 aaagcggagc actaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc
    20581 ggcaaaactg agttctggga tcacaacctt gagtcgttca ccttgatggc ggggctggcg
    20641 gccgtcacct cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca
    20701 atcgtcgccc gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac
    20761 ctcgtgactg gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac
    20821 tatttctccc gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg
    20881 ggcacgggga aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt
    20941 ccgcaaccga gtgtccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg
    21001 ttctccgccc agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacgcccacg
    21061 gctttcgccc cgaccgctgc gcggatgaaa caggccgcag agcaaaccgg gcgcgacgtt
    21121 ggttcttatg tgttgtttat ggtgattgcc gatgaaaccg acgatgccgc tcgcgccaaa
    21181 tgggaacact acaaagcggg cgcggatgaa gagacgttaa gctggctaac cgaacaaagt
    21241 cagaaagata cccgctccgg tactgatacc aacgtccgtc agatggccga tcccacttcg
    21301 gcggtaaaca tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta
    21361 gatgaagtcg caagcgtgcc tggtgccgaa ggcgtgctgt tgaccttcga tgattttctg
    21421 tcgggaatcg aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatcta
    21481 cctgcgctga ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta
    21541 ccttcgatcc gcagcaaagt gcgctgatcg tggtggatat gcaaaacgct tatgccacgc
    21601 caggcggcta cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca
    21661 acattcaaac cgccgtgacc gcagcgcgag cggcagggat gctgatcatc tggtttcaaa
    21721 atggctggga tgaacagtat gtcgaagctg gcggccccgg ctcaccgaat tttcataaat
    21781 cgaacgccct gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag
    21841 gctcctggga ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc
    21901 cgaagccgcg ctacagcggt ttcttcaata caccgctgga cagcattttg cgcagccgtg
    21961 gaatacgcca tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac
    22021 gcgatggctt ttttctggag tatttcggcg tggtgctgga agacgcaaca caccaggcgg
    22081 ggccggaatt tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca
    22141 gcgacgtcga aacgttctgc gacgcgcttt ctcccacatc ctttgctcgt atcgcttaag
    22201 gagtttaacg atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc
    22261 cttcgttccc ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga
    22321 tcaacataat aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga
    22381 aattatccgc aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag
    22441 catctttatt accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt
    22501 tccgggtgat aaaccggcgc gattctgcat tcagtgcgga ctggtaaaac ctgacgcgct
    22561 ggtggaaatc gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct
    22621 cacctccccc ttatgctgat gcgcccgtag tggtgttgat ttcgggtctt ggcggtagcg
    22681 gcagttactg gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg
    22741 accagcgcgg caccggcaat aatcccgaca cgctggcaga agattacagt atcgcccaga
    22801 tggcagcgga actgcatcag gcgctggtag ccgcagggat tgagcattac gcggtggtcg
    22861 gccatgcgct cggtgcgctg gtgggaatgc agctggcgct ggattatccc gcgtcggtaa
    22921 ctgtgctgat cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc
    22981 aggttcgcga acgattactg tatagcggcg gcgcgcaggc atgggtggaa gcgcagccgt
    23041 tgttcctcta tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg
    23101 cgctggcact ggcgcatttt cagggcaaaa ataatttact gcgtcgactt aacgccctca
    23161 aacgcgctga ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg
    23221 ccagtgatga tctgctggtg ccatcagcat gttccagtga gcttcatgcc gccctgcccg
    23281 atagccagaa aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa
    23341 cgtttaatgc tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc
    23401 tgtaaggaat tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ttgttcaccg
    23461 atgcccgcac tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacgggaga
    23521 tttatgccct gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt
    23581 ttatccgcac ggcagaagga aaagaacgtc tgcgcccggc actttccagc ggcaatctgc
    23641 aaaaaaccct gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac
    23701 ggttaccgca actgtttcct cacggcgatg cccgcagttg gtttacctcc agcccacaac
    23761 ttgccgaaga aacagcgttt cgcaacagtt ccatgcaggc ggcctatctg atcgtcgcct
    23821 gccgtgcgct gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg
    23881 acgccttttt tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg
    23941 atagcagcaa actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt
    24001 tgtaaggagc aaaccatgaa cattgtcgat caacaaacct ttcgcgatgc gatgtcctgt
    24061 atgggcgcgg cagtcaatat catcaccacg gatggtccag ccgggcgcgc cgggttcacc
    24121 gccagcgcgg tctgcagcgt gaccgacacg ccgcctacat tactggtgtg cctgaatcgt
    24181 ggggcgtccg tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc
    24241 gccgggcagg agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt
    24301 gccgccgccc gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt
    24361 tcgtttgact gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc
    24421 gccatcgaag cgattcatcg tcacgccaca ccctacgggc tggtgtggtt cgatcgcagt
    24481 tatcacgcgc tgatgcgccc tgcttgttaa cctcctaaag gagacagctg atggcaatgt
    24541 tcggttttcc tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgt
    24601 atgaacgatt gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt
    24661 ttggcgcgac ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa
    24721 tgtcggggat cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc tccagttatc
    24781 tcggttccag cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg
    24841 gcattaaccc gaatatcagc attgcgctgg gcgggattat cgcctgtggg ctggtttata
    24901 ccgtcattgg cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc
    24961 cggtggtgac gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga
    25021 aaagtgtatc ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg
    25081 ggctggtggc ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggtttaa
    25141 ttgtcgcctg tctgctgtat ggcgtgatga ccaacctttt agggctggga aaagccgtcg
    25201 attttactct cgtcagccat gctgcctggt tcggtttgcc acatttttcc actcctgctt
    25261 ttaatagtca ggcaatgatg ctgattgcgc ccgtggcggt gattctggtg gcggaaaact
    25321 taggtcatct caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc
    25381 gggcattcgt cggtgatggg ctggcgacga tgctttctgg ttctgtcggt ggcagcggcg
    25441 taaccaccta cgccgagaat atcggtgtaa tggccgtgac aaaagtctac tcgacgctgg
    25501 tgtttgtcgc tgctgccgtc atcgccatgc tgttgggctt ttcaccgaaa tttggcgcgc
    25561 tgattcatac cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga
    25621 ttgccgtcgc aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacggta
    25681 atttgattat ggtcgcagta accttagtgc tgggcgcggg tgattttgct ctcacgctgg
    25741 gcggttttac gttgggaggg attggtacag caacctttgg cgcgatttta ctcaatgcgt
    25801 tgttaagcag aaagttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa
    25861 cagaaaaccg ccgggattac cggcggtgat gactttttga cgagcctaag aaaaaaatgc
    25921 attcgtcatt gattagcccg gctagttgtc gcttttgccg tgactgctct tgccaccttt
    25981 tttacctgct tcagatgcgc gctgcgggtc attcttgaaa ttacccccgc tgtgctgtcc
    26041 acctttttta cctgcttctg atgctctttc gcggtcttct gcaaaattgc cggaaccgcc
    26101 tcgatggttt gccattactg acctccgtct gggtatagac atcatattgc atagaattaa
    26161 cgcggcgtat ggcggctgcc atattttcgc tacgtgggac taagcttagt gaactgacgc
    26221 gggcagaccc gcgtttggta atatcctgca acagcttctt agcgagaata gatatcatct
    26281 caaataatta tccataagcc gctcttatga ttaaaaaaac gcgatgagat aaaaatgtgt
    26341 cattttgcga caaaattacg tgcttgtgaa agttgttata aatcaaataa gtggttgtga
    26401 aatttgcact ctgaaaagga cgtcttatct ttaaataagt ggtagcgaat cgctacggaa
    26461 tagagataac acgaggagtg gttagaaatg gctaaagttc tggtgcttta ttattccatg
    26521 tacggacata ttgaaaccat gaagcattaa aaacacagaa ttaccgaaga tcgtccaaga
    26581 gcatatattt ttcaatctgt tacgcctcat tttgaccatg agaaaatcga tttcacaccg
    26641 ttctttatac gttctttttt gccccaaata tgccccaaac aaccacattt tgccccaata
    26701 gtgccccaaa gatttaaggg aatttaagta gatatttcag atgattaacg caagtttacg
    26761 atgtgaattt acgttaattt tctgggtagg gtatttaccg tcattcgttc atcaatattt
    26821 ctgcatattg acaaatacgc ctgagctatc tcgggagaaa tgcaatgaaa gcagcaaaag
    26881 gcgatgttaa tcaccctgct catctctgta ttaccgtcat tgtgacggca ctggtaacta
    26941 agaaagacct ctgcaaggga ggaatccctc gccacctctg atgtgtcagg tatcctcacc
    27001 ctccccaatc acattagccc cgcacttccg tacggggcat gttctttact ccacccccac
    27061 ttttttgttg tactcgcggt taaagcgatc cacggcaacc ttcatatccc gctctaccga
    27121 tttcaccatc gccgactgtt gcgccagact gagagtactg tcggcgtaaa tggcatcacg
    27181 ctgtttacgc aaatccttca gtctcttccg ggtatcctgc ataagcccgt tcatcgacaa
    27241 ttttccgttg ttctcgtcaa tgaacgccgt tctttctgca ccggtcagac tcttcagctc
    27301 tgcgtgatac tgcgcgacct ccgtcatccg gtcgtacatc ttctgctggt cagcatacgg
    27361 catcacctca cccgaaattt tccccaggaa aggcacctgc tgctccggta tatcaatacc
    27421 attcagcgat ttcaccgccg catccgtggt tttggaaatg aagcgccctg tccctccgga
    27481 gatatagtca atccagaatt tcagcgattc cggtgtgata tccaccgcgc cgggacggta
    27541 ctggctgcca cctgagaacg cattcagcca ggatgcaaac gccttgtacg cttctggcgt
    27601 tgaacgtctt cccagctggc tgtcaggttt tggtgtacca aacggcatgt tctcctggta
    27661 aatctgcgcc cccatgaagt tttcattcat ggcaaggttc gcaaacggac gcagaatggt
    27721 cggcgctgca tttttcagca atgccccgga cagtgtttcc gacgtttcac tgcccacagg
    27781 actgaatgcc ccaagcacac caccaacaac attaccggca gcacgtgaag cggtcaggtc
    27841 gcccgctgcc accccttcgg ctgtgtgacc aagcaggaag aaaacgttgt acccgtaagg
    27901 cagaggaata ctccagtact ctccggcctt gcccccgaac accgatttca taatgacgag
    27961 gttacgctct ttcacatgag acggcacctt gtcataccag ttaaccccgt catcatcctc
    28021 acccgccacg ctgcggttaa gcgagccaag cagataaccc gctcccacag cggcaagcgc
    28081 gattttctgc ggaacattca gattcttcca gcgaaggcgc tccagtaaag gtccctctcc
    28141 attaagatgt ccgagcgttc tcaccagatt tgccgttccc tgaatgctgg cgttggcgaa
    28201 catgtacagc gaattcatca gcgccccctg ctcaccacga cggttaaagt tcaccgtcat
    28261 atttttggca agagacgccg cctgctggcg tgacaaaccg gcatcacggg catgtttata
    28321 ggcagaaaga cgcagagcgt tttcaaccgc accgttggca tcctcgacaa ggttaaggaa
    28381 cgaattccat gcaccgatac tctggccttt ccatcccccc ttcgccagtg agacaaggcg
    28441 atccatttcc ttctgctggc cttcaaggtc acccatgtta aaccagccgg ttttccctcc
    28501 gtcctcaaca aactctttcc acaccttctg ccactgtgca ccttttcccg tgagggtttt
    28561 accacgcaga ctggcgtata cggctgacat ggcagaacgg ctgtctttgg ctacagccag
    28621 ggcggataag ttatccagcc ctttcagttt accgtcgctc cttccctgct ccgccttcag
    28681 gttcatcacc gccgtctgca cgtcacggat gaagttactg accaggaatt ccgggttata
    28741 cgacgtgttc accgttgcca ggaagcggtt aactttcccc agcgtacgaa caaaggcatt
    28801 gctggtttcc ggtcccatac tcttcatcgc acgcatcagg cgcggatcat ggagtttgat
    28861 gtagtacgtt ttgccgttct ttttggtggt gaagtaccgg tctgccatca ttgccatcgg
    28921 tacagggcgt tcgacaactt cgcgacgggt ttcaccggtt tccggatcaa cacgctctgc
    28981 aatcgcacgc atggtatcag gtctgtcatc agtgaagacc tgccagtaat ccttgtcagg
    29041 attatcctgc accagtttca ggaaggcgtt acccacttcg tttttgcgat ggcggatcag
    29101 cgattcgctc aggtcctgta tcgcctgagt ggacggagac tgtgcacggg atgcacgccc
    29161 catggcctgc ctgctttcac gtccgccgat ggtgaagccc ttacctgtac ggggcagtga
    29221 caccataccg tcaacatcct gacctttcag gggaacgtag taacggtagg cgttctgcca
    29281 ggcatccacc actccgctct cttccagtcc ggcctcacgg ataagctcac ggcgacgggc
    29341 cagcatatcg tcaacaatcc cagccagacg gtcatactgt gcctgtttgc cactgttacg
    29401 cacacgctgc atgatttccg ccgcttccgc gttggtcatc cccgaaccgc cgtccggcat
    29461 tttcgggttg attttcgcga tatgtgcgtt acgttccggc gcgtgacggg cgtagaggta
    29521 ctcatccaga tcggcctgcg caattttgta gtccgccagt aatttagcca gtggctgaac
    29581 gtagcgctcc ttcatcacgt tcaggtcgtt ttccgccttc ccgtggaaga gttcttccgc
    29641 catataagcg ttgttactat cgtctatttt tccgccagtt ttacggatat tctcctgaac
    29701 agctttcagc acccggaatt tatcctgcat ctggcacaca aaacgcgata taattatctc
    29761 ttccggtgtc agactgctgg tacgggagta atacggcccc ttgcgaatat cttcaggata
    29821 gagtatttta tctacagagc cgcgataaga acgctcctct ttgggcaatt ggagcccact
    29881 gtactgaggg ttatcgcggc ttaatttttc cttccggtaa agcgttaatc cagccatttc
    29941 catgcttttc agctttttcc cgttttccgt accatataca gaagccactt tgttaatttc
    30001 cagacgttta cctgtcgcct tcatgtgcac tgccgacacc accggatcgc catttttatc
    30061 cacggcatca agcagcatca caactgcatt tctttctgtc gcggaacggt aaattgcatc
    30121 cggatcatgc atcagttccg ggagtctctc gataacatcc attggcacca catgtttcac
    30181 gccattggtt gccttccgta cagtgtcgcg ggagataacc agcggcaaat ccggtgcgcc
    30241 aaggtggcgc aataccggcg gcgtacgccc aatgtttgca gtcattgcac cagaacgtaa
    30301 cgacttcatc attctggcaa ggtcatcacg ataacgttca ccctcacctt ccggcacttt
    30361 gaacggatca ggcttaccac tacgggagta ctgagacgat gcgcccacgc catcctcacg
    30421 cggcgtgtaa ccttcccgca cacgctggcc taacgtacgg atggtctcgc gaacaagtct
    30481 gatatcgttc agttccgtcg gcttcagtaa ccccgtacga cgcagtaccc ctttgaccag
    30541 agcaacaaca cgctcccatg ccgccacgaa tttattcggc tgtttctccg ccatatgtgc
    30601 cagaaattca cccgcctgta cttccggaga ttccttacca taggacgcat caaccttacg
    30661 ccatgcttca cggatggtgg cgttatcact gtcgcgggtt ttcagcacgg ttttgataat
    30721 cgtctgatat tccgctggcg tgacaacatg ctccatggca tggtggataa tctcgtgacg
    30781 caacttctcg cgtacggtcc gcccgtcagg gatgttatcc gccaccagga caatttctcg
    30841 tttatccgga cgatagaatg cgtgcaccct gccgtaaccg tcgaacgatt cacccgccag
    30901 cgcttcagcc tctttctgtg acttcaccac acggaccttc aggtcactgt ccttaatgcc
    30961 gctcatcacg ccacgggcaa cagcttcaac ctgcgagacc ggactgcctt tggcttccgc
    31021 actgcggtta acatccgaaa tcagattgcc ttcaggtgtg cgggtcacgc ccttacggga
    31081 ataaaacgca actcccttgt ccgtctcacg ggttttcagt gttcggaaca agtgatcgaa
    31141 tgcctcacga ataccgccat ccagttctgc attcgtcgga taagcgtagg tgtccggctg
    31201 tccatggtca tccgccttac ggatattgac cagataatca ttctccacgc cagccatacg
    31261 cgctttatcc tgaacataac gctcaaaggc tcgcgccgcc atttcaacat ccgttgacca
    31321 gtacggtttt gaacgaacgc catccaggcg ttcagaacga cgcaacatgt cactgctgtt
    31381 aatggctttc atcacgcctt taaacgcgtc gtaaacctcc tgacgtaccg gatattccgc
    31441 atcaacatac ctgccgtctt taaatacgcg cccggcacgc tgtgcttccg tcatatagtc
    31501 gccacctgac gtgatcttcc cgtcagtgga aacatcataa cgaccaaaat aattatccag
    31561 tgaatggaac cattcgtggg ccagcgcccc cggtccgtta ccttttgtca ggttgattgc
    31621 cacctcaccc ggctcatagt gtgccgccgc cttaccctta ccacgggcac caaaagcaag
    31681 cccaagacga ccgttcagag aaagcgcttt tgtcggcacg ttcagcacgt cagccaggtc
    31741 atgcagcgag tcataagccc ggttcaaatc agcctgacga cgcggacttt ccacataatt
    31801 accaaactgc acaccacgaa aaccaaacgc atcactgaac tgctccggcg aaacatcccc
    31861 cttgcggcgt tctggtccgg tacgatcgcg gttggtggcg ttacgctgct cctcacgcga
    31921 gacttcacgc agcgccttca cctgattaac cagctcatca cgatgtgtgc gcacataatc
    31981 atgtgcatca cgggcagact caaaaccgcc cttcagtttc tgcctgtttt tgccataacc
    32041 gataaaaatg tcgccactgg ccttatgccg gtacacgtca aaggttattc tgtcatcagc
    32101 agaggttgct ggcgtttccc tttctctgga gccttgttca tcgaaatatg ctttcgcctt
    32161 tttcagtaac tcatcccggc tttccgagaa aaagaggtcg cgccccttgc tatcacgaag
    32221 cgaatacatc atttgcggcg gatcgtaacg ttttcccccg acaaacgaat aaacaccgga
    32281 cctgacacga tagccggatg ctttatcaat ctgcaatgca gaaagcgtac ggagcagttt
    32341 ccaggtatcc gacatgctcc gtaaccccgg cttactgtcc atcatatcag ccagtttcgc
    32401 tgccggaatt ttcccctcca gcataccggc agtagcttcg cgaccgcttt taacaatgct
    32461 gatccatttc tgcatgttac ggcttgttct ggtgggaatt tcactgcgat acaacgccat
    32521 cattgccagc gtgtcactat ccacaccttc tttttccaga cgagcataat ccggctttgg
    32581 aaacagtttg ctcagtggtt gtgtcgcgta ctccccatcc tccagcgttt tacccatagc
    32641 ttcagcaagc tgcgcataac ggtgttttgc cgcgccctta atctcctcgc caaagtcttc
    32701 aattttcgca ccacgcgcct gctggtgagt gctcggttta tctaccggca cctcagtatc
    32761 cggaacatcg gttatggtct ggcgatcccc ggtctgtctg cgtgcttctg cctgctgtgc
    32821 cagttccgga agggagtcac gaaccatacg agggagtgat tcaggctgtg gcatatcaac
    32881 cccacgctga ctttcaggtg caggaagtcc ttcacgcact ggtgctgtgg cgttctgcgc
    32941 atctggtgac ggcagaccac gacgaaccgg ttcgccctcc agaatttccc cctggcgacc
    33001 agcggcattt tccggtgcct gcgcacgtcc tttctggaaa ctctggccct tcacctcacc
    33061 ggttgtggta aaacgaccac cacgtccggc ctgattttca tccggcgtac gcgccacttc
    33121 tcccggtaac ggatatccct gtccgggatg aatatcgccg ggggcgggca gacgcggacg
    33181 ctcagttaac tcctgtgctg tcggaccggc atcaccttca gccatctgtg aacgtacaag
    33241 ctcctccgcc gtcggcgcgt tgccacgggc aaggcgggcc tgtacctccg tatcatcccc
    33301 cgtgaaacca cggaatcgcg ggtcacgcat gaacgcgggc tgctccatcg ggtcagcatc
    33361 agcaatacgc tctccgtcag ccagggtgtt aatggtttcc gttgccacat cttcagagaa
    33421 cgcaggatta tccatttcaa cggcatactg attctcgcct ttcttcacta cggacggttt
    33481 caggccggt
//
LOCUS       DAZGAN020000045        33171 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGAN020000045 DAZGAN020000000
VERSION     DAZGAN020000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 33171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.045"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(290..2863)
                     /gene="clpB"
                     /locus_tag="ACHKYO_RS11835"
                     /old_locus_tag="pgaptmp_002367"
     CDS             complement(290..2863)
                     /gene="clpB"
                     /locus_tag="ACHKYO_RS11835"
                     /old_locus_tag="pgaptmp_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HGU4429206.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2993..3724)
                     /gene="yfiH"
                     /locus_tag="ACHKYO_RS11840"
                     /old_locus_tag="pgaptmp_002368"
     CDS             complement(2993..3724)
                     /gene="yfiH"
                     /locus_tag="ACHKYO_RS11840"
                     /old_locus_tag="pgaptmp_002368"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HGU4429207.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3721..4701)
                     /gene="rluD"
                     /locus_tag="ACHKYO_RS11845"
                     /old_locus_tag="pgaptmp_002369"
     CDS             complement(3721..4701)
                     /gene="rluD"
                     /locus_tag="ACHKYO_RS11845"
                     /old_locus_tag="pgaptmp_002369"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HGU4429208.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4836..5573
                     /gene="bamD"
                     /locus_tag="ACHKYO_RS11850"
                     /old_locus_tag="pgaptmp_002370"
     CDS             4836..5573
                     /gene="bamD"
                     /locus_tag="ACHKYO_RS11850"
                     /old_locus_tag="pgaptmp_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HGU4429209.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5844..6185
                     /gene="raiA"
                     /locus_tag="ACHKYO_RS11855"
                     /old_locus_tag="pgaptmp_002371"
     CDS             5844..6185
                     /gene="raiA"
                     /locus_tag="ACHKYO_RS11855"
                     /old_locus_tag="pgaptmp_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGU4429210.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6288..6413
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6289..6336
                     /gene="pheL"
                     /locus_tag="ACHKYO_RS11860"
                     /old_locus_tag="pgaptmp_002372"
     CDS             6289..6336
                     /gene="pheL"
                     /locus_tag="ACHKYO_RS11860"
                     /old_locus_tag="pgaptmp_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HGU4429211.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6435..7595
                     /gene="pheA"
                     /locus_tag="ACHKYO_RS11865"
                     /old_locus_tag="pgaptmp_002373"
     CDS             6435..7595
                     /gene="pheA"
                     /locus_tag="ACHKYO_RS11865"
                     /old_locus_tag="pgaptmp_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HGU4429212.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7638..8759)
                     /gene="tyrA"
                     /locus_tag="ACHKYO_RS11870"
                     /old_locus_tag="pgaptmp_002374"
     CDS             complement(7638..8759)
                     /gene="tyrA"
                     /locus_tag="ACHKYO_RS11870"
                     /old_locus_tag="pgaptmp_002374"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HGU4429213.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8770..9840)
                     /gene="aroF"
                     /locus_tag="ACHKYO_RS11875"
                     /old_locus_tag="pgaptmp_002375"
     CDS             complement(8770..9840)
                     /gene="aroF"
                     /locus_tag="ACHKYO_RS11875"
                     /old_locus_tag="pgaptmp_002375"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HGU4429214.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10050..10415
                     /gene="yfiL"
                     /locus_tag="ACHKYO_RS11880"
                     /old_locus_tag="pgaptmp_002376"
     CDS             10050..10415
                     /gene="yfiL"
                     /locus_tag="ACHKYO_RS11880"
                     /old_locus_tag="pgaptmp_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HGU4429215.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGTDENASTIRLN"
     gene            10565..11083
                     /gene="yfiR"
                     /locus_tag="ACHKYO_RS11885"
                     /old_locus_tag="pgaptmp_002377"
     CDS             10565..11083
                     /gene="yfiR"
                     /locus_tag="ACHKYO_RS11885"
                     /old_locus_tag="pgaptmp_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HGU4429216.1"
                     /translation="MRFSHRLFLLLILLLTRAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11073..12299
                     /gene="dgcN"
                     /locus_tag="ACHKYO_RS11890"
                     /old_locus_tag="pgaptmp_002378"
     CDS             11073..12299
                     /gene="dgcN"
                     /locus_tag="ACHKYO_RS11890"
                     /old_locus_tag="pgaptmp_002378"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HGU4429217.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQDIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEISKRLAEVGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12315..12797
                     /gene="yfiB"
                     /locus_tag="ACHKYO_RS11895"
                     /old_locus_tag="pgaptmp_002379"
     CDS             12315..12797
                     /gene="yfiB"
                     /locus_tag="ACHKYO_RS11895"
                     /old_locus_tag="pgaptmp_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU4429218.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12874..13221)
                     /gene="rplS"
                     /locus_tag="ACHKYO_RS11900"
                     /old_locus_tag="pgaptmp_002380"
     CDS             complement(12874..13221)
                     /gene="rplS"
                     /locus_tag="ACHKYO_RS11900"
                     /old_locus_tag="pgaptmp_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGU4429219.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13263..14030)
                     /gene="trmD"
                     /locus_tag="ACHKYO_RS11905"
                     /old_locus_tag="pgaptmp_002381"
     CDS             complement(13263..14030)
                     /gene="trmD"
                     /locus_tag="ACHKYO_RS11905"
                     /old_locus_tag="pgaptmp_002381"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGU4429220.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14061..14609)
                     /gene="rimM"
                     /locus_tag="ACHKYO_RS11910"
                     /old_locus_tag="pgaptmp_002382"
     CDS             complement(14061..14609)
                     /gene="rimM"
                     /locus_tag="ACHKYO_RS11910"
                     /old_locus_tag="pgaptmp_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGU4429221.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14628..14876)
                     /gene="rpsP"
                     /locus_tag="ACHKYO_RS11915"
                     /old_locus_tag="pgaptmp_002383"
     CDS             complement(14628..14876)
                     /gene="rpsP"
                     /locus_tag="ACHKYO_RS11915"
                     /old_locus_tag="pgaptmp_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGU4429222.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15125..16486)
                     /gene="ffh"
                     /locus_tag="ACHKYO_RS11920"
                     /old_locus_tag="pgaptmp_002384"
     CDS             complement(15125..16486)
                     /gene="ffh"
                     /locus_tag="ACHKYO_RS11920"
                     /old_locus_tag="pgaptmp_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGU4429223.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16653..17444
                     /gene="ypjD"
                     /locus_tag="ACHKYO_RS11925"
                     /old_locus_tag="pgaptmp_002385"
     CDS             16653..17444
                     /gene="ypjD"
                     /locus_tag="ACHKYO_RS11925"
                     /old_locus_tag="pgaptmp_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HGU4429224.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17465..18751
                     /gene="yfjD"
                     /locus_tag="ACHKYO_RS11930"
                     /old_locus_tag="pgaptmp_002386"
     CDS             17465..18751
                     /gene="yfjD"
                     /locus_tag="ACHKYO_RS11930"
                     /old_locus_tag="pgaptmp_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HGU4429225.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18806..19399)
                     /gene="grpE"
                     /locus_tag="ACHKYO_RS11935"
                     /old_locus_tag="pgaptmp_002387"
     CDS             complement(18806..19399)
                     /gene="grpE"
                     /locus_tag="ACHKYO_RS11935"
                     /old_locus_tag="pgaptmp_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGU4429226.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19522..20400
                     /gene="nadK"
                     /locus_tag="ACHKYO_RS11940"
                     /old_locus_tag="pgaptmp_002388"
     CDS             19522..20400
                     /gene="nadK"
                     /locus_tag="ACHKYO_RS11940"
                     /old_locus_tag="pgaptmp_002388"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HGU4429227.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20486..22147
                     /gene="recN"
                     /locus_tag="ACHKYO_RS11945"
                     /old_locus_tag="pgaptmp_002389"
     CDS             20486..22147
                     /gene="recN"
                     /locus_tag="ACHKYO_RS11945"
                     /old_locus_tag="pgaptmp_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGU4429228.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22296..22637
                     /gene="bamE"
                     /locus_tag="ACHKYO_RS11950"
                     /old_locus_tag="pgaptmp_002390"
     CDS             22296..22637
                     /gene="bamE"
                     /locus_tag="ACHKYO_RS11950"
                     /old_locus_tag="pgaptmp_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HGU4429229.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22699..22989)
                     /gene="yfjF"
                     /locus_tag="ACHKYO_RS11955"
                     /old_locus_tag="pgaptmp_002391"
     CDS             complement(22699..22989)
                     /gene="yfjF"
                     /locus_tag="ACHKYO_RS11955"
                     /old_locus_tag="pgaptmp_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HGU4429230.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22979..23416)
                     /gene="ratA"
                     /locus_tag="ACHKYO_RS11960"
                     /old_locus_tag="pgaptmp_002392"
     CDS             complement(22979..23416)
                     /gene="ratA"
                     /locus_tag="ACHKYO_RS11960"
                     /old_locus_tag="pgaptmp_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HGU4429231.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23587..24069
                     /gene="smpB"
                     /locus_tag="ACHKYO_RS11965"
                     /old_locus_tag="pgaptmp_002393"
     CDS             23587..24069
                     /gene="smpB"
                     /locus_tag="ACHKYO_RS11965"
                     /old_locus_tag="pgaptmp_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGU4429232.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24284..24646
                     /gene="ssrA"
                     /locus_tag="ACHKYO_RS11970"
                     /old_locus_tag="pgaptmp_002394"
     tmRNA           24284..24646
                     /gene="ssrA"
                     /locus_tag="ACHKYO_RS11970"
                     /old_locus_tag="pgaptmp_002394"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24770..25252
                     /locus_tag="ACHKYO_RS11975"
                     /old_locus_tag="pgaptmp_002395"
     CDS             24770..25252
                     /locus_tag="ACHKYO_RS11975"
                     /old_locus_tag="pgaptmp_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429233.1"
                     /translation="MEELHFVYINANGRIGVHSIQSISYSENHIQGICKNTDRIKTFR
                     KDRILKQYDSPEQAIQECASFLPENYSHLTKQSGPKKNTFDVCFTGFKKADKERLVDK
                     ANEQGLTVRTSVTQSLQMLCCGYNAGPSKVSAARMKGTIIIDEPGFIHFLETGEIPDE
                     "
     gene            complement(25279..25497)
                     /locus_tag="ACHKYO_RS11980"
                     /old_locus_tag="pgaptmp_002396"
     CDS             complement(25279..25497)
                     /locus_tag="ACHKYO_RS11980"
                     /old_locus_tag="pgaptmp_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HGU4429234.1"
                     /translation="MMNCPKCGHAAHTRSSFQVTESTKERYCQCQNINCGSTFVTHET
                     VVRFIVTPALIATAPPHPLPGGQGHMNF"
     gene            complement(25566..26666)
                     /locus_tag="ACHKYO_RS11985"
                     /old_locus_tag="pgaptmp_002397"
     CDS             complement(25566..26666)
                     /locus_tag="ACHKYO_RS11985"
                     /old_locus_tag="pgaptmp_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HGU4429235.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGKDITTVLDKRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHNTTIGEVVKEIAARHKLKMALGKDLSDKPVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDAE
                     WTITTLTHTVSPDNGFTTSIELEVKIDDLEME"
     gene            complement(26663..27148)
                     /locus_tag="ACHKYO_RS11990"
                     /old_locus_tag="pgaptmp_002398"
     CDS             complement(26663..27148)
                     /locus_tag="ACHKYO_RS11990"
                     /old_locus_tag="pgaptmp_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4429236.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEITGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQDTATSALSDISKTVGGLL
                     S"
     gene            complement(27145..30222)
                     /locus_tag="ACHKYO_RS11995"
                     /old_locus_tag="pgaptmp_002399"
     CDS             complement(27145..30222)
                     /locus_tag="ACHKYO_RS11995"
                     /old_locus_tag="pgaptmp_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4429237.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRAAQASSKELAGAIQNTRNSLK
                     ELNKQAGRIDEFRKTRSQLAITANNLNAAREEAAKLATQFAATNRPTAAQAKLFSQAK
                     TRVQELQQTYNGLLGAVQRQRQALKESGIDTRQLSSAQRELKKNAEETRQALEGQKKA
                     LKRLGEQQARMNAAREQYSRRLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQDAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSITGALRRWVEANPELTGTLMKVAAVVAAVT
                     VGLGTLAVVLAAVLGPLAVIRLGFSVLGIKTLPSVTAAVTRTSSALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLASAGTVLRVLASGPLAMLRVALYAVSGLLGALLSPIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPVSAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PERVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAAAPAELPPLHPLALPAKGGEAMVSRAATVPPV
                     HRIEAPTQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            complement(30215..30334)
                     /locus_tag="ACHKYO_RS12000"
                     /old_locus_tag="pgaptmp_002400"
     CDS             complement(30215..30334)
                     /locus_tag="ACHKYO_RS12000"
                     /old_locus_tag="pgaptmp_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HGU4429238.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRYKAIQRSGANDE"
     gene            complement(30349..30651)
                     /locus_tag="ACHKYO_RS12005"
                     /old_locus_tag="pgaptmp_002401"
     CDS             complement(30349..30651)
                     /locus_tag="ACHKYO_RS12005"
                     /old_locus_tag="pgaptmp_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HGU4429239.1"
                     /translation="MSDKQTEKTIQLDTPIKRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            complement(30707..31219)
                     /locus_tag="ACHKYO_RS12010"
                     /old_locus_tag="pgaptmp_002402"
                     /pseudo
     CDS             complement(30707..31219)
                     /locus_tag="ACHKYO_RS12010"
                     /old_locus_tag="pgaptmp_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
     gene            complement(31229..32401)
                     /locus_tag="ACHKYO_RS12015"
                     /old_locus_tag="pgaptmp_002403"
     CDS             complement(31229..32401)
                     /locus_tag="ACHKYO_RS12015"
                     /old_locus_tag="pgaptmp_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HGU4429240.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDVTTLIRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLTDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFSSQVSA"
     gene            complement(32544..33110)
                     /locus_tag="ACHKYO_RS12020"
                     /old_locus_tag="pgaptmp_002404"
     CDS             complement(32544..33110)
                     /locus_tag="ACHKYO_RS12020"
                     /old_locus_tag="pgaptmp_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4429241.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTSEQWAQIGRLLEDGES
                     RQRIALIFDVGVSTIYRKFPANKNNKSP"
BASE COUNT         8103 a         9018 c         7882 g         8168 t
ORIGIN      
        1 gctttttctc agcggcgcgg ggtgtgcata atacgctttc ccgctacaga gtcaagcatt
       61 tatttttgct ttctctgccg gaattctcag gagaaccccg ctgacccggc ggcgtgtttg
      121 ccgttgttcc gtgtcagtgg tggcgcatta tagggagtta ttccggcctg acaagcgaaa
      181 aatataaaaa ctttatcgtt cgctcatttt tcaggcaaaa catcttaaat atagtctttt
      241 ccgtctaact tatagacaaa aacgagcccc gaagggctcg ttttatcatt tactggacgg
      301 cgacaatccg gtcttcatta acttccaggc gaatcacttt acccggaacc aattcaccag
      361 acagtatttg ctgtgccagc gggttttcga tctgctgctg gatagcacgt ttcaaaggac
      421 gtgcaccata gactggatcg taaccgttct cgctcagcag tttcagcgcc tcgtcagaaa
      481 tgtggatttc ataaccacgt tcttccagac gtttgtacag acgtttcaac tgaatctgcg
      541 caatcgaggc aatgtgctgt tcacccagcg gatggaagac caccacttca tcaatacggt
      601 taatgaattc cggacggaag ttatggctta ccacaccgag caccagctct ttcatgtgcg
      661 cataatccag ttcaccgaag cgttcctgaa tcaaatcgga accgaggtta gaggtcataa
      721 tgacaaccgt attacggaag tcgaccgttc tcccctgccc gtcagtcaga cgaccatcat
      781 ccagtacctg caacagaatg ttgaagacat ccggatgcgc tttttccact tcatccagca
      841 ggatgacgga atacggacga cgacgcacag cttcggtcag gtagccacct tcttcataac
      901 cgacatatcc cggaggcgca ccaaccagac gagacaccga gtgtttctcc ataaactcgg
      961 acatatcgat acggaccatc gcctcgtcgc tatcaaacat aaagttcgcc agcgccttac
     1021 aaagctcagt tttccccaca cccgttgggc cgaggaacag gaatgaacca atcgggcgat
     1081 ttggatccgc caaccccgca cggctacggc gaatagcgtt agataccgca tcaaccgctt
     1141 cgttctgacc aatgacgcga tggtgcagtt cttgctccat acgcagcagt ttttcgcgct
     1201 cgctttccat catgcgagaa accggaatcc ccgtccaacg cgccagcact tcagcaattt
     1261 cggcgtcggt cactttatta cgcaacagac gcatagtttt gccttcgagc tgcgttgcgg
     1321 cttccagttg cttttccagt tccgggattt tgccgtattg cagttcagac atccgcgcca
     1381 ggtcccccac acggcgagcc tgttcaatag cgattttcgc ctgttccagt tccgctttaa
     1441 tggtctgcgt accagaaagc gatgccttct ctgctttcca ctcttcttct aactcggagt
     1501 actgacgttc tttgtcgctc agttcttcgt tgagcatatc caggcgtttt ttactggctt
     1561 catcagactc tttcattaac gcctgttgtt ccagtttgag ctggatgata cgacgatcga
     1621 gtcggtcgag ttcttctggt tttgagtcaa tctgcatacg aatgctggat gctgcttcat
     1681 cgatcaggtc gatggcttta tccggcagct gacggtcagc aatgtagcga tgagacaacg
     1741 tcgccgctgc aacaattgcc gggtcagtaa tttgcacatg gtggtgcaat tcgtaacgtt
     1801 ctttcaggcc acgcagaatc gcaatggtat cttcaacaga aggctcggca acaaacactt
     1861 tctggaaacg acgttccagc gcagcatctt tttcgatgta ctggcgatat tcgtcaagcg
     1921 tcgtggcacc tacgcagtgc aattcaccac gcgccagcgc cggtttcagc atgtttccgg
     1981 cgtccattgc gccatcggct ttacccgcgc cgaccatggt atgtaattcg tcgataaaca
     2041 ggatgacgtt gccttcctgt ttggcaagat cgttaagcac gccttttaaa cgttcttcaa
     2101 actcaccgcg atatttcgcc ccagccacca gcgcgcccat atccagcgcc agtacgcggc
     2161 ggcctttcaa tccttccggc acttcgccgt tgataatacg ctgcgccaga ccttcaacga
     2221 tggcagtttt accgacgccg ggttcaccaa tcagtaccgg gttattttta gtacgacgtt
     2281 gcagcacctg aatggtacgg cgaatttctt catcacgacc aatcaccgga tcgagtttgc
     2341 cctgttcggc tcgttcggta aggtcgatgg tatatttttt caaagcctga cgttggtctt
     2401 cagcaccttg atcgttcacg ctttcacctc cacgcatttg ttcaatcgct tgagtaatgt
     2461 tggcggtggt cgcccctgct gctttcagga tgtcggccag cgtgccgcga gactcaagtg
     2521 ccgccagaac gaacagttct gacgagataa agttatcacc acgtttttgc gccagcttgt
     2581 cgcaaagatt aagaacgcgc accagatcct gtgatggctg gacatcacca ccagtacctt
     2641 caacctgcgg taaacgattt aatgcctgat tgatatctgt gcgcaactgg ccagcattta
     2701 tgccagcgga tgttaataaa ggactaaccg aacccccttc ctgattcagc agggcgctca
     2761 ttaaatgaag tggttcgata aattggttgt cgtgcccgag tgcaagtgat tgggcatcgg
     2821 caagagcaag ctggaattta ttagtaagac gatccagacg cataactcct cccataacgg
     2881 atcaaagttg ctactggaga ttaaatgagg tcatccctca attattcaag gttattgacc
     2941 agattaatgt gaaaagaaaa tcacgcgtac cggatcgtct tgattcttta ggttatatca
     3001 gccaaatgaa acttgccata cgaccggtgg tcttgtcgcg acgataagag aagaaagtct
     3061 cattttccgt atatgtacaa cggtcgccgc cgaaaatttg ctcaacaccc acgttcgcca
     3121 gacgctgccg ggcaagctga taaatatccg ccagatactt atcaccatgc tgaatgaaag
     3181 ctgcacttgc tttagcgtct actgccataa acgcctcgcg aacctccacc cccacttcga
     3241 acgcgcgtgg accaattgcc ggccctaacc aggcgagaat attttccgga ttatcagcaa
     3301 aacaggaaac cgtctcttcc agcacgcctg cgcacagtcc acgccagcca gcatgagcgg
     3361 cggcgacttc cgttcccgct cgattgcaaa acagcacagg gaggcagtcg gcagtcatca
     3421 ctgcgcaaac cgtgccgggc gtattgctat aagaggcatc tgctcgtttt gaggcataag
     3481 gttcgccagt aagcttaagc acatctttgc cgtgtacctg ctcaagccag accggtttag
     3541 aaggcaaatt gcccgcagca aaaagtcgct tgcgattctc ctcaacgtga tccgggttat
     3601 cgccacaatg ggcaccgagg ttgagcgagt catacggggg caagctcacg ccgccgatac
     3661 gagaagagct acaggccgca acaccttttg gtagcggcca ctgcgggaca atcagcttac
     3721 tcataaccag tccacttcat ccttatgctc ttcgaaatcg gcgcgcatca cctcaatcag
     3781 ctccaccata tcttgtggaa taggcgcatg ccattccatt tcgatgccgg agatcgggtg
     3841 ataaagacgc agcatggttg catgaagcgc ctggcggtca aacttacgca gcatggagat
     3901 aaatgcttcc gaagcacctt ttggcggacg cggacggcca ccataaaccg gatcgcccac
     3961 cagcggatga gtgatatggg ccatatgcac gcggatctgg tgcgtacgtc cagtttccag
     4021 acgcaaccgc agacgcgtgt gcacacggaa gtgttccatg atgcgatagt gagtcaccgc
     4081 cggtttgccc atcggatgca ccgccatgtg ggtacgtttg gtcgggtggc ggctgatggg
     4141 ttcatctact gtaccgccag cggtcatatg accaatcgcc accgcttcat actcacgagt
     4201 aatttcacgc cgttgcaaag attcgaccaa acgcgtctga gccggaacgg tttttgccac
     4261 aaccatcagg ccagtggtgt ctttatccag acgatggacg atgcccgcac gtggtacatc
     4321 ggcaatgggc ggatagtaat gaagcaacgc attcagtacc gtgccatccg ggttacccgc
     4381 gccaggatgt accaccaggt cgcgcggttt attaatgacg atgatatctt catcttcata
     4441 gacgatatcc agcgggatat cctgcggttc aaaacgcgct tcttcttcaa tctcagcgtt
     4501 gatggcaacc tgctcgccac ccaatacttt ttctttcggc ttatcacaaa ctttgccgtt
     4561 taccagcacg cgctggtcga ggatccattc ttttattcgc gaacgtgaat aatccgggaa
     4621 catttcggcc aaagcctgat ctaagcgttg accgagttgg ttttcggaca ccgttgcagt
     4681 gagctgtact cgttgtgcca tatatactgc ttcttcgttt atcgttgggg ttttacggct
     4741 ttgccgttta atatagtgtg ctattgtagc tggtcttaac cgggagcagg aacagagaat
     4801 ctcccgtatt acattttgag gaaagtcaaa acgtcatgac gcgcatgaaa tatctggtgg
     4861 cagccgccac actaagcctg tttttggcgg gttgctcggg gtcaaaggaa gaagtacctg
     4921 ataatccgcc aaatgaaatt tacgcgactg cacaacaaaa gctgcaggac ggtaactgga
     4981 gacaggcaat aacgcaactg gaagcgttag ataatcgcta tccgtttggt ccgtattcgc
     5041 agcaggtgca gctggatctc atctacgcct actataaaaa cgccgatttg ccgttagcgc
     5101 aggctgccat cgatcgtttt attcgcctta acccgaccca tccgaatatc gattatgtca
     5161 tgtacatgcg tggcctgacc aatatggcgc tggatgacag tgcgctgcaa gggttctttg
     5221 gcgttgaccg tagcgatcgc gatcctcaac atgcacgagc tgcgtttagt gacttttcca
     5281 aactggtgcg cggctatcca aacagtcagt acaccaccga tgccaccaaa cgtctggtat
     5341 tcctgaaaga tcgtctggcg aaatatgaat actccgtggc cgagtactat acagaacgtg
     5401 gcgcatgggt tgccgtcgtt aaccgcgtag aaggaatgtt gcgcgactac ccggataccc
     5461 aggctacgcg tgatgcgctg ccgctgatgg aaaatgcata ccgtcagatg cagatgaatg
     5521 cgcaagctga aaaagtagcg aaaatcatcg ccgcaaacag cagcaataca taacagaaac
     5581 ctgaaataca aaacggcagc ccttgagctg ccgttttttt attctgttag ttgtgaaact
     5641 gaagcgattt agtcactatc gatctcatca aatatggctc gctttgagat attcctcaag
     5701 taaaaaaaca cctcttcctg cgatttctca caaaaaagat tcgttgacaa aaagtgacaa
     5761 aattatgaga ttcccatcac acattttgac atcaggagcg gtatgctgaa ttcaccaaga
     5821 cgggaagaca agaggtaaaa tttatgacaa tgaacattac cagcaaacaa atggaaatta
     5881 ctccggccat ccgccaacat gtcgcagacc gtctcgccaa actggaaaaa tggcaaacac
     5941 atctgattaa tccacatatc attctgtcca aagagccaca agggtttgtt gctgacgcca
     6001 caatcaatac acctaacggc gttctggttg ccagtggtaa acatgaagat atgtacactg
     6061 caattaacga attgatcaac aagctggaac ggcagctcaa taaactgcag cacaaaggcg
     6121 aagcacgtcg tgccgcaaca tcggtgaaag acgccaactt cgtcgaagaa gttgaagaag
     6181 agtagtcctt tatattgagt gtatcgccaa cgcgccttcg ggcgcgtttt ttgttgacag
     6241 cgtgaaaaca gtacgggtac tgtactaaag tcacttaagg aaacaaacat gaaacacaca
     6301 ccgtttttct tcgcattctt ttttaccttc ccctgaatgg gaggcgtttc gtcgtgtgaa
     6361 acagaatgcg aagacgaaca ataaggcctc ccaaatcggg gggccttttt tattgataac
     6421 aaaaaggcaa cactatgaca tcggaaaacc cgttactggc gctgcgagag aaaatcagcg
     6481 cgctggatga aaaattatta gcattactgg cagagcggcg cgaattggcc gtcgaggtag
     6541 gaaaagccaa actgctctcg catcgcccgg tacgagatat tgatcgtgaa cgcgatttac
     6601 tggaaagatt aattacgctc ggtaaagcgc accatctgga cgcccattac attactcgcc
     6661 tgttccagct catcattgaa gattccgtat taactcagca ggctttgctc cagcaacatc
     6721 tcaataaaat taatccgcac tcagcacgca tcgcttttct cggccccaaa ggttcttatt
     6781 cccatcttgc ggcgcgccag tatgctgccc gtcactttga gcaattcatt gaaagtggct
     6841 gcgccaaatt tgccgatatt tttaatcagg tggaaaccgg ccaggccgac tatgccgtcg
     6901 taccgattga aaataccagc tccggtgcca taaacgacgt ttacgatctg ctgcaacata
     6961 ccagcttgtc gattgttggc gagatgacgt taactatcga ccattgtttg ttggtctccg
     7021 gcacgactga tttatccacc attaatacgg tctacagcca tccgcagcca ttccagcaat
     7081 gcagcaaatt ccttaatcgt tatcctcact ggaagattga atataccgaa agtacgtctg
     7141 cggcaatgga aaaggttgca caggcaaaat caccgcatgt tgctgcgttg ggaagcgaag
     7201 ctggcggcac tttgtacggt ttgcaggtac tggagcgtat tgaagcgaat cagcaacaaa
     7261 acttcacccg atttgtggtg ttggcacgta aagccattaa cgtgtctgac caggttccgg
     7321 cgaaaacgac gttgttaatg gcgaccgggc aacaagccgg tgcgctggtt gaagcgttgc
     7381 tggtgctgcg caaccacaat ctgattatga cccgtctgga atcacgcccg attcacggta
     7441 atccatggga agagatgttt tatctggata ttcaggccaa tcttgaatca gcggaaatgc
     7501 aaaaagcatt gaaagagtta ggggaaatta cccgttcaat gaaggtattg ggctgttacc
     7561 caagtgagaa cgtagtgcct gttgatccaa cctgatgaaa aggtgccgga tgatgtgaat
     7621 catccggcac tggattatta ctggcggttg tcattcgcct gacgcaataa cacgcggctt
     7681 tcactctgaa aacgctgtgc gtaatcgccg aaccagtgct ccaccttgcg gaaactgtca
     7741 ataaacgcct gcttatcgcc ctgctccagc aactcaatcg cctcgccgaa acgcttatag
     7801 taacgtttga ttaacgccag attacgctct gacgacataa taatgtcggc ataaagctgc
     7861 ggatcctgag caaacagtcg cccgaccatc gccagctcaa ggcggtaaat cggcgaagag
     7921 agcgccagaa gttgctcaag ctgaacattt tcttctgcca gatgcagccc ataagcaaaa
     7981 gtagcaaagt ggcgcagagc ctgaataaac gccatattct gatcgtgctc gacagcgcta
     8041 atacgatgca gccgagcgcc ccagacctga atttgctcca gaaaccattg gtatgcttcc
     8101 ggcttacgtc catcacacca gaccacaact tgctttgcca ggctaccgct gtccgggccg
     8161 aacatcgggt gtaaccccag taccgggcca tcgtgcgccg ccagcatggc ctgtaatggt
     8221 ccatttttca ctgatgccag atcaaccaga atacaatctt tcggtaaagg cggtaatttg
     8281 ccgataactt gctcagtaac gtggattggc acactaacaa tcaccattcc ggcatcggca
     8341 acaatatcag ccgctcgatc ccagtcatgt tgctccagaa tccgcacctg ataacccgat
     8401 agtgtcagca tcttctcgaa caggcgtccc atctgaccgc caccgccgac gataaccacc
     8461 ggacgcagtg acggacaaag tgttttaaat cctttgtcgt tttcactgga gtaagattca
     8521 cgcatcaccc gacgcaaaac atcctcaatc agatctggcg gtacacccag agcttccgcc
     8581 tctgcacgac gcgaggccaa catagatgcc tcgcgctccg gaacataaat aggcagtcca
     8641 aagcggcttt tcacctcgcc cacttcagca accagttcca gacgcttcgc taataaattc
     8701 agcagcgctt tatcgacttc atcaatttga tcgcgtaatg cggtcaattc agcaaccata
     8761 ataaacctct taagccacgc gagccgtcag ctgcccgttc agatcctgat gaatttcacg
     8821 cagcaaggca tcggtcattt cccagctaat gcaggcatcg gttacggata caccgtattt
     8881 catttcactg cgcggttgct cagaagactg attgccctcg tggatattac tttcgatcat
     8941 cagaccaata attgagcgat tgccatcttt gatttgagca accacggatt ctgccaccgc
     9001 aggctgacgg cgataatctt tattggaatt accgtggctg caatctacca tcagagacgg
     9061 gcgcagtccc gcctgttcca tctctttttc acattgcgca acatccgcag ggctatagtt
     9121 cggcgcttta ccaccgcgca ggatgacatg cccgtccgga ttcccctgag tttgtagcaa
     9181 cgcaacctgc cctgcctggt taatgccaac aaaacggtgc ggctgggcgg cggcgcgcat
     9241 agcgttaatt gctgttgcca gactgccgtc ggtgccgttt ttaaaaccaa ccggcatgga
     9301 aagcccggag gccatttcac ggtgagtttg cgattccgtt gtacgagcac caattgctga
     9361 ccagctaaac agatcgccca ggtattgcgg gctattcgga tctaacgctt ccgtcgccag
     9421 tggcagtccc atattcacca gctcaagcag caatttacgc gcgatctgca gcccggcttc
     9481 tacatcaaaa gagccatcca tatggggatc gttaattaac cctttccagc cgacagtggt
     9541 acggggtttt tcaaaataga cgcgcattac cagatagagg ctatcgctga cctctgcggc
     9601 aagggcttta aatcgacgag catattccag agcagtttcc ggatcatgaa tggaacaagg
     9661 accacatact accagcagac gaggatcgcg cccggcgata atatctgaaa tggttctacg
     9721 cgagtcagca atctgggctt cttgttgcag gctcaatgga aaagcggcct tcagttgttc
     9781 cggagtcatt aaaacctgtt cgtcggtaat atgtacgtta ttcagcgcgt ctttttgcat
     9841 gatggcgatc ctgtttatgc tcgtttgcga tagttgatcc tcagcgagga tgacgtaacg
     9901 ataacacata aagtaaagtt ttcaatccat atttcgtaca tttttcttta cataagcaat
     9961 ttattagcgt tttcaaccct tgcttctgta tagataaatt tacaccccct ttgaaaacaa
    10021 ttcgctatgc tttgaaaaag gagaaagaaa tgatgaaaaa gtttatcgcc cccttgttgg
    10081 ctttactggt tagcggatgc cagattgatc cttatactca cgcgccaacc ttgaccagca
    10141 ccgactggta tgatgttggt atggaagatg cgatatcggg cagcgccata aaagatgacg
    10201 atgcatttag cgattcacag gcggatcgcg gtctatacct taaaggatat gccgaaggac
    10261 aaaagaaaac ttgccagacc gattttactt atgcccgggg actttccggt aaaagctttc
    10321 ctgccagctg taacaatgtt gaaaatgcca gccaactgca tgaagtttgg caaaaaggga
    10381 ctgatgaaaa tgccagcaca atacgattaa attaattaaa accccacaaa taaattaaac
    10441 ataagatttt actcattgtg ttttatttct cacattgatg acgatcgcat gaaaaaatga
    10501 tattttaatg agcattctga aaaaatatcg atgcatttcg agcgaagatg gtggggatcc
    10561 ctgaatgcgt ttttctcacc gactgttctt actgctgatt ctcctgctga cgagagcacc
    10621 aatactcgct caggaacctt ctgacgttgc gaaaaacgtg cgtatgatgg tttccggtat
    10681 tgtcagttac acccgctggc ctgcgctatc agggccacca aagttatgta tcttttcatc
    10741 ttcacatttt agtaacgccc tacaagagaa tggcacgaca tctttgcctt atctacctgt
    10801 catcattcac actcaacaag aggcgatgat ttcaggctgc aatggttttt attttggcaa
    10861 cgagtcaccg acattccaga tggaattaac cgaacaatat ccgtcaaagg cgttattatt
    10921 aattgccgaa caaaataccg agtgcattat tggtagcgcc ttttgtctga tcatccacaa
    10981 taatgacgtc agatttgccg taaacctgga tgccttatcg cgtagcggtg taaaggtcaa
    11041 cccggatgtc ttaatgctcg cacggaagaa aaatgatgga taacgataat tctcttaata
    11101 agcgccccac gtttaaaaga gcattacgca acatcagtat gaccagcata tttatcacta
    11161 tgatgctgat ctggttgctg ctttccgtga cctcggtgct gaccctgaaa cagtacgcgc
    11221 aaaaaaacct ggcactgaca gcagcaacaa tgacttacag tctggaagca gctgtcgttt
    11281 ttgccgatgg ccctgcagca actgaaacac tggcagcgct gggccagcaa gggcaatttt
    11341 caactgcaga agtacgtgat aagcagcaag atattctggc atcctggcat tacacccgta
    11401 aggatccagg cgatactttc agcaatttca taagccactg gctcttccct gcccccatca
    11461 ttcagccgat tcgtcacaat ggcgaaacca ttggcgaagt acgcttaacc gctcgcgaca
    11521 gttcaatcag ccattttatc tggttttcgc tcgccgtact gaccggttgt attctgctgg
    11581 catcaggaat cgcaattacc ctcacccgcc atttgcacaa tggcctggtg gaagcactga
    11641 aaaatatcac cgatgtcgta catgatgtgc gttccaaccg caatttttcc cgacgagttt
    11701 cggaagaacg tatcgctgag tttcaccgct tcgctctcga cttcaacagt ctgctggatg
    11761 aaatggaaga gtggcagctt cgtttacagg ctaaaaatgc gcagcttcta cgtaccgcgc
    11821 tacatgaccc attaaccggg ctggctaacc gcgcagcgtt tcgtagcggc atcaacacgt
    11881 tgatgaacaa ttccgatgcc cgaaaaacgt cggcgttact atttcttgat ggcgataatt
    11941 tcaaatacat caatgatacc tggggtcatg cgacgggcga tagagtcttg attgaaatct
    12001 caaaacggtt agctgaagtt ggcgggctgc gacataaagc ataccgcctg ggcggcgatg
    12061 aattcgctat ggtgctctat gatgtacagt cagaatctga agtgcagcag atatgctcag
    12121 cactgacaca aatctttaat ctcccgtttg atcttcataa tggccatcag accaccatga
    12181 cattaagcat tggttacgcg atgaccattg agcacgcctc tgcggaaaaa ttacaagagc
    12241 ttgccgatca caatatgtat caggccaaac accagcgtgc cgaaaagctg gtgagataac
    12301 aaggatatat cgatatgata aagcacctgg tagcacccct gattttcacc tcactaatac
    12361 tgactggctg ccagtcccct cagggaaagt ttactcctga gcaagtcgcc gctatgcaat
    12421 cttatggatt tactgaatcc gccggcgact ggtcgctggg cttatcagat gccattctgt
    12481 tcgcaaaaaa tgactacaaa ttgctcccgg aaagccagca acagatccaa accatggcag
    12541 ccaaattggc ctcgacaggg ctaacacatg cccgtatgga tggacacacc gataactatg
    12601 gtgaagacag ttacaacgaa ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat
    12661 gggctatggg tggacaaatt ccacgcagca atctcaccac acagggttta ggaaaaaaat
    12721 atcccatagc cagtaacaag accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg
    12781 tgattacaac cccttaaaac acgagcaaac agacaaaaaa agggccagcc aattggccag
    12841 cccttcttaa caggatgtcg cttaagcgaa atcttagtta agacgctctt tgatacgagc
    12901 agccttacca gtacgctcac gcaggtagta cagtttagct ttacgaacag caccacgacg
    12961 tttgacagaa atgctgtcaa ctaccggaga gtgagtctgg aagacacgct caacgccttc
    13021 gccgttggaa attttacgaa cagtgaatgc agagtgcaga ccgcggttac gaatagcgat
    13081 aaccacgccc tcgaatgcct gcagacgttt tttggaacct tcaacaaccc atactttcac
    13141 ttccacggta tcacccggac ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag
    13201 ttgcttaata atgttgctca taatttaatc tcttatcctg ggtaaactga tatctcgggg
    13261 gcttacgcca tcccatcatg tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc
    13321 agcaaccttg cttgctcttc agtcagagcc aggttttcca gaagttcagg tcttctaagc
    13381 caggtacggc ccagcgactg tttcaaacgc cagcgacgta tctcggcatg gttgcccgac
    13441 agtaacactg gcggaacttc catcccttct aacacctcag gccgcgtata gtgcgggcaa
    13501 tccagcaatc cttcagcaaa ggaatcttcc gttgccgagg cttcatgtcc cagtactccc
    13561 ggaataaacc gggaaacgga gtcaatcagc gtcattgctg gtaactcacc accactgaga
    13621 acgtaatcgc cgattgacca ttcttcgtca atttcggttt ggatcacgcg ctcatctata
    13681 ccttcgtagc gaccgcacac cagaatcaat ttttgattcg ttgccagttc gctgacgccc
    13741 gcttgatcaa gcttgcgtcc ctgtggtgac agataaatca cctttgcgcc ttcacccgcc
    13801 gcggcttttg ctgcatgaat ggcgtcccgc aagggttgca ccatcattaa catccccggt
    13861 ccgccgccgt aaggacgatc gtccacggta cggtgccggt catgcgtgaa gtcgcgagga
    13921 ctccagctct ggatgctcag caggccattt ttaactgccc ggccagttac cccgtaatcg
    13981 gtaattgcgc ggaacatttc aggaaacagg ctaattatgc caatccacat agcgccgtct
    14041 tttaccgttt atccggtggt ttaaaaacca ggatcccaat ctacttcgat tgaacgagta
    14101 gtgagatcga ctttcttgat aacctgccca tcgaggaacg gtacgagacg ttccttgata
    14161 ccaaacgcat ctttcaggtt tgccttaatg acgagaacgt cattagatcc ggtttccatc
    14221 atatcgacga ctttaccgag atcgtagcct tcagtggtta ctacctggca gcccatcagg
    14281 tctttccagt agtagtcccc ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt
    14341 tcacaattcg tcagcaggtt cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc
    14401 atgtcctgat tgtggtgctt ccagctttcc agctggactt gctgccactg acccgccttc
    14461 tggataaacc aaggctgata gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact
    14521 ctgagccacc cacgaatacc gtaagacgaa cccatttttc ccaaaacgat gggatcaaca
    14581 ggtgcttgcg cggtgagttg tttgctcatc atgaccaccg tgacagatta agctgctttg
    14641 tttacttctt tgatcagcgc agcaacgcga tcagaaatag ttgcgccctg gccaacccag
    14701 tgagcgatgc gatccagatc caggcgagtg ccttcttctt tttcgctagc gattgggttg
    14761 aagaaaccaa cgcgctcgat gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca
    14821 acctggtaga acggacgctt tttagcgccg tgacgtgcta aacgaatagt taccataaca
    14881 tcctcttgtg tgaataaaac aaccggaccc catcgaggaa cggagtccgg tgtcatatta
    14941 aaagcccgaa aattttactc atttttgcgg gaattgcaat caacagttgc taactctgct
    15001 gtaaaaggcc gtcggcggtg cagccagttt ggtgccggag tgcgcgcagt caccggagcg
    15061 tacacgcagt acgtgaggat gacgagcaca tcccggtgcc aaaatggcaa acaagccagg
    15121 ccgattagcg accagggaag cctgggggca tcataccctt catgcttctc atcatcttcg
    15181 ccattccgcc tttcttcatt ttcttcatca tgcgctgcat gtcgtcgaac tgcttcagaa
    15241 ggcggttcac gtcctgcacc tgcataccgc aaccggcagc gatacgacgc ttacgcgaac
    15301 ctttgatgat ttctggctta gcgcgctctt tcatcgtcat cgagttgatg atggcttcca
    15361 tacgcaccag cactttatcg tccatctgcg acttaacatt atccgggatc tgccccatgc
    15421 ccggcagctt gcccatcagg ctagccatgc cgcccatatt tttcatctgg cgcagttgct
    15481 caagaaaatc gttgagatcg aagccgtcgc cttttttcag cttgctggct aatttctctg
    15541 cctgcgcgcg gtcaactttg ctttcgatat cttcgatcag cgacagtacg tcgcccatgc
    15601 cgagaatacg cgacgcgatg cggtccggat ggaacggctc cagcgcctca gttttctcgc
    15661 caacaccgag aaacttgatc ggtttgccag tgatgtgacg aatagagagc gccgcaccgc
    15721 cgcgggcatc gccgtccact ttggtcaaca ctacgccggt aagcggtaac gcttcattga
    15781 atgcttttgc cgtattggcc gcatcctgac cggtcatggc gtcaaccaca aacagggttt
    15841 caaccgggtt aatcgacgca tggacttgtt tgatctcgtc catcatcgct tcgtcaacgt
    15901 gcagacgacc agcggtatcc accagcagca cgtcgtagaa tttcagtttg gcttctttca
    15961 gcgccgcgtt aacgatatct accggcttct gaccaacatc agaagggaag aaatcaacgc
    16021 ccacctgctc tgccagcgtc tcaagctgtt tgattgccgc cgggcgataa acgtcggcag
    16081 aaaccaccag cactttcttc ttgtgcttct cgcgcaggaa cttaccgagt ttaccaacgc
    16141 tggttgtttt accggcacct tgcaggcccg ccatcagcac gaccgcaggc ggttgcgccg
    16201 ccaggttcag ggtctggttc tcttcgccca tcgccgcaac cagttcgtta cggacgattt
    16261 tgacgaactc ctgccccggc gtcaggctct tattaacttc atgaccaacc gctttctctt
    16321 ttacgcgatt gataaactca cgcactaccg gcagagcgac gtccgcctcc agcagcgcca
    16381 tgcgcacttc gcgcagcgta tcttttacgt tgtcttcagt gaggcgtcca cggccactga
    16441 tattgcgcag cgtgcgcgac aaacgatcgg ttaaattatc aaacattgtc tctcgcctgg
    16501 ggtggaaacg gttggccgca atcgcgacac atcatcagta ttttgccgca gtataacatg
    16561 aaggcgtctt tgttgttatg caacggttgg agcagcgttc acctgacgct atactgcttc
    16621 tctttcttat tgctcaaact gtcgacatca ctatgcccgt ttttgctctg ctcgcgcttg
    16681 tcgcctactc cgtcagtctt gcgctgattg ttcccggtct gctgcaaaaa aacggcggct
    16741 ggcggcgcat ggctattatt tctgcggtca ttgcgctggt ctgccacgca atcgctctgg
    16801 aagcccgcat cctgcccgac ggtgatagcg gacaaaacct cagcctgctg aacgttggtt
    16861 cattggtcag tttgatgatc tgtacggtaa tgaccattgt ggcttctcgc aatcgtggct
    16921 ggttgctgct acccattgtc tatgcctttg cgcttatcaa cctggcgctg gcgaccttca
    16981 tgcccaatga atacatcacc catctggaag caacgcctgg gatgctggtg cacattggct
    17041 tatcgctctt ttcctatgcc acgctgataa ttgccgccct gtacgcgcta caactggcgt
    17101 ggattgatta ccaactgaag aacaagaagc tggcgtttaa ccaagaaatg ccgccattga
    17161 tgagtatcga gcgtaaaatg ttccacatca cgcagattgg cgtggtgcta ctaacgctca
    17221 cgctttgcac tggcctgttc tacatgcaca acttatttag catggaaaat atcgacaagg
    17281 ccgtgctctc tatcgtggcg tggtttgtct atattgtgct gctgtgggga cattatcatg
    17341 aaggatggcg tggacgccgc gtcgtctggt ttaacgttgc gggtgcagtc atcctgacac
    17401 tggcctactt cggcagccga attgtccagc agttaatcag ctaaacccag aaaaggagtt
    17461 tcccctggaa cacatttcta ctactacgtt gatcattatt ctgatcatca tggtggtcat
    17521 ttcagcctat ttttccgggt ccgaaaccgg aatgatgacc ctcaaccgct atcgtctgcg
    17581 acatatggcg aaacagggta atcgctcggc caaacgcgtc gaaaaattgc tgcgtaagcc
    17641 agaccgcctg ataagcctgg tgttaatcgg caataacctg gtcaatattc ttgcctccgc
    17701 gctcggcact attgttggga tgcgtttgta cggcgatgcg ggcgtggcaa ttgcgactgg
    17761 tgtgctgact tttgtcgtac tggtttttgc tgaggtattg ccgaaaacca ttgccgcgct
    17821 atacccggaa aaagtcgctt atccgagtag ttttctgctg gctccgctgc aaattttgat
    17881 gatgccgctg gtctggttgc tgaatgctat cacccgtatg ctgatgcgca tgatgggtat
    17941 caaaaccgat atcgtggtta gcggctcttt gagcaaagaa gagttgcgca ctatcgtgca
    18001 cgaatcacgc tcacaaattt cccgtcgcaa tcaggatatg ctactgtctg tgctcgatct
    18061 ggaaaagatg accgttgatg acatcatggt gccgcgcagt gaaattatcg gtattgatat
    18121 caacgatgac tggaaatcga ttctgcgcca actctcccac tcacctcacg ggcgcatcgt
    18181 gctctaccgt gattcgctgg acgacgccat cagtatgctg cgagtacgtg aagcctggcg
    18241 gttgatgtcg gagaaaaaag agttcaccaa agaaaccatg ctgcgcgccg cggacgagat
    18301 ctattttgtg ccggaaggta cgccgctcag cacgcagttg gtaaagtttc agcgcaacaa
    18361 aaagaaagtc ggcctggtcg tcaacgagta tggagacatt caggggctgg tgacggttga
    18421 agatattctg gaagagattg tcggcgattt cactacgtcg atgtcgccaa cacttgccga
    18481 agaggtcacg ccgcaaaacg acggttcggt gattatcgat ggcaccgcca acgtgcggga
    18541 aatcaacaaa gcctttaact ggcatctacc ggaagatgat gcccgcacgg ttaatggcgt
    18601 cattcttgag gcactggaag agatccctgt cgcaggcacc cgcgtgcgta ttggcgagta
    18661 cgatatagat attctcgacg tacaggacaa tatgattaag caggtaaaag tttttcctgt
    18721 gaaaccgctg cgcgagagtg tggcggagta acgaaaacgg ccctgcattc gcatgcaggg
    18781 ccgtgaatta ttacgaaaac agaaattacg cttttgcttt cgctacagtt accatcgccg
    18841 cacgaatcgt acgaccattc agcgtataac ccttctgcat aatgcccagt acgttacctg
    18901 gcgcaacgtc atcagattcc accattgcga tagcctgatg cacattcggg tccagcggca
    18961 cgttagtttc ggcgatcact tcaacaccaa acttacgcac aacatccagc atcgacttca
    19021 gcgtcagctc aatgccttca accatcgcag acatatccgg gttagcttta tcagccactt
    19081 ccagcgcacg atccaggcta tcaatcaccg gcagcaattc gttgatgaat ttctccagcg
    19141 cgaatttgtg ggctttttca atatccagtt cagtacgtcg acgcaggttt tccatttcgg
    19201 cttttacacg caaaatgccg tcacgttcac gggtctgggc ttcagccagc tgagcttcga
    19261 gattcgcaat tttttcatcg cgcggatcca cctgctcagc agaagcttct ggctcaactg
    19321 cctcaatctc ttcgtgctga tccatgataa tttcttccgg ggcttgcccc tcaggcgttt
    19381 tctgttcttt actactcatg aatttctccg cgtttttttc gcattcatct cgctaacttc
    19441 gcttattatg gggatcagtt tcagggtttc aagggaagca ctcacattgt catcaatctt
    19501 cgcaacaagg acctcggaaa aatgaataat catttcaagt gtattggcat tgtgggacac
    19561 ccacggcacc ccactgcact gacaacacat gaaatgctct accgctggct gtgcacaaaa
    19621 ggttacgagg tcatcgttga gcaacaaatc gctcacgaac tgcaactgaa gaatgtgaaa
    19681 actggcacgc tcgcggagat tgggcaacta gctgatctcg cggtagtcgt tggtggcgac
    19741 ggtaatatgc tgggcgcggc acgcacactc gcccgttacg atattaaagt tattggaatc
    19801 aaccgtggca acctgggttt cctgactgac cttgaccccg ataacgccca gcaacagtta
    19861 gccgatgtgc tggaaggcca ctacatcagc gagaaacgtt ttttgctgga agcgcaagtc
    19921 tgtcagcaag attgccagaa acgcatcagc accgcgataa atgaagtggt gcttcatcca
    19981 ggcaaagtgg cgcatatgat tgagttcgaa gtgtatatcg acgagatctt tgcgttttct
    20041 cagcgatctg atggactaat tatttcgacg ccaacaggct ccaccgccta ttccctctct
    20101 gcaggcggtc ctattctgac cccctctctg gatgcgatta ccctggtgcc catgttcccg
    20161 catacgttgt cagcacgacc actggtcata aacagcagca gcacgatccg tctgcgtttt
    20221 tcgcatcgcc gtaacgacct ggaaatcagt tgcgacagcc agatagcact gccgattcag
    20281 gaaggtgaag atgtcctgat tcgtcgctgt gattaccatc tgaatctgat tcatccgaaa
    20341 gattacagtt atttcaacac attaagcacc aagctcggct ggtcaaaaaa attattctaa
    20401 ttttacgcca gcctctttac tgtatataaa accagtttat actgtacaca ataacagtaa
    20461 tggtttttca tacaggaaaa cgactatgtt ggcacaactg accatcagca actttgctat
    20521 cgttcgtgag cttgagattg attttcatag cggcatgacc gtaataactg gcgagaccgg
    20581 cgcgggtaaa tctattgcaa tagatgccct cggtctttgt ctcggtggtc gcgctgaagc
    20641 cgacatggtg cgtaccggcg ctgctcgcgc tgacctgtgc gcccgttttt ctctgaaaga
    20701 tacgccagcg gctctgcgct ggctggaaga aaatcagctt gaagacgggc atgaatgttt
    20761 gcttcgtcga gtgatcagca gcgatggtcg ctcccgtggt ttcatcaacg gtacagctgt
    20821 tcctctgtca caactgcgcg aactgggtca gttgctgatt cagatccatg gtcagcacgc
    20881 tcatcaatta ctcaccaaac ctgagcacca aaaattcctg cttgatggct atgccaatga
    20941 aacctctcta ctgcaggaaa tgaccgcacg ttatcagttg tggcatcaaa gctgccgtga
    21001 cctcgcgcat catcaacagt taagtcagga acgcgccgcc cgtgcggaac tgctgcaata
    21061 ccaattaaaa gaacttaacg aatttaatcc gcagcccgga gagtttgaac aaatcgacga
    21121 agagtacaaa cgtctggcga acagcggtca attgctgacc accagccaga atgcattggc
    21181 attaatggcc gacggtgaag acgcaaacct gcaaagtcag ctttacacgg ctaaacaact
    21241 ggtgagcgaa ttgattggca tggacagcaa actgtccggc gtacttgata tgctggaaga
    21301 agctaccatc cagattgctg aagccagcga tgaactgcgc cactactgcg atcgtctgga
    21361 tctcgatccc aaccgactat ttgaacttga acagcgcatc tcaaaacaga tttcgctggc
    21421 acgtaaacat cacgtcagcc ctgaggcatt gccacagtat taccagtcgc tactggaaga
    21481 acagcagcaa ctggacgatc aggccgactc acaagaaacg cttgcgctgg cggtaacgaa
    21541 acatcatcag caggcactgg aaatcgcgcg cgcattacac caacaacgcc agcaatatgc
    21601 agaagaactt gcacagctga tcaccgacag tatgcatgcg ctctcaatgc cgcatgggca
    21661 gtttacgatc gatgttaaat ttgacgagca tcacctgggc gctgacggtg ccgatcgtat
    21721 tgagtttcgg gtaaccacca acccaggtca gccaatgcag cctattgcca aagtcgcatc
    21781 cggtggtgaa ttatcccgca tcgcactggc aatccaggtc atcacggcgc gtaaaatgga
    21841 aaccccggca ctgatttttg atgaagtgga tgtagggatt agcggtccaa cagcggcagt
    21901 tgtcggcaaa ctgctgcgtc aactcggcga atcaactcag gtgatgtgtg ttacccacct
    21961 gccacaagtc gcgggatgtg gtcatcaaca ctattttgtc agcaaagaaa ccgatggtgc
    22021 gatgacagaa acgcatatgc aatccctgaa taaaaaagcg cggttacaag agctggcgcg
    22081 cctgcttggt ggcagtgaag tcacacgtaa tacactggcg aatgcgaaag aactgcttgc
    22141 agcgtaaact tttttcctgc ttcacggtca gagtaaacag caaaacgccg taagaccgga
    22201 aagcaaaagg ttttaaagtg atgaaaggtc tattatcatc ggcatattac agatgagcca
    22261 cgtactgctc gggcccgaaa aggaatcaaa tcactatgcg ctgtaaaacg ctgactgctg
    22321 cagcagcagt actattgatg ttgaccgcag gctgttccac tctggagcga gtggtttacc
    22381 gtcctgacat caaccagggg aactatctga ccgctaacga cgtatccaaa atacgtgttg
    22441 gcatgacgca acaacaagtt gcgtacgcat tgggtacacc gctgatgtcc gatccatttg
    22501 gtacgaatac ctggttctat gtcttccgcc agcaaccagg tcatgaaggt gtaacgcagc
    22561 aaacgctgac gctgaccttt aacagtagcg gtgtgttgac caatattgat aacaaacctg
    22621 cgctgagtgg taactaataa taaagttgct ctcaaagacg ttaaaaaagg tgctcaatga
    22681 gcaccttttt tctgtctgtt atttattcgc tgatttttct gctcgttgcc tgcgaagctc
    22741 tttcggatcg gcaatgagag ggcgataaat ctccacccga tcgccatcat gcacactatc
    22801 gcttagtttt gccggacggc tgtaaatgcc gactttgttt ttagttaaat cgatatcggt
    22861 acgcaattcc agcaagccac tggcgcgaat agcttcttca accgtcgcgc cctcctgcag
    22921 cgtcacgcgc tgcaggtact gcttctcagg tagcgcataa gccacctcaa cggcaatttt
    22981 acctggcact gtaaacctct ttcgcacgaa ccgtaaaagc ctggaccata ttagacgcca
    23041 gctctttaaa cacgcgacca aaggcgagtt caatcaactt attggtaaac tcaaagtcga
    23101 gatgaaactc gatacgacac gcctcctggc tcagcggcgt aaacttccat ccaccaatca
    23161 atttcttgaa cggcccatcc accagattca taagaatact ttggttactg gtcaactggt
    23221 tgcgggtagt aaacgttttg ctgatcccag ccttagagac atctaccgcc gcagtcatct
    23281 gcccaggagt ggactccaga atccgacttc cggtacaacc cggcaaaaac tgaggataag
    23341 actgaacgtc attcactaac tgatacattt gctccgcgct gtagggtacc agtgcggtcc
    23401 ggctaatctg aggcataaca atttccatca acaaaaatcc aacaaataat atcatttatc
    23461 ctgctgtaaa aaaaacgcta tcccggcgct gggtaacatc gggttcatgc taagatagag
    23521 ccttgtcccc cgcaggattg atatggggtg ttttcgattt cagattaccg atgattcacg
    23581 acgcttatga cgaagaaaaa agcacataaa cctggttcag cgaccatcgc gcttaacaag
    23641 cgcgcccgtc acgaatactt tatcgaagaa gagttcgaag cgggacttgc cctgcaaggc
    23701 tgggaggtta aatccctgcg cgcaggaaaa gccaatatca gcgacagcta cgtccttctg
    23761 cgtgacggag aggcatttct gtttggcgct aacatcacgc caatggccgt ggcctccacg
    23821 catgtggtgt gcgatcctac ccgtacccgc aagttacttc tcaaccagcg cgaactggac
    23881 tcattgtacg gtcgcgtcaa tcgagaaggc tataccgtag tggcgctctc cctgtactgg
    23941 aaaaatgcct ggtgcaaagt gaaaatcggc gtcgccaaag gtaaaaaaca gcacgataaa
    24001 cgttcagata tcaaagaacg cgaatggcag gtggataaag cacgtatcat gaaaaacgcc
    24061 caccgttaaa cctgcactcc aattattgac cagttcctca ccgcgcctcc ctctccggcg
    24121 gcgcgaatga acatcttatt ggctatcaca tccgacacaa atgttgccat cccattgctt
    24181 aatcgaataa aaatcaggct acatgggtgc taaatcttta acgataacgc cattgaggct
    24241 ggtcatggcg ctcataaatc tggtatactt acctttacac attggggctg attctggatt
    24301 cgacgggatt tgcgaaaccc aaggtgcatg ccgaggggcg gttggcctcg taaaaagccg
    24361 caaaaaatag tcgcaaacga cgaaaactac gctttagcag cttaataacc tgcttagagc
    24421 cctctctccc tagcctccgc tcttaggacg gggatcaaga gaggtcaaac ccaaaagaga
    24481 tcgcgtggaa gccctgcctg gggttgaagc gttaaaactt aatcaggcta gtttgttagt
    24541 ggcgtgtccg tccgcagctg gcaagcgaat gtaaagactg actaagcatg tagtaccgag
    24601 gatgtaggaa tttcggacgc gggttcaact cccgccagct ccaccaaata aatcaagggg
    24661 ttacgtgaaa gcgtagcccc tttttctttg gtagtggcgg caaaatggcg acagactttt
    24721 gcgtccatct tgcctgtcgc catcttgaaa tcatgcaaag aggtttcaca tggaagaact
    24781 tcactttgtt tacatcaatg caaatggtcg tatcggtgtt cactctatac agagcatcag
    24841 ttatagcgaa aatcatatac agggcatttg taagaacacc gatcgaataa aaaccttccg
    24901 aaaagaccgc attcttaaac agtacgattc accagaacaa gccattcagg agtgcgcgtc
    24961 attcctcccc gaaaactact cacatctcac caagcagtct ggtccgaaaa aaaatacatt
    25021 cgatgtatgt ttcaccggat ttaagaaagc agataaagaa agattggttg ataaggcgaa
    25081 tgaacaagga ttaacggtaa gaacctctgt aacccaaagc cttcagatgc tctgttgcgg
    25141 ttacaatgca ggcccatcaa aagtatcggc agcccggatg aaaggcacaa tcatcataga
    25201 tgagcctggc tttatacatt ttcttgaaac gggtgagatc ccagatgaat aaaaacctgc
    25261 cgtagcaggt tctctttctc aaaaattcat atgcccctga ccacctggca atggatgtgg
    25321 aggagcagta gcaatcagtg cgggtgtcac aataaaccgg accactgttt catgggtaac
    25381 aaaagtgctc ccgcagttaa tattttggca ctggcagtaa cgctctttgg tgctttcagt
    25441 tacttgaaaa ctgctccttg tgtgtgccgc atgaccacac tttggacaat tcatcatatc
    25501 cagatcccta cctttgctat cagaatcatt gtaatgatac acaaaatatc aatattgaga
    25561 acactttatt ccatttcaag atcatcaatc ttcacttcga gttcaatact ggttgtaaaa
    25621 ccgttatccg ggctgacggt atgtgtcaga gtcgtaatgg tccattccgc atcatctatc
    25681 ggctgtttaa aaccactgac cttcacaggc atttccgtgt agagatctgc ccgcccttcc
    25741 gccagttgta gcgagaatga cgcaacgcca cgttgcaggc gttcccactg cattttcgct
    25801 gcccgttcag cgttgctccg gttggcataa gtgcgattaa gtaccagcac gttttcatcc
    25861 gtccccacca ggtaatcgcc ctgcttcgct tccggctctt tcttctgctt cttagtcctg
    25921 cgcttacgct tcaccgtagt gctttctttc ttcgcgggtt cgcgggtatg caaccagctg
    25981 gcaattaccc ccgtgtaagc tccgcgatct gccagggtaa agcggtgact gtcgccgtcc
    26041 ttacgtgtga tagtaatcac cggcagtggt ttaccgctgg cgcttttgcc ctgtccctgc
    26101 cggatgaata acagattgcc atttttcacc gatgcaatgg caccgtactg gcgcgccagc
    26161 cgcatcaaaa aactgccgtc actctcatta gtctggtcta tatgctccac aggcttatcc
    26221 gacaggtctt tacccagtgc catcttcagc ttgtgccgcg cggctatttc cttcaccact
    26281 tccccgatgg tggtgttatg ccacgatttt tcacggcggg tattcagcgt ttcccgaaaa
    26341 tcagcacttc gcgcccggat agtcaggcgg tccggtgcgc cagtgtgttc aatctcgtcc
    26401 accgtgaatg cccctttcgg gaaaagcggc tgccccttcc agcccagcgc cagcgtaatg
    26461 accgcaccac ggcgcggcag cacgattttt ccgtcggcgt cgtccagctc cagatcaagc
    26521 tggtccgctt caaagccccg gttatccgtc agcgtaagcc ccatcaggcg tttgtccagc
    26581 acagtggtga tatccttccc ctcaatactg atgctgaatg cgggagtttt gttgcctttg
    26641 ttaagcagtt cagagctgaa attcacgaca gcagccctcc caccgtttta ctgatatcgc
    26701 ttaaggcaga cgttgccgta tcctgcaaat tattcagctg cgcactgaga tcaccgaaca
    26761 tatcggacag ggattcatcc acccgtttga gcgacagggt aaactcaatc cggcgcggca
    26821 taccatcgcg gaaaaactcc gttttagtct gattcagtcc ctcaatcaca tacatgccgt
    26881 aaatcgtgcc gctgccttca atcaggggcc atgcttttcc ctgttctgcc atctgctcca
    26941 gagccagcaa cgacagcctg ccgcccgtta tctccggcat aagaacacca gaaagcgtca
    27001 gcatgtcgtt gtccggtccc agaaactgcg tggacggacg acggtttacc cggctgtttg
    27061 ccgcatgtcg ccagctgcgt tgatactgca gttcctgata cggaacggtg cgcagcataa
    27121 acacgtacaa tcccagcacc atcatcatgc gtcgtatccc ccctgatcgc tgtagttact
    27181 cctggctttt gccttcagcc tgcgttcacg ttcatcaagc tggcgtgcca cctcccgcgc
    27241 aatatcctgc gcactttgtc ctggctgcgt ctgaatgatg atctgcgtcg gtgcctcaat
    27301 ccggtgaacg ggcggcacag tggctgcacg actcaccatc gcttcgccgc ctttcgcggg
    27361 aagtgccaaa ggatgcaacg gtggaagctc tgcaggcgcg gcagcaacgc ccatcattcc
    27421 ggcaacaacg gcagccagtg cagctgtatt tctccggctg gtcacatttg ccgggccgtt
    27481 aacaatttcc ggcccgtttt caccgacgat gccaaactgc ccacgcggga tatagccgcc
    27541 gctgtcatac atccccgcaa agccatatcc ccatgacgga aaaccccccg atggcatcat
    27601 cactttaccg tctgcattca ccgtcgcagg ttgctgacgc gtcactcttt ccggcagttt
    27661 cgcctttgca gcctctttac tgacaatgcc gagtttctcc agcaaccagg aaacgccgga
    27721 tttcagggag tccagcggat gcatgaccat attcagccct tccgccagtg cctccccgaa
    27781 tcgccgcccc attgccgctg cactctgcag ttcggcagag gtcgacttaa cgggcgtcag
    27841 cagatcagta aaccagcccc acagcgcctg tactttgtcg ccaatccact ggaacacggg
    27901 cttaagcggt tcgaatgctg cactgacggg acctgccgcc gctttgaatc cttccaccac
    27961 gccaccaaga aatgcggtga tgggttgcca gtatttccag acaaccagcg ccacgcccgc
    28021 cagtgcagta accacaagac ctatcggact gagcagagca cctaacagac cagatacggc
    28081 atacagggca acgcgcagca tcgccagtgg accagatgcc agtactcgca gcaccgtgcc
    28141 tgcggaggcc agtccaccgc gcagtaccgc cagaggattc ataaacatca cagcaacagc
    28201 acgtaaaccg gataatccag accgcaaaag tgcaaccggc gcacctgcta cagttttcag
    28261 gacatttccc gtcagtgatg ccgtgcggcg caaagacgac aacggcgcag taagtaaacc
    28321 tgcggcgttg cccgatgaag caagcccgcg tcgcagcagt gcaagtggtg cgccagccaa
    28381 ccaggacaac gcgctgctgg ttcgcgttac tgctgccgta acggaaggta acgttttgat
    28441 acccagcaca gagaatccca gacggatgac tgccagcggc cccagcacag cagccaacac
    28501 cacagccagg gtgccgaggc ccacagtaac agcagccaca accgcggcta ctttcatcag
    28561 tgtgcccgtc agttcagggt tagcttccac ccagcgacgc aacgcccccg tgatgctttt
    28621 caccgtgtac agaatatcca tcagcggctg gcgcagcgtt tcgcccaggc tgctgaaggt
    28681 gttctgcgct ccggttttga ccaacaacca ctgcgcagaa agtgagtcct tgttaatgtc
    28741 ggattctttc tgcatcgaac cgagcgcatc attgcccgct gtcagtttta gctggcgctg
    28801 cagttccggc aggttgtttg ccagtttcgc cgcatcatcg ccaaactctt taccaaacaa
    28861 catggtcatg gcagacaggc gcttatcctg cggcagtgcg ttcaccttct ccagtacgcg
    28921 ctggatagtt cccatcgcat ccttcgtcat ctgcttttca atcacttcag gattgagttt
    28981 cagcagattc attccttcaa agaaactctt gctttgcatg gtcgcaatgg acaattcacg
    29041 caccatcgcg ttggcagcac tggcagcgac ctccggcgca gcacccagtg tcaggaaggt
    29101 ggaacccagc gccgccgctt tacgataatc cagacgatca gccacaccgc ccaggcgttg
    29161 catgacatca atgatgtctg cccctttcga catagcgtta tcatccagat agttcagcgc
    29221 atcaccgagc tgttcaatat tgcgggttgg tattttgtag agctgggcga ttttccccag
    29281 actttctgac agttcatcag ctggcagctc aaaggctgtt gccgcctttg ctgccgtact
    29341 ggcgaaggcc agcaggtcac gtttctggtc ctcccagctg tcgtcagggt ttgccacatt
    29401 catgcgcgca ccaccttcaa ccagtgcggc gaagtccaca gcaccgtttt ccattggcag
    29461 ctgttcactg gcagctttga tggcatcctg catttcgtaa aaacgcgcag tgcggttgcc
    29521 attatcgtca cgcagaccat tgacctgctt tgccacacct ttcatggcat cttccatgct
    29581 ggtatagctt tttaccgccg ccatcactgg cgcgcccatt gccagccctg cagccgtggt
    29641 ggtggctccg gctcctgcga tgcgatcgcg cacttcaagc cgtcttgagt attgttctct
    29701 ggcagcgttc atccgtgcct gttgttcacc cagacgttta agtgcttttt tctggccctc
    29761 cagtgcctga cgagtttctt cggcattttt cttaagttct cgctgggcac tactgagttg
    29821 tctggtatca atccctgatt ctttaagtgc ctgacgttgt ctctggaccg cccccaacaa
    29881 gccgttatag gtctgctgaa gttcctgtac tcgtgttttg gcctgactga ataactttgc
    29941 ctgcgcggcg gttggcctgt tagtggcagc aaattgtgtg gcgagttttg ccgcctcttc
    30001 gcgggctgcg ttcaggttgt tggctgttat ggctagttgc gagcgcgtct tgcgaaattc
    30061 atcaattctg ccagcctgct tattcagttc tttgaggctg tttcgggtat tctgaattgc
    30121 gccagccagc tctttcgaac tggcctgtgc agcacggaat gggcgggtga gtttgtcaac
    30181 cgcattaaga atgacctgca ggcgcaggtt attatcactc atcgttggcc ccgcttctct
    30241 gaatcgcttt ataccgccat tccagcactt cggtcagcgg cataacgtca gtaacggatg
    30301 gcggccagtg aaagatggtg gcgatatcag ccaccagatc gtcaaccgtc aggctgtcgg
    30361 taaaccggca agcaccgact tcttcaacaa aaaagtgaca acctcaaccg acatggcagt
    30421 gagatctgcc gggtccatct ctgcaatttc ctgtgcagtc agtgccggac tggagatgcg
    30481 ggggatcacg gtcatcatcg cgttcacatc catatccata atggcctgca ggcgtgtacc
    30541 gcgcagcgca ccggactgcg gtttacgcag cacaatttcg gtgatttctg ttttaccgcg
    30601 cttgatgggg gtatccagtt gaatagtctt ttcagtctgc ttatcgctca ttttgctgtc
    30661 ctgtaaattg ggttctggcg cggtatcccg cgccgttcag atacatcaga ggcagggcgt
    30721 tgcggtgcgc ttccatcagg tccacaccgt ccacaatttc caccatgttg ataaggtcca
    30781 cttcatagag cacctcacca tttatagtca gcttcgcgta gctgttggta ctggtcactt
    30841 tggtggtgtt gctttcgccc gtcttccact cgccggaatc cacttctttg tgacgtccac
    30901 gcaccacaag ctccacggcc tgcacttccc cggtatcgtc acgctggata gagccggtaa
    30961 agcgcaactg gataccatcc accgtggctt tgcccatctg cttaaacagc agcagttcag
    31021 taccaccaat ggaaaattcc gtatccagtg cgccgtcatc cagccccaga tccacatcca
    31081 ctgcacccgg cattccgccg ccgcgatact tctcatattt gcgggtaaat ttcggcagcg
    31141 tcagcgactc aacgatcccc tgccagttgt tcccgtcatt aaacaggttc aggtgtttta
    31201 atttgcgtgg taaagccatg ttgtcccctt acgcgctgac ctggctggag aaattaacca
    31261 ggtactgatc ggtgatgcgc tgacgcagca tcaggttttc aagtggcggc actggcgtgt
    31321 agtcgtagtc gatggtgagt tttccggctt tcagcgtgtc tttgtcgttc accgactcat
    31381 ccagccagca atcaccacca atgagatagc cctgactgac caggctgcgc attttggcgc
    31441 ggataccctc gataatgtcg cgggccagcg acggattcag cggtttatcc accgcccaca
    31501 tgtgcgcttc tgccatcgtg tccgtcagca cctgcgccgt gcgggtgtag ttttcgaagg
    31561 caaagagcgg gtcatcactc aggcagcggg aaccccagaa gcgaaaaccg tctttgcgga
    31621 taagggtggt gacgtcgttc tggttcagca gtcccgcatc ggttgccggg tcctgcagat
    31681 cccagaacac atcagcagaa attccggtga caccgttaac gcccacgttg gacaggcttt
    31741 tgtgccatcc ggtctgctcg tcaattttgg cgcgcagacc aagcgcacgg gcggtggcat
    31801 aagccgttgc ttcggcattc agcaccgtgt cccagccagt aaagtcgggc cagatcagca
    31861 ttccttcacg ctgactgaag ttttcacggt aagtgattgc ttcctgcacc gtcttgcaac
    31921 catacgctga caggtaagca aatccacgca ggctttgcgc cacactcagc aactcagtag
    31981 ctaccgcctt cgtgtcgtga cctggcacgc cgagaatgcg cggtttaacg ccgagctgtg
    32041 actgggcaga taacagggct ttcatgcctg tttttttacc ttcagcggtc actgcgccga
    32101 tgatattggt cgtggtttct tcttccgttt caccctgcgg cacacgcaca acaacggtca
    32161 cgggttttgc ctggtcagcg atggcatcca gcgaacgagc cagcgtgcct gactcacccg
    32221 ctttaccgct ggcagtcagc acatcagtga tcagcacggg tttattaaga ggaaacattt
    32281 ttgcatcggc atcatcgccc gtgcagacca tacccacgat ggcggtgctc accgtggtaa
    32341 tggatcgggt gccttcgttg acttcaacaa cgcgcacccc gtggtggtaa tcctgagcca
    32401 tagtggcgaa cctcctgatt ggattaggct tcgccctatg ttgaagtgat tgtgcctgac
    32461 aaacagctaa gcgcagttgt accgttattc acacaaaatg acggtatttg tctgctttca
    32521 gggataatca aaataaagct gattcaggga gatttattgt tcttatttgc cggaaatttt
    32581 cgataaatgg tagaaacgcc tacatcaaaa atcagtgcaa tacgctgtct tgattctccg
    32641 tcctcgagta aacgcccaat ctgtgcccac tgttcgctgg tcaacttagg acggcgtcca
    32701 cctactctgc ctttagcacg agctgcagcc agccccgccc tggtacgttc aactatcagt
    32761 tcgcgttcca tttcagccag ggcacccatg acatgaaaaa agaaacggcc cattggggtg
    32821 ctggtatcaa tactgtcagt caggcttcgg aaattcacgc cacgctggcg cagctcttct
    32881 atcagcgtaa caagatgccg catactgcgc cccagtctgt ccagcttcca gacaaccagc
    32941 gtgtctcctg ccgatagtgt cctgagcagc tttttcagcc ccggtctgtc ggacttggtg
    33001 ccactgattt tatcctcaaa aatccgctca catcccgcac agttcagtgc attgcgttgc
    33061 aaatcggtgt tctggtcatt tgttgacacg cgtacatagc caataagcat gatcatcccc
    33121 ctgaataaaa accggagatg atgccagtta gccgttatct ctgcattttc a
//
LOCUS       DAZGAN020000046        32675 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGAN020000046 DAZGAN020000000
VERSION     DAZGAN020000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.046"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            86..1375
                     /gene="hpaG"
                     /locus_tag="ACHKYO_RS12025"
                     /old_locus_tag="pgaptmp_002405"
     CDS             86..1375
                     /gene="hpaG"
                     /locus_tag="ACHKYO_RS12025"
                     /old_locus_tag="pgaptmp_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HGU4429242.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKIREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1372..2838
                     /gene="hpaE"
                     /locus_tag="ACHKYO_RS12030"
                     /old_locus_tag="pgaptmp_002406"
     CDS             1372..2838
                     /gene="hpaE"
                     /locus_tag="ACHKYO_RS12030"
                     /old_locus_tag="pgaptmp_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4429243.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2840..3691
                     /gene="hpaD"
                     /locus_tag="ACHKYO_RS12035"
                     /old_locus_tag="pgaptmp_002407"
     CDS             2840..3691
                     /gene="hpaD"
                     /locus_tag="ACHKYO_RS12035"
                     /old_locus_tag="pgaptmp_002407"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HGU4429244.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3701..4081
                     /locus_tag="ACHKYO_RS12040"
                     /old_locus_tag="pgaptmp_002408"
     CDS             3701..4081
                     /locus_tag="ACHKYO_RS12040"
                     /old_locus_tag="pgaptmp_002408"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HGU4429245.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4192..4995
                     /gene="hpaH"
                     /locus_tag="ACHKYO_RS12045"
                     /old_locus_tag="pgaptmp_002409"
     CDS             4192..4995
                     /gene="hpaH"
                     /locus_tag="ACHKYO_RS12045"
                     /old_locus_tag="pgaptmp_002409"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HGU4429246.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            5006..5794
                     /gene="hpaI"
                     /locus_tag="ACHKYO_RS12050"
                     /old_locus_tag="pgaptmp_002410"
     CDS             5006..5794
                     /gene="hpaI"
                     /locus_tag="ACHKYO_RS12050"
                     /old_locus_tag="pgaptmp_002410"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HGU4429247.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5917..7293
                     /gene="hpaX"
                     /locus_tag="ACHKYO_RS12055"
                     /old_locus_tag="pgaptmp_002411"
     CDS             5917..7293
                     /gene="hpaX"
                     /locus_tag="ACHKYO_RS12055"
                     /old_locus_tag="pgaptmp_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HGU4429248.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSLVSGYILSLDGVMALKGWQWLFLLESFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVATLLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7303..8193
                     /gene="hpaA"
                     /locus_tag="ACHKYO_RS12060"
                     /old_locus_tag="pgaptmp_002412"
     CDS             7303..8193
                     /gene="hpaA"
                     /locus_tag="ACHKYO_RS12060"
                     /old_locus_tag="pgaptmp_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HGU4429249.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8444..10006
                     /gene="hpaB"
                     /locus_tag="ACHKYO_RS12065"
                     /old_locus_tag="pgaptmp_002413"
     CDS             8444..10006
                     /gene="hpaB"
                     /locus_tag="ACHKYO_RS12065"
                     /old_locus_tag="pgaptmp_002413"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HGU4429250.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGSMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10024..10536
                     /locus_tag="ACHKYO_RS12070"
                     /old_locus_tag="pgaptmp_002414"
     CDS             10024..10536
                     /locus_tag="ACHKYO_RS12070"
                     /old_locus_tag="pgaptmp_002414"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HGU4429251.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10929..13079
                     /gene="btsT"
                     /locus_tag="ACHKYO_RS12075"
                     /old_locus_tag="pgaptmp_002415"
     CDS             10929..13079
                     /gene="btsT"
                     /locus_tag="ACHKYO_RS12075"
                     /old_locus_tag="pgaptmp_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HGU4429252.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLNVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13129..13332
                     /gene="yjiX"
                     /locus_tag="ACHKYO_RS12080"
                     /old_locus_tag="pgaptmp_002416"
     CDS             13129..13332
                     /gene="yjiX"
                     /locus_tag="ACHKYO_RS12080"
                     /old_locus_tag="pgaptmp_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU4429253.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13343..14299
                     /gene="yjiA"
                     /locus_tag="ACHKYO_RS12085"
                     /old_locus_tag="pgaptmp_002417"
     CDS             13343..14299
                     /gene="yjiA"
                     /locus_tag="ACHKYO_RS12085"
                     /old_locus_tag="pgaptmp_002417"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGU4429254.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(14377..16917)
                     /locus_tag="ACHKYO_RS12090"
                     /old_locus_tag="pgaptmp_002418"
     CDS             complement(14377..16917)
                     /locus_tag="ACHKYO_RS12090"
                     /old_locus_tag="pgaptmp_002418"
                     /EC_number="3.5.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008953 - penicillin amidase activity
                     [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HGU4429255.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVETLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(17269..17433)
                     /gene="yjiS"
                     /locus_tag="ACHKYO_RS12095"
                     /old_locus_tag="pgaptmp_002419"
     CDS             complement(17269..17433)
                     /gene="yjiS"
                     /locus_tag="ACHKYO_RS12095"
                     /old_locus_tag="pgaptmp_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HGU4429256.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            17610..19022
                     /gene="yjiR"
                     /locus_tag="ACHKYO_RS12100"
                     /old_locus_tag="pgaptmp_002420"
     CDS             17610..19022
                     /gene="yjiR"
                     /locus_tag="ACHKYO_RS12100"
                     /old_locus_tag="pgaptmp_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU4429257.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMRLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(19264..20208)
                     /locus_tag="ACHKYO_RS12105"
                     /old_locus_tag="pgaptmp_002421"
     CDS             complement(19264..20208)
                     /locus_tag="ACHKYO_RS12105"
                     /old_locus_tag="pgaptmp_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGU4429258.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            20675..21907
                     /gene="mdtM"
                     /locus_tag="ACHKYO_RS12110"
                     /old_locus_tag="pgaptmp_002422"
     CDS             20675..21907
                     /gene="mdtM"
                     /locus_tag="ACHKYO_RS12110"
                     /old_locus_tag="pgaptmp_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HGU4429259.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            21948..23228
                     /gene="yjiN"
                     /locus_tag="ACHKYO_RS12115"
                     /old_locus_tag="pgaptmp_002423"
     CDS             21948..23228
                     /gene="yjiN"
                     /locus_tag="ACHKYO_RS12115"
                     /old_locus_tag="pgaptmp_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HGU4429260.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPVLLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            23344..24495
                     /gene="yjiM"
                     /locus_tag="ACHKYO_RS12120"
                     /old_locus_tag="pgaptmp_002424"
     CDS             23344..24495
                     /gene="yjiM"
                     /locus_tag="ACHKYO_RS12120"
                     /old_locus_tag="pgaptmp_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HGU4429261.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            24505..25272
                     /gene="yjiL"
                     /locus_tag="ACHKYO_RS12125"
                     /old_locus_tag="pgaptmp_002425"
     CDS             24505..25272
                     /gene="yjiL"
                     /locus_tag="ACHKYO_RS12125"
                     /old_locus_tag="pgaptmp_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HGU4429262.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTHR"
     gene            25269..25526
                     /gene="ytiA"
                     /locus_tag="ACHKYO_RS12130"
                     /old_locus_tag="pgaptmp_002426"
     CDS             25269..25526
                     /gene="ytiA"
                     /locus_tag="ACHKYO_RS12130"
                     /old_locus_tag="pgaptmp_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HGU4429263.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTESIYCQQDGVWRCIAHYGVSPR"
     gene            25591..26451
                     /gene="yjiK"
                     /locus_tag="ACHKYO_RS12135"
                     /old_locus_tag="pgaptmp_002427"
     CDS             25591..26451
                     /gene="yjiK"
                     /locus_tag="ACHKYO_RS12135"
                     /old_locus_tag="pgaptmp_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGU4429264.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            26519..27697
                     /gene="yjiJ"
                     /locus_tag="ACHKYO_RS12140"
                     /old_locus_tag="pgaptmp_002428"
     CDS             26519..27697
                     /gene="yjiJ"
                     /locus_tag="ACHKYO_RS12140"
                     /old_locus_tag="pgaptmp_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4429265.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPLLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(27710..28264)
                     /gene="kptA"
                     /locus_tag="ACHKYO_RS12145"
                     /old_locus_tag="pgaptmp_002429"
     CDS             complement(27710..28264)
                     /gene="kptA"
                     /locus_tag="ACHKYO_RS12145"
                     /old_locus_tag="pgaptmp_002429"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HGU4429266.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            28514..29197
                     /gene="yjiH"
                     /locus_tag="ACHKYO_RS12150"
                     /old_locus_tag="pgaptmp_002430"
     CDS             28514..29197
                     /gene="yjiH"
                     /locus_tag="ACHKYO_RS12150"
                     /old_locus_tag="pgaptmp_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGU4429267.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            29194..29655
                     /gene="yjiG"
                     /locus_tag="ACHKYO_RS12155"
                     /old_locus_tag="pgaptmp_002431"
     CDS             29194..29655
                     /gene="yjiG"
                     /locus_tag="ACHKYO_RS12155"
                     /old_locus_tag="pgaptmp_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HGU4429268.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            29668..30840
                     /gene="iadA"
                     /locus_tag="ACHKYO_RS12160"
                     /old_locus_tag="pgaptmp_002432"
     CDS             29668..30840
                     /gene="iadA"
                     /locus_tag="ACHKYO_RS12160"
                     /old_locus_tag="pgaptmp_002432"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU4429269.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVNGTFETA"
     gene            30905..31816
                     /gene="hypT"
                     /locus_tag="ACHKYO_RS12165"
                     /old_locus_tag="pgaptmp_002433"
                     /gene_synonym="qseD"
     CDS             30905..31816
                     /gene="hypT"
                     /locus_tag="ACHKYO_RS12165"
                     /old_locus_tag="pgaptmp_002433"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HGU4429270.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(31809..32201)
                     /gene="iraD"
                     /locus_tag="ACHKYO_RS12170"
                     /old_locus_tag="pgaptmp_002434"
     CDS             complement(31809..32201)
                     /gene="iraD"
                     /locus_tag="ACHKYO_RS12170"
                     /old_locus_tag="pgaptmp_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HGU4429271.1"
                     /translation="MMRQSLQAVLPEISGNKTSSLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(32198..32281)
                     /gene="idlP"
                     /locus_tag="ACHKYO_RS12175"
                     /old_locus_tag="pgaptmp_002435"
     CDS             complement(32198..32281)
                     /gene="idlP"
                     /locus_tag="ACHKYO_RS12175"
                     /old_locus_tag="pgaptmp_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HGU4429272.1"
                     /translation="MENEHQYSGARCSGQTAYVAKRQECAK"
     gene            complement(32397..32540)
                     /gene="ytiD"
                     /locus_tag="ACHKYO_RS12180"
                     /old_locus_tag="pgaptmp_002436"
     CDS             complement(32397..32540)
                     /gene="ytiD"
                     /locus_tag="ACHKYO_RS12180"
                     /old_locus_tag="pgaptmp_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HGU4429273.1"
                     /translation="MADYAEINNFPPELSSGGDKYFHLHNYSEYSEYTSGFFLSLMIF
                     IKS"
BASE COUNT         7536 a         8605 c         8540 g         7994 t
ORIGIN      
        1 cttacttttg ccaaactacg caatcgggaa taaaagtaat cattaacata ttaatgatta
       61 aaatgatcga atccgaggag tggtaatgaa aggcactatc ttcgccgtag cgttgaacca
      121 tcgcagccaa cttgatgcat ggcaggaagc gttccagcaa tccccctaca aagccccgcc
      181 taaaactgcg gtctggttta ttaaaccgcg caatacggtg attggttgcg gtgaaccgat
      241 tccctttcca cagggtgaaa aggtactgag cggtgcgact gttgcgctga ttgtgggaaa
      301 aacggcgacg aaaatacgtg aagaagatgc agcagagtac atcgccggat atgcgctggc
      361 taacgatgtc agcctgccgg aagagagctt ttaccgcccg gcaatcaaag caaaatgtcg
      421 tgatggattc tgccccattg gcgaaaccgt ggctctcagc aatgtcgata atctgaccat
      481 ctataccgag atcaacgggc gtcctgccga tcactggaat accgccgatt tacaacgtaa
      541 cgccgcgcag ttgctgagcg ccctgagcga atttgccaca ctgaatccag gcgatgccat
      601 tctgctcggc acgccacagg cgcgcgtgga aatacagcca ggcgatcgcg ttcgtgttct
      661 cgcagaaggt ttcccgccgc tggaaaatcc ggtagtggac gaacgtgaag tgaccacgcg
      721 caagagcttc ccaacgcagc cacacccgca cggcacgctg tttgccctcg gcctgaacta
      781 cgccgaccac gccagcgaac tggaatttaa gccaccggaa gaaccgctgg tgttcctgaa
      841 agcgccaaat accctcactg gcgataacca gacctccgtg cgtccaaaca atattgaata
      901 catgcactat gaagcggagc tggtggtagt tattggcaaa caggcgcgta acgtcagcga
      961 agccgatgcc atggattatg tcgcgggcta caccgtgtgt aacgactacg ccattcgcga
     1021 ctatctggaa aactactacc gccctaacct gcgggtcaaa agccgcgacg gactgacgcc
     1081 gatgctttca accatcgtgc cgaaagaggc gatcccggac ccgcataatc tgacccttcg
     1141 caccttcgtc aacggcgagt tacgccagca aggcaccacc gccgatctga tcttcagcgt
     1201 gcccttcctg atcgcctact taagcgaatt tatgaccctg aatccgggcg acatgatcgc
     1261 caccggcaca ccaaaaggct tatctgacgt ggtgcctggc gatgaagtgg tggtggaagt
     1321 agaaggcgtg ggccgcctgg tgaaccgaat tgtgagtgag gaaacagcga aatgaaaaaa
     1381 gtaaatcatt ggatcaacgg taaaaatgtt gcaggtaacg actacttcca gactaccaat
     1441 ccggcaacgg gtgaagtgct ggcggatgtg gcctccggcg gtgaagcgga gatcaatcag
     1501 gcggtagcgg cagcgaaaga ggcgttcccg aaatgggcca atctgccgat gaaagagcgc
     1561 gcgcgcctga tgcgccgtct gggcgatctg atcgaccaga acgtgccgga gatcgccgcg
     1621 atggaaaccg cggacacggg cctgccgatc catcagacca aaaatgtgtt gatcccacgc
     1681 gcttctcaca actttgaatt tttcgcggaa gtctgccagc agatgaacgg caagacttat
     1741 ccggtcgacg acaagatgct caactacacg ctggtgcagc cggtaggcgt ttgtgcactg
     1801 gtgtcaccgt ggaacgtgcc gtttatgacc gccacctgga aggtcgcgcc gtgtctggcg
     1861 ctgggcaata ccgcggtact gaaaatgtcg gaactctccc cgctgaccgc tgaccgcttg
     1921 ggtgagctgg cgctggaagc cggtattccg gcaggcgtgc tgaacgtggt acagggctac
     1981 ggcgcaaccg caggggatgc gctggttcgt catcatgacg tacgtgccgt gtcgttcacc
     2041 ggcggtacgg ccaccgggcg caacatcatg aaaaacgccg ggctgaaaaa atactccatg
     2101 gaactgggcg gtaaatcgcc ggtgctgatt tttgaagatg ccgatattga acgcgcgctg
     2161 gacgccgccc tgttcaccat cttctcgatc aacggcgaac gctgcaccgc cggttcgcgc
     2221 atctttattc agcaaagcat ctacccggaa ttcgttaaac gctttgccga acgcgccaac
     2281 cgtctgcgcg tgggcgatcc gaacgatccg aatacccagg ttggcgcgct tattagccag
     2341 cagcactggg aaaaagtctc cggctatatc cgtctcggca ttgaagaagg cgcaaccctg
     2401 ctggcgggcg gcccggataa accgtccgac ctgcctgcgc acctgaaagg cggcaacttc
     2461 ctgcgcccaa ccgtgctggc agacgttgat aaccgtatgc gagtcgccca ggaagagatt
     2521 ttcgggccgg tcgcctgcct gctgccgttt aaagacgaag ccgaaggctt acgtctggca
     2581 aacgacgtgg agtacggcct cgcgtcgtac atctggacac aggatgtcag caaagtgtta
     2641 cgcctggcgc gtggcattga agctggcatg gtgttcgtca acacccagaa cgtgcgtgac
     2701 ctgcgccagc catttggcgg cgtaaaagcc tccggcaccg ggcgtgaagg cggtgagtac
     2761 agcttcgaag tgttcgcgga aatgaagaac gtctgcattt ccatgggcga ccatccaatt
     2821 ccgaaatggg gagtctgata tgggtaagtt agcgttagcc gccaagatca cgcacgtacc
     2881 gtcgatgtat ctctctgaac tgccagggaa aaaccacggt tgccgccagg gcgcgatcga
     2941 cgggcataaa gagatcagca agcgttgccg ggaaatgggc gtcgatacca ttatcgtttt
     3001 cgatacccac tggctggtca acagtgctta tcacatcaac tgtgcagacc attttgaagg
     3061 cgtctacacc agcaacgagc tgccgcattt tatccgcgat atgacctaca actacgaggg
     3121 caacccggag ttggggcagc ttattgccga tgaagcctta aagctcggcg tgcgggcaaa
     3181 agcgcacaac attcccagcc tgaaactgga atacggcacg ctggtgccaa tgcgctacat
     3241 gaatgaagac aagcacttca aagtggtttc catttcagct ttctgcactg ttcacgattt
     3301 tgccgacagc cgcaagctgg gcgaggcgat tctgaaagcg atcgaacagt acgacggcac
     3361 cgtggcggtc cttgccagcg gttcgttatc gcaccgcttt attgacgatc agcgagcgga
     3421 agaagggatg aacagctaca cccgcgagtt cgaccgccag atggacgagc gtgtggtgaa
     3481 gctgtggcgc gaaggccagt tcaaagagtt ctgcaatatg ctgccggagt acgccgacta
     3541 ctgctacggc gaaggcaata tgcacgacac ggtgatgctg ctggggatgc tcggctggga
     3601 taaatacgac ggcaaggtgg agtttatcac cgagctattc ccaagctctg gcaccggtca
     3661 ggttaacgct gttttcccgc ttcccgcgta aggacgtttt atgccgcact ttatcgttga
     3721 atgcagtgac aacatccgcg aagaagccga cctgccgggg ttgttcgcca aagtgaatcc
     3781 gacgctggca gccacgggta tttttccgct ggcgggtatt cgcagccgcg tgcattgggt
     3841 cgatacctgg cagatggccg acgggcagcg tgattatgcc ttcgtgcata tgacgttgaa
     3901 aatcggcgca ggtcgcagcc tggaaagccg tcagcaggcg ggtgaaatgc tgtttgaact
     3961 gattaaaacg cacttcgccg ccctgatgga gagccgcctg ctggcgttgt cgtttgagat
     4021 tgaagagctg catccgacgc tgaattttaa acaaaacaac gtgcacgcat tgtttaagta
     4081 gcgcgcagat tgcccggtgg cgctgcgctt accgggccta caaaacccca aaccgtacac
     4141 cgtaggccgg ataaggcgca gccgcatccg gcaatgccac aggatatcgc tatgttcgat
     4201 aaacataccc acaccctgat cgcccagcgt ctggatcagg cagaaaaaca gcgcgaacag
     4261 atccgcgcga tctcgctgga ttacccggag atcaccatcg aagacgctta cgcggtgcag
     4321 cgtgaatggg ttcgcctgaa aatagccgaa ggtcgcacgc tgaaaggcca caaaatcggc
     4381 ctgacctcga aagcgatgca ggccagctca caaatcagcg agccggatta cggtgccctg
     4441 ctggatgaca tgttcttcca cgatggcagc gatatcccga ccgatcgctt tatcgtgccg
     4501 cgcattgaag tggagctggc tttcgtgctg gcaaaaccgc tgcgtgggcc aaactgcacg
     4561 ctgttcgacg tctacaacgc cacggactac gtgatcccgg cgctggagct gatcgacgct
     4621 cgctgccaca acatcgatcc ggaaacccag cgcccgcgca aagtgttcga caccatttct
     4681 gataacgccg ccaacgccgg ggtgatcctc ggtggtcgtc ccattaagcc cgatgagttg
     4741 gatctgcgtt ggatctccgc cctgatgtat cgcaatggcg tgattgaaga aaccggcgtc
     4801 gccgctggtg tgctaaatca tccggcaaac ggcgtggcct ggctggcgaa caaactcgca
     4861 ccgtatgacg tacaactgga agccgggcaa atcattctcg gcggttcgtt cacccgcccg
     4921 gttccggcgc gtaagggcga caccttccac gtcgattacg gcaacatggg ctccattagc
     4981 tgccgctttg tttaaggaga gaacgatgga aaacagtttt aaagcggcgc tgaaagcagg
     5041 ccgcccgcag atcggattat ggctggggct gagtagcagc tacagcgcag agttactggc
     5101 cggagcagga ttcgactggt tgttgatcga cggtgagcac gcgccgaata acgtgcaaac
     5161 cgtgctcacc cagctacagg cgattgcgcc ctaccccagc cagccggtag tacgtccgtc
     5221 gtggaacgat ccggtgcaaa tcaaacaact gctggacgtc ggcacacaaa ccttgctggt
     5281 gccgatggta caaaacgctg acgaagcccg tgaagcggta cgcgccaccc gttatccccc
     5341 cgccggtatt cgcggtgtgg gcagtgcgct ggcccgcgcc tcgcgctgga atcgtattcc
     5401 tgattacctg caaaaagcca acgatcaaat gtgcgtgctg gtgcagatcg aaacgcgtga
     5461 ggcaatgaag aacttaccgc agattctgga cgtggaaggc gtcgacggcg tgtttatcgg
     5521 cccggcggat ctgagcgccg atatgggtta tgccggtaat ccgcagcacc cggaagtaca
     5581 ggccgccatt gagcaggcga tcgtgcagat ccgtgaatcg ggcaaagcgc cggggatcct
     5641 gatcgccaat gagcaactgg caaaacgcta tctggaactg ggcgcgctgt ttgtcgccgt
     5701 cggcgttgac accaccctgc tcgcccgcgc cgctgaagcg ctggcagcac ggtttggtgc
     5761 gcaggctacc gccgtgaagc ccggcgtgta ttaatgccgg atgcgctgcg cttatccggc
     5821 ctacactcgc accaaatcgt aggccggata aggcgttacg ccgcatccag caaaaacctt
     5881 gtcgtaccct acaaaaatcc cattagagga agaaaaatga gcgacacctc acctgccata
     5941 ccggagagta tcgatccggc gaatcagcat aaagcgctga ctgccggaca acaggcggtt
     6001 attaagaagc tatttcgccg cctgatcgtc tttctgttcg tgctgtttat cttctcgttc
     6061 cttgatcgca tcaacatcgg ctttgccgga ctcacgatgg gacgcgacct cggtctgagc
     6121 gccaccatgt ttggcctcgc taccaccctg ttctacgccg cttatgtcat cttcggcatt
     6181 cccagcaaca ttatgctgag tattgtcggt gcgcggcgct ggatcgccac catcatggtg
     6241 ctttggggca tcgcctctac tgccaccatg tttgccactg gccccaccag cttgtacgta
     6301 ctgcgtatac tggttggcat taccgaagcc ggctttctgc ctggcattct gctgtattta
     6361 accttctggt ttccagccta tttccgcgcc cgtgccaacg ccttgtttat ggttgcaatg
     6421 ccggtaacga cagcgttggg atcgctcgtt tccggctaca ttttgtcgct ggatggcgta
     6481 atggcattaa aaggctggca gtggctgttt ttgctggaaa gcttcccgtc ggtattactc
     6541 ggcgtcatgg tgtggttctg gcttgatgac tcaccggaca aagctaagtg gctgacgaaa
     6601 gaagacaaaa aatgcctgca agagatgatg gataacgatc gtctgacgct ggttcagcca
     6661 gagggagcca tcagccacca cgccatgcaa caacgcagca tgtggcggga gatcttcact
     6721 ccggtggtga tgatgtatac cctggcgtat ttctgcctga ccaacacact tagtgcgatc
     6781 agcatctgga caccgcagat cctgcaaagc tttaatcagg gcagcagtaa tatcaccatc
     6841 ggcctgctgg ccgccgtacc gcagatttgt accattctcg ggatgatcta ctggagccgt
     6901 cactcagatc gccgccagga acgaaggcat cacaccgccc ttccttattt gttcgctgcc
     6961 gcgggttggt tactggcttc ggcaactgat cacaacatga tccagatgct ggggatcatt
     7021 atggcttcga ccggatcatt cagcgcaatg gcgattttct ggacaacacc ggatcagtcc
     7081 atcagcctgc gggcacgagc gatcggtatt gcggtgatca acgccactgg caacattggt
     7141 tcagcgttaa gtccgtttat gatcggctgg ttgaaagatc tgaccggcag ctttaacagt
     7201 ggattgtggt ttgttgccac gctgctggtg attggtgcgg ggattatctg ggcgattcca
     7261 atgcagtcct cccgtccgcg agcgaccccg taaggaacga cgatgtgtga ccgtcagatt
     7321 gccaatattg atatcagcaa agagtacgat gaaagcctgg gcacggacga tgtgcattat
     7381 cagtcgttcg cccgcatggc ggcatttttt ggccgccata tgctgccaca tcgccacgaa
     7441 cagtactttc agatgcattt cctcaatagc ggacagattg agctacagct tgacgatcat
     7501 cgctactcgg tggaagcgcc cctgtttgtc ctgacgccac cgtcagtacc tcatgcgttt
     7561 attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct gatctggccc
     7621 ctgctggaag ttctttatcc gggcactcgg gaaaccttcg gcctgccggg gatttgcctg
     7681 tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca actgatagag
     7741 cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgttact ggcacaggca
     7801 gtgttcaccc tactgctgcg taacgcaaaa ctcgacgacc atgccgccag cgggatgcgc
     7861 ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt tcatcagcac
     7921 tggacagtac cggattacgc taacgaactg catatcaccg aatcacgact cacggacatc
     7981 tgccgtcgct ttgccaaccg tccgccaaaa cggttgattt tcgacaggca gctgcgagaa
     8041 gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg gcaactcggt
     8101 tttaaggatc cggcttattt tgcgcgcttt tttaatcgct tagtcggttg ctcgcccagt
     8161 gcttatcgcg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa cgggaaacca
     8221 ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta acgtcaaaaa
     8281 tcgctaaaag cgacctcgat cacaaaaccg tacccgacct gaaaagtacc agccattgtc
     8341 ccaaaagtct cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa aaacaacaca
     8401 caaaatcaac aattgtattc cgattaatac tgtagaggtc gacatgaaac cagaagattt
     8461 ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc tgcaggatgg
     8521 tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc cggcatttcg
     8581 taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg agatgcagga
     8641 ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat tcttccgcgt
     8701 ggcgaaaagt gccgacgacc tgcgccagca acgcgacgcc atcgctgagt ggtcacgcct
     8761 gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt gcgcactggg
     8821 cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt acacccgtat
     8881 tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg atcgtcattt
     8941 gccgaccgat aaagtaaaag acgtttacat caagctggaa aaagagactg acgccgggat
     9001 tatcgtcagc ggtgcgaaag tggttgccac caactcggcg ctgactcact acaacatgat
     9061 tggcttcggc tcggcacaag tgatgggcga aaacccggac ttcgcactga tgttcgttgc
     9121 gccaatggat gccgatggcg tgaaattaat ctcccgcgcc tcttatgaga tggtcgcggg
     9181 tgctaccggc tcgccatacg actacccgct ctccagccgc ttcgatgaga acgatgcgat
     9241 tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc gcgatttcga
     9301 tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc tgcaagcctg
     9361 tgtgcgcctg gcagtgaaat tagacttcat tacagcactg ctgaaaaaat ctctcgaatg
     9421 taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg tggcgtggcg
     9481 caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt gggtcaacgg
     9541 ggcttattta ccggatcatg ccgcactgca aacctatcgc gtactggcac caatggccta
     9601 cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct atctcccttc
     9661 cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt atgtgcgcgg
     9721 ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt gggacgccat
     9781 tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg gtagccagga
     9841 tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcagtatgg acaagatgat
     9901 ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg tgccgcactt
     9961 gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca gcaggaggtt
    10021 aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag cctgtcggca
    10081 gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac ggcaacggcc
    10141 gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc caacagtgcg
    10201 atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa ccatgagcag
    10261 gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga gcgttttagc
    10321 ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc gctggccagt
    10381 cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta tctggtggag
    10441 attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa acgccgtttc
    10501 catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc acgcattcgt
    10561 cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata attcttgccc
    10621 aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca gttatttacc
    10681 ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca atttacctcc
    10741 tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta ctctatgaat
    10801 tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac agtaagtgac
    10861 aggggtgaac gaacgcaact gccgcacctg taagctaaaa gacgacgagt aaaatgccag
    10921 gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt ctcggaatca
    10981 tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc agcgccctgt
    11041 ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac agtctgtaca
    11101 tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt attaacaacg
    11161 acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac ttcgccgcta
    11221 tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc tacctgcctg
    11281 gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac tttatggtgc
    11341 tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa gaagagatgg
    11401 ggccagttcc ggggactatc gcgctgtttg gctgtttctt aatcatgatc atcatcctcg
    11461 ccgttctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt gtcttcaccg
    11521 tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt atccgtccgg
    11581 ggcgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct atctacttcg
    11641 gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa gacaccacca
    11701 ttaccttcgc gctgattggc tatgcgtttg tttccgcact gctgccggtg tggctgatcc
    11761 tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc ggcctggcgc
    11821 tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag tacattgacg
    11881 gtactggccc gctgtggaaa ggcgctctgt tcccgttcct gttcatcacc atcgcctgtg
    11941 gtgcggtatc tggcttccac gcgctgatct cttccggtac gacgccaaaa ctgctggcta
    12001 acgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc ttcgtggcga
    12061 ttatggcgct ggttgcagcg tccatcatcg aaccgggtct ttacttcgcg atgaataccc
    12121 cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc gagaacgcgc
    12181 cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc gtcagctcct
    12241 ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt ggtgagcctt
    12301 ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct cacgtgttcc
    12361 acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt ctgttcgaag
    12421 ccctgttcat cctgaccgcg ctggatgcgg gtactcgttc tggccgcttt atgctgcaag
    12481 acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt gccggtatca
    12541 tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc gtggtcgatc
    12601 cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ttccaaccag atgctggcag
    12661 ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc caatacatct
    12721 gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg ctgggtctga
    12781 aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc cagtacaaag
    12841 agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg aaccacatcg
    12901 ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg gtgtacagca
    12961 tcatcttcta cggtttcaaa acctggctga atgtgcgtaa cagcgacaaa cgtactgaca
    13021 aagaaacacc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg caccactaac
    13081 cgtatttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat gtttggtaac
    13141 ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg cattccagac
    13201 tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta catgagctat
    13261 gaagaattct tccgcgaacg ccagaacgca cgctacggcg gcgatggtaa aggcggtatg
    13321 cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca ccggtttttt
    13381 aggcgcgggc aaaaccaccc tgctgcgcca tattcttaat gaacaacacg gctacaagat
    13441 tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaattga ttggtgatcg
    13501 cgcaacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct ccaacgagct
    13561 ggaggacgcg ctactcgacc tgctggataa tctcgacaag ggcaatattc agttcgatcg
    13621 tctggtcatt gaatgcaccg gcatggccga tcctggcccg attattcaaa cctttttctc
    13681 ccatgagatt ttatgccagc gctacctgct ggacggcgtg attgcgctgg tggatgcggt
    13741 acatgccgat gagcagatga accagttcac catcgcccag tcccaggtgg gctacgccga
    13801 ccgtattctg ctgactaaaa ccgacgtcgc aggcgaagca gaaaaactgc gtgaacgcct
    13861 ggcgcgcatc aacgcccgtg caccggtcta caccgtcacc cacggcgaca tcgacctggg
    13921 cctgctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca ccaaaccgcg
    13981 tttccacttt atcgcggata aacaaaacga tatttcgtcg attgtggtgg aactggatta
    14041 cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg aatcggcgga
    14101 taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc gcctgctgtt
    14161 ccagggcgtc cagcgcctct acagcgccga ctgggacagg ccatggggcg atgaaacacc
    14221 gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc gggcggcgtt
    14281 tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact ttatcaccct
    14341 catattcctc cagaatatga gggctttcgg gcttaattat ctctgaacgt gcaacacttc
    14401 ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact tacggccaaa
    14461 attttcgtac attttcagct gatcttcata gtgcttatca actgttccat cgggagcaat
    14521 aaacccactc tgaccgggtg cgaccacatc ccaggcaagc acaggacgat cgcttgtcgt
    14581 tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg cctgatgacg
    14641 cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcccgga acgttaaggc
    14701 cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa gagtctccca
    14761 ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa acagatcaac
    14821 cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt ttgctccaac
    14881 acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac tggcgctgta
    14941 ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca tactggtcag
    15001 ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat cattaagcaa
    15061 attgatgcca tcccaacgtg ttaatgtttc taccaactga cgacgcggat cgctctgtgt
    15121 caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa gatcctgacg
    15181 actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct tttgctcaag
    15241 cagtcggtcg atctccgtaa cgcgatctgc accaccccac aaaaaggcaa acagatctga
    15301 agcgggataa tctttttggg gagaattgtt ccagttagca atatatcccg actgggggtt
    15361 atacacctta gggttcattt caaaaggcaa tagccctttc cagtcccatt ttcccgtacc
    15421 aggaacgggt aatcgcggat catggcctga ttgacgatct ggataagcac cagtatgaac
    15481 ataaccaata ttgccgttta catcagcata gtaccagttg atggtcagtg cttgtttcgc
    15541 tgcctgctgt gtccactcct gccaattttt ggccttcatc tgatgagtcc aggccagcaa
    15601 agacgccacc tctttaccat cccatgcgcg ggatttagcg taagccgttt gtgtcgtctg
    15661 gtcagtttgg agaatgttgc catgcaccgt acgccagaca gtaaaggtct ctgcctgacc
    15721 atttttcacc gtaatggttt cctcacggct taacattttc acccacttac cattatgcaa
    15781 gtagtagcct ggtttctctg ccgacagccg ttcagcaaaa atatcgacat catcgccgaa
    15841 acctgccgtt gatccccagg aaatcacacc attatgacca aaaaccagcc caggataggc
    15901 aaatggtgta ttgccagtga catcataacc agcaccgtgc agaccaatac cataagtata
    15961 cgcaggcgca taccagccaa actgcggacc atttaccatg attgctttcg catcctgggc
    16021 tttgcttttg ccgatcaccc acatattgct ggtcgttgga taccccgcca gaccattggc
    16081 accaccctgt gcaaattgtg cagcaatagt ttcccggttc ttccctgctg ttaacgccag
    16141 cagtgcgcca tccgcccctt ttgctggtcg gtcaagcatt ggtgcaggta aatcgtagcg
    16201 tggcaacaga gctgctgttt gcgagttttg ctgattaaat ttaagtgggt agttactctc
    16261 ttgtacggca atagtggttg gcgctgatgg gtttaccagc catttcaact gattaaatac
    16321 cgccatgcct tgtgatacac catatttatc ttttaaagcc gttagcagtg ccagattatc
    16381 aatttcgcta gtgctatcag agaagcggtt tgccatggtg cccacaaata tcatcgcgac
    16441 atcaaacggt tcccagcgct taggagtaaa gccaaatgta ttaaactgtt ttggtaagag
    16501 cgtctctgga ttggtattta ccttatcaat ccaggcattc attccatcag cgtagccttg
    16561 cagaatggac atatcctctg gggaaagggc agcaatttgc gcccggatag catccggcca
    16621 gtagttacga cggatatctt tatcaaattt cacaaaatct ttgccaagca cttccgcgac
    16681 agtcccttga gtactgcgac gtgccatttc catctgaaaa aggcgatctt gtgctactac
    16741 atagccatag ccataaaata ggtgccatgt atcattggca taaatatgcg gcatgccgta
    16801 ttcatcgcga acaatcttta tctcacttga cgactgctca gccagtgcag gtaagctcca
    16861 ataatacatc agggaagcag taacacagtt cacgatcata cgatttctat ttttcattgt
    16921 atcctctggc aggtgtataa ttagcgaatt gatactagca acgaagcttc ggttatctat
    16981 ttgcagagaa ttgtttcatt atgaactgtg agcgcgataa ctaatttttg atacaaaaat
    17041 ttcattaatg tgatctaaaa cgaatcgctt tattcaccgc tgatgatttt gttctattcc
    17101 tttttgtgac agatcccccg gacgcataga tgatgacccc cagagagaca gctcaggtca
    17161 ttatctgtat gtaaatcaga aaaatatgtt gtaaacgact tttatggttg gattttgtag
    17221 caattttgaa tgcagacagg cagcatttgc caacacgccg cccatagttc actccacatc
    17281 ctccctgcgt aacccaatat ccctcaaccg ttcatcactc atctgctgta acactcgtcg
    17341 ggtctgcatt tgccgccgcc aacgcctcac cgcatgccag agttgcacaa gtctgataaa
    17401 cggcgttttg gctttgtttt cgtgaaattc catctgctgc ctcctcatct ttcagaggct
    17461 tcatcatccg ccttctggca gttcacaata cagatgcaaa tattagtttt atttaacata
    17521 cagacaggtt aaaacagcat ctgcatccgg ttttttagcc tcatctgtac cggtttttct
    17581 ttctgtatgg gccaaataag gaatacagca tgacgcgtta tcaacatctg gcgactctac
    17641 ttgccgagcg gattgagcaa gggctgtatc gtcacgggga gaaattgccg tcggtgcgca
    17701 gcttaagtca ggagcacggc gtcagcatca gcaccgtgca acaggcgtat cagacgctgg
    17761 aaacgatgag gctcatcact ccgcagccgc gttcgggtta ttttgtcgca caacgtaaag
    17821 cccagccgcc tgtaccgccg atgacgcgtc cggtgcagcg cccggtggaa attacccagt
    17881 gggatcaggt gctggatatg ctggaggcgc atagcgacaa ttccattgtt ccgttaagca
    17941 aaagcacgcc ggatgtcgaa acgcccagcc tgaaaccgct gtggcgtgag ctaagccggg
    18001 tggtgcagca taatctgcaa accgttctcg gttatgactt gttagccggt cagcgagtgt
    18061 tgcgggagca gattgcccgc ctgatgctcg acagcggctc ggtggtcacc gccgatgaca
    18121 tcatcatcac cagcggctgc cataactcga tgtcgctggc gttaatggcg gtgtgtaaac
    18181 cgggcgatat tgtcgcggtc gaatctccct gttattacgg ttcgatgcag atgctgcgag
    18241 gcatgggcgt gaaagtgatt gaaatcccaa ccgatccaga aactggcatc agcgttgaag
    18301 cactggaact ggcgctggaa cagtggccga ttaaaggcat cattctggtg ccaaactgta
    18361 ataatccgct gggatttatt atgccggacg cacgcaaacg ggccgttctc tctctcgctc
    18421 agcgtcatga tattgtgatt tttgaagatg atgtctacgg cgaactggca acggagtatc
    18481 cgcgcccgcg gaccatccat tcatgggata tcgacgggcg agtgctgttg tgcagctcgt
    18541 tcagtaaaag tattgctcca ggcctgcgcg tgggttgggt cgcaccgggg cgttatcacg
    18601 ataaactgat gcatatgaaa tacgccatca gcagctttaa tgtgccgtcc acgcaaatgg
    18661 cggcggcaac gtttgtgttg gaaggccact atcatcgcca tatccggcgg atgcggcaga
    18721 cttatcagcg caatctggcg ctttatacct gctggatacg ggaatatttt ccctgcgaaa
    18781 tctgtattac gcgcccgaaa ggcggatttt tactgtggat agaattgcct gaacaggtcg
    18841 atatggtctg cgttgcgcgg cagctgtgcc gcatgaatat ccaggtggca gcaggctcga
    18901 ttttctcggc ttccggcaaa taccgtaatt gtctacgcat caactgcgct ttgccgctca
    18961 gcgaaaccta tcgcgaagca ctaaagcaaa ttggcgaggc cgtgtatcgg gcaatggaat
    19021 aaattcgaat tagtggcgaa gcacctgatt tcctctcacg aatcgcctgt aactcgttgt
    19081 atctttgcag ggtcatcgcc acgttccagc atggcttagg caaagtgtgc ttcgttcata
    19141 ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccgc gcaaattcaa tagattgcag
    19201 aggaaatgta ggtctgataa gcgtagcgca tcaggcagct ttgcgtttgt catccgtctc
    19261 tgattagtat tcgatccatg gatcgcgctt gatttcaggc aagggctgct ctaatgcctg
    19321 catttgctct gtcgaaagtc cggtaatctt ttgtatccat tcaggatctg ccccattctg
    19381 caacaacaat ctaaggaggc gttgttcccc ttcttctttc cccttttcca tccccaacag
    19441 ccatccatca ttataaattc gctctgcaat cgtcattatt cgctccctgt gttgtggcat
    19501 tcgacgggta agttcgctga taaacgcctt aaaacgcgct tcatcgccag tcagtaaaat
    19561 gtaatttaac agcgcagtta tctggctgtc attagcacac tctgtaacca gtaatactac
    19621 cagttggtca ataagcccca tcagatcgcg ctggcgaata tgcttttgga tcaactccaa
    19681 cagggcgact ctgcgatgct gcacaatctc gtcgtctggc acgacagtga catccaccag
    19741 cgggaacgct gcgctataaa gtttccgtgc ggtagtcggg gcggcaaatt cgtccagcca
    19801 gcacagggac caggggtaag gactacggct accgtgataa aacagcatcg ggataaccag
    19861 cggtagcggc cggcgtttat catgctcgat atggcgctgc atcaccgcca tggaatatcg
    19921 catcaggcga aacgccatat gaatatcctc gcggctctga tgttcaatca ctacgtaaat
    19981 ataaccatca ccttcacggg tctttaccga ccacagaata tcggagtgta gcgcccgcaa
    20041 tttttcatcg acaaagctgg cggattccag ttttaagctg tcgagatcgc acagttcacg
    20101 taaatctttg ggtaagtgga tctccataaa atcccgcgcg gtgtcagggt gagtgagaaa
    20161 ggatttaaat aatgcgtcat gtggcgtgct ggtcgtgaag tttgtcatgg cgatccgtca
    20221 cctgtagata agatggcccg actttagcga ctaacaaaac cattctcacc cggcaatttc
    20281 tcgttccccg aggtgctttc caggctattt ttgttcgtct gcaaacgata catttttccg
    20341 gaaacaggct cgcgttttac atttcctgta cccggcagat attccccttt ccaccgcgct
    20401 cttttgatta accgcaaatt ttaattacac ttaaggtgta tattttctat gcaacccatc
    20461 aattaaagag gtgtaatgtg ctgatgacta tttgtaatcg ttatacattc tgacctggag
    20521 tcagaaggta tttctctgtc tgtgtgttca caggcagtgt ggttgattac atgaaattca
    20581 gtacatttgt ccttctcgcc tgcctttcgt cattaccgac ggtattgaat ttcgttttcc
    20641 ccgttggggt tctccggaca aggagttgtt tgttatgcca cgtttttttg cccgccatgc
    20701 cgccacgctg tttttcccga tggcgttgat tttgtatgac tttgccgcgt acctgtcgac
    20761 ggatctgatc cagccgggga tcattaatgt ggtccgtgat tttaatgccg atgtcagtct
    20821 ggccccggca gccgtcagtc tctatctcgc gggcggtatg gcgttacagt ggctgctggg
    20881 gccgctttcc gacaggattg gccgcaggcc ggtgctgatt accggggcgc taatatttac
    20941 ccttgcctgc gctgcgacaa tgttcacaac gtctatgaca cagtttctta tcgcgcgtgc
    21001 aattcagggc accagtatct gttttattgc caccgttggt tatgtcacgg tgcaggaggc
    21061 gttcggccag acaaaaggga tcaagttgat ggcgattatc acctccatcg tactgattgc
    21121 gccgattatt ggcccgcttt ccggcgcagc tctgatgcac tttgtgcact ggaaagtcct
    21181 ttttgccatc attgcagtta tgggttttat ctcatttgtt ggcttactgc tggcgatgcc
    21241 agagacggtg aagcgcggcg cggttccatt tagcgccaaa agcgtctttc gcgattttcg
    21301 taatgtcttt tgcaatcggc tgttcctctt tggcgcagca accatctctt taagctatat
    21361 cccaatgatg agttgggtgg ctgtctcgcc ggtgatcctg atcgacgcgg gcggcttaac
    21421 aacttcgcag ttcgcctgga cacaggttcc ggtgttcggc gcggtgattg tcgcgaatgc
    21481 catcgtggcg cgtttcgtta aagatccgac cgaaccgcgc tttatctggc gtgctgtacc
    21541 cattcaactg gtcggcctcg cgctgttgat tatcggcaat ctgctgtcac cgcacgtctg
    21601 gctgtggtcg gtgctgggca ccagtttgta tgctttcggg attggtttga tcttcccgac
    21661 cttattccgc tttacgcttt tttccaataa cttaccgaaa gggaccgtct ccgcatcgct
    21721 gaatatggtg atcctgatgg tgatgtctgt ctcggtcgaa atcggccgct ggctgtggtt
    21781 taacggcggt cgcttgccgt ttcatctgtt agccgtcgtg gcgggcgtta tcgtcgtttt
    21841 caccctggca ggattgctca atcgcgtgcg ccaacatcag gctgccgagc tggcggagga
    21901 gcggtgattt ttgcgcgatc ctgccgtcag gctctattct taacgttatg aataaactca
    21961 ttgaactcag acgcgccaaa atgttggcgc tctctttact gcttatcgcc gctgctacct
    22021 ttgtcgttac gctgtttttg ccgcccaatt tttgggtgag cggcgtgaag gcgattgctg
    22081 aagcggcgat ggtcggcgcg ctggcggact ggtttgcggt ggtggcgctg tttcgccgcg
    22141 tgccgattcc gatcatttct cgtcatacgg cgattatccc gcgtaataaa gaccggattg
    22201 gcgaaaatct cggccagttc gtgcaggaaa aatttctcga tacccagtcg ctggtggcat
    22261 tgattcgacg ccacgaaccg gtgttgttga ttggcaactg gtttagtcag ccagaaaacg
    22321 cccgccgcgt tggtcagcat ctgttgcaga tcatgagcgg ttttcttgaa ctgaccgatg
    22381 atgcgcgtat tcagcgcctg cttaagcgcg cggtccatcg ggcgattgat aaggtcgatc
    22441 tttccggcac cagtgcgttg atgctggaga gtatgaccaa aaacgatcgt catcaggtgc
    22501 tactggatac gctgatcgca cagttgatcg cccttctcca gcgcgataaa tcgcgcaagt
    22561 ttattgccca gcaaattgtt cgctggctgg agagtgagca tccactgaaa gccaaaattc
    22621 tccccaccga atggttgggc gaacatagcg cggagttggt ttctgacgcg gtgaattctt
    22681 tgcttgatga tatcagccgt gatcgtgcgc atcagatccg tcatgcgttt gatcgcgcca
    22741 cttttgccct gatcgacaag ttgaaaaacg atccggaaat ggcagcgcga gccgatgccg
    22801 taaaaagtta tctgaaagaa gatgaagctt ttaaccgcta tctcagtgaa ttgtgggggg
    22861 atttacggga gtggctgaaa gcggatatca acagtgaaga ttctcgtgtg aaagaacgta
    22921 tcgcgcgggc gggtcaatgg tttggcgaaa cgttaattgc cgatgatgcc ttgcgggcgt
    22981 cgttaaatgg tcacctggaa caagccgcgc accgcgtcgc gcctgagttt tccgcattcc
    23041 tgacgcgcca catcagcgat acagtaaaaa gctgggatgc gcgggatatg tcgcggcaaa
    23101 tcgagttaaa tatcggcaaa gatctgcagt ttatccgtgt caacggtacg ctggttggcg
    23161 gttgtattgg gctaatttta tatttgttgt cgcagctccc ggccttgttc cccctcagca
    23221 atttttagaa atccatgaaa aactaagtgg ctaaccgcga gagagatcaa ataaaccaca
    23281 ctaaaagaat ggtaataatg tggcctcttt tcattatctc ccgtggtacg gggaaggaaa
    23341 atcatgtcac ttatcaccga tctacccgcc attttcgatc agttctctga agctcgccag
    23401 aaaggctttc tcaccgtcat ggatctcaag gagcgcggca ttccgctggt tggcacttac
    23461 tgcaccttta tgccgcaaga gatcccgatg gcagccggtg cggttgtggt ttcgctctgt
    23521 tctacctctg atgaaaccat tgaagaagcg gagaaagatc tgccgcgcaa cctctgcccg
    23581 ctgattaaaa gcagctacgg ctttggcaaa accgataaat gcccctactt ctacttttcg
    23641 gatctggtgg tcggtgaaac cacctgcgac ggcaaaaaga aaatgtatga atacatggcg
    23701 gagtttaagc ccgttcatgt gatgcagttg ccgaacagcg ttaaggacga tgcctcgcgt
    23761 gcgttatgga aagccgagat gctgcgcttg caaaaaacgg tagaagaacg ttttgggcac
    23821 gagattagcg aagatgctct gcgcgatgcc attgcgctga aaaaccgcga acgtcgcgca
    23881 ctggctaatt tttatcatct tgggcagtta aatcctccgg cgcttagcgg cagcgacatt
    23941 ctgaaagtgg tctacggcgc gaccttccgg ttcgataaag aggcgttgat caatgaactg
    24001 gacgcgatga cagcccgcgt tcgtcagcag tgggaagaag gccagcggct ggccctgcgt
    24061 ccacgcattt taatcaccgg ctgcccgatt ggcggcgcag cagaaaaagt ggtgcgcgcg
    24121 attgaagaga atggcggctg ggttgtcggt tatgaaaact gcaccggggc gaaagcgacc
    24181 gagcaatgcg tggcagaaac gggcgatgtc tacgacgcac tggcggataa atatctggcg
    24241 attggctgct cctgtgtttc gccgaacgat cagcgcctgc aaatgctcag ccagatggtg
    24301 gaagaatatc aggtcgatgg cgtagttgat gtgattttgc aggcgtgcca tacctacgcg
    24361 gtggaatcgc tggcgattaa acgtcatgtg cgtcagcagc acaacattcc ttatatcgct
    24421 attgaaacag actactccac ctcagatgtc gggcagctca gtacccgtgt cgcggccttt
    24481 attgagatgc tgtaaggagt ggcagtgaca tattcgattg gcattgattc tggttctacc
    24541 gccaccaaag ggatcttact ggcagacggc gtgattacgc gccgtttcct cgttccaaca
    24601 ccctttcgtc cggcaacagc aattactgaa gcctgggaaa ctctgcgcga agggttagag
    24661 acaacgccgt ttctgacgct caccggctat ggacggcaac tggtggattt tgccgataaa
    24721 caagtgacgg aaatctcctg tcatgggctg ggcgcacggt ttcttgcgcc agcaacgcgc
    24781 gcggtaatcg acatcggtgg tcaggacagc aaagtgattc agcttgatga tgacggtaac
    24841 ctgtgcgatt tcctgatgaa tgacaaatgc gcggcgggca ccgggcgttt cctggaggtg
    24901 atctcgcgca cgcttggcac cagcgtcgag caactcgaca gcattaccga aaatgtcacg
    24961 ccgcacgcca tcacgagtat gtgcacagtg tttgccgaat cagaagtgat cagcctgcgc
    25021 tcagcgggcg tcgcgccaga agcgattctc gccggagtga ttaacgcgat ggcgcggagg
    25081 agtgccaatt tcattgctcg tctctcctgt gaagcgccga ttctgtttac tggtggcgtt
    25141 agtcattgcc agacgtttgc ccggatgctg gaatctcatc tgggaatgcc ggtaaatacc
    25201 catcctgatg cgcaatttgc tggcgcaatt ggcgcggcgg taattggtca acgagtgagg
    25261 acacaccgat gaaagagttt ttatttttat ttcactccac ggtcggcgtc atacaaaccc
    25321 gcaaggcgtt gcaggcagcg ggcatgactt ttcgcgtcag cgatattccc cgtgatttac
    25381 gcggcggctg cgggttatgc atttggctga cctgtccccc cggcgaggaa atacaatggg
    25441 tgatccctgg gcagactgag tcaatttatt gccagcagga tggtgtctgg cgctgcattg
    25501 cacattacgg ggtttctccg cgttaaggat tcattaaggt cgctacgtat acttctcgat
    25561 atcttattga tttgccttaa agggattacc gtgacaaaaa gtatttcgtt aagtaaacgc
    25621 atctccgtta ttgtgattct ttttgccatt gtggcagtgt gtactttctt cgtgcaatct
    25681 tgcgcccgta aaagcaatca tgcagcatca tttcagaatt accacgccac cattgacggc
    25741 aaagaaattg caggcataac gaataatatc tcttcgctga cctggtcggc acaaagcaat
    25801 actcttttta gcactatcaa caaacctgcc gccattgttg aaatgaccac caatggtgat
    25861 tttatccgca caattcctct ggattttgtt aaggatctcg agacgatcga atacattggc
    25921 gataatcagt ttgtcattag cgatgagcgc gattacgcca tttatgtgat ttcactgacc
    25981 ccaaactcgg aagtaaaaat cctcaaaaaa ataaaaatcc cgctgcaaga ctccccaacc
    26041 aattgcggtt ttgaagggtt ggcttattct cgccaggatc acactttctg gttttttaaa
    26101 gagaaaaatc cgattgaggt ctacaaagtt aacgggctgt taagtagcaa tgaattacac
    26161 atcagcaaag acgaggcact acaacggcaa tttactttag atgatgtgtc cggcgcagaa
    26221 tttaaccagc aaaaaaatac gcttctggtg ctatctcatg aatctcgcgc actccaggaa
    26281 gtaaccctgg ttggcgaggt tatcggtgaa atatcactaa ccaaaggtag tcgaggtttg
    26341 tcgcataaca tcaaacaggc cgaagggatt gcgatggatg cctctggtaa tatttatatc
    26401 gttagcgaac caaatcgttt ttatcgcttt acccctaaat catcgcatta acaggctggc
    26461 atatttcacc tttttgttat taacttgtta acaacaaaaa tgacatacaa ggttaaacat
    26521 gccttcgtcc tcgcatcccg tagaacgctt ttctttcagc accgcgcttt tcgggatgct
    26581 ggttctgacc ttaggtatgg gattaggtcg cttcctttat acgcctatgt tgcccgtcat
    26641 gatggcggaa ggggcgtttt catttagcca gctctcgtgg attgccagcg gcaactatgc
    26701 ggggtatctg gctggcagtc tgctattttc gtttggcgca tttcaccagc cgtcgcgcct
    26761 gcgcccatta ctgttggttt ctgccctggc gagcgggttg ttgatcctcg caatggcatg
    26821 gttgccgcca tttattctgg tgttattgat tcgcttcctg gcgggtgtcg ccagcgccgg
    26881 tatgctgatt tttggttcga cgctgattat gcagcacacg cgccatcctt ttgtgctggc
    26941 agctttgttt tctggcgttg gcattggcat tgcactgggc aacgaatatg ttctggcagg
    27001 cctgcatttt gccttctcct cgcaaacgtt atggcaaggc gccggggcgc tttctggcat
    27061 gatgctgatt gcacttacgc ttttaatgcc ctcgaaaaaa cacgccatcg caccaatgcc
    27121 attggcaaaa acggagcaac agataatgag ctggtggtta ctggctattc tgtacggcct
    27181 tgcgggattt ggttatatca tcgtcgcgac ttacctgccg ctcatggcga aagatgcagg
    27241 ctcaccattg ttaaccgccc atctctggac gttagtcggc ttatcaattg tgccaggctg
    27301 ctttggctgg ctatgggcag caaaacgttg gggggctttg ccttgcctga cggcgaattt
    27361 gctggtgcag gctatcagtg tgctgcttac tctcgccagc gactctcctc tcttgcttat
    27421 cattagtagt atcggttttg gcggtacctt tatgggcacg acatctctgg tgatgactat
    27481 cgcccgccaa ctgagcgttc cgggaaatct caatctttta ggctttgtga cgctcattta
    27541 cggcattgga caaatcctcg gcccggcgct gaccagtatg ctcggcaatg gaacatcggc
    27601 gcttgccggc gccactctct gcggcgcggc ggcgttgttt attgctgcgc ttatcagcac
    27661 agtacaatta tttaagctgc aagtggtcac ttcatgagga gtgtttccct taccactcaa
    27721 gaaactcaac agcaaccgtt gatgtcagcc agaccccgtt ttccgcttgc cagaaaggga
    27781 tgcctcgttt cgccatttcc tgtgctttga cggttaagat aaccggcgat ccgtgtcttg
    27841 ctcccacttt gcgtgccgtt gcttcatcag cagagagatg tacgtaatga cgctcccctg
    27901 caatcagccc ctgttttttt atctcgtcaa ggaaacggct tgccgtaccg tggtagagaa
    27961 actgtggcgg tgttttttcg gcaaaggaaa tggcgacctg cgaagtcgaa tgcccctgaa
    28021 ccgcgcggat gcacctacca tcactggaat aactaaaacg ctttttatcg ctggtggcaa
    28081 ccacagtatc cagcaatgca cgagttaacc gcttgcccgc tttttgggca caaagtatga
    28141 gcttatcgat atccgcccat ccttcacggt caagtacgat accaatcgcc tccggtttgt
    28201 gtcggaggac aaaacttaaa aacttgctcg tatccgccaa ctctttttcg ttgtactttg
    28261 ccatcgcgtt ttcgtccctg gattttctgt ttttatttac tgatgtcatt gtcactgggt
    28321 ttatgaattt tctgcaactc aatcctgcgc agagcctttt ctacacagcg taatttttcc
    28381 gctatgcaaa aaacgcatag caagaaacat acttgcattt tccccatacc cacatggctg
    28441 ataaggtttt tcagctccag gaattttcct ggctcaagca acacacaaac acaaaaaaac
    28501 aatggggatt gttatgacac aacaagggga tgctgtagcc ggggagctgg cgacagaaaa
    28561 agtgggcatt aaagggtatc tggctttttt tctcaccata atcttttttt ccggagtttt
    28621 ttccgggaca gacagctggt ggcgcgtttt tgatttttcc gttctgaacg gatcgtttgg
    28681 gcaactgcct ggtgcgaatg gagccaccac ctcatttcgc ggggcgggtg gcgcgggggc
    28741 taaagatggc tttctctttg cgctggagct ggcaccgtca gtcattctct ctctggggat
    28801 tatttcaatt accgatggtc ttggtggatt acgcgccgcg cagcagttaa tgacgcctgt
    28861 gctaaaaccg ttgttgggca ttccagggat ctgctcgctg gcgctgatcg ctaacctgca
    28921 aaacactgat gccgccgccg gtatgaccaa agagctggcg caggaagggg aaattaccga
    28981 acgcgataaa gtgattttcg ccgcatatca aaccagcggt agcgccatca tcaccaacta
    29041 cttctcttcc ggcgtcgcgg tgttcgcctt tctgggcacg tccgtaatcg tacctttagc
    29101 cgtcatcctg gtgtttaaat ttgtcggagc caacattttg cgcgtctggc ttaacttcga
    29161 agaacgtcgc aacccaacgc aaggagcgca agcatgacaa ctcaggtccg caaaaacgtc
    29221 atggatatgt ttatcgatgg cgcgcgccgt ggctttacta tcgccacgac caacctgctg
    29281 ccaaacgtgg tgatggcgtt tgtcatcatt caggcgctga aaattaccgg cctgctcgac
    29341 tgggtggggc atatctgtga acccgtaatg gcgctgtggg gattacccgg tgaagcggca
    29401 accgtccttt tagccgcatt aatgagtatg ggcggcgcag tcggtgttgc cgccagtctg
    29461 gcgaccgccg gcgcattaac cggtcacgat gtcaccgttt tactgcccgc gatgtattta
    29521 atgggcaacc cggtgcaaaa tgtcggtcgc tgcctgggga cagccgaagt gaatgccaaa
    29581 tattacccgc atatcattac ggtctgcgtg attaacgcat tgctgtcgat ctgggttatg
    29641 cagcttattg tttaacaagg agttaccatg attgattata ccgcagccgg ttttaccctg
    29701 ctgcagggag cgcatttgta tgcgccggaa gatcggggaa tttgcgatgt cctcgtcgct
    29761 aacggcaaaa ttatcgccgt tgccagcaat atcccttctg acattgtacc ggactgcacg
    29821 gttgtcgatc tcagcgggca gatcctctgt cctggtttta ttgatcaaca cgtccatttg
    29881 attggtggcg gcggcgaagc aggtcccacg acgcgcacgc cagaagtggc gctaagtcgc
    29941 ctgacggaag cgggcgttac gtcagtggtt ggtctgctgg gcaccgactc tatctctcgc
    30001 cacccggaat ccctgctcgc caagacccgt gcgctcaatg aagaaggcat cagcgcctgg
    30061 atgctgaccg gcgcttatca tgtcccttcc cgcaccatta caggttccgt ggaaaaagac
    30121 gtggcgatta tcgatcgtgt gattggcgtg aaatgcgcca tctctgatca ccgttctgcc
    30181 gcaccggacg tttatcacct ggctaatatg gcggcggaat cccgcgttgg cggtttgctc
    30241 ggcggtaaac ctggcgtcac cgtgttccac atgggcgaca gcaaaaaggc gttacagcct
    30301 gtctatgacc tgctggaaaa ctgcgatgtg ccgatcagca agttgctgcc gacccacgtt
    30361 aaccgcaacg ttccgttgtt tgagcaggcg ctggagttcg cgcgcaaagg cggcactatc
    30421 gatatcacca gcagcattga cgaaccggtc gcccctgccg aaggtattgc ccgcgccgtt
    30481 caggcgggta ttccgctggc acgcgtcacc ctcagctccg acggcaacgg tagccagccg
    30541 ttcttcgatg acgaagggaa tttaacccat atcggtgttg ccggttttga aacgttgctg
    30601 gaaaccgtgc aggtgctggt caaagactat gatttcagta tcagcgatgc cctgcgcccg
    30661 ctcaccagta gcgtagccgg tttccttaac ctgaccggga aaggcgaaat tctgccaggc
    30721 aatgatgctg acttgctggt catgacgcca gaactgcgca ttgagcaggt atacgctcgc
    30781 ggcaaactga tggtcaaaga cggcaaagcc tgcgtgaatg gaacgtttga aacggcttaa
    30841 acggttgtca gccgcccgaa tggacgtaga atgcccgcaa gggcggctga cagagtaaaa
    30901 cgtaatggat gactgtggtg cgattttgca taatattgaa accaaatggc tttatgattt
    30961 tctgaccctg gaaaaatgcc gcaatttttc ccaggcggca gtcagtcgca acgtctcgca
    31021 accggcattc agccgccgca tccgtgcgct ggaacaggcg attggtgttg aattgtttaa
    31081 ccgccaggta acgccgctgc aactctcaga acaagggaaa atcttccatt cgcagatccg
    31141 ccatctgttg caacagttag agagcaatct ggcagagctg cgtggcggca gcgattacgc
    31201 gcaacgaaaa atcaagatag ccgctgcaca ctctctttcc ctcgggctgt taccgtccat
    31261 tatcagccag atgccgccgc tctttacctg ggcaattgaa gctattgatg tcgatgaagc
    31321 ggtcgataaa ctgcgtgaag ggcaaagtga ctgtattttt tcctttcacg acgaagattt
    31381 gctggaagcg ccgtttgacc acattcgctt atttgaatct caattgttcc ctgtctgcgc
    31441 cagtgacgaa cacggagaag cactttttaa cctcgcgcag ccacactttc cgttactgaa
    31501 ttacagccgc aactcctaca tggggcgatt gattaatcgc accctgacgc gccacagtga
    31561 gttaagtttc agcacctttt ttgtctcttc gatgagcgag cttttaaagc aggttgccct
    31621 cgacggctgt gggattgcct ggctgccgga gtacgccatc caacaagaaa ttcgcagcgg
    31681 gaagctcgtt gtactgaatc gggacgaact ggtgatcccg attcaggcct atgcataccg
    31741 aatgaatacc cgcatgaatc cagttgccga acgcttctgg cgtgaactgc gcgagctgga
    31801 gattgtgctt agctgacatt ctccagcatc gcactacacc aaccgccatt atcccagacc
    31861 agatcaaacg ttagcggttc accgtcgtgg tacagcatta tctcaaggct gatcgccagc
    31921 gtgtggctgt acgcttcttt cagattgacc tgcaacgccg caatccgtgg ctcatgaaat
    31981 aacagtgcag aacgcagctg acggcaatac cagctctgga gatgtttgtc gctctggtta
    32041 tgcacttgcc actcgggcat gcctgaaaca agtaatgacg gtaatgccga atgaggagaa
    32101 ttaaaaagag tgaggagatc gctacatact gatttacgca gcgatgacgt tttattgcct
    32161 gaaatttcag gtaaaacagc ctgaagtgat tggcgcatca ttttgcgcac tcctgacgtt
    32221 tagcaacata tgcggtttgc cctgaacacc gggcaccact gtattgatgc tcattttcca
    32281 tagccattcc cttttgttca ttacatccat gtcagtctga gacaggcctg aatcctgtca
    32341 gcaaagtata aaaccttact tgcctatgaa tatctactga cttttcagga aaaaagtcat
    32401 gatttgataa aaatcatcaa actcaaaaaa aaaccgctag tatattctga atattccgaa
    32461 tagttatgta gatgaaaata cttgtcaccg ccgctactta attccggtgg gaaattattt
    32521 atttccgcat aatcagccat gtattatttg aaacaattag tttgtaaata atatgaattg
    32581 ataacatatc aaatacatca aaaacaccat tcacaggcat tattaacata gacctcaata
    32641 tagcaacatc aaattccaat tagtaataag atatg
//
LOCUS       DAZGAN020000047        32439 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGAN020000047 DAZGAN020000000
VERSION     DAZGAN020000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.047"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(120..1880)
                     /gene="ycaO"
                     /locus_tag="ACHKYO_RS12185"
                     /old_locus_tag="pgaptmp_002437"
     CDS             complement(120..1880)
                     /gene="ycaO"
                     /locus_tag="ACHKYO_RS12185"
                     /old_locus_tag="pgaptmp_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HGU4429274.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            2010..2702
                     /locus_tag="ACHKYO_RS12190"
                     /old_locus_tag="pgaptmp_002438"
     CDS             2010..2702
                     /locus_tag="ACHKYO_RS12190"
                     /old_locus_tag="pgaptmp_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGU4429275.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2901..3989
                     /gene="serC"
                     /locus_tag="ACHKYO_RS12195"
                     /old_locus_tag="pgaptmp_002439"
     CDS             2901..3989
                     /gene="serC"
                     /locus_tag="ACHKYO_RS12195"
                     /old_locus_tag="pgaptmp_002439"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HGU4429276.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIECPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4060..5343
                     /gene="aroA"
                     /locus_tag="ACHKYO_RS12200"
                     /old_locus_tag="pgaptmp_002440"
     CDS             4060..5343
                     /gene="aroA"
                     /locus_tag="ACHKYO_RS12200"
                     /old_locus_tag="pgaptmp_002440"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGU4429277.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5512..6276
                     /gene="ycaL"
                     /locus_tag="ACHKYO_RS12205"
                     /old_locus_tag="pgaptmp_002441"
     CDS             5512..6276
                     /gene="ycaL"
                     /locus_tag="ACHKYO_RS12205"
                     /old_locus_tag="pgaptmp_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HGU4429278.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADYFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6449..7132
                     /gene="cmk"
                     /locus_tag="ACHKYO_RS12210"
                     /old_locus_tag="pgaptmp_002442"
     CDS             6449..7132
                     /gene="cmk"
                     /locus_tag="ACHKYO_RS12210"
                     /old_locus_tag="pgaptmp_002442"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGU4429279.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7243..8916
                     /gene="rpsA"
                     /locus_tag="ACHKYO_RS12215"
                     /old_locus_tag="pgaptmp_002443"
     CDS             7243..8916
                     /gene="rpsA"
                     /locus_tag="ACHKYO_RS12215"
                     /old_locus_tag="pgaptmp_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGU4429280.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9076..9360
                     /gene="ihfB"
                     /locus_tag="ACHKYO_RS12220"
                     /old_locus_tag="pgaptmp_002444"
     CDS             9076..9360
                     /gene="ihfB"
                     /locus_tag="ACHKYO_RS12220"
                     /old_locus_tag="pgaptmp_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HGU4429281.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9567..11831
                     /locus_tag="ACHKYO_RS12225"
                     /old_locus_tag="pgaptmp_002445"
     CDS             9567..11831
                     /locus_tag="ACHKYO_RS12225"
                     /old_locus_tag="pgaptmp_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HGU4429282.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11868..13616
                     /gene="msbA"
                     /locus_tag="ACHKYO_RS12230"
                     /old_locus_tag="pgaptmp_002446"
     CDS             11868..13616
                     /gene="msbA"
                     /locus_tag="ACHKYO_RS12230"
                     /old_locus_tag="pgaptmp_002446"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HGU4429283.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13613..14599
                     /gene="lpxK"
                     /locus_tag="ACHKYO_RS12235"
                     /old_locus_tag="pgaptmp_002447"
     CDS             13613..14599
                     /gene="lpxK"
                     /locus_tag="ACHKYO_RS12235"
                     /old_locus_tag="pgaptmp_002447"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HGU4429284.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            14636..15868
                     /gene="ycaQ"
                     /locus_tag="ACHKYO_RS12240"
                     /old_locus_tag="pgaptmp_002448"
     CDS             14636..15868
                     /gene="ycaQ"
                     /locus_tag="ACHKYO_RS12240"
                     /old_locus_tag="pgaptmp_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HGU4429285.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            15920..16102
                     /gene="ycaR"
                     /locus_tag="ACHKYO_RS12245"
                     /old_locus_tag="pgaptmp_002449"
     CDS             15920..16102
                     /gene="ycaR"
                     /locus_tag="ACHKYO_RS12245"
                     /old_locus_tag="pgaptmp_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HGU4429286.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16099..16845
                     /gene="kdsB"
                     /locus_tag="ACHKYO_RS12250"
                     /old_locus_tag="pgaptmp_002450"
     CDS             16099..16845
                     /gene="kdsB"
                     /locus_tag="ACHKYO_RS12250"
                     /old_locus_tag="pgaptmp_002450"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HGU4429287.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            16999..17881
                     /locus_tag="ACHKYO_RS12255"
                     /old_locus_tag="pgaptmp_002451"
                     /pseudo
     CDS             16999..17881
                     /locus_tag="ACHKYO_RS12255"
                     /old_locus_tag="pgaptmp_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
     gene            complement(17858..18637)
                     /gene="elyC"
                     /locus_tag="ACHKYO_RS12260"
                     /old_locus_tag="pgaptmp_002452"
     CDS             complement(17858..18637)
                     /gene="elyC"
                     /locus_tag="ACHKYO_RS12260"
                     /old_locus_tag="pgaptmp_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HGU4429288.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18773..19558
                     /gene="cmoM"
                     /locus_tag="ACHKYO_RS12265"
                     /old_locus_tag="pgaptmp_002453"
     CDS             18773..19558
                     /gene="cmoM"
                     /locus_tag="ACHKYO_RS12265"
                     /old_locus_tag="pgaptmp_002453"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HGU4429289.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19555..20877
                     /gene="mukF"
                     /locus_tag="ACHKYO_RS12270"
                     /old_locus_tag="pgaptmp_002454"
     CDS             19555..20877
                     /gene="mukF"
                     /locus_tag="ACHKYO_RS12270"
                     /old_locus_tag="pgaptmp_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HGU4429290.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20858..21562
                     /gene="mukE"
                     /locus_tag="ACHKYO_RS12275"
                     /old_locus_tag="pgaptmp_002455"
     CDS             20858..21562
                     /gene="mukE"
                     /locus_tag="ACHKYO_RS12275"
                     /old_locus_tag="pgaptmp_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HGU4429291.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21562..26022
                     /gene="mukB"
                     /locus_tag="ACHKYO_RS12280"
                     /old_locus_tag="pgaptmp_002456"
     CDS             21562..26022
                     /gene="mukB"
                     /locus_tag="ACHKYO_RS12280"
                     /old_locus_tag="pgaptmp_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HGU4429292.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADCAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26283..28130
                     /gene="ldtD"
                     /locus_tag="ACHKYO_RS12285"
                     /old_locus_tag="pgaptmp_002457"
     CDS             26283..28130
                     /gene="ldtD"
                     /locus_tag="ACHKYO_RS12285"
                     /old_locus_tag="pgaptmp_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4429293.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28311..28859
                     /locus_tag="ACHKYO_RS12290"
                     /old_locus_tag="pgaptmp_002458"
     CDS             28311..28859
                     /locus_tag="ACHKYO_RS12290"
                     /old_locus_tag="pgaptmp_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HGU4429294.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28886..29533
                     /gene="gloC"
                     /locus_tag="ACHKYO_RS12295"
                     /old_locus_tag="pgaptmp_002459"
     CDS             28886..29533
                     /gene="gloC"
                     /locus_tag="ACHKYO_RS12295"
                     /old_locus_tag="pgaptmp_002459"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HGU4429295.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29755..30945)
                     /gene="aspC"
                     /locus_tag="ACHKYO_RS12300"
                     /old_locus_tag="pgaptmp_002460"
     CDS             complement(29755..30945)
                     /gene="aspC"
                     /locus_tag="ACHKYO_RS12300"
                     /old_locus_tag="pgaptmp_002460"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HGU4429296.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31130..32218)
                     /gene="ompF"
                     /locus_tag="ACHKYO_RS12305"
                     /old_locus_tag="pgaptmp_002461"
     CDS             complement(31130..32218)
                     /gene="ompF"
                     /locus_tag="ACHKYO_RS12305"
                     /old_locus_tag="pgaptmp_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HGU4429297.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7918 a         7880 c         8711 g         7930 t
ORIGIN      
        1 aaccttataa ttacaattat tttattaatg caaatatatg taaagcgggg cattaaaaaa
       61 acgccccgta atataactca gactaatcat taagcctaca ttgcgtaggc tatttgattt
      121 tattttgccc agaatgctgc tttggcgcgc tgcagctttt cgtaggcctt caacaacgac
      181 tgatgtgcag caaacgcgtg cagatcgcta tctaccggtt gcaggccgta aaacgccgct
      241 tcgccgctca ttgccgcact ggcggcttct acggcatctg cgccgtacat gcgaacaaac
      301 gcattcagat attgcagcgg ctggcgatct tcttcctgtg ccagtaataa caacgtttgc
      361 aggcagcgat aatagttggc gcgttccgga ctaaatactg atgagttaaa ctccatcgtc
      421 cattcggtcc agaccagagc ctgttccaga tcgccaccag ccagcgccag catggctttt
      481 aattcaccga tacgcagggt gtaccaaccg ttatccgacc cggtcgccag acccaacagc
      541 tcacgcacgc gggtaaagtc atcaaaacct tcttcatcca gttgctcgat gaggttcagg
      601 taatcttctt tttcccactc gctgcctggt agcgaaagaa tcgtttcacg taaatggctg
      661 cccatactgt tattcgcgag ccacagatct tcagccggat aaatatcgga catgccaggc
      721 acgataatgc ggcaagcgta gacacccaga tgctcgtaat cggcaatata aacttcttta
      781 tcttcctttt tgaagatagc catcagcgtg gcgaactctt cttccgtggt gccggagaaa
      841 ttccagtcca caaacggata atcggcatcc tgcttgaaca ggtcccagga gattaaaccg
      901 ctggaatcga taaagtgcgt ttcgaggttg gtatgttcag cgacttcttc atcatcgaag
      961 gttggcggag taaacacatc caaatctttc aggccacgac cttgcagcag ctcggtcacg
     1021 gtacgttcca gtgctacgcc aaaatcagga tgcgcaccga aagaggcaaa gcaggtaccg
     1081 ttagcaggat tgaacagtac cacgcaaatc accggatact ggccgccaag cgaaccgtca
     1141 taagcgaaga ttgggaaacc ctccgcttcc agcgtttcga tggcttcaac tactgctggg
     1201 taacgcgcca gcacgtctgc cgggatctct ggcaggctga tgctttcagc aataatgcgg
     1261 tttttcacgt agcgttcgaa aacttcagac aatccctgaa cgcgtgcttc gttgcgggta
     1321 ttacctgcgg acataccgtt ggagacgtac aggttaccaa tgatattcat cggaatataa
     1381 acggtctgat tgtcggactg acgcgtaaac ggcaggccgc aaataccacg atcttcgtta
     1441 ccggattgta ggtcaatcag catgctgccg gtcagttcat tctccggatc ataaaacgcg
     1501 cgcagacggt catcgagtag tccttctggc acatcgtcat tttcggtcag tgggaaccat
     1561 ttttcgttgg gataatgcac gaacggaccg ttggcgatgg tttcgcccag ccagaaatca
     1621 gcaaaaaagt agttggttga gagacgctcg aaatattcac caagtgcaga agccagcgcc
     1681 gcttttttgg ttgcgccttt accgttggta aaacacagtg cgcactcttt gtcgcgaata
     1741 tgtacagacc agacgttagg cacaggattc agccaggagg cctcttcaat ctgaaagccg
     1801 aggtctgaaa gtttttgctg gaagcgagcg atggaatctt ccagagcggc atctttgccg
     1861 gggataaatg tttgcgtcat gaaaatcact ttagtcgtac ggaaagcgcg caataatacg
     1921 ggttttatct caaaggcgct atcaccgccg ccatgccgat gaactgttga ctatgctttt
     1981 agcggataaa ccacgataag agcataaaaa tgaaggcgtt cgatctccac cgtatggcat
     2041 ttgataaagt gccttttgat ttccttggcg aagttgcact acgtagtctt tatacctttg
     2101 tactggtttt tctgttcctc aaaatgaccg gaagacgcgg tgtgcgacag atgtcgctgt
     2161 ttgaagtgtt aatcattctc acgctgggtt cagcggcggg agatgtggcg ttttatgatg
     2221 atgtgccgat ggtgccggta cttatcgtct ttatcactct ggcgttgtta taccgcctgg
     2281 taatgtggtt gatggcgcac agtgaaaaac tggaagattt actggaaggt aagccggttg
     2341 ttattattga agatggcgag ctggcctggt cgaaactcaa taactccaac atgacggaat
     2401 ttgagttctt tatggagcta cgattgcgtg gcgtggagca gctggggcag gtacgtctgg
     2461 cgattctcga aaccaacggg caaatcagtg tctatttctt tgaagatgac aaggtgaaac
     2521 cgggtttact tattttaccc agtgattgta cgcagcgtta caaagtggtg ccggagtcgg
     2581 cggactatgc ctgtattcgt tgtagtgaaa tcattcatat gaaagcgggg gaaaaacaat
     2641 tatgtccgcg ctgtgcaaat ccagaatgga cgaaggcaag tcgggcaaaa cgggtgacct
     2701 gacagtaaaa acatcggctt tttgctaata atccgagaga ttcttttgtg tgatgcaagc
     2761 cacatttttg ccctcaacgg ttttactcat tgcgatgtgt gtcactgaat gataaaaccg
     2821 atagccacag gaataatgta ttacctgtgg tcgcaatcga ttgaccgcgg gttaatagca
     2881 acgcaacgtg gtgaggggaa atggctcaaa tcttcaattt tagttctggt ccggcaatgc
     2941 taccggcaga ggtgcttaaa caggctcaac aggaactgcg cgactggaac ggtcttggta
     3001 cgtcggtgat ggaagtgagt caccgtggca aagagttcat tcaggttgca gaggaagccg
     3061 agaaggattt tcgcgatctt cttaatgtcc cctccaacta caaggtatta ttctgccatg
     3121 gcggtggtcg cggtcagttt gctgcggtac cgctgaatat tctcggtgat aaaaccaccg
     3181 cagattatgt tgatgccggt tactgggcgg caagtgccat taaagaagcg aaaaaatact
     3241 gcacgcctaa tgtctttgac gccaaagtga ctgttgatgg tctgcgcgcg gttaagccaa
     3301 tgcgtgaatg gcaactctct gataatgctg cttatatgca ttactgcccg aatgaaacca
     3361 tcgacggtat cgccatcgac gaaacgccag acttcggcaa agatgtggtg gtcgccgccg
     3421 acttctcttc aaccattctt tcccgtccga ttgacgtcag ccgttatggc gtgatttacg
     3481 ctggcgcgca gaaaaatatc ggcccggctg gcctgacaat cgtcatcgtt cgtgaagatt
     3541 tgctgggcaa agcgaatatc gagtgtccgt cgattctgga ttattccatc ctcaacgata
     3601 acggctccat gtttaacacg ccgccgacat ttgcctggta tctatctggt ctggtcttta
     3661 aatggctgaa agcgaacggc ggtgtagctg aaatggataa aatcaatcag caaaaagcag
     3721 aactgctata tggggtgatt gataacagcg atttctaccg caatgacgtg gcgaaagcta
     3781 accgttcgcg gatgaacgtg ccgttccagt tggcggacag tgcgcttgac aaattgttcc
     3841 ttgaagagtc ttttgctgct ggccttcatg cactgaaagg tcaccgtgtg gtcggcggaa
     3901 tgcgcgcttc tatttataac gccatgccgc tggaaggcgt taaagcgctg acagacttca
     3961 tggttgagtt cgaacgccgt cacggttaat gccgaaattt tgcttaatcc ccacagccag
     4021 cctgtggggt ttttatttct gttgtagaga gttgagttca tggaatccct gacgttacaa
     4081 cccatcgctc gtgtcgatgg cactattaat ctgcccggtt ccaagagcgt ttctaaccgc
     4141 gctttattgc tggcggcatt agcacacggc aaaacagtat taaccaatct gctggatagc
     4201 gatgacgtgc gccatatgct gaatgcatta acagcgttag gggtaagcta tacgctttca
     4261 gccgatcgta cgcgttgcga aattatcggt aacggcggtc cattacacgc agaaggtgcc
     4321 ctggagttat tcctcggtaa cgccggaacg gcaatgcgtc cgctggcggc agctctttgt
     4381 ctgggtagca atgatattgt gctgaccggt gagccgcgta tgaaagaacg cccgattggt
     4441 catctggtgg atgcgctgcg cctgggcggg gcgaagatca cttacctgga acaagaaaat
     4501 tatccgccgt tgcgtttaca gggcggcttt actggcggca acgttgacgt tgatggctcc
     4561 gtttccagcc aattcctcac cgcactgtta atgactgcgc ctcttgcgcc ggaagatacg
     4621 gtgattcgta ttaaaggcga tctggtttct aaaccttata tcgacatcac actcaatctg
     4681 atgaagacgt ttggtgttga aattgaaaat cagcactatc aacaatttgt cgtaaaaggc
     4741 gggcagtctt atcagtctcc gggtacttat ttggtcgaag gcgatgcatc ttcggcttct
     4801 tactttctgg cagcagcagc aatcaaaggc ggcactgtaa aagtgaccgg tattggacgt
     4861 aacagtatgc agggtgatat tcgctttgct gatgtgctgg aaaaaatggg cgcgaccatt
     4921 tgctggggcg atgattatat ttcctgcacg cgtggtgaac tgaacgctat tgatatggat
     4981 atgaaccata ttcccgatgc ggcgatgacc attgccacgg cggcgttatt tgcaaaaggc
     5041 accaccacgc tgcgcaatat ctataactgg cgtgttaaag aaaccgatcg cctgtttgcg
     5101 atggcaacag aactgcgtaa agtcggtgcg gaagtagaag aggggcacga ttacattcgt
     5161 atcactccac cggaaaaact gaactttgcc gagatcgcga catacaatga tcaccggatg
     5221 gcgatgtgtt tctcgctggt ggcgttgtca gatacaccag tgacgattct tgatcccaaa
     5281 tgtacggcca aaacatttcc ggattatttc gagcagttgg cgcggattag ccaggcagcc
     5341 tgaatgaaca acgggcaata aatagccaaa tctttcttta tcaaaacgtc ggcacattgt
     5401 cggcgttttt tttcggacct tgtgagtcat tttgattaat ggtagcgtcg cttgtcaatg
     5461 taagttgttg atacataata tttatatatg attaatcaac ggatgattca catgaagaat
     5521 actaaattac tgctggcgat tgcgacctct gcagcattac tgacagggtg tcaaaatacc
     5581 cacggtattg ataccaatat ggctatcagc tccggtttaa atgcctataa agcagcaaca
     5641 ttaagcgatg ccgatgcaaa agcgattgcc aatcagggct gtgccgaaat ggacagcggc
     5701 aatcaagtcg caagtaaatc cagcaagtac ggtaaacgtc tggcaaaaat cgccaaagca
     5761 ttgggtaaca atattaatgg cacgccggtc aactataagg tttatatgac cagcgacgtc
     5821 aacgcatggg cgatggcgaa cggctgtgtc cgtgtctaca gtggcctgat ggacatgatg
     5881 aatgacaacg aaattgaagg tgttctgggc catgaactgg gccacgtcgc gttgggtcac
     5941 tcgctggctg aaatgaaagc ttcttatgcg atcgttgccg cacgcgatgc catttcagct
     6001 accagcggag tggcttccca gctttcccgc tcacaattgg gtgatatcgc agaaggcgct
     6061 attaatgcta aatactcccg tgataaagag tccgaagcag attatttctc ctttgatctg
     6121 ttgaagaaac gtggcatcag cacccagggg ctggttggca gctttgaaaa actggctagt
     6181 ctggatggcg gtcgcaccca gtccatgttt gactctcacc caccatcaac agagcgtgcg
     6241 caacacatcc gtgatcgtat cgcctctggt aagtaaatca ttgtcatctt tcgggctggt
     6301 cttctgccag cccgctataa ttgcgcaata aatccccatc tgaatacaga caaaactggt
     6361 ttttgcacac aacgttaacg atttgtggcg tcggcgcgta taatgcgcgc ggttatgtta
     6421 acggtacgcc tgttttaagg agataaagat gacggcaatt gccccggtta ttaccattga
     6481 tggcccaagc ggtgcaggga aaggcacctt gtgtaaggct atggcggaag cattgcaatg
     6541 gcatctgctg gattcgggtg caatttatcg cgtactggca ttggcggcat tacatcacca
     6601 tgttgatgtt gcgtcagaag atgcgctggt accgctggca tcccatctgg atgtacgttt
     6661 tgtgtcaacc aatggcaatc tggaagtgat cctcgaaggg gaagatgtca gcggcgaaat
     6721 tcgtactcag gaagtggcga atgcagcttc acaagtcgcg gcattcccac gcgttcgtga
     6781 agcattattg cgtcgccaac gcgcgtttcg cgaattacca ggcctgattg ccgatggccg
     6841 cgacatggga acggtggtat tccctgatgc gccagtgaaa attttccttg acgcctcctc
     6901 ggaagaacgt gcgcatcgcc gcatgctaca gttgcaggag aagggcttta gtgttaactt
     6961 tgagcgcctt ttggccgaga tcaaagaacg cgacgaccgc gatcgtaacc gagcggtagc
     7021 gccactggtt ccggcagccg atgctttagt gttggattcc accaccttaa gcattgagca
     7081 agtgattgaa aaagcgctac aatacgcgcg ccagaaattg gctctcgcat aagcgaccga
     7141 atttgcagta cccccgttgc aatggaatga cagcgggtat gttaaacaac cccatccggc
     7201 atggagccag gtggacgtta aatataaacc tgaagattaa acatgactga atcttttgct
     7261 caactctttg aagagtcctt aaaagaaatc gaaacccgcc cgggttctat cgttcgtggc
     7321 gttgttgttg ctatcgacaa agacgtagta ctggttgacg ctggtctgaa atctgagtcc
     7381 gccatcccgg ctgagcagtt caaaaacgcc cagggcgagc tggaaatcca ggtaggtgac
     7441 gaagttgacg ttgctctgga cgcagtagaa gacggcttcg gtgaaactct gctgtcccgt
     7501 gagaaagcta aacgtcacga agcctggatc acgctggaaa aagcttacga agatgctgaa
     7561 actgttaccg gtgttatcaa cggcaaagtt aagggcggct tcactgttga gctgaacggt
     7621 attcgtgcgt tcctgccagg ttctctggta gacgttcgtc cggtgcgtga cactctgcac
     7681 ctggaaggca aagagcttga atttaaagta atcaagctgg atcagaagcg caacaacgtt
     7741 gttgtttctc gtcgtgccgt tatcgaatcc gaaaacagcg cagagcgcga tcagctgctg
     7801 gaaaacctgc aggaaggcat ggaagttaaa ggtatcgtta agaacctcac tgactacggt
     7861 gcattcgttg atctgggcgg cgttgacggc ctgctgcaca tcactgacat ggcctggaaa
     7921 cgcgttaagc atccgagcga aatcgtcaac gtgggcgacg aaatcactgt taaagtgctg
     7981 aagttcgacc gcgaacgtac ccgtgtatcc ctgggcctga aacagttggg cgaagatccg
     8041 tgggtagcta tcgctaaacg ttatccggaa ggtaccaaac tgactggtcg cgtgaccaac
     8101 ctgaccgact acggctgctt cgttgaaatc gaagaaggcg ttgaaggcct ggtacacgtt
     8161 tccgaaatgg attggaccaa caaaaacatc cacccgtcca aagttgttaa cgttggcgat
     8221 gtagtggaag ttatggttct ggatatcgac gaagaacgtc gtcgtatctc cctgggtctg
     8281 aaacagtgca aagctaaccc gtggcagcag ttcgcggaaa cccacaacaa gggcgaccgt
     8341 gttgaaggta aaatcaagtc tatcactgac ttcggtatct tcatcggcct ggacggcggc
     8401 atcgacggcc tggttcacct gtctgacatc tcctggaacg ttgcaggcga agaagcagtt
     8461 cgtgaataca aaaaaggcga cgaaatcgct gcagttgttc tgcaggttga cgcagaacgt
     8521 gaacgtatct ccctgggcgt taaacagctc gcagaagatc cgttcaacaa ctgggttgct
     8581 ctgaacaaga aaggcgctat cgtaaccggt aaagtaactg cagttgacgc taaaggcgca
     8641 accgtagaac tggctgacgg cgttgaaggt tacctgcgtg cttctgaagc atcccgtgac
     8701 cgcgttgaag acgctaccct ggttctgagc gttggcgacg aagttgaagc taaattcacc
     8761 ggcgttgatc gtaaaaaccg cgcaatcagc ctgtctgttc gtgcgaaaga cgaagctgac
     8821 gagaaagatg caatcgcaac tgttaacaaa caggaagatg caaacttctc caacaacgca
     8881 atggctgaag ctttcaaagc agctaaaggc gagtaattct ctgactcttc gggattttta
     8941 ttccgaagtt tgttgagttt acttgacaga ttgcaggttt cgtcctgtaa tcaagcacta
     9001 agggcggcta cggccgccct taatcaatgc agcaacagca gccgcttaat ttgcctttaa
     9061 ggaaccggag gaatcatgac caagtcagaa ttgatagaaa gacttgccac ccagcaatcg
     9121 cacattcccg ccaagacggt tgaagatgca gtaaaagaga tgctggagca tatggcctcg
     9181 actcttgcgc agggcgagcg tattgaaatc cgcggtttcg gcagtttctc tttgcactac
     9241 cgcgcaccac gtaccggacg taatccgaag actggcgata aagtagaact ggaaggaaaa
     9301 tacgttcctc actttaaacc tggtaaagaa ctgcgcgatc gcgccaatat ttacggttaa
     9361 gtttttactc aaacttgaac gcgagaaaag cacctgtcgg gtgctttttt catttctcta
     9421 atcaggaact ggaagctgcc tcgcagagtt ttgagcagtt ttcacccttt cgttaaattc
     9481 ttctgaatgt gcctcgggga acgcaaaatt cccaccagac aaccgctcaa caaagttgca
     9541 cactttccat aaacagggag gggtgcatga aaataacgac agtcggcgta tgcataattt
     9601 gcggaatttt tccgttgctg attttgcccc aattgcctgg gacattaacc cttgcgtttc
     9661 tgactctctt cgcctgcgta ctggcattta tccctgttaa aaccgtccgt tatatcgcgc
     9721 tgacgttgct gtttttcgtt tggggcatat tatcagcaaa gcaaattttg tgggcaggag
     9781 aaaccttaac tggcgcgacg caggatgcaa ttgttgagat cactgcaact gacggcatga
     9841 ccactcatta cggtcaaatt actcatctac aaggtcgacg tatattccct gcgccaggcc
     9901 ttgtgctgta tggcgaatat cttccgcaag cggtttgtgc tggacaacaa tggtcaatga
     9961 aactcaaagt tcgtgcggtt catggccaac ttaatgatgg cggctttgat agccagcgtt
    10021 atgccattgc ccagcatcaa ccgctcaccg gccgctttct gcaggcaagt gttattgaac
    10081 cgaattgtag cctgcgtgca cagtatctgg cgtcactaca aacaacgctg caaccctatc
    10141 cgtggaatgc ggttattctt ggtttaggta tgggggaacg gttatccgtc cccaaagaaa
    10201 tcaaaaatat catgcgcgat actggaacgg cgcatttaat ggcgatatcg ggattgcaca
    10261 tcgcttttgc ggcgttgctg gctgccggac tcattcgcag tggacaaatt tttctgcctg
    10321 ggcgctggat ccactggcaa ataccattaa ttggtgggat ctgctgtgct gctttttatg
    10381 cctggttgac gggaatgcaa cctcctgcat tgcgtaccgt ggtggcgctt gctacgtggg
    10441 gaatgcttaa gttaagtggg cgacagtgga gtggctggga tgtatggata tgttgtctgg
    10501 cggcaatttt gctgatggat cctgttgcca ttctctcgca aagtttatgg ctctctgccg
    10561 ctgcggtcgc ggcactgatt ttttggtatc agtggtttcc ctgtcctgag tggcaactgc
    10621 cgccggtatt gcgtgcagtt gtttccctca tccatctgca actgggtatc acacttctgc
    10681 ttatgcccgt gcaaatcgtc atatttcatg gcattagtct gacctcgttt attgcaaatc
    10741 tattagcaat tcccttggtg acatttatca cggttccgtt gatcctcgcc gcgatggttg
    10801 tgcatttaag cgggccgtta atcctggagc aagggttatg gtttcttgcc gaccggtctt
    10861 tggctttact tttctggggg ttaaagagtt taccagaagg gtggatcaac attgctgaac
    10921 gttggcaatg gctatcattt tccccatggt tcttactggt ggtatggcga ttaaacgcct
    10981 ggcgaacgtt gccagcaatg tgtgtggctg taggcttgct gatgtgctgg ccgctatggc
    11041 aaaaacctcg acctgacgag tggcaggtgt acatgcttga tgtcgggcaa gggctggcaa
    11101 tggtgatagc cagaaacggc aaagcgattc tctatgacac aggactggcc tggcccgaag
    11161 gggatagtgg gcaacaactg attatcccct ggctccactg gcataatctt gaaccggaag
    11221 gcgttattct gagtcatgaa catctggatc accggggagg gctggactca atattgcaca
    11281 catggccgat gttatggatc agaagtccgt taaactggga acatcatcag ccctgtgtgc
    11341 gtggcgaagc gtggcaatgg caaggattgc gtttcagcgt gcactggcct ttacaagcta
    11401 gcaacgataa aggaaataac cattcctgtg tggttaaggt tgatgacggg acgaatagca
    11461 ttcttctaac cggtgatatt gaagtccccg ctgaacaaaa gatgctaagc cgttactggc
    11521 agcaagtgca gacaacattg cttcaggtac ctcaccatgg cagtaatacc tcatcatcgt
    11581 tgccattaat tcagcgagtg aatggaaaag tggcactcgc atcggcatcg cgctataacg
    11641 catggcgatt gccctctaat aaagttaagc atcgctatca acagcaagga tatcaatggc
    11701 ttgatactcc acatcagggt caagtgacgg tcaatttttc agcgcaaggc tggcggatta
    11761 gcagcctcag agagcaaatt ttacctcgtt ggtatcatca gtggtttggc gtgccagtgg
    11821 ataacgggta gaatatgcgg ctatttcaac aaatgctggt tttttgaatg cataacgaca
    11881 aagatctctc tacgtggcag acgttccgcc gactgtggcc aaccattgcg ccttttaaag
    11941 cgggtctgat cgtggcgggc gtagcgttaa tcctcaacgc agccagcgat accttcatgt
    12001 tatcgctcct taagccactt cttgatgatg gctttggtaa aacagatcgc tccgtgctgg
    12061 tgtggatgcc gctggtggtg atcgggctga tgattttacg tggtatcacc agctatgtct
    12121 ccagctactg tatctcctgg gtatcaggaa aggtggtaat gaccatgcgt cgccgcctgt
    12181 ttggtcacat gatgggaatg ccggtttcat tttttgacaa acagtcaacg gggacgctgt
    12241 tgtcacgtat cacctacgat tccgaacagg ttgcttcttc ttcttccggc gcactgataa
    12301 cggttgtgcg tgaaggtgcg tcaatcatcg gcctgttcat catgatgttc tattacagtt
    12361 ggcaactgtc gatcattttg attgtgctgg caccgattgt ttcgattgcg attcgcgtag
    12421 tatcgaagcg ttttcgcaac atcagtaaaa acatgcagaa caccatgggg caggtgacca
    12481 ccagcgccga acaaatgctg aagggccaca aagaagtatt gattttcggt ggtcaggaag
    12541 tggaaacgaa acgctttgat aaagtcagca accgaatgcg tcttcagggg atgaaaatgg
    12601 tttcagcctc ttccatctct gatccgatca ttcagctgat cgcctctttg gcgctggcgt
    12661 ttgttctgta tgcggcgagc ttcccaagtg tcatggatag cctgactgcc ggtacgatta
    12721 ccgttgtttt ctcttcaatg attgcactga tgcgtccgct gaaatcactg accaacgtta
    12781 acgcccagtt ccagcgcggt atggcggctt gtcagacgct gtttaccatt ctggacagtg
    12841 agcaggagaa agacgaaggt aagcgcgtga tcgagcgtgc gactggcgac gtggaattcc
    12901 gcaatgtcac ctttacttat ccgggacgtg acgtacctgc attgcgtaac atcaacctga
    12961 aaattccggc agggaagacg gttgctctgg ttggacgctc tggttcaggt aaatcaacca
    13021 tcgccagcct gatcactcgt ttttacgata ttgatgaagg cgaaatcctg atggatggtc
    13081 acgatctgcg cgagtatacc ctggcgtcgt tacgtaacca ggttgctctg gtgtcgcaga
    13141 atgtccatct gtttaacgat acggttgcta acaacattgc ttacgcacgg actgaacagt
    13201 acagccgtga gcaaattgaa gaagcggcgc gtatggccta cgccatggac ttcatcaata
    13261 agatggataa cggtctcgat acagtgattg gtgaaaacgg cgtgctgctc tctggcggtc
    13321 agcgtcagcg tattgctatc gctcgagcct tgctgcgtga tagcccgatt ctgattctgg
    13381 atgaagctac ctcggctctg gataccgaat ccgaacgtgc gattcaggcg gcactggatg
    13441 agttgcagaa aaaccgtacc tctctggtga ttgcccaccg cttgtctacc attgaaaagg
    13501 cagacgaaat cgtggtcgtc gaggatggtg tcattgtgga acgcggtacg cataacgatt
    13561 tgcttgagca ccgcggcgtt tacgcgcaac ttcacaaaat gcagtttggc caatgatcga
    13621 aaaaatctgg tctggtgaat cccctttgtg gcggctattg ctgccactct cctggttgta
    13681 tggcctggta agtggcgcga tccgtctttg ctataaacta aaactgaagc gcgcctggcg
    13741 tgcccccgta ccggttgtcg tggttggtaa tctcaccgca ggcggcaacg gaaaaacccc
    13801 ggtcgttgtc tggctggtgg aacagttgca acagcgcggt attcgcgtgg gggtcgtatc
    13861 gcggggatat ggtggtaagg ctgaatctta tccgctgtta ttgtcggcag ataccacaac
    13921 agcacaggcg ggtgatgaac ctgtgttgat ttatcaacgc actgatgcgc ctgttgcggt
    13981 ttctcccgtt cgttctgatg cggtaaaagc cattctggcg caacaccctg atgtgcagat
    14041 catcgtaacc gacgacggtt tacagcatta ccgtctggcg cgtgatgtgg aaattgtcgt
    14101 tattgatggt gtgcgtcgct ttggcaatgg ctggtggttg ccggcggggc caatgcgtga
    14161 gcgagcgggg cgcttaaagt cggttgatgc ggtaatcgtc aacggcggtg tccctcgcag
    14221 cggtgaaatc cccatgcatc tgctgccggg tcaggcggtg aatttacgta ccggtacgcg
    14281 ttgtgacgtt gctcagcttg aacatgtggt ggcgatggca gggattgggc atccgccgcg
    14341 cttttttgcc acgctgaaga tgtgcggcgt acaaccggaa aaatgtgtac cgctggccga
    14401 tcatcagtct ttgaaccatg cggatgtcag cgcgttggta agcaccgggc aaacgctggt
    14461 aatgactgaa aaagatgcgg tgaaatgccg ggcctttgca gaagaaaatt ggtggtattt
    14521 gcctgtagac gcacagcttt caggtgatga accagcgaaa ctgcttacgc aactaacctc
    14581 gctggcttct ggcaactagt tacgccgcgg cagcgttcga ttgatggagt catgaatgtc
    14641 gctgccgcac ctctcccttg ctgatgcgcg taatcttcac cttgccgcac aaggcctgtt
    14701 aaacaaaccc cgccgtcaag cgtcgttgga ggatattccg gcaacgatct cccgcatgtc
    14761 cttgctgcaa atcgatacca tcaatattgt tgcccgtagt ccatatctgg tgcttttcag
    14821 tcgtctggga aattatcctg cccagtggct ggatgagtct ctggcgcgtg gcgaattaat
    14881 ggaatactgg gcgcatgaag cctgctttat gccgcgtagc gactttcgtc ttattcgcca
    14941 ccgcatgctg gcacctgaaa aaatgggctg gaaatacaaa gacgcctgga tgcaggaaca
    15001 tgaggcggaa attgcacagt taattcagca tattcatgat aaggggccgg tacgttcagc
    15061 cgattttgag catcctcgta aaggtgcaag cggctggtgg gaatggaagc cgcataaacg
    15121 gcatctggaa ggtttattta ctgccggaaa ggtgatggtg attgaacggc gcaacttcca
    15181 gcgcgtttat gatttaaccc accgtgtcat gcctgactgg gatgatgagc gcgatctcgt
    15241 ttcgcaaaca gaagcagaaa tcatcatgct ggataacagt gcgcgtagcc tgggaatatt
    15301 ccgcgaacag tggctggcag attactatcg gctgaaacgt ccggcactgg cggcgtggcg
    15361 cgaagcgagg gctgaacagc agcaaatcat tgctgtgcat gttgaaaaat tgggcaatct
    15421 ttggctgcat gctgatttgc tgccgctact cgagcgtgcg ctggccggaa agctcactgc
    15481 aacgcacagc gcggtacttt cgccttttga tcctgttgtc tgggatcgca aacgcgcaga
    15541 gcagcttttt gattttagct accggctgga gtgctatacc ccagcgccga aacgccagta
    15601 tggctatttt gttctgccat tattacatcg tgggcaatta gttgggcgaa tggatgccaa
    15661 aatgcatcgc cagacaggca tccttgaagt tatctctctg tggttacagg aaggcattaa
    15721 accaacgaca atgctgcaaa aagggttacg tcaggcgatt actgatttcg cgagctggca
    15781 gcaggcaacg cgggtgacat taggacgctg cccgcaaggt ctctttactg attgccgcac
    15841 tggctgggaa atagaccccg tcgcataaat gattatgata agatttatgc attcataacc
    15901 cgatccatct ggaggaacta tggatcatcg tttgcttgaa atcattgcct gcccggtttg
    15961 caacggaaaa ctttggtata accaggaaaa acaagagctt atttgcaaac tggataacct
    16021 ggctttcccg ctccgcgatg gcattccagt gttgctggaa acggaagccc gcgtgctgac
    16081 tgctgatgag agtaaatcat gagttttgtg gtcattattc ccgcgcgcta cgcgtccacg
    16141 cgtctgcccg gtaaaccatt ggttgatatt aacggcaaac ccatgattgt tcatgttctt
    16201 gaacgcgcgc gtgaatcagg tgccgagcgc atcatcgtgg caaccgatca tgaggatgtt
    16261 gcccgcgccg ttgaagccgc tggcggtgaa gtatgtatga cgcgcgccga tcatcagtca
    16321 ggaacagaac gtctggcgga agttgtcgaa aaatgcgcat tcagcgacga cacggtgatc
    16381 gttaatgtgc agggtgatga accgatgatc cctgcgacaa tcattcgtca ggttgctgat
    16441 aacctcgctc agcgtcaggt gggtatggcg actctggcgg tgccaatcca caatgcggaa
    16501 gaagcgttta acccgaatgc ggtgaaagtg gttctcgacg ctgaagggta tgcactgtac
    16561 ttctcacgcg ccaccattcc ttgggatcgt gatcgttttg cagaaggcct tgaaaccgtt
    16621 ggcgataact tcctgcgtca tcttggtatt tatggctacc gtgcaggctt tatccgtcgt
    16681 tacgtcaact ggcaggcaag tccgttagaa cacatcgaaa tgttagagca gcttcgtgtt
    16741 ctgtggtacg gcgaaaaaat ccatgttgct gttgctcagg aagttcctgg cacaggtgtg
    16801 gatacccctg aagatcttga gcgcgttcgc gctgaaatgc gctaatttca cttcacgaca
    16861 cttcagccaa ttttgggagg agtgtcgtac cgttacgatt ttcctcaatt tttcttttca
    16921 acaattgatc tcattcaggt gacatctttt atattggcgc tcattatgaa agcagtagct
    16981 tttatgaggg taatctgaat ggaacagctg cgtgccgaat taagccattt actgggcgaa
    17041 aaactcagtc gtattgagtg cgtcaatgaa aaagcggata cggcgttgtg ggctttgtat
    17101 gacagccagg gaaacccaat gccgttaatg gcaagaagct ttagtacgcc cggaaaagcc
    17161 cgacaactgg catggaaaac caccatgctg gcaagaagtg ggactgtccg tatgccgact
    17221 atttatggtg tgatgacgca tgaagaacac cccggccccg atgtcctgct actggagcgg
    17281 atgcgtggtg tttcggtgga ggcaccagcc cgaacaccag aacgctggga acaactcaaa
    17341 gaccaaatcg ttgaagcctt actggcctgg caccgtcagg acagtcgcgg ttgcgtcggc
    17401 gcggtcgaca atactcagga aaatttctgg ccctcatggt accggcaaca tgttgaagtg
    17461 ctatggacca cgctcaatca gttcaataac accggtctga cgatgcagga taagcggatc
    17521 ctgtttcgca ctcgcgaatg tctcccggca ttatttgaag gctttaacga caattgtgtg
    17581 ttgattcacg gtaacttctg tttacgcagc atgctgaaag attcgcgcag cgatcagtta
    17641 ctggcgatgg tcgggccggg actaatgctt tgggcaccgc gagaatacga actgttccga
    17701 ctaatggata attctctggc ggaagatttg ctctggagtt acctgcaacg cgcgccagtg
    17761 gcggagtcgt tcatctggcg gcgtgttatg ggatgaagtt gcgcaactgg ttaataccgg
    17821 acgatttagt cggcgcaact tcgatctggc atcaaaatca ctcttgccgt ggctcgcctg
    17881 acggaccttt cagccactgc cagatgcggc caagcgtttc atagccaaca cgatcgctat
    17941 gcatcagcca gaccggagaa gggattgccc gttcccacgg attaagcggc gagtcgatgg
    18001 ccaactgatt cgccggggct ggtagcggat ttaacccttc ctgctgaaaa aagatcatcg
    18061 cccgcggcag atgtgatgcg gaggtcacca gcaggaaagg ggcgtcacca atcgcctgtt
    18121 tcactgccgc agcttcttct tcggtatctt ttggcaaatc cagggtgata atttgctcgc
    18181 gcggcacgcc cagcgattgc gcaactctgg cacctacttc cgctgtactc accgtattgg
    18241 tttttgctac gcctcccgtg aagatcagtt ttgatcccgg attttcgcgc cataagcgaa
    18301 taccttcatt caggcgtggc agactgttat tgattaaatt agagctcggt gcccactgcg
    18361 ggttccaggt ataaccacct ccgagcacca cgatatagtc cactttttgc gaattattcc
    18421 atgtcggata ggtgctttcg atgggacgca gcaggcggtc ggctaccggt tgcaaactca
    18481 ataacaagag tgccagccac ccaatgctaa taaaaatctt cccggttttt tgaaaacggc
    18541 taaaccacag cagcgccagg ccagcgccaa taattaacaa catcagcgga agtggcagca
    18601 acatattgcc aatcactttt ttcagtgtaa aaagcatcct ttctggttcc ttttttaacc
    18661 atatagcaag ggatcgccag cgatattaca ccagacgggt tcattctcgc cgtggctgtg
    18721 acaaaatagc gtctttatca gaaaacgtct tagccaaccg gtggagaaag caatgcagga
    18781 tcgcaatttt gatgatattg cggaaaagtt ttcccgtaac atttacggca ccaccaaagg
    18841 gcagcttcga caggctattc tgtggcagga tctcgatcac gtgctggcgg aaatgggccc
    18901 gcaaaaactg cgtgtgctgg atgctggcgg tggagaaggg cagaccgcaa tcaaaatggc
    18961 cgagcgtggg catcaggtca ttttatgcga tctttctgcg cagatgatcg accgcgcaaa
    19021 acaggcggca gaagcaaaag gtgtgagcga caacatgcaa tttatacatt gcgccgctca
    19081 ggatgttgct tcgcatttgg aaacgcccgt tgatctgata ttgttccatg cggtgctcga
    19141 gtgggtggcc gatccccgca gtgtattgca gaccctgtgg tcagtgttgc gtccaggcgg
    19201 cgtgttgtcg ttaatgttct acaatgcgca tggtttgttg atgcataaca tggtcgccgg
    19261 gaattttgat tacgtgcagg cgggaatgcc gaaaaagaaa aaacggacgc tttcgccaga
    19321 ttatccacgc gacccggcgc aggtttatct gtggctggaa gaagctggtt ggcaaattat
    19381 gggtaagaca ggcgttcgcg tgtttcatga ttatctgcgc gagaagcacc agcagcgcga
    19441 ctgctatgaa gcattacttg aattagaaac gcgttattgc cgtcaggaac cgtatattac
    19501 cctggggcgt tatattcatg tcaccgcgcg caaaccgcag agcaaggata aagtatgagt
    19561 gaattttccc agacagtccc cgaactggtt gcctgggcca gaaaaaatga cttctccatc
    19621 tcgctgccgg tagaccgact ctcttttctg ctggcggttg ccacgctgaa cggcgagcgt
    19681 ctggatggtg agatgagtga aggcgagctg gtggatgcat tccgccatgt gagtgatgcg
    19741 tttgagcaaa ccagcgaaac catcggcgtg cgcgccaata acgcgatcaa cgacatggtg
    19801 cgtcaacgtc tgctgaaccg ctttaccagc gagcaggcgg aagggaacgc aatttaccgt
    19861 ctgacgccgc tcggcatcgg cattactgac tactacatcc gtcagcgcga gttttctacg
    19921 ctgcgtcttt ctatgcagtt gtcgattgtg gcgggtgagc tcaaacgcgc agcagatgcc
    19981 gccgaagagg gcggtgatga atttcactgg caccgtaatg tctatgcgcc actgaaatat
    20041 tcggtagcag aaattttcga cagtatcgac ctgacgcaac gtctgatgga cgaacagcag
    20101 cagcaggtga aggacgatat cgcccagttg ctgaacaaag actggcgggc ggcgatttcc
    20161 agctgtgaat tgttgctttc ggaaacttcc ggaacgctgc gtgaattgca ggatacgctg
    20221 gaagcggcag gcgacaaatt gcaggctaat ctgttgcgca ttcaggatgc gacgatgacc
    20281 catgacgatc tgcatttcgt cgatcgtctg gtgttcgatc tgcagagcaa actcgatcgt
    20341 attatcagtt ggggccagca atccatcgac ttgtggattg gctacgaccg ccacgtacac
    20401 aaatttattc gtaccgcgat cgatatggat aaaaaccgcg tctttgctca gcggttacgt
    20461 cagtcggtac aaacctattt tgatgagccg tgggcgctaa cttatgccaa tgccgatcgt
    20521 ctgctggata tgcgtgacga agagatggca ctgcgcgatg aagaagtgac tggggaactt
    20581 cctgaggatc tggaatacga agagtttaac gagatccgcg aacagctggc ggcgatcatc
    20641 gaagaacaac ttgccgtgta caaaaccaga caagtgccgc tggatcttgg tctggtggta
    20701 cgcgaatatc tgtcacagta tccgcgtgca cgtcactttg acgttgcgcg cattgttatt
    20761 gatcaggcgg tacgtcttgg cgtagcgcaa gcagatttca ccggactgcc agcgaaatgg
    20821 cagccgatta atgattacgg agccaaggta caggcgcatg tcatcgacaa atattgaaca
    20881 agtgatgccg gttaagctgg cgcaggcgct ggcgaatccg ttatttccgg cgctggacag
    20941 cgccttacgt tcaggccgcc atattggcct cgacgaactg gataatcatg cattcctgat
    21001 ggattttcag gaatatctgg aagagtttta cgcgcgttat aacgttgagc ttattcgcgc
    21061 accagaaggg ttcttctatt tacgcccacg ttccaccacg ctgatccctc gttccgtctt
    21121 gtcggaactg gatatgatgg tcgggaaaat cctctgttat ctctatctca gcccggaacg
    21181 gctggcgaat gaggggattt tcacccagca ggaactgtac gacgaactgc tcaccctggc
    21241 cgatgaagca aaactgctga aactggtgaa caaccgttca accggttcag acgttgaccg
    21301 tcagaagttg caggagaaag tacgttcttc gctcaaccgt ctgcgtcgtt taggcatggt
    21361 gtggtttatg ggccacgaca gcagcaagtt tcgcattacc gaatcggtgt tccgcttcgg
    21421 agccgatgtg cgtgctggcg acgatccccg tgaagcacag cgtcgcctga ttcgtgatgg
    21481 cgaagcaatg ccgattgaaa atcatctgca actcaacgat gaaaccgaag agaatcagcc
    21541 agatagcgga gaggaagaat aatgattgaa cgcggtaaat ttcgctcact gacgctgatt
    21601 aactggaacg gcttttttgc ccgaactttt gaccttgacg agctggtcac gacgctttct
    21661 ggcggtaacg gggcgggtaa atccaccacc atggcggcgt tcgttacggc gctgatcccc
    21721 gacctgaccc tgctgcattt ccgtaacact acggaagccg gggccaccag cggttcgcgc
    21781 gataaaggtc tgcacggtaa gctgaaagcg ggtgtctgtt attcgatgct cgacaccatt
    21841 aactcgcgcc accagcgcgt ggtggtcggt gtgcgtctgc aacaggttgc cggacgcgat
    21901 cgtaaagtgg atatcaagcc gtttgccatt cagggactgc cgatgtcggt gcagccgaca
    21961 cagctggtga ccgaaaccct gaacgaacgc caggcgcgcg tgctgccgct taacgagctg
    22021 aaagacaagc tcgaggcgat ggaaggcgtg cagtttaaac agttcaactc cattactgat
    22081 taccactcgc tgatgttcga tctgggcatc atcgcgcgtc gtctgcgctc cgcatctgac
    22141 cgtagcaaat tctatcgtct gatcgaagct tcgctgtatg gcgggatctc cagtgccatt
    22201 acccgttctc tgcgcgacta cctgttgccg gaaaacagcg gcgtgcgtaa agcgttccag
    22261 gacatggaag cggcgctgcg tgaaaaccgt atgacgctgg aagcgattcg tgtcacccag
    22321 tcggaccgcg acctgtttaa gcatctgatc agcgaagcca ccaactacgt ggcggcggac
    22381 tacatgcgtc acgccaacga gcgccgtgtc catctcgaca aagccctgga gtttcgtcgc
    22441 gagctacata cttcgcgtca gcaactggcg gctgagcagt acaaacacgt cgatatggcg
    22501 cgtgagctgg cagagcacaa cggtgccgaa ggcgatctgg aagcggatta tcaggcggcc
    22561 agcgatcacc tgaacctggt gcaaaccgca ctgcgtcagc aggagaagat cgaacgctac
    22621 gaagcggatc tcgatgagct gcagatccgt ctggaagagc aaaatgaagt ggtggcagaa
    22681 gccatcgaac gccaggaaga gaatgaggct cgtgcggaag ctgccgaact ggaagtggac
    22741 gagctgaaaa gccagcttgc tgactaccag caggcgctgg acgtccagca aacgcgcgcg
    22801 atccagtata accaggcgat tgctgcgctt aatcgtgcca aagaactgtg ccatctgccg
    22861 gacttaaccg ccgactgcgc cgccgaatgg ctggaaacct tccaggcgaa agagctggaa
    22921 gcgactgaaa aaatgctctc tcttgagcag aaaatgagca tggcgcaaac cgcgcacagc
    22981 cagtttgagc aggcttatca gctggtggtg gcaatcaacg gcccgctggc gcgtaacgag
    23041 gcgtgggatg tcgctcgcga actattgcgc gaaggggtcg atcagcgtca cctggcagag
    23101 caggttcaac cgttgcggat gcgcttaagt gaactggaac agcgtctgcg cgagcagcaa
    23161 gaagccgaac gcctgctggc agacttctgc aaacgtcagg gcaagaattt tgatattgat
    23221 gaactggaag ctctgcatca ggagctggaa gcgcgcattg cctctctttc cgatagcgtg
    23281 tctaacgccc gtgaagagcg catggcactg cgccaggaac aggaacagct gcagtctcgc
    23341 attcagagtt tgatgcagcg tgcgccggtt tggctggcag cgcaaaacag tctcaaccag
    23401 ttgagcgagc agtgcggtga agagtttacc tccagccagg acgtcaccga atatctgcaa
    23461 cagttgctgg agcgtgagcg agaggcgatt gttgaacgcg atgaagtggg cgcgcgcaaa
    23521 aacgctgtcg atgaagagat cgaacgttta agccagcctg gcggctctga agatcagcgt
    23581 ctgaacgcgc tggcggagcg ttttggtggt gtgctgctgt cagaaattta tgacgacgtt
    23641 agcctggaag atgcgccgta cttctcagcg ctgtatggcc cgtcacgcca cgccatcgtg
    23701 gtgccagatc tgtcacaggt aactgaacac ctggaaggct tgaccgattg cccggaagat
    23761 ctctatctga tcgaaggaga tccgcagtca ttcgatgaca gcgtgttcag cgttgatgag
    23821 ctggaaaaag cggtagtggt gaaaatcgcc gatcgtcagt ggcgttattc acgtttcccg
    23881 gaagtgccgc tgtttggtcg tgctgcgcgt gaaagccgta ttgaaagcct ccatgccgag
    23941 cgtgaagtgc tttccgaacg cttcgccacg ctctcctttg atgtacagaa aactcagcgt
    24001 ctgcatcagg cgttcagccg ctttatcggc agtcatctgg cggttgcgtt tgagtctgac
    24061 ccggaagcag aaatccgtca actgaacagc cgtcgcgtcg aactggagcg ggcgttaagt
    24121 aatcatgaaa atgataacca gcagcagcgt attcagtttg agcaggcgaa agagggcgtt
    24181 acggcgctga accgcattct gccgcgtctc aacctgttgg ctgatgacag cctggcggat
    24241 cgcgtcgatg aaatccgcga acgtctggat gaagcccagg aagccgcgcg ttttgttcag
    24301 cagtttggca atcaactggc gaaactggaa ccgattgttt cggtattgca gagcgacccg
    24361 gaacagttcg aacagttaaa agaagattac gcgtactctc agcagatgca gcgcgatgcc
    24421 cgtcagcagg cgtttgccct gacggaagtg gtgcagcgtc gtgcgcactt tagctattct
    24481 gactcggcag aaatgcttag cggtaacagc gatctcaacg aaaaactgcg tgaacgtctg
    24541 gaacaggcgg aagcggagcg tacccgcgct cgcgaagcgt tgcgcggtca cgcagcgcag
    24601 ttgagtcagt acaaccaggt gctggcttcg ctgaaaagtt cttacgacac caaaaaagag
    24661 ctactcaacg atctgcaacg tgaattgcag gatatcggcg tgcgtgctga tagcggggca
    24721 gaagagcggg cgcgtattcg ccgtgacgag ctgcatgcgc aactgagcaa taaccgttca
    24781 cgccgcaatc aactggaaaa agcgcttacc ttctgcgaag cggagatgga caacctgacc
    24841 cgcaaactgc gcaagctgga gcgggattac tttgagatgc gcgagcaggt agtgaccgcc
    24901 aaagcgggct ggtgtgcggt gatgcgcatg gtgaaagata acggcgttga gcgccgctta
    24961 caccgtcgtg agctggctta tctctccgct gatgatttgc gttccatgtc ggataaggcg
    25021 ttaggtgcgc tgcgtctggc ggtggcggat aacgaacatc tgcgcgacgt gctgcgcatg
    25081 tcggaagatc cgaaacgtcc ggagcgtaaa attcagttct tcgtggcggt ttatcagcat
    25141 ctgcgtgaac gtattcgtca ggatattatt cgtaccgatg atccggtgga agctatcgaa
    25201 cagatggaga ttgaacttag ccgtctgacc gaagaattaa cctcccgtga acagaaactg
    25261 gcgatcagtt cccgcagcgt ggcgaacatc attcgcaaaa ccattcagcg cgagcagaac
    25321 cgtatccgta tgctcaacca ggggttgcag aacgtatcgt ttggtcaggt gaacagcgtg
    25381 cgtctcaacg tgaacgtgcg tgaaacgcac gccatgctac tggatgtgct ctctgaacag
    25441 cacgagcagc atcaggatct gtttaacagc aaccgtttga ccttctcgga agcgctggcg
    25501 aaactgtatc aacgtcttaa cccgcagatt gatatggggc agcgcacgcc gcagaccatc
    25561 ggtgaagaac tgctggatta ccgcaactat ctggaaatgg aagttgaggt taaccgtggt
    25621 tccgatggct ggctgcgcgc agagtctggt gcattgtcga ccggtgaggc gattggtacc
    25681 ggtatgtcga ttctggtgat ggtggtacaa agctgggaag atgaatctcg ccgcctgcgc
    25741 ggtaaagata tctctccttg ccgcctgctg ttcctcgatg aagcagcgcg actggatgct
    25801 cgttctatcg ccacgctgtt tgaattgtgt gaacgtttgc aaatgcagct catcatcgca
    25861 gcgccggaaa atatcagccc ggagaaaggc accacctata aactggtgcg taaagtcttc
    25921 cagaataccg aacacgttca cgtcgtcggc ctgcgaggat ttgcgccgca actccctgaa
    25981 acgcttccag gaagtgacga agcgccttct caggcgagtt aaaattaagg cggcagcaat
    26041 gccgcctttt ctttttccga aaactccgtt tctgcactaa aaaagtggca cattacggcg
    26101 cgcttttctt taaacttctt tacattaggt tatgtaaaaa cgtatcggcg tttatatact
    26161 gaagataagc ctgatgagta acaggcttgc tcgtcatact ttcgtgagta ttggcgttgt
    26221 acaggcaagt cgtaaaataa cagcctggct attcagagta tgataaaaac agggggcaag
    26281 ggatgttgct taatatgatg tgtggtcgtc ggctgtcggc aatcagtttg tgcctggccg
    26341 taacattcgc tccactgttc aatgcgcagg ccgatgagcc tgaagtaatc cctggcgaca
    26401 gcccggtggc tgtcagtgaa cagggcgagg cactgccgca ggcgcaagcc acggcaataa
    26461 tggcggggat ccagccattg cctgaaggtg cggcagaaaa agcccgcacg caaatcgaat
    26521 ctcaattacc cgcaggttac aagccggttt atcttaacca gcttcaactg ttgtatgccg
    26581 cacgcgatat gcaacccatg tgggaaaacc gtgatgctgt taaagccttc cagcaacagc
    26641 tggcagaggt ggcgattgcc ggtttccagc cgcagtttaa taaatgggta gagttactga
    26701 ccgatcctgg tgttaacggg atggcacgcg acgtagtgct ctctgatgcg atgatgggct
    26761 atctccattt cattgcaaat attccggtca aaggcactcg ctggctatat agcagtaaac
    26821 cttatgcact tgcaacgccg ccgctctcgg tgattaacca atggcagctg gcgctggata
    26881 aaggtcaatt gcctacgttt gttgcaggac tggcaccgca gcatccgcaa tatgcggcga
    26941 tgcatgaatc gttactggcc ttactcagtg acaccaaacc gtggccccaa ctgaccggca
    27001 aagcaacgtt gcgcccaggg cagtggagta acgacgtacc ggcgttgcgc gaaatattgc
    27061 aacgcacagg catgttggac ggggggccga aaattactct acctggcgat gacacgccaa
    27121 ctgacgcggt agtcagccca tccgctgtta ctgttgaaac agcagaaact aagccgatgg
    27181 acaagcaaac gacgtctcgt agtaaacctg cgcctgccgt tcgtgccgcc tacgataatg
    27241 aactggtgga agccgttaaa cgttttcagg catggcaagg attgggggca gatggtgcta
    27301 ttggcccggc aacacgtgac tggttaaacg taacgcccgc ccagcgtgct ggcgtgttgg
    27361 ctctcaacat ccagcgattg cgcttgctgc caacagagct ttctaccggg atcatggtta
    27421 acattccggc ctattcgctg gtctactatc agaacggcaa tcaggtgctg gattcgcgag
    27481 tcattgtcgg tcgccccgat cgcaaaacgc cgatgatgag cagtgccctt aacaacgtag
    27541 tggtaaaccc gccgtggaac gtacctccaa ctctggcacg caaagatatt ctgccaaaag
    27601 tgcgcaacga tccgggatat ctcgaaagcc atggctatac ggtgatgcgc ggctggaaca
    27661 gcagagaagc gattgaccca tggcaggttg actggtctac aatcacggcc tcgaatttac
    27721 cgttccgctt ccagcaggct ccaggcccac ggaactcgct ggggcgctat aaattcaata
    27781 tgccgagttc agaggccatt tatttgcatg acacgccgaa ccacaatctg ttcaagcgtg
    27841 atacacgcgc attgagctca ggctgtgtac gagtgaataa agcttccgat ctggcgaata
    27901 tgctgttgca ggatgcaggc tggaatgaca aacgtatttc tgatgcgctg aagcagggtg
    27961 atacacgtta cgtcaatatt cggcagtcga ttccggtgaa tctctactac ctgacggcct
    28021 ttgttggtgc agatggtcgt acccagtatc gtacagatat ttacaattat gatctgcctg
    28081 cgcgatccag ctcgcaaatc gtatcgaaag cggaacaatt aatcaggtaa atgaagtagt
    28141 tcggggaaat cagttgtcgt aataattagc atgattgggg gcgattctct gcagcccccg
    28201 tcactgctgg ggttgagtca tcttgacgtc tgctttacgg gcggttaagg tgcctcttgt
    28261 gcgccagaag tgcatataaa cgataacatt gacctgtaga cttgattatc atggacaaat
    28321 tcgacgctaa tcgccgcaaa ttgctggcgc ttggtggcgt tgcactcggt gccgccatcc
    28381 tgccgacccc tgcgtttgca acactctcta ccccacgccc gcgcattttg acactcaata
    28441 atcttcatac cggagagtca atcaaagcgg agtttttcga tggcagaggc tatattcagg
    28501 aagaattggc aaaacttaac cattttttcc gcgattaccg cgcgaacaaa ataaagtcca
    28561 tcgacccagg attattcgac cagttgtatc gcctgcaagg gttgttaggc acgcgcaaac
    28621 cggtgcaact catttccggt tatcgttcta ttgataccaa caatgaacta cgcgcccgca
    28681 gccgtggagt agcgaagaaa agctatcaca ctaaaggcca ggcgatggat ttccatattg
    28741 aaggtatcgc gttaagcaat attcgcaaag ccgcgttatc tatgcgcgca ggtggtgtag
    28801 gatattatcc acgtagtaac tttgtgcata ttgataccgg gccagcacgg cactggtagt
    28861 aattgcttaa cgaaacaggg gcagtatgaa ctatcgtatt attccggtca ccgcattctc
    28921 ccagaactgt tcattaatct ggtgtgaaca aacccgtctg gccgcactgg tcgatcctgg
    28981 cggcgatgcg gaaaaaatca aacaggaagt tgatgacagc ggcctgacac tgatgcagat
    29041 cctgctgacg catggtcatc tggaccacgt tggcgcagcg gcggaactgg cgcaacatta
    29101 cggcgtgccg gtttttggcc cggaaaaaga agatgagttc tggctgcaag gcttgcctgc
    29161 gcaaagtcgt atgtttggtc tggaagagtg ccagccgctg acgccagatc gttggctgaa
    29221 cgaaggcgat accatcagca tagggaatgt gactttacag gtgttacatt gccctgggca
    29281 tacgccgggt catgtcgtgt tttttgatga tcgggcaaag ctgctgattt ctggcgatgt
    29341 tattttcaaa ggcggagtag ggcgcagtga cttcccgcgt ggcgatcata atcaactgat
    29401 ttcttcaatc aaagataaat tgctgccact gggggatgac gtgacattta ttccgggtca
    29461 cggaccatta tccacacttg gttatgaacg cctgcataat cccttcctgc aagacgaaat
    29521 gcccgtctgg taaggcacat aaaaaagccc gcttttaatg cgggcctgga tttctggcaa
    29581 agtgcgcttt gtttatgccg gatgcggcac gagcgcctta tccggcctac aaaatcgtgc
    29641 aaattcaaaa tattgcaggg gacgcgtagg cctgataagc gtagcgcatc aggcaatgtt
    29701 gcgtttgtca tcagtctcag cccgcttttc agcgggcttc attgttttta atgcttacag
    29761 cactgccaca atcgcttcgc acagcggagc catgttatct ggtgtcatcc cggccacatt
    29821 tacgcgacca gaagcaaccg catatacgcc aaactcttcg cgcagacgca gcacttgttc
    29881 ttttgtcagg ccactgaagg agaacatgcc gttctgtttg atgataaagc tgaagtcgcg
    29941 gtttgcgcct ttttcctgca gcgtattgac gaacaactga cgcatacgct gaatacgctg
    30001 gcgcatatca gtcagctctt gttcccaaat cgcacgtaac gcatcgttgc tcaggatggt
    30061 ggcaacaaca gaagcgccgt gtgctggtgg gttagagtag ttagcgcgaa tcgccgcttt
    30121 catttggctg aatgcgcgat caacggtttc actgtcggca gcaaccagag tacaagcgcc
    30181 aacacgctcg ttgtacaggc caaagttttt agagtaggaa ctggcaacaa tcagctcttt
    30241 atgcatagcc gcgaaagcgc gcagtccttc agcatcttct tccagaccac gggcaaaacc
    30301 ctggtaagcg aagtcaaaca gcggtaacca gcctttctca actgagagtt gtgccagtgt
    30361 ctgccattgt tccagcgtag ggtcgatacc ggttgggtta tggcagcagc catggaacag
    30421 cactacgtcg ccagcctgag cttcattcag gctgttaatc agtgcatcga agtcaagagt
    30481 gtgattttcc gcatcataat aagcgtattc acgaacttcc agacctgcag agttaaagac
    30541 gctcttatgg ttcggccagc ttgggttgct cacccacact cgcttaacgc tggtattttt
    30601 tgccaggaaa tcggcagcta cgcgtagtgc gccagtgccc cccggagtct gtgccgtgcg
    30661 agcacgtttg tcattgatca gggcgctacc tttaccaaac agcagttcct gagtgcagcg
    30721 accaaattca gggatgccgt caatgccgag gtaatttttg gtggtttcat tttcgagcag
    30781 atactgttca gcctttttca cgctggtcag taccggggtt ttgcccgtct catctttata
    30841 gacaccaatc ccgaggttaa ttttgccggg acgttcatcg gcacgaaaca gatcggccag
    30901 gcccagaatc gggtcggcag gagcggcggt aatgttctca aacatgacga ggttccatta
    30961 tggttacaga agggaagtcc gctatcaggg taacgggaga tttacaaaat tccaactatt
    31021 actgatgaaa acgcaggctg tttttgcaag acgtgagatt gctctggaag gtataaaaaa
    31081 aacaggacca aagtcctgtt ttttcggcat ttaacaaaga ggtatgctat tagaactggt
    31141 aaacgatacc cacagcaacg gtgtcgtctg aacctacgcc cagtttgttg tcagaatcga
    31201 tctggttgat gatgtagtca acataggtgg acatgttttt gttgaagtag taggttgcgc
    31261 ccacttcaaa gtagttcacc agatcaacat caccgatacc ttctacgtct ttcgctttag
    31321 atttggtgta agcgatggac ggacgcagac cgaaatcgaa ctggtattgc gcaactaaca
    31381 gaacgtcttg cgttttgttg gcgaagccgc tggtgtttgt aaatttatta gtgatcggcg
    31441 tagcgttacg ggtttcaccg tagttcgctg ccaggtagat gttgttcgcg tcgtacttca
    31501 gaccagtagc ccactgttca gcttttttac cgttgccaag aggttgagct tcttgcaggt
    31561 tggtacggtc agctgcacca taagcaccaa cgataccaaa gccttcgtat tcgtagctga
    31621 tagaaccgcc aacaccgtcg ccgttagaac ggcgtgcagt gtcacgctcg tttttaccca
    31681 ggtactgaac agcgaagttc aggccatcaa ccagaccaaa gaagttggag ttacgatagg
    31741 tagcaacgcc gccaacacga ccaacgaaga agtcatcgct gtatgcagta tcaccaccaa
    31801 attctggcag catatcggtg taacccagtg catcataaac cacaccgtag ttacggccgt
    31861 aatcgaaaga accaacgtca gcgtatttaa gacccgcgaa tgccagacgc gttttgttac
    31921 cagtttgagc gtcagcgcct tcagagttgt taccctggaa gttatattcc cactgaccat
    31981 aaccggtcag atcggaattg atttgagttt cccctttaaa accaagacgg gcataggtca
    32041 tgtcgccatt gccaccgtaa ctgttttcac cgttaccctt ggaaaaataa tgcagaccaa
    32101 cagctttacc gtacagatct actttgttgc catctttgtt atagatttct gcagcgtttg
    32161 cagtacctgc tactaacaga gcagggacga tcactgccag aatattgcgc ttcatcatta
    32221 tttattaccc tcatggtttt ttttatgaca cctgccactg ccgtcaataa gttctgtcaa
    32281 taaaaattta cggaactatt gatgagagtt tggtgtcttt atgtgtctgc aggcatcttt
    32341 ccattcaaac taacgtttcg ctaccgtgaa agtgctacaa agataaagat ttagtttcaa
    32401 aaagaaaaaa tatgtaacca aaagtaaaaa ttaaggaac
//
LOCUS       DAZGAN020000048        31469 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGAN020000048 DAZGAN020000000
VERSION     DAZGAN020000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.048"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            139..669
                     /locus_tag="ACHKYO_RS12310"
                     /old_locus_tag="pgaptmp_002462"
     CDS             139..669
                     /locus_tag="ACHKYO_RS12310"
                     /old_locus_tag="pgaptmp_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429298.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGESPNDVGICGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLDKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            921..1205
                     /locus_tag="ACHKYO_RS12315"
                     /old_locus_tag="pgaptmp_002463"
                     /pseudo
     CDS             921..1205
                     /locus_tag="ACHKYO_RS12315"
                     /old_locus_tag="pgaptmp_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
     gene            complement(1240..2283)
                     /gene="guaC"
                     /locus_tag="ACHKYO_RS12320"
                     /old_locus_tag="pgaptmp_002464"
     CDS             complement(1240..2283)
                     /gene="guaC"
                     /locus_tag="ACHKYO_RS12320"
                     /old_locus_tag="pgaptmp_002464"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGU4429299.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(2439..2483)
                     /locus_tag="ACHKYO_RS12325"
                     /old_locus_tag="pgaptmp_002465"
     CDS             complement(2439..2483)
                     /locus_tag="ACHKYO_RS12325"
                     /old_locus_tag="pgaptmp_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429300.1"
                     /translation="MIIKTLKMSARKRN"
     gene            2508..3128
                     /gene="coaE"
                     /locus_tag="ACHKYO_RS12330"
                     /old_locus_tag="pgaptmp_002466"
     CDS             2508..3128
                     /gene="coaE"
                     /locus_tag="ACHKYO_RS12330"
                     /old_locus_tag="pgaptmp_002466"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGU4429301.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            3128..3871
                     /gene="zapD"
                     /locus_tag="ACHKYO_RS12335"
                     /old_locus_tag="pgaptmp_002467"
     CDS             3128..3871
                     /gene="zapD"
                     /locus_tag="ACHKYO_RS12335"
                     /old_locus_tag="pgaptmp_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HGU4429302.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            3881..4078
                     /gene="yacG"
                     /locus_tag="ACHKYO_RS12340"
                     /old_locus_tag="pgaptmp_002468"
     CDS             3881..4078
                     /gene="yacG"
                     /locus_tag="ACHKYO_RS12340"
                     /old_locus_tag="pgaptmp_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HGU4429303.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(4178..4567)
                     /gene="mutT"
                     /locus_tag="ACHKYO_RS12345"
                     /old_locus_tag="pgaptmp_002469"
     CDS             complement(4178..4567)
                     /gene="mutT"
                     /locus_tag="ACHKYO_RS12345"
                     /old_locus_tag="pgaptmp_002469"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HGU4429304.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(4627..7332)
                     /gene="secA"
                     /locus_tag="ACHKYO_RS12350"
                     /old_locus_tag="pgaptmp_002470"
     CDS             complement(4627..7332)
                     /gene="secA"
                     /locus_tag="ACHKYO_RS12350"
                     /old_locus_tag="pgaptmp_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGU4429305.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELQDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(7394..7906)
                     /gene="secM"
                     /locus_tag="ACHKYO_RS12355"
                     /old_locus_tag="pgaptmp_002471"
     CDS             complement(7394..7906)
                     /gene="secM"
                     /locus_tag="ACHKYO_RS12355"
                     /old_locus_tag="pgaptmp_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HGU4429306.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(8137..9054)
                     /gene="lpxC"
                     /locus_tag="ACHKYO_RS12360"
                     /old_locus_tag="pgaptmp_002472"
     CDS             complement(8137..9054)
                     /gene="lpxC"
                     /locus_tag="ACHKYO_RS12360"
                     /old_locus_tag="pgaptmp_002472"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HGU4429307.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(9155..10306)
                     /gene="ftsZ"
                     /locus_tag="ACHKYO_RS12365"
                     /old_locus_tag="pgaptmp_002473"
     CDS             complement(9155..10306)
                     /gene="ftsZ"
                     /locus_tag="ACHKYO_RS12365"
                     /old_locus_tag="pgaptmp_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGU4429308.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(10367..11629)
                     /gene="ftsA"
                     /locus_tag="ACHKYO_RS12370"
                     /old_locus_tag="pgaptmp_002474"
     CDS             complement(10367..11629)
                     /gene="ftsA"
                     /locus_tag="ACHKYO_RS12370"
                     /old_locus_tag="pgaptmp_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGU4429309.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(11626..12456)
                     /gene="ftsQ"
                     /locus_tag="ACHKYO_RS12375"
                     /old_locus_tag="pgaptmp_002475"
     CDS             complement(11626..12456)
                     /gene="ftsQ"
                     /locus_tag="ACHKYO_RS12375"
                     /old_locus_tag="pgaptmp_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HGU4429310.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(12458..13378)
                     /gene="ddlB"
                     /locus_tag="ACHKYO_RS12380"
                     /old_locus_tag="pgaptmp_002476"
     CDS             complement(12458..13378)
                     /gene="ddlB"
                     /locus_tag="ACHKYO_RS12380"
                     /old_locus_tag="pgaptmp_002476"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU4429311.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQELVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(13371..14846)
                     /gene="murC"
                     /locus_tag="ACHKYO_RS12385"
                     /old_locus_tag="pgaptmp_002477"
     CDS             complement(13371..14846)
                     /gene="murC"
                     /locus_tag="ACHKYO_RS12385"
                     /old_locus_tag="pgaptmp_002477"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGU4429312.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(14900..15967)
                     /gene="murG"
                     /locus_tag="ACHKYO_RS12390"
                     /old_locus_tag="pgaptmp_002478"
     CDS             complement(14900..15967)
                     /gene="murG"
                     /locus_tag="ACHKYO_RS12390"
                     /old_locus_tag="pgaptmp_002478"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HGU4429313.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPLLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(15964..17208)
                     /gene="ftsW"
                     /locus_tag="ACHKYO_RS12395"
                     /old_locus_tag="pgaptmp_002479"
     CDS             complement(15964..17208)
                     /gene="ftsW"
                     /locus_tag="ACHKYO_RS12395"
                     /old_locus_tag="pgaptmp_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HGU4429314.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(17208..18524)
                     /gene="murD"
                     /locus_tag="ACHKYO_RS12400"
                     /old_locus_tag="pgaptmp_002480"
     CDS             complement(17208..18524)
                     /gene="murD"
                     /locus_tag="ACHKYO_RS12400"
                     /old_locus_tag="pgaptmp_002480"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGU4429315.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(18527..19609)
                     /gene="mraY"
                     /locus_tag="ACHKYO_RS12405"
                     /old_locus_tag="pgaptmp_002481"
     CDS             complement(18527..19609)
                     /gene="mraY"
                     /locus_tag="ACHKYO_RS12405"
                     /old_locus_tag="pgaptmp_002481"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGU4429316.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(19603..20961)
                     /gene="murF"
                     /locus_tag="ACHKYO_RS12410"
                     /old_locus_tag="pgaptmp_002482"
     CDS             complement(19603..20961)
                     /gene="murF"
                     /locus_tag="ACHKYO_RS12410"
                     /old_locus_tag="pgaptmp_002482"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGU4429317.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(20958..22445)
                     /gene="murE"
                     /locus_tag="ACHKYO_RS12415"
                     /old_locus_tag="pgaptmp_002483"
     CDS             complement(20958..22445)
                     /gene="murE"
                     /locus_tag="ACHKYO_RS12415"
                     /old_locus_tag="pgaptmp_002483"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HGU4429318.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(22432..24198)
                     /gene="ftsI"
                     /locus_tag="ACHKYO_RS12420"
                     /old_locus_tag="pgaptmp_002484"
     CDS             complement(22432..24198)
                     /gene="ftsI"
                     /locus_tag="ACHKYO_RS12420"
                     /old_locus_tag="pgaptmp_002484"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HGU4429319.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(24214..24579)
                     /gene="ftsL"
                     /locus_tag="ACHKYO_RS12425"
                     /old_locus_tag="pgaptmp_002485"
     CDS             complement(24214..24579)
                     /gene="ftsL"
                     /locus_tag="ACHKYO_RS12425"
                     /old_locus_tag="pgaptmp_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGU4429320.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(24576..25517)
                     /gene="rsmH"
                     /locus_tag="ACHKYO_RS12430"
                     /old_locus_tag="pgaptmp_002486"
     CDS             complement(24576..25517)
                     /gene="rsmH"
                     /locus_tag="ACHKYO_RS12430"
                     /old_locus_tag="pgaptmp_002486"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGU4429321.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(25519..25977)
                     /gene="mraZ"
                     /locus_tag="ACHKYO_RS12435"
                     /old_locus_tag="pgaptmp_002487"
     CDS             complement(25519..25977)
                     /gene="mraZ"
                     /locus_tag="ACHKYO_RS12435"
                     /old_locus_tag="pgaptmp_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HGU4429322.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(26579..27583)
                     /gene="cra"
                     /locus_tag="ACHKYO_RS12440"
                     /old_locus_tag="pgaptmp_002488"
     CDS             complement(26579..27583)
                     /gene="cra"
                     /locus_tag="ACHKYO_RS12440"
                     /old_locus_tag="pgaptmp_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HGU4429323.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANGPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(27665..27751)
                     /locus_tag="ACHKYO_RS12445"
                     /old_locus_tag="pgaptmp_002489"
     CDS             complement(27665..27751)
                     /locus_tag="ACHKYO_RS12445"
                     /old_locus_tag="pgaptmp_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HGU4429324.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(27763..28254)
                     /gene="ilvN"
                     /locus_tag="ACHKYO_RS12450"
                     /old_locus_tag="pgaptmp_002490"
     CDS             complement(27763..28254)
                     /gene="ilvN"
                     /locus_tag="ACHKYO_RS12450"
                     /old_locus_tag="pgaptmp_002490"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU4429325.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(28257..29981)
                     /gene="ilvI"
                     /locus_tag="ACHKYO_RS12455"
                     /old_locus_tag="pgaptmp_002491"
     CDS             complement(28257..29981)
                     /gene="ilvI"
                     /locus_tag="ACHKYO_RS12455"
                     /old_locus_tag="pgaptmp_002491"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HGU4429326.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            30100..30144
                     /locus_tag="ACHKYO_RS12460"
                     /old_locus_tag="pgaptmp_002492"
     CDS             30100..30144
                     /locus_tag="ACHKYO_RS12460"
                     /old_locus_tag="pgaptmp_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429327.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(30299..31243)
                     /gene="leuO"
                     /locus_tag="ACHKYO_RS12465"
                     /old_locus_tag="pgaptmp_002493"
     CDS             complement(30299..31243)
                     /gene="leuO"
                     /locus_tag="ACHKYO_RS12465"
                     /old_locus_tag="pgaptmp_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HGU4429328.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         7637 a         8760 c         7573 g         7499 t
ORIGIN      
        1 acgggtaaat aatgtatcac caaaaatacc aaataaaggt atttttggta ttttggctaa
       61 tttttgcttt tatccttatt tttcaattgg ataaatagaa agaaaccttt ccttagaaaa
      121 gcaccaagag gtatctttat gtcgcaggga aaagaacctg ttattgaacg tattggaatg
      181 cttacaaagc gctacccttc aagaagtgca gctgccagag cgtggggaat caatatcaac
      241 acattaaaca gctatttcaa gggaggacaa acacaaccag cccccagaga aagtatcctt
      301 atacgaattt ctgaaagtga aggagttagt cttgagtggc ttaaagaggg caagggtgaa
      361 tcaccaaacg atgttggtat ttgtggtaat tttggttgtt ctggtgataa gggagatcaa
      421 cttacacaga tgctttcatt ccttacgaaa gacgaacgag aacagttaac caatctcctt
      481 gctcgcaaag gtgtagagac ggttctgaca ctgctggata aagaaaacca ggatttatta
      541 caactagaag gcaggaagcg cctggctgcg ttgatgattg tagatatgcc agacgattgc
      601 gtcagagaga ttttggaaaa aaccgagaca agtaggcaat caaatccggt agaatctaaa
      661 gcgggttagt ttggcgaggc tggcccgtat gttagttgcc atatagattt aatgaagttt
      721 taaacgcttt aaaaagttga gatcgcagta tcaaaagttt tgccgatttt agaataaacg
      781 attaattatt gcacaatcgg tatcaaatag actttaagcg ctcatccaga aatcacctgt
      841 tttatttcag tatcttacag gtgatttcac ctcctttcac taaaaccctc gttggtatca
      901 aatgattcac ctggttacat atcagtaacg gtcatcccat ctggctggcg ctaaaaaatg
      961 ccggtgagtt tagcccgctc tgtttgcaat tagtgagaac gggagaggca tccggctcgc
     1021 tggatctcat gttagacaac ctcgcccatc atcatcggga taacacaatg gcgctggcgg
     1081 ataacctcgc agccttactg gaaccggcgt tgctgatcat aacgggagga attatcggta
     1141 cgctggtggt ggcgatgtat ctgccaattt tccatttagg cgatgcgatg agtgggatgg
     1201 gataacgctg gcgtgttgct ccacgccagc gttgggagat tacaggttgt tgaagatgcg
     1261 gttttcttgt tcctgcaccc gaataaacgt ggtgcgcttg gtcagttctt tcaggcgtga
     1321 agccccaacg tatgtacaag ctgaacgcaa gccgcccaaa atatcacgcg cggtattttc
     1381 aaccgggcct cgcagcggca gcttaacggt tttaccttct gctgcgcgat attccgcaac
     1441 gccgccaacg tgacgtttca tcgcagactc ggagctcatg ccgtagaaca gcataaattt
     1501 ctcgccgttc tcctcaacga tgcgaccgcc gctctcttcg tggcccgcca gcatgccgcc
     1561 aagcatgacg aaatcggcac cgccgccaaa ggctttcgcg acatcgcccg gcgtggtgca
     1621 gccaccgtcg ctgacgatca ttccgcccag accgtgcgcc gcatcggcac attcgattac
     1681 cgcagaaagc tgcggataac cgacgcctgt tttgacacga gttgtacaaa cagaacctgg
     1741 gccaatgcca actttaacga tatcagcacc ggagaggata agctcctcac acatttcacc
     1801 agtcactacg ttgcccgcac aaatggtttt ggtcggccac gcttcacgcg ctttcgcaac
     1861 gaactgcacg aagtgttcgg aataaccatt cgccacatca atacaaacga agtttaatgc
     1921 cgggttcagg tcgagaattt gtttagtttt ttcgaaatcc gcatcagacg taccggtaga
     1981 aaccatcaca tgtttcagca catcagcgga agaattgttg ataaacgctt gccactcttc
     2041 gacagaatag tgtttatgca cagcagtcaa aatatcaaaa gaagccagcg cagaggccat
     2101 agaaaatgta cctacggtgt ccatatttgc ggcgataatc ggcacgccgg accagctctg
     2161 acctgaatgt ttgaaggtga attgacgttc cagttcaaca tcggaacggc ttttaagagt
     2221 ggagcgttta gggcggatga gaacgtcttt aaaacctaac ttcagatctt cttcaatacg
     2281 catgtgcgga ttcctggggt taatggctat aagggtaaga gcgcaactcc agtgacgcta
     2341 tcatacgcac taatcaatgc ggcgcaagac tgcgaaattg tcttttttta cgctacaatc
     2401 ccataaattt acctggtaaa tagtgagcga acgtttcatc aatttcgctt tcgtgcactc
     2461 atttttaacg ttttgataat catgatgaat tcccgggaat ataaattatg aggtatatag
     2521 ttgccttaac gggaggcatt ggcagtggca agagtaccgt tgccaatgcg tttgctgatc
     2581 tcggaattaa cgtcattgat gccgatatta ttgcgcgtca ggtggttgaa ccaggtgcac
     2641 ctgcgctaca tgccattgct gatcactttg gcgctaacat gattgctgct gatggaacat
     2701 tgcagcgccg ggccttgcgc gagcggatct tcgccaaccc ggaagagaaa aactggctta
     2761 acgccctgct gcatccgctg attcagcaag agacgcaaca ccagatccag caagctactt
     2821 ccccctatgt actgtgggtt gtgccattgc tggtagaaaa ctcactgtat aaaaaagcga
     2881 atcgagtgct ggtggtggat gtcagcccag aaacgcaact taagcgcacc atgcagcgcg
     2941 atgatgtaac tcgcgagcat gtcgaacaaa tccttgctgc tcaggcaacg cgcgaagccc
     3001 gccttgccgt ggcagatgac gtcattgata ataacggcgc accggatgct atcgcatcgg
     3061 atgttgcccg cctgcacgca cactatttgc agcttgcgtc gcagtttgtc tcacaggaaa
     3121 aaccgtaatg cagacccagg tcctttttga acatccacta aatgaaaaaa tgcgtacatg
     3181 gctgcgcatt gagtttttga ttcagcaact caccgttaat ttacccatcg ttgaccacgc
     3241 tggcgcgctg catttcttcc gtaatgtcag tgaattactg gatgttttcg agcgcggcga
     3301 agtccgcact gagctgttga aagaacttga ccggcagcaa cgtaaactcc agacctggat
     3361 tggcgtgcct ggcgtggacc agagccgtat tgaagcatta attcagcagt taaaagcggc
     3421 ggggagcgta ttaatttccg cgccgcgtat cgggcaattt ctgcgtgaag atcgtttgat
     3481 tgctctggtg cgtcagcgac tgagcatccc aggcggctgt tgcagctttg atttacctac
     3541 attgcacatt tggctgcatc taccccaggc gcagcgcgac agccaggtag aaacctggat
     3601 tgccagcctg aacccgctca cccaggcact taccatggtg ctggatttaa ttcgccagtc
     3661 ggcccccttc cgtaaacaaa ccagcctgaa tggtttttat caggataacg gtggcgatgc
     3721 cgacttgctg cgcctgaatc tgtcgctcga ttcacagctt tatccgcaaa tttccggaca
     3781 taagagccgt tttgccattc gttttatgcc gctggacact gaaaacggac aggtaccgga
     3841 acgtctggat ttcgaactgg cctgttgcta aggagtgaaa atgtcagaaa ctattacggt
     3901 gaattgccca acctgcggga aaacggtggt gtggggtgaa atcagcccgt ttcggccatt
     3961 ttgctccaaa cgttgtcagc tgatcgacct cggagaatgg gctgctgaag aaaaacgaat
     4021 ccccagcagt ggcgatcttt ccgaaagcga tgactggagc gaagagccaa agcagtgaca
     4081 tttgtctgat gccgcacgta ggcctgataa gacgcggaca gcgtcgcatc aggcatcttg
     4141 tgcaaatgtc ggatgcggcg tgaacgcctt atccgaccta cagacgctta agcttcgcaa
     4201 ttaccggttc attggctggc ggaaaatcat cggcattaag accgactagc gacatccact
     4261 caccgggttg cccttcttta ccccacggct ccccttccca gctttcgacc agccagaacc
     4321 acagagttat atgcctgtcc gggaattcat attccagttt ttcaaatagc gaaaaatgtt
     4381 gtggggtaat cccgacttct tcctgaagtt cacgcaccac cgcctgttcc ggcgtttcgc
     4441 ccatttcaat tttaccgccg ggaaactcca gtttattcgc catgtgcgca tctgctgcgc
     4501 gacgcgttat aaagatttca ttgttctcgt tgcgaataat acctaccgca atttgcagct
     4561 ttttcattgt cttaaaccta taaaaaaggc gcagaatcct gcgcctttta cttcaactgt
     4621 tagcttttat tgcaggcgac catggcactg cttgtatttt ttaccagaac cgcacgggca
     4681 aggatcgtta cgtcctactt tgcgctcacc ggtttgcgcc gccagtgcag ctgcggctgc
     4741 agagtcgtca tcctgatggc taagctgctg catttgcgct aaacgctcgg cttccatacg
     4801 acgctgttgt tccagctcct caacctcttc aggcatacgt acctggactt tgctcagcgt
     4861 gctgataact tcatatttta acgactccag catcgctgca aacatggaga acgattcacg
     4921 tttgtattcc tgcttcggat ctttctgtgc atagccacgc aggtggatac cctgacgcag
     4981 atagtccatc gccgccaggt gctctttcca cagagagtcg agagtttgca gcatgacgcc
     5041 tttttcgaag tgacgcatca tctcagcacc aaccacttct tctttacgct gatacacttc
     5101 gatggactgc gccagaattc gctcacgcag cgtctcttca tgcagttctg gttctttatc
     5161 cagccactcg gcaattggca aatcgaggtc gaaatcgttc ttcagacgtt cctgcagccc
     5221 cggaatatcc cacatttctt ccagtgactg tggcggaatg taggcatcaa tggtcgcttt
     5281 gaacacatct tcacgaatgc tgttgatggt ttcgctcaca tcgctgacat cctgcagttc
     5341 gttacgctgg gagtaaatgg cgcgacgctg atcgttagcc acgtcatcat attccagcag
     5401 ttgcttacga atgtcgaagt tacggctttc aactttacgc tgggcgttgg caatcgcttt
     5461 agtcacccac gggtgttcaa tggcttcgcc tggcttcata cccagtttac gcatcatgcc
     5521 ggatactcgg tcggaagcaa aaatacgcat cagcgcatct tccatcgaca ggtagaaacg
     5581 ggaagaacca gcatccccct gacgaccaga acgaccgcgc aactggttat cgatacgacg
     5641 ggattcgtga cgctcggtac cgatgatatg caggccacct gcttccagta ccgcatcgtg
     5701 acgtacctgc cagtcggctt taattttttc aatttgctct gcggtcggat tttccagcgc
     5761 ggcaacttct gcctgccagc taccaccgag cacaatatct gtaccacgac ccgccatatt
     5821 ggtcgcgata gtcaccgcag ccggataacc tgcctgagca acaatcgccg cttcgttggc
     5881 gtggaatttg gcgttcagga cgttgtgctt aataccggct ttggtcagtt cgtttgacac
     5941 cagctccgat ttttcgatgg agatagtacc caccagcacc ggctggcctt tcgcagtacg
     6001 ttctttgata tcttcaatga tcgcctgaat tttttccgct tcagtcatgt agaccaggtc
     6061 cggcagatct ttacgaatca ttggacggtt ggtcggaaca acgacggtat ccagcttgta
     6121 gatggagcta aattcgaaag cttcggtatc agcagtaccg gtcatccccg ccagtttttc
     6181 atacagacgg aagtagttct ggaaggtgat cgaagccagc gtttggtttt cgttctggat
     6241 ctgcacacct tctttcgctt ccacagcctg gtgcagacca tcggaccagc gacggccctg
     6301 catggtacga ccggtgtgtt cgtcaacgat gataacttca ccatctttaa cgatgtagtc
     6361 gacgtcgcgg gtaaacagcg catgagcgcg cagcgccgcc gttacgtggt gcatcagcat
     6421 gatgttggcc ggagagtaca gagactcccc ttcatccatg atgccctctt tcaccagcag
     6481 ttcttcaatc agcaccagac cacgttcggt caggttcacc tggcgagatt tttcgtccac
     6541 cgagaagtgg ccttcgccct ggaaggtttc ggagtcttct ttttcctgac ggatcaggtg
     6601 cggaataatt ttattcacgc gtttatacat ttccgagctg tcttctgccg ggccggaaat
     6661 gatcagcggt gtacgcgctt catcgatcag gatggagtcc acttcgtcca ccagcgcata
     6721 gtgcagttta cgctgtacac gttcttcagg gctgaacgcc atattgtcgc gcaggtagtc
     6781 aaagccgtat tcgttgttcg taccgtaagt gatgtcagct gcgtaagctt cgcgctttgc
     6841 cggtgctggc atgcccggca ggttgatacc gacagtcagg ccaaggaatt caaacagcgg
     6901 acggttgttt tcggcgtcac gttgcgccag gtagtcgttg acggtaacta cgtgcacgcc
     6961 tttaccggtt agtgcgttca ggtaagcagg cagcgttgcg gtcagggttt taccttcacc
     7021 ggtacgcatt tcggcgatgc agcgttcgtt aagaaccata ccgccgagta actgaacgtc
     7081 gaagtgacgc ataccaaaga cgcgcttact tgcctcacgt accacggcga aagcttccgg
     7141 gatcagattt tccagcactt cgcctttttc cagacgtgca cgaaactctg cggttttccc
     7201 tttcagttct tcgtcggaga gtttttccat ctccggttcc atggcattga tgatgttgac
     7261 cactttgcgc atccggcgca gggtgcgatc gttacgacta ccgaaaactt tagttaacaa
     7321 tttgattagc ataataaaat ctcaaacgcc ccgcgttgcg gagttaataa aatgaagtaa
     7381 aggtttattg ttgttaggtg aggcgttgag ggccagcacg gatgccttgc gcctggctta
     7441 tccagacggg cgtgctgaat tttgcttgtg gggtaaaatg cgcataatca atgcgataac
     7501 ccttttcaga cggcgtgcct tcctgggtca gcagcgcgct gagcgtatcc agtaatgcaa
     7561 gatgttgcgc ctgaagaggc aaagattctt cagcaacggg cagtgtttgc ggtgccattg
     7621 cgaaagaaag atgacggatt accgtgcgaa tggcatgttg atgccagtaa tcaacggaat
     7681 agttcgaatt cgggcggcgt gtgttcgctt ccagcaaggc caattgacca aagttaactt
     7741 tggctgaagg ctcgtggttg cgggttgtcg cttttgcggg cgcgtttggt tcggcggcgt
     7801 tgctgagcgc aggcaaacct aaactcgccg caaccatccc taataagaga tgcggccaga
     7861 agtagcgttt accaaactgt cgccagcgcg tcagtattcc actcacgtta ttgccatccc
     7921 gatcatattc aaacgtattt agcgcacaaa tcttatcatt aaagcctgca gtccacagca
     7981 cgaatgaaga taagttgtgc ggaaaagtgc ttaagaaaac agcgttcgca ccgtttttta
     8041 tagctcttat aaaattcaaa aaaaaacgac tggttcacct ggccggagag agtgccagat
     8101 ttgccagtcg aattttatac gacagtataa atgtcgttat gccagtacag ctgaaggcgc
     8161 tttgaaggcc aacggcagtt ctgcgtcgtc ctggaaggtc acatattccc aggcttcctg
     8221 tttcgccagg acagcctgca gcagtttgtt attcagtgca tgaccggatt tataagcggt
     8281 aaatgcacca ataatattgt gaccacacat gaacaagtca ccgatcgcat cgagcatttt
     8341 gtgacgcaca aattcgtctt caaaacgcag gccgtcttcg ttcagtacgc gataatcgtc
     8401 aacaacgatg gcacaatcga agctgccgcc caggcacaaa ccacgggact gcagatattc
     8461 gatatcacgc atgaaaccga acgtacgcgc acggctgatc tggcgcataa acgcatcagc
     8521 ggagaagttc atcgcatagc gctggttgct ggaatcaata gccggatggt taaaatcgat
     8581 ggtgaaatcc agcgaaaaac cattgtacgg cttaaattca gcccacttat cgccatcttc
     8641 gacacgaaca gtctctttga tgcgaacaaa ttttttggcg cagttcaact cgtcgatacc
     8701 ggcgtcaagc agcaggtata caaacggagc ggcgctgccg tccatgatcg ggatttccgg
     8761 cgcgttaact tcgataacaa tgttatcgat gcccaagccc gcgagagcag cattgaggtg
     8821 ctctacggtt gaaatccgta catcatgctc gttgaccaga cacgtacaga gcatggtatc
     8881 acgcacagat ttggcatcgg ccgggaaatc taccggtgga ttcaagtcgg tgcgacgata
     8941 gatgaccccg gtgttggccg gcgcagggcg taacgtcagg gtgactttct tgccggtatg
     9001 taaaccgaca cccgtcgcct gaacgatacg tttaagtgtc ctttgtttga tcatcgtatt
     9061 atctcgccaa attacctatc caaccgaagt gtactataca ttcggcgggc cagtttagca
     9121 caaagagcct cgaaacccaa attccagtca attcttaatc agcttgctta cgcaggaatg
     9181 ctgggatatc cagataatcc ggctctttcg cagtttgcgg cgcattgtca ttcacgactt
     9241 tagcaaccgg cttctgctcc tgggtcagcg gagccatccc atgctgctgg tagcgatcca
     9301 tcactggctg ctgaacctgc ttattggtca ccagagtgat ttcaggacgt ttgtccatgc
     9361 cgatacctgt cgcaacaacg gttacgcgca gctcgtcatt catatccggg tcaagagaag
     9421 taccgataac cacagtcgcg ttgtcggaag caaatgcacg gatggtgtta cctaccgttt
     9481 cgaactcatc cagacgcagg tcgaagcccg ccgtgatgtt aaccagcacg ccgcgcgcgc
     9541 cagacaggtc gatatcttcc agcagcggag aagagatagc catttcagca gcttcttccg
     9601 cacggtcttc accgctcgcc acgccagaac ccatcattgc gtagcccatc tcagacatta
     9661 cggtgcgtac gtctgcaaag tccacgttca tcaaacccgg acgagtaatc agttcagcga
     9721 taccttgcac agcgcctttc agtacatcgt tcgctgcgcc aaacgcatcc agcagggaga
     9781 taccgcggcc cagaactttc agcagtttgt cgttcgggat agtgatcaga gagtccacat
     9841 gcttggacag ttcagtgatc ccctgctccg cgaatgccat acgcttcttg ccttcaaagt
     9901 tgaaaggctt agtgacgaca gcaacggtca ggatacccaa atcttttgcc acttcagcga
     9961 cgactggtgc tgcacctgta ccggtaccac cacccatacc cgcagcaata aagaccatgt
    10021 ctgcaccttc cagcgccgca cgcaatgcat cgcgatcctc atcagccgca ttgcggccaa
    10081 cttctggatt agcgccagcg cccagtcctt tggtgatacc gctaccgatt tgaatcgtct
    10141 gtccaaccgc tgttttacgc agcgcttgtg catcggtatt taccgcgaag aattcaacac
    10201 cttcaatgcg ctcgcgcacc atgtgttcaa cagcattacc gccgccgccg ccgacgccga
    10261 tgactttaat caccgcgtca ttggtaagtt ccattggttc aaacatagtt tctctccgat
    10321 ttgtgcctgt cgcctgaggc cgtaatcatc gtcggcctca taaaaattaa aactcttttc
    10381 gcagccaact attgagtcgc ttgatccacg agccaactga tgctgtaaca cgtttttcta
    10441 cttcagcttc accgttaaga tgtgactctt tcccatagtg aagcaatccc accgccgtcg
    10501 aataatacgg ctcctgagca taatccgtta aaccggtaat gttcagcggc gcgccgatac
    10561 gcacttgcgt atgaaacacg cgctgagcac aggctgcaag accttcgatc tgcgctgcgc
    10621 caccggttaa tacaatgcct gccgccaggt gatgtttaac cccttgttgg cgaagctttt
    10681 cctgcaactg caatatctct tcgttgacca ggttgagcag ctcggtatag cgcggctcga
    10741 tcacctctgc cagggtctga cgttgcagac tccgcggcgg acgaccacct acgctcggca
    10801 cttccacgct ctcatctttt ccaacgatgg aacccagcgc acaaccgtgg cgaactttaa
    10861 tcgcttcggc gtcgcttggc ggcgtgccaa aggcgtaagc gatatcactg gtcacgacat
    10921 tgccagcata aggaattacc ttagtgtggc gcaatgcccc accggtataa acggcgatat
    10981 ccattgtacc accaccgata tcgacgacgc agacacccag ttcacgttca tcttccgtca
    11041 ataccgaata acttgatgcc agtccggcaa atatcagttg gtcaactttc agcccacaac
    11101 gttcaaccgc tttgacgatg tttttcgcca tatcgttgtg acatgtgatc aggtgcactt
    11161 ttgcctgcat ccgcacgccc gaaagtccta ccggattctt gatcccttcc tgatagtcaa
    11221 tcgcatactc ttgcgggatc acatgcagca cacgatgctc atcgcgcaca cgcaccgatt
    11281 tcgcggtatg gacgacgttt tccacatctt cttgcgtcac ttcttcttca gaaataggca
    11341 ccataccaat ttcattctgg cagctgatgt gcttaccaga aagcgccaga tataccgaag
    11401 agatctgaca atctgccatc aattctgcct ggtcaatggc gcgttgtacg cacttgacca
    11461 cggattcgag gtcgttcacc ccgcctttat ccataccacg tgacgggcag ctgcccacgc
    11521 caatgatatt gaccataccg tcgggcagaa cttcccctac taaagcggca accttcgcgg
    11581 taccaatctc cagtcctact accagttttc tgtccgtcgc cttgatcatt gttgttctgc
    11641 ctgtgcctga ttttgttgct gagtagattc ctctggcggc aagggcgccc agcctactgc
    11701 cgctccagag tcataacgca aatcaacgta gctaatccgt ttgccatcgg tttgcgcctg
    11761 ctgctgtaaa accggataaa gttctacaaa gcgagccaaa cgtttcatcg tatcgccccg
    11821 gccaagattg agcttaatat cgttattcag cgtcaactgc caggaacgcc gcgcggtcat
    11881 cgccgcttcc ttcagagtaa atctgtcctt tgccagcatc tgccccattt cgcgatagcc
    11941 ctgcaacact tcattggcgc tgccttccgg gccatacagc attggaagca cctgcttgct
    12001 ggtgcgatct ggcggcacgc tgaaggtgtt tccttccgcg tctaccatat gttgatcatt
    12061 ccaccgcgca atcggcacat attcaaccag atgaatcttc aattcatcag gccactgctt
    12121 tctgacgctc acctgcttaa tccacggcag gcgttgttct atttgcgtct ggatgatgtt
    12181 gacatcttgg gtcataaagg tacccggctc acccaatgcc aggatcgact gccggatatc
    12241 gtcattacgc gtgtaatggc gttcaccggt caacaccagc tttgagagcg gcaggcgttg
    12301 cgcatcttcc atccagccca acacgaccca gccgctcacc aacactgtcg ttaaaacggt
    12361 cagcaggaaa aggatccccg ccagacgcgt tccattattg cggcgagaag aaacctcttc
    12421 ttcgctgttt cgcgtgttca gagcagcctg cgacatatta gtccgccagt tccagaattc
    12481 gtactaccaa ctgcgagaag ctcatacctg cctgacgtgc cgccatcggc accaggctgt
    12541 ggctggtcat acccggtgag gtattggctt ccagcagata aaactgtcca tcgctgtcca
    12601 gcataacgtc aatacgtccc catcctttgc aacctaacgt cgtccatgct ttcagcacta
    12661 attcctgcaa attggcctct tgtgacgctt ccagacctgc ggggcagaaa tactgtgtct
    12721 catcagagag atacttcgcc tcataatcat agaaggttcc ggacggttga atacgtattg
    12781 acggtaaaat ttcttcaccg agtatcgcaa ccgtgaactc cggcccactt agccattttt
    12841 caatcaatac ttcttcatcg tgctgaaatg ccaatcttaa tgcatcttgt agagcatttt
    12901 ctgctactac ttttgacatt cccacactgg aaccttcgcg gctcggctta acgataaccg
    12961 gcaaacccag agcagaaatt tctgctaact gcttatcact caggcctttt tcaaactctg
    13021 cgcgggttaa cgcaacccac ggagcgaccg gtaaaccggc accttgccac agcagtttgc
    13081 tgcgtagttt atccattgaa agcgcagatg ccatcactcc actgccggta taaggcaagc
    13141 ccatcagctc gagcatgccc tgcagcgtac catcttcacc gccgcgaccg tgtagcgcga
    13201 taaacacttt ctgaaagccc atcgacttca gttgcgtcac gtcgacttct ttcgggtcga
    13261 caggatacgc atcaataccg ccttcacgca gcccggctaa caccgctgcg ccagaattca
    13321 gagaaacttc ccgctcagcg gaagtcccgc ccaacaggac cgcgatttta tcagtcatgt
    13381 tgttcttcct ccggagtttg cggcttcagt ttgatttcag ctaaagaacg ggcaattttt
    13441 ccgatattac cagccccctg aacgagaatc aggtcgttac cggttaatac cggtgccagc
    13501 atctcggcta cctgcgcagg gtccggcacc agaatgggat caattttccc acgtccacga
    13561 attgtgcgac acagcgaacg gctgtccgct cccggaattg gcgcttcgcc agccggatac
    13621 acttccagca tcaacagggt atcaacctgc gtcagcacat tggcgaaatc atcatacagg
    13681 tcgcgcgtac gggtaaaacg gtgcggctga aacagcatta ccaggttttt atccggccag
    13741 cctgcgcgcg ccgctttaat ggtggcgtca acttccgtcg ggtggtggcc gtagtcatcg
    13801 accagcattg ccgtaccgct tttaccattc actggctcca gcgggaattc accgaggaaa
    13861 tcaaaacggc gaccagtccc ctggaagctc tccagcgcac gcaaaatagc ctcgtcgtca
    13921 attccctctt ccgtagcaac cgcaaccgca gctgcggcgt tcagcgcgtt atgacggcct
    13981 ggcgcattca gggtgacgcg catcggctct ttgtcctggc gcagcagcgt aaagtgcccc
    14041 tgcgggccaa tctgctgata atcttctaca cgcacgtcgg catcttcgct gaagccgtaa
    14101 gttgtggtct gacgtcccac acgcggtaac aattcgcgga tcaccggatc atcaacacac
    14161 atcaccgcac gaccgtaaaa aggcaggttg tgcagaaaat taataaaggt ctgttttaaa
    14221 ttctcaaagt cgccctggta ggtatccatg tggtcggctt cgatattggt gacaatcgcc
    14281 accatcggtt gcagatgcag gaacgatgca tcactctcat ctgcttcggc aatcaggtac
    14341 cgaccatgcc ccaaacgcgc atgaaccccc gccgctttta ccagcccgcc gttaacgaag
    14401 gttgggtcga gccccgcttc tgcgtagatg ctggaaacca tcgcggtagt tgtcgttttg
    14461 ccgtgcgttc ctgcaatggc gatgccatga cgaaaacgca ttaactcagc cagcatttcg
    14521 gcacgacgga tcaccggaat acgcgcttca tgagctgcga caatttccgg gttatcggca
    14581 gaaatcgcgc tggaaacaac aaccacgctg gcatcacgta cgttttccgg gcgatggttg
    14641 aaataaatcg tcgctcccag attcattaac tgctgcgtga ccggatttgg cgctaaatcg
    14701 gaaccactga tctgataacc ttcattggcc agaacttcgg caataccgcc cataccggca
    14761 ccaccgatgc cgacaaaatg tatgtgccga acgcgacgca tttcgggcac gatggaacgc
    14821 agttttgcca attgttgtgt attcattctt tacgccatta acttcttaaa ttcatacgat
    14881 gcaaaagaca tcgctacaat tacgcccggg cagcccggct cacttcattt gccactcgct
    14941 cggtggcatc cggaatggat gcagcgcggg cgcgttctgc catggttaat aaggtttctc
    15001 gcgaccaccc ggccagggtg ttggcgacag catccacgct aagcagtggc tgctcgataa
    15061 ttttggctgc gcccgctttt tccagcggta gcgcattcca gtattgctgg cggtctttat
    15121 gttgaaacgg cacaaacaac gccggtagtc ctgccgcggc gatttcactc accgttaaag
    15181 caccggagcg gcaaacgacg acatccgccc acgcatacgc cgccgccata tcatcaataa
    15241 attcagtcac tttatgctgc ggttgccccg cttcagcata cgcctgttca acggattgtt
    15301 gcgaaccttt gccgctctga tgccagatag tgactgaatc acccagtttc gcagcaacct
    15361 gcggcattgt ctggttaaga atgcgtgcgc cctgggaacc acccactacc agcacacgaa
    15421 ccggaccttc acgtccagcc aaacgttgct gcggcaacgg tagcgccaac acatcggtac
    15481 gcaccgggtt acccaccact tccgcattgg ggaaagcacc tggaaacgcc tgcatcactt
    15541 tggtggcaat cttcgccagc catttattgg ttaagcccgc aataccgttt tgttcatgaa
    15601 gtacaaccgg aatgcctaac gaccacgcgg ccagaccacc tggacctgat acatagccgc
    15661 ccataccgag taccacgtca ggtttgtacg ctttcataat cgcccgcgcc tgacgccagg
    15721 cgttgaagat acgcagcggc gcagctatca gtgcttttat accttttcca cgcagaccag
    15781 agatacgaat gaaatcaatt tcgatgccat gttttggcac taagtccgct tccatacggt
    15841 cggcagtccc cagccagcga acttgccaac cctgagccat tagatggtgc gcaaccgcca
    15901 gtcccgggaa tacatgtcca ccggttccgc ctgccatcac cattaatcgc tttccttgac
    15961 cactcatcgt gaacctcgta caaacgcctg cgctttctcc agacgcgttt cataatcaat
    16021 acgcaacagc atcatgatgg ctgtcgacat aatcagtaag ctcgaaccac cgtaactgat
    16081 cagcggcaat gtcagacctt tggtcggtaa catccccgcc gccgcgccta cgttaaccag
    16141 cgcctggaag ctaaaccaga tgccaataga acaggcgaga aaaccggaaa aacggtggtc
    16201 aatttctaac gctttgcggc caatcgacat cgcgcgaaaa gcgacgaaga ataccattaa
    16261 aagtgccagc accacaccga cataccccag ttcttcgccg ataatggcga aaataaagtc
    16321 agtgtgcgct tccggcagat actccagttt ttgtaccgag ttacctaaac cttgtcccca
    16381 aagttcgccg cgaccaaacg ccatcagcga ttgcgttaac tgatagccgc tgccaaaggg
    16441 atcttcccac gggttccaga atgcggtaac acggcggata cggtacggtt cggcgagtat
    16501 cagcaacaca accgctgaaa tgcccatacc gataatggca atgaactgcc acaatttcgc
    16561 tcccgccagg aacaacatcg ccagcgtagt cacaaacaac accaccaccg taccaaggtc
    16621 tggctgtgcc agcagtaaca ctgccaacac cagaatcacg cccatcggtt tcaggaagcc
    16681 gcgcaggtta ttacgtactt cgtcgccttt acgcaccaga tagttggcga tataacaaaa
    16741 cagcgacagt tttgtcagct ccgcaggctg gatacgcagc aaaccgagat cgatccaacg
    16801 cgatgcccct ttaaccgagc tacccactac cagcacgatc atcagcagga tgatagatcc
    16861 gagcagcatc gtggcactgt agcgttgcca gaactccatc ggcagacgca gcgtaatgat
    16921 cgccagaatg aatgccagga tcagatagac gccatcacgc ttcgcgaaga agaacggatc
    16981 gttggttaag cgttgcccta tgggcattga cgccgaggtc accatgataa agccaatcgc
    17041 cgcaaggccg aaggtcagcc acagtaaggt gcgatcgtac atgatcaggc tgtcggtatc
    17101 tttttcccgc gagcccatca cccagccttt tagcgccgtg gagatccaga ccaggatact
    17161 gaatcctggc aggcgcggca ttttcaggcg agggagagat aaacgcatca acctaactcc
    17221 ttcgccagac gggcaaactc attgcctcgt tgttcaaagt tcttgaactg atcaaggctg
    17281 gcacaggccg gggagagcag aaccatatcg cccggctgaa cacgcggagc cagcaagcgc
    17341 atcgcctgtt ccatagtttc ggtttgttct gccacttccg ggcgtagcgc cgccagctgc
    17401 gcaccgtcac gaccaaaaca gtacaaacgc acgttgtcgc cattcagata gcgcgccagt
    17461 ggactgaagt ccgccgattt accatcgccg cccagcaaca aatgcagcgt gccgtctacc
    17521 tgcaagccat tcaacgccgc ttccgtactg ccgacgttag tggctttcga atcgttaatc
    17581 cagcgtacgc cattgtgctc cagcaccact tcaaagcgat gcggcagacc ggtgaaggtg
    17641 gttaacgctt tcaggctgct ggcgcgcggc aaaccagctg cgtctgccag tgccagtgct
    17701 gccagcgcat tggtgtagtt atgctgcccg gaaagtttca tctctttcac attcagcact
    17761 ttctcgcctt taacccgcag ccaggtttcg ccctgctgat gattcaggtg atagtcaccc
    17821 atgttgacgc caaagctgac gcagcgttca tccgcaccgc gaatcggcat tgttaaggca
    17881 tcatcagcat taaccacgca aactttcgcg ttttcgtaaa tgcgcagttt tgctgcacga
    17941 tactgttgta aaccaaacgg atagcgatcc atatgatctt cagtcacgtt cagaatggtc
    18001 gctgctaccg cctgtaagct ggaggtggtt tccagctgga agctcgacag ttccagcacg
    18061 tacagttcgc actcagcatc aagcagcatc aacgctggca ggccaatatt gccacccaca
    18121 ccaacgttaa cccccgccgc tttcgccatt tcacccacta gcgtggtgac cgtgcttttg
    18181 ccgttagaac cggtaatcgc cacaatcggt gcttgtgctt cgcgacagaa cagctcgata
    18241 tcgccaacga tttcgattcc ggcatcagcg gcagcgctta aggatggatg cgccagtgca
    18301 ataccgggac tggcgacaat cagatctgcc gccatcagcc attcatcatt cagactgccc
    18361 gtgtggcgtt ctacggcttc gggtaattta tccaggccag gcggtgtcat acgcgtatcc
    18421 ataacgcgcg gcgtcacacc gcgagcgagg aaaaagtcca cgcaggaaag cccagtgagg
    18481 cccaggccga taatgacgac atttttaccc tgataatcag ccatgattaa cgtaccttca
    18541 gcgttgccag accaatcaga accagcatca gcgaaataat ccagaaacgc acaatgacgc
    18601 gcggttccgg ccagcctttc agttcatagt ggtgatgaat cggtgccatg cggaaaatac
    18661 gttgtccgcg cagtttaaag gagccgacct gcaggatgac agaaagcgtt tctaccacga
    18721 acacgccccc cataatcacc agcaggaatt cctgacgtag cagtacggcg ataatgccta
    18781 acgcaccacc taacgccagc gaacctacat cgcccataaa gacctgcgcc ggataggtgt
    18841 taaaccacag gaagcccagt cctgccccga ctatcgcggt acagacaata accagttccc
    18901 ctgcgtgtcg cagatacggt atatgcaagt agctggcaaa gttcatattg ccggtcgccc
    18961 acgccaccag cgcaaaacca ccggcgacaa atacggtcgg cataattgcc aggccgtcga
    19021 gaccatcggt caggtttacc gcgttgccag tacccacaat gacgaagtaa gccagcagaa
    19081 tgtagaacag ccccagctgc ggcatcacat ctttaaagaa tgggaccacc agctgcgttg
    19141 cgggcgtgtc tttgccggca aggtacaggg cgaaggcgac acccagcgca atgaccgaca
    19201 tccagaaata cttccaacga gcgatcaacc ctttggtgtc tttacgcacc actttgcgat
    19261 aatcatcaac aaagccaata acaccgtaac ctaccagcac caccaacacg caccagacgt
    19321 acggattgga cgggtaagcc cacagcagta cggagatcac aatcgccgtc aggatcataa
    19381 tcccgcccat ggtcggcgta ccgcgcttgc tgaagtgtga ttcaggaccg tcgttacgca
    19441 ccacctgacc aaaggaaagt ttttgcaaat gagcaatcat acgcgggccc atccacaatg
    19501 agatgaacag cgcggtcagc aagctgacga tggcgcgaaa cgtcagatag gaaaagacgt
    19561 taaagccgga ataatatttg accaaatgtt cggccagcca aactaacatg tcccattctc
    19621 ctgtaaagcg cgtactacct cttccatggc ggcactacgt gaacccttaa ctaaaatcgt
    19681 aattacctgt tgctcagcaa tcagtgattt aagacgcgta attaacgcag ttttatcagc
    19741 aaaatgttcg ccaacgccgc tggcggtgct gatagcatgg ctttgtttac ccacgcttaa
    19801 cacgcggtca ataccagcag cttttgccgc ctcgcccacc tgtacatggc aggcttcgct
    19861 ttcagcgccc agttccgcca tatcgcccac caccagcacg cggtagcccg gcatttcagc
    19921 cagtacctga acagcggcgg tcattgaacc gacattggcg ttgtaggagt cgtcgagcag
    19981 caactggttt tctgccagtt ggatggggaa cagacggcct ggaacagctt tcagatttgc
    20041 cagccccgct ttgatagcat caagcgttgc gcccacggac atggagagcg cagcggctgc
    20101 cagcgcattc gcaatattgt gacgccccgg caacggcagc agaacatcga cgctaccggt
    20161 tggggtttgt agggtaaatt ccgtaccgtg cgaggtcaca tggatattgg tggcggtgaa
    20221 atcgctgttg gcggcattag gtgagaaacg ccacactttg cgtgagccaa ttacgctctg
    20281 ccagttcagc cagtcgttgt tgtcggcgtt cataatggcg ataccgtttt ccggcaggcc
    20341 gctaaagatt tcacctttcg ctttcgcgac acccgcaagc gagccaaaac cttccagatg
    20401 cgccgctgcc aggttgttga ccagcgcagc ttccgggcga gtcagactca cagtccaggc
    20461 tatttcgccc tgatggttcg cgccaagttc aataactgcg taatcgtatt ccggcgttaa
    20521 gcgcaacaac gtcatcggta caccgatgtc gttgttgaga ttgcctgccg tataaagcgt
    20581 gttgccgcac tggcttaaaa tcgccgccgt catctcttta acggaggttt tgccggagga
    20641 ccccgtcaga gcaaccacgc gcgccggaac ttgctggcga acccatgcag ccagttcacc
    20701 aaacgccaga cgcgtatcct tgacgattaa ctgcggcagg tcgatgtcca gcggacggct
    20761 aaccagtagt gcgcctgcgc cgccagcttt cgcctggtcg gcaaaatcgt gggcatcaaa
    20821 acgttcgcct ttcagggcaa caaacaggca gcccggcgtc agttttcggg tatcagtggt
    20881 tacagcatca agggtgatat ctgcaccttg cagttcaccg ttgagaatgt cggtaagttg
    20941 gctaagggtt acgctaatca tgcaatcacc cccagcagac gcgccaccgt gacgcgatcg
    21001 gagtagtcca gacgctgatt gccaacaatc tggtaatctt catggccttt gcccgcgacc
    21061 agtaccacat cattctcttt agcctgcata acggcgcaag tcaccgcttc agcacggcct
    21121 tccatcactt tggcatgtcc ggcatctaac attcccgcca gaatatcgtt gatgatggca
    21181 cgcggttctt cggtacgcgg gttatcgtcc gtcaccaccg ccacgtcagc aaactcttcg
    21241 gcaattgcgc ccatcagtgg acgcttacct ttatcgcgat cgccaccaca gccaaagacg
    21301 caccacagct tgcccgcaca gtgcaggcgc gccgcctgta aggctttttc cagtgcatcc
    21361 ggcgtatgcg cgtaatccac caccaccgtc ggtttgcctg gcgcagtgaa cacttccata
    21421 cgtccgcaaa ccggttgcag acgcgcggcg gttttcagca gatcagccag tggatagccg
    21481 agtgccagca gtgtcgccag cgcgagcagc aggttgctga cgttaaaagc gcccatcaga
    21541 tggctttcaa tttcgccatc gccccaactt gagctaaagc gaatcgtcgc accgctgtcg
    21601 tgatagttca cgtcgatcgc ttttaaccag cgtccgtgac agttcggatt gatatgatct
    21661 tccattgata ccgcaaccgc gtccggcagt tttgccagcc agcggcggcc cacttcatcg
    21721 tcggcgttaa taatcgcctg accgcaatga tgttcagaat aaagcagcca tttcgcagct
    21781 tcgtagtgtt ccatatcacc atgataatca aggtgatcgc ggcttaagtt ggtaaagacc
    21841 gacgccgcaa atttcaatgc cgccacacgg tgctgtacca gcccgtggga ggaaacttcc
    21901 attgcgcaaa acgtcgcgcc ctgctccacc agccccgcca gctcatgctg aacatcgact
    21961 gccgaaccgg tagtattttc tgtcgggatc actttcccaa gcagaccgtt accaacggtg
    22021 cccattaccg cgctggtttc gccaagcagt tggctccact gcgccagcag ctgggtagtc
    22081 gtggttttgc cgttggtgcc cgttacgcct acgagacgta aattgtcaga gggttcatgg
    22141 taaaagcggc ccgccagtgc agataaacgc tcgttgagct ggctgagata gatgaccggt
    22201 acgccgtgca tttcacggat ttcaccgtcg gtcgcctcat ctttcgcctc tgcaataatg
    22261 gcagccacac cttgcgctat cgcctgcggg atatatcgac gcccgtccgc ctgatgacct
    22321 actacagcta caaagagatc gcccgccgca gccacacggc tgtcgagtgt catctctcgc
    22381 agtgctcgcg aaggtgcgtc tggcacccac ggagcaagaa ggtcgcgcaa attacgatct
    22441 gccacctgtc ccctcgcctt gattaatcac aaattcattt ttatcgcccg ttgtcagcgc
    22501 atccggctcg atgttcatgg tacgcaatac gccgcccatg atggcaccaa agaccggcgc
    22561 ggaaacggcg ccgccgtagt atttacccgc ctgcggatcg ttgataacaa caaccagcgc
    22621 gaagcgcggc tgactcgcag gcgcaacgcc tgcggtataa gcaatatatt tattgatgta
    22681 gcgaccgtcc ggcccgacct ttttcgcggt accggtttta atggcgatac gatagccttt
    22741 aatcgccgct ttcacgccgc cgccgcctgg tagcgccacg ctttccatca tatgcaccac
    22801 ggtgcgaaca atggattccg ggaagacacg ttcaccggga accggggggt caactttggt
    22861 aatcgacagt gggcgataaa tgccgtagct gccgatagtt gcgtagactc gcgctaactg
    22921 taacggtgtt accattagcc cgtagccgaa agagaaggtg gccctctcta tgtcagacca
    22981 ccgttgtttt tgaggatata agccactgcg ttctccgacc aaccccaaat tggtcgcttt
    23041 tcccagtcca aaacgtgagt aagtatctac taacgctgag gacggcatcg ctaacgccag
    23101 cttggaaaca ccgacgttac tcgacttctg taataccccg gtcagggtta attcgctgta
    23161 gcgtgccacg tctttgattt cgtggccgtt aattcgataa ggaatggtat tgagtaccga
    23221 gttttcccgc accacgccac gttgcaacgc ggtcattacc accatcggtt taaccgttga
    23281 gcccggttca aacacgtcgg tgatggtacg gttacgcatc gcctctttcg gcgtgccgct
    23341 cagattgtta gggttgtatg acgggctgtt agccatcgcc agcacttcac cggtgttgac
    23401 atccaccagc acggcgctac cggattcagc cttgttaaag gccaccgcgt tgttcagttc
    23461 gcgataaacc agcgcctgca agcgttcatc aatactcaac gccaggttgt gcgccgcctg
    23521 gctgtcagta gaagaaatat cttcaattac gcgaccatag cggtctttac gcacaatgcg
    23581 ctcacccggc tgcccggtaa gccatttatc gaaactcttc tcaacgccct caatcccttg
    23641 actatcgacg ttagtaaagc caatgaggtg agcagtcact tcgccggatg gataataacg
    23701 gcgagattct tcacgcagat gaatccccgg cagtttcagt tttttgatgt agtccgccat
    23761 gtcagggttt acctgacgcg ccagataaat aaagcgccct ttcgggttgg cgttaatgcg
    23821 ggctgaaagc tgatccagcg gaatattgag cgcgttagcc agcgccttcc agcggtcacc
    23881 gacgctgata ccgccagcgt catgcacttc tttcgggtca gcccaaatcg cttttaccgg
    23941 cacgctcacc gctaacgggc gaccagaacg gtcagtaatc atgccgcggg aggtggaaac
    24001 ttgctgaacg cgaagagaac gcatgtcgcc ctctttcacc agcatatccg gggagataac
    24061 ttgtaaccac gctacgcgtc cgagcagaaa agccagcgcc aggagaatac agccgcataa
    24121 caacgcaaaa cgccaactga taaagttggc atgttcttcc tgacgttttg gtttctgcgt
    24181 tttcgccgct gctttcatgc gtcgcgttta tccttatttt tgcactacga tattttcttg
    24241 tgacggatca acatgctgca tttgcagttt ttccgtggcg atcctttcca cccggctatg
    24301 gtcgccgagc gcattctctt caaggatcag gttgcgccat tcaatgtcta aagcatctcg
    24361 ctccagcacc agttgttcgc gctgagcggt cagtaaacgg gtatggtgcg ccgtggttac
    24421 cacggtcacc gccgtcaaaa taatgcaaat gaacaggcag agtggcagct tcccaaatcg
    24481 caaaagatcg tcaccgataa caccaggcaa tgcatggcgc tcgtggcttc ccatcgatcc
    24541 tttaactttg cttagagctt ctgtcactct gctgatcatg cgttcgtcct ctctgcaata
    24601 cgcagaactg aactacgggc acgaggattc tcagccacct cttcttcgcc cggcattaac
    24661 ttgcctaatg ctcgcagctg acggccaccc agttttttga gctgctcttc agtcatcggt
    24721 aaccctgccg gaacttgcgg accgcggctg ttttcacgca taaaacgttt cacaatacgg
    24781 tcttccagcg agtggaagct gatgatcgaa agccgcccac ccggggccag cacgttgagc
    24841 gagcttttta gcgcctgctc tatctcctcc agttcactgt ttacccaaat gcgcaccgcc
    24901 tggaaggtac gggtcgcggg atgtttgaat ttgtctttca ccggcgttgc ggcagccacg
    24961 acttccgcca gttctttggt gcgggtcatc ggctgttcgc ggttacgctc gacaatggcg
    25021 cgggcaatgc gtttggcaaa acgctcttca ccataggttt tcaataccca ggcgatatcg
    25081 gcttcttctg cggtttgcag ccattcagcg gctgactgcc cacgggttgg gtccatacgc
    25141 atgtccagcg gaccatcgcg cataaaggaa aagccgcgct cagcatcatc aagttgcggt
    25201 gaagagacgc caagatcgag gagaatgccg tcgatcttgc cgataagatc gcgctcggca
    25261 acgtattcgc ccagcgcgga gaaaggtccg tggatgatgg agaagcgcgg atcatcaata
    25321 gtcttcgcaa cggcgatagc ctgcgggtcg cgatcgatcg ccagcaaacg cccctcttcg
    25381 ccaagctgcg agaggatcag acgtgagtga ccaccgcgac caaaagtccc atcaatgtag
    25441 atgccatcag gacggatatt gaggccatta acggcttcat ccagcagcac cgtagtatgt
    25501 ttatagtttt ccatcatttt atagagacaa gtcctgcagt cgctccgata agtctccggt
    25561 agccaactgc tctgcgtcga tatcttcctt gacctgttga tgccaggttg tttcatccca
    25621 cagctcaaac ttgttgaact gtccaaccag catcacttct tttgtcagcc cggcatgttg
    25681 ccgcagtact ggcgcgatta acaatcgacc tgcgccatcc atctgacatt cgctggcatg
    25741 acctaacagt aggcgctgca cacggcgctc aaccgggttc atgctcgaca gacgcgataa
    25801 tttttgctcg ataatttccc attcaggcag ggggtaaagc agcaagcacg ggtgatgaat
    25861 gtcaatggtg caaaccattt gaccggcagc gttctcaagc agctgttccc ggtaacgggt
    25921 aggcactgat aagcgccctt tgctgtcgag attgactaac gttgctcccc ggaacatgcc
    25981 agcctcaccc cttattccca ctttgcccca caatttccca ctgaaaggag tttacggagc
    26041 ggaggaaaag cttgtcaagc cagtcacaag gcataccgag gtaaaaaacc cgatgtttac
    26101 ggctaatatc agcgttgttt attttacgtc caacgtgcgc agcaaattaa cgttatgaat
    26161 agctgtaaga aaaaatccct catcgtcatc accgttttaa aatgactcac tattttccac
    26221 agcaaatata aagtgtcagt ttgcgacgcg agcggcattt taggacatat cttccccggt
    26281 taacagtccc tgttgcgtct gtgtggcgct tgatgccagc gggtttgcgc aaaagcaaag
    26341 aaaagtgctt gttaaatcgg caattcatct ctgcatgtaa caaaataatg caaaaacgcg
    26401 ctgtcgatat cacttaatta ttgtgaattt cagctggtga gttaattaac gggcgggttt
    26461 aaaagaactt atcaataata gtcaggaata gtcttattta ctttaaggat attgatgtcc
    26521 attcccgtac tctacgcgcc agagggaaat tcacctggcg cgtattattg ttcgcggctt
    26581 agctacggct gagcacaccg cggcgataga gattacgttt aatgcgcgtt aaaccaggtt
    26641 ttggcttacg cggttcgtcc aggcttgcca ggacaatctc cagcacacgc tctgcgacat
    26701 cgcggtgacg ttgagccacg gccagcaccg ggcactgtaa gaagtcgagc agttcgttat
    26761 cgccaaaggt ggcaattgca aggtcagaag gcagtttacc atcgcgacgt agcgtgacat
    26821 ccatcacccc ttgcaacaac gcaaacgacg ttgtgaacag cgcctgcggc atcggatgcg
    26881 tttccagcca tttttcgaat aactgggcag ccgcctcccg ctcatagctg ttggcataca
    26941 ggaaatgcac ttcgcgcgga tcatctttcc aggcagtacg gaaaccttgt tcacgcagga
    27001 agctgacaga aagctccggc agcgcgccaa gataaagcac cgtctcggcg ggaaacttac
    27061 gtaactcttc cgccagcatt tcggcatcat cctgatcggc accaaccacg ctggtgaaat
    27121 gttcacgatc gagggcgcgg tccagcgcga caatcgggaa cgggccgtta gcccagcgtt
    27181 gataaaaagg atgctcagga ggcaacgatg tcgaaacaat aatggcatcg acctgacgct
    27241 gtaaaaggtg ctcaatacag cgcatttcgt tgtctggctg atcttctgag caggcaatta
    27301 gcagttgata accccgttgc cgcgcctggc gttcaagata gttagcgatg cgggtatagc
    27361 tggtgttctc cagatcgggg atcacaagac caatagaacg tgtgcgtcca gcacgaagcc
    27421 cagctgccac ggcgttcggg tggtaattgt gctcacgcac cacagccatg actttttcaa
    27481 cggttttgtc gctcacacgg tattgcttcg ctttgccgtt aataacatag cttgcagtgg
    27541 tccgcgacac tcccgccagc cgagcgattt catccagttt cacaattgcc ccttgcgtaa
    27601 aatgtaaaaa ccatagccat tgtactggca tgggttaaat tcattaacat ctaagcgcag
    27661 aaaattaact gcggcaactg cttttattcc aggttcccac tgatttcgca aaaaaagccc
    27721 aacgtgacat gttgggctgc aaattgcgca ttcagatcat tctcaacgca ttattttatc
    27781 gccgcgcgaa agtccgacca caccagaacg agcaacttcc acaattttcg ccacctcgcg
    27841 aatcgatgct aaaaatgcat caagcttatc gctggtgcct gctaattgaa cggtataaag
    27901 cgagggtgtg acatcgataa tttgcccacg gaatatttcc gtattacgtt tcacttcgtc
    27961 acgcccgtaa ccgctggcct gaattttcac cagcatgatt tcccgctcaa catgcgcgcc
    28021 ctgccccaac tcgctcacgc gcaagacatc gaccagcttg tgtaattgct tttcgatctg
    28081 ctcaagcact ttttcatcgc ccacggtctg gatggtcata cgcgataatg tcggatcgtc
    28141 ggttggcgca acggtcaggc tttcaatgtt gtagccacgc tgggaaaaaa ggccaatcac
    28201 gcgggataac gcgcctgatt cattttcgag taagactgat aatatccggc gcataatcag
    28261 gttctctccg ttttgcttaa ccacatttca tccattccgc ccccgcgaat ctgcatcggg
    28321 tagacgtgct cactgccatc gacggtaaca tcaacaaaca ccaggcgatt attgcgcacc
    28381 tgttccagcg cctcgctaag tttgctttcc agctcatgcg gatgagagat ctggatcccg
    28441 acgtgaccat aggcttccgc cagacggacg aaatcgggta gtgattgcat ataagattgt
    28501 gaatgacggc cggaatagat catgtcctgc cactgtttca ccatccccag atagcggtta
    28561 ttgagattca ccaccagtac gggcaactcg tattgcaacg cggtagacag ttcctggatg
    28621 ttcatctgaa tactgccgtc gccagtgacg caaaccacgg tttcttctgg caacgccatt
    28681 ttgacgccca gtgccgcagg taaaccaaaa cccatcgtgc cgaggccacc ggaattgatc
    28741 cagcgacgcg gtttgtcgaa tggataataa agtgcagcaa acatctggtg ctgcccgaca
    28801 tcggacgtca cgtaagcgtc cccattagtc aaccgccaaa gagtctcgat caccgcctgc
    28861 ggtttaatct tttcactgtg agtgtcatat ttcaggcact gacgagcgcg ccactgttca
    28921 atttgctgcc accagtcgcg gatctcatcc agtggttgat gggcggattc ttgcgacagg
    28981 agttcaagca tttgttcgag gacctggcga gcatccccca caatcgggat atccgcagtc
    29041 acggttttag aaatggaagt aggatcaata tcgatatgca gaacagtggc atttgggcag
    29101 tactttgcca gattgttcgt cgttcggtca tcaaatcgta ccccgacggc gaaaatcaca
    29161 tccgcgttat gcatcgtcat attggcttcg taggtaccgt gcattcccag catgcccagt
    29221 gcctgacgat gcgttgccgg aaacgccccc agccccatca atgaggaaac aacgggcaga
    29281 ttcaacgcct ccaccgtttc tttcaactgc tgatggcagc ccgccgtgat tgccccaccg
    29341 cctacgtaga caaccggttt ttttgccgct accagcgttt gcagagcacg cttaatttgc
    29401 cctttatgtc cggtagtagt gggattgtaa gaacgcatac tgaccgactc aggccagaca
    29461 tagggtaatt tgttcgccgg attaagaata tctttcggta aatcaacgac cacgggtcct
    29521 gggcgaccac ttgccgccag ccagaaagcc tttttcagca cctgcggaat gtcttccgtt
    29581 tgcttaacca gaaaactgtg tttaaccacc ggtcgcgaaa tccccaccat gtcgcactcc
    29641 tgaaaggcat cgtaacctat caacgaggtc gctacctgcc cggaaaggac aactaatgga
    29701 atggaatcca tataagcggt ggcgatgccg gtaatcgcat tggtcgcccc tggacccgac
    29761 gttaccagca cgacgccgac ttccccggtc gcacgcgcca ggccatcggc catatgcacc
    29821 gccgcctgct catgacgaac taatacatga tcaataccac ccacggtatg caatgcatcg
    29881 taaatatcaa ggactgcgcc tccgggataa ccgaatactt gtttaacgcc ctgatcgata
    29941 agcgatcgga cgaccatctc ggctccagac aacatctcca tggcctgcct cactgtttga
    30001 cggaaaaaat gtgtaaaaca cattaaccgt ccgatcttgc ttaagcaatt ggcagccaga
    30061 gacataaacg cagggtaata agaataaaca ggaggaaagg tgaaaagaaa cgataaaatc
    30121 ctccattacg aaggattaaa ttgataataa atcggctgaa tcccacaact tacagcaatt
    30181 tctctatttt tttaattaat cctaaaatag agagtccaca cattcaggat aaaattctac
    30241 attcaataaa aaatgcagaa taaaccagac attcatgtct ggcctattct gcattcagtt
    30301 agcgtttgca aattgagact aattgttctt ccatccactg atggccttta tcgcgcccgg
    30361 cagcttcatg ccaggagaga taacaggttc tgctgttttg ttttaacggc agcggtaata
    30421 cctgtaattc taaggattca gcgaactctt cagccagcca acgcggcgca atagcgacca
    30481 aatgcgtttg cgacaccacg ctaagtacgc tcatcattgc catgccctga tacgcgatac
    30541 tggcttgctt atctaccgtg tcataccaag gttgactaaa tgacgcgaaa cgatcgagcg
    30601 aaaccgccgc atgttgttcg ttataaacat catgtttcag taacgggcct ttaattgttg
    30661 gatgattttt gctggctacc agcaccattt catctttaaa taatggcacg ctggtaaatt
    30721 caggacgatg gaagtcttca taactaatca caaactccgt ttcctgataa cgcagctgat
    30781 gttcagtgtt ctgatttaat gaagacttga acataacatg tatatttggc gcaatctgct
    30841 caatgtgatt ataaatctgc gaggtcagaa tgctgtctaa cgggctgcaa acacaaagat
    30901 gaaatacacg ttcactgctc gcgggttcaa aacctgaacc aggcaattca ttttgtacta
    30961 gttgcaatgc ctgacgaact gagccaaaaa gttgaaatgc gcgagcagtc ggttgaatac
    31021 cacggccata acgaacaaaa agctcgtcat taaacatcac cttcaggcgt gcaacagcgt
    31081 tactgaccgc aggttgcgac atacccagaa catgagcggc acgggtaatg ttttgctcct
    31141 gcatcacggc atcgaaaacg gttaataagt tgagatcgac catgcgtagc tgtggcttgc
    31201 ttaactccgc cgtctctgaa tgatctgttt gtacctctgg catacttaac tccactgtca
    31261 cacttaactc cctttccctt attggaatgc agagaaataa tcctgaagaa tatgatttat
    31321 catgcatata aaataagaaa aagcaaaatg agtaaaattc ggaaaacata aagatacggg
    31381 ttctgataaa aacagaatcc attgccgtgg aatgagtcat ttaccacagc ataataatcc
    31441 ataatgtttt tgttgcgaaa acaatctaa
//
LOCUS       DAZGAN020000049        30784 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGAN020000049 DAZGAN020000000
VERSION     DAZGAN020000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.049"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            248..631
                     /gene="secE"
                     /locus_tag="ACHKYO_RS12470"
                     /old_locus_tag="pgaptmp_002494"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="ACHKYO_RS12470"
                     /old_locus_tag="pgaptmp_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGU4429329.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="ACHKYO_RS12475"
                     /old_locus_tag="pgaptmp_002495"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="ACHKYO_RS12475"
                     /old_locus_tag="pgaptmp_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGU4429330.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="ACHKYO_RS12480"
                     /old_locus_tag="pgaptmp_002496"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="ACHKYO_RS12480"
                     /old_locus_tag="pgaptmp_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGU4429331.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="ACHKYO_RS12485"
                     /old_locus_tag="pgaptmp_002497"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="ACHKYO_RS12485"
                     /old_locus_tag="pgaptmp_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGU4429332.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHKYO_RS12490"
                     /old_locus_tag="pgaptmp_002498"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHKYO_RS12490"
                     /old_locus_tag="pgaptmp_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGU4429333.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="ACHKYO_RS12495"
                     /old_locus_tag="pgaptmp_002499"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="ACHKYO_RS12495"
                     /old_locus_tag="pgaptmp_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGU4429334.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHKYO_RS12500"
                     /old_locus_tag="pgaptmp_002500"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHKYO_RS12500"
                     /old_locus_tag="pgaptmp_002500"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGU4429335.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHKYO_RS12505"
                     /old_locus_tag="pgaptmp_002501"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHKYO_RS12505"
                     /old_locus_tag="pgaptmp_002501"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGU4429336.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12676..13215
                     /gene="yjaZ"
                     /locus_tag="ACHKYO_RS12510"
                     /old_locus_tag="pgaptmp_002502"
     CDS             12676..13215
                     /gene="yjaZ"
                     /locus_tag="ACHKYO_RS12510"
                     /old_locus_tag="pgaptmp_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HGU4429337.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="ACHKYO_RS12515"
                     /old_locus_tag="pgaptmp_002503"
     CDS             complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="ACHKYO_RS12515"
                     /old_locus_tag="pgaptmp_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HGU4429338.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="ACHKYO_RS12520"
                     /old_locus_tag="pgaptmp_002504"
     CDS             complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="ACHKYO_RS12520"
                     /old_locus_tag="pgaptmp_002504"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HGU4429339.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="ACHKYO_RS12525"
                     /old_locus_tag="pgaptmp_002505"
     CDS             complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="ACHKYO_RS12525"
                     /old_locus_tag="pgaptmp_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HGU4429340.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="ACHKYO_RS12530"
                     /old_locus_tag="pgaptmp_002506"
     CDS             complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="ACHKYO_RS12530"
                     /old_locus_tag="pgaptmp_002506"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HGU4429341.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="ACHKYO_RS12535"
                     /old_locus_tag="pgaptmp_002507"
     CDS             complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="ACHKYO_RS12535"
                     /old_locus_tag="pgaptmp_002507"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGU4429342.1"
                     /translation="MYQPDFPLVPFRSGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="ACHKYO_RS12540"
                     /old_locus_tag="pgaptmp_002508"
     CDS             complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="ACHKYO_RS12540"
                     /old_locus_tag="pgaptmp_002508"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HGU4429343.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19070..19170)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19222..19698)
                     /gene="rsd"
                     /locus_tag="ACHKYO_RS12545"
                     /old_locus_tag="pgaptmp_002509"
     CDS             complement(19222..19698)
                     /gene="rsd"
                     /locus_tag="ACHKYO_RS12545"
                     /old_locus_tag="pgaptmp_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HGU4429344.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19793..20566
                     /gene="nudC"
                     /locus_tag="ACHKYO_RS12550"
                     /old_locus_tag="pgaptmp_002510"
     CDS             19793..20566
                     /gene="nudC"
                     /locus_tag="ACHKYO_RS12550"
                     /old_locus_tag="pgaptmp_002510"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HGU4429345.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20606..21670
                     /gene="hemE"
                     /locus_tag="ACHKYO_RS12555"
                     /old_locus_tag="pgaptmp_002511"
     CDS             20606..21670
                     /gene="hemE"
                     /locus_tag="ACHKYO_RS12555"
                     /old_locus_tag="pgaptmp_002511"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HGU4429346.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLVRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21680..22351
                     /gene="nfi"
                     /locus_tag="ACHKYO_RS12560"
                     /old_locus_tag="pgaptmp_002512"
     CDS             21680..22351
                     /gene="nfi"
                     /locus_tag="ACHKYO_RS12560"
                     /old_locus_tag="pgaptmp_002512"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HGU4429347.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22394..22984
                     /gene="yjaG"
                     /locus_tag="ACHKYO_RS12565"
                     /old_locus_tag="pgaptmp_002513"
     CDS             22394..22984
                     /gene="yjaG"
                     /locus_tag="ACHKYO_RS12565"
                     /old_locus_tag="pgaptmp_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HGU4429348.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23171..23443
                     /gene="hupA"
                     /locus_tag="ACHKYO_RS12570"
                     /old_locus_tag="pgaptmp_002514"
     CDS             23171..23443
                     /gene="hupA"
                     /locus_tag="ACHKYO_RS12570"
                     /old_locus_tag="pgaptmp_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HGU4429349.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23456..24151
                     /gene="yjaH"
                     /locus_tag="ACHKYO_RS12575"
                     /old_locus_tag="pgaptmp_002515"
     CDS             23456..24151
                     /gene="yjaH"
                     /locus_tag="ACHKYO_RS12575"
                     /old_locus_tag="pgaptmp_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HGU4429350.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24153..24578)
                     /gene="zraP"
                     /locus_tag="ACHKYO_RS12580"
                     /old_locus_tag="pgaptmp_002516"
     CDS             complement(24153..24578)
                     /gene="zraP"
                     /locus_tag="ACHKYO_RS12580"
                     /old_locus_tag="pgaptmp_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HGU4429351.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            complement(24581..24709)
                     /locus_tag="ACHKYO_RS12585"
                     /old_locus_tag="pgaptmp_002517"
     CDS             complement(24581..24709)
                     /locus_tag="ACHKYO_RS12585"
                     /old_locus_tag="pgaptmp_002517"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429352.1"
                     /translation="MTRYPQQASHFYSFIMPDYPSYQRFIVGTEDAIPEVRQPLED"
     gene            24816..26192
                     /gene="zraS"
                     /locus_tag="ACHKYO_RS12590"
                     /old_locus_tag="pgaptmp_002518"
     CDS             24816..26192
                     /gene="zraS"
                     /locus_tag="ACHKYO_RS12590"
                     /old_locus_tag="pgaptmp_002518"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HGU4429353.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26189..27514
                     /gene="zraR"
                     /locus_tag="ACHKYO_RS12595"
                     /old_locus_tag="pgaptmp_002519"
     CDS             26189..27514
                     /gene="zraR"
                     /locus_tag="ACHKYO_RS12595"
                     /old_locus_tag="pgaptmp_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HGU4429354.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27511..28800)
                     /gene="purD"
                     /locus_tag="ACHKYO_RS12600"
                     /old_locus_tag="pgaptmp_002520"
     CDS             complement(27511..28800)
                     /gene="purD"
                     /locus_tag="ACHKYO_RS12600"
                     /old_locus_tag="pgaptmp_002520"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGU4429355.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTNIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28812..30401)
                     /gene="purH"
                     /locus_tag="ACHKYO_RS12605"
                     /old_locus_tag="pgaptmp_002521"
     CDS             complement(28812..30401)
                     /gene="purH"
                     /locus_tag="ACHKYO_RS12605"
                     /old_locus_tag="pgaptmp_002521"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGU4429356.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7250 a         7985 c         8425 g         7124 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agactccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc actgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgta tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg ggccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc gggttaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gggcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgtt ctgggtctgt actatatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgcaaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgtgatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtta aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca agggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaatactg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagta ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggtca gaccattact cgtcagacag acgaattgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atggggggag tttagggaaa gagcatttgt
    12541 cagaatattt aaggaatttc tgaatactca taatcaatgt agagatgact aatatcctga
    12601 aactgactga actaattgag tcaaactcgg caaggattcg atactattcc tgtgtaactt
    12661 tcttaaggaa cgagaatgaa acaggaagtg gaaaagtggc gaccttttgg acatccggat
    12721 ggtgatattc gtgatttatc atttcttgat gctcatcagg ctgtctacgt tcagcatcat
    12781 gagggcaaag agcctttaga gtatcgcttt tgggttacct actctcttca ctgcttcaca
    12841 aaagattatg aacatcagac gaacgaagaa aaacaatcgt taatgtacca cgcgcctaaa
    12901 gaatctcgtc ccttctgcca gcaccgttat aacttagcgc gcacacactt aaaaagaact
    12961 attttggcgc tgccagaaag caacgttatt catgccgggt atggtagcta tgccgtgatt
    13021 gaggtggact tagacggagg agataaggca ttttactttg ttgcgttcag ggctttcagg
    13081 gaaaagaaaa aactccgttt gcatgtaact agcgcttatc ccatttctga aaaacagaaa
    13141 ggtaaatcag tgaaattttt caccattgcc tacaacttat tgagaaataa gcagcttcct
    13201 cagccctcaa aataacaaaa cccaccttaa ggtgggtttc gccagagaat tatctctggt
    13261 attcagaacg ccattaccgg actttgcctt gaccttgcga taatcgcagg ttgcgggatg
    13321 tctgaatttc ttcagtctgc tgcatcctgg aagatgagaa catgtgttct tattttcgtc
    13381 tctatcatag ttgagtattt actctcttac aatcagatct ctttcattgc tcaacaggcg
    13441 atggcttcag actttgcatt acggaatttt taagaaaggc agggcgaaac gaggaagaag
    13501 cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca gaacgctttc
    13561 aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt tcttacattc
    13621 cgtctcatag tctttgcgag gcccgtccca gatagctgtc ccagtctttc cataccggct
    13681 gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg
    13741 agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt ttcgagaagg
    13801 cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt gattcccgcg
    13861 tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta
    13921 actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg cgcaggcgcg
    13981 gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc aaatgttcgg
    14041 caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg ccgaggccta
    14101 tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag aagaagtcct
    14161 gtttttttcc tttcagatga tggcgggcat aagtcgcctc gtgatatgtc tcctgataaa
    14221 ccatcacgcc atccagacca agctgcttta actcggcgta ttccgtctcc gccagcggtt
    14281 gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga cgacgaaagt
    14341 aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt tcaaagccca
    14401 tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt ttgcgcttga
    14461 tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat
    14521 aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga cgctgcgccc
    14581 gttgggccag ttgttccaga tagccactgg cggctggcga taacagcgcc atcatatcgt
    14641 cgcgggtgag ttgcgaggca tttagcgccc gttctacgtc agcagccgtt ttgccgttga
    14701 tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg atcgctgaag gttttcatgc
    14761 cgatgcctcc agaaatccag tcagcgggct ggtggcatga gcaaaatgac tgcggctgcc
    14821 cggtccggac tgacgtgcca gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat
    14881 gttgacggga tcgtccgcga cggcaattgc cgtattcact aacaccgcgt cggcccccat
    14941 ttccagcgcc tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa ccaccggcac
    15001 tgtggcctgc tggataataa tctccagcat ggcgcgggtt tccagtccct gattcgagcc
    15061 aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac gtttacacaa
    15121 taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca gcgtttcggc
    15181 ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt gaatctctaa
    15241 ttttaaccag tttgtgccta acgcttcacg agccagatgg gcggcgaaaa tggcttcttc
    15301 cgctgttttc gccccggatg tatttggcag cagggtcaca cccgccgcga taagcggttc
    15361 gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg
    15421 gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc
    15481 ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatttcaa ccccctgcaa
    15541 taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgcc cactgctcac
    15601 gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct
    15661 ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg ttaaacagga
    15721 tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc
    15781 cactggctcg atttaccgtc gaacagtcgg agttctcccg caggtgtctc tataccgctt
    15841 aataacttaa tggcttccag tgcctgcaaa gtgcccataa ccccgaccac cgggccaacc
    15901 acgcccgccg tgcggcagtt gcgttctggc tcctggttat ctggccacag gcagcggtaa
    15961 cacccctgct cccagggcgg cgtcagtacc atcaactgac cgccaaatcc gaccgcgctg
    16021 gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg gcgagtcgcc
    16081 atattgtcgg tacagtcgag caccacatcg gcccgtgcaa ccgcatcttt taacgcctca
    16141 cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc
    16201 tgttggctga cctgcgattt cgggcgatcg atatcttcag tggtaaagag gatttgtcgt
    16261 tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc gacgccagcg
    16321 cccgccaggt acagcgcagc aggtgtaccc agcccgccca gaccgataat cagcacctgg
    16381 ctgtcgagca gtttttgctg cccgtccaga gcgatatcgt cgagcaggat ttggcggcta
    16441 taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca actgtgccgt
    16501 tgccaaacgc cagtctgcag cttgagtaat ggcgctgacg acggcgatac tgccgacacc
    16561 cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca cggtgggata
    16621 atccgccagt cgctcaacat gccgtgccag ctgttccagc ccctgcggtg cagaaggcat
    16681 ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc gtgctgccag
    16741 cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg
    16801 gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc catacgcctg
    16861 atgcttgatc gccagccgcc agtaatcgtt gataaacaat cgcgcgttat agcggcggcc
    16921 cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat ctttgatgcg
    16981 tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta cgctgtccac
    17041 caccgggtac agtcctgaac gaaaaggtac aagaggaaaa tcaggctgat acatcacgct
    17101 tcctccttac gcaggtagat ttctccgcct ctggcacgga agttctccga catatccgcc
    17161 attcccattt caatagtttg cgtggcggcg taatcacgca cttcctggct gattttcatc
    17221 gagcagaatt tcggcccaca catggagcaa aaatgggcga ctttacctga ctcttgcggc
    17281 agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt aaactggtct
    17341 tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg cgcgcccgga
    17401 tgccctttcg ccaggtcagc ggcgtgggcg gcaatcttat aggtgataag cccctgctta
    17461 acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca gagcatcgcg
    17521 cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc atagcccggc
    17581 gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca gtgctctaac
    17641 tcctcggtca tattgcggcg gatcatctgc atcggcacgt ggcctgggcc ttcaatcatc
    17701 acctgcacgt catattccca ggcaattttg gtcagttcgc ccagcgtatg cagctcggca
    17761 aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc gcccagcgac
    17821 agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg atagaggaaa
    17881 ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg agagacgata
    17941 ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc atggatagtg
    18001 aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta
    18061 agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac tgtaccgatc
    18121 ggcaccgggc tgttacgcaa aatccactcg cgggtttcgt gaatatagcg accggtggag
    18181 agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc cacttcttct
    18241 tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac caggaaattg
    18301 cgaccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat aatcgcacgt
    18361 ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag acgtgcgcca
    18421 aagctcattc ccggatgctg gtggcgtaaa acttcgctac ggatgcgctc gcggcccata
    18481 ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc
    18541 agttgggtga cacggcgtcc tgctttggcg cgttttggtg ttagtacgcc gctaaaacgc
    18601 agttcgtcga ggccatcatc tgccagccgc gctttagtgt aatcggaact gcgcacggta
    18661 agttcttcgg tatcgccgcg cgcatcgatc cacggctggc gtagttttgc cagcccttgc
    18721 tgcacgttaa tggcaatctg cggatcacca tacgggccgg aggtgtcgta gaccggaatc
    18781 gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt cgggctaagc
    18841 tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt
    18901 gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt
    18961 tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag gacgtggctt
    19021 gtcagacgac ggatgaagca agagacgatc gccgcagggg cgatgcgata gcagattaac
    19081 tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa taaacggtct
    19141 cagccagtta aggcactccg acaagaaatt agccccgcaa atggggcatt gaatgtaaat
    19201 tacgcgttaa cagcgcagaa ctcaagcagg atgtttgacg cgggcggcgt caagcaccag
    19261 cagaatcagc ttatcttcca gcacaaagcg cgcttccagc gcctcgccga tgtcagataa
    19321 aacctgttga aactcaaggt aattatcatg atcgatggcg gtttccagac tggaatcgta
    19381 gtaatccata atctgttgag tattggcttc gagttgcggc caaatcttcg cggcgcgcgc
    19441 gagttgcccg ttgccttcca gcttatgaag aatgcgctca taaatactga aatgtccggc
    19501 agacaagtaa tcgaccaggc tctgacaaaa atcatcaagg gctttttcgt ttagcctcat
    19561 gtacgattct ttgccaggct taatgccaac cagattgtag taagccacga gcagatgctt
    19621 acgtacatgt agccagcgat caaccagttt gttacttcct ctgacgcgct ccgtcaggtt
    19681 atcgagctgg ttaagcatga ttgactccgc aagtttgtat tcaaaaactg ctcagtgaga
    19741 aatgtaaaaa ccatgttaaa catgccagtg atgcaaaggt agtgcaagag ctatggatcg
    19801 tataattgaa aaattagatc acggctggtg ggtcgtcagc catgaacaaa aattatggtt
    19861 gccgaaggga gaattgccat atggcgaagc ggcaaatttc gatcttgtgg gtcagcgcgc
    19921 actacaaatc ggcgaatggc agggggaacc tgtttggtta gtacaacagc agcggcgtca
    19981 cgatatgggg tcggtacgtc aggtcattga tctcgatgtt gggctgtttc aactggccgg
    20041 acgaggcgta caactggcgg agttttaccg atcgcataaa tactgtggtt actgcgggca
    20101 tgaaatgtat ccgagcaaaa ccgaatgggc gatgctgtgc agccattgcc gtgagcgtta
    20161 ctacccgcaa atcgccccct gcattattgt tgccatccgt cgcgatgatt cgatcctcct
    20221 cgcccagcat acccgccatc gtaacggtgt ccatacagta cttgccggat tcgtcgaagt
    20281 gggcgaaacc ctcgagcagg cagtcgcgcg ggaagtgatg gaagagagcg gaattaaagt
    20341 taaaaacttg cgttacgtga cttctcagcc gtggccgttt cctcagtctt taatgaccgc
    20401 gtttatggcg gaatatgaca gcggcgacat cgtgatcgac ccgaaagaat tgctcgaggc
    20461 gaactggtat cgctatgacg atttgccgtt actcccgccg cccggcaccg tagcgcgccg
    20521 tctgatagaa gatacggtgg cgatgtgtcg ggcagagtat gagtgatgat acactgaccg
    20581 cctgacgcac taaggaacag ccaaaatgac cgaacttaaa aacgatcgtt atctgcgggc
    20641 gctgctgcgc cagcccgttg atgtcactcc agtatggatg atgcgccagg cgggtcgcta
    20701 tctaccggaa tataaagcca cgcgcgccca ggcgggcgat tttatgtcgc tgtgcaaaaa
    20761 cgccgagctg gcgtgcgaag tgactttgca accgctgcgt cgctacccgc tggatgcggc
    20821 gatcctcttt tccgatatcc tcaccgtgcc ggacgcgatg gggttagggc tctattttga
    20881 agccggagaa ggtccgcgtt ttacctcgcc agtcacctgc aaagccgacg tcgataaact
    20941 gccaattccg gacccggaag atgagctggg ttacgtgatg aacgcggtgc gtaccattcg
    21001 tcgcgaactg aaaggcgaag tgccgctgat tggtttttcc ggcagcccgt ggacgctggc
    21061 gacctacatg gtggaaggcg gcagcagcaa agccttcacc gtgatcaaaa aaatgatgta
    21121 tgccgatccg caggcgctgc acgctctgct cgataaactg gcgaaaagcg tcactttgta
    21181 tctgaatgcg cagattaaag ccggtgctca ggcagtgatg attttcgaca cctggggcgg
    21241 cgtgcttacc gggcgcgatt atcaacagtt ctcgctctat tacatgcata aaattgttga
    21301 tggtttagtg cgtgaaaacg acggtcgccg cgtaccggtc acgctgttta ccaaaggcgg
    21361 cggacagtgg ctggaagcca tggcagaaac cggttgcgat gcgctgggcc tcgactggac
    21421 aacggacatc gccgatgcgc gccgccgtgt gggcaataaa gtcgcgttgc agggtaatat
    21481 ggatccgtcg atgctgtacg cgccgcctgc ccgcattgaa gaagaagtag cgactatact
    21541 tgcaggtttc ggtcacggcg aaggtcatgt ctttaacctt ggtcacggca ttcatcagga
    21601 tgtgccgcca gaacatgctg gcgtgttcgt ggaggcagtg catcgactgt ctgaacagta
    21661 tcaccgctaa ggagtgatta tggatctcgc gtcattacgc gctcaacaaa tcgaactggc
    21721 ttcttctgtg atccgcgagg atcgactcga taaagatcca ccggatctga tcgccggagc
    21781 cgatgtcggg tttgagcagg gcggagaagt gacgcgagcg gcgatggtgc tgctgaaata
    21841 tccctcgctt gagctggtgg agtataaagt tgcccgcatc gccaccacca tgccttacat
    21901 tccaggtttt ctttccttcc gcgaatatcc tgcgctgctg gcagcgtggg agatgctgtc
    21961 gcaaaagccg gatttagtgt ttgtcgatgg tcatgggatc tcgcatcctc gccgtcttgg
    22021 cgtcgccagc cattttggct tattggtgga tgtgccgacc attggcgtgg cgaaaaaacg
    22081 tctctgcggt aaattcgaac cgctctccag cgaaccgggc gcgctggccc cgctgatgga
    22141 taaaggcgag cagctggcct gggtctggcg cagcaaagcg cgctgtaacc cgttgtttat
    22201 cgctaccggc catcgggtca gcgtggacag cgcgctggcg tgggtacaac gctgcatgaa
    22261 aggctatcgt ctgccggagc caacgcgctg ggcggacgcg gtggcctcgg aacgtccggc
    22321 gttcgtgcgc tatacagcaa atcagcccta attcaggtaa actgcggata atttccgtat
    22381 ttgagaactc aacatgttac aaaacccaat tcatctgcgt ctggagcgcc tggaaagctg
    22441 gcagcatgtc actttcatgg cttgcttatg cgaacgcatg taccccaatt acgccatgtt
    22501 ctgccagcaa accggttttg gtgatgggca aatttaccgt cgtattctcg atctcatctg
    22561 ggaaacgctg accgttaaag acgcaaaagt aaatttcgac agccaactgg agaaatttga
    22621 agaagcgatt ccttcagccg acgatttcga tctgtacggc gtttatccgg caatcgatgc
    22681 ctgcgtggcg ttaagtgaac tggtccattc gcgtttgagt ggtgaaacgc tcgaacacgc
    22741 ggtggaagtg agtaagacct ccatcacgac cgttgcgatg ctggaaatga ctcaggctgg
    22801 tcgcgaaatg agcgatgaag agcttaaaga aaacccagct gtagagcaag aatgggacat
    22861 tcagtgggaa atattccgac ttttagctga gtgcgaagaa cgcgatatcg agctgataaa
    22921 aggccttagg gcagacctgc gtgaggcggg tgagagcaat attggtataa tttttcagca
    22981 ataagaccag aaaacgtgat ttaacgcctg atttgtcgta cctggagtct tccctttcgc
    23041 cccccgtctg gtctacattt ggggggcgaa aaaaagtggc tatcggtgcg tgtatgcagg
    23101 agagtgctat tctggcattt ccgtcgcact cgatgcttag caagcgataa acacattgta
    23161 aggataactt atgaacaaga ctcaactgat tgatgtaatt gcagagaaag cagaactgtc
    23221 caaaacccag gctaaagctg ctctggagtc cactctggct gcaattactg agtctctgaa
    23281 agaaggcgat gctgtacaac tggttggttt cggtaccttc aaagtgaacc accgcgctga
    23341 gcgtactggc cgcaacccgc agaccggtaa agaaatcaaa attgccgcag ctaacgtacc
    23401 ggcatttgtt tctggcaagg cactgaaaga cgcagttaag taagattgcg tggcagtgaa
    23461 cagttttaac gaaggggtgg tttcacccct tttgtctttc tggcgtcgat cattgatgct
    23521 ggcaggtgcg ctgtttctca ctgcctgtag ccacaactct tcacttcctc cctttaccgc
    23581 cagtggattt gctgaagacc agggcgcggt acgcatctgg cgaaaagaca gcggcgataa
    23641 tgtgcatctg cttgccgtgt ttagcccgtg gcgcagtggc gataccacga cgcgagagta
    23701 tcgctggcag ggcgataacc tcacgctcat caatatcaat gtttacagca aaccgccggt
    23761 gaacattcgc gcgcgttttg acgatcgcgg tgatctgagc tttatgcaac gtgaatccga
    23821 tggggaaaag cagcagcttt ctaacgacca aatcgattta taccgttatc gtgctgctca
    23881 gatccgccag attagcgatg ccttacgtca ggggagagtc gtgctgcgcc aggggcgctg
    23941 gcatgcgatg gaacagaccg tgaccacctg cgaagggcaa accattaaac ctgatttaga
    24001 ttcgcaggcg atagcgcata tcgagcgccg ccagagccgc tcttctgttg atgtcagcgt
    24061 ggcatggctg gaagcgcccg aaggttcgca attactgtta gtggcaaact ctgatttctg
    24121 tcgctggcaa cccaacgaga aaacgttctg atttaccagt ggcccatacc catatgaccg
    24181 ccaccaccgc agccgccgta gcccatgccc attccggctc cgcgcggaat acccgcttca
    24241 gccatcgcga tatcccgttt cacccgtaac tcatctaacg actgacgcaa attctccatc
    24301 tctttggcga ccgcgttaat tttgctgcta tccggtgggt tcgcggctaa cagggcattg
    24361 tattcataac gcttcgtcac cagttgctgt tgcagtgcgc tgctttgagc gtaaaagtca
    24421 ttatggattt tctgccacgc tgtctgttgt tcgctggtca aaggcgcggc attttgctgc
    24481 cacataccgt gtccgccgtg agcaaatgca gatgtcgatc ccatcgccat tgctgaaagc
    24541 gccatcatta ccagggcaat tttcgtgttc cgtttcatgg ttaatcctcc agtggttgtc
    24601 ttacttcggg tattgcatct tccgtgccaa cgatgaaacg ctgatatgac gggtaatctg
    24661 gcatgataaa cgagtaaaaa tgactcgcct gctgcgggta gcgagtcatt tttactcatt
    24721 gaaacttaag cctttgtgtt acagcgcagg gtaagcgctg ataaaagatg gcatgatttc
    24781 tgctgtcaga aagggatgag caggcaaaga agaagatgcg ttttatgcaa cgttctaaag
    24841 actccttagc taaatggtta agcgcgatcc tccccgtggt cattgttggg ctggtagggt
    24901 tgtttgcggt gacggtgatt cgcgattatg ggcgcgagac tgccgccgcc agacaaacgc
    24961 tgctggaaaa aggcagtgta cttattcgcg ctcttgaatc cggctcgcgc gtcggcatgg
    25021 ggatgcgcat gcatcatgcg cagcagcagg ccttactgga agaaatggcc gggcagcctg
    25081 gagtacgttg gtttgcggtc acggatgaac aaggaacaat cgtgatgcat agcaactccg
    25141 gcatggtggg aaaacagctt tattccccgc aggaaatgca gcagttacat ccgggagatg
    25201 aagaagcgtg gcggcggatc gatagcgcag acggcgagcc tgttctggaa atttatcgcc
    25261 agtttcaacc gatgtttgct gctggaatgc accggatgcg ccatatgcag cagtatgccg
    25321 cgacaccaca agcaattttc atcgctttcg acgccagtaa tattgtgagt gccgaagatc
    25381 gtgagcagag aaacaccctg attatcctct tcgccctggc gacggtcttg ctggcaagcg
    25441 tattgtcatt cttctggtat cgccgctatc tgcgctcgcg ccagcttcta caagatgaaa
    25501 tgaagcgcaa agagaagcta gtggcgctgg ggcatcttgc ggcaggcgtt gcccacgaaa
    25561 tccgtaaccc actttcctcg attaaaggac tggcgaaata ctttgccgag cgcgcgcctg
    25621 cagggggaga agcgcatcaa ctggcgcagg tgatggcgaa agaggccgac cgtttaaacc
    25681 gcgtggtaag cgagctgctg gaactggtta agccaacgca tctggctttg caggcggtgg
    25741 atctcaacac gctgattaac cactcattac agctggtagg ccaggatgca aacagccggg
    25801 agatccagtt acgctttacc gccaacgaca cattaccgga aattcaggcc gatccggaca
    25861 ggctgactca ggtcctgttg aatctctatc tcaatgctat tcaggcgatt ggtcagcatg
    25921 gcgtgattag cgtgacggtc agcgaaagcg gcgcgggcgt gaaaatcagc gttaccgaca
    25981 gcggtaaggg aattgcggca gatcagcttg aagccatctt cactccgtac ttcaccacca
    26041 aagccgaagg caccggattg gggctggcgg tcgtgcataa tattgttgaa caacacggtg
    26101 gtacaattca ggtcgcaagc caggagggaa aaggctcaac gttcaccctc tggcttccgg
    26161 tcaatattac gcgtaaggac ccacaaggat gacgcacgat aatatcgata ttctggtggt
    26221 ggatgatgac attagccact gcactatttt gcaggcttta ctgcgcggct ggggctataa
    26281 cgtcgcgctg gcgaacagcg ggcgacaggc gctggagcag gtgcgggagc gggtttttga
    26341 tcttgtgctt tgcgatgtgc gaatggcaga gatggacggc attgccacgc taaaagagat
    26401 caaagcgtta aatccggcga ttccggtact gattatgacc gcatactcca gcgtcgagac
    26461 ggcggtagag gcactgaaaa ccggggcgct ggattatctc atcaagccgc tggatttcga
    26521 caccctgcag gcgacgctgg aaaaggcgct cgcgcatacg cacagtattg atgctgaaac
    26581 gcctgcggtg actgccagcc agttcggtat ggtcggtaaa agcccggcga tgcaacacct
    26641 gctcagtgaa atcgccctcg tcgcgccatc ggaagccacg gtgctgatcc acggcgattc
    26701 cggcaccggt aaagagctgg tcgccagggc gattcacgcc agtagcgcac gtagcgaaaa
    26761 accgctggta acgctcaact gtgcggcact caacgaatcc ttgctggaat ctgaattgtt
    26821 cggtcacgaa aaaggggcgt ttactggagc cgataaacgc cgggaagggc gctttgttga
    26881 ggcggacggc ggcacgctgt ttctcgatga aattggcgat atctcgccga tgatgcaggt
    26941 gcgtctgctg cgtgcgattc aggagcgcga agttcagcgt gtcggtagca accagactat
    27001 ctcggttgat gtccggctga ttgcggcgac ccatcgcgat cttgccgcag aggtgaatgc
    27061 cgggcgtttt cgccaggatc tctactatcg cctgaatgtg gtggcgattg aagtaccatc
    27121 gctgcggcaa cggcgggagg atatccctct gctggctagc cattttcttc agcgttttgc
    27181 cgagcgtaat cgcaaggcgg taaaaggctt tacgccacag gcgatggatt tgctgatcca
    27241 ctacgactgg ccgggaaaca ttcgtgaact ggaaaacgcg gtggaaaggt cggtggtgct
    27301 gctgaccggg gaatatattt ccgaacgcga gctgccgctg gcgattgcca gtacgccgat
    27361 cccgctgggg caaagtcagg atattcagcc gctggtggaa gtcgaaaaag aagtgattct
    27421 ggcggcactg gagaaaacgg gcggcaacaa aaccgaagcc gcccgtcagt tagggatcac
    27481 gcgcaaaacg ctattggcaa aactgtcgcg ttagttctgc tcgcgttcga tagcgcgcca
    27541 gccgatatct ttccggcaga agcagtcgtc ccagtgaata ttggtcatta aggcataagc
    27601 gcgtttctgc gcttctgcca cggtatgacc cagcgcggtg acgcacagta cgcgcccgcc
    27661 gctggtcact acctgctcgt tatccgccag tttcgtgccc gcgtggaaca ctttgccgcc
    27721 tgccacttct tccagcggca ggccgtggat cacgtcaccg gtgcggtaat cacccggata
    27781 tccacccgca gccatcacca cgccgagaga agcgcgttca tcccactctg acgttttctc
    27841 gtccagtttg ccttcacagg ccgccaggca gagctcaacc agatcggact tcatgcgcag
    27901 cataatcggc tgggtttctg gatcgccaaa gcggcagtta aattcgataa ccttcggatt
    27961 gccctgtttg tcgatcatca ggcccgcgta gagaaaaccg gtgtaggtgt tgccttccgc
    28021 cgccatgcct ttcacggttg gccagatgat acgttccatg gtgcgctgat gaacttcatc
    28081 ggttactacc ggcgcggggg agtaagcgcc catcccgccc gtgtttggtc cggtatcttt
    28141 atcgcctacg cgtttgtgat cctggctggt ggccatcggc agcacatgct cgccgtctac
    28201 catcacgata aagctcgctt cttcgccatc gaggaactct tcgataacga tgcgatgacc
    28261 cgcgtcgcca aaagcgttgc ccgccagcat atcgtgaaca gccgcttccg cttcttccag
    28321 cgtcatcgcc acgataacgc ctttcccggc agccagaccg tccgctttaa tgacgattgg
    28381 cgcgcctttc tcacgcagat acgccagcgc aggttctacc tcggtgaagt tctggtattc
    28441 cgccgtaggg atcttatggc gggccaggaa atctttggta aacgcttttg agccttccag
    28501 ttgggccgca cctgcggttg ggccgaagat tttcagcccg gcggcgcgga aggtatcgac
    28561 cacgcctttc accagcggcg cttccgggcc gacgatggtc agatcaatct tttcgttttg
    28621 tgcgaaatcc aacagcgccg ggatatcggt cacgccaata gcaacgtttt gcagcgcggg
    28681 ttccagtgca gtgcctgcat tacccggagc aacaaaaaca gtctcaacca gcggcgactg
    28741 ggccgctttc caggccagcg cgtgctcgcg cccgccgtta ccaatcacta atactttcat
    28801 ctattgctcc attaatggcg gaagtggcgc atgtcggtga agagcatcgc aataccgtgc
    28861 tcgtcggcgg cggcaatcac ttcgtcatca cggatggaac cgccaggctg gattacgcag
    28921 gtcacgcccg cagcggcggc ggcatcaata ccgtcgcgga acgggaagaa cgcgtcagaa
    28981 gccatcgagg aacctttcac ttccaggcct tcatcggcag ctttaatacc ggcgattttc
    29041 gcggagtaca cgcggctcat ctggcccgcg ccaataccga tggtcatatt gtttttggca
    29101 tagacgatag cattggattt cacgaacttc gccactttcc agcagaacag cgcatcgcgc
    29161 agttcctgtt cggtcggctg acgttgggtg acgacgcgca gttcttccgc accgaccatc
    29221 cccaggtcac gatcctgaac cagcagaccg ccgttcacgc gtttgaaatc aagacccgga
    29281 acacgctcgc cccactgacc gcaggtcaga acgcgtacgt tctgcttggc ggcggtgatt
    29341 ttcagggctt cttcgctggc ggaaggcgca ataatcactt cgacaaactg acgagaaatg
    29401 atggcctgtg cggtttccgc atctagctcg cggttaaagg caatgatgcc gccgaatgcg
    29461 gaggttgggt cggttttgta cgcgcgatcg taagcatcaa gaatggaatt gccgatagct
    29521 acaccgcaag ggttggcgtg cttcacaatc acacatgccg gctcggcgaa ctctttcacg
    29581 cactccagcg ccgcatcggt atcggcgatg ttgttataag agagggcttt accctgaacc
    29641 tgggttgcgg tagcaacgga ggcttctttc acattctctt ctatatagaa ggcagcctgc
    29701 tggtggctgt tctcgccgta acgcatatcc tgcttcttaa tgaagttcag gttcagcgtg
    29761 cgtgggaagc gaccagcggc ttctttgctt tcaccgtggt acgccggaac catgctgccg
    29821 aagtagttgg caatcatgct gtcgtaggcg gcagtgtgtt cgaaggcttt gatggcgagg
    29881 tcgaaacggg ttgcaagcgt cagcgatcct tcgttgtcat ccatctcttt aataatggcg
    29941 tcgtagtcgc tgctcttcac cacgattgcg acatctttat ggttcttagc ggcggagcgc
    30001 accatggtcg ggccgccgat atcgatgttc tcaaccgcat cttccagcga gcaaccttca
    30061 cgggccacgg tctgggcgaa cggatacagg ttaacaacca ccatatcgat aggctggatc
    30121 tgatgttctt ccataatggc atcgtcctgg ccgcgacggc ccagaatgcc accatgtact
    30181 ttcggatgca gggtcttcac gcgtccatcc atcatctccg ggaaaccggt gtaatcggaa
    30241 acttcggtta ccggcagacc tttttctgct aacagacggg cagtgccccc tgtagacagc
    30301 agctccacac cgcgtgcgga aagtgcctgg gcgaattcga cgataccggc tttgtcagaa
    30361 acactgagca gagcgcggcg gactggacga cgttgttgca tggtaaatcc cctggatttg
    30421 actattacag agagcgttag ctgaattttt cgcgaaaaac tcagctaacg cccctaacgg
    30481 ggcatcctta tttttcgccc gcattgtaac gaaaacgttt gcgcaacgct cgcgaatttt
    30541 tctctttcaa tggtgatcac aattttgaca gtggttaccg tgggaaaaat acagaaatta
    30601 cattgatgat tgtggataac tctgtgcgta aaaaggtata aagcggggtt ttgctgggga
    30661 atgcagcagt cagtcatttt tctgcaattt ttctattgcg gcctgcggag aactccctat
    30721 aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc ggttcggttg
    30781 aaga
//
LOCUS       DAZGAN020000050        30104 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGAN020000050 DAZGAN020000000
VERSION     DAZGAN020000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.050"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..2131)
                     /locus_tag="ACHKYO_RS12610"
                     /old_locus_tag="pgaptmp_002522"
     CDS             complement(<1..2131)
                     /locus_tag="ACHKYO_RS12610"
                     /old_locus_tag="pgaptmp_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HGU4429357.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGSLPPADPAVTAAA
                     GAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHA"
     gene            complement(2146..2466)
                     /locus_tag="ACHKYO_RS12615"
                     /old_locus_tag="pgaptmp_002523"
     CDS             complement(2146..2466)
                     /locus_tag="ACHKYO_RS12615"
                     /old_locus_tag="pgaptmp_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HGU4429358.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            complement(2602..3057)
                     /locus_tag="ACHKYO_RS12620"
                     /old_locus_tag="pgaptmp_002524"
     CDS             complement(2602..3057)
                     /locus_tag="ACHKYO_RS12620"
                     /old_locus_tag="pgaptmp_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429359.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQDTVPVSRAAPVAREWREIPRTEPDPETTPDNSYRNYL"
     gene            complement(3050..3337)
                     /locus_tag="ACHKYO_RS12625"
                     /old_locus_tag="pgaptmp_002525"
     CDS             complement(3050..3337)
                     /locus_tag="ACHKYO_RS12625"
                     /old_locus_tag="pgaptmp_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HGU4429360.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            complement(3330..3929)
                     /locus_tag="ACHKYO_RS12630"
                     /old_locus_tag="pgaptmp_002526"
     CDS             complement(3330..3929)
                     /locus_tag="ACHKYO_RS12630"
                     /old_locus_tag="pgaptmp_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4429361.1"
                     /translation="MMMPALQKVPFSGLPFSGICGYSFSAVAKSAAGRGNPSNLTATY
                     DAPCVFFCVAINATERQIMVWCVVSRADGTPCILPASAHYAAESMVAQAGQPPGWPVS
                     VRAGISTPVWAIAIERGNSGDSVTCYLTEAAIMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            complement(3926..4192)
                     /locus_tag="ACHKYO_RS12635"
                     /old_locus_tag="pgaptmp_002527"
     CDS             complement(3926..4192)
                     /locus_tag="ACHKYO_RS12635"
                     /old_locus_tag="pgaptmp_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4429362.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            4744..5478
                     /locus_tag="ACHKYO_RS12640"
                     /old_locus_tag="pgaptmp_002528"
     CDS             4744..5478
                     /locus_tag="ACHKYO_RS12640"
                     /old_locus_tag="pgaptmp_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HGU4429363.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPDKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAVLKARGLLS"
     gene            5475..5975
                     /locus_tag="ACHKYO_RS12645"
                     /old_locus_tag="pgaptmp_002529"
     CDS             5475..5975
                     /locus_tag="ACHKYO_RS12645"
                     /old_locus_tag="pgaptmp_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HGU4429364.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAERLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            6049..6621
                     /locus_tag="ACHKYO_RS12650"
                     /old_locus_tag="pgaptmp_002530"
     CDS             6049..6621
                     /locus_tag="ACHKYO_RS12650"
                     /old_locus_tag="pgaptmp_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HGU4429365.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDVA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            7032..7433
                     /locus_tag="ACHKYO_RS12655"
                     /old_locus_tag="pgaptmp_002531"
     CDS             7032..7433
                     /locus_tag="ACHKYO_RS12655"
                     /old_locus_tag="pgaptmp_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein gop"
                     /protein_id="HGU4429366.1"
                     /translation="MLNHVYGLIGVVGTIITIATSFIRNQDISQWLLVCSGWLAALLI
                     GWFTHRTIKAISNNHTNVIKSNMEVIKSHNESNQNLMAENKELIKELARTSEQKEKME
                     SIAAYLATQNPQINAMPRTASRPENIDSEAN"
     gene            7436..8503
                     /locus_tag="ACHKYO_RS12660"
                     /old_locus_tag="pgaptmp_002532"
     CDS             7436..8503
                     /locus_tag="ACHKYO_RS12660"
                     /old_locus_tag="pgaptmp_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HGU4429367.1"
                     /translation="MKVYFENYSYYPALRTRSAEMTGLNNLSYENKKKILPLISLGKW
                     PRSEEIQVSLDKSLEVMSNLPFILDVTKDNSHHCASSFELLSPENGFKNWIEFCSRND
                     NIIPVVQMPDSAKLRDISIQARVLEELKGSIAFRIRNLNTDINKTLTSLVSMNSPENT
                     IVFIDLGYIRGNVSAITAAAINSINQIRTEIPEAIISVLATSFPSSVTNFCRENGQSG
                     YIDVIERELHQNIGGSDVAIYGDHGSIHSVVYDNIIGRYVPRIDIALNDSWYFERRPG
                     MNKEGFIEAAKSILAEYPHYQREDSWGAAMIRNAAIGDIAGMGSPAKWIAVRVNLHLN
                     KQIELSEALQYGFDHDEEDLI"
     gene            complement(8531..9325)
                     /locus_tag="ACHKYO_RS12665"
                     /old_locus_tag="pgaptmp_002533"
     CDS             complement(8531..9325)
                     /locus_tag="ACHKYO_RS12665"
                     /old_locus_tag="pgaptmp_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HGU4429368.1"
                     /translation="MAELLREQEVKIALIEWLYKKGMLSDATIINEMVVANWSRRADL
                     AIANGSLQAFEIKSDFDSLKRLDGQLKIFKSSFEKVTIVCAPKFTSEVSKRVSSDVGV
                     VEFQSNNSSVRFKIIQKGRVCSRLNKFVYLGFLLKSELKDLLNQYGITFSNELHRDCL
                     EVLASKLSLNKIREFVITSIKNRYHATSNDFLSRLHGNQEISINDLALLSKAKRRNNS
                     LVKFEFKACSEKSLNEYHKVDVDSIVRKYGDDVSYIPEKVLKRVVG"
     gene            complement(9432..10700)
                     /locus_tag="ACHKYO_RS12670"
                     /old_locus_tag="pgaptmp_002534"
     CDS             complement(9432..10700)
                     /locus_tag="ACHKYO_RS12670"
                     /old_locus_tag="pgaptmp_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4429369.1"
                     /translation="MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDEAKKLLVQGIDPKVVQKEARAENSGAYTFEA
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMILLNQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MMQWWADYLDMNRNKYITPYDYSKYKEILK"
     gene            complement(10864..10948)
                     /locus_tag="ACHKYO_RS12675"
                     /old_locus_tag="pgaptmp_002535"
     tRNA            complement(10864..10948)
                     /locus_tag="ACHKYO_RS12675"
                     /old_locus_tag="pgaptmp_002535"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10912..10914),aa:Leu,seq:caa)
     gene            11144..12163
                     /gene="ahr"
                     /locus_tag="ACHKYO_RS12680"
                     /old_locus_tag="pgaptmp_002536"
     CDS             11144..12163
                     /gene="ahr"
                     /locus_tag="ACHKYO_RS12680"
                     /old_locus_tag="pgaptmp_002536"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HGU4429370.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRVDWQWVIPLPENIDIKSAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQELLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            complement(12167..12730)
                     /gene="idnK"
                     /locus_tag="ACHKYO_RS12685"
                     /old_locus_tag="pgaptmp_002537"
     CDS             complement(12167..12730)
                     /gene="idnK"
                     /locus_tag="ACHKYO_RS12685"
                     /old_locus_tag="pgaptmp_002537"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HGU4429371.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKHYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            12947..13978
                     /gene="idnD"
                     /locus_tag="ACHKYO_RS12690"
                     /old_locus_tag="pgaptmp_002538"
     CDS             12947..13978
                     /gene="idnD"
                     /locus_tag="ACHKYO_RS12690"
                     /old_locus_tag="pgaptmp_002538"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HGU4429372.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            14002..14766
                     /gene="idnO"
                     /locus_tag="ACHKYO_RS12695"
                     /old_locus_tag="pgaptmp_002539"
     CDS             14002..14766
                     /gene="idnO"
                     /locus_tag="ACHKYO_RS12695"
                     /old_locus_tag="pgaptmp_002539"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HGU4429373.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            14831..16150
                     /gene="idnT"
                     /locus_tag="ACHKYO_RS12700"
                     /old_locus_tag="pgaptmp_002540"
     CDS             14831..16150
                     /gene="idnT"
                     /locus_tag="ACHKYO_RS12700"
                     /old_locus_tag="pgaptmp_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HGU4429374.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYVNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            16217..17215
                     /gene="idnR"
                     /locus_tag="ACHKYO_RS12705"
                     /old_locus_tag="pgaptmp_002541"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             16217..17215
                     /gene="idnR"
                     /locus_tag="ACHKYO_RS12705"
                     /old_locus_tag="pgaptmp_002541"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HGU4429375.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYQGNTL"
     gene            17293..18795
                     /gene="yjgR"
                     /locus_tag="ACHKYO_RS12710"
                     /old_locus_tag="pgaptmp_002542"
     CDS             17293..18795
                     /gene="yjgR"
                     /locus_tag="ACHKYO_RS12710"
                     /old_locus_tag="pgaptmp_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HGU4429376.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGNNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDVKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGVQASAEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(18914..19996)
                     /gene="lptG"
                     /locus_tag="ACHKYO_RS12715"
                     /old_locus_tag="pgaptmp_002543"
     CDS             complement(18914..19996)
                     /gene="lptG"
                     /locus_tag="ACHKYO_RS12715"
                     /old_locus_tag="pgaptmp_002543"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU4429377.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(19996..21096)
                     /gene="lptF"
                     /locus_tag="ACHKYO_RS12720"
                     /old_locus_tag="pgaptmp_002544"
     CDS             complement(19996..21096)
                     /gene="lptF"
                     /locus_tag="ACHKYO_RS12720"
                     /old_locus_tag="pgaptmp_002544"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU4429378.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(21159..21209)
                     /gene="ytgA"
                     /locus_tag="ACHKYO_RS12725"
                     /old_locus_tag="pgaptmp_002545"
     CDS             complement(21159..21209)
                     /gene="ytgA"
                     /locus_tag="ACHKYO_RS12725"
                     /old_locus_tag="pgaptmp_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HGU4429379.1"
                     /translation="MSIIILATISTTQENK"
     gene            21363..22874
                     /gene="pepA"
                     /locus_tag="ACHKYO_RS12730"
                     /old_locus_tag="pgaptmp_002546"
     CDS             21363..22874
                     /gene="pepA"
                     /locus_tag="ACHKYO_RS12730"
                     /old_locus_tag="pgaptmp_002546"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HGU4429380.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            23008..23451
                     /gene="holC"
                     /locus_tag="ACHKYO_RS12735"
                     /old_locus_tag="pgaptmp_002547"
     CDS             23008..23451
                     /gene="holC"
                     /locus_tag="ACHKYO_RS12735"
                     /old_locus_tag="pgaptmp_002547"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HGU4429381.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            23451..26306
                     /gene="valS"
                     /locus_tag="ACHKYO_RS12740"
                     /old_locus_tag="pgaptmp_002548"
     CDS             23451..26306
                     /gene="valS"
                     /locus_tag="ACHKYO_RS12740"
                     /old_locus_tag="pgaptmp_002548"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGU4429382.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYRETLDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(26362..27558)
                     /gene="yjgN"
                     /locus_tag="ACHKYO_RS12745"
                     /old_locus_tag="pgaptmp_002549"
     CDS             complement(26362..27558)
                     /gene="yjgN"
                     /locus_tag="ACHKYO_RS12745"
                     /old_locus_tag="pgaptmp_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HGU4429383.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKLWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            27751..28254
                     /gene="yjgM"
                     /locus_tag="ACHKYO_RS12750"
                     /old_locus_tag="pgaptmp_002550"
     CDS             27751..28254
                     /gene="yjgM"
                     /locus_tag="ACHKYO_RS12750"
                     /old_locus_tag="pgaptmp_002550"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4429384.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEFDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHINYALGCTGHVDCE
                     VRMLREL"
     gene            complement(28300..28716)
                     /gene="rraB"
                     /locus_tag="ACHKYO_RS12755"
                     /old_locus_tag="pgaptmp_002551"
     CDS             complement(28300..28716)
                     /gene="rraB"
                     /locus_tag="ACHKYO_RS12755"
                     /old_locus_tag="pgaptmp_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HGU4429385.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            28878..29882
                     /gene="argF"
                     /locus_tag="ACHKYO_RS12760"
                     /old_locus_tag="pgaptmp_002552"
     CDS             28878..29882
                     /gene="argF"
                     /locus_tag="ACHKYO_RS12760"
                     /old_locus_tag="pgaptmp_002552"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGU4429386.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT         7750 a         7437 c         7654 g         7263 t
ORIGIN      
        1 aggcgtgata gaggtattta cggtaattac gcgggatgat actggcgttc cccatataca
       61 tgccgctggt ctgcggcagg gtttccagat agccgataaa atcaaacgtc gggtcggcat
      121 cccgtttgat gttcagtgcc tcgtctgagt tctgctggga ctgaagcagt gaccgggcga
      181 gcatcgggtc gctgaacttc tgcatcaggt gacgcacgat gaccgccagc tcgcgggtga
      241 ttttgtcctt aagttgcggg tcgcgctcct gcggggctat ctgttccggg aagtgaataa
      301 tcacccgccg gcgtgacacg ccgccgctgc ggtcggtgaa gcgcatcggg ttattgttca
      361 cggccagaat caccgccggg atatgcgtgg agtacgcatc ccggtatttc gggtccacgg
      421 acaccgcatc gccgccggtg atggccttga gtccggcacc gtcgccgctc catttttcct
      481 ggtccggcag gcgtatcagt gagaagccag ttaacgcggc acgttcacgc ggggattcca
      541 gcgtctcaat ggtggccgac gtggcgttat cctccccggc cagcagggtg gctatttcgg
      601 ccatgatact tttgccgctg ccgccgggac cggtcacctc cagaaagagc tgccagtcat
      661 agcggtttgc cagcaccata aacagtgcgg ccagaatcac gtcgcgtttt tccgcacggc
      721 caccggcggc acggtcaagc cagcgccaga acgcgggggc gtgggtttcc agcgtttccc
      781 cttccaccgg cggggtgaaa tccacatcgc acagggtgcg catccagtgt gacggactgt
      841 gcgggtggaa cgtgccgttc tgcgtgtcga gcacgccgtt acgaaagcca atcaggcggc
      901 gggagggggc ttcctgctgc ggaataatca gcttcagggt gtccaccacg gaggccacct
      961 tcccggagga gaacggcgcg cgcagacgct gaaacagccc ggccacatcc cgggcaaagt
     1021 cctgtggcgg cagcaccttc cagacaccat tttcataacg ggacagaagc tggccgttgg
     1081 catcaaccgc gagcgcctcg ccgtaatgct catagatacg catggccttt tcgctggtac
     1141 tcatggcgga aaactccgct tcgctcatgg tatcgaacgg gctttcagcc ggtggccgga
     1201 tggcatcgta aatggcctta cgggtggctt ccccgccgta ctgcgtgaag gcatcattcc
     1261 agtcaccgaa gaccggcggc agggcaacaa caccttcaca cgcatctgcg gctgcggcgg
     1321 cttttttctg gccgtcacca ctgaggtcac ggtcagcggc aaggacaatc tgacaggcgg
     1381 ggtgcttctg ccgggcaagg ctggccagag aaaggaggtt cacggaagaa agcgccacca
     1441 tcaccgtttc accggtcagg tgatgtacgg taagtgcggt cgcgtatccc tccgctatcc
     1501 acagacgttt tccggcctga ttctgtcctt caagggtgtg acaggtgccc ctgacctgtc
     1561 cgcctttcag ggtgcgctta cggccgtcag cactgattaa ctgaaggtta accagttcgc
     1621 cgctgtcgtc atacagtggc accacaaggt caccggcgcg ccagctcacg ccaccggctc
     1681 tgtgtgtgcc ggtcagcatc cggcattccc ggccgggaaa gcccttgcgg gtcaggtagg
     1741 cgttaccggt tccgggacgg gtttttgcca tcagggtttg tgccagtgcg gcggcgttct
     1801 tccgggcagc gtctgtttca gcgccggcgg cggccgtcac tgccgggtca gccgggggca
     1861 ggctgccggt cacggcagcc acctttgcgg ccgcgtcgga cggggaaaca ccaaacacct
     1921 tttcaaccag tttcaggccg tcaccggcac cacactgatt gcagtaccag gtgccgcgcc
     1981 cctccctgtc atcaaaacgg aaacggtcac tcccgccaca gaccggacag ggctgatgac
     2041 ggtttttcag cacctgaatc cccagcgccg ggagaatacg cggccagtgg ccgagtgcat
     2101 ggctgacggt ggcggttacg ttcattttca tggtgttgtt ctccttcagt gcagtaccgg
     2161 cgcttttatg tgacgggcac agagttcatc catcacaacc agcccgagaa aggacagcga
     2221 cggcgcggcc ttcagggggc cggattccat taaatcttcc agcagggcac aggctatctg
     2281 acgccctttt tcctcaccgt gctggcgcag ataaaaacct tccagctcag cggcgatggc
     2341 cgcctccagt gactcaaggg tgagatgcgg gtagcggtgc tgacgttcgc acacggtcag
     2401 ccaggcacag gcgacagcgc gacggtaaag ggcagcgcgt aagacgggcg gtaagggtgt
     2461 tttcatttgc tttcctccct gtgacagatg actgcattcc gtgccggttg cattaactga
     2521 taaggcatat ctgcgtctcc tgaagacgtg cgtatccctg cgcgaatacg cacatttaat
     2581 ttttcgggtg tcgtttttta attacagata attgcggtaa ctgttatccg gggtggtttc
     2641 cgggtcaggc tccgtgcggg gaatttcccg ccattcccgc gccaccggtg ctgcccggct
     2701 gaccggaaca gtgtcctgcg ggtaaatatc cagatatttc tcccgccatt tctgtaattc
     2761 cgggtctccg gccatttctt tcagtaccgc atgccggttt acggggctgc gtttaaacag
     2821 gtcaggacgg tcacaggtaa attcccgcag aaaacgcccc agcgggatgt ctgtggtgcg
     2881 cccgtcggcg aggatacgca caaggatact gaatttacgg cggtacggat tccagacaat
     2941 gtccgggcag cggtacggca tttcccacgg aataccgtct tccagaatgc cgaccacggc
     3001 cacatcggga aaaccggcag aacggtaaat ctcaccgggc tggggaaaat caaacatgcg
     3061 tcctgtctcc ccggtctttc tgctgggcga gaaaatcgcg gcacaggcct ttggctttca
     3121 gctcattcag cacaaaatca atatcttcat tcaggtagct gaaaatatgc ggaatgtaga
     3181 gctgatgcag gccggagagt tcacggtgaa tcaaatcacc cccaacaaac tgggatacgg
     3241 cgctggcgcg gttgagctta tggtaagcct caatgctgag gtgttcacgg gcgtcatgac
     3301 gcgctgagac ggtctgaggg gcttttttat tacgcacggg acacctccac caccggcaga
     3361 cgggcagcaa gggagagcac atagtcacgg acaagggaac ggcgggcact gcgttcatca
     3421 ccggcgacgg tgcgaagcat gcagatacgg ggatgacggt ctgcgcgacg gacagccgca
     3481 aacacaaaga caaattcagg gtgtgaaggg gtaagggttg tagccatgat ggcagcctcc
     3541 gttagatagc aggttacgct atcgccggag tttccacgct cgatggcgat agcccagacg
     3601 ggggtggaaa taccggctct aacggatacc ggccagcccg gaggctgccc cgcctgagct
     3661 accattgact ctgcggcata atgagcggac gcgggcagga tgcacggagt gccatctgct
     3721 cgactgacca cacaccacac cataatctgg cgctctgtgg cgttgattgc gacacaaaaa
     3781 aagacgcatg gcgcgtcata tgtcgccgtt agattgctcg ggtttccacg cccggctgcc
     3841 gattttgcga cagcggaaaa actatatccg caaatgccgg aaaaaggcaa gccagaaaaa
     3901 gggacttttt gcagagcggg catcatcatg cgtcgtaccc ccgtttgcgt ccggcaatgc
     3961 gtccggccat ccatgcggtg acttcagagt gcagccaggc cacattttta ccgccaagac
     4021 tcacctgcgg cggaaattcc cccttacgga tgagttcata gatggtcgag cgtgacaggc
     4081 cgcacaggtg catcacttcc ggcagacgta aaaaacgctc ctgcgtgatg tccggcagcg
     4141 gcatcagtgg cgtcacaggg gcgggagacg gggaagaaaa aacagcttgc atcgggctac
     4201 ctcgttaatg tccatacagc accggataag tccgtccggc ttcgggtagc gctttatttt
     4261 gtgaatattt ttggcagacg caacaggggg gatttgttcc ggcagcctta caatggctgt
     4321 gcattttttg tacatcagcg ccagatagct ttaaaacgct ctggaaggag ctggaaaaaa
     4381 ttatagtgaa atacaaattg ttttttctta tttatttcag tgaattaata aaaataaaaa
     4441 gtaataaaca gcacaaaaag cccatcaacg ggtgaacagt ggtgaacaga cggtgaacag
     4501 tcattactgc gattgttcac cctttaactt attgtattac ttatcttttt tcttatggtg
     4561 aacagaggtg aacagtaaaa tataaaaaaa caaacagtaa gccggttttt cctgcgacct
     4621 tttcctggct tgccggtctg aggatgagtc tcctgtgtca gggctggcac atctgcaatg
     4681 cgtcgtgttg ttgtccggtg tacgtcacaa ttttctcaac ctgaagtgac gaggagccgg
     4741 aaaatgtctg acaacaccat ccctgaatat ctgcaacccg cgctggcaca actggaaaag
     4801 gccagagccg cccaccttga gaacgcccgc ctgatggatg agaccgtcac ggccattgaa
     4861 cgggcagagc aggaaaaaaa tgcgctggcg caggccgacg gaaacgacgc tgacgactgg
     4921 cgcacggcct ttcgtgcagc cggtggtgtc ctgagcgacg agctgaaaca gcgccacatt
     4981 gagcgcgtgg cacgccggga gctggtacag gaatatgaca atctggccgt ggtgctgaat
     5041 ttcgaacgtg aacgcctgaa aggggcgtgt gacagcacgg ccaccgccta ccggaaggca
     5101 catcatcacc tgctgagtct gtatgcagag catgagctgg aacacgccct gaatgaaacc
     5161 tgtgaggcgc ttgtccgggc aatgcatctg agcattctgg tacaggaaaa tccgctcgcc
     5221 aacaccaccg ggcatcaggg ctacgtcgca cccgataaag ctgtcatgca gcaggtgaaa
     5281 tcatcgctgg aacagaaaat aaaacagatg caaatcagcc tcaccggcga gccggttctc
     5341 cggctgaccg gactgtcagc ggcaacactc ccacacatgg attatgaggt ggcaggcaca
     5401 ccggcacagc gcaaggtgtg gcaggacaaa atagaccagc agggagcagt gcttaaggcc
     5461 agaggactgc tgtcatgatt tactgcccgt cgtgtggaca tgttgctcac acccgtcgcg
     5521 cacatttcat ggacgatggc accaagataa tgattgcaca gtgccggaat atttattgct
     5581 ctgcgacatt tgaagcgagt gaaagctttt tctctgactg taaagattca ggaatggaat
     5641 acatttcagg caaacagaga taccgcgatt cactgacgtc agcctccggt agcatgaaac
     5701 gcccgaaaag aatgcttgtt accggatatt gttgtcggag atgtaaaggc cttgcactgt
     5761 caagaacatc gcggcgtctg tctcaggaag tcaccgagcg tttttatgtg tgcacggatc
     5821 cgggctgtgg tctggtgttt aaaacgcttc agaccatcaa ccgcttcatt gtccgcccgg
     5881 tcacaccgga cgaactggca gaacgcctgc atgaaaaaca ggaactgccg ccagtacggt
     5941 taaaaacaca atcatattcg ctgcgtctgg aatgagggct accggttaac cccggccgtc
     6001 gccgcacacc gtatttttat tcttcagcat gatgagaaag agataacgat ggaaagcaca
     6061 gccttacagc aggcctttga cacctgtcag aataacaaag cagcatggct gcaacgcaaa
     6121 aatgagctgg cagcggccga acaggaatat ctgcggcttc tgtcaggaga aggcagaaat
     6181 gtcagccgcc tggacgaatt acgcaatatt atcgaagtca gaaaatggca ggtgaatcag
     6241 gccgccggtc gttatattcg ttcgcatgaa gccgttcagc acatcagcat ccgcgaccgg
     6301 ctgaatgatt ttatgcagca gcacggcaca gcactggcgg ccgcactggc accggagctg
     6361 atgggctaca gtgagctgac ggccattgcc cgaaactgtg ccatacagcg tgccacagat
     6421 gccctgcgtg aagcccttct gtcctggctt gcgaagggtg aaaaaattaa ttattccgca
     6481 caggatagcg acattttaac gaccatcgga ttcaggcctg acgtggcttc ggtggatgac
     6541 agccgtgaaa aattcacccc tgcgcagaac atgatttttt cgcgtaaaag tgcgcaactg
     6601 gcatcacgtc agtcagtgta aaattccccg aaaatccgcc cgtttttact gaaaaaagcc
     6661 atgcatcgat aaggtgcatg gctttgcatg cgttttcctg cctcattttc tgcaaaccgc
     6721 gccattcccg gcgcggtctg agcgtgtcag tgcaactgca ttaaaaccgc cccgcaaagc
     6781 gggcgggcga ggcggggaaa gcaccgcgcg caaaccgaca agttagttaa ttatttgtgt
     6841 agtcaaagtg ccttcagtac atacctcgtt aatacattgg agcataatga agaaaatcta
     6901 tggcctatgg tccaaaactg tcttttttga tggcactatc ctgaaaaata tgcaaaaaat
     6961 agattgatgt aaggtggttc ttatcagtgt cgcaagatcc ttaagaattc gtggcatgag
     7021 agagttaaag gatgctgaat catgtatatg gattaattgg cgttgttggg accataatca
     7081 ctattgctac tagcttcatc cgcaaccaag atatttcaca atggttgctc gtttgttctg
     7141 gatggttagc tgctttactt attggatggt ttacacacag aacgattaaa gcaattagca
     7201 acaaccacac gaatgttata aaaagtaata tggaggtaat caagagtcat aatgaatcca
     7261 atcaaaactt gatggctgaa aacaaagaat tgattaaaga acttgcaaga acttcagaac
     7321 agaaagagaa gatggaaagt atagctgctt atcttgctac tcagaacccc caaattaacg
     7381 ctatgccaag aacagcaagc cgaccagaaa atatcgattc ggaggctaat taaatatgaa
     7441 agtatatttt gaaaattact catactatcc agccctaaga acacgatcag cagaaatgac
     7501 cggactgaat aatttatcat atgagaataa gaaaaaaata ttacctttaa tttctttggg
     7561 gaaatggcct cgctcggaag aaatacaggt atcgttagat aaaagtttag aagtgatgag
     7621 taatctccca tttattttgg atgtcacaaa ggataattct catcattgcg cgtcaagctt
     7681 cgaactactc tctcctgaga atggttttaa aaactggata gagttttgtt ccagaaatga
     7741 taatattatt ccagtagtcc agatgccgga ttcagcaaaa cttagagata tatctatcca
     7801 agccagggtt ttagaggaac ttaaaggttc aattgcattt agaattagaa atctaaatac
     7861 agacattaat aaaaccctga cgtcactagt atcgatgaac tctccagaaa ataccatagt
     7921 tttcatagat ttgggctaca ttagagggaa tgtatctgct ataacagctg cagcaataaa
     7981 ttcgattaat cagattagaa ctgagattcc tgaagccata attagtgtgc tagcaacaag
     8041 cttccctagt tcagttacaa atttttgccg tgaaaatgga cagtccggat atattgatgt
     8101 tattgagaga gaattgcatc aaaatattgg tggaagtgat gtcgctattt atggcgatca
     8161 tggttcaatt cattcagtag tgtatgacaa cataattggg cgatatgttc ctagaattga
     8221 tatagcgtta aatgattcat ggtatttcga gcgccgccct gggatgaata aagaagggtt
     8281 cattgaagct gcgaaatcaa ttttggcaga gtatccacat tatcaaaggg aggactcttg
     8341 gggagctgca atgataagaa atgctgctat aggggatata gcaggtatgg gaagtccagc
     8401 taaatggatt gctgttcgag tgaatctaca cttaaataag caaattgagc tatctgaagc
     8461 tctacagtat ggctttgatc atgatgagga agatcttatc taataaaaaa ctagggaagg
     8521 ttagccttcc ctagcccaca acccttttaa gtactttttc cggaatatag gaaacatcat
     8581 cgccatattt cctaactata gagtcaacat caaccttatg gtattcattt aaagatttct
     8641 ccgaacaagc cttaaactca aacttaacca aagaattatt ccttctttta gctttgctca
     8701 ataaggcaag atcgtttata gatatttctt gattaccatg gagtctagat aaaaaatcat
     8761 tagatgtcgc atgataccta tttttaatag atgtaatgac aaattctcta atcttgttta
     8821 aagataactt agatgccaaa acctcaagac aatccctatg tagttcattt gaaaatgtaa
     8881 ttccatactg attaagcaga tctttaagct cagacttaag caaaaaaccc aaataaacaa
     8941 atttatttaa gcgagagcaa acccttcctt tttgaattat tttaaagcgt acgctactat
     9001 tattagattg aaattctaca acgccaacat ctgatgaaac ccgtttagat acttcactgg
     9061 taaattttgg cgcgcacaca attgtaactt tttcgaagct agatttaaaa atttttaact
     9121 gaccatccaa gcgcttaaga gagtcaaaat cgctttttat ttcaaaagct tgtaggcttc
     9181 cattggcaat agccaaatca gctcttctgc tccagttagc aacaaccatt tcattaatga
     9241 tcgttgcatc actaagcatg ccttttttat ataaccattc aattagggct attttaactt
     9301 cctgctctcg caataactca gccatgttaa tcacactcac ttcacgttga tttttattat
     9361 agcaactaac aattaaaaac tccagattat gttacttaac actcatttta aaaatcaacc
     9421 aataactatt tttatttaag tatttctttg tattttgaat aatcataagg cgtaatatat
     9481 ttattacggt tcatatccaa ataatcagcc caccactgca tcattaaacg ccgctcatcc
     9541 aaatgctcgg aggtatggat atatgccgca cgtacattat tacgctctga gtggctcagt
     9601 tgcctctcta tcgcgtcatc actccataac cccgactccc ccaacgcacc acgcgccata
     9661 gtcctaaacc catgcccaca cacctcggtt ttagtatcat agcccatcgc acgcaatgcg
     9721 ctgtttaccg tgttttcact cataacctta gttgcgtcat gatcccccgg aaaaagcagc
     9781 tctttatcac cactaatctg ctttaactgg tttaataaaa tcatcgcctg tcgactaagc
     9841 ggaacgatat gttcctcttt catcttcatg cctcggtacg aataacgcac acctttaatt
     9901 tcttctcgct ttgcaggtat acgccaaaga gatttatcga agtcgaattc atcccaacgt
     9961 gcgaaacgta attcactgga acgcacaaaa gttagcaagg aaagctcgac cgcaatccgt
    10021 gtcattacac ggccacgata tgcagcaaga cgtgcaagaa actcagggaa tcggctagaa
    10081 ggtaaagcag ggtaatgtcg cgctttggtt gtcgatagcg caccagccat atcgctggct
    10141 ggatttgagt cgatgtaatc gttctgtacg gcataacgca taatggctgt gacgcgctgt
    10201 tgcaggcgct gagcgacatc gtgtttgcca ctggcatcaa cttttttaat cggggctaac
    10261 aggtggctgg ttttaagctg ccgaatgtcg gacgaaccga tatgagggaa tatataaagc
    10321 tcaagatagc gaagaacgcg cgatcgatgg tcttcactcc agcgcttgtt actggcatgc
    10381 cattctcggg cgatagcttc aaaagtatat gcccccgaat tctcggcccg agcttctttt
    10441 tgtacgactt ttgggtctat gccctgtact aaaagctttt tagcttcatc gcgttttgct
    10501 cttgcctgag caagcgtcac agtaggccaa acaccaaacg caagacgatc ctcttttttg
    10561 tcagagggac gtctgtattt catgcgccag tatttggaac ctttggccga aacctcaaga
    10621 tacaaaccac caccatcggc cattttgtag gttttgtctt ttggctttgc ggtctcgacc
    10681 tgtctggcgt tgagcttcat ttgggggcac atttctaatc gaagttaaga tgcccccaat
    10741 tatgccccca atgatatccg gatttcaacg gacaacctcg gaagacgcag gacgaaaaaa
    10801 tcgctgcaag cattgatttt aaagggatat ttggactttc tcggatggtc ttggaagtaa
    10861 gaatggtgcc gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa
    10921 ctgcgtctac cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt
    10981 atacctgtcg cacgccacca tgcaagcgcc agcttgctcg catctcgcta agtgctgaaa
    11041 ttttcagcat tgcgtacagc gatgtgtaac ctttgtcaca ctccatgcac cccgccctgc
    11101 catgctctac acttcccaga ccacaccaga gaaggaccaa aaaatgtcaa tgataaaaag
    11161 ctatgccgca aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct
    11221 gaagccacaa gatgttgaag tgcaggtgga ttactgcggg atctgccatt ccgatctgtc
    11281 gatgatcgat aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat
    11341 tggtcgcgtg gtggcgctcg ggagtgccgc gcaggataaa ggtttgcagg tcggtcagcg
    11401 tgtcgggatt ggctggacag cgcgtagctg tggtcactgc gacgcctgta ttagcggaaa
    11461 tcagatcaac tgtgagcaag gtgcggtgcc aacaattatg aatcgcggag gttttgccga
    11521 gaagttgcgt gtagactggc aatgggttat tccactgccg gaaaatatcg acattaaatc
    11581 tgccgggccg ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat
    11641 cactgctacc agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa
    11701 acttctgcac gcaatgggat gtgaggtgac ggcctttagt tctaatccgg cgaaagagca
    11761 ggaattgctg gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa
    11821 agcactggcg gggcagtttg atctcattat caataccgtg aacgtcagcc tcgactggca
    11881 gccttatttt gaggcgctga cgtacggcgg taatttccac actgtcggtg cggttctcac
    11941 gccgctgtct gttccggcct ttacgttaat tgcgggcgat cgcagcgtct ctggctctgc
    12001 taccggcacg ccttatgaac tgcgtaagct gatgcgcttt gccgcccgca gcaaggttgc
    12061 gccgacaacc gaactgttcc cgatgtcgaa aattaacgac gccatccagc atgtgcgcga
    12121 cggtaaggcg cgttaccgcg tggtgttgaa agccgatttt tgaaaatcat tcgcagcgct
    12181 gatctgaggc gctgccctct ttcgcacata ttctgttttg tcgtatcgcc agcacagcct
    12241 gccgacattg ttcggtgaca ttggcaatat catggttgat atcaatgcgc acaatatcct
    12301 gttcatctgc ttgtggacgc tccagcgcct caaactgact ttttagtaac gctaccggca
    12361 taaaatgccc agcccgacgc tgcattcgcg cgaggatagt ttcatagtcg ccatctaacc
    12421 agaggaaatg aacatggggg ctacccttgc gtaaaatatc acgataatgt ttttttaatg
    12481 atgaacagac aataaatcct gtttcattct ttttataaag actgtatgaa gcatcattta
    12541 agcgttccag ccagggaagt cgatcttcat cagataatgg aataccctgc gacattttat
    12601 ctatattttt ggctggatga agatcgtcac catcaataaa tttagcagat aataacgcgg
    12661 caaccttgct accaattaat gttttaccac tccctgaaac gcccatcaaa ataaagcttt
    12721 cacccgccat ttaaaatctc ctgaatcatc ttacctgctg cgatactacg cctggtggca
    12781 taataatttc acgttttttt atttctgctg ataagaatta caaggcacat cacgttatgc
    12841 gtaacatagt aatgtaacaa ttttctgacg tgatcttcat cacaaataat gacaattaaa
    12901 accgcttaaa tgcttccgag gtgtctacct gaccagtgag gaatttatgc aagtgaaaac
    12961 acagtcctgc gttgttgcgg gcaagaaaac tgttgccgtt accgagcaga cgatagattg
    13021 gaataataat ggaacattag tacaaataac ccgaggtgga atttgcggtt ccgatttaca
    13081 ttattatcag gaaggaaaag taggtaattt catgataaag gcaccgatgg tgttaggtca
    13141 tgaagttatc ggtaaagtta ttcatagcga ctcatcagaa ttacatgaag ggcaaacggt
    13201 agccattaat ccgtctaaac cgtgcggtca ctgcaaatac tgcattgaac ataacgagaa
    13261 tcagtgtaca gagatgcgtt tttttggcag tgccatgtat ttccctcatg ttgatggtgg
    13321 ttttacccgt tataaaatgg tcgaaacgtc gcaatgtgtc ccttatccgg ccaaagctga
    13381 cgaaaaggtt atggcttttg ccgaaccttt agccgtcgcg attcatgccg cacatcaggc
    13441 cggcgagtta cagggcaagc gagtatttat ttccggtgtt ggacccattg gctgcctgat
    13501 tgtcagtgca gtgaaaacac tgggggccgc ggaaattgtc tgtgctgatg tgagtccccg
    13561 ttccctttcg ctgggcaaag agatgggggc ggatgtgctc gtaaacccac aaaacgacga
    13621 catggatcac tggaaagcgg aaaaaggcta tttcgatgtc agctttgaag tgtccggtca
    13681 tccttcatca gtgaatacct gtctggaggt cactcgtgca cgcggcgtaa tggtgcaggt
    13741 aggtatggga ggcgcgatgg cagaattccc aatgatgacg ttgattggta aggagatttc
    13801 actcagaggc tctttccgtt ttaccagcga atttaatacc gcagtgtcat ggctggcgaa
    13861 tggcgttatc aatccactgc ctttactgag tgctgaatat cccttcactg acctggaaga
    13921 ggcgctacgt ttcgccggtg ataaaaccca ggcagcaaaa gtccagcttg ttttctaaga
    13981 gataaataaa ggaataatac aatgaacgat ctattttcac tggcaggaaa aaatatcttg
    14041 attaccggtt cagcacaggg cattggcttt ttactggcaa ccggcctggg taaatatggc
    14101 gcacaaataa ttattaatga tattactgcc gaacgcgcag aacttgctgt agaaaaactc
    14161 caccaggagg gtattcaggc cgttgccgca ccttttaatg ttactcataa acatgaaatt
    14221 gatgccgccg ttgaacatat cgaaaaggac atcggcccca ttgatgtgct ggtgaataac
    14281 gccggtatcc agcgccgtca tccttttact gagttccctg aacaagagtg gaatgatgtg
    14341 atcgcagtaa accagaccgc cgtgttcctg gtatcgcaag cggtaactcg tcacatggtt
    14401 gaacgcaagg caggtaaagt tattaatatt tgctcgatgc aaagcgaact gggacgtgac
    14461 accatcaccc cttatgccgc atcgaaaggg gcggtaaaaa tgctcacccg cggcatgtgc
    14521 gtcgagctgg cgcgccacaa tattcaggtc aacggtattg cgccgggcta tttcaaaaca
    14581 gaaatgacta aagcactggt tgaggacgaa gccttcaccg cctggttgtg caaacggacc
    14641 cccgccgcac gctggggaga tccgcaggaa ctgattggtg ctgcggtgtt cctttcttca
    14701 aaagcctctg atttcgtaaa cggccacctg ttgtttgttg atggcggcat gttagtggct
    14761 gtttaacacc tactacccta cgtgcagcgc gaaagcgctg cttttctggc actaataaga
    14821 gataaagact atgccattaa tcattattgc ggcaggcgtc gcgctgcttc ttatcctgat
    14881 gatcggcttt aaagttaacg gctttattgc cctcgttctg gtagctgccg tcgtcggatt
    14941 tgccgaaggg atggatgcac aggccgtcct gcactctata caaaatggta tcggcagcac
    15001 gctcggcggg ctggcaatga ttctcggttt cggggccatg ttaggcaagc tgatttctga
    15061 tacgggtgcg gcacaacgta tcgccactac gctgattgct acttttggta aaaaacgcgt
    15121 gcaatgggcg ctagtgatca ccggtctggt tgtgggcctc gccatgtttt ttgaagtggg
    15181 ttttgtcctg ctgttgccgt tggtatttac catcgtagca tcatcaggat tacccctgtt
    15241 gtatgttggc gtaccaatgg tagcagcgct ctctgtaacc cactgttttc tgccgccaca
    15301 tccagggcct actgccatcg cgactatctt tgaggctaat ctcggaacga ctttactgta
    15361 tggatttatc attaccattc cgacagttat tgtcgcagga ccgctgtttt ctaaactgct
    15421 aactcgcttt gagaaagcac caccggaagg cttatttaat cctcatctgt ttagcgaaga
    15481 ggagatgccc tccttctgga acagtatttt cgctgccgtg atcccggtca tcctgatggc
    15541 tatcgccgcc gtttgtgaaa ttacgttacc gaaaactaac accgtgcgcc tcttctttga
    15601 atttgtcggt aaccctgccg ttgcgctgtt tattgccatt gttattgcga ttttcacact
    15661 gggccgacgt aatggacgca ccatcgagca aatcatggat atcattgggg attctatagg
    15721 cgctatcgcg atgattgtgt ttattatcgc tggcggcggc gcgtttaagc aggtattagt
    15781 agatagcggt gtcgggcact atatttcaca cttaatgacc ggaactacgc tttcgccgtt
    15841 attgatgtgc tggactgttg cggcgctgtt gcgtatcgct ctgggctctg ccaccgtcgc
    15901 ggccattacc accgcgggtg tggtgttgcc gattatcaac gttacccatg ccgatcccgc
    15961 tttaatggta ctggcaaccg gtgcgggcag cgtgatcgcg tcacacgtaa acgaccctgg
    16021 cttctggcta tttaaagggt atgttaatct gacggttggt gaaacgttgc gtacctggac
    16081 ggtgatggaa acccttattt ctattatggg tttgctgggc gtgttagcca ttaacgccgt
    16141 attgcactga aagaataaaa tattgtgatg tagtttcggg ggaagattga agattccccc
    16201 gacgttggga gaagttatgc gcaatcacag aatttcttta caggatatcg ctacgctggc
    16261 tggcgtaaca aaaatgaccg tgagtcgtta tatccgctcg ccgaaaaagg tggcaaagga
    16321 aacaggcgag cgcatcgcca aaattatgga agagatcaat tatatcccta atcgcgctcc
    16381 cggcatgttg ttgaatgcac aaagttatac ccttggtatc ttgattccgt catttcaaaa
    16441 tcaactcttc gctgacattc tcgcgggaat agaatccgtc acctctgagc ataattatca
    16501 gacccttatt gctaattaca attacgatcg cgattccgaa gaagagtcgg tgattaacct
    16561 gctctcttat aacattgacg ggattatact ttcggaaaaa tatcacacca tcaggacggt
    16621 gaaatttctg cgttcagcca ccattccggt cgtggaattg atggatgtac agggagaacg
    16681 gctggatatg gaggtcggtt ttgataatcg gcaggccgct tttgacatgg tgtgtaccat
    16741 gctggagaag cgagtcaggc acaaaattct gtatctcggt tccaaagatg acacccgcga
    16801 tgaacagcgt tatcaggggt attgcgatgc gatgatgctg cataatcttt ccccattacg
    16861 catgaatcca cgcgccatct catccattca tttaggaatg cagttaatgc gtgatgccct
    16921 tagcgcaaac ccggatttag acggtgtatt ttgcaccaat gacgatattg cgatgggtgc
    16981 gcttctgctg tgccgcgagc gaaacctcgc cgtgccagaa caaatttcca ttgcgggttt
    17041 tcatgggctg gagattggca ggcaaatgat cccaagcctc gccagcgtaa ttactccccg
    17101 ttttgatatt ggtcggatgg ccgcccaaat gttgctgagc aaaattaaaa ataacgatca
    17161 caaccacaac actgttgacc tcgggtatca gatttatcaa ggcaacacgc tttaggttat
    17221 tttctcttca ttacacgtat tctctttgcc ggacgttgcc tatactccag gcaggaaact
    17281 ggaggaaatc tcatgagcga acccctgtta attgcccgca cgccggacac agaactgttt
    17341 ttactgccgg gaatggctaa ccgtcacggg ctgattactg gcgcaacggg gacgggtaaa
    17401 accgttacgc tgcaaaaact ggcggagtca ttgtcggaaa tcggcgtgcc ggtgtttatg
    17461 gctgatgtga aaggcgatct gaccggtatc gcgcaggcag gaacggcgtc ggaaaaactg
    17521 ctcgcaaggc ttaaaaatat cggcgtcaat gactggcaac cgcatgccaa tccggtggtg
    17581 gtgtgggata tctttggcga gaaaggccat ccggtgcggg cgacggtttc agacctgggg
    17641 ccgctgttgc tggcgcggct gttgaatctc aacgatgtgc aatctggcgt gctgaatatc
    17701 atcttccgca ttgctgacga tcaggggctg ttactgctcg actttaaaga tttgcgggcg
    17761 attacccagt acatcggcaa taacgccaaa tctttccaga atcagtacgg taatattagt
    17821 agcgcatcgg ttggtgccat ccagcgcggg ctgttatcgc tggaacagca aggcgcagca
    17881 cacttctttg gcgagccgat gctggatatc aaagactgga tgcgcaccga taccaacggt
    17941 aaaggcgtta tcaatatcct cagcgccgag aagctttatc agatgccgaa actgtacgcc
    18001 gccagcctgc tgtggatgct ttcagagttg tatgaacaat tgccggaagc aggcgatctg
    18061 gagaaaccaa aactggtgtt tttcttcgac gaggcacatc tgctgtttaa cgatgcaccg
    18121 caggtactgc tggataagat tgagcaggtg atacggctta ttcgctcaaa aggcgtaggc
    18181 gtctggttcg tttcgcaaaa cccgtctgat attccggata atgtgctcgg gcagctcggt
    18241 aatcgcgttc aacacgcttt gcgtgctttt acgcccaaag atcagaaagc ggtaaaagct
    18301 gcggcgcaaa ccatgcgggc caatccggca tttgataccg aaaaggcaat ccaggaactg
    18361 gggaccggcg aggcgttaat ctcgtttctt gatgtgaaag gaagtccttc agtggtggaa
    18421 cgggcgatgg tgattgcgcc ttgttcgcgg atgggaccgg tgacggaaga tgagcgtaat
    18481 ggcttgatta atcactctcc ggtgtatggc aagtacgagg atgaggtgga ccgggaatcc
    18541 gcctatgaga tgttgcaaaa aggcgtacag gcaagcgccg agcagcaaaa taatcccccc
    18601 gcgaaaggga aagaggtggc ggtggatgac ggcattcttg gtggattaaa ggatattttg
    18661 tttggcacta ccggaccacg cggcgggaag aaagatggtg tggtgcaaac aatggcgaaa
    18721 agcgccgccc gccaagtgac gaatcagatt gtacgcggga tgttggggag tttgctgggg
    18781 ggaagaagaa ggtaagtcag gtgcggtctg tgcccctcac cctaaccctc tccccaaagg
    18841 ggcgagggga ccgatcgaac tcgtatttga ctttatcaac aatgtgaaag ggagctttcg
    18901 ctcccttgct tggttacgat tttctcatta acagccacag gctgattaag aagaagctgg
    18961 cgcttggcaa cagtgcgccg atgatcggcg ggatgccata aaccaacgtc agcgggccaa
    19021 agatctggtc cagtacgtag aagacaaaac cgaagctgat accggtgacc acacgcacgc
    19081 ccatcggtac gctacgcagt gggccaaaga tgaacgacag cgccatcagc atcatcaccg
    19141 ccacggatag cggctggaag attttgctcc acatattgag ctgataacgt ccggcatcct
    19201 gaccgctcga cttcagatac ttcacgtagt tgtgcaaacc gctgattgag agtgcatccg
    19261 ggtccagcgc caccacgccc agtttgtctg gcgtgaggtt ggttttccag gtgccgctca
    19321 ccgtctgcga accggtaatc tgtttcggat tggtcaaatc agattcatca acctgcgaca
    19381 gacgccagac tttatgttcc gggtcaaact tcgcagtagc ggcatagcgt acggattgta
    19441 gacgacgatt ctcgttaaag gcataaatgc tgatgccacc taactcttcg tcacctttaa
    19501 cccgctcaat gtagacgaag ttgttgccat ctttcgccca taagccttgc tgggtagaga
    19561 gcaacgagcc gccgtacatc gcctgcgcac ggtagttacg cgccatctgc tcgccctgcg
    19621 gcgcgaccca ttcaccaatc gccatcgtca gcaagaccag cggaatggcg gttttcatca
    19681 ccgacagcgc cacctgcata cgggtaaaac cagaagcctg catcaccacc agttcgctgc
    19741 gctgcgccag catcccaaga ccaagcaacg ccccaagcag agccgccatc gggaagaaga
    19801 tttgcacatc tttcggcacg ctcagcaagg tatacattcc tgcgcctaac gcgtcgtaac
    19861 tcccctgccc ggcttttttc agctgatcga caaacttgat aatgccggac agcgacacca
    19921 gcatgaacag cgtcatcatg atggtggtga aaatagtttt accgatatag cggtcaagta
    19981 cgccaaaagg ttgcatcaca ccgctccttt acgcgaaaaa ctggcgcgca ggcggcggac
    20041 cggcacagtg tcccaaaggt tgagaacaat cgccaaagcc agataaatca ggttaacggt
    20101 ccacatccac agcgtcgggt ccagcttacc tttaccgccg ttcgatttca gggaggtctg
    20161 gatcaggaag aaaagtagat acagcagcat ggctggcagc atcgacagta cgcgtccctg
    20221 acgtgggtta accacgctca acggtacgac cataagtgcc atcataaaca cggtgaatac
    20281 caacgtgata cgccagttca gttctgcgcg ggcacgatcg gtgtcagtgt tccacaatgt
    20341 acgcatgtcc atctggtcgg tatcgttcgg gtcgagcgcc accgcctggt gaccaatgat
    20401 cgcctgataa tcctggaagt ccgtaatgcg gaaatcacgt aacagtgcag tgccttcgaa
    20461 gcgcgttccc tggttgagag tgacgacctg ggagccgtcg cgcagctggg ttaaatgtcc
    20521 ggaatcggcc accaccacag aaggacgtgc attacctttt ggtcgaattt gcgcgaggaa
    20581 cacatctttg aaatcgctgc cgtcaacgct ttcgatgaac agcaccgaac tgccattagt
    20641 cgcttgctgg aattgccctt gcgccagcgc cgccatgcca gggttcgctt tcgcttctgc
    20701 taacacttca tcctgatgac gcgatgacca cggtcccgcc cacatcacgt taaccgccgc
    20761 aacgattgcc gtgaataccg caaggatcat tgccgctttc accagaaccg ctttgctcag
    20821 gccgcaggca tgcattaccg taatttcact ttcggtatac agtttgccca gcgtcatcag
    20881 cagcccgagg aacaggctta atggcaggat aagctgcgcc atttccggca cgcccaaccc
    20941 gagaagggag agcaccagat tcgccggaat atcgccgtca accgctgcgc cgaggatcct
    21001 cactaacttt tgacagaaga agatcaaaag caagatgaag agtatcgcca gctggctttt
    21061 gagcgtctcc cgcaccagat atcttatgat tatcacttta aatacgcccg taaaaactcg
    21121 tcttttgcag gattttagct tgtttcatgg cttaaacgtc atttattctc ttgagtcgtc
    21181 gaaatcgtcg ctaagataat tatactcaac ggattcacct ctcagatttt gttctgacgt
    21241 gccaatgccg taataacgtt aagattaaca cgaagtcatc gcaacagcgg acatgagtta
    21301 cgaaagcttg caattctatc tgtagccacc gccgttgtct ttaagattca ggagcgtagt
    21361 gcatggagtt tagtgtaaaa agcggtagcc cggagaaaca gcggagtgcc tgcatcgtcg
    21421 tgggcgtctt cgaaccacgt cgcctttctc cgattgcaga acagctcgat aaaatcagcg
    21481 atgggtacat cagcgccctg ctacgtcggg gcgaactgga aggaaaaccg gggcagacat
    21541 tgttgctgca ccatgttccg aatgtacttt ccgagcgaat tctccttatt ggttgcggca
    21601 aagaacgtga gctggatgag cgtcagtaca agcaggttat tcagaaaacc attaatacgc
    21661 tgaatgatac tggctcaatg gaagcggtct gctttctgac tgaactgcac gttaaaggcc
    21721 gtaacaacta ctggaaagtg cgtcaggctg tcgaaacggc aaaagagacg ctctacagtt
    21781 tcgatcagct gaaaacgaac aagagcgaac cgcgtcgtcc gctgcgtaaa atggtgttca
    21841 acgtgccgac ccgccgtgaa ctgaccagcg gtgagcgcgc gatccagcac ggtctggcga
    21901 ttgccgccgg gattaaagca gcaaaggatc ttggcaatat gccgccgaat atctgtaacg
    21961 ccgcttacct cgcttcacaa gcgcgccagc tggctgacag ctacagcaag aatgttatca
    22021 cccgcgttat cggcgaacag cagatgaaag agctggggat gcattcctat ctggcggtcg
    22081 gtcagggttc gcaaaacgaa tcgctgatgt cggtgattga gtacaaaggc aacgcgtcgg
    22141 aagatgcacg cccaatcgtg ctggtgggta aaggtttaac cttcgactcc ggcggtatct
    22201 cgatcaagcc ttcagaaggc atggatgaga tgaagtacga tatgtgcggt gcggcagcgg
    22261 tttacggcgt gatgcggatg gtcgcggagc tacaactgcc gattaacgtt atcggcgtgt
    22321 tggcaggttg tgaaaacatg ccaggcggtc gcgcctatcg tccgggcgat gtgttaacca
    22381 ccatgtctgg tcaaaccgtt gaagtgctca acaccgacgc tgaaggccgc ctggtactgt
    22441 gcgacgtgtt aacttacgtt gagcgttttg agccggaagc ggtgattgac gtggcgacgc
    22501 tgaccggtgc ctgcgtgatc gcgctgggtc atcacattac tggtctgatg gcgaaccaca
    22561 atccgctggc ccatgaactg attgccgcgt ctgaacaatc cggtgaccgc gcatggcgct
    22621 taccgctggg tgacgagtat caggaacaac tggagtccaa ttttgccgat atggcgaaca
    22681 ttggcggtcg tcctggtggg gcgattaccg caggttgctt cctgtcacgc tttacccgta
    22741 agtacaactg ggcgcacctg gatatcgccg gtaccgcctg gcgttctggt aaagcgaaag
    22801 gcgcaaccgg tcgtccggta gcgttgctgg cacagttcct gttaaaccgc gccgggttta
    22861 acggcgaaga gtaagtttgc gttttaccct caccctaacc ctctccctaa gggagaggga
    22921 accgatcgag cgcaatagaa attcattgct catcttttag ggtgaggaat gcagcaatat
    22981 ttaaatccac cacaagaagc cccatttatg aaaaacgcga cgttctacct tctggacaat
    23041 gacaccaccg tcgatggctt aagcgccgtt gagcagctgg tgtgtgaaat tgccgcagaa
    23101 cgttggcgca gtggtaagcg cgtgctcatc gcctgtgaag atgaaaagca ggcttaccgg
    23161 ctggatgaag ccctgtgggc gcgtccggca gaaagctttg ttccgcataa tttagcggga
    23221 gaaggaccgc gcggcggtgc gccggtggag atcgcctggc cgcaaaaacg cagcagcagc
    23281 ccgcgcgata tattgattag tctgcgaaca agctttgcag attttgccac cgctttcaca
    23341 gaagtggtag acttcgttcc ttatgaagat tctctgaaac aactggcgcg cgaacgctat
    23401 aaagcctacc gcgtggctgg tttcaacctg aatacggcaa cctggaaata atggaaaaga
    23461 catataaccc acaagatatc gaacagccgc tttacgagca ctgggaaaag cagggctact
    23521 ttaagcctaa tggcgatgaa agccaggaaa gtttctgcat catgatcccg ccgccgaacg
    23581 tcaccggcag tttgcatatg ggtcacgcct tccagcaaac catcatggat accatgatcc
    23641 gctatcagcg catgcagggc aaaaacaccc tgtggcaggt cggtactgac cacgccggga
    23701 tcgctaccca gatggtcgtt gagcgcaaga ttgccgcaga agaaggtaaa acccgtcacg
    23761 actacggccg cgaagctttc atcgacaaaa tctgggaatg gaaagcggaa tctggcggca
    23821 ccattacccg tcagatgcgc cgtctcggca actccgtgga ctgggagcgt gaacgcttca
    23881 ccatggacga aggcctgtcc aatgcggtga aagaagtttt cgttcgtctg tataaagaag
    23941 acctgattta ccgtggcaaa cgcctggtaa actgggaccc gaaactgcgc accgctatct
    24001 ctgacctgga agtggaaaac cgcgaatcga aaggttcgat gtggcacatc cgctatccgc
    24061 tggctgacgg cgcgaaaacc gcagacggta aagattatct ggtggttgcg actacccgtc
    24121 cggaaaccct gctgggcgat actggcgtag ccgttaaccc ggaagatccg cgttacaaag
    24181 atctgattgg caaatatgtc attctgccgc tggttaaccg tcgtattccg atcgttggcg
    24241 acgaacacgc cgacatggaa aaaggcaccg gctgcgtgaa gatcaccccg gcgcacgact
    24301 ttaacgacta tgaagtcggt aaacgtcacg ccctgccgat gatcaacatc ctgacctttg
    24361 acggcgatat ccgtgaaagt gcccaggtgt tcgataccaa aggtaacgaa tctgacgttt
    24421 attccagcga gatcccggca gaattccaga aactggagcg ttttgctgca cgtaaagcgg
    24481 tcgttgctgc ggttgacgca ctcggcctgc tggaagaaat taaaccacat gacctgaccg
    24541 ttccttacgg cgaccgtggc ggcgtggtca tcgaaccaat gctgaccgac cagtggtacg
    24601 tgcgtgccga tgtcctggcg aaaccggcag ttgaagcggt tgagaacggc gacattcagt
    24661 tcgtaccgaa gcagtacgaa aacatgtact tctcctggat gcgcgatatt caggactggt
    24721 gtatctctcg tcagttgtgg tggggtcacc gtatcccggc atggtatgac gaagcgggta
    24781 acgtttatgt tggccgcaac gaagacgaag tgcgtaaaga aaataacctc ggtgctgatg
    24841 ttgtcctgcg tcaggacgaa gacgttctcg atacctggtt ctcttctgcg ctgtggacct
    24901 tctctaccct tggctggccg gaaaacaccg acgccctgcg tcagttccac ccaaccagcg
    24961 tgatggtatc tggtttcgac atcatcttct tctggattgc ccgcatgatc atgatgacca
    25021 tgcacttcat caaagatgaa aatggcaaac cgcaggtgcc gttccacact gtttacatga
    25081 ccggtctgat tcgtgacgac gaaggccaga agatgtccaa atccaagggt aacgttatcg
    25141 acccgctgga tatggttgac ggtatttcgc tgccagaact gctggaaaaa cgtaccggca
    25201 atatgatgca gccgcagctg gcggacaaaa tccgtaagcg caccgagaaa cagttcccga
    25261 acggcattga gccgcacggc accgatgccc tgcgcttcac cctggcggca ctggcgtcta
    25321 ccggtcgtga catcaactgg gatatgaagc gcctggaagg ttaccgtaac ttctgtaaca
    25381 agctgtggaa cgccagccgc tttgtgctga tgaacacaga aggtcaggat tgcggcttca
    25441 acggcggcga gatgacgctg tcgctggcgg atcgctggat tctggcggag ttcaaccaga
    25501 ccatcaaagc gtaccgcgaa acgctggaca gcttccgctt cgatatcgcc gcaggcattc
    25561 tgtatgagtt cacctggaac cagttctgtg actggtatct ggagctgact aagccggtaa
    25621 tgaacggtgg caccgaagcc gaactgcgcg gtactcgcca tacgctggtg accgtgctgg
    25681 aaggtctgct gcgcctcgcg catccgatca ttccgttcat caccgaaacc atctggcagc
    25741 gtgtgaaagt actttgcggt atcactgccg acaccatcat gctgcagccg ttcccgcagt
    25801 acgatgcatc tcaggttgat gaagccgcac tggccgacac agagtggctg aagcaggcga
    25861 tcgttgcggt acgtaacatc cgtgcagaaa tgaacatcgc gccgggcaaa ccgctggagc
    25921 tgctactgcg tggttgcagc gcggatgcag aacgtcgcgt aaatgaaaac cgtggcttcc
    25981 tgcaaacgct ggcgcgtctg gaaagcatca ccgtgctgcc tgccgatgac aaaggtccgg
    26041 tttccgttac taagatcatc gacggtgcag agctgctgat cccgatggct ggcctcatca
    26101 acaaagaaga tgagctggcg cgtctggcga aagaagtggc gaagattgaa ggtgaaatca
    26161 gccgtatcga gaacaagctg gcgaatgaag gctttgtcgc ccgcgcaccg gaagcggtca
    26221 tcgcgaaaga gcgtgaaaag ctggaaggct atgcagaagc gaaagcgaag ctgattgaac
    26281 agcaggctgt tatcgccgcg ctgtaatcaa aacacagtaa tgaaaacgaa ggccggagca
    26341 tgctccggcc ttttttatct cttacagaaa tggtaaagaa ggcataattc cccgtgaaat
    26401 cctggctaag aagcctttat ctggttgttc ttctttattg attaaaggaa ggtcatccaa
    26461 atcgcccagc aaatacgtat tttttgcctg ccagtcaatc atccataatt tcagcagtgg
    26521 ataagccaga ccgcccgtaa tcccggatat caccaccaac gcacacatgc gataaagcat
    26581 accgtggtac gttaaagttg agcgaaaacg aatacgccca tcattcagtg acaggttgct
    26641 cataaaatga tttcgcaacg acaccgttaa ataactggtg ctaacagcaa tcccaaaata
    26701 atagattaac tgcgcgatta tcattttacg ttgcatttcc ataaattgct gtaaattctc
    26761 aatatcatca tttgcataca cagaactatc atatgcattt aatatttgat cgaagataat
    26821 ataaccagca acagcgagaa aaggtaataa agctaaaaat gcgagaatgg catattttat
    26881 acaatacgta gtatctaatt tcaccttgaa acgatgtata ccgaaactgg tattgctcca
    26941 gagaaaactc attaccaggc tatataaagt accattaaaa ataccaatgg aaacaatacc
    27001 aactattgcc atcagaacca cagatataat aacactactt gaactattgg catgtagcat
    27061 ctttgtcgag ataaagaaaa cagtccccat cccaatggcc attaaaatcg gcaagaaaaa
    27121 ggttacccac cagaaccctt tcatagagca attaaaacta aatcttacgc cgttgagact
    27181 ggtcatcaag gcctgataac gcagtccttt tgctgccata aatataagca aaaccaacag
    27241 taacaaagtc aaaacacagc caacaagtgg catatctgct gacactgtca ttaagattgc
    27301 gaaatagcaa aaaacaaaaa caagacaact aacaaaaaca ttcccaccag taattccata
    27361 agaaaatcgt tgtccgttaa cttccatatt agcatagaga taacgcttac atttcattaa
    27421 tgcccatggc aaatagatac ctagcgttat aatcgttaac aacacattca ccacacaaat
    27481 aagaaaatat ctctcacctg taccatgaaa aacaaacgaa tgggacggaa catccatttc
    27541 attaataact tgagccataa aaattccctg aattaaaaaa tccattgata taatttatat
    27601 atacagacag caaaatgttc tgccacgtta caatactctt atcagtaata ggctggcaaa
    27661 aactttatta ctttataagt cgcttgcgca cgtaagaaga gagtggtatt aaacaaatgt
    27721 ataattactt tatttttatt gggttaaact atgaataaca ttgcgccgca atcacctgta
    27781 atgcgtcgcc tgacgcagca ggataatccc gctatcgccc gcgtcattcg tcaggtatcc
    27841 gccgaatacg gtcttaccgc tgataaaggc tacaccgtcg ccgatccgaa ccttgacgag
    27901 ttgtatcaag tgtatagtca gcctggccat gcatattggg tggttgagtt cgatggtgaa
    27961 gtggtcggcg gcggtgggat tgcgccatta accgggagtg aatcggacat ttgcgaactg
    28021 caaaagatgt attttcttcc ggctattcgc ggtaaagggc tggcgaaaaa actggcctta
    28081 atggcgatgg agcaggcacg agagatgggc ttcaaacgct gctatctgga aacgaccgct
    28141 tttttaaagg aagccattgc gctttatgag catttgggct ttgagcatat caactatgcg
    28201 cttggctgca cgggccatgt cgattgtgaa gtgcggatgc tacgcgaact ctaaaaaaat
    28261 gcccgctagt gtgtgctatc gggcatttta atcaattaat tagtggcgaa caccgtcatc
    28321 gtcttcatcg acaaaatctt catcgtcgcc gtcttcgcca ttgggatctt caaagtaagt
    28381 gccccaaccg tcgtactcaa cgtcaaattt ctccgccagc gtcatcagtt gttcaacctg
    28441 ggcatcgatc agatcggcat tcaacgcgca ctcgctgagg atgtcgcagc aaatcacgat
    28501 atcaccatct tcaacttcca gctcttctgg atcggtcact tcgtaaccga gtttaaacgc
    28561 ttcaactgct gctttttcca gggtttccag atcgtctgcg gaaagatggt gttcgatggt
    28621 gtacagcgcg tccggatcgc tgccatcttc cagtaattct tcaataatca aacgtgtttc
    28681 ttcacgctgt tcttccagtt gttccgggtt tgccatggct cattcctctt aatgtcctgc
    28741 cgatactctt attgtcacac accactgaca ttgcctccac ctttgtgaca aagatttatg
    28801 ctttagactt gcaaatgaat aaccatccat ataaattgaa ttttaattca ttaaggcgtt
    28861 agccacagga gggatctatg tccgggtttt atcataagca tttcctgaaa ttactcgatt
    28921 tcacgccagc tgaactcaac agcctgctgc agttagccgc gaagctgaaa gccgataaga
    28981 aaagcggcaa agaagaagcc aaactcactg gtaaaaacat cgcgctcatc ttcgaaaaag
    29041 actcgacccg tacccgatgc tctttcgaag ttgccgcata tgaccagggt gctcgcgtta
    29101 cttatctcgg cccaagcggc agtcagattg gtcataaaga gtcgattaaa gacactgccc
    29161 gcgtgctggg tcgcatgtat gacggtattc agtatcgcgg ctatggtcag gagattgtcg
    29221 aaacactggc ggaatacgct ggcgtgccgg tatggaacgg cctgaccaat gagtttcacc
    29281 ccacgcagtt gctggcggat ctcctcacca tgcaggagca tttacccggt aaagcattca
    29341 acgaaatgac gctggtctat gcaggtgacg cacgtaacaa catgggcaat tcgatgctcg
    29401 aagctgcggc gcttaccggt ctggatttgc gtctggtcgc gccacaggca tgctggccgg
    29461 aagctgcgct ggttacggaa tgccgcgccc tggcacagca aaatggcggg aatattacgc
    29521 tgacggaaga tgtagccaaa ggagttgaag gtgctgactt tatctatacc gatgtatggg
    29581 tgtcgatggg ggaagcaaaa gagaaatggg cagaacggat tgcattactg cgtgattatc
    29641 aggtgaacag caagatgatg cagttgaccg gtaacccgga ggtcaaattc ctccactgcc
    29701 tgcccgcgtt tcatgacgac cagacgacgc ttggtaagaa aatggcggaa gagtttggcc
    29761 tacatggcgg aatggaagtg actgatgagg tcttcgaatc tgccgccagc attgtttttg
    29821 atcaggcgga aaaccgcatg cataccatca aagcggtgat ggtcgcgacg ctcagtaaat
    29881 aattgatatc gctataaaga tgccgggaaa tacaaaaaca tcccggcatc acattatttt
    29941 atgcagtaat agttcaatta ttccatccat atttttctat tgctatacgt aaatcttccg
    30001 gaggattttc atcattcttg ttctaatttt taattcatta accatctttt cttcaaaaat
    30061 cttaacgaca taacccggat tgctttttac aatttcattt gttt
//
LOCUS       DAZGAN020000051        29659 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGAN020000051 DAZGAN020000000
VERSION     DAZGAN020000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.051"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(60..1889)
                     /gene="glmS"
                     /locus_tag="ACHKYO_RS12765"
                     /old_locus_tag="pgaptmp_002553"
     CDS             complement(60..1889)
                     /gene="glmS"
                     /locus_tag="ACHKYO_RS12765"
                     /old_locus_tag="pgaptmp_002553"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGU4429387.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2051..3421)
                     /gene="glmU"
                     /locus_tag="ACHKYO_RS12770"
                     /old_locus_tag="pgaptmp_002554"
     CDS             complement(2051..3421)
                     /gene="glmU"
                     /locus_tag="ACHKYO_RS12770"
                     /old_locus_tag="pgaptmp_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGU4429388.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLLDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3772..4191)
                     /gene="atpC"
                     /locus_tag="ACHKYO_RS12775"
                     /old_locus_tag="pgaptmp_002555"
     CDS             complement(3772..4191)
                     /gene="atpC"
                     /locus_tag="ACHKYO_RS12775"
                     /old_locus_tag="pgaptmp_002555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGU4429389.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4212..5594)
                     /gene="atpD"
                     /locus_tag="ACHKYO_RS12780"
                     /old_locus_tag="pgaptmp_002556"
     CDS             complement(4212..5594)
                     /gene="atpD"
                     /locus_tag="ACHKYO_RS12780"
                     /old_locus_tag="pgaptmp_002556"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGU4429390.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5621..6484)
                     /gene="atpG"
                     /locus_tag="ACHKYO_RS12785"
                     /old_locus_tag="pgaptmp_002557"
     CDS             complement(5621..6484)
                     /gene="atpG"
                     /locus_tag="ACHKYO_RS12785"
                     /old_locus_tag="pgaptmp_002557"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGU4429391.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6535..8076)
                     /gene="atpA"
                     /locus_tag="ACHKYO_RS12790"
                     /old_locus_tag="pgaptmp_002558"
     CDS             complement(6535..8076)
                     /gene="atpA"
                     /locus_tag="ACHKYO_RS12790"
                     /old_locus_tag="pgaptmp_002558"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGU4429392.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8089..8622)
                     /gene="atpH"
                     /locus_tag="ACHKYO_RS12795"
                     /old_locus_tag="pgaptmp_002559"
     CDS             complement(8089..8622)
                     /gene="atpH"
                     /locus_tag="ACHKYO_RS12795"
                     /old_locus_tag="pgaptmp_002559"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGU4429393.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8637..9107)
                     /gene="atpF"
                     /locus_tag="ACHKYO_RS12800"
                     /old_locus_tag="pgaptmp_002560"
     CDS             complement(8637..9107)
                     /gene="atpF"
                     /locus_tag="ACHKYO_RS12800"
                     /old_locus_tag="pgaptmp_002560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGU4429394.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9169..9408)
                     /gene="atpE"
                     /locus_tag="ACHKYO_RS12805"
                     /old_locus_tag="pgaptmp_002561"
     CDS             complement(9169..9408)
                     /gene="atpE"
                     /locus_tag="ACHKYO_RS12805"
                     /old_locus_tag="pgaptmp_002561"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGU4429395.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9455..10270)
                     /gene="atpB"
                     /locus_tag="ACHKYO_RS12810"
                     /old_locus_tag="pgaptmp_002562"
     CDS             complement(9455..10270)
                     /gene="atpB"
                     /locus_tag="ACHKYO_RS12810"
                     /old_locus_tag="pgaptmp_002562"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGU4429396.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10279..10659)
                     /gene="atpI"
                     /locus_tag="ACHKYO_RS12815"
                     /old_locus_tag="pgaptmp_002563"
     CDS             complement(10279..10659)
                     /gene="atpI"
                     /locus_tag="ACHKYO_RS12815"
                     /old_locus_tag="pgaptmp_002563"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HGU4429397.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11276..11899)
                     /gene="rsmG"
                     /locus_tag="ACHKYO_RS12820"
                     /old_locus_tag="pgaptmp_002564"
     CDS             complement(11276..11899)
                     /gene="rsmG"
                     /locus_tag="ACHKYO_RS12820"
                     /old_locus_tag="pgaptmp_002564"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGU4429398.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11963..13852)
                     /gene="mnmG"
                     /locus_tag="ACHKYO_RS12825"
                     /old_locus_tag="pgaptmp_002565"
     CDS             complement(11963..13852)
                     /gene="mnmG"
                     /locus_tag="ACHKYO_RS12825"
                     /old_locus_tag="pgaptmp_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGU4429399.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVGGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14231..14674)
                     /gene="mioC"
                     /locus_tag="ACHKYO_RS12830"
                     /old_locus_tag="pgaptmp_002566"
     CDS             complement(14231..14674)
                     /gene="mioC"
                     /locus_tag="ACHKYO_RS12830"
                     /old_locus_tag="pgaptmp_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HGU4429400.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14764..15222)
                     /gene="asnC"
                     /locus_tag="ACHKYO_RS12835"
                     /old_locus_tag="pgaptmp_002567"
     CDS             complement(14764..15222)
                     /gene="asnC"
                     /locus_tag="ACHKYO_RS12835"
                     /old_locus_tag="pgaptmp_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HGU4429401.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15374..16366
                     /gene="asnA"
                     /locus_tag="ACHKYO_RS12840"
                     /old_locus_tag="pgaptmp_002568"
     CDS             15374..16366
                     /gene="asnA"
                     /locus_tag="ACHKYO_RS12840"
                     /old_locus_tag="pgaptmp_002568"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGU4429402.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(16371..17822)
                     /gene="viaA"
                     /locus_tag="ACHKYO_RS12845"
                     /old_locus_tag="pgaptmp_002569"
     CDS             complement(16371..17822)
                     /gene="viaA"
                     /locus_tag="ACHKYO_RS12845"
                     /old_locus_tag="pgaptmp_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HGU4429403.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMEAFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17816..19312)
                     /gene="ravA"
                     /locus_tag="ACHKYO_RS12850"
                     /old_locus_tag="pgaptmp_002570"
     CDS             complement(17816..19312)
                     /gene="ravA"
                     /locus_tag="ACHKYO_RS12850"
                     /old_locus_tag="pgaptmp_002570"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HGU4429404.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19535..21403
                     /gene="kup"
                     /locus_tag="ACHKYO_RS12855"
                     /old_locus_tag="pgaptmp_002571"
     CDS             19535..21403
                     /gene="kup"
                     /locus_tag="ACHKYO_RS12855"
                     /old_locus_tag="pgaptmp_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HGU4429405.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21570..21989
                     /gene="rbsD"
                     /locus_tag="ACHKYO_RS12860"
                     /old_locus_tag="pgaptmp_002572"
     CDS             21570..21989
                     /gene="rbsD"
                     /locus_tag="ACHKYO_RS12860"
                     /old_locus_tag="pgaptmp_002572"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HGU4429406.1"
                     /translation="MKKGTVLNSDISSMISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            21997..23502
                     /gene="rbsA"
                     /locus_tag="ACHKYO_RS12865"
                     /old_locus_tag="pgaptmp_002573"
     CDS             21997..23502
                     /gene="rbsA"
                     /locus_tag="ACHKYO_RS12865"
                     /old_locus_tag="pgaptmp_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HGU4429407.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23507..24472
                     /gene="rbsC"
                     /locus_tag="ACHKYO_RS12870"
                     /old_locus_tag="pgaptmp_002574"
     CDS             23507..24472
                     /gene="rbsC"
                     /locus_tag="ACHKYO_RS12870"
                     /old_locus_tag="pgaptmp_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGU4429408.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGININK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24497..25387
                     /gene="rbsB"
                     /locus_tag="ACHKYO_RS12875"
                     /old_locus_tag="pgaptmp_002575"
     CDS             24497..25387
                     /gene="rbsB"
                     /locus_tag="ACHKYO_RS12875"
                     /old_locus_tag="pgaptmp_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HGU4429409.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25513..26442
                     /gene="rbsK"
                     /locus_tag="ACHKYO_RS12880"
                     /old_locus_tag="pgaptmp_002576"
     CDS             25513..26442
                     /gene="rbsK"
                     /locus_tag="ACHKYO_RS12880"
                     /old_locus_tag="pgaptmp_002576"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGU4429410.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26446..27438
                     /gene="rbsR"
                     /locus_tag="ACHKYO_RS12885"
                     /old_locus_tag="pgaptmp_002577"
     CDS             26446..27438
                     /gene="rbsR"
                     /locus_tag="ACHKYO_RS12885"
                     /old_locus_tag="pgaptmp_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HGU4429411.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27404..28831)
                     /gene="mdtD"
                     /locus_tag="ACHKYO_RS12890"
                     /old_locus_tag="pgaptmp_002578"
     CDS             complement(27404..28831)
                     /gene="mdtD"
                     /locus_tag="ACHKYO_RS12890"
                     /old_locus_tag="pgaptmp_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HGU4429412.1"
                     /translation="MSDKKNRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28854..29546)
                     /gene="yieP"
                     /locus_tag="ACHKYO_RS12895"
                     /old_locus_tag="pgaptmp_002579"
     CDS             complement(28854..29546)
                     /gene="yieP"
                     /locus_tag="ACHKYO_RS12895"
                     /old_locus_tag="pgaptmp_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HGU4429413.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7128 a         7608 c         7572 g         7351 t
ORIGIN      
        1 tgatgtcagt tataaaaaag ggccaccggt cggcagccct tttttatcta tgatagtgat
       61 tactcaaccg taaccgattt tgccaggtta cgcggctggt caacgtcggt gcctttgatc
      121 agcgcgacat ggtaagccag cagctgcagc ggaacggtgt agaagatcgg tgcaatcacc
      181 tcttccacat gcggcatctc gatgatgtgc atgttatcgc tacttacaaa acccgcatcc
      241 tgatcggcga agacatacaa ctgaccgcca cgcgcgcgaa cttcttcaat gttggatttc
      301 agtttttcca acagttcgtt gttcggtgca acgacgataa ccggcatatc cgcgtcaatc
      361 agcgccagcg ggccgtgttt cagctcgcct gcagcgtagg cttcagcgtg aatgtaagag
      421 atctctttca gtttcaatgc gccttccagc gcgattgggt actgatcgcc acggcccagg
      481 aacagcgcgt gatgtttgtc agagaaatct tctgccagag cttcaatgcg tttgtcctga
      541 gacagcatct gctcaatacg gctcggcagc gcctgcagac catgcacgat gtcatgttca
      601 atggaggcat ccagaccttt caggcgagac agcttcgcca ccagcatcaa cagcacagtt
      661 aactgagtgg tgaatgcttt agtggatgcc acgccgattt ccgtacccgc gttggtcatc
      721 agcgccagat cggattcgcg caccagagaa gaacccggaa cgttacagat tgccagcgaa
      781 ccaaggtaac ccagctcttt cgacagacgc aggcccgcca gggtatccgc ggtttcgcca
      841 gactgtgaca aggttatcat caggctgtta cgacgcacgg cagatttgcg atagcggaat
      901 tcagaggcga tttcgacgtc gcacggaata cctgccagcg attcaaacca gtaacgggaa
      961 accataccgg agttataaga agtaccacag gcgaggatct gaatatgctc aaccttcgac
     1021 agcagttcgt cggcgttcgg tcccagctcg cttaaatcaa cctgaccgtg gctgatgcgc
     1081 ccggtaaggg tgttttttat cgcgttcggc tgttcgtaga tctctttctg catgtagtga
     1141 cggtaaatac ctttatcgcc cgcgtcatat tgcagattgg attcgatatc ctgacgtttt
     1201 acttccgcgc cagttttatc gaagatgttt accgaacggc gagtaatttc cgcaatatcg
     1261 ccctcttcaa ggaagataaa gcgacgggtc accggcaaca gcgccagctg gtcagaagcg
     1321 ataaagtttt cgcccatacc caggccaatc accagcggac taccagaacg tgccgccagc
     1381 agggtatccg ggtgacggga gtccatgatc actgtaccgt acgcaccacg cagttgcggg
     1441 atagcacgca gaacagcctc acgcagagta ccgccctgtt tcagctccca gttcaccaga
     1501 tgggcaatta cttcggtgtc tgtttcagaa acgaaggtat agccacgcgc ttttaactct
     1561 tcgcgcagcg gttcatggtt ttcgatgatg ccgttatgca ccaccacaat gtgttcagaa
     1621 acatgcggat gcgcattcac ttctgaaggt tcaccgtggg tcgcccagcg agtatgagca
     1681 atacccgtac cgccatgcag aggatgttct tccgctgcct gagccagcat ctggacttta
     1741 ccgaggcgac gcaggcgggt catatgacct tccgcatcaa caacggccag accggcagag
     1801 tcatatccgc ggtattccag acgacgtaaa ccttcaagaa ggatttctgc tacatcacgt
     1861 tgcgcgatcg cgccaacaat tccacacata gtttttgatt ccgatttata tcgttgtcgg
     1921 tcaacctgta tgcccgtttt ttcgggcgcc ccgagccttg tagagagtgg ggttattttt
     1981 atagttactg cttgtgggag ggagattatg ttatctcctc atcccatgtg accggattac
     2041 ccggccagaa tcactttttc tttaccggac gacgccagcc ttctttctga gtctgcggca
     2101 cacggctgat agctaatgca ttttcgccga cattacgcgt cacagttgta cccgcagcaa
     2161 tggtcgcgcc tttgcctact gttaccgggg ccaccagctg agtgtcggaa ccgacaaaca
     2221 catcgtcgcc gataatggtc ttaaatttat tcgcaccatc gtagttgcag gtaatggttc
     2281 ccgcgccgat gttaacgtta tcgccaattt ccgcatcgcc caggtaagtc agatgaccag
     2341 ctttcgagcc tttacccaga cgcgcttttt tcatctcaac aaagttaccg acgtgtgcac
     2401 cttccagcaa ctcagcacca ggacgcagtc gggcaaacgg gccaatagta caggccgctg
     2461 ccagattcgc gtcttccaca acggtatacg gactgatttc gcaatcatcg ccaatcacgc
     2521 tgtttttaat cacgcaaccg gtgccgattt tcacgcgatg accgagagtc acgttgccct
     2581 cgatgataac gttagtatca atttcaacat cgcgcccgtg agtaagcgta ccgcgcaaat
     2641 caaaacgcgc cggatcgagc agcataacgc ctgctaacag cagtttttca gcctgttcgg
     2701 actggtaaac gcgctccaga cgggagagtt gcaggcggtt attcacacct tctacttcgc
     2761 ttaaacgttg cggatgaacg gcgacgattt cacgcccttc ctgatacgcc agcgcaataa
     2821 tgtcggtgat gtagtattcg ccctgagcat tattgttggt cagcttcgcc agccagcgtt
     2881 tcatatctgc gccgttagcg atcagaatgc cggtgttgat ctcctgaatc tgacgctgct
     2941 cgtcggtggc atctttgtgc tcaacaatgc cggtaacttt gccattttca cgggtgatac
     3001 gtccataacc ggtcggatca tccagtttca ccgtcagcag accaatgcca ccctgcggtt
     3061 tagcatcacg caggcgctgg agtgtttcga cagagatcag cggcacgtcg ccgtagagca
     3121 ttaaaatgtc ttcatcatcg gcaaagaaag gtgcggcctg ctgcattgca tgacccgtac
     3181 ccagctgctc tgcctgaagc acccagttaa ggttgtcgtc tttcagcgcc tgtttaagca
     3241 gatcgccgcc gtgaccgtac accaggtgaa cgtgcgctgc gcctaattca ttcgcagcat
     3301 caatgacatg ctgaaccatc gctttcccgg caagggtatg cagcactttc ggaagatcgg
     3361 aatacatgcg cgtgcctttg cctgcggcaa ggatcactac gctcatagca ttattcaaca
     3421 tacgcgtcct gactgtaatt tgagaacgaa tttaaaccgc ttcaccttga aaaaactaca
     3481 tttttttcat cgtgaaatgg acagaggata aattgttcaa taacggatta tccccgctga
     3541 cgaaagtgcc attttcgacc atcgtcacgc cttttgtctg cagaaaatta aggcagtacc
     3601 ttcatttaag cgtcaggtgg atgtttttgc tcttatttcg atcaatgaat aaacgggaaa
     3661 acaggggtgt tttatatcag caggatctat gtgaacgctc ttcaggacgg atcacgcgta
     3721 aaaaaaagcc agcctgtttc cagactggct tttgtgcttt tcaagccggt gttacatcgc
     3781 ttttttggtc aactcgataa cgcgcagctg cgcgatcgct ttggccagtt ccgcagacgc
     3841 ctgagcgtaa tctacgtcgc cgtgagagct gctaatgtgc tcttcagcct tacgtttcgc
     3901 ttccatggct cgcgcttcgt cgagatcctg gccgcgaatt gcggtgtcgg ccagaacggt
     3961 cacgttgcca ggctgcactt caagaatgcc gccagacaga tagataaact cttcgtgacc
     4021 gtgctgtttc acgatgcgaa tcataccagg cttaatggcg gtgagcagcg gtgcgtggcc
     4081 agggtagatc cccagttcac cttcgctacc cgttacctgg attttctcga ccagaccaga
     4141 gaacatttgt tgctctgcgc tgacgacgtc caggtggtaa gtcattgcca tatcaccctc
     4201 cgattaaggc gttaaagttt tttggctttt tccacagctt cttcgatgga accgaccatg
     4261 tagaacgcct gctccggcag gtgatcgtat tcgccttcca tgatgccttt aaagccacgg
     4321 atggtgtctt tcagggagac gtatttaccc ggagaaccgg tgaatacttc tgccacgaag
     4381 aacggctggg acaggaagcg ctggatctta cgagcacgcg ctaccaccag tttgtcttct
     4441 tcagacagtt catccatacc caggatggcg atgatgtctt tcagttcctg ataacgttgc
     4501 aggatggact gaacgccacg cgcggtgtcg tagtgttcct gaccaaccac cagcgggtcc
     4561 agctgacggc tggtggagtc cagcgggtca acggccgggt agatacccag agacgcgatc
     4621 tgacggctca gtaccacggt tgcgtcaagg tgcgcaaagg tggttgccgg agacgggtca
     4681 gtcaagtcat ccgcaggtac gtatactgcc tgtacggagg tgatagaacc agttttggtg
     4741 gaggtgatac gttcctgcag aacgcccatc tcttccgcca gggtcggctg ataacctacc
     4801 gctgaaggca tacggcccag cagtgcggat acttccgtac ctgccagggt gtaacgatag
     4861 atgttgtcaa cgaacagcag aacgtcacga ccttcgtcac ggaatttctc agccatggtc
     4921 agaccggtca gagcaacgcg cagacggttt cccggcggct cgttcatctg gccatacacc
     4981 agggatactt tgtcgataac gttggagtcg gtcatttcgt ggtagaagtc gttaccctca
     5041 cgagtacgtt cacctacgcc cgcaaacaca gagtaaccgg agtgctcgat cgcgatgtta
     5101 cgaatgagct ccatcatgtt tacggtttta cctacacccg caccaccgaa cagaccaact
     5161 ttaccgccct tagcgaacgg acacatcagg tcgataactt tgataccggt ttccagcagt
     5221 tcctgagagt ttgacagctc ttcgtaggaa ggtgctgcgc ggtgaatcgc ccaacgctct
     5281 tcttcaccga tctcgccttt catgtcgacc ggttcaccca gtacgttcat gatacggccc
     5341 agagtcgctt tacctaccgg gacttcgatc gggtgttcga ggtcttttac atccagaccg
     5401 cgacgcagac cgtcggagga acccattgcg atggtacgca cgataccgcc gccgagctgc
     5461 tgctgaactt ccagcaccag acgctcatta ccattttgca cctcaagagc atcgtacacg
     5521 cgcggtacgg catcctgagg gaattcgacg tcaactacgg cgccgattac ctggacaatc
     5581 tttccagtag ccatcttaaa tcctctacga aataacctgt ttaaaccgcg gcggcccccg
     5641 agacgatctc ggtgagttcc tgagtaatgc tggcctgacg agctttgttg tataccaact
     5701 gcagctcttt aatcaggctg ccgccattgt cggtcgcggc tttcatcgcc accatacggg
     5761 cggcctgctc gctggccagg ttttcaacca cgccctgata aacctgagat tcgacataac
     5821 gacgcagcag ggtatccagc aacgccttcg gatcgggttc gtacaggtaa tcccaggatt
     5881 tatgtttcag atcatcatca tctgatgccg gtaacggcag cagctggctg atggtcggaa
     5941 cctgagacat ggtgttaata aatttgttgc tgacaatgta aagcttgtcc agacggcctt
     6001 cgtcgtaggc ctgcaacatc acttttaccg gaccgatcag ttcggacagg gaagggttat
     6061 cccccatgcc ggtgacctgg gcaacaacat tgccgcccac ggagttgaag aacgacacgc
     6121 ctttcgagcc gatcattgcg aggtcgcatt gaacgccttt gtcggtccag gtcttcattt
     6181 ccgccagcag ttttttgaac aggttaatgt tcaaaccacc gcacaaacca cggtcggtcg
     6241 acaccaccag gtagcccacg cgtttaacgt cgcggtcttc caggtaaggg tgcttatatt
     6301 ccagattacc gtgtgcaagg tgaccaatca ctttgcgcat ggtttctgca taaggacggc
     6361 tggccgccat gcgatcctgc gatttacgca ttttggaagc ggcgaccatc tccatcgctt
     6421 tagtgatctt ttgcgtgttc tggacgcttg cgatcttact acgtatctct tttgcgccgg
     6481 ccatgagctt ctcctcaatg ccttgcggcc tgccctaagg caagccgcca gacgttacca
     6541 ggattgggtt gctttgaagg aatcgaggat gcctttcagc ttgccttcga tttcgtcgtt
     6601 gtagccaccg gtctggttga tctcttgcat caacggagcg tgatcacggt cgacgtaagc
     6661 cagcagagcg gcttcgaagc tgccaatttt cgacagttca acatccgcca ggtaaccacg
     6721 ttctgctgcg aacagaacca gagactgctg cgcaacggac atcggcgcat actgtttctg
     6781 tttcagcagt tcggtcactt tctgaccgtg gtcaagctgc ttacgtgttg catcgtcaag
     6841 gtcggatgca aactgagaga acgctgccag ttcacgatac tgtgccagag cggtacggat
     6901 accaccggac agttttttca tgatcttggt ctgtgctgca ccaccaacac gggatacgga
     6961 aatacccggg ttaaccgcag gacgaatacc ggcgttgaac aggttggttt ccaggaagat
     7021 ctgaccatcg gtaatggaga ttacgttggt cggaacgaac gcagaaacgt cacccgcctg
     7081 agtttcgata atcggcagtg cggtcagaga accggttttc cctttcactt cacctttggt
     7141 gaaggcttca acgtattcgg cgttaacacg tgcagcacgc tccagcagac gagagtggag
     7201 gtagaaaacg tcgcccggga atgcttcacg tcctggcgga cgacggagca gcagggagat
     7261 ctgacggtaa gcaacagcct gtttagacag gtcatcgtaa atgatcagcg catcttcacc
     7321 gcggtcacgg aagtattcgc ccattgcgca accggcatac ggtgccaggt attgcagtgc
     7381 agcggattca gacgcggttg ctaccacaac gatggtgtta gccagtgcgc cgtgctcttc
     7441 cagtttacgt accacgttag aaatggtgga cgctttctgg ccgatagcga catagataca
     7501 tttgataccg gaatcgcgct ggttgatgat ggcatcgata gccagtgcgg ttttacctgt
     7561 ctgacggtca ccgatgatca attcacgctg accacgaccg attgggatca tggagtcaac
     7621 ggctttataa ccggtctgta ccggctgatc tacggactga cgttcgataa cgcccggagc
     7681 gattgcttct acagcagaga agccgtcgtg atccagcgga cctttaccgt cgattggtgc
     7741 acccagagtg ttaaccacac ggcccagcag gccacggcca accggaactt ccaggatacg
     7801 gccagtacac ttaactttca tgccttcggc aaggtcagcg tacggaccca taacaaccgc
     7861 acctacagag tcgcgctcga ggttcagtgc gatagcgtaa cggttacccg gcagggagat
     7921 catttcaccc tgcatacaat cggccaggcc gtgaatgcgg ataacaccgt cacttacaga
     7981 aacaatagta ccttcgttgt gagcttcact cacaacattg aactgagcaa tgcgctgctt
     8041 gatcagttcg ctgatttcgg tggaattcag ttgcatgctc cagtcccctt aagactgcaa
     8101 gacgtctgca aggcgctcaa gacgaccgcg tacgctgcca tcaatgacca tatcacccgc
     8161 tcggatgata acgcctgcca ttacagactt atcgattttg caattcagct taactttgcg
     8221 tgacagacgt ttttccatcg cagcagaaat tttcgcgagc tgttgttcac tcagtgcggc
     8281 agcggaaatg acgtctacct cagcggtagc ctcactcacg gcacgaaggt gaataaactg
     8341 ctccagaaca tccgggagcg cgttaagacg accattttca gccattaccc gaatcaggtt
     8401 ctgaccgttt tcgtccagtt gctcaccaca aactgcgata aacgactcgg cgagcgtttc
     8461 tggcgcaagc gcgccagaga gaagctctgc catttgttcg tttttggtta cctcggcggc
     8521 aaacgccagc atgtcctgcc agcgttctac actttggtgt tcgacggcaa agtcaaaagc
     8581 tgctttggcg taggggcgag ctaccgtaat aaattcagac atcagcccct ccctccttac
     8641 agttcagcga caagtttatc cacgatgtcg ctgttagcag cttcatccac ggaacgttcg
     8701 atgatcttct cggcgccagc aacagccagg atagcaactt gcttacgcag ctcttcacgg
     8761 gcacgtttac gctcggcttc aatttctgcc tgcgcctggg ccacgatttt agtacgttcc
     8821 tgttctgcct cagctttcgc ttcgtccaga atctgcgagc ggcgtttatt cgcctgctcg
     8881 atgattacct gggcttccgc tttcgctttt ttcagctggt cggtcgcgct ggcctttgca
     8941 aggtcaaggt ccttatgtgc tcgttctgcg gaagcaaggc cgtcagcaat ttctttttga
     9001 cgtttttcga tggctgccat taatggcggc catacgtact tcatgcagaa cagaacgaac
     9061 aggacaaacg cgatggcctg gccgaggatt gttgcgttaa gattcacagc acaatgcctc
     9121 tatttagtta acgttctgat attgctcttt aaataaaagc aacgcttact acgcgacagc
     9181 gaacatcacg tacagaccca gacctacagc gatcatcggg atagcatcca ccagacccat
     9241 aacgataaag aactgagtac gcagcagagg aatcagatca ggttgacgcg ctgcgccttc
     9301 caggaattta cccccgagga tgccgatacc gatcgcagca ccgattgccg ccagacccat
     9361 catcacagcg gcagccatgt acagcagatc catattcagg ttttccatga cagtctccag
     9421 tttgtttcag ttaaaacgta gtagtgttgg taaattaatg ttcttcagac gccatcgaca
     9481 gatagacgat cgtcagaacc atgaagatga aggcttgcag cgtaatgatc aggatgtgga
     9541 aaatggccca cggcacattc aggatccact gtgaccacca cggcaacaga ccagcaatca
     9601 gaatgaaaat cagctcaccg gcatacatgt taccgaacag tcgcaaacca agtgaaactg
     9661 gtttggacag caggcttacc ccttcaagga ttaagttgac aggaatgaac gcccagtgat
     9721 tgaacggctg cagcgtcaac tctttcgtga agccgccgat gcctttcatt ttgatgctgt
     9781 agaacagaat caggataaat acgcccaatg ccatagacag cgttacgttc acgtccgcag
     9841 acggaaccac acgcagtgca ggcagaccca gtacatgttc agcaatgtac ggcagcaggt
     9901 cgataggcag taaatccatc aggttcatca ggaataccca gacgaagatc gtcagggcca
     9961 gcggagcaat cagcttgctt ttgccatggt acatgtcttt cacgctacca ttaacaaagc
    10021 cgatcaccag ctcaatcgcg gtctgaaact tacctggcac accgctggtc gcctttttgg
    10081 ctacgctacg gaataaaacc aggaacaaca gacccagcac caccgagaag aacatggagt
    10141 caatattgat tgtccagaag gtggctgggg ggttttgtgg atccaccagc gagaatgtac
    10201 gcaggtccag ctgaaggtta ttcaggtggt gtcctatgta atcctgcggc gtcatatttt
    10261 ctgaagccat gatgcctttt accctttgtt gttaattaca gccggtgcca gtatctgaac
    10321 caccagcacc aaaacccacg taacgatcag cggcaagaat accgccttta aaaccgccaa
    10381 cgccaccacc agtaacacca acatcgccag aactttgaaa gcttcgccaa atgcgaatgt
    10441 ccaggccacc cggcctttcg ctggtgtatg cgcctggtga cgccaggcaa atatcataaa
    10501 caaaacgtta ggcagaaaga ctgccaggcc cccgcttatt gcagagacgc cccagaaggg
    10561 gtctttgagg ctgaacagca atccacttgc tatcaccacc agtaactgaa cgagcagaag
    10621 cttccgagca acgtttcgac tcacgagcga cacagacatc acgtttttca ctcctgctcc
    10681 cttcgaggta tgccgcgtgt cgtataaaac tttctttaag gcttagagtc aagcatcaaa
    10741 aagcggtcaa attatacggt gcgcccccgt gatttcaaac aataagtagc caaaaggtga
    10801 ataaatgttt aaatattttt ccagtgcata caattgcgac ttttctgcta accctgttcg
    10861 atcatgaaaa actgtaaata acgcgtaaac actggtgata aagcgtgctt cagatcacat
    10921 attgcgcatg ttcgcgcaca gcatatttat ttacttggca aatgatgcct ttgcaagttt
    10981 atgatatttc agtctaaaaa cagatactgt tttaataaat gacatttaca caacaaaaac
    11041 cacccattga catttttaat aatgttttaa cagccaatga tggttcttag cgccgatttt
    11101 tagcagactg atattttcac taatgactta ttttctgctt accaaaaaaa gccacgttat
    11161 cttgttgatg caaaagagtg aacgtggcgt taaatgtaac cagttatatc agtagaaaac
    11221 ctggttgttg ttaacagtct aaccggtcaa ttttttatga tttttttgat aaaaattaaa
    11281 ttttatttgc tttaatcacc accagatgac gttcgccatc cagggctgga acctgaagtt
    11341 taaccactga ttcgacctga tattcttcgg gcaacaaagc gatttcatct tccggcattt
    11401 gccctttcag cgcgtagaaa cggccttgct caccaggaag atggtggcac cagctcacca
    11461 tatcgttcag agaggcaaaa gcgcggctaa ttacgccatc aaatggcggc tctgaaggaa
    11521 actcttctac cctgctctgt actggttcaa tattctccag tttaagctca tgttgcacct
    11581 gacgaaggaa acgcacgcgt ttaccaaggc tatccaacag agtgaaatgg gcttcaggac
    11641 gcacgataga gagtggaatg cctggcagtc ctggtccggt gccgacatcg ataaaccgtt
    11701 caccttgcag atacggtgcc accacaatgc tatcgagaat atggcgtacc agcatctcat
    11761 taggatcgcg gaccgaagtc aggttgtacg ctttgttcca tttatgcagc atattcacgt
    11821 aggcaataag ctggtttttc tggtgatcgg taagcgaaat acctgcgtct ttcagcagta
    11881 aggagagttt gttgagcacg gtgattacct gttcttgatg cgttgcctgg taagcgggtg
    11941 cttaccaggc atttttaatg cgttatgcgc tacgacgcag cataccctgt tttttcagcc
    12001 acaccagcag aatggagatg gccgcaggcg tgacgccaga aatacgcgaa gcttggccga
    12061 tagaggctgg tttgtgatcg ttaagtttgg cgatcacttc gttagaaaga ccggatacct
    12121 ggcggtaatc cagtgtcgcg ggtagcaggg tgttctcgtt acgcagctgc ttttcgatct
    12181 catcttgctg gcgcgcgata taaccttcgt atttaacctg aatctcaacc tgttccgccg
    12241 cctgttcgtc tgtcaacgca ggggcaaacg gcgtcagcgt ggttaatttt tcataagtca
    12301 tttccggacg acgcagcaga tcttcaccac tggcttcacg ggaaagcggc gcagtcaggt
    12361 gagcattcac ttcggctgca gcttccgccg acggggttac ccaggtcgat ttcagacgct
    12421 gacgctcacg ctcgatattc tcaagtttct cgttaaagcg cgcccaacgt tcgtcatcca
    12481 ccaggcccag ttcacgaccg atttcagtca aacgcagatc cgcattatct tcgcgtagca
    12541 tcagacgata ttctgcgcgc gaagtaaaca tacgatacgg ttctttggtt cctaaagtgc
    12601 acaggtcatc aactagtacg ccgagatacg cctgagaacg tgccggagcc caaccttctt
    12661 tgtcagcaga cagacgggca gcgttaagac cggccagcaa accttgcgca gcggcttctt
    12721 cgtaaccggt agtgccgtta atctgaccag caaagaacag cccctggata aacttgctct
    12781 ccagcgtcgg tttcaggtcg cgaggatcga agaagtcata ctcaatggca taacccggac
    12841 gcacgatctt cgcgttttcc atcccctgca tagagcggac gatttgcatc tgcacatcga
    12901 acggcaggct ggtggagata ccgttcggat aaatttcatt agaggtcagt ccttccggtt
    12961 caaggaagat ctgatgctga tttctgtcgg cgaagcgcat aactttgtct tcgatcgacg
    13021 ggcagtagcg tgggccgaca ccttcgatca cccctgcata cattgggcta cgatcgaggt
    13081 tactgcggat cacatcatgg gttttctcgt tggtatgagt gatataacac ggcacctgct
    13141 ggggatgctg ggacgcattg cccataaacg agaataccgg cattgggtta tcgccatgct
    13201 gttgcgccag cacactaaag tcgatagttc gagcatcaat acgcggtggt gtcccggttt
    13261 tcagacgacc aacgcgcagc ggcagttcac gcaaacggcg agaaagcgga atggacggcg
    13321 gatcaccagc acggccaccg ctgtaattat ccagaccgat atgaatttta ccgtcgagga
    13381 acgtcccaac ggtgagcacg acggctttgg cacggaactt cagtcccatt tgggtaacag
    13441 caccgcccac gcgatcgttt tcgacaataa gatcttcaac cgcctgctgg aagatcatca
    13501 ggttcggttg gttctccagc gccgtacgta ccgcctgacg gtagagcaca cgatccgcct
    13561 gagctcgggt agcgcgaacc gccggtcctt tgcttgcgtt tagtatccta aactggatac
    13621 ccgcttgatc gatcgctttc gccatcagac cgccgagtgc atccacttct tttaccagat
    13681 gtcccttccc aataccgccg atcgccgggt tgcagctcat ctgccccaga gtgtcgatat
    13741 tgtgtgtcaa aagcagagtc tgttgaccca tacgcgccgc ggccatcgcg gcctcggtgc
    13801 ctgcatgacc cccgccaatg atgatgacgt caaaaggatc cggataaaac atggtgattg
    13861 cctcgcataa cgcggtatga aaatggattg aagcccgggc cgtggattct actcaacttt
    13921 gtcggcttga gaaagacctg ggatcctggg tattaaaaag aagatctatt tatttagaga
    13981 tctgttctat tgtgatctct tattaggatc gcactgccct gtggataaca aggatccggc
    14041 ttttaagatc aacaacctgg aaaggatcat taactgtgaa tgatcggtga tcctggaccg
    14101 tataagctgg gatcagaatg aggggttata cacaactcaa aaactgaaca accgttgttc
    14161 cttggataac taccggttga tccaagcttc ctgacagagt tatccacagt agatcgcacg
    14221 atctgtatac ttatttgagt aaattaatcc acgatcccag ccattcttct gccggatctt
    14281 ccggaatgtc gtgatcaaga atgttgatct tcagtgtttc gcctgtctgt tttgcaccgg
    14341 aatttttgag ttctgcctcg agtttatcga tagccccaca aaaggtgtca tattcacgac
    14401 tgccaatacc gattgcgcca aagcggaccg cagaaagatc gggcttctgt tcctgcaatg
    14461 cttcatagaa aggagaaagg ttgtccggaa tatctccggc accgtgggtg gagctgataa
    14521 tcagccagat ccccgaggca gataaatctt ccaacagcgg accgtgcagc gtttcggtgg
    14581 taaaacccgc ctcttccagc ttttcagcca ggtgttctgc tacatattcg gcaccgccga
    14641 gggtgctgcc gctgataaga gtgatatctg ccataaaccg ccacctttat taagagtggc
    14701 gtattgtacg ctgtgaacgc gttgggatct acctgtggaa aagtatggga ttaaaaaagc
    14761 cgattatggc ttgatggtac gcatgatcgg gttttgcagg acgatcaatg tctcggtgga
    14821 ctgaatttca tcaattgttt ggatcttgtt gataagtaca tgctggagag cgtcgatcga
    14881 acggcacatc acttttataa agatgctgta gtggccggtt gtgtaatagg cttcagtgac
    14941 ttcatcaagg ctttccagct ttgccagcgc ggaagggtag tctttggcgc tctttaatat
    15001 aatgccgata aagcagccta cgtcataacc gagctgcttc gggctgacat caatacgcgc
    15061 cccggtaatg atccccgcct gcttcatttt ctctactcga acgtgaatcg tccccggact
    15121 gacgccaaat tgtttcgcca gttcggcgta agcggtgcgc gcattgccca ttaatgcttc
    15181 caggatgcca cggtccagat tgtcgatcag ataattttcc ataggatttt cttatgcaga
    15241 ttgatgattc attctatttt agccttcttt tttaatgaat caaaagtgtg ttaggctttt
    15301 tattgaatga ttattgcatg tgtgtcggtt tttgttgctt aatcataagc aacaggacgc
    15361 aggagtataa aaaatgaaaa ccgcttacat tgccaaacaa cgccaaatta gcttcgtgaa
    15421 atctcacttt tctcgccaac tggaagaacg tctggggctg atcgaagtcc aggcgccgat
    15481 tcttagccgt gtgggggatg gcacgcagga taacttgtcg ggctgtgaaa aagcggtgca
    15541 ggtaaaagtg aaagctctgc ctgatgccca gttcgaagtg gttcattcac tggcgaagtg
    15601 gaaacgtcag accttagggc aacacgactt cagcgcgggc gaagggctgt acacgcacat
    15661 gaaagccctt cgccccgatg aagagcgtct ttctccgttg cactcggtct atgttgacca
    15721 gtgggactgg gaacgcgtaa tgggcgacgg tgagcgtcaa ttctcgactc tgaaaagcac
    15781 ggtagaggcg atctgggcgg gaattaaagc aaccgaagct gcagttagcg aagagtttgg
    15841 tttggcaccg ttcctgccgg atcagatcca cttcgtacac agccaggagt tactgtctcg
    15901 ttatccggat ctcgatgcca aagggcgtga gcgggcgatt gcgaaagatc ttggcgcggt
    15961 attcctcgtc gggattggcg gcaagctgag tgatggtcat cgtcacgacg tgcgcgcacc
    16021 ggattatgat gactggagca ccccgtcaga gctgggccat gcgggtctga acggcgatat
    16081 tctggtatgg aaccctgtac tggaagatgc gtttgagctt tcttccatgg ggatccgcgt
    16141 ggatgccgac acgttgaagc atcagttggc gctgaccggt gatgaagatc gtctgcagct
    16201 ggaatggcat caggcgctgc tgcgcggtga aatgccgcag accatcggcg gcggtatcgg
    16261 ccagtctcgt ttgaccatgc tgctgctgca actgccgcat atcggccagg ttcagtgtgg
    16321 tgtatggcca gctgctgttc gcgagaacgt cccttctctg ctgtaataat ttatcgccgc
    16381 cagcgtctga gcaggcggct tcgcatcccg gtatcaaagc gccagatatg atcgaaaatg
    16441 cgcatgatgc cgggtttgcc gtgtgccgac atcgccacgg catgaaagcg atgctgatgt
    16501 acccgctgca gctctttcac tttactcgtc acgtcgtcag gcaaccgctg agcgataaaa
    16561 tcagaaatca ccaccgcgtc ggcatcaaac cattccctgc tttgcaagcg ttccataatg
    16621 gcgcgaaaac aactggcaag atcggtgccg ccacgaaact gctggcttaa aaaacggatt
    16681 gcttgttcga tgccttgtgg gcctgaaagc tcataacgga cgatctcggt ggaaaatagc
    16741 ataatatagc agcgccggtt ttcagcgaga gcaatgcgca tcaaggccag gcagaacgct
    16801 ttcgcacact gttcattaaa gccgcccatt gagccggaag tatccacaca gacaataaac
    16861 ggcccgcgcg gctgttcatc gtaatcttta tgtaccaccg gacgttcgat cactttttca
    16921 cgccacgact caccgtgcag gcgataggtg agcaactgtt tttccaccag ccgacggtaa
    16981 aactcatact ccagttccgt tatccctagt gtcgccagtt ctggcggcag gagacgtaaa
    17041 atatcatcgc tttgttgcag accatcaacc tgctcaggaa cagttgccgg ttcgcgcacc
    17101 atggtgcgga aggcttccat ctgcgcatcg ttacgcggta ttgatttggc ttcccgggaa
    17161 cgccccagct gttctgccag gcgtttcagt tccggctgtt cgttaagaaa ttcaccgtat
    17221 ttcacaatca actgatagtc gccacgttta agctgaccgg cgctcatatc ccacagacga
    17281 ccagctgcgg tattgttatc tgcgagaatc ggttcaagtt gtccgctcag cgtcatgcgt
    17341 tcctgaactt cacttaacag ttgttcgcgt tcttcttcta atagctgttg atttaacgtc
    17401 gttgcttgca cgatcagact taaacgccaa cgctggagga aaagcgtatg taacgctgaa
    17461 gtgatcgtgc tgttagcatc aaccaactgt cgggcttgtt ctgcccatgg ggaattcaga
    17521 cgatgcagta agtccaggat ctgtggtagc tgcacaataa actgcggcgt ggagaggagc
    17581 tggctttgct gatagcacat cacctcttcg gtgagttccg gcgggactcg ggcatctttc
    17641 agccgactgc gcagcgcctc acgccagcgg ggaacatcat cagtgattgc cgccttcagt
    17701 cgtgggaatt tttcaaagaa gactgccagc tgcggtgagg ccagcagcgc gatgatcatc
    17761 tcttcgatca atccctcttc gctgacggcc agcatcacat taagcgtatc cagcgttagc
    17821 attgttgtgc ctggcgaatc tgtgcgccga catcctgtag gctggcttca atgcgaccta
    17881 accagtcgcc agggataaac agacattttt gctgttcgct gaataaagcg tgttgcttgc
    17941 gccagtcact ttcaagctct tccagttgtt gttttatttc acctggcaga ccttcagccg
    18001 atgaaccggg aagtgccagc gtactgcctt gtaaacttac atcgcgtaca acaagatgtt
    18061 gggcgctatc cacttccaga ttcaatttct gggcaaagcc gataccgttc agtttgccgc
    18121 gaatttcacc acctttactc agccactgtt ccagcgcgtt acgctcaaag gagatatgaa
    18181 ccacttccat atcatgcaat tttaacggtt tttgcagcag cagagtcaga gtggaagcag
    18241 taacgttaac aggaagttga tactgctgac gacggctgaa aatgccgccc agacgaatta
    18301 ccgttaaggc tgttttatcg ctttgttgct gctggagttg caggtgacgt tgcacaatcg
    18361 cgccaaggcg agtcaacatc ccttgctgtt gccaggcgtg accggtcatc aatacatcaa
    18421 tttgttgttg tatcaaattc aggctttgcg cgtcatacca caggcaatct ttcagcaaaa
    18481 tgagatctac cggggcaaca gcactgcgac cgctaaaaaa ggcgctggcc tgcaataatc
    18541 ggatcgcttt tttccagcga cgatccgaga cataaggcgc atccggtaat ttatccagtt
    18601 gctggcgcag cataaaaatc agctcaaata catgatcggg cagcgtaatt tcaccaatct
    18661 ctttctgcca gcgttcatat tcttcatctg tgacctgcaa ggcatcagga accggattgt
    18721 cgttttcatc ctgttgactg gtcagcatgg agcggaaatt cgctttatcc tgcactttat
    18781 ctaaccacag acgaatcagc atgcggtcat ataacgcttc cagactgctg tctgcttccg
    18841 gcagctcgtt ggaggccgcc accagcagac gcatcgggat tttttctacg tgtgcaccgt
    18901 tgcggaactg gcgctcgtta atggcggtga gcaaggtatt aagaattgcc gggcccgctt
    18961 tccagatctc atccagaaag acaatttctg cttccggcag gtaaccgctg gttaaacgtt
    19021 catagcgccc ttcatctttt agcgcctgaa tagaaagggg accaaaaact tcttccggcg
    19081 tggagaagcg ggtcatcaga tattcaaacg cgcgggcatt ctgaaaggcg aattttaagc
    19141 gccgggcgat caaacttttg gcaatacctg gcgggccaag gaggaacaca ctttcaccac
    19201 ttaatgccgc taataaacac aagcggatgg cgtggctacg ttcataaagc cccttttcca
    19261 acgaactgct caggcgggaa attctttccg ctaataaatg agggtgagcc ataatgaagt
    19321 ggcgtccttt cgtcaaaagt tctgcgtaaa ttgcgagtat agacgtttct tgctggtggc
    19381 taaaatagtc tcaaaggggg ggtatttttc tttgagccag gttaatgtgg ccgcatttag
    19441 gagtacgatt ttgccgttaa tcgtgcatac tgtgcgcttt tttgtgggcc aagggactaa
    19501 gcacacattt catatttcaa cgaaagacta gtctatgagc actgataata agcaatcatt
    19561 gcccgcgatt accctcgcgg cgattggagt tgtctacggc gatattggta ccagcccgtt
    19621 atatacactt cgtgaatgtt tgtccggcca gtttggtttt ggcgttgaac gcgatgccgt
    19681 gtttggcttt ttatcgctga tcttctggct gctaatcttt gtggtttcca ttaaatatct
    19741 caccttcgtg atgcgggcag ataacgccgg tgaggggggg atcctgacgt tgatgtcgct
    19801 tgccgggcgt aatacgtcgg cgcgaaccac atcaatgctg gtgattatgg ggctaatcgg
    19861 cggcagcttt ttctatggtg aagtcgtcat aacacccgct atttcggtga tgtcagccat
    19921 tgaaggtctg gaaatcgtcg ccccgcagct ggatacctgg atagttcccc tctcaattat
    19981 cgttctcact ttattattta tgattcaaaa acatggcacg gctatggtcg gtaagctgtt
    20041 tgcgccgatc atgctgacct ggtttttgat tctggcagga ctggggttac gtagcattat
    20101 tgctaacccg gaagtgctgc atgcgctgaa tccaatgtgg gcggtgcatt tcttccttga
    20161 atacaaaacg gtttctttta ttgcattagg ggcagtggtg ttgtcgatta cgggggtcga
    20221 ggcgctgtat gctgatatgg ggcactttgg taagttccct attcgcctgg cgtggttcac
    20281 cgtcgtatta ccttccttga cccttaatta cttcggtcag ggagcgctgt tgttaaagaa
    20341 cccggaagcg attaagaacc cgttcttcct gttggcaccg gactgggcgc tgatcccgct
    20401 gctgatcatc gccgcactgg cgacggtaat cgcctcgcag gctgtcatct ctggcgtctt
    20461 ctcattgacg cgtcaggcgg tacgtctggg atatttgtcg ccgatgcgca ttattcacac
    20521 ctccgaaatg gagtcagggc aaatctatat tccgtttgtg aactggatgc tctatgtcgc
    20581 ggtcgtgatt gtgattgtca gctttgagca ctccagcaac ctggcggcgg cgtacgggat
    20641 tgcggtgacc ggaaccatgg tgctgacgtc tattctctcg actactgtgg cacgtcagaa
    20701 ctggcactgg aataagtatt ttgttgcgct gatcctgatt gctttccttt gtgtcgatat
    20761 tccattgttc accgctaacc tcgataaact gctctccggc ggctggttgc cattgagcct
    20821 cggtactgtg atgtttatcg tgatgaccac ctggaagagc gagcgtttcc gcttgctgcg
    20881 gcggatgcat gaacatggta actctctgga agcgatgatt gcttcgctgg agaaatcacc
    20941 gcctgttcgc gtgcccggga ccgcggtgta tatgtcgcgt gcaatcaacg tcattccctt
    21001 tgcgctgatg cataacctta aacataacaa ggtattgcat gagcgggtga ttctgttaac
    21061 tctgcgcacc gaagacgcgc catatgtcca taacgtccgt cgggtacaga tagaacaact
    21121 gtcgcccact ttctggcgcg tggtggcaag ttatggttgg cgagaaacgc caaacgtgga
    21181 agaagttttc caccgctgcg gtctggaagg attaagttgc cggatgatgg aaacctcctt
    21241 ctttatgtcg catgagtcgt tgatcctcgg caaacgcccg tggtatttgc gtctgcgcgg
    21301 caagctgtac ttgctgctgc aacgtaatgc gctgcgtgca ccagatcaat ttgaaattcc
    21361 gccaaacagg gttatcgaac tgggtactca ggtcgaaatc taacgccaga cgcctccttt
    21421 ctccataagg gggcgttttt gttttcatgg ttaatcacca tgtaaaacgt ttcgaggttg
    21481 atcacatttc cgtaacgtca cgatggtttt cccaactcag tcaggattaa actgcgggtc
    21541 agcgaaacgt ttcgctgatg gagaaaaaaa tgaaaaaagg caccgttctt aattctgata
    21601 tttcatcgat gatctcccgt ctgggacata ccgatacgct ggtggtgtgt gatgctggtt
    21661 tacccatccc caaaagtaca acgcgtatcg atatggcatt aacccagggt gtaccttctt
    21721 ttatgcaggt gctgggcgtc gtcacaaatg aaatgcaggt cgaggctgcc attatcgcgg
    21781 aagagatcaa acaacataat ccgcaactcc acgaaacgtt gctcactcac cttgagcagc
    21841 tgcaaaaaca ccagggaaat accattgaaa ttcgttacac cacgcatgaa caattcaaac
    21901 aacaaaccgc agaaagtcag gcggtaattc gcagcggaga atgttctccg tatgcgaata
    21961 tcattctctg tgctggcgtg acgttctgag gccgtcatgg aagcattact tcagcttaaa
    22021 ggcatcgata aagccttccc gggcgtaaaa gccctctcgg gcgcagcgtt aaatgtctat
    22081 ccgggccgcg tgatggcgct ggtgggcgaa aacggcgcgg gtaaatccac catgatgaaa
    22141 gtgcttactg gtatctatgc tcgcgatgcc ggcacgcttt tatggctggg gaaagaaacg
    22201 acatttaccg ggccgaaatc ttcccaggaa gccgggattg ggattatcca tcaggaactg
    22261 aacctgatcc cgcagttgac cattgccgaa aacattttcc tcggtcgtga gtttgttaat
    22321 cgctttggca aaattgactg gaaaaccatg tatgccgaag cggataaatt gctggctaaa
    22381 cttaacctgc gctttaaaag cgacaagctg gtgggcgatc tttccatcgg tgaccagcaa
    22441 atggttgaaa tcgccaaagt gctgagcttt gagtcgaaag tcatcattat ggatgaaccg
    22501 accgatgcgc tgaccgatac cgaaaccgaa tccctgttcc gcgtcatacg cgagctgaaa
    22561 tcgcaaggcc gcggtattgt ctatatctcc caccgcatga aagaaatctt cgagatttgc
    22621 gatgacgtta ccgtttttcg tgacgggcaa tttattgctg agcgcgaagt ggcatcactg
    22681 accgaagatt cgctgattga gatgatggtg ggccgcaagc tagaagatca atatccacac
    22741 ctggacaaag cgccgggaga tatccgcctg aaagtcgata atctctgcgg acctggcgtt
    22801 aacgatgtct cttttacttt acgcaaaggc gaaattcttg gcgtctctgg tttgatgggc
    22861 gcaggtcgta ccgaactgat gaaagtgctc tacggcgcac taccgcgcac cagcggttac
    22921 gtcaccctgg atgggcatga agtcgttacc cgttcaccgc aggatggcct ggcaagcggc
    22981 attgtgtata tctccgaaga ccgtaaacgt gacggtttag tgttgggcat gtcagtaaaa
    23041 gagaacatgt cgctgacagc gctgcgctac ttcagccgcg ctggcggcag tttgaagcat
    23101 gccgatgaac agcaggctgt gagtgatttc attcgtctgt ttaatgtgaa aacaccgtcg
    23161 atggaacagg caattggtct gctttccggt ggcaatcagc aaaaagtggc gattgcccgc
    23221 ggtctgatga cacgccccaa agtgttgatc cttgatgaac ctacccgtgg cgtagatgtc
    23281 ggcgcgaaaa aagagatcta tcaactgatt aaccagttca aagccgatgg cttgagcatc
    23341 attctggtgt catcggagat gccagaagta ttaggcatga gcgatcgcat catcgtcatg
    23401 catgaagggc atctcagcgg ggaatttact cgtgagcagg ccacccagga agtgttaatg
    23461 gctgccgctg tgggcaagct taatcgcgtg aatcaggagt aaaaaaatga caacccagac
    23521 tgtctctggt cgccgttatt tcacgaaagc gtggctgatg gagcagaaat cgcttatcgc
    23581 tctgctggtg ctgatcgcga ttgtctcgac gttaagcccg aactttttca ccatcaataa
    23641 cttattcaat attctccagc aaacctcggt gaacgccatt atggcggtcg ggatgacgct
    23701 ggtgatcctg acgtcgggca tcgacttgtc tgtaggttct ctgttggcgc taaccggcgc
    23761 agttgctgca tctatcgtcg gcattgaagt caatgcgctg gtggctgtcg ctgctgctct
    23821 cgcgttaggt gccgcaattg gtgcggtaac cggggtgatt gtagcgaaag gtcgcgttca
    23881 ggcgtttatc gctacgctgg ttatgatgct tttactgcgc ggcgtgacca tggtttatac
    23941 caacggtagc ccagtgaata ccggctttac tgagaacgcc gatctgtttg gctggtttgg
    24001 tattggtcgt ccgctgggcg taccgacgcc agtctggatc atggggattg tcttcctcgc
    24061 ggcctggtac atgctgcatc acacgcgtct ggggcgttac atctacgcgt tgggcggcaa
    24121 cgaagcggca acgcgtcttt ctggtatcaa catcaataaa atcaaaatca tcgtctattc
    24181 tctttgtggt ctgctggcat cgctggccgg gatcattgaa gtggcgcgtc tctcctccgc
    24241 acaacccacg gcggggactg gctatgagct ggatgctatt gctgcggtgg ttctgggcgg
    24301 tacgagtctg gcgggcggaa aaggtcgcat tgttgggacg ttgatcggcg cattaattct
    24361 tggcttcctt aataatggat tgaatttgtt aggtgtttcc tcctattacc agatgatcgt
    24421 caaagcggtg gtgattttgc tggcggtgct ggtagacaac aaaaagcagt aataacgact
    24481 acaggacatc ttgaatatga acatgaaaaa actggctacc ctggtttccg ctgttgcgct
    24541 aagcgccacc gtcagtgcga atgcgatggc aaaagacacc atcgcgctgg tggtctccac
    24601 gcttaacaac ccgttctttg tatcgctgaa agatggcgcg cagaaagagg cggataagct
    24661 tggctataac ctggtggtgc tggactccca gaacaacccg gcgaaagagc tggcgaacgt
    24721 gcaggactta accgttcgcg gcaccaaaat tctgctgatt aacccgaccg actccgacgc
    24781 agtaggtaat gctgtgaaga tggctaacca ggcgaacatc ccggttatca ctcttgaccg
    24841 ccaggcaacg aaaggtgaag tggtgagcca cattgcttct gataacgtac tgggtggcaa
    24901 aatcgccggt gattacatcg cgaagaaagc gggtgaaggt gccaaagtta tcgagctgca
    24961 aggcattgct ggtacatccg cagcccgtga acgtggcgaa ggcttccagc aggccgttgc
    25021 tgctcacaag tttaatgttc ttgccagcca gccagcagat tttgatcgca ctaaaggttt
    25081 gaacgtaatg cagaacctgt tgaccgctca tccggatgtt caggctgtat tcgcgcagaa
    25141 tgatgaaatg gcgctgggcg cgctgcgcgc actgcaaact gccggtaaat cggatgtgat
    25201 ggtcgtcgga tttgacggta caccggatgg cgaaaaagcg gtgaatgatg gcaaactagc
    25261 agcgactatc gctcagctac ccgatcagat tggcgcgaaa ggcgtcgaaa ccgcagataa
    25321 agtgctgaaa ggcgagaaag ttcaggctaa gtatccggtt gatctgaaac tggttgttaa
    25381 gcagtagttt taatcaggtt gtatgacctg atggtgacat aaatacgtca tcgacagatg
    25441 aacgtgtaat ataaagaaaa gcagggcacg cgccacccta acacggtggc gcattttatg
    25501 gacatcccga atatgcaaaa cgcaggcagc ctcgttgttc ttggcagcat taatgctgac
    25561 cacattctta atcttcaatc ttttcctact ccaggcgaaa ccgtaaccgg taaccactat
    25621 caggttgcat ttggcggcaa aggcgcgaat caggctgtgg ctgctgggcg tagcggtgcg
    25681 aatatcgcgt ttattgcctg tacgggtgat gacagcattg gtgagagcgt tcgccagcag
    25741 ctcgccactg ataacattga tattactccg gtcagcgtga tcaaaggcga atcaacaggt
    25801 gtggcgctga tttttgttaa tggcgaaggt gagaatgtca tcggtattca tgccggcgct
    25861 aatgctgccc tttccccggc gctggtggaa gcgcaacgtg agcgtattgc caacgcgtca
    25921 gcattattaa tgcagctgga atcaccactc gaaagtgtga tggcagcggc gaaaatcgcc
    25981 catcaaaata agactatcgt tgcgcttaac ccggctccgg ctcgcgaact tcctgacgaa
    26041 ctgctggcgc tggtggacat tattacgcca aacgaaacgg aagcagaaaa gctcaccggt
    26101 attcgtgttg aaaatgatga agatgcagcg aaggcggcgc aggtactgca tgaaaaaggt
    26161 atccgtactg tactgattac tttaggaagt cgtggtgtat gggctagcgt gaatggtgaa
    26221 ggtcagcgcg ttcctggatt ccgggtgcag gctgtcgata ccattgctgc cggagatacc
    26281 tttaacggcg cgttaatcac ggcgttgctg gaagaaaaac cattgccaga ggcgattcgg
    26341 tttgcccatg ctgccgctgc gattgccgta acacgtaaag gcgcacaacc ttccgtaccg
    26401 tggcgtgaag agatcgacgc atttttagac aggcagaggt gacgcttggc tacaatgaaa
    26461 gatgttgccc gcctggcggg cgtttctacc tcaacagttt ctcacgttat caataaagat
    26521 cgcttcgtca gtgaagcgat taccgccaaa gttgaagcgg cgattaaaga actcaattac
    26581 gcgccatcag ccctggcgcg tagcctcaaa ctcaatcaaa cacataccat tggcatgttg
    26641 atcactgcca gtaccaatcc tttctattca gaactggtgc gtggcgttga acgcagctgc
    26701 ttcgaacgcg gttatagtct cgtcctttgc aataccgaag gcgatgaaca gcggatgaat
    26761 cgcaatctgg aaacgctgat gcaaaaacgc gttgatggct tgctgttact gtgcaccgaa
    26821 acgcatcaac cttcgcgtga aatcatgcaa cgttatccga cagtgcctac tgtgatgatg
    26881 gactgggctc cgttcgatgg cgacagcgat cttattcagg ataactcgtt gctgggcgga
    26941 gacttagcaa cgcaatatct gatcgataaa ggtcataccc gtatcgcctg tattaccggt
    27001 ccgctggata aaactccggc gcgcctgcgg ttggaaggtt atcgggcggc gatgaaacgt
    27061 gcgggtctca acattcctga tggctatgaa gtcactggtg attttgaatt taacggcggg
    27121 tttgacgcca tgcgccaact gttatcacat ccgctgcgtc ctcaggccgt ctttaccgga
    27181 aatgacgcta tggctgttgg cgtttaccag gcgttatatc aggcagagtt acaggttccg
    27241 caggatatcg cggtgattgg ctatgacgat atcgaactgg caagctttat gacgccacca
    27301 ttaaccacta tccaccaacc gaaagatgaa ctgggggagc tggcgattga tgtactcatc
    27361 catcggataa cccagccgac ccttcagcaa caacgattac aacttactcc gattctgatg
    27421 gaacgcggtt cggcttagat ttacgctgtc ttttgatcaa attattacca tcggttgttt
    27481 tcagaagcat gaacattgct gctgaagcaa cagtaataat gcccatcgtg ataaacgtat
    27541 agtggaattg ttcgacagtc gttgtgcctt ccatcccttc ataaacgcga aggacggccg
    27601 cacttacagc aacgcctaaa ctaatcgaca gttgctgcgt gaccgccaga acactgttac
    27661 cgctgctggc gttgtcatcg gtcagatcgg caagtgtgat ggtattcatc gcggtaaatt
    27721 gcgtcgacat agccatccct aatataaaca acggcaagat cagcatccat atcgccattg
    27781 ccggtgattg caaagagaac tgagcgatca ttagcccaat aatcaccgtg atccccacta
    27841 acgtatggcg atagcccaga cgacgtaaga cttgggtaac catcgatttt gcaataatgg
    27901 aacctaacgc tgtcggtgcc atcatacagc cggcaataaa cgcctgataa ccaaatccta
    27961 cctgtaacat caatggcata aggaacggta cacaaccggt ccccagacgg gttgcaatat
    28021 tgcctacgat accgatcgag aaagtgcggg ttttaaataa atctaatgaa attaatgggt
    28081 ttggcgtgtg tcgcgcatgg agaatataga gaagcagtaa cccgatgctg gtgacaatta
    28141 ccgtcaaggc aatccagctg gcgacaatct tttccccgaa tagctctatt ccgcttgaga
    28201 agagaacaag gctgaggcca aacagcaaaa agccagtgat atcgaatctg cgtcgtgcgg
    28261 tggtgaaatt gggcatatgt ttgcgcgcgt aaagaaggcc cgcaatacct atggggatat
    28321 tgattaaaaa tatccagtgc caggttgccc aggtgaccag cacgccgcca agaacggggc
    28381 ctaaaattgg ccccaccaga cccggcatgg cgacaaaatt caatactgga agaagttcat
    28441 tacgaggata agcgcgcagt aaggccagcc gagcaacagg catcatcatt gcgccgccta
    28501 tcccctgaat aacccggaag acaaccagct gtggtagcga attagaaagt gcgcaggcca
    28561 gagaacccaa tgtgaacaga ctcacggcaa gggtaaaaat gcgacgcgta ccgaagcgat
    28621 cggctagcca tccgcttacc ggaataagca tcgccaccgt cagcgtataa ctgatgatgg
    28681 ctgattgcat cgcgagagga gaacgattaa ggctatgagc gattgcgggt aaggcggtat
    28741 tcagaatagt ggcatcaagt gcctgcatga agaaggccat cgccgcgatc cacggcaaac
    28801 ccgccatact gcgattcttt ttatcgctca ttcaatgtcc tgttatcggg ttgtcactta
    28861 tcaggtgagc gtagcagcgc ctgacaagct ttaaatgccg cgtcgccatc gctttggaca
    28921 atcgcatcga caatcgcctg gtgcagatcc agctttatca ctgtgtcgct ggtaattgac
    28981 gtgaagtaag tgtgataaac cgaatggaat agcgaggcga atgaggtcaa aaacggattg
    29041 gcgctcattt catagatatg ctcatgccag gccatatcga cttcgatcca gcgttcacgg
    29101 cgaaagttct cttttaatgc cgccatttcg gccattaacg tattgagatg cgccttctgt
    29161 tccgcggtgc caaccgttgc agccagtagg caagcttgcg gctccagaca gatacgcata
    29221 accagaaagt gatcgatgac ctgatgaaag ttctcttctg tcatccacca ggtaagcaat
    29281 tcctgatcaa gaaaattcca gtttgattgt ggcatgaccc gggtaccaat tcgcggtcgc
    29341 ggtaaaacca tcccttttgc cgttaacgtt ttgaccgctt cgcgtaccgc tgtacgactc
    29401 actccaaatt gctcgcccag ctcaatctca ccaggcaaaa tggtgccggg ttcatattca
    29461 ccttttaaga tgcgttgcgc cagcttctca gccagaacat acgaaaggtt tttctgtgca
    29521 gctaactgtt gtgcgcttaa aggcattact aatcttcctt tttcttttta ttcctcctta
    29581 gtatgccacc aggaagtgtg attacggttg caaaaacggc aaattgcttg ttttatggca
    29641 cattaacggg gcttttgct
//
LOCUS       DAZGAN020000052        29048 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGAN020000052 DAZGAN020000000
VERSION     DAZGAN020000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.052"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(116..1780)
                     /gene="glnS"
                     /locus_tag="ACHKYO_RS12900"
                     /old_locus_tag="pgaptmp_002580"
     CDS             complement(116..1780)
                     /gene="glnS"
                     /locus_tag="ACHKYO_RS12900"
                     /old_locus_tag="pgaptmp_002580"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HGU4429414.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPLL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1983..3929)
                     /gene="nagE"
                     /locus_tag="ACHKYO_RS12905"
                     /old_locus_tag="pgaptmp_002581"
     CDS             complement(1983..3929)
                     /gene="nagE"
                     /locus_tag="ACHKYO_RS12905"
                     /old_locus_tag="pgaptmp_002581"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HGU4429415.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4262..5062
                     /gene="nagB"
                     /locus_tag="ACHKYO_RS12910"
                     /old_locus_tag="pgaptmp_002582"
     CDS             4262..5062
                     /gene="nagB"
                     /locus_tag="ACHKYO_RS12910"
                     /old_locus_tag="pgaptmp_002582"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGU4429416.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5122..6270
                     /gene="nagA"
                     /locus_tag="ACHKYO_RS12915"
                     /old_locus_tag="pgaptmp_002583"
     CDS             5122..6270
                     /gene="nagA"
                     /locus_tag="ACHKYO_RS12915"
                     /old_locus_tag="pgaptmp_002583"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU4429417.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6279..7499
                     /gene="nagC"
                     /locus_tag="ACHKYO_RS12920"
                     /old_locus_tag="pgaptmp_002584"
                     /gene_synonym="nagR"
     CDS             6279..7499
                     /gene="nagC"
                     /locus_tag="ACHKYO_RS12920"
                     /old_locus_tag="pgaptmp_002584"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HGU4429418.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7547..8299
                     /gene="nagD"
                     /locus_tag="ACHKYO_RS12925"
                     /old_locus_tag="pgaptmp_002585"
     CDS             7547..8299
                     /gene="nagD"
                     /locus_tag="ACHKYO_RS12925"
                     /old_locus_tag="pgaptmp_002585"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HGU4429419.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8556..10220
                     /gene="asnB"
                     /locus_tag="ACHKYO_RS12930"
                     /old_locus_tag="pgaptmp_002586"
     CDS             8556..10220
                     /gene="asnB"
                     /locus_tag="ACHKYO_RS12930"
                     /old_locus_tag="pgaptmp_002586"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HGU4429420.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10601..10677
                     /locus_tag="ACHKYO_RS12935"
                     /old_locus_tag="pgaptmp_002587"
     tRNA            10601..10677
                     /locus_tag="ACHKYO_RS12935"
                     /old_locus_tag="pgaptmp_002587"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10635..10637,aa:Met,seq:cat)
     gene            10686..10770
                     /locus_tag="ACHKYO_RS12940"
                     /old_locus_tag="pgaptmp_002588"
     tRNA            10686..10770
                     /locus_tag="ACHKYO_RS12940"
                     /old_locus_tag="pgaptmp_002588"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10720..10722,aa:Leu,seq:tag)
     gene            10794..10868
                     /locus_tag="ACHKYO_RS12945"
                     /old_locus_tag="pgaptmp_002589"
     tRNA            10794..10868
                     /locus_tag="ACHKYO_RS12945"
                     /old_locus_tag="pgaptmp_002589"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10826..10828,aa:Gln,seq:ttg)
     gene            10903..10977
                     /locus_tag="ACHKYO_RS12950"
                     /old_locus_tag="pgaptmp_002590"
     tRNA            10903..10977
                     /locus_tag="ACHKYO_RS12950"
                     /old_locus_tag="pgaptmp_002590"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10935..10937,aa:Gln,seq:ttg)
     gene            10993..11069
                     /locus_tag="ACHKYO_RS12955"
                     /old_locus_tag="pgaptmp_002591"
     tRNA            10993..11069
                     /locus_tag="ACHKYO_RS12955"
                     /old_locus_tag="pgaptmp_002591"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11027..11029,aa:Met,seq:cat)
     gene            11118..11192
                     /locus_tag="ACHKYO_RS12960"
                     /old_locus_tag="pgaptmp_002592"
     tRNA            11118..11192
                     /locus_tag="ACHKYO_RS12960"
                     /old_locus_tag="pgaptmp_002592"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11150..11152,aa:Gln,seq:ctg)
     gene            11230..11304
                     /locus_tag="ACHKYO_RS12965"
                     /old_locus_tag="pgaptmp_002593"
     tRNA            11230..11304
                     /locus_tag="ACHKYO_RS12965"
                     /old_locus_tag="pgaptmp_002593"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11262..11264,aa:Gln,seq:ctg)
     gene            complement(11458..12633)
                     /gene="ubiF"
                     /locus_tag="ACHKYO_RS12970"
                     /old_locus_tag="pgaptmp_002594"
     CDS             complement(11458..12633)
                     /gene="ubiF"
                     /locus_tag="ACHKYO_RS12970"
                     /old_locus_tag="pgaptmp_002594"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HGU4429421.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDTAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVMLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12779..14203
                     /gene="miaB"
                     /locus_tag="ACHKYO_RS12975"
                     /old_locus_tag="pgaptmp_002595"
     CDS             12779..14203
                     /gene="miaB"
                     /locus_tag="ACHKYO_RS12975"
                     /old_locus_tag="pgaptmp_002595"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HGU4429422.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14356..15396
                     /locus_tag="ACHKYO_RS12980"
                     /old_locus_tag="pgaptmp_002596"
     CDS             14356..15396
                     /locus_tag="ACHKYO_RS12980"
                     /old_locus_tag="pgaptmp_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGU4429423.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15393..15860
                     /gene="ybeY"
                     /locus_tag="ACHKYO_RS12985"
                     /old_locus_tag="pgaptmp_002597"
     CDS             15393..15860
                     /gene="ybeY"
                     /locus_tag="ACHKYO_RS12985"
                     /old_locus_tag="pgaptmp_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGU4429424.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15950..16828
                     /gene="corC"
                     /locus_tag="ACHKYO_RS12990"
                     /old_locus_tag="pgaptmp_002598"
     CDS             15950..16828
                     /gene="corC"
                     /locus_tag="ACHKYO_RS12990"
                     /old_locus_tag="pgaptmp_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HGU4429425.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16853..18391
                     /gene="lnt"
                     /locus_tag="ACHKYO_RS12995"
                     /old_locus_tag="pgaptmp_002599"
     CDS             16853..18391
                     /gene="lnt"
                     /locus_tag="ACHKYO_RS12995"
                     /old_locus_tag="pgaptmp_002599"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HGU4429426.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18469..20346)
                     /locus_tag="ACHKYO_RS13000"
                     /old_locus_tag="pgaptmp_002600"
     CDS             complement(18469..20346)
                     /locus_tag="ACHKYO_RS13000"
                     /old_locus_tag="pgaptmp_002600"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HGU4429427.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPCAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            20801..21709
                     /gene="gltI"
                     /locus_tag="ACHKYO_RS13005"
                     /old_locus_tag="pgaptmp_002601"
     CDS             20801..21709
                     /gene="gltI"
                     /locus_tag="ACHKYO_RS13005"
                     /old_locus_tag="pgaptmp_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HGU4429428.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDEPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21879..22619
                     /gene="gltJ"
                     /locus_tag="ACHKYO_RS13010"
                     /old_locus_tag="pgaptmp_002602"
     CDS             21879..22619
                     /gene="gltJ"
                     /locus_tag="ACHKYO_RS13010"
                     /old_locus_tag="pgaptmp_002602"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HGU4429429.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            22619..23293
                     /gene="gltK"
                     /locus_tag="ACHKYO_RS13015"
                     /old_locus_tag="pgaptmp_002603"
     CDS             22619..23293
                     /gene="gltK"
                     /locus_tag="ACHKYO_RS13015"
                     /old_locus_tag="pgaptmp_002603"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HGU4429430.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23293..24018
                     /gene="gltL"
                     /locus_tag="ACHKYO_RS13020"
                     /old_locus_tag="pgaptmp_002604"
     CDS             23293..24018
                     /gene="gltL"
                     /locus_tag="ACHKYO_RS13020"
                     /old_locus_tag="pgaptmp_002604"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HGU4429431.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24136..25071
                     /gene="rihA"
                     /locus_tag="ACHKYO_RS13025"
                     /old_locus_tag="pgaptmp_002605"
     CDS             24136..25071
                     /gene="rihA"
                     /locus_tag="ACHKYO_RS13025"
                     /old_locus_tag="pgaptmp_002605"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HGU4429432.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQKC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            25155..26825
                     /gene="hscC"
                     /locus_tag="ACHKYO_RS13030"
                     /old_locus_tag="pgaptmp_002606"
     CDS             25155..26825
                     /gene="hscC"
                     /locus_tag="ACHKYO_RS13030"
                     /old_locus_tag="pgaptmp_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HGU4429433.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(26885..28336)
                     /gene="djlC"
                     /locus_tag="ACHKYO_RS13035"
                     /old_locus_tag="pgaptmp_002607"
     CDS             complement(26885..28336)
                     /gene="djlC"
                     /locus_tag="ACHKYO_RS13035"
                     /old_locus_tag="pgaptmp_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HGU4429434.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(28333..29040)
                     /locus_tag="ACHKYO_RS13040"
                     /old_locus_tag="pgaptmp_002608"
     CDS             complement(28333..29040)
                     /locus_tag="ACHKYO_RS13040"
                     /old_locus_tag="pgaptmp_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4429435.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         7151 a         7543 c         7329 g         7025 t
ORIGIN      
        1 aattaattcg cgaagaaaag cagaaaaaac gccgcagagt gcggcgtttg ggattgtttg
       61 ctatttcttg ccggatgcga cgtaaatgcc tcacccggcc tggtagaact taaaattact
      121 cgcctacttt cgcccaggta tcacgcagcc caacggtgcg gttaaatacc ggtttttccg
      181 ctgtggaatg gcggctgtcg aggcagaagt aaccttcacg ctcaaactgg aatgctttac
      241 ccgcaaccgc atctttcagc aacggttcag caaagccctg tttgatcacc agcgattccg
      301 ggttaatcac cgacaggaaa tcatccgcag caccagggtt cggcacgctg aacagacggt
      361 cgtacagacg gatttcaacc ggcagcgcat gtgccgcgct cacccagtga ataacacctt
      421 tgactttacg accatctgcc ggatctttgc ttaaggtgtc ggcgtcataa gtacagaaga
      481 tggtggtgat attgccttcg gcatctttct ccacgcgttc tgccttaatg acgtaagcat
      541 tacgcagacg cacttcttta cccagcacca gacgtttgta ctgcttgtta gcttcttcgc
      601 ggaaatcggc gcgatcgatc caaatctcac cgctaaacgg cacctggcgg ctacccattt
      661 ccggtttgtt cggatggttc ggcatggtaa ccatttcgcc ttcgccctga tagttttcga
      721 taaccagttt caccggatcg ataaccgcca ttgcgcgcgg cgcattttcg ttgagatctt
      781 cacggatgca ggattccagc gatgccatct caatggtgtt gtcctgcttg gtcacgccga
      841 tgcgtttgca gaactcacga atagaagcag cagtgtaacc acgacgacgc agaccggaaa
      901 tggtcggcat acgcgggtca tcccagcctt caacgtgctt gtcggtcacc agcaggttca
      961 gcttacgctt ggacatcaca gtgtattcca gattcaggcg cgagaattcg tactggcgcg
     1021 ggtgaacagg aatggtgatg ttgtccagca cccagtcgta caggcgacgg ttgtcctgga
     1081 actcaagcgt acacagagag tgcgtaatac cttccagggc atcgctgatg cagtgggtga
     1141 agtcgtacat cgggtagatg caccacttgt tgccagtctg gtggtgttca gcaaatttaa
     1201 tacggtacag caccggatcg cgcatcacga taaacggtga agccatgtcg attttcgcac
     1261 gcaggcaggc tttaccttct tcaaaaccac cggcacgcat tttttcgaac agcgccaggt
     1321 tctcttcaac gctgcggtcg cggtacgggc tgtttttacc cggttgcgtc agggtgccgc
     1381 ggtattcgcg gatctgttcc ggcgtcagtt catcaacgta cgccaggcct ttattgatca
     1441 gttcgatcgc ataggcgtgg agctgatcaa aataatcgga ggagtaacgg acgttaccag
     1501 accagtgaaa acctaaccac tctacgtcgt ttttgatcga ctcaacatac tcgatatctt
     1561 cttttaccgg gttagtgtcg tcgaaacgca ggttgcactg gcctttatag tcctgggcga
     1621 tcccgaagtt caggcagata gatttcgcat ggccaatatg cagatagcca ttcggctccg
     1681 gcgggaaacg ggtgtgtact gtggtgtgct taccactggc cagatcttca tcgatgatct
     1741 gacggataaa gttagtcggg cgggcttctg cctcactcat cgtggattcc tcaaagcgta
     1801 aacaacgtat aacggcgtat gatcttataa gtgggacagg ctgacaactg ttagtttttt
     1861 aaaattgata atctaacaag gattatgggg atgattgatg aaaaaaaaag cggcagagaa
     1921 gatctccacc gcttgtcatt gttggatgcg acgctcaagc gtcgcatcag gcataaagca
     1981 gattactttt tgatttcata cagcggtgtt tgacccgcca caacatggcc ctgagcttta
     2041 atgatcaagc cactgaaatc gtcgatattg ctgcaaacca ccgggctaat catcgagcgg
     2101 gcgttagcgt tcaggtaatc cagatccatt tccagaatcg gttgccctgc gcttacctgc
     2161 gcaccctctt ccaccagacg tttaaagcct ttaccttcca gcgctacggt gtcgataccc
     2221 atatggacga cgatctccgc gcctttttcg gtttccaggc agaacgcgtg gttggtgttg
     2281 aagattttca cgattgtccc tgcggctggt gatacgacga ttttatctgt cggtttcacc
     2341 gccacaccgt cacccaccgc tttgctggcg aatgcttcgt caggaacctg atccagtgcc
     2401 acgacatcac cggtaatcgg cgataccagc tccgcgatag ataccgcgtt tggtacagcc
     2461 tgcggttttg ctacaggcgc ggcagttgcc ggagttgctt cagctgacgc agcggctacc
     2521 ggaccacggg caacgacttt cttcatcgca tcgccgatgg attctgcttt cgcgccaaca
     2581 atcacctgaa tagtctgttt gttcagtttc actacaccag aagcacccag acgtttacac
     2641 atcgtatcgt tgacgcgggc agagtcagcc acggtcaggc gcagacgggt gatacaggcg
     2701 tcaattgctt tcaggttgtc agtgccgcca accgcagcaa tatagttggt cgccagttga
     2761 ttgaggcctt cttcagtgtt gctgttggct tcttcagtaa cgatctcgtc ttctttatct
     2821 tcacgacccg gcgttttcag gttgaacatg cggataacca aactgaacac cacgaagtag
     2881 atagcgaaga agacaacacc cataaccagc agcatccaga cgttctggct ggcggccggc
     2941 aggttataca tcaacgcgta gtcgatagcc cccgcagaga aagagaagcc agcatggata
     3001 cccagcagtg ttgccacaaa caggctgata ccggtcagca gtgcgtgcag gaggtacagc
     3061 agcggtgcaa ggaacatgaa caggaattcc agcggctcag tcacaccggt caggaacgca
     3121 gtaacagcaa cagaaagcag catcccgcca accatcggac gacgctcttt cggtgctgcg
     3181 aagtacatcg ccagcgccgc acccggcaga ccgaacatca taatcgggaa gaagccggac
     3241 atgaacatcc ccgcggtgcc gtcaccggca tagaagcggt tgatatcacc gtggaaaacc
     3301 gtacccgccg cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt cagcacctga
     3361 tgcagaccgg ttgggatcag cagacggttg atgaaaccaa agataccgga acccagcgcg
     3421 cccgcagaaa cgatccactc gcctcctgca tggatagcgt gctgtaccgg cggccagacg
     3481 taaccaaaaa tggccgccag caccaggcag aagaagccgg tggcaatcgg cacaaagcgt
     3541 ttgccgccga agaaactcag gaagtccggc agtttaatat cggaccaacg gttataggct
     3601 gcgccaccaa ccagaccggt aatgataccc gccagtacac ccatgttaat ttctgggttg
     3661 atggtcacca tcgctttggt taacacaaag taacctaccg cacccgccag cgccgccgca
     3721 cctgcgctgt ctttcgacca gctggatgcc acaccgatgg cgaagattaa tgcgaggtta
     3781 tcaaaaatcg caccgcccgc ctgggcaata aacgcaacgt taagtaaatc tggctgaccg
     3841 aatcgcagca acagtgccgc caccggcagc accgcgatag ggagctgtaa cgccctaccg
     3901 agtcgctgga aaaaacctaa aatattcatc ttattccccc tacgagaacc ctatttggct
     3961 cgtttaaagc cgtattttta ttttgctgca aattgtactg ccgatgttct gtaatcagat
     4021 tgttagatca tctgctacag agtgtgtgaa aatttaattc gtatcgcaaa ttaaacgcgt
     4081 gtcttttgtg agttttgtca ccaaatatcg ttatcatcac tcccttttac tggctaaagc
     4141 agaaaactta ttttatcatt caaaaaatca ggtcggattg acgcctgtct gcgcaaatcc
     4201 aggttacgct taaagatgcc taatccgcca acggcttaca ttttacttat tgaggtgaat
     4261 aatgagactg atccccctga ctaccgctga acaggtcggc aaatgggctg ctcgccatat
     4321 cgtcaatcgt atcaatgcgt tcaaaccgac tgccgatcgt ccgtttgttc tgggcctgcc
     4381 gactggcggc acgccgatga ccacctataa agcgttagtc gaaatgcata aagcaggcca
     4441 ggtcagcttt aagcacgttg tcaccttcaa catggacgaa tatgtcggtc tgccgaaaga
     4501 gcatccggaa agctactaca gctttatgca ccgtaatttc ttcgatcacg ttgatattcc
     4561 agcagaaaac atcaaccttc tcaacggcaa cgccccggat atcgacgccg aatgccgcca
     4621 gtatgaagaa aaaatccgtt cttacggaaa aattcatctg tttatgggcg gtgtaggtaa
     4681 cgacggtcat attgcattta acgaaccggc gtcttctctg gcttctcgta ctcgtatcaa
     4741 aaccttgact catgacactc gcgtcgcgaa ctctcgtttc tttgataacg atgtcaatca
     4801 ggtgccaaaa tatgccctga ctgtcggtgt tggtacattg ctggatgctg aagaagtgat
     4861 gattctggtg ctgggtagcc agaaagcact ggcactgcag gctgcagttg aaggttgcgt
     4921 gaaccacatg tggaccatca gctgtctgca actgcatcca aaagcgatca tggtgtgcga
     4981 tgaaccttcc accatggagc tgaaagttaa gactttaaga tatttcaatg aattagaagc
     5041 agaaaatatc aaaggtctgt aattgttatc cctgccctgg ctccttgctc agggcaatat
     5101 tttttaaaat cgggggtcag aatgtatgca ttaacccagg gccggatctt taccggccac
     5161 gaatttcttg atgaccacgc ggttgttatc gctgatggcc tgattaaaag cgtctgtccg
     5221 gtagcggaac tgccgccaga gatcgaacaa cgttcactga acggggccat tctctccccc
     5281 ggttttatcg atgtgcagtt aaacggctgc ggcggcgtac agtttaacga caccgctgaa
     5341 gcggtcagcg tggaaacgct ggaaatcatg cagaaagcca atgagaaatc aggctgtact
     5401 aactatctgc cgacgcttat caccaccagc gatgagctga tgaaacaggg cgtgcgcgtt
     5461 atgcgcgagt acctggcaaa acatccgaat caggcgttag gtctgcatct ggaaggtccg
     5521 tggctgaatc tggtaaaaaa aggcacccat aatccgaatt ttgtgcgtaa gcctgatgcc
     5581 gcgctggtcg atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac cctggcaccg
     5641 gaaatggttc ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt ggtttctgcc
     5701 ggtcactcca acgcgacgtt gaaagaagca aaagccggtt tccgcgcggg gattaccttt
     5761 gccacccatc tgtacaacgc gatgccgtat attaccggtc gtgaaccggg cctggcgggc
     5821 gcgatcctcg acgaagctga catttattgc ggtattattg ctgatggcct gcatgttgat
     5881 tacgccaaca ttcgtaacgc taaacgtctg aaaggcgaca aactgtgtct ggttaccgac
     5941 gccaccgcgc cagcaggtgc caacattgaa cagttcattt ttgcgggtaa aacaatatac
     6001 taccgtaacg gactttgtgt ggatgagaac ggtacgttaa gcggttcatc cttaaccatg
     6061 attgaaggcg tgcgtaatct ggtcgaacat tgcggtatcg cactggatga agtgctgcgt
     6121 atggcgacgc tctatccggc gcgtgcgatt ggcgttgaga aacgtctcgg cacactcgcc
     6181 gcaggtaaag tagccaacct gaccgcattc acacctgatt ttaaaatcac caagaccatc
     6241 gttaacggta acgaggtcgt aactcaataa gagaaagtat gacaccaggc ggacaagctc
     6301 agataggtaa tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat cgcctgattg
     6361 accagtacgg gccaatctcg cggattcaga ttgccgagca aagccagctt gcccccgcca
     6421 gcgtaaccaa aattacgcgt cagcttatcg aacgcgggct gatcaaagaa gttgatcagc
     6481 aggcctccac cgggggccgc cgcgctatct ccatcgtcac cgaaacccgc aatttccacg
     6541 caatcggcgt acggcttggt cgtcacgacg ccaccatcac cctgtttgat ctcagcagca
     6601 aagtgctggc agaagaacat tacccgctgc cggaacgtac ccagcaaacg ctggaacatg
     6661 ccctgttgaa tgccattgct cagtttattg atagctacca gcgcaaactg cgcgagctga
     6721 tcgcgatttc ggtgatcctg ccagggcttg ttgacccgga cagcggcaaa attcattaca
     6781 tgccgcatat tcaggtagaa aactgggggc tggtagaagc actggaagaa cgttttaaag
     6841 tgacctgttt cgttggtcac gatatccgta gtctggcgct ggcggagcac tacttcggtg
     6901 caagtcagga ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc ggggccggga
     6961 ttatctctaa cgggcgcatt tttattggcc gcaacggcaa cgtcggtgaa attggccata
     7021 ttcaggtcga accgctgggt gaacgctgcc actgcggcaa ctttggctgc ctggaaacta
     7081 tcgctgccaa cgccgctatt gaacaacggg tgttgaatct gttaaagcag ggctaccaga
     7141 gccgcgtgcc gctggacgac tgcaccatca aaactatctg caaagccgcg aacaaaggcg
     7201 atagcctggc ctcggaagtg attgagtatg tcggtcgtca tctgggcaaa accatcgcca
     7261 ttgctatcaa cttatttaat ccgcaaaaaa ttgttattgc cggtgaaatc accgaagccg
     7321 ataaagtgct gctccctgct attgaaagct gcattaatac ccaggcgctg aaggcgtttc
     7381 gcactaatct gccggtggta cgttctgagc tggatcaccg ctcggcaatc ggcgcttttg
     7441 cgctggtaaa acgcgccatg ctcaacggta ttttgctcca gcatttgctg gaaaattaat
     7501 gtgcttttat agtggcgctt attgttgtca atattctggg tagtccatga ccattaaaaa
     7561 tgtaatttgc gatatcgacg gcgtgctgat gcacgataac gtcgccgtac cgggtgcagc
     7621 ggaatttttg cacgggatta tggataaagg cctgccgctg gtgttgctga ccaactatcc
     7681 ttcgcagacc gggcaagatc tggcgaaccg ctttgccact gccggtgtcg atgtgccgga
     7741 tagcgtgttt tatacctctg cgatggcgac tgccgatttc ctgcgtcgcc aggaaggcaa
     7801 gaaagcgtat gtggtgggcg aaggcgcact gattcatgaa ctgtacaaag ccggtttcac
     7861 tattaccgat gtgaaccctg attttgtgat tgttggcgaa acgcgttcct acaactggga
     7921 catgatgcat aaagcggcct atttcgtcgc taacggtgca cgctttatcg ccaccaaccc
     7981 ggacacccac gggcgcggtt tttatcccgc ttgtggcgcg ttgtgtgcag ggattgagaa
     8041 aatctccggg cgcaaaccgt tctatgttgg taagcccagc ccgtggatca tccgcgcggc
     8101 attaaacaaa atgcaggcgc attcggaaga aacggtgatt gtcggcgata acttgcgtac
     8161 cgatattctg gcgggcttcc aggcgggtct ggagacgatt ctggtgcttt ctggtgtttc
     8221 atcgctcgac gatatcgaca gtatgccgtt ccgcccaagc tggatttacc cgtcggtcgc
     8281 tgaaatcgac gttatctgaa tagcaaccac gtctcagggc gtggtttctt atttttaccc
     8341 atcattaaaa tcatcccatt caataaaact cgccaaaaat tatcgatcca tcaataaata
     8401 catcaccaca ccatcccctt tttcacaaat cagcaatcac cattacgttt tttatttttg
     8461 cactgccaaa tcgcttgcgc acggtgctgc tttgcggcat ttttttaaac aagcaaacac
     8521 aacaagcaac aaataccagg ttaacggaga aggttatgtg ttcaattttt ggcgtattcg
     8581 atatcaaaac agacgcagtt gagctgcgta agaaagcact cgagctgtca cgcctgatgc
     8641 gtcatcgtgg cccggactgg tccggtattt atgccagcga taacgccatt ctcgctcacg
     8701 aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc tctctacaac caacaaaaaa
     8761 cccacgtact ggcggtaaac ggtgaaatct acaaccacca ggcattgcgc gctgaatatg
     8821 gcgatcgtta tcagttccag acagggtctg actgtgaagt gatcctcgcg ctgtatcagg
     8881 aaaaagggcc ggaatttctc gacgacttgc agggcatgtt tgcctttgcc ctgtacgaca
     8941 gcgaaaaaga tgcctacctg attggtcgcg accatctggg gatcatccca ctgtatatgg
     9001 gctatgacga acacggtcag ctgtatgtgg cctcagaaat gaaatcgctg gtgccagttt
     9061 gccgcacgat taaagagttc ccggcgggga gctatttgtg gagccaggac ggcgaaatcc
     9121 gttcttatta tcatcgcgac tggttcgact ttgatgcagt gaaagataac gtgaccgaca
     9181 aaaacgagct gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg tctgatgtgc
     9241 cttacggtgt gctgctttct ggtggtctgg attcctcaat tatttccgct atcaccaaga
     9301 aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg ccgcagttac
     9361 actcctttgc tgtaggtctg ccgggttcac cggatcttaa agcagcccag gaagtggcaa
     9421 accatctggg cacggtgcat cacgaaattc acttcactgt acaggaaggt ctggatgcca
     9481 tccgcgacgt gatttaccac atcgaaactt atgatgtgac cactattcgc gcttcaacac
     9541 cgatgtattt aatgtcgcgt aagatcaagg cgatgggcat taaaatggtg ctgtccggtg
     9601 aaggttctga tgaagtgttt ggcggttatc tttacttcca taaagcgccc aacgctaaag
     9661 aactgcatga agagacggtg cgtaaactgc tggccctgca tatgtatgac tgcgcgcgcg
     9721 ccaacaaagc gatgtccgcc tggggcgtgg aagcgcgcgt tccgttcctc gacaaaaaat
     9781 tccttgatgt ggcgatgcgt attaacccac aggataaaat gtgcggtaac ggcaaaatgg
     9841 aaaaacacat cctgcgtgaa tgttttgagt catacctgcc tgcaagcgtg gcctggcggc
     9901 agaaagagca gttctccgat ggcgtcggtt acagttggat cgacaccctg aaagaagtgg
     9961 cggcgcagca ggtttctgat caacagctgg aaactgcccg cttccgcttc ccgtacaaca
    10021 cgccgacgtc caaagaagcg tatctgtacc gggagatctt tgaagaactg ttcccacttc
    10081 cgagcgccgc tgagtgcgta ccgggcggtc cttccgtcgc gtgttcttcc gctaaagcga
    10141 ttgagtggga tgaggcgttc aagaaaatgg acgatccgtc tggtcgtgcg gttggcgttc
    10201 accagtcggc gtataagtaa gaaatgcgaa acagccccgg tgaaatccgg ggctgtttca
    10261 gttattgagg tgcttgttaa ttctgatatg cagtaaggaa atgttccggg tttgccacat
    10321 cactgtttga caactcatca ctgtaggcca gtatttcata gattaatttc ccaccactat
    10381 tgttgaattc cgaatttcat cctgtacgac aaaagaatgg ctgtagggtg gggaaaaaac
    10441 acaaagtaaa caataattga cgaatatagc gccacgctgt tcgcaaccta accaaacagt
    10501 cactttcgag caattttcct tgaaaaagag gttgacgctg caaggctcta tacgcataat
    10561 gcgccccgca acgccgataa ggtatcgcga aaaaaaagat ggctacgtag ctcagttggt
    10621 tagagcacat cactcataat gatggggtca caggttcgaa tcccgtcgta gccaccatct
    10681 tttttgcggg agtggcgaaa ttggtagacg caccagattt aggttctggc gccgcaaggt
    10741 gtgcgagttc aagtctcgcc tcccgcacca ttcaccagaa agcgttgtac ggatggggta
    10801 tcgccaagcg gtaaggcacc ggtttttgat accggcattc cctggttcga atccaggtac
    10861 cccagccatc ttcttcgagt aagcggttca ccgcccggtt attggggtat cgccaagcgg
    10921 taaggcaccg gtttttgata ccggcattcc ctggttcgaa tccaggtacc ccagccatcg
    10981 aagaaacaat ctggctacgt agctcagttg gttagagcac atcactcata atgatggggt
    11041 cacaggttcg aatcccgtcg tagccaccaa attctgaatg tgtcgaatac gttcggcaaa
    11101 ttcaaaaacc aatttgttgg ggtatcgcca agcggtaagg caccggattc tgattccggc
    11161 attccgaggt tcgaatcctc gtaccccagc caatttattc aagacgctta catcgtaagt
    11221 gcgcccagtt ggggtatcgc caagcggtaa ggcaccggat tctgattccg gcattccgag
    11281 gttcgaatcc tcgtacccca gccacattaa aaaagctcgc ttcggcgagc tttttgcttt
    11341 tctgcgttca ttcaatgtcg aatgcgatgt tgacacgtct tatccttcaa tgtcggatgc
    11401 gacgctgccg cgtcttatcc gacctacggt tggcacgcat ccggcaatgt tgtaaggcta
    11461 caaccctaac gcatatttca gcgcctgacg tttcaacacg ccagcacgct ctgccgccat
    11521 taacccgaga ttacgcacaa aacgcagcgg cggcagatta ttactgaatc cggcataaaa
    11581 cagatccata ccactttgca taatgaagtt atccgccatg cgccgcatct ggtaacgctt
    11641 gaggacagga taactggccc acgcttcacc gtagctgcgg gcattcacca gaacatcaat
    11701 cagggcatcg acatcacgat aaccaagatt caccccctgc cccgccagcg gatggatggt
    11761 atgcgcggca tcgcccacca gcgcaagccc cggctgcaca tactgcaacg catgtcggcg
    11821 cgtcagcgga aacgcaccag cggcaagcgg tgtaacgtaa cccagacgcg acgggaaatg
    11881 cttcgcgatt tccgcctgga gttgcgccat attcatattc tgcaactggc gaatacgtac
    11941 cggagagtca taccacacca gcgatgccca gttatcaaac aacggcagaa acgcacgtgg
    12001 tccgtccggg gtaaattgct gccaggtgct gtcgccggga tcgttctcac actgtacgct
    12061 aatcaacata cacgactgcg catactgcca ggcatgaacg ccaattcccg ccatctgccg
    12121 cacctgcgaa tttgcgccgt cagcaccaat caccagcttc gcgcgaatca cttcaccgcc
    12181 cttcagttcc agctcctgaa gatcattatc tcgatgtaat gcaatcagcg agcctggcac
    12241 acgtaacatt actttcggat gcgcttccag cgcctgccac aatgcctgtt gcaggacggt
    12301 gttttccacc atatagccaa gcagcggcag cttaagttca gcggtgtcaa acatcacatg
    12361 cgccgtttcc cactcccacg tttccagtct gcggtaagga tggcaacgca tagcctgtac
    12421 tgcatcccag acccctaacc ctttaagcaa tgataccgat gcggcgctga tcgccgagat
    12481 ccgcacgtcc ggttggctat cagcgacaaa cggtgcaggt tctgcgtgct cgatcaccgt
    12541 taccgaaaat ccgtgctgtg ccagccccag cgccagtgcg ccgccgacca ttcctccgcc
    12601 gacaatggca atttccgttg gttgatttgt catggtcaat catcctgtta gcaatatgct
    12661 ttaagtttac aggattttta cccccaaggg ctgataacgc tcatactggt cacaacggca
    12721 gcaaagcatt acactatgcg ccctgcattc ctggctacta tttcgcaaga gcaagtcgat
    12781 gaccaaaaaa ctccatatta aaacctgggg ctgtcagatg aacgagtacg attcatcgaa
    12841 gatggccgat ctgctggatg ccacccacgg ctatcaactg accgacgtgg cggaagaagc
    12901 ggatgtgctg ctgctgaaca cctgctcaat ccgcgagaag gctcaggaaa aagtcttcca
    12961 tcagttgggt cgctggaaac tgttaaaaga gaagaatcca gacctgatta tcggcgtcgg
    13021 tggctgcgtg gcatcgcaag aaggcgagca cattcgccag cgcgcccact atgtcgatat
    13081 tatttttggg ccgcaaacgc tgcaccgtct gccggagatg atcaactccg tgcgcggcga
    13141 ccgcagcccg gttgtagata tcagcttccc ggaaatcgag aagtttgacc gtctaccgga
    13201 accgcgcgcc gaagggccga ccgcgtttgt ctccatcatg gaaggctgca ataaatattg
    13261 tacctactgc gtggtgcctt acacccgtgg tgaagaggta agccgtccgt ctgacgatat
    13321 tctgtttgag attgcccagc ttgctgctca gggcgtgcgt gaagtcaacc tgctcggtca
    13381 gaacgtgaac gcctggcgtg gtgaaaacta cgacggcacc accggatcgt ttgccgatct
    13441 gctgcgtctg gttgctgcga tcgacgggat cgatcgtatt cgctttacca ccagccatcc
    13501 gatcgaattc accgacgata tcatcgaagt gtaccgcgac acgcctgagc tggtgagttt
    13561 cctgcatctg ccggtacaga gcggttccga tcgcattctg aacctgatgg gccgtaccca
    13621 tacggcgctg gagtacaaag cgatcatccg taaactgcgt gcggcgcgtc cagatattca
    13681 gatcagttct gacttcattg ttggcttccc tggcgaaacc accgaagact tcgagaaaac
    13741 gatgaagctg attgccgacg tcaatttcga catgagctac agctttatct tctctgcacg
    13801 tccgggtaca ccagccgccg atatggttga tgatgttccg gaagaagaga agaagcaacg
    13861 tctgtatatt ctgcaagaac gtatcaatca gcaagcgatg gcatggagcc gccggatgct
    13921 cggcaccacc cagcgtattc tggtagaagg gacttcgcgt aagagcatca tggagctttc
    13981 cgggcgtacc gaaaataacc gcgtggtcaa cttcgagggc acaccggata tgatcggtaa
    14041 attcgtcgat gtagaaatta ccgacgtcta cccgaactct ctgcgcggta aagtggtgcg
    14101 tactgaagat gaaatgggtc tgcgcgtggc agaaacaccg gaatcggtga ttgcccgtac
    14161 ccgcaaagaa aacgaccttg gcgtgggtta ttatcagccg taattctcag gcccgccgtt
    14221 ccggtgggcc ttgttttttc tctctttatc cccatcttta tcgcaatgct tgcgcctttt
    14281 tcctgtggcg tgaataattt ccttataggc cggtgaatgt tcagcttccg gcgacataca
    14341 gataagagga acggtttgaa catagacact cgcgaaatca ccctggagcc agcagacaac
    14401 gcgcgtctgt tgagcctgtg cggcccgttt gatgacaaca tcaagcagct cgaacgccgt
    14461 ctcggcatcg agatcaatcg ccgcgacaac cactttaaac tgaccggccg tccgatttgc
    14521 gtcaccgctg cggcagacat tctgcgtagc ctgtatgtcg atacagcccc gatgcgcggt
    14581 cagattcagg atatcgaacc ggaacagatc caccttgcga ttaaagaagc gcgagtactg
    14641 gagcaaagtg cggagagcgt gccggagtac ggcaaagcgg tcaatatcaa aaccaaacgc
    14701 ggcgtaatta agccgcgcac gccaaaccag gcgcagtaca tcgccaatat tctcgaccat
    14761 gacattacct tcggcgttgg cccggcgggt acgggtaaaa cctacctggc agtggctgcg
    14821 gcagttgatg ccctggagcg tcaggagatt cgccgtattc tgctgactcg tccggcggtc
    14881 gaagccggtg agaaactggg cttcctgcct ggcgatttaa gccagaaagt agacccgtat
    14941 ctgcgcccgc tgtacgacgc gctgtttgaa atgctgggct ttgagaaagt tgagaaactg
    15001 attgagcgca acgttattga agtcgcgccg ctggcctata tgcgtggtcg tacgctgaac
    15061 gacgcgttta tcattctcga tgagagccag aacaccacca tcgaacagat gaagatgttc
    15121 ctgacccgta tcggttttaa ctcaaaagcg gttatcaccg gcgacgtcac acagatcgac
    15181 ctgccgcgta atactaaatc aggcttacgt cacgccatcg aagtgctggc cgatgtcgaa
    15241 gagatcagct ttaacttctt ccacagcgaa gacgtggttc gtcaccctgt ggtggcgcgt
    15301 atcgttaacg cctatgaagc ctgggaagaa gccgaacaaa aacgaaaagc ggcgctggcg
    15361 gcagaacgca agcgcgaaga acaggaacaa aaatgagtca ggtgatcctc gatttacaac
    15421 tggcatgtga agataattcc gggttaccgg aagagagcca gtttcaaaca tggctgaatg
    15481 cggtgatccc gcagtttcag gaagaatcgg aagtgactat tcgcgtggtc gataccgccg
    15541 aaagccacag tctgaatctg acctatcgcg gtaaggataa gccgaccaac gtgctctcct
    15601 tcccgtttga agtgccgcct ggcatggaaa tgtcgctact gggcgatctg gttatctgcc
    15661 gtcaggtggt tgagaaggaa gctcaggagc aaggcaaacc actggaggcg cactgggcgc
    15721 atatggtggt gcacggcagt ctgcatttgt taggttacga tcacatcgaa gatgacgaag
    15781 cagaagaaat ggaagccctc gaaacagaga ttatgcttgc tctgggctat gaggatccgt
    15841 acattgccga gaaagaataa tttgccaggc atttgactgg cagacgtccc cgccgttggt
    15901 gattaacgac ggcgttttga ttaactaaca tgagaaccca tacgacgcca tgagcgacga
    15961 caattcacac agtagtgaca cgataagcaa caagaaggga tttttctccc tgttactcag
    16021 ccaacttttc cacggtgaac cgaaaaaccg tgacgaactg ctggcgctga tccgtgattc
    16081 cgggcagaac gaccttatcg acgaagatac gcgcgatatg ctcgaagggg tgatggacat
    16141 cgcagaccaa cgcgttcgcg acatcatgat cccccgctcc cagatgatta ccctgaaacg
    16201 caaccagacg ctggacgaat gccttgatgt catcatcgag tccgcccact cacgtttccc
    16261 ggtgattagc gaagacaaag atcacattga agggattctg atggcgaaag acttgctgcc
    16321 gtttatgcgc agcgatgctg aagccttcag catggacaaa gtgttacgtc aggcggttgt
    16381 cgttcctgaa agtaagcgcg tagaccggat gctgaaagag tttcgctctc agcgttacca
    16441 catggcgatc gttattgacg aattcggtgg ggtttccggt ctggtgacca ttgaagacat
    16501 cctggaactg attgttggtg agattgaaga cgagtatgac gaagaagatg atatcgactt
    16561 ccgtcagctg agtcgtcata cctggaccgt gcgcgcactg gcttccattg aagacttcaa
    16621 cgaagcgttc ggcacccact ttagcgatga agaagtcgac actatcggtg gtctggtgat
    16681 gcaggcgttt gggcatcttc cggcgcgtgg cgaaactatc gacatcgacg gttaccagtt
    16741 caaagtggcg atggccgata gtcggcgtat tattcaggtt catgtcaaaa tcccggatga
    16801 ctcaccccag ccaaagctgg atgaataaaa ccgaaactgg atagataact acatggcttt
    16861 tgcctcatta attgaacgcc agcgcattcg cctgctgctg gcgttattat tcggtgcctg
    16921 cggaacgctg gccttctctc cttacgatgt ctggcctgcg gcgattgttt cgctgatggg
    16981 gcttcaggcg ctgaccttta accgccgccc gctccagtct gccgctattg gcttctgctg
    17041 gggatttggc ctctttggca gcggtattaa ctgggtctat gtcagcatcg cgacctttgg
    17101 cgggatgcct ggcccggtta acatcttcct ggtggtgctg ctggcggcgt atttgtcgct
    17161 gtataccgga ctgtttgctg gcgtgctgtc gcgcctgtgg ccgaaaacca cctggctgcg
    17221 cgtggcgatt gccgcccctg ctctctggca agtgaccgag tttctgcgcg gttgggtgct
    17281 gactggcttc ccgtggttac agttcggcta tagccagatt gatggcccgt taaaagggct
    17341 ggcaccaata atgggcgtgg aagccattaa cttcctgctg atgatggtta gcggcctgct
    17401 ggcactggcg ttagtcaaac gcaactggcg tccgctggtg gtggccgtcg tgctgtttgc
    17461 ccttcccttc ccgctgcgtt acatccaatg gtttaccccg caaccggaga aaaccattca
    17521 ggtttcgatg gttcagggcg atattccgca atcactgaaa tgggacggag accagcttct
    17581 taatacgctg aagatttact acaacgcaac ggcaccgctg ctgggcaaat catcgttgat
    17641 tatctggccg gagtcggcga taaccgatct ggaaattaat cagcaaccgt tcctcaaagc
    17701 actggacggt gagttgcgtg ataaaggtag ctcgctcgta accgggattg tcgacgcgcg
    17761 tctcaataag cagaaccgct acgataccta caacaccatc atcacactgg gtaaaggtgc
    17821 accgtacagc tacgaatcag ccgatcgcta taacaaaaac catctggtgc cgtttggcga
    17881 gtttgtcccg ctggagtcga ttctgcgtcc gttagcaccg ttctttgatc tgccgatgtc
    17941 gtcgttcagc cgtgggccat atatccagcc gccgctgtcg gtaaatggta ttgagcttac
    18001 tgcggccatt tgctacgaga tcattcttgg cgagcaagtg cgcgataact tccgcccgga
    18061 taccgactat ctgctgacta tctccaacga tgcgtggttt ggtaaatcta ttggtccatg
    18121 gcaacacttc cagatggcgc gaatgcgtgc gctggagctg gcgcgcccac tgttgcgcag
    18181 caccaacaac ggcattacgg cggtgattgg cccgcagggt gagattcagg cgatgatccc
    18241 gcagttcacc cgcgaggtgt taaccactaa tgtgacgccg accaccggac tcacaccata
    18301 cgcacgtacc ggcaactggc cgctgtgggt gctgacggca ctgtttggtt ttgccgctgt
    18361 gctgatgagt ctgcgagcac gtaaacgttg atgtgcctga tgtatcctgc atgagcaaaa
    18421 cgtactttct cattgagctt tggggcatgg ttatcatgcc cctttttctt acggttcagg
    18481 gatacaagcc acattgccat attggggatg acgaataatt gcctgcggat caagcgagcg
    18541 cgtcctgtcc agttggatca acccgttctt cgccataaca aatatttttc taccatccgc
    18601 tgaaaaacga aggtgattaa cgtagctggc aggataaacg ccaataatct gcccggcatt
    18661 aatattgagc aggatgacat aaccttgcag gccatatgac accaacgcta aatactgacc
    18721 gtccgggcta ataccctcaa cggaataaac gctcctgcta caccattgcg tcataatgct
    18781 ggcttgttga tcccctacgc ttatacgtga aatcccctgg ctgtcggcaa aaattacatc
    18841 cccgttttca ggtcgaaaac gcatcgccga tatcgttcct cgcgcatcac tagccggata
    18901 gctttctttc tgatgcgtga tggtattgat gcgtacaatg tcacctctga ccataccggg
    18961 tatcaacaat attttttcat ccggggaaag agccataacc caggcacaag gtttattact
    19021 cgtccccgtc ccgtcgtatt tctccagcgg gatatccgcc acatgctgat aagtcaccag
    19081 atcaatcgct gacacactat tactggtgcg attcatcacc caggcttttg taccatcacg
    19141 cgagaggata aggctgtcag gatgtgcacc ggtggtaatc gactgaataa tctcccctgt
    19201 cgccacgtta atgaccgata acgcattcgc ttcaaatgaa gtcgcataaa tcaacttttc
    19261 atccgggctt aatgccagcg atctgacgcc ctgatcttta cagccaccac aatgtttgac
    19321 tgacttttct ttcgctatgg gcgctgtaaa ggtatgcagg attttctttt gtccgaggtc
    19381 gaaaacaaca atgctgttgg tgttttcaat tgcggcatac aacatattac catcccggct
    19441 gatcgccata tcatggatat cggcaatctc agcgagcaaa tcaccactgg cctgttcacg
    19501 acttaccgga gcggataatg tttccctgag ggcgttgcgt tcctccgcta actgctgctt
    19561 tttttgccgt cgctcttgtt caatctcctg tggataaaac gcttcacgca ggctttgcct
    19621 gacttgtaaa actgactcat ccatctgctg ctgcgcaagc cagatagccc cggtcagaag
    19681 aagactgccc gcaacaataa tcccgccttc cgtaacgagt cggttttgcc cagataaacg
    19741 cgcgctcagc caacccaacg cgccacccgc aatcaggccg ccaatgtggc aagcgttatc
    19801 tattcctgtt tgccgggcac cgtacaacag tgtcagcgcc accataccca acagaggaaa
    19861 tactcgccgc tggtttttat ggtatgtgcc cgtacctgaa gcaaactgtg ttgctatcgc
    19921 tgcaccagct attcccagaa tcgccccgga tgccccaacg ctgatgacta tctgctcact
    19981 ttcgcgtaac gcataacagg cgctgatgag cccaccaaag acgccggaga ataaccaggt
    20041 aatcaggagt cgccatttcc ccagaatctg ttccgccacg cctcccacga ccaggaatgc
    20101 aaacatattc atcagcagat gagaaacact actgtgcaga aagatactgc tcaataagcg
    20161 ccagggttca cccgacaaag taagaggcgc aacgtttgcg ccgagatgga taagtgcgga
    20221 ttgatcgacc tcttcataac tcatccattg tgcgagataa caaaatatcc caatacagag
    20281 tgcaaaaagg ataagggtta ttgcgggtaa ttgaacattt aacggcttaa ccgaagatgc
    20341 agacataagg tgtttcttcc ttgaaaagac aggtaaaaag catttttccc gtgtgcagtt
    20401 atcatcccgg caaacgtccc atgccgaaaa gataattctg tccgaaatat gactttttca
    20461 gcctaaaatt acaatcgtaa ttaattcgcc cctaagtatt ttttggcatg taagttgctt
    20521 tatttactgt gtaagcgatg aaatcagccg cgacgcaacc aggttgcacc accacaaatc
    20581 agcggtagca ccaaaaaggt gcggtttaag aattttgctt cggaatggtg cggcacatct
    20641 cgcaaaaaca aacaataaaa cagcaatatc tttacattta gccaaactaa atgttaccta
    20701 acaggcacaa cactgcacaa taaagttgca gacgataaca acacaaacac tcacaacggg
    20761 tatccatgcg ttcttaacgc agaagataaa ggagttggat atgcaattac gtaaacctgc
    20821 cacagcaatc ctcgccctgg cgctttccgc aggactggca caggcagatg acgccgcccc
    20881 ggcagcaggc agtacgctgg acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg
    20941 tgaatcttca gtacctttct cttattacga caatcagcaa aaagtggtgg gttactcgca
    21001 ggattactcc aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca
    21061 ggtaaaactg attccgatta cctcacaaaa ccgtattcca ctgctgcaaa acggcacttt
    21121 cgattttgaa tgtggttcta ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc
    21181 tgatactatt ttcgtggtcg gtacgcgcct gctgaccaaa aagggcggcg atatcaaaga
    21241 ttttgccgac ctgaaagaca aagccgtagt cgtcacttcc ggcactacct ctgaagtttt
    21301 gctcaacaaa ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca
    21361 cggtgactct ttccgcaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgatgc
    21421 cctgctggcc ggtgaacgtg cgaaagcgaa gaaaccagac aactgggaaa tcgtcggcaa
    21481 gccgcagtct caggaagcct acggttgcat gttgcgtaaa gacgagccgc agttcaaaaa
    21541 gctgatggat gacaccatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga
    21601 taaatggttc aaaaatccaa ttccgccgaa aaacctgaac atgaatttcg aactgtcaga
    21661 cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca ctgaactaat tacaagaacc
    21721 aggggcggaa attccagccc tctcgattgt tacgtagcac ggacagacta tacgcctgat
    21781 ggtcgttccc catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc
    21841 agttaacgct gctaccctct tttttctgga gtagatttat gtctatagac tggaactggg
    21901 gtattttttt acaacaagcc ccgttcggca acaccaccta tcttggttgg atctggagtg
    21961 gttttcaggt gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt
    22021 catttttcgg cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt
    22081 atgtcgaact gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tatctggtga
    22141 tcccggagct gttgccggag aaaatcggca tgtggtttaa ggccgagctg gatcccaata
    22201 ttcagttttt cctttcatcc atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg
    22261 aacaggtacg cgccgccatt cagtcgttgc cgcgtgggca aaaaaatgcc gcgctggcga
    22321 tgggcctgac gctaccgcag gcttatcgat acgtactgct gcctaatgct tatcgcgtta
    22381 tcgtcccgcc gatgacttcc gagatgatga acctggtgaa aaactccgcc atcgcctcga
    22441 ctattggtct ggtggatatg gcggcgcagg cgggtaaatt gcttgattac tcagcccacg
    22501 cctgggagtc gtttaccgct attacgctgg cgtatgtgtt gattaacgcg gttatcatgc
    22561 tagtaatgac gctggttgaa cgtaaagttc gcctgcctgg caacatgggg ggcaaataat
    22621 gtacgagttt gactggagtt ccattgtccc ttccctgcca tatctgctcg acgggctggt
    22681 gatcacgctg aaaatcaccg tcacggcagt cgtgattggt attttgtggg gcaccatgct
    22741 ggcggtgatg cgcttgtcca gctttgcgcc cgtagcgtgg tttgccaaag cctacgttaa
    22801 cgtattccgc tcgattcctt tagttatggt tttgctgtgg ttttacctga tcgtgccagg
    22861 ttttctgcaa aacgtgctgg gattatcacc aaaaaatgat atccgtttaa tttcggcgat
    22921 ggtggcgttt tcgatgtttg aagcggccta ctattcagag attatccgtg ctggtattca
    22981 aagtatttct cgtggtcagt cgagcgccgc gctggcgttg ggaatgactc actggcagtc
    23041 gatgaaactg attattctgc cgcaggcgtt ccgcgcgatg gtgccgcttc tgctcactca
    23101 gggcatcgta ctgttccagg atacctcact ggtgtatgtg ttatctctgg ccgatttctt
    23161 ccgtaccgcc tcaaccattg gtgagcgtga tggtacgcag gttgagatga tcctgtttgc
    23221 cggatttgtt tatttcgtta ttagtcttag cgcgtcgttg ttggtcagct acttgaaaag
    23281 aaggacagca taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg
    23341 ctgaccgact gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct
    23401 ggttccggca aatcaacgct gattaaaacc gtcaacggcc tcgaaccagt gcagcaaggt
    23461 gaaatcaccg tcgatggtat cgtggttaat gacaagaaaa ccgatctggc aaagctgcgt
    23521 tcccgcgtcg ggatggtatt ccagcatttc gagctgttcc ctcatctgtc gattatcgaa
    23581 aacctgaccc tggcgcaggt gaaagtgctt aaacgcgata aagcgcccgc tcgtgaaaaa
    23641 gcattgaaat tgctggagcg cgtgggactt tccgcacatg ccaataaatt tccggcgcag
    23701 ctttccggcg gtcagcagca gcgtgtggca atcgctcgcg cgttgtgtat ggatcctatt
    23761 gcgatgctgt tcgacgaacc gacatcggcg ctggatccgg agatgatcaa cgaagtgctg
    23821 gacgtgatgg tggaactggc aaacgaagga atgaccatga tggtggtgac ccacgaaatg
    23881 ggttttgccc gtaaagtggc gaatcgggtg atctttatgg acgagggtaa aattgtcgaa
    23941 gactcgccga aagacgcttt cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc
    24001 gcgaaaatcc tgcattaatc actctggcgc gcatacccaa ttgcgcgcca tctcacgctt
    24061 tatcgtttcg cccctcgtat ctgttacatc ctggcgttac ccttgtcgca aagaagcaca
    24121 caacaaggag caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgatgac
    24181 gctatcgcaa tagttctcgc cctcgcctca ccagagcttg atgtcaaagc aattacgtct
    24241 tccgccggaa accagacacc agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg
    24301 cttaatcgca ccgatattcc ggtagccggc ggcgcggtaa aaccgttaat gcgtgagttg
    24361 attatcgcgg ataatgtgca cggcgaaagc ggacttgacg gcccggcact accggaaccg
    24421 acgtttgcac cgcaaaagtg tacggcggtg gagctgatgg cgaaaacgct acgtgaaagt
    24481 gcggaacctg tcacgattgt gtctaccgga ccgcaaacta acgttgcctt gctgctcaat
    24541 agccatccgg aactgcatag caaaattgct cgtatcgtga ttatgggcgg cgcaatgggg
    24601 cttggtaact ggacgcctgc ggctgaattt aatatttacg ttgacccgga agcggcagaa
    24661 attgtcttcc agtcagggat cccggtggtg atggccggtc tggatgttac tcataaagca
    24721 caaatccacg ttgaagacac cgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt
    24781 gttgccgaac tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc
    24841 ggtgcaccgc tgcatgaccc atgcaccatc gcctggctgt tgaaacccga gttgtttacc
    24901 actgttgagc gctgggttgg cgtggaaaca caggggaaat atagccaggg tatgacggtt
    24961 gttgattatt attatctgac tggcaataaa ccgaatgcca ctgtaatggt cgatgttgat
    25021 cgtcagggct ttgttgattt actggctgat cgtctgaaat tttacgctta acacacgtca
    25081 ttgattaaga tcaccacgcg ttacctgggt ggtcttaatc tgtttatctc tttttctttc
    25141 aagatataaa tgcaatggat aatgcagaac tcgccattgg tatcgatctc ggcactacca
    25201 atagtttaat tgccgtctgg aaagacggtg ccgcgcaatt aattccaaat aagttcggtg
    25261 aatatttaac accatccata attagcatgg atgaaaataa tcatatttta gtcggaaaac
    25321 cggctgtatc acggcgtact tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa
    25381 tgggcagtaa taccaactgg cggttaggca gcgacacatt taacgcgcca gaactgtcct
    25441 ctttggtatt acgctcatta aaagaagatg ccgaagaatt tctgcaacgt ccgattaaag
    25501 atgtggtgat ctccgttccg gcttatttca gcgatgaaca acgcaagcat acccgtttag
    25561 cagcggagtt agccgggtta aatgcggtac gcttaattaa tgaacccaca gcagctgcga
    25621 tggcatatgg cctgcatacg caacaaaata cccgctcgct ggtttttgat ctcggtggcg
    25681 gcacgtttga cgtcacggtg ctcgaatacg ccacgccggt gattgaagtt cacgcctccg
    25741 ctggcgataa cttcctcggt ggcgaagatt ttacccatat gttggtcgat gacgttttaa
    25801 agcgcgcgga tgtcgccagg accacgctta acgagagtga actggcagcc ttgtacgcct
    25861 gtgtggaagc ggcaaaatgt agcaatcaat cgccattgca cattcgctgg cagtaccagg
    25921 atgaaacgcg ggagtgcgaa ttttacgaga acgaactgga agatttgtgg ttgccgctgc
    25981 tcaatcgctt gcgagtgccg attgaacagg cgttgcgcga tgcacgtctg aagccgagtc
    26041 aaatcgacag tctggtgctg gttggcggcg cgtcacaaat gccgctggtg cagcgaatcg
    26101 ccgtgcgtct gtttggcaaa ttaccgtatc aaagttacga cccgagcacc attgtcgcgc
    26161 tgggcgcagc aatccaggcc gcctgccgct tacgcagtga agatattgaa gaggtgatcc
    26221 tcactgatat ttgcccttac tcgttgggcg ttgaagttaa ccgccagggc gtttccggca
    26281 ttttctcgcc aattattgaa cgaaacacca cagtacccgt gtcgcgtgta gaaacttact
    26341 cgaccatgca cccggaacag gattcaatta cggttaacgt ctatcaggga gaaaaccaca
    26401 aagttaaaaa taacattctg gttgaatcct tcgatgtgcc gttgaagaaa accggggctt
    26461 atcagtcgat tgatattcgc tttagttatg atatcaacgg cttgcttgaa gttgacgtgc
    26521 ttctggaaga tggcagcgtt aagtccagag tcattaatca tagcccggta acattgagcg
    26581 cgcagcagat tgaagagagt cggacgcggt tatccgcatt gaaaatttat ccgcgcgata
    26641 tgctcatcaa ccgcaccttt aaggccaaac tggaagagtt gtgggcgcgg gcgctgggtg
    26701 acgagcgaga agagattggc cgggtgatca ccgattttga tgcggcgttg cagtcaaacg
    26761 atatggcccg cgtcgatgag gtcaggcggc gggcgagcgt ttatttagcc attgagacct
    26821 cataatcacg gctgccggat gcggcgtaaa tgctgcatcc ggcatcggcg caaaaaacac
    26881 atcgtcaact aaacaacatc gcgattttgc cgagagcata acctataacc agcaccgtta
    26941 aactgatttt gataatgctg gtaatgctct gtggtttttc accctcatca tttttttctt
    27001 catggttttc aggtgttggc tcatcgacat cttcatcctc cggctcgagt tttactttcg
    27061 tcaacagcat ccgggtgata atggcctcta atggatgagc atctccgcct tccagccttg
    27121 tattaagacg ggccagcatc tcttgtttcg gaaacgcttc aagtactacc tgttccgcta
    27181 cccgatacaa atccagtaac ggtgaatctt cttctgtctc gctaccgact ctcgatttcc
    27241 accacgccca tgctttgcgt ttgaagaata acgactcaat aaacgattga cctaacggcg
    27301 ataattttcc gctatgtaaa atttccgccc ataacagccg cgtccgggcg ctctcaccag
    27361 gaacatattt cagcggttga ccttgcgcat ctaccatgct ccgcgcttcg ataaccatca
    27421 ctaataacgc gaaataatcc gggcaatgac gcttgctcca ctgcaacaac aaattatcag
    27481 cttgtgtaga tgggtaactt tcccggtacg cgcagagatc ggcaagcagg ctttccccct
    27541 cgccgcagta caacctttga gcacacaagt aatatcgggc atcttcgctt tctggttctt
    27601 ccatttgcca ctgtttggct acaggaatga gttccgccat acctgtttgc accgagctgt
    27661 accagcgcaa cagtttgcga tgtaactttg catcatcagg aatgaaccac ggaccttcca
    27721 tcgccaaaaa cgcagtgacg tagtcaggat ggtaacggca gcaacgttcc agcgcgaagt
    27781 aatactcaac agtctggttc tgcaacgcaa cgggatattc cagaaggcta aggaaatcga
    27841 aacaatcacc agcctgaatg gcctcaagaa aagcctccct ttcttcctca tccattcctt
    27901 cgctgtcatt aaacgaatgc cagttcaaac gctctgccag caaagaagca caatttaatg
    27961 aaagatatcg cgcttctata gcgattgcac acagcggcca gcgtaattga tcgacatcct
    28021 ccatgttcca ggtatttaat tgctgaataa atttctgcca ggcggagggc tgaaaacgat
    28081 cactttccga atccagtaat gtcctgaatg cacgcagtat ttcatgttca gcggcggcat
    28141 ctttttcttc atcatctgct tcctcgacag gatttaccgc cagtcgcagc gcctcttcat
    28201 aggcctggcg taatgcttta aacccctgcg gatcggtttc gggatgacat aacggtaagc
    28261 gagcaagata agcctggcgg ataatgtcta tttgcgtcgt gctttcaatt tcgaggattt
    28321 gccaacatgt tttcataggt cgctcacatc cttaagcgtt accgggcatt caggctggac
    28381 agttaatggc tgccagggaa gcgtggcata gaaacgatcg cttcccgctg ccagctcttc
    28441 gaacatccga ctcccggcgc tatcgtattt gtacagggtt agcgcctcgc gtagcgaagt
    28501 gttgtcttcg cactgagaag attgccagta gagacgtcct aacgaatagg cggaaaaata
    28561 gtgcatccag ctgtgatagt aatggtgagc tcgtacatag acgcgcgatt gcagccataa
    28621 actctcttct tccgtcagcc agttgttgag cacaccgacc cgtgacagaa accccattcg
    28681 aacataatcc caggctttga ttcccccttc tccacagcac atagcggttt gggcaacgaa
    28741 ttgggtataa atcaaccctc tctccgagcc accagcaatt aatgctttcc actcttcagg
    28801 ggaagagcga tgccatgtca gatataagcc agctaaatca ttggcatgac catcatcagt
    28861 catctgataa atagtctgaa ttaaatcatc acgattattt attccccagg atgatgagag
    28921 atcgaattct tttataccgg tatacatttt tggcgagtca tgcgcgggtt tgcaaccctg
    28981 attaagtatt tccatgggcg cagaaagcgc aaaaagaaga tattgttctt ccttattcat
    29041 ttgttaat
//
LOCUS       DAZGAN020000053        28651 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGAN020000053 DAZGAN020000000
VERSION     DAZGAN020000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.053"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..242)
                     /locus_tag="ACHKYO_RS13045"
                     /old_locus_tag="pgaptmp_002609"
     CDS             complement(<1..242)
                     /locus_tag="ACHKYO_RS13045"
                     /old_locus_tag="pgaptmp_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4429436.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHKYO_RS13050"
                     /old_locus_tag="pgaptmp_002610"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHKYO_RS13050"
                     /old_locus_tag="pgaptmp_002610"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HGU4429437.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="ACHKYO_RS13055"
                     /old_locus_tag="pgaptmp_002611"
     CDS             2255..3904
                     /locus_tag="ACHKYO_RS13055"
                     /old_locus_tag="pgaptmp_002611"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU4429438.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGVYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3985..4557)
                     /locus_tag="ACHKYO_RS13060"
                     /old_locus_tag="pgaptmp_002612"
     CDS             complement(3985..4557)
                     /locus_tag="ACHKYO_RS13060"
                     /old_locus_tag="pgaptmp_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGU4429439.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRT"
     gene            5077..5976
                     /locus_tag="ACHKYO_RS13065"
                     /old_locus_tag="pgaptmp_002613"
     CDS             5077..5976
                     /locus_tag="ACHKYO_RS13065"
                     /old_locus_tag="pgaptmp_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4429440.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            6025..7038
                     /gene="yhjD"
                     /locus_tag="ACHKYO_RS13070"
                     /old_locus_tag="pgaptmp_002614"
     CDS             6025..7038
                     /gene="yhjD"
                     /locus_tag="ACHKYO_RS13070"
                     /old_locus_tag="pgaptmp_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HGU4429441.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7449..8771
                     /locus_tag="ACHKYO_RS13075"
                     /old_locus_tag="pgaptmp_002615"
     CDS             7449..8771
                     /locus_tag="ACHKYO_RS13075"
                     /old_locus_tag="pgaptmp_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4429442.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(9005..11065)
                     /locus_tag="ACHKYO_RS13080"
                     /old_locus_tag="pgaptmp_002616"
     CDS             complement(9005..11065)
                     /locus_tag="ACHKYO_RS13080"
                     /old_locus_tag="pgaptmp_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU4429443.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(11135..11902)
                     /gene="pdeH"
                     /locus_tag="ACHKYO_RS13085"
                     /old_locus_tag="pgaptmp_002617"
     CDS             complement(11135..11902)
                     /gene="pdeH"
                     /locus_tag="ACHKYO_RS13085"
                     /old_locus_tag="pgaptmp_002617"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HGU4429444.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            12134..13063
                     /gene="kdgK"
                     /locus_tag="ACHKYO_RS13090"
                     /old_locus_tag="pgaptmp_002618"
     CDS             12134..13063
                     /gene="kdgK"
                     /locus_tag="ACHKYO_RS13090"
                     /old_locus_tag="pgaptmp_002618"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HGU4429445.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(13159..14655)
                     /locus_tag="ACHKYO_RS13095"
                     /old_locus_tag="pgaptmp_002619"
     CDS             complement(13159..14655)
                     /locus_tag="ACHKYO_RS13095"
                     /old_locus_tag="pgaptmp_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU4429446.1"
                     /translation="MQGTKIQLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLADSTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(14876..16162)
                     /gene="dctA"
                     /locus_tag="ACHKYO_RS13100"
                     /old_locus_tag="pgaptmp_002620"
     CDS             complement(14876..16162)
                     /gene="dctA"
                     /locus_tag="ACHKYO_RS13100"
                     /old_locus_tag="pgaptmp_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HGU4429447.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(16345..18333)
                     /gene="hmsP"
                     /locus_tag="ACHKYO_RS13105"
                     /old_locus_tag="pgaptmp_002621"
     CDS             complement(16345..18333)
                     /gene="hmsP"
                     /locus_tag="ACHKYO_RS13105"
                     /old_locus_tag="pgaptmp_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HGU4429448.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18415..21888)
                     /gene="bcsC"
                     /locus_tag="ACHKYO_RS13110"
                     /old_locus_tag="pgaptmp_002622"
     CDS             complement(18415..21888)
                     /gene="bcsC"
                     /locus_tag="ACHKYO_RS13110"
                     /old_locus_tag="pgaptmp_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HGU4429449.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQHKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPANADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQRTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21870..22976)
                     /gene="bcsZ"
                     /locus_tag="ACHKYO_RS13115"
                     /old_locus_tag="pgaptmp_002623"
     CDS             complement(21870..22976)
                     /gene="bcsZ"
                     /locus_tag="ACHKYO_RS13115"
                     /old_locus_tag="pgaptmp_002623"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HGU4429450.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22983..25322)
                     /gene="bcsB"
                     /locus_tag="ACHKYO_RS13120"
                     /old_locus_tag="pgaptmp_002624"
     CDS             complement(22983..25322)
                     /gene="bcsB"
                     /locus_tag="ACHKYO_RS13120"
                     /old_locus_tag="pgaptmp_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HGU4429451.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(25333..27951)
                     /gene="bcsA"
                     /locus_tag="ACHKYO_RS13125"
                     /old_locus_tag="pgaptmp_002625"
     CDS             complement(25333..27951)
                     /gene="bcsA"
                     /locus_tag="ACHKYO_RS13125"
                     /old_locus_tag="pgaptmp_002625"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HGU4429452.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27948..28649)
                     /gene="bcsQ"
                     /locus_tag="ACHKYO_RS13130"
                     /old_locus_tag="pgaptmp_002626"
     CDS             complement(27948..28649)
                     /gene="bcsQ"
                     /locus_tag="ACHKYO_RS13130"
                     /old_locus_tag="pgaptmp_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HGU4429453.1"
                     /translation="MTAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWARA
                     MLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGRYQ
                     WILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRIGS
                     QVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANWC
                     LLNYSGLKTPVGSAS"
BASE COUNT         6757 a         7907 c         7237 g         6750 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtcacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccgcc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 taaacttcga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaggatt tcgacgccat ttcaatcggg ttcaacggcg gtccacctgc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gtagtgcggt ataacgcgtt ggtggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggtg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaagcctaa aaccttatta ttttcactga cagcagatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagggagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc cccgacagta aaacctttcc cgactgcgca cccaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctgc tggcgctacc gcagtcatat attgtcccgg gcggccgttt
     2761 tagcgaaacc tactattggg attcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttattggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggagcggcc tcaggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg cgtctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaatggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     3961 ggagatattt aacatcattg ccgcctaggt gcgatttttc acttccagac ggcgatacag
     4021 ggattccaga tgcgctttta ccgttccggt actgatattc agcgctctgc cgatctcctt
     4081 atttgattcg cccgccgcta acatggttaa aatctcccgc tggcgggcgc ttaacgattt
     4141 gagatcttta atgtcctttt ccggcgtcgt ccgccagtct ccaggcagaa acatcatccc
     4201 catcgccgca ctatttaccg ccaacgcaaa tgtctcgacg gttgaatcac gaggcacaat
     4261 ggccagcaca ttaaaatgga taacttcctg taaccaccgt ttattgcaat ccgtcgccgt
     4321 gattaacacc ttaacctcag gaaactgcac cacggttttt tgcagcaacc agtagcaaaa
     4381 ctcaccatcc tgatcgccat cgagcataac taaggcttca gggtaacttt ccagcttttg
     4441 ccataactcg tctgcctgac tggccccctg aatactcact cctggaatac gctgctgtaa
     4501 actgattttc attccatgaa taaatattga ctgcctgtca aacatgacta tttgcataac
     4561 tgaatctcca cctgaatacg ttaaaaagac ttaagtagtg gaagggtatt acccgcgaga
     4621 aaaaataaga attcgccatt tggcggtggc cattctacag agatgacgcg tagaaaatag
     4681 ttaccgatat aaatagttac agctaaacgc ctgaaattac atgtcgaggg cactatttaa
     4741 aacaattttg aggatttcct tatattggtg gttagtacgc atgcaattaa aaatgaaact
     4801 ccgcgaccac aagcaaaaat aacaaacggc aatgagacaa aaataagcac aaatagccaa
     4861 cacgtcctct attcacttta aagggaatcg ctgaaaaata cgctctgttt agggggattc
     4921 acctttctca gaaagctatt ccgccctttt cctgctgaga aatcgccaca ttcgtcatga
     4981 caacattgtg aaacccggca ttagatgtta gaaaaaacaa taaacaatgc gatatgcgcg
     5041 ttactgtttc gctgtgaaca acaatcggtc aaagaaatgg ataaaattca cgcaatgcag
     5101 ttgttcatca aagtcgcgga gctggaaagt ttttcccgcg cggcggattt ctttgctttg
     5161 ccaaaaggaa gtgtttcgcg ccagatacag gcactggaac atcaacttgg cacccagctt
     5221 ctccagcgca ccacgcgacg ggtcaaactc acgccagaag gcatgaccta ttatcaacga
     5281 gcaaaagatg tgttgagtaa tctcagcgaa ctggacggtc tgtttcaaca ggatgccacc
     5341 agtatcagcg gtaaattacg catcgacatc ccgccaggaa tcgcgaaaag cctgttactg
     5401 ccgcgcctgt cggaatttct ctatctgcat ccgggaattg agctggaact gagtagccat
     5461 gaccgtccgg tagatattct tcatgatggt tttgattgcg tgatacgcac tggcgcgtta
     5521 ccggaagatg gcgttatcgc ccgtcccctc ggcaaactga ccatggtcaa ctgtgccagt
     5581 ccgcactatc tgacgcgctt tggttatccg caaagccccg atgatctgac ttcacacgca
     5641 atagtgcgtt atacaccgca cctgggtgta catccgttag gttttgaggt tgccagcgtt
     5701 aatggcgtcc agtggtttaa gtctggcggc atgttgacgg taaacagtag cgaaaactat
     5761 ctcgccgccg gtcttgccgg tctggggatt attcagatcc cgcgcattgc cgtgcgcgaa
     5821 gccctgcgtg ccgggcggct tattgaagtg ttgccaggct accgtgccga gccgctctcc
     5881 ctttcgctgg tttacccgca gcgtcgggag ctttcccggc gtgtaaacct gtttatgcag
     5941 tggctggctg gcgtaatgaa agagcacctg gactgaccga ctatactttt taagaatgac
     6001 cacgacagaa ggacaaaaga gcggatgacg caggaaaacg agatcaaacg tcccacccag
     6061 gatctggagc acgagccgat taaacagttg gataatagcg agaaaggcgg caaagttagc
     6121 caggcgctgg aaaccgtcac caccactgcc gaaaaagtcc aacgccaacc ggttattgcg
     6181 cacctgattc gtgcgacaga acgctttaac gatcggctgg gtaaccagtt tggtgcggct
     6241 atcacctatt tctcattttt gtcgatgatc ccgattttga tggtatcgtt tgccgcaggg
     6301 ggctttgtgc tggcctccca tccgatgttg ctacaggata tcttcgacaa aattctgcaa
     6361 aacatcagcg atccgacgct agccgccacg ttgaaaaaca ccatcaacac cgccgttcag
     6421 cagcgtacga ctgtagggct tgtcggcctg gcggtggcac tttattccgg catcaactgg
     6481 atgggtaacc tgcgtgaagc gattcgtgcc cagtcgcgcg atgtctggga acgctcgccg
     6541 caagatcagg agaagttctg ggtaaaatat ctgcgtgatt ttatttcact gattggtttg
     6601 ttaattgcgc tgattgtgac gctgtcgatc acctcggttg ccggttcggc gcagcaaatg
     6661 attattagcg ccctgcacct gaacagcatt gagtggctga aaccgacgtg gcggttgatt
     6721 ggcctggcga tttccatctt cgccaactat ctgcttttct tctggatctt ctggcggttg
     6781 ccgcgccacc gtccgcgcaa aaaagccctg attcgcggaa catttctggc tgctattggt
     6841 tttgaagtga ttaaaatcgt gatgacctac accttgccat cgctgatgaa atccccctct
     6901 ggcgcagcat ttggttccgt gctggggctg atggcatttt tctacttctt cgcccgtttg
     6961 acgctgtttt gcgcggcgtg gattgccacc gccgaatata aagacgaccc gcgaatgcca
     7021 gggaaaacgc agccttaaaa taggccggat gcggcgtaaa cgccttatcc ggcatacgat
     7081 tttattcggc ctggctcccc gtaggccgga taagatgcgc cagcatcgca tccggctata
     7141 atgcgcacat aacctcttga aactcttccc cagagcctct tcagccatct atttgggagc
     7201 aaacaatttc attccaactc ataaccccag catataaatc cagttggtaa cttttattta
     7261 acctgaaacc agttttatcc actatttata aaattatgtg aagcatttca tagaagaaaa
     7321 atcactggcc taaacattat cccctttttg cctggttttt gaccatttcc gcgatttgtt
     7381 acacattgaa atatcacttt tgctgtgcgt aatatggcta ttcgttagcc aaaaaataag
     7441 aaaagattat gcaagcaaca gccacaacac tcgaccacga gcaagaatac acgccgatca
     7501 actcgcgtaa taaagtcctt gtcgcctctc tcattggcac ggccattgag ttcttcgact
     7561 tttacattta cgccactgcg gccgttattg tgtttccgca tatcttcttc ccgcagggcg
     7621 atccaacggc agcaacgcta cagtcgctcg ccaccttcgc catcgccttc gtcgcgcgcc
     7681 ccattggctc tgccgttttt ggtcattttg gcgatcgcgt tgggcgtaaa gcgacgctgg
     7741 tcgcctcgtt gctaacgatg gggatttcga ccgtggtgat tggtctgctg ccgggctatg
     7801 ccacgattgg tattttcgcc ccgctgctgc tggcgctggc tcgatttggt cagggtctgg
     7861 gcttaggcgg tgaatggggc ggcgcggcgc tgctggcgac tgaaaacgcc ccaccgcgca
     7921 aacgtgcact gtatggctcc ttcccacagc tgggcgcacc gattggcttc ttctttgcca
     7981 acggcacttt cttgctgctt tcctggctac tgaccgacga gcagtttatg agctggggct
     8041 ggcgtgtgcc atttatcttc tcggcggtgc tggtcattat cggcctgtat gttcgcgtgt
     8101 cgctgcatga gtcgccggtg tttgagaaag tcgctaaagc gaaaaaacag gtgaagatcc
     8161 cgctgggtac gctgctgacc aaacatgttc gcgtaaccgt actgggtacg ttcattatgc
     8221 tggcaaccta tacgctgttt tacatcatga cggtctactc tatgaccttc agtaccgccg
     8281 ccgcgccagt tgggcttggc ctgccgcgta acgaagtgtt gtggatgctg atgatggcag
     8341 ttattggttt tggcgtgatg gtgccagtcg ccggattact ggctgatgcc tttggtcgcc
     8401 gtaaaagcat ggtaatcatc accacgctga tcatcctgtt cgcgctgttc gcctttaacc
     8461 cgctgctcgg ttctggcaac ccgattctgg tttttgcctt cctgctgctg gggttaagtc
     8521 tgatgggact gaccttcggg ccgatgggcg cgctgttacc agagctgttc ccgacagaag
     8581 tgcgttacac cggagcatcg ttctcttaca acgtagcgtc gattctcggg gcttccgttg
     8641 caccatatat cgcagcctgg ttgcaggcta actacgggtt aggtgcggtg gggttatatc
     8701 tggcggcgat ggctggcttg acgttaatcg ccctgctgct gacccatgag acgcgacatc
     8761 agtcgttgta agtatcgtgt gggcagccac cctcacccca gccctctccc tgaaagggag
     8821 agggagaaaa gcggagcgag atgttatatc gcgaacataa cagccctccc ccagaaagtg
     8881 aacggaaaag cggtactcgt tgtttcaaca cgcacaaaac agtcccctcg cccctttggg
     8941 gagagggtta gggtgagggg aacacgctgc tcccgaactc accagcaaac acaatccaac
     9001 ctcatcactt cttcatctgc gacaatatcg tccgacactg attagcctcc ccttcggaag
     9061 gtgagatcag tgccagtaac gccgccgccg gtgtaaccag cgttgccagt gccgcagcaa
     9121 cagcaccacg ggcaatcaac ggcccggctt tcaccccagc ctgcggattt ttaaacgtcc
     9181 cacgcacata cagcggcgaa cgcagtgtga taatccgaat tcctttactc tccggatcaa
     9241 tagtcaaatc cagctgttcg gaagcaaaac ttgccgtgcc ggtaacgtta atcaacgcgt
     9301 tctcagtatc gaaagcaaag atctgcgggc gcgccacgcc gttggcaata tccagattcg
     9361 ccgccgcgca gttcacccgc acctcatcat caccaaatat cgcaccgaca atgtagttgc
     9421 cgacattcag cccaacaatc tccatcaggt tgcggctcac cagcccgtca ttcatcaaca
     9481 gtttcaggtt gccgttactg ttgcctaaaa gtgccgccac cgagttaccg ctaccgcgca
     9541 gttccgcgtc accgttcatt tcccccagcg tcttctgcat cagttccaca tcgggcatca
     9601 gttctttcag tttcagtcga cgagcctgaa tatctgcccg cccctgcatc ggctttttat
     9661 cgccttccag atgaatattc gccgcaatgc tgccacccgc catgccaaat ttcagcggtt
     9721 gcaggcgcag gtcagcattt ttgaggatga tatgagtaga aagatcgcta atcggcaggc
     9781 tactgccatg ctcaatgcgc cgccctttga agcgaacatc ggcatccata acgtcccatt
     9841 tatcggtttc gaagcggtca taaggcagca ctttgcccgc aggctgaacg cttttttcgc
     9901 ccttcttctg ttcagaccgt ttcgactttt cagccccttt cccggaatca acgccaatca
     9961 acggtcccag gtccgccagc cgcaattgcc gcgactcgac atcaccttcc agttttggtc
    10021 gtggcttgcc ggtggtgtag atcagagaac cgtggatatc gctatcgcca atgcgcccat
    10081 taaaaccgcg ataatcaaag accgacgatt tttcagtgtc gattttcgct accagccgac
    10141 catctgtttc aaacggcggg gtatcgggca gcagaacgcc cgtcagttca tagagatcac
    10201 ccagtgaatc gccagaaaat ttaagccgta aatcgacacc gcccatcttc attgggtcat
    10261 tcacgacgcc atcaaaagca acacgggtat tacctgaacg gaaatcagcc tgtaccggaa
    10321 acggcgtccc ttcgccacgc agcgccagca taccgcctat ttttcccgta ccagtgagcg
    10381 gttcaccgtt ataacgtccc tgcgccttca ggccaaaaac gtaatcgccc accttttctt
    10441 tatccgcttt acctttcgat ccagtaactt cgctgaacgg cagcggctta cctaacggat
    10501 cgacaaaaat ttccagatcc gctttgctca ctttgtcatc aatggcgatc cgcccttgat
    10561 cgaaaagaat attatccagc cgaaacgacc atgccgacgg ctttgcattc gcgtctttgt
    10621 tatcatcgtt ggcgagatta aacgtccagt tattgttctt ttcagagagg cgaatcaggc
    10681 gcgcgtcggg cttttcgagc ttgatccacg gcagccagac cgttttggtc agcagcgcca
    10741 gcggggccag cgttgcctct acgcgtggca aatgcaccat cgtgacttcg ggaatatccg
    10801 gtggattgcc aagaatgatg tcttccgcgt gtacatgggg ccatggcacc cagctgcgcc
    10861 agccagtttc ttgtttttgc cgctcccaca ccacgcccag atcgccacgg atagcgaacg
    10921 gacgattcaa ctccgcagag actttctggt tgatggtcgg tttgagtcga ttccagtcaa
    10981 aagttgcaat caaaatgatc gccacgacaa tcaacaacaa gaaagcccct gaaatcgcag
    11041 cggttatttt gcctgccttg ctcatcgttc gcttcctcat attcttcctg tgccagtcct
    11101 aaagatagtc cagccaggcg gaaaatgagg cagcttatag cgccagaacc gccgtattca
    11161 gcgtttctat cggtgccggg cgtgaaagaa accagccttg tgcggcgaat gcgggcgagt
    11221 tctgaacatc acgccactct tccggtgttt ctacgccctc gacaatcacc ccgcgacaat
    11281 agcgattcat tagatgtaaa agctgagaaa agagtgtgcg gccttccggc gactgacgca
    11341 gcatcacaaa cagttctcgc gcgattttga tgtagtcata acgcacttca cttagtgcag
    11401 agaaatttgc catcccggta ccaaaatcat ccagccacag cgggccaaat tcacacatcg
    11461 aggcaaaggt tgaatctttc ggcagacgga tatgctccac cagttcgaaa cgcagccagg
    11521 gaagacgctc aatctggcgc aggatttttg gttgctgacg cagggcgatg agcgtagggc
    11581 catcaatatt gaccgatgcc agcaggccgt gctctataaa gaagtcggct ttttgcgcca
    11641 gcaaatcaat ctgctctttc acaacctcca tccgatggct gacggtgatt tcagtaaaat
    11701 agcgatccgg cggcaggcgt tgcgaagggt tcaagggatg cgtgaccacc gttaatagct
    11761 ccacggccat taaccgcccg catgtttgat agatcggctg ccaggtgtaa gcacgctcac
    11821 actgcaacca aaaacgccgt tcctgcaagc tctcgatgct tgcttcaggg ttgcttattc
    11881 gctggataac ctgccttatc atcaaagatg tcctgtgttt aaaagtgatg ctgcccggac
    11941 tcgacaaaga ttatcggcgc gcaagggcag aactttaccg cctggccggg gtaaatttcc
    12001 cctctccaca acggaatctt tcaggaatac gttctggctc aaaaaaataa tggaacactg
    12061 ttttaatatg gttgaccagc aaaccaccac agcgcaaact aacgctaatt ttttacagat
    12121 caggttcacg actatgtcca aaaagattgc cgtgattggc gaatgcatga ttgagctttc
    12181 cgagaaaggc gcggacgtta agcgcggttt cggcggcgat acactgaata cttccgtcta
    12241 tatcgcccgt caggtcgatc ctgcggcatt aaccgttcat tacgtaacgg cgctgggaac
    12301 ggacagtttt agccagcaga tgctggacgc ctggcacggc gagaacgttg atacttccct
    12361 gacccaacgg atggaaaacc gtcttccggg cctttactac attgaaaccg acagcaccgg
    12421 cgaacgtacg ttctactact ggcgtaacga agccgccgcc aaattctggc tggagagcga
    12481 gcagtctgcg gcgatttgcg aagagctggc gaatttcgat tatctctacc tgagcgggat
    12541 tagcctggcg atcttaagcc cgaccagccg cgaaaagctg ctttccctgc tgcgcgaatg
    12601 ccgcgccaac ggcggaaaag tgattttcga caataactat cgtccgcgcc tgtgggccag
    12661 caaagaagag acacagcagg tgtaccaaca aatgctggaa tgcacggata tcgccttcct
    12721 gacgctggac gatgaagacg cgctgtgggg tcaacagccg gtggaagacg tcattgcgcg
    12781 cacccataac gcgggcgtga aagaagtggt ggtgaaacgc ggggcggatt cttgcctggt
    12841 gtccattgct ggcgaagggt tagtggatgt tccggcggtg aaactgccga aagaaaaagt
    12901 gatcgatacc accgcagctg gcgactcttt cagtgccggt tatctggcgg tacgtttgac
    12961 gggcggcagt gcggaaaacg cagcgaaacg cgggcacctg accgcaagta ccgttattca
    13021 gtatcgcggc gcgattatcc cgcgtgaggc gatgccagcg taaacgcagc atatgcaaaa
    13081 ggctggataa agtgttaccc ctctatccag ctcatccata aatacctgat ggttgtgcta
    13141 actcatcacc atcaggcatt actgtgcagg tggaatatcc gtcacttcag gatgtacgtc
    13201 atccgtggcg acagaggtgg cggcagcagc agtcgatggg gccatgattt gatcccaggc
    13261 cgcctgcaac gctttcatgt taaattccgg ctcgcctttc ggctgcagca gtattaacgc
    13321 catatcattc gacaactgct gacgcagatc ctgatttaac atctccaccg tcaggctgtt
    13381 aaggaaatcc tgccgcagtt tctgatactg ctccggcgcg atatcgacga cctgattttg
    13441 caacgaacgc atccgctgac ccatcagaat atcggtatca gcccgcgcat aggcggcaaa
    13501 cagtttctgc aactccagtt tcttttgcgc cactaacgca ttgaactctt cttccggcag
    13561 acctttatcg cgaaccttcg ccagttcacg cgccaccaga ttaaggttgc tgttcagctt
    13621 gtcgtttggt gattcgatgt tgatggcaca ctgcgcacgc agatacagca cacggcagtc
    13681 aaatccaaga ccgatgtctt tgctgttact ggcgcttaac gcttgctgaa catgccagaa
    13741 cagcgcctcg cgggccaggt ccgcacgcca gtagcgcagc agtgcggctg attcacgaat
    13801 cggctgccac ggcgtatccc acatgataga taaccggtcc tgacgcaccg cgtcagtcat
    13861 aatactcacc gcttccgcac gcagcggaga aagcgtcggc accggagctg gcgtttcacg
    13921 tttgcctttc agttcgccaa acgttttgtt tatttggtcg acaaccgagc gcgcatccac
    13981 gtttcccacc accagtagcg tcattgcatc cggggtgtac catttctgat agaaatcttt
    14041 aattttttcc gcttcaacgg gttgtttcag cggatcggca ggatcgtgac ctaacaaggt
    14101 tgaccctttc agacgatagc gccaccagcc ctctttagta tcggcaggcc aggttgccac
    14161 catgtcctga ctttgcagcg cgtggttgat ggtttctggt gtgatggtca atttgccagt
    14221 ggcatttgcc agataagaga gcgcttcttt cagcaaatcg ttacggttat tgggcaaact
    14281 cagattaaac agcgtggtgt cataagagac aattaccggc ggcatcgggc gtttagggtc
    14341 gatcccctgc tgccacaatg aacgcgcctg tgctgcgtca aggccaccgc tttgcgttag
    14401 cgcaatacga gggatggcgt gactgtaacc gctctgttgt gtactatcgg cgagcgaacc
    14461 ggtattaacc agcaggcgaa tttcaacacg atcgctggga cgctgggggg tggtcagcac
    14521 ttgccactgt aaaccgttgg aaagcgtccc ctgttgccat gctggatcag gctggagcgc
    14581 atctgcctgc acatagccag cagtggccat catcagcaaa ccgcccgcta aaagttgaat
    14641 ttttgtgccc tgcatgtgaa cccctgatca actatcctgg taataaaaaa actgcccgtt
    14701 gtcggtcagt ctaaatgacg ttaaaaacac ttcgtgttag accgcaagaa catgaaaatg
    14761 tcacggaaga agtgaaataa acctgaactc acccaggtgg taagttcagg cacaggggtc
    14821 aattatgcgc aaacacccgc actcggggaa gggagtgcgg gcataagtga tgagattaag
    14881 aggataattc gtgcgttttg ccatccggcg cacgattatt cagcacatcg tccagttttt
    14941 tgtggtccag ttctttcacc cacttagcaa cgacaatggt cgctacgccg ttaccgacca
    15001 ggttagtcag cgcacgagct tctgacataa agcggtcgat accgaggatc agcgccagac
    15061 ccgctaccgg caaatggccc accgcagaga gcgtcgccgc cagcacgata aagccactac
    15121 ccgttacccc tgccgcccct ttagaagaaa gcagcaacac gattaacagc gtgatttggt
    15181 ggacgatatc catctgactg ttagtggcct gggcgataaa caccgccgcc attgtcaggt
    15241 atatcgatgt gccatcaagg ttaaacgagt agcctgtcgg gatgaccagc cccaccaccg
    15301 atttgcggca gccgagtttc tccatcttgt cgagcatacg cggcagcgcc gactcggaag
    15361 atgaagtccc cagtacaatc agcagttctt cacggatgta gcggataaat ttgaagatac
    15421 tgaaaccagt cgctttagcg attgaaccca ataccagcac cacaaacagg atacaggtaa
    15481 tgtagaaaca gataatcagc tgccccagtt gcaccagtgt gccgacgccg tatttaccga
    15541 tggtaaacgc cattgccccg aacgcaccaa taggcgccag acgcatgatc atattgatga
    15601 tgccgaagat gacctgcgag aaactttcga tgacgttaaa aatcagttgg cctttgctgc
    15661 ccagacggtg gagcgcaaaa ccaaacagta cggcaaacag cagcacctgc agaatgttac
    15721 cgctggcaaa tgcgccaatg acgctcgccg ggatgacatc cataatgaag gcgacaatgc
    15781 cctggtcttt cgcctgatcg gcgtaaaccg ctaccgcttt cgcatcaagc gttgccggat
    15841 cgacgttcat tccggcacca ggctgcacga cgttaacgat gataagacca ataatcagcg
    15901 cgatggtact gacaatttca aagtaaagca gtgcgactgc gccggtacga ccgaccgcct
    15961 tcatgctttc catgcccgca atgcccgtta cgacggtaca aaagatgaca ggagcgatga
    16021 tcatcttaat gagcttaacg aagccgtcgc caagcggttt catttgctcg cctatttcag
    16081 gatagaaatg gccaaggaga ataccaatgg ctatcgctgt caggacctga aagtaaaggc
    16141 ttttaaacag agaggttttc atagggtgtc ctttagtaaa accacaggtc ttgtaaggtt
    16201 atggggtacc tgcggcctta aaataacacc cagacaacat cacagaaatg tacctggatc
    16261 ataattgaaa caaaaaggtt aaaaagtttg agctggctcg cacaaaccag cactttttaa
    16321 agttttgtaa tcagtttggg gtagctactt ttcttccagg taactctctt cgaagatttc
    16381 aatagggagt gggcgagcaa aaaggaagcc ctgggcaata ccaacgcccg cttttgccag
    16441 ccagtcgcgt tgtgcttcag tctccacgcc ttcggcaatc atttgtaagt tcaggctctg
    16501 cgccagcatg ataattgcag caatcatgct gctatcttcc ggcaagcctt caacaaacat
    16561 tttgtcgatt ttcagtacgt cgattggcaa cgatttcata tgctgcagct gacgcagccc
    16621 tgcgtagccc atgccgaaat catccagcgc cacccgaact ccggcattgc gcagcggacg
    16681 gaggattgcc accgcagcat gagggtcgtc aatacgtcgg ctttctgtca cttccagaat
    16741 cagtgttccc ggctgaatgc gatagcgggt taacagttcc agcatatccg ccaccatatt
    16801 tgggtgcatc agttgcagcg cagagaggtt taccgacaag ggcagcataa tgccgcgctc
    16861 ttgccaggct gcaagcaatc gacaggactc ttccagcacc cagtgaccga cggtaaccat
    16921 cagcccacag cactcaatgc gatcgattaa gccatccggc aggtcccaac tgccatccgg
    16981 ttgctggata cgcagtaaca cttccgcact gaccagtttt ccgctggtca tctcgacctg
    17041 tggctgtaac caaatagcaa actgatgatt ttccagtgca ttaaggatat cgctctcttc
    17101 cgtcaaccgc ttctgggcgg cttccatctg ctgcggatca aagaactgaa tctgattctt
    17161 gcctttatgg cgagcggtaa atgccgcaga aatagcgcga ctgtaaagct gttcggcggt
    17221 gagatcgccg tagaacatcg ccacgccaat gctacagtgc ggacggagtt gaatacgttc
    17281 aatcggcagg cgctcgctca tgatagtgag cacttgctga cctaaggtga ttgcgtgcca
    17341 cggttcctgt acaccgttgg caatgacagc aaagtcataa ccgctaatct gcgcgaggat
    17401 catacgtggc gacagtaccg atttgagttt ttccaccagc gtcagcagca gaatttctcg
    17461 ttgcgcctct ttcagcacgc ccgcagtatc acgcagggtt tcacaggtga tgatcatcag
    17521 cgcggtggtt tgtttacgcg cgacaacctg ctccagcatc tccatcagca aggctttgtt
    17581 cggcaaatcc gacaccggga agcgcatcgc attttcgttc tgttcttcat aatggcgctg
    17641 cagcaattgc tggttgaggt tgtaactgcg caccaacata ccgatttcat cgtcctgatg
    17701 cagacgcggt aatgccagtt ggtgaccaac aagctcctgg gctgggatgg cgttaagttc
    17761 gcgagcaata ttgcgtaacg gatgcaaaat caggcggtta atgcaccagc tgatggcgac
    17821 ggtcaggata agcgacaaaa gtaagtaaat ggtcactaac gttgagaggg tgctcatcac
    17881 gaacttatac atacggaagg aatccgcctg tagtaccaga taggcaattg gctgcgggtt
    17941 tgccggacgt tcgagcgagt aaacgcccag cgagatttga accggtagct caaacaggcg
    18001 agtaaccatt accggcaccg ggcgctctgg aataaaactt ttacgcagcg cctggaactg
    18061 gttaggcagc actacatcgg cacggctgac cacgcctgcc ggtttaatgc tggcaagaat
    18121 agcttccgct tcgggaatat cgcctttcaa aatagctgaa gataagggtt cgcggacaga
    18181 gcgagcaatg ctttccagtt gcgtagccgt gttatagcga ttctgttgga ccagatggaa
    18241 cagcaaaacg gtgcaaaaaa taaaaacgaa caccaggaca acggctgcca ccattgccat
    18301 ctgcttgatt gttaacgagc gacttacgcg caaattgtct ccccccggaa atcccagatt
    18361 gccgcccgag tatacccgat cgcagcggca ttaagagagg cgctatctga aaacttacca
    18421 gtcggcgtaa ggtatcagcg gctgcggcgg taaatccatg tcaccctgcc atccggcggc
    18481 ggaataacgt acgtagagca ggaaatggct gggtgcgtaa tctttcgcct gctggatatc
    18541 aattgccgtg ccaacaaacc agttggaagt aacacgtcgt tcaagtaatg cccgcgccgt
    18601 gtagccgaag ccctgactgc tgccgccatc gttggattgc cgcgcagctt cttcctgcca
    18661 gtcggtcggg atcagattca tcagcggata acgcggcatg gttttggtgc gtgaatgcga
    18721 ccacgagcca gacgccccca gctcccacga ccagttttcc gtgcgctccc gccacatcac
    18781 cggtatggca aacgacaggt attcctgcgg actgtagtaa ccgccctgac cgagtgagta
    18841 gccactcaga tctttgtcgt aatgccagat catgttgttc aggccgattg tgacgcggcg
    18901 attgttctgg ttaatgacct tatagtaata gcccgtcatc cagcgcacgc gccagttatc
    18961 ttcgacattt ttaccggtta actggtcgcc actaagcgat gcccagacgc cgtttgcttc
    19021 acctttatcg tagctcagac ttagccccac accgtcggca cgtacgccac cccatttttt
    19081 cccggtattg ctcggggagt ctttttgccc accaaaggcc agcaaagaac tggagatggg
    19141 ccgacggtgg gcgttaacgg tgtaacccag cggcccgata tcatcgctgt aactgatgcc
    19201 gccgaccaca tccaccacgt tgaagcccat cggcgtggta ccgatatccc agctccagac
    19261 gtcatttcgc cagccgaccg ccacgctggc accggaatcc gactggctgc ggttgccgct
    19321 acagtcctgt aatgtacagg tgccccagtt gtcatcccat ttgccatcgg cattagtgga
    19381 gaaactgccg acgttcatat tgacgaaatc actgcgaaag aacatccgcc cgtcagaata
    19441 cggcgcatcc acctgcaaca tggtagtgtg cgctttcaga tcggagtaac caccggtgcc
    19501 gctcgaaccc cagtaatcgt gctcaagggt gacgttaaga tcctgctggc gatagaggtc
    19561 cgccgcatcg ctgcgcacgc cgcgtttcag ccagtcatct ttctcgtcgt tacgggtcag
    19621 tcgggtgaat gtgtcgttat cctgcggacg cgtcgtagtc acaccggatg cgaccatggc
    19681 gtctttgtag gtttccagcg cctgctttgg atcgcccgcc tgcgcttcaa acttcgcacc
    19741 gtcgcgcagc accatcgcgc tttccatcga cggtggctga gattttgcct gcgggatcaa
    19801 cttattaaac gtccgctgcg ctgctgcggt atcgccaagc tgcgcctgtg ccagcgccac
    19861 gcgccgctgt gtgttcagcg aggcgttatc ggtagcgggc agtttcgcca gctggctacg
    19921 tgccgccgct ttgtcaccgg cagcaatatc cacttccgtc agaccaagaa tggcgtcggc
    19981 gttagctggc tcccgcgtca ggacattctg atatgcagca cgggcggcgg tgtaatcacg
    20041 tcgttgttgc gcccagtcag ccagcgtgag gtcaatacgc gtggaaggtg gttgctgacg
    20101 cagcatcgct tccgcttcag cttctttgcc gctttctcgc aggcgattag cggtttccag
    20161 cacctgatcg ctttgcagtc gattaaccag ctcctgaata ttgctgttcc actgcgcgcg
    20221 cggcaggctg ttgatatgcg ccagcgccgc tctgtcctgg tcatgaccag agaggtacag
    20281 cccgtaagcg taaacctgct ccgggtcgtt cggcttatgc tgcgccagat tgcgcattaa
    20341 cgtatcggcc tggctgcgtt gtccggcctg ccagagatcc tgcgaaagtc ggtaagtaat
    20401 ccatacgctg ccggggtcca gcgccagtcg ttgccgctga agtgctgccg cctgcgccca
    20461 tttgccctgg ttttccagtg cctctgcctg ctgtgccaga cggtcgtttt gcaggctgcg
    20521 ttcgatatca tcaatgctac gccgctgact ggcagagagc gaggcgataa acgcttcagc
    20581 tttttctggc gattgctggc ggtaaatatt tgccagcccg cgcacggcgt tagtgttgcc
    20641 gctgtccata cgcaaggtct gctgataata acgttcggcg gcgggataat cttttcgcgc
    20701 catcgccaca tcgcccagcc ccagcactgc ataactgtcg gtgttatcga cattacgcgc
    20761 ctgctggaac aggcgttctg cccggtcagg attattggct ttcagcgcag catcgccctg
    20821 ctggatcgcc agccagtagc ggtttacttt cagcagactg ttccatttgt cgttgttgct
    20881 gctgtgcggg tccagtgcga gggctttttc cagattcgcc actgcattgg cgcgatcgcc
    20941 tttctgagaa tacgcctggc ccagcgcccc cagagcttca ctgtctttcg ggttcgcccg
    21001 caccgcctgt tgtagttcgg gaatggcttt acccgccata ccagagtcca ccgccgctaa
    21061 accttgcgca cgagcgcgga aagcaggatc ggccagctgt ttttgctgtt ctgccagttg
    21121 cgattgcgca gccgccacgc tatcgccatc actaaagatc gagagatatt ttttcagcgc
    21181 cgacacactg gcatcactga cgggcatgtc tttaatctgc ccgtaccaga ttttagaggc
    21241 cccttcgcgc ccggcgttcg attttgccat ctgttccagg acggcaaaac cttcgtcacg
    21301 gcgatcgcta ctaaacagca atagcgccag attgttttgc aggcccgtat tgcccggtgc
    21361 atccgcattg atgcgtttta actgattaat cgcttcgcca cggcgagccg gaattttcgc
    21421 caccgtactc cagtactcga cggcaatgtc accttccggc ggcgcaccgt tgaacagttt
    21481 gttgtaactc gccacagctt cttctgcatg accggtcgtc gcctgcaatc gtgcctgttg
    21541 cagtgcctga cgaccatccg gcgtggaaag tagcatcgta gtccgcgacg atttatacgc
    21601 atttgaactc ggcgctaact gcgacagccg atcgagctgt ttttgcgcgc catcaatatc
    21661 gccctgacgt aacaaagaac ggaaacgggc ggcaacgacg tccgggttat tcggatcaat
    21721 aagttccagc cgatataacg actgttgcac cagatcttca cgatgggtcg cttcgcctaa
    21781 ccgaacttgc tccagcaact gttgctgagc ggttggtgct gcctcgacca tcggcatgac
    21841 ggccagaccg agggaaagcg tgaatatgtt tagtgtgaat ttgcgcattc ctggccccag
    21901 tcaggtaata actcaccttt tgtcgagaag cggaaacggt gttgatccca gccttgtcca
    21961 aacagggtca gcacatagtt gtaataggca tcgctgccgg gaaagttatc ggccacgcgc
    22021 tggcgctgaa cggcctgcgc atcgcggttt tgtaaaaagg gcagcatggc ggcagaaaaa
    22081 ccgaccggtc ctttaccctg cgctttcccc gtagccacat ccactttttc cggcggataa
    22141 ccgtttttct cagtgaatgt cgccatcggt ttaaaccggt tgagcatccg cgctttttgc
    22201 ggatcgctgt caggcatcat gcctacccac atgtaaacgc ggatagcgtc gtagctgctg
    22261 atcaatgttt tttcggcttt tagctgccag cctttgtctt tctcatagcg cacccagtct
    22321 ggcgaaaaac ctttcggggc ggtttccagc aataaacgtt gattggtttc gcgcagcgtg
    22381 gtccacggcg cgccaaagcg ggtgaaatac tgcgccagcg tcggcggcag gtagctgggg
    22441 ttaaaacgcc agctgttatc ctcagcaaaa cccactttgc ccggtaacaa catggaaccc
    22501 agcccaggca ccgtcaccac ttcctcccgc gcgatacgtt ttagcaacgc gctgccgatg
    22561 tcggtataac gctgctcttt ccacaaacgc cccgcctcca gcaacgacca ggccatccag
    22621 acatcaccat cggaggccga attgctgtcc agcacttccc acttactgtt ctctttcttg
    22681 ccccacagcc aggcgggcaa acgttctttt aaagaaccct gagcgagatt gttctgcgtc
    22741 cagtcgagaa tattatcgaa agctgcacgg tcgttagccg ccagggcaaa gaacatgccg
    22801 taactttgcc cttcggaggt ggtgattttg cgcgcgtcgc tggggtcgat gacgcgccct
    22861 tcctgactga tgtaatcctt tttaaactgc tcccaggcag gccaggtaca ggctgcctga
    22921 acactaaagg cagccagcag cagcatcgtc acgattccac tacgcaacac attcatcttc
    22981 aattactcgt tatccgggtt aagacgacga cgactaataa ttcgcagcag acgccacagt
    23041 acccatgcca gcaatatcac actgatagcc gccagcaccg ccagcagaat cggatggttt
    23101 gccagcgcat accacacgcg ctcgaaccac ggcagatgac ctacgtaata aacgtcgcca
    23161 acacgtaggc tgttgatacc ggactcgcgg atcaccgcga ccgaaccgaa catggtggcg
    23221 cgtttgccgc tatcgttcac cgcatcgtta agcatttcat aaccgcgtgg gctatctgcc
    23281 aacagcgcaa tcacgctgcg ctggtcgtta tacggcgact ggaagccaat caccgccgcc
    23341 atcgcaccgg aagaggtcag cgttgaccgg gtttctgccg cgcgatcgct ctcgtccggc
    23401 acaatgccgg ggaacggggt ctggcgcatc ggtgttttca cccagctttc ggtcgcctgc
    23461 accaataggt cgatctgctt atcgtctttc agtttgtccg ggataccacc gatgatcatg
    23521 atgtcggcat ctttgccctg aatggtgctg ccatcatcgg tcaccgtcag attaatcgcc
    23581 gggaagcccg tctgtgcgcc gataaaacca acagtattca gcaacgtttc catctgtgct
    23641 tcgttaggcg ctttcggcat cacggtgatg gtttgcgaca gatccgccat ccggctgaat
    23701 gggaagcccg cgttagcaaa ggcgcgtaga tccggcatcg ggatgaagtg gtaatacttc
    23761 gagaagtcga tggtggagtc gtcaccaatc accacatgat tctgcaccgg ctggaaggta
    23821 atacagttat ccaccgaacc gcccggcatc gggttcatat actcaaagtc gaagcgcagc
    23881 tggttggtcg cgcccagttt cagcgccgga atagagacat ctgttttgcc atccagcaaa
    23941 ccttgtaata ccggaatccg cagcagcagg cggttcgcct cctgtttgct gctcaggttg
    24001 aaggattgca ggaactggtt attcaggctg atatccatcc gcgaactgtc tttcaccggc
    24061 ggcatggtgt agcggtaatt aatatccata tcaatgccgg tactgcgcat caggtagaga
    24121 tccggcggca ggtttagcga aacgttaatc gctgctggct caagaccgct ggattgtaac
    24181 tgttcttcat aggttttcag ttcgccaaat gtgaccggac gatcggtacg tacccagttc
    24241 ggcgcatcgt acggcttacg cggtagcagc ggtttcactt cattcactac cacgctttca
    24301 ccacggaaca gaatgttacc ctgagcgata cctttcgctg cctgcaacag gtctttgtcg
    24361 tcacgaccaa acaccaccag cagtttgacg taaggattct gcggatggtt aatcatctca
    24421 atcacagggg cttttaccgc cggatgatcg cgcaggaagt ccggccgttt gtcgttggtt
    24481 gcaaagacaa tggcattacg atccggcagt tggttataga gcaccgggaa gttctgccca
    24541 cgccagccag aacgcgaacc aaaccacgag gcgacaatgg cagaggcttg ttgcagccca
    24601 acatccggcg cacccgcaaa gaccatcggc aaggtgttag tacggttatc gcgcgggtca
    24661 aagaatggca ccgggaagtg tgacaggtca ttcttcacat tcagggtctg ataggtcaga
    24721 tccagtccac tgctccgccc aacatccagc caaagcgtgg tgctggccgg gttttcgcac
    24781 acgtcctgat aatggccgac aaactccagc cgcacacggt tgaagtcggt aataaacagt
    24841 gggttaatgg gcatttgcgc cagcgttttt ttacccaact gttctttggt cactggcagc
    24901 acgcccatca gctcatcatt gagataaacc tttaactgcg actggacagg cagtaacgat
    24961 ggcgatggcg tgtattcgag gttgagcatc gccttcgtca ccacttcatc gctgcgcata
    25021 ccaaactcaa tgctgccgtt cgggttaatg ccacgtagca ccatgctgcc cggcggcggc
    25081 gcaatttgcg caaaggtcag cttcacatca cgcgaaggac cgttctgcgc cacgactggc
    25141 gcatcagcgc cctgcacgcc cggcatcact tgccccacct gcggattttg ttccgtctgg
    25201 gcggctacag ctggctcagc attgatcagt ggttgcgttg ctggcgtcgc ctgcgtcatg
    25261 aaagagggga acgcactcat ccccatagcc actgcacaaa tccagaatag ttttcttttc
    25321 atcgcgttat catcattgtt gagccaaagc ctgatccgat ggttgtgccg tttcgctccg
    25381 ctccgggcgg cgcggaataa acgatacaac ccaggaaacc agagaagtca gcacacggaa
    25441 tatgcccttc accgaagaag gcgcaaactc cgccagatgg cggtagccac ggaagccgag
    25501 cttcagaata tccagcagac tttccagcgg cttatcttcc gggtagctgt cctgccagag
    25561 cgcccatgta tccgcacggg caaacgtaca ctgcacaaaa tcgatatgtt gctgggtggt
    25621 gagcggcatt aatttcagcc caacttcatt acccatcacg cgcgccacct gggtcgggaa
    25681 gacgtattcc tgctgaccgc gtttaagcaa cagattcact ttctgccctt ccagaatctg
    25741 cgcctgaccg ttgatcttga tccccaaacc accgtcggag aaatcctgaa cggtacacga
    25801 gaagaggtga ccatcttcgc gggcaattgc cgcgggcatc gtcatctcca cgcggtgcga
    25861 tcggcgtacc tgtttgcttt ctaccgatac cgcaactgcg ccgccaagaa caatcaggtt
    25921 gtagaacacc cacaccatac tgacgaccac ggtgagcatc tcggttggcg ggccatagaa
    25981 gtagcgccag atgcctaccg caacgcccac caggttgagt agaacaagga agatgtaggg
    26041 ccgcgagatc acccagtcga cgtactcttc ttccaccagt ccacctttgg cggtgacgtt
    26101 aaatttgcct ttgtgcgggt taatcagcgc caccagcgtc ggtggtgcga tataccacgc
    26161 cagcaccgtt tcgtagattt cactccagaa agagtggcga tatttgccct ggatcttcga
    26221 gttggtcagg ctggcatgga tcatatgcgg cagcacgaac agggcgatca tcaacgctgg
    26281 cgcatagatg atgtaggcat gaagcagcag gaacgccagc ggcgcagtca ggaagatcag
    26341 tcgtggaatg cccgacaaga agtggaacat ggcgttgacg tagcacagcc gctgggcgaa
    26401 tttcagccct ttaccggtaa gcgggttatc gagacggaag atttgcacca tcccgcgtgc
    26461 ccagcgaata cgctgaccga tatgcgccga cagactttcg gtcgccagcc ccgccgcctg
    26521 cggaatacgc atatatgcag aggtgtagcc acgacggtgc aggcgcagag acgtgtgcgc
    26581 atcttcggtt accgtttcga cagcaatacc gccaatttca tccaatggtt tgcgacgaat
    26641 tactgcacag gaaccgcaga agaaagtggc gtcccacata tcgttgccat cctgcaccag
    26701 accatagaac agcgtgcctt cgttcggtgt tttacgaaaa cgccccaggt tgcgttcaaa
    26761 cgggtccggc gagaagaagt ggtgtggagt ctgcatcatc gccagctgtt tctctttcag
    26821 gaaccagccc atggtcattt gcaggaacga tcgcgttggt acgtggtcgc agtcgaaaat
    26881 cgacacgaac tcgcctttgg catatttcag cgcattgttg atgttgcccg ctttcgcatg
    26941 ttcatgagtg gtacgggcga tatatttcac ccccacgttt tgcgcaaact ggcgaaactc
    27001 ttccctgccg ccgtcatcga ggatccagat gttcagtttg tctttcggcc agtcgatacc
    27061 cagcgaggcg taaatggtat ttttcaccac gttgagatct tcgttgtaag tcgggacaaa
    27121 gatatccacc gacggccaca gcgacatatc tttcggcaat ggcaccggct gacgattcag
    27181 cggccatact acctggaagt agccgagcac cagcacaatc cacgcgtacg tttcagcgaa
    27241 gagcagaata agcccgcaca ccaggctgac cggatcgtcc cagttcagcg tagaggtgta
    27301 acgccaccag atataacggc aagaaacggt cagcgacagc acaatcaaca ttagcgccga
    27361 gaagcgcccc ggcatccgcc gtacgatcag cgctaccccc cacagcagca tcaggaagat
    27421 aaactgcgcc agcgggttaa acggctgagt aacgcagatt aacgccagaa tcagcgagaa
    27481 ggtgacgatg ataccgagga tcaaccgccg cgccccggca ctcaaatgac cgagctcttt
    27541 tttctcatcc agatgctgtg ttttatggct aacgcgctca ggcagctcgt tcatccattg
    27601 atggtaacgt ccacgaatat tttgcagacc tgaaaatgcc cgcctgcgcg gtttcggcgt
    27661 ttctttgcgc gatgcaccga tcaataacca gcatgtttga atgagataac ggaccgggtc
    27721 cagcggacgc ggacgcgagg cgttgatatg cggatacagg tttttatgtt ctgcgcgaat
    27781 acgctgccag cgcgggtgct ccagcggaat aaaaatccag gccaggatca tccagaaaca
    27841 gccgagcgtc gcgctgaaag ccgacgcacc gtgacgacga taatcgcgat aacgcccgat
    27901 aagccgcgcg ttgaccggcg ggataagcaa ccaccgggtc aggatactca tgatgcactc
    27961 ccgactggcg ttttcagccc ggagtagttc aacaggcacc agttcgccag cgtcagtatc
    28021 tcttcagccg ccagcgcatc actgcgatat tcacctactg gttgcttagc cgccaggcat
    28081 tcagccatcg cttcatcacg atgaatgagc atcggcagta atcggcgctg gctttgcaac
    28141 caaagctggt aaatatcgtc ctgaacctga ctgccaatac ggaagtcatt aatcaaaata
    28201 tgtgcgccat ccggcagcgc ttgctgatgc agtcggatat ggcagttggc atccacattg
    28261 acgattgcca gcgagtgatc gcacaaactc agcagctggt gggttatctg cgaggcatca
    28321 cgcggtaagt cgattaaaat ccactggtaa cgcccgctgg cttttagttg ctgtaagccg
    28381 gagcaaatat cgctcagccg ggtttgccag tgctgtggat tttcttgttc ttcaatggat
    28441 aactgaccaa aaggcagcaa atcgagctgc gaggtgtagc gcaacccagc gtcacgccag
    28501 tcctggccat ccagcatcgc tctggcccag ccctgacggt gggtaaaatc aacattaaat
    28561 gacaggcgca acaagttgtc cgggcaggca tcgaccacca gaacattttc tcccaacatt
    28621 tgtaatgacc aggctaatgc ggcggtgatg g
//
LOCUS       DAZGAN020000054        28370 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGAN020000054 DAZGAN020000000
VERSION     DAZGAN020000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.054"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            259..930
                     /locus_tag="ACHKYO_RS13135"
                     /old_locus_tag="pgaptmp_002627"
     CDS             259..930
                     /locus_tag="ACHKYO_RS13135"
                     /old_locus_tag="pgaptmp_002627"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HGU4429454.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            940..2136
                     /gene="yaiP"
                     /locus_tag="ACHKYO_RS13140"
                     /old_locus_tag="pgaptmp_002628"
                     /pseudo
     CDS             940..2136
                     /gene="yaiP"
                     /locus_tag="ACHKYO_RS13140"
                     /old_locus_tag="pgaptmp_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2114..2695
                     /locus_tag="ACHKYO_RS13145"
                     /old_locus_tag="pgaptmp_002629"
     CDS             2114..2695
                     /locus_tag="ACHKYO_RS13145"
                     /old_locus_tag="pgaptmp_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU4429455.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIESEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2697..3470
                     /gene="yaiO"
                     /locus_tag="ACHKYO_RS13150"
                     /old_locus_tag="pgaptmp_002630"
     CDS             2697..3470
                     /gene="yaiO"
                     /locus_tag="ACHKYO_RS13150"
                     /old_locus_tag="pgaptmp_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HGU4429456.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3655..3930
                     /gene="frmR"
                     /locus_tag="ACHKYO_RS13155"
                     /old_locus_tag="pgaptmp_002631"
     CDS             3655..3930
                     /gene="frmR"
                     /locus_tag="ACHKYO_RS13155"
                     /old_locus_tag="pgaptmp_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HGU4429457.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3965..5074
                     /gene="frmA"
                     /locus_tag="ACHKYO_RS13160"
                     /old_locus_tag="pgaptmp_002632"
     CDS             3965..5074
                     /gene="frmA"
                     /locus_tag="ACHKYO_RS13160"
                     /old_locus_tag="pgaptmp_002632"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HGU4429458.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5167..6000
                     /gene="fghA"
                     /locus_tag="ACHKYO_RS13165"
                     /old_locus_tag="pgaptmp_002633"
     CDS             5167..6000
                     /gene="fghA"
                     /locus_tag="ACHKYO_RS13165"
                     /old_locus_tag="pgaptmp_002633"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU4429459.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6125..6664)
                     /gene="yaiL"
                     /locus_tag="ACHKYO_RS13170"
                     /old_locus_tag="pgaptmp_002634"
     CDS             complement(6125..6664)
                     /gene="yaiL"
                     /locus_tag="ACHKYO_RS13170"
                     /old_locus_tag="pgaptmp_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HGU4429460.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6766..7977)
                     /gene="mhpT"
                     /locus_tag="ACHKYO_RS13175"
                     /old_locus_tag="pgaptmp_002635"
     CDS             complement(6766..7977)
                     /gene="mhpT"
                     /locus_tag="ACHKYO_RS13175"
                     /old_locus_tag="pgaptmp_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HGU4429461.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKSILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8252..9265)
                     /gene="mhpE"
                     /locus_tag="ACHKYO_RS13180"
                     /old_locus_tag="pgaptmp_002636"
     CDS             complement(8252..9265)
                     /gene="mhpE"
                     /locus_tag="ACHKYO_RS13180"
                     /old_locus_tag="pgaptmp_002636"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HGU4429462.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9262..10212)
                     /gene="mhpF"
                     /locus_tag="ACHKYO_RS13185"
                     /old_locus_tag="pgaptmp_002637"
     CDS             complement(9262..10212)
                     /gene="mhpF"
                     /locus_tag="ACHKYO_RS13185"
                     /old_locus_tag="pgaptmp_002637"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HGU4429463.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10209..11018)
                     /gene="mhpD"
                     /locus_tag="ACHKYO_RS13190"
                     /old_locus_tag="pgaptmp_002638"
     CDS             complement(10209..11018)
                     /gene="mhpD"
                     /locus_tag="ACHKYO_RS13190"
                     /old_locus_tag="pgaptmp_002638"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HGU4429464.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQA
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11028..11894)
                     /gene="mhpC"
                     /locus_tag="ACHKYO_RS13195"
                     /old_locus_tag="pgaptmp_002639"
     CDS             complement(11028..11894)
                     /gene="mhpC"
                     /locus_tag="ACHKYO_RS13195"
                     /old_locus_tag="pgaptmp_002639"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HGU4429465.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSLNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSMAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11912..12856)
                     /gene="mhpB"
                     /locus_tag="ACHKYO_RS13200"
                     /old_locus_tag="pgaptmp_002640"
     CDS             complement(11912..12856)
                     /gene="mhpB"
                     /locus_tag="ACHKYO_RS13200"
                     /old_locus_tag="pgaptmp_002640"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HGU4429466.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFSQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAVKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12858..14522)
                     /gene="mhpA"
                     /locus_tag="ACHKYO_RS13205"
                     /old_locus_tag="pgaptmp_002641"
     CDS             complement(12858..14522)
                     /gene="mhpA"
                     /locus_tag="ACHKYO_RS13205"
                     /old_locus_tag="pgaptmp_002641"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HGU4429467.1"
                     /translation="MAIQHPDIQPAVNHSVQVAISGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LNTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14713..15546
                     /gene="mhpR"
                     /locus_tag="ACHKYO_RS13210"
                     /old_locus_tag="pgaptmp_002642"
     CDS             14713..15546
                     /gene="mhpR"
                     /locus_tag="ACHKYO_RS13210"
                     /old_locus_tag="pgaptmp_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HGU4429468.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLAARPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     H"
     gene            15623..16705
                     /gene="lacI"
                     /locus_tag="ACHKYO_RS13215"
                     /old_locus_tag="pgaptmp_002643"
     CDS             15623..16705
                     /gene="lacI"
                     /locus_tag="ACHKYO_RS13215"
                     /old_locus_tag="pgaptmp_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HGU4429469.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQVQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16828..19902
                     /gene="lacZ"
                     /locus_tag="ACHKYO_RS13220"
                     /old_locus_tag="pgaptmp_002644"
     CDS             16828..19902
                     /gene="lacZ"
                     /locus_tag="ACHKYO_RS13220"
                     /old_locus_tag="pgaptmp_002644"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGU4429470.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPVADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSTFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEASDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19954..21207
                     /gene="lacY"
                     /locus_tag="ACHKYO_RS13225"
                     /old_locus_tag="pgaptmp_002645"
     CDS             19954..21207
                     /gene="lacY"
                     /locus_tag="ACHKYO_RS13225"
                     /old_locus_tag="pgaptmp_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HGU4429471.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21273..21884
                     /gene="lacA"
                     /locus_tag="ACHKYO_RS13230"
                     /old_locus_tag="pgaptmp_002646"
     CDS             21273..21884
                     /gene="lacA"
                     /locus_tag="ACHKYO_RS13230"
                     /old_locus_tag="pgaptmp_002646"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HGU4429472.1"
                     /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVESSV"
     gene            complement(21987..23168)
                     /gene="cynX"
                     /locus_tag="ACHKYO_RS13235"
                     /old_locus_tag="pgaptmp_002647"
     CDS             complement(21987..23168)
                     /gene="cynX"
                     /locus_tag="ACHKYO_RS13235"
                     /old_locus_tag="pgaptmp_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HGU4429473.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPARVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPVRFPQLWVKE
                     A"
     gene            complement(23174..23644)
                     /gene="cynS"
                     /locus_tag="ACHKYO_RS13240"
                     /old_locus_tag="pgaptmp_002648"
     CDS             complement(23174..23644)
                     /gene="cynS"
                     /locus_tag="ACHKYO_RS13240"
                     /old_locus_tag="pgaptmp_002648"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HGU4429474.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23675..24334)
                     /gene="cynT"
                     /locus_tag="ACHKYO_RS13245"
                     /old_locus_tag="pgaptmp_002649"
     CDS             complement(23675..24334)
                     /gene="cynT"
                     /locus_tag="ACHKYO_RS13245"
                     /old_locus_tag="pgaptmp_002649"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HGU4429475.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            24444..25343
                     /gene="cynR"
                     /locus_tag="ACHKYO_RS13250"
                     /old_locus_tag="pgaptmp_002650"
     CDS             24444..25343
                     /gene="cynR"
                     /locus_tag="ACHKYO_RS13250"
                     /old_locus_tag="pgaptmp_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HGU4429476.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAKHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            complement(25680..26963)
                     /gene="codA"
                     /locus_tag="ACHKYO_RS13255"
                     /old_locus_tag="pgaptmp_002651"
     CDS             complement(25680..26963)
                     /gene="codA"
                     /locus_tag="ACHKYO_RS13255"
                     /old_locus_tag="pgaptmp_002651"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HGU4429477.1"
                     /translation="MSNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26953..28212)
                     /gene="codB"
                     /locus_tag="ACHKYO_RS13260"
                     /old_locus_tag="pgaptmp_002652"
     CDS             complement(26953..28212)
                     /gene="codB"
                     /locus_tag="ACHKYO_RS13260"
                     /old_locus_tag="pgaptmp_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HGU4429478.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         6788 a         7698 c         7350 g         6534 t
ORIGIN      
        1 tgcaattaat tgacaaacta aatcacatct aaatacaatg tataaaacaa aataactata
       61 cataccaaac atacaaacac ttaacatatg aattgcataa aaacattacc cagtcgaacc
      121 tccgggtctg aaagcccttg cttttcagtt attagcaaca tttaacattt ataaattaac
      181 atctggctgc gccagagatt acgtctctga gtgcatcgtt gtgcttgtag tataataaat
      241 aaaatggagt gtcattaagt ggataaggtt ttagattcag ccctcctttc ttcggcaaat
      301 aaaagaaagg gtatccttgc tattggcgca catcctgacg atatagaatt aggctgtggc
      361 gcatcgcttg ctcgtcttgc gcaaaaagga atttatatcg cagcagtggt gatgactacc
      421 ggcaactctg gtacagatgg aataatcgat cgccatgaag aatcgcgcaa cgccctaaag
      481 atattagggt gccaccaaac tattcatctt aattttgctg acacccgcgc tcatttacag
      541 ctcaatgata tgatttccgc cctggaagac atcattaaaa atcaaattcc ttctgatgtt
      601 gaaatcatgc gggtatatac catgcatgat gccgaccgcc atcaggatca tctcgctgtt
      661 tatcaagctt caatggttgc ctgccgcact attccacaaa ttctcggcta cgaaacccca
      721 agtacctggc tttcatttat gcctcaggtt tttgaatccg ttaaagaaga atatttcaca
      781 gtcaaacttg ctgcattaaa aaaacataaa agccaggaac gacgtgatta tatgcgtcat
      841 gatcgcctgc gtgcagttgc acaatttcgc gggcaacagg tcaatagcga tctgggtgaa
      901 ggctttgtaa ttcataaaat gattctttga gcaaattgca tgaaaacctg gatatttatc
      961 tgtatgtcca tagcaatgtt gctatagttt ttaagtacgc taagacgtaa acccagtcaa
     1021 aagaaaggct gtattgacgc cattatacct gcgtataact aaggcccgtg tctggcgcag
     1081 tcactggata atctactgcg taacccttac ttttgccggg taatttgcgt taacgacggc
     1141 tccacggaca ataccgaagc ggtcatggcg gaagtcaaac gcaaatgggg cgaccgcttt
     1201 gttgccgtca cgcaaaaaaa taccggtaaa ggtggtgcgc tgatgaatgg cctcaattat
     1261 gccacctgcg accaggtttt tttaagtgat gccgacacct atgttccgcc cgatcaagac
     1321 ggaatgggct atatgctggc agaaattgag cgcggtgctg atgccgtagg cggcattccc
     1381 tctactgcgt tgaaaggcgc ggggctgtta ccgcacatcc gcgcgaccgt aaagttgccg
     1441 atgattgtta tgaagcgcac gctacagcag ctcctgggcg gcgcaccgtt tattatcagc
     1501 ggtgcctgcg ggatgttccg tactgatgta ttgcgtaagt tcggtttctc ggatcgtact
     1561 aaagtcgaag accttgatct cacctggaca ttagtggcaa acggctaccg tattcggcag
     1621 gcgaatcgct gcatcgtata cccacaggaa tgcaacagtc cgcgtgagga gtggcgtcgc
     1681 tggcggcgtt ggattgtggg atacgcggtc tgtatgcgcc tgcataaaag acttttattt
     1741 agccgcttcg gtatcttcag tatatttcct atgctgttgg ttgtgattta tggcgttggg
     1801 atttatctca ctacctggtt taatgaattc atcaccaccg ggccgcatgg tgtggtgttg
     1861 gcaatgtttc cgcttatctg gatcggcgta gtttgtgtta ttggtgcttt tagcgcctgg
     1921 tttcatcgtt gctggttgtt ggtgccttta gcgccgcttt ccgttgtgta tgtattatta
     1981 gcttatgcca tctggattat ttatggactt attgcctttt ttactggacg cgaacctcag
     2041 cgcgacaaac ccacccgcta ttccgcactg gtggaagcgt caaccgctta ttcccaacct
     2101 tctgtcacag gaactgaaaa actatctgaa gcttaatgtt aaagagaaaa atattcagat
     2161 tgcagaccag gtaattattg atgaaactgc aggtgaagtt gttatcggcg cgaatacccg
     2221 tatttgtcat ggtgccgtta ttcagggtcc ggtagtgatt ggcgcaaact gcctgatagg
     2281 taattatgcg tttattcgtc ctggcacaat aatcagcaat ggtgtaaaaa ttggttttgc
     2341 caccgaaatt aaaaatgcgg ttattgaatc ggaagcaacg attggtccgc aatgttttat
     2401 tgccgactcg gtagttgcaa accaggcata tttgggcgca caagtacgta ccagtaatca
     2461 tcgtctggat gaacagcctg tgtctgttcg aactccagag ggaattatcg ctaccggatg
     2521 cgataaatta ggttgttata tcgggcagcg ttcacgcctt ggcgtacaag ttataatttt
     2581 gcctgggcga attatttccc cgaacacgca acttggcccg cgcgtgattg tagaacgtaa
     2641 tttacctgcc ggaacttatt cactccgaca agaacttatc cgtacaggag attaaaatga
     2701 taaaacggac gttattagcg gcggccattt ttagcgcatt gcccgcttat gccgggttaa
     2761 cttccattac cgcgggctac gattttaccg attattctgg cgatcatggc aaccgtaatt
     2821 tagcgtatgc tgaactggtg gcgaaagttg aaaacgcaac gctgcttttt aatctttccc
     2881 aggggcgtcg tgattatgaa actgaacatt tcaatgccac tcgcggtcag ggtgcggtct
     2941 ggtataaatg gaataactgg ctgacaaccc gaacgggtat tgcctttgcg gataatacgc
     3001 cggtctttgc ccgccaggat tttcgtcagg atattaacct ggccctgttg ccaaaaacgc
     3061 ttttcacgac cggttatcgc tacactaaat attacgatga tgtcgaagtc gatgcctggc
     3121 aaggcggcgt atcactctat actggcccgg tgatcaccag ctaccgctat acccattatg
     3181 actccagcga tgcaggtggt agttatagca atatgatttc cgtgcgtctg aatgacccgc
     3241 gcggcactgg ttatacgcaa ctatggctaa gccgcggaac aggtgcttac acctatgact
     3301 ggacgccaga aacacgctac ggcagcatga agagcgtcag tctgcaacgt attcaaccgc
     3361 tgactgagca acttaatctc gggctgacgg caggtaaagt gtggtacgac accccaaccg
     3421 atgattataa cggtctgcaa cttgcagccc atctgacctg gaaattctga ttccttctgc
     3481 cgcccgctat ccggggcggc cttccctgcc gattagcccc ccctttcctc tttgttttcc
     3541 gaccacattc accggataaa ttttattctc cagtgttata tactataggg gggtatgcat
     3601 tgacatatag catacccccc tatagtatat tgcgtgcaga taatgaggtg cgaaatgccc
     3661 agtactccgg aagagaagaa aaaggtcctt actcgagttc gtcgtattcg agggcagatt
     3721 gatgctctgg aacggtcgct ggagggtgat gccgaatgcc gtgccatact ccaacagatc
     3781 gctgccgttc ggggcgcggc taatgggctg atggcagaag tgcttgaaag ccatatccgg
     3841 gaaacgtttg accgaaatga ctgctacagc cgcgaagtca gccaatccgt tgacgacact
     3901 attgaactgg ttcgagccta tcttaaatag ctgaatctat taccatattg aggaagagcg
     3961 agagatgaaa tcacgtgctg ccgttgcatt tgctcccggt aaaccgctgg aaatcgttga
     4021 aattgacgtt gcaccaccga aaaaaggtga agtgctgatt aaagtcaccc ataccggcgt
     4081 ttgccatacc gacgcattta ccctctctgg ggatgacccg gaaggtgtat tcccggtggt
     4141 tctcggtcac gaaggggccg gcgttgtggt tgaagtcggt gaaggcgtaa ccagcgtcaa
     4201 acctggcgac catgtgatcc cgctttacac cgcggagtgc ggcgagtgtg agttctgtcg
     4261 ttctggcaaa actaacctct gtgttgcggt tcgcgaaacc cagggtaaag gcttgatgcc
     4321 agacggcacc acccgttttt cttacaacgg gcagccgctt tatcactaca tgggatgctc
     4381 aacattcagt gaatacaccg tggtcgcgga agtgtctctg gccaaaatta atccagaagc
     4441 aaaccatgaa cacgtctgcc tgctgggctg tggcgtgacc accggtattg gcgcggtgca
     4501 caacacagct aaagtccagc caggtgattc tgttgccgtg tttggtcttg gcgcgattgg
     4561 tctggcagtg gttcagggcg cgcgtcaggc gaaagcggga cggattatcg ctatcgatac
     4621 caacccgaag aaattcgatc tggcgcgtcg cttcggtgct accgactgca ttaacccgaa
     4681 tgactacgac aaaccgatta aagatgtcct gctggatatc aacaaatggg gtatcgacca
     4741 tacctttgaa tgcatcggta acgtcaacgt gatgcgtgcg gcgctggaaa gtgcgcaccg
     4801 cggctggggt cagtcggtga tcatcggggt ggcaggttct ggtcaggaaa tctccacccg
     4861 tccattccag ttggtcactg gtcgcgtatg gaaaggttcc gcgtttggcg gcgtgaaagg
     4921 tcgttcccag ttaccgggta tggttgaaga tgcgatgaaa ggtgatatcg atctggaacc
     4981 gtttgtcacg cataccatga gccttgatga aattaatgac gccttcgacc tgatgcatga
     5041 aggcaaatcc attcgaaccg taattcgtta ctgatttccc gcaggtatac cccgtccact
     5101 tcagacgggg ttcttaatac tctccctggg cagccgtccg ggggattaac cgtgagataa
     5161 tgactgatgg aactcattga aaaacatgcc agctttggcg gctggcaaaa tgtgtatcgg
     5221 cattattccc aatcactgaa atgtgaaatg aatgtcggcg tctatctccc accaaaagcc
     5281 gcgaatgaaa aattgccggt gctgtactgg ctttcgggcc tgacctgcaa cgagcagaat
     5341 ttcattacta aatcggggat gcagcgttac gcggctgaac acaacattat tgttgttgcg
     5401 ccggacacca gtccgcgagg cagtcatgtc gcagatgccg accgttacga tctcgggcaa
     5461 ggtgccgggt tttacctgaa cgcaacgcaa gcgccgtgga atgaacatta caaaatgtat
     5521 gactatatcc gcaacgagct gccggattta gtgatgcaac attttccggc aacgaccaga
     5581 aagtctatct ctggtcattc tatgggcggg ctgggcgcgc tggtgctggc gttacgtaac
     5641 ccagatgaat atgtcagcgt ctcggcgttt tcgcccattg tctccccatc gcaagtaccg
     5701 tggggacagc aagcctttgc cgcatatctt ggtgaaaata aagatgcctg gttgaattac
     5761 gatccggtga gtcttatttc acaaggtcaa cgcgttgcgg aaatcatggt tgatcagggg
     5821 ttgagtgatg atttttacgc ggaacagctg cggactccaa atcttgaaaa gatctgccag
     5881 gagatgaata tcaagacgtt aatccgttat cacgagggtt acgaccacag ctattatttt
     5941 gtctccagtt ttattggcga gcatattgcc taccacgcca ataaactgaa tatgcgttga
     6001 taatagtgca cgactgccgg atgcggcgtg aacgccttat ccgcctacat ttcgcccgca
     6061 ggccgtaggc atgataagac gcgcatagcg tcgcatcagg catctgcgca cgacttatct
     6121 atgtttacca catcaaatca tcaggcactt tgaagtcggc atatgggtcg tcttcatcct
     6181 gctcttcggc actgagcgcg ctgtgcaaca caatactgct ggcatcacgc tgagcaattt
     6241 tatcggcaac gctggcggga ataatggcgt attcaccttc gctattgtta tcgaccaaca
     6301 agcgggcaat cgccagacga ccattaatca actgcgcctg tgtgagctta tcgacaaaaa
     6361 tcttcttaat cagattgccg tcagtgaagt taaaaccaat atcaccattg gcaatggtga
     6421 ttcggttcat ttcaataagc tgcttcacct gggctttata ttctttcgcc aacgccgctt
     6481 gtttttgctg ttcgctaagc tgtttatcac gctcaagctg tgccttttta ttttcttcta
     6541 ccgccgcccg agcttcacgc gcctgaacgc gcgatttttt cgccgttctc tcgaccttcg
     6601 ccgctttttt gctggtgact aatcctgctt tgagcaactg ctcttgtaag gtaagttttg
     6661 ccatctttgt ttccaaaccc gctgaataat ttttagcgag tatacctgta aacgccgtgg
     6721 gtgtattggg ggcaagtccc tttcgcccct gctaaataag gcgcgtcagg catcggcaca
     6781 cggctgcatt cttgatctcc gactcatcaa aataaacacc gccaacccag caacaagaat
     6841 acccggtgca gaagcggcca ttacgccgac cgtgccagtg cctaatgcca gcattttccc
     6901 ggccagtaac ggaccgctca tagcccccag acgccctacc gccacggctg ttcccacacc
     6961 tgttgcgcgg atctgcgaac tgtaaaacaa cggtgccagg gcatacaaaa cgctttgccc
     7021 acctgtcgca aacaaccccg cgacaaatcc cgccagcaac ataccgttaa atgacgacac
     7081 cgttccaagc gccagcagcg aagctaacat gccgctataa atcagtagcg acatggttac
     7141 tggacgcagc ttatccatca atgcgcccaa cattaacgtc ccgcttgccg cccccatttg
     7201 cagggcaaac atcacccctg ccgcctgcga tggctggaat ccttgctcca ccaaaagtag
     7261 cggtagccag ttgatcaaca tataaaccac cagcagagtg aagaaataac acaaccacag
     7321 cagcagcgtc gcggttgccg tttctggcgc aaataaggca cgcagtggtg gcgcagcctg
     7381 tttttcgcca gcgaaaaccg ctgactccgg cagccagcgc attaatagcg gcaccagaat
     7441 caacggcacc acaccaccta cccaaaacac cgtttgccat gctaagtttg cccccgcgaa
     7501 acccagtgtc gccgccagcg ccgcgccaat gggaacaccg caatacatca ggctcactgc
     7561 cgtcccacga aaacgtggac ccgcggcttc agacgtcagg gcgataagat tcggcaacgc
     7621 cgcccccagc ccgacaccgg tcatcagccg cgcaaagacc agtgagggga aatcccaggc
     7681 aatcgccgtt gccagtgaga acaaaccaaa cagcgcaact gagccaatca aaatactctt
     7741 acgaccataa cggtctgcca gcatcccgcc aaccaacgcg ccgggtagca aaccgagtat
     7801 tccggcgcta aatatccagc ccatttgcat tttatcgagt gcgaaagcct gggcgatgcc
     7861 acccgccgca atgccagccg cctgaagatc cagcccttcc atcagagcga ccaaaaaaca
     7921 aagcccgatg gtcagcatca ggcgggatga agatgatgaa ggggtacgag tcgacataag
     7981 atgaacctcg ttgcgagtgc aggtgaatcg cgactgatac attgccagcc gcgtaatcag
     8041 aactatttaa tgactgccta cgggcgaaat gtaggccgga caagacgttt atcgcatccg
     8101 gcagtcgtgt gcagatgcct gatgcgacgc taacgcgtct tatcaggcct acaaattctc
     8161 acgccacccg taggccggat aaggtgttta cgccgcatcc ggcagtcgtg ctgggatggg
     8221 catacaaaat gcacgttctc ggcagtcatc attatttgtt gttgcgcaga tccagcgcca
     8281 cgtcaacgat catatcctcc tggccgccaa ccatccggcg tttgcccagc tcaacgagaa
     8341 tatccaccgc acttaagcca taacgcgccg ccgccgtttc acagtgacgc aggaagctcg
     8401 agtaaacacc agcgtatccc agcgccagcg tttcgcgatc gactcgtacc ggtcgatcct
     8461 gcaacggacg caccaggtcg tcagcggcat ccattaacgc atagagatcg gtcccatgct
     8521 gccagcccag tttatccgcg gcggcaataa acacttccag cggtgcgtta cctgcgcccg
     8581 cgcccattcc cgcgaggctg gcgtcgattc ggtcgcagcc ctcttccacc gccgcgatag
     8641 agttcgccac gccaagactc aggttatggt gagcgtgcat gccagtttgc gtttctggtt
     8701 tcagctctgc tttcagggcg cggaaacggt cacggatatc gctcatgttc atcgcaccgc
     8761 cagaatccac cacataaata caggtcgcac cgtagccttc catcagcttt gcctgctttg
     8821 cgagattctc cggcgtggtc atatggctca tcatcagaaa accaacggtg tccattccga
     8881 gctcgcgggc atactgaata tgctgggcgg aaacatcagc ttcggtacag tgcgttgcca
     8941 cacgaaccac ccgcgcgcca gcctgccagg catttttcag atcgtgaata gtgccgattc
     9001 ctggcagcaa caacgtcgcg attttggcgt gcttcaccac atccgccgct gcttcaatcc
     9061 attcaaggtc gctatgtgcg ccgaaaccat agttaaagct ggaaccctgc aaaccgtcgc
     9121 catgggccac ttcaatcgaa tccacgcggg catcgtccag tgctttggca atctggcgaa
     9181 cgttttccag cgaatactga tgacgaatgg cgtgcatacc gtcacgcaac gtgacgtccg
     9241 agatataaag ttttttaccg ttcatgccgc ttctcctgcc ttgcgcgcca gtgactgggc
     9301 cattttttcc gctgtcgcca gcgcactgga agtcataatg tcgaggttgc ccgcataggc
     9361 aggcagataa tgcgctgcgc cttcgacttc cagccagacc gctgttttca gtccggagaa
     9421 ttgccccacg cccggtaaat tgaccggttt atcctgcggg ataacttcaa actgcacgcg
     9481 ctgtttcagg cgataacccg gtacgtaagc ctgcaccgcc tcagccattt cattgattga
     9541 ggcttcgata tcatcttgtg aagcttcgtc gctcaataca tacaccgtgt cacgcatcat
     9601 caacggtggc tctgctgggt taagcacaat aatcgccttc ccttttgccg cgccgcccac
     9661 cacttcaatg gctcgggaag tggtttccgt aaattcatcg atattggcac gcgtgccagg
     9721 tccggcagat ttactggcga tagaagcgat aatttcggcg taatgaacac gcgccacgcg
     9781 tgaaactgcc gccaccattg gaatggtggc ctggccgccg caggtgacca tgttgacgtt
     9841 cagttgatcg acgttcgcct cgaggttaac caccggcacg cagtaagggc cgatggcagc
     9901 aggcgtcagg tcaattaagc gaatatccgg tttcgcttcg cgtaaagcgg catcgttttt
     9961 cacatgcgcg cccgcgctgg tcgcatcaaa tacaatgtcg atatcagcaa attcaggcat
    10021 gttcatcagc ccgatcaccc cttcatgggt ggtggcgacg cccatacgtc tggcacgcgc
    10081 cagaccgtcg gactgaggat caatgccgac catcaccgcc atctccagat gctgaccgtg
    10141 acgcaaaatt ttaatcatca gatcggtgcc aatgttgcca gaaccgataa tggcgacttt
    10201 acgcttactc atgacagact tccttttggg gctgcgcttg aaaatgtcgc agcaactgaa
    10261 cctatgcctt caatatgggc ttcaaaacga tcgcccgcat tcactgccac catcggacct
    10321 aatgccccgg taagaatgat atctccggcg cgcagcggtt cacccaggct ggccattttg
    10381 cgtgccagcc agacggcggc attaagcgga tgtcccaggc attcgctgcc gcgcccgctg
    10441 gaaacctctt cgttattacg cgtcatcttc atggcgcagt ttttcaggtc taaccccgcc
    10501 ggacgttgcg ccggaccgcc gatgacatac accccgcagg aagcgttatc tgccacggta
    10561 tcgacaaact gaatcgacca gtcgcgaatg cggctaccca ccacctccag cgccggaagt
    10621 acccattcaa tggcgttata cagttcgtcg aaggtgatat cggttgcggg caaatcgcgg
    10681 ttcaacacca gtgcgatctc cgcttcaatg cgggcttgca gaacacggga aaaaggaatg
    10741 atttcgttat cgccataaca catgtcggca aataatgtcc caaaatccgg ttgatcaacg
    10801 cccagttgtt gctgcacttt cggatgcgtc aggcccactt tacgccctac cacgcgacgc
    10861 ccctgcgcaa cgtcatattg cacatttatg tgctgaatgg cgtaggccgc ttcagcgtta
    10921 tcgataccaa tcagatcgcg cagcggcgca atcgcttcgc cctgctctgc ggcgcggcgt
    10981 aaatccgccg ccagttgctc aagagtatgc ttcgtcatga ccattcctta agggcgtgcg
    11041 aggaaattca gcaccaattg attgaaagcg tcggcatgtt cccactgcgc ccagtgacca
    11101 cagtcgcgga agatatgcag ttcagaaccg gcaatgccgg acagcagacg cagacccgca
    11161 tccatcggca caaagcggtc gttgcgcccc cagacaatca gggtttgcgc tttgatttcc
    11221 gccagacgtg ggccaaaatc cgggaactgt ttcggattag cttccaggct cttaacgaag
    11281 ttttccaggt gatcgcggcg cgacagcata ttattcaggc gcgcttcaaa cagggcgtcg
    11341 gtcaaatcgc tggtatcgaa aacgaagata tccatcatca gcttcaggtt ttcgatagtc
    11401 ggctgacgat aaagctgatt cagtcgctta ataccttcgg ttggcatcgg cgtaaacaaa
    11461 ctcatgccgc ccgtaccgcc gcccatcagc accagtttgc cgacgcgctc cggccagtta
    11521 agggtgaacg ccatagaact atggccgccc atcgagttgc ccagcaggtg gattttggcg
    11581 atatccagtt gatccaccac gcttttcagg attcgtgcat taagatccga tcgtgaacca
    11641 ctattaacga tcgaatcgct tttgccccaa cccggacaat ccagcaggat tacccgatag
    11701 cccgcctcta ccagcggatc gatattgagg ctgaagttcg cccagccagt agcacccggg
    11761 ccggaaccgt gcagcaggac aacggtttcg tcgccttgtc cgcagtcatt aaaatggatg
    11821 cgcagcgttt tacccgcttc ttctacattc agaaaacggc tggtggcggc ttcggtttgt
    11881 ggctgataac tcatcatctt ctcctgcata ttcagttctc tgttctggcg cttaacgagc
    11941 caaatccggc aatccactcc gggattgggc ggtaataacg cccttcgcta cgccagttgc
    12001 caaatgcaga aatagcggca aaagcggcga cccaggtttt gatttcatgt gtcgactttc
    12061 cggcaatggc ggaaagctct tcgttactga cggcatccag ttcctgtatg cgtccctgct
    12121 ccagcaaagt catgaactgg ttatcccaaa tcgggttgag cggatgcaga gttctctgat
    12181 cctcaacaaa cttcacagcg gcgctaatca cccgttgctg acgcaattcg cgctcactgg
    12241 cgggtaaatc tttcccgctc cccaacagac ggtcgcgcat atgggcatcg gctttcgcca
    12301 gttcgggcac cggcggctga tgggaaagcc caccggaacc caggaacagc acgcgtttat
    12361 tgagagtgct ggtgaaacgt ccaatggctt cacccaacat gcgggtacgc tggaaaccgg
    12421 gcagcggcgt ggcgacaccg ttgatgaaca caggcagaac tggcacctta tccagcccac
    12481 cgagcaggaa ctccagcggc tgggagaacc cgtggtccac ctgcatacag taagaaacgg
    12541 caagatcgat cccgctcttc atgacggcat gcgcacaggc ctccgccagc tccacaggca
    12601 cgggcagctc tcctgccgca ctgccgaaat caccaattgc cgtcgctcca acgcctaaac
    12661 agaacggtgg catcacgtca tagaaaaagc cgttgtagtg atctggcgca aacagcacca
    12721 ccagttcagg ggagaaagcc gcaatacgct cgcgggcgct ggcaatcacg ccattgacct
    12781 catcgagtac ctcttgtgcc gggtcgacat accccaccag cggcgagtgg gaaagacaat
    12841 gaagataagc gtgcatatca ggctaccttt tcgacagaaa cgtcggcatc agggcgggtc
    12901 agcgtcatca ccgacgccag tttattcagg gtcttgccca gggtttgcgg aatggcggtg
    12961 gcggcaacaa agcgatccgg gcgcatcacc accagcgaag cattgtgttg tgcgaaccag
    13021 ctacgcagac gaccttgcgt atcgcccacg cgtagcacgc cgtcgtggtt atcctgtgcc
    13081 gtatgaattt gcacttccgg caccacctga ataaagcgtg tgcccaacgc gcgccactgc
    13141 tggatttgct cgtcgctcat cccccacagt ggattgcatc cccagccaat taccgcgaag
    13201 ttcgcgccga tcgcgttatc gagcagcgtc acgtcgccgt tttccagcgt gactttcggc
    13261 tgaataaaca tcttgccgac cggagagtgc ttcgcttcgc cctcacgtac cagcgcaccg
    13321 ccgtaatatt gcggcatcgg cttgaagcgc atttcgagga agtagcgttt tactggcggc
    13381 agataattca gcagccagga aacgccgtca cgtaacgtac cctgccagcg tttcggcgga
    13441 gccagcacgt tgcccgccgt cacggacagg tcaatcatcg ctttggcgtg atcgcgacgt
    13501 tcttgttgat aggtattgag cagcgcatcg cgggctttcc cctggataac caacgccagt
    13561 ttccaggcga ggttaaaggc gtcgcgcata ccactgttat agccctgccc ctgccacacc
    13621 ggcatgatgt gcgcggcatc gcccgccagc agtacgcgat caatacggaa acgttgcgcc
    13681 agtcgcgcgt tatgggtgta gacacgctgg cgaatcaatt caacattgtc cggattaggc
    13741 agcactttgc ttaacagctt gcgcatattt tgcggctcac gcagctgctc ttcggtttct
    13801 cccggcatca ccataaattc aaagcgacgt accgcatgag gcagcgcggc agaaacatac
    13861 gggcgcaccg gatcgcaaca caaatagata tgcggcgtac ttaacggatc gttggcgata
    13921 tctaccacaa tccactgatt tggcgcagtt ttaccttcaa acggcacatt cagggtgcga
    13981 cgaacaaaac ttgccccacc atcacaggcc accagccact gggctttgat cgtttcccgc
    14041 tgcccttctg ccgttttcag gtgcaaggtc acttcgtcat cttgctggct gaaggcctcc
    14101 agctcgcggg aaaacaagca gcgcacattc ggaaaacgcg acaacccttc cagcatcacc
    14161 gcatcgacct gtggctgaat aaaggcgtta cggcgcggcc agccaaattc atcggtcatt
    14221 ggctgaatat cagcaaaaca gcggcctttt ggggtgagaa aacgcatcgc gtgccacggt
    14281 gtagtgtgcg gcagaacatt ctcgaccagg ccgaccgact gcatggtgcg cagcgcctcg
    14341 tcatcaatac caatcgcacg cgggtagtcg atcaacttat cgagtttctc caccaccagc
    14401 acgtcaatgc ccatctgacc gagatagttc gccatcatca gcccgaccgg accggcacca
    14461 gagatcgcca cctgaacgct atggttaaca gcaggctgga tgtcagggtg ttgtattgcc
    14521 atttcagtac ctcacgactc ggacaaaatg ccgttgcgcg cacagtacgg cgcaacttat
    14581 tttgttaaaa acatgtaaat gattttttat tgtgcgctca gtataggaag ggtgttttcg
    14641 gctacaatca aaacatgccc gaatgtgcac caggtgcaca acgttgtttt aactatagaa
    14701 atgtcaatta atatgcagaa caatgagcag acggaataca aaaccgtgcg cggcttaacc
    14761 cgcggtctaa tgttattaaa tatgttaaat aaacttgatg gcggtgccag cgtcgggctg
    14821 ctggcggaac tcagcggcct gcatcgcacc actgtgcggc gactgctgga gacgctgcag
    14881 gaagagggat atgtccgccg tagcccctcc gatgacagtt ttcgtctgac catcaaagtg
    14941 cggcaattaa gcgaaggatt tcgtgacgaa cagtggattt ctgcactggc ggccccgctg
    15001 ctgggcgatc tgttgcgcga agtggtatgg ccaacagatg tgtccaccct ggatgttgat
    15061 gcaatggtgg tacgcgaaac cacccaccgt ttcagccgct tatcctttca ccgggcaatg
    15121 gtcgggcgac gtttaccgct cctgaaaacc gcctcgggcc tgacctggct ggccttttgc
    15181 ccggaacaag aacgcaagga attaatcgaa atgttagccg cccgccccgg tgatgactat
    15241 caactggcac gagaaccgtt aaagctgcaa gccattctgg cgcgcgcgcg caaagagggt
    15301 tacggacaga actaccgcgg ctgggatcag gaggagaaga tcgcctctat cgccgtaccg
    15361 ctgcgcagtg aacaacgggt gattggctgt ctgaatctgg tgtatatggc gagcgcaatg
    15421 accattgaac aggccgcgga aaagcatctt ccggcgctac aacgggtagc aaaacagatc
    15481 gaagaagggg ttgaatcgca ggctattctg gtggccggac ggcgaagcgg cgtgcattta
    15541 cattgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
    15601 gagtcaattc agggtggtga atgtgaaacc agtaacgcta tacgatgtcg cagagtatgc
    15661 cggagtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
    15721 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
    15781 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
    15841 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
    15901 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
    15961 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
    16021 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
    16081 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
    16141 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
    16201 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
    16261 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
    16321 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
    16381 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
    16441 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
    16501 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg tccaggcggt
    16561 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
    16621 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaagt
    16681 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagtcag ctcactcatt
    16741 aggcacccca ggctttacac tttatgcgtc cggctcgtat gttgtgtgaa attgtgagcg
    16801 aataacaatt tcacacagga aacagctatg accatgatta cggattcact ggccgtcgta
    16861 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    16921 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    16981 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggttccg
    17041 gaaagctggc tggagtgcga tcttcctgtc gccgatactg tcgtcgtccc ctcaaactgg
    17101 cagatgcacg gttacgacgc gcccatctac accaacgtga cctatcccat tacggtcaat
    17161 ccgccatttg ttcccacgga gaatccgact ggttgttact cgctcacatt taatgttgat
    17221 gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa ctcggcgttt
    17281 catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagccgttt gccgtctgaa
    17341 tttgacctga gcacattttt acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgc
    17401 tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg cattttccgt
    17461 gacgtctcgt tgctgcacaa accgagcaca caaatcagcg atttccatgt tgccactcac
    17521 tttaatgatg atttcagccg cgcggtactg gaagcagaag ttcagatgta tggcgagctg
    17581 cgcgatgagc tgcgggtgac ggtttctttg tggcagggtg aaacgcaggt cgccagcggc
    17641 accgcgcctt tcggcggtga aattatcgat gagcgtggtg gttatgccga tcgcgtcaca
    17701 ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa tctctatcgt
    17761 gcggtggttg aactgcacac cgccgacggc acgctgattg aagcagaagc cagcgatgtc
    17821 ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg
    17881 attcgcggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat ggatgagcag
    17941 acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt gcgctgttcg
    18001 cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct gtatgtggta
    18061 gatgaagcca acattgaaac ccacggcatg gtgccaatga atcgtctgac cgatgatccg
    18121 cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac
    18181 ccgagtgtga tcatctggtc gctgggaaat gagtcaggcc acggcgctaa tcacgacgcg
    18241 ctgtatcgct ggatcaaatc tgtcgatcca tcccgcccgg tgcagtatga aggcggcgga
    18301 gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag
    18361 cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc tggagagacg
    18421 cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg cggcttcgct
    18481 aaatactggc aggcgtttcg tcagtacccc cgtttacagg gcggcttcgt ctgggactgg
    18541 gtggatcagt cgctgattaa atatgatgaa aatggcaatc cgtggtcggc ttacggcggt
    18601 gattttggcg atacgccgaa cgatcgccag ttctgcatga acggtctggt ctttgccgac
    18661 cgcacgccgc atccagcgct gacggaagca aaacaccagc aacagttttt ccagttccgt
    18721 ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag cgataacgag
    18781 ctcctgcact ggatggtggc gctggatggc aagccgctgg caagcggtga agtgcctctg
    18841 gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca gccggagagc
    18901 gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa
    18961 gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc
    19021 ccctccgcgt cccatatcat cccgcaacta accaccagcg aaacggattt ttgcatcgag
    19081 ctgggtaata agcgttggca atttaaccgt cagtcaggcc ttctttcaca gatgtggatt
    19141 ggcgatgaaa aacaactgct gacgccgctg cgcgatcagt tcacccgcgc gccgctggat
    19201 aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt cgaacgctgg
    19261 aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgcacggc agatacactt
    19321 gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa aaccttattt
    19381 atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac cgttgatgtt
    19441 gaagtggcga gcgatacacc gcatccggcg cggattggcc tgaactgcca gctggcgcag
    19501 gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc cgaccgcctt
    19561 actgccgcct gttttgaccg ctgggatctg ccattgtcag acatgtatac cccgtacgtc
    19621 ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg cccacaccag
    19681 tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact gatggaaacc
    19741 agccatcgcc atctgctgca cgcagaagaa ggcacatggc tgaatatcga cggtttccat
    19801 atggggattg gtggcgacga ctcctggagc ccgtcagtgt cggcggaatt ccagctgagc
    19861 gccggtcgct accattacca gttggtctgg tgtcaaaaat aataataacc gggcaggcca
    19921 tgtctgcccg tatttcgcgt aaggaaatcc attatgtact atttaaaaaa cacaaacttt
    19981 tggatgttcg gtttattctt tttcttttac ttttttatca tgggagccta cttcccgttt
    20041 ttcccgattt ggctacatga catcaaccat atcagcaaaa gtgatacggg tattattttt
    20101 gccgctattt ctctgttctc gctattattc caaccgctgt ttggtctgct ttctgacaaa
    20161 ctcgggctgc gcaaatacct gctgtggatt attaccggca tgttagtgat gtttgcgccg
    20221 ttctttattt ttatcttcgg gccactgtta caatacaaca ttttagtagg atcgattgtt
    20281 ggtggtattt atctaggctt ttgttttaac gccggtgcgc cagcagtaga ggcatttatt
    20341 gagaaagtca gccgtcgcag taatttcgaa tttggtcgcg cgcggatgtt tggctgtgtt
    20401 ggctgggcgc tgtgtgcctc gattgtcggc atcatgttca ccatcaataa tcagtttgtt
    20461 ttctggctgg gctctggctg tgcactcatc ctcgccgttt tactcttttt cgccaaaacg
    20521 gatgcgccct cttctgccac ggttgccaat gcggtaggtg ccaaccattc ggcatttagc
    20581 cttaagctgg cactggaact gttcagacag ccaaaactgt ggtttttgtc actgtatgtt
    20641 attggcgttt cctgcaccta cgatgttttt gaccaacagt ttgctaattt ctttacttcg
    20701 ttctttgcta ccggtgaaca gggtacgcgg gtatttggct acgtaacgac aatgggcgaa
    20761 ttacttaacg cctcgattat gttctttgcg ccactgatca ttaatcgcat cggtgggaaa
    20821 aacgccctgc tgctggctgg cactattatg tctgtacgta ttattggctc atcgttcgcc
    20881 acctcagcgc tggaagtggt tattctgaaa acgctgcata tgtttgaagt accgttcctg
    20941 ctggtgggct gctttaaata tattaccagc cagtttgaag tgcgtttttc agcgacgatt
    21001 tatctggtct gtttctgctt ctttaagcaa ctggcgatga tttttatgtc tgtactggcg
    21061 ggcaatatgt atgaaagcat cggtttccag ggcgcttatc tggtgctggg tctggtggcg
    21121 ctgggcttca ccttaatttc cgtgttcacg cttagcggcc ccggcccgct ttccctgctg
    21181 cgtcgtcagg tgaatgaagt cgcttaagca atcaatgtcg gatgcggcgc gagcgcctta
    21241 tccgaccaac atatcataac ggagtgatcg cattgaacat gccaatgacc gaaagaataa
    21301 gagcaggcaa gctatttacc gatatgtgcg aaggcttacc ggaaaaaaga cttcgtggga
    21361 aaacgttaat gtatgagttt aatcactcgc atccatcaga agttgaaaaa agagaaagcc
    21421 tgattaaaga aatgtttgcc acggtagggg aaaacgcctg ggtagaaccg cctgtctatt
    21481 tctcttacgg ttccaacatc catataggcc gcaattttta tgcaaatttc aatttaacca
    21541 ttgtcgatga ctacacggta acaatcggtg ataacgtact gattgcaccc aacgttactc
    21601 tttccgttac gggacaccct gtacaccatg aattgagaaa aaacggcgag atgtactctt
    21661 ttccgataac gattggcaat aacgtctgga tcggaagtca tgtggttatt aatccaggcg
    21721 tcaccatcgg ggataattct gttattggcg cgggtagtat cgtcacaaaa gacattccac
    21781 caaacgtcgt ggcggctggc gttccttgtc gggttattcg cgaaataaac gaccgggata
    21841 agcactatta tttcaaagat tataaagttg aatcgtcagt ttaaattata aaaattgcct
    21901 gatacgctgc gcttatcagg cctacaagtt cagcgatcta cattagccgc atccggcatg
    21961 aacaaagcgc aggaacaagc gtcgcatcat gcctctttga cccacagctg cggaaaacgt
    22021 actggtgcaa aacgcagggt tatgatcatc agcccaacga cgcacagcgc atgaaatgcc
    22081 cagtccatca ggtaattgcc gctgatacta cgcagcacgc cagaaaacca cggggcaagc
    22141 ccggcgatga taaaaccgat tccctgcata aacgccacca gcttgccagc aatagccggt
    22201 tgcacagagt gatcgagcgc cagcagcaaa cagagcggaa acgcgccgcc cagacctaac
    22261 ccacacacca tcgcccacaa taccggcaat tgcagcggca accagataaa gccgcagaac
    22321 cccaccagtt gtaacaccag cgccagcatt aacagtttgc gccgatcctg atggcgagcc
    22381 atagcaggca tcagcaaagc tcctgcggct tgcccaagcg tcatcaatgc cagtaaggaa
    22441 ccgctgtact gcgcgctggc accaatctca atatagaaag cgggtaacca ggcaatcagg
    22501 ctggcgtaac cgccgttaat cagaccgaag taaacaccca gcgtccacgc gcggggagtg
    22561 aataccacgc gagccggagt ggttgttgtc ttgtgggaag aggcgacctc gcgggcgctt
    22621 tgccaccacc aggcaaagag cgcaacaacg gcaggcagcg cccaccaggc gagtgtttga
    22681 taccaggttt cgctatgttg aactaaccag ggcgttatgg cggcaccaag tccaccgccg
    22741 cccatcagag ccgcagacca cagccccatc accagtggcg tgcgttgctg aaaccgccgt
    22801 ttaatcactg aaggcatcac cgcctgaatg ataccgatcc ccaccccacc aagcagtgcg
    22861 ctgctaagca gcagcacact ttgggggtaa agctcacgca tcaatgcacc gacggcaatc
    22921 agcaacagac tgatggcgac gctgcgacgt tcgctgacat gctgatgaag ccagcttccg
    22981 gccagcgcca gcccgcccat ggtgaccacc ggcagagcgg tcaacagggc agccacgcta
    23041 aagctcattc cgctcgcctg gcgcaattgc ggtagcagtg gaccgacgga ggtgagcagt
    23101 ggtcgcatat taagaccaat cagcaccaga accagcagca tcgtgaacgt cttttgatgg
    23161 ttgcgcatgg ctgtcagaac ggtttggtcg gcagatattt accatctaag gtgatgaccg
    23221 cacgttcgcc accttccggg tccgccactt tcttaacgtc gagtttgaag ttaatcgcgc
    23281 tgataatgcc atcgccaaac ttctcatgaa ccaacgcttt cagggttgtg ccgtacacct
    23341 gcaacatttc atagaaacga tacatcgttg ggtcggttgg aatacggtca tcaatgcagc
    23401 cacgcagtgg aatcatctgc aacagtagaa tggcgtcttc gtcgagatcc agcttcgccc
    23461 cgaccaggcg ggcggcgtcg gcaggaagcg cctgctgccc cagcaaagcc gcggttacga
    23521 aggcttctgc cagaccggta ccgtcggcaa tctcggcaaa tgagagatct tttttagctt
    23581 tgctgagcaa aatggcatcg gcaagatcaa gacgaatatt gcggttaatt tgtgactgaa
    23641 tcatggtgga tctcctgatg gtttaaaaat aaggttacgc tgcggtcggt tggcgtagcg
    23701 gtatggcaca aacgcgagga ttagcggcca gtggcacaaa ctggcgggtt gcgccgtcaa
    23761 aagctgcgat gctgccgctt tcaatgtcgt agacccagcc gtgcagggcg atccgcccct
    23821 cttcgagcgc caggcgcacc gatggatgag tttgcaaatt agccaactga gcaatgacgt
    23881 tttcacgtac catcgccgca gcttttgacg gtaaatcgga atgcgggcgc gcctcattaa
    23941 cgacgcgggc tgaatcggca taacgcagcc agtgggagac ggcaggcata tggtccatac
    24001 actgacagct ggcaatggcg gtcatcgcgc cacagttgga atgaccacaa atcacaatgt
    24061 cagatacccg aagcgcagcg acggcatact ccaccgaagc agaaacgcca ccgggttccg
    24121 gcccgtagga agggacaata ttacctgcgt tgcgaataac gaacagatcg ccaggctcac
    24181 gttgcgtcac cagctcaggg accagacggc tgtcggagca ggagataaaa agggtgcgcg
    24241 ggctttgctg tgtcgccagc tgtttaaata aggcttcccg cttcggaaat gcctcgcgct
    24301 ggaatttaag gaatccatca ataatctctt tcaccgttaa cctctgtctg tctctggaat
    24361 gagaggcaga ctactcgccg attgtcataa ggtaaaagtc tcatttatga tgagttctat
    24421 tggatttact tataggttgg cgaatgctct ctcgacatat caactatttt cttgccgtgg
    24481 ctgaacatgg cagctttacc cgtgccgcca gtgcgttgca cgtctcccag cctgcgcttt
    24541 cccagcagat tcgccagtta gaggagagtt taggcgtgcc gctgtttgac cgtagcgggc
    24601 gaacgattcg tctcactgat gcaggagaag tctggcgaca gtacgccagc cgggcgttac
    24661 aggaactggg agcgggtaaa cgggcgattc atgatgttgc cgatctgacg cgaggatcgc
    24721 tgcgtatcgc cgtcaccccc acctttacaa gctactttat cggcccttta atggcggatt
    24781 tctatgcgcg ctaccccggc atcacgctcc agctacagga aatgtcgcag gagaaaatcg
    24841 aagatctgct ttgtcgcgac gagctggacg ttgggattgc cttcgcgcct gtgcattcgc
    24901 cggagctgga ggcaattcct ttactgacag aaagtttagc gttggtggtg gcgaaacatc
    24961 atccgctggc tgcctgtgaa caggtggcgt tgagtcgctt gcatgatgaa aaactggtcc
    25021 tgctcagcgc ggaatttgcc accagagagc aaattgacca ctactgcgag aaagcggggc
    25081 tacatccaca ggtggtcatt gaggcgaact caattagcgc ggttctggag ctgattcgcc
    25141 gcacttccct ttccacattg ttaccagcag cgattgccac acaacatgac gggcttaaag
    25201 cgatttctct tgccccgcca ctactggaga gaacggcggt tttgttgcgg cggaaaaata
    25261 gctggcagac agccgccgcg aaggcatttt tgcacatggc gttggataaa tgcgcggttg
    25321 ttggcggaaa tgaatcgcgg tagccgctga cacaggtctg cggctggaat tgcctgatgc
    25381 gctacgctta tcaggcctac gagttcagtg ctctacgtag gccggataag gcgttcacgc
    25441 cgcatccggc ataaactaca gctggcagac tgccgctgcg aaggcatttt tgcacatggc
    25501 gttggaggaa tgcgcggatg ttggcggaaa tgaatcacgg tagccgctga cacagatcag
    25561 cggctggaat tgcctgatgc gctacgctta tcaggcctac gagttcagtg ctctacgtag
    25621 gccggataag gcgttcacgc cgcatccggc ataaactacg ctcgctgtaa cccagccgat
    25681 taccgtttgt aatcgatggc ttctggctgc tccagataca ccgttgtttg tgctggttgt
    25741 gtactggcaa tcaccttgcc gccacgtacc gaataacgta ccggaacctg acggcgcagc
    25801 gcatcaaacc cattttcagc cggcaggata atcaggttgg cgctgtttcc ggcggcaatg
    25861 ccgtaatcct gcaaattcaa cgtccttgcg ctgtggtggg tgattaaatt caggccatcg
    25921 ttaatctgcc cgtagcccat caactggcaa acatgcagcc ccatatgcag cacttgcagc
    25981 atattcgccg ttcccagcgg ataccacgga tcgaagacat catcgtgacc aaagcagacg
    26041 ttaatgccgg actccagcat ctctttaacg cgcgtgatgc cgcgacgttt tggatacgta
    26101 tcgaaacgtc cttgcagatg aatattgacc agcgggttgg cgacaaagtt aataccggac
    26161 attttcagca agcggaacag gcgtgaggta tacgccccgt tataggagtg cattgccgtg
    26221 gtgtggctgg cggtgactcg cgcgcccatg ccttcacggt gcgccagggc agcaacggtt
    26281 tcgacaaagc gcgactgctc gtcatcgatc tcatcacagt gaacgtcgat gagacggtcg
    26341 tatttttgcg ccagggcgaa ggttttatgc agcgactcca cgccgtattc acgggtaaat
    26401 tcaaaatgcg gaatcgcccc cactacatct gcccctaagc gtaacgcctc ttccagcaac
    26461 gcttcaccgt tgggatacga caaaatccct tcctgcggga aggcgacgat ttgcagatca
    26521 atccacggcg cgacttcctg cttcacttcc agcattgctt tcagcgcagt cagcgttgca
    26581 tccgaaacat cgacatgggt acgcacatgc tgaatgccgt tggcaatctg ccatttcagc
    26641 gtttgccagg cgcgttgttt cacatcgtca tgggttaata acgctttgcg ttcggcccag
    26701 cgttcaatgc cttcaaacag cgtgccggac tgattccagt tcggttgtcc ggcggtttgc
    26761 gtggtgtcca ggtgaatatg cggctccaca aacggcggta taactaaacc ttgttcggca
    26821 tccaggctgt tttcagttat gggcatcacg ccggattgcg catcaatggc gctgattttt
    26881 ccgtcctgca gatgaatctg ccacagcccc tcttcgcctg gtaaccgggc gttaataatt
    26941 gtttgtaaag cgttattcga cactgttagc ctccacatgc gtcattgctg ctgtcgtttt
    27001 acgattcaaa atcgggttaa ggatcagata gctcagcgcg ccacctaata ccgcgttgac
    27061 cggaacaatt cccggtaacc agtggcctgc agcaatcccc aaggcgaccg ccagaatcgc
    27121 cacccaattg acactcatca tacgcgtggt cgcaaagtgc tcatagcggc gacggttcat
    27181 cagatagtcg gcgatgatca cgccacccac tggaggaata gctgccgaaa ggaaggtcaa
    27241 ccagccgaca aaattgttat acagccataa tgcgcagacc gtaccgataa taccgttgat
    27301 taccgaaagg gttttgctcg acatcccggt aatgttggcg aaacctaaac ccgacgcata
    27361 gagtgcgtta tcgttggtgg tccagatatt cagccccagc accacaatcg caggcagcag
    27421 caggccctga gcaatcatca catcagagat atccgccatg cccagtgccg cagcgcccgc
    27481 tgcaccgaaa ataaacatca acgagttgcc gaggaaaaag gccaccatcg ccaccagcac
    27541 cgccagtttg gcattgcgac caaaccggac aaagtcagcg gtgagcgtac ccgcactgat
    27601 aaatgacccc acaaccagcg ccagcgcgac attgaaatct aacggttgtg cgggaacgac
    27661 cgcttttaat gcgtccaggc cgcccatgcc gttaacagcc agccacacgg aataaccgcc
    27721 caggcaggcg atagccggaa ccgcaatcac cgaaagaacc gtcagcgccg aaatgccaaa
    27781 aaagacggtg acggtcatca gtaaaccgga aacggcaatc agcaaattaa tatccagccc
    27841 ggttgcctta cccaccggaa tggcaaacat cgccacaccg acgccaaacc agccaacctg
    27901 agttccgccc agtagcagtg aaggcagcca tgagccttta acaccaaacg agaagcgagc
    27961 aagaagatga gtggtcaggc cggtttttgc gccaatgtaa ccgagaaatg aagtgtaaat
    28021 accgaggaga agattaccga tgagaactgc gaggaagaaa tcatgatagc taagaccggt
    28081 tccgagagtg ccgccggtcc acatactggc ggaaaagaag gttaatccca gcatgacgaa
    28141 cgtcaatgcc aatacccctt tccgcgccga ctgcgggact ggcccctggc taaagttgtt
    28201 atcttgcgac acgaaattcc ccctttttgc tgtttgaaac ccaaaaaatc cgccgcattc
    28261 tattcatctg aagataaaaa gcaatcgttt tcgtggggaa atatattttt tatatgaatg
    28321 agtgcaaaaa ggtgggggaa aaattatcgg tgtaaaaaca gtgggtagtg
//
LOCUS       DAZGAN020000055        27478 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGAN020000055 DAZGAN020000000
VERSION     DAZGAN020000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.055"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..204
                     /locus_tag="ACHKYO_RS13265"
                     /old_locus_tag="pgaptmp_002653"
     CDS             <1..204
                     /locus_tag="ACHKYO_RS13265"
                     /old_locus_tag="pgaptmp_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HGU4429479.1"
                     /translation="YLAYMEANGYRNVLSLKMFGLGLPVMLKEYGLNYEKRHTKQGIQ
                     TNLTLKEESYGDWLPKCDDPATA"
     gene            409..744
                     /locus_tag="ACHKYO_RS13270"
                     /old_locus_tag="pgaptmp_002654"
     CDS             409..744
                     /locus_tag="ACHKYO_RS13270"
                     /old_locus_tag="pgaptmp_002654"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429480.1"
                     /translation="MSHIGRMPEVKNRMFTLHPLFTTYHSEIKGENRKVNSVNSSFEK
                     KFFMWIKDALVWIRATDPNLLIKNDLTFRFIDGLKIDWSEKQWGHKRGHISLLCFIIC
                     FLSLTYFDV"
     gene            960..2225
                     /locus_tag="ACHKYO_RS13275"
                     /old_locus_tag="pgaptmp_002655"
     CDS             960..2225
                     /locus_tag="ACHKYO_RS13275"
                     /old_locus_tag="pgaptmp_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4429481.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLVDARGRRDEARKLLANGVDPSENKKAVKVEHEQEVITFEVV
                     AREWHASNQKWSASHSARVLKSLEDNLFAAIGKRNIADLKTRDLLVPIKAVESSGRLE
                     VAARLQQHTTAIIRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLQRIY
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFDTAMWTIPGEREPLEGVKHSQRG
                     SKMRTPHLVPLSRQALTILEKIKSMSGNRELIFVGDHDPRKPMSENTLNKALRVMGYD
                     TKTEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERMLM
                     LQWWADYLDANRMAAITPFDYAKINSSFR"
     gene            complement(2426..3895)
                     /locus_tag="ACHKYO_RS13280"
                     /old_locus_tag="pgaptmp_002656"
     CDS             complement(2426..3895)
                     /locus_tag="ACHKYO_RS13280"
                     /old_locus_tag="pgaptmp_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HGU4429482.1"
                     /translation="MNIKSAHFLPGTNKRLGDKYLLLECLGDGSHGWVWRAERLKDGK
                     IVAVKIPKDITREDRQLAEGKELLNVEPHENIVQIFDMGRIDNEWFYIEMEYFPSQTL
                     AQKLDDRQRNFGQTYERLFRIYRQVLCAVHYLAELPVPISHGDIKPHNILVGERDLVK
                     LTDFGSSALPEEIYVRTRENGGTVLYSAPEFSNVDSRRGSLEELLLGDIYSLGVLLYQ
                     LLTGKLPHDTPAQVQRHAPFKLPTEINSSIHTDLEQVVLTCLQKRAEDRFSTISDLIH
                     AFDSAATKQLKVGAIAPVFQTKPAQDWSSAVLEAMSDQSYQKAAQLASQEYSRSKDLQ
                     ALHQQLIALYRANRLFDFEKVVEDNKAILLEGKLTQPAALFELIVKANLQLRNISQAN
                     FWLLQRKQAEAENAATMYLESSILGLEAKYSEARVLLEKVNQTTPMKFHVLSQLILVC
                     EQMRDYSGAAAYLKAALRVAPLDNSMKEKKELYAKLGVI"
     gene            complement(3892..4434)
                     /locus_tag="ACHKYO_RS13285"
                     /old_locus_tag="pgaptmp_002657"
     CDS             complement(3892..4434)
                     /locus_tag="ACHKYO_RS13285"
                     /old_locus_tag="pgaptmp_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429483.1"
                     /translation="MPEVAWVNETFPLLASGKLLDQDVLVHSLGCSVWNTFGHEQSFM
                     AVVECPAPGTFGADIRSDSGWFHKSSASPVCLIEFERFDGSAKGQQKLEEKLKNLLEA
                     AQRWNHCPKTLVLSAWSQGLVGVPDTQKLKDICRMGFTSSTGTQVIAAPDVEVVFSRF
                     LFIKNLNMIVLDRIHYEVLM"
     gene            complement(4442..6127)
                     /locus_tag="ACHKYO_RS13290"
                     /old_locus_tag="pgaptmp_002658"
     CDS             complement(4442..6127)
                     /locus_tag="ACHKYO_RS13290"
                     /old_locus_tag="pgaptmp_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HGU4429484.1"
                     /translation="MAYKVLGKLVGNTGDASQLTMVVQDSFSVRRGEFVRIMHQERKD
                     EGQVAVLGRVTKISRTNMLYNAGFGDGVTELELLPGANVTGENMFAQVELVGYRDPVT
                     RQIKIPRRPLNPGTAVETVDFQFLSDFYEFNEHSSLHLGNLVGYDKGENTVPVFIDVN
                     KLVTEHLAVLAMTGSGKSYTVGRIIERLVAVNNGTVVVFDPHGEYGKALAGGNLQFSS
                     FLEATDDKRDQEALPLIKQTFEKLQAAGAGIQVYSPQHESFKHKYASKNTPLALQFDH
                     FEMDDIAEILPGLTEPQQRVLDVAIRYWRYADKTEPRDINRLRFLLGDGIEELKEWDD
                     LSEAEAKALSGRSAAVASMKLSRVLNEAKSFYSATMAEPTDIYKMVGRPSNQQGRLVV
                     VDLQGLSDTAKQVICALLSSEILKAASSKTDPLRPCFIIYEEGHNFAPAGGNAVSHRI
                     IKKIAGEGRKFGVGFGIVSQRPSKLDSDVTSQCNTLITMRLKNPDDQRFIAKASDMVS
                     KADLEELPSLSTGEALVCGRSIPAPLLVKVGSKALIHGGESPEVLRVWGSFSG"
     gene            complement(6117..7274)
                     /locus_tag="ACHKYO_RS13295"
                     /old_locus_tag="pgaptmp_002659"
     CDS             complement(6117..7274)
                     /locus_tag="ACHKYO_RS13295"
                     /old_locus_tag="pgaptmp_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429485.1"
                     /translation="MNENKTMNLAQSLAIDSQLRLPGSAGRLLKQVRDIEQVAPLFRD
                     AGLVKAVPKPTIVNLRVAGIATKSCLDKVLGGFIYASAAVRTDLLIDDNKVSTVGSDS
                     VSELDDIDFEAQRKRLDLIEIRQAYQLVEKKLLGYEPGDLILLDTPLFLARDMAPLER
                     NVRHKQEYEKTRQVIETFWQNHRSRIFPWNQNGVVLASILAERFSAIVSIAKQDLRTE
                     QGRKQILASDGFSKELMQNLEGLDESLVGVGDLRFINGILGNYSRTIAFRLSEQEKRI
                     EPNIEAKQGLIGFHFRSARGGQIKMVQLAGDEPEWKSADLDLVASRLMVLDMQNKGNA
                     MPLPQLLGYQQLGILPKFATFYRQGLHQALKNNDIEAGWLAGLDEGLNNGL"
     gene            complement(7277..8125)
                     /locus_tag="ACHKYO_RS13300"
                     /old_locus_tag="pgaptmp_002660"
     CDS             complement(7277..8125)
                     /locus_tag="ACHKYO_RS13300"
                     /old_locus_tag="pgaptmp_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429486.1"
                     /translation="MSRYLSAALASNRKGRFLQTVAGATPLMKDWISSPPASGLLIVQ
                     AEELTDANTMQHLYHWAMQAGCAALVINLKAEQFTLLAQLPYPLDWQLVPASLRGQEP
                     GLTALLASETDQAIAGFTGSADRYQHQAGDVVHTRYIRKHSNSGLLAFTTLPLWSLTL
                     LDHSELLVSWLNWFVDHAGIAERIIEPKAPSTDYTPDKHDLVVLLLLYAGGGMNLQAL
                     SEHNAVKLMFDVNSLDIVKRGEMLRQHDFIDDAGITATGKTCLQASQYWAYAPLLGEQ
                     LHTGTL"
     gene            complement(8136..12422)
                     /locus_tag="ACHKYO_RS13305"
                     /old_locus_tag="pgaptmp_002661"
     CDS             complement(8136..12422)
                     /locus_tag="ACHKYO_RS13305"
                     /old_locus_tag="pgaptmp_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429487.1"
                     /translation="MSFELNEQMVDSLAKQHRWAATGVSDYPPRDPLVGQSRFFKRFQ
                     TFLHTVDHDDDRFAHVFAVEAEWGRGKSRLGHELVAQINDCSRGWFVRDEQGQLHDKQ
                     LFNRDTQDKYLALYIRYSQVASDYQNSDNWFAFGLYQALLPLATQTFDGSIQSEIAKQ
                     AYERLMPFGFKSELLADALQLNANHSEEDLYTDQTLVVSLVQAAYKVLKSFGVQYMLV
                     VLDELETVAEAATFGLEDEQNKRLDGQAIRLIGKAIKEEDPRRKLPWLRYVALCSPLL
                     GQQLREIQSVARRFELVELEHNAFADVSDYVASLLKDGKLGFNYPAGLVEAAYAMSGA
                     NFGWFNVVMANVDAVLSQYQQAGRSIKDVGDLFEAVLAGSGRVARHVLDKGAIEGIQT
                     RDQALLTECRSLLYGQLPVRLAEMAYATELLALKNEDLEPIAAHYRKLSIDRLQCRQA
                     LEQAKFRRDKDEWYYPAVDQALNLDTLLDNLRTFSIKEQAASGDKGAVLLPLSKGEFK
                     YLLSLLYDHPAIEFAADALWHKLVGETTELDASEATHIGPSVAMLLRLDLRYRSAQQN
                     SLIFKDPAMGDAHEQAMSELSKQSSQKPLIKALARVTGFVRLLDNNWTYDENLLSNKA
                     GSDDAPLAILTEPRGRQGGLLTLDGFKLHPEGKALFAWVSNLIELNNLHTFAANHSHQ
                     NGRTPVLALTASAHLMEQYSRLDERNELRDAILLHYVNPSEADQLERIGLSLAACQLH
                     GVNLTADSFTAKFKNKLHALTTFATDAIHKWRQRLQQRGLIAWPLKVDGKLSPNDRDL
                     LFKGWYQLAIAHPELNGILDLQQQHGVPVNELSSLLDKLKVPGSYIAKGYTADEHAGL
                     FTELNNVQRSQAQIPLFLARIAHPNKKHKWQFEGFKQQFYFAYVAETSVTAKGVFNDW
                     MWWCGELNLLTLTNPTEKQAIWEHYPRSRLENAIREAQNWFRGNDMGSYAANVEVMSR
                     VYGYARINEMFAPLGKNKLGFVTVEAKEQLEKAQSLFNVLKQQEEQLADMVEANDTKV
                     LAGLIHKRAEVLELVAKVKPLNSSRPMLKDAHILSLEDKTTSLYQRIEQACLFAEFVE
                     CSAERINNRLVDLIIDVETECAPLTNFPKRLYTNTLRTIGHILDGALKDDTSSATGRK
                     EQQADSDTLLHYLRKLDLGRAHDKLSALAQEVGLNLHNDQQLPIAEIQGHILSSYRNC
                     KERFSKLVNNLTEQKLRAQQLQEWLSSATAEYQFADDIAELPKLVMKLQLIEDAIADL
                     PSDAESKRQSMQNSLRNGQFSSLRDLPEELLKPARGQLTPIQGQLLKIEERLNQVRRN
                     SIAQVNSWLPLLKPLLASQKQAEPAALTLEDVSNLGVRELQQVCESTQAKWQSQGEQI
                     LNDTGLTLADWQPIYKALSQNQEPVLTPEQQKGLVDKGIVKMRLTFATGL"
     gene            complement(12419..13705)
                     /locus_tag="ACHKYO_RS13310"
                     /old_locus_tag="pgaptmp_002662"
     CDS             complement(12419..13705)
                     /locus_tag="ACHKYO_RS13310"
                     /old_locus_tag="pgaptmp_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429488.1"
                     /translation="MDSITTRQNNDPVNSEAALNLCLQLWHQGGLTASKAALLLAAVP
                     ALRSLLQPIIQPKKKDAETDIISAFSLTAPLLDAFSDLSQSGEWQLALLGLNPDVRQH
                     WINLAAARCQEAGAMSDPMVLVKLIQQLGNASEWVLAQLENADFSPQIIASPLAQTER
                     DLLGHSLNDNAAIPALCRILRTSHTLFTVSEQNEPPASIQAVDVTAKQLTNNWCSGRL
                     LALPNTLLDEHNLKPNADWLLVSRSGHDNVPLTELFAQQPWLFLLSLIIFVQDAWAAE
                     QRGGLLLTLPAGQNAFAPGQINVAVQGIEGDEVSLGSLAEFLVLLLGELNIPLYPALD
                     ANTESINRLNRVLSSFIAELLAKKIWQFTEAGRGESGQYRIHTSFSDACYSLPLAPLF
                     GYKSQTLQRAIKQLAQNCYANKKRAANRINLQGSSL"
     gene            complement(13723..15249)
                     /locus_tag="ACHKYO_RS13315"
                     /old_locus_tag="pgaptmp_002663"
     CDS             complement(13723..15249)
                     /locus_tag="ACHKYO_RS13315"
                     /old_locus_tag="pgaptmp_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU4429489.1"
                     /translation="MDKFFRTNSSILVERLDPNFYQPSLVENDKLIKSKGWVELQSLY
                     EASGIGNTSAVERYYSEAADSIPYISGKVIKSFNIDLDECQRISLDSHKNELTKSALK
                     PTDVLVIRKGDMGNACVVPSEVNEANCSSEVIYLKMKASSDPYYLVSYLNCDQGQKAF
                     KRLGRGTIIPGVSLLDVPRLPIPKVSEFVQKYIGDKVRQAEQLRAWAKLLRTSVDAHL
                     NSLNLPINEPPALLNRVSAQTMEDRLDPRPYRTHYLCLVREIEKLPHDSISTLVELAS
                     GCPVSSNDFLENSGIPLVRIRNIGFDDFIGLDTGVSQDVYQDATKYQAKDKMIVVGMD
                     GIFRSQFFISDELPMLVNQRVAMLSPQNIRGELLTHWLNRPEGQMQLNQWAVKTTVEH
                     TSLSDIGRVLIPRLDKSLENKLADYLLNARLAYRYAKFLTQVAKTLVEALIEGQLTEQ
                     QLIQAQQALEDGDNSFDQAILSKLSAEGYAIEGATPLFSDVDELYSLLEEAAQAEAEE
                     "
     gene            complement(15249..17396)
                     /locus_tag="ACHKYO_RS13320"
                     /old_locus_tag="pgaptmp_002664"
     CDS             complement(15249..17396)
                     /locus_tag="ACHKYO_RS13320"
                     /old_locus_tag="pgaptmp_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGU4429490.1"
                     /translation="MAKEAKKQDPQQQLEALFNKLVKQHSEFTQPLVELPEHEFAKAG
                     LIQGLVEGNLTPPVMLIALTEGHWEYAADTAKFDDLAYQLLSDIEGDWPVYAVVDDGL
                     NHRILALFGADGSDGTHGADTLPGLEELRSFDRMERDPTFRWSMRVYTRLMQRFDAFH
                     ENVYRVTKDKVNDKNDIIEEVAKLLFLETFRLHHDEDLTFKDDEGNTLRFRDVFNWQY
                     VESHGDKAVAQIKAAFEEFKSHENYVVISDDGSRNPIFSKETHLRLSVAKNYQDLLEA
                     IQNLGPVKTNDGKIAKEHGTLADVSGDLLGRVFDVFLRANFESKGGLGVYLTPNPVKQ
                     AMLEIAMHDIDDDDEMRSRLANGDFRFCDPTCGSFGFGSVALSQIDKWIDFKLVLADD
                     KKESLKQKLRDCAFTGADAAPRMVMLARVNMALQGAPKAQIFYTDNSLTTNALKPNSF
                     DLICTNPPFGTPKFDKGKNGQNSKANYEANMEQVLGGFRPTKKVVDSYNAWYDHVKMK
                     WEDIGDLELDENGEPKWAGYRTDLRRTGGTDKKPFYSLQPTTAGLALGSKPDSKGNWQ
                     PVGATIDPAVLFIDRCLQLLKPGGRLLIVLPDGILCNSGDRYVREYIMGKKDEKTGEF
                     VGGKAIVKAVISLPSDCFKLSGTGAKTSILYLQKRHANPNQPEQFLPEPQTDVFMAVA
                     ETLGYVVKNNIEDYNAGVANDLDKIVSAYKRGE"
     gene            complement(17447..17833)
                     /locus_tag="ACHKYO_RS13325"
                     /old_locus_tag="pgaptmp_002665"
     CDS             complement(17447..17833)
                     /locus_tag="ACHKYO_RS13325"
                     /old_locus_tag="pgaptmp_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429491.1"
                     /translation="MTHEYQRQSLEQLITQQTATPRSAAVVFNENQTLWQTLGFSQSQ
                     VSLWLASLPQSNHLKGPTTATYQVIPDIAAHLVSLLHQAGGRMPLAQVLKKLPAGITT
                     SEQQIRKLAQKHAQIEIKGPLLVLIN"
     gene            complement(17830..19173)
                     /locus_tag="ACHKYO_RS13330"
                     /old_locus_tag="pgaptmp_002666"
     CDS             complement(17830..19173)
                     /locus_tag="ACHKYO_RS13330"
                     /old_locus_tag="pgaptmp_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HGU4429492.1"
                     /translation="MGEVISVFEYDLLGSGKAASVGAKPVPPQVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIQTQQATLQAQRMPLLEIFINQFLHSVSQLLKQGLRSDYVSKQGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDDYMPDCAANRLLHSALDKLLSLQLSSDNQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPDELPPHLKVLPQAAIYSLVKHGVKDCFKLRPDLLIQSNKPVQTE
                     MVMDTKWKLVNSSQQTKSLYGLAQADFYQMFAYGQKYLGGNGEMYLIYPAHDDFSQPI
                     PQHFAFSETLKLWVVPFRIMAKRGERMMWPNDVSCTMSPQLDRMAASVSYSGGMR"
     gene            complement(19166..21241)
                     /locus_tag="ACHKYO_RS13335"
                     /old_locus_tag="pgaptmp_002667"
                     /pseudo
     CDS             complement(19166..21241)
                     /locus_tag="ACHKYO_RS13335"
                     /old_locus_tag="pgaptmp_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
     gene            complement(21411..22283)
                     /locus_tag="ACHKYO_RS13340"
                     /old_locus_tag="pgaptmp_002668"
     CDS             complement(21411..22283)
                     /locus_tag="ACHKYO_RS13340"
                     /old_locus_tag="pgaptmp_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HGU4429493.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSHYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQQGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EVFVEWHRKEVFRG"
     gene            22383..22487
                     /locus_tag="ACHKYO_RS13345"
                     /old_locus_tag="pgaptmp_002669"
                     /pseudo
     CDS             22383..22487
                     /locus_tag="ACHKYO_RS13345"
                     /old_locus_tag="pgaptmp_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(22495..23475)
                     /gene="nanS"
                     /locus_tag="ACHKYO_RS13350"
                     /old_locus_tag="pgaptmp_002670"
     CDS             complement(22495..23475)
                     /gene="nanS"
                     /locus_tag="ACHKYO_RS13350"
                     /old_locus_tag="pgaptmp_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4429494.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPSIPDNAGVLIVPCCRGGSAFIAGSEGTYSERHGASHDACRWGSDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGVISDRFVEAILQFWREK"
     gene            complement(23540..24646)
                     /gene="nanM"
                     /locus_tag="ACHKYO_RS13355"
                     /old_locus_tag="pgaptmp_002671"
     CDS             complement(23540..24646)
                     /gene="nanM"
                     /locus_tag="ACHKYO_RS13355"
                     /old_locus_tag="pgaptmp_002671"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGU4429495.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAALVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            complement(24666..25382)
                     /gene="nanC"
                     /locus_tag="ACHKYO_RS13360"
                     /old_locus_tag="pgaptmp_002672"
     CDS             complement(24666..25382)
                     /gene="nanC"
                     /locus_tag="ACHKYO_RS13360"
                     /old_locus_tag="pgaptmp_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HGU4429496.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            26836..27438
                     /gene="fimB"
                     /locus_tag="ACHKYO_RS13365"
                     /old_locus_tag="pgaptmp_002673"
     CDS             26836..27438
                     /gene="fimB"
                     /locus_tag="ACHKYO_RS13365"
                     /old_locus_tag="pgaptmp_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HGU4429497.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT         7120 a         6400 c         6142 g         7816 t
ORIGIN      
        1 tatctggcct acatggaggc aaacggctac cggaatgtac tcagcctgaa aatgttcggg
       61 ctggggctgc cggtgatgct gaaagaatac gggctgaatt acgagaagcg ccataccaaa
      121 caggggatac agaccaacct gacgctgaaa gaggaaagct acggcgactg gctgccaaaa
      181 tgtgacgacc ctgcaacagc ctgacccacc tgaccggcat ctgccggtct ttttttatcc
      241 ctgaattccc cgaaggtgaa caatccactg ttcacccttc accgtatatt cacccgttat
      301 cacactgaaa ttaaaagaga aaaacgaaag gtgaacagtg tgaacaatca aatcaaaaaa
      361 aacttttttc ttcctgagtg atttcagtgc ggaggattaa tcaccggtat gagtcacatc
      421 ggcagaatgc cggaggtgaa gaatcgaatg ttcacccttc acccattatt caccacctat
      481 cactctgaaa taaaaggaga aaacagaaag gtgaacagtg tgaacagttc tttcgaaaaa
      541 aaatttttta tgtggataaa agatgcactg gtttggatcc gagctacgga tccaaactta
      601 ctgattaaaa atgatttaac tttccgattt attgatgggc tgaaaataga ttggtcagaa
      661 aaacagtggg ggcacaaaag ggggcatatt tcattgttgt gttttattat ttgtttttta
      721 tcattaactt attttgatgt ttgagtccgg ccttcgcacc aaaagtatgt aaatagacct
      781 caactgaggt ctttttttat gcctgaaatc cagtatccgc aaggctttcc tcgctttttc
      841 actcaaccta agtcaacctg attcaatcta catcaactta ctgatgcggg tacaactgcg
      901 ggtatttccc ggttcgatta tgtttgtacc cacacagggc ccttgaaagg atactcatca
      961 tggctctgag tgatgtgaag gtccgttcgg ctaagccgga agcaaaagcc tataaactta
     1021 ctgacggtga aggcatggtt ttgttggttc accctaatgg ctccaaatac tggcgacttc
     1081 gttatcgttt tggcggtaag gagaagatgc tggcgctggg gaaataccct gaagtctcgc
     1141 tggtcgatgc cagaggtcgc cgggatgaag cccgtaagtt gttagctaac ggcgtcgatc
     1201 ctagtgagaa caagaaagct gttaaggtag agcatgagca agaggtgata acatttgaag
     1261 tggtagccag agagtggcac gccagcaatc agaagtggtc tgcatcgcat agcgctcgtg
     1321 tattgaaaag cctcgaagat aatctctttg ctgctatcgg taagcggaac attgctgatc
     1381 tgaagactcg ggatctgctt gtccccatca aagcggtgga gtcgtctggg cgtctcgaag
     1441 tcgctgctcg tttgcagcag catactaccg cgattatacg ctttgctgta cagagtggat
     1501 taatcgacta caaccccgct caagagattg ctggtgcggt tgctacggcg aaaagacagc
     1561 atcgtgctgc tctggaactt aaccgcattc ctgaattgct tcaacgcatc tatcactatt
     1621 ctggcaggcc gttaacccga ctggcggttg aactcacatt gttagttttc atccgttcaa
     1681 gcgagttgcg ttttgctcgc tggtcagaag ttgattttga tactgccatg tggacgattc
     1741 cgggagaacg tgagccactg gagggagtta agcattctca gcgtggttcg aagatgcgga
     1801 ctccacatct tgtaccctta tctcgacaag ctctcaccat tctggaaaaa atcaaaagca
     1861 tgagtggaaa ccgtgagctg atcttcgttg gtgatcacga tccgcgtaag ccaatgagtg
     1921 agaatacgct taacaaggcc ttgcgagtga tgggctatga cacgaaaacg gaagtgtgtg
     1981 gacatggttt caggacgatg gcgtgtagtt ctttgattga atcgggattg tggtcgaggg
     2041 atgcagtaga gcggcagatg agtcatcagg aacgtagctc agtacgggcg gcttatatcc
     2101 ataaggcgga acatcttggg gagcgaatgt tgatgctgca gtggtgggct gattatctgg
     2161 atgcgaatag aatggcggct atcactccat ttgactatgc aaagattaat agttcttttc
     2221 gataattcta gctaagtata tgaatattaa ctttttttaa ttaaaggctt ataactgaga
     2281 agcctccgcc ctaatgggga gaggcttttg tcttaactca atcaagaagg aaatcgttat
     2341 gtaaaacttt atttttttgt ccggtatgtg aacggaagtg ttaaatatcc actatgcctt
     2401 gttcgcgcaa tggtcatcct ttcaatcaaa taactccaag ctttgcatac aattcttttt
     2461 tctctttcat gctgttatcc aatggtgcta ctcgcaatgc cgctttcaaa taagccgctg
     2521 caccagagta atcacgcatt tgctcgcaaa ctaaaatgag ttggcttaac acatggaact
     2581 tcattggagt tgtttggttt actttctcca atagtactct tgcttcagaa tatttcgctt
     2641 caaggccaag gatagatgat tctaaataca tggtggccgc gttttctgct tcagcttgct
     2701 ttcgttgcaa cagccagaaa tttgcttgac tgatattgcg taactgtagg tttgccttaa
     2761 ctatcaactc gaacaaagct gctggttgag tcaatttacc ctcaagtaag atcgctttat
     2821 tatcttctac aactttttca aagtcgaata agcgattggc gcgatacaag gcaataagtt
     2881 gctgatgtaa agcttgtaga tctttactgc ggctatactc ctgcgacgct aattgagcag
     2941 ctttttggta agattgatcg gacattgctt caagtaccgc acttgaccag tcctgagcag
     3001 gtttagtttg aaaaacggga gcaatagcac caacttttaa ttgcttagtg gcagcagaat
     3061 caaatgcatg aattaagtcg gaaatagtgg aaaagcgatc ctccgctcgt ttttgtaaac
     3121 aagtaagaac cacttgttcc aggtccgtat gaatagagct attaatctct gtcggaagct
     3181 tgaaaggtgc gtggcgctga acttgtgctg gcgtatcatg gggcagcttt cctgtcagca
     3241 gctggtatag caaaacacct aagctataga tgtcgcccaa tagtagctct tccaggctgc
     3301 ctctacggct atcgacgttg gagaactctg gggccgaata cagcacagtc ccgccatttt
     3361 cacgggtacg aacgtaaatt tcttctggta aagctgagct gccaaagtcc gtcaacttca
     3421 ccaggtcccg ctctccaacc aaaatgttat gtggcttaat atctccatga gatattggta
     3481 ctggtaattc ggccaaataa tgcacagcac agagcacttg acgatatatt ctgaacaacc
     3541 gttcataggt ctggccaaaa ttacgctgcc tgtcatcgag cttttgcgcc agggtttggc
     3601 tgggaaaata ctccatttca atatagaacc attcattatc tatacggccc atatcaaaaa
     3661 tttgcacaat attttcgtgc ggctcgacgt ttaaaagctc tttaccttcg gcaagctgac
     3721 ggtcttcccg agtaatatcc ttagggattt ttactgcgac aatttttcca tctttcaggc
     3781 gttccgcccg ccatacccag ccatggctgc catcaccaag gcattcaagt aacaggtatt
     3841 tgtcaccaag gcgtttattc gtccctggca aaaaatgtgc tgattttata ttcacatcaa
     3901 cacctcatag tgaattctgt ccaaaacaat catgttcagg tttttgataa ataaaaagcg
     3961 actaaatacc acctcaacat caggagcggc gattacctgg gtacccgtgg acgaagtaaa
     4021 acccatacgg catatgtctt tcagtttttg agtatcaggt acacctacta agccctggct
     4081 ccaggcactg aggaccaggg ttttggggca atgattccag cgctgtgctg cttccagtaa
     4141 gttttttaat ttttcttcca gcttttgctg ccccttcgct gaaccatcaa agcgttcaaa
     4201 ttcgattaaa cacacaggcg atgcagaaga tttatgaaac cagcccgagt cagagcgaat
     4261 atcagcacca aatgtccctg gagccgggca ttccactacc gccataaatg actgttcatg
     4321 cccgaatgta ttccaaacgc tacagcccag gctgtggacc aatacatcct ggtccaatag
     4381 cttaccgctt gctaaaagtg gaaatgtctc atttacccag gcaacttcag gcataggcac
     4441 cttagccact gaaagagccc caaacacgca gtacctctgg tgattcacca ccgtgaatta
     4501 atgccttact accaacttta acaagcagtg gtgcagggat tgaacgacca cacaccaaag
     4561 cttcaccggt tgataaactc gggagttcct ccagatcagc tttgctcacc atatctgatg
     4621 ctttagctat aaatcgttga tcgtcagggt tctttaagcg catcgtaatt agcgtattgc
     4681 actgagaggt cacatctgaa tctaatttcg atggacgctg gctcacaatg ccaaatccaa
     4741 caccaaattt acgaccttca ccagcgatct ttttaataat gcggtgactg actgcgttgc
     4801 cgccggccgg ggcaaaatta tgaccctctt cgtaaatgat aaagcatggg cgcagcgggt
     4861 ccgttttgct cgaggctgct tttaatattt cactagataa cagtgcacaa atgacttgct
     4921 tagcggtatc actcaaacct tgtaagtcaa caacgactaa acgcccctgt tgattgctcg
     4981 ggcgacccac cattttgtaa atatcagttg gctcagccat tgttgcgctg taaaaacttt
     5041 tagcttcatt aagcacacgt gaaagcttca tcgaggcgac ggcggcacta cggcctgata
     5101 acgccttcgc ctcagcttca gaaaggtcgt cccactcttt taactcttca ataccatcac
     5161 ctaataagaa gcgtaggcgg ttaatatctc gtggctcagt cttatctgca tagcgccaat
     5221 aacgaattgc aacatcgagt actcgttgct gcggttccgt taagccaggt aatatttcag
     5281 caatatcgtc catttcaaaa tggtcaaact gaagcgctaa tggcgtattc ttactggcgt
     5341 atttatgttt aaaagattca tgctgcggcg agtaaacctg aatacctgca ccggcggctt
     5401 gcagcttttc aaaggtctgt tttatcagtg gcagtgcttc ttgatcacgc ttatcgtcag
     5461 tggcttctaa gaagcttgag aattgcagat taccgccagc gagggcttta ccgtattcac
     5521 catgtgggtc aaatactact accgtaccgt tattaaccgc aactaaacgc tcaataatgc
     5581 ggccaactgt ataagactta cctgaacccg tcattgccag aacagccaaa tgctcagtga
     5641 ccaacttgtt cacgtcaata aaaacaggaa ctgtattctc acctttgtcg tagcctacta
     5701 agttacctaa gtgcaaactg gagtgttcat taaattcgta aaaatcactc aaaaactgaa
     5761 aatcgacggt ttcaacagcg gtacccgggt taagcgggcg acgtggaatt ttgatttgac
     5821 gagtaacagg atcgcggtaa ccaaccagct cgacctgagc aaacatattc tcaccagtta
     5881 cattcgcacc gggcaaaagc tcaagctcgg ttacaccatc gccaaagccg gcgttataga
     5941 gcatattagt acggcttatc ttggtcactc gccccagtac ggcgacttgc ccctcatctt
     6001 tgcgttcctg gtgcataatg cgtacaaact caccacggcg tactgaaaac gagtcttgta
     6061 caaccatagt taactgactg gcatcaccag tgttaccgac taatttacct aaaactttat
     6121 aagccattat tcaatccctc atcgaggcct gccaaccacc ctgcctcaat atcattattc
     6181 tttagtgcct ggtgtaaacc ctggcgataa aacgtagcaa atttaggtaa aataccaagc
     6241 tgttgataac caagtaattg tggcaacggc attgcattac ctttgttttg catatccagc
     6301 accatcaggc gagaggcaac taaatcgaga tcagcacttt tccattctgg ttcgtcaccc
     6361 gctaattgca ccatttttat ctgtccacct cgggcactac ggaagtgaaa accaattaga
     6421 ccttgcttag cttcaatatt gggttcaatg cgcttttctt gttcagacaa acgaaatgcg
     6481 atggtgcgcg aatagttgcc taaaatgcca ttgataaatc gcaaatctcc cacgccaacc
     6541 aaactttcat ccagtccttc cagattttgc atcaactctt tagaaaaccc atcagatgct
     6601 aaaatttgtt tgcgtccttg ttcggtacgt aaatcttgtt tggcgatact tacaatggca
     6661 ctaaagcgct cagccaaaat gctcgcaagt actacaccat tttgattcca gggaaaaatt
     6721 ctggagcgat ggttttgcca aaaagtttct atcacttgtc tggttttttc gtactcttgt
     6781 ttatgacgga catttctctc cagcggagcc atatcccgtg ctaaaaacaa cggcgtatcc
     6841 agcaaaatta aatcgccagg ctcataacct aaaagttttt tctcaactaa ctgataagcc
     6901 tgacgaattt caattaaatc aagccgttta cgctgagctt caaaatcaat atcatccagt
     6961 tctgataccg agtcgctacc caccgtgctg actttattgt catctattaa caaatctgtg
     7021 cgcactgctg cactggcata aataaaacca cccaatacct tgtctaagca cgatttagtc
     7081 gcaataccgg ctacgcgtaa attaacaatt gtgggcttgg ggaccgcttt cactaagcca
     7141 gcatcacgga atagcggcgc cacttgctca atatcacgga cctgtttaag caagcgtcct
     7201 gcacttcccg gtaagcgaag ctggctatcg atggcgagcg attgcgctaa attcatcgtt
     7261 ttgttctcat tcatgcttat agcgttcctg tgtgtagctg ctcgccaagt aatggggcat
     7321 aagcccaata ttgcgaggct tgtaggcatg ttttaccggt agctgttatg cccgcgtcgt
     7381 cgataaaatc gtgctgccgc aacatctcac cccgcttgac aatatcaagg ctattaacat
     7441 caaacatcag cttaaccgca ttatgctcag acagcgcttg taagttcatg ccaccgccag
     7501 cgtagagtaa tagcaataca accagatcgt gcttatctgg cgtgtagtca gttgatggcg
     7561 ctttaggttc gattattcgt tcagcaatgc ctgcatggtc cacaaaccaa ttcaaccagc
     7621 tcaccaggag ctcactatga tcgagtaaag tcagcgacca caaaggtaac gtggtaaagg
     7681 ctaatagccc actgttgctg tgttttcgta tgtagcgggt atgcactaca tcaccggcct
     7741 ggtgctggta gcgatctgcg ctgccagtaa agccagcaat agcctggtct gtttcactgg
     7801 ctagcaaagc ggttagcccc ggttcctgcc ccctcaagct cgctggtact aactgccaat
     7861 ccagtggata gggcaattgc gctaataagg taaattgttc tgctttcagg tttattacca
     7921 aagcggcaca acctgcctgc atagcccagt gatacaggtg ctgcatagta ttagcgtcag
     7981 tcaactcttc tgcttgcact attaacaaac cgctggctgg cggcgagcta atccaatcct
     8041 tcattaaggg ggtagccccc gcaacagttt gcaaaaaacg gcctttacga ttactggcca
     8101 gtgccgcaga taaataacga ctcatcgcgt cacctctaca aaccagtggc aaaggtaagg
     8161 cgcattttta caatgccttt gtctacaagt cctttctgtt gctcaggtgt taacacgggt
     8221 tcctgatttt ggctcaaagc cttataaatc ggttgccaat ccgccagtgt taagcctgta
     8281 tcatttaata tttgctcacc ttgactctgc catttggcct gggtgctttc acacacctgt
     8341 tgaagttctc tgacacccaa attgctcaca tcctccagag tcaacgctgc tggttccgct
     8401 tgcttttgag aagccaatag cggttttaat aacggcagcc agctgtttac ctgtgcgatg
     8461 gagttgcgtc ttacttggtt tagacgctct tcaattttaa gtagctggcc ttgaatcggt
     8521 gtaagttgac cgcgtgccgg ttttaaaagc tcttctggta aatcccgtaa cgatgaaaac
     8581 tgcccattgc gcaaactgtt ttgcatactt tgacgcttgc tctctgcatc actggggaga
     8641 tcggcaatag catcttcaat gagctgtaat ttcattacca gttttggtag ctctgcaatg
     8701 tcatcggcga actgatactc agccgtggcg ctactaagcc attcttgcag ttgttgtgca
     8761 cgtagttttt gctctgtgag gttattaacc agcttactaa atcgttcttt gcagttgcgg
     8821 tagctggaca aaatatgccc ctgaatctcc gctatcggca actgttgatc attgtgcagg
     8881 tttaaaccca cttcttgcgc taaggctgaa agcttgtcat gcgctctacc taaatcgagt
     8941 ttacgcaggt aatgcagtaa ggtatcgcta tctgcttgct gctcttttcg gccagtggca
     9001 ctgctggtat cgtcttttaa cgctccatct aaaatatgac ctatggtgcg cagcgtgttg
     9061 gtgtacagac gtttcggaaa attcgtcagc ggggcacact ctgtttcaac atctatgatt
     9121 aaatcaacga gtcggttgtt gatacgctca gcgctgcact caacaaactc ggcaaataaa
     9181 caggcctgtt caatccgttg ataaagcgag gtcgttttgt cttctaagct gagaatatgg
     9241 gcatctttta gcataggcct ggaactatta agcggtttaa cctttgctac cagctccagc
     9301 acttcagcgc gtttgtggat aagccctgcc aatactttcg tgtcgttggc ctcgaccata
     9361 tcagccaatt gttcttcttg ctgtttgagc acgttaaata gcgattgtgc tttttccagc
     9421 tgctctttag cttccacggt gacgaaaccc agcttatttt tacccagtgg cgcgaacatt
     9481 tcattaatgc gtgcataacc atacacgcgg ctcatgactt ccacattggc tgcatagctg
     9541 cccatatcgt taccacggaa ccagttttgc gcttctctaa tggcattttc taagcgggag
     9601 cgcgggtagt gctcccaaat tgcctgtttt tctgttggat tggttaaagt gagtaagttc
     9661 agctctccac accaccacat ccagtcatta aacacgcctt tggcggtaac actcgtttca
     9721 gccacatagg cgaaataaaa ctgttgctta aagccttcga attgccactt gtgcttttta
     9781 ttagggtgag caatacgggc taaaaataat gggatctgcg cctggcttcg ctgcacatta
     9841 ttaagttcag taaatagccc ggcatgttca tcggcagtgt aacctttggc aatatagctg
     9901 cccggcacct taagtttatc caacaagctg ctcagctcat tcactggcac gccgtgttgc
     9961 tgctgtaaat cgagaatgcc atttaactca gggtgagcaa tggccagctg ataccagcct
    10021 ttaaacaaca ggtctcggtc attcggtgat aatttaccat ctacctttaa cggccaggca
    10081 attaaaccgc gctgctgcaa acgctggcgc cacttgtgaa tggcatccgt ggcaaaggtt
    10141 gtcagggcat gcagcttgtt tttgaatttc gcggtaaagc tgtcggcagt taaattcacg
    10201 ccgtgcaact gacatgctgc caaacttaag ccaatacgct ctaactggtc tgcttcgctt
    10261 gggttgacat aatgcagcag tatcgcatca cgcaactcat ttctttcatc cagtcggctg
    10321 tattgctcca tgagatgtgc agaagcagtt aacgctaata ctggcgtgcg accattttgg
    10381 tggctgtggt ttgccgcaaa ggtgtgaaga ttgttcagct caattaggtt gctgacccag
    10441 gcaaacaagg ctttaccttc tgggtgaagc ttaaatccat ccagggtaag taaaccaccc
    10501 tggcggccac ggggctcggt taaaatggcc aaaggtgcat cgtcgctgcc cgctttatta
    10561 ctcagcaagt tttcatcata ggtccagttg ttatcaagta atctcacaaa gcccgttaca
    10621 cgggctaatg ccttgatgag cggcttctgg ctactttgtt tgctaagttc actcattgcc
    10681 tgttcatgag cgtcgcccat ggcgggatct ttaaaaatca gtgagttttg ctgggcactg
    10741 cgatagcgca aatccaggcg cagtaacatc gccacactgg ggccaatatg cgttgcttcg
    10801 ctggcgtcca gttcggtagt ttcacccact agcttgtgcc ataaggcatc ggcagcgaat
    10861 tcaatcgctg ggtggtcata cagcaagctg agtaagtatt taaactcacc ttttgacaga
    10921 ggtagtaata ccgcgccttt atcgccgctt gcggcttgtt ctttaattga aaaagtacgc
    10981 aggttatcca gcaaagtatc taagttcagc gcttgatcta ctgcagggta gtaccattca
    11041 tctttatcgc ggcggaattt ggcttgctcc agggcttgtc ggcactgtag gcggtcaatg
    11101 ctcagtttgc ggtaatgagc ggctatgggt tccagatctt cgtttttcag cgccagcaat
    11161 tctgtggcat aggccatttc agctaagcgc acgggtaatt ggccgtagag caaactacga
    11221 cattcggtca atagtgcctg gtcgcgagtt tgaatgcctt ctatcgcgcc tttatccagc
    11281 acatgcctgg ctacgcggcc agagcctgcc agcacagctt caaataaatc gcccacatcc
    11341 ttgatactgc ggcctgcttg ttggtattgg ctcagcactg catccacatt agccatgacc
    11401 acgttaaacc aaccgaagtt ggcaccactc atggcgtatg cagcttccac taaaccagca
    11461 gggtaattaa aacccagctt gccgtctttt aagaggcttg ccacatagtc tgatacatcg
    11521 gcaaaggcat tatgctcaag ttccactaac tcaaagcggc gtgcgaccga ttgaatttcg
    11581 cgcaattgct ggccaagcag tggtgaacac agcgctacat agcgcaacca tggaagttta
    11641 cggcgagggt cttcctcttt aattgcttta ccaatcaggc gaatggcctg gccatcgagg
    11701 cgtttatttt gttcatcttc caggccaaag gtggcggctt ctgcaacggt ttccagctca
    11761 tccagcacca ccaacatata ctgcacgcca aagctcttta atactttata ggcggcttgc
    11821 accaaactga ctaccagcgt ttggtcagta tagaggtctt cttcgctgtg gttggcgttc
    11881 agctgtaagg catctgctaa cagttcagat ttaaagccaa atggcattaa gcgttcatag
    11941 gcttgtttgg caatttcact ttgaatcgat ccatcaaagg tttgggtggc cagtggtaat
    12001 agcgcctgat ataaaccaaa agcaaaccag ttatccgagt tttgataatc tgatgccact
    12061 tgcgagtagc gaatgtagag cgccaggtat ttgtcttgcg tgtcgcgatt aaacagctgc
    12121 ttatcgtgca gttggccttg ttcgtcgcgg acaaaccagc cccttgaaca gtcgttaatt
    12181 tgtgcgacca attcatggcc taaacgtgat ttaccccgac cccattcggc ttcgacggca
    12241 aacacatggg caaagcgatc atcgtcgtga tccaccgtat gcaaaaaggt ttgaaagcgt
    12301 ttaaaaaagc ggctctggcc cactaacggg tcgcgaggtg gataatctga tacgcccgtc
    12361 gccgcccagc gatgttgttt tgccaggcta tcaaccatct gttcgtttaa ttcaaaactc
    12421 ataacgacga tccttgaagg tttattctat tggcggcgcg ttttttattg gcatagcagt
    12481 tttgcgccag ttgcttaatg gcccgttgta acgtttggct tttgtagcca aatagtggtg
    12541 ctaacggcag gctataacag gcatcgctga agcttgtatg aattcgatat tggccggact
    12601 caccacgacc cgcctcggta aattgccaga ttttttttgc caacaattcg gcaatgaatg
    12661 agctcaggac gcggtttagg cggttgatgc tttccgtgtt cgcatcgagt gccggataga
    12721 gggggatatt gagctcaccg agcagaagca caagaaactc ggccaaacta cccagactca
    12781 cctcatcacc ttcaatgccc tgtaccgcca catttatttg accgggggca aaggcatttt
    12841 gcccggcagg taaggtgagc aacaaaccgc cacgttgctc ggcagcccag gcatcttgca
    12901 caaagataat tagactgagt aaaaataacc agggctgttg ggcgaatagc tcggtaagcg
    12961 gcacgttgtc atgacccgag cgggacacca ataaccaatc ggcattgggt ttaaggttat
    13021 gttcatcaag caaggtatta gggagtgcta acaaccgccc actacaccag ttatttgtca
    13081 gttgttttgc ggtgacatcc accgcttgta tagatgccgg tggttcattt tgttcgctca
    13141 cggtaaacag ggtatggctg gtgcgcaaaa tacggcatag agcgggaatg gcagcattgt
    13201 cgtttaggct atgaccgagt aaatcccgtt cggtttgcgc caatgggctt gcaatgattt
    13261 gtggtgagaa gtctgcgttt tccaactggg ctaagaccca ttcactggca ttgcccagtt
    13321 gctgaatgag tttaaccaat accattgggt cactcatcgc gccagcttcc tggcagcgag
    13381 cggcggctaa atttatccaa tgttggcgaa catctgggtt taagcccaac aacgctaatt
    13441 gccattcacc cgactggctt aaatcactaa aggcatcaag taatggtgca gtgaggctaa
    13501 aggcgctaat gatatccgtt tcagcgtctt tctttttcgg ctgtattatt ggttgtaaca
    13561 gtgacctgag ggcaggcaca gccgccagta acaacgctgc tttactggcg gttaaaccgc
    13621 cttgatgcca aagctgcagg cagaggttaa gtgccgcttc gctatttacg gggtcgttat
    13681 tttgcctggt tgtaatgctg tccatgcccc ttgtctccat ctttactctt cagcctccgc
    13741 ttgcgccgcc tcttccaaca aactgtataa ctcatccaca tcgctaaata gcggtgtggc
    13801 gccttctatg gcataaccct cagcgctcag tttacttaaa atcgcttgat caaagctgtt
    13861 gtcgccatct tccagggctt gttgagcctg gattaattgt tgctcggtca gttgaccttc
    13921 gatgagggct tcgacgaggg ttttggctac ttgtgttaaa aatttggcat aacgataagc
    13981 cagcctcgca ttgagcaaat aatcagcaag cttgttttct aagcttttat ccagtcgagg
    14041 aattagtacg cgaccaatat cagataggga agtatgttct acagttgttt taactgccca
    14101 ttgatttagc tgcatttgtc cttcggggcg atttagccaa tgtgttaata gttcgccacg
    14161 aatattttga ggtgagagca ttgcaacccg ttgattaacc agcataggta gctcgtcaga
    14221 tatgaaaaac tgcgagcgga aaataccgtc cataccgaca acgatcattt tgtctttagc
    14281 ttgatactta gtcgcatcct gatatacatc ttggctaacg ccagtatcta aaccaataaa
    14341 atcatcaaaa ccgatattgc ggatgcgaac taaaggtatg cctgagtttt ccaaaaagtc
    14401 gttactggat actgggcaac cagatgccaa ttcaaccaaa gtgctaatgc tgtcatgagg
    14461 tagtttttca atttcacgca ctaaacatag gtagtgagtc cgataaggac gtggatcgag
    14521 tcgatcctcc attgtttgcg cgctgactcg gtttaatagt gcggggggct cgttgatcgg
    14581 cagatttaga ctattaagat gagcgtcaac tgatgtgcga agcagcttcg cccaagcgcg
    14641 cagctgctcg gcttggcgaa ctttatcccc gatgtatttt tgaacaaact cagacacttt
    14701 cggaataggt aaacgtggaa catctaacaa gctcactcca ggaataatag tcccccggcc
    14761 tagtcgctta aaagcctttt gtccttggtc acagttcagg tagctaacga gatagtaagg
    14821 atcgctagag gccttcattt tcaggtatat gacttctgaa gaacagtttg cttcgttaac
    14881 ttcagatgga actacgcacg catttcccat atctcctttt ctaataacta aaacatctgt
    14941 tggtttgagc gctgattttg taagctcatt tttatgtgaa tcgagagata ttcgttggca
    15001 ttcatctaaa tcaatattga agcttttaat tacctttccc gaaatatatg gaatactatc
    15061 agcagcttca gagtaatacc gttcgactgc tgacgtgttt ccaataccac tcgcttcata
    15121 aagtgactgg agttccaccc agcctttgga ttttatcagt ttgtcatttt ctactaggct
    15181 cggttgataa aagttaggat caagtctttc cacaagaatt gagctgtttg ttcgaaaaaa
    15241 tttgtccatc actcaccccg cttataagcc gatacaatct tatccagatc gtttgcgaca
    15301 ccagcgttat agtcttcaat attatttttc accacgtagc ccaaggtttc agccactgcc
    15361 ataaacacgt cagtttgtgg ctcgggcaaa aattgctctg gctggttggg gttagcatgg
    15421 cgtttttgca aatacaaaat cgaggttttg gcgcccgtgc cagagagctt aaagcagtcg
    15481 ctcggcaatg aaattactgc ttttacaatg gctttaccgc caacaaactc gccggttttt
    15541 tcgtcttttt tccccatgat gtactcgcgt acataacggt cgccggagtt acataaaata
    15601 ccatcgggca gcactattaa cagacggcct ccgggtttaa gcagttgcag gcagcggtcg
    15661 ataaaaagta ctgctgggtc aatagtggca cctaccggtt gccagttgcc cttgctatcg
    15721 ggcttgctgc ccagcgcgag ccctgcggtt gtaggctgca ggctgtaaaa cggcttttta
    15781 tcagtcccgc ccgttctgcg caaatcggta cgatagcccg cccatttagg ctcgccgttt
    15841 tcatccagct ctaaatcacc gatatcctcc cacttcattt ttacgtggtc ataccaggcg
    15901 ttgtagctat caacgacttt tttggtcgga cgaaaaccac cgagcacttg ctccatattg
    15961 gcttcgtaat tggcttttga gttttgacca tttttgcctt tatcaaattt aggcgtaccg
    16021 aacggcgggt tggtgcaaat aaggtcaaag ctattcggct tcagggcgtt agtggtgagt
    16081 gagttgtcgg tatagaaaat ctgtgctttg ggggcacctt gcaatgccat attcacgcgg
    16141 gccagcatta ccatgcgtgg tgcagcatcg gcaccggtaa atgcacagtc gcgtaatttt
    16201 tgtttcaatg attctttttt atcgtcggcc agcaccagct taaaatcgat ccatttatca
    16261 atctggctta acgccaccga gccaaaacca aaggagccac aggtggggtc gcagaaacga
    16321 aaatcgccat tggctaagcg cgatcgcatt tcatcgtcat cgtcgatgtc gtgcatagca
    16381 atttctagca tggcctgttt tactgggtta ggtgttaaat aaacgccaag gccacctttg
    16441 gattcaaagt tagcgcgtag gaatacatca aacactctgc cgagtaaatc tcctgacaca
    16501 tcagccaacg tgccgtgttc tttagcgatt ttaccgtcgt tggtttttac cggtcccagg
    16561 ttttgtattg cttccagcaa atcctgatag ttttttgcaa cagacagccg caggtgtgtt
    16621 tctttactaa agatggggtt acgtgagcca tcatctgaga tcacaacgta gttttcgtgg
    16681 cttttaaatt cctcaaaggc ggctttgatt tgggcaacgg ctttatcgcc gtggctctcc
    16741 acatattgcc aattaaagac atcacgaaaa cgcagggtat ttccctcatc atctttaaag
    16801 gtaaggtctt catcgtgatg caggcggaaa gtttctaaaa acagaagctt agctacttcc
    16861 tcaataatgt cgttcttgtc gtttaccttg tctttggtaa cgcgataaac attttcatgg
    16921 aaggcatcaa atcgctgcat taagcgtgta taaacacgca tcgaccagcg aaaggttgga
    16981 tcacgctcca tgcgatcaaa gctacgtaat tcttccagac caggcagggt atctgcaccg
    17041 tgggtaccgt cagatccatc ggcaccgaac aaggccaaaa tacggtggtt caagccatcg
    17101 tccacaacgg catatactgg ccagtcacct tcgatatcac ttaacagctg ataggctaaa
    17161 tcatcaaact ttgcagtgtc agcggcgtat tcccaatggc cttctgtcag cgcaatcaac
    17221 atgactggtg gcgttaagtt gccttcaacc aggccttgaa tgagaccagc cttggcgaat
    17281 tcatgctcgg gtaattcgac caatggctga gtaaactcgg agtgctgttt gaccagttta
    17341 ttgaacagtg cttctagctg ctgttgcggg tcttgttttt ttgcttcttt tgccatgatg
    17401 ttttctaaaa tcctttaata cagccaatgg ttactacaaa tgccggctaa tttatcagta
    17461 ccagcaaggg gcctttaatc tctatctgcg cgtgcttctg tgccagtttg cgtatctgct
    17521 gttcactggt cgtaatacca gcagggagct ttttcaatac ttgtgcaagg ggcatgcgcc
    17581 caccagcttg atgcaacaat gataccaaat gtgccgcgat atcaggtata acctggtacg
    17641 ttgccgttgt gggccctttt agatgattac tttgaggcaa tgaggcaagc catagcgaaa
    17701 cttgagactg gctaaagcca agtgtttgcc agagggtttg gttctcatta aatacaacgg
    17761 cggcgcttct gggagttgct gtttgctgag ttattagttg ttccaggctt tgtcgctggt
    17821 attcatgggt catctcatgc ctccactata ggatactgaa gcagccatcc gatccagttg
    17881 ggggctcatt gtacaggaca cgtcattcgg ccacatcatc ctctcaccgc gttttgccat
    17941 tatccggaat ggcacaaccc ataattttaa agtctccgag aaagcaaaat gctgcggtat
    18001 cggttggcta aagtcatcat gcgcagggta aatcagatac atttcgccat tcccaccaag
    18061 atatttttgt ccgtaggcaa acatttgata gaagtcagct tgtgccagtc catagagtga
    18121 ttttgtctgc tggctgctat tcaccagctt ccattttgta tccatcacca tttcggtttg
    18181 aactggttta tttgattgaa tcagcaagtc ggggcgaagc ttaaagcaat ctttaactcc
    18241 atgctttacc aaagaataaa ttgctgcttg aggtaaaact ttgaggtgag gtggtaactc
    18301 gtcgggtagg gtctgtgcca caaaggattc aaataccgct tccataggga acaaaagtga
    18361 tatcgctttg gtgtttccct gcaaagcact cgggctcatt cccctcagga tcaattgcgc
    18421 ccatgccatt ggctcgttgt aatgagccat accacgctcg aggcgtaagt tgtttatatc
    18481 acgttcaata tcccggctaa gtggaatacc gtcaaacgca aaacgcagtt cgtaaagcca
    18541 gcgttgatta tctgatgaca gctgcagact aagaagctta tctagtgcgg agtgtaagag
    18601 ccgattggct gcacaatcag gcatgtagtc atcataatcg acacaaaact tatggcgatt
    18661 caccgcgtta tgtcgcagct gtgcagaaag catcagcttg cccttcataa aagccagatt
    18721 accttgcttg ctcacatagt cggagcgtaa accctgttta agcaattggc tgacgctatg
    18781 caaaaactgg ttaataaaaa tctcaagcaa aggcatacgc tgagcctgaa gagtggcttg
    18841 ctgggtttga atatgtcgga acccaggcag atggcttagc atcgtcagca atgtttgacg
    18901 tgcattagcc gctgtgaggt ttttaccaac cttgggcagt atctcaagct ggaaaccgtg
    18961 aggtgttgaa agcatccctg cgtagttttg aacctgaagc aatttgaagc ctgagcgaga
    19021 ggtgagtttg aggaactggc tcccttgatt acttgccagg ctaagcgctt ctaaataatt
    19081 aaagacctgt ggaggcaccg gttttgcgcc aactgaagcc gcttttccac tccccaagag
    19141 gtcatattca aaaacggaga taacttcacc catgatttac tgcctgctca tcaaggggag
    19201 tctgttgggg ctgataaatt gaacgataag cctgtggaat attccagatc tcttgatcga
    19261 aatctttgag ctcataagct gttgattgct gatcatggcg tcgtaaacca tggttgttac
    19321 caaaaagcgt atcgagatcg tcggttttct caatcacgaa ttgcaggttg tcgtcttgct
    19381 tttgattgtc tgccagcacc aaccggatct tgttccagtc atcgaaaaag tattcctgta
    19441 aaagcggaat gatctttttc tggaatgcga tcttcaattg tttaaacgca gcttcttcat
    19501 caccggcatc gagtgcattt tttaccggca taaagaacgc atgccccagc gtatgttcac
    19561 gatcgtaaag agcctcgatg cggctattga gtttctctaa caacgactcg agctctatgc
    19621 ctttcacctt agcttcactc agtaaagaga gatcaggcat catttcgaca aagtcaaagc
    19681 gacggcgcaa agccgtgtcc atcagagcta aagaacggtc cgctgtattc atggctccga
    19741 tgatatcgac attgccgggt acgctgaagt gatcaccgct ataagccagt tgcaggctca
    19801 tcgcattggg catgcctgca cgcttgtcta cttcaatgag tgagattagt tcaccaaaga
    19861 tcttggatat gttaccgcga ttgatctcat caatgaaaat ggcgtagcga tgtcctggat
    19921 cggcattcgc acgttggcaa aggcgcataa agatacccgg ctcaatggga taagagatat
    19981 tgccactctc gtcagagcga gcgcgtatac cttcaataaa ttcttcatag ccgtaagatt
    20041 ggtggaacgt aaccaccgca aaacgctgga tggcttcggc agatttaggg ccacgtttaa
    20101 gttcggcgta gagctctacc aaatcctcca cttgctcgag ctgtgaatca actagaaacc
    20161 attcgctgtt atctgttttg ttaaatacgg caggctcacg ccgattctta taatcaacgg
    20221 ttaacgactc gggaactgta taggattgca aagctgccca ggcagtattc gatagattgc
    20281 cattacgacc gtttaataat gccttacgtt gaaaccacat atgttcaata atttggcgaa
    20341 ctttcgctcg tttcccaaga tcgagcagca ccagcgttat aacctgcatc cagttcaacg
    20401 attcaaggcg agaatccagc taggcatcac gatcagcggg aacagcatga gaagtgtact
    20461 ctttcatttt ttgctgcaac ctgaaggtct tgccagtgcc aggggggcca aagaagatca
    20521 cgttagttgg tgaatgtagg atgatcttgt tctcgatggg ctcactgcgg gttatggatg
    20581 tagtgctagc tactttctcc tgcttttgtt cattatcgcg ttcacggagc aattcataga
    20641 gatgccagat ggccatattc acataataag gatctgtatc aggagattgg gcgtgtatgg
    20701 cttgtttcaa ttcatagttt ttctgtaacc aatttccggt cagtaccaaa ccgagatgga
    20761 agtgctgatt taagaattct gctgctttat taaaagcatt ctcatccaca gtactggtgt
    20821 agttttctgg cgagaacgcg gcaaagacgc gatttctcaa actccaatac atacgcttga
    20881 gaagtccgtt ttctttagct tgttgaagtg cattgccaac atattggtaa gtttcttcat
    20941 ccggctgttg ccgaattcgt tcagttagtt ctctaagcag tggcaggctt tgctgatatt
    21001 ctgctaagga tggaacgccc tggcgaatac ttgcaatgcc gttgtcacgc tcataccaaa
    21061 gctgtttgac cagaccatct gttgcaggct cagcaaaagt tgaggtttgc tgtagcaaag
    21121 tcgtaaatgc acggtatcgc tgataccatc tggaagctgt gtaattttta tctttcgcca
    21181 gggcatcaat caattcacta atcgttagcc tacctgcttc gacactatct acaagagtca
    21241 tcgtgacttt tcctctgttg tgacattact tattttttaa ttaatatgag gtattctaac
    21301 tatattgagt taatcgttgt gacaagtgcc tgttatatat acaactatct gttaactcag
    21361 ctctaattta aatacaatgt aaggttgtag ggtgaatgct gttatcaacc tcacccccta
    21421 aaaacctctt tccgatgcca ctctacaaac acctcataag ggtaattttt acgcacctgc
    21481 ggcaacgcaa tagttttccc gtcaaaatcc cagaataatc tctcaacgat ccctccgccg
    21541 tttaccgcat ctgataccag tacccgcata ttttcatcca ggccaatcga tcctttatca
    21601 aaggctttat ggtgaattgc gcaaagcgcc aggccattag gaatttcaca aggacctcct
    21661 tgctgtttcc attttatatg cgccgcttcc aacgctacgg tggtgtcgtc gtggcgcata
    21721 ttaaagccac atatcgcgca ctggtagtta taagcgcgta atacattttt gcgaaataaa
    21781 gggtcgcgct gtttacggat ttgctggaga tcaaaaccca gctcgtcagc aatttcttcc
    21841 tgaatactct cggtgaagtg cgcttcgagt atctgttgcg ccagagtatt gatgagtttt
    21901 ttattgccgg ttaccagggc gtagtgctgt tcatcgaaac cgcctgcaac gtggtattcg
    21961 ttcagttcct tcactggcgg ttgtcggctg ctccctgctg ttgagcaaag ctcggcattg
    22021 tgtaattgcc agaatccgtc gccttgtaat cgccagaacg gcatatcagg tcggtaatgt
    22081 gaacgctgtg gcccaaaacg ttccagtaaa ctgtgcagag gttcgtagat ttccgatcca
    22141 taatcgaaaa ggcgtggatg tccattcagg tatcccgcta atacgtatag caataacaat
    22201 ggcttatgcg gcgcgcgctg ttcacctttg tgccagattt ttatgttggc aattgcctgc
    22261 tgtagcgatt tactggaagc cattgatatc cctggcagtg aaataattgc gatcatgctc
    22321 gcaaattttc ggctgttcaa gcctgcaagt gaagatatat tactttgatc tcatttgttt
    22381 ttttgctgcc tgtgtaccct aattgccatg cgatgttgca ccgacgcaaa ccacctttta
    22441 cgccggagga gttaaaagcg ttgatggatg cgaataaatc aaattaatga acgctcactt
    22501 ttcgcgccaa aactgcaaaa ttgcttctac aaacctgtca gaaataaccc cccgacgggc
    22561 tgccgtgctg aaatgactgc ttcgcagtgc cgtcgtccag ttctccggtg accggtacgc
    22621 tgaaccgtaa tatcccgtgc ttaaatcgtc cgggtcttca tcaggcgcgt tcgtcagtcc
    22681 tctttcacct tgttgctgga agtcgacgaa aataatattg gctaaaacat tattttgata
    22741 attgccataa atagcttcat acgaatgcgg gaaattttct ttccagtacc aggtggtatc
    22801 gccgcaaaac cacggcgcgt cagtaatatt attaagctga gaatggtatt gttttagatc
    22861 cctacgaaag gcttcaacca tatgattaaa gtgttgaggg tgtgacgcgt agtcactggt
    22921 cattaagtca aattcgcctt gcatccagca tacgccgagg aatttgttct gcggattttt
    22981 tgccagtgcg gctcgcgttc tgctgactaa atcctggtat agcggagtat ccgatcccca
    23041 acgacaagca tcatggctgg ctccgtgccg ttctgaatat gtcccttcgc tgcccgcgat
    23101 aaaagccgag ccgccacgac aacatggaac gatgagaacc cctgcattat caggaataga
    23161 gggcagtaat ttccgtgcaa tatgcagtgc ctggccaacg gtgccgtact gtgtttgatg
    23221 attcgttgcc agaggatggt gataaccctg catatcctga acatcgtgtg ggcagtgagt
    23281 cagtggaata atgtcgttaa agtgacatga cgggcctccg ggatgcgtat gcgcaaatct
    23341 cgctaattgt ttaattctgg gatgaggcgc atcttccctg tccggtaatg gcagtccttc
    23401 gccatacgcc atggcattag actgaccagc aacggtaaga acatagtaat aatcgggcga
    23461 tattattgcg ttcatataaa tttcgcgtaa ttgaaatgac aaattgatag cagggcccct
    23521 ttaccggggc cctgaagcgt taattttgta ctgtgacttt attatccttc acagagatca
    23581 aaactgaatc cgtcaccgct ttgccgcctg cagtttcacc gccaataatc aatagactat
    23641 tattccaggg caatgatact ccgtaggccc gaccttgcga taattcaccc gatttatccc
    23701 atttcccgtt atgccaaaga tgaatatcag tgctatatga tttttttagg ccttcatgcg
    23761 catagttctt accgttctgg taattttctc gtgaaccttt gaatccggcc cctccggcaa
    23821 atataagaga atcattgctt atccccgcaa aaccgccagc gacgccatct ggtgatgaga
    23881 cgggagcaag cttattccat tttaaattat taccggtgaa atcaagttca aatacggcat
    23941 ccgttcgcaa tcctggtttg gcttcgccat taataagcca ggttttatca cctttattca
    24001 caagcgccgc accagccgtt ccgtaccagg gagattcgcc agcgtaactc cattgctgtg
    24061 ttgagggatc aaaagacaac agaaacttat tgaagaaata atcttctgct tttttgtcaa
    24121 aataatgggc attgatcttg tctatagttg ctgaatcttt tccagcctcg ttgagatctt
    24181 caaaatagcc attgaagata ttctggttaa caccgccagt aacataagcc ttgccattgt
    24241 gtacaaaagt cacatgaccc gccatgccca tcggtgcatg cgacatcaat ttaacccaac
    24301 tattggtttt ggggttgtat ttgtgtacgt cattaaatac ctgagtcaag cccttgctat
    24361 ttttgccaat gccgccaaac acatacagat tgccatcaat aaatgccgag gttgcttgat
    24421 ctcttggtcc accagggaat gcagctaacg ctgtccattt tttatctttg gcctgtgtat
    24481 ccagcttgta ccatgccgta cctgcgctac ctaaaccaat gtagacagtg tcgttatcaa
    24541 ttgctccggt accactttta aatggcacag gagtttcagg taatacagac gcgtttgcgg
    24601 caaatgaagc catcatgata gcaagcgccg ttattgtttt attcattgtg actgtctcct
    24661 gtctactaca gtttaaatga cacaccaatg cgataactgt tttccgataa attatctctg
    24721 ccgttgtaaa caccctgacg gtccaggtag tcatattcta tgtatggcgt aatatcgggc
    24781 gtcatatggt attgtagaac aaatgcattt tccgtcgccc atttcttatg gtttgcatag
    24841 cgataatcgt tctgtttgct gtatagcgtc gtttgccatg cgaaggtgaa atcactatta
    24901 atatggtaag tgacatatcc atcccaacga tgaacgttat cacgagacat atcaccggat
    24961 aagtcttgtt gtcggtaagc tttccagtcg taacgatagc gaatgccaaa attaagatct
    25021 tttgtcgcgt cccaggacag ttttacgtag ggtccgtagc gtgtgccgtt gctgctaaaa
    25081 tgcgttaaca ttcccgggcg caccgtccat tgatcatcaa gtttaatcgc gtaattaact
    25141 tcaacctgaa catcattgag tgcggcattt tcctttttat tatcatgaat ggtattccag
    25201 gtattacttt ccatgcttgc ccaccatcca ttttgccatc cctcactgac tttgagtcga
    25261 gtctcatagg cgtggcttcc actacgatat ccaccacgta cgtccagtgt tgcagcctga
    25321 gaaattaatg gtgacgaaaa gcacagtaat aatacgccag aaagtatttt agcctttttc
    25381 ataaatttca ctcatttgta ggatacagaa agcaatacaa agcccgcata aacaattagc
    25441 atttatgttg tgtagtattt tttgccaggc ttatagtgtc tttggcaacc gatagctgta
    25501 ttttatattt tttgtataat gtctcctgtg aaaaatctct ttccacatta tttaaataaa
    25561 cagagagcca gattaaatac ttgagtataa aatctctgct gatgtttaat tgatttgaat
    25621 gttcgtaagc tatatcactt actcaattca ttttacccag agtcataaaa tagagtgtct
    25681 gttgatctca ttttcatatt cctttcagac tgaagtgcat tttcatcacc cgatgagaaa
    25741 gaactgaagt gatgtttgcc atagattacg agatgcccag tctcattgcc tatcgtggtg
    25801 cacctttata cctgttatac cagatcaaaa atcacgcaat ccatacaacg aaaccagatt
    25861 tgcaattcgt gtcacaaaat atgtcgatct ttttctaaga agaagatgcc atgtgaagcc
    25921 agacgaacac ttgcggaggc cttcacaaac caaagattgt tgtttaatta tctgttttat
    25981 aagtaattta tttaaagtat aaacaaggaa agatttgcaa gctaactcag tgagcgtggt
    26041 gaaaatcagt gtttacccgc catcaggctg agcataattc tcatcatgaa atatgtttcc
    26101 tggtttgtgg cttgtaactg gtcacttctg aagtcgatct ggagaggctt gttgatgttg
    26161 gtgttttcag gatgatgttt cacttagttt gtttgccgta tcgcccggcg aatggctgtg
    26221 attgaggaag gtttaagtcg tagtgaccaa agctatattt accaacgaat gtagatgaaa
    26281 aaatcatctc ctgcgttccc catatctcca ggataaaaag gaatgtaaca atctcatggc
    26341 gtaagctgac gaatcagcca ggaataatcg ctagggacct aagaattagc atgataatag
    26401 ccactaagaa attactgcgc tccatgaaat agccattttg tggcaaatgg agttgactaa
    26461 taatgtcata tgtgagacgg ctagttgaac gaatattaaa ttttgctgaa ttttttatgt
    26521 tgattttact tgttacagaa catatcacat gatatataga taagattagt tgcattaatg
    26581 atgagggtta ttattagatt cgtatccgat tgataaatat ataaaggtac atagcatgca
    26641 agagcatggc gtttgtatgg caacgttatt ataattaact gttgctactc catttaagtt
    26701 cactcagaag aactggtcca cttacgttag ttattaagca aacgttcgct tttataaaca
    26761 taatcaggat aaaaatgttg gattattgct aacccagcac agctagtgcg cgtctgtaat
    26821 tataagggaa aaacgatgaa gaataaggct gataacaaaa aaaggaactt cctgacccat
    26881 agtgaaatcg aatcactcct taaagcagca aataccgggc ctcatgcagc acgtaattat
    26941 tgtctgactt tgctttgttt tattcatggt ttccgggcga gtgaaatttg tcgattgagg
    27001 atttcggata ttgatcttaa ggcaaagtgt atatatatcc atcgattaaa aaaaggcttt
    27061 tcaacaacgc atccgctatt gaacaaagaa gttcaggctt taaaaaactg gttgagtatc
    27121 cgtacttcgt acccgcatgc tgagagcgag tgggtatttt tatctcgtaa gggaaatccg
    27181 ctttctcggc aacagtttta ccatattatc tcgacttccg gtggtaatgc cgggttgtca
    27241 ctggagattc atccgcacat gttacgccat tcgtgtggtt ttgctttggc gaatatggga
    27301 atagatacgc gacttatcca ggattatctt gggcatcgca atattcgtca tactgtctgg
    27361 tataccgcca gcaatgcagg gcgtttttac ggcatctggg atagagccag aggacgacag
    27421 cgtcacgctg ttttatagag atagttgaga taccagggat ggtgttaaaa actacatt
//
LOCUS       DAZGAN020000056        27446 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGAN020000056 DAZGAN020000000
VERSION     DAZGAN020000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.056"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(163..909)
                     /gene="cutC"
                     /locus_tag="ACHKYO_RS13370"
                     /old_locus_tag="pgaptmp_002674"
     CDS             complement(163..909)
                     /gene="cutC"
                     /locus_tag="ACHKYO_RS13370"
                     /old_locus_tag="pgaptmp_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGU4429498.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(923..1489)
                     /gene="yecM"
                     /locus_tag="ACHKYO_RS13375"
                     /old_locus_tag="pgaptmp_002675"
     CDS             complement(923..1489)
                     /gene="yecM"
                     /locus_tag="ACHKYO_RS13375"
                     /old_locus_tag="pgaptmp_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4429499.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1705..3438
                     /gene="argS"
                     /locus_tag="ACHKYO_RS13380"
                     /old_locus_tag="pgaptmp_002676"
     CDS             1705..3438
                     /gene="argS"
                     /locus_tag="ACHKYO_RS13380"
                     /old_locus_tag="pgaptmp_002676"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGU4429500.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPANELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3480..3524)
                     /gene="yecV"
                     /locus_tag="ACHKYO_RS13385"
                     /old_locus_tag="pgaptmp_002677"
     CDS             complement(3480..3524)
                     /gene="yecV"
                     /locus_tag="ACHKYO_RS13385"
                     /old_locus_tag="pgaptmp_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HGU4429501.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3615..4103
                     /gene="yecT"
                     /locus_tag="ACHKYO_RS13390"
                     /old_locus_tag="pgaptmp_002678"
     CDS             3615..4103
                     /gene="yecT"
                     /locus_tag="ACHKYO_RS13390"
                     /old_locus_tag="pgaptmp_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HGU4429502.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4223..4615)
                     /gene="flhe"
                     /locus_tag="ACHKYO_RS13395"
                     /old_locus_tag="pgaptmp_002679"
     CDS             complement(4223..4615)
                     /gene="flhe"
                     /locus_tag="ACHKYO_RS13395"
                     /old_locus_tag="pgaptmp_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HGU4429503.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4615..6693)
                     /gene="flhA"
                     /locus_tag="ACHKYO_RS13400"
                     /old_locus_tag="pgaptmp_002680"
     CDS             complement(4615..6693)
                     /gene="flhA"
                     /locus_tag="ACHKYO_RS13400"
                     /old_locus_tag="pgaptmp_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HGU4429504.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6686..7834)
                     /gene="flhB"
                     /locus_tag="ACHKYO_RS13405"
                     /old_locus_tag="pgaptmp_002681"
     CDS             complement(6686..7834)
                     /gene="flhB"
                     /locus_tag="ACHKYO_RS13405"
                     /old_locus_tag="pgaptmp_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HGU4429505.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8036..8680)
                     /gene="cheZ"
                     /locus_tag="ACHKYO_RS13410"
                     /old_locus_tag="pgaptmp_002682"
     CDS             complement(8036..8680)
                     /gene="cheZ"
                     /locus_tag="ACHKYO_RS13410"
                     /old_locus_tag="pgaptmp_002682"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HGU4429506.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8691..9080)
                     /gene="cheY"
                     /locus_tag="ACHKYO_RS13415"
                     /old_locus_tag="pgaptmp_002683"
     CDS             complement(8691..9080)
                     /gene="cheY"
                     /locus_tag="ACHKYO_RS13415"
                     /old_locus_tag="pgaptmp_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HGU4429507.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9095..10144)
                     /gene="cheB"
                     /locus_tag="ACHKYO_RS13420"
                     /old_locus_tag="pgaptmp_002684"
     CDS             complement(9095..10144)
                     /gene="cheB"
                     /locus_tag="ACHKYO_RS13420"
                     /old_locus_tag="pgaptmp_002684"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HGU4429508.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10147..11007)
                     /gene="cheR"
                     /locus_tag="ACHKYO_RS13425"
                     /old_locus_tag="pgaptmp_002685"
     CDS             complement(10147..11007)
                     /gene="cheR"
                     /locus_tag="ACHKYO_RS13425"
                     /old_locus_tag="pgaptmp_002685"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HGU4429509.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11026..12627)
                     /gene="tap"
                     /locus_tag="ACHKYO_RS13430"
                     /old_locus_tag="pgaptmp_002686"
     CDS             complement(11026..12627)
                     /gene="tap"
                     /locus_tag="ACHKYO_RS13430"
                     /old_locus_tag="pgaptmp_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HGU4429510.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12673..14334)
                     /gene="tar"
                     /locus_tag="ACHKYO_RS13435"
                     /old_locus_tag="pgaptmp_002687"
     CDS             complement(12673..14334)
                     /gene="tar"
                     /locus_tag="ACHKYO_RS13435"
                     /old_locus_tag="pgaptmp_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HGU4429511.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14479..14982)
                     /gene="cheW"
                     /locus_tag="ACHKYO_RS13440"
                     /old_locus_tag="pgaptmp_002688"
     CDS             complement(14479..14982)
                     /gene="cheW"
                     /locus_tag="ACHKYO_RS13440"
                     /old_locus_tag="pgaptmp_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGU4429512.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRVYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15003..16967)
                     /gene="cheA"
                     /locus_tag="ACHKYO_RS13445"
                     /old_locus_tag="pgaptmp_002689"
     CDS             complement(15003..16967)
                     /gene="cheA"
                     /locus_tag="ACHKYO_RS13445"
                     /old_locus_tag="pgaptmp_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HGU4429513.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELIITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16972..17884)
                     /gene="motB"
                     /locus_tag="ACHKYO_RS13450"
                     /old_locus_tag="pgaptmp_002690"
                     /pseudo
     CDS             complement(16972..17884)
                     /gene="motB"
                     /locus_tag="ACHKYO_RS13450"
                     /old_locus_tag="pgaptmp_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
     gene            complement(17881..18768)
                     /gene="motA"
                     /locus_tag="ACHKYO_RS13455"
                     /old_locus_tag="pgaptmp_002691"
     CDS             complement(17881..18768)
                     /gene="motA"
                     /locus_tag="ACHKYO_RS13455"
                     /old_locus_tag="pgaptmp_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HGU4429514.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18895..19473)
                     /gene="flhC"
                     /locus_tag="ACHKYO_RS13460"
                     /old_locus_tag="pgaptmp_002692"
                     /pseudo
     CDS             complement(18895..19473)
                     /gene="flhC"
                     /locus_tag="ACHKYO_RS13460"
                     /old_locus_tag="pgaptmp_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
     gene            complement(19476..19826)
                     /gene="flhD"
                     /locus_tag="ACHKYO_RS13465"
                     /old_locus_tag="pgaptmp_002693"
     CDS             complement(19476..19826)
                     /gene="flhD"
                     /locus_tag="ACHKYO_RS13465"
                     /old_locus_tag="pgaptmp_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HGU4429515.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            20606..21034
                     /gene="uspC"
                     /locus_tag="ACHKYO_RS13470"
                     /old_locus_tag="pgaptmp_002694"
     CDS             20606..21034
                     /gene="uspC"
                     /locus_tag="ACHKYO_RS13470"
                     /old_locus_tag="pgaptmp_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HGU4429516.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(21041..22465)
                     /gene="otsA"
                     /locus_tag="ACHKYO_RS13475"
                     /old_locus_tag="pgaptmp_002695"
     CDS             complement(21041..22465)
                     /gene="otsA"
                     /locus_tag="ACHKYO_RS13475"
                     /old_locus_tag="pgaptmp_002695"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HGU4429517.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(22440..23240)
                     /gene="otsB"
                     /locus_tag="ACHKYO_RS13480"
                     /old_locus_tag="pgaptmp_002696"
     CDS             complement(22440..23240)
                     /gene="otsB"
                     /locus_tag="ACHKYO_RS13480"
                     /old_locus_tag="pgaptmp_002696"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HGU4429518.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(23407..24393)
                     /gene="araH"
                     /locus_tag="ACHKYO_RS13485"
                     /old_locus_tag="pgaptmp_002697"
     CDS             complement(23407..24393)
                     /gene="araH"
                     /locus_tag="ACHKYO_RS13485"
                     /old_locus_tag="pgaptmp_002697"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HGU4429519.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(24408..25922)
                     /gene="araG"
                     /locus_tag="ACHKYO_RS13490"
                     /old_locus_tag="pgaptmp_002698"
     CDS             complement(24408..25922)
                     /gene="araG"
                     /locus_tag="ACHKYO_RS13490"
                     /old_locus_tag="pgaptmp_002698"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HGU4429520.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(25992..26981)
                     /gene="araF"
                     /locus_tag="ACHKYO_RS13495"
                     /old_locus_tag="pgaptmp_002699"
     CDS             complement(25992..26981)
                     /gene="araF"
                     /locus_tag="ACHKYO_RS13495"
                     /old_locus_tag="pgaptmp_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HGU4429521.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
BASE COUNT         6708 a         7419 c         6592 g         6727 t
ORIGIN      
        1 aatattatta gcacaacgct atgttcactg tcacccgctc aacagatcac ggttatcgtt
       61 cgttttttat actgctcagg gataaaaaaa ggcccctgtt gaaattgcgg gggcctggta
      121 cgagcaagca tcatattggg cgacatgatg caacggtaaa aatcatttgg cctgatggcg
      181 ttcaatgatt cctttcattt cagcaaccgc cgccccgtct acgatataac gcgaatactc
      241 gtccgcgtgt tcatctgatg acatggacaa tccttgatta cgataacgca tcggtgaggc
      301 ttgccacgct cccgcggagc tatggacttc cagcactccg gcatcgagga agtggtgcag
      361 gttttctgca cggactcctg ctccggccat aatgattgga gcatcacgat gggcaataag
      421 ttccataatt tttgataaac cttgcagtgc gtctgatttt tgccctgatg tcagtacccg
      481 ggcaatgccc aattccgcaa gattattgag tgtatataaa gggttagcgc acatatcgaa
      541 ggcgcgatga aaagtcactg ccagcggacc ggcagcagcc attatttttt ccattcgtgg
      601 catatcgaca ttcccgtcaa catcgagaac gcccgtcacc agtccaggaa aacctaattc
      661 gcggaccgtg cgcacatcct caagaatggc ggcaaactca ccgtcgctgt agcaaaaatc
      721 accaccgcgt gggcgaatta tcggatgcac agggatcgtc acccgctggc gcacggattt
      781 cagtaccccc aacgacggcg ttaagccccc ctcttttggg gctgcgcata attcaactct
      841 gtctgcgccg ttttgctgcg ccgttagtgc acattccatg ctgtaacagc aaatttccag
      901 taatgccatt tttgctcctt aattacgctg actgctcgct ggcaacgatc tcttcaatgg
      961 accacggatg aaacttaata gttgttttgc catcggtgac cgccagcgtc ggattcggta
     1021 atcgctcatg ctcacctttt ggtgaactgg ttttaactga gatccccggt aggctgagtc
     1081 cttcatcaga aagcaatgcc agcgcacggg cattcagcgt ttctggatca cccggcagaa
     1141 cgatttcaat atgttcccaa ccttcgtgtg ggtaacgttt ttccccgggc cacggtagct
     1201 ccacaataga aaactgccag tgcgcaacct gtaccggttc atgcaattta aacagacaaa
     1261 tcggtctgcc attgatcata ttttctgaca aaagctcgcc acactgttca aacccgcgac
     1321 gccagcgttc agcagtggcg ttttgatggc aacgcaaaga aatgtgatcg gcagtcagcg
     1381 gagtgatatt caaccccaga cggagggaaa gttcatctaa tgcgtgggta aatcgcggta
     1441 aatccgatgc aatatcctgc agctcgtcga tagattgcca gttcgccata atcactcttc
     1501 gtctttcagt aaaagcgtta atttaccctg ttgccctgcg ccaaccaacc gctgatttca
     1561 cgccgcttct gatgcaatag tgaaaacggc aatacgccac gcgcacgttg ctgacgaaaa
     1621 cagccatttg cagtatactc ccgccctaat ttctttaact ggtgcgggca atttttgctc
     1681 gcttcatcaa tgtaaggtat tccggtgaat attcaggctc ttctctcaga aaaagtccgt
     1741 caggccatga ttgcggcagg cgcgcctgcg gattgcgaac cgcaggttcg tcagtcagca
     1801 aaagttcagt tcggcgacta tcaggctaac ggcatgatgg cagttgctaa aaaactgggt
     1861 atggcaccgc gacaattagc agagcaggtg ctgactcatc tggatcttaa cggtatcgcc
     1921 agcaaagttg agatcgccgg tccaggcttt atcaacattt tccttgatcc ggcattcctg
     1981 gctgaacatg ttcagcaggc gctggcgtcc gatcgtctcg gtgtttctac gccagaaaaa
     2041 cagaccattg tggttgacta ctctgcgcca aacgtggcga aagagatgca tgtcggtcac
     2101 ctgcgctcta ccattattgg tgacgcagca gtgcgtactc tggagttcct cggtcacaaa
     2161 gtgattcgcg caaaccacgt cggcgactgg ggcactcagt tcggtatgct gattgcatgg
     2221 ctggaaaagc agcagcagga aaacgccggt gaaatggagc tggctgacct tgaaggtttc
     2281 taccgcgatg cgaaaaagca ttacgacgaa gatgaagagt tcgccgagcg tgcacgtaac
     2341 tacgtggtaa aactgcaaag cggtgacgaa tatttccgcg agatgtggcg caaactggtc
     2401 gacatcacca tgacgcagaa ccagatcact tacgatcgtc tcaacgtgac gctgacccgt
     2461 gatgacgtga tgggcgaaag cctctacaac ccaatgctgc caggaattgt ggcggatctc
     2521 aaagccaaag gtctggcagt agaaagcgaa ggggcgaccg tcgtattcct tgatgagttt
     2581 aaaaacaagg aaggcgaacc gatgggcgtg atcattcaga agaaagatgg cggctatctc
     2641 tacaccacca ctgatatcgc ctgtgcgaaa tatcgttatg aatcactgca tgccgatcgc
     2701 gtgctgtatt acatcgactc ccgtcagcat caacacctaa tgcaggcatg ggcgatcgtc
     2761 cgtaaagcag gctatgtacc ggaatccgta ccgctggaac accacatgtt cggcatgatg
     2821 ctgggtaaag acggcaaacc gttcaaaacc cgcgcgggtg gtacagtgaa actggccgat
     2881 ctgctggatg aagccctgga acgtgcacgc cgtctggtgg cagaaaagaa cccggatatg
     2941 ccagctaacg agctggaaaa actggctaac gcggttggta ttggtgcggt gaaatatgcg
     3001 gatctctcca aaaaccgcac cacggactac atcttcgact gggacaacat gctggcgttt
     3061 gagggtaata ccgcgccata catgcagtat gcttacacgc gtgtattgtc cgtgttccgt
     3121 aaggcagaaa ttaacgaaga gcaactggct gcagctccgg taatcatccg tgaagatcgt
     3181 gaagcgcaac tggcagctcg cctgctgcag tttgaagaaa ccctcaccgt ggttgcccgt
     3241 gaaggcacgc cgcatgtgat gtgtgcttac ctgtacgatc tggctggtct gttctctggc
     3301 ttctacgagc actgcccgat cctcagcgca gaaaacgaag aagtgcgtaa cagccgtctg
     3361 aaactggcac aactgacagc gaagacgcta aagctgggtc tggatacgct gggtattgag
     3421 acagtagagc gtatgtaatc gatttttcgt gagagtaaag cctgatcagg gttacctgat
     3481 caggcttttt tattgccatc taaatgtatt ctgaaaatgg acatgccatt gttttctgac
     3541 tgttggataa gaggccagaa gcgtaatatc cggccccagg gaaacgataa cggttgaatt
     3601 taaggaatac cgcagtgttt aaatttcttg tattaacatt aggcattatc tcttgccagg
     3661 cttacgcaga agatacggtt atagtaaacg accatgacat ttcagccatc aaagattgtt
     3721 ggcaaaaaaa ttcagatgat gatactgaca ttaacgtgat caaatcatgc ctgcgacaag
     3781 aatacaatct cgtcgatgcg caattaaata aagcctatgg tgaagcttat cgttatatag
     3841 aacaagtgcc acgcacaggt gtaaaaaaac ctgataccga acaacttaac ttgcttaaaa
     3901 aatcacagcg agcctggctg gattttaggg acaaagaatg tgaattaatc ctttcaaatg
     3961 aggacgttca ggatttaagt gacccttatt ctgaatcaga atggctctca tgtatgatca
     4021 tacagaccaa tacgcgaact cgccagttgc agctataccg taactctgaa gatttttatc
     4081 caagcccttt gacaagagga taattcacat ctttttggca tgttttgttg caagctattc
     4141 ctgataaata attgcaacaa gacatcgagc ctttttcact gagttattaa acatactcgc
     4201 gagcgcgtaa tttttttgtc cttcagcgat aattcacaat cacttcatta cgttgtacct
     4261 ttagcggtgg aattaaccgc ccaccgcctg gcacttccca gataaatcgc aacggttctg
     4321 ctgccgctat accggaaaag gccacggtgg ttccgctctg cccctctaat tcgacacaac
     4381 gagattgcga acacaagcga acccgcagtc ctgaaggtgt cgggccgata agctgataac
     4441 gccacgctac cagcgtcatc aatcctgaag caggttgtcg cgtagaaaga ggcgcagacg
     4501 acatcgactc accgcgatga tttagcgtaa ttcctacact acttgcctgc cacatcccct
     4561 ccccggcggc ttgcaccagc agcggaaaca ataatattgc taataaggtt ctcattattt
     4621 gccgccaatt gtcgccgtca tgcggatatg tcggttatca gacagttcca gattcgacag
     4681 gaccactaac tgcggcaagc tgcggcgcag gaagcgagac aataatggtc gcagcgcgtg
     4741 gttcaccaac aatactggcg gcgcacccag catctcctga cgggatagcg cttcctgagt
     4801 ttgcgccagt aaacgatccg ccagccctgg ctccagtcct cccccgccct gcagcgcctg
     4861 tagtaacaaa cgttccagcg gtgtatcgag gccaataaca tggacttcat ctttgccagg
     4921 aaaccactgc tgggtaatcg cccgtcccaa cgccacgcgc acgacggcgg ttaattcatg
     4981 tggatcgctt tggatgggcg catgttccgc cagcgtttcg agaatggtgc gcatatcgcg
     5041 aatcggtact ttttcatcga ggagattttg cagcacttta tgcagtgtgg tgagcgtgac
     5101 gacgccagga acgagatctt ccgtcagctt tggcatctcc tgggcgacgc gatccaacag
     5161 ctgttgcgcc tcctgacgac caaacagctc tgcggcatgc tggctaatga ggtggttaag
     5221 atgcgttgct accaccgtgc tggcctcaac cactgtgtac ccctgaatct gcgcctgttc
     5281 ttttagcgca ctttcaatcc agatagcatt caggccaaat gccggatcga cggtcgcctc
     5341 accaggtaac gtcccggcag cggttccagg gttaatcgcc agccagcgcc ccggataagc
     5401 atcaccactg ccaatctcca cgcctttcat caaaatgcga tagcgggcag gttgcagatc
     5461 catattgtcg cgaatgtgca ccactggcgg cagaaatccc atctcctggg caaatttctt
     5521 gcggatactg cgtatacggc ccaacaactc accatcctgc tggaaatcga ccatcgggat
     5581 cagtcgataa cccacttcca ttcccagaga atcttccagt tgtacatcgt tccacgtcgc
     5641 ttcgacaacg gtattattct ctgccatttt taccggtttg ggttcgacag gcgctttttg
     5701 ttcgcgtccg cgtatccacc aggccagccc cagcaatccg gcagtgaaca gcaaaaatac
     5761 caggttcggc attccaggca ccaggccgag taaaccgagc acggcggcgc ttaacaacat
     5821 aacgcttggg ttactgaaaa gctgattcac catctgctcg ccaacatcct gatcggtgct
     5881 gacacgcgta acgatgaccc ccgcggcggt agaaatcacc agcgccggaa tttgtgccac
     5941 cagaccgtcg ccaatggtca atagtgtata actttccgcc gcgtgtccca tgctcatgcc
     6001 atgttgcagc acgccgacca gcaacccgcc gacaacgtta atgaccatga tgaggatccc
     6061 ggcgatggca tcgccgcgaa caaacttact tgccccgtcc attgagccgt aaaaatcggc
     6121 ttcctgagtc acttcggagc ggcgtttttt cgcctcatct tcaccaatca atccagcgtt
     6181 aaggtcggcg tcaatcgcca tctgcttacc cggcatacca tcgagaacaa agcgcgcacc
     6241 cacttctgcg atacgcccgg cacctttggt aatgaccata aagttgatga tcacgagaat
     6301 gacaaacact acgataccga tagcgaaatt gccaccaacg aggaagtgac cgaatgcttc
     6361 gaccaccttc ccagccgccg ccgcgccggt atgcccttcc atcaaaatga tacgggttga
     6421 agccacgtta agtgccagac gcaacaacgt agtaaacaac agaatggtcg gaaacgcagc
     6481 aaactcaagc gtgcgctggg taaacatcgc caccagcaac accatgatcg acaaggcaat
     6541 attgaaggta aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca tcatcgacaa
     6601 gatcaacagg atcaaaatcg gtccggcaag gatctgccat tgtgtcgatt tcaggtttgc
     6661 gggcaggcgc agcatcgcgg ccagattact catgggtcgg tttctcgtta ataaaatcca
     6721 gggcttccgg caccggaaga tgagtaggtt gtacagggcg ctgtccacca gccagacgcc
     6781 agcgtttcag ttgccagacc caggccagca cttccgccac cgcggcgtac agttgacccg
     6841 ggatttgttg accaatctcc gcatgtcgat acagcgctcg cgccagcggc ggcgcttcaa
     6901 gcgtcgggac gttattttca gcgccaattt cacgaatgcg cagcgcgacc agccctgcac
     6961 ctttagcgac cactttcggt gcgctcattt tgttttcgtc atactgcaac gctaccgaat
     7021 agtgggtcgg gttattgaca atgacatccg ctttcggcac atcggccatc atccgacgcc
     7081 gtgcagcagc tcgctgcatc tgacggatcc gccctttaac atgagggtca ccttcgcttt
     7141 gtttgaactc atcacgaata tcctgccgtg acatacgcag ctttttcagg tggctgaata
     7201 tttggaaaaa gacgtcaaat cccaccattg gaatgacacc aagcaccacc agcagtgcgc
     7261 atagccctac caaatccatc gcattaccca tggcggtaat cggagactcg gccatcaagc
     7321 gcatcatctg cggccagtga tgccagagaa aaaaccccgt cacgctgcca accaggatgg
     7381 ttttcaaaat tgctttaagc aactccgcgc cagtctgagc cgagaacatc cgtttaatgc
     7441 ccggtagcgg gttgagtttg gaaaacttcg gctgcaagga tttgccgcta aataccagcc
     7501 ctcccagcat gaccggagaa atgagcgcca ccagcaccac gccgctaatc agcggcagca
     7561 gcgccagcat ggcttctctg atcagcagaa taatctgccc gaggatcaga ttcgggtcat
     7621 tgataatact gtgatcaaaa tgcagcccag cggagagcat gcccgacaat cgacgggcca
     7681 gcgacacacc gccaaaccag ataacactaa cgcccaccag caaaatcagc agtgaggtca
     7741 gttcacggga acgcgggatt tgcccctctt cccgcgcttt ttctagtcgg tgaggtgtgg
     7801 gggcttctgt tttgtcgtcg ctctcgtcag acacgtcgcc aatcctggaa agagtattaa
     7861 gcgtgaatga tgccagagcg caaagcgttc aatggtttga gtaaggggca aaacaggcgg
     7921 gatttagggc ttttgctgcc acacatcaag catagtgtgc gtttgtcgga tgcggcgtca
     7981 tcgccttatc agaccgcctg atatgacgtg gtcacgccac atcaggcaat acaaatcaaa
     8041 atccaagact atccaacaaa tcgtccacct gatcctgact ggctaccaca ccggctttgc
     8101 tggtatcgac ctgaggtcca ttaagcaaac tctggttttc acgttttgga cgcgactcct
     8161 gttccgggat gttttccaac agcaccatca gcaactggcg ttcgatctcc tgaatgacat
     8221 ccatcatccg cttaatgacc tgcccggtga gatcctgaaa atcctgcgcc atcatgattt
     8281 ccagcagttg cgcgttagta aagctggtat gcgcgggtac atctgccaga aattgtcgtg
     8341 tatctgttac cagttcacgg gcgtcggcaa ggtcaatcgg atcggcaaac cagtcatccc
     8401 aacgttgggt taacgctttt gctgatttct ccatttgatc ctgatgcggt tgtgacgcct
     8461 caacactgtt cagcgcccgc tccgcagcct gggcggtcat ctgcacaaca tagtacaaac
     8521 gatcgcgcgc atcggggatg gcttccgccg cttcggcaat ggcctgatcc agccccagtt
     8581 cccgcaaact gtcgcgcagc atacgcgtca ggctgccgat gcgcgcaatg atatcgccag
     8641 ctgaatgctc gtcagcaggt ttgattgatg gttgcatcat agtcgcatcc tcacatgccc
     8701 agtttctcaa agattttgtt gagtttttcc tccagcgtcg cggcggtaaa tggcttcacc
     8761 acatagccac tggcccccgc ttgcgccgca gcaatgatgt tctctttctt cgcttctgca
     8821 gtcaccatta acactggcaa tgccgacatc gcgccatccg cacgaattgt tttcagcaat
     8881 tccaggccat ccatattggg catgttccag tcggagataa caaatccata accgcctgcc
     8941 tgcaacttat tgagagcgtc gacgccatct tccgcttcct caacattatt gaatcccagc
     9001 tctttcagca ggttacgcac tatgcgtcgc atggtggaaa agtcatccac aaccaaaaat
     9061 ttaagttctt tatccgccat ttcacactcc tgatttaaat acgtatcgcc tgtccggcac
     9121 taatttttgc caacatttgc tggcttacct ggctaagatc gaccacttcg cagacaccac
     9181 ccatattgat ggcctcgcgc ggcatgccga acaccacgca acttgcttcg ttttgcgcaa
     9241 gggtccatgc ccccgcctga cgcatcgcca acattcccgc cgcgccgtcg ttgcccatac
     9301 cggtcaggat caccccaacc gcattacgcc ccgcctgttt ggcgacagaa tggaacaaca
     9361 catctaccga aggccgatga cggttaaccg ccgggccatc gtgaattttg atttggtaat
     9421 ttgcgccact acgcgacagc tccatatgcc gatcgcccgg cgcgatatag gcatgtcccg
     9481 gcaaaacacg ttctccgtct tcggcttctt taaccccgat ctgacaaagc ttattaagtc
     9541 tgtcggcaaa agagcgggtg aaaccaggcg gcatatgctg ggtaattaac agtgccgggc
     9601 tggaaagcgg caacggttgc agtacgtgac gaattgcctc agttccaccc gttgaagcac
     9661 caatcgcaat cagtttttca gaactcaaca acggccctgc cttcagcgtt gtcggtgccg
     9721 acaatggctt atgtgctgca aggctcgcct ttgctgctgt acgcaccttt tcagcaatca
     9781 tttcgctata cgccagcatt ccttcgcgaa tacccagttg cggtttggtg acaaaatcta
     9841 tcgcccccag ctccagcgcg cgcagcgtga cttctgaccc tttgccggtc aaggaagaaa
     9901 ccatcacaac gggcattgga cgcaaacgca ttaatttttc gaggaaatcc agtccgtcca
     9961 tccgcggcat ttcaacatcc agcgtcagca catcgggatt gaatttctta atcaagtcac
    10021 gcgcgaccag cggatcaggc gcggtcgcca ccatttccat gtcgctatgg ctgttgatga
    10081 tttctgtcat gatctggcgc atcagtgccg aatcatcgac agataacacc ctgattttgc
    10141 tcatcgttaa tccttactta gcgcatacac cgtctgacca cgcagcgtga agcggcgctc
    10201 aaggtggcta aagttttcag agtgacccgc aaacagcaat ccgtcgggtt taaggagcgg
    10261 aacaaagcgg cgcaaaatct cctgctgggt attttgatcg aagtagatca tgacgttacg
    10321 acagaagatc gcatcaaacg gccccggcac ggtgtactgt ttcgccagta gattcagcgg
    10381 ggcaaaatca acatagttcg ccagctcctg acgcacgcgt accagccctt catgcggtcc
    10441 cgtccctcgc atgaaatacc gttgcagttg ctgcggcgtc aggtttttca actcttcatg
    10501 gcgataaata ccgcttctgg ctttttccag cacttcggtg tcgatatcac tggcaaacac
    10561 tttccagcgt ccgggcgcgg tgcccaatgt gtcagccagc gtcatcgcaa tgctgtacgg
    10621 ctcttcgccg gtcgaagccg ccgcgctcca tacgcgatac tcgccagaac ggcgacgtgc
    10681 gtgatccgcg agcagaggga aatggtgtgc ctcacggaaa aatgccgtca gattcgtggt
    10741 cagcgaattg ataaacgcct gccactcacc gctgtgctga ttagattcca gcaagttcag
    10801 ataatgaccg aaatccgtca gtcccagcga acgcaaacga cgaaccagtc ggttgtaaac
    10861 catgtcgcgt ttatggtcag ccagaacgat cccggctcgt tgatagatca attgacttat
    10921 ccgccgaaaa tgcgcgtcgg aaagcgccag gcgctcggtc atctgtaaca ataaagacgt
    10981 ttgcccacag ggcagagatg aagtcatagc gccttctcaa tcacttcagg ataccactgg
    11041 cgcaatttgt aactgcgccg actcatgtcg tgctacttca tgttcttcaa gggtaaatac
    11101 cgccacacgc gacgaaagat ggtcggcctg attcgccagt tgttccgttg ccaccgccgc
    11161 ttcttctacc agcgaggcgt tctgctgagt cacctgatcc atctggctga cagcctgtgc
    11221 aacctgctca atcccccgct gttgttcttc cgacgcagag gcaatttctc ccataatgtc
    11281 gttcacgcgg gtcaccgaac tgacaatatc gatcatggtc gcggcggcgt tattcaccag
    11341 tttcgaaccc tgctggacac gattgactga ctcttcgatc agccctttga tctcttttgc
    11401 tgcctgcgcg ctacggctgg caagattgcg cacttcacct gccactaccg caaaaccacg
    11461 cccctgctct ccggcgcgag ccgcttccac tgccgcattc agggccagaa tattggtctg
    11521 gaaagcaatt ccgtcgataa cgctgataat gtcgccaatt ttttgcgagc tggtggcgat
    11581 ctcctgcatg gtgtgagtca tggtactgac ctgaacaccg cccgcctgcg ccgttgtcgc
    11641 ggcatttttt gccagttccg acgcctgtcg tgcgttatcg gcgttttgcc ctaccgtggc
    11701 ggttaattgc tccatactgg cggccgtttg tgccagcgat gccgcctgct gttcggtacg
    11761 acttgagaga tcgttattgc ctgcgacaat ctccgcgata ccaatgtgca tctcctggct
    11821 tcccttacgc acatcactta ccgtcccacg caaagcctgc tgcatggtct tcagactggc
    11881 aaaaatggcg gtgatctcat tacgaccata taccgcaatc ggacgcgcca gattacccgc
    11941 agcaatgctg tcaaaatggc taccgataat ggccagtggt tgaacaatca tcttgcgcgt
    12001 ccaccacagc gcactgctga tgtagatcgc cgcaacaata atcatgctga taaacaccag
    12061 cgccgaaatc tgatagttac gctggctttg cgcactggcg gcttccagca catggttgat
    12121 ctccagctgc caggcctcaa agttaacgtc aaacgcattc tgtgattcct gcaccggcgc
    12181 agtgaggaaa tccgaaagtt gattactttc aagccaggtc gcctgatgtt cgagatcgtt
    12241 gtgccagcgg gcaaaacttt tctccgtctc tttttgcaac gccctgacgt gctcgttgcc
    12301 cgcagtcatc gccataaaac ttttaaacag cgtggtggat tgcgtcagac tggcgcgcgc
    12361 cgtcgtcatc aacgttttaa tgtcatccgc cggatagcta agcgccgtca gagtgcccgc
    12421 tttgttcagc gcggtactgg cctgtaacat taccgcccga gtttgcgcta atgccgcacg
    12481 ttgctgattg ctctgctcga cctgattcag tcgttgcaaa tcgtcgcgaa atgcccaaaa
    12541 agacatgccg ttactgccaa tctgcaagat cccgcagaga atcaaaatta aaaacagcgt
    12601 ggtcgaaatt cgaatacgat taaacatcaa cgctccccat caggcggcaa tgaccgcgtt
    12661 agtaaatact cgtcaaaatg tttcccagtt tggatcttgt tcagcaattc gcagtcgtgg
    12721 ctgtgccggt ggttgctcac tggcaggacg ggatggtgtt tgcggtttat tggtgagtgg
    12781 gctggctgcc agacggaacg cggaaaccgc ttgcgttaaa cgactcgcct gttcttccag
    12841 cgcagcggcg gcggcagctg attcctgcac cagcgatgcg ttctgttgcg tgacgcgatc
    12901 catttccgaa accgccaatg cgacttgatc gatgccacgg ctctgttcat ccgatgccga
    12961 tgcaatctcg cccataatgt cagtcacgcg agtgacagca ttgacgatat tgttcattgt
    13021 ttccccggcg ctttcgacca gcaccgaacc ggtatcaacg cgtgagacgg agtcttcaat
    13081 gagggctttg atctcttttg ccgcctgggc gctacgactg gcaagattac gcacttcacc
    13141 cgccaccacg gcaaaaccac ggccctgttc acccgcacgc gcggcttcaa ccgcggcatt
    13201 cagcgcgagg atattagtct ggaaggcaat accgtcgata acgctgataa tgtcggcaat
    13261 tttcttcgaa ctatcggcga tctcatgcat cgttttcact acgccatcca ccactttgcc
    13321 gccgtgctgg gcggtgtcgg aggcactttg cgccagttgc gaggcctggc gggcgttatc
    13381 ggcgttttgc ttcactgtcg cggtgagctg ctccatgctg gcggcagttt cttccagcgc
    13441 ggatgcctgc tgttcagtac gggaggaaag atcggtgttg cccgccgcaa tttcacgggt
    13501 accggcatag atggcatctg aaccttcgcg gacatgagtg acggtgtcag tcaaagagcg
    13561 ttgcatatgt gaaacgctct gcgccaggtc gcccatttca ctgcgcccgt caatggtcag
    13621 ggtattcgcc aggttaccac cggcgatttc gcgaatgtga gcaataattt ttgccagcgg
    13681 agtaagcaac atacggcgaa tgccgtacca cgccaccagc agaatcaata ccaccaccag
    13741 cgcgataacc gccagttgcc actgggcaaa tcggtaatca tctgcgttgt cagtgacgat
    13801 atcgcgatac agtttttcac tgctgagggc gtactgagca aacgcttcgc ccattgcatt
    13861 ttgcattccc tgggttggct gagcgaaata agctccagta ttgccataat ctagataatc
    13921 aatcagttca gttaacgctg tgtaatagtt tttatatttt tcatcaatat tacgactggt
    13981 agcgaccatt tcaggtaacg gtgccatgct tttgaatttt ttataatgcg tcgctgcctg
    14041 cgccaatgtt ttcctggcgc tatcgagcaa ttcaactttg gcgttacttt gttgattgga
    14101 ggaatccatc atcatccgta ccgctgaacg actcaggtta atgcgcgttt gcagcattaa
    14161 atcccaggtt gacgtcagct cgccctgctg ttcccgtaat tgattggaaa ccacaaagct
    14221 cttctggcta tggtgaaggg aagaaaaaaa cagactgccg gaaataagct gtaacagtgc
    14281 gaataccccc agcaccatta ccaacagcgt gactacgcgg atacggttaa tcataaggca
    14341 ccttcctgaa aacaagttga tctcgttatc ggcaaggagg ggggaaactt tattgctgat
    14401 gccacccgcc gcgaaattga aataaaaaac ccgatgcgca gatcatcggg ttcatttcaa
    14461 ttgaggaaat cgggagaatt acgccacttc tgacgccgcg ctatctaaca gcgccatctc
    14521 ttcgctgttc agcagttttt cgatgttcac cagaatcaac atccggtcgc ccagtgcgcc
    14581 cagtccagtg agatattctg ttgaaagcgt cacggcaaat tccggtgccg gacgaatttg
    14641 ctccgccgtc aatgaaagca cgtctgagac gccgtcaacc acgatgccga ccacccgctg
    14701 tccgagattc aggacgataa ctaccgtgtt gtcgttatag tccacatcca cctggctgaa
    14761 cttaattcgt aagtcaacaa tcggcacaat aaccccgcgc agattcgtga cgcctttgat
    14821 aaacgctggc gtgttcgcaa tccgtgttac ctgatcgtag acacggatct cctgcacttt
    14881 caggatatca ataccgtact cttcatcacc aagggtaaat accagaaatt cctggcctga
    14941 cggctcgctg gccagctttg ttacattcgt cataccggtc atattgttac ctttttactc
    15001 attcaggcgg cggtgttcgc catacgttgt tcgcggttta tcgcctgcaa ggcggagaca
    15061 tcaacaatca gtgccacgct gccgtcgcca agaatggtcg cagcagaaat gccggggact
    15121 ttgcgatagt tactttcaag gtttttaacc acaacctggt gctgaccaat taattgatcc
    15181 accagcaagg cgtagcggcg accgccactt tgtaagatca ccacaattcc ctgggtggct
    15241 tcggttttcg cgcccgcgac gttgaacact ttccacagtt cgacgatggg cagatattca
    15301 ccccgcactt ccagcacccg ctcgccgccg gccagtggat gcagatcggc ttcacggggt
    15361 tgcagtgatt ccataacagc attcagcggc agaatgaaaa cttcatccgc aacgcgtacg
    15421 gacatgccgt cgaggatggc cagcgtcagc ggcagtaaaa tgcggatcgt agtgccagta
    15481 ccctgcttcg actggatttc gacatgaccg cccatctcct ggatattacg tttaacgacg
    15541 tccatgccga cgccgcgccc ggagacgtcg gtgacctgct ctgccgtgga gaagcctggt
    15601 gcaaatatca gcatcgcgac ttcgtcgtcg ctcatgtttt cgctgacagt caaaccttgc
    15661 gaggccgctt ttgccagaat tcgctcacgg tttagccccg ccccatcgtc ggtcacttca
    15721 atgcaaatgt tgccgccctg atgttcggca gacagaatta aatttccgac gctgttttta
    15781 cctgcggcga gccgtttttc tggcagttca ataccgtgat cgaggctatt gcgtaccagg
    15841 tgggtcagcg ggtcgataat gcgttctatc aggctcttgt cgagctcggt ggaactgccc
    15901 accagcgtca gttctacctg cttgccgagt tttcccgcca gatcacgcac cagccgggga
    15961 tagcgactaa agacatattc catcggcatc atgcgaatcg acatcactga ttcctgcaaa
    16021 tcacgggcgt tacgttgtaa ctgccccatg ctggtaatca aatcaccatg attaaccggg
    16081 tccagttcgc tggaacgctg ggcaagcatg gactgggtga taatcagctc gccgacgagg
    16141 ttaattaatt gatcaacctt ttctaccgct acacggatgc tggtggattc actgctgcgc
    16201 gtcgtttttt cccgctccac gcgaccggtt ggcgcttgtt cggctgccag tttaagcact
    16261 ggtggggtgg atatttttgg cgagacttct actgtttcaa aggtaatctg atcggcttca
    16321 atcacaaaac agagtaccgc tgtgatgtca tcttcggcga tatcgcccgg taatattgcc
    16381 gagagagaat ccgccccttt caccacgtca gttaacgttg tcagatgccc cagctcttct
    16441 tccagcaggt cgacttcgcc ggccttcagg cgcgaaagga taattcgtcg cggcgactga
    16501 ctgcgactct gctcatcttg cggttcactt ttggcaacca cacttaatcg ggtcactgcg
    16561 gatggcgttt cgcctttcgc ttctaatgcc agttgacgca aggcctggca gatataatcg
    16621 aagctggcgg catccggctc ttgcgactgt ttataagcgt cgagctgttc ttgcatgatg
    16681 tccttcgttt ccaaaaacag attgataatg tcggtgttga gttgcatctc acctcgtctg
    16741 gcttcatcga gcaggttttc catcagatgc gtggtttcct gcaaaacgct gaagccaaaa
    16801 gttcctgccc ctcctttgat cgagtgggca gcccgaaaga tggcattcaa ttgttcggca
    16861 tctggcgctt ccggctgcaa aaccagcaga tgctgctcca tatcagccaa cagttcgtcc
    16921 gcttcatcaa aaaatgtctg ataaaaatcg cttatatcca tgctcacgct gtcacctcgg
    16981 ttcggctgat ggcattgtgg gaacactgac ctgtggtgca acctcaggtt tttccagggc
    17041 gcttactggc tcattctggc tttcggcgtt ttcatgcaaa atggcctgtt cggcttgttt
    17101 gttcagtacc agcaggctga tgcgacggtt gacggcatca tcaggtccgc gatcgcttaa
    17161 gcgcatcgtt gccgccatgc cgacgacacg taacactttg ccgctatcca accctccgac
    17221 catcagttcg cggcgggatg cattggcccg atcggcagaa agctcccagt tgctatatcc
    17281 tttctcaccg ctggcgtagg ggaaatcatc ggtatgacct gaaaggctaa tacggttggg
    17341 aataccgttc agtacaggcg caatggcacg cagaatgtcg cgcatatagg gttcgacatc
    17401 ggcactgccg gttctaaaca tcgggcgatt ctggctatcg atgatctgaa tacgtagacc
    17461 ttcctggacc agatcgattt tgagatgggg acgtaacgcc cgcagtttcg gatcggacga
    17521 gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcagc tcttcgatgt
    17581 tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg ccgggaattg
    17641 ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt ggagtccgga
    17701 agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc cacatcacca
    17761 gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac gatccatgtg
    17821 ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc gcttgattct
    17881 tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca catgctcttc
    17941 cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa actcaacggc
    18001 gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc actgcatcat
    18061 tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa taaatccgta
    18121 agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg cacccagctc
    18181 ggcggcagga cgatcggctg aacctaacgc gtgaacgacc cccattacag ccgcgacaat
    18241 accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga cttctgcctc
    18301 gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg tgttcatatg
    18361 accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc tatccgcgag
    18421 gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat cacgttccag
    18481 cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag ccagcagatc
    18541 catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg ccttcagcgt
    18601 gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg caccggcaat
    18661 aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca tcaaataacc
    18721 gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga catcatcctt
    18781 ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat tggggcaaaa
    18841 aaaagcagcg gtacgtcgtt accgctgctg gaatgttgcg cctcaccgta tcagttaaac
    18901 agcctgtact ctctgttcat ccagcagttg tgggataata tcggcaggat tctgggaaag
    18961 tttacgtctt tttactgccc gggatggcgg ttgacattag ctgcaggcaa agctgccaac
    19021 aggctggtga gcgtgggtaa taaaattgcc gccgcagcag ttgcagctgg aaagttgcag
    19081 taatccactt tcaacaaacc gcaccaatgt ccaggcacgg gttaatgcca gcagtggtcc
    19141 ttcttctgct tgtgggcact gttcaaggta taaacggtag gctttgatca ccgcatcgac
    19201 gccattacac aaaccggttt tcagtaaaaa ctgccatgca ttacagaaca tcgaagcatg
    19261 aacgttttgt tcccaggtca taaaccagtc ggttgagaat ggcagcatgc ctttcggcgg
    19321 tgggcttccg cgcagttctt tataaagttt tatcaggcgt ccgcgactta actgtgtttc
    19381 gctttccagc atctgcaaac gagctcccag ggtgatcaat tccattgcca gctgaatatc
    19441 ccgcgcttcc tgaacaatgc ttttttcact catgatcagg cccttttctt gcgcagcgct
    19501 tcttcaggct gattaacatc attcagcaag cgtgttgaga gcatgatgcc ggtatgaatt
    19561 tgctggagat cgtcaacgcg ggaatcttgc gtcaactgag taatcgtctg gtggctgtca
    19621 aaacggaagt gacaaaccag ttgattggtt tctgccagct taaccatttg cggaagagtc
    19681 agtgccgcta acgttgtcgc catttcttca tttatgccga gacgaaacat agcggacgct
    19741 ttgtcctgaa caatcaaacg ctgtgcaagt agtaaatatg acaagttgat gtcataaatg
    19801 tgtttcagca actcggaggt atgcattatt cccacccaga ataaccaact ttatttttat
    19861 gcggtttcac cgcaccccgt gatgtcgccg ggaagccccg gtaaaaaata attagcatta
    19921 gaatagttgc gataagctgc aataagcaga accacctttt tggtttaata tgtccttaca
    19981 aatagaaatg ggtctttaca cttatctaag atttttccta aatcgacgca actgtactcg
    20041 tcactacacg cacatacaac ggaggggggc tgcgattttc aataatgtgt gatgcagatc
    20101 acacaaaaca ctcaattact taacataaat gtataagtca tacttttgtt ttgggtgtat
    20161 ttcaatctgt taaaaagttt ttcgctacgc tagcaagcaa aaatgaaaca ggaataatcg
    20221 aaatgggatg ttgcgcacag ttaaaataac tcaccgtaaa taatcatctg ctataaataa
    20281 tcactttcat gcaataccag ataagctatt tttaaacaga cacttaccgc acaacaaact
    20341 aattaaatac aaagaaaggc aacaatcaac ttaatgttaa tgaaatgttt tatatatatt
    20401 aaccacttgt ttaatgcgga gaatcagtac actattttct tataattaca tttgaaatta
    20461 tatgatcacc gggagtgatt gattaattgt ctttactaat aatcagacta atatttacct
    20521 gtttgaccga gttgggattg cgtcgtttct ccattaggag taaagcttta atgtcacctg
    20581 aagttcacag aataaggaac aggttatgag ctatagcaat attcttgtcg ctgttgcggt
    20641 tacaccggaa agtcagcaac tgctggcaaa agcggtatct atcgccaggc cagtaaaggg
    20701 acacatcagt ttaattactc tcgcttccga cccggaaatg tacaatcaat tagccgcgcc
    20761 gatgcttgaa gatttacgca gcgtgatgca agaagaaacg caaagctttc ttgataagtt
    20821 aattcaggat gcggggtatc ccgttgacaa gacgtttatt gcctacggtg agttaagcga
    20881 acatattctg gaagtatgtc acaaacatca tttcgatttg gtgatttgcg gtaatcacaa
    20941 tcacagtttc ttttcgcgag catcctgctc ggcgaaaaga gtgattgcct caagtgaggt
    21001 cgatgtgctg ttagttccac ttacgggtga ttaaccgctc ctacgcaagc tttggaaagg
    21061 tagcaacttt atcgcgctgc tggctttccg cgcttcgcgg aactatctgc tttaggtcgc
    21121 taatgaagca ctcctgccag tggttaatat cgtttttcac gataacgtcc agcatttctg
    21181 catgacggga aatacgttcc gccagcgaca tagtcaatgc acgatccagc gcagctgcaa
    21241 cttcgtcacg atcgtagggg ttaacaatta acgccgacgt taactcgttt gccgctcccg
    21301 caaattgcga aagaacaaga acgcccgggt tggctgggtc ctgagcagca acatactctt
    21361 ttgctaccag gttcatcccg tcacgcagtg gcgtcactaa gcccacgtca gagtagcgga
    21421 atattttcat cagtaattta cggtcaaaat gctgattcaa ataataaagc ggcgtccagc
    21481 ctaattgccc gtatttacca ttaattcgtc cagcttcatt ttcgagctga tgacgaatat
    21541 cctgataggc ttgcacatca ccacgcgacg ttggtgcaat ctgggtataa cgaattttac
    21601 catgatgctg cggatatttt tccagcaacg cttcataggc gagaaaacgc tctggcaaac
    21661 ctttggaata atccagccgt tcgacagaaa agatattttg tacgtttttc agttccgctt
    21721 taagttgcgc cagttttggc ggcagtggcc cggcagcctg tttggctatt tctttcggtt
    21781 caatgccgat cgggtagact tctgttcgaa atgctttgcc ccaggctgta tggctttttg
    21841 cgctacgtgt cgtgacgcgg gtcaggttag aaagacaatc caggaacgcc agacgatcgt
    21901 tttctgtctg gaaacccagc aaatcataat cacaaagctg ttcaagcaag gtgtcatatg
    21961 tcggcagcgc gttgaagatt tccggtgtcg ggaaaggaat atgcagaaag aaaccaatgc
    22021 gattattcac tccccgtttg cgtaattcat gcgcaaatgg caacaggtga taatcgtgga
    22081 tccagataat gtcatcgtct tgcaacagcg gcagtaattt atctgccagc aacgcattta
    22141 cgcgtagata gccgtcccag gcaggacgct gaaattgcac cagatcgagc cgataatgaa
    22201 aagcgggcca gagaacggca ttggagaatt ggttgtagta ttcgtcaagg tcctgttcgc
    22261 tgaggttaaa agaggcccac gtaatgttac cttttttcac cttttttagc ggctgatcct
    22321 cattccctgt ttcaccactc cagccaaacc acagtccgcc tgcggctttc agtgccccca
    22381 gtatgccaac ggcaaggcca ccggcactgg cggcgtgctc gtctggtggt gcaatccggt
    22441 tagatactac gactaaacga ctcatagtca tcactcctgt tattttctct tttttgttgt
    22501 aatgcggtgg ttatcatttc aagccagctc cagacatccg gcacacccgc cagtcgccat
    22561 gatgcctgag ttgcacctgt gccaattttt actgacattc cgcccagtcg gttaacgact
    22621 gcgaagccag attcatcggt taaatcatcg cccagaaata cgggcgttcg cccgataaag
    22681 ggagcttcct gcataaaagc tgcaattgcc tcacctttac tggtacctct cggtttgatc
    22741 tcgacaacac actttccctg ctgtaacgcc atttgtggcc agatctgagt aatacgttgc
    22801 gctaatgtca ttaatgcgtc ttcatgctgc ggagcctgac gataatgcag cgcaaaagcc
    22861 atccctttcg cctccagctc cgcgccggga tactgagcga tgactgtatg cagttgcacg
    22921 ctaatatcac gcgcaatcgc atccggcaga tgaacgatat gtgttttacc attgatgtca
    22981 cggcgctccg ccccatgcac gcccgctaac gggaagcgat aaggttttgc cagtgcgtca
    23041 agctccacca ttgagcgccc tgatatcaat gccaatgcac catcacttgc ggttgccagt
    23101 agctgtagtc cttgcagaat attgtcaggc acgacgacct gatcgggatg cggtttgatt
    23161 tccgccagcg ttccatcaag atcaaaaaac caggcatatt tcgcggatag ttcaggggtt
    23221 tcggttaacg gttctgtcac ccggctctcc tccttctctt ttcttattgt tttgcgcaga
    23281 acgcgcttta cagacatgta agtatagaca gtgtgacggg ggtcgccatt tgcaatgaag
    23341 aacagccaac cgttgtggcg gctggctgtg gtgggaaaaa acgttaaatt gttgtggaaa
    23401 aaagcatcag acagtgcgtt tcgctttttg cttgtaacgg tcgaagatca ccgctgccag
    23461 caggattaag ccgcgaacca cgtactgcgc gaaaggagaa atattaagca ggttcatggc
    23521 gttttccacg gtgcctaaaa ttaagatacc cgccaccaca tatgagattt ttccgatgcc
    23581 acctttcaga gaaacgccac ctaaaacgca ggcggagata acaataagct cataaccaat
    23641 cgacgtcatt ggctgcccac tagtcatacg tgaagccaga ataattccgg ctatcgcaga
    23701 taccaggcca gagagaacaa agataataat tttggtgcga acaaccggta cacccgccag
    23761 acgcgcagcc tcttcgttcc cgccaatcgc cagggtgtta cgaccaaagg tggttttatt
    23821 cagcaacaaa ccaaagataa tcagacacgc gacggtgagc cagattggcg caggcagacc
    23881 gaaccagtta gcgtaaccaa gggcaaagaa gctttcatct tcgataccga ccgctttacc
    23941 gtctgaaatg atgtacgcca gacctcgaac aatctgcatc gttgccagtg ttgtgatcag
    24001 agcatttatt ttcagtttgg cgataacaaa gccattgacc aggccacaga gaatgccaag
    24061 cagcaacccc gctgccacgc caatccacag gctttcagtc aggttgataa ccaccgccgt
    24121 ggtgacaccc gcacaggcaa ttacggaggc gacagaaagg tcaaagtcac cggaagcaag
    24181 gcagaacaac atgccacacg ccaccatccc cgacatggaa attgccaggc ccaacccttt
    24241 catattaatg aaggtggcaa aatttgggac aaaaatggca caggcgataa agagcaccgc
    24301 aaacaccacc agcatgccgt actgatccca gatacgcccg aagctgaatg acgacttagg
    24361 tgcgccagac cccgatgtag aaacagaaga catcatactc tccttactca ggcaactgcc
    24421 tggctgactt taggcatcgc aaggctcagt gcctgacgct catctgcctg ctcgtgtaac
    24481 aattcaccgg cgatttcacc ttcccgcatc accacaatcc ggtcggcaac gccgaggact
    24541 tcaggtaagt cgctggaggc aaacagcacc gccacaccct gcgccgccag cgcataaatc
    24601 acgttgtaaa tttcatgctt agcaccaaca tcaatgccgc gcgtaggttc atccagcaaa
    24661 atgaccttca tctcttccga taaccagcgg cccagaatgg ctttttgctg atttccgcct
    24721 gagagattca tgatcagttg ctcagcgccc ggcgttttga tgttgagcga acgaatgtgg
    24781 tgatcggcat tgttttcttc ccaaccgttg ttgattacac aaccgccgag cacatgttta
    24841 cgtctggcac tgatgttgat attgtcgcga acggagtgca cgggaataat gccttccgct
    24901 ttgcgatctt ccgggcagag catcatgcct gcggcaatgg cgtggctcgg tttacgaata
    24961 tcgatcggct gttggtcgat ataaacctga ccggcggtga tttgcgtccc gccaaacaag
    25021 cctttcatta attcgctacg ccccgctcct accagaccaa acagcccaac aatttcacca
    25081 ctgcgaaccg ccagacttat tggcgtacgc acgcctggtg ctttcacagc atcaagacgc
    25141 aggcgctcct cgccataact acgaggttgc cagccgtaga tatcgccaat gtcgcgcccg
    25201 accatcgcct gcaccagcgc gtcgtggtca acctgctgca tatcggtaaa ggttttgaca
    25261 taacgtccat ctttaaagac agtgatggca tcgctgaggg caaatatttc ttccatacgg
    25321 tgagaaacgt ataagatcac ccgcccctct tttcgcagtt cacgaataac gcggaaaaga
    25381 ttgtcgattt cgcgggcaga aagggagctg gttggctcat caaaggcgat aattttagcg
    25441 ttacgcgcca gcgccttggc gatttcaacc atctgccact gaccaatgga gagatatttc
    25501 agcggcgtgt ccgggtcaat atccatacca agatgtttaa gttgtaaacc cgcctcataa
    25561 ttcagcaatg agcgattcac aatgccgcct ttatgcggca gctggccgag atagatgttt
    25621 tccgcgacgg tcatttccgg cacgagatgc agttcctggt aaataatcgc cacacccgca
    25681 ttaagtgctg cggtcgtgtc ggaaaaggac atttcctgcc cattaatcac tacagaaccc
    25741 gtggttggcg catagttgcc gctgaggatt tttaagagag ttgattttcc tgcgccattt
    25801 tcacccatca acgcatgaac ctgaccggca tagcagtcaa aactaatatc cgtcagcgcc
    25861 ttaacgccgg gaaacgtttt accgatgccg cgaaatgaga gatacggggt agactgttgc
    25921 ataacgtctc cgtgaatcac tggtcgttca caccccctct gcatcatgca gaggggaatt
    25981 tttccggcaa attacttacc gcctaaacct tttttctcca gttcttcttt aaagttgtca
    26041 cgcgtgatca gtaccacgtc ggtaacttcg gtaaattttg gcggttcaac gtcttttgct
    26101 acccagttgt aaagcatttc gctggattta tagccatgta cgtccgggct tggcagcagg
    26161 gaaccgtaga agccggttgc ctgtgcttta gacagttcgc tcaccgcatc cacaccgtta
    26221 atgccaatgc cgatgatatc ggccgcttta aagccctgac cttccgtcgc gcgtacgccg
    26281 cccagcacgg tgctgtcgtt cataccgacg atcagccaat gtttaacttc cggatgttga
    26341 accagcattg agttggcagc gtcaaatgcc cccgggatgt cgttagattt ggtaggtacc
    26401 tgataaattt gtttttccgg gaatccggcc gctttcagcg catccataga tcccgtagta
    26461 cggcggcggg cggtatccag ttcgttggcg gtaatcgcca tcaccgcgct ttctttgaca
    26521 tcccagccac gtttctgcat ctctttatac agttcctggc cctgacgttc gccaatttta
    26581 gtcgccgcca tcatcaccag cggaacggta tccattggct tacctttggc gttaacaaac
    26641 tggtcatcca cggcaatgac tttcatatcg tagccacgcg ctttcgcgac gatggcagag
    26701 ccgagtttgg ggtccggagt acaaataacg aaaccttttg cgccactggc agccaggctg
    26761 tcgatcgcgt tcaatgtttt ttcgccatcc ggcacggcaa tcttaataac ctcaaaccct
    26821 aaatccttcc cggctttatc ggcaaacttc cattcggtct ggaaccacgg ctcttccggt
    26881 tgcttcacca gaaaaccgag cttcaggttc tccgccatag cggattgtga cataacggct
    26941 gccagaccaa tggctgccag ggctttagta aatttgtgca tggttctctc cagctttagt
    27001 gtcgttttgt gtagggcaaa aacgaatgac attcgttaaa ttaatcggaa aacaaagcat
    27061 taccttttaa ctaaaagata agtgaccgtg ttgacatagt tttagcgaga aattaattct
    27121 ccataggaga gcaatatcac atcgcagaat tacagtgaga acgtgcataa atttagcggg
    27181 aaaagacata agggaaagcc aatttgtcag acaaattgtc gaatgcacag cagattaatc
    27241 cataagatta gcctggaaat ccttgttgtc tttggtaccc atgcgggatg tcttcttttt
    27301 aaccagtcaa taggccgcat tacctggcgt tgagtttttg aaatggtgta ataaccgcaa
    27361 ctcaaagatg tggaaaatgc acgtcattca tttcgtcatt aattatcact gtgctcatta
    27421 attaacagaa cacgtataat gagagc
//
LOCUS       DAZGAN020000057        27325 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGAN020000057 DAZGAN020000000
VERSION     DAZGAN020000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.057"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            93..884
                     /gene="nanR"
                     /locus_tag="ACHKYO_RS13500"
                     /old_locus_tag="pgaptmp_002700"
     CDS             93..884
                     /gene="nanR"
                     /locus_tag="ACHKYO_RS13500"
                     /old_locus_tag="pgaptmp_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HGU4429522.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            1006..1899
                     /gene="nanA"
                     /locus_tag="ACHKYO_RS13505"
                     /old_locus_tag="pgaptmp_002701"
     CDS             1006..1899
                     /gene="nanA"
                     /locus_tag="ACHKYO_RS13505"
                     /old_locus_tag="pgaptmp_002701"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGU4429523.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            2009..3499
                     /gene="nanT"
                     /locus_tag="ACHKYO_RS13510"
                     /old_locus_tag="pgaptmp_002702"
     CDS             2009..3499
                     /gene="nanT"
                     /locus_tag="ACHKYO_RS13510"
                     /old_locus_tag="pgaptmp_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HGU4429524.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            3547..4236
                     /gene="nanE"
                     /locus_tag="ACHKYO_RS13515"
                     /old_locus_tag="pgaptmp_002703"
     CDS             3547..4236
                     /gene="nanE"
                     /locus_tag="ACHKYO_RS13515"
                     /old_locus_tag="pgaptmp_002703"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGU4429525.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            4233..5108
                     /gene="nanK"
                     /locus_tag="ACHKYO_RS13520"
                     /old_locus_tag="pgaptmp_002704"
     CDS             4233..5108
                     /gene="nanK"
                     /locus_tag="ACHKYO_RS13520"
                     /old_locus_tag="pgaptmp_002704"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HGU4429526.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            5105..5569
                     /gene="nanQ"
                     /locus_tag="ACHKYO_RS13525"
                     /old_locus_tag="pgaptmp_002705"
     CDS             5105..5569
                     /gene="nanQ"
                     /locus_tag="ACHKYO_RS13525"
                     /old_locus_tag="pgaptmp_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HGU4429527.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(5629..6756)
                     /gene="yhcG"
                     /locus_tag="ACHKYO_RS13530"
                     /old_locus_tag="pgaptmp_002706"
     CDS             complement(5629..6756)
                     /gene="yhcG"
                     /locus_tag="ACHKYO_RS13530"
                     /old_locus_tag="pgaptmp_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HGU4429528.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(6940..8358)
                     /gene="gltD"
                     /locus_tag="ACHKYO_RS13535"
                     /old_locus_tag="pgaptmp_002707"
     CDS             complement(6940..8358)
                     /gene="gltD"
                     /locus_tag="ACHKYO_RS13535"
                     /old_locus_tag="pgaptmp_002707"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HGU4429529.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(8371..12831)
                     /gene="gltB"
                     /locus_tag="ACHKYO_RS13540"
                     /old_locus_tag="pgaptmp_002708"
     CDS             complement(8371..12831)
                     /gene="gltB"
                     /locus_tag="ACHKYO_RS13540"
                     /old_locus_tag="pgaptmp_002708"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HGU4429530.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPHIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEALGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            13506..14435
                     /gene="yhcC"
                     /locus_tag="ACHKYO_RS13545"
                     /old_locus_tag="pgaptmp_002709"
     CDS             13506..14435
                     /gene="yhcC"
                     /locus_tag="ACHKYO_RS13545"
                     /old_locus_tag="pgaptmp_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HGU4429531.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFARYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            14531..16867
                     /gene="arcB"
                     /locus_tag="ACHKYO_RS13550"
                     /old_locus_tag="pgaptmp_002710"
     CDS             14531..16867
                     /gene="arcB"
                     /locus_tag="ACHKYO_RS13550"
                     /old_locus_tag="pgaptmp_002710"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HGU4429532.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGLVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            17097..17750
                     /gene="elbB"
                     /locus_tag="ACHKYO_RS13555"
                     /old_locus_tag="pgaptmp_002711"
     CDS             17097..17750
                     /gene="elbB"
                     /locus_tag="ACHKYO_RS13555"
                     /old_locus_tag="pgaptmp_002711"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HGU4429533.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            17747..18475
                     /gene="mtgA"
                     /locus_tag="ACHKYO_RS13560"
                     /old_locus_tag="pgaptmp_002712"
     CDS             17747..18475
                     /gene="mtgA"
                     /locus_tag="ACHKYO_RS13560"
                     /old_locus_tag="pgaptmp_002712"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HGU4429534.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(18472..19104)
                     /gene="yrbL"
                     /locus_tag="ACHKYO_RS13565"
                     /old_locus_tag="pgaptmp_002713"
     CDS             complement(18472..19104)
                     /gene="yrbL"
                     /locus_tag="ACHKYO_RS13565"
                     /old_locus_tag="pgaptmp_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HGU4429535.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSGEA"
     gene            complement(19317..19589)
                     /gene="npr"
                     /locus_tag="ACHKYO_RS13570"
                     /old_locus_tag="pgaptmp_002714"
     CDS             complement(19317..19589)
                     /gene="npr"
                     /locus_tag="ACHKYO_RS13570"
                     /old_locus_tag="pgaptmp_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HGU4429536.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(19586..20440)
                     /gene="rapZ"
                     /locus_tag="ACHKYO_RS13575"
                     /old_locus_tag="pgaptmp_002715"
     CDS             complement(19586..20440)
                     /gene="rapZ"
                     /locus_tag="ACHKYO_RS13575"
                     /old_locus_tag="pgaptmp_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGU4429537.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(20486..20977)
                     /gene="ptsN"
                     /locus_tag="ACHKYO_RS13580"
                     /old_locus_tag="pgaptmp_002716"
     CDS             complement(20486..20977)
                     /gene="ptsN"
                     /locus_tag="ACHKYO_RS13580"
                     /old_locus_tag="pgaptmp_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HGU4429538.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            complement(21095..21382)
                     /gene="hpf"
                     /locus_tag="ACHKYO_RS13585"
                     /old_locus_tag="pgaptmp_002717"
     CDS             complement(21095..21382)
                     /gene="hpf"
                     /locus_tag="ACHKYO_RS13585"
                     /old_locus_tag="pgaptmp_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HGU4429539.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(21405..22838)
                     /gene="rpoN"
                     /locus_tag="ACHKYO_RS13590"
                     /old_locus_tag="pgaptmp_002718"
     CDS             complement(21405..22838)
                     /gene="rpoN"
                     /locus_tag="ACHKYO_RS13590"
                     /old_locus_tag="pgaptmp_002718"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HGU4429540.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(22886..23611)
                     /gene="lptB"
                     /locus_tag="ACHKYO_RS13595"
                     /old_locus_tag="pgaptmp_002719"
     CDS             complement(22886..23611)
                     /gene="lptB"
                     /locus_tag="ACHKYO_RS13595"
                     /old_locus_tag="pgaptmp_002719"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HGU4429541.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(23618..24175)
                     /gene="lptA"
                     /locus_tag="ACHKYO_RS13600"
                     /old_locus_tag="pgaptmp_002720"
     CDS             complement(23618..24175)
                     /gene="lptA"
                     /locus_tag="ACHKYO_RS13600"
                     /old_locus_tag="pgaptmp_002720"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HGU4429542.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(24144..24719)
                     /gene="lptC"
                     /locus_tag="ACHKYO_RS13605"
                     /old_locus_tag="pgaptmp_002721"
     CDS             complement(24144..24719)
                     /gene="lptC"
                     /locus_tag="ACHKYO_RS13605"
                     /old_locus_tag="pgaptmp_002721"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HGU4429543.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(24716..25282)
                     /gene="kdsC"
                     /locus_tag="ACHKYO_RS13610"
                     /old_locus_tag="pgaptmp_002722"
     CDS             complement(24716..25282)
                     /gene="kdsC"
                     /locus_tag="ACHKYO_RS13610"
                     /old_locus_tag="pgaptmp_002722"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HGU4429544.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(25303..26289)
                     /gene="kdsD"
                     /locus_tag="ACHKYO_RS13615"
                     /old_locus_tag="pgaptmp_002723"
     CDS             complement(25303..26289)
                     /gene="kdsD"
                     /locus_tag="ACHKYO_RS13615"
                     /old_locus_tag="pgaptmp_002723"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HGU4429545.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(26303..27280)
                     /gene="yrbG"
                     /locus_tag="ACHKYO_RS13620"
                     /old_locus_tag="pgaptmp_002724"
     CDS             complement(26303..27280)
                     /gene="yrbG"
                     /locus_tag="ACHKYO_RS13620"
                     /old_locus_tag="pgaptmp_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HGU4429546.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
BASE COUNT         6298 a         7066 c         7316 g         6645 t
ORIGIN      
        1 tgaggcacaa agagaaaaac ctgccatttt cccctacttt caatcctgtg ataggatgtc
       61 actgatgatg ttaatcacac tgaccttaca gaatgggcct tatgaacgca tttgattcgc
      121 aaaccgaaga ttcttcacct gcaattggtc gcaacttgcg tagccgcccg ctggcgcgta
      181 aaaaactctc cgaaatggtg gaagaagagc tggaacagat gatccgccgt cgtgaatttg
      241 gcgaaggtga acaattaccg tctgaacgcg aactgatggc gttctttaac gtcgggcgtc
      301 cttctgtgcg cgaagcgctg gcagcgttaa aacgcaaagg tctggtgcaa atcaacaacg
      361 gcgaacgtgc ccgcgtctca cgtccttctg cggacaccat catcggcgag ctttccggaa
      421 tggcgaaaga tttcctttct catcctggtg ggattgccca tttcgaacaa ttacgtctgt
      481 tctttgaatc cagcctggtg cgctatgcgg ctgaacatgc caccgatgag caaatcgatt
      541 tgctggcaaa agcactggaa attaacagtc agtcgctgga taacaacgcg gcattcattc
      601 gttcagacgt tgatttccac cgcgtgctgg cggagatccc cggtaaccca atcttcatgg
      661 cgatccacgt tgccctgctc gactggctta ttgccgcacg cccaacggtt accgatcagg
      721 cactgcacga acataacaac gttagttatc aacagcatat tgcgatcgtt gatgcgatcc
      781 gccgtcatga tcctgacgaa gccgatcgtg cgttgcaatc gcatctcaac agcgtctctg
      841 ctacctggca cgctttcggt cagaccacca acaaaaagaa ataatgccac tttagtgaag
      901 cagatcgcat tataagcttt ctgtatgggg tgttgcttaa ttgatctggt ataacaggta
      961 taaaggtata tcgtttatca gacaagcatc acttcagagg tatttatggc aacgaattta
     1021 cgtggcgtaa tggctgcact cctgactcct tttgatcaac aacaagcact ggataaagcg
     1081 agtctgcgtc gcctggttca gttcaatatt cagcagggca tcgacggttt atacgtgggt
     1141 ggttcgaccg gcgaggcctt tgtacaaagc ctttccgagc gtgaacaggt actggaaatc
     1201 gtcgccgaag aggcgaaagg taagattaaa ctcatcgccc acgtcggttg cgtcagcacc
     1261 gccgaaagcc aacaacttgc ggcatcggct aaacgttatg gcttcgatgc cgtctccgcc
     1321 gtcacgccgt tctactatcc tttcagcttt gaagaacact gcgatcacta tcgggcaatt
     1381 attgattcag cggatggttt gccgatggtg gtgtacaaca ttccagccct gagtggggta
     1441 aaactgaccc tggatcagat caacaccctg gttacgttgc ctggcgtcgg tgcgctgaaa
     1501 cagacttctg gcgatctcta tcagatggag cagatccgtc gtgaacatcc tgatcttgtg
     1561 ctctataacg gttacgacga aatcttcgcc tctggtctgc tggcgggcgc tgatggtggt
     1621 atcggtagta cctacaacat catgggctgg cgctatcagg ggatcgttaa ggcgctgaaa
     1681 gaaggcgata tccagaccgc gcagaaactg caaactgaat gcaataaagt cattgattta
     1741 ctgatcaaaa cgggcgtatt ccgcggcctg aaaactgtcc tccattatat ggatgtcgtt
     1801 tctgtgccgc tgtgccgcaa accgtttgga ccggtagatg aaaaatatct gccagaactg
     1861 aaggcgctgg cccagcagtt gatgcaagag cgcgggtgag ttgttttccc ctcgctcgcc
     1921 cctaccgggt gaggggaaat aaacgcatct gtaccctaca attttcatac caaagcgtgt
     1981 gggcatcgcc caccgcggga gactcacaat gagtactaca acccagaata tcccgtggta
     2041 tcgccatctc aatcgtgcac aatggcgggc attttccgct gcctggttgg gatatctgct
     2101 tgacggtttt gatttcgttt taatcgccct ggtactcacc gaagtacaag gtgaattcgg
     2161 gctgacgacg gtgcaggcgg caagtctgat ctccgcagcc tttatctctc gctggttcgg
     2221 cggcctgatg ctcggcgcta tgggtgaccg ctacgggcgt cgtctggcaa tggtcaccag
     2281 catcgttctc ttctcggccg ggacgctggc ctgcggcttt gcgccaggct acatcaccat
     2341 gtttatcgcg cgtctggtca tcggcatggg gatggcgggt gaatacggtt ccagcgccac
     2401 ctatgtcatt gaaagctggc caaaacatct gcgtaacaaa gccagtggtt ttctgatttc
     2461 aggcttctct gtgggggccg tcgttgccgc tcaggtctat agcctggtgg ttccggtctg
     2521 gggctggcgt gcgctgttct ttatcggcat tttgcctatc atctttgctc tctggctgcg
     2581 taaaaacatc ccggaagcgg aagactggaa agagaaacat gcaggtaaag caccagtacg
     2641 cacgatggta gatattctct accgtggtga acatcgcatt gccaatatcg taatgacact
     2701 ggcggcggct actgcgctgt ggttttgctt cgccggtaac ctgcaaaatg ccgcgatcgt
     2761 cgctgttctt gggctgttat gcgccgcaat ctttatcagc tttatggtgc aaagtacggg
     2821 taaacgctgg ccaacgggcg taatgctgat ggtggtcgtg ttgtttgctt tcctctactc
     2881 atggccgatt caggcgctgc tgccaacgta tctgaaaacc gatctggctt ataacccgca
     2941 tactgtcgcc aatgtgctgt tctttagcgg ctttggggct gcggtggggt gctgtgtcgg
     3001 cggcttcctc ggtgactggc tgggaacccg caaagcgtac gtttgtagcc tgttggcctc
     3061 gcagctgttg attattccgg tatttgcgat tggcggcgca aacgtctggg tgctcggtct
     3121 gttactgttc ttccagcaaa tgcttggaca agggatcgcc gggatcttac cgaaactgat
     3181 tggcggttat ttcgataccg accagcgtgc agcgggcctg ggctttacct acaacgttgg
     3241 cgcattgggc ggcgcactgg ccccaatcat cggcgcgttg atcgctcaac gtctggatct
     3301 gggtactgcg ctggcatcgc tatcgttcag tctgacattt gtggtgatcc tgctgattgg
     3361 tctggatatg ccttcccgcg ttcagcgttg gctgcgcccg gaagcattgc gtactcatga
     3421 cgctatcgac ggcaaaccat ttagcggtgc cgtgccattt ggcagcgcca aaaacgattt
     3481 agtcaaaacc aaaagttaat cctgttgccc ggtctatgta ccgggccttt cgctaaggga
     3541 agatgtatgt cgttacttgc acaactggat caaaaaatcg ctgctaacgg tggcctgatt
     3601 gtctcctgcc agccggttcc ggacagcccg ctcgataaac ccgaaatcgt cgccgccatg
     3661 gcattagcgg cagaacaggc gggcgcggtt gccattcgca ttgaaggtgt ggcaaatctg
     3721 caagccacgc gtgcggtggt gagcgtgccg attattggaa ttgtgaaacg cgatctggag
     3781 gattctccgg tacgcatcac ggcctatatt gaagatgttg atgcgctggc gcaggcgggc
     3841 gcggacatta tcgccattga cggcaccgac cgcccgcgtc cggtgcctgt tgaaacgctg
     3901 ctggcacgta ttcaccatca cggtttactg gcgatgaccg actgctcaac gccggaagac
     3961 ggcctggcat gccaaaagct gggagccgaa attattggca ctacgctttc tggctatacc
     4021 acgcctgaaa cgccagaaga gccggatctg gcgctggtga aaacgttgag cgacgccgga
     4081 tgtagggtga ttgccgaagg gcgttacaac acgcctgctc aggcggcaga tgcgatgcgc
     4141 cacggcgcgt gggcggtgac ggtcggttct gccattacgc gtcttgaaca catttgtcag
     4201 tggtacaaca cagcgatgaa aaaggcggtg ctatgaccac gctggcgatt gatatcggcg
     4261 gtactaaact tgccgccgcg ctgattggcg ctgacgggca gatccgcgat cgtcgtgaac
     4321 ttcctacgcc agccagccag acaccagaag ccttgcgtga tgccttatcc gcattagtct
     4381 ctccgttgca agctcatgcg cagcgggttg ccatcgcttc aaccggaatt attcgcgacg
     4441 gcagtttgct ggcgctgaat ccgcataatc tcggtggatt gctacacttt ccgttagtca
     4501 aaacgctgga acaacttacc aatttgccga ccattgccat taacgacgcg caggccgcag
     4561 catgggcaga gtatcaggcg ctggaaggcg atataaccga tatggtcttt atcaccgttt
     4621 ccactggcgt cggcggcggt gtagtgagcg gcggcaaact gcttaccggc cctggcggtc
     4681 tggcggggca catcgggcat acgcttgccg atccacacgg cccggtctgt ggctgtggac
     4741 gcacaggttg cgtggaggcg attgcttctg gtcgcggcat tgcggcggca gcgcagggtg
     4801 aactggcggg cgcggatgcg agaactattt tcacacgcgc cgggcagggc gacgagcagg
     4861 cgcagcagct gattcaccgc tccgcacgta cgcttgcaag gctgatcgct gatattaaag
     4921 ccacaactga ttgccagtgc gtggtggtcg gtggcagcgt tgggctggca gaagggtatc
     4981 tggcgctggt ggaaacgtat ctggcgcagg agccagcggc atttcatgtt gatttactgg
     5041 cggcgcacta ccgccacgat gcaggtttac ttggggctgc gctgttggcc cagggagaaa
     5101 aattatgatg atgggtgaag tacagtcatt accgtctgct gggttacatc ctgcgttaca
     5161 ggacgcgtta acgctggcat tagctgccag accgcaagaa aaagcgccgg gtcgttacga
     5221 attacagggc gacaatatct ttatgaatgt catgacgttt aacactcaat cgcccgtcga
     5281 gaaaaaagcg gaattgcacg agcaatacat tgatatccaa ctgttattaa acggtgagga
     5341 acggattctg tttggcatgg caggcactgc gcgccagtgt gaagagttcc accatgagga
     5401 tgattatcag ctttgcagca ccattgataa cgagcaagcc atcatcttaa aaccgggaat
     5461 gttcgccgtg tttatgccag gtgaaccgca taaaccagga tgcgttgtcg gcgagcctgg
     5521 agagattaaa aaggttgtgg tgaaggttaa ggctgattta atggcttaaa acttagttcg
     5581 ttttaaagat tctgcagaca ctgtctgcag aattcgaaac cgctgaaatt aacggcgtct
     5641 atagccttcc tctagcaccg cctgtgttcg aacgagttca tctgcgagtc gtttctcatc
     5701 aggtagttgc atcttatatt cgcttgccag aacggtgtta ggcaaacctg ccagagcata
     5761 atgtgcttct ccggctcctt tctctgcaca gagaaccaga ccgatgggcg gattttcatc
     5821 cggtagcgtc cagtgctctt tggcgtagtt gagatacata ttcatctgtc cggcatcgct
     5881 atagctgaat ttgcccactt ttagatcgac gattagtagg cagcgtaaac ggcggtggaa
     5941 aaacagcaga tcgacacgaa accagttgtc atcaatgcgt aaccttcgct gccgaccaac
     6001 aaaggcaaaa tcatccccaa gttccagcat gaaatccatc aggtggttga tcagcgcctc
     6061 ctcgaaatca gattctgaat attcatcttt caattccaga aattcgagca caaagggatc
     6121 gcgtatcgcc tgttgcggaa gaatatgcgt ctcggtagga gcgtgttgtt gcagcatggc
     6181 tgatttgtca tgtgacagta gtgttcgctc ataaaattgg gtcgcaattt gccgttctag
     6241 ctggcgaaca gaccagccac agcggagcgt ctctttttca taaaagctgc gggcgtcagc
     6301 gtttttaacg gaaagtaaac ggacgtaggt ggaccagggc agggggaaag atttggcaga
     6361 cagcgtctgc caaatctttg ccgatgggaa ttctgcagat ggtgtctgcg gaattcccaa
     6421 tggtgttaat tcgccagaca ctgtctggcg aatttcaaca tgttgaaaaa agaggtaaaa
     6481 aagcctcatt tggcgtaagt tttttgcaga gaacccacgc ttatacctta gacttaaatc
     6541 ctttgatagt cgcttgatta gctgcgcacc atacgcagcc ctggcctcgc caccttgttc
     6601 aaattcgaca attcgtcggc caatttccca gtatgtcgcg gtcatcagcg cgttaacgct
     6661 acgtaccgtt tccgtccggg cgctatcgac cagatgaata ataccgtcgt ggatttgctg
     6721 gtagcctgtt gaggttctct ctgagagcga ttccattatc tgtcccttta tatggcgctg
     6781 cgttgaataa tggaatcagc tatacgccgg gcatctaccg gtaacaatcc agatgagcgt
     6841 attacattgg ttttcttcag attatgcgag gcgctttcca gaaagaaagg agaatttcag
     6901 cgccgactct tttctgagga ggcggcgctg ggacagagct taaacttcca gccagttcat
     6961 aataccgtct gccgccttac gaccttcggc aatagcggtt accaccagat cggaaccacg
     7021 gacgatatcg ccgccagcaa agattttcgg gttgctggtc tggaaggcgt tgtcgctgcc
     7081 ttccggggcg atgatgcggc cttgtgaatc cagctcgacg ctgtgttttg ccagccattc
     7141 catgttgtgt ggacggaaac caaacgccat gatcactgca tctgccggaa cgatatgttc
     7201 ggaacctgca acgatctccg cgcggcgacg gcctttggca tccggttcgc ccatttcggt
     7261 acgtaccatt tttacgccgc tgactttacc gttaccgttc acttcaatcc ccagtggctg
     7321 gacgttgaat ttgaactcta cgccttcttc ccgcgcgttt ttcacttcgc ggcgggaacc
     7381 cggcatgttc tcttcatcac gacgataggc acaggtaacg tgcttcgctc cctggcgcac
     7441 ggacgtacgc acgcagtcca tcgcagtgtc gccaccgcca aggaccacca cgcgtttgcc
     7501 ttccatgctg acgaacggtt cgtcgcgggt ttcaccaaag cccattaact gtttggtgtt
     7561 ggcgataagg aacggcagcg ctgcgtacac gccatcggcg tcttcgtttt ccagcccgcc
     7621 gcgcattgac tgataagtcc cgacgccaag gaagacggca tcgtaatcac tcagcagatc
     7681 gtccagctgc acgtcgcggc ccacttcggt attgagtttg aattcaatac ccatgccggt
     7741 gaagatttca cggcgacgcg tcattacctc tttttccagc ttgaaggccg gaataccgaa
     7801 ggtcagcagc ccgccaattt ctggatgacg gtcgaagaca acggctttta cgccgttacg
     7861 cgtcaggaca tccgcacacg ccagacctgc cgggcctgcg ccgataatcg ccactttttt
     7921 accggtttgc ttcacgccgg acatatccgg acgccagccc atttcgaacg ctttatcatt
     7981 gatataacgc tcaatgttgc cgatggtcac cgcgccaaac tcatcgttca gagtgcagga
     8041 accttcgcac agacggtctt gcgggcagac tcgtccgcaa acttccggca gggtgttggt
     8101 ctggtgcgac agttccgccg cttcaaaaat acgcccctcg ttggcgagct tcagccagtt
     8161 cgggatgtag ttgtgtaccg gacatttcca ctcgcagtat gggttgccgc acgacaggca
     8221 gcggtcagcc tgcgctttgg cctggccttc ggaaaacggc tcgtaaattt caacaaactc
     8281 aattttgcgg atcttcagcg gcttctttgg cggatcaacg cgctgcaggt cgataaattg
     8341 ataaacattc tgactcattg ttgctacccc ttactgcgcc tgcacgcgca actctgctgc
     8401 gctacgacta cgatgaccca gcagtgcttt tacatcactg gacttcggtt taaccagcgc
     8461 aaatttagtg gcgaaggttg accagttcgc cagaatctct tcaccgcgct gagagccagt
     8521 atgctgaaca tgctcggtga taagaccgcg cagatgctct tcatggatcg ccagatcgtc
     8581 aacgcttaag acctcgacca gttccgggtt aacgcgtttg cggaaatcac cgctttcatc
     8641 gagaacgtaa gcgaagccgc cggtcatgcc cgcaccgaag ttaacgccgg ttttacccag
     8701 aatacagaca ataccgcccg tcatatattc acagccgttg tcgccaatgc cttctaccac
     8761 ggtgatagca ccggagttcc gcacgccgaa acgttcaccc gcgcggcctg cggcatacag
     8821 acgaccaccg gtcgcgccat acaggcaggt gttgccgata atacttgctt catggctgcg
     8881 gaaggcggaa ccaaccggag gacgaatggc gattaagccg cccgccatgc ctttaccgac
     8941 atagtcgttg gcatccccgg tcaggtacag ttccacgccg cccgcgttcc acacaccgaa
     9001 actctggcct gcggtgccgt tgaagtacgc tttgatagga tcggctgcca gcccctgatc
     9061 gccgtgcgtc tgggcgatat agcctgaaag cgacgcgccg acagaacggt cggtgttgcg
     9121 aatatcgaac cagaaggttt tgctctggcg ttcatcgaca aacggtttcg cctgttgcag
     9181 caactgcgcg ttcagcaggc cgttatcaaa cggtgggttg ttttcggtgc agtagagtgc
     9241 cttacctgga tgcggttcgg cggtctccag cagcttcgac agcgccagtt tttgctgttt
     9301 ggcggtgaaa ccgtccagtt ctttcagcag gtcggtgcga ccaatcagat ccaccagacg
     9361 cgttacgccc agctgtgcca tcagctcgcg ggtttcacgg gcaataaatt caaagtaatt
     9421 ggtaaccttg aacggcaggc cgtgatagtg gttcttacgc agtttgtcat cctgagttgc
     9481 tacacccgtt gcgcagttgt tcagatggca aatacgtaga tatttacagc cgagcgccac
     9541 catcgggcca gtgccgaagc cgaagctttc tgcgccgaga atcgccgctt tgatgatatc
     9601 aacaccggtt ttcaggccgc catcgacctg caaacggatc ttatgacgca gaccattggc
     9661 aaccagcgcc tgctgggttt caacaagccc cagctcccac gggcaacctg cgtatttcac
     9721 tgacgacagc gggcttgcgc cggtgccacc gtcatagcct gcgatggtga ttaaatcggc
     9781 ataagctttt gccacgccag tcgcgatggt gcctacgccc ggttcggaaa ccagcttcac
     9841 ggagatcatc gctttcgggt taacctgctt gaggtcgaaa atgagctgcg ctaagtcctc
     9901 gatagagtag atatcgtggt gcggcggcgg ggagatcagc gtcactccgg gcaccgaata
     9961 gcgcagtttg gcgatgtaag gagtgacttt gtcgcccggc aactgaccgc cttcgcccgg
    10021 cttcgcgccc tgggcgactt taatctgaat gacgtcggca ttgaccagat acgccggagt
    10081 aacgccgaag cgaccagaag ccacctgctt gatgcgcgac actttgttag tgccatagcg
    10141 cgccgggtct tcgccgcctt caccggagtt cgagttaccg ccgatgctat tcatcgcttc
    10201 cgccagcgcc tcgtgggctt ccgggcttaa cgcgccgata gacatcgcgg cggtatcaaa
    10261 gcgtttaaac agttcgcttg ccggttcaac atcagcaatg ttgaccgcgt tttcacccgg
    10321 cgtaattgcc agcagatcgc gcagcgtggt tgccggacgc tcattaacca gcttcgcgta
    10381 ttcctgatag tcgctgtact cgccgctttg taccgcctgt tgcagcgtgc gcaccacgtc
    10441 cgggttgtaa gcgtggtatt cgccgccgtg gacgtatttc agcaggccgc cctggctgat
    10501 gggcttacgc gccagccagg cacgtttcga caggttcagc agatcctgct ggaagtcttc
    10561 aaagctcgcc ccaccaatgc ggctgaccgc cccctggaag cacaggccca ctacatcatc
    10621 gtgcagaccg accgcttcaa acagtttcga gcagcggtaa gaggcgatgg tggagatgcc
    10681 cattttggac atgattttgt acaagccttt gttgatgccg ttacggtagt tgagcatcac
    10741 ggtacgataa tctttggcaa tcgcatgggt gtctaccagg cggcccagcg tttcataggc
    10801 aaggtatgga taaatagccg tcgcgccgaa gcccagcaac acggcgaagt ggtgcggatc
    10861 gcgggcgctg gcggtttcga cgatgatgtt ggcatcgcaa cgcaggcttt gatcgaccag
    10921 acgggtctgg atcgcgccaa ccgccatcgg ggctggaacc ggcaggcgat ctttagcgat
    10981 attccggtcg gagagcacca gcagcacggt gccgctacgt accatttttt cggctttgtc
    11041 gcacagctct ttgactgtcg cttcgagcgt ggttttagtg acgtcaaagg tgatatccag
    11101 cgtatctgcg cggtagtgct cctctttcat cgtcgtgagc tgtttgaaat cggagtagag
    11161 cagaatcggc gatttaaagc ttaaacggtg cgcctggccc tctgcttcgc aaaagacgtt
    11221 catttcacga ccgatactgg tggcgagcga cataacatgc gcttcacgca gcgggtcgat
    11281 tggcgggtta gtcacctggg caaactgctg gcggaagtag tcgtaaataa tgcgcggctg
    11341 actggagagc acggcgaatg gggtatcatc gcccatcgaa ccgagcgctt cctgaccgtt
    11401 ttcgcccagt acgcgaatta cggagtccag ctcttccgcg ctgtagttaa actgtttctg
    11461 gtagctggca agcgtgtcgt cgtccagttc gcggctaccc acttcttcat cgggcagatc
    11521 ttcaaacggt accagtcggc ggacgttttt ctccatccac tctttatatg gatggcggct
    11581 tttcagatcg tcatcggttt ctgccgagtg cagaatacgc ccactgcggg tgtcgatcac
    11641 catcagttcg cctggcccga cgcggccttt ttcgaccact tcgtcaggct gataatccca
    11701 gataccgact tcagaggcgc aggtgatgag cttgtctttg gtaatgacgt agcgtgccgg
    11761 acgcagaccg ttacggtcga ggttacaggc agcgaagcga ccatcggaca tcacgatgcc
    11821 cgccgggcca tcccacggct ccatatgcat ggagttaaag tcaaagaagg cacgcagttc
    11881 cgggtccata tccgggttgt tctgccaggc gggtggtact aatagacgca tggcgcggat
    11941 gatatccatc ccgcctgcca gcagcagttc cagcatgtta tccatcgaac tggagtcaga
    12001 accggtttcg ttgacgaacg gtgcggcgtc gtgcagatca gggatcagcg gcgtctggaa
    12061 tttataggta cgcgcacgcg cccattggcg gttaccggtg atggtgttga tttcaccgtt
    12121 atgcgccagg tagcggaacg gctgcgccag cggccagcgc ggtacggtgt tagtggagaa
    12181 gcgctggtgg aacaggcaga tggccgattc cagacgcagg tccgcaagat ccagataaaa
    12241 gcgcggcaga tccgccggca tacacagacc tttatagatg ttcaccagat tcgacaggct
    12301 acagacgtag aagtctttgt cggcttcgag acgcttttca atgcggcggc gggcgataaa
    12361 cagacggcgc tccatatcgc gcggacgcca gcctgccgga gcgttaacga aaatttgctc
    12421 aatgtggggc agagaggaga gggcgatttc acccagcacg ccttcgttag tggggacatc
    12481 acgccagccc acaatcgaca aggtttcgcg ttgcagctct tcttcaacga tgcggcgtgc
    12541 ggcagcggcg agttcaggat ctttattcag gaagagcatc ccgacagcgt agttttttgc
    12601 taaacgccag ccgcgctcct gcgcaacgat gcgaaaaaag cgatccggtt tttgtaacag
    12661 caagccgcaa ccgtcgccgg ttttaccatc ggcgagaatc gcgccacggt gctgcatgcg
    12721 ggccagtgcg tgtattgcag tacgcactac cttgtggcta ggttcgcctt ctatgtgggc
    12781 gatcaggccg aaaccacagt tatccctctc aagggattta tcgtacaaca tatcgtgaac
    12841 ctcccccagg ctctgcggga accccacttc cgaaccgctg cgcacgggca cagaaagagc
    12901 gtggcgacgg ggtttgcgtg tcatacgcgc gcctcgcatt cgcccccttg ttgtcctttt
    12961 cgcatcggtt aatacggtga agaggggatc tcaattactg cataaatatg atgagcaggc
    13021 tgctcatcca gaaagcttcc agcggatttc caacttatcg ggaaagctga tacaggtcaa
    13081 atggcaagct tattggtaca gaaatgtgct ttagcgcaaa attctttatt aaattactga
    13141 aattaaaatg gattttatct ttaaggctaa aagggaagtc gttagagaaa cagtcaattc
    13201 ctgactgtca taaaggcgtg ttaaccctta tcctgccttg aactgacgag aaaaccagca
    13261 ttttatactg ccttaattgg taatgacgtt tcatgctact gtttttgcct aaaatccata
    13321 aggagataaa aaaatctgac acaagataaa caaaagtgat ttatgtgatt atgcatgaaa
    13381 tttcggtaat tttgattgat tatgcagttg ataaaagcag gccgggacga ttgatccagg
    13441 tcatcgccga attgagctaa aaatggcagg ctttggcctc ttttcaaggc gcggtctatc
    13501 agattatgca gttacagaaa ttagtcaata tgtttggtgg tgatctcacc cgtcgttatg
    13561 ggcaaaaggt gcataagctg acgctacacg gcggttttag ctgccctaac cgtgacggta
    13621 ccatcgggcg tggtggctgc acattctgta atgttgcctc gtttgccgat gaagcgcagc
    13681 agcatcgttc cattgccgag caactggcgc accaggcgaa tttagttaac cgcgctaaac
    13741 gctatctggc ctactttcag gcgtatacca gcacctttgc ggaagttcag gtgctgcgtt
    13801 ctatgtatca gcaggcggtg agccaggcca atattgtcgg tttgtgtgtt ggtacccgcc
    13861 cggactgcgt gccggatgcg gtgctggatc tgctttgcga atataaggac cagggttacg
    13921 aagtgtggct ggagctgggg ctacaaaccg cccacgacaa aacactgcat cgcatcaacc
    13981 gcggccatga ttttgcccgt tatcagcgta caacccagct ggcacgtcag cgcgggctga
    14041 aggtgtgttc ccacttaatt gtcggcctgc ctggcgaagg gcaggctgaa tgtttgcaaa
    14101 cgctggagcg agtggttgaa accggcgtgg acggcataaa gctgcatccg ctgcatattg
    14161 tgaaaggcag cattatggcg aaagcctggg aagcggggcg tttgaacggt attgaactgg
    14221 aggattacac gctcactgcc ggagaaatga ttcgccatac gccaccggaa gtgatctacc
    14281 accgcatttc cgccagcgcc cgtcgcccga cgctgcttgc tcctctgtgg tgcgaaaatc
    14341 gatggacggg aatggtcgag ctggatcgct atctgaatga acatggcgta cagggatcgg
    14401 cgctggaacg tccgtggata cctccaacgg agtaggtcgt tgaggggaat tccgcatttc
    14461 tcacacaatt tataacgtaa ctgtcagaat tgggtattat tggggcaggt tgtcgtgaag
    14521 gaattcccta atgaagcaaa ttcgtctgct ggcgcagtat tatgttgacc tgatgatgaa
    14581 gttaggtctg gtgcgcttct caatgttgct ggcgctggcc ctcgtcgttc ttgccattgt
    14641 ggtacaaatg gcggtaacca tggtgctgca tggtcaggtc gaaagcattg atgttattcg
    14701 ttctatcttc tttggtttgc tgattacgcc gtgggcggtc tactttctat cggtggtcgt
    14761 cgagcaactg gaggagtcac gacaacgtct gtcacggctg gtgcaaaaac tggaggagat
    14821 gcgcgagcgc gatttgagcc tcaacgttca gttaaaagat aatattgccc agctaaatca
    14881 ggaaatcgcc gttcgtgaaa aagcggaagc agaactgcag gaaaccttcg gccaactgaa
    14941 aattgaaatc aaagagcgcg aagagacaca aattcagctc gagcagcaat cctcattctt
    15001 acgttccttc cttgatgctt cacccgacct ggttttttat cgtaacgaag ataaagagtt
    15061 ttccggctgt aaccgcgcga tggagctgct gaccggaaaa agcgaaaaac aactggttca
    15121 cctgaaacct gctgatgttt actcaccgga agccgccgca aaagtcattg aaaccgatga
    15181 aaaagtgttc cgtcataatg tgtcactgac ctatgaacag tggctggatt acccggacgg
    15241 gcgcaaagcc tgctttgaaa tccgtaaagt gccgtactac gaccgcgtgg gtaaacgcca
    15301 cggtttgatg ggctttggtc gcgacattac cgagcgtaag cggtatcagg atgcgcttga
    15361 acgggccagc cgcgacaaaa cgacgtttat ctccaccatc agtcacgaat tgcgtacacc
    15421 gctgaacggt atcgtcggcc tgagccgtat tctgctggat accgaactca ccgccgagca
    15481 ggaaaaatat ctcaagacca tccatgtttc ggccgtcacg ctggggaata tctttaacga
    15541 tattatcgac atggataaga tggaacggcg caaggtccag cttgataatc aaccggttga
    15601 tttcaccagc ttccttgccg atctggaaaa tctctccgca ttgcaggcgc aacaaaaagg
    15661 attgcgcttt aacctggagc cgacgctgcc attaccgcat caggtcatta ccgacgggac
    15721 gcgtttacgg cagatcctgt ggaacctcat cagtaacgcc gtcaaattca cccagcaagg
    15781 cctggttacc gtgcgcgtgc gctacgatga aggcgatatg ctgcattttg aagtggaaga
    15841 ctctggtatc ggcattccgc aggatgagct ggataaaatt ttcgccatgt attaccaggt
    15901 gaaagacagt catggcggta aacctgccac cggcaccggt attggtctgg ccgtttctcg
    15961 tcgtctggcg aaaaatatgg gcggcgatat tacggttacc agcgaacagg gcaaaggttc
    16021 aacctttacg ttgacgatcc acgcaccgtc ggtagcagaa gaggtcgatg atgcgtttga
    16081 tgaagacgat atgcctttac cggcgctgaa tgtgctgctg gtggaagaca ttgaactgaa
    16141 cgtgattgtt gcgcgttctg tgctggaaaa attaggtaac agcgttgatg tcgccatgac
    16201 cggcaaggcg gcgctggaga tgtttaaacc gggcgaatac gacctggtgt tgctggatat
    16261 tcagttgcca gatatgaccg ggctggatat ctctcgtgaa ctgacgaagc gttatccgcg
    16321 cgaggattta ccgccgctgg tggccttaac cgctaacgtg ctgaaagaca aacaagagta
    16381 cctcaatgct ggaatggatg atgtgctgag taagccgctt tcggttccgg cgctaaccgc
    16441 gatgatcaag aaattctggg atacccagga tgatgaggag agtacggtga cgacagaaga
    16501 gaacagtaaa tcagaagcat tgctcgatat tcccatgctg gaacagtatc tcgaacttgt
    16561 aggaccgaag ctgatcaccg acgggttagc ggtgtttgag aagatgatgc cgggctatgt
    16621 cagcgtgctg gagtcgaatc tgacggcgca ggataaaaaa ggcattgtag aggaaggaca
    16681 taaaattaaa ggtgcggcgg ggtcagtggg gttacgccat ctgcaacagc tgggtcagca
    16741 aattcagtct ccggaccttc ctgcctggga agataacgtc ggtgaatgga ttgaagagat
    16801 gaaagaagag tggcgtcacg acgtagaagt gctgaaagcg tgggtggcaa aagccactaa
    16861 aaaatgaccc cggctagacc ggggtgcgcg aatactgcgc caacaccagg gaaatcttgg
    16921 ctgcgccgta aattattatg atgagttaca agggcacagc actgtttttc aggccgcacg
    16981 cagttaagat agcaaaactt aaatgatttg ttacatgaat cagttaaatg tgtgatgcgt
    17041 accattttta ttaaaaaaat taatgtgttt cagtcaattg cgaaaggatg atcacaatga
    17101 agaaaattgg cgtaattctg agcggatgcg gcgtctatga cggttctgaa attcatgaag
    17161 cggtgttgac gttgttagct atttcacgca gcggtgcgca ggcggtctgc tttgcaccgg
    17221 ataagcagca ggttgatgtt atcaaccatt taactggcga agcgatgacg gaaacgcgca
    17281 acgtgctgat tgaagcggca cgaataacgc gcggtgaaat ccgtcctctg gcccaggccg
    17341 atgccgctga actggatgcg ttgattgtgc cgggggggtt tggcgcggcg aagaatttaa
    17401 gcaattttgc cactcttggt agcgaatgca ccgttgaccg tgaattaaag gcgctggcac
    17461 aagcgatgca tcaggccgga aaaccgcttg gttttatgtg tattgccccg gcgatgctgc
    17521 cgaaaatttt cgatttcccg ctgcgtttga ccatcggtac tgatatcgat accgcagaag
    17581 tgctggaaga gatgggcgcg gagcatgtgc cgtgtcctgt cgatgatatc gtggttgatg
    17641 aagataataa gattgtcacc accccagcat atatgctggc gcagaacatt gcagaagcgg
    17701 cgagcggcat tgataagctg gtttcccgcg tgctggttct ggctgaatga gtaaaagccg
    17761 cttaacggtg tttagtttcg ttcgccgttt tcttttgcgg ttaatggttg tcctcgccgt
    17821 tttctggggc gggggcatcg cgttgtttag cgttgcgcct gttcccttct cagcggtaat
    17881 ggtcgagcga caggtcagcg cctggctgca tggcaatttt cgttacgtgg cacattctga
    17941 ctgggtcagt atggatcaaa tctcgccatg gatgggactg gcggtgattg ccgcagaaga
    18001 tcagaaattt cctgagcact ggggctttga tgtcgcttcc attgagaaag ccctggcgca
    18061 caacgagcgc aatgaaaacc gtattcgcgg tgcatcaacg atttctcagc agacggccaa
    18121 aaatctcttt ttatgggatg ggcgtagctg ggttcgaaaa gggctggaag ccggattaac
    18181 gctggggata gaaacggtct ggagcaaaaa gcgtatcctg acggtttacc tgaatatcgc
    18241 cgaatttggc gacggtgtgt ttggcgtcga agctgcggca caacgttatt tccacaaacc
    18301 cgcgagcaaa cttacccgat cggaagctgc attactggca gctgtattac ctaatccact
    18361 tcgtttcaaa gtctccgcgc catcgggcta cgtgcgtagc cgtcaggcgt ggattttacg
    18421 gcagatgtat cagttaggcg gtgagccgtt tatgcaacaa caccagctgg attaagcttc
    18481 gcccgaagtg agggccagcg ttttgctctc acgaggttgc agatctttct gcagggcaat
    18541 agccagcgcc ggttgggcaa taaatcgttt ccataaccgc tcttgcttac gcagacaaca
    18601 ccacttcgac caggtcgcca gtggaatcaa tgtgctttca ccaatgttgt cgcagacgac
    18661 ggggataacc tctgactcac taatgcggtg acagaggata ttctgcggct tcaacgacat
    18721 cgtcacgata cggttatcct gcaaataacg cttcagctgt ttcagtaact ggcgcagttg
    18781 tgcaatgtct tcttcgtaac ggcattgttc agcaaattcg gtcagcgtaa tggaaggctt
    18841 gccgtcaaaa tcggcaatca catcgtagac ataaccggtt ccgcaatccg tctcaacggt
    18901 accgtgatag cgcggtattc cactccagtc ttttaaccgg cgaccaagat gcgcgtaata
    18961 ctttaactca cggcggatct ctttatcgcc accatcgcca cgatggtaga caatcttgat
    19021 acagcgttgg gcatcttccg gatgcgcata acatttccga tgtcttccgg tgcccagggg
    19081 actttgttca gataagcgaa tcatgccatc tcctggaaat attttctgac gatctccagt
    19141 atggtcgatt aacttaaacg aaacctaaac aatgcttctt aaggttttct taaggttctt
    19201 ctgaaagtaa aaagcgcacg aaatgtacaa ttgacgttga ttgagaacga tttcagtacg
    19261 cgcttttctt attgccgggg gagcttgaag ggatttgaat gtcaaagtga tgaagattaa
    19321 tcttcatcaa aaccagagtt aaagagggcg ataacggcgg ccagtgcttc ctcttcctgt
    19381 ggaccggtcg cttcaacttc aatctgccgt cctttggcag aatccaacat cagcagcgca
    19441 ataacgctgt tggcttcagc ctcggtgcct tcgtcattac gtaacagcac ttcagcgtca
    19501 aaaccctgca ttaattcaaa cagcttcatt gcaggccggg catgcatgcc cagcttgttt
    19561 gtgatttcaa cagtttgctt gacggtcatg gtttacgttt ttccagcgta cgatggcgtg
    19621 actggacgtt tttaccgcgc gagcggaagt agtctgccag ttgctctgca atatacaccg
    19681 aacggtgctt cccgccggta caaccaatgg cgaccgtcag gtagctacgg ttgttggttt
    19741 ccagcatagg taaccatagc tcaagatagc ttcgcgtctg gtagataaaa ttgtgtactt
    19801 ctgtgtggcg gtcgaggaac gcggcgacag gcttatcaag acctgtcatt ggacgcagtt
    19861 tcggatccca gtgcgggttc ggcaagaagc gcacgtcaaa gacgtaatct gcatcgatag
    19921 ggataccgtg tttgaagccg aaagactcaa agaccattgt cagttcgcgt tcacgtttac
    19981 ccagcagacg ggtacgcagc atttctgcca gctcgtgaac ggacatttct gaggtatcga
    20041 caatcagatc cgctcgcgaa cgcagaggct ccagcagatc actttctttg tcgatagcac
    20101 tttccagcga caggtttttg ctggaaagcg gatgcagtcg gcgcgtgtca ctgtaacgac
    20161 gaattaaggt attacggtcg gcatccagga acagtagttg cggtgagaaa gcgtcaggca
    20221 ggttactcat cgcctgttcg aatatttctg gtgactccgg catattacga acatcaatgc
    20281 tgacggcggc agaaatctcg cgatcggcca gggttcgagc cagatcgggt aacaacacta
    20341 cgggaaggtt atccacgcag taaaaaccca tatcttccag cgcacgcagg gcgacagatt
    20401 tacctgaacc tgaacgtccg ctgacgatca tcagtaccat gtactgtttc tcctcacaac
    20461 gtctaaaaga gacattaccg aataactacg cttcatccgg agtaccttcg gtatccgtaa
    20521 tgatttgata cagctcttca tcgctctggg ctgcgcgtaa acggcggcag atggttttgt
    20581 ccgccagacg tttcgccacc agcgacagag tatgcaggtg cgttttagtt tggtctgccg
    20641 gcaccagcag ggcaaaaagt aagtccaccg gttggttgtc gatggcatcg aaagctatag
    20701 gcgtttcgag ctgaacgaaa acgccaacgg cacgcagagt atcttcttcc agtttgccat
    20761 gcggaatggc aataccattg ccgataccgg tactgcccat tttttcccgc gtcaggatag
    20821 cttcaaaaac cacctgaggc ggcaggctaa gttgtttcgc cgccagctcg ctgatgattt
    20881 ccagggcgcg ttttttgctc tgacagtgga cgcggcttcg cgtacattcc ctgttaagaa
    20941 cactgctaag ctgtagagtt gtatcattat ttgtcataat ttcacctaag aacctgccca
    21001 ctcaaacagt ggaacaggct cggagcatta accgtacaaa tggcccgttg tgccgcacaa
    21061 caggccgcca tgcacatgct aattgcccgg acaattagtg ttgtttcagt ttatctttat
    21121 gtttggtcaa ctggcgtgcc agcttatcaa ttaagccatc aatggcagcg tacatgtcct
    21181 gaccttccgc gctggcatga atttcaccac cgtttacatg cagtgttgca tctgaggtgt
    21241 gggtgacttt ctccactttc agaacaacat agacctggtt gattcggtca aaatattgct
    21301 caagtttggc aaatttggct gtaacaaatt cgcgcagtgc ctcggtgatc tcgacgttat
    21361 ttccggtaat gttgagctgc atagtgtctt ccttatcggt tgggtcaaac gagctgttta
    21421 cgctggtttg acggcggaat ggataaagac tctcggtact tcgcaacagt gcggcgtgcc
    21481 accatgatac cttgttccga cagcaaagag gttaacttgc tgtcgctcaa cggtttcgct
    21541 gggttttccg ccgcgattaa tttcttcacc agcgcacgaa tcgccgtgga ggaagcttcg
    21601 ccgccgccct cggtattgac gtgactggaa aagaaatact tcagttcaaa aatgcctcgt
    21661 ggactatgca ggtatttttg cgtggtcacg cgagatatcg tcgattcatg catttcgaca
    21721 gcctgggcga tatcggccag taccatcggt ttcatatatt cttcaccttg ctcaaagaag
    21781 gcttgctgct gttcaacgat acagcgactc acgcgcagta gcgtatcgtt acggctttcc
    21841 agactcttga tcaaccattt ggcatcctgc agattgctgc ggataaactg gctgtcacca
    21901 tcgttgcgcg cgttattgca catcgaggcg tagtgctggt tgatttgcag acgcggaatg
    21961 ctgtcactgt tgagttctac cgtccagtga ccgttatgct tacgcaccag cacatctgga
    22021 atgacatact caggttcgcc agtctggatc gactgcccgg ggcgcggatc gagcgactgg
    22081 atcagattga cggcttcttt cagcacatct tctttcagac gcgtgacgcg cattaaagtg
    22141 cggaagtcgt gattggctaa cagatcgaga tgatcgctaa tgatcaatct ggcctcttcc
    22201 agccacggcg tggtcttatc gaattgggag agttggatta gcaggcagtc acgcagatct
    22261 tttgccgcta caccgaccgg atcaaaccgt tggatccgct taaggacggc ttcaacctcg
    22321 tcgatgtcga tctcttcatc gcccatactt tcgagaatat cttccagcgg gacagtcaga
    22381 taaccggtgt catcaacggc atcgacgata gaggtagcaa tagcgcggtc agtgtcggaa
    22441 aacggtgtca gctcgacctg ccacatcagg taatcctgca aggtctgcgt cgtttcgccc
    22501 tgatagaccg gcagctcatc gtcaatgtag tcaccgctgg tgccggatgg tgtaccagcg
    22561 gtgtaaatgg tgtcccaact ggcatcgagc ggcagctctt ccggcatctc tttttgttcg
    22621 agcgcgtcgg cggtgtccag cgtttcactg tcttgcgttt cgcgggtgtc gatttcttca
    22681 tgagtgtcga tttgctcaag cagcggatta ctctccagcg cctgctgtag ctcctgctga
    22741 agttccagcg tcgacaactg caacagacga attgcctgtt ggagctgtgg cgtcatcgcc
    22801 agttgttggc taagcctgag ttgcaaacct tgcttcatgt tcagaatcgt actctcctgc
    22861 taaaacgtcg caaacttcta ccctatcaga gtctgaagtc ttccccaagg tatacacgct
    22921 taacgtgttc gtcttgtaag atttctgtag gcgtgccgtg ggcgatcaaa tgcccctgac
    22981 tgacgatata agcgcgttca caaaccgcca gtgtttcacg cacgttgtgg tcagtgatca
    23041 gcacgcccag gccgctgtcg cgcaggtgct caatgatgcg tttaatgtcg ataaccgaga
    23101 tcgggtcaac cccggcaaac ggttcgtcga gcagaataaa tttcggattc gcagccagtg
    23161 cgcgggcaat ttctacacgg cgacgttcac ccccggagag tgactgcccc atgctgtcac
    23221 gcaggtgctc aatgtgaaac tcttccatca gctcgttcgc gcggtcttca cgttgttcag
    23281 cagacaagtc gtcacgaatt tgcagtaccg ccatcaggtt atcgtaaacg ctgaggcgac
    23341 ggaaaatgga ggcttcctgt ggcagatagc cgataccgcg gcgcgcgcgt gcatgcagag
    23401 gcagcagact gatatcgtca tcatcaataa tgatgttgcc cgcatcgcgc ggcacaatgc
    23461 caacaaccat atagaaagtg gtggtcttac cggcaccgtt tggccccagc agaccgacaa
    23521 tttccccgga gttgacggtc aggctgacgt cttctaccac gcgacggcct ttataggctt
    23581 ttgcaaggtt ctttgcagtt aatgttgcca taacgaatta attacccttc ttctgtgccg
    23641 gggtctggcc tttgttgttt ttgtcctgca gctgcgacgg caccagaacg gtggttacgc
    23701 gcttgccttt gtcgctgaaa gcctgcattt tctgctcttt taccaggtaa gtgatcttat
    23761 cgcccttaat gttgctatcg acctgctgca gataagcatt acccgtcaga acgacaaaat
    23821 cttttgccag ttcgtagtgc atctgggaag cgtgaccttc aacgggttta ccgttgtcct
    23881 gcatctggta gaacgttgcc ggtttaccgt agccgtcaat cacttcttta ccttgttcgc
    23941 cgcccggacg ggtaacgacc actttgtcgg cattaatttt gatggtgccc tgggtgacga
    24001 tgacattacc ggtaaaggta accacgttgc cttgcatatc aagagattgc tggtccgatt
    24061 caatgtggat cggctgatca gtgtctccgg ttacggcaaa tgccggaatg ctggcggcca
    24121 gaagtgagct ggcaagcaca agattaaggc tgagtttgtt tgttttgaat ttcataggat
    24181 gttctaacct tttcaatcag ctcggcgttc ttgctgcgta agttgccgcg cattttcaga
    24241 ccgctggagt taaatgttgt tccgtataac gtgacgaggt cttcagaggt aacatcctga
    24301 gtcaccagat tgatctgcgc gttatccgtc gtgattctgc gaagttgaga gtctggcaca
    24361 agtgcgttga cttcaacgtg tccatataaa tagagcatcc ggtcattggt cagcttggct
    24421 ttatctgctt ttacggacca tgtcgggatt ttatccttat caaacgtggt aagtaccggc
    24481 tgcgtaaacc acgaaacggc ctgatcggaa taatattcaa cgtgttgagc aatcaatcga
    24541 tagcttagtg ccccttctgg gttatagacg agcgtgtccg tatgctcgct tttataggtg
    24601 ggatcattgt tgttgacgac cacctgggcg gtatcgtctt tttcggccat attaatgccg
    24661 atcatcacca gaaccgccag tgatagcaca atgataaccc aacgtctggc tttactcata
    24721 tcgattgccc tttggcttca tccagtttgc cctgcgccag gagtaataag tcgcaaactt
    24781 cgcgcactgc gccacgaccg ccagcaatgc gcgtcacgta atcggcgcgc gggatcaaca
    24841 gtggatgcgc atcggccacg gcgacgctta aacccacttt ttccattacc ggccagtcga
    24901 tgagatcatc gccgacataa gccacatttt ccggggcaat cgccaatttt tccagcagat
    24961 cgctaaaggc gatcagttta tttgactgcc cctgatacaa gtgagtgatc cccaatgtgg
    25021 cacaacgatc ttctaccagt ttagcctttc gcccggtaat gatagcgact tcaatatcag
    25081 aggtgagcgc acaacgaatg ccataaccgt cacgaacatt gaacgctttc agctcttcgc
    25141 cattattgcc catataaatc aggccatctg acagtacgcc atcgacatcg aggatcagca
    25201 gacgaatgtt ctctgctttt gctataacgt cggcgctgac agggccgtaa caggtcgcaa
    25261 gcgacgcacc tgctttgctc attgttgttt atccttgaat ctttacacta cgcctgcacg
    25321 cagtaaatca tgcatatgta acacaccgag taaatggtcg ccatcggcaa ccatcacgga
    25381 ggtgatatgg cgggactgca ttaagttcag tgcctcaacg gccagaatgc cagggcgcac
    25441 acgtattccc cccggcgtca tcacatcggc aatacttaac tgacgaacat ccacgcccat
    25501 atcgaagaca cggcgtaaat caccgtcggt aaagatgcct tcaatcatca tattgtcatc
    25561 gcaaatgaca gtcataccaa gatttttgcg ggtaacttcc agcaacgcgt cacgcagact
    25621 ggccgttttc ttaacatgcg ggatctcatc gcccgtatgc ataatatcgt ttacgcgcag
    25681 cagaagttta cgacccagtg cgccgcctgg gtgtgagagc gcaaaatctt cagcagtaaa
    25741 gccgcgtgct tttaacagcg cgacagcgag ggcatcgccc ataaccagcg tggcggtggt
    25801 gctgctggtc ggtgccagcc ctaacggaca ggcttctttc gctactttaa cacacagatg
    25861 cacatctgcg gcgcgcgcca tgctgctctc cgggcgaccg gtgatgcaga ttaacggtac
    25921 gtgaagacgc ttaagcactg gaattaaggc cgtgatttcg ctggattcac ctgagttaga
    25981 gatagcaatc accacatcct gtggggtgac catgcctaaa tcaccatgcg cggcttcacc
    26041 aggatggacg aaaaatgaag gtgtaccggt gctggcaaac gttgcggcca ttttgcgccc
    26101 gatgtgcccc gatttcccca tccccatgac gacaactttc cctttacacc agaacatctt
    26161 ttcacaggca agcgtgaaat tctgattgat gtattgatca agctccgcca ggcattcacg
    26221 ttcaatcgcc aggacttctt tacctgcttg ctgaaagtca aaacccggtt gtaactctac
    26281 gtgcgacata atgcgtttcc agttattcaa cgagtattgg cgataaccag tacaacatcg
    26341 ccagccatac gataaatccg ccagttaata atacccctac accacggccc ggttgtgggg
    26401 agcgccgcca gcacagcaac gcaaaaataa tgctcaccag caacatcacg ctgtagtcac
    26461 gactgtacgc cagtggatca atctctcctg gcgttatcag cgcgggtaaa cccaacacga
    26521 tgacaatatt aaaaatgttt gcgccaatga tatttccgac agcaatgtcg ttttcacctt
    26581 tgcgaacccc cgctattgcg gttgccagtt ccggcaggct ggttccgata gcaattgccg
    26641 tcagacccat cgtcaactcg ctgatggcaa agtaattcgc cagcaccgtg gcgttatcaa
    26701 ccaccatccg cgtggccact ggcatgatga taagcgcaat gccgagccat aaaaacgcga
    26761 cgggcaatcc gccgtcacgc ggcagctctg caagctgctc tctggtcagg ctgtcagtcc
    26821 cctgacgttc agcctgacgt gcaagtttaa caatgaacag cagccatagc acagccagaa
    26881 agaggagaaa gataccatcg ctgcgactaa gttgtccgtc atagagtacg gaaccggcca
    26941 ccacgctgac caacaacatt aagggtaatt cacggcgtag aacatcagaa tggacggtaa
    27001 aaggacgaac cagcgcagcc agaccgagga tcagcaatat attgataatg tttgagccga
    27061 gggctgtacc aacggctaaa tcgcgttgtt cgtgcagaga cgcggcaagc gagacgatga
    27121 tttctggtaa cgatgtacca atactgacca ccgtcatgcc gatgatcagc ggcgggatgc
    27181 caaaggttcg gcaaagaata gacgcggcaa aaaccaggcg gtcggcactg taaacgacca
    27241 aaagtaaacc aacaattaac agtgccgtag ctaaaagcat ctaacgtcct ttcttcaggt
    27301 atactcgccg gtccgctgaa gattt
//
LOCUS       DAZGAN020000058        27118 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGAN020000058 DAZGAN020000000
VERSION     DAZGAN020000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.058"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..353
                     /locus_tag="ACHKYO_RS13625"
                     /old_locus_tag="pgaptmp_002725"
     CDS             <1..353
                     /locus_tag="ACHKYO_RS13625"
                     /old_locus_tag="pgaptmp_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4429547.1"
                     /translation="RGVGTGENQIPDMASFSGVRDYYGKQLLPGGLILQWLTIPSSAA
                     AKAVTLNNGNYQLSGYKWPQSFGVLFAVFATKVSGSTNEAYAISVNRHSTDVIVTWNA
                     RKADDVHILGIGKL"
     gene            350..769
                     /locus_tag="ACHKYO_RS13630"
                     /old_locus_tag="pgaptmp_002726"
     CDS             350..769
                     /locus_tag="ACHKYO_RS13630"
                     /old_locus_tag="pgaptmp_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HGU4429548.1"
                     /translation="MKMKWSPSVQGFFSENNSDIPDDAFDIEDALYYELMNGQSTGKI
                     IINNPDNYPVLTEYPAKTQEQEIAEAEGMKSILIEQANEYMNSKQWPGKAAIGRLKGE
                     ELAQYNLWLDYLDALELVDTSSAPDIEWPTPPAVQAR"
     gene            complement(741..1343)
                     /gene="tfaE"
                     /locus_tag="ACHKYO_RS13635"
                     /old_locus_tag="pgaptmp_002727"
     CDS             complement(741..1343)
                     /gene="tfaE"
                     /locus_tag="ACHKYO_RS13635"
                     /old_locus_tag="pgaptmp_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4429549.1"
                     /translation="MNKAVLNSELITTKAGDITVYNYDGETREYISTSTEYLAVGVGI
                     PACSCLDAPGTHKAGYAICRSADLNSWEYVPDHRGEVVYNTETGESKEITAPGNYPEN
                     TTTIVPLTPYDKWDGEKWVTDTEAQHSAAVEAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(1343..2887)
                     /locus_tag="ACHKYO_RS13640"
                     /old_locus_tag="pgaptmp_002728"
     CDS             complement(1343..2887)
                     /locus_tag="ACHKYO_RS13640"
                     /old_locus_tag="pgaptmp_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4429550.1"
                     /translation="MSTKFYTLLTDIGAAKLASAAALGVPLKITHMAVGDGGGVLPTP
                     DAKQTALVNEKRRAALNMLYIDPQNSSQIIAEQVIPENEGGWWIREVGLFDESGALIA
                     VGNCPESYKPQLAEGSGRTQTVRMVLITSSTDNIILKIDPAVVLATRKYVDDKVLELK
                     LYVDDQMRNHIAAQDPHTQYAQKHNPTFTGEPKAPTPAAGNNTTRIATTEFVQAAITA
                     LINGAPATLDTLKEIAAAINNDPKFSTTINNALALKAPLSSPALTGTPTAPTAAQSVN
                     NTQIATTAFVKSAIAGMVGSAPAALDTLNELAAALGNDPNFATTMLNALAGKQPLDNT
                     LTNLSGKDVAGLLAYLGLGEGSALPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELA
                     KAYPTNKLPDLRGEFIRGWDDERGVDSGRTLLSAQGDAIRNITGGFGQLRVNSEINAI
                     VDVQSVSGAFYGGTSVRNNINVSMTYANDRKIRQDVHFSAANVVPTANENRPRNIAFN
                     YIVRAA"
     gene            complement(2884..3489)
                     /locus_tag="ACHKYO_RS13645"
                     /old_locus_tag="pgaptmp_002729"
     CDS             complement(2884..3489)
                     /locus_tag="ACHKYO_RS13645"
                     /old_locus_tag="pgaptmp_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HGU4429551.1"
                     /translation="MNSLLPPGSTPLERRLARTCSGISDLQVPLRDLWNPATCPVSFL
                     PYLAWAFSVERWDESWTESVKRQVVKDAFYIHQHKGTTSAVRRVVEPFGFLIRIIEWW
                     QTGETPGTFRLDIGVQDQGITEDTYLELERLISDAKPCSRHMIGMSINLQTSGPHWVG
                     AASYLGEEITIYPYINETIISGGTAHEGGAVHVIDTMRVNP"
     gene            complement(3482..4390)
                     /locus_tag="ACHKYO_RS13650"
                     /old_locus_tag="pgaptmp_002730"
     CDS             complement(3482..4390)
                     /locus_tag="ACHKYO_RS13650"
                     /old_locus_tag="pgaptmp_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HGU4429552.1"
                     /translation="MAVIDLSQLPAPQIVDVPDFEALLAERKAEFVALHPKDEQEAVI
                     RTLELESEPVTKLLQENAYRELLLRQRINEAAQAVMVAYAMGGDLDQLAANYNVKRLT
                     VTPADNDAVPPVAAVMESDEALRLRVPAAFEGLSVAGPTAAYEFHARSADGRVADASA
                     TSPAPAEVVLTVLSREGDGTAEKDLLDVVENALNSENVRPVADRLTVRSAEIIPYRVE
                     ATIFLYPGPEAEPVMAAAKASLQKYIASQTRLGRDIRRSAIFAALHVEGVQRVELASP
                     LADVVLNKTQAASCTQWSVTNGGTDE"
     gene            complement(4377..4736)
                     /locus_tag="ACHKYO_RS13655"
                     /old_locus_tag="pgaptmp_002731"
     CDS             complement(4377..4736)
                     /locus_tag="ACHKYO_RS13655"
                     /old_locus_tag="pgaptmp_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HGU4429553.1"
                     /translation="MTLYSGMNNTSGKVITDIDHLRQSVRDILLTPQGSRIARREYGS
                     LLSALIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITINSNFDGSMVVELTGRRNN
                     GVPVSLSVSTGAENGSD"
     gene            complement(4733..5311)
                     /locus_tag="ACHKYO_RS13660"
                     /old_locus_tag="pgaptmp_002732"
     CDS             complement(4733..5311)
                     /locus_tag="ACHKYO_RS13660"
                     /old_locus_tag="pgaptmp_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU4429554.1"
                     /translation="MNAQLTEIMRLITNLIRTGVVTEVDREHWLCRVKTGDLETNWIN
                     WLTLRAGNARTWWKPSEGEQVVLLSLGGNLETAFALPAVYSNQFAPPSTSADACVTEH
                     PDGGWFEYEPATGRWYVRGIKSMVIEAADNITMKTSEFVLEADRTRINSEVVINGGVT
                     QGGGAMSSNGIVVDAHQHTGVLKGGDTTGGPV"
     gene            complement(5380..5826)
                     /locus_tag="ACHKYO_RS13665"
                     /old_locus_tag="pgaptmp_002733"
     CDS             complement(5380..5826)
                     /locus_tag="ACHKYO_RS13665"
                     /old_locus_tag="pgaptmp_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HGU4429555.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGRIKRQMFTKLRTTKYLKTAASEDSASVQFEGKVQRIA
                     RVHHYGLRDRVSRKGPEVRYAERRLLGVNDDVEAMTRDMILQWLAG"
     gene            complement(5819..6250)
                     /locus_tag="ACHKYO_RS13670"
                     /old_locus_tag="pgaptmp_002734"
     CDS             complement(5819..6250)
                     /locus_tag="ACHKYO_RS13670"
                     /old_locus_tag="pgaptmp_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4429556.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVATGASSMSW
                     EYRYTLNAVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSSVEAIAEPDEPEEMWTVKRG"
     gene            complement(6213..6461)
                     /gene="lysC"
                     /locus_tag="ACHKYO_RS13675"
                     /old_locus_tag="pgaptmp_002735"
     CDS             complement(6213..6461)
                     /gene="lysC"
                     /locus_tag="ACHKYO_RS13675"
                     /old_locus_tag="pgaptmp_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461641.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HGU4429557.1"
                     /translation="MLTLSGCGNAPHSPEVQLTVSGCPRVTPCRLDQAAPRTNGDLNA
                     LLDETEAAWAVCADKVDMIIACQERNSEQTTIPAPRPQ"
     gene            complement(6346..6774)
                     /gene="lysB"
                     /locus_tag="ACHKYO_RS13680"
                     /old_locus_tag="pgaptmp_002736"
     CDS             complement(6346..6774)
                     /gene="lysB"
                     /locus_tag="ACHKYO_RS13680"
                     /old_locus_tag="pgaptmp_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HGU4429558.1"
                     /translation="MNRLLLVVLALLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSALLRQRQHRIEELKRENEDLRRWADT
                     PLPADIIRLRERPTLTGGAAYRQWLSASDAVSAGSGSAAH"
     gene            complement(6771..7148)
                     /locus_tag="ACHKYO_RS13685"
                     /old_locus_tag="pgaptmp_002737"
     CDS             complement(6771..7148)
                     /locus_tag="ACHKYO_RS13685"
                     /old_locus_tag="pgaptmp_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HGU4429559.1"
                     /translation="MKKKLISGLFLMLWMALLIAAMVYPQGIFPVLAASGVWVACLLT
                     WAVIPVALVALIKNGPLWQELRASLLKTITRKENVFTSWVMRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITRNQMAQQVAA"
     gene            complement(7150..7662)
                     /locus_tag="ACHKYO_RS13690"
                     /old_locus_tag="pgaptmp_002738"
     CDS             complement(7150..7662)
                     /locus_tag="ACHKYO_RS13690"
                     /old_locus_tag="pgaptmp_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4429560.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALERCVKQQPPQK
                     VYDATVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            complement(7643..7858)
                     /locus_tag="ACHKYO_RS13695"
                     /old_locus_tag="pgaptmp_002739"
     CDS             complement(7643..7858)
                     /locus_tag="ACHKYO_RS13695"
                     /old_locus_tag="pgaptmp_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP1 family phage holin"
                     /protein_id="HGU4429561.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            complement(7862..8065)
                     /locus_tag="ACHKYO_RS13700"
                     /old_locus_tag="pgaptmp_002740"
     CDS             complement(7862..8065)
                     /locus_tag="ACHKYO_RS13700"
                     /old_locus_tag="pgaptmp_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HGU4429562.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            complement(8065..8529)
                     /locus_tag="ACHKYO_RS13705"
                     /old_locus_tag="pgaptmp_002741"
     CDS             complement(8065..8529)
                     /locus_tag="ACHKYO_RS13705"
                     /old_locus_tag="pgaptmp_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HGU4429563.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYASLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERYQDYDATASGVKRGEELAEASGDLWRDARWAISRVQDAPHCTVELI"
     gene            complement(8625..9275)
                     /locus_tag="ACHKYO_RS13710"
                     /old_locus_tag="pgaptmp_002742"
     CDS             complement(8625..9275)
                     /locus_tag="ACHKYO_RS13710"
                     /old_locus_tag="pgaptmp_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HGU4429564.1"
                     /translation="MSLSPARQHRLRVQAEQAAREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLAEEMSDAAQSAMLAATGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            complement(9279..10337)
                     /locus_tag="ACHKYO_RS13715"
                     /old_locus_tag="pgaptmp_002743"
     CDS             complement(9279..10337)
                     /locus_tag="ACHKYO_RS13715"
                     /old_locus_tag="pgaptmp_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HGU4429565.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRNAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQIGDFSAAAAETGA"
     gene            complement(10354..11187)
                     /locus_tag="ACHKYO_RS13720"
                     /old_locus_tag="pgaptmp_002744"
     CDS             complement(10354..11187)
                     /locus_tag="ACHKYO_RS13720"
                     /old_locus_tag="pgaptmp_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HGU4429566.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTSMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEIQEDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSKLETALQAQQAAFNELVDKLSHEDCRQDYRQRATGGNAPADTLTN
                     C"
     gene            11330..13096
                     /locus_tag="ACHKYO_RS13725"
                     /old_locus_tag="pgaptmp_002745"
     CDS             11330..13096
                     /locus_tag="ACHKYO_RS13725"
                     /old_locus_tag="pgaptmp_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HGU4429567.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDEQIEKLEEIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEMEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGLLCADGQYRQIVT
                     VEDAVRGGCNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            13096..14130
                     /locus_tag="ACHKYO_RS13730"
                     /old_locus_tag="pgaptmp_002746"
     CDS             13096..14130
                     /locus_tag="ACHKYO_RS13730"
                     /old_locus_tag="pgaptmp_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4429568.1"
                     /translation="MGKSKKNRAAATNQIQHKNQTSAEAFSFGDPVPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILTSTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRRKYYINGSHAGFIMYMTDAAQ
                     NQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGLQIIPLSEVAAKDEFLNIKNVSR
                     DDMMAAHRVPPQMMGIMPNNVGGFGDVEKASHVFVRNELIPLQKRLIELNEWLNDEII
                     RFDEYKLGNT"
     gene            complement(14168..15016)
                     /locus_tag="ACHKYO_RS13735"
                     /old_locus_tag="pgaptmp_002747"
     CDS             complement(14168..15016)
                     /locus_tag="ACHKYO_RS13735"
                     /old_locus_tag="pgaptmp_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429569.1"
                     /translation="MLVRGTSWRQGHVLKHDDAVSLGILQPDETNHKVVVITHDCDLQ
                     SNSEKNVELMFGPLKKGSSRMKRAKHPRILDLCFENPESAKKNAVELRHERKVIIPKE
                     AFCCEENDPAFSISTEEKQALKQWLAAKYGRPAFPNSFEERLRAYDEDKKFIFEKEVA
                     DIIATNAEHLIGVFFDLGEERFNDLEEGIPYELSINVVYDATEGGPDARRAAEQTCSD
                     LKALFYKFYGDPTLGHSELIELNTCIAVADTHFSLYALRRMDQWRVEYISLEEDSYGD
                     FIGAGV"
     gene            complement(15026..15688)
                     /locus_tag="ACHKYO_RS13740"
                     /old_locus_tag="pgaptmp_002748"
     CDS             complement(15026..15688)
                     /locus_tag="ACHKYO_RS13740"
                     /old_locus_tag="pgaptmp_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4429570.1"
                     /translation="MYAIPTGSIGSVDRYSPNLQSSSCVSGVVAVAASLLLVGTGASY
                     PVNAYKQWRQYVQPRVQFAFDSIESTYTPAISPEVDVRNVAQHLANIREVLSPSMSEL
                     AKDLGITRQALYKWLSGENQPDDASKVQFITNLSNVADSFSKAGLHDAKLLVKMKAFN
                     GKSLMDVVKEGEDWNRAVQVLIDEAKAMNAAAESANYLASKAKPTDDWKSSISIPGTV
                     EE"
     gene            complement(15708..16517)
                     /locus_tag="ACHKYO_RS13745"
                     /old_locus_tag="pgaptmp_002749"
     CDS             complement(15708..16517)
                     /locus_tag="ACHKYO_RS13745"
                     /old_locus_tag="pgaptmp_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HGU4429571.1"
                     /translation="MSTNSERMSNAPVYYALVQVKFTPIAAMSKYVPDIQDALRVEGF
                     PLFEVSNTTQLKFEIKNPNEPPVHSFEPVTSWLMIDADRRSGFVLGNDFITFHTTYYD
                     NHVSFISSLILGLSKVLEFAKPSLLSRIGLRYLDAVFPEKSETIEQYLVKELHGVDFG
                     WTPIQSIQESVYQTCVEPLIPNGFMVSRIHKMNGQLGFPPDMIPNGLLPLPRFSNTEH
                     RMHAIIDTDHYVEGNMSTDLQLIEKQILSLHSKVKEAFEGMVSDFARTKWH"
     gene            complement(16881..17114)
                     /locus_tag="ACHKYO_RS13750"
                     /old_locus_tag="pgaptmp_002750"
     CDS             complement(16881..17114)
                     /locus_tag="ACHKYO_RS13750"
                     /old_locus_tag="pgaptmp_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4429572.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(17125..17313)
                     /locus_tag="ACHKYO_RS13755"
                     /old_locus_tag="pgaptmp_002751"
     CDS             complement(17125..17313)
                     /locus_tag="ACHKYO_RS13755"
                     /old_locus_tag="pgaptmp_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429573.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRSHEYNRSMDVIS"
     gene            complement(17467..19881)
                     /locus_tag="ACHKYO_RS13760"
                     /old_locus_tag="pgaptmp_002752"
     CDS             complement(17467..19881)
                     /locus_tag="ACHKYO_RS13760"
                     /old_locus_tag="pgaptmp_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HGU4429574.1"
                     /translation="MSHADMNNCSGFNEVAAAFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYATDNEQEQLRREALSDQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQR
                     FNPGMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSTSFRFMASHEGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWWEEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCP
                     LAENLNQQGKDKSADGDTRTDITRMDDKELHDYLHSMSKKERRELAARLHLVKPKRCK
                     DYKQRITDHQRQQLVYELKSRGFDGSEKEVDLLLRGGSISSGAGLRIFYRNQRLQEDD
                     KWRNLY"
     gene            complement(19878..20735)
                     /locus_tag="ACHKYO_RS13765"
                     /old_locus_tag="pgaptmp_002753"
     CDS             complement(19878..20735)
                     /locus_tag="ACHKYO_RS13765"
                     /old_locus_tag="pgaptmp_002753"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - obsolete DNA methylation on
                     adenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HGU4429575.1"
                     /translation="MSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDY
                     PSYLVADINPDLINLYKKVAADCESFISRARVLFEIANREVAYYNIRQEFNYSTEITD
                     FMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKELRTFAEKAQRATFIC
                     ASFDETLAMLQVGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIV
                     SNSDTSLIRSLYRNFTHHYIKAKRSIGVAAGESKSATEIIAVSGPRCWVGFDPSRGVD
                     SSAVYGVRA"
     gene            complement(20732..21034)
                     /locus_tag="ACHKYO_RS13770"
                     /old_locus_tag="pgaptmp_002754"
     CDS             complement(20732..21034)
                     /locus_tag="ACHKYO_RS13770"
                     /old_locus_tag="pgaptmp_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HGU4429576.1"
                     /translation="MTGAVRIHQLKIAPKYFNAVVAGQKTAELRKDDRGYKVGDVLSL
                     CEWKHGVFTGREWAAVISHVLPVNDVMAVSEQWVMLSIRSLTPLEALGYVIAGGAV"
     gene            complement(21031..21258)
                     /locus_tag="ACHKYO_RS13775"
                     /old_locus_tag="pgaptmp_002755"
     CDS             complement(21031..21258)
                     /locus_tag="ACHKYO_RS13775"
                     /old_locus_tag="pgaptmp_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU4429577.1"
                     /translation="MADAMDLVQQRVEEERQSHIRAARAKTPGVSRVLCIECEAPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            complement(21258..21491)
                     /locus_tag="ACHKYO_RS13780"
                     /old_locus_tag="pgaptmp_002756"
     CDS             complement(21258..21491)
                     /locus_tag="ACHKYO_RS13780"
                     /old_locus_tag="pgaptmp_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HGU4429578.1"
                     /translation="MRNIEILTTKTGPDDAGLNILLTEARLEERRARAEAMAARLDSL
                     ACHISSRQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            complement(21559..21900)
                     /locus_tag="ACHKYO_RS13785"
                     /old_locus_tag="pgaptmp_002757"
     CDS             complement(21559..21900)
                     /locus_tag="ACHKYO_RS13785"
                     /old_locus_tag="pgaptmp_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HGU4429579.1"
                     /translation="MAINGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPKRLTMPN"
     gene            complement(22018..22314)
                     /locus_tag="ACHKYO_RS13790"
                     /old_locus_tag="pgaptmp_002758"
     CDS             complement(22018..22314)
                     /locus_tag="ACHKYO_RS13790"
                     /old_locus_tag="pgaptmp_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage filamentation protein Fil family protein"
                     /protein_id="HGU4429580.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWIMGEDGKRWHPCHSQDELL
                     SELTTRKRRKSKCMQRKVKWFISFVTEGRVIQYLKMICSVVIRRCRAMAVTFSR"
     gene            complement(22322..22831)
                     /locus_tag="ACHKYO_RS13795"
                     /old_locus_tag="pgaptmp_002759"
     CDS             complement(22322..22831)
                     /locus_tag="ACHKYO_RS13795"
                     /old_locus_tag="pgaptmp_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HGU4429581.1"
                     /translation="MFDYKISKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMVSAVDTVT
                     GLGASFGLL"
     gene            complement(22864..23106)
                     /locus_tag="ACHKYO_RS13800"
                     /old_locus_tag="pgaptmp_002760"
     CDS             complement(22864..23106)
                     /locus_tag="ACHKYO_RS13800"
                     /old_locus_tag="pgaptmp_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4429582.1"
                     /translation="MSTDISIRVPKEMATPAEFAEWEGISRGSVYQKIHHGQLAKYMV
                     KKEKNKGRVSLRYLMYKTDQVRESLGHSNFRVIVGK"
     gene            23228..23860
                     /locus_tag="ACHKYO_RS13805"
                     /old_locus_tag="pgaptmp_002761"
     CDS             23228..23860
                     /locus_tag="ACHKYO_RS13805"
                     /old_locus_tag="pgaptmp_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HGU4429583.1"
                     /translation="MLNIRMGSDTGGKAAIERLLEAYGFTTKQALSEHLNVSKSTMAN
                     RVLRDSFPADWIIQCALETGVSLLWLATGQGSMKEGYEPEKRSHNENKQAIKPLSKLI
                     TPSIPKGTLENGQLSIDEEIFLDHSILPADYEESMFLETPTDCYLIDKSVKQVSNGFW
                     LINIDGMIIIAKIMRIPGNKIVVNQDEASFECSADDVEVIGRAVKVIKSI"
     gene            23863..24879
                     /locus_tag="ACHKYO_RS13810"
                     /old_locus_tag="pgaptmp_002762"
     CDS             23863..24879
                     /locus_tag="ACHKYO_RS13810"
                     /old_locus_tag="pgaptmp_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGU4429584.1"
                     /translation="MTVRKQPNGKWLCECYPNGRNGKRVRKQFATKGEAIAFESFTME
                     EVNKKPWLGEKEDRRHLSELIEQWYSLYGQTLADPKRLIAKLRIICNGLGDPIASELT
                     AGDFTKYREARLKGEVRNEDGTFMSPVKPRTVNLEQRNLSSVFGTLKKLGHWSAPNPL
                     AGLPTFKIAESELAFLTPEEIKRLLDACADSQSSSLLLIAKICLATGARWSEAENLQS
                     HQVSKYRITYTKTKGKKNRTVPISKDLYEELPKNRGKLFTPCRKAFERAVKRAGIDLP
                     EGQCTHVLRHTFASHFMMNGGNILVLRDILGHSDIKMTMVYAHFAPDHLEDAVTKNPL
                     FNLR"
     gene            24890..25558
                     /locus_tag="ACHKYO_RS13815"
                     /old_locus_tag="pgaptmp_002763"
     CDS             24890..25558
                     /locus_tag="ACHKYO_RS13815"
                     /old_locus_tag="pgaptmp_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429585.1"
                     /translation="MNSELELLIKILFPSLSAIISLLGLKTGWTYKKDKLFTSRKNIS
                     EFSYQMYKSSEDPTFKKLAEDYGIAALTKDNTLTKKQRLILLNTTNPVSDIDDYSKCQ
                     SLISITTHKEIFAWNKKRYKYSIYRKLIKIITTLIYFMGSLIVALPFSYSVVVSAKMM
                     EKINHLTTWQYFGLSSYFVVSGVAICFICLDKLSKIKIAERLIVSNRRLGDKCSGGTL
                     AAES"
     gene            complement(25849..27118)
                     /locus_tag="ACHKYO_RS13820"
                     /old_locus_tag="pgaptmp_002764"
                     /pseudo
     CDS             complement(25849..27118)
                     /locus_tag="ACHKYO_RS13820"
                     /old_locus_tag="pgaptmp_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT         6571 a         7319 c         6429 g         6799 t
ORIGIN      
        1 aacggggtgt gggaacaggg gaaaatcaga taccggacat ggcttcattt agtggggtga
       61 gggattatta cggaaaacaa cttttgccag gagggttgat acttcagtgg ctgacgattc
      121 catcaagtgc agcagccaaa gctgtaacac tgaataatgg taattatcag ctgtcaggct
      181 ataaatggcc ccagtcattt ggtgtcctgt ttgctgtgtt tgctacaaaa gtttctggct
      241 cgactaacga agcatatgca atctcagtta atcgtcactc taccgatgta attgtcacct
      301 ggaatgcccg taaggctgat gatgtccaca ttttaggaat tgggaaatta tgaaaatgaa
      361 atggtcccca tctgttcagg gatttttctc tgaaaataac agcgatattc ccgatgatgc
      421 tttcgatatt gaagatgctc tttattatga acttatgaat ggtcagagta cggggaaaat
      481 tattatcaat aacccggata actaccctgt acttactgaa tatccagcga agacacagga
      541 acaggaaata gctgaagcgg agggaatgaa aagtatactt attgaacagg ccaacgaata
      601 catgaacagt aagcaatggc ctggtaaagc cgctattggt cgtctgaaag gtgaggaact
      661 ggcgcaatat aatttgtggc tggattatct ggacgcactg gaactggttg atacctccag
      721 tgctccagat attgaatggc ctacgcctcc ggcagttcag gccagatgac atccggcgca
      781 gtgctggtat ctgttgccgt caccgcgtca atgtaatcca gcacggcgtt aagtcgggtg
      841 gtttctgcct gcgtcagctt ccgcccggcc tgtaatttca gttgaatcag actgatggaa
      901 gccattgcag catcaatcag cgactggcgc tgtgcttctg ccgcttctac tgcggcgcta
      961 tgctgtgcct cggtatccgt cacccatttc tcaccatccc atttatcgta tggcgttaac
     1021 gggacgatag tggttgtatt ttcagggtaa ttacccggag ctgtgatttc ttttgattct
     1081 cccgtttcgg tgttatagac aacttcaccg cgatggtctg gcacatattc ccatgagttt
     1141 aaatctgccg aacggcagat tgcataacca gccttatgtg ttccaggagc atctaaacag
     1201 gaacatgccg ggataccgac acccacagca agatattcag ttgatgtgga aatatattct
     1261 cgtgtctcac catcataatt gtaaacggta atgtctcccg cctttgtggt gatgagttcg
     1321 ctatttaata cagctttatt catcatgcgg ccctcacaat ataattaaag gcgatgttac
     1381 gtggacgatt ttcatttgct gttggaacaa catttgccgc cgagaagtga acatcttgtc
     1441 ggatttttcg gtcattagcg tatgtcatag atacgttgat gttattccgg actgaggtac
     1501 ctccgtaaaa agcaccactc acactttgaa catcaacaat tgcatttatt tcgctgttta
     1561 cgcgcaactg accaaaaccg ccagtaatgt ttcgaatagc atctccttgc gctgaaagca
     1621 gagttcgccc actatccaca ccacgttcat catcccagcc acgaataaac tcaccacgta
     1681 aatcaggcaa tttatttgtc ggataagcct ttgccagttc cgggtattct tcagcagaaa
     1741 aagctgcacc gttgcatttc agccagcctg tcggcggtgt ggctgaaggc cacggaacag
     1801 gcaccccaac cggtaatgca gagccttctc ccaaaccaag gtatgcgaga agaccagcta
     1861 catcctttcc actcaaatta gtcagcgtat tgtccagcgg ttgtttacct gctagcgcat
     1921 taagcattgt cgtggcaaag ttcgggtcat tccccagcgc cgccgccagt tcgttcaatg
     1981 tatccagtgc cgcaggtgca gaacccacca ttcctgcaat cgccgatttc acaaaagccg
     2041 tagtggcaat ctgtgtattg ttgaccgact gcgccgcagt aggtgctgtt ggcgttccgg
     2101 taagtgccgg actcgacagc ggcgctttca gtgccagcgc attattaata gtggtactga
     2161 atttcgggtc attgttaatg gctgcggcaa tttctttcag tgtgtccagc gtggctggcg
     2221 caccgttaat cagagcagta atagcggcct gaacaaactc tgtggtcgca atccgagtgg
     2281 tgttatttcc tgctgcaggc gtcggcgctt ttggttctcc ggtaaatgtc ggattatgtt
     2341 tctgcgcata ctgggtatga ggatcttgtg cggcaatgtg gtttctcatc tggtcatcca
     2401 catacagctt taattccagg actttatcat ccacgtattt acgggttgcc agcactacag
     2461 cagggtcgat tttcaggatg atattgtccg tgctgctggt aatcagcacc atgcgcacgg
     2521 tctgggtacg cccgctgcct tcagccagtt gcggcttata gctttccggg cagtttccca
     2581 cggcaatcaa tgccccggac tcatcaaaca ggcccacttc acgtatccac caaccgccct
     2641 cgttttcagg gatcacctgt tcagcaataa tctggctact gttctgcggg tcgatataaa
     2701 gcatattcaa cgcagcccgg cgtttctcat ttaccagtgc cgtctgcttt gcgtccggcg
     2761 tcggtaatac tccgccgcca tcgccgaccg ccatatgggt aatttttagc ggcacaccga
     2821 gcgcggcggc gctggcaagt ttcgccgcgc caatatccgt cagcagggta taaaattttg
     2881 tgctcatgga ttcactctca ttgtgtcaat aacatggacc gccccgcctt catgcgcggt
     2941 gccgccagaa ataattgttt cgttgatata cggatagatc gtgatttctt caccaagata
     3001 gctggcggcc cctacccagt gcgggccact ggtctgcaga ttgatggaca tgccgatcat
     3061 gtgacggcta catggtttgg catcgcttat cagccgctca agttccagat aggtatcttc
     3121 agtgatgccc tgatcctgca cgccgatatc caggcgaaac gtgcctggtg tttctccggt
     3181 ctgccaccac tcaataatgc ggatcaggaa gccgaacggc tccaccaccc gccgcacggc
     3241 actggtggtt cctttgtgct gatgaatata aaaagcatcc ttcaccacct ggcgtttgac
     3301 gctttctgtc cagctctcgt cccagcgttc cacagagaac gcccaggcga gataaggcag
     3361 gaaactgacc ggacaggtcg caggattcca caagtcacgc agtggcacct gcagatcaga
     3421 aatcccgctg caggttcgcg ccagtcggcg ctccagtggt gttgaacccg gtggcagcag
     3481 actattcatc cgttcctccg ttggttacgc tccactgcgt acatgatgcc gcctgtgttt
     3541 tgttcaggac cacatccgcc agcggcgaag ccagctccac acgctgcacc ccctcaacat
     3601 gcagggcggc aaagatggcg ctacggcgaa tatcccgacc aagccgcgtc tgactggcga
     3661 tgtacttctg caggctggct tttgccgcgg ccattaccgg ctctgcttcc ggtcccggat
     3721 aaagaaaaat ggtggcttcc acgcgatacg ggatgatttc tgcgctgcga accgtcagac
     3781 ggtcagccac cgggcggacg ttctcactgt tcagggcgtt ctccaccaca tccagcaagt
     3841 ctttttctgc tgttccgtcg ccttcgcggc taaggacagt cagcaccacc tctgcaggtg
     3901 ccggactggt tgcactggca tccgccaccc gaccgtcggc gcttcgtgca tgaaactcat
     3961 aagcggcagt tggccccgca acagaaagcc cctcaaaggc tgcaggcaca cgcaggcgta
     4021 acgcttcatc gctttccatc acagctgcaa cgggcggcac agcgtcatta tcagcaggcg
     4081 tcaccgtcag gcgtttcacg ttgtagttgg cagcgagctg gtcaagatcg ccgcccatcg
     4141 cgtaagccac catcacagcc tgcgcggctt cgttaatgcg ctggcgcaga agcaactcac
     4201 ggtaagcgtt ctcctgaagc aatttggtga cgggttcaga ttccagttcc agcgtgcgga
     4261 tcacggcttc ctgctcatct ttcggatgaa gcgcaacaaa ttcggccttg cgttcggcaa
     4321 gcagcgcctc aaagtccggc acatcgacaa tctgcggcgc aggcaactgc gaaaggtcaa
     4381 tcactgccat tctctgctcc tgttgatacg gaaagggaca caggcacacc gttattccgc
     4441 cgcccggtca gctccaccac catagaaccg tcaaagttgc tgttgatggt gatggaatcc
     4501 agcgtcagcc gtggctccca gcgactcagc gccacataca ctgccgacat gacctgcagg
     4561 cgtaacgccg gattttgtgg ctggtctatc agtgccgaca gcagggaacc atattcacga
     4621 cgagcaatgc ggctaccctg cggcgtcagc agaatgtccc gcaccgactg gcgcagatgg
     4681 tcaatatcag taatgacttt gccgctggta ttgttcatcc cgctataaag cgtcataccg
     4741 gtcctccggt tgtatcgccg cctttcagga cgccagtatg ctgatgcgca tcaaccacga
     4801 tcccgttaga actcatcgct ccgccgccct gggtaacacc accattgatc accacttcgc
     4861 tgttaatgcg cgtgcggtca gcctccagta caaactcact ggttttcatg gtgatgttgt
     4921 cagcggcctc aatgaccatt gatttgatgc ccctgacata ccagcgcccg gtggcgggtt
     4981 cgtattcaaa ccagccaccg tcaggatgtt ctgtcacgca ggcgtctgcc gacgtcgacg
     5041 gtggtgcgaa ctgattcgaa tagacagcgg gcagcgcaaa ggcagtttcc agattgccgc
     5101 ccaaactcag tagcaccacc tgctcacctt ccgatggttt ccaccatgtg cgggcattac
     5161 ccgcgcgcag cgtcagccag ttaatccagt tggtttcaag gtcgcccgtt ttcacccgac
     5221 aaagccagtg ttcccggtcc acttcggtga ctacaccagt gcggatcaga ttggtgataa
     5281 ggcgcatgat ttcggttaat tgtgcgttca tagggaaagg ttgccatcag gggaagaaag
     5341 gcggcagtgc tgcaacttgt atcactgctg atacaaaaat caccccgcca accattgcag
     5401 aatcatgtcg cgggtcattg cctcaacatc atcatttacg cccagaaggc gacgctctgc
     5461 gtaacggacc tccggtcctt tgcggctgac gcgatcacgc aggccataat ggtgaacgcg
     5521 ggcaatgcgc tgcaccttac cttcaaactg cacgctggca gaatcctcgc tggcggcggt
     5581 tttcaggtat tttgtggtgc gaagttttgt aaacatctgc cgtttgatac ggcctttttt
     5641 actgcgtgct gttactcgcc tcggttcata gctgctgcca tcaggattgc gttgcatcct
     5701 gatgttctgc tgctgtgtcc ggcgcagttc ctgcgccagc tggcgcatca tgcggcttct
     5761 ggcggctggt tccagattcg ccagcaaggc actcagccag tcgtccactt tctgcagttc
     5821 agccacgttt caccgtccac atttcttcag gttcatcagg ttccgctata gcttcaacgc
     5881 tcgacacact accgtcagtg ctgaccagca cacgctccgt cagttgcagg ttcaggctga
     5941 tatcacagac atcgttgcgc agaatatcca cctcaaaggt gaatagtttt tcccgtaacg
     6001 ccgggttatt gatggcatcg ggctggttat ccctcagcca cagcaaaacc ggggccatca
     6061 gcagattctg gtcgccgctg aaatcctcaa tcaccgcgtt cagggtgtaa cggtactccc
     6121 acgacatgga gctggccccc gtggcaacca gcgaaccgtt atccacaaac agatgcagtt
     6181 tgtccgggtt attgcggaca taaggcaccg ctttattgag ggcgtggcgc agggattgtg
     6241 gtttgttcac tgtttcgctc ctgacacgca ataatcatat ccactttgtc tgcacagacc
     6301 gcccaggcgg cctccgtttc atccagcaac gcattcagat caccgttagt gcgcggcgct
     6361 gcctgatcca gccgacacgg cgtcactcgc ggacaaccac tgacggtaag ctgcacctcc
     6421 ggtgagtgtg gggcgttccc gcagccggat aatgtcagca ggcaaaggag tatcagccca
     6481 gcggcgtaaa tcctcgttct cacgtttcag ttcctcgatc cggtgttgtc gttgtctcag
     6541 cagcgcgctg gtctgttctg cttcggcata gagccgcgcc tgctcccggt tattggtttc
     6601 agtcagaata gacaggctga taagctggct gttgctcttt gccagtgcct ggcttttgct
     6661 ctgcagctcg tctgcctgcg tgctgatggt ctggctggca tcagccagcc gccacgtctg
     6721 ccagcccagc gccgccagta ataacgccag cacaaccagc agtaaccggt tcatgctgct
     6781 acctgttgcg ccatctgatt acgggtgatc cagaaggcaa taacggtcag tagataaaag
     6841 accagggtaa tagcccaccc cgtccaggcg agacttacaa caatcagcaa tcgcatcacc
     6901 caactggtaa atacgttttc ttttcgggta attgtcttca gcaaagatgc cctcaactcc
     6961 tgccagagcg ggccattctt aattaacgca accagtgcta ccggaattac cgcccatgtc
     7021 agcaaacagg ctacccaaac gccggacgct gccagtaccg gaaaaatccc ctgcggatac
     7081 accattgctg cgattaacag cgccatccat aacatcagaa acagtccgct gattaatttc
     7141 tttttcattt cagtttgctc cctgtaaaca ccaggccatc tcccgcgcac ggcggttatc
     7201 cagcccctga ttaaacacac cttttacata aacccagcgc ggcaactgtc ggcacgcatc
     7261 cgcccagcgc cgctgattga gcaatttcac cagcgtggag ctgcaggcat tgcccgtccc
     7321 cacgttgaag gcaaacgaca ccgtagcgtc atacaccttc tgtggtggct gttgcttcac
     7381 acacctttcc agtgcccgct ccacacgcag cacgttggag atcagccctt ctgctgcctg
     7441 tcgttccgtg attgttttgc cgggaatgac gcccgatgta ttaccaatgc cgtcggtcca
     7501 gacacccgcg ctgcactgat acggctgcag acgacagcct tcgtaatcgg caatcagttt
     7561 cagcccctcc acggaggtgt gaagctgctg aaaacccggc agcgtggcag caatagccag
     7621 cacggccccg acaaggcagc gtttaacgat tgatggattc atagtcctcc cgcgagatct
     7681 gcccgtcgcg cagaagctgg taggctttgt gtttgtagta ccagttgata gccagcatca
     7741 gcacaccaat catcaggccg cccagcgttg aggcatcctt gatggacaaa tcgcccagcc
     7801 aggccagcac gacggcgatg caatacgtga taaaggcgct gattcgctca agcgtcataa
     7861 ttcagtccca tagctggacg gtctgcacgg tggtggtggt cggaatgtcc ggcagctcca
     7921 cctgcagccc gtgaggtaaa aaggggccgt attcggcaag ccccggattt gccttcagta
     7981 cctgctccgt gacaccctgc gtgcgcccgt aatgacgcca gcaaagcgcg tccaccgtgt
     8041 catactgatg cgcacgcact ttcatcagat aagctccact gtgcagtgcg gcgcatcctg
     8101 cacccggctg atggcccagc gggcgtcacg ccacaaatca ccgcttgctt ccgccagttc
     8161 ctcgccccgc ttcacaccgg acgccgtggc gtcatagtcc tggtaacgtt cgttgagcat
     8221 ggcgcgtgcc cagcagtaaa ccgcgttgaa atagtgctga atgcgctcac ttttgccgtc
     8281 cagctgttcc gccggaacct ctgccagcga ggcatacccc agcatctgct ggcgtctgcg
     8341 aaactcatac agctctgcgt tgacctccga aattgccgat agcgcaacct gctttaaacg
     8401 cggctgcgtc accgtgccgt cagtgcgcat cacgctgcga aactccgaca ggtccacatc
     8461 aggccagaac ggcgtattcc tgatgatttc cgcctgttcc ggtgcctgtt ctggcgcaac
     8521 aaacttcatg ctgctttctc ctgaaataga gggcggtgga cggggttttg atgtggcagt
     8581 gcctttcgcc accccgtgcc gcccgtgcgc gggggcacgt tctgtcagcg gctgtcattg
     8641 cgcagtctgc gctccagctg ctgtttgtct tttttcacgc cacagcgggg atcgagctgt
     8701 aacgcatggt tgagatgatt aagggcggac gccggattgc tttcactcag gacagcgcca
     8761 atcgctttat gcagacgcgc ccgtgactgg tccggcatat ccagaccgtc tgtcagttcc
     8821 agcgtctgca gtaatagatc cgcatcaaag ccggtggcgg caagcattgc gctctgcgcc
     8881 gcgtctgaca tttcctctgc cagcacggtc tgcacgttgc ggttacccag cggcatcacc
     8941 cagccatgac gcagagcatg acgcccgatc tccagcgccc cggcataatc tccggcatca
     9001 atgcgccaca gcatcacgta catcagcacg tcatcctgtt gagcgcctcc ggcagccagg
     9061 acgccctccg cccaggcggc atatttcggc agtagctcca ccttgatttc cgcttttttc
     9121 accgtggact gaacgccctt gagacggcgg cggtcttccg ccagttgcag cagcatcagg
     9181 tcatagcccg atgcgtggcg aacactgccg ccctcgcggg cggcctgttc agcctgaacg
     9241 cgcaggcgat gctgccgtgc gggactcagg ctcatggatt acgctccggt ttcggctgcg
     9301 gcggcgctga aatcaccaat ctggatgttt tccaccagtg cggcacagcg gtagtcctca
     9361 accacatagg cttcgttaac ggattcaaag ttttcaatcc ggtcacgttt cgggttgtcg
     9421 ataactgaac ggcggcgggt gtcttcctgc cagtagatgg acaggttatc cagacgggtg
     9481 atcagcagcg cattcggcgg gaagaacggc gcacgcacgg cctgcaggcc acccatgcgt
     9541 ttctgactga tgatcatatc ggcagccagt ttttcactgt tttcctgctc tttgttgacc
     9601 agcgggaaat acttgtcaga cagcagttca cgaccgcaaa tcaccaccag atcgtcatcg
     9661 tcctggtaga ccacgtcgat aagctcattg acggcatcca tcaccacggc gtccaggttg
     9721 gcatattcgc cacctttccc gactttcact gcgcccggtg tggtttcacc gcccgtggtg
     9781 gtgctgccca tgacgtgatc cggtgcatcc tcacggattt tctgcagcca gcctttattc
     9841 acatcctgca gtagcgggtt ttcgctacgg ttagaggttt tcgcacgctt cacgccgtta
     9901 aagccgatca tgatgcggtc cagtgcctga cgtttcacga tggcgttacg gatacgcacc
     9961 tggaaatcct gaaacttcgc ccacaggtcc agcttcgcgt aggtcagcac cgtgtcaaag
    10021 ttggtctgtt cgcatttgta ttccacatcg accatcagcg tcggatcgac aggttcacgc
    10081 tctttcgcgg tggtgtcagt ggttccggca atggtgctgc caacacccag ccccagcagc
    10141 tgaccggact gctcagtcac tggcgtgacg ttaatcagcg tcaggaaagc ggcggactgc
    10201 tggatctggt cttccagcgt ctgctgcaca gacggctcca cagtgaactt gctggacagt
    10261 tcttcaactg ccacaccgtt cagacgcgcc agctgctgca ggtaagcgtt aaaagcaaag
    10321 cgggtattct tcttcatcgg gttttatgct ccatcagcaa ttggtcagag tgtcagcagg
    10381 ggcgttaccg cctgttgcac gctggcggta gtcctggcgg cagtcctcat ggctcagctt
    10441 gtccaccagt tcgttaaagg cggcctgttg tgcctgcagg gcagtctcca acttagacag
    10501 gcgttcttcc tgctcagaca gggatttttc ggtgcgtgcg ctcaggtttt gctgctcagt
    10561 ggcgaccagc tccacggcct tatgcacatc agagaaccgg gcatcgtcgg actgctcttt
    10621 tttggtgaac agcgccgtga cgcgggcaaa cagggacggc ttgtcctcct ggatttcttc
    10681 cagttcgatc accgtttcct ctgcggcggt aaagagattg gcgggattct gcttgcggtt
    10741 tgccagcggg ttatgggctg cactggcgct gaatgtcagc atttccgtac ccagactggc
    10801 tggatcatca gtggcggcca ggccaaccag gtaggctttg cccgtatcag cgaacttcgg
    10861 gctgacttcc atagaggtga ataatttctg gccttttttc accagttcca ccagggactc
    10921 cgttggctca acgtcggcat acagtgccat cttgcccgcc agcgggcctt ccgtgatttc
    10981 ttcagcaaac agcgccgtca ccttgccgta gcggttaaag gtgctgtccg gcagataaga
    11041 cttgatgtgc tcaaggttaa tcagcgcggt gtacaccgcc gggttgtagc tggctgccat
    11101 ctgttccagc cattcacgct ggatttcgcg tccgtcggtg gtggcacctt ccaccccgat
    11161 gcgaaaacgc tttgctttca ctgtcatgag ccgtgctccg ttagaaaaaa cttactggag
    11221 ccttatggtt gcggtgatgg gggcagtgaa acaatgcgcg gtatttgtac cgactaccac
    11281 acaaaccgca ggcggggaaa gccttcattc aaggctgtag gtttgtgcca tgaacaccac
    11341 actgacaccc gcagatctcg atccccgtcg gcaggccatg ctgctgtact ttcagggata
    11401 ccgtgtagcc cgcattgctg aaatgctggg cgagaaagtt gcaaccgttc acagctggaa
    11461 gaaacgcgac aagtggggtg actatgggcc gctggatcag atgcagctca ccaccgccgc
    11521 acgctactgc cagctcatta tgaaggagca caaagaaggg aaagatttca aagagattga
    11581 cctgctggcg cgccagtcgg aacgccacgc gcggatcggc aagtttaaca atggcggcaa
    11641 cgaagccgac ttaaacccta acgtcgccaa ccgcaacaaa ggcccgcgcc gtcagccgga
    11701 aaagaatgtt ttcaccgatg agcagattga gaagctggaa gaaatcttcc attcctccat
    11761 gttcaactac cagcgccact ggtgggaagc cggaaaaacc aaccgcatcc gcaacctgct
    11821 gaagtcacgc cagatcggcg cgacctttta ctttgcccgt gaagccctga ttgacgctct
    11881 gcttaccgga cgtaaccaga ttttcctttc cgccagcaag gcacaggccc acgtctttaa
    11941 gcagtacatc atcgacttcg ccaaagaaat ggaagtggag ctgaaaggcg atccgatggt
    12001 gcttcccaac ggggccacac tgtatttcct cggcaccaat gcccgcacgg cccagagtta
    12061 ccacggcaac ctgtatctgg atgaatattt ctggataccg aaattccagg agctgcgcaa
    12121 agtggcttcc ggtatggcta ttcacaaaaa atggcgacaa acctattttt ccacgccatc
    12181 cagcctgaca cacagtgctt atccgttctg gtccggtgcg ctattcaacc gtgggcgcaa
    12241 taaagccgac aaggtggaca tcgacctgtc ccacagcaat ctggctccag gcctgctgtg
    12301 tgcagacggg caataccgcc agatagtcac cgtggaagat gcggtgcgcg gcggctgtaa
    12361 cctgttcgac cttgaccagt tgcgcatgga atacagcccg gacgaatacc agaacctgct
    12421 gatgtgtgaa ttcgtggacg atctcgcgtc cgtatttccg ctcagcgagc tgcaggcatg
    12481 catggtggac agttgggaag tctggaccga ctttcatgca ctggccctgc gcccgtttgg
    12541 ctggcgagaa gtgtggatcg gttatgaccc ggcaaaaggt acgcagaacg gcgacagcgc
    12601 cggatgcgtg gtggtggcac cgccagccgt gccaggcggt aagtttcgca ttcttgagcg
    12661 tcaccagtgg cgcgggatgg acttccgcgc ccaggctgac gccatcaaaa aactgaccga
    12721 acagtacaac gtgacctata tcggtatcga ctcgacaggt gtcggtcacg gagtttacga
    12781 gaacgtgaaa gcgttctttc ctgccgtccg ggagtttgtc tacaacccca acgttaaaaa
    12841 tgccctggta ctcaaggcct acgacattat cagccaccgc cgtctggagt ttgacgccgg
    12901 gcacaccgac attgcgcagt cattcatggc aatccgtcgt gccaccaccg ccagtggcaa
    12961 ccgcccgacc tatgaagcca gccgcagcga agaagccagc cacgccgatc tggcctgggc
    13021 aacaatgcac gcactgttta acgaaccgct gcagggcgag tccgccaata ccagcaatat
    13081 tgtggagatt ttttgatggg aaagagtaag aaaaaccgcg ctgcggcgac gaatcagatc
    13141 cagcataaaa accagacttc agccgaagca ttcagcttcg gtgatcccgt tccagtactt
    13201 gaccgccgcg aactgctgga ctatgtggaa tgtgttcaga cagatcgctg gtatgagcct
    13261 cccgtcagct ttgacggact ggcgcgcacc ttccgcgctg ccgtgcatca cagttccccg
    13321 attgcagtaa agtgcaacat tctgaccagt acctatatcc ctcatccgct gctcagccaa
    13381 caggcttttt cgcgttttgt gcaggactat ctggtatttg gtaacgccta cctggagaaa
    13441 cgcacgaacc gattcggtga agttatcgcc cttgagcctg cgctggcaaa atacacccga
    13501 cgcgggttag acctggatac ctactggttt gtgcaatacg gtatgacaac ccagccgtat
    13561 cagttcacga aaggcagcat ttttcatctg atggaaccgg acatcaacca ggagatctac
    13621 ggcctgcccg gctatctttc tgccattcca tccgccctgc tcaacgaatc cgccacgctg
    13681 ttccgccgta agtattacat caacggtagt catgcaggct tcatcatgta catgaccgac
    13741 gccgcgcaga atcaggagga tgtgaacaac ctccgcaacg cgatgaaaag cgccaaaggt
    13801 ccaggcaact tccgcaatct gtttatgtac tcgcctaacg gcaaaaagga cggacttcag
    13861 attatcccgt tgtcagaagt cgcagcgaag gatgaatttc ttaacatcaa gaacgtgagt
    13921 cgggatgaca tgatggcagc gcaccgcgtg ccgccgcaaa tgatggggat tatgccgaat
    13981 aatgttgggg ggtttgggga tgtcgaaaag gcgagtcatg tatttgttcg taacgagtta
    14041 attccattac aaaaaaggct aattgaatta aatgaatggc ttaatgacga aattattcgt
    14101 tttgacgaat ataaattagg caatacttga ttaaagcctg tttaaactgg ctgtcggaac
    14161 agccagttta aacaccggca ccaatgaaat cgccatatga atcttcttca aggctgatgt
    14221 attctaccct ccattgatcc atccgcctca atgcatacag ggagaaatga gtatcagcaa
    14281 cagctatgca cgtattcaat tctataagtt ctgaatgccc caaggttgga tcaccataaa
    14341 acttataaaa caaagccttt agatcagaac atgtttgttc agccgctctt ctggcatcgg
    14401 ggccaccttc agttgcatca taaacaacat tgatagaaag ctcatatggt atgccttcct
    14461 caagatcgtt aaatctttct tcgcccaaat cgaaaaaaac tccgatcaaa tgctctgcgt
    14521 ttgtggcgat aatatccgca acttcttttt cgaaaataaa ctttttatcc tcgtcgtaag
    14581 cccgcaagcg ctcttcaaac gagttaggaa acgctggacg accatactta gcagctaacc
    14641 attgcttaag agcttgtttc tcttctgtgc taatggagaa tgctggatcg ttttcctcac
    14701 aacagaaagc ttctttagga atgatgactt tcctttcatg ccggagctct actgcattct
    14761 tcttcgcact ttctgggttt tcaaaacata gatcgagtat tctcggatgc ttagctcttt
    14821 tcatcctgct tgaccccttt ttcaaggggc caaacatcag ttccacgttt ttttctgaat
    14881 tactctgtaa atcacaatca tgcgttataa ctacaacttt atggttcgtt tcatcaggct
    14941 gcagaattcc aagtgaaacg gcatcatcat gtttaagtac atgcccctga cgccatgatg
    15001 ttcctcgtac caacataaat gtgatttatt cctctaccgt tccaggaatt gaaatagatg
    15061 acttccaatc atctgtcggt ttcgccttgc ttgctaagta attggccgat tcagcagcag
    15121 cattcatcgc tttagcttca tcaatcaata cctgaactgc cctattccaa tcctcacctt
    15181 cttttactac gtccattaat gacttaccat taaatgcttt cattttcacc aatagcttcg
    15241 catcatggag tccagctttg gaaaaagagt cagcgacatt actaaggttg gtaatgaatt
    15301 gcacttttga agcatcatca ggctggttct cccctgacaa ccatttatag agagcctgcc
    15361 gagttatccc caaatccttt gccagttctg acattgatgg agacagcact tctcgaatat
    15421 tagctaaatg ttgggcaaca ttcctgacat caacctcagg agaaattgca ggcgtgtacg
    15481 ttgattctat ggaatcaaat gcaaactgaa cccttggctg aacatactga cgccattgct
    15541 tataagcatt tacagggtat gaagcccccg taccaactaa tagtaaagat gcggcgacag
    15601 caacaacacc tgatacacat gagcttgact gcaaatttgg agaatatcga tctacagagc
    15661 ctatgcttcc ggtgggaatt gcatacattt tagtcacctc cgttgtttca gtgccattta
    15721 gtacgggcaa aatctgaaac catgccttca aatgcttctt taactttact atgcaacgaa
    15781 agaatctgtt tttcaattaa ctgcaaatca gttgacatgt tgccttcaac ataatgatct
    15841 gtatcaatta ttgcgtgcat acgatgctct gtgttactaa atctcggcaa cggcagtaaa
    15901 ccattaggaa tcatatctgg cggaaaacct agctgaccat tcatcttatg tattcgtgaa
    15961 accataaatc cattagggat caatggctca acacatgtct gatatacgga ctcttgtata
    16021 gactgtattg gcgtccatcc gaaatcaaca ccatgaagtt cctttacaag atactgctca
    16081 attgtttcgc ttttctcagg aaacacagca tccaaatatc gaagcccaat ccgacttaac
    16141 agcgatggct tagcaaattc tagaactttg ctcagaccaa gtatcaatga cgagatgaaa
    16201 gatacatggt tatcataata tgttgtatgg aacgtaatga aatcgttgcc taaaacaaaa
    16261 ccagatcttc tatctgcatc aatcatcaac caacttgtca caggctcaaa tgaatgtacc
    16321 ggtggctcat tggggttttt tatttcaaat ttcaactgag ttgtattgga aacctcaaat
    16381 aaaggaaatc cctcaactct tagcgcatct tgtatgtctg gaacatactt actcattgca
    16441 gctattggag taaactttac ctgtaccaat gcatagtaaa cgggcgcatt ggacatacgt
    16501 tcgctgttag tactcatgcc gccctccttg aaattgtttc atctcatatc tctttatctt
    16561 actaactgta gtttacacat aggttgacac tttctccatc attaagttca ctatgacatc
    16621 aatagccaaa actcttacgt aatcgcttcg gcgcgcgctc gtatccccgc cacgcctgcc
    16681 cgctttatgt agtggttttc atgcacctgc atgatctacg caaaagccca ccagttctgg
    16741 cgggccttag caaaaacgat cctcaaacga tcatgcgatc tcatgcggca tagacatgca
    16801 ctacagagct aacgcctcgc aagggctcgt tgttcaacct tgctgacgcc agaaacaagt
    16861 tcagacgcca gcaacgtttc ttaatgcagc cagctgtcgt cttcccacac cttctgcata
    16921 attttcatca cttgcttcct ttcttcgtcc agttgcagtc cggtcagttc cacaccgtta
    16981 gagctacctt tgcggatacg aattaccgtt ttgggataca gggggcgcag attacggtaa
    17041 agctcggatt caagggcgtc cagggtagac tggctaatct tctgctcttt atcgatcatt
    17101 atttcaatgc gcataaaagt cacctcagct gatgacatcc attgagcggt tgtattcgtg
    17161 gcttctgatt tttgccatga gttcatcagt caattcagaa acccactgca gagccagtcc
    17221 cttctcttca tcactacact cactggccgc tacaagctta agaaaaaaat caatgcgctg
    17281 gagcttcaaa gactccaaaa aatagtcctg catctttcct cctatgacac cacacgcaat
    17341 actgtatgta taaccactgt ttataattac agtatataat aatcttactg atgtaaaacg
    17401 tttttttacg tttatcagcc tgatatgcct ggtattatta agagcacgaa tggttaacca
    17461 tcgtaattaa tacaggtttc gccacttatc atcttcctgc aaacgctggt tccgatagaa
    17521 gatacgcagg cctgctcctg acgaaatact gccgccgcga aggagtaaat cgacctcttt
    17581 ctcgctacca tcaaaccctc tggacttcag ctcatacacg agctgctgtc gctgatggtc
    17641 tgtaattcgc tgtttgtagt ctttacaccg tttcggttta accaggtgta accttgctgc
    17701 cagttcccgg cgttcttttt tgctcatact gtgcaggtaa tcgtgcaact ccttgtcatc
    17761 catgcgggtg atatccgttc tggtatcccc atcagctgat ttgtctttcc cttgttggtt
    17821 caaattttca gcaaggggac agttattgcc acgagtccaa ggggcgcaag cgccctggtc
    17881 ggctgccgcc tcctgaacgt caacggcctt acgaaccatt ttccacttca cggcatgagt
    17941 gcagattttg ccctctgcaa tgggtgacca gatgccataa atacgaatgc cgtgatcgcc
    18001 ataggcggtc ggctcttcgt tgatttcata agcggttctg atgaggtgat atttacgggg
    18061 aaccagtacg ccgccctgct tcatgatgta ggtggcaaaa caaccagcat cagctgcagc
    18121 cagaatggca tcaaggcgcg ggttatccag taccggcgca cctgcttttt tgtcaccctg
    18181 ttgccttgcc gcctgaccag ccagcaatcg cagttcacgg taagcctgac gccccggaat
    18241 gccaaagaag cggaattgct gaacacgatg cagagacgcc caggcattaa cgtattcagc
    18301 gttatcacgc agagatttac ccgtttcctt gctgatctcg ccagccagac cacgcccgtc
    18361 aatgttctta ctgatgtatt tcgcgatgta gctagtcggc gttcctttgc gcgggtttat
    18421 cagctcagac ttaaagcgtg gccccgtgtt attacccagc tcctcgcggt cttcacggat
    18481 agcaaactta cgcaacaatg cagtaatggc gcggcggtct tttttgcgca tgaaacacaa
    18541 caggtgccag tgaactgtgc cgtcatgatg cggctcagcc acccgcacgc cataccagcg
    18601 caacccggct ttgtgcatcg ccttacgaaa tgcagcaaac atgccgacca gataatcact
    18661 gctttgtctt accgtcgcat ttgtccaggt cgggttgggc ctaccgttat ttagcgtgga
    18721 atggaaacgt gacggacagg tgatggtgta gaaaacggcg cagtcaccgc gcatttccgc
    18781 gataagctcc agacctttaa cacaggccat catctcattg cggcgatgcg cagggttgct
    18841 gctgctggcg tttaccacat cctccatgtc cagcgtgtcg ccgtcttcgt tcaccagttc
    18901 atgagaacgg aaaaactcca gcgacttacg gcgctgctca cgtttatgca tcacggcttc
    18961 atagctgaca tagggagatg cttttttgct gaccaggcaa acagcacgca actgctcttc
    19021 ccaccattcg caacgcatct tccacaattt ccgataccac cagtcggcgc acagcatacg
    19081 cgccagcgac cccggaatga gttcataggg cacgggttta cggcggtttc ttttccggcg
    19141 gagttgctca aacgcaggcg gtatgacatc cagtcgcagg gtttccgccg ccaccttttc
    19201 ccatgtcttg cggatttctt ctggcttaac gtcatcggtg gcatacaaat cgccacaagc
    19261 ggcatcaagg cacatgctca tatgcgcagc taccagggtg gacaggcgtt tcacctgatc
    19321 ctgactcatt tcaggcagga tcagcagacc gtccagccct tcatggcttg ccataaagcg
    19381 aaaagaagtg gatagctgac tgtcgcgtac atgctccagt cgttccagac atggcttaat
    19441 cgtctcacgc aaatagcggg aataagcctt tggcctgccc aggctgctga agtattcaat
    19501 acgttgcatc agcggcttgc tgatatggga aggctgggcg ttaacgtccg ccagaatgac
    19561 catgcccgga ttaaaacgct gctgctcatg cgccagcttt gcccggctaa tgagcttatc
    19621 ctgctccatt tcgcgctgga caggatcacg tgattcatta aagaaataac gctcccatac
    19681 ctgatcactc agagcctcgc ggcgcagttg ttcctgctcg ttatcggtag cgtacagagt
    19741 gatcaggttt gaaagcgcag aaaccggcgc aacttccgcc gggtccagat aagggttaat
    19801 ggcctttttc gggctgttcc atgagaatgc tgcggcgacc tcgttaaagc cgctgcagtt
    19861 gttcatatca gcatggctca tgcacgcact ccgtacacgg cagaactatc cacgccacgc
    19921 gaaggatcaa atcccaccca gcagcgcggc ccggaaacag cgatgatttc tgttgcagat
    19981 ttactctcac cagctgctac accgatgctg cgttttgcct tgatgtagtg gtgagtaaaa
    20041 ttgcgataca gcgaacggat cagggatgta tcactgttag aaacaatgac cggatgtcct
    20101 tctgatgacc gatgttcaag aacggatgcc aggtgatact ggtcatcttc agtgaaacca
    20161 tcagtgtgat agccggaaaa cgtaccgtca tacggcggat cgcaatacac cacatccccc
    20221 acctgcagca tcgccagcgt ttcatcaaag cttgcgcaga taaacgttgc tcgctgggct
    20281 ttttctgcaa atgtgcgaag ttctttttca gggaaatacg gatttttata attaccgtag
    20341 ggaatgttga aatgcccgct cttgttatag cgacataaac cacggtaacc gtgacgattg
    20401 agatacagga aatataccgc tttcatgaaa tcagtaattt cagtggagta attaaactcc
    20461 tgccttatgt tgtaataagc cacctccctg tttgcgatct caaataaaac tctggcgcga
    20521 gatataaacg attcacaatc agcggcaacc tttttataga ggttgattaa atcaggatta
    20581 atatccgcaa ccagatagct ggggtaatcc gtctccatca tcacagcaca agaacccgcg
    20641 aaaggttcaa ccagtcgcgg gcctgcagga aggtgttttt tcagttcgga cataatggcg
    20701 gttttattac ccgcccattt caggatggtg ctcatacagc acctcctgca ataacatatc
    20761 ctaaagcttc taatggggtt aatgagcgaa ttgatagcat cacccattgt tctgaaactg
    20821 ccatgacgtc attaaccgga agcacatgag agataaccgc ggcccattcc ctacccgtaa
    20881 atacgccatg cttccattcg caaagagaaa gaacatcacc aactttatag ccacgatcgt
    20941 ctttacgaag ttcagccgtc ttttgacctg caaccacagc gttgaaatac ttaggtgcaa
    21001 tttttaattg atggatacgc acagcccctg tcatacagca cctccgttgt aatgtttgcc
    21061 tttcagctct gcgatttcct ggcaagtaat gcaaagctgc acacccggaa tggcgcggcg
    21121 tcgtgctggc ggaattggcg cttcacactc aatgcaaagc acgcgagaca cgcccggtgt
    21181 tttggcacga gcagcacgga tatggctctg gcgttcttct tcaacgcgct gctgtacgag
    21241 atccattgca tcagccatta gtggatctcc tgcgcttcgt tctggattgc ttcagcagtc
    21301 acacgcagca gttctgccgc ttccacgtgg tttagctgac gggatgagat atgacacgcc
    21361 aggctatcaa ggcgagctgc cattgcttca gcccttgccc ggcgttcttc cagacgagcc
    21421 tctgtcagta aaatattaag ccctgcatca tccggtccgg ttttagtcgt aaggatttca
    21481 atattacgca taatcaattc tcctgaattt agataaaggg atgcccggcg ggtttacgcc
    21541 attaatttca ttagttggtt aattcggcat ggttagccgt ttgggaaata agctcaccac
    21601 tgcacgaaaa tgattcattg ctttaatcaa ctcccgcttt tcgtcagtgg tcagctcatt
    21661 aatgctgatg ctatgacgtt cagctggaat ttttgccata aagaatatgg cagccagtgc
    21721 tcgtttattt tgttcgttat tgatatcccg tggatcacgc atatctttaa taaaccgctc
    21781 aagctctgac tcaatattca ggccaaatac tttcgccctt aactccgcaa tgtgattaag
    21841 tccattcagg cgttcaccgg ggcttaatgg aacagtcgcc gcagcgccat taattgccat
    21901 aattcatatc cccaaaacgc aactatcgtt ctttgttctt acggtaacgt tcaagaggag
    21961 atacattttt tcgtatcgtc tctttaacct gctctccccg taaaaacgtc ccatccctta
    22021 gcgtgaaaaa gtaactgcca tcgcccgaca acgacggata acaacagagc aaatcatctt
    22081 caggtactga ataactctcc cctctgtaac gaaactgata aaccacttca ctttccgctg
    22141 catacatttt gactttctcc gtttcctcgt ggtcaattca gacagcaatt catcttgtga
    22201 atgacatgga tgccagcgtt taccatcctc acccatgatc cagccgtgac cgtagtgcat
    22261 tgccgggctt tgttttacca gcagcgatgc aaatgatggt tctttcgtca gcataagcac
    22321 ctcacagcaa accgaatgaa gcaccgaggc cagtcacggt atcaactgca ctcaccatcg
    22381 caggattagc ctgtaaacgg gcctgcaatg aaacagccgc cagcgccatc agtcgtgtta
    22441 cagagttaat gctgctgata gcatcacgac gaccggcact agtttttaca tcaccagata
    22501 ccgcacctgc tgcaacacgc ccgatctctg cagttgcgct catgacgtaa tgcggcagtt
    22561 tctcttttgc cacctcatta atcggtacac atggcagaca atgaatctgt gccagaaaac
    22621 catctaccag cgttgaatct tcagtcagat cggtaagcag ccagatttct ggtgcggtta
    22681 ataaatgagg ctgagctggg ttcagcttgt tccgcagaat ctgcacattc atgcctgcac
    22741 gttctgccag ttgcaccagg ttgtgacgca gtgcaaaagc cctacaggct tcatcgaaat
    22801 gcggatgttt ggaaatcttg taatcaaaca tggtgccccc ttagaaagtt cccataattg
    22861 aacttactta ccaacaatga cgcggaagtt ggaatgaccg agggattcac ggacctgatc
    22921 agttttgtac atcagataac gaaggcttac gcggccttta tttttttctt tcttgaccat
    22981 gtacttagca agttgaccat ggtgaatttt ttggtaaaca gagccacggg aaatgccctc
    23041 ccactctgcg aactctgcag gcgtagccat ctcttttggt acacgaattg aaatatcagt
    23101 actcatagtg cagtatctct tactttgtgt gcgtgttagt tcgttttagc tcgtctcttt
    23161 aactctcaca tcaagagaca cgaagacatt acgatcttga ttcaagattg tcaaatggag
    23221 attaccaatg ttaaacatca gaatgggttc cgatacggga ggtaaggcag ctattgagag
    23281 gctgcttgag gcttatggat tcacaactaa gcaggcatta agcgagcacc tgaatgtctc
    23341 aaaaagtact atggcaaaca gagtgttacg tgacagtttt cctgctgact ggataattca
    23401 gtgtgcacta gaaaccggtg tttcgttact ttggttagcc actggacagg gaagcatgaa
    23461 agaaggatac gagcctgaga aaagatctca taacgagaac aaacaagcaa ttaaaccgtt
    23521 atccaaactc ataactccat ctattcctaa aggaaccttg gaaaatggac aactcagtat
    23581 tgatgaagag attttcctag accacagcat attacctgca gattatgaag aatcgatgtt
    23641 cttagaaacc cctactgatt gttatctcat cgataaatca gttaaacaag tcagcaatgg
    23701 attctggctt atcaatattg atggaatgat tattattgca aaaatcatgc ggattcccgg
    23761 caataagatt gtagtaaatc aagatgaagc gtctttcgag tgctctgctg atgatgtgga
    23821 agttattggg cgtgcagtca aagtaataaa gagtatctaa acatgactgt cagaaaacag
    23881 ccaaacggta aatggttgtg cgagtgctat cccaatggac gcaatggcaa gcgcgtgcgt
    23941 aagcaatttg ctacgaaagg cgaagccatt gcttttgaaa gcttcacaat ggaagaagtg
    24001 aacaaaaaac catggctggg ggaaaaggaa gatcggcgac acctatcaga attaattgag
    24061 cagtggtatt ccctgtatgg tcaaacactc gcagacccca agcgcctcat agcgaaactt
    24121 agaattatct gtaatggtct aggcgatccc atcgcttcag aactgacagc cggtgacttt
    24181 acgaaatacc gcgaagcacg gttaaaaggt gaagtacgaa atgaagatgg cacgtttatg
    24241 tcgcccgtta agccccgcac ggtaaacctt gaacagcgca atctatcatc tgtttttggt
    24301 acactgaaaa agctgggcca ctggtcagcc cccaacccgc ttgctgggct gccaacattt
    24361 aaaattgctg agagtgaatt ggcgttcctg accccggaag aaattaaacg tctgctggat
    24421 gcctgcgccg attctcaaag ctccagcctg cttttgattg caaaaatatg cctggccacc
    24481 ggcgcacgct ggagcgaagc cgaaaacctg cagagtcatc aggtatcaaa ataccgtatc
    24541 acttatacca agaccaaagg caagaaaaac cgaactgtgc ctatttctaa ggatttatat
    24601 gaagaactgc ctaagaatag ggggaagtta ttcaccccat gcagaaaagc ctttgagcgt
    24661 gcagtaaagc gggctggtat cgatctgcca gagggtcaat gtactcacgt tctgcgtcat
    24721 acattcgcta gccattttat gatgaatggt ggaaacatat tggttttgcg ggatatccta
    24781 ggacattcag atataaaaat gaccatggtc tatgcacact tcgccccaga ccatttagaa
    24841 gatgccgtaa ctaaaaatcc tttatttaat ttgaggtaag tagtaattta tgaatagcga
    24901 actagagtta cttataaaaa tattattccc ttccctatct gccattatat ccttattagg
    24961 gcttaaaaca gggtggactt ataaaaaaga caaactattc acatcacgta aaaacattag
    25021 cgaattttca tatcagatgt ataaaagcag tgaagatccg accttcaaaa aactggcgga
    25081 agattacggc attgctgcat taaccaaaga taacacattg acaaaaaaac aaagattaat
    25141 attactcaac acaacaaacc ccgttagcga tatagatgat tattccaaat gtcaaagtct
    25201 aatttccatt accacccata aagaaatatt cgcatggaat aaaaaaagat ataaatacag
    25261 catatataga aaattaataa aaattataac aactctaatt tacttcatgg gcagtctcat
    25321 cgtagcactt cctttcagtt attctgttgt agtaagtgct aagatgatgg agaagataaa
    25381 ccatctaact acttggcaat actttggttt aagcagctat tttgtggtat cgggagtagc
    25441 aatatgcttt atctgcctag ataagctctc caagattaag atcgccgaga gattaatcgt
    25501 ttcaaatagg cgtttaggcg acaaatgcag tggcggcact ttggcggcag aaagttaaaa
    25561 acccataaaa cggacagaca ccgcataaat ctaaccaact gaaaatcaaa gcaaatcatt
    25621 gtttttacag acattgaaat gctatgtagg aatttcggac gcgggttcaa ctcccgccag
    25681 ctccaccaat catgattgga cggtataagg acaacaccaa taaaaacagg aagttagcag
    25741 tctcagcagg acaccgacca gacggtgaag agacataaaa ggatacgcaa aggagccgcg
    25801 gctcctggtg acatgaaagc ccacagatgt gggctttttc gttgatggtc agaacgacca
    25861 gttcacacca gctaccccgt tccacgggga ttccacaccg gcaccatggc tataccccac
    25921 cccaagatgc ccgcttaacg tactgctgaa tgaggcttta atacctgcct ggtatattcc
    25981 acgtctgccc gacaaatcat tgacgaaatt accgtcacta ttcactttca cccggttatc
    26041 atcgataaat tctttgcgca cagccgcctt cagccacggc tcaacttcca taccgttccc
    26101 cagacgcatg ttgtaactca gcgttgcacc cagttcacga tataaactgc gggtatcgac
    26161 tgatttcgat tccatgccat tggataaatg atattcaggg ttatcagcgg tgaacccccg
    26221 ttaacgatgc atacggcgtc aggttccagt taccatcggt aaatcgcatc ccggtttcaa
    26281 tgtgaccgcc cagcccgttg ctgcgataac tgccgttggc ggctccaccg ctgccaaccg
    26341 tggagtcgcg agcttcggta cctgctaata ccaataaatt accgccattt tccagcaaca
    26401 tattggtcgc taaattgccg gaaatggaaa aagtgccgta ctggtgagta ccgctgattt
    26461 caataccgtt agccgtgctc gtctggagag cggcaccgct gttctggacg atatctgtcg
    26521 ctttgccatt atcgttaact gtcagcgtac cgccttcatt gatctttgtt tttattgcct
    26581 ctccgttagc tgaaactgtt tgtattccgc cgtcgttaat cgttgtctca ttcgccaccc
    26641 cctcgacgat ttgttcgccg ccggtgagcg tcgtgcctgt cgcagtggct tttgttttga
    26701 cgatctcccg tccgcccgta ttgacctgtg ttttgtcaga agaggtgtct gactccatgg
    26761 ttaacacgcc gccatttgcc agcaggatat tgttcgccgc accctgctcg atgctgaacg
    26821 cgacgccatc cgcgcgtgtt cctgtgaccc gcgtcgccct ggtggttgca accaaagcgt
    26881 cctggctact ctgctgtatc cccgttgcgc tgcctttctc ccgcacatcg agtgtgccgc
    26941 cgtcattaag caccgagttt tcagccagac cgccctcatt aactacctgt gaacacccat
    27001 taataatgga accttccgct gtcccgtttg ccataatttg ttgtaggcca gagacgatat
    27061 cggtattgat tgccttacca taattctgaa cggtttgcgt gccaccattg atgtgtgt
//
LOCUS       DAZGAN020000059        26941 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGAN020000059 DAZGAN020000000
VERSION     DAZGAN020000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.059"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            109..516
                     /gene="gloA"
                     /locus_tag="ACHKYO_RS13825"
                     /old_locus_tag="pgaptmp_002765"
     CDS             109..516
                     /gene="gloA"
                     /locus_tag="ACHKYO_RS13825"
                     /old_locus_tag="pgaptmp_002765"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HGU4429586.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            619..1266
                     /gene="rnt"
                     /locus_tag="ACHKYO_RS13830"
                     /old_locus_tag="pgaptmp_002766"
     CDS             619..1266
                     /gene="rnt"
                     /locus_tag="ACHKYO_RS13830"
                     /old_locus_tag="pgaptmp_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HGU4429587.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            1359..5976
                     /locus_tag="ACHKYO_RS13835"
                     /old_locus_tag="pgaptmp_002767"
                     /pseudo
     CDS             1359..5976
                     /locus_tag="ACHKYO_RS13835"
                     /old_locus_tag="pgaptmp_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(6027..6374)
                     /gene="grxD"
                     /locus_tag="ACHKYO_RS13840"
                     /old_locus_tag="pgaptmp_002768"
     CDS             complement(6027..6374)
                     /gene="grxD"
                     /locus_tag="ACHKYO_RS13840"
                     /old_locus_tag="pgaptmp_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HGU4429588.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            6709..7524
                     /gene="mepH"
                     /locus_tag="ACHKYO_RS13845"
                     /old_locus_tag="pgaptmp_002769"
     CDS             6709..7524
                     /gene="mepH"
                     /locus_tag="ACHKYO_RS13845"
                     /old_locus_tag="pgaptmp_002769"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HGU4429589.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            7652..8233
                     /gene="sodB"
                     /locus_tag="ACHKYO_RS13850"
                     /old_locus_tag="pgaptmp_002770"
     CDS             7652..8233
                     /gene="sodB"
                     /locus_tag="ACHKYO_RS13850"
                     /old_locus_tag="pgaptmp_002770"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HGU4429590.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(8379..9548)
                     /locus_tag="ACHKYO_RS13855"
                     /old_locus_tag="pgaptmp_002771"
     CDS             complement(8379..9548)
                     /locus_tag="ACHKYO_RS13855"
                     /old_locus_tag="pgaptmp_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4429591.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(9714..9803)
                     /gene="cydH"
                     /locus_tag="ACHKYO_RS13860"
                     /old_locus_tag="pgaptmp_002772"
                     /gene_synonym="ynhF"
     CDS             complement(9714..9803)
                     /gene="cydH"
                     /locus_tag="ACHKYO_RS13860"
                     /old_locus_tag="pgaptmp_002772"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HGU4429592.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            10102..11127
                     /gene="purR"
                     /locus_tag="ACHKYO_RS13865"
                     /old_locus_tag="pgaptmp_002773"
     CDS             10102..11127
                     /gene="purR"
                     /locus_tag="ACHKYO_RS13865"
                     /old_locus_tag="pgaptmp_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HGU4429593.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(11124..12056)
                     /gene="punR"
                     /locus_tag="ACHKYO_RS13870"
                     /old_locus_tag="pgaptmp_002774"
     CDS             complement(11124..12056)
                     /gene="punR"
                     /locus_tag="ACHKYO_RS13870"
                     /old_locus_tag="pgaptmp_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HGU4429594.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            12169..13380
                     /gene="punC"
                     /locus_tag="ACHKYO_RS13875"
                     /old_locus_tag="pgaptmp_002775"
     CDS             12169..13380
                     /gene="punC"
                     /locus_tag="ACHKYO_RS13875"
                     /old_locus_tag="pgaptmp_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HGU4429595.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            13671..14819
                     /gene="cfa"
                     /locus_tag="ACHKYO_RS13880"
                     /old_locus_tag="pgaptmp_002776"
     CDS             13671..14819
                     /gene="cfa"
                     /locus_tag="ACHKYO_RS13880"
                     /old_locus_tag="pgaptmp_002776"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HGU4429596.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(14859..15500)
                     /gene="ribE"
                     /locus_tag="ACHKYO_RS13885"
                     /old_locus_tag="pgaptmp_002777"
     CDS             complement(14859..15500)
                     /gene="ribE"
                     /locus_tag="ACHKYO_RS13885"
                     /old_locus_tag="pgaptmp_002777"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGU4429597.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            15715..17088
                     /gene="mdtK"
                     /locus_tag="ACHKYO_RS13890"
                     /old_locus_tag="pgaptmp_002778"
     CDS             15715..17088
                     /gene="mdtK"
                     /locus_tag="ACHKYO_RS13890"
                     /old_locus_tag="pgaptmp_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HGU4429598.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(17129..18385)
                     /locus_tag="ACHKYO_RS13895"
                     /old_locus_tag="pgaptmp_002779"
     CDS             complement(17129..18385)
                     /locus_tag="ACHKYO_RS13895"
                     /old_locus_tag="pgaptmp_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGU4429599.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            18693..18769
                     /locus_tag="ACHKYO_RS13900"
                     /old_locus_tag="pgaptmp_002780"
     tRNA            18693..18769
                     /locus_tag="ACHKYO_RS13900"
                     /old_locus_tag="pgaptmp_002780"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18727..18729,aa:Val,seq:gac)
     gene            18774..18850
                     /locus_tag="ACHKYO_RS13905"
                     /old_locus_tag="pgaptmp_002781"
     tRNA            18774..18850
                     /locus_tag="ACHKYO_RS13905"
                     /old_locus_tag="pgaptmp_002781"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18808..18810,aa:Val,seq:gac)
     gene            18958..19263
                     /locus_tag="ACHKYO_RS13910"
                     /old_locus_tag="pgaptmp_002782"
     CDS             18958..19263
                     /locus_tag="ACHKYO_RS13910"
                     /old_locus_tag="pgaptmp_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HGU4429600.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTVRLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            19389..20993
                     /locus_tag="ACHKYO_RS13915"
                     /old_locus_tag="pgaptmp_002783"
     CDS             19389..20993
                     /locus_tag="ACHKYO_RS13915"
                     /old_locus_tag="pgaptmp_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HGU4429601.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(21005..21817)
                     /gene="ydhT"
                     /locus_tag="ACHKYO_RS13920"
                     /old_locus_tag="pgaptmp_002784"
     CDS             complement(21005..21817)
                     /gene="ydhT"
                     /locus_tag="ACHKYO_RS13920"
                     /old_locus_tag="pgaptmp_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HGU4429602.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(21821..22606)
                     /gene="phsC"
                     /locus_tag="ACHKYO_RS13925"
                     /old_locus_tag="pgaptmp_002785"
     CDS             complement(21821..22606)
                     /gene="phsC"
                     /locus_tag="ACHKYO_RS13925"
                     /old_locus_tag="pgaptmp_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HGU4429603.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFAPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(22603..23271)
                     /locus_tag="ACHKYO_RS13930"
                     /old_locus_tag="pgaptmp_002786"
     CDS             complement(22603..23271)
                     /locus_tag="ACHKYO_RS13930"
                     /old_locus_tag="pgaptmp_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU4429604.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(23335..23973)
                     /locus_tag="ACHKYO_RS13935"
                     /old_locus_tag="pgaptmp_002787"
     CDS             complement(23335..23973)
                     /locus_tag="ACHKYO_RS13935"
                     /old_locus_tag="pgaptmp_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HGU4429605.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(23986..26088)
                     /locus_tag="ACHKYO_RS13940"
                     /old_locus_tag="pgaptmp_002788"
     CDS             complement(23986..26088)
                     /locus_tag="ACHKYO_RS13940"
                     /old_locus_tag="pgaptmp_002788"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HGU4429606.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSGNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(26109..26735)
                     /locus_tag="ACHKYO_RS13945"
                     /old_locus_tag="pgaptmp_002789"
     CDS             complement(26109..26735)
                     /locus_tag="ACHKYO_RS13945"
                     /old_locus_tag="pgaptmp_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HGU4429607.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         6527 a         7110 c         6586 g         6718 t
ORIGIN      
        1 aggctatacc gattacccga cgttgtaatc caacattgcg agcggcgtaa agccgccgct
       61 atactaaaac aacattttga atctgttagc cattttgagg ataaaaagat gcgtcttctt
      121 cataccatgc tgcgcgttgg cgatctgcaa cgctccatcg atttttatac caaagtgctg
      181 ggcatgaaac tgctgcgtac cagcgaaaac ccggaataca aatactcact ggcgtttgtt
      241 ggctacggcc cggaaaccga agaagcggtg attgaactga cctacaactg gggcgtggat
      301 aaatacgaac tcggcactgc ttatggtcac atcgcgctta gcgtagataa cgccgctgaa
      361 gcgtgcgaaa aaatccgtca aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa
      421 ggcggtacta cggttatcgc gtttgtggaa gatccggacg gttacaaaat tgagttaatc
      481 gaagagaaag acgccggtcg cggtctgggc aactaatctc ctgccgggcg tgaactcatc
      541 gcgcccgcat ctttactgca tcgacaagta atatttgtca taatgcgcgc tgcaatttat
      601 ccgtattaag agaatcagat gtccgataac gctcaactta ccggtctgtg cgaccgtttt
      661 cgtggttttt atcctgttgt gatcgatgtt gaaacagccg gatttaacgc caaaaccgat
      721 gcgctgcttg agattgccgc catcaccctg aaaatggatg aacaaggctg gctgatgccg
      781 gacaccacat tacatttcca cgtcgaacca tttgtcggcg caaatttgca gccagaggcc
      841 ctcgccttca acggcattga cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag
      901 gcgctgcacg aaatttttaa agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc
      961 gccattatgg tggcgcacaa tgccaatttt gatcacagct ttatgatggc cgccgcagaa
     1021 cgcgcctcac tgaaacgtaa cccgttccac cctttcgcca cttttgacac tgctgcactg
     1081 gccgggctgg cactcggaca aaccgtattg tcaaaggctt gccagaccgc tggcatggac
     1141 ttcgacagca cccaggcgca ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt
     1201 tgtgaaatcg tcaaccgctg gaaacgtctg ggaggctggc cgctacctgc cgccgaagag
     1261 gtgtaatcga gtcgatgcct gatgacatgc aatgattcag gcatctatag tgaggctatt
     1321 ccacgcatca agcatgatat tcacggggaa tagcgttaat ggcagataat ccagaccctt
     1381 catcgctcct gccggacgtg ttttcaccgg cgatccgcga ctggtttctt cgcgccttta
     1441 aacagccgac cgctgtccag tcgcaaacct ggcatgtggc ggcgcgaagc gaacatgcgc
     1501 tggtgattgc accgaccggc tccgggaaaa cgctggcagc atttctctac gccctcgatc
     1561 ggctcttccg cgaaggcggc gaagataccc gcgaggcgca taagcgtaaa acctcacgca
     1621 tcctctatat ttcaccgata aaagctctgg gcaccgacgt tcagcgcaac ttgcagctcc
     1681 cgttgaaggg tattgccgat gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg
     1741 tagggatccg tactggcgat acgcctgcac aggaacgcag caaactcacc cgtaatccgc
     1801 cggatattct gatcaccaca cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg
     1861 aaacgctacg cggcgtcgaa acggtaatta ttgatgaagt ccacgcggta gcgggcagta
     1921 aacgtggtgc gcatctggcg ttaagtctgg agcggctcga tgcgctgctc cacacctcag
     1981 cacagcgaat tggcctttct gccactgtgc gctcagccag cgatgtggca gcatttcttg
     2041 gtggcgatcg cccggttacg gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa
     2101 ttgtcgtacc ggtcgccaat atggatgatg tctcatcggt cgccagcggc accggcgaag
     2161 acagccatgc cggccgggaa ggctccatct ggccatatat tgaaacgggt atccttgatg
     2221 aagtgttgcg ccatcgctcg accattgtct ttactaattc acgtgggctg gcggaaaaac
     2281 tgacggcacg attaaatgag ctttacgccg cacgcttaca gcgttccccg tctatcgccg
     2341 ttgatacggc ccatttcgag tcgacctccg gcgcaacctc taaccatgta caaagtagcg
     2401 acgtttttat tgcccgctca caccacggct ccgtctctaa agaacaacga gcaatcaccg
     2461 aacaggcgct gaaatcgggt gaattacgct gcgtggtcgc aacctccagt ctcgaactgg
     2521 ggattgatat gggcgcggtg gatctggtga ttcaggtggc aacgccgctt tctgttgcca
     2581 gtgggttaca acgcattggt cgcgccggac atcaggttgg cggtgtatct aaagggctgt
     2641 ttttccctcg tacccggcgt gacttagtcg attccgcagt cattgtagag tgtatgttcg
     2701 caggcaggct ggaaaacctg acaccgccgc ataatccact cgacgtcctt gcgcagcaaa
     2761 ccgttgccgc cgcggcgatg gatgcattac aggtagacga atggtactcc cgcgtacgcc
     2821 gtgccgcacc gtggaaagat ctgccaagac gtgtttttga cgccacgctg gatatgcttt
     2881 ccgggcgcta tccctctggc gatttttctg cttttcgccc caaactggtc tggaacaggg
     2941 agaccgggat attgaccgcc cgacctggcg ctcaattgtt ggcggttacc agcggcggca
     3001 ccattccgga tcgtggcatg tatagcgtgt tattacccga aggtgaagaa caggccggtt
     3061 cgcggcgggt gggtgaactg gatgaggaga tggtatatga gtcgcgggtg aacgacatta
     3121 tcactctcgg cgctacctca tggcggatcc agcaaatcac ccgcgatcag gtgattgtga
     3181 ctcctgctcc gggtcgttct gcccggctcc ccttctggcg tggtgaaggt aacggacgtc
     3241 cggctgaatt aggcgagatg atcggcgatt ttcttcattt actggcggat ggcgcgttct
     3301 tttccgggac tattcccccg tggctggcag aagaaaatac gaacgccaat attcaggggt
     3361 tgattgacga gcaacgcaac gcgacgggca tcgttccggg gagtcgccat ctggtcctcg
     3421 aacggtgccg tgatgaaatt ggcgactggc gtattatttt gcactctccc tatggaagac
     3481 gggtgcatga accctgggcg ctggcgattg ccgggcgaat acatgcccta tggggcgctg
     3541 acgcgtctgt ggtcgccagt gatgacggca ttgttgcgcg cattcctgac actgatggta
     3601 aattgcccga cgccgcgatt tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg
     3661 aggcggtagg cagctcggca cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat
     3721 tattaatgcc ggggcgcact ccgggccatc gcacccccgc tttggcaaca acggctgcgc
     3781 gccagtcagt tgctggaaat cgctcaggga tatccggatt ttccggtcat tctcgaaacc
     3841 ctacgcgaat gtctgcaaga tgtttatgat cttcccgcac tggaacgttt gatgcgtcgc
     3901 ctgaacggtg gcgaaattca aatatccgat gtaacgacca ctacgccctc gcctttcgcc
     3961 acaagtttat tgttcggcta tgtcgcggaa tttatgtacc agagcgacgc cccgctggca
     4021 gagcgccggg catccgtact gtcgctggac agcgagttac tgcgcaatct actcggacag
     4081 gtcgatccgg gggaattact cgacccgcag gtcattcgcc aggtggaaga agagttgcaa
     4141 cgactggctc ctggcagaag agcgaaaggt gaagaaggat tgttcgacct gctgcgcgaa
     4201 ctggggccaa tgaccgttga agacctggcg caacggcata caggcagcag tgaagaggtt
     4261 gcgtcgtatc tggaaaatct tcttgcagta aaacgaatct tcccagcgat gattagcgga
     4321 caggagcgtc ttgcctgtat ggatgatgcc gccaggctgc gtgatgccct cggcgtacga
     4381 ctaccagagt cattgccaga gatttattta catagagtca gttacccgct tcgcgatctc
     4441 tttctgcgct atctccgggc tcatgctctg gtcacggctg aacaactggc tcatgagttt
     4501 agtctcggta ttgccattgt cgaagagcag cttcagcaac tgcgtgaaca gggtctggtg
     4561 atgaatctgc aacaagacat ctgggtgagc gatgaagtat ttcgtcgtct gcgtttgcgc
     4621 tcgctgcaag ccgccagaga agcgacgcgt cccgttgcag ccacgaccta tgcgcgattg
     4681 ctgctggaac gtcagggcgt attacccgcc accgatggta gcccggcgct ctttgcctca
     4741 acatcgccag gcgtttatga gggcgtagat ggcgtgatgc gggtgatcga acagcttgcc
     4801 ggagtcggtt tacccgcctc actctgggaa agccagatcc tgcctgcccg cgtacgcgac
     4861 tattcgtcag aaatgctcga tgaattactg gcaaccggtg cggttatctg gtcggggcaa
     4921 aaaaaactgg gtgaagatga cggcctggtg gcactgcatc tacaggaata tgctgcagaa
     4981 tcgttcactc ccgccgaagc ggatcaggcg aatcgttcgg cgctgcaaca agcgatagtc
     5041 gctgttctgg ctgacggagg agcctggttt gcacaacaaa tcagccaacg gatacgcgac
     5101 aaaatcggcg aatcggttga tctctctgcc ctgcaagagg cgttatgggc gctggtctgg
     5161 caaggcgtca tcaccagcga catttgggca ccgttacgcg ccctcacccg cagcagttcc
     5221 aacgcacgca cctcaactcg ccgcagtcac cgggctcgtc gtggacgtcc tgtctatgcg
     5281 caacccgtct cgccgctggt atcttacaac acaccaaatc tggctggacg ctggtcgtta
     5341 ttgcaggtgg agccactaaa cgataccgaa aggatgctgg cgctggcgga aaatatgctc
     5401 gaccgctacg gcatcatcag tcgtcaggcg gtgatagccg aaaatatccc tggcgggttt
     5461 ccatcgatgc aaacgctttg tcgaagtatg gaagactccg ggcgaattat gcgaggtcgt
     5521 tttgtagaag gtctgggtgg cgcacaattc gctgaacgtc tgactattga ccgattgcgc
     5581 gatctggcga cacaagccac gcaaacgcgc cactatacac cagtggcgct ctctgccaac
     5641 gatccggcta atgtgtgggg aaatcttctg ccctggcctg cacatccggc aacgctggtt
     5701 ccaacgcgtc gggcgggtgc gctggtggtc gtttctggcg gcaaattgtt actctatctg
     5761 gcgcaaggtg gcaaaaaaat gctggtctgg caggaaaaag aggaattact cgccccagag
     5821 gttttccacg cgctgactac cgcactgcgt cgcgaaccac ggctgcgctt tacgctaaca
     5881 gaagtgaatg atctaccggt ccggcaaacg ccgatgttta cgctgctgcg tgaggcggga
     5941 ttttcaagtt cgccacaagg gctggattgg ggatagagaa aggactgacg gatgcccgtt
     6001 cgcatccgtc agtattgcag gacggattat tccgcgtccg gctcttcaga cttgtattta
     6061 gcggcagttt ctttgatcag ctgctgcagt tcgccacgct gatacatttc gatcacgata
     6121 tcacaaccgc cgaccagctc gccgtcaacc cacagttgcg ggaaggtcgg ccagttagca
     6181 tatttcggca gttccgcacg aatgtccgga ttctgcagaa tatcaacata ggcaaaacgt
     6241 tcgccacatg cggcaagcgc ctggactgcc tgggcagaga aaccgcagct cggcagtttc
     6301 ggtgaacctt tcatgtacag caggatcggg ttttcagcaa tctggcgttg gattttttcg
     6361 atagtggtgc tcattgtctt gcttcctcaa acttctttac ggcagtaatc tgacattgta
     6421 gcgggtcagt gcggcatcgg aaaataacat tttcatcacg cttttgctat tttatccctt
     6481 tgctctattt gttgcatttc aaatattcgt ttcttctctg atgcgcggca aactggcgta
     6541 tttataacca tttttagctt tcactgctgc atttttttgc gctcgccaac gaaacgtatt
     6601 ttttaacaat aaaagctatt aactttctct tcttctatgc attagaatca tcaagttttg
     6661 taaatcagac gcaggcatga tagacctgcc ttaacagagg gacgctcagt ggcgcggata
     6721 aaccgtattt cgatcacgct ctgtgcttta ctttttacca ccctgccttt aacgcctatg
     6781 gcccatgctt caaagcaagc cagggagagt tctgctacca ctcatatcac caaaaaagca
     6841 gataaaaaga aaagcacggc aaccaccaaa aagacccaga aaacagcgaa aaaagccgcc
     6901 agtaaaagta cgaccaaaag caaaaccgct tcttccgtta aaaaatcttc cattaccgct
     6961 tctaaaaacg ccaaaactcg cagcaaacac gccgtcaata aaacggcctc agccagcttt
     7021 accgaaaagt gtaccaaacg taagggctat aaatcgcatt gtgtgaaagt caaaaatgcc
     7081 gcgtcaggaa ctcttgccga cgcgcacaaa gcgaaggtgc aaaaagctac gaaagtggca
     7141 atgaataaac tgatgcagca aattggtaag ccatatcgtt ggggtggcag ctcaccgcgt
     7201 accggttttg attgcagcgg cctggtttat tacgcttata aagatttggt gaaaattcgt
     7261 attccgcgta cggcgaatga aatgtatcac ctgcgtgatg cagcgccaat cgaacgtagt
     7321 gaactgaaaa acggcgacct ggtctttttc cgtactcagg gacgcggcac agccgatcat
     7381 gtcggcgtgt atgtcggcaa cggcaaattt attcagtcac cgcgcacagg tcaggaaatt
     7441 caaatcactt ctctcagtga agactactgg cagcgccact atgttggcgc tcgtcgggta
     7501 atgaccccaa aaacacttcg ctaaaacttt accctgttgt tacggcaaca gggtaagttc
     7561 atcttttgtc tcacctttta atttgctacc ctatccatac gcacaataag gctattgtac
     7621 gtatgcaaat taataataaa ggagagtagc aatgtcattc gaattacctg cactaccata
     7681 tgctaaagat gctctggcac cgcacatttc tgcggaaact atcgagtatc actacggcaa
     7741 gcatcatcag acttatgtca ctaacctgaa caacctgatt aaaggtaccg cgtttgaagg
     7801 taaatcactg gaagagatta ttcgcagctc tgaaggtggc gtattcaaca acgcagctca
     7861 ggtctggaac catactttct actggaactg cctggcaccg aacgccggtg gcgaaccgac
     7921 tggaaaagtc gctgaagcta tcgccgcatc ttttggcagc tttgccgatt tcaaagcgca
     7981 gtttactgat gcagcgatca aaaactttgg ttctggctgg acctggctgg tgaaaaacag
     8041 cgatggcaaa ctggctatcg tttcaacctc taacgcgggt actccgctga ccaccgatgc
     8101 gactccgctg ctgaccgttg atgtctggga acacgcttat tacatcgact atcgcaatgc
     8161 acgtcctggc tatctggagc acttctgggc gctggtgaac tgggaattcg tagcgaaaaa
     8221 tctcgctgca taataactga tggcaaatgc agcattgcct gatgcgcttc gcttaccatg
     8281 ccaacgcgga tgaggcgtta acagcgcatt cggcaagata aatcgcacgt tgtcagcaac
     8341 tgtaacgcag aaggttatcc ttctgcgttt ttgtttaatt agctgttagc aacgcaaact
     8401 gtttcaggtt gttttctggc tgacataaac accagcaata atgccagtcc cgcgacaatc
     8461 gctcccatca ccggcacaaa gctgtatccc agcccagcgg aaattaccgc accaccagca
     8521 gctgctccca gcgcatttcc aagattaaag gcaccaatat tgactgatga agacagaccc
     8581 ggcgcttcac tggcgacacg catcacgcgc atctgtaacg gcggtacgac cgcaaaggtt
     8641 gctgcgcccc acaccaccat gctaatagct gcgccgaact cattgcgggc caggaacggg
     8701 attgccagca taatcaccat cagcagcaac aaaaagcctt tcaacgtgcc gttaactgaa
     8761 cgatctgcca gtttgccgcc gagatagtta ccgatagaga atccgacacc aatcagcacc
     8821 agcattgccg tgacgaacac tggtgttgcg tgggtaatac tttgtagtac cggagagata
     8881 taggtgtaga gagtaaacat tgcaccagct cccagtaccg tcgtcagcaa tgcagacagc
     8941 acctgcggac gcattaatac cgccagctct tttttcactt caggtcgtgc ccctgcacca
     9001 cctttaggta atgagaagaa cagacttacc attgaaatca ctcccagccc cgccgttgcc
     9061 agaaatgaca tccgccagcc gatggtttca cccaaccagg tcgccgccgg cacgccaccg
     9121 atatttgcca gggttaaccc cataaacata gtggcaactg cgctggcctg tttatgtttt
     9181 ggcaccacgc ttgcggccac gactgaaccc aaaccaaaaa atgctccgtg attcaggctg
     9241 gtcaaaatgc gtgaaagcat cagggtcata taatccggcg cgatggcgga aagtacgttg
     9301 ccgagcgtga aaattgccat caggaaaatc aacgcactgc ggcgggcacg atgagaaagt
     9361 agaagcgtca tcagcggcgc gccaaccatt acgccaactg cataggcact gattaacatt
     9421 ccggcagcgg gaatcgagac atccacaccg cgcgcaatga cgggcaacaa gcccattggc
     9481 gagaactccg ttgtcccgat accaaacgcg ccaatcgcca gcgccagcaa cggatagtta
     9541 attttcatgc cttatctcca cctcttcgcg tcattacgcg atattcatta aagtggcgaa
     9601 agcatgacag caatcacaaa aaaatgaaaa taacaaaaag agaaaacact tttgccattt
     9661 tgctaacaaa caggaaggag atgcgaggga gaacgcgctc cctcgagtgg aaatcagtgc
     9721 agcgcggcag tcaaacccac ggccacgatc aaaccgagga cgataatcgt tgttaccagt
     9781 gaaaatttaa ggtcggtgct catcaagttt tctccttttt tattaccaca caaatagtga
     9841 tattacgcat ttttacacac tgtgatgaaa aaatctcccg tcatttataa tgataagtgt
     9901 ttttaccact tccccttttc gtcaagatcg gccaaaattc cacgcttaca ctatttgcgt
     9961 actggccatt gaccccttcc tgacgctccg tgtcgttttt ccggcgtacc gcaacacttt
    10021 tgttgtgcgt aaggtgtgta aaggcaaacg tttaccttgc gattttgcag gagctgaagt
    10081 tagggtctgg agtgaaatgg aatggcaaca ataaaagatg tagcgaaacg agcaaacgtt
    10141 tccactacaa ctgtgtcaca cgtaatcaac aaaacacgtt tcgtcgctga agaaacgcgc
    10201 aacgccgtgt gggcagcgat taaagaatta cactactccc ctagcgcggt ggcgcgtagc
    10261 ctgaaggtta accacaccaa gtctatcggt ttgctggcga ccagcagcga agcggcctat
    10321 tttgccgaga tcattgaagc ggttgaaaaa aattgcttcc agaaaggtta caccctgatt
    10381 ctgggtaatg cgtggaacaa tcttgagaaa cagcgggctt atctgtcgat gatggcgcaa
    10441 aaacgcgtcg atggtctgct ggtgatgtgt tctgagtacc cagagccgtt gctggcgatg
    10501 ctggaagagt atcgccatat cccaatggtg gtgatggact ggggtgaagc aaaagctgac
    10561 ttcaccgatg cggtcattga taacgcgttc gaaggcggct acatggccgg gcgttatctg
    10621 attgaacgcg gtcaccgcga aatcggcgtc atccccggcc cgctggaacg taacaccggc
    10681 gcaggccgcc ttgccggttt tatgaaggcg atggaagaag cgatgatcaa ggtgccggaa
    10741 agctggattg ttcagggtga ctttgaaccc gaatctggtt atcgcgccat gcagcaaata
    10801 ctgtcgcagc cgcatcgccc tactgccgtc ttctgtggtg gcgatatcat ggcaatgggc
    10861 gcactttgtg ctgctgatga aatgggtctg cgcgtcccgc aggatgtttc gctgatcggt
    10921 tatgataacg tgcgcaacgc ccgctatttt acgccggcgc tgaccacgat ccaccagcca
    10981 aaagattcgc tgggtgaaac agcgttcaac atgctgttgg atcgtatcgt caacaaacgt
    11041 gaagaaccgc agtccattga agtgcatccg cgcttgattg aacgccgctc cgtggctgac
    11101 ggcccgttcc gcgactatcg tcgttaatca cccgttgcgg gagtctcttc cggctcccgc
    11161 agccactcct tattcagcgt ctcactatcg ccgagatact caagcagcca ggttaacgca
    11221 ggcgacatat cattttgctg ccatgtcaga caacatgccg aatccgggaa ggggttttcc
    11281 aattcaagtg ctacccactt cccctcatta agccacggtt tggcgaaatg tgttggcacc
    11341 atccctatgc ataatcctgc cgagatacag gttgccgatg attcccagtc aggcacgaca
    11401 actctttttt ggttatccag caaccaggta atacgtttag gtagcgttcg cgaggtgtct
    11461 tcgcgcacca acgacggcca gttgcgcaac gtatcatcgc tgaatgggcc atccatcaac
    11521 gccagcgggt ggtggctggc aacaacgcaa ctccagctta gcatccccat atcccggaag
    11581 gcataacgac cgcctaccgg aatcgcgcgt gttgcgccaa tcgccagttc cacgcgcccg
    11641 tcggaaagcg catcccagac accgttgaac acttcctgaa agacaagaag ttcgacatca
    11701 tcaaaatggc gataaaaatc aacgatcatc tgccgtgtac gttctggcct gacaatatta
    11761 tccactgcga tagctaactg accgcgccag ccgttcgcta tctgctgaca ttgctggcgg
    11821 gtgatctgca tttttttgac aacagagcgc ccttctttga gaaaccacgc tccagcagcg
    11881 gtcagctcca catcacggtg ccgtcgttca aagagcggca ccgccagcca ctcttccagc
    11941 tgacgcacgg tatagctgac cgcagaagga acgcgatgca gctcctgtgc cgcagcgcta
    12001 aaactaccat tacgcgctac cgcatcaaca acttcgagtg aatattctga ccacatagtc
    12061 tgcctgcaaa atttttgaaa ccagtcatca aatattaccg tttcacaaca ctaatttcac
    12121 tccctacact ttgcggcggt gtttaattga gagatttaga gaatatacat gcaacctggg
    12181 aaaagatttt tagtctggct ggcgggtttg agcgtactcg gttttctggc aaccgatatg
    12241 tatctgcctg ctttcgccgc catacaggcc gacctgcaaa cgcctgcgtc tgctgtcagt
    12301 gccagcctta gtctgttcct tgccggtttt gccgcagccc agcttctgtg ggggccactc
    12361 tccgaccgtt atggtcgtaa accggtgtta ttaatcggcc tgacaatttt tgcgttaggt
    12421 agtctgggga tgctgtgggt agaaaacgcc gctacgctgc tggtattgcg ttttgtacag
    12481 gctgtgggtg tctgcgccgc ggcggttatc tggcaagcgt tagtgacgga ttattatcct
    12541 tcacagaaag ttaaccgaat ttttgcgacc atcatgccgc tggtgggtct atctccggcc
    12601 ctggctcctc tgttaggaag ctggctgctg gtccattttt cctggcaggc gattttcgcc
    12661 accctgtttg ccattaccgt ggtgctgatt ctgcctattt tctggctcaa acccacgacg
    12721 aaggcccgta acaatagtca ggatggtctg acctttaccg acctgctacg ttctaaaacc
    12781 tatcgcggca acgtgctgat atacgcggcc tgttcagcca gtttttttgc atggctgacc
    12841 ggttcaccgt tcatccttag tgaaatgggc tacagcccgg cagttattgg tttaagttat
    12901 gtcccgcaaa ctatcgcgtt tctgattggt ggttatggct gtcgcgccgc gctgcagaaa
    12961 tggcaaggca agcagttatt accgtggttg ctggtgctgt ttgcagtcag cgtcattgcg
    13021 acctgggctg caggcttcat tagccatgtg tcgctggtcg aaatcctgat cccattctgt
    13081 gtgatggcga ttgccaatgg cgcgatctac ccgattgttg tcgcccaggc gctgcgtccc
    13141 ttcccacacg caactggtcg cgccgcagcg ttgcagaaca ctctacaact gggtctgtgc
    13201 ttcctcgcaa gtctggtagt ttcctggctg atcagtatca gcacgccatt gctcaccacc
    13261 accagcgtga tgttatcaac agtagtgctg gtcgctctgg gttacatgat gcaacgttgt
    13321 gaagaagttg gctgccagaa tcatggcaat gccgaagtcg ctcatagcga atcacactga
    13381 tctatatcga tatacttata cttaggctgc taacaaaatt ttgttgtatg attgaaatta
    13441 gcggcctata ctaatttcga gttgttaaag ctacgataaa tattatgttt ttacggggat
    13501 aggatcgttc ccgactcact atggatggtc atttcggcaa aggttcctcc tttccctctg
    13561 ttctacgtcg gattatagac tcgcggtttt ttctgcgaga tttctcacaa agcccaaaaa
    13621 gcgtctacgc tgttttaagg ttctgatcac cgaccagtga tggagaaact atgagttcat
    13681 cgtgtataga agaagtcagt gtaccggatg acaactggta ccgtatcgcc aacgaattac
    13741 ttagccgtgc cggtatagcc attaacggtt ctgccccggc ggatattcgt gtgaaaaacc
    13801 ccgatttttt taaacgcgtt ctgcaagaag gctctttggg gttaggcgaa agttatatgg
    13861 atggctggtg ggaatgtgac cgactggata tgttttttag caaagtctta cgcgcaggtc
    13921 tcgagaacca actcccccat catttcaaag acacgctgcg tattgccagc gctcgtctct
    13981 tcaatctgca gagtaaaaaa cgtgcctgga tagtcggcaa ggagcattac gatttgggta
    14041 atgacttgtt cagccgcatg cttgatccct tcatgcaata ttcctgcgct tactggaaag
    14101 atgccgataa tctggaatct gcccagcagg cgaagctcaa aatgatttgt gaaaaattgc
    14161 agttaaaacc agggatgcgc gtactggata ttggctgcgg ctggggcgga ctggcgcact
    14221 acatggcatc taactatgac gtaagcgtgg tcggcgtcac catttctgcc gaacagcaaa
    14281 aaatggctca ggaacgctgt gaaggcctgg atgtcaccat tttgctgcaa gattatcgtg
    14341 acctgaacga ccagtttgat cgtattgttt ctgtggggat gttcgagcac gtcggaccga
    14401 aaaattacga tacctatttt gcggtggtgg atcgtaattt gaaaccggaa ggtatattcc
    14461 tgctccatac tatcggttcg aaaaaaaccg atctgaatgt tgatccctgg attaataaat
    14521 atatttttcc gaacggttgc ctgccctctg tacgccagat tgctcaatcc agcgaacccc
    14581 acttcgtgat ggaagactgg cataacttcg gtgctgatta cgatactacg ttgatggcgt
    14641 ggtatgaacg attcctcgcc gcatggccag aaattgcgga taactacagt gaacgcttta
    14701 aacgaatgtt tacctattat ctgaatgcct gtgcaggtgc tttccgcgcc cgtgatattc
    14761 agctctggca ggtcgtgttc tcacgcggtg ttgaaaacgg ccttcgagtc gctcgctaaa
    14821 ggctattcta tcgccccctc tccgggggcg atttcagatc aggcttctgt acctggttga
    14881 ttcatggcat tttctcgtgc cgccagcaca cgttctaccg tatctaccac tgcctgagtt
    14941 tgtggatcaa tttcaatgtt gacgcgtgcg ccgagttttt tcttcccaag agtcgtgcgt
    15001 tccagagttt ccggaattaa atggacgcaa aaacgcgttg gcgtgacttc gccgacggtc
    15061 aggctaatac cgtcgatgcc aataaatcct ttgtacagga tatatttcat caactgacta
    15121 tcctggactt taaaccagat ctggcgatta ttttccgagg ttaatatttt cgccacttca
    15181 gcggtggtca taatatgacc tgacattaag tgtccgccaa tttcatcact gaatttcgcc
    15241 gcacgctcaa cgtttaccca atcccccact tttaaatcgc caagattggt aatgcgtaac
    15301 gtttctttca tcaggtcaaa actgacatgg ttgccgttaa tttccgtcac ggtcaggcag
    15361 caaccgttat gcgccacgga agcacctgtt tccaggccgt ccagcatgtg gtcgggtaac
    15421 tccaccacat gcgtacgaaa atttggtttc tcgtcaatcg acaccagttt tgcggtgccc
    15481 tgtacaatcc ccgtaaacat acttacaact cctgaaatca gttaagacat tctgttcagc
    15541 acaatagcag gtggcaaacg cccttaccag tgaaggggta agaatgacta ttttttcact
    15601 ggagaattaa taaatcctcg ctacaataga ctgaatttcc cctgcttctt ctttttgctg
    15661 cccattcagg cggcttttta gtctctcata taactacaaa taaaaggtgt tcacgtgcag
    15721 aagtatatca gtgaagcgcg tctgttatta gcattagcaa tcccggtgat tctcgcgcaa
    15781 atcgcccaaa ctgcgatggg ttttgtcgat accgtgatgg cgggcggcta tagtgccacc
    15841 gacatggcgg cggtcgctat cggtacttct atctggcttc cggcgatcct ctttggtcac
    15901 ggactgctgc tggcattaac gccggttatc gcgcaattaa atggttccgg tcgacgtgag
    15961 cgcattgcgc atcaggtgcg acaaggtttc tggctggcag gttttgtttc cgttctcatt
    16021 atgcttgtgc tgtggaatgc aggttacatt atccgctcca tggaaaacat cgatccggct
    16081 ctggcggaca aagccgtggg ttatctgcgt gcgttgttgt ggggcgcgcc gggctatctg
    16141 ttcttccagg ttgcccgtaa ccaatgtgaa ggtctggcaa aaaccaagcc gggtatggta
    16201 atgggcttta tcggcctgct ggtgaacatt ccggtgaact atatctttat ttatggtcat
    16261 ttcggtatgc ctgagctcgg tggcgttggt tgtggcgtgg ctactgcggc ggtgtattgg
    16321 gtcatgttcc ttgccatggt ttcttacatt aaacgcgccc gctccatgcg cgatattcgt
    16381 aacgaaaaag gcaccgcgaa acccgatcct gcggttatga aacgactgat tcaactcggt
    16441 ttgccgattg cgctggcact attctttgaa gtgacactgt ttgccgtcgt ggctctgtta
    16501 gtgtctccgc tcggtattgt tgatgtcgca ggacaccaga ttgccctgaa ctttagttca
    16561 ctgatgtttg tgcttccaat gtcgctggcg gcagcggtaa ctatccgcgt aggttatcgt
    16621 ctgggtcagg gctcaacgct ggatgcgcaa accgctgcgc ggaccgggct tatggtgggt
    16681 gtctgtatgg caaccctgac ggccatattc acggtttcac tgcgggagca aatcgccctg
    16741 ttgtacaacg acaatcccga ggttgtaacg ctggctgcgc atttgatgtt gctggcggcg
    16801 gtatatcaga tttctgactc aatccaggtg attggcagtg ggattttgcg tggttataaa
    16861 gatacgcgtt ccattttcta tatcaccttt acggcttact gggtgctggg cttgccaagc
    16921 ggctatattc tggcactgac cgatctggtc gttgaaccta tggggccagc aggcttctgg
    16981 ataggcttta ttattggcct gacgtcggca gccattatga tgatgctgcg tatgcggttc
    17041 ctgcaacgtc tgccgtcagt tatcattctg caacgagcat cccgctaata aagacaaggc
    17101 gcaaccttca cgggttgcgc ctgtgttttt acgcaggctg gagcgttgcg ccaatcccgt
    17161 cttcgtctgg ctgtaatttc agagcgttgc acagagtttg ccgactggca tctgttatct
    17221 caacgtgtat atccccggta acttcccctt tcacattgat gaagtcaggt gagatttctt
    17281 tcgcactggt ttgcaggcaa atcgctccct ctccgcgcag gcgtttcact ttcagttgca
    17341 catgcgttgc cgcaccagtg atcagccaca gttttccgcc attcagattg atgttaccaa
    17401 caataaaagt gacaccgtca ccttttaccg tactgccgtc gagaatcaac acaccgccct
    17461 gctgtacagt aatcgttgat tgttgcagat ctgcgccata gccaataatc attgaagcgc
    17521 cgtccagtaa tgcaacactc ccggtcagtt caaccggtgc gcgatcttcc tctacgacaa
    17581 aattttcgtc cgcgctggtg ggtgacaaca ggatcactgt tccaccttgt attaccgcat
    17641 tttccagcgt accggcatgc acttcagccc gaccacccac attcacggac aggttttgcg
    17701 tcttaccgga actgtagagc tgaaggccat ccgtccccag acgataaatg gatccattct
    17761 caacaatagt gtcagagtct tcaccaccga gattattcat ggtgccacca gcaagcaacg
    17821 tatcactggc tttcccggcc agcataaaaa cgccgccctg attaatctca caattgctgg
    17881 cttccccacc tggatacacc aacaattcac caccttcatc taccacgacc gctgaggttg
    17941 tcccattaac caacaggccg ccctcttgat cgagaatgat tttttccgcg cgatgtcctt
    18001 ccagtacacg caggttaccg ccattttcca gcaacaaacc gcaggcataa ccctgatcaa
    18061 cgctgaattc accttcggga tggcggccat taaccgtagc gagcgtagag agactaattg
    18121 cgcctccctc ggactgaaca atatctgtgg caatggcacc gtcgctgacc gatagcgtgc
    18181 cattgttacc gtactgtgtg catttggcga tcccaccggt tgccactttc aaccagccag
    18241 cccccgtgac cacggtattt tcggctcttc cgccagcttc aacaatcaac tcgccttcag
    18301 taagttgcgt cgcgccaatg acctcgccgg tcttaacaat ttgcacattc ccgtcgctca
    18361 tcaagttttt cgcatcagat cccatgattt attcctttgc tgcatctgtg tgcctttatt
    18421 gctacctaag tgtaaaggct acggaggatt tatccacgac agatttgaga tggtggcaaa
    18481 caactctgtt taaactctga tacacgaatt attgggttgt atcagatgta aatgcgatcc
    18541 tgaataaaaa tcgcccttgc aaatcaacaa aatatagcca atttgcttaa aagctctgca
    18601 aacggtcgaa atagcgcaga aaattacgtt ttgcctcttg ccaccttccc actctgccgc
    18661 taatattcgt ccccgttgtc acctacaacg ttgcgttcat agctcagttg gttagagcac
    18721 caccttgaca tggtgggggt cgttggttcg agtccaattg aacgcaccat cctgcgtccg
    18781 tagctcagtt ggttagagca ccaccttgac atggtggggg tcggtggttc gagtccactc
    18841 ggacgcacca gattttctta atctggtctt ctcctttttc cctctgtttc ttctctgtat
    18901 ccaatacgtt aaaagattta cactgtcttc gtatgcgtta tcagaaggag aatcgctatg
    18961 gcaactttgt tacaacttca ttttgctttt aatggcccat ttggcgacgc aatggctgag
    19021 cagcttaaac cgcttgctga gtcgattaat caggaacctg gttttctgtg gaaggtatgg
    19081 acagaaagtg aaaagaacca cgaagccggt gggatctatc tttttactga tgaaaaaagc
    19141 gcccttgcct atctggaaaa acatactgtc cgacttaaaa atctcggcgt tgaggaagtt
    19201 gtcgccaaag ttttcgatgt caatgagcca ctgagtcaaa tcaatcaggc aaaactcgcc
    19261 tgacagaatt taatcaaggg cggttagcgc ccttttcatc cctgtctgaa atttctcaaa
    19321 ttctaaaaat ctcaaccaaa cttatctgat aacactaaat tcgaaagaat gcgtacaggt
    19381 aagtaacaat gaaaaaaatt gctattgtgg gtgccgggcc tacggggatc tacaccttat
    19441 tctcgcttct acagcaacaa actccacttt ctatttctat cttcgagcag gctgacgagg
    19501 ccggtgtcgg gatgccatac agtgatgagg aaaactcaaa aatgatgctg gcaaatattg
    19561 ccagtattga aataccgccg attaattgta cgtatctcga atggctacaa aagcaagaag
    19621 ccagccatct ccagcgttat ggcgttaaaa aagaaacctt gcacgatcgt cagtttttac
    19681 cgcgaattct gctgggcgaa tatttccgcg atcaattttt acgattagta gaccaggcac
    19741 gacagcaaaa atttgcagtg gctgtttatg aatcatgcca ggttaccgat ctgcaaatta
    19801 caaatgctgg cgtcatgctc gctacaaatc aggatttacc cagcgagacg tttgatttag
    19861 cggtgattgc cacgggtcac gtctggcctg atgaagaaga agcaaccaga acgtattttc
    19921 ccagtccgtg gtcaggcctg atggaagcaa aggtcgatgc gtgtaacgtg ggtattatgg
    19981 gaacatcctt gagcggactg gatgcggcaa tggcagtggc tattcagcat ggttcgttca
    20041 ttgaagatga taaacaacac gtcgttttta accgcgataa cgcaagtgaa aagctaaata
    20101 ttacgttgat gtcgcgcacg ggtattttac ccgaagccga tttctattgc cctattccct
    20161 acgagccctt acacatcgtc accgatcagg cattaaatgc tgagattcaa aaaggtgaag
    20221 agggcctttt ggatcgggta tttagattga tagtagagga aatcaagttt gctgatccag
    20281 actggagcca acgcatagcc ttagagagcc tgaatgtcga ttcctttgct caagcctggt
    20341 ttgccgagcg caaacaacgc gacccatttg actgggcaga aaaaaatctc caggaagtcg
    20401 aacgcaataa acgagaaaaa catactgttc cctggcgtta tgtcattctg cgcctgcatg
    20461 aagccgtaca ggaaattgtt cctcatctga atgaacacga ccataaacgg ttcagtaaag
    20521 gccttgccag ggttttcatc gataattatg cggcaatccc ttcagagtct attcgtcgcc
    20581 tacttgcctt acgtgaagcg ggaatcattc atattctcgc cctcggtgaa gactacaaaa
    20641 tggaaattaa tgagtcgcgc accgtcctga aaacggaaga caacagctac tcgtttgacg
    20701 tttttattga tgcccgcgga cagcgtccgc ttaaagtgaa agatattcct ttccctgggc
    20761 tacgcgagca attacagaaa acaggggatg aaatccctga tgttggcgaa gattatacgt
    20821 tacagcaacc cgaagatatt cgtggacgcg tagcgttcgg cgcgttgccc tggttgatgc
    20881 acgaccagcc tttcgttcag ggacttacgg catgtgcaga aattggtgag gcgatggctc
    20941 gggcggtcgt aaagcctgca tcccgtgctc gtcggcgtct ttcgtttgat taaaggtaaa
    21001 gctatcagca ctcaacaaac tgatgctgtt cattaagacg atagcggact ccggttcgaa
    21061 tattgttttc accttcaatg gcgacggcga agcgaactct ttcgccgtca tgatatgcca
    21121 gtgcggcgct tccccctggc ccgagaataa ttgagtcgac cacgcccgtg ctcactaccg
    21181 ttgaattttc accgctggca atgatgtgta cattgtcgcc gctgttggca attctggcgt
    21241 ttgcgccaaa gctggcgatt tgcaccaggt caccattact ggcgacatga caccgttcac
    21301 ccaatgtaca aacgcgtact cgcattccgg tattcgcaat ccgactacta tctccagcgc
    21361 tgcttatccg cgaagaatta ccggttacag caacccgtgc gcgttcaccg gagctgccaa
    21421 tgtgactgtt aaagccgaca cttccaatcc gcacgctata acccatactg gcaatttgcg
    21481 cggcataccc agcggttgcg attcgggcgt tatcctcaca acagccgatg cgagcatgat
    21541 cgtcgctgcg ggcaaactga ctgcaaaatg gtatctctcc ggggtctgtg gccagttttt
    21601 gcatagagga aacttcctga tgcgcgaagt tttcatccgc cagccatttt ttccacgcat
    21661 actcaaccag actctccgcc cagtccgtat atccttcctc aatcagtgca tgatgaatat
    21721 cggcataact accgccgcgt gggaaatggc gcaggaacca gcgatacatc accgctccga
    21781 tacgccattc tcgcaggtct gctcgtgtaa tgatcattcg ttagtgccgg tgatagccat
    21841 cgaccatgct tttaaaggtt tcgtgtggcg tacgccccgt ggtgcaaaga taaagatgac
    21901 cgaagataaa aaagaggctt ataaatgcca gagcaaaatg cgcctgcaat aaccagtatc
    21961 ttacgccagg aaacacatct cccacggctt gcggatagag acacagcagc cccgtcaata
    22021 gtaacaacgg cagcaatcca tacataacac caacataggc gacctgctgt aagggattaa
    22081 atttagactg ggttgttgcc gggaaaggat gttcttcccc ctgcataatg ccaaacaaat
    22141 aaaatcgcgt ttgttttgcc gctcgctcca gccacccctg acgacgaatg cgatagtggt
    22201 gaccattacc cccaacggca ttgatcagca caaagccgag ccagcatgcg agtaacaaaa
    22261 atccgcaaac ttcatgcacc gcaaccagac ttttaaccgc agttgcgccc accagcgcaa
    22321 agtgatttat cagcccactt gccagcaaca atacaaagag taacgcattc gaccagtgcc
    22381 ataatctgac cgctttgcag taaagataaa tcttctcacc ataatcggtg actgatttct
    22441 tcacgccacg agcacgaagc aaggcatgca gccccagcac caaccacatc ccaaccagca
    22501 atactccggc aatgatcaac cacaccggcc aaaattcggg agcgaactgt ggcacatagt
    22561 tcgctaactg gctctgaaac tgttcagcat gttgcgacgg gttcatacat tttccttttt
    22621 aatcaaatgt tgaccgaacc ggcgatacag atgcggtttc ccggcaccag gtagctgata
    22681 ttgatagtat ttattttgct gcaaccacgc ctgaatttct gggctatctt cgcgaccaaa
    22741 gatcaatgcg tgttccgggc aggcgctgac gcaaatgggc ggaaagcctt ttgccagtcg
    22801 ggactcagcg cagaaatcac atttatctgc cactttagtg acagggttaa ggtagcgaac
    22861 ctggtacgga caagcgccaa tgcaataact gcagccgatg cactgcgatt tttctacccg
    22921 cacgatcccc tgctcatcac gccacgacgc ccctgtcggg catacgtcga tgcacggagc
    22981 atcttcgcag tgctgacatg actggcgaaa aaagtgatac tgcgtttcgt tgtcgttatc
    23041 ggtgactgga atgtgtgcta tcgataaacg gcttccctga gcggggacgt ggttagtttt
    23101 acggcatgcg cgggcgcaaa tattacagcc attgcaccgt gactcgtcat gaatcatggc
    23161 ataccgaact tccttttctt gcctcgtgtt cgctaacaga gaagacgcag aaccagtaaa
    23221 aaagattact gttcccatcc ccagaacaaa tttgcgtcga gtgaaggaca tctgattatc
    23281 ccctaatgca gtggctgcaa ctgtccaccc tatccatgac atctccctga aatttcaggg
    23341 gttctgaaac tgctctacct cgaaccattc gcataagcca taagccttgc cataggtcat
    23401 aaatcgctcg ctaaacaagt acaacaccgc cgtcgatgac tccacctgcc gtatatcggc
    23461 atattcaggt gcaacatcca tcgcggcgat cgcggcttca atctgcgctt cctggaagta
    23521 atatggcgct tgcatcagca tggcgacttt gtaaggacga ggatatgtct ggcactcaaa
    23581 acgaacggcg tgtgcaatgg cgcgacaaat gtcctgctcc acaacttgca aagacatcgc
    23641 cgcgtagttt tcactcatgg cctgcgttga gtaatagtaa tcatcctggc ttcccgtcat
    23701 acaagcgata tccgcgtagt ctccgttctg cttaatgctt tccagcaaaa ctgacagctc
    23761 ctcttctgca accgaatagg gtggcgagag aaacacggcg cgcgccacca gctgcccagc
    23821 cgcagaatgc tggcgaatga actctgccag gatttcagcc ggttctggtt cggttaccgt
    23881 atcaacgggg tgacgcatcc cctgtaacca ttgccgcttc gcctcgtcaa cttcgctgac
    23941 tttcgctaac ggcaattcat cccgatggtt catctcaccc attccttaca caggcagtag
    24001 attgtgtgct gccagatcgg cggcaatatc ttccagcccc agacgctgca atgtttcgcg
    24061 ggtcggacaa ccaagctgtg ggtcccagcc catttctttg tagaacatcg tgagcgaagc
    24121 atgcatatcg tcacgatcca ttttgtcagt accttcagta aagaccggga tctgcggatc
    24181 cttgtcgaat acccaggaac agataagatc gtgttcatta cgcatatctt tggtttgcat
    24241 cagttttacc gtgtaggcac gatgcaacgt aaagatacgc tctgcagcta aatctaattt
    24301 ttcctgagtc atctcttcgc cggtgatcgc tttgaagaat ttggcttcaa gcgccagatc
    24361 gccccggtaa ttacggcttt tcagcggcga aacggtcatt ggccagaccc agttgcacag
    24421 ggtgacggcg ttatgcaaac agacccgcaa cagcgaccac tttgcatatt taatttttgc
    24481 gtcattgatt ggcgtgtagt ttttggtttc atcgtaggca tcttcagaac caaaaagctc
    24541 tttcgccact tcacgctgca gtttcaatgg caaacccgaa ccaataaaat tgatatgggt
    24601 atgcgtcatg cagtcacggt tgaacataca gttaacaatg gaaccgacct gcgctgacgc
    24661 ttcattggca tggtgaaccg gatagccaaa cggcgaccag agtttatttt tcgcgtagcc
    24721 ccagtactct tcacccaaat tccagcgttc tgcgatggca tatgaaccat cagccaggtg
    24781 actcagctca cccacacgat gcgccagacg gtagtaaaaa tctttaatga agttaacgtc
    24841 acccgcttcc agttgatccc agcgaatttc cgcatactct tcagcaggca gaacacgctt
    24901 gaacacccct ttgctgtaac aataggtaaa gtcgcgatgc aactgcccgt agttacacca
    24961 taggccgtag tcatcgaaca aattcagacc caccaggtta ccaatcacac ggccatcgtc
    25021 tttatcttca aaatctttcg gaccgttcgg gaagatggtg gtatggacaa agttggctac
    25081 acaagtgtta ccacctgtgc tgggcacgcc aaactctttc acccgaggaa tattcatttg
    25141 ggtcatacaa cggatcgggc aagagtggca gccactcatt tttactgtgt atttctctgc
    25201 cgccggacct aagtcaaaaa cggatttata ggtacgaaag ccgaccgtgt tctgattgcc
    25261 tggcggaatt tcccccgttt caatcggacc accttcagcc gcgccccaaa acagcccttt
    25321 acgtgctgtc caacgtgact tgggatctga atactctgcc cacgattgcg gcgtacttgg
    25381 cacgacatgg ttgttattcg caccaataag ttcagtcatc atgtaatcgt tgagacgctt
    25441 catctcctga cgatcggcaa tgttgacccc tttcgtccct tcaaccgcaa tcgctttcag
    25501 gtttttcgaa cccataattg cgccagttcc cgcaccgccg ctgtggttac cgctattcaa
    25561 catgccagag agaggaacaa ggttttcccc agcctgacca atagccgcca cacaggtttc
    25621 cggactggtc aatcgacaaa tttcttccgt cgttgcgcgc gtcccttttc cccataagaa
    25681 atcggctttt tccaggctaa ctttgtcatc tttaatcttc agccataccg gtgatttcgc
    25741 cttcccttcg ataataatga cgtcgtatcc agcgaatttc atttgcgctg caaaaaagcc
    25801 acccatatgg gcatcgacaa ctaaatttcc tttggtaaaa gtagaaagtg aggtgatatt
    25861 tacgcgagaa ctacaggggg caccagatcc agttaatggg ccggtagcaa agactaattt
    25921 attcgcttca tcgaaaggtt tcgtgcctgg cggtacttcg tcatacataa ttttgtagcc
    25981 gaagcccatg ccaccgacaa aacttttaaa cttactggaa tcttcgaggg taatatttcc
    26041 tgtcgtgaga ttgactctta atatattacc tgtccaaccg ttagccatga ttttttcctt
    26101 tgcaagattc acacagtaat atctttccac tcgataattt ttaacgcccc tgttgggcag
    26161 gcgtttgcgc attcaccgca taacacacat ttcgaggatt ttttactttc ggtatttacg
    26221 gtggccatca tccacgggca tgccgtagta caggcgctac agccaataca gcgtttatga
    26281 tcgacggtaa tacagccttc tttctgctgc caggtaatcg caccaatcgg gcagacgttc
    26341 atgcattgcg gctctttgca ttgacggcag gtgtccgcgg tatagttgag atcgccatac
    26401 aggccgccgc cagagccaac cccgttgtcg ccaaagaaat aattgcggtg gattttgata
    26461 cgggagaaga atgtccctac tgagccatcg ttgaagttgg tacaagagat ttcacagcgg
    26521 tggcaacccg tgcatcgcgc tcgctgggtc accagcaccc ctttgggtgt atttatcaac
    26581 cctactgtgc cgctatcaat atcttcctgt ttgcaaccca aaagtgacag taacgcaggg
    26641 gcaattgtta gaccagcaag gccttttcct gatatacgca agaattctaa tcgcgttaag
    26701 ccaatatcta atagtggacg atcaaccggg ttcatttatt tcgcatcctc ttcgcaaaca
    26761 cagtgctgac aattcagcag cctgatatta agcattcaac taatacgctg ctgactctgt
    26821 ccccaacacc agatattttc cctaacccga tagtgtatga gaaaagattt aacgccgata
    26881 aactaatcct ttatggttag gagaaaaaat aatgtgatta ttctgagccc ttaacattga
    26941 t
//
LOCUS       DAZGAN020000060        26739 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGAN020000060 DAZGAN020000000
VERSION     DAZGAN020000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.060"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            76..1674
                     /gene="rtcR"
                     /locus_tag="ACHKYO_RS13950"
                     /old_locus_tag="pgaptmp_002790"
     CDS             76..1674
                     /gene="rtcR"
                     /locus_tag="ACHKYO_RS13950"
                     /old_locus_tag="pgaptmp_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HGU4429608.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1656..2414)
                     /locus_tag="ACHKYO_RS13955"
                     /old_locus_tag="pgaptmp_002791"
     CDS             complement(1656..2414)
                     /locus_tag="ACHKYO_RS13955"
                     /old_locus_tag="pgaptmp_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HGU4429609.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2431..3261)
                     /gene="glpG"
                     /locus_tag="ACHKYO_RS13960"
                     /old_locus_tag="pgaptmp_002792"
     CDS             complement(2431..3261)
                     /gene="glpG"
                     /locus_tag="ACHKYO_RS13960"
                     /old_locus_tag="pgaptmp_002792"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HGU4429610.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3306..3632)
                     /gene="glpE"
                     /locus_tag="ACHKYO_RS13965"
                     /old_locus_tag="pgaptmp_002793"
     CDS             complement(3306..3632)
                     /gene="glpE"
                     /locus_tag="ACHKYO_RS13965"
                     /old_locus_tag="pgaptmp_002793"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HGU4429611.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3822..5327
                     /gene="glpD"
                     /locus_tag="ACHKYO_RS13970"
                     /old_locus_tag="pgaptmp_002794"
     CDS             3822..5327
                     /gene="glpD"
                     /locus_tag="ACHKYO_RS13970"
                     /old_locus_tag="pgaptmp_002794"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HGU4429612.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5381..5986)
                     /locus_tag="ACHKYO_RS13975"
                     /old_locus_tag="pgaptmp_002795"
     CDS             complement(5381..5986)
                     /locus_tag="ACHKYO_RS13975"
                     /old_locus_tag="pgaptmp_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429613.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5989..6756)
                     /locus_tag="ACHKYO_RS13980"
                     /old_locus_tag="pgaptmp_002796"
     CDS             complement(5989..6756)
                     /locus_tag="ACHKYO_RS13980"
                     /old_locus_tag="pgaptmp_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429614.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(6759..7469)
                     /locus_tag="ACHKYO_RS13985"
                     /old_locus_tag="pgaptmp_002797"
     CDS             complement(6759..7469)
                     /locus_tag="ACHKYO_RS13985"
                     /old_locus_tag="pgaptmp_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429615.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7485..8993)
                     /locus_tag="ACHKYO_RS13990"
                     /old_locus_tag="pgaptmp_002798"
     CDS             complement(7485..8993)
                     /locus_tag="ACHKYO_RS13990"
                     /old_locus_tag="pgaptmp_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU4429616.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNVDNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNDDKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(9118..11565)
                     /gene="glgP"
                     /locus_tag="ACHKYO_RS13995"
                     /old_locus_tag="pgaptmp_002799"
     CDS             complement(9118..11565)
                     /gene="glgP"
                     /locus_tag="ACHKYO_RS13995"
                     /old_locus_tag="pgaptmp_002799"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HGU4429617.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11584..13017)
                     /gene="glgA"
                     /locus_tag="ACHKYO_RS14000"
                     /old_locus_tag="pgaptmp_002800"
     CDS             complement(11584..13017)
                     /gene="glgA"
                     /locus_tag="ACHKYO_RS14000"
                     /old_locus_tag="pgaptmp_002800"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGU4429618.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(13017..14312)
                     /gene="glgC"
                     /locus_tag="ACHKYO_RS14005"
                     /old_locus_tag="pgaptmp_002801"
     CDS             complement(13017..14312)
                     /gene="glgC"
                     /locus_tag="ACHKYO_RS14005"
                     /old_locus_tag="pgaptmp_002801"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGU4429619.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14330..16303)
                     /gene="glgX"
                     /locus_tag="ACHKYO_RS14010"
                     /old_locus_tag="pgaptmp_002802"
     CDS             complement(14330..16303)
                     /gene="glgX"
                     /locus_tag="ACHKYO_RS14010"
                     /old_locus_tag="pgaptmp_002802"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HGU4429620.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16300..18486)
                     /gene="glgB"
                     /locus_tag="ACHKYO_RS14015"
                     /old_locus_tag="pgaptmp_002803"
     CDS             complement(16300..18486)
                     /gene="glgB"
                     /locus_tag="ACHKYO_RS14015"
                     /old_locus_tag="pgaptmp_002803"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HGU4429621.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18759..19862)
                     /gene="asd"
                     /locus_tag="ACHKYO_RS14020"
                     /old_locus_tag="pgaptmp_002804"
     CDS             complement(18759..19862)
                     /gene="asd"
                     /locus_tag="ACHKYO_RS14020"
                     /old_locus_tag="pgaptmp_002804"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU4429622.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            20055..20648
                     /gene="yhgN"
                     /locus_tag="ACHKYO_RS14025"
                     /old_locus_tag="pgaptmp_002805"
     CDS             20055..20648
                     /gene="yhgN"
                     /locus_tag="ACHKYO_RS14025"
                     /old_locus_tag="pgaptmp_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HGU4429623.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20705..22045)
                     /gene="gntU"
                     /locus_tag="ACHKYO_RS14030"
                     /old_locus_tag="pgaptmp_002806"
     CDS             complement(20705..22045)
                     /gene="gntU"
                     /locus_tag="ACHKYO_RS14030"
                     /old_locus_tag="pgaptmp_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU4429624.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(22049..22576)
                     /gene="gntK"
                     /locus_tag="ACHKYO_RS14035"
                     /old_locus_tag="pgaptmp_002807"
     CDS             complement(22049..22576)
                     /gene="gntK"
                     /locus_tag="ACHKYO_RS14035"
                     /old_locus_tag="pgaptmp_002807"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HGU4429625.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLMVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(22715..23710)
                     /gene="gntR"
                     /locus_tag="ACHKYO_RS14040"
                     /old_locus_tag="pgaptmp_002808"
     CDS             complement(22715..23710)
                     /gene="gntR"
                     /locus_tag="ACHKYO_RS14040"
                     /old_locus_tag="pgaptmp_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HGU4429626.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDADLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(23934..24629)
                     /gene="yhhW"
                     /locus_tag="ACHKYO_RS14045"
                     /old_locus_tag="pgaptmp_002809"
     CDS             complement(23934..24629)
                     /gene="yhhW"
                     /locus_tag="ACHKYO_RS14045"
                     /old_locus_tag="pgaptmp_002809"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HGU4429627.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(24752..25789)
                     /gene="yhhX"
                     /locus_tag="ACHKYO_RS14050"
                     /old_locus_tag="pgaptmp_002810"
     CDS             complement(24752..25789)
                     /gene="yhhX"
                     /locus_tag="ACHKYO_RS14050"
                     /old_locus_tag="pgaptmp_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU4429628.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            26122..26610
                     /gene="yhhY"
                     /locus_tag="ACHKYO_RS14055"
                     /old_locus_tag="pgaptmp_002811"
     CDS             26122..26610
                     /gene="yhhY"
                     /locus_tag="ACHKYO_RS14055"
                     /old_locus_tag="pgaptmp_002811"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU4429629.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         6669 a         7148 c         6663 g         6259 t
ORIGIN      
        1 acgtccgtaa acggagattt tccacaaaac caattcaccg ataatgaaat atcgtctttt
       61 ataaggatat ctaagatgcg taaaacagtg gcttttggct ttgtcggtac cgtgctggat
      121 tatgccggac gcggcagtca gcgctggtcg aaatggcgtc cgacgctttg tttatgccag
      181 caagaatcgt tagtcatcga tcggctggaa ttgttgcacg acacccgctc gcgctcgcta
      241 ttcgaaacgc ttaaacgcga tatcgccagc gtttcgccag aaacagaagt cgtgggcgtt
      301 gagattgaac tgcataaccc gtgggatttc gaagaggttt acgcctgcct gcatgatttc
      361 gcccgtggtt acgagtttca gccagaaaaa gaagactatt taattcacat caccaccggc
      421 acccacgtcg cgcagatttg ctggtttctg ctggcggaag cgcgttacct gcccgcccgg
      481 ctgatacaat cttcacctcc gcgcaaaaaa gaacagccgc gcggcgcagg tgaagtgacg
      541 attatcgatc tcgatttaag ccgttataac gccatcgcca gccgctttgc cgaggaacgc
      601 cagcaaacgc ttgattttct taagtccggc attgccacgc gtaaccccca cttcaaccgc
      661 atgattgagc agatcgaaaa agtggcgatc aaatcccgcg cgccgattct gcttaacggt
      721 ccaaccggcg cgggcaagtc atttctggcg cgacgcatct tcgagttaaa acaggcgcgg
      781 catcagttta gcggcgcgtt tgtggaagtg aactgcgcca ccctgcgcgg cgataccgcc
      841 atgtcgacgc tgtttggtca tgtaaaaggc gcgtttaccg gggcgcggga atctcgtgaa
      901 ggtttattac gcagcgccaa cggcggaatg ttgtttcttg atgagattgg cgaactgggc
      961 gcagacgaac aagcaatgct gctaaaagcc attgaagaga aaacctttta cccgtttggc
     1021 agcgatcgcc aggtgagcag tgattttcag cttatcgccg gaacggtgcg cgatttgcgc
     1081 cagctggttg ccgaaggcaa atttcgcgaa gatctgtacg cgcggatcaa tctctggacc
     1141 ttcaccctgc cgggtctacg ccagcgccag gaagatattg aaccgaacct tgattatgaa
     1201 gtggagcgcc acgccacact cactggcgac agcgtgcgtt ttaacaccga agcgcggcgc
     1261 gcctggttgg cttttgcgac ctctccccag gcaacatggc gcggtaactt tcgcgaactt
     1321 tctgccagcg tcacgcggat ggccaccttt gccactagcg gacgcatcac tctggacgtg
     1381 gttgaagatg agataaaccg tctgcgctat aactggcagg agagccgccc ctccgcgctt
     1441 acggcgttgc tgggcgcaga ggcagaaaac atcgaccttt ttgacaggct gcaactggaa
     1501 cacgtgatag ctctctgtcg ccaggcaaag tcgctttccg ctgccggacg cctgcttttt
     1561 gatgtttcgc gccagggcaa agccagcgtc aatgacgcgg atcggctacg caaatacctg
     1621 gcgcgttttg gtctgacgtg ggaagccgtg caggatcagc acagctccag ttgaatatga
     1681 tggtccgtca gcacctgcat tacgcttact ggcggcgggg cgtcggtgta gacggcatct
     1741 accatgctga tgctgcccat attgaccatc gcgttacggc caaatttcga gtgatcgaca
     1801 accagcataa cgtggcgcga gttctcaata atggcgcgct tggtgcgaac ttcgtggtaa
     1861 tcgaactcca gcagcgagcc gtcgctatcg atgccgctta tccccagaat gccgaaatca
     1921 aggcggaact gggagataaa atcgagcgtc gcttcgccaa tgatcccgcc atcgcggctg
     1981 cgtaattcgc caccggcgag aataatgcgg aaatcttctt ttaccatcaa cgtgttagca
     2041 acattgagat tgttggtgac aatacgcaaa ttgctgtgat taagcagcgc gtgcgccacc
     2101 gcttccggcg tggtgccgat atcgataaac agtgtcgagc cgttggggat ttgctccgcc
     2161 actttgcggg caatcctctc tttttcttcg gtctgggtgg ctttgcgatc gtgccacggc
     2221 gtgttaaccg aactggaagg cagcgccgca ccgccatggt gacgcaggat caggttttgc
     2281 tccgccagct cattgaggtc gcggcgaata gtctgcgggc tgacggagaa atgctctacc
     2341 agctcttcag tactgacata accctgctgt ttaaccagtt cgataatacc gttgtgacgt
     2401 tgtgtttgtt tcatttataa atccctggaa ttattttcgt tttcgcgcat tgagcgaatc
     2461 aacaaaagcc atcgctaaac ccacggctaa cccggcgatg tgtgctccgt tcgccatcga
     2521 catcccaaac aaatcaaacc atccggcgac aatccagatc agcgcaaaga taattaaccc
     2581 acgttgcagg taaatgccac tttgcggatc gcgttcacca cgtagccaga cgtagcccat
     2641 cagcgcatac accacgccag aaagcccgcc aaaccacggc ccgctgaatt tttgctgcac
     2701 atagccgctt aacagggcgc taatgagagt aataacaatt agcttaccgc taccgaggcg
     2761 tttttccacc gcaccgccga gataccacca ccagagcagg ttaaagagga tatgcatcag
     2821 cgagaagtgc attaacgcgt gggtgaagta acgccagaac tcaaatttca gtgttggatc
     2881 gaatggccag gccagccata acatcacttc ctgatcgccg agaatttgca tggcgataaa
     2941 caccaccacg caggcgatca tcaccaccca ggttaccgga cctgcgcgtt cacgcaaggc
     3001 ggcaaagaaa ggataacggc gataatgcag gccactgccg gtatggcctg cctgccagct
     3061 ggccgccaga taacgcggat ctgccgggtt ttcgagaaaa cgcgccagct ccgcccgtac
     3121 gcgctcggcc tgggactcat ccgccagcca gacatcgctt tggttatgtt gttgaatcgt
     3181 gaggataaca ccctgcgtcg ccatgtaatc aacaaacgcc tgcgccacgc gggggttagc
     3241 aaaagaggta atcatcaaca tcgttgctgt cgcttattcc acacaaaagg ggacagtata
     3301 aagcgttacg cgccgtacgc cacctctgcg ggaaactgac gttgccaggc ttcaaagccg
     3361 ccgtcaatgc tatagaccac atcgtagccc tgttgcagca gatactgcgc cgcgcctttg
     3421 ctgctattgc cgtgataaca catcaccatc accggagtgt caaagtcgtt atcacgcata
     3481 aacgcgccca gcgtgtcgtt ggttaaatgg aaagcctgca ccgcatgccc catagcgaaa
     3541 ctctgtggat cgcgaatatc gaccagcacc gcctcttttt cctgcaactt ctggtgcgcg
     3601 tcggcaacgt taatacattc gaactgatcc atgcgtctct ctttctttac aaacaagtgg
     3661 gcaaatttac cgcacagttt acgtcgaagc ggcagataaa cgccataatg ttatacatat
     3721 cactctaaaa tgttttttca atgttaccta aagcgcgatt ctttgctaat atgttcgata
     3781 acgaacattt atgagcttta acgaaagtga atgagggcag catggaaacc aaagatctga
     3841 ttgtgatagg tggcggcatc aatggtgctg gtatcgcggc agacgccgct ggacgcggtt
     3901 tatccgtgct gatgctggag gcgcaggatc tcgcttgcgc gacctcttcc gccagttcaa
     3961 aactcattca cggtggcctg cgctaccttg agcactatga attccgcctg gtcagcgagg
     4021 cgctggctga acgtgaagtg ctgctgaaaa tggctccgca tatcgccttc ccgatgcgtt
     4081 ttcgcctgcc acatcgtccg catctgcgcc cggcgtggat gattcggatt ggtctgttta
     4141 tgtacgatca tctgggtaaa cgcaccagcc tgccgggatc aaccggtttg cgttttggcg
     4201 caaactcagt gttaaaaccg gaaattaagc gcggattcga atattctgac tgttgggtag
     4261 acgacgcccg tctggtactc gccaacgccc agatggtggt gcgtaaaggc ggcgaagtgc
     4321 ttactcggac tcgcgccacc tctgctcgcc gcgaaaacgg cctgtggatt gtggaagcgg
     4381 aagatatcga taccggcaaa aaatatagct ggcaagcgcg cggcctggtt aacgccaccg
     4441 gcccgtgggt gaaacagttc ttcgacgagg ggatgcatct gccttcgcct tatggcattc
     4501 gcctgatcaa aggcagccat attgtggtgc cgcgtgtgca tacccagaag caggcctaca
     4561 ttctgcaaaa cgaagataaa cgtattgtgt tcgtgatccc gtggatggac gaattttcca
     4621 tcatcggcac caccgacgta gagtataaag gcgatccgaa agcggtgaag attgaagaga
     4681 gtgaaatcaa ctatctgctg aaagtgtata acgcgcactt taaaaaacag cttagccgtg
     4741 acgatatcgt ctggacctac tccggtgtgc gtccgctgtg tgatgatgag tccgactcgc
     4801 cgcaggctat tacccgtgat tacacccttg atatccatga tgaaaacggc aaagcaccgc
     4861 tgctgtcggt attcggcggt aagttgacca cctaccgcaa actggcggaa catgcgctgg
     4921 aaaaactgac gccgtattat cagggcattg gcccggcgtg gacgaaagag agcgtgctgc
     4981 cgggtggtgc cattgaaggc gaccgcgacg attatgccgc tcgcctgcgc cgccgctatc
     5041 cgttcctgac tgaatcgctg gcgcgtcatt acgctcgcac ttacggcagc aacagcgagc
     5101 tgctgctcgg caatgcggga acggtaagcg atctcgggga agatttcggt catgagttct
     5161 acgaagcgga gctgaaatac ctggtggatc acgaatgggt ccgccgcgcc gacgacgccc
     5221 tgtggcgtcg cacaaaacaa ggcatgtggc taaatgcgga tcaacaatct cgtgtgagtc
     5281 agtggctggt ggagtatacg cagcagaagt tgtcgctggc gtcgtaagga tgatgcccgg
     5341 ttgtgaagtg caaccgggct atttggattc cttagactat tcattgtaaa taattgaata
     5401 acggcccctg agctaaaatc gccaaactta agcacgttac ccaatcgccc cacttcttcc
     5461 tctggtttct attttttctg caccaatggc aataatatcc caatgcaaaa ccaagctgtg
     5521 cactaatcgg gccgacaaaa tcagtccaag gctgcggcca aaaaggggaa aaattcaggg
     5581 gaagccagac aaacgtcaga aagaaatttc ggatattttc cagcaacgct gatttctctg
     5641 gagaaatata aagaatgaaa ttcgagagaa ttaaccagaa atagaccggc gaaaagacca
     5701 gccagcaatg cagtctgttt tttaattcag gaggcgttct gcgggagaat tgccaaccga
     5761 tgccagccat tacactgaaa aaaagtgcat attcaaccat taactcaagt tgtagttcct
     5821 gaagagaaat acgcacaacc aggagcggaa aggagaatat tatttctgcc agaaaagtta
     5881 acgtgaatat aagtacggct aagacaataa ccgtcggcca taccaataat ggcacgataa
     5941 caaaaccgag aattacaggg ggaatataaa gccggaattt aatcattttt attttcctgg
     6001 tgattcggat gatgcgtcat acatcgcgca taaacactca tcaattatcc agtcgtctac
     6061 ccagacctga tgctgtgtgc cctgttctgg ctgctttggt gggttctcaa tcgcttgccg
     6121 cgctctttct ttcagtaaag gatgattatt tctagcccag gcattgattt gggtggtaat
     6181 ggcgttatcg atatcgtcct ggttatctct gatataatcc tcacaatatc tgttcaataa
     6241 acaggcaaga gaaacggtcg accaaaagcc gggaactgaa ttaatggcat ccagcaatgg
     6301 cgcaagagcg atatcggcac ggaaaaacat ttccagcata tactgtaatt cgacagcttc
     6361 atcgccggat acccaatcag tcatatactg gcaggcaaat cgctgtaaaa ttgcccgttc
     6421 atccgcacgc cagttttctt tgttggcaag gttgatacgg cgcagagaaa aaactacgtg
     6481 aatattgtca aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa aatagcggac
     6541 ctcatcgcta ttttgcggat caggccctgg gctactattc caggcattga ttaaggaata
     6601 cgggatcgct cgtaacgatg tactccgtaa tgccttttgt tcctgctgac tcaggcaata
     6661 ttcacaacaa accacaaatt tctgcggcac ccgataacga cgaaaggtgt gataaatctg
     6721 ctcaattaat gcctgcatgg cggggctggg ttgcatagtt accccctcgc ccgctgatcg
     6781 cgtgtaaacg gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat aagggcgcta
     6841 cgcgaaaggg aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct ttcctctgcg
     6901 caattttcca ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt atcgcgaaat
     6961 ccgcgtaaac tgttattgtt atgaaatttc caggcatctt taaagacctg aaagatatag
     7021 acatcctcaa tccaggagtc acatttttta agcgggcgct ttgctattat cacccgctca
     7081 tccggattta gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt caggtcacat
     7141 tgaacatctg gtacatcact gatatagggg tcaaatccaa aatcagcacc acaggcacaa
     7201 agacggctaa tggtggcggt atcgagggaa aatcccggcc ataccttgct ggaggttaag
     7261 ccaatattta atgtgacatc atatcgaagc aatccctcaa gaccgtcagc accgacgaac
     7321 ttttcccctt catccagggc ctggttaata agttgctgaa caatgccttc atcccagtat
     7381 gccgatttgc agttggcaat cgtaatatcc acgcctgtga agggatcagt accggcaagg
     7441 cttgcaaata atgtattaac taattccatt tttattatcc agctttaagg tagcgtctta
     7501 tacatcggga cataacctac atcttccacc aaactctgtc cctgcggcgt taaaaaccac
     7561 tccaacaatt tttgtgtctc tgacgtcgta ttttcccgcg ttaccataaa ggcatcgacg
     7621 atatagggat atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc gttaatcgct
     7681 agcagtttta tatttttatc gtcgttcatt tgtgttgcgt aataacggaa ggtgtaacct
     7741 attgcgttgt ttgtattacg gtattcggca acgactttaa tcattccctc catcatactt
     7801 gccacttctg tttcctgcgg cgagatcatg cggacatttt tcatgacctg ggattgcatc
     7861 accgtctggc taccagagtc ttccgggcgt tgccaggtct ggatctcctg atcgttaccg
     7921 ccaaccgtgc gccagttggt aattgcacca ctgaagatgt cacgcacttg ttgttcggtc
     7981 agggaattaa ccgggttatc tacattgacg atgaaaacaa acgcttcacg ggcaaaaggc
     8041 gtgtaaatca aagtgacgcc cgattcctcc gcgcgttttt tctgcccacc ggaaggttga
     8101 gccacaaaga taatatcggc attaccttta acaatcttgt tatatgcttc gggagtacga
     8161 gagttcgcca gatattccca ttcgtgaaaa tcttccggca aaacgcttag tgcataaaag
     8221 gcagaggcat aaatggggta cgccgccgtt gcgccatcaa gtcgcggcca gttttgcgtg
     8281 aactgaattt gcggtttacc acgcagtggt gtcagttgat tattgagttt atcgggtcgc
     8341 caggcccagg tatcaatatt ttcacgcaca ccgacgccgg gattaagctg tttatataaa
     8401 taagcctggt aaccggcgac aaaaccaagc atgagaagga taaacaagag agaattacgg
     8461 caacgtaacc cggacgtgcc cgttaaagga tatctggtcg gccagccgta ccccagcgaa
     8521 aaactgatat gcgcgccaac aggtataatg actaaccacc agtactcacc accgactaat
     8581 gaggctaaca aaacgcccag ccaggggagc ataagatcag ccaaccaacc cgctcccatc
     8641 tggcgaaatt gataatctgc cagatgcagc gcgattatcc agacccccag ggttaataat
     8701 gcgggtacta aaaaaagaca ttgtcgccag acggctattt ctcttttttc tggtgcacta
     8761 tcgtccgccc acactcgccc cgagaaaaat agcattaatg cgcccagcgc cagcataaaa
     8821 atgttgagca gaaggtaaat tgttggcgtg ataaagatat tacaaacttc aataataccg
     8881 gcaagtccga cacccagcat ggcaataacc gccgccaaaa actgcgccag aatggggatg
     8941 ccgaaaaaag tcattaccag cgaggtcaaa atccattttc tgttttgcat tattctttcc
     9001 attctttctg aatggcgaaa ttatactccc cgagtcccct tgccccttct ggacactttt
     9061 ccgaaatgat ggcgaaaaaa acgggaccct ttggccccgt tctatttatt agtgaaatta
     9121 caatctcacc ggatcaatat gccagatatg atcggcgtac tctttgatag tacggtcaga
     9181 agagaagtag cccatattgg caatgttcag catcgctttt gcggtccact cttcctgaag
     9241 ctcgtagagt tcgtcgactt tatcctgaca atcgacatag ctgcgataat ccgccagaac
     9301 ctgatagtga tcgccgaagt tgatcagcga atcaaccaga tcgcgatagc gacccggatc
     9361 ttccggactg aatacaccgc tgccgatttg cgtcagcacc tgatgcagct cctcatcttt
     9421 ctcgtagtat tcacgcggtt tgtagccctg acgacgcagt tcttccactt cttccgcagt
     9481 gttaccaaag ataaagatat tgtcagcacc gacatgatcc agcatctcga cattcgcacc
     9541 gtccaacgta ccgatagtaa gcgcaccgtt aagcgcaaac ttcatgttac tggtgccgga
     9601 agcttccgtc cccgccagcg aaatctgttc agacagatcg gccgccggaa tgatcaactg
     9661 cgccaggcta acgctgtagt tcgggatgaa cacgactttc agcttgtcgc caatctgcgg
     9721 atcgttgttg atcactttcg ctacgtcatt gatcaaatga ataatgtgct tcgccatgta
     9781 ataggccgaa gccgccttac cgccaaaaat attcacgcgc ggtacccact tcgcatccgg
     9841 gtcggccttg atgcggttat agcgggtaat cacatgcaac acattcatca attgacgttt
     9901 gtattcgtga atacgtttga tttgtacatc gaacaacgcc tttggattca ccaccacatt
     9961 cagctgctgg gcgatatact ctgccagacg ctttttgttc tccagcttcg cctgatgcac
    10021 agcgtgatta accattggga aatcacagtg ttgttgcagc tcattaagca ggctaaggtc
    10081 ggtgcgccag ttacggccca ggtgttcgtc cagcacggct gaaagcgatg ggttcgctac
    10141 cgctagccag cgacgcggcg tcacaccgtt ggtgacgttg gtgaaacgac ccgggaagat
    10201 tttcgcaaag tcggcaaaca acgattgcac catcagatta gagtgcagtt ccgatacacc
    10261 gttaactttg tggctcacaa caaccgccag ccaggccata cgcacacgac gaccgttgga
    10321 ttcatcaatg atcgacgccc gtcccagcag atcggtatcg ttcggatact gttcctgcaa
    10381 ggttttcagg aaatagtcgt tgatttcaaa gatgatctgc aggtgacgcg gcagaatttt
    10441 acccagcata tcaaccggcc aggtttccag cgcctcgctc atcagcgtgt ggttagtgta
    10501 ggagaagacc tgacaacaca cctcaaacgc gtcgtcccag ctaaattggt gctcatcgat
    10561 cagcagacgc atcatctcag gaatcgacag taccggatgg gtatcattga gatgaatcgc
    10621 gattttatcc gctaggttat cgtaggtttt atgcaactga taatggcggc ttaaaatgtc
    10681 ctgaatggtc gaggaaacca ggaagtattc ctgacgcagg cgcagctcac gcccggagta
    10741 ggtggagtca tccggataca gtacgcgaga tacgttctcg gagtggtttt tatcttccac
    10801 tgccgcgaag tagtcaccct ggttgaattt accgaggtta atttcgctac tggcttgcgc
    10861 actccacaaa cgcaacgtgt tggtcgcgtc ggtgtcgtaa ccagggatta tctgatcgta
    10921 agcgactccc agaatctctt cggtttcaat ccaacgcgtt tttttacctt cctgctgaat
    10981 gcgaccacca aaacggactt tatagcgcgt gttgtggcgt ttgaattccc acgggttacc
    11041 gtattccagc cagtagtctg gcgactcttt ctggctaccg ttaacgatgt tctgtttgaa
    11101 cataccgtag tcatagcgga tgccgtaacc gcgccccggc aaccctaacg tcgccagaga
    11161 atcaaggaag caagccgcca gacgtcccag gccaccgtta ccgaggcctg ggtcattttc
    11221 ttcatcaatc agctcttcga gatttaaccc catcgcttcc agtgcgccct gtacatcttc
    11281 gtaaattcct agcgacaaca tggcgttgga gagcgtacgg ccaatcaaaa actccatcga
    11341 caggtagtaa acctgacgag tttcttgcga caactgggca cggtttgaac gtaaccagcg
    11401 ctccacgaga cgatcgcgca cagcaaataa cgttgcgttc agccattcat gtttattggc
    11461 gacgaccggg tcctttccaa tcgtaaacat cagcttgtaa gcgatagagt gcttaagagc
    11521 ttctacgcta agcgtgggcg atgaatatgt aaacggagca ttcatatagg cgtttcctga
    11581 aaactatttc aagcgatagt aaagctcacg gtacgacttc gccgcgacct gccagctaaa
    11641 atccattgcc atagcctgac gttgcacaaa ccgccacagt gaaggacggg accacagtac
    11701 aaaagcacgt cgaatagccc gtaacagcga ccaggcatta ctatcttcaa agacaaaccc
    11761 actggcgacg ccatctgcaa ggttctcaag agaacagtca gaaaccgtat cagcaagccc
    11821 accggtgcgc cgcactaacg gcagcgtacc gtacttcaat ccataaagtt gcgttaagcc
    11881 gcacggttca aaacggctgg gcaccagaat gacgtccgcg ccgcccataa tgcgatgcga
    11941 aaatgcttcg tgatagccaa tctgaacgcc cacctgaccg gggtattccg ctgccgccgc
    12001 aaggaaacct tcctgcagca ccggatcgcc cgcgccgagt agcgccagct gcccgccctg
    12061 ctccagaaga cccggtaagg cttccagcac caggtcgaga cctttctggc tggtcagacg
    12121 gctcaccact gcaaaaagcg gcactttatc gtcaacctta agccccattg cgatttgtaa
    12181 ctggcgctta ttttccgctt tatcttccaa cgtatcgcgg gtgtaacgcg aggccaacag
    12241 taagtccgtc tctggactcc agattttctc gtccacgccg ttcagtacgc cggaaagacg
    12301 cccttcacgg tgacgctgtt gcaacagacc ttccataccg taggcaaact gcggttcggt
    12361 gatctcgcga gcgtaggttg gactgaccgc cgtaatgtga tcggcatagt acagaccggc
    12421 cttcaggaaa gagatttgtc cgttgaattc cagcccatga atattaaaga atgaccatgg
    12481 caattggatg tcattcatat gatgtgcata aaacatgcct tgataggcca ggttgtgcac
    12541 agtaaacacc gacttcgccg gacgcccgcg cgccgccaga tacgcaggcg caaggcctgc
    12601 atgccagtcg tgcgcatgca ccacatcagg acgccagaat gggtcaagcc cgctggccat
    12661 ttctgcccca acccacccca gcagcgcaaa acgcaatacg ttgtcggtat aggcaaataa
    12721 gttggtatcg tgatacgggc ttcccggacg atcatagaga tgcggcgcgt caatcaggta
    12781 tatgccaacc ccgttgtaat gaccgaacaa cagcgtgata tgtccggcga aggtatcacg
    12841 acgggatact acctgcgcat cggtaacgcc acggcgaata tcgggaaatg caggcaacag
    12901 tacgcgagcg tcaacgccgt ctgcgatttg tgctgcgggt aatgccccaa taacatcagc
    12961 cagaccgccg gttttaagca gcgggaacat ctctgaacat acatgtaaaa cctgcattat
    13021 cgctcctgtt tatgccctaa cttccgtagc atttcgcgcg ttaccagcac gatgccttct
    13081 tctgaacgat agaaacgacg tgcatcttcc tctgcgtttt caccaatcac catgccttcc
    13141 ggaataacac aagcacgatc gatgacgcag cggcgcagac ggcacgagcg acctacccat
    13201 acttccggta acaatacggc ggaatcaatg ttgcagaatg aattcacgcg aacgcgcgag
    13261 aacagaacgg actgcaccac caccgaaccg gagatcacac agccgccgga taccagtgag
    13321 ttaagggtca tcccgtggct accggagcga tcctgcacga atttcgctgg cggtaatgat
    13381 tcattgtagg tgcgaattgg ccaattgcga tcgtacatat ccagttccgg caccacagag
    13441 gccagatcga ggttcgcttt ccagtaagct tccagcgtac ccacatcgcg ccagtacggc
    13501 tcggcatccg ggtcggattg tacgcaagag agcgggaacg ggtgcgcata ggccagaccg
    13561 gcttcggtga tcttgggaat caaatctttg ccaaagtcgt ggctggagtt ctcatcacga
    13621 tcgtcttctt ccagcagttc atacagatag tcggcgtcaa agacgtagat acccatactc
    13681 gccagagatt tgctcggatc gttcggcatt gacggtgggt tagcaggttt ttcaacgaat
    13741 tcgataattt tatcgttctc atcaaccgcc ataacgccaa atgcggaggc ttcttcaatc
    13801 ggtactggca tacaagcaac ggtgcaacgt gcgccttttt cgacgtgatc gataagcata
    13861 cgcgagtagt cttgcttgta gatatggtcg cccgccagga tcaccacgta ttccgcttta
    13921 taacggcgga taatgtcgag gttttgggtg accgcatctg cggtgccgcg ataccagttt
    13981 tcccctttca ttctctgctg tgctggcagc agatcgacaa actcgttcat ttcttcattg
    14041 aagaatgacc agccgcgctg aatgtgctgc accagagtgt gggactggta ctgggtgatc
    14101 acgcccatac gacggatccc ggagttgatg cagttagaca gcgcaaagtc gataatgcgg
    14161 aacttaccgc cgaagtgtac ggccggtttt gctcgcttat tggttaaatc cttcaggcgg
    14221 gtaccacgtc ctcccgccag tatcagggca acagatttca atggcagctg gcgcgccaac
    14281 attaagtgat cgttcttctc taaactaacc atgactaact ccttttttat catctctgga
    14341 acacacacaa tccgtgtgca ggtccctgcc agacagccgt aatcactggg ttatcctctc
    14401 cagcgaatgg gggaatggcg tgccactccc cagcaggtaa aacaatctct gttacctcaa
    14461 gcgtggcgtt aattgcgatc aaaaagcgat ccgagagcag aatttgcagc tgtttcggcc
    14521 cgttttgcca ctcatccgtg cttaaaggtt gagcatatcg atttagccaa cggacattgc
    14581 cgtcgccttc ttcccaccag cgattctcca ccaaagcggg aatgcgcttg cgcagatgga
    14641 ttaacgcggc ggtaaatgcg gttaaaccac tgcttgcctg cgaccagtcc aaccaggtta
    14701 attggttatc ctgacagtag gcattgttat tgccatgctg gctgtgacca tgttcgtcac
    14761 cggccagtaa catcggcgta ccctgggaga gcaacaacgt tgttaacagg gcgtgaatgc
    14821 tgtcgcgccg ccgttcaacc aggtcaagag aaccgcctaa cccttcttta ccatgattgt
    14881 tactgtaatt gttgttggtc ccgtcgcgat tttcttctcc gtttgcttca ttgtgtttat
    14941 ggttgaagca aacgcagtcg cgaagcgtaa aaccgtcatg cgcggtgacg agattaatcg
    15001 cggcactcgg cagacgacca ttacgtttaa aaacatcgct ggaggcagca aaacgcccgg
    15061 caaacgcccc cagaggcaaa tcataatgta gccagaaacg acgggcagca tcgcggaaat
    15121 gatcgttcca ctcggcaaac agcggcggga aatttcccac ctgataacca ccaggagcga
    15181 tatcccacgg ttcagcaatt aacttcacct gcgagagcac cgggcagttc tggatagcgg
    15241 taaacaacgg cgcatcctga cggaactctg gcgtacggcc catgactgcc gccagatcaa
    15301 agcggaaacc atcgacgtgg caggtttcta cccaataacg caggcaggcg ctggcataat
    15361 ccaccaccgc cggatgactc aaattgagcg tgttgccgca accggtccag ttgtgataat
    15421 cgccgtcttc tcttatccaa taatagctac ggttatcgat cccacgcagc gagaataacg
    15481 ggccgtcgag gtccagttcc gcactatggt tgagcacgat atcaagaatg acttcgatac
    15541 ccgctttatg cagtgctttg attgcatcgc gaaactcatc cagcgccgtt tctggcgagc
    15601 aggcatacgc cggatgcagc gcaaacatcg ccaccgggtt gtaaccccag tagttactta
    15661 gccccatgcg ttgcagacgt ggttcactgg caaactgcgc cactggcagc agttccagcg
    15721 cggtaatgcc caattgtttc aaatagttga tcatcaccgg atgcccgagg gctttataag
    15781 tgccacggat ctcgaccggg atctccgggt gcaagtacgt taatcctttg acatgggctt
    15841 cataaatgat ggtgctgccc cacggcgtgc gcggcggggc atcatcttcc cagtcatagt
    15901 gatcaaccac cactacgcat ttcggcgcaa tggcggcgtt gtcgcgatag tcaggttcat
    15961 tatgaccggc gtgcagcagc gggttatctt taaactcccc ctcaatttgc cgcgcgcaag
    16021 gatcaatcaa caacttcgcc gggttaaagc gatgcccctc ggcgggttgc caggggccat
    16081 gaacgcgata accataacgc aaacccgggc gcgcatccgg cagataaccg tgccaaatgt
    16141 cgccactgtg ccctggcaag tcatagcgat gttcctggcc attggcgtca aagacacaca
    16201 gttctacccg ctcggcatga gcggagaaaa gtgtgaagtt gacgccctga ccgtcgtaat
    16261 gcgcgccgag gggagcgggt ttgccaatgg cgagttgtgt cattctgcct cccgaaccag
    16321 ccagatagtg gccagcggtg gtagcgtcag gcttagtgaa tgctgacgac cgtggctggc
    16381 aatctcatcg ctgtgtaccg tgccgccatt gcctgcatta ctgccgtgat agtgcatgga
    16441 atcggtattg aggatttcac gccatttgcc cggctggttt atgccgaagc gataatcatg
    16501 acgcggcacc ggcgtaaagt tactggcaac gatgatttcg ttaccctctt tatcgcgacg
    16561 cacaaagatc agcaccgagc gttctttgtc atccaccacc agccattcaa agccgtacgg
    16621 gtcaaaatcc agttcatgca ttgctttatg gtggcggtag gtgaggttca gatcgcgcac
    16681 cagacgctgg acaccgtggt gccagttatc gccgccttcc aacagatgcc agtcgaggct
    16741 ggcgtcatgg ttccactcgc ggccctgggc aaattcgtta cccatgaaca gtagtttctt
    16801 gcccgggaat gcccacatcc agccatagta ggcgcgcagg ttcgcgaatt tctgccatgc
    16861 gtcgcccggc atgcggtcga gaatcgattt tttaccgtgg accacttcat catgcgacaa
    16921 cggcaggacg aagttttcag tgtagttgta gagaatcccg aaggtcagtt tatcgtgatg
    16981 atactgacga taaaccgggt cgagcttcat gtagtccagg gtgtcatgca tccagccgag
    17041 gttccacttg taccagaagc ccagaccgcc catatcctgc ggacgagaaa cgccagggaa
    17101 atcggtagac tcctcagcca ttgtcaccgc accggaaacc tgctcaccaa gaatacggtt
    17161 ggtattacgc aagaattcaa tcgcttcaag attctcgcgc ccgccaaatt cgttcgggat
    17221 ccactccccc tctttacggc tgtagtcgcg ataaatcatt gacgccaccg catcgacgcg
    17281 cagcgcatca ataccaaaac gttcaatcca gtaaagcgcg ttaccgacga ggaagttact
    17341 gacttcacgg cgaccatagt tgtagatcag cgtgttccag tcctgatgat agccttcacg
    17401 cggatcgctg tgttcataca agttcgtgcc atcaaattcg gcaagcgcaa agtcatcagt
    17461 cgggaagtgg cctggcaccc agtcgagaat cacgttcaga ccagctgcgt gtgcggcatc
    17521 aatgaaataa cggaagtcgt cgcgagtacc aaaacggcgg gttggtgcat acaggccggt
    17581 tggctgataa ccccaactgc catcgaaggg atgctcgtta atgggcagta gttcgaggtg
    17641 ggtaaagccc atccatttag cataaggcac cagttgatcg gccagctcgc ggtagctcaa
    17701 ccagaaattg ttgtcggtgt ggcgacgcca ggaacccagg tgaacttcat aaatagagat
    17761 tggcgcatca aactgattcg cttttttgcg ctcttcagtc tgtacaacct tttccggcag
    17821 cccgcaaata agagacgcgg tttccgggcg catttgcgct tcgaaggcat aagggtcgga
    17881 cttcagacgc aagttgccat tggcatcaat catctcgtat ttatagagct gaccgttatg
    17941 cgccccaggg ataaacagtt cccagatgcc gctctcttta cgcaggcgca tcgggtgacg
    18001 gcgaccgtcc cagtagttga attgcccaac caccgagacc cgacgggcgt ttggagccca
    18061 gacagagaaa cgcgtacctg tgacgccatc catagtatct gcatgcgcgc ctaaggtttc
    18121 atacgggcgc aggtgagtac cttcagataa tagccaggca tccatttcct ggattagcgg
    18181 accaaaacgg taaggatcat caatcaggtt ttgctgacca tgccagacaa cagccaactg
    18241 atagcggaaa aaattcttac gtcgcggaat gacgccgcta aagaatcccc gtgagtcgag
    18301 acactccagt tttgcgagtt tgcgcccggt tttcggttca atcacccaca catcggtagc
    18361 gtcgggtaaa agggcacgga cttccagtcc cgcggtggtt ttatgcattc ccagtacgga
    18421 aaaaggatcc gcaaaatggc ctgcaattag cgcgttaatc acgtctctat cgatacgatc
    18481 ggacatgctt gtcttcctgt tttattgtgt caccccatcc aattggattt ttgacttctg
    18541 gttgtgacat ttttttgacc tgaacggcgc agcactctgt gcatcctctc tgcgtcgtcc
    18601 tcacttcagg taaggctgtg aatactcatg tattcagcca cccttaaaga atagccaatg
    18661 ctctatttaa ctcccggtaa atcatgaaac atctgcgctt actcctgtat tacgcactaa
    18721 caggggcggc atcgcgcccc agatttaatg aataaagatt acgccagttg acgaagcatc
    18781 cgacgcagcg gctccgcggc cccccacagc agctggtcgc ccacggtaaa ggctgacagg
    18841 aactctggtc ccatattcag cttacgcagg cggcctaccg gcgtggtcag cgtgccggta
    18901 acggcagctg gggttagctc acgcatagtg atttcccgat cgttcggaac gactttcgcc
    18961 cacggattgt gcgcagccag cagttcttcc acggtcggaa tagacacatc ttttttcaat
    19021 ttaatagtga atgcctggct gtggcagcgc aatgccccga cacgcacaca taaaccatct
    19081 accggaatta cggaagatgt gttgaggatc ttgttggttt ccgcctgccc tttccactct
    19141 tcgcggctct gaccgttatc gagctgtttg tcgatccacg gaatcaggct acccgccagc
    19201 ggcacgccaa agttatccac cggcagctca ccgctacggg ttaaggttgt gactttgcgt
    19261 tcgatatcga gaatagcaga ggacggggtc gcgagttcat ctgccacatg gccatacaga
    19321 tggcccatct gggttaataa ctcacgcata tgtcgcgcac caccgccgga agcggcctgg
    19381 taggttgcaa cggacaccca atcaacaaga tcattggcga ataaaccacc caacgacatc
    19441 aacatcaggc ttacggtaca gttaccgcca acaaaagtcc tgatgccatt atttaatccg
    19501 tcggtaatga cgtcctgatt gacggggtca agaatgatga tggcgtcatc tttcatgcgc
    19561 agagacgatg ctgcgtcaat ccagtaacct tgccatccgc tttcacgaag ctttggatag
    19621 atttcgttgg tataatcgcc gccctgacag gtcacaatga tatcgagggc ctttagcgcc
    19681 tccagatcaa aggcatcctg aagtgtgcca gtggttccgc caaaagacgg cgcagcctgg
    19741 ccaagctgag aagtagaaaa gaagacaggg cgaatggcgt cgaagtcgcg ctcttcaacc
    19801 atgcgttgca tgagaacgga gccgaccata ccgcgccagc cgataaaacc aacatttttc
    19861 ataagcgttt ttttcctgca aagatgtgtg ctgtataaat gtgccggtct cctcttggca
    19921 catctttcac catacaaaaa gcagccaaag tcgcaagtga aattaatcaa tgatagcgaa
    19981 gccatcagta atgcgactta tcctgctttg ttagcacgca gaaagtccgc ggcaattatc
    20041 agggaatttg agttatgaat gaaatcattt ctgcagcagt tttattgatc ctgattatgg
    20101 atccgctcgg aaacctacct attttcatgt ccgtactgaa acatactgaa ccgaaaagac
    20161 ggcgggcaat catggtgcga gagttgctta ttgctctcct ggtgatgctg gtgttcctgt
    20221 ttgcgggtga gaaaattctg gcatttctta gcctacgagc agaaaccgtc tccatttctg
    20281 gcggcatcat tctgtttctg atcgccatta aaatgatttt ccccagcgct tcaggaaata
    20341 gcagcgggct tccggcaggt gaagagccat ttatcgtgcc gttggcaatt ccgttagtcg
    20401 ccgggccgac tattctcgcc acgctgatgt tgttgtctca tcagtacccg aatcagatgg
    20461 ggcatctggt gattgctctg ctgctggcct ggggcggcac ctttgtcatc ctgctacagt
    20521 cttcgctatt tttacgtctg ctgggcgaga aaggggtgaa cgcacttgaa cgcctgatgg
    20581 gattgattct ggtgatgatg gcaacccaga tgttcctcga cggcattaga atgtggatga
    20641 aggggtaacg aggagagttt ttcgaaaaga acccggtagc gtcacaacta ccgggcgaac
    20701 aaacttaact caacagctgg aacgcaatca tcccaacgat ggcaccgaca gtgccgagga
    20761 tggtttccat catggtccag gttttcagcg tttcggcttc ggtcgcgccg gtaaatttac
    20821 cgaacaacca gaaaccggcg tcgttaacgt ggctgacaac aatcgaacca ccagcgatac
    20881 aaatcgacag cgccgccatt tgcgcaccgg agtagttcag ttgttcaata accggcatca
    20941 ccagtcctac cgccgttaaa caggctacgg tggcagaacc ctgaatgatg cgcactgcag
    21001 ctgccagcac gaagcaggtg atggctatcg gcaggcccat gccggttaac gcttcgccca
    21061 gtgccggacc tacgccagag tcaaccagca cctgtttgaa cacgccgcct gcaccaatca
    21121 ccagcagaat gatccccgcc ggttgcagcg cgtgaccgca aatctccatc actttgtctt
    21181 ttggcatgcc ctgacgcatt gccaggccat aaatcgctac cagacaagca accagaatcg
    21241 cggtaaacgg atgaccaata aactcgaacc attcgtaagc ggtagagcct tctggcacaa
    21301 aacgcgcggc aatggttttc agccccacca gcaccagcgg caacaggatc agcgacaggc
    21361 tgaatccgaa agatggcatt ttgccttcgc cgagatgcgg ttcgctgatg tcgtcaggaa
    21421 tatgcaactc cacgtagcgg ctgatgaaat taccccacag cggcccggca ataatcattc
    21481 ccggaattgc cgcacacagg ccaatcagga tcatccagcc aaagtcggca ttcatctgcg
    21541 atgccagcag catcggcgct ggtccaggca ccagaaatgc cgccgctgcc gccacgcctg
    21601 caaataatgg gattaccagc ttcaccaggt tcgtaccggt gtggcgcgcc attgagaaag
    21661 caacgctaat cagcagaact atcgccactt caaagaacag cggtagcgca cagaccagcc
    21721 ccgcaaggcc gatggcataa tgcgcgcggc tgtgaccgaa ggatttgagc attttgacgg
    21781 caatctgatc gactgcgccg gtttcatgta agatcttgcc aaacatagct cccagggcga
    21841 caaccaccgc caggaagccg agggtgcctc ccatcccttt ttccatcgtc gctgcgattt
    21901 tatcgagcgg cataccggaa aaaaggccag cccccatgga caccaccatc aaagccagga
    21961 aagcgtgcat acgcgccttc atgactaaaa acagcagcag taaaacagac cctactgctg
    22021 ttaaaacaag cgttaatgta gtcactactt atttgccttt tttaataacc tcaatggtgc
    22081 ttgccacaac accttccagc ggttgatcga tatccaccat cagtacatcg gtttcgtccg
    22141 cacccggctc ctgcagcgtt tcaaactgcg tcaccaacat ttgggtttta aagaaatggc
    22201 ctttgcgcgc tttcaggcgg ctttcaatca catcaaaatc gcctttcagg tagatgaaag
    22261 agagattcgg attaccttca cgcagcaagt cgcgatagtg ttttttcaat gcagaacaga
    22321 cgatcagtga cactttatta gtgcgctgca tagcaaacgc ggcgtcgttc agcgcctgca
    22381 accacggttt gcgatcgtcg tcattcagcg gttcgccaga cgccattttt tcgatattgc
    22441 gccgtggatg gaggaaatcg ccatcaagaa acgcggcatg aagttgatgc gccacttcac
    22501 tggcgaccgc agatttgccg ctgcctgaga cgcccatcaa gacgtaaatg tggtgatcat
    22561 ggttagtcgt gctcaaagtg gtgcccccac aatacaagaa ttaaaaatgt tacgggtaac
    22621 tgttatcggt aacattgtcc ggccggacaa tatcagaagc aatatccatc cgtgccttaa
    22681 gtgtataagt gtgagctact tcaaatttgt gggcttaaat agatccgccc ggtgacaagg
    22741 tgaaacctaa atctaacatt ttcggtgtca cagattcgcc acgaatacgc gccagcaggc
    22801 gttcagcgcc aatactgccc atccgctcac gcggcgtcag cacgctcgcc agtcgtggct
    22861 ccatcacctg accaatgtca tgaccgtgga aaccggcaat cgccatatcg tcaggaactt
    22921 ttaaccccag acgctgacat tcaaacgccg cgccgaccgc cagatcatca ttagtacaga
    22981 acacgccatc cagctgcgga tattcccgcc tcgcctggcg aatcagttca ataccggaag
    23041 agtaagaaga agattgctca accatcacgc tatatggcac caggtctgca tccagcatcg
    23101 cctgttcgta tcccttctgt ttgatgatag tacgttcgtc gagacgtgcg ccgagataag
    23161 caatgtggcg atgcccgcga gcaataatgg cggtggtcat ctggcgtgcc gcttcaaagt
    23221 tatcaaaacc gacggcaata tcgaggcacg gcgacttgct gtccatcaac tccaccaccg
    23281 ggatccccgc cacttcaatc atctttaagg tgcgtggcgt gtgagtacgt tcggtgagga
    23341 tcagtccatc gatattccat gagagcatgg attcgaggcg ttcttgctcc atttccggtt
    23401 tataaccgta gtgcgccagc atggtttgat aaccgtgcgc gtcggtgacg ctttcgattc
    23461 cgcgtaatac ttccgcgaaa acctggttgg tgagagaagg taacaggacg ccaatcgccc
    23521 ggctggtggc gttagagagg atatcgggcg cacgattggg aatataaccc agttcatcaa
    23581 gagcggcggc aatcttgccg cgtagagcga cggaaacctg ctccgggtta cgcaaaaagc
    23641 ggctgaccgt cattttggtc acgcctacac ggtcagccac atcctgaagt acgggtcttt
    23701 tctttttcat cgtcctgaag gtacaaaaga gatagattct cttagtttaa cacggacgca
    23761 tcagaacctt cccggggaaa acagtgcttg tttacaatta ttagatatac actaaatcaa
    23821 ttgcatcgca ttgtgctaac gacgcggcaa aaccccggaa gcgtgcatca gcacgcgacc
    23881 ggagcgagcg aggaagcctg caaaaaggca ggcttcaaaa atgccgttga gttttaaacc
    23941 ggcggcagat cgaacagtaa cacttcgctg tcgctatccg catggatgga gattgcctgc
    24001 tcatcccaga ttgccagacc atcgctggtc gaggctttca cgccgttaat ggtgacattg
    24061 cctttcacca cctggatcca gacgcggcgt tcagcggcaa tctgatgcac cgactgctca
    24121 tctttcagca acgcccaacg atacagttcc atatcctgat gcactttcag cgaaccatct
    24181 cgcgcatccg gcgagagcac cagctgtttg ccctgcacgg catcgaagcg acgctgttca
    24241 taacgcggcg taatgccatt ttcttcaggc atgatccaga tctgatacag atgcagacgc
    24301 tcggtgctgc ttgggttgta ctctgagtga cgaatacccg tacccgcact cataatctgg
    24361 aactcacccg ccggaacctg ctctttattg cccatgctgt cctgatgctc aacagtacct
    24421 tccagcacgt aggtcaaaat ttccatatct ttatgcgggt gagtgccgaa gccctgccct
    24481 gcttcaatca cgtcgtcgtt aatcacgcgc agcgcggaga agcccataaa gttcggatcg
    24541 taatagttgg caaaagagaa agtatgccag gagtccagcc agccatgatt tgcatgacca
    24601 cgttcatttg ctttgcgtaa gtagatcatt tgtattcgcc ccctgaatga tttcgatggg
    24661 cttagtgtgg acccgatctg cctgggatga tagagggtga aaactgaccc ctctgttcaa
    24721 aaaaattgaa ctattcgagg ggccatcaaa cttacttagc gagagttact gtggagggag
    24781 aggcttgctc aaatccgcgt tcaaggattt ccagattggt aagaacttca gattccttga
    24841 cgtaatttgg cgcaccgtgg gtgatggttt gatacaacgc atcataaacg cgcccgtaat
    24901 cgcccatctc cggcttcatc tcttctctga ccgtcacgcc ctcgtcattg acatactcca
    24961 gcacaccgac cgaattatcc gctgcgaatc ccggttcgcc cggcataata ttagccttca
    25021 ggctggtttc ctgctggtcg ataccgtatt taataaacga acctttctta ccgtgaacga
    25081 taaatttcgg ataatcgatt ttcaccagat ggctggtttt gacgatggct ttcaggtcgc
    25141 cataaaacag ttgcgcttca aaggtgtcgt caggattggc tttattacgc aggctgcgga
    25201 tgtcataagc gacgtgatcc gggcgaccga acagagaaat aatctggtcc atcgtatgca
    25261 caccaaggcc ataaaacgcg ccatcctgcg gcagcccagg tttggtttct gccaccgggc
    25321 ggtaatagtc aaaatggctt tccacttcaa caatctctcc caacttgcca ctttcaatcg
    25381 cttttttcgc tgtcaggaag caggagtcaa agcgacgatt ctgatacggc gtgacggtca
    25441 gccctttgct tttcgccaac gcaaacagct ctttcgcctg cgcaagtgtc ggagtgaacg
    25501 gtttttcgac cagcacattt ttcccggctt ccagcgcgcg cttcgcgtac tcgaagtggc
    25561 tgtccgcgtg ggtgcagaca acaaccagct taacatcggg atcgtttagt acttcgtcga
    25621 gatcgctggt gaaatggata tgggaataaa tgggagcctg ttcttccggc ttcgcatggc
    25681 gacgaaaaat atgcgcgaca tgccaactat ccttgcggtt aagtacatac ggcagatggt
    25741 aacgggtggt gcttttgccg aagccaataa aggcgcagtt gatgaccatg atttcgtcct
    25801 ttttaaggtg gttatttaca ccttagcgca aagcggacgt ggttcctact ggagtgcgca
    25861 taacgaacac aagcactccc gtggataaat tgagaacgaa agatcaaaaa aaaagccagc
    25921 acccggctgg ctaagtaata ctggaagcaa tgtgagcaat gtcgtgcttt caggttctcc
    25981 gcgagggtct tcctgatcgc gagacaataa taatcattct cattcgcact tgtccaacac
    26041 tttttgcaaa aaaatgcatt tgactcgcat ttgaaagtca atgatgttga aagggacatt
    26101 taccccaaag aggacaaagg aatgagtgag atagtaatac gccacgcaga aacacgggat
    26161 tacgaggcca tcaggcagat tcacgcccag ccggaggtgt attgcaacac actacaggtg
    26221 cctcatcctt ccgatcatat gtggcaggag cgactcgccg atcgtcccgg catcaagcaa
    26281 ctcgtcgcct gtattgatgg agacgtcgtg ggccatctca ccattgacgt gcaacagcgc
    26341 ccacgccgca gtcatgttgc cgattttggt atctgtgtcg actctcgttg gaagaaccgc
    26401 ggcgtcgcca gcgccctgat gcgagagatg attgaaatgt gcgacaactg gttgcgggta
    26461 gatcgcattg aactaaccgt gtttgtcgat aacgccccgg caattaaggt ctataaaaaa
    26521 tacggctttg aaattgaagg gactggtaag aagtacgcat tgcgtaatgg tgaatatgtc
    26581 gatgcatatt atatggcgcg ggtgaagtaa gatagtgccc tttttctgag atggaaaaag
    26641 ggtgtcattc aaaatcgaca taccttcctt taaggtattt atttgcccaa tacatatatt
    26701 gagatttaac tcataattac ttcctaaagt gtaatattt
//
LOCUS       DAZGAN020000061        26630 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGAN020000061 DAZGAN020000000
VERSION     DAZGAN020000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.061"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..588
                     /locus_tag="ACHKYO_RS14060"
                     /old_locus_tag="pgaptmp_002812"
     CDS             <1..588
                     /locus_tag="ACHKYO_RS14060"
                     /old_locus_tag="pgaptmp_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HGU4429630.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            771..965
                     /gene="bfd"
                     /locus_tag="ACHKYO_RS14065"
                     /old_locus_tag="pgaptmp_002813"
     CDS             771..965
                     /gene="bfd"
                     /locus_tag="ACHKYO_RS14065"
                     /old_locus_tag="pgaptmp_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HGU4429631.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1038..1514
                     /gene="bfr"
                     /locus_tag="ACHKYO_RS14070"
                     /old_locus_tag="pgaptmp_002814"
     CDS             1038..1514
                     /gene="bfr"
                     /locus_tag="ACHKYO_RS14070"
                     /old_locus_tag="pgaptmp_002814"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HGU4429632.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1511..1978)
                     /locus_tag="ACHKYO_RS14075"
                     /old_locus_tag="pgaptmp_002815"
     CDS             complement(1511..1978)
                     /locus_tag="ACHKYO_RS14075"
                     /old_locus_tag="pgaptmp_002815"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGU4429633.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACHKYO_RS14080"
                     /old_locus_tag="pgaptmp_002816"
     CDS             2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACHKYO_RS14080"
                     /old_locus_tag="pgaptmp_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGU4429634.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2701..3330
                     /gene="rplC"
                     /locus_tag="ACHKYO_RS14085"
                     /old_locus_tag="pgaptmp_002817"
     CDS             2701..3330
                     /gene="rplC"
                     /locus_tag="ACHKYO_RS14085"
                     /old_locus_tag="pgaptmp_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGU4429635.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3341..3946
                     /gene="rplD"
                     /locus_tag="ACHKYO_RS14090"
                     /old_locus_tag="pgaptmp_002818"
     CDS             3341..3946
                     /gene="rplD"
                     /locus_tag="ACHKYO_RS14090"
                     /old_locus_tag="pgaptmp_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGU4429636.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3943..4245
                     /gene="rplW"
                     /locus_tag="ACHKYO_RS14095"
                     /old_locus_tag="pgaptmp_002819"
     CDS             3943..4245
                     /gene="rplW"
                     /locus_tag="ACHKYO_RS14095"
                     /old_locus_tag="pgaptmp_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGU4429637.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4263..5084
                     /gene="rplB"
                     /locus_tag="ACHKYO_RS14100"
                     /old_locus_tag="pgaptmp_002820"
     CDS             4263..5084
                     /gene="rplB"
                     /locus_tag="ACHKYO_RS14100"
                     /old_locus_tag="pgaptmp_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGU4429638.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5101..5379
                     /gene="rpsS"
                     /locus_tag="ACHKYO_RS14105"
                     /old_locus_tag="pgaptmp_002821"
     CDS             5101..5379
                     /gene="rpsS"
                     /locus_tag="ACHKYO_RS14105"
                     /old_locus_tag="pgaptmp_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGU4429639.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5394..5726
                     /gene="rplV"
                     /locus_tag="ACHKYO_RS14110"
                     /old_locus_tag="pgaptmp_002822"
     CDS             5394..5726
                     /gene="rplV"
                     /locus_tag="ACHKYO_RS14110"
                     /old_locus_tag="pgaptmp_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGU4429640.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5744..6445
                     /gene="rpsC"
                     /locus_tag="ACHKYO_RS14115"
                     /old_locus_tag="pgaptmp_002823"
     CDS             5744..6445
                     /gene="rpsC"
                     /locus_tag="ACHKYO_RS14115"
                     /old_locus_tag="pgaptmp_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGU4429641.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6458..6868
                     /gene="rplP"
                     /locus_tag="ACHKYO_RS14120"
                     /old_locus_tag="pgaptmp_002824"
     CDS             6458..6868
                     /gene="rplP"
                     /locus_tag="ACHKYO_RS14120"
                     /old_locus_tag="pgaptmp_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGU4429642.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6868..7059
                     /gene="rpmC"
                     /locus_tag="ACHKYO_RS14125"
                     /old_locus_tag="pgaptmp_002825"
     CDS             6868..7059
                     /gene="rpmC"
                     /locus_tag="ACHKYO_RS14125"
                     /old_locus_tag="pgaptmp_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGU4429643.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACHKYO_RS14130"
                     /old_locus_tag="pgaptmp_002826"
     CDS             7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACHKYO_RS14130"
                     /old_locus_tag="pgaptmp_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGU4429644.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7478..7849
                     /gene="rplN"
                     /locus_tag="ACHKYO_RS14135"
                     /old_locus_tag="pgaptmp_002827"
     CDS             7478..7849
                     /gene="rplN"
                     /locus_tag="ACHKYO_RS14135"
                     /old_locus_tag="pgaptmp_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGU4429645.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7860..8174
                     /gene="rplX"
                     /locus_tag="ACHKYO_RS14140"
                     /old_locus_tag="pgaptmp_002828"
     CDS             7860..8174
                     /gene="rplX"
                     /locus_tag="ACHKYO_RS14140"
                     /old_locus_tag="pgaptmp_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGU4429646.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8189..8728
                     /gene="rplE"
                     /locus_tag="ACHKYO_RS14145"
                     /old_locus_tag="pgaptmp_002829"
     CDS             8189..8728
                     /gene="rplE"
                     /locus_tag="ACHKYO_RS14145"
                     /old_locus_tag="pgaptmp_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGU4429647.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8743..9048
                     /gene="rpsN"
                     /locus_tag="ACHKYO_RS14150"
                     /old_locus_tag="pgaptmp_002830"
     CDS             8743..9048
                     /gene="rpsN"
                     /locus_tag="ACHKYO_RS14150"
                     /old_locus_tag="pgaptmp_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGU4429648.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9082..9474
                     /gene="rpsH"
                     /locus_tag="ACHKYO_RS14155"
                     /old_locus_tag="pgaptmp_002831"
     CDS             9082..9474
                     /gene="rpsH"
                     /locus_tag="ACHKYO_RS14155"
                     /old_locus_tag="pgaptmp_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGU4429649.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9487..10020
                     /gene="rplF"
                     /locus_tag="ACHKYO_RS14160"
                     /old_locus_tag="pgaptmp_002832"
     CDS             9487..10020
                     /gene="rplF"
                     /locus_tag="ACHKYO_RS14160"
                     /old_locus_tag="pgaptmp_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGU4429650.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10030..10383
                     /gene="rplR"
                     /locus_tag="ACHKYO_RS14165"
                     /old_locus_tag="pgaptmp_002833"
     CDS             10030..10383
                     /gene="rplR"
                     /locus_tag="ACHKYO_RS14165"
                     /old_locus_tag="pgaptmp_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGU4429651.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10398..10901
                     /gene="rpsE"
                     /locus_tag="ACHKYO_RS14170"
                     /old_locus_tag="pgaptmp_002834"
     CDS             10398..10901
                     /gene="rpsE"
                     /locus_tag="ACHKYO_RS14170"
                     /old_locus_tag="pgaptmp_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGU4429652.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10905..11084
                     /gene="rpmD"
                     /locus_tag="ACHKYO_RS14175"
                     /old_locus_tag="pgaptmp_002835"
     CDS             10905..11084
                     /gene="rpmD"
                     /locus_tag="ACHKYO_RS14175"
                     /old_locus_tag="pgaptmp_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGU4429653.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11088..11522
                     /gene="rplO"
                     /locus_tag="ACHKYO_RS14180"
                     /old_locus_tag="pgaptmp_002836"
     CDS             11088..11522
                     /gene="rplO"
                     /locus_tag="ACHKYO_RS14180"
                     /old_locus_tag="pgaptmp_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGU4429654.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11530..12861
                     /gene="secY"
                     /locus_tag="ACHKYO_RS14185"
                     /old_locus_tag="pgaptmp_002837"
     CDS             11530..12861
                     /gene="secY"
                     /locus_tag="ACHKYO_RS14185"
                     /old_locus_tag="pgaptmp_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGU4429655.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACHKYO_RS14190"
                     /old_locus_tag="pgaptmp_002838"
     CDS             12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACHKYO_RS14190"
                     /old_locus_tag="pgaptmp_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGU4429656.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13156..13512
                     /gene="rpsM"
                     /locus_tag="ACHKYO_RS14195"
                     /old_locus_tag="pgaptmp_002839"
     CDS             13156..13512
                     /gene="rpsM"
                     /locus_tag="ACHKYO_RS14195"
                     /old_locus_tag="pgaptmp_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGU4429657.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13529..13918
                     /gene="rpsK"
                     /locus_tag="ACHKYO_RS14200"
                     /old_locus_tag="pgaptmp_002840"
     CDS             13529..13918
                     /gene="rpsK"
                     /locus_tag="ACHKYO_RS14200"
                     /old_locus_tag="pgaptmp_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGU4429658.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13952..14572
                     /gene="rpsD"
                     /locus_tag="ACHKYO_RS14205"
                     /old_locus_tag="pgaptmp_002841"
     CDS             13952..14572
                     /gene="rpsD"
                     /locus_tag="ACHKYO_RS14205"
                     /old_locus_tag="pgaptmp_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGU4429659.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14598..15587
                     /gene="rpoA"
                     /locus_tag="ACHKYO_RS14210"
                     /old_locus_tag="pgaptmp_002842"
     CDS             14598..15587
                     /gene="rpoA"
                     /locus_tag="ACHKYO_RS14210"
                     /old_locus_tag="pgaptmp_002842"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGU4429660.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15628..16011
                     /gene="rplQ"
                     /locus_tag="ACHKYO_RS14215"
                     /old_locus_tag="pgaptmp_002843"
     CDS             15628..16011
                     /gene="rplQ"
                     /locus_tag="ACHKYO_RS14215"
                     /old_locus_tag="pgaptmp_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGU4429661.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            16118..16486
                     /locus_tag="ACHKYO_RS14220"
                     /old_locus_tag="pgaptmp_002844"
     CDS             16118..16486
                     /locus_tag="ACHKYO_RS14220"
                     /old_locus_tag="pgaptmp_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HGU4429662.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16497..16922
                     /gene="zntR"
                     /locus_tag="ACHKYO_RS14225"
                     /old_locus_tag="pgaptmp_002845"
     CDS             16497..16922
                     /gene="zntR"
                     /locus_tag="ACHKYO_RS14225"
                     /old_locus_tag="pgaptmp_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HGU4429663.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16978..17196
                     /gene="arfA"
                     /locus_tag="ACHKYO_RS14230"
                     /old_locus_tag="pgaptmp_002846"
     CDS             16978..17196
                     /gene="arfA"
                     /locus_tag="ACHKYO_RS14230"
                     /old_locus_tag="pgaptmp_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HGU4429664.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(17193..17603)
                     /gene="mscL"
                     /locus_tag="ACHKYO_RS14235"
                     /old_locus_tag="pgaptmp_002847"
     CDS             complement(17193..17603)
                     /gene="mscL"
                     /locus_tag="ACHKYO_RS14235"
                     /old_locus_tag="pgaptmp_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGU4429665.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17733..19109)
                     /gene="trkA"
                     /locus_tag="ACHKYO_RS14240"
                     /old_locus_tag="pgaptmp_002848"
     CDS             complement(17733..19109)
                     /gene="trkA"
                     /locus_tag="ACHKYO_RS14240"
                     /old_locus_tag="pgaptmp_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGU4429666.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(19131..20420)
                     /gene="rsmB"
                     /locus_tag="ACHKYO_RS14245"
                     /old_locus_tag="pgaptmp_002849"
     CDS             complement(19131..20420)
                     /gene="rsmB"
                     /locus_tag="ACHKYO_RS14245"
                     /old_locus_tag="pgaptmp_002849"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGU4429667.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20466..21413)
                     /gene="fmt"
                     /locus_tag="ACHKYO_RS14250"
                     /old_locus_tag="pgaptmp_002850"
     CDS             complement(20466..21413)
                     /gene="fmt"
                     /locus_tag="ACHKYO_RS14250"
                     /old_locus_tag="pgaptmp_002850"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGU4429668.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21428..21937)
                     /gene="def"
                     /locus_tag="ACHKYO_RS14255"
                     /old_locus_tag="pgaptmp_002851"
     CDS             complement(21428..21937)
                     /gene="def"
                     /locus_tag="ACHKYO_RS14255"
                     /old_locus_tag="pgaptmp_002851"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGU4429669.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            22067..23191
                     /gene="dprA"
                     /locus_tag="ACHKYO_RS14260"
                     /old_locus_tag="pgaptmp_002852"
     CDS             22067..23191
                     /gene="dprA"
                     /locus_tag="ACHKYO_RS14260"
                     /old_locus_tag="pgaptmp_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HGU4429670.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            23163..23636
                     /gene="smg"
                     /locus_tag="ACHKYO_RS14265"
                     /old_locus_tag="pgaptmp_002853"
     CDS             23163..23636
                     /gene="smg"
                     /locus_tag="ACHKYO_RS14265"
                     /old_locus_tag="pgaptmp_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HGU4429671.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23665..24207
                     /locus_tag="ACHKYO_RS14270"
                     /old_locus_tag="pgaptmp_002854"
     CDS             23665..24207
                     /locus_tag="ACHKYO_RS14270"
                     /old_locus_tag="pgaptmp_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HGU4429672.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            24212..24784
                     /gene="tsaC"
                     /locus_tag="ACHKYO_RS14275"
                     /old_locus_tag="pgaptmp_002855"
     CDS             24212..24784
                     /gene="tsaC"
                     /locus_tag="ACHKYO_RS14275"
                     /old_locus_tag="pgaptmp_002855"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HGU4429673.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24789..25607
                     /gene="aroE"
                     /locus_tag="ACHKYO_RS14280"
                     /old_locus_tag="pgaptmp_002856"
     CDS             24789..25607
                     /gene="aroE"
                     /locus_tag="ACHKYO_RS14280"
                     /old_locus_tag="pgaptmp_002856"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGU4429674.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25604..25861
                     /locus_tag="ACHKYO_RS14285"
                     /old_locus_tag="pgaptmp_002857"
     CDS             25604..25861
                     /locus_tag="ACHKYO_RS14285"
                     /old_locus_tag="pgaptmp_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HGU4429675.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25837..26391)
                     /locus_tag="ACHKYO_RS14290"
                     /old_locus_tag="pgaptmp_002858"
     CDS             complement(25837..26391)
                     /locus_tag="ACHKYO_RS14290"
                     /old_locus_tag="pgaptmp_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HGU4429676.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6831 a         6324 c         6913 g         6562 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt acacgttaat
      601 tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat ttgagctcga
      661 aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt gctattgtaa
      721 tcataaccat tctcatttac actttgcgca gaatttgaac gggagcaatc atgtacgttt
      781 gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag tttcatccgc
      841 aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt aagtgcattc
      901 gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt aaagagattg
      961 cctgaagacc acgcttattt tttgacatcc cgctagcccc atctacgctc taatagtgga
     1021 agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc aataaactat
     1081 tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg tttaaaaact
     1141 ggggcctgat gcgtctcaat gacgtcgaat accatgaatc catcgatgag atgaaacacg
     1201 ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta caggatttag
     1261 gcaaactggg gattggtgaa gatgtcgagg agatgctgca atccgacctg cgattagaac
     1321 tggaaggcgc gaaggatctc cgtgaggcta tcgcgtatgc cgacagcgtt catgactatg
     1381 ttagccgtga tatgatgatc gagatccttg ccgatgaaga aggtcatatt gactggctcg
     1441 aaactgagct ggatctcatc ggtaaaatcg gtttacaaaa ttacctgcaa tcacaaataa
     1501 aagttaaaga ttaacggcct gctagcaaac tatcccaacc tacaacgaaa cccgcagccg
     1561 ccagaaatgg cccaaacggc agcgggtttt ttaatgactc ttttcctctc atgagcagac
     1621 caacaactac ggctccgcaa gcgaatgagg cagcaaggaa aaccagtcgt ggcaagaagg
     1681 tccaggtatg ccaggcacca agggcggcga gaaacttcac atcaccatag cctaatcctt
     1741 ctttatgacg cagtatgcga tacccccagt aaatgacagc gaatgtgccg taaccaataa
     1801 tcgcccccca taacgcatcg gctaaaccgt caggattaca aacctgagag aacaaaagac
     1861 ctgaccagag taacgggcag gtaaatctgt caggtaacaa gccatgcttt gcatcccata
     1921 aaaaaagtag gacagataaa caagcgtaca aaatcagaaa gggaaggtta gcgtccataa
     1981 aacatcctct gtaattggag tatcaccatc gaataactcc ggatgctcag caacagagaa
     2041 atcgctgatt tgttaacctg gttccggtga aaaacgctat cttcacaacc ttacaaattt
     2101 cgtggaatac agctcgcaat ctgcgcgttg cgattaattt ctacccgatg cttgcgtcag
     2161 gctcagattt gcgtataatg cgcgggcttg tcgtagttga cagcaggttc aatctgaacc
     2221 tcagttagcc gaatttggct acctaacaat gctcccaatc ggggagctac gtaagaacgg
     2281 ttacactctc ccatcaatcg taatgggtct gaggagtaat cattttcgtt tataaaataa
     2341 ttggagctct ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag cgtttgatca
     2401 tcgtctgatc gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca ctggtgcgca
     2461 ggtccgtggt ccgatcccgc tgccgacacg caaagagcgc ttcactgttc tgatctcccc
     2521 gcacgtcaac aaagacgcgc gcgatcagta cgaaatccgt actcacttgc gtctggttga
     2581 catcgttgag ccaaccgaga aaaccgttga tgctctgatg cgtctggatc tggctgccgg
     2641 tgtagacgtg cagatcagcc tgggttaatc aggtcattga gcgattgaga ggttgaaaca
     2701 atgattggtt tagtcggtaa aaaagtgggt atgacccgta tcttcacaga agacggcgtt
     2761 tctatcccag taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt taaagacctg
     2821 gctaacgatg gctaccgtgc tattcaggtg accaccggtg ctaaaaaagc taaccgtgtg
     2881 accaagcctg aagctggcca cttcgctaaa gctggcgtag aagctggccg tggtctgtgg
     2941 gaattccgcc tggctgaagg cgaagagttc actgtaggtc agagcattag cgttgaactg
     3001 tttgctgacg ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg tttcgcaggt
     3061 accgttaagc gctggaactt ccgtacccag gacgctactc acggtaactc cttgtctcac
     3121 cgcgttccgg gttctatcgg tcagaaccag actccgggca aagtgttcaa aggcaagaaa
     3181 atggcaggtc agatgggtaa cgaacgtgta accgttcaga gccttgacgt agtacgcgtt
     3241 gacgctgagc gcaacctgct gctggttaaa ggtgctgtcc cgggtgcaac cggtagcgac
     3301 ctgatcgtta aaccagctgt gaaggcgtaa ggagatagca atggaattag tattgaaaga
     3361 cgcgcagagc gcgctgactg tttccgaaac taccttcggt cgtgatttca acgaagcgct
     3421 ggttcaccag gttgttgttg cttatgcagc tggtgctcgt cagggtactc gtgctcagaa
     3481 gactcgtgct gaagtaactg gttccggtaa aaaaccgtgg cgccagaaag gcaccggccg
     3541 tgcgcgttct ggttctatca agagcccgat ctggcgttct ggtggcgtga cctttgctgc
     3601 tcgtccgcag gaccacagtc aaaaagttaa caagaagatg taccgcggcg cgctgaaaag
     3661 catcctgtcc gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt tctctgtaga
     3721 agcgccgaaa actaagctgc tggcacagaa actgaaagac atggctctgg aagatgtgct
     3781 gatcatcacc ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc tgcacaaggt
     3841 tgacgtacgc gatgcaactg gtatcgaccc ggttagcctg atcgccttcg acaaagtcgt
     3901 aatgactgct gatgctgtta agcaagttga ggagatgctg gcatgattcg tgaagaacgt
     3961 ctgctgaagg tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc gatggaaaaa
     4021 tccaacacca tcgtactcaa agttgctaaa gacgcgacca aagcagaaat caaagctgct
     4081 gtgcagaaac tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt taaagggaaa
     4141 gttaaacgtc acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc ttacgtcacc
     4201 ctgaaagaag gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg aggagtaata
     4261 caatggcagt tgttaaatgt aaaccgacat ctccgggtcg tcgccacgta gttaaagtgg
     4321 ttaaccctga gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa aacagcaaat
     4381 ccggtggtcg taacaacaat ggccgtatca ccactcgtca tatcggtggt ggccacaagc
     4441 aggcttaccg tattgttgac ttcaaacgca acaaagacgg tatcccggca gttgttgaac
     4501 gtcttgagta cgatccgaac cgttccgcga acatcgcgct ggttctgtac aaagacggtg
     4561 aacgccgtta catcctggcc cctaaaggcc tgaaagctgg cgaccagatt cagtctggcg
     4621 ttgatgctgc aatcaaacca ggtaacaccc tgccgatgcg caacatcccg gttggttcta
     4681 ctgttcataa cgtagaaatg aaaccaggta aaggcggtca gctggcacgt tccgctggta
     4741 cttacgttca gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg cgttctggtg
     4801 aaatgcgtaa agtagaagca gactgccgtg caactctggg cgaagttggc aatgctgagc
     4861 atatgctgcg cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt cgtccgaccg
     4921 ttcgcggtac cgcgatgaac ccggtagacc acccacatgg tggtggtgaa ggtcgtaact
     4981 ttggtaagca cccggtaact ccgtggggcg ttcagaccaa aggtaagaag acccgcagca
     5041 acaagcgtac tgataaattc atcgtacgtc gccgtagcaa ataattttag aggataagcc
     5101 atgccacgtt ctctcaagaa aggtcctttt attgacctgc acttgctgaa gaaggtagag
     5161 aaagcggtgg aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg ttcaacgatc
     5221 tttcctaaca tgatcggttt gaccatcgct gtccataatg gtcgtcagca cgttccggta
     5281 tttgtaaccg acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac tcgtacttat
     5341 cgcggccacg ctgctgataa aaaagcgaag aagaaataag gtaggaggaa gagatggaaa
     5401 ctatcgctaa acatcgccat gctcgttctt ctgctcagaa ggttcgcctt gttgctgacc
     5461 tgattcgcgg taagaaagtg tcgcaggctc tggatatttt gacctacacc aacaagaaag
     5521 cggctgtact ggtcaagaaa gttctggaat ctgccattgc taacgctgaa cacaacgatg
     5581 gcgctgacat tgacgatctg aaagttacga aaattttcgt agacgaaggc ccgagcatga
     5641 agcgcattat gccgcgtgca aaaggtcgtg cagatcgcat cctgaagcgc accagccaca
     5701 tcactgtggt tgtgtccgat cgctgagact ctggagacta gcaatgggtc agaaagtaca
     5761 tcctaatggt attcgcctgg gtattgtaaa accatggaac tctacctggt ttgcgaacac
     5821 caaagaattc gctgacaacc tggacagcga ttttaaagta cgtcagtacc tgactaagga
     5881 actggctaaa gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga gcatccgtgt
     5941 aaccattcac actgctcgcc cgggtatcgt tatcggtaaa aaaggtgaag acgtagaaaa
     6001 actgcgtaag gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca tcgccgaagt
     6061 tcgtaagcct gaactggacg caaaactggt tgctgacagc atcacttctc agctggaacg
     6121 tcgcgttatg ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc gtctgggcgc
     6181 taaaggtatt aaagttgaag ttagcggccg tctgggcggc gcggaaatcg cacgtaccga
     6241 atggtaccgc gaaggtcgcg taccgctgca cactctgcgt gctgacatcg actacaacac
     6301 ctctgaagcg cacaccactt acggtgtaat cggcgttaaa gtgtggatct tcaaaggcga
     6361 gatcctgggt ggtatggctg ctgttgaaca accggaaaaa ccggctgctc agcctaaaaa
     6421 gcagcagcgt aaaggccgta aataaggagc gtcgctgatg ttacaaccaa agcgtacaaa
     6481 attccgtaaa atgcacaaag gccgtaaccg cggtctggcg cagggtacgg atgttagctt
     6541 cggcagcttc ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc agatcgaagc
     6601 agcacgtcgt gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga tccgtgtgtt
     6661 cccggacaaa ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag gtaaaggtaa
     6721 cgtggagtat tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa tggacggtgt
     6781 tccggaagag ctggcccgtg aagcattcaa gctggcagca gcgaaactgc cgattaaaac
     6841 cacctttgta actaagacgg tgatgtaatg aaagcaaaag agctgcgtga gaagagcgtt
     6901 gaagagctga acaccgagct gctgaacctg ctgcgtgagc agttcaacct gcgtatgcag
     6961 gctgcaagtg gccagctgca acagtctcac ctgttgaagc aagtgcgtcg cgatgtcgca
     7021 cgcgttaaga ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa atccgtactc
     7081 tgcaaggtcg cgttgttagc gacaaaatgg agaaatccat tgttgttgct atcgaacgtt
     7141 ttgtgaaaca cccgatctac ggtaaattca tcaagcgtac gaccaaactg cacgtacatg
     7201 acgagaacaa cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc cgtccgctgt
     7261 ccaagactaa atcctggacg ctggttcgcg ttgtagagaa agcggttctg taatacagta
     7321 cactctctcg atacgaataa acggctcaga aatgagccgt ttattttttc tacccatatc
     7381 cttgaagcgg tgttataatg ccgcgccctc gatatgggga tttttaacga cctgattttc
     7441 gggtctcagt agtagttgac attagcggag cactaaaatg atccaagaac agactatgct
     7501 gaacgtcgcc gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc tgggtggctc
     7561 gcaccgtcgc tacgcaggcg taggcgacat catcaagatc accatcaaag aagcaattcc
     7621 gcgtggtaag gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca ccaagaaggg
     7681 tgttcgtcgc ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg ttcttctgaa
     7741 caacaacagc gagcagccta tcggtacgcg tatttttggg ccggtaactc gtgagcttcg
     7801 tagtgagaag ttcatgaaaa ttatctctct ggcaccagaa gtactctaag gagcgaatca
     7861 tggcagcgaa aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa gataaaggta
     7921 aacgcggtaa agttaagaat gtcctgtctt ccggcaaggt cattgttgaa ggtatcaacc
     7981 tggttaagaa acatcagaag ccggttccgg ccctgaacca accgggtggc atcgttgaaa
     8041 aagaagccgc tattcaggtt tccaacgtag caatcttcaa tgcggcaacc ggcaaggctg
     8101 accgtgtagg ctttagattc gaagacggca aaaaagtccg tttcttcaag tctaacagcg
     8161 aaactatcaa gtaatttgga gtagtacgat ggcgaaactg catgattact acaaagacga
     8221 agtagttaaa aaactcatga ctgagtttaa ctacaattct gtcatgcaag tccctcgggt
     8281 cgagaagatc accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa aactgctgga
     8341 taacgcagca gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca ccaaagcacg
     8401 caaatctgtt gcaggcttca aaatccgtca gggctatccg atcggctgta aagtaactct
     8461 gcgtggcgaa cgcatgtggg agttctttga gcgcctgatc actattgctg tacctcgtat
     8521 ccgtgacttc cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact acagcatggg
     8581 tgtccgtgag cagatcatct tcccagaaat cgactacgat aaagtcgacc gcgttcgtgg
     8641 tttggatatt accattacca ctactgcgaa atctgacgaa gaaggccgcg ctctgctggc
     8701 tgcctttgac ttcccgttcc gcaagtaagg tagggttact aaatggctaa gcaatcaatg
     8761 aaagcacgcg aagtaaaacg cgtagcttta gctgataaat acttcgcgaa acgcgctgaa
     8821 ctgaaagcga tcatctctga tgtgaacgct tccgacgaag atcgttggaa cgctgttctc
     8881 aagctgcaga ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg ctgccgtcaa
     8941 acaggtcgtc cgcatggttt cctgcggaag ttcgggttga gccgtattaa ggtccgtgaa
     9001 gccgctatgc gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt gtcaccaatt
     9061 gaatcacggg agtaaagaca gatgagcatg caagatccga tcgcggatat gctgacccgt
     9121 atccgtaacg gtcaggccgc gaacaaagct gcggtcacca tgccttcctc caagctgaaa
     9181 gtggcaatcg ccaacgtgct gaaggaagaa ggttttattg aagattttaa agttgaaggc
     9241 gacaccaagc ctgaactgga acttactctg aagtatttcc agggcaaagc tgttgtagaa
     9301 agcattcagc gtgtcagccg cccaggtctg cgcatctata aacgtaaaga tgagctgccg
     9361 aaagttatgg cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt tatgactgat
     9421 cgtgcagcgc gccaggctgg tcttggtggc gaaattatct gctacgtagc ctaatcggag
     9481 gaaaaaatgt ctcgtgttgc taaagcaccg gtcgttgttc ctgccggcgt tgacgtaaaa
     9541 atcaacggtc aggttattac gatcaaaggt aaaaacggcg agctgactcg tactctcaac
     9601 gatgctgttg aagttaaaca tgcagataat accctgacct tcggtccgcg tgatggttac
     9661 gcagacggtt gggcacaggc tggtaccgcg cgtgccctgc tgaactcaat ggttatcggt
     9721 gttaccgaag gcttcactaa gaagctgcag ctggttggtg taggttaccg tgcagcggtt
     9781 aaaggcaatg tgattaacct gtctctgggt ttctctcatc ctgttgacca tcagctgcct
     9841 gcgggtatca ctgctgaatg tccgactcag actgaaatcg tgctgaaagg cgctgataag
     9901 caggtgatcg gccaggttgc agcggatctg cgcgcctacc gtcgtcctga gccttataaa
     9961 ggcaagggtg ttcgttacgc cgacgaagtc gtgcgtacca aagaggctaa gaagaagtaa
    10021 ggtaacacta tggataagaa atctgctcgt atccgtcgtg cgacccgcgc acgccgcaag
    10081 ctccaggagc tgggcgcaac tcgcctggtg gtacatcgta ccccgcgtca catttacgca
    10141 caggtaattg caccgaacgg ttctgaagtt ctggtagctg cttctactgt agaaaaagct
    10201 atcgctgaac aactgaagta caccggtaac aaagacgcgg ctgcagctgt gggtaaagct
    10261 gtcgctgaac gcgctctgga aaaaggcatc aaagatgtat cctttgaccg ttccgggttc
    10321 caatatcatg gtcgtgtcca ggcactggca gatgctgccc gtgaagctgg ccttcagttc
    10381 taaggtagag gtgtaagatg gctcacatcg aaaaacaagc tggcgaactg caggaaaagc
    10441 tgatcgcggt aaaccgcgta tctaaaaccg ttaaaggtgg tcgtattttc tccttcacag
    10501 ctctgactgt agttggcgat ggtaacggtc gcgttggttt tggttacggt aaagcgcgtg
    10561 aagttccagc agcgatccag aaagcgatgg aaaaagcccg tcgcaatatg attaacgtcg
    10621 cgctgaataa cggcactctg caacaccctg ttaaaggtgt tcacacgggt tctcgcgtat
    10681 tcatgcagcc ggcttccgaa ggtaccggta tcatcgccgg tggtgcaatg cgcgccgttc
    10741 tggaagtcgc tggggttcat aacgttctgg ctaaagcata tggttccacc aacccgatca
    10801 acgtggttcg tgcaactatt gatggcctgg aaaatatgaa ttctccagaa atggtcgctg
    10861 ccaagcgtgg taaatccgtt gaagaaattc tggggaaata aaccatggca aagactatta
    10921 aaattactca aacccgcagt gcaatcggtc gtctgccgaa acacaaggca acgctgcttg
    10981 gcctgggtct gcgtcgtatt ggtcacaccg tagagcgcga ggatactcct gctattcgcg
    11041 gtatgatcaa cgcggtttcc ttcatggtta aagttgagga gtaagagatg cgtttaaata
    11101 ctctgtctcc ggccgaaggc tccaaaaagg cgggtaaacg cctgggtcgt ggtatcggtt
    11161 ctggcctcgg taaaaccggt ggtcgtggtc acaaaggtca gaaatctcgt tctggcggtg
    11221 gcgtacgtcg cggtttcgag ggtggtcaga tgcctctgta ccgtcgtctg ccgaaattcg
    11281 gcttcacttc tcgtaaagca gcgattacag ccgaagttcg tctgtctgac ctggctaaag
    11341 tagaaggcgg tgttgtagac ctgaacacgc tgaaagcggc taacattatc ggtatccaga
    11401 tcgagttcgc gaaagtgatc ctggctggcg aagtaacgac tccggtaact gttcgtggcc
    11461 tgcgtgttac taaaggcgct cgtgctgcta tcgaagctgc tggcggtaaa atcgaggaat
    11521 aagtagcaga tggctaaaca accgggatta gattttcaaa gtgccaaagg tggcttaggc
    11581 gagctgaaac gcagactgct gtttgttatc ggtgcgctga ttgtgttccg tattggctct
    11641 tttattccga tccctggtat tgatgccgct gtacttgcca aactgcttga gcaacagcga
    11701 ggcaccatca ttgagatgtt taacatgttc tctggtggtg ctctcagccg tgcttctatc
    11761 tttgctctgg ggatcatgcc gtatatttcg gcgtcgatca ttatccagct gctgacggtg
    11821 gttcacccaa cgttggcaga aattaagaaa gaaggggagt ctggtcgtcg taagatcagc
    11881 cagtacaccc gttacggtac tctggtgctg gcaatattcc agtcgatcgg tattgctacc
    11941 ggtctgccga atatgcctgg tatgcaaggc ctggtgatta acccaggctt tgcattctac
    12001 ttcaccgctg ttgtaagtct ggtcacagga acgatgttcc tgatgtggtt gggcgaacag
    12061 attactgaac gaggtatcgg caacggtatt tcaatcatta tcttcgccgg tattgtcgcg
    12121 ggactcccgc cagccattgc ccatactatc gagcaagcgc gtcaaggcga cctgcacttc
    12181 ctcgtgttgc tgttggttgc agtattagta tttgcagtga cgttctttgt tgtatttgtt
    12241 gagcgtggtc aacgccgcat tgtggtaaac tacgcgaaac gtcagcaagg tcgtcgtgtc
    12301 tatgctgcac agagcacaca tttaccgctg aaagtgaata tggcgggggt aatcccggca
    12361 atcttcgctt ccagtattat tctgttcccg gcgaccatcg cgtcatggtt cgggggcggt
    12421 actggttgga actggctgac aacaatttcg ctgtatttgc agcctgggca accgctttat
    12481 gtgttactct atgcgtctgc aatcatcttc ttctgtttct tctacacggc gttggttttc
    12541 aacccgcgtg aaacagcaga taacctgaag aagtccggtg catttgtacc aggaattcgt
    12601 ccgggagagc aaacggcgaa gtatatcgat aaagtaatga cccgcctgac cctggttggt
    12661 gcgctgtata ttacctttat ctgcctgatc ccggagttca tgcgtgatgc aatgaaagta
    12721 ccgttctact tcggtgggac ctcactgctt atcgttgttg tcgtgattat ggactttatg
    12781 gctcaagtgc aaactctgat gatgtccagt cagtatgagt ctgcattgaa gaaggcgaac
    12841 ctgaaaggct acggccgata attggtcgcc cgagaagtta cggagagtaa aaatgaaagt
    12901 tcgtgcttcc gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg atggtgtcat
    12961 ccgtgtgatt tgcagtgccg agccgaagca taaacagcgc caaggctgat tttttcgcat
    13021 atttttcttg caaagttggg ttgagctggc tagattagcc agccaatctt ttgtatgtct
    13081 gtgcgtttcc atttgagtat cctgaaaacg ggcttttcag catggaacgt acatattaaa
    13141 tagtaggagt gcatagtggc ccgtatagca ggcattaaca ttcctgatca taagcatgcc
    13201 gtaatcgcat taacttcgat ttatggcgtc ggcaagaccc gttctaaagc catcctggct
    13261 gcagcgggta tcgctgaaga tgttaagatc agtgagctgt ctgaaggaca aatcgacacg
    13321 ctgcgtgacg aagttgccaa atttgtcgtt gaaggtgatc tgcgccgtga aatcagcatg
    13381 agcatcaagc gcctgatgga tcttggttgc tatcgcggtt tgcgtcatcg tcgtggtctc
    13441 ccggttcgcg gtcagcgtac caagaccaac gcacgtaccc gtaagggtcc gcgcaaaccg
    13501 atcaagaaat aatcggggtg attgaataat ggcaaaggca ccaattcgtg cacgtaaacg
    13561 tgtaagaaaa caagtctctg acggcgtggc tcatatccat gcttctttca acaacaccat
    13621 cgtgactatc actgatcgtc agggtaacgc gttgggttgg gcaacagccg gtggttccgg
    13681 tttccgtggt tctcgcaaat ccactccgtt tgcagctcag gttgcagcag agcgttgcgc
    13741 tgacgccgtg aaagaatacg gcatcaagaa tctggaagtt atggttaaag gtccgggtcc
    13801 aggccgcgaa tctactattc gtgctctgaa cgccgcaggt ttccgcatca ctaatattac
    13861 tgatgtgact ccgatccctc ataacggttg tcgtccgccg aaaaaacgtc gcgtataacg
    13921 cctcgttttc caggtttgtt ggagaaagaa aatggcaaga tatttgggtc ctaagctcaa
    13981 gctgagccgt cgtgagggca ccgacttatt ccttaagtct ggcgttcgcg cgatcgatac
    14041 caagtgtaaa attgaacaag ctcctggcca gcacggtgcg cgtaaaccgc gtctgtctga
    14101 ctatggtgtg cagttgcgtg aaaagcaaaa agttcgccgt atctatggtg tgctggagcg
    14161 tcagttccgt aactactaca aagaagcagc acgtctgaaa ggcaacaccg gtgaaaacct
    14221 gttggctctg ctggaaggtc gtctggacaa cgttgtatac cgtatgggct tcggtgccac
    14281 tcgtgcagaa gcacgtcagc tggttagcca taaagcaatt atggtaaacg gtcgtgttgt
    14341 taacatcgct tcttatcagg ttagtccgaa tgacgttgta agcattcgtg agaaagcgaa
    14401 gaagcagtct cgcgtgaaag ccgctctgga gctggctgag cagcgtgaaa agccaacctg
    14461 gctggaagtt gatgctggca agatggaagg tacgtttaag cgtaagccgg agcgttctga
    14521 tctgtctgcg gacattaacg aacacctgat cgtcgagctt tactccaagt aaagcttagt
    14581 accaaagaga ggacacaatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg
    14641 atatcgagca agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct
    14701 ttggccatac tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg
    14761 cggtgaccga ggttgagatt gatggtgtac tacatgagta cagcaccaaa gaaggcgttc
    14821 aggaagatat cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca
    14881 aagatgaagt tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata
    14941 tcacccacga cggtgatgtc gaaatcgtca agccgcagca cgtgatctgc cacctgaccg
    15001 atgagaacgc gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg
    15061 cttctacccg aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg
    15121 catgctacag ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc
    15181 gtaccgacct ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag
    15241 aggcgattcg tcgtgcggca accattctgg ctgaacaact ggaagctttc gttgacttac
    15301 gtgatgtacg tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg atcctgctgc
    15361 gccctgttga cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa gcagaagcta
    15421 tccactatat cggtgatctg gtacagcgta ccgaggttga gctccttaaa acgcctaacc
    15481 ttggtaaaaa atctcttact gagattaaag acgtgctggc ttcccgtgga ctgtctctgg
    15541 gcatgcgcct ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg gatcacaggt
    15601 taaggtttta ctgagaagga taaggtcatg cgccatcgta agagtggtcg tcaactgaac
    15661 cgcaacagca gccatcgcca ggctatgttc cgcaatatgg caggttcact ggttcgtcat
    15721 gaaatcatca agacgactct gcctaaagcg aaagagctgc gccgcgtagt tgagccgctg
    15781 attactcttg ccaagactga tagcgttgct aatcgtcgtc tggcattcgc ccgtactcgt
    15841 gataacgaga tcgtggcaaa actgtttaac gaactgggcc cgcgtttcgc gagccgtgcc
    15901 ggtggttaca ctcgtattct gaagtgtggc ttccgtgcag gcgacaacgc gccgatggct
    15961 tacatcgagc tggttgatcg ttcagagaaa gcagaagctg ctgcagagta atctgaagca
    16021 acgtaaaaaa acccgccccg gcgggttttt ttatacccgt agcatcccca cttatctaca
    16081 atagctgtac tctttttgtt catcccctgg agtatatatg tggttacttg accagtgggc
    16141 agagcgtcat atagcagaag cgcaagcgaa aggtgagttt gataacctgg caggtagcgg
    16201 cgaaccattg atactggatg atgattctca cgtgccaccg gaattacgtg cggggtatcg
    16261 cttgttgaag aatgccggtt gcttaccgcc agaacttgag caacggaggg aagcaattca
    16321 gcttctggat attctcaaag gtatccgtca cgatgatccg caatatcaag aggttagccg
    16381 tcgattgtca ttactggaat tgaagctgcg acaagctgga ctgagtaccg attttttacg
    16441 cggcgattat gctgacaagt tgttgaataa aatcaacgat aactagtgga gtatgtatgt
    16501 atcgcattgg tgagctggca aaaatggcgg aagtaacacc cgacacgatt cgttattacg
    16561 aaaaacagca gatgatggag catgaagtgc gtactgaagg tgggtttcgc ctgtataccg
    16621 aaagcgatct ccagcgattg aaatttatcc gccatgccag acaactaggt ttcagtctgg
    16681 agtcgatccg cgagttgctg tcgatccgta tcgatcctga acaccatacc tgtcaggagt
    16741 caaaaggcat tgtgcaggaa agattgcagg aagtcgaagc acggatagcc gagttgcaga
    16801 gtatgcagcg ttccttgcaa cgccttaacg atgcctgttg tgggaccgcc catagcagtg
    16861 tttattgctc gattcttgaa gctcttgaac aaggggcgag tggcgttaag agtggctgtt
    16921 gattttttgc actgacagga ttacactcgc gccgttaaat aaccaactgg agtttttatg
    16981 agccgatatc agcatactaa agggcagata aaggataatg cgatagaagc attactacat
    17041 gatcccttat tccgacagcg agtagagaaa aataagaaag ggaaaggcag ttacatgcga
    17101 aaaggaaaac atggcaatcg ggggaactgg gaggccagtg gcaagaaagt aaatcacttt
    17161 tttaccactg gccttctgct ttcaggcgtt tgttaagagc ggttattctg ctctttcagc
    17221 aaatcacgaa tttctgtcag taatacttct tctttagttg gtgctggtgc ggctgctggt
    17281 tcttcttttt tccgattcag tttgttgatt agcttaatcg ccataaagat ggcaaaggcc
    17341 acaatcagaa aatcaaagac gttttgaatg aagacaccgt aatgcatcac aacagcaggg
    17401 atatccccct gcgcatcgcg tagcgtgaca gcaaactgtt taaagtcgat cccgccaatt
    17461 aataagccca gaggtggcat gatgatatcg gcaaccagtg aagatacaat cttcccgaat
    17521 gccgcaccga taatgacacc caccgccaaa tccaccacgt tcccgcgcat cgcaaattcg
    17581 cgaaattctt taataatgct catgttattc tccctatgaa gccgacaacc ataagtctaa
    17641 caaatgttaa gccattttcc tgccaggaat gaatttaatt aatccttgat aatcaaaggg
    17701 aaataataag gcgtcattag acgccttatt aattacaaga agaaagggct tggctggaag
    17761 agtctttcga cgtcggtaat aaacttttta tctgtgagga acataattac gtgatcgcct
    17821 tgctcaatgc gcagattgtc attggcaatc atcacgtcgt ttccacgtac caccgctcca
    17881 ataatcgttc ctggcggtag cttgatttcg tcaatgactc tgccgacaac gcgtgaggtg
    17941 ctttcatcac cgtgagcaac ggcttcaata gcttctgcta cgccgcggcg caatgaggaa
    18001 acaccaacaa tatctgcttt tcgcacatgg ctaagcaacg cagaaatagt tgcttgttgt
    18061 ggtgaaatcg caatatcgat cacgctcccc tgaaccagat ccacataagc gcgacgctgg
    18121 atcaatacca tcaccttttt cgcacccata cgtttggcaa gcatggcgga catgatattg
    18181 gcctcgtcat cgttggtgac ggcaataaac agatcaactt gatcgatatg ttcttcggcc
    18241 agcagttctt gatccgacgc atcaccaaaa aagacgatcg tattctgcaa cttttccgcc
    18301 agttcggcag cgcgctgctg attacgttcg atgagtttaa cgctgtaatc tttttccaga
    18361 cgacgcgcca gccctgcacc gatattaccg ccaccaacca gcatgatccg cttatacggt
    18421 ttttccagtc gctgtaattc actcatcacc gcacggatat gctgtgaagc ggcaataaag
    18481 aacacttcat caccagcttc cacaatggtc gaaccttgtg gacgaatggg gcgatcgtgg
    18541 cggaaaattg ctgccacacg agtatcgata tgtggcatat gttcgcgcat ggtcgaaagt
    18601 gcgttaccaa tcagcgggcc gccataatag gctttaacca cagcgaggct gactttaccc
    18661 tcagcgaagt tcaccacctg caatgcgccg ggatactcaa tcagtcggta aatattatcg
    18721 ataaccaact gctctggtgc gatcagatga tcaatcggta cagcatctga atgaaatagc
    18781 ttatcggcat cgcgcacgta gtctggtgag cggatgcgag cgatgcgatt aggggtgttg
    18841 aaaagtgagt aggctacctg gcaggcaacc atattggttt catctgaact ggttacagca
    18901 accagcatat cggcgtcgtc ggcacctgcc tcccgcaata cgcgtggatg agagccatgc
    18961 ccctgcacga ctcgcaggtc aaatttatcc tgcaaggtcc gcagacgctc accgttggta
    19021 tcgacaacag tgatatcgtt gttctcgcca accaggtttt ccgccagtgt gccgccaacc
    19081 tggccggcac ccagaatgat aattttcatc agtcgcgacc cgttatctca tcactttttg
    19141 attagcttag cgtaaaagaa gccgtcgccc tcttcggcac caggcagatt ttgtttaccc
    19201 ggttgctctg gtgttcctgt ttcgcaaagt tcggcatcag cggtacgttg caaaaaggct
    19261 ttaatctgca ggctattctc ttccggtaac accgaacagg tggcatagac cagggttcca
    19321 ccggatttta aatgcggcca aatggcgtcg agaatttcag actgcaattg cgcgagttcc
    19381 gggatatcgc gatcgcgacg taaccattta atatccggat ggcgacgaat cacaccggtt
    19441 gctgaacaag gcgcatctaa taaaatgcga tcaaactgtt gctcgccaca ccattgggaa
    19501 gggtagcggc catcaccttg tttcacggtc gctttcatac caaggcgttt taaattgtcg
    19561 taaacgcgag agaggcgctg ttcgtcaata tcaaccgcaa caacctgcgc ttctggtgcc
    19621 acctcaagga tatgcgttgt tttaccgccg ggggcggcac aaagatccag aatgtgttca
    19681 ccgttttgtg gcgcaagcca ggtcatgcaa ccttgtgctg atgcatcctg aacggtaacc
    19741 catccgtctt caaaaccagg tagcgcatga acaggtgcag gtgtttccag ccgtacagca
    19801 tcagggtaat ccgcatgcgg gaaacctttc attcctgctt catccaacaa tgcaagccag
    19861 ctgtcgcggg aatgatgcgt acgattaacg cgcagccaca ttggcggacg ctggttattg
    19921 gcttcgacga tggattgcca ctgctctgga tacgcttttt gcagacgctt cagcaaccag
    19981 gaaggatgca gataacgtgc atcactggca ttaaactcgg ctaataactc ttcttgctga
    20041 cgctggaact ggcgtaatac accgtttatc aaccctttaa gttgcggacg cttaattgcg
    20101 atagcgcctt caaccgtttc agccagcgca gcatgaggtg gaatgcgggt ataaagcagt
    20161 tgatacaaac caaccataat caggtaatgc acggtccgct gtttgccggt catcggacgg
    20221 gccattaact tattaatcag ccagtctaac tgcgaaagcg tacgcagtac gccaaagcac
    20281 aactcttgaa gaagtgcttt gtctttatcg gaaacttttt gctggagcgg tggcagaatg
    20341 ttgcttaatg attgcccttg ctcgacgact tgttcaacgg cctgggccgc catgctacgt
    20401 aaattacgtt gttttttcat aggtataaaa atgcccggca agaccgggct tagaagagtg
    20461 gactatcaga ccagacggtt gcccggaaca aaccattccc gacgagagtt caggaggtct
    20521 tgcgcgctca tcgctttctt acccgcaggt tgtaacgaga gcaggttcag gatgccatca
    20581 ccagtcgcaa cctgaatgcc ttgtttattg gcttcaagga tcgttcctgg tgcagcgttg
    20641 gttgccgtat caatgaccga cgctttccag actttaaccg gttgtccttc aatttccagc
    20701 cagctcattg gccatgggtt gaaagcgcga atgcagcgtt caagctgcgc tgccgaaagc
    20761 gaccagtcaa tacgcgcttc ttctttactc aacttctcgg cgtaagtgac aagagtttcg
    20821 tcctgaactt ctggtttcgc cgtgccgtct gccagttgtt tcaacgtggt gataagccct
    20881 tgtgggccaa gctctgccag cttgtcgtac agcgtaccac tggtatcttc tgcagtaatc
    20941 gggcaggaga gcttatagag catgtcaccg gtgtctaaac caacatccat ttgcataatg
    21001 gtcacaccag tttctgcatc acccgcccat agtgagcgtt ggattggtgc agcaccgcgc
    21061 cagcgtggca gcagtgaacc atgaacgttg atacagccaa gacgcggcat ctccagcaca
    21121 gctttaggca gaattaaacc ataggcgacg acgaccataa catcagcctg cagatcggcg
    21181 accagttgct ggttttcttg tggacgcagg gaaacaggtt gaaaaacggg cagacctttt
    21241 tcctcagcca gaactttaat cgggctgggc atcagttttt taccgcgtcc tgccggtcgg
    21301 tctggctggg tgaacacgcc aacgacgtta tgaccagaag acaacagcgc gtcgagatga
    21361 cgcgctgcaa agtcaggtgt acccgcaaaa ataatacgta gtgattctga cacgttagtt
    21421 cttatcctta agcccgggct ttcagacgat ccagtttttc aactttctga cgaatacgtt
    21481 gttgtttcag cggtgacaga taatccataa acagtttgcc gaccaggtga tccatctcat
    21541 gctgaataca gatggctaac agaccatctg cctccagttc aaatggttta ccgtcgcggt
    21601 ccagggcgcg aattttaact ttctctgcgc gtggcactaa agcacgttgt tcagggatcg
    21661 acaggcaacc ttcttcaatg cctgtttcgc cgcttttttc taaaagctcc ggattgatta
    21721 acaccagccg ttcgtcacgg ttttccgaaa catcaataac aatgatacgt tgatggatat
    21781 caacctgggt tgccgccagg ccaatacctt cttctgcata catcgtctcg aacatatcat
    21841 cgacgatacg ctgaatttct gcattcactt cttctaccgg tttagcaact ttgcgaagcc
    21901 gctcgtccgg aatatgtaac acttgcaaaa ctgacataaa tctccagaga tgtgttcagg
    21961 agttagaaag attatttctt ctattctaga caaatccccc tctgattgac agcatcactg
    22021 accaatcgca aagattgcta aggctgctta tggcagggag ataaggatgg tcgatacaga
    22081 aatttggctg cgtttaatga gtatcagcag cttgtacggc gatgatatgg tccgtatagc
    22141 tcactggctg gcaaaacagt cgcatattga tgcggttgta ttgcagcaaa ctgggcttac
    22201 attgcggcag gcacaacgct ttctttcatt tccgcgaaag agtatcgaaa gctcactttg
    22261 ttggttggag caacccaacc atcatttaat ccctgcggac agcgaatttt atcctcctca
    22321 acttcaggcg acgacagatt accccggcgc actgtttgtt gaaggagaac tgcacgcgct
    22381 gcattcattt cagcttgcca tagtggggag tcgggcgcat tcatggtatg gcgagcgatg
    22441 gggacgatta ttttgcgaaa ctctggcgac gcgtggagtg acaattacaa gtggactggc
    22501 gcgtggaatc gatggtgtgg cgcataaagc ggccttacag gtaaatggcg tcagcattgc
    22561 tgtattgggg aatggactta ataccattca tccccgccgc catgcccgac tggctgccag
    22621 tttgcttgaa catggcggag ctctcgtctc ggaatttccc ctcgatgttc caccccttgc
    22681 ttacaatttc ccacgaagaa atcgcattat cagtggtcta agtaaaggtg tactggtggt
    22741 ggaagcggct ttgcgcagtg gttcgctagt gacagcacgt tgtgcgcttg agcaggggcg
    22801 tgaagttttt gccttgccag gtccaatagg gaatccggga agcgaagggc ctcactggtt
    22861 aataaaacaa ggtgcgattc ttgtgacgga accggaagaa attctggaaa acttgcaatt
    22921 tggattgcac tggttgccag acgcccctga aaattcattt tattcaccag atcagcaaga
    22981 cgtggcattg ccatttcctg agctcctggc taacgtagga gatgaggtaa cacctgttga
    23041 cgtcgtcgct gaacgtgccg gccaacctgt gccagaggta gttactcaac tactcgaact
    23101 ggagttagca ggatggatcg cagctgtacc cggcggctat gtccgattga ggagggcatg
    23161 ccatgttcga cgtactaatg tatttgtttg aaacctatat tcacacagaa gctgagttgc
    23221 gtgtggatca agacaaactt gaacaggatc ttaccgacgc aggatttgat cgagaagata
    23281 tctacaatgc cctgctttgg ctggaaaagc ttgcggatta tcaggaagga ctggcagaac
    23341 cgatgcaact ggcctctgat ccactctcca tgcgtattta tacccctgag gagtgtgaaa
    23401 gactggatgc cagctgtcgt ggttttctgc ttttccttga gcagattcag gtgctcaacc
    23461 ttgaaactcg tgaaatggtg atagagcgag tgctcgcgct ggataacgca gagttcgaac
    23521 tggatgatct gaaatgggta atcctgatgg tgttgttcaa tattccgggc tgcgaaaatg
    23581 cgtaccagca aatggaagaa ttactctttg aagtgaatga aggtatgctg cattaatcat
    23641 ttctttaatt cagcataagt tgttatggcg aaatcagcac tgttcacggt gcgtaataat
    23701 gagtcctgcc caaagtgcgg ggctgaactg gttattcgat ccgggaaaca cggtccgttt
    23761 cttggatgct cacagtatcc ggcgtgtgac tacgtccgtc ctctgaaatc ttcagcggat
    23821 ggacatattg tcaaagttct ggaggggcag gtttgccctg catgtggcgc aaatctggta
    23881 ttacgccagg gacgctttgg tatgtttatt ggttgcagta actaccctga atgcgaacat
    23941 accgaactta tcgataaacc ggacgaaaca gcaattacat gcccccaatg ccggacgggc
    24001 catctggtcc agcgccgctc ccgttatggc aaaacatttc actcctgtga tcgctacccg
    24061 gagtgtcaat ttgccattaa cttcaaaccc atagctggag aatgccctga gtgtcattat
    24121 ccactactca tcgaaaagaa aaccgcgcag ggtgtaaaac acttttgtgc cagtaaacaa
    24181 tgtggaaagc cggtttcggc ggaataataa cgtgaataat aacctgcaag gagacgctat
    24241 cgcagctgcg atagatgtcc tcaatgaaga acgtgtcatc gcctatccaa cggaagccgt
    24301 tttcggtgtc gggtgcgatc ctgatagcga aacagcagtg atgcgactgt tggagttaaa
    24361 acagcgtccg gttgataagg ggctgatttt aatcgcggca aattacgagc agcttaaacc
    24421 ctatattgat gacaccatgc tgactgatgc gcagcgtgaa accatttttt cccgctggcc
    24481 aggtcctgtc acctttgtct ttcccgcgcc tgcgacaaca ccgcgctggt tgacgggccg
    24541 ctttgattcg cttgctgtac gagtcaccga ccatccgttg gtggttgctt tgtgccaggc
    24601 ttatggtaaa ccgctggttt ctaccagtgc caacttgagt ggattgccac cttgtcgaac
    24661 agtagacgaa gttcgcgcac aatttggcgc ggcgttcccg gttgtgcctg gtgaaacggg
    24721 ggggcgttta aatccttcag aaatccgcga tgccctgacg ggtgaactgt ttcgacaggg
    24781 gtaacataat ggaaacctat gctgtttttg gtaatccgat agcccacagc aaatcgccat
    24841 tcattcatca gcaatttgct caacaactga atattgaaca tccctatggg cgcgtgttgg
    24901 cacccatcaa tgatttcatc aacacactga acgctttctt tagtgctggt ggtaaaggtg
    24961 cgaatgtgac ggtacctttt aaagaagagg cttttgccag agcggatgag cttactgaac
    25021 gggcagcgtt ggctggtgct gttaataccc tcaagcggtt agaagatgga cgcttgcagg
    25081 gtgacaatac cgatggtata ggtttgttaa gcgatctgga acgtctgtct tttatccgtc
    25141 cgggtttacg tattctgctt atcggcgctg gtggagcatc tcgcggcgta ctactaccac
    25201 tcctttccct ggactgtgcg gtgacaatca ctaatcggac ggtatcccgc gcggaagagt
    25261 tggctaaatt gtttgcgcac actggcagta ttcaggcgtt gggtatggac gaactggaag
    25321 gtcatgagtt tgatctcatt attaatgcaa catccagtgg catcagtggt gatattccgg
    25381 cgatcccgtc atctctcatt catccaggca tttattgcta tgacatgttc tatcagaaag
    25441 gaaaaactcc ttttctggca tggtgtgagc agcgaggctc aaagcgtaat gctgatggtt
    25501 taggaatgct ggtggcacag gcggctcatg cctttcttct ctggcacggt gttctgcctg
    25561 acgtagaacc agttataaag caattgcagg aggaattgtc cgcgtgaatc aggccatcca
    25621 gtttccggac agagaagagt gggacgagaa taaaaaatgt gtatgttttc ccgctctcgt
    25681 gaatggcatg caactgacat gtgcgatctc tggcgagagt ctggcgtatc gttttactgg
    25741 agatacgcca gaacagtggt tagcgagttt tcgtcagcat cgctgggatc tggaagaaga
    25801 agcggaaaac ttaattcagg aacaaagtga agatgatcaa ggctgggtct ggttaccctg
    25861 atccagatat tcgtccttcc atttcacgta attattcgcg gaatagcgta acccagcctt
    25921 ctcttcatca cttaacgggc ggatctgttt gacggggcta ccgagataca gatatccgct
    25981 ctccagccgt ttattttgtg ggaccagact acccgcacca atcatcacat catcttctac
    26041 tattgcgcca tcaagtaaaa ttgagcccat cccaaccaaa actcgattgc caatggtgca
    26101 gccgtggagc atcaccttgt gaccaacagt gacatcttcg ccaatggtta atgggttgcc
    26161 atctgggttg tacgaggatt tatgagtgac atgcaacata ctgccatcct ggatattggt
    26221 gcgtgctccg atctgtacat aatgtacatc tccacgaatc acaacgagcg gccagatccc
    26281 cacatcatca gccagacgaa cgtcaccaat cacgacactg ctatcgtcga tcattacgcg
    26341 ctgaccgatt tgtggaaaaa gatcgcggta tgggcgtaaa acatcagaca tacttacctc
    26401 agcaataaat gatttactaa tgactttggg ggcattattg gccttgtgca agtcttttag
    26461 tatgcaaaaa agcaccgttt tgtgtgcgat tgcagcaaaa agggtgaaaa aacaacaaac
    26521 agaaaaaaag atcaaaaaaa tacttgtgca aaaaattggg aaccctataa tgcgcctccg
    26581 ttgagacgac aacgtgaaac acttcacagg atggtcggaa caacgaagag
//
LOCUS       DAZGAN020000062        25162 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGAN020000062 DAZGAN020000000
VERSION     DAZGAN020000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.062"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(145..627)
                     /gene="ybiA"
                     /locus_tag="ACHKYO_RS14295"
                     /old_locus_tag="pgaptmp_002859"
     CDS             complement(145..627)
                     /gene="ybiA"
                     /locus_tag="ACHKYO_RS14295"
                     /old_locus_tag="pgaptmp_002859"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HGU4429677.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            747..2897
                     /gene="dinG"
                     /locus_tag="ACHKYO_RS14300"
                     /old_locus_tag="pgaptmp_002860"
     CDS             747..2897
                     /gene="dinG"
                     /locus_tag="ACHKYO_RS14300"
                     /old_locus_tag="pgaptmp_002860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HGU4429678.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAINDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2925..3887
                     /gene="ybiB"
                     /locus_tag="ACHKYO_RS14305"
                     /old_locus_tag="pgaptmp_002861"
     CDS             2925..3887
                     /gene="ybiB"
                     /locus_tag="ACHKYO_RS14305"
                     /old_locus_tag="pgaptmp_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HGU4429679.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4028..5113
                     /gene="hcxB"
                     /locus_tag="ACHKYO_RS14310"
                     /old_locus_tag="pgaptmp_002862"
     CDS             4028..5113
                     /gene="hcxB"
                     /locus_tag="ACHKYO_RS14310"
                     /old_locus_tag="pgaptmp_002862"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HGU4429680.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5180..5239
                     /gene="ybiE"
                     /locus_tag="ACHKYO_RS14315"
                     /old_locus_tag="pgaptmp_002863"
     CDS             5180..5239
                     /gene="ybiE"
                     /locus_tag="ACHKYO_RS14315"
                     /old_locus_tag="pgaptmp_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HGU4429681.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5342..5602)
                     /gene="ybiJ"
                     /locus_tag="ACHKYO_RS14320"
                     /old_locus_tag="pgaptmp_002864"
     CDS             complement(5342..5602)
                     /gene="ybiJ"
                     /locus_tag="ACHKYO_RS14320"
                     /old_locus_tag="pgaptmp_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HGU4429682.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(5867..6133)
                     /gene="ybiI"
                     /locus_tag="ACHKYO_RS14325"
                     /old_locus_tag="pgaptmp_002865"
     CDS             complement(5867..6133)
                     /gene="ybiI"
                     /locus_tag="ACHKYO_RS14325"
                     /old_locus_tag="pgaptmp_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HGU4429683.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNLRGSKDSQLR"
     gene            complement(6207..6884)
                     /gene="ybiX"
                     /locus_tag="ACHKYO_RS14330"
                     /old_locus_tag="pgaptmp_002866"
     CDS             complement(6207..6884)
                     /gene="ybiX"
                     /locus_tag="ACHKYO_RS14330"
                     /old_locus_tag="pgaptmp_002866"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HGU4429684.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(6926..9208)
                     /gene="fiu"
                     /locus_tag="ACHKYO_RS14335"
                     /old_locus_tag="pgaptmp_002867"
     CDS             complement(6926..9208)
                     /gene="fiu"
                     /locus_tag="ACHKYO_RS14335"
                     /old_locus_tag="pgaptmp_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HGU4429685.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9473..9733)
                     /gene="mcbA"
                     /locus_tag="ACHKYO_RS14340"
                     /old_locus_tag="pgaptmp_002868"
     CDS             complement(9473..9733)
                     /gene="mcbA"
                     /locus_tag="ACHKYO_RS14340"
                     /old_locus_tag="pgaptmp_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HGU4429686.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            10009..10935
                     /gene="rlmF"
                     /locus_tag="ACHKYO_RS14345"
                     /old_locus_tag="pgaptmp_002869"
     CDS             10009..10935
                     /gene="rlmF"
                     /locus_tag="ACHKYO_RS14345"
                     /old_locus_tag="pgaptmp_002869"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HGU4429687.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(10932..13157)
                     /gene="ybiO"
                     /locus_tag="ACHKYO_RS14350"
                     /old_locus_tag="pgaptmp_002870"
     CDS             complement(10932..13157)
                     /gene="ybiO"
                     /locus_tag="ACHKYO_RS14350"
                     /old_locus_tag="pgaptmp_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HGU4429688.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(13274..13996)
                     /gene="glnQ"
                     /locus_tag="ACHKYO_RS14355"
                     /old_locus_tag="pgaptmp_002871"
     CDS             complement(13274..13996)
                     /gene="glnQ"
                     /locus_tag="ACHKYO_RS14355"
                     /old_locus_tag="pgaptmp_002871"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HGU4429689.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(13993..14652)
                     /gene="glnP"
                     /locus_tag="ACHKYO_RS14360"
                     /old_locus_tag="pgaptmp_002872"
     CDS             complement(13993..14652)
                     /gene="glnP"
                     /locus_tag="ACHKYO_RS14360"
                     /old_locus_tag="pgaptmp_002872"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HGU4429690.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(14791..15537)
                     /gene="glnH"
                     /locus_tag="ACHKYO_RS14365"
                     /old_locus_tag="pgaptmp_002873"
     CDS             complement(14791..15537)
                     /gene="glnH"
                     /locus_tag="ACHKYO_RS14365"
                     /old_locus_tag="pgaptmp_002873"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HGU4429691.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(15941..16444)
                     /gene="dps"
                     /locus_tag="ACHKYO_RS14370"
                     /old_locus_tag="pgaptmp_002874"
                     /gene_synonym="pexB"
     CDS             complement(15941..16444)
                     /gene="dps"
                     /locus_tag="ACHKYO_RS14370"
                     /old_locus_tag="pgaptmp_002874"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HGU4429692.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(16743..17630)
                     /gene="rhtA"
                     /locus_tag="ACHKYO_RS14375"
                     /old_locus_tag="pgaptmp_002875"
     CDS             complement(16743..17630)
                     /gene="rhtA"
                     /locus_tag="ACHKYO_RS14375"
                     /old_locus_tag="pgaptmp_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HGU4429693.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKEIKIKELDIN"
     gene            17865..17930
                     /locus_tag="ACHKYO_RS14380"
                     /old_locus_tag="pgaptmp_002876"
     CDS             17865..17930
                     /locus_tag="ACHKYO_RS14380"
                     /old_locus_tag="pgaptmp_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429694.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            17983..18498
                     /gene="ompX"
                     /locus_tag="ACHKYO_RS14385"
                     /old_locus_tag="pgaptmp_002877"
     CDS             17983..18498
                     /gene="ompX"
                     /locus_tag="ACHKYO_RS14385"
                     /old_locus_tag="pgaptmp_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HGU4429695.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(18547..20130)
                     /gene="opgE"
                     /locus_tag="ACHKYO_RS14390"
                     /old_locus_tag="pgaptmp_002878"
     CDS             complement(18547..20130)
                     /gene="opgE"
                     /locus_tag="ACHKYO_RS14390"
                     /old_locus_tag="pgaptmp_002878"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGU4429696.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(20402..20530)
                     /gene="mntS"
                     /locus_tag="ACHKYO_RS14395"
                     /old_locus_tag="pgaptmp_002879"
     CDS             complement(20402..20530)
                     /gene="mntS"
                     /locus_tag="ACHKYO_RS14395"
                     /old_locus_tag="pgaptmp_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HGU4429697.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20716..21183
                     /gene="mntR"
                     /locus_tag="ACHKYO_RS14400"
                     /old_locus_tag="pgaptmp_002880"
     CDS             20716..21183
                     /gene="mntR"
                     /locus_tag="ACHKYO_RS14400"
                     /old_locus_tag="pgaptmp_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HGU4429698.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            21180..22298
                     /gene="ybiR"
                     /locus_tag="ACHKYO_RS14405"
                     /old_locus_tag="pgaptmp_002881"
     CDS             21180..22298
                     /gene="ybiR"
                     /locus_tag="ACHKYO_RS14405"
                     /old_locus_tag="pgaptmp_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HGU4429699.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(22357..23277)
                     /gene="ldtB"
                     /locus_tag="ACHKYO_RS14410"
                     /old_locus_tag="pgaptmp_002882"
     CDS             complement(22357..23277)
                     /gene="ldtB"
                     /locus_tag="ACHKYO_RS14410"
                     /old_locus_tag="pgaptmp_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4429700.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTIHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            23496..25088
                     /gene="ybiT"
                     /locus_tag="ACHKYO_RS14415"
                     /old_locus_tag="pgaptmp_002883"
     CDS             23496..25088
                     /gene="ybiT"
                     /locus_tag="ACHKYO_RS14415"
                     /old_locus_tag="pgaptmp_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HGU4429701.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLHSKGIE"
BASE COUNT         6129 a         6205 c         6696 g         6132 t
ORIGIN      
        1 tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta cgccgcatcc
       61 ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat ttatctgttt
      121 taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc gcaactccat
      181 taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc agtaagcatc
      241 gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagcg cacgcagttc
      301 tggatgctgt tcgaatttag cgcgaagtgc ttttcgcatc acctgttctt tgaccgactc
      361 ccagttttta cgcagaggct tagaacgatc gcgccccatg cgtgcggcga ccatagggga
      421 agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg cctgaaaata
      481 gtgttctgag gtgggccagg ttttcccgtc aaccttgatg ggccaggcgg caaagttaga
      541 aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt gcatgacgtg
      601 ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt attgcgagcc
      661 gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac aatattggct
      721 gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa gcgcaaattg
      781 ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt gcgccgcagc
      841 ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcga catctggcga
      901 ttgaagcccc caccggcgtt gggaaaacgc tctcttattt gattcccggc atcgccattg
      961 cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcattg caggatcaga
     1021 tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa ttcactgccg
     1081 cttttgggcg tgggcgctac gtttgtccgc gtaatctgac ggcgctcgcc agtactgaac
     1141 ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccgaac aatcaggaag
     1201 agcaaaaacg ttgtgcgaag ctgaagggcg atctcgatac ttataaatgg gatggtctgc
     1261 gcgatcatac ggatatcgct ataaatgacg atctctggcg tcgtttgagt accgacaaag
     1321 ccagctgcct caaccgtaac tgttactact atcgtgaatg cccgtttttt gttgctcgtc
     1381 gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg gcggcgatgg
     1441 aaagcgaagc ggtattgcct gacccgaaaa atttactgct ggtgctggac gaaggccatc
     1501 acctgccgga tgtggcgcgg gatgcgcttg agatgagcgc cgaaatcacc gcgccgtggt
     1561 atcggctaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag cagtttcgcc
     1621 cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt gaagagttgt
     1681 atgagcttat cgcctcgtta aacaacattc tcaatctcta tatgcctgcc gggcaggagg
     1741 cagagcaccg ttttgcgatg ggcgaactgc cagatgaagt gctggagatc tgccagcggc
     1801 tggcaaaact caccgagatg ctgcgtggcc tggcggagtt atttcttaac gatttaagtg
     1861 agaaaaccgg cagccatgac attgtacgtc tgcatcggtt gattttgcag atgaaccgcg
     1921 cgttggggat gttcgaggcg caaagcaaac tctggcggct ggcttcgctg gcgcaatctt
     1981 ccggtgcacc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag ctacacctct
     2041 ggtttcactg cgtgggaata cgtgtcagcg atcagctgga aaggctgctg tggcgcagta
     2101 ttccgcacat tattgtcacc tccgcaacct tgcgttcgct gaacagtttt tcgcgtttgc
     2161 aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg gattccccct
     2221 ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgcgttgag ccttccatcg
     2281 acaacgaaga gcagcatatt gccgaaatgg cggccttttt ccgtaagcag gtggagagca
     2341 aaaaacatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag cgctttctcg
     2401 actatgtgac ggatttacgt ctgatgttgc tggttcaggg cgatcagccg cgttaccgtt
     2461 tagttgaact gcaccgcaaa cgcgtcgcca acggtgagcg cagcgtgctg gtgggcttac
     2521 agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg catatccaca
     2581 aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa tggctgaaaa
     2641 gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt aacctgattc
     2701 agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt atctacgaca
     2761 aacgcttgct gaccaaaaat tatggcaagc gactactgga tgcattaccg gtatttccga
     2821 tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg aaatccccac
     2881 gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac tatcgcaaaa
     2941 tcattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg gatactgcgc
     3001 gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg ggcggcgtat
     3061 tgattgcgct gcgtatcaaa ggagaagggg aagcagagat gctcggcttt tacgaagcca
     3121 tgcaaaatca caccatcaag ctgacgccgc cagcaggcaa gccgatgccg attgtcatcc
     3181 ccagttacaa cggcgcgcgt aaacaggcca acctgacgcc gttgttggcg attctcctgc
     3241 acaaactcgg ttttcctgtg gtggttcacg gggttagcga agatccaacc cgcgtgctga
     3301 ctgaaaccat ttttgaattg atgggtatta cgccaacgtt gcacggcggg caggcgcagg
     3361 cgaagctcga cgaacatcaa ccggtgttta tgccagtcgg cgcgttttgc ccgccgctgg
     3421 aaaaacaact ggcgatgcgc tggcggatgg gcgtgcgtaa cagtgcacat accctggcga
     3481 aactggcgac gccatttgcc gaaggtgagg cgctgcgtct ttccagcgtt tcgcatccgg
     3541 aatacattgg acgcgtcgcg aagttcttta gcgatatcgg cgggcgggcg ctactaatgc
     3601 atggtactga aggtgaagtg tatgctaatc cgcagcgctg cccgcagatc aatctcattg
     3661 accgtgaagg gatgcgggtg ctgtatgaaa aacaggacac tgctggtagc gagttactgc
     3721 cacaagcaaa agatccggaa accacggcgc agtggattga gcgttgcctt gctggcagcg
     3781 aaccgattcc cgaatcgctg aaaatccaga tggcttgctg cctggtggct acgggtgaag
     3841 cggcaactat cagcgacggc ctggcgcgcg ttaatcaggc attttaattc ttttctcagc
     3901 cggatgagcc atgtcatccg gcttttaccc caccgcaata tgaaattcct gcatctttat
     3961 tgaccttccc acgcccggcg tgcagcataa aaatacaaca aacacataac ataaacagga
     4021 gttaaccatg gaaagtggtc atcgctttga tgctcagacg ctgcacagtt ttattcaggc
     4081 tgtatttcgt cagatgggta gcgaggaaca agaagcgaaa ttagttgccg atcatttaat
     4141 cgcggcaaac ctggcagggc atgattcaca tggtattggc atgatcccaa gctatgtacg
     4201 ctcctggagt caggggcacc tgcaaattaa ccatcatgcc aaaaccgtta aagaggcggg
     4261 ggcggcggtc acgctcgatg gcgatcgcgc atttggtcag gtcgcggcac atgaagcgat
     4321 ggcgctgggg attgagaaag cgcatcagca cggtattgcc gccgtggcgc tacataactc
     4381 gcatcatatc ggccgtatcg gttactgggc ggagcagtgt gcagcggcgg ggtttgtctc
     4441 tatccacttt gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg gtcgcgacag
     4501 ccgctttggc accaatccgt tctgtgtggt tttccctcgt aaagataatt tcccgctgtt
     4561 gcttgattac gccaccagcg ccattgcatt tggcaaaacc cgcgtcgcct ggcataaagg
     4621 cgtccccgtg ccgccaggtt gcctgattga cgttaacggc gtgccgacga ccaatccggc
     4681 ggtaatgcag gagtcgccgt tgggttcgct gttgaccttt gccgaacata aaggctacgc
     4741 ccttgcagcg atgtgtgaaa ttcttggcgg ggcgctttcc ggcggtaaaa cgacgcatca
     4801 ggaaacgtta caaaccagtc ccgatgccat tcttaactgc atgaccacta tcatcatcaa
     4861 cccggaactg ttcggcgcgc cggattgtaa cgcgcagacc gaagcctttg ccgagtgggt
     4921 gaaagcctcg ccgcatgatg atgataagcc gattttgcta ccgggcgagt gggaagtgaa
     4981 cacgcgtcgc gaacggcaga agcaggggat tccactggat gcgggaagct ggcaggccat
     5041 ttgtgatgca gcgcggcaga ttggtatgcc ggaagagacg ttgcaggctt tctgtcagca
     5101 gttagccagc taaaaaaaag cccgtccagt ggcggacggg caaacaaggg taacatagga
     5161 tcaatgaggg ttagagcata tgcgtctgtc ggcaaacaga cagggaaata cttgtgctgg
     5221 acgtagcgta aacgcctgat ccgtccaacg gttcggattt tgtaggcctg ataagacgcg
     5281 gtagcgtcgc atcaggcatc tgttgccgga tgcggcgtaa acgccttatc cttcctacga
     5341 attacttata aattaccgca gtaccgctta atttattgtt gttggtggcg gaagtgatgc
     5401 tgtatccact ggcaccggct gccgcggctt tctcagccag tttcgcttcc agggcatcga
     5461 gggtggatgc gccatcggca gaaaccacgc cgattttatt catgttctgt gcctgggatg
     5521 ccgttaccgg ttccgcggca aacacgccaa atgacagagt tgaaagagcc atagcagcaa
     5581 caacagtatt gatagttttc ataattaatc tctcgcaggt gatttttgta taagtgacgt
     5641 tgtttcgtcg atgtgatgag tatcacgttt ttttacgaga gataaaatcg aagagaattg
     5701 accgccttgt tcaaataaat tgattgatat ctaacatact gataaaccat ttatttgttg
     5761 tacttatgga cttgttttat gcagcgtggc agtcacgaca gcgttaaagg cacggaatgc
     5821 cacgcaaaat acaaattata ctcgctatac atctcgccag ttgtagttaa cgtaactggc
     5881 tgtctttcga acctctgaga ttatatcctg tatacgctgg tttttgtaaa tctttctcct
     5941 gctgacaatg aatgcataag cgcacgccag gaatggcttc ccgacgggct tgcgggatgg
     6001 gggcaccgca ctcttcacat tcatccaggc tttcgccacg cggaatttca ccccgagcgc
     6061 gggcaatcgc atcttcaatt gtactgttga tctgttcgtt gacggcgtca tcgttagccc
     6121 aaccggatgc catatcgtcc tccccatatc aatgcttgta cagttaagtg tagctaatcc
     6181 agggacgaac ttgggcagtt caagcatcag atctccgacc attcccgcag cagattatga
     6241 taaagattaa gcagcgacag gatctcttca ttttcaccat agcggctttt cagcgactga
     6301 atattgttgt ccagttcaaa cagcatggcg cgctttttat catcacggat catcgactgg
     6361 atccacataa atgatgccac tcgtacgccg cgggttacgg gtgtcacgca atgcaggctg
     6421 ctggagggat acaacacgag atcgcctgcc gggagtttta cccgatgttg tccgaaggtg
     6481 tcattaacga ccagttcgcc gccgtcgtag ctttgtggat cgcttaaaaa cagcgtggca
     6541 gaaaggtcag tacgcatcca gccgttttgt ggatggctgc gtactgcgcc atccacatgg
     6601 aaaccatagg tttcattgtt ctgatagcga ttaaacagcg gcgtggaaag ggtacgcggc
     6661 aaggccgcgg caaagaataa agcatgttgg ttaaccgcgt tcagcacctc attttgcagg
     6721 gcggcgtata acgtgctgcg ggtgtcgacc tgttgattgt tcttaacttg cgcaccttgt
     6781 gcgccggtgg tgacgcgtcc atccacccat tcggcttgtt ccagttgttc gcgaaaccgg
     6841 gcgacgtcct gtggcgataa cacgccggga atgtggtaca tcatacaagt tctccaaaaa
     6901 gtggggcctg cgccccacat ctgaatcaga aatgcatatt ggctgtgagc aagaaggttc
     6961 ttggctcgcc cgggtgataa cggtagccgc tcttgttgat tgaggcgacg taatcggtat
     7021 caaacaggtt gtaaacgttt agctggaagt cgagattgcg attaacccga taccccagtt
     7081 tggcatcggc gacccagtaa ccttcggtaa acgctggcgt tcccaccgcg ccgtctgaac
     7141 ctttatgcat actgccgata tagcgtgcgc ccgcgccaac agagatatca tctgttgcct
     7201 gatactggct ccataaggtg aaggcgtgct ccggggtata cggcagcgat gaggaaccat
     7261 cctgggcaac atctttgccg tttttgatgg ttgctttttg ctgggtatag ccgccaatca
     7321 cctgccacgc gggcgtgata ttcccggcca cggatatctc atagccttcg acacgtttct
     7381 taccgtattg cgagtaagtt ccgtcatcat tttgctcaac ttcattttcg atatcagtac
     7441 ggaatagcgc ggcggtgagc aacaggcgtt tatccagaac ctgccatttg gtgccaatct
     7501 cgctggtgtt ggctttttgc ggtttaaaat cggtgcggtt ggcactgtta ccgctgccag
     7561 actgcgcaag ggcgaagttg ttgccgcccg gaggctgctg ggaaacggca tagttaatat
     7621 agacattacc gttttccgtc aggtgataca gcgccccggc tttccagttc accagattgc
     7681 ccgacttggc ggtgtcgacg gtggtgaccg gagaaccttt tgccacacca gctgggcagg
     7741 tgatggcacc gcgtccgctg ccgccgcagg cggtggcact gtcatattca gtatgataat
     7801 tatccagacg gatcccgccg ttcagctcaa aatcacgggt gatttgcagc gtatcgaagg
     7861 cgtaaattgc gaaggtatcc gtctgaccat tggcgtttgc gccgttgcgc gtcaggccgc
     7921 cgggatgaat gctgctgtca ggatgataaa tatttaccgc gggtaacgtc accggattaa
     7981 cgccgtagtt agtctgcgtt tcacgggtaa attccacgcc ggtactgaca tcatgaccga
     8041 tagaaccggt atagaacgtc gaggtcaggt tggtctggtt ggtgagaatt ttattactca
     8101 catctttggt attcgccgtg cgtgaccagg tccagctatt cacatcgctg gtgggctgag
     8161 taatattcga cgccccgccc ataatcgccg tcatcaggta atcctgcttt acgcgcgacc
     8221 aacgggtagt attgcgaatg gtggtgttat cgttgatgtc gtgctcaaaa cgcatggtgg
     8281 cggtgtcggt ggtcgaatcg tcgtaatcgg aatccgtgcc gtaaaagtta tgagtatcaa
     8341 cttttccgga atgattcaga gccgctgttc ccgcagatgg ggcagaataa cccggcaaac
     8401 cgatggtcgg aatgccgccg tctggcgtgt tgtgctgggt gacatgcaga taattaagat
     8461 acaaacgatt cgctgtacca aggccaaaag cgatagaagg ggcgacgccg taacgctcat
     8521 ttttgacttt gtcgcgtccg gcatcgtgtg ttttttcgcc catcacattc aggcgcaccg
     8581 cagtggtatc accaatgacc tgattgacgt ccagcgtgcc gcggcggaac caggcgctgc
     8641 caatactggc ggaggcgtca atgccggaat cattgcgcgg ctgcttgctg atcatattga
     8701 tcgagcccgt cggcgcgctg cgcccgtagt cggtgccgga aggcccttta atcacttcga
     8761 cctgctcagt attgaaggtg tcgcgcgaga cgctgccgat atcgcgaatg ccatcaatat
     8821 aaatactgtt agaggtatca gcaccgcgca tataaatggc gtcgccagtg gtggagttac
     8881 cgttctcacc cgcaaaaaac gcacccacgc cggggacgtt tttgagcgcg tcggtaaggt
     8941 tggttgcgcc ctgatcttta atcacttgtt cagaaatcac cgtcatcgtg cgggtagtat
     9001 ccgctaccgg acgcgagaat ttcggatcgg cagattgttg tggcgcataa agcgaaggcg
     9061 ttgatgcttc gacaaccagc gtgtcatccg cgttagtttg cccttcggcg gcgagtgcct
     9121 gagccacagg cgtgatgcca atacaaagac cggcaaagaa cgtgagcgaa tgaaattgtc
     9181 tggcagggaa attgcgattg ttttccattt tgcaggtgac ttttttttat atgttgaaat
     9241 tttcactgcc acgagaaagc ctctctgcgg atgcgatgcc actgttaatc tcgtgatagt
     9301 gttcgtgcaa gtgataataa ttttgataag cattatcact tggcgcgatt ttctatcacc
     9361 cattgcgcga aaaagaaata catttattta cattgcgttc atatttgatg ttttttgatt
     9421 acaaaacagg cgaaaactgc atccagaggc gaatgcagtt acgttggtgg ggttatttgt
     9481 agatggttgc agtaccgaac atctggtcat ttccgcctgc ggaattaatg acataacctt
     9541 tcgcgccttg ctcgcgcgct ttttctgcca gtttatcctc cagatcgctt aggtttgatg
     9601 cgccagttgc cgaaacggta ccggcgggcc gtagctgacc gctgtccagg ttactcatcg
     9661 gttgcgcggc ataagccgtg agagagattc cgctcaggac ggtggcaatc agtagtgtga
     9721 ggcacttttt cataatgaca tcctcataaa tacaacgggt agtgcctgat aagtgtaggt
     9781 cgcctgggcg atgaaaatga cgcaaaaaat tgacagagat gtgcttattt tttgaacgat
     9841 atatttttac aaaataagac aaatcaatga catgcataat gctgattttt atgatgaatt
     9901 gccataaacc atagccatgt taaggttttg gggaatgata aacgcagcca gatatttacc
     9961 ctgtcgcgtt atgcgggtat cttacgccgc tgcttaaagg agaatgctat gtccgcccag
    10021 aaaccggggt tgcatccgcg caaccgtcat cacagccgct acgatctcgc cacgctttgt
    10081 caggtcaatc ctgaactcag gcaattcctc acgcttacac ccgcagggga gcaaagcgta
    10141 gactttgcca atccgctggc ggtgaaggcg ctcaataagg cattgctggc ccatttttac
    10201 gccgtagcga actgggatat cccggacggt tttctctgcc cgccagtacc gggccgggcg
    10261 gattatattc atcaccttgc cgatttactg gcagaagcaa gcggaacaat tccagctaat
    10321 gccagcattc tggatatcgg cgttggtgcg aactgtattt atccgctgat tggcgtacat
    10381 gaatatggct ggcgttttac cggtagtgaa accagcagtc aggcgttaag tagtgcgcag
    10441 gcgatcatca gtgctaatcc ggggcttaac cgtgccattc gtctgcgtcg gcaaaaagag
    10501 agtggggcga tttttaacgg catcatccat aaaaacgagc aatacgacgc gaccttgtgt
    10561 aacccgccat tccacgattc cgccgctgcg gcacgggcag gtagtgagcg taaacgccgt
    10621 aacttggggc tgaacaaaga cgatgcactg aactttggtg gtcagcaaca ggagttgtgg
    10681 tgtgaaggtg gtgaagtcgc ctttatcaaa aagatgattg aagagagcaa aggcttcgcg
    10741 aagcaggtga tgtggtttac ctcgctggtt tctcgtggtg aaaacttacc gccgttgtat
    10801 cgtgctctga cggacgtggg cgcggtgaag gtggttaaaa aagagatggc ccaggggcaa
    10861 aagcagagtc gctttattgc ctggaccttt atgaacgacg agcagcgccg ccgttttgtt
    10921 aatcgccaac gttaaagcgt tggctccccc ggcggcagcg gttcagccgg ggccgcgccc
    10981 ggagcaggca gcacctgata agtctgcact ggcgcgcgaa cacctgccag gtcgaaatgc
    11041 tttttcacct ggctgtcgag ggcaaagcgg accgtccact gtttgagtgg cagcgtggtg
    11101 aacgaaacac gcagtgtaaa cgcggtattg cttaagccga caatcccggc aaagtttggt
    11161 tcaccaataa ccagcccgcg aatttcttcg ttttccatca attccgctac cgcatctttc
    11221 agtgcctgat tggctttatc agcatcttca tggcgatcaa catcgtagtt tgccaccacc
    11281 gaaccaatgc cgcggacgaa gttagcaaag gtggttatcg aagaccaagg aatgatgtga
    11341 tacgccccgg tatcctgacg cacgcccacg gagcgaatcg acatccgttc cacagtgccg
    11401 gtcaacggcc cgatagtaac caaatctcca gtgttcatgc cgttttcaaa ctgaataaat
    11461 accccggtga taatatcttt caccagcgtt tgcgaaccaa acgagatagc cagacctaat
    11521 gccccagcac ctgccagcaa tggcgcgata ttgacgccga tttccgacaa cacaatcatg
    11581 atggtgatgg tactgataat caccgccagc gcgttacgga acagcgtcag cagggtacgc
    11641 gtacgggcgc tgggtagcgg gcggccatga atatccgaag ccagccggtt ttcgatcaaa
    11701 ctggcgagca ctgtccagcc aaccgccgag aagaaaagaa tgagtgcgat gcggatcagg
    11761 atatctacgg ttttctgccc cgcgccgttt tgcagccagt tccagaaatc gaacaatccc
    11821 catgcgctca acagcaacat taccgccacg cagacggtca gaatacgcgc cgttttcagc
    11881 gccgccgaaa gccagccatt cagccgtttt tgtagctccg gatagttacg ctgagtatgt
    11941 ggcgagaggg tgatggtttt cgccagccag cgggaaaaca taccggaaac aaacgctgcg
    12001 ataccaataa tcgccaggct gcgcaccgtt gcacccatca taaatttcag gctgttgccc
    12061 ggatcgaaca acgaaaagaa aaacagcacg ataaaatagg cgcttgccag ccagtgccac
    12121 accagcgcaa aggcgcggat aaacaggctg aaaaaggcca gcgaatgctc cgcaaagttg
    12181 agcaaatgtt gggtaatctc ttttttattg cgaaagatca ggtacaacgc ccagacggtc
    12241 atgcacagca taatgatgac gttcgccagc gcacctatct gtacattcac ctgattagag
    12301 ataatcggca cggccacaat caggccataa cctatcaaac tgcttaacca gctcaggcga
    12361 cgactccagt aacgggcgct ctcgtcctga atcgtgaacg ggcgcagttc agccacgttt
    12421 gggcaaaaaa tcaggcgtag tacggctttg aaaaattcaa tcagggcaaa ggcattgaga
    12481 aacaaacttt gttggaaagc gatggtgcga ctgcctgcat tcaggttgtc gcttaatacc
    12541 tggccgacaa ataatgtcag ggccagtaac agcaggtcga taataaacgc cccgataatc
    12601 atcgccggaa gctgcaacca gttgctgcgc tcacgatttt tttgcctcgc ccactgaccc
    12661 attttgcgat acagcggcag tgcgcacagg cgaatcagcc agtaaaaacc aaacactaat
    12721 accgctaaca ttgaaaaatg ggtcagcgca ttgctgaagg tttgtggatt aaatggctta
    12781 tgcggggagc cggtgatatt gcgataaagt tgcccgaagc gggcggaaag ggcttcacca
    12841 taatggcggc tgacttcggt gaccttttgc agcacggttt gctcttcaac cagcgtcggc
    12901 ggcacgatct tcgggaccgg ttccgcaggg ggcgtcgcgg cgacagtacg caactggtcg
    12961 atcaattctt tacgcgaggt gtcgttatcc agcacatccg ccagtgcgcc ataggccgct
    13021 tttttttgtt cgatatccgg ttccggggcc ggttcagtcg ttgagtccgt tgtcgttgtg
    13081 gttgtaacgc cgggtatgga taccgcgtgg gcaggtgcgc ccagcaggca gaagaggatg
    13141 aacaggatcc accgcatgac tcctccagtg agaaaacaga gcaaaaagat aagtatagat
    13201 gctgggggag gggcgatttt aaatgagagg aatctggtgt gcctcccttt cgggtgaaag
    13261 ggaggcagag aagttaagag acgtgctgca aaaattcctg caagcgctgg ctcggcgggt
    13321 tcttgatcaa tacctgcgga tcgccatctt ccgcaatccg gcctttgtcg ataaagatca
    13381 gccgcgaagc tactttctcg gcaaaaccga tttcgtgggt cacgatcacc atcgtcatcc
    13441 cttcttcagc cagatcctgc ataaccttca gcacttcatg gcgcagttcc gggtcaagag
    13501 cggaagtcgg ttcatcaaac agcatcattt tcggcttcac cgccagcgcg cgggcaatcg
    13561 ccacacgctg ctgttgacca ccagaaagtt cggaagggta gtgatgtgca cgttctgcca
    13621 gaccgacttt cgccagcagc tcacgcgcca gtttttccgc ctcttctttg ttcgcgccac
    13681 gcacgcgtag cggaccaaac atgacgtttt ccagcgccgt cagatgcggg aagaggtaaa
    13741 actgctggaa caccatacct gcttcctggc gaatcaggcg ctcgtcaact ttcggatcgt
    13801 taaccttcag gccatcgaca atcagatcgc cggaggtgat ttcttccagt ttgttgatgc
    13861 agcgcagcag ggtcgattta ccggaaccgg acggcccgat aatcaccacg acttcgccct
    13921 gggcaatgtt caaatcgata ttgtgcagca cctgggttgg gccaaagtgc ttggagacgt
    13981 ttttaaattc aatcacagga ttttcatcct tctttccaga cgacgcagaa taaagctcag
    14041 caccagggta ataatcagat agaacaccgc cacggcgctc cagatctcaa gggcgcggaa
    14101 gttaccggca ataatttctt gcccctgacg ggtcagttcc gccacgccga tcacaataaa
    14161 cagcgaggtg tctttaatgc tgatgatcca ctggttaccc agcggcggca gcatacgacg
    14221 cagtgccagc ggtaaaatga cgtagcgaat ggtttcccaa cgtgaaagac cgagcgccag
    14281 tcctgcttca cgaaaacctt tgtggataga cagcaccgca ccacgcgtga tttccgcaat
    14341 gtacgcgccg gagttgatca tgatggtgac caccgccgca gtaaatgggt cgatgcgtaa
    14401 gtcgttaaac gccatcggca gggcgaaata gataaacatc acctggacga cgataggtgt
    14461 gccgcggatc acttcaataa agaccagcgc gacgtggttg gctatccaac caccgaaggt
    14521 gcgtgcaaaa cctgccagca atccgattac cagaccgcct gccagaccga ggaccgaaat
    14581 ccacagggtc attttggcac cttcaatcag aagcggaatg gcaggccaga tggcactcca
    14641 gtcaaactgc atatgttgtt cctgttaccg tggtgtaaat aatcaaattc agggtaatta
    14701 atggccccgg agcgggtttg cgcccctcac cctaatcctc tccccagagg ggagagggaa
    14761 ctgccagtgc gttttacagg tgtagcgtta ttatttcggt tcagtaccga accatttttt
    14821 gtagatttcg ttgtaagttc cgttctcgcg cagggttttc aacgcgccgt tgactttgtc
    14881 acgcagctcg tcgctacctt tcgggaacgc aataccgtat tgctgcgctt ccagagagtc
    14941 acctaccgct ttgaactgac cgttaccggc ggttttgatg aagtacagaa tgtttggcgt
    15001 atcgtgcaga acggcgtctg cgcggttggt gcccagttcc atataggcgt tatcgatgtt
    15061 cgggaactga cgcagatctt tagttttgat gtttgctttc gcgtaatcaa cggagccagt
    15121 accgctcttc acagcaacca ctttcccgtc gagatctttc acgcttttca catcgttatt
    15181 gttagctttc accatcacta acaggccgct tttgtagtag ccgtcagaga aatcgatcgc
    15241 ttttttacgc tcgtcggtga tggtaatgcc cgccagcgcc agatcgacgt ttttggtttg
    15301 cagtgccgga atgatcccac tgaaatccat cggcttcagt tcgtaatcca gcttcagctc
    15361 tttagcgatg gccgcccaca gatcaacgtc aaagcccaca tatttatcgc cctgtttaaa
    15421 ttcaaacgga acgaaggcgg tatccgtcgc gacaactaat tttttatccg cggcatgaga
    15481 agaaaccgca aaagccaggg tcagtgcagc cagtgaaact tttaatacag acttcatagc
    15541 atttcctttt ttctatccac gggacgatcc cctgcgtgac attcacatat atgaaaaaat
    15601 cgtgccagtt ttgcaactcc ttgatttttc gagatgcggc aaaaacgtga tgcacgattt
    15661 atagggcaat accctgaaga tgcaccattc tggggcacca atctggtgcg ctaaaattgt
    15721 gcactcaaca cagtgattat ttagcgtaaa acctgatgaa aaaacaatct ttatgtaacg
    15781 attgtgtgat gatgtggata caaaaaattt acttaatcag ctggagatag cagatggatg
    15841 cactaaataa gtgcgttgag gtgggctgca aaacaaaacg gcctcctgtc aggaagccgc
    15901 ttttatcggg tactaaagtt ctgcaccatc agcgatggat ttattcgatg ttagactcga
    15961 taaaccacag gaatttatcc aggtcgcgag acgcggcggt caggatatct gcggtgtcgt
    16021 catctttcgc ttcgccaatc gctttgcgta cgtcattagc gacgattgcg taacggtcag
    16081 ccagttcttt caggtgatcc tgaacgttgt ggatgtccag cgggtaactt ttcagcgggg
    16141 ttttgctgtt gataacttga gtggtcccca gagctacacc gcccagctgc actgcacgtt
    16201 ctgccatggt atccagatga tcgatcagtg cggtgcggaa gccatccagc atttcatgta
    16261 cggcaatgaa gttagcgccg cgcatgttcc agtgcgcttg tttggtaatc aaagaaagat
    16321 caataaactg gataacctgg cgattcagca actctactgt tgcttttttc tcgctgtcgg
    16381 agacatcgtt gcgggtataa agcagattgg tcgcttttga tttaactaat ttagcggtac
    16441 tcataatttc atatcctctt gatgttatgt cccagtaatt aacgagacta agtatagcac
    16501 cggctatgtg ttccgctatt ctggctgttc ctatcacact aatagtggta acaagcgtga
    16561 aaaacaaaac taataaagat tcaatgagtt agatatattg ataagaacaa ttcttattta
    16621 tctgctcgcc aggaattttc ggaataaata tgcttttgca taattcgata tataaagacg
    16681 gtgtagagga aaagtagcga gaaattctgc atggttatgc ataaccatgc agaaatgtaa
    16741 atttaattaa tgtctaattc ttttattttg atctctttgc gtactgtcag cgtagacccc
    16801 attgaagcgg cgatgatagc gccgagcgcc agcagctgta tgggtgtcag tgtttctccg
    16861 aggaaaatca tcccggaaac ggcagccagc gccggttcca tgctcatcag cgtaccaaat
    16921 gtccgtgttg gcaaacgggt gagggcaatc atttccagcg aataaggcag agcggtcgag
    16981 agaatagcga cagccagacc caatggaata accgaccagt gccagagtgc ttcaccagcc
    17041 tgaagcgctc caattggcac gaaaattaac gctgcaatca acgaaccaat tgccaccgtc
    17101 gcagggccat gttccgctcc tgcgcgttgc ccacttaaaa tgtaaatagc ccaacaagcc
    17161 ccggccccca gtgccagcgc acagccggtt aaatcgacat gggaaacgtc ttgccccagc
    17221 ggtagcagga accacagacc aagaaccgcc agcacaaccc agacgaaatc taccgggcga
    17281 cgagaagaga acagcgccac cgccagtggt ccggtgaact ccagcgccac cgcaataccc
    17341 agcggtactg tctgaataga aagataaaaa agataattca tcccacccag cgaaacgccg
    17401 taaaacaaca gcggtaaccg ttgctctttg gcaaagcgca gtcgccatgg cttaaagaac
    17461 gcgatgagga tcagcgttcc taatgccaga cgcagcgcag tgacacccgg tgcgcccacc
    17521 agaggaaaaa gtgacttagc taacgaggct ccaccctgaa tagacgccat ggcaacgagc
    17581 aatatgatta ttggtaacca gaccggcatt ttacgtaatg aaccaggcat cctttctccc
    17641 acaaatatct agacttaagt aaagcgtgga gtgtactgga tatacccaat gctggttgag
    17701 catttgttga aaaaattttc ccccgttttg actaaaatgc gccaggattg atggaatcat
    17761 tagtctggtg attaggaata atctggatga atgacaggga aaacatgcgt aatacttacg
    17821 cagttctctg aaaaagtgat ttaaatttag atggatagcg gtgtatggaa acgttctgtt
    17881 acatgaaatg gcccgttaga catcacaaat cgcgaagagt ttcccattaa tttttgatat
    17941 atttaaaact taggacttat ttgaatcaca tttgaggtgg ttatgaaaaa aattgcatgt
    18001 ctttcagcac tggccgcagt tctggctttc accgcaggta cttccgtagc tgcgacttct
    18061 accgtaactg gcggttacgc acagagcgac gctcagggcc aaatgaacaa aatgggcggt
    18121 ttcaacctga aataccgcta tgaagaagac aacagcccgc tgggtgtgat cggttctttc
    18181 acttacaccg agaaaagccg tactgcaagc tctggtgact acaacaaaaa ccagtactac
    18241 ggcatcactg ctggtccggc ttaccgcatt aacgactggg caagcatcta cggtgtagtg
    18301 ggtgtgggtt atggtaaatt ccagaccact gaatacccga cctacaaaca cgacaccagc
    18361 gactacggtt tctcctacgg tgctggtctg cagttcaacc cgatggaaaa cgttgctctg
    18421 gacttctctt acgagcagag ccgtattcgt agcgttgacg taggcacctg gattgccggt
    18481 gttggttacc gcttctaatc actttggtga tataaaaaat ccgcctctcg gggcggattt
    18541 ttgtttttaa ggcttcgggt cgaaaatatc ggttccgaga tggttgtaat ccaccttctg
    18601 taactggaag ttggtgatgt atatcgaccc ggctttcttc tcagatataa acgggtattt
    18661 gatgttaatt tccttcgctt taattcccgt ccactgggag aaaaaaccta agaagtcatt
    18721 ggctgagcgg cgggctttaa tcacccgatg cgctttatcg tcgctggaaa tgaccataaa
    18781 aggcacctgg aaattttgct gatacttatc atcatgggca aggtattgca cgtctttgcc
    18841 gcgctcttta aaggccagac cgtggtcaga aaagtaaacc agcgagaagc tgctgccgct
    18901 gttgcgtaac tgatcgtaca gcttgcgcag taaatcgtcc gtttgcgtca tggtatagag
    18961 atagcacgac gtttctttcg actgcacaaa ggtttcgtat ttgccttgtg tcctgtcgca
    19021 ggcctgcgga tgtgagccca tcagatgtag aacaatcagc tgcggttgcg agtgctcttg
    19081 cgccagcact tgagcggtca tatccagtaa cgcttcgtct ttcgtgtttt tatctgcttc
    19141 aaaattacct tctttcagga agtacacttc atctgcacgt ttggcgatgc tggcgatagc
    19201 agtatcgtat tcgccgattt ggccctggtt ggaaaaccac caggtctgga agcccgcgcg
    19261 atttgccagg gtgacaaagt tatcctgaaa ctgtggtttg ccatcgacta cgcgattgag
    19321 cgttaagcca agcgatttct gcgtggagcc actggcggca atgtagtcag caaatatcaa
    19381 accgttaacg ctgctggcaa acggggtatt gtcccagtga ccgccaaagg caccgagggc
    19441 atcgcgacgc gcgctttcac cgatcaccac cacataggtc tgatacttcg gcttaacggc
    19501 agtgaccgtc caggtatctt tcattccgga aagttttgcc atccgttctt gctcttcaat
    19561 cacctcgtta ttattgacga tgacatcctt agcaaaacga aataccggat agccagtatc
    19621 tttcagttta aatacgccac cccaggccag gttttgcacg ggagcaacaa aaaatgtcgc
    19681 cacactgaat accagacaca ggctgtcgaa ggtattccag cgcgcttttt cactctcttt
    19741 ttttcgcctg attgctatta cgccgagcac aaaaataaat atgccgacca tgtagctgta
    19801 ccacggaaaa atcgtcagga tttcggtcga ctcttccata ttggtggagt gcaatgccag
    19861 caatgtattg aagttaggcg cgccgtaggc ctgagcaaaa gggaaataac aagccgccac
    19921 cagcgaactg ataccgacca gaactttttg tacgcgaggc aatgttcgcc atagcaaaag
    19981 caaaatagcc gtaaacgcag cggtgtagag caaactgaag gggtaaccta agccgaggtt
    20041 aattaatagc gactgtaaaa agtagaacgc agtccacggg ctaaataccc ggctacgggt
    20101 aacaagcgat tctttgaggg ttaaattcat atgccactat cgtaacaaac gccatgtgct
    20161 taccctggcg tcaagggtca atacctgcaa gagtgcgtga agaggggaaa ggggtccgca
    20221 tccgcgagcc gcaatatacg cagggctgca agaagataga gcgagccgcc gttcaggtca
    20281 actactgacg aacaaatctt ttgcgaaggg gattgcaaat ccgacaaaaa gtggggtttt
    20341 tcagaccaaa acgcgttatg cctgcatgaa agcaggagtg aagcggcgta ccgcgacgcc
    20401 gctatttatc ggaaggttta tcttgctgcg gtttgttgtt gaccatatcg cacaacatag
    20461 agagcagcat taaccgtact ttaaagggag aatgactaaa cacgcgcata cacctcttga
    20521 actcattcat aagacctcct gacttgctaa tcccgtcgat ccttgaggga tgattgcatt
    20581 acatacagat atagcacagg ctatattata tagctattgc taaaacgtta attttttgta
    20641 ccttcgcaac tctggtttac aatgtgcgca cgaaatgaga acgctatgta tgcgtcacca
    20701 taatgaggaa gcacaatgag tcgtcgcgca ggtacgccaa cagcaaaaaa agtgacgcag
    20761 ttagtgaacg tggaagagca cgttgaaggg ttccgccagg tcagagaggc gcatcggcgc
    20821 gagcttattg atgattacgt tgagctgatt tctgacttga tcagggaagt gggggaagct
    20881 cgtcaggtgg acatggctgc tcgtctggga gtttcgcaac cgacggtggc taaaatgctt
    20941 aagcggctgg caaccatggg gctgattgaa atgatcccct ggcgtggcgt gtttttaacg
    21001 gcagaaggag agaagctggc gcaggaaagc cgcgagcgac atcagatagt cgaaaatttc
    21061 ttgttggtgt tgggcgtcag tccggaaatc gcccgtcgcg acgcggaagg catggagcac
    21121 catgttagtg aagagacgct cgacgctttt cgtttgttta cccagaaaca cggtgccaaa
    21181 tgagcctgcc ttttttacgc acgctgcaag gcgatcgttt ttttcagtta ttaattcttg
    21241 ttggtatcgg attaagcttt ttcgtgccct ttgcaccgaa atcctggcct gctgctatcg
    21301 actggcacac catcatcacc ttaagcggcc tgatgctgct gaccaaaggt gtggagttaa
    21361 gcggttattt tgatgtgctg gggcgcaaaa tggtgcgccg ctttgctacg gagcgtcggc
    21421 tggcgatgtt tatggtgctg gcggcggcgc tgctttctac ctttctgacc aacgatgtcg
    21481 cgctgtttat tgttgttccg ctgactatca cgctaaaaag actgtgtgag atcccggtta
    21541 atcggctgat tatttttgag gcgctggcag tcaacgctgg ttcgctactg acgccaattg
    21601 gcaacccgca aaatattctt atctggggac gttctggtct ttcgtttgcc ggatttattg
    21661 cccaaatggc accgctggct ggcgcaatga tgctgacgct cctgctcctg tgctggtgtt
    21721 gtttccctgg aaaggcgctg caataccata cgggggtgca aacaccggag tggaaaccgc
    21781 ggctggtgtg gagttgtctg gggctgtata tcgtctttct gacggcgctg gagttcaaac
    21841 aagagctgtg gggactggtg attgtggcgg caggctttgc gctgctggca cgtcgcgtgg
    21901 tgctcagtgt ggactggacg ctgctgctgg tgtttatggc gatgtttatc gacgtccatt
    21961 tactgaccca gcttccagcg ttgcaaggcg tgttgggtaa cgtgagtcat ctatctgaac
    22021 ccgggttatg gttaacggca atcggtttat cgcaggtgat cagtaacgtg ccgagtacca
    22081 tattgttgct gaactatgtg ccgccgtctt tattactggt atgggcggta aacgtaggtg
    22141 gctttgggtt attacccgga tcgctggcaa atttgattgc gctacgtatg gcgaacgatc
    22201 gccgcatctg gtggcgtttc catctctatt caataccgat gctgttgtgg gcggcgttgg
    22261 tgggatatgt tttgttagtt atactcccgg ccaactaggg tctggcaaat aaaaaaggcg
    22321 gattatgagt ccgccttttg ctttaagttt tgaagattaa ttcagacgaa ccggcatccc
    22381 ggagcggttt ttaatcgctt catcaagaac aacctggtca acatctggct gaccggtcac
    22441 tgtttgcacg ctcttcgtca gggtaattgg cacaatttcc tgaccttcaa actgggcttc
    22501 ggtggtagac agcgggttat ggacttcaat ataacggctg ccgtctggct cggtggtcgc
    22561 ttttaccggc tcatcaataa actgtacgcg ggtaccgacc ggtactttct cgaacaggaa
    22621 tttgatgtct tcgttacgca gacgcacaca accatgactt acacgcaggc cgataccgaa
    22681 gttggcgttg gtgccatgga tagcatacag gcgaccgatg tagagtgcat acagacccat
    22741 cgggttatcc ggacctgccg gaacgacagc cggaagcggt tcgcccgcag cgcggtactc
    22801 tgcgtgcatt ttggcggtcg gcgtccaggt cgggcctgct ttcttacgct caactttggt
    22861 ggtccagttg ataggcgtat ctttgcctaa ctgaccaatg ccgatcggca gcacgataac
    22921 ggtgttggtc cctttcggat agtaatacag acgcatctct gcactgttaa tgacgatgcc
    22981 ttcatgaatg gtatccggca ggatcagctg ctgcggaatg ttcagtacag taccgccttt
    23041 cggcaggaag gtatccacac ccgggttcgc ttccatcata ttggaaagcc ccatctggta
    23101 ctccgcggca aaatactcca gcggctgagt gttaccttca ggaatggtga tcacctgatt
    23161 ctgaccaacc aggcgactcc cgtcggttgg cagaggataa gttaccgcag aggcggtact
    23221 gcaaaagccg acaacagcga aggccgctgc gaataatgtt ttcaatttca tattcatgtt
    23281 aagcgagatt ttgtgcctgg caggccattg ggttgagaat attagagtat tggaagcgca
    23341 ttataagttc attccagctc acagtgaaat cagatgtgta cgaaatcaca ttttttgcct
    23401 ttggcttgag tgtagacctt aagcgaggag caggatcttc tttcagactt atggcataat
    23461 gcgcggtttg tcatatctct tttcaggata cgcctgtgtt agtttccagt aacgtcacca
    23521 tgcagttcgg cagtaagccg ttgtttgaaa acatttccgt caaatttggc ggcggcaacc
    23581 gttacggcct gattggcgcg aacggtagtg gtaaatccac ctttatgaag atcctcggcg
    23641 gcgacttaga gccgactttg ggtaacgttt ccctcgatcc caacgagcgc attggtaagc
    23701 tgcgtcagga tcagtttgcc tttgaagagt tcactgtgct ggatacggtg atcatggggc
    23761 ataaagagtt gtgggaagtg aagcaggagc gtgaccgcat ctatgctttg ccggaaatga
    23821 gtgaggaaga cggctataaa gtggccgatc tggaagttaa atacggcgaa atggacggtt
    23881 actctgcgga agctcgcgcc ggtgaattgt tgcttggcgt gggaattcca gtggaacagc
    23941 actacggccc gatgagtgaa gttgctcctg gctggaagct gcgtgtgctt ctggcgcagg
    24001 cgctgtttgc tgatccggat attctcctgc tcgacgaacc gaccaacaac ctcgacatcg
    24061 acaccattcg ctggctggaa caggtgctga acgagcgtga cagcaccatg atcatcatct
    24121 cgcacgaccg tcacttcctt aacatggtct gtacccacat ggcggatctg gattacggcg
    24181 agctgcgcgt ttatccgggt aactacgatg agtacatgac ggcggcgacc caggcgcgtg
    24241 aacgtctgct ggccgataac gccaagaaga aagcgcagat tgctgagttg caatctttcg
    24301 ttagccgctt tagcgccaac gcctcgaaat ctcgccaggc aacttcgcgc gcgcgccaga
    24361 ttgataaaat caaactggaa gaggtgaaag cctccagccg tcagaacccg ttcatccgtt
    24421 ttgaacagga taagaaactg ttccgtaacg cgctggaagt ggaaggtctg accaaagggt
    24481 ttgataacgg tccactgttt aaaaatctca acctgctgct ggaggtcggt gaaaaacttg
    24541 cggtactggg taccaacggc gtcggtaaat caacgctgct gaaaacgctg gtgggcgatc
    24601 tgcaaccgga cagcggcacc gtaaaatggt ctgaaaacgc gcgcattggt tactacgccc
    24661 aggaccacga atatgagttt gaaaatgatc tgaccgtgtt cgaatggatg agtcagtgga
    24721 agcaggaagg cgatgacgag caggcggtac gcagcattct cggtcgtttg ctgttcagcc
    24781 aggacgacat caaaaagcca gctaaagtgc tttccggtgg ggaaaaaggg cggatgctgt
    24841 ttggtaagtt aatgatgcag aagccgaaca ttctgatcat ggacgaaccg accaaccacc
    24901 tggatatgga atccatcgag tcactgaaca tggcgctgga actgtatcag ggcacgctga
    24961 tctttgtttc acacgaccgt gagttcgtaa gctccctggc gacccgcatt ctggaaatca
    25021 ccccggaacg cgtgatcgac tttagcggta attacgaaga ttacctgcat agtaaaggga
    25081 tcgagtaagt gattatccgt caccccgata ctgtcggggt gacggcggct gatgacaaat
    25141 gcaaaatagc ctgatgcgct ac
//
LOCUS       DAZGAN020000063        24897 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGAN020000063 DAZGAN020000000
VERSION     DAZGAN020000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.063"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..138
                     /locus_tag="ACHKYO_RS14420"
                     /old_locus_tag="pgaptmp_002884"
     CDS             <1..138
                     /locus_tag="ACHKYO_RS14420"
                     /old_locus_tag="pgaptmp_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429702.1"
                     /translation="SVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRT
                     R"
     gene            215..898
                     /locus_tag="ACHKYO_RS14425"
                     /old_locus_tag="pgaptmp_002885"
     CDS             215..898
                     /locus_tag="ACHKYO_RS14425"
                     /old_locus_tag="pgaptmp_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HGU4429703.1"
                     /translation="MSRFIQGDCVRVMATFPGNAVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            899..1120
                     /locus_tag="ACHKYO_RS14430"
                     /old_locus_tag="pgaptmp_002886"
     CDS             899..1120
                     /locus_tag="ACHKYO_RS14430"
                     /old_locus_tag="pgaptmp_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429704.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFND"
     gene            1014..1568
                     /locus_tag="ACHKYO_RS14435"
                     /old_locus_tag="pgaptmp_002887"
     CDS             1014..1568
                     /locus_tag="ACHKYO_RS14435"
                     /old_locus_tag="pgaptmp_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU4429705.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWMRALTIEKETRMYGT
                     CETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLSDPEIRTVLARLEDIPEDQ
                     RIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPE
                     CVTRRQAVVNQARILLKNNTHENG"
     gene            1614..2390
                     /locus_tag="ACHKYO_RS14440"
                     /old_locus_tag="pgaptmp_002888"
     CDS             1614..2390
                     /locus_tag="ACHKYO_RS14440"
                     /old_locus_tag="pgaptmp_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429706.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRQWFDRGVLREVVREARAEIRSMADALAKIRG"
     gene            2803..3228
                     /locus_tag="ACHKYO_RS14445"
                     /old_locus_tag="pgaptmp_002889"
     CDS             2803..3228
                     /locus_tag="ACHKYO_RS14445"
                     /old_locus_tag="pgaptmp_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4429707.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            3275..3697
                     /locus_tag="ACHKYO_RS14450"
                     /old_locus_tag="pgaptmp_002890"
     CDS             3275..3697
                     /locus_tag="ACHKYO_RS14450"
                     /old_locus_tag="pgaptmp_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU4429708.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            3694..3885
                     /locus_tag="ACHKYO_RS14455"
                     /old_locus_tag="pgaptmp_002891"
     CDS             3694..3885
                     /locus_tag="ACHKYO_RS14455"
                     /old_locus_tag="pgaptmp_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429709.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(3855..4382)
                     /locus_tag="ACHKYO_RS14460"
                     /old_locus_tag="pgaptmp_002892"
     CDS             complement(3855..4382)
                     /locus_tag="ACHKYO_RS14460"
                     /old_locus_tag="pgaptmp_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429710.1"
                     /translation="MAPDTTTGRHPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDR
                     GGATGTVAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKSHKQERNGKKRKKRKR
                     KKKKNSNRHGNHRKITSEKNIPDTSQTGRVTGDASAWPASLPAGVSLPGVFARADRRP
                     PPPALYHLFLLPCKM"
     gene            4452..4658
                     /locus_tag="ACHKYO_RS14465"
                     /old_locus_tag="pgaptmp_002893"
     CDS             4452..4658
                     /locus_tag="ACHKYO_RS14465"
                     /old_locus_tag="pgaptmp_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4429711.1"
                     /translation="MHIPQKFLPKSRQKLLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            4683..4970
                     /locus_tag="ACHKYO_RS14470"
                     /old_locus_tag="pgaptmp_002894"
     CDS             4683..4970
                     /locus_tag="ACHKYO_RS14470"
                     /old_locus_tag="pgaptmp_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429712.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWNVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            5089..5910
                     /locus_tag="ACHKYO_RS14475"
                     /old_locus_tag="pgaptmp_002895"
     CDS             5089..5910
                     /locus_tag="ACHKYO_RS14475"
                     /old_locus_tag="pgaptmp_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4429713.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGNINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(6206..6808)
                     /locus_tag="ACHKYO_RS14480"
                     /old_locus_tag="pgaptmp_002896"
     CDS             complement(6206..6808)
                     /locus_tag="ACHKYO_RS14480"
                     /old_locus_tag="pgaptmp_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HGU4429714.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICHATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYTGIKNSKGIRIPATKKSLPEINSVQNN"
     gene            7131..7514
                     /gene="traM"
                     /locus_tag="ACHKYO_RS14485"
                     /old_locus_tag="pgaptmp_002897"
     CDS             7131..7514
                     /gene="traM"
                     /locus_tag="ACHKYO_RS14485"
                     /old_locus_tag="pgaptmp_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HGU4429715.1"
                     /translation="MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLE
                     LGLRVYDAQMERKESAFNQTEFNKLLLECVVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ASMVDDIREKVSVEMDRFFPKNDDE"
     gene            7704..8390
                     /locus_tag="ACHKYO_RS14490"
                     /old_locus_tag="pgaptmp_002898"
     CDS             7704..8390
                     /locus_tag="ACHKYO_RS14490"
                     /old_locus_tag="pgaptmp_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HGU4429716.1"
                     /translation="MCALDRRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKNRKIL
                     YANGAFIELFSREDKPLSGESYIRLQVEIFLSSLELECQALGHGSAFCRRFNFHGEIY
                     QIRMENVSFYNDESVVLWQINPFPDYPFFALNQSVSNTNTSGQLTIWNDLSPGTLVVF
                     SFYMLGVGHATIARELGITDRASEDRIKPVKRKIKEFFEHFDLFRVSCIYKGEIDSLL
                     SIIREFYGVK"
     gene            8484..8711
                     /gene="traY"
                     /locus_tag="ACHKYO_RS14495"
                     /old_locus_tag="pgaptmp_002899"
     CDS             8484..8711
                     /gene="traY"
                     /locus_tag="ACHKYO_RS14495"
                     /old_locus_tag="pgaptmp_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HGU4429717.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVTEESESTFKEL"
     gene            8745..9110
                     /gene="traA"
                     /locus_tag="ACHKYO_RS14500"
                     /old_locus_tag="pgaptmp_002900"
     CDS             8745..9110
                     /gene="traA"
                     /locus_tag="ACHKYO_RS14500"
                     /old_locus_tag="pgaptmp_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HGU4429718.1"
                     /translation="MDAVLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAGSSGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFL
                     AGFAIISVFIAVGMAVVGL"
     gene            9125..9436
                     /gene="traL"
                     /locus_tag="ACHKYO_RS14505"
                     /old_locus_tag="pgaptmp_002901"
     CDS             9125..9436
                     /gene="traL"
                     /locus_tag="ACHKYO_RS14505"
                     /old_locus_tag="pgaptmp_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HGU4429719.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            9458..10024
                     /gene="traE"
                     /locus_tag="ACHKYO_RS14510"
                     /old_locus_tag="pgaptmp_002902"
     CDS             9458..10024
                     /gene="traE"
                     /locus_tag="ACHKYO_RS14510"
                     /old_locus_tag="pgaptmp_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HGU4429720.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLRYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            10011..10259
                     /locus_tag="ACHKYO_RS14515"
                     /old_locus_tag="pgaptmp_002903"
                     /pseudo
     CDS             10011..10259
                     /locus_tag="ACHKYO_RS14515"
                     /old_locus_tag="pgaptmp_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
     gene            10257..11225
                     /locus_tag="ACHKYO_RS14520"
                     /old_locus_tag="pgaptmp_002904"
                     /pseudo
     CDS             10257..11225
                     /locus_tag="ACHKYO_RS14520"
                     /old_locus_tag="pgaptmp_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
     gene            11222..11731
                     /gene="traS"
                     /locus_tag="ACHKYO_RS14525"
                     /old_locus_tag="pgaptmp_002905"
     CDS             11222..11731
                     /gene="traS"
                     /locus_tag="ACHKYO_RS14525"
                     /old_locus_tag="pgaptmp_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HGU4429721.1"
                     /translation="MITHYDIKMETQKLKDVLSVEGVNIPPLLQVIKPGGYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPEKFRDESKVISF
                     MYDKNKNYILSFLIAFSMVSFAHTLLYEFLLIALFIIFFFIYAIDINRYNLSAIASVI
                     GLFKKESVS"
     gene            11745..12476
                     /gene="traT"
                     /locus_tag="ACHKYO_RS14530"
                     /old_locus_tag="pgaptmp_002906"
     CDS             11745..12476
                     /gene="traT"
                     /locus_tag="ACHKYO_RS14530"
                     /old_locus_tag="pgaptmp_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HGU4429722.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKTTVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSSAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            12728..14881
                     /gene="traD"
                     /locus_tag="ACHKYO_RS14535"
                     /old_locus_tag="pgaptmp_002907"
     CDS             12728..14881
                     /gene="traD"
                     /locus_tag="ACHKYO_RS14535"
                     /old_locus_tag="pgaptmp_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HGU4429723.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEITGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PVSPAINDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQ
                     REQNPDIQQKMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(14890..15288)
                     /locus_tag="ACHKYO_RS14540"
                     /old_locus_tag="pgaptmp_002908"
     CDS             complement(14890..15288)
                     /locus_tag="ACHKYO_RS14540"
                     /old_locus_tag="pgaptmp_002908"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4429724.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHSGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVDGLRIEDWS"
     gene            complement(15288..15515)
                     /gene="vapB"
                     /locus_tag="ACHKYO_RS14545"
                     /old_locus_tag="pgaptmp_002909"
     CDS             complement(15288..15515)
                     /gene="vapB"
                     /locus_tag="ACHKYO_RS14545"
                     /old_locus_tag="pgaptmp_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HGU4429725.1"
                     /translation="METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHNVSADFMDNREQPGMQERESF"
     gene            15597..20867
                     /gene="traI"
                     /locus_tag="ACHKYO_RS14550"
                     /old_locus_tag="pgaptmp_002910"
     CDS             15597..20867
                     /gene="traI"
                     /locus_tag="ACHKYO_RS14550"
                     /old_locus_tag="pgaptmp_002910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HGU4429726.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMAFTPGNTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GRPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSNSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFMALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KTGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLLQRSGETPNFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWVPEHSVTQFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKSDREVMNAERLFSTARELRDV
                     VAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            20887..21633
                     /gene="traX"
                     /locus_tag="ACHKYO_RS14555"
                     /old_locus_tag="pgaptmp_002911"
     CDS             20887..21633
                     /gene="traX"
                     /locus_tag="ACHKYO_RS14555"
                     /old_locus_tag="pgaptmp_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HGU4429727.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQSWMFLVGRGAFPLFALVWGLNLSRHTHIRQEAINRLWGWAVIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRTWWRSAETMLLLAMWLPFSGTSYGIAG
                     LLMLAVSHRLYRAEDRMERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     TGKSLPRFWYGDFFPTFYACHLTVLGVLAV"
     gene            21692..22552
                     /locus_tag="ACHKYO_RS14560"
                     /old_locus_tag="pgaptmp_002912"
     CDS             21692..22552
                     /locus_tag="ACHKYO_RS14560"
                     /old_locus_tag="pgaptmp_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4429728.1"
                     /translation="MKITDHKLPEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            22655..23212
                     /locus_tag="ACHKYO_RS14565"
                     /old_locus_tag="pgaptmp_002913"
     CDS             22655..23212
                     /locus_tag="ACHKYO_RS14565"
                     /old_locus_tag="pgaptmp_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HGU4429729.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVFQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            23366..23569
                     /locus_tag="ACHKYO_RS14570"
                     /old_locus_tag="pgaptmp_002914"
     CDS             23366..23569
                     /locus_tag="ACHKYO_RS14570"
                     /old_locus_tag="pgaptmp_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429730.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            24311..24772
                     /locus_tag="ACHKYO_RS14575"
                     /old_locus_tag="pgaptmp_002915"
     CDS             24311..24772
                     /locus_tag="ACHKYO_RS14575"
                     /old_locus_tag="pgaptmp_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HGU4429731.1"
                     /translation="MKKYIPVVLFVFSCQVFSADIHGRVVRVLDGDTIDVMLSQHPVR
                     VRLVNIDAPEKKQDYGRWSEKIMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQEEARQQKRGLWADSAPVPPWVWRSRK"
BASE COUNT         6086 a         5819 c         7040 g         5952 t
ORIGIN      
        1 tcggtgacag tgtacgccag cccgtcgccc ttttctgatg aacgcccttc aagccgcttt
       61 cgcggcatat tctcgccgtc aaaaagacga cggctgcgat attccacggt cggcctgacc
      121 cgttaccgga cgcggtgaac agcccacagg ccgcggggaa cgggcgccac aggggcgccc
      181 tcccggtgcc ctctgtaaga gaaggagtgc tgttatgtcc cgttttatcc agggtgactg
      241 cgtgcgtgtt atggccacgt ttccgggcaa tgctgtcgat ttcatcctca ccgacccgcc
      301 atatctcgtc ggtttccgtg accgtcaggg gcgtaccatc gccggcgata aaaccgatga
      361 atggctgcaa ccggcctgta atgaaatgta ccgcgtgctg aaaaaagatg cgctgatggt
      421 gagcttctat ggctggaacc gcgtcgatcg ctttatggcc gcctggaaaa atgcgggatt
      481 cagcgtggtt ggccacctgg tgtttaccaa aacctacaca tcgaaggccg catatgtggg
      541 ctatcgccac gaatgcgctt acatcctggc aaaagggcgt ccggcgctgc ctcagaagcc
      601 attgcccgac gtgctgggct ggaaatattc aggcaatcgt catcacccga cggaaaagcc
      661 tgttaccagc ctgcaaccac tgattgagag cttcacacac ccgaacgcga ttgtgctgga
      721 cccgtttgca ggcagcggct caacctgtgt ggccgccctt cagtccggac gccggtatat
      781 cggtatcgag ctgcttgaac agtatcaccg tgccggacag caacgtctgg ctgccgtaca
      841 gcgggccatg cagcaggggg ccgcaaatga tgactggttt atgccggagg ctgcgtaaat
      901 gaactatgcc ggacatgaaa aacttcgcgc cgacgtggcg gaggtggcca acaccatgtg
      961 tgaccttcgg gccaggctga acgatatgga gcatcgctgt cgctttgatt ccgatgtgct
     1021 tgttgaacgc ttggcacgcc agacgctgta tcgcgccaat cgcctgttca tggaggcgta
     1081 taccgaaatt ctggaactgg atgcgtgctt taacgattga gaaggagacg agaatgtacg
     1141 gaacatgcga aacgctatgc cgggagctgg cagcaaagta tccgggatat acaccgctga
     1201 tgctggttat ctggtccccg gaagagattc aggccctcgc tgacggaatg gatatttccc
     1261 tgtccgatcc tgaaatcagg accgtcctgg cgcgcctgga ggacatcccg gaagaccagc
     1321 gaattgagtc cggtatttct tctgccgcgg cgatggagat tatcagcaac gtgagcgaaa
     1381 accgccaggt gaccgtcccg gctgaactgc tggcgtccct gattcagacc gccgaacagg
     1441 cactgtggaa acgtgaatgg gccgcccgtg attacggcct cgccgtcccg gaatgcgtta
     1501 cccgccgtca ggcggtggtt aatcaggccc gcatcctgct gaaaaacaac acacacgaaa
     1561 acggctgatg ttatcgccgc ctgcgggcgg cgatacaggg agaacgatta atcatgaacg
     1621 aaaacacaac gctgaacgca ctgatttgtc gtcacgcccg taacctgctg ctggcgcagg
     1681 gctggccgga agagacggat gtcgaccagc gtaacccgaa gtatccgggc tggatcagca
     1741 tttatgttct gctggatgcg ccccggctgg cgacgttact cgttaaccgc cacggcggcg
     1801 tactgccgcc gcacctggcc tccgccattc agaaactgac cggaaccgga gcggaacttg
     1861 ttctgtccgg cagtcagtgg cagtcgctgc cggtgcttcc ggcagacgga acgcaggtgt
     1921 cttttccgta tgccggtgaa tggctgaccg aagacgaaat cagggctgta cttgcggcgg
     1981 tgcgcgatgc ggtacgcagt gtcagttgcc gggtggcaga agatacgcgg cgtatccggg
     2041 cggcgctgac caccaccggt cagacgttac tgacccgcca gacgcgccgc tttcgcctgg
     2101 tcgtgaagga aagcgatcac ccctgctggc tcgatgagga cgacgaaaac ctgcccgtgg
     2161 tgctcgatgc catcctgaac cggggcgcac gtttttcggc ggtggaaatg tatctggtca
     2221 gcgattgtat tgaacatatc ctgtccagtg ggctggcctg cgatgtgctg cgtataccgg
     2281 atgaaccgcc ccgccagtgg tttgaccgtg gtgttctgcg ggaagttgtc cgggaagccc
     2341 gggccgaaat ccgcagcatg gcggatgccc tggcaaaaat caggggctga tgatggcggg
     2401 gaaaatcccc gctttttttc tgccggtccc cggacagagc acaaaggaag tcgccgccgg
     2461 ctccgcttta tccggccatg caggcatggc cttgtgggtt ttcagttatg tggccgccgt
     2521 gtcgtgtgcg ggcggtgccg tgcctccatc ttagccgggc tggcggggat gcaagggtac
     2581 gcttcgccgc tgcggtcacc cggtccttcc ttcccgtccg ccgtgatttt ccggtcgttc
     2641 acccgctcag gtttcgcggt ctcaccctcc aactcccgcc agccgtgtgc gtcaggctgc
     2701 ggcttccttt gcgccctgtg catcccgcct tatcgcccgg cttttatgga ggcacggcac
     2761 cgcccggtgt cgcggaatat gtgaataacg gaggaaaaca tcatgcaata tgcgaaacct
     2821 gtcactctga acgttgaaga gtgcgaccgt ctctcctttc tgccttacct gtttggcaat
     2881 gattttctgt atgccgaggc gtatgtgtac gcgctggcgc aaaaaatgat gccggaatat
     2941 cagggaggct tctggcactt catccgcctg ccggacggtg gcggttacat gatgccggat
     3001 ggcgaccgtt ttcatctggt caacggtgaa aactggtttg accgcacggt cagcgccgat
     3061 gccgcaggca tcattctgac ttcccttgtg attaaccgcc agttgtggct gtaccacgac
     3121 agcggggatg caggactgac ccagctttac cggatgcgcg atgcgcagtt gtggcgtcac
     3181 atcgaatttc accccgaatg caacgcgatt tacgcggcac tggactgatt aacggacggg
     3241 gcggcaatgc cgcccctgta aaggagacga gaacatgtac tgtactgtta aagaaatcat
     3301 ccgcgatgta ctggatacag acgtgccgga cagtgaatgc gtttttgccg tggtgctgac
     3361 ccgtggggat gtgcgccaca tagcccagga ctggaacctg tcagacgatg agctggaaac
     3421 cgtcatgcag cggctggacg atgcctttga atatggtgcg gatgtcagca ttgttcacga
     3481 cgttgttcgt gagctgatgg aagaaaagcg tgccagccgt caggtgacag ttccggcggt
     3541 gatgctggaa aaagtgatgg cgctggcagg cagtgaaatg aagcgcctgt atgccgtcgg
     3601 gagtgagaac gggggcgacg gtgatgcgtt cgtcagggag gaacgcgaag caatggacgt
     3661 tgtgttacag gcgctggacg gggagcacat gtcatgaata tcagcacaga aacccgcgaa
     3721 attctgcgca attacaaagc cgtgattaat gcgcgacgtc gtgaaatggg gcagaaaccg
     3781 ctcaccaccg cgcagattgt tgatgaaatc tgcgattttg tggcgaatca gcaggctgtt
     3841 ttcctgggcg gtcactacat cttacagggc agcagaaaca ggtgatacag ggcaggcggt
     3901 ggaggccgcc tgtcagcccg cgcgaaaacc cctggcaggc tgacgcctgc cggaagggaa
     3961 gcgggccacg ctgacgcgtc gcccgttacc cttcctgttt gtgatgtgtc cggaatgttt
     4021 ttttctgatg ttatttttct gtggttaccg tgcctgttac tgttcttctt ctttttcctc
     4081 ttcctcttct tcctcttctt tccgttcctc tcctgcttat ggcttttccg ggcgtccccc
     4141 tggcggggcg ggctgtcgtg aacggagcca gcgaagctgt ctccgccctg gcgggcttcc
     4201 atccctgacg cgaaaccctg gcttgcagcg accgttccgg tcgcgccgcc tctgtcgcgc
     4261 cgtggcctgc gctcccggca tcccggcaaa aggccgccgg gatgtaaggg gcaacgccgt
     4321 ttcgcaaacc gcgcttcacg tcgttgcggc gggtgcctgc ccgttgttgt gtccggtgcc
     4381 attcacctgt ttaagcccct gtagccgggg gctgatacag ccacaaccgt ctccgccagt
     4441 ccaggggtga aatgcacatc ccgcaaaagt ttttgcccaa aagcaggcaa aaacttttgc
     4501 ggtctgcccc cggacagtcg tttccggccg tggcagtgat gccccttgcg gcgaaggttc
     4561 tcaaaaaggt gatggtgaat accggaggga acaaaaggca ggtccccgca aaacgtcaaa
     4621 accgcggctc ccgaaactca aaagatgatg ggggctgaaa accagagaga ggagaaagca
     4681 ggatgagtac ccgaaatatc cacgttaaca ccgcgtcgta cacgcttctt gttgccggga
     4741 agaaaaaaaa caccggggaa gagtggaacg tcctggaatt cagcagcctg actgagctga
     4801 aaaaatatcg caaaagccac ccggaaaaga tggctttcag ctatagctac gcgctcagcc
     4861 ggggtgtgga tacgcagttc cgccatatca acattgcgga agccgatcac ttcaaacagt
     4921 tcctgcgtca gattaagcgt gccggcctcg atatccgggc gatctgctga ccggatgtga
     4981 tgaatatccc tcctgattgt tgcaatcttg ttaatggtga ccagggggga tgatattaag
     5041 cggaaaaatt ttttattaaa tttattcttt cataaaggag tagctgttat gcgattagct
     5101 tcccgttttg gtcggtataa ttccatccgc cgtgaacgtc ctttaacgga tgatgaatta
     5161 atgcagttcg tgccttcggt attttccggt gataaacatg agtcccggag tgaacgttat
     5221 acgtatattc caacaatcaa tatcatcaat aagttacgtg atgaaggttt ccagccattc
     5281 tttgcctgtc agagtcgggt tcgtgatttg ggacgtcgcg aatacagtaa acatatgtta
     5341 cgtcttcgca gggaagggaa tattaacggg caggaagttc ctgaaattat cttgcttaat
     5401 tcacatgatg gttcatccag ttatcagatg atccccggaa tttttcgttt tgtctgcaca
     5461 aatggcctgg tgtgcggaaa taattttggc gaaatccgcg ttccacataa aggtgatatt
     5521 gtcgggcagg ttatagaggg cgcgtatgaa gtgctcggtg tctttgataa ggtcactgat
     5581 aatatggagg cgatgaaaga aattcatctt aacagtgacg agcaacattt atttggcaga
     5641 gctgcactga tggtcaggta tgaagatgaa aataaaacgc cagtgacgcc tgaacaaata
     5701 attactcccc gtcgttggga ggataaacag aacgatctct ggacaacctg gcagcgggtt
     5761 caggagaata tgataaaagg tggattatcg gggcgaagtg cctccgggaa aaataccagg
     5821 acaagagcca ttacaggtat tgatggtgat ataagaatca acaaggcgtt gtgggtgatt
     5881 gccgaacagt tcagaaaatg gaagtcatga taatataagt aaagccccga aaaaatttcg
     5941 gggctttact tatattatca ttcttcggca tcgctgtgtc gtctgatata cctcaatttc
     6001 atcaactggc ttttcggttg tctgtaaata ttgtgcccgc gaaaaatggt ctggcaggag
     6061 gctgcgcagt taccgggtat atataagatg ggttaccgct tcgcggggct tcggccagtc
     6121 agtattgtca ctcatatgat atttttgtgt gtggccttcc atgccgcttt tgcggcataa
     6181 ccggtatctg acaatgtctg taagattaat tgttctgcac gctgttaatt tccggaagtg
     6241 atttcttcgt ggccggaatc cggatacctt tactgttttt tatcccggta taaatccggt
     6301 aaacatctga tgcgtaggca agacgtctct ggttctggcg ttcggtcttc ctgaatccag
     6361 cattgtatgc accaacggcc tcccaggaga cgccccattt tttaaaggct attgccagat
     6421 aataagcacc ggtataaatg ttcatgcagg gatctgttgt cagatgttcc ggcttaattc
     6481 catagcgggc cagttcgtta aaatgctggg aatctacctg catcagtccg ctgccatatc
     6541 ccgttaccgg attaataccg atggcattaa cccggtaacg ggattctttc catgatattg
     6601 ctctcagtaa atcaggatct attttgtaat cccggcctgc aagatcaaag caatcagtgg
     6661 catggcagat ctcatttata aacatcaggc agatggctaa catccatttt ttcatttttc
     6721 cacctctggt gactttatca gtaaataatt taacccactc cacaaaaagg ctcaacaggt
     6781 tggtggttct caccaccaaa agcaccacac cccacgcaaa aacaagtttt tgctgatttg
     6841 ttttttaaat cattagttta tgttctaaat aatgtatttt aatttatttt acattattaa
     6901 atagggtcgt tgacgcccct atggcgaccc tatgacgacc cttgttaggg tcagtgctga
     6961 caattttcat gaaaaacatt tggatatatt agggccgccc ctagcagcgc cgtgcgtttt
     7021 tttatagtat gcccctaggg ggcgctgcta ggggtgctct tcttttttca gaccatacta
     7081 tgagtagatg aaataaaact cttatttcag aatctaaaag gtactgtctt atggccagag
     7141 taaatctgta tatcagtaat gaggttcatg aaaaaattaa catgattgtt gaaaagcgtc
     7201 gtcaggaggg agcaagagat aaagatataa gcctttcagg aactgcttca atgcttcttg
     7261 aattagggct ccgtgtatat gacgctcaga tggagcgtaa agagtctgca tttaaccaga
     7321 cagagtttaa taaacttctt cttgaatgtg ttgtaaaaac acagtcaacg gtggcaaaga
     7381 ttttaggtat tgaatctctc agtcctcatg tatccggaaa cccgaagttt gaatatgcca
     7441 gtatggttga cgatatcaga gagaaagtgt ctgttgagat ggaccggttt ttcccaaaaa
     7501 atgatgatga ataaatgaaa atgaagtttg agttggttca aatataggag tttttatgat
     7561 tcaaaaagat gacaatacgt cagataatta gtatattaat tacgtggtta atgccacgtt
     7621 aaaaattgaa actgaaaatc gccgatgcag ggagctgtaa ctccctgcat cgactgtcca
     7681 tagaatcctt agtggaggtt cctatgtgtg cgctggatcg tagagaaagg ccacttaaca
     7741 gtcaatctgt aaataaatac atccttaacg ttcagaatat ctacagaaat tctcccgttc
     7801 cggtttgtgt ccgtaacaaa aaccggaaaa tcctttatgc caatggggct tttattgaac
     7861 tcttttccag agaagataaa cctttatccg gagagagtta tatacgtctg caggttgaaa
     7921 tttttctttc atcacttgaa ctggaatgcc aggctcttgg acatggctct gcattttgtc
     7981 gtcgttttaa ttttcatggc gaaatctatc agataaggat ggagaatgtt tctttttata
     8041 atgacgaatc tgttgtttta tggcaaatta atccgtttcc tgattatcca ttttttgcgt
     8101 taaatcagag tgtaagtaat acaaatactt ctggtcaatt aacgatatgg aatgatcttt
     8161 ctccaggaac attggttgtt ttctcttttt atatgctggg tgttggtcac gcaacaattg
     8221 ccagagaatt gggtattaca gacagagcat ctgaggatcg aattaaacca gttaaacgga
     8281 aaataaaaga gttttttgaa cactttgatt tattcagagt gtcatgtatc tataaaggag
     8341 aaatagattc gctattaagt ataattcgtg aattttatgg tgttaaataa atgttaaata
     8401 aggcgcgcca attttggcgt gttttttttc gccgatttct gtgcaatcat aattttatta
     8461 gtcgggaaac agaggtgaat ttagtgagga ggcgtaacgc gagaggcggt ataagcagaa
     8521 cggtctcggt gtatctcgat gaagatacaa ataaccgact tattaaggcc aaagatcgta
     8581 gtggaagaag taaaacgata gaagtccaga tccgtctcag agatcatctg aaacggtttc
     8641 ctgatttcta taacgaagaa atattcaggg aggtgaccga agagagcgag tcaacattta
     8701 aagaacttta gtttttatct atcaatatta aacagaggaa tcatatggac gccgttttaa
     8761 gtgttcaggg tgcttctgcg cccgtcaaaa agaaatcgtt tttttccaaa ttcactcgtc
     8821 tgaatatgct tcgcctggct cgcgcagtga tcccggctgc tgttctgatg atgttcttcc
     8881 cgcagctggc gatggccgcc ggcagcagtg gtcaggacct gatggcaagc ggtaacacca
     8941 cggttaaggc gaccttcggt aaggactcca gtgttgttaa atgggttgtt ctggctgaag
     9001 ttctggtcgg tgctgtcatg tacatgatga ccaaaaacgt caagttcctg gccggttttg
     9061 ccatcatctc tgtatttatt gctgtgggta tggccgtcgt tggcctctga caggaaataa
     9121 aacgatgtcg ggagacgaga ataaacttaa gaaatatcgt ttcccggaaa cactgaccaa
     9181 ccagagccgc tggtttggcc tgccactgga tgaactgatc cccgcagcaa tctgtattgg
     9241 ctggggtatc acaacatcga aatatctgtt cggtattggt gcagcggttc tggtttattt
     9301 cgggattaaa aaactgaaaa aagggcgggg cagttcctgg ttacgtgacc tgatttactg
     9361 gtatatgcca acagccctgc tgcgtggtat ttttcataat gttcccgatt cgtgtttccg
     9421 gcagtggatt aaatagaact gataccagga ttgttatatg gaacacggtg cccgtttaag
     9481 taccagtcgt gtaatggcca tcgcctttat atttatgtca gtgcttattg ttctcagcct
     9541 ctctgttaac gtcattcagg gggtgaataa ctaccgtctt cagaatgagc aacgcactgc
     9601 cgtgacgcca atggcattta atgccccctt tgccgtgtca cagaacagtg ccgacgcctc
     9661 ttatttacag cagatggcgc tgtcatttat tgccctccgt ctgaatgttt catcagaaac
     9721 cgtcgatgcc tcacatcagg cgcttctgcg atatatccgc ccgggcgcac agaaccagat
     9781 gaaagttatt ctggctgaag aagcgaagcg tattaaaaac gataacgtga actcagcctt
     9841 tttccagacc agtgttcgtg tctggcctca gtatggccgt gtggaaattc gaggtgtgct
     9901 taaaacctgg attggtgatt caaaaccttt cactgatatc aaacattaca tccttattct
     9961 gaagcgggaa aacggggtga cctggctgga taatttcggg gaaacagacg atgagaaaaa
    10021 ataatacggc aataatattc ggcagcctgt ttttttcctg cagcgtgatg gccgcaaacg
    10081 gtacgctggc ccccaccgtg gtgccaatgg tgaacggtgg tcaggccagt attgccatca
    10141 gcaataccag cccgaatctg tttaccgttc ccggtgaccg gattatcgcc gtgaacagtc
    10201 tggatggtgc cctgaccaat aatgagcaga ccgcctccgg cggtgtggtg gttgcatcag
    10261 ctggattgta tggtgatgct catgccggat tagatgcaaa gcccagcctt tttggtgtgc
    10321 gtgttgatgt tggcgctaag gttggtgggc gagccagtat tgacggtagt gatctggact
    10381 cacatgaagc cagtagtggc agcagggttt cccatgatgc tcgccatgat attgacgcta
    10441 gagccagtaa ggactttaaa gaggccagcg attactttac cagtcgcaaa gtcagtgaat
    10501 ccggcagcca tactgacaat aatgccgatt cccgtgtgga ccagctgtct gcagccctga
    10561 actcagcaaa acagagttac gaccagtaca cgacgaatat gacccgcagc catgaatatg
    10621 ctgaaatggc atcccgtact gaaagcatga gtgggcagat gagtgaagac ctatcgcaac
    10681 agtttgcaca gtatgtgatg aaacacgctc cgcaggatgc agaagctatt ctgacaaata
    10741 ccagttcgcc ggagattgca gaacgccgtc gggcaatggc gtggtctttt gtgcaggaac
    10801 aggtgcagcc tggtgttgat aatgcctggc gtgaatcccg tggtgatatt ggtaaaggaa
    10861 tggagagcgt accttcgggt ggtggcagcc aggatattat tgctgaccat cagggacatc
    10921 aggccattat tgagcaaaga acgcaggaca gtaatatccg aaatgatgta aaacatcagg
    10981 ttgataatat ggtcacagaa taccgaggca atatcgggga tactcagagc agcatacatg
    11041 gcgaagaaaa tattgtagga agacagtatt ctgaactaca aaatcaccat aaaacagaag
    11101 cattatctca gaataataaa tatgatgaag agcgagaaag tcaaaagata atacctgagc
    11161 catcaggaga ggctgtgaaa aaaatgattg atggtaaaaa ggaacgactg aaggggccat
    11221 tatgatcact cactatgata taaaaatgga gacacagaaa ctaaaggatg tactctctgt
    11281 agagggggtt aatatcccgc cattattaca ggtcataaag ccaggggggt atgtgttttt
    11341 atgggtttta ttatggccaa catttcttcg tttgctggca gataaagttg atatccgaga
    11401 tgccggattt gatatatgtt ttagtggcgt aatgggtttt atcctttttg ttgccattac
    11461 taatggaatg atgttgtatc ttgctatccc tgaaaaattc agagatgagt cgaaagttat
    11521 atcttttatg tatgataaga acaaaaatta tatcttgtca tttttgattg ctttttcaat
    11581 ggtttcattt gcccatacac tcttatatga gtttttatta attgctttgt ttataatctt
    11641 tttctttatt tatgctatag acataaatcg ttataatctc tcagctatag cctcagtaat
    11701 agggctgttc aaaaaagagt ctgttagtta aaaggatatt ttggatgaaa acaaaaaaat
    11761 tgatgatggt tgcactggtc agttccactc tggccctttc agggtgtggt gcgatgagca
    11821 cagcaatcaa gaagcgtaac cttgaggtga agacccagat gagtgagacc atctggcttg
    11881 aacccgccag cgaacgcacg gtatttctgc agatcaaaaa cacgtctgat aaagacatga
    11941 gtgggctgca gggcaaaatt gctgatgctg tgaaagcaaa agggtatcag gtggtgactt
    12001 ctccggataa agcctactac tggattcagg cgaatgtgct gaaggccgat aagatggatc
    12061 tgcgggagtc tcagggatgg ctgaaccgtg gttatgaagg cgcagctgtt ggtgcagcgt
    12121 taggtgccgg tattaccgga tataattcca attctgccgg tgccacactc ggtgtgggcc
    12181 ttgctgctgg tctggtgggt atggctgcag atgcgatggt ggaagatgtg aactatacca
    12241 tgatcacgga tgtgcagatt gcagagcgta ctaagacaac ggtgacaacg gataatgttg
    12301 ccgccctgcg tcagggcaca tcaggtgcga aaattcagac cagtactgaa acaggtaacc
    12361 agcataaata tcagacccgt gtggtctcca gtgcgaacaa ggttaacctg aaatttgaag
    12421 aggcgaagcc tgttctcgaa gaccagctgg ccaaatcaat cgcaaatatt ctctgattat
    12481 cttccggagg ctggtctgac cggcctcctg actgacgcct cgctttgctc gttgtccaac
    12541 cccggaaaga aataaaataa attttatttc tccccggcgt ttccgaaata aatatgaaaa
    12601 cactgagcgg ttgtgcaaat tctttgtgcg accgtgaagt gttgccggcg aagccggaat
    12661 ttaacggtta ttaatctttt catcattaat aatcagttcg actgactttt ttcttcggaa
    12721 tatcatcatg agttttaacg caaaggatat gacccagggc ggtcagattg cgtccatgcg
    12781 tatccgcatg ttcagccaga tcgccaatat catgctttac tgcctgttta tttttttctg
    12841 gatactcgtt ggtctggttt tatgggtaaa aataagctgg cagacgtttg tgaacggctg
    12901 tatttactgg tggtgtacca cgctggaagg catgcgggat ttaatcaagt cccagccggt
    12961 atatgagatc cagtattacg gcaaaacctt ccggatgaat gctgctcagg tactgcatga
    13021 taaatatatg atctggtgcg gagagcagct atggtccgca ttcgttctgg ccacagttgt
    13081 ggcactggtt atttgcctga ttaccttctt tgttgtctcc tggattctgg ggcgtcaggg
    13141 taaacaacag agcgaaaatg aaatcacagg tggtcgtcag ctgactgaca atccgaaaga
    13201 ggtggccagg atgctgaaaa aagatggcaa ggattccgat atccggattg gcgatctgcc
    13261 gattatccgg gattctgaaa tccagaactt ctgtctgcac ggcactgtcg gggccggtaa
    13321 gtcggaggtt atccgtcgtc tggccaacta cgcccgtcag cgtggtgata tggtggtgat
    13381 ttatgaccgt tcgggggaat ttgttaaaag ttactatgat ccctccatcg ataaaatcct
    13441 gaatccgctg gatgcacgct gtgctgcctg ggatttgtgg aaggagtgcc tgacacagcc
    13501 agattttgat aataccgcaa atactctgat cccgatgggg acaaaagagg acccgttctg
    13561 gcagggttca ggacgtacca tttttgcgga agcggcatac ctgatgcgta atgaccccaa
    13621 ccgcagctac agcaaactgg tggacacact gctttccatc aaaattgaaa agctgcgtac
    13681 cttcctgcgt aattcaccgg cggccaacct ggtggaagag aaaatcgaga aaacggccat
    13741 atccatccgt gctgtgctga ccaactacgt gaaagccatc cgttacctgc aggggattga
    13801 gcataacggt gagcccttca ccatccgtga ctggatgcgt ggtgtccggg aagatcagaa
    13861 aaacggctgg ctgtttattt cgtcgaacgc cgacacccat gcctccctga agccggtgat
    13921 ctccatgtgg ctgtccattg ctattcgtgg tctgctggca atgggagaaa accgtaaccg
    13981 tcgtgtgtgg tttttctgtg acgagttacc cacgttacac aaactgccgg acctggtgga
    14041 gatcctgccg gaagcccgta agttcggtgg ctgttatgtg tttggtatcc agtcctatgc
    14101 ccagctggaa gatatctacg gtgagaaagc ggctgccacg ctgtttgacg tcatgaacac
    14161 ccgtgccttt ttccgttctc ccagccataa gattgcagag ttcgctgccg gtgaaattgg
    14221 tgagaaagag cacctgaaag ccagcgagca gtattcctac ggtgctgatc cggtacgtga
    14281 cggggtatcg accggtaagg atatggagcg tcagacgctg gtcagttatt ccgacattca
    14341 gtctctgccg gatctgacct gttatgtcac cctgcccgga ccgtatccgg cagtaaaact
    14401 ctccctgaaa tatcagacac gaccgaaggt cgctccggag tttattccgc gtgacatcaa
    14461 cccggaaatg gagaaccgtc tgagtgccgt acttgccgca agggaagcag aaggtcgtca
    14521 gatggccagc ctcttcgaac cggatgtccc ggaggttgtt tccggagaag acgtgactca
    14581 ggctgaacag ccgcaacagc cggtgtctcc tgccatcaac gataagaagt cagatgcagg
    14641 tgtgaatgtt ccggcagggg ggatcgagca ggagctgaaa atgaaaccgg aagaagagat
    14701 ggaacagcaa ctgccacccg ggatcagtga atccggtgaa gtggtggata tggccgttta
    14761 tgaggcatgg caacgggagc agaatccgga tatccagcag aagatgcagc gtcgtgaaga
    14821 ggtgaacatc aatgtgcacc gggagcgcgg ggaggatgtt gagccgggag atgatttctg
    14881 atgaacaggt cagctccagt cttcaattct cagaccgtcc acacgttcaa attcccgggt
    14941 gttattagtc acaataatca gtccccgact gcgggcatga cctgcgatca tttgatcaaa
    15001 tggcccgaca gggcgtccct gacgggcaag ttctgctctt atctggccgc tgtgtgtggc
    15061 tgcggcagcg tcgtaatcca gaacatcaat gcgggaaaca aatccctcga tcacagcgag
    15121 attacgttca ggcatctggc ttttttctgc accatatatc agctccatca gggtgaccga
    15181 actgatacac attctcccct ggttcaggtt aaaacgctcc ctgacgctgg cgggtttgtt
    15241 ctttatcgtg aaaatgcaga tgttggtatc gagcataaac ttcagcatca gaatgactcc
    15301 ctttcctgca tgccgggctg ttccctgtta tccataaaat cggcgctgac gttgtgtccg
    15361 tcgaaccatt cgtcccatgt ctctccggct ggcgtaatga ttctggttcg tccgacagca
    15421 atcacttcaa cgcgttttac gttttccggc aatgcaaccg cttttggcag tctgaccgcc
    15481 tggctgcggt tgctgaaaaa tacggtggtt tccattattt gcctccttat gtggatatgt
    15541 cttgtgtata tccattgtgg gattgatacc gggatatgtc aaagggatat acgtttatga
    15601 tgagtatcgc gcaggtcaga tcggccggaa gtgccggtaa ctattatacc gacaaggata
    15661 attactatgt gctgggcagc atgggagaac gctgggccgg caggggggct gaacagctgg
    15721 ggctgcaggg cagcgtcgat aaggatgttt ttacccgtct tctggagggg aagctgccgg
    15781 acggagcgga tttaagccgc atgcaggatg gcagtaataa gcatcgtccc ggctacgacc
    15841 tgaccttctc cgcccccaaa agtgtctccg tgatggccat gttaggcggt gataaacgtc
    15901 ttattgatgc acataaccag gccgtggatt ttgctgtccg tcaggtggag gcgctggctt
    15961 ccacacgggt tatgacggac ggacagtcag aaacggtgct gaccggtaat ctggtgatgg
    16021 cactgtttaa ccacgacacc agtcgcgatc aggaaccaca gttacacaca catgcggtag
    16081 tggccaatgt cacgcagcat aacggcgagt ggaagacact gagcagtgac aaagtgggga
    16141 aaacggggtt cattgagaat gtgtacgcta atcagattgc cttcggcagg ctctaccggg
    16201 aaaaactgaa agagcaggtt gaggcgctgg gctatgaaac ggaagtggtt ggtaagcacg
    16261 gtatgtggga gatgccgggc gtaccggtgg aggccttctc cggacgctca caggctatcc
    16321 gggaggccgt cggggaagac gcctcgctga aatcccggga tgtggcggca ctggatacgc
    16381 gaaaatccaa acagcacgtc gatcctgaag tcagaatggc tgaatggatg cagacgctga
    16441 aggaaaccgg gttcgacatc cgggcgtatc gtgacgcggc ggaccaacgg gcggagaccc
    16501 gcacgcagac gcccggaccc gcttcacagg acgggccgga tgtgcagcag gcggtgacac
    16561 aggccattgc cggattaagt gaacgcaaag tgcagtttac gtacacggac gtgctggcca
    16621 gaacggtcgg catactgccg ccggaaaatg gtgtgattga acgggcacgc gccggtatcg
    16681 atgaggccat cagccgtgag cagcttatcc ccctcgatcg tgagaagggg ctgttcacgt
    16741 ccgggattca tgtgctcgat gagctgtcag tccgggcact cagtcgtgac atcatgaaac
    16801 agaaccgggt gaccgtacat ccggagaaaa gtgtcccccg gacggccggt tacagcgatg
    16861 cggtcagtgt tctggcacag gatcgcccgt cgctggctat tgtgtccggg cagggcggtg
    16921 cagccgggca gcgtgagcgg gtggcggagc tggtcatgat ggcccgggag caggggcggg
    16981 aggtgcagat tatcgccgct gaccgtcgct cgcagatgaa cctgaagcag gatgaacggc
    17041 tgtccggtga gctgataacc ggacgtcgtc agctgcagga gggcatggcc ttcacgccgg
    17101 gcaatactgt tatcgttgac cagggcgaaa aactctccct gaaagagacg ttaaccctgc
    17161 tggacggtgc cgcacgtcat aacgtacagg tcctgataac cgacagcggg cagcgaaccg
    17221 gtacaggcag tgcactgatg gccatgaaag atgctggggt gaacacatat cgctggcagg
    17281 ggggagaaca gcggccggcc accatcatca gtgaaccgga ccgtaatgtc cgctatgccc
    17341 ggctggcagg agattttgct gccagcgtga aagccggaga agagagcgtg gcacaggtca
    17401 gcggggtacg ggaacaggcc atactgacac aggccattcg cagtgagctg aaaacacagg
    17461 gcgtgctcgg acgcccggag gtaaccatga ccgccctgtc gccggtctgg ctggacagcc
    17521 ggagccgtta tctgcgggat atgtaccgtc cggggatggt gatggagcag tggaacccgg
    17581 agacacgcag tcatgaccgc tatgttatcg accgggtgac ggcgcagagt aacagcctga
    17641 ccctgcggga tgcgcagggt gaaacgcagg tggtgcgtat ttcctccctg gacagcagct
    17701 ggtcgctgtt ccggccggaa aaaatgccgg tggcagacgg cgagcgactg agggtgacag
    17761 ggaaaattcc cggactccgc gtctccggcg gtgaccgcct gcaggtggca tccgtcagtg
    17821 aagatgcgat gacggttgtt gtgccggggc gggctgaacc ggcctccctg cctgtgagcg
    17881 attcaccatt catggcactg aagctagaga acggctgggt ggaaacgccc gggcattccg
    17941 tcagcgacag tgcgaaggtt tttgcctccg tcacacagat ggcaatggac aacgccaccc
    18001 tgaacggtct ggcccgcagc ggtcgtgatg tccggctgta ttcctcactg gatgaaaccc
    18061 gtactgcgga aaaacttgcc cgccatccct cctttacggt ggtttctgag cagataaagg
    18121 cgcgtgccgg tgagacattg ctggaaaccg ctatcagtct gcagaaaacc gggcttcaca
    18181 cgccggcaca gcaggctatt catctggcgc ttcctgtggt ggaaagtaaa aacctggcct
    18241 tcagcatggt ggacctgctg acagaggcga agtcgtttgc tgcagaagga accagtttta
    18301 ctgaactggg aggggaaatc aatgcgcaga taaaacgcgg tgatttactg tatgtggatg
    18361 tggcaaaagg ctatggcaca ggcctgctgg tttcccgtgc gtcgtatgag gcagaaaaga
    18421 gcattcttcg ccatattctc gaaggtaagg aggcggtcac gccactgatg gagagagtac
    18481 ctggcgaact catggagaaa ctgacatcag gacagcgtgc cgctacccgc atgatactgg
    18541 aaacgtccga ccgtttcacg gtggtgcagg gctatgccgg tgtgggtaag accacacagt
    18601 tccgggcggt gatgtcagcc gtgaacatgc tgccggagag tgaacgtccc cgtgtcgtgg
    18661 ggctggggcc cacgcaccgt gcggtcggtg agatgcgcag cgccggcgtg gatgcacaga
    18721 cgctggcgtc ctttctgcat gacacgcagc tgctgcagcg cagcggagaa acgccgaatt
    18781 tcagcaacac gctgttcctg ctcgatgaga gctctatggt gggcaatacc gacatggcac
    18841 gggcatacgc cctgattgcg gccggtggcg gtcgtgccgt ggccagtggt gacacggacc
    18901 agttgcaggc catcgcgccc ggtcagcctt tccgtctcca gcagacgcgc agtgctgccg
    18961 atgtggccat catgaaggag attgtgcgcc agacgccgga gctgcgggag gcggtataca
    19021 gcctgattaa ccgggatgtg gaaagggcac tgtccgggct tgagagtgtg aaaccgtctc
    19081 aggtgccacg tcaggagggc gcatgggtac cggagcactc cgtgacgcag ttcagtcaca
    19141 gccaggaagc gaaactggca gaagcgcagc agaaggcgat gctgaaaggt gaggcttttc
    19201 cggatattcc catgacactg tatgaagcca ttgtccgcga ttataccggc aggacgccgg
    19261 aagcacggga gcagacgctg attgtcacgc atctgaatga ggaccggcgc gtactgaaca
    19321 gcatgattca tgatgcacgg gaaaaggccg gtgagctggg aaaagagcag gtcatggtgc
    19381 ctgtcctgaa cacagcgaat atacgcgacg gggagttgcg tcgtctctcc acctgggaga
    19441 cacatcggga cgcacttgcc ctggtggata atgtgtatca ccggattgcc ggtatcagca
    19501 aggatgacgg gctgataacc ctgcaggatg cggaaggtaa cacgcggctg atttcgcccc
    19561 gggaggcggt ggctgaaggc gtcacactgt acactccgga caccatccgg gtgggaaccg
    19621 gtgaccggat gcgcttcacg aagagtgacc gggagcgcgg ttatgtggcc aacagcgtct
    19681 ggacggtgac agcggtttcc ggtgacagtg tcacgctgtc ggacggacag cagacccggg
    19741 tgattcgccc cggtcaggag cgggcagagc aacatattga cctggcctat gccatcaccg
    19801 cccacggtgc gcagggggca agtgaaacct ttgccattgc gcttgaaggc acggaaggta
    19861 accggaaaca gatggccggc tttgagtcag cctacgtggc cctgtcgcgt atgaagcagc
    19921 atgtgcaggt gtataccgat aaccgtcagg gctggacgga tgccattaac aatgccgtac
    19981 agaaaggaac tgcccacgat gtgctagagc cgaaatcgga ccgggaggtc atgaatgcag
    20041 agcggctgtt cagtacggcg cgggagctgc gggacgtggt ggccggccgt gccgttctcc
    20101 gtcaggcagg gctggccggg ggagacagtc ctgcacggtt tattgcaccc gggcgcaaat
    20161 atccgcagcc gtatgtggca ctgccggctt ttgaccgtaa cggcaagtca gctggtatct
    20221 ggctgaaccc gctgaccacg gatgacggaa acgggctgcg gggcttcagt ggtgaaggcc
    20281 gggtgaaagg cagcggggat gcgcagttcg tggccctgca gggcagccgt aacggagaga
    20341 gcctgctggc tgataatatg caggatggtg tccggattgc ccgtgataat cctgacagcg
    20401 gtgtggtggt aagaatcgcc ggtgaaggtc gtccgtggaa tcccggtacc atcaccggtg
    20461 gtcgcgtgtg gggggatatc ccggacaaca gcgttcagcc gggagccgga aatggcgaac
    20521 cggtcacggc agaggtactg gcacagcggc aggctgaaga ggccatccgc cgtgaaacgg
    20581 aacgccgtgc ggatgaaatt gtccgtaaaa tggcagagaa caaacctgac ctgccggacg
    20641 gcaaaacaga gcaggctgtc agggatattg ccgggctgga gcgtgaccgg tctgccatat
    20701 ctgaacggga agccgcgctg ccggagagtg tactgcgtga accacaacgg gtgcgggaag
    20761 cggtccggga ggttgcccgg gaaaatctgc tgcaggagcg actgcagcag atggagcggg
    20821 atatggttcg tgacctgcag aaagagaaaa ccctgggcgg agactgacac aggaagataa
    20881 acgctgatga caacggataa cacgaacaca acacgtaatg attcactggc tgcccggacc
    20941 gatacctggt tgcagtcgtt gctggtctgg tcacccggac agcgggatat catcaaaacg
    21001 gtggcactgg tgctgatggt tctggaccat gcaaaccgca ttctgcatct ggaccagtcg
    21061 tggatgtttc tggtcgggcg tggggccttt ccgctgtttg ccctggtgtg ggggctgaat
    21121 ctgtcccgtc atacgcatat ccggcaggag gccattaacc ggctgtgggg atgggcggtg
    21181 attgcccagt tcgcatatta tctggccggc tttccctggt atgaggggaa tatcctgttt
    21241 gcctttgcag ttgcggcaca ggtgctgaca tggtgtgaaa cgcgcacctg gtggcgttcc
    21301 gcagagacca tgttgcttct ggcaatgtgg ttgcctttct ccggcaccag ttacggcatt
    21361 gccgggctgc tgatgctggc agtcagccac aggctgtacc gggcggaaga cagaatggaa
    21421 cgtctggcgc tggtcgcctg cctgctggct gttattccgg cgcttaacct tgccaccagt
    21481 gatgcagcgg cggtggccgg ccttgtgatg acggtgctga ctgttggtct ggtgtcctgt
    21541 acagggaaat cactaccccg tttctggtac ggggattttt tcccgacgtt ctatgcctgt
    21601 catcttactg tgctgggcgt tctggctgtg tgactggtat gatgctggcg ctgattctgc
    21661 ttattaataa acaacataac acaggatatt tatgaaaatt acggatcaca aactccctga
    21721 aggtattgct ctgacatttc gtgtcccgga aggaaatata aaacatcctt tgattctctt
    21781 atgccatgga ttttgcggta tccggaatgt tctgctgcct tcctttgcga atgcgtttac
    21841 agaagccggg tttgcaacga ttacttttga ttatcgtgga ttcggtgaga gtgaagggga
    21901 acgaggtcgt ctggttccgg caatgcagac cgaagatatt atttcagtta tcaattgggc
    21961 agaaaagcag gtatgtattg ataatcagcg tataggatta tggggaacat cgctgggtgg
    22021 gtgtcatgta ttcaatgcag ctgcgcagga taaaagagtg aaaagtattg tcagccagct
    22081 ggcttttgct gacggtgagg ttctggttac cggagaaatg aatgaactgg agaaagcttc
    22141 ttttctgtcc acgctgaata aaatggcaga aaagaagaaa aataccggga aagagatgtt
    22201 tgtgggggtt acccgggtac tgagtgataa tgaatcaaag gtattcttcg agaaggtaaa
    22261 aggtcagtat cctgaaatgg atataaaaat acctttcctt acggttatgg agacgttgca
    22321 atataaaccg gcagagagcg cagccaaagt acagtgccca gttttggttg ttattgccgg
    22381 ccaggatagt gttaatccac cagaacaggg aagagcctta tatgatgctg ttgcttctgg
    22441 tactaaggaa ttatacgagg aagctgacgc atgccattat gatatttatg aaggcgcgtt
    22501 ttttgaacgt gtggcggcag tacagacgca atggtttaaa aaacatcttt aattgtcggt
    22561 attaatatgt cagaggtcgt tgaggtcagc ttaatatcat agtgagtagt ttcaacgacc
    22621 tgggggacat attttcacaa aggtcagggc aatcatgaca gagcagaaac gaccggtact
    22681 gacactgaaa cggaaaacgg aaggggaaac accgacccgg agccggaaaa ccatcatcaa
    22741 tgtcaccacg ccaccaaaat ggaaggtgaa aaagcagaaa ctggcggaga aggctgcccg
    22801 ggaagcagag ctggcggcga agaaagcaca ggccagacag gcgctgtcca tttatctgac
    22861 gctgccctcg ctggatgagg cggtgaacac tctgaaaccc tggtggccgg gattgtttga
    22921 cggtaacacg ccccggcttc ttgcctgtgg tatccgggag gttttactgg atgaggtgtt
    22981 tcagcggaat atcccgctct ctcataaaaa actcagccgg gcgctgaaag ccatcacccg
    23041 ttcggaaagt tatctgtgtg caatgaaagc cggcgcctgc cgctatgata cggaaggcta
    23101 tgtgacagaa catatcacac aggaagaaga acagtatgcg caggcgcgcc tggaaaaggt
    23161 gcgcagacag aaccggatca aggatgagct gcgggcgatc ctggcagaat aataaaaacg
    23221 ccctctgtat gacagagggc aacagcgaaa aaatcgggaa aacagacatc cagagttttg
    23281 atggagaaaa acacccaacc gttcagggat gtttcataat gtttctgaca taatccggga
    23341 gccgggtcaa gaggggggat aaaaaatggc tgtatccagt ggattatctg agcacagcgg
    23401 tatttatcgc tatcagcgcg tggggcgtca tgccgtccgt caggagattc gtggtttttt
    23461 cagggaagcc gctgaggcct ggcagaatgc ggcccggctg gcaccctgtg aagcatggcg
    23521 ggattttgcc ctgaagagag catctgcatg ccggtgtaag tgccgataaa aataaccccc
    23581 gttgcggagc gccgggggat cagacttata tggcagatcg ttattattat agtggtgtaa
    23641 gaaggagttg ccctgtcttt caaacggaca tcacagaatt aacatatttc acatcccggg
    23701 taagccaaca gggataaaaa atgtgatgaa tttcaagaag cagaaggtga cagtggcatg
    23761 cataacagta cggttatgtc cggtgaatct gacagacagc cgggctgtat tcgcggcagg
    23821 ataaagaaaa accgggagat gactctcccg gtccgtctta tcgctaatcc cctgagcagg
    23881 gcatgtatga cttatctatg accgttatat tcagcgccac atccggtagc ctgcgatata
    23941 acacagttat cctatcagta agagcaggta tgatgaatac acggagatta aaaatataat
    24001 ctccgtcaca tgttaccccc ggaaaccggt aacgccgtca cgggaaatac gtgacagaaa
    24061 aagcagcctc cgttactcca ggtatcagaa ggctgaataa tgcatgagac tttctttctt
    24121 atgtgtctgg tcaggcgggg tgaccagcgt ttttagtaac acatagccat atgtgtatta
    24181 agccacctca tcttattttg tcgaattgat cacaattcaa cggtttattt ttggcgtgat
    24241 gcgtgagccg ttattcatcc cggcatggaa ttcatgcaat cacgaaaagt aatcagtgac
    24301 ggagagtaaa atgaagaaat atataccagt ggtattattt gttttttcat gtcaggtatt
    24361 ttctgctgat attcatggac gtgttgtgcg tgttctggat ggcgatacca ttgacgtcat
    24421 gttatctcag catccggtgc gtgtccgtct tgttaatatt gatgcacctg aaaagaaaca
    24481 ggactatggc cggtggtctg aaaaaataat gaaatctctg gttgcgggaa aaacggttac
    24541 tgtcacatat tttcagcgag atcgttatgg tcgtattctc gggcaggttt atgcaccaga
    24601 cggtatgaat gttaatcagt ttatggtccg tgcaggcgct gcatgggttt atgagcagta
    24661 caacaccgat cctgttcttc ccgtcctgca ggaagaagca cgacagcaga aacgtggtct
    24721 gtgggcagac agcgctcctg ttccgccctg ggtatggcgc tcacggaaat aatgatattt
    24781 ctgtggctat tgagttctca ttgaaatgcg atatttaaaa gcatgaagca tattacatta
    24841 ttaacttatt tctctgttaa tgaaaggtga acaaataaac tatcaggcag tatcaga
//
LOCUS       DAZGAN020000064        24305 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGAN020000064 DAZGAN020000000
VERSION     DAZGAN020000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.064"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            43..342
                     /locus_tag="ACHKYO_RS14580"
                     /old_locus_tag="pgaptmp_002916"
     CDS             43..342
                     /locus_tag="ACHKYO_RS14580"
                     /old_locus_tag="pgaptmp_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429732.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(389..1276)
                     /gene="rarD"
                     /locus_tag="ACHKYO_RS14585"
                     /old_locus_tag="pgaptmp_002917"
     CDS             complement(389..1276)
                     /gene="rarD"
                     /locus_tag="ACHKYO_RS14585"
                     /old_locus_tag="pgaptmp_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HGU4429733.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAISAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1328..1795)
                     /gene="yigI"
                     /locus_tag="ACHKYO_RS14590"
                     /old_locus_tag="pgaptmp_002918"
     CDS             complement(1328..1795)
                     /gene="yigI"
                     /locus_tag="ACHKYO_RS14590"
                     /old_locus_tag="pgaptmp_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HGU4429734.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1960..2829
                     /gene="pldA"
                     /locus_tag="ACHKYO_RS14595"
                     /old_locus_tag="pgaptmp_002919"
     CDS             1960..2829
                     /gene="pldA"
                     /locus_tag="ACHKYO_RS14595"
                     /old_locus_tag="pgaptmp_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HGU4429735.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2962..4791
                     /gene="recQ"
                     /locus_tag="ACHKYO_RS14600"
                     /old_locus_tag="pgaptmp_002920"
     CDS             2962..4791
                     /gene="recQ"
                     /locus_tag="ACHKYO_RS14600"
                     /old_locus_tag="pgaptmp_002920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HGU4429736.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4855..5475
                     /gene="rhtC"
                     /locus_tag="ACHKYO_RS14605"
                     /old_locus_tag="pgaptmp_002921"
     CDS             4855..5475
                     /gene="rhtC"
                     /locus_tag="ACHKYO_RS14605"
                     /old_locus_tag="pgaptmp_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HGU4429737.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5537..6157)
                     /gene="rhtB"
                     /locus_tag="ACHKYO_RS14610"
                     /old_locus_tag="pgaptmp_002922"
     CDS             complement(5537..6157)
                     /gene="rhtB"
                     /locus_tag="ACHKYO_RS14610"
                     /old_locus_tag="pgaptmp_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HGU4429738.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6268..7290
                     /gene="pldB"
                     /locus_tag="ACHKYO_RS14615"
                     /old_locus_tag="pgaptmp_002923"
     CDS             6268..7290
                     /gene="pldB"
                     /locus_tag="ACHKYO_RS14615"
                     /old_locus_tag="pgaptmp_002923"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HGU4429739.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7298..8098
                     /gene="yigL"
                     /locus_tag="ACHKYO_RS14620"
                     /old_locus_tag="pgaptmp_002924"
     CDS             7298..8098
                     /gene="yigL"
                     /locus_tag="ACHKYO_RS14620"
                     /old_locus_tag="pgaptmp_002924"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HGU4429740.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8174..9073
                     /gene="bioP"
                     /locus_tag="ACHKYO_RS14625"
                     /old_locus_tag="pgaptmp_002925"
     CDS             8174..9073
                     /gene="bioP"
                     /locus_tag="ACHKYO_RS14625"
                     /old_locus_tag="pgaptmp_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HGU4429741.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQLHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8961..9914)
                     /gene="metR"
                     /locus_tag="ACHKYO_RS14630"
                     /old_locus_tag="pgaptmp_002926"
     CDS             complement(8961..9914)
                     /gene="metR"
                     /locus_tag="ACHKYO_RS14630"
                     /old_locus_tag="pgaptmp_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HGU4429742.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10032..12293
                     /gene="metE"
                     /locus_tag="ACHKYO_RS14635"
                     /old_locus_tag="pgaptmp_002927"
     CDS             10032..12293
                     /gene="metE"
                     /locus_tag="ACHKYO_RS14635"
                     /old_locus_tag="pgaptmp_002927"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGU4429743.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEVQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12332..13147)
                     /gene="ysgA"
                     /locus_tag="ACHKYO_RS14640"
                     /old_locus_tag="pgaptmp_002928"
     CDS             complement(12332..13147)
                     /gene="ysgA"
                     /locus_tag="ACHKYO_RS14640"
                     /old_locus_tag="pgaptmp_002928"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HGU4429744.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13409..14170
                     /gene="udp"
                     /locus_tag="ACHKYO_RS14645"
                     /old_locus_tag="pgaptmp_002929"
     CDS             13409..14170
                     /gene="udp"
                     /locus_tag="ACHKYO_RS14645"
                     /old_locus_tag="pgaptmp_002929"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HGU4429745.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14311..15738
                     /gene="rmuC"
                     /locus_tag="ACHKYO_RS14650"
                     /old_locus_tag="pgaptmp_002930"
     CDS             14311..15738
                     /gene="rmuC"
                     /locus_tag="ACHKYO_RS14650"
                     /old_locus_tag="pgaptmp_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGU4429746.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15833..16588
                     /gene="ubiE"
                     /locus_tag="ACHKYO_RS14655"
                     /old_locus_tag="pgaptmp_002931"
     CDS             15833..16588
                     /gene="ubiE"
                     /locus_tag="ACHKYO_RS14655"
                     /old_locus_tag="pgaptmp_002931"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HGU4429747.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16602..17207
                     /gene="ubiJ"
                     /locus_tag="ACHKYO_RS14660"
                     /old_locus_tag="pgaptmp_002932"
     CDS             16602..17207
                     /gene="ubiJ"
                     /locus_tag="ACHKYO_RS14660"
                     /old_locus_tag="pgaptmp_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HGU4429748.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17204..18844
                     /gene="ubiB"
                     /locus_tag="ACHKYO_RS14665"
                     /old_locus_tag="pgaptmp_002933"
     CDS             17204..18844
                     /gene="ubiB"
                     /locus_tag="ACHKYO_RS14665"
                     /old_locus_tag="pgaptmp_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HGU4429749.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18923..19192
                     /gene="tatA"
                     /locus_tag="ACHKYO_RS14670"
                     /old_locus_tag="pgaptmp_002934"
     CDS             18923..19192
                     /gene="tatA"
                     /locus_tag="ACHKYO_RS14670"
                     /old_locus_tag="pgaptmp_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HGU4429750.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19196..19711
                     /gene="tatB"
                     /locus_tag="ACHKYO_RS14675"
                     /old_locus_tag="pgaptmp_002935"
     CDS             19196..19711
                     /gene="tatB"
                     /locus_tag="ACHKYO_RS14675"
                     /old_locus_tag="pgaptmp_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HGU4429751.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19714..20490
                     /gene="tatC"
                     /locus_tag="ACHKYO_RS14680"
                     /old_locus_tag="pgaptmp_002936"
     CDS             19714..20490
                     /gene="tatC"
                     /locus_tag="ACHKYO_RS14680"
                     /old_locus_tag="pgaptmp_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HGU4429752.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20532..21314
                     /gene="tatD"
                     /locus_tag="ACHKYO_RS14685"
                     /old_locus_tag="pgaptmp_002937"
     CDS             20532..21314
                     /gene="tatD"
                     /locus_tag="ACHKYO_RS14685"
                     /old_locus_tag="pgaptmp_002937"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HGU4429753.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQCIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21311..21799)
                     /gene="rfaH"
                     /locus_tag="ACHKYO_RS14690"
                     /old_locus_tag="pgaptmp_002938"
     CDS             complement(21311..21799)
                     /gene="rfaH"
                     /locus_tag="ACHKYO_RS14690"
                     /old_locus_tag="pgaptmp_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HGU4429754.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21966..23459
                     /gene="ubiD"
                     /locus_tag="ACHKYO_RS14695"
                     /old_locus_tag="pgaptmp_002939"
     CDS             21966..23459
                     /gene="ubiD"
                     /locus_tag="ACHKYO_RS14695"
                     /old_locus_tag="pgaptmp_002939"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HGU4429755.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23505..24206
                     /gene="fre"
                     /locus_tag="ACHKYO_RS14700"
                     /old_locus_tag="pgaptmp_002940"
     CDS             23505..24206
                     /gene="fre"
                     /locus_tag="ACHKYO_RS14700"
                     /old_locus_tag="pgaptmp_002940"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HGU4429756.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
BASE COUNT         5719 a         6033 c         6661 g         5892 t
ORIGIN      
        1 atttttattc tataaacaat atcttgggtg aatggattct atatgggaaa tggtaacttt
       61 tggcagttgt tcttgggtaa agaaccttac agcaaaaaat ctgattttgt taaggcatgg
      121 ttattgtggt gggctattac catccttgtt ctatttattt taagcctgac tacgacatta
      181 aatttaagaa gtataggtat agggttaagt tttgggtttt gtcaaatcct tggtagatat
      241 acgatgtctc taggatggag taagtactgg ggagttttgg gggtatttcc tgtaacaaat
      301 attgttcttt ttcttatctt aatttttttc aaaaaaagat aaataagtca gtgggtaggc
      361 tctttaaacg gcctacccat gttaattctt aactttttct acgctgcgta taaatcgcat
      421 ccatcacaaa aattgccagc gccacccaaa taaaggcgaa agtcaccatc ttatcggcac
      481 ccggtttttc accataaaac gtcacagcca gcaggaacat cagcgtcggg ccaatgtact
      541 ggaaaaagcc taacgttgag agacgcaagc gcgtggcagc ggcggtaaaa cacaacagcg
      601 gtacggtagt gacaataccg gcggcgatca gcagtaaatt cagcgacatc gggttttgcc
      661 ccatatggct ggttgagctg tcggcaatag caaacaggta aattgccgcc acgggcagca
      721 gccacatggt ttcgattaac atgccggttt gcgcttcaac ggcaatcttc ttgcgtacca
      781 gaccgtagaa ggcaaaacta aatgccagtc ccagcgcgat aataggtagc gaaccaaaag
      841 tccacagctg gactaacacg ccacatatcg ccagaatcac cgccagccat tgcatccggc
      901 ggaatcgctc gccgaggaaa atcatcccca gcacaatgtt caccagcggg ttaataaagt
      961 aaccaaggct cgcttccagc atatggtgat tgttcaccgc ccagataaac agtagccagt
     1021 tgccaccaat cagcacggca gagattgcca gcataaaaat tttctgtggc gtctgaatca
     1081 gcgtttttaa ataggaccac tggcggcaaa tgctcatcag caccaccata aagaaaaacg
     1141 accagatcac gcgatgcgtc aggatttcat cggcaggcac gtagtaaatc aacttgaagt
     1201 acgctggcgc tataccccaa ataaaataag cggcaagagc gagtaatacg ccctgccgcg
     1261 tttgttttgc atccatcggg aatactcatt tttaattggt aacagcagtt tacctgcttt
     1321 tatgtcttca acctaccata taggtggcgg tggcactggc aatataaagc tgttcttcat
     1381 tgtgtaattc aacgcgggcg acggcgactt tattgcctgc acgcaacagg ctactggtag
     1441 cagtaaaacg ctcgcccctg cctgggcgca gataatcaac gcgaagatca atggtcccca
     1501 tccgcgatag ccgctggcgt agttcatctt cactgatggt ttcgtggcgg gttaaggtac
     1561 ttcccacgca caccagaccg gcggcgacat ccagcgccga cgcaatgacc ccgccgtgca
     1621 aaatgctttg cgcccagttg cccaccatca ttggctgatt tttaaaggcc agctgtgcga
     1681 actctttttc gtaacgctcc agttccatcc ccaatgcgcg gttaaatggc atgtgataaa
     1741 caaacatctc acccactaat ttcagggctt gttcagcggt cagtacggca gacatatcat
     1801 ccttacactt cattggttaa tgaaatgttg attttatgct tctttgttgt tggtttctac
     1861 tttaggaagg gataactaac ggctatggag ttaagtatgt aaaatagccc gcagaaaaat
     1921 attcacctta tcaataattc gttacggaga acacgaccga tgcggactct gcagggctgg
     1981 ttgttgccgg tgtttatgtt gcctatggca gtatatgcgc aagaggcaac ggtgaaagag
     2041 gtgcatgacg cgccagcggt gcgtggcagt attatcgcca atatgctgca ggagcatgac
     2101 aatccgttca cgctctatcc ttatgacacc aactacctca tttacaccca aaccagcgat
     2161 ctgaataaag aagcgattgc cagttacgac tgggcggaaa atgcgcgtaa ggatgaagta
     2221 aagtttcagt tgagcctggc atttccgctg tggcgtggga ttttaggccc gaactcggtg
     2281 ttgggtgcgt cttatacgca aaaatcctgg tggcaactgt ccaatagcga agagtcttca
     2341 ccgtttcgtg aaaccaacta cgaaccgcaa ttgttcctcg gttttgccac cgattaccgt
     2401 tttgcaggtt ggacgctgcg cgatgtggag atggggtata accacgactc taacgggcgt
     2461 tccgacccga cctcccgcag ctggaaccgc ctttatactc gcctgatggc agaaaacggt
     2521 aactggctgg tagaagtgaa gccgtggtat gtggtgggta atactgacga taacccggat
     2581 atcaccaaat atatgggtta ctaccagctt aaaatcggct atcacctcgg tgatgcggtg
     2641 ctcagtgcga aaggacagta caactggaac accggctacg gcggcgcgga gttaggctta
     2701 agttacccga tcaccaaaca tgtgcgcctt tatactcagg tttacagcgg ctatggcgaa
     2761 tcgctcatcg actataactt caaccagacc cgtgtcggtg tgggggttat gctaaacgat
     2821 ttgttttgat gaacggttga gtggttggca aatctggaat ccagcatcca ggattaccct
     2881 ctcagagact aaaagcattg cagtttctcg cgcaggcgct gaaaatagcg cctgttttta
     2941 tttcaggcaa tcggggtgaa tgtggcgcag gcggaagtgt tgaatctgga gtccggagct
     3001 aaacaggttt tacaagaaac ctttggctac caacagtttc gccccggcca ggaagaaatt
     3061 atcgacactg tgctttccgg tcgcgattgc ctggtcgtca tgcccaccgg tggcggaaaa
     3121 tccctttgct atcaaattcc tgccttattg ctaaacggcc ttaccgtggt tgtttcaccg
     3181 ctgatttcgt tgatgaaaga tcaggtggat caactgcaag ccaacggcgt ggcggcggcg
     3241 tgccttaact cgacgcaaac ccgcgaacag caacttgaag tgatgacagg ctgccgcacc
     3301 gggcaaattc gtctgcttta tatcgccccg gaacgcctga tgctggataa ctttcttgag
     3361 catctggcgc actggaatcc ggtgttatta gccgttgatg aagcgcactg tatctcccaa
     3421 tggggccacg atttccgccc ggaatatgcc gcgctcggtc agttgcgcca gcggttcccg
     3481 acgctgccgt ttatggcgct gaccgccaca gccgacgaca ccacgcgcca ggatatcgtg
     3541 cgcctgctgg ggctgaacga tccgctgatt caaatcagca gttttgaccg tccgaatatt
     3601 cgctacatgc tgatggagaa gttcaaaccg ctcgatcagt tgatgcgcta cgtgcaggaa
     3661 cagcgcggta agtccggcat tatctactgc aacagccggg cgaaagtaga agacaccgct
     3721 gcgcgcctgc aaagcaaggg tattagcgcg gcggcctatc atgccgggct ggaaaataat
     3781 gtccgcgccg acgtgcagga gaaattccag cgcgatgacc tgcaaattgt ggtggcgacg
     3841 gtggcgttcg gcatgggcat caataaacca aacgttcgct tcgtggtcca ctttgatatt
     3901 ccgcgcaata tcgaatccta ttatcaggaa accggtcgcg ccgggcgtga tggcctgccc
     3961 gcggaagcga tgctgtttta cgatccggcc gatatggcgt ggctgcgccg ttgtctggaa
     4021 gagaagccgc aggggcagtt gcaggatatc gaacgtcata aactcaatgc gatgggcgct
     4081 tttgccgaag cgcaaacttg ccgtcgtctg gtactgctga actattttgg cgaagggcgt
     4141 caggagccgt gcgggaactg cgatatctgc ctcgatccgc cgaaacagta cgacggttca
     4201 accgatgctc agattgccct ttccaccatt ggtcgtgtga atcagcgttt tggtatgggc
     4261 tatgtggtgg aagtgattcg cggcgctaat aaccagcgta tccgcgacta tggtcatgac
     4321 aaactaaaag tctacggtat gggccgtgat aaaagtcatg aacattgggt gagcgtgatc
     4381 cgccagctga ttcacctcgg tctggtgacg caaaatattg cccagcattc tgccctacaa
     4441 ctgacagagg ctgcgcgtcc ggtgctgcgc ggcgaatcct ctttgcaact tgccgtgccg
     4501 cgtatcgtgg cgctcaaacc gaaagcgatg cagaaatcgt tcggcggcaa ctatgatcgc
     4561 aaactgttcg ccaaattacg caaactgcgt aaatcgatag ccgatgaaag taatgtcccg
     4621 ccgtacgtgg tgtttaacga cgcaaccttg attgagatgg ctgaacagat gccgatcacc
     4681 gccagcgaaa tgctcagcgt taacggcgtt gggatgcgca agctggaacg ctttggcaaa
     4741 ccgtttatgg cgctgattcg tgcgcatgtt gatggcgatg acgaagagta gtcagcagca
     4801 taaaaaagtg ccagtatgaa gactccgtaa acgtttcccc cgcgagtcaa atgtatgttg
     4861 atgttatttc tcaccgtcgc catggtgcac attgtggcgc ttatgagccc cggtcccgat
     4921 ttcttttttg tctctcagac cgctgtcagt cgttcccgta aagaagcgat gatgggcgtg
     4981 ctgggcatta cctgcggcgt aatggtttgg gctgggattg cgctgcttgg cctgcatttg
     5041 attatcgaaa aaatggcctg gctgcatacg ctgattatgg tgggcggtgg cctgtatctc
     5101 tgctggatgg gttaccagat gctacgcggt gcactgaaaa aagaggcggt ttctgcacct
     5161 gcgccacagg tcgagctggc gaaaagtggg cgcagtttcc tgaaaggttt actgaccaat
     5221 ctcgctaatc cgaaagcgat tatctacttt ggctcggtgt tctcattgtt tgtcggtgat
     5281 aacgttggca ctaccgcgcg ctggggcatt tttgcgctga tcattgtcga aacgctggcg
     5341 tggtttaccg tcgttgccag cctgtttgcc ctgccgcaaa tgcgccgtgg ttatcaacgt
     5401 ctggcgaagt ggattgatgg ttttgccggg gcgttatttg ccggatttgg cattcatttg
     5461 attatttcgc ggtgatgcca gacgcgtctt cagagtaagt cggataaggc gtttacgccg
     5521 catccgacat tatttttcac gcatgcctcg ccgatgctaa cagcgctccc accagcataa
     5581 acaacgagcc gaaaatctta ttcagcgcct tcatctgctt tggtccttta atccatagag
     5641 caatccgttg agcaagggtg gcgtaaccga tcatcacaat aatatcgacc acaatagtgg
     5701 tgacgccgag cacgatatac tgcatcagtt gcggctgttg cggcatgatg aattgcggaa
     5761 atagcgccgc cagaaacaca atacttttgg gattggtgag attcacaaaa actgcgcgct
     5821 ggaacaaatg tcgacgcgat tgagtagagg ccagcgattt aaggtcaatt gcaccagcgg
     5881 cgcgccactg ctggattccc agccaaatca agtaagccgc gcctgcccac ttcaacactt
     5941 caaacgcaat cactgagcgg gaaaatagcg tccccaaccc cacgccaacc aacacaatat
     6001 gaatcgccag tccggtctga agcccagcaa tagacgccac cgcgccgcga taaccgtggt
     6061 tgagcgaggt ggtcatagtg ttgattgcac cagagcctgg cgacaggctt aaaatgatcg
     6121 atgtcagcag gtaggcaaac caccattcta aggtcatgat gaactcccgg tgtgtctatt
     6181 tttgtgccac aatacgctac tgtcgcagcg ttgtgtcagg cacgctaaaa aaaacgattt
     6241 tacgtggttt aagaggcaga ttacccgatg tttcagcagc aaaaagactg ggaaacaaga
     6301 gaaaacgcgt ttgctgcttt taccatggga ccgctgactg atttctggcg tcagcgtgat
     6361 gaagcagagt ttactggtgt ggatgacatt ccggtgcgct ttgtccgttt tcgcgcacag
     6421 caccatgacc gggtggtagt catctgcccg gggcgtattg agagctacgt aaaatatgcg
     6481 gaactggcct atgacctgtt ccatttgggg tttgatgtct taatcatcga ccatcgcggg
     6541 cagggacgtt ccggtcgcct gttagccgat ccgcatctcg ggcatgttaa tcgctttaat
     6601 gattatgttg atgatctggc ggcattctgg cagcaggagg ttcagcccgg tccgtggcgt
     6661 aaacgctata tactggcaca ttcgatgggc ggtgcgatct ccacattatt tctgcaacgc
     6721 catccaggtg tatgtgacgc cattgcgcta actgcgccaa tgtttgggat cgtgattcgt
     6781 atgccgtcat ttatggcacg gcagatcctc aactgggccg aagcgcatcc acgtttccgt
     6841 gatggctatg caataggcac cgggcgctgg cgcgcgttgc cgtttgctat caacgtactg
     6901 acccacagca gacagcgata tcgacgtaac ttacgcttct atgctgatga cccaacgatt
     6961 cgcgtcggtg ggccgaccta ccattgggta cgcgaaagta ttctggctgg cgaacaggtg
     7021 ttagccggtg cgggtgatga cgccacgcca acgcttctct tacaagctga agaggaacgc
     7081 gtggtggata accgcatgca tgaccgtttt tgtgaactcc gcaccgccgc gggccatcct
     7141 gtcgaaggag gacggccgtt ggtaattaaa ggtgcttacc atgagatcct ttttgaaaag
     7201 gacgcaatgc gctcagtcgc gctccacgcc atcgttgatt ttttcaacag gcataactca
     7261 cccagcggaa accgctctac agaggtttaa atttcttatg taccaggttg ttgcgtctga
     7321 tttagatggc acgttacttt ctcccgacca tacgttatcc ccttacgcca aagaaactct
     7381 gaagctgctc accgcgcgcg gcatcaactt tgtgtttgcg accggtcgtc accacgttga
     7441 tgtggggcaa attcgcgata atctggagat taagtcttac atgattacct ccaatggtgc
     7501 gcgcgttcac gatctggatg gtaatctgat ttttgctcat aacctggatc gcgacattgc
     7561 cagcgatctg tttggcgtag tcaacgacaa tccggacatc attactaacg tttatcgcga
     7621 cgacgaatgg tttatgaatc gccatcgccc ggaagagatg cgctttttta aagaagcggt
     7681 gttcaaatat gcgctgtatg agcctggatt actggagccg gaaggcgtca gcaaagtgtt
     7741 tttcacctgc gattcccatg aacaactgct gccgctggag caggcgatta acgctcgctg
     7801 gggcgatcgc gtcaacgtca gtttctctac cttaacctgt ctggaagtga tggcgggcgg
     7861 cgtgtcgaaa ggccatgcgc tggaagcagt ggcgaagaaa ctgggctaca gcctgaagga
     7921 ttgtattgcg tttggtgacg ggatgaacga cgccgaaatg ctgtcgatgg cggggaaagg
     7981 ctgcattatg ggcagtgcgc accagcgtct gaaagacctt catcccgagc tggaagtgat
     8041 tggtactaat gccgacgacg cggtgccgca ttatctgcgt aaactctatt tatcgtaatc
     8101 gttctttatt tggtcagttg tcaacctgat acttcgctac aatggatact cgttaatcaa
     8161 agagttttcc attgtggcgc tacttatcat caccacgatt ctgtgggcct tctcctttag
     8221 cttttatggc gagtaccttg cggggcacgt cgatagctat tttgcggtgc tggtgcgcgt
     8281 tggcctggcg gcactcgttt ttctgccgtt tctgcgtacc cgtggcaata gcctgaaaac
     8341 ggtcggcctg tatatgttgg tgggcgcgat gcagctcggc gtgatgtata tgctgagttt
     8401 ccgcgcgtat ctctacctga cggtctccga actgctgctg ttcaccgtac tgacgccgct
     8461 ctacatcacg ctgatttatg acatcatgag taagcgccgt ctgcgctggg gctatgcttt
     8521 tagcgccttg ctggcggtga ttggtgccgg gattattcgc tatgatcagg tcaccgacca
     8581 tttctggact ggcttgttgc tggtgcaact ctccaatatc acttttgcca tcggcatggt
     8641 gggttacaaa cgcctgatgg aaactcgccc gatgccgcag cataacgcct ttgcgtggtt
     8701 ctatcttggc gcgttcctgg tggcggtaat tgcatggttc ttgctgggaa atgcgcagaa
     8761 aatgccgcaa accacgttgc aatggggcat tctggtgttt cttggcgtgg tggcttccgg
     8821 gattggctac tttatgtgga actacggcgc gacgcaggtg gacgccggaa cgctgggcat
     8881 tatgaataat atgcacgttc cggcagggct gctggttaac ctggctatct ggcaccaaca
     8941 gctgcactgg ccaacgttta ttacaggcgc gctggtgatc ctggcctcac tgtgggtgca
     9001 tcgtaagtgg gtcgctccgc gctcttcaca aacggcagat gatcgcaggc gtgattgcgc
     9061 gctgagcgaa taaacgcttc cgtaactggc tgacgctgct cgccatcgcg cacggcggcg
     9121 tacagtcggc tccacaagcc ttcgcccagg gttttggtca ccaccagacc ctggcgctca
     9181 aaactctcta ctacccaatg cggtagcgcg gcaataccca tccgcgcggc aaccatctga
     9241 atcaacaata aggtgttatc gacgcttttc agtgacgggc tgacgcctgc cggctgaaga
     9301 aaatgccgcc agacatccag tcgactacgc tgcaccggat aaattaatag cgtctcgctg
     9361 gcgagatctt ccggtgtaat acgcgttttt gccgccagtg gatggtcagg tgctaacacc
     9421 aggcgtactt cataatcgaa catcggcgaa taatgcaggc cactgcgcgg caggatatcg
     9481 gaggtcatca ccagatccag ctcgccctgt tgcaacgcgg gctgcgggtc aaacgtcacg
     9541 cccgatttaa aatccatctc tacctgcggc cagttcttat ggaaattttc taacgcgggt
     9601 gtcagccact gaatacagct atggcactca atggcaatgc gcagacgcgt ctgctgcggt
     9661 tcattgcagg cttgtagtgc ctggctgatt tgcggcagca cctggtttgc cagttgcaac
     9721 aggatttctc cctgcggtgt aaagcgtagc ggctggctct tacgcacaaa tagccggaaa
     9781 ccaaggcgtt gttccagatc gctaaactgg tgagacaggg cggattgcgt ctgatgcaac
     9841 gtcgccgcag cggctgcgag cgagccgcag ttccgcaacg cttgtagcgt tttcaggtgt
     9901 tttacttcga tcatgaaagt ccttcacttc ggcatgaata atttgcgctt gaggaatata
     9961 cagtaaccgc caattatgga tgtgtaaaca tctggacggc taaatactaa aaattccata
    10021 aggggcatat aatgacaatt cttaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc
    10081 gcgagctgaa aaaagcgcaa gaaagttatt gggcggggaa ctccacgcgt gaagaactgc
    10141 tgacagtagg gcgtgaactg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg
    10201 acctgctgcc ggtgggcgat tttgcctggt acgatcatgt actgaccacc agtttgctgc
    10261 tgggtaatgt tccgccacgt catcagaaca aagacggttc ggtagatatc gacaccctgt
    10321 tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca
    10381 aatggtttaa caccaactat cactacatgg tgccggagtt cgttaaaggc caacagttca
    10441 aactgacctg gacgcagctg ctggaggaag tggacgaggc gctggcgctg ggccacaacg
    10501 ttaagcctgt gctgctgggg ccggttactt acctgtggct ggggaaagtg aaaggtgaac
    10561 agtttgaccg ccttagcctg ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag
    10621 aactggcgaa acgcggcatc gagtgggtac agattgatga acccgcgctg gtactggaac
    10681 taccacaggc gtggctggac gcatacaaac ccgcttacga cgcgctccag ggacaggtga
    10741 aactgctgct gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc
    10801 tgcctgttca gggtctgcat gttgacctcg tacatggtaa agatgacgtt gctgaactgc
    10861 acaagcgcct gccttctgac tggttgctgt ctgcgggtct gatcaatggt cgtaacgtct
    10921 ggcgcgccga tcttaccgag aaatatgcgc aaattaagga cattgtcggc aaacgtgatt
    10981 tgtgggtggc atcttcctgc tcgttgctgc acagccccat cgacctgagc gtggaaacgc
    11041 gtcttgatgc agaagtgaaa agctggtttg ccttcgccct acaaaaatgc catgaactgg
    11101 cactgctgcg cgatgcgctg aacagtggtg acacggcagc tctggcagag tggagcgccc
    11161 caattcaggc gcgtcgtcac tctactcgtg tacataatcc ggcggtagaa aagcgtctgg
    11221 cggcgatcac cgcccaggac agccagcgtg cgaatgtcta tgaagtgcgt gctgaagtcc
    11281 agcgtgcgcg ttttaaactg ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa
    11341 ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg caatctcgac gccaacaact
    11401 accgcacggg cattgcggaa catatcaagc aggccattgt tgagcaggaa cgtttgggac
    11461 tggatgtgct ggtacatggc gaggccgagc gtaatgacat ggtggaatac tttggcgagc
    11521 acctcgacgg atttgtcttt acgcaaaacg gttgggtaca gagctacggt tcccgctgcg
    11581 tgaagccacc gattgttatt ggtgacgtga gccgaccggc accgattacc gtggagtggg
    11641 cgaaatatgc gcagtcgctg accgacaaac cggtgaaagg gatgctgact ggtccggtga
    11701 cgattctctg ctggtcgttc ccgcgtgaag atgtgagccg tgaaaccatc gccaaacaga
    11761 ttgcgctggc gctgcgtgat gaagtggcgg acctggaagc cgctggaatt ggcatcatcc
    11821 agattgatga accggcgctg cgcgaaggtt taccgctgcg tcgtagcgac tgggacgcct
    11881 atctccagtg gggcgtagag gccttccgta tcaacgccgc cgtggcgaaa gatgacacac
    11941 aaatccacac tcacatgtgt tattgcgagt tcaacgacat catggattcc attgcggcgc
    12001 tggatgcgga tgtgattacc atcgaaactt cgcgttctga catggagttg ctggagtcgt
    12061 tcgaagagtt tgattatccc aatgaaatcg gtccaggcgt ctatgatatt cactcgccaa
    12121 acgtaccgag cgtggaatgg attgaagccc tgctgaagaa agcggcaaaa cgcattccgg
    12181 cagagcgcct gtgggttaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc
    12241 gcgcggcact ggcgaacatg gtgcaggcgg cgcagaactt gcgtcggggg taaatccaaa
    12301 ccgggtggta ataccacccg gtcttttctc attacagcga cttcttccca ccatactgct
    12361 taaaccattc cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc
    12421 gataatcagc gttgaatgca tgcccggcat ccgggtacac gataatctct gctttcgcat
    12481 tagcagcccg cagcgcctgg cgcatggttt caacgctctc ctgcggaatg ctgttatcct
    12541 gaccaccata taagccgaga accggcgcgt taagatcggt tgcgatatca acaggttgtt
    12601 tcggtgaatt cagcgacttg tcgcccgtca gtttgccgta ccacgccact gcggctttta
    12661 gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag aatccggtga
    12721 tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat
    12781 cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagcaac gtggggatat
    12841 cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc
    12901 cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct
    12961 gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca tggtaagcag
    13021 gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccgggg
    13081 tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat tgttgtgttg
    13141 ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca
    13201 tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca gaatgaagag
    13261 tgatttatgt gatttacatc acttttgatg ggtaaattta tgcaacgcgt ttgcgtcatg
    13321 gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg
    13381 actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct
    13441 cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt
    13501 ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac
    13561 tacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgttctaccg gtatcggcgg
    13621 cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg
    13681 tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac
    13741 ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt
    13801 cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg aaatccattg gcgcgacaac
    13861 tcacgttggc gtgacagctt cttctgatac cttctaccca ggtcaggaac gttacgatac
    13921 ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg
    13981 cgtaatgaac tatgaaatgg aatctgcaac cctgctgacc atgtgtgcaa gtcagggcct
    14041 gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc cagcaagaga tcccgaatgc
    14101 tgagacgatg aaacaaaccg aaagccatgc ggtgaaaatc gtggtggaag cggcgcgtcg
    14161 tctgctgtaa ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc
    14221 gtagcgcctc gcaggaaatg cctttccaac tggacgtttg tacagcacaa ttctattttg
    14281 tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct caatcatggt ttacgcagtt
    14341 attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa
    14401 aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa
    14461 caaattaccc aaagcgagca ctggcgtgca gagtgcgagt tactcaataa cgaagtgcgc
    14521 agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg
    14581 gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc
    14641 ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg agcacagcaa tcgccgggtt
    14701 gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac
    14761 ggtttccgcc gtcaggttca ggacagcttc ggtaaagaag cacaagaacg ccataccctg
    14821 acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga agcgatcaac
    14881 ctgacgcgcg cgctgaaagg cgacaataaa acccagggca actggggcga ggtagtattg
    14941 acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc
    15001 atcgaaaatg acgcccgctc gcggatgcaa ccggatgtca tcgtgcggct gccgcaggga
    15061 aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta tttcaacgcc
    15121 gaagatgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat
    15181 atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac
    15241 gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag
    15301 ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg
    15361 gcgttgcgta ctattgccaa cctgtggcgt tatgagcatc aaagccgcaa cgcccagcaa
    15421 atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc
    15481 gcgattggtc aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaactc
    15541 tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa
    15601 attaaacgcg agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat
    15661 cgacttcggt cggttccgga gcagccgaat gatgaagctt atcaacgcga tgatgaatat
    15721 aatcagcagt cgcgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg
    15781 acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga
    15841 taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga
    15901 tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat
    15961 gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg
    16021 tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg
    16081 cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat
    16141 gggacgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa
    16201 cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct
    16261 gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg
    16321 cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta
    16381 tgatgcatac tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga
    16441 cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc
    16501 catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt
    16561 ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag
    16621 tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcacct gcgctgaaaa
    16681 cagcccgctc gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt
    16741 cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg
    16801 ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc
    16861 ttaccgcact gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa
    16921 acttcgttgc gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata
    16981 ccggtgatat cgccgctgaa ggaatcagca aagccatgcg cggaggcgca aagttcctgc
    17041 atcacggcat taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta
    17101 tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg
    17161 ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc caggtgaagt
    17221 acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc
    17281 caaaatgcgt atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa
    17341 tcggcataaa gacaaacttt taggtgagcg actacgactg gccctgcaag aactggggcc
    17401 ggtttggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc caccgcatat
    17461 tgccgatcag ctggcgttat tgcaggacaa agttgctccg tttgatggca agctggcgaa
    17521 gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga
    17581 aatcaagccg ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa
    17641 tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtca ttaaagcgga
    17701 tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg
    17761 tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt
    17821 gctgcgggaa tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct
    17881 ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat
    17941 ttacggcatt ccggtgtctg atgttgcggc gctggagaaa aacggcacta acatgaaatt
    18001 gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca
    18061 tgccgatatg caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata
    18121 tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc
    18181 agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga
    18241 ttctggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt
    18301 ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa
    18361 tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca
    18421 gaaaaccctg ctctatgtcg aaggggtagg acgccagctt tatccgcagc tcgatttatg
    18481 gaaaacagcg aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgcgct
    18541 ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga
    18601 attggtttac gacagtttgc gccagggcaa gtatttacag cacagtgttg ataagattgc
    18661 ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg
    18721 cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgagt gggggctgat
    18781 gcccggctgg ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg
    18841 ctgatttttt catcgctcaa ggcgggccgt gtaacgtata atgcggcttt gtttaatcat
    18901 catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc
    18961 cgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg
    19021 tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac
    19081 cagtcaggat gctgatttta ctgcgaaaac tatcgccgat aagcaggcgg atacgaatca
    19141 ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt
    19201 tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc
    19261 gcaacgactg cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact
    19321 ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag
    19381 tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat
    19441 ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga
    19501 aaaggcgagc gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc
    19561 gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc
    19621 agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gctgaaccga aaaccgctgc
    19681 accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt
    19741 atcacgcatc tgattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg
    19801 atattcctgt gtctggtcta tttcgccaat gatatctatc acctggtatc cgcgccattg
    19861 atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt
    19921 acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat
    19981 caggtgtggg catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg
    20041 ctgctggttt ccagctctct gctgttttat atcggtatgg cattcgccta ctttgtggtc
    20101 tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtgca ggtatccacc
    20161 gacatcgcca gctatttaag cttcgttatg gcgctgttta tggcgtttgg tgtctccttt
    20221 gaagtgccgg tggcaattgt gctgctgtgc tggatgggga ttacctcgcc agaagactta
    20281 cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgtt gctgacgccg
    20341 ccggatgtct tctcgcaaac gctattggcg atcccgatgt actgtctgtt tgaaatcggt
    20401 gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct
    20461 gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata
    20521 tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc
    20581 gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca
    20641 ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt
    20701 caacggcggg cgtacatcct cacgacagca gccagtggca agctgcgact gaagaagcga
    20761 ttattgagct ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta
    20821 accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag ctacgcattg
    20881 ccgcagaatt aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga
    20941 cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca
    21001 cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg
    21061 tttgcgatga acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga
    21121 aattgctgat cgaaactgat gcgccgtatc tgctcccgcg cgatctcacg ccaaagccat
    21181 catcccggcg caacgagcca gcccatctgc cccatatttt gcaatgtatt gcgcactggc
    21241 gtggagaaga tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg
    21301 ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat
    21361 taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct
    21421 gaaagccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa ggggttgccg
    21481 gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta
    21541 tcgctggcga cgcgccaaag cgcacgaagt ggctgacacc gcgggtcgcg ttgatagtcg
    21601 tggtatgaat cacttctgga tcaaattcga caaacaggta gttgggaaac aatggctcac
    21661 tgactgcagt acgttttcca cgcacgattt tttccagggt gatcatcggt gccaggcaat
    21721 tcacagcctg tctttcgagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca
    21781 gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa
    21841 agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta
    21901 atttaacaaa tttacagcat cgcaaagatg aacgccgtat aatgggcgta gattaagagg
    21961 ctacaatgga cgccatgaaa tataacgatt tacgcgactt cctgacactg cttgaacagc
    22021 agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg
    22081 ctgaccgcac tttgcgtgcc ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact
    22141 caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcaatg ggcatggggc
    22201 aggaagatgt ttcggcgcta cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg
    22261 agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag caagtattga
    22321 acatgccgac aaagcggctg cgtggtgcgc cctgccaaca aaaaatcgtc tctggcgatg
    22381 acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc gcgccgctga
    22441 ttacctgggg gctgacagtg acgcgcggcc cgcataaaga gcggcagaat ctgggcattt
    22501 atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg
    22561 gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg
    22621 tggcgctggg tgccgatccc gccacgattc tcggtgcagt cacccccgtt ccggatacac
    22681 tttcagagta tgcgtttgcc ggattgctgc gcggtaccaa gaccgaagtg gtgaagtgta
    22741 tctccaatga ccttgaagtg cccgccagtg cggagattgt gctggaaggg tatatcgaac
    22801 aaggtgaaac ggcgccggaa gggccgtatg gcgaccacac cggttactat aacgaagtcg
    22861 atagtttccc ggtatttacc gtgacgcata ttacccagcg tgaagatgcg atttaccact
    22921 ccacctatac tggtcgtccg ccggatgaac ccgcggtgct gggtgtcgca ctgaacgaag
    22981 tgtttgtgcc gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg
    23041 aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga
    23101 agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga
    23161 tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca
    23221 cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg
    23281 attttgcctc gcctgtctcc gggctgggtt caaaaatggg gctggatgcc acgaataaat
    23341 ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtcg
    23401 cgcatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat
    23461 gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct
    23521 gtaaagtgac ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag
    23581 atgcggcctt ttcttttcgc gctggtcagt atttgatggt agtgatggat gagcgcgaca
    23641 aacgtccgtt ctcaatggct tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg
    23701 gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc
    23761 aaatcgtggt cgatattccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga
    23821 tgattttgat tgcgggcggc accgggttct cttatgcccg ctcgattttg ctgacagcgt
    23881 tggcgcgtaa cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc
    23941 tgtatgatct ctgcgagctt gaggcgcttt cgttgaagca tcctggtctg caagtggtgc
    24001 cggtggttga acaaccggaa gcgggctggc gtgggcgtac tggcaccgtg ttaacggcgg
    24061 tattgcagga tcacggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga
    24121 tggcgaaaat tgcccgcgat ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt
    24181 ttggcgatgc gtttgcattt atctgagata taaaaaaacc cgcccctgac aggcgggaag
    24241 aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg gcattcggca
    24301 cacga
//
LOCUS       DAZGAN020000065        24250 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGAN020000065 DAZGAN020000000
VERSION     DAZGAN020000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.065"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..248
                     /locus_tag="ACHKYO_RS14705"
                     /old_locus_tag="pgaptmp_002941"
     CDS             <1..248
                     /locus_tag="ACHKYO_RS14705"
                     /old_locus_tag="pgaptmp_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429757.1"
                     /translation="IFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFR
                     YIRFLNKRARKRLNTKLFKVQPYPKPQTVKTGLKESE"
     gene            342..1472
                     /locus_tag="ACHKYO_RS14710"
                     /old_locus_tag="pgaptmp_002942"
     CDS             342..1472
                     /locus_tag="ACHKYO_RS14710"
                     /old_locus_tag="pgaptmp_002942"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU4429758.1"
                     /translation="MQIGIKKIAALYSCSTFAAPQQINDIVHRTITPLIEQQKIPGMA
                     VAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLSD
                     PTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPG
                     TQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGY
                     REGKAVHVSPGALDAKTYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYWQ
                     TGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGAT
                     GGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1561..2094
                     /gene="blc"
                     /locus_tag="ACHKYO_RS14715"
                     /old_locus_tag="pgaptmp_002943"
     CDS             1561..2094
                     /gene="blc"
                     /locus_tag="ACHKYO_RS14715"
                     /old_locus_tag="pgaptmp_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU4429759.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2091..2408)
                     /gene="sugE"
                     /locus_tag="ACHKYO_RS14720"
                     /old_locus_tag="pgaptmp_002944"
     CDS             complement(2091..2408)
                     /gene="sugE"
                     /locus_tag="ACHKYO_RS14720"
                     /old_locus_tag="pgaptmp_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU4429760.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2583..2729)
                     /gene="ecnB"
                     /locus_tag="ACHKYO_RS14725"
                     /old_locus_tag="pgaptmp_002945"
     CDS             complement(2583..2729)
                     /gene="ecnB"
                     /locus_tag="ACHKYO_RS14725"
                     /old_locus_tag="pgaptmp_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU4429761.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2840..2965)
                     /gene="ecnA"
                     /locus_tag="ACHKYO_RS14730"
                     /old_locus_tag="pgaptmp_002946"
     CDS             complement(2840..2965)
                     /gene="ecnA"
                     /locus_tag="ACHKYO_RS14730"
                     /old_locus_tag="pgaptmp_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGU4429762.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(3017..3583)
                     /gene="efp"
                     /locus_tag="ACHKYO_RS14735"
                     /old_locus_tag="pgaptmp_002947"
     CDS             complement(3017..3583)
                     /gene="efp"
                     /locus_tag="ACHKYO_RS14735"
                     /old_locus_tag="pgaptmp_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGU4429763.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3625..4653
                     /gene="epmB"
                     /locus_tag="ACHKYO_RS14740"
                     /old_locus_tag="pgaptmp_002948"
     CDS             3625..4653
                     /gene="epmB"
                     /locus_tag="ACHKYO_RS14740"
                     /old_locus_tag="pgaptmp_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGU4429764.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAKTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            5043..5912
                     /locus_tag="ACHKYO_RS14745"
                     /old_locus_tag="pgaptmp_002949"
     CDS             5043..5912
                     /locus_tag="ACHKYO_RS14745"
                     /old_locus_tag="pgaptmp_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HGU4429765.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(6115..6468)
                     /locus_tag="ACHKYO_RS14750"
                     /old_locus_tag="pgaptmp_002950"
     CDS             complement(6115..6468)
                     /locus_tag="ACHKYO_RS14750"
                     /old_locus_tag="pgaptmp_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HGU4429766.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(6606..8252)
                     /gene="groL"
                     /locus_tag="ACHKYO_RS14755"
                     /old_locus_tag="pgaptmp_002951"
     CDS             complement(6606..8252)
                     /gene="groL"
                     /locus_tag="ACHKYO_RS14755"
                     /old_locus_tag="pgaptmp_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGU4429767.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(8296..8589)
                     /locus_tag="ACHKYO_RS14760"
                     /old_locus_tag="pgaptmp_002952"
     CDS             complement(8296..8589)
                     /locus_tag="ACHKYO_RS14760"
                     /old_locus_tag="pgaptmp_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGU4429768.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            8865..10121
                     /gene="yjeH"
                     /locus_tag="ACHKYO_RS14765"
                     /old_locus_tag="pgaptmp_002953"
     CDS             8865..10121
                     /gene="yjeH"
                     /locus_tag="ACHKYO_RS14765"
                     /old_locus_tag="pgaptmp_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HGU4429769.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLTSEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(10137..10613)
                     /locus_tag="ACHKYO_RS14770"
                     /old_locus_tag="pgaptmp_002954"
     CDS             complement(10137..10613)
                     /locus_tag="ACHKYO_RS14770"
                     /old_locus_tag="pgaptmp_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HGU4429770.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            10950..12386
                     /gene="aspA"
                     /locus_tag="ACHKYO_RS14775"
                     /old_locus_tag="pgaptmp_002955"
     CDS             10950..12386
                     /gene="aspA"
                     /locus_tag="ACHKYO_RS14775"
                     /old_locus_tag="pgaptmp_002955"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HGU4429771.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            12504..13805
                     /gene="dcuA"
                     /locus_tag="ACHKYO_RS14780"
                     /old_locus_tag="pgaptmp_002956"
     CDS             12504..13805
                     /gene="dcuA"
                     /locus_tag="ACHKYO_RS14780"
                     /old_locus_tag="pgaptmp_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HGU4429772.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            13921..14259
                     /gene="cutA"
                     /locus_tag="ACHKYO_RS14785"
                     /old_locus_tag="pgaptmp_002957"
     CDS             13921..14259
                     /gene="cutA"
                     /locus_tag="ACHKYO_RS14785"
                     /old_locus_tag="pgaptmp_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HGU4429773.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            14235..15932
                     /gene="dsbD"
                     /locus_tag="ACHKYO_RS14790"
                     /old_locus_tag="pgaptmp_002958"
     CDS             14235..15932
                     /gene="dsbD"
                     /locus_tag="ACHKYO_RS14790"
                     /old_locus_tag="pgaptmp_002958"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HGU4429774.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITSEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVTNNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILVLPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            15969..16544
                     /locus_tag="ACHKYO_RS14795"
                     /old_locus_tag="pgaptmp_002959"
     CDS             15969..16544
                     /locus_tag="ACHKYO_RS14795"
                     /old_locus_tag="pgaptmp_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4429775.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPDHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            16651..16726
                     /locus_tag="ACHKYO_RS14800"
                     /old_locus_tag="pgaptmp_002960"
     tRNA            16651..16726
                     /locus_tag="ACHKYO_RS14800"
                     /old_locus_tag="pgaptmp_002960"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16684..16686,aa:Phe,seq:gaa)
     gene            complement(16922..17959)
                     /locus_tag="ACHKYO_RS14805"
                     /old_locus_tag="pgaptmp_002961"
     CDS             complement(16922..17959)
                     /locus_tag="ACHKYO_RS14805"
                     /old_locus_tag="pgaptmp_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4429776.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLLPQTENPL
                     VEASVASTEKKKKTLSDNQMLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVLD
                     VLRYTGMRFNQLKHIRVRDVCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLER
                     ARQCGAGDDDYLFDVCRFSEPISADRYQYCPVRAHQTVRSFFRRLSRECGFPVSPHRF
                     RHTLATRLMKYPERNLHLVKSILGHRSLTTTMGYVDVCMESAAQTLEDALSLYTDERT
                     VSKICKGGNKS"
     gene            complement(18027..19508)
                     /locus_tag="ACHKYO_RS14810"
                     /old_locus_tag="pgaptmp_002962"
     CDS             complement(18027..19508)
                     /locus_tag="ACHKYO_RS14810"
                     /old_locus_tag="pgaptmp_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HGU4429777.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGHWGYTGGFGDLTLQ
                     FTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRC
                     WQPGISVPEGVYRFRFRKMPCQGTEGRQLAALMAGQLLPGEAGAWLATVPGALQNLAG
                     AVWHQHPEMALIRTVLQEAMEEVVSPFAAGDVAEVPVAEVVPVLLQSAAISAESVIQP
                     LPVVPSPVEDAENEGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPD
                     KESSIELPEESGCVTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLREGSLS
                     VNSVDSNVHVVSGYVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFF
                     QARLYSDAEHSGRYKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
     gene            complement(19501..20991)
                     /locus_tag="ACHKYO_RS14815"
                     /old_locus_tag="pgaptmp_002963"
     CDS             complement(19501..20991)
                     /locus_tag="ACHKYO_RS14815"
                     /old_locus_tag="pgaptmp_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HGU4429778.1"
                     /translation="MSFNPTDEQYAIINWQGQQLVVNAFSGTGKTSTLVQFARASPDS
                     RMLYLAYNRAIRDEAERKFPFNVECRTSHQLAYARVGRHYRHRLVPGLRITDVARKLN
                     TRYWALARVAGTGLNHFICSADAVPGLHHLPDKDEMRGVPPADALRAVQLLWNEMSNP
                     GGTFPVTHDTYLKLFQLSGADLSHRWDTILFDEAQDASPVTTALVLGQKCRVVLVGDC
                     YQQIYRFRGANNALSHPALKNADRLWLTQSFRFGPAVARMANLLLQREGETREVKGCG
                     GDDEVLLKCHAREHLQGHYTVLSRTVAGVIATALMAAMKGQKVYWVGGIEGYRTGELE
                     DLYWFQVDMPERMHSDRLRRDYRNFEEYKYIAKSTKDVEMNQSLRLLELCFPLPKKLE
                     LLRQYTVTNEQDADITVSTAHRSKGLEWPVVMLDEDFQDITDPLMTEAERHDETCLLY
                     VAVTRALRTLVLNDLMHQLAEEQQKTEGYRGTGYDISGLVQENGHA"
     gene            21223..22899
                     /locus_tag="ACHKYO_RS14820"
                     /old_locus_tag="pgaptmp_002964"
     CDS             21223..22899
                     /locus_tag="ACHKYO_RS14820"
                     /old_locus_tag="pgaptmp_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU4429779.1"
                     /translation="MSEVKKHVSYSLTVAAMLSAAIVCQTAHADVNTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIRKVGEDIV
                     LDGYSVSLGNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGSDTLKRR
                     ITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSGQVITETRARTEG
                     DAAALAAARAHSDENDKRTLVSANTYADQKETAINNRTDGLLTTERQARTAGDTETLA
                     AARTHSDENDKRTLVSANTYADQKETDINNRTDGLLTTERQARTAGDTATLAAARTHS
                     DENDKRTLVSANTYADQKETDINNRTDGLLTTERQARTAGDTATLAAARTHSDENNKR
                     TLISANTYADQKEIAINNRTDGLLMTERQARIAGDAETLATAEAYSDAGDSLTLKQAT
                     AYSDISTRRALSEANRYTDNKFRNLSNKIERAEKRLNAGIAGVTAISSIPYVAENSFS
                     YGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDSSHNTAIGAGVAGGW"
     gene            complement(23825..24067)
                     /locus_tag="ACHKYO_RS14825"
                     /old_locus_tag="pgaptmp_002965"
     CDS             complement(23825..24067)
                     /locus_tag="ACHKYO_RS14825"
                     /old_locus_tag="pgaptmp_002965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429780.1"
                     /translation="MMMARDITAYHLNSVHISARRFPGIFQWATGMQTKNLPLTNSLL
                     QNDYAESIFVFNGERDVSEMPLPEEMLLTGNAESER"
BASE COUNT         6166 a         6391 c         5744 g         5949 t
ORIGIN      
        1 caatattcta tgaacttgat ggcgagtggt atcacgagat ttgcaggaat gccattaaac
       61 gaggcggaca acgaggtgaa catctgaggg ctaatatcga cagagccagt gtgcataagt
      121 tccgacagtt tcgttacatc cggtttctga acaagagggc cagaaaacgc ctcaacacaa
      181 agctattcaa agtccagcct tacccaaaac cacaaacagt taaaactggt ttgaaagaga
      241 gtgaatgagc gcttaaaaac tcattctaaa actcaaaaga aaagcgccga tttagtatca
      301 aataagttgc gtatcggcgt ttttcatcgg tatcaaatga aatgcaaatc ggtatcaaaa
      361 aaatcgccgc gctatactct tgctccacat ttgctgcccc tcaacaaatc aacgatattg
      421 tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg gcggtggcgg
      481 taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc
      541 agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg
      601 tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt aagcgatccc acaacaaaat
      661 actggcctga acttaccgct aaacagtgga atgggatcac actattacat ctcgcaacct
      721 acactgctgg cggcctgcca ttgcaggtgc cggatgaggt gaaatcctca agcgacttgc
      781 tgcgcttcta tcaaaactgg cagcctgcat gggcgccagg aacacaacgt ctgtatgcca
      841 actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc
      901 aggcgatgca aacacgtgtc ttccagccac tcaaactcaa ccatacgtgg attaatgtac
      961 cgcccgcaga agaaaagaat tacgcctggg gatatcgcga gggcaaggca gttcatgttt
     1021 cgccaggggc gttagatgct aaaacttatg gtgtgaagtc gaccattgaa gatatggcct
     1081 gctgggtacg aagcaatatg aatccccgtg atatcaacga caaaacactt cagcaaggga
     1141 tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc ctgggctggg
     1201 aaatgctgga ctggccggta aatcctgaca gcatcattaa cggcagtggc aataaaattg
     1261 cactggcagc acaccccgta aaagcgatta cgcccccaac tcctgcagta cgcgcatcat
     1321 gggtacataa aacaggggca accggcggat ttggtagcta tgtcgcgttt attccagaaa
     1381 aagagctggg tatagtgatg ctggctaaca aaaactatcc caatccagcg agagtcgccg
     1441 ccgcctggca gattctcaac gctctacagt aaaatttcat cgggtccgaa ttttcggacc
     1501 ttttctgcgc ttttccttgc tgtcatctac acttagaaaa aaaccagtaa ggaaacattt
     1561 atgcgcctgc tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt
     1621 tctcctacgc cgccgcgtgg cgtaacagta gtgaataatt tcgacgccaa acgttatctc
     1681 ggtacctggt atgagattgc ccgttttgat caccgttttg aacgtggact ggaaaaagtc
     1741 accgcaacat acagcctgcg tgatgacggc ggcctgaatg tcattaataa aggctataac
     1801 cctgacagag gaatgtggca gcagagtgaa gggaaagcgt actttaccgg cgcaccaact
     1861 cgcgctgcgc tgaaagtgtc attctttggt cctttctatg gcggttataa cgttattgca
     1921 ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg accgcgacta cctgtggata
     1981 ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc
     2041 cgggaagggt ttgatgtcag taagtttatt tgggtacagc agcccggtag ttagtgagtg
     2101 ctgagtttca gaccaataat ccccaatacg attaacgcca gactcgccag gcgcatcggg
     2161 ttagcggact caccgagcag cacaatgccg gtgatagccg cgccgactgc gccaataccc
     2221 gtccacacgg cataagccgt ccctactggt aacgatttca tcgcccaggc aagtagcgcc
     2281 atactgacta tcatcgccgt cacggtaata acactcggcg tcaaacggct aaagccgtgg
     2341 gtatatttca ggccaacggc ccataccact tccagcagac cagcaataac taagataatc
     2401 caggacatat caggctccgg agaattgggg ccgtccccgg tgaaaagagg cgtttgcagg
     2461 tcgtcctgca aagctgatgg ttgaacggat attttgccag gtggcagggg aatttcaact
     2521 attacctttg tgactatcgc aaaaaaacaa acggcacgac ataactgtcg taccgttatt
     2581 gcttattgct gcgctttcgt tgctgcacca gaaatcgcgt taccgccatc agaaatgtct
     2641 tcaccaacgc cacgcgtggt gttgcaggca gttaatactg ttgaaagcac cagaacagaa
     2701 aagatcgctg caattgtctt cttcaccata acgtcttcct tttaggtaat gttatttatg
     2761 tttgcctata gcaaattaat aatagacaac atcgccaaaa atgatggaaa tctgatgatt
     2821 tttcggaaga gaagaaaaat tagctggcag cgcgggagat ggagttgccg agatgtttga
     2881 tgtcttcgcc gaaaccgcga gcggtgttac agcccgtgag cagcgtgctg gcaagcaaaa
     2941 caagaacgat aaggcgtttc atcatctctg acctgaataa gtgatggtgc agcctgcagg
     3001 ccgcaccaca accgcattac ttcacgcgag agacgtattc accagagcgg gtatccactt
     3061 tgatgacttc gccgatttgt acgaacagcg gaactttaac cacagcgcca gtagacaggg
     3121 tagccggttt gccgccagta cctgcggtat cacctttcag gcccggatcg gtatcaacga
     3181 tttccagttc aacgaagttc ggcggagtaa cggagatcgg ctgaccattc cacagagtta
     3241 cgatacactc tgcctgatcc agcagccatt tagcgttgtc gccaattgct tttgcatcag
     3301 cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca gaactcaccg tcgttgtaca
     3361 ggtaagtcag gttcatatcg acaacatcag cgccttcagc ggaatcagta gatttgaagg
     3421 ttttttctac gcgagtaccg gtcagcagac gacgcagttt aacgcgagca aatgcctggc
     3481 ctttacccgg ttttacgaat tcactcgctt caaccgcgta aggttcgccg tctaacatga
     3541 ttttaagacc agcacgaaaa tcgttgctat agtacgttgc cataaggccc tctgaaattt
     3601 gttaattggt agctaagcca caaaatggcg catattgtaa ccctaaatac cccatccaga
     3661 gaagattggt taacgcaact tgccgatgtt gtgaccgatc ctgatgaact tctgcgtctt
     3721 ttgaatatag acgcggacga aaaactgttg gccggacgca gcgccaaaaa gctgtttgcc
     3781 ctgcgtgtgc cccgctcatt tatcgatcgc atggagaaag gcaatccgga cgatcctctt
     3841 ttgcgtcagg tacttacctc gcaagatgag tttgtcgtcg cgcccggatt ctccaccgac
     3901 ccactggaag aacagcacag cgtagtgcct ggtttgttgc ataaatacca caaccgggcg
     3961 cttttgctgg tcaaaggcgg ctgcgcggta aattgccgtt attgcttccg tcgccacttc
     4021 ccctatgccg aaaatcaggg caacaagcgt aactggcaga ctgctcttga gtatgttgct
     4081 gctcatccgg aactggacga gatgattttc tccggcggcg atccgctgat ggcgaaagat
     4141 cacgagctgg actggttgct cacacaactg gaagccatcc cgcatatcaa acgtctgcgg
     4201 attcacagcc gtctgccgat tgtgatcccg gcacgtatca ccgaggcgct ggttgaacgc
     4261 tttgcccgtt ctacgctgca aatcttgctg gtgaatcaca tcaatcatgc caatgaggta
     4321 gatgaaacgt tccgtcaggc gatggctaaa ttgcgccgtg tcggtgtcac cctgcttaac
     4381 cagagcgttc tgttacgtgg tgtgaacgat aacgcaaaaa cgctggcaaa cctgagtaat
     4441 gcgttgttcg atgccggcgt aatgccgtat tacctgcatg tgctcgataa agtacagggc
     4501 gcggcgcatt ttatggtgag tgatgacgaa gcacggcaga ttatgcgtga gttgctgaca
     4561 ctggtgtcgg gttatctggt gccgaaactg gcgcgagaaa tcggcggcga acccagcaaa
     4621 acgccgctgg atctccagct acgccagcag taactgatta aacagggaag ttaaacatta
     4681 gcctgatgcg ctacgcttat caggcctaca ttgtgcattg aaattgaatg gatttgcaat
     4741 gaactgtagg ccggataagg cgtttacgcc gcatccggca agagcaacaa acgcaagtac
     4801 agcaacctgt cattaaactg ggaaatttaa tctttgcccg ttgcataacc tttaattatt
     4861 catatacatt gctgttgtta ctctttatat agccaacacc aataattaac ctttcttttc
     4921 tgtttatata gagtgagtac ggctatttat gttattttaa caactattac taccaagccc
     4981 atttatctgg cttataaatt aaaattaatt atttaattga gaaatggtta gggagaacct
     5041 acatggccat aagcatcaag ggtgttaata ccggtgttat tcgcaagagt aataatttca
     5101 ttgcactggc gctgaaaatt aaagaacctc gcaataaaga atcgttgttc tttatgtctg
     5161 tcatggaact gcgcgacctg ctgattgcgc tggaaagtcg tatgcatcaa aagcataaac
     5221 tggatgctgc tgcacgcctt cagtatgagc aagctcgcga taaagtcatt aagaaaatgg
     5281 cagaaaacat cccagaaatt ctggttgatg agcttaaaaa tgccgatatt aatcgtcggg
     5341 ttaacacgct ggaacttacc gataatcaag gcgaaaatct taccttcgta ttaacgctac
     5401 atgacggtag caaatgtgag ttagtcgtca atgagttgca gattgaaatg ctggcgcgag
     5461 caatcattca tgcaattaat aatgcggaaa tgcgtgaact ggcattacgt attacctcac
     5521 tgttagattt cctgccgtta tatgacgttg actgccagga aaatggcaat ctggaatatg
     5581 acacctattc ccaaccggaa tggaaacata acctgttcga ccattatctg gcggtgcttt
     5641 accgctttaa agatgaaagt ggcaaagaac aatttagcgg cgcagtggtg aaaacgcgtg
     5701 aagcaacgcc gggtaaagaa atagaggcga tcactcgccg aatgcttgat ttcagccaac
     5761 gactgaaaaa actcgcgggt gtgccatgtc aggtctatgt tcgcaccgta gcggcgaata
     5821 acgcccaacc gctaacccag gatcaatgct tacgcgcgct gcatcactta cgtgttcaat
     5881 ccaccagcaa aactgcacca caggcgaaat aatctcatct ttgctaatgc gaggctggag
     5941 ccatccagcc tgaaatgact gataacgtga tcgttggtca tgccggatgc ggcgtaaacg
     6001 ccttatccgg cctacaaatt ctcaagcatt caatcagttg caatgcctgc tgtaggcctg
     6061 ataagcgaag cgcatcaggc aatgatacgt ttgtcaccca gcctctggat cacatcagtt
     6121 cgggcactta taaacctgac cgataatctg gctatccgtc gggacaaaac tggacaacat
     6181 tccctgcgac gggctgctga tgccataaat cacgttaccg cccattgcag ccgcctggtt
     6241 gcgcagatcg tttgctgcgc cgcgcataga accgccctct tctccgtgtt gcccggaaag
     6301 ccagttgctt tgcttacctg tcgcagtacc aatcagctgg cactctgcgc ctggctgctc
     6361 gtccacaatg cgtacactct gaccggcagc actcaattcg ttgctggagc tacaacccgc
     6421 catcagtagc gcggcaccga caatccctgc taagtatttt acgtgcatgt tattccccat
     6481 aatcaatgag ctggacgcca ctcgcgtcgt ccgtgtctga atcttatact agaaagatga
     6541 ccaaaagaaa aacccccaga catttctgcc cgggggtttg tttatttctg cgaggtgcag
     6601 ggcaattaca tcatgccgcc catgccaccc atgccgccca taccgccagc agcgcctaag
     6661 tcagctgcat catttttcgg caggtcggta accatgcact cggtggtgat catcaggcca
     6721 gccacagaag ccgcgtactg cagagcagaa cgggttactt tggttgggtc caggataccc
     6781 atgtcgatca tgttgccgta ttcttcggtt gctgcgttgt aaccgtagtt gccgtcgccg
     6841 cctttaacgg tgttagcaac aacagacggt tcttcgccgc agttcaggac gatctgacgc
     6901 agcggagctt ccattgcacg cagtgcaact ttgataccca cgttctggtc ttcgttctga
     6961 ccacgcaggt cagccagttt agacgctacg cggatcagcg caacaccacc accagcaacc
     7021 acgccttctt ctaccgcagc acgggtcgcg tgcagggcgt cttcaacgcg tgcttttttc
     7081 tctttcattt caacttcggt agcagcacct actttgataa ctgcaacgcc gcctgccagt
     7141 ttcgctacgc gttcctgcag tttttcacgg tcgtagtcag aagttgcttc ttcaatctgc
     7201 tgacggatct gagcaacacg gccctggatt gcagcttctt cacccacgcc atcgatgata
     7261 gtggtggtgt ctttgttgat cacaacacgt ttagcctgac ccaggtcttc cagggttgct
     7321 ttttccagct ccataccgat ctcttcagag attacggtac cgccagtcag ggttgcgata
     7381 tcctgcagca tagctttacg acgatcgccg aagcccggag ctttaaccgc agcaactttc
     7441 acgatgccac gcatggtgtt aacaaccaga gttgccagcg cttcgccttc aacatcttca
     7501 gcgatgatca gcagcggttt acctgctttg gcaacggctt ccagaaccgg cagcatttcg
     7561 cggatgttgg agattttctt gtcagccagc aggatgaacg ggctttccag ttctactgcg
     7621 ccagtttccg gcttgttgat gaagtaagga gacaggtagc cacggtcgaa ctgcatacct
     7681 tcaaccacgt ccagttcgtc ctgcagaccg gtaccgtctt caacggtgat aacgccttct
     7741 ttaccgactt tgtccatcgc ttcagcgatc agtttaccta cggtttcgtc ggagttagcg
     7801 gagatggtac caacctgagc aatcgcttta gagtcagagc acggtacgga cagcgctttc
     7861 agttcttcaa ctgcagcggt aacggctttg tcgataccac gtttcaggtc catcgggttc
     7921 atgcccgcag caacagcttt cagaccctca gtgatgatag cctgagccag tacagttgca
     7981 gtggtggtac cgtcgcctgc agcgtcgttc gctttagagg caacttcttt caccatctgc
     8041 gcacccatat tttcgaactt gtcttccagt tcgatttcac gagcaacgga aacaccatct
     8101 ttggtgatgg tcggtgcacc gaaagattta tccagaacta cgttacggcc tttcggaccg
     8161 agggtaactt tcactgcatc tgccagtacg tttacgccgc gcagcatttt cacacgagcg
     8221 tcgttaccga attttacgtc tttagctgcc attatcttta ttccttaaat tcgtatgttc
     8281 agtgtcgtgc gaggattacg cttcaacaat tgccagaatg tcgctttcgg acatgatcaa
     8341 cacttcttca ttgtcgatct tctcagattt cacaccgtag ccatcgttga aaataacgat
     8401 gtcgccaact ttcacatcca gcggcttcac ttcgccattt tcaaggatac ggccattgcc
     8461 gacagccagc acttcgccgc gggtggattt agccgctgca gagccggtca gaacgatgcc
     8521 gccagcagat ttagtttcaa cttctttacg cttgacgatc acgcgatcat gcaatggacg
     8581 aatattcatt gataactctc ctttgagaaa gtccgtatct gttatgggtg acgccggagc
     8641 ttacgtggtt tcccggctgg tgaccagaga aatggggatg aggattcgcc ccttcaaggg
     8701 ggaaaaagaa aaaaaattct gatcagcaca aaatcgggtg aaaaccctga ttcacctcac
     8761 atttcatcgc aatttcttca tcgccgtcat aagcgaatct gattgtgcta ccatcgaaaa
     8821 tctacgcagt tgcccaaaat ttgggcgcaa tcggatatca acccatgagt ggactcaaac
     8881 aagaactggg gctggcccag ggcattggcc tgctatcgac gtcattatta ggcactggcg
     8941 tgtttgccgt tcctgcgtta gctgcgctgg tagcgggcaa taacagcctg tgggcgtggc
     9001 ccgttttgat tatcttagtg ttcccgattg cgattgtgtt tgcgattctg ggtcgccact
     9061 atcccagcgc aggcggtgtc gcgcacttcg tcggtatggc gtttggttcg cgacttgagc
     9121 gagtcaccgg ctggttgttt ttatcggtca ttcccgtggg tttgcctgcc gcgctacaaa
     9181 ttgccgccgg gttcggccag gcaatgtttg gctggcatag ctggcaactg ttgttggcag
     9241 aactcggtac gctggcgctg gtgtggtata tcggtactcg cggtgccagt tccagtgcta
     9301 atctacaaac cgttattgcc ggacttatcg tcgcactgat tgtcgctatc tggtgggcgg
     9361 gcgatatcaa acctgcgagt atccccttcc ccgcgccagg aaatatcgaa cttaccgggt
     9421 tatttgctgc gttatcagtg atgttctggt gttttgtcgg tctggaagca tttgcccatc
     9481 ttacctcgga atttaaaaat ccagagcgtg attttcctcg cgcattgatg attggtctgc
     9541 tgctggcagg attagtctac tggggctgta cggtagtcgt cttacacttc gacgcctatg
     9601 gtgaacaaat ggcggcggca gcatcgcttc caaaaattgt agtgcaactg ttcggtgtag
     9661 gagcgttatg gattgcctgc gtaattggct atctggcctg ctttgccagt ctcaacattt
     9721 atatacagag cttcgcccgc ctggtctggt cgcaggcgca acataatcct gaccactacc
     9781 tggcacgcct ctcttctcgc catatcccga ataatgccct caatgcggtg ctcggctgct
     9841 gcgtggtgag cacgttggtg attcatgctt tagagatcaa tctggacgct cttattattt
     9901 atgccaatgg catctttatt atgatttatc tgttatgcat gctggcaggc tgtaaattat
     9961 tgcaaggacg ttatcgacta ctggcggtgg ttggcgggct gttatgcgtt ctgttactgg
    10021 caatggtcgg ctggaaaagt ctctatgcgc tgatcatgct ggcggggtta tggctgtttc
    10081 tgccaaaacg aaaaacgccg gaaaatggca taaccacata atccggcgtt tcgacattaa
    10141 tcctggcgat cgtctttatg atcaaggcgg tcgcgctcat catcctttcg ctggtactca
    10201 ccatcaaaag tattaccgcc accggtcccg gcgctaaaac cgccgcctgg catacgagaa
    10261 aagcgcaaat gcggcatcaa cttcactgtc agatgctttt gcaccggcgg taataaaagc
    10321 agaagaccga ggaagtcggt gaaaaagccc ggtaataaaa gcagcaaacc ggcaatgatc
    10381 agcgaaacac ttttaatcat ctccgccgct gggttttcac ccgccgccat tttttgctgc
    10441 atcagcacga aattcttaaa gccctggtta cgtaccagtg acatcccgat aactgacgtg
    10501 aatataacca gcacgagggt cagcaatacc cccaatacat gggcaacctg aataaagatt
    10561 gaaatctcaa tatagacata aaggaaaatg gcaataaaag gtaaccagcg caaaggtttc
    10621 tcctgtaata gcagccggta aaacccggct acctgaatgg gttgcgaatc gcgttcagct
    10681 tatattgtgg tcattagcaa aatttcaaga tgtttgcgca actatttttg gtagtaatcc
    10741 caaagcggtg atctatttca caaattaata attaaggggt aaaaaccgac acttaaagtg
    10801 atccagatta cggtagaaat cctcaagcag catatgatct cgggtattcg gtcgatgcag
    10861 gggataatcg tcggtcgaaa aacattcgaa accacatata ttctgtgtgt ttaaagcaaa
    10921 tcattggcag cttgaaaaag aaggttcaca tgtcaaacaa cattcgtatc gaagaagatc
    10981 tgttgggtac cagggaagtt ccagctgatg cctactatgg tgttcacact ctgagagcga
    11041 ttgaaaactt ctatatcagc aacaacaaaa tcagtgatat tcctgaattt gttcgcggta
    11101 tggtaatggt taaaaaagcc gcagctatgg caaacaaaga gctgcaaacc attcctaaaa
    11161 gtgtagcgaa tgccatcatt gccgcatgtg atgaagtcct gaacaacgga aaatgcatgg
    11221 atcagttccc ggtagacgtc taccagggcg gcgcaggtac ttccgtaaac atgaacacca
    11281 acgaagtgct ggccaatatc ggtctggaac tgatgggtca ccagaaaggt gaatatcagt
    11341 acctgaaccc gaacgaccat gttaacaaat gtcagtccac taacgacgcc tacccgaccg
    11401 gtttccgtat cgcagtttac tcttccctga ttaagctggt agatgcgatt aaccaactgc
    11461 gtgaaggctt tgaacgtaaa gctgtcgaat tccaggacat cctgaaaatg ggtcgtaccc
    11521 agctgcagga cgcagtaccg atgaccctcg gtcaggaatt ccgcgctttc agcatcctgc
    11581 tgaaagaaga agtgaaaaat atccaacgta ccgctgaact gctgctggaa gttaaccttg
    11641 gcgcaacagc aatcggtact ggtctgaaca cgccgaaaga gtactctccg ctggcagtga
    11701 aaaaactggc tgaagtcact ggcttcccat gcgtaccggc tgaagacctg atcgaagcga
    11761 cctctgactg cggcgcttat gttatggttc acggcgcgct gaaacgcctg gctgtgaaga
    11821 tgtccaaaat ctgtaacgac ctgcgcttgc tctcttctgg cccacgtgcc ggcctgaacg
    11881 agatcaacct gccggaactg caggcgggct cttccatcat gccagctaaa gtaaacccgg
    11941 ttgttccgga agtggttaac caggtatgct tcaaagtcat cggtaacgac accactgtta
    12001 ccatggcagc agaagcaggt cagctgcagt tgaacgttat ggagccggtc attggccagg
    12061 ctatgttcga atccgttcac attctgacca acgcttgcta caacctgctg gaaaaatgca
    12121 ttaacggcat cactgctaac aaagaagtgt gcgaaggtta cgtttacaac tctatcggta
    12181 ttgttactta cctgaacccg ttcatcggtc accacaacgg tgacattgtg ggtaaaatct
    12241 gtgccgaaac cggtaagagt gtacgtgaag tcgttctgga acgcggtttg ttgactgaag
    12301 cggaacttga cgatattttc tccgtacaga atctgatgca cccggcttac aaagcaaaac
    12361 gctatactga tgaaagcgaa cagtaatcgt acagggtagt acaaatagag aaggcacgtc
    12421 agatgacgtg ccttttttct tgtgagtagt aacttaaaaa taacaatcta atatcaactt
    12481 gttaaaaaac aaggaaggct aatatgctag ttgtagaact catcatagtt ttgctggcga
    12541 tcttcttggg cgccagattg gggggaatag gtattggttt tgcaggcgga ttgggggtgc
    12601 tggttcttgc cgctattggc gttaaacccg gtaacatccc gttcgatgtc atttccatta
    12661 tcatggcggt tatcgccgct atttctgcca tgcaggttgc tggcggtctg gactatctgg
    12721 ttcatcagac agaaaagctg ctgcgccgta acccgaaata catcacgatc ctcgcaccga
    12781 tcgtgaccta tttcctgact atctttgctg gtactggcaa catctctctg gcgacactgc
    12841 cagttatcgc tgaagttgcg aaggaacaag gcgttaaacc ttgccgtccg ctgtctactg
    12901 cagtggtatc cgcgcagatt gcgatcaccg catcgccaat ctcagcggca gtggtttaca
    12961 tgtcttccgt gatggaaggt catggcatca gctacctcca tctgctctcc gtggtcatcc
    13021 cgtccaccct gctggcggtt ctggtgatgt ccttcctggt cactatgctg ttcaactcca
    13081 aactctctga cgatccgatt tatcgcaagc gtctggaaga gggcctggtt gaactgcgcg
    13141 gtgaaaagca gattgaaatc aaatccggtg caaaaacgtc cgtctggctg ttcctgctgg
    13201 gcgtagttgg cgtggttatc tatgcaatca tcaacagccc aagcatgggt ctggttgaaa
    13261 aaccgctgat gaacaccacc aacgcaatcc tgatcatcat gctcagcgtt gcaactctga
    13321 ccaccgttat ctgtaaagtc gataccgaca acatcctcaa ctccagcacc ttcaaagcag
    13381 gtatgagcgc ctgtatttgt atcctgggtg ttgcgtggct gggcgatact ttcgtttcca
    13441 acaacatcga ctggatcaaa gataccgctg gtgaagtgat tcagggtcat ccgtggctgc
    13501 tggccgtcat cttcttcttt gcttctgctc tgctgtactc tcaggctgca accgcaaaag
    13561 cactgatgcc gatggctctg gcactgaacg tttctccgct gaccgctgtt gcttctttcg
    13621 ctgcggtgtc tggtctgttc attctgccga cctacccgac gctggttgct gcggtacaga
    13681 tggatgacac gggtactacc cgtatcggta aattcgtctt caaccatccg ttcttcatcc
    13741 cgggtactct gggtgttgcc ctggccgttt gcttcggctt cgtgctgggt agcttcatgc
    13801 tgtaatgacc aatcgcgggg cgttcacgcc ccgctttctt tcccgccgac taacatcctt
    13861 cccccgtccg ttgtatagtg acctctctct tgcggttcca tctgttcttg cgaggtgttt
    13921 atgcttgatg aaaaaagttc gaataccgcg tctgtcgtgg tgctatgtac cgcaccggat
    13981 gaagcgacag cccaggattt agccgccaaa gtgctggcgg aaaaactggc ggcctgcgcg
    14041 accttgatcc ccggcgctac ctctctctat tactgggaag gtaagctgga gcaagaatac
    14101 gaagtgcaga tgattttaaa aactaccgta tctcaccagc aggcactgct ggaatgcctg
    14161 aagtctcatc atccatatca aaccccggaa cttctggttt tacctgttac acacggagac
    14221 acagattacc tctcatggct caacgcatct ttacgctgat cctgctactt tgcagcactt
    14281 ccgtttttgc cggattattc gacgcgccgg gacgttcaca atttgtcccc gcggatcaag
    14341 catttgcttt tgattttcag caaaaccaac atgacctgaa tctgacctgg cagatcaaag
    14401 acggttacta cctctaccgt aaacagatcc gcattacgtc ggaacacgcg aaaattgccg
    14461 acgtgcagct gccgcaaggc gtctggcatg aagatgagtt ttacggcaaa agcgagattt
    14521 accgcgatcg gctgacgctt cccgtaacca tcaaccaggc gagtgcggga gcgacgttaa
    14581 ctgtcaccta ccagggctgt gctgatgccg gtttctgtta tccgccagaa accaaaaccg
    14641 ttccgttaag cgaagtggtc accaacaacg cagcgtcaca gcctgtgtct gttccgcagc
    14701 aagagcagcc caccgcgcaa ttgccctttt ccgcgctctg ggcgttgttg atcggtattg
    14761 gtatcgcctt tacgccatgc gtgctgccaa tgtacccact gatttctggc atcgtgctgg
    14821 gcggtaaaca gcggctttcc actgccagag cattgttgct gacctttatt tatgtgcagg
    14881 ggatggcgct gacttacacg gcactgggtc tggtggttgc cgccgcaggg ttacagttcc
    14941 aggcggcgct acagcaccca tacgtgctca ttggcctcgc tatcgtcttt accttgctgg
    15001 cgatgtcaat gtttggcttg tttactctac aactcccctc ctcgctgcaa acgcgcctca
    15061 cgctgatgag caatcgccaa cagggcggct cacctggcgg tgtgtttgtt atgggggcga
    15121 ttgccggact gatctgttca ccatgcacca ccgcaccgct tagcgcgatt ctgctgtata
    15181 tcgcccaaag cgggaacatg tggctgggcg gcggcacgct ttatctctat gcgttgggca
    15241 tgggcctgcc gctgatgctc attaccgtct ttggtaaccg cttgctgccg aaaagcggcc
    15301 cgtggatgga acaagtcaaa accgcgtttg gttttgtgat cctcgtactg ccggtcttcc
    15361 tgctggagcg agtgattggt gatatatggg gattacgctt gtggtcggcg ctgggtgtcg
    15421 cattctttgg ctgggccttt atcaccagcc tacaggctaa acgcggctgg atgcgcgtgg
    15481 tgcaaataat cctgctggca gcggcattgg ttagcgtgcg cccacttcag gattgggcat
    15541 ttggtgcgac gcataccgcg caaactcaga cgcatctcaa ctttacacaa atcaaaacgg
    15601 tagatgagtt aaatcaggcg ctcgttgaag ccaaaggcaa accggtgatg ttagatcttt
    15661 atgccgactg gtgcgtcgcc tgtaaagagt ttgagaaata caccttcagc gacccgcagg
    15721 tgcaaaaagc gttagcagac acggtcttac ttcaggccaa cgtcacggcc aacgacgcac
    15781 aagatgtggc gctgttaaag catcttaatg tccttggcct accgacaatt ctcttttttg
    15841 acggacaagg ccaggagcat ccacaagcac gcgtcacggg ctttatggat gctgaaacct
    15901 tcagcgcaca tttgcgcgat cgccaaccgt gaacgacact tgcagtggga aagacggagg
    15961 aaaatagcgt gcaacgtgaa gatgtactgg gagaagccct gaaattatta gaattacaag
    16021 ggattgccaa caccacgctg gagatggttg ctgaacgtgt ggattatcca ctggacgagc
    16081 tacgccgctt ctggccagac aaagaggcaa tcctctacga tgcgctgcgt tatcttagcc
    16141 aacagataga tgtctggcgt cgtcagctga tgctggacga aacgcaaacc gccgaacaaa
    16201 agctgctggc acgttatcag gcgttatcgg agtgcgttaa aaacaaccgc tatccgggct
    16261 gtctatttat cgctgcctgt acgttttatc ccgatcctga ccaccctatt catcaactgg
    16321 ccgatcagca aaaaagcgcg gcctacgatt tcacccacga actgttaacc acactggaag
    16381 ttgacgatcc ggcgatggta gcaaagcaga tggaactggt gctggaaggc tgtttaagcc
    16441 gaatgctggt gaatcgtagc caggcggatg tcgacactgc acatcggctg gcggaagata
    16501 tcctgcgctt cgcccgctgc cgccagggtg gtgcattgac ctgacagaaa cacagaaaag
    16561 aagcgatttg ccgcaatctt aagcagttga atcgctttta ctgaaattag gttgacgaga
    16621 tgtgcagatt acggtttaat gcgccccgtt gcccggatag ctcagtcggt agagcagggg
    16681 attgaaaatc cccgtgtcgg tggttcgatt ccgcctccgg gcaccatata ccagaacacg
    16741 ctggttaagg gatgagatcc ttgacgggcg gttaatacag gatgcagcat caaagggtct
    16801 ccgtttgtgt aagttgtggt tacactgatt gagttcggta tcgtatcctc aaacagggtg
    16861 tcttttgcgg agacgccctg ttctcgttaa cactgtatgc ttacatcgct gattcagcct
    16921 gtcaagattt attcccccct ttgcaaatct tgctcactgt gcgctcatct gtatacaaac
    16981 tcagggcatc ttccagtgtc tgtgctgcag actccataca gacatcaaca taccccatgg
    17041 tcgttgtaag actcctgtgt cccagtatgg atttgaccag atgtaaatta cgttcaggat
    17101 atttcatcag tctggttgcc agcgtatgtc tgaaacgatg tggtgaaaca ggaaaaccac
    17161 attctcgcga aagtcgccgg aaaaatgaac gtacggtctg atgcgcccga acgggacagt
    17221 actgatatct gtcggcagag atcggctcac tgaaacgaca gacgtcaaaa agatagtcat
    17281 catcacctgc cccgcactga cgggcccgct ccagcagttt ttccatcgat accttcaggg
    17341 gagccaccag aggaacgccc cattccctgt gagttttact tccctccagc ttaagatcaa
    17401 ttatgctttc atccggacag acatccctca cacggatatg cttcagttga ttaaagcgca
    17461 ttccggtata acgcagaaca tccagaactg tcagccagta ccaggcaggg tatatagcat
    17521 tgcgtcgacg actattcacc atgttctgct cttccgcttc tgcaaacctc tgcacaacca
    17581 ggtaaaccct gagcatctga ttgtcactca gtgtcttttt ctttttttca gtactcgcaa
    17641 ctgatgcttc caccaacggg ttttctgtct gaggcagcag tttcatcttg atcccgaatc
    17701 ccataattgc ccgcaagtgg gccaccttgt tattccaggt cacctccctg cgcttctgct
    17761 ctttcaggac ataacgccgg tatcgctgaa catcacggac agtcacctcc cccggcaaaa
    17821 tatcaccgat aaattccctg aataccctga caactttgca gtagctccac tctgtatccg
    17881 gccgaagatt ctttgaaaaa aagtactctt ccagcagttg ttcccaggtg atttttgtga
    17941 cggaatcgtc attctgcatt atatattccc cttgtttact gtgctcccgc actctccggt
    18001 caaattctgc acatcatcgc ctttactcac ccgaaaacaa taagcgggct gtctccggga
    18061 accgtaatat tcccgtacag aaacctgccc ttaaccagat atcccgaaac cattttgtaa
    18121 cgtccggaat gttctgcgtc actataaaga cgggcctgaa aaaaacgctc tcctgaacgt
    18181 accttatgga tacctgcccg ttcaaagagc gactgaacat aacgacgatc ctgcccggaa
    18241 ccactctcct tcagaaaacg ataaaaaatc cccggggcac acagaaaaac atatccggaa
    18301 acaacatgaa catttgaatc aacgctgtta accgaaagtg acccctccct cagcccctct
    18361 tttaaccatc tgataaattc actctctacc ctgctatcct ctgtcaggga aaactctgcc
    18421 gttttttcca ctgtttccgg gatacggttt ccctgatttt ctgttacaca tccgctctct
    18481 tccggtaact caatactgct ttctttatcc ggcggcacgc ctgacgctat ctgccctccc
    18541 tcatccgaca aatcagcagc cgaaaacagc gtaaggagca ttccggtatc agcatcagtt
    18601 tcgttcccct catccacctc ctgttccgct gtccggccct cattttcggc atcctccact
    18661 ggcgacggta caaccggcaa cggctgaatg acgctttctg ctgaaattgc tgctgactgc
    18721 agcagtaccg gaacaacctc agccaccgga acttcggcca catctcctgc agcgaaggga
    18781 ctcacgactt cctccatagc ttcctgtaat actgtacgta tcagcgccat ttcaggatgc
    18841 tgatgccaga ctgctccggc aagattctgt aatgcccccg ggacagtggc aagccaggct
    18901 cccgcctctc cgggcagcag ttgtcccgcc atcagggctg ccagttgccg tccctccgtt
    18961 ccctggcagg gcatcttcct gaaccggaac cggtatacgc cctccggtac agatatcccc
    19021 ggctgccagc atctgccgtc ggcctgttcg ccttccagtt ccgccagcaa cggcaggtga
    19081 taaaacagag ccgaccagaa caccaccatg ttccacacac tcccctgcgc ggcctgctct
    19141 tctgctgtcg ctcccggtgg aaacattctg cctctggcca gccggacagc atacgtcgtg
    19201 aactgcaggg tcaggtcacc aaaaccaccg gtgtaacccc agtgaccttc tgctgtggcc
    19261 ggcagattct gactgacaga caacaactga tgcacaggat caagacaaag gcgctgatac
    19321 atatccaccg gcatggaact gttttcccat aactgtttca gacacctttt cctgaacggt
    19381 gtggatacca gtacatcagc cggcaatggt gtaaaatacc cctccggcac cgtttccccg
    19441 ggaatatccg tccggacatc agagcattct gtcttcacag gaaataacct tttcaggcgt
    19501 tcaagcatgc ccattctcct gaaccagacc tgagatgtcg tacccggtcc cccggtaacc
    19561 ttcagttttc tgctgttctt ctgccagttg gtgcatcagg tcattcagta ccagcgtcct
    19621 gagggcccgg gtgacagcca catataacag acaggtttca tcatgacgct cggcctccgt
    19681 catcagcgga tccgtgatat cctggaaatc ctcatccagc attacaaccg gccactccag
    19741 ccccttgctc cggtgtgccg ttgataccgt gatatctgca tcctgctcat tcgttaccgt
    19801 atactgccgg agaagttcca gttttttcgg taacggaaaa cacaactcca gaagccgaag
    19861 ggactgattc atctccacat ccttcgtgga ttttgcaata tatttatatt cttcaaaatt
    19921 cctgtagtcc cggcgcagcc tgtctgaatg catccgttcc ggcatatcaa cctggaacca
    19981 gtagagatcc tcaagttccc cggtcctgta accttcgatt ccccccaccc agtaaacctt
    20041 ttgccctttc attgccgcca tcagcgcagt agcaatcaca cccgcgacag tacgactgag
    20101 taccgtgtaa tgcccctgca gatgttcacg cgcatggcat ttcagcaata cctcatcatc
    20161 accaccacaa cctttcacct ctcgtgtttc accttcacgc tgcagaagca gatttgccat
    20221 acgagccacc gccggaccaa aacggaaact ctgtgtcagc cacaaccggt ccgcattctt
    20281 cagtgcagga tgggaaagcg cattattcgc accccgaaag cggtaaatct gttgataaca
    20341 atctccaaca aggaccaccc gacacttctg ccccagaacc agagcagtgg tcaccggact
    20401 cgcatcctgc gcctcatcaa acaggatggt gtcccatcgg tgagacaggt ctgcaccgga
    20461 caactggaag agtttcagat aagtatcgtg cgtcaccggg aacgtccccc cggggttgct
    20521 catttcgttc cagagcaact gcaccgcccg aagcgcatct gctggcggaa caccccgcat
    20581 ctcatcctta tccggcaggt gatgcaggcc cggtaccgca tcggcagaac agataaagtg
    20641 attcagcccc gtccctgcca cccgtgccag agcccagtat cgtgtattca gctttctcgc
    20701 cacatcagta atacgtaatc ccggaaccag acgatgacgg tagtgtcttc caacccgggc
    20761 atacgccagc tgatgtgacg ttctgcattc cacgttaaag ggaaatttac gctctgcctc
    20821 atcacgtatt gcccggttat aggcaagata gagcatacgg ctgtccggac tggcccgggc
    20881 aaactgtacc agcgtactcg tcttccctgt gccggaaaac gcattcacaa ccagttgctg
    20941 cccctgccag ttaataatgg cgtactgttc atctgtggga ttaaaagaca tgtattctct
    21001 tctccggaaa aacatttccg taatctgatg catcaggggt atgcccacca caaataactc
    21061 tgtacagcaa cagaaaaatt tccctggtca tatgatgaat taatcatcgc catcacaccg
    21121 gatgcttctc atcctgtcct cagaaaggca ggtggtctcc tgtctgccgc gcttatccag
    21181 gataccggga cctgaaaggt cataatctgg caggaaaaaa taatgtccga agtaaaaaaa
    21241 cacgtttctt attcattaac agttgcagct atgctgtcag ccgccatcgt ctgtcagaca
    21301 gcacatgctg atgtcaacac accgaacatc accggagaaa aaaacaatac aacaacacga
    21361 ttctccggaa atatttacgg cagtgaaaat actgtcacat cagagtcaaa cagtctggtt
    21421 gtgggtaaca gtaattccgt ggagggaggg tataacaaca ttgttattgg taattcctcc
    21481 acggtcaaag cttccggttt gatcagaaaa gtgggcgaag atattgtgct ggatggttac
    21541 agcgtcagcc tcggtaatgc caccagaatt aacggtgatt catctgttgc cataggtctg
    21601 acatcgtccg taaccgggaa cgactccgtg gcactgggca gtcattccag tgctaacgga
    21661 aacaatatcg tatccgtcgg ctctgacaca ctgaaacgcc gcatcacaaa tctggcacag
    21721 gggaaagatg accatgatgc ggtaaatctg atgcagatga ccagtgctgt taaccgggaa
    21781 gctggagaaa gaataaaatc aacaaacaac ctgcagaatc agcttaatac cctctctggt
    21841 caggtcataa ctgaaaccag ggcccgaaca gaaggtgatg cagcagcgct ggctgcagcc
    21901 agagcccaca gcgatgaaaa cgacaaaaga acactggtca gtgcgaatac ctatgctgac
    21961 cagaaagaaa ccgccatcaa taaccgtact gacggattac tgacgaccga acgccaggca
    22021 cgcaccgcgg gcgatactga aactctggct gctgccagaa cgcacagcga tgaaaacgac
    22081 aaaagaacac tggtcagtgc gaatacctat gctgaccaga aagaaaccga catcaataac
    22141 cgtactgacg gattactgac gaccgaacgc caggcacgca ccgcaggcga tactgcaact
    22201 ctggctgctg ccagaacgca cagcgatgaa aacgacaaaa gaacactggt cagtgcgaat
    22261 acttatgctg accagaaaga aaccgacatc aataaccgta ctgacggatt actgacgacc
    22321 gaacgccagg cacgcaccgc aggcgatact gcaactctgg ctgctgccag aacgcacagc
    22381 gatgaaaaca acaaaagaac actgatcagt gcgaatacct atgctgacca gaaagaaatc
    22441 gccatcaata accgtactga cggattactg atgaccgaac gccaggcacg catcgcaggt
    22501 gatgcagaaa ctctggcaac agcagaggca tacagtgacg ccggggattc actgacactg
    22561 aaacaggcaa cagcctacag cgatatcagt acccgccggg cactgtcaga ggccaatcgt
    22621 tataccgaca acaaattcag aaacctgagc aataaaattg aacgggcaga aaaaagactt
    22681 aatgcaggca tcgccggagt gacagcaata tcctccatcc cctatgtggc tgaaaattct
    22741 ttttcatacg ggatcggcgt gggtaactat cagaacgcta atgctgtggc cgcaggcatt
    22801 cagtataaaa catcacgtaa taccaatata cgcctcaaca tctcctggga ctcatctcat
    22861 aacaccgcga ttggtgcagg ggttgccggc ggctggtaaa taatcttgcg tacatacggc
    22921 attatggtat tccgtaatca cattaattca gtacgcaaca cagccccatc cggaacagac
    22981 cactggactg agcgggataa tcaaggaaaa agggcgcgca gtgtggtcag ccttcacaac
    23041 aatccggcgg cagatttcct gccgccatgt tgcccgtaaa catttaagtt cgcaggctaa
    23101 cattattgcc acaagccccg tatttccctg gtgaatcctc aaagcgagtc cctcaccatc
    23161 cagacaaaac acagtatcgt cgattaataa tcactaaaag cgttcgtcgt agcatcatac
    23221 cccctgaatg aacacatccc tgtttgcaag gattctgttg ttctgatatc gacgagtagc
    23281 tgcaactctg tatccgcact tattgcaatc agtgcagtct ggccacgatg cgattcgctg
    23341 acctgagcct tattctccag gcggacttta tgcaatgctc ccctgaaatc ttccattatt
    23401 ttacatgtac aggcatcata ttaaatatgg aaataataac gaacggaaaa gggacacaat
    23461 gaatatcaaa gcaccagtat aaaccaggat gttttgagca atttcttact ctcctgaata
    23521 cacactgtaa atcattagaa actgcactag ccatgctaag tacactctgc tacgaactga
    23581 tgaggaagtg cggtatcaat tacctggaca gcctgatcaa cggagaggta tatctattat
    23641 ctatgtaatt acagggggac tgatatcagc gaaaaagcag gtcagcatat tcactgcccc
    23701 tggtctgaac tgaaacaacg tcagccagaa ataatcctat aatgcctcat ataagtggat
    23761 aacttttcat ccgtcgttga catcatacac ataaaacatt gataacagtc atgaatacac
    23821 cgaattatct ttctgactca gcgtttccag tcagtagcat ttcttccggt aaaggcattt
    23881 ccgaaacgtc acgttcgcca ttaaatacaa aaatactttc tgcgtaatca ttctgtaaca
    23941 aagaattagt taacggcaga ttttttgttt gcataccggt ggcccactga aaaatcccgg
    24001 ggaaacgacg ggcactgatg tgtacagaat tgaggtggta cgcagtaata tctcttgcca
    24061 tcatcataag atggcacatc atatcccgaa cgcaaacctg gcacatgatg aacagacgga
    24121 aggctcagca ggacttctgg ataattatac acgatagcca gttactgctg acctggattt
    24181 attatcatac cacattattt atttcaaaaa ataaattttg aattacttgt ttttacttgt
    24241 atataaatat
//
LOCUS       DAZGAN020000066        24098 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGAN020000066 DAZGAN020000000
VERSION     DAZGAN020000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.066"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..149
                     /locus_tag="ACHKYO_RS14830"
                     /old_locus_tag="pgaptmp_002966"
                     /pseudo
     CDS             1..149
                     /locus_tag="ACHKYO_RS14830"
                     /old_locus_tag="pgaptmp_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005163699.1"
                     /note="catalyzes the formation of glutamate from
                     glutamine; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="ACHKYO_RS14835"
                     /old_locus_tag="pgaptmp_002967"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="ACHKYO_RS14835"
                     /old_locus_tag="pgaptmp_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HGU4429781.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /gene="digH"
                     /locus_tag="ACHKYO_RS14840"
                     /old_locus_tag="pgaptmp_002968"
     CDS             1828..3147
                     /gene="digH"
                     /locus_tag="ACHKYO_RS14840"
                     /old_locus_tag="pgaptmp_002968"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HGU4429782.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGIIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3524..4906
                     /gene="dosC"
                     /locus_tag="ACHKYO_RS14845"
                     /old_locus_tag="pgaptmp_002969"
     CDS             3524..4906
                     /gene="dosC"
                     /locus_tag="ACHKYO_RS14845"
                     /old_locus_tag="pgaptmp_002969"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HGU4429783.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4931..7320
                     /gene="dosP"
                     /locus_tag="ACHKYO_RS14850"
                     /old_locus_tag="pgaptmp_002970"
                     /gene_synonym="pdeO"
                     /pseudo
     CDS             4931..7320
                     /gene="dosP"
                     /locus_tag="ACHKYO_RS14850"
                     /old_locus_tag="pgaptmp_002970"
                     /gene_synonym="pdeO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
     gene            7579..8160
                     /gene="ddpX"
                     /locus_tag="ACHKYO_RS14855"
                     /old_locus_tag="pgaptmp_002971"
     CDS             7579..8160
                     /gene="ddpX"
                     /locus_tag="ACHKYO_RS14855"
                     /old_locus_tag="pgaptmp_002971"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HGU4429784.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIY
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            8174..9724
                     /gene="ddpA"
                     /locus_tag="ACHKYO_RS14860"
                     /old_locus_tag="pgaptmp_002972"
     CDS             8174..9724
                     /gene="ddpA"
                     /locus_tag="ACHKYO_RS14860"
                     /old_locus_tag="pgaptmp_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4429785.1"
                     /translation="MKRSIPFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9726..10748
                     /gene="ddpB"
                     /locus_tag="ACHKYO_RS14865"
                     /old_locus_tag="pgaptmp_002973"
     CDS             9726..10748
                     /gene="ddpB"
                     /locus_tag="ACHKYO_RS14865"
                     /old_locus_tag="pgaptmp_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4429786.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10745..11641
                     /gene="ddpC"
                     /locus_tag="ACHKYO_RS14870"
                     /old_locus_tag="pgaptmp_002974"
     CDS             10745..11641
                     /gene="ddpC"
                     /locus_tag="ACHKYO_RS14870"
                     /old_locus_tag="pgaptmp_002974"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HGU4429787.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11638..12624
                     /gene="ddpD"
                     /locus_tag="ACHKYO_RS14875"
                     /old_locus_tag="pgaptmp_002975"
     CDS             11638..12624
                     /gene="ddpD"
                     /locus_tag="ACHKYO_RS14875"
                     /old_locus_tag="pgaptmp_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4429788.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12617..13543
                     /gene="ddpF"
                     /locus_tag="ACHKYO_RS14880"
                     /old_locus_tag="pgaptmp_002976"
     CDS             12617..13543
                     /gene="ddpF"
                     /locus_tag="ACHKYO_RS14880"
                     /old_locus_tag="pgaptmp_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HGU4429789.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(13599..14030)
                     /gene="osmC"
                     /locus_tag="ACHKYO_RS14885"
                     /old_locus_tag="pgaptmp_002977"
     CDS             complement(13599..14030)
                     /gene="osmC"
                     /locus_tag="ACHKYO_RS14885"
                     /old_locus_tag="pgaptmp_002977"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HGU4429790.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14376..14591
                     /gene="bdm"
                     /locus_tag="ACHKYO_RS14890"
                     /old_locus_tag="pgaptmp_002978"
     CDS             14376..14591
                     /gene="bdm"
                     /locus_tag="ACHKYO_RS14890"
                     /old_locus_tag="pgaptmp_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HGU4429791.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            14693..14830
                     /gene="sra"
                     /locus_tag="ACHKYO_RS14895"
                     /old_locus_tag="pgaptmp_002979"
     CDS             14693..14830
                     /gene="sra"
                     /locus_tag="ACHKYO_RS14895"
                     /old_locus_tag="pgaptmp_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HGU4429792.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            14987..16684
                     /gene="maeA"
                     /locus_tag="ACHKYO_RS14900"
                     /old_locus_tag="pgaptmp_002980"
     CDS             14987..16684
                     /gene="maeA"
                     /locus_tag="ACHKYO_RS14900"
                     /old_locus_tag="pgaptmp_002980"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU4429793.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            16818..17828
                     /gene="adhP"
                     /locus_tag="ACHKYO_RS14905"
                     /old_locus_tag="pgaptmp_002981"
     CDS             16818..17828
                     /gene="adhP"
                     /locus_tag="ACHKYO_RS14905"
                     /old_locus_tag="pgaptmp_002981"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGU4429794.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            17975..18259
                     /gene="yddM"
                     /locus_tag="ACHKYO_RS14910"
                     /old_locus_tag="pgaptmp_002982"
     CDS             17975..18259
                     /gene="yddM"
                     /locus_tag="ACHKYO_RS14910"
                     /old_locus_tag="pgaptmp_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4429795.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(18310..18963)
                     /gene="fdnI"
                     /locus_tag="ACHKYO_RS14915"
                     /old_locus_tag="pgaptmp_002983"
     CDS             complement(18310..18963)
                     /gene="fdnI"
                     /locus_tag="ACHKYO_RS14915"
                     /old_locus_tag="pgaptmp_002983"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HGU4429796.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(18956..19840)
                     /gene="fdnH"
                     /locus_tag="ACHKYO_RS14920"
                     /old_locus_tag="pgaptmp_002984"
     CDS             complement(18956..19840)
                     /gene="fdnH"
                     /locus_tag="ACHKYO_RS14920"
                     /old_locus_tag="pgaptmp_002984"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HGU4429797.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(19853..22900)
                     /gene="fdnG"
                     /locus_tag="ACHKYO_RS14925"
                     /old_locus_tag="pgaptmp_002985"
     CDS             complement(19853..22900)
                     /gene="fdnG"
                     /locus_tag="ACHKYO_RS14925"
                     /old_locus_tag="pgaptmp_002985"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22313..22315),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU4429798.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23132..24013
                     /gene="yddG"
                     /locus_tag="ACHKYO_RS14930"
                     /old_locus_tag="pgaptmp_002986"
     CDS             23132..24013
                     /gene="yddG"
                     /locus_tag="ACHKYO_RS14930"
                     /old_locus_tag="pgaptmp_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HGU4429799.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5902 a         6106 c         6097 g         5993 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg acaatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttatctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg tgtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgtgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaattg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taagtcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact agttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcggctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtattatca gggaattgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcgtgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtccta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgccgttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgctatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtagg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggaatttg aataacagaa gtttgcgggt
     4321 gaagtttcta ttacagataa gaaataccgt atcgcaaatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctctcaggcc ttttatgaca acgtccgcag
     4621 tagtgattat gttttccgct acgggggcga tgaatttatc attgttttga ctgaagcttc
     4681 tgaaaacgaa acgttacgta ccgcagaacg tattcgcagt cgggtggaga aaaccaaact
     4741 gaaagccgca aacggcgagg atattgccct ctcactttcc atcggtgccg ccatgtttaa
     4801 tggtcatcct gactatgagc gcctcattca aatagccgat gaagctctgt atatcgccaa
     4861 aagacgaggt agaaaccgtg ttgaactctg gaaagccagt ctttagatgc gccaggatgc
     4921 agaggtaatc atgaagctaa ccgatgcgga taatgccgcc gatggcattt ttttccccgc
     4981 ccttgagcaa aatatgatgg gtgcggtgtt aattaacgaa aataatgaag tgatgttttt
     5041 caaccccgcc gcagagaagc tctgggggta caaacgtgaa gaagtcattg gcaataacat
     5101 tgatatgctg attccgcggg atttgcgtcc tgcgcatcct gaatacattc gtcataaccg
     5161 tgaaggcggt aaagcgcgtg tcgaggggat gagtcgggag ctgcagctgg agaaaaaaga
     5221 cggcagtaaa atctggaccc gttttgcgct atcgaaagtg agcgccgagg ggaaagttta
     5281 ttacctggcg ctggtacggg atgccagcgt agaaatggcg caaaaagaac agactcgaca
     5341 attgattatt gccgttgacc atctcgaccg accggtgatt gtcctcgatc cggaacgcca
     5401 tattgtgcag tgcaatcgcg catttaccga aatgtttggt tactgcatta gcgaagccag
     5461 cggtatgcag cccgatacac tcctgaacat tcctgaattc cctgccgata accgcattcg
     5521 tttacaacag ttgctatgga aaaccgcccg cgatcaggat gaatttctgc tgttgacgcg
     5581 caccggtgaa aaaatctgga ttaaagcctc tatcagcccg gtttatgacg tgctcgcgca
     5641 tctgcagaac ctggtaatga ccttctcgga tatcaccgaa gaacggcaaa tccgccagct
     5701 tgaaggcaat attctcgccg ccatgtgcag cagcccgcca tttcatgaaa tgggggaaat
     5761 catttgtcgt aacatcgaat ctgttctcaa cgaatcgcat gtttcgctgt tcgcactgcg
     5821 caacgggatg ccgatacact gggcgtcatc ttcgcacggt gcagaagttc aaaatgcgca
     5881 aagctggtca gcgaccattc gtcagcgtga tggcgcgcct gcggggatcc tgcaaattaa
     5941 aacctcgtca ggagcagaaa ccagcgcctt tatcgaacgc gtggcagata tcagtcagca
     6001 tatggccgcg ctggcgctgg aacaggaaaa aagccgccag catattgaac aactcatcca
     6061 atttgatccg atgaccggtc tgccaaatcg caataacctg cacaattacc tcgatgacct
     6121 ggtcgacaaa gccgtctctc cggtggtgta tctcatcggt gttgaccata ttcaggatgt
     6181 gattgatagc cttggctatg cgtgggccga tcaggcattg ctggaagtgg tcaatcgctt
     6241 tcgtgaaaaa ctcaaaccgg atcagtatct ctgtcgtatc gaaggtacgc agtttgtcct
     6301 cgtgagcctg gaaaacgacg tcagtaacat tacccaaatc gccgatgagc tacggaatgt
     6361 ggtcagcaag ccgataatga ttgacgataa acccttcccg cttaccttga gtattggcat
     6421 cagctacgac gtgggtaaaa accgcgatta cctgctctcc actgctcaca atgcaatgga
     6481 ttatattcgc aagaatggcg gtaacggctg gcagttcttc agcccggcga tgaacgaaat
     6541 ggtaaaagag cgtttggttt taggcgcagc gctgaaagaa gcgattagca ataaccaact
     6601 gaaacttgtt taccagccgc aaatcttcgc agaaacgggt gaactgtacg gcatcgaagc
     6661 ccttgcgcgc tggcacgatc cccagcatgg tcatgtgccc ccttcacggt ttattcctct
     6721 cgcagaagag attggtgaaa tcgaaaatat tgggcgctgg gtcatcgcgg aagcttgccg
     6781 tcagttagca gaatggcgta gccagaatat tcatatcccg gcgctttccg ttaacttgtc
     6841 ggcactgcac tttcgcagta atcaactgcc taatcaggtg tctgatgcaa tgcacgcctg
     6901 gggtattgac ggccggtaga aatcacggaa agcatgatga tggaacacga taccgaaatc
     6961 tttaagcgca ttcagatcct gcgtgatatg ggcgtgggct tatcggtaga tgattttggt
     7021 acgggctttt ccggattatc ccgcttagtc agtcttccgg taacggaaat caaaattgac
     7081 aaaagttttg tcgatcgttg tctgaccgaa aaacgcatcc ttgccttact tgaagccatt
     7141 accagcattg ggcaaagcct caatttaacc gtcgtggcgg aaggcgtcga aaccaaagag
     7201 caatttgaga tgctacgcaa gatccactgt cgcgttattc agggatattt cttttcccgc
     7261 cccctacccg ccgaagaaat tccaggctgg atgagcagcg tgttaccgct gaaaatctga
     7321 caaattcctc tcgcccgcac tcgcgggttt tcatttaacg tgacactgtc acttaaatac
     7381 tgtgatttca gctacgattc cgggagattc cttccttaac ttcccctctt gcaccaaaac
     7441 tgcactacgc tgcacaaatg ttgatcaaaa gttaaaaaaa tataacttta agccagcatt
     7501 ctggcgctta tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt
     7561 cttatacagg aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtaatcttc
     7621 cctgatctgg agatcgaatt gaaatacgcc tgcgctgata acatcacagg taaagctatt
     7681 tatcagcaag cgcgttgtct gctacacaag gatgcgatta ccgcgctggc gaaaagtatc
     7741 agtatcgccc agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa
     7801 gcacaggcga tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc
     7861 ggttctaatc acagccgtgg cacggcgatc gacctgacgc ttcgtgatga gcacgggaac
     7921 atcctcgata tgggcgcagg gttcgatgaa atgcacgagc gctcccatgc ctatcaccct
     7981 tccgtcccgc ccgccgctca gcgcaatcgg ctgttgctga atgcgataat gactggtggc
     8041 ggttttgtcg gtatctacag cgaatggtgg cacttcgaat taccccaggc agcgagttac
     8101 cctctgctcg ctgatcaatt cacctgtttt atatcccctg gcacacaaca cgtcagttaa
     8161 tcaggaaact gtcatgaaga gatcgatacc gtttcgtccc acattgctcg cgctcgtcct
     8221 tgccacaaat ttcccggttg cgcacgccgc cgtaccgaaa gatatgctgg tgattggtaa
     8281 ggccgccgat ccacaaaccc tcgacccggc ggtaacaata gataataacg actggacggt
     8341 gacctacccg tcttatcagc ggctggttca gtacaaaacg gacggtgata aaggctcaac
     8401 cgacgttgaa ggcgatctgg caagtagctg gaaagcgtct gacgatcaaa aagagtggac
     8461 gttcaccctg aaggacaatg ctaaatttgc cgatggcaca ccggtcactg ccgaagcagt
     8521 aaaactctct tttgagcggc tactaaaaat cggccagggg ccagcagaag catttcccaa
     8581 agatttaaag attgatgctc ccgacgaaca tacggtgaag tttaccctta gccagccatt
     8641 cgcaccgttc ctctacacgc tggcgaatga cggtgcatcc attatcaatc cggcggtctt
     8701 aaaggaacat gcagcggatg atgctcgcgg cttcctcgcg caaaataccg ccggttccgg
     8761 accatttatg ctgaaaagct ggcaaaaagg tcagcaatta gttctggtgc caaatccgca
     8821 ttaccccggc aataaaccga actttaagcg agtatcggta aaaattatcg gtgaaagtgc
     8881 ctcccgtcgc ctgcagctct cccgtggcga cattgacatt gccgatgcgc tgccggtgga
     8941 tcaactcaac gccctgaagc aggaaaataa agtcaatgtg gcagagtatc cgtcactgcg
     9001 cgttacctat ctgtatctga ataacagcaa agcgcctctt aatcaggcgg atctgcgtcg
     9061 ggccatttcc tggtctaccg attatcaggg catggttaac ggcattctga gtggtaacgg
     9121 aaaacagatg cgcggcccga ttccggaagg catgtggggc tacgatgcga cggcaatgca
     9181 atacaaccat gacgaaacga aagccaaagc cgaatgggat aaagtaacga gcaaacccac
     9241 cagcctgacg tttctctact ccgataacga tccgaactgg gagcctattg ctctggcgac
     9301 acaatccagt ctcaacaagc tgggcatcaa tgtgaagctg gaaaagctgg cgaacgccac
     9361 catgcgcgac agagtgggta aaggtgatta cgacattgcg attggcaact ggagtccgga
     9421 ttttgccgac ccgtatatgt ttatgaatta ctggtttgag tcagacaaaa aaggtctgcc
     9481 gggtaaccgc tcgttctatg aaaacagtga ggtcgataag ttactgcgca atgcgcttgc
     9541 gaccaccgac cagacgcagc gtacccggga ctaccagcag gcacagaaaa tcgtcattga
     9601 tgacgctgct tatgtgtacc tgttccagaa aaactaccaa ctggcgatga acaaagaggt
     9661 gaaaggcttt gtgttcaatc ccatgctgga acaggtcttc aatatcaata ccatgagtaa
     9721 ataacgtgac cttctggagt attttacgcc aacgctgctg ggggctggtg ctcgtggtgg
     9781 cgggcgtctg cgtgattacg tttattatct cgcatctgat ccctggcgat ccggcgcggt
     9841 tactggcggg tgaccgcgcc agcgatgcta tcgtggaaaa tattcgccag caactgggac
     9901 tggaccagcc actgtatgta cagttttacc gctacgtcag cgacctgttt catggtgacc
     9961 tgggaacatc cattcgtacc gggcgtccgg tgctggaaga gttgcgtata tttttcccgg
    10021 cgacgctgga gctggctttt ggtgccctgc tgctggcact cctgattggc atcccgctgg
    10081 gcatactctc tgcagtctgg cgaaatcgct ggctggatca tctggtgcga ataatggcca
    10141 ttaccggaat ctccacacct gcgttctggc ttgggctggg cgtcattgtg ctgttttatg
    10201 gtcatctgca aattcttccc ggcggcggaa ggcttgatga ctggctggat ccaccaacgc
    10261 acgttaccgg cttttatctg ctcgatgcgc tgcttgaagg caacggtgaa gtcttcttca
    10321 atgcgttgca acatctcatc ttaccggcat taacgctggc gttcgttcac ctgggaattg
    10381 tcgctcgcca gatccgctca gcgatgctgg aacaattgag tgaagactac attcgtaccg
    10441 cccgggccag cggcttgccc ggctggtata tcgttttatg ttatgcgcta cccaatgcgt
    10501 tgatcccatc gattaccgta ttgggtttgg cgctgggcga tttgttgtat ggcgcagtgc
    10561 tcaccgaaac cgtttttgcc tggcccggaa tgggtgcatg ggtagtaaca tcaatacagg
    10621 cgctcgactt cccggcagtg atgggctttg ccgtcgtggt ttcatttgct tatgtgctgg
    10681 tcaacctggt ggtggatttg ctctatttgt ggattgatcc gcgtatcgga cgtggaggtg
    10741 gtgaatgatg ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa
    10801 cggtgcaaaa ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat
    10861 cattgtatta atgctattga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa
    10921 cgccattgat ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga
    10981 tgaagtggga cgcgatctgt tcagccgcgt actggtcggc agtcagcaat caattctcgc
    11041 cggattagtc gtggttgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg
    11101 tgtgcttggc ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat
    11161 tccttcgttg gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc
    11221 catgctggcg attgctattg tgcgaattcc cttttatgtg cgcctggcgc gggggcaagc
    11281 attagttgta cgccagtata cttatgttca ggcggcgaaa acctttggcg cgtctcgttg
    11341 gcatctgatc aactggcata ttttacgtaa ctccctgccg ccgctgatcg tgcaggcatc
    11401 gctggatatc ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc
    11461 acaacaaccg agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga
    11521 tcagtggtgg tattgcgcat ttccgggggc agcgattttg cttaccgccg tcgggtttaa
    11581 tctctttggt gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg
    11641 acccaacccg ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat
    11701 gttcacgcgc tcaacaatgt gtccttgcag attaaccgcg gtgaaattgt cggtctggtg
    11761 ggagaatccg gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctacagacg
    11821 ggcagttatt gcgtacatcg ggggcagatt tcactgctgg gagacgatgt tcttaacgct
    11881 cgggaaaaac agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg
    11941 atgaccgccc tcaatccgac acttcgaata ggtcttcaga tgatggacgt gatccgccat
    12001 catcaaccaa taagtcgtcg ggaagccaga gctaaagcga ttgccctgct ggaagagatg
    12061 caaatcccgg atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg
    12121 cgccagcggg taatgattgc gctggcattc tcctgcgagc cgcaattgat tattgccgac
    12181 gaaccgacta cggcgctgga cgtcacggtg cagttgcagg tactgcgttt gcttaaacat
    12241 aaagcccgcg ccagtggaac tgcggttctg ttcatcagcc atgatatggc cgtggtgtcg
    12301 caactgtgcg atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgtgacg
    12361 gcagatgtta tccatcatcc ccggcatccg tataccattg gtttgctcca atgcgcacca
    12421 gaacatggag taccacgcca gctattaccc gccattccag ggacggtacc aaacctcacc
    12481 catttgcctg aaggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgaa
    12541 aacgtcccgg cgctgacagc gtgtggtgac aacaaccagc gctgcgcctg ttggtatccg
    12601 cagcaggagg tcataagtgt ctgacacgtt attaacgtta cgcgacgtcc atatcaattt
    12661 cccggcccgt aaaaactggc ttggtaaaac tacggaacat gttcatgcca ttaatggtat
    12721 tgatttacag atccgccgtg gtgaaacctt agggatcgtc ggcgagtcag gctgcggcaa
    12781 aagcaccctc gcacagcttt taatgggcat gctgcaaccg agccacgggc agtacatccg
    12841 ttctggctca caacgcatta tgcagatggt gtttcaggac ccgctctctt cgctcaatcc
    12901 gcgcttaccg gtgtggcgca tcatcacaga accgctctgg atagctaagc gtagtagtga
    12961 acaacagcgg cgagcgttgg cagaggagct ggctgtgcag gtgggtattc gtccggagta
    13021 tctcgaccgc ctgcctcatg cgttctccgg cgggcagcgg caacgcatcg ccattgccag
    13081 agcactctct tcgcagcctg acgtgattgt gcttgatgag ccaacctcgg cgctggatat
    13141 ctccgtgcag gcgcagatcc tcaatttact ggtaacgcta caggaaaatc acgggctgac
    13201 ttatgtgctg atttcacaca atgtctcggt gatacgtcat atgagcgatc gggtggcggt
    13261 gatgtatctc gggcagattg tggaactagg ggaagcgcaa caggtgttga ctgctccggc
    13321 acatccatat acccgattat tgctggattc cctccccgcc attgataaac cgctggagga
    13381 agaatgggca ttacgtaaaa cggatctgcc gggaaaccgc acgttgccgc aaggctgttt
    13441 tttctacgaa cgttgccctc tggcaaccca cggatgtgaa gtccggcaat cattaacaat
    13501 aagggaggac ggacgtgaga tccggtgctg gcgggcgctg tagagatgct aacaacgcat
    13561 caggcattca cgcgcctgac gcgtcatccg gcaatgcttt acgatttcaa ctggtaatca
    13621 agcgtaattt ccgctttcag tacctgagaa accgggcatc ctgcttttgc tttctggatg
    13681 atgccgtcaa aggtagaggc atcaataccc ggcaccgcaa cttcactctt cagtgcgatt
    13741 ttcgtaatcg caaaaccggc atccacttta tccagcgaca catcggcggt ggtatcaatc
    13801 gatgttggcg taaatcccgc ttcccccagc attaatgaaa gcgccattga gaaacatgcg
    13861 gcatgcgctg cgccaatcag ttcttcaggg ttagttcctt tttcgccttc aaaacgcgtg
    13921 ttaaatccat acggctgttg gttcagcaca ccactctcgg tggatactgt tcccttcccg
    13981 cgtttgatat cgccttccca gtgtgcctga cctttcttat ggattgtcat tgttgctctc
    14041 ctgtgggctt aaaaaggaaa ttatagacta cgtgagaaac agcacgagga taagcaggat
    14101 attccgaata aaaatccggc agaaatatta cttctgcact taaattaaat ttccacaata
    14161 gataaccggg aatatataac ccaagagata ttgcattaat attaatcaac tcaccacaat
    14221 aataatacct atcaatatca tgaggttaaa atataaaaat catcaaccag gactaatctt
    14281 aacaacgaag cggcaaatat ttgccatgct gaatgtgctt tataaatctg cgatccgtag
    14341 cagacaccat aaatacacag acacggagaa tcactatgtt tacttattat caggcagaaa
    14401 attcaacagc agaacccgct ctggttaatg ccattgagca aggccttcgg gcagagcacg
    14461 gtgttgtcac tgaagatgac attctcatgg agctgaccaa atgggtggaa gcttctgata
    14521 acgacatcct cagtgatatc taccagcaaa cgatcaatta tgtggtcagt ggccagcacc
    14581 ctacgcttta aggtgctatg cttgatcggc aacctaattt aggggtttag cacgtgtttc
    14641 ttcgctacgg cgatgttgtc cttaaaacta gcaacaggat tgaggagtta aaatgaaatc
    14701 gaaccgtcag gcacgtcata ttcttggact ggaccataaa atttctaacc agcgcaaaat
    14761 agttaccgaa ggtgacaaat ccagcgtggt aaataatcca accggcagaa aacgccccgc
    14821 tgaaaagtaa ttcataacca tcagtcctca atgacgatta aacaccattg cctgcgcaat
    14881 ggtgtttttg tttttatctg ctctatactt ggggccgacg ccctggcggt aaagcaaaga
    14941 cgataaaagc gtgccaggga tggatattca aaaaagagtg agtgacatgg aaccaaaaac
    15001 aaaaaaacag cgttcgcttt atatccctta cgctggccct gtactgctgg aatttccgtt
    15061 gttgaataaa ggcagtgcct tcagcatgga agaacgccgt aacttcaacc tgctggggtt
    15121 actgccggaa gtggtcgaaa ccatcgaaga acaagcggaa cgagcatgga tccagtatca
    15181 gggattcaaa accgaaatcg acaaacacat ctacctgcgt aacatccagg acaccaacga
    15241 aaccctcttc taccgtctgg taaacaatca tcttgatgag atgatgcctg ttatttatac
    15301 cccaaccgtc ggcgcagcct gtgagcgttt ttctgagatc taccgccgtt cacgcggcgt
    15361 gtttatctct taccagaacc ggcacaatat ggacgatatt ctgcaaaacg tgccgaacca
    15421 taatattaaa gtgattgtgg tgactgacgg tgaacgtatt ctggggcttg gtgaccaggg
    15481 catcggcggg atgggcattc cgatcggtaa actgtcgctc tataccgcct gtggcggcat
    15541 cagcccggcg tatacccttc cggtggtgct ggatgtcgga acgaacaacc aacagctgct
    15601 taacgatccg ctgtatatgg gctggcgtaa tccacgtatc actgacgatg aatactatga
    15661 attcgttgat gaatttatcc aggctgtgaa acaacgctgg ccggacgtgc tgttgcagtt
    15721 tgaagacttt gctcaaaaaa atgcgatgcc gttacttaac cgctatcgca atgaaatttg
    15781 ttcttttaac gatgacattc agggcactgc ggcagtaaca gtcggcacac tgatcgcagc
    15841 cagccgcgcg gcaggtggtc agttaagcga gaaaaaaatc gtcttccttg gcgcaggttc
    15901 agcgggatgc ggcattgccg aaatgatcat ctcccagacc cagcgcgaag gattaagcga
    15961 ggaagcggcg cggcagaaag tctttatggt cgatcgcttt ggcctgctga cggacaagat
    16021 gccgaacctg ctacctttcc agaccaaact ggtgcagaag cgcgaaaacc tcagtgactg
    16081 ggataccgac agcgatgtgt tgtcactgct ggatgtggtg cgcaatgtaa aaccagatat
    16141 tctgattggc gtctcaggac agaccgggct gtttacggaa gagatcattc gtgagatgca
    16201 taaacactgt ccgcgtccga tcgtgatgcc gctgtctaac ccgacgtcac gcgtggaagc
    16261 cacaccgcag gacattatcg cctggaccga aggtaacgcg ctggtcgcca ctggcagccc
    16321 gtttaatcca gtggtatgga aagataaaat ctaccctatc gcccagtgta acaacgcctt
    16381 tattttccca ggcatcgggc tgggtgttat tgcttccggc gcgtcacgta tcaccgatga
    16441 gatgctgatg tcagcaagtg aaacgcttgc tcagtattcg ccgctggtgc tgaacggcga
    16501 aggtctggta ctaccggaac tgaaagatat tcagaaagtc tcccgcgcaa ttgcgtttgc
    16561 ggttggcaaa atggcgcagc agcaaggcgt ggcggtgaaa acgtctgccg aagctctgca
    16621 acaagccatt gacgataatt tctggcaagc cgaataccgc gactaccgcc gtacctccat
    16681 ctaagcctgc gcccggtagt gaaggctacc gggctatttc cctctccctt tttcagatct
    16741 catccatact gggtagtggc gaataaatct catttgcctc acctgctatg cagaacatca
    16801 tccgaaaagg aggaactatg aaggctgcag ttgttacgaa ggatcatcat gttgacgtta
    16861 cggataaaac actgcgctca ctgaaacatg gcgaagccct gctgaaaatg gagtgttgtg
    16921 gtgtatgtca taccgatctt catgttaaga atggcgattt tggtgacaaa actggggtaa
    16981 ttctgggcca tgaaggcatc ggtgtggtgg cagaagtggg tccaggtgtc acctcattaa
    17041 aaccaggcga tcgtgccagc gtggcgtggt tctacgaagg atgcggtcat tgcgaatact
    17101 gtaacagtgg taacgaaacg ctctgccgtt cagttaaaaa tgccggatac agcgttgatg
    17161 gcgggatggc ggaagagtgc atcgtggtcg ccgattacgc ggtaaaagtg ccagatggtc
    17221 tggactcggc ggcggccagc agcattacct gtgcgggggt caccacctac aaagccgtta
    17281 aactgtcaaa aattcgtccc ggtcagtgga ttgctatcta cggtcttggc ggtctgggta
    17341 acctcgccct gcaatacgcg aagaatgtct ttaacgccaa agtgatcgcc attgatgtca
    17401 atgatgagca gttaaaactg gcaaccgaaa tgggtgcaga tttagcgatt aactcacgca
    17461 ccgaagacgc cgccaaaatt gtgcaggaga aaaccggtgg tgcacacgct gcggtggtga
    17521 cagcggtagc taaagctgcg tttaactcgg cagttgatgc tgtccgtgca ggcggtcgtg
    17581 ttgtggctgt cggtctgccg ccggagtcta tgagcctgga tatcccacgc cttgtgctgg
    17641 atggcattga agtggtcggt tcgctggtcg gcacgcgcca ggatctaacc gaagccttcc
    17701 agtttgccgc cgaaggcaaa gtggtgccga aagtcgccct gcgtccgtta gcggacatca
    17761 acaccatctt taccgagatg gaagaaggca aaatccgtgg ccgtatggtg attgatttcc
    17821 gccgttaaga ggcctttgct gcgagtgcca gtcctggtgt cgcagcatcg cactctctcc
    17881 gacattaaat ggctaaatgg attattgttc cttcctacgc aacaataatg atagtgttcc
    17941 tgatgaagga acactactga gagtaaagac actgatgaaa atggccaatc atccccgccc
    18001 gggggacatt attcaggaat cactggacga acttaatgtc agcctgcgcg agtttgccag
    18061 agcaatggaa attgcgccct caacggcaag ccgattgctg accggaaaag cagctttgac
    18121 accagaaatg gcgattaaac tttccgtggt gatcggcagt tcgccgcaaa tgtggctgaa
    18181 tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg gtggatgtgt cgcgactacg
    18241 ccgtctggtt acgcaataaa aaaatggcgc ttcttaaagc gccattttca gcttaaagtt
    18301 caaaagagat tatatccctt cttcactctc ttttttcgct tctgccttct cgatttcacg
    18361 ataccagcgc ggatggtgtt tcttcgccca gcgacggctc accttccctt cgatcatccc
    18421 tttaatcgac cctttcaccc agaatgccat atacatatgg atcaggatgg cgtggatcag
    18481 gatgataccc gcagccgcgt ggatcagcag gctgtagcga acaacctgca tcgggaagta
    18541 ctgcgcaaag tacggacgcc agataatcac cccggtcacc agcagcacga aaatcatgct
    18601 catgatcgac cagaacatca tcttttgccc ggcgttgtac ttaccgacat ccgccacttt
    18661 atgctcattg cctttcaata cttcgacaat gttcaacagc cacggaatat ctttcttatc
    18721 cgggatgttg tgatgcacaa aacgcacaaa cataaacatc agtgcgacga aaatcgcaat
    18781 gccgaaaaac gggtgcaaaa tgcgtcccat ctgcggcgta ccgaaggttt gcgtcagcca
    18841 ttgcagcgtc gggaagaaga acgaaatccc ggacagcgcc accaggaaga agcaaatcac
    18901 cacggtccag tgacaggcgc gatcaataaa tttggtgcgc acaatcattt tcgacttact
    18961 catgatgatc ctcctcgtca tcgtccactt ccttattcgg gccaataccg atgtagtgga
    19021 aaatcaaccc ggcaaaagtg gcaataaagc cagccgctgc cagcggtttc aacgcgcctt
    19081 tccacaggct taccgaggtg tcgatcttcg gatctttcgg cagaccgtga tacagctccg
    19141 gctgatcggc gtgatgcagc acgtacataa cgtgcgtacc accgacccct tccgggttgt
    19201 agacgccagc atgttcgtaa ccacgcgctt tcagtttcgc cacgcgctgt tccgccagct
    19261 ccagcatctc cttcttggtg ccgaagtgga tagccccggt cggacaggtt ttcacacaag
    19321 ccggttcctg gccgacgctg acgcgatcga cgcagagcgt gcatttatat acccggttat
    19381 cctctttgtt gaggcgcgga atattaaacg gacacccggc aatgcagtaa ccacagccga
    19441 tgcagttttc cgactggaaa tcgacaatcc cgttagcgta ctgaatgatt gcaccagcag
    19501 acgggcacgc cttcaggcag ccgggatctt cacagtgcat acagccgtct ttacggatca
    19561 gccactccag cttgccgttc tgttcggttt cgctaaagcg catcaccgtc caggacttgg
    19621 cgctcagatc ggcggggtta tcgtaaaccc cgacgcagtg ccccacttca tcacggatgt
    19681 cgttccactc cgaacacgcc acctgacagg ctttacagcc gatacaggtg gaaacgtcga
    19741 taagttttgc gacttctgct ttgtaatcac gcacctgaga aggcggcgtg atggagttag
    19801 ttgcggacct tttgataatg tcctgcgttt ccatagccat ctgttcgccc ccttacgcct
    19861 tctcgatgtt gactaagaac gctttatatt ccggcgtttg cgagtttgca tcaccgacat
    19921 tcggcgtcag agtgttagcg atataacctt tacgcgcgac accctcaaag ccccagtgga
    19981 ttggaatacc caccgtttca acctgctgac catttacatt cagtggtttc agacgacgcg
    20041 ttaccacagc caccgcgcgg ataaagccac gcttgcttga gacagtgaca cgatcgccat
    20101 tattaatgcc tttcgccgcc gccagcgttt cgctgatttc cacaaactgt tccggctgag
    20161 caattgcgtt gagcaatgcg tgcttggtcc aggtgtggaa gtgctcggtc agacgatagg
    20221 tcgtacccac atacgggaac tgctcttttt tacccatccg cagtgcgtct tgttcataca
    20281 gacgaacaac cgggttagac accacgttcg gatgcagcgg gttagtgccc agcggcgttt
    20341 caatcggctc gtagtgttcc gggaacggac cttccgccat tttgttgatg gcaaacaggc
    20401 gtcccatccc ttccggctgc atgataaacg gcccggttgg cgtacccggt gcggcattgc
    20461 cgaagtcagg aatatcgtta cccgtccact tgctgccgtt ccactggatc agcatccgtt
    20521 tcggatccca cggtttaccg ttgatatccg ccgaagcacg gttgtacagc acgcgacggt
    20581 tgagcggcca cgcccaggcc catcccagcg tattccccag accggacggg tcggagttat
    20641 cgcggttagc catctggttg ccctgctctg tccagctacc ggtgtagatc cagcaagaag
    20701 atgcggttgt accgtcatca cgcagatgcg caaagctact cagcaactga cctttcttcg
    20761 caatcagcac gccattagca tcatagagat cttccagcgc atagccgttg ttctctttag
    20821 ctacttcgtc agattgcggt tcgtgcggct gcttgtagtt ccagctcatc ttcatcagcg
    20881 gttctacgcc tttaccacct tcggcctggt acagctcgcg caggtgatgg tagatacccg
    20941 ccagaatttc accgtcgtta cgcgcttcgc ccggcgcatc ctgacctttc cagtgccact
    21001 gcagccagcg accggagtta gcaatagaac catcttcttc agcaaagcag gtcgaaggca
    21061 gacggaatac ttcagtctga atagacgccg gatcgacatc gttcgactcg ccgtggttct
    21121 gccagaaggt tgaggtttca gtcaccagcg gatcgataac caccatgtac ttcagcttgc
    21181 tcaggcagct caccactttg tttttgtccg ggaaggacgc aaccgggtta aagccctggc
    21241 agaaataacc ggtgactttg ccttcatcca tcatgttgaa atacttgatg acgtcgtagg
    21301 tctggtccca cttcggcagc cagtcatagc cccagttgtt ctctttctgc gcggcatcgc
    21361 catagaaaga tttcatcagg ctaacgaaga acttcggata gttgctccag tagttcacct
    21421 gaccagccag cgtcgctttc ggcgtgttcg cttccagata cgactgcaaa tcaacctgtt
    21481 tttctgacgg cagcgtcaga taacctggca ggctggtaga gagcagacct aagtcagtca
    21541 gaccctgaat gttggagtga ccacgcaatg cgttcacgcc gccaccggcc atacccatgt
    21601 taccgagcag caactggatc atcgccatag tacggatgtt ctgcgcaccc acggtgtgct
    21661 gcgtccagcc cagcgcgtac aggaaggtgg ttgtgcgatc cggtgcgctg gtggaggcca
    21721 gcacttcaca cactttcagg aagtcggctt ttggcgtacc acagatgttt tcaacaacgt
    21781 ccggcgtgta gcgggaaacg tgcgctttca gcaggttcca cacacagcgc ggatgagtca
    21841 gtgtttcatc gcgtttcgca tagccgtttt catcgaactg atagttccag gacgatttat
    21901 cgtattgccg tttttcagcg tcgtagccgc tgaacagacc gtcttcgaaa gcaaaatcat
    21961 cacgcaccag caggctggcg ttggtgtaat gtttaacgta ttcggcgttg attttgttgt
    22021 tttcgatcag gtagcgcaaa acgccagaca ggaacgtaat gtccgtaccg gaacgaatag
    22081 gtgcgtaaat atccgccaca gaagcggtac gcgtaaaacg gggatcgaca acgatcaagg
    22141 ttgcatcgtt gttgtttttc gcttccatcg cccagcggaa accgacggga tgcgcttcag
    22201 cagcgttacc gcccattacc attacgacgt tagcgttttt gatatccacc cagtggttgg
    22261 tcatcgcacc gcgaccaaat gttggagcaa gacttgctac cgttggtccg tgtcagacgc
    22321 gcgcctggtt gtctaccgcc agcatcccga gggagcgggc aaatttctgt gtcagcatcc
    22381 cggtttcgtt gctggcaccg gaggcacaca gcataccggt agaaagccaa cggtttaccg
    22441 ttacgccctg ctcgttcttt tcaataaagt tagcgtcacg gtcagctttc atcagctttg
    22501 caatacggga gaatgcttct tcccagctaa tgcgctgcca tttgtcagaa cctggcgcac
    22561 gatattccgg gtagcgcaga cggttttcgc tgttgacgta atccagcaaa ccggcccctt
    22621 ttgggcacag cgcaccacgg cttaccggat gatccgggtc accttcaatg tgataaatcg
    22681 cttctctggc gtttttcgcg ccatcaccca ggctatacat caatagcccg caacctacgg
    22741 aacagtatgt gcaggtgtta cggatctctt tagcgcgtaa taatttgtag tttcgcgcct
    22801 gagccagtgc ttgcttcggg gcaaagccca atgccgctac cgttgttcca gccataccgc
    22861 ccgcgcagat tttaaaaaat tgtctgcgac tgacgtccat tgctttcctc ttttttcagg
    22921 ggtattactg cgcgaggaaa ctaacagtaa aattctgaat ctttttgcgc gaaatcaaaa
    22981 agaacacgtg tttgccacaa aataatccgc ctgcgcaaat tgacctacct caatagcggt
    23041 agaaaaacgc accactgcct gacaggccag ttaaaaaaat gctataaaat tcagcttaat
    23101 ttttaacggc aagagagaca aaacagcgag catgacacga caaaaagcaa cgctcatagg
    23161 gctaatagcg atcgtcctgt ggagcacgat ggtaggattg attcgcggtg tcagcgaggg
    23221 gctcggcccg gtcggcggcg cagctgctat ctattcatta agcgggctgc tgttaatctt
    23281 cacggttgga tttccgcgta ttcggcagat tccgaaaggc tatttactcg ccgggagtct
    23341 gttattcgtc agctatgaaa tctgtctggc gctttcctta gggtatgcgg cgacccgtca
    23401 tcaggcgatt gaagtgggta tggtgaacta tctatggccc agcctgacaa ttctctttgc
    23461 cattctgttt aatggtcaga aaaccaactg gctgattgta ccaggattat tattagccct
    23521 ggtcggcgtc tgctgggtgt taggcggtga taatgggtta cattatgatg aaatcatcaa
    23581 taatattacc accagcccat tgagttattt cctggcgttt attggtgcgt ttatctgggc
    23641 agcctattgc acagtaacga ataaatacgc acgcggattt aacggaatta ccgtttttgt
    23701 cctgctaacg ggagcaagtc tgtgggttta ctattttctc acgccacaac cagaaatggt
    23761 atttagcacg cccgtcatga ttaaactcat ctctgcggca tttaccttag gatttgctta
    23821 tgctgcatgg aatgtcggta tactgcatgg caatgtcacc ataatggcgg taggctcgta
    23881 ttttacgccc gtactttcct cagcgcttgc agccgtgctg ctcagcgccc cgctgtcgtt
    23941 ctcattctgg caaggcgcgc taatggtctg cggcggttcc ctgctctgct ggctggcgac
    24001 acgtcgtggt taaaataatt gccgggtcta cggcccggca gttaatcaga taaatttcga
    24061 caatattaaa aaataaattt caaataccag taattcac
//
LOCUS       DAZGAN020000067        23731 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGAN020000067 DAZGAN020000000
VERSION     DAZGAN020000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.067"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            414..695
                     /gene="drpB"
                     /locus_tag="ACHKYO_RS14935"
                     /old_locus_tag="pgaptmp_002987"
     CDS             414..695
                     /gene="drpB"
                     /locus_tag="ACHKYO_RS14935"
                     /old_locus_tag="pgaptmp_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HGU4429800.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            735..1430
                     /gene="yedJ"
                     /locus_tag="ACHKYO_RS14940"
                     /old_locus_tag="pgaptmp_002988"
     CDS             735..1430
                     /gene="yedJ"
                     /locus_tag="ACHKYO_RS14940"
                     /old_locus_tag="pgaptmp_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HGU4429801.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSSAG"
     gene            1497..2915
                     /gene="dcm"
                     /locus_tag="ACHKYO_RS14945"
                     /old_locus_tag="pgaptmp_002989"
     CDS             1497..2915
                     /gene="dcm"
                     /locus_tag="ACHKYO_RS14945"
                     /old_locus_tag="pgaptmp_002989"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HGU4429802.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVVDAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2896..3366
                     /gene="vsr"
                     /locus_tag="ACHKYO_RS14950"
                     /old_locus_tag="pgaptmp_002990"
     CDS             2896..3366
                     /gene="vsr"
                     /locus_tag="ACHKYO_RS14950"
                     /old_locus_tag="pgaptmp_002990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HGU4429803.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3355..4275)
                     /gene="yedA"
                     /locus_tag="ACHKYO_RS14955"
                     /old_locus_tag="pgaptmp_002991"
     CDS             complement(3355..4275)
                     /gene="yedA"
                     /locus_tag="ACHKYO_RS14955"
                     /old_locus_tag="pgaptmp_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HGU4429804.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4448..5365
                     /gene="yedI"
                     /locus_tag="ACHKYO_RS14960"
                     /old_locus_tag="pgaptmp_002992"
     CDS             4448..5365
                     /gene="yedI"
                     /locus_tag="ACHKYO_RS14960"
                     /old_locus_tag="pgaptmp_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HGU4429805.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKVKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            5444..5626
                     /gene="yodC"
                     /locus_tag="ACHKYO_RS14965"
                     /old_locus_tag="pgaptmp_002993"
     CDS             5444..5626
                     /gene="yodC"
                     /locus_tag="ACHKYO_RS14965"
                     /old_locus_tag="pgaptmp_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HGU4429806.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPAEEV"
     gene            5864..7558
                     /gene="dgcQ"
                     /locus_tag="ACHKYO_RS14970"
                     /old_locus_tag="pgaptmp_002994"
     CDS             5864..7558
                     /gene="dgcQ"
                     /locus_tag="ACHKYO_RS14970"
                     /old_locus_tag="pgaptmp_002994"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HGU4429807.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLYQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(7555..8370)
                     /gene="yedP"
                     /locus_tag="ACHKYO_RS14975"
                     /old_locus_tag="pgaptmp_002995"
     CDS             complement(7555..8370)
                     /gene="yedP"
                     /locus_tag="ACHKYO_RS14975"
                     /old_locus_tag="pgaptmp_002995"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HGU4429808.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWGEGLDHFFSAR"
     gene            complement(8668..8895)
                     /gene="yodD"
                     /locus_tag="ACHKYO_RS14980"
                     /old_locus_tag="pgaptmp_002996"
     CDS             complement(8668..8895)
                     /gene="yodD"
                     /locus_tag="ACHKYO_RS14980"
                     /old_locus_tag="pgaptmp_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HGU4429809.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9058..9246
                     /gene="dsrB"
                     /locus_tag="ACHKYO_RS14985"
                     /old_locus_tag="pgaptmp_002997"
     CDS             9058..9246
                     /gene="dsrB"
                     /locus_tag="ACHKYO_RS14985"
                     /old_locus_tag="pgaptmp_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HGU4429810.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9290..9913)
                     /gene="rcsA"
                     /locus_tag="ACHKYO_RS14990"
                     /old_locus_tag="pgaptmp_002998"
     CDS             complement(9290..9913)
                     /gene="rcsA"
                     /locus_tag="ACHKYO_RS14990"
                     /old_locus_tag="pgaptmp_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HGU4429811.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10203..10988)
                     /gene="fliR"
                     /locus_tag="ACHKYO_RS14995"
                     /old_locus_tag="pgaptmp_002999"
     CDS             complement(10203..10988)
                     /gene="fliR"
                     /locus_tag="ACHKYO_RS14995"
                     /old_locus_tag="pgaptmp_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGU4429812.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(10997..11266)
                     /gene="fliQ"
                     /locus_tag="ACHKYO_RS15000"
                     /old_locus_tag="pgaptmp_003000"
     CDS             complement(10997..11266)
                     /gene="fliQ"
                     /locus_tag="ACHKYO_RS15000"
                     /old_locus_tag="pgaptmp_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HGU4429813.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11276..12013)
                     /gene="fliP"
                     /locus_tag="ACHKYO_RS15005"
                     /old_locus_tag="pgaptmp_003001"
     CDS             complement(11276..12013)
                     /gene="fliP"
                     /locus_tag="ACHKYO_RS15005"
                     /old_locus_tag="pgaptmp_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGU4429814.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMLPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12013..12378)
                     /gene="fliO"
                     /locus_tag="ACHKYO_RS15010"
                     /old_locus_tag="pgaptmp_003002"
     CDS             complement(12013..12378)
                     /gene="fliO"
                     /locus_tag="ACHKYO_RS15010"
                     /old_locus_tag="pgaptmp_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HGU4429815.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12381..12794)
                     /gene="fliN"
                     /locus_tag="ACHKYO_RS15015"
                     /old_locus_tag="pgaptmp_003003"
     CDS             complement(12381..12794)
                     /gene="fliN"
                     /locus_tag="ACHKYO_RS15015"
                     /old_locus_tag="pgaptmp_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HGU4429816.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(12791..13795)
                     /gene="fliM"
                     /locus_tag="ACHKYO_RS15020"
                     /old_locus_tag="pgaptmp_003004"
     CDS             complement(12791..13795)
                     /gene="fliM"
                     /locus_tag="ACHKYO_RS15020"
                     /old_locus_tag="pgaptmp_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HGU4429817.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVHQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(13800..14264)
                     /gene="fliL"
                     /locus_tag="ACHKYO_RS15025"
                     /old_locus_tag="pgaptmp_003005"
     CDS             complement(13800..14264)
                     /gene="fliL"
                     /locus_tag="ACHKYO_RS15025"
                     /old_locus_tag="pgaptmp_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HGU4429818.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGVTLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14369..15496)
                     /gene="fliK"
                     /locus_tag="ACHKYO_RS15030"
                     /old_locus_tag="pgaptmp_003006"
     CDS             complement(14369..15496)
                     /gene="fliK"
                     /locus_tag="ACHKYO_RS15030"
                     /old_locus_tag="pgaptmp_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGU4429819.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSIQGRVTGNSGVDIFA"
     gene            complement(15493..15936)
                     /gene="fliJ"
                     /locus_tag="ACHKYO_RS15035"
                     /old_locus_tag="pgaptmp_003007"
     CDS             complement(15493..15936)
                     /gene="fliJ"
                     /locus_tag="ACHKYO_RS15035"
                     /old_locus_tag="pgaptmp_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HGU4429820.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(15955..17328)
                     /gene="fliI"
                     /locus_tag="ACHKYO_RS15040"
                     /old_locus_tag="pgaptmp_003008"
     CDS             complement(15955..17328)
                     /gene="fliI"
                     /locus_tag="ACHKYO_RS15040"
                     /old_locus_tag="pgaptmp_003008"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGU4429821.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(17328..18014)
                     /gene="fliH"
                     /locus_tag="ACHKYO_RS15045"
                     /old_locus_tag="pgaptmp_003009"
     CDS             complement(17328..18014)
                     /gene="fliH"
                     /locus_tag="ACHKYO_RS15045"
                     /old_locus_tag="pgaptmp_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HGU4429822.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18007..19002)
                     /gene="fliG"
                     /locus_tag="ACHKYO_RS15050"
                     /old_locus_tag="pgaptmp_003010"
     CDS             complement(18007..19002)
                     /gene="fliG"
                     /locus_tag="ACHKYO_RS15050"
                     /old_locus_tag="pgaptmp_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGU4429823.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVYDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(18995..20653)
                     /gene="fliF"
                     /locus_tag="ACHKYO_RS15055"
                     /old_locus_tag="pgaptmp_003011"
     CDS             complement(18995..20653)
                     /gene="fliF"
                     /locus_tag="ACHKYO_RS15055"
                     /old_locus_tag="pgaptmp_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HGU4429824.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            20868..21182
                     /gene="fliE"
                     /locus_tag="ACHKYO_RS15060"
                     /old_locus_tag="pgaptmp_003012"
     CDS             20868..21182
                     /gene="fliE"
                     /locus_tag="ACHKYO_RS15060"
                     /old_locus_tag="pgaptmp_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGU4429825.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            21516..21848
                     /gene="emrE"
                     /locus_tag="ACHKYO_RS15065"
                     /old_locus_tag="pgaptmp_003013"
     CDS             21516..21848
                     /gene="emrE"
                     /locus_tag="ACHKYO_RS15065"
                     /old_locus_tag="pgaptmp_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HGU4429826.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            22017..22568
                     /gene="ybcL"
                     /locus_tag="ACHKYO_RS15070"
                     /old_locus_tag="pgaptmp_003014"
     CDS             22017..22568
                     /gene="ybcL"
                     /locus_tag="ACHKYO_RS15070"
                     /old_locus_tag="pgaptmp_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HGU4429827.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            22578..23375
                     /gene="ybcM"
                     /locus_tag="ACHKYO_RS15075"
                     /old_locus_tag="pgaptmp_003015"
     CDS             22578..23375
                     /gene="ybcM"
                     /locus_tag="ACHKYO_RS15075"
                     /old_locus_tag="pgaptmp_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4429828.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT         5762 a         5935 c         6175 g         5859 t
ORIGIN      
        1 agcggaaaca tagggggtat caatgattgt tgcgccaaac gactggtgtg atcaggcgca
       61 cattaatgaa ggcataaaaa aaagccggca tcatgccggc ccaaaaatat aaacaagacg
      121 ataaaaggct aataacggag gcatcatgac acagaaaaat aaaaaggtat aattgtattc
      181 agtgctaata tattgattaa atgaattaat aaagccgatc tcaaagataa ataaaatcgc
      241 tcaaaacgat ttttcctgta ttcactgccg ttgcgcaaaa attatctatt tgttcaaaaa
      301 atgagtgagt cttgactggc tcatccaatg tggaaaaatg tgacttttat cacataatag
      361 tactaagtct gaattttccg ggttatctca aaatggaata cggttcgaca aagatggaag
      421 agagactctc tcgcagccct ggcggcaaac tggctttgtg ggctttctat acatggtgtg
      481 gctatttcgt ctgggcaatg gcgcgttaca tctgggtgat gagccggatc cccgatgccc
      541 ccgtgtcggg atttgaaagc gatttaggct ctaccgcggg aaagtggtta ggggcactgg
      601 tcggattttt atttatggct ctggtcgggg cattgttagg cagcattgcc tggtacaccc
      661 gacctcgtcc cgcacgtagc agacgctatg aataaaaact gctaaggtgg cgactctttg
      721 gcaacggagc gcgtatggac ttacaacact ggcaggcaca gttcgaaaac tggttgaaaa
      781 accatcatca gcaccaggac gcggcccatg acgtgtgtca ttttcgccgc gtctgggcta
      841 cggcacagaa gctggcggca gatgacgatg tcgatatgct ggtgatttta accgcctgtt
      901 attttcacga tattgtgagc ctggcgaaaa atcatccgca aaggcagcgt tcttcaatcc
      961 tggcggcaga agagacgcgt cgcctgctcc gtgaagagtt cgagcaattt ccggcggaga
     1021 aaatcgaggc cgtttgtcat gccattgccg ctcacagttt cagcgcgcaa atcgccccct
     1081 taacaacgga agctaaaatc gtccaggatg cggatcggct ggaagccctg ggggcgattg
     1141 gcctggcgcg cgtgtttgcc gtttcagggg cattgggcgt ggcgctgttt gatggtgaag
     1201 atccgttcgc acagcatcgc ccgcttgatg ataaacgcta cgcgctggat catttccaga
     1261 ctaagttgct gaagctaccg caaaccatgc aaaccgcaag aggcaagcag ctggcgcagc
     1321 acaacgcgca ttttttagtc gagtttatgg cgaagctcag tgccgaactg gcgggggaga
     1381 atgaaggtgt cgatcacaag gtgatagatg cgttttcatc cgctggctga gcgcgtggcc
     1441 ctaaatggct gtaattatgt taacctgtcg gccatctcag atggccggtg aaatctatgc
     1501 aggaaaatat atcagtaacc gattcataca gcaccgggaa tgccgcacag gcaatgctgg
     1561 agaaactgct gcaaatttat gatgttaaaa cgctggtggc gcagcttaat ggtgtaggtg
     1621 agaatcactg gagcgcggca attttaaaac gtgcgctggc gaatgactcg gcatggcacc
     1681 gtttaagtga gaaagagttc gcccatctgc aaacgttatt acccaaacca ccggcacatc
     1741 atccgcatta tgcgtttcgc tttatcgatc tattcgccgg aattggcggc atccgtcgcg
     1801 gttttgaatc gattggcgga cagtgcgtgt ttaccagcga atggaacaaa catgcggtac
     1861 gcacttataa agccaaccat tattgcgatc cggcgacgca tcattttaat gaagatatcc
     1921 gcgacatcac cctcagccat aaagaaggcg tgagtgatga ggcggcggcg gaacatattc
     1981 gtcaacacat tcctgaacac gatgttttac tggccggttt cccttgtcag ccattttcgc
     2041 tggctggcgt atcgaaaaag aactcgctcg ggcgggcgca cggttttgcc tgcgatactc
     2101 agggcacgct gttctttgat gtggtgcgca ttatcgacgc gcgtcgtccg gcgatgtttg
     2161 tgctcgaaaa cgtcaaaaac ctgaaaagtc acgaccaggg taaaacgttc cgcatcatca
     2221 tgcagacgct ggacgaactg ggctatgacg tggttgatgc agaagataac gggccagacg
     2281 atccgaaaat catcgacggc aaacattttc tgccgcagca ccgtgaacgc atcgtgctgg
     2341 tgggttttcg tcgcgatctg aatctgaaag ccgattttac cctgcgtgat atcagcgaat
     2401 gtttccctgc gcagcgagtg acgctggcgc agctgctgga cccgatggtc gaggcgaaat
     2461 atatcctgac gccggtgctg tggaagtacc tctatcgata tgcgaaaaaa catcaggcgc
     2521 gcggtaacgg cttcggttat ggaatggttt atccgaacaa tccgcaaagc gtcacgcgta
     2581 cgctgtctgc gcgttattac aaagatggcg cggaaatttt aatcgatcgc ggctgggata
     2641 tggccaaagg tgagaaagac tttgacgatc cgcagaatca gcaacatcgt ccacgtcggt
     2701 taacgcctcg tgaatgcgcg cgcttaatgg gttttgaagc gccgggagaa gcgaaattcc
     2761 gcattccggt ttcggacact caggcctatc gccagttcgg taactcggtg gtcgtgccag
     2821 tctttgccgc ggtggcaaaa ctgcttgagc caaaaatcaa acaggcggtg gcgttgcgtc
     2881 agcaagaggc acaacatggc cgacgttcac gataaggcca ctcgcagcaa aaatatgcgc
     2941 gcgattgcca cgcgtgatac ggcgatagag aagcgcctcg ccagtctgtt aaccgggcag
     3001 ggcctggcgt ttcgcgttca ggacgccagt ctgcccggac gcccggattt tgtcgttgat
     3061 gaatatcgct gcgtgatatt tacccacggc tgcttctggc atcatcatca ctgctatctg
     3121 tttaaagtgc ctgcgactcg aaccgagttc tggctggaga agataggtaa aaatgttgag
     3181 cgcgatcgcc gcgatatcag tcgcttgcag gaactcggct ggcgcgtatt gattgtctgg
     3241 gagtgcgcgt tacgtgggcg cgagaagctg acggatgaag cgcttagcga gcgtctggaa
     3301 gagtggatct gcggcgaagg tgccagcgcg cagatcgaca cgcaggggat tcatttactc
     3361 gcttgatgcg tcctgaataa ctggcgcaac tacgggtttt gccggaaaga gatattttcc
     3421 caacgtgacc agcaccaccg cgaagacaat tacgccgagc gccagccatt caatcttcga
     3481 cagcgtctct ccgcccagtc ccgtacccag caagaccgcg accaccgggt taacgtaggc
     3541 gtagctggtg gcgagagccg gactgacatt acggattaaa tacatataag cgttgatggc
     3601 gataatcgaa ccaaacagcg ccagatagcc gaccgcaagg aagcctgaaa gggaagggag
     3661 cgccgtcagt ttttcacctg caatcatcga cgcgatcatt aacaccacgc ctgccgccag
     3721 catctcaata gcacccgcca tcatccctac aggtaaggta atgcgcgagc cataaaccga
     3781 gccaaacgcc cagctaatcg agccgattaa aatcagaatc gcgccccacg gattgccgct
     3841 taaatttcca ccgctattga gcatgatgat tccggcaagc ccaatggcga tacccaccca
     3901 ttccagtttg cgcgttttaa tgccaaacag gcggctgaag cacagggtaa agaggggcac
     3961 ggttgcaacc acaacggcgg cgatgccgga aggaacattt tgatgctcgg caaccgtcac
     4021 catgccatta ccgacagcca gcaataacag gccaatcagc gcggcattga gcagcggacg
     4081 tagcgggggg agcttgtgtc cgcgcagtag caaaaatgcc atcaataaaa taccggctgc
     4141 caggaatcga acgcctgcca tcattaacgg aggccagctt tccacgccaa tccgaatgac
     4201 aaaataggtt gagccccaaa tgatatacaa cgcaaacagc gcgccaaaaa gcggtaacaa
     4261 ctggcggaaa cgcataatcc ctcacggtgg aaataaaaag gtggttcata gtaaacgtga
     4321 aaatcattct gctggcgaga gatataattg cacttaattg ttaaaaaaat gttgacctgt
     4381 gaagcaagta tcagagctgc cgtttttgct tcatacttac acccttcaac aataaaaatg
     4441 agagggaatg cttttggccg ggagtagttt actgacgttg ctcgatgata tcgccacact
     4501 actggacgat atctccgtga tgggcaaact ggcggcgaag aaaaccgccg gtgtattagg
     4561 ggatgactta tcgctcaatg cgcaacaagt ttcaggcgtg cgggccaacc gggaacttcc
     4621 cgtggtctgg ggcgtggcga aaggatcgct gattaataaa gtgattctgg tgccgctggc
     4681 gctgatcatc agtgcgttta tcccgtgggc gattacgcct ctgttgatga ttggtggcgc
     4741 gtttctctgc tttgaaggag tagagaaagt gctgcatatg ctggaggcgc gtaaacataa
     4801 agaagatccg gcgcagagcc agcagcgtct ggagaagctg gcggcgcagg atccgctgaa
     4861 gtttgaaaag gacaaagtaa aaggggcgat tcgtaccgat tttatattgt ctgccgaaat
     4921 cgtcgccatc acgctgggga ttgtggccga agcgccgttg cttaatcagg tgctggtgct
     4981 ttcaggcatc gcgctggtag tgaccgtggg cgtctacggt ctggtagggg ttatcgttaa
     5041 gattgatgac ctggggtatt ggctggcgga aaaatccagc gcgctgatgc aggcattagg
     5101 taaagggtta ttgatcattg cgccctggtt gatgaaggcg ttatcgattg tcggcacgct
     5161 ggcgatgttc ctcgtcggcg gcgggattgt ggtacatggt attgcgccgc tgcatcacgc
     5221 cattgaacat ttcgccgggc agcaaagtgc agtggtggcg atgatattac cgactgtttt
     5281 aaatctgatt cttggattta tcatcggcgg catcgtggtg ctgggagtga aagccgtagc
     5341 gaaaatacgc ggtcaggcac attaatactc gcactggcta ttatgcaaaa ttcgccatct
     5401 tcaactaagg tgaacaagtt tcactcgaaa aaaggaggcg agtatgagct ttatggttag
     5461 tgaggaagtt acggttaaag agggcggccc gcggatgatt gtcaccggat actccagcgg
     5521 tatggttgag tgtcgttggt atgacggtta cggggtcaag cgggaagctt ttcatgaaac
     5581 cgagcttgtt ccaggggagg gaagtcgtcc tgcggaagaa gtttgaaagg gtatcattaa
     5641 cacccgaccg gatgtggcgc tcacgtcgca tccggtcggg tgtgccagtg tctgcactga
     5701 attgcctgat gcgacgctat cgcgtcttat catgcctacg ttataaaact atcgatgtct
     5761 gtcgcgtctt atcatgccac cattataaaa ttatcgctgt tttgcggtac gctatcgcca
     5821 ggttatgcca gaatcataaa aaagcaggtt gggagtcgtc agggtgcagc acgagacaaa
     5881 aatggaaaac cagagctggt tgaaaaaact cgcacgccgc ctggggcctg gtcatgtcgt
     5941 taatctctgc tttatcgtgg tattgctttt ttccaccttg ctcacctggc gtgaagtagt
     6001 ggtgctggaa gatgcctata tctccagcca gcgtaatcat ctggaaaacg tagccaacgc
     6061 gctcgataag catttgcagt ataacgtcga caaactgatc tttttgcgta atggcatgcg
     6121 cgaagctctc gtagcgccac tggatttcac ctctctgcgt gatgctgtaa ccgagttcga
     6181 acagcatcgc gacgagcacg cctggaaaat cgaactcaac cgacgacgca ccctgtcagt
     6241 caatggcgta tcggatgcat tagtcagcga ggggaatctc ctgtctcgcg aaaatgaaag
     6301 cctcgacaat gaaattaccg ctgcactgga agttggttac ttgctgcgac tggcgcacaa
     6361 cacctcgtcg atggttgaac aggcgatgta tgtctcgcgt gccggatttt acgtttcgac
     6421 gcagccgacc ttgtttacgc gcaatgtacc aacgcgttat tacggctatg tcacccaacc
     6481 ctggtttatc ggccattcgc aacgagaaaa tcgtcaccgc gcggtacgct ggtttacttc
     6541 gcaaccggaa cacgccagca atactgaacc gcaggttacc gtcagtgttc cagtagacag
     6601 taataactac tggtatggcg tgctggggat gagtattccc gtgcgtacca tgcagcaatt
     6661 tttaagaaac gccatcgata aaaacctcga tggtgagtat cagctctatg acagtaagct
     6721 gagatttttg acctcttcca atcctgacca tccaacaggg aatatttttg atcctcgtga
     6781 actggccttg ctggcgcagg cgatggaaca tgacacgcgg ggcggcattc gtatggacag
     6841 tcgctatgtt agctgggaac gtctggacca tttcgacggt gtgctggtgc gcgtccatac
     6901 gttaagcgaa ggcgtgcgcg gcgatttcgg cagtatcagc attgcattaa ccctgctgtg
     6961 ggcgctcttt accaccatgt tactcatctc ctggtatgtg attcgccgga tggttagcaa
     7021 catgtatgtt ctgcaaagct cgttgcagtg gcaggcgtgg cacgacacct taacgcgttt
     7081 atataaccgt ggcgcactgt tcgaaaaagc ccgtccgctc gctaaattgt atcagacgca
     7141 ccaacatcct ttttctgtca ttcaggtcga tcttgaccat tttaaagcga ttaatgaccg
     7201 ctttggtcat caggcgggcg accgtgttct ttctcatgct gccggattaa ttagcagttc
     7261 cttgcgtgcg caggacgttg ccgggcgggt cggtggtgag gagttttgtg tgattctgcc
     7321 aggcgcgagt ctgacggagg ctgcggaagt cgcagaacgt attcgcctga agttaaatga
     7381 aaaagagatg ttgattgcta agagtacgac gatacgcatc agtgcctcgc tgggggtaag
     7441 tagcagcgag gaaaccggtg attatgattt tgaacaactc cagtcactgg ctgaccgtcg
     7501 actttatctc gctaaacagg ccgggcgtaa tcgggtatgc gcgagcgata acgcttaacg
     7561 ggcggagaaa aaatggtcca gcccttcacc ccagccttcc ggtccttcac gctgcgttcg
     7621 ccagacgcgg gccggatcct catcatgcag atgcacccct tcacggttta gccctttcac
     7681 aatcaccgcg taatccatta cctccagtaa gggcgcatcg tttggcccat cgcccaggcc
     7741 aagtgtggtt gggcgtttgc ctgacaattg ttgataggtc gcgataatcc agttggcagc
     7801 ctgatctttt ccggcagagg catccagtac gtgccagaag cgcgcaccct gcataaactg
     7861 taagcccagt tcgttcagac gagcggtaaa ttgtgccata cgctcgtcac tgtcgcgcca
     7921 gattagcgtt accgacgcct catgcagctg cgtcagcgcc gcctggctac ggcttaatcc
     7981 cgtccattcg gcgatggttg catcgtcgac atcatcgaaa gtcgtgaatt taaaatgttc
     8041 tttctcgcgt agcgtattta aaacctggct gatttcgcca tggctaatac ctgagatgat
     8101 gcgtggaaaa ccgtctatat cctgccattg ttcagcaagc tggatcactg cgccattttc
     8161 ggcaatcagt ggtaaccctt gtagccccaa cgttttttgc aagtacagca tttccgctga
     8221 agttttactg ctacagagaa tgacgggaac attcgcttcg cgtaaacggc tgagccaggg
     8281 ggcagccggt tgccagtcat aactatgact gtccagcagg gtgccatcaa gatcgctaaa
     8341 aaccagtagt ggttgttgaa ttgaaaacat ggtcgcgcag ttctcccctt accaggatga
     8401 ttctcaaaac agcacaaata atcaaagatg attttttcgg gagcgatccc gttgacgcca
     8461 gagccaccaa ggggtgacgc acgtcacatt tctattcata agtagcgtta atcattcata
     8521 tcgcgaatat tttcttgtca gcgaaaaaaa ttgcggataa ggtgatgaac acatcagatt
     8581 tcctggtgta acgaattttt taagtgcttc ttgcttaagc aagtttcatc ccgaccccct
     8641 cagggtcggg atttttttat tgtgcattca acgattcact tcagagacgc tgaagtcatg
     8701 aatatccagt tcgaaggcat ccgccaattc acgccatgta tgatattcac gtccatcgac
     8761 gctaacgtgt tcagaatcgt tctggctgcg atgaacttcg ctttcgctaa tttcattgat
     8821 cgccgccagc agggcatcga catcgacgct gacctcacgt tttgcggtat cgctgtactc
     8881 ttttgcggtt ttcatcatct ttcctcgcaa ccgttactct gttgttgccg ccagtcgtta
     8941 agtccgcctg gcatctctta agtatagacc gtcgagaaaa tcagcactcg cgcgcgcctg
     9001 gcgctgccag aaaaacgaaa ttgttctaca ctggcacaaa gccacaggag gaaaacgatg
     9061 aaggtgaatg atcgggtaac agtcaaaacg gatggcggtc cgcgtcgtcc tggcgtggta
     9121 ctggcagttg aggagtttag tgaaggcaca atgtacctgg tttcgctgga agactacccg
     9181 ctcggcatct ggttctttaa tgaagcaggg catcaggacg gtatctttgt ggagaaagca
     9241 gagtaatctt attcagcctg actggtggga aaccaccagt cagaatgtgt tagcgcatgt
     9301 tgacaaaaat accattagtc acattatccg tcagtcggac gacatggtag ataacctgtt
     9361 tattatgcgt tttgatctta cgtttaatat tacctttatg cgatgaaacg gtcttggctt
     9421 tgatattcat ttggtcagag atttgaatcg ttccctgacc tgccatccac attcgcaaca
     9481 tactcgattc ggttcggctc aatgataacg tcggcatatt taaaaaagag gttatcgttg
     9541 tctctttttt cagaatatcg ccaaggatat cgtcgagaga ttccggttta atcgatttag
     9601 aacttatcaa taaatttttt ctgaccaata gatattcatc aaaatgaaca ttggcaattg
     9661 ccataaaaac gataaataac gtattgggat gttgattaat gatgtgcttg atgtgctgac
     9721 tgttagaagc atcatggatg aaacagtcct cattaataaa caccactgaa gggcgctgtg
     9781 aatcacaagc tatggcaagg tcatcaacgg tttcaatgtc gttgatttct ctttttttaa
     9841 cccctctact caacagatac ccggttaaac ctagtcgggt gtaactacat aaatccataa
     9901 taatcgttga catggcatac cctcactcaa tgcgtaacga taattcccct tacctgaata
     9961 tttcatcatg actaaacgga acaacatggg tcacctaatg cgccactctc gcgatttttc
    10021 aggcggactt actatcccgt aaagtgttgt ataatttgcc tggaattgtc ttaaagtaaa
    10081 gtaaatgttg cgatatgtga gtgagcttaa aacaaatatt tcgccacagg agtatcctgg
    10141 aagatgttcg caggtattat ttttcggata atccttagga taacatgata aacgttacgg
    10201 aattatatta atggcaattc actaataata tcagccagca aattaaacat ctcactgaat
    10261 aaatgttcgc aaaaaggtgc aattaacggc attaatgccg ccattaaaga gataccgaca
    10321 gtcagagtta atggaaatcc aataacaaaa atggataatt gcggggccat acgattaagt
    10381 aaacctaatg ccagattcag tgtcagcagc agagtaatga gcggtaacgc cagcatcagc
    10441 ccgttaagga aaatcaaact ccctgctttg gtgagtgcca gaaacgcatt gctgttcaac
    10501 ggttcgccac caattggcag ggtgtgaaag gtatcgacca gcagtgaaat caaccataaa
    10561 tgaccgttaa atgtcaggaa cagtagtaac gccagcatat ccatgatacg cgctaaaaca
    10621 ggcatattga gatggctggc cggatcgaca aacgtcgcaa atgacagccc catttgcaga
    10681 ccgataattt cgccagcggt tcgcacagcg gcaaaggcaa attgcatggt aaaaccaagc
    10741 gcaatgccga tcaggatctg ctgcacggcc agccacagag caaagaacga aaaaacagga
    10801 acatcgttgg caggtaatga tggggcaata gcgaacgtga tcatcattgc cagacccagt
    10861 tttacccgtt tcggcacgct gcgttcgctc agaattggcg cggtagaaat gagcgccagc
    10921 acgcgcagta acggccagaa gtacagactt agccaggaaa gccattgatc gcttgtcacc
    10981 tgcatcattg tacgggctac ccgatgatat acggcaggtt agtgaacaag gtgcggacgt
    11041 aatccagcaa cagattgagc atccacggtc cggcaataat aatggcaata aatacggcga
    11101 tgatttttgg aataaacgac agcgtcattt cgttgatctg ggtggcggcc tgcaaaatac
    11161 tgatgataag gcccgtgacc agcgccacca gcaacagcgg ggcagccagt gccagcgcga
    11221 ctttcatcgc ttcagtcccc atcatcatga ccgattcagg tgtcattttg cctctctagc
    11281 tgtaaaagct ctgcgccagc gaaccgacca gcaattgcca gccatccacc agtacaaaca
    11341 gcatcagttt aaagggcaga gcaatggtgg cgggggggag catcatcatc cccaatgcca
    11401 tcaacacgct ggcaatcacc aggtcgataa tcaaaaaagg gatgaaaatc gtgaagccta
    11461 tctgaaatgc ggttttcaac tcgctggtca cgtaggccgg gagcaaaatg cgcatcggca
    11521 cggcttcagg tccctgcaac gggccggtat tcgctagtct ggcaaacaac cctaaatctg
    11581 cctcacgggt ctgacgcagc ataaactcac gtagcggctg cgcccctttt tccagcgcct
    11641 cctgcattga tattttctct tcgctgaatg gctggtacgc atcgacataa attttgtcga
    11701 tcaccggtga cataataaaa aaggtcaaaa atagtgccag ccccagcaat acctggttag
    11761 gtggcgcgga gggcgttccc agcgcgttac gcaataaacc aaaaacaatg atgatgcggg
    11821 tgaaactggt catcatcagt aaaattgccg gaataaacgt caacgaggtg atgaacacca
    11881 gcgtctgcac cgggagcgac cagctttgtc caccgccagg cagcggctgg ctggtgatac
    11941 ccggcagttg cgcgaaggcg aggggcgtaa ccagccagag aaggacaggt gcgacagaca
    12001 acaaacgacg catcaggatc tcccgctacg cttaagcaaa tttttcatga ccgactgaaa
    12061 atcggtctgc ggtatctctt ctgttggtgc tgaaggggga agtttatgca gcagattgat
    12121 ttgccctgcg gtaacgccga gcaccagccg tgcatcttcc acatcgacca ccacaacccg
    12181 ttcacgcgcg cccagcgaag cactggcgct aattttcaga ccgttaacgc cagtgcgttt
    12241 cggggcaaat cccaaccgtt ttaccagcca ggcagcagcg aggatcaggg caataatggc
    12301 gatgagtgcg ccgctgacct gcagcagtgg cgcagcagaa accggcgcgg aagattgcac
    12361 agtagcgtgg ttattcatca ctaacggctc aggcgacgca ttcgctcaga cggagtaatg
    12421 atatcggtga tccgcacgcc atatttatcg gcaacgacca ccacttcgcc ctgggcgatt
    12481 aaataaccgt tgatcagaat atccagtggt tcgcccgcca gaccgtccag cgccacgacg
    12541 gacccttgcg tcagacgcaa cagctctttg atggtcatcc gcgtacggcc cagctcgacg
    12601 gtcagcttga ccggaatatc cattatcagg tcgatatcct gcaacgttcc gctgacatca
    12661 ccaccgccaa attgctggaa caccgcgtcg gcagcgcttt tgctgctggt tgatttttgt
    12721 tcgctcaacg cttcagccca cagatcgtcc attgcgccgt tgttgtcatc ggccggatta
    12781 ttcatgtcac tcatttgggc tgttcctcgt tcagagaatt taaaatcggg ttaatcaaat
    12841 gttctatccg taacgcatac tgaccgttga gggtgccgta ctgactggtc agtaccggaa
    12901 cgccgtcaac atgagcgatg atgcgatcgg gtttttctat cggcaggacg tcgcctggtt
    12961 tcagttttaa aatctgcgac aggcgtagcg agatatcggc aaagttggcg accaactcca
    13021 gctgtgaatg ctgcacctgg tgcaccaggt tatcgcgcca gttctgatct tcattacgcg
    13081 agttttccag cggcgggtta accaacaatt cccgtagcgg ctcaatcatg ctgaatggca
    13141 gacagatatt aaattcgccg gtcaggttgc caatctccac atggaacggc gtgttaacca
    13201 caatgtcgtt cggcgaggtg gtgatattgg taaatttcac ctgcatttcc gaacgcacgt
    13261 actcaacttc cagcggatta atcgccttcc aggcgtcgct atagccttca agcgccagct
    13321 ttaacatgcg gttgatgacg cgctgttcgg tatgggtaaa ctcgcgacct tccactttgg
    13381 tcgggaagcg tccatcgccg ccaaacaggt tatccacggc gataaacacc agactcggtg
    13441 aaaacaccac cagcccagtg ccgcgcagcg gtttcagatg gataaggttc aggttggtcg
    13501 gcaccggcag gttgcgggca aattcatggt acggctgaat gcggatggcc ccgacggtta
    13561 tatccgggct acgacgcagc aggttgaaca gccccatacg aaaatggcgg gcaaagcgct
    13621 cattaatgat ttccagcgcc tgcaaacgtt cgcgcacaac ccgtcgttgg gtattcggat
    13681 cgtacggacg aatgtcactt tcgccgctaa cactggctgt cggttcgtct ttgacttcgc
    13741 tgtcaccatt caacagcgca tcaatttcag cttgagaaag aatactatcg cccatgtcgt
    13801 tatcgcagaa taaaagcggt atacagcacg tcggtgacat cctgtttcgg ttgcccggca
    13861 acaagcgggg tggaaagcgt ggttttaatc tcggcaatca ggtttttctt gccttcttct
    13921 gtcgccagta cggcagcatc ctgacgcgaa aacagtaaca gcaaacggct acggacttcc
    13981 ggcaaatact cactcagccg cgagcgggta gcttcatctt tcaggcgcag ggttacgccg
    14041 atataaagca cgcgatccgc atcgcccaaa ttgaccgtga aggtatccag cgcgtagaag
    14101 accggggagg gtacgacgcg ttgctgcgct ttgtcgtcag cggcaacctg atgcgaatgc
    14161 cagtagctgt aacctgcgct ggcacaggcc gcgagggtaa tgaataccag aatcgggatc
    14221 caaagcgatc gcttgctttt cttgcttatc gcgtaatcag tcatgtgttg cggtcttcct
    14281 gtgtcgctac tgcttatcgg ctaattatcc tgcgtcttga gcgcaacaaa gcggggaaaa
    14341 gacgcggatt acggtgctac ctctgacgtt aggcgaaaat atcaacgccg ctgttgcctg
    14401 ttacgcgccc ttgtatagag acgggaaccg gaagcgtatc gtcgtcttcc cccgccagag
    14461 gttcatggtt tactgtgcgt tggctttgct gttgttggga agcggcctgc tgctgaccac
    14521 taaagctttc gccactgatg ttgctttgcc ctaactgaat gccactttcg gccagctgcg
    14581 tacgcagtac cggaagcgct gcttccagcg cggcgcgtac atgctgatgc ggtgaaacca
    14641 tctggatttg tgcctggtta tcatccactt tgagggagat ttgcacttca cctaaatcct
    14701 gcgggtgcag acgcaactct gcactttgtt gcccctggcg ggtgaatagc gaaatatgct
    14761 ggcttaatga ttgttgccat tcgtgagaac ccagcggtgc actcaaaaca ggcgcggcga
    14821 cggtgggcag tggctgtgtc tggtgtggag taattagcgg gctggcggca gcggtcaccg
    14881 gtgacggtgt gctgatgact tccgctttac tctgggcttc tgctaccagc ggtgttaatg
    14941 gctgagctgg cgtgccgggg gcatcatcag gctgtgctgt tgtgagttgc gcagaagtca
    15001 gttttgtgaa gagcgttggt ttctctgccg gtaacacggt tgacggcaca tcagtcactt
    15061 tgggcgtatt gtcaaaaccc ggcaacatcg caaaaagggc gctcaggctt gcggtgacgt
    15121 cttcattcag atcatccgct ttttcgtctt ttgtcgtatt tttgtcagcc accgcagcca
    15181 acgtcatcgt ctgagcggtg gttaacggtg ttgatgtgga ttgttcgacg ttgatgacag
    15241 gcagtgtttc atccacaggg atcagtaaat ccgcttgttg cgcgtcggaa acaatatcgc
    15301 tgatcagcag ctcgcctttt gtcgtgggct tatctgttgc caccagcaac tggggggccg
    15361 ctttgtcggt agttgtctcg cctgctaatg cttcgctcaa caacgcgaga aaatcttgtg
    15421 cagcatcgct ggctttgccg ccaggcaatg tggtggtgtc aacgtcggcg gtaattaagg
    15481 gcgctaagcg aatcattcag gtttcctcat ggcggcgcgc tgggcgaact catccatctt
    15541 tttctgatcg aggcggtttt ctgcaagcag tgccgccgtg gattgccgtt cctgcagtgt
    15601 ctgccaggcc tgcaaacgtt gtttcttttc tcgccagctg ttcagggcaa tgtcaacttt
    15661 ctgcgtccac tgattaagtt gctggcgatg ctgagtaatg gctttttcca gcgtctggat
    15721 aaactgctga tagttgatcc agcggttgct ggtcatcccg gcactcatat cgctgttgag
    15781 gttattgcga tattcattct gataatcgat cagcattttg agctgttctt ccgcctgctg
    15841 acacccgcga cgcatttcac ccagcaggcg cgcggcatcc tctacctctt tttctgccag
    15901 atctttcagg gtcgccagcg cgccatgttc tgccatctgc cgttatctcc tgggttatga
    15961 cactgtcggg aaaatacgct ccagcccctg gagagacgct tcccagtccg cgcgttcaaa
    16021 aatgccttgt tgcaaatagc cctccagctg cggccacagg gcgatggctt tgtcgagcat
    16081 cggatcgctg cctttggcat acgcgccgac gctaaccaga tcgcggttac gctgaaaact
    16141 cgacaacagc tgtttgaagg tgcgcactcg cgcgtaatgt tgctcactga tcaacgccgt
    16201 cattgcgcgg ctgatcgacg cttcaatatc gatagccgga tagtgcccgg cttccgccag
    16261 tcggcgagac agcacaatgt gaccgtcgag gattgcccgc gcagagtcgg caatggggtc
    16321 ctgctggtca tcgccttcag tgagcacggt ataaaatgca gtaatcgatc cgccgccgct
    16381 aatgccattt ccggcacgct cgaccagtgc cggtaatttg gcaaacaccg acgggggata
    16441 acctttagtg gcgggtggtt cgccaatcgc cagcgcaatt tcacgctggg ccatcgcgta
    16501 gcgggtgagg gagtccataa tcagcaacac atgctgacca cgatcgcgaa aatcttcggc
    16561 aatgcgcgtg gcataggcgg caccctgcat tcgcaggaga ggagaaacat ccgccggagc
    16621 ggcaatcacc acggagcgtg cacgcccttc ggcaccgagg atattctcaa taaaatcttt
    16681 tacttcgcgt ccgcgttcac caatcaaacc cacgacaatg acatcggcgc gggtgtaacg
    16741 tgccatcatc cccagcagca cacttttacc aacgccggac ccggcaaaca gtcccatacg
    16801 ctgcccacgc ccaacggtaa gcagggcatt gattgggcgc acgccggtgt ccagcacatg
    16861 ttcaatcggt gtacgttgca acgggttaaa tggcggggta atcagcgcac cggtttccgt
    16921 cgtatcgggg gagggcaggc catcgagcgg cttaccgctg ccgtccagaa cgcgacctaa
    16981 taacgccgga ccgagcggca actgcttgcc gctttgcagt ccttcagccg aaatgttttt
    17041 ggcataaacc cgcgcgccgg gcaggacacc ttcgacttcc tccagcggca ttaaaaacag
    17101 ccgttgaccg ttaaagccaa cgacttcgct ttctacttcg tgcgtttcgc tgccgttctg
    17161 gcgttcaatg acacaggttg cgccgagcgg caattgtaat ccggtggctt ccagcaccag
    17221 cccggtagcg cgggttaatc gcccatagcg acgtaccgca ggcaactgcg ccattttggc
    17281 ttcaaagtta tccagcgtgg ttagccagcg agtcaggcgc gtggtcatta caccactcct
    17341 ggtgctgcca gacggcagag ttcttgccag cgagtggcga cactggcgtc gagatcgcct
    17401 tcatcggcgg agactttaca gccgccagga tggagtgtgg gatcgccccg caagcgccag
    17461 ccatgcaaac ttaaggttgc gccgagcata tcatccacac gttgcagatc atccgggtgc
    17521 acgcgcagct gtggtttacc gctgaataac ggttcttgct gcaacaactg ttggatctgt
    17581 ttgatcagtg ccgagttatc cactgttggc gtctgaccga tgacctgacg tgccgcctcc
    17641 agcgccatct gcatcaggcg cgaagcaatc acactatcaa gcgcatcaag ggtagtttga
    17701 aattcgctga ccagttgctg catccgggca tgaattggcg cttgttgaga cttcgcctct
    17761 gccagacctt gctccagccc ccgggccagt ccttcctgat agccctgctc atgaccttgc
    17821 tggcgacctt cggcaatccc cgcctgataa ccttgctcat gggcctgcat ttgcagttgc
    17881 gccagttgct gctcaaggct gggttcggcc tcttcaatga tggtttcttc cggctcgacc
    17941 atgggcacaa actctgcctg tggtggcgcg agatcgtccg gcgtccaggt tttccacggc
    18001 agattatcag acataggtat cctcgccgct gccaattacc atctcgccag tttcggcaag
    18061 gcggcgcaca atcagcagaa tcgctttctg ttcgttttcc acctgcgaca gacgcaccgg
    18121 accacggttg gcgagatcgt cgcgcagaat atcggcggca cgctgcgaca tattgcgcag
    18181 gaatttctcg cgcagtggct gctcggcccc tttcagcgcg atcaacagcg attcggaatc
    18241 cacttcctgc aacagacgct gaatgctgcg atcgtagaca tccaccagat tttcgaacag
    18301 gaacatctcg tcgataattt tttgcgccag ctcgccgtcg aattcgcgca cggcggtaat
    18361 aacggcttct tcctgctgag ttttcatcag gttgataatt tcggctgccg ttctcacgcc
    18421 gcccattttg ctgcgcttga gattctgacc gtcgagcaag ccattcagta cttcggtcag
    18481 ttccgccagc gcggctggct gcacgccgcc aaaggtggcg atacgcaaca tcacgtcgtg
    18541 gcgcagacgt tcatcgaaca acgccagaat atcggcggcc tgggcgcgct tcagatgcac
    18601 cagaatggtg gcgataattt gcggatgctc atcgcgaatc agatcggcgg cactctgcgg
    18661 ctccataaag ttgagcgttt caataccgct ggcggtatcg cgagtttcga gaatatcttc
    18721 cagcaggctg gcggcacgtt cctcacccag agctttgacc aataccgaac gcagataatc
    18781 gttggcgttg atattcagtg cggcaaactg ttcagcttct tgctcaaact ccgccagcac
    18841 atcggttagc tgcttgttgg agatctgcgt gacgttcgcc attgcagcgc tcagggtttg
    18901 cacttcgcgc tgggagaggt gcttgaacac ctctgccgcc cggtcttcgc caatggtcat
    18961 cagcaggatg acgcttttat cggtgcctgt caggttactc atgatcgtta tttatccact
    19021 ggcgaatgac cagcgccacc acgcgcggat cgttatcaga catttcacgg atacgctggc
    19081 tcatgacttc tgcccccaga cgttggttag cgcgtcgttg ttggagttgt tcgtctttgc
    19141 tcaggcggac ttccaccgca tcttccactt cctcacgggc ctgcgcctgt tgctgtacag
    19201 ctttcatcgc ctcggcgcga cgtgttaact gcgggcgtac cgctttccgc cacagcagcc
    19261 acgccaccag cagtaccagc aaccagcgac cggcagccag caactgatcg ataaacgctt
    19321 gctgttgcca gaacggcagt tctccgccgc tttcgtcact gctattgaac ggtgagttaa
    19381 cgacattgag cgagtcaccg cgtttttcag aaaagcccat cgcctcgcgg gtcagatctt
    19441 caatttgctt catctgttcg ttgctgagag gcaatggttt accgtctggc aaggttttgt
    19501 aattcaccac taccgcgact gacagacgtt gcacatcgcc cacgttcatt ttggtatgac
    19561 gaatggtgcg atcgacttcg tagttactgg tttcattccg ctgtgtgcta cgcggcccgc
    19621 tattgctggt ggtgctcgcc tgctgctggc ggttattttg atttgccgga ggcgtgctga
    19681 ttggcgcgtt attcgcaggt gccggttgat tcgacaacgc ccccggtacg ccgcccggat
    19741 aaccggaacc gctttgctcg ctctcattaa gctggcgtga acgaagcgcc gcatgagatt
    19801 catcaccgtt agggcgatac tgttcttccg tttgttcttt actggcgaag tccagctgcg
    19861 ccgtgacctg ggcgtgaata ttaccgttac caacaatagg cgacaggatt gcttcaatac
    19921 gccgctgaat acggccttcg acatcgctgg catatttcaa ctgagcatca ttaagatcgc
    19981 gcccgctggt attggactgg gttaacagat gtccgccctg atccaccagc gtgacgtttc
    20041 ccggcggcag accagcaacg gcgctggaaa ccagatgcac aatggcgcta atttgccctt
    20101 catcgagtgc gcggccgggt aacagattta ccgtcaccga tgcagaaggg gatttttgtt
    20161 cacggacgaa taaagacggt ttcggcattg ccagatgtac gcgcgcccct tttaccgggc
    20221 cgatagttgc gatggtacga gaaagctcgc cttccagcgc ccgctgataa ttcacctgtt
    20281 cgctgaactg gctgatacca aacttttcct gatcgagtag ttcgaaaccg accgcgccgc
    20341 ctttgggcaa accttgttgt gccaggcgca gacgcagttc gtgaacttta tctgccggaa
    20401 cttcaatagc gccgctggct tcgctgaagc ggtaaggaat attcatttgc gtcagttggc
    20461 tgacaattgc gccaccatcc tgatcggaaa gattgctgaa taatgtgcgg tagtcggggg
    20521 ctttcgccca caggatcagt gcgaccatga ccgccactgc cgcggaaccg gcaacaatca
    20581 atggaatttt cggattcgca cgcaggcgat taagccactc aagagatttt gtctgggctg
    20641 cagtcgcatt catcgcgcac ctcgtggctg attatggacg gcgttattgg caaagtggaa
    20701 caagactcac tcccgggtca gcaaactcga aaaattgacg ctgccattat ttgtgctttc
    20761 gtttttttct atccgccaat aaacccgttt ttttgttgct atttagcgcc tttgtcttat
    20821 tgacttactg gtaggctttg ctaccagaaa ttatccagga gacgaaaatg tcagcgatac
    20881 aggggattga aggggttata agccagttac aggctacggc gatgagtgcg cgtgcgcagg
    20941 attcactgcc gcaaccgacc attagttttg ccgggcagct gcacgccgcg ctcgatcgca
    21001 ttagcgatac acaaacagct gcccgcacgc aggcagaaaa attcaccctc ggtgaacccg
    21061 gcgtggcgtt aaacgatgtg atgaccgata tgcaaaaagc ctcagtttct atgcaaatgg
    21121 ggattcaggt gcgtaataag ctggtggcgg cgtatcagga agtgatgtcc atgcaagtgt
    21181 aggttttgta acctgttgtt aattacatcc catctgcccg cgtggggcat ggatggggca
    21241 aactcaccct atttctggtt taaaatggat acctgatcct gaaagttttc tgccatccac
    21301 gatccgtata cgcgataaca tgttttgtct tcgctttgct cataggaaga gtgaactctt
    21361 ttgttagcct ggggttcctt ctgatcatta ttgttcctgc ctttatctct tgccacgcca
    21421 gagccccctg gatacacata caccaggatg aaaacggtga gttgtgttca aactgtagta
    21481 caattctctc cagtatgaac aggaaagaat atgctatgaa cccttatatt tatcttggtg
    21541 gcgcaatact tgcagaggtc attggtacaa ccttaatgaa gttttcagag gggtttacac
    21601 ggttatggcc ctctgttggc acaattattt gctattgtgc atcattttgg ttattagctc
    21661 agacactggc ttatatacct acagggattg cttatgctat ctggtcagga gtcggtatcg
    21721 ttctgattag tttgctttcc tggggatttt ttggccaacg actggacttg ccagccatca
    21781 taggcatgat gttgatttgt gccggtgtgt tagtcattaa cttgttgtca cgaagcgcac
    21841 cgcattaaaa aataatcatc cttgagataa caggaatctg atttgtgcat attatggcta
    21901 atgttcgggg caactttata attatgattg catttgcgcg atcattgcgc ctttttgcat
    21961 aatgagatca atgcacatca aataaaataa cgataggtca caacaaagag gtttttatga
    22021 aaaaacttat cgtttcaagt gtattggcat tcataacatt ttctgcgcag gccgcagcat
    22081 ttcaggtcac tagtaatgaa ataaaaacag gagagcaact tacaacgtct catgtatttt
    22141 ctggatttgg gtgtgagggg ggtaatacat cgccctcatt aacctggtct ggtgctccag
    22201 aaggcaccaa aagctttgcc gtaactgtgt atgatccaga tgcgcctaca ggcagcggat
    22261 ggtggcactg gactgttgct aatattccag caacggtaac atatttgccc gctgatgcag
    22321 gaagacgtga tggtacaaaa cttccgactg gtgctgtcca aggccgaaat gattttggct
    22381 atgcgggatt tggtggagca tgtcctccca aaggagataa accccatcat taccagttta
    22441 aaatatgggc tctaaaaact gataagattc ctgtggattc taattccagc ggagcgttag
    22501 ttggttatat gcttaatgcc aataaaattg caaccgctga gataataccc gtttatgagg
    22561 taaagtagga taagagtatg ctggcaaaag ataggactaa cctaaagatt gaagaaatac
    22621 ggatgcataa acatcatgag attcacaggg ttaagcctct tatgccagct ttatgtcgta
    22681 tccgtcaggg aaaaaaagtt atcaattggg agacgcatag tctaactgtt gataataatc
    22741 agataatatt atttccctgt ggatatgaat tttatattgc gaattaccca gaagcaggtc
    22801 tttatcttgc agaaatgctt tattatccca ttgatctaat tgaaaagttc caaaatcttt
    22861 atgcgataac cgatcaaatt cgtaacacga caggtttctg cttacctcag aatcccgagt
    22921 taatatattg ttgggagcaa ctaaaaacat ctatttcccg aggcttctca acgcaaattc
    22981 aggaacactt agcaatgggc gttctacttt cattaggagc acatcatgtt aattgtttac
    23041 ttttatcaga tagtaaacaa tcattaataa gtcgttgtta taacctaatg ctatccgaac
    23101 ctggaacaaa atggacagca aacaaggtag cgagatatct ctacatttct gtttccacat
    23161 tgcatcgccg tctggcaagc gagggagtaa gttttcaaag tatattggac gacgtgaggt
    23221 taaataatgc gttgtctgct atacaaacga cagtaaaacc catcagcgag attgccaggg
    23281 aaaatggtta caagtgtcct tctcgtttta ctgaaaggtt ccataatcgt tttaagataa
    23341 caccaagaga gctaagaaaa gcgtccagag agtaaaagta ttttatgaag gagcaacagc
    23401 atcgattttt attttggaaa ataaatacgg catactcaac ctattggagc ctgaacaatc
    23461 cggtgacttc tgcgctaatc ggggacgttt ataagcacat ataattgata ctatcgtcct
    23521 ccatcacaat ttcagaaatg cacgtgtgat tttttgcatc cggcgtttgg actatgtgga
    23581 gttcaagagt gggaaagtta caagaggttg aggggataat taaagggatg cttatgacat
    23641 tagatatccg gataggtacg gatatcgttg ttattaaatc tagtttgttg ttattaaatc
    23701 tagttcgttg ttattaaatc tggtttatac g
//
LOCUS       DAZGAN020000068        23595 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGAN020000068 DAZGAN020000000
VERSION     DAZGAN020000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.068"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            357..1490
                     /gene="ompN"
                     /locus_tag="ACHKYO_RS15080"
                     /old_locus_tag="pgaptmp_003016"
     CDS             357..1490
                     /gene="ompN"
                     /locus_tag="ACHKYO_RS15080"
                     /old_locus_tag="pgaptmp_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HGU4429829.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLINGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1631..2065
                     /gene="uspF"
                     /locus_tag="ACHKYO_RS15085"
                     /old_locus_tag="pgaptmp_003017"
     CDS             1631..2065
                     /gene="uspF"
                     /locus_tag="ACHKYO_RS15085"
                     /old_locus_tag="pgaptmp_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HGU4429830.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            2241..3176
                     /gene="ttcA"
                     /locus_tag="ACHKYO_RS15090"
                     /old_locus_tag="pgaptmp_003018"
     CDS             2241..3176
                     /gene="ttcA"
                     /locus_tag="ACHKYO_RS15090"
                     /old_locus_tag="pgaptmp_003018"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HGU4429831.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3305..4678)
                     /gene="dbpA"
                     /locus_tag="ACHKYO_RS15095"
                     /old_locus_tag="pgaptmp_003019"
     CDS             complement(3305..4678)
                     /gene="dbpA"
                     /locus_tag="ACHKYO_RS15095"
                     /old_locus_tag="pgaptmp_003019"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HGU4429832.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSILPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGVDIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4708..4881)
                     /gene="ynaL"
                     /locus_tag="ACHKYO_RS15100"
                     /old_locus_tag="pgaptmp_003020"
     CDS             complement(4708..4881)
                     /gene="ynaL"
                     /locus_tag="ACHKYO_RS15100"
                     /old_locus_tag="pgaptmp_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HGU4429833.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(5156..6139)
                     /gene="zntB"
                     /locus_tag="ACHKYO_RS15105"
                     /old_locus_tag="pgaptmp_003021"
     CDS             complement(5156..6139)
                     /gene="zntB"
                     /locus_tag="ACHKYO_RS15105"
                     /old_locus_tag="pgaptmp_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HGU4429834.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(6153..6200)
                     /gene="ydaN"
                     /locus_tag="ACHKYO_RS15110"
                     /old_locus_tag="pgaptmp_003022"
     CDS             complement(6153..6200)
                     /gene="ydaN"
                     /locus_tag="ACHKYO_RS15110"
                     /old_locus_tag="pgaptmp_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HGU4429835.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6394..7626
                     /gene="dgcM"
                     /locus_tag="ACHKYO_RS15115"
                     /old_locus_tag="pgaptmp_003023"
     CDS             6394..7626
                     /gene="dgcM"
                     /locus_tag="ACHKYO_RS15115"
                     /old_locus_tag="pgaptmp_003023"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HGU4429836.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7647..8210)
                     /gene="smrA"
                     /locus_tag="ACHKYO_RS15120"
                     /old_locus_tag="pgaptmp_003024"
     CDS             complement(7647..8210)
                     /gene="smrA"
                     /locus_tag="ACHKYO_RS15120"
                     /old_locus_tag="pgaptmp_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HGU4429837.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8540..9448)
                     /gene="abgR"
                     /locus_tag="ACHKYO_RS15125"
                     /old_locus_tag="pgaptmp_003025"
     CDS             complement(8540..9448)
                     /gene="abgR"
                     /locus_tag="ACHKYO_RS15125"
                     /old_locus_tag="pgaptmp_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4429838.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9624..10934
                     /gene="abgA"
                     /locus_tag="ACHKYO_RS15130"
                     /old_locus_tag="pgaptmp_003026"
     CDS             9624..10934
                     /gene="abgA"
                     /locus_tag="ACHKYO_RS15130"
                     /old_locus_tag="pgaptmp_003026"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HGU4429839.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10934..12379
                     /gene="abgB"
                     /locus_tag="ACHKYO_RS15135"
                     /old_locus_tag="pgaptmp_003027"
     CDS             10934..12379
                     /gene="abgB"
                     /locus_tag="ACHKYO_RS15135"
                     /old_locus_tag="pgaptmp_003027"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HGU4429840.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            12413..13939
                     /gene="abgT"
                     /locus_tag="ACHKYO_RS15140"
                     /old_locus_tag="pgaptmp_003028"
     CDS             12413..13939
                     /gene="abgT"
                     /locus_tag="ACHKYO_RS15140"
                     /old_locus_tag="pgaptmp_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HGU4429841.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDVLESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13950..14465
                     /gene="ogt"
                     /locus_tag="ACHKYO_RS15145"
                     /old_locus_tag="pgaptmp_003029"
     CDS             13950..14465
                     /gene="ogt"
                     /locus_tag="ACHKYO_RS15145"
                     /old_locus_tag="pgaptmp_003029"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGU4429842.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            14660..15412
                     /gene="fnr"
                     /locus_tag="ACHKYO_RS15150"
                     /old_locus_tag="pgaptmp_003030"
     CDS             14660..15412
                     /gene="fnr"
                     /locus_tag="ACHKYO_RS15150"
                     /old_locus_tag="pgaptmp_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HGU4429843.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            15564..16514
                     /gene="uspE"
                     /locus_tag="ACHKYO_RS15155"
                     /old_locus_tag="pgaptmp_003031"
     CDS             15564..16514
                     /gene="uspE"
                     /locus_tag="ACHKYO_RS15155"
                     /old_locus_tag="pgaptmp_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HGU4429844.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(16564..16821)
                     /gene="ynaJ"
                     /locus_tag="ACHKYO_RS15160"
                     /old_locus_tag="pgaptmp_003032"
     CDS             complement(16564..16821)
                     /gene="ynaJ"
                     /locus_tag="ACHKYO_RS15160"
                     /old_locus_tag="pgaptmp_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HGU4429845.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            17065..18096
                     /gene="ynaI"
                     /locus_tag="ACHKYO_RS15165"
                     /old_locus_tag="pgaptmp_003033"
     CDS             17065..18096
                     /gene="ynaI"
                     /locus_tag="ACHKYO_RS15165"
                     /old_locus_tag="pgaptmp_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HGU4429846.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPQEQGR"
     gene            complement(18147..19760)
                     /gene="mppA"
                     /locus_tag="ACHKYO_RS15170"
                     /old_locus_tag="pgaptmp_003034"
     CDS             complement(18147..19760)
                     /gene="mppA"
                     /locus_tag="ACHKYO_RS15170"
                     /old_locus_tag="pgaptmp_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HGU4429847.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(20097..20996)
                     /gene="pgrR"
                     /locus_tag="ACHKYO_RS15175"
                     /old_locus_tag="pgaptmp_003035"
     CDS             complement(20097..20996)
                     /gene="pgrR"
                     /locus_tag="ACHKYO_RS15175"
                     /old_locus_tag="pgaptmp_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HGU4429848.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSAMASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHCRHAGSAFSLFIDRLKYKGAV"
     gene            21134..22054
                     /gene="ycjY"
                     /locus_tag="ACHKYO_RS15180"
                     /old_locus_tag="pgaptmp_003036"
     CDS             21134..22054
                     /gene="ycjY"
                     /locus_tag="ACHKYO_RS15180"
                     /old_locus_tag="pgaptmp_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4429849.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVLSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            22054..22986
                     /locus_tag="ACHKYO_RS15185"
                     /old_locus_tag="pgaptmp_003037"
     CDS             22054..22986
                     /locus_tag="ACHKYO_RS15185"
                     /old_locus_tag="pgaptmp_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4429850.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            23004..>23595
                     /locus_tag="ACHKYO_RS15190"
                     /old_locus_tag="pgaptmp_003038"
     CDS             23004..>23595
                     /locus_tag="ACHKYO_RS15190"
                     /old_locus_tag="pgaptmp_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4429851.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGDNI"
BASE COUNT         6166 a         5711 c         5633 g         6085 t
ORIGIN      
        1 taaggattat ccaattctaa aaaaaaagcc cggacgactg ttcgggcttg tctttttata
       61 tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag
      121 gcatataacc cgcgcagaat aacgtatagc aaattaaaag tataattttt attttgtatg
      181 tattatcttc gatacattca ccaaacttat tatcatttca gaggaattat ttgattaagg
      241 ttttacttaa ggcgtaacaa atgatattta tcggctaact gaacttctcc tttaggatgt
      301 tttcactccc cgaaatgggg ataaaaggca aataaaataa ctaaaggatt tattcaatga
      361 aaagcaaagt actggcactt ttaattcctg ccctgctcgc cgcaggtgct gcacatgcag
      421 ccgaagttta taataaagac ggcaacaaat tagatctgta tggcaaagtt gatggcctgc
      481 attatttttc tgataattcg gcgaaagatg gcgaccagag ctatgcgcgt ctgggtttta
      541 aaggcgaaac ccaaattaac gatcaactca ctggctacgg tcaatgggaa tacaatattc
      601 aggcaaacaa cactgaatct tcaaaaaacc agtcatggac ccgtctggca tttgccgggc
      661 tgaaatttgc agattacggt tctttcgatt acggacgtaa ttatggcgta atgtacgaca
      721 tcgaaggctg gaccgatatg ctgcctgaat ttggcggtga ctcttatacc aatgcagaca
      781 actttatgac tggtcgagcc aatggcgtcg cgacttatcg taatactgat ttcttcggtc
      841 tgataaatgg tctgaacttc gcggtgcagt atcaaggtaa caacgaaggt gccagtaatg
      901 gtcaggaagg caccaacaac ggacgtgatg ttcgccatga aaacggtgac ggctggggtc
      961 tttccacaac atatgattta ggcatgggct ttagcgctgg tgcggcatac acctcttctg
     1021 accgcaccaa tgatcaggtt aaccatactg cggcgggtgg tgataaagca gacgcgtgga
     1081 ctgctgggct aaaatacgat gctaacaata tttacctggc aaccatgtat tcagaaacgc
     1141 gtaatatgac cccgtttggc gacagcgatt atgctgttgc aaacaaaacc cagaactttg
     1201 aagtcactgc acagtaccag tttgattttg gtctgcgtcc ggcagtctct ttcctgatgt
     1261 ctaaaggccg tgacctgcac gctgccggtg gtgcagacaa cccggcaggt gttgatgata
     1321 aagatctggt taaatacgcc gatgttggcg cgacttacta tttcaataaa aacatgtcca
     1381 cttatgttga ctataaaatc aacctgttgg atgaagatga cagtttctac gctgccaatg
     1441 gcatctctac cgatgatatt gtcgctttag gtctggttta tcagttctaa atcctcctgc
     1501 ccgctgttat ggcgggcttt ttctgcttat tcttcctcct ttgatctaaa ttaaaaatgt
     1561 gaactccgtc attacacaaa aagtgtcatc tggcgttaca ctttatgcgg atactaaaac
     1621 aggaggtttt atgaacagaa cgattcttgt ccctatcgat atttccgatt cagaattaac
     1681 tcaacgcgtg attagccacg ttgaggcaga ggcaaagatt gatgatgcag aggttcattt
     1741 cctgacggta ataccttcac tgccctacta tgcctctctg ggtttagcgt attccgcaga
     1801 attaccggca atggatgacc tgaaagcgga agccaaatcg caactggaag agatcattaa
     1861 aaaatttaaa ctgccaaccg acagagtgca tgtccatgtt gaggaaggct cgcccaaaga
     1921 ccgcattctg gaaatggcga agaagatccc cgctcatatg atcatcattg cttcccatcg
     1981 accggatatc accacttatc tgctcggttc caacgccgca gctgtagtgc gtcacgcaga
     2041 gtgctccgtg ctggttgtgc gctgacacta acgcccgcac attgctgcgg gctttttgat
     2101 tcatttcgca aatgtgctga cattttcccc tctaatccgt accatacacg ccactgtttt
     2161 tatatcagat ttcttatgct gggcattccg gcatgacgca tactcttctg atgccatata
     2221 acgaattgag tcgcttttaa atgtcgcaaa atcaagaaat tagtaagaaa gaacaataca
     2281 acctgaacaa attacaaaaa cgtctgcgtc gtaacgtggg cgaagccatt gctgacttca
     2341 atatgattga agaaggcgat cgcatcatgg tttgcctctc cgggggtaaa gacagctata
     2401 ccatgctgga gattctgcgc aatttgcagc aaagcgcgcc aatcaatttt tcgctggtgg
     2461 ctgttaacct cgatcaaaag caaccgggct tcccggaaca cgttctgccc gagtatcttg
     2521 aaaagctggg cgttgagtac aagattgttg aagagaatac ttacggtatc gtgaaagaga
     2581 agattccaga gggcaaaacc acttgctcac tgtgttctcg ccttcgtcgc ggtatccttt
     2641 atcgtaccgc aacggaactg ggggcgacga agatcgcgtt gggtcaccat cgtgacgata
     2701 tcctgcaaac gttgttctta aatatgttct acggcggcaa gatgaaaggt atgcctccga
     2761 aactgatgag cgatgatggc aaacatatcg ttattcgtcc gctggcctac tgccgcgaga
     2821 aagatattca gcgatttgcc gatgcaaaag cgttcccgat tattccgtgc aacctgtgcg
     2881 gttcacagcc taacctgcaa cgtcaggtga ttgctgacat gttgcgtgac tgggataaac
     2941 gttatccagg gcgtatcgag acgatgttca gcgcgatgca gaatgtggtg ccgtcgcatc
     3001 tgtgcgatac caacctgttc gatttcaaag gcattaccca cggttctgaa gtggttaacg
     3061 ggggtgatct ggcgtttgat cgcgaagaga tcccactaca accggcgggc tggcagccag
     3121 aagaagatga aaatcagttg gatgagttac ggctgaatgt ggttgaagtg aaataaccag
     3181 gatagcgccc gatgcgcaag cgtatcgggc tactcttatg gaggccggat aagacgcggc
     3241 cagcgtcgca tccggcaatc ccgcataaga tgtttactct tgcacccggc aattcaacat
     3301 ttcattattt taataaccgc acccggcacg tttttccttt aatcttcccg ccctgtaact
     3361 gtttccatgc tttatgagca acagcctgac ggaccgcgac atagacatgc gccggatgca
     3421 cggcgatttt gccaatatct acgccatcaa gcccgatgtc tcctgtcagc gcacctaata
     3481 catcacccgg gcgcattttg gcttttttcc cgccatcgat acacaacgtt gccatttctg
     3541 cttccagcgg caaaatggaa ctattagctg gcggcgtttg ccagttaagt tttatctgca
     3601 acatgtcaga aatgatattg gcccgctgtg cttcttccgg agcacagaaa ctgatcgcca
     3661 gaccgctatt tcctgcacga gctgtacgac cgatgcgatg tacatgaact tcagggtccc
     3721 acgccagctc aaagttcacc accagctcaa gcgatttaat atccagacca cgcgcagcaa
     3781 catcagtcgc aaccagtaca cgggcgctac cgttagcaaa acgtaccagg gtctgatcgc
     3841 gatcgcgttg ctccagatcg ccgtgtaacg acaatgcact ttgccctact tcattcagcg
     3901 cgtcgcagac agcctggcaa tcttttttgg tattgcaaaa caccacacaa gaggatggct
     3961 gatgcaaact taataaccgt tgcaacagag gaattttgcc tttactggat gtctcataaa
     4021 attgttgttc aatgggtggc aaagcatctg ttgagtcaat ttcaatcgcc aaaggatcgc
     4081 gttgcactcg tccgctgatt gcggcgatgg cttccggcca ggttgccgaa aacagaagcg
     4141 tctgtcgaga tgcaggcgca aaacggatga catcatcaat ggcatcgcta aatcccatat
     4201 ccagcatgcg gtcggcctca tccatcacca aagtattcag cgcatccagt gataccgtgc
     4261 ctttttgcaa gtgatccagc aaacgacccg gcgttgccac gataatatgc ggcgcatgtt
     4321 gcaacgaatc acgctgcata ccgaacggtt gaccaccgca caacgtcaaa attttggtat
     4381 ttggcagaaa acgcgccagc cgacgcaatt cacctgccac ctgatccgcc agttcacgcg
     4441 taggacacag cactaaagcc tgggtttgaa atagcgacgc atcaatttgc tgtaacaagc
     4501 cgaggccaaa agccgccgtt ttgccgctgc cggttttcgc ctgcacgcga acatcttttc
     4561 cggcaaggat cgccggaagc gcggcggcct gcaccggcgt catggttaaa taacccaact
     4621 cattaaggtt cgtgagttgg gcgggaggca aaacattcag ggtagaaaaa gcggtcacaa
     4681 tctattctcg tggtcatcga cgcaaagtta gcaggcgcgt atcctcgcag atctacgctc
     4741 acgatgcgac aatttaatcg gttcttcatc aggtggtggg tcaggcatgg gttgcgggcg
     4801 agggatcgga tcgggcactg gaacaggatc gccaggaatc ggttcaggga caggaatttg
     4861 caaataaata agtgtcgtca tatttccctc cggtcattgg gtggactctt aaagggtaga
     4921 cgctgataaa taacaggcaa aaaaaagccg actcatcaaa gtcggcgtcg tacgaatcaa
     4981 ttgtgctatg cagtaattca aaaaaggaag taagacaata tggagcgcaa cgcccatcgc
     5041 ttgacgttgc attcacctgc aagggagata ttgccctgaa tgggtagaga gtttattgac
     5101 ttcgctcaaa ctttgcggcg tttttgtata cagacagccg gaaaaattgc ttttgttaca
     5161 accatttact acggtgcaac cataaagcaa caccaccaat aagaacaact aacagaatac
     5221 aaaaaattga aaatccgaat tgccacccgc cgccagggat cccaccaagg ttgacgccaa
     5281 ataaccctgt cagaaaggta ctgggtaaaa agaccattgc catcaatgac attgtatagg
     5341 tacgacgagc taaattttcc tgcatcacct gagcgatttc atccgccatc acgccagtcc
     5401 gtgctataca ggcgtcgatt tcgtcaagac cgcgcccaag gcgatcggca atatcctgca
     5461 tccgacggcg ttggtcatcg ctcatccacg gcaaacgttc actggcaaga cgagcataaa
     5521 catcacgttg cggtgccata tagcgacgca tcacgattaa ttgtttgcgc agcagagcca
     5581 ggaatccacg cggtggaatt tgctgatcaa ggagattatc ttcaaggtcg ataattttat
     5641 cgtgcagctg ctcgataaat tcgctggaat gatcggtcaa cgcatcgcac acatccacca
     5701 gccaccctcc gcaatcggtc ggacccgtgc cctcttccag atcgctcacc acatcgtcca
     5761 gcgccagcac tttgcgttgt cgggtcgaaa caattaaccg cccgtccata tatacacgca
     5821 tggcgaccag ttgatcgggg cgttcatcgg tgctgccgtt tatacagcgc aatgtaataa
     5881 gcgtgccttc accgagacgg ctgactcggg gccgtgtgct ctcgcccgcc agcgcatcac
     5941 gtacgttatt gggaagcagc ggtgttgtcg ccagccattg ggcgctatca tggtgtacat
     6001 aattaaggtg gagccagcag ggatgcgctt catcaatcac atctgtattt tccagcggtt
     6061 taacgccgcc tctaccatcc agcatccagg caaatactgc atccgggaca ttaacgtccg
     6121 atcccttaat cgcttccaca gtgcctccat catcaacgca ttattttgta gtctagcctt
     6181 ctggccctgt tacgcaacat ctcatcaccc cattaccctg aaatgattaa taaaattctg
     6241 tctaaattga atacaaaaag caaaatgctt ttccgtatac aaaccgtgtg aagtgttaaa
     6301 tagcgtctat cattatcaga attatctgat catatgacgt ggcttttttg cgatcggata
     6361 gcaacaaaaa ttgataaaaa taacgggatc tcaatgatta cgcacaactt caataccctg
     6421 gacttactca ccagtcctgt ctggattgtt tcgccctttg aggaacagtt aatttatgcc
     6481 aatagcgcgg cgcgactgtt gatgcaagac ctcacgttta gtcagctacg aaccggaccc
     6541 tattccgtct cctcacaaaa agaactgccg aaatacctct ccgatctgca aaaccaacac
     6601 gatattatcg aaatcctcac tgttcagcgt aaagaagagg aaacagcatt aagctgtcgg
     6661 cttgttttgc gagagctgac agaaacagaa ccggtgatta ttttcgaagg tatcgaagcg
     6721 ccggcaacgc tgggtttaaa agccagtcgc tcggcaaatt atcagcgcaa aaaacaaggt
     6781 ttttatgcgc gcttttttct gactaactct gcaccaatgt tgttgattga cccctcacga
     6841 gatggccaaa tcgtcgatgc taacctcgcc gcgctcaatt tctatggtta taaccatgaa
     6901 acgatgtgcc agaaacatac ctgggaaatt aatatgctcg ggcgtcgcgt catgcctatc
     6961 atgcatgaaa tttcgcattt acccggcggt cataaacctt tgaattttgt tcataaactg
     7021 gcggatggat ctactcgtca tgtgcagacc tatgccgggc cgattgaaat ttatggcgac
     7081 aagctcatgt tatgtattgt gcatgatatt actgagcaaa aacggctgga ggagcagctg
     7141 gaacatgctg ctcaccatga cgcgatgacc ggattactga atcggcgaca gttttatcac
     7201 attaccgagc caggccaaat gcagcatctc gccatcgctc aggattacag cttgttgctc
     7261 atcgacaccg atcgttttaa acacattaac gatctctatg ggcattctaa aggtgatgag
     7321 gtgttatgcg ccctcgcccg cacccttgaa agttgcgctc gcaaaggcga tttggtgttt
     7381 cgttggggag gcgaagagtt tgtcttattg ctaccaagaa ccccactgga taccgcgctt
     7441 tcgctggctg aaactatccg tgtaagcgtg gcaaaagtga gtatttcggg cttaccacgc
     7501 tttaccgtca gcattggtgt ggcgcatcac gaaggaaatg aaagcatcga tgaactgttt
     7561 aaacgcgttg atgatgcttt gtatcgggcg aaaaatgatg gacgcaaccg cgtgctggcg
     7621 gcataagccg cggatgcgtc tcgagatcaa cgactgcgct tagcgtggcg ctcccagttt
     7681 tcttgcttcg cctgcgccgt tttacgtagt gcgacgtaac acgccccgct gccgccatga
     7741 tgcggtagcg cagtgcaata tgcctgaaca tcatcaaatt cggtcagcca gcgcgccaca
     7801 tagctgcgga caatattggc atgcgattta tcatcccgtc ctttgccatg aataatcagc
     7861 acgttacgca aaccatcagc catggcttgt tgaatgaaac tgaacatcat tttgcggcac
     7921 tcttccaccg gctggcgcaa aagattcagg ctcgcctgtt gcggatattt accactgcgc
     7981 agcttatcca gcaccccatg ttgcaaccct tcccgccgaa actccagcgg ctgacttagt
     8041 gggatgatgt cgagaaatcc ggtggtgagg aaattatcaa gctgcagcgt gtcgatacgc
     8101 tgcggcgcac gttggttacg cgttggatgc cagtggacat cggtagcacg tttcagcggc
     8161 tggacatctt ccatggcgtc aagaaacagc gatttgtcgt caaggttcat gttacatcct
     8221 cccgcaatta agagcgcgat atgataacca gaccgggtcg gtccaacaac gtattaccca
     8281 aaattccagt aataagttcc aaatattgcc gatattttaa gcaaaatgct tatgcatgat
     8341 tattcattca cgatattaat aatgtaactt atattttcgt gaaatctgtc actgaagaaa
     8401 attggcaact aaaggttaaa accgttataa cacagtcacc ggcgcagagg agacaatgcc
     8461 ggacttaaga cgcggatgca ctgctgtgtg tactgtagag tctggcggat gtcgacagac
     8521 tctatttttt tatgcagttt taactttgca gatagccgca ttctcgccgg aattgcgtga
     8581 ttaatgacgc ggtcagtggt gtctggcgac tatcacgccg ctgaatcaaa taataggccg
     8641 ctttcggtaa aatttcgcta accggcaaca tcaccagccc ctgtccgtgc aagggatcgc
     8701 agcccatttc ttcaggcagt atgctgagaa aatcgctttt tgccaccaga ctgatacagg
     8761 ctgagaacgt ctcgcagact acaccgacct gtggcgtttg cgcctgatcg tcaagcaatt
     8821 cactcaactg tttgtagtaa ctgccgtgtg gcgtcggcat tgtccagctg taatccagta
     8881 actgtttgat cgaacgggca ccaatggcgg ggtgtcccgg gcggcaaaag atcgcgaatt
     8941 gcttttccag taatttctca aaagtaaatt cgtggtcgta cggtccctga taataggtat
     9001 tgatggtgaa atccaattct ccctgacgca attcattaat catcgacacc agttgccctt
     9061 ccataatgcg tacttttacc tgcggatgct gctgatgaaa acgagatatg acagctggca
     9121 tcagactgcg ggaaatactg gcccccatgc cgatattaat ctgccctgcc agttgccctt
     9181 gtcgttggcg aatttcctct tgcgctgcgc gcagctcttc aagaataaga ctggcgtgct
     9241 gataaaaact ttcaccggca tcagttaacg tcacgccttt actacggcga aaaaagagtt
     9301 gcgccgctaa tccttcttcc agctcctgaa tagatttact cagtgccggt tgcgacatat
     9361 tcaacattcg gctcgctccg cgaatgctgc cctgacgagc cacttcaaca aaagcacgaa
     9421 tttgatgaat ttttacctga aaagccatga cgccaccgat aaccgttatt tatcagacca
     9481 aagaaactgg catctacttt aatgcagatg attgtgtcag ggtaatttat gaacggttaa
     9541 aactgtgaaa aatcagttag tgataagtaa aaactatcgc tacgtgaacc gggtcacact
     9601 ttttactgat gacgggaaag gttatggagt ctttgaatca atttgttaat tcgcttgccc
     9661 caaaattatc gcactggcga cgtgattttc atcactatgc agagtctggc tgggtggaat
     9721 tccgcactgc cacccttgtt gcggaagaat tgcagcagct cggctattca ctggcgctgg
     9781 gccgcgaagt agttaatgaa agtagccgga tgggactacc tgatgaactc actctacaac
     9841 gcgaattcga gcgcgctcgt caacagggtg cgctagcaca atggattgcg gtttttgaag
     9901 gtggtttcac tggtatcgtc gccaccctgg ataccggtcg ccccggtccg gtgatggctt
     9961 tccgtgtcga tatggacgcg ctggatctca gtgaagagca ggatgtcagc catcgcccct
    10021 accgcgacgg ttttgcgtca tgtaacgccg gaatgatgca tgcctgtggt catgatggac
    10081 ataccgccat tgggcttggg ctggcgcata cccttaaaca attcgagtcc ggactacatg
    10141 gcgtcatcaa actgattttt cagcctgcag aggaaggtac gcgtggcgcg cgggcgatgg
    10201 tcgatgcagg tgtcgtagat gatgttgatt attttactgc cgtgcacatt ggcactggcg
    10261 tacctgcggg caccgtggtg tgcggcagtg ataattttat ggcaaccacc aaatttgacg
    10321 cgcacttcac cgggaccgcc gctcacgcag gcgcaaaacc agaagacggt cacaatgcct
    10381 tgttggcggc agcacaagca actcttgcac tgcatgcaat cgccccgcac agcgaaggag
    10441 cttccagagt aaacgtgggc gttatgcagg caggaagcgg ccgtaacgtt gttcctgcct
    10501 cggcgttgct gaaagtggaa acacgcgggg ccagcgacgt cattaatcaa tatgtttttg
    10561 accgtgcaca acaagcgatt cagggcgcag caaccatgta tggtgtcggc gttgaaactc
    10621 gtctgatggg tgcagctacc gccagttctc cttcgccgca atgggtcgca tggttgcaaa
    10681 gtcaggcggc tcaggtcgcg ggggtcaatc aggccattga acgtgttgaa gcgcctgcgg
    10741 gttccgaaga tgccacatta atgatggccc gcgtgcagca acatcaaggg caagcctcct
    10801 acgtggtgtt tggcacacag ctggcggcag gtcatcacaa cgaaaaattc gattttgacg
    10861 agcaggttct cgctattgcc gtcgaaacgc tggcgcgcac cgcgctcaat tttccctgga
    10921 cgcgaggtat ctgatgcagg aaatctatcg ttttatcgac gatgcgattg aagccgatcg
    10981 ccaacgttat accgatattg ccgatcaaat ctgggatcat ccagaaacac gttttgaaga
    11041 gttctggtca gcggagcatc tggcttcggc gctggaatct gcaagcttca ccgttacccg
    11101 caacgtaggc aatatcccaa atgcatttat tgcttcgttt ggtcaaggca aaccggttat
    11161 cgccctgctg ggagaatatg acgccctggc aggtttaagt cagcaagctg gttgcgcgca
    11221 acctacatcc gcgacgcccg gtgaaaatgg tcacggttgc ggacacaatt tgctgggaac
    11281 cgccgccttt gccgctgcaa tagccgtcaa gaaatggctg gaacaatatg ggcaaggcgg
    11341 cacagtgcgc ttttatggtt gtcctggcga agaaggcggc tcgggtaaaa cgttcatggt
    11401 ccgcgagggg gtatttgatg atgtggatgc ggcactcacc tggcacccgg aagcctttgc
    11461 cggcatgttc aatacccgca cgctggcaaa cattcaggca tcatggtgct ttaaagggat
    11521 cgcagcacat gccgcgaatt cccctcattt gggacgcagc gcccttgatg ccgtaacgtt
    11581 gatgaccact ggcaccaact tcctcaacga acatattatt gaaaaagcgc gcgtacacta
    11641 tgccatcaca aatagcggcg ggatctcgcc caacgtggtc caggcgcagg cagaagtgct
    11701 ttatcttatc cgtgcccccg aaatgaccga cgtgcagcat atttatgatc gggtcgccaa
    11761 aatcgccgaa ggtgcggcat tgatgaccga aaccacggtt gaatgccgct tcgacaaagc
    11821 ctgttccagt tatctcccga atcgcacctt agaaaatgcc atgtaccatg ccctatccca
    11881 ttttggtacc ccggaatgga actccgaaga actggctttt gcgaaacaaa ttcaggctac
    11941 gctcaccccc aacgatcggc aaaacagtct gaataatatc gccgcaaccg gtggcgaaaa
    12001 cggcaaggtt tttgcactac gtcatcgtga aacggtactg gcgaatgagg tcgctccata
    12061 tgccgccacc gataacgtgc ttgcggcatc gactgatgtc ggcgacgtca gttggaaact
    12121 gcctgttgcc cagtgtttca gcccctgttt tgccgtcggt acaccgctac atacgtggca
    12181 actggttagc caggggcgaa catctattgc tcataaagga atgctgctgg cggcgaaaac
    12241 tatggcagca accacagtca atctcttcat tgattcaggg ctattgcaag aatgccaaca
    12301 agagcatcag caagttacgg acacgcaacc gtatcactgc cctatcccga aaaacgtgac
    12361 accgtcacct ttaaaataac aacaacgcaa acacaacaac cgaggaatgc ccatgagtat
    12421 gtcatccata ccgtcgtcct cccaatccgg gaagctctat ggctgggtcg aaagaattgg
    12481 taacaaggtt ccccatcctt ttttgctctt tatctatttg ataatcgtac tcatggtgac
    12541 gacggcaatt ttgtcagcct ttggcgtcag tgcgaaaaac ccgaccgatg gtacgccggt
    12601 ggtggtgaaa aacctgctca gtgtggaagg attacactgg tttttaccca atgtgattaa
    12661 aaactttagc ggttttgctc cacttggagc gatcctggcg ctggttttag gtgccggcct
    12721 agcggagcgc gtcggcttac tgccagcgct aatggttaaa atggcatcgc atgttaatgc
    12781 ccgctacgcc agttatatgg tgctgtttat tgcttttttc agccacattt cttccgatgc
    12841 ggcgttagtg atcatgccac cgatgggtgc gctgattttt ctggcggtgg gcaggcatcc
    12901 agttgcaggt ttactggctg ccattgcagg cgtaggttgc ggctttacgg ctaatttact
    12961 gattgtcaca accgacgtgt tgctgtcggg gatcagcacg gaagcggcgg ctgcgttcaa
    13021 tccgcaaatg cacgtcagtg taattgataa ctggtatttt atggccagct ccgtagtcgt
    13081 actgacgatt gttggcggct tgataaccga caaaatcatc gagccacggt taggtcaatg
    13141 gcagggaaac agcgatgaga aactgcagac attgaccgaa agtcagcgtt ttggtttacg
    13201 catagcaggt gtcgtatcgc tactttttat tgctgcgatc gcgctgatgg tgatcccgga
    13261 aaacgggata ttgcgcgatc cgattaatca caccgtgatg ccatcaccct ttattaaagg
    13321 tatcgtgcca ctaatcattc tttttttctt tgtggtttcg ctggcttatg gcatcgctac
    13381 ccgcacaatt cgacgtcagg cggatttacc gcatttaatg attgaaccga tgaaagagat
    13441 ggcgggattt atcgtgatgg tttttcccct cgcccagttt gtcgccatgt ttaactggag
    13501 caacatgggg aaattcatcg ccgtggggct gaccgatgta cttgaaagtt cagggcttag
    13561 cggcatcccg gcgtttgtcg gtctggcgtt gctttcctct ttcttatgca tgtttattgc
    13621 cagcggttcc gcaatctggt cgattctggc ccccattttc gtaccaatgt ttatgctact
    13681 tggctttcac ccggcatttg cgcaaatcct ctttcgtatt gccgactcat ccgtattgcc
    13741 tttagcgccg gtatctcctt ttgttccact gtttcttgga ttcctgcaac gctacaaacc
    13801 agacgcgaaa ctgggtactt actattcgtt agtcttgccc tatccgctta tctttttggt
    13861 ggtatggctg ctgatgttgc tggcgtggta tcttgttggt ctgccgatag gtccggggat
    13921 ttacccacgt ttgtcttaag agagaacgga tgctgagatt acttgaagaa aaaattgcca
    13981 cgccactggg tccactgtgg gtgatttgcg atgagcaatt tcgcctgcgg gcggttgaat
    14041 gggaagagta cagcgaacgc atggtgcagc tactggacat ccattatcgc aaagaaggct
    14101 atgagcgcat ttctgccacc aacccaggcg gtttaagcga caagcttcgt gattattttg
    14161 ccggtaatct tagcattatt gatacgcttc ccactgccac aggggggacg ccatttcagc
    14221 gcgaagtctg gaaaactcta cgcactatcc cctgcgggca ggtaatgcat tacggccaac
    14281 tggctgaaca attgggccgc cctggcgcgg cgcgtgccgt tggtgcggca aacggatcga
    14341 atcccatcag catcgtcgta ccttgccatc gggttattgg ccgaaacggc accatgaccg
    14401 gatatgcagg cggagttcag cgaaaagagt ggttattgcg ccacgaaggt tatcttttac
    14461 tgtaaacatt aaacaatttg tgccagcttg ttcacacttt tatgtaaagt tacccttaac
    14521 aacttaaggg ttttcaaata gatagacata tatttacatc taatatcgga attctctgct
    14581 gttaaggttt gcttagactt acttgctccc taaaaagatg ttaaaattga caaatatcaa
    14641 ttacggcttg agcagaccta tgatcccgga aaagcgaatt atacggcgca ttcagtctgg
    14701 cggttgtgct atccattgcc aggattgcag catcagccag ctttgcatcc cgttcacact
    14761 caacgaacat gagcttgatc agcttgataa tatcattgag cggaagaagc ctattcagaa
    14821 aggccagacg ctgtttaagg ctggtgatga acttaaatcg ctttatgcca tccgctccgg
    14881 tacgattaaa agttatacca tcactgagca aggcgacgag caaatcactg gtttccattt
    14941 agcaggcgac ctggtgggat ttgacgccat cggcagcggc catcacccga gcttcgcgca
    15001 ggcgctggaa acctcgatgg tatgtgaaat cccgttcgaa acgctggacg atttgtctgg
    15061 taaaatgccg aatctgcgtc agcagatgat gcgtctgatg agcggtgaaa tcaaaggcga
    15121 tcaggacatg atcctgctgt tgtcgaagaa aaatgccgag gaacgtctgg ctgcattcat
    15181 ctacaacctg tcccgtcgtt ttgcccaacg cggcttctcc cctcgtgaat tccgcctgac
    15241 gatgactcgt ggcgatatcg gtaactatct gggcctgacg gtagaaacca tcagccgtct
    15301 gctgggtcgc ttccagaaaa gcggtatgct ggcagtcaaa ggtaaataca tcaccatcga
    15361 aaataacgat gcgctggccc agcttgctgg tcatacgcgt aacgttgcct gatttttccg
    15421 cataactcac tatccttctg tcatatcatt aaatttttct gatttattga tctggcagaa
    15481 ggttcatcac tgtttcattc accagatatg ggttaatctt ttaattacaa actgcgttga
    15541 cagttgttgt aaggagaccc tgtatggcta tgtatcagaa catgctcgtt gttatcgatc
    15601 ctaaccagga cgaccaacca gcattgcggc gagctgttta tttacatcaa cggattggtg
    15661 gcaaaattaa agcctttttg ccgatctatg acttctcata cgaaatgacc accctgctct
    15721 ccccggacga acgtaccgct atgcgtcagg gcgtcatcag ccagcgtaca gcctggatcc
    15781 acgagcaggc aaaatattat ctcaatgctg gcgttcccat tgaaattaaa gtggtctggc
    15841 ataaccgtcc cttcgaagcc atcattcagg aagtgatcag cggcgggcac gatttggtgc
    15901 taaaaatggc gcaccaacat gaccgtctgg aagcggtgat ttttacgcca acggactggc
    15961 atctgttacg caaatgccca agcccggtgt ggatggtgaa agaccagccg tggccggaag
    16021 gaggcaaggc gctggtggcg gtgaatctcg ccagtgaaga gccgtatcat aatgcgctca
    16081 atgaaaaact ggtcaaagag acgatcgaac tggcagaaca agtcaaccat accgaagttc
    16141 atctggttgg cgcttatccg gtaacgccaa ttaatatcgc gattgaactg ccggaatttg
    16201 atccgagcgt ttataacgat gccattcgtg ggcaacattt gctggcaatg aaagctctgc
    16261 ggcagaaatt cggcattaat gaaaacatga cgcacgtaga aaaaggtctg ccagaagagg
    16321 tgattcctga tttggcggag cacttacagg cggggattgt ggttctgggc acggtaggac
    16381 gcaccggtat ttcagcagca ttcctcggca acacggcgga acaggtgatt gatcatcttc
    16441 gctgcgatct gttggtcatt aaacctgacc agtatcagac acccgttgaa ctggatgacg
    16501 aagaagacga ttaacgactc ttcaataact gacaaggccc gagttatcgg gcctattttt
    16561 tacttactgc tcttcgccgc caaggaaata aattcccaac gggattgcca gcaacacagt
    16621 aaagaccagg ctataaacaa aaatcatcga tgactgaatc acatacattg atgtcgtcca
    16681 ctcagacagc ggaatattgt actgttctat aacgccgccg atagtcgcgc gagtcacaac
    16741 cgacgccgca ataatgaaaa ccgccaacaa accaaagaga aatttcttcc ctttcgctga
    16801 cttcagtttc gccataatca tgatgtaatc cttgtgcaga caaatttctt gttacctaaa
    16861 cgtaacaata ccatgacatt tcaccgttgt ctgctctgaa aacctcacct cttagctgga
    16921 tttacaatca acgcattgat tagttagcta atgttgctac ttccttccct gactattgat
    16981 gatctgaatg aattgtcgcg gtaaaatagc cccgccaggg gtttccggca tgacgacaat
    17041 tatctggagg agcctgataa aacaatgatc gctgaactgt ttacaaataa tgcgcttaat
    17101 ctggtcatta ttttcggtag ctgcgcagca ttgattctga tgagcttttg gtttcgccgc
    17161 ggaaatcgta aaagaaaagg ttttttattc catgcggtgc aatttttaat ctacaccata
    17221 attatcagtg ctgttggtag cattattaat tatgttatag aaaactataa actcaaattt
    17281 atcaccccag gcgttatcga ttttatctgt acgtccctga ttgcggttat tctgacgatt
    17341 aagctatttt tgctgattaa tcagtttgaa aaacagcaga ttaaaaaagg tcgcgatatc
    17401 accagtgcgc ggattatgtc gcgcattatc aaaatcacca ttattgtggt gcttgttctg
    17461 ctttatggcg aacatttcgg catgagcctt tctggcttgc tgacctttgg tggtattggt
    17521 ggtctggctg tcggtatggc cggtaaagat attctgagta atttcttttc cgggatcatg
    17581 ctctatttcg accgtccttt cagtattggc gactggatcc gttcaccgga cagaaatatc
    17641 gaaggtacag tagcggaaat tggctggcga attaccaaaa ttacgacctt tgataatcgt
    17701 ccattgtacg taccgaactc gctgttttcg tcgatcagcg tagaaaaccc aggacgaatg
    17761 accaaccgcc gcattaccac gaccattggt ttacgttatg aggatgcggc aaaagtgggc
    17821 gttattgtcg aagccgtacg tgagatgctg aaaaatcacc cggccatcga ccagcgacaa
    17881 accttactgg tttatttcaa ccagtttgcc gactcgtcat tgaatattat ggtttattgc
    17941 tttaccaaaa ccacggtatg ggctgaatgg cttgctgcac agcaagacgt ttatttgaag
    18001 attatcgata ttgtgcagtc acacggcgcg gactttgcct tccccagcca gacgctgtat
    18061 atggataaca ttacaccgca ggaacagggg cgctaaatca gacatcactg gcgttatcca
    18121 caagggtaac gccagttccc atcacatcaa tgcttcacaa tatacatagt ccggctgtac
    18181 gccacatctt ccgggttatt aatgggataa cctttcagcc acggcttgat taatcgtcca
    18241 ttggtatatt gataaattgg tgcaatcggt gcttgctcca tgaggatttt ttctgccgcg
    18301 ttgtaatcgg cattacgcgc tttaacggta ttttccgtcg atgcctgggc cagaactttg
    18361 tcgtatgccg gattgttaaa gcgtgaaata tttcctgaat gcgttgacgt taataatgtc
    18421 aggaaagtgg agggttcatt ataatccccc acccatgagg cgcggataac atcaaaattg
    18481 ccggtgttac ggctatcgat ataggttttc cattcctgat tttgcaattt aacatctacg
    18541 ccaaggttct ttttccacat cgatgctaca gcaatcgcaa ttttttgatg gttttctgaa
    18601 gtgttataca aaagcgtcag cttcagcggt ttttgcggac cataaccagc tgcgctcaac
    18661 aaagtttttg cctgcgcatt cagttcttcc tgactcattt gttcaaacgg cgaaggttcc
    18721 ggcgtaaatc cggcggtaac atctggtgta aaatgccacg ctggcttttc gcccgtccct
    18781 aatacttttt cggtcatcag gcggcgatct atcgtcatac ttaatgccag acgaacgcgc
    18841 tgatctgccg tcggcccttt ttgcgtgtta aacgcataat aataggtccc gagctgcggc
    18901 ggcgtataaa cctgccccgg aatatccttc aacagcttct gatacatatt tttcgggaag
    18961 gattcggtga tatcaatatc ccccgcgagg taacgcttag tggctgcgga ttcctgatta
    19021 attggcagga aggtcacttt ttgcagtacc gttttggcgt tatcccaata atgggtattc
    19081 ggtaccacga ccagtttttc attgactacg cgttctttaa gaacataagc gccattgccg
    19141 atcagatttc cgggtttcgt ccactcttta ccgctttcta cgttggcttt ttgcaccggg
    19201 aagaaggcaa agttagcggt taaattcaca aaccacggca acggtttatc aagctgaatt
    19261 ttcaaggtat gggcatcaac tgcggtgacg ccaagctggt caggcgtagc tttaccatca
    19321 ataatcgcct gtgcgttgtt gattcccgcc agcgcggcaa accatgcaaa tggcgatagc
    19381 gttttcgggt ccaccagacg ttgccagctg tagacaaaat cttgcgccgt taccggtgtg
    19441 ccatccgccc attttgcgtt atcgcgcagg gtaaaagtcc agatacggtt gtcattactt
    19501 ttccactgag tcgcaacgcc ggggacaatc tcccctttct cgttctgatt caccagtcct
    19561 tcaaacagat cgcgaatgac ctgaatctct ggcaggccca cggctttagc gggatccagc
    19621 gacgcaggct catctttaat gtggcgcacc agctcctgct tctctgccag tactgtgccg
    19681 ctcggaactt ctgcagcata cgaaagagaa atgctgctga ccaacagcgc acaacacgtg
    19741 actgaaacag agtgcttcat aagtttccct ctaaccgaca ttaaaattga tgcgtaatta
    19801 tttgtttcgc cttaaagaaa tgcaaacaac ttccatcaaa aaagtgacac aggaagcata
    19861 aaatgtttta tgtatcaata tattaaatca gatttagtgt aaataatcga cgccaaaatg
    19921 cggcaaagca catagaacac cgaacactac gttcaggctt cgcacgttca aaccagtttt
    19981 cggtatcaaa atcagcatct gtcgccattg tacgaagaat aaaaaataac caaaacgttg
    20041 aaaggaactg agaatacgtt aagagagaaa ttaaattaat gtatcagcaa gtagtgctaa
    20101 acagcacctt tatacttcag cctatctata aataatgaga atgccgagcc agcatgtcga
    20161 cagtgtggat agtacaggtg ataaccgggt aaatctggtg tgaatttatc caaaacacgt
    20221 atcagttttt tttctttaat agcccgctca acctgatcat aaggtagata cgccaatcca
    20281 tgcccatcaa ttgcagcatc aatgatcagg tctatcgtat tcagtaaaag ctgaccttcc
    20341 atgcgaacac gaacttcacg tccaccgcgt attaatctcc agcgatttgc tgtacccgat
    20401 gtgggaagat acaaattaat tgcctgatga tctattaatt gtgacactga cgttggaaca
    20461 cttcggcgag aaaaataatc cggtgagcca acaatagcca ttggtatatc tggcccgatt
    20521 cgaatggcga tcatatcttt atccatctcc ccacccagac ggacgcctgc atcaaaacgt
    20581 tcagaaacga catcggtcaa accataatca atggtgagct gaatatcaat ttcaggatgc
    20641 gatttcagga atgtgcgcat tgctggtaac aatatcgttt ttgctgcatg ttctacagta
    20701 gtaatacgta ttgtcccgga tgggcggttc tgcagatcgc tcagggatgc catggctgaa
    20761 tctatgtcat gcaacatagg gccaagaaca gacaaaagat gctcgcccgc ttcagttgga
    20821 acaacgctgc gcgtggttcg cgtcagaagc cgcaatccca atcgttcttc tatacgacgc
    20881 actatctggc ttaaagctga ctgcgccatg ctcaggcggg ctgctgcacg agtgaagcta
    20941 cgctcctctg caacgacgac aaacgccatc agatcagcga tttcttcacg cttcattcag
    21001 tatctccatg caatctatta atcatttaaa tgataggtct aatcatgatt ttgggtctaa
    21061 tcaacttaat gatcaatatc tatagtgata tcaccaaccg ttaaaacgaa ccgacaactc
    21121 catgaggaac ggaatgatga ataataaagt cagcttcact aacagcaata atccaaccat
    21181 ctctttgtct gcagtgatct atttccctcc aaaatttgat gaaacccgtc agtatcaggc
    21241 aattgtgtta tctcacccag gtgggggcgt taaagaacaa acggccggaa cctatgccaa
    21301 aaaactggcg gaaaagggat ttgtcactat tgcttatgac gcatcttatc aaggtgaaag
    21361 tggcggcgag ccgcgtcagt tagaaaaccc atacattcgt actgaagaca tcagtgccgt
    21421 gattgattac ctgacaacgc tttcttatgt cgacaatacc cgaattggtg cgatgggaat
    21481 ttgtgccggt gcaggatata cagccaatgc tgcaattcag gatcgccgca tcaaggccat
    21541 cggtaccgtt agtgcggtca atattggttc gatgttccgt aatggttggg aaaacaatgt
    21601 gaaatctatt gatgccctgc catacgttga agcgggttca aacgcccgaa ccagcgatat
    21661 tagcagcgga gaatatgccg ttatgccact ggcaccaatg aaggagtccg atgcgccgaa
    21721 cgaagaactg cgtcaggcct gggagtatta ccacacccct cgcgcgcagt atccaacagc
    21781 accgggttac gctactttgc gcagccttaa ccagattatt acctatgatg cttaccatat
    21841 ggcggaagtg tacctgactc agccgatgca gattgtggcg ggcagccagg cagggagcaa
    21901 atggatgagt gacgacctgt acgatcgggc atcaagccag gataaacgct atcacattgt
    21961 tgaaggcgca aaccatatgg atctttatga cggcaaagcc tatgtggctg aagcaatttc
    22021 agtattagct ccgttctttg aggaaactct gtaatgaaac atattcaaat ccgcaatagt
    22081 gatatggcct ggcatattgc tgccaatatt cagtttccac caaattttga tgagtcaaaa
    22141 cagtatccgg cgattatcag cgtccatcct tttggcagtt gtaaggagca gacgtctggc
    22201 aatatctatg gcaaagcatt ggctgaaaaa ggctatctcg ttctcgctta tgacgccagt
    22261 ttccagggag aatctggtgg ggagcctcgc tggatagaag atccgacaca gcgcgtagag
    22321 gatattagcc gtgttatcga ttatgcagta actctgccat atgttaatgc tgaacgtatt
    22381 ggcgtgctgg gagtgtgtgg gggtggtggc tatgccatta atgccgcgct gacagaaaaa
    22441 cgaattaaag cagtagtgag tattacgggt gttaatatcg ggcgtctttt tcgggaaggt
    22501 ttcagcaatt acgatccgat tggcgcattg aatgcaatgg cgtcgcaacg aaccaaagag
    22561 gctcgtggtg gagaattaca gatcaacgaa ttacttcccg ccagcctaga tgcagcaaaa
    22621 gcccatggac tgaccgaacg cgatgtttat gaagcaactg actactataa aacaccacgc
    22681 gggcaacaac cgggaggtgc gacaaaaatg ttgttctcac atgcacagaa aacgctggcc
    22741 tgggatgcct tcgcatttac tgaagtgtta ctgactcagc cggtgatggt agtagttggt
    22801 gaaaaagtcg gtgccttcgg tgcttatcgt gatggactgg aagtctatgg ccgggcgatg
    22861 gtttcgcagg atcgtcagtt agtttcatta cccgatttct cacattatga actgtatgac
    22921 aaaccggaag cagttcagga ggcgctggaa aaagttattc cttttttcaa cacccatctg
    22981 ggataagttt catgagaaat attatgaaaa atgtactgat tcttggtgct ggcggtcaga
    23041 tagcccgcca tgtgattaat caactggcag ataaacaaac cattaaacaa actttatttg
    23101 cccgtcagcc agcaaaaatc cacaaaccct atccaactaa tagcaagatt attatgggag
    23161 atgtactgaa tcatgcagca ctgaagcagg ccatgcaggg acaggacgtt gtgtatgcca
    23221 accttacggg ggaagattta gatattcagg caaattcagt gatcgctgcg atgaaagcct
    23281 gtgatgttaa acgtctgatc tttgttcttt ccctcggtat ttatgatgaa gttccaggaa
    23341 aatttggcga atggaataat gcagttattg gtgagcccct aaaaccattc cgcagggctg
    23401 cggatgcaat tgaggcttct ggactggaat atacaattct tcgccctgcc tggctgactg
    23461 atgaagatat tattgattat gagcttacct cacgcaatga accattcaaa ggtacgatcg
    23521 tatcacgtaa gagtgtcgca gcattaatca cagatatcat cgataaacct gagaaacaca
    23581 ttggtgataa tatag
//
LOCUS       DAZGAN020000069        23447 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGAN020000069 DAZGAN020000000
VERSION     DAZGAN020000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.069"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            45..1094
                     /gene="yeiH"
                     /locus_tag="ACHKYO_RS15195"
                     /old_locus_tag="pgaptmp_003039"
     CDS             45..1094
                     /gene="yeiH"
                     /locus_tag="ACHKYO_RS15195"
                     /old_locus_tag="pgaptmp_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGU4429852.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1168..2025
                     /gene="nfo"
                     /locus_tag="ACHKYO_RS15200"
                     /old_locus_tag="pgaptmp_003040"
     CDS             1168..2025
                     /gene="nfo"
                     /locus_tag="ACHKYO_RS15200"
                     /old_locus_tag="pgaptmp_003040"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HGU4429853.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2029..3117
                     /locus_tag="ACHKYO_RS15205"
                     /old_locus_tag="pgaptmp_003041"
     CDS             2029..3117
                     /locus_tag="ACHKYO_RS15205"
                     /old_locus_tag="pgaptmp_003041"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU4429854.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3173..4423)
                     /gene="nupX"
                     /locus_tag="ACHKYO_RS15210"
                     /old_locus_tag="pgaptmp_003042"
     CDS             complement(3173..4423)
                     /gene="nupX"
                     /locus_tag="ACHKYO_RS15210"
                     /old_locus_tag="pgaptmp_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU4429855.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGSLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4523..5464)
                     /gene="rihB"
                     /locus_tag="ACHKYO_RS15215"
                     /old_locus_tag="pgaptmp_003043"
     CDS             complement(4523..5464)
                     /gene="rihB"
                     /locus_tag="ACHKYO_RS15215"
                     /old_locus_tag="pgaptmp_003043"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HGU4429856.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5594..6292
                     /gene="yeiL"
                     /locus_tag="ACHKYO_RS15220"
                     /old_locus_tag="pgaptmp_003044"
     CDS             5594..6292
                     /gene="yeiL"
                     /locus_tag="ACHKYO_RS15220"
                     /old_locus_tag="pgaptmp_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HGU4429857.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLTKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6363..7613)
                     /gene="psuT"
                     /locus_tag="ACHKYO_RS15225"
                     /old_locus_tag="pgaptmp_003045"
     CDS             complement(6363..7613)
                     /gene="psuT"
                     /locus_tag="ACHKYO_RS15225"
                     /old_locus_tag="pgaptmp_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU4429858.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7707..8645)
                     /gene="psuG"
                     /locus_tag="ACHKYO_RS15230"
                     /old_locus_tag="pgaptmp_003046"
     CDS             complement(7707..8645)
                     /gene="psuG"
                     /locus_tag="ACHKYO_RS15230"
                     /old_locus_tag="pgaptmp_003046"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HGU4429859.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(8633..9574)
                     /gene="psuK"
                     /locus_tag="ACHKYO_RS15235"
                     /old_locus_tag="pgaptmp_003047"
     CDS             complement(8633..9574)
                     /gene="psuK"
                     /locus_tag="ACHKYO_RS15235"
                     /old_locus_tag="pgaptmp_003047"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HGU4429860.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(10031..11722)
                     /gene="fruA"
                     /locus_tag="ACHKYO_RS15240"
                     /old_locus_tag="pgaptmp_003048"
     CDS             complement(10031..11722)
                     /gene="fruA"
                     /locus_tag="ACHKYO_RS15240"
                     /old_locus_tag="pgaptmp_003048"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HGU4429861.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWQGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVDASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(11739..12677)
                     /gene="fruK"
                     /locus_tag="ACHKYO_RS15245"
                     /old_locus_tag="pgaptmp_003049"
     CDS             complement(11739..12677)
                     /gene="fruK"
                     /locus_tag="ACHKYO_RS15245"
                     /old_locus_tag="pgaptmp_003049"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGU4429862.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12677..13807)
                     /gene="fruB"
                     /locus_tag="ACHKYO_RS15250"
                     /old_locus_tag="pgaptmp_003050"
     CDS             complement(12677..13807)
                     /gene="fruB"
                     /locus_tag="ACHKYO_RS15250"
                     /old_locus_tag="pgaptmp_003050"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HGU4429863.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            14175..15356
                     /gene="setB"
                     /locus_tag="ACHKYO_RS15255"
                     /old_locus_tag="pgaptmp_003051"
     CDS             14175..15356
                     /gene="setB"
                     /locus_tag="ACHKYO_RS15255"
                     /old_locus_tag="pgaptmp_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HGU4429864.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(15353..15607)
                     /gene="yeiW"
                     /locus_tag="ACHKYO_RS15260"
                     /old_locus_tag="pgaptmp_003052"
     CDS             complement(15353..15607)
                     /gene="yeiW"
                     /locus_tag="ACHKYO_RS15260"
                     /old_locus_tag="pgaptmp_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU4429865.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15762..16334
                     /gene="yeiP"
                     /locus_tag="ACHKYO_RS15265"
                     /old_locus_tag="pgaptmp_003053"
     CDS             15762..16334
                     /gene="yeiP"
                     /locus_tag="ACHKYO_RS15265"
                     /old_locus_tag="pgaptmp_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HGU4429866.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16557..18023
                     /gene="yeiQ"
                     /locus_tag="ACHKYO_RS15270"
                     /old_locus_tag="pgaptmp_003054"
     CDS             16557..18023
                     /gene="yeiQ"
                     /locus_tag="ACHKYO_RS15270"
                     /old_locus_tag="pgaptmp_003054"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU4429867.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGCE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            18141..19127
                     /gene="yeiR"
                     /locus_tag="ACHKYO_RS15275"
                     /old_locus_tag="pgaptmp_003055"
     CDS             18141..19127
                     /gene="yeiR"
                     /locus_tag="ACHKYO_RS15275"
                     /old_locus_tag="pgaptmp_003055"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HGU4429868.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSTLLKLRLATTA"
     gene            19166..19879
                     /gene="lpxT"
                     /locus_tag="ACHKYO_RS15280"
                     /old_locus_tag="pgaptmp_003056"
     CDS             19166..19879
                     /gene="lpxT"
                     /locus_tag="ACHKYO_RS15280"
                     /old_locus_tag="pgaptmp_003056"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HGU4429869.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVTGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            20291..20857
                     /gene="mepS"
                     /locus_tag="ACHKYO_RS15285"
                     /old_locus_tag="pgaptmp_003057"
     CDS             20291..20857
                     /gene="mepS"
                     /locus_tag="ACHKYO_RS15285"
                     /old_locus_tag="pgaptmp_003057"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HGU4429870.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            21038..22594
                     /gene="pdeN"
                     /locus_tag="ACHKYO_RS15290"
                     /old_locus_tag="pgaptmp_003058"
     CDS             21038..22594
                     /gene="pdeN"
                     /locus_tag="ACHKYO_RS15290"
                     /old_locus_tag="pgaptmp_003058"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGU4429871.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTTIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            22676..>23447
                     /locus_tag="ACHKYO_RS15295"
                     /old_locus_tag="pgaptmp_003059"
     CDS             22676..>23447
                     /locus_tag="ACHKYO_RS15295"
                     /old_locus_tag="pgaptmp_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4429872.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDD"
BASE COUNT         5810 a         6086 c         5648 g         5903 t
ORIGIN      
        1 aaaccagtta gtcgtaacct tatgcccggt taaaggagag ggttatgacg aatatcacct
       61 tacagaaaca acatcgtaca ctgtggcatt ttattccggg gttagccctg agtgcagtta
      121 tcaccggggt cgccctgtgg ggtggttcca ttcccgcggt tgcgggtgcc gggtttagtg
      181 ccctcaccct cgcaatcttg ttggggatgg ttttaggcaa caccatctat ccgcacatct
      241 ggaaaagctg tgacggtggt gtgctgtttg ccaagcaata tttattacgt ctgggtatca
      301 ttctttatgg cttccgtctg acgttctcgc aaattgccga tgtcggtatc agtgggatca
      361 tcattgacgt gttgacgctg tccagtacct tcctgctggc ttgcttcctg gggcagaaag
      421 tgtttggtct ggataagcac accagctggt tgatcggtgc cggtagcagt atctgtggtg
      481 ctgccgcggt actggcgact gagccggtag tgaaagcgga agccagtaaa gtaaccgtgg
      541 ctgttgcaac cgttgttatc ttcgggaccg tcgcgatttt cctctacccg gcgatatatc
      601 cgctgatgtc tcaatggttt agtccggaaa ctttcggtat ctacattggt tctactgtgc
      661 acgaagtggc gcaggtggtg gcggcaggtc atgccatcag cccggatgcg gaaaacgcag
      721 cagttatttc caaaatgctg cgcgtgatga tgctggctcc gttcctcatc ctgctggcgg
      781 cgcgtgttaa acagctgtct ggggcgaaca gcggcgagaa aagcaaaatc actattccgt
      841 ggtttgccat cttgttcatc gtagttgcca tctttaactc gttccacctg ttaccgcaga
      901 gcgtggtgaa catgctggta acgctggata ccttcctgct ggcaatggcg atggcggcgc
      961 tgggtctgac cactcacgtc agcgcgctga aaaaagccgg ggcgaaacct ctgctgatgg
     1021 cactggtact gtttgcctgg ctgattgttg gtggtggtgc gattaactat gtgattcaaa
     1081 gcgtcatcgc ataaaccact acatcttgct cctgttaacc cgctatcatt accgttttcc
     1141 tccagcgggt ttaacaggag tcctcgcatg aaatacattg gagcgcacgt tagtgctgct
     1201 ggcggtctgg caaatgccgc aattcgcgcc gccgaaatcg acgcaaccgc gtttgccttg
     1261 ttcaccaaaa accaacgtca gtggcgtgcc gcaccgctca cgacgcaaac catcgatgaa
     1321 ttcaaagcgg cctgtgaaaa atatcactac acatcggcgc aaattcttcc ccacgacagc
     1381 tatctgatta acctcggaca tccggtcact gaagctctgg aaaaatcgcg cgatgccttt
     1441 atagatgaaa tgcagcgttg cgaacaactg gggctttctt tgctcaactt ccaccctggc
     1501 agccatctga tgcagatttc agaagaggat tgccttgcgc gtattgccga atccatcaac
     1561 attgcgctgg ataaaactca aggtgtaaca gcggtgattg aaaacaccgc cggtcagggc
     1621 agtaacttag ggtttaaatt cgaacatctc gcggcgatta tcgacggcgt ggaagataaa
     1681 tcccgtgtcg gcgtctgcat tgatacctgc catgctttcg ctgccgggta tgatttgcgt
     1741 actccagccg aatgcgaaaa aacgttcgct gattttgccc gtattgtcgg ctttaagtat
     1801 ctgcgcggga tgcaccttaa cgatgcgaaa agtacctttg gcagccgcgt tgaccgccat
     1861 catagcctcg gtgaaggcaa tatcggtcat gatgcgttcc gctggatcat gcaagacgac
     1921 cgtttcgacg gcattccgct gatccttgaa accatcaacc cggatatctg ggcagaagag
     1981 atcgcctggc tgaaagcgca acaaactgaa aaagcggtgg cctgatcgat gaacgacagg
     2041 gaaaagcaga ttcttaaaat tttacggcgt aacccgctga ttcaacagaa tgaaatcgcc
     2101 gatattctgc aaatcagccg ctcacgtgtt gccgctcata ttatggattt gatgcgcaag
     2161 ggtttgatta aaggcaaagg atatatcctt accgagcagg attactgtgt ggttgtgggg
     2221 gcaatcaata tggatattcg cggaatggcg gatatccgtt acccgcaagc agcctctcat
     2281 ccaggttctg ttcactgttc cgcaggcggc gtcggacgta atatcgcgca taacctggct
     2341 ctgcttggtc gggatgtgca tttaatatca gctattggca acgattttta cggcgagaca
     2401 ttactggaag aaacgcgccg cgcgggcgtg aacgtttcaa actgcattcg tctgcatgga
     2461 cacagcactg caacttatct cgctatcgct aataagcagg aagaaactat cctggcaatc
     2521 aatgacacac atattctgca acagctaacg cctcaactgc tcaatacttc acgggacttg
     2581 attcgccatg ctggtgtggt gctggctgat tgtaatctca cacctgaagc cctggagtgg
     2641 gtctttacca ttgctgatga aattccgatg tttgtcgata ccgtttccga atttaaagca
     2701 aacaaagtaa aaaactggta cagccggatc cacactctga aaccaacaca aaatgaactg
     2761 gaaattttgt gggggcaacc gattaaagac gacaacgatc gcatcagagc tgtaaattcg
     2821 ctgcatcaac aaggtgttaa acgcattttt gtttatctga aagatgaatc tgtattttgc
     2881 agcgataagg atggagaaca atttttgctg accgcgccag cgcatacgac agtagacagt
     2941 tttggtgctg acgatggttt tatggcgggc ctggtatata gctttcttga aggatacagt
     3001 ttccgcgaca gcgcccgttt tgcggtagcc tgcgcggcaa tttcgcgcgc cagcggcagc
     3061 ttaaacaacc ctaccctgtc tgccgataac gcgctttcat tagtgccaat ggtgtaacaa
     3121 tgttgccgga ttcgacgctt gaccgcatct tatccggctc ataaattgcg cactacgcta
     3181 aaccaataaa gaatcctgca atagtagcac tcatcaggtt agaaagtgtc gccgccgcca
     3241 gcgcgcgtaa accaagttgg gcgatttccg gcgcacggtg tggcgcaacc gcagaaaacg
     3301 cccccaccac caccccgata gaaccaaagt tagcgaaacc gcacaacgcg aaagaaataa
     3361 tcgccacggt tttagcatcc agagagccac ccgtttgcag atatggcgag aaattgagat
     3421 aagcgacaaa ttcattgatc gccagcttct gcccaatcaa actcccggca agatttgcat
     3481 cactccagtc aacccccatc acccacgcca atggggccaa caggtaacct aaaatggact
     3541 ccagcgaggc atgtgcaaaa ccaaaccagc cgccaacgcc gccgataata ccattaatta
     3601 acgcaatgat ggcgacaaat gccataacaa ctgtcgctac accggcggcg attttcagcc
     3661 cggtcattgc ccctgtcgca gcggcttcaa taatgctttt tggcggtgtt tcggtgaaag
     3721 agaggttatt aaaggaaacc tgcgaagatt ccgtagccgg gcttaacagg cgggcaaaca
     3781 agatcccgcc tgggatcgcc attaacgatg ccgccagcaa atattccaca ggtacgccca
     3841 gggcggcgta accaatcatt gtcgaaccag caatcgaggc catgccacta caaatcgctg
     3901 taaataattc attgcgattc agacgatcga taaagggttt gacgatcgcc ggaatttcgt
     3961 tttgcccgag gaaaatggtg gtgaccgcga cgaatgactc aatcttgctg atatttaatg
     4021 ctttctggaa tataccgccg agaatgcgaa ttaaaatccc catcacaccg atgtagtaga
     4081 gaatactcac cagtgcagtg acgaagataa ttgccggtaa taccctgaaa ccaaagataa
     4141 atcctgcgcc atcaaacagc gtgtccattt tcggcccgac cagcgaaccg aagataaatg
     4201 cgctacccgc gtcgctgtac gccatcactt tatgcacgcc aaaagcgact ttttcagcga
     4261 cccaacgccc tggcggtaac caaagcataa tcccgccaat cacgacctgt aacactaacg
     4321 ccgcgccaac ggtacgcagg ctgatcttct tcttgtttac tgacagcaaa aacgcaatcg
     4381 tcagcaatac caccattccc agaacacttc tcatgacatc catagttatt atctcttcat
     4441 gtcatattgg gcgacggctt tctgcccatc agaaatagcc gggcttggat cttgcgatac
     4501 tgacccggct tgttatcaac acttaatggg ttttgatgta gccgcgcacg cactcttcga
     4561 ctaagcccca gaaccagtcg gtatcaatag tgatgccgac tttggtatta gcgggcttgc
     4621 caagaacgcc cagctcgtcg cagacggtac gcccatagca agggccactg ttgacgtcca
     4681 cttcgacgta catctcctgt gttttaatgc catcagggtt aatcagataa ccgatgcagg
     4741 tggcgtcgtg caccgggccg ccagccaggc cgtagttttc gaactgcgtt ttgagagtga
     4801 agttcatgat gtcgctgaac agctctccgg cagggccgcc tgccctttcc atccgtgcaa
     4861 tcacgtccgg ggtgcaaacg gtctggttgg tgagatcgag gcccatcatc actaatggaa
     4921 cgccggaggt gaacaccacg cgggctgctt ccggatcagc aaagatgttg aactcggcag
     4981 atggcgtgaa gttgcctgta ccgtaagcgc cgcccattag cacaatttca cggattttgg
     5041 gcaggatcgc gggttgcata cgcattgcca ccgcgatatt tgaaagcgga ccaaccggca
     5101 ccagagtgat atcgccatcg cttgccatca gggtatcaat gatgtatttc accgcgtggg
     5161 tactttctgc ctggcgggtc agcggctcga ataccgggcc atccagtccg gtttcaccgt
     5221 gaatattatc ggcaacgatt tgtttacgca taatgggttg cggcatcccc gcataaaccg
     5281 gaacattaat ctccagtttc tggcaaacat tcaggccatt aattaatgtt ttatcaagcg
     5341 tctgattacc cgctacaata gtgatgccta ataaatctat tgccggatgt ttcgctgcca
     5401 tcatcattgc aatagcatca tcatgacccg gatcacaatc cagaataatt tttctctttt
     5461 ccattgttta tttcctctgt ttccagttgc gttatttttt ctacagcaaa gaaaattcga
     5521 ccgggccgat gattgaatct taccaaccgc gtacgtatgc taaatatgag aaatctcata
     5581 gcggataaac atcgtgaaag aaatccacaa taatgatctt aagcagcaat tgatgagtga
     5641 atccgcgttt aaggattgct ttttaacgga tgtttcagcc gatacgcggc tgtttcattt
     5701 tttagcgcgt gactacattg tgcaggaagg gcaacagccg tcctggctgt tttacctgac
     5761 gcgaggccgc gccaggcttt acgccacgct agctaatggt cgcgtgtcgc tgatcgattt
     5821 ctttgccgcc ccctgtttta ttggcgagat tgagttaatc gacaaagacc atgaaccgcg
     5881 tgcggtgcag gctattgaag agtgttggtg ccttgcgctc cctatgaaac attaccgccc
     5941 gctgttatta aacgacacgc tatttttacg aaaactctgc gtcaccttaa gtcataaaaa
     6001 ttatcgtaat attgtttctt taactcagaa tcaatcattt ccgttagtta atcgcctggc
     6061 agcttttata ttactctcgc aggaaggtga tctttatcac gaaaagcata cgcaagcggc
     6121 agagtattta ggcgtttctt atcgacatct tttatatgtt ctcgcgcaat tcattcacga
     6181 cggtttatta actaaaagca agaaagggta tctcattaaa aacagaaagc agttgtcagg
     6241 actggcgctg gagatggacc cggagaataa attctccggg atgatgcagt aaaaattatt
     6301 tgcaatagcg cgattgccgg atgcaacgct taatacgttt tatccggtct acaaatcgag
     6361 cattacgcca gaccaataaa gaacccggca atagtcgcac tcatcaggtt ggaaagcgtt
     6421 gctgctgcca gcgcccgtaa accaagctgg gcgatttctg gagcacgttt tggcgaaata
     6481 gccgaaaatg cgccaacgac aacaccgata gaaccaaagt ttgcaaaccc acagagggcg
     6541 aacgagataa tggcaatggt cttgacatcc agcgtgccgc tcgtttgcag atagggcgag
     6601 aaattgagat aagcaacaaa ctcattaatc gccagtttct gcccaatcaa acttccggca
     6661 agatttgcat cactccagtc cacccccata atccatgcca acggggccag cacatagccg
     6721 aaaatgcttt ccagagagac attggcaaaa ccaaaccagc cgccaactcc accaataatg
     6781 ccgttgatca gcgcaataat tgccacaaat gccattacca ccgtcgccac accggcggcg
     6841 attttcagcc cggtcattgc accgttcgcc gcagcttcaa taatgctttt tggcggtgtt
     6901 tcgctaaacg acagattttc aaacgtaact tgcgaaggtt ccgtcgccgg actaagaata
     6961 cgtgcaaaca ggataccgcc aggaatagcc atcagcgaag cggctaacaa atagtcaatc
     7021 ggcacgccca ttccggcata gccaatcatc atcgaacccg caatagacgc catgccgcta
     7081 caaatagccg taaatagctc gttgcgattc atgcgatcaa taaaaggttt aacgatcgcc
     7141 gggatctcat tttgcccgag gaaaatagtg gtgactgcga caaaagattc gattttgctg
     7201 atgttgaggg ctttctgaaa aatactgcca agaatacgaa tcagcagccc cattacgcca
     7261 atgtagtaca gcaagctgat aagcgcggta acgaaaataa tcgccgggag tacgcgaaag
     7321 gcgaagataa agcccgcacc gtcaaacaga acatccattt tgggaccgac cagcgaacca
     7381 aaaataaagg cacttcctgc gtcactgtag gacatcactt tatggacgcc taatgccgcc
     7441 tgctctactg cccattttcc cgggggaaaa tagagcatga tgccaccaat ggcaatttgc
     7501 agcagcagcg cagcgccaac cgtgcgcaag ctgatgctct ttttatttac cgacaacaca
     7561 aaggcaattg ccagcaatac caccatcccc acaacacttc tcattatatc cataatgatt
     7621 ttcccttcat gccagtaaac ccggcgtcag cgccaggttt tggtatgttt gatgagtacg
     7681 ggcgacggct ttctgcccgc cagaaattaa cccacgagac gctgatattc tttggcaatt
     7741 tcgctcgcca gaatggcgtt attaaagacc agttgaatat tggatttcag gctatcaccg
     7801 cctgttagct ccgcaacacg cgccagcagg aacggtgtac tctctttccc aataacaccc
     7861 tgttcttcgg cttcagcaac ggcttgatca attgctgcat tgatggtttc ttctggcatc
     7921 gcaaactgtt ccgggatcgg attcgccact accaggccgc cgttcaaacc ggtttgccac
     7981 ttcactgcca tcgcgcgagc aatttcgcgg gcactgtcaa gacgaatgct gacttcaaat
     8041 gggctggttc ggcagaaaaa cgcaggcagc gcttttgtct gataaccaat taacggtaca
     8101 ccgaaggttt ctaaatattc tgttgttaat cctaaatcga gaatagattt tgcccccgca
     8161 caaacaacag tgacattggt atttgccagt tcctgtaaat cggcagaaat atcgaaggta
     8221 tgttccgccc cgcgatgcac accaccaatt cccccggtgg caaatacttt aattccggca
     8281 agcgccgcaa taatcatcgt tgaagccaca gtggttgcgc cattttttcc ggcggcaaca
     8341 acaaaaggta aatcgcgacg actaactttg gtcacgttat gtccttcacg gcccagcaat
     8401 tcgatttctt ctttacttaa gcccactttc atcacaccgc caataatggc gatagtggct
     8461 ggtactgcgc cctgtttacg aatagtttct tctacttcaa ttgcggtttg ggcattttgt
     8521 gggaatggca ttccgtgaga aattatggtc gattccagcg caacaaccgg ttttttattt
     8581 tttaaagcat cctgtacttc agcggatatt tgtaataatt caggggacag agttaattca
     8641 gacattctgt gttctccact aacgatctaa cattggcaat tgataattca gggttgttgg
     8701 tatattcaca ggcaagtgcc attgacgaac atccctgcgc aaatcgaaca gaatcgataa
     8761 acggcatacc gtctacccaa caggaggcca gccccgccat catggcgtcg cctgccccag
     8821 taacattgat gacattcgtt ttaatagggg ccgaccaccc actttcaccg ttgatatcac
     8881 tgtagtaaac gccatcccct cccatgctca gcactaggcg gttcaggccg tgttgatgaa
     8941 accaggcggc gacctttgcg acatcttcac gtccggaaag tgcaattccg ctcaacgttt
     9001 cggcttcaag acggttaggt tttaacgtat ggattttgct cagatgatcg cggattttga
     9061 cgcatttcca tgccgatacc ggatcaataa atacaggcgt ttccccgcta ttttccagaa
     9121 cccagaccag cgcctcttca ctaaggttgc aatccgcgac aataaccttt gcaccgcgaa
     9181 taaactcctg atgctgggca agaaattctg cagaaataca gtcactaata ctcatatcat
     9241 ttatcgcgac aagcatttct cctgtattat cgagcagtga taaatagctg gatgtattct
     9301 cacccggaac aattaagcat ttatcgacat aaacaccgga ttgattagtt tgcgccagta
     9361 atgattgccc ataaaaatca ccacctactg cacttaaaag ccaggcattt ttccccagta
     9421 acgccagatt atgagcgata ttccttccca cgccacctgg ggtaaattta atttttccgg
     9481 gattcgaatc cgcataattc aatggatgat gtgaataccc agccacatca atattcgccg
     9541 aaccaataat tactatgtag tccttttcgc gcatagcacg ccccctggca ataaattgcc
     9601 aacgaagaaa ttgggtaaaa tagtcattac agtcaataag actattagag cacgtgttca
     9661 tatttaaaca catgttcata ataagcatat ttaacgtaat gtaaatcgat cagaaaaaac
     9721 gaaatcggcc agatatgaag gcggtcacaa agtttgtcta tttatgcagc aacaaaagag
     9781 aaagttgtga tgtgtaacac acaatatggc aggcaagaaa aagggcagag atgtttctgc
     9841 cctccagatt gttgacaacg cgcgctttgt tcatcctgga tgtggcgtaa acgccttatc
     9901 cgacctacaa aagcgcaaaa attcaatata ttgcagggtt catgtaggtc tgataagcat
     9961 agagcatcag gcaattttgc gtttgtcatc atcggggcag agatgtctca tccccgataa
    10021 tgaagataaa ttatgcgttt ttttctacta tttgtgtttc cgggcgtttc aggaaggcat
    10081 aggccagacc cgccaccagc gtaccggcaa taatggctac caggtaaccc agtaccggcg
    10141 taatcgcgcc agggatcagc agaacaaaca gaccaccgtg cggtgccatc agtttcgcac
    10201 caatcgccat tgagattgcg ccagtcagcg ccccacccac gatacagcac ggcagcacac
    10261 gcatcggatc acgagcagca aacggaattg caccttccga aatgaagcac agtcccaata
    10321 ccagagcggc tttgccacct tcctgctgcg ctttgtcgaa tttgcgacgc gccaccattg
    10381 ttgccagacc cattgccagc ggtggcacca tacctgccgc cataatcgcc gccatcgggc
    10441 cataggtttg agtactcagc agacccacac cgaacgcgta cgctgcttta tttaccggac
    10501 cgcccatgtc agtacacatc atgccaccga ggatcgcccc cagcagaacc gcattcgcag
    10561 tccccatggt ctgcagccag tgagtcagcc cttcgagaat gccagcaact ggtttaccga
    10621 tcaggtagat catcgccaga ccgaccacca gactggaaat tagcggaatg atcaggattg
    10681 gtttcagcgc ctccatactc tgtggcagtt tcagttgcgt actgattaac ttcgcaatgt
    10741 aaccagccag gaagcccgca ataataccgc caatgaagcc agaaccggtg ctgacagcca
    10801 gcataccgcc aatcagaccc ggcgtgaggc ccggacgatc ggcaatggag aaggcaatat
    10861 aacctgccag taccggcacc atcagcgcaa aggctgaacc accgccaatc tgcatcagcg
    10921 ccgctgccaa cgtacccggc tctttaaacg cttcgatacc aaaagcaaaa gaaagcgcga
    10981 tacacagacc accagcaacg accatcggca gcatgtaaga aacgcccgtc agcaagtgac
    11041 ggtaagcgcc cgcactctct ttcttacctt cagtggtcgc cgtttgagct ttgcccgccg
    11101 gttcatacgg cgttgcttca gcaaccgctt tatccagttc ctgcgcggtt ttcttcagcg
    11161 ccagaccggt agaggtgcga tacatcggtt taccagcaaa tttcgccaga tccacttcga
    11221 tatctgccgc cacaatcacc agatccgctg cggctacttc ttcgggagtg attgcattac
    11281 ccgcgccaac agaaccacgg gtttcaactt tcacccacca gccacgtttt ttcgcttcgg
    11341 tttcaatggc ttcagcagcc ataaaggtgt gtgctacgcc agtcgggcaa gcagtcaccg
    11401 caactacgcg tttcgggccg ctggcgtcaa ccggtgctgt cgcagcgacc ggcgcagtgt
    11461 aaggtttcgc atggcctttg gcttcactca ggaacagctc agggtgcgca actgcccggg
    11521 aaatatcgcc ctgccagaca tttttaccgt tcagcgcgct gtcgttcggg atggaatcac
    11581 cgagaacaat cgccatttca gcgtcgttcg gattgtcgat gatttccagt tttgcttttc
    11641 gcgccgccgc gcccagcagg gtcttcgcca tataggcgcg tgcctgaccg agattagcgt
    11701 caataatcag cagcgttttc attatgcctc tcctgctgtc agttaaaagg ttgtaagtcg
    11761 acgcgcgcca tcattgcggc caactgcgga cgatcggtaa tacccacatt gctttgactt
    11821 accgccaggg ctgcaacagc agtcgccaga cgcagtgtgt gttcactgga ttcacgcatc
    11881 agcaagccat aaatcaggcc accaaccata gaatcccctg cgccaacggt gcttacgaca
    11941 tcgactgacg gtggtttggc gatccattcg ccggaggcat taacccaaag cgcgccttcg
    12001 gcacccagtg aaataacaac atgcgcaata ccttgttcac gtagcgcatg tgcagcttca
    12061 atcacatctt tcatttcagg cagtttacgg cctgcccaga tttccagctc gcggcggtta
    12121 ggtttcacca gccacggtgc cgctttcaaa cctgctacta acgcttcacg gctactatca
    12181 aagataatgc aaggacactg actacgcagg cgagtcatcc agtcggtgaa cgcttccggg
    12241 ctgacgcctg acggtaagct tccgctgaca cagaccatat cgaactgacc gagccagctc
    12301 agagaatcag tcacaaagcg ttcccagtcg gcgggggtga cttcaaaacc cgagaagttg
    12361 aagtcggtca cttcgccgtc tttttccgtc agcttaacgt taattcgggt gcgcccctgt
    12421 acaacctgga aacggttggc aatgcccagc tcgctgaaca gttgctgaaa accatcctga
    12481 ttgtctttac cgaggaagcc gccaacggtg acatcaattc ccaggtcttt taatactttg
    12541 gccacgttga tgcctttacc cgccgcatgc agaccggtgg ttttcaccag gttcacttcg
    12601 ccgcgttcaa tttccgggca gaaaccaaca aggtcataag ccggattaag ggtgatcgta
    12661 gcaacacgtc tgctcattat gcgccctccc caagaccagc agcgatagcg tcgccaatgg
    12721 ctttcagcgc ctgttcagca tctgcaccct gggcggtaaa gcgtaggcga tgacctttct
    12781 taacgccaag tgccacaact ttcatcagac tacgtccgtt tgccggtttg ccggtgccat
    12841 caaggtttgt cacggtaata tcactgttaa attgtttaat ggtattgacc agcatggtac
    12901 ctggacgagc atgcaggccg tgttcattgc gcaccacaaa ctccgcgctt aacacgtcgt
    12961 cggtcggcgc atcatcgctg gtcagcagcg ccagcaacgt tgccgcatcc gctttcagca
    13021 agcgatcagc tttattgtcg agcaacaaat cagcgagacg cttaagaacc gcgatgggct
    13081 gatcgtcatt catcgccaca ctcaccagca tggctgccgt ttcgccgtcc acatcaaaag
    13141 catttgccgc acggcttacc gcaatcgcgc tacgcagatt gccttcggcg ctatcgctca
    13201 gccagatacc ctgtccgagg ttcagcggtt gttcattgat ggctttggtg acgaaagtgg
    13261 cgtcaactgc ccccgcctct ttcagacgcg cagcgttcag cgcctgaaga gtcagcagat
    13321 cgctggcgac gatatccagc gtcagcattt cgttgtcgag cttcagctgc tcactctgct
    13381 tttcgcccat cagtaatgcg cgaagttctt ctgctgttgt tgctgacttc agttgttcag
    13441 caacggaatc atcgctcagt acgtgggtca gctggcgtag caggcccaga tgttcatccg
    13501 agctggcagc aataccgatt gccacgtacg ctacctgacc gtcaccccag gtgacgcctt
    13561 ccgggaactg aaatacctga acgccggttt tcagcacctg atcgcgggtg tcggtagtgc
    13621 cgtgtggaat agcaatacca ttgccgagga acgttgaggt ttgctgttcg cgcgccagca
    13681 tgccattgac gtagccttct gctacattac cggcctgcac cagcgccgca gcgacctggc
    13741 gaatcgcctc ttctttgtct ccggcctttt cgcccggatg gatgtcctgt acggataact
    13801 ggaacatagt tctcctctct tgctgaattg aaacgattca gcctctatga gaaaaaaagc
    13861 gccaacctgg cttagggtta aagacaagat cgcgctgaaa cgtttcaaga aagcataata
    13921 cttctgtttc agcacgcaag gaaagccgta aagttatgag caaaagtttg atgtgctgca
    13981 caattttttg ccatttttcc taattgctga tgggaaaatc cgttatcagc aatttcattt
    14041 cagcagcgtc agcagccaat cagcaacagt gaaatggcat tttgattatt cgtggcgctt
    14101 ttgatttcgc tgtctgttta ttttctgaca agcagcgtaa actccgcgtc ttcctcttcc
    14161 agtgatcgac cagcatgcat aactcccccg cagtctccag cgcgaaatcg tttgacctga
    14221 cctcgacggc gtttttaatc gttgcctttc tcaccggtat tgcgggcgct ctgcaaaccc
    14281 cgacactcag tatttttctt actgatgaag tacatgctcg tccggcgatg gtgggattct
    14341 tctttaccgg cagcgctgtc attgggattc tggtaagtca gtttctcgcc gggcgctctg
    14401 ataagcgcgg cgatcgcaaa tcgctgattg tcttttgctg cctgttaggc gtgctggcct
    14461 gcaccctttt tgcctggaat cgcaactact ttgttttgct attcgttggc gtctttctta
    14521 gcagctttgg ctcgaccgct aacccgcaaa tgtttgccct tgcccgtgaa catgccgaca
    14581 aaaccggacg tgaggcggtg atgttcagct cttttttacg cgctcaggtt tcactggcat
    14641 gggtcattgg cccaccgctg gcttatgcct tagcgatggg tttcagcttt acggtaatgt
    14701 atctgagcgc agcggtagcg tttattgttt gcggtgtgat ggtgtggctg tttttaccgt
    14761 cgatgcaaaa agagcttccg ctggcgaccg gcacgatcga agcgccgcgc cgtaaccgtc
    14821 gcgatacgct gctgctgttt gtcatttgta cattgatgtg gggctcgaac agcctgtaca
    14881 tcatcaacat gccgctattt attatcaacg aactgcatct tcccgagaaa ctggccggtg
    14941 tgatgatggg gaccgccgcc gggctggaaa tcccgacgat gttgattgcc ggatatttcg
    15001 ccaaacgtct gggtaagcgt ttcttaatgc gcgttgctgc cgtgggtggc gtctgttttt
    15061 acgcaggaat gctgatggcg cattcacctg tcattctgtt gggcttgcag ctgctaaatg
    15121 ctatttttat tggcattctg ggcggcatcg ggatgctcta ttttcaggat ctgatgcccg
    15181 gtcaggcggg ttcagccacc acgctctata ccaacacgtc gcgcgtgggc tggatcatcg
    15241 caggatcagt ggcgggcatc gtcgccgaga tctggaatta tcacgctgtg ttctggtttg
    15301 cgatggtgat gattatcgcc actctgtttt gcttactgcg gattaaagat gtttaaggcg
    15361 cggtcagcat ttccagatcg ataagccagg tcatggcctg ctgacgcgag ttaccgcaca
    15421 tttcagcgct ggcttgtaag cctgcacaga ctttcggtcg cagtggcgag gtgaaaattt
    15481 tacagcgttg ctgttcatca agctgaatgc agggcgtatt ggcgggcttg ccatctggca
    15541 taccgggaat agggctggaa attgaagggg cagtgcaaca cgccccgcaa cccggacggc
    15601 atttcatacg ttgttctctt aaggttaacc accggattga tgagcgcgca cagtaccacc
    15661 ttttttgcac cagcaaaagt gcgaatacca cttgcccgaa aggcccgtcg cgagtacttt
    15721 gtcgcgatat ttttgacatt ttcgactaca ggaatttttc gatgccaaga gcgaacgaaa
    15781 ttaaaaaagg tatggtactg aattacaacg gcaaactgct gctggttaag gatattgata
    15841 ttcagtcgcc cactgcccgc ggtgccgcta cgctgtacaa aatgcgtttt tctgatgtcc
    15901 gtaccgggct gaaagtagaa gagcgtttca aaggtgatga tatcgttgac accgtgacgc
    15961 tgacccgccg ttacgttgac ttctcctatg tcgatggcaa cgaatatgtc tttatggata
    16021 aagaagacta taccccgtat accttcacca aagatcagat tgaagaagag ttgctgttta
    16081 tgccagaggg cggcatgccg gacatgcagg tgctgacctg ggacggtcaa ctgctggcgc
    16141 ttgagctgcc gcagaccgtt gatctggaaa tcgttgaaac cgcaccaggc atcaaagggg
    16201 catctgccag cgcccgtaac aaaccggcga cattgagcac tggtctggtg attcaggtac
    16261 cagaatactt aagcccgggc gaaaaaattc gtatccatat cgaagaacgc cgttatatgg
    16321 ggcgtgctga ctaacttcag ccgcatgcag aaaagggata gctcaggctg tccctttttt
    16381 aatttattat accaatcttc tattttgcgc ttcctgaaca ccttacgccc tcaattttca
    16441 ctcgttgatt gatcgccctc acacttcatc gcattaacaa tccagaccaa tttcaattgc
    16501 tgtcatataa ctttacactg tcattgttaa tttatcgtta ctaagacgtg actcctatga
    16561 agacaattgc ctccgttacg ctcccgcatc atgtacacgc tccacgctac gatcgccagc
    16621 agttgcaatc acgtatcgtt cattttggct ttggcgcctt tcaccgcgct catcaggcgt
    16681 tactgaccga tcgtgtgctg aatgcccagg gcggcgactg ggggatctgt gaaatcagct
    16741 tgttcagcgg tgatcaactg atgagccagc tccgcgcaca gaaccattta tataccgtgc
    16801 tggaaaaagg tgcggacggc aatcaggtga taattgtcgg tgccgttcac gaatgcctta
    16861 atgcaaaact ggattcctta gcggcaatta ttgagaaatt ttgcgagcca caggtggcaa
    16921 ttgtttccct gacgattacc gaaaaaggct attgtattga cccggccacc ggtgcactcg
    16981 acaccagtaa tccgcggatt attcacgatc tacaaacccc tgaagaacct cactccgcac
    17041 cgggtattct cgtcgaagca ctgaaacgcc gccgtgagcg cggccttaca ccgtttaccg
    17101 tgctctcctg cgacaatatt cccgacaatg gccatgtggt gaaaaacgcg gtgctgggaa
    17161 tggcagaaaa acgttcgcca gaactcgccg ggtggataaa agaacacgtc agttttccgg
    17221 gaaccatggt cgaccgcatt gttccggctg caaccgacga atcactggca gaaatcagcc
    17281 agcatctggg ggtaaatgat ccctgcgcga ttagctgcga accgtttatc cagtgggtgg
    17341 tggaagataa cttcgtcgct gggcgtcctg cctgggaagt cgcaggtgta caaatggtga
    17401 atgatgtcct gccatgggaa gagatgaaac tgcgtatgct taatggcagc cactcttttc
    17461 tcgcttatct gggttacctt tcaggattcg cccatatcag tgattgcatg caggatcgcg
    17521 catttcgcca tgccgccaga acattaatgc tgaatgagca agcgccgaca ctacaaatta
    17581 aagatgtcga tttaacacaa tatgcggaca agttaattgc acgttttgct aatccggcgc
    17641 tgaaacataa gacctggcaa atcgcaatgg atggtagcca gaaattaccg caacgtatgc
    17701 tggcaggtat tcgcatacat ctggggtgcg aaacggactg gtcgttgctg gcattaggcg
    17761 ttgctggctg gatgcgttac gtcagcggcg ttgatgatgc cggaaatgcc attgatgttc
    17821 gcgatccgct tagcgataaa attcgcgaac ttgttgcggg cagcagcagt gaacaacgcg
    17881 taaccgccct gctttccctg cgtgaagttt tcggtgatga tctgccagat aacccgcatt
    17941 ttgtgcaggc catcgaacaa gcctggcaac aaatcgtaca attcggcgca catcaggcgc
    18001 tattaaacac cctcaaaatt taacgatttc tgcggttaaa gcggatgaag ctcaccttcg
    18061 tccgctctcc ccttctcttt tctgcctttt ttagccagga ttaacgctca gttaacttac
    18121 cagagtattg cggagccgtt gtgaccagga ccaacctcat caccggtttt ctcggcagcg
    18181 ggaaaaccac gtcgattctt catctgttag cccataaaga tcccaacgaa aaatgggcgg
    18241 tactggttaa tgaatttggg gaagtcggaa ttgatggtgc tttgctcgcc gatagcggcg
    18301 cattgctgaa agagatcccc ggcggctgca tgtgctgcgt taatggttta cccatgcagg
    18361 tagggttgaa taccttactg cgtcagggaa aaccagaccg cttgttgata gagccgaccg
    18421 ggctgggcca tccgaaacag atcctcgatc tgttaaccgc accagtctat gaaccgtgga
    18481 tagatctgcg cgccaccttg tgcattctcg atccacgcct gctgctggac gaaaaaagcg
    18541 ccagcaatga aaacttccgt gaccagctgg ctgccgcaga catcattgtc gccaataaat
    18601 ccgaccgtgc gacgcccgaa agtgagcaag cgctacagcg ttggtggcag caaaatggtg
    18661 gcgatcgaca attaattcac agtgagcatg ggaaagttga cggtcatctt ctggatttgc
    18721 cgcgtcgcaa tttagccgag ttgcccgcca gcgccgcgca ttctcatcag catgtcgtga
    18781 aaaaagggtt agcagcgtta agcctgccag agcatcaacg ctggcgtcgc agtctgaaca
    18841 gcgggcaagg atatcaggcc tgcggctgga tattcgacgc tgatacggta ttcgacacca
    18901 ttggcattct ggaatgggcg cgacttgcac cggtggaacg cgtcaaaggc gtgctgcgta
    18961 ttcccgaagg gctggtgcga atcaaccgtc agggcgatga cctgcacatt gaaacgcaaa
    19021 acgttgcgcc accggacagt cgtattgagc tgatttccag cagcgaagct gactggaatg
    19081 ctttgcagag cacgctgttg aagcttcgtt tagcgactac cgcgtaaggt tgcctgcgtt
    19141 tttcagtaag ataattagag aaaatatgat taaaaatttg ccgcaaatag tgttgttgaa
    19201 tattgtcggc ctcgcgctgt ttctttcctg gtatattccc gttaatcatg gattctggtt
    19261 gccgattgat gcggatattt tttatttctt taatcagaaa ctggtcgaaa gtaaggcctt
    19321 tttgtggctg gttgcattga ccaacaatcg cgccttcgac ggttgttcac tgctggcgat
    19381 gggtatgttg atgctgagtt tctggctgaa agaaaacgcc ccaggcagac gacgtatcgt
    19441 gattatgggt ctggtcatgc tattaactgc agtggtatta aaccagctgg gtcaggcatt
    19501 aattcctgta aaacgggcca gcccaacatt gacttttacc gacattaacc gcgtcagcga
    19561 actgctctct gttcccacga aagatgcctc acgagatagc tttcccggcg atcacggcat
    19621 gatgctgctt attttttcgg cattcatgtg gcgttatttc ggcaaagtta caggtcttat
    19681 cgcccttatt atttttgtgg tttttgcgtt tcccagagta atgattggcg cacactggtt
    19741 tactgacatc attgtcggtt cgatgaccgt gatattgatc ggtttgccct gggtgttgct
    19801 gacgccatta agtgatcgat taatcacctt ttttgacaaa tcactaccgg gaaaaaacaa
    19861 acatttccaa aacaaataac tcacagtaat taacatcatc aggtttattt ttatagtgag
    19921 gataatcctg atgatgcgca ccgtgctttc atctatcgaa cgcaaaaatc attctctaag
    19981 taaatgaatg gattgcatgc gtttcactca atcgtacttt aattgaccaa tcccgcttat
    20041 taactttctg tatcactttt tcttataaaa aatcatgtaa aaccgctcgc caagaccgca
    20101 ccaatcgggt aatctcgaac tcgttttgcc tcggcggtag attatcctca cagcatataa
    20161 ttttgtgcgt tagtccacag atttggcctt aaggaattgt ttcaacatgc ccaggtaatt
    20221 agtctcgtgt cgcttggcat ttttttataa cgatatttgt cgttaaggac ttcaagggaa
    20281 aacaaacaac atggtcaaat ctcaaccgat tttgagatat atcttgcgcg ggattcccgc
    20341 gattgcagta gcggttctgc tttctgcatg tagtgcaaat aacaccgcaa agaatatgca
    20401 tcctgagaca cgtgcagtgg gtagtgaaac atcatcactg caagcttctc aggatgaatt
    20461 tgaaaacctg gttcgtaatg tcgacgtaaa atcgcgaatt atggatcagt atgctgactg
    20521 gaaaggcgta cgttatcgtc tgggcggcag cactaaaaaa ggtatcgatt gttctggttt
    20581 cgtacagcgt acattccgtg agcaatttgg cttagaactt ccacgttcga cttacgaaca
    20641 gcaggaaatg ggtaaatctg tttcccgcag taatttgcgt acgggtgatt tagttctgtt
    20701 ccgtgccggt tcaacgggac gccatgtcgg tatttatatc ggtaacaatc agtttgtcca
    20761 tgcttccacc agcagtggtg ttattatttc cagcatgaat gaaccgtact ggaagaagcg
    20821 ttacaacgaa gcacgccggg ttctcagccg cagctaataa accgtttgga tgcaatccct
    20881 tggctatcct gacgagttaa ctgaaagcac tgcttaggca gtgctttttt gttttcattc
    20941 atcagagaaa atgatgtttc cgcgtcttga tccaggctat agtccggtca ttgttatctt
    21001 ttaaatgttg tcgtaatttc aggaaattaa cggaatcatg ttcatacgcg ctcccaattc
    21061 tggacgtaag ctcctgctta cctgcattgt agcaggcgtg atgattgcga tactggttag
    21121 ctgccttcag tttttagtgg cctggcataa gcacgaagtc aaatacgaca cactgattat
    21181 cgacgtacaa aagtatctcg atacctattt tgccgacctg aaatccacta ctgaccggct
    21241 tcagccgctg accttagata cctgccagca ggctaacccc gaactgaccg ctcgcgcggc
    21301 gtttagcatg aatgtccgta cgtttgtgct ggtgaaagat aaaaaaacat tctgttcatc
    21361 tgcgactggt gagatggaca ttccactcaa tgaattgatt ccggcgctcg acattaataa
    21421 aaacgtcgat atggcgatct tacccggcac gccgatggtg ccgaacaaac ccgcaatcgt
    21481 catctggtat cgcaaccctt tgctgaaaaa tagcggcgtc tttgccgctc tgaatctcaa
    21541 cctgacgcct tcactctttt atagttcacg gcaggaagat tacgatggcg tcgccctcat
    21601 tattggcaat actgcgctat ctaccttttc ttcacgtttg atgaacgtta atgaattaac
    21661 cgacatgccg gtccgtgaaa ctaaaattgc gggcattcct ctgaccgttc ggctttatgc
    21721 agatgactgg acatggaacg atgtgtggta cgcattttta ctgggcggca tgagtggaac
    21781 tgtcgttggc ctgctctgct attacctgat gagcgtacgt atgcgccccg gcagagaaat
    21841 catgaccacc atcaagcgcg aacaatttta cgtggcgtat caaccggtgg tggatacaca
    21901 agctttacga gtcacgggcc tggaagtact gctacgctgg cggcatcctg tcgcgggaga
    21961 aattcccccg gatgccttca ttaactttgc cgaatcgcaa aagatgattg tgccgctgac
    22021 tcagcacctg tttgagttaa ttgcccgcga tgccgcagaa ttagaaaaag tactgccggt
    22081 aggcgtcaaa tttggcatta acattgcgcc ggaccatctg cacagcgaaa gctttaaagc
    22141 agatatccag aaactgctca cttccctgcc cgcacaccat ttccagattg tgctggaaat
    22201 taccgagcgc gatatgctga aagagcaaga agccacacaa ctcttcgcct ggctgcactc
    22261 ggtcggcgta gaaattgcta ttgatgactt cggcaccggg cacagcgcgc ttatctatct
    22321 tgagcgtttt acgctcgatt atctgaaaat tgaccgtgga tttatcaacg ccatcggtac
    22381 ggaaacgatc acttcacccg tacttgacgc ggtgctgacg ctggcgaaac gtctcaatat
    22441 gctgacagtt gctgaagggg tcgaaacgcc ggaacaagcg cgatggctaa gcgaacgcgg
    22501 cgttaatttc atgcaaggct actggattag tcgcccgtta ccgctggacg attttgtccg
    22561 ctggctgaag aaaccgtata cgccgcagtg gtaaggtgtg cttacgtccc ttattattca
    22621 tagtgaaagc atgccggatt gcggctaatg atgagtaaaa ggaaatccgt tgcagatgat
    22681 tgtgcgcata ctgctgctgt ttatcgctct gttcaccttt ggtgtgcagg cgcaggctat
    22741 caaggaaagc tatgcctttg ccgtgctggg cgaaccccgg tacgcgttta atttcaacca
    22801 ttttgattat gtgaaccccg ccgcgccaaa aggtgggcag ataacgttgt cagccctcgg
    22861 caccttcgat aatttcaacc gctatgcact gcgcggcaac ccgggcgcac gcaccgagca
    22921 gctgtacgac acgctattta cgacttccga tgacgaacca ggcagttatt acccgctgat
    22981 tgctgaaagc gcacgctatg ctgacgatta ttcctgggtg gaggtcgcta ttaatccgcg
    23041 cgcccgtttt catgatggtt cgcccattac tgcccgcgat gtagagttta cttttcaaaa
    23101 atttatgacc gaaggcgtgc cgcaatttcg tctggtctac aaaggcacca ccgtcaaagc
    23161 cattgcgccg ttaaccgtgc gcattgagtt agctaaaccc ggcaaagaag atatgttgag
    23221 tctgttttcg ctgccggtat ttccagaaaa gtactggaag gatcacaaac ttagcgaccc
    23281 gctcgccacg cctccgcttg ccagtggtcc gtaccgcatt acgtcctgga aaatggggca
    23341 aaatattgtc tattcccgcg tgaaagatta ctgggcagca aacttaccgg taaaccgtgg
    23401 acgctggaat ttcgacacca ttcgctacga ttattacctc gatgata
//
LOCUS       DAZGAN020000070        22992 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGAN020000070 DAZGAN020000000
VERSION     DAZGAN020000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.070"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(31..1680)
                     /gene="actP"
                     /locus_tag="ACHKYO_RS15300"
                     /old_locus_tag="pgaptmp_003060"
     CDS             complement(31..1680)
                     /gene="actP"
                     /locus_tag="ACHKYO_RS15300"
                     /old_locus_tag="pgaptmp_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HGU4429873.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1677..1991)
                     /gene="yjcH"
                     /locus_tag="ACHKYO_RS15305"
                     /old_locus_tag="pgaptmp_003061"
     CDS             complement(1677..1991)
                     /gene="yjcH"
                     /locus_tag="ACHKYO_RS15305"
                     /old_locus_tag="pgaptmp_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HGU4429874.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2191..4149)
                     /gene="acs"
                     /locus_tag="ACHKYO_RS15310"
                     /old_locus_tag="pgaptmp_003062"
     CDS             complement(2191..4149)
                     /gene="acs"
                     /locus_tag="ACHKYO_RS15310"
                     /old_locus_tag="pgaptmp_003062"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HGU4429875.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4542..5978
                     /gene="nrfA"
                     /locus_tag="ACHKYO_RS15315"
                     /old_locus_tag="pgaptmp_003063"
     CDS             4542..5978
                     /gene="nrfA"
                     /locus_tag="ACHKYO_RS15315"
                     /old_locus_tag="pgaptmp_003063"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HGU4429876.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6023..6589
                     /gene="nrfB"
                     /locus_tag="ACHKYO_RS15320"
                     /old_locus_tag="pgaptmp_003064"
     CDS             6023..6589
                     /gene="nrfB"
                     /locus_tag="ACHKYO_RS15320"
                     /old_locus_tag="pgaptmp_003064"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HGU4429877.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6586..7257
                     /gene="nrfC"
                     /locus_tag="ACHKYO_RS15325"
                     /old_locus_tag="pgaptmp_003065"
     CDS             6586..7257
                     /gene="nrfC"
                     /locus_tag="ACHKYO_RS15325"
                     /old_locus_tag="pgaptmp_003065"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HGU4429878.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7254..8210
                     /gene="nrfD"
                     /locus_tag="ACHKYO_RS15330"
                     /old_locus_tag="pgaptmp_003066"
     CDS             7254..8210
                     /gene="nrfD"
                     /locus_tag="ACHKYO_RS15330"
                     /old_locus_tag="pgaptmp_003066"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HGU4429879.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8230..9948
                     /gene="nrfE"
                     /locus_tag="ACHKYO_RS15335"
                     /old_locus_tag="pgaptmp_003067"
     CDS             8230..9948
                     /gene="nrfE"
                     /locus_tag="ACHKYO_RS15335"
                     /old_locus_tag="pgaptmp_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HGU4429880.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFHFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9941..10324
                     /gene="nrfF"
                     /locus_tag="ACHKYO_RS15340"
                     /old_locus_tag="pgaptmp_003068"
     CDS             9941..10324
                     /gene="nrfF"
                     /locus_tag="ACHKYO_RS15340"
                     /old_locus_tag="pgaptmp_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HGU4429881.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10321..10917
                     /gene="nrfG"
                     /locus_tag="ACHKYO_RS15345"
                     /old_locus_tag="pgaptmp_003069"
     CDS             10321..10917
                     /gene="nrfG"
                     /locus_tag="ACHKYO_RS15345"
                     /old_locus_tag="pgaptmp_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HGU4429882.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASKQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11259..12572
                     /gene="gltP"
                     /locus_tag="ACHKYO_RS15350"
                     /old_locus_tag="pgaptmp_003070"
     CDS             11259..12572
                     /gene="gltP"
                     /locus_tag="ACHKYO_RS15350"
                     /old_locus_tag="pgaptmp_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HGU4429883.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12650..13339)
                     /gene="yjcO"
                     /locus_tag="ACHKYO_RS15355"
                     /old_locus_tag="pgaptmp_003071"
     CDS             complement(12650..13339)
                     /gene="yjcO"
                     /locus_tag="ACHKYO_RS15355"
                     /old_locus_tag="pgaptmp_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HGU4429884.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13433..15580)
                     /gene="fdhF"
                     /locus_tag="ACHKYO_RS15360"
                     /old_locus_tag="pgaptmp_003072"
     CDS             complement(13433..15580)
                     /gene="fdhF"
                     /locus_tag="ACHKYO_RS15360"
                     /old_locus_tag="pgaptmp_003072"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15161..15163),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HGU4429885.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15778..17244)
                     /gene="mdtP"
                     /locus_tag="ACHKYO_RS15365"
                     /old_locus_tag="pgaptmp_003073"
     CDS             complement(15778..17244)
                     /gene="mdtP"
                     /locus_tag="ACHKYO_RS15365"
                     /old_locus_tag="pgaptmp_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HGU4429886.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17241..19292)
                     /gene="mdtO"
                     /locus_tag="ACHKYO_RS15370"
                     /old_locus_tag="pgaptmp_003074"
     CDS             complement(17241..19292)
                     /gene="mdtO"
                     /locus_tag="ACHKYO_RS15370"
                     /old_locus_tag="pgaptmp_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HGU4429887.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAKGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19292..20323)
                     /gene="mdtN"
                     /locus_tag="ACHKYO_RS15375"
                     /old_locus_tag="pgaptmp_003075"
     CDS             complement(19292..20323)
                     /gene="mdtN"
                     /locus_tag="ACHKYO_RS15375"
                     /old_locus_tag="pgaptmp_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HGU4429888.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPFEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20342..20617)
                     /gene="ytcA"
                     /locus_tag="ACHKYO_RS15380"
                     /old_locus_tag="pgaptmp_003076"
     CDS             complement(20342..20617)
                     /gene="ytcA"
                     /locus_tag="ACHKYO_RS15380"
                     /old_locus_tag="pgaptmp_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HGU4429889.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20826..22811)
                     /gene="yjcS"
                     /locus_tag="ACHKYO_RS15385"
                     /old_locus_tag="pgaptmp_003077"
     CDS             complement(20826..22811)
                     /gene="yjcS"
                     /locus_tag="ACHKYO_RS15385"
                     /old_locus_tag="pgaptmp_003077"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HGU4429890.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5191 a         6046 c         6266 g         5489 t
ORIGIN      
        1 cccggtcagg cgaccgggga aggggagaga ttaatgtgcg cggccttgct caacgccaaa
       61 gccggtctgg gagcggataa actgtgcacg gaacagttca cgctcgcgcg caccttccgc
      121 tgagttatcg gttgccgaga agaaccagat gccgaggaat gccacgctga tagagaacag
      181 cgccgggtat tcatacggga agatggcttt ttcgtgacca aggatctgta cccaaatcgt
      241 cgggccgagg atcatcagta ccactgcggt aatcagcccc agccagccac ccatcatcgc
      301 gccacgagtg gtcagtttcg accagtacat agaaagcaga atgatcggga agttacagct
      361 cgctgcgatg gcaaacgcca ggcctaccat gaaggcgatg ttctgattct caaacagcac
      421 gccgaggata atggcaatca cacctaaaat cagcacggtg attttcgata cccgcagttc
      481 ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac aggtcatgcg aaaccgctga
      541 tgcgcccgcc agcgtcagac cagcaaccac cgcaagaatg gtggcaaaag caaccgctga
      601 aataaaaccg aggaacaggt tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt
      661 gttaccacca atcagatggc ccgccgcgtc tttatattcc ggattcgcac caaccagcat
      721 gatcgcgccg aagccgataa taaaggtcag aatatagaag tagcccataa acccggtggc
      781 gtagaacacg ctcttacgtg cttcgcgggc atcgctgact gtaaagaagc gcatcagaat
      841 gtgcggcaag cccgccgtac caaacatcag tcccagaccc agagagagcg cggagatcgg
      901 atctttcacc agcccgcccg gcttcatgat gtcgacacct ttcgggtgta ccgccatcgc
      961 ttcactgaac agattgttga agctaaagcc gacgtgtttc atcaccataa aggccataaa
     1021 gctggcaccg aacagcaaca gcacggcttt gataatctgt acccaagtgg tcgccagcat
     1081 gccgccgaac aggacgtaca tcatcatcag cacgccgacc agcaccaccg caatgtgata
     1141 gttaaggcca aacagcagct cgatcagttt acctgcgccc accatctggg cgataaggta
     1201 aagcgccacc accaccagag aaccacaggc cgaaagaata cgaatcggcc cttgtttcag
     1261 acggtaagag gccacatcgg caaaggtgta gcgccccagg ttacgcagac gttcggcaat
     1321 caggaacaaa atgatcggcc agcccaccag gaagcccagc gagtaaatta agccgtcata
     1381 gccggaggta aacaccagcg cggagatccc caagaatgag gcggcggaca tatagtcccc
     1441 ggcaatcgcc agcccgttct ggaagccagt gatattgccg cctgcggtgt agtagtcgct
     1501 acgagaacgt acgcgttttg atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat
     1561 caggaacata ataatcgcct gccagttcgt tggctggcgc tctacggccc cgctaatagc
     1621 atccgcggcg ttagctgcga aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat
     1681 gatgcttgta cctcatgcag gacttcatta ttaagacggt cgaattcgcc gttcgcccgc
     1741 cagatgtaga taccggtgag aacaaaggag atcacaatca ctccaacacc aattggaata
     1801 ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca gccagccggg cgcgaaggcg
     1861 atcagtaaaa taaagccgat ataaactgcc agcataataa tcgacaggat ggtggcaaac
     1921 cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt cttctatccg ctgataaata
     1981 gtgccattca tcacagattc tccagaggta atgtagggat tgttttaatt cccgctccct
     2041 tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg taggccggat aaggcgttta
     2101 cgccgcatcc ggcaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacaaac
     2161 cgttaccgac tcgcatccgg caattgtggg ttacgatggc atcgcgatag cctgcttctc
     2221 ttcaagcagc ttctcgacta cgccaggatc ggcaagcgtc gaggtatcgc ccaggttgct
     2281 ggtatcgccc gccgcaattt tgcgcagaat acggcgcata attttgccgg agcgggtttt
     2341 aggcagggag tcggtccagt gcagcacgtc tggcgtcgcc agcgggccaa tctctttacg
     2401 cacccagttg cggacttctg cgtacagttc tggtgacggt tcctccccgt gattaagcgt
     2461 gacgtaggcg tagatcgcct gacctttaat attgtgcgga atacctacta cggcggcttc
     2521 ggcaatcttc ggatgcgcca ccagcgccga ctcaatctct gccgtcccca gacggtgacc
     2581 ggagacgttc agcacgtcgt ccacacgccc ggtgatccag taatagccat cttcatcgcg
     2641 acgcgcgccg tcgccgctga aatacatatt tttgaaggtg gagaagtagg tctgttcaaa
     2701 acgttcgtga tcgccaaaca gcgtacgcgc ctgacccggc caggagtcgg tgatcaccag
     2761 gctgccttcg gtagcgcctt ccagcgggtt accttcgtta tcgaccagcg ccggttgcac
     2821 gccgaagaac ggacgtgttg ccgaaccggc tttcagctcg gtagcgccag gcagcggcgt
     2881 gatcatgaaa ccgccagttt cggtctgcca ccaggtatcg accaccggac atttctcgtt
     2941 gccgattttt ttccagtacc actcccacgc ttccgggtta attggctcgc ccacggaacc
     3001 gagaatgcgc agcgacgaac ggtcggtacc ctcgatcgct ttatcgcctt ccgccatcag
     3061 cgcgcggatc gccgtgggtg cggtatagag aatattgacc tgatgcttgt ccaccacctg
     3121 cgccatacgg gcaggcgtcg gccagttggg tacgccttca aacatcagcg tggtcgcacc
     3181 gcaggccagc gggccgtaca gcaagtaact gtgtccggtc acccagccca catcggcggt
     3241 gcaccagtag atatcacccg gatgataatc aaagacatat ttaaaggtca gcgccgcgta
     3301 caccagataa ccgccggtag tatgcagcac accttttggc ttaccggtag aaccggaggt
     3361 gtagagaata aacagcggat cttcggcgtt catctcttcc gcctggtgct gatcgctcgc
     3421 ttgctcaacc aggtcgtgcc accacaggtc gcgcccttcc tgccagtcaa ttttcccgcc
     3481 agtacgcttc agtaccacca catgctctac gctggtgacg ttcgggtttt tcagcgcgtc
     3541 atcaacgttt ttcttcagcg gaatactgcg cccggcacgc acaccttcgt cggaagtgat
     3601 caccagtcgt gagttggaat caataatgcg cccggcaacg gcttccggcg agaagccgcc
     3661 gaaaatcacc gaatgcaccg cgccaatgcg ggcgcaggcc agcatcgcaa ccgcggcttc
     3721 cggcaccatc ggcatataaa tcgccaccac atcacctttt ttaatgccca gctcgagcag
     3781 ggtattggcg aagcggcaga cgtcgcggtg cagctcttta tagctgatat gtttgctctg
     3841 gctggcgtcg tcgccttccc agatgatggc ggtacgatcg ccgttttctt gcagatggcg
     3901 gtcaaggcag tttgccgcca gattcagcgt gccgtcctcg taccatttaa tggacacatt
     3961 accgggggca aaggaggtgt ttttcacctt ctggtaaggt ttgatccagt caagaatttt
     4021 tccctgttcg ccccagaagg tatcaggtac gttaatagat tgttgataca tcgcctcgta
     4081 ctgctgaggg tttatcaggc aacggtctgc gatgttggca ggaatggtgt gtttgtgaat
     4141 ttggctcatg cttttgttct ccttgtagga tgttaataat atgtggcata agcgttaaat
     4201 gtaggggtat tggcagtttt gtttagtatt tgggcgacag atcacgcaaa agtagaattg
     4261 tgcaaataaa cggcagggta atttttgaag gtcagcaaca aaagttgatt aattctttga
     4321 ggaacatgca gttatgcatg ctgttgaaaa agaggaagat actgactaac tctaaagtgg
     4381 tattttacat gcacttacaa ttgattaaag acaacattca cagtgtggtt atttgttaca
     4441 cataggggcg agcaatgtca tgacagtgta ggtgcggtta ctgtcgtgaa aaacaataaa
     4501 aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc tatgacaagg ataaaaataa
     4561 acgcacgccg tatcttcagc ttattgattc cttttttctt tttcacttct gttcacgctg
     4621 aacaaacggc tgctcccgca aaacctgtaa ctgtggaagc gaagaatgaa acctttgccc
     4681 cgcagcatcc cgatcaatat ctctcctgga aagccacctc ggaacagtca gagcgtgttg
     4741 acgccctggc ggaagatcca cggctggtga tcctgtgggc ggggtatccc ttctcgcgcg
     4801 attacaacaa gccgcgtgga catgcctttg ctgtgaccga tgtgcgtgaa accctgcgta
     4861 ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat ggcatgctgg agttgtaaaa
     4921 gcccggatgt ggcgcgtctg atccagaaag acggcgaaga tggctacttc cacggtaaat
     4981 gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg tgccgactgc cataacaccg
     5041 cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct ttcccgtccg tatgcggctc
     5101 gcgcgatgga agccattggt aaaccttttg agaaagccgg acgtttcgac cagcaatcga
     5161 tggtttgcgg tcagtgccat gtggagtatt acttcgacgg caaaaacaaa gcggttaaat
     5221 tcccgtggga tgacggcatg aaagtcgaaa atatggagca gtattacgac aaaattgcct
     5281 tctctgactg gactaactcc ctgtcgaaaa cgccaatgct gaaagcgcag cacccggaat
     5341 atgaaacctg gacagcgggc attcacggta aaaacaacgt gacctgtatc gactgccata
     5401 tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga ccataaaatt ggtaatccgt
     5461 ttgataactt cgcccagact tgtgcgaact gccataccca ggacaaagct gccttgcaaa
     5521 aagtggtcgc ggaacgtaag cagtcgatta acgacctgaa aatcaaggtt gaagatcaac
     5581 tggttcacgc tcacttcgaa gcgaaagcag cgctggatgc aggcgcgacg gaagccgaaa
     5641 tgaagccaat tcaggacgat atccgtcatg cccagtggcg ctgggatctg gcgatcgctt
     5701 cccacggcat tcatatgcac gcaccggaag aaggtttacg gatgctcggt acggcgatgg
     5761 ataaagcggc ggatgcacgc accaaactgg cgcgcctgct ggcgaccaaa ggcatcaccc
     5821 atgaaatcca gatcccggat atctcaacca aagagaaagc ccagcaggct attggcctga
     5881 acatggaaca aatcaaggcc gagaagcagg acttcatcaa aacggtgatc ccgcagtggg
     5941 aagaacaggc acgtaaaaac ggtctgttaa gccaataacc ccgttccgcc tcgcaagggg
     6001 cggaaaacac aatggagtga atatgagcgt attacgttcg ttattaactg ccggggtgct
     6061 ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct acccctgcgg cgcaggcatc
     6121 tgatgatcgc tacgaagtta cccagcagcg taacccggat gccgcctgtc tggactgtca
     6181 taagccagat accgaaggta tgcatggaaa acatgcttcc gttatcaacc cgaataacaa
     6241 actgccggtc acctgcacca actgtcacgg ccagccatca ccgcaacacc gcgaaggggt
     6301 gaaagatgtg atgcgcttta acgagccgat gtacaaggtt ggagagcaga acagcgtctg
     6361 tatgtcctgt catctgccgg aacagttgca aaaagcgttc tggccgcacg atgtccacgt
     6421 aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca cagcaagata cgatgcagac
     6481 gttaagtgac aaaggacgga ttaagatttg cgtcgattgc cacagcgatc agcgcaccaa
     6541 tccgaacttt aacccggcgt ctgttccgtt gcttaaggag cagccatgac ctggtctcgt
     6601 cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca gcggcaccgc agggcgcgtg
     6661 gtggcgaaaa cgttgaatat caatggggtg cgttacggca tggtgcatga cgagtcgtta
     6721 tgcatcggct gtaccgcctg tatggatgct tgtcgggaag tgaacaaggt gccggaaggc
     6781 gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg aatttcctga tgtgaaatat
     6841 cgcttcttcc gtaagtcttg ccagcactgc gatcatgcgc cgtgcgttga cgtctgcccg
     6901 accggtgcgt cttttcgtga tgccgctagc gggattgtcg atgttaatcc tgatctctgc
     6961 gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg tgcgctttat ccatccggtc
     7021 acgaagacgg cggacaaatg cgatttctgc cgtaaaacca atttgcaggc cggtaagctg
     7081 cccgcgtgcg ttgaggcttg cccgacgaag gcgctgacgt ttggcaatct ggacgatcct
     7141 aacagtgaga tttcgcaact gctgcgccag aagcctactt accgctacaa gctggcgctg
     7201 ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg gggaggtgag ccaatgacgc
     7261 agacttccgc atttcatttt gaatcgctgg tgtgggactg gccgattgcc atctacctgt
     7321 ttttgattgg tatttctgcc ggtctggtga cgctggccgt gctgttacgt cgcttctacc
     7381 cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac gctgattgtc gggccgggcg
     7441 cggtgatcct cggtctgttg atcctcgtct tccacctgac aagaccgtgg accttctgga
     7501 agctgatgtt ccactacagt tttacctcgg tgatgtcgat gggggtgatg ctgtttcagc
     7561 tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt tgaacatgat ttgcttgccc
     7621 tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa ggttctgagc ctgctgacgc
     7681 ccgttcatcg cggactggaa acattgatgc tggtgttggc ggtgttgttg ggggcttata
     7741 ccggctttct gctgtcggcg ctgaaatcgt atccgttcct caataacccg atcctgccgg
     7801 tgctgttcct cttctccggc atctcgtccg gtgcggcggt ggcgctgatc gccatggcga
     7861 tacgccaacg cagtaacccg cattccacgg aagcgcagtt tgtacaccgt atggaaatcc
     7921 ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt tgtcggtctg gcgctgggcg
     7981 atgacggtaa agtgcgtgcg ctggtggcgg cattaggtgg cggtttctgg acgtggtggt
     8041 tctggcttgg tgtcgccggg ctggggctga ttgtgccaat gttgctcaaa ccgtgggtca
     8101 atcgcagttc cggcattcct gccgtgctgg cggcgtgtgg ggccagtctg gtcggcgtgt
     8161 tgatgctgcg ctttttcatt ctctacgccg gacagttgac ggtggcgtaa gccagaaaag
     8221 aggtggtttc tggacgtatt ccttcctgaa gtcggttttc tggcgttgtt gttaagtctc
     8281 ggggtcaacg tgttgacccc gttgacggcc tttgcgggag tgcggttgcg ctggcctgcc
     8341 atgatgcgac tcacttgcat cggcattctg gcgcagttcg cgctcctgct gctcgccttt
     8401 ggcgtactga cgtattgttt tctcatcagc gatttctcgg tcatttatgt cgcgcaacat
     8461 agctatagcc tgctgtcgtg ggaactcaag ctggcagcgg tgtggggcgg ccatgaaggt
     8521 tcgctgctgc tttgggtgct gctgctttcc gcctggagcg cgctgtttgc ctggcattat
     8581 cggcagcaaa ccgatccgct atttccgctg acgttagccg ttttatctct catgctcgcc
     8641 gcactgctac tgtttgtggt gctgtggtcc gatcccttcg tgcggatatt tccaccagca
     8701 atcgaaggcc gcgatctcaa tccgatgctg caacatcccg gtcttatctt tcatccaccg
     8761 ctgctttacc ttggctatgg cggtttgatg gtagcggcga gcgtggcgct ggcgagttta
     8821 ctgcgcggcg agtttgatgg tgcctgcgcc agaatttgtt ggcgctgggc actacctggc
     8881 tggagcgcat taacggcggg gatcatcctc ggttcctggt gggcctattg cgaactgggc
     8941 tggggcggct ggtggttctg ggatccggtg gaaaacgcct ctttattacc ctggctttct
     9001 gccactgcgc tgctgcacag tttgtccctg acacgccagc gggagatttt ccgccactgg
     9061 tcgctgttgc tggcgatagt aactctgatg ctgtcgctac tgggtacctt aattgtccgt
     9121 tctggcattc tggtttcggt tcatgcgttc gcactggata acgtccgcgc cgtgccgttg
     9181 ttcagcctgt ttgcactgat tagccttgcg tctctggctc tgtatggctg gcgagcgcgg
     9241 gacggtggcc cggtggtgcg tttttcgggg ttatcgcggg aaatgttaat cctcgctacg
     9301 ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg gaacgcttta tccgatgatt
     9361 tacggtctgc tgggctgggg acgcctctcc gttggcgcgc cgtattttaa ccgcgcgacg
     9421 ttaccgtttg gcctgttgat gctggtggtg attgtgctgg cgacgtttgt ctctggcaaa
     9481 cgcgtgcagc ttccggcgct ggtagctcat gcaggcgtgc tgttatttgc cgcagggatc
     9541 gtggtttcca gcgtcagccg tcaggagatc agcctgaatt tacagccggg tcagcaggtg
     9601 acgctggcag gatacacctt ccattttgag cgcctcgatc tgcaagccaa aggcaattac
     9661 accagcgaaa aagcgatagt ggcactgttt gaccatcagc aacgcattgg tgaactgatg
     9721 ccggagcggc gtttttacga agcacgtcgt cagcaaatga tggaaccgtc aattcgctgg
     9781 aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa ccggagcgga tcgttacgct
     9841 tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg ggggaggatt gttgatgatt
     9901 gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg atgaataaag ggcttctcac
     9961 gctactgctg ttatttacct gttttgcgcg cgctcaggtc gtagacacct ggcaattcgc
    10021 caatccgcaa caacagcaac aggcgttaaa tattgccagc cagttacgtt gtccgcagtg
    10081 ccagaatcaa aacttactgg aatccaacgc gccggtggca gtcagtatgc gccatcaggt
    10141 ttacagcatg gtggcggagg ggaagaacga agtcgaaatc attggctgga tgaccgaacg
    10201 ctacggagat tttgttcgct ataacccacc gttaacgggt cagacgctgg tgttgtgggc
    10261 gctgccagtg gtgttgttac tgctgatggc actgatcctc tggcgagtga gggcgaagcg
    10321 atgaaacagc ccaaaatacc ggtgaaaatg ctgacaacac tcacgatatt gatggtattt
    10381 ctctgtgtcg gcagttatct gttaagccca aaatggcagg cggtacgtgc ggagtatcag
    10441 cgtcagcgcg atccgctaca tcagtttgcc agcaagcaaa ccccggaagc gcagcttcag
    10501 gcattacagg ataaaatccg cgctaatcca caaaacagcg aacaatgggc gttactgggc
    10561 gagtactatc tgtggcaaaa cgattacagc aattcgctgc tggcgtaccg tcaggcgttg
    10621 caactacgtg gtgagaacgc tgaactgtat gcggcgctgg cgacggtgct ttattaccag
    10681 gccagccagc atatgaccgc ccagactcgc gcaatgatcg acaaagccct cgcgctggac
    10741 tctaacgaaa tcaccgctct gatgctgctg gcttccgatg cgtttatgca ggcgaattac
    10801 gcgcaagcca tcgaactatg gcaaaaagtg atggatctca actcaccccg gatcaaccgg
    10861 acacagctgg tcgagtcgat taatatggcg aaattgttgc agcggagatc agattaatca
    10921 tctggtgatg aagattatgt tattgagttg tataaaaaat aatcgaatga acatatgcca
    10981 aaaataatca ccaatcagta ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa
    11041 tggttattca ttaatcctgc cataaactcg cgttattatg cattaatgca gcgaaaagct
    11101 ctgttgttaa agggttgcgc aacataccgc gcaaatgata ctgatcataa gcgttaaaaa
    11161 aatctacaaa ccaacgcaac acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc
    11221 tgaacgggga acggcgctcc attgaggaag ttattctgat gaaaaatata aaattcagcc
    11281 tggcctggca gattctgttt gctatggtgc tgggcattct cctgggaagc tacctgcact
    11341 accatagcga cagccgcgac tggctggtcg tcaatttgct ctctccggcg ggtgatatct
    11401 tcatccatct gattaaaatg attgttgtgc cgattgtgat ctccacgctg gtggtgggta
    11461 tcgcgggtgt gggtgatgcc aaacagctcg ggcgtattgg cgcgaaaacc attatttact
    11521 tcgaggtgat caccaccgtc gccatcattt tggggatcac tctggcgaac gtcttccagc
    11581 ccggtgccgg ggtcgatatg tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca
    11641 ctacggaagc ggtacaaagc agttcccacg gcattatggg cacgattttg tcgctggtgc
    11701 cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct gccgatcatc ttcttctcgg
    11761 tgctgtttgg tctggggctt tcctccctgc ccgcgacgca tcgtgaaccg ctggtgaccg
    11821 tgttccgctc catctctgaa accatgttta aagtgactca catggtgatg cgttatgcac
    11881 cggtgggtgt gtttgcgctg attgcggtga cggtggctaa ctttggtttc tcttctctgt
    11941 ggccactggc gaaactggtg ctgctggtgc atttcgccat tctgttcttc gcgctggtag
    12001 tgctgggaat tgtggcgcgc ctgtgcgggt taagcgtctg gatcctgatt cgtattctga
    12061 aagatgagct gattctggcg tactccactg ccagctctga aagcgtactg ccgcgaatta
    12121 ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac cagcttcgtg gtgccgaccg
    12181 gttactcttt taaccttgat ggttcgacgc tgtatcaaag tattgccgct attttcatcg
    12241 ctcagctgta tggcattgac ctgtccatct ggcaggaaat cattctggtg ctgacgctga
    12301 tggtgacctc gaaagggatt gctggcgtgc cgggcgtgtc gtttgtggtg ctgctggcaa
    12361 cgctgggtag cgtaggtatt ccgctggaag gcctggcgtt tattgctggt gttgaccgta
    12421 tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga
    12481 ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct ggcttatgag cgtgaagtgc
    12541 tgggcaaatt tgataaaact gcggatcaat aattgaagat tgccggggtt atccacccgg
    12601 caatgtgtgg atgcctgatg cgacgctggt gcgtcttatc aggcctacct taaccgttgg
    12661 ttaatttttc aaactcttca cacccggtat caaacccttc catacagctc aggttcaacc
    12721 agtgcagcgc cttttgtttg ttcttctcga taaaaccttc ctcaccgttt aagaacatca
    12781 ttcccgccca gtactcggaa taaccggttc gggaaattgc agagctgcgt ttgaaatacc
    12841 aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt ggcgtaaatc aaaccaagca
    12901 gcatttgggc atcgacggcg gagtcattct ccagatcttc cgaggcgttt tctaacagcg
    12961 aaatcgcctt tggataatcc ggtttacccg cttgagtatt taccagaata tgcgccagcg
    13021 ttacttcacc ttctttactg cccgcttgcg ccgctttctc tgcaagaact tttgcttgtg
    13081 gatagtccag actgaccgga ttggtgattt tgatctgcgc cagcagcgcg caggcatcag
    13141 catcaccgct gtcggcggct ttctgtgccc aatattcggc tttgctcaaa tcgccggagc
    13201 taaaccagct gtcggcaaga aaatattgtg cgcgtcggtc cccggcctct gctgccttta
    13261 aatactggct gccaggctcg gagtcgttcg cgtgggcaaa gaatgtcaaa aacaacatca
    13321 atgcgataat ttttttcatt ttgataatac gattgggtag ggagtaacca gtataaagag
    13381 ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta gaaaggacta tattacgcca
    13441 gtgccgcttc gcgcaggcga gttttcaact tgttgtactc gtcaatcacg tactgctcgg
    13501 cggcgcgctg atcggcgatc ggctcgacgc gcacggcgca gtatttgtat tccggcgttt
    13561 tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca ggcaccaatc caccactggt
    13621 aggtcatgta aatcgcccct ttgttcggac gatcgctgac ctgcgcacgg gtgataattt
    13681 tgcctttacg cgagtgcacc caaaccaatg cctcatcttc aatacccaga cgtttggcgt
    13741 cttcggtatt gatttgtgcg tagccaggtt catcagccag cgccgccagt gccgcacagt
    13801 taccggtcat cgaacggcaa gagtagtgac caacttcacg caccgttgac agtaccatcg
    13861 ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc gcaggtgaag aactgtgcca
    13921 gaccgttcgg ggtatcaaac ttctctttaa acagataaga agtcccctga tcggcatctg
    13981 aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc cattttctcg taagtcgcac
    14041 cgtagaaatc cgggcacaga tgacgcaact catcccagat ctcctgggtg ttgttgtagt
    14101 gcatcggata acccatacgg gtggcgattt cactgatgat ttgccagtcc gttttcagat
    14161 cccatttcgg ttcaactgct ttgaagaaac gctggaagcc acggtcagcc gcagtaaaca
    14221 cgccttcatg ctcgccccac gacgttgacg gcaaaatgac atccgccgcc gacgcggttt
    14281 tggtcataaa gatgtcctga acgataacca gttccagatc ttcaaaggct ttacgtactg
    14341 ccgacagctc cgcgtcagtt tgcagcggat cttcgcccat aatgtacgcg gcacgcactt
    14401 cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata accggtatga gcaggcaggc
    14461 tttccacgcc ccaggctttg gcgaatttct cgcggttagc cggatctttc acgtactggt
    14521 atcccggata cgtatccggc agcgcgccca tatcgcaggc accctgcacg ttgttctgac
    14581 cacgaaccgg gtttacaccc gcatgcggct taccgaggtt accggtcagc atcgcgaggc
    14641 tggtcagaga acgcacggtt tccacgccct ggtagaactg ggttacaccc atgccccaca
    14701 ggatggcggc gcttttcgcc tgggcataca tccgtgccgc ctgacgaatc tcactggcgc
    14761 tgacgccggt gatatcttca accgactccg gcgtgtagcc ttcaacgatt ttacgatact
    14821 cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc gtacagattt tcttcaataa
    14881 tgacatggcc catcgcattc aacagcgcga tgttcgagcc gtttttcagt gcaatgtgca
    14941 tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca gacgataatt ttcgccccgt
    15001 tacgtttagc gttaattacg tgattcgcca cgattgggtg ggaatccgcc gggttgtacc
    15061 cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat agcattgctc attgcgccat
    15121 taccgaccga ttggtgcaga cctgcaaccg atgggccgtg tcagacacga gcgcagcagt
    15181 caacgttatt ggtaccaata acggcgcgcg caaatttttg cattacatag ttggtttcgt
    15241 tacccgtacc acgcgaggag ccggtcgtct ggatggcatc cggaccgtac ttctctttga
    15301 tggcgctcag gcgctcggca acgtaattca gtgcctcatc ccaggaaaca ggttcgagtt
    15361 tgccgccacg ctggcgacgg atcatggggg ttttcaggcg cggggtcagg atctgggtat
    15421 cgttaatgaa atcccagcca taataaccct tcagacacag ggtaccctgg ttggttttcc
    15481 cctgcgctgc ctccgcccgg acgattttgc cgttatcgac gaccaggttg attttgcaac
    15541 ctgatgcgca ataggggcaa accgtgacga cttttttcat cggtctcgct ccagttaatc
    15601 aaatcacgca tacgcgctct cgactacagt atgcatcttt tatgccacat tttatttggg
    15661 gtcattccct gatattacgg gcactattta ttcaaaactc tgacgaaaaa caggctgtcg
    15721 tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc atcacgcggc agacatttta
    15781 ttttttctcg acgacgggag ccgcctgata cccgccgccc agcgatttca tcaactgaat
    15841 gctttggatc acccggcggc tgtccagcat cagtaatgac atctcttcgg caagtactgg
    15901 caaccgggct tcagtggcct gtaagcggct ggttaagccg cgctgatagg cggcctcggc
    15961 agcgcgctgg gtgaagcgcg ttgcttccac gcgttcagcc tgcatttctc gctcgtcgtt
    16021 gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc accgcgttca gtactgactg
    16081 gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg ccttcgagat tggcattcaa
    16141 ccgtccaccg tcaaacagcg gcaatttcag acccgggatg aagttgaact ggcgactggt
    16201 ttttttgaat aaggtatcca ggtggatggc atcaaggccg aaaaacgctt tgatatcaaa
    16261 gctcgggtag aacagcgccc gcgcggaatc cacctgatct aatgacgcct gaacatacca
    16321 gcgcatggct tgcagatccg ggcgtctggc gagcaactca taagagagtg tcgccggaat
    16381 gccggtctgg actcgtggta atgccaccgg tttgatctcc ggcatatcgc tggcccccgc
    16441 gccaatcaat gcacgcagag attctcgcgt ttcggtgatt tgccctttga cggcagcaat
    16501 ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg aaaggcactt gtgcttccag
    16561 accgtgcgcc actttactct ggtgcgcttt caccgcgtaa tcaatcacat cgcgagtttg
    16621 ttctaacaga tcgagcatct gatagctggc ctgcatactg taataaagct gcgctacgcc
    16681 cgtggtcagc gatagctcta ctgctgcggt ttctgccagc gcggcattat gcgcgccaat
    16741 ggcggcggca accgctgagc gatgcacacc ccacaaatca agatccagtc cggcaaacaa
    16801 acctactgtg gcttccgtat agtacggccc gtccataccc agtgcgggcg catccatcgc
    16861 ataagggctt aaaaagccgt tcgccgagac gcgttggcgg ttgagcatcc ctaacgctgc
    16921 gacctgtaat tgtgaaccgg catctaacaa atcggcctgg gactgcgctt tttcttcccg
    16981 cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc cgttggatca gcgcatccag
    17041 ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc cagccggagc tggcaagatg
    17101 aatatcgtcg gccagtttga tttgttccgg tttgagctgt tgatgaggtg cagaatcctt
    17161 acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg agaatgctgc acaacagcag
    17221 acgtgaaagt tgacgattga tcattgcgtg gctccttgtg cctgttccgt ggcgggcgtt
    17281 aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct ggcagacgtg ttgctcctgt
    17341 tttaataagg tgggcaggat ggcctgcgag gcgggtgtct cttcaagcgt tatttgagga
    17401 gaacggctga gtgcggttgc cagaccggca gcgtatttct ccagggcgtc ggcaaactga
    17461 gctgctctgt ccggctgtag tgcgttatcc agcgcctgcg ccgagttcca ggtggcatcc
    17521 caggcgtgaa gaatatcgcg gccctgacga ataaccgttt gcgaacgttc aatcagtaag
    17581 gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc gttggcacat ctcttcacag
    17641 gcattaaacg ccgcatgcag accgatacga atttgcagat aagtgcgcag agccgtgact
    17701 tcctgctggc gtgggatccg cattacttta cttaacatac ccagcgtgcc agccagtttt
    17761 tgcggcagtg tgcgcgcttc actttcaggc cagacaaagg tgtaaatcac cgcggacacc
    17821 acggtaccaa tgatgatacc catggcgcga tcgcgaattt ccaccaggtc gtacaccggg
    17881 ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca ccatctgtgt gccgatataa
    17941 gaagagcgtt cagagctggt ggcaatccat gcgcccaaca ggaaaatcgg tgccagcaca
    18001 aacagcaatt cgacaatatt gtccagccag ggcatgacca gtagcgtgaa taacagcgcc
    18061 agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca tcttctggta cgacgaaccg
    18121 acatttgggt tagcgacgat cacgcatgtc agcatacagg tgtgaatgcc ttcccagtcc
    18181 acgccgctgt agaaggtgta acagatcaaa caggcgagca gtgtttttac cgcgtagcgc
    18241 atatagtctg gattggtaaa agcatcggcg accattgatg gcggtttggc ggcgggcgtt
    18301 ggcggcgtat tcgggtccat ctggcccagt tgtaacaacg tctggcagat attctccagg
    18361 ttacattccc gtgccgccat cgcttcactt tcagtgatcc gccagtcgct ttgccagcac
    18421 tgacctttag caacggcatg ctgcagctta ttgatttctg aggctaattt ttgccgaaat
    18481 tcaataattg cctgagaatc agcaaaagag gtgggatcgt agcgattcag cgtcgagtaa
    18541 atgtaggtta ccgttgccac gcagctttgc caccatgcgc tttgagttcg ccagttggca
    18601 tcgtccgcga gacaaaagac attcagtttt tgcagcgcca gcgcctctct ttcaatccgc
    18661 gtttcgggta gcggtgcgag gctgtccgtc aggtgactaa tggcatcatc aagccgatca
    18721 ttaagcgctt gatgcatttg cgtaatggca cgactgggaa accacagcac gccgattaac
    18781 gtcatcagca aggttggata gaggccaaca acgatacacc acagcgttaa gcgcacgacc
    18841 acttccggat agtcgagcat ggcggggaag gtttgcccgt aaatggcgac aatggcgaca
    18901 gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa acatacagcc catcaggatc
    18961 ggcccggcga tgatcaatcg gatcaacggt tcgccgtatg accatttgta gatcaaaaac
    19021 aggctgccga tctccagcac cgtggcaacc acaaacaaga tcgcgacaaa tttggtgtaa
    19081 aacgcgttcg actgaatacc gtaaaacagc actgccagcg ataacgccac aaaagggatc
    19141 tcaaaagtca tcgagatcag aatcaccagc aggcagccta cccagagttg cacgatctgc
    19201 ggcacgcggc cgggtcgccg ctcgcttaac tcttcatgaa agaacgccag cagcctgacc
    19261 accggtaatg gcagggagtt gagcgcgctc attattgcgg ctcaagatta gcgactgccg
    19321 aagcgccgat gcggaacatt tccgggtcag gtttatcgac catgattttg accggaaaac
    19381 gctgggcaac gcggacccag ttaatagagc gagaaacttt cggcaggccg cccagcacca
    19441 ggccgccgtc atccggtagc acgccgtagc caatcgaatc cactttaccc tcgaaggttt
    19501 tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc tgagcgaata tttttcagat
    19561 cggtttcgcg gaagttggcg atcacgtacc agtgacgagt gtcgattagg gtaaaaatag
    19621 ggcgcatggc agaagcaaat tgcccgacgg aggttttgag ggaaatgacc cggccatcaa
    19681 acggcgcgcg aacggtcgcc atttccagat gaagtttggt cagggcaata tccgcttcga
    19741 ccgccgcacg ttgcgccacc agcgcatcga cgccgctgac ggcgctggcg gctgactgcg
    19801 cctgtaacaa tacggcatta agatcggctt ctgcggcgcg ctgcgcagtt cttgcacggt
    19861 caacgtcttc cgctgagaca aaaccctctt tcagtaatgg ctcggtgcgg cgtaatgtat
    19921 ctgtggcctg tttcgcggcg gcacgggctt tttctaccgt ggcattaatc gagtcggcac
    19981 caaactgttg cgcatcaacg ctacgctggg tgagcataat ttgcttatcc agcgccgcga
    20041 gggaggcttc agctttcgcc agattggctt cgaacgggcg cgggtcaatg cggaacagca
    20101 aatcgccctg tttgactgcc tggttgtcgg tgaccgccag ttctacaatg cggccgctga
    20161 cttccggcac cacatcaatg gtatctgctg acgcgtaagc gtcattagtt gatggcgcac
    20221 tgtctacgcg ccagataacg aaaacaaggg caaccagcgc caatgcaacc actaacagag
    20281 cagggaattt actgcgagga gcttttttcg gcgtactttc catgagcgcg tctcaaatag
    20341 attagaaaaa tgccagccag aacagcatgg cgtagagagc aaaaagggcg gtataaataa
    20401 ttccgacaaa tgccagattg atatttgccc gctgaataac tcgcctcgtg atgagcatca
    20461 aaataaggct ggcaatggcg cagaaaaacc agctgggata ataagcaccg atcaccggga
    20521 ttgccggaga taatgagcat cccgaaacca tgaagacggg aattatccag gctgtttttt
    20581 tgagttgcat cgccatgcga gagagaactg ttggcatatt aaatacatcc tgtctgctgc
    20641 gaacaatatc tgtagtgaat gtcaatatcc attgaaggcg tcagtatggc tattttctcg
    20701 atgaataaaa atagctttat tcagccaccg cattcataac ttatatattt tatttattaa
    20761 ttggctgtat atatttttaa aataaatgct tttgggggct tcagcacccc caaaagcgag
    20821 tgacattaat ttggggttac gatattcacc cacaaatcga aattatccag acaggcgata
    20881 atttcttcca gtttcgcgcc attgccaata attttggctt tcttcgcttt taccagatcc
    20941 gccattttgg cttgcccggt cagcacggcg tgcagatctt cacggctgat gtagaaagag
    21001 gcgtcagctt gggattgcag tgttttacgg tagttaagca cgctatcgtt cagcgtcagg
    21061 ttgaggttat cgccattgct catattgaag ttcaggctga tatttttacc cgcggctttc
    21121 gcgctatcga ggcgaacgga cataaagtcg aacagcattt cgaccgacat cccgcgaatg
    21181 gtgtccgggg aaccggtggt gccgtggctg aacttatgca ccccttcgcg cagctctttc
    21241 gcgccggtca ggtagaaacc gcgccaggtg gcggactcgg cctgatagcc cagctgttca
    21301 aagttattcg cttgcagatt cttcgcgacc tggtcacccg ggttggcggc aatcacctgt
    21361 ttcagcagtt ctgccgacca gcggtaatca ccttgcttgt tcgcttcttg cgccaggttg
    21421 atgacacggg cagaaccgcc cagcgcctgc acgtaacgtt tacccatctc cacctgacca
    21481 tacggatgca ggttagccgg gttaccgtcg taatagccaa gatagaagtt atacaccgcg
    21541 cgggcgttgt ggctgacaga accgtaatag ccacggctgg cccagttatt ggcaagtgca
    21601 ggcggcagtt taatcatgtc gccgatttca ttcatggtgt agccctggtt cgccaggtgc
    21661 agggtctggt cgtgaatgta cttgatagta tcgcgatatt taccaatata atcattgata
    21721 tgcttattgc cccagaccgg ccaggtgtgc ggcataaaca ggacttccgc gtcgttaccc
    21781 cacatatcca gcgtttcgtt cagatactcg gtccacttgc tggtgtcgcg ggttttcgcg
    21841 ccgcgcagag tgtagaagtt gtgcagggta tgcgtggcgt tctcggcggt acacagggct
    21901 ttcagggccg gaatatagaa gtgcatttcg gctggcgctt cgctacctgg ggtcatcagg
    21961 aagtcaaact ccaggccgtc gataatcatc ttctcaccgg ttttggtgat ggttttgttc
    22021 ggtgcgatga tgctcgggta gcccgatgcc agcgttacgc caagaccatt gccgatgtta
    22081 ccctgagggt tatgcgccag cagcaggccg taggagtaaa gtgcacggcg gctcataata
    22141 ttacccgcca gtacgttttc gctgatggct tcgtccataa agccagcagg ggcgataacc
    22201 tgcactttac cggatttcac atcggcttca gagataatgc ctttcacgcc gccatagtgg
    22261 tcggtatggc tgtgggtata aataaccgca acaatcggtt tttgcgggcg attttggaag
    22321 taaaggtcaa gggcggcttt tgcgctcggt ggcgtcacca gcgggtcgat gacaataatc
    22381 ccggtatcac cttcgataaa ggtgatgttg gagatatcct gaccgcgaac ctggtacatt
    22441 ctgtcggtga ctttaaacag gccggaaata ccgtttaact gcgactgacg ccacaggcta
    22501 gggttaacgg tttcgggcgc gggcgcattg atatcaaatt tataatcttc cccgcgataa
    22561 tatggtttgc cattagcatc gcgcagaata ccttcatcca gcagcggagc gataaatcca
    22621 cgctgagcgt cgtcaaaatc ctgacgatcg ctaaacggta atgatttggc gtaattctga
    22681 ttaacctgtt gggtatattg agtggcggct ttcgcttcat ctgttgcgta agcggtattt
    22741 accatcatgc cgctggcggc agttaacgca ataacaattc tgctcaaacg gaataaccga
    22801 gagttattca tataaatctc catttatcat ttatgtcgta aatatgtaat ccatgcaaaa
    22861 tacgtcattt aaagctgggg ttatcataat tgggaatttg cgactttaat aagtggaagt
    22921 gtgagcggaa cgatccattt tattaggcat ttgtgctgaa gggggaaaat tacgcgaatt
    22981 tattatctct gt
//
LOCUS       DAZGAN020000071        22735 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGAN020000071 DAZGAN020000000
VERSION     DAZGAN020000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.071"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..163)
                     /locus_tag="ACHKYO_RS15390"
                     /old_locus_tag="pgaptmp_003078"
     CDS             complement(<1..163)
                     /locus_tag="ACHKYO_RS15390"
                     /old_locus_tag="pgaptmp_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HGU4429891.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFG"
     gene            complement(237..1181)
                     /gene="yfdV"
                     /locus_tag="ACHKYO_RS15395"
                     /old_locus_tag="pgaptmp_003079"
     CDS             complement(237..1181)
                     /gene="yfdV"
                     /locus_tag="ACHKYO_RS15395"
                     /old_locus_tag="pgaptmp_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HGU4429892.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(1251..2945)
                     /gene="oxc"
                     /locus_tag="ACHKYO_RS15400"
                     /old_locus_tag="pgaptmp_003080"
     CDS             complement(1251..2945)
                     /gene="oxc"
                     /locus_tag="ACHKYO_RS15400"
                     /old_locus_tag="pgaptmp_003080"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HGU4429893.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQILFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYVIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(2999..4249)
                     /gene="frc"
                     /locus_tag="ACHKYO_RS15405"
                     /old_locus_tag="pgaptmp_003081"
     CDS             complement(2999..4249)
                     /gene="frc"
                     /locus_tag="ACHKYO_RS15405"
                     /old_locus_tag="pgaptmp_003081"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HGU4429894.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(4762..5394)
                     /gene="yfdX"
                     /locus_tag="ACHKYO_RS15410"
                     /old_locus_tag="pgaptmp_003082"
     CDS             complement(4762..5394)
                     /gene="yfdX"
                     /locus_tag="ACHKYO_RS15410"
                     /old_locus_tag="pgaptmp_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HGU4429895.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNNDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            5690..5965
                     /gene="ypdI"
                     /locus_tag="ACHKYO_RS15415"
                     /old_locus_tag="pgaptmp_003083"
     CDS             5690..5965
                     /gene="ypdI"
                     /locus_tag="ACHKYO_RS15415"
                     /old_locus_tag="pgaptmp_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HGU4429896.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(6042..6284)
                     /gene="yfdY"
                     /locus_tag="ACHKYO_RS15420"
                     /old_locus_tag="pgaptmp_003084"
     CDS             complement(6042..6284)
                     /gene="yfdY"
                     /locus_tag="ACHKYO_RS15420"
                     /old_locus_tag="pgaptmp_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HGU4429897.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            6637..7557
                     /gene="lpxP"
                     /locus_tag="ACHKYO_RS15425"
                     /old_locus_tag="pgaptmp_003085"
     CDS             6637..7557
                     /gene="lpxP"
                     /locus_tag="ACHKYO_RS15425"
                     /old_locus_tag="pgaptmp_003085"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HGU4429898.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFINPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            7913..7984
                     /gene="ypdK"
                     /locus_tag="ACHKYO_RS15430"
                     /old_locus_tag="pgaptmp_003086"
     CDS             7913..7984
                     /gene="ypdK"
                     /locus_tag="ACHKYO_RS15430"
                     /old_locus_tag="pgaptmp_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HGU4429899.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(8049..9287)
                     /gene="alaC"
                     /locus_tag="ACHKYO_RS15435"
                     /old_locus_tag="pgaptmp_003087"
     CDS             complement(8049..9287)
                     /gene="alaC"
                     /locus_tag="ACHKYO_RS15435"
                     /old_locus_tag="pgaptmp_003087"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HGU4429900.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            9664..11361
                     /gene="ypdA"
                     /locus_tag="ACHKYO_RS15440"
                     /old_locus_tag="pgaptmp_003088"
     CDS             9664..11361
                     /gene="ypdA"
                     /locus_tag="ACHKYO_RS15440"
                     /old_locus_tag="pgaptmp_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HGU4429901.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            11376..12110
                     /gene="ypdB"
                     /locus_tag="ACHKYO_RS15445"
                     /old_locus_tag="pgaptmp_003089"
     CDS             11376..12110
                     /gene="ypdB"
                     /locus_tag="ACHKYO_RS15445"
                     /old_locus_tag="pgaptmp_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HGU4429902.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            12123..12980
                     /gene="ypdC"
                     /locus_tag="ACHKYO_RS15450"
                     /old_locus_tag="pgaptmp_003090"
     CDS             12123..12980
                     /gene="ypdC"
                     /locus_tag="ACHKYO_RS15450"
                     /old_locus_tag="pgaptmp_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4429903.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(12983..15478)
                     /gene="ptsP"
                     /locus_tag="ACHKYO_RS15455"
                     /old_locus_tag="pgaptmp_003091"
     CDS             complement(12983..15478)
                     /gene="ptsP"
                     /locus_tag="ACHKYO_RS15455"
                     /old_locus_tag="pgaptmp_003091"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4429904.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAALFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(15503..16540)
                     /gene="ypdE"
                     /locus_tag="ACHKYO_RS15460"
                     /old_locus_tag="pgaptmp_003092"
                     /pseudo
     CDS             complement(15503..16540)
                     /gene="ypdE"
                     /locus_tag="ACHKYO_RS15460"
                     /old_locus_tag="pgaptmp_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(16540..17625)
                     /gene="ypdF"
                     /locus_tag="ACHKYO_RS15465"
                     /old_locus_tag="pgaptmp_003093"
     CDS             complement(16540..17625)
                     /gene="ypdF"
                     /locus_tag="ACHKYO_RS15465"
                     /old_locus_tag="pgaptmp_003093"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4429905.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(17640..18887)
                     /gene="fryC"
                     /locus_tag="ACHKYO_RS15470"
                     /old_locus_tag="pgaptmp_003094"
     CDS             complement(17640..18887)
                     /gene="fryC"
                     /locus_tag="ACHKYO_RS15470"
                     /old_locus_tag="pgaptmp_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGU4429906.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(18909..19235)
                     /gene="fryB"
                     /locus_tag="ACHKYO_RS15475"
                     /old_locus_tag="pgaptmp_003095"
     CDS             complement(18909..19235)
                     /gene="fryB"
                     /locus_tag="ACHKYO_RS15475"
                     /old_locus_tag="pgaptmp_003095"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGU4429907.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            complement(19454..20419)
                     /gene="glk"
                     /locus_tag="ACHKYO_RS15480"
                     /old_locus_tag="pgaptmp_003096"
     CDS             complement(19454..20419)
                     /gene="glk"
                     /locus_tag="ACHKYO_RS15480"
                     /old_locus_tag="pgaptmp_003096"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HGU4429908.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            20623..21879
                     /gene="yfeO"
                     /locus_tag="ACHKYO_RS15485"
                     /old_locus_tag="pgaptmp_003097"
     CDS             20623..21879
                     /gene="yfeO"
                     /locus_tag="ACHKYO_RS15485"
                     /old_locus_tag="pgaptmp_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HGU4429909.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            21994..22320
                     /gene="ypeC"
                     /locus_tag="ACHKYO_RS15490"
                     /old_locus_tag="pgaptmp_003098"
     CDS             21994..22320
                     /gene="ypeC"
                     /locus_tag="ACHKYO_RS15490"
                     /old_locus_tag="pgaptmp_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HGU4429910.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(22461..>22735)
                     /locus_tag="ACHKYO_RS15495"
                     /old_locus_tag="pgaptmp_003099"
     CDS             complement(22461..>22735)
                     /locus_tag="ACHKYO_RS15495"
                     /old_locus_tag="pgaptmp_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HGU4429911.1"
                     /translation="PLWVRRTVTMLPSFIVILMGLDPTRILVMSQVLLSFGIALALVP
                     LLIFTSDSKLMGDLVNSKRVKQTGWVIVVLVVALNIWLLVGTALGL"
BASE COUNT         5726 a         5879 c         5476 g         5654 t
ORIGIN      
        1 gaccaaatgt gcgggtatca tcaccatgac ccggcggctc aactttaatt acccttgcgc
       61 ccatattaca aagaagttga gttccgaaag gtccattaag gacatgtgtc atatcgataa
      121 ctaataagcc ttcaaacggc cctttgcttt cattatttgt catttgctta tgccttgaaa
      181 aaagccgaag gtgaaaagac acttcggcat ttatgtaaaa tcaataacat gattaattat
      241 gaaaccagtc gactgacata aatccacaag ggagccgtga cgacaaagcc cagtacgctc
      301 acagccaatg acgctgtacc agtacgggtg tagacattaa accggctggc aataatgatt
      361 ccggagaatg ccggcggtaa tgcgcctgcc agtaccatca tctgcagatg ttcgctgttc
      421 aaatgacatg ccataccaac gagaagcagt gccagtggca tcagaatcag cttcaggaag
      481 gtgttataag caatttcggc actgaactcg aatttatgtg cagccagagt caaccccgca
      541 gcgaataccg ctacccctga gttagccttc gcaatcagat taaaggttgg gtcccatgct
      601 gccggaattt ttaccccaac caacaccagg atcgttgcca gaacaggtgc ccatactact
      661 ggctcctttg ccgcagaaat taatgcgctc agattactat tcttcttacc atccgctcct
      721 gaggaaggat tcagcaaata cagaccaata ggaatagtaa ttgcgttaac aataatagaa
      781 ataattgcta ccactaaacc tgttgatacg gaatcaccat aaataggatc gagaactgca
      841 aaccccagga atccaatggt aggtgaaccc gcaattaatg cacatacagc tgcttctgca
      901 tgggtacgtt taaaaaattt gtagcaacca aaccaggaga aaaagaaaca tccgacaata
      961 accacaagtg atacaagggt cagacgagtg tccgcaaaaa tcatttccct gtttgcccgg
     1021 gtaatagaaa caaatagagc cgcaggaaga gcgtagttta ataccagttt attaaatgcg
     1081 cgggcttgat cttctgaaaa agtttctcgt ctgccgctaa aataacccaa cagcatgatt
     1141 acgataatag gcaataaatc gccaataaaa aatgttagca taatagtatt tccgataagg
     1201 gtcccctcac catcgcataa tgagttggca agaggagacg atgtcagtat ttaattacca
     1261 gcgacttgtt ttgggttaag tttggtaata tggccacttt cagttcctgc tgcagggtcg
     1321 atgaccacat taataatggt cggtttgcgc gactgaatac cggtggttaa agcatgacga
     1381 agttcatctg tcgtggtgac gttatagcca acgccacgaa acgcatccat taatttgtca
     1441 taccttgcat ggtgcaacag atccgttggt gatggtgcac cagcgccact gagatcaaca
     1501 ccgtctcctc tgtagatgcc gccattatta aaaataacga tcgtcaccgg caggttatat
     1561 cgacaaatcg tttcaatttc catcccactg aaaccaaaag cactatcacc ttcaatggcg
     1621 acaaccggag aaccagaagt cacgctagca ccgatgacat agcccatacc gatgcccatg
     1681 acaccccagg taccacaatc cagacgacga cgtggtttat acatatcaat aatatttcgt
     1741 gcattatcca gggtatttgc accttcatta actaaataaa tatcctggtt ctcgcgcaat
     1801 acgtcgcgca cagcacttaa tgcattaaag taatttaatg gttgagtatc tgtacttaat
     1861 ttttcatgca ttttttgtgc attttgctgc ttgtggatat ttaaaatatc gcgccatacc
     1921 agtggagtcg taaatgtgtt ttgtttcagt tctgccagca taccttgcat actggatgca
     1981 atatcaccaa cgactggcac agcaatgggg cggttgctgt caatttcctg cggttcaata
     2041 tccagttgaa taaactgtgt atctgccgcc catccttttt taccgtgtgc caataaccaa
     2101 ttcagtcgtg caccaacaag catgacaacg tcagcatttg ccagggcaaa cgaacgcgca
     2161 gctgccgcag aaagtggatg cgtatcttca aggatccctt tcgccataga cattggcagg
     2221 aatagaatct gagtactttc aataaattca cgaagctgtt catcagcttg tgaatacgcc
     2281 gcgcctttgc caaggataat taatggccgt tcagcttttg ctaaaagcga aattgcgcta
     2341 gtgactgact tcgggcatgg caataatgct ggcgatggat tttcaacttt aacaatcgtg
     2401 gttaacgctt cgtctttttc catcgtcgcg gccaggacat ttgctggcaa atcaagataa
     2461 actccgccag ggcgacccga tacagagacc cggatagcgc gtgccaatgc aatgccaaga
     2521 tcctgcggct gattaacgcg aaatgctgct ttggcatacg gttttgccgc attcatttgg
     2581 tccagctctt cataatcacc ttgctgtagg tcgacgatcg cgcggtcgct ggagccgcta
     2641 atcatgatca tcggaaaacc atttaccgtt gcgttggcca atgcggtcaa accattgagg
     2701 aatcctggcg cagaaactgt caggcagatc cccggttttt gggtaagaaa accgcttgcc
     2761 gcagcggcat agcctgccga ctgctcatga cgaaaaccaa tataacgaat gccttccgcc
     2821 tgggcatggc gtgccatatc cgtcacagga atacctacaa caccataaat agtgtcaata
     2881 ttattctgtt ttaatgcttc aacgatgata tgcataccat ctgtcatttg aagttgatct
     2941 gacatgatta ataacctcga aatttttata aattgggagc cagaagcccc cgttaatatc
     3001 agatggcgtg gttttgcttc attgcagcaa tttcatcgtc gctataaccc agctcctgca
     3061 atacagcagc ggtatgttca cctaatagcg gcgcagcttt aatatccggc gtaaaggcag
     3121 agaatttcat tggacaacca acggtcagat attttccacg caacggttgt tccacttcaa
     3181 caacactgcc actttggcgc aaagagggat caagtgaaat ttctttcata cttaaaaccg
     3241 gtgcacaagg aatatcaaac tgagtcaaat aggccaccgc ttcatgttta tcaatagtga
     3301 cagtgtattt ttcgatttca gcaaaaatat cgaaaatatg tggctgtcgg gcatgggctg
     3361 tactgtatgc cggatcggta atccattctg gtttgccgat ggctttacag gtgttttccc
     3421 agttttgctc ctgaatagtg aaataaatat aggcgttagg atcggtttcc cagcctttac
     3481 atttcaggat ccagccaggc tgaccgccac cacctgcatt accaccgcgg ggaactgcat
     3541 caccaaatgt accattcgga tactgcgggt attcttccag ataacccaat ttatcgagac
     3601 gctgctggtc acgtaatttc acgcggcaaa ggttcaatac ggcatcctgc attgacatgg
     3661 tgactcgttg cccacgcccc gttttttcgc gatgcagcaa agcagcaagt aaaccgatca
     3721 gcaaatgcat tccggtgttg ctgtcaccca acgctgcagc gcttaccagc ggcggaccat
     3781 cccaaaaacc cgtagtggat gccgcgccac ccgctgcctg agcaacgttt tcataggctt
     3841 ttacattcac ataaggcgaa cactcatcaa accctttgat cgaaccaaaa atcagacgtg
     3901 gattgatttc ttgaatatgc tcccaggtga agcccatgtg atcaatggcc cctggatgaa
     3961 agttctcgac taagatatca gcttcgcgga tcagcttttc cattacctct ttgccttccg
     4021 ctgttttggt atttaactca atagaacgtt tgttactgtt aagcatggtg aagtaaagcg
     4081 catcgatatc aggaatatct cgcagctggt gacgcgttac gtcaccaacg ccgggacgtt
     4141 caattttaat aacgtcagcg ccaaaccagg ccagcatttg agtacaagat gggccagatt
     4201 gcacaccggt gaaatcgaga actttaattc cttgaagtgg agttgacata gcaataccta
     4261 ttagttaatt aaatatgtgt taatttagcg tttatttaag gcgtaatatc ttctataaat
     4321 aacccgcaaa atatcgtcgt agctgcactc gatattttta aaatcctgta tcaagtgctg
     4381 aaaatcattt catcgccact tccaattcct ttctgcatgg taagtagtga ggacgaaaag
     4441 aagaatatga taaatattga ttcgtggtaa tcagagagag tcgcgacatt tgtgatgtta
     4501 ttcttacaac tgtaagaagc gccggtgatt tacaagtaag ggtatgatta cgctttttaa
     4561 agcgtaccat aaaacataac ataataaatg atttgtgatg acacgggatt attgggattt
     4621 actttgagga agataaaatg cgtgtttata gaatcgcgag cgccaaataa ttttatgacc
     4681 acaacctgtc gtattgtggt cataaaaaat tgtcggctgg agggagttaa atcctttacc
     4741 tcaatatact gcctgtcagc attagttcac aaacagtgct tcgctgtcga cgatgattcc
     4801 atcttccgca cctttaagcg ccaggtttgc ctcgtagtat tgctttttat caagcagttt
     4861 ctgcgcatca gcaagtgcat tacgagtctg tttaagcggc atcagatatt ggttttccat
     4921 tacgccaaca ccggccaggc gaagttcttc cattgcgccc tttttatccc ctttcgccat
     4981 tttttcattg gcaattttta tcgcggcttc tttttcggga gttgctacat aactctcaga
     5041 aattccgaca gatgcattaa tgacaatata ttggtcatta ttgagattgg tctttttacc
     5101 tggcttagcg aactttgccc attcggtact gtcatctgac agcaaagcgg aagcttcatt
     5161 ggtcagttct tttgcctttt ctggatcgcc atgaaacaga gccaggcgcg ctacctgcac
     5221 gtcacgcatc gcatataaac cttgttcaga gatacgctct gctgctgccg ttttctgggt
     5281 ttgctgaact tgctgagttt gctgttgtgc cgctaccggt gcattatcag cagcccatac
     5341 agttgaagat gccagaattg ctgtgaccat cgtagccata attaaacgtt tcatacttac
     5401 ctcattcttt aacagtttta gaaaacgccg ccatcatatt gggtgagcat gaaatgagaa
     5461 atcagagagc aacggtcatt ttgaaagggt tatcttacag ttgtagttat tatcgcaaga
     5521 agtattttag tcttacaatt gtaggaatct gcttcctgtc gcagagaact attcttatgt
     5581 accacaaaga gatgtcattt attatatcga tgtaaatatt gattaattat atgtaaatac
     5641 agccataaca tcgtaggttc tcagtgaacc attttcagga taactttcta tgaaagtaaa
     5701 cttaatactt ttcagcttat ttttattggt ctctattatg gcatgcaatg tttttgcatt
     5761 ttccatttct ggtggtgtaa gtgagaggag ctataaagag actgaaaaaa catcagcgat
     5821 gacgaccaca cactctacaa aacttcagcc atcacaggcg attttgttta agatgagaga
     5881 agatgcgcca ccattaaacc tcacagaaga aatcacgcct acttatccga caaaggcgaa
     5941 ttatcttatt catcctgtgc gatagcgtct gtcacatggc aggtgaacaa tcatgtctga
     6001 tgctgatgtg aacacctgcc caaatgagcc atcctgaccc actatcgcct gccatacccc
     6061 accataaagc gaatcatcaa gccacaagcc ggggcaagaa acataaatat ccccgtcact
     6121 agttcattac cgccagttaa ccacatggtg aagtaataaa tgagtgcagc gaggatcacc
     6181 atgccgaaga aagaagagac tgccgatacc acgttcagta cctgaccgat gtagccgctt
     6241 acgcagacaa tacacagggc gagaaacatc cataaataaa tcatagctca acctcctgcc
     6301 gcgctaaaaa acctgtaact tcagtcactt taatagtaag acaatcctta acctctggca
     6361 atttttgcta agcatcaatg aaaacagcat gttaaatgca agactgttgt gtacggaaaa
     6421 atatttactt tgcacgatta ataatcagta gctgaaagca gtcagcgagt gataaagtac
     6481 agataccgca ttcatgctcc tcgtggttat gtcctgacta gtctttacac tctttacagg
     6541 aaccattgtc gtacatgatg gcccaaccaa ttgagtgtac cgctgtcgac atggaatctt
     6601 ctgcgataca acaattcgta tctacagaag gtaactatgt ttccacaatg caaattttcc
     6661 cgcgagtttc tacatcctcg ctactggctc acatggtttg ggcttggtgt actctggctt
     6721 tgggtacagc ttccttatcc tgttctctgc tttctcggca cgcgtattgg cgcaatggcg
     6781 cgaccattcc tgaaacgtcg tgaatctatc gcccgtaaaa acctggaact ttgtttcccg
     6841 cagcattctg cggaagaacg cgagaagatg attgccgaaa actttcgttc actcggcatg
     6901 gcgctggtag aaaccggcat ggcatggttc tggcccgaca gtcgcgtacg taaatggttt
     6961 gatgttgaag ggttggataa ccttaaacgc gcacaaatgc aaaatcgcgg cgtaatggtt
     7021 gtcggcgtcc attttatgtc gctggaactg ggcggccgcg tgatgggact gtgccaacca
     7081 atgatggcta cctatcgtcc acataataat cagctgatgg aatgggtgca gacccgtggg
     7141 cgcatgcgct ctaacaaagc gatgatcggc agaaataatc tgcgcggcat tgtcggtgca
     7201 ctgaagaaag gtgaagcggt atggtttgct cccgatcagg attatggtcg taaaggcagc
     7261 tccttcgcgc cgttctttgc ggtggaaaat gtcgccacaa ccaatggcac ctatgttctc
     7321 tcccgtctct ctggcgcagc catgttgacc gtaacgatgg taagaaaagc ggattacagc
     7381 ggatatcgtt tgttcatcaa cccagagatg gaaggctacc cgacagatga aaatcaagcc
     7441 gctgcctata tgaacaagat tatcgagaaa gagatcatgc gcgcaccgga gcagtacctc
     7501 tggatccacc gtcgctttaa aacgcgcccg gtgggagaat cgtcgttgta catttaacaa
     7561 actgctctta ctctttccct cctcaaattg gccataataa cctcatggtt tttatggctt
     7621 ttttcttaag gttttttcct tcgctgaata tctaattatt actgtcagaa attaaactgt
     7681 cgccgatatg agacacttgt aagttactaa aagtaaataa gaaaatgcct cttgtctcgt
     7741 ttcagtttta ggcgtacatt agcgccgtct cgagcagttt agctcagaat tctggggtga
     7801 tgcccacggt aaaatacagg gcttttaagg tcgctttgcc cggtgtcaac tcactgtatc
     7861 aggtgtaatg aagtcattca ggcgtaacag taattacgcg gagagatgta aagtgaaata
     7921 tttctttatg ggcatttctt ttatggtcat cgtttgggcc ggtacttttg ccctgatgat
     7981 ctaaaagcaa actgtgcaat gaaaaacagg agccatcagg ctcctgtttg catttctgac
     8041 gacggtgttt attccgcgtt ttcgtgaata tgtttgctgc tggcgggtag taaaccgtcg
     8101 gcacggaaca tcgctttaat ccctctgatg gcctgacgaa tacggtcgcg gttttcaatc
     8161 agtgcaaagc gaacatgcgt atcaccatag tcgccaaagc caatccccgg cgagacacaa
     8221 accttcgctt cgttaaggag cttcttggca aattccagcg atcccatggc cgcatatggt
     8281 tccgggattt tcgcccagac atacatcgaa gccttcggca tttcgaccat ccagcccgct
     8341 tcatgcagcc ctttaaccaa tacatcacgg cggcgtttgt actgttcggc aatgtcgcgc
     8401 acgcactgtt gatcgccctc gagtgccgca atcgccgcca cctgcaacgg cgtaaaagtg
     8461 ccgtaatcgt gatagctttt gattcgcgcc agggcactga ccagcgtttt gttgccgacc
     8521 ataaagccga tacgccagcc cgccatgttg tagcttttcg acagcgtaaa gaactcgact
     8581 gccacatcgc gtgcacccgg tacctgcata attgacggcg ctttccagcc atcgtagacg
     8641 atatcggcat aggccaggtc atggaccacc agcacatcgt agcgtttcgc cagcgccact
     8701 actttttcga agaactccag ttccacgcat tgcgcggttg ggtttgacgg gaagccgagg
     8761 atcatcatct tcggtttcgg ataactttca cgaatggcgc gctccagttc gttgaagaaa
     8821 tcgacacctt ccaccagcgg cactgagcgt acctgcgccc cggcaatcac cgcgccataa
     8881 atatggatcg ggtaacttgg attcggcacc agcaccgtgt caccatgatc cagcgtcgcc
     8941 agcatcagat gcgccaggcc ctctttcgaa ccaatagtga cgatggcttc tgattccggg
     9001 tcgatttcaa cgtcgtagcg atcctgatac cagcgggaaa tggcgcgacg taaccgcgga
     9061 atgccgcgtg aagtggagta accatgcgtg tccgggcgct gggccacagt acataatttt
     9121 tcgacgatat gcggcggagt cgcaccgtcc gggttaccca tgctgaaatc gataatatct
     9181 tcgccgcgcc gacgcgcagc cattttcagt tcagcggtga tgttaaaaac gtagggcggg
     9241 agacgatcaa tgcgcgtaaa gcgacgttca gggcgagtgt cagccataat ttcctcagat
     9301 taacgttagc gcccggaccg tccgagcgac gctgccacaa tggtggctgt tttgaaaata
     9361 gcctgattaa tttctgtctg tccagcgttt tagaacattt ttatcaatgg ctttaaatga
     9421 ggaactccac ttcaaaaaca ggaaattttt ctacgctaac aagaaaatta ccaccaattt
     9481 tattaacatt ataatttcaa taaatttacc agccagaagt gcattatcaa gagatacatt
     9541 gctaatacac catttatctt tcaatcccct tttaaattta tggtttttcc tcaaaaacgg
     9601 cctgctggtc atcagccttc aggttaccta tcatagaggt ttaatcctta ttcagagtca
     9661 cccgtgcacg aaatattcaa catgctgctg gcggtcttcg atcgggcagc gttaatgctt
     9721 atctgcctgt tctttctcat ccgtatccgc ctgtttcgcg aactgttgca caagtcggcg
     9781 cactccccaa aagaattgct cgccgttacc gccattttct cgctgttcgc cctgttcagc
     9841 acctggtccg gcgttcccgt agaaggctcg ctggtgaacg tacgtattat cgcggtgatg
     9901 tccggcggga ttctgtttgg cccgtgggta ggcatcatta ccggcgtgat tgcgggtatt
     9961 caccggtatt taattgatat cggcggcgtg acggcgatcc cctgctttat caccagcatt
    10021 ctggcgggtt gtatatcagg ctggataaac ctgaaaatcc ccaaagcaca gcgctggcgc
    10081 gtcggtattc tcggcggcat gttgtgtgag acgttgacca tgattctggt aattgtctgg
    10141 gcaccaacta ccgcgttggg gatcgatatc gtctctaaaa tcggcattcc aatgatcctc
    10201 ggtagcgtct gtatcggctt tattgtgctt ctggtgcaaa gcgttgaggg cgaaaaagag
    10261 gccagcgccg cgcggcaggc caagctggcg ctggatatcg ccaacaaaac gctacctctg
    10321 tttcgccatg tcaatagcga gtcattacgc aaagtttgtg aaattattcg cgatgacatt
    10381 cacgccgatg cggtggcgat taccaatacc gatcatgtgc tggcctatgt tggcgtgggt
    10441 gaacataact atcagaatgg cgatgacttc attagcccga ctacccgtca ggcgatgaat
    10501 tacggaaaaa tcatcattaa aaacaatgat gaagcccacc gcacaccaga gattcattcc
    10561 atgctggtga tcccattgtg ggagaaaggc gttgtgaccg gaacgctgaa aatttactac
    10621 tgccacgcgc atcagatcac ctcgtcatta caggaaatgg cggtcggtct gtcgcaaatc
    10681 atctccaccc aactggaggt ttcacgcgcc gagcagctac gtgaaatggc aaataaggca
    10741 gagcttcgcg ccctgcaaag caaaattaat ccccatttcc tgtttaacgc tttgaacgct
    10801 atttcatcat caatccgtct gaatccggat actgctcgcc agctgatctt taatctgtcg
    10861 cgttatctgc gctataacat tgaattaaaa gacgatgagc aaatcgatat caaaaaagag
    10921 ctgtatcaaa ttaaagacta tattgccatt gagcaggccc gctttggcga caagctgacg
    10981 gttatctatg atattgatga agaggtgaat tgctgcattc ccagcctgct gatccagccg
    11041 ttggtggaga atgccattgt ccacggtatt cagccttgca aaggtaaagg cgttgtcacc
    11101 atcagcgttg cagagtgcgg caatcgggta cgcattgcgg tgcgggatac cgggcacggc
    11161 atcgatccaa aggtgattga gcgggtcgaa gcgaatgaaa tgccgggcaa taaaattggc
    11221 ctgctgaatg ttcatcatcg cgtgaagtta ttgtatggcg aggggctgca tatccgccgc
    11281 ctggaaccag gaacggaaat tgcgttttac attcctaacc aacgcacccc agtcgcctca
    11341 caggctacgt tattgctttg agccggagtg atattgtgaa agtcatcatt gttgaagacg
    11401 aattcctggc acaacaggaa ctgagctggc taattaaaga gcacagccag atggagattg
    11461 tcggcacctt tgacgacggt ctggacgtgt tgaagttttt gcagcataac cgcgtcgacg
    11521 ccatttttct ggatatcaat attccgtcgc tggatggcgt gttgctggcg caaaacatca
    11581 gccagttcgc ccataaaccg tttattgtgt tcatcaccgc gtggaaagaa catgcggtag
    11641 aagcgtttga actggaggcg tttgactaca ttctcaaacc gtatcaggag tcacgtatta
    11701 ccgggatgct gcaaaaactg gaagcggcct ggcaacaaca gcagaccagc agtacgcctg
    11761 ccgcgacggt aacgcgtgag aatgacacca ttaatctggt gaaagatgag cgaataatcg
    11821 tcacgccaat taacgatatc tattacgccg aagcgcacga gaaaatgacc tttgtctata
    11881 cgcggcgtga atcctacgta atgccgatga acattaccga attttgcagc aaactgccgc
    11941 cgtcgcattt tttccgctgc catcgctcat tttgtgtcaa tctgaacaaa atacgcgaaa
    12001 tcgaaccgtg gtttaataac acctacattc tgcgactgaa agatctggat tttgaagtgc
    12061 cggtcagccg cagcaaagtg aaagaatttc gccagttaat gcatctttaa tcgggaactt
    12121 tcatgaaagc accaggcttg cccgccgatc aacaattttt cgccgatctg ttcagcggcc
    12181 tggtgcttaa cccgcaacta ctggggcgcg tctggtttgc cagccagcct gcctcgttac
    12241 cggtgggcag tttatgtatt gattttcccc gtctggatat cgtgctgcgc ggcgaatacg
    12301 gcaacctgct ggaagcaaag cagcagcgaa tggtggaagg agaaatgctg tttattccgg
    12361 cacgcgcggc taatttaccg atcaacaaca aaccggtgat gctgttaagc ctggtgttcg
    12421 caccgacctg gcttgggtta tcgttttacg atagccgcac cacgtcgttg ttgcatcctg
    12481 ctcgccagat ccagcttccc agcctgcaac gcggtgaagg tgaagcgatg cttaccgccc
    12541 tcacccatct tagccgttcg ccgctggagc agaatatcat tcagccactg gtgttaagtt
    12601 tgctgcatct ttgccgtaac gtggtgaata tgccgccggg caattcgcag ccgcgcggcg
    12661 attttctcta tcacagcatt tgtaactggg ttcaggataa ttatgcccag ccgctcaccc
    12721 gcgagagcgt ggcgcagttt tttaatatca cgcccaatca tctgtcaaaa ctgtttgctc
    12781 agcatggaac gatgcgtttt atcgagtatg tgcgttgggt gcgaatggcg aaggcgagga
    12841 tgattttgca gaaatatcat ctgtcaattc atgaagtggc acagcgttgc ggttttccgg
    12901 atagcgacta tttttgtcgc gttttccggc gtcagtttgg tctgacgccg ggagagtaca
    12961 gcgcccgttt tcagggctaa cgtcagaagg ttaattctgt ttccagcagc gtcaggatac
    13021 tttgtgcatc ttgcgcggca aacaacgact ggcggaagtt tttattcacc agtttacgcg
    13081 ccagctgcga gaagactttc acatgattca tcccttcgtt agcacccagc gtcagcatga
    13141 tcaccagttc gacttcgccc atttctgact gccagtcaat cggtttcgcc agccgggcaa
    13201 tgctgatact ggaatgacgg atccactgag atttggtgtg cgggatcgct acgccaaaac
    13261 caacgccggt ggtaacaatc tcttcccgct gccagacatc ttcttccagc tcaaacggat
    13321 gttcagtgcg cccgttaacg ccgaggttgc cgcacaggaa ctggatcgcc tgctctttat
    13381 tgctaaaatc ctgatcaaca aagatattct ccagcgccag cagtgggcga acgtcttctt
    13441 ccggcgtaaa ggcggtgagt aacgcttcaa tttcctgcgc actgcggcat tcacatgcct
    13501 gacgcgccag ttcccgacac gcctcgctat ccagttgacg aagctggctt ttcaccgccg
    13561 gaatacgcgg gctactcata ctcagctcat ccaggcccag cccaagcagt agcggcagat
    13621 aacggctttc accgcccagt tcaccgcaaa tgcctaccca tttgccccgc tgatgcgcag
    13681 tggtaacaat ctgctgcaac atgcgcagga acgatggcgt aatcgggtta tacagcggcg
    13741 atacgcgcgg gttattacga tcgaccgcat acagatactg ggtcatatcg ttagaaccga
    13801 tactgaagaa atcgacctca tcgcagaagt ggtcgatgat gtaacacacc gacggcactt
    13861 ccaccatgat acccagagta atcgtctctg catgacgcag gccatcgcgc ttaagctcaa
    13921 cgatcgcttt ttggatctcg cccttcaccc ataagatctg atcgaggctg tgaaccatcg
    13981 ggatcatcaa ctgggcgttg ccaaaactgg cagcgcgcaa aatcgcccgt agttgagtgc
    14041 ggaacagggc agcaaattcc gggtaaatac gtaccgcgcg atagccgagg aacgggttct
    14101 cttcctgggg aatattcaga taaggaatgc ttttatcgcc tccgatgtcc attgtgcgga
    14161 agataatcgg cttgtcgccc gctgccagta gcacctgctg gtaggcttca aattgctcct
    14221 gctcgtccgg cgcgctgtca cgatccatat acagcatttc ggtacggaac agcccgacgc
    14281 cttccgcgcc gttggcaaac gcgcctggtg cttccagagc ggtgccaata ttcgccgcga
    14341 tatcaatacg cttgttatca cgggaatagg ccagctgcgc ggcagcctgc gcctgttgtt
    14401 tttggcgttt atccgccagc gtctgcgcaa cctgataata accgctcacc gcgtcattcg
    14461 ggttaatcgc cagcacgccg cactgggcgt caagcaccgc cggttgcccg gcataacggg
    14521 caatcgcatc cagcggcagg ccgctcagta ccgggatcgc cgaggcgcgg gccagaatca
    14581 gggtatgcga ggtgcgcccg gttttctcca gaatcatgcc cgcaagattt ttcaaatcaa
    14641 ggctcaaaaa ctggcttggg gttaaatctt cagccaccag aatggtcggt ttttccagca
    14701 ccagattgtt gcgcggcttc agttccggcc aggtgatatg cagcaactgt tcgctaatat
    14761 cgcgaatgtc gctaacacgt tcgcgcagat aatcgctggc agaagcagaa agcttggcgc
    14821 aaacctgctc catattgctg atgatcgccg cccccagtcc ctgatgctgt tctgtcatca
    14881 ggcgacggat attgcctgca aattcatcat cctgaatcag cgacaaatga gcgctgagga
    14941 tagttttgct ttcgccgtca cgctcacgca attgctggtt cagttgctcg gcaagcgttg
    15001 ccaggctgtg ctccagccgg gtggaatctt gcgcactggc ggggattgcc cgataactgt
    15061 cgaggctgtc gctttgtaac agggtcagcg tacccacgcc gacgccgctt gccagcacat
    15121 tgccgtacag taaatccggg ttaaggcggc ttaatgaacg cggcagcgga tgcgccgtca
    15181 gttctgcctg cgtaggctga acgctgtcgc tgtcgataaa gcgcacctgg atgtactctt
    15241 ccagcacgcg ccgcgcctgc tcttcatcgc tgccgctaat gttcaggctg caactgtcat
    15301 taaatagggt gccggtgcca atcagcgcca gcgaactttt cgcatcggcc tttgcgttct
    15361 ggcgatggtt aataaaggtg acttcacttt gccactggct gcactgttct ttaagttccc
    15421 acgccggacg agcatgcaga ccgttaggca aaggacagag aaattgaatc gttaacataa
    15481 ccgctccggg ttaatcagga gatcatctga aatccgtcag ttgaacaacc gtctcacggg
    15541 taagacgttg aataagggca gataaaagtt gctgcatctg caaaatgtcg cggcaatcgg
    15601 caatcgatgc cgcacaatgt ccgtggcgcg tcgctggccc catcaccacc gtgggcacgc
    15661 cggtgccggt taaatgcacc gctccgccgt ccgtgccgcc gttgctgaac atatccgcct
    15721 gcaacggcac gccaatttct gccgcgacgg tttcgatcca ggcggtaagt tttggcggcg
    15781 caatcagcga cttgtcgctt aacaccagca tcggcccgtt accaatctgg cgatggttag
    15841 ccgcgctata atcaaagttt ttcgcccagc aggcggtatc aagcacaatg gcgacatccg
    15901 gcgacaccgc gcgggtggca gtttgcccgc cgcgtaatcc cacctcttcg ctggaacttg
    15961 ccaccagcca cacctccgca ggcagttcgg cgtcgtgcag ctcacgtagt aacgtcacca
    16021 gcagatagca accgaggcgg tcatcaaagg ctttccccat cacccgctgg tgagggagaa
    16081 cctgaaaagt tgtatcaaag gtgacgcgat cgcctggacg aattcccgcc tgcatcactt
    16141 cgtcatacga gcgcgcacca atgtccacgc gcatggcgct gacgtcattc ccctgccggt
    16201 cgccgtcaag caggcctgga attttgcact cttcacgggt ggtgatgcgc accggctgca
    16261 gctggcgggc agccatgcgc acgttgccaa ccggcagcac gtcaatagcg ccttagcggg
    16321 agatgctgcg caccataaat cccacttcgt ccatatgggc gcagatcatc accttcggac
    16381 ctgtcgattc attgaggcgg atcagcaccg atcccagacc atcaaatcgc acttcttttt
    16441 gcaggcgatc cgcttcttcc agtaggatct gccgcacttc ctgttccgag gaggcgatcg
    16501 catctgcctc gctcaacgct tttaatagcg ataaatccat tatgcctctc ccgtgagcaa
    16561 cactgttttc ggcatggcgt agagcacttc tgcgccttgc ggggtaacca gcacaacatc
    16621 ttcgatgcgc acgccccctt gccctggcaa ataaatcccc ggctccacgg tcagcaacat
    16681 gcctggctgt agcgtcgtgg tgtcccgcgg tgaaaaacgc ggatcttcat ggacttcaat
    16741 gccgatagcg tgaccagtgt tatgaccgaa atagtcgcca aatccagcct cggtaatgac
    16801 ccggcgcgcg gcgtcgtcaa tctgctggca gcgcacgccg gggcgaattg cagagattgc
    16861 cgcgagctgt gcctgcagga caatctgata gacgtcaaac agcgggtgag attcggcgct
    16921 caccccttcg ccattcacca gcaaggtgcg cgtcatatca gagcagtagc cctgatacag
    16981 cgcaccaaaa tcgagagtga caaactcgcc cgctgcaaca atcttgtcgc tggctttgcc
    17041 gtgcggcagc gccccgcgcc agccgctggc gacaatggtg tcaaaagagg ctttttctgc
    17101 gccctgctgg cgcataaacc actccagttc agcggctatc tcgcgctcgc tcatccccgc
    17161 ctgaataaag cggcgaatat gctccgctcc gcgatcggca atcccacagg cgaggcggat
    17221 tatctccacc tcctctggcg ttttgatttg ccgcagcaca tccggcgtgg cgctaaccag
    17281 tttcgcattg agttcagact tccagcggtg cgcggtttcc caactcacct gctggccttc
    17341 aaaaccgagc gtttgcaact gctcatcggc aatgatttga ttgacgatag tggtaagcgt
    17401 gttcgtcgcg tcaagcaaat gcagctggta gccttgcgca cgggcttcca cctcaacgaa
    17461 atagcgcgaa tctaccagaa tgtgcgcact ttcacggcta ataaccacat agcctgatcc
    17521 ggtggagatc cccagatgcg gctgtttgtt ctgccgtgag gagagaagca ctgcatccag
    17581 ttgttgcgcc ttaagccagt cgcgcagcga agcgagtaat gtcatctgga ttttccttct
    17641 tacaggctat cgattaacaa tttgccttta cggaacatca tcagacgcag gaacaccacc
    17701 atcagcgcag ttatgactgc ccccagcgcg atccccgcca tatagacgcc caggttagtg
    17761 accagcggcc atgcccagat agcggattcc gggaaccatt gcactgcgcc cagccagaca
    17821 gcggcggttg agccgacaat cgcgccgacc atatacgacg gaatggcggt gatggggctt
    17881 tccagcgcaa acggaatcgc accctcacta atccccataa aggcaaggaa catcgcggtc
    17941 ttaccctgcg gataaagttg agcgttgaac aggcgtttgc cggttaaacg acggtcaata
    18001 atggtcgcca gacccagacc aatcggagga ataacgatag cgatagaacg agcggtgacc
    18061 ggtaatacgt ggtcggtggt aaagctgaag gcaacgaaac ctgccgcttt gttgattggg
    18121 ccaccaaggt cgattgcggt cgccgcggca atccccattg catacatcag cgcgcccttc
    18181 tcacctgcgg cagtcagcac ggtacggata ccgccgttga tccagccacc aaatggggtg
    18241 atgacatagt acatcgccag catgacaaaa atggcggaaa gaatcggtag caaaaatgtg
    18301 gttttgaacg ctaacaggaa atctggtaac tggattttct ggttcatcca tttcacaagg
    18361 taaccagcaa cgatagatat aatcagcgca ccaatgaagg tggacggcac cggcgaagag
    18421 gtcgcccatt gcatagtgct gggatcgaag ttcagcagtt gcgtcggctg ggtagacatc
    18481 aagccgccga taaaaccagc cgggaacgcc agtttgccgc cgatagagtt cgccacaaaa
    18541 gcggcaaaca tcggaatagc aaagccaaac aacacgccgc cgaaggactg cgacagccag
    18601 gcaaatttca gtaaggagag gtcgaaaccg gagaatttcc cgctattaag agcatccatg
    18661 atgccgatct cagcaggaat tttaagccag ctataagcaa tcagctggct gaaagcgagg
    18721 atcacaccgc ccataattaa ggtcggcacc atgcgtgaaa tccctgacat cacatgctgc
    18781 gggagttcac cccagaagct gcttttagag gcctgcggat tcttaactgc cgctgccgca
    18841 ccggatgcgc caggcacaac ggttgcactg cgttttttaa tggccataac aattatccct
    18901 gtcgaatatt actgttgttc agaggcaatc atctcttcga tttctttgat gatgcctaca
    18961 gcgtttttaa ttgcgtcctg caaagtgatt tcataaacgt cgcgtgattc gaaacgttcg
    19021 ttatcttccg gggtaactgc cacggaatgg atgatgatgg tcgcttcggc gatatcctgc
    19081 gccgtcaggc gattctggat gccgtccgcg ccctgggttt caattttcac ttcataaccg
    19141 gcttctaccg ccgcttcttc cagcgcctga gcggccataa aagtgtgagc caggcccatc
    19201 gggcaggcac ataaggcaat cagtttctta ctcatcgtta attcctcgtt cagtgatagt
    19261 tgacgagtga aattatgaac gtgcaaaaca aatcgccgtt accagacaaa taatgcattt
    19321 actggagaat taatcccgct gtatggaagt gtgacgcagg tcggccttgt gaggggacac
    19381 aaagaaaaat tgaattaact gtatgattta aaagattatc gggagagtta cctcccgata
    19441 taacaggaag gatttacaga atgtgaccta aggtctggcg taaatgtgca ccggaaccga
    19501 gaagacccgg attgtcatgg acgatgagat acaccggaat atcatggaca tattctttaa
    19561 agcgcccttt atcttcaaat gcggcacgga aaccagaggc tttgaagaac tcaaggaagc
    19621 gcggcacgat accgcccgca ataaacacgc cgccaaatgt cccgagattg agcgccagat
    19681 tgccgccaaa acggcccata atgacgcaaa acagcgacaa cgcgcggcgg caatcggtgc
    19741 agctatcagc cagcgcacgt tcggtaatat cttttggctt gagattttct ggcaggcggt
    19801 tgtcagcttt cacaattgcg cgatacaaat tcaccagccc agggccagaa agcacgcgtt
    19861 ccgccgaaac atggccaatt tccgcacgca gtatttcgag gataatggcc tcttcttcac
    19921 tattcggcgc aaaatcaacg tgaccgcctt cgcccggcaa gcttacccaa cgcttatcga
    19981 catggaccag atgcgcaacc ccaagccccg ttccggcacc gtaaaccgca ataggcttac
    20041 cttctaccgg ttctgcgcca ccaaactgaa tcagatgctc ttttttcagc atcgggatcg
    20101 ccatcgatac agcggtaaaa tcgttaataa tttccagatg gctaaaaccg agattctttt
    20161 tcatttcggc aattgagaac gcccaggtat ggttggtcat tgccacccag tcaccggtaa
    20221 ttgggcaagc gatggcaata cagccgtctt tcacctcgac cttatgttct tcaagataaa
    20281 cgcgaatgac cgcttcgagg ctggggtaat caagccctga ataggtctta gcctgcgaga
    20341 tttcaccact ggcaatatca cacagagcaa gacgtgcgtt ggtgccgccc acatcaccga
    20401 ctaatgcata ctttgtcatt cttcaactgc tccgctaaag tcaaaataat tctttctcac
    20461 actgtaaata cctgggggca taacaacaac gccggaaagg caggctccct gtaaatatcg
    20521 atctgggtca cacatttact ttatcgtttc agcaccaatt gcagcgatgc ctttttgcaa
    20581 gctgggcaaa ctaagtatct gaccccgcat aaggaataga acatgctcca tccgcgagcc
    20641 agaaccatgt tgttattatc gctccccgcc gtggcaattg ggattgcgtc cagtcttatt
    20701 ctgattgtgg tgatgaaaat cgcctcggca ttacagaatt tgctctggca acgactgccg
    20761 ggaactctgg ggatagccca ggattcacct ctctggatca tcggtgtatt aacgctaacg
    20821 ggtattgcgg tggggttggt tatccgtttc agccagggtc atgccggacc agaccccgcc
    20881 tgtgaaccgc tgatcggcgc accagttccg ccctctgcgc taccaggact tatcgtagca
    20941 ttaattctcg gtcttgctgg cggcgtcagc ctggggccgg aacatccgat catgaccgtc
    21001 aatatcgccc ttgcggttgc cattggcgct cgtctgttac cgcgcgtcaa ccgaatggag
    21061 tggactattt tagcctctgc cggaaccatc ggcgcgctgt ttggtacacc tgttgcggcg
    21121 gcgttgatat tttcgcaaac cttaaatggc agtagtgaag ttccgctatg ggatcgtctt
    21181 tttgcgccat taatggcggc agcagctggt gcacttacta ccggattatt tttccatccc
    21241 catttttcac tgcccattgc gcattacgga cagatggaaa tgaccgatat tctcagcggt
    21301 gcaattgtcg cggcgattgc catcgcagca gggatggtcg ccgtatggtg cttaccacgg
    21361 ttgcacgcga tgatgaatca aatgaaaaat ccggtgctcg tgctgggtat tggcggattt
    21421 attctcggta ttctgggggt tattggtgga ccagtttcgc tgtttaaagg gctggatgag
    21481 atgcagcaga tggtggcaaa tcaggctttc agcaccagcg attacttttt gctggcggta
    21541 attaaacttg ccgccctggt cgttgctgcc gccagtggct ttcgcggtgg gcgaatcttc
    21601 ccggcagtgt ttgtcggcgt ggcattaggg ttgatgctgc atgagcacgt acccgccgta
    21661 ccagcggcaa taaccgtttc ttgcgctatt ctcggcatcg tgctggtggt aacacgcgat
    21721 ggctggttaa gtctttttat ggcggcggtc gttgtaccca ataccacatt gctaccgctg
    21781 ctctgtatcg tcatgcttcc ggcatggctg ttattagcag gtaagccgat gatgatggtc
    21841 aatcgtccga agcaacagcc accccacgat aacgtttagc aaaaaatgct ctcctttatg
    21901 atttaagagt tatggcgatg atacggggct ttacctcccc gtaatattgc gttaacaggc
    21961 cgctgacggc ttcgatcgtg aaggagaata acaatgttca ggtcactgtt tctggcggcc
    22021 gccctgatgg catttacccc gcttgcagca aacgcaggtg aaatcaccct actgccatca
    22081 atcaaattac aaattggcga tcgcgatcat tacggtaatt actgggacgg tggtcactgg
    22141 cgcgaccgtg actactggca tcgcaattat gagtggcgta aaaaccgctg gtggcgtcat
    22201 gataatggct atcatcgtgg ctgggataag cgtaaagcgt atgagcgtgg ctatcgtgaa
    22261 ggctggcgcg atcgtgacga tcatcgcgga aaaggccgcg gacatggaca ccgccattaa
    22321 acacattcgc caatggaaca caatgcctga tgcgacgcta gcgagtctta tcaggcctac
    22381 aaaccggctc tttaacgcgt agggcggata aggcgtttac gccgcatccg ccaatagtgc
    22441 cagatgcgac gctcattcaa ctacaatccc agcgccgtcc ccaccaacaa ccagatattc
    22501 agcgccacga ccagcactac aatcacccag cctgtctgtt ttacgcgttt gctgttcacc
    22561 agatcgccca tcaacttgct gtcactggtg aaaatcagca gtggaaccag cgccagggcg
    22621 ataccaaaac ttaacagcac ctgactcata accagaatcc gtgtcggatc taatcccatc
    22681 agaatgacaa taaatgacgg caacatggtg actgtacgac gcacccacag cggga
//
LOCUS       DAZGAN020000072        22440 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGAN020000072 DAZGAN020000000
VERSION     DAZGAN020000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.072"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            273..956
                     /locus_tag="ACHKYO_RS15500"
                     /old_locus_tag="pgaptmp_003100"
     CDS             273..956
                     /locus_tag="ACHKYO_RS15500"
                     /old_locus_tag="pgaptmp_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU4429912.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1000..5850
                     /gene="ehaG"
                     /locus_tag="ACHKYO_RS15505"
                     /old_locus_tag="pgaptmp_003101"
     CDS             1000..5850
                     /gene="ehaG"
                     /locus_tag="ACHKYO_RS15505"
                     /old_locus_tag="pgaptmp_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HGU4429913.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVSNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6218..7873
                     /gene="lldP"
                     /locus_tag="ACHKYO_RS15510"
                     /old_locus_tag="pgaptmp_003102"
     CDS             6218..7873
                     /gene="lldP"
                     /locus_tag="ACHKYO_RS15510"
                     /old_locus_tag="pgaptmp_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HGU4429914.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7873..8649
                     /gene="lldR"
                     /locus_tag="ACHKYO_RS15515"
                     /old_locus_tag="pgaptmp_003103"
     CDS             7873..8649
                     /gene="lldR"
                     /locus_tag="ACHKYO_RS15515"
                     /old_locus_tag="pgaptmp_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HGU4429915.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8646..9836
                     /gene="lldD"
                     /locus_tag="ACHKYO_RS15520"
                     /old_locus_tag="pgaptmp_003104"
     CDS             8646..9836
                     /gene="lldD"
                     /locus_tag="ACHKYO_RS15520"
                     /old_locus_tag="pgaptmp_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HGU4429916.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKEFPAALAPMAK
                     GNAA"
     gene            9991..10464
                     /gene="trmL"
                     /locus_tag="ACHKYO_RS15525"
                     /old_locus_tag="pgaptmp_003105"
     CDS             9991..10464
                     /gene="trmL"
                     /locus_tag="ACHKYO_RS15525"
                     /old_locus_tag="pgaptmp_003105"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HGU4429917.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10517..11338)
                     /gene="cysE"
                     /locus_tag="ACHKYO_RS15530"
                     /old_locus_tag="pgaptmp_003106"
     CDS             complement(10517..11338)
                     /gene="cysE"
                     /locus_tag="ACHKYO_RS15530"
                     /old_locus_tag="pgaptmp_003106"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGU4429918.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11418..12437)
                     /gene="gpsA"
                     /locus_tag="ACHKYO_RS15535"
                     /old_locus_tag="pgaptmp_003107"
     CDS             complement(11418..12437)
                     /gene="gpsA"
                     /locus_tag="ACHKYO_RS15535"
                     /old_locus_tag="pgaptmp_003107"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGU4429919.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPPMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12437..12904)
                     /gene="secB"
                     /locus_tag="ACHKYO_RS15540"
                     /old_locus_tag="pgaptmp_003108"
     CDS             complement(12437..12904)
                     /gene="secB"
                     /locus_tag="ACHKYO_RS15540"
                     /old_locus_tag="pgaptmp_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGU4429920.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12967..13218)
                     /gene="grxC"
                     /locus_tag="ACHKYO_RS15545"
                     /old_locus_tag="pgaptmp_003109"
     CDS             complement(12967..13218)
                     /gene="grxC"
                     /locus_tag="ACHKYO_RS15545"
                     /old_locus_tag="pgaptmp_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HGU4429921.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13360..13791)
                     /locus_tag="ACHKYO_RS15550"
                     /old_locus_tag="pgaptmp_003110"
     CDS             complement(13360..13791)
                     /locus_tag="ACHKYO_RS15550"
                     /old_locus_tag="pgaptmp_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGU4429922.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVISRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14036..15580
                     /gene="gpmM"
                     /locus_tag="ACHKYO_RS15555"
                     /old_locus_tag="pgaptmp_003111"
                     /gene_synonym="pgmI"
     CDS             14036..15580
                     /gene="gpmM"
                     /locus_tag="ACHKYO_RS15555"
                     /old_locus_tag="pgaptmp_003111"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HGU4429923.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15614..16873
                     /gene="envC"
                     /locus_tag="ACHKYO_RS15560"
                     /old_locus_tag="pgaptmp_003112"
     CDS             15614..16873
                     /gene="envC"
                     /locus_tag="ACHKYO_RS15560"
                     /old_locus_tag="pgaptmp_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HGU4429924.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16877..17836
                     /locus_tag="ACHKYO_RS15565"
                     /old_locus_tag="pgaptmp_003113"
     CDS             16877..17836
                     /locus_tag="ACHKYO_RS15565"
                     /old_locus_tag="pgaptmp_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HGU4429925.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17823..18857)
                     /gene="waaH"
                     /locus_tag="ACHKYO_RS15570"
                     /old_locus_tag="pgaptmp_003114"
     CDS             complement(17823..18857)
                     /gene="waaH"
                     /locus_tag="ACHKYO_RS15570"
                     /old_locus_tag="pgaptmp_003114"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HGU4429926.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(19096..20121)
                     /gene="tdh"
                     /locus_tag="ACHKYO_RS15575"
                     /old_locus_tag="pgaptmp_003115"
     CDS             complement(19096..20121)
                     /gene="tdh"
                     /locus_tag="ACHKYO_RS15575"
                     /old_locus_tag="pgaptmp_003115"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HGU4429927.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20131..21327)
                     /gene="kbl"
                     /locus_tag="ACHKYO_RS15580"
                     /old_locus_tag="pgaptmp_003116"
     CDS             complement(20131..21327)
                     /gene="kbl"
                     /locus_tag="ACHKYO_RS15580"
                     /old_locus_tag="pgaptmp_003116"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HGU4429928.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPYLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21602..22438)
                     /gene="yibB"
                     /locus_tag="ACHKYO_RS15585"
                     /old_locus_tag="pgaptmp_003117"
     CDS             complement(21602..22438)
                     /gene="yibB"
                     /locus_tag="ACHKYO_RS15585"
                     /old_locus_tag="pgaptmp_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HGU4429929.1"
                     /translation="MTAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALENEMI
                     IFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQLKN
                     PEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFD
                     ESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKIT
                     LNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLS
                     RK"
BASE COUNT         5731 a         5763 c         5674 g         5272 t
ORIGIN      
        1 tacaataaat tatctgtaaa ttttttccca gacatggttt ctttcatatt tgacatttat
       61 taataatcat cattagaatc cacgaaaata atatattcat acaaacacag acatcttttg
      121 aaacgaatga tacattttta tgatgaatgc ttaaaacaat atttaatcga cacacatcac
      181 tgtcattaat gtaaagcaac atagatcacc aataatacaa tggacaactc taccggcatt
      241 gatcgctggt cggaggaaat aaggattttt acatgaactt gaagaagatt tttttcagtg
      301 ctgtcactgt ttcagttctc tgcgcattaa cgggatgtga ctacattgaa gaaggcaagc
      361 cagaaagtag tttattgaaa caacaagaag aacataacaa taaaattgat ctacttgaaa
      421 aacaacaagc acagctaaag agtcaactgg aaactattca aaaacaacaa actggcataa
      481 taagcagtac aaagacttta actcacgtaa ttaaatccgt taaagatcaa caaaacactt
      541 ttatttttac cgagtttaac ccagcaaaaa cgaagtattt catcctgaac aatggttcgg
      601 tggctttagc gggtcgagtg ttatccattg acgccacaga gaatggcagt gttattcata
      661 tttcactggt caacttatta agtacaccta tctcaaatat tggttttaat gcgacatggg
      721 gtggtgaaaa acctgtcgat gccaaagagt ttgcccggtg gcaacaatta cttttcaaca
      781 catcaatgaa atccacattg aaattattac caggtcaatg gcaagacatt aatttgaccc
      841 tgaagggtgt atcgcccaat aacctgggat atctgaagtt agccatcaac atggaaaata
      901 ttcagttcga caatcttccc tctgctgaaa atcggcagaa aagaagcaaa aaataaatgc
      961 agttagtacg cttataacaa ggaatagcga gcatcattaa tgaacaaaat atttaaagtt
     1021 atctggaacc ctgcgacagg gaattatact gttaccagcg aaacggcaaa aagccgtggc
     1081 aagaaatctg ggcgcagtaa gctgttaatt tctgcgctgg ttgcgggtgg aatgttgtcg
     1141 tcgtttgggg cattggcgaa tgccgggaat gacaacggtc agggtgttga ttacggtagt
     1201 ggatcagctg gcgacggctg ggttgctata ggcaaagggg cgaaagcaaa tacttttatg
     1261 aacaccagtg gttccagtac tgctgtgggt tatgacgcta tagctgaagg ccaatatagc
     1321 tctgccatcg ggtcaaaaac ccatgcgatt ggcggtgcat caatggcctt tggggttagt
     1381 gcaatatcag aaggcgatag aagtatagca ctgggtgcct cttcgtattc attgggccaa
     1441 tactcaatgg ccctcggccg ttattcaaaa gcattgggta aattgtctat tgctatgggg
     1501 gactcttcca aagcggaagg agcaaacgcc attgccctgg gaaatgccac taaagctact
     1561 gagattatga gtattgctct tggcgacacc gccaatgcgt caaaagcgta ttcaatggcg
     1621 ctgggagcaa gtagcgtcgc atctgaagaa aacgcaattg ccctggggcg tagcagtgta
     1681 gctagcggta ctgacagcct cgcatttggc agacaatcac ttgccagcgc agcgaacgct
     1741 attgcgatag gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg caataatgcg
     1801 aaggcaaaag ggactaatag catggcaatg gggttcggaa gccttgccga taaagtcaat
     1861 actatcgcat taggaaatgg cagccaggct ctggcagata atgcaatcgc cataggccag
     1921 ggcaacaaag ctgatggcgt ggatgccatc gctctgggta atggtagcca gtcgagaggc
     1981 ttaaacacca ttgccttagg cacagccagt aatgcaactg gtgataagag tcttgcgctt
     2041 ggtagtaata gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga ttccattgcg
     2101 gatttagaca ataccgtctc tgtcagcaat agttcattaa aacgcaagat cgttaatgtg
     2161 aaaaatggcg cgatcaagtc tgacagttac gatgccatta atggttcaca gctttatgcc
     2221 attagcgact cggtagcaaa aaggcttgga ggaggggctg cagtagatgt tgatgacggt
     2281 actgttacag caccaaccta caatttaaaa aatggtagca aaaataacgt aggggctgcg
     2341 ctcgctgtac ttgatgaaaa caccctgcaa tgggaccaaa ccaaaggcaa atacagcgct
     2401 gctcatggta ctagtagccc aactgccagc gtaatcaccg atgttgcgga tggcacgatt
     2461 tcagcctcca gtaaggatgc ggttaacggt tcccaactga aagctaccaa tgacgatgtc
     2521 gaagccaaca ccgccaatat cgctactaat accagcaaca ttgccacgaa tacggcaaat
     2581 attgccacca ataccaccaa tatcaccaac ctgacggatt ccgttggtga ccttcaggct
     2641 gatgccctgc tctggaacga aactaaaaag gcattcagtg cagctcacgg ccaggatacc
     2701 accagcaaaa tcaccaacgt taaagatgcc gacctgacgg ctgacagcac tgatgctgtt
     2761 aacggctctc agctgaaaac caccaacgat gctgtggcga cgaataccac caatatcgcc
     2821 aataacactt ccaatattgc cactaacacc accaacatct ctaacctgac tgagacggtg
     2881 actaatcttg gtgaggatgc gctgaaatgg gataaggaca atggtgtatt cacggcagct
     2941 catggcaccg agaccaccag caaaatcacc aacgttaaag atggcgacct gacgactggc
     3001 agcaccgatg ccgttaacgg ctctcagctg aaaaccacca acgatgccgt ggcgacgaat
     3061 accaccaata tcgccactaa caccaccaac atctctaatc tgactgagac ggtgactaat
     3121 cttggtgagg atgcgctgaa atgggataag gacaatggtg tcttcactgc agctcatggc
     3181 aacaataccg ccagcaaaat caccaatatc ctggacggca cagtcactgc aaccagttcc
     3241 gatgccatta acggtagcca gctttatgac ttaagcagca atatcgccac ctacttcggc
     3301 ggcaatgctt ctgtgaatac tgacggtgtg tttaccggtc caacctacaa aatcggtgaa
     3361 acaaattatt ataacgtcgg cgatgcactg gctgcgatta actcctcatt tagcacgtct
     3421 ctcggcgatg ctctgctttg ggatgccacc gcaggtaaat tcagtgccaa acacggtact
     3481 aatggtgacg caagcgtgat cactgatgtc gcagatggtg aaatttcaga ctccagttct
     3541 gacgcagtaa acggctcaca actccacggc gtgagcagtt atgttgttga tgcgctgggg
     3601 ggtggtgccg aagtcaatgc agacggcacc atcactgcgc cgacgtacac cattgctaat
     3661 gctgattacg ataatgtcgg tgatgccctg aatgctatcg ataccactct tgacgacgct
     3721 ctgctctggg atgcggacgc cggtgaaaat ggtgcattta gcgccgctca cggaaaagat
     3781 aaaactgcca gtgtaatcac taacgtcgct aacggtgcaa tctctgctgc cagcagcgac
     3841 gcgattaacg gctcacaact ctataccacc aataagtaca tcgctgatgc gctgggtggt
     3901 gacgcagaag tcaacgctga cggcaccatc accgcaccga cttacaccat tgcgaacgcc
     3961 gagtacaaca acgtcggtga cgccctggat gcgcttgatg ataacgccct gctgtgggat
     4021 gagactgcca atggcggtgc tggagcctac aatgccagcc atgacggtaa agccagcatc
     4081 atcactaatg tcgctaatgg cagtattagt gaggacagta ccgatgcagt gaacggttct
     4141 cagttgaatg cgacgaatat gatgattgag cagaacaccc aaattatcaa tcagctcgct
     4201 ggtaacaccg acgcaaccta tatccaagaa aacggtgcgg gtattaacta tgtgcgtact
     4261 aacgacgacg gcttagcgtt caacgacgcc agcgcacagg gtgttggcgc tacagctata
     4321 ggttataact ctgtcgccaa aggcgatagc agcgtagcta ttggtcaggg cagctacagc
     4381 gacgttgata cgggtatcgc cctgggtagc agctctgttt ccagccgagt gattgccaaa
     4441 ggctcccgtg acaccagcat aacggaaaat ggcgttgtta ttggttacga caccacggat
     4501 ggcgaactgc tcggtgcatt gtctatcggt gatgacggta aatatcgtca aatcatcaac
     4561 gtagccgatg gttccgaagc ccatgacgcc gttacggttc gtcaattgca gaatgcgatt
     4621 ggtgcggtcg caaccacgcc gactaaatac ttccacgcta attcaacgga agaagattca
     4681 ctggcagtgg gaactgactc gctggcaatg ggtgcgaaaa ccatcgtgaa tggcgataaa
     4741 ggtattggta tcggttatgg tgcctacgtg gacgcgaatg cacttaacgg cattgccatt
     4801 ggtagcaatg cgcaagtcat tcatgtcaac agtattgcga taggtaatgg ttctacgacc
     4861 actcgtggcg ctcaaaccaa ttataccgcc tacaacatgg acgcaccgca gaactctgtc
     4921 ggtgaattct cagtcggtag tgcggatggt caacgtcaga tcactaacgt cgcagcaggt
     4981 tcggctgata ccgatgcggt caacgtgggt cagttgaaag taacggatgc gcaggtttcc
     5041 cagaataccc agagcattac taacctggat aatcgggtaa cgaatcttga ttcacgcgtc
     5101 accaatatcg aaaacggtat tggcgatatc gtcaccaccg gtagcaccaa gtacttcaag
     5161 accaataccg atggtgtaga tgccagcgcg cagggtaaag atagcgtcgc gattggttct
     5221 ggctccattg ctgccgcgga taacagcgtc gcactgggta cagggtctgt ggcaaccgaa
     5281 gaaaatacga tctctgtagg ctcatctact aatcaacgtc gtatcaccaa cgtagccgca
     5341 ggtaaaaacg acaccgatgc tgttaacgtg gcacagttga agtcttccga agctggcggt
     5401 gtgcgttacg acaccaaagc tgatggttct atcgactata gcaatatcac cctcggtggc
     5461 ggcaacggcg gtacgactcg tatcagcaac gtctccgctg gcgtcaacaa caacgacgcg
     5521 gtgaattacg cgcagttgaa gcaaagcgtg caggaaacga agcaatacac cgatcagcga
     5581 atggttgaga tggataacaa actgtctaaa actgaaagca agttgagcgg tggtatcgct
     5641 tctgcaatgg caatgaccgg tctgccgcag gcttatacac cgggtgccag catggcttct
     5701 attggtggcg gtacttacaa cggtgaatcg gcagttgctt taggtgtatc gatggtgagc
     5761 gccaatggtc gttgggtcta caaattacaa ggtagtacca atagccaggg tgaatactcc
     5821 gccgcactcg gtgccggtat tcagtggtaa tgatccatta agttagtgtg actaagggaa
     5881 aacgccaaat tgcctgatgc gttacgcgca tcaggcaaga taaggcgctc cgggtcaaca
     5941 atctaagggc aattctctga tgaggattgc ccttttcttt accagacatc tccccccaca
     6001 agaattggcc ctaccaattc ttcgcttatc tgacctctgg ttcacaattc cccaattaaa
     6061 actcacatca atattgccaa tacataacat ttagttaacc attcattgtc attatcccta
     6121 cacaacacaa ttggcagtgc cacttttaca caacgtgtga caaggagatg agcaacagac
     6181 tcattacacg atgtgcgtgg actccaggag acctgcaatg aatctctggc aacaaaacta
     6241 cgatcccgcc gggaatatct ggctttccag tctgatagca tcgcttccca tcctgttttt
     6301 cttctttgcg ctgattaagc tcaaactgaa aggatacgtc gccgcctcgt ggacggtggc
     6361 aatcgccctt gccgtggctt tgctgttcta taaaatgccg gtcgctaacg cgctggcctc
     6421 ggtggtttat ggtttcttct acgggttgtg gcccatcgcg tggatcatta ttgcagcggt
     6481 gttcgtctat aagatctcgg tgaaaaccgg gcagtttgac atcattcgct cgtctattct
     6541 ttcgataacc cctgaccagc gtctgcaaat gctgatcgtc ggtttctgtt tcggcgcgtt
     6601 ccttgaagga gccgcaggct ttggcgcacc ggtagcaatt accgccgcat tgctggtcgg
     6661 cctgggtttt aaaccgctgt acgccgccgg gctgtgcctg attgttaaca ccgcgccagt
     6721 ggcatttggt gcgatgggca ttccaatcct ggttgccgga caggtaacag gtatcgacag
     6781 ctttgagatt ggtcagatgg tggggcggca gctaccgttt atgaccatta tcgtgctgtt
     6841 ctggatcatg gcgattatgg acggctggcg cggtatcaaa gagacgtggc ctgcggtcgt
     6901 ggttgcgggc ggctcgtttg ccatcgctca gtaccttagc tctaacttca ttgggccgga
     6961 gctgccggac attatctctt cgctggtatc actgctctgc ctgacgctgt tcctcaaacg
     7021 ctggcagcca gtgcgtgtat tccgttttgg tgatttgggg gcgtcacagg ttgatatgac
     7081 gctggcccac accggttaca ctgcgggtca ggtgttacgt gcctggacac cgttcctgtt
     7141 cctgacagct accgtaacac tgtggagtat cccgccgttt aaagccctgt tcgcatcggg
     7201 tggcgcgctg tatgagtggg tgatcaatat tccggtgccg tacctcgata aactggttgc
     7261 ccgtatgccg ccagtggtca gcgaggctac agcctatgcc gccgtgttta agtttgactg
     7321 gttctctgcc accggcaccg ccattctgtt tgctgcactg ctctcgattg tctggctgaa
     7381 gatgaaaccg tctgacgcta tcagcacctt cggcagcacg ctgaaagaac tggctctgcc
     7441 catatactcc atcggtatgg tgctggcatt cgcctttatt tcgaactatt ccggactgtc
     7501 atcaacactg gcgctggcac tggcgcacac cggtcatgca ttcaccttct tctcgccgtt
     7561 cctcggctgg ctgggggtat tcctgaccgg gtcggatacc tcatctaacg ccctgttcgc
     7621 cgcgctgcaa gccaccgcag cacaacaaat tggcgtctct gatctgttgc tggttgccgc
     7681 caataccacc ggtggcgtca ccggtaagat gatctccccg caatctatcg ctatcgcctg
     7741 tgcggcggta ggcctggtgg gcaaagagtc tgatttgttc cgctttactg tcaaacacag
     7801 cctgatcttc acctgtatgg tgggcgtgat caccacgctt caggcttatg tcttaacgtg
     7861 gatgattcct taatgattgt tttacccaga cgcctgtcag acgaggttgc cgatcgtgtg
     7921 cgggcgctga ttgatgaaaa aaacctggaa gcgggcatga agttgcccgc tgagcgccaa
     7981 ctggcgatgc aactcggcgt atcacgtaat tcactacgcg aggcgctggc aaaactggtg
     8041 agcgaaggcg tgctgctcag tcgacgcggc ggcgggacgt ttattcgctg gcgtcatgac
     8101 acatggtcgg agcaaaacat cgtccagccg ctaaaaacgc tgatggccga tgatccggat
     8161 tacagtttcg atattctgga agcccgctac gccattgaag ccagcaccgc atggcatgcg
     8221 gcaatgcgcg ccacacctgg cgacaaagaa aagattcagc tttgctttga agcaacgcta
     8281 agtgaagacc cggatatcgc ctcacaagcg gacgttcgtt ttcatctggc gattgccgaa
     8341 gcctcacata acatcgtgct gctgcaaacc atgcgcggtt tcttcgatgt cctgcaatcc
     8401 tcagtgaagc atagccgtca gcggatgtat ctggtgccac cggttttttc acaactgacc
     8461 gaacaacatc aggctgtcat tgacgccatt tttgctggtg atgccgacgg ggcgcgtaaa
     8521 gcaatgatgg cgcaccttag ttttgttcac accaccatga aacgattcga tgaagatcag
     8581 gctcgccacg cacggattac ccgcctgccc ggtgagcata atgagcattc gagggagaaa
     8641 aacgcatgat tatttccgca gccagcgatt atcgcgccgc agcgcaacgc attctgccgc
     8701 cgttcctgtt ccactatatg gatggtggtg catattctga atacacgctg cgccgcaacg
     8761 tggaagattt gtcagaagtg gcactgcgcc agcgtattct gaaaaacatg tccgacttaa
     8821 gcctggaaac gacgctgttt aatgagaaat tgtcgatgcc ggtagcactg gcaccggtgg
     8881 gtttgtgtgg catgtatgcg cgtcgtggcg aagttcaggc agccaaagcg gcggacgcgc
     8941 atggcattcc gtttactctc tcgacggttt ccgtttgccc gattgaagaa gtcgcgccag
     9001 ccatcaagcg cccaatgtgg ttccagcttt atgtactgcg cgatcgcggc tttatgcgta
     9061 acgcgctgga gcgagcaaaa gcagcgggtt gttcgacgct ggttttcacc gtggatatgc
     9121 cgacaccggg cgcacgctac cgtgatgcgc attcaggtat gagcggccca aatgcggcaa
     9181 tgcgccgcta cttgcaggcg gtgacgcatc cgcaatgggc gtgggatgtg ggcctgaacg
     9241 gtcgtccaca tgatttaggt aatatctcag cttatctcgg caaaccgacc ggactggaag
     9301 attacatcgg ctggctgggg aataacttcg atccgtccat ctcatggaaa gaccttgagt
     9361 ggatccgcga tttctgggat ggcccgatgg tgatcaaagg gatcctcgat ccggaagatg
     9421 cgcgcgatgc agtacgtttt ggtgctgatg gaattgtggt ttctaaccac ggtggccgcc
     9481 agctggacgg tgtactctct tccgctcgtg cgttgcccgc tattgcagat gcggtgaaag
     9541 gtgatatcgc cattctggcg gatagcggaa ttcgtaacgg gcttgatgtc gtgcgtatga
     9601 ttgcgctcgg tgccgacacc gtactgctgg gtcgtgcttt cctgtatgcg ctggcaacag
     9661 cgggccaggc gggtgtagct aacctgctaa atctgatcga gaaagagatg aaagtggcga
     9721 tgacgctgac tggcgcgaaa tcgatcagcg aaattacgca agattcgctg gtgcaggggc
     9781 tgggtaaaga gtttcctgcg gcactggctc cgatggcgaa agggaatgcg gcatagtcgt
     9841 ttgcgccccc tccccctaac cctctccctc agggagctgt ctcttgatca gatctcctga
     9901 tcaagagaca gggagagggc cggggtgagg ggaaaaggcc gcactgctca ccaattttct
     9961 gctatcctgc ccccgcacta agggggcagt atgctaaaca tcgtacttta cgaaccagaa
    10021 attccgccaa atactggcaa catcatccgt ctttgcgcta ataccggctt tcgtctgcat
    10081 atcatcgaac cgatgggatt tgcctgggac gataagcgcc tgcgccgcgc ggggttggac
    10141 tatcacgagt ttaccgccgt tacgcgtcat catgactttc gcgcgttcct cgaagcagaa
    10201 aatccccagc gcctgtttgc cctcaccacg aaaggtacgc ctgctcacag cgccgtaaac
    10261 tatcaggatg gcgattatct gatgttcggc ccggaaacac gcggcctgcc agcgagcatt
    10321 cttgatgccc tgcccgctga acaaaaaatt cgcattccga tggtgccgga cagccgcagc
    10381 atgaacctgt ccaatgcggt gtcagttgtg gtgtatgaag cctggaggca gctgggatat
    10441 ccgggagcgt tgttgagaga ttagttactg tatgccggat agatgattac atcgcatccg
    10501 gcacgatcac agcacatcag atcccatccc catactcaaa tgtatggtta ataccgttga
    10561 aatgctggtc catatccatt gatggcttat cgctgtctgg tttaccgaca atacgagccg
    10621 gaacgccagc ggcggttgta tgcggcggca ccggttgcag caccacggaa cctgcgccaa
    10681 tcttcgcgcc gcgaccaact tcaatattgc cgaggatttt cgcgcccgcg ccaatcatca
    10741 caccttcacg aattttcggg tgacggtcac caccagattt acccgtaccg ccaagcgtca
    10801 cagattgcag aatcgatacg tcgttttcaa ttaccgccgt ttcacccaca acgatgcccg
    10861 tggcgtggtc gagcataatg ccacggccaa tttttgctgc cgggtgaata tcgacctgga
    10921 acgtcacaga aacctggttt tgcagaaaga ttgccaatgc gcgacgcccc tgattccaca
    10981 accagtgacc gatgcgatag gcctgcaagg catgaaaacc cttcaggtat aacaacgggg
    11041 ttgagtattt atcgactgcc gggtcgcggg tacgcaccgc ctgaatatca caggccgcag
    11101 aggcgatcat ttccgggtca gcggcgtagg cttcttccac cacttcacgg atagcaatag
    11161 caggcataat tggcgatgac agcttgttcg ccagcatgta gctcagtgca ctaccaaggt
    11221 tttcgtgctt gagtagcgtc gcgtggtaaa aactggccag cattggctca cagtccgcca
    11281 gcgttctggc ttcggcttta atattgttcc agacaatttc cagttcttca cacgacattg
    11341 cttactccac acgatgagat aatgaccggc ccgttctgcg cgggccgggt catagcggta
    11401 acaaaggttc cctggggtta gtggctgctg cgctcgtcct tgcgtgcacg acctaataac
    11461 gtcaatgctg cctcgcgcgc gttttttccg caatataata cttgataaat ttcctcggtt
    11521 attggcattt caacgccgaa gcgatgcgcc agttcgcgga cttctttcgt attgcggtag
    11581 ccttccacca cctgaccaat cttctcctgc gcgctttgta catccatgcc ctgaccgagc
    11641 atcatgccaa aacggcggtt acgcgactgg ttgtcggtac aggtaagcac cagatcgccc
    11701 agcccggcca tgcccataaa ggtggcagga tcggcaccca gcgccgcgcc aagacgcgac
    11761 atttcagcca gcccacgggt gatcagcgcc gtacgcgcat tcgcaccaaa accgataccg
    11821 tcggacatcc ccgcaccaat ggcaatgacg tttttcaccg cgccgccaag ctgcacgcca
    11881 atgaaatccg gattgctgta aacgcggaaa cttttgccgc agtgcagcag ctgctggaga
    11941 tcatcggcaa aggtctgatc ggtcgaggcc agcgaaatag ctgtcggtaa acctgccgcc
    12001 agttctttcg caaacgttgg gccagagata accgccagcg gaatttgatc gcctaacgcc
    12061 tcgcgggcca cgtcctgtaa cagacgcccg gtttccgctt ccagcccttt ggtcgcccac
    12121 accagacgcg catcaggacg catcggcggt ttaatctggc gcagcacttc accaaagaca
    12181 tggctgggta cgacgacgag aatattacgg ctggctgcca gcgcagtggc gagatcgctt
    12241 tcaagatgga gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg
    12301 cgttcaagcg ttgcgatatg ttcagggtca tggccccaga ggacaacctc gtggccattt
    12361 cttgccaggg taatggcaag agcggtgccg tacgagccgg caccgatcac agtcattgaa
    12421 gcattacgtt ggttcatcag gcatcctgat gttcttcagt accttcgcca gcctgctgct
    12481 gcaaatagtt catgaacagc gcatcgaagt taaccggcgc aaggttcagt tgcgggaatg
    12541 taccgcggga taccatgctg gtgatgcact cacgagcata cgggaacaga atgttcgggc
    12601 agtatgcgcc caggcaatgt gccatctggg tgccttcgat acccgcgatg gagaaaatac
    12661 cgccctgctg aacttcacac aggaacgcgg tttcttcgcc cagggaggcc gttacggtaa
    12721 cacgcagtac cacttcgtat acgtcatctg ccagttggga agatgccgta tccagatcaa
    12781 gtttaacttc tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag
    12841 agatatcctt ggtataaata cgttggatct ggaaagtcat ttcagtgttg ttttgttctg
    12901 acatgtgtag aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa tgccaacgtt
    12961 cacacgttat ttcagcaggg gatccagtcc accacgtgca tccaatgcat acaagtcatc
    13021 acagccgcca atgtgctgtg cgtcaataaa aatctgggga accgtggtgc gaccgctgcg
    13081 tttgatcatc tcttcacgct tggcggcatt gccatcgatc ggcaactcct gaaaactcac
    13141 gcccttgctg ctcagcagtg cttttgcacg atggcaatac gggcaggttt ctttggtata
    13201 gatttcaaca ttggccataa tttagctcct gtttttttac cctgaaattt catgttgcag
    13261 ggcggctgca aaatggagtg tccccagaag cttataacaa taagtcgctg ggggcctaat
    13321 tgcagctaac ggcctgcatc atgaaagacg acaggtaaat tatttgccgc gcaccagagg
    13381 caagttttcg ccagcccagc cagcaacgcc ctctttcagc acgaatactt gcgcaaaacc
    13441 agctttcgtc agcgcgtttg caggctcctg gcactgcatg ccagaaccgt ctaccacgat
    13501 aaccggtttg tctttgtgct tctcaagctc accaacattg ttggctttga tttcactcgg
    13561 caacagattg atagaacctg cgatatggcc tttacggaag tcatcacgct gacgtaaatc
    13621 cacaaccaca gcgtcttctt tgttgatcag acgcgtagct tcaccacgac taatcacctt
    13681 cactttcgag gtcaggctct taaacgtagt cacaagaacc gccaccagta acgcgatcca
    13741 ggcgatactc agtatgggat gacggccaac aaattgcata atttcttgca tggggggtaa
    13801 caactcccga cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc ggtgcggcaa
    13861 atacagccag cgcgttaact ggaatgcaat tttgcggggc acgacgaaaa aaaagcgcaa
    13921 atctggcatc ggcttgccgc gagcggtcgt atattttgat cttcagaggc tattttatcg
    13981 attcagctgt agtaaaatta cgcaaatttt gactcttgag tatgaggttg tcgcaatgtc
    14041 ggtttctaaa aaacctatgg tactggtgat tctggatggc tatggctatc gtgaagaaca
    14101 gcaggataac gccattttta gtgctaaaac cccggtaatg gatgcactgt gggccaatcg
    14161 tccgcatacc ctgatcgacg cttccggtct ggaagtcggt ctgcctgacc gtcagatggg
    14221 taactccgaa gtaggccacg ttaacctggg tgccggccgc atcgtgtatc aggacctgac
    14281 tcgtctggac gttgaaatca aagatcgcgc tttctttgct aatccagtgc tgactggcgc
    14341 ggtagataaa gcgaaaaacg caggtaaagc agtacacatt atgggtctgc tctccgcagg
    14401 cggcgtacac agccacgaag atcacatcat ggcgatggta gaactggcag ctgaacgcgg
    14461 cgcagaaaaa atctacctgc acgcattcct tgacggtcgc gacaccccgc cgcgcagtgc
    14521 tgaatcctcg ctgaaaaaat ttgaagagaa atttgctgcg ctgggtaaag gccgcgtagc
    14581 gtccatcatt ggtcgttact acgcgatgga ccgcgataac cgttgggatc gcgttgaaaa
    14641 agcctatgac ctgctgactc tggcgcaggg tgagttccag gccgatactg ccgttgctgg
    14701 tttgcaggca gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga ccgttattcg
    14761 tgcagaaggt cagccagatg cggcaatgga agacggcgat gcgctgattt tcatgaactt
    14821 ccgtgctgac cgcgcgcgtg aaatcactcg tgcattcgtg aacgctgatt tcgatggctt
    14881 cgcgcgtaag aaagtggtta acgtcgattt cgtgatgctg accgaatacg ccgctgacat
    14941 caaaactgcg gttgcttacc cacccgcttc cctggttaac accttcggcg agtggatggc
    15001 gaaaaacgac aaaactcagt tgcgtatttc cgaaaccgaa aaatatgccc acgttacttt
    15061 cttcttcaac ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc tgatcaactc
    15121 accgaaagtg gctacctacg atctgcaacc ggaaatgagc tccgcagagc tgaccgaaaa
    15181 actggttgcg gcaatcaaga gcggcaaata cgacaccatt atctgtaact atccgaacgg
    15241 cgacatggta ggtcacaccg gggtgatgga agcagcggtt aaagcggttg aagcgctgga
    15301 tcactgcgtg gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac tgctgatcac
    15361 cgctgaccac ggtaacgctg aacagatgcg cgatccggca acgggtcagg cacacacggc
    15421 acacaccaac ctgccagttc cgctgattta cgttggcgat aagaacgtga aagcggttga
    15481 aggcggcaaa ctttctgaca tcgcgccgac catgttgtcg ctgatgggta tggaaatccc
    15541 gcaagagatg actggtaagc cgctgttcat cgtggaataa tccctcccca tgaggggaaa
    15601 ggcgattaat accatgacac gggccgtgaa accgcgcagg tttgcaatca ggcccatcat
    15661 ctacgccagc gttcttagcg ctggcgtatt gttgtgcgcc ttttccgccc acgcggatga
    15721 gcgtgaccaa ctcaaatcta ttcaggccga tatcgccgcg aaagagcgcg cggtacgcca
    15781 aaagcaacaa caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag aagcgatctc
    15841 tgaagccacc cgtaagctgc gcgaaacgca aaacacgctc aatcaactca ataaacagat
    15901 tgatgagatg aacgcgtcga ttgccaaact ggagcagcaa aaagccgccc aggagcgcag
    15961 cctcgccgca caactggatg ccgcattccg tcagggcgag cataccggta ttcagctgat
    16021 tctcagcggt gaagaaagcc agcgtggaca gcgtttacag gcttatttcg gctatctcaa
    16081 ccaggcgcga caagaaacca ttgcccagtt gaagcaaacg cgtgaagaag tcgccatgca
    16141 gcgtgctgaa ctggaagaga aacagagcga gcaacaaacg ctgttatatg agcagcgcgc
    16201 ccaacaggcg aaactgactc aggcgctgaa cgagcgtaaa aagacgctgg cggggctgga
    16261 atcttccatc cagcaaggtc agcagcagct aagtgaactg cgtgccaacg aatcccgtct
    16321 gcgtaacagc attgcccgtg cggaagccgc ggcgaaagcg cgtgcagaac gagaagcacg
    16381 tgaggcccag gcggttcgtg accgccagaa agaagcgacg cgcaaaggca ccacctataa
    16441 accgaccgaa agcgaaaaat cgctgatgtc ccgtaccggt ggtctgggtg cgccgcgtgg
    16501 tcaggcattc tggccggttc gcgggccgac gctgcatcgc tatggcgagc agctacaggg
    16561 tgaattacgc tggaaaggga tggtgattgg tgcctctgaa ggtactgaag ttaaagcgat
    16621 tgccgacggt cgggtgattc tggctgactg gctgcaaggt tacggcttgg tggtggtggt
    16681 tgagcatggt aaaggcgaca tgagtcttta cggctataat cagagcgcgc tggtgagcgt
    16741 tggttcgcag gttcgcgcgg gccagccgat tgcactggtg ggcagcagtg gcggtcaggg
    16801 tcggccttca ctctatttcg aaattcgccg ccagggtcag gcggtcaatc cacagccgtg
    16861 gttgggaaga taagttttgt ttccatttcg tcgtaacgtt cttgcatttg ccgctctgtt
    16921 ggcgctctcc tcccccgtac ttgctggcaa acttgccatc gtcattgatg attttgggta
    16981 tcgcccgcac aacgaaaacc aggtgctggc gatgccctcc gctatctccg tcgctgtatt
    17041 acccgattca ccgcacgcca gagagatggc gaccaaagcg cataacagcg ggcacgaagt
    17101 gttgattcat ctcccgatgg caccactgag taaacagccg ctggagaaaa atacgctgcg
    17161 cccggagatg agcagcgacg aaattgagcg cattattcgt agtgcggtca ataacgtgcc
    17221 ctatgccgtg gggatcaaca accacatggg tagcaagatg acctctaacc tgtttggtat
    17281 gcagaaagtg atgcaggcac tggagcgtta caatctttac ttcctcgaca gcgtaaccat
    17341 cggtaatacc caggcgatgc gcgccgcgca aggtactggc gtgaaggtga tcaaacggaa
    17401 ggtgttcctc gacgattcgc agaacgaagc ggacatccgt gtgcaattta atcgcgcaat
    17461 tgatctggcg cgtcgcaacg gttcaaccat tgccattggg catcctcacc cttcaacggt
    17521 acgcgtgcta caacagatgg tttataacct gccgccagac attacgctgg tgaaagccag
    17581 cagcttgctg aatgaaccgc aggttgatac ttctacaccg ccgaaaaacg ctgtgcctga
    17641 tgcaccgcgt aatccgttcc gtggcgtgaa gctgtgcaaa ccgaagaaac cgatagaacc
    17701 tgtttatgct aatcgcttct ttgaagtatt aagcgaaagc atcagccaga gcacgctgat
    17761 cgtttacttc cagcatcagt ggcaaggctg gggcaaacag cccgaagcgg cgaagtttaa
    17821 cgctagcgca aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt tttgtcgcgc
    17881 cattgccata agcggaaaga ccacagtaac gcctggtaac cgaccttcac gctgcgcaca
    17941 ttggtaatca ggcgcttata cataccggaa gtaaatatct cggcaatcat ccgttggcgg
    18001 gtaagaatat ccggctcttt gcgcaccgca tggcaaacac gcaatgcttc gtaagttatt
    18061 tgctgatgaa attccggata aatcgtaatt ttgtcggcat aatttcgatt taatttctcc
    18121 agcaggcggg taatcttaat atagtgacgt tgataattaa ggtttttatt cccttgtcta
    18181 tgcaaccgac tcactgacgt attatgcaga taatatttat ataatgattg ctcggtatat
    18241 cgcgcacgca gcgcgttaaa catgaattct gttgtccaga caatatcctg atgatgtaat
    18301 ccggcaataa atttaatgtt atttttaaca ataacatcac ggcgataaac ccccatccag
    18361 acaacgtgag tccaacggcg cgaagaaagc cccatccgca gccagtccgg gccggttaat
    18421 acgccggttg agcgaaggcg atcgctgggg atggattgcc aggtttctcc cgtttcacga
    18481 aaacaccagt cagcgttgca ctgcgccacg tcgaggtcat cctctaacgc catggtcatc
    18541 agcgtttcgt acatggtggg atagacctca tcgtcagcat cgacaaaagc gacatatttg
    18601 cccgtcgcca cctcgatccc acgattacgc gctaccgatg cgccagcatt cgcctgatgc
    18661 aacaaacgaa cgtgcggata gttttctgcg taatgctttg ctatttcaac agaattatcc
    18721 gttgaaccat cgttaataat aatgatttcc agagcagtcc aggtttgcgt aattaaagat
    18781 tccatacaag tgcggaaatc atcgcccgca ttatataacg gaataataac actaagttta
    18841 ttggtgctgt tcatcataaa ttaccgacga cctctgaagt tatcctgacg ttttacaggc
    18901 tttgtattaa gaaactatta agcctgtgag atattatttt tacaggtgag agagtcactt
    18961 cagggtagag agataatgag agcagctata taagagatca gcgcaattac gatatcttta
    19021 ttacactgac aggattttac atatttaacg gattatcctc aggcaaaagg gctggaatac
    19081 cagcccttgt tcgtgttaat cccaactcag aattactttc ccggactggc ccgaacgcat
    19141 agcgtcaaag cccttctgga aatcatcgat agagaaacga tgggtaatga tcggcgagag
    19201 atccagacct gactgaatca gcgccgccat cttgtaccag gtttcaaaca tctcacgacc
    19261 gtaaatacct ttaatgaaca agcctttaaa gattactttg gtccagtcga tagacatatc
    19321 ggacggtgga atacccagca tcgcaatacg accgccgtgg ttcatggtat cgagcatggt
    19381 acgaaacgct ggcggcgcgc cggacatttc cagaccgaca tcaaagcctt cggtcatgcc
    19441 cagttcagcc atcacatcat taaggttttc tttcgcgacg ttaaccgcac gggtgatacc
    19501 cattttgcgc gccagctcaa ggcggtattc gttaacatcg gtgatcacca cattgcgtgc
    19561 accaacgtgt ttcgccaccg ccgcagccat aatacctatc ggacctgcac cagaaaccag
    19621 cacatcttcg cccaccagat cgaacgacag cgcggtatgc acggcgttac cgaagggatc
    19681 aaaaatggaa gccaggtcgt cggagatatt gtctgggatt ttgaaggcgt tgaacgccgg
    19741 gatcaccaga tattcggcga agcagcccgg acggttaacg ccgacgccga tcgtgttgcg
    19801 gcacagatgg gtacgaccgc cacgacagtt gcgacagtga ccgcaggtga tatggccttc
    19861 gccagaaacg cgatcgccga ttttgaagcc tttcacttcc tgaccaatac ctaccacttc
    19921 accgacatat tcatggccta cgaccatcgg caccgggatg gttttttgcg accactcatc
    19981 ccagttatag atgtgaacgt cagtcccgca gatggctgtt ttacggattt taatcagcag
    20041 atcgttatgc ccgagttccg gtacaggaac gtcggtcatc cagatgccct cttccgcttt
    20101 cagtttggat aacgctttca tctcacatcc tcaggcgata acgcccagtt gtttaccaat
    20161 acgcgtgaat gcttctactg cacgcgtaat ttgctcaggg gtatgcgccg cagacatctg
    20221 ggtacgaata cgcgcctgac ctttcggaac gaccggatag aagaaaccgg tgacgtaaat
    20281 gccctctttt tgcagctcac gggcaaattt ctgcgccact actgcatcgc caagcatgac
    20341 cggaataatg gcatgatcgg ctcccgccag ggtaaagccc gccgccgaca tttgctcacg
    20401 gaactggcgc gcgttcgccc acagacggtc acgcagttcg ctgcccgctt cgaccatctc
    20461 cagtactttg atggacgcgg caacaatggc cggtgccagc gagttggaga acaggtacgg
    20521 acgagaacgc tggcgcagcc actcaaccac ctctttgcgc gccgcggtat aaccaccaga
    20581 agccccgccc agcgctttac caagtgtgcc ggtgataata tcgacccggc ccatcacatc
    20641 gcagtattca tgggaaccac gaccgttttc accgacaaaa ccgaccgcgt gggagtcgtc
    20701 taccatcacc agggcatcgt atttatctgc cagatcgcaa acgcccttca ggttggcaat
    20761 cacgccgtcc atcgagaaca caccatcggt ggcaatcagc acatgacgcg caccggcttc
    20821 acgcgcttct ttcagacgag cttcaagctc ctgcatatcg ttgttggcat agcgatagcg
    20881 tttagcttta cacagacgca caccatcaat aatagacgcg tggttcagtg cgtcggagat
    20941 aatggcgtct tccgcgccca gcagcgtttc aaacaggcca ccgttagcat caaagcagga
    21001 agagtagaga atcgcatctt ccatccccag gaatgccgcc agtttttgtt caagctcttt
    21061 atggctgtcc tgagtgccgc aaataaaacg caccgaagcc atgccgaaac cgtgagaatc
    21121 cattcccgcc tttgccgccg caatcagata cggatgattc gccagcccga gatagttatt
    21181 ggcacaaaag ttaatgacgt ggcttccatc agccacagtg atatctgctt gctgcgcaga
    21241 cgtaataatg cgctcttctt taaacaaccc ttccgcccgt gcggtttcca gatcgttggt
    21301 taactgctga taaaattctc cacgcattgc gattctccag acagggcaaa ttccagcaca
    21361 tattacccaa acttataggt cgcgacgaga tgacgcgtta acacttctgc aaaattcagg
    21421 ataaataacg tgtaacttga tgtctctcaa cacctacaga tagtagaaaa actgtacagt
    21481 tttggctggg cataatgatg attgatttta ctgcattgat ataaaaagaa aaaatgatgc
    21541 caaagcatca tttttcctat ctcagttggt atcaaaacgc tacccactaa tggagggaaa
    21601 cctattttct tgaaaggaaa attctcagcg cctgcatttt tgcccctaaa gtgttactcc
    21661 tgaagcagcg aaacaaatcg aaccattttc ctctccccag atagtttagg ttgaagagat
    21721 cggggcgttt gtaatagcac atgacaaaaa tgccctggtc atcatccaca atgttattat
    21781 ttagtgttat tttttgactt tccagaacta atttatagaa ttctttccat ttatgctgtg
    21841 aaccaacaat tgcgccccca ataatatata catgattgcc aatcatgaaa tcaaaaacct
    21901 gttgttgaga tgtgacagta agcccttttt ttatagtgaa aagatgcatc ttactttcat
    21961 caaatgggaa atcccaaatt ttcaatcctc gagttacatt tggcttacgg caatagccaa
    22021 aatctatcca cgcaacaaga ggcgttttca ctaagcccat gttgatagct ttatttacaa
    22081 aataggcctt aaggttacat accaaaacat actctggtga ccagtactct gggtttttta
    22141 attgtcgagg ctcaagtcta tttgtaaacg actcatcttt ttgaattttt tcgatacgac
    22201 ttctgatata tctaaatttc ttttttatat cgataacgat aaccgttgtt ggttttccgt
    22261 tgcgaatcgc ttcaaccctt ggtttcaaat cgggagaagt gaaaataatc atctcatttt
    22321 caagtgcggc cagacgctca aagtaactaa aataaacatc aactgaacga gctagttttt
    22381 cacggaaccc tttattagca gtccaatccc ctctaccaat gtcaaaatat gcagtgataa
//
LOCUS       DAZGAN020000073        22170 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGAN020000073 DAZGAN020000000
VERSION     DAZGAN020000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.073"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1006)
                     /gene="malZ"
                     /locus_tag="ACHKYO_RS15590"
                     /old_locus_tag="pgaptmp_003118"
     CDS             complement(<1..1006)
                     /gene="malZ"
                     /locus_tag="ACHKYO_RS15590"
                     /old_locus_tag="pgaptmp_003118"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HGU4429930.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     R"
     gene            complement(1162..2535)
                     /gene="proY"
                     /locus_tag="ACHKYO_RS15595"
                     /old_locus_tag="pgaptmp_003119"
     CDS             complement(1162..2535)
                     /gene="proY"
                     /locus_tag="ACHKYO_RS15595"
                     /old_locus_tag="pgaptmp_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HGU4429931.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(2611..3930)
                     /gene="brnQ"
                     /locus_tag="ACHKYO_RS15600"
                     /old_locus_tag="pgaptmp_003120"
     CDS             complement(2611..3930)
                     /gene="brnQ"
                     /locus_tag="ACHKYO_RS15600"
                     /old_locus_tag="pgaptmp_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HGU4429932.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(4337..5632)
                     /gene="phoR"
                     /locus_tag="ACHKYO_RS15605"
                     /old_locus_tag="pgaptmp_003121"
     CDS             complement(4337..5632)
                     /gene="phoR"
                     /locus_tag="ACHKYO_RS15605"
                     /old_locus_tag="pgaptmp_003121"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HGU4429933.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(5690..6379)
                     /gene="phoB"
                     /locus_tag="ACHKYO_RS15610"
                     /old_locus_tag="pgaptmp_003122"
     CDS             complement(5690..6379)
                     /gene="phoB"
                     /locus_tag="ACHKYO_RS15610"
                     /old_locus_tag="pgaptmp_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HGU4429934.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            6569..7771
                     /gene="sbcD"
                     /locus_tag="ACHKYO_RS15615"
                     /old_locus_tag="pgaptmp_003123"
     CDS             6569..7771
                     /gene="sbcD"
                     /locus_tag="ACHKYO_RS15615"
                     /old_locus_tag="pgaptmp_003123"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HGU4429935.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQQYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKTQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            7768..10911
                     /gene="sbcC"
                     /locus_tag="ACHKYO_RS15620"
                     /old_locus_tag="pgaptmp_003124"
     CDS             7768..10911
                     /gene="sbcC"
                     /locus_tag="ACHKYO_RS15620"
                     /old_locus_tag="pgaptmp_003124"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HGU4429936.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     ANQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            11037..12221
                     /gene="araJ"
                     /locus_tag="ACHKYO_RS15625"
                     /old_locus_tag="pgaptmp_003125"
     CDS             11037..12221
                     /gene="araJ"
                     /locus_tag="ACHKYO_RS15625"
                     /old_locus_tag="pgaptmp_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HGU4429937.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLHSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(12364..13272)
                     /gene="mak"
                     /locus_tag="ACHKYO_RS15630"
                     /old_locus_tag="pgaptmp_003126"
     CDS             complement(12364..13272)
                     /gene="mak"
                     /locus_tag="ACHKYO_RS15630"
                     /old_locus_tag="pgaptmp_003126"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HGU4429938.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            13397..14308
                     /gene="rdgC"
                     /locus_tag="ACHKYO_RS15635"
                     /old_locus_tag="pgaptmp_003127"
     CDS             13397..14308
                     /gene="rdgC"
                     /locus_tag="ACHKYO_RS15635"
                     /old_locus_tag="pgaptmp_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HGU4429939.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(14386..14469)
                     /locus_tag="ACHKYO_RS15640"
                     /old_locus_tag="pgaptmp_003128"
     CDS             complement(14386..14469)
                     /locus_tag="ACHKYO_RS15640"
                     /old_locus_tag="pgaptmp_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429940.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(14466..14807)
                     /locus_tag="ACHKYO_RS15645"
                     /old_locus_tag="pgaptmp_003129"
                     /pseudo
     CDS             complement(14466..14807)
                     /locus_tag="ACHKYO_RS15645"
                     /old_locus_tag="pgaptmp_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(14955..15239)
                     /gene="ppnP"
                     /locus_tag="ACHKYO_RS15650"
                     /old_locus_tag="pgaptmp_003130"
     CDS             complement(14955..15239)
                     /gene="ppnP"
                     /locus_tag="ACHKYO_RS15650"
                     /old_locus_tag="pgaptmp_003130"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HGU4429941.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(15311..15988)
                     /gene="aroM"
                     /locus_tag="ACHKYO_RS15655"
                     /old_locus_tag="pgaptmp_003131"
     CDS             complement(15311..15988)
                     /gene="aroM"
                     /locus_tag="ACHKYO_RS15655"
                     /old_locus_tag="pgaptmp_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HGU4429942.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(16246..16437)
                     /gene="yaiA"
                     /locus_tag="ACHKYO_RS15660"
                     /old_locus_tag="pgaptmp_003132"
     CDS             complement(16246..16437)
                     /gene="yaiA"
                     /locus_tag="ACHKYO_RS15660"
                     /old_locus_tag="pgaptmp_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HGU4429943.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(16487..17011)
                     /gene="aroL"
                     /locus_tag="ACHKYO_RS15665"
                     /old_locus_tag="pgaptmp_003133"
     CDS             complement(16487..17011)
                     /gene="aroL"
                     /locus_tag="ACHKYO_RS15665"
                     /old_locus_tag="pgaptmp_003133"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HGU4429944.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQSAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(17194..17652)
                     /gene="yaiI"
                     /locus_tag="ACHKYO_RS15670"
                     /old_locus_tag="pgaptmp_003134"
     CDS             complement(17194..17652)
                     /gene="yaiI"
                     /locus_tag="ACHKYO_RS15670"
                     /old_locus_tag="pgaptmp_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HGU4429945.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            17772..18581
                     /gene="proC"
                     /locus_tag="ACHKYO_RS15675"
                     /old_locus_tag="pgaptmp_003135"
     CDS             17772..18581
                     /gene="proC"
                     /locus_tag="ACHKYO_RS15675"
                     /old_locus_tag="pgaptmp_003135"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGU4429946.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(18598..19713)
                     /gene="adrA"
                     /locus_tag="ACHKYO_RS15680"
                     /old_locus_tag="pgaptmp_003136"
                     /gene_synonym="dgcC"
     CDS             complement(18598..19713)
                     /gene="adrA"
                     /locus_tag="ACHKYO_RS15680"
                     /old_locus_tag="pgaptmp_003136"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HGU4429947.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(19815..20135)
                     /gene="psiF"
                     /locus_tag="ACHKYO_RS15685"
                     /old_locus_tag="pgaptmp_003137"
     CDS             complement(19815..20135)
                     /gene="psiF"
                     /locus_tag="ACHKYO_RS15685"
                     /old_locus_tag="pgaptmp_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HGU4429948.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(20254..21669)
                     /gene="phoA"
                     /locus_tag="ACHKYO_RS15690"
                     /old_locus_tag="pgaptmp_003138"
     CDS             complement(20254..21669)
                     /gene="phoA"
                     /locus_tag="ACHKYO_RS15690"
                     /old_locus_tag="pgaptmp_003138"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HGU4429949.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(21770..22030)
                     /gene="iraP"
                     /locus_tag="ACHKYO_RS15695"
                     /old_locus_tag="pgaptmp_003139"
     CDS             complement(21770..22030)
                     /gene="iraP"
                     /locus_tag="ACHKYO_RS15695"
                     /old_locus_tag="pgaptmp_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HGU4429950.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         5570 a         5943 c         5527 g         5130 t
ORIGIN      
        1 gccgccagcc gtccatactc cacggcgctt tcagccaatg gcggacaata ctgtcttccc
       61 cgcgataaat ttcattcacc agactttccg actgataatc cagcttcggc aagctggcat
      121 agccaagcca gtcgagcgcc gtgccatcat cactaaacga gtaccagtcg cgccagggcg
      181 attcagggtt gtgacaagct ccccccgtgc cacgattgtg cctgtcaaac caggcatggg
      241 aatcgccact gtggttaaac acgccatcca gcaccagccg cattcccagc tgctgcgtat
      301 tgtggcgtaa gcgcagcaac gccccatcac cgccaaactg cggatcgaca tggcgataat
      361 cctcggtatc gtatttatgt acgctgggag ctttaaacac cggattgaga tacagcgccg
      421 tcacgccaag ctttttcaga tacggcagtt tttcgcttat cccgtccaga tcgccgccat
      481 agaacgttga tccgcccgcc tgcgccgtga ccggttcatc ccagtcacgc aagatgatct
      541 cttgtccggc tgcatgatgg taatagacat gatcctgttc agcttcacga ggaagactac
      601 gcgcaaaacg atcagggaag atctgataaa aaatctgatc cgcagcccat tgtgggccga
      661 tatccggtac atcgacggca aactgctcca gtcgtgccgg cggcattcgg ctgaagccct
      721 gcggtgtaaa ccaacgctgg cgatcgtgcc acagcagttt gaaactgtaa cgccgccggg
      781 gttgtccgct ggagagatca atcgccgcac gccatgcggt gacgccaggc tgctgctgac
      841 tgcgctgctt atgcatcgat actgacattt cttcgttatc gtgttctgta cgcagcataa
      901 tgcgctgcgg tgggtcttcg cccgtcagcc acagtgtaat gagcagttga tctttgcttt
      961 gtttaacaaa tgggggcacc ggcaggtgcc atgcatttaa catcataatt cccctgtgat
     1021 gaaaatctga tcaccttgcc atagcctaat gcaggatcac tcatcatctg aaggatattt
     1081 gggggaggag gacgggggcg gataactttc ggaatagccg gatcaggatt tcctcctgaa
     1141 ccggcttaca gaacgcaagg attactggtt ttcagccagc tgacgatcgt ggcggcgttt
     1201 aaacatccag ccaatcaaca gcacaacaat ccacgcgaaa ccgacataca gcgagatacg
     1261 cgtatccggg tgataaccaa tcaacccgat aataaagagc aggaaaatca aaccgccgat
     1321 ggtcgttgct accccacccg gcactttaaa tttcagcgcc ttaacttctt ctggcggcaa
     1381 acggcgacgg aaggcaattt gcgacagcag gatcataatc cacacccaca ccgtggcgaa
     1441 ggttgccagc gaagcgatca ccaggaagac gttttccggc atgatgtagt tcagatacac
     1501 cgcaaacagc agcgcggtag tcatcaccag caccgtaacc cacggaatac cgcgacgcga
     1561 cgttttgctg aaaattttcg gcgcgctgcc ctgctctgcc ataccgtgga gcatacggcc
     1621 tacgccaaat acgtcactgt taattgccga cagcgaagca gtcagcacaa caaagttaag
     1681 aatgctggcg gcaaaggtaa tgcccatatg ctggaacgtc agcacgaacg ggctaccggc
     1741 agtgccaacc tgattccacg ggtagataga cataatgacg aacagcgtac cgacgtagaa
     1801 caccagaata cgcatcggca cggagttaat cgcacgcggt atcgatttct caggatcttt
     1861 cgcttcaccg gcggtaatcc cgataatttc gatcccaccg taagcaaaca tcaccatttg
     1921 caacgacatc accatcccaa gccagccgtt actgaagaag ccgccgttgc tccacaggtt
     1981 atgaataccg gtcggttgcc cgccgttgcc aatcccccag atgatgatgc cgaaaccggc
     2041 gacaatcatg atgatgatgg tggcgacttt aaagaacgag aaccagaatt ccagctcacc
     2101 gaagaccttc acgctcatca ggtttacggc gcaaatgatc agcaccacgc tcagtaccca
     2161 aatccagtgc ggcaccgtcg ggaaccagac acccatatag ataccaaaag cggtcacatc
     2221 ggcgatggcg acaataagga tttcaaagca ataggtccag ccggtaatgt aacctgccag
     2281 cgggccgagg ttttcctgcg cataacgcga gaaagagcta gctgccgggt tatgtaccga
     2341 catttccccc agcgcacgca taatgatata cgccgcgata ccaccgataa tataggccaa
     2401 caacacgctc ggaccggcca ttttgatggc gtctgccgaa ccgtaaaaca gcccggtgcc
     2461 aattgctgaa cccagtgcca taaagcgtat gtggcgggta cttagcccac gctttagctt
     2521 gttcttactt tccatcaatt cctaacccat caacacaata aaaaaccacg gggcatcgca
     2581 gccccgtggt taaaacaaat gttcagtgat ttagtgagcg ctggaggtca cctgacgacc
     2641 tgccgcgcga tcccagataa tggccagaac caccatcacc actgttggca ttaaccacgc
     2701 cagaccttgt tcggccagcg gtaaacgctg cgcccaggac ggtaagatat cgctgaatgc
     2761 agatgctttg atcccgtcga gaataccaaa aagcaggctg ataaacatcg gcggagcaat
     2821 cacgcgggac gaattatgcc accatgagcg tgtaaaactt aatacaacca gtgcgataca
     2881 cggcggataa atagcggtca gcaccggtac ggagatctgg atcagctggc ttaagccgag
     2941 gttagaaacc accatcgaga agccgccgag gataaacacc agcgtacgat aagagagcgg
     3001 tacgtactgg gcaaagaatt ctgcacaagc acaggtcagg ccaactgccg ttaccaggca
     3061 ggcgatgaag attaacgccg ccagcaggaa gctaccgccg ccgccaaagg tgtgctgaac
     3121 gtaagcatgc agaatagcag cgccgtttgc agactgatcg accagcgacg cgctgtctga
     3181 acccagacgg aacagcgcca ggtacagcag agtcagacca acacccgcca tcaggccagc
     3241 ccagacggta taacgggtca gcagacgcgc ttcggtaacg ccacgagaac gcgccgcgtt
     3301 aacaataacg ataccaaaca ccattgcgcc cagcgtatcc atggtcagat agccgttaac
     3361 gaagccgtta gaaaacgcag cgttttgata agcctcagtc gccgtgctga tagaacccgc
     3421 cggccagaca atcgcggcaa cagacaggat gaccagcgcg ataattttca gcggcgcaag
     3481 gaagttgccc acggtatcca gcagcttgcc cggatagagc gaaaccagaa taacgatagc
     3541 gaaatagacc aggctgtaga taaacagcgg cagcgcggaa tcaccagtca gcggcgcaat
     3601 ccccacttca aaggaaacgg tagctgtacg cggcgtagcg aaaagcggcc ccaccgccag
     3661 gtaacaaacc gttgccagca gtacgccagc gactttaccg attggcgtgc tgaggctgtc
     3721 aacaccgccg ccaacttttg ccagcgccac taccgttagt accggcagac caacggcagt
     3781 aatgaggaag ccgaatgccg cagtccagac gtgttcgcct gcctgcaagc cgaccattgg
     3841 agggaaaata atgttacctg cgccgacgaa caacgcaaat gtcataaagc ccagagcgat
     3901 gatatcgcgc gatcttaatt gatgggtcat aaatcatact gcctgtggat gtggtgttgt
     3961 aacgttaaat ttttgtgcca atccctgggg atgatacagc ggtatattta aacaatttta
     4021 gcgggaaata ctcccaacca ggcgcggcga agaatagttt ttcgatttac cgcgttaatg
     4081 cagtcagtat gacagcacct gaagcgcaat ttaaacgctt ataacgtttt aaagcaagga
     4141 gggatctaaa aaccagaggt atatagcgat aagaaatcac caaccagtat aaaacactat
     4201 ttccgtgaaa aagacatggc taatcatgcg aacaaaaaat caccaaccgt tgtaaaatct
     4261 aacgcccgta ttttgactgc aaagaaaata agccagcatc ggctggctta tggcaataaa
     4321 agatgacaaa ggcggattaa tcgctgtttt tggcaattaa acgttccggg ataacaaaac
     4381 tgaaacgtgt tccttttcct actgtactct caatattcag gcgactttcg tgatgattca
     4441 ccgcatgttt cacgatcgct aaccctaatc cgctaccgcc ggtttgccgg gaacgcgctt
     4501 tatcaacgcg ataaaaacgc tcggtcaggc gcggaatatg ctccggtgca atgcccggtc
     4561 cgttatcttc aacgctaaat tcggcaccgt gcggcactcg ctgccagcgt acggtgatat
     4621 gcgtgccttc tggagtatga ttcacggcgt tatagaccag gttcgaaatc gcactgcgta
     4681 gctgatcttc gttgccagac actttgaggc cgttatctat ctcaaaggta aatgtctgtt
     4741 ttttctgact cagagtctga gcctcacgct caacaacgcg cagcatcatc ggcacatcaa
     4801 ccttttcatt gagcaaatgc gtcggtgcgg cttctatttt cgacagcgtc agcaactgct
     4861 tcaccagccc ttccatccgc tgggtctgct cgcgcatggt gtgcaacgct ttttcgcgta
     4921 ccgcgccttc cagcggctgc tcatccatca tctccaggta accctgtaac acggtcaatg
     4981 gcgtgcgtaa ctcatggctc acgttggcaa aaaagttacg ccgcgcccct tccagttgat
     5041 gcatttgcgt gacatcacgc gccaccatca gcaactgttt gtgggtataa ggcatgacgc
     5101 gaatttccag atgccgcccg gtgttgagca ccagattgag cgggcgagaa aaatcacgcg
     5161 ttttcagata ttgcgtaaac tccgggtaac gcagtaggtt aaggatgttc tgcccgttat
     5221 cttccggcca gcgcaaacca agaatttgtt gcgccagacc gttacaccag aaaataccgc
     5281 cctcttccgt ggtcagcacc accgcgtcgg gcagcgactc cgcgccgcta cgaaagcgtt
     5341 taatcagatt gcccagttca cggcggcgtt ttttatttcg cagctgcatc tggtgtaagc
     5401 cgtatagtag cggttcccag ctaccacgcc ccggtggcgg ggtcatactg cgatccaccc
     5461 acagccacca tgaaaggcgc aataaattcc agaaatgcca gataagcagt cctgttaccg
     5521 atgccagcaa aaaccagggc aggtaaccaa aaaatgcacc caggatgaaa gccgggaggc
     5581 agcaaagtag cagctccagc accagccttt tccacgacag ccgttccagc acgtaagata
     5641 ctccagttaa gaaatcataa gccctgctct gcgtccgatg agcaaggcgt taaaagcggg
     5701 ttgaaaaacg atatcctgta ccgcgcacgg tctgcaccat gcggtcatgc ccgccgggct
     5761 ccagtgcttt acgcaggcga cgaatgtgga catcgaccgt gcggtcttcc acatacacgt
     5821 tagttcccca gacgtggttt aacagctgct cgcggctgta cacgcgctca ggatgcgtca
     5881 taaaaaagtg cagcagttta aattctgtcg gccccatctc cagcggctct tcgcccgcca
     5941 tcactcggtg agatgtcggg tcgagactta atccctgcat ctcaatcacc tcttccaccg
     6001 ccattggcga aatacggcgc attaccgctt tgattcgcgc caccagctcc ttcggcgaaa
     6061 acggcttggt gatatagtca tccgcgccgg tttcaaggcc gcgcacgcga tcttcttctt
     6121 cccctctggc ggtcaacatc accactggaa tatcccgggt catcgactcg cgcttgaggt
     6181 gtttgatgaa ctggataccg gagccgccag gtaacatcca gtcaaggaga attaaatccg
     6241 gccagggttc attcagttga ttcacagcac tgtcataatc ttccgcttcg accggctgaa
     6301 agccattttg ttcgagcacg aagcagacca tttcgcgaat tggagcttca tcttctacga
     6361 ccagaatacg tctcgccatg atttgccctg ttgtaataaa taggttgcga tcattaatgc
     6421 gacgtcatta tgcgtcagat ttatgacaga tttatgaaaa gctcgtcgca catatcttca
     6481 ggttattgat ttccgtggcg cagaaaaaag caaatggcac atctgtttgg gtataatcgc
     6541 gcccatgctt tttcgccagg gaatcgttat gcgcatcctt cacacctcag actggcatct
     6601 cggccagaac ttctacagta aaagccgcga agctgaacat caggcttttc ttgactggct
     6661 gctggaaaca gcacaaaccc atcaggtgga tgcgattatt gttgctggtg atgttttcga
     6721 taccggctcg ccgcccagtt acgcccgcac gttatacaac cgttttgttg tcaatttaca
     6781 gcaaactggc tgtcatctgg tggtactggc aggaaaccat gactcggtcg ccacgctgaa
     6841 tgaatcgcgc gatatcatgg cgttcctcaa tacgaccgtg gtcgccagcg ccggacatgc
     6901 gccgcaaatc ttgcctcgtc gcgacgggac gccaggcgca gtgctgtgcc ccattccgtt
     6961 tttacgtccg cgtgacatta ttaccagcca ggcggggctt aacggtattg aaaaacagca
     7021 gcatttactg gcagcgatta ccgattatta ccaacaacaa tatgccgatg cctgcaaact
     7081 gcgcggcgat cagcctctgc ccatcatcgc cacgggacac ttaacgaccg tgggtgccag
     7141 taaaagtgac gccgtgcgtg acatttatat tggcacgctg gacgcgtttc cagcacaaaa
     7201 ctttccacca gccgactaca tcgcgctcgg gcatattcac cgcgcacaga ttattggcgg
     7261 catggaacat gttcgctatt gcggctctcc cattccactg agttttgatg aatgcggaaa
     7321 gagtaaatat gtccatctgg tgacattttc aaacggcaaa ttagagagcg tggaaaacct
     7381 gaacgtaccg gtaacgcaac ccatggcggt gctgaaaggc gatctggcgt cgattaccgc
     7441 acagctggaa cagtggcgcg atgtatcaca ggagccgcct gtctggctgg atatcgaaat
     7501 cactactgat gagtatctgc atgatattca gcgcaaaacc caggcattaa ccgaatcatt
     7561 gcctgttgaa gtattgctgg tacgccggag tcgtgaacag cgcgagcgtg tgttagccag
     7621 ccaacagcgt gaaaccctca gcgaactcag cgtcgaagag gtgttcaatc gccgtctggc
     7681 actggaagaa ctggatgaat cgcagcagca acgtctgcag catcttttca ccacgacgtt
     7741 gcataccctc gccggagaac acgaagcatg aaaattctca gcctgcgcct gaaaaacctg
     7801 aactcattaa aaggcgaatg gaagattgat ttcacccgcg agccgttcgc cagcaacggg
     7861 ctgtttgcta ttaccggccc aacaggtgcg gggaaaacca ccctgctgga cgccatttgt
     7921 ctggcgctgt atcacgaaac tccgcgtctc tctaacgttt cacaatcgca aaatgatctc
     7981 atgacccgcg ataccgccga atgtctggcg gaggtggagt ttgaagtgaa aggtgaagcg
     8041 taccgtgcat tctggagcca gaatcgggcg cgtaaccaac ccgacggtaa tttgcaggtg
     8101 ccacgcgtag agctggcgcg ctgcgccgac ggcaaaattc tcgccgacaa agtgaaagat
     8161 aagctggaac tgacagcgac gttaaccggg ctggattacg ggcgcttcac ccgttcgatg
     8221 ctgctttcgc aggggcaatt tgctgccttc ctgaatgcca aacccaaaga acgcgcggaa
     8281 ttgctcgagg agttaaccgg cactgaaatc tacgggcaaa tctcggcgat ggtttttgag
     8341 cagcacaaat cggcccgcac agagctggag aagctgcaag cgcaggccag cggcgtcgcg
     8401 ttgctcacgc cggaacaagt gcaatcgctg acagcgagtt tgcaggtact tactgacgaa
     8461 gaaaaacagt tacttaccgc gcagcagcaa gaacaacaat cactaaactg gttaacgcgt
     8521 ctggacgaat tgcagcaaga agccagccgc cgtcagcagg ccttgcaaca ggcgttagcc
     8581 gaagaagaaa aagcgcaacc tcaactggcg gcgctcagtc tggcacaacc ggcacgaaat
     8641 cttcgtccgc actgggaacg catcgcagaa cacagcgcgg cgctggcgca tactcgccag
     8701 cagattgaag aagtaaatac tcgcttacag agcacaatgg cgcttcgcgc gagcattcgc
     8761 caccacgcgg cgaatcagtc agcagaatta cagcagcagc aacaaagcct gaatacctgg
     8821 ttacaggaac acgaccgctt ccgtcagtgg aacaacgaac tggcgggttg gcgtgcgcag
     8881 ttctcccaac aaaccagcga tcgcgagcat ctgcggcaat ggcagcaaca gttaacccat
     8941 gctgagcaaa aacttaatac gcttgcggcg atcacgttga cgttaaccgc cgatgaagtt
     9001 gctagcgccc tggcgcaaca tgccgagcaa cgcccactgc gtcagcgcct ggtcgcgctg
     9061 catgggcaga ttgttcccca acaaaaacgt ctggcgcagt tacaggtcgc tatccagaat
     9121 gtcacgcaag aacagacgca acgtaacgcc gcacttaacg aaatgcgcca gcgttataaa
     9181 gaaaagacgc agcaacttgc cgatgtgaaa accatttgcg agcaggaagc gcgcatcaaa
     9241 acgctggaag ctcaacgtgc acagttacag gcgggtcagc cttgtccact ttgtggttcc
     9301 accagccacc cggcggtcga ggcgtatcag gcgctggagc ctggcgttaa tcagtctcga
     9361 ttactggcgc tggaaaacga agttaaaaag ctcggcgaag aaggtgcggc gctgcgcggg
     9421 caactggatg cattaacgaa gcagcttcag cgcgatgaaa acgaagcgca aagcctccga
     9481 caagatgagc aagcacttac tcaacaatgg caagccgtca cggccagcct caatatcacc
     9541 ttgcagccac aggacgatat tcaaccgtgg cttgatgcac aagatgagca cgaacgccag
     9601 ctgcggttac tcagccaacg acatgaatta caagggcaga ttgccgcgca taatcagcaa
     9661 attatccagt atcaacagca aattgaacaa cgccagcagc agctcttaac ggcattggcg
     9721 ggttatgcac tgacattgcc acaggaagat gaagaagaga gctggttggc gacacgtcag
     9781 caagaagcgc agagctggca gcaacgccag aacgaattaa ccgcgctgca aaaccgtatt
     9841 cagcagctga cgccgattct ggaaacgttg ccgcaaagtg atgatctccc gcacagcgaa
     9901 gaaactgtgg cgctggataa ctggcggcag gttcatgaac aatgtctcgc attacacagc
     9961 cagcagcaga cgttacagca acaggatgtt ctggcggcgc aaagtctgca aaaagcccag
    10021 gcgcagtttg acaccgcgct acaggccagc gtctttgacg atcagcaggc gttccttgcg
    10081 gcgctaatgg atgaacaaac actaacgcag ctggaacagc tcaagcagaa tctggaaaac
    10141 cagcgccgtc aggcgcaaac actggtcact cagacagcag aaacgctggc acagcatcaa
    10201 caacaccgac ctgacgggtt ggctctcact gtgacggtgg agcagattca gcaagagtta
    10261 gcgcaaactc accaaaagtt gcgtgaaaac accacgagtc aaggcgagat tcgccagcag
    10321 ctgaagcagg atgcagataa ccgtcagcaa caacaaacct taatgcagca aattgctcaa
    10381 atgacgcagc aggttgagga ctggggatat ctgaattcgc taataggttc caaagagggc
    10441 gataaattcc gcaagtttgc ccaggggctg acgctggata atttagtcca tctcgctaat
    10501 cagcaactta cccggctgca cgggcgctat ctgttacagc gcaaagccag cgaggcgctg
    10561 gaagtcgagg ttgttgatac ctggcaggca gatgcggtac gcgatacccg taccctttcc
    10621 ggcggcgaaa gtttcctcgt tagtctggcg ctggcgctgg cgctttcgga tctggtcagc
    10681 cataaaacac gtattgactc gctgttcctt gatgaaggtt ttggcacgct ggatagcgaa
    10741 acgctggata ccgcccttga tgcgctggat gccctgaacg ccagtggcaa aaccatcggt
    10801 gtgattagcc acgtagaagc gatgaaagag cgtattccgg tgcagatcaa agtgaaaaag
    10861 atcaacggcc tgggctacag caaactggaa agtacgtttg cagtgaaata actattcagc
    10921 aggataatga atacagaggg gcgaattatc tcttggcctt gctggtcgtt atcctgcaag
    10981 ctatcacttt attggctacg gtgattggta gccgttctgg tagttgtgat ggtggtatga
    11041 aaaaagtcat tttatctttg gctctgggca cgtttggttt ggggatggcc gaatttggca
    11101 ttatgggcgt gctcacggag ctggcgcata acgtaggaat ttcgattcct gctgccgggc
    11161 atatgatctc gtattatgcg ctgggagtgg tggtcggtgc gccaatcatc gcactctttt
    11221 ccagccgcta ctcactcaaa catatcttgt tgtttctggt ggcgttgtgc gtcattggca
    11281 acgccatgtt cacgctctct tcgtcttacc tgatgcttgc cattggtcgc ctggtttccg
    11341 gctttccgca tggcgcattt tttggcgtcg gcgcgatcgt gttatcaaaa attatcaaac
    11401 ccggaaaagt caccgccgcc gtggcgggga tggtttccgg gatgacagtc gccaatttgc
    11461 tgggcattcc gctgggaacg tatttaagtc aggaatttag ctggcgttac acctttttat
    11521 tgatcgctgt ttttaatatt gcggtgatgg catcggtcta tttttgggtg ccggatattc
    11581 gcgacgaggc gaaaggaaag ctgcgcgaac aatttcactt tttacacagc ccggccccgt
    11641 ggttaatttt cgccgccacg atgtttggca acgcaggtgt gtttgcctgg ttcagctacg
    11701 taaagccata catgatgttt atttccggtt tttcggaaac ggcgatgacc tttattatga
    11761 tgttagttgg gctagggatg gtgttgggaa atatgctaag tggcaggatt tcaggacgtt
    11821 attcaccact gcgcattgca gcagtgactg actttataat tgtactggca ctgctgatgc
    11881 tctttttctg cggcggcatg aaaacaacgt cgcttatttt tgcttttatt tgttgcgcgg
    11941 gattatttgc cctttcagca cctctgcaaa tattattact acaaaatgcc aaaggcggag
    12001 agttattagg tgccgcaggt gggcaaatag cgtttaacct tggtagcgcc gtcggcgcat
    12061 attgcggtgg tatgatgctg acgctggggc tggcatataa ttacgtggcg ctgcctgccg
    12121 ccctgctttc gtttgctgcg atgtcgtcat tgctgctgta tggtcgctat aagcgccagc
    12181 aagcggcgga tagtccggtg ctggcgaaac cactggggta gattgcagtc acgttgcgcg
    12241 ttgcttattg ccggatgcgg cgtgaacgcc ttatccgccc tacgcggttc cggcacattt
    12301 tgtaggcctg ataagacgcg gcaagcgtcg catcaagcat gatgcgccaa ttgcctacgt
    12361 tttttactct tgtggccata accacgcagc gccgcgtacg ccgctggaat caccgtgctt
    12421 cgccttacgc accggcgttt cacattcgcc gccgaagaca aattgtttaa tcaactgccc
    12481 aaccgtttga tataaacggt ctacattgct catcccgccc cccaggacaa tcacatccgg
    12541 atcgagaata ttcacgacat gtgccagcga ttttgccagc cgcagctcgt agcgacgcaa
    12601 tgccagttcc gctaccggat cgctttcttc aaccaggcgg ataatttcac tgcctttcag
    12661 cgcatgtccg ctcaaacgac gataatcagt cgcgaatccc gtgcccgaaa taaaggtttc
    12721 aatacaacct tgtttaccgc aataacaagg gacttcctcg cgataacgca gttcgtcttc
    12781 gtccatccac ggtagcggat tgtgtcccca ctcacctgcc gtgccattgc cgccgatatg
    12841 cgcccgccca ttgaatgcca cgcccgcgcc gcatcccgtg ccgataatca cggcaaatac
    12901 tgtctgcgct cccgctgccg cgccatctac tgcttctgaa accgccagac agttggcgtc
    12961 atttgccagc cgcacttccc gctgcaacct cgcgcttaag tctttatcga atggctgacc
    13021 gttgagccag gttgaattgg cattcttcac cacaccggtg taaggcgaaa ttgagccagg
    13081 aatgcccata cctaccgttc cgcgctgccc tgtcgcctgc tccgccatat caaccaacgt
    13141 ggcgatcgtt tcaatagttt gccggtaatc atcacgcggc gtgggcagac gatggcggta
    13201 caactgctcc cctgcatcgc ccagtgcaat cacttcagtt ttggtgccgc ctaaatcgat
    13261 acctatacgc acggtactct ccttattttt ttcaatatca atagcgtaga gacggacaac
    13321 cggattggca atgcaaggcc gcctacaatt cgttatcatg cccgctaaat ttaacgacaa
    13381 ggccgtggaa attatcatgc tgtggttcaa aaatttaatg gtttaccgtc ttagccgcga
    13441 gatttcgctg cgtgcagaag agatggaaaa acagctagcc tcgatggcat ttaccccatg
    13501 cggcagccag gacatggcga agatgggctg ggttcctccg atgggatcgc acagcgatgc
    13561 gttaacgcac gttgccaatg gtcaaattgt tatctgcgcg cgcaaagaag aaaaaatcct
    13621 cccgtctccg gtgattaaac aggcgcttga agcgaaaatc gccaaactgg aagcggaaca
    13681 ggcgcgtaag ctgaagaaaa ccgaaaaaga ttcgctgaaa gacgaagtgc tgcactctct
    13741 gctgccgcgt gctttcagcc gttttagcca gacaatgatg tggatcgaca cggttaacgg
    13801 tttgattatg gtggactgcg ccagtgccaa aaaagcggaa gatacgctgg cattactgcg
    13861 taaaagcctg gggtcgttac cggttgtacc attgagcatg gaaaacccga ttgaactgac
    13921 gctgaccgaa tgggttcgct ccggtagtgc ggcacagggc ttccagctgc ttgatgaagc
    13981 cgagctgaaa tcgttgctgg aagatggcgg cgtgatccgc gcgaagaaac aagatctgac
    14041 cagcgaagag atcaccaatc acattgaagc cggaaaagtg gtgactaaac tggcgctcga
    14101 ctggcagcag cgcattcagt ttgtgatgtg cgacgatggt tcgcttaagc gtctgaagtt
    14161 ctgcgacgag ctgcgcgatc aaaacgaaga tatcgaccgt gaagatttcg cccagcgttt
    14221 tgatgccgat ttcatcctga tgactggtga actggcagcg ttaattcaaa acctgattga
    14281 aggattaggt ggcgaagcac aacgttaatt gctgattttc ctttaatgcc ggatgcgacg
    14341 cctgccgcgt cttatccggc gtacgaagcc acaccaggca tataattatt cgctacggcg
    14401 ggcaataatt tttagcgcag caatattatg cgttttatgc tgtaacttgc tccatggacg
    14461 ttgtgtcatt gtttttcctc aagccagtga cgcgcttcac tgcgaatttt ccgcattgcc
    14521 ccggttttca ctaaatcccg taacagcgaa agatcgggtt gttcgacaaa taagagtaat
    14581 gatggatcct cttttaacca cgtcgttttc acatcggcag cgagctgtgg attattcata
    14641 gctagcgacc gatgccgaga ctcggtcaat gtcgttaaca acgacgccgg ggtatgtgcg
    14701 ttgcgtaatg ccgtttggcg caaatgatca ttgcgacttt gcagcaatgc acgccaccat
    14761 tcatcatctg atttatcttt tatcgtagcc agttccgcaa caatatctgg agatcttcac
    14821 tgatatttat atcaaccttg atgaattatg aatatatcgg aaattgaaaa aatcgcagca
    14881 aaggaaatat tattatgggt taaagagagg atatttcaga aaaatgccct cccgttcaag
    14941 gggaaggcga ggaattacag atagcggcac agataagagg tgggttcggc aacttgcaga
    15001 tgaaactcac tgtgaccggg aacattaaac accgaaccgg cttcatacac ctgccagtcg
    15061 gtcgcgtcag gcagtaacac attcagcgcg ccactgatta ccgtcatctc ttccggctca
    15121 gcggtgctga aggtgtattc gccttcaacc ataacaccca cgctggcgcg accagtgctg
    15181 ctgctggaaa agccgattga tttcactttg ccggaaaagt actcattact ttgaagcata
    15241 aactggccct gtctggtagt tgaacatcta cttcactata ggggccagag gcgctggctg
    15301 tcacgcaaaa ttacaccagt aattccgcag ccagccgtgc aatcaatacg ttagacagca
    15361 agacaggaac atcgagctgt ttttgcagta aatcgcgatg acgttggtga aatcctaaac
    15421 aatccagcat gatgacatca gccccttttg ccagtaattc tttcccggca tcaatgattt
    15481 tttgttctga atcatgaatg gggttgccca atgaaaatac cggcgatttc tgcaaaattt
    15541 gccatttttg cgcctgaacg gtcagcatct cctcaaccgg aacgataacc cccacctgat
    15601 gatcttcaac aatagaggaa accagtggag gcaatattcg cgacggctca agaaagatcg
    15661 tattacgcgc agtcatacta ctaatgtttg ctgtactcat taataaaatg acgtcataac
    15721 cctgattatc gagcacttca accacacctt gcaggtctcg ctccacttta cgacgcgaaa
    15781 catgggccag ctggttgtca tttaataatg tgagaatggt gtcttcgcct gcttctggcg
    15841 cgtactccgc catcacttct tcacgactta acttccccag caagctatga tgggaaatat
    15901 tatcttcgtc aatgtattcc gtcaggagcg gcaaaacctc ctgcataggt acaatgccga
    15961 tggtcaggat cgccaacgac gcactcattg ttcatccacc ttacttttct ttcgtcgtta
    16021 attaccgggc aagtgtgaag caccactgct gacacttact tgtgctacaa atgacaaaaa
    16081 gcgtagcagc agacgtgcac ggcataatgt aaagattcca aatgattcca gtaatggatt
    16141 gttattgttt aatattctaa ttattagaaa aacatgaatt atgaaaaatg tgacgcagat
    16201 cccttgtagt ggcactcaga atcccttccg agcagtctga tgcggtcact ctttgtcagg
    16261 gtcctcatag cgttttttcg catccatcgc ttcctgagcc gtcggatgtt cactgatcaa
    16321 cgagtctggt ttaggatgat cggctctgag ttggtaccag gttaccgtct gtcctggctt
    16381 tcctttttca atcgtcacta tatatgcttc acgaggataa ggcggtttcg ttggcatcgt
    16441 tcttccttat ttcacgggat gaacgttaag tataggcgct ctaaaatcaa caattgatcg
    16501 tctgtgccag ggcgctacga atttcagaaa tcacctggct gggttcgttt gttgcgtcga
    16561 tgataatatg cgcaacttcg cgatatagcg catcgcgttc ttccagcact tcctgaactt
    16621 cttcgctcag cggttttccc gttaaggttg gccgtaaatc ttcttccggt gcagattgca
    16681 gtcggttaac caggactgat actggcgcac acaaataaac cacgatcccg ttattttgca
    16741 tgaagtgacg attaaattcc gtcagaataa tgccgccgcc tgtagcgata acggtggatg
    16801 gcgcagttac cgcttccagc gccgccgttt ctctggcgcg aaatcccgcc cactcttccc
    16861 tttcgacgat ctccgcgacc gtcatattga gctgtgattg caaccactga tcggtatcga
    16921 caaaccgacg gttaagcgaa tcggcaaggg ccattccgac cgttgtttta ccacagcccc
    16981 gaggcccgat cagaaaaaga ggttgtgtca tcgtgggttt tccccaatag gtcgcaatgc
    17041 ggcgaaagcc ggtgtcatga gaatagcgat cataccatca aactagtaca atttcgattg
    17101 taaagaaaaa attccactta aagtgaaaat ctcaatacat cccttactat accaataaat
    17161 attcaagaat gaagtgtaaa taataaatta catttagcca cgactacgtt gcacttccag
    17221 ccaccacttc tccagctccg cggcaaaggc ctggcggtca cgttgtgaaa ggctatctgg
    17281 tccgccggtc tggatcccac tggcacgtaa ggtatccata aaatcgcgca tcgtcaggcg
    17341 ctcacgaatg gtcgctggcg tgtaacgttc gccgcgcgga ttaagcgccg ctgcgccttt
    17401 ctcgatggct tcagcagcca aaggtatatc tgcggtgatc accaaatcgc ccgcttcaca
    17461 ctgccggaca atttcgttat cggcaacgtc gaaacctgcc gcgacgcgca gcgtacgaat
    17521 aaatcgcgat ggcggcacgc gtaaactctg gtttgctacc agtaccagcg gcatctgcat
    17581 acgttccgcc gcgcgataca aaatctcttt aattacattg ggacacgcgt cggcatccac
    17641 ccaaattgtc ataaagtcat cctttgttgg gtaatcctct attgtgtcgc gcttttgcct
    17701 tccggcatag ttctgtttat gcttctgcca gcgattatca aaacaatgaa tttcacggca
    17761 ggagtgaggc aatggaaaag aaaatcggtt ttattggctg cggcaatatg ggaaaagcca
    17821 ttctcggcgg tctgattgcc agcggtcagg tgcttccagg gcaaatctgg gtatacaccc
    17881 cctccccgga taaagtcgcc gccctgcatg accagttcgg catcaacgcc gcagaatcgg
    17941 cgcaagaagt ggcgcaaatc gccgacatca tttttgccgc cgttaaacct ggcatcatga
    18001 ttaaagtgct tagcgaaatc acctccagcc tgaataaaga ctctctggtc gtttctattg
    18061 ctgcaggtgt cacgctcgac cagcttgccc gcgcgctggg ccatgaccgg aaaattattc
    18121 gcgccatgcc gaacactccc gcgctggtta atgccgggat gacctccgta acgccaaacg
    18181 cgctggtaac cccagaagat accgctgatg tgctgaatat tttccgctgc tttggcgaag
    18241 cggaagtaat tgctgagccg atgatccacc cggtggtcgg tgtgagcggt tcttcgccag
    18301 cctacgtatt tatgtttatc gaagcgatgg ccgacgccgc cgtgctgggc gggatgccac
    18361 gcgcccaggc gtataaattt gccgctcagg cggtaatggg ttccgcaaaa atggtgctgg
    18421 aaacgggaga acatccgggg gcactgaaag atatggtctg ctcaccggga ggcaccacca
    18481 ttgaagcggt acgcgtactg gaagagaaag gcttccgtgc cgcagtgatc gaagcgatga
    18541 cgaagtgtat ggaaaaatca gaaaaactca gcaaatcctg atgactttcg ccggacgtca
    18601 ggccgccact tcggtgcggt tacgtccggc tttctttgct ttgtaaagcg ccaaatctgc
    18661 cgatttcaac cactcacgat agtgactcat ttgtgggttc agcggcgcaa cccccacact
    18721 aatccgtaaa gttacctgtg gcgtattcgg caagcgtaac gtgtttagcc cttcacgcac
    18781 ccgtaacatg gcggtaatgg cgctctcagc tggcgtaccg gacatgatta ctgcaaactc
    18841 atcgccgcca aaccgaccaa tcacatcgct accgcgcagg gtaatttgta actgtcgggt
    18901 aagcgccaca atcgcttcat cgcccacatc atgaccccag gtatcgttga tgctcttgaa
    18961 atggtcgata tcgataatca gtaacgttgc atcgcgatta tgccgccgac agttatcaaa
    19021 ttcattgcgt aacatagttt cccaatgacg tcggttatac acgcccgtca tgccgtcgcg
    19081 ggtactcatg acctgcaacc tgcgtttatg ttccgccagt ttggttgccg tctggtagct
    19141 gacccagcca aacagcagag gataaacgac aataatggga agggagagcc accattccag
    19201 cggcgcacta ttgaacgaca cggtaatgcc cgtaagctcg agggtgacaa ggcaggaaac
    19261 caccatcaac accagacccg cgacaaacag acgggggccg cctgccccca tcaaattcag
    19321 acacataatc atcaacatag cggtggaagg cagcacgttt acgcccatta cgcctaccca
    19381 cattcccgct aatactgcat cggtttttaa gttgtaaatt tcccggctaa gcggatcgac
    19441 ggccctgctc gctatctgcc aggctaaatg cggccagacg aacgcccagc cgaccaacac
    19501 cagccaccac cagcccggcg gcgggtgcga aaccagcgtt gaagcaatcg gtaagaacat
    19561 gccagccagg ccaaccgcac ggggtagtct gacgcgacgg gcgaagcgca gcccggaccg
    19621 ctgatgttcg ttttgaggag ttaaaggagg ttcctcccca tgcgccgccg cttttttgaa
    19681 aaagttttca tcattcatta tttttgggaa cattcagaaa caattttccc aaattataga
    19741 gacggggcta aaggcagaag tatgaaattt cgggtgagcc actttcgtag ctcacccgtt
    19801 agcactatca ggtatcaggc ggctttcttg aggcaggcac tcataaactt attacgatca
    19861 tcacctttca gagattgttg cgttgcttga ttattacatt cgcgcatctt ttgctgctgt
    19921 ggcgtcaaac ttttttcacc aggcgcagac ttgctgttct tcaggcaatc actcatgtag
    19981 gtcttacgag catccccttt caacgcctgc gccgtcgcct gctgattaca ggatgtcata
    20041 cgctgttgtt gtggggttaa ggttctctcg gcagcgccga cggtggttaa aaaaaccaga
    20101 ccaaaaagca aggtaaccag taatgttatt ttcatagcac catccctctt catgttttaa
    20161 ccatgagcgt atgcgcccgt gatctgccat taagtctggt tgctaacagc aaaaaaacca
    20221 cccggcagcg aaaattcact gccgggcgcg gttttatttc agccccaggg cggctttcat
    20281 ggtgtagaag agatcggtct ggtcggtcag tccaacaaca ttggcggcat gcgggccata
    20341 cgccgcaata cgcaactgac tgccggtatg ttcttgtgaa tcctcttcgg agttcccgta
    20401 gctcatcacc atcactgcgc catctttggt atttagcgcc tgggtgaggc ccggagcttt
    20461 ggtatccggc gcgacaatct ggctggcgtg ggcgtgatca gcggtgacta tgaccagcgt
    20521 attgccatcc tttttagcga attccagcgc ccgttgtacg gcttcatcga gatcgaccgt
    20581 ctcgccaatt tgcccacaag gattcgcagc gtgatcctgt ttatcgattg acgcaccttc
    20641 aacttgcagg aaaaatcctt tctcattttt actcaacaat tcaatggctt tgtcggtcat
    20701 ctgcgccagg gttggtacac tgtcattacg ttgcggatta ggcgtacagg tgactgcggg
    20761 cttatcgata ttgccgtggt acgttgcttt cggtcctagc cagcgcactg gcatattgcc
    20821 gtcagcaaac agtccaagca gaggtttttg ctgattcgct tccgtcaccg aattcagtga
    20881 ggcagcatcg ctgaccaact gataaccacg cgcctgtgcc tgttcacgca gcgtttttcc
    20941 ctgccattca ccggcggttg ccgtttcagc aaaggttttt gcgccaccgc caagcgtaac
    21001 gtcggcacga gcgttaagca gctgttcggt aatcgatcct tttccgcctt tttccagagc
    21061 gttacccgga catttttcac tggtcgcgct cggaccgtag catttgcgcg aagtcacatg
    21121 tgccaccagc gcagcgggcg tggcatcctg caactctgcg gtagaaacgt taccggtcgc
    21181 cagacctgcg gcttttgcca tttccagaat cgttgggtga tctttttcgt gaatatcgac
    21241 gcccagcgcg ccgttatagg ttttgacacc ggttgaccag gcggttgctg atgcagccga
    21301 atcggtgacg tagtccggtt tgccggtttt tttattcagc gcatagtgag tgtattgccc
    21361 ggtaagcggt aaggcatcga tacctttaaa aaagccgccc gcaccttcgg cataattacg
    21421 tgcggctgta atttccgaat cccccatccc atcgccaatc agcaaaataa tattttttgc
    21481 aggtttatcg ctaagagaat cacgcagagc ggcagtctga tcacccgtta aacggcgagc
    21541 accgccgggt gcagtaatat cgccctgagc agcccggttt tccagaacag gcatttctgg
    21601 tgtccgggct tttgtcacag gggtaaacag taacgataag agtgccagtg caatagtgct
    21661 ttgtttcact ttattttctc catgtacaaa tacattaaaa aataaaaaca aagcgactat
    21721 aagtctcggc cgtgacaact ttatgacagc tgttgaaaag attaactttt tactgacgag
    21781 gatgcttcaa taacttcttt acgtaatcgc gcagcagctc cgtatcgtcg tcaggaatgc
    21841 tggcatcggg ctttacctcg tacagcgccc cctctacctg atcaatcaac cgctgttggt
    21901 cattttgcgc catattgcga agcattgcag tgacgataat ctccaaagct tctacctgcg
    21961 cacacagttc tttcgactct tcttcttttt gggcaagctt aaataacaac tcagcaatga
    22021 gatttttcat gtctgtattt ccttatccaa agtatggaga agttaacatt atgtttcaca
    22081 acttcatagt gtctggaagt attattttta tgacaaagga aatactttgc gcaaatattt
    22141 tttgtttgat taccagcgaa acgtttctct
//
LOCUS       DAZGAN020000074        22027 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGAN020000074 DAZGAN020000000
VERSION     DAZGAN020000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.074"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..287)
                     /locus_tag="ACHKYO_RS15700"
                     /old_locus_tag="pgaptmp_003140"
     CDS             complement(<1..287)
                     /locus_tag="ACHKYO_RS15700"
                     /old_locus_tag="pgaptmp_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4429951.1"
                     /translation="MSGSVIHSQSAAMVPAVYSAGQSPQLPVVIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSL"
     gene            complement(288..593)
                     /gene="ccdB"
                     /locus_tag="ACHKYO_RS15705"
                     /old_locus_tag="pgaptmp_003141"
     CDS             complement(288..593)
                     /gene="ccdB"
                     /locus_tag="ACHKYO_RS15705"
                     /old_locus_tag="pgaptmp_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HGU4429952.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(595..813)
                     /gene="ccdA"
                     /locus_tag="ACHKYO_RS15710"
                     /old_locus_tag="pgaptmp_003142"
     CDS             complement(595..813)
                     /gene="ccdA"
                     /locus_tag="ACHKYO_RS15710"
                     /old_locus_tag="pgaptmp_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HGU4429953.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            1660..2898
                     /locus_tag="ACHKYO_RS15715"
                     /old_locus_tag="pgaptmp_003143"
     CDS             1660..2898
                     /locus_tag="ACHKYO_RS15715"
                     /old_locus_tag="pgaptmp_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004172012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HGU4429954.1"
                     /translation="MEVFMKDVSILNEMIFSASTENELQEKFKDIYHRINNESEYDDS
                     GSVEFLPLPRLNSRSERSEVQDIISVVIESGFFTSGPYIGKVEETLRLFYGAHTCIAT
                     SSGTDALKIALKSVGVEPGDEVIVPLNSFAATENAVMAVGAVPVFANIDSSFNMLPDE
                     VDRLRTSKTKAVLPVCLYGSCMYIDAIHRIARKADIPVIVDAAQCFGIRSLINHCDLL
                     ALSFNPFKNIGSLGKSGAVLTLSPELARLARQYSYHGFAEGKKNIKAQNWGLNSRMDN
                     LQAATLSVKLHHFENNAKKRCLLAARYHLLLSDLSHNIILPTETHQNTWHLFPILLCK
                     GERDSLFAFAREKGVELDIYYPVLSHCGEHPLATGYSRREQFSDSEKIHSALLHLPLH
                     NHMSLQEQNIVIEVLHDYFK"
     gene            2882..3711
                     /locus_tag="ACHKYO_RS15720"
                     /old_locus_tag="pgaptmp_003144"
                     /pseudo
     CDS             2882..3711
                     /locus_tag="ACHKYO_RS15720"
                     /old_locus_tag="pgaptmp_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
     gene            3711..4727
                     /locus_tag="ACHKYO_RS15725"
                     /old_locus_tag="pgaptmp_003145"
     CDS             3711..4727
                     /locus_tag="ACHKYO_RS15725"
                     /old_locus_tag="pgaptmp_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4429955.1"
                     /translation="MIIGIVGCGGIARAHVNALCLNPLVTGLALYDVNPDKVRQLSEI
                     SSLPVTNCQDLEQLAGVSQGFIICTPNHLHVSVAEEVLRYKQLPFVCEKPLSTDLNSA
                     RYLQEIAPKGSIVSFNYRYNPVIDMIMRLRNERKLGELHFFSAEFNKNSALTRHHLTW
                     RDSAQQSKSSGALGDLSCHLLDLFCFIGESPVVVHGIKTVKGTRVGTKSDGQVEVDDN
                     GYVMGSSEKGAYFRVHASKSETDQNLGLHIQLVFENGEIRYSTHHENRLLLILFNDTN
                     TETIGFDALKRLPDPPRELPFWSDSFIHLHDDWCALIKYGHSSPKLADLSSGLHIQEI
                     IEAF"
     gene            4729..4998
                     /locus_tag="ACHKYO_RS15730"
                     /old_locus_tag="pgaptmp_003146"
     CDS             4729..4998
                     /locus_tag="ACHKYO_RS15730"
                     /old_locus_tag="pgaptmp_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HGU4429956.1"
                     /translation="MDTILLTGLFAAFFTTFAFAPQSIKTIRTRNTEGISVVMYIMFL
                     TGVISWIAYGIMRSDFAVLIANIVTLFLAAPVLVITLINRRKKHV"
     gene            4995..6527
                     /locus_tag="ACHKYO_RS15735"
                     /old_locus_tag="pgaptmp_003147"
     CDS             4995..6527
                     /locus_tag="ACHKYO_RS15735"
                     /old_locus_tag="pgaptmp_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4429957.1"
                     /translation="MNIAIIGAGPAGIISARNAIKAGHSVVLFEKNTRIGGIWTPWSG
                     GAYRNACMQNSRYTFHYTGFPPGDIDEFPGVEQVFRYLSAVAGEDALRESTRLNTEVV
                     SLRKDAGHWVIRCASEGKDTEDIFDRVIIATGELWQPRRPPLPGEENFSGTLITSRDY
                     QEPEAFKGKNILIIGGGVSGADIASDLVPFARSVSLSVKKMGLYLPRQFPTGPNDMMH
                     SYLGRCLLSQMNYEDFIGYLDTMMPDYMQAYRASGLLPDMANNNAVHVNEKIIPNVAA
                     GLIKVKPLAERFTGEGAIEFVDASQEKYDVIITCTGYEMPDYSFIQGFDRTQLYEHFF
                     WTEDPSLAVINPPVDTAGFGAAFPYFDIISQWVMNVFSGKTSLPEKEAMRKWCAEHMA
                     SLHVKRFYDSWLETIRIGLLSGLLPDPARDFSRYWNIISSMVKPAYLATPPAFPEHGM
                     MDSLFDFRIARIRILSGLGNDALGYLLKKGDITDAEYRAALEIDPRQSISVHLPYSQT
                     YL"
     gene            complement(6560..7012)
                     /locus_tag="ACHKYO_RS15740"
                     /old_locus_tag="pgaptmp_003148"
     CDS             complement(6560..7012)
                     /locus_tag="ACHKYO_RS15740"
                     /old_locus_tag="pgaptmp_003148"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGU4429958.1"
                     /translation="MKFMECAVRDVIYGTNVRIVKPVNIYECELRDNVFVGPFVEIQK
                     GCVIGSGSRIQSHTFICENVTLGENCFIGHNVTFANDLFRSGAPDPSPDNWISIILGD
                     SVTVGSGAIILSPYICSGAVIGAGAVVVKPVENKGIYAGNPARLIRIL"
     gene            7341..7598
                     /locus_tag="ACHKYO_RS15745"
                     /old_locus_tag="pgaptmp_003149"
     CDS             7341..7598
                     /locus_tag="ACHKYO_RS15745"
                     /old_locus_tag="pgaptmp_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429959.1"
                     /translation="MDQEMTFSLSYEQLTRFAERRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRKLIWPEDDR"
     gene            7598..8188
                     /locus_tag="ACHKYO_RS15750"
                     /old_locus_tag="pgaptmp_003150"
     CDS             7598..8188
                     /locus_tag="ACHKYO_RS15750"
                     /old_locus_tag="pgaptmp_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429960.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEEAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAERR
                     AAEVVVLPLVPATGLPADGTSATDLAVELNGMTYQACRGDFVVRLDGSTCLQLWNKEG
                     KVVRREGDPLEVAQWLQACHDAGMEVRVQVNESITP"
     gene            complement(8206..8424)
                     /locus_tag="ACHKYO_RS15755"
                     /old_locus_tag="pgaptmp_003151"
                     /pseudo
     CDS             complement(8206..8424)
                     /locus_tag="ACHKYO_RS15755"
                     /old_locus_tag="pgaptmp_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
     gene            8582..8782
                     /locus_tag="ACHKYO_RS15760"
                     /old_locus_tag="pgaptmp_003152"
     CDS             8582..8782
                     /locus_tag="ACHKYO_RS15760"
                     /old_locus_tag="pgaptmp_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429961.1"
                     /translation="MKLDISVKYFLNIMIADFIIPAPEKISTTWHSDTKMNRFACPIH
                     STNSDYYSGFYHTDVLIPGELA"
     gene            8822..9004
                     /locus_tag="ACHKYO_RS15765"
                     /old_locus_tag="pgaptmp_003153"
     CDS             8822..9004
                     /locus_tag="ACHKYO_RS15765"
                     /old_locus_tag="pgaptmp_003153"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429962.1"
                     /translation="MIFNWNVLFYVSLEKFPVGNPYYGEIPSIPVDEEAKFFNPFPPR
                     AFENKPLYTEEITKYD"
     gene            8997..9863
                     /locus_tag="ACHKYO_RS15770"
                     /old_locus_tag="pgaptmp_003154"
     CDS             8997..9863
                     /locus_tag="ACHKYO_RS15770"
                     /old_locus_tag="pgaptmp_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4429963.1"
                     /translation="MIKGIMFAFLAAFSWGAAIVMSKKGLENMDAGELFFWQVGSAAL
                     LSWFVLAISRKKLPVTKKSTLAYSTGIFEPFLAYTFTLYGLKFISAGITSVIFSLESV
                     FILILSVIFFSVKINSLLHFILLLTGAMAGSIMVALPDINDRIDNITGYILIVAGVLS
                     ASFYVVISSRLIVNFEPVTLLTGQLTFSFILACFFIVLTDTSLQLPIESVFLVFLSGI
                     LQYFLAFCFYLYSLRWIPVHIAGAMLYFIPLVALLLSWFFLQEDISFIQGLGVFITIL
                     CVYILNKKYAIE"
     gene            10104..10289
                     /locus_tag="ACHKYO_RS15775"
                     /old_locus_tag="pgaptmp_003155"
     CDS             10104..10289
                     /locus_tag="ACHKYO_RS15775"
                     /old_locus_tag="pgaptmp_003155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429964.1"
                     /translation="MLTQLHRVLYRCPGHYVYGSATQSTGTGTGSLASRNTSQSPLPA
                     QQAGFPRPELYSLSVCQ"
     gene            10347..10952
                     /locus_tag="ACHKYO_RS15780"
                     /old_locus_tag="pgaptmp_003156"
     CDS             10347..10952
                     /locus_tag="ACHKYO_RS15780"
                     /old_locus_tag="pgaptmp_003156"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4429965.1"
                     /translation="MTLYQIKPSFQALLRPLMFWLNRRGVTANHITLCAMVLSLITGV
                     VLIIHPQPGLFVLLPVVLFVRMALNALDGMLARECNQRSRLGAILNETGDVISDISLY
                     LPFMLLPQSSIPLVLIVVFCALMTEFCGVLAQTINGVRSYAGPLGKSDRALVFGAWGL
                     SLSIWPQIVAWNNVIWSVATLLLLWTIFNRCRSALREEGVK"
     gene            10949..11866
                     /locus_tag="ACHKYO_RS15785"
                     /old_locus_tag="pgaptmp_003157"
     CDS             10949..11866
                     /locus_tag="ACHKYO_RS15785"
                     /old_locus_tag="pgaptmp_003157"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4429966.1"
                     /translation="MTLLEKSLVVIFSLLLIATVINGLLVWLRPGKDWRELTLRIRTW
                     WVIIVLFSFALLSPSWLALTFFALVSFMALKEFLTLVPSRQSDRMPLLWMFIAIPLNY
                     WLIGIGWYGMFVVFIPVYVFLFLPARMVIAGDTQGFLRTVSQLHWSLMTTVFAFSHVA
                     FLLVLPADGMQTSALLVLFLVGLTEFNDIAQYLWGKSLGRIKVIPKVSPNKTLEGLLG
                     GVATTALAATVLGPLLTPLSWPMALLAGVIIGVTGFCGDVVMSAIKRDIGVKDSGTLL
                     PGHGGILDRLDSLIFTAPVFFHFVRYFCY"
     gene            11870..12511
                     /locus_tag="ACHKYO_RS15790"
                     /old_locus_tag="pgaptmp_003158"
     CDS             11870..12511
                     /locus_tag="ACHKYO_RS15790"
                     /old_locus_tag="pgaptmp_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HGU4429967.1"
                     /translation="MMTRLLRTLFTLCIVWPVIWLWLGLRIRHRERLPKHGPSIIVAN
                     HNSHMDVFALLSLFPLRYQASVHPVAAADYFLRDKRMAWFALKILNIIPISRKGGYTD
                     PLAQCEQALREGKTLILFPEGTRGEPGVLAPLKSGVWHLSQRMPEVPIIPVGLRGTEH
                     VMAKGNRIPLPLFIDVNIGDALSTHPEKKTFMDDLEQRLTALLQPSQGNHPHD"
     gene            12504..14264
                     /locus_tag="ACHKYO_RS15795"
                     /old_locus_tag="pgaptmp_003159"
     CDS             12504..14264
                     /locus_tag="ACHKYO_RS15795"
                     /old_locus_tag="pgaptmp_003159"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HGU4429968.1"
                     /translation="MTNSRIPDERQFLTSDNTELFYRHWPAQEASSTPKVVVLFHRGH
                     EHSGRLQHIVDELAMPDTAFYAMDARGHGRSPGLRGYSPSLVRSVQDVDEFVRFAAAD
                     SQAGMTDVVVVAQSVGAVLAATWAHDYAPDIRGLVLASPAFKVKLYVPFARQGLELMY
                     RLRGLFFVNSYVKGKYLTHDPERVASFNKDPLITRPIAVNILLDLYKTAERVVSDASA
                     ITLPVQLLVSGEDYVVHRQPQLDFYQNLRNPQKELHVLPGFYHDTLGEKERHLAFDRM
                     RSFIDTLYARPVQRFDYSQEDRWSPGADTWRILSGGPALYSLDDISYRCLRYGMKVLG
                     TQSTGVRLGFETGFDSGSTLDYIYRNQPQGSTSLGRLIDKNYLNSVGWKGIRQRKIHL
                     QMLIQQAVARLTEQGSAVRIVDIAAGHGRYVLDALQGETAVSEILLRDYSELNVAKGQ
                     EMIASRGMAGRARFEQGDAFNRNELAALEPRPTLGIVSGLYELFPENGPVRDSLAGLA
                     AAIEPGGILIYTCQPWHPQLKTIAWSLTSHKNGKAWVMRVRTQGEMDALVRDAGFEKC
                     TQLIDEMGIFTVSMAVRRQP"
     gene            14261..14443
                     /locus_tag="ACHKYO_RS15800"
                     /old_locus_tag="pgaptmp_003160"
                     /pseudo
     CDS             14261..14443
                     /locus_tag="ACHKYO_RS15800"
                     /old_locus_tag="pgaptmp_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206187.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14476..15956)
                     /locus_tag="ACHKYO_RS15805"
                     /old_locus_tag="pgaptmp_003161"
                     /pseudo
     CDS             complement(14476..15956)
                     /locus_tag="ACHKYO_RS15805"
                     /old_locus_tag="pgaptmp_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(15987..16337)
                     /gene="tnpB"
                     /locus_tag="ACHKYO_RS15810"
                     /old_locus_tag="pgaptmp_003162"
     CDS             complement(15987..16337)
                     /gene="tnpB"
                     /locus_tag="ACHKYO_RS15810"
                     /old_locus_tag="pgaptmp_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4429969.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRRNSLTML"
     gene            complement(16334..16696)
                     /locus_tag="ACHKYO_RS15815"
                     /old_locus_tag="pgaptmp_003163"
     CDS             complement(16334..16696)
                     /locus_tag="ACHKYO_RS15815"
                     /old_locus_tag="pgaptmp_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4429970.1"
                     /translation="MVKLALQPGASVARIAREHDINDNLLFKWLRLWQNEGRVSRRLP
                     VTTSSDTGVELLPVEITPDEQKEPVVAIAPSLSTTTQTRVSASSCKVEFRHGNMTLEN
                     PSPELLTVLIRELTGRGR"
     gene            16916..17341
                     /gene="nudI"
                     /locus_tag="ACHKYO_RS15820"
                     /old_locus_tag="pgaptmp_003164"
     CDS             16916..17341
                     /gene="nudI"
                     /locus_tag="ACHKYO_RS15820"
                     /old_locus_tag="pgaptmp_003164"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HGU4429971.1"
                     /translation="MRQRVIVCPVIQNEGEFLLCKMASDRGVFPGQWALPGGGMEPGE
                     TMETALRREIKEELGKELHITDVKPWAFRDDIRTKTYPDGTHEDIYMIYLIFDCISTN
                     RTVTFNEEFQGIVWASPDTLKYMDLNEATRITFSQKGIL"
     gene            17399..17565
                     /locus_tag="ACHKYO_RS15825"
                     /old_locus_tag="pgaptmp_003165"
                     /pseudo
     CDS             17399..17565
                     /locus_tag="ACHKYO_RS15825"
                     /old_locus_tag="pgaptmp_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            18023..18166
                     /locus_tag="ACHKYO_RS15830"
                     /old_locus_tag="pgaptmp_003166"
                     /pseudo
     CDS             18023..18166
                     /locus_tag="ACHKYO_RS15830"
                     /old_locus_tag="pgaptmp_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            18238..18510
                     /locus_tag="ACHKYO_RS15835"
                     /old_locus_tag="pgaptmp_003167"
     CDS             18238..18510
                     /locus_tag="ACHKYO_RS15835"
                     /old_locus_tag="pgaptmp_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429972.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFADFIAGHSSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            18491..19697
                     /locus_tag="ACHKYO_RS15840"
                     /old_locus_tag="pgaptmp_003168"
                     /pseudo
     CDS             18491..19697
                     /locus_tag="ACHKYO_RS15840"
                     /old_locus_tag="pgaptmp_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            20160..20384
                     /locus_tag="ACHKYO_RS15845"
                     /old_locus_tag="pgaptmp_003169"
     CDS             20160..20384
                     /locus_tag="ACHKYO_RS15845"
                     /old_locus_tag="pgaptmp_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGU4429973.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
     gene            20381..20767
                     /locus_tag="ACHKYO_RS15850"
                     /old_locus_tag="pgaptmp_003170"
     CDS             20381..20767
                     /locus_tag="ACHKYO_RS15850"
                     /old_locus_tag="pgaptmp_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HGU4429974.1"
                     /translation="MKFVSSQEVIEFHDRLISRDGGVAGMTEPGRADAIIHRVLNMYH
                     YEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVAQVFLKRNGVHIISSRISFDEMQ
                     IIALNAATGEYNWKMVSDHLKAIILN"
     gene            complement(21051..21204)
                     /locus_tag="ACHKYO_RS15855"
                     /old_locus_tag="pgaptmp_003171"
                     /pseudo
     CDS             complement(21051..21204)
                     /locus_tag="ACHKYO_RS15855"
                     /old_locus_tag="pgaptmp_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312755.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(21313..21594)
                     /locus_tag="ACHKYO_RS15860"
                     /old_locus_tag="pgaptmp_003172"
     CDS             complement(21313..21594)
                     /locus_tag="ACHKYO_RS15860"
                     /old_locus_tag="pgaptmp_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4429975.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLSERLENPRVPASRL
                     HGHADRYKIKLRTSGYRLVYQVIDEKVVLLVISVGKREGNEVYQIADMR"
     gene            complement(21584..21835)
                     /locus_tag="ACHKYO_RS15865"
                     /old_locus_tag="pgaptmp_003173"
     CDS             complement(21584..21835)
                     /locus_tag="ACHKYO_RS15865"
                     /old_locus_tag="pgaptmp_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGU4429976.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVVILNRNEPAFYC
                     VPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
BASE COUNT         5491 a         5345 c         5454 g         5737 t
ORIGIN      
        1 agcgaaaaat ccccccgcgt cagcgccagt gcttcattaa tgcgcgcacc ggtgttccac
       61 agtgtggcca acagcatctt gcggcgcaga tccgggacgt aatggagcag ggcactcact
      121 tccggagcca gcagatattt cggcagttca tcatgaacca tcgacatctg gcgaagtgcc
      181 agagctgccg gataatcaat gacaacagga agttgtggag actgtccggc agaatacact
      241 gccggtacca tggctgcaga ctggctgtgt ataacggagc ctgacattta tattccccag
      301 aacatcaggt taatggcatt tttgatgtca ttttcccggt ggctgagatc agccacttct
      361 tccccgataa cggagaccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca
      421 tccccgacat gcaccaccgg gtaaagttca cgggagactt tatctgacag caggcgggca
      481 ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa taatgtcact ctggacatcc
      541 acaaacagac gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca
      601 gtccctgttc tcatcggcaa aagagccatt catttcaata aacctggcga cctctgccat
      661 cccttcctga ttttcagcct gccagcgttc agcacgcagt cgccgggctt cgttctgcat
      721 ggtcgtactg accagaccgg agatattcac gtcatacgcc ttgagcagct gatagctgtc
      781 gctgtcaact gtcactgtaa tacgctgctt catatcacac ctctttttaa catacttctg
      841 gtatgcatat cagtatatat tcttatgccg caaaaatcag cgcgttaata cacatactgt
      901 tatcttgctt ttagtaagcc ttatttattt taccttgcat tatgttaacc aataaaaatt
      961 aaaatcagac ttataaaaac aaagcgtaat tatcgaagga ccatttccgg cagatccgat
     1021 ttttctgtta ttgacgggtt tccggaagtg taccacgctg aggaaatcat catatcgctg
     1081 ttttccgccc ctgcgtggcg catcacaggt cagggaaata acacaacata tagtgtcatg
     1141 agcagccgaa tgaagcacta catgttgtac ttacaggagg tggcgttgtg tgggggagag
     1201 gaagatccgt gaccaggggt tttcggatct ctggagcaga aaagaaaatt attgccagat
     1261 aatcacaaaa aatggtggat tattgtgatg aagtgattat tgcaatcaga acaataactg
     1321 gaattgtaaa gatcatattc atcaataaat agcagagtca cagatctgct gaaagccaaa
     1381 ccatgaagat actacccgag tccgcttctg gaagatccat ttcaaatatc cggatacaca
     1441 ggcaactgtg gcgacgattg taatgacatt aaccatatcg gcttccctga agcattggtt
     1501 atggcaacca cgttatcaat atctttccat cattttcagt aaatatcact aacagaccga
     1561 tattaccgga cctgtaagtt atttgtgact gatatcacca atttgtaaat caaatgtgaa
     1621 aatatggttt atctgtgctt tatttttccc cgacataaaa tggaggtctt tatgaaagat
     1681 gtcagtattt tgaatgaaat gatcttcagc gcatcaacag aaaatgaact acaggaaaaa
     1741 tttaaagata tatatcaccg tatcaacaac gagagtgaat atgatgattc tggttcagtc
     1801 gaatttctgc cacttccaag actgaattcc cgttctgaac gctcagaagt acaggacatc
     1861 atttcagttg taattgaaag cggatttttt accagtggcc cctatattgg aaaagtagaa
     1921 gagacattaa gattatttta tggtgctcat acctgtattg ccaccagtag cggcaccgat
     1981 gcgttaaaaa tagctcttaa atctgttgga gtggaacccg gagatgaggt catcgttccg
     2041 ctgaactcct ttgcagctac ggagaatgca gtgatggccg tgggagccgt tccggtcttc
     2101 gctaatattg acagttcttt caatatgctt ccggatgaag tggatcgcct caggacttca
     2161 aagacaaaag ccgtgttgcc ggtctgcctt tatggctcat gcatgtacat tgatgccatt
     2221 caccggattg cccgcaaagc cgacattcca gttatcgtgg atgcagcaca gtgctttggt
     2281 atcaggtcac tcatcaacca ctgtgatctg ctggcgttga gctttaatcc attcaagaat
     2341 attggttctc ttggtaaatc cggtgcagtt ctaacccttt ctcctgaact ggcacgcctg
     2401 gcaaggcaat attcatatca cggatttgct gaaggtaaaa agaatattaa ggcccagaac
     2461 tggggattaa acagccgtat ggataatctg caggctgcca cgcttagtgt caaacttcac
     2521 cattttgaaa ataatgcaaa aaaacgctgc cttcttgcag cccgttacca tcttttactg
     2581 tctgacctgt cacataacat aatacttccg acagagaccc atcagaatac atggcacctt
     2641 ttccctattc tgctctgcaa aggcgaacgt gattctctgt tcgcctttgc tcgtgaaaag
     2701 ggagtggagt tagatattta ctatcccgtc ctttcacatt gcggagagca tccattggca
     2761 accggatatt cccgtcgaga gcagttcagt gattctgaaa aaatacattc tgcattactt
     2821 catctgccgc tgcataacca tatgtcgctc caggagcaaa acatcgttat cgaggtactg
     2881 catgactatt ttaaataaag actggcccgt cgaattcccg ttcccgacag taatccgcac
     2941 tgtggtgtgc agcgatcttg atgaaactta tattccttcg gacagcgata aaaaaaactg
     3001 agtgggatcg ttcagctgga atcctatatt acatcccatg ccgaaaaaaa ggggattctc
     3061 gctggctgga tcacaggaac aaatctggat tcagcctggc gaaaatccag gggatatata
     3121 tcgcgaagcc ctcactttat atgttgcagt ctcggcaccg aattttactg ggtgaaaaat
     3181 ggtcgtctca tcccctctga aacctgggca gaaagaataa gccgttcagg ctacagtcgt
     3241 cagaatgtca actgtctggt taagattctg atggaaaaag agatccccct tctgaaacaa
     3301 ccagaggatt atcagggccc ctttaaagtg agctattact accctgagag cccatctatt
     3361 gccagcgatt ttgccaccat ccaggaactg gctgacgagc gacgaattcg tgttgtgttt
     3421 acccgctgta acccggcggc tggcgatccc tctggctgtt atgacgttgt atttatcccc
     3481 ctctgttgtg gcaaggatca ggccgtgtct tttctgatgg aggaaaccgg gctgccgaaa
     3541 gaagccatta ttgcctttgg tgacagtgcg aatgattttg ccatgtttgc acaggccgga
     3601 agtggctatc tggtgggtaa tgccgacccg tttgcagtca gccagtacgg aaacagactg
     3661 aacagtgctt actgccacgg gatactgagc gttcttaagg agctacagtg atgattatcg
     3721 gaattgtcgg ctgtgggggg attgcccgtg cccatgtcaa cgcactttgt ctcaatccgc
     3781 tagtaactgg tctggccctg tacgatgtga atccagacaa agtccgacag ctcagcgaaa
     3841 taagctccct gccggtaaca aactgccagg atctggagca gcttgccgga gtcagtcaag
     3901 ggttcattat ctgcacacca aaccatctcc acgttagcgt ggcagaagaa gtgcttcgct
     3961 acaaacagct cccgtttgtg tgtgaaaaac ccctctcaac agatctcaac tctgcacgat
     4021 atctacagga gattgctcca aaaggtagta tcgtgtcttt taactaccga tataaccccg
     4081 tcatcgacat gataatgcgt ctgagaaatg agaggaaatt aggtgaactg catttcttct
     4141 cagcagaatt taataaaaat tctgcactga cccgccatca cctgacctgg cgagactccg
     4201 cacaacagag caaaagcagc ggtgctctgg gcgatctatc ctgtcatctg cttgatttat
     4261 tttgttttat aggggaaagc ccggtagtgg ttcatggcat caaaacggta aaggggacac
     4321 gggtgggtac aaaatcagat ggtcaggttg aagtggatga taacggctat gtaatgggca
     4381 gttcagaaaa gggagcttat ttcagagttc atgccagtaa atcggaaaca gatcagaatt
     4441 tgggactgca tatacagctt gtatttgaaa atggtgaaat cagatattca acccaccatg
     4501 aaaatcgcct gctcctgatt ctatttaatg atacgaatac ggaaacaatt ggcttcgacg
     4561 ctctgaaacg cttgcctgat ccaccacggg agcttccatt ctggtctgat tcgtttattc
     4621 atcttcatga cgactggtgt gctcttatta aatatggtca ctcgtctcct aagctggcgg
     4681 acctgtcatc cggccttcat atacaggaaa taattgaggc attctgatat ggatacaatt
     4741 cttttaaccg ggctgtttgc tgcatttttc acaacgtttg cgttcgctcc ccagagcata
     4801 aaaactatca ggacacgcaa taccgaaggt atctccgttg ttatgtatat catgttcctg
     4861 acaggtgtaa tctcatggat agcgtatggc attatgcgat ctgattttgc cgtactgatc
     4921 gccaatattg tcacactgtt tctggctgca ccagtgcttg taataacact tatcaaccgc
     4981 aggaaaaaac acgtatgaat attgcaatca tcggcgcggg tcctgccggc atcatttctg
     5041 cccgtaatgc gattaaagca ggtcactccg tggttctgtt tgagaagaat acccgaattg
     5101 gggggatctg gaccccctgg agtggtggcg cttaccgtaa tgcctgtatg cagaactccc
     5161 gttatacatt tcattatact ggctttcccc ctggcgatat cgatgaattt ccaggagtgg
     5221 aacaggtatt cagatatctt tcggccgtag ccggagagga tgccctccgt gagtcgaccc
     5281 gactgaatac tgaagttgtt tcactcagaa aagacgccgg acactgggtg atccgctgcg
     5341 cttctgaagg aaaagacacg gaagacattt ttgaccgggt catcattgca acaggtgaac
     5401 tctggcaacc ccgccggccc cccctgccag gtgaggaaaa cttctccgga acgttgatca
     5461 cgtcgagaga ttatcaggag ccagaggcat ttaaaggaaa aaatatcctc atcattggcg
     5521 gcggtgtcag cggtgcggat attgcctcag accttgttcc ctttgccaga agcgtaagtc
     5581 tgtccgtcaa aaagatggga ctttatctgc cgagacaatt cccgactggc ccgaatgaca
     5641 tgatgcactc ctatctgggc aggtgtctgc tgagccaaat gaattatgaa gattttatcg
     5701 gttatctcga caccatgatg cctgactaca tgcaggccta ccgagcatcc ggtctgttgc
     5761 ccgacatggc gaacaataac gcggtacatg ttaacgaaaa aatcataccg aatgtagccg
     5821 ccggtctgat aaaagttaaa cccctggcag agcgtttcac cggagaaggc gctattgagt
     5881 tcgtcgacgc atctcaggaa aaatatgatg taatcataac ctgtacaggc tatgaaatgc
     5941 ctgattactc gttcattcag ggatttgaca gaacacagct ctatgagcac tttttctgga
     6001 cagaggaccc ttctctggcc gtcattaatc ccccggtgga taccgccggt ttcggggctg
     6061 cgtttcccta ttttgatatc atcagccagt gggtaatgaa cgtattctcc gggaagactt
     6121 cccttccgga aaaagaagct atgcgcaaat ggtgtgctga gcacatggct tcacttcatg
     6181 ttaagcgatt ttatgacagc tggctggaaa ccatccggat tgggctgctg tcaggtcttc
     6241 tcccggaccc ggcccgcgac ttttcccgat actggaacat catcagcagt atggtcaaac
     6301 ctgcctatct ggccactcct cccgcatttc cggagcatgg tatgatggac agcctgtttg
     6361 atttccggat cgccagaata cgcatactgt cagggctggg aaacgatgct ctcggttatt
     6421 tactgaaaaa aggtgatatc actgacgcag aataccgggc ggccctggaa atcgatcccc
     6481 ggcagtcgat ttctgtacac ctgccgtatt cacagaccta cctctgataa aaagggccag
     6541 taacggccct tttctttttt cataatattc tgatcagtct tgccgggttt cctgcataaa
     6601 tgcctttgtt ctcaaccggt tttactacaa ctgcccccgc accgataaca gctccgctgc
     6661 atatatacgg agacagaata atggctccac tgccaacagt aaccgaatcc cccaggataa
     6721 tgctgatcca gttatccggt gacggatctg gtgctcctga acgaaacaga tcgttggcga
     6781 acgtcacgtt atgaccaatg aagcagttct cccccagtgt tacgttctca caaataaacg
     6841 tgtgcgactg tatccgactt ccactaccaa tgacacagcc tttctgtatc tcaacgaagg
     6901 ggccgacaaa cacattgtcc ctgagttcac attcataaat gttgaccggt ttgacaatac
     6961 ggacattggt cccataaatc acatcccgga cagcacattc cataaatttc atactcacac
     7021 gtcctttaaa tacagatgac acttgccgga attatacagc ccagattcag ttgccatgtt
     7081 tcagaatagc ttataagccg cacttttacc gatactcagc aacaaatcag gtctggatcc
     7141 acttcagccg gaaaccggat ctgcgcggca gaaaagacgc aaatgatatg ataattaaga
     7201 aatatacata aaatcatagc agatatgatt ttcatcatta aaaatgaaac aatatctgat
     7261 attatcagaa atcaaaagca caaaaatcat acatgatatt atttttacct gtcagagcat
     7321 tatcagatgg agcgtgcaga atggatcagg aaatgacgtt ttcactcagc tatgaacaac
     7381 tgacccggtt tgcggaacga cgcatccggg aatgcaatct ggacagccag ggcgcgattt
     7441 atctctgcga gtcagccaaa gccggagccg tgctgatctt ctggcatgaa ctggccataa
     7501 atggctatgc ctcaatgaat gccataaaga gacaggagct cattgacgcc gactttcagc
     7561 gcctcagaaa gctcatctgg ccggaggatg acaggtaatg acccagagcc gccgaccgtc
     7621 gccactgcag cggagagtgc tgattgtgct ggctgcgctt gatgaaaaac ggccgggccc
     7681 cgtgctgacg cgggacatag aacgggtgct ggagcgaagc ggggaagccc cggtctacgg
     7741 gccaaacctg cgcgcctcct gccgtcgtct ggaggaggcc ggctggctgc gtaccctgcg
     7801 cgccccaaac ctgcagctgg cagtagagct gacggacgcc ggcagggccg tggcacagcc
     7861 tctgctgctg gcagaacagg accgcctccg ggcagaacgg cgtgcggcag aagtcgtcgt
     7921 actgccgctg gtcccggcaa caggtctgcc cgcagacggc acttctgcca ctgatctggc
     7981 ggtggagctg aacggcatga cgtatcaggc ctgccgtggg gattttgtgg tacgtctcga
     8041 cggcagcacc tgtctgcagt tatggaataa agaaggcaag gtggtacgcc gggagggcga
     8101 tccgctggaa gtggcgcagt ggctgcaggc ctgtcacgat gcaggaatgg aggttcgtgt
     8161 gcaggttaat gaaagcatca ccccgtaaaa acatgcctgc acggtttaca aatcatccca
     8221 tggtggcagc cggttaactt tcctgcgcca ccagtagata aaccacattg cacatcccca
     8281 gaaaatgccc tataagctcc ccatcaacag cgtgacctgc cagaacggca taaagggtaa
     8341 tggcagaagc cggataccca gccgccagag aatacgcaga agaggagggg cataattact
     8401 ccgccacatc tttttgctgt ccatcagggc aatggcccgg gcttttttct actcaaaggt
     8461 cacaggagca cctccgttcc atgatgtaaa cgtgacacag cttattgtta ttgattatta
     8521 ttatcatgct gtgattatgt tatcacgcac cggtacacga taacaataaa ggagaaacca
     8581 gatgaaactg gatatatctg taaagtattt tctgaatatt atgatagcag atttcatcat
     8641 tcccgcacca gaaaaaatat caaccacatg gcactcagat actaaaatga atcgctttgc
     8701 ttgccctata cattctacta atagtgatta ctattcaggt ttttatcata cagatgtatt
     8761 aattcccgga gaactggctt aggacgccat cataatccat gatgttgata gtaatgatcc
     8821 tatgattttt aactggaatg tattgtttta tgtctcactg gaaaagtttc ctgttggcaa
     8881 tccttattat ggggaaattc caagtattcc tgtggatgaa gaagcaaagt tttttaatcc
     8941 atttccacca agagcgtttg aaaataaacc tctttatact gaggagataa ctaaatatga
     9001 ttaagggaat tatgtttgca tttcttgctg ctttttcatg gggagcagca atagtgatga
     9061 gcaaaaaagg tctcgaaaac atggatgccg gagagttgtt tttctggcaa gttggtagtg
     9121 ctgcactgtt atcatggttt gttctcgcaa ttagcagaaa aaaactcccg gtaacaaaaa
     9181 aatcaacact tgcctattca acaggaattt ttgagccatt tctggcgtat acattcactc
     9241 tctatggcct aaaatttatt tctgctggta taacatccgt tattttttca cttgagtcag
     9301 tatttattct tatactatct gttatattct tttccgttaa aattaactct ctattacact
     9361 ttatattatt gcttactgga gcaatggcag gcagtataat ggttgcccta cctgatatca
     9421 atgaccgcat tgataatatt acaggatata tacttattgt tgcaggtgta ctctcagcat
     9481 ccttttatgt tgttatctca tcacgactta ttgtgaattt tgagcctgtt acgttgttaa
     9541 ctgggcagtt gactttctct tttattcttg cttgtttttt tattgttcta actgacacat
     9601 ccctgcaact gccaatagaa tctgtatttc ttgtgttttt gtcaggaatt ttacaatatt
     9661 ttttggcttt ttgtttctat ctttattccc tacgctggat tcccgttcac atagcaggag
     9721 caatgctcta ttttattcct ctggtagcat tgctgttgtc ctggttcttc ctgcaggaag
     9781 atatatcatt tattcaggga ctgggcgttt ttataactat tttatgcgta tatattctca
     9841 ataagaaata cgccatcgaa tgactttatt agggtcgcgt tatcgcggta gggtacaccc
     9901 tgacaccata accaaaaacg tgatgccaga cgatgaatga cataacctta atgcgataaa
     9961 gtggtaaaaa atgttcatca gagctgattt aaaagaagta aagcaatagc ccggaccaga
    10021 gacaaggcat catgcaacaa atgtaacgga tattttgaaa tccttgcctc cgcacatgac
    10081 tgggcataat taaagaaaag cgtatgctga cacagctgca cagagtgctg taccgttgtc
    10141 caggacacta tgtttatggt tcagccacac agtctaccgg cacaggtact ggctctctgg
    10201 cctcccgtaa taccagtcag tcgcctttac cggcgcagca ggcaggattt ccgcgcccgg
    10261 aactgtattc actttcagtg tgccagtgac agccccaata aaaggccaga ctcaaccccg
    10321 tcaaatatgc ttattaagga gtgatgatga ccctttacca gataaagccc tcgtttcagg
    10381 cgctgttaag accgctgatg ttctggctga acaggcgcgg cgtcaccgct aatcacatta
    10441 cactgtgcgc gatggtgctt tcccttatca ccggcgtggt gctcattatt catcctcagc
    10501 ccggattatt cgtccttctg cccgtggtgt tgtttgtacg aatggcgctc aatgcgctcg
    10561 atggtatgct ggcgcgtgaa tgtaatcaaa gatcgcgtct gggggccatc ctcaatgaga
    10621 cgggtgatgt aatttccgac atctcgcttt atttgccctt tatgctactc ccgcagagca
    10681 gtataccgct ggtgctgatc gtggtgtttt gtgcgctgat gaccgagttt tgcggcgttc
    10741 tggcgcaaac gattaacggc gtgcgcagct atgccggtcc gctgggcaaa agcgacagag
    10801 cgctggtgtt tggtgcatgg gggctgtcac tgtcgatctg gccgcagatt gtcgcctgga
    10861 ataatgtcat atggagtgtc gcaacactgt tgttgttatg gacgattttt aatcgctgtc
    10921 gcagcgcatt gcgtgaagag ggtgtgaaat gacgctactt gaaaaatcac tggtcgttat
    10981 tttttccctg ctgttgattg ccaccgtaat caacgggttg ctggtgtggc tgcgtccagg
    11041 taaagactgg cgcgagctga cattacgtat tcgcacctgg tgggtgatta tcgtcctgtt
    11101 ttccttcgca ctattaagtc ctagctggct ggcactgacg ttcttcgcgc tggtcagctt
    11161 tatggcgcta aaagagtttt tgacgctggt gccttcccgc cagtccgacc gcatgccgct
    11221 gttatggatg tttattgcaa ttccccttaa ctactggctg ataggtatcg gctggtacgg
    11281 catgtttgtg gtgtttatcc cggtctacgt gttccttttt ttaccggcgc gaatggtgat
    11341 tgctggggat acacagggct ttttacgtac cgtttcccag ttgcactgga gtctgatgac
    11401 caccgtattt gccttcagcc atgtcgcgtt tttactggtg ttaccggcgg atggaatgca
    11461 aaccagcgcg ctgttggtgc tgtttttggt cggcctgacc gaatttaacg acatcgcgca
    11521 atacctgtgg ggcaagtcgc ttggtcgtat aaaagtcata ccgaaggtca gcccaaacaa
    11581 aaccctggag ggattgctgg gtggggtggc aacgacggcg ctggcggcaa cagtcctggg
    11641 gccactgttg actcccctga gctggccgat ggcactgtta gccggggtta ttatcggcgt
    11701 tactggcttt tgcggcgacg tggtgatgtc ggcaatcaag cgtgatatcg gcgtcaaaga
    11761 cagtggtacg ctgctacccg gccatggagg catccttgat cggctggatt cgctgatttt
    11821 taccgcaccg gtcttcttcc attttgttcg ctatttctgc tactaaccta tgatgacacg
    11881 actactccgt accctgttta cgctgtgtat cgtctggccg gtcatctggc tgtggcttgg
    11941 tctgcgtatc cgccaccgcg agcgactgcc gaagcacggg ccttcgataa tcgtggcaaa
    12001 tcacaacagc catatggatg ttttcgcctt gctttcgctt tttccgctgc gttaccaggc
    12061 ttctgttcat ccggttgctg ccgcggatta ttttctgcgg gataaacgaa tggcttggtt
    12121 tgcgctgaag atccttaaca tcattcctat ttcaagaaag ggcggttata ccgatccgct
    12181 ggcgcaatgt gaacaggcgc tgcgtgaagg taaaacactg atcctgtttc cggaagggac
    12241 gcgcggtgag ccaggcgtac ttgcgccatt gaaatccgga gtgtggcatt tgagtcagcg
    12301 catgccggag gtgcccatta tccccgttgg gttgcgcggt actgagcatg tgatggcgaa
    12361 aggtaatcgt atccctttgc cgctgtttat tgatgtaaat attggcgatg cgctaagtac
    12421 gcatccggag aagaaaacgt ttatggacga cctggagcaa agactgacgg cgcttttgca
    12481 accctcgcaa ggaaatcatc cccatgacta attcccgcat acccgatgaa cggcagtttc
    12541 tgaccagtga taatacagag ctgttttatc gccactggcc agcacaagaa gccagttcaa
    12601 cgccaaaagt ggtggtcctg tttcatcgtg ggcatgagca ctccgggcgt ttgcaacaca
    12661 ttgttgatga gctggcgatg ccggatacag cgttttatgc catggatgcc cgaggtcatg
    12721 gtcgctcacc gggtctgcgt ggttacagtc cgtcgctcgt gcgttcggtg caggatgttg
    12781 acgagtttgt gcgtttcgcc gccgcagata gccaagctgg gatgacggat gtggtcgtcg
    12841 tggcgcaaag cgtcggggcg gttctggcgg caacctgggc gcatgattat gccccggaca
    12901 ttcgtggcct ggtgttggca tcaccggcgt ttaaggtcaa attgtatgtg ccgtttgctc
    12961 gccaggggct ggaattgatg tatcgcctgc gcggtctttt cttcgtcaac tcgtatgtga
    13021 aaggcaagta tctgacgcac gaccccgagc gggtagcgag cttcaataag gatccgctga
    13081 ttacccgacc aattgctgtg aatattctgc ttgatctcta caaaacggct gagcgagtcg
    13141 tcagcgatgc cagcgccatt acgctacctg tgcagttgct ggtctccggg gaagattacg
    13201 ttgtgcaccg ccagccgcag cttgattttt atcagaacct gcgaaatccg cagaaagaac
    13261 tgcatgtact gccgggattt tatcatgaca ccttaggcga aaaagagcgg catctggcat
    13321 ttgacaggat gcgcagtttt attgatacct tgtatgcccg tcccgtgcag cgttttgatt
    13381 acagccagga agatcgctgg agccccggcg ccgatacctg gcgcatactg agcggtggcc
    13441 ctgcgctgta ttcgctggac gatatctctt accgctgttt gcgctacggg atgaaagtgc
    13501 tgggcacgca gtccacgggg gtgcgtctgg gatttgagac tggtttcgat tccggcagta
    13561 ccctagacta catctatcgc aaccagcctc agggcagcac ctcgttgggg cgcctcattg
    13621 ataaaaacta cctgaatagc gtgggctgga aggggatccg ccagcgtaaa attcatctgc
    13681 agatgttaat tcagcaggct gttgctcggt taacagagca gggtagcgcg gtccggatcg
    13741 tggatattgc cgccgggcat ggacgctatg tgcttgatgc cctgcaaggc gaaacggcgg
    13801 taagcgagat ccttctgcgc gattacagcg aattaaacgt ggcgaaaggt caggagatga
    13861 tagcctctcg cggtatggcg ggccgtgcgc gatttgagca gggggatgcc tttaatcgta
    13921 atgaactggc ggcactggaa ccgcgtccga cgctggggat cgtgtccggt ctgtatgaac
    13981 tgttcccgga aaacgggccg gtacgagatt cgcttgccgg gctggctgca gcgattgaac
    14041 cgggaggcat tctgatctac acctgtcagc cctggcaccc tcagttaaaa accatcgcct
    14101 ggtcactcac cagtcacaaa aatggcaaag cctgggtaat gcgcgtacgt actcagggcg
    14161 agatggacgc gctggtccgt gacgccggtt ttgagaaatg cacgcaacta attgatgaaa
    14221 tgggcatctt cacggtctct atggcagtac gacgtcaacc atgacaacgc ggcgtgcggt
    14281 gattattcag ggaatcggct ggctaatggt gctggccccg ttcttctttc ttacttatgg
    14341 catgacgaat cagtttaccg ccgggagaga aaacatcagc agcctggtgt ttgactggga
    14401 gcaccggatc ccgttaatac cgctgacgat tattccgtac tggaggtaag cgtcaacgga
    14461 gcaccgtatt gacgcttatt tattggtgag tactacgttc catggcagga gttcatcaac
    14521 tcggttggaa ggccattccg gcagtacgct caggatatgg cgcagatacg cttccggatc
    14581 gataccgttc agacggcagg tgccgatcag cccgtacaac agtgctccac gctcgccgcc
    14641 gtgatcgctg ccaaagaaca taaagttttt ctttccgaga cagactgcac gaagcgctct
    14701 ttccgcagca ttattatccg cctctgccag accgtcatca ctgtaataac agagggcgtc
    14761 ccactgattc agtacatagc tgaacgcttc gcccagtctg gattttttcg acagcgtgcc
    14821 attcttctcc accatccatt catgcagcga cgtcagtaac gatttgcttc gctgctgcct
    14881 gcaagacgct ctgactctgg taatccccgt atttcatcct cgatggcgta cagttcactg
    14941 attcgcttca gggcttcttc tgccgtcgca cttttgctgc tgatgtatac atcgtgaatt
    15001 tttctccggg catgggccca gcacgcaact tctgtcagcg caccaccttc acgttctgca
    15061 ctgaacagcc tgtcgtaacc tgtgaacgca tccgcctgca ggataccccg gaaggggcgg
    15121 aggtgctgct ccgggtgttt cccctgccgg ttcggtgagt acgcgaacca gaccgccgga
    15181 ggagatgacg aacccgcatt gcgatcatcc cggacatacg tccagatgcg ccctgttttc
    15241 gtctttttct ggcccggtgc cagtaccttt accggtgtgt cgtcagtgtg aagcttgcgg
    15301 gtattcatca cataacggta cagggcatca ttcagcggcg tcattaactg gcagcacgcg
    15361 tcaacccagt tggagagtaa tgcacggctc agttcgacac cctgtcaggc aaagatttca
    15421 ctctgacgat acagtagcag gtgttcgcag tattttcccg ttaacacgcg ggcaagtaat
    15481 ccggggcccg caataccacg ctctatcggg cgggacggtg ccggtgcttc aacgatgcag
    15541 tcacattttg tacaggcttt ttttacccgt tctgtgcgga tcactttcag ggcgctgctc
    15601 accagttcca gttgttctgc actgacctcc cccagataat ccagctcacc gccacactcc
    15661 gggcaacagc tttcttcaga ctccagacgg tgtatttcac ggggaaggtg tgccggtaac
    15721 ggacgacgat ggcacgactg tcgcaactgg cgcagttttt ctatctgagc ccgcagatgt
    15781 tctgcttccc gttcatcttc ttcgatcttt tcttcggcac gtgtcagtgc agagcgcagg
    15841 aaggcttccg tctcttcaac cagactcagt tgctggtctt tctgacggag ggcttcagcc
    15901 tgctcagaga gcaacctttc cagctctgcg atgcgaatga ggtatttctg actcatgacc
    15961 gtttttataa tgcggtcagg agttttttac aacattgtca gtgagttacg gcgggatgtt
    16021 tttggctgac gccagtccag cttatcgagg agcattgcca gttgcgagcg ggtaatggat
    16081 accttgccgt cacgtaccgc aggccagata aactggcctt cctccaggcg tttggtgaac
    16141 aggcacagac catcagcatc agcccaaaga attttaacgg tgtcaccccg tcggccacgg
    16201 aagataaaca ggtgaccgga gaagggatta tcattcagca catgttgtac ctgttctccc
    16261 agtccgttga aggatttacg catatcggta acgccggcaa cgagccagat acgggtacct
    16321 gatgggagtg agatcatctt cccctcccgg tcagttcacg gatcaacacc gtgagcagct
    16381 ctggcgatgg attttccagc gtcatgttac cgtgacggaa ttccaccttg caggagctgg
    16441 cactgactct ggtctgagtg gtagtggata aagatggcgc aatggccact acaggttctt
    16501 tctgctcatc cggcgttatt tctacaggta ataattcaac gccagtgtca gaagaggtcg
    16561 ttaccggaag acgccgcgaa acacgccctt cgttctgcca gagcctgagc catttgaaca
    16621 ggaggttatc attgatatcg tgttcccggg cgatacgggc aacagaggct cctggttgca
    16681 aagccagttt aaccatttga agtttaaaat tatttgaaaa tgatctgcgg ggttctgcgg
    16741 ataatgtttt ttgctccata acaggcgtcc acaggatggc atgcggaata tcaacatttt
    16801 actgataatc ttccgttaca ccttcgtgag cggattatgg tatgtgctca cccggatgac
    16861 attgatgatt tttctgataa ggttatccaa aaagccatga tgttgaggac ataaaatgcg
    16921 ccagagagta atagtgtgcc ctgtgataca aaatgaaggt gagtttctac tttgtaaaat
    16981 ggcctctgat cgtggagtat tcccgggcca atgggcactg cccggtggag gaatggaacc
    17041 cggagagact atggaaacag cattgaggcg tgaaataaag gaagaactgg gaaaagaact
    17101 gcatatcact gacgtaaaac cctgggcctt tcgtgatgat atccgtacaa aaacgtaccc
    17161 ggatggtacc cacgaggaca tttacatgat ttacctcata ttcgactgta tcagtacaaa
    17221 ccgtacagtt acatttaatg aggagtttca gggtatagtc tgggcatctc ctgacaccct
    17281 aaaatatatg gatttaaatg aagcaacccg gatcacattt tctcagaaag gtatccttta
    17341 aactccaatg ctaatcgtgc aactgataac ggcttccaca acagggagcc gttttcttat
    17401 gccactactc aaggatctgc tcgatttcag tgaccatccg cttatgcctc cacactctgc
    17461 acaactgttt gctgaacacc ttcccgtcga gtggatacag cactgcctga cgcgtcctgc
    17521 tcatgagacc gttcggttcg ccgtcgtcgt ttaccggggg acatgggatc cgcttggaat
    17581 tcggaaattt ccccacgcta agagaattga gttgaacaag tgcttacatc ccggcaagct
    17641 aactcactga atgtcaaggc tatacgaaat gttctgaccg tctgataacg accgggcccc
    17701 gtgctgacac gggacattga acgggtgcta gagcgaagcg gggaagctcc ggtctacgga
    17761 ccaaaatgaa aaacagggct tctggagaaa atggttccac taaatagctg cgccgaatag
    17821 tagatcactt tgagggaact cagcccggat tgcgcgatct gatcaattgc caaaccaatc
    17881 aaaatcacca accggactga gcgatgccga tcatagcacc aattccccgt gacgaactgt
    17941 cacgaacggt gcaatagtga tccacaccca acgcctgaaa tcagacctag gggtaatctg
    18001 ctctcctgat tcaggagagt ttatggtcac ttttgaaaca gttatggaaa ttaaaatcct
    18061 gcacaagcag ggaatgagta gccgggcgat tgccagagaa ctggggatct cccgcaatac
    18121 ggttaaacgt tatttgcagg caaaatctga gccgccagaa ccacggcagc ctggcggctg
    18181 caaatcgggg tgtggcagaa tatgagcttt cagagtaacc atccagaatg agttcccatg
    18241 tcacacaacc tggagcacca gaaagtccac accagaatgg taaaagaggt tctgaaagcc
    18301 gtcgccagag caaataatca cccttatcag tccgtatttg cagactttat cgccggtcat
    18361 tcctcctgca cggtctgttt ctgggaaacc ttccacaaaa tgtacccgga ttcaccgtac
    18421 gaatacgtca cgttctgcca tacctgccgt cgttttgatt tatacgaaac agaagcggag
    18481 atgaaggcgg atgacccgaa gtggtggtga ttttcagccc cgaccgctga aacgcctgtt
    18541 cacagccaac cagtgctgga cgtccttcct ggatgcgggc ggtctgcgcg atatcgaagt
    18601 ggaagccgtc accaaaatgc tggcctgcga cacgcggata ccgggcgtga aggaatacaa
    18661 ctgcgataaa cctgactgcc cccacgtcag atacgtcact aactcatgcg gctgccgtgc
    18721 ctgcccgtcc tgcggaaaga aggccacaga cctgtggata gcgacacagc tgaatcgtct
    18781 tcctgactgc gactgggtac acctggtctt caccctgccg gacacgctgt gccggtgttc
    18841 gaaagcaacc gttggctgct gaatgacgtg tgccgtctgg cggtggagaa tctgctgtat
    18901 gccgcccgaa aacgggggct ggaaaccggt atcttctgcg ccatccacac gtatggccgt
    18961 cgtctcaact ggcatccgca tgtacatatg tctgtaacct gtggaggtct gaataagcat
    19021 ggtcagtgga aaaagctgag cttcctgaaa gacgcgatgc gttcgcggtg aatgcggaat
    19081 atgcggcagc tgcttctgaa agcgtggtct gaggggctgg cgatgccgga gtcgttgtca
    19141 catatcacga cggaatcaca gtggagaagc ctagtgctga aagccggagg aaaatactgg
    19201 catgtgtaca tgtcgaagaa aacggccggc gggaggaata cggcgcgcta cctgggtcgt
    19261 tatctgaaga agccgccgat agcagcctcc cggctggcac attacaacgg aggggcgagc
    19321 ctgagcttcc gttacctgga ccacaaaacg ggagaaacgg cgacggaaac gctgacacaa
    19381 catgagctgg tcgcgaggct gaaacagcat atcccggaga agttttttaa gatggtgagg
    19441 tacttcgggt tccttgccaa ccgggtgtgt ggagagaagc tgccgcaggt gtaccgtgca
    19501 ctggggatgg ataaaccgga agcagtggcg aaagtgtgct atgaacaaat ggtgaaacag
    19561 ttcctgagtc gtgacccgtt cgaatgcgtg ctgtgtgcgg ccggatggta taccgccagg
    19621 cccttgcggg gctgaatgta tcagggctga agaaaaacgc gcgggatatc agtctgctga
    19681 ggtatatgcc ggcctgacgc aggctaagtg tgcccgggaa ttggtgcagg aataaaaaac
    19741 agcctgtcgc ggtataaaaa ccgcccatac acagagagaa aacaccggag tggagggaaa
    19801 agcagaagag ctcacgtggc ataaggcgtg gacagcggta tcgtctgatt tttcaatttt
    19861 gtttttcaat ttcctatacg tgaacttcac ttttccttag cgtgtattat cgcagaaatg
    19921 cccacggaac ccttacaata ttgtacgttt cccaaacgca gcccatcagg cgcggaactc
    19981 atcgaggcgc gggaataaaa tcccattcag acgcaggata gattcaagca agctcactta
    20041 tcgtcaaaat cggtgttgca aaaacgggag tgaccataga ttcgtttccc aaaccgcccc
    20101 cttttatatt gaccagaagt gttgtacagt ttaattgtac aactcaaaga ggaagcacta
    20161 tgtttacatg taactatagc catgctcgcg ataacctggc ctcgttgctc gagctggcgg
    20221 ttgatgaaaa tcagcctgtt gagattcagc gccgtaaagg tggcgcggct gtactagttg
    20281 ataaagacgc ttttgaacga taccgtaaag cggctcttga tgctgagttt gctgagatca
    20341 tgcaaagtca tgggacggcc tatacggagc tttctaagcg atgaaattcg ttagttcaca
    20401 ggaagtgatt gagtttcatg accgcttgat ttcgcgtgat ggtggcgtgg cgggtatgac
    20461 agaacccggt cgcgctgacg cgatcattca tcgtgttttg aacatgtatc attatgaagg
    20521 tgtgacagat attatggatc tggctgccgt atatttagtt gctattgctc gtggtcacat
    20581 tttcaacgat gcaaataagc gaacagcctt gtttgttgca caggtttttt taaaacggaa
    20641 tggtgtacat attattagtt cacgtatttc gtttgatgaa atgcaaatta tcgctttgaa
    20701 tgctgcaaca ggcgaatata actggaagat ggtttctgac catcttaaag ccattattct
    20761 aaattagata tagatatcca atcagcagca ttttacaatc tcctgagcaa tgtaaaaatc
    20821 atatatcttt ttactatata atttctggcg tatgtgctca attcagacct tacacccttc
    20881 cgcttcctcg cccactcgct cactaccgtt cggtcgctgg ggctgcggcg agcgtattgt
    20941 aagtttttca atacagacga cgttctccct ggctgattaa tattgtgtaa taatacatat
    21001 cgttttcagg aataatgcgg tcaattactc tgatgggcaa ggccagatta cctggcagct
    21061 tcggaaattc cccctagtgc ctcgattacc tggtgtaacg caacaaaaaa aaccgcctaa
    21121 accaccaggc tcatttattt cttccagggc attagcaaga taagcgtttg cataatcagg
    21181 atctgcgcgc aattcttcaa tcatggcgac gttgtgatca acggttgtcg aatagcatga
    21241 aagattcaag tgagcctcca gagaactaat tcagggcttt caaaaaagaa tccttgcttg
    21301 attatcagga tttcagcgca tatctgcgat ctgataaact tcgttacctt ccctttttcc
    21361 aacggaaata acgagtaaaa caactttttc atcaatgact tgatatacaa gtctgtaacc
    21421 agatgtacga agtttgattt tgtaacggtc agcatgacca tgtaacctgg atgcaggcac
    21481 gcgtggattt tccaggcgtt cagacagttt ctttttgaat tgttcgcgga tggtgtggcc
    21541 cagcttctgc cattccttca gtgccctgcg gtcaaaagcc agttcatagg tcatccaggt
    21601 taaccttcac aatttccgga tttttcatgc gctcatcagc aacagcgttt aattcggcat
    21661 cttcagcgag ttcccgataa taggcgtaaa gttttggggg gacgcagtag aacgctggtt
    21721 cgtttcggtt caggatcaca actgcgtctc cctcgccttc agctacggtt cccataggat
    21781 tttttttcag gtcagtaata ctggctgctg tagttgtcag aatttgatag ctcataagcc
    21841 ctccgtttgt ctcgttgaga taatagcatc tttaaaaaca ctcttaaaag acctttattt
    21901 tgaagggggg atgaaactga tggaataggg ggtccaggcc gcaaccgccg aaatttccgg
    21961 gatgcacaaa ttttatgttc agcaacctgt acgtcgaatt atctccttaa cgaacgtttt
    22021 cgtacgg
//
LOCUS       DAZGAN020000075        21942 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGAN020000075 DAZGAN020000000
VERSION     DAZGAN020000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.075"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            282..524
                     /locus_tag="ACHKYO_RS15870"
                     /old_locus_tag="pgaptmp_003174"
     CDS             282..524
                     /locus_tag="ACHKYO_RS15870"
                     /old_locus_tag="pgaptmp_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429977.1"
                     /translation="MSNEYQSTSINPDVFEQFFTLLNTHCKSSEAALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
     gene            complement(617..2152)
                     /locus_tag="ACHKYO_RS15875"
                     /old_locus_tag="pgaptmp_003175"
     CDS             complement(617..2152)
                     /locus_tag="ACHKYO_RS15875"
                     /old_locus_tag="pgaptmp_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4429978.1"
                     /translation="MSCIKEKSKYAFERAKQFYFMLWCKESLIYLLFSVLVTWCCGYT
                     VKNTLGNVLILFLMLLLGRQNVISATCVCIFLLLSAFYAPVGMIYGKINNSFIASVMQ
                     TNLNEAGEFITGIPVLYFIVSLIIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPK
                     RMVTNVMTGIAETREEMMRYESLKHNQKDSWDIVNVEKKYKTIIIVIGESVRRDYLSV
                     YGYPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKA
                     GYETWWISNQGSLGRHDTLISVIAANADKKYFLKEGSSSSRRIDDEDMLQYVREAVNS
                     SSPKVIFIHMLGSHPNPCDRLFNDAQPFRELFGNKVSCYLSTLLKLDRFLQAIYNMTT
                     EHGDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISA
                     RRFPEIFQWITGIQTKNLPLSFSLLQNDFAESVSVFNGERDVPVMSLPEEPILTGNAE
                     SEK"
     gene            complement(2352..2807)
                     /locus_tag="ACHKYO_RS15880"
                     /old_locus_tag="pgaptmp_003176"
     CDS             complement(2352..2807)
                     /locus_tag="ACHKYO_RS15880"
                     /old_locus_tag="pgaptmp_003176"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HGU4429979.1"
                     /translation="MRDKSEVLTGAQKNRFGLILYIILLSVVYILITRLNLTTTYYSL
                     YSSLDENSQVTEYFIISIATFLFLAALCLFFIYSFFKRKRVVGKLLLCIYILYISHYA
                     ITFVYCLYVVGAECMTTGKIEHILIDIIIAMLSLSYVCLSGRARHIFVN"
     gene            complement(2895..3518)
                     /locus_tag="ACHKYO_RS15885"
                     /old_locus_tag="pgaptmp_003177"
     CDS             complement(2895..3518)
                     /locus_tag="ACHKYO_RS15885"
                     /old_locus_tag="pgaptmp_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4429980.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRETGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKAPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDSLTGTTRLVSAYTL"
     gene            3667..4040
                     /locus_tag="ACHKYO_RS15890"
                     /old_locus_tag="pgaptmp_003178"
                     /pseudo
     CDS             3667..4040
                     /locus_tag="ACHKYO_RS15890"
                     /old_locus_tag="pgaptmp_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            4684..5049
                     /locus_tag="ACHKYO_RS15895"
                     /old_locus_tag="pgaptmp_003179"
     CDS             4684..5049
                     /locus_tag="ACHKYO_RS15895"
                     /old_locus_tag="pgaptmp_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429981.1"
                     /translation="MNDGRQTPPSPEAWKIRFLADGTPGTWFYTDDAAVTKEWLRAHC
                     DDVEVIPLCRMHDGPVFREHHINELSAALARMLHMHHILLERTRYRENIHDAECIREI
                     HEAPLQAIGILEKYKRTKS"
     gene            5059..5250
                     /locus_tag="ACHKYO_RS15900"
                     /old_locus_tag="pgaptmp_003180"
     CDS             5059..5250
                     /locus_tag="ACHKYO_RS15900"
                     /old_locus_tag="pgaptmp_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429982.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFTAL
                     KKENVGEFALRGVCVPGGE"
     gene            5284..5607
                     /locus_tag="ACHKYO_RS15905"
                     /old_locus_tag="pgaptmp_003181"
     CDS             5284..5607
                     /locus_tag="ACHKYO_RS15905"
                     /old_locus_tag="pgaptmp_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HGU4429983.1"
                     /translation="MAYHSPDITLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRMREQMSRIIRKTGTDEILFRLSKNHHLYEKK
                     KTKEG"
     gene            complement(5753..5848)
                     /gene="dqlB"
                     /locus_tag="ACHKYO_RS15910"
                     /old_locus_tag="pgaptmp_003182"
     CDS             complement(5753..5848)
                     /gene="dqlB"
                     /locus_tag="ACHKYO_RS15910"
                     /old_locus_tag="pgaptmp_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HGU4429984.1"
                     /translation="MINLLIIVLRAVVAVVNAVIALLELIRELID"
     gene            complement(6499..6594)
                     /gene="dqlB"
                     /locus_tag="ACHKYO_RS15915"
                     /old_locus_tag="pgaptmp_003183"
     CDS             complement(6499..6594)
                     /gene="dqlB"
                     /locus_tag="ACHKYO_RS15915"
                     /old_locus_tag="pgaptmp_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HGU4429985.1"
                     /translation="MINLLIIVLRAVVAVVNAVIAILELIRELID"
     gene            7139..8464
                     /gene="trbA"
                     /locus_tag="ACHKYO_RS15920"
                     /old_locus_tag="pgaptmp_003184"
     CDS             7139..8464
                     /gene="trbA"
                     /locus_tag="ACHKYO_RS15920"
                     /old_locus_tag="pgaptmp_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HGU4429986.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFSQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEAFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQKWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEACRLGLPCPEPCVEEAIEGLRQDMLRLGLIWDEPQPDRDRRRQIRTRWSLTDDVI
                     PRRHDNDEGSDTGETTETTETTETTETTETTETTETTETRHPADKEKAQ"
     gene            8461..9579
                     /locus_tag="ACHKYO_RS15925"
                     /old_locus_tag="pgaptmp_003185"
     CDS             8461..9579
                     /locus_tag="ACHKYO_RS15925"
                     /old_locus_tag="pgaptmp_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HGU4429987.1"
                     /translation="MTIRYTATLNNKHLSLTALYPDGGQEVIAILPVTELGALINRNN
                     RLMHLSFSTPFREITETLLVDTVDTERPGHHIGRMLSAIDSCLSAAYSELPARKTSFM
                     RGLIYGAGLVLALLTAVTAGYILLKPDSDSPLRVPVTESVTETAPLHIDKEIVRSVAP
                     SETKNEIVHPAPQEMQETSATPAITADKNTLVAEARRNLATVLKRNADRGMFTINLST
                     GHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGEASTNQTAPLLCEPD
                     AQKRATGWHQLYSADGGMAPTGDAPAAPDEKCLTAARAAIDVNNQAFRQFGFGGTPWV
                     LSDTGWHLPSGILKDATTVSLFLKATDTEDNHNEPASR"
     gene            9560..11863
                     /gene="trbC"
                     /locus_tag="ACHKYO_RS15930"
                     /old_locus_tag="pgaptmp_003186"
     CDS             9560..11863
                     /gene="trbC"
                     /locus_tag="ACHKYO_RS15930"
                     /old_locus_tag="pgaptmp_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HGU4429988.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFTLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHIIFFSTTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFITQQSMILARDLGYRLEGTDAQTLEVKKYKGRFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKINVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTEKTSRKRRKKHTEPYRIIDTFQDQLKRTQTLLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            12330..12572
                     /locus_tag="ACHKYO_RS15935"
                     /old_locus_tag="pgaptmp_003187"
     CDS             12330..12572
                     /locus_tag="ACHKYO_RS15935"
                     /old_locus_tag="pgaptmp_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429989.1"
                     /translation="MKTNMGSTKNGARVPHVRNRFTSMDIGMNSQILPDMLINFALIN
                     ITDRKNEGTNTIDGNWQADEGRRYRDNVRIYFSQRP"
     gene            13285..15333
                     /gene="ireA"
                     /locus_tag="ACHKYO_RS15940"
                     /old_locus_tag="pgaptmp_003188"
     CDS             13285..15333
                     /gene="ireA"
                     /locus_tag="ACHKYO_RS15940"
                     /old_locus_tag="pgaptmp_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HGU4429990.1"
                     /translation="MKNKYIIAPGIAVMCSAVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQVHTLTPGESLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYNNDRGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVNDKWRVSANYTFTDSRRESDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGLNYQILPDMLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRVSF
                     "
     gene            complement(15579..16010)
                     /locus_tag="ACHKYO_RS15945"
                     /old_locus_tag="pgaptmp_003189"
     CDS             complement(15579..16010)
                     /locus_tag="ACHKYO_RS15945"
                     /old_locus_tag="pgaptmp_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HGU4429991.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(16078..18789)
                     /locus_tag="ACHKYO_RS15950"
                     /old_locus_tag="pgaptmp_003190"
     CDS             complement(16078..18789)
                     /locus_tag="ACHKYO_RS15950"
                     /old_locus_tag="pgaptmp_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HGU4429992.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(18801..19133)
                     /gene="mobA"
                     /locus_tag="ACHKYO_RS15955"
                     /old_locus_tag="pgaptmp_003191"
     CDS             complement(18801..19133)
                     /gene="mobA"
                     /locus_tag="ACHKYO_RS15955"
                     /old_locus_tag="pgaptmp_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HGU4429993.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            19366..19701
                     /locus_tag="ACHKYO_RS15960"
                     /old_locus_tag="pgaptmp_003192"
     CDS             19366..19701
                     /locus_tag="ACHKYO_RS15960"
                     /old_locus_tag="pgaptmp_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HGU4429994.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKTSRRSSSKTAPKKTDSAR
                     KDGIDMNNN"
     gene            complement(19786..20634)
                     /locus_tag="ACHKYO_RS15965"
                     /old_locus_tag="pgaptmp_003193"
     CDS             complement(19786..20634)
                     /locus_tag="ACHKYO_RS15965"
                     /old_locus_tag="pgaptmp_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4429995.1"
                     /translation="MNQTLPTADLNTDGTTDVIPSVAIDRIIAQRNEGISLFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEIILDNVLATFRHLNASKMQTFEQGLIDVWRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            20983..21179
                     /locus_tag="ACHKYO_RS15970"
                     /old_locus_tag="pgaptmp_003194"
                     /pseudo
     CDS             20983..21179
                     /locus_tag="ACHKYO_RS15970"
                     /old_locus_tag="pgaptmp_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21213..21464
                     /locus_tag="ACHKYO_RS15975"
                     /old_locus_tag="pgaptmp_003195"
     CDS             21213..21464
                     /locus_tag="ACHKYO_RS15975"
                     /old_locus_tag="pgaptmp_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4429996.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(21495..21701)
                     /locus_tag="ACHKYO_RS15980"
                     /old_locus_tag="pgaptmp_003196"
     CDS             complement(21495..21701)
                     /locus_tag="ACHKYO_RS15980"
                     /old_locus_tag="pgaptmp_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4429997.1"
                     /translation="MTIAERLIQKGFDEGFKEGFKQGFKQGALEVAREIACRLRDIGW
                     PPERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(21763..>21942)
                     /locus_tag="ACHKYO_RS15985"
                     /old_locus_tag="pgaptmp_003197"
     CDS             complement(21763..>21942)
                     /locus_tag="ACHKYO_RS15985"
                     /old_locus_tag="pgaptmp_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4429998.1"
                     /translation="ERLPQHEDKLMTIAERLEQKGALEKALAIARQLQKMGMTPEQIK
                     QATGLSEAELKKVIH"
BASE COUNT         6007 a         5645 c         4877 g         5413 t
ORIGIN      
        1 taggcattac agtgggccct gggttgattg ataaaaaaat cgcctggggc tttctgctgc
       61 atgccgtcca caaaacgctt tcagcataca gcctcaagcg cccgccgcta ttctaatcga
      121 ttttcagtct gccacaatcg ttttccacct aatgacatgt aatgcacaac agtattttta
      181 cgggaaacta cacggcaagg attcccggaa cacgttcatt attttatggt tacagacatc
      241 atgttaaatc cggaagtaat aatgacctgg aaggaaatac tatgagtaac gaatatcaaa
      301 gcaccagtat aaacccggat gtttttgaac aattttttac cctcctgaat acacactgta
      361 aatcatcaga agctgcactt gccatgctga gtacactctg ctacgaactg atgaggagga
      421 gtgggatcca ttcactggac agcctgatgc tcaacggaga gggtatattg atacaattac
      481 agggggctga tattcgcgaa aaaacaggtc agcatattca ctgatccagg tatgggctga
      541 aaacagtgtc agcctgaaat aaacgctcaa tgcctcatac acatgaggca ttgagctcgg
      601 tcataaataa tctttattat ttttctgatt cagcatttcc ggtcagtatc ggctcttctg
      661 gcaaagacat taccggaacg tcacgttcac cattgaatac agaaacactt tcagcgaaat
      721 cattctgtaa caaagaaaaa cttaacggca gatttttggt ttgtatccct gtgatccatt
      781 gaaaaatctc gggaaaacga cgtgcactga tatgcacatg attgaggtgg tgctcagtaa
      841 tatcactggc catcatcatg agaggtacat cgtatcccga acgcaaacct ggcgcatgat
      901 gcacaggggt gctgtctgaa ctgacggatt gcccatgatc agaaaaatac agcatggcaa
      961 aatcatcacc atgctctgtc gtcatgttat aaattgcctg aagaaaccga tccagcttaa
     1021 gcaatgttga taaataacag gatactttat ttccgaataa ttccctgaac ggctgtgcat
     1081 cattaaaaag tctgtcacag ggattaggat gggaccccag catatgaatg aaaatcactt
     1141 ttggagaaga cgaattaaca gcttccctga catactgaag catatcttca tcatcaatgc
     1201 gacgggaaga agagctccct tccttaagaa aatatttctt atccgcattg gcggcgatga
     1261 cagaaatcag cgtatcatgt cgccctaaag agccctgatt tgatatccac cacgtctcat
     1321 atccggcttt tctggcaagc gtaacaatat tattacccgg attacctgtt tcagtgtaat
     1381 caagcgtcag tgttctgctg agtgaaccta tcgtattcgg agctgcggag aaatacccat
     1441 taataaatat acccggagca gaatttaacc atggtgttgt cggtaaagga tacccataaa
     1501 cagaaagata atcccgtctg acactttcac caattacgat aattatggtt ttatatttct
     1561 tctccacatt cacaatatcc catgagtctt tctgattgtg tttcagcgat tcatatcgca
     1621 tcatttcttc tctggtttca gctatccctg tcattacatt cgtaaccata cgttttggcc
     1681 atgcatttac tgacaatacg ataaagaaca acagaaagat aaatccccgg tgacttttat
     1741 cacgcttacg ccagaaccag aatgtaaaag cagcaatgat cagtgaaaca ataaaataaa
     1801 gaacaggtat gcctgtgata aattcacctg cctcatttag atttgtctgc attacagacg
     1861 caataaaact gttgtttatt tttccgtaaa tcatcccaac cggagcatag aatgcactta
     1921 acaaaagaaa gatacagaca cacgttgctg aaataacatt ctgccgcccc aacaacaaca
     1981 tcaggaacaa tataagcaca ttccccaggg tgtttttcac tgtatatccg caacaccatg
     2041 taacaagcac agaaaacaac agataaatca gtgactcttt acaccacaac ataaaataaa
     2101 actgttttgc tctctcaaaa gcatattttg atttttcttt aatacaagac acgccagtca
     2161 ttttcctgta caaacacaga cgatcatttc aacacatgga tatttatatg aaacagttca
     2221 caaaaatacc ctaccctgtc cagcagggtt tacatattat caggttctgt gtttacagca
     2281 actgctcccc gaaccagatt attcgcataa cccgatgaag gcctcataac cataaaacac
     2341 tcagaataca tttagttaac aaaaatatgt cttgccctcc cggaaaggca aacatacgaa
     2401 agagaaagca tggctataat aatatcaatc aaaatatgct caatctttcc ggttgtcata
     2461 cactcagcac cgacaacata aagacaataa acaaaagtga tagcatagtg tgatatataa
     2521 agaatataaa tgcacagaag taatttcccg acaactcttt tccgtttaaa gaaagaataa
     2581 atgaagaaca aacataaagc cgccagaaaa agaaaggtag ctatggatat tataaaatac
     2641 tccgtcacct gtgaattctc atccagagat gaatacagcg aataatatgt ggttgtgaga
     2701 ttaagtcttg ttatcaatat ataaacaact gataatagta taatataaag aatcagacca
     2761 aatctgtttt tctgcgctcc ggttaatact tctgatttat ctcgcataaa atatcctgtc
     2821 tgtaatctgt cccggtaaac aatggtaaat ttcagaatca gtgcgaatac ggtaacaaag
     2881 attcctcctg tgctttaaag tgtgtaggca gaaaccagtc tggtggttcc ggtcagggag
     2941 tctgatacct caacttcccg ggtgactata accatacctg actgacgcag tctctgcaca
     3001 tcagctgcaa ttcgctgagt tcccagccag aacaggccat ccagtgccgt taccagcaac
     3061 ccgctttcca gcgccagttt cagccgtgcc gtcggggctt taacctgccc ggcgatatca
     3121 gtaaaacaac gcgtcgtcat gccatagcag tccatccgcc gtattcgcgt cgcaagactg
     3181 tagctgaaac aatccggcac gccggataag tcattctgca gactgtatac acatccccgc
     3241 tgccgcccgt tgaacagggc ttttttctga ctgagcagcc attcccgacg cagagtgtca
     3301 atcatctgcg gtgtccgggt taacagcaga cgaaacggca gctcaaaact ggtcacataa
     3361 tcaacattca gtaatgctgt ccgcagtcgc tccctggcac ctgccccggt ctcccggcat
     3421 tcacgcatga catcagccca ctgacgcatc agtcgtggag actcattcct ggccctgaac
     3481 tgatatttct caatatgata atcaatacgt tcctgcatat cgccaccgga tatctgttgt
     3541 ttacccagac acagtgatta gcctacagga ataaatatcc tccgtgaagc caggtgcctc
     3601 agaagataaa aacatgtact tttttcaacg tgtaatatta acaccaccac cacaggaaaa
     3661 tataaaatga taaaatatat attcgcactc acacttctgc tttccgggtc tgtctgtgcc
     3721 gatcaaattc agggactcgt tattcgtgtg ctggacggag atttgtggta aatacgcccg
     3781 taaccgtgac atacacacag acagaccgct acgggcgtat tcttggacgt gtatataccg
     3841 aagacggcat ggaagttaac cgctggctgg tacagcatgg tgcagcctgg gtatatcctg
     3901 atttcaacac agattacacc ctgccggtat accagagaga agccagaaca atgaaacggg
     3961 gtctgtgggc agacaaaaaa cctgttccac catggctgtg gcgaaaagaa aataaaaata
     4021 gccacaataa ttataaataa tacatctcac agaaatatat ttcctgccgt tttatttccc
     4081 ctgccagtcc ccaacgggaa ataacctcca gaaattacac agatgtgaat aataaacctg
     4141 ttcgatgtat ccgtcacaaa agagttgatg tttgactcag aagaaaatac gcctgaagtt
     4201 aaggaaggct tcagcttcat gattgttctg acatgaacat atatccctct gtagcgccct
     4261 gcctttcata acatttctgt tacagcaaca acgaaagaca acagcaatgt cggcctttta
     4321 cgtccacatg aatattccac ttatccggaa ttgtgacaca gcccctgaat acaggattga
     4381 ttgccagtgc ccacaaatgg cacattttca gtaagattct gaatgttaca ttcccccgtc
     4441 atgtgtggca caactgtcac cgggaaagaa acggaaagca catgaaaaac atcaaaactc
     4501 acaccggact ccttatccac aaagaacaag cgcggcgcgt tcgtcttcat gaaactccgg
     4561 ccgcctggtg ccacacgcac cgggaatgct acagcaaaac gaccggtcgt cgctgcggca
     4621 gccctgacag tctcagcagg ctgattctga gcagcattca gcccatagaa tgagatgacc
     4681 cgtatgaatg atggcagaca gacgcctccg tcccctgaag catggaaaat ccggtttctg
     4741 gcggacggta cccccggcac atggttttac accgatgacg cagccgtcac aaaagagtgg
     4801 ctgcgggcac attgtgacga tgtggaagtg atccccctgt gccgtatgca tgacggtccc
     4861 gttttccggg aacaccatat taatgaactc agcgccgcac tggccagaat gctccatatg
     4921 caccacatac tgctggaacg aacccggtac agagaaaata tccatgatgc agaatgtatt
     4981 cgtgaaattc acgaagctcc cctgcaggct atcggcatcc tggaaaaata caaacgcaca
     5041 aaatcataag gcagttctat gtggatcctg attttctggc tctcatcccc ctttaatgtc
     5101 tcctccggcg atgcccggca acccacactg gccgttcaca gtcaggagtt ccgtactgaa
     5161 aaagcctgtc gggatgcctt caccgcgctg aaaaaagaaa acgtgggaga attcgctctc
     5221 cggggcgtct gcgtccccgg aggagaataa accggagatg atgattagcc acaggagtat
     5281 tcaatggcat accattcccc ggatatcaca ctgcattatc cgtatctgcg ccggatgtcc
     5341 tgggcgcaga taacggcagc agctgaagat gcggaaatac atcatgatta tgcccgggca
     5401 ttaatattgt ggcagcatgc atatcatgct gccacgctga ccctcaacaa aaacctggca
     5461 gttgctaaaa tcgacttctg tattaaacgt atccggatgc gtgaacaaat gagcaggata
     5521 atccggaaga ctggcacaga cgaaatactc ttccgtctga gtaaaaacca tcatttatat
     5581 gagaaaaaga aaacaaaaga gggatgacag tcccattgta tgggaaaaac ttgccatgct
     5641 tactgcaggt gcatttttga tactggcagt cctgggtgag taatcttaat atcctgttta
     5701 ttaaagggca gaaggcggtc cccctctgcc cttaagtacg tttccgggat tatcagtcaa
     5761 taagttcccg gatcagttcc aggagagcta tcactgcatt gaccacagca acaaccgccc
     5821 tgagaacgat gattaacagg ttaatcatcc gtttctccgt caatggagga agtcaccgtt
     5881 gcttgctcat acatcgcctg tcacaacggc tctctgacat tgttggatgt agagtacctt
     5941 cccccctgcc agagcactga acacagcacc acctgtttgt tcagccagga ctttaattca
     6001 tccatacaca gatagaacag gttttagaag caagggaggc actctgtaca gcgccaacac
     6061 aaatattata cagatgcaca cataaaattc atttaaagaa atgaatattg acaggcaatc
     6121 tgaagtcaaa ctagacaaca gatgtctggt gtcataatat tatggatgtt ggatgtagag
     6181 tatcttcctc cggtcagagc accaactccg ctccggaatc acaaaaaacc ggcctcacca
     6241 gtcggttttt ttacgtctgt acctcccctc ctccccggac aactgctctc tcactcccgt
     6301 tgaatatttc ctgttgattt ttacgaaaat acggcaaatg ggggatatcg aatatatttc
     6361 ctggtgtgta gtcggtaaaa accagctcat gtttgactca cagaaacagg ctctggaagt
     6421 acagaaagta atctgtccgt agtttattaa agggcagagg gcggttaccc actgccctta
     6481 agtacgtttc cgggattatc agtcaataag ctcccggatc agttccagga ttgctatcac
     6541 tgcattgacc acagcaacaa ctgccctgag aacgatgatt aacaggttaa tcatccgttt
     6601 ctccgtcaat ggaggaaatc actgctgcgt actcttacat tgcctgtcac aacagctctc
     6661 tcattgttag ctgaaaagag acttcctccg gccagagcac cagacacagc accacctgtt
     6721 tgtccggcca ggtctttgaa tagcacagat ccatagtccc gatggattga tgcgaaggta
     6781 agtctccaga caaggctaac ttactgatta tacttgtttc aggcgatatc gctactcccg
     6841 gaataataga ttgtaactga cagattgcga tcaggtgaat ataacgctat tctggtaacg
     6901 ttagttgaaa agagacttcc tccggtcaga gccaccaact ccaccccgga atcgcaaaaa
     6961 aaccggcctc accagtcggt ttttttgtac ctgtctccca ccaccttctg ccgttcgctc
     7021 ttctctgtga aaatgtcctg ttgaactcat tcctgtccgg ttattaccgg cgctgattcg
     7081 cattcatgcc acccgactta atcccgaaat ttatccattc atctatggag gaatccccgt
     7141 gtcttaccaa cagacgtctg ccgccgaaga tccaatggct atatggtata tcgtcggtgc
     7201 catatgcctg ctctttgcga ttatcatctg gcgcttttta cctgaaatcg tcttcgcctc
     7261 ctgcctgatc ctgcacacac tgtggggcat gattgactgg gggccctttc acaactttgc
     7321 cgccccccgt tacaacctgc tggccatcac tgctaacaac gcggcaacca tcactttcag
     7381 ccagtggctg gatgttatgt cccggacagt ggggatcctc tggttgattc tgcttcccat
     7441 gacattcggc tttctgtgga tgtggtttca ccatccggcc cagccccgtt ttacccgcag
     7501 gccactgaat atccacacac tgccccatat tttttcagct ctgtcaccgg cgatcgcccc
     7561 cgttctggct gacggagaca acaacagact cttccacgga caaaaacgtc ccgagcgccg
     7621 tgtcgcacta acccccgaag cctttgtgga gcagaacaac cttatccgca acatgcagct
     7681 cgatgttgcg tccacacgcc agtgcttcat ggcgcaactg gggcaaccac tgaccagctg
     7741 gaaagacatg gccccacatg aaaaagcgct ctttgccatc tttggcctgc agttcttcct
     7801 cggtgaccgg aaagcagcgg tagccctgat gaacaacctc aatctctcct gcaggctcaa
     7861 aagtaaacgg gaccaggggc gtttcagcac gccggtgtat tcactggcca gaaacgcctt
     7921 tatccgggtc atcaaaacag aaggcgcaca aaagtggctc aggcagcacc gttacgttcg
     7981 cagcggtctg gtctggcttt acgcacatga tttgcgcctg acgcctccga actggctgtg
     8041 gctcaagggc gttgaccgga cgctcttcta tgcccttcac cgggcaaaca ccacaaaagg
     8101 cttcatcgaa ggggccggcg tggtagccgt tgcccgggca gaaaatgaag cgtgtcgtct
     8161 cggcctgccc tgcccggaac cctgtgtgga agaagccatt gaagggctgc ggcaggacat
     8221 gctgagactg gggttgatat gggacgaacc tcagccagac cgcgatcgca gacgtcagat
     8281 aaggacccgc tggtcactca cggatgacgt gataccacgc cgccatgata acgatgaagg
     8341 ctcagatact ggtgaaacca ccgaaaccac tgaaaccact gaaaccactg aaaccactga
     8401 aaccactgaa accactgaaa ccactgaaac ccggcatccc gccgacaagg aaaaagcaca
     8461 atgaccatcc gctacactgc aacactgaac aataaacacc tgtccctgac cgccctgtac
     8521 cctgacggtg gtcaggaggt tattgccatc ctgccggtta cagaactggg cgcactgatt
     8581 aaccgtaata acaggctgat gcacctgtcc ttcagcaccc cttttcgtga aataaccgaa
     8641 acactgcttg ttgataccgt tgatactgaa cgtcccggac atcacatcgg cagaatgctc
     8701 tccgcgattg acagctgtct gagcgcggca tattcagaac ttcctgcccg gaaaaccagc
     8761 ttcatgagag ggctgatata cggtgcagga ttagtgctgg cacttctgac agcggtaaca
     8821 gccggatata ttttactgaa accggacagc gattcccctc tcagggtacc tgtcacagaa
     8881 tccgtaacag aaacagcacc attacacatc gacaaggaaa ttgtccggtc agtagccccg
     8941 tcagagacga aaaatgaaat cgttcacccg gcaccacagg aaatgcagga gacatccgct
     9001 accccggcga taacagcaga caaaaatact ctggtcgctg aagcccgccg taatctggcc
     9061 actgtactta aacgcaacgc ggaccggggt atgttcacca ttaatctctc cactggtcat
     9121 gaacgcacac tctacgcctt ccttgacccc gcatgcatca actgccgtct tctggagccc
     9181 gcgcttaagc agctggcccg ggaattcagc gtggtgattt atccggtctc cgtaatcggc
     9241 ggagaggcca gcacaaacca gactgcgcca ctgctgtgtg aaccggacgc acaaaaacgg
     9301 gcgaccggat ggcatcagct gtattccgca gacggtggta tggcacccac cggcgatgca
     9361 ccggctgcac cggatgaaaa gtgtctgacc gctgctcgtg ctgccatcga tgtcaataac
     9421 caggctttcc gccagttcgg ttttggcggt accccatggg tgttatccga taccggctgg
     9481 cacctgccgt ccggcatcct gaaagacgcc actaccgtca gcctgttcct gaaagccaca
     9541 gataccgagg ataatcacaa tgagccagca tcacgttaat cctgacctca tacaccgcac
     9601 ggcctgggga aacccgctgt ggaatgcact gcacaacctg aacatcattg gtctgtgtct
     9661 ggccggcagt atcattacgg ccctgatatg gccactggcc cttcctgtct gtctgctgtt
     9721 caccctggtc acctccgtga tattcacgct gcaacgctgg cgatgccccc tgcgtatgcc
     9781 catgacgctc agccttgatg acccctccca ggacagaaag gtcaggcgaa gcctcttcag
     9841 cttctggccc accctgtttc agtatgaagc agatgaaaca tcccccgcac gggggatttt
     9901 ttatgtcggt taccgccgta tcaacgatat cggtcgcgaa ctctggctga gcatggatga
     9961 cctcacccgg catatcatat ttttttccac caccggtggc ggtaaaacag aaaccacctt
    10021 tgcctggctg ctcaaccctc tctgctgggg aagaggcttt acctttgttg acggaaaggc
    10081 acagaacgat accacgagga caatctggta tctctcccga cgttttggcc gggaggatga
    10141 tatcgaggtc atcaacttca tgaacggcgg aaaatcccgc agtgaaatca ttcagagcgg
    10201 cgaaaaatcc cgcccacagt caaacacctg gaacccgttc gcattcagca ccgaagcctt
    10261 caccgccgaa accatgcagt ccatgctacc ccagaacgtt caggggggag aatggcagtc
    10321 acgggcgata gccatgaaca aagccctggt ctttggcaca aaattctggt gcgtccggga
    10381 aagaaaaacc atgtccctgc agatgttacg ggagcatatg acgctggaag ggatggcaaa
    10441 actctattgc cgtgggcttg atgaccagtg gccggaagaa gccatcgcac cactgagaaa
    10501 ttacctgcag gacgttccgg gattcgatat gtcactggtg agaacgccgt cagcctggac
    10561 tgaggagccc cgtaaacagc atgcttatct ctccggtcag ttctcggaaa cattcacaac
    10621 ctttgccgaa accttcgggg acgtctttgc ggcagatgcc ggcgacattg atattcgcga
    10681 cagtatccac agcgaccgaa ttctgattgt gatgatcccc gcactggaca catcagccca
    10741 taccacgtcg gcactcggtc gtatgttcat cacccagcag agcatgatcc tcgcccgtga
    10801 cctgggctac cgcctggaag gcacagacgc acagaccctg gaagtcaaaa aatacaaagg
    10861 ccgcttcccc tatatctgta tccttgacga agtcggtgcc tactacacgg accgcattgc
    10921 agtggaggcc acccaggtac gttcactgga gttctccctg ataatgacgg cgcaggatca
    10981 ggagcgaatc gaagggcaga cttctgccac caacaccgcc acgctgatgc agaacgccgg
    11041 caccaaattc gccggacgca ttgtcagtga cgacaaaaca gcaaggacgg tgaaaaatgc
    11101 cgccggtgaa gaggcccgcg ccagaatggg cagcctgcag cgccacgatg gtgtgatggg
    11161 tgagtcctgg gtggacggta accagatcac aatccagatg gaaagcaaaa tcaacgtgca
    11221 ggatttgatc agactgaatg ccggtgagtt ctttaccgtt ttccaggggg atgtggtgcc
    11281 gtcagcctct gtgtatattc cggacagcga gaaaagttgc gacagtgatc cagtggtcat
    11341 caaccgttat atctccgttg aagccccccg actggagcgt ctccgccgtc ttgtccccag
    11401 gacagtgcag cgcaggctcc ccacaccgga gcatgtcagc agcattatcg gtgtgctgac
    11461 agaaaaaacc tcccggaaac gtcgtaaaaa acacacagag ccttaccgaa tcatcgatac
    11521 cttccaggac cagcttaaaa gaacacaaac attgctggat ctgcttccgc aatatgatac
    11581 agatatcgaa tcccgggcaa acgaactgtg gaaaaaggct gtgcacacta tcaataacac
    11641 aaccagggaa gaacgccggg tatgttatat cacactgaat cgtccggaag aggaacactc
    11701 tggtcctgaa gatatacctt cagtgaaggc gattctgaat actctgctcc ccctggagat
    11761 gctgttacct gtaccggatc taactgcatc cccgccccat aaaaaaaatg tcgctcagac
    11821 accatcaggt ggaaaacaag agagtcgtaa aaaaagattc tgataacacc tgtatggctg
    11881 attttaataa caacctttac gcgattatta atctcctccg ctcaccatag taagcggagg
    11941 attcaggatt tcaatccatc accagaggta caataaatgt gtattagtag cccggaaaca
    12001 aactcatggt ctgttattta tcgtaaaaat tccggagaag atatcaatat cacttccctg
    12061 acgtttaaaa atagtcttct ggcagcccgg acactgatgg tgcccgaaaa ctatattata
    12121 tgcatactca gaaacgggga gagggtcaga cgatgggaca gagaaatact tgcaggctca
    12181 aacagatggt acaagtgttc tcccgataat ttcgaaatac tgggtaaact ccccataata
    12241 aataaagtca caacactgat aaaatcataa tctacggatt ttaatcagtt aagctaaatc
    12301 aggatgaacg ctttgattat gaattataca tgaaaacaaa tatgggaagc acaaaaaatg
    12361 gtgccagggt tccccacgtt cgtaatagat tcacttctat ggatattggt atgaattccc
    12421 agatcctgcc agacatgctg attaattttg cccttattaa catcacagac agaaagaacg
    12481 agggtaccaa taccattgat ggtaactggc aggccgatga aggacgccgt taccgggata
    12541 atgtaagaat ctatttcagc caacgccctt agctcgtttt ctgaaaaagt cgtcctgaag
    12601 tcatgtatct gcttccattc tcatgttcat atttctcctt acgctttcac tgtctgtcat
    12661 cggaagtacc gatttatttt agtgctgctc acttacagcc attcacggaa gaggattgtg
    12721 gtgaacagta ggagtctggc tgctggcgag ggaaggttca ggactggtat ggggtttagt
    12781 gctggaggat gactttgaat cccggactgg tcagtgaggt gatgatctgg tgtaactcaa
    12841 tctgccaggg taaactctcc ttgcttttgc gcacaagcag tgggccctgg ttcgcttatg
    12901 tcctgcatct acaagcgaat gactgaccgg agactcccat atctggtcag tcgcccattt
    12961 ttattttcta ccaaatcaga caagcatacc gtaataaata acgccagtcc cctgtcactc
    13021 tcgtgtgctc tactggaatg ttgttgatgg agatatccag atgaaagagc ttacatcttc
    13081 gacaggacta taaattatgc agtaaagagt tccccggaga aaggggctgt tctgctgttc
    13141 tcatcaagtc gggtatgttc aggaaattat tcgaaaatgt taactgaatg agcgaaatgt
    13201 gatttacctt cttttctgtt cattgtaaat tccctctttg ctaacgcaaa tcattatcat
    13261 tacgcctttg gcaaagggaa atttatgaag aataaatata tcattgctcc gggcattgcc
    13321 gtgatgtgtt ctgcagttat atcatcaggt tatgccagtt ctgataaaaa agaagatacg
    13381 cttgttgtta ctgcctccgg gttcactcag cagctcagaa atgccccggc cagtgtctca
    13441 gtcattactt cagaacaact gcaaaaaaaa ccggtttcag atctggtcga tgcagtaaaa
    13501 gatgttgaag ggattagtat cactggtggg aatgaaaaac cggatatcag tatacgtggt
    13561 ctaagtggcg attacacgct gattctggtc gatggacgac gtcagagcgg tcgggaatcc
    13621 agaccaaatg gcagcggcgg ttttgaagcc ggatttatcc ctcctgtgga agcaattgaa
    13681 cgcattgaag tgatccgtgg ccctatgtct tccctgtatg gttctgatgc catcggaggg
    13741 gtcattaata tcataaccaa accagttaat aaccaaacat gggatggcgt acttggactt
    13801 ggggggatta ttcaggaaca tgggaaattt ggtaactcaa ccacaaatga cttctatctg
    13861 tcaggcccat tggttaagga taaacttggt cttcagctat atggaggaat gaactatcgc
    13921 aaggaagata gtatctctca gggaacaccg gcaaaagata ataagaatat aacggcaacg
    13981 ctccagttta ctccgactga aagccagaag tttgtttttg aatatggaaa aaataaccag
    14041 gtgcatacat taacacctgg tgagtctctc gatgcctgga ctatgcgggg aaatcttaaa
    14101 caaccaaaca gtaaaagaga aactcataat tcacgtagtc actgggtagc agcatggaat
    14161 gcccagggcg aaatactgca tcctgaaatt gctgtttatc aggagaaagt tattcgtgag
    14221 gttaaatcag gtaaaaaaga taaatataat cattgggatc ttaattacga gtcaagaaaa
    14281 ccggaaataa ccaacacaat catagatgca aaagtgacgg catttctgcc ggaaaatgta
    14341 ctgaccatcg gaggtcaatt tcagcatgca gaactccgtg atgactcagc cacaggtaaa
    14401 aaaacgacag aaacacagtc tgtttcagtt aaacagaaag ctgtttttat agaaaatgaa
    14461 tatgctgcaa cggattctct cgctttgact ggaggactgc gtctcgataa tcatgaaatc
    14521 tatggcagtt actggaatcc aagattgtat gctgtttata acctgaccga taatctcact
    14581 ctcaaagggg ggatcgcaaa agcatttcgg gccccttcga ttcgtgaggt cagtcctgga
    14641 tttggaacac tgacgcaggg tggcgcctct attatgtatg gtaacaggga cctgaaaccg
    14701 gagaccagtg taaccgaaga gatcggtatt atttataata atgaccgtgg tttctcagca
    14761 agtgcgactc tgtttaatac agattttaaa aataagctga ccagttacga tataggcaca
    14821 aaagatccgg tcactgggtt aaacactttt atttatgata atgtgggtga ggcaaatatc
    14881 agaggtgtgg agcttgcaac tcagattcct gtgaatgata aatggcgtgt atctgcaaac
    14941 tatacattca ctgactcccg tcgagaaagc gatgacgaaa gtctcaatgg caagtcgctg
    15001 aaaggggaac ctctggaaag aactcccaga catgcagcca atgcaaaact ggaatgggat
    15061 tacactcagg atattacatt ttattcatct ctgaattata cgggaaaaca aatctgggca
    15121 gcacaaagaa atggtgctaa ggttccccgc gttcgtaatg gattcacatc tatggatatt
    15181 ggtctaaatt accagattct gccagacatg ctgattaatt ttgccgttct taacgtcaca
    15241 gacagaaaga gcgaggatat cgataccatt gatggtaact ggcaggtcga tgaaggacgc
    15301 cgttattggg ctaatgtaag agtatccttc tgagagtata taaagataac ctgaggccgt
    15361 gagcctcagg taccagtgta gtaatcagta acgtcagttt ccaggttgta atgcttttaa
    15421 tcttgattta aataagctta ataaattaca cttgttaaac atggatattt ttaatttttg
    15481 agtatgtcaa cataacctga ggcaaagtac ctcaggttcc agtgtagtaa tcagccaacc
    15541 tgacagtctt ctgttagccc tacagatatg tcgtggagct aaccgcgcat gtcaaagccg
    15601 gatttttgaa aatctctata aacttccgga ttgttgaagg ccgggaattt agctttatgg
    15661 gctgctgact ttatcgcttc gcaataggct ttatcgccat tactggttga tatttttaac
    15721 gccgtgccat cccgggagaa ttccatatgc aacctgcatt ttttcctttt ccatttttgc
    15781 ggatcatcaa gtttagcatt aattgcatcc ctgattcccc gtgcctgctc cccccattca
    15841 tcctgatcat cccaacgtcc tgaactgcaa ctacccaggg ctgtagtttt atggcaattt
    15901 gaaggatgta atggcgcaca tcccgccaca aagctgacta aaatagccaa cataacgatt
    15961 ttctttaccc ccatatattg ctcctcaatc catatagatc tcagtaccat agctctgtca
    16021 gagcaaagaa aatcttaact aatacaagtt aagattttta atatccactc ctcatcccta
    16081 cattccaggt gatggtggcg tccactcatt atcctgaact ggagcataag atgcgccatg
    16141 ccccccggat atgcttttct gttgcctgat atattcgtgg tagtactgtt cactacgctg
    16201 acgatgtaaa tccacatccg ggcggctgat tacaaagtgt ccatttccgg ttattccagc
    16261 tgcattatgc caggccacca tttccgcaaa tacatcatta aactgttgat atgagccttc
    16321 cggttcaaaa cccattcctg gctcacgatc aaacaataca ggagcaatca gattcagctt
    16381 atccaccagt tcatttctgt cctgatgatg gtagaacacc acacggtccc cataatccac
    16441 acagactaac tcgccattcc tccgatcacg aaaacgcaca ctgccatctt tctgcagacg
    16501 ggcttcgaga acgccagtgt cttcataaag tggagttcct cccggttcac agagaatcac
    16561 gcagcggtcg gcattcgttg ttcgtcgctt gtctttgcga cgatcacgat aatcccagcc
    16621 acgtaactgt gataatgcag ctctgtctcc ctgtaccgcc tgttgctcca cccactgccg
    16681 gtaggacaaa ggataccact taccctccgc aatcagtgac aaccgctctt ccttcacact
    16741 ctccttcagc ctgatcagcg cctgcatacg ctgcacttcc gcaatatggt aatgcagctt
    16801 acgcaattgt ggatcccgga actgcacacg gatatacgcc ttgcgccgac ggcaggcagc
    16861 atgaatttcc cgtaaccgtt ctccgtaacg taagtcgggt ttacgccagt gctccttcca
    16921 tgcaagataa cgcgcgcgca aatcttcccg cgcagctgca cgggcttcac ggcgttcacg
    16981 acgcagtaca ggatcgcgcc ggaagccggg ctccacttca tcgctggcag caagttccgg
    17041 attgtagcga cactcaggct tcacccgttc aaatatatct gcacctgcaa tctcaaatgg
    17101 ccctgcctgc ggctcagccc tggataacgt caaatccggg tggatactgc tggccttcac
    17161 tggtgtgagc tcatgattaa acgcatccac aatcacaaga ccatgatgct gtttctgcaa
    17221 cataagccct ttacgcgcaa acatctcatg gcacgcctgc cagctgaaat tcccgcccgc
    17281 gatcatatct tcaatcaacg actcacactg aacagtcgtg tacccccaca gactttcctt
    17341 atcagaaaac agttcgcgct ttattgctcc ctgtggacct gtccccatcc gcatacgata
    17401 ccattcgtaa tcactgacag cctgagtacc aagattacgt ccccggaatc gttctccggg
    17461 tataacctga gtaaaaatat ccttcggtac aggctgccat ccaccatcca gatcctgcat
    17521 ataatccagc gtcattaacg gccacacact gtctgcacgt actggtgtct gttgtttatc
    17581 aaatccatca tgcagtacca gatgcccgtg ctggatcccc atccataatc cagcctttgc
    17641 cagagttctg tgcacatcca ggcaactttc cagctcacca tttctcgcca tttccggtaa
    17701 gcgttcggca aaatgacggc aggcgtactg tttcagactc tccgtttctg ccactttttc
    17761 cggccggata tcaacgttta tggcttccgg atcataacga ccgggtgtcc ccacctgact
    17821 gaaaatatct gtcggtacag gctgatattc ccccagtttc cgccccagtt tttcagctgt
    17881 ccatgacggt ccgaacgaac tgagtgcaac accttcccgc gctctgtccc agccatcctt
    17941 caccaccagt tccccttcct gcatcgtgat atacagacca tcactggcca gccgtttatg
    18001 caaagataac cagtcctgtg cgggctcttc acgcagcccg gcaatcgaca tctgagcaat
    18061 atattccctg aacgtctgct ttttcccgcg tcgccaggca ttacggcgct cacgtaccag
    18121 cgctgttttg cgcacaatac ggttgccggg tgcatgcaca aagcagccat tatcaggcgc
    18181 gaatccgtgc ttaagttcaa gttcacgaca tgcacggctg agtttttcct ggctccagga
    18241 aagccagtta atataaccag tttcaggatg tacacgattc acggcaacat gcacatgcag
    18301 attatccgtg tccgtatgaa cggcagccac gtactgatgt tttgagagtt caagagattt
    18361 cagtgtgtga cgcacacagt caaaaagctg ctcaggacgc gggctttcat gcgcaggcca
    18421 ggaaagaatg tagtggaaaa cggggtctgt atcattacgg gaaaaaacag ctttgtccgc
    18481 cttgtactgc atctcttcag cagctgtttc cagtgaattg cagttatgga aacaggttac
    18541 gccatagaaa tttacccact gactgccgtc tttcatcaca tcaatcaggg agacaaattt
    18601 ttcgttacgc aggcgtgttg cataatcaac aagccgtctg aaacggttgc ggtgagggac
    18661 atcactcttg atttcgctgt cttctgtgag ctcattttcc tgcacatcat cccgcacaga
    18721 cgtgtacgca atcaggtctt caaaggaaga cctgccgtca cgtcttttgt gcggaataat
    18781 ggcattcatc agcagttatc tcagcgtccc attaagtctc ttcgcagagc attgacatat
    18841 tcagtaatgg caacaagaac ctctgcatac tccttatcac cagtgcgttt tccctccaca
    18901 aacagatgct tctgaaggcg gccaagacgc ataatctcat caatcatacg gtcgtcagta
    18961 cggcagttaa ccaccttatc cagtgaagag cttcgtataa atgttgaaac cgtctgtcca
    19021 cttgactccg ctttttctct tacgatttca gcctcatcct ccgtgaatct cgccgtgaat
    19081 tttacaattc tcttcctgtt ctcactgccc gttcgggttt tcttctcact catggtctgc
    19141 tccttaccgg ggtgtcgggg caaagccctg accaggtggg gaatgtctga gtgcgcgtgc
    19201 gcggtccgac attcccacat cctgtcccgt ttttcaggcc attatagcct gttatttttg
    19261 tgcatcatag tcttatgcag acggcagagg cagggcgcag ccctgccagg aggcagtttt
    19321 tgatgataaa atcagacttc aacatgaaac ggagagaaat tgccaatggc ttctaaaaaa
    19381 ttttactcag atgatgacat ccagctggca aaagctgctt tatcagaatt gccggacctc
    19441 accgctcaac ggaaaacatt gcgtgatttt cttgatgcca ttcgtgatga catcattgtc
    19501 ctggtaagga caaagggtta tacactggca gatgttcgtg acacgctcaa aaatgcaggg
    19561 tatgaagtag gagagaaggc acttcgtgac attattcggg aagcagaatc gaaaaaaaca
    19621 tcccgccgtt cttcctctaa aacagcacct aaaaagacag attcagccag gaaagatggc
    19681 atcgatatga acaacaattg atgtgacaat gtcacatcgt attctgagat tatgatgatt
    19741 acagagaaaa agccctggca taaataccag ggcttttctg tttaatcaga ctcgtggcgg
    19801 caatgcacca ggataatgcc gtgcaataat atcattcagt ttttcaacaa ggtcaggacg
    19861 cttaaagaca atgtgtacgg ttcctttctg gtagccacgc accgtaaaat attccccttc
    19921 taacagctta ccgacattgt ctccgttccc gagaaattca ccatactgcg caccgatgct
    19981 gctccggaag tcaggaacgt tgcgcccctc cagcagataa aacggacgta ccaggtcatc
    20041 cagtgcctcc cgtccgctgc agtccagcgt cacacgcccg ttactccagc ggtacagcag
    20101 gttttcaatg atgattttct tgcccagacg gcagggatta ttggttctgt aatcccatga
    20161 caatttccgc cagacatcaa tcagtccctg ttcaaatgtc tgcatcttac tggcattcag
    20221 atggcgaaaa gttgccagta cattgtccag gattatttca ggacaggtgt cgctcatcag
    20281 ctggctgttc cactcatcac gctgacatga actcatgaag gtgtacatcc ccgtatccgt
    20341 catcaggcgg tcccagattt tccggtcggc aagacgggtg atattccttt tcgcttcttc
    20401 cggtttatcc atgcatcgaa cggagtcagt cacgcagtct tcaaacccgt agagaaaaat
    20461 gtcaccggaa gcatcgagca gaatcctgcg tgctgtcgcc aggcattcca tcgcctgcat
    20521 gaacagtgaa ataccttcgt tacgctgcgc gatgatgcgg tcgatagcca cagacggaat
    20581 aacatctgtc gtgccgtcag tattcaggtc agcagtgggt aaagtctgat tcatcacatt
    20641 gtctcctcat cgtcacgact gccgggtcag cctggtcatt tcccggagga gcgaagctcc
    20701 ttctgccgtc ctggtcttcg tggatttccc cgcagtggac ctttccgacg ggggacgctc
    20761 accacgtcac ggggcagttg tccgggggca gacgaaaaag tttttgacgc ctgcggcaaa
    20821 aagttttgtg gctggtacat ctctcccctg gacgactgaa acgggatgtg gtcgtgtatg
    20881 tacccccgga aagtccgtct gcggagaaca ggccgcagtg cggcggcaga caggcagaga
    20941 cagctgcgcc atcaggcgca gtgaccttac acaccgtcag gtgtgcaggg agcgccgcat
    21001 ccggcgcgac tgaagaaaaa cgatggcaca acaatggcat gtggtggtca ctgacgcacc
    21061 actcccgcac aggtgaaaac agagaaaacc gcacgacatc tacaataaaa aaagtgaaat
    21121 ttcttcactt tttcacactt atttatcgcg ctgatttggt gattatgatg ataaaataaa
    21181 gtgaagtttt ttcatttttt caaaggtgtt ttatgaccgg ctgggaactg cgaatctggc
    21241 gaaaaagcat gctctggtca cgcgaaaaag cggccagaga atttggcgtg acgcagagaa
    21301 catggcacgc ctgggaaaat gcggaacagg tggacgtcac cgtctggagg accacccagg
    21361 cactttcagt ccgtgacctg ctgcctcaca tgcaggggat gcgcaaggca gatatcatca
    21421 ggcgtctgga aaatgaactg ggggaaacgg cagggaatgt gtgagcgtga ttcacagcct
    21481 ggctgaatgc caggctactg ctcatcagga aacagctttt tcagttcttc accggaaagt
    21541 cccgtgacct cctgaatccg ttccggcggc cagcctatat cccgaagccg gcaggctatt
    21601 tcccgcgcca cttcaagagc tccttgttta aaaccttgtt taaaaccttc tttaaaaccc
    21661 tcgtcgaaac ctttctgtat aagacgttct gcaatcgtca tgctgtttgc tctgctcctg
    21721 ctggcaaaaa gcccggcatt ttgccgggct gtgatgctct catcagtgaa tgactttttt
    21781 cagttcagct tcggaaagtc cggtggcctg cttaatctgc tcaggtgtca tccccatttt
    21841 ctgaagctga cgcgcaatgg ccagggcttt ttcaagcgca cctttctgct caagacgttc
    21901 tgcgattgtc atcagtttat cctcatgctg cggcaggcgc tc
//
LOCUS       DAZGAN020000076        21935 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGAN020000076 DAZGAN020000000
VERSION     DAZGAN020000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.076"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..530)
                     /locus_tag="ACHKYO_RS15990"
                     /old_locus_tag="pgaptmp_003198"
     CDS             complement(<1..530)
                     /locus_tag="ACHKYO_RS15990"
                     /old_locus_tag="pgaptmp_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030598 - rRNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-inactivating family protein"
                     /protein_id="HGU4429999.1"
                     /translation="MKCILLKWILCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIVEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDAS"
     gene            complement(622..698)
                     /locus_tag="ACHKYO_RS15995"
                     /old_locus_tag="pgaptmp_003199"
     tRNA            complement(622..698)
                     /locus_tag="ACHKYO_RS15995"
                     /old_locus_tag="pgaptmp_003199"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(662..664),aa:Arg,seq:tct)
     gene            complement(712..788)
                     /locus_tag="ACHKYO_RS16000"
                     /old_locus_tag="pgaptmp_003200"
     tRNA            complement(712..788)
                     /locus_tag="ACHKYO_RS16000"
                     /old_locus_tag="pgaptmp_003200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(752..754),aa:Arg,seq:tcg)
     gene            complement(798..873)
                     /locus_tag="ACHKYO_RS16005"
                     /old_locus_tag="pgaptmp_003201"
     tRNA            complement(798..873)
                     /locus_tag="ACHKYO_RS16005"
                     /old_locus_tag="pgaptmp_003201"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(838..840),aa:Ile,seq:cat)
     gene            complement(914..1972)
                     /locus_tag="ACHKYO_RS16010"
                     /old_locus_tag="pgaptmp_003202"
     CDS             complement(914..1972)
                     /locus_tag="ACHKYO_RS16010"
                     /old_locus_tag="pgaptmp_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGU4430000.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLASQNG"
     gene            complement(2123..2320)
                     /locus_tag="ACHKYO_RS16015"
                     /old_locus_tag="pgaptmp_003203"
     CDS             complement(2123..2320)
                     /locus_tag="ACHKYO_RS16015"
                     /old_locus_tag="pgaptmp_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4430001.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(2547..3368)
                     /locus_tag="ACHKYO_RS16020"
                     /old_locus_tag="pgaptmp_003204"
     CDS             complement(2547..3368)
                     /locus_tag="ACHKYO_RS16020"
                     /old_locus_tag="pgaptmp_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4430002.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDCVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCSCCHGKKFIESEVFTNKIQYPDSKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVSKFDIEEAWAERQLKKVTR"
     gene            complement(3365..3745)
                     /locus_tag="ACHKYO_RS16025"
                     /old_locus_tag="pgaptmp_003205"
     CDS             complement(3365..3745)
                     /locus_tag="ACHKYO_RS16025"
                     /old_locus_tag="pgaptmp_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4430003.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFISEEGKRYLRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLIDDEQFDEINIVR
                     GKPVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(3746..4804)
                     /locus_tag="ACHKYO_RS16030"
                     /old_locus_tag="pgaptmp_003206"
     CDS             complement(3746..4804)
                     /locus_tag="ACHKYO_RS16030"
                     /old_locus_tag="pgaptmp_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4430004.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISSTQERE
                     LSLAELSWWAVCNQVVDALPEAVVRRSLGLPAEKIRSVYRESDIIPGEQAATSILKQR
                     TKNIALPLHVHQQQNPPQEKTLVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            complement(4806..5084)
                     /locus_tag="ACHKYO_RS16035"
                     /old_locus_tag="pgaptmp_003207"
     CDS             complement(4806..5084)
                     /locus_tag="ACHKYO_RS16035"
                     /old_locus_tag="pgaptmp_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430005.1"
                     /translation="MAQDTKLHNSDNSTAPASRHGRRSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            5220..5477
                     /locus_tag="ACHKYO_RS16040"
                     /old_locus_tag="pgaptmp_003208"
     CDS             5220..5477
                     /locus_tag="ACHKYO_RS16040"
                     /old_locus_tag="pgaptmp_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU4430006.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRVAIRSGK"
     gene            5483..5782
                     /locus_tag="ACHKYO_RS16045"
                     /old_locus_tag="pgaptmp_003209"
     CDS             5483..5782
                     /locus_tag="ACHKYO_RS16045"
                     /old_locus_tag="pgaptmp_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4430007.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNIIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGRKVFIVGIYHGGQSYETDFL"
     gene            complement(5985..6515)
                     /locus_tag="ACHKYO_RS16050"
                     /old_locus_tag="pgaptmp_003210"
     CDS             complement(5985..6515)
                     /locus_tag="ACHKYO_RS16050"
                     /old_locus_tag="pgaptmp_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4430008.1"
                     /translation="MSTITREWLQQAINDYESVRDELPFGLDDYQGNILASLRIALAS
                     LEAEPVAWKVTFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAKPVP
                     VTPDGWISCSERMPDTKTAVLVAREFDRKGDWRMKWATYIPGHPDANDGWVVPGASWI
                     PSHWMPLPEPPQEVRQ"
     gene            complement(6512..7189)
                     /locus_tag="ACHKYO_RS16055"
                     /old_locus_tag="pgaptmp_003211"
     CDS             complement(6512..7189)
                     /locus_tag="ACHKYO_RS16055"
                     /old_locus_tag="pgaptmp_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4430009.1"
                     /translation="MSKIDYQALRAKAEKATCGEWSLEYGEGRFDGDDALIHREVAGY
                     IPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDERERNQQYIKRRDQE
                     NEDIALTVGKLRVELEAAEKRIAELQARDVKPVAWMRNANVTSFMSRFTTDEKYAVEQ
                     WGDDAVALYPLPVAFIPACFTDERNLMHINERGRETSLIWSKQNSDSGDIKLFRIAAA
                     AGKGEES"
     gene            complement(7186..7749)
                     /locus_tag="ACHKYO_RS16060"
                     /old_locus_tag="pgaptmp_003212"
     CDS             complement(7186..7749)
                     /locus_tag="ACHKYO_RS16060"
                     /old_locus_tag="pgaptmp_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4430010.1"
                     /translation="MRKINYQTLREIAKLATQGEWVAFISPGTDTYAVHTPGDERCGD
                     IIKWPGFDDQKNAENNAEFIAAFNPEVVQALLDEREAQSKRIAELEANLVALAAENAL
                     ARKAVQAFCDVVGDNTEVICEEVGRDGVLVILEAMKATGNMPATDAFLAEVRAQGVEM
                     FAECAYTLEHHDHAVAFAAELRKGGNQ"
     gene            complement(7736..8041)
                     /locus_tag="ACHKYO_RS16065"
                     /old_locus_tag="pgaptmp_003213"
     CDS             complement(7736..8041)
                     /locus_tag="ACHKYO_RS16065"
                     /old_locus_tag="pgaptmp_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGU4430011.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHRAEEALKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEKN"
     gene            complement(8038..8319)
                     /locus_tag="ACHKYO_RS16070"
                     /old_locus_tag="pgaptmp_003214"
     CDS             complement(8038..8319)
                     /locus_tag="ACHKYO_RS16070"
                     /old_locus_tag="pgaptmp_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4430012.1"
                     /translation="MILRQCAGTMTVESIGSLIGRSGAAVRTKARELGICMMLRGDYH
                     QSAKCSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRRVQA"
     gene            complement(8352..9113)
                     /locus_tag="ACHKYO_RS16075"
                     /old_locus_tag="pgaptmp_003215"
     CDS             complement(8352..9113)
                     /locus_tag="ACHKYO_RS16075"
                     /old_locus_tag="pgaptmp_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4430013.1"
                     /translation="METVFDALKAMGKASSVGLAERLDISREEVLNELWELKRNGVVD
                     KAGHIWFLAGEGESGVTEEQPEQSEVPDVLTGEVEQKVTADMMIEFIGQEGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAVPVTENDGKA
                     FPQPAGVALPVQESATQEDIKTETVADIVQSLPSFTETQADELIFLSLCRANLALRRA
                     KSDVQKWERVCAALRELNKHRDILRDITATRELLR"
     gene            complement(9147..9608)
                     /locus_tag="ACHKYO_RS16080"
                     /old_locus_tag="pgaptmp_003216"
     CDS             complement(9147..9608)
                     /locus_tag="ACHKYO_RS16080"
                     /old_locus_tag="pgaptmp_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4430014.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGSGMSVPPVRRQLEGAKHPKGPTPIEWLKQEYERRKAAGFI"
     gene            complement(9601..10638)
                     /locus_tag="ACHKYO_RS16085"
                     /old_locus_tag="pgaptmp_003217"
     CDS             complement(9601..10638)
                     /locus_tag="ACHKYO_RS16085"
                     /old_locus_tag="pgaptmp_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HGU4430015.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKYDADCHDESRRVTHLSRQVTTDKDTDTE
                     LNPTHNARMRESAPTGESNGASLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAA
                     VWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVS
                     HAASGGTASRAIQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTF
                     EALDCPDWCDD"
     gene            complement(10707..11132)
                     /locus_tag="ACHKYO_RS16090"
                     /old_locus_tag="pgaptmp_003218"
     CDS             complement(10707..11132)
                     /locus_tag="ACHKYO_RS16090"
                     /old_locus_tag="pgaptmp_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4430016.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTSDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAVAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(11116..11388)
                     /locus_tag="ACHKYO_RS16095"
                     /old_locus_tag="pgaptmp_003219"
     CDS             complement(11116..11388)
                     /locus_tag="ACHKYO_RS16095"
                     /old_locus_tag="pgaptmp_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4430017.1"
                     /translation="MLDSTRKKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVECQANTQPETGVIHENQA"
     gene            11497..11898
                     /locus_tag="ACHKYO_RS16100"
                     /old_locus_tag="pgaptmp_003220"
     CDS             11497..11898
                     /locus_tag="ACHKYO_RS16100"
                     /old_locus_tag="pgaptmp_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4430018.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVTGHPLHWFLLPPEEGEQMFTPNTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            11926..12117
                     /locus_tag="ACHKYO_RS16105"
                     /old_locus_tag="pgaptmp_003221"
     CDS             11926..12117
                     /locus_tag="ACHKYO_RS16105"
                     /old_locus_tag="pgaptmp_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HGU4430019.1"
                     /translation="MSTALSPIVSEFETIEQENSYNEWLRAKVASSLADPRPAIPHDE
                     VIAEMENLIAQIAVTNRSE"
     gene            12117..12404
                     /locus_tag="ACHKYO_RS16110"
                     /old_locus_tag="pgaptmp_003222"
     CDS             12117..12404
                     /locus_tag="ACHKYO_RS16110"
                     /old_locus_tag="pgaptmp_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4430020.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHARRQFPFSI"
     gene            12678..12830
                     /locus_tag="ACHKYO_RS16115"
                     /old_locus_tag="pgaptmp_003223"
     CDS             12678..12830
                     /locus_tag="ACHKYO_RS16115"
                     /old_locus_tag="pgaptmp_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4430021.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
     gene            13326..14180
                     /locus_tag="ACHKYO_RS16120"
                     /old_locus_tag="pgaptmp_003224"
     CDS             13326..14180
                     /locus_tag="ACHKYO_RS16120"
                     /old_locus_tag="pgaptmp_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4430022.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKAGAGRGNPMMTTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQPKGWPVSVVTGISTPVRLTTLMVVESLGGELFKLTIEAAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHERVLDKIRNLDCSAKFTEHNFVSSEYT
                     DSTSRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKELRQNNAPSPDKM
                     IHGDGRTLVIRLDEHGNIKFTETVPDGAMVCTLDTFQFYLEKQGWTLVNRSAIKNMTV
                     EQLLKIHC"
     gene            14191..14379
                     /gene="dicB"
                     /locus_tag="ACHKYO_RS16125"
                     /old_locus_tag="pgaptmp_003225"
     CDS             14191..14379
                     /gene="dicB"
                     /locus_tag="ACHKYO_RS16125"
                     /old_locus_tag="pgaptmp_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4430023.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            14376..14564
                     /locus_tag="ACHKYO_RS16130"
                     /old_locus_tag="pgaptmp_003226"
     CDS             14376..14564
                     /locus_tag="ACHKYO_RS16130"
                     /old_locus_tag="pgaptmp_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4430024.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCLRARDEQKISGE
                     CLPLKKVSLYLNNEIPAG"
     gene            14657..17101
                     /locus_tag="ACHKYO_RS16135"
                     /old_locus_tag="pgaptmp_003227"
     CDS             14657..17101
                     /locus_tag="ACHKYO_RS16135"
                     /old_locus_tag="pgaptmp_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4430025.1"
                     /translation="MSTDKEEFSLYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     VVAMDEAGYDADDFKKPVRVHFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQDKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVLRISRTPSGANAGGGILTDRGEGFVHDDASVE
                     RDVAAGVLARSMDIDIYNPHPAHAKRIEEIVSENKPPFSVFRDKFIAMPGHLDYSRAI
                     VVASVKEAPIGIEATPHRVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTKE
                     GKQDDEEKPQPSGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAE
                     LYEARKNIPPKNPVDADKLLAASHGEFVEGISDPNDPKWVKGIETRDSVNHNQQETEQ
                     KGHKAEQHSPNAQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPDCGAVMGDATYQ
                     ETFNEESQDEAREKDPEEMESAGLPNKECTEGDQHANGNNETGETANPLIKVNGHREI
                     TSTSRLWHHLMIDLETMGKNPDAPIASIGAVFFDPQTGEQGPEFSKIIDMGTCGGTVD
                     ISTIEWWLQRSGEARAAILADRIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNVI
                     LRRSYERQEIPCPWRYTNDRDVRTMVALGLVMDFDARNVTTFEGERHNALHDARYQAK
                     YVSAIWQKLFPNQADF"
     gene            17160..17363
                     /locus_tag="ACHKYO_RS16140"
                     /old_locus_tag="pgaptmp_003228"
     CDS             17160..17363
                     /locus_tag="ACHKYO_RS16140"
                     /old_locus_tag="pgaptmp_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGU4430026.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCRNTLPPREAEPDFGAI"
     gene            17363..18388
                     /locus_tag="ACHKYO_RS16145"
                     /old_locus_tag="pgaptmp_003229"
     CDS             17363..18388
                     /locus_tag="ACHKYO_RS16145"
                     /old_locus_tag="pgaptmp_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4430027.1"
                     /translation="MGRRRKNPEHEKLPPKVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNHHDHAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVL
                     ADSVKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPVPVFIAMEIAYLCAARISDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWTPRLQSVIEKAKQLPKSAYVISNQYGNRYMYKGFNEMWVEARNLAGQIS
                     GIATDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKISPTLDVPLPQN
                     IPTKYSK"
     gene            complement(18441..18530)
                     /locus_tag="ACHKYO_RS16150"
                     /old_locus_tag="pgaptmp_003230"
     tRNA            complement(18441..18530)
                     /locus_tag="ACHKYO_RS16150"
                     /old_locus_tag="pgaptmp_003230"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18494..18496),aa:Ser,seq:cga)
     gene            18624..19421
                     /gene="mtfA"
                     /locus_tag="ACHKYO_RS16155"
                     /old_locus_tag="pgaptmp_003231"
     CDS             18624..19421
                     /gene="mtfA"
                     /locus_tag="ACHKYO_RS16155"
                     /old_locus_tag="pgaptmp_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HGU4430028.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            19522..19597
                     /locus_tag="ACHKYO_RS16160"
                     /old_locus_tag="pgaptmp_003232"
     tRNA            19522..19597
                     /locus_tag="ACHKYO_RS16160"
                     /old_locus_tag="pgaptmp_003232"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19555..19557,aa:Asn,seq:gtt)
     gene            19910..21935
                     /gene="yeeJ"
                     /locus_tag="ACHKYO_RS16165"
                     /old_locus_tag="pgaptmp_003233"
                     /pseudo
     CDS             19910..21935
                     /gene="yeeJ"
                     /locus_tag="ACHKYO_RS16165"
                     /old_locus_tag="pgaptmp_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         5588 a         5766 c         5087 g         5494 t
ORIGIN      
        1 gatgcatctc tggtcatggc atttccacta aactccatta acgccagata tgatgaaacc
       61 agtgagtgac gactgatttg cattccggaa cgttccagcg ctgcgacacg ttgcagagtg
      121 gtataactgc tgtccgttgt catggaaaca gttgtcacac cgggcactga tatatgtgca
      181 aaatcagaaa atctgtagaa agtatttgtt gccgtattaa cgaatccggc cacatataaa
      241 ttattttgct caacaatcag acgaagatgg tcaaaacgcg cctgatagac atcaagccct
      301 cgtatatcca cagcaaaata actgcccggt ggggtatggt taataacaga caccgatgtg
      361 gtcccctgag atatatgctc aagaggagtc gatatttctg tccgtatact atttaacgaa
      421 gagacataac tttgttgagt cgaaaagtct atcgtaaatt cccgggaata ggataccgaa
      481 gaaaagccca gcaacaggca cagtatccat tttaacaata tacacttcat atacaggtgt
      541 tccttttggc tgaagtaata cggcaccaga cccggcgcag atataaaaaa ggcccgcaaa
      601 agcgagccag aaaaataagt atggcgcgtt gtactggatt cgaaccagtg accgattgct
      661 tagaaggcaa ttgctctgtc cggctgagct aacaacgctg aataccgata atggaccgcc
      721 atcgaggact cgaaccccgc gcaaccagct tcgaaggctg acgctctgtc ccgatgagct
      781 aatggcggta tgtgatatgg tggcccttgc tggatttgaa ccagcgacct ggcgattatg
      841 agtcgctcgc tctcaccact gagctaaagg gccgggtgca ggataataac ggtacgtaac
      901 taatcctgca atatcatccg ttctgactgg ctaaatcctg aacttccctg accgtctgct
      961 caaaacgttc agtctccagc tcaacaccag ttgcacgacg ccccagtgcc atcgcggctt
     1021 tgactgtcga acccgacccc atgaaaaaat ctgcaaccag gtcccccgga cgactgctcg
     1081 cgctgattat ctgctgcagc atttctgccg gtttttcgca cggatgtttc ccgggatagt
     1141 actgcaccgg tttatgcgtc cacacatcgg tgtacggcac ctgcgccgtc acgccaaaat
     1201 accgccgcag atgcttatat tcactctgca gttccgcata ctgccggttc agtgaagtat
     1261 acgtctccag cagctggtgg tggggcttct ccagttcacc ccgctgatgc ttctcttctg
     1321 ccacccgggc aaacagcgcc tgtaatttca gataatcact ttcgttcggc agttgccact
     1381 gactggcact gaaccagtgc gacaccatgt ttttctttcc tgtggcatcc actatctgtt
     1441 ttgccgttat ccccagggca gcacgcgcat cacgaaagta agcaatcagc ggagccatca
     1501 catgctgttt cagtgccctg cccttcgcct catacccggc atctttcgga cgatacggcc
     1561 cctgataatg ttccgcgaac agaatgcgct ctgtggcggg gaaatacgcc ctcaggcttt
     1621 ccttgttgca cccgttccag cgtccggacg gctttgccca gataatatgg ttcagcacac
     1681 tgaagcgttc acgcatcatg atttcgatat cagatgccag gcgatggcca cagaacaggt
     1741 aaagacttcc ggcaggtttc agcacccgcc agaactgcgc aagacactgg tccagccact
     1801 tcaggtaatc atcgtcgccc ttccactggt tatcccagcc ctcaggcttc actttaaagt
     1861 acggcggatc cgtgactatc aggtcaacag aattttcggg taacgaccgg ataaattcca
     1921 ggcagtcggc gttgattaac tcacaactgg atatttttac agtattaagc atggatcatt
     1981 aagcctgtct ctgataggct cattctgctt ttgcgcaaag cagtgggcct gaggtttgct
     2041 tgtgatccgg acgcatgagc agatggctgg tgagtgcccc taacacccac cagccgccca
     2101 tttaccacaa ataaaaaagc cttcaggact gaaggcgtct gtaacaaccg aactgatagt
     2161 ctgccagacc cgccataaca agctgggtca gtattaactg acagcgttcg cgtgaaaggt
     2221 acgtattctg cgcaatctcc ccgactgtcg ccggttcggt gacgcttaat tcattaaaca
     2281 ccactctggc ggtttcggtc atatcctgct gtttcagcat gtctttttcc cttttccggt
     2341 taacgtgaca caccaataac tcttgtcgaa aaagccagca agctgaaaga ccggtattca
     2401 ccgccgccag cgcgtttact gtactggagt gatttcagtc ataaaaaaac ccgcctggcg
     2461 acgggtgtaa aaagtcttct aacgtcaggc ataaaacgcc catcgttagg gcaaatttac
     2521 cacagattcc ggaaaaatca accttgttac ctggttacct tttttaactg cctctcagcc
     2581 catgcttcct caatatcaaa cttgctcacc agcgcatcat agaatttctt aactgttttt
     2641 tcccatgacg cgcgtgttat ctggtttgtc acctcgcata tagcattaaa tgcctctgtt
     2701 gacggcaatc tttcatagcc acgaccacca caacgctggc agtccctgat aacaggcata
     2761 ccccgttttt cagactcttc acgatgaatg gcaacaccgc gcccacgaca atctttacag
     2821 gcggtggaaa cctccccctt tccgccacac tccggacagg caacttttac cacctccctg
     2881 acttttttcc attcctccca gtaagacgga tacacgcctt ttgtgcactt tgcccacact
     2941 ggcggcttac tatccggata ctggatcttg tttgtaaaaa cctcgctttc aataaatttt
     3001 tttccatgac aacaggagca ctgttttttg ctcgccgcgc tacgggcata atcttcaaac
     3061 gcatacgaag ccataatacg catcactgcc ggttttattt ctgccgggag ttttcttaac
     3121 gccgccacgc aatcacaccg actgagtgca tattctgtca gcaattctgt tgcccgttct
     3181 ctgtcattca tactaatgcc cattttccca agaaacgcag aaaaccccat ctcagcccga
     3241 ttctgtgtca tgccctgcgc ggccatcaca tcagtgatac tcagcgcatc tttcgacgtt
     3301 gaggccgatg catcagtcag gccgggggat tttggggagt agtatttcgg taaatcttcc
     3361 agtttcattt tttgacctgc ccttcaagca ttatggggta aatcttcacc cccagccgtc
     3421 caccagatac tggcttgcca cgtacaatat tgatttcatc aaactgctcg tcatcaataa
     3481 gcactcccgc atgcgtcagc gcatccagcg gtgctttcag aatattgtcc aggtcacgac
     3541 gacgcttatc cggtggctct gcaataattt ttattgccag ccgtccggac aggcttaatt
     3601 tcagccgctg ctggcgaaca ataagcgcca ctgcccggag atagcgcttt ccctcctccg
     3661 atataaaata tgtgctgcca cggcgtcgcc agtaagtgtt caccgtcggc gggtaaggca
     3721 aaacaaattc tatgcgctcg gtcatttatg ctttccactt cagaacaccc gaatttctcg
     3781 cgtgcattaa aaaacgaatc agcaacaaca gctgactgcc gtgtttctct tcaaaatctt
     3841 ttaccccggc gtgtagttcg ttatggcatt tacggcataa cggaataaca aacaaatcgt
     3901 cagcctttgt tcccatacct cccagtccat gaccaatgat gtgatgcgga tcatctgcct
     3961 gattgccaca cgtcatgcat ttctgcgttt ttacccagcg cgtgtataca ggcatctctt
     4021 cccgttgtgg tttctggcgc tggaggtact gagccggaga ctccggatca acggcgatgc
     4081 ttactaacgt cttttcctgg gggggatttt gttgctggtg gacgtgaagt ggcagcgcaa
     4141 tattttttgt gcgctgcttc agtatgctgg tggcggcctg ctctcccggt atgatgtcgc
     4201 tctcacggta tacggagcgg attttttccg ctggtaatcc cagcgaacga cgcactactg
     4261 cctcaggtag tgcatccacc acctgattgc agaccgccca ccaggataat tcggccagcg
     4321 ataactccct ctcctgcgta ctgcttattg cgtgacggat gacgtcaatc atccaggcaa
     4381 ccagattctg ctgagcaagt tgatcgagtg attctgatgt ctggtcgcgc agctggttgt
     4441 cacagtgcca gcacaacacc atcgcgccgg taccgtaacg gtgaatgacg gtttcgctgt
     4501 gatgataatc accgtgtggc cactggcagg atttcacgtg acgtaataac cagtcagaca
     4561 gtgcacctgc accacctgct gcacgaataa cccgctcatc gctgaaaaat ggcagtaatg
     4621 ttttatcctc tgccagcggc tggcgaacgg caggaacgac tccggacggc agaccgcgca
     4681 tgtttttcgg ttccggttcc accaatattc tgccgttatg gaatgctgac attgattcac
     4741 ggcccggctt aaggagcacc atcccgagtt ccggtaccag aacaggtcga agtaataccc
     4801 gcacgttacc tccagatgcg ctgctggaat gtgcgggacg gacgcggtgg gcgttcggaa
     4861 taagggagcc tgacggagat tatccagtga cgacgatcga agctgagatc tttctgaaac
     4921 tcgtaaccac gtctgcggta gcactggatc agccattcgg cctgttcttc agtgcatggg
     4981 tcatgctgga accagtcaga tttgaatgca tgagaacgcc gcccgtgcct gctggcaggg
     5041 gcggtggagt tatccgaatt gtgtaatttg gtatcttgcg ccatcggttg tctctgctgg
     5101 cgcagcaggt gccagttgtt caggctggcg tgcggcaata ttgtctctga tttctgttgt
     5161 cgtcaacagg cagcgtgcta tcatcgaata gtgttctatc ctactccgtg aggtttacca
     5221 tgcgtacaac ccaacaattc agcattacat taactaacga gatggctgac atggtgcgcg
     5281 cccgtgtggc ttccggcgcc tatgcttcag aaagcgaggt cattcgtgaa gggcttcgcg
     5341 cactgaatga gcgcgataaa gcaatcgaag cgtggttaac gcattcagcc gccccctctc
     5401 ttgattctat ccgcgaaaac ccaaacaacg gacgctccat ttcacaggtt cgcgtcgcga
     5461 ttcgatccgg gaagtaatct gcatgacata tgaagtcatc attactcctg aggccgaaca
     5521 acaaataatc aacctgcaca gatatataac ggagaaagca gggaacatca ttgctgacaa
     5581 ttatgccaat gcgcttcttg attatcttga tgggttttct acattcccgc atcggggcaa
     5641 taaacgcgat gatattcgcc aggggatgcg ggtaactcat ttccgccaca gaacgattat
     5701 tgcttttgcc gttgatggca gaaaagtctt tattgtcggt atctatcatg gtgggcaaag
     5761 ttatgaaacc gatttcttat aaacttttac ccacatcatt ccggtgttag aataaaccgt
     5821 ctgcccctct cttactgcgg attcgtaggc tatataaatc aaagatcccg gctcatgttt
     5881 gtggcgggat cttttttcgg cgatttatcc ccagcggcaa atcgaataca ccaccagcgc
     5941 caccgccatt gcaataccaa catttgagaa ggcctcaggc cagctcattg gcgcacctcc
     6001 tgcggcggtt ctggtagcgg catccagtgt gatggtatcc acgacgcacc aggtactacc
     6061 cacccatcat tagcgtcagg atgccccggg atgtaagtcg cccatttcat tcgccagtca
     6121 cctttcctgt caaactccct ggcaacaaga acggctgttt tggtatccgg cattcgctca
     6181 ctacagctta tccaaccatc cggagttacc ggcactggct tggcggtata aagcggtgtt
     6241 atatctgccc gaaaattaca tgctttatgc agccgcaccc accgttcgac ttctgctttg
     6301 tcagaataca taccagtgaa cgtgttatat tcacggtcaa tttgcgtgaa tgttaccttc
     6361 cacgccaccg gctctgcttc cagagatgcc agtgcgatac gcagggaagc caggatattc
     6421 ccctggtaat catcaagccc gaaaggaagc tcatcacgaa cgctttcata atcgttgata
     6481 gcctgctgca gccattctct tgtgatagtg ctcatgattc ctctccttta ccggctgcgg
     6541 cggcgatacg aaaaagttta atgtcgccgc tgtctgagtt ctgcttactc cagataagtg
     6601 acgtttctcg cccgcgctcg ttgatatgca tgagatttct ttcgtctgta aagcatgcag
     6661 ggatgaaagc tacagggagt ggatagaggg caactgcatc atcaccccat tgctcaactg
     6721 catatttctc atcggttgtg aacctcgaca tgaatgatgt gacgttagca ttcctcatcc
     6781 atgcaactgg cttaacgtcc cgcgcctgca gctcagcaat ccgcttctct gctgcttcca
     6841 gctcaacacg cagcttccct accgttagcg caatatcctc gttctcctgg tcgcggcgtt
     6901 tgatgtattg ctggtttctt tcccgttcat ccagcagcgc cagcacggta gctggattgg
     6961 ctgcggcgat gaattcagca ttggcctgct gttccatttg gaaatcttca tcgaaaccgc
     7021 tttcaggatg cgctccttca attctgcaaa tgggaatata tccagcaact tcacgatgaa
     7081 ttagcgcatc atcaccatca aatcgtccct ctccatattc gagcgaccac tcgccacacg
     7141 ttgctttttc tgccttagca cgcaatgcct gatagtcaat cttgctcact ggttgcctcc
     7201 tttgcgaagc tcagcggcga aggctactgc gtgatcatga tgttcaagtg tgtatgcaca
     7261 ctccgcaaac atctccacgc cctgcgctcg tacttcagcc aggaaagcat cggtggcagg
     7321 catatttcct gttgccttca tggcctccaa aataaccaga acgccatctc tcccaacctc
     7381 ctcgcagata acctcggtgt tgtcgccaac aacatcgcag aatgcctgaa ctgctttacg
     7441 agcaagtgca ttctccgccg ccagcgccac aagattagct tccagctctg caatacgttt
     7501 gctttgggct tcccgttcat ccagcagtgc ctgtactact tcagggttga aagctgcaat
     7561 aaattctgcg ttgttctctg catttttctg gtcatcaaag ccaggccatt tgataatgtc
     7621 tccgcaacgt tcatcccccg gcgtatgcac cgcatatgta tcagtgcccg gcgaaatgaa
     7681 tgcgacccat tcgccctggg ttgccagttt tgctatctca cgtagcgtct gataattaat
     7741 ttttctcact ggttgcctcc tggaaaataa ccgcatgtcc cagtttctcc gccagtgcca
     7801 gttctgcctt agcgcctgcc gaccgctgcc agcctttcag catgtaaatc gcatccacgc
     7861 agcgtatcat cgccatgcaa atatccatgt agtgtggctg tgtcagtccg tccggaagta
     7921 ctgccgggtt taaaacggta tgcccttccc gtttcagtgc ttcttccgcc ctgtgaaacg
     7981 cctcacggtt gaaattttca tatcccgtca tcggaccggc gatataaatt ctcaccctca
     8041 cgcctgaacc ctcctgtcga aataaacgta gttattcact gcgcccaact tcatcccaaa
     8101 cttttcggca atttcccgtc ggggtacgcc acgctgatgc agttgccgcg ccagctcaat
     8161 atcacgctgt gaacatttgg ctgactggtg ataatcaccc cgtaacatca tgcagatacc
     8221 cagttcccgc gctttcgtcc tgacggcagc cccactacgg ccaatcagac tgccgatgct
     8281 ttcgactgtc atcgttcccg cacactgccg gagtatcata atttccgccc agcgccactt
     8341 cttccagcca ctcaccgcag cagctctctg gtggcggtaa tatcccggag aatatccctg
     8401 tgtttgttca gttcccgcag cgcggcacag actcgctccc acttctgaac atcacttttc
     8461 gccctgcgca gcgccaggtt tgccctgcac agggacagaa aaatcagctc atctgcttgc
     8521 gtttcggtaa acgatggcaa cgactgcaca atgtccgcca ctgtttctgt tttaatatct
     8581 tcctgtgttg cagattcctg tactggtaac gcaacacctg ctggctgagg aaaggcctta
     8641 ccatcatttt ccgttaccgg cacggctttc ggctctgctg gtaaattatc gcccggcatg
     8701 cagtaacgaa atttaccgtt ctgatttacg cgtgccaggc gtcccgttgc ggttaccacc
     8761 gccaacgtgg aagcaacctt gcgaatgcta acaccgaact tatccgccag ttcctcacac
     8821 gttttagccc cctcctgacc gataaactca atcatcatgt ctgcggtaac tttttgttcg
     8881 acctccccgg tcagcacatc cggtacttca gactgttctg gctgctcttc ggttaccccg
     8941 gattcacctt cgccagccag aaaccagatg tgacccgctt tatcaacaac gccatttctt
     9001 ttgagttccc acagttcgtt gagaacctct tcacgactga tatcaagccg ctcagccagt
     9061 ccaacagagc tggcttttcc cattgctttc agtgcgtcaa aaacggtttc cattaaaatt
     9121 tcctcctaca aaatcgcttc tcagattcaa ataaaaccag ctgccttccg gcgttcgtat
     9181 tcctgtttca gccattcaat tggcgttggc cctttcgggt gtttcgcccc ttccagttgt
     9241 cgtcgcactg gcggaacact catcccgcta ccaacatgct ttgcccattt cgtcagttgc
     9301 cgttccgcaa gtcgttttaa ctcaccctgc gtcatctggc gctcaatccc tctggtacgc
     9361 atttcgaggc agatgtggta cagcacaggc tgtggccacg ggtatttatc actcccgtcg
     9421 tatcgccagg attcattgcg ccagcgccgg tactcttcca tcacggcatc caccgtaaga
     9481 ccaaatggat ttgccccact ctccgaaatc agcgcaacaa actcagcaag gtccgggggc
     9541 cacgtttcac ccgcccggca gcggtccatg cactgacgac agacctgccg gatttgctgt
     9601 tcagtcatcg caccaatctg ggcaatccag agcttcgaag gtgcggcccc gttcttctga
     9661 gtccagcggt tcgaatacac ctcccccata agctcccaca gcttccaggc cgtttccgtt
     9721 gctgataaat ccgttgtctc gttcccactg ttcgcgtgct gcccggattt cctgaattgc
     9781 ccgtgatgcc gtgccacctg atgctgcatg gcttaccccc ttgctgactg gttttacctg
     9841 tgccctgacg tgctgcacgt ggcgggcaaa tttctgctcc cactgaacct gcgtgaaaac
     9901 cttcccctcc gccatccagt aatcccggaa tgcggcaagc tctgcaggtg taaattccgg
     9961 ctcaggcaga gccatacccc acactgctgc ccgttgtcga aaatccggcg acggctgcca
    10021 gacagtggtc atcgaaaatt tcccgatcgg ttcgctcagg ccgtccaggt attcaggttc
    10081 ggctgtctgc aacgacgcac cattcgactc accggttgga gcactctcgc gcatgcgcgc
    10141 gttatgtgtg gggtttaatt ctgtatctgt atctttatct gtcgtgactt gtcgtgacag
    10201 atgcgtgaca cgtcgtgact catcgtgaca atcagcatca tatttccgca gcttttcgcg
    10261 ctcccgctgc gctctcttgc gctctgccgg ggattttgcc gtttgcgaaa cgttaccatt
    10321 gtcctctttc agcacctgac gtttttccca tccggaaata aggtcaccat ccaaaacccg
    10381 cccctgcatt gcatgcaaaa ttgaatcaat tacgtcttcc gtcacatcaa gcgcacttgc
    10441 taaatcttcc gtcgtgacat caatgtgacc acgtagtgac acgccgtgac atgtcgtgac
    10501 atttcgtgac gcgctcacca gaaggtggat atacactgcc atcactgttg cgattggctg
    10561 tcctgatacc ctggcaatcg ttcgccactt ggggtcattt ggcatgtcat gccacaatct
    10621 gagccaggca ttagccatac tcacctcttc tgataccgaa ctttacccac gaacttccgg
    10681 aagaaatccg gtataaatat tgttggtcaa tgcacaacaa cagcattacc aggctgacca
    10741 ccactgttag tcagggtgcc ccaggcgatc gctacagcga caaaatcatc cacatctttc
    10801 accagccgat cccgtcgttc gacgatctcc cggtaatatt cagaactgtg actgcgcata
    10861 cgggccacca gcaaaggcgg catcgccttt tcgatcgccg gtaacagcgc ctgaattttt
    10921 tcaacagcat cagacgtgtc cttctccacc cagcggaaaa ttttctgggt attacgagcc
    10981 agggcttccg gatggctgtc gtcgtacagt tccgggaacg tcatccccag ctcgaaataa
    11041 gtccgggcta tttcagctgc gggaactttc tcaccatcag gatatgccca ggcattcatc
    11101 gccatacgga tgtgttcatg cttgattttc atgaatcacc cccgtctctg gttgtgtgtt
    11161 agcctgacac tcgacaggta aaccgtcggt cggattaggg taaatgtctg ggcgaatttc
    11221 atgcggggta acctcccact tcattagctg acatagcgga attacctgct ttgggggaac
    11281 gccaaagcta aaccattgcc aaactgtctg ttgagcgacc cccatataac gacctatttc
    11341 agcctgagtg tatttctgcc taattttttt gcgagtgcta tctagcattt tgccctcctc
    11401 taaaaactat aggcaaaggc tacaataaaa aactgtacgc gatcaacagt tttttattgt
    11461 gatgctttta acagtattta cctgtaaaat tgaataatga tgagcgccct agaagtatcg
    11521 atgtacagaa tcagcaagct tcttcaggaa actggatgga gccaggctga gctggcccgt
    11581 agaattggtg tgacacaaca aactgttcaa caatgggtca gcggtaaggc tacacctaaa
    11641 gcctcaagtt tggataaact ggttgaggtt acagggcatc cgttgcattg gtttttattg
    11701 cctcctgaag agggggagca aatgttcacc cctaacacga tgaaaattgg tcctcgccaa
    11761 cgcgaattac tccaggcttt tagtgcgttc cctgaggagg accaagaaaa aatgcttcaa
    11821 gaaatcaaag acaaaaaaaa atcaatggaa gaaaccattg cccggtggct ggcggcacaa
    11881 aaaagccgcc gggcgtgacc acagtacaag aagaggagtt atgccatgag tacagccctt
    11941 tctccgatag tttcagaatt cgaaactatc gaacaagaaa acagttacaa cgaatggttg
    12001 cgcgctaaag tagcgtcaag ccttgcagac cctcgccccg caattccaca tgacgaggta
    12061 atagctgaaa tggaaaatct tattgctcaa attgctgtaa ctaacaggag cgagtaatgt
    12121 tgcccatttt atggctacca tctgctcgcg atgatttgcg tcagatcata acttacatcg
    12181 ccaaggagaa cccaccggca gcgcgtagac ttaaaatacg tattgaaaca tcggtattac
    12241 ctctatctga gcatccgtac ctatatccac caagcgaacg ggtttctggg ttgagagaga
    12301 tagtgaccca ccctaactac ataatcctgt acagagtagc tgcttcaagc attgagattg
    12361 taagcgtgac acatgctcgc cgacaatttc ccttctctat ctgagctgaa ctattttcat
    12421 actccctctt tcgagggatt tttttgctta agggcacaat taaaaactgt tgacacaaaa
    12481 cagttttaaa ttgtagattg cactcaccaa cccactccgc cccacagaac gccaggcaat
    12541 acttcgagtt acccggcagt ggtcaggggt taagtagcca gcccgaggcg tatgaacatg
    12601 acggcgggaa cactttgtat aacagcgcag caggttttta gttccgcgac ccggcgttaa
    12661 gggtaaatga ggtcaacatg gatacgctca atcttggcaa caacgaatct ctggtatgcg
    12721 gcgtgtttcc caaccaggac ggcacgttta ccgcgatgac gtataccaaa agtaaaaagt
    12781 ttaaaaccga agctggcgcg cgtcgctggt tagccagaaa cactaactga ttagcaccag
    12841 taaaaacagg ttgccactgg ttaatttacc ctgaaaagtc agggtataac acgaaagcgc
    12901 acggcgaagc tctttccctt agaaggcttg tcgttagatt tcttcgaacg tgcgcttccg
    12961 gttgtggcaa tccgcgaaat ggcgcggcgg taagtatggc tggggtttcc tccattgctc
    13021 cagaaaatgc accgggttgt caggttgacc atacgattaa gtgacagccc cgccacaata
    13081 cccatgtgta gtctttggtg gcatcagttc tactccgtga ctgctctgcc acccttttta
    13141 aagtgaattt tgtgatgcgg tgaatgcggc taagcgcacg cggaacagtt aaaacaagcg
    13201 gtcttttact ggcgtaacag acatcaacta acaatccggc gttaattgtt aactggttaa
    13261 cgtcacctgg aggcaccagg cactgcatca acaaagttca cttcggtgat gaagggtaag
    13321 agaaaatgtt gaatgtagct attgaaaacc agaatgggtg gaattatagt gcacctgcac
    13381 ctcataaagc gggtgccggg cgtggaaacc cgatgatgac tactgcgcat aaccgcgctc
    13441 aggcggtttt tttatgcgta atgcacagcc acattcagat tatggtgggg cgtgcagggc
    13501 agccgaaagg ctggccggtt tcggtagtca ccggtatttc cacccctgta cgtctcacca
    13561 cccttatggt cgtggaaagc cttggtggtg agttatttaa actgactatc gaggctgcca
    13621 tcatggctac tatccctgcc ctttctcacc ctgacgtaac catcgaaaat ggacgcgctg
    13681 tcactacgtc tattgcgatc gctgagttct ttggcaaacg ccacgaacga gtgttggata
    13741 aaattcgcaa tctggactgt tcagcaaaat tcactgagca caattttgtg tcgagcgaat
    13801 ataccgactc aaccagtcgc aaactcccaa tgtaccaaat caccaaaaac ggcttcgttt
    13861 tcctggtgat gggcttcacc ggcaaaaaag ccgctgcatt taaagaagcc tacatcgctg
    13921 agttcgatcg catggagaaa gaactgcgcc agaataacgc cccgtctccc gacaaaatga
    13981 ttcacgggga cggacgtacc ctggttatcc gtctcgacga acacggcaat atcaaattca
    14041 ctgaaaccgt tccggacggc gcaatggtct gcaccctgga taccttccag ttttatctgg
    14101 agaaacaagg ctggactctt gtaaaccgga gcgcaattaa aaatatgact gtggagcaat
    14161 tactaaaaat tcattgttga ggacgcgata atggaaacgt tattaccaaa cgttaatacg
    14221 tctgaagggt gttttgatat tggtgttctg ctcagtaacc gggagtttac tgaagatgcc
    14281 attaagatga gaaaatatga gccttatctt ctcaatgata attccatact ctccagaatt
    14341 gcccttctta aacttggtat tttcggaggg cagcagtgac ttctgcattt gcactggtga
    14401 tgacggtttt tcttataacg ggtgagccac agaatgtgat taccggaatt tatgccagta
    14461 aagaatcatg cctccgtgca agagacgagc aaaaaatttc tggcgaatgc ctcccgttaa
    14521 aaaaagtatc gctgtacctg aataacgaaa taccggctgg ataatccacc agccatatta
    14581 acgccatacc cgttgattaa gcatgccagc aatggcaggg gttcgtacaa ccttaaaata
    14641 gttatgaggt ttatccatga gcactgataa agaagaattt tcgctatatt gcgaagcaaa
    14701 aaatgacaag gtcagaaaac gccttgggat taaaggaggt ttttactgga ctacagcaaa
    14761 aaaattatct gttgccatct cacggtgcgt tgttgcaatg gacgaggcag gctacgacgc
    14821 tgatgatttc aaaaaacctg tccgcgtcca tttccccgtt gtgaatgacc ttccaccgga
    14881 aggcgtgttt gataccgaat tctgcaaccg ctatgaaaaa ggcggggaag atggcatcac
    14941 aatgatattt atagcgcctt ccccctcagt tcaggacaaa ccagccagca ctgacaatac
    15001 caacgtcaat ggcgaagaca tggctgagat tgaggataat atgctcctgc cgatttccgg
    15061 tcaggaactg cccattcgct ggcttgcgca acatggcagc gaaaaaccgg taacgcacgt
    15121 ttcacgggaa gaacttcagg cattacatat cgcacgagct gaagaactgc cggctgttac
    15181 tgccctggct atttcccaca acacaaagct gctcgacccg ctggagattc gcgaccttca
    15241 caaactggta cgcgacacag acaaagtttt ccctaatccc gttaattcca gtctggggtt
    15301 aatgactgct tttttcgaag catacctgga cgctgactat accgatcgag gtctgctgac
    15361 aaaagagtgg atgaaaggaa atcgtgtttt acgcatcagc cgcacgccat ccggcgctaa
    15421 tgctggcgga ggaattctta ccgatcgcgg tgaaggtttt gtccacgatg atgcgtcagt
    15481 ggaacgtgac gttgccgctg gcgttctggc ccgttcaatg gacatcgata tttacaatcc
    15541 acatccggca cacgccaaac gcattgaaga aatcgtttca gagaataagc cgcccttttc
    15601 tgtttttcgt gacaaattca tcgccatgcc tggtcacctg gattattccc gcgcgatagt
    15661 ggttgcgtcc gtgaaagaag caccaattgg tatcgaggct actccccacc gtgttaccga
    15721 atatctgaac aaagtactga ccgaaaccga ccatgccaac cctgatccag aaatcgtgga
    15781 tattgcctgc ggtcgctcct ctgctccaat gccgcagcgt gtaacaaaag aaggaaaaca
    15841 ggatgatgaa gaaaaaccgc agccatctgg cgcaatggca gatgaacagg caacgactga
    15901 agcagtggaa ccggatacaa ctgaacataa tcaggacacg cagtcgatgg atgctcagcc
    15961 acagataaat tctgttgatg cgaaatatca gaaactgcgt gcagaactct atgaagccag
    16021 gaaaaacatt ccacccaaaa atcctgtcga tgcagataaa ttactggctg cttctcacgg
    16081 agaatttgtt gaagggatta gcgacccgaa tgatccaaaa tgggtgaagg ggattgaaac
    16141 ccgcgattct gtgaaccata accagcaaga aacggaacag aaaggccata aagcggaaca
    16201 acacagtcca aatgcgcaac aaaacgagcc agaaacgaaa cagcctgaac cagtagcgca
    16261 acaggaaccg gaaaaagtct gcacagcctg cggtcagacc ggcggcggca actgtcctga
    16321 ctgtggcgcg gtgatgggcg acgcaacata ccaggagaca ttcaacgaag aaagccagga
    16381 tgaagcccgg gaaaaagatc cggaggaaat ggaaagtgcc ggactcccga acaaggagtg
    16441 caccgaaggc gatcaacatg ccaatggcaa taatgaaaca ggcgagacag caaatccctt
    16501 aattaaggtg aacggtcatc gtgaaatcac atccaccagc aggttgtggc accatctgat
    16561 gattgacctt gaaacaatgg gcaaaaatcc tgatgcgcca atagcctcaa taggcgctgt
    16621 atttttcgat ccacaaaccg gagagcaggg gcctgaattc agcaaaataa ttgatatggg
    16681 tacatgtggc ggcactgtag acataagcac catcgaatgg tggcttcaac gctctggcga
    16741 agcccgtgcc gccattttag ctgatcgaat accgcttgat gatgcgcttt tacaattacg
    16801 ggaatttata gacgaaaact ccggtgagtt ttttgttcag gtctggggaa atggagccaa
    16861 cttcgacaac gtgattttac gccgttcata tgaacggcag gagatcccct gcccgtggcg
    16921 ttacaccaat gatcgcgatg taagaacgat ggtcgctctg gggttggtga tggatttcga
    16981 tgcccgcaac gtcaccacat ttgagggtga acgccataat gccctgcacg atgcgcgtta
    17041 ccaggcaaaa tacgtttcag ctatctggca aaaactgttc ccaaatcagg ctgattttta
    17101 atgttcaacc ctgatcgccg ctaaccgcat atagttagcg gcggttatga gatatagcta
    17161 tgagcagctt atttttaacc gaagatgaat tgctaatatt aacgggctgc aaatatgcaa
    17221 gccaccagcg caactggtta ataaaaaatg ggcttccgtt ctataccaat cgtagtggaa
    17281 aaccgatcgt cagtcgggaa ctgtttacct gtagaaacac tttaccacca cgtgaggctg
    17341 aacctgattt cggtgcgatc taatgggaag acgaaggaaa aatcccgaac acgaaaaatt
    17401 accgccaaag gtatatccca ataaatatag ttatgtatgg aaaccaacat ccagagaatc
    17461 tgtaacctta actgcaatca aggatggttt agccgcatta tggaaaaaat atgaagaaac
    17521 ggttaaccat catgatcacg caatgacatt tgggcgtttg tgggaaaaat ttctcgccag
    17581 cgcctattac agcgatctta gtcccagaac gcaaaaagat tatctgcaac atcaaaaaaa
    17641 actgctggcc gtattcggta aggtgctggc cgattctgta aaaccagagc acatcagacg
    17701 atacatggac aaaaggggcg agcagagtaa aacgcaggca aaccatgaaa aaagcagcat
    17761 gtcacgcgtt tacagttggg ggtatgaacg agggtatgta aaggctaatc cttgtgccgg
    17821 tgtaagtaaa ttcaaggcca aaaaccgaga gcgctatgtg actgacaaag aataccaggc
    17881 cgtattaagc gtggctcctg tgcctgtttt tatcgcaatg gaaattgcat acctgtgcgc
    17941 agcaaggatt tccgatgtgt tgtctctgaa atgggaacag atcggaaacg acgggatctt
    18001 tatccagcaa ggtaaaacag gaaaaaaaca gataaaagcc tggactcccc gtctgcaatc
    18061 agttatagaa aaagctaaac agctacccaa gtcagcctat gtaatcagca atcagtacgg
    18121 caaccgttac atgtataaag gatttaatga aatgtgggtt gaggcaagaa atttggctgg
    18181 acaaatatca gggatcgcga cggattttac attccatgac cttaaagcca aggggatatc
    18241 agattacgaa ggtagtagcc gggataagca acttttctct ggtcacaaga ccgaaggaca
    18301 ggtgctaatc tatgatagaa aagtaaagat ctcacccaca ctggatgtac cattacccca
    18361 gaatattcca acaaaatatt ccaagtaatt ccaagtgtga ttttcatcat tgacttaatg
    18421 atgtgtaagt gattgaattc tggcggagag agggggattt gaacccccgg tggagttgcc
    18481 cccactccgg ttttcgagac cggtccgttc agccgctccg gcatctctcc gttcagatgg
    18541 ttgccatgat gccaggaaat ttggcatttt aacagtccct gtccgtgcaa ttttgttcaa
    18601 gtgacgagtt tgcgagcaaa acgatgatta agtggccctg gaaagtacaa gaatcagcac
    18661 atcaaactgc ccttccctgg caggaagcac tatcgatccc ccttttaacg tgtctgacgg
    18721 aacaggaaca aagcaaatta gtcactcttg ccgaacgttt tttacagcaa aagcggcttg
    18781 ttcctttaca gggctttgaa ctggattcat taagaagctg ccggatagca cttctatttt
    18841 gcctacccgt tctggagtta ggactggaat ggctggatgg ttttcatgaa gtcttaattt
    18901 atcctgcgcc atttgtggtc gatgatgaat gggaagacga tatcggtctg gtgcataacc
    18961 aacgtattgt tcagtcaggt cagagctggc agcaagggcc tatcgttttg aactggttgg
    19021 atatacaaga ttcttttgat gcttctggtt ttaacctgat tattcatgaa gtcgctcata
    19081 agctggacac ccgtaacggc gatcgcgcca gcggagttcc ctttattccg ttgcgtgagg
    19141 ttgctggctg ggaacacgat cttcatgctg caatgaacaa cattcaggaa gaaatcgaat
    19201 tggttggcga gaatgcggcg agcattgatg cttatgctgc cagtgatcct gctgaatgtt
    19261 ttgccgtact ttctgaatat ttctttagcg ccccagaact ttttgctcct cgtttccctt
    19321 cattgtggca acgtttctgc caattttatc aacaagatcc tttgcagaga ctgcatcgca
    19381 ctaatgatac agactcgttt tcggcgacga atgttcatta attaacaact ttgcagatta
    19441 attaaccaat tgaaatgact tatgaaattt agtgttgaca gacaaggtac cgctaagtaa
    19501 tattcgcccc gttcacacga ttcctctgta gttcagtcgg tagaacggcg gactgttaat
    19561 ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tctaaaaatt cgcttcttag
    19621 cgcaatgtca ctaaccttag ttgaacattg tttattaacg gatagcgggt ttttaacatc
    19681 ttaagcgccc tcgaccttta tggttgaggg cgttttgcta tgaacgccat caccattttc
    19741 ccctcgatta taaaacttga gttattcagt agtctcccct cttgcaactc acacccaaaa
    19801 ctgcctaacg aaaagttatt aattttcaat catattgcta tcaggattta cattttttcg
    19861 ctgtgctaga aagggcgcat ttatgttagc tcgttcaggg aaggtaagca tggctacgaa
    19921 gaagagaagt ggagaagaaa taaatgaccg acaaatctta tgcgggatgg gaattaaact
    19981 acgccgctta actgcgggta tctgtctgat aactcaactt gcgttcccta tggctgcggc
    20041 agcacaaggt gtggtaaacg ccgcaaccca acaaccagtt cctgcaccgt attgccattg
    20101 caaatgccaa tacggtgccc tacacccttg gagcgctgga atcggcccaa agcgttgccg
    20161 aacgtttcgg tatttcggtg gctgagttac gcaaactcaa ccaatttcgt acgtttgctc
    20221 gaggttttga taatgtccgc cagggtgatg aactggatgt cccggcacaa gttagtgaaa
    20281 ataatttaac cccgccaccg ggtaatagca gtggcaacct tgagcaacag atagccagta
    20341 cttcacagca aatcgggtct ttgctcgccg aggatatgaa cagcgaacaa gcggcaaata
    20401 tggcgcgtgg atgggcctct tctcaggctt caggcgcaat gacagactgg ttaagccgct
    20461 tcggtaccgc aagaatcacg ctgggcgtgg atgaagattt tagcctgaag aactcccagt
    20521 tcgattttct ccatccgtgg tatgaaacgc ctgataatct ctttttcagt cagcatactc
    20581 tccatcgtac tgacgagcgt acgcagatta acaacggctt gggttggcgt catttcactc
    20641 ccacatggat gtcgggcatc aacttctttt tcgaccacga tcttagccgt taccactccc
    20701 gcgccggcat tagcgcggag tactggcgcg actatctaaa attaagcagt aacggctatt
    20761 tgcgactgac caactggcgc agcgcacctg agctggacaa cgattatgaa gcccgcccgg
    20821 ccaatggctg ggatgtacgc gcagaaggct ggttacccgc ctggccgcac cttggcggta
    20881 aactggtcta tgaacagtat tatggcgatg aagtggccct gttcgataaa gacgatcggc
    20941 aaagtaatcc tcatgccata accgctggac ttaactatac ccccttcccg ctgatgacct
    21001 tcagcgcgga gcaacgccag ggtaaacagg gcgaaaatga cacccgtttt gccgtcgatt
    21061 ttacctggct acctggcagc gcaatgcaga aacagcttga cccgaatgaa gtcgctgcac
    21121 ggcgtagcct tgcaggcagc cgttatgatc tggtggatcg caacaacaat atcgttctgg
    21181 aatatcgcaa aaaagaactg gttcgcctga ccctgacaga ccccgtgaca gggaagtcag
    21241 gagaagtgaa atcactggtt tcgtcgctac aaaccaaata tgccctgaaa ggctataacg
    21301 tcgaagccac cgctctggaa gctgccggtg gtaaagtggt tacaacgggt aaagatattc
    21361 tggttaccct gccggcgtac cggttcacca gtacgccaga aaccgataac acctggccga
    21421 ttgaagtcac cgctgaagat gtcaaaggca atttgtcgaa tcgtgaacag agcatggtgg
    21481 tcgttcaggc acctacgcta agccagaaag attcctcagt atcattaagt acccaaacat
    21541 tgaacgcgga ttcccattca accgccacac taacttttat tgcgcatgat gcagcaggta
    21601 atcctgttat cgggctggtg ctttcgacgc gtcacgaagg tgttcaggac atcaccctgt
    21661 cggactggaa agataatggt gacggaagct atacccaagt cctgaccaca ggagcgatgt
    21721 ctggcacgct gacgcttatg ccacagctga acggtgtgga tgcggctaaa gcccccgccg
    21781 tggtgaatat catttctgtt tcgtcatccc gaactcactc gtcaattaag attgataagg
    21841 accgttatct ctccggcaat cctatcgagg tgacggtaga actgagagat gaaaatgaca
    21901 gacctgttaa ggagcaaaaa cagcaactga atacc
//
LOCUS       DAZGAN020000077        21632 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGAN020000077 DAZGAN020000000
VERSION     DAZGAN020000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.077"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            139..663
                     /locus_tag="ACHKYO_RS16170"
                     /old_locus_tag="pgaptmp_003234"
     CDS             139..663
                     /locus_tag="ACHKYO_RS16170"
                     /old_locus_tag="pgaptmp_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HGU4430029.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            747..1433
                     /gene="lpfB"
                     /locus_tag="ACHKYO_RS16175"
                     /old_locus_tag="pgaptmp_003235"
     CDS             747..1433
                     /gene="lpfB"
                     /locus_tag="ACHKYO_RS16175"
                     /old_locus_tag="pgaptmp_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HGU4430030.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQCQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1457..3988
                     /gene="lpfC"
                     /locus_tag="ACHKYO_RS16180"
                     /old_locus_tag="pgaptmp_003236"
     CDS             1457..3988
                     /gene="lpfC"
                     /locus_tag="ACHKYO_RS16180"
                     /old_locus_tag="pgaptmp_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HGU4430031.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLLSGLNIG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3999..5054
                     /gene="lpfD"
                     /locus_tag="ACHKYO_RS16185"
                     /old_locus_tag="pgaptmp_003237"
     CDS             3999..5054
                     /gene="lpfD"
                     /locus_tag="ACHKYO_RS16185"
                     /old_locus_tag="pgaptmp_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HGU4430032.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            5060..5584
                     /gene="lpfE"
                     /locus_tag="ACHKYO_RS16190"
                     /old_locus_tag="pgaptmp_003238"
     CDS             5060..5584
                     /gene="lpfE"
                     /locus_tag="ACHKYO_RS16190"
                     /old_locus_tag="pgaptmp_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HGU4430033.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5837..7528
                     /gene="eptB"
                     /locus_tag="ACHKYO_RS16195"
                     /old_locus_tag="pgaptmp_003239"
     CDS             5837..7528
                     /gene="eptB"
                     /locus_tag="ACHKYO_RS16195"
                     /old_locus_tag="pgaptmp_003239"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HGU4430034.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            7620..7696
                     /locus_tag="ACHKYO_RS16200"
                     /old_locus_tag="pgaptmp_003240"
     tRNA            7620..7696
                     /locus_tag="ACHKYO_RS16200"
                     /old_locus_tag="pgaptmp_003240"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7654..7656,aa:Pro,seq:cgg)
     gene            7731..7864
                     /locus_tag="ACHKYO_RS16205"
                     /old_locus_tag="pgaptmp_003241"
     ncRNA           7731..7864
                     /ncRNA_class="other"
                     /locus_tag="ACHKYO_RS16205"
                     /old_locus_tag="pgaptmp_003241"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8609..10216
                     /gene="dppA"
                     /locus_tag="ACHKYO_RS16210"
                     /old_locus_tag="pgaptmp_003242"
     CDS             8609..10216
                     /gene="dppA"
                     /locus_tag="ACHKYO_RS16210"
                     /old_locus_tag="pgaptmp_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HGU4430035.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10367..11386
                     /gene="dppB"
                     /locus_tag="ACHKYO_RS16215"
                     /old_locus_tag="pgaptmp_003243"
     CDS             10367..11386
                     /gene="dppB"
                     /locus_tag="ACHKYO_RS16215"
                     /old_locus_tag="pgaptmp_003243"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HGU4430036.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11396..12298
                     /gene="dppC"
                     /locus_tag="ACHKYO_RS16220"
                     /old_locus_tag="pgaptmp_003244"
     CDS             11396..12298
                     /gene="dppC"
                     /locus_tag="ACHKYO_RS16220"
                     /old_locus_tag="pgaptmp_003244"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HGU4430037.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12309..13292
                     /gene="dppD"
                     /locus_tag="ACHKYO_RS16225"
                     /old_locus_tag="pgaptmp_003245"
     CDS             12309..13292
                     /gene="dppD"
                     /locus_tag="ACHKYO_RS16225"
                     /old_locus_tag="pgaptmp_003245"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HGU4430038.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13289..14293
                     /gene="dppF"
                     /locus_tag="ACHKYO_RS16230"
                     /old_locus_tag="pgaptmp_003246"
     CDS             13289..14293
                     /gene="dppF"
                     /locus_tag="ACHKYO_RS16230"
                     /old_locus_tag="pgaptmp_003246"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HGU4430039.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14323..15594)
                     /locus_tag="ACHKYO_RS16235"
                     /old_locus_tag="pgaptmp_003247"
     CDS             complement(14323..15594)
                     /locus_tag="ACHKYO_RS16235"
                     /old_locus_tag="pgaptmp_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HGU4430040.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16070..16177
                     /locus_tag="ACHKYO_RS16240"
                     /old_locus_tag="pgaptmp_003248"
     CDS             16070..16177
                     /locus_tag="ACHKYO_RS16240"
                     /old_locus_tag="pgaptmp_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4430041.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16553..16660
                     /locus_tag="ACHKYO_RS16245"
                     /old_locus_tag="pgaptmp_003249"
     CDS             16553..16660
                     /locus_tag="ACHKYO_RS16245"
                     /old_locus_tag="pgaptmp_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4430042.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            17036..17143
                     /locus_tag="ACHKYO_RS16250"
                     /old_locus_tag="pgaptmp_003250"
     CDS             17036..17143
                     /locus_tag="ACHKYO_RS16250"
                     /old_locus_tag="pgaptmp_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4430043.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            17519..17626
                     /locus_tag="ACHKYO_RS16255"
                     /old_locus_tag="pgaptmp_003251"
     CDS             17519..17626
                     /locus_tag="ACHKYO_RS16255"
                     /old_locus_tag="pgaptmp_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4430044.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(17713..19392)
                     /gene="bcsG"
                     /locus_tag="ACHKYO_RS16260"
                     /old_locus_tag="pgaptmp_003252"
     CDS             complement(17713..19392)
                     /gene="bcsG"
                     /locus_tag="ACHKYO_RS16260"
                     /old_locus_tag="pgaptmp_003252"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HGU4430045.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(19389..19580)
                     /gene="bcsF"
                     /locus_tag="ACHKYO_RS16265"
                     /old_locus_tag="pgaptmp_003253"
     CDS             complement(19389..19580)
                     /gene="bcsF"
                     /locus_tag="ACHKYO_RS16265"
                     /old_locus_tag="pgaptmp_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HGU4430046.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(19577..21148)
                     /gene="bcsE"
                     /locus_tag="ACHKYO_RS16270"
                     /old_locus_tag="pgaptmp_003254"
     CDS             complement(19577..21148)
                     /gene="bcsE"
                     /locus_tag="ACHKYO_RS16270"
                     /old_locus_tag="pgaptmp_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HGU4430047.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            21421..21609
                     /gene="bcsR"
                     /locus_tag="ACHKYO_RS16275"
                     /old_locus_tag="pgaptmp_003255"
     CDS             21421..21609
                     /gene="bcsR"
                     /locus_tag="ACHKYO_RS16275"
                     /old_locus_tag="pgaptmp_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HGU4430048.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         5502 a         5246 c         5675 g         5209 t
ORIGIN      
        1 aagttgaagt acatttcaat ttattgcaaa aactaatctt cagtaaaaat aattttcttt
       61 tttagcacca tttgtataat ctgcgccccg ttgtatgaac aacatcaagc atatttatct
      121 atataaatgg agttataaat gaaaaaggtt ctgtttgcct tatctgcgct tgcgctgacc
      181 tctacttccg tattcgcagc tgatgcgggc gacggtactg ttaaattcac cggtgagatt
      241 gttgacgccg cttgtgttgt gtctccagac actcaaaaac aagaagttgt tctgggtcag
      301 gtgaacaagt ctgtatttac tgctatgggt gataaatcca cagcaacgcc gttcaaaatc
      361 aaactggaaa actgcgatat ctccactttc aaaaatgtgg aaatcagctt taacggcgtt
      421 ggcgatgcag acaacagcaa actggtttct gtaagcactg aaccaggtgc cgcaactggc
      481 gtaggtatcg gtatttatga taacaccaat accctggttg acctgaatac tggtaaatct
      541 gctactgtcc tgaaagaagg tcagaccgtt ctgtatttca ccgctaacta tgtctctacc
      601 aaagatgcag taaccacggg ttacggtaac gctgaagtcg acttcaacct gacttacaac
      661 taatcgatcc tgacggaacg atcatctctc aatggcaaca gagactgtgt tgccattatt
      721 cacaccaatg actcagggac cgaatcatga aacgtattag ctttgctgtg ctggcacttt
      781 taatgaccgc acaacagtgt tttgctggtg gcgtagcctt aagcacgacg cgtgttattt
      841 atgatggcgg caagaaagaa gcgtcgctga ccgttgaaaa ccataataaa aatgaagagt
      901 ttttgatcca gtcatggatt gatgatgtga atggcaataa aaaaacaccg tttattatta
      961 caccgccatt attcaaactt gatcctgata aaaataacgt cttacgtatc gtcaatattg
     1021 aagatacctt acctaaagat cgtgaatccg tttactggat taatgtaaag gccattcctg
     1081 cgaaaagtgc tgaaaatgaa aataaaaacg tcttacagat agccgttcgc acccgtttga
     1141 agttgttcta tcgtccaaaa ggtttgccgg gaagcaacct cgaggccatg aaacagttac
     1201 gtttccagtg tcaaaataac caaattgtgg ttaataacga ctccgcttat aacctgacct
     1261 ttaatcagtt ttccattaat ggacaaaaga ttgaacgggc gggaatggtt ctcgcgaaag
     1321 gaaagttaaa cattaacctt ccagccggta caggcaacgc gcacgaagtg aaatacagca
     1381 ttattaatga tttcggtgtt gctggcgaaa tgctgacgaa gccgattaac tgatcctttt
     1441 acaaggcgct tttactatgc attggatgca tttacctctc tatcactaca gagcgcattt
     1501 ttccttctct ctgctggcat taacgattgc cagctcgtta cctgcgtatg gcggaaaatt
     1561 taaccctaaa tttctcgaaa acgttcaggg tatcgatcaa cacatcgact tgtcggtata
     1621 cgactcacct gtcggccagc aaatccccgg taaataccgg gtttcggtgt ttgtaaacga
     1681 agaaaaaatg gcttcccgga cgctggattt tagtaccgcc agcgaagcga aacgcaaagc
     1741 cagcggtgag tcgcttatgc cttgccttag ccgggtgcaa ctggaagaaa tgggcgtgcg
     1801 cgttgacagc tttccggcgc tgaagatgtc accaccagaa gcctgcgtag cgtttgatga
     1861 aatcattccc caggccacca gccgctttga ttttaatact cagacgctgc atctttcctt
     1921 tccacaggcg gcaatgatga tgaccgctcg cggcacggtt gatccttctc gctgggacga
     1981 aggaatacct gcgctgttgc tggattacag ctttagtggc agcaatggac gcaatgaagg
     2041 tacaggttca tcttcagatt cgaccagtga cagttattac ctgaacctgc tcagcggttt
     2101 gaacattggt ccgtggcgtt tacgtaataa cagtatctgg aaccgtaccg atggcaaaaa
     2161 ccaatgggat aacgttggta catcgttaaa ccgggcaatt attccactta aatcacagat
     2221 aactctgggt gataccgcca cgcctggcga gatttttgat agcgtacaaa tgcgtggggc
     2281 gttactggca tccgacgatg aaatgttgcc ggacagccag cgtggctttg ctccggtagt
     2341 gcgcggcatc gccaaaagca acgctgaagt gagtatcgaa cagaacggct atgtcattta
     2401 tcgcactttt gttcagccgg gtgcatttga gattaacgat ctctacgcaa cctccggcag
     2461 cggcgacctg acggtgatca ttaaagaagc cgacggaagt gaacaacgtt ttatccagcc
     2521 gttttcggcg gtggcgattt tccagcgtga agggtatctg aaatacagtc tggctgccgg
     2581 tgaatatcgt gctggtaatt acgacagcga taaaccacat ttcgggcagt ttaccgccat
     2641 gtacggcttg ccgtggggta tgaccgccta cggtggtgca ttgttgtcgg cagattacaa
     2701 cgctttagct ctgggcctgg ggaaaaactt cggtaccatt ggcgcagtat cggtggatgt
     2761 cactcaggcg aaaagtcagt tacgcaacaa tgaaaaagag gaaggccagt cttatcgctt
     2821 cctctattcg aaaagttttg agggtggcac tgacctgcgt ctgttgggct acaaatactc
     2881 aaccagcggc tactacacct tccaggaagc aactgatgtt cgcagtgacg ccgacagtga
     2941 ttaccgccgt tatcacaagc gcagccagat acagggcaat attacccagc aactgggaga
     3001 ctacggctcg gtttatttca atatgacgca gcaggattac tggaacgttg acggtaagga
     3061 aaactcctta agcgccggtt atcacggtca cattggtcgg gtgaattaca gcgtggccta
     3121 tacctggacg agaagtccgg aatgggaaga agacgatcgc ttgtggtcat tcagtgtttc
     3181 catccctctg gggggcgcgt ggagcagtta tcgcatgact accgaccaga atggcaagac
     3241 gtcgcaacag gcctctgtta gcggcacgtt actggaagat cgtaacctga gctataacgt
     3301 tcagcagggt tacaccagta atggcgtagg ctacagtggc agcgtcaaca tggggtatat
     3361 gggtggctct ggtaacattg acgtaggcta taactattct aaagataacc aacaggttaa
     3421 ctatggcgtg cgcggcggtg tcatcgttca cagtgaaggt ataacgcttt cgcagccttt
     3481 aggcgaaagt ctggcgattg tttctgcacc gggcgcgcgt ggtggacatg tggttaacag
     3541 cagcggcgta gaagttgact ggatgggcaa cgccgtggtg ccttatctca caccatatcg
     3601 tgaaaccatt gtggaattgc gtagcgacac gttggggcaa aacgttgaac tacaggaagc
     3661 gttccagaaa gtcgtgccta ctcgtggtgc agttgtgcgt tcccgctttg atacccgcgt
     3721 cggttaccgt gtgctgatga gcttaaaaca ggcgaatggc aacgcagtac cgtttggtgc
     3781 aaccgcagcg ttaattgatg agagcaaacc tgccagcagc attgtgggtg aagaaggaca
     3841 gctttatatc agcgggatgc cagaagaggg cgaattgcag gtgagctggg ggaacgagca
     3901 ggcgcaacgt tgccgtgtgc cattccgctt gccagagaac aaggacaatg cggctatcgt
     3961 gatggttaat gcggtatgcg agaaataagg atatttagat gaaaattaaa aatttgttgg
     4021 caggtatggt gctgttaggg acgagcactt ttgctggaaa tatatgggct gcggattggg
     4081 gaccatgtca gacatcaaat ggggtggccc atgagtatag ttttgatttc gtacagacta
     4141 tccaggtacc ttcagaaaat aaggctggga aaatattaac tcagcccttt gctttaggta
     4201 caaaatattc cgcttattgt gaatgcccgg atcccattcc agataatgga gttgtgactt
     4261 attttaaagg tgtaacgctt ctgcctgagc ctggaactgt agatgggtac tataaattca
     4321 ataattacat tgatattctc accaaaatat atgtctacac acaattagat atcccggtgc
     4381 cttttaccga tcgaagcaat ggcacggcac agaaagaatg tacgccctat actgctaaca
     4441 attgggggac aggtggtaaa ggaagtatca gtatttatat ttcacatcct tttgttgggc
     4501 aaatgattat tcctaaaaca cgagttgcgt ccctctttgg gacaaaaaag aaaggtgttt
     4561 acaatgatag tcagcccatg gccaatgtca gtatttccgg gagtattaca gttactcaag
     4621 gctgtgaact tgccgctggc accgtactgg atattccatt cggagaatat caggcccatg
     4681 attttaaagg gcgtgccgga caaccaccac aaaatgttca gaaggtacaa aaagaattaa
     4741 gctttgactg caataatatt tcagatggcg tcaaaattta tctcagtatt gatgcaacac
     4801 caaatactgc ctatcccagc gcaattgatt taggcaacgc cgatgttggt gcagtcattg
     4861 aggacggtaa aggtaatatt cttaagccga atgacagtaa tagcttatta gaaatgaacc
     4921 caggctcgct gtatgaagat gtcaaacgca aagcgacaac cacaattacc gcttatcccg
     4981 ttagcactac cggaaaatta cccgctgccg gggattatag cggtattgct accatacatg
     5041 tcgaactgga gtaacagtca tgaagcgaac aatacttgcg ttatgcctgc tgcctctttc
     5101 cttttcgtca ttcgcggcaa ccacggattt agggccgaaa ggaacattga aattcaccct
     5161 taaaatcagc cagggtgctt gcgaactggt gaaagacagc gttgaggttg acatgggcac
     5221 cgcggttctc aaaaagccgg taagaatcgg aacggaaatt aatcccactc ctttttccat
     5281 cggcctgaaa aactgtagcg aagtggtcag agcctacgtg actatggacg gtacacccaa
     5341 tgcagacgac tcaaatcttt ttgcgctgga tgcgggtggc gctacaggcg ttgggctgaa
     5401 aatcaaaacc gctgctggag tacaacagtt cccggaaaat acaacgccga caccggttga
     5461 gtttgcaatt aacggtgatg gcgcacatca actgaactat atcgccagct acgtgcctat
     5521 tcgcgccgat gcaactattg gtcgcgcaga tgcaacggtc gactttactg tacagtacga
     5581 ataatttcac atcttaaggg ccagttaact ggcccttttt actgcttttt gtaacgctaa
     5641 agtctctttt caaacttgca tttttgtaaa tttgtgcttc atgcacactc tttccccaca
     5701 ctttttcgct ttgctgtggt ctacttattc gcgcgtgtag attttactta tctgactacc
     5761 tccgcacttt tcccctgccg ggcctgaaaa gccgctaagc agggtgttat cacctgtttg
     5821 tccagggttt gtttgcatga gatacatcaa atcgattaca cagcagaagc tgagcttttt
     5881 gcttgcaatc tatattggcc tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag
     5941 ctatgcgcac gattttaccg tctggaaagg cgtttctgct gttgttgaac tggccgccac
     6001 cgtactggtg accttctttt tactacgtct tctatcgctg tttggccgcc gcagctggcg
     6061 tattctggcg tcgctggtgg tgctcttttc cgcaggtgcc agctattaca tgaccttcct
     6121 taatgtggtc attggttatg gcatcatcgc ttccgtcatg accaccgata tcgacctgtc
     6181 aaaagaagtt gttggtctga actttattct ctggttaatc gccgttagtg cattgcctct
     6241 tatccttatc tggaataacc gctgtcgcta caccttgctc cgacaactgc gaaccccagg
     6301 gcagcgtatt cgcagcctgg cggtcgtcgt actggcgggt attatggttt gggcaccgat
     6361 tcgtttgctg gatatccagc agaagaaagt ggagagggcg accggcgttg atttgccgag
     6421 ttatggcggt gtcgtagcga actcttatct gccatcaaac tggctttctg cgttggggct
     6481 gtatgcctgg gcgcgggtcg atgaatcttc cgataataat tcattgctta atccggcgaa
     6541 gaaattcact tatcaggcac cgcaaaacgt tgatgacact tatgtcgtgt ttatcatcgg
     6601 tgaaaccacg cgttgggacc atatgggtat tttcggctat gagcgtaata ccacgccgaa
     6661 actggcccag gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa
     6721 actctcactg cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac
     6781 attaaaagaa cagaacattt tcgcggttct gaagcagtta ggattcagtt ctgacctcta
     6841 cgctatgcag agcgaaatgt ggttctacag caacacgatg gcggacaaca ttgcttatcg
     6901 tgagcagatt ggtgcggagc cacgtaatcg tggcaagccg gtagatgata tgttgctggt
     6961 agacgaaatg cagcaatcgc tagggcgcaa cccggatggt aagcatctga tcattctgca
     7021 taccaaaggt tcgcatttta actacaccca gcgttatccg cgtagcttcg cgcagtggaa
     7081 gccggaatgt attggtgttg atagcggctg taccaaagcg cagatgatca actcctatga
     7141 caactcggtg acctatgtgg atcactttat ctccagcgtg attgatcagg ttcgcgataa
     7201 gaaagcgatt gtgttctacg cagctgacca cggtgagtca attaatgaac gcgagcacct
     7261 gcacggcacg ccgcgtgaac tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg
     7321 gatgtcagat aaatatctgg aaaatccggc caatgcgcag gcatttgcgc agctgaaaaa
     7381 agaagccgac atgaaagtgc cacgccgtca cgtagagctg tacgatacca tcatgggttg
     7441 tcttggctat acttcaccgg atggtggaat taacgaaaac aacaactggt gtcacatccc
     7501 gcaggcaaaa gaggcagcgg ctaactaagc ggcctgctga ctttctcgcc gatcaaaagg
     7561 cattttgcta ttaagggatt gacgagggcg tatctgcgca gtaagatgcg ccccgcattc
     7621 ggtgattggc gcagcctggt agcgcacttc gttcgggacg aaggggtcgg aggttcgaat
     7681 cctctatcac cgaccaaatt cgaaaagcct gctcagcgag caggcttttt tgcatttgaa
     7741 gcccatgagg atgagaacct ccgggggctg ggaggttcga ctcgagcgaa gcgagagaac
     7801 gttgcgccag caacggcccg cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg
     7861 accaaattcg aaaagcctgc tcaacgagca ggcttttttg catctgcagc tcatgaggat
     7921 gagaacctcc gggggcaggg aggttcgact cgacgtaggc ctgataagac gcgtcagcgt
     7981 cgcatcaggc aatggcaccg cacttattgt cggatgcggc gtgaacgcct tattcgacct
     8041 acacgccaca catttccctg ccaaatttgc gccccatatc tcactatcca ttaacggatt
     8101 tgtgacagaa accattgtct ttttttatat ttgcttaaat ctttttttgc gttacttatg
     8161 gctggcgctg gcattttccg ctgtgcgttt tagcgttcat ggtattaatc gctcagatat
     8221 ccaccttttc ctcagatttt tttcactaaa tgcataaaac ttaatcacca aatgttgcta
     8281 aatattaagc tgattgttct ggtcatgttg gagtagcaca aatttctctg ctgcaaatag
     8341 ctgtttgaag tttttttaat ctttgtttgt ggattctcac cgttggtgta aatcccggtt
     8401 ggctgtattg acgttttcac attctgttga cagattgtag gtcacgaggg gcattttatg
     8461 gaggatccgc actgttacac tgatgttaat tagtacggca tccccacctc ataacgttga
     8521 cccgaccggg caaaaaacaa aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa
     8581 caacaaacat cacaattgga gcagaataat gcgtatttcc ttgaaaaagt cagggatgct
     8641 gaagcttggt ctcagcctgg tggctatgac cgtcgcagca agtgttcagg ctaaaactct
     8701 ggtttattgc tcagaaggat ctccggaagg gtttaacccg cagctgttta cctccggcac
     8761 cacctatgac gcctcttccg taccgcttta taaccgcctg gttgaattta aaatcggcac
     8821 cactgaagtg atcccgggcc tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta
     8881 taccttccat ctgcgtaaag gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg
     8941 cgaactgaac gccgatgatg tggtgttctc gtttgatcgt cagaaaaacg cgcaaaaccc
     9001 gtaccataaa gtttctggcg gcagctacga atacttcgaa ggcatgggct tgccggagct
     9061 gatcagcgaa gtgaaaaagg tggacgacaa caccgttcag tttgtgctga ctcgcccgga
     9121 agcgccgttc ctcgctgacc tggcaatgga cttcgcctct attctgtcaa aagaatatgc
     9181 tgacgcgatg atgaaagccg gtacaccgga aaaactggac ctcaacccaa tcggcaccgg
     9241 tccgttccag ttacagcagt accaaaaaga ctcccgtatc cgctacaaag cgtttgatgg
     9301 ctactggggc accaaaccgc agatcgatac gctggtcttc tctattactc ctgacgcttc
     9361 cgtgcgttat gcgaaattgc agaagaatga atgccaggtg atgccgtacc cgaacccggc
     9421 agacatcgct cgcatgaagc aggataaatc catcaacctg atggaaatgc cggggctgaa
     9481 cgtcggctac ctctcctata acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca
     9541 ggcactgacc tacgcggtga acaaagacgc gatcatcaaa gcggtttatc agggcgcggg
     9601 tgtatcagcg aaaaacctga tcccgccaac catgtggggc tataacgacg acgttcagga
     9661 ttacacctac gatcctgaaa aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg
     9721 tttctccatc gacctgtggg cgatgccggt acagcgccca tataacccga acgctcgccg
     9781 catggcggag atgattcagg ctgactgggc gaaagtcggc gtgcaggcca aaattgtcac
     9841 ctacgaatgg ggtgagtacc tcaagcgtgc gaaagatggc gagcaccaga cggtaatgat
     9901 gggctggact ggcgataacg gggatccgga taacttcttc gccaccctgt tcagctgcgc
     9961 cgcctctgaa caaggctcca actactcaaa atggtgctac aaaccgtttg aagatctgat
    10021 tcaaccggcg cgtgctaccg acgaccataa caaacgcgtt gaactgtaca agcaagcgca
    10081 ggtggtgatg cacgatcagg ctccggcact gatcatcgct cactccaccg tgtttgaacc
    10141 ggtacgcaaa gaagttaaag gctatgtggt tgatccatta ggcaaacatc acttcgaaaa
    10201 cgtctctatc gaataattaa aagccatacc cggtggcgct gtgcctctgc gtgcagcgcc
    10261 atccggcagc aatacttcat tcccagcccg acctgggcgg gaattgattt gtgagcaata
    10321 cagacacgca gttccaggct gcgggtcact atagagaatc cgggttatgt tgcagtttat
    10381 tctccgacgt ttgggactcg tcatccccac gtttatcggt attacccttc tcacatttgc
    10441 ctttgtccac atgatcccgg gcgatccggt gatgatcatg gcgggcgaac gtgggatctc
    10501 cccagagcgt cacgcgcagc tgctggctga actcggctta gataaaccga tgtggcagca
    10561 gtatctccat tacatttggg gcgttatgca tggcgatcta ggcatttcaa tgaaaagccg
    10621 catcccggtt tgggaagagt tcgtgccgcg cctccaggcc acgctggaac ttggcgtctg
    10681 cgcgatgatt tttgcgactg cggtcggtat tccggtcggc gtgctggctg cggttaaacg
    10741 cggttccatt ttcgatcaca cagcggttgg cctggcgctg accggctact cgatgccgat
    10801 cttctggtgg ggcatgatgc tgatcatgct ggtttcggtg cactggaacc tgacgcccgt
    10861 ctccggtcgc gtgagcgata tggtgttcct cgatgactcc aatccgttaa ccggttttat
    10921 gctaatcgac accgccatct ggggtgaaga cggcaacttt atcgatgccg tcgcccatat
    10981 gatcttgcct gccattgtgc tgggtactat tccgctggcg gtcattgtgc gtatgacacg
    11041 ctcctcgatg ctggaagtgc tgggcgagga ttacatccgc accgcgcgcg ccaaagggct
    11101 aacccgcatg cgggtgatta tcgtccatgc gctgcgtaac gcgatgctgc cggtggtgac
    11161 cgttatcggc ctgcaggtgg gaacattgct ggcgggggcg attctgaccg aaaccatctt
    11221 ctcgtggccc ggtctgggac gctggttgat tgacgcactg caacgccgcg actatccggt
    11281 agtgcagggc ggcgtattgc tggtggcgac gatgattatc ctcgtcaact tgctggtcga
    11341 tctgctgtac ggcgtggtga acccgcgtat tcgtcataag aagtaagggg acatcatgtc
    11401 acaggttact gaaaataaag tgattagcgc accggtgccg atgaccccgt tacaggagtt
    11461 ctggcactat tttaaacgca acaaaggcgc ggtcgtcggg ctggtttacg tcgtcatcgt
    11521 gctgttcatc gcgatctttg ccaactggat tgcaccctat aacccggcgg aacagttccg
    11581 cgatgcactg ctcgccccgc cagcctggca ggaaggcggc agcatggcgc acttgctggg
    11641 caccgatgac gtaggccgtg atgtgctgtc gcgcctgatg tacggtgcgc gcctgtcgct
    11701 gctggttggc tgtctggtag ttgtgttatc gctgattatg ggcgttattc tcggcctgat
    11761 cgccggttac tttggcggcc tggtcgataa catcattatg cgcgtggtcg atatcatgct
    11821 ggcgctgcca agtctgctgc tggcgctggt gctggtggca attttcggcc cgtcgattgg
    11881 taacgccgcg ctggcactga ccttcgttgc cttgccgcac tatgtgcgct taacccgcgc
    11941 cgccgtgctg gtggaagtta accgcgatta cgtcaccgcg tctcgcgtgg cgggtgccgg
    12001 ggcgatgcgt cagatgttta ttaacatctt cccgaactgc cttgcgccgc tgattgttca
    12061 ggcgtcgctc ggtttctcta acgccattct cgatatggct gctcttggtt tcctcggcat
    12121 gggggcacag ccgccaacgc ctgagtgggg caccatgctc tccgacgtgt tgcagttcgc
    12181 gcaaagcgcc tggtgggtcg tgaccttccc gggtctggcg atcctgctga cggtgctggc
    12241 atttaacctg atgggtgacg gtctgcgtga cgcgctcgat cccaaactga agcagtaaga
    12301 ggttcgagat ggcgttatta aatgtagata aattatcggt gcatttcggc gacgaaagcg
    12361 cgccgttccg cgccgtagac cgcatcagct acagcgtaaa acagggcgaa gtggtcggga
    12421 ttgtgggtga gtccggctcc ggtaagtcgg tcagttcact ggcgattatg gggctgattg
    12481 attatccggg ccgcgtaatg gcagaaaaac tggagtttaa cggccaggat ttgcagcgta
    12541 tctcagaaaa agagcgccgc aacctggtgg gtgccgaagt ggcgatgatc ttccaggacc
    12601 cgatgaccag ccttaacccg tgctacaccg tgggtttcca gattatggaa gcgattaagg
    12661 tgcatcaggg cggcaacaaa agtacccgcc gtcagcgagc gattgacctg ctgaatcagg
    12721 tcggtattcc cgatccggca tcgcgtctgg atgtttaccc gcatcagctt tccggcggca
    12781 tgagccagcg cgtgatgatc gccatggcga ttgcctgtcg gccaaaactg ctgattgccg
    12841 atgaaccgac caccgcgctg gacgtgacca ttcaggcgca aatcatcgaa ctactgctgg
    12901 agctacagca gaaagagaac atggcgctgg tgttaattac ccatgacctg gcgctggtgg
    12961 cggaagcggc acataaaatc atcgtgatgt atgcaggcca ggtggtggaa accggtgatg
    13021 cgcacgccat cttccatgcg ccgcgtcacc cgtatactca ggcattgctg cgtgcgctgc
    13081 cagaatttgc tcaggacaaa gaacgtctgg cgtcgttgcc aggtgtcgtt cccggtaagt
    13141 acgaccgccc gaacggctgc ctgcttaacc cgcgctgccc ctatgccact gacagatgtc
    13201 gcgctgaaga accggcgctg aatatgctcg ctgatgggcg tcagtccaaa tgccattacc
    13261 cacttgatga tgccgggagg ccgacactat gagtacgcaa gaggccacct cgcaacaacc
    13321 gctgttgcag gctatcgacc tgaaaaaaca ttatccggtg aagaaaggca tgttcgcgcc
    13381 ggaacgtctg gttaaagcgc tggatggcgt ttcgtttaac cttgaacgtg gcaaaacgct
    13441 ggcagtagtg ggcgaatctg gctgcggtaa atcgaccctc ggtcggttgc tgacgatgat
    13501 tgaaatgccc accggtggcg agctgtatta ccaggggcag gatctgctta agcacgatcc
    13561 gcaggcgcag aagctgcgtc ggcagaaaat ccagatcgtc ttccagaacc cttacggttc
    13621 gctgaatccg cgtaaaaaag ttgggcaaat tctcgaagag ccgctgctta ttaacaccag
    13681 cttaagcaaa gaacagcgtc gggaaaaagc cctgtcgatg atggcgaaag tcggcctgaa
    13741 aaccgagcac tatgaccgct atccgcatat gttctccggc ggacaacgtc agcgtatcgc
    13801 tattgcccgt ggtctgatgc tcgacccgga tgtggtgatt gccgatgagc cggtttctgc
    13861 gctggacgtc tcagtgcgtg cgcaagtgct gaatctgatg atggatttac agcaggaact
    13921 ggggctttct tatgtcttta tctcccacga cctgtcggtg gtggagcaca ttgccgatga
    13981 agtgatggtg atgtacctgg gccgctgcgt ggagaaggga acgaaagacc aaatcttcaa
    14041 taacccgcgc catccgtaca ctcaggcgct actctccgcg acgccgcgcc tgaacccgga
    14101 cgatcgccgc gaacgcatca aactcaccgg tgaactgcca agcccgctga atccaccgcc
    14161 gggttgcgcc ttcaacgccc gctgtcgtcg gcgcttcggc ccctgcaccc agttgcagcc
    14221 gcagctaaaa gactacggcg gtcaactggt agcttgtttt gctgttgatc aggatgaaaa
    14281 tccgcagcgt taactcacaa aaccggggcc atgcgctccg gtttactcaa tcagcttcaa
    14341 cagtggcgac aagcatagca aaatgccgta aagcagaatt agccaggctt taaatccgcg
    14401 aagtttttcc agttgtgcga ctttatagat caggaaaaac ggaatgaggc acgacacaat
    14461 gccatataac gggcttccca actgaaagaa caccagcacc gatacacgaa acgaaaccca
    14521 aatcgtcaat gtaatgacga taaaagcgca gatcgccaga gtcagcatgc gtgagttaat
    14581 tttcttggta tcaataattc ggcttaacag attgagaata atgcctttaa tagcctcgtg
    14641 gaaaccgaga taaatgccaa agaatgcggt cagtacggca aagatattca gcaccgtaga
    14701 ggtgatatga atgatatgtc cagggatcac ctgcgcggcc agcgccagtg ccgagatatt
    14761 ttgttcaaag gcagaaacgg cttcttcgtg gctaattgag aaggtaaacg aaaaggcaaa
    14821 aaacaggatc accgcgatga gcgtgatata actaattcgg tgggtacgca gcgcgagccg
    14881 ggttgccagt actttatccg cttcccgttt acgataggca atattcattg ggttaagtac
    14941 ctgaataaat actgcagaaa agaagcaaaa tggaatggta agcaagacat cgcggaaaaa
    15001 gacggaggct tgcgggaagg cggttatatt ggcgaaattc cagtgcggga tcatcgcaaa
    15061 accgaacacg acaataatcc ctactttgac caccaccatt ggcccggaaa tcttaaacag
    15121 taatcgttca ccaccagacg caatcgccac cagtacggcg aaaatagcga ctttataaag
    15181 tagagattgt gaaagatcgg catcggttaa accgaaggtt ttcaggtacg aggcgctgtc
    15241 gaaaaccacg gagagagagt agataaaaat cccgtggata atcatcaaaa agtagataac
    15301 cccgaggaaa attccccagt tcttccccag gtaatgactg ataatatcgg tgtagtcatt
    15361 acaggaatcg ctttcagaaa gggtttttaa ataaatgtcc tgcactaccc aggtggcagg
    15421 ataagcaatg atcgctgcgg taataaatac ccaaattccc ttcaagccaa tttgtactgg
    15481 catcagcacg gttccggcac caatcgccat gcctatgcat aataaaaccc agccgaagtc
    15541 gtagcgtgta aacggcaatt tctgattgtg tttcgatagt gtgttgtgct gcataaagtc
    15601 ctgtccagaa agaagtcggc ctcgcaaaaa tgaataaacc cagtttctct tgcgcaacag
    15661 gtggagaagt cgaatacgca gtatgtaaaa aatgaggcaa cagattcact ggggaggtcg
    15721 cgaggaggcg caattctaca aaattgcgca tttcactacc agtctggagt cagcccggat
    15781 atgaactgca actattgcaa ataaagaaat taagctggaa ggcatcgcgc aatgaggaag
    15841 attgccgggt gctgacagta aaaagcaatc gtaaaatcag cggcaagccg aaagggttcc
    15901 ggtgaggcgc aatgttgcgg gggctttatc cctggtggca ttggttgctg gagagagaaa
    15961 acccccgcac gttgcaggta tgtacctgac aacataacgg gggctaatct tgaacctaga
    16021 ccactcaaga atagccgcga aacgttgtca ttacaacaca ggcggctata tgacgctcgc
    16081 agagctgggc atggccttct ggcatgattt agcggctccg gtcattgctg gcattcttgc
    16141 cagtatgatc gtgaactggc tgaacaagcg gaagtagcgt gtcatgtggg cgtcaggctg
    16201 ccgtaatggc aatttgcgcc cggaccaggc cgcaggagtg aaactctgcg gcctttttcg
    16261 taagaaaaat gcaacggttg caagcggata atattctgcg gaatgcgcag cacagagtga
    16321 agatgagacg gattccggaa cagaaaaaca gtcgtagaat cagcgtcaag ccggaaggat
    16381 tccggtgagg cgcaatgttg cgggggctta atccctggtg gcattggttg ctggagagag
    16441 aaaacccccg cacgttgcag gtatgcacct gacaacacca cgggggccat tcttgcatcc
    16501 aaacaataca agaatagccg cgaaacgttg tcattacaat acaggcggct atatgacgct
    16561 cgcagaactg ggcatggcct tctggcatga tttagcggct ccggtcattg ctggtattct
    16621 tgccagtatg atcgtgaact ggctgaacaa gcggaagtaa cgtgccatgc gggcgtcagg
    16681 ctgccgtaat ggcaatttgc gcccggatca ggccgcaggg gtgaaactct gcggcctttt
    16741 tcgtaagaaa aatgcaacgg ttgcaagcgg ataatattct gcggaatgcg cagcacagag
    16801 tgaagacgtg acggattccg gaacagaaaa acagtcgtag aatcagcggc aagccggaag
    16861 ggttccggtg aggcgcaatg ttgcgggggc tttatccctg gtggcattgg ttgctggaga
    16921 gagaaaaccc ccgcacgttg caggtatgca cctgacaaca tcacgggggc cattcttgaa
    16981 cctgaacaac tcaagaatag ccgcgaaacg ttgtcattac aacacaggcg gctatatgac
    17041 gctcgcagaa ctgggcatgg ccttctggca tgatttagcg gctccggtca ttgctggcat
    17101 tcttgccagt atgatcgtga actggctgaa caagcggaag taacgtgtca tgcgggcgtc
    17161 aggctgccgt aatggcaatt tgcgcccgga ccaggccgca ggggtgaaac tctgcggcct
    17221 ttttcgtaag aaaaatgcaa cggttgcagg cggataatat tctgcggaat gcgcagcaca
    17281 gagtgagtaa gtgatggatt tcggaacaga aaaacagtcg tagaatcagc ggcaagccgg
    17341 aaaggttccg gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg
    17401 agagagaaaa cccccgcacg ttgcaggtat gcacctgaca acataacggg ggctaatctt
    17461 gaacccaaac aactcaagaa tagccgcgaa acgttgtcat tacaacacag gcggctatat
    17521 gacgctcgca gagctgggca tggccttctg gcatgattta gcggctccgg tcattgctgg
    17581 cattcttgcc agtatgatcg tgaactggct gaataagcgg aagtaacgtg tcatgcgggc
    17641 gtcaggctgc cgtaatggca atttgcgccc ggatcaggcc gcagaggtta aactctgcgg
    17701 cctttttcgt gcttactgcg ggtaaggcac ccagtcgccg ccgttcaggc gaacgtacgg
    17761 tttatcctgg tattgaataa ctactgcatt tgagttctcg gagaccggtg ctgtttgtgg
    17821 caacccactg gtgagttttt tccagtcaac attgtcttcg gtgaaaatct tgccatcaag
    17881 aacgcgaacc accagatcgg agatagccag gaagctgctc ggttggtcaa tgacaatcgg
    17941 tgccccctga tgtggtgcct tcatgccgaa gaatttcacc ccaacgggga cgtctgtgat
    18001 agacgggcta gggatatcac gtaggccaga tacctgcatt ctgtcgccct tcagcgcgcc
    18061 gccgtgttcc ggcaccacga ccaccatcac tttacgaccc gatttctcca gttcagtaaa
    18121 gaaggcgtcc agttcatcaa agaatttctg cgcccgcgct ttgtaatccg ctgttttgct
    18181 gacccccgga taatggttgc cgtcatgcag tggaagcgtg ttgtagaacg tggcactacg
    18241 gctgttttta tctttttcgg taacgtccag ccagcggtta agcacggcgg tatcgtcata
    18301 aaccggcgaa ccatcaaagc ccagcaaaat aaccggcaga tttgtttgat ccatcaattc
    18361 agtctgcatg ccgccatttt cgcgaacttc tttcaaaaaa ccgccgaact ggccgttatg
    18421 ccccatcatc aggtgctggg taaagcccag tttcgaaagg ttatcaaaca gatagcagtc
    18481 gttatttgcc ggttgataca gattagtgtg cgaagtctgc ccgcagctgg cgcgcagtaa
    18541 acggatcgcc gccgggccac tgtaggaggt ggcggagtta aagttcttga actcaatatc
    18601 gaaatgcgac cacagtggat gcgacatcaa cccggcggct tctatatccg accaggaaag
    18661 cgaacagatg ttaatcacca gtagttcaaa tggctgagca tcagcgggca gcgaagacgg
    18721 gaaggtcgat ttacgtttcg cctccgcgtt atagaaatta ttcagccagg cgttaaggtt
    18781 cgccgttgtt ggcggtgcag tttgtgcggg catatcaccc actaccggtg cgccacccgt
    18841 cgccgcaacg gttgccgctg cgttaccacc cgtcgttgtt acagtggtcg tcggttgtcc
    18901 ggctggccac aaggagaaac ttggtcccgc cagggtaagt acgttcagcc atagcagtat
    18961 ggcaaccaca aaaacggtaa tgcgaatcca ttgtgacagg aataaccagg ccactaataa
    19021 aacaaaaatg gccccaatca tctgccagtt aataaagcgt gtgacaaggt cgattaaata
    19081 atcggtactg aaccccgcca cctgcgaacc ctggctcatt atgctttccg ggccaggcaa
    19141 ccaggtgtca tgccagaaca aagcaaagcc gatcggcagg gcaatccagt ggcgcaagcg
    19201 atgcaggctg tagcgcggaa tgggcatcag cagaaacgcg gcaaacacca aattgaggag
    19261 cggatggaag ttaagatatc ccgcccacaa caggccgaac ttaaccagaa aatagaagtt
    19321 ccagccggaa aggccgcgcc agtattgcca gagggaagaa ggcatggcgg tattttgcgt
    19381 aaattgagtc attttttggt tgccctggct ttttccgtgc ggcgtaacgt cccggccggt
    19441 ttaacataac gaggtttagc aaagaacaaa cgtaaggtgt cgcgaatgcg tcgcaaagag
    19501 tgccgcgcca gatagcccag cgggaaaaat atcagtgcgc aaacgacaat aatttcaatg
    19561 atatcgctga tggtcatcat gatgagcgct ccacagcatc atctaacagt cgcatgggtt
    19621 ctggtattcg tcgccagtgg cgaccatcgt gctcggcatt gatgaccggt ttagaacttt
    19681 gcgttaaagg cagcggcatg ccccattgtt ctggggcaag caagcgcatc tgcaccagct
    19741 cggcactgat ttgatcatct tcaaaccaga ccatacggtt tgagaaaatg tcgccagtag
    19801 gcaatgggaa aatatgattc aacgcggtat ccagatcgtt aatccgacag aatgagagaa
    19861 acagcaccag ccgattaccg ccaatggtca tgatatcgcc ggtacggtta gggcgacaca
    19921 gcgtcagggc ttgttcaacg cggatacccg gtaccggacg tagggcaacc agaacgcctt
    19981 taccgtgggc aggtaataga gggttattca tcatgttgtt gacggcatta cagaacacat
    20041 cccacttctg gaaaccacgc agtttgagcg gctgggtcat tgacagcaag gtagtgatat
    20101 cttccggcac atagcgacta aacttctgcc cttgcacgct ttcgatcatc gtcagacaac
    20161 gggagagtgg cgcattccac ggaataacca tatttgcacc gcaggccaat aacaaacgtt
    20221 catcggtggc gcgcaggcta gcggtatttt cccgcacgag gattttcatc gcactaccgc
    20281 gctggcgacg cagggtatga atgctgcggg ccagtggctc gatttgcgca ttttgctgta
    20341 aagaaaagac caccgtcgcc gcctgagcgg tacgggcttc attgaacagg acttcgttat
    20401 tgttgaacag ttgccagtgt tccgatagcg gcggcgcacc ttccagtaca gcaacattac
    20461 tcagaatgcg tttttcgtcg ctgcgtggtt ggatctccgc ctcttcgctt tgaactaatg
    20521 tccagatacc attttgttgc tgaacgctaa gctgctgacg ggcgctgacc cctttttcgt
    20581 tgcaccagaa ggcaatatcc agcaaatgtt ggtcaccctg aaaacgcaaa ctggcaagac
    20641 caaaaagtga acggtattcc tcaagcaaca atgaaaattg tttatcgtta ttatttccgg
    20701 gattaattac caaaagcgaa caatggttta acctgctcca tttattcatt ttatccaacc
    20761 atgagcgaag ccgctcggca ggaatgtttt gccatgcgtt atttgcgcaa acaagaataa
    20821 aaaggtaatt atggggatca atagaacact gcaaatcacg ggtcaaatag tatagacctt
    20881 tttcatgatt tagcattgaa aataatttta ttttttccgg cccttgagaa tcatctaatt
    20941 gaaagatttt cgccggatcg ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct
    21001 gggatgcaat tgtttgattc gccagactga tagcatcttc atggcgatcg acgttaaacc
    21061 accagacgcc gcctgctggc atatgtcgca gctcatccca taatgatgag ataccgatag
    21121 agaatacagg gtccacaatg tccctcatgt caccattttg tctgattatc agtttactag
    21181 cgaaagctaa gaaataaacc taccattgaa attaaaactt atcagattta gcatgtgaat
    21241 caatttagaa ggtcacaaga cgtgtgattt tagtcttttc gtttttaact ttttctgttg
    21301 tgtaacggaa agtcaaaaag tgagcaaatt cccgtctcgc cgtaaacaat aaaccggcga
    21361 aatgctcacg gttagaattg tctcatgaac ggtacggtta tttcataggg atcaagcaaa
    21421 atgaataaca atgaaccaga tactctgcct gatcccgcga taggctatat cttccagaat
    21481 gatattgtgg cgttaaagca ggcattttca ctgcctgata ttgattatgc cgatatttcc
    21541 caacgcgaac agttggccgc ggcattaaaa cgctggccgt tgctggcaga gtttgcgcaa
    21601 caaaagtagg ggattggtga atggccgtac tg
//
LOCUS       DAZGAN020000078        21516 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGAN020000078 DAZGAN020000000
VERSION     DAZGAN020000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.078"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..547)
                     /locus_tag="ACHKYO_RS16280"
                     /old_locus_tag="pgaptmp_003256"
     CDS             complement(<1..547)
                     /locus_tag="ACHKYO_RS16280"
                     /old_locus_tag="pgaptmp_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU4430049.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYN"
     gene            complement(745..1218)
                     /gene="sbmC"
                     /locus_tag="ACHKYO_RS16285"
                     /old_locus_tag="pgaptmp_003257"
     CDS             complement(745..1218)
                     /gene="sbmC"
                     /locus_tag="ACHKYO_RS16285"
                     /old_locus_tag="pgaptmp_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HGU4430050.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEITGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(1337..2503)
                     /gene="dacD"
                     /locus_tag="ACHKYO_RS16290"
                     /old_locus_tag="pgaptmp_003258"
     CDS             complement(1337..2503)
                     /gene="dacD"
                     /locus_tag="ACHKYO_RS16290"
                     /old_locus_tag="pgaptmp_003258"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HGU4430051.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            2712..4139
                     /gene="sbcB"
                     /locus_tag="ACHKYO_RS16295"
                     /old_locus_tag="pgaptmp_003259"
     CDS             2712..4139
                     /gene="sbcB"
                     /locus_tag="ACHKYO_RS16295"
                     /old_locus_tag="pgaptmp_003259"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HGU4430052.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(4227..4409)
                     /locus_tag="ACHKYO_RS16300"
                     /old_locus_tag="pgaptmp_003260"
     CDS             complement(4227..4409)
                     /locus_tag="ACHKYO_RS16300"
                     /old_locus_tag="pgaptmp_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HGU4430053.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDY"
     gene            complement(4423..5481)
                     /gene="tsuA"
                     /locus_tag="ACHKYO_RS16305"
                     /old_locus_tag="pgaptmp_003261"
     CDS             complement(4423..5481)
                     /gene="tsuA"
                     /locus_tag="ACHKYO_RS16305"
                     /old_locus_tag="pgaptmp_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HGU4430054.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIVKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(5660..7018)
                     /gene="plaP"
                     /locus_tag="ACHKYO_RS16310"
                     /old_locus_tag="pgaptmp_003262"
     CDS             complement(5660..7018)
                     /gene="plaP"
                     /locus_tag="ACHKYO_RS16310"
                     /old_locus_tag="pgaptmp_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HGU4430055.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(7008..7070)
                     /gene="yoeI"
                     /locus_tag="ACHKYO_RS16315"
                     /old_locus_tag="pgaptmp_003263"
     CDS             complement(7008..7070)
                     /gene="yoeI"
                     /locus_tag="ACHKYO_RS16315"
                     /old_locus_tag="pgaptmp_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HGU4430056.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(7285..8214)
                     /gene="yeeY"
                     /locus_tag="ACHKYO_RS16320"
                     /old_locus_tag="pgaptmp_003264"
     CDS             complement(7285..8214)
                     /gene="yeeY"
                     /locus_tag="ACHKYO_RS16320"
                     /old_locus_tag="pgaptmp_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4430057.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(8260..9084)
                     /gene="yeeZ"
                     /locus_tag="ACHKYO_RS16325"
                     /old_locus_tag="pgaptmp_003265"
     CDS             complement(8260..9084)
                     /gene="yeeZ"
                     /locus_tag="ACHKYO_RS16325"
                     /old_locus_tag="pgaptmp_003265"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4430058.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(9167..9421)
                     /gene="yoeB"
                     /locus_tag="ACHKYO_RS16330"
                     /old_locus_tag="pgaptmp_003266"
     CDS             complement(9167..9421)
                     /gene="yoeB"
                     /locus_tag="ACHKYO_RS16330"
                     /old_locus_tag="pgaptmp_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HGU4430059.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(9418..9669)
                     /gene="yefM"
                     /locus_tag="ACHKYO_RS16335"
                     /old_locus_tag="pgaptmp_003267"
     CDS             complement(9418..9669)
                     /gene="yefM"
                     /locus_tag="ACHKYO_RS16335"
                     /old_locus_tag="pgaptmp_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HGU4430060.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            9952..10002
                     /gene="hisL"
                     /locus_tag="ACHKYO_RS16340"
                     /old_locus_tag="pgaptmp_003268"
     CDS             9952..10002
                     /gene="hisL"
                     /locus_tag="ACHKYO_RS16340"
                     /old_locus_tag="pgaptmp_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HGU4430061.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    9978..10101
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            10148..11047
                     /gene="hisG"
                     /locus_tag="ACHKYO_RS16345"
                     /old_locus_tag="pgaptmp_003269"
     CDS             10148..11047
                     /gene="hisG"
                     /locus_tag="ACHKYO_RS16345"
                     /old_locus_tag="pgaptmp_003269"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HGU4430062.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            11053..12357
                     /gene="hisD"
                     /locus_tag="ACHKYO_RS16350"
                     /old_locus_tag="pgaptmp_003270"
     CDS             11053..12357
                     /gene="hisD"
                     /locus_tag="ACHKYO_RS16350"
                     /old_locus_tag="pgaptmp_003270"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HGU4430063.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILCAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALSASRQIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            12354..13424
                     /gene="hisC"
                     /locus_tag="ACHKYO_RS16355"
                     /old_locus_tag="pgaptmp_003271"
     CDS             12354..13424
                     /gene="hisC"
                     /locus_tag="ACHKYO_RS16355"
                     /old_locus_tag="pgaptmp_003271"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGU4430064.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            13424..14491
                     /gene="hisB"
                     /locus_tag="ACHKYO_RS16360"
                     /old_locus_tag="pgaptmp_003272"
     CDS             13424..14491
                     /gene="hisB"
                     /locus_tag="ACHKYO_RS16360"
                     /old_locus_tag="pgaptmp_003272"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HGU4430065.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELMKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGITGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            14491..15081
                     /gene="hisH"
                     /locus_tag="ACHKYO_RS16365"
                     /old_locus_tag="pgaptmp_003273"
     CDS             14491..15081
                     /gene="hisH"
                     /locus_tag="ACHKYO_RS16365"
                     /old_locus_tag="pgaptmp_003273"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HGU4430066.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVHKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            15081..15818
                     /gene="hisA"
                     /locus_tag="ACHKYO_RS16370"
                     /old_locus_tag="pgaptmp_003274"
     CDS             15081..15818
                     /gene="hisA"
                     /locus_tag="ACHKYO_RS16370"
                     /old_locus_tag="pgaptmp_003274"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HGU4430067.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            15800..16576
                     /gene="hisF"
                     /locus_tag="ACHKYO_RS16375"
                     /old_locus_tag="pgaptmp_003275"
     CDS             15800..16576
                     /gene="hisF"
                     /locus_tag="ACHKYO_RS16375"
                     /old_locus_tag="pgaptmp_003275"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HGU4430068.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITHLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            16570..17181
                     /gene="hisIE"
                     /locus_tag="ACHKYO_RS16380"
                     /old_locus_tag="pgaptmp_003276"
     CDS             16570..17181
                     /gene="hisIE"
                     /locus_tag="ACHKYO_RS16380"
                     /old_locus_tag="pgaptmp_003276"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HGU4430069.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(17279..18256)
                     /gene="wzzB"
                     /locus_tag="ACHKYO_RS16385"
                     /old_locus_tag="pgaptmp_003277"
     CDS             complement(17279..18256)
                     /gene="wzzB"
                     /locus_tag="ACHKYO_RS16385"
                     /old_locus_tag="pgaptmp_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HGU4430070.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGSAAPKVSEIQSMLIGRFSTT
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAQYIQQVDDQVN
                     EELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(18403..19569)
                     /gene="ugd"
                     /locus_tag="ACHKYO_RS16390"
                     /old_locus_tag="pgaptmp_003278"
     CDS             complement(18403..19569)
                     /gene="ugd"
                     /locus_tag="ACHKYO_RS16390"
                     /old_locus_tag="pgaptmp_003278"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HGU4430071.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(19818..21224)
                     /gene="gndA"
                     /locus_tag="ACHKYO_RS16395"
                     /old_locus_tag="pgaptmp_003279"
     CDS             complement(19818..21224)
                     /gene="gndA"
                     /locus_tag="ACHKYO_RS16395"
                     /old_locus_tag="pgaptmp_003279"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGU4430072.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDSEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQLAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(21304..>21516)
                     /locus_tag="ACHKYO_RS16400"
                     /old_locus_tag="pgaptmp_003280"
     CDS             complement(21304..>21516)
                     /locus_tag="ACHKYO_RS16400"
                     /old_locus_tag="pgaptmp_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430073.1"
                     /translation="IALLQIMQYMIPIMGGYACKFLKASFVFICLFILSYYQRPIFFN
                     LAFSMIFYYSIFTYLKTVGISATNKK"
BASE COUNT         5565 a         5564 c         5271 g         5116 t
ORIGIN      
        1 gattatactc ggtcagactt ttcgccagtt gaatgcgcca gtggatgaac gcccgttgtg
       61 gccagatacc ggtaaacaac attgccagta aagagccgag gatcacatcg ccgcttcgcc
      121 ataacgccgt atcaatttca cctgtcgggg aaccaacaac aattgccagc gtcaccccaa
      181 tcaataaacc ttgatacggt ttcttgccca gcgccagcca accgcaaaga aacatcgccg
      241 ccgcgcacca gactaacatc agcggtaacg aaattaactc cagttgcaga gcgataagac
      301 ctaaaatcga acccaacacc gtaccgccaa tacgttcgaa ggcgcgaggg acaacgttac
      361 cccagaacga gattggcccc ataatcacca ccatggtgac cagcggccag gtgctttccg
      421 ggatagtaaa caggcggata atgagaaaag tgagcaggaa tgccagcgcg acccgagtac
      481 catgcacaat gcggtaatgg cggtataccc ggatttcaaa cgggcttaat gacttatcgg
      541 cacgcacgca gaaactccgt aatgaagatt aaaagcgctc ataactcatc tgtcggccag
      601 aaggcgcacc tctgatttat ccgacggaca taccgggaac agtgtaattt cccggcagga
      661 gcgaaaaatt tgaaaggcgc atcttactct tttcgctttc aaaaaaagag tggtcttcgc
      721 gtcaacacac cgccctgaga tgaattagtg atgttttggc tgcaccgcaa catacatttc
      781 gatatcccag tacccatctt ccgcgccatt gttcaagtaa acctcgaagc agggctttgg
      841 taacatttca taagcactgt cctgcaagag actattaaag aactgatacc agggtttagc
      901 aaaatcatca ccgactacac gagctaccgc caccgcatac tgaccacctg taatttctgt
      961 cagaatgacg ccctcactgt tttcgggaag cgtaaagtaa cccggcaccg tcacgacggt
     1021 gtcgcagcgt aatttttcgg cgggtgtttc atctggattg tcgtaataga cagcaaccca
     1081 ctccttcggc acaatatttt tgttatctac ccacatcatc aactgctcaa agcctttctt
     1141 taccgtctgt tcccacgggc caacgagatg gaaacctgca acggtacgtt tctcttcctg
     1201 cttaatctcg tagttcatga cgcctccatt aacaccgttt ttatatacag tatagttgca
     1261 atattaaaac cacaaggaat gagtgttgat tatgcgagca gactcgcact cctgccagtc
     1321 tgctgcaaaa gaaaggtcag gccttatggt ggaaataatc actcaggcgg gaaaacatgc
     1381 cgccttcccc gacagattcc agggtaacca gcggccagtg cgccacctgt ttatcacggt
     1441 cgtaaagttc aatttcccct acccgctgat gggcgctaat tggcgcggtg agctctttac
     1501 catcaagggt atatttggct ttgatatgtg gaatttcggc tttcggtagc accatccaga
     1561 actcttgttc cgttcccagg tcgatatttt ctttatcgcc ataccagatg cgttccgttc
     1621 cgaccttttt cccacggtgc aaaatttgca ccgtagtaaa gttttgttgc ccccaacgca
     1681 gtaattttct tgcctcttcc tcacgacctt ttgcactgtc agcccccatt accactgcaa
     1741 tgagacgacg ctgcccatct acagccgaag caatgagatt aaacccggca ccagaagtat
     1801 gacccgtttt caggccgtca acattcatgg ttttatccca caacaacccg ttacggtttt
     1861 gctgggtgat accgttccag gtgagacttt tctcactgta catatgataa aactcgggct
     1921 cgccgtggat gatagcgcga gaaagcacag ctaaatcata agccgagcta tgctggccag
     1981 gtgcatccag accatgcact gtttcaaaat gcgtatcctt gagatgcagc ttctcggcat
     2041 agttgttcat catttcaaca aactgccgtt gcccaccggc aatatagtca gccagagcaa
     2101 cacaagcgtc atttccggaa tccacaatta aaccacggct taaatcacgt accgatacgc
     2161 gatcgccctc tttcaaaaac atcagtgaag aaccgacaaa caccggatta tctttcgccc
     2221 acgcatcgcg ccccacggtg acaatatcgt ctggcgtaat gcgatgacta tcgatagcgc
     2281 gatccacgac ataacccgtc atcagctttg tcaggctggc gggattgcgc tgttgatgct
     2341 cattacccgc ggtgaggatc tgaccggtgg tgtaatccat caatacccag gacccggcat
     2401 gaatctctgg aggctgaggt gaaaaaggaa tgttttccgc cgcaaaacca gacgataagt
     2461 taaaaacgaa caaagaagca gcaataataa gacggcgttt caacagcaaa ccctcaggag
     2521 tttcaaatag ctgttctttt tacggaaata cttatgaact ggctggaata aagtgcaaga
     2581 aaatgtgact accctctcat ttttatctga catgatctgt tgccactcgc tgccaaattg
     2641 tggcgctaaa gctgattagc acggtgatat ttgatactct ggcagacagc agaaataacg
     2701 gatttaacct aatgatgaat gacggtaagc aacaatctac ctttttgttt cacgattacg
     2761 aaacctttgg cacgcacccc gcgttagatc gccctgcaca gttcgcagcc attcgcaccg
     2821 atagcgaatt caatgtcatc ggcgaacccg aagtctttta ctgcaagccc gcggatgact
     2881 atttacccca gcctggagca gtattaatta ccggtattac cccgcaggaa gcacgggcga
     2941 aaggagaaaa cgaagccgcg tttgccgccc gtattcactc gctttttacc gtaccgaaga
     3001 cctgtattct gggctacaac aatgtgcgtt tcgacgacga agtcacacgc aacgtttttt
     3061 atcgtaattt ctacgatcct tacgcctgga gctggcagca tgataactcg cgctgggatt
     3121 tgttggatgt tatgcgtgcc tgttatgccc tgcgcccgga aggaataaac tggcctgaaa
     3181 atgatgacgg tctaccgagc tttcgccttg agcatttaac caaagcgaat ggtattgaac
     3241 atagcaacgc ccacgatgcg atggctgatg tgtacgccac tattgcgatg gcaaagctgg
     3301 taaaaacgcg tcagccacgc ctgtttgatt atctctttac ccatcgtaat aaacacaaac
     3361 tgatggcgtt gattgatgtt ccgcagatga aacccctggt gcacgtttcc ggaatgtttg
     3421 gagcatggcg cggcaatacc agctgggtgg caccgctggc gtggcatcct gaaaatcgca
     3481 atgccgtaat tatggtggat ttggcaggag acatttcgcc attactggaa ctggatagcg
     3541 acacattgcg cgagcgttta tataccgcaa aaaccgatct tggcgataac gccgccgttc
     3601 cggttaagct ggtgcatatc aataaatgtc cggtgctggc ccaggcgaat acgctacgcc
     3661 cggaagatgc cgaccgactg ggaattaatc gtcagcattg cctcgataac ctgaaaattc
     3721 tgcgtgaaaa tccgcaagtg cgcgaaaaag tggtggcgat attcgcggaa gccgaaccgt
     3781 ttacgccttc agataacgtg gatgcacagc tttataacgg ctttttcagt gacgccgatc
     3841 gtgcagcaat gaaaattgtg ctggaaaccg agccgcgtaa tttaccggca ctggatatca
     3901 cttttgtcga taaacggatt gaaaagctgt tgttcaatta tcgggcgcgc aacttcccgg
     3961 ggacgctgga ttatgccgag cagcaacgct ggctggagca ccgccgccag gtcttcacgc
     4021 cagagttttt acagggttat gcagatgaat tgcagatgct ggcacaacaa tatgccgatg
     4081 acaaagagaa agtggcgctg ttaaaagcgc tttggcagta cgcggaagag atcgtctaat
     4141 ttgaagcctc cgccgctggt acggcggagg acaatgttga gtcaggcttt ttgaacggtg
     4201 atgctccacg ctgcatcgcc aatctgctaa taatcggtga tggcatgtcc ttcttctgcc
     4261 gcccactgtg ggattgcttc agttgcctgg gtgcaatcaa actcaattac caactcatcg
     4321 ccgctggcca tttctgccag tgctgctttt gcttctatga gtggaaacgg acagacctgc
     4381 gtcaccacat ccagcttttt aatcgccatc tttacacctt acttaatttg ccgcagcagt
     4441 tgccagtcgc gccttacgct gcggtcgaac atacacaagc caggacgcag tccagactcc
     4501 gagaatcata aatacaaggc caatccagcc ctgccaggtc atcattgcag tcataaccag
     4561 tccattaccg atagagcaac cacctgcaat actggcaccg aagcccatca gtacaccacc
     4621 taacccgcta cgtaatgttg tttgtgcatc agctgcgcga acgcggaact cacggctcgc
     4681 tttggctgca ataaatgacc ccacgaagat ccctaacacc aggaaaacgc cccagttaat
     4741 gtatttcacg tcacccgcga ccagaaattg cagaatattg gccgttgggg aagtgattcc
     4801 taacccaaac atgcgtccgg ttgcttcact caggggccag gctaaaagcg caatcaaacc
     4861 gatgagtaca gcagtgacaa agggatgcca gcgtttttca aacagaatat gagcgatccc
     4921 ggttcggcgc ggcggtaagg tcgcgacttt gagttttggc ttcttcaact ctttcatcac
     4981 cacccagagc gttatcacca gcaaaacggc aaccaacggc cacacagaca aattaaaagt
     5041 ctcagcaata gagttatgtt cagtactgta gtgctgcaag gtttgattta aaccactggc
     5101 atgtggagaa cgcatcaccg cactcatcac catataagtg aaaagcgcga tccaactgcc
     5161 gatcaatccc tcacccgcgc gataccaggt cccggtggca catccacccg ccagaacaat
     5221 tcccagcccg aagatatacc cacctataac agtaccaagc cacgggaacg ccccggcttc
     5281 gtaagtcagt aggcccgcct gaattaacgc aaaaaccccc acgctttgta ccgaaattgc
     5341 aatcagcaag gcgtaaaaca tgcgattatt tttcacgata tacatatcgc gaaaaccacc
     5401 tgtcaggcaa aaacgcccac gctgcatgac aaatcccagc agagcaccac aaattagccc
     5461 gcttaatatc attgaaaaca taaattaatt aaccagatga atgttaatag ggaaattatt
     5521 catgactggt ggaatgcaga ccaataacca aattctctaa attagaacaa atggttatta
     5581 atgaggatgt cgataaaaaa gaataaaaaa accggagcca aatattcatt cgactccggt
     5641 ttattattac aaaagatgat tacgctacgt cttcgtactg cggaaccggg ttacggaaac
     5701 tcttggtgac gcaagccagg taaatcagac cgatagccgc ccagatcaga cccagaacca
     5761 ttgagctttc ttccaggtta acccacagcg caccaacggt cagcgcacca cacatcggca
     5821 ggaacagata ctggaagtga tctttcagcg tcttattacg cttctcacgg atccagaact
     5881 gcgagatgac cgacaggtta acgaaggtga acgccaccaa cgcaccaaag ttaatcagcg
     5941 ctgtagccat taccaggtcg aagttgattg ccagcagggc aatcgcgcca accaggatga
     6001 tgttcatcgc cggagtacgc catttcgggt gtacataacc gaagaagctt ttcgggaata
     6061 cgccgtcacg acccattacg tacatcagac gcgctacgcc tgcatgcgcc gccataccgg
     6121 acgccagtac ggtaatggtg gagaagatca gcgcgccaac ctggaacgct ttacccgcaa
     6181 cgtacagcat gatttcaggc tgtgacgcat ccggatcttt aaagcgagag atatccggga
     6241 agtacagctg caggaagtaa gttgcaaaga tgaagatcat gccgccaatc agcgcggtca
     6301 ggaaaatcgc acgcgggatc acgcgctctg catctttggt ttcttccgac aggttgctga
     6361 tgccgtcaaa gccggtaaag gagaagcaca ggattgtcgc cccggtaatc atcgggataa
     6421 catgcgcatc gccagaccag aacggacgag tgctcgccag cgtaccagcg ccttcacctt
     6481 caaatacgcc ataaacaacc atgcccagaa tcaccgcgat cagcactacc tgcaacacga
     6541 cgattacagt gttgaagttc gctacggatt tcagactacg caggttaaag gcggtcatga
     6601 aggccaccag cgccaccaca aacatccacg atgggatgga aggcactaga gcttcaaaat
     6661 agattttcgc cagcagaatg ttgatcatcg gcgcgaacag atagtcgagc agagaagacc
     6721 aacccaccat aaagccgaca gtcgggctaa tggatttctg ggcgtaagtg tatgcagagc
     6781 cagcagaagg atagcggcga accagcttcc cgtagctcag agccgtaaac aggatcgcaa
     6841 tcaatgcgaa cgcataggct gtcggcacat gaccatccgt gaggcctgaa acgataccaa
     6901 aagtatcaaa cagcgtcatc ggctgcatat aggcaagacc catcattaca accggaacta
     6961 acgtaagcgt tttacgcaat tccacgcgag aggtgtttgg agtaacgtta tgcgacatgg
     7021 tcattctcct ttacggtgat aaccgtcgcg taagcaaaaa attgccccat ttttttggat
     7081 tcctcagcga caacaactgt cgatttttag taaatatcta tccggtacga agcccggcct
     7141 cttggtatga atgattggtt tgaagcaaaa aataaccgac gctgatgaaa cgtcggtttt
     7201 tagtcatttt ttgacagcgg cgcattgtgc ctaaatgggg gggaaatgac aagagaatga
     7261 gaggcttgtc ataataattt ttctttaaat ggctgatttt ccgtcatcag attttgcata
     7321 aacaccggcg atcgcactat ttgctaaaat ttcatcccgc caccagccgc tggcaagccc
     7381 tgcggtctgt tcattccagc caatccagac gggttcaaag agagtttggg cgaccacttt
     7441 cttctcgatt aacgcgccac tatcgagaaa ccgttgcgcc agataacggg gtaaatagcc
     7501 gcagccgagg ccgctaattt gcagctccag cttggtttta aaatcgaaaa cggtaatggc
     7561 ttcttgttcg tcgagaagtt gtgaggccgt agaagcgcct gcctgcgcag tatcccccac
     7621 cacaatcgcc cgatatcgct taataatacg acggtttaac ggctcttctt ccagagctaa
     7681 cggatgatga ggcgcgaccg caaagacttg ttccagatcg cccaaccgtg aaaaaccaaa
     7741 ctcactggaa gatggtggct catgcatcgc gccaacaata atatccgctc gcccctgagt
     7801 cagggcatcc caggaaccac cgagtacgcc attgataaac ttcaggcgcg tcacgctatg
     7861 atgttgataa aaagcttcaa tcagtggcgc aagcagagaa aaaggaaacg tgtcgtccac
     7921 gccaatcacc agctcgtttt cccagccttc atgcagtttg atcgcctgct tttccagttc
     7981 tcgcacggta tgcagaactt cccgcccttt ctctaataac atttttccgg tacgggtgaa
     8041 tttagcgcgg tgaccgctac gatcaagcaa ttgaatatta aggtcgcttt ccagcttgtg
     8101 aacggtgtaa ctcagagcgg acggcgtctt atagagtttg gccgacgccg ccgcaaaact
     8161 gccttctttt tctaaggcat cgaggatcat caacacatcc agcagtggtt tcatacacgc
     8221 ccccttgcag tgtatgcggc gtcctgctgg cgtgatcgtt tactccagcg gcattaccag
     8281 cggatcggga tactggtatt caaatcccag ttcattacaa atccgactgc catcaataat
     8341 cttacctttg ccgctgtcca gactatttct gaactgcgga ggctccagcc ccagtaaacg
     8401 ggccatctgc ggatagaaaa cattacgcgc agggtgagct ggcgcacata tattatagat
     8461 gtgtccgcct ttaggtgcct gtaacaacag agtgatagcg ccaataacat cttctaaatg
     8521 gactaaatta acaccatgtt caccatcagg cgcggttttt ccggcaaaga agcgtccggg
     8581 atgacgtccc ggtccgacca gccccgcaag acgcagaata tcgaccgaag taccgggtaa
     8641 attgtgcagc cagtcttcga gttcttctaa tactcgtcca ctgttggtta ccggattacg
     8701 cggggtagtt tctttcaccg tgccttgcgc gtcgccatag acagatgttg agctggtaaa
     8761 aataatgcgg ggaatacgat gggccagcgc gctatccact aactcttgta ccgcttgtaa
     8821 atagaactca tcgccggggc cgctacgacg tgccggaagc gtaatgacca gcgcatcggc
     8881 atccatcagg gcatccagat cgtcagaatc gcaaactagc tcaggctcca tgcgaagcag
     8941 atagctatca atgccactca ttcgggccgc ttcgacacca tcttgtgtgg ttttactccc
     9001 agtgacttgc cagcctcgcg ctgaaagtga catcgccagc ggcatgccta accaccctaa
     9061 cccgacaatt gcgacctttt tcatccgtta tctcctgata caccttagat ctataaggct
     9121 acgctagcgt atcaaaactg acaattcatt ctatgaatga atctgttcaa taatgataac
     9181 gacatgctgc aatgagcagt gaatcgtcgg taaccgcgta taccagacgg tgctcctctg
     9241 taatgcgtcg ggaccagaaa cctgacaaat tatgtttcag gggttctggc ttccccttac
     9301 cttcaaatgg cgttctgcgg gtatctttga taagttcatt gatcttttta acaattcgct
     9361 tatctgtttc ctgccagtac agataatcat cccatgattc ctcagaccag attagtttca
     9421 ctcaataatg tccttttctg ttcctttgcc tgatttcagg ctatcgattg agtccatcaa
     9481 tctccgggcg ttagcggggg aacgcagtag ataagccgtc tcttccagcg agttgtattc
     9541 ttcgagtgac atcagaacac aagcctctcc attctgacga gtaataagga tcggggcatg
     9601 atcttcaacg gctttcatca ttgttgccga caaattctga cgcgcttcgc tgtagctaat
     9661 tgtacgcatg tcaatctcct cttttgtaca gttcattgta caatgatggg cgttaattaa
     9721 ctatttatta attagtttgt agatcaaggt attgtcagtg agacgaaaat ccaggcttcg
     9781 ctatttttgg tgccgtcagc taagaggaca gtcctcttag ccccctcctt tccccgctca
     9841 ttcattaaac aaatccatcg ccataaaata tataaaaaag cccttgcttt ctaacgtgaa
     9901 agtggtttag gttaaaagac atcagttgaa taaacattca cagagacttt tatgacacgc
     9961 gttcaattta aacaccacca tcatcaccat catcctgact agtctttcag gcgatgtgtg
    10021 ctggaagaca ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc
    10081 accttccggg ggctttttta ttgcgcggtt gataacggtt cagacaggtt taaagaggaa
    10141 taacaaaatg acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag
    10201 tgatgactca cgtgaattgc tggcgcgctg tggcattaaa atcaatcttc acacccagcg
    10261 cctgatcgcg atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgacgacat
    10321 tcccggtctg gtaatggacg gcgtggtaga tcttgggatt atcggcgaaa acgtgctgga
    10381 agaagagctg cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccttgcgtcg
    10441 tctggatttc ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggacgg
    10501 cccgctctcc ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta
    10561 tctcgaccag aaaggcatct cttttaaatc ctgcttactg aacggttctg tcgaagttgc
    10621 cccgcgtgcc ggactggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga
    10681 agctaacggc ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg
    10741 cgatggcgaa atggaagaat ccaaacagca actgatcgac aaactgctga cccgtattca
    10801 gggtgtgatc caggcacgcg aatcaaaata catcatgatg cacgcaccga ccgaacgtct
    10861 ggatgaagtc atcgccctgc tgccaggcgc cgaacgccca actattctgc cgctggcagg
    10921 tgatcaacag cgtgtagcga tgcacatggt cagtagcgaa accctgttct gggaaacgat
    10981 ggaaaaactg aaagcgctgg gtgccagttc aatcctggtc ctgccgattg agaagatgat
    11041 ggagtgatcg ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa
    11101 caacgccagc tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt
    11161 aacgatattc tcgataacgt gaaaacgcgt ggcgatgagg ccctgcggga atacagcgcg
    11221 aagtttgata aaaccacggt taccgcgctg aaggtgtctg ctgaggagat cgccgccgcc
    11281 agcgaacgcc tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gaatattgaa
    11341 accttccaca ctgcgcaaaa actgccgcca gtagatgtag aaactcagcc aggcgtacgt
    11401 tgtcagcaag tcacgcgccc ggtggcttcg gtaggtttgt atattcctgg cggctccgcc
    11461 ccgctcttct caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa
    11521 gtggtgctgt gctcaccgcc gccgattgcc gatgagatcc tttgtgcggc gcagctgtgc
    11581 ggtgtgcagg acgtgtttaa cgtcggcggc gcacaggcca ttgccgcact ggcgtttggt
    11641 acggaatctg tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa
    11701 gcgaaacgtc aggtgagcca gcgtctggac ggtgcggcta tcgatatgcc cgcaggcccg
    11761 tcggaagtgt tggtcattgc cgacagtggc gcaacgccgg atttcgtggc ttctgatttg
    11821 ctttctcagg ctgaacacgg cccggactca caggtgattt tactgacgcc cgatgctgat
    11881 atggcgcatc aagttgccga ggccgtcgaa cgccagttgg cagaactgcc gcgtgccgaa
    11941 accgcccgtc aggcacttag cgccagccgc cagatcgtga ctaaagattt agcgcagtgc
    12001 gtagagatct ccaaccagta cggcccggag cacctgatca ttcagacccg caacgcccgt
    12061 gaactggtcg atagcattac cagtgccggt tcggtatttc ttggtgactg gtcaccggaa
    12121 tcggcaggtg attacgcctc cggcaccaac cacgttctac cgacttacgg ttacaccgcc
    12181 acctgttcca gcctcgggct ggcagatttc cagaagcgca tgaccgtgca ggaactgtcg
    12241 aaagaaggct tttctgcgct ggcttcgacc atagaaacac tggccgccgc cgagcgcctg
    12301 accgcccata aaaatgccgt tactttgcgt gttaacgccc ttaaggagca agcatgagca
    12361 ccgtgactat taccgattta gcgcgtgaaa acgtccgcaa cctgacgccg tatcagtcgg
    12421 cgcgtcgtct gggcggtaat ggcgacgtct ggctgaacgc caacgaatat cccacagccg
    12481 tggagtttca gcttactcag caaacgctca accgctaccc ggaatgtcag ccgaaagcag
    12541 tgattgaaaa ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg
    12601 gcgcggacga aggtattgaa ctactgattc gcgctttttg cgaaccgggt aaagacgcca
    12661 tcctctactg cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggcgtcg
    12721 agtgccgcac agtgccgacg ctggaaaact ggcaactgga cttacagggc atttccgaca
    12781 agctggacgg cgtaaaagtg gtctatgttt gcagccccaa caacccgacc gggcaactga
    12841 tcaacccgca ggattttcgc accctgctgg agttaacgcg cggtaaggcg attgtggttg
    12901 ccgatgaagc ctatatagag ttttgtccac aggcatcgct ggctggctgg ctggcggaat
    12961 atccgcacct ggctattttg cgcacactgt cgaaagcttt tgctctggcg gggcttcgtt
    13021 gcggatttac gctggcaaac gaagaggtca tcaacctgtt aatgaaagtg atcgccccct
    13081 acccgctctc gacgccggtt gccgacattg cggcccaggc gttaagcccg caggggatcg
    13141 tcgccatgcg tgagcgggtg gcgcaaatta ttgcagaacg cgaatacctg attgccgcac
    13201 tgaaagagat cccctgcgtg gagcaggttt tcgactctga aaccaactac attctggcgc
    13261 gctttaaagc ctccagtgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg
    13321 atcagaataa acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag
    13381 aaagccagcg cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct
    13441 ttttatcgat cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg
    13501 ttttgataaa ctcgcctttg aaccgggcgt gatcccggag ctgatgaagc tgcaaaaagc
    13561 gggctacaag ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccgca
    13621 ggcggatttt gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca
    13681 gtttgatgaa gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc
    13741 gaaagtaaaa ctggtggagc gttaccttgc tgagcaagcg atggatcgtg ccaacagtta
    13801 tgtgattggc gatcgcgcga ccgatattca actcgctgaa aacatgggca ttactggttt
    13861 acgctacgac cgcgaaaccc tgaactggcc gatgattggt gagcaactca ctaaacgaga
    13921 ccgttacgcc catgtagtgc gcaacaccaa agagacgcag attgacgttc aggtgtggct
    13981 ggatcgcgaa ggtggcagca agattaacac cggtgttggc ttctttgatc acatgctgga
    14041 tcagatcgcc acccacggcg gtttccgtat ggaaatcaac gtcaaaggcg acctctatat
    14101 cgacgatcac cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc
    14161 cctcggcgat aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct
    14221 tgcccgctgt gcactggata tctctggtcg cccgcacctg gaatataaag ccgaatttac
    14281 ctaccagcgc gtgggcgatc tcagcaccga aatgatcgag cacttcttcc gttcgctctc
    14341 atacaccatg ggcgtgacgc tacacctgaa aaccaaaggt aaaaacgatc accaccgtgt
    14401 agagagcctg ttcaaagcct ttggtcgcac cctgcgccag gccatccgcg tggaaggcga
    14461 caccctgccc tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga taccggctgc
    14521 gccaatctga actcggtgaa gtcagccatt gcgcgccacg gttatgaacc aaaagtcagc
    14581 cgtgacccgg acgtcgtgtt gctggccgat aaactatttt tacccggcgt tggcactgcg
    14641 caagcagcga tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc
    14701 caaccggtac tgggcatctg cttagggatg caactgctgg gtcggcgcag cgaagagagc
    14761 aacggcgtcg acttgctggg catcattgac gaagacgtgc cgaaaatgac cgactttggt
    14821 ctgccactgc cgcatatggg ctggaaccgc gtttacccgc aggcaggcaa ccgcctgttt
    14881 caggggattg aagacggcgc gtacttttac tttgttcaca gttacgcaat gccggtcaat
    14941 ccgtggacca ttgcccaatg taattacggc gaaccgttca ccgcggcggt acacaaagat
    15001 aacttctacg gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg
    15061 aaaaacttcc tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg
    15121 tgcgtctcca tcagggcgat tacggcaaac agcgcgatta cggtaacgac ccgctgccgc
    15181 gcttacagga ttacgccgcg cagggtgccg aagtgttgca tctggtggat ctgaccgggg
    15241 caaaagatcc ggctaaacgt caaatcccgc tgattaaaac cctggtggcg ggcgttaacg
    15301 ttccggtgca ggttggcggc ggcgtgcgta gcgaagaaga cgtggcggcg ttactggaag
    15361 cgggcgttgc gcgcgtggtg gtcggctcca ccgcggtgaa atcaccagaa atggtgaaag
    15421 gctggtttga acgtttcggt gccgatgcct tagtgctggc gctggatgtc cgtattgacg
    15481 agcaaggcaa caagcaggtg gcagtcagcg gctggcaaga gaactcgggc gtttcactgg
    15541 aacaactggt ggaaacctat ttgcccgtcg gcctgaaaca tgtgctgtgt accgatatct
    15601 cgcgcgacgg cacgctggca ggctctaacg tctctttata tgaagaagtg tgcgccagat
    15661 atccgcaggt ggcatttcag tcctccggcg gtattggcga cattgatgat gtcgctgccc
    15721 tgcgtggtac tggtgtacgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca
    15781 ccgtgaagga ggccatcgca tgctggcaaa acgcataatc ccatgtctcg acgttcgtga
    15841 tggtcaggtg gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc
    15901 gctggcaaaa cgctacgccg aggaaggtgc agacgaactg gtgttctacg atatcaccgc
    15961 ttccagcgat ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga
    16021 cattccgttt tgtgtggcgg gtgggattaa gtctctggat gacgccgcca aaattctttc
    16081 ctttggcgcg gataaaatct ccattaactc ccctgcgctt gcggacccga cgttaattac
    16141 tcacctggcg gatcgctttg gcgtgcagtg tattgtggta ggtattgata cctggtacga
    16201 cgctgaaacc ggtaaatatc atgtgaatca atataccggc gatgaaagcc gcacccgcgt
    16261 cactcaatgg gaaacgctcg actgggtaca ggaagtgcaa aaacgcggtg ccggagaaat
    16321 cgtcctcaac atgatgaatc aggacggcgt gcgtaacggt tacgacctcg aacaactgaa
    16381 aaaagtgcgt gaagtttgcc acgtcccgct gattgcttcc ggtggcgcgg gcaccatgga
    16441 acacttcctc gaagccttcc gcgatgccga cgttgacggc gcgctggcag cttccgtatt
    16501 ccacaaacaa ataatcaata ttggtgaatt aaaagcgtac ctggcaacac agggcgtgga
    16561 gatcaggata tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacgga
    16621 ctgatgccgg tgattgtgca acacgcggta tccggcgaag tgttaatgct gggctatatg
    16681 aacccggaag ccttagacaa aaccatcgaa agcggcaaag tcaccttctt ctcgcgcact
    16741 aaacagcgac tgtggaccaa aggcgaaacg tcgggcaatt tcctcaatgt ggtgagtatt
    16801 gccccggact gcgacaacga cacgttactg gtgctggcaa atccaatcgg gccaacctgc
    16861 cacaaaggca ccagcagctg cttcggcgac accgctcacc agtggctgtt cctgtatcaa
    16921 ctggaacaac tgctcgccga acgtaaatct gccgacccgg aaacctccta caccgccaaa
    16981 ctgtatgcca gcggcaccaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc
    17041 gcactggccg cgactgtaca tgaccgcttt gagctgacca acgaggcgtc tgatttgatg
    17101 tatcacctgt tggtattgtt gcaggatcag gatctggatt taacgacggt gattgagaac
    17161 ctgcgtaaac ggcatcagtg agttgcgggg taagcggatg cgatattgtt gtcgcatccg
    17221 gcaaaaaaaa acgggcaagg tgtcaccacc ctgcccattt tctttaaaac cgaaaagatt
    17281 acttcgcgtt gtaattacgc agagcgttac gccccaacac aatccccgcg ccaaccatgc
    17341 cgcccagcag caccgccaga atcaaggtaa ttgccttttt cgggctatcg cgacgaatag
    17401 gtaacgtcgg tttcattaca tagcggtaag catgaatatc aagtttatca acgtcaagat
    17461 tgtcgatatc cagcaggttt tgacgagtct gatagtagtt tgatgagaac accaacggac
    17521 gagtcgcctc gtgcttaatc atcgactcca gcgcttcgct ccccaaaagg aacatagtat
    17581 cctgggttac gtcctgtgtc tgctgaatct gcggctttgt cacctgcgcc tgattcgcat
    17641 actgcaacgc ttcctgaatc tgacggatac gcagatcttt ctgctcctgc gcaaccactt
    17701 cctgggtctt cagagaatcc tgcaagtttt tcatttgcag cgcgatgttg tctttcaggt
    17761 cttgttccag ttcttcattt acctggtcgt caacttgctg gatatactgc gccagctgtt
    17821 tctgcgcacc ttcaggtgtt tcacccacat aagaaaccgc taaaggtaat gactgatttt
    17881 taacggtagg ctcaatggtc agtttttcag gttcttcctg attatccagc gtttctgcta
    17941 atgcagaaaa tgtcgtgctg aaacgaccaa ttaacatcga ctggatctca gacactttcg
    18001 gcgctgccga accataaatc acatttatgg cattgttata accagcgatt tgtcccacat
    18061 caggttgagt aacgatagct gtagaagtcc acttttcttt ggcaacggcc agatacccta
    18121 tagccagggc aatagcgaca atgacagaga tgataattgt catcttgcca cgccacaact
    18181 gcaccagtaa atcaatcaaa tcaatctgtt ccgggtcatg gttttgccca gaaacattat
    18241 tattttctac tctcatcatt accctaacgg acgaaataat tttaagtcgc catagtttag
    18301 cgataatcgc catgaatgct ataggataaa tgataaaaat aatgaattat tacaaaggga
    18361 gcataagaga atcagggcga aaatcgccct gataacagga tgttagtcac tgccaaagag
    18421 atcgcgggtg tagactttgt ctgccacatc cttaagctct tctgccatac ggttggagat
    18481 aatgacgtcg gcttgttgtt tgaaggtggc gagatcacgt tccaggcgag agttgaagaa
    18541 tgagtcttct ttcatcactg gctcgtatat gatcacttca acgcctttcg ccttgatacg
    18601 tttcataatc ccctgaatgg aagacgcacg gaagttatct gatccgctct tcataatcag
    18661 acgataaata cccactactt gcggcttgcg tgacaaaatg gcatcggcaa taaaatcttt
    18721 acgtgtacgg tttgcatcga caattgccga aatcaggtta ttcggcacag actggtagtt
    18781 cgccagtaac tgcttggtat ctttcggcag acaataacca ccataaccaa acgacggatt
    18841 gttgtaatgg ttgccaattc gcggatcgag acaaacgcct tcgattattt gacgggaatt
    18901 cagacctaaa ctttctgcgt agctatccag ttcgttaaag tacgccacgc gcatcgccag
    18961 gtaggtgttt gcaaaaagtt taatcgcttc tgcttcagtg gagtcggtaa acagggtagg
    19021 gatattttgc ttaatcgctc cttcctgtaa taacgcagcg aaacgttctg cgcgttctga
    19081 acgctcacca atgacaatac gtgaaggatg gagattatcg taaagggctt taccttcacg
    19141 gagaaattcc ggagagaaaa taaggttatc gatacccaga cgttctttaa ttgatttggt
    19201 gaaacctacc ggaattgtag atttaatcac catgacagca ttaggattaa tttctgtcac
    19261 atcgcgaatg accgcttcca cgcttgaggt attaaaataa tttgttttcg ggtcataatc
    19321 ggtaggcgtg gcaataataa cgtaatcggc atttttatac gcgtcatact tatctgtcgt
    19381 agcgcggaaa ttgagatctt tagtcgccag atactcttca atctccttat caacaatcgg
    19441 tgactgcttc ttgttaagca tgtccacttt ggcctgaacg atatccagtg caaccacttc
    19501 gtgattttgc gcaatcagaa taccatttga aagaccaaca taacctgttc ctgaaattgt
    19561 tattttcatt agctctgact tcttccggtt aaacatttag agtggtcatt aatccaccac
    19621 cacaacttca tctactgcgg gatttttaac gctgatgtag atgaactgtc aaggcagcga
    19681 tcctgctgtg cggcgctgta ttatatcgcg tttttaacta taaattataa aaaaaggcca
    19741 taacatgccg ctttgtataa taaaaaagcc cggagggttt ctccgggcct tgcattgatt
    19801 aattgattta aatcagatta atccagccat tcggtatgga acacaccttc tttatcaatg
    19861 cgcttataag tatgcgcacc gaaatagtca cgctgtgcct ggatcaggtt cgcaggcaga
    19921 acagcggcgc ggtagctgtc gtaataggca accgcagcag cgaaggtagg aacagggata
    19981 ccgttctgta ctgcataagc aacgacatca cgcagcgcct gctggtagtc atcggcaatt
    20041 tgcttgaagt acggagccag cagcaggtta gcgatctgcg gattttcggc ataagcatcg
    20101 gtgattttct gcaggaactg cgcacggatg atgcagccag cacggaaaat cttcgcgatt
    20161 tcgccgtagt tcagatccca gttgtactct tcagacgccg cacgcagttg agagaacccc
    20221 tgagcgtaag aaacgatttt gcccagatac agtgcacggc gaactttttc gatgaactca
    20281 gccttgtcgc cagctagctg cgcttgtggg ccagagagaa ctttagacgc agcaacgcgc
    20341 tgatctttca gagaagagat gtatcgtgca aacacagact cggtaatcag cgacagcggt
    20401 tcgccgagat ccagtgcgct ctggctggtc catttgcccg tacctttgtt tgccgcttca
    20461 tccaggatca catcaaccaa gtagttaccg tcttcatctt ttttagtgaa gatgtctttg
    20521 gtaatgtcga tcaggtagct gctcagttca ccgttattcc actcggtaaa ggtctgcgcc
    20581 agttcttcgt tggtgaggtt cagaccacct ttaagcagag aataggcttc agcaatcagc
    20641 tgcatatcgc cgtactcaat accgttgtga accatcttca catagtgacc tgcgccatcg
    20701 gcaccaatat aggtaacgca cggttcgctg tcttcagcca ctgcggcgat ttttgtcagg
    20761 atcggcgcaa ccagttcata ggcttctttc tgcccaccag gcataatgga aggacctttc
    20821 agcgcgcctt cttcaccacc ggagacaccg gtaccaatga agttaaagcc ttctgcagaa
    20881 agctcacggt tacgacggat ggtgtcctgg aagaaggtgt taccaccatc aatgatgatg
    20941 tcgcctttat cgaggtatgg cttgagagaa tcaatagcag catccgtgcc tgcacctgct
    21001 ttcaccatta acaggatgcg acgaggcgtt tccagagatt caacaaactc tttcaccgta
    21061 tagtaaggaa ccagtttctt gcctggattt tcggcaatca cttcttccgt tttctcacgg
    21121 gaacggttga aaatagagac ggtataacca cggctttcga tgttgagcgc aaggttgcgc
    21181 cccatcactg ccataccgac gacgccgatc tgttgctttg acattgttta ctcctgtcag
    21241 ggggtgttca ccgcagggcg gttatggata gtaataaact tattaattca tagtttacaa
    21301 aaactatttt ttattagtag cagatatgcc gaccgttttc aaataagtaa aaattgagta
    21361 ataaaaaatc atggagaatg ctagattaaa aaaaattggg cgttgatagt aagacagaat
    21421 gaacaaacaa ataaaaacga atgatgcctt caaaaatttg cacgcatacc cccccataat
    21481 tgggatcata tattgcataa tttgtaataa agcgat
//
LOCUS       DAZGAN020000079        21199 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGAN020000079 DAZGAN020000000
VERSION     DAZGAN020000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.079"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            291..791
                     /gene="tssB"
                     /locus_tag="ACHKYO_RS16405"
                     /old_locus_tag="pgaptmp_003281"
     CDS             291..791
                     /gene="tssB"
                     /locus_tag="ACHKYO_RS16405"
                     /old_locus_tag="pgaptmp_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HGU4430074.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            826..1050
                     /locus_tag="ACHKYO_RS16410"
                     /old_locus_tag="pgaptmp_003282"
     CDS             826..1050
                     /locus_tag="ACHKYO_RS16410"
                     /old_locus_tag="pgaptmp_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430075.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1101..2576
                     /gene="tssC"
                     /locus_tag="ACHKYO_RS16415"
                     /old_locus_tag="pgaptmp_003283"
     CDS             1101..2576
                     /gene="tssC"
                     /locus_tag="ACHKYO_RS16415"
                     /old_locus_tag="pgaptmp_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HGU4430076.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2583..2996
                     /gene="tssE"
                     /locus_tag="ACHKYO_RS16420"
                     /old_locus_tag="pgaptmp_003284"
     CDS             2583..2996
                     /gene="tssE"
                     /locus_tag="ACHKYO_RS16420"
                     /old_locus_tag="pgaptmp_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HGU4430077.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            3000..4850
                     /gene="tssF"
                     /locus_tag="ACHKYO_RS16425"
                     /old_locus_tag="pgaptmp_003285"
     CDS             3000..4850
                     /gene="tssF"
                     /locus_tag="ACHKYO_RS16425"
                     /old_locus_tag="pgaptmp_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGU4430078.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4814..5896
                     /gene="tssG"
                     /locus_tag="ACHKYO_RS16430"
                     /old_locus_tag="pgaptmp_003286"
     CDS             4814..5896
                     /gene="tssG"
                     /locus_tag="ACHKYO_RS16430"
                     /old_locus_tag="pgaptmp_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HGU4430079.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5921..7201
                     /gene="tagH"
                     /locus_tag="ACHKYO_RS16435"
                     /old_locus_tag="pgaptmp_003287"
     CDS             5921..7201
                     /gene="tagH"
                     /locus_tag="ACHKYO_RS16435"
                     /old_locus_tag="pgaptmp_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HGU4430080.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFMQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7198..7722
                     /gene="tssJ"
                     /locus_tag="ACHKYO_RS16440"
                     /old_locus_tag="pgaptmp_003288"
     CDS             7198..7722
                     /gene="tssJ"
                     /locus_tag="ACHKYO_RS16440"
                     /old_locus_tag="pgaptmp_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HGU4430081.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7725..9056
                     /gene="tssK"
                     /locus_tag="ACHKYO_RS16445"
                     /old_locus_tag="pgaptmp_003289"
     CDS             7725..9056
                     /gene="tssK"
                     /locus_tag="ACHKYO_RS16445"
                     /old_locus_tag="pgaptmp_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGU4430082.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9061..9822
                     /gene="icmH"
                     /locus_tag="ACHKYO_RS16450"
                     /old_locus_tag="pgaptmp_003290"
     CDS             9061..9822
                     /gene="icmH"
                     /locus_tag="ACHKYO_RS16450"
                     /old_locus_tag="pgaptmp_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HGU4430083.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9831..12596
                     /gene="tssH"
                     /locus_tag="ACHKYO_RS16455"
                     /old_locus_tag="pgaptmp_003291"
                     /gene_synonym="clpV"
     CDS             9831..12596
                     /gene="tssH"
                     /locus_tag="ACHKYO_RS16455"
                     /old_locus_tag="pgaptmp_003291"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HGU4430084.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESEQDNTGAVPADEADREQPEETAETVSPVQRLAHLTAELD
                     ALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQDLA
                     IASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINM
                     SEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQ
                     AFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPA
                     LLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVTDEIMSRVTRA
                     ENGARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGAFTADVEDAQNDESV
                     TKDETVL"
     gene            12593..13336
                     /gene="vasI"
                     /locus_tag="ACHKYO_RS16460"
                     /old_locus_tag="pgaptmp_003292"
     CDS             12593..13336
                     /gene="vasI"
                     /locus_tag="ACHKYO_RS16460"
                     /old_locus_tag="pgaptmp_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HGU4430085.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13341..14753
                     /gene="tssA"
                     /locus_tag="ACHKYO_RS16465"
                     /old_locus_tag="pgaptmp_003293"
     CDS             13341..14753
                     /gene="tssA"
                     /locus_tag="ACHKYO_RS16465"
                     /old_locus_tag="pgaptmp_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HGU4430086.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14862..18296
                     /gene="tssM"
                     /locus_tag="ACHKYO_RS16470"
                     /old_locus_tag="pgaptmp_003294"
     CDS             14862..18296
                     /gene="tssM"
                     /locus_tag="ACHKYO_RS16470"
                     /old_locus_tag="pgaptmp_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HGU4430087.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QALRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKELNAAPPESEEKLAVLRVMRMLEDKSGRNNQ
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18307..19756
                     /locus_tag="ACHKYO_RS16475"
                     /old_locus_tag="pgaptmp_003295"
                     /pseudo
     CDS             18307..19756
                     /locus_tag="ACHKYO_RS16475"
                     /old_locus_tag="pgaptmp_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19683..20165
                     /locus_tag="ACHKYO_RS16480"
                     /old_locus_tag="pgaptmp_003296"
     CDS             19683..20165
                     /locus_tag="ACHKYO_RS16480"
                     /old_locus_tag="pgaptmp_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGU4430088.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            20209..21123
                     /locus_tag="ACHKYO_RS16485"
                     /old_locus_tag="pgaptmp_003297"
     CDS             20209..21123
                     /locus_tag="ACHKYO_RS16485"
                     /old_locus_tag="pgaptmp_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430089.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLTSFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            21133..>21199
                     /locus_tag="ACHKYO_RS16490"
                     /old_locus_tag="pgaptmp_003298"
     CDS             21133..>21199
                     /locus_tag="ACHKYO_RS16490"
                     /old_locus_tag="pgaptmp_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430090.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFP"
BASE COUNT         5087 a         5709 c         5777 g         4626 t
ORIGIN      
        1 ttattatata attcaataag ttacataaaa aatctatatt taaattattg ccataataca
       61 cctgacgcga attgcacagg ttcacacaat ctatttaacg cttgcagaat acccctcttt
      121 acgatcgata aaaagtcctt ttcttattta ttttgaagcg taaaatgcgg ggtgatgaag
      181 gagtggtgat tttattttca ccgccattag ggcggcattg ccgatatttt tttgcggtac
      241 aaatatgccg tgttcatggc ctgtacgaga taacatgacg gatcactttt atgagcaaaa
      301 aatttgaagg cagcgtcgcg cctcgcgagc gtattaatat atcgtatgtc ccaaaaacag
      361 atggccagac ggctgaagtg gaacttccgc ttaacatgct cgttgtcggc gataccggaa
      421 atacgcagga aacatcccca ctggatgaac gtcaggcagt ttccgttaac aagcacaatt
      481 tcggtgcagt gatggctgaa gcagccattg gcctcaattt tactgtcccc gccacgctga
      541 agggaagcac aacagacgat gaactgaatg tggcgctgaa tatcaaatca ctggatgatt
      601 tttcaccaga tagcgtggcc cggcaggttc cggaagtgaa taaattgctg gaactgcgtg
      661 aagcactgac tgcactgaag gggccgatgg gtaaccttcc ggcattccgt acccagctcc
      721 aggcattgct ggaaaatgaa gagtcccgcg agcagttgct caaagaaatc ggtctggtga
      781 gcaataaata accaccacta tttcattaaa cagggttatc caggcatgac ggcacacaaa
      841 aatatctccg ggacatacca cctctctgtt gcagacattc tgcaggtggt ttaccaggta
      901 tgtttcagcc cgtccgttga aatcaaccag gatggtgtgg ctgcgctgat tacaacactg
      961 gatcgccgta tcagtgatct attggatgag attatccatt tttgtgagtt tcagcagagc
     1021 gcgtcgcact ggcagcgcgt acttcactga cggcgaaaag ccggtttcag aaacacagct
     1081 aaaacagaag gattttcagt atgtctttac aggaagagga actcgtatcc agtcatgccg
     1141 ggcagcctga gcaggcatcg tcgctgcttg atcaaatcat ggcacagacc cgtattcagc
     1201 ccggttcgga aggttatgac gttgcccgcc agggtgtgac agcctttatt gccagcatcc
     1261 tgcaatccac tgcatctgcc gaaccggtca ataaactggc cgtggacagc atgattgcag
     1321 atattgatga acgcatcagc cgccagatgg atgtcatcat ccatgcgcct gcctttcagc
     1381 aggtcgagtc gttctggcgc tcactgaaaa ccatggtgga tcgcgttgat ttccgtgaaa
     1441 acatcaaggt caacgtgctt catgtcacca agcaggaact tctggaagac tttgagtttg
     1501 cgccagaaat tattcagtcc ggattttata agcacgttta ttcgtccggt ttcggacagt
     1561 ttggcggtga acccatcgct gccgtgctgg gcgcttatga gttcaaaaat accgcgcctg
     1621 acatgaaact gctgcaatac gtcagtgccg tgggcgcaat ggcgcacgca ccgttcctgt
     1681 cctccgtttc cccggagttt atggggctga actcgtggac cgaactgcct aatatcaaag
     1741 atctttatgc catctttgaa ggtccggcct acaccaaatg gcgcgccctg cgtgactcgg
     1801 aagattcgcg ctatttaggg cttacggcac cccgcttcct gcttcgccag ccctattcac
     1861 caacagataa ccctgttaag aactttaact attacgaaga tgtcagccag aaccacgaag
     1921 attatctgtg gggtaatacg gcctggatgc tggcatgcaa tatcgcagac agttttgcca
     1981 aataccgctg gtgtccaaac attatcggtc cgcaaagcgg cggcgcagtg aaagatctgc
     2041 cggtgcatct gttcgaaacg atggggcaga ttcaggccaa aatcccaacc gaggtgctgg
     2101 tcaccgatcg ccgcgaattt gaactggctg aagagggttt tatcaccctt accatgcgta
     2161 aagactctga taatgcagcc tttttctctg caaactcggt acaaaaaccg aagcactttc
     2221 cgggaaaaga tgcagaaacc aactataagc tgggtacgca gcttccttat ctcttcatca
     2281 tcaatcggtt agcgcactac atcaaagtgt tgcagcgtga acaactgggg tcatggaaag
     2341 aaaggagcga cttagaaaga gagcttaata cctggatccg tcagtatgtt gccgaccagg
     2401 aaaacccgcc tgcagacgtg cgcagccgca aacctctgcg cgctgccaaa gttgaggtta
     2461 tggatgtaga aggcgaaccc ggctggtacc aggttgcgct aagcgtgagg cctcatttta
     2521 aatttatggg ggcaaatttt gagctttccc tggttggccg gttagacagg gagtaacaca
     2581 ccatgatacc ggatcgggaa agaaccacag gttctctttt tgagcgtatg gaagcctctt
     2641 cggccagaaa ccggcaggga ggcagtattc attcgctcag gcaatccatt cgccagaacc
     2701 tacgaaacat tcttaatacc cggtccggta gttgccgggg tgcgcctgag ctgggcattg
     2761 atgaaccgga aggggctgaa aacttccgcg aatccatgag tcgggccatc gagcaatgta
     2821 tagaacgcta tgaaccccgg atctcacacg ctgaagtgca ggcagtggtt tcatccgcct
     2881 ccagcccgct ggatatgacg tttcatatta cagcctgggt gacatttaac gaaacgcatg
     2941 aggtgcttga atttgatatg gcaccgaatg gcagccagca ttaccgggtg gactgacaaa
     3001 tggaatttga agaacgctat ttccgggaag aactcgatta cctgcgccag cttagcaagc
     3061 tgctggcaac ggaaaaaccc catctggccc gcttcctggc cgaaaaagat gcggatccgg
     3121 atattgaacg cctgctggaa ggggtggctt ttcttaccgg caatctccgc cagaaaattg
     3181 aggatgaatt cccagaactg acgcacgggc ttattaagat gctatggcct aattacctgc
     3241 gtccggttcc ggcaatgacc cttattgaat atacgccgga tatggataag tcttctgtac
     3301 cggtgttaat cccccgtaat gagcagttta caaccaacgc cggggaaatc agagttgatg
     3361 aagtgctgcc ctctgatgct aaaaaggagg agccgcctcc ctgtaccttc actctctgcc
     3421 gggatatctg gctgctgccc gttcgcctgg agcagattga aaaccgcagt acgacccgta
     3481 atggtgttat caacatcacc ttttcggtcg caccgggaac ggacttccgc acgctggatc
     3541 tgaacaaact tcgcttctgg ctcggcaatg acgacaacta tacccgtgac cagctttatt
     3601 tatggttctg cgaatacttg cagggtgccg acctgactgt gggtgaacag catattcgcc
     3661 tgcctgagtt tatgctaaaa gctgtcggtt ttgagccgca ggatgccatg ctgccctggc
     3721 cgaaaaacgt ccacagcggc taccggatcc ttcaggagta tttctgttac cccgatgcgt
     3781 ttctcttttt tgatctttgt ggttgtccgg ctttgcctga cggattgcag gcggaattct
     3841 ttaccctgca actgcgtttt tcgcgccctt tgcccgtgga catccggctg cgccgcgatt
     3901 ccctgcgcct gtattgcgca cctgccatta atttatttat ccaccatgca gaagccatca
     3961 cgctggacaa ccggcgggca gactatccgc tggttcccag ccgccattac ccacaacatt
     4021 acgatgtatt ttccgttaac agtgtggtga gccaggtcca ggatatgttc aggaaaaaag
     4081 atctggggcg tcctgtttcg acgcaggccg cgcgccagtg gccagccttt gaaagtttca
     4141 gccatcagat ggaatacagc cggaagcggg aagtggtgta ctggcatcac cggaccaaaa
     4201 catccctgtt ccatcgcggc tttgatcata cccttgcctt tatacatgct gatggcagtt
     4261 atccgtcaga cgaatctctg ctcagtaatg aagtggtttc ggtatcgctg acctgtacca
     4321 accgtgagct tccgtcacaa attcgttccg gcgatatcac cggcacaacc ggtaaaaatg
     4381 cggctgttgc ttcatttcgc aacattaccc gcccgacgca accactctgg ccggtcattg
     4441 atggcagcct gcactggtcc ctactctccg ccatgaacct gaattatctg tcattactgg
     4501 atacggacgc gctgaagcag gtcatcgcca actttgatcg ccacgcaatc catcatccgc
     4561 agacggcacg gctgtcacaa caaaagctgg atgccattga gcgtctggag acccgccccg
     4621 ttgatcgcct gtttacgggt attcccgtcc ggggactggc ctccacgctg tatctgcacc
     4681 cggagccgtt tgtctgtgaa ggggaaatgt atctgctcgg tacggtgctt tcgcattttc
     4741 tgtcgctgta cgccagcgtt aactcattcc acatgctgac cgttgtgaac acagaaagcc
     4801 aggagacatg gaaatggacg gaaagaatcg ggcagcatcc tcttatctga gtccgggaaa
     4861 tcccccggca gataaagagc agaatgatcc gctggcacag gttttccata atgcctgctc
     4921 ttacaatttt tttgcgatgg cggagctgtt gcaccgcctg gcaaagggtg aaaaggggac
     4981 gccagaatta tccctgcgtg acgatccggc acaggaaacc ctgcgtttca gcgccgatgc
     5041 cagccttgcc ttcccgtgca gtgatatcag tgcactgaaa cgggatacgt caggggcatt
     5101 caggatgacc accaccttta tggggttaca ggggagccag tcgcctctcc ccggttatta
     5161 cctcgatcac ctggcctgga aagcggtaca tgaacaaagt ccggtaggtg attttctgga
     5221 tatgttcagc caccgcctga cccagtttgt ctggcatatc tggcgtaagt accgctatca
     5281 catcagtttt cgtaacggcg gtgtggatgc gttctcgcag cgcatgtact cccttgtcgg
     5341 gctgggccac cgccagcttc gcgataagct ggcgattaat cacagcaaaa tgctggcgta
     5401 ttccgggatc ctggcaaatc cgggacgatc cccggaaata atctgtggcc tggtgtccca
     5461 ctgctttgat ttgtcagaag tgacgctgca aaactggcaa cgtcgcaagg tggatattga
     5521 accggatcag caaaatagtc tggggagcta ttctcttaaa aatggcgaaa aactggcggg
     5581 aagatcggta ctgggcaact ttgttctggg tacacgagtg cccgatctca gcggaaaatt
     5641 ccagctcagt attaccagcc tgacccgaaa acagttcctc tcttttctgc cgtccggcga
     5701 aaacttcctg ccactgacga tgttcgtgtc cttcatcctg cgcgatcagc ttgcctggga
     5761 cttacatctg gggctggcgc cggagcaggt gggcgcaatg cgcctgggcg ataacaaaag
     5821 tgcgctgctg ggctggacca gcttcctcgg cacaccggaa gaacgaccct cagtcaccat
     5881 cagggtacgg tcgtaaatta tgataatcac ggagcagaat atgccggaag agaaactaca
     5941 gacgctttca ttgcaggtca ttaacggcag tgagctggaa agcggacggg cggcgcgctg
     6001 tctgttcaca cagcagggaa atgtcggcca tggccccgaa tgccactggt cggtacagga
     6061 tcgtcagcag agcattccgg cccaggcttt taccgttatc ctgcacgatg gcacattttg
     6121 tctgcgcccg cagacggcac aactgtggct gaatcaggca aaagtcacag caacatcaga
     6181 cctaatacag ttgcgccagg gcgatgagat ccagatcgga cggctgatgg tgagggttca
     6241 tctgaaccgg ggagatattc cccattacga tgaggaaatg gccactcccg aaaccatcgt
     6301 taccaatcgc gatatgctca cggataccct gctatcaacg gagggtacgc cacactatcc
     6361 gggaatgact caccggcacc aacttgcaga caccgtggta aacggttttt ctgccgatcc
     6421 actccaggca cttcagtccg aaagcctgat taccacgggc gatccgcttt caggcattgc
     6481 ggctgtccgg ccatcggcac cgctgtccga tccggcaagt aatgggggga tcaatactcc
     6541 gtttatggat ctgccgccca tttatgccag ccccggcgat cgtaatgacg acgtctctgc
     6601 ggcagaaatg gcgcaacgcc accttgcggt caccccctta ctgcgcggtc ttggcggctc
     6661 gcttaccatg agcaattccg acgatgcgga tgattttctg gaggaggccg gacgaacgtt
     6721 acaggccgca ataaaaggtc tgctcgattt gcagcagcag cgtaacagcc tctcagacaa
     6781 acatttgcgc ccgctggaag ataacccgct gcgcctgaac atggattacg ccaccgcgct
     6841 cgacgtaatg tttgccgaag gtaaaagccc ggtacatttg gcggctcccg ccgccgtcag
     6901 tgaaagcctg cgcaatgtcc gccaccacga agaagctaac cgggcagcga ttgtggagtc
     6961 gcttcgtgtc ctgctggatg ctttctcacc acaaaatctg ctgcgccgct ttatgcagta
     7021 ccgccgcagc catgaactgc gccagccgct ggatgatgcc ggagcatggc aaatgtacag
     7081 ccattattac gaagaactgg cctccgatcg ccagcagggg tttgagatgc tgtttaacga
     7141 ggtctacgcc caggtctatg accgggtgct tcgtgaaaaa cagcgggagc cggaagcatg
     7201 aacaggaagg cgtttctggc ctgcgtgctg atgtgcgtca tattaaccgg ctgcgaaacg
     7261 gcgaaaaaaa tcagtgaggt gatcaaaaac ccggatatcc aggtcgggag cctgaaggag
     7321 caaccttcgg aaataaccgt caccctgctg accgaaccgg acaccaatac caatgcggaa
     7381 ggtgaatcag ccgccgtgga tgtgcaactg gtgtacctga ctgacgactc aaaattgctg
     7441 gcagccgact atgaccagat agccagtacg ccgctaccgg atgtgctggg taaaaactat
     7501 atcgaccacc aggatttcaa cctgttgccg gacaccatca aaaccctgcc gccggtaaag
     7561 ctggatgaga aaacgcagtt tattggtgtc gtggcctatt tctccgatga ccaggccact
     7621 gaatggaaac aaatcgagac ggtggaagga accggccacc attaccgcct gctggtgcat
     7681 gtacgtcaaa gcagcatcga aatgaaaaaa gaggatgaat aattatggca accacccgca
     7741 acaaggtgat gtggcaggaa gggatgctga tgcgtccaca ccatttccag cagcagcagc
     7801 gttacaacga ctacctggat aaccagcgtt tccgggccat gaatgattta tcctggggat
     7861 ttaccgaact cactctcaac aatgaactgc tggcacaggg taagatcatg attgacagcg
     7921 cgtcaggcac actgcccgac ggcaccgtct tttctatccc cgaccaggac gcactgcccg
     7981 atccgctgca cccgcaacat tttccggacg agagaagccg caatatctac ctcgctctgc
     8041 cggtcgccag tgatgtgaga aatgaaatca gcgacgggcg gcgaatcggg cgttaccggc
     8101 tgaattatgc cgatgtccgg gatttgcatt cagaagaagg tgacacgcga acactgacgc
     8161 tgggacaact gacgccgcgc attatgagcg gtgcagaaga tatgagcgcc tatattacgc
     8221 tcccgctttg ccgtatcagc gatcgccatg ccgacggctc cctgacgctg gatgacgatt
     8281 ttatcccctc ctgccagaat attcaggtca gtaagaaact gcgtgtttat ctcaaagagg
     8341 tacagggggc cattggcgga cgggcaagcg atctggcaaa ccgcattggc tctccggcgc
     8401 agagcggcat cgcggatgtg gcggaattta tgatgttgca gttacttaac cgtaaccaga
     8461 cccggtttac ccatcacgcc cgtcgatccc agctccaccc ggaagatttc taccttgatc
     8521 ttgccgggtt gctgggtgaa ctgatgacct ttaccgagcc gtcgcgcctg ccctgcccgc
     8581 ttgatgtgta tgatcatcat gacctgacca aaacatttaa aacgctgtta ccggaagtca
     8641 aacgggcgct gcataccgta ctgtcgccaa gagccgtcaa tctgccgctg catctgcgtg
     8701 acggtatctg gcaggccgat atccatgaca cagaactgct gcaatctgcc acctttgtgc
     8761 tggccgtggc ggcaaacatg ccggtcgatc agatccagcg ccagtttatc cagcagtcga
     8821 aaatttcctc gccggaaaaa atccgcaata tggtcagtgt gcagatcccc ggcattccat
     8881 tgcgcgcact gatggtggct ccccgccagc ttccttacca ttccgggttc agctatttcg
     8941 aactcgacaa gagcggacag gcctggacag aaatggctgc cgccggggcc gttgcactgc
     9001 atgtatccgg cagtttcccg gatctgaaca tgcaactgtg ggcgataaga gggtaagacg
     9061 atggatgagg ggtcactgtc tctgccacca tttaccggat acgacgaaaa aagccagcgc
     9121 aactaccatc tcgcgcttcg tggcaacagc ctgaatccga tgatcgacgc ggccacgccg
     9181 ctactcggta tggtaatgcg cctttcaacc atgaacagcc agacgatgcc ggagcatctt
     9241 tttgctcagg tggtgacgga tgttcaggcc gtggagcaac tgcttcagga gcagggctac
     9301 gaaccgggcg tgattatctc gtttcgctac atcctctgca cgtttattga tgaagcggcg
     9361 ctcggtaacg gctggtcaaa caagaacgag tggatcaagc agtcattgct ggtccatttc
     9421 cacaacgaag cctggggtgg agagaaagtt ttcatcctgc tggagcgcct gatccgtgaa
     9481 cccaaacgtt accaggatct gctggaattt ctctggatct gtttttctct gggatttcgc
     9541 gggcgctaca aagtggcagc acaggatcag ggggaatttg agcagatcta ccgccgtctg
     9601 taccacgttc tgcataaact gcgcggcgat gcccctttcc cgctgttaca ccaggacaaa
     9661 aaaacccagg gcgggcgtta tcagctcatc agtcgcctga ctgttaaaca tattttttgt
     9721 ggtggcgttg tcgtgctggc gttgttttac ctgttctatc tgttgcggct ggacagccag
     9781 acgcaggaca tcctgcacca gctaaacaaa ttacttcgat aaggacgttt atgatccaga
     9841 ttgatcttcc cacgctggta aaacggctga acctgttctc ccgccaggcg ctggagatgg
     9901 ccgcctctga atgtatgagt cagcaggcag cggaaattac cgtcagccat gtgctcattc
     9961 agatgctcgc catgccacgc agtgacctgc gggttattac ccgccagggc gatattggca
    10021 tggaagagtt gcgccaggcg ctgacggtag agaactacac aaccgcccgt tctgcggaca
    10081 gctacccggc gttttccccg atgctggttg agtggctgaa agagggctgg ctgctggcgt
    10141 cggctgagat gcagcacagc gaactgcgcg gcggcgtgtt gctgctggcc ctgctgcatt
    10201 cgccgctgcg ttatataccg cctgctgccg cccggctgtt gaccggcatt aaccgtgacc
    10261 gtctgcaaca ggactttgtg cagtggacac aggagtcggc ggaatcagtc gtgccggatg
    10321 cagacggtaa aggcgcaggc acaatgacgg acgcctctga caccctgctt gcccgctatg
    10381 ccaaaaacat gaccgcagac gcccgtaacg gcaggcttga cccggtactg tgccgcgacc
    10441 acgaaatcga cctgatgatc gacattctct gccgccgccg taaaaacaac ccggtggtgg
    10501 tgggcgaagc gggcgtgggc aaaagcgcac tgattgaagg gctggcgctg cgcatcgtgg
    10561 caggccaggt gccggacaag ctgaaaaaca ccgatatcat gacccttgac ctgggcgcat
    10621 tgcaggccgg ggcgtcggtg aagggtgaat tcgaaaaacg tttcaaaggg ctgatggcgg
    10681 aggtcatttc ctccccggtg ccggtcattc tgtttatcga cgaagcacat accctgattg
    10741 gcgcgggcaa ccagcagggc gggctggata tctccaacct gctcaaaccg gcgctggcgc
    10801 gcggcgagct gaaaaccatc gccgccacca cctggagcga gtacaaaaaa tacttcgaaa
    10861 aagatgccgc cctgtcgcgc cgcttccagt tggtgaaggt cagcgaaccc aacgctgccg
    10921 aagccaccat tattctgcgc ggtctgtcgg cggtctatga acagtctcac ggcgtgctga
    10981 ttgatgatga cgccttgcag gccgctgcga cattaagcga gcgttatctc tccgggcgtc
    11041 agttaccgga caaagcgatt gatgtgctgg ataccgcctg cgcccgtgtg gccatcaacc
    11101 tgtcgtcgcc gccgaagcaa atctcggcgc tgaccactct gagccaccag caggaggcgg
    11161 aaattcgcca gcttgagcgc gagcttcgca tcggactgcg taccgacaca tcacggatga
    11221 ccgaggtgct ggtgcagtat gatgaaacgc tgacggcgct ggatgaactg gaagcggcct
    11281 ggcaccagca gcagacgctg gtccgggaga ttattgcgct gcgccagcag ttactgggcg
    11341 tggcagagga cgatgcggcg ccgttgccgg acgcagatac cgtggaggat acgcagccag
    11401 agtcagagtc agaacaggat aataccggtg ccgtaccggc tgatgaggcc gacagagaac
    11461 agccggaaga gaccgctgaa acagtttccc cggtacaacg tctggcacat ctcactgccg
    11521 aactggacgc cctgcataac gaccggttgc tggtttcccc gcacgtcgat aaaaaacaga
    11581 ttgcggcggt gattgccgaa tggaccggcg tgccgcttaa ccgcctgtcg cagaatgaaa
    11641 tgtcggtcat caccgacctg ccaaaatggc tcggcgacac catcaaaggc caggacctgg
    11701 cgattgccag cctgcacaaa cacctgctga ccgcacgcgc cgacctgcgc cgtccgggac
    11761 gcccgctggg cgcgttcctg ctggctggcc ccagcggcgt gggtaaaact gaaaccgtcc
    11821 tgcaactggc agagctgctc tacggcggtc gccagtacct gaccaccatc aatatgtccg
    11881 agttccagga gaaacatacc gtctcgcgac tgattggttc gcctccgggc tacgttggct
    11941 acggtgaagg cggcgtactg accgaagcga ttcgccagaa accctactcg gtagtactgc
    12001 tcgatgaagt ggaaaaagcg cacccggatg tgctcaacct gttctaccag gcgttcgata
    12061 agggcgaaat ggcagacggt gaaggccgcc tgattgactg caaaaatatc gtcttcttcc
    12121 tgacgtccaa cctcggctac caggtaatag tcgagcatgc cgatgacccg gaaaccatgc
    12181 aggaagtact gtatccggtg ctggccgact tcttcaaacc tgccctgctg gcgcgtatgg
    12241 aagtggtgcc gtacctgccg ctgtcgaaag agacgctcgc caccatcatt gccgggaaac
    12301 tggcccgcct ggataacgtg ctgcgcagcc gctttggcgc ggaggtgatt atagaaccgg
    12361 aagtgacgga cgaaatcatg agccgcgtca cccgcgcgga aaacggcgcg aggatgctgg
    12421 aatcggtcat cgacggcaat atgctgccgc cgctctccct gctgctgttg cagaaaatgg
    12481 cggcgaatac ggcggttgcc cggattcggt tgtcggcagt ggacggcgca tttacggcag
    12541 acgtggaaga tgctcagaac gacgagtccg tcacaaagga tgaaacggtt ttatgagcag
    12601 gttgcgcgtt ttccggcaga caggatggtt tatcgcgggc ctgatgacgg gcttacccgc
    12661 cactgccgcg cccgcagaag catcgtcaat ggccggtgtg gcggtcgccg tcgccaccac
    12721 gaccccgccg gatgcgacag ccacgttaca ggccatgcag tcctgtcggc gggagtccgc
    12781 ggcgctggag cggcttgact gctatgaccg cctgctggca ccgctgtccc cgtcagggtt
    12841 tgacggggcg ctggtcaaag ccggttttgt gggcgaagcc tggacccgcg ccacggaaca
    12901 ggaaaaacac cgccagggca acaccacaga gctgctggtg acgcaggtgc cgggggagcg
    12961 cccgacggtg gtgataacca ccccggctat cggtcatgtt ccgccacgcc cggtgctgat
    13021 gttcagttgc gtggacaata tcacccggat gcaggtggcg ctgatgcacc ctctggatgt
    13081 ccatgatatc gccgtcactc taaatgccga caaccgggca ttgcgcagcc actggtttgt
    13141 gcgcgagaac ggcaccctgc tggagtccag ccgggggtta tccgggattg atgaaattaa
    13201 gcaactgttc ggcgcaaaaa cgctgacggt ggataccggc gcagacaatg ccgccggaaa
    13261 gctgaccttt aacattgacg ggctggcaag ggcgattgct cccctgcgcg atgcctgcca
    13321 ctgggcggga gaataaaaat atggcaatgg acttacgcga tccgaatgtc tggatatcgc
    13381 acctgctgga aaacctgccg gaagagaaac tggcatcggc gctgaaagat gacaacccga
    13441 actgggagta tatcgacggc gaaatcgtca agctgggatc gctggcccac agtcaactgg
    13501 atatcccgga actccagcgc cgggggctgg tcattctggc ttcggagagt aaggattttc
    13561 gtctgctggc gcatctgctg agaaccctgc aacacgccgg tgatcctcat ctcgcattgc
    13621 gcctgctggc gctgtatgtg gagcattact gggcggtggc tgcgccgcag aatatggcgc
    13681 ataaaaaacg ctttgccagc caggtcatta aacgctttga aacaggtatt gaagtttttt
    13741 cacagaacgc cgccacagcg cagcgcgatg cgctgtcagg ggaactggca aaactggcgc
    13801 aatgctggca gtcacataac gccccggaac tggcacaggc tactgatgat ctttttgccc
    13861 tgtaccagcg tgcgtttaat cgtgcggcac cggctcctgt ccccactccg gcggcctccg
    13921 gtagttcacc acagaccacc gccacgtctg aaagcggcgt gacgcaaccc agtgctccgg
    13981 ctccccaaat cgtcatcgac agtcacgacg acaaagcctg gcgcgacacg ctgttaaaag
    14041 tggcggccat tttatgtgaa cgccagccgg attcaccgca gggctaccgt ctgcgccgcc
    14101 atgcgctttg gcagaacatc accagtacgc cccaggcgga aagcgatggc cgtactccac
    14161 tggctgcggt ctccgccgat atggtggcag attaccatgc tcagctcggt agtgccgata
    14221 tggcgctgtg gcagcaggtg gagaaaagcg tactgctggc tccttactgg ctggatggtc
    14281 actgcctttc tgcacagacg gcgctgcgtc tgggttacaa acaggtggca gacgccatcc
    14341 gcgatgaggt catccgcttc cttgagcgtc tgccccagct taccgggctg ctgtttaatg
    14401 accacacacc gtttatcagt gagcagacga aacaatggct ggcagcttcg cccgacgcca
    14461 aagttgcacc ggttgcgcaa atcggtgagg aatcgaaggc agccagagcc tgttttgctg
    14521 agcagggtct ggaggcggcg ctgcgatatc tggacatgct acccgaaggc gatccccgcg
    14581 atcagtttca ccgccagtac cttgccgcac agttgacgga agaggcgggg ctggtacagc
    14641 ttgcgcagca gcagtaccgg atgttgttca ggatgggatt gcagatgatg gtggcagact
    14701 gggagccgtc cctgcttgag cagctagaac aaaaattcac ggcagaacaa taaaagagaa
    14761 caggagtttt tgtgttcaga ttaccaacac cccgactact cagcggactc aaatcagccc
    14821 tgcgaccggc gatgccccgg tttaaagtct ctgctttctg gctgctgata ctggcgtgga
    14881 tttttctgct ggtgtggatc tggtggaaag gcccgacgtg gacgctgtat gaagaacagt
    14941 ggctcaaacc actggcgaac cgctggctgg caacggcggc gtgggggatt attgccctga
    15001 tgtggcttac cgtccgggtg atgaagcgcc tgcaacagct tgaaaaaatg caaaagcaac
    15061 agcgcgagga agccgttgat ccgctcagcg tggaactgaa cgcccagcag cgttatcttg
    15121 accgctggct gctgcgcctg caacgccatc tcgacaaccg ccgtttcctg tggcagttgc
    15181 cgtggtatat ggtcatcggc ccggcgggca gtggcaaaac gacactgctg cgcgaggggt
    15241 ttccgtccga cattatttat gccccggagg gcgcacgcgg cgcagaacaa cgcctgtacc
    15301 tcacgcccca tgtcggtaaa caggcggtga tctttgatat cgacggcaca ctctgcgctc
    15361 ccgctgatgc ggatatcctg catcgccgtc tgtgggaaca tgccctcggc tggctgaaag
    15421 aaaagcgcgc gcgccagccg ctgaatggga ttattctgac actcgattta cccgatctgc
    15481 ttaccgcaga caaacgccgc cgcgagcatc tgttacaggc gctgcgcagc cgcttgcagg
    15541 atatacgcca gcatcttcac tgccagttac cggtttacgt ggtacttacc cggcttgatt
    15601 tattgcaggg ttttgccgcc ctgtttcagt ccctgaacag acaggatcgc gatgcgattc
    15661 tgggcgtcac gttcacccgc cgtgcccatg aaaatgatga ctggcgaaca gagttgaatg
    15721 ctttctggca gacatgggtg gatcgaatga atctggcgtt gccggatctg atggtcgctc
    15781 agactcacac ccgcgcgtct ttattcagtt tttcccgcca gatgcaggga agccgtgaac
    15841 cgctggtgtc actgcttgag ggtctgcttg atggcgaaaa tatgaacgtg atgctgcgtg
    15901 gtgtctatct cacctcttcg cttcagcgtg gacagatgga tgatatattc acccagtctg
    15961 ccgcccgcca gtaccggctg ggcaataacc cactggcgtc ctggcccctg gtggacaccg
    16021 cgccttattt cacccgcagc ctgttcccgc aggcattact cgcagagcct aatctggcaa
    16081 cagagagccg cgcctggctg atacgttccc gtcgccgcct gacggttttc tctgccacag
    16141 gcggcgtggc agcactgctg ctcatcaccg gctggcatca ctattacaac ggtaactatc
    16201 agtctggcat caccgtgctt aagcaggcca aagcctttat ggacgtgccg cctccgcagg
    16261 gggaagatga ctttggcaac ctgcaactgc cgctcctgaa cccggtacgc gatgccacac
    16321 tggcctatgg cgactggggc gaccgcagcc gtctggccga tatgggactg taccaaggac
    16381 gacgtatcgg gccttatgtg gaacagacct atctgcaact gctggagcaa cgttacctgc
    16441 cctcgctgtt taacgggctg gtcaaagagc tgaacgccgc gccgccggag agtgaagaaa
    16501 aactcgcggt gctgcgcgtg atgcgaatgc tggaggacaa aagcggacgt aacaatcagg
    16561 tggtgaagca gtatatggca aaacgctgga gcgaaaagtt ccacggtcag cgcgatatcc
    16621 aggcacaact gatgtcccat cttgactacg cgctggctca tactgactgg cacgcagagc
    16681 gtcaggcggg cgacggtgac gccatcagcc gctggacgcc atatgacaag cccgtggtat
    16741 cagcacagaa agaactgagc aaactgcctg tctaccagcg ggtttaccag agcctgaaaa
    16801 cgcgggcgct gggcgttctt cctgccgacc tcaatctgcg tgaccaggta gggccaacct
    16861 ttgaccaggt gtttacatct gccgatgaca acaaactggt tgttccacag tttcttaccc
    16921 gttacggcct gcaaagctat tttgtaaaac agcgcgatga actggttgaa ctgacggcga
    16981 tggattcctg ggtacttaac cttacccgca gcgtgaaata cagtgacgcc gaccgcgcgg
    17041 aaatccagcg ccagttgacc gagcagtata tcagcgacta caccgccacc tggcgggccg
    17101 ggatggacaa tctgaatatc cgcaattttg agtccatcgg acaactgacc ggggcgctgg
    17161 agcaggttat cagcggcgac cagcctttgc agcgggcgct aaccgtgctg cgtgacaaca
    17221 cacagccagg cgtcttttct gaaaaactct ctgccaaaga acgggaggaa gccctggcag
    17281 agccggatta ccagttactc acccgcctcg ggcatgaatt cgccccggaa aacagtaccc
    17341 tggcagtaca gaaagacaaa gaaagcacga tgcaggccgt gtatcagcaa ctcaccgagt
    17401 tgcaccgcta cctgctggca atccagaacg cgcctgtacc aggaaaatcg gcgctgaaag
    17461 ccgtgcagtt acggcttgat cagaacagca gcgatccgat attcgccacc cgccagatgg
    17521 caaaaacgct gcctgctccg ctcaaccgct gggttggcag gctggctgac caggcctggc
    17581 atgtggtgat ggtggaggct gttcattata tggaagtgga ctggcgcgac agcgtggtga
    17641 aaccgtttaa cgagcaactg gcaaataact atccgtttaa tccgcgttct gcacaggatg
    17701 cctcactgga tgccttcgaa cgcttcttta aaccggatgg catactggat accttctacc
    17761 agcagaacct gaagctgttt atcgataatg acctgagtct ggaggatggc gataacaacg
    17821 tcattattcg cgaagatatt attgcgcaac tggaaactgc gcagaaaatc cgtgacatct
    17881 tcttcagcaa acagaacggt ctgggaacat cctttgccgt ggaaacggta tcgctttcag
    17941 gcaataaacg ccgcagtgta ctgaaccttg acggtcagtt agtcgattac agccagggcc
    18001 gtaactatac cgcccatctg gtctggccta acaacatgcg cgaaggcaac gaaagtaagc
    18061 tgacgctcat cggcaccagc ggcaacgcgc cgcgcagtat cagcttcagc gggccgtggg
    18121 cgcagttccg cctgttcggg gccggacaac tgaccggagt acaggatggc aactttaccg
    18181 tgcgctttag cgtggacggt ggcgcgatga cctaccgtgt gcataccgac acggaagata
    18241 acccgttcag cggtgggttg ttcagccagt ttggtctgtc agacacactg tactgaggag
    18301 gcagggatga atagtaacgt actgacacaa actatcgtta ccggcagtga cccgcgcggg
    18361 ctgccggaat tcagcgccat ccgcgaggaa ataaacaaag ccagccaccc gtcacagcct
    18421 gagctgaact ggaaactggt ggagtcgctg gcgctggcga tttttaaagc caacggtgtg
    18481 gatttacaca ccgccaccta ctatacgctt gcccggacac ggacccaggg actggcggga
    18541 ttctgcgaag gtgcggaact gctggcggca atggtaagcc acgactggga taagttctgg
    18601 ccgcagggcg gcccggcgcg tactgaaatg ctggactggt ttaactcccg aaccggcaat
    18661 attctgcgtc agcaaatctc ctttgcggaa tccgacctac cactgatcta ccgcacagag
    18721 cgggcattgc agcttatctg cgataagctc cagcaggtgg aactgaagcg cgttccgcgc
    18781 gtggagaatc tgctctattt tatgcagaac acgcgtaaac ggcttgaacc ccagctgaag
    18841 agtaacactg agaacgccgc acagaccacg gtcagaacgc tgatttatgt cccggaaaca
    18901 caggcatctt ccacaccaga agcggtagtg cctcccctgc ccggcctacc tgagatgaaa
    18961 gtggaagtgc gcagtctgac agagaatccc ccacaggcca gtgtgataaa gcaaggcagt
    19021 acggtaagag ggtttatcgc agggatcgcc tgttcagtgg ctgtcgcctc agcattgtgg
    19081 tggtggcagg tctatccggt gcagcagcaa ctgttacagg ttaacgacac cgctcagggc
    19141 gcagcaacgg tgtggatggc ctcacctgaa ctcgaaaact atgagcgcag gctgcaacaa
    19201 cttcttgata cctccccggt acagccgctg gaaaccggga tgcagatgat gcgtgttgcc
    19261 gacagtcgct ggccggaaag cctgcaacag caacaggcct cgacacaatg gaatgaggca
    19321 ctcaaaaccc gcgcacagag tagcccgcag ttgcgtggct ggttgcagac ccgccaggac
    19381 ttacatgctt ttgcagatct agtgatgcag cgcgagaaag agggactaac cctttcctat
    19441 atcaaaaatg tcatctggca ggcggagcgg ggactggggc aggaaacacc cgttgagtct
    19501 ctgttgacgc agtaccatga tgcccgtgcg cagaagcaga atacagatgc gctggaaaaa
    19561 caaattaatg agcgactcga aggcgtgtta agccgctggc tgctgctgaa gaataacgtc
    19621 atgccagagg cggcaaccgg taccacggcc gaaaaataat tatttaatta aaggagatta
    19681 tcatggcaaa tataagttat ttatctttga gtggagagac acaagggcta atttcagcag
    19741 gttgctcaac gcttgattct gtcggaaaca aagcgcagcc agaacataaa gatcagatca
    19801 tggtttatgc gttaatgcat tccatctcaa gaagtcaaaa tgtcaatcat catgaattaa
    19861 ttatcaccaa accggttgat aaatcctcac cattgctggc aaaagcactt agtgacaatg
    19921 aaaaaatggc aatatgtgaa tttatactct accgaaccag taaagcgggt atttaccagc
    19981 cttattataa aataaatctg tcaaaagccc gaatatcaag tattgatttt gtcacaccgc
    20041 atgctgtcct ggaaaaagaa ctggaaccac aggagagaat tgcctttatt tatgaagata
    20101 tttcctggga gcatacgtta gccggtacga atgcaatgag taaatggcag gatcgtgtac
    20161 agtaacttac tgtaataaca taataaatta ctattgcgga gagataaaat ggctggttta
    20221 aggaataaca acaacacaca gaatgcacag tgggctgatt atgtgggtga cattcttcgg
    20281 ggggctcagc cgataaacca gctagtacct cagcaccctt atcttaacga cgtcccactc
    20341 attgatgagt taaggcatca gaacactcat catgttattc tcttgacact tgatgttgca
    20401 aaaaaaatac tcagcccaat aacatcattt gattacattc attttatcac tacacatcct
    20461 tcaggtataa aagatacact cgcctggtta gttaacgcga gtaaattaat gactgagttt
    20521 gatgacaatg ggaaaattat atttaacctt aatgccttaa agtatacaaa agcaagttac
    20581 tttgaaatac ttggagagaa atatataaaa atcacaacat ccagcccctg gcttttagaa
    20641 aagctcggta aatatatttt ctcttcacga gcgcctcaag ttctggaact cgctattggc
    20701 tggcgaggag ctttatctga atcaataaaa ggagttaagt tctgtatctg gttttcagta
    20761 gcctggagaa ccatagaatt tattatgagt tcagagcgag atctggttaa ttttcttggc
    20821 gatttttcaa tggatgtggc caaagcagtt atagccggtg gtgttgcaac cgctattgga
    20881 agcctgactt cttttgcctg tgttagcttt ggctttccag taattcttgt cggaggagca
    20941 attttactga cagggatagt gtgtacagtt gttttaaatg aaatcgatgc tcaatgccat
    21001 ttatcagaaa aattaaaata tgcaattaga gatggactaa aacggcaaca ggaacttgat
    21061 aaatggaaaa gggaaaacat gactccattt atgtatgttc ttaacactcc acccgtgata
    21121 tgaggattaa ccatgcgttt acatgttaaa ttaaaagagt ttctttcgat gttttttatg
    21181 gccatattat ttttccctg
//
LOCUS       DAZGAN020000080        20497 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGAN020000080 DAZGAN020000000
VERSION     DAZGAN020000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.080"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..633)
                     /locus_tag="ACHKYO_RS16495"
                     /old_locus_tag="pgaptmp_003299"
     CDS             complement(<1..633)
                     /locus_tag="ACHKYO_RS16495"
                     /old_locus_tag="pgaptmp_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430091.1"
                     /translation="MNYEKYHSTNRSKGLRTFCYLIYYVQVIFNVMKCFMTRGNFDFI
                     NSNVVFSEADKSLWIDLINTDCIWYDMIINPPDDIRTNKIIKNHLRIMEKYTRESCEK
                     RFIYFLGSRKKVRFSTKKQPRYNPFTKNIVFTLLIGKEERKLRIKYSFCTFTQNGVVK
                     VTPNVRVTEKFLYIEYENGNLCVMSVHDFVNGHYIDLGIHTEVHYVGYTRH"
     gene            complement(792..2027)
                     /locus_tag="ACHKYO_RS16500"
                     /old_locus_tag="pgaptmp_003300"
     CDS             complement(792..2027)
                     /locus_tag="ACHKYO_RS16500"
                     /old_locus_tag="pgaptmp_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4430092.1"
                     /translation="MALSDAWLRSVVGKKRDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELELNRNPRLVKQAEKRKATEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKTPA
                     IVDRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEINILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGDITGEPL
                     KRPIIEPVEELIKYVISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQFAWRRLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYWAWW
                     ERVESIVTCTDLISNGQFA"
     gene            complement(2413..3090)
                     /gene="yjjG"
                     /locus_tag="ACHKYO_RS16505"
                     /old_locus_tag="pgaptmp_003301"
     CDS             complement(2413..3090)
                     /gene="yjjG"
                     /locus_tag="ACHKYO_RS16505"
                     /old_locus_tag="pgaptmp_003301"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HGU4430093.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(3105..3551)
                     /gene="rimI"
                     /locus_tag="ACHKYO_RS16510"
                     /old_locus_tag="pgaptmp_003302"
     CDS             complement(3105..3551)
                     /gene="rimI"
                     /locus_tag="ACHKYO_RS16510"
                     /old_locus_tag="pgaptmp_003302"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGU4430094.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(3520..3933)
                     /gene="holD"
                     /locus_tag="ACHKYO_RS16515"
                     /old_locus_tag="pgaptmp_003303"
     CDS             complement(3520..3933)
                     /gene="holD"
                     /locus_tag="ACHKYO_RS16515"
                     /old_locus_tag="pgaptmp_003303"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HGU4430095.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4036..5067
                     /gene="rsmC"
                     /locus_tag="ACHKYO_RS16520"
                     /old_locus_tag="pgaptmp_003304"
     CDS             4036..5067
                     /gene="rsmC"
                     /locus_tag="ACHKYO_RS16520"
                     /old_locus_tag="pgaptmp_003304"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HGU4430096.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            5335..5421
                     /locus_tag="ACHKYO_RS16525"
                     /old_locus_tag="pgaptmp_003305"
     tRNA            5335..5421
                     /locus_tag="ACHKYO_RS16525"
                     /old_locus_tag="pgaptmp_003305"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5369..5371,aa:Leu,seq:cag)
     gene            complement(5460..5696)
                     /gene="yjjZ"
                     /locus_tag="ACHKYO_RS16530"
                     /old_locus_tag="pgaptmp_003306"
     CDS             complement(5460..5696)
                     /gene="yjjZ"
                     /locus_tag="ACHKYO_RS16530"
                     /old_locus_tag="pgaptmp_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HGU4430097.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            5837..6625
                     /gene="fhuF"
                     /locus_tag="ACHKYO_RS16535"
                     /old_locus_tag="pgaptmp_003307"
     CDS             5837..6625
                     /gene="fhuF"
                     /locus_tag="ACHKYO_RS16535"
                     /old_locus_tag="pgaptmp_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HGU4430098.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(6663..7340)
                     /gene="bglJ"
                     /locus_tag="ACHKYO_RS16540"
                     /old_locus_tag="pgaptmp_003308"
     CDS             complement(6663..7340)
                     /gene="bglJ"
                     /locus_tag="ACHKYO_RS16540"
                     /old_locus_tag="pgaptmp_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HGU4430099.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(7298..8023)
                     /gene="yjjQ"
                     /locus_tag="ACHKYO_RS16545"
                     /old_locus_tag="pgaptmp_003309"
     CDS             complement(7298..8023)
                     /gene="yjjQ"
                     /locus_tag="ACHKYO_RS16545"
                     /old_locus_tag="pgaptmp_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4430100.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            8642..9412
                     /gene="yjjP"
                     /locus_tag="ACHKYO_RS16550"
                     /old_locus_tag="pgaptmp_003310"
     CDS             8642..9412
                     /gene="yjjP"
                     /locus_tag="ACHKYO_RS16550"
                     /old_locus_tag="pgaptmp_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HGU4430101.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            9403..9876
                     /gene="yjjB"
                     /locus_tag="ACHKYO_RS16555"
                     /old_locus_tag="pgaptmp_003311"
     CDS             9403..9876
                     /gene="yjjB"
                     /locus_tag="ACHKYO_RS16555"
                     /old_locus_tag="pgaptmp_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HGU4430102.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            9983..10522
                     /gene="dnaT"
                     /locus_tag="ACHKYO_RS16560"
                     /old_locus_tag="pgaptmp_003312"
     CDS             9983..10522
                     /gene="dnaT"
                     /locus_tag="ACHKYO_RS16560"
                     /old_locus_tag="pgaptmp_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HGU4430103.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPKAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            10525..11262
                     /gene="dnaC"
                     /locus_tag="ACHKYO_RS16565"
                     /old_locus_tag="pgaptmp_003313"
     CDS             10525..11262
                     /gene="dnaC"
                     /locus_tag="ACHKYO_RS16565"
                     /old_locus_tag="pgaptmp_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HGU4430104.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            11311..11805
                     /gene="yjjA"
                     /locus_tag="ACHKYO_RS16570"
                     /old_locus_tag="pgaptmp_003314"
     CDS             11311..11805
                     /gene="yjjA"
                     /locus_tag="ACHKYO_RS16570"
                     /old_locus_tag="pgaptmp_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HGU4430105.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            12059..14350
                     /gene="opgB"
                     /locus_tag="ACHKYO_RS16575"
                     /old_locus_tag="pgaptmp_003315"
     CDS             12059..14350
                     /gene="opgB"
                     /locus_tag="ACHKYO_RS16575"
                     /old_locus_tag="pgaptmp_003315"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HGU4430106.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFCQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(14489..15511)
                     /gene="lgoD"
                     /locus_tag="ACHKYO_RS16580"
                     /old_locus_tag="pgaptmp_003316"
     CDS             complement(14489..15511)
                     /gene="lgoD"
                     /locus_tag="ACHKYO_RS16580"
                     /old_locus_tag="pgaptmp_003316"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HGU4430107.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            15650..16564
                     /gene="lgoR"
                     /locus_tag="ACHKYO_RS16585"
                     /old_locus_tag="pgaptmp_003317"
     CDS             15650..16564
                     /gene="lgoR"
                     /locus_tag="ACHKYO_RS16585"
                     /old_locus_tag="pgaptmp_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HGU4430108.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            16779..18140
                     /gene="lgoT"
                     /locus_tag="ACHKYO_RS16590"
                     /old_locus_tag="pgaptmp_003318"
     CDS             16779..18140
                     /gene="lgoT"
                     /locus_tag="ACHKYO_RS16590"
                     /old_locus_tag="pgaptmp_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4430109.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(18189..19853)
                     /gene="tsr"
                     /locus_tag="ACHKYO_RS16595"
                     /old_locus_tag="pgaptmp_003319"
     CDS             complement(18189..19853)
                     /gene="tsr"
                     /locus_tag="ACHKYO_RS16595"
                     /old_locus_tag="pgaptmp_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HGU4430110.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWAYYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(19972..20418)
                     /gene="hpaR"
                     /locus_tag="ACHKYO_RS16600"
                     /old_locus_tag="pgaptmp_003320"
     CDS             complement(19972..20418)
                     /gene="hpaR"
                     /locus_tag="ACHKYO_RS16600"
                     /old_locus_tag="pgaptmp_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HGU4430111.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         5150 a         5288 c         4813 g         5246 t
ORIGIN      
        1 atgacgtgta tatcctacgt aatgcacttc tgtatgaatt cctaaatcaa tataatgtcc
       61 gtttacgaag tcatgaactg acattacaca taaattgcca ttttcatatt caatgtagag
      121 aaacttctca gttactctaa catttggagt taccttcact accccattct gcgtaaacgt
      181 gcaaaaggaa tattttatcc gaagctttcg ctcttctttg ccaatcaaca gagtgaatac
      241 tatatttttt gtgaatgggt tatacctagg ctgtttcttg gttgaaaacc tcactttttt
      301 tcttgaacct aagaaataaa taaatctctt ttcacagctt tccctcgtat atttttccat
      361 aattctgaga tggttcttta ttatcttatt tgttcttata tcatcaggag gattaattat
      421 catatcatac caaatacaat cggtgttaat gagatcaatc cacaatgact tatcggcttc
      481 tgagaaaacc acgttagagt ttataaaatc aaagtttccg cgagtcataa aacacttcat
      541 cacattaaaa atcacctgca cataataaat taagtagcag aaggttcgca aacccttgct
      601 tctgtttgtg ctatgatatt tttcataatt cattgattgg catccctaca tccttagtcc
      661 cacagtaacc caataaacca tcaaactcct gccgaccccc caaatactga agctaccggt
      721 taacgtagtc tggctaaaaa cccagtacac aaggtatccg cagcggactg gcagatatga
      781 ttacgctctg gctacgcaaa ctgtccgttg gagattaagt cagtacaagt aacgatcgat
      841 tcaaccctct cccaccatgc ccagtaagct ttacgctgtt cttctagata atcgctctta
      901 tcataaactt gccatacacc tggcagttta tgaccgagca ttatttcagc aatatgagga
      961 gcagtaagat cagaaaagtt tgttcgtgct gttcgtctca aatcatgaag agaccaatga
     1021 ggaaattgat accccaaacg tcgccatgcg aactgcatta aattgtaagg cagcgactgc
     1081 aatgatgtcc gaccaactgg ctccctgctt ccttccttgg taaaaagcat atcggaaccg
     1141 ttgttcatag agataacgta ttttataagc tcttcaaccg gttcaataat gggccgcttt
     1201 agcggttcgc ctgttatgtc cccagtctta tgtcgttctg gtggtacagt ccatacctta
     1261 tttatgaaat caaaatcatc caccctagca gtaattagct ctgaactacg gcaaccaaaa
     1321 tgcagcaata gtttaatgaa ggctcggtat ttaggaacca ttcgagaacc atcgatcgca
     1381 gcataaagga tgttaatttc atcatgtgtc agaaaccgtt ttttctgacc tttacggata
     1441 tccatatctt tacccgtgat atccgacagt gggcgagttt caatgagctt tctcttatac
     1501 gcccagacat gagcctgctt tgcgttaatt agcaatcggt ctactattgc tggagtcttg
     1561 gtgctaagag gctccaggac ttctaaccaa tcatgcaatg tagctgcatc gtgagggata
     1621 ttcccgattt tagagaacag gtgcagctca aacgagcgga gtatctgttc agaacctttt
     1681 ttatttttta cacaatatgc ttcataccag gcacggatca cagactctac cgtcatggct
     1741 tcagtagctt ttcgtttttc agcctgcttg actaatcgtg gattgcggtt taactcgagt
     1801 tcaccacgga gacggataac ttcttctctg gcctctttta atccagttgc cgggtaagtt
     1861 ccgatatcaa ggcgctcacc tttccctgcc cattgataac gatattggaa cactacgcga
     1921 cctttcggtg atactctgac agacagacca tcacgatcgg atttaaccaa aaccttatca
     1981 cgtttctttc caacgactga acgcaaccac gcatcagaca gcgccattac tcaccttgta
     2041 caaatcgcaa aacacctctg ttgctttgta cattatgtac aagcatatgt acagattttt
     2101 gatgaaagca acctgatcga tttaaagtta catgaaagaa ttttcaatta aagaagccac
     2161 ataactaact gtatttaaat gacttataaa acaaaacatc aattaactga aaggactttg
     2221 aaaaagtaaa agagctttaa tttaggctta attattgggg tgagaaataa tggcaaaagt
     2281 gcggcgcttc gccacctctt gcaaataagg agacaacgtc ataattcttt cttcttgagt
     2341 aagcggcatc gcgccgcgct tgttgaaaac gaaaaattgc ggctatttta cccatcaacg
     2401 ggggggaggc aatcagtgtt tacacaggag ctgctccagt tcatgcaacg aagaaacggt
     2461 ccaggtgggc gcgatgcctt ctggttgctc gcgatggtgt gcattcagcc agcaggtcgc
     2521 aagcccggcg ttgatgccac cgagaatatc ggactcggca gtgtcgccaa ccatcagcac
     2581 gcgtgaacgg tcaggattgc ccgcctgttc cagcgcataa tcgaaaattt tcttattcgg
     2641 tttagcaacg ccaacttctt cggaaatcac cagcaaatcg aagtaatcac gcaggcccgt
     2701 ccgttccaga cgcacttgct gcaaggcgct aaaaccgttg gtgatgatgc cgattttagc
     2761 gttgccacga atggcgttaa gcagagaaac cgcgcccggc aacggcgtac agatttccgc
     2821 catcgcatta ataaaggcat cgttaagcaa gcctgcttca actttcagcc gttcggccca
     2881 gctctcaaac cgcccgtgct gaagctgtaa tgaagtgatc gcgccgtttt gataatccac
     2941 ccacagtggc ttgttaacgg cctgatagtc ctgaaaatct tcagcggtaa aggtgacgct
     3001 gtaatcaaga aacatccgct gcaggccggt gaatgagtca aaggtaaaca gcgtttcatc
     3061 ggcatcaaag aaaatccagt cccacttcat tattccacct tgtcttacat actgattggc
     3121 aacgccatga tgatggcgtc ttcgcgaccg tccgtggtgg ggtagtaatt gcggcgaatc
     3181 gtcgcctcgt taaagcctaa actttcgtac agggcaatgg cggcagcgtt tgaagcacgg
     3241 acttccagcc atagtgtcgc cacgccgcgt ttttccagtt cgtcgatcag atgttccagc
     3301 agcacccttc ccaatccctg acgctgatag tcaggatcga ccgcaatatt gaacaatgta
     3361 gcttcatcca gcaccacttg cgtaatcgca aacgccgcca ttttgccgtt ttgcgttaac
     3421 tgaaagttga gataacgctc gccctggttg ctggcaaagg ttttttcact ccacggaaag
     3481 gcgtgggcgc gttgttcaat gtggtaagcc gctggtaaat cagtcgtttc gagggaagaa
     3541 atcgtgttca tatgtgcaaa tctgttgcca taacgcggcg cgtgccgttg ggtttgcccg
     3601 taattcggtg agcgccggtg atgccacctg agcgccttcc agtgatagcg gttcgtcagt
     3661 acccaaccgc cagctgttgc agcgactgcc ttgcggcagc atcgcgattt tttctggcgt
     3721 cagttgcagc acttgatcgg ggctgacggt taatgcgcgc agaacatcgc tcactaaagg
     3781 atcggtcagg gcgggaagat cgtttgccac catcaccaga cggacgtgtg ccgggatcgc
     3841 aatggcaatc tccccctgca acgcgccagg gcgacgcagc gaccactggg tgatgcccag
     3901 ttgctgtaac tgccagtctc gtcgggatgt catagcgaat cgctcctgtt gtcaggggcg
     3961 caaatatagc aaattcgtcg ataccgcgcc aacatatggc tataatcgcc gccagtatca
     4021 attgaggagc attccatgtc tgcatttacc ccggcaagtg aagtcttgct gcgtcacagt
     4081 gatgatttcg aacaaagccg tattctgttt gccggagact tacaggatga cctgcccgcg
     4141 cgtttagata ccgcggccag ccgtgctcat acccagcaat tccaccactg gcaggtatta
     4201 agccgccaga tgggggataa cgcccgtttc agtctggtcg ccacggtgga tgacgtcgca
     4261 gattgcgata cgctgattta ctactggccg aagaacaaac cggaagccca gttccagttg
     4321 atgaatttac tttctctgct gccagtgggg acagatattt ttgtcgttgg cgagaaccgc
     4381 agcggcgtgc gcagcgccga gcagatgctg gcagattatg cgccgttgaa taaagtcgac
     4441 agcgctcgtc gctgtggcct ctattttggt cgtctggaaa aacagccggt atttgatgcc
     4501 gataaattct ggggcgaata cagcgtcgat ggcctgacgg tcaaaacgct gcctggcgtg
     4561 tttagccgcg acggtctgga tgtcggtagc cagttgctgc tctcgacgtt aaccccgcac
     4621 acgaaaggta aagtgctgga tgtcggctgt ggcgcgggtg tgctttcagt tgcctttgcg
     4681 cgccattcgc cgaaaattcg tctcaccttg tgcgatgtct ctgcgccagc ggtagaagcc
     4741 agccgcgcaa cacttgcggc caacggtgtt gaaggtgaag tctttgccag caacgtcttt
     4801 tccgaggtga aaggtcgttt tgatatgatc atctccaacc cgccgttcca cgatgggatg
     4861 caaaccagcc tggatgcggc gcaaacgctg attcgcggcg cggtgcgtca tcttaatagc
     4921 ggcggcgagc tgcgaattgt agcgaacgcc ttcctgcctt acccggacgt gctggatgag
     4981 acatttggct tccatgaagt gattgcgcaa accgggcgtt tcaaggtgta tcgcgccatt
     5041 atgacccgcc aggcgaagaa aggttaatta tctcgccgaa taccgtgtcg gatgcggcgt
     5101 gaacgcctta tccgacctac aaaacctgca cgttagccct tcgtaggcca gataagacgc
     5161 gccagcgtcg catctggcat ctgcccccgc atgcctacca aacctgcacg ctagtttcct
     5221 gatggacatt tttccagcaa ttacacctcc ggcgataatt aactattgac gaaaagctga
     5281 aaaccactag aatgcgcctc cgtggtagca attcttttta agaattgatg gtatgcgaag
     5341 gtggcggaat tggtagacgc gctagcttca ggtgttagtg tccttacgga cgtgggggtt
     5401 caagtccccc ccctcgcacc aattatcttt acttcctttc ttgtttcttc cttgattttt
     5461 catccctgat tcaaattcat tatcatcagc atgatgccag caataagcgc caggcacgat
     5521 ggtagcaaaa cgtaatgccg taactgtttg tgatacagca tcaccggcgt caatactaat
     5581 cccatcagaa tgacaatccg ccactgatcc ggcgataaat ccgcatacat cagccctgca
     5641 atcagcaatc ctggcagcaa cactccccag ccactgccca gcatacgttg caacatggtt
     5701 tcatctcttt cattgataat gataaccaat atcatatgat aatttttatc atttgcaagc
     5761 cagagaaatc ccttgctatc gggtaaacct atcgctagga ttagcaatca ttatcattta
     5821 gattactatc ccgattatgg cctatcgttc cgcaccactc tatgaagata tcatctggcg
     5881 aacgcatctc cagccgcagg atgctgggct tgcacaagcc gtacgtgcga cgatcgccaa
     5941 acatcgtgaa catttgctcg agtttatccg cctggatgaa cccgctccgc ttaacgccat
     6001 gacgctggcg caatggtcat caccgaatgc gctaagttct ctactcgcgg tctattccga
     6061 tcatatctat cgcaaccaac caacgatgat ccgcgagaac aaaccgctga tctcactatg
     6121 ggcacaatgg tatatcggcc tgatggtgcc accattaatg ctggcgctac tgacgcagga
     6181 aaaggcatta gatgtgtcgc cggaacattt ccatgctgag tttcacgaaa ccggacgcgt
     6241 cgcctgtttc tgggtcgatg tgtgtgaaga taaaaacgca acaccacatt cgccgcagca
     6301 gcgaatggaa acgttaatca gccaggcgct ggttccggtt gtgcaggcac tagaagcgac
     6361 cggagagatc aacggcaaac ttatctggag taataccggt tatttgatca actggtatct
     6421 cactgagatg aagcaactgc tcggcgaggc gacggttgaa tcgctgcgcc atgccctctt
     6481 ttttgagaaa acgcttacta ccggtgaaga taatccactc tggcgtaccg tggtgctacg
     6541 cgacggcctg ctggtgcgcc ggacctgctg ccagcgttat cgcttaccgg atgtgcagca
     6601 atgtggcgat tgtacgctga aatgaaagca ctgccgggga agtaaacccg gcatcatgcg
     6661 gattaatagg gatgcaacac attacttgct tcgcaatgcc gcatacataa cagaatatct
     6721 gcggcctcca acaaccctgc atcagaactg acgcccagtt tcgacatcac attaaattta
     6781 tgtgcacgga tcgttttgat attgcgttta agctgctcgg caatttgtgt cattgagtag
     6841 ccacgcgaca taaagcgcaa tatttctctc tccgtcgggc ttagcgtccg gctttggtta
     6901 atgtaccact gattgttcag tcggtcggtc gcctgacgta caccatttaa tgacaaaaag
     6961 agttcctgat gaaaaatctc cagcgttgac gctttactta atacaccgtc cagcggtgat
     7021 ggcgacaatg aaccaatcag ccgagcttca atatcatcat ccgcaataac taaacgccgg
     7081 gtacgcggaa acttaatcgc cagttcagtc aggcaagata atccctctct gcgctcactt
     7141 ctcatggcag aaaaagaaaa aatgaccgcc gcaaatgaga tccgcgacat ggcatcctga
     7201 aatgactcct gactggtata ggtatggagc ttataagggt tacccgcaaa ctttctgaat
     7261 aaactctcaa taccgatact gctcatgacg catttttcta tgagtgcgac atttctcttc
     7321 ttaattcggc tgtgttccat tctgcggcat ctccataaac agaaggcaag ttaagttctc
     7381 ttgcaccctg cgcgctattt atccatgcat acatttcagc gttgctgtga attgccagtc
     7441 gacgcatcgc actgtttttt tgggcgctga tagttttatt acttttctta agcaacgaag
     7501 ctatctggtt gatgccccat cctttaccta atagccgcag taccttgcgc tctgaaagag
     7561 tgagaatgac ggagcgatag ctaaaatcat gagtttcatt cattgccgga gaggtcagca
     7621 tggcgctgat acgctctgcg tgcgtattgc cggaacgtac ggtcttgacc agattctcaa
     7681 tgggttcaac atcagacaat aacgtcgccg tagggcacat gagcaattct actgcctggg
     7741 aataccagga gcgtgacacc atgaaaaccc agtgaatttc ccgatattgt gagattaaag
     7801 aataataatg ttcacaaaca gaacgaggat cttcactttc accggctaaa tcagcaatga
     7861 ctaatccgga acgacgtaat tgtaataagg taaggtcctc cgcagaggcg ctggatatta
     7921 tttcatattc agggaagtga gtcctcatga cctcttttaa ccctgcttgc ataacaggta
     7981 ttttactgat aacaattcca tttttgcagc atcctggcaa catatttcct ccacatccac
     8041 tgtctaaaaa tcatgatggt ttattcagga tattcattcc ttaaatgttt ccagcaaatt
     8101 aatagattaa gcgaataaat tatccttagc attcactttc atatcagcat actcctgctg
     8161 cacaacaact ccactgcatg tgtcagaagg ttagcatcaa atgataacct ggtgaaatag
     8221 cctggagaca tttaaaatat ctacattaag ttgttaggaa tacttaatga aaaacagaaa
     8281 tcacattcat ttaatttata attaaatgat atcccttccc gcagaatgaa aaatcacaca
     8341 accaatattt attcaatgaa atctgatggt tttaacttgt taacattaaa aaacgtgccc
     8401 ggttatctat tatcctgatt cgtaaaggcc aaacaataaa ttcattatag tgataatatt
     8461 catgtgatag tttgtttgcg gcgagagata attcgctttt tatcaccgag cttgtcccga
     8521 taaccaggat taatcagcgc aattttgatc ttaacctacc gcaaaacaac cgtgcttcat
     8581 gatacaaatg gattctcatt atctgaacaa tacgcagcac gtctatgaca aagggcgagt
     8641 tatgcaaact gagcaacagc gagccgtaac acggctttgt atccagtgtg gattatttct
     8701 tttgcaacat ggtgcggaaa gcgcgttggt tgatgagctt tcctcacgac tgggtcgggc
     8761 gttggggatg gacagcgtcg aaagttctat ctcttcgaac gccatagtgc tgacaaccat
     8821 taaagatggg caatgcctga catcgacacg taaaaatcac gatcgcggca ttaatatgca
     8881 tgtggtgact gaagtccagc acattgtgat tctggcggag caccatctgc tggattacaa
     8941 aggcgtagag aaacgattta gccaaattca gccattacgt tacccaagat ggctggtagc
     9001 cttaatggtt ggcctttctt gtgcctgttt ctgtaaactc aataaaggtg gctgggatgg
     9061 tgccgtcatc accttctttg ccagtacggc cgcgatgtat atccgccagc tgctggcaca
     9121 acgtcatctt catccacaaa tcaacttttg ccttaccgct ttcgccgcca ccaccatttc
     9181 cggattgctt ttgcaacttc ccactttcag caatacaccc actatcgcga tggccgccag
     9241 cgttctgctg ctggtgccgg gatttccgtt gattaatgcc gtcgccgata tgtttaaagg
     9301 ccacatcaat accggactgg cacgctgggc gatcgccagt ctgctgacac tggctacctg
     9361 cgtcggcgta gtgatggcac tgacgatttg ggggctacgc ggatgggtgt gatcgaattt
     9421 ctgttagcgt tggcgcagga tatgatcctc gccgccattc ctgcggtcgg ctttgcgatg
     9481 gtgttcaacg ttcccgttcg ggcgttacgc tggtgtgcgc tgcttggcgc gattggtcat
     9541 ggttcacgaa tgatcttaat gaccagcggg ctgaatattg agtggtcaac ttttatggct
     9601 tctatgctgg tcggcaccat tggtattcaa tggtcgcgct ggtatctggc gcatccgaaa
     9661 gtgtttaccg tggcggccgt tatccctatg ttcccgggca tatcggctta taccgcaatg
     9721 atttcggcgg taaaaatcag ccagttaggt tacagcgaac cgttgatgat taccctgtta
     9781 accaactttc ttacagcttc atcgattgtt ggtgcgttat ccatcggtct ttccattcct
     9841 ggattatggt tgtaccgcaa gcgccctcgc gtataaaatt gcctcgctta agcacacgga
     9901 tgagagacag cctcctctcc tccgtgtgtt actataaaag ttatctccct tctcgttcat
     9961 cgttccatat ttgagaaaca gtatgtcttc cagagttttg accccggacg tcgttggtat
    10021 tgacgccctg gtacacgatc accaaaccgt tctggcaaaa gctgaaggcg gtgtggttgc
    10081 cgtatttgct aacaatgccc cggcgtttta tgccgtcacg cctgcacgcc tggctgaact
    10141 gctggcgctg gaagaaaagc tggcgcgtcc gggaagcgat gtcgctctgg acgatcaact
    10201 ctatcaggaa ccgaaagccg ctcccgttgc tgtccccatg gggaaattcg ccatgtatcc
    10261 ggactggcaa cccgatgccg actttatccg cctggcggcg ctatggggcg tggcgctaag
    10321 agagccggtg accaccgaag aactggcctc attcattgcc tactggcagg cggaaggtaa
    10381 agtctttcac catgtgcagt ggcaacaaaa actggcgcgc agcctgcaaa tcggtcgtgc
    10441 cagcaacggc ggactgccga aacgagatgt gaatacggtc agcgaacctg acagccaaat
    10501 tccaccagga ttcagagggt aacgatgaaa aacgttggcg acctgatgca acgcctgcaa
    10561 aaaatgatgc ctgcccatat caaacctgca ttcaaaacgg gtgaagagct actggcctgg
    10621 cagaaagaac aaggagcgat ccgctccgcc gctctcgaac gtgaaaatcg ggcgatgaaa
    10681 atgcagcgca cctttaaccg ctccggtatt cggccactgc atcagaactg ctcctttgag
    10741 aactatcgcg ttgagtgtga agggcagatg aatgcgttaa gcaaagcgcg ccagtacgtc
    10801 gaagagtttg acggcaacat cgccagcttc atcttttctg gtaagccagg aaccggcaaa
    10861 aaccatctgg cggcggcaat ctgcaacgag ctgctgctac gcggtaaatc cgtattgatc
    10921 atcaccgtgg ccgatattat gtcggcgatg aaagatacct tcaggaatag cggtaccagt
    10981 gaagaacaac tgcttaacga tctgagcaac gttgatctgc tggtgatcga tgagatcggc
    11041 gtgcagaccg aatcgaaata cgaaaaagtg atcatcaacc agatcgtcga tcgccgctct
    11101 tcttccaaac gcccaaccgg gatgctgacc aacagtaata tggaagagat gaccaagttg
    11161 ctgggcgaac gcgtgatgga ccgtatgcgc ctgggtaaca gtttgtgggt gatcttcaac
    11221 tgggatagct accgtagccg tgtaacaggt aaagagtatt aatccccgtt tctggcggta
    11281 tactgcgaca caattccacc ttgagtaatg atgatgaaaa ctgttaaaca tcttctgtgc
    11341 tgtgccattg ccgccagcgc attaatttcc accggggtgc atgctgcgtc ctggaaagat
    11401 gcgctctcca gcgccgccag cgaacttgga aaccaaaaca gcacgacaca ggaaggcggt
    11461 tggtcgctcg cgtcattaac taacttgctt agcagcggga accaggcctt aagcgcagat
    11521 aacatgaaca acgccgcagg cattctgcaa tactgcgcga agcaaaagct ggcgtcggta
    11581 accgatgccg aaaacatcaa gaaccaggtg ctggaaaagc tgggcctgaa cagtgaagag
    11641 caaaaagaag acaccaacta tctggatggc attcagggtt tgctgaaaac caaagatggt
    11701 cagcaactca atctggataa catcggaacg actccgctgg cagaaaaggt gaaaaccaaa
    11761 gcctgcgatc tggtgttaaa gcaggggctg aacttcattt cctgaaccgc gaaacgccgc
    11821 gttttagctg ttacccggct gacgcggctc tctcctgccc ttttccctgc tcctggacgg
    11881 ttttccccct gttacacggg aagcgaccgc gatcaaaaaa gtctgtttat aaactaattc
    11941 ctaacaacat cgcatttttt tgacactaga attgcagcaa tatggcgacg ccattacatt
    12001 gtgcttctca aaaagcttaa acgttggcta aacggcccgg tttagtgagg atcgcccgtt
    12061 gtcagaacta ctctctttcg ccctttttct cgcctctgtg ctgatttacg catggaaagc
    12121 gggacgtaac acctggtggt ttgcagccac attaacggtg ctggggctat ttgtcgtttt
    12181 aaatatcacc ctgtttgcca gcgactattt tactggcgat ggtattaacg acgcggttct
    12241 ctatacctta accaacagcc tgaccggtgc tggcgtcagc aaatacatcc tgccgggtat
    12301 cggcattgtg ctggggctga cagcggtgtt cggtgcgctg ggctggatcc tgcgccgtcg
    12361 tcgccatcat ccgcaccatt ttggttacag cctgctggcg ctcttactgg cgctgggttc
    12421 ggtggacgcc agcccagcat tttgtcagat aacggaactg gtgaaatccc agtcacgcga
    12481 tggcgacccg gactttgcgg cttattataa agagccgtcg aaaactatcc ctgacccgaa
    12541 actcaacctg gtttatatct acggcgaaag tctcgagcgg acctattttg ataacgaagc
    12601 tttcccggat ctcacgcctg aactgggcgc gttgaaaaat gaaggcctgg atttcagcca
    12661 cacgcagcag ctgccaggaa cggattacac gattgcgggc atggtggctt ctcagtgcgg
    12721 cataccgctg tttgccccct ttgaaggcaa cgcctccgcc tctgtctcca gcttcttccc
    12781 gcagaacatc tgtctgggcg atatcctgaa aaactcgggt tatcagaact atttcgtgca
    12841 gggcgcgaat ctgcgttttg ccggtaaaga tgtgttcctg aagtcgcatg gcttcgacca
    12901 cttatacggc tcagaagagc tgaaaagcgt ggtggccgac ccgcactatc gcaacgactg
    12961 gggattctac gacgataccg ttctcgatga agcgtggaaa aagtttgaag agctttcccg
    13021 ctcaggtcag cgattctcac tgtttaccct gacagtcgat acccatcacc cggatggttt
    13081 tatctcgcgt acctgtaacc gcaaaaaata tgattttgac ggtaagccga accagtcatt
    13141 cagcgcggta agttgcagcc aggagaatat cgcggcgttt atcaacaaaa tcaaagcgtc
    13201 accgtggttt aaagataccg tcatcgtcgt ctcttctgac catttagcga tgaacaacac
    13261 ggcgtggaaa taccttaata agcaggatcg caataacctg ttttttgtca ttcgtggcga
    13321 caagccgcag caagagacgc tggcagtgaa gcgtaacacg atggataacg gcgcgacggt
    13381 gctggacatt ctcggtggcg ataactatct cggacttggt cgtagcagtt tatccgggca
    13441 gtcaatgtcg gaaatcttcc tcaatatcaa agagaaaaca ttggcgtgga agccggatat
    13501 catccgcctg tggaaattcc ctaaagagat gaaagagttc accatcgacc agcagaaaaa
    13561 catgattgcc ttctcgggta gccatttccg tttgccgctg ctgttgcggg tttcagacaa
    13621 gcgcgtagaa ccgctgccgg aaagtgaata ctcagcaccg ctgcgtttcc agctggccga
    13681 tttcgctcca cgcgacaatt tcgtctgggt tgaccgttgc tacaagatgg cacaactctg
    13741 ggctccggaa ctggcactct ccaccgactg gtgtgtctcg caggggcagc ttggcggtca
    13801 gcaaattgtc cagcatgttg ataaaacaat gtggaagggc aaaacggcat ttaaagatac
    13861 ggtcatcgac atggcgcgtt acaaaagcaa tgtcgatacg ctgaaaattg ttgataacga
    13921 tattcgctac aaagccgaca gtttcatctt caacgtcgcc ggtgcgccag aagaggtgaa
    13981 acagtttagc gggatttccc gtccggagtc gtggggccgc tggtccaacg ctcagctggg
    14041 cgatgaagta aaaatcgagt acaagcatcc gctgccgaag aaatttgacc tggtgattac
    14101 cgccaaagca tacggcaata acgccagccg tcctattccg gtacgcgtag gcaatgaaga
    14161 acaaactctt gtgctgggca atgaagtaac caccaccaca ctgcatttcg ataacccaac
    14221 cgatgccgac acactggtaa ttgtgccgcc ggaacctgtc tcaaccaacg aagggaatat
    14281 cctcggacac tcgccgcgta agctcgggat cggcatggtg gaaattaaag ttgtggaacg
    14341 tgaagggtaa tttctgactg atgtgtaggt cgcataaggt gcgacctaca caccttctcc
    14401 ggcctacaaa acatcattga gaaacagaaa aaaacagcgc ccaacacttc cccggacgct
    14461 gcatggcaac tttaataatc tcgccttatc agaaagtaat tacaccctta atcaactcac
    14521 gattgttaat cacatcgcgc tcgtaggttt ctgccagcgt ggcgaacgga tagcgatggg
    14581 ttaacatcat gtcggcagtg attttccctt ctgccatcag tcgaccgact ttagcgaaat
    14641 cttccggcgt ggcgttgcgg ctgcccatca tcgtcgtttc ttttttatgg aattccggat
    14701 cggagaactg caactcacct ttaaacaggc cgacaaatac caccgtgccg ccgtgacgaa
    14761 tcaaattcac ggtgttattc atcgcatgtt gattacctgt cgcgtcgatc actttctgcg
    14821 ccagcgaacc accaaactgc gcccgtagct gcgcgtcaaa atcttcggct gacgggtcca
    14881 gtaaaggtaa ttccagacgc gttgccacat gttcacgacg cgccggactg gtatccgcca
    14941 ccaccacctg tgcgccatcg gctttagcga ttgccgccgc gcccagaccg attggccccg
    15001 ccccgaccac cagcacctgc tcgccgggag caatggctgc gcgacgcacc gcatgagcgc
    15061 taatagcgaa aggttcaatc aatgctgccg cctgcgggtc aataccgtct gcgggcaaaa
    15121 tgttcgccac cggcacgctc aaatactcac taaaaccgcc atcctgatgc acgccaatga
    15181 ctgaaatttt ttcacagcaa ttggtacgcc cgcttttaca cgccgggcat tgctgacagg
    15241 caacataagg gatcacagca acttgctgac catttttaag atcagcaata tttttaccca
    15301 gcccaacaat ctccccacat atttcatggc ctaaaacacg tggataacta aaaaatggtt
    15361 gatttccacc ccaggcatga atatcggtac cgcaaatccc gacagactta atttttatta
    15421 atgcttcatt gtcacccgga ataggtatct cgcgttgttt ccagactaat tctttcggct
    15481 gctggcaaat taaaacattc atcgtagaca tgattcggct ctccattttt tgttgcctta
    15541 gatatcaatg aaaatgaaaa atggcagcgg tacggaaact ctgttttgtg aaaacacacg
    15601 cataaaaatg ttttaaatcg ggttttaatg gactcacaag gaggaaaata tgagtcgttc
    15661 acaaaattta cgccacaatg tgattaacca ggtcattgat gatatggcgc ggggtcatat
    15721 cccttcccca cttccatcgc aaagtgcgct ggcggaaatg tacaacatca gccgcaccac
    15781 agtgcgtcat attctcagcc atttacgcga atgcggcgtc ttgacgcagg ttggaaacga
    15841 ctatgttatt gcgcgtaaac ctgaccacga tgacggtttc gcctgtacta ccgcttcaat
    15901 gagtgaacaa aacaaagtgt ttgagcaggc ttttttcacg atgataaacc agcgccagtt
    15961 acgcccaggg gaaacttttt ctgaactgca actggctcgg gccgcgggtg tcagcccggt
    16021 ggttgtaaga gaatatcttt taaaattcgg ccgttataat cttattcaaa gcgaaaagcg
    16081 cggccagtgg agcatgaagc aattcgatca atcctacgcc gagcagctgt ttgagctacg
    16141 cgaaatgctc gaaacacatt cacttcagca ctttctcaac ctgcctgatc atgatccccg
    16201 ctggctgcag gcgaaaacca tgctcgaacg tcatcggcta ctgcgtgaca acatcggcaa
    16261 tagctttcgc atgttctcgc aacttgacag agattttcac tccttactgc tttcagctgc
    16321 cgacaatatc ttttttgatc aatcgcttga gatcatctca gtcatctttc attttcacta
    16381 tcaatgggac gaaagcgatc ttaaacagcg caatatcatt gccgtcgatg agcatatgac
    16441 cattctcagc gctctgattt gccgcagtga ccttgatgcc acccttgcgc tccgtaacca
    16501 tttgaattca gctaaacaat caatgattcg atccattaat gagaacacgc gttatgctca
    16561 ttaagtacgg attaaaaaca gcaaactatt agcgggtgca cggttttatc aaaagataaa
    16621 taccgggaaa ttcccgctta cctatgctca caatcagtcg actaaaaatg tttttgtgag
    16681 cggtagtaaa gtcctaaaac tttaacctgc cgttaatgtc aaccttgcgt aattcacaat
    16741 tacgtaaaca attaaaatta taaatacagg agtccggcgt ggaaaaagaa aatatcaccc
    16801 tcgatccgcg ttcttcattt actccatctt cgtcggcaga tattcccgtg ccaccagatg
    16861 gattagttca acgcagtacc cgaattaaac gcattcaaac caccgccatg ttgttattat
    16921 tttttgcggc ggtaatcaat tatctcgacc gcagttcgct gtcggtagca aatttaacga
    16981 ttcgtgaaga attgggatta agtgccaccg aaatcggcgc tttgctctcc gtgttttcac
    17041 tcgcttacgg gattgcgcaa cttccttgcg gcccactatt ggatcgtaaa ggcccacgcc
    17101 tgatgctggg actggggatg ttcttctggt cactgttcca ggcaatgtct ggcatggtgc
    17161 acaactttac gcagttcgtg ttggtgcgta tcggtatggg gattggtgaa gcgccgatga
    17221 acccatgcgg tgtaaaagtc attaacgact ggttcaacat caaagagcgc ggacgcccga
    17281 tgggcttctt caacgcagct tctaccattg gcgttgccgt aagcccaccg attctggcgg
    17341 cgatgatgct ggtgatgggc tggcgcggga tgtttattac cattggtgta ctggggattt
    17401 ttctcgccat cggctggtat atgctctatc gcaaccgcga gcacgtagaa ctgactgccg
    17461 ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg ccgagatccg ctcagttttg
    17521 ccgaatggcg cagcctgttc cgtaaccgta caatgtgggg aatgatgctc ggattcagtg
    17581 gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc tggttacctg caaacagcct
    17641 ataacctgga tttaaaaagc acagggttga tggcggctat ccctttcctg tttggggctg
    17701 ccgggatgct ggtcaacggt tacgttactg actggctggt caaaggggga atggctccga
    17761 ttaaaagccg taagatctgc attattgccg ggatgttctg ttctgccgcc tttacgctga
    17821 tagtaccaca agcgacaaca tccatgacgg cggttctgct gattggcatg gcactgttct
    17881 gtattcactt tgccggaaca tcctgctggg gcttgatcca cgtcgcagtt gcttctcgca
    17941 tgactgcgtc ggtgggcagt atccagaact ttgccagctt catctgcgcc tcttttgcgc
    18001 cgatcattac tggttttatt gttgatacca cccactcatt ccgtctggca ctaatcatct
    18061 gcggttgcgt caccgcagcg ggggcactgg cgtacatctt cctggttcgt cagccgatca
    18121 acgacccacg taaagattaa tctccctatg cccgataaca ttttgcttat cgggcatttt
    18181 tatggcgatt aaaacgtttc ccagttctcc ccgctatctg ccactgccat tttacgcggc
    18241 gtagctggcg tcacggtttt taccacagcc gatgtttcac gctgctgttg ctgctgttgc
    18301 tgaatccgga acactgccac agcttcggtc aggcgactgg cctgctcttc cagcgcggcg
    18361 gcggcggcgg cagattcttc caccagcgcg gcgttctgtt gagttacccg gtccatctca
    18421 gcaaccgcta agccaacctg atcgatacca cggctctgct catcagaagc agaagctatt
    18481 tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa tctccgccat tgtttccccg
    18541 gcgctttcga ccagcgtaga gccaacatcc actttcccca ccgagtcttc aatcaggctt
    18601 ttaatttcac gagccgcctg ggcgctacgc tgggccagat tacgcacttc tcccgcaacc
    18661 accgcaaaac cgcgcccttg ctcacccgca cgcgcagcct caaccgccgc gttcaaagcc
    18721 agaatattgg tctggaaggc aatgccgtca attacgctga taatatcggc gattttctgc
    18781 gaactggtgg agatatcgcg catagtctgc accacgttat ccaccacttt gccgccgcgt
    18841 tgcgccgttt cagaagcact taacgccaga tggctggcct ggcgcgcatt ttcggcgttc
    18901 tgcttcaccg ttgcggtcag ttgctccatg ctggctgccg tctcttccag cgaagcggcc
    18961 tgttgctcgg tgcgcgaaga gagatcgtta ttgccggtgg cgatttcgct ggcaccgcta
    19021 tagatggcat tggccccgtt gcgcacatca ccgacggtac gcatcagctc tccctgcata
    19081 tggcgcaaac tctctgccag ttgccccatc tcattagagc catccacctc aatcggtttc
    19141 accagatcgc cgcctgcaat atgacgaatg ctgtcaatca ggcgattcat tggcgctacc
    19201 agcgaggctt taataccgaa ccagacggcg aagatgaccg ccagtacgac gatcatcacg
    19261 cccaccagaa tccacatagc ctggctgtag gaggcattgt tatcgctgac ggcgatatca
    19321 tagagccgat cgttttgctc catgtaagcc acatactgct tctcgaaacc gtcctgatat
    19381 ccctgggtcg gctgatcaaa gaactcgttg attttgcctg cacctaatag ttggatcagc
    19441 tccgccagcg cattgtgata aatatcgtaa ttacgtttga tctctgccgc tgcggcggtg
    19501 ctctgacgcg ggtcacgcgg caacgcttcg taatacgccc agtttttttc cgcctgtttc
    19561 agcgaaatac tggcactctg catcagctca gcaacggttg aaccgctacc aatattattc
    19621 tgatccatca tgtagcggat acccgcgcgg ttgagggtgt tacgcgtctg caacaacgcg
    19681 acccagctgc cattcagcgt ggattgctgc tggcgaatgg tttgtaaaac agtgaaattt
    19741 tctttgtcat tctttaaggc attaaagaac agaccgcctg atgtcagttg taaaaggcca
    19801 aaaacagcca aaaccagcag taagctggtc acgatcttga tacgttttaa catggtttct
    19861 ctttcctgtg gaccgatgca agattttcgg cctggaaagc aaaaacttta tgaaaaggtg
    19921 taacccgcca tccatggaaa aaccaggcgc tggttatcag atactaaaaa gttattcatt
    19981 atcgcccggg atgtcttctt gtcgggaatt gcccagagca ataaattcct ctaacagatg
    20041 ggttaactgt tgcattttct cggcagtaaa ctgagcttca atctgccgat aggcttcttc
    20101 aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct ttggttagcg agatatagag
    20161 cttccgctga tcattgatcg gctttaatcg caacactaaa ccgtcgcgtt ccatccgcgt
    20221 aaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga tacgccagat cgtgaaaatc
    20281 cattgatggg ctttccgcca gaatacgcac gatgcgccac tgttgctcag tcaaattatg
    20341 ccgtttaacg attgggcgaa aataactcat cgccgcttct cgcgcctgga gcaacgcaat
    20401 ggttagagag tcgtgcatta tctttcccct gaaaaattca aaatcattaa tatagaaaca
    20461 gttaatcatc acgttaccgg gactgcgctg cccctgc
//
LOCUS       DAZGAN020000081        20489 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGAN020000081 DAZGAN020000000
VERSION     DAZGAN020000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.081"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(255..413)
                     /locus_tag="ACHKYO_RS16605"
                     /old_locus_tag="pgaptmp_003321"
                     /pseudo
     CDS             complement(255..413)
                     /locus_tag="ACHKYO_RS16605"
                     /old_locus_tag="pgaptmp_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
     gene            562..687
                     /locus_tag="ACHKYO_RS16610"
                     /old_locus_tag="pgaptmp_003322"
                     /pseudo
     CDS             562..687
                     /locus_tag="ACHKYO_RS16610"
                     /old_locus_tag="pgaptmp_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(747..1253)
                     /gene="yciE"
                     /locus_tag="ACHKYO_RS16615"
                     /old_locus_tag="pgaptmp_003323"
     CDS             complement(747..1253)
                     /gene="yciE"
                     /locus_tag="ACHKYO_RS16615"
                     /old_locus_tag="pgaptmp_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU4430112.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1299..1799)
                     /gene="yciF"
                     /locus_tag="ACHKYO_RS16620"
                     /old_locus_tag="pgaptmp_003324"
     CDS             complement(1299..1799)
                     /gene="yciF"
                     /locus_tag="ACHKYO_RS16620"
                     /old_locus_tag="pgaptmp_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU4430113.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(1885..2064)
                     /gene="yciG"
                     /locus_tag="ACHKYO_RS16625"
                     /old_locus_tag="pgaptmp_003325"
     CDS             complement(1885..2064)
                     /gene="yciG"
                     /locus_tag="ACHKYO_RS16625"
                     /old_locus_tag="pgaptmp_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGU4430114.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(2445..3251)
                     /gene="trpA"
                     /locus_tag="ACHKYO_RS16630"
                     /old_locus_tag="pgaptmp_003326"
     CDS             complement(2445..3251)
                     /gene="trpA"
                     /locus_tag="ACHKYO_RS16630"
                     /old_locus_tag="pgaptmp_003326"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGU4430115.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(3251..4444)
                     /gene="trpB"
                     /locus_tag="ACHKYO_RS16635"
                     /old_locus_tag="pgaptmp_003327"
     CDS             complement(3251..4444)
                     /gene="trpB"
                     /locus_tag="ACHKYO_RS16635"
                     /old_locus_tag="pgaptmp_003327"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGU4430116.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(4456..5817)
                     /gene="trpCF"
                     /locus_tag="ACHKYO_RS16640"
                     /old_locus_tag="pgaptmp_003328"
     CDS             complement(4456..5817)
                     /gene="trpCF"
                     /locus_tag="ACHKYO_RS16640"
                     /old_locus_tag="pgaptmp_003328"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HGU4430117.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGTGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(5818..7413)
                     /gene="trpD"
                     /locus_tag="ACHKYO_RS16645"
                     /old_locus_tag="pgaptmp_003329"
     CDS             complement(5818..7413)
                     /gene="trpD"
                     /locus_tag="ACHKYO_RS16645"
                     /old_locus_tag="pgaptmp_003329"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HGU4430118.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(7413..8975)
                     /gene="trpE"
                     /locus_tag="ACHKYO_RS16650"
                     /old_locus_tag="pgaptmp_003330"
     CDS             complement(7413..8975)
                     /gene="trpE"
                     /locus_tag="ACHKYO_RS16650"
                     /old_locus_tag="pgaptmp_003330"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGU4430119.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDYLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(8997..9090)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(9067..9111)
                     /gene="trpL"
                     /locus_tag="ACHKYO_RS16655"
                     /old_locus_tag="pgaptmp_003331"
     CDS             complement(9067..9111)
                     /gene="trpL"
                     /locus_tag="ACHKYO_RS16655"
                     /old_locus_tag="pgaptmp_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HGU4430120.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            9249..10130
                     /gene="rnm"
                     /locus_tag="ACHKYO_RS16660"
                     /old_locus_tag="pgaptmp_003332"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             9249..10130
                     /gene="rnm"
                     /locus_tag="ACHKYO_RS16660"
                     /old_locus_tag="pgaptmp_003332"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HGU4430121.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10127..10747
                     /gene="yciO"
                     /locus_tag="ACHKYO_RS16665"
                     /old_locus_tag="pgaptmp_003333"
     CDS             10127..10747
                     /gene="yciO"
                     /locus_tag="ACHKYO_RS16665"
                     /old_locus_tag="pgaptmp_003333"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGU4430122.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            10848..11720
                     /gene="rluB"
                     /locus_tag="ACHKYO_RS16670"
                     /old_locus_tag="pgaptmp_003334"
     CDS             10848..11720
                     /gene="rluB"
                     /locus_tag="ACHKYO_RS16670"
                     /old_locus_tag="pgaptmp_003334"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HGU4430123.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(11760..12350)
                     /gene="cobO"
                     /locus_tag="ACHKYO_RS16675"
                     /old_locus_tag="pgaptmp_003335"
     CDS             complement(11760..12350)
                     /gene="cobO"
                     /locus_tag="ACHKYO_RS16675"
                     /old_locus_tag="pgaptmp_003335"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGU4430124.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(12347..13105)
                     /gene="yciK"
                     /locus_tag="ACHKYO_RS16680"
                     /old_locus_tag="pgaptmp_003336"
     CDS             complement(12347..13105)
                     /gene="yciK"
                     /locus_tag="ACHKYO_RS16680"
                     /old_locus_tag="pgaptmp_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HGU4430125.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDASSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            13325..14374
                     /gene="sohB"
                     /locus_tag="ACHKYO_RS16685"
                     /old_locus_tag="pgaptmp_003337"
     CDS             13325..14374
                     /gene="sohB"
                     /locus_tag="ACHKYO_RS16685"
                     /old_locus_tag="pgaptmp_003337"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HGU4430126.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(14410..14661)
                     /gene="yciN"
                     /locus_tag="ACHKYO_RS16690"
                     /old_locus_tag="pgaptmp_003338"
     CDS             complement(14410..14661)
                     /gene="yciN"
                     /locus_tag="ACHKYO_RS16690"
                     /old_locus_tag="pgaptmp_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HGU4430127.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            15041..17638
                     /gene="topA"
                     /locus_tag="ACHKYO_RS16695"
                     /old_locus_tag="pgaptmp_003339"
     CDS             15041..17638
                     /gene="topA"
                     /locus_tag="ACHKYO_RS16695"
                     /old_locus_tag="pgaptmp_003339"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGU4430128.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKVRYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            17848..18822
                     /gene="cysB"
                     /locus_tag="ACHKYO_RS16700"
                     /old_locus_tag="pgaptmp_003340"
     CDS             17848..18822
                     /gene="cysB"
                     /locus_tag="ACHKYO_RS16700"
                     /old_locus_tag="pgaptmp_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HGU4430129.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            19141..19281
                     /gene="ymiA"
                     /locus_tag="ACHKYO_RS16705"
                     /old_locus_tag="pgaptmp_003341"
     CDS             19141..19281
                     /gene="ymiA"
                     /locus_tag="ACHKYO_RS16705"
                     /old_locus_tag="pgaptmp_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HGU4430130.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            19284..19451
                     /gene="yciX"
                     /locus_tag="ACHKYO_RS16710"
                     /old_locus_tag="pgaptmp_003342"
     CDS             19284..19451
                     /gene="yciX"
                     /locus_tag="ACHKYO_RS16710"
                     /old_locus_tag="pgaptmp_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HGU4430131.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            19565..19660
                     /gene="ymiC"
                     /locus_tag="ACHKYO_RS16715"
                     /old_locus_tag="pgaptmp_003343"
     CDS             19565..19660
                     /gene="ymiC"
                     /locus_tag="ACHKYO_RS16715"
                     /old_locus_tag="pgaptmp_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HGU4430132.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            19824..>20489
                     /locus_tag="ACHKYO_RS16720"
                     /old_locus_tag="pgaptmp_003344"
     CDS             19824..>20489
                     /locus_tag="ACHKYO_RS16720"
                     /old_locus_tag="pgaptmp_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HGU4430133.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLG"
BASE COUNT         5090 a         5035 c         5244 g         5120 t
ORIGIN      
        1 gtggtgtcta cccaaaaagc aacctgtgaa tattaggctg gttaagtgcg tattttcatg
       61 gaaggagatc acccaatagc cccgatctcc tttatgttaa tacgcattaa gcgggtaaaa
      121 caggaagatt aagaaaggga gagtttttcg actccccaac caagagaagc agcttataac
      181 taccagatag ttgttatgtc atacgtgacg gataatgtca taaaaaatat gtcattgccg
      241 tgattataat cgcgttattg gataaagcag tcttcagcgg tttcccaggc ttcctgcagc
      301 gtttcctgta caaattcgtg cgcggattct ttatttacaa ctcttaaaat ggtgagtcca
      361 tcattgctgg tggttctgac aatcacgtcc acatggtcat atccgttatt tatggaatga
      421 ctataagggg ggataagcct ctgcaaaaca aaaaaagact ttaacaaaat caatcagtta
      481 aaaacacaga aaaacggtat gaaaaatcac cataaaagtt tgtttttcct tttataaata
      541 gctgcttatc acatttttta taatctacca gtctggtaca tggatatcga taccaccgcc
      601 aattataagc taggcggtgc acagcaacac gatagcgtac gcctcgatcc gtgggtgttt
      661 atgttctcag caggatatcg tttttaattc cacaaaaaaa cgaccccgta atatacgggg
      721 tcaataagga catagcataa agtgtattat ttcttcgctt ctacgccgtc agtttcagag
      781 cgaattaaaa atttctcagt tgtttgcgga atatgctgaa tcagccagtc ggccatttgc
      841 ttttcctcat ttaaaatcgc ttcgatggtt ggaattgaag ctgtatcacc ggcatttttt
      901 gctgctgcta atagtgaggt gtaacaggcg atttcaaatt gctcgaagac atatccgcta
      961 atagagcctt tgactatttc atcagaaggg aatataccac cgattgactg cccaagcgca
     1021 gccattttac tcatggaatc tttaatgact gaacgtgaaa tgtcattacg atcaagaata
     1081 gtttccaatt gaacaatctg gtttttggtt tcactaagat gttgttcaat acgagcgcgt
     1141 agttcaggat aattatctat acggctggcc atggattcaa gcatagattc ggcttgcttt
     1201 tccattgcgt gggcgtcacg taaccagtca tgataatgtt caatacgatt catgaaaatg
     1261 tcctctttat aaaatgacta aaagttaaag ttcatatttc aggctttatt ttcggctttc
     1321 ttatttacgt tattaagggc cagatcagtc agtttgatgt cggtggcctt ttcttcttcc
     1381 agggtctctt tcagaagctt cgctgcttta cgataaccta attgttcagc cagcgtcgct
     1441 aatgtcccgt aactggcaat ctcataatgc tcgactttct gtgctgcggc aatcagtgcg
     1501 gcatcacgca cttcgttttt ctcggtactt tcgatgacct cattagcttc ttcaataaga
     1561 ccttccattg ccacacattt catgcgctta attttcagat tcgattctga ttccacaact
     1621 tgatcaatac gttcaatctg tccatgtgtt tcctcgaggt gcgcatgaaa agcctgactt
     1681 aatttttcgt ttgatgttgc tcttgcgagt tttgccagtg cccgggttaa ttgtttttct
     1741 gcgctgtagg tatctgaaag caggtgaata aatacatctt caatggtctt catattcata
     1801 tttttctcca gtgaaatcac tgcgagccat acggcccgca gatgcaatta tctttatcag
     1861 ctgatattaa taattaacgg tgaatcagga tttgcctgat ttattaccac cgctttgttg
     1921 accgcctttt ttacccgctt cagatgcgcg ttgcggatca tttttaaaat taccgccgct
     1981 atgctgaccg cctttacggc ctgcgtcgga tgccttctca cggtcttcgg cgaaatttcc
     2041 tgaaccacca cgatgttcgg ccatgttatt tctcccgttg cgttgcattg tttcattaat
     2101 atgagtgttg tgtgtcgaca ctcattaaaa ttagtcgcta atgagaatta gtcaaattaa
     2161 gcgcaacgag aagatagagg gaaaatatat tttgaggaac attctggata tattaacaat
     2221 tacctgagga ataagtgact tagagaaaat tattgatttt actggtgtta tgttgcggga
     2281 ttaatttgtt tgattatatt actgttgggc ggaaaaatga cgtaagttga agttcgaccg
     2341 gggtaagcga aacggtaaaa agataaatat taaatgaatt taggattttt ccggcttcat
     2401 taaagaaagt taaaatgccg ccagcggaac tggcggctgt gggattaact gcgcgtcgcc
     2461 gctttcatcg gttgtacaaa aactttcagt gccgccagca ttttctctgg ctcattaata
     2521 tgttgctcga tgattttaac aatggccgaa ccagaaatcg cgcccgcagc tcctgcatca
     2581 atcgctgctt ttacctgatc cggggcggaa ataccaaacc cctgcaatgg aggtgcagcg
     2641 ttgtactctt tcagcttcgc aaccagatga ttgaggggta acgcggcgcg gttttctgcg
     2701 ccggtcacgc ccgctcgtga cagcaaatag gtgtaaccac gaccgtaaga ggctatctgg
     2761 cgcagcaggt cgtcatcggc atttggcggg cagatgaaga taggtgcgac attatggcgc
     2821 aacgcggcct ggcggaaggg cgcggactct tcaattggca catcggcaac cagcaccgaa
     2881 tcgacgccga ctttctcgca ctgggcataa aactcatcaa tgcctttgtt aaacaccaga
     2941 ttggcataca tcaacaggcc aatgggaatg gtcgggtgtt tctggcgaat cagtgccagc
     3001 atttcaaaac attgtgccgg agtcacacct gccgcaaagg cgcgcagagt ggcgttttga
     3061 atggtcgggc catccgccag tgggtcggag aaggggatac ctaactccag cgcgtcagca
     3121 ccggcttcaa ttagcgtatc gataattttc aatgactgct caatgcccgg atcaccgagc
     3181 gtgacgaaag gaacgaatgc gccttctttg cgctccttca actgggcaaa cagagattcg
     3241 tagcgttcca tcagatttcc cctcgtgctt tcaaaatatc gtgaacggtg aagatgtctt
     3301 tatcgccgcg accggaaagg ttaaccacca gtagctgctc tttatccggg ttttcgcgca
     3361 tcattttcaa cgcatgggcc agggcgtggg aggattccag cgccgggatg atcccttcgt
     3421 gcaggcacag cgttttgaag gcttcaaggg cttcatcatc ggtaatagac acgtaatcag
     3481 cgcgtccagt gctgttaaga tacgcgtgtt gtgggccgac agacgggaaa tccagtccgg
     3541 cggagatgga gtaagattct tcaatctgcc cgtcttcggt ttgcatcatc ggcgctttca
     3601 taccgaaata gatacccacg cgaccatgtt ttagcggtgc gccgtgctcg ccagtttcga
     3661 taccgtgacc acctggctcc acaccaatca ggccgacgtt ggtttcattg atgaaatcag
     3721 caaacatgcc gatggcattc gaaccgccgc caacacaggc gataacggca tccggcaggc
     3781 gaccttctct ttccagaatc tgcgctttgg tttcttcgcc aatcatccgc tgaaactcac
     3841 gcacaatggt cggataagga tgcgggccag ctgcggtgcc cagcatatag tgcgcggttt
     3901 cgtaactacc ggaccagtcg cgcagcgcct cgttacaggc atctttcagc gtcgcggaac
     3961 cgctatgcac cgggatcact tccgcaccca ttaagcgcat acgaaaaacg ttaggcgact
     4021 ggcgttcaac gtctttggca cccatataaa tacggcattt caggccgagc agggcgctgg
     4081 cgagggccga cgccacgcca tgctgaccgg caccggtttc ggcgatgatt tcggttttac
     4141 ccatccgctt cgccagcaac gcctgcccca gcacctggtt agttttatgc gcgccgccgt
     4201 gcagcaaatc ttcacgcttg agatacagcg tggtgttcgt cccggctgta atgttctggc
     4261 atttggtcag cgcggttgga cgcccggcat agtttttcag caggtcgttg aactgagcct
     4321 gaaattcagg atctttttgc gcactgacaa aagcttcttc cagctggcgc agagcaggca
     4381 tcaggatttg tggcacgtac atgccgccaa actcaccaaa ataggggtta agtaatgttg
     4441 tcattgttcc tttccttaat atgcgcgcag cgtctggaaa accgaggcca aaagacgtgc
     4501 gtctttgatg cccggttgcg actctacagc agaattaaaa tcaagtccgg cgcagccggt
     4561 ttgtgccgct tccacgcagt tatctgcgcc taagcccccc gccagcagaa cgttgccaag
     4621 cgattgacca tttaatagtg accagtcaaa acgttgcccg gttccaccct ggccgttgtc
     4681 taaaacatat ttatcaacgt gctgaaactc gcgggcgggc agggtttcac cgacgcttaa
     4741 tgctttccag atggcaacat gtgctggcag agcttcacgc agcgtatcga tatacagctg
     4801 ttcttcatta ccatgcagtt gcactgccgc cagcgataac accttagctt tgtccaccac
     4861 atcggcaata tcgtgattgc ggaacacgcc aacatactgc aacggtgccg cagccatcac
     4921 ttcctgcgcc tgttcaacgt tgacgcaacg cggtgatgtc gcaacaaaaa tcaacccacc
     4981 gtaaatcgcg cccgcgtcat aagctgcttt agcatcttgc ccacgcgtca ggccacatac
     5041 tttattctca cccagcaaca cccggcgcac ggcggcgtgc aaatcgtcat gggccatcaa
     5101 cgccgaacca atcagaaaac cgttagcgaa gtggcttaac tcgcgcacct gagcgtaagt
     5161 attgatgccg gattcgctga ttaccgtcac gttgtgcccc agtttcggcg caagctcgcg
     5221 ggtacggttg agatcaatcg acaaatcacg cagatcgcgg ttgttgatgc caacgacctt
     5281 tgcccccaat gcaatggcac gctccagttc ctcttcatta ctgacttcgg tcagcacacc
     5341 catctccaga ctgtgggcga cggcggcaag ctggcgatat tgttcgtcat ccagtactga
     5401 aagcattaat aagcaggcat cagcctggta atagcgcgcc agatagatct ggtaaggatc
     5461 gataatgaag tctttacata aaatcggctg cggggcgatt tggctgacga tggggaggaa
     5521 atcaaagctc ccctgaaaat atttctcatc agtcagcacc gaaattgccg aagcgtaatg
     5581 tttataaatg gcggcaatgc gtgctggatc gaaatcatca cggatcacgc cttttgacgg
     5641 cgacgctttc ttgcactcca gaataaacgc cgtgcgcgca ccctgtagcg catcataaaa
     5701 atgtcgcgtg ctcggctgaa cctcattctg aaaactggcc agcggttgct gctgtttgcg
     5761 ggtttctacc caaatcgcct tgtctgcgac gattttcgct aaaacggttt gcatcattta
     5821 ccctcgtgcc gccagtgcgg taactctgtc gtaagcggaa ccactgcgca gtacctcaag
     5881 aacggtttgc gcattggctt gcagatcttc atggccatgc aggcgcatta acatggcgac
     5941 gttcgcagcg acggctgctt catgggcggc gtcgccttta ccttgtaaca agcgtgttaa
     6001 aatgtcacgg ttttcttccg gtgttccgcc tgccagttgc tcctggtggt agggagtcag
     6061 gccaaaatct tcagcggtca attgatagct cttaatttcg ccgtcatgca gctcggcaac
     6121 gattgtcggc gcgtgtaatg aaacttcatc catcccgccg ctgtgcacca ccgccgcgcg
     6181 ttgatacccc agcacgcgca aggtttcggc aatcggcagc accagttccg gactataaac
     6241 accaattaac gccagcggcg gatgcgccgg gttaatcaat ggccccagca cattgaacag
     6301 ggtgcgggtt ttcagttgct ggcgaaccgg catcgcgtgg cggaatccgg tgtgatactt
     6361 cggcgcaaag aggaaacata cacctaactc atccagcgcc tggcgcgatt tatcggcgtt
     6421 catatcaaga ttaataccga acgccgccag cagatcggac gaaccagatt tactggagac
     6481 gctacggttg ccgtgtttcg ccactttcag cccacaggcc gcggcgacaa acgcactggc
     6541 ggtagaaata ttgatactgt tgctgccgtc accgccagta ccgacgatat cagcaaacag
     6601 ataatccggg cgcgggaacg gcgcggcgtt ttccagtagc gcggttgctg ctccggcgat
     6661 ctcgttcggg tgctcaccgc gaattttcat gctcaccagc gccgccgcca gttgttccgg
     6721 cttcagctcg ccacgcacca ccgctgaaaa cagctggtgg ctttcttgtt ggctaagcgt
     6781 ctgcgcctga tacagttttt ccagaatcgg ttgcagcgtg ttggctggct ctagtttctg
     6841 ctgcgcccag gccagcgttt gttccagcag gcgagcgccc tgggtggtga gaatggattc
     6901 cggatggaac tggaatccac aaacgcgatc cgcatcgtga cgtactgcca tcaccatgcc
     6961 attaaaatgg gcgttgatgg ttaaaccggc cggaatgtta ctgccaacca gcgagtgata
     7021 acgcgccacc ggcagcgggt ttgttaatcc ggcaaacatc gcctgaccgt catgttcaat
     7081 gctcgacgct ttaccgtgaa gaatttcgcc cgcctgaccg acatagcccc cgtaagcttc
     7141 gacaattgcc tgatgtccga ggcaaatgcc aataatgggc agcttgccac gcaagcgggt
     7201 gaggagttcc ggcatacaac cggcttcgct cggcacaccg gggccaggag aaagcatcag
     7261 taccggattg ctcatggtcg ccaggcgttc aattaaggtc tgcgccggaa tatggttgcg
     7321 gtaaatcacc acgttatgcc cattgctgcg caactgatct gccaggttgt acgtaaaaga
     7381 gtcgatatta tcgagcagca gtatgtcagc catcagaaag tctcctgtgc atgatgcgcg
     7441 gtggcaatag cgcgcagtac agcgcgggct ttattacggg tttcgtcggc ttccgactgc
     7501 ggaatagaat caaggactac gccagcaccg gcttgcacgg tggcgatacc gttttccacc
     7561 agcgccgagc ggatcacaat gcaggtgtcg agatcgccgt gcgcggtgaa ataacctacc
     7621 gcgccgccgt agctgccgcg gcgacgacct tccgcctcgg caattaactg catggcgcgc
     7681 actttcggcg caccgcttaa cgtccccata ttcatacagg cgcggtaagc gtgtagggcg
     7741 tcgagatcgt ggcgcagttc accgaccacg cgggagacca ggtgcatcac gtaagagtaa
     7801 cggtcaactt tggtgaggtc ggcgacgtag cggctgccgg gggtgcaaat gcgtgccaga
     7861 tcattacggg caagatccac cagcatcaga tgttcagaaa gctctttatg atcggtacgc
     7921 atttccagtt cgatgcggct gtcgaggtct ctgtccagcg agccatcggc acgacgaccg
     7981 cgtggacgtg ttccggcaat cgggtagatc tcaatctggc ggctggtggc gtcatacttg
     8041 agcgaacttt ccggcgacgc gccaaacagg gtgaaatcat tatcctgcat aaaaaacatg
     8101 tacgggctgg gattactctt tttcagcacg taataggccg ccagcggtga cgggcagggc
     8161 agagagaaac ggcgagatgg caccacctgg aaaatttctc cagcgcgaat cgctttttgc
     8221 aacaaacgca ctacgccacc gaactcttca tcgctctgat tacattcaca acgcatatgc
     8281 ggcacggaaa ccaccggcag cggcggcgcg gcttcggtca gttgctgacg tagttcgttc
     8341 aggcgagcag tgagacgttg tttttcttct tcattcggag caaacaggct ggcctgaata
     8401 cgggtgcttt ttttctgatg gtcaatcacc atcagcgttt cagcgagata aaaacagaaa
     8461 tcagggcagt tattttccgc tgacagttgc ggtaaatctt caaatcccgc cacaaggtca
     8521 taagagaaca ggccgccgaa gaacattgct tctcgttctt ccttcggtac attcaacaga
     8581 ttctgcaata aacggaaagc gtcaaaaacc gaaagggagc ataagcgagc gtcttcatcc
     8641 agcagtggac tgacaggggg gaagcgcagc acacggcagt ttggtaattg ttcattttcc
     8701 acacccgcag gcagggcgtt atccagtagt gtcagcaggg cttcgccgtt gccggaaagt
     8761 gcctggattg tgacagtgtc acctaaagct gtaatgcgca gcgcactgtc taccagcagc
     8821 aggcttttta aataatcttt gctgtcgata tctgcggatt ccagcagcag cgttgccgga
     8881 cgatccccac acaactggtg aaaaagcgcg gtgggattgt cgcgataagc gccttcgcag
     8941 gttagcagtt cgagagtcgg tttttgtgtt tgcattgtta ttctctaatt ttgttcaaaa
     9001 aaaagcccgc tcattaggcg ggctgggtat ctgattgctt tacgcatggt gaatacactg
     9061 cccgtttcag gaagtgcgcc accaaccttt cagtacgaaa attgctttca ttgtcgatac
     9121 cctttttacg tgaacttgcg tactagttaa ctagttcgat gattaattgt caacagctca
     9181 tttcagaata tttgccagaa ccgttatgat gtcggcgcaa aaaacattat ccagaacggg
     9241 agtgcgcctt gagcgacacg aattatgcag tgatttacga cctgcacagc cataccacag
     9301 cttccgatgg ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgag atgcgcgtcg
     9361 gcaccctggc gatcaccgac catgacacca cagctgcaat cgcgccagca agagaggaaa
     9421 tttcacgttc gggactggcg ttgaatctta ttcccggcgt ggaaatttcc acggtctggg
     9481 aaaatcatga aattcatatt gtcgggctga atattgatat tactcatccg ctaatgtgtg
     9541 agttcctcgc gcagcagaca gaacggcgca atcagcgggc gcagctgatt gccgaaagac
     9601 ttgaaaaagc gcaaatacct ggggcgctgg aaggcgcaca acgactggcg cagggcggcg
     9661 cagtgacgcg cggtcatttt gcgcgtttcc ttgttgagtg cggcaaagcc agttcaatgg
     9721 cggatgtctt taaaaagtat ctggcgcgcg ggaaaaccgg atacgttccg ccacagtggt
     9781 gtacaataga acaagctatt gatgtcattc atcattctgg cggtaaggcg gtgctagctc
     9841 atcctgggcg gtacaatctt agtgctaaat ggctgaaaag attggttgcg cattttgccg
     9901 aacaccacgg tgacgcgatg gaagtcgcgc agtgccagca atcgcccaat gaacgtaccc
     9961 agctggcggc ccttgcgcgt cagcatcatt tatgggcatc acaaggatct gattttcatc
    10021 agccatgccc gtggatcgaa ctgggtcgta aactctggct gcccgcaggc gttgaaggcg
    10081 tctggcagct atgggaacag ccgcagaaca ccacagagag ggaattatga gccagttttt
    10141 ttatattcat cctgataacc cacagcaacg tctgatcaac caggcggtgg agatcgtgcg
    10201 taaaggcggg gtgattgttt atccaactga ttccggctat gcgctcggct gtaaaattga
    10261 agataaaaac gcgatggagc gtatttgtcg tattcgccag ctgccggacg gtcacaactt
    10321 taccctgatg tgtcgcgatc tttctgaact ctcgacctat tcatttgttg ataacgttgc
    10381 gtttcgttta atgaaaaaca acacgccggg caactatacc ttcatcttga aggggacgaa
    10441 ggaagtgcca cgccgtctgt tgcaggaaaa acgcaaaacc atcggtatgc gtgtgccttc
    10501 taacccgatt gcgcaggcgt tgcttgaagc actgggcgaa ccgatgcttt ccacttcgct
    10561 aatgctgcca ggcagcgaat ttaccgaatc cgatcccgaa gaaattaaag accgtctgga
    10621 aaaacaggtg gatttgatta tccacggcgg ctatctcgga cagaaaccga caacggttat
    10681 tgatcttacc gatgatacgc cggtcgtggt gcgtgaaggc gtaggtgatg tgaagccttt
    10741 cttataagtg cgtaactctg tatactacgc ggccttaaaa tggcgctata cggcgccatt
    10801 gattctcctg ttcgacgcct gtgaaggcga caccaaaagg aagctctatg agcgaaaaat
    10861 tacagaaagt gctggcccgc gctggccacg gctcccgccg tgaaatcgaa tctattattg
    10921 aagccggtcg tgtgagtgtt gatggcaaaa ttgctaaact cggcgatcgc gttgaagtta
    10981 cccctgggct gaaaattcgt atcgatggtc atctgatttc ggtacgtgag tccgctgaac
    11041 aaatttgtcg cgttctggcc tattacaaac cggaaggtga gttgtgtacc cgtaacgatc
    11101 cggaagggcg tccgacggtg tttgaccgtc tgccaaagtt gcgtggcgca cgctggattg
    11161 ccgtaggtcg tctggacgtt aacacctgtg gtctgctgct gttcaccacc gatggtgaac
    11221 tggcaaaccg tttaatgcat ccaagccgtg aagttgaacg tgaatacgcc gtgcgtgtat
    11281 ttggtcaggt tgacgacgcg aaactgcgtg atttgagtcg tggcgtgcaa ctggaagatg
    11341 gtccggcagc ttttaaaacc atcaagttca gcggcggcga agggatcaac cagtggtaca
    11401 acgtgactct gaccgaaggc cgtaaccgtg aagttcgtcg tctgtgggaa gcggttggtg
    11461 tgcaggtgag ccgcctgatc cgtgttcgtt acggtgatat cccactgcca aaaggtctgc
    11521 cacgcggtgg ctggacggag ctggacctcg cccagactaa ctatctgcgc gaactggtgg
    11581 agcttccgcc agaaaccagc tctaaagtcg ctgtagaaaa agaccgtcgt cgcatgaagg
    11641 ctaatcagat tcgtcgtgcg gtgaaacgtc acagtcaggt gagcggcggt cgtcgttctg
    11701 gcgggcgtaa taacggttaa tcaaagtatg ccggacgtca tatccggcat ttttacagat
    11761 taataatcaa tccctatctg cgcttttaca ccggcatcaa acgcatgttt gacggggcgt
    11821 aattcactta ccgtatctgc cagttcaaga atatcccgat gacaaccacg acccgtgata
    11881 atcaccgtct gttgatgtgg acgttcattt aacgcctgca ccacttcttc cagtggcaaa
    11941 tagtcatacg ccaccatata cgtcagttca tcaagcaaaa ccatatccag tgaggaatca
    12001 gcaagcatcc gctttgcatg ttgccagact tcgcggcagg cggcggtatc agactcgcgg
    12061 ttttgtgtat cccaggtaaa gcccgttgcc atcacctgaa actcaacgcc atgtggctcc
    12121 agcagattgc gttcgccatt aggccaggtg cctttaataa actgcacgac gcctactttt
    12181 tttccgtgac caactgcgcg tgttgccgta ccaaatgccg cggtggtttt gccttttcca
    12241 ttgccggtaa agacgatgat aataccgcgt tcatcctggg cctgggccac acgagcatct
    12301 actttttctt tcactcgctg ctgacgctgt tggtagcgtt catcactcat tgggaaattc
    12361 ctggtttacg gcccggttgg gcgtcaaagg tcatgccggt tttacggcgg ctgtcatcgc
    12421 ccatcagcca gaggtagagc ggcatgatat cagcgggtgt tttaagtttc tgtggatctt
    12481 cggtcgggaa ggcgcttgcg cgcattgcgg tgcgcgtacc gcctgggtta atgcagttga
    12541 cgcgcaggcg ctgctgatat tcatcggcca gtacctgcat catcccttcg gtggcaaatt
    12601 tcgacgctgc atatgcaccc cagttagctc gtccctgacg tccaacgctt gatgaagtaa
    12661 agaccagtga actggcgtcc gatttgagta ataaaggaag cagtgcctgg gtgagcataa
    12721 aggtggcatt aacgttgacc tgcatgacgt cctgccagac ctgcggattt tgttcgctca
    12781 ttgggcaaat atcgccgagc aatccggcat tatgcaaaac accatccaga cgcggataat
    12841 taacgacaat gcgctgtgcc agttgttggc aattttcgga cgtgcaggtc agcaaatcga
    12901 gaataaacca ctgtggctga cgcccagttt cttcgtttat gtggctggct acctgacgta
    12961 atttttcttc attacggccc aacagaatca ctgtcgcacc atagcgtgca tacgtcatcg
    13021 cggcttcacg accaatccca tcgctggctc ccgtcaccag gataatgcga tcattgagta
    13081 aatcttgttt tggctggtaa tgcatggcta ctcctcaacg acgttgtctg tcgcgctgcc
    13141 agtgtacttt atgactttgg ggctttatgc ctgaaacaga agggcatttc aatcagcctg
    13201 gggcgataac accgcaaaat ggatactttg tcatactttc gctgcaataa catctctgcg
    13261 agacggctta acatgcctgt tgtaaactgt gagccaaagc gttgtttaac caaggtgggg
    13321 actcgtggaa ttgttgtctg aatatggttt gtttttggcg aaaatcgtta ccgttgtgct
    13381 agcgattgcg gcgattgccg ccattattgt caatgttgct caacgtaata aacgccagcg
    13441 tggcgagtta cgggtcaaca atctcagcga acagtataag gagatgaaag aagaactggc
    13501 cgcggcgctg atggactcac atcagcaaaa acagtggcac aaagcgcaga agaaaaagca
    13561 caagcaagaa gcgaaagcag caaaagcgaa agccaaactg ggcgaggtgg caactgacag
    13621 taaacctcgc gtctgggtgc tggattttaa aggcagcatg gacgcccatg aagtgaactc
    13681 gctacgtgaa gagataacgg ctgtactcgc agcattcaaa ccgcaggatc aggttgtgct
    13741 acgtctggaa agccctggtg gcatggtgca tggttacggg ttggcggctt cgcagctgca
    13801 gcgtctgcgt gataaaaaca ttcctttaac tgttacggta gacaaagtcg ctgccagcgg
    13861 cggttacatg atggcctgtg tggcggacaa aattgtttcc gcaccgtttg ctattgtggg
    13921 ttccattggc gtggtggcgc aaatgcccaa ctttaaccgc ttcctgaaaa gcaaagatat
    13981 tgatatcgaa ctgcacactg ccgggcagta taagcgtact ctgaccttgc tgggtgaaaa
    14041 taccgaagaa gggcgggaga aattccgcga agagttgaac gaaacgcatc agttgtttaa
    14101 agattttgtg aagcgtatgc gtccgtctct ggatattgaa caggtggcaa cgggtgaaca
    14161 ctggtacgga caacaggcgg tagagaaagg cctggttgat gaaatcaaca ccagtgatga
    14221 agttattctt agcctgatgg aaggccgtga agtggttaat gtacgctata tgcagcgtaa
    14281 acgactcatt gaccgattca ccggcagcgc ggcagagagc gccgatcgat tgttgctacg
    14341 ctggtggcag cggggtcaaa agccattgat gtaaaagaca aacgcgaggc taagacctcg
    14401 cgttttgctt taatcaacca gatgatattt ttctgaaagc acatgggcca ggtgtttgaa
    14461 catattaaac accgcggtgc ttttggctgt tggcaatcct tgttcatcta aaaagtagtc
    14521 gccggtaaat accagcacgc cattacgctg cgtgacgccg gtggcttcaa tccctgcgag
    14581 cgtatcctca tgctcacgaa tgattttgtt ggcctctttc aacagcgttt cgcggtcgat
    14641 gggttgtgtc tctttgttca ttatttactc cttaaacaag gacattagtc tacgccaggc
    14701 atggcttgca gacaaatata ccacgctggt ggcaagagcg ccttactggt aactttggat
    14761 tttgcatgct aataaagttg cgtatcggat tttatcaggt acagtgtgac gctttcgtca
    14821 atctggcaat agatttgctt gacattcgac caaaattccg tcgtgctata gcgcctgtag
    14881 gccaagacct gttaactcag tcacctgaat tttcgtgaac agagtcacga caaggggttg
    14941 atatccgcag agaacgagtc catatcggta actcgttgcc agtggaaggt ttatcaacgt
    15001 gcgacgcatt cctggaagaa tcaaattagg taaggtgaat atgggtaaag ctcttgtcat
    15061 cgttgagtcc ccggcaaaag ccaaaacgat caacaagtat ctgggtagtg actacgtggt
    15121 gaaatccagc gtcggtcaca tccgcgattt gccgaccagt ggctcagctg ccaaaaagag
    15181 tgccgactct acctccacca agacggctaa aaagcctaaa aaggatgaac gtggcgctct
    15241 cgtcaaccgt atgggggttg acccgtggca caattgggag gcgcactatg aagtgttgcc
    15301 tggtaaagag aaggtcgtct ctgaactgaa acaactggct gaaaaagccg accacatcta
    15361 tctcgcaacc gaccttgacc gcgaagggga agccattgca tggcacctgc gggaagtgat
    15421 tgggggtgat gatgcgcgct atagccgagt ggtgtttaac gaaattacta aaaacgcgat
    15481 ccgccaggca tttaacaaac cgggtgagct gaatattgat cgtgttaatg cccagcaggc
    15541 gcgtcgcttt atggaccgcg tggttgggta tatggtttcg ccgctgctat ggaaaaagat
    15601 cgctcgtggt ctgtctgccg gtcgtgtgca gtcggtggcg gttcgcctgg tggtcgagcg
    15661 tgagcgtgaa attaaagcgt tcgtgccgga agagttctgg gaagtcgatg ccagcacgac
    15721 cacgccatct ggtgaagcgt tggcgttaca ggtgactcat cagaacgaca aaccgttccg
    15781 tccggtcaac aaagaacaaa ctcaggctgc ggtaagtctg ctggaaaaag tgcgctacag
    15841 cgtgctggaa cgtgaagaca aaccgacaac cagtaaacct ggcgctcctt ttattacctc
    15901 tacgctgcaa caagctgcca gcacccgtct tggatttggc gtgaaaaaaa ccatgatgat
    15961 ggcgcagcgt ttgtatgaag caggctatat cacttacatg cgtaccgact ccactaacct
    16021 gagtcaggac gcggtaaata tggttcgcgg ttatatcagc gataattttg gtaagaaata
    16081 tctgccggaa agtccgaatc agtacgccag caaagaaaac tcacaggaag cgcacgaagc
    16141 gattcgtcct tctgacgtca atgtgatggc ggaatcgctg aaggatatgg aagcagatgc
    16201 gcagaaactg taccagttaa tctggcgtca gttcgtagcc tgccagatga ccccagcgaa
    16261 atatgactcc acgacgctga ccgttggtgc gggcgatttc cgcctgaaag cacgcggtcg
    16321 tattttgcgt tttgatggct ggacaaaagt gatgcctgcg ttgcgtaaag gcgatgaaga
    16381 tcgcatctta ccagcagtta ataaaggcga tgctctgacg ctcgttgaac ttacaccagc
    16441 ccagcacttt accaagccgc cagcccgttt cagtgaagca tcgctggtta aagagctgga
    16501 aaaacgtggt atcggtcgtc cgtctaccta tgcgtcgatc atttcgacca ttcaggatcg
    16561 tggctacgtg cgagtagaaa atcgtcgttt ctatgcggaa aaaatgggcg aaatcgtcac
    16621 cgatcgcctt gaagaaaatt tccgcgagtt aatgaactac gactttaccg cgcagatgga
    16681 aaacagcctc gaccaggtgg caaatcacga agcagagtgg aaagctgtac tggatcactt
    16741 cttctcggat ttcacccagc agttagataa agctgaaaaa gatccggaag agggtggtat
    16801 gcgcccgaac cagatggttc tgaccagcat tgactgcccg acttgtggtc gcaaaatggg
    16861 gattcgcaca gcgagcaccg gggtattcct tggctgttct ggctatgcgc tgccgccgaa
    16921 agagcgttgc aaaaccacca ttaacctggt gccggaaaac gaagtgctga acgtactgga
    16981 aggcgaagac gctgaaacca acgcgctgcg cgcaaaacgt cgttgcccga aatgcggcac
    17041 ggcgatggac agctatctca tcgatccgaa acgtaagttg catgtctgtg gtaataaccc
    17101 aacctgcgac ggttacgaga tcgaagaggg cgaattccgc attaaaggtt atgacggccc
    17161 gatcgttgag tgtgaaaaat gtggctctga aatgcacctg aaaatggggc gtttcggtaa
    17221 atacatggcc tgcaccaacg aagagtgtaa aaacacacgt aagattttac gtaacggcga
    17281 agtggcacca ccgaaagaag atccggtgcc attacctgag ctgccgtgcg aaaaatcaga
    17341 tgcttatttc gtgctgcgtg acggtgctgc cggtgtgttc ctggctgcca acactttccc
    17401 gaaatcgcgt gaaacgcgtg cgccactggt ggaagagctt tatcgcttcc gcgaccgtct
    17461 gccggaaaaa ctgcgttatc tggccgatgc gccacagcag gatccggaag gtaataagac
    17521 catggttcgc tttagccgta aaaccaaaca gcaatatgtc tcttcggaaa aagacggaaa
    17581 ggcgactggc tggtcagcat tttatgttga tggcaaatgg gttgaaggaa aaaaataacc
    17641 tttaattctg tcaggttttt ataaacaaag ggtcgcgaaa gcggcccttt tttattgcat
    17701 attatttttt cttcacacct atacactaag gctataaatg atatagtggt tatagttagc
    17761 acctttttta ttattaaatc gtattagtca cccgccaggt gtgacgaaaa aacgatgttc
    17821 tgatggcgtc taagtggatg gtttaacatg aaattacaac aacttcgcta tattgttgag
    17881 gtggtcaatc ataacctgaa tgtctcatca acagcggaag gactttacac atcacaaccc
    17941 gggatcagta aacaagtcag aatgctggaa gacgagctag gcattcaaat tttttcccga
    18001 agcggcaagc acctgacgca ggtaacgcca gcaggacaag aaataattcg tatcgctcgc
    18061 gaagtcctgt cgaaagtcga tgccataaaa tcggttgccg gagagcacac ctggccggat
    18121 aaaggctcgc tgtatatcgc caccacgcat acccaggcac gctacgcatt accaaacgtc
    18181 atcaaaggct ttattgagcg ttatcctcgc gtttctttgc atatgcacca gggctcgccg
    18241 acacaaattg ctgatgccgt ctctaaaggc aatgccgatt tcgctatcgc cacagaagcg
    18301 ctgcatctgt atgaagattt agtgatgtta ccgtgctacc actggaatcg ggctattgta
    18361 gtcactccgg atcacccgct ggcaggcaaa aaagccatta ccattgaaga actggcgcaa
    18421 tatccgttgg tgacatatac cttcggcttt accggacgtt cagaactgga tactgccttt
    18481 aaccgcgcag ggttaacgcc gcgtatcgtt ttcacggcaa cggatgctga cgtcattaaa
    18541 acttacgtcc ggttagggct gggggtaggg gtcattgcca gcatggcggt ggatccggtc
    18601 gccgatcccg accttgtgcg cgttgatgct cacgatatct tcagccacag tacaaccaaa
    18661 attggttttc gccgtagtac ttttttgcgc agttatatgt atgatttcat tcagcgtttt
    18721 gcaccgcatt taacgcgtga tgtcgttgat gcggctgtcg cattgcgctc caatgaagaa
    18781 attgaggtca tgtttaaaga tataaaactg ccggaaaaat aatttcttgc gttattttcg
    18841 gcacctttta tgtagcgaag gtgccggaat atattctctt ttgttactta tttgtatacc
    18901 gtgcacaata tttttatccc tcatatttat agggtaaatt acctgtaaag tagtgtctaa
    18961 ttacccgcct cgcaaattcc agttttcaaa tatctatttc aggtcaaaag attgaattat
    19021 tcactggaga cgattcgtaa attcgctgga tttttgacta aagtttcttt aggatttgtc
    19081 tcatcgtata attaattaca ccaatcgttc aacgtcttgc tgataagtga tggcgattat
    19141 atgaggttag caatgccttc tggaaatcag gaaccccgcc gcgatcctga attaaaacgt
    19201 aaagcctggc tggcggtttt tcttggttct gcacttttct gggtggttgt cgcactgctg
    19261 atttggaaag tgtggggata actatggtcg gtcaggagca actggagtcg tcaccattat
    19321 gccagcatag tgacaatgaa acagaaacga aacgggaatg ttccgtcgtt attccagacg
    19381 actggcaact aacatcgcag cagcaagcct ttatagaact gtttgctgaa gatgatcagc
    19441 cgaaacaata attatcatca ttcttattac ccatttttaa tgaattacag ggcttttaat
    19501 acaccgcagc aataacaggt tgagttatct caacacaaag taataaccgt taagggtgta
    19561 gcctatgatc aacacaaata tgaaatattg gtcctggatg ggcgcatttt ctctgtcgat
    19621 gctcttctgg gccgaactcc tctggatcat tactcactga tccctgaccc cgctgcggcg
    19681 gggttgtcat ttgctttgcc acaaggtttc tcctctttta tcaatttggg ttgttatcaa
    19741 atcgttacgc gatgtttgtg ttatctttaa tattcaccct gaagagaatc agggcttcgc
    19801 aaccctgtca ttaaggagga gctatgtcgt caaccctacg agaagccagt aaggacacgt
    19861 tgcaggccaa agataaaact taccactact acagcctgcc gcttgctgct aaatcactgg
    19921 gcgatatcac ccgtctaccc aagtcactca aagttttgct cgaaaacctg ctgcgctggc
    19981 aggatggtaa ctcggttacc gaagaggata tccacgcgct ggcaggatgg ctgaaaaatg
    20041 cccatgctga ccgtgaaatt gcctaccgcc cggcaagggt gctgatgcag gactttaccg
    20101 gcgtacctgc cgttgttgat ctggcggcaa tgcgcgaagc ggttaaacgc ctcggcggcg
    20161 atactgcaaa ggttaacccg ctctcaccgg tcgacctggt cattgaccac tcggtgaccg
    20221 tcgatcgttt tggtgatgat gaggcatttg aagaaaacgt acgcctggaa atggagcgca
    20281 accacgaacg ttatgtgttc ctgaaatggg gaaagcaagc gttcagtcgg tttagcgtcg
    20341 tgccgccagg cacaggcatt tgccatcagg ttaacctcga atatctcggc aaagcagtgt
    20401 ggagtgaatt gcaggacggt gaatggattg cttatccgga tacactcgtt ggtactgact
    20461 cgcacaccac tatgatcaac ggccttggc
//
LOCUS       DAZGAN020000082        20461 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGAN020000082 DAZGAN020000000
VERSION     DAZGAN020000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.082"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(258..851)
                     /locus_tag="ACHKYO_RS16725"
                     /old_locus_tag="pgaptmp_003345"
     CDS             complement(258..851)
                     /locus_tag="ACHKYO_RS16725"
                     /old_locus_tag="pgaptmp_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430134.1"
                     /translation="MDIEDFLEAEDNDLSLWNDDILSIDWFVILKELLKYQNRLKLNY
                     SELLLLANFVSFHQDVDSQTLPSISLFATRMRTSRDAIQGVLVCLEEKDMLQKISYAQ
                     IDHDDDLRNIYDIKPLIRRLIWLLKSGCDKKHTCPLCGKVAISNEEIEKKFGFRICGN
                     KKRPQSWCRSCRSTKQRRLNSSLIGKKKPRKSPPEVV"
     gene            complement(983..1363)
                     /locus_tag="ACHKYO_RS16730"
                     /old_locus_tag="pgaptmp_003346"
     CDS             complement(983..1363)
                     /locus_tag="ACHKYO_RS16730"
                     /old_locus_tag="pgaptmp_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4430135.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDGTIGKKLQKA
                     EGVVEGMLMLLGVRLEMDRYVQRL"
     gene            complement(1356..1550)
                     /locus_tag="ACHKYO_RS16735"
                     /old_locus_tag="pgaptmp_003347"
     CDS             complement(1356..1550)
                     /locus_tag="ACHKYO_RS16735"
                     /old_locus_tag="pgaptmp_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HGU4430136.1"
                     /translation="MTFTVKTIPDMLLEAYGNQTEVARILNCSRNTVRKYTGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            complement(1547..2110)
                     /locus_tag="ACHKYO_RS16740"
                     /old_locus_tag="pgaptmp_003348"
     CDS             complement(1547..2110)
                     /locus_tag="ACHKYO_RS16740"
                     /old_locus_tag="pgaptmp_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU4430137.1"
                     /translation="MQQRKPKKCKVCGTPFVPFRSYQKVCCGQCALELVRKEKAIASA
                     KEQADKLKARRRDLQPRSYWIKQAQQAVNAYIRERDRNLPCVSCGTFDSAQWDAGHYR
                     TTVAAPQLRFDERNIHKQCVVCNQYKSGNLVPYRVELINRIGQEAVDEIESNHNRHRW
                     TVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            complement(2118..2567)
                     /locus_tag="ACHKYO_RS16745"
                     /old_locus_tag="pgaptmp_003349"
     CDS             complement(2118..2567)
                     /locus_tag="ACHKYO_RS16745"
                     /old_locus_tag="pgaptmp_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HGU4430138.1"
                     /translation="MEIEMIKAANGVFVPAYERDLPRLAKFKNGELYTLEAKLTRNPS
                     FHRKMFAFLNFCFQYWCAEHAGYEFSDEATQFDEFRKNLTILAGFYDVVTTIRGEVRY
                     RAKSLSYANMDQDEFERCYNAMINAALKHVFGRSNSPELNNRLLSFF"
     gene            complement(2567..3538)
                     /locus_tag="ACHKYO_RS16750"
                     /old_locus_tag="pgaptmp_003350"
     CDS             complement(2567..3538)
                     /locus_tag="ACHKYO_RS16750"
                     /old_locus_tag="pgaptmp_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HGU4430139.1"
                     /translation="MQTKKAAVGQWGRIFEYYGMPPVTGVKHYNGPCPICGARGKFRC
                     DDKDGSGSWICVCGHGDGMKLLQLATGKPWVTLCDEIDRLIGNTWKREKVSQPVTEIS
                     RKRKLVMDKFSRLSCLRGTTGEAYLQGRGILQLPTESVRFCDRQIASGREYQAIYAIA
                     TDDKGSLCYLHRTLLDGDRKANVEAAKKMTALQELPGLQHAKSVAIRLYPVSSTLGIA
                     EGIETALSCRQIFRCNVWSTMNSGFMEKFIAPPGVNHLIIFADNDEHGAGLAAAFKCG
                     HKNLMSRNDVEKVSIRWPDLPDFNDMLIQGCEAREHVLTRKFKAEAA"
     gene            complement(3528..5048)
                     /locus_tag="ACHKYO_RS16755"
                     /old_locus_tag="pgaptmp_003351"
     CDS             complement(3528..5048)
                     /locus_tag="ACHKYO_RS16755"
                     /old_locus_tag="pgaptmp_003351"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU4430140.1"
                     /translation="MLKIIPNFAQERGLNQLRHQWKQHRTYLMYAPTGSGKTGLAAFV
                     TAGMVERGMRVMFVCPYTILLNQTAERFTEYGLPWEEISFVWRDHPNYDPSRLIQIAS
                     ADTLIRREFPDNIDLLIIDEAHMKRRALLEVIRDRDIRVLGLSGTPFAAWMGKYYECL
                     IKPTTIRELIQRGDLSDYEFFAPSMPDLAGVKTSNTVFGRDYNEEQLASIMGSSDLVG
                     DIVRNWLENGEDLPTICFCVNVAHANFVTREFLQSGIGAEVMTADTPHDERQDIIRRF
                     EDGATKIIVNVGVLVAGFDSDVRCLIYARPTKSEIRWLQCIGRALRTAPGKKRALIFD
                     HSGTVHRLGFPEDIEYDELPGKNDGMKASAGGSEVKAEKLPRECSKCHFMKPAGVHMC
                     PKCGFRPLGGDDVATDRDRKLSRVNKGKREYSREEKQRWWSEIKGYQNYRNATGKPLS
                     DGWCAHTYKEKFGVWPKGFSNAPLQTSVEVHNFIRSKIIAYAKGRKKAMTGGQHAD"
     gene            complement(5042..5428)
                     /locus_tag="ACHKYO_RS16760"
                     /old_locus_tag="pgaptmp_003352"
     CDS             complement(5042..5428)
                     /locus_tag="ACHKYO_RS16760"
                     /old_locus_tag="pgaptmp_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430141.1"
                     /translation="MFIKHVVISLLGKKFYTGKDKVTFDYVLAAKLRDAGLAIERNYL
                     VDMGNGKRGFVDIMVVAPSGERCAIEVDRASPRARSILKLRRLKLYGIPGIVLLRCSR
                     NPDQYVCDEIDVIPATGKSRSKGASC"
     gene            complement(5650..5907)
                     /locus_tag="ACHKYO_RS16765"
                     /old_locus_tag="pgaptmp_003353"
     CDS             complement(5650..5907)
                     /locus_tag="ACHKYO_RS16765"
                     /old_locus_tag="pgaptmp_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430142.1"
                     /translation="MPDQYCDADRDWIQEQLLQLDPTTRVKIAAEYARVYQEEWDKEP
                     VSFRKGNRARRSANTRLRVYVQKYARASRGWMLSPVAIRKE"
     gene            complement(5954..6193)
                     /locus_tag="ACHKYO_RS16770"
                     /old_locus_tag="pgaptmp_003354"
     CDS             complement(5954..6193)
                     /locus_tag="ACHKYO_RS16770"
                     /old_locus_tag="pgaptmp_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4430143.1"
                     /translation="MTLDEYLRQSSIRQADFAAIVGVSQGFVSGVITGRYMPQGRKAI
                     EWAKATDWNVTPHELNPHDYPNMEDGIPLHVRQTA"
     gene            6299..7018
                     /locus_tag="ACHKYO_RS16775"
                     /old_locus_tag="pgaptmp_003355"
     CDS             6299..7018
                     /locus_tag="ACHKYO_RS16775"
                     /old_locus_tag="pgaptmp_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU4430144.1"
                     /translation="MTKKITITDKDIQNAERLRKIWDEKRKQLSLSQEKAADILGFKT
                     QGAVSQLLNAKIALNTENTLKFAALLQVPAEEINPDLSDLLRSIRTHTPGNRRNLFEK
                     YYEYPLLSCVQAGAFSMDDFSYTAKDAIKWISTTTKASDRSFWLEVKGHSMTAPQGGK
                     PSFPEGMLILVDPEREIEDGDFCVARMNGDEFTFKRFIRESGKAYLEPLNPRFDMIEC
                     NENCQFVGKVIKSQWNDETFD"
     gene            7140..7310
                     /locus_tag="ACHKYO_RS16780"
                     /old_locus_tag="pgaptmp_003356"
     CDS             7140..7310
                     /locus_tag="ACHKYO_RS16780"
                     /old_locus_tag="pgaptmp_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430145.1"
                     /translation="MNKDKSLTQQVEEILSKQKVSEQGKMIQSLKNMGLINKPVFTLA
                     YGPDITTCQMHQ"
     gene            complement(7425..7769)
                     /locus_tag="ACHKYO_RS16785"
                     /old_locus_tag="pgaptmp_003357"
     CDS             complement(7425..7769)
                     /locus_tag="ACHKYO_RS16785"
                     /old_locus_tag="pgaptmp_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGU4430146.1"
                     /translation="MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEASTLAELE
                     QEFKTSVELYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNK
                     ALEKEVNHHSGA"
     gene            complement(7766..8014)
                     /locus_tag="ACHKYO_RS16790"
                     /old_locus_tag="pgaptmp_003358"
     CDS             complement(7766..8014)
                     /locus_tag="ACHKYO_RS16790"
                     /old_locus_tag="pgaptmp_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGU4430147.1"
                     /translation="MGKADKLLEKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRF
                     FNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGVL"
     gene            8908..9681
                     /locus_tag="ACHKYO_RS16795"
                     /old_locus_tag="pgaptmp_003359"
     CDS             8908..9681
                     /locus_tag="ACHKYO_RS16795"
                     /old_locus_tag="pgaptmp_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4430148.1"
                     /translation="MGPTSVGLVPLIASSPNPVQSTASELRTSCGGSQSPIKEAAIMA
                     TIPALSQPEITITNGRVVTTSIAIAEFFDKRHDDVLKKIRLMDCSTEFNARNFADVTY
                     TDAKGEQRPMYQITKNGFVFLVIGFTGKKAAAFKEAYIAEFDRMEKQLWEQKQLALSS
                     PAMNINYPLSWFSEHHPYATMNYVDRKVISLDASVLFDMPSPTMRILNELHSKGYNVD
                     AAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
     gene            9937..10611
                     /locus_tag="ACHKYO_RS16800"
                     /old_locus_tag="pgaptmp_003360"
     CDS             9937..10611
                     /locus_tag="ACHKYO_RS16800"
                     /old_locus_tag="pgaptmp_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGU4430149.1"
                     /translation="MKTISVESLSIISFSNIPVVTTELLASLYGTEPDYIRKNFNRNS
                     GRFVIGKHYFLLENEELREFKHSMSLRPSVKIARNVRSLILWTERGAARHAKMLETDR
                     AWEVFEKLEDCYFSQKIPEQLQLPESTLSINYPLSWFSDNHPYATMNYVDRKVISLDA
                     SVLFDMPSPTMRILNELHSKGYNVDAAVAEFNAFKHLTEEMRRKLLDISRTSEKSSRF
                     GFNVNL"
     gene            10682..10894
                     /locus_tag="ACHKYO_RS16805"
                     /old_locus_tag="pgaptmp_003361"
     CDS             10682..10894
                     /locus_tag="ACHKYO_RS16805"
                     /old_locus_tag="pgaptmp_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HGU4430150.1"
                     /translation="MLKPHYGTALVSREDVKPGTAILYNGRYYMASANVNNALYAHSL
                     IEKIRIISDAIEVYLNNKGQPLISPA"
     gene            10906..11187
                     /locus_tag="ACHKYO_RS16810"
                     /old_locus_tag="pgaptmp_003362"
     CDS             10906..11187
                     /locus_tag="ACHKYO_RS16810"
                     /old_locus_tag="pgaptmp_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGU4430151.1"
                     /translation="MLNQEININVKSVITPAKTIMGEVFMDDKIIAYFVVLPDEAISV
                     IDTEGNVMFIAEHPEDIALQAAAYFFAKEQEEECNCPVCQLSRQINLMH"
     gene            11208..11489
                     /gene="gamL"
                     /locus_tag="ACHKYO_RS16815"
                     /old_locus_tag="pgaptmp_003363"
     CDS             11208..11489
                     /gene="gamL"
                     /locus_tag="ACHKYO_RS16815"
                     /old_locus_tag="pgaptmp_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor GamL"
                     /protein_id="HGU4430152.1"
                     /translation="MNAYLTCDRIEERRWANQQIQDEKDKWIDDRAQELISMFPDKPL
                     LMSSLFLPKEAQLALTGEKAEEAYNDYISAIAYARAEEEWERKFSPCPF"
     gene            11506..12456
                     /locus_tag="ACHKYO_RS16820"
                     /old_locus_tag="pgaptmp_003364"
     CDS             11506..12456
                     /locus_tag="ACHKYO_RS16820"
                     /old_locus_tag="pgaptmp_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HGU4430153.1"
                     /translation="MFDIVEFVKQQERFFCEALTEPTLTWAKESQFAIQQFQKNDFLA
                     KTAQANLPSARNAIINVAAIGITLNPSSKLAYLVPRGGAVCLDISYMGLLHLAQVTGA
                     IQWGQCKLVYEKDIYESNGIDCAPTHKYNPFVDRGARIGGYCVVKTSEGDYLTEEMSN
                     REIEVIRACSKAGGKGGSSPWDSFPDEMARKAIVKRASKYWPRRDRLDTAIDYLNTQA
                     GEGIILNADHIPERDVTPASDEIINEITQAMTEINKTWDDLLPICSKTFRRMIASHEY
                     LSQEEAVKTLDFVKKKAARNKATAEAKIHATTENNSEAVS"
     gene            12453..13142
                     /locus_tag="ACHKYO_RS16825"
                     /old_locus_tag="pgaptmp_003365"
     CDS             12453..13142
                     /locus_tag="ACHKYO_RS16825"
                     /old_locus_tag="pgaptmp_003365"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU4430154.1"
                     /translation="MTPEIILQRTGVDITSLDQGDDGWHKLRLGVITASEVHNVIAKP
                     RSGSKWPDTKMSYFHTLLAEVCTGVAPEVNAKSLAWGKQYEDDARALFEFIADVTVSE
                     TPIIFRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFDAIKPAYMAQVQF
                     SMWVTDKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIDKMDEALAEIG
                     FVFGEQWGVNN"
     gene            13142..13729
                     /locus_tag="ACHKYO_RS16830"
                     /old_locus_tag="pgaptmp_003366"
     CDS             13142..13729
                     /locus_tag="ACHKYO_RS16830"
                     /old_locus_tag="pgaptmp_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HGU4430155.1"
                     /translation="MDEVIFTYNEESALTAGQGGFITETGAHIINITEAELKQSEKGA
                     RFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGASIIHAMMGCTGIGQLTQHMVSVSK
                     YVAPEFHGKKIGLVLQKVLTTNRKTGADGYQMEIRIPFIAETGQTLKEKAEGKKPETV
                     ANMVSTLKDKDNRRKNVNPNHTDDPGYWQYGSDSF"
     gene            13804..14151
                     /locus_tag="ACHKYO_RS16835"
                     /old_locus_tag="pgaptmp_003367"
     CDS             13804..14151
                     /locus_tag="ACHKYO_RS16835"
                     /old_locus_tag="pgaptmp_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430156.1"
                     /translation="MNQHQTDVNVFINDLDGGVFVNKLGAVLSEVAFGVNSTNKKGKV
                     CVEFELSSLDENRVSVSHKLKFTRPTMRGSKSEEDTTNTPMFVNKGGELTLFQKDQGQ
                     LFDKQGQHDAVLR"
     gene            14215..15036
                     /locus_tag="ACHKYO_RS16840"
                     /old_locus_tag="pgaptmp_003368"
     CDS             14215..15036
                     /locus_tag="ACHKYO_RS16840"
                     /old_locus_tag="pgaptmp_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HGU4430157.1"
                     /translation="MSQLDSNAIKEIVKLTTTAFSGENLPLTECPVALLPDNVNIESL
                     ERFMTERFRFRGVMTTTSIDDFVEYSKGYADECSRCFINAETMKAVTVFNIGTLEQPG
                     HADNKALLELKATSPYRALRDVDGKRQLQKSLAEWLEDWADFLTAYDSDGNVLDIKQA
                     ISAIRRLTIDAKRSAEYEEQNFSGSRSVMESVEAKSKEIMPATFRFECIPYEGLGNRE
                     FTLRLSILTSEQPVLVLRIVRVEAAEEEIAKEFRDLLKERFEEEDILTFIGTFSV"
     gene            15113..15556
                     /locus_tag="ACHKYO_RS16845"
                     /old_locus_tag="pgaptmp_003369"
     CDS             15113..15556
                     /locus_tag="ACHKYO_RS16845"
                     /old_locus_tag="pgaptmp_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430158.1"
                     /translation="MQFKDLPADIQKIAADTLKAHLSVLDLTKEPKANLENISRNVRD
                     VFVGLYAYGNEKHEEHIQNGCINKFQQNIKLPVADKTEQKTEVLVFWNTVEAIARSTM
                     LEFNNINHSGIDTHQSSHREGFSILLTLLQEQGEYPQPTSNDTEQ"
     gene            15664..16542
                     /locus_tag="ACHKYO_RS16850"
                     /old_locus_tag="pgaptmp_003370"
     CDS             15664..16542
                     /locus_tag="ACHKYO_RS16850"
                     /old_locus_tag="pgaptmp_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HGU4430159.1"
                     /translation="MSTHHVVSFSGGRTSAYLVHLMEQRRKAGENVHYILMDTGCEHP
                     LTYRFIREVVKFWDIPLTVLQVDINPELGQPNGYTEWEPKDIQTRMPVLKPFMDMVKK
                     YGTPYIGGAFCTDRLKLTPFTKYCDDHFGQGNYITWLGIRADEPRRLKPKPGVRYLAE
                     LSDFDKSDVIRWWRKQPFDLQIPEHLGNCVFCIKKSTQKLGLACKDEPGLMRVFNELV
                     TGKHVRDGHRRTGKDVMYRGHLTLDGIARMYADSDYRNLYQAMVQAKRFDTGSCSESC
                     EIWGDQLELKFEEVVA"
     gene            16539..16742
                     /locus_tag="ACHKYO_RS16855"
                     /old_locus_tag="pgaptmp_003371"
     CDS             16539..16742
                     /locus_tag="ACHKYO_RS16855"
                     /old_locus_tag="pgaptmp_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430160.1"
                     /translation="MTIVKTHTGIVITKDGPQVKKLHQTERMWVVGKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPVEVKHV"
     gene            16735..16974
                     /locus_tag="ACHKYO_RS16860"
                     /old_locus_tag="pgaptmp_003372"
     CDS             16735..16974
                     /locus_tag="ACHKYO_RS16860"
                     /old_locus_tag="pgaptmp_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430161.1"
                     /translation="MFKQNEKSIAQIAEYIPRTCRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNCHTRESFFSCIAAQIHKGGNQ"
     gene            17097..17516
                     /locus_tag="ACHKYO_RS16865"
                     /old_locus_tag="pgaptmp_003373"
                     /pseudo
     CDS             17097..17516
                     /locus_tag="ACHKYO_RS16865"
                     /old_locus_tag="pgaptmp_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            17518..18237
                     /locus_tag="ACHKYO_RS16870"
                     /old_locus_tag="pgaptmp_003374"
     CDS             17518..18237
                     /locus_tag="ACHKYO_RS16870"
                     /old_locus_tag="pgaptmp_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4430162.1"
                     /translation="MTTMNREEQKQVLIDTANHVINRDNTSPYSENLRELARIALASL
                     AAVSDERAAYELFMEKRFGESVDRRRAKNGDREYMAWDMALGWIIWCHRAAMLHCAEP
                     ASKHEELPLDYLQGQKDGLEWAAQLAEANHPQTGDWLYDDPLDLAKAIRKGPDIPEFA
                     GPHPVTPDGWISCSDRMPEDTKMLLAFSQGEIVAAYWNWVVNPIDYKKYRAFTYLSGY
                     ILDDVTHWMPLPEPPQEVNRG"
     gene            18230..18514
                     /locus_tag="ACHKYO_RS16875"
                     /old_locus_tag="pgaptmp_003375"
     CDS             18230..18514
                     /locus_tag="ACHKYO_RS16875"
                     /old_locus_tag="pgaptmp_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGU4430163.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYEGYEIKTITHTHFGDKPVKVFAIKVNIGSD"
     gene            18563..19303
                     /locus_tag="ACHKYO_RS16880"
                     /old_locus_tag="pgaptmp_003376"
     CDS             18563..19303
                     /locus_tag="ACHKYO_RS16880"
                     /old_locus_tag="pgaptmp_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430164.1"
                     /translation="MINNIANQSSFPAATIDSQMLLKMVNEARKLCSEKPVRNNDFIA
                     RVKDELEGETYEIFVGQKNGAEIEVITMTFKQALRVAARESKAVRRSLVDKLESMQQQ
                     LQQKTTTKKSPDGLEEFRKARALKMTVDTMKDLFDFLPNLAPEAKQVVAASLVNPVVG
                     ANVIPLPMINEHYYSASEVGAKLKISANKVGRIANTYMLKTEQYGKWFIDKSPYSDKQ
                     VESFRYNNRGVRKIEEILDAENKAEFGT"
     gene            complement(19346..19651)
                     /locus_tag="ACHKYO_RS16885"
                     /old_locus_tag="pgaptmp_003377"
     CDS             complement(19346..19651)
                     /locus_tag="ACHKYO_RS16885"
                     /old_locus_tag="pgaptmp_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4430165.1"
                     /translation="MAMFNPPHPGEIIADILEDQNIGIRELARALDVAPSTVQRLVSG
                     NATISPEMAVRLAAVLGGTPSSWIRLQTAWSLEKAEREVDVSHLSTKYRPAEISPHA"
     gene            complement(19670..19978)
                     /locus_tag="ACHKYO_RS16890"
                     /old_locus_tag="pgaptmp_003378"
     CDS             complement(19670..19978)
                     /locus_tag="ACHKYO_RS16890"
                     /old_locus_tag="pgaptmp_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4430166.1"
                     /translation="MTGKRNPPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(19968..20297)
                     /locus_tag="ACHKYO_RS16895"
                     /old_locus_tag="pgaptmp_003379"
     CDS             complement(19968..20297)
                     /locus_tag="ACHKYO_RS16895"
                     /old_locus_tag="pgaptmp_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4430167.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLIERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGCDGIARVFYCTLAGKRIIMLHSFIKKTQKTPPAELKKAETRM
                     KEVKHDW"
BASE COUNT         5634 a         4971 c         4654 g         5202 t
ORIGIN      
        1 taaaatatac aatatttaga aaactaatag tgccatatgc gatttttaag attttgttat
       61 taattgcggt cgcaccttcc tttctgtgta ctttccgtat aacttacagg attccggata
      121 caaaaaaccc gcgcatcggc gggttcggct gcgtggcaat gtaaccactc ttatcatgat
      181 atgcagattt ttacgtacgt aaaatacttt ttagcacctc gttcaatcgg gagtggaatc
      241 cggggttccg cgacgcttca caccacctct ggcggagatt ttcggggctt ttttttgcca
      301 attagcgatg aattcagtct gcgttgtttc gtgcttctgc atgaacggca ccagctttgc
      361 ggccttttct tattcccgca aattctgaat ccgaattttt tctcaatttc ctcattagat
      421 atagcgactt tcccgcagag ggggcaagta tgctttttat cacaaccact tttcaataac
      481 caaattaacc ggcggattaa tggcttgata tcatagatgt ttcgcaaatc atcgtcatga
      541 tcgatttgcg cgtaacttat cttctgtaac atatctttct cctccagaca cactaggact
      601 ccttgtatgg cgtcccggga tgtacgcatt ctggtggcaa aaagagatat tgatggtaat
      661 gtttgcgaat ctacgtcctg gtgaaatgaa acaaaattag ccagcagaag taattctgaa
      721 tagtttaact ttaacctgtt ctggtatttc aaaagctctt tgaggataac gaaccagtca
      781 atgcttaaaa tatcatcatt ccacagagac agatcattgt cttccgcctc cagaaaatct
      841 tcaatgtcca ttttgccccc tgttcattgt tgtatatatg ttattaacag gtagataact
      901 cttacgtccc tgtactataa agtaaacagc ataacgctca caaattgaca cacttcacat
      961 agtgaacggc aaacgcctcc tgtcagaggc gttgcacata tcggtccatt tccagtctga
     1021 caccaagtaa cattaacatc ccttcaacca caccctccgc tttctgtaat tttttaccga
     1081 tagttccatc agagcatcca tgcttacggg ccagcgccat gaacgtctcc cccaacacgt
     1141 aataatcaac cagcaagtca tgcagatcgc tgttgttcct gttaaggcga gccatacacc
     1201 cgcatataat catcgcgtca tcgtcacaac actgtggacg tgattttact ttttcgggga
     1261 tcagtccctt aaatccggca gcaatgggcg accatgtaac atcctcatag ttatttgccg
     1321 cccatgcacc ccagcgctca agaacctgcc ggatatcacg catcagtatc tttaccccat
     1381 ccgcgatgaa ccataagaac accgttgacg atagcgtgtc tttttccttc tttatcgcca
     1441 gtatattttc tgaccgtgtt gcgactacag ttcagtattc tggcgacttc tgtctgattt
     1501 ccatatgcct caaggagcat gtcaggaatg gtttttactg tgaacgtcat gcggcctcac
     1561 ttctgctgtt tcgcaggtct ttaagtttct gctgatactt cgccttgatc gccctgcatt
     1621 cttcgacagt ccagcgatgg cggttatggt tcgattcgat ttcgtctact gcttcctgcc
     1681 cgatgcggtt aatcagttcg acgcgatacg gaacgagatt tccgcttttg tactggttac
     1741 acaccacgca ttgcttgtgg atattgcgtt catcaaatct gagttgggga gccgcaactg
     1801 ttgtacggta atgacctgca tcccactgag cagaatcgaa tgtcccgcat gaaacgcacg
     1861 gcaaattacg gtctcgttcc ctgatataag cattcacggc ctgctgtgcc tgcttaatcc
     1921 agtaactgcg gggctgtaag tcccttcttc gcgctttaag tttgtctgcc tgttcttttg
     1981 cagaggctat ggccttttct tttctgacca gttcaagtgc gcactgaccg caacaaactt
     2041 tctgatacga gcgaaacggc acaaacgggg ttccgcacac tttgcatttt ttaggtttac
     2101 gttgctgcat catcacctca gaaaaacgac agcaggcggt tattcagttc agggctgttt
     2161 gagcgcccga acacatgttt taacgcggca ttaatcattg cgttgtagca acgttcaaac
     2221 tcatcctgat ccatattcgc gtaactcagg ctttttgccc gatatctcac ctcgcctctt
     2281 atggttgtga ccacatcata gaacccggca agaattgtca gatttttacg aaactcatca
     2341 aactgcgtcg cttcatcaga gaattcgtat ccagcatgtt cagcgcacca gtactgaaag
     2401 cagaaattaa gaaaagcgaa catcttccgg tgaaaagatg ggttacgggt aagttttgct
     2461 tccagtgtat acagctcacc gtttttaaat tttgccagtc ggggtaaatc gcgctcatac
     2521 gccggtacaa atacgccatt agccgccttg atcatctcta tttccatcag gcagcctccg
     2581 ctttgaattt gcgcgtcaac acatgttcac gggcttcaca cccctgaatg agcatgtcgt
     2641 taaaatccgg caagtcaggc cagcgaatgc tgaccttctc aacgtcatta cgactcatga
     2701 gattcttatg cccgcattta aaggcagccg ccagacctgc gccgtgctca tcattatcag
     2761 cgaagataat caggtgatta acgcctggtg gcgcaataaa cttctccata aaaccggagt
     2821 tcattgttga ccacacattg cagcggaaga tttgacggca tgaaagcgcc gtttcgatac
     2881 cctcggctat ccccagagtg gacgacaccg gatacaggcg tattgccacc gatttggcat
     2941 gctgcaaacc aggcaactcc tgtagcgcgg tcattttttt agccgcctct acattcgcct
     3001 tgcgatcacc atccagcaac gtacgatgca gatagcaaag agaacctttg tcatctgttg
     3061 caatggcgta aattgcctga tattcgcgcc cgctggcgat ctgacggtca cagaaacgca
     3121 cggattcggt cggtaactgg agtattcccc tcccctgcag gtacgcctca cctgttgtgc
     3181 cccgcagaca tgaaagtctg gagaacttat ccatgaccag cttccgcttt ctgcttatct
     3241 ctgttacagg ctggctgact ttctccctct tccaggtatt tccgatcagc cgatcgattt
     3301 catcacacaa cgtcacccag ggcttacccg tggcaagttg caacaacttc atcccgtccc
     3361 cgtgaccaca gacgcaaatc cacgaaccgg agccatcttt atcatcacag cgaaatttac
     3421 ctctggcacc acatatcggg catgggccgt tgtaatgttt cacaccagta acaggaggca
     3481 tgccgtaata ttcaaaaatt cgcccccatt gcccgacagc cgccttctta gtctgcatgc
     3541 tggcctccgg tcatggcttt cttgcgccct ttggcatagg caatgatctt tgacctgatg
     3601 aaattgtgca cttcaaccga ggtttgcagc ggcgcgttac tgaagccttt aggccacacc
     3661 ccgaacttct ctttgtaggt atgagcgcac catccgtcac tcagtggctt acctgtcgcg
     3721 ttgcggtaat tctgataccc cttgatctca ctccaccacc gctgtttttc ctcgcggctg
     3781 tattcgcgtt tccctttatt tacgcgtgag agttttcgat cgcggtctgt tgccacatca
     3841 tcgccaccaa gaggacgaaa cccacatttg ggacacatgt gtacaccagc aggcttcatg
     3901 aagtggcatt tggagcattc tctgggaagt ttctcagcct taacctcact cccgccagca
     3961 gacgctttca ttccgtcatt ttttcccgga agttcgtcat actcgatatc ctcagggaat
     4021 cccagccgat gaaccgtgcc agaatggtcg aaaatcaacg cacgtttttt acctggggct
     4081 gtacgtaacg ctctacctat gcactgcaac caacggattt ctgattttgt cggacgggca
     4141 tagatcaggc agcgaacatc actgtcaaac cccgctacca gtacgccgac gttgacaatt
     4201 attttggtcg caccatcttc aaaacggcga atgatgtcct gccgttcatc atggggggta
     4261 tctgccgtca tcacctctgc gccaatgcca gactgtaaaa actcacgggt aacaaaattc
     4321 gcatgcgcaa cattcacgca gaagcagatc gtcggcaaat cctcgccatt ctcaagccag
     4381 ttgcgaacga tatcgccaac cagatccgaa ctccccatga tggatgcgag ctgctcctcg
     4441 ttgtaatccc gtccaaatac ggtattactg gtttttacgc cagccagatc aggcattgag
     4501 ggggcaaaaa actcgtaatc gctcagatca ccacgctgaa taagttcacg aatcgttgta
     4561 ggcttgatca ggcattcgta gtacttcccc atccaggctg cgaacggggt accggaaagt
     4621 ccaagaaccc tgatgtctct gtcccggata acctcaagta acgcacgacg cttcatgtgt
     4681 gcttcatcga tgatcagcag atcgatgttg tcaggaaact cacgacggat cagagtgtcg
     4741 gcactggcaa tctgaatcag tcgtgacggg tcatagttag ggtgatcacg ccatacaaaa
     4801 ctgatttctt cccacggcaa accgtattcg gtgaaacgtt ccgctgtctg gttcagcagg
     4861 atggtgtacg gacagacaaa catcacccgc ataccacgtt caaccattcc ggcggtgacg
     4921 aatgctgcaa gaccagtttt gccagagcca gtgggtgcgt acatcaggta cgtgcgatgc
     4981 tgtttccact ggtgccgcag ctggtttaaa ccgcgctcct gagcaaagtt tgggattatt
     5041 ttcagcatga cgccccctta ctacgtgatt tacccgttgc cgggatcacg tcgatctcat
     5101 cgcaaacgta ttgatccgga ttacgtgaac agcgcagaag cacgatacca ggaatgccgt
     5161 atagcttgag tctgcgcagt ttcagtattg aacgtgcccg tggtgacgct ctgtccacct
     5221 cgatcgcgca ccgttcacca gatggggcaa cgaccatgat atcgacgaat ccgcgcttac
     5281 cgttgcccat atcaaccaga taattacgtt cgatcgccag tccggcatca cgcaatttgg
     5341 cagccagcac ataatcaaac gtgaccttgt cttttccggt gtagaatttc tttccgagca
     5401 ggctgataac cacatgtttg atgaacatgg tactatttcc actgtgatag ttagcatgat
     5461 aataatttag ccagaaacta tcgcgcccta tacagtgatc tacctaacct atggagctgc
     5521 ctttctctgg tagtgccctt acgggcacgg atgttacaga tctgccccct tacccccttc
     5581 ttagtaaaat ttcaagccaa ataatttcac acgaaatcat ttcacaaaaa acaatcacct
     5641 tgatgaggtc tattccttcc gtatcgctac tggcgaaagc atccagccac ggctggctct
     5701 cgcgtacttc tggacataca cccgtaaccg ggtgttggcg ctccgtcttg ccctgttgcc
     5761 cttcctgaac gatacaggct ccttgtccca ctcttcctgg tacactctcg cgtactctgc
     5821 tgcaattttt accctggtgg tcgggtctag ctgtaacaat tgctcctgta tccagtcccg
     5881 atcagcatca cagtactgat cgggcataac cgttttgatt tgcctgttca ctcacacctc
     5941 cgatggaggg ttatcaggct gtttgcctta catgaagcgg aataccatct tccatgtttg
     6001 ggtagtcatg cgggtttagt tcatggggag ttacattcca gtctgtagct tttgcccatt
     6061 caatggcttt ccgcccctga ggcatgtatc tccctgtaat aaccccactg acaaatccct
     6121 gagaaactcc aacaatcgcc gcaaaatcgg cttgtctgat agagctttgt ctcaagtact
     6181 cgtcaagagt catagagatc ctcccatttc aatttttcga tattagctta cctgatttaa
     6241 atgtcaatag gctagctatt tgataaatat ttgtgttgct aataaaatcg gaggcactat
     6301 gacgaagaaa attacgatta ctgacaaaga catccagaat gctgaacgac tgcgcaaaat
     6361 ctgggatgaa aaacggaaac agctatccct cagccaggaa aaagccgcag atatcctcgg
     6421 cttcaaaacg cagggggctg ttagtcagtt attgaacgca aaaattgcgt taaatactga
     6481 aaataccctt aagtttgcgg ctttattaca ggttcctgcg gaagaaatta atccagatct
     6541 gagtgatttg ttacgcagta ttcgtacgca cacaccggga aacaggcgta acctgttcga
     6601 gaaatattac gaatatccat tactctcctg cgtgcaggct ggcgcattct caatggatga
     6661 tttttcgtac accgcaaagg atgcgattaa gtggatctcc accaccacaa aagccagtga
     6721 caggtcgttc tggctggagg tcaaggggca ttcaatgacc gcgccacagg gaggtaaacc
     6781 cagcttccct gaaggtatgc tgatacttgt tgaccctgaa cgggaaatcg aggatggcga
     6841 tttttgcgtg gcccgaatga acggcgatga attcaccttc aaacggttca ttcgtgagag
     6901 cggtaaagcg tacctggagc cactcaaccc acgattcgac atgattgagt gtaacgaaaa
     6961 ctgccagttt gtcggaaagg tcataaagtc gcagtggaat gatgagactt ttgattgaac
     7021 tcacaggatg atatcaataa ccttgcaggg aggctatttt atttcctctt tcagcgaaaa
     7081 atctccgaga accctcgccc aatgcgggcg gggagcagtc gcaatcaagt gagccataaa
     7141 tgaacaaaga caaatccttg acacaacaag tggaagaaat acttagcaag caaaaagtta
     7201 gcgagcaagg aaaaatgatt caatcattaa agaacatggg cttgatcaac aagcctgttt
     7261 ttactttggc ttatggacca gatattacaa cctgccaaat gcaccaataa caaaacccgg
     7321 ccttggtgcc gggttttgtt tgcctcctgc tcgccccacc atttaccagt gcgccgtaaa
     7381 taccccattc ttcggcggtg aggatgtcaa agacgaaggg aacattaagc ccctgagtga
     7441 tggttgactt ctttttccag cgccttatta acaaaggcgt tcagtgataa atcttcttcc
     7501 atcgccattt cagcgacccg acgatgcagt tctggatcaa gtctgacgtt aaacacacct
     7561 ttaaacgggg tatcaggctc ctttccatcc tccacacaag actgtaaata cagctcaacc
     7621 gatgtcttaa actcctgttc cagttcagct aatgtagaag cctcataagt taccaggtct
     7681 cgaataaacg ccagttttcc gtacaggata ttattttcaa aatctggttc tactgtacct
     7741 aaatatcctt tatattttag atgcttcata ataccccagc ctctttcaga ttctgtttaa
     7801 tcgctttcag cgttccacct ttaatataac tttctggatg tgggcgatgc attaatatgg
     7861 tgtggttgat ttcagcattg aaaaaccgca ctcttgagcc ctgcatttcc tttttgacat
     7921 atcccagaga ggaaaacaaa acgaccagct catcccattc aaacgttttt ttactgttta
     7981 aaaacttttc cagtagctta tctgctttcc ccatacccac ataatctcat cttcgctaca
     8041 ttgcaactaa ttatagttac aggcagtgtt tttgtcaaaa gccaccaagc ccgctgggtt
     8101 ctctttgact gttcgctgct tttcctggtg ttatccgggg gcttttccct cacgccaaat
     8161 ctcacacaga gtaaccggcc tcagcgtcgg tttttctttg cgcatcctcc ataactcacc
     8221 acctgcatta tcctcgctta tattttttac ataaataaat taatctaaaa atcaatcaca
     8281 tagattaaaa ttaaatcaaa ttattagtct tactattgac tatgataagt tgagcttatt
     8341 atattttacc caagccagaa acatcgcgcc cttacacagg gctacataaa tcagtcgtac
     8401 ggcgcgactt aacccgccgc aaaatgctct ttaacaatct ggagctttac agcgtcaatg
     8461 acctgtttag acccctacac gtaaacgtgc tgtatcatcg ggtgcgatcc ggtcgatgag
     8521 agagtatccc cgcgcgagag cgagaacggc gtgagaacgg gcaacactgg caggaagttg
     8581 gcgctgacca atacagggaa tgttttgggg tgtggtgaag ggttcatgga cgggaatatg
     8641 tcgcacgtaa agcggcgagg cccgagggac tattgctgaa gataagtagg ccgaatcggg
     8701 tcgaaatggg tctcccacct gccacatcgc caaagcattt ccgagaaagg acggtattga
     8761 ctgcgtgtga tgtcggggct atatttggcg aacaccttat aaaacgggtg tcgggattag
     8821 ggccccgctg acaatcgagg cgatacagac gcgccagcgt ctttttttgt atcgacatac
     8881 acgcacatct tatcaatggt gggctgtatg gggccgactt cggtcgggct ggttcccttg
     8941 attgccagta gtcctaaccc tgtacagtcc accgccagcg agcttaggac ctcctgcggt
     9001 ggttctcaat caccaatcaa ggaggctgcc attatggcta ctatccctgc cctttctcaa
     9061 cctgaaatca ccattaccaa tggtcgcgtt gtcacaacgt ctattgctat tgctgaattt
     9121 tttgacaagc ggcacgacga cgttttaaaa aaaatccgat tgatggattg ttctacggaa
     9181 tttaacgccc gcaattttgc ggacgttacc tacaccgacg ccaaaggcga acaacgccca
     9241 atgtaccaaa tcaccaaaaa cggattcgtc ttcctggtga taggattcac tggtaaaaaa
     9301 gccgctgctt tcaaagaagc ctacattgct gaattcgatc gcatggaaaa acaactatgg
     9361 gaacagaaac aactggccct gtcttctcct gcgatgaaca tcaactaccc tctctcgtgg
     9421 ttctcagagc atcaccccta tgccacgatg aactatgttg atcgcaaggt tatcagcctt
     9481 gatgcttccg tgctcttcga tatgccaagc ccaactatgc gcatcctcaa cgagctacac
     9541 agcaagggct ataacgtcga cgcagctgtc gctgaattta acgccttcaa acatctgacg
     9601 gaagaaatgc gccgcacgct acaggatatt tcaagactgt cagatcgaaa ttcccgaaaa
     9661 ggcttctcgt taagtcttta aagaacagat cgtttgacaa tgctgcatta acggggatat
     9721 attctgcctc atggtgctca aaacacctta gcaagtagcg gttaccgcgc ccgacagaca
     9781 tgcggttttt ttgtgtccag tcttcttggt ttatgaccgg gcgtgcggct aatacaatac
     9841 cagcaatggg aatatgcccg ccgactactt gcggttttga gcgcccggtc accctctcaa
     9901 aaggggtaaa tcaaaatatc aagtaggacg caaagcatga aaaccatctc cgtggagtcc
     9961 ctctccataa tttcatttag taatatacct gtggtgacta cagagcttct tgccagctta
    10021 tatggcacag aaccagatta catccgaaaa aatttcaatc ggaattctgg acgatttgtt
    10081 atcggtaagc actacttttt acttgagaat gaggagttgc gggaatttaa gcacagcatg
    10141 tctttaagac cttctgtgaa aatcgcccgt aacgttcgct ccctcatcct ctggacagaa
    10201 cgcggcgcag cccgtcacgc aaaaatgctc gaaaccgatc gggcgtggga agtgttcgaa
    10261 aaactggaag actgctattt cagtcaaaaa atcccagaac aacttcagct tccagaatca
    10321 acgctatcta tcaactaccc tctctcgtgg ttctcagata accatcccta cgccacgatg
    10381 aactatgttg atcgcaaggt tatcagcctt gatgcttccg tgctcttcga tatgccaagc
    10441 ccaactatgc gcatcctcaa tgagctacac agcaaaggct ataacgttga cgccgctgtc
    10501 gctgaattta acgccttcaa gcatctgacg gaagaaatgc gccgtaaatt gctggacatt
    10561 tcccgcacat cggaaaagtc ttcccgcttt ggtttcaacg taaatcttta attaacccca
    10621 tccccgacca catatcgggg attaaattaa atatctggat taattaaccg gaggatttgt
    10681 catgctcaaa cctcactacg gaaccgcatt agtttctcgc gaggacgtta aacccggcac
    10741 tgcaattctt tacaacgggc gttattacat ggcgtcagct aacgttaata atgcacttta
    10801 cgcacattca ctgattgaaa aaattcgcat tatctcagat gcaatagaag tttacctgaa
    10861 caataaaggc caaccgttaa tctcaccagc ctgaaaggaa atatcatgct caatcaagaa
    10921 attaatatca atgtaaaaag cgtcataacg ccagcgaaaa cgataatggg agaagttttt
    10981 atggatgata aaatcatcgc ctattttgtc gtcctgcctg atgaggctat ttctgttatt
    11041 gatacggaag gcaatgttat gtttatcgcg gaacatccag aagacatcgc attacaggcc
    11101 gctgcatatt tcttcgctaa ggagcaagag gaagaatgta actgccctgt ctgtcagctt
    11161 tcccgacaaa ttaatttaat gcattaaccg gaatcaggag ttccgccatg aacgcatacc
    11221 tcacctgcga ccgcatagag gagcgtcgct gggctaatca gcaaattcag gatgaaaagg
    11281 ataaatggat tgatgatcgg gcgcaggagc ttatcagcat gttccccgat aaaccattgc
    11341 ttatgagcag ccttttttta cccaaagaag cccaactggc actcactggc gaaaaagctg
    11401 aagaggcgta caacgattat atctcagcga tcgcctatgc tcgggcggaa gaagaatggg
    11461 agagaaaatt ctccccctgt cctttctgat tttcaggacc aaaaaatgtt cgatatcgtt
    11521 gaatttgtta agcagcagga gcgttttttc tgcgaggcat taactgaacc gacgctgaca
    11581 tgggcgaagg aaagtcagtt tgcaattcag caattccaga aaaatgactt tctggctaaa
    11641 acagcacagg caaatctgcc cagcgcacgg aacgctatca tcaatgttgc cgccatcggc
    11701 ataaccctga acccgtccag caagctggcg tatctggtcc cacgaggcgg tgctgtatgc
    11761 ctggatatca gttatatggg acttctgcat ctggcacagg tcacaggagc cattcagtgg
    11821 gggcaatgca aacttgttta cgagaaagac atttacgagt ccaacggtat tgactgcgcc
    11881 cccacgcaca aatacaaccc attcgtagac aggggcgcac gcattggcgg ttattgtgtc
    11941 gtaaaaacat ccgaaggcga ctatctgacc gaagagatga gcaacaggga aatcgaagtc
    12001 atcagggcgt gcagcaaggc cggagggaaa ggaggaagta gcccgtggga tagtttcccc
    12061 gatgaaatgg ccagaaaagc cattgttaag cgcgccagca aatactggcc ccgtcgcgat
    12121 cgcctggata cagctatcga ctacctgaac actcaggccg gtgaaggtat catcctgaat
    12181 gctgatcaca tccctgagcg tgacgtcact cccgcatcag atgagattat caatgagatc
    12241 actcaggcaa tgaccgaaat taacaagaca tgggatgatc tgcttcccat atgttccaaa
    12301 acattccgtc gcatgattgc atcacatgaa tatctcagtc aggaagaagc tgtcaaaacg
    12361 cttgattttg tcaaaaagaa agctgccaga aacaaggcca cggcggaagc gaaaattcac
    12421 gccaccacgg aaaataacag cgaggccgtg tcatgacacc agaaattatt ctccagcgaa
    12481 ctggcgtgga tattaccagc ctcgaccagg gcgatgatgg atggcacaag ctgagactcg
    12541 gtgttatcac tgcttcagaa gttcacaatg tgatagcaaa gccacgttcc ggcagcaaat
    12601 ggcctgatac aaaaatgtca tacttccaca ccctgctggc tgaagtttgt actggcgtgg
    12661 caccggaagt taacgctaag tcgctcgcat ggggaaagca atatgaagat gatgcccgtg
    12721 ccctctttga atttatcgcg gatgttaccg tctcggaaac acccataatt tttcgtgacg
    12781 aaagcatgcg caccgcctgc tctcccgatg gtttatgcag cgatggtaac ggtcttgagc
    12841 ttaaatgccc ttttacttcc cgcgacttca tgaaattccg gcttggcggc tttgacgcta
    12901 tcaagcctgc ttacatggcc caggtgcaat tcagcatgtg ggttacagac aaggacgcct
    12961 ggtacttcgc caactacgac ccacgcatga agcgtgaagg cctgcattat gtcgtggtcg
    13021 aacgggatga aaagtacatg gcgagttttg atgagatggt gccggagttt atcgacaaaa
    13081 tggacgaagc actggcggaa attggttttg tatttggaga acaatggggg gttaataact
    13141 aatggatgaa gtgattttta cttataacga agaatcagca ctgaccgccg gacaaggtgg
    13201 ttttattacc gaaacgggtg cgcatatcat taacatcacc gaagcagaac tcaagcaatc
    13261 agaaaaaggt gcccgattca ttgagttttc tggagaatcc gacgacggac ggaaaatcca
    13321 gtatctcagt gtttgtgttc agaagaatga cggcactgaa aataaatttg gggcgagcat
    13381 tattcacgcc atgatgggat gcacaggaat tgggcagtta acgcagcata tggtttccgt
    13441 cagtaaatat gttgcacctg agtttcacgg aaagaaaatc ggactcgtcc tccagaaagt
    13501 attaaccaca aacagaaaga ctggcgcaga cggttaccag atggaaatcc gcattccatt
    13561 cattgcagaa acagggcaga cactgaaaga aaaagctgaa ggaaagaaac cagaaacggt
    13621 agcaaatatg gtttccactc tcaaagacaa agacaatcgc agaaaaaacg taaacccgaa
    13681 tcacacggac gacccaggtt actggcagta cggcagcgat agtttttaat tcacaaacaa
    13741 aaccaggctt taaatgcagt gaacaactga agccttaaca cacctctaca cggagatatt
    13801 aatatgaacc agcatcaaac tgatgttaat gttttcatta acgacctcga cggcggggta
    13861 tttgttaaca aacttggtgc ggtattaagt gaagttgcct ttggcgtaaa cagcacaaac
    13921 aaaaaaggaa aggtatgtgt tgaattcgaa ttatcttcac ttgatgaaaa tcgcgtatca
    13981 gtttcccata aactaaaatt cacacgcccg acaatgcgtg gtagtaaatc agaagaagac
    14041 acaactaaca ccccgatgtt tgtaaataaa ggtggtgagc ttactttgtt ccagaaagac
    14101 cagggacagc ttttcgataa acagggtcaa catgacgctg ttttacgctg aataatcccc
    14161 gccttaaaac gctcgcgcat tatccctaaa tccataaatt aaaggtaaat atacatgtct
    14221 cagttagata gcaatgccat taaagaaatt gtaaaactca ccactactgc tttttctggt
    14281 gaaaatttgc cgcttacgga atgccctgtt gcgttattgc cggacaatgt aaatatcgaa
    14341 agtctggaac gattcatgac agaacgtttt cgtttccgtg gagtcatgac cacaaccagt
    14401 attgatgact ttgttgaata cagcaaaggg tatgccgatg agtgctcccg ctgcttcatt
    14461 aacgcggaaa cgatgaaagc agtcactgtg ttcaatatcg gcactctgga gcaacctgga
    14521 cacgctgaca acaaggcact gctggaactg aaagccacat caccatatcg cgcattacgt
    14581 gacgtggacg gcaaaagaca actccagaaa tctctcgctg aatggctgga agactgggcc
    14641 gacttcctca cagcctatga cagcgacggt aatgtgctgg atatcaaaca ggctatctct
    14701 gctatccgtc gtcttaccat cgacgcaaaa cgcagtgctg aatatgaaga gcaaaacttc
    14761 agcggtagcc gctcagtcat ggagtccgtg gaggctaaaa gcaaagaaat catgcctgcc
    14821 actttccgct ttgaatgcat cccatacgag ggcctgggca acagggaatt cacgctgcga
    14881 ttaagcattc tgacaagcga acagcctgtt ctggtgctac gtatcgtgcg tgttgaagct
    14941 gcggaagaag aaattgccaa agagttccgt gacctgctga aagagcgttt cgaagaagaa
    15001 gatatcttaa ccttcatcgg cacgttctca gtgtgatgaa atctgtgaca gggacgtcac
    15061 gacatatcgc tcatatttca cgagaaaata cacagttcct ggagggtata ctatgcaatt
    15121 taaagattta ccagcggata ttcagaaaat agctgcggat acgcttaaag cccatttatc
    15181 ggtgcttgat ttaacaaagg aaccaaaggc gaatctggaa aatatatccc gtaacgtgcg
    15241 agatgttttt gtcgggctgt atgcttatgg caatgaaaag cacgaggaac atattcaaaa
    15301 tggttgcatt aataaatttc aacaaaatat caaacttcct gttgctgata aaacagagca
    15361 gaaaacagag gtattagtat tctggaatac cgttgaggct atcgccagga gcacgatgtt
    15421 ggagtttaac aacatcaatc attcagggat cgacactcat caatcatcac atcgtgaggg
    15481 gttttccatt cttctaactc tgttacaaga gcaaggcgaa tatcctcagc cgacatcaaa
    15541 cgacactgag caatagtcca ttctcgtacg ctgcaacgtg aggtcgtacc ccggacacgg
    15601 gtaaacatcc ggcaattcca gcttaccacc tatttaaccc cctccccgta acgagacgaa
    15661 gaaatgagca ctcatcatgt tgtgagtttt tcaggcggtc ggacatcggc gtacctcgtc
    15721 caccttatgg agcaacgcag aaaagctggt gaaaacgttc attacatact catggatacg
    15781 ggatgtgaac acccgctgac gtaccgattt attcgggagg ttgtgaagtt ctgggatatt
    15841 ccgctaaccg tgttgcaggt cgatataaat ccagagcttg ggcagccaaa tggttatacg
    15901 gaatgggaac caaaggatat tcagacgcga atgccggtgc ttaaaccgtt tatggacatg
    15961 gtaaaaaaat atggcacgcc atacatcggc ggcgcgttct gcactgacag attaaaactc
    16021 acccccttca caaaatactg cgatgaccat ttcggacaag ggaactacat cacgtggctg
    16081 ggtattcgtg cagacgaacc ccgtaggctg aaaccgaaac cgggcgtccg gtatcttgcc
    16141 gaactgtcag attttgataa gtcggatgtt atccggtggt ggcgaaaaca accttttgat
    16201 ttgcaaatcc cggagcacct cgggaactgt gttttctgca tcaaaaagtc aacgcaaaag
    16261 ctggggcttg catgtaaaga cgaacctggt ctgatgcggg tttttaatga actggttaca
    16321 ggcaaacacg tcagggatgg tcatcgcaga acaggtaaag acgttatgta ccgtggccac
    16381 ctgacgcttg acgggattgc cagaatgtat gccgacagcg actacagaaa tttgtatcag
    16441 gcgatggtgc aggccaagcg attcgatacc ggctcgtgtt cagagtcatg tgaaatctgg
    16501 ggtgaccaat tggagttgaa attcgaagag gtagtggcat gacaatcgta aaaacccata
    16561 ccggcattgt gatcaccaaa gacggcccgc aggtaaaaaa acttcaccag acagagcgga
    16621 tgtgggtcgt cggcaaaaac gagttttacc acaaagaaac cggacgccgc cactttgcag
    16681 aaaatacgcg ccgcagactg ctgatcgaca caatcaagcc tgtcgaggtg aagcatgttt
    16741 aaacagaacg aaaaatctat cgctcaaatt gctgagtata tcccgcgtac gtgccggggt
    16801 atgcagttgc aggaagccaa agcgcgcctg gagaaaaaaa ttgcgctcta tatcgatgac
    16861 ggctgtgatg ctgccgttct taacgcggct ttcgcaccag ctcttaactg tcatacgcgg
    16921 gaatcttttt tttcgtgcat cgcagcgcag atccacaaag gaggcaacca gtgagcgaaa
    16981 ttaattacca ggcactgcgt gaggcggcag tagcaattga aacagtagca acgcctcaaa
    17041 aattgctggc atttcgtatg aaagccacac ctcaggttgt gctggcgcta ctggatgagt
    17101 tggaagcagc aaaatcaaaa ctcaacgagc agcgtgagta ttacgaggga gtaatcgcgg
    17161 atggaagtaa gcgcatagcc gaacaggaga aaagcgaaga gcaactcatt aacgagcgtg
    17221 accatgctga gtctgcttta gctgatatgt attttgcagc aaccggggat aggcctgagt
    17281 ggagtaactg gttcggtttt tcagatgcag tagatgccgt ggtggacaga attgctgatt
    17341 tagaagctaa acagccatcg ccagtagtac ctgaaggact gattaaagcg gtgcgttttt
    17401 atgagcaggt aaaaaatgag aatccgtcag ttgggactgg tgcgtggaaa gacgctgttg
    17461 actgggtact cgaagaagct tgccaggctg taaacattcg catcaaagga gagtgagatg
    17521 accactatga acagagaaga acaaaagcaa gttctcatag atacagctaa tcacgtaatc
    17581 aatcgtgata atacttcacc atatagcgaa aatcttaggg aactggcacg aattgcactg
    17641 gcatcactgg cagcagtatc ggatgaacga gcagcctatg aattatttat ggagaagcgt
    17701 ttcggggaat ctgtagatcg ccgtagggca aaaaatggcg atagagaata catggcatgg
    17761 gatatggcgc ttggctggat tatctggtgt caccgcgctg ccatgctgca ttgtgccgaa
    17821 cctgcaagta agcatgaaga gttgccgctt gattatctcc aaggtcaaaa agatggtctt
    17881 gaatgggctg cgcagcttgc agaagcaaat cacccacaaa ctggcgactg gctttacgat
    17941 gacccgctgg atctggctaa agctatcaga aaaggtcctg acattcccga gttcgctgga
    18001 ccccatccgg taactccgga tggttggata agctgtagtg atcgaatgcc ggaagacacc
    18061 aaaatgttac tggcatttag tcaaggtgaa atcgtggccg catattggaa ctgggttgta
    18121 aatccaattg attacaaaaa atatagagct ttcacgtatt tatcaggata tatcttggat
    18181 gacgtaactc actggatgcc gctaccagaa ccgccgcagg aggttaaccg tggctaacct
    18241 gcaacttgcc gtcaaaggtg aatacttcga tgccatgatt cgcggggaga aaacggaaga
    18301 gtatcgcctg tgtaatgact actggaataa gcgaattatg ttccgggaat atgaccgtct
    18361 gattatcaca aagggatatc cgaagcgcga cgattccagc cgcagaattg acgtcccgta
    18421 tgaagggtat gaaatcaaga caatcacaca tacacacttc ggagataaac cggtaaaggt
    18481 gttcgcgata aaggtgaata tcggcagtga ttgacttcct cgcactcgcg gggatttctt
    18541 ttatctgaaa aggacagaat acatgattaa caatattgca aatcagtctt cgtttccggc
    18601 tgcaacaatc gacagccaga tgctgttgaa aatggtcaat gaggcacgga aattatgtag
    18661 tgaaaagccc gttcgcaata atgatttcat tgccagagtt aaggatgagc ttgaaggaga
    18721 gacctacgaa attttcgtag gtcagaaaaa cggcgcagaa atagaagtta taacaatgac
    18781 tttcaagcaa gccctgcgag tagccgcgcg tgagtcgaag gccgttcgtc gttcgctggt
    18841 cgataaactg gaatcaatgc aacagcagtt gcaacaaaaa accaccacga agaaatcacc
    18901 ggatggcctt gaagaattcc gtaaggcacg cgcattgaaa atgaccgtcg acacaatgaa
    18961 ggacctcttc gacttcctcc ctaaccttgc accggaagct aaacaggtcg ttgccgccag
    19021 cctggttaat cccgttgtag gcgctaacgt aatcccgcta ccgatgatca atgaacatta
    19081 ctattcggcg tcagaagttg gagcaaaact caaaatatcc gctaacaagg ttggtcgcat
    19141 tgccaatacc tacatgctca aaacagaaca atacgggaaa tggttcatcg acaaatcccc
    19201 gtattcggat aagcaggtag aaagctttcg atacaataat cggggagtac gaaagattga
    19261 agaaatcctg gacgcggaga acaaagcaga gttcggaacc tgatatcctc cactctccga
    19321 cgccgccaga atgacggcgc ggtggttaag catgaggcga aatttctgcc gggcggtatt
    19381 ttgttgagag atgagatacg tcaacttctc tttccgcttt ttcgaggctc catgccgtct
    19441 ggagacgaat ccatgaagac ggagttccac ccaggacagc agcgagacga acagccattt
    19501 caggagatat tgtcgcatta cctgaaacca gtcgctgaac agtggaaggc gcgacatcaa
    19561 gcgctcttgc cagttccctg attccaatat tctgatcttc cagaatatca gcaataattt
    19621 ctccgggatg aggggggtta aacatcgcca ttagtggtaa tcctcgtagt taaacgaagc
    19681 ggatctggag ttttttccct gtagcacggg cgaatttttt cagtgtggca aatgatgggc
    19741 cacttatacc tgatgcaaga ttgctttcca ttctggtgat cgcagtcgct tttgttccca
    19801 tacgttcagc aacttcagcc tgggttaagc cagcttcttt tcgtgctgcc agcatttcat
    19861 caagcagtgc gaattcatcg gcgatggcgt cgtattctgc tttaaaagca ggatcctcca
    19921 tccatttagc tgccatttca tcgtgcgtca tggttggtgg gttacgttta ccagtcatgc
    19981 ttaacctcct tcattctggt ttcagctttt ttgagttcag ccggtggggt tttctgagtc
    20041 ttcttgatga aactgtgcag catgatgatg cgttttcctg ccaaagtgca gtaaaaaacg
    20101 cgtgcgatgc catcacagcc tttaatccga agctcgaaaa gtccgtcacc gaaggcgctg
    20161 gtgtgaggtt ctccgagatt gctgccgtat accttcatac gttcaatgag atgttggtat
    20221 cgggcacgca tactcaatgg aagctgatcg acttccagcc ggacctcttc actgtagtat
    20281 tcgatagtgt agttcatgag aatgaacata acaaatttgt tatatgcatt caaggtctgt
    20341 gttgacgaca acacatatca aacagacagt catgctgtta gatcacggtt tggttgatgg
    20401 aaggttgaca actcataaga aattaaattt attaataaga ctcattaatt tttatgttag
    20461 a
//
LOCUS       DAZGAN020000083        20333 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGAN020000083 DAZGAN020000000
VERSION     DAZGAN020000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.083"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..618
                     /locus_tag="ACHKYO_RS16900"
                     /old_locus_tag="pgaptmp_003380"
     CDS             <1..618
                     /locus_tag="ACHKYO_RS16900"
                     /old_locus_tag="pgaptmp_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU4430168.1"
                     /translation="SFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNNAKLFRAGQM
                     KVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVASNDATAGGAI
                     QALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQ
                     EPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            696..2237
                     /gene="xylG"
                     /locus_tag="ACHKYO_RS16905"
                     /old_locus_tag="pgaptmp_003381"
     CDS             696..2237
                     /gene="xylG"
                     /locus_tag="ACHKYO_RS16905"
                     /old_locus_tag="pgaptmp_003381"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HGU4430169.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            2215..3396
                     /gene="xylH"
                     /locus_tag="ACHKYO_RS16910"
                     /old_locus_tag="pgaptmp_003382"
     CDS             2215..3396
                     /gene="xylH"
                     /locus_tag="ACHKYO_RS16910"
                     /old_locus_tag="pgaptmp_003382"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HGU4430170.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            3474..4652
                     /gene="xylR"
                     /locus_tag="ACHKYO_RS16915"
                     /old_locus_tag="pgaptmp_003383"
     CDS             3474..4652
                     /gene="xylR"
                     /locus_tag="ACHKYO_RS16915"
                     /old_locus_tag="pgaptmp_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HGU4430171.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(4760..5584)
                     /gene="bax"
                     /locus_tag="ACHKYO_RS16920"
                     /old_locus_tag="pgaptmp_003384"
     CDS             complement(4760..5584)
                     /gene="bax"
                     /locus_tag="ACHKYO_RS16920"
                     /old_locus_tag="pgaptmp_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HGU4430172.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            5904..7934
                     /gene="malS"
                     /locus_tag="ACHKYO_RS16925"
                     /old_locus_tag="pgaptmp_003385"
     CDS             5904..7934
                     /gene="malS"
                     /locus_tag="ACHKYO_RS16925"
                     /old_locus_tag="pgaptmp_003385"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU4430173.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            8112..9365
                     /gene="avtA"
                     /locus_tag="ACHKYO_RS16930"
                     /old_locus_tag="pgaptmp_003386"
     CDS             8112..9365
                     /gene="avtA"
                     /locus_tag="ACHKYO_RS16930"
                     /old_locus_tag="pgaptmp_003386"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HGU4430174.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(9516..9989)
                     /gene="ysaA"
                     /locus_tag="ACHKYO_RS16935"
                     /old_locus_tag="pgaptmp_003387"
     CDS             complement(9516..9989)
                     /gene="ysaA"
                     /locus_tag="ACHKYO_RS16935"
                     /old_locus_tag="pgaptmp_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU4430175.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQDNQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(10091..10939)
                     /gene="yiaJ"
                     /locus_tag="ACHKYO_RS16940"
                     /old_locus_tag="pgaptmp_003388"
     CDS             complement(10091..10939)
                     /gene="yiaJ"
                     /locus_tag="ACHKYO_RS16940"
                     /old_locus_tag="pgaptmp_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HGU4430176.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            11140..12138
                     /gene="yiaK"
                     /locus_tag="ACHKYO_RS16945"
                     /old_locus_tag="pgaptmp_003389"
     CDS             11140..12138
                     /gene="yiaK"
                     /locus_tag="ACHKYO_RS16945"
                     /old_locus_tag="pgaptmp_003389"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HGU4430177.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            12150..12617
                     /gene="yiaL"
                     /locus_tag="ACHKYO_RS16950"
                     /old_locus_tag="pgaptmp_003390"
     CDS             12150..12617
                     /gene="yiaL"
                     /locus_tag="ACHKYO_RS16950"
                     /old_locus_tag="pgaptmp_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGU4430178.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            12735..13208
                     /gene="yiaM"
                     /locus_tag="ACHKYO_RS16955"
                     /old_locus_tag="pgaptmp_003391"
     CDS             12735..13208
                     /gene="yiaM"
                     /locus_tag="ACHKYO_RS16955"
                     /old_locus_tag="pgaptmp_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HGU4430179.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            13211..14488
                     /gene="yiaN"
                     /locus_tag="ACHKYO_RS16960"
                     /old_locus_tag="pgaptmp_003392"
     CDS             13211..14488
                     /gene="yiaN"
                     /locus_tag="ACHKYO_RS16960"
                     /old_locus_tag="pgaptmp_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HGU4430180.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAL
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            14501..15487
                     /gene="yiaO"
                     /locus_tag="ACHKYO_RS16965"
                     /old_locus_tag="pgaptmp_003393"
     CDS             14501..15487
                     /gene="yiaO"
                     /locus_tag="ACHKYO_RS16965"
                     /old_locus_tag="pgaptmp_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HGU4430181.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            15491..16987
                     /gene="lyxK"
                     /locus_tag="ACHKYO_RS16970"
                     /old_locus_tag="pgaptmp_003394"
     CDS             15491..16987
                     /gene="lyxK"
                     /locus_tag="ACHKYO_RS16970"
                     /old_locus_tag="pgaptmp_003394"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HGU4430182.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLIDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYHNFSE
                     AQRDLRHPVRTLLPDMTAHQLYQKKYQRYQHLIAALQGFHARIKEHTL"
     gene            16984..17646
                     /gene="ulaD"
                     /locus_tag="ACHKYO_RS16975"
                     /old_locus_tag="pgaptmp_003395"
     CDS             16984..17646
                     /gene="ulaD"
                     /locus_tag="ACHKYO_RS16975"
                     /old_locus_tag="pgaptmp_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGU4430183.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            17639..18499
                     /gene="sgbU"
                     /locus_tag="ACHKYO_RS16980"
                     /old_locus_tag="pgaptmp_003396"
     CDS             17639..18499
                     /gene="sgbU"
                     /locus_tag="ACHKYO_RS16980"
                     /old_locus_tag="pgaptmp_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HGU4430184.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            18493..19188
                     /gene="araD"
                     /locus_tag="ACHKYO_RS16985"
                     /old_locus_tag="pgaptmp_003397"
     CDS             18493..19188
                     /gene="araD"
                     /locus_tag="ACHKYO_RS16985"
                     /old_locus_tag="pgaptmp_003397"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGU4430185.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGDNAYYGQ"
     gene            complement(19220..19321)
                     /locus_tag="ACHKYO_RS16990"
                     /old_locus_tag="pgaptmp_003398"
                     /pseudo
     CDS             complement(19220..19321)
                     /locus_tag="ACHKYO_RS16990"
                     /old_locus_tag="pgaptmp_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            19325..19588
                     /locus_tag="ACHKYO_RS16995"
                     /old_locus_tag="pgaptmp_003399"
                     /pseudo
     CDS             19325..19588
                     /locus_tag="ACHKYO_RS16995"
                     /old_locus_tag="pgaptmp_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19535..20275)
                     /gene="yiaT"
                     /locus_tag="ACHKYO_RS17000"
                     /old_locus_tag="pgaptmp_003400"
     CDS             complement(19535..20275)
                     /gene="yiaT"
                     /locus_tag="ACHKYO_RS17000"
                     /old_locus_tag="pgaptmp_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HGU4430186.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDHAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
BASE COUNT         4961 a         5188 c         5246 g         4938 t
ORIGIN      
        1 tctttcgata acgaaaaagt cggtgaactg caggcaaaag ccctggtcga tattgttccg
       61 caaggtaatt acttcctgat gggcggctcg ccggtagata acaacgccaa gctgttccgc
      121 gccggacaaa tgaaagtgtt aaaaccttac gttgattccg gaaaaattaa agtcgttggt
      181 gaccaatggg ttgatggctg gttaccggaa aacgcattga aaattatgga aaacgcgcta
      241 accgccaata ataacaaaat tgatgctgta gttgcctcaa acgatgccac cgcaggtggg
      301 gcaattcagg cattaagcgc gcaaggttta tcagggaaag tagcaatttc cggccaggat
      361 gcggatctcg caggtattaa acgtattgct gccggtacgc aaactatgac ggtgtataaa
      421 cctattacgt tgttggcaaa tactgccgca gaaattgccg ttgagttggg caatggtcag
      481 gaaccaaaag cagataccac actgaataat ggcctgaaag atgtgccctc ccgcctcctg
      541 acaccgatcg atgtgaataa aaacaacatc aaagacacgg tagttaaaga cggattccac
      601 aaagagagcg agctgtaagc gttacgcccc ggcgcggagc gggggcgtga tttctctcca
      661 tgccgcgtga atgaattggc ttaggtggag tcgttatgcc ttatctactt gaaatgaaga
      721 atattaccaa aaccttcggc agtgtgaagg cgattgataa cgtctgcttg cggttgaatg
      781 ctggcgaaat cgtctcactt tgtggggaaa atgggtctgg taaatcaaca ctgatgaaag
      841 tgctgtgtgg tatttatccc catggctcct acgaaggcga aattattttt gcgggagaag
      901 agattcaggc gagtcacatc cgcgataccg aacgcaaagg tatcgccatc attcaccagg
      961 aattggccct ggtgaaagaa ttgaccgtgc tggaaaatat cttcctgggt aacgaaataa
     1021 cccacaatgg cattatggat tatgacctga tgacgctacg ctgtcagaag ctgctcgcac
     1081 aggtcagttt atccatttca cctgataccc gcgttggcga tttagggctt gggcaacaac
     1141 aactggttga aattgccaag gcacttaata aacaggtgcg cttgttaatt ctcgatgaac
     1201 cgacagcctc attaactgag caggaaacgt cggttttact ggatattatt cgcgatctac
     1261 aacagcacgg tatcgcctgt atttatattt cgcacaaact caacgaagtc aaagcgattt
     1321 ccgatacgat ttgcgttatt cgcgacggac agcacattgg tacgcgtgat gctgccggaa
     1381 tgagtgaaga cgatattatc accatgatgg tcgggcgaga gttaaccgcg ctttacccta
     1441 atgaaccaca taccaccgga gatgaaatat tacgtattga acatctgacg gcatggcatc
     1501 cggtcaatcg tcatattaaa cgagttaatg atgtctcgtt ttccctgaaa cgtggcgaaa
     1561 tactgggtat tgccggactc gttggtgccg gacgtaccga gaccattcag tgcctgtttg
     1621 gcgtgtggcc cggacaatgg gaaggaaaaa tttatattga tggcaaacag gtagatattc
     1681 gtaactgtca gcaagccatc gcccagggga ttgcgatggt acccgaagac agaaagcgcg
     1741 acggcatcgt tccggtaatg gcggttggta aaaatattac cctcgccgca ctcaataaat
     1801 ttaccggtgg cattagccag cttgatgacg cggcagagca aaaatgtatt ctggaatcaa
     1861 ttcagcaact caaagttaaa acgtcgtccc ccgaccttgc tattggacgt ttgagcggcg
     1921 gcaatcagca aaaagcgatc ctcgctcgct gtctgttact taacccgcgc atcctcattc
     1981 ttgatgaacc taccaggggt atcgatattg gcgcgaaata cgagatctac aaattaatta
     2041 accaactcgt ccagcagggt attgccgtta ttgtcatctc ttccgaatta cctgaagtgc
     2101 tcggccttag cgatcgggta ctggtgatgc atgaagggaa actaaaagcc aacctgataa
     2161 atcataacct gactcaggag caggtgatgg aagccgcatt gaggagcgaa catcatgtcg
     2221 aaaagcaatc cgtctgaagt gaaattggcc gtaccgacat ccggtggctt ttccgggctg
     2281 aaatcactga atttgcaggt cttcgtgatg attgcagcta tcatcgcaat catgctgttc
     2341 tttacctgga ccaccgatgg tgcctactta agcgcccgta acgtctccaa cctgttacgc
     2401 cagaccgcga ttaccggcat cctcgcggta ggaatggtgt tcgtcataat ttctgctgaa
     2461 atcgaccttt ccgtcggctc aatgatgggg ctattaggtg gcgtcgcggc gatttgtgac
     2521 gtctggttag gctggccttt gccacttacc atcattgtga cgttggttct gggactgctt
     2581 ctcggtgcct ggaacggatg gtgggtcgcg taccgcaaag tcccttcatt tattgtcacc
     2641 ctcgcgggca tgttggcatt tcgcggcata ctcattggca tcaccaacgg cacgactgtt
     2701 tcccccacca gcgccgcgat gtcacaaatt gggcaaagct atctacctgc cagcactggc
     2761 ttcatcattg gcgcgcttgg cttaatggct tttgttggtt ggcaatggcg cggaagaatg
     2821 cgccgtcagg ctttgggttt gcagtctccg gcctctaccg cagtagtcgg tcgccaggct
     2881 ttaaccgcta tcatcgtatt aggcgcaatc tggctgttga atgattaccg tggcgttccc
     2941 actcctgttc tgctgctgac gttgctgtta ctcggcggaa tgtttatggc aacgcggacg
     3001 gcatttggac gacgcattta tgccatcggc ggcaatctgg aagcagcacg tctctccggg
     3061 attaacgttg aacgcaccaa acttgccgtg ttcgcgatta acggattaat ggtagccatc
     3121 gccggattaa tccttagttc tcgacttggc gctggttcac cttctgcggg aaatatcgcc
     3181 gaactggacg caattgcagc atgcgtgatt ggcggcacca gcctggctgg cggtgtggga
     3241 agcgttgccg gagcagtaat gggggcattt atcatggctt cactggataa cggcatgagt
     3301 atgatggatg taccgacctt ctggcagtat atcgttaaag gtgcgattct gttgctggca
     3361 gtatggatgg actccgcaac caaacgccgt tcttgatttt gataaaaatt ttctcaaagc
     3421 cggttacgta ttaccggttt tgagtttttg catgattcag caggaaaaga accatgttta
     3481 ctaaacgtca ccgcatcaca ttactgttca atgccaataa agcctatgac cggcaggtag
     3541 tagaaggcgt aggggaatat ttacaggcat cacaatcaga atgggatatt ttcattgaag
     3601 aagatttccg cgcccgcatt gataaaatca aggactggtt aggagatggc gtcattgccg
     3661 acttcgacga caaacagatc gagcaagcgc tggctgatgt cgacgtcccc attgttgggg
     3721 ttggcggctc gtatcacctt gcagaaagtt acccacccgt tcattacatt gccaccgata
     3781 actatgcgct ggttgaaagc gcatttttgc atttaaaaga gaaaggcgtt aaccgctttg
     3841 ctttttatgg tcttccggaa tcaagcggca aacgttgggc cactgaacgc gaatatgcat
     3901 ttcgtcagct tgtcgccgaa gaaaagtatc gcggagtggt ttatcagggg ttagaaaccg
     3961 cgccagagaa ctggcaacat gcgcaaaatc ggctggcaga ctggctacaa acgctgccac
     4021 cgcaaaccgg gattattgcc gttactgacg cccgggcgcg gcatattctg caagtatgtg
     4081 aacatctaca tattcccgta ccggaaaaat tatgcgtgat tggcatcgat aacgaagaac
     4141 tgacccgcta tctgtcgcgt gtcgcccttt cttcggtcgc tcagggcgcg cggcaaatgg
     4201 gctatcaggc ggcaaaactg ttgcatcgat tattagataa agaagaaatg ccgctacagc
     4261 gaattttggt cccaccagtt cgcgtcattg aacggcgctc aacagattat cgctcgctga
     4321 ccgatcccgc cgttattcag gccatgcatt acattcgtaa tcacgcctgt aaagggatta
     4381 aagtggatca ggtactcgat gcggtcggga tctcgcgctc caatcttgag aagcgtttta
     4441 aagaagaggt gggtgaaacc atccatgcca tgattcatgc cgagaagctg gagaaagcgc
     4501 gcagtctgct gatttcaacc accttgtcga ttaatgagat atcgcaaatg tgcggttatc
     4561 catcgctgca atatttctac tctgttttta aaaaagcata tgacacgacg ccaaaagagt
     4621 atcgcgatgt aaatagcgag gtcatgttgt agcgatgatg agaattgtcg gcgtcacatc
     4681 agggaactca gcacagccac ctgatgcgag caaaagcctt atccgaccga caaatcagat
     4741 aaggctcaag gaaatgcaat tacatatgcg ccgcgattaa ccgttggtta tcctggtaca
     4801 ttgcgaacag gtagttgtta taactcttcc ctttggtcga gtagcccttc agcttgtgaa
     4861 tcatcgctgt ggcagtcact tcctgatccg ctttacgcag ctgcgcacgc gatttacgga
     4921 acgaagagta agccgggtgc gtattcaggt tagtgacata ggcgctcacc gattctttga
     4981 cagaactaaa ctgtgagtac cctttcactt tacccggcgc attggtacaa cgtcctttca
     5041 tgcatttcat gccgaacaga ttgttattgt tgcgcgccag cttcgacgtt ccccaaccgc
     5101 tttctgctgc agccatcgtc gccaccatac tggtagggat aatgtctacg cgttcaagca
     5161 aggtattcca cgggattttt cgcgtattac cagaccactt caccttgtag cgtttggcga
     5221 tgtctttcag acgcgcacgc tcagcaggtg accattggcc ctgatactgt tttgaaatca
     5281 gccagttacg ttctgcagta atggctgcat tttggctggt aatgtaaggc attacggtcc
     5341 ggagaaacgc ctttttcctt ggtgttccgg aagggtattt tcgcaaatca ggaagtgaac
     5401 tactctttgc actattgcga gaatactctt gtttactgct tacctgttta ttactagctt
     5461 tagttaagtg ggacttttga ctcgctgttg ttgtgtgcgt cttcgctaac acctcactcg
     5521 aaaacaccag agtgagtaac ataagaatca tcgccccata tcgtcgtatg ggagtcaaaa
     5581 tcatcaggtc tcctggtcgg atttaatcat tccaacacct tatatttttc acaaatttga
     5641 gagttgaatc tcaaatcata tcaaaaatag ctgtcaagag caccccaagg aatagtccaa
     5701 atctgaaact atgtcacgtg ttaacgattc agattggcgc taaatcgcag aaaatgtggg
     5761 gtttatcgca aaattcagcc gttttttgcg cgagatcgct cacccttgct tctcatcctg
     5821 tggacttacc gctcagggat gagttttgtc tggcttatcg ctggcaaact gtctgaaatc
     5881 gcagcaataa ggactcatcc accatgaaac tcgccgcctg ttttctgaca ctccttcctg
     5941 gcttcgccgt tgccgccagc tggacttctc cggggttccc tgcctttagc gaacagggaa
     6001 cgggaacatt tgtcagccac gcgcagttgc ccaaaggtac gcgtccactc acgctaaatt
     6061 ttgaccaaca gtgctggcag cctgcagatg cgataaaact caatcagatg ctttccctgc
     6121 aaccttgtag caacacgccg cctcaatggc gattgttcag agacggcaaa tatacgctgc
     6181 aaatagacac ccgctccggt acgccaacat tgatgatttc catccagaac gccgccgaac
     6241 cggtagcaaa cctggtccgt gaatgcccga aatgggatgg attaccgctc acgctggatg
     6301 tcagcgccac tttcccggaa ggagccgccg tacgggatta ttacagccag caaattgcga
     6361 tagtgaagaa cggtcaaata acgttacaac ccgctgctac cagcaacggt ttactcctgc
     6421 tggaacgggc agaaactgac gcccctgccc ctttcgactg gcataacgcc acggtttact
     6481 ttgtgctgac agatcgtttc gaaaacggcg atcccagtaa tgaccagagt tacggacgtc
     6541 ataaagacgg tatggcggaa attggcactt ttcacggcgg cgatttacgc ggcctgacca
     6601 acaaactgga ttacctccag cagttgggcg ttaatgcttt atggataagc gccccatttg
     6661 agcaaattca cggctgggtc ggcggcggta caaaaggcga tttcccgcat tatgcctacc
     6721 acggttatta cacacaggac tggacgaatc ttgatgccaa tatgggcaac gaagccgatc
     6781 tacggacgct ggttgatagc gcacatcagc gcggtattcg tattctcttt gatgtcgtga
     6841 tgaaccacac cggctatgcc acgctggcgg atatgcagga gtatcagttt ggcgcgttat
     6901 atctttctgg tgacgaggtg aaaaaaacgc tgggtgaacg ctggagcgac tggaaacctg
     6961 ccgccgggca aacctggcat agctttaacg attacattaa tttcagcgac aaaacaggct
     7021 gggataaatg gtggggaaaa aactggatca gaacggatat cggcgattac gacaatcctg
     7081 gattcgacga tctcactatg tcgctggcct ttttgccgga tatcaaaacc gaatcaacta
     7141 ccgcttctgg tctgccggtg ttctataaaa acaaaacgga tactcacgct aaagtcatcg
     7201 aaggctttac acctcgcgat tacttaaccc actggttaag tcagtgggtc cgcgactatg
     7261 ggattgatgg ttttcgggtc gataccgcca aacatgttga gttgcccgcc tggcagcaac
     7321 tgaaaaccga agccagcgcc gcgcttcgcg aatggaaaaa agctaacccc gacaaagcat
     7381 tagatgacaa acctttctgg atgaccggtg aagcctgggg ccacggcgtg atgcaaagtg
     7441 actactatcg ccacggcttc gatgcgatga tcaatttcga ttatcaggag caggcggcga
     7501 aagcagtcga ttgtctggcg cagatggata cgacctggca gcaaatggcg gagaaattgc
     7561 agggtttcaa cgtgttgagc tacctctcgt cgcatgatac ccgtctgttc cgtgaagggg
     7621 gcgacaaagc agcagagtta ttactattag cgccaggcgc ggtacaaatc ttttatggcg
     7681 atgaatcctc gcgtccgttc ggtcctacag gttctgatcc gctgcaaggt acacgttcgg
     7741 atatgaactg gcaggatgtt agcggtaaat ctgccgccaa cgtcgcgcac tggcagaaaa
     7801 tcagccagtt ccgcgcccgc catcccgcaa ttggcgcggg caaacaaacg acactttcgc
     7861 tgaagcaggg ctacggcttt gttcgtgagc atggcgacga taaagtgctg gtcatctggg
     7921 ctgggcaaca gtgacttttc cggcttcccg ttcgtcagta cctcgggaag ccgccaacca
     7981 ggataaaatg tcagccctaa tcagcgttgc aggataaagc accgctcact ctccaacaga
     8041 ccgatttgca ccccagcaaa tgtagcgtta ttgttacctt ccttgctaca gagttcgaca
     8101 gatatcccgc tatgacattc tccctttttg gtgacaaatt tacccgccac tccggcatta
     8161 cgctgctgat ggaagatctg aacgacggtt tacgcacgcc tggcgcgatt atgctcggcg
     8221 gcggtaatcc ggcgcagatc ccggaaatgc aggactactt ccagacgcta ctgaccgaca
     8281 tgctggaaag tggcaaagcg actgatgcac tgtgcaatta cgacggtcca caggggaaaa
     8341 cggagctact cacactgctt gccggaatgc tgcgcgagaa gttgggttgg gatatcgaag
     8401 cacagaatat tgcactaaca aacggcagcc agagcgcgtt tttctactta tttaatctgt
     8461 ttgccggacg ccgtgccgat ggtcgggtca aaaaagtgct gttcccgctg gcaccggaat
     8521 acattggcta cgctgacgcc ggactggaag aagatctgtt tgtctctgcg cgtccgaata
     8581 ttgaactgct gccggaaggc cagtttaaat accacgtcga ttttgagcat ctgcatattg
     8641 gcgaagaaac cgggatgatt tgcgtctccc ggccgacgaa tccaacaggc aatgtgatta
     8701 ctgacgaaga gttgctgaag cttgacgcgc tggcgaatca acacggcatt ccgctggtga
     8761 ttgataacgc ttatggcgtc ccgttcccgg gtatcatctt cagtgaagcg cgcccgctat
     8821 ggaatccgaa tatcgtgctg tgcatgagtc tttccaagct ggggctgcct ggctcccgct
     8881 gcggcattat catcgccaat gaaaaaatca tcactgccat caccaatatg aacggcatta
     8941 tcagcctggc acctggcggt attggtccgg cgatgatgtg tgaaatgatt aagcgtaacg
     9001 atctgctgcg cctgtctgaa acagtcatca aaccgtttta ctaccagcgt gttcaggaaa
     9061 ctatcgccat cattcgccgc tatttaccgg aagatcgctg cctaattcat aaaccagaag
     9121 gagccatttt cctctggcta tggtttaagg atttgcccat tacgaccgag caactctacc
     9181 agcgcctgaa agcacgcggc gtgctgatgg tgccggggca caacttcttc ccgggactgg
     9241 ataaaccgtg gccgcatacg catcaatgta tgcgcatgaa ctacgtacca gagccggaga
     9301 aaattgaggc gggggtgaag attctggcgg aagagataga aagagcctgg gctgaaagtc
     9361 actaaaaatt gcctgatgcg ctacgcttat caggcctaca tgatttctgc aatatattga
     9421 atttggaaga atttgtaggc cggataaggc gtttacgccg catctggcat aaacaaagcg
     9481 cactattccg gcgttgagaa acgccggaaa acgtttcaaa caggctgctg ccgtagccga
     9541 tgccgctgca ctttctcgac atccatgcac tgcaacgcct ttgtcgggca ggcttcaaca
     9601 caggccggcc ccgtctcccg atgccagcac agatcgcact taatcgccct cgcctttttg
     9661 cgcgaagaaa cgacctccat cgcaccaaac gggcaagcca gcatacagct tttacagcca
     9721 atgcaacgtg tttgttcaac gaaaatatgc ccatgttcgc ggcttatcgc gtcaacaggg
     9781 cagacattcg cgcacggtgc atcttcacac tgatgacagg ctactgccgt ggtccagctg
     9841 tggtctttaa tgacacgaat acgggaaata aactcgtctg gtgacaacgc agcgcaatcc
     9901 tgattatcct gatgcgacac tgcgcaagcc acttcacagg tacggcaacc gatacatttc
     9961 gtcgcatccg caataataaa ccggttcatg atcactcctt tgcactaaaa cagcagagtg
    10021 ccagagagcg cgtccctact gctttgatcc aacgaaggaa atgttaaagt tttggcactt
    10081 gtccaaagcg ttatgtgatc gcgccctggt cgtccctgac agtaaatccc agttcattag
    10141 aaatagcctg cgcggtttca cgcagtggtt tcaggagatt tttctccccc acctgtttca
    10201 gacgtgatgt cgaaagcgaa atcgacacgg cgtacggcac ccgcccatga atattaaaca
    10261 ccggaacagc aatacaggag acgccgagtt cgttttcttc tctgtccatc gccgctccgc
    10321 tttcacgaat gtgcgccagt tcgtcgaaca tcgcgggcag ctcggtaatg gtattgcggg
    10381 ttaacggctg gatctcatgc tgatggtttt cccagtatga cttcacgtag tccgggtgac
    10441 caaacgccat gtagatcttg cccattgcgg aacagtagag cggcatatgc tggccaatat
    10501 aggcacgggt tcgcagcatc ccggttgtgg gttccagctt ataaatcaaa atagcgtgat
    10561 cgtcttcgcg gctggagaag ttaatggttt caccagtggc gatgttcagt gcctcaagat
    10621 gcggagcggc gatatgaatg atattcagcg aagacagcgc cttctgcccg acggcaataa
    10681 atttggtagt caggcggtaa ctccctgcgg cgggcgcggt ggtcacatag ccgcaggact
    10741 gtaatccctg caataagcga tggacggtac tcttatttaa accagccagc tccgaaagat
    10801 gcgccaacgg acaaccgttt ggatagttgc tcaaaatctc aatcagcatc aacccgcgaa
    10861 acagactctg gcttccggct ggacgctctt tttcctgcgc catctcgttc tctttttttc
    10921 ccatcacttc tttccccatt ttgtcgcgtc ctgatggtag cgcaaagtgt gccgtagttc
    10981 acgatctcga cagataattt ataaccactt gatttttatg tattttgaaa ttcatcaatc
    11041 agattgcttt gttaaaaagt gatcgatata tttgaaatca agtttcgcat attgaaattt
    11101 taagccaaaa aagcgatcaa aaaaacaagg aagcctggga tgaaagtgac atttgagcag
    11161 ttaaaagcag cctttaatcg ggtcttaatt tcacgcggcg ttgacagcga aacggctgac
    11221 gcctgtgcag agatgttcgc ccgcaccacc gaatccggcg tttattctca cggcgttaat
    11281 cgtttccctc gtttcattca acaactggaa aacggcgata tcattcctga tgcccaaccc
    11341 aaacgtataa ccagcctcgg cgcaattgaa cagtgggatg cccagcgttc gatcggcaac
    11401 ctgacggcga aaaagatgat ggatcgcgcc attgaactgg ctgccgatca cggtattggt
    11461 ctggtggcac ttcgtaatgc caaccactgg atgcgcggcg gcagctacgg ctggcaggcg
    11521 gcggaaaaag gctatattgg catttgctgg accaactcca tcgccgtaat gccgccgtgg
    11581 ggcgcaaaag agtgtcgcat tggcactaac ccgctgatcg tcgccattcc ttccacgccg
    11641 atcaccatgg tcgatatgtc gatgtcgatg ttctcttacg gcatgttaga agttaaccgc
    11701 ctggcgggcc gtcagctccc ggtcgatggt ggctttgatg atgagggcaa tttgaccaaa
    11761 gaacctggcg ttatcgagaa gaatcgccgc attttgccga tgggctactg gaaaggttct
    11821 ggcatgtcga ttgtgctgga tatgatcgct actctccttt ccgacggcgc atcggttgcc
    11881 gaagtcaccc aggacaacag cgacgaatac ggcatttcac aaatttttat tgccattgaa
    11941 gtggacaagc ttatcgacgg tcccacccgc gatgccaagc tgcaacgtat catggattac
    12001 gttactaccg ctgaacgcgc tgacgaaaac caggccatcc gcttacccgg ccacgaattt
    12061 actaccctgc tggccgaaaa ccgccgtaac ggtattaccg ttgatgacag cgtgtgggcc
    12121 aaaatccagg cgttataagg agataagtca tgatttttgg acatatcgcg cagcccaatc
    12181 cgtgccgttt gcccgccgcc attgaaaagg cgctcgattt tctgcgcgct accgatttca
    12241 acgccctgga gccgggcgtt gtcgaaatcg acggcaaaaa tatttatgcg caaattatcg
    12301 atttaaccac acgcgaagcg gtggaaaacc gtccggaagt gcatcgtcgg tatatcgata
    12361 ttcagtttct ggcgtggggt gaagagaaaa tcggcattgc tattgatacg ggaaataata
    12421 aagtcagcga atcactatta gagcagcgcg atattatttt ttatcatgac agtgaacatg
    12481 aatcatttat agaaatgata ccgggcagct acgcaatttt ctttccgcag gatgttcacc
    12541 gaccaggttg tattttgcaa acagcctctg agatacgaaa aattgtggtt aaagtcgcat
    12601 taacggcgct gaattaatcg aataatcgtc tacgccagaa cgactgggta atgttattgc
    12661 tctaactata ttaataattt aggttgctgc acatgcaact tgaattatga cagacatagc
    12721 ctcaaggaat agctatgaaa aaaatacttg aagcaatact ggcgattaat ctcgccgtac
    12781 tttcctgtat tgtgtttata aacattattt taagatatgg ttttcagaca agcattttat
    12841 ctgtagatga attgtcacgt tatttatttg tctggttaac atttattggc gcgattgtgg
    12901 cttttatgga taacgcccac gttcaggtca cctttctggt ggagaaactc tcccccgcat
    12961 ggcaacgacg agtcgctctg gttacccatt ccttaatctt atttatttgt ggcgcactgg
    13021 cctggggcgc aacgctaaaa acaattcagg actggagcga ttattcaccg atcctcggtt
    13081 tacccatcgg cctgatgtat gccgcttgtc tgcccaccag cctcgttatc gcattctttg
    13141 aactgcgtca tttatatcaa ctcatcacgc gtagcaattc tctaacgtcg ccaccgcaag
    13201 gagcttaatc atggctgtgc tgatttttct gggctgtctg ttgggtggta ttgctatcgg
    13261 tttgcctatt gcctgggcac tgttgttgtg cggggcggcg ttaatgttct ggctggacat
    13321 gtttgatgtc cagatcatgg cacaaacgct ggtgaacgga gccgatagct tctccctgct
    13381 ggcgattccg ttctttgtgc tggcgggtga aatcatgaat gcgggcggct tgtcaaaacg
    13441 cattgttgac ctgccgatga aactggtggg gcataaacca ggcgggctgg gctacgtcgg
    13501 cgtgctggca gctttgatta tggcgagcct ttctggttct gccgttgcgg ataccgccgc
    13561 cgttgccgct ctactggtgc cgatgatgcg cagcgccaac tacccggtca accgggcggc
    13621 ggggctgatt gcctctggcg gcattatcgc gccaattatt cccccttcca ttccgtttat
    13681 tatcttcggc gtttccagcg gattatccat cagtaagctg tttatggcgg gcattgcccc
    13741 tggcatgatg atgggcgcaa cgctgatgct tacctggtgg tggcaggcca gtcgccttaa
    13801 tttaccccgc cagcaaaaag caacgatgca ggaaatctgg cactcttttg tctccggtat
    13861 ctgggcgctg tttcttccag tcatcattat tggcgggttt cgctccggtc tgtttacccc
    13921 taccgaagcc ggggccgttg ctgctttcta tgcgctgttt gtcgccacag ttatttaccg
    13981 tgaaatgacc ttcgccacac tttggcatgt actcattggc gcggcgaaaa ccacctcagt
    14041 ggttatgttt ctggtggcct cggcacaagt ttccgcctgg ctgattacca ttgctgaact
    14101 gccgatgatg gtttctgatt tactgcaacc gttggtcgat tcaccgcgtc tgctgtttat
    14161 cgtcattatg gtggctatcc tgattgtcgg catggtcatg gatttaacgc cgacggtatt
    14221 aattcttacc ccggtattaa tgcctttagt taaagaggca ggaatcgatc cgatttattt
    14281 cggtgtcatg tttatcatta actgctcaat cggcttaatt acaccgccta tcggtaatgt
    14341 acttaacgtt atttcagggg tggcaaaact caaattcgat gatgcagtca gaggcgtttt
    14401 cccttacgtc ctggttttat attcattatt agtcgtattt gtttttattc ccgatctcat
    14461 catcctccct ttgaaatgga ttaattaaaa ggaaaatatt atgaaattac gctctgtaac
    14521 ctacgcatta ttcattgctg gcctggctgc attcagcaca tcttctctgg cggcgcaatc
    14581 tttacgtttc ggttatgaaa cctcacaaac cgactcgcaa catattgcgg cgaaaaaatt
    14641 caatgattta ttgcaggaga gaaccaaagg cgagctgaaa ttaaaattgt ttccggatag
    14701 caccctcggt aacgcgcagg cgatgatcag cggcgtacgt ggcggcacca tcgatatgga
    14761 aatgtccggc tcgaataact ttgccgggtt atcaccagtg atgaacttgc ttgatgtccc
    14821 tttcctgttc cgcgataccg ctcacgcgca taaaacgctc gacggcaaag tcggtgatga
    14881 tctgaaagcc tcacttgaag gtaaaggact aaaagtactg gcctactggg aaaacggctg
    14941 gcgcgatgtc accaactcgc gcgcaccggt taaaaccccc gccgacctga aagggctgaa
    15001 aattcgcacc aacaatagcc cgatgaatat cgccgcattc aaagtctttg gcgctaaccc
    15061 gatcccgatg ccatttgccg aagtctatac cgggctggaa acgcgcacta tcgacgctca
    15121 ggaacacccg atcaatgtcg tctggtcagc aaaatttttc gaagtgcaga agtacctttc
    15181 tctgacgcac cacgcctatt ccccgcttct ggtggtgatc aacaaagcga agtttgatgg
    15241 cttaaccccg gagttccagc aagcgctaat ttcatctgcg caggaagcgg gtaactacca
    15301 gcgcaaactg gttgccgaag atcagcaaaa aatcatcgac ggcatgaaag aagcgggcgt
    15361 ggaagtcatc accgatctcg accgcaaagc ctttagcgac gcactgggta ctcaggtccg
    15421 cgacatgttt gtaaaagatg ttccgcaggg tgctgatcta ctgaaagccg tggatgaggt
    15481 gcaataaacc atgacgcaat actggctggg gttagattgt ggcggtagct ggctgaaagc
    15541 cgggctgtat gaccgcgaag gccgggaggc aggcgtgcag cgcctgccgc tgtgcgcatt
    15601 aagcccgcag ccaggttggg cagagcgcga tatggcagaa ctgtggcaat gctgcatggc
    15661 tgtcattcgc gaactgctta cccattccgg cgttagcggg gaacaaattg tcggtatcgg
    15721 tatctccgca caaggaaaag gcttgttttt gctggataaa aacaacaagc cgctcggtaa
    15781 cgccattttg tcctcggatc gccgggcgat ggaaatcgtt cgtcgctggc aggaagatgg
    15841 catcccggaa aaactctacc cgttgacccg acaaaccttg tggaccgggc atccggtgtc
    15901 gctgttacgc tggctgaaag agcacgaacc ggaacgctac gcgcaaattg gctgcgtgat
    15961 gatgacgcac gactacctgc gctggtgttt aaccggcgtc aaaggctgtg aagagagcaa
    16021 tatttccgag tccaacctct acaacatgag tcttggggaa tatgacccgt gcctcatcga
    16081 ctggctgggg attgctgaaa tcaatcacgc cctgccgcct gttgtcggat ctgccgaaat
    16141 ctgcggggag atcaccgctc agacagccgt actgaccggt ctgaaagcgg gtacgcccgt
    16201 tgtcggcggc ctgtttgatg tggtttccac cgcactctgc gccgggatcg aagacgaatt
    16261 tacccttaat gcggtgatgg gcacctgggc ggtgaccagc ggcataaccc gcggtttacg
    16321 tgacggtgaa gcgcatccgt atgtctatgg tcgctacgtt aacgatggtg aatttatcgt
    16381 tcacgaagcc agccccacct cttccggcaa cctcgaatgg tttaccgcac agtggggaga
    16441 aatctcgttt gatgagatca atcaggccgt tgccagcttg ccgaaggctg ggggcgatct
    16501 ctttttcctg ccgttcctgt acggcagcaa cgccggactc gagatgacca gtggtttcta
    16561 cgggatgcag gccattcaca cccgcgcgca cctgttgcag gccatctatg aaggggtggt
    16621 gttcagccat atgacccacc tcaaccgaat gcgcgaacgt tttactgatg ttcacaccct
    16681 acgcgtcact ggcggcccgg cgcactccga tgtctggatg caaatgctgg cggacgtcag
    16741 cggtctgcgt atcgagctgc cgcaggtgga agaaaccggc tgctttggtg cggcccttgc
    16801 cgcccgcgtc ggcaccgggg tttatcacaa cttcagcgaa gcccaacgtg acttgcgaca
    16861 cccggtgcgc accctgctgc cagatatgac cgcccatcag ctttaccaaa aaaaatatca
    16921 acgttatcag catctcattg ccgcacttca gggctttcac gcccgcatta aggagcacac
    16981 attatgagcc gaccacttct gcaactggcc ctcgaccact catcacttga agccgcgcag
    17041 cgcgacgtga cgctgttaaa agacagcgtc gatatcgtcg aagcgggcac cattctctgt
    17101 ttaaacgaag ggcttggcgc ggtgaaagcc ttgcgcgaac agtgcccgga caaaatcatc
    17161 gttgctgact ggaaggtcgc cgacgctggt gaaacgctcg cgcaacaggc gtttggcgca
    17221 ggcgctaact ggatgaccat catctgcgcc gcgccgctcg cgacggtaga aaaaggccac
    17281 gcaatggcgc aacgctgcgg gggtgaaatt cagatagagc tgttcggtaa ctggacgctg
    17341 gacgacgccc gcgactggca tcgtattggc gtgcggcagg ccatttatca tcgcggtcgt
    17401 gatgcacagg ccagcgggca acagtggggc gaagccgatc tggcacgcat gaaggcgctt
    17461 tcagatatcg gccttgagct ttccattact ggcgggatta cccctgctga cctgccgctg
    17521 tttaaagata tccgcgtgaa agcgtttatt gccgggcggg cactggcagg cgcggcaaat
    17581 ccggcgcaag tggctggcga tttccatgcg caaatcgacg ctatctgggg aggtgcgcgt
    17641 gcgtaatcat ccgttaggga tttatgaaaa agcgctggcg aaagatctct cctggccgga
    17701 gcggctggtg ctggcaaaaa gctgcggttt tgattttgtc gaaatgtcgg tggatgaaac
    17761 cgacgaacgg ctctcacgtc ttgactggag cgccgcacaa aggacttcgc tggttgccgc
    17821 gatgatcgaa acaggtgttg ggatcccgtc gatgtgcctg tccgcacatc gtcgctttcc
    17881 ctttggtagc cgtgacgagg cagtgcgcga acgggcgcgg gaaatcatga gtaaagcgat
    17941 tcgcctggcg cgcgatctcg gcattcgcac catccagctg gcaggttatg acgtctatta
    18001 cgaagaccac gacgaaggca cccggcaacg ttttgctgaa gggctggcgt gggcagtcga
    18061 acaggcggcg gcatcacaag taatgctggc ggtggagatt atggatacag cgtttatgaa
    18121 ctccatcagc aaatggaaaa aatgggacga gatgctcgcc tcgccgtggt tcaccgtcta
    18181 cccggacgtc ggcaacctca gcgcctgggg caatgatgtt cccgccgaac tgaaactggg
    18241 cattgaccgt atcgccgcga tccacctgaa agacacccag ccagtcaccg ggcaaagccc
    18301 cggacagttc cgtgatgtgc cgtttggcga aggctgcgtc gatttcgttg gcatcttcaa
    18361 aacgctgcat gaactgaact atcgcggttc tttcctgatt gagatgtgga ccgaaaaagc
    18421 caaagagccg gtgctggaga tcattcaggc gcggcgctgg attgaagcgc gtatgcagga
    18481 ggctggattt atatgttaga gcaactgaaa gccgacgtgc tggcggcgaa tctggcgctt
    18541 cccgctcacc atctggtgac gttcacctgg ggcaatgtca gcgcggtaga cgacacgcgg
    18601 caatggatgg taatcaaacc ttccggcgtc gagtacgacg tgatgaccgc cgacgatatg
    18661 gtggtggttg agatagccag cggcaaggtg gtggaaggta gcaaaaagcc ctcgtccgat
    18721 acgccaacgc atctggcact ttaccgtcgc tatgccgaaa ttggtggcat tgtgcatacc
    18781 cactcgcgcc acgccaccat ctggtcacag gccgggctgg atctccccgc ctggggcacc
    18841 acgcacgctg attacttcta tggtgccatc ccctgcacac gcctgatgac cgcagaggag
    18901 atcaacggcg agtatgaata ccagaccggc gaagtgatca ttgaaacttt cgaagaacgc
    18961 ggcaggagtc cggcacaaat cccggcggta ctggtacatt ctcacggccc gttcgcctgg
    19021 ggaaaaaatg ccgccgatgc ggtgcataac gccgtggtgc tggaagagtg tgcctatatg
    19081 ggcctgttct cgcgccagct tgcgccgcag ctccctgcga tgcaaaacga actgctggat
    19141 aaacactacc tgcgtaagca tggagacaat gcctattacg ggcagtaatc cctcacgccg
    19201 gggcttcatc gccccggcac tacgaattga tatgttcctt gctgtaacgc cgcttccacg
    19261 ctgctggcgt taaaccggtg tgcttgcgaa aaatctgccg gaaatagcca acctcattaa
    19321 accgtattgc atggagcagg ccgacaacgt cgagtcactg cataacctgt tgctgcccgc
    19381 cgatgcgcca tttacgacat ttgaaggcaa gggattgttc agccataaga tcctgattca
    19441 gacgccaggt tcccggtatg aacagagcaa tccagagcaa caacctttgt ggcattacga
    19501 cggagcacca gccgcaccga catccaccgg tgaattaaaa acgccagctc acgccggtga
    19561 caaaggtaaa actctcatta cgatcaatca tcgggctgtc ggtaatctct tccggcagct
    19621 cgctgtatcc ggcgctcgcc atcaatacta cgtgctctcc tatcgggtat tttgccgtca
    19681 ggctgacata gggcacccag gcatcctgcg cggaataact tgccagaccg ctacgacggg
    19741 actcactctc tgaaatgcca tagtaatagt cactgaaatt ctcgtcataa tagagaacgc
    19801 ccagcgcagg tgtcagcgac aggcgaccta tctgcatttt gtggaaaacc gatagctccc
    19861 ccacccagcc gttgctgtta tccagaacgt ccgcagctgc agaggctttc acggttcccc
    19921 aacgctcgtg gtgataccag gcaacccccg ccatagccgt actatcacgc ttatcaagtt
    19981 gttgcatggc atgatcgtca ttatcggcag ggtcaaattc cagcggcatc caggatgcgg
    20041 tcaggctaag ttcatttttt tcactttgcg acagaataaa acctaacgtg gtctgacgaa
    20101 cataaaaaga atcaccttca taactaatca ggggaattgc ctttgtattt tcattataac
    20161 cgcgataagg cgattcatta taagccgcac cggcaccaat ggataattca gaagcagttg
    20221 cgcttgccat aaaaggcaac gcaaataacg ccacaatatt gcggttaatt aacatgtccg
    20281 gtattccatt ttaaaataag ataaaaaaga gtcggcatag taatgcttac aat
//
LOCUS       DAZGAN020000084        20221 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGAN020000084 DAZGAN020000000
VERSION     DAZGAN020000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.084"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            12..893
                     /gene="yieE"
                     /locus_tag="ACHKYO_RS17005"
                     /old_locus_tag="pgaptmp_003401"
     CDS             12..893
                     /gene="yieE"
                     /locus_tag="ACHKYO_RS17005"
                     /old_locus_tag="pgaptmp_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HGU4430187.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1098..2567
                     /gene="lysP"
                     /locus_tag="ACHKYO_RS17010"
                     /old_locus_tag="pgaptmp_003402"
     CDS             1098..2567
                     /gene="lysP"
                     /locus_tag="ACHKYO_RS17010"
                     /old_locus_tag="pgaptmp_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HGU4430188.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPNTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2860..4851
                     /gene="cirA"
                     /locus_tag="ACHKYO_RS17015"
                     /old_locus_tag="pgaptmp_003403"
                     /pseudo
     CDS             2860..4851
                     /gene="cirA"
                     /locus_tag="ACHKYO_RS17015"
                     /old_locus_tag="pgaptmp_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(4883..5719)
                     /gene="yeiG"
                     /locus_tag="ACHKYO_RS17020"
                     /old_locus_tag="pgaptmp_003404"
     CDS             complement(4883..5719)
                     /gene="yeiG"
                     /locus_tag="ACHKYO_RS17020"
                     /old_locus_tag="pgaptmp_003404"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU4430189.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5977..6645
                     /gene="folE"
                     /locus_tag="ACHKYO_RS17025"
                     /old_locus_tag="pgaptmp_003405"
     CDS             5977..6645
                     /gene="folE"
                     /locus_tag="ACHKYO_RS17025"
                     /old_locus_tag="pgaptmp_003405"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGU4430190.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6662..7819
                     /gene="yeiB"
                     /locus_tag="ACHKYO_RS17030"
                     /old_locus_tag="pgaptmp_003406"
     CDS             6662..7819
                     /gene="yeiB"
                     /locus_tag="ACHKYO_RS17030"
                     /old_locus_tag="pgaptmp_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HGU4430191.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            7961..9001
                     /gene="galS"
                     /locus_tag="ACHKYO_RS17035"
                     /old_locus_tag="pgaptmp_003407"
     CDS             7961..9001
                     /gene="galS"
                     /locus_tag="ACHKYO_RS17035"
                     /old_locus_tag="pgaptmp_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HGU4430192.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMTKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9281..10279
                     /gene="mglB"
                     /locus_tag="ACHKYO_RS17040"
                     /old_locus_tag="pgaptmp_003408"
     CDS             9281..10279
                     /gene="mglB"
                     /locus_tag="ACHKYO_RS17040"
                     /old_locus_tag="pgaptmp_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HGU4430193.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10340..11860
                     /gene="mglA"
                     /locus_tag="ACHKYO_RS17045"
                     /old_locus_tag="pgaptmp_003409"
     CDS             10340..11860
                     /gene="mglA"
                     /locus_tag="ACHKYO_RS17045"
                     /old_locus_tag="pgaptmp_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HGU4430194.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11876..12886
                     /gene="mglC"
                     /locus_tag="ACHKYO_RS17050"
                     /old_locus_tag="pgaptmp_003410"
     CDS             11876..12886
                     /gene="mglC"
                     /locus_tag="ACHKYO_RS17050"
                     /old_locus_tag="pgaptmp_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HGU4430195.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12957..14192)
                     /gene="preA"
                     /locus_tag="ACHKYO_RS17055"
                     /old_locus_tag="pgaptmp_003411"
     CDS             complement(12957..14192)
                     /gene="preA"
                     /locus_tag="ACHKYO_RS17055"
                     /old_locus_tag="pgaptmp_003411"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HGU4430196.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14186..15424)
                     /gene="preT"
                     /locus_tag="ACHKYO_RS17060"
                     /old_locus_tag="pgaptmp_003412"
     CDS             complement(14186..15424)
                     /gene="preT"
                     /locus_tag="ACHKYO_RS17060"
                     /old_locus_tag="pgaptmp_003412"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HGU4430197.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(15743..15982)
                     /locus_tag="ACHKYO_RS17065"
                     /old_locus_tag="pgaptmp_003413"
     CDS             complement(15743..15982)
                     /locus_tag="ACHKYO_RS17065"
                     /old_locus_tag="pgaptmp_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HGU4430198.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(15985..16704)
                     /gene="sanA"
                     /locus_tag="ACHKYO_RS17070"
                     /old_locus_tag="pgaptmp_003414"
     CDS             complement(15985..16704)
                     /gene="sanA"
                     /locus_tag="ACHKYO_RS17070"
                     /old_locus_tag="pgaptmp_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HGU4430199.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(16854..17738)
                     /gene="cdd"
                     /locus_tag="ACHKYO_RS17075"
                     /old_locus_tag="pgaptmp_003415"
     CDS             complement(16854..17738)
                     /gene="cdd"
                     /locus_tag="ACHKYO_RS17075"
                     /old_locus_tag="pgaptmp_003415"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGU4430200.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(17868..18563)
                     /locus_tag="ACHKYO_RS17080"
                     /old_locus_tag="pgaptmp_003416"
     CDS             complement(17868..18563)
                     /locus_tag="ACHKYO_RS17080"
                     /old_locus_tag="pgaptmp_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HGU4430201.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(18560..18958)
                     /locus_tag="ACHKYO_RS17085"
                     /old_locus_tag="pgaptmp_003417"
     CDS             complement(18560..18958)
                     /locus_tag="ACHKYO_RS17085"
                     /old_locus_tag="pgaptmp_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HGU4430202.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19197..20144
                     /gene="dusC"
                     /locus_tag="ACHKYO_RS17090"
                     /old_locus_tag="pgaptmp_003418"
     CDS             19197..20144
                     /gene="dusC"
                     /locus_tag="ACHKYO_RS17090"
                     /old_locus_tag="pgaptmp_003418"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HGU4430203.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         4941 a         5003 c         5277 g         5000 t
ORIGIN      
        1 ggtaagcgac tatgcacatc accctccggc agttggaagt ttttgcagaa gtattgaaaa
       61 gtggatcaac cacccaggcg tcggtgatgc tggcgttgtc gcaatcagca gtgagcgcag
      121 ccttgaccga cctggaaggg cagcttggcg tgcaactgtt tgatcgcgtg gggaaaagac
      181 tggttgttaa tgaacacggg cggctgctct atccgcgtgc gttggcattg cttgaacagg
      241 cggttgaaat cgaacaactg tttcgcgaag acaacggcgc gattcgtatc tatgccagta
      301 gtaccatcgg taactacatt ctgcctgcag ttatcgcccg ttatcgccat gattatccgc
      361 agttgccgat tgaacttagc gttgggaaca gccaggacgt gatgcaagcg gtgctggatt
      421 tccgcgttga tattggtttt attgaaggac cgtgccacag cactgaaatt atttctgaac
      481 cgtggctgga agacgagctg gtggttttcg ccgcgccgac ttcgccgttg gcccgtggtc
      541 cggtcacctt agagcagctg gccgctgcgc cgtggatcct gcgtgaacgc ggttccggca
      601 cgcgggagat tgtcgattat ctattgctgt cacatttacc gaagtttgag atggcgatgg
      661 aattaggtaa ctccgaggca atcaaacatg cggtgcgtca tgggttggga attagttgcc
      721 tgtcgcgacg ggtgattgaa gatcaattgc aggcaggcac attaagtgaa gttgcggtcc
      781 ctctgccgcg cctgatgcgt acgttgtggc gtatacatca tcggcaaaaa cacctttcca
      841 acgcgctacg gcgctttctg gactattgcg atcccgcgaa tgtaccgcgt taagttgctg
      901 tacaagaaca tgctggtgct gtgtcgattt cgtgacgcag cgccctcagc atgcattcgc
      961 cagaaaagag attggctgct ttacttataa tccctgggcg atcatgaagg tgtcttataa
     1021 ccgtgtattt ctgccggaag gattgccaat cgtctgctac aatcgcgcct catttttaag
     1081 atggatagca tttttgtatg gtttccgaaa ctaaaaccac agaagcgccg ggcttacgcc
     1141 gtgaattaaa ggcgcgtcac ctgacgatga ttgccattgg cggttccatc ggtacaggtc
     1201 tttttgttgc ctctggcgca acgatttctc aggcaggtcc gggcggggca ttgctctcgt
     1261 atatgctgat tggcctgatg gtttacttcc tgatgaccag tctcggtgaa ctggctgcat
     1321 atatgccggt ttccggttcg tttgccactt acggtcagaa ctatgttgaa gaaggctttg
     1381 gcttcgcgct gggctggaac tactggtaca actgggcggt gactatcgcc gttgacctgg
     1441 ttgcagctca gctggtcatg agctggtggt tcccgaatac accgggctgg atctggagtg
     1501 cgttgttcct cggcgttatc ttcctgctga actacatctc agttcgtggc tttggtgaag
     1561 cggaatactg gttctcactg atcaaagtca cgacagttat tgtctttatc atcgttggcg
     1621 tgctgatgat tatcggtatc ttcaaaggcg cgcagcctgc gggctggagc aactggacaa
     1681 tcggcgaagc gccgtttgct ggtggttttg cggcgatgat cggcgtagct atgattgtcg
     1741 gcttctcttt ccagggaacc gagctgatcg gtattgctgc aggcgagtcc gaagatccgg
     1801 cgaaaaacat tccacgcgcg gtacgtcagg tgttctggcg aatcctgttg ttctatgtgt
     1861 tcgcgatcct gattatcagc ctgattattc cgtacaccga tccgagcctg ctgcgtaacg
     1921 atgttaaaga catcagcgtt agtccgttca ccctggtgtt ccagcacgcg ggtctgctct
     1981 ctgcggcggc ggtgatgaac gcagttattc tgacggcggt gctgtcagcg ggtaactccg
     2041 gtatgtatgc gtctactcgt atgctgtaca ccctggcgtg tgacggtaaa gcgccgcgca
     2101 ttttcgctaa actgtcgcgt ggtggcgtgc cgcgtaatgc gctgtatgcg acgacggtga
     2161 ttgccggtct gtgcttcctg acctccatgt ttggcaacca gacggtatac ctgtggctgc
     2221 tgaacacctc cgggatgacg ggttttatcg cctggctggg gattgccatt agccactatc
     2281 gcttccgtcg cggttacgta ttgcagggac acgacattaa cgatctgccg taccgttcag
     2341 gtttcttccc actggggccg atcttcgcat tcattctgtg tctgattatc actttgggcc
     2401 agaactacga agcgttcctg aaagatacca ttgactgggg cggcgtagcg gcaacgtata
     2461 ttggtatccc gctgttcctg attatttggt tcggctacaa gctgattaaa ggaactcact
     2521 tcgtacgtta cagcgaaatg aagttcccgc agaacgataa gaaataagtt tcctcccttc
     2581 cttgctaagc cctctcaacc gagagggctt tttcaattcc atttccctga caaatcatgc
     2641 ggatataaaa tttaacattt ggattgataa ttgttatcgt ttgcattatc gttacgccgc
     2701 aatcaaaaaa ggctgacaaa tcagaggctg ttccggcttt ctgggatgat cacctgcata
     2761 caaataagtc caccgcgatg ctgccgtacg caaggggacg tgaagaagat gtgagcgata
     2821 acccatttta ttttcgtagt tacctcatgg agatatggaa tgtttaggtt gaaccctttc
     2881 gtacgggtcg ggctgtgttt gtccgctatt tcttgtgcat ggcctgtgtt agcggtcgat
     2941 gatgatggcg aaacgatggt tgtcactgca tcttccgttg aacaaaacct caaagatgca
     3001 cccgccagta tcagcgtcat tacccaggaa gacctgcagc gaaaaccggt acagaatctg
     3061 aaggatgtcc tcaaagaagt gcctggcgta caactgacga acgaagggga taaccgtaag
     3121 ggcgttagta ttcgtggtct ggacagcagc tacaccctga ttctcgtcga cggtaaacgc
     3181 gttaactccc gcaatgccgt cttccgccac aatgatttcg atctgaactg gatcccggtc
     3241 gattccatcg aacgtattga agtggttcgt ggcccgatgt cgtcgctata cggctccgat
     3301 gcgctcggcg gtgtagtgaa tatcatcacc aaaaaaatcg gtcagaaatg gtcgggtacc
     3361 gttaccgtcg ataccaccat tcaggaacat cgcgatcgcg gtgataccta taacggtcag
     3421 ttctttacca gtggaccatt aattgatggt gtgctgggaa tgaaagctta cggcagcctg
     3481 gcaaaacgtg aaaaggatga cccgcaaaac tcaacgacca ccgataccgg agaaacgccg
     3541 cgtattgaag gattctccag ccgcgacggc aatgtcgaat ttgcctagac accgaatcaa
     3601 aatcacgatt ttactgccgg atacggtttc gaccgtcagg atcgtgattc cgactcgctg
     3661 gacaaaaacc gcctggaacg ccagaactac tccgtcagcc ataatgggcg ttgggattac
     3721 ggcaccagcg aactgaaata ctacggtgag aaagtcgaga acaaaaaccc tggcaacagc
     3781 agcccgataa cttccgaaag caatacggtc gacggcaaat acacgttgcc gctgacggcg
     3841 attaatcagt ttctcacggt tggcggtgaa tggcgtcacg acaaacttag cgatgcggtg
     3901 aacctgaccg ggggaaccag ctccaaaacg tctgccagcc agtacgcgct gtttgtggaa
     3961 gatgaatggc ggatcttcga gccgctggcg ctgacgaccg gcgtgcgtat ggacgatcac
     4021 gaaacctacg gtgaacactg gagtccgcgt gcctacctgg tttataacgc caccgacacc
     4081 gtaacggtga aagggggctg ggcgacggca tttaaagcac cttctctgtt gcaacttagc
     4141 cctgactgga cgagcaattc ctgccgtggc gcatgtaaga ttgtgggtag cccggatctg
     4201 aaaccagaaa ccagcgaaag ttgggagctg gggctttact acatgggtga agaaggctgg
     4261 ctggaagggg ttgaatccag cgttaccgtt ttccgtaacg atgtgaaaga tcgtatcagc
     4321 atcagccgta cgtctgacgt caacgctgca ccgggctacc aaaactttgt tggttttgag
     4381 acgggcgcta acggacggcg cataccggta tttagctact acaacgttaa caaagctcgt
     4441 attcagggcg tggaaaccga actgaaaatt ccgttcaacg atgaatggaa actgtcgatc
     4501 aactacacct acaacgatgg tcgtgatgtc agcaacggcg aaaacaaacc gctatccgat
     4561 ctgccgttcc atactgctaa cggtacgctg gactggaaac cgctggcgct ggaagactgg
     4621 tcattctatg tttctggtca ctataccggg cagaaacgcg ccgacagcgc gacggctaaa
     4681 acaccgggcg gttataccat ctggaatacc ggcgcggcct ggcaggtgac taaagacgtc
     4741 aaactgcgcg caggcgtgct gaaccttggc gacaaggatc tcagtcgtga cgactacagc
     4801 tataacgaag acggacgtcg ttactttatg gcagtggatt atcgcttctg atgagaagat
     4861 gcccggcgaa ccgggcggac tttcacttca gtaaatactg cgcatggaag cgcaggtgat
     4921 cctctataaa agaggcgatg aagtagtaac tgtgatcata tcccggctga atacgcagcg
     4981 tcatcggcca cgctttctgg cgcgcggctt ctgccagtac cgcaggttgc aactggtcgg
     5041 caagaaactg atcattatcg ccctgatcga taagcgtcgg gatcgcatcc tgcgcgttac
     5101 tggcatacat cagcgcgcaa ctgtcccatt ccagccatgc atttttgtcc tcacctaaat
     5161 agctgctaaa cgctttgatt ccccacggga cgctgcacgg attcacaatt ggcgcaaagg
     5221 ccgaaacgct ggtgtattta cccggatttt tcagcgccat aatcagcgca ccgtgaccac
     5281 ccattgagtg accgctaatg gcgcagcggt cgctgacatt aaattgcgac tgaaccagcg
     5341 ccggtaattc atcgcgcaga taatcataca tccggtaatg cgtcgcccac ggcggttgcg
     5401 tggcattaag ataaaagcct gcgccctggc ctaaatcgta gccatcgtcg ttggcaacct
     5461 tttcgccgcg cgggctggtg tctggcatca ccagtacaat ccccagttct gccgccacgc
     5521 gctgggcacc cgccttggtg gtgaagttct cgtcattgca ggttaatccg gaaagccagt
     5581 acagcactgg tggcggggtg tgatcacgag ggggtgggag aaagatactg aacgtcatcg
     5641 ggcagtttaa ggtactggag tcgtgtcgcc agcgttgctg ccagccttca aaacagcggt
     5701 gctcttcgag catttccatg cgtggctcct tgttgtgttg tttgcaattt cttcatcata
     5761 ccgtttattc aatgtgctgt gagtaacttt cacttccgta tttgcataac gatgttttaa
     5821 catctgctga tgaaaggcag cggcaattac aataattatc gctgtgaata ctggattatg
     5881 tgcgccgcct cacgcacaat aatcaggctg taaatcagct taataacttt gcccccacgc
     5941 agggcggagg cgtcacacct gcaggagaaa tcataaatgc catcactcag taaagaagcg
     6001 gccctggttc atgaagcgtt agttgcgcga ggactggaaa caccgctgcg cccgcccgtg
     6061 catgaaatgg ataacgaaac gcgcaaaagc cttattgctg gtcatatgac cgaaatcatg
     6121 cagctgctga atctcgacct ggctgatgac agtttgatgg aaacgccgca tcgcatcgct
     6181 aaaatgtatg tcgatgaaat tttctccggt ctggattacg ccaacttccc gaaaatcacc
     6241 ctcattgaaa acaaaatgaa ggtcgatgaa atggtcaccg tgcgcgatat cactctgacc
     6301 agtacctgtg aacaccattt tgttaccatc gatggcaaag cgacggtggc ttatatcccg
     6361 aaagattcgg tgatcggtct gtcaaaaatt aaccgtatcg tgcagttctt tgcccagcgt
     6421 ccgcaggtgc aggaacgtct gacgcagcaa attcttattg cgctacaaac gctgctgggc
     6481 accaataacg tggctgtctc gatcgacgcg gtgcattact gcgtgaaggc gcgtggcatc
     6541 cgcgatgcaa ccagtgccac aacaacgacc tctcttggtg gattgttcaa atccagtcag
     6601 aatacgcgcc acgagtttct gcgcgctgtg cgtcatcaca actgattaaa aggcaggaac
     6661 catggagcgc aacgtcacgc tcgattttgt tcgcggcgtc gccattctgg ggatcctgct
     6721 attaaacatc agcgcctttg ggctaccaaa ggcggcttat ctcaatcccg cctggtacgg
     6781 tgctattacg ccgcaggatg catggacctg ggcatttctt gatctcatcg gccaggtgaa
     6841 attcctcacg ctttttgcgc tgctgtttgg tgctggactg caaatgttgc tgccccgtgg
     6901 cagacgctgg atccagtcgc ggttaacgct gttagtctta ttgggcttta ttcacggttt
     6961 attgttctgg gacggcgata ttctgctggc ttacgggctg gtgggcttaa tctgctggcg
     7021 gctggtgcgc gatgcgccat cggtaaaaag cctgtttaat accggcgtca tgctttatct
     7081 ggtggggctt ggcgttttgc tgttattggg gctgatttcc gacagccaga ccagccgcgc
     7141 ctggacgccg gatgcatcgg ctattttgta tgaaaaatac tggaagcttc acggcggcgt
     7201 tgatgcgatc agtaatcgtg ccgatggtgt tggcaacagt ttactggcac tgggcgcaca
     7261 gtatggctgg caactggctg ggatgatgct cattggtgcg gcgctgatgc gcagcggctg
     7321 gctgaaaggg cagttcagct tacgtcacta tcgtcgtact ggttttgtgc tggtggcgat
     7381 tggggtgacc attaaccttc ctgccatcgc cctgcaatgg cagctggact gggcgtatcg
     7441 ctggtgtgcc ttcttacttc aaatgccgcg ggaactgagt gcgccgtttc aggcgattgg
     7501 ctatgcgtcg ctgttttatg gcttctggcc gcaattaagc cgctttaagc tggtgctggc
     7561 aatcgcctgc gtcggacgga tggcgctgac caactatcta ttgcaaacgc tgatttgtac
     7621 cacgcttttt taccacctcg gtttgtttat gcattttgat cgcctggagc tgctggcgtt
     7681 tgttattccg gtatggctgg cgaatatcct cttctctgtt atctggctgc gttacttccg
     7741 ccaggggccg gtggaatggc tctggcgtca gttaactttg cgtgctgccg gaacggcaat
     7801 atctaaaaca tcaagataac gatctggatc acattcgtta acaaaacggc tgtaaccgtt
     7861 tccattgctg tgactcgatt cacgaagtcc tgtattcagt gctgacaaaa tagccgccag
     7921 caagcagtca tttactgcaa tctcataaca ggtagtgaat atgatcacca ttcgtgatgt
     7981 agcgcgtcag gctggcgtct ctgtggcaac ggtttcccgg gtgctcaata acagcacgct
     8041 ggtcagtgcc gacacgcgtg aagcagtaat gaaagccgtg agtgagctgg attatcggcc
     8101 aaatgccaat gcccaggcgc tggcaactca ggttagcgat accattggcg tggtggtgat
     8161 ggacgtttct gatgcttttt tcggcgcgct ggtaaaagcg gtggatctgg tcgcccagca
     8221 gcatcagaaa tacgtgctaa tcggcaatag ctatcatgaa gcggaaaaag agcgtcacgc
     8281 cattgaggtg ttaattcgcc agcgttgtaa tgcgttgatt gttcactcaa aagcattgag
     8341 tgacgatgaa ctggcgcaat ttatggataa cattcccggt atggtgttaa tcaaccgcgt
     8401 tgtgccgggg tacgcccatc gttgcgtttg cctggataat ctcagcggtg cccgaatggc
     8461 gacgcgcatg ttgctgaata acggtcatca acgtattggt tatctttctt ccagccacgg
     8521 cattgaagat gacgccatgc gtaaagcagg ctggatgagt gcgttgaaag agcaggatat
     8581 tattccgccg gaaagctgga ttggcactgg tacgccggac atgccgggcg gtgaggcggc
     8641 gatggttgaa ctgctggggc gcaatctaca acttaccgct gtatttgctt ataacgacaa
     8701 tatggccgct ggcgcactga cagcattaaa agataatggc attgcgattc cgttacatct
     8761 ctcaatcatc ggtttcgatg atattcccat cgcccgttac accgacccgc aattaacgac
     8821 cgtgcgttat cccattgctt caatgactaa attagccacc gaactggcct tgcagggggc
     8881 agcaggcaat attgatcctc gtgccagcca ctgttttatg ccgacgttag tgcgtcgtca
     8941 ttctgtcgca acgcgccaga atgcggcggc gatcactaac tcaacaaatc aggcgatgta
     9001 accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc tatgattgca
     9061 ccgttttaac gttgtaaccc gtatgtaaca gtgaataatc actttcaccg aggtaatagc
     9121 gtcataacaa caattaaagc cgttttctgg agcgttaccg ggcatggaag aacgaatttt
     9181 aaaaagtgag cttcggcgtt cagtaacact tcattaactc tactgccccg ccgagcattt
     9241 atctcaagca ctaccctgca taagaaaaac cggagatacc atgaataaga aggtgttaac
     9301 cctgtctgct gtgatggcca gcatgttatt cggtgccgct gcacacgctg ctgatactcg
     9361 cattggtgta acaatctata agtacgacga taactttatg tctgtagtgc gcaaagctat
     9421 tgagcaagat gcgaaagccg cgccagatgt tcagctgctg atgaatgatt ctcagaatga
     9481 ccagtccaag cagaacgatc agatcgacgt attgctggcg aaaggggtga aggcactggc
     9541 aatcaacctg gttgacccgg cagctgcggg tacggtgatt gagaaagcgc gtgggcaaaa
     9601 cgtgccggtg gttttcttca acaaagaacc gtctcgtaag gcgctggata gctacgacaa
     9661 agcctactac gttggcactg attccaaaga gtccggcatt attcagggcg atttgattgc
     9721 taaacactgg gcggcgaatc agggttggga tctgaacaaa gacggtcaga ttcagttcgt
     9781 actactgaaa ggtgagccgg gccatccgga tgcagaagca cgtaccactt acgtgattaa
     9841 agagctgaac gataaaggca tcaaaactga acagttacag ttagataccg caatgtggga
     9901 caccgctcag gcgaaagata agatggacgc ctggctgtct ggcccgaacg ccaacaaaat
     9961 cgaagtggtt attgctaaca acgatgcgat ggcaatgggt gcggttgaag cgctgaaagc
    10021 acacaacaag tccagcattc cggtgtttgg cgtcgatgcg ctgccagaag ctctggcgct
    10081 ggtgaaatcc ggtgcactgg cgggcaccgt actgaacgat gctaacaacc aggcgaaagc
    10141 gacctttgat ctggcgaaaa acctggccga tggcaaaggt gcggctgatg gcaccaactg
    10201 gaaaatcgac aacaaagtgg tccgcgtacc ttatgttggc gtagataaag acaacctggc
    10261 tgaattcagc aagaaataag actgattggg cgcagtcgtt tactgcgccc ttattgtatg
    10321 gccaacaagg taaaattata tggtcagctc aacgactccg tcttccgggg aatacttgtt
    10381 ggaaatgagc ggtatcaaca agtcctttcc tggtgttaag gcacttgata acgttaattt
    10441 aaaagtccgg ccacattcta tccatgcatt aatgggggaa aacggtgcag gaaaatcgac
    10501 attattaaaa tgcctgtttg gtatttatca aaaagactcc ggcaccattt tattccaggg
    10561 taaagagatc gatttccatt ccgccaaaga ggcactggaa aatggtattt cgatggtaca
    10621 ccaggagtta aacctggtat tacaacgttc ggtgatggat aacatgtggc tggggcgata
    10681 tcccaccaaa ggcatgtttg tcgatcagga caaaatgtac cgcgaaacca aagcgatttt
    10741 tgatgaactg gatattgata tcgatccgcg tgcgcgcgtc ggcacattat ccgtttcgca
    10801 aatgcagatg atcgaaatcg ccaaagcgtt ttcctataac gcgaaaattg tgattatgga
    10861 tgaaccgact tcttcgttaa ccgaaaaaga ggtcaatcat ctgttcacta ttattcgtaa
    10921 attaaaagag cgcggctgcg gtattgttta tatctcgcat aaaatggaag aaatcttcca
    10981 gttatgtgat gaagttaccg tattgcgcga cggtcagtgg atcgccaccg aaccgctggc
    11041 aggactgacg atggacaaga tcatcgccat gatggttggg cgttctctta atcagcgttt
    11101 tcctgacaaa gaaaacaagc cgggcgaagt catcctcgag gtgcgtaacc taacgtcact
    11161 gcgccagccg tcgattcgcg atgtctcgtt tgatctgcat aaaggggaga tcctcggtat
    11221 tgccgggctg gtgggggcga aacgtaccga tattgttgag acgttatttg gtattcgcga
    11281 gaaatcggct ggcaccatta cgttgcacgg caaacagatc aataaccata atgccaacga
    11341 agccataaac cacggatttg cactggtaac tgaggagcgc cgctcaacgg gaatttatgc
    11401 ctatctggat attggtttta actcgttaat ttccaatatt cgcaactaca aaaataaagt
    11461 tggtttactg gataactcgc ggatgaaaag cgatacccag tgggtgattg attcgatgcg
    11521 ggtaaaaacg ccgggtcatc ggacgcaaat tggttcgctc tccggtggta atcagcaaaa
    11581 ggtgattatt ggtcgctggc tactaacgca accagaaata ttaatgctcg atgaaccgac
    11641 gcgcggtatt gatgtcgggg cgaagtttga aatttatcag ttaattgccg aactggcgaa
    11701 gaaaggcaag gggattatta ttatctcctc tgaaatgcct gagttgttag ggataacaga
    11761 ccgtattctg gtcatgagca atggtctcgt ttccggaatt gtcgatacaa aaacaacaac
    11821 gcaaaacgaa attctgcgtc ttgcgtcttt gcacctttaa gattaggggc ttcccatgag
    11881 tgcgttaaat aagaaaagtt ttcttactta cctgaaagag ggcggtattt acgtcgttct
    11941 tttagttttg ctggcgatta ttattttcca ggacccaaca tttttaagtc tgttgaactt
    12001 aagtaatatt ctcacccagt catcggtgcg tattattatc gcgctcggtg tggcagggtt
    12061 aattgtcacc caggggaccg atctttctgc tggtcgtcag gtagggctgg cggcagtggt
    12121 ggctgcgaca ttattgcagt ccatggataa cgccaacaaa gtgttcccgg aaatggcgac
    12181 gatgccgatt gcgctggtta ttctgattgt ctgtgccatt ggtgcggtga tcggtttgat
    12241 caacggtctg attatcgctt atctcaacgt gacgccgttc attaccacgc tcggcacgat
    12301 gatcatcgtc tatggaatca actcgctcta ttacgacttt gtcggggcgt cgccaatttc
    12361 tggttttgac agtggcttct ctacctttgc tcagggcttt gtcgcgctgg ggagtttccg
    12421 tctctcttac atcaccttct acgcgttgat tgcggtggcg ttcgtctggg tgttgtggaa
    12481 caaaacccgc ttcggtaaga acatttttgc cattggcggt aacccggaag cggcaaaagt
    12541 atctggtgtc aacgtcggcc tgaacctgct gatgatctac gcgttgtctg gcgtgttcta
    12601 tgcctttggc gggatgttag aagccggacg tatcggctct gccaccaaca acctcggctt
    12661 tatgtatgag ctggatgcta tcgcggcgtg cgtggtaggc ggtgtatcgt tcagcggcgg
    12721 tgtggggacg gtgattggcg tggtgaccgg ggtaattatt tttaccgtca tcaactatgg
    12781 cctgacgtat atcggcgtaa acccatactg gcagtacatc atcaaagggg cgattattat
    12841 cttcgccgta gcgctggatt cactgaaata cgcgcgtaag aagtgatgtg attgtggata
    12901 ttgccggatg cgctgcatcc ggcaatcggt gccaaatgcc ggatgcgtct ctggttttac
    12961 aacgttaccg ggtgttcttt ctcgcctttc ttaaacttca cttccccgag ctcaatacaa
    13021 cccaccgggc agacgtgacc acaaagcaga caacccacac atttctcggt attacaatgc
    13081 ggtgtgcggg ttttctcgct ccattccatc gcctggtgac cgccgtcgta acaggaaata
    13141 taacagcgtc cacagccaac acatttatca agattgatac ggggatagac aatataactg
    13201 cggtctaaat cttccgccgg gacaatattg ttattcgcca gacctaccat ctcctgcagc
    13261 gaatcaaatc cctgatcggc gagataatgc gacaacccgc ttgccatatc ttccactatc
    13321 cgatacccgt actgcatgat gccggtggtc acctgtaacg ttgctgcgcc gagcaataaa
    13381 aactcagccg catcctccca ggtttcaatg ccgccgatac cgctgattgg gaaatcgcgc
    13441 agttctggat gggtgcgcat ctgctgaatg aagcgcaggg cgatcggttt taccgcttta
    13501 ccggaatatc cggaaatact cgattttccg ttaacgatcg gcataccgat tttctgatta
    13561 agatcgatat tggtgatgga tttaacggtg ttaatcgccg caatgccatc tgcgccgccg
    13621 cgcttcgccg ccagcgccac ttcgcacata tcaccgatat tcggcgtcat cttcgccagc
    13681 attggcagcg tggaaccccg tttcactgcc cgacaatatt tttctaccag ctccgggctt
    13741 tgcccgacat cgctacccat cgcatgagaa gtcatttgcg gacaggagaa gttacactcg
    13801 atcatatccg cgccagcttc ttgcaccagg cgcgccagct cctcccattg ctgctcattt
    13861 tcccccatga tcgaagcgat caatactttg tccgggtaat cttccttcag ctgacgcagg
    13921 gcggccagat tctcttccaa cggatgttca gcaatctgct ccatattttt gaagccgata
    13981 aaaccggtat cttctttcac cagatgatca aaacgcggcg agacttcgtt ggcgataaaa
    14041 aagccgatcg ttttaaacac cacgccgccc caacctgtgt cgtaggcttt ggcacacatc
    14101 tcatagcagt tgcctacagg cgaagaagag aggcagaacg ggttgggaaa cttcacgccg
    14161 caaaaagtaa tcgaaagatc tttcgttaac atgagcaagc tccctctaaa tagtgatgaa
    14221 tcgcctcggc ggcttctttc ccggttttca cggcatagac cacggttttg tcaccctcaa
    14281 caatatcgcc agcagcaaag acttgcggat cgcgggtctg ataattttgt gttttgatgg
    14341 tgttacgctg cggctctaac tccgcaaagg catccagtct ggcatgctga ccgacggcga
    14401 gaataatttt atctgccgcc atcgtcagtt cgcccggtaa ccgtacatgc ttaaacgtga
    14461 ctttattgcc ttccacggct actggcgtga atccatcgat gatcgaaacg cccagctccc
    14521 gggcgctggc aaactctttt tcgcttgccg gaaactcatc taactcttca cgcgctacgc
    14581 aggttaccgc ctgacagccg agaactttca gcgtgctggc tacgtccatc gcaacatcac
    14641 cgccgccgat aattaatgcg ctttgtggaa tgctgatatc gccttgcgcc tgtcgtgcac
    14701 gttgcaggaa gtcgacggca atctcaacgt cgctatgctc aaacagcgat aacccggaac
    14761 cgctcgataa ccccacggtg accagtaccg cgcggttttc tgctttcagc tgctcaaggg
    14821 tgagtgtgtt accgacttcg ttgttgcact taatggtcac gcccattttt tcaatacggg
    14881 cgatctctgc atccagcact gattgcggta accggaattg cggaataccg ttacgcaacc
    14941 agccaccggg gtgcgcttct ttctcataaa tcgtgacgtc ataaccctgg tttgtcagtg
    15001 ttacactggc ctgcaatccg gcaggacctg cgccaataat cgcgactttg ccgagcgttt
    15061 tagtaccggg ctgataaatt tccatcccgg tttgttgttc aaaatcagta acaaaacgct
    15121 gtaagcgacc gatatcaatg ggagtatcaa caccggcacg ggtacaaccg ctttgacata
    15181 atttctccgt cgggcacact ctggcacaaa cggcaccgag ggcattattt tcgcgaattg
    15241 tctcggcagc gcctttaaaa ttacgaaagt agattgagcg aataaatttc cccggatcgg
    15301 tctgtgccgg gcaagcctga ctacagggag cgtcatgaca taataaacag cgagaggctt
    15361 ctttaatcgc cagtaaaggc gtaaaagccg gagtgagttc atccagataa ttttgttgcg
    15421 gcatgtgatc ttccttaatg ctatgagtaa ttaaggcgaa cgctaaatat actcgtcata
    15481 cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct
    15541 cctggggact cactcacttg tcgccttcct gcaactcgaa ttatttagag tataaatatt
    15601 gcgtgctgat attaaggcta tgcgcgtggg gaaatttata ctgtgaaagg attcacaaat
    15661 agctgcggtt cagaatgtta tatccgcagc ttattgagtg aagtaataat tattctcaat
    15721 aattaacaga aaagagaaaa tattaacgat agaaaataag ataaacaatc atgccgatag
    15781 ataaaattcc gaagccgata tatgccacca tataagcgaa aaagagtccg ggattttttg
    15841 ctcgccagac atacaccggt tccggtgcat ttttaacccg tttatcaatc gcgcctgcgc
    15901 gccagccttt ccatgcttcg cggaaacaaa atatcggaat aaggaaaaaa acggcaacga
    15961 caaaaaactg ctgtacatcc ataactactt tccttgtttc ttttgtaatt caagtaactg
    16021 ttcgggtgtg acggcgggat acccctgcgc atcttccggc acctggtgca tagccggaat
    16081 agggaccagc ggccctaaaa aacgcggttc acgtttaaaa atataaaggt cagccagcgc
    16141 accgaaacgg gcggcaaatt cgcgaatacg tactgacagc atatctttcg gtgacggtac
    16201 ggcataacac tgagcctgaa tccccatatg cagcgcaata aataatgccc gctcacagtg
    16261 gaaacgttgg gtgataataa tgaaatcatt agtatcgaaa actttgcgtg tacgcacgat
    16321 ggaatccagc gtacgaaagc ctgcgtaatc gagaacaata tctgatgggt cgacaccagc
    16381 agcgattaaa tctttgcgca tggtcatcgg ctcattataa ctttgcaatg cgttatcgcc
    16441 gctcagtaat agataattta ccttaccgct gttataggca ttaatcgctc cttgaatgcg
    16501 gtagcgataa tactgattaa ttacgccagt acgataatat tttgctgttc cgagcaccac
    16561 accgacctgg cggtagggga gatcctgcaa ttcgtcgtag atataaggcg cggttttcca
    16621 gctcatccag cgatcgaggc cgagcacagt caacagcagc aagccgatca ggactaacag
    16681 gctgaggaac acgcgcttta acatgcagga gactcgatct aaggaaaatt tccatcaggc
    16741 tactttaccc gctggttttg cgcaagaaac ggcaaactgt ttgccgggaa tttcgacacc
    16801 aggagagggc tggcaagccg gacaaggcgt tcacgccgca tccggcacca ggcttaagcg
    16861 agaagcactc ggtcgatact gtgacagccg agagctttca gcgttgcgga ggtggcatcc
    16921 cactgaatca acggcgcatc ggctttttct gccagaaccg cgcgctggat atccgggtaa
    16981 tcataaccct tgagattcaa cagaattaac gctccctgca acggtggcag agtcgggttg
    17041 aatgcggcgt tttcagcgta gctgccactg aaaatacgac cgtctttaca ttccagcgcg
    17101 acaccgcttg gcgacttact gtaaggcatg tgcgaacggt ttgccgctgc aatcgccgcc
    17161 tgagaaagcg catcaccagt cagcgcatag ccgtgatcct gctcgtccat cagcagcgtt
    17221 ttaatctcca gatctttcgg cccaaaggca tccggcagat agtcacgcag cgcgtgtgcc
    17281 tcgcggcccg gcagatgaat acgcagatcc agaccgctgt tcagttcatt cataaactga
    17341 cggcagtgac cacaaggcgt gtagttaacg gtgatggctg caagcgcttt ttcaccactc
    17401 aaccaggcgt ggctgatcgc gctttgttcg gcatgaacgg tttgctgcat tgtcgcacca
    17461 ataaattcca tattggcacc gaaataccag gttccgctca caccgcgcgc aattgcgcca
    17521 acattaaaat tcgacaatgg cgtacgcgca caggccgccg ccagcggaag tagtgcgaat
    17581 gccagcgcgt cttcgtccag ccccgttgcg ctcttcagcg atgagacttg ctccccggtc
    17641 aacaaagcgg ggaagtactt gtctgccaga ataggttcca gtgcagattg caagttatcc
    17701 gcaagttggg caaaagcggt ttgaaaacgt ggatgcatgg cgttgcctca taatcatgta
    17761 atgggataac agtttacgaa cccgttatgg ctttatatgt gatctgaatc tcattaatta
    17821 tgcaacgcgt gaaacttaca tcaaaaatgc gcgacgcatc gcaaatttta gcccattact
    17881 gccagaataa tcgggaaaag gaacggtgcg atcagcgaag taattatccc gcataacacc
    17941 agcgctagcg aactaaatgc accttcctga taatccagct cggcgcagcg cgccgtaccg
    18001 agggcgtgcg aggcagtccc cattgccaga ccgcgcgcag ctttggtacg aatacgcatc
    18061 gcattaagca atgtatggcc aaataccgcg ccgaggatgc cgacgaaaat cacgcaaact
    18121 gcgctgattg ccggaatacc gccaatactg ccgccaaccg ccattgcaat aggcgtggtg
    18181 actgatttcg gcaggattga cgcggcgatt tccggtgaag cgcccatcaa caatgccacg
    18241 gaagtccccg tcaccatcgc aaccacgctg ccgataaaac agatggtgat gatcgatttc
    18301 cagcgcgcgc ggatctggtg tagctgctca tataaaggat aggccagcgc gacgaccgcc
    18361 ggttgcagca gatcgtttag cacttcgcta cctttaaagt agctgtcgta agagatgccg
    18421 gtcagcatta aaaaaggaat gatcaccacc attgctacca gcaacgggtt aagcagggga
    18481 aacttatacc gtgccgccag tttgcgggcg gcaaaaaaga caatcaacgt cagcggtaat
    18541 gaccaccaga tattcgccat cattcttctg atcctttctg acctacgact ttacgttcac
    18601 cgtgtaccag ttgcgaactc cagctcacca ccagaaaaac caccagcgta ctgattgcac
    18661 aggaaaccac taccgggcca aactgtgcgc ggagcaaatc aaaatattgc atgacgccta
    18721 cgccaatcgg cacaaacaat agcgccatat agcgaatcag tacgtagcac cccggattga
    18781 cccatttcgc cggaagaatt tgcaaggcca gcaggacaaa caggatcagc atcccgatga
    18841 tgctgcccgg aatggttacc ggtagcaggg aagcaatgaa aatgcctgca tacaggcagg
    18901 cataaatcag gacgaaagcg cgtaaatatt gccagataat gttcagtgtc ttactcataa
    18961 tcaacacccc agatgagatg gattcatcat acaattaatt cgtaaaacgt gctaccgatc
    19021 acattaagaa atcagcgctt attttttcgc tccccggtat tacgcaatga gcagcgaact
    19081 caggcataca tcaagcatag agagggattg cagtttgcag caatccctct ttgcagacct
    19141 ttaatccgtt gaccataggc gctaacatag cgcctcattt ttttgcgggt gatgatatgc
    19201 gtgtgttact ggcaccgatg gagggagtgc ttgactctct ggtgcgtgaa ttgctgaccg
    19261 aagttaacga ctacgatctg tgcatcaccg agtttgtccg cgtggtggat caactgctgc
    19321 cggtaaaagt ctttcatcgc atttgccctg agctacaaaa cgccagccgg acaccatctg
    19381 gtacgctggt gcgcgtgcag ttgttaggtc agttcccaca atggctggca gagaacgccg
    19441 cccgtgcggt ggagttaggt tcctggggcg tggatctcaa ttgcggctgc ccgtcgaaaa
    19501 cggtcaacgg tagcggtggt ggggcgacgt tactcaaaga tcctgaactc atctaccagg
    19561 gtgcaaaagc gatgcgtgaa gctgtaccgg cgcatttgcc cgtcagcgtg aaagtgcgtc
    19621 tgggctggga cagcggtgag aagaaatttg aaatcgccga tgcggttcaa caggctggtg
    19681 ctacggagct ggtggtgcat ggacggacaa aagagcaggg ttaccgcgcg gagcatattg
    19741 actggcaggc gattggcgag attcgccagc ggctgaatat tccggtgatt gccaacggtg
    19801 aaatctggga ctggcagagt gcgcaacaat gtatggcgat cagcggctgc gacgcagtga
    19861 tgattggtcg cggggcgctc aatattccca acctgagccg ggtggtaaaa tataacgaac
    19921 cgcgaatgcc gtggccggag gtggttgctt tgctgcaaaa atatacccgt ctggaaaagc
    19981 agggcgatac cgggttatat cacgttgcgc ggattaaaca gtggttgagt tatttgcgta
    20041 aagaatacga tgaagcaacg gaattatttc agcatgttcg ggtgttgaat aattcccctg
    20101 atattgcaag ggctattcag gcaattgata tcgagaaact ctaaaaaacc atagtgatgt
    20161 tttttgacaa tgtgttagct ctttatcaat aggccgcacg gttgtttggt ggcgtcaaaa
    20221 t
//
LOCUS       DAZGAN020000085        20029 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGAN020000085 DAZGAN020000000
VERSION     DAZGAN020000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.085"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1046
                     /locus_tag="ACHKYO_RS17095"
                     /old_locus_tag="pgaptmp_003419"
     CDS             <1..1046
                     /locus_tag="ACHKYO_RS17095"
                     /old_locus_tag="pgaptmp_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4430204.1"
                     /translation="NVAFEAFKAGAFDLRMENDAKNWATRYTGKNFDKKYIIKDEQKN
                     ESAQDTRWLAFNIQRPVFSDRRVREAITLAFDFEWMNKALFYNAWSRTNSYFQNTEYA
                     ARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPVSKGDGYDRDNLLKADKLLNEAGWVL
                     KGQQRVNATTGQPLSFELLLPASSNSQWVLPFQHSLQRLGINMDIRKVDNSQITNRMR
                     SRDYDMMPRVWRAMPWPSSDLQISWSSEYINSTYNAPGVQSPVIDSLINQIIAAQGNK
                     EKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAWWDKFSQPAVRPVYSLGIDTWWYDVN
                     KAAKLPSASKQGE"
     gene            1047..2141
                     /locus_tag="ACHKYO_RS17100"
                     /old_locus_tag="pgaptmp_003420"
     CDS             1047..2141
                     /locus_tag="ACHKYO_RS17100"
                     /old_locus_tag="pgaptmp_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HGU4430205.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            2141..3166
                     /locus_tag="ACHKYO_RS17105"
                     /old_locus_tag="pgaptmp_003421"
     CDS             2141..3166
                     /locus_tag="ACHKYO_RS17105"
                     /old_locus_tag="pgaptmp_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4430206.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            3168..4757
                     /gene="yejF"
                     /locus_tag="ACHKYO_RS17110"
                     /old_locus_tag="pgaptmp_003422"
     CDS             3168..4757
                     /gene="yejF"
                     /locus_tag="ACHKYO_RS17110"
                     /old_locus_tag="pgaptmp_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HGU4430207.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(4761..5113)
                     /locus_tag="ACHKYO_RS17115"
                     /old_locus_tag="pgaptmp_003423"
                     /pseudo
     CDS             complement(4761..5113)
                     /locus_tag="ACHKYO_RS17115"
                     /old_locus_tag="pgaptmp_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
     gene            complement(5446..6636)
                     /gene="bcr"
                     /locus_tag="ACHKYO_RS17120"
                     /old_locus_tag="pgaptmp_003424"
     CDS             complement(5446..6636)
                     /gene="bcr"
                     /locus_tag="ACHKYO_RS17120"
                     /old_locus_tag="pgaptmp_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HGU4430208.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(6664..7359)
                     /gene="rsuA"
                     /locus_tag="ACHKYO_RS17125"
                     /old_locus_tag="pgaptmp_003425"
     CDS             complement(6664..7359)
                     /gene="rsuA"
                     /locus_tag="ACHKYO_RS17125"
                     /old_locus_tag="pgaptmp_003425"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HGU4430209.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            7508..9268
                     /locus_tag="ACHKYO_RS17130"
                     /old_locus_tag="pgaptmp_003426"
     CDS             7508..9268
                     /locus_tag="ACHKYO_RS17130"
                     /old_locus_tag="pgaptmp_003426"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU4430210.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHEHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            9393..9677
                     /gene="rplY"
                     /locus_tag="ACHKYO_RS17135"
                     /old_locus_tag="pgaptmp_003427"
     CDS             9393..9677
                     /gene="rplY"
                     /locus_tag="ACHKYO_RS17135"
                     /old_locus_tag="pgaptmp_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HGU4430211.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(9816..10823)
                     /gene="yejK"
                     /locus_tag="ACHKYO_RS17140"
                     /old_locus_tag="pgaptmp_003428"
     CDS             complement(9816..10823)
                     /gene="yejK"
                     /locus_tag="ACHKYO_RS17140"
                     /old_locus_tag="pgaptmp_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HGU4430212.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            11005..11232
                     /locus_tag="ACHKYO_RS17145"
                     /old_locus_tag="pgaptmp_003429"
     CDS             11005..11232
                     /locus_tag="ACHKYO_RS17145"
                     /old_locus_tag="pgaptmp_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HGU4430213.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            11252..13012
                     /gene="yejM"
                     /locus_tag="ACHKYO_RS17150"
                     /old_locus_tag="pgaptmp_003430"
     CDS             11252..13012
                     /gene="yejM"
                     /locus_tag="ACHKYO_RS17150"
                     /old_locus_tag="pgaptmp_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HGU4430214.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            13087..13163
                     /locus_tag="ACHKYO_RS17155"
                     /old_locus_tag="pgaptmp_003431"
     tRNA            13087..13163
                     /locus_tag="ACHKYO_RS17155"
                     /old_locus_tag="pgaptmp_003431"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13121..13123,aa:Pro,seq:ggg)
     gene            13382..14623
                     /locus_tag="ACHKYO_RS17160"
                     /old_locus_tag="pgaptmp_003432"
     CDS             13382..14623
                     /locus_tag="ACHKYO_RS17160"
                     /old_locus_tag="pgaptmp_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HGU4430215.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLIKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALIRAGTDPQDHERQKRETEARR
                     QGNTFENVATAWYQVKISQNLAPNTIKDIWRSLEKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRINEVMDYAANSGMIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPVEASSARWEEIDMEAQRWTIPAGRMK
                     MRRDHVIPLCDQAMAVLEAMKPISHHRDYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            15130..15336
                     /locus_tag="ACHKYO_RS17165"
                     /old_locus_tag="pgaptmp_003433"
     CDS             15130..15336
                     /locus_tag="ACHKYO_RS17165"
                     /old_locus_tag="pgaptmp_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4430216.1"
                     /translation="MEAIKKVIFRQEVKKLIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEEDVIAWMKEREQEARGTTA"
     gene            complement(15458..15661)
                     /locus_tag="ACHKYO_RS17170"
                     /old_locus_tag="pgaptmp_003434"
     CDS             complement(15458..15661)
                     /locus_tag="ACHKYO_RS17170"
                     /old_locus_tag="pgaptmp_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430217.1"
                     /translation="MTNKESTNTPSQKTNREHENMALEHESERFAPCAFVLDEFLKKY
                     SRSERMKMAAQYGPNKQDNCPPA"
     gene            complement(15719..15898)
                     /locus_tag="ACHKYO_RS17175"
                     /old_locus_tag="pgaptmp_003435"
     CDS             complement(15719..15898)
                     /locus_tag="ACHKYO_RS17175"
                     /old_locus_tag="pgaptmp_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4430218.1"
                     /translation="MPTGTTNNKSQQKTARVPLDVLSEMNLVKEENETDAQFIVTAMR
                     GEIKRRQRRKAKESE"
     gene            16083..16283
                     /locus_tag="ACHKYO_RS17180"
                     /old_locus_tag="pgaptmp_003436"
     CDS             16083..16283
                     /locus_tag="ACHKYO_RS17180"
                     /old_locus_tag="pgaptmp_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4430219.1"
                     /translation="MVAVNHTPRLAHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            16273..16533
                     /locus_tag="ACHKYO_RS17185"
                     /old_locus_tag="pgaptmp_003437"
     CDS             16273..16533
                     /locus_tag="ACHKYO_RS17185"
                     /old_locus_tag="pgaptmp_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430220.1"
                     /translation="MHNKTTPDAAAAALTTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQLSADMALNEAKAILIADCENGGGLCVMIVLIP"
     gene            16506..16727
                     /locus_tag="ACHKYO_RS17190"
                     /old_locus_tag="pgaptmp_003438"
     CDS             16506..16727
                     /locus_tag="ACHKYO_RS17190"
                     /old_locus_tag="pgaptmp_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430221.1"
                     /translation="MRDDRFNSLKQEFSGVSDDAADALSSISEIMRVAFFFLCTDEHR
                     DTGLNILDIAANYADFVTEVILRKATDGD"
     gene            16729..16962
                     /locus_tag="ACHKYO_RS17195"
                     /old_locus_tag="pgaptmp_003439"
     CDS             16729..16962
                     /locus_tag="ACHKYO_RS17195"
                     /old_locus_tag="pgaptmp_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430222.1"
                     /translation="MRDIYHETTDRVFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IVTALITLLEPVIMELQEIDQLHDRYNEQHTGE"
     gene            16968..17267
                     /locus_tag="ACHKYO_RS17200"
                     /old_locus_tag="pgaptmp_003440"
     CDS             16968..17267
                     /locus_tag="ACHKYO_RS17200"
                     /old_locus_tag="pgaptmp_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4430223.1"
                     /translation="MKFKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQAVPRNVPTSREKARANIQKMKNMINGFRGKK"
     gene            17264..19018
                     /locus_tag="ACHKYO_RS17205"
                     /old_locus_tag="pgaptmp_003441"
     CDS             17264..19018
                     /locus_tag="ACHKYO_RS17205"
                     /old_locus_tag="pgaptmp_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HGU4430224.1"
                     /translation="MKLAPNVKQQPRGIKHKETEVIIFAGSDAWSHAKQWQEHDARMA
                     GDNEPPVWLGEQQLSELDKLQIVPEGRKSVRIFRAGYLAPVMIKAIGQKLAAAGVQDA
                     NFYPDGMHGQKVENWREYLASERQNLSDGLVIELPVKQKAQLSQMADSERAQLLAGRF
                     DGVCVHPESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVI
                     AEPMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNAPN
                     FHKWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLA
                     GKQNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKY
                     EKRFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAE
                     IPVIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLM
                     MGGGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKV
                     KGVTQTNAITTDECDAFL"
     gene            19307..19585
                     /locus_tag="ACHKYO_RS17210"
                     /old_locus_tag="pgaptmp_003442"
     CDS             19307..19585
                     /locus_tag="ACHKYO_RS17210"
                     /old_locus_tag="pgaptmp_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430225.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKNVEVES"
     gene            19582..20004
                     /locus_tag="ACHKYO_RS17215"
                     /old_locus_tag="pgaptmp_003443"
     CDS             19582..20004
                     /locus_tag="ACHKYO_RS17215"
                     /old_locus_tag="pgaptmp_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4430226.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLTCSQADDGT
                     ATMWLSVLAFGRQADALAKHRKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
BASE COUNT         5165 a         5017 c         5117 g         4730 t
ORIGIN      
        1 ataatgtcgc ctttgaagcg tttaaagcag gtgcctttga tttgcgtatg gaaaacgacg
       61 ctaaaaactg ggccacgcgt tataccggta aaaatttcga taaaaaatac atcatcaaag
      121 atgagcaaaa gaacgaatca gcccaggata cgcgctggct ggcgtttaat atccaacgtc
      181 cggtattcag cgatcgccgg gtccgggaag caatcaccct cgcctttgac tttgaatgga
      241 tgaacaaggc gttgttttac aatgcctgga gtcgcacgaa cagttatttt cagaataccg
      301 aatacgcggc cagaaattac cccgacgccg cggagctggt gcttctggca ccaatgaaaa
      361 aagatctacc gccagaagtc ttcacacaaa tctaccagcc gccggtatct aaaggcgatg
      421 gctacgatcg tgacaacctg ttaaaagccg acaaacttct caacgaagcg ggctgggtgc
      481 tgaagggtca gcaacgcgtt aatgccacaa cgggtcagcc actcagcttt gaattattgc
      541 ttcccgcaag cagcaatagt cagtgggtat tgccgttcca gcacagcctg caacggctgg
      601 gtatcaacat ggacattcgc aaggtggata actcgcaaat caccaaccgc atgcgcagtc
      661 gcgactatga catgatgccg cgcgtatggc gggcgatgcc gtggcccagt tccgatttac
      721 agatttcctg gtcatcggaa tatatcaatt ccacttataa tgcccccggc gtgcaaagtc
      781 cggttatcga ctcgctgatc aatcaaatta ttgccgcgca gggaaataaa gaaaaattac
      841 tgccgttagg gcgagcactg gatcgcgtat taacgtggaa ttattacatg ctgccaatgt
      901 ggtacatggc ggaagaccgt ctcgcctggt gggataaatt ctcccaaccc gctgtacgcc
      961 ctgtttacag cctgggtatc gatacctggt ggtatgacgt taataaagcg gccaaactgc
     1021 cgtccgccag caaacaggga gagtagatgg gcgcttacct gattcgccgt ctgttgctgg
     1081 tgatcccaac attatgggcg attatcacca tcaacttttt catcgtgcaa attgcgcctg
     1141 gcggtccggt cgaccaggcc atcgccgcca ttgagtttgg taatgccgga gtattacccg
     1201 gcgcaggcgg tgaaggtgtt cgtgccagcc atgcgcaaac gggtgtcggc aatatcagcg
     1261 acagtaatta ccgtggcgga cgcggattag atccagaagt gatcgctgag atcactcatc
     1321 gctacggttt tgataagccg atccacgaac gttacttcaa aatgctctgg gactacatcc
     1381 gctttgattt tggcgatagc ctgtttcgca gcgcctcggt gctgacgctg attaaagaca
     1441 gtctgccggt ttccatcacc ctcggattgt ggagcacgct gattatctat ctggtgtcga
     1501 ttccgttagg cattcgcaaa gctgtttata atgggagccg ctttgacgtc tggagtagcg
     1561 catttatcat catcggctac gccattccgg cctttttgtt tgccatcctg ctgattgtct
     1621 tcttcgcggg cggcagctat ttcgacctgt tccctctacg cggcctggtt tccgctaact
     1681 ttgattcgct gccgtggtat cagaaaatca ccgattatct gtggcatatc acgctgccgg
     1741 tgctggcgac agtgattggt ggctttgcgg cgctgaccat gctgaccaaa aactcattcc
     1801 ttgatgaagt gcgcaagcaa tacgtggtga ccgcgcgtgc gaaaggggta agtgaaaaaa
     1861 atattctctg gaaacatgtg ttccgcaacg ccatgctgct ggtgattgcc ggttttccgg
     1921 cgacgtttat cagcatgttt tttaccggct cgctgctgat tgaagtgatg ttttcactca
     1981 atggccttgg cttactgggc tacgaagcga ccgtctcgcg cgattatcct gtaatgtttg
     2041 gtaccttgta tattttcacc ctgattggcc tgctgctgaa tattgtcagt gatatcagct
     2101 atacgctggt cgatccgcgt atagattttg agggacgtta atgtcgcgac tcagccccgt
     2161 caatcaggcc cgttgggcgc gttttcgcca taaccgtcgc ggctactggt cgttatggat
     2221 tttcctcgtt ttgtttggtt tgagtttgtg ttctgaactt atcgccaacg ataaaccgtt
     2281 gctggtacgt tatgacggca gttggtattt cccgttgttg aaaaactaca gcgaaagcga
     2341 ttttggcggc ccgctggcaa gtcaggctga ttatcaggac ccgtggctga aacaacggct
     2401 ggaaaataac ggctgggtac tgtgggcacc gattcgcttt ggtgctacca gtatcaactt
     2461 tgctaccgat aagcccttcc cttctccacc ctcccggcaa aactggctgg gaacggatgc
     2521 caacggcggc gatgtgctgg cacgcattct ctatggcacg cggatctcgg ttctgtttgg
     2581 cctgatgctg actctctgtt ccagcgtgat gggcgtgctg gcgggggcgc tacaaggcta
     2641 ttacggcggt aaagtcgatc tctggggaca acgctttatt gaagtatggt cggggatgcc
     2701 gacgctgttt ttgattattt tactttccag cgtcgtacag cctaacttct ggtggctgct
     2761 ggcaattact gtcttgtttg gctggatgag tctggtcggc gtggtgcggg cggagttttt
     2821 acgtactcgt aatttcgact acattcgtgc ggcacaggcg cttggcgtca gcgatcgcag
     2881 tatcatcctg cgtcatatgt tgcctaatgc catggtcgcg accctcacct ttttaccgtt
     2941 tattttatgt agttcgataa ccaccctgac ctcgctcgat ttcctcggct tcggtctgcc
     3001 gctcggttca ccgtcactcg gtgaactgct gttacaaggg aaaaataacc ttcaggctcc
     3061 gtggcttggg atcaccgcct tcttgtcggt ggcgatatta ttgtctttgc tgatctttat
     3121 tggtgaagcc gtccgcgacg catttgatcc taataaggcg gtgtagcatg acgcaaactc
     3181 tgttagcgat tgaagatttg tcggtgggtt ttcgccatca gcaaaccgta cgtacagtag
     3241 tcaatgatgt ttcactacag attgaggctg gcgaaacgct ggcgctggtg ggtgagtcag
     3301 gttcaggcaa aagcgttacc gcgctgtcaa ttttacgcct gctcccttcc ccgccggttg
     3361 aatatctctc cggcgatatt cgttttcgtg gcgaatcgct gcttcatgcc agcgatcaaa
     3421 cgttgcgcgg tgtacgcggt aataagatcg ccatgatttt tcaagagccg atggtgtcgt
     3481 taaatccatt gcataccctg gaaaaacagc tttatgaagt gctttcactc caccgcggga
     3541 tgcgtcggga agcggctcga ggcgaaattc ttaactgcct tgatcgcgtt ggtatccgcc
     3601 aggcggcaaa acggctgaca gattatccgc atcagctctc cggcggcgaa cgccagcggg
     3661 tgatgattgc gatggcgctg ttaacgcgac cggaattatt aattgccgat gaaccgacca
     3721 ccgcactgga cgtctctgtc caggcgcaga ttttacagct gttgcgcgaa ctgcaaggcg
     3781 agctgaatat gggcatgctg tttattactc ataacctcag cattgtcaga aaactggccc
     3841 accgcgtggc ggtaatgcaa aacggtcgct gtgtcgagca aaataacgcc gctacgctat
     3901 ttgcctcacc cactcatcct tacacacaaa agctactcaa cagtgaaccg tcaggcgacc
     3961 cggtgccgtt gccagaacct gcctcaacgt tgctggatgt tgaacagctt caggttgcct
     4021 tccccattcg caaagggatt ttgaagcgca ttgtggatca taatgtggtg gtgaaaaaca
     4081 tcagttttac gctacgagcg ggtgaaacac tgggtttagt gggcgagtcc ggttccggga
     4141 aaagtacgac gggactggcg ctgctgcgac tgattaattc tcagggcagc atcatctttg
     4201 acggtcagcc actgcaaaat ttaaatcgcc gccagctgtt acctattcgt catcgcattc
     4261 aggtggtatt tcaggatcca aactcctcgc tcaacccacg actcaacgtt ttgcagatta
     4321 ttgaggaagg cttacgggtt caccagccga cgctttctgc cgcacaacgc gaacaacaag
     4381 tgatagccgt gatgcatgaa gtgggattag atcctgaaac acgccaccgt tatccggcgg
     4441 agttctctgg tggtcagcga caacgtattg cgattgccag ggcattaatt cttaagccct
     4501 cgctgatcat acttgatgaa ccgacatcat cactcgacaa aacggtacag gcgcaaatat
     4561 tgacgctatt gaaatcattg caacaaaagc atcaactggc ctatttgttt atcagccacg
     4621 atttgcacgt tgtccgcgcg ttatgtcatc aggttatcgt actgcgacaa ggggaagtag
     4681 tggaacaagg accgtgcgcg cgcgtgtttg ccacaccgca gcaggagtat acgcgtcagc
     4741 tactggcgtt gagctgacgc ttaaaaagga ttgtagtttg aaaagggttc ggcaattgcc
     4801 acaccaaaat ttttcaatcg gcatgttgca gcaaactcgt cctggcgatt tacaaacagg
     4861 cacggctcac cttcacactc cagcaccgaa catggaactt cgatgtcgct taacgcctgg
     4921 cttagtttat acatcaccga ccatgcatta tcgccatccg ggctaagcaa tttaagcgcc
     4981 accaggctgt tgtcaccgca cggtccattt tgcggaatcg gttcaacctt cgaaccttcg
     5041 aacctgcgaa accagcagac cagcgatagt tctgcggcag tcgatggacg attgagagtt
     5101 gtaatgaagt cacttatttt ccccggaagt acattcactt cacaatttgt ttacatcaat
     5161 tttaacacat catcaacaat ccgtctttat acagatcgta aagattgatg cctcgcctgc
     5221 gtggcctcta tggctctgat ttaagtaata tctgcgggcg tggtcacgtt tgcggggcta
     5281 tatgtacccg tttctgcgat ctaactcaac ctttttaact acaatgatgt gactttttac
     5341 ataaattgat tttacataaa ataaacatat atcggggaaa tgatagattt gtggttgaca
     5401 tgcatcaaca aaggaagcct tttaccttcc tcgttgtgca atagatcacc gttttttcgg
     5461 ccgactggcg tacagacaga agagaatgga gctggttgcg cagaatgcaa ttgaccaaat
     5521 catcggccat gcagagttaa aggtcgcaag agaaagcaat gcgccaacaa ttgcccctat
     5581 gccaaaacgg aaggttcctg ccagcgaaga tgccgttccc gccatatggg gaaactcatc
     5641 aagaatgacc gccatcgcat tggatgacac catcgacacg cagcccacaa acgccgcaac
     5701 gccaaccacc agcgaccaga atcccagccc cagcagcgca ctgatgacca tccacgctgc
     5761 cataataaat tgtatccaca accccgagcg gaacatattt aacgcgccaa tgcggcgaac
     5821 gaagcggctg ttaaagatgg tcatcacgaa caggaaaaca atgtttagtg caaagtaata
     5881 accaaagttt tccggcgcga cgtggttaat ttcaatgtag acaaacggtc cggcgctcag
     5941 gaacgagaac attccggcaa agctgaaacc gctggccagc atataactca ggacacgctt
     6001 atgacggaac agcgccgcaa agttaccaat agtggtacga atgtgaaatg gctgacgacg
     6061 ctccggtggt aaggtttctt taatcaggaa gaaaatcatt gccgaagcca gaatcgccgc
     6121 taatgccagg atccagaaga tgtaatgcca gctcagccac accagcaccc aaccgccgac
     6181 aatcggtgcc atcaacggtg caatagtggt caccagcatg acaaacgaca tcatccgcga
     6241 gaactcttct ttcgggtaaa tatcgcgcat caaagcgttg ataaccacgc tggccgcagc
     6301 cgccgccagc ccgtggaaga aacgcatcac aatcagctga tcgatggtgt ttgccaacgc
     6361 acacgccacc gcggcggcgg caaacaccag cgtaccgccg agcaccaccg gcttacgccc
     6421 gaagctgtct gccatcggcc cgtagattaa ctgccccaac gcaaagccca gaatataagt
     6481 actgagggtc atctgcgtac tgcccgcagg tacgccaaac tgcgctgaaa ttaccggtag
     6541 cgcgggcaga tacatatcaa tcgacagcgg catcaacatg gccagcaggc caaggataaa
     6601 aacaatagca aacgacgaat gctgtcgggt ggtcacaacg ggctcctgaa agtcatggaa
     6661 aagttagacg acgctggcaa tttcttcttc agttaacgga cgatattcac cgggggctaa
     6721 atcagcatcc agcgtaatac cgccaatacg ttcacgatgc agctcaacca cgtggttacc
     6781 cacggcggcg aacatgcgtt tcacctgatg ataacgccct tcgctgatgg tcagacgaac
     6841 ctgcgttggg gtaatcactt ccagcaccgc aggcttagtg agatcttttt cgttatgcag
     6901 ctgcacgcct ttagcaaatt gctctgccgt atcgtcagct acaggtgatt ccagtgtcac
     6961 cagataggtc ttctcgcaat gatggcgcgg agaagtaatg cggtgcgacc actgaccatc
     7021 atcagtcatc agcaccagac cggtggtatc gatatccaga cgtcctgcgg cgtgcagttt
     7081 ccaggctacc ggttcatcca gaaaatagag caccgttggg tgatcagggt cgtccgtgga
     7141 gcaaacatag ccctgaggct tattgagcat gaagtaacgt ggaccatgtt gctgcgccag
     7201 cgggttgcca tcgtaagcga catcatgttc aggaagcagt ttgaacgctg cattacgaac
     7261 aatttcgcca tcgacggtga cacgattgcc gcggatttca cgcccggcaa tagcacggct
     7321 aacgccgagt tgctgtgcga taaatttatc aagtcgcatg tgtgtgattt tgcctgtaaa
     7381 aacggaggtc gggcattgac ccgaaaatct gaactgttgt ctgcccagta tagcggtcta
     7441 ataacatccc tcaagggaaa aagatccatg gcatactatt agcagaataa tctacctacg
     7501 cgagaccatg atttttacac tccgcccata tcagcaagaa gccgtggatg ccacgctcaa
     7561 ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cccaccggcg caggtaaaag
     7621 cctggtgatt gccgaactgg cacggctggc gcgtggtcgt gttctggtgc tggcacacgt
     7681 caaagaactg gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg aagccgatat
     7741 ttttgccgct gggttaaagc gcaaagagag ccacggtaaa gtggtatttg gcagcgtgca
     7801 gtcggtcgct cgtaatcttg atgcctttca gggtgaattt tcgctgttga ttgtcgatga
     7861 atgccaccgt attggtgacg atgaagagag ccagtatcag caaatcctca ctcacctgac
     7921 taaagtgaat ccccacttac gcctgctggg gctgactgcc acgccttttc gattgggcaa
     7981 aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga aagccctttt
     8041 ccgtgactgc atttatgagc tgccgctgcg ttatatgatt aaacacggct atctgacgcc
     8101 gccagaacga ctggatatgc cagtagtgca atacgatttc agccgcttgc aggcacagag
     8161 taacgggctg ttcagcgaag ctgatctcaa ccgtgagctg aaaaaacagc aacgcattac
     8221 gccacatatc atcagccaga tcatggagtt cgccgaaaag cgcaaagggg taatgatttt
     8281 cgccgccacc gttgaacacg caaaagagat tgtgggatta ctgcctgccg aagatgctgc
     8341 actgattact ggcgacaccc ccggcgctga gcgcgatgtg ttaattgaag attttaaagc
     8401 ccagcgtttt cgctatctgg tcaacgtcgc agtactgacc accggatttg acgccccgca
     8461 cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc aacaaattgt
     8521 cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt ttaattcttg attatgcggg
     8581 taatcctcac gatctctacg cgccggaagt tggtacaccg aaaggcaaaa gtgacaacgt
     8641 tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg ggaaaacgac
     8701 cgccgacggg acattgattg aacactttgg tcgccgctgt cagggatggt ttgaagatga
     8761 cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc cgcaatgtaa
     8821 cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt gacacggtac tggttgatcc
     8881 ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg ctggtattac gctgtagcgg
     8941 catgtctttg cagcatgagc acgacgagaa aggcgaatgg ttgaaaatca cctattacga
     9001 tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc agcgaactgc
     9061 cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatccctc tgcgctggat
     9121 caccgccgcc gatatcctcg cccagcaagc cttattgcga cacccggatt ttgtcgttgc
     9181 ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg ttcgattacg aaggtcgttt
     9241 tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata aagcgattga
     9301 gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacctgt tgctgggtcg
     9361 cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca gaagtacgta
     9421 aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc ccggcaatca
     9481 tctacggtgg caaagaagca ccgctggcta tcgagctgga tcacgacaaa gtcatgaaca
     9541 tgcaagctaa agctgaattc tacagcgaag ttctgaccat cgttgttgac ggtaaagaaa
     9601 tcaaagttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg cagcacatcg
     9661 acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg cggggttttt
     9721 tgtatctgca gattatgcct gattacggta ttgctatttt ttgcaggcca gataaggcgt
     9781 tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga cgtccggcgc
     9841 tgcaattggt cacgcaaatt cggcggcgtg cctttaatgg tcagggtatc ggttgccgga
     9901 tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa gccaccgccg
     9961 ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa gctctcttcc
    10021 agttcgtagc ctttttcagc cgcaaactcc gtgaaactca cttcgctgac gcctgccagc
    10081 tctttcgaca gcgactccag ttcgatctct tcgcctgcct gcagctgctc attacagtag
    10141 ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc ttcggcagtg
    10201 aaatcatcaa ccgcttgcaa cagcccacgg ttttgcgctt tcgcgtttaa cccttcgctg
    10261 gccccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc tttcaggaaa
    10321 gtgagatagc gggtggactc tggattggtt tcccattcgg ttaaatcgat acgcgcaaca
    10381 atatccgcat gattgatatc aagataatgg gttggattga tgtcgagatt ttcgttaacg
    10441 cgcatactgc tcaggttgct cagaactgcc accagcagat actccaccgc cagataacgg
    10501 tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa ttcgtcacgc
    10561 aggcgcccgg ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg acgctgtaag
    10621 cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt atttttggcg
    10681 ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg ttccagcaat
    10741 gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg gtgcagagca
    10801 atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg tattcaacca
    10861 ccgccggtta agcgacgcaa taaaagatgg cggtcatttt acagaaggca gcgtaacgag
    10921 aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata tgttgccctt
    10981 tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtga tgaacaggtt
    11041 gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc gaccgacctt
    11101 tccctgatgg tgttaggcaa catggtcact aaccttatca ataccagcat tgccccggcc
    11161 caacgccagg caattgccaa ctcttttgcc cgcgccttac agtcctctat caacgaagac
    11221 aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc gctaccgtga
    11281 aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata ttctgctttc
    11341 gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgacaacgc ttgctggtcg
    11401 catttattcc tacgtaagca ttatcggcca tttcagcttc ctggtgttcg ccacctactt
    11461 gctgatcctc ttcccgctga cctttatcgt cggctcccag aggctgatga ggtttttgtc
    11521 cgtcattctg gcaacggcgg gaatgacgct attactgatc gatagcgaag tctttactcg
    11581 tttccatctc catcttaatc ccatcgtctg gcaactggtt atcaacccag acgaaaatga
    11641 gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat tgcttgaact
    11701 ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc ggcgcttcgc
    11761 gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg tgtatatctg
    11821 ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc cgctttcgta
    11881 cccgatgacg gcgcgacgtt ttcttgagaa gcatggtctg cttgatgcgc aggagtatca
    11941 acgccgtctt attgagcaag gtaatccaga cgccgtttcc gttcagtatc cgttaagcga
    12001 actgcgctat cgcgatatgg gcaccgggca gaatgtgctg ttgattactg tcgatggcct
    12061 gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg agcaaaatat
    12121 ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct ttggcctgtt
    12181 ctatggcatc tcgccgagct atatggacgg cattctgtcg acccgtaccc ctgcggcgtt
    12241 aattactgcg cttaatcagc aaggctatca gctgggatta ttctcatcag atggctttac
    12301 cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg tacgcaccca
    12361 atccgacgag cagaccgcca cgcagtggat caactggctg ggccgctacg cacaagaaga
    12421 taaccgctgg ttctcgtggg tctctttcaa tggcactaac attgacgaca gcaatcagca
    12481 ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga tcaaccgcgt
    12541 gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta tcactgccgg
    12601 tcggggtatt ccgctgagcg aagacgaaga aacctttgac tggtcccacg gtcatctgca
    12661 ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg ctctgactga
    12721 tcataccgat ctgatgacga cgctgatgca acgcctgcta catgtcagca cacctgccag
    12781 cgaatattcg caaggtcagg atttgttcaa ccctcaacgc cgtcattact gggttactgc
    12841 agcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc tgaacaataa
    12901 cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa aaccacagtt
    12961 aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt atcgctaact gattaattat
    13021 aaatcagtta gcaaaatatc ttacttgcaa tcggtgtgga aaacggtagt attagcagcc
    13081 acgagtcggc acgtagcgca gcctggtagc gcaccgtcat ggggtgtcgg gggtcggagg
    13141 ttcaaatcct ctcgtgccga ccaaatcgcc caccggatac caccggaaat caccgaataa
    13201 gacagggata gcccgccaat tacttgattg gcgggttttt ttatggtttt tagtcaccga
    13261 acatcaccgg atttataagc tatcagccgg attttttagt tatacgttta gttatacggg
    13321 ctgacgtata actaaagccg tataactaaa accctgtttt ttcgtttgga atcataagca
    13381 tatggcgaga aaaacacccc cattaaccac agtacagatc aaagcagcca gaccagcaga
    13441 aaaggaatac accctacagg acggcggagg gctttttctc ctgatcaaac cgtccggatc
    13501 aaaactctgg agattttcct actaccgacc atcggacaaa aaaagaatat tgctgagttt
    13561 tggatcgctt gatgatgttt ccctggctga tgccagaaaa cgccgtagcg agtacagggc
    13621 gttaattcgt gccggaactg acccgcagga ccacgagagg caaaaaagag agacagaggc
    13681 ccgaagacaa gggaacacgt tcgaaaatgt ggcaacagca tggtaccagg tgaaaatcag
    13741 ccagaatctg gcccccaata cgattaaaga catctggcga tcgctggaaa aatatgtatt
    13801 cccgttcatc ggcaacacgc caatagacac cctgaccgct cgcaggttcg ttgaggtgct
    13861 tacccccatc aaggaacgtg gcaacctaga aacgctcaag cgagttttac agcgtataaa
    13921 tgaggtaatg gattacgccg ccaatagtgg gatgattgat gccaatccgg ctatgaatgt
    13981 gcgtaaggcg ttcccctccc ctgtaaaaaa acatatgcca acaatccgcc ccgaacagct
    14041 gccggagctt atgcaggctt tatcagtatc ggcaacagaa cggcagacca gattactgat
    14101 tgaatggcag ttactgaccg taacccgtcc cgttgaagca tcgtcggcac gctgggaaga
    14161 aatcgacatg gaagcgcaac gctggaccat acccgccgga cgcatgaaga tgcgccgcga
    14221 ccacgttatc ccgctttgtg accaggctat ggcagtgctg gaggctatga aaccaatcag
    14281 ccaccaccgc gattatgttt tcccgagtct gaaagaccca cagcagccga tgaacagcca
    14341 gaccgctaac gcagcattac gacgcatggg attcgctggc gtgctggtgt ctcatggatt
    14401 acgcgccata ttcagcacag cagcgaacga agaaggcttt gagccggacg taatagaggc
    14461 cgcacttgcc cacgtcgaca ccaacgaagt cagacgggca tacaaccgga gcaactacat
    14521 agaaaaacgc atcgtgctta tgcgctggtg gggcgaattt gtcgaggctg cggcgacggg
    14581 cgtaaccctc gccagtggta aaaggggtat ccgagccgtt tagctgtaca gaaaaccagt
    14641 aaaaactacg aaaaccattt aaaaccgtcg tataattgca tcacatttaa cgacgaggcc
    14701 ttgaaaaatg aaactgttaa gatgcaaaca aatttcagat gcaattgcga cgggctgcaa
    14761 ctggccctga tggttcagca tgaattttgg tcaacctacg atccggagga cagaacgacg
    14821 gccccatcaa aaaaacaggt agtagatttc ctggtatccc gtggggcttc cagaaatctg
    14881 gctgtaagta ttgataaggt tgtacgtccg gcatccatga agaccggagg caggccaaaa
    14941 aaatggcggt aacaatcctg aaagcggcag aaatgccgct ttttttataa atccctttca
    15001 aatcatcaat ataaaaaatg gtgtataccg tttaaaaacg gtgagtactg tttttccctg
    15061 catccgatga tttaccgtat ttctcaccgg aatacaccgg aatacaccgg attaatgagg
    15121 taaatcacga tggaagcaat caaaaaagtc atctttcgcc aggaagtaaa aaaacttatc
    15181 cacattaagg cagacagcac gctgcaaagc atgatcaacg ccggagaatt cccgcagggt
    15241 tttcgtgtcg gtttgcgccg tcgcggatgg tatgaggaag acgtaatcgc atggatgaaa
    15301 gaacgcgaac aagaagcgcg cggaacgacc gcttaacata tatagggcaa atgatgaaca
    15361 taaaaaaagc ggccccgaaa tggagccacc tttctgaaca attaacccgc tgcgccgtat
    15421 gtgtcaacaa tcataagccc gggcatgata accgcgatca ggctggtggg caattatctt
    15481 gcttgttggg gccgtattgt gctgccattt tcatccgttc agaacgtgaa tattttttta
    15541 gaaactcatc aaggacaaaa gcgcatggtg cgaatctttc tgattcatgc tcgagcgcca
    15601 tattttcgtg ctctctgttc gttttttgcg atggtgtatt ggttgattct ttgttggtca
    15661 ttgtgtgttc ctgtaaagca atgcgccgga gtacctcaca ccacggcgct gatagtgatt
    15721 attctgattc tttggccttg cgacgctggc ggcgtttgat ttcgccgcgc atggcagtaa
    15781 cgataaattg tgcgtctgtt tcgttttctt cttttaccaa attcatttcg cttaaaacgt
    15841 ctagaggtac tctagcagtt ttttgttgtg acttgttgtt ggttgtacct gttggcatct
    15901 ctgttctcct tactgttagg tggtgttcag tatacggaga aaaaaaaata aaaaaagact
    15961 tgaagtgcaa gtcacctaaa agtaacctag atttcaaggt gacttgcacc aaaaaacaac
    16021 gccccacagt gctggaacac atgcagggcg tctaaccaca aacactatag agaggtaaat
    16081 ttatggttgc cgtaaatcat accccacgac tagcacacac acaaacggcc tttgtgtggc
    16141 gttttctggc actgagtgcc ggagaatctc aaatcatcca cgtaaccgcc tggacggaac
    16201 gcgaagcgcg tagccgttgc ccgtccggtt gtgttgctgt atttgcagcc cgtattcgcc
    16261 agggagaaac ctatgcacaa taaaaccaca ccggacgcag cagccgccgc gctcactacg
    16321 ctgatgcacg cgcttattga tatttctgtt attgctgaca gggcgcataa gcacgccacc
    16381 agtgaaacag aatatgcagg ggctttcgtt cctcattcgc tggcggttat gcaacttagt
    16441 gctgatatgg cgctgaatga ggccaaagct atcctgattg ctgattgtga aaatgggggg
    16501 gggttatgcg tgatgatcgt tttaattccc tgaaacaaga attttccggc gtttctgatg
    16561 atgcggctga tgcgctttcg tcaatatctg aaattatgcg ggtggctttt ttctttcttt
    16621 gcactgatga gcacagagat acagggctaa atattcttga tattgccgct aactatgctg
    16681 atttcgtgac agaggttatt ttaagaaaag cgacggacgg ggattaatat gcgtgatatt
    16741 taccacgaaa caacagatcg cgtatttctt gcactttctc acagtgaaaa tatgatggaa
    16801 atattgcgca tatggcttga aacacttggc gacaatgaac gcgacaaaca aaaatcaaga
    16861 attgtcacgg cattaataac gcttcttgag cctgtaataa tggaactaca agaaatagac
    16921 caattgcacg acagatataa cgaacagcac accggagaat aaaaataatg aaatttaaat
    16981 attctggctt aactgccagt ggcaacactc accctaaatt tacgcgcggt gatatttacc
    17041 gcgaccagta cggcggcacg gtaatgatta agggcgtggc gggacggtgc gtaacttacc
    17101 gccgtgaagg ttacgaatat gattgcgtga tgcctgttta tcagttccgg cgtgattttt
    17161 ctcttgtgca ggcagtaccg cgtaacgtgc ccaccagcag ggagaaagca cgcgccaata
    17221 ttcagaagat gaaaaacatg attaacggat tcaggggcaa aaaatgaaac tggcaccgaa
    17281 cgtaaaacag cagccacgcg gcataaaaca caaagaaaca gaagtcatta tttttgcggg
    17341 tagtgatgcc tggtcacacg cgaaacaatg gcaggaacat gacgcgcgta tggccggaga
    17401 taatgagcct cctgtgtggc ttggggagca gcagttatca gaactggata agctgcaaat
    17461 tgtgccggaa ggcagaaaat ccgtgcgcat attcagggcc ggatatcttg cgccagtaat
    17521 gataaaggcg attggtcaga agctggcggc ggcaggcgta caggatgcaa atttttaccc
    17581 tgatggtatg cacggtcaga aggtggagaa ctggcgcgaa tatctggcca gtgagcgcca
    17641 gaatctttct gatgggctgg ttattgagct tcctgtaaag caaaaggcgc aactttcgca
    17701 gatggcggac agtgagcgcg ctcaacttct tgccggacga tttgatggcg tttgcgtgca
    17761 tcctgaaagt gaaatcgttc acgtatggcg cggcggggta tggtgtccgg tcagcacaat
    17821 ggaacttagc cgcgaaatgg tggcgatcta ttcagagcac agggccactt tcagcaagcg
    17881 cgtaatcaat aacgccgtgg aagcgttaaa agttattgcc gaaccaatgg gcgagccgtc
    17941 cggcgatttg ctgccgtttg ccaatggtgc gcttgacctg aaaacggggg aattttcccc
    18001 gcacacgccg gagaactgga tcaccacgca caacggtatt gagtacacgc caccagcacc
    18061 aggggagaac atccgcgata acgcgccaaa ctttcataaa tggcttgagc acgcagccgg
    18121 aaaagacccg cgcaagatga tgcggatatg tgccgcgctg tacatgatta tggcgaaccg
    18181 gtacgactgg cagatgttta ttgaggccac cggagacggc gggagcggta aaagtacatt
    18241 cacacacata gccagccttc tggcagggaa acagaacacg gtaagcgctg aaatgacatc
    18301 gcttgatgat gctggtggac gtgcgcaggt tgtcgggagt cgccttatcg tcctggctga
    18361 ccagccgaaa tatacaggcg aaggaacggg catcaagaaa atcacgggcg gtgaccccgt
    18421 ggaaattaac ccgaaatatg aaaagcgttt tacggcggta atcagggcgg tggtgctggc
    18481 gaccaacaac aacccgatga tattcaccga acgggccgga ggtgtggcac gtcgtcgcgt
    18541 gattttccgt ttcgacaata tcgtcagtga ggccgaaaaa gacagggagc taccggaaaa
    18601 gattgcggct gaaatccctg tcattatccg ccgattgctg gcgaacttta ccgactctga
    18661 gaaagcacgg gctttactac tggaacagcg tgacggtgat gaagctctgg caataaagca
    18721 acagacggat ccggttattg agttttgcca gttcctgaat tttctggagg aagcacgcgg
    18781 cctgatgatg ggcggcggtg gcgattcagt gaagtacacg accagaaaca gcctttaccg
    18841 cgtctatctg gcgtttatgg cgtacgcagg caggagcaaa ccgctaaacg taaatgactt
    18901 tggcaaggct atgaagccag ccgcgaaagt ttacggacat gaatatatta cgcggaaagt
    18961 taagggagta acgcagacta acgcaataac aacagacgag tgcgacgcgt ttttataatt
    19021 ttttgtaatt gctgtctacc ctgtctacct gagtaaagaa agatacattt aattcagtgc
    19081 attaagttag gtagatagcc tttttttact gtctacccac tatctaccct ctctacctga
    19141 ttttacctga atcagacagg gaggtagaca cggggtagat agcggataaa agcactctac
    19201 cccgctgaaa gccgcgccat tactggcatg agggccacta aggtagataa ggtagacaag
    19261 gggaggcaca actcaaaact ttttaaacga gggggtaaaa ataaatatgc acacttcagg
    19321 gaaatttaat aaatcactca aaaaacgcag agacagaaca gaaccgaaat atcgcgcgtt
    19381 agacatgaca gagcacgctt taaaggtggc aatcagaacg atagaccgcc acgcggggga
    19441 aggatacgcg aaagcacatc ccgaactgat aagcgcattc atgaccacga cggcggcaaa
    19501 ttttgccacg ctgacagagc gggagattgc ggaagctgaa caggtgacaa ccatcaacgt
    19561 taaaaacgta gaggtggaat catgacagca cagatagcgg cttacgggcg gctggtggct
    19621 gacccacagt taaagaccac cagcaagggc acacaaatga cgatggcgag tatggcggtt
    19681 cccctgacat gcagccaggc tgatgacgga acggcgacga tgtggttatc cgtcctggcg
    19741 tttggcagac aggccgacgc actggcaaaa caccgcaaag gcgagctggt gagcgtggcg
    19801 ggtaacatgc aggtaagcca gtggacaggc cagaacggcg aaacgcggca gggctggcag
    19861 gttatcgcag acagcgtaat cagtgcgaga acggcgcgac cgggcggtaa aaaaggccag
    19921 caggggcagg ctactgacgc actgaacagg gcaaaacaac aggcggggaa tgatgatccg
    19981 tacggggata acataccgtt ttaagcaacg agtgacagaa gccggagca
//
LOCUS       DAZGAN020000086        19663 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGAN020000086 DAZGAN020000000
VERSION     DAZGAN020000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.086"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..140)
                     /locus_tag="ACHKYO_RS17220"
                     /old_locus_tag="pgaptmp_003444"
     CDS             complement(<1..140)
                     /locus_tag="ACHKYO_RS17220"
                     /old_locus_tag="pgaptmp_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4430227.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NV"
     gene            complement(532..1575)
                     /locus_tag="ACHKYO_RS17225"
                     /old_locus_tag="pgaptmp_003445"
     CDS             complement(532..1575)
                     /locus_tag="ACHKYO_RS17225"
                     /old_locus_tag="pgaptmp_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4430228.1"
                     /translation="MTGKLRFEVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAHLAYVFLEQNAPRKALNAALKGLAVGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLRRHQDAIMLIEKILDYNPEDNHGARWLLGPEL
                     LRTGAHEQARHILQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYVWPWILPF
                     SAAGMKHTGIQYQ"
     gene            complement(1831..3096)
                     /locus_tag="ACHKYO_RS17230"
                     /old_locus_tag="pgaptmp_003446"
     CDS             complement(1831..3096)
                     /locus_tag="ACHKYO_RS17230"
                     /old_locus_tag="pgaptmp_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4430229.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRINS
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAERKPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TKEVCGHGFRAMACSTLIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREECISPFEYAKVNNPLKR"
     gene            complement(3294..3369)
                     /locus_tag="ACHKYO_RS17235"
                     /old_locus_tag="pgaptmp_003447"
     tRNA            complement(3294..3369)
                     /locus_tag="ACHKYO_RS17235"
                     /old_locus_tag="pgaptmp_003447"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3334..3336),aa:Phe,seq:gaa)
     gene            complement(3475..4182)
                     /gene="yqgA"
                     /locus_tag="ACHKYO_RS17240"
                     /old_locus_tag="pgaptmp_003448"
     CDS             complement(3475..4182)
                     /gene="yqgA"
                     /locus_tag="ACHKYO_RS17240"
                     /old_locus_tag="pgaptmp_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HGU4430230.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            4335..4418
                     /gene="yqgH"
                     /locus_tag="ACHKYO_RS17245"
                     /old_locus_tag="pgaptmp_003449"
     CDS             4335..4418
                     /gene="yqgH"
                     /locus_tag="ACHKYO_RS17245"
                     /old_locus_tag="pgaptmp_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HGU4430231.1"
                     /translation="MHAISLHSLAYRHFARNVSLSMLLLMR"
     gene            4580..6715
                     /gene="speC"
                     /locus_tag="ACHKYO_RS17250"
                     /old_locus_tag="pgaptmp_003450"
     CDS             4580..6715
                     /gene="speC"
                     /locus_tag="ACHKYO_RS17250"
                     /old_locus_tag="pgaptmp_003450"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HGU4430232.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(6765..8021)
                     /gene="nupG"
                     /locus_tag="ACHKYO_RS17255"
                     /old_locus_tag="pgaptmp_003451"
     CDS             complement(6765..8021)
                     /gene="nupG"
                     /locus_tag="ACHKYO_RS17255"
                     /old_locus_tag="pgaptmp_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HGU4430233.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(8223..9302)
                     /gene="mltC"
                     /locus_tag="ACHKYO_RS17260"
                     /old_locus_tag="pgaptmp_003452"
     CDS             complement(8223..9302)
                     /gene="mltC"
                     /locus_tag="ACHKYO_RS17260"
                     /old_locus_tag="pgaptmp_003452"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HGU4430234.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRNNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(9367..9642)
                     /gene="yggX"
                     /locus_tag="ACHKYO_RS17265"
                     /old_locus_tag="pgaptmp_003453"
     CDS             complement(9367..9642)
                     /gene="yggX"
                     /locus_tag="ACHKYO_RS17265"
                     /old_locus_tag="pgaptmp_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HGU4430235.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(9670..10722)
                     /gene="mutY"
                     /locus_tag="ACHKYO_RS17270"
                     /old_locus_tag="pgaptmp_003454"
     CDS             complement(9670..10722)
                     /gene="mutY"
                     /locus_tag="ACHKYO_RS17270"
                     /old_locus_tag="pgaptmp_003454"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGU4430236.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            10883..11602
                     /gene="trmB"
                     /locus_tag="ACHKYO_RS17275"
                     /old_locus_tag="pgaptmp_003455"
     CDS             10883..11602
                     /gene="trmB"
                     /locus_tag="ACHKYO_RS17275"
                     /old_locus_tag="pgaptmp_003455"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGU4430237.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            11602..11928
                     /gene="yggL"
                     /locus_tag="ACHKYO_RS17280"
                     /old_locus_tag="pgaptmp_003456"
     CDS             11602..11928
                     /gene="yggL"
                     /locus_tag="ACHKYO_RS17280"
                     /old_locus_tag="pgaptmp_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HGU4430238.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            12112..12831
                     /gene="yggN"
                     /locus_tag="ACHKYO_RS17285"
                     /old_locus_tag="pgaptmp_003457"
     CDS             12112..12831
                     /gene="yggN"
                     /locus_tag="ACHKYO_RS17285"
                     /old_locus_tag="pgaptmp_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HGU4430239.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            13007..14053
                     /gene="ansB"
                     /locus_tag="ACHKYO_RS17290"
                     /old_locus_tag="pgaptmp_003458"
     CDS             13007..14053
                     /gene="ansB"
                     /locus_tag="ACHKYO_RS17290"
                     /old_locus_tag="pgaptmp_003458"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HGU4430240.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            14170..15177
                     /gene="yggM"
                     /locus_tag="ACHKYO_RS17295"
                     /old_locus_tag="pgaptmp_003459"
     CDS             14170..15177
                     /gene="yggM"
                     /locus_tag="ACHKYO_RS17295"
                     /old_locus_tag="pgaptmp_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HGU4430241.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(15332..16468)
                     /gene="hemW"
                     /locus_tag="ACHKYO_RS17300"
                     /old_locus_tag="pgaptmp_003460"
     CDS             complement(15332..16468)
                     /gene="hemW"
                     /locus_tag="ACHKYO_RS17300"
                     /old_locus_tag="pgaptmp_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGU4430242.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(16461..17054)
                     /gene="rdgB"
                     /locus_tag="ACHKYO_RS17305"
                     /old_locus_tag="pgaptmp_003461"
     CDS             complement(16461..17054)
                     /gene="rdgB"
                     /locus_tag="ACHKYO_RS17305"
                     /old_locus_tag="pgaptmp_003461"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HGU4430243.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDNQRQARFHCVLVYLRHAEDPTPLVCHGSWPGMITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(17062..17352)
                     /gene="yggU"
                     /locus_tag="ACHKYO_RS17310"
                     /old_locus_tag="pgaptmp_003462"
     CDS             complement(17062..17352)
                     /gene="yggU"
                     /locus_tag="ACHKYO_RS17310"
                     /old_locus_tag="pgaptmp_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HGU4430244.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(17349..17915)
                     /gene="yggT"
                     /locus_tag="ACHKYO_RS17315"
                     /old_locus_tag="pgaptmp_003463"
     CDS             complement(17349..17915)
                     /gene="yggT"
                     /locus_tag="ACHKYO_RS17315"
                     /old_locus_tag="pgaptmp_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HGU4430245.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(17933..18637)
                     /gene="yggS"
                     /locus_tag="ACHKYO_RS17320"
                     /old_locus_tag="pgaptmp_003464"
     CDS             complement(17933..18637)
                     /gene="yggS"
                     /locus_tag="ACHKYO_RS17320"
                     /old_locus_tag="pgaptmp_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HGU4430246.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            18655..19635
                     /gene="yggR"
                     /locus_tag="ACHKYO_RS17325"
                     /old_locus_tag="pgaptmp_003465"
     CDS             18655..19635
                     /gene="yggR"
                     /locus_tag="ACHKYO_RS17325"
                     /old_locus_tag="pgaptmp_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HGU4430247.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
BASE COUNT         4950 a         4797 c         5093 g         4823 t
ORIGIN      
        1 ataacattat cattgacgcc attttcacgt gcaatctgtg caacacaagc tccaggttgt
       61 gatgccagtt ccaccatacg aagtttgaat tcattcgaat agtttttacg aggttctttt
      121 cgccagtcct gtaattccat acttagatgt ccgtctatat cagatgggcg tctaagttac
      181 caattctcgt ctgatggcta catacggcgg tcagtttacg cttacgatta tcttgctaat
      241 taccagactg gagtaaccag accaaccgaa aacaccgggg aggtgattac ccgctggcag
      301 aggaaatccg gtttttttga tgtcagccat aacgtgtttg acaataaagc attatgaaag
      361 ggtgttaatg agtccatggg ataattcata aaaataattt actgtgttat ccttttcaaa
      421 agaaatattc agaagataac ccctggctga caaccagggg aaggggacat taacagtgtt
      481 attattggtg gataaattat tacttgtgtg caggatacac taatgcaata tctactgata
      541 ctgtatgcca gtatgtttca tgcctgccgc tgaaaacggc aatatccagg gccagacata
      601 ttcaccattc atatttctgc atttctgtac aattttctca gaaagcgtct catcaattct
      661 ttcacgcaac ttctcctgct gtcttaaaat actttcgcat atttcgaaat catcttcctg
      721 catcagcatt tcggcgcatt taatgatttc tgcccgttca tgcagcacag aggaatgatt
      781 atagagccag tttacaaaca gcaatgcttc cggatattgc ccccataacg ggtggtatgt
      841 ggcataataa tcttcggcag tatccggacc gcctgaaaag ttatgccata cagcaagtgg
      901 aaatggatgc agatttccgc acagtatttc tgcgatgtaa gtatttgccg caaagccgcg
      961 acgaaatgca gttgcagctt ttaccagttc cccattaaga aaatgcagaa ggccgagttc
     1021 ataccagtaa ggtgaaaatt catctgcatg ttcctgcagt atatggcgtg cctgttcatg
     1081 agcgccagtg cgtaacagtt ccggtcccag tagccagcgt gcaccatgat tatcttcggg
     1141 attgtaatcc agtatttttt caatgagcat gatggcatcc tgatgacgcc gtaaatgtgc
     1201 atttgccagg attgccgcat agagggcacg aagaaaaggt cggttgtcag gatgtatcca
     1261 gatgattctg ccactgaaac cttcagggat aagacgattg ccaactgcca gccctttaag
     1321 ggcagcattc agcgccttac gcggggcatt ctgctccaga aatacgtatg ccaagtgagc
     1381 gtgtacatca ataaagtcat tctcctgtcg ggcaaggcgc ctgagtttgt tgatgtaact
     1441 tgtttcgctg atttcatccg catcgtatgc atcaatcagt tcttcaaact catccagcaa
     1501 cgagccaaac caggtttccg gaaatatgaa acagccctgg ttatcgttca cttcaaagcg
     1561 taatttgcca gtcatattct gaacctgtaa aaaaggatgg accataatct gcaggctata
     1621 aaaattgtga tgcctggcat cggtgtcctt ttattgtccg ggattaacgt tgcccatgat
     1681 aatacagtga atcctgttct gtggtaagac gttgagttat tgaatgagaa cctcagcctg
     1741 gctgatggtt ccgccggcat aaaaaagaaa ttgattgagg cgggtgctct tccccttaag
     1801 aggaagagca tgtgtgtaaa tggtgttcag tcaccttttc agtggattgt taacttttgc
     1861 atattcaaac ggactgatac actcctccct gtttgcatcc agaaaatctg cccaccattg
     1921 cagcataagc cgacgttctt ccagatgttc tgctttatga atatacgcgg cacgtactcc
     1981 gttgcgctcc tgatgactca tctgacgttc aaccgcatct cttgaccata agccggattc
     2041 aatcaatgta ctgcacgcca tcgcccggaa tccatggcca cagacttcct tggttgtgtc
     2101 ataccccatc actctgaggg ccttatttac ggtattttca ctgattggtt tacgcgggtc
     2161 atgtgctccc gtaaagatca gaccattttc accagcccat gtctgaagtt ctgtcagtat
     2221 ggctacagcc tgtttgctga gtggcacaag atgtggtgtt cgcatcttgg agccccggtg
     2281 tgagaatttt acccctggca gaggtttgcg ttcggctgga atagtccaca tggcattgtc
     2341 aatatcaatt tcagaccagc gggcgtatct gagttcactg gaacgaataa aaaccagtaa
     2401 attcagcata acggcaagcc gggtgacagg ctgaccttta taactgttaa tacgcgacag
     2461 aagttcaggc agacgtgaaa gatcaagagc ggggcgatgc tggcgtttta ccgtagtcaa
     2521 tgcgccagcc atatccagag caggattata tctgatgatg ccagactgta cggcataacg
     2581 cattacggca gatattcttt gctgcagacg actggcgatt tcataaattt gtttagcttc
     2641 tgcagcacga acagggataa gcagatccgg ggtattgagt gttgtgatat cccggttgcc
     2701 gataaagggg aaaacgtgtg tttcgaggct tttgagtact ttatcgctgt gtgattgtgc
     2761 ccattgttta ttactggcat gccagcgtcg tgcaatatgt tcaaaagaga gctgtaaatc
     2821 agggactact tttttagctc ttttctgttc gcaagggtca atcccctccg aaatcagttt
     2881 tcgggcctca tcccgttttg tacgagcttc ggagagagaa acctccgggt atacaccaag
     2941 tgccagagtc ttctccttac ccagaatacg ataacggaga cgccagtaac gggaaccatt
     3001 aggatgtacc agcaggaaca tgccagcccc gtcagtgagt ttataagcct tgtcagtagg
     3061 ctttgctgcc cggatttttg cgtcagtcag tgccatagtt cagccttctg aatgcgttaa
     3121 agagggttca aattctgtcg aactggggta tgcccacaat tatacccaca caatgaggtt
     3181 gatgtggggg gaatctggtt gagttcggta gaattgattt ggaggcagga cgcttaaatc
     3241 gtggcgtcct ggaatgaaaa acggacctcc gtggaggtcc gttgatatga atttggtgcc
     3301 cggactcgga atcgaaccaa ggacacgggg attttcaatc ccctgctcta ccgactgagc
     3361 tatccgggca acggggcgca ttaaacctga ttcgcctcgt ctcgtcaatc aaatttcttc
     3421 aatttactgc agactgcaca atctatcatc actttgccgc tattgcacgc attgtcaggc
     3481 aaaccaggcg gtccaggcgg cggaaagcgg cattgccaga aggagtgctg gaagcatgtt
     3541 gaccaccggg aacattttaa tgccacagat gcgtaatccg gttgccagca gcaataaacc
     3601 gcctacggcg ctgaagtctg ccatcatcga cggtgtggtc agcggtaata tcagcgcggc
     3661 agcccacgcc agcgttaact ggatgatcag taatgggata ctaattaccg acaccgcaat
     3721 acccagcgag caggcgaaga tcatcgccgt aaagaaatca agaaatgact tggcgattaa
     3781 aatactcgga tcgccggtca tcccttcgtt catcgccccg aagatcccgg tgccgctggc
     3841 gcaaaacagg acaataatcg cgacataatt ctgaataaaa gattcatgcg ctggcttctt
     3901 acgtgagtga cgaaacagat tttgtgcttt ggcgactgct gtattgacac ctttctccag
     3961 caaacaaatt tcgccaatta gagcgccaag tagcgtagct aaaaccatcg ctggaaggtt
     4021 ggcgcatttc acaaccagta aaataccaat ccccagcgat gccagaccaa aaatagatgt
     4081 catggagacg cggatacgtt ccggtaagcg ttggctgagc agtgcgccga gaacgccacc
     4141 cagtaagact gcactagcgt tgataaaagg gccgatgacc acaagagttc ctgttagctg
     4201 tattgtttga ttttcttatt atgaactttt tgccttgttg atggcttgtc tggtcgcaaa
     4261 gtcctggctt gcacgcttta gcgaaaggtg ccatgattgc gcgaattttc tcctctctgt
     4321 acggagtttg cccgatgcac gccatctcct tacattctct cgcttatcgc catttcgcgc
     4381 gaaacgtttc cctttctatg ttactgctca tgcggtgaag ttaacgcacg ctcactgcag
     4441 gacaacagta aaatcagagc gtttctgctt ttactgatgt ctggcggtcg gagctggtga
     4501 ccagtttgac ccatatctca tggggcaggg ttttccacct tgtccggtat tcttacttcc
     4561 ccgaaacggg tttgcgctta tgaaatcaat gaatattgcc gccagtagtg aactggtatc
     4621 ccgactttct tctcatcgtc gcgtggtggc gttgggagat actgatttta cggacgtcgc
     4681 ggcagtcgtc attaccgctg cggatagtcg cagtggcatt cttgcgttgc ttaagcgcac
     4741 cggttttcat ctaccggtgt ttttgtattc cgaacatgct gttgaattac ctgcgggcgt
     4801 tacggcggta atcaacggca acgagcagca gtggctggag ctggaatccg cagcctgtca
     4861 gtatgaagag aatttgctgc caccgtttta tgacacgctg acgcagtacg ttgagatggg
     4921 caacagcacc tttgcttgcc ctggacatca acatggtgcg ttttttaaaa agcatcctgc
     4981 cggacgccat ttttacgatt tctttggtga gaacgtcttt cgcgccgata tgtgtaacgc
     5041 tgacgtaaaa ttgggcgatc tgcttattca tgaaggatcg gcgaaagatg cgcagaaatt
     5101 cgcagccaaa gtctttcatg ccgataaaac ctattttgtg ctgaacggca catcggcagc
     5161 gaataaagtg gtgacgaatg cgctgttaac gcgtggcgat ctggtgctct tcgaccgtaa
     5221 caaccataag tcgaatcatc acggcgcgct gattcaggcg ggggcgacgc cggtctatct
     5281 ggaagcttca cgcaacccgt ttggtttcat tggcggtatt gatgcgcact gttttaatga
     5341 agagtatctg cgccagcaaa ttcgcgacgt tgcgccagaa aaagccgagc tgccgcgccc
     5401 gtttcgcctg gcgattattc agctgggaac ctatgacgga actgtctata acgcccgtca
     5461 ggtgatcgat accgttgggc atctgtgtga ttacattctg tttgattccg cgtgggtcgg
     5521 ttacgaacaa tttatcccga tgatggcgga tagctcgccg ctgctgttag aacttaacga
     5581 aaacgatccg gggatttttg tgacccagtc ggtgcacaaa cagcaggctg gattctcaca
     5641 gacgtcgcag atccataaaa aagataacca tatccgcgga caggcgcgtt tttgcccgca
     5701 taagcggttg aataacgcct ttatgctcca tgcttctacc agccctttct atccgctgtt
     5761 tgctgcactg gatgttaacg ccaaaattca tgaaggggag agtgggcgtc ggctgtgggc
     5821 tgagtgcgtt gagttgggta ttgagtcgcg caaggctatt cttgcgcgct gtaagctgtt
     5881 ccgcccgttt atcccgcccg ttgttgatgg caaattgtgg caggattatc cgacatctgt
     5941 gttagccagc gaccgccgtt ttttcagttt tgagccgggg gcgaagtggc acggctttga
     6001 aggatatgcc gcggatcagt attttgttga tccgtgcaag ctgttactca ctacaccagg
     6061 tatcaatgcc gaaactggcg aatatagcga ctttggcgtt ccggcgacga ttctggcgca
     6121 ctatctgcgt gagaacggca ttgtgccgga gaagtgcgat ctcaactcca ttctgttctt
     6181 attaactccg gcggaaagcc acgagaagct ggcacaactg gtggcgatgc tggcgcaatt
     6241 tgaacagcat attgaggatg actcgccgct ggctgaggtg ttgccgagcg tttataacaa
     6301 atatccggtg cgctatcgcg actacaccct gcgccagttg tgtcaggaga tgcacgatct
     6361 gtatgtcagt ttcgacgtca aagacctaca aaaagcgatg ttccgccagc agagtttccc
     6421 gtcagtggtg atgaaccccc aggatgcgca tagcgcttat attcgcggtg aagtggagtt
     6481 ggtgcggatt cgtgatgccg aagggcgaat tgcggcagaa ggggcgttgc cttatccacc
     6541 tggcgtgctt tgcgtggtac ccggggaagt ctggggtggg gcggttcaac gttatttcct
     6601 tgcactggaa gaaggggtga atttgttgcc gggattttcg ccggagctgc aaggtgttta
     6661 tagcgaaacc gatgcggatg gcatgaaacg gttgtacggt tatgtgttga agtaagaata
     6721 aaaaaaacgg gtcaccttct ggcgacccgt ttttctttgc gtaattagtg gctaaccgtc
     6781 tgtgtgcctg tcgggacacg aacgtgttta tatttgaaca tcgccatgaa cgcgaaggcc
     6841 agaaccacgg agtaaccagc gaaaatcaac catacggtct gccagtcggt aatgccgttt
     6901 tgggtgtaca tctcaacaac tttaccgctc acgatgccgc cgaggataca gccgaagccg
     6961 ttagtcatca tcaggaacat cccttgtgca ctggcgcgaa ttgccgggct aacttctttt
     7021 tcgacaaaca ccgaaccaga gatgttgaag aagtcgaatg cgcaaccgta aacgatcatc
     7081 gacagtacca gcagtacagt accgaacgga gtcgggtcgc cgtaagcaaa cagcgcaaaa
     7141 cgcaggatcc acgccacaat actgatcatc attacgttct taataccgta gcggcttaag
     7201 aagaacggga tggtcagaat gaacagggtt tcagagatct gcgaaatcga catgatgatt
     7261 gacgcatgct gcacaataaa gctgctggca aacatcggat ctttgtcgaa gctgtgcagg
     7321 aaggtattac cgaacatgtt ggtaatctgc agttccgcgc ccagcagcat tgagaagata
     7381 aagaagattg ccatacgctt gtttttaaac agcgcgaatg catcgaggcc cagcagggtt
     7441 gtccagctct gattcgcttg ctgtttagca accggaatat gcggcagagt cagggtaaac
     7501 agaaccagaa tggcggaaag tgctgcgcca atatacagct gcatgtggct taattcgaag
     7561 ccagacaggc tcaccaccca cattgccatg ataaagccga tggtgcccca gatacggatt
     7621 ggcgggaagt cagtaacgat atccatcccg gcattttgca ggcgatagta agagatggtg
     7681 ttgattaacc caagcgttgg catataagca aacgagttaa tcaatatcac aaggaacatc
     7741 gcctccggtg tcgtgacctg tgccgccatg aacagcgtga tagcgccaat ggtgtggcaa
     7801 atggcatata cccatttcgc acttaaccat ttgtcggcca caatccccag cagcgcaggc
     7861 ataaagaccg cggcgatacc cagtgagcta taaactgcgc caatagaagc accgtcaaac
     7921 ttcagggtaa caaacatata ggagccgagg gtcgtcagcc aacttcccca cagacagaac
     7981 tgcagaaaag agaggatttt cagctgcagc ttaagattca tgttaatttc ctcacatcgt
     8041 gatgcggatg agtgtttgaa aaagcacatt tgaccgtgcg agatgatggg attctatcaa
     8101 cttcgcggac tggttttttg ttacctgtgg caaataattg caaacattcc caatttgcaa
     8161 aacgaaattg tgatgtggat aacatttttg cccctgagca tcgtcagggg cggttaatgg
     8221 aattatcggc ggcggtagga tttttgcgcg gtattcactt tataaagata acggcgagat
     8281 tccgcagagg gatggcgggt cgtcagcgtc tgataaacat cgcccggcgt catggtgtta
     8341 ataatattgg cagcctgaat cttatcattc gaaaagactc gcagcacgct gcctgcgccg
     8401 ccgttatagg cggtgatgac ggcataacgc cgcgatgttg ggttatcaat tccgccgaga
     8461 taaacattgt tcagcatcgc cagatacgcg gtgccggtat caatattgct ggcaggatca
     8521 aacaagaaac tgcggctcgg cgtgccggat ttcccctgcg agcggaacac atctttcccg
     8581 gcagtatgtt gtaccacctg cattaatccc agcgcatcgg aacggctgac cgcatacggg
     8641 ttaaaggaag attcggtctg cataattgcc agaatcagcg actcatcaac gccatatttc
     8701 cgtgacgcct ggcggaccat gccgagatat ttgtgcgcac gtttatcaag gtggttcggt
     8761 accatgttaa tggtgacgct gtagatgata cgcagcccgt tattgcggct ttgcagacgg
     8821 tttttcagca gataatccgc gaagttgctt gcgcgacctt cccagcgaat cggctgcccg
     8881 gtgttgtcca ccacctgacc gtaaaggaaa ggttctttcg aaatcgtaat atcatcaacg
     8941 tcggaataga gatcgaccga actcggatcg tcacccatca gcaacgtttt gataattgcc
     9001 cggcgcaaat gcgcggcagg ttctgtcccg gcgatagttt cgatagtaat cgtaccgtca
     9061 tcgaagttga tgtggctgcg ggtctgatat tgatcggtgt atttcacgta gtccttagga
     9121 ccggcgatca ccacctcttt gaatccccag atgttctcaa tattgtgggc aaattgcccc
     9181 atcagaatat caaaaccgtt ggtatctttg acccaggctt cgttataggt atcgcctttt
     9241 ttggtcgtcg aacaggagat gagcaacggc gcaatcaaag ccagcgcgag atattttttc
     9301 atcattccgg gtgcgtgttg tgtttgtttt tatgcaagtt gccggaggcg tgctccggca
     9361 ctgtttttat tttttatctt ccggcgtata gccctcgata tgcacctctt taccctcgaa
     9421 caggaagttg accatctcct gctcaagcag cttgcggtgc tcggcattca tcatgttgag
     9481 tttcttttca ttaatcagca tggtttgctt gtgctgccac tgcgcccagg cttctttgga
     9541 gatctcgtta tagatgcgtt ttcccagctc gccggggtac agctgaaaat cctgaccttc
     9601 tgcttcacgt tgcaggaaag tacaaaaaat cgttctgctc ataaatcatc ctctttatcg
     9661 actcacgcgc taaaccggcg cgccagtgcg taactgctgt aacaaacgct ccacgggagc
     9721 cgccaggcca actgacggcg gttgcgctaa gttataccag agcgcattgc cttcatccat
     9781 gcagccggtg aatgacgaca cgggaagcca cataggcaca atatctaagt ggaaatggct
     9841 gaaggtatgg cgaaacgcgg tcagctgcgt caggttatcg gcagcaatct gccgttgcgc
     9901 cagccaatgc cgcaaacttt cttcgtcggc aaactgcggg aaacagtata aaccgcccca
     9961 caatccgctc ggcggacgct gcgccagcaa tacttcatct tcgtgctgta atagcaaaaa
    10021 gtagccggtg cgctccggca gcgtctgttt cggttttttg cccggataaa gcgaccagct
    10081 attgttggcg gcggcaatac atccgttttg tagcggacag agcgaacatt tcggtttcga
    10141 gcgcgtacaa atcatcgcgc ccaaatccat catcgcctga ttaaaccgtt ccacgccgac
    10201 cgcgggcgtc acctgctcgc ttaaactcca taatttattc tcgacctctt ttttcccagg
    10261 ccagccgctt acagcatagc agcgcgccag cacgcgtttg acgttaccgt cgagaatcgg
    10321 aaagtgctta cccagagaaa gcgagagaat cgcgcctgcg gtggaacgcc cgacgcccgg
    10381 cagtgctgca acttcctcaa aggtttccgg gaatttaccg ccgtgtaagg tcgccacttg
    10441 ttgtgccgct ttatgcagat tgcgcgcgcg ggcgtaatag ccaagcccgg tccacaagtg
    10501 gagaacttcg tcgagcggcg cattggcgag atcggtcacc gtcgggaagc gcgccataaa
    10561 gcgttcaaaa taggggataa cggtcgcaac ctgagtttgt tgcaacatca cttctgagag
    10621 ccatactttg tagggcgtct tgtcaatttg ccagggcaga gtttttcgcc cgtatttatc
    10681 gtaccagtcc agaacctggg ctgaaaattg cgacgcttgc atggtcaccg aattcactgt
    10741 tgttgggggc aagattgcag cacagcgacg gcagggtgta aaccggaact ttccgcagcc
    10801 acggccttat catgcttgca tccggcaact aactttggat aatgcccgtt ttcagaacac
    10861 tttcacaagc gactaaatct ttatgaaaaa cgacgtcatt tcaccggaat ttgatgaaaa
    10921 cggccgccca ctgcgccgta tccgtagttt tgtgcgccgc caggggcgac tgaccaaagg
    10981 ccaggaacat gcgctggaaa actactggcc ggtgatgggc gttgagttca gcgaagatat
    11041 gctggatttc cccgcgcttt ttggccgtga agcgccggtg acgcttgaga ttggttttgg
    11101 catgggggcg tcgctggtgg caatggctaa agatcgcccc gagcaggact tcctcggcat
    11161 tgaagtgcat tcaccgggcg ttggtgcgtg cctggcttct gcgcatgaag agggcttaag
    11221 caacctgcgc gtgatgtgtc acgatgcggt tgaagtgctg cataaaatga ttcctgacaa
    11281 ttcattgcgc atggtgcagc tctttttccc tgacccgtgg cacaaagcgc gccataataa
    11341 acgccgtatc gttcaggtgc cgtttgccga actggtaaaa agcaaactgc agctgggggg
    11401 cgtattccat atggcgaccg actgggaacc ttatgcggaa catatgcttg aagtgatgtc
    11461 ttctattgac ggttataaaa acctgtcaga gagcaatgat tacgtaccgc gtccggcatc
    11521 acgtccagtg acgaaatttg aacaacgtgg tcatcgtctt ggtcacggag tatgggactt
    11581 aatgttcgag agggtgaaat aatggcaaag aaccgtagcc gtcgtctgcg taaaaaaatg
    11641 cacatcgacg aattccagga attaggattt tcggtggcat ggcgattccc ggaaggtaca
    11701 tcggaagaac agattgataa aaccgttgat gattttatta acgaggttat cgaaccgaac
    11761 aaactggcct ttgacggcag cggttatctg gcctgggaag gtctgatctg catgcaggaa
    11821 atcggcaaat gcaccgaaga acatcaggca attgtgcgta agtggctgga agagcgcaaa
    11881 ctggatgagg tacgcaccag cgaacttttc gacgtttggt gggactaaga aagcatacgg
    11941 gcgatgacaa atgcaaaact gcctgatgcg ctacgcttat catgcctgga aagatgcacg
    12001 atcgagtagg ccggataagg cgtttacgcc gcatccggca tggaaaacgc gtactttgtt
    12061 atcaatctgg ggccagcaaa tgctggcctg atttgttctt gagggaagac tatgatgcgc
    12121 aaaatgctgc tggcggcagc actttcagtg acggcaatga ccgctcacgc cgactaccag
    12181 tgcagcgtca cgccgcgtga cgatgtgatt gtcagcccgc aaaccgtgca ggtgaagggc
    12241 gaaaacggca atctggtgat cacgccagac ggcaacgtga tgtataacgg taagcaatat
    12301 tccctgaatg ccgcccagcg cgagcaggcg aaggattatc aggctgaact acgtagcacg
    12361 ctgccgtgga ttgatgaagg cgcgaaaagc cgcgtcgaga aagcccgtat tgctctggat
    12421 aaaattatcg ttcaggagat gggcgaaagc agcaaaatgc gcagccgtct gaccaaactt
    12481 gatgcgcagc tgaaagagca gatgaaccgc attatcgaaa cgcgcagcga tggcctgacg
    12541 tttcactata aagccattga tcaggttcgc gccgaaggcc agcaattagt gaatcaggca
    12601 atgggcggaa ttttacagga cagcattaat gaaatgggcg cgaaagcggt gctgaaaagc
    12661 ggcggtaacc cattacagaa tgtgctggga agcctggggg ggctgcaatc ctcaatccaa
    12721 accgagtgga aaaagcagga aaaagatttc cagcagtttg gcaaagatgt ttgtagccgt
    12781 gttgtgactc tggaagatag ccgcaaagcc ctggtcggga atttaaaata attctctatt
    12841 ttaagacggc ataatacttt tttatgccgt ttaattcttc gttttgttac ctgcctctaa
    12901 ctttgtagat ctccaaaata tattcacgtt gtaaattgtt taacgtcaaa tttcccatac
    12961 aaagctaagg gataatgcgt agcgttcacg taactggagg aatgaaatgg agtttttcaa
    13021 aaagacggca cttgccgcac tggttatggg ttttagtggt gcagcattgg cattacccaa
    13081 tatcaccatt ttagcaaccg gcgggaccat tgccggtggt ggtgactccg caaccaaatc
    13141 taactacaca gcgggtaaag ttggcgtaga aaatctggtt aatgcggtgc cgcaactgaa
    13201 ggacattgcg aacgttaaag gcgagcaggt agtgaatatc ggctcccagg acatgaacga
    13261 taatgtctgg ctgacactgg cgaaaaaaat taacaccgac tgcgataaga ccgacggctt
    13321 cgtcattacc cacggtaccg acacgatgga agaaactgct tacttcctcg acctgacggt
    13381 gaaatgcgac aaaccggtgg tgatggtcgg cgcaatgcgt ccgtccacgt ctatgagcgc
    13441 agacggtcca ttcaacctgt ataacgcggt agtgaccgca gctgataaag cctccgccaa
    13501 ccgtggcgtg ctggtagtga tgaatgacac cgtgcttgat ggccgtgacg tcaccaaaac
    13561 caacaccacc gacgtagcga ccttcaagtc tgttaactac ggtcctctgg gttacattca
    13621 caacggtaag attgactacc agcgtacccc ggcacgtaag cataccagcg acacgccatt
    13681 cgatgtctct aagctgaatg aactgccgaa agtcggcatt gtttataact acgctaacgc
    13741 atccgatctt ccggctaaag cactggtaga tgcgggctat gatggcatcg ttagcgctgg
    13801 tgtgggtaac ggcaacctgt ataaatctgt gttcgacacg ctggcgaccg ccgcgaaaaa
    13861 cggtactgca gtcgtgcgtt cttcccgcgt accgacgggc gctaccactc aggatgccga
    13921 agtggatgat gcgaaatacg gcttcgtcgc ctctggcacg ctgaacccgc aaaaagcgcg
    13981 cgttctgctg caactggctc tgacgcaaac caaagatccg cagcagatcc agcagatctt
    14041 caatcagtac taatcacctc gccccggtat cgtgccgggg ctttttcact tcagacttac
    14101 gtccattgcc aattttcatt accctaatga taatcaccgg aataaattat tccgcgtgag
    14161 gtttttcggg tgaaaaagca atggattgtt ggcacggcac tgcttatgtt gatgactggt
    14221 aatgcctggg cagatggcga accgccaact gaaaatatct taaaagatca attcaaaaag
    14281 cagtatcacg gcattctcaa gcttgatgct atcaccttaa aaaatcttga tgctaagggt
    14341 aatcaggcca cctggtcagc ggaaggcgat gtctcttcca gtgacgatct ctatacctgg
    14401 gtcggtcagt tggcagatta cgaactgctc gaacagacct ggacgaaaga taaaccggta
    14461 aaattctcgg cgatgttaac cagtaaagga acgcccgcgt ctggctggtc ggtgaacttt
    14521 tactcttttc aggcggcagc cagcgatcgt gggcgggtgg tcgacgatat caaaacgaat
    14581 aataaatatc tgatcgtgaa tagcgaagat ttcaattatc gctttagtca gcttgaatct
    14641 gcgttgaata cccagaagaa ctcgattcct gcgctggaaa aggaagtgaa agcgcttgat
    14701 aagcaaatgg tggcggcgca gaaagcagcg gatgcatatt ggggaaaaga tgcaaacggt
    14761 aagcaaatga cccgcgaaga agcctttaaa aaaattcacc aacagcgtga tgagtttaat
    14821 aaacagaacg atagcgaggc gttcgcggtt aaatatgaca aagaggttta tcaaccggcg
    14881 attgcggcat gtcataaaca gagtgaagag tgttatgaag tgccgattca gcagaaacga
    14941 gatttcgata tcaacgaaca acggcgacag acttttctgc aatcacaaaa actcagccgt
    15001 aaattgcagg atgactgggt aacgctcgaa aaagggcaat atccgttaac gatgaaagtt
    15061 tcagaaataa acagtaaaaa agtcgcgatt ttgatgaaaa ttgacgatat caaccaagct
    15121 aatgaacgct ggaaaaaaga taccgaacaa ctgcggcgca atggggtgat taagtaagtt
    15181 tgatatagtg cacgatttgt agcaggatgc ggcgttgacc ccgcatccgg caatcaacgt
    15241 ctgatgcgac gcttgcgcgt cttatcagac ctacaaccgt tgccacatgt aggctggata
    15301 aggcgttcac gccgcatccg gcaatacaag tttactcagc cagaaaaagc tccagcagcg
    15361 aatttaaaaa cagcttccca tgttccgtta tctgccagta atccgcacat tctgtgagat
    15421 aaccctgggc aatcgcctcg tctaactgtg ggcgaatcac atcttcgcaa agcccggtat
    15481 acgcaataaa ctccacgcgc ggcgcggcct ccagcagacg gaagcgattc ataaagaact
    15541 caaacggctt atctgcggct tcgacatcac gctggctttc cagatacctt ccttgcataa
    15601 aaccacgcgg atgacgcgtt ttggtggtac gcagaatgcg cccatccggg aaggtcactt
    15661 tcccgtgcgc gccgcagcca ataccgatgt agtcgccaaa gcgccagtag ttgagattgt
    15721 gctggcactg ataaccgggt ttggcgtaag cggaagtttc atattgctga taacccgctg
    15781 cggttaataa ctgatgcccc tgttcgaaaa tatcccacaa cgcatcgtcg tccggcagca
    15841 ccggtggtcg cgaaccaaac agcgtattgg gttcgatggt cagttgatac caggaaagat
    15901 gcggcggatt cagttcaatg gcctggcgta gatcgccaag cgcctcttcc agtgattgat
    15961 ccggtagtcc atgcatcaaa tcaaggttaa agctacgtaa ccctaaaccg ctcgccagct
    16021 ttgccgctcg tttcgcttct tgcgggccat gaatacgccc aagtcgtttc agcttttctt
    16081 cgctaaaact ctgcacacca atagagatgc ggttcacacc agcacgctga taatcgacaa
    16141 agcgatccgc ttctaccgtg cctgggttcg cttccatagt aatttctgca tccgctgcca
    16201 gcggcaaacg cgcacgcacg ccgtccagca gcgtttgcat cgccgggccg gaaagcaggc
    16261 tcggcgtacc accgccaata aagattgtct ttacttcacg gccctgagcg taagccacat
    16321 cgttgtccag gtcgttaagc agatgctgaa cataatcgtc gtgcggcact tctcctttca
    16381 acgcgtgaga gttgaaatcg cagtacgggc atttctgcac gcaccacggg atgtgaatgt
    16441 agagacttag cggcggtaat ttaaccatta cgtaaagcgt ccagcagcag tttcaatgcc
    16501 tgaccacggt gggaaatggc gctcttttct tcgcgggtca gttcggcagc ggttttccct
    16561 tcggaaggta cgaagaagat tggatcataa ccaaagccgc cagtacccgc tggttcacga
    16621 gtaatcatgc ccggccagct gccgtggcac accagcggag tgggatcttc cgcgtgacgc
    16681 agatatacca gtacgcagtg gaaacgcgcc tgacgttggt tgtccggtac gtcttttagt
    16741 gtttccagca gtttttgcag attcttttga tcggtcgcgt cttcaccgga ataacgcgcg
    16801 gagtaaatgc ccggcgcgcc gccaagtaca tctaccgcca gaccagagtc gtcggcaatt
    16861 gccggtaaac cggtcacttt tgccgcatgg cgcgctttca gaatcgcgtt ttcgataaag
    16921 gtcaggccgg tttcttcagc ggaatctacg ccgaggtctg tttgggccac gatatcaaga
    16981 ccgaagtcgc taagcagcga cgccagctca cgcactttac cggcattgcc ggttgcgagg
    17041 acaacttttt gcataggata cctaattaat taacgccgcg atttctggcg ggatttgttg
    17101 cggattaatg attttaattt gtttgtggcg gccaagttcg cctttttcaa tcaccacctg
    17161 gcttttggca acccggaatt gcttaccgag aaacttcacc agatggctgt tggcctgacc
    17221 gtcaaccggc ggcgcggtaa tggcgacttt aacttcgtcg ccatgtaaac cgacaataga
    17281 atcacggctg gctttcggct gaatataaag ccgtaaaacc agaccgtcat cattaactgt
    17341 tacggcactc ataacgccat ccacagcccc ggcagcagca tgtttccggt tgcctgtaat
    17401 acttctgcga cacccatatt gatgacatac agcagcagaa cgaggatcat cggcgagaaa
    17461 tcaatcccac ccattgccgg tagcaggcgg cgaatcgggc gcagcagcgg atcggccagc
    17521 tgaatcagca cgtattcaat cggactacga ccctggctta cccagctcat aatcgccatc
    17581 accagcagga cccagaaaat cagcaggccg atggttttca gcagaatcag taaaccggca
    17641 atccagatga ttggcaggaa ggtcaccact ttaaacagca cgatggcttt gataaaactg
    17701 agaatgtacg caaccagcag cgaggcgcta tcaatgggac ccattgccgg aataacgcgg
    17761 cgcagtggcc cgataattgg ctgcgttacc ttcactacaa actgtgagaa ggggttgtaa
    17821 aaatcacaat gagcccactg catccagatg cgtaataaca gcaccatggt atacagctca
    17881 atgaccgttg aaagcaggaa agtcaacgta ttcatggcgt tcctttaatt ccttattttt
    17941 tagagtaatc acgcgcacca aaaattgcag tgccgatacg aaccatcgtg ctacctgccg
    18001 caatggcggc ttccatatcg tccgacattc ccagagagag cgtgtcgata tgcgggtagc
    18061 gcgttttcag tccggcaaat gctacagcca tttggcgtgc aacttcaaac tgccttacat
    18121 attctgactc aggcgcaggg attgccatca gcccgcgcag acgtaaacgc ggtagttcag
    18181 cgaccgccgc tgccagctcg tccagttctg ccagttgaat cccggactta ctgttttcat
    18241 cactaatatt aatttgaatc agtacgttaa gagggggaag ttctgccggg cgctgatcgt
    18301 tgaggcgggt agcaatgcgc aaacggtcga tggtatgaca ccagtcgaaa tgctctgcca
    18361 ccaggcggct tttattagac tgcaacgggc caataaaatg ccattctaat cctgttacgc
    18421 ccagttcctg aaagtggcga attttatcta ccccttcctg aacgtagttt tcaccaaatt
    18481 gacgctgccc ggcatcaatg gcttctgcga tggcgctcgc aggttttgtt ttactgactg
    18541 caagcagcgt aatttcttct ggagaacggc cgcaacgcgt tgcagccgct gagattttgt
    18601 cccggacctg tgccaggtta tgcgcaatat cgttcatttt ccgaggatct tagtatgaat
    18661 atggaagaaa ttgtggccct tagtgtaaag cataacgtct cggatctaca cctgtgcagc
    18721 gcctggcccg cacgatggcg cattcgcggg ctaatggaag ctgcgccgtt tgatgcgccg
    18781 gacgtcgaag agctactgcg ggagtggctg gatgacgatc agcgggcaat attgctggaa
    18841 aatggccagc tggattttgc cgtgtcgctg gcggaaaacc agcggttgcg tggcagtgcg
    18901 ttcgcgcaac ggcaaggtat ttctctggca ttacggttgt taccttcgca ctgtccacag
    18961 ctcgaacagc ttggcgcacc aacggtattg ccggaattac tcaagagcga gaatggcctg
    19021 attctggtga cgggggcgac ggggagcggc aaatctacca cgctggcggc gatggttggc
    19081 tatctcaatc aacatgccga tgcgcatatt ctgacgctgg aagatcctgt tgaatatatc
    19141 tatgccagcc agcgatgttt gatccagcag cgggaaattg gtttgcactg tatgacgttc
    19201 gcatcgggat tgcgggccgc attgcgggaa gatcctgatg tgattttgct cggagagctg
    19261 cgtgacagcg agacaatccg tctggcactg acggcggcag aaaccgggca tttggtgctg
    19321 gcaacattac atacgcgtgg tgccgcgcag gcagttgagc gactggtgga ttcatttccg
    19381 gcgcaggaaa aagatcccgt acgtaatcaa ctggcaggta gtttacgggc cgtgttgtca
    19441 caaaagctgg aagtggataa acaggaagga cgcgtggcgc tgtttgaatt attgattaac
    19501 actcccgcgg tggggaattt gattcgcgaa gggaaaaccc accagttacc gcatgttatt
    19561 caaaccgggc agcaggtggg gatgttaacg tttcagcaga gttatcagca gcgggtgggg
    19621 gaagggcgtt tgtgacgttg ccatgcccga tgcgcaagtt tat
//
LOCUS       DAZGAN020000087        19516 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGAN020000087 DAZGAN020000000
VERSION     DAZGAN020000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.087"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            40..576
                     /gene="yfdR"
                     /locus_tag="ACHKYO_RS17330"
                     /old_locus_tag="pgaptmp_003466"
     CDS             40..576
                     /gene="yfdR"
                     /locus_tag="ACHKYO_RS17330"
                     /old_locus_tag="pgaptmp_003466"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4430248.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSELRKCA"
     gene            567..929
                     /locus_tag="ACHKYO_RS17335"
                     /old_locus_tag="pgaptmp_003467"
     CDS             567..929
                     /locus_tag="ACHKYO_RS17335"
                     /old_locus_tag="pgaptmp_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430249.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWLWTFQVGDLE
                     NVNITHWAVMPKAPKNKK"
     gene            926..1129
                     /locus_tag="ACHKYO_RS17340"
                     /old_locus_tag="pgaptmp_003468"
     CDS             926..1129
                     /locus_tag="ACHKYO_RS17340"
                     /old_locus_tag="pgaptmp_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430250.1"
                     /translation="MSVIKTHTGIVITRDGPQVKKLHQTKRMWVVGKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPIEVKHV"
     gene            1122..1361
                     /locus_tag="ACHKYO_RS17345"
                     /old_locus_tag="pgaptmp_003469"
     CDS             1122..1361
                     /locus_tag="ACHKYO_RS17345"
                     /old_locus_tag="pgaptmp_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430251.1"
                     /translation="MFKQNEKSIAQIAEYIPRACRDMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNSHTRKSFFSCITAQIRKGGNQ"
     gene            1358..1810
                     /locus_tag="ACHKYO_RS17350"
                     /old_locus_tag="pgaptmp_003470"
                     /pseudo
     CDS             1358..1810
                     /locus_tag="ACHKYO_RS17350"
                     /old_locus_tag="pgaptmp_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            1908..2086
                     /locus_tag="ACHKYO_RS17355"
                     /old_locus_tag="pgaptmp_003471"
                     /pseudo
     CDS             1908..2086
                     /locus_tag="ACHKYO_RS17355"
                     /old_locus_tag="pgaptmp_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3098..3169
                     /locus_tag="ACHKYO_RS17360"
                     /old_locus_tag="pgaptmp_003472"
                     /pseudo
     CDS             3098..3169
                     /locus_tag="ACHKYO_RS17360"
                     /old_locus_tag="pgaptmp_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            3263..3505
                     /locus_tag="ACHKYO_RS17365"
                     /old_locus_tag="pgaptmp_003473"
     CDS             3263..3505
                     /locus_tag="ACHKYO_RS17365"
                     /old_locus_tag="pgaptmp_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4430252.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRITEHSVFFRRMDGRSGRVRIG
                     ELNCLFEHIDHQEYRKILADTEQEKHLKKLRAIKRK"
     gene            3509..3643
                     /locus_tag="ACHKYO_RS17370"
                     /old_locus_tag="pgaptmp_003474"
     CDS             3509..3643
                     /locus_tag="ACHKYO_RS17370"
                     /old_locus_tag="pgaptmp_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430253.1"
                     /translation="MNKAFERWVHQRYGNRYDLTRDVDGFYCREVVKRMVEVWCHCRG
                     "
     gene            3662..3916
                     /gene="xisR"
                     /locus_tag="ACHKYO_RS17375"
                     /old_locus_tag="pgaptmp_003475"
     CDS             3662..3916
                     /gene="xisR"
                     /locus_tag="ACHKYO_RS17375"
                     /old_locus_tag="pgaptmp_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4430254.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            3950..5236
                     /locus_tag="ACHKYO_RS17380"
                     /old_locus_tag="pgaptmp_003476"
     CDS             3950..5236
                     /locus_tag="ACHKYO_RS17380"
                     /old_locus_tag="pgaptmp_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4430255.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHINAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKVG"
     gene            5272..5958
                     /gene="mlrA"
                     /locus_tag="ACHKYO_RS17385"
                     /old_locus_tag="pgaptmp_003477"
     CDS             5272..5958
                     /gene="mlrA"
                     /locus_tag="ACHKYO_RS17385"
                     /old_locus_tag="pgaptmp_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HGU4430256.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            6018..6125
                     /gene="yohO"
                     /locus_tag="ACHKYO_RS17390"
                     /old_locus_tag="pgaptmp_003478"
     CDS             6018..6125
                     /gene="yohO"
                     /locus_tag="ACHKYO_RS17390"
                     /old_locus_tag="pgaptmp_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HGU4430257.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(6106..6837)
                     /gene="yehW"
                     /locus_tag="ACHKYO_RS17395"
                     /old_locus_tag="pgaptmp_003479"
     CDS             complement(6106..6837)
                     /gene="yehW"
                     /locus_tag="ACHKYO_RS17395"
                     /old_locus_tag="pgaptmp_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HGU4430258.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(6842..7768)
                     /gene="yehX"
                     /locus_tag="ACHKYO_RS17400"
                     /old_locus_tag="pgaptmp_003480"
     CDS             complement(6842..7768)
                     /gene="yehX"
                     /locus_tag="ACHKYO_RS17400"
                     /old_locus_tag="pgaptmp_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HGU4430259.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALVEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(7761..8918)
                     /gene="yehY"
                     /locus_tag="ACHKYO_RS17405"
                     /old_locus_tag="pgaptmp_003481"
     CDS             complement(7761..8918)
                     /gene="yehY"
                     /locus_tag="ACHKYO_RS17405"
                     /old_locus_tag="pgaptmp_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HGU4430260.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(8925..9842)
                     /gene="osmF"
                     /locus_tag="ACHKYO_RS17410"
                     /old_locus_tag="pgaptmp_003482"
     CDS             complement(8925..9842)
                     /gene="osmF"
                     /locus_tag="ACHKYO_RS17410"
                     /old_locus_tag="pgaptmp_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HGU4430261.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(10053..12350)
                     /gene="bglX"
                     /locus_tag="ACHKYO_RS17415"
                     /old_locus_tag="pgaptmp_003483"
     CDS             complement(10053..12350)
                     /gene="bglX"
                     /locus_tag="ACHKYO_RS17415"
                     /old_locus_tag="pgaptmp_003483"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HGU4430262.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            12546..14261
                     /gene="dld"
                     /locus_tag="ACHKYO_RS17420"
                     /old_locus_tag="pgaptmp_003484"
     CDS             12546..14261
                     /gene="dld"
                     /locus_tag="ACHKYO_RS17420"
                     /old_locus_tag="pgaptmp_003484"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU4430263.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(14299..15231)
                     /gene="pbpG"
                     /locus_tag="ACHKYO_RS17425"
                     /old_locus_tag="pgaptmp_003485"
     CDS             complement(14299..15231)
                     /gene="pbpG"
                     /locus_tag="ACHKYO_RS17425"
                     /old_locus_tag="pgaptmp_003485"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HGU4430264.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(15405..15992)
                     /gene="yohC"
                     /locus_tag="ACHKYO_RS17430"
                     /old_locus_tag="pgaptmp_003486"
     CDS             complement(15405..15992)
                     /gene="yohC"
                     /locus_tag="ACHKYO_RS17430"
                     /old_locus_tag="pgaptmp_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HGU4430265.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLDIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            16162..16740
                     /gene="yohD"
                     /locus_tag="ACHKYO_RS17435"
                     /old_locus_tag="pgaptmp_003487"
     CDS             16162..16740
                     /gene="yohD"
                     /locus_tag="ACHKYO_RS17435"
                     /old_locus_tag="pgaptmp_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4430266.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHTLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(16870..17631)
                     /gene="yohF"
                     /locus_tag="ACHKYO_RS17440"
                     /old_locus_tag="pgaptmp_003488"
     CDS             complement(16870..17631)
                     /gene="yohF"
                     /locus_tag="ACHKYO_RS17440"
                     /old_locus_tag="pgaptmp_003488"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4430267.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSYVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(17684..19120)
                     /gene="mdtQ"
                     /locus_tag="ACHKYO_RS17445"
                     /old_locus_tag="pgaptmp_003489"
     CDS             complement(17684..19120)
                     /gene="mdtQ"
                     /locus_tag="ACHKYO_RS17445"
                     /old_locus_tag="pgaptmp_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HGU4430268.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGCSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            19344..19427
                     /gene="yohP"
                     /locus_tag="ACHKYO_RS17450"
                     /old_locus_tag="pgaptmp_003490"
     CDS             19344..19427
                     /gene="yohP"
                     /locus_tag="ACHKYO_RS17450"
                     /old_locus_tag="pgaptmp_003490"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HGU4430269.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         4964 a         5094 c         4922 g         4536 t
ORIGIN      
        1 aaattcagcg cggtgcagcg catatacgtg gagaacaaaa tgtcatttat taaaactttt
       61 tccgggaagc atttttatta tgacaagata aataaagatg acatcgttat taacgatatc
      121 gcggtttccc tttcaaatat ctgtcgcttt gccggtcatc tttctcactt ctacagcgtc
      181 gcccaacatg cggtgctttg cagccagctg gtgccgcagg aatttgcttt tgaagcgtta
      241 atgcatgatg caacagaagc gtattgccag gacatcccag ctccactgaa acgccttctt
      301 cctgactata aacggatgga agaaaaaata gacgccgtaa tccgtgagaa atacgggtta
      361 cctcctgtta tgagcacgcc agtgaaatat gccgatctca ttatgctggc aaccgaacgt
      421 cgcgatctcg ggcttgatga tggctctttc tggcctgtac tggaaggtat cccggcgaca
      481 gagatgttca aagttattcc actgtcacca ggccatgcct acgggatgtt tatggaacgt
      541 tttaacgagt tatcggagtt acgcaaatgc gcatgaatgt tttcgaaatg gaagggtttc
      601 ttcgcgggaa atgtgtacca cgagatctga aagtgaatga aacaaatgct gagtacctgg
      661 tacgtaaatt cgatgcgctt gaagctaaat gtgcggcact ggaaaacaaa ataataccag
      721 tgtcagctga actgccgcca gcaaatgaaa gtgttctgtt atttgatgct aacggagaag
      781 gctggctaat tggctggcgt tctctctggt acacctgggg acaaaaagaa accggagaat
      841 ggctgtggac atttcaggtc ggggaccttg aaaacgtcaa tatcactcac tgggcagtaa
      901 tgccgaaagc accgaagaat aaaaaatgag cgtgataaaa actcatacag gaattgttat
      961 cacccgagac ggtccgcagg taaaaaaact gcaccagaca aagcggatgt gggtcgtcgg
     1021 aaaaaacgag ttttaccaca aagaaaccgg acgccgccac tttgcagaaa atactcgccg
     1081 ccgactgctg atcgatacca tcaagcctat cgaggtgaag catgtttaaa cagaacgaaa
     1141 aatctatcgc tcaaattgct gagtatatcc cgcgtgcgtg ccgggatatg cagttgcagg
     1201 aagccaaagc acgcctggag aaaaaaattg cgctctatat cgatgacggc tgtgatgccg
     1261 ccgttcttaa cgcggcgttc gcgccagctc ttaacagtca tacgcgaaag tctttttttt
     1321 cgtgcatcac agcgcagatc cgtaaaggag gcaaccagtg agcaacatta actatcaggt
     1381 actgcgtgaa aaggcagaga aagcaactaa aggaagctac atcgtagggc atacatctgt
     1441 taaccaacac ggcaatttaa caggagtttt tgtttgtcaa aaatggaaag gagaacccgg
     1501 tggcgtaatt gcagaatgtc acgttaactg cctggttgaa acagatgctc aggcttatgc
     1561 aaacgctgaa ttcatagcag aggctaaccc ggctaccgtg ctggcactgc tggatgaacg
     1621 ggaaagaaac ctgcaataca tcaaaagccg cgatcaggag aacgaggata ttgcgctaac
     1681 ggtagggaag ctgcgcgttg agcttgaagc agaaaaacag cgggcaaaag ttctatttat
     1741 ggaaaatgct cggcttaagt caggcatagc cggtctgata cacctcggta ttcgatatgc
     1801 agatgttgag gtcatgaaaa ttgctggaga tgcccagctt tctaccccat gcactgacag
     1861 catcataaac agcattgcaa caggcattcg catcaaagga gagtgatatg gcgttaacac
     1921 accacgaact ctgtcagatt gcgtacaagt tccttaagcg caacgggttc aaggtttgct
     1981 ttcatgaccg ctttgttgct gtaaccagta ccggagaaca gccagatgct atgggattca
     2041 gaaattcagc atcatgcctg atagaggcga agtgttctcg tgctgacttg ttggcagata
     2101 gaaaaaagcg tttccgtaaa aatccctcac ttggcatggg cgactggcga ttctttatta
     2161 gtgagccgga aattatttca gttgaggatt tacctcccgg ctggggatta cttcacgttg
     2221 ttaacggaag agtacggaaa gtacatggat ggcccagggg taattgctgt tggggtaatc
     2281 ctgacgataa gccatttact gggaataagc aggttgaatg cgattacatg ttatctgcat
     2341 taaggcgcat ggagttgaga gggcacctta atgaaatata tgacggtgtg attgttaata
     2401 agaaagaagg aaacgcggca tgatcactat taccaaaggg cgactgctga caatcaagca
     2461 gtggcgcgaa acatacggac cgggtagcaa cgttgtactg ccagcagaag aagcggaaga
     2521 actggcacga attgcactgg tatcgctgga agcagagccg gtggcaaaga ttatagctca
     2581 ttacccatta ggagttgacg taggcaaaca aaagttcgta caggccattg gagagcttcc
     2641 tgactttggc ggatatctat ttgccgcccc gccagcgccg gtagtgccgg aagaagcaac
     2701 tccggaaaac gtagaaatgc tctctggcta tgtttccacg tacaaattaa ccgatagcga
     2761 gcgcgatatt gctgccgaaa tatggaacgc ctgccgcacc gccatgcttc agtccggaaa
     2821 ctttcgggaa atcaagaatt cgtcaaccaa taattttcgg gaaatcccgg aagcgtcaac
     2881 cagctctccg gtaactccgg ctcttctgcc tggtggtttc accattgagg aggcgaagga
     2941 attacatgaa gacctggtac gcagccacat aagcaaggcc ttaagtggcg aaaagatgaa
     3001 aaagaaagat cgcgatgctg atttgcgctg gattcatggc gttatagttc aggcagcgtg
     3061 gtttgtaaaa gcatcactgg agcagaatgc actatcgggc aactctccgg taactccgga
     3121 tggttggata agctgtagtg agcgaatgcc ggacgacagg caggaggtga atcaatgagc
     3181 tggcctgatg caatcgtaac tctgggggtg gtattcgcag cagcgtttgt tgtgttctcg
     3241 atttgtcgat ggggataacc acatgttcgc tttgattcaa cgcggtcaga tatacacgga
     3301 cagagctgga taccccgtgg tgattactcg catcactgag cactcagtgt tctttcgacg
     3361 gatggacgga cgatccgggc gggtacgcat tggtgagtta aactgcctgt tcgaacatat
     3421 tgaccaccag gagtaccgca aaattctcgc ggacactgag caggaaaagc acctgaaaaa
     3481 attacgagcc ataaaaagga agtaaagaat gaataaagca tttgaacgat gggtccacca
     3541 gcgttacggc aatcgctatg acctgacgcg agatgttgac ggcttctact gtcgtgaagt
     3601 tgtgaagcga atggttgaag tgtggtgcca ctgccgtgga tgaaaatttt atgaggttgg
     3661 catgcagaca atcatctatc agataacccc cagcaaatgg tgtacggaga gagtcctcat
     3721 tgcatcaaca gggctaaagc ctggcaccat tgagcgggca agaagaaagt catggatgca
     3781 gggaaaagaa taccgccatt acgctgtaga aggtgatccg gggcactaca gtgaatgcct
     3841 gtacaacatc gaagaaatta tgcgatggat cgaaaaccag aaacaaccag gtgccaaaaa
     3901 tgcaagttcc ggttaacctg ttaatgctcc tggacgtctg ggaggtttaa tgagtaacgc
     3961 atcatacccg acaggcgttg aaaaccatgg aggatcactc cgtatatggt ttcactataa
     4021 tggcaaacgt gtcagagaaa acctcggtgt tcctgacaca gccaaaaacc ggaagatcgc
     4081 tggtgaactt cgcacttccg tttgttttgc aatcagaatg gggagtttcg actacgccgc
     4141 gcagttccct aattccccta acctgaaaca ctttggtctg ggaaaaagag agataaccgt
     4201 taaggcactt tcggaaaaat ggttggacct taagaaaatt gagatttgtg cgaatgcact
     4261 taaccgttac cagtcagtaa ttaaaaacat gttaccaatg ttaggtgaaa aaaaactggt
     4321 ttcatccata acaaaagagg atttactttt cgtaaggaga gatttgttga ccggttacca
     4381 aaagctttct aatggaaaga cttcttccat aaaagggcgc tcagtggtca cggtaaacta
     4441 ctatatgaca accatagctg gaatgtttca atttgcaaca gataatggtt atacctcagg
     4501 aaacccattt aacggtctgg ctcccttaaa aaagtccaag gtaaaaccag atcctctcac
     4561 ccgtgacgaa tttattcgtt ttattgaggc ttgccgtcat caacaaacaa aaaacctgtg
     4621 gattctcgct gtatacacgg gtattcgtca cggggagttg gtatcgctgg catgggaaga
     4681 tatagacctt aaagcaagga ctataaccat ccgtagaaat tatacaaaac ttggcgaatt
     4741 cactccacca aaaaccgatg caggcaccgg aaggacaatt catctggttc aaccagctat
     4801 tgatgctctt aaaagccagg cggaaatgac catgcttgga aagcaacatt ctgtagaggt
     4861 gaagcagagg gaatatggga gaactgctgt gcataaatgc acttttgttt ttagtcctca
     4921 ggtaacaaaa cagcagcagt tgtccggacc tcactacaag gttgactcca tcagggagtc
     4981 atggacaagt atcttaaaac gcgcaggtct gagacacaga aaatcgtacc aatccaggca
     5041 tacttatgca tgctggtcac ttgccgctgg agctaatcct agttttatcg caagccagat
     5101 gggccacata aacgcacaaa tggtattcaa tgtttacgga gcatggatga aagacaacaa
     5161 tcacgaacag atagaactcc ttaacaaaag actatctgaa agtgtcccat gtatgcccca
     5221 taagaaagtg gggtaaaata aaaacttgta aaatcagtta gtttaccctt aatccctgtc
     5281 acgttacgcg cgtggcagag gcgttacggg ttgctgaaac cgcaacggac agacggcggt
     5341 catcgactgt tcaacgatgc cgatattgac cgtatccgcg agatcaaacg ctggatcgac
     5401 aacggcgtgc aggtcagcaa agttaaaatg ctgctcagta atgaaaatgt tgatgtgcag
     5461 aacggctggc gcgatcagca agaaacatta ctgacctacc tgcaaagcgg caatctgcat
     5521 agcctgcgaa cgtggatcaa agagcgcggt caggattacc ccgcccagac actcaccaca
     5581 catctgttta ttcctctgcg ccgacgactt caatgccaac aaccgactct ccaggcgctg
     5641 ctggcgatcc tcgacggcgt actgatcaac tacatcgcca tttgtctggc ttcggcacgt
     5701 aaaaaacagg gtaaagatgc gctggtggtt ggctggaata ttcaggatac cacccgtctg
     5761 tggctggagg gctggattgc cagtcaacaa ggatggcgca ttgatgtcct cgcccactcg
     5821 ctcaatcaac tacgccctga actattcgaa ggccgtacat tgctggtgtg gtgcggtgaa
     5881 aatcgaacct ccgcccaaca gcagcaactc accagttggc aagaacaagg ccatgatatt
     5941 ttcccactcg gcatttaatg attcgttaac aaatgcgctt tactgtacaa tcctttcgtt
     6001 aacataagga gtgcattatg cgcatagcta aaattggggt catcgccctg ttcctgttta
     6061 tggcgttcgg cggaattggt ggcgtcatgc tcgcaggtta tacctttatt ttgcgtgctg
     6121 gctaagcgcc tgcaccagcc tttcaaacag gcggtctgcg atgatcgccg ccagtgccac
     6181 cagtaacgcc ccctggatca catacgcggt attaaatccg ctaagcccga tgatgatggg
     6241 cgtacccagc gtgctggctc ctaccgttga ggcgatcgtc gccgtaccaa tgttgataat
     6301 caccgaagtt cgcacgcccg ccagaatcac cggagccgcc agcggtagct cgaccttacg
     6361 cagtcgctga ccacgactca ttcccatacc tttcgcaact tctgtcacgc tggcatcgat
     6421 cgctcccagc ccggcaagtg tcgcctgcag gacgggcagc acaccgtaaa ggatcaaggc
     6481 gataatcgct ggttgcagac caaagccgat caccggaacg gcaatcgcca gcactgcgac
     6541 aggcggaaaa gtctgtccaa cggcggcaat agtttccacc agtgggcgaa attccgcgcc
     6601 ccacgggcga gtgacagcaa ttccggcacc agtgccaatg atcaccgcaa acaaactcga
     6661 aattcccacc agccagaaat gagccagtgc cagagctgca aaactttctt gctgataaac
     6721 gggtcgtggc agttgtggga acaaggcagc aaacagcggc tggctgtaag gcagccagaa
     6781 aatcagtgcc acaaacaaag caatgagcca gaacagcgga tcgcgcaaca tcttcatacg
     6841 cttacgcctc caccagcaga tcctgaaaat gcagcgtacc gcaaggctgg ccctgcgtgt
     6901 tcaccaccgg cagcacctcg caaccccgcg cgacaaacag ggagagcgca tcgcgtagcg
     6961 tcatctcttc taccagtgcc tcaccttctg cccgttcttc gcgacgcacg taatccgcca
     7021 cactacgtaa cgaaagcaga cgcacaccca gttcgctacg tccaaaaaac tggcggacaa
     7081 aatcattcgc cggacgagtc agcatcgtca gcggattgcc ctgctgcacc acttcaccgt
     7141 gatccatcaa taccagatgt tcagccagcc gtagcgcctc atcaatatca tgagtgacca
     7201 gcacaatagt gcgccccagc aaacggtgaa tgcgcgtcat ctcttgttgc aacgcgccgc
     7261 gcgttaccgg gtccagtgcg ccaaaaggtt catccatcag taagacttgc ggatcggcag
     7321 ccagtgcgcg cgccactccc acacgttgct gctgaccacc ggaaagctga tgcggataac
     7381 gctcacgcaa atttgactcc agccccagta gcgtcattaa ttcgtcgata cgatcgtcaa
     7441 tccgtgcccg cgaccatttt tgtaattgcg gcacggtagc gatgttttgc gccacgctcc
     7501 agtgggggaa caggccaata gattgaatgg catagcccat ccggcggcgc aactccagta
     7561 ctggcagcga gcgaatttct tctccggcaa agcggatctc tccgctgtca tgttccacca
     7621 ggcggttaat cattttcaag gtggtggatt tgccggagcc agatgtgcca atcagcaccg
     7681 aaaaactccc ttcctgaaaa ttgagattaa gatcgttaac ggctttttgt gcgccaaaca
     7741 gtttgctgac atggctaaat tcaatcatta cgtttcacct tcagcagtgc gataagcaaa
     7801 tcgaacaggg catcgatcag caccgccaga acaattaccg ggatcacccc cagcaacact
     7861 aaatcaatgg cgctgcttag cagcccctgg aaaaccagcg caccaaaacc gcctgcgccg
     7921 attaacgccg caatcaccgc catacctaca gtttgcacca tcaccacccg caggctgcgc
     7981 agaaataccg gtaacgccag tggtaactga acatgcagga atcgctgcgc cccgctcatg
     8041 cccatcgctc tggcgctctc cagcacatcg cgcgggatct ggttcaaacc gactaccacg
     8101 ccgcgcacca gcggcagcaa ggcatagagc accagcgcaa tcagtgcggg tgtcattccg
     8161 gttcctgcta tgccgagctt ccccagccac ggaaaggccg taaccagcgc ggcaagcggc
     8221 gcaatcaaca ggccaaagag cgccaccgaa ggcacggtct gaatgacatt gagcagagaa
     8281 aaaattgccc cctgccgcgc agtggaaaag tagcaccaga tgcccaacgg cacaccaatc
     8341 actaacgcag gcagcaccgc accaaacagc aacgtcagat gttgtgccag cgcgtcgtca
     8401 aacacatcct gacggttggc gtattctttc attagtgaga gatcgttaag cgtgccggag
     8461 tacagcaacc acagcggaat aatggcaatc tgcatatgca acaaccagcg ccacagcgga
     8521 tgcgtggaga ttcggcggat ggcatcgcta caggccagca atgccagcgc cgcagccagc
     8581 cagaaaccac tgccgaggct ggtacgcgcc agcgcactgc cattttgcgc cagttgggtc
     8641 gccgcctttc cagctcccca caccaacaat acgaagacga attgcgccag aatgagtgca
     8701 caaatgctgc cttttttagc ggggacaaaa caggctgtca gccaggcgca accaacgccc
     8761 accagcatcc agatcgtttg cggccacagt tgccagagat gacgcccttc acccgagact
     8821 aaacgattag gcgcgtagct gataaacggc agcgctgctg cgattgccgt cagcaacagc
     8881 agcagcgcca gaacaggatt aatacgtaga taagtcacgg gaaattactt cgcccaccct
     8941 ttttgtttca ggtagtcggc agccactttt ttggcatcca gtccttccac tgcaatgctg
     9001 gcattcagtt gctgcaatgt ttttgcatcg aggctggcga agactggctg tagccactgt
     9061 gccatttgcg gatactcctt caacaccgat tcacgcacca ctggtgcagg cgcatagata
     9121 ggttgcacgc cttgcggatc gcttaaggtt tgcagcccca gcgccgctac cgggccgtca
     9181 gtgccgtaag ccattgcagc attaacgcca gaggtttgct gggcagcggc tttgatcgtc
     9241 accgccgtgt cgccaccagc cagtgacagc aactgatcct gaccgagctt aaagccatag
     9301 gctttttcaa acgcgggtaa cgcatcggcg cgttcgataa actctgccga ggctgccagt
     9361 ttgaaggtgc cgccctcttg cagataacga ctcaggtcgg caagcgaagt gagtttattt
     9421 ttctctgcca catcctgacg cacggcgata gtccaggtgt tatttgcagg cgcgggcgtc
     9481 agccagatta acttgttgtg ctccgaatcg agctttttga ctttctcgta accttgctgc
     9541 gcgtttttcc acgctgcatc gttttcatct ttaaagaaga aagcgccatt gccggtatat
     9601 tccggataga tatccagttc accggaagta atcgccccgc gcaccacagg agtcgttccg
     9661 agttgcactt tattcaccgt cggtacgccg tggctttcca gcacctgcaa aatgatattg
     9721 ccgagtagcg caccttcggt atcgattttt gaaccgactt taacggggga agccgcctgt
     9781 agcggcaggc tcacggctgc caacataacc agtgaacctg cccagagctt taagagtggc
     9841 atgatgcttt cctcattctt ttactgttgt tttcagcgaa ttaagagaaa agcatagttg
     9901 ataatggcgg ggttagcttg agcggaagga ttcagttgca gaatcagata aatacttaag
     9961 aggcatattc ggtccggcgt ttcctgccgg atgcggcgcg agcgccttat ccggcctaca
    10021 aagggcgcaa acgtcgcgcc cttactaaag cattacagca actcaaactc gcctttctta
    10081 acgcgtgcgg aatcagtgcc gataaataca ttgaacttgc caggctcggc gtcatatttc
    10141 atctgttgat tccagaactt cagcgcctca atatcgatcg ggaagctgac agtctgagtt
    10201 tcgcccggtt tcagggtgat tttctcaaag cctttcagct gtttcacagg gcgactcatg
    10261 gaagccgtca catcctgcaa gtacatctgc actaccgtgg ccccctcgcg cttaccggtg
    10321 ttcgtcacct gcacgctggc agtcactttg ccgtcacgct tcatggtcgg cgcagaaagt
    10381 ttcacatcag agacggtgaa agtggtgtag ctcagcccat agccgaacgg atacaacgcc
    10441 ccgttagctt catcaaaata acgcgaagtg tatttgttcg gcttgtcggc attatacggg
    10501 cgaccggtat tcagatggct gtagtacacc gggatctgcc cgacagaacg cgggaaggac
    10561 attggcagct tgccggacgg gttgtaatcg ccaaacaata catcggcaat tgcattaccg
    10621 ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag cctgctgatc ttctttcacc
    10681 agcgccagcg gacgcccgtt catcagcacc agcaccagcg gtttaccggt ggctttcagc
    10741 gccgcaatca agtcacgttg gctttgcgga atagtgatat cggtccggct ggaggcttcg
    10801 tgcgccatcc cctgtgcttc accgactaca gccaccacca catcagattg tttcgccgtc
    10861 tgcaccgctt catcaatcat ctcttgcggc gaacgcggat cgactttgac cgcttcttca
    10921 tactgattca ggaaatcgat aatgcctttg tcactggtaa cgttcgcccc tttggcatac
    10981 agcactttac cgttttcacc gacggaattt ttaatcccgg tcagtacggt cacggattga
    11041 tcggcaacac cggctgcgga ccagctgccc atcacgtcac gtttactgtc cgccagtggc
    11101 ccaaccaccg caatggtggc cgattttttc agcggtaacg tttcgagacg gtttttcagc
    11161 aacaccaggc tttcgcgcgc cacttcacgc gcttctttac ggtgcaggcg gctttcggca
    11221 ttggtatcca ccgggtcaga ctctttcggc cccaaatggc tgtatgggtc gttaaacaat
    11281 cccatatcat atttaacgtt cagtacatgg cgggcagcat cgtccagctc ttccatcgtc
    11341 actttgccgg atttgatcaa cccaggcaga tacttcgagt aatactcgtc gctcatactc
    11401 atgttgattc cggatttcag cgccacgcgc accgcatctt ccgggtctgc cgccgtgcca
    11461 tgtttaatca gctctttgat tgcaccgtga tcggaaacgg tgatgccttt aaagccccac
    11521 tggtcgcgca gaacatcttt cagcagccag gaatcggagg tggctggcgt gccgttcagc
    11581 gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca gccccgcttt gtacggcggc
    11641 atataatcat taaacaggcg ctgcggactc atatcgacgg tgttgtactc tttaccgcct
    11701 tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca tcaccgagta gcgatctgcc
    11761 gggcttttac cctgcatcgc ttccaccatg gttttaccca ttgttgaggt gagatacgta
    11821 tcttcgccaa aaccttcgga agcacgtccc cagcgcggat cgcgcgagac atcgaccatc
    11881 ggtgcccagg tcatattcag gccatcatct gccgcttcat aagcagagac acgtccgacc
    11941 gttttcactg catcgaggtt aaaagacgag gccagaccga ggctaatcgg gaacaccgtg
    12001 cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa ttttcaggcg gcttaattcc
    12061 atcacctgat cctgcatggc gcggatatcc tgacgggtta cggtgttgaa aatcgcccca
    12121 acctgaccgt ctttgatcat ctcgcggatc gcctctttcg ggttatccgg gccgacgctg
    12181 attaagcgca gctgaccaat tttctcatca actgtcattt tcttaagcag ttcggtgacg
    12241 aacgcatccc gcgcttcggg cgttaatgga tggttgccga ataaatcatc cgccagtgct
    12301 ggctgcaggg ccagactcac cgcgattcct actgaacata gccatttcat atggattttt
    12361 cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa aaccgtagtt tgccataagc
    12421 atgatggaga gagaaaaaga atgctcagtt tattgtctga attttcaaaa tattcactcg
    12481 ctgaattgtc atacaaggcg ctattctagt ttgtgatatt ttttcgccac cacaaggagt
    12541 ggaaaatgtc ttccatgaca acaactgata ataaagcctt tttgaatgaa cttgcccgtc
    12601 tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac ggcccgctat cgcaagggct
    12661 tccgttctgg tcagggcgac gcgctggctg tcgttttccc tggctcacta ctggaattgt
    12721 ggcgggtgct gaaagcctgc gtcaccgccg acaaaattat tctgatgcag gctgccaata
    12781 caggcctgac cgaaggatcg acgccaaacg gtaacgacta tgatcgagat gtcgttatca
    12841 tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa gggcgaacag gtgctagcct
    12901 atccgggcac cacgctctat tcgctggaaa aagccctcaa accgctggga cgcgaaccgc
    12961 actcggtgat tggatcgtcg tgtataggcg catcggttat cggcggtatt tgtaacaact
    13021 ccggcggctc gctggtgcaa cgtggcccgg cgtataccga aatgtcgtta ttcgcgcgta
    13081 taaatgaaga cggcaaactg acgctggtga accatctggg gattgatctg ggcgaaacgc
    13141 cggagcagat ccttagcaag ctggatgatg atcgcatcaa agatgacgat gtgcgtcacg
    13201 atggtcgtca cgcccacgat tatgactatg tccaccgcgt tcgtgatatt gaagccgaca
    13261 cgcccgcacg ttataacgcc gatccggatc ggttatttga atcttctggt tgcgccggga
    13321 agctggccgt ctttgcggta cgtctagata ccttcgaagc ggaaaaaaat cagcaggtgt
    13381 tttatatcgg caccaaccag ccggaagtgc tgaccgaaat ccgccgtcat attctggcta
    13441 acttcgaaaa tctgccggtt gccggggaat atatgcaccg ggatatctac gatattgcgg
    13501 aaaaatacgg caaagacacc ttcctgatga ttgataagtt aggcaccgac aagatgccgt
    13561 tcttctttaa tctcaaggga cgcaccgatg cgatgctgga gaaagtgaaa ttcttccgtc
    13621 cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct gttccccagc catttaccgc
    13681 cgcgcatgaa aaactggcgc gataaatacg agcatcatct gctgttaaaa atggcgggcg
    13741 atggcgtggg cgaagccaaa tcgtggttgg tggattattt caaacaggcc gaaggcgatt
    13801 tctttgtctg tacgccggag gaaggcagca aagcgttttt acaccgtttc gccgctgcgg
    13861 gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt cgaagacatt ctggcgttgg
    13921 atatcgctct gcggcgtaac gacaccgagt ggtatgagca tttaccgccg gagatcgaca
    13981 gccagctggt gcacaagctc tattacggcc attttatgtg ctatgtcttc catcaggatt
    14041 acatagtgaa aaaaggcgtg gatgtgcatg cgttaaaaga acagatgctg gaactgctac
    14101 agcagcgcgg cgcgcaatac cctgccgagc ataacgtcgg tcatttgtat aaagcaccgg
    14161 agacgttgca gaagttctat cgcgagaacg atccgaccaa cagcatgaat ccgggaatcg
    14221 gtaaaaccag taaacggaaa aactggcagg aagtggagta aaaattacgg atggcagagt
    14281 atcgccatcc gaattcactt aatcgttctg tgccgtctgc cccgccgccg ccatttgggc
    14341 ggctttttgt tttttatagc tcaacgctgc tgccggcaca ggcatcactt taccggtttc
    14401 aatccaggta cgcaggcggc tggcatcggc aaaatgggta tatttgccaa acgcgtccat
    14461 cactaccagc gccaccggtt tattattgat aaccgtacgc atcaccagac aatggcccgc
    14521 cgcattggta aagccggttt tggttaactg aatattccag ttatcgcgat acaccagatg
    14581 attagtattg cggaacggca gcgtatacgt cgggttagag aaggttgcca tatcttcccg
    14641 ggtagtactt aactgcccga tcaacggata ctgtttgctg gcaatgagca gtttggttaa
    14701 gtcacgggca gttgaaacgt tatgcaccga caatccggta ggttcaacaa agcgcgtgtt
    14761 gttcattccg agcgatttcg ctttcgcatt cattgcctta ataaaggctt tgtaaccacc
    14821 gggataatgg tgcgcaaggc ttgccgccgc gcggttttct gaagacatca gcgccagcaa
    14881 cagcatatct ttacggctga tttcgctatt cagtcgtacg cgcgaataga cccctttcat
    14941 ctccggcgtc tggctgatat ccacttttag tttttcatcc agcggcagtc gtgcatccag
    15001 cacaaccatc gcggtcatta atttgctgat agacgcaatc ggacgcacca gatccgggtg
    15061 gttcgaatag atcactttgt tggtattcag atcaacaatc atcgcgctac cggaggcaat
    15121 ttccggttgt gaagcggtgg tagcggctgc cgttttcgca acggcctgcg gtgcaaaagg
    15181 cacagccagc atcagggcca ggctaaataa agaaactcga aatttcggca tgatgagcat
    15241 tcagatagtg gttcacgcgc acgggttgcg caccgccgga gtaaggattt actgaggcta
    15301 gcgacgccat cataacgagc aaaaagtgcg atcgtcaaag gagaatcgtg aggaaatgct
    15361 gcattgctga catttacgcc agcaatgcaa cgtcaaagaa ctttctagaa caaacgataa
    15421 ccgtagcccc atagtataac ggttagggcg agcagcactt ccagtaccag cacgccaatc
    15481 gccagcgtcg aactagagaa gctaaggcct tcctctttgt tgatattcag gaagctcgga
    15541 atatcaaggt aaagcaggta gccggtgtaa aacagcgcca ccgtgccgac cagcgcacac
    15601 aaccagacca gtggataaag cgccaccaga ccgcttaaaa acagcggagt tgcaacgtag
    15661 cccgcgaaga ccatgcagtg cgcaagtgac ggacgctgcg gataattacg cgccatccac
    15721 cagatgaccc gccccatcac cgcgacccca gccagcataa cgccgtaaaa caagacagcc
    15781 agcgccagtc cggtaaacca ggataacttc aggatagtgc catcgccaaa attccagcca
    15841 atctgtgtag tgccaatgaa ggcgcaaatc accggaatcg ccgccatcag caaaacgtgg
    15901 tgggtgtaat ggtgagaaat cgtttcgttt tcgcgattaa tcacctgcat ttcacgatcg
    15961 ggatgggaaa acagtcccca gacatggctc ataccgcccc cttgttgtga gttcatgaac
    16021 ctgacagttc aagtataagt cagcttgtga ttattttttg ttcgcccatg taattttcac
    16081 tgtctgatga acgtcctttt tcagcgtgaa tgattcacag ggtgtatgct tactggcaac
    16141 caaagggaga cagactggcc tatggatctc aatacactta tctcacaata tggttatgcc
    16201 gcgctggtga tcggtagcct ggcggaaggt gaaaccgtga ctttgctggg aggcgttgcg
    16261 gcgcatcagg ggctattaaa gttcccgctg gtggtacttt ctgtggcgct tggcggcatg
    16321 attggcgacc aggtgctcta tctgtgcggg cggcggtttg gcggcaagct gttacgccgt
    16381 ttctcgaaac atcaggataa aattgagcgg gcgcagaaac ttatccaacg ccatccgtat
    16441 ctgtttgtca ttggtacgcg ctttatgtat ggctttcggg tgattggccc gacgctgatt
    16501 ggtgccagcc agctgccgcc gaaaatcttt ctgccgctga atattctcgg cgcatttgcc
    16561 tgggcgttga tttttaccac tattggttac gctggtggtc aggtgattgc gccgtggttg
    16621 cacactctcg accagcattt gaagcactgg gtctggttga ttctggtcgt ggttctggtg
    16681 gtaggcgtgc gctggtggct gaaacgacgc gggaagaaaa agccggataa tcaggcgtaa
    16741 aaccattgcc ctggataagg cgttcgcgcc gcatccgaca tccggggcac catgcctgat
    16801 gcgacgcttg tgcgtcttat catgcctacc aaaattttac ccattgaaat aaccacgcac
    16861 ctcactgcgc tattctgggt tgaactgcgg attcgccaac ataaagccgc catccactat
    16921 caacgactgc ccggtggtgt aatttgcgcc ctctgagcac agccacgcta ccaggctggc
    16981 aatctcatgc gttgcgccaa aacgccgcag aggaatcgaa ggctccgcgt cgggcttcac
    17041 gtagctgtca tccatgccat tcattggcgt ggcgatcgcc ccaggcgcga ctgcgttcac
    17101 caaaatctta tgcctgacca gctccagcgc catcgttttg gttaacccgc cgagcgcatg
    17161 tttagcggct gtgtaagcgc tggcatccgg cagcggcgta tgttcatgta ccgacgtaat
    17221 gttgatgatg cgaccgccct gcccttgttt caccatctgg cgggccgcaa tttgtgagca
    17281 taaaaatgca ccatcgacat caacggtaaa aatcttgcgc cactcatcga aagccatatc
    17341 aagaaacggc gctttggtca ttgcgcccgc attattcacc agcacatcaa tacgccccag
    17401 ccgttgaatg agtttctcca gcgcctgtgc accttctggc agtttaccga gatccagctg
    17461 cacaatctcc gcacgtacgc cgtggctaac tacctcacgc gcggtatctt ttgccccttc
    17521 ttcatctgag tgccaggtaa tgccaatatc aaacccctgc tgcgccagta ataacgcgca
    17581 ctctttgccg atccccgaat cggaggcggt aataatcgca acctgtgcca tcgagttctc
    17641 cacttaacgc tgaataaacg ttaagtatag aaggcgcata tcatcagcgt ttgtaacccc
    17701 cgcccaacgc accagtgagt tgaatggaag catccagcca ctgcccttgc aataacaggc
    17761 cattggcccg ctcacgcagc gcggggattc tggcttcgct gacgcgggaa ccagcaatga
    17821 tgcccgcgtt aaagcgcgcc tgcgcaagac ctaccacacg caaggcatcg cgctcaattt
    17881 gcgcctgatg ctggtttttc tccgcaagtg tctgaacctg actggctgcc cgcgccacgt
    17941 cattcaccgc ttcaaccacc gctttgttgt agctggcgat agacaagttg ctttcggctt
    18001 ttgcgatatc gagattggcg ttaagacgac cactatcgaa aatgggtagc gtcaggcctg
    18061 ccgtaacgcc catttgctgc gcggaatgac ggaacagatc gcttaagtgc aacgcatcct
    18121 gttgcaggaa ggccatcagg ttgatgtcag gataaaatgc cgcttttgcc gcatcaatgg
    18181 tgcttagcga tgactcaacg taccagtgcg ccgcctgcaa atctgcccgc cgggccagta
    18241 aggagcaccc cagttcatca ggaagctggc ttgccacttt cggcaacgcg accggtttaa
    18301 gcttcaatga ctttgtctgg ttatttgtaa gtgcgcttaa ccgtgcctca ataattttca
    18361 ttttccccgc gacatcgttg agctgctgcc gggttttgct ggcattaata tcggtttcca
    18421 caccttcaac tgaagaagta atcccgttct gatatagctg gcgatcggtc gcgataatgg
    18481 tgttctgctc tttttctatt tgctgcaaga ccgtgtttaa cgccgcctgg gtttgccact
    18541 cccagtacag gcgggctacg ctgccagcca gcaattggcg ggtttgctcg cgttccgccg
    18601 cccgtgcttt aaccgtaccc aggcgggcag taacctccgc ccgattcttt ccccagatat
    18661 cgagatgcca gcccgccgtt aagccaaaag ttccgttggt gtaccacgga ccggtcgtac
    18721 ctgcggccgg atcgttcaga gcaaacggcc ccattaagcc ttctgccgac attttttgcc
    18781 gctccatatc cgcagaaaag tcgatctgcg gaccatcctg agtagcaatt gccttcgcct
    18841 gggcttcagc tagctgaatg cgctgttcag ccacctgcat atccggtgcg ttctgtagtg
    18901 cattgttaat taaggaagtg agttgattat cgtgatactc cagccaccat tggctgtctg
    18961 gccaaccatt tttcagcgcc gtgggtaatg cggtgtcaac ttgtgcagcg ggcgtttgct
    19021 ggcttaacgc ctggcgggtt tcatgcatag gcgcacaccc ggccagcatc agtaacagcg
    19081 gaaaacaggc gatggcggga taaaaggaat cacgattcat gggggaataa tcaggtaaga
    19141 aaaggtgcgc ggagattacc gtgtgttgcg atatattttt tagtttcgcg tggcaataca
    19201 tcagtagcaa taaaacgaca tatccagaaa aatatacact aagtgaatga tatcttccga
    19261 tttatcttaa tcgtttatgg ataacggcaa agggcttcgt tttttcctat acttattcag
    19321 cactcacaaa taaaggaacg ccaatgaaaa ttatactctg ggctgtattg attattttcc
    19381 tgattgggct actggtggtg actggcgtat ttaagatgat attttaaaat taattaatgt
    19441 catcaggtcc gaaaataacg agaatatttc agtctctcat cctgttgcgc tcctgtcatg
    19501 tgtattgctt catata
//
LOCUS       DAZGAN020000088        19377 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGAN020000088 DAZGAN020000000
VERSION     DAZGAN020000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.088"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..379)
                     /locus_tag="ACHKYO_RS17455"
                     /old_locus_tag="pgaptmp_003491"
     CDS             complement(<1..379)
                     /locus_tag="ACHKYO_RS17455"
                     /old_locus_tag="pgaptmp_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU4430270.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYIS"
     gene            745..2067
                     /gene="xylA"
                     /locus_tag="ACHKYO_RS17460"
                     /old_locus_tag="pgaptmp_003492"
     CDS             745..2067
                     /gene="xylA"
                     /locus_tag="ACHKYO_RS17460"
                     /old_locus_tag="pgaptmp_003492"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HGU4430271.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQHPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            2139..3593
                     /gene="xylB"
                     /locus_tag="ACHKYO_RS17465"
                     /old_locus_tag="pgaptmp_003493"
     CDS             2139..3593
                     /gene="xylB"
                     /locus_tag="ACHKYO_RS17465"
                     /old_locus_tag="pgaptmp_003493"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HGU4430272.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            3762..4103
                     /gene="yiaB"
                     /locus_tag="ACHKYO_RS17470"
                     /old_locus_tag="pgaptmp_003494"
     CDS             3762..4103
                     /gene="yiaB"
                     /locus_tag="ACHKYO_RS17470"
                     /old_locus_tag="pgaptmp_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HGU4430273.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            4149..4586
                     /gene="yiaA"
                     /locus_tag="ACHKYO_RS17475"
                     /old_locus_tag="pgaptmp_003495"
     CDS             4149..4586
                     /gene="yiaA"
                     /locus_tag="ACHKYO_RS17475"
                     /old_locus_tag="pgaptmp_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HGU4430274.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(4628..5623)
                     /gene="wecH"
                     /locus_tag="ACHKYO_RS17480"
                     /old_locus_tag="pgaptmp_003496"
     CDS             complement(4628..5623)
                     /gene="wecH"
                     /locus_tag="ACHKYO_RS17480"
                     /old_locus_tag="pgaptmp_003496"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HGU4430275.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            5798..6097
                     /gene="ysaB"
                     /locus_tag="ACHKYO_RS17485"
                     /old_locus_tag="pgaptmp_003497"
     CDS             5798..6097
                     /gene="ysaB"
                     /locus_tag="ACHKYO_RS17485"
                     /old_locus_tag="pgaptmp_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HGU4430276.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            6192..7103
                     /gene="glyQ"
                     /locus_tag="ACHKYO_RS17490"
                     /old_locus_tag="pgaptmp_003498"
     CDS             6192..7103
                     /gene="glyQ"
                     /locus_tag="ACHKYO_RS17490"
                     /old_locus_tag="pgaptmp_003498"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGU4430277.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            7113..9182
                     /gene="glyS"
                     /locus_tag="ACHKYO_RS17495"
                     /old_locus_tag="pgaptmp_003499"
     CDS             7113..9182
                     /gene="glyS"
                     /locus_tag="ACHKYO_RS17495"
                     /old_locus_tag="pgaptmp_003499"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGU4430278.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHCT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            9508..9660
                     /gene="hokA"
                     /locus_tag="ACHKYO_RS17500"
                     /old_locus_tag="pgaptmp_003500"
     CDS             9508..9660
                     /gene="hokA"
                     /locus_tag="ACHKYO_RS17500"
                     /old_locus_tag="pgaptmp_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HGU4430279.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(9649..9708)
                     /locus_tag="ACHKYO_RS17505"
                     /old_locus_tag="pgaptmp_003501"
     CDS             complement(9649..9708)
                     /locus_tag="ACHKYO_RS17505"
                     /old_locus_tag="pgaptmp_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430280.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(9848..10060)
                     /gene="cspA"
                     /locus_tag="ACHKYO_RS17510"
                     /old_locus_tag="pgaptmp_003502"
     CDS             complement(9848..10060)
                     /gene="cspA"
                     /locus_tag="ACHKYO_RS17510"
                     /old_locus_tag="pgaptmp_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HGU4430281.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(10341..10631)
                     /gene="yiaG"
                     /locus_tag="ACHKYO_RS17515"
                     /old_locus_tag="pgaptmp_003503"
     CDS             complement(10341..10631)
                     /gene="yiaG"
                     /locus_tag="ACHKYO_RS17515"
                     /old_locus_tag="pgaptmp_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HGU4430282.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            11065..11775
                     /gene="yiaF"
                     /locus_tag="ACHKYO_RS17520"
                     /old_locus_tag="pgaptmp_003504"
     CDS             11065..11775
                     /gene="yiaF"
                     /locus_tag="ACHKYO_RS17520"
                     /old_locus_tag="pgaptmp_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HGU4430283.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(11825..12799)
                     /gene="ghrB"
                     /locus_tag="ACHKYO_RS17525"
                     /old_locus_tag="pgaptmp_003505"
     CDS             complement(11825..12799)
                     /gene="ghrB"
                     /locus_tag="ACHKYO_RS17525"
                     /old_locus_tag="pgaptmp_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HGU4430284.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(12903..13562)
                     /gene="yiaD"
                     /locus_tag="ACHKYO_RS17530"
                     /old_locus_tag="pgaptmp_003506"
     CDS             complement(12903..13562)
                     /gene="yiaD"
                     /locus_tag="ACHKYO_RS17530"
                     /old_locus_tag="pgaptmp_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HGU4430285.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            13715..16048
                     /gene="bisC"
                     /locus_tag="ACHKYO_RS17535"
                     /old_locus_tag="pgaptmp_003507"
     CDS             13715..16048
                     /gene="bisC"
                     /locus_tag="ACHKYO_RS17535"
                     /old_locus_tag="pgaptmp_003507"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HGU4430286.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQERGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(16017..16457)
                     /gene="yiaC"
                     /locus_tag="ACHKYO_RS17540"
                     /old_locus_tag="pgaptmp_003508"
     CDS             complement(16017..16457)
                     /gene="yiaC"
                     /locus_tag="ACHKYO_RS17540"
                     /old_locus_tag="pgaptmp_003508"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU4430287.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(16454..17017)
                     /gene="tag"
                     /locus_tag="ACHKYO_RS17545"
                     /old_locus_tag="pgaptmp_003509"
     CDS             complement(16454..17017)
                     /gene="tag"
                     /locus_tag="ACHKYO_RS17545"
                     /old_locus_tag="pgaptmp_003509"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGU4430288.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFVDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            17175..17873
                     /gene="yhjY"
                     /locus_tag="ACHKYO_RS17550"
                     /old_locus_tag="pgaptmp_003510"
     CDS             17175..17873
                     /gene="yhjY"
                     /locus_tag="ACHKYO_RS17550"
                     /old_locus_tag="pgaptmp_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU4430289.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            18102..19304
                     /gene="yhjX"
                     /locus_tag="ACHKYO_RS17555"
                     /old_locus_tag="pgaptmp_003511"
     CDS             18102..19304
                     /gene="yhjX"
                     /locus_tag="ACHKYO_RS17555"
                     /old_locus_tag="pgaptmp_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4430290.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         4742 a         4640 c         5020 g         4975 t
ORIGIN      
        1 aagaaatata gaaatcgata tccgcatcgt taatcatacg atcgtaagct aatactttaa
       61 taccttcttg tttggcttct tttacaacgt tacttaatac ctgaccgtta tacggaataa
      121 tgacaagaac atcgacaccc cggtttatca tgttttcaat ctgcgacatt tgtgtttctt
      181 cattgccatt tgcagactgt acaaatactt tcgcgccgag agattctgcc tttttcacaa
      241 aaatatctcg atctttttgc cagcgttcaa gacggagatc atcaatcgcc atacctattt
      301 tgacttcttt ggcgtgtgcc gcaacgttgg taagcagaag tgaggtgcaa agggtgagta
      361 gaatgttctt tattttcatg gtgtagggcc ttctgtagtt agaggacagc tttaataagt
      421 aacaatcacc gcgataaacg taaccaattt ttagcaacta aacaggggaa aacaattaca
      481 gatttttatc tttcgattac gatttttggt ttatttcttg atttatgacc gagatcttac
      541 ttttgttgcg caattgtact tattgcattt ttctcttcga ggaattaccc agtttcatca
      601 ttccatttta ttttgcgagc gagcgcacac ttgtgaatta tctcaatagc agtgtgaaat
      661 aacataattg agcaactgaa agggagtgcc caatattacg acatcatcca tcacccgcgg
      721 cattacctga ttatggagtt caatatgcaa gcctattttg accagctcga tcgcgttcgt
      781 tatgaaggct caaaatcctc aaacccgtta gcattccgtc actacaatcc cgacgaactg
      841 gtgttgggca agcgtatgga agagcacttg cgttttgccg cctgctactg gcacacattc
      901 tgctggaacg gggcggacat gtttggtgtg ggggcgttta atcgtccgtg gcagcaccct
      961 ggtgaggcac tggcgttggc gaagcgtaaa gcagatgtcg catttgagtt tttccacaag
     1021 ttacatgtgc cattttattg cttccacgat gtggatgttt cccctgaggg cgcgtcgtta
     1081 aaagagtaca tcaataattt tgcgcaaatg gttgatgtcc tggcaggcaa gcaagaagag
     1141 agcggcgtga agctgctgtg gggaaccgct aactgcttta caaaccctcg ctatggcgcg
     1201 ggtgcggcga cgaacccaga tcctgaagtc ttcagttggg cggcaacgca agttgttaca
     1261 gcgatggaag caacccataa attgggcggt gaaaactatg tcctgtgggg cggtcgtgaa
     1321 ggttacgaaa cgctgttaaa taccgacttg cgtcaggagc gtgaacagct gggccgcttt
     1381 atgcagatgg tggttgagca taaacataaa atcggtttcc agggcacgtt gcttatcgaa
     1441 ccgaaaccgc aagaaccgac caaacatcaa tatgattacg atgccgcgac ggtctatggc
     1501 ttcctgaaac agtttggtct ggaaaaagag attaaactga acattgaagc taaccacgcg
     1561 acgctggcag gtcactcttt ccatcatgaa attgccaccg ccattgcgct tggcctgttc
     1621 ggttctgtcg acgccaaccg tggcgatgcg caactgggct gggacaccga ccagttcccg
     1681 aacagtgtgg aagagaatgc gctggtgatg tatgaaattc tcaaagcagg cggtttcacc
     1741 accggtggtc tgaacttcga tgccaaagta cgtcgtcaaa gtactgataa atatgatctg
     1801 ttttacggtc atatcggcgc gatggatacg atggcactgg cgctgaaaat tgcagcgcgc
     1861 atgattgaag atggcgagct ggataaacgc atcgcgcagc gttattccgg ctggaatagc
     1921 gaattgggcc agcaaatcct gaaaggccaa atgtcactgg cagatttagc caaatatgct
     1981 caggaacata atttgtctcc ggtgcatcag agtggtcgcc aggagcaact ggaaaatctg
     2041 gtaaatcatt atctgttcga caaataacgg ctaactgtgc agtccgttgg cccggttatc
     2101 ggtagcgata ccgggcattt ttttaaggaa cgatcgatat gtatatcggg atagatcttg
     2161 gcacctcggg cgtaaaagtt attttgctca acgagcaggg tgaggtggtt gcttcgcaaa
     2221 cggaaaagct gaccgtttcg cgcccgcatc cactctggtc ggaacaagac ccggaacagt
     2281 ggtggcaggc aactgatcgc gcaatgaaag ctctgggcga tcagcattct ctgcaggacg
     2341 ttaaagcatt gggtattgcc ggccagatgc atggagcaac cttactggat gctcaacaac
     2401 gggtattgcg ccctgccatt ttgtggaacg acgggcgctg tgcgcaagag tgcactttgc
     2461 tggaagcgag agttccgcaa tcacgagtga ttaccggcaa cctgatgatg cccggattta
     2521 ctgcgcctaa attgctatgg gttcagcggc atgagccgga gatattccgt caaatcgaca
     2581 aagtattatt accgaaagat tacttgcgtc tgcgtatgac gggggagttt gccagcgata
     2641 tgtctgacgc agctggcacc atgtggctgg atgtcgcaaa gcgtgactgg agtgacgtca
     2701 tgctgcaggc ttgcgactta tctcgtgacc agatgcccgc attatatgaa ggcagcgaaa
     2761 ttactggtgc tttgttacct gaagttgcga aagcgtgggg tatggcgacg gtgccagttg
     2821 tcgcaggcgg tggcgacaat gcagctggtg cagttggtgt gggaatggtt gatgctaatc
     2881 aggcaatgtt atcgctgggg acgtcggggg tctattttgc tgtcagcgaa gggttcttaa
     2941 gcaagccaga aagcgctgta cacagctttt gccatgcgct accgcagcgt tggcatttaa
     3001 tgtctgtaat gctgagtgca gcatcgtgtc tggattgggt cgcgaaatta accggcctgt
     3061 gcaatgtccc agctttaatc gctgcagctc aacaggctga tgaaagtgcc gagccagttt
     3121 ggtttctgcc ttatctttcc ggcgagcgta caccacacaa taatccccag gcgaaggggg
     3181 ttttctttgg tttgactcat caacatggcc ccaatgaact ggcgcgagca gtgctggaag
     3241 gcgtgggtta tgcgctggca gatggcatgg atgtcgtgca tgcctgcggc attaaaccgc
     3301 aaagtgttac gttgattggt ggcggggcgc gtagtgagta ctggcgtcag atgctggcgg
     3361 atatcagcgg tcagcagctc gattaccgta cgggggggga tgtggggcca gcactgggcg
     3421 cagcaaggct ggcgcagatt gcggcgaatc cagagaaatc gctcattgag ttgttgccgc
     3481 agctaccgtt agaacagtcg catctaccag atgcgcagcg ttatgccgct tatcagccac
     3541 gacgtgaaac gttccgtcgc ctctatcagc aacttctgcc attaatggcg taaacgttat
     3601 cccctgcctg accgggtggg ggataattca catctatata tctcactaat taattaatat
     3661 ttagtatgaa tttattctga aaatcatttg ttaatggcat ttttcagttt tgtctttcgt
     3721 tggttactcg taatgtatcg ctggtagata tggagatcgt tatgaaaacc tcaaaaactg
     3781 tggcaaaact attatttgtt gtcggggcgc tggtttatct ggttgggcta tggatctcat
     3841 gcccattgct aagtggaaaa ggctattttc ttggcgtgtt aatgacagca acttttggta
     3901 actatgcata tcttcgcgca gaaaaactcg ggcaactgga taattttttt acccatatct
     3961 gccagttagt tgcgttaatc actatcggtc tcttgtttat cggtgtttta aatgcaccta
     4021 tcaatgctta tgaaatggtg atctacccca tcgccttttt tgtctgcttg tttggtcaaa
     4081 tgcgtttgtt tcgctcggta tgagcaacat aaagctctta cacattcagg aatgaaagga
     4141 atactgtgat ggacaacaaa atatcaacct attcaccggc ctttagtatt gtgtcatgga
     4201 tagctctcgt tggtggtatc gttacctatc tgttagggct atggaatgca gagatgcagt
     4261 taaatgaaaa agggtattat tttgccgtac tggtgttagg actgttttct gctgcgtctt
     4321 atcaaaagac cgttcgggac aagtatgaag gcataccgac cacttccatt tattatatga
     4381 cctgcctgac tgtctttatt atctctgttg cattactgat ggtaggtctg tggaatgcga
     4441 cattattact cagtgaaaaa ggtttttatg gactggcttt cttcttaagc ttgtttggtg
     4501 cagtagcggt gcagaagaat attcgtgata ccggaataaa cccgccaaaa gaaacacagg
     4561 ttacccagga agaatacagc gaataattca cgtaagcccg gtcagtccaa tgtgaacggg
     4621 cttttactta actcactaat ctgtttctgt cgattcgttg taccagcata gaaagtaaca
     4681 aactcgctgc caacgtcgcg caaaagatcc aaataatatc cagtattggc caatttttaa
     4741 gctcaattcc ccgggtgcgc agcgcatgga taatcaaggc gtggaatccg tatataccca
     4801 atgaatggcg ggagattaag ccaagtccgc gaatggtacg cgtatccagc gtgtttttaa
     4861 ccagagtcaa tagcgcgatt gcgcagataa aaaccatcgg cccacagtaa agataccagg
     4921 tatcggcaaa atttccgcgc cactgcaatt catataatgt cccgcgagag ataataaaaa
     4981 cccccgtcgc aaacagcgcg gcgctcaccc acgacagtgc tttatgctgt gtgtccatca
     5041 tccctatagc gcggcccaac atgccataca gaatgtagta aaaagtatcg ccattgatat
     5101 ataagttaat tggcagccat tcaaaaccgt caattttctg cggcactgtg tttgggttag
     5161 cgataatgcc aatcaccacc attagcacca gcaacatttt tccgccgacg ttcttcacct
     5221 gaatcagcgg tgaaaccaga taaatcaccg caatcgcgaa gaaaaaccac aagtggtaaa
     5281 acactggctt ttgcagcagg tttttcagcg ctaactccat attgatggag gtaaacagcg
     5341 caatgtagag cagtgcgatt gcgctataaa aaatcagaca taagccgata cgcaagaaat
     5401 ggcgcgactg ggcgctgcgt tcgccaaaaa agagatagcc ggaaatcatg aaaaatagcg
     5461 gcacgctgac acgagaggca gaattcagaa cattggcgat atcccaggtg acggggctaa
     5521 cactatgagc attggtcaca taccaggtag tggtgtgaat catcaccacc attaaacacg
     5581 ctatccctcg caggttatca atccagtaaa ttttgggctg catctgtgtc tctgtatctg
     5641 gttaaaaaaa gtctgaccga taaatcattc agttggcgca ctggaataat ctgagtttta
     5701 tcactacagc ttatagaggc ttaaggaaat tcgtaagata tcagccacta taccgatata
     5761 aataataaga ctcacctgca aaccagacgg taatttaatg atgatgaacg ctttctttcc
     5821 ggcaatggcg cttatggtgc tagtgggttg ttctacaccg tcacccgtgc agaaagcaca
     5881 acgggtaaag gttgatcctc tgcgttcgtt gaatatggaa gcgttatgca aggatcaggc
     5941 ggcaaaacgt tataacaccg gcgagcaaaa aatcgacgtc accgccttcg aacagttcca
     6001 gggaagctat gaaatgcgcg gttatacctt ccgtaaagag cagtttgtct gttcttttga
     6061 cgcagatggc cattttttgc atctttccat gcgttaagcc ctgctttttc ccgtttcgta
     6121 ctgtatatct tccatccagc gggtatactg atcccttcct ttaaatccac acgtatccag
     6181 cacgaaataa tatgcaaaag tttgatacca ggaccttcca gggcttgatc ctgaccttac
     6241 aggattactg ggctcgccag ggctgcacca ttgttcaacc attggacatg gaagtcggcg
     6301 cgggaacctc tcacccaatg acctgtctgc gcgcgctggg gccagaaccg atggcggctg
     6361 cttatgttca gccttctcgt cgcccgaccg atggtcgcta cggcgaaaac cccaaccgtt
     6421 tacagcacta ctatcagttc caggtggtca ttaagccatc gccggacaat attcaggagc
     6481 tgtacctcgg ttctctgaaa gagctgggca tggacccgac tattcacgac atccgtttcg
     6541 tggaagataa ctgggaaaac ccgactctgg gtgcctgggg actgggctgg gaagtgtggc
     6601 taaacggcat ggaagtgacg cagttcactt acttccagca ggttggtggt ctggagtgta
     6661 aaccggttac cggcgagatc acctacggtc tggaacgtct ggccatgtac attcagggcg
     6721 tagacagcgt ttacgacctg gtctggagcg acggcccgct gggtaaaacc acctacggcg
     6781 acgtgttcca tcagaacgaa gtggagcagt ccacttacaa cttcgaatac gcggatgtgg
     6841 acttcctgtt cacctgcttt gagcagtacg agaaagaagc gcagcagctg ctggcgctgg
     6901 aaaatccgct gccgctgcca gcctacgagc gtattctgaa agccgcccac agcttcaacc
     6961 tgctggatgc gcgtaaagcc atctccgtca ccgagcgtca gcgctacatt ctgcgcattc
     7021 gcaccctgac caaagcagtg gcagaagcat actacgcttc ccgtgaagcc ctcggcttcc
     7081 cgatgtgcaa caaagataag taagaggcgg ctatgtctga gaaaactttt ctggtggaaa
     7141 tcggcactga agagctgcca ccaaaagcac tgcgcagcct ggctgagtcc tttgctgcga
     7201 attttactgc ggagctggat aacgctggcc tcgcacactg caccgttcaa tggtttgctg
     7261 ctccgcgtcg tctggcgctg aaagtagcta acctggcgga agcgcaaccg gatcgtgaaa
     7321 tcgaaaaacg cggcccggcg attgcccagg cgttcgacgc tgaaggcaaa ccgagcaaag
     7381 cggcagaagg ttgggcgcgt ggttgcggta ttaccgttga ccaggctgag cgtctgacta
     7441 ccgataaagg cgaatggctg ctgtatcgcg cccatgtgaa gggcgaaagc accgaagcac
     7501 tgctgccgaa tatggttgcg acttctctgg cgaaactgcc gatcccgaaa ctgatgcgtt
     7561 ggggcgcaag cgacgtgcac ttcgtgcgtc cggtgcacac cgtgaccctg ctgctgggcg
     7621 acaaagtcat tccggcaacc attctgggca ttcagtccga tcgcgtgatt cgcggccacc
     7681 gctttatggg cgaaccggaa ttcaccatcg acaacgccga tcagtatccg gaaattctgc
     7741 gtgagcgcgg gaaagtcatc gccgattacg aagaacgtaa agcgaagatt aaagccgatg
     7801 ccgaagaagc agcgcgtaag attggcggta acgctgactt aagcgaaagc ctgctggaag
     7861 aagtggcttc gctggtggag tggccggtcg ttctgaccgc aaaattcgaa gagaaattcc
     7921 tcgcggtgcc ggctgaagcc ctggtttaca ccatgaaagg tgaccagaaa tacttcccgg
     7981 tgtatgcgaa cgatggcaaa ctgctgccga acttcatctt cgttgccaac atcgaatcga
     8041 aagatccgca gcaaattatc tctggtaacg agaaagtcgt tcgtccacgt ctggcggatg
     8101 ccgagttctt cttcaatacc gaccgtaaaa aacgtctgga agataacctg ccgcgcctgc
     8161 aaaccgtgct gttccagcaa cagctgggta cgctgcgcga caaaactgac cgcatccagg
     8221 cgctggctgg ctggattgct gaacagattg gcgctgacgt taaccacgca acccgtgcgg
     8281 gcctgctgtc taagtgtgac ctgatgacca acatggtctt cgagttcacc gacacccagg
     8341 gcgttatggg gatgcactat gcgcgtcacg atggcgaagc ggaagatgtc gcggtggcgc
     8401 tgaatgagca gtatcagccg cgctttgccg gtgatgacct gccgtctaac ccggtagcct
     8461 gtgcgctggc gattgctgac aagatggata ctctggcggg tatcttcggt atcggccagc
     8521 atccgaaagg cgacaaagac ccgtttgcgc tgcgtcgtgc cgcacttggc gtgctgcgaa
     8581 ttatcgttga gaagaacctc aaccttgatc tgcaaacgct gaccgaagaa gcagtgcgtc
     8641 tgtatggcga taagctgact aacgccaacg tggttgatga tgttatcgac tttatgctcg
     8701 gtcgcttccg cgcctggtat caggacgaag gttacaccgt tgacaccatc caggcggtac
     8761 tggcgcgtcg tccgactcgt ccggctgatt tcgatgcccg catgaaagcg gtatcgcact
     8821 tccgtaccct tgaagcagct gctgcactgg cggcggcgaa caagcgtgtc tccaacattt
     8881 tggcgaaatc tgacgaagtg ctgagcgacc gcgtgaatgc ctctaccctg aaagagccgg
     8941 aagaaattaa actggcgatg caggttgtgg tgctacgtga caagctggag ccgtactttg
     9001 cggaaggtcg ttaccaggat gcgctggtcg aactggcaga gctgcgtgag ccggttgatg
     9061 ctttcttcga taaagtgatg gtcatggttg atgacaaaga attgcgtctc aaccgtctga
     9121 ccatgctgga gaaactgcgc gaactgttcc tgcgcgttgc ggatatttcg ctgttgcagt
     9181 aataacgccg ttattaaaaa gcctgccatc tggcaggctt tttttattat cgctaaataa
     9241 tacagcaacc tttaataatc ttctgctgaa taaagattat ctcatatatt aattttatga
     9301 ggttttttta ggattatatc aaggagaaga aacaaactta ttaagctaga atagccacgg
     9361 gtgcttgaga ctgtttgtct caggtattca ccgaaaggca gacagagaaa agccccacct
     9421 gactataaat caaagtgagg ctaccctatg cctgaacacc ataaggttag cctcttactc
     9481 gttggaaatc aacacagggg gctgggaatg ccgcagaaat atagattact ttctttaata
     9541 gtgatttgtt tcacgctttt atttttcacc tggatgataa gagattcact gtgtgaattg
     9601 catattaaac aggggagtta tgagctggcg gcatttttag cctgcaattt aaaagagtaa
     9661 gagtcttcgg cgggaagtta ttcccgcctt tcttacggcg ttgcgcattc tcattgcacc
     9721 caaatttatt tttcacaaaa ataatactag attttattac gcgatcgatt atttatttcc
     9781 tgaaaaacaa ataaaaaaat ccccgccaaa tggcagggat cttagattct gtgcttttaa
     9841 gcagagatta caggctggtt acgttaccag ctgccgggcc tttagcgccg ctttcgatgg
     9901 tgaaggacac tttctgacct tcgtccagag atttgtaacc atcgttctgg atagcagaga
     9961 agtgtacgaa cacgtcttta gagccatcgt caggagtgat gaagccgaag cctttgtcag
    10021 cgttgaacca ttttacgata ccagtcattt taccggacat agtgtattac ctttaataat
    10081 taagtgtgcc tttcggcgat atggcgtgct ttacagattt tgaagcgtta aaggaatgtg
    10141 cactacgagg ggtatcaacg ataactcttg aagggacttg ccttactaca ctgctttaat
    10201 ggtctgtacg tcaaaccgtt gatgtgcatt aagccacgca ttggcgggtg atgcaacaat
    10261 tatttttcat atttatgatt aatcggttgg aaaacggtcc tgtcatcagg accgtaaaca
    10321 gcaataaagt ggataaaagt ctattccatc aactgcttac ttaatgccgg gttggcttga
    10381 atcaaacgca tcaattttag ttcggcactt gaaggcttca cgcgtctgga ttcccattcc
    10441 tttaccatgg cgactgatac gcccaaaacc cgggcgaaat catcgatttt taatcctgtc
    10501 ccttttcgta actgctcaat ttcggtacag gacgttgttc tgtgcgtcag ggtaattttc
    10561 tgcgtttcat ctttaaaaac aatctgttcc aggctgctca acagctcatg cattggatct
    10621 ttatattcca ttgaaaactc ctcaaatcac actgcgggat cgtgaattgc atcgaagctc
    10681 attaagaata gtcgggaaaa gcagacacag cggggtgaag tcagggcatg ctctggctat
    10741 gattcgattc agccagtcat ttagcgtgac ttatagccac ctaacgatct gataaatgcc
    10801 gatatatctc ttaccactcc tggtttgtaa agatttcgaa aatctaacct tctgcgaagg
    10861 cgtaaatctc tggctacaaa aaaagcaacg gcactatcat tttttaacaa tgaaacaaca
    10921 aattaacctt gtttcatttt ctccatgatt ttaccaggaa gattacgtag aaagggtatc
    10981 ctgcaagcct gtcctggact atccttgtca cgtcagacac gcgtgtgtcg ttgtgcgctt
    11041 tttttgggtg aaaggagtaa gaaaatggcg acaggaaagt cctgctctcg ctggtttgcg
    11101 cctcttgcgg cgttattaat ggtagttagc ctgagtgggt gttttgataa agaaggcgat
    11161 cagcgtaaag cgtttatcga cttcctgcag aatacagtga tgcgtagcgg tgaacgtcta
    11221 ccaaccctga ctgccgatca gaaaaaacag tttggtcctt ttgtctctga ttacgcgatt
    11281 ctgtatggtt attctcagca ggtgaatcag gcgatggatt ccggtctgcg cccggtcgta
    11341 gacagcgtta acgcaattcg cgtaccgcag gattacgtta cgcaaagcgg tccgctgcgt
    11401 gaaatgaacg gttcgctagg tgtgctggcg caacaactgc aaaatgcgaa gttgcaggcc
    11461 gatgctgcgc actctgcgct aaaacagagc gatgacctga aaccggtctt tgatcaggca
    11521 ttcaccaaag tggtaacgac gccagctgat gcattgcaac cgttgattcc ggcggcacaa
    11581 actttcacgc aacaactggt gatggttgga gactacattg ctcagcaagg tactcaggtg
    11641 agctttgtcg caaatggcat ccagttcccg acttcacagc aggcaagtga atataacaaa
    11701 ctgattgcgc cattaccggc acagcatcag gcgtttaatc aggcctggac tacagcagtt
    11761 actgcaaccc aataaagagt aaaagcccga gcgattatcg ggctttactc tacgcagtcg
    11821 cggcttagtc cgcgacgtgc ggattcacac agttcttctc aacctttcct tgtaacgcat
    11881 caatcaaatt atccacggca caggcggcca tgccataacg cgtctcatgg gtggcagatc
    11941 caatatgcgg tactgcgacg acgttggcca ttgagagcaa cggcgaatct acggacagtg
    12001 gctcttgttc gaagacatcc agcccggcgg cgtgaatttc ccctttctgc aatgctgcga
    12061 tcagtgcatt ttcgtcaacc accgggccac gtccggcatt aatgaaaatg gcggaggatt
    12121 tcattttggc gaattgttct gcgccaaaca gatgatgcgt ctcatcagtt aacggcagga
    12181 tcaggcaaac gaaatctgac tcttgtaaca gtgtatccaa atcgcagtag cgggcgttga
    12241 agcgttcttc tgcttcttta tggtggcggc gcgcgttata gaggatgggc atgttgaagc
    12301 caaagtgcgc acgttgtgcc agcgccatac cgatccgtcc catcccgaca atgcccagtg
    12361 ttttatggtg aacgtcagtg ccgtaccagt ccgggcctat gctcgcggtc cattcgcctg
    12421 cttttacccg ttctgctacc tccacaaccc gacgagcggt agacaacacc agcgccatca
    12481 gcgtatcggc gacggtttct gttaatacgg ttggcgtgtg catcagcaga atttttcggg
    12541 cggtaagcgc atcgacatca aaattgtcat agccgacgga gatcgttgat gtggcacgca
    12601 gtttcggcat tttttccagc aatgcggcat caacattctc gtttgaaccc agtaaacctt
    12661 cagcttcggc aaaaattgct gcattttgtt cgacggtttg tgggctgagg tttgccacct
    12721 ggtgaacggt gaaatgctct tgcaggcgtt gcagtaaatc atcaggtaag gctttgtaga
    12781 ggataacgga cggcttcatg cttctctcca ttcactgata aggcgatcac cttagcctga
    12841 aatatgatga aggaaaactg aagaatgcca ggtgatttct gcatcacctg gcatgaaagg
    12901 gattacagcg ggcttaaggt aatttctaca cggcggtttt gcgccttacc ttctgcggtg
    12961 ctgttgctgg cgattgggtt agccgggcca aggccctgag tacggatgcg gctggcgtcc
    13021 acgccctggg tgatcaacgc gctggcaacg gaatccgcac gttgctggga gagacgcatg
    13081 ttcaggtcgt gaccacccgt gctgtcggta taaccaatca cgttaaccgc cgttttcgga
    13141 tactctttca gcaccattgc cacgccggtc agggtgttag cgcctgccgg tttcagggtc
    13201 gcgctgctgc tgtcgaaggt cacattgttc ggcatattga ggataatgtt atccccgctg
    13261 cgggttacgc taacaccagt gccgcgcatt ttgtcacgca gcttcgcttc ctgcacatcc
    13321 atgtaataac ccacgccgcc gcccagagct gcgcctgctg ctgcgccaat cagcgcgcct
    13381 ttaccgcgat ctttcttcga agaagagagc gcaccaatac ccgcgcccac gagagagccc
    13441 agacctgcgc cgatagcaga tttgcctgct tcgcgttcgc cggtgtaagg gttagttgtg
    13501 cagccagata ccgccagagc gccactcact acggcggcaa taagataaac acgtttcttc
    13561 attgttaatc cttaataacc tttttattct ttgccacggg ttccgtggcg ggagattatg
    13621 ccgcgtgaac atgaagatta ttcctgggaa tactcggaaa tttgtaagta atatttaact
    13681 gctcaataca tctaaccttt caggagtctt cggtttggcc aactcatcct cacgatattc
    13741 cgttctgact gccgcccact gggggcccat gctggttgaa accgacggcg aaaccgtgtt
    13801 tagctcgcgt ggcgcgttag ccacaggaat ggaaaactcc ttgcagagcg cggttcgcga
    13861 ccaggttcac agcaatacgc gggtacgatt tccaatggtg cgcaaaggct ttctcgcgtc
    13921 accggaaaac ccgcaaggca ttcgtgggca ggatgaattt gttcgcgtga gttgggatga
    13981 ggcgctggat cttattcacc aacaacataa acgcattcgt gaggcttatg gtccggcatc
    14041 gatttttgct ggttcctacg gctggcgttc aaacggcgtg ctgcataagg cctcgacatt
    14101 attacaacgc tatatggcgc tggcaggcgg ttataccggg catctggggg attattcgac
    14161 cggcgcggca caggcgatca tgccgtatgt cgtgggtggt agcgaagttt atcaacagca
    14221 gaccagttgg ccgctggtgc tggaacatag cgatgtcgtg gtgctgtgga gtgctaatcc
    14281 actcaatacg ctgaaaattg cgtggaatgc atccgatgag caggggcttt cttacttttc
    14341 tgcactgcgt gacagtggga aaaagctgat ctgcattgat ccaatgcgat cggaaaccgt
    14401 cgatttcttt ggcgataaaa tggagtgggt ggcaccgcac atgggcaccg atgttgcgct
    14461 gatgctgggg atcgcccata cgctggtgga aaatggttgg cacgacgaag cgtttctggc
    14521 gcgttgcacc acaggttatg ccgtcttcgc ctcttatttg ctgggcgaga gtgacggaat
    14581 agcgaaaacc gccgaatggg cagcagagat ttgtggtgtt ggcgcagcga aaatccgcga
    14641 gctggcggct attttccacc aaaataccac catgctgatg gcaggctggg gaatgcagcg
    14701 ccaacagttt ggtgagcaaa aacactggat gatcgtcacg ctggcagcaa tgttggggca
    14761 aatcggcaca cccggcggcg gttttggtct ttcttaccat tttgccaatg gtggtaaccc
    14821 cacgcggcgt tctgcggtgc tctcttccat gcagggcagc ttgccgggtg gctgcgatgc
    14881 ggtagataaa atccctgtgg cccgcattgt tgaagcactg gaaaaccctg gtggcgcata
    14941 tcaacacaac ggtatggacc gacattttcc ggatattcgt tttatctggt gggcgggcgg
    15001 tgctaacttt actcatcatc aggataccaa tcgtctgatc cgtgcctggc aaaaaccgga
    15061 gctggtggtg atctctgaat gcttctggac ggcggcggcg aaacacgcgg atatcgttct
    15121 gcctgcgact acctcttttg agcgtaatga tctcaccatg accggtgatt acagtaatca
    15181 gcatctggtg ccgatgaagc aagtggtgcc gccacgctat gaagcgcgta atgattttga
    15241 tgtttttgcc gagttgagtg aacgttggga gaagggcggc tatgcgcggt ttacggaagg
    15301 aaaaagtcag ctgcaatggc tggaaacgtt ttataacgtt gcccggcagc gcggggcaag
    15361 ccagcaggtt gaattgccgc catttgctga gttctggcaa gccaaccagt taattgagat
    15421 gccggaaaac ccggacagcg agcggtttat tcgcttcgct gatttttgcc gcgatccgct
    15481 ggcgcatccg ttaaaaaccg ccagcggcaa gattgaaatc ttctcacagc gtattgccga
    15541 ttacggttac ccggattgcc ctgggcatcc aatgtggctg gagccggacg aatggcaggg
    15601 caatgccgaa ccagaacagt tgcaggtact ttctgcccat ccggcgcacc gcctgcacag
    15661 ccagctgaat tacagttctc tgcgcgaatt gtacgcggtg gcaaatcgtg agcctgtcac
    15721 cattcatcct gacgatgccc aggagcgcgg catacaagat ggcgatactg ttcggttgtg
    15781 gaacgcacgc gggcaaattc ttgccggagc ggtcattagc gagggaatta aacctggcgt
    15841 gatttgcatt cacgaagggg catggccgga tctggattta accgctgacg gtatttgtaa
    15901 aaacggcgca gtgaacgtgc tgaccaaaga tctccccagc tcgcggctgg ggaatggctg
    15961 tgcgggtaat acggcgctgg catggctgga aaaatacaac ggtccggaac tgacacttac
    16021 agcgtttgaa ccaccggcca gctcataatc catgtgggta gttgggtttc atcctgccat
    16081 gcgcaatcga caatgtgaaa accctgtgcc tggtaaaaat ttatcgccgg ttgatttttt
    16141 tgataaacct ccagcatcag gtggggatgg cgctgctgca catactgcat cagcgcctta
    16201 ccaataccgc gcctgacggc cttcggtgcg acaaacatcg ctgccagaaa tcggccttcc
    16261 ataatgctga caaaaccgag aagcttaccg tcttcttccc agacccagtt ttgcgcgttg
    16321 gcaagatagg catcccgcac cagcggaatg cagtcacgcc agtaattcgc ttttataaag
    16381 ggatgccccc aggttgtact ttccagccac agttcgagga tcgcggggag ttctgaacgt
    16441 tgcgcttccc gaatcatggt ttatttcccg gatagcaaca gcagccaacc acatgatcat
    16501 tcaccagccc acatgcctgc ataaaggagt aacagattgt ggtgccgaca aacttaaaac
    16561 cacgtttttt cagtgcctta gatagggcgt cggaggcgga cgtagatgtg ggaatttcgc
    16621 tcaacgttgt ggcttgtgtc acctgtggct gatgatttac aaacgaccag acaaagtcga
    16681 caaacggttc gccgttctgt tccatttgca ggtacgcccg cgcattacca ataattgcct
    16741 gaattttccc tcgatggcgg ataatcccgg cgtcctgtac cagtctttcg acatcctctt
    16801 cctgcattgc tgcgaccttc accggatcga actgatgaaa gcaggcgcga tagttttcgc
    16861 gttttttgag gacggtgatc cacgataacc cagcctgctg cccttcaagg cagatcattt
    16921 cgaacagttt tttactgtca gtttcaggca cgccccactc attatcatgg taggcaatat
    16981 aaagcgggtc ctgactcacc cagccgcaac gttccatact ttccctcgct atgtacccga
    17041 tcgtaaaaat ttcagccgct tttcttgacg catcggtgaa aaagtcaata tttaatacag
    17101 ggctactatg cccgatatcc ttcatacaat gacaataata ttgccgaatt cggctcttcc
    17161 ctggagtcgt gttaatgatc ataaaaaaaa gcggtggtcg ctggcagcta agcctgctgg
    17221 cgagcgtggt aatcagtgcc ttttttctca acacagctta cgcctggcaa caagaatata
    17281 tcgttgatac gcaacccgga cattccacag agcgttacac ctgggatagt gatcatcaac
    17341 ctgattacaa cgatattttg tcgcaacgta ttcaaagtag ccaaagggcg ctgggactgg
    17401 aagtcaatct ggcggaagaa actcctgtgg atgtgaccag cagtatgagt atgggctgga
    17461 attttccttt atatgaacag gttacaaccg gcccggtcgc ggcattacat tacgatggca
    17521 caaccacctc gatgtataac gagtttggcg acagtactac cacgctgacc gatccgttat
    17581 ggcatgccag cgtgagtagc ttaggctggc gtgttgactc ccggcttggc gatctccgac
    17641 cctgggcgca aatcagctat aaccagcaat ttggcgagaa tatctggaag gcgcaatcag
    17701 gcctgagccg gatgacggcg acaaaccaga acggcaactg gctggatgtc accgtaggcg
    17761 ctgatatgtt gctcaatcaa aatattgccg cctatgccgc gctatctcag gcagaaaata
    17821 ccactaacaa tagcgactat ctgtatacga tgggggttag tgccagattt taacgtaaaa
    17881 atcacaattg aaaccattgg ataacaacag ttgtggcgat agtgggtgct aacttactaa
    17941 ataataaatt tggtgaataa ttgtcgcgtc attcattcct gaactaaggc atttcattcc
    18001 gttctgatgg catttcatgc cgtttttccc caggcataaa atgcacttcg ttatggttgt
    18061 cggcagagat ttttcctttt tattactgca ggaatactgc catgacacct tcaaattatc
    18121 agcgtacccg ctggctgaca ctcatcggta ctatcattac ccagtttgca ctggggtcgg
    18181 tttatacctg gagcctgttt aatggcgcgc tttctgccaa gctggatgcg ccggtaagcc
    18241 aggtcgcttt ctctttcggc ttgttaagtc tggggctggc aatttcgtct tctgttgcgg
    18301 gcaaattgca ggaacgtttt ggcgttaaac gcgtcaccat ggcttccggc attttgctgg
    18361 gattaggctt cttcctgacc gcgcattcca acaacctgat gatgctgtgg ttaagcgccg
    18421 gtgtgctggt cggtctggcg gatggcgcgg gttacctgct gacgctctct aactgcgtga
    18481 agtggttccc ggagcgtaaa ggtctgatct ccgcgttcgc tatcggttct tatggtctgg
    18541 gtagcctggg tttcaaattt atcgacacgc agctgctcga aacggtgggt ttggaaaaaa
    18601 catttgtgat ttggggagcg attgcgctgg tgatgattgt tttcggcgca acgttaatga
    18661 aagacgcacc aaaacaggaa gtgaaaacca gcaatggtgt ggtggagaaa gattacacgc
    18721 tggcagagtc gatgcgtaaa ccgcagtact ggatgttagc ggtaatgttc ctgactgcct
    18781 gcatgagcgg cctgtacgtg attggggtag cgaaagatat cgcccaaagt ctggcacacc
    18841 ttgatgtggt ttccgcagcc aatgcagtaa cggttatttc catcgctaac ctttcaggtc
    18901 gtctggtgct ggggatactg tctgacaaaa ttgcccgtat ccgtgttatc accattggtc
    18961 aggtgatttc gctggtgggt atggcggccc tgctgtttgc accattaaat gcagtgacgt
    19021 tctttgcagc gattgcctgc gtggcattta actttggcgg cactattacc gtctttccgt
    19081 cactggtcag tgagttcttc ggcctcaata acctggcgaa aaactacggt gtgatttacc
    19141 tcggtttcgg tatcggtagc atttgtggtt cgattatcgc ctcactgttt ggcggcttct
    19201 atgtgacctt ctacgtgatt ttcgccctgc tgattctgtc attggcgctt tctacgacaa
    19261 ttcgtcagcc agagcagaaa gtattgcgtg aggcgcatat ttagaaatat gttgctactg
    19321 aaataaatgc gaggagggag acttcaaagt ctcccttttt ttatggacgc atattaa
//
LOCUS       DAZGAN020000089        19150 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGAN020000089 DAZGAN020000000
VERSION     DAZGAN020000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.089"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(65..538)
                     /locus_tag="ACHKYO_RS17560"
                     /old_locus_tag="pgaptmp_003512"
     CDS             complement(65..538)
                     /locus_tag="ACHKYO_RS17560"
                     /old_locus_tag="pgaptmp_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4430291.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(672..872)
                     /locus_tag="ACHKYO_RS17565"
                     /old_locus_tag="pgaptmp_003513"
     CDS             complement(672..872)
                     /locus_tag="ACHKYO_RS17565"
                     /old_locus_tag="pgaptmp_003513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430292.1"
                     /translation="MKHIPEGVIQAPVSPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNG"
     gene            complement(872..1693)
                     /locus_tag="ACHKYO_RS17570"
                     /old_locus_tag="pgaptmp_003514"
     CDS             complement(872..1693)
                     /locus_tag="ACHKYO_RS17570"
                     /old_locus_tag="pgaptmp_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4430293.1"
                     /translation="MRLVSRFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVQKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(1871..2002)
                     /locus_tag="ACHKYO_RS17575"
                     /old_locus_tag="pgaptmp_003515"
     CDS             complement(1871..2002)
                     /locus_tag="ACHKYO_RS17575"
                     /old_locus_tag="pgaptmp_003515"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGU4430294.1"
                     /translation="MAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            complement(2096..2557)
                     /locus_tag="ACHKYO_RS17580"
                     /old_locus_tag="pgaptmp_003516"
     CDS             complement(2096..2557)
                     /locus_tag="ACHKYO_RS17580"
                     /old_locus_tag="pgaptmp_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HGU4430295.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKMLTDP"
     gene            complement(2633..5149)
                     /locus_tag="ACHKYO_RS17585"
                     /old_locus_tag="pgaptmp_003517"
     CDS             complement(2633..5149)
                     /locus_tag="ACHKYO_RS17585"
                     /old_locus_tag="pgaptmp_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430296.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLNGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLVLVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVAGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEALLPELPA"
     gene            complement(5270..8392)
                     /gene="agn43"
                     /locus_tag="ACHKYO_RS17590"
                     /old_locus_tag="pgaptmp_003518"
                     /gene_synonym="flu"
     CDS             complement(5270..8392)
                     /gene="agn43"
                     /locus_tag="ACHKYO_RS17590"
                     /old_locus_tag="pgaptmp_003518"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HGU4430297.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WINAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSSAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGSLA
                     GTTTLNNGATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGRVEKSGSGTLTVSNTTL
                     IQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNML
                     QAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGIY
                     GAAGHSSVDVKDDDASRAGTVRDDAGSLGGYLNLTHTSSGLWADIVALGTRHSMKAST
                     DNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGYG
                     SAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKHGVSELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGY
                     NGQATLNVTF"
     gene            complement(8726..9598)
                     /locus_tag="ACHKYO_RS17595"
                     /old_locus_tag="pgaptmp_003519"
     CDS             complement(8726..9598)
                     /locus_tag="ACHKYO_RS17595"
                     /old_locus_tag="pgaptmp_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4430298.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            10865..12355
                     /locus_tag="ACHKYO_RS17600"
                     /old_locus_tag="pgaptmp_003520"
     CDS             10865..12355
                     /locus_tag="ACHKYO_RS17600"
                     /old_locus_tag="pgaptmp_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HGU4430299.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(12477..13049)
                     /locus_tag="ACHKYO_RS17605"
                     /old_locus_tag="pgaptmp_003521"
     CDS             complement(12477..13049)
                     /locus_tag="ACHKYO_RS17605"
                     /old_locus_tag="pgaptmp_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430300.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(13135..13341)
                     /locus_tag="ACHKYO_RS17610"
                     /old_locus_tag="pgaptmp_003522"
     CDS             complement(13135..13341)
                     /locus_tag="ACHKYO_RS17610"
                     /old_locus_tag="pgaptmp_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4430301.1"
                     /translation="MNTPVSLMADQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
     gene            14198..14422
                     /gene="hha"
                     /locus_tag="ACHKYO_RS17615"
                     /old_locus_tag="pgaptmp_003523"
     CDS             14198..14422
                     /gene="hha"
                     /locus_tag="ACHKYO_RS17615"
                     /old_locus_tag="pgaptmp_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HGU4430302.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVITRTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            complement(14754..14951)
                     /locus_tag="ACHKYO_RS17620"
                     /old_locus_tag="pgaptmp_003524"
     CDS             complement(14754..14951)
                     /locus_tag="ACHKYO_RS17620"
                     /old_locus_tag="pgaptmp_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430303.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
     gene            15150..15719
                     /locus_tag="ACHKYO_RS17625"
                     /old_locus_tag="pgaptmp_003525"
     CDS             15150..15719
                     /locus_tag="ACHKYO_RS17625"
                     /old_locus_tag="pgaptmp_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HGU4430304.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGELNFGCSRS"
     gene            15980..16363
                     /locus_tag="ACHKYO_RS17630"
                     /old_locus_tag="pgaptmp_003526"
     CDS             15980..16363
                     /locus_tag="ACHKYO_RS17630"
                     /old_locus_tag="pgaptmp_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4430305.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGHECPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGRFSEEGGAE"
     gene            16360..16773
                     /locus_tag="ACHKYO_RS17635"
                     /old_locus_tag="pgaptmp_003527"
     CDS             16360..16773
                     /locus_tag="ACHKYO_RS17635"
                     /old_locus_tag="pgaptmp_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4430306.1"
                     /translation="MNGLKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCERLF
                     HGKKFCEACGTGIYCRAVCTKSVYRYQPDLLRNPE"
     gene            16779..16958
                     /locus_tag="ACHKYO_RS17640"
                     /old_locus_tag="pgaptmp_003528"
     CDS             16779..16958
                     /locus_tag="ACHKYO_RS17640"
                     /old_locus_tag="pgaptmp_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430307.1"
                     /translation="MQHNCLITPDTPLPDPDVVRRFVLRTVVQWLCLLMLLFMRHSDR
                     GDIDKHHLRANSYTE"
     gene            17079..17702
                     /locus_tag="ACHKYO_RS17645"
                     /old_locus_tag="pgaptmp_003529"
     CDS             17079..17702
                     /locus_tag="ACHKYO_RS17645"
                     /old_locus_tag="pgaptmp_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4430308.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYYL"
     gene            17791..18024
                     /locus_tag="ACHKYO_RS17650"
                     /old_locus_tag="pgaptmp_003530"
     CDS             17791..18024
                     /locus_tag="ACHKYO_RS17650"
                     /old_locus_tag="pgaptmp_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430309.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(18044..18253)
                     /locus_tag="ACHKYO_RS17655"
                     /old_locus_tag="pgaptmp_003531"
     CDS             complement(18044..18253)
                     /locus_tag="ACHKYO_RS17655"
                     /old_locus_tag="pgaptmp_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4430310.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPRKEPPAGNHTA"
     gene            18752..>19150
                     /gene="tssB"
                     /locus_tag="ACHKYO_RS17660"
                     /old_locus_tag="pgaptmp_003532"
     CDS             18752..>19150
                     /gene="tssB"
                     /locus_tag="ACHKYO_RS17660"
                     /old_locus_tag="pgaptmp_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HGU4430311.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGTEESISLTFRNMNDFT
                     PELVARQIPRLKAMLAMRSLLRDLKANLLDN"
BASE COUNT         4493 a         4836 c         5065 g         4756 t
ORIGIN      
        1 ctgcgctcgc cgggcgtcat cgcgccgggc agaaaggaaa gttgttgcat tgtacttctc
       61 ctgctcagtc gatgatgcgc ataatggcgc tgcattccgg atgctgcagg gcgtaatccc
      121 gcagccggta ataatggacc gtcatggcat aacactccgt acgacaggcg tgatgactgt
      181 acgtcatcag acaggcggca atgccggcgg cttccgggct catttcagcg cggttaccgt
      241 tcatggcatt gaacagtacc catgtttcgt catcatcgtc atccggttcg ggtgccataa
      301 atgccccgcc gttgttcagg gtatacagat tccagatacc accacagtag tcttcgcaaa
      361 gacggtccat ccagccgaaa acacggggct ccagggtcac ccactgtgga atgaggccaa
      421 aatgctgcgg ccagaagctg atgcgctgtt catcagggac tatggtggca accagttgtt
      481 gcaggccagt ctccggtgca caggctgaag aggtggtgtt ctgagacaat gttttcatgt
      541 gtgaaatcct tgtaatgtga ataaatgaga ggaaataaag gccgggggct gacaacatca
      601 cgttcatcac tgaccgtgag acccggcaat atcagggatg aatgatattc ggctgaaatt
      661 ccggacagcc gttaaccgtt ccggtcacag acggatggcc agatgccatt gcctgtactc
      721 agtctgcaca gactgagttc tccgggacgg taaagcagac gggtacacca ggcgcacagg
      781 tcatcatcag aaatggaacg acatcccggc agcgcctccg gggcgtcggg tgatacgggt
      841 gcctgaatca cgccttcagg gatatgtttc atcagcgcat gctctccagc agcgtttctg
      901 ccatcaccca caatgcgcgg ttgagcttaa tatcggtgtc gatgctgtga atggcacggg
      961 tgtggatacg ttttcctttt gcactgcgac cggaaatccc gcctttcagc atattctcct
     1021 ggatggtctg atatgcgctc cacaggtctt taccgtaatc ctcccgacgt cgtggcgtca
     1081 gaatgtcggc ggtggtgacg ggctgatgtt cgtcaccata acggtaagtc agtgccgcct
     1141 gtgccagcgc ctggcgtgcc ggtggcggca ggaccagcga ctgcatggca tcacgtttct
     1201 cttcaatccg gtcaaaaacg cccaccacct cgtaagcccc ttcaatgact ttctgcacca
     1261 catttccccg gtgtggaaca cgcacttccc ccagagactg accacagacg cacccgttct
     1321 ggcagacgaa ccggaagtaa cccggcagca tctggtagct ggaggtaccg tcatgggagt
     1381 tgagcagaat aatttcaggg acatgttctc cgtttatctc tccggcccgc cgcagacgca
     1441 gcatgtgttt tgtgtatccc cggcggcccg ggtcgcgcac acgggtctgg caggcgaaaa
     1501 agggctggaa gccttcccgc tgcagacttt ccagtacggt gatggtgggg atgtacgtat
     1561 agttctggct tcgggatgtg tgtttctctt ccccgaaaat ccccggcaca tggtgcatca
     1621 gctcttcatg tgtcagcgga cggtcacggc gtatctggtt tgcagcataa ccaaaacgac
     1681 tgactaatcg catactttgc tccttatcgg tggttaagat ttactggtgc aataaatgaa
     1741 aaagccgcgt ctcccggaga agacgcggcc tgatggacga aatcaatgac gtttattgtc
     1801 tgagaagccc tccactggcg agccacgcat taagctgttt tccggcatca gcttcagagt
     1861 tccggcagcg tcagcgtcgc tgaccttccg cgttgcggat aacccgacgg aaatgcattc
     1921 ccgggtcatc ctggataaac acattgcggt aagcggttat gacagcaacc gcctgcccgt
     1981 atgagaaaga tccttcagcc aggacatact ctgtgtgtaa cccggcatat ctggtttctc
     2041 ctgataaata gcctctgcca tacgttgtgg cagaggctga agcatgaaac tgacttcagg
     2101 gatcagttaa catttttccg gaaacggtaa tcagcagtgg atggtagtcc tggggatcga
     2161 aaaccgataa cggcagactg acacgatggc cgttactttc ttcagttgct ttaatgattt
     2221 cggttgtggc gacattttcc acgcactccg tttccagaaa tgcgtctgtg gttcgcgtgg
     2281 cattactgtc accaaaggct tccgtttcca tttttctggt caccagcgtc tgaccatatt
     2341 tgtctttgag ttgcagagtg atggtgaggg ggccaaatcc ttcatcgttt ccgccattat
     2401 ccagccggaa ctggtaagca caaatatttc ccgggagcca tatcgtatct gtattgcgta
     2461 tactgatgta acgttgatcc tgtgcccgga gtggggcaga ccacgttaac cccagaatga
     2521 aggcggtaat catgcaggtt ttgaacaggt gaatcatggt atttacctct ctgagtcatg
     2581 acgatgacac tgacaaatca ggtgataaaa cgtaaaaggc gcagaatagc cgttatgccg
     2641 gtaactccgg gagtaatgct tcttccagtc ggttaaccat cttgccgaga tgggatgcat
     2701 catattccat gacggggcgt tgcctgatga tactgaccac cagtggtttg attaacatat
     2761 tggtcgcggc ccgttgttgt ataccggcgg cgaaaatgat ctccagagca ttctgatcga
     2821 taacacgggc cagattcgcg ttcagatttt caatctcatg tccggtcagc aggaaactgc
     2881 cggcaagcca tgtcacctgc tcctgaatca ttgccagaat tttgacgcga tcttcttcaa
     2941 ccagcgctcc ggcaattttt cctgatacag cggaggcaat cattcccccc agaacaccgc
     3001 cagccacacg ccctgtcagt gcaccaaatg gtcccagtgg actgaacagt atcccgcccg
     3061 ccactgaccc gacagcacca cctgccacac cggaagatgc cacggcaaga ttcctgatga
     3121 actgcgcacc ggagatccgt ccccgcaaca ttttgatcat gtcagggccg gttgtgacag
     3181 cgaccagtac cagagatgtc accagcgtac ccttcatcac tttgttcagg gcactgatat
     3241 ttcctgcacc ggtccccttc tgaagcgcct gccggacagt cgggctggcc gtcgaaaaat
     3301 caatatgctt cagcataccc tgaacaacac tgagccggtg gagttgctgg gttgtgacgt
     3361 agacagccag tgtgcgggtg aaggtttttc ctgcctggac agcagctgtc tgcagggcag
     3421 catcgcgatc gccggtgctg agccagaaga ccgatgccgt cagggcaaaa ctgatccctc
     3481 cggccgccag actgacaacc gaaccctcgg caatatcata agtgaccgat tcgatggtcc
     3541 cgaaccgggt gatattacgg gcctgagtat aagtcagatg tccccgacga atcagccggg
     3601 acgcttctgc gggatcggtt acaccgggta ctttaccttc gcggatctta ttcctcatgg
     3661 tttcaacagc gccggcatac tgatcacggg ggacttccag ttgcatgggg ccattatttc
     3721 ccatgtaacg atactgtccg ttttgtccgt cgaatgccgc accgacgcta cgggctgcag
     3781 ttgaacaata tttcgtctgg atttctgttc cgctgaccag tctgtctgct ccatttctgg
     3841 cattattatc accgaggatc cgtgcgtttt tcagacggac tctgtcgaca atatggtttc
     3901 cccgttcagc ggcaaaccca tgtccctgcc ctccggaagc aaacattttc tcatttgtac
     3961 tccaggtttc cggcagtttc atgagaatca gaccattcgc cgttgcatcg gaaataaagc
     4021 tgttgtaagg gcgttcataa atttcctgac acagtgtttc tgtttcgcga caaagagagg
     4081 agcagtacca ttccggatcc ggcttatgat tttccggggc atgatgtact tctgtcagcc
     4141 cgcaggaggc acagatacga agctcatcca cgtgatggaa tgatacaaaa cggcgacctt
     4201 ctgaaaaggg ataccagtca ccgccgttca ggcaacgatc gagaaaatcg tcattcgctt
     4261 ccggtgataa attcaggcgg ttccggaaga gcgttttcag ttcatcaggt gtgaatgaca
     4321 ggttctcttt caactgttcg ttaaaccctg ccatgtttct ttcataccag gcattgttca
     4381 gtaaccagtt tccgtctgcc agttcaaatt ccctcagctc tccccgggaa atccgttgag
     4441 aaatgagcgt cagccagatg cgctccgtca gtgagtcagg aaggctgtaa tcgttttgca
     4501 aactggtcag ccacgattta ctgttgagca ccggttgttc ttccagtgtc tggctgatgg
     4561 cacaggtcag ttttttcagc gcctggatct ggctggccgc ccatagcagc gttactaatg
     4621 cagccagcca gtacaacaac ccgccccacc acatcagtga cggtgaaata tcgtactgta
     4681 acgcgatcaa tggtgcgcat gcagaacacg ccataaggaa aaaggcagta taaaaacagc
     4741 agtaataccc gttaatttcc ttgctgatat cttctggtgt gatcccgttc tgctgaatgt
     4801 ttttgatctg caggggaaca gccagcagac caccggcgag agcggtggtc agaataaatt
     4861 tcacagagtc aaaggtgtga gcctccagcg cgatatgttt accatcaagt ccccacatga
     4921 agctgatgat cattccggca atcgtcagaa atacgcctgc cagccagcga atatggaaca
     4981 accgggttat gcgttcggtg atatagccag ccagtaaggc cgggccgaac agacacagca
     5041 atatgagccc tgccagcgca gaagccgtgc tgattaatgc tctgaacaaa aggcgaagaa
     5101 tggcaaatcc tgcgatcagc aggatcagcg cgccgactat ctgtaacata gtcactccgt
     5161 gatgaatatc atgttattgt taatgccagc gaatgtggcg ttgaagatcc gttgcgccgg
     5221 tccgggtccc ggtcatgatg accgggacca cagagaggcg atggttctgt cagaaggtca
     5281 cattcagcgt ggcctgaccg ttataccctt cagcgctgct gccgctgacg ctgtgggcat
     5341 aaccggcctg aacgcccagg gtgatatttt cccggacacg ggcttccagt ccggcctgca
     5401 ggtccagtga cgtgccattc cgggacggtg agaacgtcat gttactgccg gctgcagctg
     5461 tacccatgct catgtccccc cgggagctga aggtgcggat aacagaaggt tgtacccacc
     5521 agttcacagg cagttcactc acaccgtgtt ttgtactgtc gcgcagggtg tcacgggatg
     5581 aggtgccttc accaaaggtc atatcgttgt ggctgcccag acggaaaccg gcacgcacat
     5641 gttgtgcact gccatacccg aacttcacat aaccggcgtt atcctggccg tcatccaggg
     5701 agagtccctg ccaggtgtac tgcagttgcg gctccagcat cagattgtca gtgatactga
     5761 agggcagacc ggtttccagt gaacccagcc agccccaccc ccgggcgcgg aagtcgttat
     5821 tgtccgttga cgctttcatg ctgtggcggg ttcccagggc cacaatgtca gcccacaggc
     5881 cggaggatgt atgtgtcaga ttcaggtatc cgccaaggct gccggcatca tcccggaccg
     5941 tgccggcacg ggaggcgtca tcatccttaa catcaacgga agaatggcct gcagcaccat
     6001 atatccctgc ggtcacagac ataccggcaa cctctgttct cagcaggtca ccctccaggc
     6061 ggacgaagcc atagctgccg ctgctttccg gcgtggcccc acgggcaata ccgccgttgt
     6121 tatcgtgacc gagatggccg ccctgaatgc tgagacggac gctgttattt tcaccgttta
     6181 caccggtctg atggctgcgg gagcctgcca gaatccggtc atagtccatt gcctgtgtca
     6241 gcatggaggc gtacaggggg acttcagcac gataacgttc ttcactgcgc agataccagc
     6301 tctcatcact gtcccggttg agggtgtagt taaaggcccc ggcctgcagc atattcccct
     6361 ggacaaaagc cccttcctcc gtggtggcac cgttaatggc ctcaaccacc tgaataccct
     6421 taccggtggt cgccagcccc gtaccactgt tgccggcgtt caccagattc aggatggttt
     6481 ttccggttgc cctgccaccg tcaatgacca gtctgtcagc attgttctgc gccatatccg
     6541 ggcgtacacg caggctgatg gtgccattct gcccgttcag gtttttcacc tgcagggttg
     6601 tcggaacgaa cttccctgtg cgggcggagg tgaaatgaat ctgtccggca tggctgaggt
     6661 catccaccac cgactgaacc gtggcgttat cggggatatt ccaggtggca ccggatgtca
     6721 gggtgacatt cgtggggtca atggcaccgt tcagcactgt gctgccggtc agcttcaggg
     6781 ccgtgccgcg ctgagcgatg acatccgtgg tgacggtact gtcgttcagc gtcagcgtgc
     6841 cttcattcag gttgacggtt ttctggatga gtgtggtgtt gctgacagtg agtgtgccac
     6901 ttcctgattt ttccaccctg ccgttaccgg taagagcgcc tccctgcagg agtgcatcac
     6961 cttcatggat ggtcagggta tcgttattca ccgttttccc ggaaagggta agcgtggcac
     7021 cgttattcag cgtggtggtg cccgccagcg agccccctct ggcggtgaac agtccgccat
     7081 ttaccgtggt atccgtggcc gtatcaccgg cgttcagtgt gaaggaactg cctttttcca
     7141 gcatcagggc atccgcctga ccgcctccga tacggaatgc cgttccgtcg ctccgggtac
     7201 cactgacagc ggcaccggaa tcggccagca gtacaccgcc attccccatg gatacggtgg
     7261 tggcggcacc accgttgcgg acatccagcg ttccgccatc atccacacgg gtattagtgg
     7321 ctgtgtgtcc gctcagcaca tccagacgtc cgccattttc cagtacgaca ttatcagcct
     7381 tacccgccac aacggagaat gctcccaggc ggtttgtacc ggtgacggtt gcagctgaac
     7441 tggtaaccag tgcaccgccc gtgttctggg tgacatcaga cgctttaccg ccggcattca
     7501 cctgcagctt tcctttctgg ttgatggtgg tatgcgcggc agttcctcct tccttaatca
     7561 cctgccagcc atcacggttt atcagcgtct ctgttgccgt gccaccgttg tgtacatact
     7621 ggttaccccc ctccagccgg gtgttatgtg cctctccgtg taccgtctgg tcaccaccgg
     7681 cgtaaatgag ggtgtcccgt gccatccccg aagaccagat aacctgccgg ccatttttgt
     7741 tgatggtggt ggattcagcc gtccctccga ccatctgacc gctggacaca ttttcagact
     7801 ccggaccacc ttctgcaccg gtgttgacga tggttccggt tgccagtccg ccatgtttta
     7861 tggtctggta accatcctgg ttaataattg taccggcagc tttccctccc ccgtgtaccc
     7921 actgctcacc tctgttatcc agcgtggtgt tcaccgccag tccgttaagg ctctgccctc
     7981 cgccatcacg aataaccgta tcactggcag ttcctcctgc ctgcacaatc tgacgaccat
     8041 ttgcggtgac agtggtgttt ctgcctgtgc cacccgcgtt tatccattgc ccgccggtgt
     8101 tttcgtcact gtccggcccc agctcaagcc cggtactgac agtcacgcca tcagctgttc
     8161 cggatacaaa ctggttgtca tggtttacca gtgttccgcc attcactgtt tcacccgggt
     8221 gcacaacgat gtcagcagcc agcaccggga gtgacgtgac cgcggcaaga gacagtgcaa
     8281 ccgccacacc gccacgttta ccccgtgcgc gggccagttc ggaggcaacc acgaaagcgc
     8341 ccgtaatgtg attccatacc agcctgtagc aggtattcag atgtcgtttc atcagcattt
     8401 ccttaatcag agtgagggtg aataaaaaag ccggtatcca cagatgtgga taccggcagg
     8461 taaagggtta cagtgccaga ggtccggctg acggaagttt ctgaattgtg gttctgttgt
     8521 tccctgaatc tcatgtctgt gtgtatctgc gcacgttgct aacaggagtt attattagca
     8581 taacgatcga tattaacgat cggtgaaata ggtatcgatc gttttattct attgtcaagc
     8641 tattgcacgg acaagagaga cagtaacgaa aacagcgctt ttgtgagcag acccagaggc
     8701 agggaggaca tcatccacga caggttcaga agaaaatcca gttccatacc gcacgggcaa
     8761 cagagaccac cgtgtcacga acagcgcgca gggccgtacg gacaacagag gcaacacaga
     8821 cgctctccgc tgtgtcaaat atccggtcca ccgcaccggt aaactgttca cgggcctgag
     8881 agcggacaga ttccgtgcgc agcccgtcct gcagtcgggt catcagggga ctggcggcat
     8941 ggtcgggaag cgctgtcatg agcgcactga ccagcgtatc cagttcccag ccggtgcggg
     9001 ccgatacggc cacaaccgga tgtacgggcc ggaacagacg gaataccgcc tccgttttct
     9061 cccgaatgtt ctgtgcctgt gcgggagaag gctgaatacc ggccatatcc cattcatggc
     9121 agggctccgt tttatcggcc tgcgtcacca caaacagtac ccgctgatgt ccccggtgca
     9181 gtatgtgtcg ccagaaatac tcatccacag acagggcacg gtcatcggct ttaatcagcc
     9241 acagtaccag gtccagttct ggcagaatgt cacggtacag ggcctcatac tctgcatccc
     9301 tgtcccggct ctcgcccacc ccgggcaggt cagtgataac catgctgtga ccgtgaccac
     9361 tcagacggaa gcgctgcact tcccgggtgc cggcgtgaac atcactgacc ggtgtgacct
     9421 ccccctgaaa cagtgcatta cagagtgaag atttaccggc cccgctttta cccataatgc
     9481 caatcacggg ttcgtgacgg gtgagtttgc gcagatgttc caggatgtga cgtgaaagcg
     9541 agtaaggcag ggaagaaagg ggtttttcaa ttgcctcaat ggcatcagac ggattcatac
     9601 atgtattccc ggtgaaaaaa gacaaaaccc cgcgcaccgg aaaggtggcg ggggatccac
     9661 ggagataata tctgtctgaa attgtttgga atcgatgggg atgttgcgta ttaacatgaa
     9721 ggagggcagg agttatccgt tactgggtga tatccttcca tgaaaatacg cggttattgt
     9781 taaacagcag gctgattact ccgtaaagga gaaaaatatt catcttgcag cctgattatc
     9841 aggtgtgttt atgtttactg aggtgagtat ttttcagtat attgtcgggg cagtcagtgg
     9901 gagatattaa atttacgttt agagttatct tgcttctgct gtgtattttt ataagagtgc
     9961 gcacgtaatg atatctgttg ataccatgtt gttaagtgtt tcccttcctg gtcatgcgta
    10021 atgattaaat gcttttgtga caccattctt ccggcatgaa tatttatcca gttccctttt
    10081 ttatttgtgt agtgatactg tcggaatagc gttcagtaat gtgaagtgtc ctgttaaaaa
    10141 tctttaataa atgtgtcatc ctggtcagga aatatctgct gagatattat tagttggtgg
    10201 tgtttttact gagtaatagc tggcttttaa ttcctgaact gaaatactgt cgctcgtcag
    10261 ggtttaatta aaactggatg atgatgtgaa aatgaaggct ggtttttgtg tttgtcgaag
    10321 aagtgttgtt tttatccatt ctcatcattg gctggcgatt atacagaagt atttgaagaa
    10381 aaaatggtgc attttatctg tgccggcagg gagggggatt ttcctgatat gtcagtatta
    10441 aatggttttt gttatttata aaaatgtcat cctgtccata gaatattgta ctgagtactc
    10501 agcattcagc gcaggaactg tcatcttctt tattttattc ttcttcagga gggctgtttt
    10561 ctgaaagcgt tactccgggg aaactgttta tgtttattcc cggcaggaaa cccgtacctg
    10621 caataccctg gatgatgctt tttgctggta cgttgttgtg tgaaaattaa tcaacatgtg
    10681 tgctccggag agcgcccgta gacgcacaga ttcattctga cattctttag cgacaaaata
    10741 tcgagtggtt attatttaac ctgaaaatcg acagaaattt ttatcctgta ttgattctgc
    10801 tgctattttt gctattagta gcaatagttg cggtacttta tgttctgtgt gttgtgaggt
    10861 cattatgcca tactttcagt atcctgatga gtttcccctg agctcgctgc ctccactcat
    10921 tcgtgatgcc gttattgaag ctcagcaaat cacgcaggct cccctggggc ttgtggcagc
    10981 atctgccctg ggcgctgttt cacttgtctg ccagaatctg attgatgtct gcagactgaa
    11041 tacactacgg gggccggttt ctcttttctt actgacgctt gctgagtccg gagaacgaaa
    11101 aaccgcagtg gacaaattat taatggagcc cctgtaccag caggagatgc tgttatattc
    11161 cagacacaaa aatgaactga cgacatggaa aaataaggaa gagcttttaa aggcgcagaa
    11221 aaaagcattg ttgtcaaaac tgaataaaga actccgcaaa ggcgcggatg agtcagaaac
    11281 gctcagacaa ctggaagcgc ttcagaaaaa cagaggggaa aaacctgtaa gatataaatt
    11341 catttttaat gatgccacga cagcagcaat taaagatcag ctttgtggtc agtggcgttc
    11401 agtgggtatc atgtctgatg aagccggtat aatatttgat gggtataccc tgtctgagct
    11461 gccttttatt aataaaatgt gggatggctc cgtattgtcg gtggacagaa agaatgaacc
    11521 cgaacagatg attgaaaatg ccagaatgac attatccctc atggtacaac ccggtctttt
    11581 tgaccgatat atggagcgaa aaggttctgt tgccagggac agtggttttc ttgcccgttg
    11641 tctaatcagt aaacctgcca ccacgcaggg gaaacgcttt attaacggag ctgtaacccc
    11701 gggaggatca ttaacggcat ttcatgaacg attaatggaa ctggccagag gcagtattga
    11761 aaaatccagt aaggatgaac gttattgtct gcatttttct cctgaagcgc aaaaaatatt
    11821 tattgatcac tataatgttc tggagcagga tttgtctcct tccggaccct taagccagtt
    11881 tcgggggcat gtttcaaaaa aaacagaaaa cattgcgaga attgctgcgt tattgcagta
    11941 tttcagtcag ggtgaaggga aaatctctgc cgatatcatg acgtctgctg tcgtaataag
    12001 ttcgtggtac acagatgaat ataaaaaatt atttgcgtta cctgatgaat ctgaactgca
    12061 gctgcaggac gcccgggagc tgctcgactg gctcattgag gagtgccgcg gagaatgccc
    12121 gccccgtgtc aggaaaaatt atattctcca gtgtggcccg ggccggttca ggaacaggaa
    12181 aaagctgaac gtcctgctga acattcttgc atctcaactc aggctctctg tcgtgcagga
    12241 aggaaaaacc acctatgtcc tgctttccga aataaacaat atcacgctgc ctgaactgaa
    12301 tgcgtgctac agccagaaac tgataagatg gcatgagcaa tcctttggct tataatgttc
    12361 cctgttgctg agacggatta ttcagtggtg gtatcgtagt atgcccgtca ccccccgctt
    12421 ctgcatgagg gtgacggagg aaaagtaagt gtgcggaggc ccgggagcgt tatgaatcaa
    12481 caggcgtata acaggtactc gtcaatcatc agttccagct cgcgcttttc ccgtttgcgc
    12541 gccacagccc agttttcaag ctctgcatgt gatttatgct ttaactcttc agtccagggc
    12601 tgcatggagt gggcacacat cagatcatac cagcacagcc atatcagcgt gtggtagaga
    12661 tcacgatcgt cgaaacgccg gagcaggctg ttcagcctgt ttgccagccg gttttcaccc
    12721 agacgatgta gctcactgag taaaacgccg cccagattaa aatgtgtaag cagcatgatt
    12781 tcatgggtct gaatgctggt cattaaccct tcctgaatgg caggaatgaa cggtggtgtt
    12841 cccgggttga cgacctgata gagtccctgt ggtgccactg agatgatagt gcctttttct
    12901 ggcagaaaaa agtgtgccag ggtcaggcgg tgataaccat gaagatgcgg agaccccatt
    12961 ccatgagcga cgataagcct cgtcacgctt gtcatccatt tctgatgctg gatgagcagc
    13021 agctcacacc ggttttcaag tttttgcata atattctctc ctttagcgcg cagcaaaatg
    13081 cctccggggg ccggatcatg cctttgctgc ggttgaacgg ataacggtca ggaattacgg
    13141 acgggactgt gcaatacgcg cctgcagcca ggtttccact tcacttttca gccagcggga
    13201 actgcggccc agtttgatgg gggcaggaaa ggcaccatcc ttgatgagct tgtaaaacca
    13261 tttatcggtc aggccggtca gttgagtgat aaatgccatg tcgaccatct ggtcagccat
    13321 cagtgaaact ggggtattca tgaaaatgtt cctccggatg ttgttaatct caaccggcgt
    13381 tggtgatgaa aacaacgcac caccttcacc ggcataacaa ccggggagaa acgtaaaaaa
    13441 taatcgggaa tgtgaccgtc aggagataaa cggggggatt gcggtggctg agccgtaccg
    13501 gtatgtcggg tgattgttgc gaaacgatgc ttccgcacgc gcacgggagg actttcgtga
    13561 gacaagggga aaactggcgt tctgataatt gcagagttgt ttttgaaggc ggttgtggtg
    13621 catcagcctg tgacctgtgg gcaggcgaca ggtatcactg gtgggataag actgtctgat
    13681 tggtattata aatatataag agagctcaaa cacatgagtt catatatgtg aaataaatct
    13741 gtgagtgtga tcagcagacc agtctaccca gtagcaacag attgcgttgt actcacaggg
    13801 ttatccctga taactggcgt ataacctgcg tacataaaac gtacctgcaa tgcacctgaa
    13861 cagagtgcat taagcatccg cctgagcggt aaacgtatcc gatctggtga gtgacagtct
    13921 gagagcattc tcagtaatcg cccccttgta tcctggaaat tggttaagag cgattagatt
    13981 tggcaaggtg tatgcatcca gcagcttaac acctcagcta actggctggt taccttcagg
    14041 ttgtcgcaat ggcatcggac atccggtgtc ggataactaa acgtagcgtt aaggcgatat
    14101 tgctgagaga ccggttaagt ggtggcacgg gagtaatgag tttggggagg gtaccagaat
    14161 gccagtcagg tgcggtatgc tggcagtatc acgttgattg cagcataaag taacgagaga
    14221 cgaatggaag ttaaaaccag agcagtttcg tcgttgttca tcccgggaga cgctggaaaa
    14281 agtgattacc cgcacacgtt ataaacttac ccctgcggag ctggaagcct ttaactctgc
    14341 ggtcgatcac cggctggcag aactgacaat gaacaaactt tacgatcgcg tgccggcttc
    14401 cgtctggaaa tatgtcacct gaatcttgaa tccgcgctac gatgactctg gtgttatctg
    14461 tgcaccggaa gatcgggaac attaagcagt ataacggcaa caaagaattc gaaatcaaaa
    14521 gggaatggtg tacagagggg caggcgttca ggcattaccg gcctgtatct tctgaagggg
    14581 actcgtattt gtcggggctg agtattccgc tcgattcgat cacggattgg cagaaacctg
    14641 actgtgattg attcaggtac cattttgcag ggatccggtg cgttactgtc tggcacaaca
    14701 aacgcagcgg gtcgctctgc cccgccgatt cctgataatt cctcccctgt aacctactgc
    14761 aacagtacaa atacactgac gcctgaaacg gtaaccagaa tcagcaccag cagtaacgtc
    14821 agtaaacaga gcagacgatc ggtcttccgg taataaccga aggggtcgac agcgccacaa
    14881 tacgggcaac acactgtatt atgagtaatt gcgtttccac agtccctgca cttctttttg
    14941 cgtatcacga tcgctccctc tccacgcctt atccgtaacc tgttttttac attaaaaaaa
    15001 caacgccggg aattcaatcg tcagtccgga gacggccgtt cgggttatca cagagtgcct
    15061 gagacagtgt cctgccggag gtcacctcag gggctctgcg cattttttac gacgtggcta
    15121 ttccgtatga accatacgga gatttaacca tgacctacaa atacaacccc ttctggcagc
    15181 aacgtattcg tgagacggtg cggcacgcac tggatgttca tccccgcctg acggcacttc
    15241 gggttgatct gcgtctcccg gatgtgccgg cagcaacgga cgcggctgtg atatcccgct
    15301 tcatcaatgc cctgaaagcc cgaatcgacg cttatcagaa acgtaagcat cgggaaggta
    15361 aacgcgtgca ttcctcgacc ctgcattacg cctgggcccg ggagttcggg gagcttaaag
    15421 gcaaaaaaca ctatcacctg ctgctgctgg tcaaccggga tacctggtgt cgtgccggcg
    15481 attaccgcgc tccggggtca ctggccggga tgattaaaca ggcctggtgc agtgccctgg
    15541 gagtggatgc cggacgttat gacacgctgg cacattttcc tgacaagcca gcggtgtggc
    15601 tggaacgcga tgatgaaacc ggctttcagc aggtgctgga acgttctggc tatctggcga
    15661 aggagcatac caaagctcgc ggcaccggtg agctcaattt tggctgtagc cggagttgaa
    15721 cacgacaggc gcaccaatac ccggcaaacc aaaagtatgg gttgttgtct ggcggcaacg
    15781 atgtatccac tcgcccactg actgtttttt acgtcagtgg gccattgtgt gacaaccaca
    15841 gtgctgtaac agcaaccagc ctgctttacc caaactccag tccagatttt aactgaaccg
    15901 ccattcacac cctgaccacg ctgcccgtac cgggacggct gttgcctgcg tgtcgtctgg
    15961 cggctgagga acatatacta tgtacgcaaa atcctttctc gctcttgatg gcaacggacg
    16021 tctgacgggg gcccgtaccg cacagactgc gccttacgac cgctacacct gccacctttg
    16081 cggcagtgca ctcagatacc acccgcaata cgacactgaa cgtccctggt ttgaacacac
    16141 tgacgacggg ctgacggagc acggtcatga gtgcccttat gtcagaccgg agcgcaggga
    16201 gatacaactg attaaacgtc tgcagcaatt cgtaccggat gccttacccg tagtacgtaa
    16261 agccagctgg cactgcaggc aatgtcacca cgattattat ggggagcggt actgcacaca
    16321 ctgccagacc gggcgcttca gtgaggaggg gggagcagaa tgaacggtct aaaaacgggg
    16381 ctggggatca cgccgggtga gcacattatc agtgccgact ctgcgctcag cagaaatatc
    16441 cggcagtgtt tctgcctttc ctgtcatggc cggttgattc tgcagacaga tgctcagggg
    16501 gcgtggtttg agcacgattt acatgccctg agcgagcaac agaaggcgga ctgtgtggtg
    16561 ctgaatccgg agaagtccca cccttacatt gaggatatgg cgatgttttt atcgccatta
    16621 ccggtcgtgc tggagtggca ttgtgtgatg tgtgagaggc tttttcatgg gaagaagttc
    16681 tgtgaggcct gtggtacggg gatttattgc agagcggtat gtacaaagtc tgtttacagg
    16741 taccagccag atttgctccg gaacccggag tgaggaggat gcaacataat tgtttaatca
    16801 cgccagacac gcccctgccg gatccggatg tggtccggcg ttttgttctg cggacggtgg
    16861 ttcaatggct gtgtctgctg atgctcctct ttatgcgaca ttcagacagg ggagacatcg
    16921 ataaacatca tctgcgggca aatagttaca cagaatgatc attgctaccg gttatggcaa
    16981 aaattggtga tgaccaggtg aaccatcggt gctttcgttg attcttatgg actgataaag
    17041 ttttgtcagc tgaatcttta tacggaattc ttttcagtat gcgtacacga attgactatc
    17101 ttgcggataa atacagtttt actgaactga atgaacttcc acgccttcac cagcagtggc
    17161 aggatgttct ggaggagtgc cggcagacag aggccgggcc ggaagaacgg ctgcgtattg
    17221 cactgctgaa tgtggattac gtcaccagtt ttgaactgcc ttttcgcttg ttgcttaccc
    17281 gtacaccaca actgattgcc gcgctgcgtg aagaatgggg catcagccag aaaaatgtgg
    17341 tgttcaatga taaacgattt ggctgcgtgt acagcctgaa ggccagcctt tccggtgtgc
    17401 cggatacata ccggtatcat ctgtctcatc gtattcgccg ggtgattggg aatgaaaaca
    17461 catcatcgcc ataccagcag attgcccggg aagtgaaagc gccccgtgaa cggctgaagc
    17521 atgcgctgga ggcggggttg ctggtgactg cactggacgg cctgttctgg ttcggcagtc
    17581 agcgtattgc ggctgatgtc ctgaggttga gaaagagcgg aatgccggtg gtgacaacga
    17641 ccgcggaggt gcatgataat ctgacgggaa caacccgcaa agtaccggcc tactatctct
    17701 gattcgacca tcagggggaa tattgctctg gcagcagcag tacgacattc tttatccccg
    17761 agcctgaaaa catgtgctaa ggaaacggga atggcaaggt ccgattacga tattatcaat
    17821 ctgtctccgg aacatgaact gaatgagtgg ctggcagaga gaggttatgc cggacaggcg
    17881 gataaccgta accgggtggc agaaattgtt acccgcaaat tgcaggacag cttttatatc
    17941 aacgtttcct gggatgcgct gaacacggca tacagtgaac acccggagtg gttttctggg
    18001 cttgtctccg gggatgagaa ttaataagtg gattatgctg ccatcaggca gtgtgattgc
    18061 cggcaggggg ttctttccgg ggttcgggtt tgaattttcc caggaagttg tacatcggcc
    18121 gcatgcaggg atgcggatag ccgtcccgca taaacaccac ccgggtttcc tcaacactgg
    18181 tcacggtgac aagcgcgcca tgatcgtctc tgtatcgctg attgattttt ggtaatggaa
    18241 tatcttctga catagacttc agaacggtaa ccgttcacta aattttcatg tggtatcact
    18301 gaatcaggag acgacgtttc ctgatgtgcg ttgttaattt tctgttttta aaaaatattt
    18361 tctctctctt ctttattttc cgtgtctttt attgctctcc tctttcatgt ccttctcttt
    18421 ttctgatatg cccttaaatg ctgccgaatc atcactcctc gtgaatgtca gtgctggtaa
    18481 tcattcggta taaccatctg tatcaaaagc actaaccgga ataattttct gcatttttgt
    18541 attacccctg gatataagtt ttgataaatg ctgattttgc gatatgtatc agaataaaga
    18601 tctggttatt ggcattaaaa cgatcgatat gtctgtcata ttgagaattt gcagatcttc
    18661 gtcctataat gggtgaaatt aaatcagtgc acaaggggag gccttctgtg gcgatgggct
    18721 ccctgggatg caggtttcac aggagaacgt catgagcagt tcgtttcaga atgagatccc
    18781 gaaggcaagg gtgaatctga aattagacct acatacaggt ggtgggcaga agaaagtgga
    18841 gcttccgctc aaactgctgg ttgccggtga tttcagtaac ggacaggaag agcgcccgct
    18901 gtcagagcgc ataaaaatgg atgttaataa aaataacttt aatgctgttc tggctgatat
    18961 ctcgccgtca gtacgcctga atgtggagaa cacacttgcc ggtgatggta ctgaagagag
    19021 catcagcctg actttccgta atatgaacga tttcacgccg gagctggttg cccgacaaat
    19081 tccccgtctt aaagccatgc tggccatgcg aagtttgctg cgggatctga aggcgaatct
    19141 gctggataac
//
LOCUS       DAZGAN020000090        18958 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGAN020000090 DAZGAN020000000
VERSION     DAZGAN020000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.090"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(75..356)
                     /gene="yehE"
                     /locus_tag="ACHKYO_RS17665"
                     /old_locus_tag="pgaptmp_003533"
     CDS             complement(75..356)
                     /gene="yehE"
                     /locus_tag="ACHKYO_RS17665"
                     /old_locus_tag="pgaptmp_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HGU4430312.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(619..1728)
                     /gene="apbC"
                     /locus_tag="ACHKYO_RS17670"
                     /old_locus_tag="pgaptmp_003534"
     CDS             complement(619..1728)
                     /gene="apbC"
                     /locus_tag="ACHKYO_RS17670"
                     /old_locus_tag="pgaptmp_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HGU4430313.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1860..3893
                     /gene="metG"
                     /locus_tag="ACHKYO_RS17675"
                     /old_locus_tag="pgaptmp_003535"
     CDS             1860..3893
                     /gene="metG"
                     /locus_tag="ACHKYO_RS17675"
                     /old_locus_tag="pgaptmp_003535"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGU4430314.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4034..7827
                     /locus_tag="ACHKYO_RS17680"
                     /old_locus_tag="pgaptmp_003536"
                     /pseudo
     CDS             4034..7827
                     /locus_tag="ACHKYO_RS17680"
                     /old_locus_tag="pgaptmp_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7837..11469
                     /gene="yehI"
                     /locus_tag="ACHKYO_RS17685"
                     /old_locus_tag="pgaptmp_003537"
     CDS             7837..11469
                     /gene="yehI"
                     /locus_tag="ACHKYO_RS17685"
                     /old_locus_tag="pgaptmp_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HGU4430315.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALVCIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLLVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESSWQRCADKLIAALPGIPKIRRPFIALLLPEKPEIANELASL
                     ESSRSSLHSKEWLKVIATDNTAVKKLERCWGLDVFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11530..11850
                     /gene="yehK"
                     /locus_tag="ACHKYO_RS17690"
                     /old_locus_tag="pgaptmp_003538"
     CDS             11530..11850
                     /gene="yehK"
                     /locus_tag="ACHKYO_RS17690"
                     /old_locus_tag="pgaptmp_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HGU4430316.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     LIEY"
     gene            12425..13513
                     /gene="yehL"
                     /locus_tag="ACHKYO_RS17695"
                     /old_locus_tag="pgaptmp_003539"
     CDS             12425..13513
                     /gene="yehL"
                     /locus_tag="ACHKYO_RS17695"
                     /old_locus_tag="pgaptmp_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HGU4430317.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            13524..15802
                     /gene="yehM"
                     /locus_tag="ACHKYO_RS17700"
                     /old_locus_tag="pgaptmp_003540"
                     /pseudo
     CDS             13524..15802
                     /gene="yehM"
                     /locus_tag="ACHKYO_RS17700"
                     /old_locus_tag="pgaptmp_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            15795..16931
                     /gene="yehP"
                     /locus_tag="ACHKYO_RS17705"
                     /old_locus_tag="pgaptmp_003541"
     CDS             15795..16931
                     /gene="yehP"
                     /locus_tag="ACHKYO_RS17705"
                     /old_locus_tag="pgaptmp_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU4430318.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            16928..18928
                     /gene="yehQ"
                     /locus_tag="ACHKYO_RS17710"
                     /old_locus_tag="pgaptmp_003542"
     CDS             16928..18928
                     /gene="yehQ"
                     /locus_tag="ACHKYO_RS17710"
                     /old_locus_tag="pgaptmp_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HGU4430319.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         4709 a         4930 c         4854 g         4465 t
ORIGIN      
        1 atcaataaaa aagccgaaat catttatata aatgatttcg gctttctaaa tgcaattaaa
       61 atcaggttgc caaattaatc gtagcgattt aacacgattc gacgtttgct atcgctacct
      121 gcaacaacca cttctgtcgt tacacctttg acgggcgaag aaaacagatg acgggtgtca
      181 acattatacg ttaactgacc acaatgctgt tgaggttgac cattaaccat agccatactg
      241 catgttgggg gactgatcct gccattaatc gctaatgtgg cagtctctga agagaatgcg
      301 ggagaagcca atccataggc aagaaaaata attcctgaca gccaatactt attcattgaa
      361 cgttatccct gtagtaaagg ttatgcctgg atagaatgag tgcataacaa actatagctg
      421 tacatccact acacagccac gaaggatgat aatgaagcat tgcctgtatg atcaatcgac
      481 tttgtagagt ttcggacgaa ggtccgcaga atattcgcag tattaaataa gtgttcatta
      541 atgcggcaaa aactaataca ccgcatcaat gtaacatctc tgcggtgtat gaagaataac
      601 cagaatggtt aataggcgtt agaccgcgcg gaaggaaatc tcgcctggaa tgacttcacc
      661 ctgccagtag agctgagctg caacgcggtc agccagctgg cggtagatag cggtaaattc
      721 gctctccgga cggctgataa ccgttggcgt gcctttatcg agatcctcac gtaaggagat
      781 gtgtaacggc atctgcccca gcagctgtgt gtggtatttt tcagccagtt tctcagcacc
      841 accggtaccg aaaatcggtt catggtgacc acagttactg caaatatgca cactcatgtt
      901 ctcgacaata ccaagcaccg gaacctcgac tttctcgaac atcacaatgc cttttttcgc
      961 atcgatcaac gcgatgtcct gcggggtggt gaccacgact gcaccagtta ctggaatgtt
     1021 ttgcgccagc gtcaattgaa tgtcaccggt gcctggcggc atatcaagca ccagatagtc
     1081 gagatccggc cacaaggttt cctgcaacat ctgcattaac gccttgctcg ccatcggtcc
     1141 acgccacacc atcgcattgt cgtcagtgac cagataacca attgaattgg ttgccaggcc
     1201 atgagacata atcggtgcca tgtgggtacc gtccggcgac gttggacgct gattttctgc
     1261 gcccagcatg gtgggaattg atgggccgta gatatcggcg tccagaatgc cgactttcgc
     1321 cccttccgcc gccagtgcca gcgccaggtt taccgccgtg gaggatttac ccacgccgcc
     1381 tttaccggag ctgaccgcaa tgatgttctt cacaccatta atacccggct gattcttcac
     1441 gcgttttaag gtggcgatat tgtgcgacag tttccagtcg atagctttcg ccccggtgat
     1501 acgcagcagt tcggcgctac attgctcttt taactcttca aacgcgctat gccagacgaa
     1561 tggcataacc agttccacat gcaacgtgtc gtccatccag gcgacatggt gcaacgcttt
     1621 cagcgtggtg aggttatgct tcagggttgg gtgctgaaaa ttggccagtg tcccggcgac
     1681 cattgcgcgc aaggcttctg gtgatttggc ctgggattgt tcgttcatcc cgactccttt
     1741 tttgtataga taaaccatca gctgatagtt tacctgaaga atatagagaa gtacttactt
     1801 aacattttcc catttggtac tatctaaccc cttttcacta ttaagaagta atgcctacta
     1861 tgactcaagt cgcgaagaaa attctggtga cgtgcgcact gccgtacgct aacggctcaa
     1921 tccacctcgg ccatatgctg gagcacatcc aggctgatgt ctgggtccgt taccagcgaa
     1981 tgcgcggcca cgaggtcaac ttcatctgcg ccgacgatgc ccacggtaca ccgatcatgc
     2041 tgaaagctca gcagcttggt atcaccccgg agcagatgat tggcgaaatg agtcaggagc
     2101 atcagactga tttcgcaggc tttaacatca gctatgacaa ctatcactcg acgcacagcg
     2161 aagagaaccg ccagttgtca gaacttatct actctcgcct gaaagagaac ggttttatta
     2221 aaaaccgcac catctctcag ctgtacgatc cggaaaaagg catgttcctg ccggaccgtt
     2281 ttgtgaaagg cacctgcccg aaatgtaaat ccccggatca atacggcgat aactgcgaag
     2341 tctgcggcgc gacctacagc ccgactgaat tgatcgagcc gaaatcggtg gtttctggcg
     2401 ctacgccggt aatgcgtgat tctgaacact tcttctttga tctgccctct ttcagcgaaa
     2461 tgttgcaggc atggacccgc agcggtgcgt tgcaggagca ggtggcaaat aaaatgcagg
     2521 agtggtttga atccggcctg caacagtggg atatctcccg cgacgcgcca tactttggtt
     2581 ttgaaattcc gaacgcgccg ggcaaatatt tctacgtctg gctggacgca ccgattggct
     2641 acatgggttc tttcaagaat ctgtgcgaca agcgcggcga cagcgtaagc ttcgatgaat
     2701 actggaagaa agactccacc gccgagctgt accacttcat cggtaaagat attgtttact
     2761 tccacagcct gttctggcct gccatgctgg aaggcagcaa cttccgcaag ccgaccaacc
     2821 tgtttgttca tggctatgtg acggtgaacg gcgcaaagat gtccaagtct cgcggcacct
     2881 ttattaaagc cagcacctgg ctgaatcatt ttgacgctga cagcctgcgt tactactaca
     2941 ctgcgaaact ctcttcgcgc attgatgata tcgatctcaa cctggaggat ttcgttcagc
     3001 gtgtgaatgc cgatatcgtg aacaaagtgg ttaacctggc ctctcgtaat gcgggcttta
     3061 tcaacaagcg ttttgacggc gtgctggcaa gcgaattggc tgacccgcag ttgtacaaaa
     3121 ccttcactga tgccgctgaa gtgattggtg aagcgtggga aagccgcgaa tttggtaaag
     3181 cagtgcgcga aatcatggcg ctggctgatc tggctaaccg ctatgtcgac gagcaggctc
     3241 cgtgggtggt ggcaaaacag gaaggccgcg atgccgacct gcaggcaatt tgctcaatgg
     3301 gcataaacct gttccgcgtg ctgatgactt acctgaagcc ggtactgccg aaactgaccg
     3361 agcgcgcaga agcattcctc aatacagaac tgacctggga tggtatccag caaccgctgc
     3421 tgggccacaa agtgaatccg ttcaaagcgc tgtataaccg catcgatatg aagcaggttg
     3481 aagcactggt ggaagcgtct aaagaagaag tgaaagcagc tgccgcgcca gttactggcc
     3541 cactggcaga tgatccgatt caggaaacca tcacctttga cgacttcgca aaagttgacc
     3601 tgcgcgtggc gctgatcgaa aacgcagagt ttgttgaagg ttctgacaaa ctgctgcgcc
     3661 tgacactgga tctcggcggt gaaaaacgca atgtcttctc cggtattcgt tctgcctacc
     3721 cggatccgca ggcactgatc gggcgtcaca ccattatggt ggccaacctg gcaccgcgta
     3781 aaatgcgctt tggtatctcc gaaggcatgg taatggctgc tggtcctggc gggaaagata
     3841 tcttcctgtt aagcccggat gctggtgcta aaccaggcca tcaggtgaaa taatccccct
     3901 ttaaggcgct gcatcgacag cgccttttct ttataaattc ctaaagttgt tttcttgcga
     3961 ttttgtctct ctctaacccg cataaatact ggtagcatct gcattcaact ggataaaatt
     4021 acagggatgc agaatgagac actttatcta tcaggacgaa aaatcacata aattctgggc
     4081 agttgagcaa cagggaaacg agttgcatat cagttgggga aaagttggca ccaaagggca
     4141 aagccagata aaaagttttt cagatgctgc ggcagtggca aaagcggagc ttaagctgat
     4201 tgcggagaag gtgaagaagg ggtatgtgga gcaagcgaag gataattctt tgcaaccttc
     4261 ccaaacggta acgggctctc tcaaggtagc ggacttatcc accattattc aggaacaacc
     4321 ctctttcgta gcagaaaccc gtgcgcctga caaaaataca gatgctgttt taccgtggct
     4381 ggcgaaagat attgctgtcg tttttccgcc cgaagttgta cacaccacgt taagtcatcg
     4441 ccgctttccc ggagttcctg ttcagcaagc agacaaattg acccaattac gtcgcttagc
     4501 ctgtagtgtg tcgcaacggg ataataaaac agccacattt gacttcagcg cctgttcttt
     4561 agaatggcaa aacaccgtcg cccaggcgat cagtcagatc gacggcctga aaacaacaca
     4621 gctaccatca ccagtaatgg ctgtactcac ggcacttgaa atgaaatgca caagatttaa
     4681 agtgcgtgag gatgttatgg atcagatcgt ccaggaaggc ggtctggaat atgctactga
     4741 tgtaataata caccttcaac agattgatat taaatgggat tatgcgaata atgtcattat
     4801 tattctgccg tctggcattg cacctgacta cttggagcaa tattccagat ttgaattacg
     4861 cctacgtaaa catttatcac tggcggaaga gtctctctgg caaaaatgtg cacaaaaact
     4921 tattgccgca attccacata ttccagaatg gcggcaacca ttaattgctt tgttattacc
     4981 cgaaaaacca gaaattgcac atgaaattgc ccagcgtcta ctggggcaaa aaaaattacc
     5041 ctcgcttgag tggttaaaaa tagtggcgac tgatgagcac attcttgcct cattagaaaa
     5101 atatcacgag ccatatgcca tttttgatga ttactattgt ggtgcgatat ggtcagccac
     5161 cgtattacag gagcaaggtg ttgcagccct gccccgattt gctccctatg ccgcaagtga
     5221 ctactgcgcc gatgtgttgc gtcatatcaa tcatccgttc gcattaacac tgcttatacg
     5281 tgtagccggg catactaaac gctgtcacga tcggatgacg aaagcctgcg ctgcgttccc
     5341 acatgcagca atggcggcac tgacggaact tcttgggcaa aaagaagaga acagttggcg
     5401 cattatgcta atgacaatgc ttatctcaca accagcactg gcagaacagg tcattccctg
     5461 gctctcgaca cccgcagttg ccgtactgaa atcatgccag caacaactga cacagccctc
     5521 aaaccatgcc agcgccgatc tactgccagc cgtagtagtc tcccctccct ggctttcgaa
     5581 aaagaaaaaa tcgccgattc cggtgctgga tttagcgcca ttaggcattg agccaatctg
     5641 ttatctgaca gaagaaatca gtaatcaact tttggcgaaa tatatctggt attcaaaaca
     5701 catcacggtt agccatgaag aaagtactac caacctgttg gcaaggatgg gttttcaacg
     5761 acggatcgct ggtacatata ttaaagctcc cgaagcggta gttgaggcat ggctaaatga
     5821 agattattca accttactaa gtgaatttaa ggtgtttcat tcacctaccg ggcattattg
     5881 gcagttgggg attttgacaa cattgccgct ggagaaagca gtaaaagcat ggaatgccct
     5941 taccctatct ccacataccg ataccgaata cgccatgtta cattttggac tcaaagggtt
     6001 acctgggtta gtaaactcac ttgcacgcta tccacaagaa gccttgccca tcacgaatta
     6061 cttcgcagcg agtgagctgg ctcctgccgt cgcccgtgcc ttcaacaaac tgaaaacgct
     6121 acgcgaaaac gcccgtagct ggctgttgaa atacccggaa catgccctta ccggcctgct
     6181 gcctgcggcg ctcggcaaag ccggtgaagc acaggataac gcccgcgctg ccttgcgtat
     6241 gcttaccgaa aacggtcatc agccattact gcaagaaatc gcccgacgtt ataaccagcc
     6301 ggaagtaacc gatgcggtga acgctctgct tgcgctcgat cccttagata atcacccgac
     6361 aaaatcccca ctcttccggc cttttatcag ccatcgctct ggacgcgccc ggtattaaaa
     6421 gcaaatgccc aatcactgcc agatagcgcc ctcctccacc tcggtgaaat gctccgcttc
     6481 cctcaggaag aggctctgta tccgggatta ttgcaggtga aagacgtctg ttccgccgac
     6541 tcactggcgg gatttgcctg ggatctgttt accgcctggc agaccgctgg cgcgccgtcg
     6601 aaagagagtt gggcgttcac tgcgttaggc gttctcggta acgatgacac cgcccgcaaa
     6661 ctgacgccat taatacgcgc ctggcctggt gaatcccagc ataaacgcgc caccgttggg
     6721 ttggatattc tcgctgctat cggtagtgat atcgccctta tgcagcttaa cggcatcgcc
     6781 cagaaactga aattcaaagc attacaggag cgggcaaaag aaaaaattgc cgacattgcc
     6841 gagagccgcg aactcacggt ggcggagctt gaagatcggt tagcaccgga tctcggtctg
     6901 gatgataacg gttcgctgct gctggatttt ggcccacggc agttcaccgt cagctttgat
     6961 gaaaccttaa aaccgtttgt gcgtgatgtt tccggcagcc gcctgaaaga cctgcccaaa
     7021 ccgaacaaaa gcgatgatga aacgcgggcg aacgatgcgg ttaaccgcta caaattgctg
     7081 aaaaaagatg cgcgtaccat cgccgcccag caggtagcaa ggctggaatc cgccatgtgc
     7141 ctgcgccgcc gctggtcgct ggaaaacttc cagctcttcc tggttgagca tccgctggtt
     7201 cgtcacttaa cccgccgtct gatttggggc gtttatagcg ccgaaaacca gctactggct
     7261 tgctttcgcg tagcagaaga taacagctac agcaccgctg acgatgatct tttcaccctg
     7321 ccggaaggcg atatctctat cggcactcct cacgttctgg aaatatcacc aacggatgct
     7381 gccgcctttg gtcagctttt tgccgactac gaactgctac caccgttccg ccagctcgac
     7441 cgtaacagct acgccctgac agaagccgag cgcaatgcca gtgaactgac ccgctgggca
     7501 ggcagaaaat gcccgagcgg tcgggtcatg ggactggcga ataaaggttg ggtgcgtggt
     7561 gaaccgcagg atggtggctg gatcggctgg atgatcaaac ctctgggccg ctggtcgtta
     7621 atcatggaaa tcgatgaagg ctttgcagtt ggtatgtcgc cagccgaact tagcgccgag
     7681 caactcttaa gcaagctgtg gctatgggaa ggcaaagcag aaagctatgg ctgggggagt
     7741 aattcaacac aggaagcgca gttctccgta ctcgatgcca tcaccgccag cgagctgatt
     7801 aacgatattg aagccctgtt tgaataagga aagagcatgg acaaggaatt accgtggctg
     7861 gcggataacg cccaactgga actgaaatat aaaaaaggca aaacaccact cagccatcgc
     7921 aattggccgg gcgaaccagt gcctgttatc actgaaagca tcattcagac actgggtgat
     7981 gaattgctac aaaaagctga gaagaaaaaa aacattgtct ggcgttatga gaatttttca
     8041 ctggagtggc agtccgccat tacgcaggcc atcaacttga tcggcgaaca caaaccctca
     8101 atcccggccc ggacaatggc ggcgctagtc tgtatcgcgc aaaatgacag ccaacagttg
     8161 ctcgacgaaa tcgtccaaca agagggactg gaatatgcga ctgaggtggt gattgcacgc
     8221 cagtttatta cacgatgtta tgagagtgat cctctgctag tgacattgca gtatcaggac
     8281 gaggattatg gctatggtta tcgctcagaa acctataacg aattcgatct ccgactgcgt
     8341 aagcatctct ctctggcaga ggaaagcagc tggcagcgtt gcgccgacaa actcattgcc
     8401 gcactgccag gaatacccaa aattcgccgt ccttttattg cactactcct cccggaaaaa
     8461 cctgaaatag ccaatgaact cgccagtctt gaaagctccc ggtcaagtct ccattcaaag
     8521 gagtggttaa aggtcattgc tactgataat acggcggtaa aaaaactcga acgatgctgg
     8581 ggcctggacg tatttagcga tcgagaagcc agctacatgt cgcatgaaaa ccacttcggc
     8641 tacgcggcct gcgccgccct tttgcgcgaa caaggactgg cagccgttcc gcgcctcgcg
     8701 atatatgccc ataaagaaga ttgcggcagt ctgctggtac aaattaacca tccgcaagtc
     8761 atccgcacct tgctgttagt tgctgataaa aacaaaccca gcctgcaacg tgtggctaaa
     8821 tacagtaaaa acttccccca tgcgacactc gccgcactgg cagaactgct ggcgttaaaa
     8881 gaaccaccag cccgcccagg ttatccaatc atcgaagaca aaaagctgcc tgcacagcaa
     8941 aaagcacgcg atgaatactg gcgtacgctg ttacaaacgc tgatagcatc gcagccacaa
     9001 ctagcagaaa gggtgatgcc gtggttaagt actcaagcca gggcagtgct gaatagttat
     9061 ttatcggcac cgccaaaacc ggttattgat agcaccgata acagcagtct gcctgaaatg
     9121 ctcgtttcac caccgtggcg tagtaagaaa aaaatgacag ctccacgtct tgatttggca
     9181 ccgctcgaat taactccgca aatttactgg caaccaggcg aacaagagag gcttgccgcc
     9241 actgagtctg cccgttattt cagcacggaa tctcttgcgg aacgcatgga acaaaaaagt
     9301 ggacgagttg tattacagga actgggtttt ggggatgatg tatggctgtt tctgaattat
     9361 atactccccg gaaaactgga tgctgcacgc aattcactca ttgttcagtg gcattactac
     9421 caggggcggg ttgaagagat cctgaatggc tggaactccc cgcaagcaca attagcagaa
     9481 caggcgctcc gcagcggtca catagaagcg ttaattaaca tatgggaaaa tgacaacttc
     9541 tcacgttatc gtccggaaaa gagtgtctgg aacctgtatt tattggcaca gttgccgcgt
     9601 gagatggctt tgaccttctg gctgcgtatc attgagaaaa agcatctgtt cgcgggtgag
     9661 gactattttc tcagtatcct cggattggat gcgctaccag gtctgctgtt ggctttttca
     9721 catcgtccaa aagaaacatt tccgttaatt ttaaattttg gcgcaacaga actggcgctg
     9781 cctgttgccc gcgtctggcg acgtttcgcg gcgcagcgtg atctggctcg ccagtggatt
     9841 ttacaatggc cggaacatac ggctagtgca cttatccctc ttgtctttac caaacccagc
     9901 gataatagcg aagccgcatt acttgccctg cgtttactgt acgaacaggg acatggcgaa
     9961 ttgctacaaa ccgtggcaaa ccgctggcag cgtacagatg tatggtctgc cctggagcag
    10021 ttgcttaaac agggtccaat ggacatttac ccggcacgca ttccaaaagc ccctgatttc
    10081 tggcatccgg caatgtggtc caggcctcga cttatcacta ataatcagcc tgttaccggt
    10141 gacgctctgg aaattatcgg cgaaatgctg cgctttaccc aggggggacg tttttatagc
    10201 gggctggaac aactgaaaac gttctgccag ccacaaacgc tggcagcttt tgcctgggat
    10261 ctcttcactg cgtggcaaca agctggtgcc cccgcaaaag acaactgggc atttctggcg
    10321 ttaagtctct ttggtgacga aagcacggca cgggatctga cgacacagat cctcgcctgg
    10381 ccacaagaag gcaaatctgc ccgtgctgtc agcggcctga acatccttac cctgatgaat
    10441 aatgatatgg cgctgataca gctgcatcat atatcgcaac gggctaaatc ccgcccctta
    10501 cgtgataacg cggcggaatt tcttcaggtg gtcgcagaaa atcgcgggct aagccaggaa
    10561 gagctagcgg acagattagt cccaaccctg ggccttgatg atccgcaggc gttgagtttt
    10621 gattttggtc cccggcagtt taccgttcgc ttcgatgaaa acctcaaccc ggttatcttt
    10681 gatcagcaaa acgttcgcca gaaaagcgtt ccccggttgc gcgccgatga cgatcaactg
    10741 aaagcgcccg aggcactggc ccgactaaaa gggctaaaaa aagatgctac tcaggtgagc
    10801 aaaaacctgc tcccgcgtct tgaagctgcc ctacgtacca cccgacgctg gtcgctggca
    10861 gattttcata ctctgtttgt taatcatccc tttacccgcc tggttaccca gcgattaata
    10921 tggggcgtgt atccggcaaa tgaaccgcgt cgtttactca acgcctttcg tgtggccgca
    10981 gagggggagt tctgcaatgc gcaggatgag ccaattgacc tgcctgcgga tgctctgatt
    11041 ggcattgccc acccgttaga aatgacagca gaaatgcgca gtgaatttgc ccagcttttt
    11101 gccgattacg aaattatacc gccacttcgc cagttgacac ggcgcacggt gctgctcacg
    11161 cctgacgagt cagccagtaa tagtttgaat cgctgggaag gtaaatctgc taccgttggg
    11221 caacttatgg gaatgcgata caaaggctgg gagtcaggct atgaggacgc atttgtctat
    11281 gacctgggtg agtaccggct ggtccttaag ttttcacccg gttttaacca ctacaatgtt
    11341 gatagcaaag cgctaatgag cttccgttct cttcgagtgt acagtgacaa taaatccgtc
    11401 acttttgccg aacttgatgt gtttgatttg agtgaggcgt taagcgcacc tgacgtcatt
    11461 ttccattaaa acacagcggg cagtgatgca actgcccgtt atcaacacga cttaccagcg
    11521 gggaaagcga tgatcgtgca aaaagagctg gttgctattt acgattatga ggtccctgta
    11581 cctgaagatc cgttttcctt cagacttgag atccataaat gctctgaatt atttacaggt
    11641 tccgtctatc gactggagcg attccggcta cgtccaacat ttcatcaacg tgatcgagaa
    11701 gatgctgacc cgctaataaa tgatgcgttg atttatataa gagatgagtg tattgatgag
    11761 cggaaattac gaggtgaatc acctgaaact gtaatagcaa tttttaatcg tgaactacag
    11821 aatatattca accaactaat agaatattag tttattaaag ggggaagcgg ttttccccct
    11881 tagatgcgtt tcaatttgcc cgttacttca acaaatcaat caaccgtaac agagccacta
    11941 atagctccag tacagcaatg agcacggtta acttttgtgt cagcgtgtcc atccgctctc
    12001 cttattaagg agcgcaacac cagcacctta cccttacact gcctgtcgat actggtttac
    12061 gtatgttgga ccacgatgtt gcactccgga tcagggcacc acctgcagca ccacccgtcc
    12121 ggtagccagg acttgaaata gattcgatcc ggcgcaacgt cccaacggtc tggattataa
    12181 atctcattat cttaattgca acggggtcca gccgtggtta taattcgttc gttggaacac
    12241 gatgttgcac aggctgtggt gttaggcctg aaagtagtaa ataaaagaac ctgcctcacc
    12301 agcaggtttt tttatttact gtgatctgct ttccagatat ttttcgctca aacaactaat
    12361 gcgccaaaca tttattgcgc gtaaaatatc gtttatttca ttaatacatt tcagggatga
    12421 atatatgtca ccgcagaata atcatctgca gcgtccgcct gctgctgtgt tatacgccga
    12481 tgaactggca aaattaaaac aaaatgataa cgcaccttgc ccgcccggtt ggcagttaag
    12541 tttgcctgcg gcccgtgctt ttatccttgg cgatagcgcg caaaatatta gccgtaaagt
    12601 ggtgattagt ccctccgctg tcgaacgcat gttagtcact cttgccaccg ggcgcggttt
    12661 gatgttggtg ggggaacctg gcaccgcaaa atctctcctg tctgaattac tggcaaccgc
    12721 tattagcggc gatgccgggt taaccatcca gggcggcgca tctactaccg aagatcaaat
    12781 caagtacggc tggaactacg ccctgcttat caaccatggt ccgtcaacgg aagcactggt
    12841 ccctgccccg ctttaccagg gaatgcgcga cggcaaaatc gtccgttttg aagagataac
    12901 gcgtacaccg ctggaagtac aagactgtct gttaggtatg ctttctgaca gggtgatgac
    12961 ggtgccggaa ctcaccggtg aagccagtca gctctatgcg cgggaaggct ttaatattat
    13021 tgccactgcc aatacccgcg atcgcggcgt caatgagatg agtgccgcgc taaagcgtcg
    13081 cttcgatttt gaaaccgtct ttccgattat ggatttcgcc caggagctgg aactggtcgc
    13141 cagtgcttcg gcgcgtttgc tggcccatag cggtattccg cataaagtgc ctgacgcagt
    13201 actggagtta ctggtacgca ccttccgcga tctgcgtgcc aacggcgaaa agaaaacgtc
    13261 aatggataca ctgacagcga ttatgtccac cgctgaagcc gtgaacgtgg cccacgccgt
    13321 aggcgtccgc gcctggtttt tagcgaatcg cgcgggggag cctgccgacc tggtggactg
    13381 tattgccgga accatcgtca aagataacga agaagatcgc gctcgtctgc gccgttactt
    13441 cgaacagcgt gtcgccacac ataaagaagc tcactggcag gcttattatc aagcccgcca
    13501 ccgcctgccg tgaggaaaga ggcatgagcg agccgttaat tgtcggcatc cggcatcata
    13561 gtccggcctg cgcccggctg gtgaaatcgt taatcgaaag ccagcggcca cgatacgtgt
    13621 tgattgaagg cccggctgat tttaatgacc gggtagacga actttttttt cccaccagct
    13681 tccggtagct atttacagtt attgccagta tcaggacggt gcagcccccg ggcgtggtgc
    13741 ctggacgcca tttgctgaat tttcgccgga gtggcaggcg ctacaagccg cacgtcgtat
    13801 tcaggcacaa acttacttca tcgatttgcc ttgctgggcg cagagtgaag aagaggacga
    13861 ttcgcctgat acgcaagatg aaagccaggc cttactgctg cgtgccaccc gcatggataa
    13921 cagcgatacc ctgtgggatc acttgttcga agatgaaagc cagcaaactg cattaccctc
    13981 tgcgctggcg cactattttg cccaactgcg gggcgatttc cccggcgatg cactcaatcg
    14041 tcagcgcgaa gcctttatgg ctcgctggat tgcatgggcg gtgcagcaaa ataatggcga
    14101 cgtgttagtc gtctgcggtg gctggcacgc tccggcactg gcaaaaatgt ggcgcgaatg
    14161 cccgcaggac attaacacgc cggaattgcc ctcgctggca gatgccatta caggttgtta
    14221 tctcacgccc tacagtgaaa agcgccttga tgtgctggca ggataccttt ccggtatgcc
    14281 tgccccggtc tggcaaaact ggtgctggca gtggggctta cagcaggccg gtgaacaact
    14341 gctaaaaacg gttctcaccc gtttgcgcca gcacaacttg cctgcttcga ccgcggatat
    14401 ggctgccgca catctgcatg caatggcact ggcacagttg cgcggtcata cactaccgtt
    14461 acgcactgac tggctggatg ccatagcagg ctcgttgatt aaagaagccc tgaatgcacc
    14521 gttgccgtgg agctatcgcg gcgttattca tcccgatacc gatccgattc tgctaacgtt
    14581 gatagacaca ttagcgggtg acggattcgg taaacttgcc ccttccacgc cacaaccgcc
    14641 tctgccaaaa gatgtcacct gcgaactgga acgtaccgca atctctcttc cggcggagct
    14701 taccttaaat cgctttaccc ccgatgggct ggcgcaaagt caggtgttac atcggctggc
    14761 aatactggag atccctggga ttgtacgcca gcagtgaagt acactgacac ttgcaggcaa
    14821 cggtgaagaa cactggaaat taacccgccc gcttagccag catgcggcat tgattgaggc
    14881 cgcatgcttt ggtgcaacac ttcaggaagc cgcacgccat aaattagaag ccgatatgct
    14941 ggacgcgggt ggaatcggca gtatcaccac atgtcttagc caggcggcgt tagcgggtct
    15001 ggcgtccttc agtcaacaat tactggagca actcacatta ttaatcgccc aggaaaatca
    15061 atttgccgaa atgggccagg cgctggaagt gctatatgcc ttatggcggc tggatgaaat
    15121 tagcggtatg caaggcgcgc agatattaca aacgacgtta tgcgcggcta tcgatcgcac
    15181 gctgtggctg tgtgaatcta acggcagacc ggatgaaaag gagtttcacg ctcacctgca
    15241 tagctggcaa gcgctttgcc atattctgcg cgatctacat agcggcgtta atttatccgg
    15301 cgtttcgctt tctgcggcgg tagccttact ggagcgacgc agtcaggcga ttcatgcccc
    15361 ggcgctggat cgcggcgcgg ctcttggcgc actaatgcgt ctggaacatc ccaacgccag
    15421 tgccaaagcg gcgctgacga tgctggcgca gttatccccg gcacaatccg gcgaggcgct
    15481 gcacggtttg ctggcattag cccgtcatca actggcctgt caacctgcat ttatcgccgg
    15541 tttcagcagc catttgaatc aactgagtga tgccaatttt atcaatgccc tgcccgattt
    15601 acgcgcggcg atggcctggc taccgccacg agaacgcgga acgctggcgc atcaggtgct
    15661 tgagcattat caactggcgc aacttcccgt ttcggcactg caaatgccgt tgcattgtcc
    15721 accgcaagcc attgcacatc atcaacaact cgaacagcag gcactggcat cgctgcaaca
    15781 ctggggagtt ttccatgtct gaactgaacg atcttctgac cacccgtgag ctacaacgct
    15841 ggcgattaat tcttggcgaa gcggcagaaa cgacgctttg tgggctggat gacaacgccc
    15901 ggcagataga tcacgcgctg gagtggctgt atgggcgcga tcctgaacgg ctccagcgtg
    15961 gtgaacgctc tggtggatta ggtggctcaa atctcaccac tcctgagtgg atcaacagta
    16021 tccacacgct gtttccgcaa caggtgattg agcggctgga aagcgatgcc gtactgcgct
    16081 acggcattga agatgtggtg acaaatctcg acgtgctgga acgtatgcag ccttcggaaa
    16141 gcctgctacg cgccgttttg cacaccaaac atctgatgaa tcccgaagta ctggctgccg
    16201 cccgccagat agtgcgccag gttgttgaag aaattatggc gcgactggca aaggaagttc
    16261 gtcaggcttt ttctggtgtc cgcgatcgcc gtcgccgctc attaattcca ctggcgcgaa
    16321 actttgattt caaaagtact ctgcgcgcca acctgcaaca ctggcacccg caacacggca
    16381 agttgtatat cgaatccccc cgctttaaca gccgcattaa acgccaaagc gaacaatggc
    16441 aactggtctt actggttgat caaagcggat cgatggtgga ctcggtgatc cactctgcgg
    16501 tgatggcggc ctgtttgtgg cagttacccg gcattcgtac ccatctggtg gcgtttgaca
    16561 caagcgtggt tgatctcacg gcagacgttg ccgatccggt agagttatta atgaaagtac
    16621 agttgggcgg cgggaccaat atcgccagtg ccgtggagta tggtcggcaa cttattgaac
    16681 aaccagcaaa aagcgtcatt atcctcgtga gcgattttta cgaagggggt tcatcatcat
    16741 tgctgacgca tcaggtgaaa aagtgtgtcc agagcggcat caaagtgctg ggactggcag
    16801 cgctcgatag caccgcaacg ccttgctatg accgcgatac ggcccaggcg ctggttaatg
    16861 tcggcgcaca aatagccgcc atgacgccgg gcgagctggc atcatggctt gcggagaatc
    16921 ttcagtcatg aattcactac gtccggaatt attagaactg acaccgcagg cactgacggc
    16981 gttaagcaat gccggttttg ttaagcgcag tcttaaggaa ctggaaaatg gcaacgtccc
    17041 ggaaatcagc catgagaacg gcgctttaat cgccaccttc agtgacggtg tccgtaccca
    17101 gctggcgaac agccaggcac tgaaagaggc tcagtgcagc tgcggggcca gcggcatgtg
    17161 ccgtcatcgc gtgatgctgg tgttaagtta tcaacgactt tgtgccaccg tccagcccac
    17221 ggaaaaagaa gaagagtggg atccggcaat ctggctggaa gaactggcta ccctgcccga
    17281 tgccacccgc aaacgcgcgc aggcgctggt cgctaaaggc atcaccattg agttgttctg
    17341 tgcgccgggc gaaattccct ctgcccgctt accgatgagc gatgtgcgtt tttattcccg
    17401 tagcagtatt cgtttcgccc gttgtgattg tattgaaggc acactttgcg aacatgtcgt
    17461 actggctgtg caggccttcg tcgaggccaa agcgcagcaa gcagaattta accatttaat
    17521 ctggcagatg cgcagcgaac acgtcacatc atctgacgat ccgtttgcca gcgaagaagg
    17581 ccaaacgtgt cgtcaatatg ttcagcaatt aagccaggca ttatggcttg gcggcatcag
    17641 ccagccgctt attcactacg aggcagcatt caaccgcgca ttgcaggcgg cagaggcctg
    17701 caactggcgc tgggtgagtg aatcactacg acagctacgg gcaagcgttg atgccttcca
    17761 cactcgcgcc agccactatc atgccggaga atgcttacgt cagcttgcgg cattaaacag
    17821 tcgattaaat tgcgcacaag agatggcccg gcgcgacagt gttggtgaag ttcctcctgt
    17881 gccgtggcgc acggtcgttg gctctggcat tgccggagaa gcaaagcttg atcatctgcg
    17941 gctggtgtct ttaggtatgc gttgctggca ggatattgag cattatggtt tacgcatctg
    18001 gtttaccgat cccgacaccg gcagtatttt gcacctttcg cgcagttggc cgcgaagtga
    18061 acaggaaaac tcaccggcag ctacgcgtcg gctgtttagt tttcaggctg gcgcactggc
    18121 gggtgggcaa attgtttcac aagcagcaaa acgcagtgcc gatggcgagc tgctgttagc
    18181 tacccgcaac cgcttaagca gcgttgtgcc gctgtcgcct gatgcctggc aaatgttgag
    18241 cgcgccgtta cgccagccgg gcattgtggc tttgcgggaa tatttacgcc agcgtccccc
    18301 cgcctgcata cggcctctta atcaggtcga taacttattt attctgccgg tcgctgagtg
    18361 tatttcgctc ggttgggata gcagccgcca gacgctggat gcgcaggtaa tcagcggcga
    18421 aggtgaagat aatctcctga cgttatccct tccggcatct gcctgttctc cttttgccgt
    18481 tgaacgcatg gcggcgcttt tgcaacaaac agacgacccc gtgagtctgg tttctggctt
    18541 tgtcagtttt gttgatgggc aattgacact ggaaccacgg gtgatgatga caaaaacccg
    18601 cgcctgggcg ctggacgcgg aaactgcgcc tgtggcaccg ctaccttctg ccagcgtttt
    18661 gcctgtgccg tctaccgctc atcagttgct gatgcgctgc caggcgttac ttattcaact
    18721 gctccataac ggctggcgct atcaggaaca gagcgctatt agtcaggcag agttgctggc
    18781 gaatgacctc tccgcggtcg gtttttatcg gctggcacat gtgttgggac aatttcgtaa
    18841 tacagaaagc gaggcacggg tagaagcaat gaataacggt gttttacttt gcgaacaatt
    18901 attccccatg cttcagcaac aaggatgaaa tagtgctttt tactaagagt tctactcc
//
LOCUS       DAZGAN020000091        18880 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGAN020000091 DAZGAN020000000
VERSION     DAZGAN020000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.091"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1072)
                     /gene="nemA"
                     /locus_tag="ACHKYO_RS17715"
                     /old_locus_tag="pgaptmp_003543"
     CDS             complement(<1..1072)
                     /gene="nemA"
                     /locus_tag="ACHKYO_RS17715"
                     /old_locus_tag="pgaptmp_003543"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HGU4430320.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAE"
     gene            complement(1109..1708)
                     /gene="nemR"
                     /locus_tag="ACHKYO_RS17720"
                     /old_locus_tag="pgaptmp_003544"
     CDS             complement(1109..1708)
                     /gene="nemR"
                     /locus_tag="ACHKYO_RS17720"
                     /old_locus_tag="pgaptmp_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HGU4430321.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            1811..2050
                     /locus_tag="ACHKYO_RS17725"
                     /old_locus_tag="pgaptmp_003545"
     CDS             1811..2050
                     /locus_tag="ACHKYO_RS17725"
                     /old_locus_tag="pgaptmp_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HGU4430322.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPKQPSLF"
     gene            2099..2995
                     /locus_tag="ACHKYO_RS17730"
                     /old_locus_tag="pgaptmp_003546"
     CDS             2099..2995
                     /locus_tag="ACHKYO_RS17730"
                     /old_locus_tag="pgaptmp_003546"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HGU4430323.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            3076..3597
                     /gene="sodC"
                     /locus_tag="ACHKYO_RS17735"
                     /old_locus_tag="pgaptmp_003547"
     CDS             3076..3597
                     /gene="sodC"
                     /locus_tag="ACHKYO_RS17735"
                     /old_locus_tag="pgaptmp_003547"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU4430324.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3598..5610)
                     /locus_tag="ACHKYO_RS17740"
                     /old_locus_tag="pgaptmp_003548"
     CDS             complement(3598..5610)
                     /locus_tag="ACHKYO_RS17740"
                     /old_locus_tag="pgaptmp_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU4430325.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNTEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICNSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(5610..6467)
                     /locus_tag="ACHKYO_RS17745"
                     /old_locus_tag="pgaptmp_003549"
     CDS             complement(5610..6467)
                     /locus_tag="ACHKYO_RS17745"
                     /old_locus_tag="pgaptmp_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGU4430326.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(6470..6706)
                     /locus_tag="ACHKYO_RS17750"
                     /old_locus_tag="pgaptmp_003550"
     CDS             complement(6470..6706)
                     /locus_tag="ACHKYO_RS17750"
                     /old_locus_tag="pgaptmp_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HGU4430327.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6907..7341
                     /gene="slyA"
                     /locus_tag="ACHKYO_RS17755"
                     /old_locus_tag="pgaptmp_003551"
     CDS             6907..7341
                     /gene="slyA"
                     /locus_tag="ACHKYO_RS17755"
                     /old_locus_tag="pgaptmp_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HGU4430328.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(7388..7855)
                     /gene="slyB"
                     /locus_tag="ACHKYO_RS17760"
                     /old_locus_tag="pgaptmp_003552"
     CDS             complement(7388..7855)
                     /gene="slyB"
                     /locus_tag="ACHKYO_RS17760"
                     /old_locus_tag="pgaptmp_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HGU4430329.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            8129..9238
                     /gene="anmK"
                     /locus_tag="ACHKYO_RS17765"
                     /old_locus_tag="pgaptmp_003553"
     CDS             8129..9238
                     /gene="anmK"
                     /locus_tag="ACHKYO_RS17765"
                     /old_locus_tag="pgaptmp_003553"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HGU4430330.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            9336..9665
                     /gene="mliC"
                     /locus_tag="ACHKYO_RS17770"
                     /old_locus_tag="pgaptmp_003554"
     CDS             9336..9665
                     /gene="mliC"
                     /locus_tag="ACHKYO_RS17770"
                     /old_locus_tag="pgaptmp_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HGU4430331.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            9724..10380
                     /gene="pdxH"
                     /locus_tag="ACHKYO_RS17775"
                     /old_locus_tag="pgaptmp_003555"
     CDS             9724..10380
                     /gene="pdxH"
                     /locus_tag="ACHKYO_RS17775"
                     /old_locus_tag="pgaptmp_003555"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HGU4430332.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            10509..11783
                     /gene="tyrS"
                     /locus_tag="ACHKYO_RS17780"
                     /old_locus_tag="pgaptmp_003556"
     CDS             10509..11783
                     /gene="tyrS"
                     /locus_tag="ACHKYO_RS17780"
                     /old_locus_tag="pgaptmp_003556"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGU4430333.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            11842..12705
                     /gene="pdxY"
                     /locus_tag="ACHKYO_RS17785"
                     /old_locus_tag="pgaptmp_003557"
     CDS             11842..12705
                     /gene="pdxY"
                     /locus_tag="ACHKYO_RS17785"
                     /old_locus_tag="pgaptmp_003557"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HGU4430334.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(12749..13354)
                     /gene="gstA"
                     /locus_tag="ACHKYO_RS17790"
                     /old_locus_tag="pgaptmp_003558"
     CDS             complement(12749..13354)
                     /gene="gstA"
                     /locus_tag="ACHKYO_RS17790"
                     /old_locus_tag="pgaptmp_003558"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HGU4430335.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     VVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(13459..14961)
                     /gene="dtpA"
                     /locus_tag="ACHKYO_RS17795"
                     /old_locus_tag="pgaptmp_003559"
     CDS             complement(13459..14961)
                     /gene="dtpA"
                     /locus_tag="ACHKYO_RS17795"
                     /old_locus_tag="pgaptmp_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HGU4430336.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNITDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(15573..16208)
                     /gene="nth"
                     /locus_tag="ACHKYO_RS17800"
                     /old_locus_tag="pgaptmp_003560"
     CDS             complement(15573..16208)
                     /gene="nth"
                     /locus_tag="ACHKYO_RS17800"
                     /old_locus_tag="pgaptmp_003560"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGU4430337.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(16208..16903)
                     /gene="rsxE"
                     /locus_tag="ACHKYO_RS17805"
                     /old_locus_tag="pgaptmp_003561"
     CDS             complement(16208..16903)
                     /gene="rsxE"
                     /locus_tag="ACHKYO_RS17805"
                     /old_locus_tag="pgaptmp_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HGU4430338.1"
                     /translation="MSEIKDVIVQGVWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAT
                     AERALPNGETGNV"
     gene            complement(16907..17527)
                     /gene="rsxG"
                     /locus_tag="ACHKYO_RS17810"
                     /old_locus_tag="pgaptmp_003562"
     CDS             complement(16907..17527)
                     /gene="rsxG"
                     /locus_tag="ACHKYO_RS17810"
                     /old_locus_tag="pgaptmp_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HGU4430339.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQEA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(17531..18589)
                     /gene="rsxD"
                     /locus_tag="ACHKYO_RS17815"
                     /old_locus_tag="pgaptmp_003563"
     CDS             complement(17531..18589)
                     /gene="rsxD"
                     /locus_tag="ACHKYO_RS17815"
                     /old_locus_tag="pgaptmp_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HGU4430340.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(18590..>18880)
                     /locus_tag="ACHKYO_RS17820"
                     /old_locus_tag="pgaptmp_003564"
     CDS             complement(18590..>18880)
                     /locus_tag="ACHKYO_RS17820"
                     /old_locus_tag="pgaptmp_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430341.1"
                     /translation="EEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         4805 a         4751 c         4850 g         4474 t
ORIGIN      
        1 cttccgcgcc gccaccgtag aaactttcgg cacgctgtgg gttaagctca gctttgcgct
       61 gcaagcgggc gaccagatcc gggttcgcaa tccagtcacg accaaatgcc accgcatcaa
      121 ttaacccttt gccgatcagc gtttcagctt tttctactgt gtatgcacct gcgccgataa
      181 tcggaccgtg gaaacgggcg cgtacttttt cgcggaacgc atcagtatac ggttcacccc
      241 ccgcccaatc tggttctgac atatgcagat aagcaatgcc gcgtttaccc agttgttcaa
      301 tcagatacag tgcatcggct tcttcattcg ggccgttatc cgtgttctgg aaagtaccga
      361 ttggcgaaac gcgaatgcca atgcgatcgg caccccattc ttcaatcccg gcatcgacca
      421 cttccagtac cagacgtgcg cgattttcca cgctgccgcc gtactgatcg gtacgatggt
      481 ttgaagaagg agaaaggaac tgatgcagca aataaccgtg agcagagtgg agctctacca
      541 gatcaaaacc ggcttcacgc gcgttagcaa tggcctgacg gaaatcattg acgatacctg
      601 gaatctcttc cagttcaagc gcacgcggca tggatgtttc aacacggatc gcctgaccat
      661 tttcatcgcg cagagaagta cgtgttcccg cgctaagtgc tgaaggcgct accggtgcct
      721 gaccgccagg ttgcaggctg gcgtgagaaa tgcgtccggt gtgccacagc tgcacggcca
      781 tatgaccatt ttcagcatga acgccagcgg tgattttttt ccatgcggca atttgctccg
      841 gactatggat gccaggcgca cctgcatatc cttttgcctg ggcagaaatt tgcgtggctt
      901 cactaataat caaaccggca ctggcacgtt ggcgatagta ttccgccatc aacggggtag
      961 gaatgtcacc cggttcaata ctgcgcagac gcgtcagcgg tgccataaaa atacggtttg
     1021 ccgccgtgat cgcgcccact ttcagtgggg aatacagttt ttcagatgac ataaaggctc
     1081 ctggtcgtct ggtgataaaa aatgcctgct aaacggcagg cgtcgcaata atgtttttta
     1141 catgggccag cgcgttttcc agtggctcga aactgcgcga aattttggcc tgcagattcg
     1201 cgccaagcca cagtgcgtaa agcacttgtg cctgttgtag cggttcgcca caaaaggtta
     1261 aacaatggtt ctcacggcca ttttccagcg cctgagagag cagggcgatc acgccgcgag
     1321 cacctttatc catcgcgctg cgcatatctt ctgacagatc gcacacttcg gcagagagtt
     1381 ttactgtcag gcaaccactg atggttccat gttggcaaaa ctggttcagg gtttgctggt
     1441 aataagccag tatgcggtcg cggtagttac cttcgccgga ttgcagcaac tcagtcagtc
     1501 gctggtgata tgcggcgtaa tgacgctcaa gcatggcaac gccaaacgct tctttagagc
     1561 gaaagtagtg atagaaggac cctttcggca cttcagcggt ttttagtaat tcgcttagcc
     1621 ccatcccggt gaatccacgt tgcaggcaaa gttgctcgcc cgtcgccagg agatgttcgc
     1681 gagtatcatg ttcggtgtgt ttgttcatgc gttggagtgt agtagaccag tcggtctagt
     1741 gcaagcaaag ttgctacttt ggacattaac agcttcaata atggtaataa tcttatctga
     1801 ggagggcgaa gtggcggagc aattagagtt ctttcccgtc cagagcccgt gccggggaat
     1861 ttgccagtct gacgaacgcg ggttttgtcg cggctgtttt cgtagtcgtg atgagcgttt
     1921 taactggaat aaaatgagcg atggcgaaaa acaagaggtg ctgcgccttt gtcggcagcg
     1981 attaatgcgc aaattacgcg caaataagcc agcgtcatca gacgaaccga agcaaccatc
     2041 actcttttga acctaatctt gcgtatactc ctctcatatc tgataagagg aagcgattat
     2101 ggttcagcgt attactattg cgccgcaagg cccggagttt tcccgttttg tgatgggcta
     2161 ctggcgattg atggactgga atatgtccgc ccgccagcta gtcagtttta ttgaagagca
     2221 tctggatctc ggcgtgacca ccgtggacca tgctgatatt tatggtggct atcagtgcga
     2281 agcggcgttt ggcgaggcac tgaaactggc acctcacctg cgtgaacgga tggaaatcgt
     2341 cagtaaatgc ggtatcgcga cgaccgcgcg tgaagaaaac gtcattggtc attacatcac
     2401 tgaccgcgat cacatcatta agagcgccga acagtcgcta attaatctcg cgaccgatca
     2461 tctggatttg ctgttaatcc accgaccaga cccgttaatg gatgccgatg aagtggcgga
     2521 cgcgttcaaa catctgcatc agagcggcaa agtgcgtcat tttggcgtat cgaactttac
     2581 gcctgcgcaa tttgccctgt tgcaatcacg tctgccgttt acccttgcca ctaatcaggt
     2641 ggaaatatcc ccggtgcatc agccgttact gctggatggc acgctcgacc aactacaaca
     2701 actgcgtgtt cgtccgatgg cgtggtcctg ccttggtggt ggtcgtctgt ttaatgatga
     2761 ttatttccag ccgctgcgtg atgaactggc tgtggtggca gaggagttaa acgcgggctc
     2821 gattgaacag gtggtttacg cctgggtatt acgtttacca tcgcagccgc tgccaattat
     2881 cggttcaggt aaaattgagc gcgtacgggc agctgtcgaa gcagaaacac tgaaaatgac
     2941 ccgtcaacaa tggtttcgta tccgtaaagc ggcactgggg tacgacgtac cgtaagcact
     3001 tttaggaata gccgccgttc aaaaatgtgt cactggttta cacttattca ggaatgcaca
     3061 atgaacggag gtcctatgaa acgttttagt ctggctattc tggcgctggt tgttgcaacc
     3121 ggcgcacaag ctgccagtga aaaagtcgag atgaacctcg tcacgtcgca aggggtaggg
     3181 cagtcaattg gtagcgtcac cattactgaa accgataaag gtctggagtt ttcgcccgat
     3241 ctgaaagcat taccccccgg tgaacatggc ttccatattc atgccaaagg aagctgccag
     3301 ccagccacca aagatggcaa agccagcgcc gcggaatccg caggcgggca tcttgatcca
     3361 caaaataccg gtaaacatga agggccagaa ggtgccgggc atttaggcga tctgcctgca
     3421 ctggtcgtca ataatgacgg caaagctacc gatgccgtca tcgcgcctcg tctgaaatca
     3481 ctggatgaaa tcaaagacaa agcgctgatg gtccacgttg gcggcgataa tatgtccgat
     3541 caacctaaac cgctgggcgg tggcggtgaa cgctatgcct gtggtgtaat taagtaatta
     3601 cgaggccagc gtaccttgcg gtggtgcttg ctcaagttgc gaaagcgagc agtacagccg
     3661 ccagacaatt gccgccagct cacgggcggc aggttgatga tgacgggcaa ggctgttgca
     3721 aatccgctgt aattcttcaa gtgtggccgc cagtgatttt tgctgaacgc cacgctcact
     3781 catcactccc cgcaacagtg aaatacagtt atcccgtact cgcgataacg gatcggaacg
     3841 cgattcccag tcgcgcaatt gccagacaac atgagaacag ttcagcagca ctacacccca
     3901 gcgtaataac caacggcgag ccagcgcatc ctggctgtta ctcaactgac tgacgtgatg
     3961 ataagtgagc gattcaaatt cgctttcact cagtgttgga tggcggctta gctgatcgac
     4021 aaaatcccgg cgcagcgcgc gaatatggcg gcggctttta cgagcatccg atcctggacg
     4081 cagaatggcg aacgctagcc aagccagcgc aacgccaacg atttttgcca gattatcgtt
     4141 aagaaaatca gcaaaatcat acaccggggg attagtgaca gcgataaacg aacccataaa
     4201 aacaatcagt tgcccccaca gtgcggcaaa tttgggcatc tgcaatttaa gaagctgcat
     4261 tgtcgccagc agtggaaaga gaaacagtaa aaattgccac agatcgctaa tctggaccat
     4321 caggccaaat ttgaccacaa agctgaatag cgaaagtaac accagcgtgc gcatcagcag
     4381 tgacaacgac ttaaacggtg ctgcgacggc ggagtagagt acgcagctaa ttgctgccag
     4441 cgttaatgca ttggcaccgg catcccattg cgatgcaata ctccatgcgc caatcatcat
     4501 caacgtacaa aatgtacgca gcccgctcca catagcttcg gtgttgtcgg tatgacgagc
     4561 cagagcggat gtccgtgcga gtctggcatg atcatctaca ccgctttgca gacgatgtaa
     4621 ttcctgacta ctttgcagat aaaggcggca aaaataacgt agtcgctgcc agaaggcgac
     4681 gtgccggtag tcggcgacgt tggtcgggcg tagcggggcg ataatacgtg cgacggtgta
     4741 agcatctgtt tgcgaactgg cgagcgccgt cagcaattgt tcgagaattt ctcgcgtggc
     4801 acctggcggt gagggccagt tgagcaacat acgtcgcagg ctggagatga cactggtcat
     4861 acgtaattgc tggtggagta gcgcattaag gcgcgcgttt tgctggcgaa aacgatagtg
     4921 gctccagaaa gcctggatac gcagcaaatt catggtcagt atctgcccaa tcaccccctc
     4981 atgtgctgca cgaatggcat cgtttgtttc aggctgccag agtaaactgg catgttccag
     5041 taatcgggcg tgcatgtttt tcaatgcggt taaaagggct gtagcatcgg aactgctagg
     5101 caggatcatc atcatcatgc cgccgcacaa aataccgact atcacctcgc aaacgcgcgc
     5161 ctgagcgata tcccacagct ggctggcctc agtaatatta accatcggaa aggcgatgat
     5221 ggcagccgtg tagcccgcca gttgaaatgc atacgcgacg ttattcgtga agtgcgcaca
     5281 ggcccaggta caaaagccaa gccacgccga catgctcaat agaaaaaacc acggctcatt
     5341 gagcgtatgc ccggcaagaa gcagtgccgc aatggctccc agtaaactgc cagcgatgcg
     5401 tccgaggctt ttgctgatga caccgccaac ggtgggaaag ctaaccactg cagccgaggt
     5461 catcgcccaa tagggttcat ccagattcaa ataataggca accgtcagcg ccagacacat
     5521 ggcaatggta ttgcgtaacg cataacgcca ttgcgccagc gtggccttga accagggcaa
     5581 attgcgcaag gaccatgacg atgcgttcat tattgaccta cggcaatgct acaggtggtg
     5641 cccgacacca gcgtgacatc gcctggaact ttatccagcg caaagcggac gggaacgcgt
     5701 tgggcgagac gaacccaggg gacgttaggt ttcacatccg gaataaggct tgagtcactt
     5761 tcaacgcttt gatcataaat cgcccgaccg atactggaaa cgtgaccccg taacgttttg
     5821 ttatcactat agagggtaat ttgtgctggt gcaccttcgc ggatatggcg caacttggtt
     5881 tcttcaaaat aaccaatgac gtaaaacgag tggctgtcga caagggcaaa cagcggtttc
     5941 ccggtgtcag cgtaatcgcc gatgcgggta gtgaggttag tcacccaacc actcaccggg
     6001 gcgcgaattt ccgtttgcgc cagttgccat tgtgtctgtt tcagcgtggc ctgtgcggca
     6061 ttaacactgg cctgcatcgc tttaacattg aggttagcgg tatcgagttc ttcggcagaa
     6121 ataaaatttt gtgagagatg acgacgacga ttcgcttcgt tattggcttt tgcgaggtca
     6181 gattgcgctt tcgccagctg agcctgtgcg ttcagctcgg cgatctgaaa gggcgtttta
     6241 tcgatcgtga gcagaagatc ccccgcgtta accagttggt tgtcttttat attcagctca
     6301 acaatacgtc ccgacacctg tggggtgata gaaacctttt ctgcgcgtat tttcccgtcg
     6361 cgtgtccacg gagattgcat gtaataattc cacagccacc atccggcaag aaccgcaact
     6421 atcgctacaa tgattgttga gaaatactta attgttttaa ttgacatagt taccacgcaa
     6481 tcagtattgc cagagcaagg caaacgcaaa tcgcaaacag cgataaatcc attaacaagg
     6541 gatgccagat gtcaccggcg tagatccagc cacgaagcag gcggtgtacg acaagccaga
     6601 tgacgaatcc aaacgcgaag gctttgaaaa acggaggaaa atagacggac gcaccgaaca
     6661 ccagatcttg taagggcaat cctgttgcat tgagcataaa cttcacaaag tagattcctt
     6721 tacgaccgaa tatattgcgt tagattaata aatattcttt aagtgcgaaa aatttacgcg
     6781 caatttctga aaaatatgtc tgacggtaac caaatgcagc aatacatttg ttttagcaat
     6841 acaattgctg cacactattc taaaagccgc ataatatctt agcaagctaa ttataaggag
     6901 atgaaattgg aatcgccact aggttctgat ctggcacggt tggtgcgcat atggcgtgct
     6961 ctgatagacc atcgcctgaa accgctggag ttaacacaaa cccattgggt tacgttacac
     7021 aatattcatc agttacctcc agaccagtcg caaattcaac tggcaaaagc gattggcatc
     7081 gagcagccat cactggtccg tactctggac caactggaag aaaaagggtt aatttcgcgt
     7141 caaacttgtg ccagcgatcg tcgggctaaa cgtattaaac tgacggaaaa ggcagagcca
     7201 ctgatcagcg aaatggaagc tgttattaac aaaacccgcg cggaaatatt acatggcatc
     7261 tccgcagagg aactggagca actgattacg ctcatcgcaa aacttgagca taatatcatt
     7321 gagttacagg ccaaagggtg aaatgaaggg ggcgtgtggt cagtaacctg accacacgta
     7381 aacttattta gcgcggagaa acggtcacct gactgccatt gctggccagt acgacacgtt
     7441 ggcccggaga gaaacgagtg ttgccttgtt tctgtaccac catgatggta ttaccatcgt
     7501 ctttacgaat ttccagctcg acaccctgcg ttttgttcat tgcactctgt acgccctgac
     7561 cagctacgcc acctgcaaca gcgcctgctg cagtagccag agaacgcccg gttccgccac
     7621 caacagtatt ccccaggaaa ccaccaagaa cagcaccgcc aattgcaccg ataacgttgg
     7681 aatcatcacc gccctgaatc tgtaccggac gtacgttaac gatggtgcca tagctgacat
     7741 tctgtacttg tttcgcttca gaagcggtat aaacatcccc tgacagggtg tcgttattaa
     7801 cacaaccgac aagagacaga cctaccattg aaacaaccaa tacgcgttta atcattgaaa
     7861 aatctcctgt tcaccatgaa acgccacgca agcatccctc atggtcaaag tatatggcat
     7921 atgtgaatcc atggttacat gttctaccaa atatgagcaa atcataatga aagtaggatc
     7981 aaccaattat aaacaaaaca ttcatgatga ttattgattt gaagataatg ttttactacc
     8041 aacggtaaaa atcggtcatt ccgcatggta ttcacgaaaa gtttatgttt gagtggtgcg
     8101 caaacgtcag attgaaaaag gatgaactat gaaatcgggc cgctttattg gcgttatgtc
     8161 aggcaccagc cttgatggtg ttgatgttgt gttggcgaca atagatgaac accgggtcgc
     8221 acagctggca agcttgagct ggccgatccc ggtgtctctg aaacaggctg tactggatat
     8281 ttgccagggc cagcagctta cactttcgca gtttggacag cttgatactc aactcgggcg
     8341 actttttgct gatgcggtca atgccttgct taaggaacaa aacctgcagg cgagagatat
     8401 agttgcgatc ggctgtcacg gtcaaaccgt ctggcatgaa ccgacgggcg tggcaccaca
     8461 cactttacag attggcgata acaatcaaat tgtggcacgc accggaatta cggttgtcgg
     8521 tgattttcgc cgtcgcgata ttgccttggg aggacaaggc gcaccgctgg tacctgcgtt
     8581 ccatcatgcc ttgctggctc actcaaccga gcgacgaatg gtgctcaata ttggcggcat
     8641 cgccaatctg tcactgctca ttcctgggca gccggttggc ggctacgata ccggtcctgg
     8701 taacatgctg atggatgcct ggatctggcg tcaggccggt aaaccttacg ataaagatgc
     8761 cgagtgggca cgggcgggta aagttattct cccactgctg caaaatatgc tcagcgaccc
     8821 atatttctcg caacctgcac cgaaaagcac cggacgcgaa tactttaact acggctggct
     8881 ggagcgccat ttacgccatt ttccgggtgt tgatccccgt gatgttcagg ccacactggc
     8941 agaactcacc gccgtgacca tttctgaaca agttttgttg agcggtggct gcgaacgatt
     9001 gatggtatgt ggtggaggta gtcgtaatcc gctactcatg gcgcgtctgg cggcattact
     9061 gccaggcaca gaagtcacca ccaccgatgc cgttggcatt agtggcgatg acatggaagc
     9121 attggctttc gcctggcttg cctggcggac gctggcggga ttaccaggaa atctgccttc
     9181 cgtcactggc gcaagccagg agacggtact gggggctatt ttccctgcta acccgtgata
     9241 tcagagttaa ctgaattttt cttaccgcgt ctgccgttac acttggaacg ttagggaggg
     9301 cgtattgccc tccagaccag gaaagtcttc gggaaatgtc tatgaaaaaa ctgttactga
     9361 tctgtttgcc ggtgcttctc accggctgta gcgcgttcaa tcaactggtt gagcacatgc
     9421 aaaccgctac gctggaatac cagtgtgatg aaaaaccgtt gacggtcaaa ctgaataatc
     9481 cgcgccagga agtcagtttt gtttacgata atcaactact gcatctcaaa cagggcattt
     9541 cagcgtctgg tgcacgttac actgacggaa tctatgtttt ctggtcgaaa ggcgatgaag
     9601 cgactgtcta taaacgcgac cgcatcgtat tgaataactg tcagttacaa aatccacagc
     9661 gttgagattt ttccaggggc ggcgcacaat agcgtcaccc actgacaatc cgtaaagaaa
     9721 accatgtctg ataacgacga attgcagcaa atcgcgcatc tgcgccgtga atacaccaaa
     9781 ggcgggttac gccgtcgcga tcttcccgcc gatccattaa ccctttttga acgttggctc
     9841 tctcaggcgt gtgaagccaa actggcagac cctaccgcga tggtggtcgc taccgtggat
     9901 gaacatgctc agccttatca acgcatcgtt ttactcaaac attacgacga aaaaggcatg
     9961 gtgttttaca ccaacctcgg tagccgtaaa gcacatcaaa ttgaaaataa tccgcgcgtt
    10021 agcctgctgt tcccgtggca tacccttgaa cgccaggtga tggtgatcgg taaagcagaa
    10081 cgactttcga ctctcgaagt gatgaaatat tttcatagcc gcccgcgtga tagccagatt
    10141 ggtgcatggg tttcgaagca gtccagtcgc atttctgccc gcggtatcct tgaaagtaaa
    10201 ttcctggagc tgaagcagaa gtttcaacag ggcgaagtgc cattgccgag cttttggggc
    10261 ggttttcgcg tcagccttga acagattgag ttctggcagg gcggtgagca tcgcctgcat
    10321 gaccgctttt tgtaccagcg agaaaatgat gcgtggaaga ttgatcgtct tgcaccctga
    10381 aaagatgcaa aaatcttgct ttaatcgctg gtactcctga ttctggcact ttattctatg
    10441 tctctttcgc atctggcgaa aagtcgtgta ccggcaaagg tgcagtcgtt atatacatgg
    10501 agattttgat ggcaagcagt aacttgatta aacaattgca agagcggggg ctggtagccc
    10561 aggtgacgga cgaggaagcg ttagcagagc gactggcgca aggcccgatc gcgctctatt
    10621 gcggcttcga tcctaccgct gacagcttgc atttggggca tcttgttcca ttgttatgcc
    10681 tgaaacgctt ccagcaggcg ggccacaagc cggttgcgct ggtaggcggc gcgacgggtc
    10741 tgattggcga cccgagcttc aaagctgccg agcgtaagct gaacaccgaa gaaactgttc
    10801 aggagtgggt ggacaaaatc cgtaagcagg ttgccccgtt cctcgatttc gactgtggag
    10861 aaaactctgc tatcgcggcg aacaactatg actggttcgg caatatgaat gtgctgacct
    10921 tcctgcgcga tattggcaaa cacttctccg ttaaccagat gatcaacaaa gaagcggtta
    10981 agcagcgtct caaccgtgaa gatcagggga tttcgttcac tgagttttcc tacaacctgt
    11041 tgcagggtta tgacttcgcc tgtctgaaca aacagtacgg tgtggtgctg caaattggtg
    11101 gttctgacca gtggggtaac atcacttctg gtatcgacct gacccgtcgt ctgcatcaga
    11161 atcaggtgtt tggcctgacc gttccgctga tcactaaagc agatggcacc aaatttggta
    11221 aaactgaagg cggcgcagtc tggttggatc cgaagaaaac cagcccgtac aaattctacc
    11281 agttctggat caacactgcg gatgccgacg tttaccgctt cctgaagttc ttcaccttta
    11341 tgagcattga agagatcaac gccctggaag aagaagataa aaacagcggt aaagcaccgc
    11401 gcgcccagta tgtactggcg gagcaggtga ctcgtctggt tcacggtgaa gaaggtttac
    11461 aggcggcaaa acgtattacc gaatgcctgt tcagcggttc tttgagtgcg ctgagtgaag
    11521 cggacttcga acagctggcg caggacggcg taccgatggt tgagatggaa aagggcgcag
    11581 acctgatgca ggcgctggtc gattctgaac tgcaaccttc ccgtggtcag gcgcgtaaaa
    11641 ctatcgcctc caatgccatc accattaacg gtgaaaaaca gtccgatcct gaatacttct
    11701 ttaaagaaga agatcgtctg tttggtcgtt ttaccttact gcgtcgcggc aaaaagaatt
    11761 actgtctgat ttgctggaaa taatgcatta agtggaaagg gggagtgaga aatcactccc
    11821 cctggttttt atacagggaa catgatgaaa aatattctcg ctatccagtc tcacgttgtt
    11881 tatggtcatg cgggtaacag tgcggcagag tttcctatgc gccgcctggg cgcgaacgtc
    11941 tggccgctga acaccgttca attttctaat cacacccaat acggcaaatg gactggctgc
    12001 gtgatgccgc ccagccattt aaccgaaatt gtgcaaggca ttgccgccat tgataaatta
    12061 cacacctgtg atgccgtgtt aagtggctat ctgggatcgg cggagcaggg tgaacatatc
    12121 ctcggtatcg tccgtcaggt gaaagccgcg aatccgcagg cgaaatattt ttgcgatccg
    12181 gtaatgggtc atccggaaaa aggctgtatc gttgcaccgg gtgtcgcaga gtttcatgtg
    12241 cggcacggtt tgcctgccag cgatatcatt gcgccgaatc tggttgagct ggaaatactc
    12301 tgtgagcatg cggtaaataa cgtcgaagaa gcggttctgg cagcgcgtga actcattgcg
    12361 caagggccac aaattgtgtt ggttaaacac ctggcgcgag ctggctacag ccgtgaccgt
    12421 tttgaaatgc tgctggtcac cgccgatgaa gcctggcata tcagccgtcc gctggtggat
    12481 tttggtatgc gccagccggt aggtgttggt gatgtgacga gcggtttact gctggtgaaa
    12541 ctgcttcagg gggcaacgct gcaggaggcg ctggaacatg tgaccgctgc agtctacgaa
    12601 atcatggtga ccaccaaagc aatgcaggaa tatgagctgc aagtggtggc tgctcaggat
    12661 cgtattgcca aaccagaaca ttacttcagc gcaacaaagc tctgaaatat ctcatttcgg
    12721 cccacaacga cctgtgggcc gatggatatt actttaagcc ttccgctgac agcgcgtctt
    12781 gtacttccgg acgttcagcc atacgttgca taaatgctgc aatgtgctct aacccttcca
    12841 gattcagttt caccgcgtat gcccagcgca gaaccgtaaa cagataggca tcagcaattg
    12901 taaatctttg cccgcagatc cagtgctcat ccttcagtgc ctcgttcaca tattgcagct
    12961 tcttctccag ctgcgcgcga actgtcggtt tgtactcttc cggtgtatca gggcgaaaca
    13021 gaggtgtgaa acctttatgc agctcggtgg cgatgtaatt cagccattcg atggttttat
    13081 agcgggaaat actgtttacc ggtgccagca actggcggtc ggggacgctg tcggcaagat
    13141 actgcataat cgctacgcct tccgtcagca aagtaccgtc atccagcagc aatgcaggca
    13201 cctgcccctt agggttaacg acaaagtaat cgtcaccgtt ttcgagacgt tttttcatta
    13261 aatccacact gacgagggta aaatcctttc cgctctcacg cagggtgata tgggaagcga
    13321 gagagcaggc acccggtttg tagaacaatt tcatcggtaa ctccttttgt ctgtggttta
    13381 aagtatggta gtgcgttgct gaacaaaaaa aagccgctaa cgaaaagtta gcggcttgta
    13441 aaagagtttc cctgaatttt acgctacagc agctttcgcc gctttgtctg cagcgtcatc
    13501 ctgcgtcata cggtgcagtt taggcgcggt cagcagcatc agtactgcaa taatggcagt
    13561 agcgacacca atctgcaaga atacgcgacc ataaacttcc agtgacatca gcgggtcggt
    13621 aatgttatcc ggcacagcca tcatacccgc aacataacca ccaatcaggt ttgcaccggc
    13681 agtggtcagg aaccagctac ccataatgaa gcccatcaga cgctgcggaa cgagttgtgc
    13741 aaccattgcc agacccagac cagagatcat cagttccccg atgctctgca ggccatagct
    13801 tgcgaccagc cagcttacag acacgatacc agcgtcagac gcgaatttcg cacccagcgg
    13861 cagaatcagg aacgcaccag aacacatcac catgccgatt gcaaacttgg ttggcatcgg
    13921 cagggtatcg cccatcttgt tatagatagc ggccagaatc ggactaccga tgatgatcca
    13981 gaacgggttc agtgcctgat actgctcagg ttctacggcc agacccagaa tggagtgctc
    14041 aacgttacga atcgcaaaga agttcagtga cgttggcatc tggctgtaca gcacgaagaa
    14101 gataatggct tcgagcatca ggatgaaggc aacgatcatt ttacgacgcg cagcaccttt
    14161 catcgcgaag gcttctttac cgaagataac cacgataccg aaggcaacaa cgcccagcgc
    14221 catacgcgca acttcctgat tgtgcagcag ccaggtggcg atagcgatca gtgccacaac
    14281 accaataatg gtcagcagca ggttacggta gttgataggc tcgaagtctg gttttgaacc
    14341 gtactgttta acccagcgtt ggcagaaggc gaagttaacg atagtgatca gcaggcctac
    14401 aacgctcaac gcaaacgcaa cactccagcc gtatttcgcg gccagccacg gcgtagcaat
    14461 catagagaag aaagagccga tgttgacgga catgtagtac atggtgaatg caccgtccag
    14521 acgcgggtcg tttttctcat agcatgtaga aagcagagaa gacgggttag ctttaaacag
    14581 gccgttaccg accgcaatag ccgccatacc catataaacg ataccggcgt cgtgaccaga
    14641 ccaggcaacc agagcataac caatcgccag cacaatagcg ccgagcataa ttacgcgttt
    14701 agtacccagt accttgtcac ctaaccagcc gccgatagcg accagaccat aaaccagggc
    14761 actaaaggaa gagaaaaggg tgattgaatc cgcttcagac atacccagtt gtttaaccag
    14821 gtaaacagcc ataattcctt gtaggccgta ataaccaaaa cgttcccata actcaatcga
    14881 gaagatgaga tagaacgcct tcggttgttt gaaagcgttc aaactgacgc tttcagttgg
    14941 tttttggttt gcagtggaca cttttacctc tttttttaca tcccatatta acgggggtgc
    15001 tttctcgcgt tatgtctaaa aaacatacgc gcaattgtta tagtgggagg ggaaacggca
    15061 ggtaatgttc actatcttgc actttcagac aagtcctttg taataatctg ttacatataa
    15121 ctgaggcggg gaattcaacc attccgggaa atgttatcca gcgttaaatt tttcaaaaaa
    15181 cgtaactgca gattttttca ctgcactgag agggggggtg atggcttaac ggcgatatgt
    15241 tctgctattt agtgttttta ctagtgagat agtccagttt ctgaaaaata gccagtgtaa
    15301 tgttttgtag gtcaatattt gttgcgtgtg taaatttaaa tgattttaac ctgattcttg
    15361 caatgttttt acattgcgac agcatcgtga tcttgatcac gaatgaatag aaaatttcgc
    15421 tattaaaaaa acaattaacc tgcatttttg gttatctgca aacggattac ctgatgataa
    15481 cgctaagcaa tggcatcatt cacgttaaaa agatgtttta aaagaggata aagaaaggtt
    15541 atcaatgggg taatcggtgt tacccctttt cttcagatgt caactttctc tttgtattca
    15601 caaagatctt caataataca agagccacag cggggcttgc gggcaatgca ggtataacgc
    15661 ccgtgcagga tcaaccaatg gtggcagtcg actttaaact ctgccggaac cactttcagt
    15721 agcttttctt ctacctgttc gacgtttttc cccggcgcaa attgagtacg attacaaacg
    15781 cggaaaatgt gcgtgtcgac agcaatagtc ggccagccga atgcagtgtt taatacgacg
    15841 ttggctgttt tacgacctac gccgggcagg gcttcaagcg cagcacgatc ttccggaacc
    15901 tcgccattat gctgctccag caagatacgg caggttttga tgatattttc tgctttgctg
    15961 ttataaagcc caatcgtttt gatataggtt ttcacccctt caacgcccag ttcaagcatc
    16021 gctgcaggcg tattcgccac cgggtagagt ttcgccgtcg ccttattaac actgacatcg
    16081 gtcgcctgag cggaaagcag tacggcaatc agcaattcaa aaggcgaact gaaattaagc
    16141 tcggtggtgg gatgaggatt gttctcacgc aggcgagtga ggatctccag gcgttttgct
    16201 ttgttcatca gacattccct gtttcaccgt ttggcaatgc acgttcggca gttgcttcag
    16261 cacggcgctt tttcattctt tcatcaatca ggtattttcc tgccagcatc agtcccaggc
    16321 caataaatgc acctggaggc agcatcgcca gcaggaaagg ggagtcggtg tggaaaattt
    16381 ccacgcgtaa tacttttgcc cagctaccta acagcgcatc tgcaccgtca aacaatgtgc
    16441 cattgccgat aatttcgcgt agtgaaccca gcacgaacat ggcgcaggtt gcgcccatac
    16501 caattgaaaa gccgtccagt gccgaaagcg ccggaccttt tttggcggcg aaggcttcag
    16561 cgcggcccac aacgatacag ttagtgacaa tcagcggaat aaaaatccct aatgattgat
    16621 acaggccaaa ggcgtaggcg ttgatcagca tctgtacagc gctgaccacc gaggcgatga
    16681 tcatcacgta aatgggaatg cggatctcgg ctggcgtcca gtgacgcagc gtcgaaatgg
    16741 tcaggttggt cagcgtcagt accagcgtag tcgcaagtcc taaacccaga gcgttagtgg
    16801 cagtggacgt gaccgccaac agaggacaaa ggccgagcaa ctggaccagc gcagagttgt
    16861 ttttccacac cccctgaaca ataacgtctt taatttcgct cacgctttat tctccacagg
    16921 caggaagttg agaaagttgt gccggtaacg tctgagcgta caatccggcg cgttttaccg
    16981 cattaaccac cgcgcgggga gtaatcgtcg cgccggtgaa ctggtcgaaa tcaccaccat
    17041 ctttcttcac cgcccagtgc gcatcatccg caccactgat ttttttaccc gcaaaatggg
    17101 tgatccagtc agaaaggcgc agttcgattt tatcgccaag ccctggcgtt tcgtggtgct
    17161 ctgtcacgcg cgtgccaagt accgtgccgt taaaatcggc tcccaccagc agctgaatcg
    17221 caccggaata gccatctggc gcggttgctt ccagaacggc ggctaccggt ttgtcatcct
    17281 gtttggcgat gtaaacccga tgctcacctt tacctaactc tggcgcagtt accagatagc
    17341 aactctgtgc cagcgcattg ttatagcgtt cggctggcag cacctgatca aataatgcct
    17401 cttgttgcag actggcctgt tcagcaatcg tcgttttggt catctggttg atggccgcag
    17461 ttaaccctgt tgaacccgct gcaaatagcg ctaacgtaat gccgtgtttt cggatagttt
    17521 tcagcatggt ttaccctttg cgatggccgt agacgcgcgg acgcgtgtag taatcgatca
    17581 gaggaaccgt gatgttcgcc agcaggacgg caaaagccac gccgtcagga tagccgccga
    17641 aactgcggat caaccagact aataagcccg ccagcgcgcc gaaaataaga cgaccacgat
    17701 tggtcgtaga agcggtaacc gggtcagtca aaataaagaa tgcgccgagc atggtcgcac
    17761 cagacagcag atgaatttgc ggtgctgcca gtgtttctgg tgagaacaac cagcccaacg
    17821 ttgcgcataa cgccagcgtt actaagaagc tgaggggaat atgccagcga atcgctttct
    17881 gccatagcaa ccacaggccg ccagccagcc aggcgagatt tacccattgc caaccagcgc
    17941 ccgccagaat accgctgtag atcggatatt gcataatctg ttcaaccgaa tgaccggcac
    18001 ggacagaggt tttaaatgta tccagcggtg tcgcctgact aatgccatca atacctaagc
    18061 gtagtgtgtt catatcacca ccactggcgg tatgtccgct gaaaataacc tgtatggcgt
    18121 cgataaaacc agggatgttg accgcaattt catgtggcgg taaccagctg gtcatctgca
    18181 ccgggaagga gatcagtaag accacataac caatcattgc cggattaaac gggttttgcc
    18241 ccagaccgcc atacaactgt ttagcgatga tcaccgcaaa caccgtaccc agcacgacca
    18301 tccaccatgg cgcgaggggg ggaatactta ccgccagcaa taagcctgtc agcaatgctg
    18361 agttatcttt caacgttgcg gctaccgact gcttgcgtag tttgagtacg agagcttcgg
    18421 ctaacagagc actaaccgat gccaacagga tctgaacgag agtaccccaa ccaaaaaacc
    18481 acagttgcgc tgcgattcct ggcacggctg cgagcaacac caacagcata atgcgcgatg
    18541 tctggcgctg gttatgggta taaggggagc tagctattct gaataccatt tagtcctcgt
    18601 ttacaacctt ctgctgggcg gcttttttgg cctgagcgcg ggcaatagcc gcggcaactg
    18661 ccgctttgcg cggatcaacc tgttcttctg gttctgcgtt agtctgttgc tgttccagct
    18721 tgcgcgcttt ggcacgggca atagcggctt cgacggcggc tttgcgcgga tcaacctgtt
    18781 cttctggttc cgcattagcc tgttgctgtt ccagtttgcg cgctttggct cgggcaatag
    18841 cggcttcgac ggcggctttg cgcggatcaa cctgttcttc
//
LOCUS       DAZGAN020000092        18468 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGAN020000092 DAZGAN020000000
VERSION     DAZGAN020000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.092"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(111..374)
                     /gene="cedA"
                     /locus_tag="ACHKYO_RS17825"
                     /old_locus_tag="pgaptmp_003565"
     CDS             complement(111..374)
                     /gene="cedA"
                     /locus_tag="ACHKYO_RS17825"
                     /old_locus_tag="pgaptmp_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HGU4430342.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            557..2818
                     /gene="katE"
                     /locus_tag="ACHKYO_RS17830"
                     /old_locus_tag="pgaptmp_003566"
     CDS             557..2818
                     /gene="katE"
                     /locus_tag="ACHKYO_RS17830"
                     /old_locus_tag="pgaptmp_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HGU4430343.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2865..3623)
                     /gene="chbG"
                     /locus_tag="ACHKYO_RS17835"
                     /old_locus_tag="pgaptmp_003567"
     CDS             complement(2865..3623)
                     /gene="chbG"
                     /locus_tag="ACHKYO_RS17835"
                     /old_locus_tag="pgaptmp_003567"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HGU4430344.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVVRFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDSSHRRERSLEVMCHPAFVDNLIRKSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3636..4988)
                     /gene="celF"
                     /locus_tag="ACHKYO_RS17840"
                     /old_locus_tag="pgaptmp_003568"
                     /gene_synonym="chbF"
     CDS             complement(3636..4988)
                     /gene="celF"
                     /locus_tag="ACHKYO_RS17840"
                     /old_locus_tag="pgaptmp_003568"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGU4430345.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5093..5935)
                     /gene="chbR"
                     /locus_tag="ACHKYO_RS17845"
                     /old_locus_tag="pgaptmp_003569"
     CDS             complement(5093..5935)
                     /gene="chbR"
                     /locus_tag="ACHKYO_RS17845"
                     /old_locus_tag="pgaptmp_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HGU4430346.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5943..6293)
                     /gene="chbA"
                     /locus_tag="ACHKYO_RS17850"
                     /old_locus_tag="pgaptmp_003570"
     CDS             complement(5943..6293)
                     /gene="chbA"
                     /locus_tag="ACHKYO_RS17850"
                     /old_locus_tag="pgaptmp_003570"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HGU4430347.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6344..7702)
                     /gene="chbC"
                     /locus_tag="ACHKYO_RS17855"
                     /old_locus_tag="pgaptmp_003571"
     CDS             complement(6344..7702)
                     /gene="chbC"
                     /locus_tag="ACHKYO_RS17855"
                     /old_locus_tag="pgaptmp_003571"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HGU4430348.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAELLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7787..8107)
                     /gene="chbB"
                     /locus_tag="ACHKYO_RS17860"
                     /old_locus_tag="pgaptmp_003572"
     CDS             complement(7787..8107)
                     /gene="chbB"
                     /locus_tag="ACHKYO_RS17860"
                     /old_locus_tag="pgaptmp_003572"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HGU4430349.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8406..8744)
                     /gene="osmE"
                     /locus_tag="ACHKYO_RS17865"
                     /old_locus_tag="pgaptmp_003573"
     CDS             complement(8406..8744)
                     /gene="osmE"
                     /locus_tag="ACHKYO_RS17865"
                     /old_locus_tag="pgaptmp_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HGU4430350.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            8946..9773
                     /gene="nadE"
                     /locus_tag="ACHKYO_RS17870"
                     /old_locus_tag="pgaptmp_003574"
     CDS             8946..9773
                     /gene="nadE"
                     /locus_tag="ACHKYO_RS17870"
                     /old_locus_tag="pgaptmp_003574"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGU4430351.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10003..10890
                     /gene="cho"
                     /locus_tag="ACHKYO_RS17875"
                     /old_locus_tag="pgaptmp_003575"
     CDS             10003..10890
                     /gene="cho"
                     /locus_tag="ACHKYO_RS17875"
                     /old_locus_tag="pgaptmp_003575"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HGU4430352.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10850..11425)
                     /gene="ves"
                     /locus_tag="ACHKYO_RS17880"
                     /old_locus_tag="pgaptmp_003576"
     CDS             complement(10850..11425)
                     /gene="ves"
                     /locus_tag="ACHKYO_RS17880"
                     /old_locus_tag="pgaptmp_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HGU4430353.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11628..12113)
                     /gene="spy"
                     /locus_tag="ACHKYO_RS17885"
                     /old_locus_tag="pgaptmp_003577"
     CDS             complement(11628..12113)
                     /gene="spy"
                     /locus_tag="ACHKYO_RS17885"
                     /old_locus_tag="pgaptmp_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HGU4430354.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12443..13411)
                     /gene="astE"
                     /locus_tag="ACHKYO_RS17890"
                     /old_locus_tag="pgaptmp_003578"
     CDS             complement(12443..13411)
                     /gene="astE"
                     /locus_tag="ACHKYO_RS17890"
                     /old_locus_tag="pgaptmp_003578"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HGU4430355.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDHRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13404..14747)
                     /gene="astB"
                     /locus_tag="ACHKYO_RS17895"
                     /old_locus_tag="pgaptmp_003579"
     CDS             complement(13404..14747)
                     /gene="astB"
                     /locus_tag="ACHKYO_RS17895"
                     /old_locus_tag="pgaptmp_003579"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HGU4430356.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14744..16222)
                     /gene="astD"
                     /locus_tag="ACHKYO_RS17900"
                     /old_locus_tag="pgaptmp_003580"
     CDS             complement(14744..16222)
                     /gene="astD"
                     /locus_tag="ACHKYO_RS17900"
                     /old_locus_tag="pgaptmp_003580"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HGU4430357.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHARTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16219..17253)
                     /gene="astA"
                     /locus_tag="ACHKYO_RS17905"
                     /old_locus_tag="pgaptmp_003581"
     CDS             complement(16219..17253)
                     /gene="astA"
                     /locus_tag="ACHKYO_RS17905"
                     /old_locus_tag="pgaptmp_003581"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HGU4430358.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17250..>18468)
                     /gene="astC"
                     /locus_tag="ACHKYO_RS17910"
                     /old_locus_tag="pgaptmp_003582"
     CDS             complement(17250..>18468)
                     /gene="astC"
                     /locus_tag="ACHKYO_RS17910"
                     /old_locus_tag="pgaptmp_003582"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HGU4430359.1"
                     /translation="SQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDFA
                     GGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFCN
                     SGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAPL
                     PADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNAL
                     LIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVGT
                     HGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRGL
                     GLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDRF
                     AAACEHFVSRGSS"
BASE COUNT         4652 a         4974 c         4386 g         4456 t
ORIGIN      
        1 atagctcgtc aaaggcagct ttttgttaca ggtggtttga atgaatgtag caacgaaatg
       61 cagaatttca ggtcatgtaa ctcccggcaa aaccgggagg tatgtaatcc ttactcagtc
      121 acttcccctt cctggcggat ctgatttgcc caacgttggg cagattcagg cacagtaaac
      181 gccggtgagc gcagaaatga ctctcccatc agtacaaacg caacatattt gccacgcagc
      241 atccagacat cacgaaacga atccatcttt atcgcatgtt ctggcggcgc gggttccgtg
      301 cgtgggacat agctaataat ctggcggttt tgctggcgga gcggtttctt cattactggc
      361 ttcactaaac gcatattaaa aattagaaaa actgtagttt agccgattta gcccctgtac
      421 gtcccgcttt gcgtgtattt cataacaccg tttccagaat agtctccgaa gcgggatctg
      481 gctggtggtc tatagttaga gagttttttg accaaaacag cggccctttc agtaataaat
      541 taaggagacg agttcaatgt cgcaacataa cgaaaagaac ccacatcagc accagtcacc
      601 actacacgat tccagcgaag cgaaaccggg gatggactca ctggcacctg aggacggatc
      661 tcatcgtcca gcggctgaac caacaccgcc aggtgcacaa cctaccgccc ctgggagcct
      721 gaaagcccct gatacacgta acgaaaaact taattctctg gaagacgtac gcaaaggcag
      781 tgaaaattat gcgctgacca ctaatcaggg cgtgcgcatc gccgatgatc aaaactcact
      841 gcgtgccggt aaccgtggtc caacgctgct ggaagatttt attctgcgcg agaaaatcac
      901 ccactttgac catgagcgca tcccggaacg tattgttcat gcacgcggat cagcagctca
      961 cggttatttc cagccatata aaagcttaag cgatatcacc aaagcggatt tcctctcaga
     1021 tccgaacaaa atcaccccag tatttgttag gttctctacc gttcagggtg gtgctggctc
     1081 tgccgatacc gtgcgtgata tccgtggctt tgccaccaaa ttctataccg aagagggtat
     1141 ttttgacctt gttggcaata acacgccaat cttctttatc caggatgcgc ataaattccc
     1201 cgattttgtt catgcggtaa aaccagaacc acactgggca attccacaag ggcaaagcgc
     1261 ccacgatact ttctgggatt atgtttctct gcaacctgaa actctgcaca acgtgatgtg
     1321 ggcgatgtcg gatcgcggca tcccccgcag ttaccgcacc atggaaggct tcggtattca
     1381 caccttccgc ctgattaatg ccgaagggaa ggcaacgttt gtacgtttcc actggaaacc
     1441 actggcaggt aaagcctcac tcgtttggga tgaagcacaa aaactcaccg gacgtgaccc
     1501 ggacttccac cgccgcgagt tgtgggaagc cattgaagca ggcgattttc cggaatacga
     1561 actgggcttc cagttgattc ctgaagaaga tgaattcaag ttcgacttcg atcttctcga
     1621 tccaaccaaa ctgatcccgg aagaactggt gcccgttcag cgtgtcggca aaatggtgct
     1681 caatcgcaat ccggataact tctttgccga aaacgaacaa gcagcattcc atccaggtca
     1741 tattgttcct ggtctggact tcaccaacga tccgctgttg cagggacgtt tgttctccta
     1801 taccgataca caaatcagtc gtcttggtgg gccgaatttc catgagattc cgattaaccg
     1861 tccgacctgc ccttaccaca acttccagcg tgatggcatg catcgtatgg ggatcgacac
     1921 taacccggcg aattacgaac cgaactcgat caacgataac tggccgcgcg aaacaccgcc
     1981 gggaccgaaa cgcggcggtt ttgaatcata tcaggaacgc gtggaaggaa ataaagttcg
     2041 tgagcgcagt ccatcgtttg gcgaatatta ttcccatccg cgtctgttct ggctaagtca
     2101 gacgccattc gagcagcgcc atattgtcga tggtttcagt tttgagttaa gcaaagtcgt
     2161 tcgtccgtat attcgtgagc gcgttgttga ccagctggcg catattgatc tcactctggc
     2221 ccaggcggtg gcgaaaaatc tcggtatcga actgactgac gaccagctga atatcacccc
     2281 acctccggac gtcaacggtc tgaaaaagga tccatcctta agtttgtacg ccattcctga
     2341 cggtgatgtg aaaggtcgcg tggtagcgat tttacttaat gatgaagtga gatcggcaga
     2401 ccttctggcc attctcaagg ccctgaaggc caaaggcgtt catgccaaac tgctctactc
     2461 ccgaatgggt gaagtgactg cggatgacgg tacggtgttg cctatagccg ctacctttgc
     2521 cggtgcacct tcgctgacgg tcgatgcggt cattgtccct tgcggcaata tcgcggatat
     2581 cgctgacaac ggcgatgcca actactacct gatggaagcc tacaaacacc ttaaaccgat
     2641 tgcgctggcg ggagacgcgc gcaagtttaa agcaacaatc aaggtcgctg accagggtga
     2701 agaagggatt gtggaagctg acagcgctga cggtagtttt atggatgaac tgctaacgct
     2761 gatggcagca caccgcgtgt ggtcacgcat tcctaagatt gacaaaattc cggcgtaaat
     2821 atggataagg tcacgtcccc tggggagtga ccttaccacc ttacttacac atcaagataa
     2881 ctacccaacc gataaccacg ctcagcaatt gcatatttca acgacgctga tgtcagcact
     2941 tccagctccg ttaaccgtgg gaaacagtaa gcactcttgc gaatcaaatt atcgacaaac
     3001 gccggatggc acatgacttc cagcgagcgt tccctgcgat ggctggaatc atccagcact
     3061 tgcaggaaca gtgcctcgct aatctcttca ccataaaacg cgctactgaa tccctgcgaa
     3121 ctgcgcagat tagccggtaa atcaccagcg ttagatagcg gctgacggtc gatgcgcaac
     3181 gcaatcccct cttcagccgc aaacctgaca acaatcggga aaatctgcgg gaacatatgc
     3241 acatgatgat ggctatcaag atgcgtaggt ttgcgtccaa atagctcaat gaaacgcaaa
     3301 tactgactgg caagctcctg agtaatttct tccagcggta aagcatcttc ttctgccaac
     3361 tgccagatcc attttcccag cacaccatcg cgggttaacc ccggcatagc tgtcagtggc
     3421 ttgcccatag taaggacaaa gtgcatccct atggccagac tcggttcatc acgactcaac
     3481 tgcaccgcat ggtcaatagc ctgcccattc accagcgccg tcgtcgatgt gacaatccca
     3541 ttgcgacagg cctcgataat gccgtagttc tgacctttgc ttaagccaaa atcatcggca
     3601 ttaacaatca gtaagcgttc cataatcagc ctcggttaat gtgctttttt aagctctgcg
     3661 atgcagtcgg caaaatttgg cagccatttc tcgtgcgcca gaatcatctc gcgtgccagc
     3721 agctcagcat cgcgatcgga atgcaccaac ggactaaggt ttagcgccag taacacatcg
     3781 ttaaattctc cgctaagtgc ggcgttgctg gcagcaatct cgaagccttt aatggtgtga
     3841 atcagcccca tcactttatc atcgaaatgc gtaatgcgcg gatgtggcgt cgcgccatcg
     3901 cgccccagct tacaggtcat ttctaccgcc caatctgccg gaatattatc aatatgccca
     3961 tgatgcggga tattaacgta atgttcagct tgcttgtcgt tgtagatagc gttgatcact
     4021 tcgcacgctg catcagagta ataagcccca ccgcgctgtt ccagttcttt cggcttaact
     4081 ttcaactccg gatttttata cagctcaaaa agttgtttct cgactttctg tactacctgt
     4141 gctcgtgcgc cgcctttgta gtattcgccc atttcaatag ccagcatctc tttctgcttg
     4201 aagtaataca gcagataaga acatggcagc agatttaacg aacgaattaa gccctcacta
     4261 aatggcagat cgaaaatatt ttttacagag gacgctttta actgccctga cgccacacca
     4321 tcaagcaatt cggcaaagcg cgacttgcca tttaccagca catccttaat gaacaccata
     4381 tggttgaggc cgaacagatc gatagataaa tcatcgctgt ctttcagcat cagaacatcg
     4441 cgaataaaca tcttcatgcc gatcggaata ttacacacgc cgataaagcg tttaaatccg
     4501 gtatgacgat aaacggcttc agtgaccatt cccgccgggt tagtgaagtt aatcacccat
     4561 gcattcggac aaagttcttc gacatctttt acgatgtcaa aaatcaccgg aatggtacgc
     4621 agacctttaa acagaccgcc cgcgccgttg gtttcctgac caagataacc atgacttaat
     4681 ggaatacgtt catccagttc gcgcgccggt aattggccta cgcgcagttg ggtagtaacg
     4741 aaatcagcat ctttcaatgc ttcgcggcga tccagcgttt tataaagctt catcgggacg
     4801 ccagcgttat caatcatccg ttggcagagt tcaaaaatga tatccagttt ctctttacca
     4861 tcttcgacat ccaccagcca taattcgctg accggcaatt cgtgataacg cttaataaat
     4921 ccttccagta actccggggt atagctgctc ccgccaccaa tagtgacgac ttttaatttc
     4981 tggctcataa tttctccctt cagtacagaa tactgatatc tggcatatct gcccccccgg
     5041 acataaataa tccagcaaca gtacagatat gtgaattgtc aggtataacg acttactgat
     5101 taaattcagt caatttctta cgatagctct taggcgtaaa ggacgttaat ttcttaaacg
     5161 ttttaataaa caaactcggg ctactataac cggcctcaaa cgcaatatcc gtcacggaat
     5221 agttggtcat ttccagttgt tttttggcaa aattaatacg gatctcatta ataatctgca
     5281 ttggtgtttt gccataatat cgttgagtcg ctcgcgtcaa atattcctgt gatttggctg
     5341 acaacgtcac catattctcc agcgccgatt cactaaactg ctctttatca tgcatctttt
     5401 ctaccgtact tttcagccac tgcggtacat catcaatcac ctgttcttcg cggtaatgac
     5461 gtaaacggtt aatgacataa aaagtaacca tctcaacaaa ctcttccagc cctgtttcgc
     5521 ggaaattcaa tgaagaaatc actgtttcca cataggtgag aaaagcgtta ttggtccggt
     5581 atacctgcga agcgacaaag caataaggca acaacggcag gtaatgctgc tcaaaaaagc
     5641 gtttactgat cccaacgttc aatatacgcg tggcaccaaa ctcataaaaa ctttgatggt
     5701 gcgaacctaa cggaataaaa acaaaatcgc cccgttccag taacacgcgc ttaccgttaa
     5761 tctcctggaa ataacgcccg gttaatacca gagtaaattc ataatagtcg tgctgatgca
     5821 gcccgctgat actctcagtt ttgttataga taaacacatg gaagtttttg ccattaaaca
     5881 actgctgttc tcgggcagtg gcaatttccg gcgcgttaat cactggctgc atcatcgact
     5941 ccttatgcct tcagtttttc atgaagctca attaattcag taatcagttc acgcgcaagc
     6001 atggacgtca ttaaatgatc ctgagcgtgg accagcacca gactcacttt catcttacct
     6061 tcgcccgcat cgccttcaat cagtttcgtc tgtaccagat gcgcttcatt caatgccatc
     6121 cgtgactgat ccatcatggc ttttgctgca gcaaaatcgc cctgcttcgc ctgtttcagt
     6181 gccgcatacg ccaggctgcg cgcttgtccg gagttgatga taagccccat caccacttct
     6241 tccagctctt cagcttccgt ttgcgtatcg ggaatgttat cgagatccat catacatcgt
     6301 tcctctctat tcttaccggc acgattaccc gtaccggcat cgattaaaat ttcagggcat
     6361 tagcgatatc ttcttcgctc tcttctttat caatcgcgtt ttgtgctttg ttagccacca
     6421 caacaaaggg cagataaatt aacgttgcga taccaaggtt gaagagtgcg accagcaatg
     6481 cggcgacgct accgttggtg ttaaagaagg ctcccagacc ggttggcatg gtccacggtg
     6541 caatattggt caccggagga atgatgccca tgtagtacgc tgcgagggtg attgccgcca
     6601 gaatcggttg taccagtaca aacgggataa acatcaccgg gttcatgata attggcagac
     6661 caaacagaat cggttcgtta atctggaaga tgccggacgg cagcgccagt tttgccacct
     6721 gacgataatc agcacggcga gaggcgataa agatagccag gatcaggcct aaagtcgcac
     6781 cactgccccc aaggaaaata aaggagtcca gcatcggctt ggcccagata tggaaggtct
     6841 taccggctgc cagcgccgct tcgacggaac catattgctg ataggtcgcg atattttcca
     6901 gcgcccacgg cgtcataatg ccgttgtcca gtgcggtcag cgccagcgcg ccatgaatac
     6961 cgaagaacca gagcagtgga acaaagatca cataggccca gcccaccacg ctacccaacg
     7021 atgccagtgg ggttgagatg gtatccataa tgatctgatg gaagttggtg ccccaggtat
     7081 tcaacgccca ggcaataatc cccatcacgg aaagaataat aaagccggga attaatgccg
     7141 agaaggaacg cgatactgaa gcaggtacgc tgtcgggcag tttaatgacc caattgcggc
     7201 ggacaataaa ggtaaacatt tctgccacca ccaggccaat aataatcccg gagatgatat
     7261 tcgccccacc taaccagttt gcgccaaccg cataggcctc accgacacta tatggggtga
     7321 cggtcataaa tgctgcaacg gataacaact cagccgccag cgcatcgact ttacgctctt
     7381 ctgccagcgc catgccaata aagaacggtg ccattaaaga cattatccct aatgttccgt
     7441 tatatacgtt gccgccaata cctttcagac cattaagtgt ttcaatggtt gaggcgtcga
     7501 ggcgaatacc taaggaataa aaaaacgacc cctccccaaa gcttagaaaa acgttgttaa
     7561 ttaatacaaa catggccccc gcaagggtta acggcattaa gcgaataaag ccatttttga
     7621 ttgcattaac gtgtggctgc tttcctattt taactgcaaa agggaggagt accttttcaa
     7681 gcgatgcaat aacattactc atagaaaaat acccttaaaa accgcaattt aaatattgcg
     7741 gtattgattt atgaaataac tctttgacgg gaaaatttaa aataaattaa tttgctgcgg
     7801 cttttttaat cgctgcaacc gcagccttaa gcacgcctaa accatcgact ttgccataaa
     7861 gcagcgagtc aattacttca accggtttgt tgggtaacaa acgctggatt tcgggcaaca
     7921 tataagcaat ctgcggccct aataacacga catcggcatt ctgacctttt tcaccagcca
     7981 gtgtttccgg aaatgcttca ataatgaccg gaacttcata tttttctgcc tgtgcgcgca
     8041 tttttgatac cagtaaagag gtagacatgc ccgcagaaca aaacagataa atgtgtttct
     8101 tttccataaa actgccctca tcgacgatta tctgtcagcc agacactccg caagccttaa
     8161 cctgcttcca tgctctgggt aacttgcgaa accaacatga ctttttttgt ggctgaaagg
     8221 agtatacggt atagacctga gcaatagtat ttctttgacc ttataaattg tttctccttg
     8281 acctgtggtt atgaccctct tcacatttcg ggcaaataat tcgcgaagat aattaagaac
     8341 tcaggccaaa aaaaaccggc gcaatggccg gtttccgttg ttactcaagc tttcagacga
     8401 attgattact tcgcagcctg tggatcagtg tcgtattcag cacaggtctg ataaccggag
     8461 ttgatgacat gtccggtatc atctaacgcg acaaagtagg tttctgcttt accatcacgt
     8521 tgacccagga tgtaggtctg gcaagtaccg cgagcatgga tcatgctcac ttcagacgaa
     8581 ggtttacccg caatttgtgc aacctgcgcc cggctcatgc cttttttgac gtctttcacc
     8641 acaggctgta caaactggtc tttagtacga tcataagccg tacaacccgc cagcatggtt
     8701 aataccgccg ctgcactcag aattcctgcc atattcttgt tcatattccg tcctcttgtt
     8761 tatcagcgtg ttagataagc ctggaataca ttgggcgctt tttcaagccc gtgaacgaaa
     8821 cagctccgct ttcagaggat tcctgtatga cgttttaacc accattcagc ccgctgtcgc
     8881 ttgtcgtttc agtagcaacg ggttagcttt aaggaagttt tgtcttttct gtctggaggg
     8941 gttcaatgac attgcaacaa caaataataa aggcgctggg cgcaaaaccg cagattaatg
     9001 ctgaagagga aattcgtcgt agtgtcgatt ttctgaaaag ctacctgcaa acttatccgt
     9061 tcattaaatc actggtgctc gggatcagcg gcggtcagga ctccacgctt gccggaaagc
     9121 tgtgccagat ggcgattaat gagctgcgcc aggaaactgg caacgaatca ctgcaattta
     9181 ttgccgtacg cctgccctat ggtgttcagg ccgacgaaca agattgccag gatgccattg
     9241 cctttattca accggatcgc gtattaaccg ttaatatcaa gggcgcggta ttggctagcg
     9301 agcaggcatt gcgggaagca ggcattgaac tgagcgattt tgtccgtggc aatgaaaaag
     9361 cgcgtgagcg gatgaaagca caatatagca ttgcgggtat gaccagcggt gtcgtggtgg
     9421 gcaccgatca tgcagcagaa gccattaccg gattcttcac taaatatggt gacggcggta
     9481 cggacattaa tccgctgtat cgtctcaaca aacgtcaggg taaacagtta ctggcggcat
     9541 taggttgccc ggaacacctt tataagaaag cgccaacggc cgatctggaa gatgatcgcc
     9601 cttctctgcc agatgaagtg gcactcggcg tgacctatga caatatcgac gactatctgg
     9661 aagggaaaaa cgtacctgaa caggtcgcca gaacaataga gaactggtat ctgaaaaccg
     9721 aacataaacg ccgtccgcca attaccgttt tcgatgattt ctggaaaaag taataatttg
     9781 cacctattgg attgtgcgaa aaagagtaat ttgttcacgc cggatgcggc gtgaacgcct
     9841 tattcgacct acaaaactat gcaaattcaa tacattgcag gagtcgaata ggcctgacag
     9901 gcgtagcacg tcagacggtg taacctttgt catcaacccg cctctttttt aatcgcctcc
     9961 cgcctgttac actggataga taaccagcat tcggagtcaa cagtggtacg gcgtttaact
    10021 tctccgcggc tcgaatttga agctgcggca atttatgaat atcccgaaca tttacgttca
    10081 ttccttaatg acttacccac ccgaccaggg gtgtatctgt ttcatggcga aagtgacacc
    10141 atgccgctct atatcggcaa aagcgttaat atccgcagtc gggtgctttc tcatttacgc
    10201 accccggatg aagccgccat gctacggcaa tcccggcgaa ttagctggat ctgcaccgca
    10261 ggcgaaatcg gcgctctgct ccttgaagcg cgattaatca aagaacaaca gccgctgttt
    10321 aataaacggt tgcgccgcaa tcgccagctc tgtgccctgc aattaaatga aaagcgcgtc
    10381 gatgtggtgt atgccaaaga ggtggatttt tcacgagccc ccaacctgtt tggcctgttt
    10441 gccaataggc gcgcagcttt gcaagcattg cagagcatcg ctgatgaaca aaaactttgt
    10501 tatggcctgt tgggactgga accgttaagt cgcggtcgtg catgttttcg ttcagcgcta
    10561 aaacgttgcg ccggagcatg ctgcggtaaa gagagccatg aggaacatgc gctacgcttg
    10621 cgccagtctc tggagcgttt gcgggtggtg tgttggcctt ggcaaggtgc ggtggcactg
    10681 aaagaacagc acccggaaat gactcaatat catattattc aaaactggct gtggctgggg
    10741 gcggttaatt cgctggaaga agcgacaacg ttaattcgga cacccgccgg gtttgatcac
    10801 gacggttata aaattctttg taagccgctg ctttccggta actatgaaat tactgaactt
    10861 gatccggcga atgaccagcg agccagttga tttcactgaa caacagttta ccttgcggtt
    10921 gtaaaagccg cagggtgtgc ctgccatcga accagcacgc gccctggtcg gtagtgagca
    10981 atttatcgcc cagttgccac gccccgttaa tcacaaacac caccccgccg cgtgagccaa
    11041 aggtagtaaa cgtacgttcg gcaattctga ctttcgcctt acagacatcc aggcgtgtca
    11101 tgatgttgaa atccatcgac atctgccctg ccgtcagttt cgcttttacc acctggtccg
    11161 ctgcaaaggc aaaaggctgt aacggtttta aggtatggtt aaagcggtct gcgctttcaa
    11221 ggaacatctc gccgccttcc agcaacgtca ctatcctttc catgccggga aacaaagaaa
    11281 actccccatt cgccgcgatg gatgcaatgc tggcacgcca gtaaaaatca cgttttgccg
    11341 gtgggaaagt gcaaatttcg cgcgtttcac cggcagcgtt tcgccacaga ttcaccgaca
    11401 ttttacgcat atcaaagtat tccatactcg ctcccggccc gttcttttta ttgttatcgt
    11461 tcaggtaacc gtggatggtt atcggcaact cgggtgattt acgttagtgg tgatcaggct
    11521 ttttttacaa ctgtttgaga agagaaaaga aaaccgccga tcctgtccac cgcattactg
    11581 caaggtagtg gacaagaccg gcggtcttaa gttttttggc tgaaagatta ttcagcagtt
    11641 gcaggcattt taccttttgc cgctggacgt tctgtcagac gcttctcaaa attagcatta
    11701 aattgctttt tctgttccgg cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc
    11761 gccagcatgt tagctttgcg ctgttcttcc atttttgcga tctgcgcttc agcttttgct
    11821 ttatcgaagg tatcgctggc aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga
    11881 cgtttcatct ggtcacgctg gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg
    11941 gtcaggttca ggtctttgaa catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc
    12001 atcatcggct tcgcgtcagc cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta
    12061 gccgcgccaa gagccagggt agaggcaaca aacagtgcag ttaatttacg catattctat
    12121 atccttcctt tcagttattt attacggctt tcttaagtag cgtgccgtgt tgacgagatt
    12181 aactttactg actttagcgt caattaatca gagcaacggt aaaacaatga aagtgtaaaa
    12241 aacacttttg cgccaattat ggagaaaaaa agaaaatttg atggagagtg atgagagaat
    12301 aatacaacac gatgattttg cagagattat gaagaactat accggatgac tggtgataaa
    12361 taaagcaaat aaccaggatt aatctgtatt aatttataag aaagcaactt aatacccgca
    12421 gaatgatttc tgcgggtaag tattagctta ttttttcgag cattaatccc gcgcgtaatc
    12481 ccaacgctac caacggatta gggaataaca catactctac atcatgggtt acggtaaaac
    12541 gttcctctcc gtcctgcgcc agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt
    12601 cacttgccat atgcatttcg aaggacggcg agtggcgagt aatttgcgaa accacccgat
    12661 aacggagcgg cggtgttctc acgataccga cactctcacc agatagcagc gcagcaattg
    12721 cgctggcagt tactgcaaac tggcgatgat cgttttgccc aaagggcaac gctttgccaa
    12781 gttccagcgt acaggccagc gcgccaaaat gtctggcgct gaaatgggta aacgtaccac
    12841 caggttcctg atggaacacc agcgcctcca gccccgccgc acccagccac gtcagaaatt
    12901 tctcgtccca gggaatgtcg cgttgcggta atacaccgaa ctgcggatgc aaggagccac
    12961 gaattgcggt atgtagatca aggtgccagc gcacagattc tttgcccagg tcataaaaat
    13021 cttccaggca ctgttccagt tcgcgcgccc gacaggtttc tccgctttca gcaaatagct
    13081 gccaacgacc gccaaacatt cgattcatat cgctatggca ataacgtttc ccttgcttca
    13141 gcgcaggagg attcccgagg atcaccagca accgccaacg taacgggatc tcgccgtgag
    13201 atatcgcgcc aagcaacgcg tccagcatct ccacaggtgc cgtctcatta ccgtgtattc
    13261 ccgctgaaat caccagtgcg ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat
    13321 cgcccagcca gcgccagcga acgccgttga tttctcgctc ggtgataacc ggttttttac
    13381 ccgttaaggt cagagcaaga aaattatcca ttgccccctc cctcgcgctg gaacggataa
    13441 accgaaccga gattcagtaa ttggctcaat acatccagtg cttcccgccc ttcgcgcagc
    13501 aattgcgggt cggccagatc ggcagcagta aggcgatcgc ggtagtaacg atccacccag
    13561 tcattgagcg cattaaacag cgtatcgttc atcatcaccg ccggattcac cgcccggcgt
    13621 tcttcttctg tcaataccac ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg
    13681 ctttcacgta aatcaaagac ttttagttcg ctaatcgggt tgtcagcggc aaggagttca
    13741 ttgagataac cccatactcc ggcgtgttcc cgacactcct gaggcagcac caacatcatg
    13801 gaaccatcat cgcggctcaa cagttggctg ttaaacagat aggtagacac cgcatccgac
    13861 acggaaacct gagttgccgg aacttctatc gccataaaac cgttcacccg cgcacgcagg
    13921 tttgccagta actgtgactg gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg
    13981 ttactcacgg caatcacgtc attatgaaaa acgccctggt cgataacgtc cgggttttgc
    14041 tgggcgaaaa tcacctgttg gggattcacc tgattcagcc ttgccaccgc ctcgctggct
    14101 tcgcgagtct gtcgcgccgg ataacgggaa ggccgggtat cattgccttc ttctcgcccg
    14161 tagacaaaaa gttgcatacc cggctcaccg taatgaccgc cgagacgatt gtggtttgcc
    14221 gccccctcat caccgagcaa cgctacctgc ggcaacgccg aatgaacgct aaatttctct
    14281 tcgtcgttaa aaatcgcttt taaaagtgat tcggtgacgt gcgcttccag cgagcggtga
    14341 aatttattgt tcaggttggc aacggtgaga tgcactttgc catccagcgt atcggcagat
    14401 ggcgcgatcg ttgccgcatt ggctacccac attggcgaag cggaactgac gctggaaagc
    14461 cagtgcggtg cctggcgtgc aactttttcc agtacctgct catcgctacc actgaatccc
    14521 aactgacgca gcaccggaat aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat
    14581 cccgcatcgg caagggcttt cattttcagt aagccctgtt ttgccgcctg tcgcgggtta
    14641 gacacctgaa aacggtgacg ggtagaggct tcattaccaa acgacaggcc agcgtaatga
    14701 tgcgtcagcc ctaccagccc gtcgaaatta acttcccagg cgttcatcgc accacctcat
    14761 cggaaaaatc cagcccgggg ttaagcgttg cgggcaaagt taacgagtcc gactccaggc
    14821 tcgccatagg ccatgcgcag taatctgcgg cataccaggc gctggggcga tggttaccgg
    14881 atgcaccaat gccgccgaat ggcgcggtac tggcagcacc agtaagcggt ttgttccagt
    14941 taacaatccc cgcccgcgcc tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa
    15001 ccagaccgca agagagtccg aagcgagtgt tattcgccat tagaatcgct tcctcgaaag
    15061 aatcataacg ccagacgcgc aataacggtc cgaacacctc ttcatctggt acgccagcaa
    15121 cgcctgtcat ttcaatgatc cccggcgtca gcaacgatgt ctctgattgt aataagcgcg
    15181 gcgcaagcag ggttcgtccg cccatcgctt ccagttgctg ccaggcagta accacctgct
    15241 gtgcggcctg ttcagaaatc agcccgccaa taaacggctg cggttcgtca tcccagttgc
    15301 ccggcgttaa tcgctggctg acggcaacca gacgagcaag aaacgcatcg ccctgcgctc
    15361 cgcttttgag caataaacgg cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg
    15421 actgaatggt cagatggaca gccgcgtcga tatccgccac ctcatcgata attagcggat
    15481 tattaccgcc catctcaagg gcgagaattt tctccggctg accggagagc tggcgatgca
    15541 gctggtagcc ggtattggcg ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac
    15601 tcagcgcctg acccgtttca cgcccgccct gcaccaggtt cagtacgccc ggcggcaagc
    15661 cagcctgctg ccataaacgc attaccgctt cgccactcca cggtgtcagt tcgctgggtt
    15721 taaagataat ggtgttacct gccagcaatg ccggaacgat atgtccgttc ggcaaatgac
    15781 cagggaaatt atacggccca aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg
    15841 ccgcaccgtc cggcatttca ctacgctgct cgccggtacg agcgtgatac gccttaattg
    15901 atatcgcgat tttattgatc atcgccgtca cttcggttgc cgcttcccag cgcggcttgc
    15961 cggtttctcg ggcaatgatt gcggttaatt cgcctttatt cctttccagc aatccggcaa
    16021 agcgttcgac aacgacctga cgttcagcca atgagagccg cgcccagcgc ggaaacgccg
    16081 cacgggctgc ccgacaagcc tgctcgacct gagcggcatc ggcatcattg ccttgccata
    16141 acacctcgcc cgataccgga ttacgcttca cacgcgatgc gccctggccc gttatccagt
    16201 caccgttaat ccataaagtc atgctgtttt ctcctctgcg cacaggcgca ccagacgaac
    16261 gcgatcccct gcgtggcatt tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc
    16321 ggttgccgga tcggtacgcg ccagcaccac gcggaaatgg tgataatttt cattggcgac
    16381 caggcaggcc ggaaaatcgc cctgcgcagg ctgcccttct gccacttcca ccagccgact
    16441 tttacgaatg gcgcgcacgc ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat
    16501 gtcgatatag ttgcggtaac gaaaaccttc tttctccagc accgcgcggg caggcgcggt
    16561 ttgcggatgt acctgaccga tgacgtcctg ggcttcctgg gataaaaagt gggtatagat
    16621 cggatgtttc ggcatcagtt ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc
    16681 ggcgcggcta aaatccatcg aaaagaagcg tttaccgagg ctttgccaga acggtgaata
    16741 gccgtgttcg tcaatcaccc cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg
    16801 aaaagccgcc ataaacataa agcgcgattt cgacagcaaa tagccgttgc cctctttgcg
    16861 ccagtccggg tcgagaaaca gcgtgcacag ctcgctgctg ccggtgtgat cgttactgag
    16921 aaacagcgtc ggcaatgcgt tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc
    16981 gacgcgatag ttgtaccagg gatcgttcag cccaaccgcc acctcaatgg cacaaatccc
    17041 cgccacggtg cctgtctcgc tatcttccag cacgaacaca tagccctgct cacttttggg
    17101 cagttcgcct tgccaggttt tgattgccct ttcgatacgc gccgaaagcg tggcttcatt
    17161 ggcgggaagc gacgtcaggc cgccgcccgt tttgctggca agctgcatca gcgccgagac
    17221 atctgatcgc tcaacgggac ggatgaccat catgatgaac ctcggctaac aaagtgttcg
    17281 caagcagctg caaagcgatc cagtccggtc gtcacctctt cttcgctgac attgagcgca
    17341 ggcgcaaaac gcaccacgtt gccacccgca atcagtacca tcacgcctgc tttcgccgct
    17401 tcctgagaga tctgtttcgc ttgcccggcg taatcggcat tcagtacaca gccaatcagc
    17461 aaacctaagc cgcgaacttc actgaacaaa ccatagcggt gattaatagt attaagacgc
    17521 tcaacaaacc agtcgtgacg ctgtttaacg ccattaagca tctctggtgt gttgatgagc
    17581 tccagcactt tgcctgccac cgccgaggcc agcggattac cgccataggt ggtgccatga
    17641 gtgccaacgg tcatcacgcg ggcgcactct tcggttgcca acaacgcacc gaccgggaaa
    17701 ccgccgccca gcgctttggc ggtagttaac agatcaggcg tcacgccgta gtgcatatag
    17761 gcatacagtt ccccggtgcg cccgacgccg gtttgtactt catcaaaaat caacagcgca
    17821 ttgtggcggt tacacaattc acgcagacct tgtaaaaacg cgttgctggc tggcaccaca
    17881 ccgccttccc cctggatggg ttcgacaatc accgcacagg tagagtcgtc aatcagcgcg
    17941 ctggcagagt taatatcgtt atatgcagca tgacgaatat ccgccggcag tggcgcaaaa
    18001 tcctgtgaat aggctggctg cccacccgca ctgacagtaa acagcgtgcg accatgaaac
    18061 gcatttttga acgccacgat gccgctctta tggctgccgt agcggtcgtg agcgaattta
    18121 cgcgccagtt ttagcgccgc ttcgttggct tccgcaccgg agttacaaaa gaagacgcga
    18181 tcggcaaacg tggcgtcgat caattttttc gccagtcgca gtaccggctc gttggtgtaa
    18241 ccgttgccgg tatgccagaa cttactcgcc tgttcgttca gcgcttcacg cagttccgga
    18301 tgcgcatggc ccagcgcgtt caccgcaatg ccacccgcga agtcgatata ctctttcccc
    18361 tgctgatccc acaagcgcga accttcgcca cgtaccggta taaagggtgc cggagcgtaa
    18421 acaggtatca tccattcatc aaagttttca cgcgtaattg gctgagac
//
LOCUS       DAZGAN020000093        18436 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGAN020000093 DAZGAN020000000
VERSION     DAZGAN020000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.093"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            133..751
                     /locus_tag="ACHKYO_RS17915"
                     /old_locus_tag="pgaptmp_003583"
                     /pseudo
     CDS             133..751
                     /locus_tag="ACHKYO_RS17915"
                     /old_locus_tag="pgaptmp_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            854..1357
                     /gene="yfbM"
                     /locus_tag="ACHKYO_RS17920"
                     /old_locus_tag="pgaptmp_003584"
     CDS             854..1357
                     /gene="yfbM"
                     /locus_tag="ACHKYO_RS17920"
                     /old_locus_tag="pgaptmp_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HGU4430360.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1424..2881)
                     /gene="nuoN"
                     /locus_tag="ACHKYO_RS17925"
                     /old_locus_tag="pgaptmp_003585"
     CDS             complement(1424..2881)
                     /gene="nuoN"
                     /locus_tag="ACHKYO_RS17925"
                     /old_locus_tag="pgaptmp_003585"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HGU4430361.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRVLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2888..4417)
                     /gene="nuoM"
                     /locus_tag="ACHKYO_RS17930"
                     /old_locus_tag="pgaptmp_003586"
     CDS             complement(2888..4417)
                     /gene="nuoM"
                     /locus_tag="ACHKYO_RS17930"
                     /old_locus_tag="pgaptmp_003586"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HGU4430362.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4581..6422)
                     /gene="nuoL"
                     /locus_tag="ACHKYO_RS17935"
                     /old_locus_tag="pgaptmp_003587"
     CDS             complement(4581..6422)
                     /gene="nuoL"
                     /locus_tag="ACHKYO_RS17935"
                     /old_locus_tag="pgaptmp_003587"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HGU4430363.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6419..6721)
                     /gene="nuoK"
                     /locus_tag="ACHKYO_RS17940"
                     /old_locus_tag="pgaptmp_003588"
     CDS             complement(6419..6721)
                     /gene="nuoK"
                     /locus_tag="ACHKYO_RS17940"
                     /old_locus_tag="pgaptmp_003588"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HGU4430364.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6718..7272)
                     /gene="nuoJ"
                     /locus_tag="ACHKYO_RS17945"
                     /old_locus_tag="pgaptmp_003589"
     CDS             complement(6718..7272)
                     /gene="nuoJ"
                     /locus_tag="ACHKYO_RS17945"
                     /old_locus_tag="pgaptmp_003589"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HGU4430365.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7284..7826)
                     /gene="nuoI"
                     /locus_tag="ACHKYO_RS17950"
                     /old_locus_tag="pgaptmp_003590"
     CDS             complement(7284..7826)
                     /gene="nuoI"
                     /locus_tag="ACHKYO_RS17950"
                     /old_locus_tag="pgaptmp_003590"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HGU4430366.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7841..8818)
                     /gene="nuoH"
                     /locus_tag="ACHKYO_RS17955"
                     /old_locus_tag="pgaptmp_003591"
     CDS             complement(7841..8818)
                     /gene="nuoH"
                     /locus_tag="ACHKYO_RS17955"
                     /old_locus_tag="pgaptmp_003591"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HGU4430367.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8815..11541)
                     /gene="nuoG"
                     /locus_tag="ACHKYO_RS17960"
                     /old_locus_tag="pgaptmp_003592"
     CDS             complement(8815..11541)
                     /gene="nuoG"
                     /locus_tag="ACHKYO_RS17960"
                     /old_locus_tag="pgaptmp_003592"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HGU4430368.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11594..12931)
                     /gene="nuoF"
                     /locus_tag="ACHKYO_RS17965"
                     /old_locus_tag="pgaptmp_003593"
     CDS             complement(11594..12931)
                     /gene="nuoF"
                     /locus_tag="ACHKYO_RS17965"
                     /old_locus_tag="pgaptmp_003593"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HGU4430369.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLHSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12928..13428)
                     /gene="nuoE"
                     /locus_tag="ACHKYO_RS17970"
                     /old_locus_tag="pgaptmp_003594"
     CDS             complement(12928..13428)
                     /gene="nuoE"
                     /locus_tag="ACHKYO_RS17970"
                     /old_locus_tag="pgaptmp_003594"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HGU4430370.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13431..15233)
                     /gene="nuoC"
                     /locus_tag="ACHKYO_RS17975"
                     /old_locus_tag="pgaptmp_003595"
     CDS             complement(13431..15233)
                     /gene="nuoC"
                     /locus_tag="ACHKYO_RS17975"
                     /old_locus_tag="pgaptmp_003595"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HGU4430371.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15327..15989)
                     /gene="nuoB"
                     /locus_tag="ACHKYO_RS17980"
                     /old_locus_tag="pgaptmp_003596"
     CDS             complement(15327..15989)
                     /gene="nuoB"
                     /locus_tag="ACHKYO_RS17980"
                     /old_locus_tag="pgaptmp_003596"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HGU4430372.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16005..16448)
                     /gene="nuoA"
                     /locus_tag="ACHKYO_RS17985"
                     /old_locus_tag="pgaptmp_003597"
     CDS             complement(16005..16448)
                     /gene="nuoA"
                     /locus_tag="ACHKYO_RS17985"
                     /old_locus_tag="pgaptmp_003597"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HGU4430373.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17079..18017)
                     /gene="lrhA"
                     /locus_tag="ACHKYO_RS17990"
                     /old_locus_tag="pgaptmp_003598"
     CDS             complement(17079..18017)
                     /gene="lrhA"
                     /locus_tag="ACHKYO_RS17990"
                     /old_locus_tag="pgaptmp_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HGU4430374.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4616 a         5115 c         4736 g         3969 t
ORIGIN      
        1 agccactgta aaagatactt tttgtataga tctaacaacc gtaataaaac aaacgctcat
       61 aactcattat aaataatgat aaaataacgc cttttattga taatttacat atgaaaaaaa
      121 tttccttttg ttatattatt tctgttttca ctccctctga ttatttttta ccaaccctgg
      181 gtcactgcat tatcgccaat gccccgacac gtaagtcctg agcaattaga aaaaacagtt
      241 cgttatctta cacaaactgt gcatccacgt aatgccggca atattgataa tctgaatagg
      301 tccgtagagt atataaaaga ggtctttgtc agtagcggtg ccagagttac cgcgcagggc
      361 gtccccgatt ggctattacg actctgcgcc tggaagccag aattaccctt acccggcaat
      421 gtcctggctt tatcccgatc ggggagactt tattgccgta gtcggcagaa tgcaggatat
      481 caacgccgtt cgtcaggtaa aatcggcatt gttgtcatcc cgggatttat ctgtttattc
      541 tatgaatgcc ccagggttta ttcccggtat tgatttctct gatcacctta ataattggca
      601 acacgatatt cccgccgtaa tgattactga caccgctttt tatcgtaata aacaatacca
      661 cttgcccggt gataccgcag acagattgaa ttatcagaaa atggctcagg tggtggatgg
      721 tgttataaac ttgttataca acagtaaata ataaaaattt ctcgaggcgg gtccagaaaa
      781 agcgacatgc acattttatt ttccctcgtc tgttttaaga tgcgaaacat gaaattcaat
      841 ggaggaaaaa taaatgggaa tgattggcta ttttgcagaa attgactcag aaaagattaa
      901 ccaactgctt gagtctactg aaaaaccctt aatggacaat attcatgaca cgctttcagg
      961 attacgccga ctggatattg acaaacgctg ggatttttta cactttgggc tgacaggcac
     1021 ctctgctttt gatcccgcga agaatgatcc gctgagtcgt gccgtgcttg gtgaacacag
     1081 cctcgaagat ggcatagatg gcttcctggg attgacctgg aatcaggagt tagccgcgac
     1141 aatcgaccga ctggaatcac tggatcgaag tgagctgcgt aaacagtttt ccattaagcg
     1201 actcaatgaa atggagattt atccgggagt gaccttttca gaagagttag aggggcaact
     1261 ctttgcctcg atcatgcttg atatggaaaa gctgattagc gcgtatcgac gtatgctgcg
     1321 acaggggaat catgcgctga cggttattgt tgggtgatgg tgtcagggca ccaaaaaaaa
     1381 ccgccgaaca tgtcggcggc tttctgactt acaaactgat agcttacatc agcggcattg
     1441 ctaaccgcac aatgctaatc agcggttgtg gccatacacc cagcaccagt accaacagtg
     1501 cagaaatcag caccacgata ccgcccgcgc tgtactgcca gtttgacggt gcatcgcgac
     1561 ccggttgttc cggtgcgtgc aggtacaggc tcaccgccac gcgcaggtag tagtagaggc
     1621 cgattgccga accgacaacc acagcaccca ccagccacca caagtgtgcc tggacaccga
     1681 ccgccagcac gtagaactta ccgataaagc ccagcgtcat cgggataccg gccagagaca
     1741 gcatcatcac cgtcatcacc gccgcgagga tcgggcgatg ccagaacaga ccgcggtaag
     1801 agaacaggga atcagcatcc gggccacggt acgggctgga catcaggctg accacgccga
     1861 acgcgccgag gctgctgaac agataaccgg ccaggtaaac tcctaccgct tccatcgaca
     1921 tctcgcccgt ttgcagcgca atcagcgcca ccagcagata gccgaggtga gagatagatg
     1981 agtaaccgag cacacgtttg atattggtct ggctcagcgc catcaggtta ccgaagatga
     2041 tagaggcaaa ggcgataatc gccagcacta cccgaatcgc ttcgctgtca cccaccggtg
     2101 cgtagaggaa cagacgcatc accacaccga agatagcgat tttgctcgcc gtcgccagga
     2161 aagtggaaac cggcgcaggc gcgccctggt atacgtctgg cgtccacagg tggaacggca
     2221 ccagagagag tttgaagcca aggccaacaa tcatcaggcc gaaacctgcc agcaacagcg
     2281 gctcgttgag cataccgtcg ccgaggtttt tacccagcgc gacaaacgac aggtcgccag
     2341 actgcgcata caccagcgcc ataccgaaca gcaggaaaga agacgctgcg gcagaaagga
     2401 tagtgtattt gatactggct tccagcgaac gtttctggcg gaaagcgtaa ccgaccaggc
     2461 caaacagcgg caaagagatc agttcgatac cgaggaacag agacgccaga tggttggcat
     2521 tcgccagcag gatcccgccc agcgcggcaa ttaacaccaa caggtagaac tcatccttgt
     2581 tgtcgttata gccttcaagc cacgggtagg cgaaagtaca ggtggcgagg ctcgccaaca
     2641 ataccagccc ggtgtaaagc atggcgaaac catcaacgcg catcagcggc gtaacgtcca
     2701 tagcgcccgc ctggccaaca aaccagagcg aaaccagcgc cgcgttaagc ccaataaccg
     2761 agagcgtagc gttgaggaaa tgattgcgtc gccacgcaat ggagagcatc acaaccacca
     2821 ccgtcaagcc gacgatcagc aacggtagca gtgcgatcag gttttgtgga gttattgtca
     2881 tggcgattta cggccttgta gtagtaacgg aattaacaaa ccactgctgg atattgccaa
     2941 tcgcggagtg cgaggtatcc agaatcggct gcggatagaa gcccagcagt accagcagca
     3001 ccaccagcaa caagatcata aacagctcac gcagcgacat ccctggcagt tcctggctgg
     3061 caatctggct tttcgcttta ccgaagtaag cgcgatgcaa catcgccagc gaataaacag
     3121 atgcaaagac cagcccaaag gtagagataa cggtaatcac cgggacaacc tggaagctgc
     3181 cgaacagaat cataaattcg ccgacgaagt taccggtgcc aggcatccca agcgttgcca
     3241 ccgcaaagaa cagcgacagt gctggcagcc atttcatctt gctccacaga ccgcccatca
     3301 tgcgcatgtc gcgggtatgg atacgttcat aaagctgacc acaaagaata aacagacccg
     3361 ccgccgacaa accgtgcgca atcatctgga ttaccgcgcc ctggtaggcc aactggctgc
     3421 cggtgtagat agcaatcagc acgaagccca tgtgggaaac cgaggtgtag gcgatcagac
     3481 gtttgatatc ggtctgggcg aaggccatcc acgcaccgta gaagatgccg ataacaccca
     3541 gccacatagc aattggcgcg aactctgccg acgcgttcgg gaacagcggc agggagaaac
     3601 gcagcaaacc gtaagcggca gttttcagca agatccccgc gaggtcaacg gaaccggcgg
     3661 tcggagcctg ggagtgcgca tccggcagcc agccatgcag cggaaccacc ggcattttga
     3721 ctgcgaaggc gatgaagaag cccagcatta acaggtattc cacaccactg gacattggcg
     3781 tattcagcag ctcttcatag ttgaaggtcc agacgccggt cgcattgtag tgaacaaaaa
     3841 ccagcgccag gatggcaatc aacatcacca ggccactcgc ctgggtgtaa atgaagaact
     3901 tggttgccgc cgtgatacgc gttttaccgt cagaggcttt atgcccccac agtgcgatca
     3961 ggaagtacat cggcaccagc atcatttccc agaagaagaa gaacaggaac atgtcgatgg
     4021 caaggaacac gccgataacg ccgcccagga tccacatcag gttgaggtgg aagaagccct
     4081 gatatttttc gatctctttc cacgaacata gtaccgccag cacaccgagc agaccggtca
     4141 gcacgaccat cagcagcgac agaccgtcaa tggcgagatg aatagagata ccaaaacgcg
     4201 ggatccacgg catgtcgaat tcagactgcc actgcggaat tccggcggat tgcgtcagtg
     4261 aataaccgcc ctgcaaccac agttgcagcg acagcgccag cgtcaatccc atggtgatca
     4321 gcgcgatcca gcgcggcacc ttgacgccaa agcgttcggt ctgccagcac aggaagccgc
     4381 caataaaggg aattaatatt agccagggta gtaacatggc gatctttatt ccttgtaaaa
     4441 gtcccgtcag gaccggattt tcaacgaatt ctcacgacag aaaattctct tcggactggg
     4501 ggtagtgtcg gatgcgccgc gaaggcagct tatccgacct acgttcttat atgcccaggg
     4561 gcaatcccac aatccttaac tcaacgcagt accatcaaca gtgccagcac cacgaccgca
     4621 ccgatgctca tggatgccac ataccagcgc agataaccgt tctcgcttaa cagcagacct
     4681 ttacctgcaa agcgggaaag aaccgccggg atgttcatca ttgagttcag cggatcgcgt
     4741 ttcagcaacc aggcaatacc caggaacggc ttgacgaaca ctttgtcgta cagccagtca
     4801 aagccccagg cgttgtacca ccaggtactc agcaggcggc ccggcgcact gttggcgatg
     4861 gaggtcacca gagtacgttt acccagccac agccaggctg ccagcagaat gccgaccacc
     4921 gcgaccacgc cagaggtaat ttccagggtc aacatgctgc cgtgcgccag ttccgtcgtt
     4981 tgcggaagca cgccctgcag cggcggtaca atcagtgcgc caacgaaggt ggaaaggatc
     5041 agcagcacaa tcagcggcag gctgtgagtt acccctttca cggcgtgagc gtgaatttgt
     5101 tcttttccgt ggaagacgat gaaaatcata cggaaggtgt agagcgaggt cataaacgca
     5161 ccgaccagac ctgccaccat cagattgata tgaccattcg ccatcgcacc cgcgaggatc
     5221 tcatccttac tgaagaagcc cgcagtgacc agcggtagtg ccgacagtgc tgcgccgccc
     5281 accaggaagc agagataaac cagcggaata gatttacgca gaccgcccat cttgaagatg
     5341 ttctgttcgt gatggcaggc cagaatgacg gaaccggatg ccaggaacag cagcgcttta
     5401 aagaacgcgt gagtcatcaa gtggaaaatc gccgcatccc atgcctgcac gccaagcgcg
     5461 aggaacatgt agccaatctg gctcatggta gagtaagcga gaacacgttt gatgtcggtc
     5521 tgtaccagcg cggcaaaacc ggccagcagc agcgtaaccg ccccgacaat acccaccaga
     5581 tgcagaactt ccggcgtcat caggaacagg ccgtgggtac gggcgatcag gtagacaccc
     5641 gcggttacca tcgttgcggc gtggatcagc gcggagacag gcgtcgggcc cgccatcgcg
     5701 tcagcaagcc atgtctgcaa cggcaactgc gcagatttac cgaccgcacc gcccagcagc
     5761 atcagcgtcg cccacatcag catgttattg ccgtcagcaa agtgcgctgg tgccagttcc
     5821 accatttcgc ggaagttcag ggtgcccagt tcgttgtaaa gaatgaacag tgcgaaagcg
     5881 aggaaaacgt cacccacacg ggtcacgacg aacgctttca ttgccgctgc gccattcttc
     5941 ggatcggtgt aatagaaacc gatcagcaga taggagcaca ggcccacgcc ttcccagccg
     6001 aggtacatca gcagcaggtt atcggcgaga accagaacta ccatgctggc gatgaacagg
     6061 ttggtgtagg cgaagaagcg agagtagccc tcttcaccgc gcatgtacca ggaggcgtac
     6121 atgtgaataa ggaaacccac accagtgacc accgagagca tggtcagcga caggccgtcc
     6181 agcaccaggt taaaaccgat gttaaagtcg cctaccgaca tccacgtcca cagcggctgg
     6241 ctgtatgcct gctcgccgtt agcgaagaaa tcaacgccga taaaggcggt taccagcgcc
     6301 gccaggccca cagagccaac gccgacgatc gccgaaacgt tttcagacca gcgcccacgg
     6361 gagaacgcca gcaggacgaa gccaatcaat ggcaaaataa tggttaaggc aagcatgttc
     6421 atccgcgcat ctcacttact gaatcgatgt tcaggttctg gcgacgacgg tgaagttgca
     6481 gcagcagcgc aaggccgata ctcgcttctg ccgccgcgag gctgatggcg agaatgtaca
     6541 tcacctgacc gtcggtctga ccccagtagc ttccggcgac cacaaaggcc agcgcggagg
     6601 cgttaatcat gatttccaga ccaatcagca taaacagcag attgcgacgg ataaccagac
     6661 cggttaagcc aagaacgaat aagattgccg cgaggatcag tccatgttgt aaggggatca
     6721 tgcgtgctcc tccgtttttc ttttcgcgct gtcgtcttta cgattgctca gcacttcacc
     6781 cgcacgctct tcacgaccga cgtggaaggc cacaaccaga cccgcgagca gcagcataga
     6841 agccagttcc accgccagta cgtaaggccc gaacagcgta atacccactg ctttagcact
     6901 gattggcgta ccgtcgatac cctgatcatt aacaccaagg atggcgtaaa caatcaccac
     6961 cagcatgatg gccgacaaaa ttgccggacc aatccacacc tgcggtttca gccactggcg
     7021 ttcctgttcg atttctgaac cgcccaggtt gagcatcatc accacgaaca cgaacagcac
     7081 cataatggca cccgcgtaga cgataatttc cagcgcaccg gcgaagtaag cgcccagtga
     7141 gaagaacacc ccggaaatcg ccagcagcga aataatcagg tacagcagtg cgtgtaccgg
     7201 attggtatgg gtgatcactc gcaaggtcgc aagtatggct atcaggccac agatataaaa
     7261 agcgaactcc attgcccctc tccttacggt aacaggctct tgacgtcgat aggcttggct
     7321 tcgttctctg cttcgccctt atctttgccg tcgattgcca tacctgccat ccggtagaag
     7381 ttatattccg ggtatttgcc cggaccggag atcagcagat cctctttctc gtaaaccaga
     7441 tcctggcgct tgtattcccc catttcgaaa tccggggtta actgaatcgc cgtggtcgga
     7501 caggcttctt cgcacagacc acagaaaatg cagcgtgaga agttgatgcg gaaaaattcc
     7561 gggtaccagc gaccgtcttt ggtttctgct ttttgcagcg agatacagcc gaccgggcag
     7621 gctaccgcgc agaggttaca ggctacgcaa cgctcttcgc cgtccgggtc gcgggtcaga
     7681 acgatacgac cacgataacg gggcggcaga tagaccggct cttccgggta cattcgcgtt
     7741 tcgcgtttgg cgaacgcgtg caggccgatc atccagatac tacgaacctg ggtgccgaaa
     7801 cctactaaca attcttttaa ggtcatggtc ttattgcccc ttattgcgcc tgccagagaa
     7861 tgacagccgc cgttaccagc aagttgatca gcgtcagcgg caggcagatt ttccagccga
     7921 aggacattac ctggtcataa cgcggacgcg gtaacgacgc acgaatcaaa atgaacatca
     7981 tcataaagaa cgcggttttc agcgcgaacc agatgaatgg cggtaacaac gggccttgcc
     8041 agccaccgaa gaacagcgtc accatcaatg cagagatggt cacaatcccg atgtactcac
     8101 ccacgaagaa cagaccgaac ttcataccgg aatattcaat gtggtaacca tccgccagtt
     8161 cctgctcggc ttccggctgg tcaaacgggt gacggtgaca taccgccacg cccgcgatgg
     8221 caaaggtaat aaaaccaaag aattgcggga taacgttcca cacatgcgcc tggctgttga
     8281 cgatgtcggt catgttgaat gaaccggcct gcgccaccac gcccatcagg gaaagcccga
     8341 ggaacacttc gtagctcagg gtctgcgcag aagcacgcat cgcacccagc aacgagtatt
     8401 tgttgttact tgaccagccc gcaaacagca ccgcgtaaac cgccagacct gccatcatca
     8461 ggaagaacaa aatcccgatg ttcaggtcgg caaccaccca acccggactg actggcacaa
     8521 tcgcaaaggc cagcagcagc gaggtaaagg caatcatcgg tgccagggta aagatgacgc
     8581 gatccgagaa tttcgggatc cagtcttctt taaagaacat tttgatcatg tccgcaacca
     8641 gctggagcga accgccccag ccaacacggt taggtccgta acggttctgg aacagaccca
     8701 gcaggcgacg ttcgccaaag ctcatgaatg ccccgcaggt gacaaccacc agcaggatca
     8761 ccaccgcttt gaggatggtc agcaggatct caatcagttc cggtgatatc caactcattg
     8821 ttgtgcctcc ttgagatcct caagatgcgc gccagccagc accggagcaa taccggacat
     8881 acccatcggc aagcccacct gccctgccgt cagcccttca gcgatttcaa ccggcagcgt
     8941 gaccgtgttg ccatcgtaac taaaggagac gtgtgtacct gcgttcacac ctaacttcgc
     9001 ggcatccgct gggttgagtt tgatgtacgg ctgcggcata cggctctgga agaccggagc
     9061 acgctgtgac aattcatcgc tgccaaacag gtggtaatac ggcgcgatac gccatttccc
     9121 gtcctgcggc tggaagcgtg ccggtacgct ggtgaagtaa tccagaccat tttcgctggt
     9181 ttcaaacaga cgcacgcccg gatcgccaaa gcgcagtttg ccgcccactt cgtcctggaa
     9241 tttgttccac gcctgcgggg agttccagcc cggcgcccag gcaaacggca cttgcgaacg
     9301 gtgcgcagtc ggctggttgt taccttccat cgagaaggtg aacatggtgt caatatcctg
     9361 cggctgacgc ggctcatgaa cgctgatatt ggcgcgcatg gcggtacgac cgctgtaacg
     9421 gtgcggttca cgggccagtt tctgcccacg aatacggaat gtcgcatccg gcgcagcatc
     9481 tttgatacct gccagttccg ggattttcgc cacaacagcg tcaatcacat ggtcgagctg
     9541 cgtccagtcc acttcacggc tcagcagggt gctgtgcagc gagtgtaacc agcgccagct
     9601 ttccagcatg acagttttgc tgtcgtaata agcaggatcg taaacctgga agaaacgttg
     9661 ggcgcggcct tcgttgttga tcaccgtacc gtcgctttca gcaaagctgg cagcagaaag
     9721 taccagatgg gcgttttcca taatcgctgt gcgttgatga tcaaccacca tcaccagcgg
     9781 cgctttagcc agcgcagcat tcacgcgggt agcggaagcg tgacgatgca gatcgttttc
     9841 cagcaccacc accgcgtcgg cgcgtccggt ttccagttcg gtcaacgctt cttcaagcga
     9901 accgccaccc ataatgccca gccccatgct gttgacggaa cgggcaatca tggtgatacc
     9961 aacgtcagcg ccgcgacctt tcagggcttt cgcgacgtta gccgcagcct gaatcacctc
    10021 tatgctaccg gcgttcgtcc cggagataat caacggtttc ttcgcacctg ccagtgcctg
    10081 tacgatgacg tcgattttgc tttgcagctc aggttcgata ccgtcaaccg ctggcgcaga
    10141 gttatccagc gcatgggcga tggcaaaacc taaacgcgcc tgatcttcaa ccggtgcgcg
    10201 gtaagtccac gccgcgatat catccagacg ggtgtcatca acgttggtaa caaacagcgg
    10261 atgcttcgca cgttgaccga tgttgaggat tgccgcaatc tgccagtcag ccactttctg
    10321 tgctgccgcc atttcgcgcg ctttaccttt cacagcctga cgcactgcca gcgcgacgcg
    10381 cgcgccggtc tgggtaacgt cttcgcccag caccagtacc gcatcgtaag attcgatttc
    10441 gcgcagagcc ggagtataaa tgccgccttc acgcagcact ttcagcgcca gttgcagacg
    10501 ttcctgctca ccgtgagcga taccggtgta gaagttttct tcgcccacca gttcacgcag
    10561 cgcaaagttg ctttccacgc tggcacgcgg agaaccaata ccgatcactt tcttcgactg
    10621 acgcagaata tctgccgcgc cctgcattgc ctgttcggcg ttgagggtaa tgaaatcatc
    10681 gccacgacgc tgtactggtt gacgcggacg atctttcagg ttgacgtaac cgtaaccgaa
    10741 acgaccacgg tcgcagagga agtagtggtt taccgtaccg ttgtaacggt tttcgatacg
    10801 acgcagttcg ccgtaacgtt caccggggct gatgttacag ccgatggaac attgctggca
    10861 gatgctcggc gcaaactgca tatcccattt acggttgtaa cgctcggagt gcgttttgtc
    10921 ggtaaatacg ccggtcgggc aaatttcgac caggttaccg gaaaattcgc tttccagcgt
    10981 gccgtcttcc gggcgaccga agtagacgtt gtcgtgcgca ccgtaaacgc ccagatctgt
    11041 accgtcagcg taatctttgt agtaacgcac acagcggtag caggcgatgc agcggttcat
    11101 ttcgtgagag atgaatggcc ccaaatcctg attacggtgg gtacgtttgg tgaaacggta
    11161 gcgacggaag ctgtgtccgg tcatcacagt catatcctga agatggcagt taccgccctc
    11221 ttcacatacc ggacagtcgt gcgggtggtt ggtcatcaac cactcgacca cgctttcacg
    11281 gaactgtttc gcttcttcgt cgtcaatgga aataaaggtg ccatcggaag ccggtgtcat
    11341 acaggacatc accaggcgac cacgcgtgtc ttccgcgttt tggtattgct tcaccgcaca
    11401 ctggcggcaa gcaccgacgc ttcccagcgc cggatgccag caaaagtaag gaatatcaag
    11461 gcccagagac agacaagctt ccagcaggtt gtccgctccg ttgacctcgt attctttgcc
    11521 gtctacatga attgtagcca ttagcatgct tccagttttc tcggtcaggg accgagcgtt
    11581 aatcgaaatt cggttaccag cgctctttca gcaggttcgg ctgaatccca ttaatcaaat
    11641 gggtattgct gaacggctgt ttgattcccg cctcaaattc ttcgcggaaa tatttgatgg
    11701 cgctatgtaa cggctccact gcaccaggtg cgtgggcaca gaaagtttta cccgggccta
    11761 agaatcgaca cagttgctca agtgtttcga tatcgcccgg ctgaccttca ccacgctcca
    11821 gcgcacgcag aattttcacg ctccacggca gaccgtcgcg gcacggcgta caccagccgc
    11881 aggactcacg ggcgaaaaac tcttccaggt tacgcaccag cgacaccatg ttgatctcat
    11941 ggtcaaccgc catcgccagc gccgtaccca gacggcttcc cgctttaccg atactttcga
    12001 attccatcgg caggtcaagg tgcgcttcgg tcaggaagtc ggtgcccgcg ccgcctggct
    12061 gccaggcttt gaatttcaga ccgtcacgca taccaccggc gtaatcttcg aggatctcgc
    12121 gcgcggtagt accaaacggc agttcccaca gtcccggatt tttcacccga ccggagaagc
    12181 ccatcagctt ggtgccagca tctttacttt tcgagatgtt ctgataccac tccacgccgt
    12241 tagcgaggat cgccggaacg ttacacaggg tttcgacgtt gttgacacag gtcggtttgc
    12301 cccatacgcc ggaggttgcc gggaaggggg gtttcgagcg tgggttagca cgacgccctt
    12361 ccagggagtt gattaacgct gtttcttccc cgcagatgta gcgccctgcc ccggtatgga
    12421 cgaacagttc gaagtcgaaa cctgttccca taatgttttt gccaagcaag cccgcttcag
    12481 tggcttcggc aatggcgcgg cgcagattta ctgccgcttc gatatattcg ccacgcagga
    12541 agatgtagcc acggtaagct ttcagcgcaa acgcggagat gagcatacct tccaccagca
    12601 ggtgcggcag ttgctccatc agcaggcggt ctttataggt gcctggctcc atttcatcgg
    12661 cgttacacag caggtaacgg atgttcatgg attcgtcttt cggcatcagg ctccatttca
    12721 ggccggtaga aaagcccgcg ccaccgcgcc ctttcagacc agcgtctttt acctgattaa
    12781 cgatttcgtc cggagacagc ccggtcagcg ccttacgcgc gccttcgtaa ccgtttttgc
    12841 tgcgatattc gtccagccac actggctgtt tgtcatcgcg cagacgccag gtcagcggat
    12901 gcgtttcggg agtacggata atgtttttca tttataccgc tccagcagtt cagggatcgc
    12961 ttccggggtc agatgcgcgt gagtatcctc atcgatcatc atgtttggcc ctttatcaca
    13021 gttccccagg cagcaagttg gcagcagcgt aaagcgaccg tcaaatgtcg tttgccctgg
    13081 tttgatgttt agctttttct cgagcgccgc ctgaataccc tggtaaccgt tgatatgaca
    13141 gaccacgctg tcgcaataac ggatcacatg gcggccaacc ggctggcgga agatctggct
    13201 gtagaacgtt gccacacctt cgacgtcgct tgccggaata cccagcacat cggcgatcgc
    13261 gtggatcgca ccatccggca cccagccacg ctgcttctga acgattttca gcgcttcaat
    13321 ggacgccgca cgcgggtctt cgtagtggtg catctcgtgc tcaatcgctt cacgctctgc
    13381 cgcactcagc tcaaaagcct cggtttgtgg ttgttgattc tcgtgcataa ttagcggtcc
    13441 acatctgaca taacaaaatc gatactgccc agataaacaa tcaggtcaga caccaggctg
    13501 ccgcggatcg ccgccggaat ttgctgcaaa tgcgcataac tcggcgtgcg gatacgagtg
    13561 cgataactca tggtgctacc gtcgctggtc aggtagtaac tgttgatccc cttggtcgcc
    13621 tcaatcatct ggaaagattc attggcaggc atcaccggcc cccacgacac ttgcaggaag
    13681 tgggtgatca gggtttcgat atgttgcagc gtgcgctctt tcggcggcgg cgtggtcagc
    13741 gggtgatccg ctttgaacgg gccttccggc atgttgttga ggcactgctc aagaatgcgc
    13801 agactctggc gcagctcttc cactttcagc attacgcggg tgtagcagtc agaaacacca
    13861 ccaccaaccg ggatttcaaa gtcgaagttt tcatagccag aataaggacg cgccttacgc
    13921 acgtcgaagt cgatcccggt agcacgcagg cccgcgccag tggtgcccca ctccagcgcc
    13981 tctttcgcgc cataggcggc aacgccctgg gaacgacctt tcagaatggt attttgcagc
    14041 gccgctttct cgtaagacgc cagacgtttc ggcatccagt cgaggaactc acgcagcagg
    14101 cgatcccagc cgcgcggcag gtcgtgcgct acgccgccaa tacggaacca cgccgggtgc
    14161 atacggaaac ccgtgatcgc ttccaccaga tcgtaaattt tctgacgatc ggtaaaggcg
    14221 aagaacacgg gcgtcattgc gccgacgtcc tgaataaagg tcgagatgta cagcaggtgg
    14281 ctgttgatac ggaacagttc ggagagcata acgcgaatga cgttaacgcg atccggcacg
    14341 gtgatcccgg ccagtttctc taccgccagc acgtaaggca tttcgttaac gcagccgccg
    14401 aggtattcaa tacggtcggt atacggaatg tagctgtgcc aggactggcg ctcgcccatt
    14461 ttctccgcac cacggtggtg gtagccgatg tctggtacgc agtcgacaat ctcttcgccg
    14521 tcgagttgta aaacgatacg gaaagccccg tgcgccgacg ggtggttcgg accgaggttg
    14581 aggaacatga agtcctcgtt ttcggtgccg cgcttcatcc cccactcttc cggtttgaag
    14641 gtcagggctt ccatctccag atcctgtttg gctttggtca gctcaaacgg cgagaattcg
    14701 gtagcgcgcg ccgggtaatc tttacgcagc gggtgacctt tccaggtttg cggcatcatg
    14761 atgcggcgca ggttcgggtg accgtcgaaa gtaatgccaa acagatccca ggtttcacgc
    14821 tcataccagt tagcgttcgg gaacagtttg gtgaaggtcg gtacgtgcag gtcgttttct
    14881 gccagcgcca ccttcagcat gatgtcgcgg ttacgatcga tagaaatcag atggtagaaa
    14941 acggaaaaat ccgcggcagg taacccttcg cggtgtgtgc gcagacgttc gtccatgccg
    15001 tgtaagtcaa acagcatgac gtaaggtttc ggcagtttct ttaagaaatc gccaacttcc
    15061 agtaattgtt cacgcttgat ccacacaacg ggaaccccgg tgcgagtcgc ctgaacagta
    15121 aaggcatccg gcccaaaacg gttgcgcagt tcgccaatca ccggatcatc aagatgatcg
    15181 cgggtctgcc aggcgggttc ttgcgcggtt aagtcggtca tattgttcac cattgcaaat
    15241 ggtccgtggt gactgtcggc gcagtaagct tcgcgaaata gatttaggaa taagcgaaga
    15301 aaaatccctt tgccgacagg cgcaaattaa atctcgtcag gtgtacgcag gttggttacg
    15361 gcaatgcgtt caccgcgctt gcgttcgcgc tctgattgca tattggcgcg ataaacgccc
    15421 tgatcgccaa ccacccagga gagcggacga cgttctttgc cgatagattc ctgcaacagc
    15481 atcagtgcct gcatgtacgc ttcaggacgc ggcgggcagc ccgggatata cacatcaacc
    15541 gggatgaatt tatcgacgcc ctgcacaacg gaataaatat cgtacatacc accagagttg
    15601 gcacaggcac ccattgagat aacccatttt ggttccagca tctggtcata cagacgctga
    15661 ataaccggtg ccattttggt aaagcaggtt cctgcaacca ccatcaggtc agcctgacgc
    15721 ggcgaagcac gcaatacttc tgcgccaaaa cgcgccacgt catgcaccgc ggtaaacgaa
    15781 gtcaccatct caacgtaaca gcaggaaaga ccgaagttat acggccaaat tgagttttta
    15841 cgaccccagt taaccatgtc attgagcttg cccataaaca cgtttttgtt aacttcttgc
    15901 tccagagggt cggttacgat ctcctgcttt tgcagggggt aacggtcgtt ctcaccgttg
    15961 ggatctatgc gggtgagcgt ataatccatc ttaatgcctc gcggttagcg ttgacgatta
    16021 gcgatactgt tcgtttccgg gttcatacgc tcgcggcgtg aacgcgcggg cgtccagtcc
    16081 agcgcgccaa tacgcaccag ataaaccaga cctgccagta acacaaaaat aaaaattgca
    16141 gcttccacaa agcctaccca gccgctttcg cggatagagg ttgaccatgc gaacagatac
    16201 agcgcttcaa cgtcgaagat aacgaagaac atggccacca gataaaactt ggcggacagg
    16261 cgtaagcggg cggagccgac cgagtcgata ccggattcaa acggcacgtt tttcgacctc
    16321 gcgcgtgcgc gaccgcctaa aaaccaaccg cctaccagca tcaggcaaca caggccaatg
    16381 gcaacgataa gaaagatagc gaatgcccag tgatgagcga tgacttcagt ggatgttgac
    16441 atactcattg cttactcatc aaaagtagcg ccagattcac tgctcttcac ggcagatgga
    16501 cgccacatcg attcatgggg aggaataaaa aaaaccttac aatcactgta gaaattcttt
    16561 tatacagcta attgatgtgg tcttttactc ctttctataa ccttttgtca actttaacaa
    16621 aagtttcttc acattagttt acataatatc aacaccatta gcatttaatg ccctttcacc
    16681 ccagatcctt gacgactcca ggataattag atgttgttga atcgtgtccg ttgtgaagca
    16741 atggaaaaaa tacgggtcta ttttgacagg aatttgtgtc gattcctccc cccaaaagag
    16801 agtattttct tgatctgtga cacgcttttg tcattccata acaaaaacgc agcaacaaat
    16861 ttacgtattt tttaacatca ttgtagcagg tgattttttt caggcgatta tttgtgcgtt
    16921 cgggacgtga atctctggtg attgaaaaat gaacagtttt gtacgttctg cactatgcga
    16981 ggaaggattt ttactaaaaa aaagccgctg gggtttaaaa cacccccagc ggctcgtttt
    17041 ttacactatt gtctcaggaa ttatctatcg tccgtcgatt actcgatatc cctttcaatc
    17101 aacaatgaat catccccttc cggagcagac attgggctgt attgccacgg attatggtag
    17161 ctctccatcg cctgatagat cacctgggcc agctcattat tactggacgg gtcatagcac
    17221 agcaaatatt cggtatcagg tagcggcggt aagccatcta ctccactcag cacacgcagg
    17281 tccgggctca tcatctcaac cggccttgcc gtcacgccaa gaccggcttt cactgccgca
    17341 cgaacggccg gaagcgtcga ggcgacataa gccagtcgcc atggaatatc tgctttatta
    17401 agcgtcgcca gcaccatatc gcgaaacgga cttggatcat ccagcaatac aagagggatc
    17461 ggctcgcctt tttgcaatac gtattccgct gcgcagtacc agtgtgttgg cgaggttcgc
    17521 aggttcaatg ccttaaacgc tgaaggacga tgggtggtta ccatcaaatc cacttcctgg
    17581 gattccagca tttctgccat ataggcatta cgtttaacgc ggacatccag cgctaatttc
    17641 ggataaaccg aactcacgcg atttaacagg aaaggtaaga tcgtatcggc agattcatct
    17701 gaagcaccga tagttaacac gccctgaaga ttactgaaca ttaatgatga gcaggcctca
    17761 tcattaaaac gcaggatttt cctggcgtaa ccaagaagtt gaatgccatg ttcagttaac
    17821 agtttgttgc gaccgtgacg agcgaacagt tctttcccaa cgagttgttc cagacgctgc
    17881 atttgctgac ttacggcgga ctgagtacga cacacagcgg cagctgcggc agcaaaagtg
    17941 ttcagatcgg caacagcaac aaatgttctc agcagatcga ggtcgaggtt aattatcgga
    18001 cgatttgcac ttatcatata ttatcactta ctggcggctc atactgagct ggttaatgct
    18061 gtgcacacac aaacaagcaa ttccatttgt aatgtgcctc cctggcagtt tcatccggaa
    18121 cccggacgaa agtaaaaatg catatgagtt gcactaaaaa agcgactcac attgttccgt
    18181 tataatgcct gaagtagatc acagaatata tcttcaggga tcgcatatct attaagttac
    18241 tcactctttt ctatttatga catgcgcgtg tttgtataaa tgtaaatgtg agtccttgtt
    18301 ccactctcgt gcagcatcgc tggtcatacg cgaacacgta caaacagcaa tggtgaggcg
    18361 gcatcaagag cagggatccg tcatttatgc gagcatttta ccccaaaaac cttttattta
    18421 taagggtcat tgcgaa
//
LOCUS       DAZGAN020000094        18340 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGAN020000094 DAZGAN020000000
VERSION     DAZGAN020000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.094"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(300..1892)
                     /gene="lsrK"
                     /locus_tag="ACHKYO_RS17995"
                     /old_locus_tag="pgaptmp_003599"
     CDS             complement(300..1892)
                     /gene="lsrK"
                     /locus_tag="ACHKYO_RS17995"
                     /old_locus_tag="pgaptmp_003599"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HGU4430375.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1971..2924)
                     /gene="lsrR"
                     /locus_tag="ACHKYO_RS18000"
                     /old_locus_tag="pgaptmp_003600"
     CDS             complement(1971..2924)
                     /gene="lsrR"
                     /locus_tag="ACHKYO_RS18000"
                     /old_locus_tag="pgaptmp_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HGU4430376.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3173..4708
                     /gene="lsrA"
                     /locus_tag="ACHKYO_RS18005"
                     /old_locus_tag="pgaptmp_003601"
     CDS             3173..4708
                     /gene="lsrA"
                     /locus_tag="ACHKYO_RS18005"
                     /old_locus_tag="pgaptmp_003601"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HGU4430377.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVEAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4702..5730
                     /gene="lsrC"
                     /locus_tag="ACHKYO_RS18010"
                     /old_locus_tag="pgaptmp_003602"
     CDS             4702..5730
                     /gene="lsrC"
                     /locus_tag="ACHKYO_RS18010"
                     /old_locus_tag="pgaptmp_003602"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HGU4430378.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKNREAA"
     gene            5730..6722
                     /gene="lsrD"
                     /locus_tag="ACHKYO_RS18015"
                     /old_locus_tag="pgaptmp_003603"
     CDS             5730..6722
                     /gene="lsrD"
                     /locus_tag="ACHKYO_RS18015"
                     /old_locus_tag="pgaptmp_003603"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HGU4430379.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVLLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6734..7756
                     /gene="lsrB"
                     /locus_tag="ACHKYO_RS18020"
                     /old_locus_tag="pgaptmp_003604"
     CDS             6734..7756
                     /gene="lsrB"
                     /locus_tag="ACHKYO_RS18020"
                     /old_locus_tag="pgaptmp_003604"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HGU4430380.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVTPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7783..8658
                     /gene="lsrF"
                     /locus_tag="ACHKYO_RS18025"
                     /old_locus_tag="pgaptmp_003605"
     CDS             7783..8658
                     /gene="lsrF"
                     /locus_tag="ACHKYO_RS18025"
                     /old_locus_tag="pgaptmp_003605"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HGU4430381.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEICWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8682..8972
                     /gene="lsrG"
                     /locus_tag="ACHKYO_RS18030"
                     /old_locus_tag="pgaptmp_003606"
     CDS             8682..8972
                     /gene="lsrG"
                     /locus_tag="ACHKYO_RS18030"
                     /old_locus_tag="pgaptmp_003606"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HGU4430382.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            9029..9787
                     /gene="tam"
                     /locus_tag="ACHKYO_RS18035"
                     /old_locus_tag="pgaptmp_003607"
     CDS             9029..9787
                     /gene="tam"
                     /locus_tag="ACHKYO_RS18035"
                     /old_locus_tag="pgaptmp_003607"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HGU4430383.1"
                     /translation="MSDWNPSLYVHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9791..10705)
                     /locus_tag="ACHKYO_RS18040"
                     /old_locus_tag="pgaptmp_003608"
     CDS             complement(9791..10705)
                     /locus_tag="ACHKYO_RS18040"
                     /old_locus_tag="pgaptmp_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HGU4430384.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10912..12363)
                     /gene="uxaB"
                     /locus_tag="ACHKYO_RS18045"
                     /old_locus_tag="pgaptmp_003609"
     CDS             complement(10912..12363)
                     /gene="uxaB"
                     /locus_tag="ACHKYO_RS18045"
                     /old_locus_tag="pgaptmp_003609"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HGU4430385.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGVHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAQGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12590..14008)
                     /locus_tag="ACHKYO_RS18050"
                     /old_locus_tag="pgaptmp_003610"
     CDS             complement(12590..14008)
                     /locus_tag="ACHKYO_RS18050"
                     /old_locus_tag="pgaptmp_003610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU4430386.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14147..14506)
                     /locus_tag="ACHKYO_RS18055"
                     /old_locus_tag="pgaptmp_003611"
     CDS             complement(14147..14506)
                     /locus_tag="ACHKYO_RS18055"
                     /old_locus_tag="pgaptmp_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HGU4430387.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14506..15432)
                     /gene="glsB"
                     /locus_tag="ACHKYO_RS18060"
                     /old_locus_tag="pgaptmp_003612"
     CDS             complement(14506..15432)
                     /gene="glsB"
                     /locus_tag="ACHKYO_RS18060"
                     /old_locus_tag="pgaptmp_003612"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HGU4430388.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15496..16884)
                     /gene="sad"
                     /locus_tag="ACHKYO_RS18065"
                     /old_locus_tag="pgaptmp_003613"
     CDS             complement(15496..16884)
                     /gene="sad"
                     /locus_tag="ACHKYO_RS18065"
                     /old_locus_tag="pgaptmp_003613"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HGU4430389.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16985..17866
                     /locus_tag="ACHKYO_RS18070"
                     /old_locus_tag="pgaptmp_003614"
     CDS             16985..17866
                     /locus_tag="ACHKYO_RS18070"
                     /old_locus_tag="pgaptmp_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4430390.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17944..>18340
                     /gene="yneK"
                     /locus_tag="ACHKYO_RS18075"
                     /old_locus_tag="pgaptmp_003615"
     CDS             17944..>18340
                     /gene="yneK"
                     /locus_tag="ACHKYO_RS18075"
                     /old_locus_tag="pgaptmp_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HGU4430391.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNP"
BASE COUNT         4640 a         4660 c         4537 g         4503 t
ORIGIN      
        1 ctttcaatag gaaaattatc atcatcatat ttaacaaaga atagcactaa ttgctaaaaa
       61 tcgaagttta ttaaacccct tttgcatcca ttagtgtctt taggaatatt cgctataaaa
      121 taagggttgt gcttaatgct ttaagaaaaa tagcaatttt cccttgaata tagtactggt
      181 gatggaacga tgaatctgca agctggcttt taacaagcca gctctaaaag aagggaaata
      241 agaataacta tactcaaaaa ctaacagcca cggtcatcat gatgtggctg tcaatgaaac
      301 tataacccag gcgctttcca taacgacgtc gtcagtccat gatcaaccag ccccagctga
      361 tcctgataaa ctgcctgcca cttatcgcgt gaatcctgat aaagttcatg cttttccggg
      421 tctggtgtgt gcgtccgttc ccagcgaacc aggcgttctc cggtttctgc cattgatgaa
      481 aaaattccgg caccgacgcc agctgcaatg gcacatccta atgcagtggc ttctttgacc
      541 accggaatat tgacgggtaa tcccgagaca tcagcgagaa tttgactcca taatttccct
      601 tttgaacctc cgcctgcaaa gactaacgat gaaggatgaa tattcgagaa atcagcaatt
      661 tgctgcaagt tacacgctga tacaatcgcc gcattttctt ccagcgcacg gaacaatgtc
      721 gctttgttac atttatccgg gtcaatggac aagttaataa aggaaggcgc agcgtgatac
      781 caggttttaa agcgcattct gtcggagaag atcggcatta cgccccacga cccaggcggc
      841 acccgactgg ccatctcttc cagcagcgta taggtgtcga tgcctaaacg ttcggcaatc
      901 agtttttctt cggcacagaa agcatcgcgg aaccagcgca tggtgagtcc ggtaaaaaag
      961 cttatagatt cagcttgtac catgccagga ataacatgag gattaacgcg cacgttcatt
     1021 tctgggtctg tcaccggcgc ggctaaattt acaacttgct gccagaatgt gccgccaaga
     1081 accgcggttt gtgccggacg cacaacgcct aacccaaggc aaccaagctg cacgtcgcct
     1141 cctccaacga ccaccggagt gcccgccttc agaccgcaga gttccgccgc ttgtgaactt
     1201 accacgccca gcaatgtgcc ggtttctttg acaggagaaa gaatatcggc acgtaggcca
     1261 gccatatcca gcaatgcagg tttccagtca cgggtggtta gatcaagaag tcccgtggtg
     1321 ccagcgttag agggatccac cgccagttcg ccgctgagca tataggccag ccagtcgctg
     1381 atcatggtga tggttgatgc ctgacggtaa atatcggaac gatggtgcgc cagccaaagt
     1441 aatctgggga tggcacttaa agccagtgtt tgtccggtcg cgcgataaac ttcgttttca
     1501 aaggtattgt tgtgcagttc tttaagttcg ctaacttcgc gtgccgctct ggcatccaca
     1561 ttggcgcagg cccagatcgg ggctccttca ttattatata aaacaatgcc ttcacgcatc
     1621 gaacatgccg aaacggcagc gatatactcc ggggctatgc cggcgttgtg cagcgcctgg
     1681 cgcatacact cacacgccag ttgccagttt ttgttgagat caaattccat agaaccagga
     1741 acgtccggta ctgccaggtg ccgccactcc gcctgtccca ctgctatttg attgccttcc
     1801 aggtcgaata tcacagcccg aatacttccg gtacctgcat ccagcgccat caggtaatac
     1861 tttgattctg aaggggtaaa gagtcgagcc attagattat cctcggctta tgcgttctgt
     1921 ctgttcctct atacgttctc catcattccc ggtaataagg tcatgcaaat ttaactacgt
     1981 aaaatcgccg ctgctgtgtc ctgatcggta accagtgcgt tgatataacc gcctttcatt
     2041 gcagcggcaa ttgcttcggc tttattttct ccccctgcca cgccaacccg gacgggtatg
     2101 gtcttcagcg agcttaaagg taagccaatc agttcgttat gtattttaat atccgtgaca
     2161 acgtcacctt ttgcatcaaa aaagtagcct aaaatgtcgc caaccgcccc ttttcgacca
     2221 atcattaact gttcgccctg gctgatataa ccggagcgaa tgatcgtcgc gtcgtcctgt
     2281 tgactgacgg ccccaatgcc gacaatcgcc acatcggctg cttgcgcggc taacagaaca
     2341 tctttgacgc aattttcatt ttttagcgta cgggcaatgt cagcggagga tgcccgcaac
     2401 ggagccggaa taatattcac actgcacgcc gcgttaagct gcccgatacc cgtcatataa
     2461 gaaccgacgc caccggagag cgtgaccagg cgaatttgct gtgacgaaat aaaaccactt
     2521 aagcgttgca gcgtattcat ggttgcctcg ccaaaaccaa tcgccagcat ctgttgtggt
     2581 tgaagtaaac tcatcaacat atgcgccgcg cctatcccca gtcgcccgcc gacatcagca
     2641 tccgcaagcc cagggatcac ccggacatgt tgcagcgaaa actgacgacg taattgagtt
     2701 tcatattcca gacagccttc aaagcgagaa ttaatctgta cgcgaataat gccggactga
     2761 tgccctttct ccagcaagcg cgacactttc aaacgggtca ggccgagacg atcgctgatc
     2821 tcgctctggg tcagcccgtc gtgatagtaa aaccacgcga tccgcgcgac ctgttcttct
     2881 tcacacattc cctgttctga aattgccgaa tcgttgattg tcataattca ttcttcactt
     2941 tgaacatatt taaatcttta atgcaattgt tcagttcttg cgcatttata tctgtgatgg
     3001 cagccacagt ttgactctac gagcatgaac aaacgcaacc gtgaaaatca aaatagcata
     3061 aattgtgatc tattcgtcgg aaatatgtgc aatgtccacc taaggttatg aacaaattaa
     3121 aagcagaaat acatttgttc aaaactcacc tgcaaaactg aacgggggaa atatgcaaac
     3181 gagtgatacc cgcgcgttac cgctactttg cgcccgctcg gtttataaac agtattcagg
     3241 ggtcaatgtc ctgaaaggca tcgattttac gttgcatcag ggggaggtcc acgccctgct
     3301 cggcggcaat ggtgccggta aatcgacgtt aatgaagatt attgccggta ttacccctgc
     3361 tgatagcggt acgctggaga ttgggggcaa caactacgcc agattaacgc cagttcatgc
     3421 tcatcagctg ggcatttatc tcgttcccca ggaaccgctg cttttcccaa gcctgtcgat
     3481 aaaagaaaac atcctgtttg ggatggcaaa aaaacagctc tccatgcaaa aaatgaagaa
     3541 cttgctggcg gcgctgggct gccagtttga tctgcatagt ctggcaggat cgctggatgt
     3601 cgccgatcgc caaatggtgg aaatcctccg cgggctgatg cgcgactcgc ggattctgat
     3661 cctcgatgaa cctaccgcct cgcttacccc tgcggaaacc gaacgcttgt ttagtcgctt
     3721 gcaagagctg cttgctactg gcgtgggtat tgtttttatc tcgcataagc tgccggaaat
     3781 tcgccagatt gccgatcgaa ttagcgtgat gcgcgacgga accatcgcct taagcggcaa
     3841 aaccagcgaa ctgtctaccg acgacattat tcaggccatc accccagcgg tacgggaaaa
     3901 atcgctctct gccagccaaa aattatggct ggagttacct ggtaaccgcc cacaacatgc
     3961 tgccggaacg ccggtgctga cactggaaaa tctgaccggc gaaggtttca ggaatgtcag
     4021 cctgacgctc aatgccggag aaattctggg cctggctggg ctggtggaag ccggacgcac
     4081 agaactggcc gagacgctct atggtctgcg tactttgcgt ggcggacgca ttatgctgaa
     4141 tggtaaagag atcaataaat tatccactgg agaacgttta ctgcgcggtc tggtttatct
     4201 gccggaagat cgccagtcat ccggactgaa tctcgatgct tcgctggcct ggaacgtctg
     4261 cgcccttact cataaccttc gtggattctg ggcgaaaacc gcgaaagata atgccaccct
     4321 ggaacgttat cgtcgggcgc tgaatattaa atttaaccaa ccggaacaag ctgcacggac
     4381 attatccggt ggcaaccagc aaaaaatcct cattgccaaa tgcctggaag cttcgccgca
     4441 agtattgatt gtcgatgagc cgacgcgcgg cgtggatgtc tcggcccgta atgatatcta
     4501 ccagctgttg cgcagcatcg ccgcacaaaa tgtggctgtg ctgcttatct cctccgacct
     4561 ggaagagatc gaactgatgg cagatcgtgt gtatgtgatg catcagggcg aaattgccca
     4621 ctctgcactg accgggcgcg atattaatgt cgagactatt atgcgcgttg ccttcggcga
     4681 tagtcagcgt caggaggcat catgctgaag tttattcaga acaaccgtga aatcacggca
     4741 ttgctggcgg tggtgctgct gtttgtatta cccggttttc tcgaccgcca gtatttaagt
     4801 gtgcaaacgc tgaccatggt ttatagcagc gcgcaaatcc tgatcctgct ggcaatgggc
     4861 gcgacgctgg taatgcttac gcgcaatatt gatgtttcag tgggttcgat taccggaatg
     4921 tgcgcggtgc tgttggggat gttactgaac gcaggatatt cactacctgt tgcctgtgtc
     4981 gcgactttac tgcttggttt gctcgcggga tttttcaacg gtgtcctggt cgcgtggcta
     5041 aagatccctg ccattgttgc cacccttggc acgttagggt tgtacagagg catcatgttg
     5101 ctgtggactg gcggtaaatg gattgaaggg ttacccgccg aactgaaaca gctctccgca
     5161 ccgctgctgt ttggcgtttc cgcgattggt tggttgacga taattctggt ggcatttatg
     5221 gcctggctgc tggcaaagac ggcgtttgga cgcagttttt atgccacggg cgataattta
     5281 cagggcgctc gtcaactggg tgttcgtacg gaagccattc gcattgtggc attttcgttg
     5341 aacggctgca tggcggcact ggcgggaatt gtgtttgctt cgcagattgg ttttatcccc
     5401 aaccagaccg gtaccgggct ggagatgaaa gcaattgcag cctgcgtgct gggcggcatt
     5461 agtttgctcg gcggttccgg tgcgatcatc ggtgcggtac tcggcgcatg gttcctgacg
     5521 cagatcgata gcgtactggt gctgttgcgc attccggcat ggtggaacga ttttatcgcg
     5581 ggcctcgtgc tgctggcggt gctggtgttt gatggacgcc tgcgttgtgc gctggaacgt
     5641 aatctacggc ggcaaaaata tgcccgcttt atgacgccac cgccatccgt taaacccgct
     5701 tcgtcaggta aaaatcggga ggccgcataa tgcgtattcg ctacggttgg gaactggctc
     5761 ttgccgcact gctcgttatt gagattgtcg catttggtgc aattaacccg cggatgttag
     5821 atctcaatat gttgctgttc agcaccagtg actttatctg cattggcatt gtcgccctac
     5881 cgctgacgat ggtgattgtc agtggcggga tcgatatttc gtttggttcg accatcggcc
     5941 tctgcgccat tgcattgggc gtactgtttc aaagtggtgt gccgatgccg ctggcgatac
     6001 tcctgacctt actgctcggc gcattgtgcg ggctgatcaa cgccggatta attatctata
     6061 ccaaagttaa cccgctggtg attacgcttg gcacgctgta tctgtttgcc ggaagcgctc
     6121 tgctgctttc cggtatggcc ggagcgacgg ggtacgaagg tattggtgga ttcccgatgg
     6181 cgtttacaga tttcgctaac ctggatgtgc tgggactccc cgttccgctg attatcttcc
     6241 tgatatgtct cctcgttttc tggctctggc tgcataaaac ccatgccgga cgtaatgtgt
     6301 ttttgattgg gcaaagcccg cgcgtggcgc tttatagcgc gattccagtt aaccgcacct
     6361 tatgtgcgct ctatgccatg acggggctgg cgtctgcggt cgccgctgtg ctgctggtgt
     6421 cgtattttgg ttcagcacgt tccgatctcg gtgcgtcgtt tctgatgccc gccatcaccg
     6481 ccgtgctgct tggcggtgcc aatatttatg gtggttccgg ttccattatc ggcaccgcca
     6541 ttgcggtttt attagtggga tatttgcaac aaggtttgca aatggcagga gtgccaaatc
     6601 aggtgtccag cgccctttcc ggtgcgctac ttatcgtcgt tgtcgtaggt cgttccgtta
     6661 gcctgcatcg ccagcaaatt aaagagtggc tggcgcgtcg ggccaataac ccattgccat
     6721 aaaggatatc ttcatgacac ttcatcgctt taagaaaatc gccttactta gcgctcttgg
     6781 cattgccgca atctctatga atgtgcaggc cgcagagcgt attgcattta ttcccaaact
     6841 ggttggcgtg ggatttttta ccagcggtgg caacggcgca caacaagcgg gtaaagagct
     6901 gagcgttgat gtgacctacg acgggccgac agaacccagt gtttctggtc aggtacagtt
     6961 gattaataac ttcgtcaatc aaggttataa cgccattatc gtttctgcgg ttacgcctga
     7021 tggcttgtgt ccggcactga aacgcgccat gcaacgtggt gtgagagtgc tgacctggga
     7081 ctctgatact aaaccggagt gccgctctta ctacattaat cagggaacgc ccgcccagtt
     7141 gggaggtatg ttggtggata tggcggcgcg tcaggtgaat aaagacaaag ccaaagtcgc
     7201 gtttttctac tcaagcccca ccgttacgga ccaaaaccag tgggtgaaag aagcgaaagc
     7261 gaaaatcgcc aaagagcatc ctggctggga aattgtcact acgcagtttg gctataacga
     7321 tgccactaaa tcattacaaa ccgcagaagg aatattaaaa gcgtatagcg atctcgacgc
     7381 cattatcgcc cccgacgcca acgccctgcc cgctgccgca caagccgcag aaaacctgaa
     7441 aaatgacaaa gttgcgattg tcggattcag tacgccaaac gtgatgcgtc catatgtgga
     7501 acgcggcacg gtgaaagaat ttggcctgtg ggatgtggtt cagcaaggca aaatttcagt
     7561 gtatgtcgcg gatgcattat tgaaaaaagg atcaatgaaa acgggcgaca agctggatat
     7621 ccagggcgta ggtcaggtgg aagtctcgcc aaacagcgtt cagggctatg actacgaagc
     7681 ggatggtaat ggcatcgtac tgttaccgga gcgcgtgata ttcaacaaag agaatatcgg
     7741 caaatacgat ttctgatgtg cattacttaa ccggagtaag ttatggcaga tttagacgat
     7801 attaaagatg gtaaagattt tcgtaccgat caaccgcaac aaaatatccc ttttatcctg
     7861 aaaggttgcg gtgcgctgga ttggggaatg cagtcacgct tatcgcggat atttaatccg
     7921 aaaacgggta aaaccgtgat gctggccttt gaccatggtt attttcaggg gccgactacc
     7981 ggacttgaac gcattgatat aaatatcgcc ccgctgtttg aacatgccga tgtattaatg
     8041 tgtacgcgcg gcattttgcg cagcgtagtt cccccagcga ccaataagcc ggtggtactg
     8101 cgggcgtcag gtgcgaactc tattctggcg gaattaagta atgaagccgt ggcgttatcg
     8161 atggatgatg ccgtgcgcct gaacagttgc gcggtggcgg cgcaggttta tatcggcagc
     8221 gaatatgaac atcagtcgat caaaaatatt attcagctgg tcgatgccgg aatgaaagtg
     8281 ggaatgccga ccatggccgt gactggcgtg ggcaaagata tggtgcgcga tcagcgttat
     8341 ttctcgctcg cgactcgaat cgccgctgaa atgggggcgc aaattatcaa aacctattat
     8401 gtcgaaaaag gttttgaacg gattgttgcc ggatgtccgg tacccattgt tattgctggc
     8461 ggtaaaaaat taccggagcg cgaggcgctg gaaatttgct ggcaggctat cgatcagggc
     8521 gcttctggtg tggatatggg gcgtaatatt ttccagtctg accatccggt ggcgatgatg
     8581 aaagccgtac aggcggtggt tcaccataac gaaacggctg atcgggcata tgaactctat
     8641 ctgagtgaaa aacagtaact gcggatttaa ggagaagaat tatgcacgtc acactggttg
     8701 aaattaacgt tcatgaagac aagattgacg agtttatcga agtttttcgc cagaaccacc
     8761 tgggctctgt acaggaagaa ggcaatttgc gcttcgatgt cttacaggac ccggaagtga
     8821 attcgcgctt ttatatctac gaagcctata aagatgaaga cgcagtggca ttccataaaa
     8881 ccacgcccca ctacaaaacc tgtgtcgcga aactggaatc tttaatgacc gggccgcgta
     8941 aaaaacgtct gttcaatggg ttgatgccgt gaggcgaatt tatcattttt atctacaatt
     9001 ggggtaacgc gctgacggga gtaaataaat gtctgactgg aacccctctt tatatgtgca
     9061 ctttgccgct gaacgatcgc gtccggcggt ggagctgctt gccagagtac cgctggagaa
     9121 tatcgaatac gttgccgatc ttggctgtgg cccaggtaac agcaccgccc tgctgcatca
     9181 acgttggcct gcggccagga taacaggcat cgactcgtct ccggcgatga ttgctgaagc
     9241 gcgcagtgct ttgccagact gccagttcgt ggaagcagac atccgcaact ggcaaccgga
     9301 acaggcactc gatctgattt ttgctaatgc ctcactgcaa tggctgcccg accactacga
     9361 attgtttcct catctggttt ctttacttaa tccgcagggc gtgctggcag tacagatgcc
     9421 agataactgg ctggagccga cccatgtgct catgcgcgaa gttgcctggg aacaaaacta
     9481 cccggatcgc gggcgtgagc cgttggctgg cgttcatgct tactacgata ttttgagcga
     9541 agccggatgt gaggtcgata tctggcgaac cacctactat caccagatgc cgtcacacca
     9601 ggcgattatc gattgggtga ctgccactgg attacgtccg tggttacagg atctgaccga
     9661 gagcgaacag cagctttttc ttaagcgcta ccatcagatg ctggaagagc agtatccact
     9721 gcaagagaac ggacagatac tgctggcatt tccgcgtctt tttattgttg cccggcgtat
     9781 ggagtaaata tcacgtcagc tggtaatgac gatcgggaag aatttttgct ggaatttcgg
     9841 cttcatcgtt catctgtaac aggtcaattt caatagcgtt gcagatggca tccagcggta
     9901 aatcattgtt ttcagtaccg aacggatctt ccagttcttc cgccagacaa tccagcgaaa
     9961 taaaagtgta ggaaatcagc acagagataa aaggcgtcat gtaatgcagg tccacgacca
    10021 gcgcgaacgg cagcatgata caaaacagat aaacggtacg atgcaaaatc agggtgtagg
    10081 caaagggaat tggcgtatag gcaatgcgct cgcatcctgc caggaccgct gaaatatcat
    10141 taagccgatc gttgaggcta ataaacagga tatctgaaag ctgcccattg cggcgctgaa
    10201 ccgccaacca ctcccccatt attaacaaga tacggttagc tggagagttc gaagccagta
    10261 caggctgaag atcttcagtc ttgagataat gagccagcac ttccgcctgt ggctgtttgc
    10321 gtaatgtcat gcgtaaacag tgggcgaagg cgatttgcaa ccgggcaaac tcccttacac
    10381 ttgccgaatc cggcaatgtc gtttttacct cgcgcagtaa cgaccgtgag gcaatcatca
    10441 actgccccca aagttttcgc gcttcaacgt aacgggcgta cccggcatta ttacgaaaac
    10501 caagaaaaat ggcgatggcg acaccgagaa tgctgaacgg tgcgagggtg aatttgatgc
    10561 ccagatgcgt gtaccagggc agcatgaaaa taacagcgat agaaaagaga aaattgagta
    10621 gtaagcgcga tgatatcttg gataatactg agccgtgcca gacaaaaata cggcgcagcc
    10681 agtgttgttg tggacgaaca atcatggtta tcttcaggcg tgaaaaagtc gccctattaa
    10741 acgtgattac gatcacattc tcaagacgct acttacaaat tacctacaag cttacaacat
    10801 taacaggcgc tgcatgtggc atctgttaat gataaaggca cataggatgt tgtaactaat
    10861 atagtgaaat aagaaacccg gtcgaaaccg ggttcagaag taacggtgtt attagcacaa
    10921 cggacgtaca gcttcgcgca tccctttttc aagaatcgca tccaggtcat tagcaacctg
    10981 ctcgaccaga ccaggcactt gcgtcaggtc ctgctcccag tggtcttttt ctgccaacac
    11041 aatcgctacc agttcctggg taccgataac acggtcacga tgctggctcc agagttgctg
    11101 ataacgttcc agccagtgtg catcgtcctg taccggatat gtttctccgt tacgctcacc
    11161 acgatagaag gcaatcaatg ccgctaatgc gaaagtaagg cgcgccggaa gtgtgccttg
    11221 tgccttctgc cctgccagca gctgcggcag gatgcgagtg cggaacttgg tcataccgtt
    11281 gagcgcgata gacagcagct gatgcttaat gtacgggtta cggaaacgcc cggtgactgc
    11341 actggcgaaa gattccagtt catcacgagg caaatccagt accggaataa tttcttcgta
    11401 gatagctttt tcaacgaatg cgcaaatttc agcatcgttc atcgcttcac ctacggtatc
    11461 cagccctgcc tggaacgcca ccggcaccag cgcggtgtgc acaccgttga ggattgccac
    11521 tttgcgttct ttgtacggtt taatatcgtc aacaatcagc acgttgagcg gatatttgtc
    11581 cagacgcagt tcagtcgcta aggatttcgg cccctgaatc acaaacaggt aaaagtgttc
    11641 agcggtgtcg aggaaaccat cgtgataacc cagttcttct tccagtttag ccacttcatc
    11701 gcgcggataa ccggtaacga tacggtccac cagcgtagaa cagaagctgt tagcctgatc
    11761 caaccattga ataaatgctt ctggcagtgc ccactcttga gcatagcgca gcaccagttc
    11821 acgcaacgcg tcgccattgt agtcgatcaa ctcacacggg atgatgatcc aacctttatc
    11881 cagcgcaccg ttgaaatggc taaaacgttc gaacagcaga cgggtcagtt ttgcgggata
    11941 gcttaccgct ggcgcgtcat cgaatttatc gcccgcatgg tagctgatac ccgcttcggt
    12001 ggtgttagag aaaacaaagc gcatttccgg attgtgcgcc agtttcagga attcatcgta
    12061 ttcactgtag acgctgattt cacgattaac agagcgaatc agacgcgcgt cgctgaccgc
    12121 ttcccccttc tcattcaggc cacggataat ggtggtgtac agaccatcct gcgtgctcag
    12181 tgacggcggg aatgaagttt caatcggacg aacaacgacc acgccagaat tcagatcggt
    12241 gtgctcattc aggagatcga tttgccagtc aacaaaggcg cgcaggaagt taccttcacc
    12301 aaactgaatg atacgttctg gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt
    12361 cacaatgggt tcccttctga ttagtcatac aacctgtttg aattggtacg acaggttagc
    12421 aaactttaat acgccgaacc cctgttttga tcaactcctg atgattaatg agcagtttta
    12481 tgagaaaagt gtggcgcgga tcatggttta atcgaggaaa tacgcctttt cctggatcat
    12541 aaagtggtag aacacattgc attcaaatcg cgcggaatga ataaagatgt cagacaactt
    12601 cctcaccgta acgcatagtg ctggtacggt tgcgcccatc tttcttcgag cgatacagac
    12661 acgtatcggc ttcgaccatc aatttattaa aatcatcggt cagcgttcgg tacgatgcgc
    12721 gaccactacc gacgccaata cttaccgtga gataaagcgt tttttgttgc caggtgaatg
    12781 gttgcagttc aacgccttta cggatttttt ccgccattag cagaccatcg acaggattca
    12841 ccgacggcac tgcaacagca aattcttcgc cgcccatgcg cgccaccagc cccttatcac
    12901 cgacaatctt ctgaatatgc cgggcaaaca cgcttaacac tttatcgcca cattcatgtc
    12961 catagttatc gttaatgctt ttgaaatagt cgatatcaag cagcatgaca gtcagatgtt
    13021 gtgtctgttt cagcgatgga cttttcagcg cctcataaag accggaccgt gagtaaacct
    13081 gagtcagaaa atcaaagtcg gctcgcagcg caacttgctt cattagcgaa ttgatcgctg
    13141 ccacgctaaa agaaaccata attgggcata tcgccatcgt ggcaataccg agacgtgcgg
    13201 agaacatttg cggaatggag aacggcgaac cgaccgagat atcaatcacc gaatttgcca
    13261 ccagtacgat ttccaccgca ccggtgacaa aggtcaacag acatgttacc tgcggcgtat
    13321 agcgcactgc acaccagatt aatgcaggca gcgggaacgc cagactcccc gctccgccaa
    13381 tgactaccga ggcaataacc gacacaataa gcgcgatggc gggcatcatc tgttctgctt
    13441 taaagcgcgg cagcactcca ggaatagcca acgtcagcat acaaggcacg atcaaaacgc
    13501 ccgttgagaa ttgctcactg aaccagtccg caagcaaagg ccagaaatcc agactatcaa
    13561 tactgaccga accaatcgcc ccgacaatag cgcataataa tgcggcaatc agacagtaat
    13621 taaatagccg taaggcactt actggctcat acttattttt ccccaaacgc ttatcccgca
    13681 cgaccagtaa ggcgacggta acaataaaca tcatattgga gaaattgata gccagtgaaa
    13741 ccagtcccca ttcggtggtt atggcatcat aaaccagcat cgccacataa ctgatcgcat
    13801 agtaatgcag tcgattaagc cagacatagc gggcaaacac ccccgccatt acgccattca
    13861 gcggccagaa aagagaaagt tcgtgaacca gccgtaactc tgcaccaata aaatagaaca
    13921 gcgtggttag cgcaaaaata gcgatgctat tacgtagcag atgaccttcc tggaacaaac
    13981 ggaaagttga aatggggtgt acatgcatta attatctgtc catacaattg cgggtggcaa
    14041 ttatctcaac gtaataagta aattctcata aaggatagca tgttcgctga cagcggcggc
    14101 aaataaaggt accctgggaa tgctatttta tcgttttccg caattctcat ggctgattca
    14161 tctgtacaac cagccagtga tagattaccg ccacgatata acgttgttgt tcttcgctta
    14221 acgaaacacc ctgcggaatg ttatgccact tagccagaca accgcgacaa caggttgcag
    14281 tggcgtgctg agcgataaac actggatgcc cgcgcatggg ggtttgctta ccgtcattag
    14341 ccggtaatgc cggagcaaga cgtttagcga caaaatccgc cgcatgttgt tcgatgactg
    14401 gcgcgccttt ctccagacaa tactgacgct ctttcatgcc caaacgaaag cgagatctaa
    14461 attttgaacg ggataaacgc gcgaagagtg gatcgagaga ctgcattaat aaaccgaacg
    14521 ccctaactgt ttcgttaatt gttcaagaac ggcaatgccc gcaagcgagt tacctgcatc
    14581 atccagttcc ggactccaga ctgcgatggc catttcatgc ggaacaatcg ccacaatacc
    14641 gccaccaacg ccagatttcg ccggtagtcc cacccgccag gcaaactccc ccgcgttctg
    14701 gtacatacca ctggtcgcca tcagcgcgtt aatttgccgc gcctgcattg gcgtcaccac
    14761 aggttcatca atatgaatag ctttcccctg attagccaga aagacaaacg tccgggccag
    14821 ctctacacag ctcattttca gagcgcagta atgaaagtag ttttgcagaa cggttgtcac
    14881 gtcatgatgg aaattgccaa acgacttcat cagccaggcg atagccgcat ttcgcgcgga
    14941 atgttcaaat tcggaacgcg ctaccaccgt atcgtatgaa atatcagaca caccgcttaa
    15001 gccgcgcacg acttccagca tacgttgccg tggtgcgctt agtcgccctt gcaacatatc
    15061 gcagaccacc agcgcaccgg cattaatgaa cggattacgc ggaataccct gttccatttc
    15121 cagttgcact aaagaattga acggtgatcc agacggatct ttgccgacgc gttgccagat
    15181 ttcctcttcg gagtaatgac gcatggcgac aacgagactc agcactttgg aaatagactg
    15241 aatggaaaaa cgttcttgcg cgtctccggc ctgaaaaagc tgtccgtcaa cggtacagat
    15301 agcaatgccc aatcgggaac cgtctactgt agccagcgcc ggaatataat ccgcgacttt
    15361 accctgacca atgagcggcc gcacttgccg caagatgttt tctaaaattg cattatccat
    15421 ggcgactgcc actttctact cctggaccgc aggtctgaaa agacctgcga gtatatcaga
    15481 gctgaatatg tcgcgtcaga tccggtcttt ccacaccgtc tggatattac agaattcgtg
    15541 taagccgaaa tgggaaagct cacgaccaaa gccactcttt ttcacgccac caaaggccac
    15601 tcgcgcgtcg ctggcacaat aaccattgat aaacacccca ccgcattcca gacgtgccgc
    15661 catctgtctg gcctgtgttt cgtcggtggt aaaaatggtc gctgaaaggc cgaactcact
    15721 atcattagcc agttccagcg catgttctgc atctttcgca acggtgattg ccgcaacagg
    15781 gccaaacatt tcttcccgaa acgcggtcat ttctggggta acattcgcca gaaccgttgg
    15841 cggatagtag ttacctgccc cagccatctt ttccccgccc agtaacaaac gcgcaccctg
    15901 cgccagggtt ttctccacct gatgatgcag ctcatcacgt aaatcaaaac gagccattgg
    15961 tccgagagca ttctcttcat cacggggatc gcccattttc aaggctgccg cagctgccac
    16021 aaaacgttcg gtaaatgcag aagcaattcc ctcttcgata ataaagcgtt ttgccgctgc
    16081 acaaacctgt ccggtattct gataacgtcc ggctaccgcc gctttaaccg ccagttccag
    16141 atcggcatcg ttaagcacaa taaatggatc cgaaccgccc agttccagta cgcatttttt
    16201 cagtgccgct ccagcctgtg cgccaatagc cgctcccgca cgaacacttc cggtcaccgt
    16261 gacagcagca atgcgcgaat ctttaatcat ttgactgaca ccgtcgttgt cggcattcag
    16321 ccagccatat acgccttgcg ggattcccgc atctttaaac acctgggcaa tgagctgagc
    16381 acagcccatc acattcggcg catgtttaag taagtagccg ttacctgcaa gaatgatggg
    16441 aaccgcgcca cgcatcacct gccataacgg aaagttccac ggcataatcg ccagaatcgt
    16501 ccccaacggt cgatactcaa taactgcctg ctgattttcc accagcgtag gttccgcctt
    16561 cagcattgcc ggaccatgtt ctgcatacca gtcacacaaa ttcgccgatt tcgccacttc
    16621 agcgcgcgcc tgattgattg gtttgcccat ttcacgggtg atcatttgcg ccatttcttc
    16681 gctacgggcg cgcagagcct taccgatacc acgcagtttt tcagcacgat aatctatatt
    16741 tgtctcgcgc cagtcgcgaa agcctgctgc cgccagctga agtgcgtttt cgatatcgtt
    16801 agcgccagcc caaggcagca cagaaagttg ttcacccgtg gcaggattta ttgaaattgc
    16861 atgagttgcc ggagtaatgg tcatcggggt atctccttta tgagtcatgg tatgaagata
    16921 cgcagattta ctcttgcttt aaaatgaata atattaagcc acttattcac gaatcgagaa
    16981 tgttatggat ctgacccaac tggagatgtt caacgcggtt gccgaagctg gcagcattac
    17041 ccaggctgca gcaaaagtgc atcgcgtgcc gtcgaatctc actacccgtc tacgccagct
    17101 ggaaacagaa ctgggggtag atctgtttat tcgcgagaat cagcgtttac gtctctctcc
    17161 tgccgggcat aactttttac gctatagcca acaaattctc gcgttagtgg atgaagcgcg
    17221 gagcgttgtc gctggcgatg agccgcaagg tttattttct cttggttcgc tggaaagcac
    17281 cgcagcagtg cgcattccgg ccacgctggc ggaattcaac catcgttatc ccaaaattca
    17341 gttttcactt tccaccggcc cttccggcac tatgctggaa ggagtgctgg agggaaaact
    17401 gaatgcggca tttattgatg ggcccattaa ccatactgcc attgacggga taccggtata
    17461 ccgcgaggag ctgatgatcg tcacgccaca aggtcatgcg ccagtaatcc gcgccagtca
    17521 ggttaacggc agtaacattt atgccttccg cgccaattgt tcgtatcgtc gccacttcga
    17581 gagctggttt catgctgacg gtgccgctcc gggaactatt cacgagatgg agtcgtatca
    17641 cgggatgttg gcctgtgtgg tcgcaggagc aggcattgcg cttattccac gctctatgct
    17701 ggaaagtatg ccggggcatc accaggttga agcgtggccg ttagctgagc aatggcgttg
    17761 gttaacaacc tggctggtct ggcgtcgtgg tgcgaaaacg cgtccgcttg aggcatttat
    17821 tcaactactg gatgtgcctg actcggcaaa acagggttac caatgagcta tttttgatag
    17881 ttcttgcagc tcaaatgccc tatatagtga tgttccgatg acttatgact atatggggca
    17941 aatatggtta cgccagtaag catcagcaat tatatatctc ttcctgatga ttttcccgct
    18001 cgtaatattg cacctcaggt aaaagaagtt ttaaaggatt ttattgatgc actcagtacc
    18061 ataatatgtg atgaagagtg gcgcacgtct ttaaacatca actcagccac aaaaaagata
    18121 tttaataacc ttgataactt atcatatatt cagaggactt ctttccgggg taacgacacg
    18181 ctatacaatg aaaaggttca gtttaaactc acttatcccg tcaagaatgg aagacacaaa
    18241 gaaaatattg aatttcaggt agtaataaat ttaagcccta tttatttaga taattttcgc
    18301 catgatggag aaattaatat tttttgcgct cccaacccaa
//
LOCUS       DAZGAN020000095        18014 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGAN020000095 DAZGAN020000000
VERSION     DAZGAN020000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.095"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..276
                     /locus_tag="ACHKYO_RS18080"
                     /old_locus_tag="pgaptmp_003616"
     CDS             <1..276
                     /locus_tag="ACHKYO_RS18080"
                     /old_locus_tag="pgaptmp_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HGU4430392.1"
                     /translation="ILFTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGI
                     SSVQQEEYYIVFGALSLKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            295..993
                     /locus_tag="ACHKYO_RS18085"
                     /old_locus_tag="pgaptmp_003617"
     CDS             295..993
                     /locus_tag="ACHKYO_RS18085"
                     /old_locus_tag="pgaptmp_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HGU4430393.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            1044..1703
                     /locus_tag="ACHKYO_RS18090"
                     /old_locus_tag="pgaptmp_003618"
     CDS             1044..1703
                     /locus_tag="ACHKYO_RS18090"
                     /old_locus_tag="pgaptmp_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HGU4430394.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            1721..2119
                     /locus_tag="ACHKYO_RS18095"
                     /old_locus_tag="pgaptmp_003619"
     CDS             1721..2119
                     /locus_tag="ACHKYO_RS18095"
                     /old_locus_tag="pgaptmp_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HGU4430395.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2129..2767
                     /locus_tag="ACHKYO_RS18100"
                     /old_locus_tag="pgaptmp_003620"
     CDS             2129..2767
                     /locus_tag="ACHKYO_RS18100"
                     /old_locus_tag="pgaptmp_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HGU4430396.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2770..3922
                     /locus_tag="ACHKYO_RS18105"
                     /old_locus_tag="pgaptmp_003621"
                     /pseudo
     CDS             2770..3922
                     /locus_tag="ACHKYO_RS18105"
                     /old_locus_tag="pgaptmp_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
     gene            4006..5631
                     /locus_tag="ACHKYO_RS18110"
                     /old_locus_tag="pgaptmp_003622"
     CDS             4006..5631
                     /locus_tag="ACHKYO_RS18110"
                     /old_locus_tag="pgaptmp_003622"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HGU4430397.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELRREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5748..6023)
                     /gene="yjfN"
                     /locus_tag="ACHKYO_RS18115"
                     /old_locus_tag="pgaptmp_003623"
     CDS             complement(5748..6023)
                     /gene="yjfN"
                     /locus_tag="ACHKYO_RS18115"
                     /old_locus_tag="pgaptmp_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HGU4430398.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6172..6501)
                     /gene="bsmA"
                     /locus_tag="ACHKYO_RS18120"
                     /old_locus_tag="pgaptmp_003624"
     CDS             complement(6172..6501)
                     /gene="bsmA"
                     /locus_tag="ACHKYO_RS18120"
                     /old_locus_tag="pgaptmp_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HGU4430399.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6683..7432
                     /gene="yjfP"
                     /locus_tag="ACHKYO_RS18125"
                     /old_locus_tag="pgaptmp_003625"
     CDS             6683..7432
                     /gene="yjfP"
                     /locus_tag="ACHKYO_RS18125"
                     /old_locus_tag="pgaptmp_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4430400.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPTDESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7429..8184)
                     /gene="ulaR"
                     /locus_tag="ACHKYO_RS18130"
                     /old_locus_tag="pgaptmp_003626"
     CDS             complement(7429..8184)
                     /gene="ulaR"
                     /locus_tag="ACHKYO_RS18130"
                     /old_locus_tag="pgaptmp_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HGU4430401.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8292..9356)
                     /gene="ulaG"
                     /locus_tag="ACHKYO_RS18135"
                     /old_locus_tag="pgaptmp_003627"
     CDS             complement(8292..9356)
                     /gene="ulaG"
                     /locus_tag="ACHKYO_RS18135"
                     /old_locus_tag="pgaptmp_003627"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGU4430402.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9711..11108
                     /gene="ulaA"
                     /locus_tag="ACHKYO_RS18140"
                     /old_locus_tag="pgaptmp_003628"
     CDS             9711..11108
                     /gene="ulaA"
                     /locus_tag="ACHKYO_RS18140"
                     /old_locus_tag="pgaptmp_003628"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGU4430403.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11124..11429
                     /gene="ulaB"
                     /locus_tag="ACHKYO_RS18145"
                     /old_locus_tag="pgaptmp_003629"
     CDS             11124..11429
                     /gene="ulaB"
                     /locus_tag="ACHKYO_RS18145"
                     /old_locus_tag="pgaptmp_003629"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HGU4430404.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEIIVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            11439..11903
                     /gene="ulaC"
                     /locus_tag="ACHKYO_RS18150"
                     /old_locus_tag="pgaptmp_003630"
     CDS             11439..11903
                     /gene="ulaC"
                     /locus_tag="ACHKYO_RS18150"
                     /old_locus_tag="pgaptmp_003630"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HGU4430405.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            11917..12567
                     /gene="ulaD"
                     /locus_tag="ACHKYO_RS18155"
                     /old_locus_tag="pgaptmp_003631"
     CDS             11917..12567
                     /gene="ulaD"
                     /locus_tag="ACHKYO_RS18155"
                     /old_locus_tag="pgaptmp_003631"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGU4430406.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12577..13431
                     /gene="ulaE"
                     /locus_tag="ACHKYO_RS18160"
                     /old_locus_tag="pgaptmp_003632"
     CDS             12577..13431
                     /gene="ulaE"
                     /locus_tag="ACHKYO_RS18160"
                     /old_locus_tag="pgaptmp_003632"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HGU4430407.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13431..14117
                     /gene="ulaF"
                     /locus_tag="ACHKYO_RS18165"
                     /old_locus_tag="pgaptmp_003633"
     CDS             13431..14117
                     /gene="ulaF"
                     /locus_tag="ACHKYO_RS18165"
                     /old_locus_tag="pgaptmp_003633"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HGU4430408.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIASFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14246..14521)
                     /gene="yjfY"
                     /locus_tag="ACHKYO_RS18170"
                     /old_locus_tag="pgaptmp_003634"
     CDS             complement(14246..14521)
                     /gene="yjfY"
                     /locus_tag="ACHKYO_RS18170"
                     /old_locus_tag="pgaptmp_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HGU4430409.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            14848..15243
                     /gene="rpsF"
                     /locus_tag="ACHKYO_RS18175"
                     /old_locus_tag="pgaptmp_003635"
     CDS             14848..15243
                     /gene="rpsF"
                     /locus_tag="ACHKYO_RS18175"
                     /old_locus_tag="pgaptmp_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGU4430410.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15250..15564
                     /gene="priB"
                     /locus_tag="ACHKYO_RS18180"
                     /old_locus_tag="pgaptmp_003636"
     CDS             15250..15564
                     /gene="priB"
                     /locus_tag="ACHKYO_RS18180"
                     /old_locus_tag="pgaptmp_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HGU4430411.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15569..15796
                     /gene="rpsR"
                     /locus_tag="ACHKYO_RS18185"
                     /old_locus_tag="pgaptmp_003637"
     CDS             15569..15796
                     /gene="rpsR"
                     /locus_tag="ACHKYO_RS18185"
                     /old_locus_tag="pgaptmp_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGU4430412.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            15838..16287
                     /gene="rplI"
                     /locus_tag="ACHKYO_RS18190"
                     /old_locus_tag="pgaptmp_003638"
     CDS             15838..16287
                     /gene="rplI"
                     /locus_tag="ACHKYO_RS18190"
                     /old_locus_tag="pgaptmp_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGU4430413.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(16401..17372)
                     /locus_tag="ACHKYO_RS18195"
                     /old_locus_tag="pgaptmp_003639"
     CDS             complement(16401..17372)
                     /locus_tag="ACHKYO_RS18195"
                     /old_locus_tag="pgaptmp_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HGU4430414.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQEVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(17425..>18014)
                     /locus_tag="ACHKYO_RS18200"
                     /old_locus_tag="pgaptmp_003640"
     CDS             complement(17425..>18014)
                     /locus_tag="ACHKYO_RS18200"
                     /old_locus_tag="pgaptmp_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HGU4430415.1"
                     /translation="SGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFLESVITT
                     AIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDMT
                     PAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPINK
                     IIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
BASE COUNT         4370 a         4323 c         4745 g         4576 t
ORIGIN      
        1 attcttttta cttcccgcca aatgattatc gaaaccttta tttgtccggt aagtagtatc
       61 agcaatccag atgaatttaa taccttctta ttaagaaatc agaagatgat gccgttgtca
      121 tcggtaggaa tctccagcgt gcaacaggaa gagtattaca ttgttttcgg ggcgttatcg
      181 cttaaatctt ctcttgaaga tatcctgctt gagataacct cgctggtaga taacgcattg
      241 gatctggctg aaattacaga agaatattca cactaattac aaggacgggt atttatggga
      301 attttaaaaa gtttatttac gctggggaaa tcgtttatct cccaggcgga agaatccatt
      361 gaagaaaccc agggcgtgcg catgctggaa cagcatattc gtgacgctaa agctgaactc
      421 gataaagccg gaaaatctcg cgttgatctg ctggcgcggg tgaaattaag tcacgataag
      481 ctgaaagatt tacgtgagcg caaagccagt ctggaagccc gtgcgctgga agcgttaagc
      541 aagaacgtta atccgtcgtt gattaacgaa gttgctgaag aaatcgcgcg ccttgagaat
      601 ctcattaccg ctgaagagca agtgttgtcg aatctggaag tctcccgtga tggcgtggaa
      661 aaagcagtta cagcgacagc gcagcgtatc gctcagtttg agcagcaaat ggaagtcgtt
      721 aaagccactg aagccatgca gcgtgcacaa caggctgtaa caacctctac cgttggcgca
      781 tcttccagcg tttcgacagc ggcagaatcc ttaaaacgcc tgcaaacgcg tcaggccgaa
      841 cgtcaggctc gcctggatgc tgctgcacag ttggaaaaag tcgcagacgg tcgcgatctt
      901 gatgaaaagc tggcggaagc cggaattggc ggtagcaata aaagtagcgc ccaggatgta
      961 ttagcaagac tgcaacgcca acagggcgag taattttttt gccagcctcg ctaaaaggct
     1021 ggcaactatt ttaaggataa aatatgtctg gttttttcca gcgtctgttt ggcaaggata
     1081 ataagcctgc tatcgctcgc ggtccgctgg gacttcatct caatagtggc tttacgctcg
     1141 acacgttagc gtttcgtttg ctggaagatg aattgctgat tgcgctgccg ggtgaggaat
     1201 ttacggtagc cgctgtcagc cacatcgatc tgggcggcgg tagtcagatt ttccgctact
     1261 acacttcagg cgatgaattt ctgcaaatca ataccaccgg cggcgaagat attgatgaca
     1321 ttgatgatat caagctcttt gtctatgaag agagctacgg tatcagtaaa gagagtcact
     1381 ggcgcgaggc gatcaacgcc aaagtgatgg gggcaatgat cttaaactgg caggaaaaac
     1441 gctggcagcg attttttaac agcgaagaac cgggaaatat cgaacccgtc tacatgctgg
     1501 aaaaagtaga aaatcaaaac catgccaaat gggaggtcca taattttacc atgggctacc
     1561 aacgccaagt aaccgaagat acttacgaat atttgctgtt aaatggtgag gaatctttta
     1621 acgatctcgg cgagccagag tggttatttt cgcgcgcgtt gggtgtcgat atcccgctga
     1681 catcacttca tattattggt taattacaag gacgtttaca atgcatatac tggattcact
     1741 tcttgccttt agtgcctatt tttttattgg cgtggctatg gtgattattt ttctgtttat
     1801 ctactccaaa attacaccgc acaacgaatg gcaattaatc aaaaacaata ataccgcggc
     1861 gtcactggca ttcagcggta cattgttggg ttacgtgatc cccttatcca gtgcggcaat
     1921 caatgcggtg agtattccag actatttcgc ctggggcggg attgcactgg tgattcagtt
     1981 actcgttttt gctggtgtca gactttatat gcccgcatta agcgaaaaaa ttattaatca
     2041 caataccgca gcaggaatgt ttatgggaac cgccgcactg gctggtggta tttttaacgc
     2101 agcttgtatg acatggtaat ggacgatcat ggccagaaaa cgcaaatcaa gaaataacag
     2161 taaaattggt cacggagcga tcagtcgcat tggaagaccg cataatcctt ttgagccgcg
     2221 ccgcaatcgc tacgcacaaa aatacttaac gttggtgcta atgggcggtg ccgttttttt
     2281 aatagtcaaa ggatggagtg aaagtggcga tgtcgataac gacggcgacg gaacgtttta
     2341 ttcgacagtg caggattgta ttgatgacgg taataatgcc gacatttgcg cgcgtggctg
     2401 gaacaacgcc aaagcggcat tttatgccga tgttccgaag aatatgactc agcagaattg
     2461 tcagtctaag tacgaaaatt gctactacga caatgttgaa cagagttgga tcccggtcgt
     2521 ttctggattt ttgttaagcc gagttattcg taaagatcgc gatgagccgt tcgtctataa
     2581 cagcggcgga tcctcatttg cttcccgccc cgtctggcgc aacacttccg gtgattactc
     2641 ctggcgctcc ggttctggca aaaaagagtc ttactcttca ggcggcttta ccaccaaaaa
     2701 agcgtcaacc gtttcgcgcg gcggctatgg tcgttcttcc agcgcccgtg ggcattgggg
     2761 aggctaatca tgctgagaca caacgttcct gtgcgacggg atctggacca gatcgccgct
     2821 aacaacggtt tcgactttca tatcatcgac aatgaaatct attgggacga gagtcgggct
     2881 taccgtttta ctctgcgcca gattgaagag cagatcgaaa aaccgactgc ggaactgcat
     2941 cagatgtgcc ttgaggtggt ggatcgcgcg gttaaagacg aagagatcct gacgcaactg
     3001 gcgatcccgc cgttgtactg ggatgtgatc gccgaaagct ggcgcgcacg cgatccttcg
     3061 ctgtatggcc ggatggattt tgcctggtgt ggcaatgcgc cggtgaagct gctggagtac
     3121 aacgccgata cgccgacctc gttgtatgag tcggcttatt tccagtggtt gtggctggag
     3181 gatgcccggc gcagcggcat tattccgcgt gatgccgatc agtacaatgc cattcaggaa
     3241 cgcctgattt cacgctttag cgagctttat agccgggagc cgttttattt ctgctgctgt
     3301 caggacaccg atgaagacag gactaccgtg ctgtacttgc aggactgcgc ccagcaggca
     3361 gggcaggagt cgcggtttat ctacattgaa gatctcggtt tgggcgtcgg cggtgtactg
     3421 accgatcttg atgataatgt catccagcgt gcatttaagc tgtatccgct ggagtggatg
     3481 atgcgtgacg ataacggtcc gctgctgtgc aagcgtcgcg agcaatgggt ggagccgtta
     3541 tggaaaagta tcttgagtaa taaagggcta atgccgctgc ttccctggtc atcctaacct
     3601 tcttgcatcc tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg
     3661 taaaccgatc tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt
     3721 cgttgaccac gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc
     3781 tctgccgcga tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc
     3841 gtgcggaatg gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt
     3901 tccgcattac attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg
     3961 cagtgaccat actaatggtg actgccattg atggagggag acacagtgca ctggcaaact
     4021 cacaccgttt ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc
     4081 gcgctctgcg aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt
     4141 attggtcagc agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtgaat
     4201 ccgcctgaat tattgcgcta cgatgcgcaa ggacgccgtc tggacgatgt gcgttttcac
     4261 cccgcctggc acctgctgat gcaggcgcta tgtaccaatc gggtgcacaa tcttgcctgg
     4321 gaagaagacg ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttgcacgca
     4381 caggttgagg cagggtcgtt atgtccgata accatgacct ttgccgccac gccattgctg
     4441 ttacagatgt tacccgcgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac
     4501 gattctcact tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg
     4561 gaaaagcagg gcggttccga tgtcatgagc aacaccaccc gcgcagagcg cctggaagat
     4621 ggctcttatc ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat
     4681 ctggtgctgg cgcagactac gggcggtttg tcctgctttt ttgtgccgcg ctttttgcct
     4741 gacgggcaac gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct
     4801 aacgccagct gcgaagtgga gtttcaggat gccattggct ggttgttggg gcaggaaggg
     4861 gaaggaattc gtctgatcct gaaaatgggt gggatgacgc gttttgattg cgccctgggt
     4921 agccatgcca tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat
     4981 gtttttggta atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca
     5041 cttcagcttg aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt
     5101 gccgatgcca aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc
     5161 tgcaaaagag gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat
     5221 tgcgaggaga gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa
     5281 ggttccggca atattatgtg cctggatgtg ctgcgcgttc tcaataagca agcgggcgta
     5341 tacgacttat tgtcggaagc gtttgtggaa gtgaaagggc aggatcgcta ttttgatcgc
     5401 gcggttcgtc gtttacagca gcagctgcgt aagccagctg aagaactgag gcgagagatt
     5461 actcatcagc tattcctgct gggctgcggt gcgcaaatgt tgaaatatgc ttctccgcca
     5521 atggcgcagg cgtggtgtca ggtgatgtta gatacgcgcg gcggcgtacg gttgtcagag
     5581 cagatccaga atgatttatt gctgcgggcg acggggggag tgtgtgtgta agcgtatacg
     5641 actgatgcga cgctggtttc gattaactaa atgaaatatg tgaaaattgt aggccggaca
     5701 aggcgctcgc gccgcatccg gcattgttca tttgctgtag atgacactca tgcatacagt
     5761 atcgcctgta cgcgccagtt atcaggctgc tggtcttcgt acatggtaat aatgcggtac
     5821 catgacgcac cttgttcatc ggcctttaac gccacgatac gttccacgtc ctgcggatcc
     5881 cctgaaatat tgctaactga gatctgacct atttcattca ggcccgtcac gcagtcagca
     5941 ctggcgaatt ctgcagactg tgccgtggcg ctcgcaggat aagtcactaa ttgcagcgag
     6001 ggtgaggcaa gtaattgttt catcgtcagc tccatttttc gtttccccac atgttgcagg
     6061 gcattccatc gcggtaataa acgcccaggc ttccagccgg taatttattg tgcacaggta
     6121 aacgcatagg gacgcaaagc aatgtaaagc tgactatgta agttgtctga tttatttacg
     6181 gtacaaaatg gcttgtgaat accactgtcc tgtcacgatg gtttcgtcca ccatgacgac
     6241 aacgtaataa tcagcttttg cagcgacagc tttcgctctg atttctgcta atgcgtcatc
     6301 cggagaaccc cgaaccatcg tgcttacgct gcctattcgc tgtaaccctt gcgtctggtc
     6361 gcggcgaatc tcttgcggat ggtccgttac tggcggtgct ggctgtggcg taccttgcag
     6421 tgcgctacag gcacttaaca tcaacaccaa taataaactg gcaaaccggt aaataacgct
     6481 attacgtttc ctgctaacca tagtgtagtg ccttattaat ttaactttgg gggaatgttc
     6541 ccgaattgtt acctggtacg cgattttcga atgaaaacgt gacgaaagcg taacccacat
     6601 ctcaacatta tgaactaata ccgccagcag aaacttgtgc taaggatttc ttcatgtcat
     6661 accagacaaa aaggagagac agatgattga aatagaatca cgcgagctgg cagatattcc
     6721 cgttcttcat gcttatcctg tcgggcaaaa agataccccg ttaccgtgcg taatttttta
     6781 tcacggcttt acttcatcca gtctggtgta tagctatttt gccgttgcgc tggcgcaggc
     6841 tggtttgcga gtgatcatgc cggatgcgcc cgatcacggt agccgtttta gtggtgacgc
     6901 agcgcggcgg ttaaatcaat tctggcaaat cttgctacaa agtatgcagg aattcactac
     6961 tttacgtgcg gcaatagccg aagaaaactg gctgcttgat gaccgtctgg cagtcggtgg
     7021 tgcgtcgatg ggcgcgatga cggcactggg gattaccgct cgccacccca cggtgagatg
     7081 taccgccagc atgatgggat cgggctattt tacatcactc gcccgttcac tgtttccacc
     7141 gctgatacct gaaacgacag cacagcagaa tgaattcaat aacattgtcg cgccgctggc
     7201 agagtgggaa gcgacaaacc acctggaaca acttagtgac agacctctac tgctgtggca
     7261 tggcctcgac gatgacgttg tgcctaccga cgaatcacta cgtttgcagc aggccttaag
     7321 cgagacggga cgggataaac tgctaacctg ttcatggcag ccaggcgtgc gtcaccgcat
     7381 tacgcctgag gcgttagatg ctgcagtgac atttttccgc cagcatcttt aaacacgcag
     7441 aatgctgacc ccttgcgctt ccagttgttg caggatttcc gggttagcat ttttgccggt
     7501 gatcagcata tcaatttgat cggcacggct aaaaagcatt cccgcgcgtt cgccaatctt
     7561 actgctatca accagtacca ccagtttccc taccacgctc agcatcttct gctctgccat
     7621 tgccgtcagc atatcggttt tatacaaccc ttctgctgtc agtcctttgc cgctggtaaa
     7681 catccagtgc ccggcataga gactgttttc gctgccctgc gggcttaaag tgatggactg
     7741 acttttgttg tactgtccgc ccataatgat cacgctgtca tgttcttgat cgatcaggta
     7801 atttgccagc ggtagataat tagtgatgat ttgcactggc ttgccacaca tttcccgccc
     7861 aagcagaaac gcggtggagc cgcagttgat gactacgctt tcgcccggat taaccagctg
     7921 cgaggccgct ttagcgatac gtactttttc atcgtgattc tgcgcctgat gcagattcat
     7981 cggcgtccag cgcgggcgct gttgggtaat agcttctgcg ccattgcgca cttttttcag
     8041 tttgccgctt tcgtcaagtt tattgatatc gcgtcgcgca gtggcaggcg aaattcccag
     8101 acgctcaacg actttctcaa cggtcacaaa gcccaattgt gcgagcattt ccaggaggat
     8161 ttgatgtctt tgtgcttcag tcatgagcta ttccgataaa atttgatttt tttagatgat
     8221 atttgaaata gccaggaaat actacgccgg atagcgcgaa cttctccacg ctatccggta
     8281 taaacgacag attacaggaa tgacttgaac ggcagatccg gttcaatagt gaagcaatca
     8341 tcgaaaccgc gcggatagtg gtactcgaag ttgtctttat ccagcggcca ggtaaatttg
     8401 ccgccaacct gccagataaa cggcttgaag ccatacttca gacgatcttt tttcatctcc
     8461 cacagcacgc ggatctcttg cggatcggcc tggaagtttg accagatgtc gtggtggaac
     8521 gggataacca ctttcgcatt cagggcttca cccatacgca gcatatcggc gctggtcatt
     8581 ttgtcggtaa taccgcgcgg gttctcacca tacgatccta acgccacatc gatctgatgt
     8641 tcgttaccgt gcttcgcata atagttagag tagtgggaat cgccactgtg atatagggaa
     8701 ccgccagggg ttttgaacag gtagtttact gcgcgatcgt ccatgccatc tggcagtacg
     8761 ccagccgctt tttgatcggc aggcagggtg atcagtgcag tacggtcgaa agcgtcaagc
     8821 gcatgaattt caatgtcttt cacttttact acatcgcccg gtttgaccac gatgcaacgc
     8881 tctttcggta cgccccagcc aatccacaaa tccacacagg ttttcggtcc aatgaacggt
     8941 acgtcatcag cacagttctg catcacggca gcagcaacgt taacgtcgat atgatcgttg
     9001 tgatcgtgag tcgccagtac cgcgtcgatc tggcgaatcg caaacggatc aagaacaaac
     9061 ggggtggtgc gcaggtttgg ctgcagtttt ttcacgccag ccatgcgctg catctggtga
     9121 ccctgtttca ttaacgggtt accgtgactt tgtttgccag tgccgcacca gaaatcaacg
     9181 caaacgttgg tgccaccttc cgatttcaac cagatcccgg tgcagccaag ccaccacatc
     9241 gcaaatgtgc ctggagcgac ctgttcttgt tcaatttctt cattcaacca gctaccccac
     9301 tccgggaaag tgctcaggat ccaggattca cgggtgatac ttttcacttt actcatcgcc
     9361 atttaccttc atgattgttc aattcgaatc aatgtgtgat tggttttgat taattctgac
     9421 actatttttt caggaaggca atgaccattt tttgactttt tgccagggaa gtggttgttg
     9481 attttgagtg tggaaagatt taatggaatg tgtaattcaa ttaactaaat gaatttaaat
     9541 gaatagttgt ttcgttgtgt gaatcccatt ctatccatgt ggaattattt gcgggtcgcg
     9601 tcacatttaa tcataaataa tcttgttgtg attacttttg aaaattagag tgagtgcaca
     9661 acattccggg tgtgtggaat acccggttac ctcttcttca ggagatcgtt atggagatcc
     9721 tctacaacat ctttaccgtg ttttttaacc aggtcatgac caatgccccg ttgttgctgg
     9781 gtattgtgac ctgtctgggc tacatcctac tgcgcaaaag tgtcagcgtt attattaaag
     9841 gcacgattaa aaccataatt ggtttcatgt tgttgcaggc agggtccggc atcctcacca
     9901 gcaccttcaa accggtggtg gcgaaaatgt ccgaagtcta cggcattaac ggcgcaattt
     9961 ccgatactta cgcgtcgatg atggcaacca tcgaccgcat gggcgatgcc tatagctggg
    10021 taggttacgc ggtgttgtta gcgctggcgc tgaacatctg ttacgtgctg ctgcgtcgca
    10081 ttaccggtat tcgtaccatc atgctgactg gtcatatcat gttccagcag gcggggctga
    10141 ttgccgttac gttgtttatt ttcggctact ccatgtggac aaccattatc tgcacggcga
    10201 ttctggtttc gctctactgg ggcattacct ccaacatgat gtacaagccg actcaggaag
    10261 tgacggacgg ctgcggtttc tccatcggtc accagcaaca gtttgcatca tggattgcct
    10321 ataaagttgc gccgttcctc ggcaaaaaag aggaaagcgt tgaagacctc aaactgccag
    10381 gctggctgaa tattttccac gacaacatcg tctccacggc gattgtgatg accatcttct
    10441 ttggtgccat tctgctctcc ttcggtatcg acaccgtgca ggcgatggca ggcaaagtga
    10501 actggacggt gtacatcctg caaactggtt tctccttcgc ggtggcgatc ttcatcatca
    10561 ctcagggcgt gcgcatgttt gtggcggaac tctctgaagc atttaacggt atctctcagc
    10621 gcctgatccc tggtgcggtt ctggcgattg actgtgcggc tatctatagc ttcgcgccga
    10681 acgccgtggt ttggggcttt atgtggggca ccatcggtca gctgattgcg gttggcatcc
    10741 tggtcgcctg cggttcctcg atcctgatta ttcccggctt tatcccgatg ttcttctcca
    10801 acgccaccat cggcgtgttc gctaaccact tcggcggctg gcgtgcggcg ctgaagatat
    10861 gtctggtgat ggggatgatt gaaatctttg gctgcgtctg ggcggtgaaa ctcaccggta
    10921 tgagcgcctg gatgggcatg gcggactggt cgattctggc accgccgatg atgcagggct
    10981 tcttctccat cggtatcgcc tttatggccg tcatcattgt aattgcactg gcttatatgt
    11041 tcttcgctgg ccgcgcactg cgcgcagaag aagatgcaga aaaacaactg gcagaacagt
    11101 ctgcttaata aggagttttg attatgaccg tacgtattct ggctgtgtgt ggcaacggac
    11161 aaggcagttc catgatcatg aagatgaaag tggaccagtt tttaacccaa tcaaacattg
    11221 accacacggt aaatagctgc gcggttggcg aatacaaaag cgagttgagc ggcgcggata
    11281 tcatcatcgc ttctacgcac attgcgggcg aaatcatcgt gaccggcaac aaatacgtgg
    11341 ttggcgtgcg caacatgctc tctcctgccg actttggccc gaaactgctg gaagtgatca
    11401 aagcgcattt cccgcaggat gtgaagtaag gacgcgccat gaaattacgt gattcgctgg
    11461 cggagaataa atccatccgc ctgcaggctg aagctgagac atggcaggac gcagtaaaaa
    11521 tcggcgttga cctgctggtg gcggcggatg ttgtcgagcc gcgttactac caggcgattc
    11581 tggatgcggt tgaacagcac ggtccgtact ttgtgctcgc accaggcctg gcaatgccgc
    11641 acggtcgccc ggaagagggc gttaagaaaa ccggttttgc gctggtgacg ctgaaaaagc
    11701 cgctggagtt caaccacgaa gataacgacc cggtggatat cctcatcacc atggcggcgg
    11761 tcgatgccaa cacgcaccag gaagtgggca tcatgcagat cgtcaacctg tttgaagatg
    11821 aagaaaattt cgaccgttta cgtgcctgcc gtaccgagca ggaagtactg gatctcattg
    11881 accgcaccaa cgcggcagct taagaaggaa ttgaagatgt cattaccgat gttgcaagtc
    11941 gcgctggaca accagactat ggatagcgcc tacgaaacca ctcgcctgat tgccgaagaa
    12001 gtcgacatta tcgaagtggg taccattctg tgcgtgggcg aaggcgtgcg tgcggttcgt
    12061 gacctgaaag cgctctaccc gcacaaaatc gtactggcag acgctaaaat tgctgatgcg
    12121 ggcaaaatcc tttcgcgtat gtgcttcgaa gccaacgctg actgggtgac ggtaatttgc
    12181 tgtgcggata tcaacaccgc caaaggcgcg ctggacgtag caaaagagtt taacggcgac
    12241 gtgcagatcg aattgaccgg ctactggacc tgggaacagg cgcagcagtg gcgtgacgca
    12301 ggcattcagc aggtggttta tcaccgcagc cgtgacgcgc aggccgcagg cgtagcgtgg
    12361 ggcgaagcgg acattaccgc gatcaaacgt ctttccgata tgggcttcaa agtcaccgtc
    12421 accggtggcc tggcgctgga agatctgccg ctgttcaaag gtattccgat tcatgtgttt
    12481 atcgccggtc gcagcatccg tgatgccgct tctccggttg aagccgcgcg tcagttcaaa
    12541 cgttccatcg ccgaactgtg gggctaagga gcggatatgt tgtccaaaca aatcccgctt
    12601 ggcatctatg aaaaagcgct ccccgccggg gagtgctggc tggaacgcct gcgactggca
    12661 aaaacgttgg gcttcgattt tgtcgagatg tcggtagatg aaactgacga gcgcctgtcg
    12721 cgcctcgact ggagccgcga gcagcgtctg gcgctggtga atgcgattgt tgaaaccggc
    12781 gtgcgcgtgc cgtccatgtg cctttctgct catcgtcgtt tcccgctggg cagtgaagat
    12841 gacgcggtgc gggcgcaggg gctggagatt atgcgtaaag ctatccagtt cgcccaggac
    12901 gtcggcattc gcgtgatcca gctggcgggc tatgacgttt actatcagga agccaataac
    12961 gaaacgcgtc gtcgtttccg tgacggcctg aaagagagcg ttgagatggc gagccgcgcg
    13021 caggtgacgc tggcgatgga gatcatggat tatccgttga tgaactccat cagcaaggcg
    13081 ctgggttacg cgcactatct caacaacccg tggttccagc tctacccgga tatcggcaac
    13141 ctgtcggcgt gggacaacga tgtgcagatg gagttgcagg ccggaatcgg gcatatcgtc
    13201 gcggtacatg tgaaagacac caaacctggc gtcttcaaaa acgtgccgtt tggcgaaggt
    13261 gtagtggatt tcgaacgttg tttcgaaacg ctcaaacaga gtggctattg cgggccgtac
    13321 ctgattgaga tgtggagcga aacggcggaa gacccggcgg cagaagtggc gaaagcgcgt
    13381 gattgggtga aagcgcgcat ggcgaaagcg ggcatggtgg aggcggcata atgcaaaagc
    13441 taaaacagca ggtatttgaa gccaacatgg atctgccgcg ctatgggctg gtgaccttta
    13501 cctggggcaa cgtcagcgct atcgaccgcg aacgcgggct ggtggtgatc aagcccagcg
    13561 gcgtcgccta cgaaaccatg aaagcagacg atatggtggt ggtcgatatg agcggtaagg
    13621 tggtggaagg ggaatatcgt ccgtcttccg acaccgcgac gcatcttgaa ctctaccgtc
    13681 gttatccgtc gcttggcggc attgtccata cccactccac tcatgctacc gcatgggcgc
    13741 aggcggggct ggcgatcccg gcgttaggca ctacgcatgc cgactacttc tttggcgaca
    13801 tcccgtgtac gcgtggatta agcgaagaag aggtgcaggg tgagtatgaa ctgaacactg
    13861 gcaaagtgat tatcgaaacg ctgggcaacg ccgagccgct gcatacgccg ggaattgtgg
    13921 tgtatcagca cggaccgttc gcctggggga aagatgccca cgacgcggtg cataacgcgg
    13981 tagtgatgga agaagtggcg aaaatggcgt ggattgcgcg cagtattaat ccacaactca
    14041 atcacatcgc cagcttcctg atgaataaac acttcatgcg taagcatggt cctaacgctt
    14101 attacgggca gaagtaaaac acgcgctgcg gaaatttcct tcttcgggag ataactggtc
    14161 taattccgca gccgtttttc aaaaaaagcc ccctgcgaag ggggcaaagc aaactatggc
    14221 aatgtttcgt tggttatacc tggtgctagc gataaatatc cgcgctggcg tgcatattgc
    14281 cgttactccc cggttcccgc atcagaatta cgtggtagta cgttgcgccc tgcgcatctg
    14341 tttcttcatt taatgcctga cgtgcttcac tttcagtggc gaaattatga ttgatataaa
    14401 tcacgcctaa gctttgcaca tcgtccatat ttctggcctg gtggttattg atttcaatgg
    14461 ctgcccatgt atttgcactt agcaaaagca cagccagaag ggctaaaaca cgactgaaca
    14521 tagatacctc ctcgacggct gactttgtgt gctctcctct gtgatgatct tctgatttaa
    14581 ttttaatcaa tgataaagaa gttgatggtg accatttctg atgcagttgt tcgaaaaaac
    14641 actgtgacgg aatgtgatga acttcaaatc agggagttag ttcttttgag cgaaagatga
    14701 gatttatttg tcggctggcc ttgagtttag cggggcatac aagtactata acgcgtcatt
    14761 tttcagccga cctttaacac gttccttgcc tccccgggat tcggctgacc cagacaggag
    14821 gctgaataat ccgtaaggag caattcgatg cgtcattacg aaatcgtttt tatggtccat
    14881 cctgaccaga gcgaacaggt tccgggcatg atcgagcgct acactgctgc catcactggt
    14941 gcagaaggca agatccaccg tctggaagac tggggccgcc gtcagctggc ttacccgatc
    15001 aacaaactgc acaaagcaca ctacgttctg atgaacgttg aagctccgca ggaagtgatc
    15061 gatgagctgg aaactacctt ccgcttcaac gatgccgtta tccgcagcat ggttatgcgt
    15121 accaagcacg ctgttaccga agcatctccg atggttaaag cgaaagacga gcgccgtgag
    15181 cgtcgcgatg atttcgcaaa cgaaaccgct gatgatgctg aagctgggga ttctgaagag
    15241 taatttctga tgaccaaccg tctggtgttg tccggcaccg tgtgcaggac tccccttcga
    15301 aaggtcagtc catcaggaat tcctcactgc cagttcgtgc ttgagcatcg ttctgtgcag
    15361 gaggaagccg gctttcaccg gcaggcatgg tgtcaaatgc ccgttattgt tagcggacac
    15421 gaaaaccagg ccattactca cagtataacg gtcggcagtc gcataaccgt tcaggggttc
    15481 atttcatgcc acaaggcaaa gaacggactg agcaaaatgg ttttgcatgc cgagcagatt
    15541 gaattgatag attctggaga ctagccatat ggcacgttat ttccgtcgtc gcaagttctg
    15601 ccgtttcacc gcggaaggcg ttcaagagat cgactataaa gatatcgcta cgctgaaaaa
    15661 ctacatcacc gaaagcggta agattgtccc aagccgtatc accggtaccc gtgcaaaata
    15721 ccagcgtcag ctggctcgcg ctatcaaacg cgctcgctac ctgtccctgc tgccgtacac
    15781 tgatcgccat cagtaatcgg tcacggtcca ttaatacgac tttgagagga taaggtaatg
    15841 caagttattc tgcttgataa agtagcaaac ctgggtagcc tgggtgatca ggtaaacgtt
    15901 aaagcgggct atgctcgtaa cttcctggta ccgcagggta aagctgttcc agctaccaag
    15961 aaaaacattg aattcttcga agcacgtcgc gctgaactgg aagctaaact ggctgaagtt
    16021 ctggcagctg ctaatgctcg cgctgagaaa atcaatgcac tggaaactgt taccatcgcg
    16081 tctaaagctg gcgacgaagg taaactgttc ggttccatcg gtactcgcga catcgctgac
    16141 gctgtaactg cagctggcgt tgaagtggct aagagcgaag ttcgtctgcc gaacggcgtt
    16201 ctgcgtacca ctggcgaaca cgaagtgagc ttccaggttc acagcgaagt attcgcgaaa
    16261 gtgatcgtaa acgtagtagc tgaataattc gttattcaac gagacgtaaa aaaacgccga
    16321 ccattggtcg gcgttttgct tttgtagcga gcacttgcag atatcgataa gggccacaat
    16381 aagcagccct acgaagataa ttacttagcc gcctgaatat cagccagcat tggggcaagg
    16441 gctgggttat ccttagcgaa gtttgcgtac attggctgta ctttctcgat aaacggagct
    16501 ttatctacct ggtagacttt cacgcccatt ttatccagtt ttgcaaattc ttgtttctct
    16561 tcctcgctcc acaagtcttt catgtagctc attgattctt tagctgcttt taatataact
    16621 tcctgatttt ccgggctaag tttatcaaac gcagcattgc tcatcagaac cacatcggga
    16681 accatagtgt gttcgtcaag gctgaaggat tttgccactt caccgtgacg catggtggtc
    16741 agcgccatca cgctgttttc tgccatatcg accacaccct gctgtaaggc tgtatacaac
    16801 tcgccgtaat ccagtggcgt cgggttaccg cccatgactt tgatcatttc aactgcgcca
    16861 gggcttggct ggacacggac tttcatgcct ttcagatcgt ctggtttcag tactggttta
    16921 ttgccataga aactgcgggc gcctccatca taaaaagtca gcccgaaata acctttgctt
    16981 ttcgttgagt cgaggatttt tctcccgata tcgccctgca tgacctgata ataatgatca
    17041 cgatcgcgga acaaataagg caggctaaat aatttatagg aattttcaaa tgcttccatt
    17101 tctgccgcat tggtttttac cagtggaata gcgccagaac gaatcagctc cattgtttct
    17161 cgttgagtac ccaatgttcc atttggataa atacgaatag taatatcacc gttagagtac
    17221 tctttgctct ttttcgcaaa gaactccatc gctttatgaa caggatgaga cttatcctga
    17281 ttatgactca gttttaatgt cactgcccag gactggcttg cggataacat aagaacagca
    17341 ccgataatgc cagcggatac ttttattttc atagatgttc cttttctaat aaatatggcg
    17401 gcaggtgccg ctagtgaatg cgcatcagta acccagtgct cttggcaatg ccatactgat
    17461 ttgcggaata taggtcacca ttgccagcac caataccatc acggcataga atggcagcat
    17521 aggtttgata attttattaa tcggtaattt agatacacta caaccgacaa ataacacact
    17581 gcctacaggc ggagtacaaa taccgataca catgttcagc accatgacaa taccaaagtg
    17641 aatcgggtcc atgcccagtt cggtgacaac cggtaaaaat atcggcgtaa aaatcagtac
    17701 cgccggtgtc atatccataa aagtgccgat aatcaacagg ataatattga taataagcag
    17761 gattattatt gggttgtcag aaatagtgtt aagcaaatcg agaatcagga agggaacgtc
    17821 agcgtttgac attgcccatg acattcccat cgaagaacca attaataaca agacaattgc
    17881 agtggtaata acggattcca gaaatatttt aggtaaatct ttaactgaga tttcacgata
    17941 aagaaccagt gcaaggaaaa gggtataaat tacagcaatt gcggaggctt cggttggagt
    18001 aaaaataccg ctta
//
LOCUS       DAZGAN020000096        17920 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGAN020000096 DAZGAN020000000
VERSION     DAZGAN020000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.096"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(458..553)
                     /gene="yncL"
                     /locus_tag="ACHKYO_RS18205"
                     /old_locus_tag="pgaptmp_003641"
     CDS             complement(458..553)
                     /gene="yncL"
                     /locus_tag="ACHKYO_RS18205"
                     /old_locus_tag="pgaptmp_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HGU4430416.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            748..921
                     /gene="ortT"
                     /locus_tag="ACHKYO_RS18210"
                     /old_locus_tag="pgaptmp_003642"
     CDS             748..921
                     /gene="ortT"
                     /locus_tag="ACHKYO_RS18210"
                     /old_locus_tag="pgaptmp_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HGU4430417.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1007..1240
                     /gene="ydcY"
                     /locus_tag="ACHKYO_RS18215"
                     /old_locus_tag="pgaptmp_003643"
     CDS             1007..1240
                     /gene="ydcY"
                     /locus_tag="ACHKYO_RS18215"
                     /old_locus_tag="pgaptmp_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HGU4430418.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1241..1690)
                     /gene="ydcZ"
                     /locus_tag="ACHKYO_RS18220"
                     /old_locus_tag="pgaptmp_003644"
     CDS             complement(1241..1690)
                     /gene="ydcZ"
                     /locus_tag="ACHKYO_RS18220"
                     /old_locus_tag="pgaptmp_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4430419.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1687..2205)
                     /gene="mddA"
                     /locus_tag="ACHKYO_RS18225"
                     /old_locus_tag="pgaptmp_003645"
     CDS             complement(1687..2205)
                     /gene="mddA"
                     /locus_tag="ACHKYO_RS18225"
                     /old_locus_tag="pgaptmp_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HGU4430420.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            2386..3423
                     /gene="curA"
                     /locus_tag="ACHKYO_RS18230"
                     /old_locus_tag="pgaptmp_003646"
     CDS             2386..3423
                     /gene="curA"
                     /locus_tag="ACHKYO_RS18230"
                     /old_locus_tag="pgaptmp_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HGU4430421.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            3621..4286
                     /gene="mcbR"
                     /locus_tag="ACHKYO_RS18235"
                     /old_locus_tag="pgaptmp_003647"
     CDS             3621..4286
                     /gene="mcbR"
                     /locus_tag="ACHKYO_RS18235"
                     /old_locus_tag="pgaptmp_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HGU4430422.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(4322..6424)
                     /gene="pqqU"
                     /locus_tag="ACHKYO_RS18240"
                     /old_locus_tag="pgaptmp_003648"
     CDS             complement(4322..6424)
                     /gene="pqqU"
                     /locus_tag="ACHKYO_RS18240"
                     /old_locus_tag="pgaptmp_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HGU4430423.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNLGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKQYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            6666..7727
                     /gene="yncE"
                     /locus_tag="ACHKYO_RS18245"
                     /old_locus_tag="pgaptmp_003649"
     CDS             6666..7727
                     /gene="yncE"
                     /locus_tag="ACHKYO_RS18245"
                     /old_locus_tag="pgaptmp_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HGU4430424.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(7842..9341)
                     /gene="ansP"
                     /locus_tag="ACHKYO_RS18250"
                     /old_locus_tag="pgaptmp_003650"
     CDS             complement(7842..9341)
                     /gene="ansP"
                     /locus_tag="ACHKYO_RS18250"
                     /old_locus_tag="pgaptmp_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HGU4430425.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVIFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            9608..10225
                     /gene="yncG"
                     /locus_tag="ACHKYO_RS18255"
                     /old_locus_tag="pgaptmp_003651"
     CDS             9608..10225
                     /gene="yncG"
                     /locus_tag="ACHKYO_RS18255"
                     /old_locus_tag="pgaptmp_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HGU4430426.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            10301..10513
                     /gene="yncH"
                     /locus_tag="ACHKYO_RS18260"
                     /old_locus_tag="pgaptmp_003652"
     CDS             10301..10513
                     /gene="yncH"
                     /locus_tag="ACHKYO_RS18260"
                     /old_locus_tag="pgaptmp_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HGU4430427.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININTNKNIRQVC"
     gene            11334..13442
                     /locus_tag="ACHKYO_RS18265"
                     /old_locus_tag="pgaptmp_003653"
     CDS             11334..13442
                     /locus_tag="ACHKYO_RS18265"
                     /old_locus_tag="pgaptmp_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU4430428.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
     gene            13509..17732
                     /locus_tag="ACHKYO_RS18270"
                     /old_locus_tag="pgaptmp_003654"
     CDS             13509..17732
                     /locus_tag="ACHKYO_RS18270"
                     /old_locus_tag="pgaptmp_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HGU4430429.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     PFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVYPDGLESRREYDEPGRLVSETSRSGE
                     TVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTA
                     VHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAW
                     GKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYH
                     YDLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGH
                     RVAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEW
                     LTYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQS
                     QHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDD
                     ERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLS
                     RKPEETWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQCRSLAEK
                     IQQEGSEDGHGVVFPAELVGLLDRLEGEIRANCVSSESRQWLAQCGLTVERLAAQIEP
                     VYLPERKIHLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPG
                     QQYDKESGLYYNRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIE
                     NMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAMRHCYLMCRM
                     TKTFGSTIADVIGKNHEAAGNRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKY
                     NTGQLFGLDGIKADNPIKAKQGSSDASNY"
     gene            17716..>17920
                     /gene="ydcD"
                     /locus_tag="ACHKYO_RS18275"
                     /old_locus_tag="pgaptmp_003655"
     CDS             17716..>17920
                     /gene="ydcD"
                     /locus_tag="ACHKYO_RS18275"
                     /old_locus_tag="pgaptmp_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdcD"
                     /protein_id="HGU4430430.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVFYGHKTPKSV
                     EIYLSEKNIIYKIINDQKISRGNG"
BASE COUNT         4667 a         4401 c         4780 g         4072 t
ORIGIN      
        1 ttcaagagag ggactataaa aaattcaatc tttctgaggt attcagaggc ctataattat
       61 ctgttttgca tgataaatgg gcaagataac cagggcgggg tttgcaattt caatggggca
      121 gcgtcatcaa ggaggttgca aacatcctgc catgcatata ctttacgcca tctttttata
      181 tttacgttat tccttgaaag tataaagcat ttatagactc ccgcgcgtat aaagtattca
      241 tgatagaaaa atgtgaaaac aagattccgt ttgaatcagt aagctgtttg tatagacgac
      301 agtaggggga tgttttctac tcatttcaat gtagatgcca gcaaaaagaa gtaaatatta
      361 aggataatgt tgtaaacctg gagagtatga gaagatcttg cgtactgtca gagagttgat
      421 ttctctgaca gtacgtgctg cgtgcaaaat cactacatca aataaaccag ccaaatctca
      481 tagagataag agtaaacaaa ccaacagcag caaagacatt tatcagtact acagttctac
      541 tggaaacatt catttttgcc accttttatc attgccccta tgtgtatagt acgggttaag
      601 aaaatccgca ataatactca ttctgaagat ggataagggc aagttgctgt ttgatgattt
      661 tttctcaaac tggtgaaaaa acttgatgca cgtcaaaaaa tgacgcatat ttgcgcgcgt
      721 tttattcatc tggctggacg cccgtacatg tctctctatc aacatatgct tgttttttat
      781 gcggttatgg cagcaatcgc atttcttatc acctggtttc tttctcacga taagaaacgc
      841 atccgtttct taagcgcctt tctggtgggg gcaacatggc cgatgagttt cccggtggcg
      901 ctgttgtttt cactgtttta aatcttactc actggcttga cggcaccacg cagacttata
      961 tcatttggat gaatcgataa atttcacaag aggttaagga gaaagtatgt cgcatctgga
     1021 tgaagtcatc gcgcgcgtgg atgccgcgat tgaagagagc gtcattgccc atatgaacga
     1081 attattaatc gccctgagcg atgacgcgga gttaagtcgg gaagatcgct acacccagca
     1141 gcagcgtttg cgcaccgcta ttgctcatca cggtcgcaag cataaagaag atatggaagc
     1201 gcgccacgaa cagttaacca aaggcggcac catcctctga ttaaaatgag cgtctggcta
     1261 ccaaccaggc gccaaccaca agtaaaattg cgccacaaat tgggccgaac aacgcccgta
     1321 gtggtacgcc atgactgcgg aagatatcca gcatcagacc gccaattagc tggctggcga
     1381 ccagtactgc aatagttgtc gccgctccca cattctgata tccgctgata ctggcgaaga
     1441 caaaaaacga tcccagcaag ccgggtataa gtgtccacca gcgcacgctg gacaccagtt
     1501 cgccaaaccc cgccatgcct tgtttaaacc ataaaataga aacaaagagc acaatcccca
     1561 ccagtgagtt cagcaacatg gcgatgagaa tggtagagga ggtctgggtg atgcgcacca
     1621 tcagcgtgtt ctggactacc agaccaattc cggcggcaat caaaaaagca agggtgagcg
     1681 actgattcat ccaatcgcgt ccggttcagt gcgttcgtcg agttgcaact gcataaatgt
     1741 cagatccagc cagcgcccaa atttagtgcc cacctgcggc atttgtgcgg tgacgacaaa
     1801 tcccagcgac tggtgcagat gcagcgaggc ctgattttgc gattcgatcc cggcgaccat
     1861 gacatgcttc ccgcaatccc gcgcttcatc aatcaatcgg cttaacaatt tacgccccag
     1921 acctttgccc tgatgatcgg gatggacata aaccgaatgt tccacggtat ggcgaaaacc
     1981 atcgaaacta cgccagtcgc caaacgaggc atatcccgtc actacgccat cttcttcact
     2041 caccagcacc ggataacctg ctatggtccg cgcttcaaac caggcaatgc ggttatctgc
     2101 atcaaccgtt tggtcattcc agatagccgc cgtatacaac acggcgtggt tataaatttc
     2161 cgcaatggca gcacaatcgg ctttgcgggc aaaacggatg gacatgtctg gcctcgataa
     2221 aataatgatg atgatgttat caatactatc gaaaacagcc cgaagcaacg gattcctcga
     2281 tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca atcccgtgag
     2341 cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg gcaacaaaag
     2401 cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt tccggagaat
     2461 ttccgtcttg aagaagatga tgtcgccaca cccggtgaag gacaggtgtt actgcgcaca
     2521 gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc atcttattca
     2581 ccgcctgttg atattggcgg cgtgatggtc ggcggcacgg tgagccgtgt tgtggagtcg
     2641 aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg gcaggactat
     2701 gacatatcca gtggtgatga tctggtgaaa cttggcgatc atccgcaaaa tccatcgtgg
     2761 tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct ggatatcggt
     2821 cagcctaaag agggcgaaac gttggtggta gctgcggcga caggtcctgt gggagcaacg
     2881 gtggggcaaa tcggcaaact taaaggttgc agggtggtgg gggttgccgg tggcgcggaa
     2941 aaatgccgcc atgctatcga ggtgctgggc ttcgatgttt gtcttgatca ccacgcggat
     3001 gattttgccg aacaactggt gaaagcgtgc ccaaaaggta ttgatatcta ttatgaaaac
     3061 gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc gcgcattccc
     3121 gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc ggatcgttta
     3181 cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt tattatcgct
     3241 caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg ggtgaaagag
     3301 gataaaatcc actaccacga agatattact gatggtttag agaatgcgcc acagacgttt
     3361 atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc gggtgatgat
     3421 taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct tattttcagc
     3481 tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg ggtggtttat
     3541 gattattcac tttaatacac caggtgaact ccttctgccg tgcagacagg gttggacaga
     3601 aaacacgcga aggaattaaa atgcctggaa tgggaaaaat gaaacatgtc agtttgactc
     3661 tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa cctggcgcac
     3721 gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct gtgcgtgaag
     3781 cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa gcatttacag
     3841 ttccggaagt ggggaaacgt caattagacg aaatcaatcg gatccgctac gaactggaat
     3901 taatggcagt tgctctggct gttgaaaacc tcaccccgca agaccttgcg gaactccagg
     3961 aattgctcga gaaattacag caggcgcaag aaaagggcga catggaacaa atcattaatg
     4021 taaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc ctgtgtgaga
     4081 tgattgagca actgtgggtc aggatggggc ctggtttaca ttatctctat gaagcgatta
     4141 atccagcgga attacgggag catatagaaa actatcatct gttactcgct gcgttaaaag
     4201 caaaagacaa agagggatgc agacattgtc ttgctgaaat tatgcaacaa aatattgcta
     4261 ttttatacca gcaatacaat cgttaaagta aatgcccggc aaataccggg catcaaggta
     4321 attactcaaa tctccacgca atattcatgc cgacaccata atttcgtccg ggcgaaggtt
     4381 cgtaatatcg cccgtttgac tcattgacaa tgacagaacc aacgtattgt ttatcgaata
     4441 aattatcgac acgaccaaat aaatccacag ttaaattgtg gtaattgtat ttatacccgg
     4501 tgaataagcc gacgagagta taagacggcg cttttgccgt attttcatca tctgccataa
     4561 tatcgcccat ataacgcgct tccgtgcctg cataccaacc atcttccggt acataaccta
     4621 tcgacgcaaa gcccatatta cgggcgatcc ctggcatccg attaccgtta caatcctgtt
     4681 cattgcaaac attgctgcga taggtcgcat caagccaggt ccaggacgcg tttacgcgaa
     4741 aatctcctgc gaaacgttga tcccatgcca gttcagcgcc ttgacgacgg gtctttccgg
     4801 catttttgta agtcgtacgc ccaccgctac tgctatcgac aacaatttca tcatcagtgt
     4861 cggtctgaaa caatgcgaga ctaagcagcc catcaccaat acgcgtttta ctgccgatct
     4921 caattgtatc gttggtggat ggttttaaac ctaagttcat accgctttgc ccatcagcac
     4981 gataagacag ctcattaatc gtcggcgttt caaaacctcg cccggctgcc agatagatat
     5041 tccaggcatc ggtcattgca tattttaacg aaccggcagg tagccattta tgataactgg
     5101 catcaccgct gtcatcgccg ttacccggag taacgtaatg gtcgttggaa tcaaaccaca
     5161 cggagctgta gcgcacgcca gcatccagcg acagtttttc gctcagctgc cactgcgtct
     5221 gtaaataggg atcgatgttc cacatcagat tgcgttcgtc gcgacgcaac tcaccttttt
     5281 gcccgtactc cggcatgccg ctattcaggc ggaagttatt gtagcccttg cggttttcac
     5341 tcatgttttc gtagttcagg ccggtagtga acgtgaccgg aacgcccagt tcaccacggt
     5401 gtgtccagcg gctgtctatt ccctggtaat ggcgttgcag ggtaatcacg ccgcccgcat
     5461 gtgacgggtt aagttgtggt gccatgggta ttgactggta ctgggtcgtt tctcgctctc
     5521 cggcatacat catcacactc atatcatccc gcgaactcag gctacgttca tagcgcaacc
     5581 cagcctgagt ttgcttgatg gtttttcgcg tgtcgtactg ttctgcacga ggcgcttgtt
     5641 gtggattcgc cttccattct gctttggtta gcccacctgg gtcatctgct ttgatatcca
     5701 cactattgaa aatcaggctt aatttgctgg cttcatcaat gcgtacgccc agtttggcat
     5761 tggctaaatt tttctgtgcg ccactatggt cacgatagcc gtgggtcgta aaacgcgtgg
     5821 ttgagacggt gtaatcaaca tcgccgggtt gtgtgccgtc tcccgttgcg cccgttgctt
     5881 tcagcccata gcgccagctg ccaaaactgc cgtagtaact actggcttca atggttggtg
     5941 gctgttgtcc ggtctgggtg gtgacattca ttacaccgcc agacgcgttg ccatacaggg
     6001 cagaaaaggg gccacgcagc acttccacat tttgcacact gcttaaatcg atgttggatg
     6061 tttgcccttg cccgtcgggc atggtggcgg gaataccgtc cacatacagg cgaataccgc
     6121 gaataccgta agtggagcgg gagccaaatc cgcgaatcga cagctgtaaa tcttgcgcat
     6181 agttctgccg gttttgtacc tgcaaaccag gcacgctggt cagtgattcg gacaagttaa
     6241 tgcgcggtgt tgccaggcgc atctcctcgc catccaccac gcttactgct gcaggggtat
     6301 ccagttctga aaccacctgc ggcgcggcac tgacaatcat ggtctgttca tcagcggcaa
     6361 aaacaacggg ggaaaggaca agcagtgcgg gcaaaacggt ctgtcgaacg aaaacaatct
     6421 tcatgaaaaa agccaggtta agaatgggaa aacgccgtca tggtaataaa attgtaaatt
     6481 tatggaaaac gaaacggcac gatacgttaa gtaattgaga aaattgtagt cgtaacggca
     6541 agaaatgctc cacatttgag aaaataatga ttaccattcc catttataac aagagcgtaa
     6601 cgatgattac gcttagcgaa gcattgtgaa gcagcaaaaa tatcggttca tcaaagggag
     6661 tcgtcatgca tttacgtcat ctgttttcat cgcgtctgcg tggttcatta ctgttaggtt
     6721 cattgcttgt tgcttcatca ttcagtacgc aggccgcaga agaaatgctg cgtaaagcgg
     6781 taggtaaagg tgcctacgaa atggcatata gccagcaaga aaacgcgctt tggctcgcca
     6841 cttcgcaaag ccgcaaactg gataaaggcg gtgttgttta tcgtcttgac ccggttactc
     6901 tggaagtgac ccaagcgatc cataacgatc tcaagccgtt tggtgccacc atcaataaca
     6961 cgactcagac gttgtggttt ggtaacaccg taaacagcgc ggtcacggcg atagatgcca
     7021 aaactggcga agtgaaaggc cgtctggtgc tggatgatcg taagcgcacg gaagaggtgc
     7081 gcccgctgca accgcgtgag ctggtagctg atgatgccac gaacaccgtt tacatcagtg
     7141 gtattggtaa agagagcgtg atttgggtcg ttgatggcga gaatatcaaa ctgaaaaccg
     7201 ccatccagaa caccggtaaa atgagtaccg gtctggcgct ggatagcaaa ggcaaacgtc
     7261 tttacaccac taacgctgac ggcgaattga ttaccatcga caccgccgac aataaaatcc
     7321 tcagccgtaa aaagctgctg gatgacggca aagagcactt ctttatcaac attagccttg
     7381 ataccgccag gcagcgtgca tttatcaccg attctaaagc cgcagaagtg ttagtggtcg
     7441 atacccgtaa tggcaatatt ctggcgaagg ttgcggcacc agaatcactg gctgtgctgt
     7501 ttaaccccgc gcgtaatgaa gcctacgtaa cgcatcgtca ggcaggtaaa gtcagtgtga
     7561 ttgacgcgaa aagctataaa gtggtgaaaa cgttcgatac gccgactcat ccaaacagcc
     7621 tggcgctgtc tgccgatggc aaaacgctgt atgtcagtgt gaaacaaaaa tccactaaac
     7681 agcaggaagc tacccagccg gacgatgtga ttcgtattgc gctgtaatag atgtggcgag
     7741 ggtgaaaacg gagagacttg cctgactttt atcacgccgc atgcggcaca ccttcgtcag
     7801 taatctaaat ttgcccggca cgtagccggg caattttgca attagctcgc cgtttcaggc
     7861 ttaaacacaa tttcctgttt ttcttcatct tcctcgacga ctggcgcagt gctgtgaatt
     7921 tcagcaacgc gtttgcgcac accaaaccag cctataacca gcagaatacc gataatcggc
     7981 agcgccgcga tagtataagt cccgttcggg taatcgaacg ccatcagcac caggacactc
     8041 agtaaaaaca gtaatgtcag ccaggaagtg aagggcgcgc caggcagttt aaaactgaca
     8101 tccgctgctt tgccttgttt aattgcttta cgcaggcgca tctggcacac gatgataaac
     8161 gcccatgaag cgatgattcc cagcgacgcg aagttcaaca caatctcaaa tacgcgcgac
     8221 ggcaccagat agttgaggaa tacgccgacg acatacacaa ctagtgtcgc cagaatcccg
     8281 gcatacggca catgctgacg actcattttc gccataaaac tcggtgcgga accgcccatc
     8341 gccattgagc gcagaatacg tccggtgcag tacagacctg agttcaggct ggagagggca
     8401 gcggtcagca ccacaatatt cataatgctg ccgatatatg gcacacccag tttagagaaa
     8461 aacgtcacga acggactttg ccccgcctga tacgcgctcc acggcaatag cataaccagc
     8521 aacaccacgg agccgacgta aaacaggcca atacgccaaa tcacactgtt aatggcttta
     8581 ggcaccatgg tctgcggatc tttacattct ccggcagctg tacccaccat ttcaatggag
     8641 gcaaaagcaa acactacgcc ctgaatcaac accagcgcag gcagcaaacc gtgggggaag
     8701 aagccgccat tatcggtgat taaatgaaag ccagtggtgt tgccatccag cggctgacca
     8761 ctaccgagga acactgtacc cacgaccaaa aagatcacaa tggcgagcac tttaataagc
     8821 gcaaaccaga actccatctc cgcaaaccat ttcacaccga tcatattcat ggtgccaacg
     8881 atggtaagtg cagcgagcgc aaagacccac tgcggcacgc cgccaaacgc accccagtaa
     8941 tgcatataca gagcgacggc ggtaatatca acaatccccg tcatcgccca gttgatgaag
     9001 tacatccagc cagcaacata agcggctttc tcacccaaaa actcacgggc ataagagaca
     9061 aaactgccac tggaagggcg gtgtagcacc agctcaccca atgcacgcag aataaaaaac
     9121 gaaaacaagc cacaaattaa ataaaccagt gccagtgctg gacccgccat ttgcagtcgg
     9181 gctcctgcac ctaaaaacaa gccggtgcca atcgcgccgc caatggcgat catctgtacc
     9241 tggcgattgc ccatcgcttt gtgatacccc tcttcgtggg cattaagcca gcggcgtttc
     9301 gcggcgtgtt gatctgaagt gtcggtgtcg tgtttactca ttgctctccc tgattgcttt
     9361 aatgaaaaag tcatataagt tgccatgaac aatgtttatt catcctggcg atgatagtta
     9421 tcaccattat tctttatggg tataaaacta tcggcgcagc atcctacccg ttatcgataa
     9481 acgatgcaaa acatcccctt acaatcctga aggggattaa tacaactgac gaaaaaatga
     9541 caaatccttt tgctggttaa cctgtgtact gtcctacact taatctttaa aagattgtga
     9601 ggggcatatg attaaggtgt atggcgtacc cggatggggc tctacaatca gtgagctgat
     9661 gctaacactg gctgatattc cttatcagtt cgttgatgtc agtggttttg accacgaggg
     9721 aacttcacgc gagttattga aaaccctgaa cccgttgtgt cagattccga cactggcgct
     9781 ggaaaatgac gagattatga cggagacagc ggcgattgca ttgatggttc tcgatcgtcg
     9841 cccggacctt gcgccaccgg ttgggcgcgc cgaacgtcag cagtttcaac ggctattggt
     9901 ctggctggtt gctaatgtct atccaacatt cactttcgcc gattaccctg agcgttgggc
     9961 tcctgacgca cctgaacagt taaagaagaa tgtaattgaa tatcgtaaat cgctttatat
    10021 ctggctgaat tcacaattga ccgctgaacc ttatgcgttt ggtgagcaac taacgctggt
    10081 ggactgttat ctttgcacta tgcgcacatg ggggcctggg catgaatggt ttcaggataa
    10141 tgccacgaat atcagtgcga ttgctgatgc tgtatgtcag ttaccgaaat tacaagaagt
    10201 attaaaaagg aatgaaatta tttgagtggg ataccagagg attatgttat ttattattca
    10261 gtaactttat ccacaatgat gggcgtaatt aattaaattc atgttatgtt ttttgattta
    10321 tatcactctc cccttcattc agcttgtcta tttcatttcc tctgaaaaga aactaactat
    10381 tcacattgtg cagatgtttc acttgttatc acaggtattc tataatctca aaaagttttt
    10441 aatgatggat atgctcggag ttggagatgc aattaatatt aatacaaata aaaatatccg
    10501 gcaggtatgc taaaaggata aatggcgttc tctgtattta ttcgcaatta tctgtaggac
    10561 aaatatggag agcttgatga gtcagagcta aaacagcaat tgataaataa acatgttgat
    10621 ttattcaaga aaatggatgt attttaaata ttgattctta atataaccta atcaatagat
    10681 tatcacgtga gagctgcaca ttattggcgg ggccggggaa aacatcattt tgggaacaat
    10741 gaatcaggta ctcttaccac tgaagcaaga tcatccgggc acctggtggt ggtgcagtgc
    10801 tttggaaatg gtcccctcgg gggaattgtc attattttct atgctatttg cttatgatat
    10861 tcatcaggtg aatttaataa attcctaatt tgaaatatcc actattaagc cagtgtttaa
    10921 cgacgttaag atggcaatgt gcagatcatg ttatcagggt ggggcggtag cgtatattcc
    10981 taatacaaat catcattcat ttcaatgatt gttattgaat tatttattgt tgtgaataaa
    11041 aagacattat gaatattggt ttgactttgc atatcaggtt ttttattact cgctgtaatg
    11101 tacgagcctg tcgtgaataa cgtcgagaac ccatccgttg cgtttgtgat tattttgttg
    11161 actaaacaga cacccgtttc tctgaagtaa aatcccagac taaatcatca cataaccatg
    11221 acatttttct gatattcccc ggtaacgcca gatgtcgact cgcttatctc atccgcccga
    11281 catgttgtac atgcaatatg tgcggattat tattcatttt cacggaggtt gctatgtcaa
    11341 ccggattacg tttcacactt gaagtggacg gcctgccacc ggacgcgttt gcggtggttt
    11401 cctttcatct cacccagtca ctctcttcac ttttttccct cgacctttct ctggtcagtc
    11461 agcagtttct ttcccttgaa tttgcgcagg tgctggacaa aatggcctac ctgacggtat
    11521 ggcagggcga tgacgtacag cgccgggtga aaggtgtggt gacctggttt gaactggggg
    11581 agaacgacaa aaaccagatg ctgtacagca tgaaggtgtg cccgccgctg tggcgcacag
    11641 ggctgcgcca gaacttccgt atcttccaga atgaggacat cgaaagcatc ctcgctacga
    11701 tcctgaagga aaacggtgtg accgagtgga gtccgctgtt cagcgagccg catccttccc
    11761 gtgagttttg tgtccagtac ggcgaaaccg attacgattt cctgtgccgg atggcggcgg
    11821 aggaaggcat cttcttttat gaggagcacg cgcaaaaaag taccgaccag agcctggtcc
    11881 tgtgcgacac cgtgcgttat ctgccggagt cctttgagat cccctggaat ccgaacaccc
    11941 gtaccgaggt gagcaccctc tgcatcagcc agttccgcta cagtgcacaa atccgccctt
    12001 cttccgtggt gaccaaagac tacaccttta aacgacccgg ctgggcaggg cgttttgatc
    12061 aggaaggcca gtaccaggat taccagcgca cgcagtatga agtgtatgac taccccggac
    12121 gtttcaaggg tgcccacggg cagaactttg cccgctggca gatggacggc tggcgaaaca
    12181 atgcagaaac cgcgcgggga atgagtcgct cgccggagat atggccggga cgacgaattg
    12241 tgctgacggg gcatccgcag gcgaacctga accgggaatg gcaggtggtg gcaagtgaac
    12301 tgcacggcga acagccacag gcggtgccag gacggcaggg agcggggacg gcgctggaga
    12361 accattttgc ggtgatcccg gcagacagaa catggcgacc acagccgttg ctgaaaccgc
    12421 tggtcgacgg cccgcagagc gctgtcgtga caggaccggc aggcgaggaa atcttctgcg
    12481 acgaacatgg tcgcgtgcgg gtgaagttca actgggaccg ttataacccg gcagaccaag
    12541 acagttcgtg ctggatccgt gtggcacagg cgtgggcagg caccggtttt ggccacctgg
    12601 cgataccgcg tgtgggtcag gaggtgattg tggacttcct caacggcgat ccggaccagc
    12661 cgattattat ggggcgtacc taccaccagg aaaaccgcac acccggcagc ctgccgggga
    12721 cgaagacgca gatgaccatt cgttcgaaaa cctataaggg cagcgggttt aatgaactga
    12781 agtttgacga tgcgacaggg aaagaacagg tctacatcca cgcgcagaag aacatgaaca
    12841 ccgaggtgct gaataaccgc accacggatg tgataaacaa ccatgctgag accattggca
    12901 acaatcagat gattgcggtt accaacaatc agatacagac ggtgggcgtt aaccagatag
    12961 agacggtggg cagtaaccag atcattaacg tgggttctgt tcaggtggaa acgattggac
    13021 ttgttcgtgc gctgaccgtg ggcgtggcgt accagacgac ggtaggtggc attatgaaca
    13081 cttcggtggc tctgatgcag tcctcgcaga tcggtttgca taaatcgttg agggtcgggc
    13141 tgggttatga cgtcaaagtc ggaaataacg ttaccttcac cgttggtaaa acgaaaaagg
    13201 atgataccgg gcagaccgcg atttactccg ccggtgagca tctggagctc tgctgtggta
    13261 aggcaaggct ggtgctgacg aaggacggac aaatttttct caacggcaca aaaattcatt
    13321 tgcagggtaa ggagcaggtt aatggtgact cactgttgat taactggaac tgtgcagcct
    13381 cgaaatcccc accgaagacc cctgatgaaa agcaggatac gccggatatg agagagtact
    13441 gatcccgtac ctgttcggta ttcagtaaca aacaagcatt aaaaataaac agaagaagga
    13501 attgaatcat gagcggaaaa ccagcggcgc gtcagggaga tatgactcag tatggcggtc
    13561 ctattgtcca gggttcggca ggtgtaagaa ttggcgcgcc caccggcgtg gcgtgctcgg
    13621 tgtgtccggg cgggatgact tcgggcaacc cggtaaatcc gctgctgggg gcgaaggtgc
    13681 tgcccggcga gacggacctt gcgctgcccg gcccgctgcc gttcattctc tcccgcacct
    13741 acagcagcta ccggaccagg acacccgcgc cggtgggagt gtttggcccc ggctggaaag
    13801 cgccttctga tatccgctta cagctacgcg atgatgcact ggtactcaac gacaacggcg
    13861 ggcggagcat tcactttgag ccgctgctgc cgggggaggc ggtgtacagc cgcagcgagt
    13921 caatgtggct ggtgcgcggt ggtaaggcag cgcagccgga cggccacacg ctggcgcggc
    13981 tgtggggggc gctgccgccg gatatccggt taagcccgca tctttacctg gcgaccaaca
    14041 gcgcacaggg gccgtggtgg atactggggt ggtcagagcg ggtgccgggt gctgaggacg
    14101 tactgccagc gccgctgccg ccgtaccggg tgcttaccgg gctggcagac cgtttcgggc
    14161 ggacgctgac gtaccggcgt gaggccgcag gcgacctggc cggggaaatc accggcgtga
    14221 cggacggtgc cgggcgtgag ttccgtctgg tactgaccac acaggcacag cgggcggaag
    14281 aggcccgcac ctcttcgcta tcttcttctg acagttcccg ccctctctca gcctcaccgt
    14341 tccccgacac actgcccggt accgaatacg gccccgacag gggcatccgt ctttcggcgg
    14401 tgtggctgat gcacgacccg gcatacccgg aaagcctgcc cggtgcgcca ctggcgcggt
    14461 acacgtatac ggaagccggt gaactgctgg cggtatatga ccgcagcaat acgcaggtgc
    14521 gcgctttcac gtatgacgcg cagcacccgg gccggatggt ggcgcaccgt tacgcgggaa
    14581 ggccggagat gcgctaccgc tacgacgata ccgggcgggt ggtggagcag ctgaacccgg
    14641 caggcctgag ttaccgctac cagtatgagc aggaccgcat caccgtcacc gacagcctga
    14701 accggcgtga ggtgctgcat acagaaggcg gggccgggct gaagcgggtg gtgaaaaaag
    14761 aactggcgga cggcagcgtc acgcacagcg ggtatgacgc ggcaggaagg ctcacggcgc
    14821 agacggacgc ggcgggacgg cggacagagt acggtctgaa tgtggtgtcc ggcgatatca
    14881 cggacatcac cacaccggac gggcgggaga cgaaatttta ctataacgac gggaaccagc
    14941 tgacggcggt ggtgtacccg gacgggctgg agagccgccg ggaatatgat gaaccgggca
    15001 ggctggtatc ggagacatcg cgcagcgggg agacagtacg ctaccgctac gatgacgcgc
    15061 acagtgagtt accggcgacg acaacggatg cgacgggcag cacccggcag atgacgtgga
    15121 gccgctacgg gcagttgctg gcgttcactg actgctcggg ctaccagacc cgctatgaat
    15181 acgaccgctt cggccagatg acggcggttc accgcgagga aggtatcagc ctttaccgcc
    15241 actatgacaa ccgtggccgg ttaacctcgg tgaaagacgc acagggccgt gaaacgcagt
    15301 atgagtacaa cgccgcaggc gacctgactg ccgttatcac cccggacggc aaccggagcg
    15361 agacacagta cgatgcgtgg ggaaaggcgg tcagcaccac gcagggcggg ctgacgcgca
    15421 gtatggagta cgatgctgcc ggacgtgtca tcagtctgac taacgagaac ggcagccaca
    15481 gcgacttcag ctacgatgcg ctggaccggt tggtacagca gggcggcttt gacgggcgga
    15541 cgcaacgtta tcattatgac ctgaccggaa aactcacgca gagtgaagat gaggggcttg
    15601 tcaccctctg gtactacgat gaatcggacc gtatcactca ccgcacggtg aacggcgaac
    15661 cggcagagca gtggcagtat gatgaccacg gctggctgac agacatcagc cacctgagcg
    15721 aaggccaccg tgtcgctgtc cactacggct acgacgataa gggccgcctg accggcgaac
    15781 ggcagacggt ggagaacccg gagacggggg aactgctgtg gcagcatgag acaacacacg
    15841 cctacaacga acaggggctg gcaaaccgcg tcacgccgga cagcctgccg ccggtggagt
    15901 ggctgaccta cggcagcggt tatctggcgg gcatgaagct tggcgacacg ccgctgctgg
    15961 agtacacgcg cgaccgcatg caccgggaga cggtgcgcag ctttggcagc atggcaggca
    16021 gtaatgccgc atataaactg accagcacat acacccccgc aggccagtta cagagccagc
    16081 acctgaacag cttggtgtat gaccgtgact acgggtggaa tgacaacggc gacctggtgc
    16141 gcatcagcgg cccgcgacag acgcgggaat acggctacag cgccacgggc aggctggaga
    16201 gcgtgcgtac cctcgcaccg gacctggaca tccgcatccc gtatgccacc gacccggcgg
    16261 gaaaccgcct gccggacccg gagctgcacc cggacagtac cctcacggcg tggccggata
    16321 accgcatcgc agaggatgcg cactatgttt accactatga tgaatacggc agactgacgg
    16381 agaagacgga ccttatcccg gcgggtgtta tccggacgga cgacgagcgg acccaccact
    16441 accactacga cagccagcac cgtctggtgt tctacacgcg gatacagcat ggcgagccac
    16501 tggtcgagag ccgctacctc tacgacccgc tgggacggcg aatggcgaaa cgggtatggc
    16561 ggcgggagcg tgacctgacg gggtggatgt cgctgtcgcg taaaccggag gagacatggt
    16621 acggctggga cggcgacagg ctgacgacgg tacagaccga caccacgcgt atccagacgg
    16681 tataccagcc ggggagcttc gcgccgctca tccgcatcga aacagacaac ggtgaacggg
    16741 agaaagcgca gtgccgcagc ctggcggaga agatacagca ggaagggagc gaagacggtc
    16801 acggtgtggt gttcccggct gaactggtgg ggctgctgga caggctggag ggggaaatcc
    16861 gggcaaactg cgtgagcagt gaaagccgcc agtggcttgc gcagtgcggg ctgacggtgg
    16921 agcggctggc ggcacagata gagccggttt atttgcctga gcgaaaaata catctttatc
    16981 actgtgacca ccggggcctg ccgctggcgc ttatcagtga agacggcaat acggcgtgga
    17041 gcgcggaata tgatgaatgg ggcaaccagc ttaatgagga gaacccgcat cacctgcacc
    17101 agccgtaccg gctgccgggg cagcagtatg ataaggagtc ggggctgtac tataaccgtc
    17161 accggtacta cgatccgttg caggggcggt atatcacccc ggacccgatt gggttgagag
    17221 gtggatggaa tatgtatcag tatccgttga atcccataca agtgatagac ccaatggggt
    17281 tagatgcgat tgagaatatg acatcaggtg gactaattta tgccgtatct ggtgtacctg
    17341 gattgattgc tgcaaacagc attactaaca gtgcttacca gttcggttat gatatggatg
    17401 ctattgttgg cggagctcat aatggggccg ccgatgcaat gagacattgt tacttgatgt
    17461 gtcgaatgac taagacattt ggatcaacaa tagctgacgt gataggtaaa aatcatgagg
    17521 cggcagggaa tagacaaggt cagccagcta aagaaagaat catggatctt aaaaataaca
    17581 ctgtcggtat tgcttgtggc gatttttctg ccaaatgtag cgatgcatgt attgaaaaat
    17641 ataacactgg gcaactcttc gggttagatg gtataaaagc agataatcca ataaaagcaa
    17701 agcaagggag ttcagatgct tcaaattatt agaggcaaac ttgtcatttt tttaattacc
    17761 ctttgtttat ttgttgttta ccttgggttt gataacaatt caaattctga catcgtattt
    17821 tatggacata aaacaccaaa gagcgttgag atatatcttt ctgaaaaaaa tattatttat
    17881 aaaataatta atgaccaaaa aattagtaga gggaatggtc
//
LOCUS       DAZGAN020000097        17771 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGAN020000097 DAZGAN020000000
VERSION     DAZGAN020000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.097"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            189..917
                     /locus_tag="ACHKYO_RS18280"
                     /old_locus_tag="pgaptmp_003656"
     CDS             189..917
                     /locus_tag="ACHKYO_RS18280"
                     /old_locus_tag="pgaptmp_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430431.1"
                     /translation="MNNSKLKKILIASIIGNILLVFLNIQVFNKKSAQDITETDKVGK
                     TEIKKTKSTWDDINTVNPICNVNYTTDSPTFYDKEKSRSEPFCFWFNPATDKYTFLKN
                     TLISSRGDGMFFFSKEHTPIITLYPPETGISVPIDIYFKQRLDRLSHGLNAKIIQPKE
                     AKLIKSKNEEKLISSVVVVPTDKSRREYPREGFPLLVVIIKKNGTLWKVSVDYIDDYL
                     LNDKKHWYNDKEIFDLIDNITSTF"
     gene            complement(937..1311)
                     /locus_tag="ACHKYO_RS18285"
                     /old_locus_tag="pgaptmp_003657"
     CDS             complement(937..1311)
                     /locus_tag="ACHKYO_RS18285"
                     /old_locus_tag="pgaptmp_003657"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4430432.1"
                     /translation="MKKFPLAWYTDVLFNNYFNYSGRASRREFWWYYLFNLIFLGVLF
                     FIGYTLDNTGISYGFYIPCVYVLATIIPSITLKVRRLHDIGLSAWYLFVWCIPYIGGF
                     ILFVMYCIPSEKGDNKYGAYSI"
     gene            1658..2293
                     /locus_tag="ACHKYO_RS18290"
                     /old_locus_tag="pgaptmp_003658"
     CDS             1658..2293
                     /locus_tag="ACHKYO_RS18290"
                     /old_locus_tag="pgaptmp_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HGU4430433.1"
                     /translation="MTSQLIPVFNGSISNQAALLCNARNLHAFLGVGKRFASWITERI
                     AEYGFVENQDYMIASQIREAKGRGGHNRKDYHLTLDTAKELAMVERNEKGRQIRRYFI
                     ECEKKFRQSLLPAPMNINYPLSWFSEHHPYSMMSYVDRKTLNLDVSVLFDMPSPTMRI
                     LNELHSKGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
     gene            2349..2780
                     /locus_tag="ACHKYO_RS18295"
                     /old_locus_tag="pgaptmp_003659"
     CDS             2349..2780
                     /locus_tag="ACHKYO_RS18295"
                     /old_locus_tag="pgaptmp_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HGU4430434.1"
                     /translation="MKQIAFYRRSGRPGAFRGLKERVTWMIQSRGRPVTGSEIAEKFG
                     VSLCEFNKVARGLTKGSKVVKIKASEPFTTDTGIVDRFFSLESNPRRDTPRSRNAVPP
                     FSRRSREHAAKNCREEYVQKAERRRRLIKAGLYIDEFENAL"
     gene            2777..3403
                     /locus_tag="ACHKYO_RS18300"
                     /old_locus_tag="pgaptmp_003660"
     CDS             2777..3403
                     /locus_tag="ACHKYO_RS18300"
                     /old_locus_tag="pgaptmp_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HGU4430435.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            3363..3575
                     /locus_tag="ACHKYO_RS18305"
                     /old_locus_tag="pgaptmp_003661"
     CDS             3363..3575
                     /locus_tag="ACHKYO_RS18305"
                     /old_locus_tag="pgaptmp_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU4430436.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADAELH
                     LFGEILSRKLDELEKLVEEADTSPTV"
     gene            3611..4000
                     /locus_tag="ACHKYO_RS18310"
                     /old_locus_tag="pgaptmp_003662"
     CDS             3611..4000
                     /locus_tag="ACHKYO_RS18310"
                     /old_locus_tag="pgaptmp_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4430437.1"
                     /translation="MNAVLTELNKLGKASAESISKGLNIDLNDVIDTLWKLKNQGVVT
                     VKNGIWQAVAREVDKKPNIASVQPVQPVQPVQPVQPVQHNIIGDLLRKSRKEARRAGR
                     KQKRWEGACKALQELNKYRDLINELSE"
     gene            4144..4377
                     /locus_tag="ACHKYO_RS18315"
                     /old_locus_tag="pgaptmp_003663"
     CDS             4144..4377
                     /locus_tag="ACHKYO_RS18315"
                     /old_locus_tag="pgaptmp_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430438.1"
                     /translation="MKITFESYGLTASVVISSSIFESRKHQHIVDAVKLKAPEVTVTT
                     HGLFRIRTTLTTTHLSAFRVYDMALKEYNQCSS"
     gene            4365..4616
                     /locus_tag="ACHKYO_RS18320"
                     /old_locus_tag="pgaptmp_003664"
     CDS             4365..4616
                     /locus_tag="ACHKYO_RS18320"
                     /old_locus_tag="pgaptmp_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4430439.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIG
                     AFNEEFERIEHNELHMIKAEIEKEKHIASLRKMRRTSIN"
     gene            4677..4859
                     /locus_tag="ACHKYO_RS18325"
                     /old_locus_tag="pgaptmp_003665"
     CDS             4677..4859
                     /locus_tag="ACHKYO_RS18325"
                     /old_locus_tag="pgaptmp_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4430440.1"
                     /translation="MEINLMTEKEVSELLQKRRTALYNLRKKHGFPDPVLTHPARYSR
                     QAVEQWLKAGGINRAV"
     gene            complement(4843..6012)
                     /locus_tag="ACHKYO_RS18330"
                     /old_locus_tag="pgaptmp_003666"
     CDS             complement(4843..6012)
                     /locus_tag="ACHKYO_RS18330"
                     /old_locus_tag="pgaptmp_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4430441.1"
                     /translation="MALSDTKLRGLYGKPYSGPAEITDGDGLSVRITPAGTITFQYRY
                     RWNGKPVRLTVGRYPSTSLKDARVIVGEMRALYMKGVNPKNYFAPSDGELTLKECLDQ
                     WWDKYVSGLKPNTQTLYRSVVYNTMYTQFEDSPVAGIPVSAWARFFDKQESLNKKKAR
                     VLLLQLRSVINWCISRQLIPSCELLKLSVKNIGKKPDVGSRVLTYTELAKIWLALENS
                     KVVTSNKLLHQLLLLWGARLSELRLATASEFNMEDLVWTTPKEHSKMGNIIRRPIFTQ
                     VEPYIERLLNIGFDVLFPGQEIDKPIDRSSANLYMKKLRAKIDIPEWRTHDFRRSLVT
                     NLSGEGIMPHVTEKMLGHELGGVMAVYNKHDWLSEQKDAYELYADKIFWHAKQLG"
     gene            6208..6282
                     /locus_tag="ACHKYO_RS18335"
                     /old_locus_tag="pgaptmp_003667"
     tRNA            6208..6282
                     /locus_tag="ACHKYO_RS18335"
                     /old_locus_tag="pgaptmp_003667"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6241..6243,aa:Arg,seq:cct)
     gene            complement(6724..7971)
                     /locus_tag="ACHKYO_RS18340"
                     /old_locus_tag="pgaptmp_003668"
     CDS             complement(6724..7971)
                     /locus_tag="ACHKYO_RS18340"
                     /old_locus_tag="pgaptmp_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HGU4430442.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYSGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(8043..8957)
                     /locus_tag="ACHKYO_RS18345"
                     /old_locus_tag="pgaptmp_003669"
     CDS             complement(8043..8957)
                     /locus_tag="ACHKYO_RS18345"
                     /old_locus_tag="pgaptmp_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HGU4430443.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            9173..10606
                     /gene="cscA"
                     /locus_tag="ACHKYO_RS18350"
                     /old_locus_tag="pgaptmp_003670"
     CDS             9173..10606
                     /gene="cscA"
                     /locus_tag="ACHKYO_RS18350"
                     /old_locus_tag="pgaptmp_003670"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HGU4430444.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(10614..11609)
                     /locus_tag="ACHKYO_RS18355"
                     /old_locus_tag="pgaptmp_003671"
     CDS             complement(10614..11609)
                     /locus_tag="ACHKYO_RS18355"
                     /old_locus_tag="pgaptmp_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4430445.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKPGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            11852..12070
                     /locus_tag="ACHKYO_RS18360"
                     /old_locus_tag="pgaptmp_003672"
                     /pseudo
     CDS             11852..12070
                     /locus_tag="ACHKYO_RS18360"
                     /old_locus_tag="pgaptmp_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            12088..13416
                     /gene="dsdA"
                     /locus_tag="ACHKYO_RS18365"
                     /old_locus_tag="pgaptmp_003673"
     CDS             12088..13416
                     /gene="dsdA"
                     /locus_tag="ACHKYO_RS18365"
                     /old_locus_tag="pgaptmp_003673"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HGU4430446.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(13524..15062)
                     /gene="emrY"
                     /locus_tag="ACHKYO_RS18370"
                     /old_locus_tag="pgaptmp_003674"
     CDS             complement(13524..15062)
                     /gene="emrY"
                     /locus_tag="ACHKYO_RS18370"
                     /old_locus_tag="pgaptmp_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HGU4430447.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAHIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(15062..16225)
                     /gene="emrK"
                     /locus_tag="ACHKYO_RS18375"
                     /old_locus_tag="pgaptmp_003675"
     CDS             complement(15062..16225)
                     /gene="emrK"
                     /locus_tag="ACHKYO_RS18375"
                     /old_locus_tag="pgaptmp_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HGU4430448.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALIMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            16641..17255
                     /gene="evgA"
                     /locus_tag="ACHKYO_RS18380"
                     /old_locus_tag="pgaptmp_003676"
     CDS             16641..17255
                     /gene="evgA"
                     /locus_tag="ACHKYO_RS18380"
                     /old_locus_tag="pgaptmp_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HGU4430449.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            17260..>17771
                     /locus_tag="ACHKYO_RS18385"
                     /old_locus_tag="pgaptmp_003677"
     CDS             17260..>17771
                     /locus_tag="ACHKYO_RS18385"
                     /old_locus_tag="pgaptmp_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4430450.1"
                     /translation="MKFLPYFFLLCCDLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVA"
BASE COUNT         5291 a         4118 c         3834 g         4528 t
ORIGIN      
        1 tttgatggtg aagagattaa aaacttaatg agcctagaaa tacaaaaaaa attaagctct
       61 ggcgagtgta aaatgatgca ggagtataat tatatataca aatatcatta caacataata
      121 aacacgaaac ataatgactt agttataatt atgacaaaga gtaattgccc tcaatgaagg
      181 atgagtatat gaacaactca aaactgaaga aaatacttat agcatccatt atcgggaaca
      241 tacttttagt ttttttaaac attcaagtat tcaataaaaa aagtgcccag gacatcacgg
      301 aaacagacaa ggtaggcaaa actgaaataa aaaaaacaaa aagcacttgg gatgacataa
      361 ataccgttaa ccctatatgt aatgttaact atactactga cagtcccact ttctatgaca
      421 aggaaaagtc acgttcagag ccattttgtt tctggtttaa ccccgcaaca gataaatata
      481 catttctcaa gaatactcta atatcatcac gcggtgatgg tatgtttttc ttttcgaaag
      541 aacatacacc aatcataaca ttataccctc cagaaactgg aataagtgtt cctattgata
      601 tatattttaa acaacgtctg gatcgcctaa gtcatggact gaatgccaaa attatccagc
      661 ctaaagaggc aaaactgata aaaagtaaaa atgaagagaa actgatttca tcagttgttg
      721 tcgttcctac agataaatca agaagagaat acccaagaga aggattccct ttacttgtag
      781 ttattattaa aaaaaacggg acattatgga aagtttcagt tgattatata gatgattatc
      841 tacttaatga taaaaagcac tggtacaatg ataaagaaat atttgattta atagataata
      901 taaccagtac tttttgatgc gattatctgg cggggattaa atactatatg caccgtattt
      961 attatcccct ttttctgacg ggatacagta cataacgaac aatataaagc caccaatata
     1021 cggaatgcac cagacaaaca agtaccaggc acttaaccca atatcatgga gtctgcggac
     1081 tttcaatgta atggatggaa taatcgtagc taatacgtaa acgcaaggta tataaaaccc
     1141 ataactaatg ccagtattat ctaatgtata tccgataaaa aaaagaactc ccaagaatat
     1201 taaattaaac agataatacc accagaactc tcttcgactg gctcgccctg aataattgaa
     1261 gtagttatta aaaagaacat ccgtatacca tgccaaaggg aattttttca tgcttttata
     1321 ctccaaacat taatattaaa ttgataatta ttaacaactt aaaacaacgt taatgtttta
     1381 cctaataggg tgtcaacatc tacacaagga aatgttgaca cgccacacat tctgtcggat
     1441 actcctcttc atggtgctga acacaccaca gcaagcggaa accgccccgt cagtcatgcg
     1501 gttttttttg tgtccatttt tcagatatgg tcgggtagcg cgtataccga aaaacagccg
     1561 taaggctaag gatacgggcc gacttgctgc ggtgttcaag tacctgacca ccctgctgaa
     1621 cacagggtta tctgaacaaa tcagcaagga cataaaaatg actagtcaac tcatccccgt
     1681 attcaacggt tctatctcaa accaagcagc ccttctatgc aatgctcgca atctacacgc
     1741 tttccttggg gttggtaaac gctttgcatc gtggataaca gaacgtattg ctgaatatgg
     1801 tttcgttgaa aatcaggact atatgattgc ttcccaaatt cgggaagcaa aaggtagagg
     1861 cggtcacaac cgcaaggact accacctcac cctggacaca gccaaagagc ttgcgatggt
     1921 tgaacgtaac gaaaaaggtc gccagatccg ccgctacttc atcgagtgcg agaaaaagtt
     1981 tcgccagagc cttttacctg caccaatgaa catcaactac cctctctcgt ggttttcaga
     2041 gcatcacccc tactccatga tgagctatgt tgatcgaaaa actcttaacc tggacgtttc
     2101 tgtgctcttc gatatgccaa gcccaactat gcgcatcctc aacgagctac acagcaaggg
     2161 ctataacgtt gacgccgctg tcgctgaatt taacgccttc aaacatctga cggaagaaat
     2221 gcgccgcacg ctacaggata tttcaagact gtcagatcga aattcccgaa aaggcttctc
     2281 gttaagcctg taacttcccc taccatcccc gacatcccgt cggggttttc atatctggag
     2341 acaaaaattt gaaacagatc gctttctaca ggcgttctgg aaggcccggt gccttccgtg
     2401 ggttaaaaga acgcgtcacc tggatgattc agtctcgtgg tcgtcctgtt actggcagtg
     2461 aaatagcaga gaaatttggt gtttcacttt gcgagttcaa caaggtagct cgcggcctga
     2521 caaagggcag taaggttgtg aaaataaagg cttcagaacc attcaccaca gacaccggaa
     2581 tcgttgatcg ctttttctcc ctcgaatcaa atcctcgtcg tgacacacct cgctcacgca
     2641 atgccgttcc tccattcagt cgcagaagcc gtgaacacgc agcaaaaaac tgtcgcgagg
     2701 aatacgtgca aaaggccgaa cgccgtcgcc gactgattaa agcaggactt tacattgatg
     2761 agtttgaaaa cgcgctatga cgaaaaaata caccctcatt tacgcagatc ccccctggac
     2821 attccgcgac aaagcaaccg atggtcaacg cggtgcaagt tttaaatatc cggtcatgag
     2881 tcttctggat atctgccgcc tcccggtatg ggaactggca gccgataact gcctgttggc
     2941 tatgtggtgg gtgcctacac aaccacttga agcattgaag gttgtagaag catggggctt
     3001 tcgcctggtg acgatgaaag gattaacctg gaacaaatgc gggaaaagac agaccgacaa
     3061 gctggtcatg ggtatgggta gcaccactcg tgctaacagc gaagactgcc tttttgcagt
     3121 gaaaggaaat ctgcctgaac gcattaacgc cggaataatt cagtcattta ctgcaccacg
     3181 ccttgatcac tcccgcaagc cggatatggc gcgagaaaaa cttgtgcaac ttcttggcga
     3241 tgttccccgg atagaactgt tcgcccgcca cacctcgcat ggatttgatg tatggggtaa
     3301 ccaatgcggc acaccatcca ttgagatggt tccgggtatt gttaaatttc tggagaaaac
     3361 caatgagcga aaaaacgacg ttgacaaagg catcaccagt tgaattaagg cagtgtctgg
     3421 aaatcgcaaa tcaacttgcc agaagtggaa tacgatttgt tccaatcccg attacagcag
     3481 atgcagaact tcatctgttt ggtgaaattc tttcccgaaa gctggatgaa ctggaaaagc
     3541 tggtagaaga agctgacacc tcaccaaccg tataacagcc ccaccgacat taaaatatca
     3601 ggagaaaaaa atgaacgcag tgctcacaga attgaacaaa ttaggaaaag catcagccga
     3661 aagtatttct aaaggtctca atattgattt gaatgacgtt attgacactc tatggaagtt
     3721 aaaaaaccag ggggtagtaa ctgtaaaaaa tggcatctgg caggcagttg caagggaagt
     3781 ggacaaaaaa ccaaatatcg cctcagtgca gccagtgcag ccagtgcagc cagtgcagcc
     3841 agtgcagcca gtgcagcaca acattatagg tgacctgcta cgtaaatcac ggaaagaagc
     3901 gcgtcgcgcc gggcggaaac agaaacgatg ggagggtgca tgtaaggcgc tgcaagaact
     3961 gaataaatac cgtgacttga tcaacgaatt gtcagagtga gcgaatcaca gagagaatat
     4021 tttgagaaac aacccgcgta cattaatatt gtttttcctt tctctaatga cgggctgtat
     4081 cctgttattc gcaggaacaa cattatttat ttttgttgcc cggctaactg cgaggataat
     4141 gacatgaaaa tcacatttga aagttacggt ttaacagcaa gtgtggttat ctccagctct
     4201 atattcgaaa gccgcaaaca tcagcatatc gttgatgcag tgaaactcaa agccccggaa
     4261 gttaccgtca caacacatgg acttttcagg atacgaacga cgttgactac aacacacctc
     4321 tcggcattcc gtgtttatga tatggcactc aaggaataca accagtgttc gtcctgatcc
     4381 agcgcgggca gtctttcgtt gatgccaaca actatccggt agaaatatgc aaggtaactc
     4441 tgactcaggt gatctaccga aggctcgacg gcagaaccag agccacttca attggtgcat
     4501 ttaatgaaga atttgagcga atagagcaca acgaactgca tatgattaaa gcggaaattg
     4561 agaaggaaaa gcatattgcc agccttcgaa aaatgcgccg tacatcaatc aactaacaac
     4621 cgccttcggg cggtttatta tgctgttgaa aaacatgtag aaaaggagag catactatgg
     4681 aaatcaactt aatgaccgaa aaagaagttt ctgagttgtt acaaaaaagg cgcacagctt
     4741 tgtataattt gcgaaaaaaa cacggattcc ctgatccagt acttactcat ccggcgcgat
     4801 atagccgtca ggccgttgaa caatggctta aagccggagg aattaaccga gctgtttagc
     4861 gtgccagaaa attttatcag catacaactc atacgcatct ttctgttccg acaaccagtc
     4921 gtgtttatta tacacggcca tcactcctcc cagttcatgc cccagcattt tttcagtgac
     4981 gtggggcata attccttccc cagataaatt cgttaccaga gaacgcctaa aatcatgcgt
     5041 tctccattca ggaatatcaa ttttcgccct gagttttttc atgtacaaat tagcagacga
     5101 acggtcaata ggtttatcta tttcctgccc agggaacagc acatcaaatc cgatattaag
     5161 caatcgctca atataaggct caacctgagt aaatatcgga cgtcggataa tattacccat
     5221 ctttgaatgc tctttagggg ttgtccagac cagatcttcc atgttgaact cactggctgt
     5281 agcgagacgt agctcagaaa gcctggcccc ccatagcaat agcaattgat gtaacagctt
     5341 gttggaagta accacttttg agttctccag tgccagccag atctttgcca gttccgtata
     5401 ggtaagtacg cgactcccca catctggttt cttcccaata ttctttacac tgagtttgag
     5461 taattcgcat gatgggatta actggcggct gatacaccag ttaataactg aacgcagttg
     5521 taacaacaga acacgagcct ttttcttgtt gaggctttct tgtttgtcaa aaaagcgagc
     5581 ccaggccgat acagggatac cggcaactgg cgagtcttca aactgtgtgt acatggtgtt
     5641 gtacacaaca gatctataaa gcgtctgagt attgggtttc aggccgctaa catacttatc
     5701 ccaccactga tccaagcatt cttttaatgt caattcgcca tcactggggg caaaataatt
     5761 tttagggtta actcccttca tgtacaatgc gcgcatctca ccgacgataa cacgtgcgtc
     5821 cttcagtgaa gtggacggat agcgcccgac agtaagacgt actggcttac cattccagcg
     5881 ataacggtac tgaaacgtga tagttcctgc tggagtaatc cgcacactta acccgtcacc
     5941 atcggtgatt tcagcagggc cagagtaggg ttttccgtat aggcctcgta gtttggtgtc
     6001 actcagtgcc aatgctttat atcctgtaca caagtttata atgtattctg tactcaatat
     6061 gtacgcatta gcaagtgaac aaaacacttt tcaacgcaaa agaagataat caaccatgaa
     6121 caaaaacgaa cttacacctt gattttaaaa ccataaagac ggtattatca gtcatatggt
     6181 gaacaaccaa aatcaatacg caacaacgtc ctcttagtta aatggatata acgagcccct
     6241 cctaagggct aattgcaggt tcgattcctg caggggacac cagataccct tcaaacgaaa
     6301 tctaccttca ccccgtaaaa gatgggtttg gcagcacact tgccttatat ctactcattt
     6361 ttactgcaac aggttgaaat ctcagcactg tcagaaagcg ctgatgacta aacagccctg
     6421 agccgggcga tgtaaccatc acacagaatc ctgatagcga aatatggcgt gactcgatac
     6481 ttcactccgc aatgcattcc ttgatgaatt cgcaggaccg tgatacacgg gacaggtcac
     6541 tgaatgacga caatgtcctg gaaatcagcg aaccgcgcat ctgaagtaca tttgagcgac
     6601 tgtaccagaa catgaatgag gcgtttggat taggcgatta ttagcagggc taagcatttt
     6661 actattatta ttttccggtt gagggatata gagctatcga caacaaccgg aaaaagttta
     6721 cgtctatatt gctgaaggta caggcgtttc cataactatt tgctcgcgtt ttttactcaa
     6781 gaagaaaatg ccaaatagca acatcaggca gacaataccc gaaattgcga agaaaactgt
     6841 ttggtagcct gcgtggtcaa agagtatccc agtcggcgtt gaaagcagca caatcccaag
     6901 cgaactggca atttgaaaac caatcagaaa gatcgtcgac gacaggcgct tatcaaagtt
     6961 tgccacgctg tatttgaaga cggatatgac acaaagtgga acctcaatgg catgtaacaa
     7021 cttcactaat gaaataatcc aggggttaac gaacagcgcg caggaaagga tacgcaacgc
     7081 cataatcaca actccgataa gtaatgcatt ttttggccct acccgattca caaagaaagg
     7141 aataatcgcc atgcacagcg cttcgagtac cacctggaat gagttgagat aaccatacag
     7201 gcgcgttcct acatcgtgtg attcgaataa acctgaataa aagacaggaa aaagttgttg
     7261 atcaaaaatg ttatagaaag accacgtccc cacaataaat atgacgaaaa cccagaagtt
     7321 tcgatccttg aaaactgcga taaaatcctc tttttttacc cctcccgcat ctgccgctac
     7381 gcactggtga tccttatctt taaaacgcat gttgatcatc ataaatacag cgccaaatag
     7441 cgagaccaac cagaagttga tatggggact gatactaaaa aatatgccgg caaagaacgc
     7501 gccaatagca tagccaaaag atccccaggc gcgcgctgtt ccatattcga aatgaaaatt
     7561 tcgcgccatt ttttcggtga agctatcaag caaaccgcat cccgccagat accccaagcc
     7621 aaaaaatagc gcccccagaa ttagacctac agaaaaattg ctttgcagta acggttcata
     7681 aacgtaaatc ataaacggtc cggtcaagac caggatgaaa ctcatacacc agatgagcgg
     7741 tttcttcaga ccgagtttat cctgaacgat gccgtagaac atcataaata gaatgctggt
     7801 aaactggttg accgaataaa gtgtacctaa ttccgtccct gtcaacccta gatgtccttt
     7861 cagccaaata gcgtataacg accaccacag cgaccaggaa ataaaaaaga gaaatgagta
     7921 actggatgca aaacgatagt acgcatttct gaatggaata ttcagtgcca taattacctg
     7981 cctgtcgtta aaaaattcac gtcctattta gagataagag cgacttcgcc gtttacttct
     8041 cactattcca gttcttgtcg acatggcagc gctgtcattg cccctttcgc cgttactgca
     8101 agcgctccgc aacgttgagc gagatcgata attcgtcgca tttctctctc atctgtagat
     8161 aatcccgtag aggacagacc tgtgagtaac ccggcaacga acgcatctcc cgcccccgtg
     8221 ctatcgacac aattcacaga cattccagca aaatggtgaa cttgtcctcg ataacagacc
     8281 accacacctt ctgcaccttt agtcaccaac agcatggcga tctcatactc ttttgccagg
     8341 gcgcatatat cccgatcgtt ctgtgttttt ccactgataa gtcgccattc ttcttccgag
     8401 agcttgacga catccgccag ttgtagcgcc tgccgcaaac acaagcggag caaatgctcg
     8461 tcttgccata gatcttcacg aatattagga tcgaagctga caaaacctcc ggcatgccgg
     8521 atcgccgtca tcgcagtaaa tgcgctggta cgcgaaggct cggcagacaa cgcaattgaa
     8581 cagagatgta accattcgcc atgtcgccag cagggcaagt ctgtcgtctc taaaaaaaga
     8641 tcggcactgg ggcggaccat aaacgtaaat gaacgttccc cttgatcgtt cagatcgaca
     8701 agcaccgtgg atgtccggtg ccattcatct tgcttcagat acgtgatatc gactccctca
     8761 gttagcagcg ttctttgcat taacgcacca aaaggatcat cccccacccg acctataaac
     8821 ccacttgttc cgcctaatct ggcgattccc accgcaacgt tagctggcgc gccgccagga
     8881 caaggcagta ggcgcccgtc tgattctggc aagagatcta cgaccgcatc ccctaaaacc
     8941 catactttgg ctgacatttt tttcccttaa attcatctga gttacgcata gtgataaacc
     9001 tctttttcgc aaaatcgtca tggatttact aaaacatgca tattcgatca caaaacgtca
     9061 tagttaacgt taacatttgt gatattcatc gcatttatga aagtaaggga ctttattttt
     9121 ataaaagtta acgttaacaa ttcaccaaat ttgcttaacc aggatgatta aaatgacgca
     9181 atctcgattg catgcggcgc aaaacgccct agcaaaactt catgagcacc ggggtaacac
     9241 tttctatccc cattttcacc tcgcgcctcc tgccgggtgg atgaacgatc caaacggcct
     9301 gatctggttt aacgatcgtt atcacgcgtt ttatcaacat catccgatga gcgaacactg
     9361 ggggccaatg cactggggac atgccaccag cgacgatatg atccactggc agcatgagcc
     9421 tattgcgcta gcgccaggag acgataatga caaagacggg tgtttttcag gtagtgctgt
     9481 cgatgacaat ggtgtcctct cacttatcta caccggacac gtctggctcg atggtgcagg
     9541 taatgacgat gcaattcgcg aagtacaatg tctggctacc agtcgggatg gtattcattt
     9601 cgagaaacag ggtgtgatcc tcactccacc agaaggaatc atgcacttcc gcgatcctaa
     9661 agtgtggcgt gaagccgaca catggtggat ggtagtcggg gcgaaagatc caggcaacac
     9721 ggggcagatc ctgctttatc gcggcagttc attgcgtgaa tggaccttcg atcgcgtact
     9781 ggcccacgct gatgcgggtg aaagctatat gtgggaatgt ccggactttt tcagccttgg
     9841 cgatcagcat tatctgatgt tttccccgca gggaatgaat gccgagggat acagttaccg
     9901 aaatcgcttt caaagtggcg taatacccgg aatgtggtcg ccaggacgac tttttgcaca
     9961 atccgggcat tttactgaac ttgataacgg gcatgacttt tatgcaccac aaagcttttt
    10021 agcgaaggat ggtcggcgta ttgttatcgg ctggatggat atgtgggaat cgccaatgcc
    10081 ctcaaaacgt gaaggatggg caggctgcat gacgctggcg cgcgagctat cagagagcaa
    10141 tggcaaactt ctacaacgcc cggttcacga agctgagtcg ttacgccagc agcatcaatc
    10201 tgtctctccc cgcacaatca gcaataaata tgttttgcag gaaaacgcgc aagcagttga
    10261 gattcagttg cagtgggcgc tgaagaacag tgatgccgaa cattacggat tacagctcgg
    10321 cactggaatg cggctgtaca ttgataacca atctgagcga cttgttttgt ggcggtatta
    10381 cccacacgag aatttagacg gctaccgtag tattcccctc ccgcagcgtg acacgctcgc
    10441 cctaaggata tttatcgata catcatccgt ggaagtattt attaacgacg gggaagcggt
    10501 gatgagtagt cgaatctatc cgcagccaga agaacgggaa ctgtcgcttt atgcctccca
    10561 cggagtggct gtgctgcaac atggagcact ctggctactg ggttaacata atatcaggtg
    10621 gaacaacgga tcaacagcgg gcaagggatc cgcgtcactc ttcccccttc acgaccttca
    10681 ataatatgca atgcagcttc ccgcccgata atgtcatgtg gaagctgaat tgtggtcagc
    10741 ggcggtaaaa acagatgccc gacgccaacc agattatcaa agcccattac ggcgacatcc
    10801 tgcgggatac gtaccccctt cgccaggaga acctgataag cgacaaaggc tgcgcgatcg
    10861 ttaccacata tcagaacatc aaaatctggt ttgcccggtt tgaagtgggc attgagtaaa
    10921 cttgcgagat cggtgtagtg atcatcacct gttgccatgt gaaattgttt cacctcagcc
    10981 agatctcgtc cagcatcacg ccaggcctgc tcaaatccct gccgacgata ccctgttgcc
    11041 aacgcacttt ccggtagcca gaagcataac ggttgacgat agcccgccgc gagcaaatgc
    11101 tgtgttgatt catattgtgc agtgtaatca tcagggatat aactgggtaa cgctgggtca
    11161 tccgccacac agttcgccaa tacaatattt tcaccataca gagactcagg cagcgtgata
    11221 tgtcgcagcc ccattgtagt atagataatg ccatccggac ggtgggcaag cagctgacgt
    11281 gccgcgcggg cagcgtcatc ttcagaaaaa atattgatta aaaaactatt ccagccgaac
    11341 tcgctggcgg tttgctcaat ggcaagcaga atatcaacag agaaaggagt ggtagccgtg
    11401 tcctgcgcca gcacggcgag agtcgacggc ttacgtcctt gagcgcgcat cttacgggcg
    11461 gaaagatcag gaacataatt cagggtctgg attgcctgca atacgcggtc acgcgttgca
    11521 ggacgcacag attctgcatt atgcatcacc cgggagactg tcatcatcga cactcccgcc
    11581 aggcgtgcga catcctttaa tgaagccata cccaagccgt ttgccgtaaa acgggcactg
    11641 tagcagaaac agacgtcact ggcgagatcc aacgatattc tcaacgccct atcacccaac
    11701 acggcaatac aataaaaaat aacaataatt gccggataat tgtactcaat tccgcctctg
    11761 ttctcgccaa cgagtaacca tataggcaag taagtaaaac atgccaactg gcatcgccaa
    11821 cctggagaag aaaatataaa tatggatcat tgtgctgccg ctgtatcccg acatcagccc
    11881 ggaaattatt gcgattgcta tcggttcagg tgcaattggc tgcacgatcg ttacggactc
    11941 gcttttctgg ctagtgaagc aatattgcgg cgctacgctc aatgaaacat ttaaatacta
    12001 tacgacagcg acatttatcg cttcagtcgt cgctctggcg ggcacattcc tgctgtcatt
    12061 tatcatctaa gcgcaaagag acgtactatg gaaaacgcta aaatgaactc gctcatcgcc
    12121 cagtatccgt tggtaaagga tctggttgct cttaaagaaa ccacctggtt taatcctggc
    12181 acgacctcat tggctgaagg tttaccttat gttggcctga ccgaacagga tgttcaggac
    12241 gcccatgcgc gcttatcccg ttttgcaccc tatctggcaa aagcatttcc tgaaactgct
    12301 gccactgggg ggattattga atcagaactg gttgccattc cagctatgca aaaacggctg
    12361 gaaaaagaat atcagcaacc gatcagcggg caactgttac tgaaaaaaga tagccatttg
    12421 cccatttccg gctccataaa agcacgcggc gggatttatg aagtcctggc acacgcagaa
    12481 aaactggctc tggaagcggg gttgctgacg cttgatgatg actacagcaa actgctttct
    12541 ccggagttta aacagttctt tagccaatac agcattgctg tgggctcaac cggaaatctg
    12601 gggttatcaa tcggcattat gagcgcccgc attggcttta aggtgacagt tcatatgtct
    12661 gctgatgccc gggcatggaa aaaagcgaaa ctgcgcagcc atggcgttac ggtcgtggaa
    12721 tatgagcaag attatggtgt tgccgtcgag gaaggacgta aagcagcgca gtctgacccg
    12781 aactgtttct ttattgatga cgaaaattcc cgcacgttgt tccttgggta ttccgtcgct
    12841 ggccagcgtc ttaaagcgca atttgcccag caaggccgta tcgtcgatgc tgataaccct
    12901 ctgtttgtct atctgccgtg tggtgttggc ggtggtcctg gtggcgtcgc attcgggctt
    12961 aaactggcgt ttggcgatca tgttcactgc ttttttgccg aaccaacgca ctccccttgt
    13021 atgttgttag gcgtccatac aggattacac gatcagattt ctgttcagga tattggtatc
    13081 gacaacctta ccgcagcgga tggccttgca gttggtcgcg catcaggctt tgtcgggcgg
    13141 gcaatggagc gtctgctgga tggcttctat acccttagcg atcaaaccat gtatgacatg
    13201 cttggctggc tggcgcagga agaaggtatt cgtcttgaac cttcggcact ggcgggtatg
    13261 gccggacctc agcgcgtgtg tgcatcagta agttaccaac agatgcacgg tttcagcgca
    13321 gaacaactgc gtaataccac tcatctggtg tgggcgacgg gaggtggaat ggtgccggaa
    13381 gaagagatga atcaatatct ggcaaaaggc cgttaataac gtttcaacgc agcatcgcaa
    13441 tcctttccct gggtgagcga tgctgccgat ggcgcagact taagatcccc ggtcttaccc
    13501 gctataaccc cctcctttta tcatcaccca acgcctttcg ctgtaaacgg cggtttcgca
    13561 aaccaaacca aaaccgtaag taagataaaa gcaatagccg ccatacggaa aatctcattt
    13621 gcagaaatag aaagtgactg ctgggttatt tcattattaa tttcattaag aactcctgaa
    13681 agcgaaccat agtatttatc cataatttgt gacgatgaat taaacacggg gttaaattga
    13741 tcgatggttg ctgtcaactg actatggtgt aacgattcgc gtcgtcccca cagcgtcatt
    13801 gtcaacgacg taccaactga tcctgacaag gtacgaaaaa aattactcat actcgaggca
    13861 ttggcaaatt tattatctgg caagcctgaa aacgaaatcg ttgttaaggg taaaaagaaa
    13921 caggcaacgg cgaatccctg gaaaaactgt ggcaaaatga tgcctgtaaa atcaatcgtt
    13981 ggcataaatg tcacagaacg ccagtaatag caaaccgcat acatcaaaaa actgaatgtc
    14041 actaacaccc gcatgtctat tttattgcca taacgtccta tcaaaggtga aatcaatagt
    14101 ggcatgatgc cgatgtgcgc ataagcaagc ccggcccata tcgcattata ccccatcgtt
    14161 tcctggagta actgcggcat aaggacgatc gctccagagt aaaataaata cgcgcaggtg
    14221 atactcacaa taccaatggt gaaattacga gacttaaaca aactgagatc aagaatcgga
    14281 ttctctgagg tcgactccca aatgactaaa gagatcagag aaataactga tactactgtt
    14341 aatattatta ttgtactcga gttgaaccaa tccagatcgc gccctttatc aagcataatt
    14401 tgcaagccac caacaccgag cactaacagg gtcagtcctg gtagattcat ttttaccggt
    14461 gaagtttcag tttctcttcc tttaagtaag gttaagcata atgtcaggac gataatcccc
    14521 atagggacat tgattaaaaa tatccaaccc cagctaaagt tatcgcaaat ataaccgccc
    14581 aatatcggcc cacatatcgg agcgataatc acggtcattg accataatgc cagagcaaat
    14641 gttctttttt ctggtggata attcctcaat aataaactct gtgacagtgg aattaacggc
    14701 cccgccatta atccctgaac gactctaaaa aatatcagca catcaagatt ggtcgataaa
    14761 ctacacatta aggaagacag cgaaaaaaaa gtgactgaaa gtaaaaataa ccttaattcg
    14821 cctattcttt gtgccagcct gccggtaaca gggatcgcaa tggcatttgc tacgccaaac
    14881 gaggtgataa cccaggtgcc ttcgtctgtt gatgctccca gaaagccaga tattgtcggt
    14941 attgcgacgt tagaaatagt ggaatccaac atttgcataa atgtcgctaa tgacaatgca
    15001 atagtgacgc accataacgt cccaccggtt aatggtgccg gagttgattt agtgattgcc
    15061 attaaagttg tccattatgc gaaataatgt tgctaatttc tttttctatc ggactggtat
    15121 cgataactaa agccttactg gtataagccg gcatggaggt cacggttgaa gccagctcag
    15181 gcatctcggc aatgtcttcg ttcttcgtat caatagttgc tgtcatcgat aaaccaatac
    15241 gcaagggatg ttccatgagt tcttttggat caagagaaac ttcaaccggt acacgctgaa
    15301 cgattttgat ccagttccct gtcgcatttt gtgcaggtaa taaggagaac gcattgccgg
    15361 ttcccatatt gatccctgtc acccgaccat gaaacacaac attttcacca taaagatcgc
    15421 tgataatatt gaccgattga ccaatccgta catccgtaag ttgtgtttct ttaaagttgg
    15481 cattaaccca catttgacgt gccggtacga cagccattaa cgattgtccg gggctcactg
    15541 tttcgccgac ctgaacactt ctctgggcaa tatagccggt aaccggactc ttaatatccg
    15601 tacgtttaag cgccaaccag gcttctttag ttgcatccgc cgcttcaacg acttgtggct
    15661 gacggtttaa tggtgtgttc attattaaag ctttattcgc tttataagcc tggatagcgg
    15721 cattcaatgc cgctttgcta cttattaacg tatctttggt atgctccagc gtttcttttg
    15781 aaataacccc ctgcttcgct aacggcactc gacggttata atcttctaaa gattgttgat
    15841 actgaatacg tgctgaagcg acttcggcac tgtattgttt atcctgtaag tatagtttat
    15901 tcgtttgccg aacaatattt gccagattat ttttagcttt attgagtgcg atagtggcat
    15961 cagttttatc cagtgaaact aaaatgtcac cttgtcgaac gtagttcgta tctttatgat
    16021 taacgacagt gacactacct gagacttgtg cagaaattgg atctgcattc cccgtgacat
    16081 aggcgtcatc tgtactaatc atgtcttcta attccattga ccaataggca taggcacctg
    16141 aaaacgcaat aaataaaact accgccaata aagaaaagta ttttcttctg ttagaatgtt
    16201 ttttatttga attaatctgt tccactatta tctctcattt ctcatagatg aaatttatga
    16261 attgaatgaa aagagcattc attcttcata tcaaacacag tgcatgtgtt gcacattatc
    16321 ccaggaacgt tggaggccta atttaaatga ggtgcaaaaa aaacaacaga ggactaaacc
    16381 gtggcttttg caatacaatt cttacgcctg taggattagt aagaagactt atagtgccaa
    16441 cttgaaacta taaatcatcg gtacaatccc tgattttatt gttgacattt catttatgcc
    16501 gactatttat atggtatact tgtcgaatta tcttaaagga agctcagatt ttcttatttt
    16561 tattgagaaa atgagatgac gccttatgtc tgtattacta cagggagaag ggagatgctt
    16621 cattgcaaag ggaataatct atgaacgcaa taattattga tgaccatcct cttgctatcg
    16681 cagcaattcg taatttattg atcaaaaacg atattgaaat cttagcggag ttgactgaag
    16741 ggggaagcgc cgttcagcgg gtggaaacac ttaagcctga tatcgtcatc attgatgtcg
    16801 atatccccgg agttaacggt atccaggtgt tagaaacgct gaggaagcgc caatatagcg
    16861 gaattattat tattgtctcc gctaaaaatg accattttta cgggaaacat tgtgctgatg
    16921 ctggcgctaa tggattcgtg agtaaaaaag aaggcatgaa caatatcatt gcggctattg
    16981 aagctgcaaa aaatggctac tgctatttcc ccttctctct caaccggttt gttggaagtt
    17041 taacgtccga ccagcaaaaa ctcgactcct tatcgaaaca agaaattagt gtcatgcggt
    17101 atattcttga tggcaaggat aataatgaca ttgctgaaaa aatgttcatc agcaacaaaa
    17161 ctgtcagcac ttataaaagt cgcctgatgg aaaaattaga atgtaaatca ctgatggatc
    17221 tttacacatt cgcgcaacgt aacaaaatcg gctaatcaca tgaagttttt accctatttt
    17281 tttcttctct gttgtgatct ttggtcgacc ataagtttcg cagacgaaga ttacatcgaa
    17341 tatcgtggca tcagtagtaa caaccgtgtc acacttgatc cagtacgtct gagcaacaag
    17401 gaattacgtt ggttagcgag caaaaaaaat cttgtgattg cagtacacaa gtcccaaacg
    17461 gctaccttgt tgcataccga ttcgcagcaa caggttcgtg gtattaatgc tgattattta
    17521 aatcttttaa aaagagcgtt aaatatcaaa ttaacactcc gggaatacgc agatcatcaa
    17581 aaagcaatgg acgcgctgga agaaggtgaa gtcgatatag tgttatcaca tttagttact
    17641 tcgccgcctc ttaataatga cattgctgca accaaaccat tgataattac ctttccggcg
    17701 ctggtaacca cccttcacga ctcaatgcga ccgcttacct caccaaaacc agtaaatatt
    17761 gctcgggtag c
//
LOCUS       DAZGAN020000098        17742 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZGAN020000098 DAZGAN020000000
VERSION     DAZGAN020000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.098"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            156..866
                     /locus_tag="ACHKYO_RS18390"
                     /old_locus_tag="pgaptmp_003678"
     CDS             156..866
                     /locus_tag="ACHKYO_RS18390"
                     /old_locus_tag="pgaptmp_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4430451.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            908..3559
                     /locus_tag="ACHKYO_RS18395"
                     /old_locus_tag="pgaptmp_003679"
     CDS             908..3559
                     /locus_tag="ACHKYO_RS18395"
                     /old_locus_tag="pgaptmp_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4430452.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYYSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            3573..4103
                     /gene="ydeS"
                     /locus_tag="ACHKYO_RS18400"
                     /old_locus_tag="pgaptmp_003680"
     CDS             3573..4103
                     /gene="ydeS"
                     /locus_tag="ACHKYO_RS18400"
                     /old_locus_tag="pgaptmp_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4430453.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAEMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4116..4619
                     /gene="ydeR"
                     /locus_tag="ACHKYO_RS18405"
                     /old_locus_tag="pgaptmp_003681"
     CDS             4116..4619
                     /gene="ydeR"
                     /locus_tag="ACHKYO_RS18405"
                     /old_locus_tag="pgaptmp_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4430454.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4678..5592
                     /gene="ydeQ"
                     /locus_tag="ACHKYO_RS18410"
                     /old_locus_tag="pgaptmp_003682"
     CDS             4678..5592
                     /gene="ydeQ"
                     /locus_tag="ACHKYO_RS18410"
                     /old_locus_tag="pgaptmp_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4430455.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            5913..6038
                     /locus_tag="ACHKYO_RS18415"
                     /old_locus_tag="pgaptmp_003683"
                     /pseudo
     CDS             5913..6038
                     /locus_tag="ACHKYO_RS18415"
                     /old_locus_tag="pgaptmp_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6042..6620
                     /gene="ydeO"
                     /locus_tag="ACHKYO_RS18420"
                     /old_locus_tag="pgaptmp_003684"
                     /pseudo
     CDS             6042..6620
                     /gene="ydeO"
                     /locus_tag="ACHKYO_RS18420"
                     /old_locus_tag="pgaptmp_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
     gene            7022..8704
                     /gene="ydeN"
                     /locus_tag="ACHKYO_RS18425"
                     /old_locus_tag="pgaptmp_003685"
     CDS             7022..8704
                     /gene="ydeN"
                     /locus_tag="ACHKYO_RS18425"
                     /old_locus_tag="pgaptmp_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4430456.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            8756..9913
                     /gene="ydeM"
                     /locus_tag="ACHKYO_RS18430"
                     /old_locus_tag="pgaptmp_003686"
     CDS             8756..9913
                     /gene="ydeM"
                     /locus_tag="ACHKYO_RS18430"
                     /old_locus_tag="pgaptmp_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU4430457.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQCYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(9879..10001)
                     /locus_tag="ACHKYO_RS18435"
                     /old_locus_tag="pgaptmp_003687"
     CDS             complement(9879..10001)
                     /locus_tag="ACHKYO_RS18435"
                     /old_locus_tag="pgaptmp_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430458.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            10204..11889
                     /gene="yddA"
                     /locus_tag="ACHKYO_RS18440"
                     /old_locus_tag="pgaptmp_003688"
     CDS             10204..11889
                     /gene="yddA"
                     /locus_tag="ACHKYO_RS18440"
                     /old_locus_tag="pgaptmp_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HGU4430459.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     IAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVPP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEILHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            11927..14299
                     /gene="yddB"
                     /locus_tag="ACHKYO_RS18445"
                     /old_locus_tag="pgaptmp_003689"
     CDS             11927..14299
                     /gene="yddB"
                     /locus_tag="ACHKYO_RS18445"
                     /old_locus_tag="pgaptmp_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGU4430460.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDTWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            14356..17139
                     /gene="pqqL"
                     /locus_tag="ACHKYO_RS18450"
                     /old_locus_tag="pgaptmp_003690"
     CDS             14356..17139
                     /gene="pqqL"
                     /locus_tag="ACHKYO_RS18450"
                     /old_locus_tag="pgaptmp_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU4430461.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            17501..>17742
                     /locus_tag="ACHKYO_RS18455"
                     /old_locus_tag="pgaptmp_003691"
     CDS             17501..>17742
                     /locus_tag="ACHKYO_RS18455"
                     /old_locus_tag="pgaptmp_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4430462.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         5216 a         3842 c         3848 g         4836 t
ORIGIN      
        1 gtagcaaggt tttcctatta aaagagaacc aacatttatt acaaacgcat taagtctgga
       61 aacccggcga ctcgccaatg tattagcaac attaatcgat tgtctgatgt aacggttttc
      121 ccgcttatcc tcttaacaca tggagaatgc atcagatgca aactacgcgt accccctact
      181 ctatttcctt tatggcgaca gtgttacttt tattgctctt tgcttgccat agcaccgttg
      241 ccaacgcagc tgtcgcactg ggtgcgacgc gggtaattta tccggcaaac cagaaacagg
      301 ttttattgcc tgtcacgaat aatgatcctg ccagtgtcta tctcattcag tcatggatag
      361 aaaatgcagg tgaccaaaaa gacacccagt ttgtcattac cccaccgctt ttttccatgc
      421 aaggtaaaaa agagaatacg ctgcggataa tcaacgctac taatcatcag ttaccaggcg
      481 accgtgaaag cctgttctgg gtgaatgtta aagctattcc tgctatggaa aaagaccaga
      541 agaatgagaa tacattacag ctggcaatta ttagccgcat aaaaatgttt tatcgcccca
      601 cgaacctggc aatggcacct gaagaagcgc ctgcaatgct tcggtttcgt cgcagcggta
      661 gcaaactcac gcttattaat ccaacgccct atttcattac cgtaacaaat atgaaagcag
      721 gaaatagcaa tctcccaaac accatggtgc cgccaaaagg tgaagtatcg gtggatatca
      781 cgcatgctgc aaccggggat atcagttttc agacgatcaa tgattacggt gcattaactc
      841 cgcgtattaa ggcaaccatg cagtaagcca gtgacctaat taataataaa ctcaatcgga
      901 attaataatg acagcctttc gtgccgcatt taaggcatat cgtatgcatc aggtgttaat
      961 aatgcctcgc tttgcacggc ttaccattgc ccttggctta gcaactgcgg tatttcccgt
     1021 tgacgcagag tattatttta atccacggtt tttaagcaat gatcttgctg aatctgtcga
     1081 tttatccgcc tttactaagg gacgcgaggc accacctggt acatatcggg tcgacattta
     1141 tttgaacgac gaatttatga ctagtcggga tattactttt attgcagatg ataataacgc
     1201 agaactcatc ccatgcctga gtacagatct tcttgtcagt cttggtataa aaaaaatagc
     1261 gttattagac aataaagaac attcggcaga aaaacatgtg ccggacaaca gcgcatgcac
     1321 accacttcag gatcgtttgg ccgacgcatc aactgagttt gatgttggtc agcaacattt
     1381 gtcactctcc gttccgcaaa tttatgttgg cagaatggct cgcggctatg tttctcctga
     1441 tctgtgggaa gaaggaataa atgctgggct tctaaactat agttttaatg gtaattctat
     1501 taataatcgt agtaaccata atgcaggaaa atccaactat gcatatttga atttacagag
     1561 tggcatcaac attggtagtt ggcgactgcg cgataactca acgtggagtt attacagtgg
     1621 gagtagcaat tcatctgaca gcaataaatg gcagcatatc aatacgtcgg ctgaacgtga
     1681 cattattccc ttacgctcac ggttaacggt aggtgatagt tataccgatg gcgatatttt
     1741 tgatagtgtg aactttcgtg gcctcaaaat aaattcaaca gaagcgatgt tgcccgatag
     1801 ccaacatggt ttcgctccgg tgattcatgg tattgcccgt ggaaccgcac aagtgagtgt
     1861 aaaacagaac ggatacgatg tttatcagac tactgttcca cccggccctt ttactattga
     1921 tgatatcaac tctgcggcca atggtggtga tctgcaagta accataaaag aggcagacgg
     1981 cagtattcag acattatatg ttccttattc gtctgttccg gttctccaac gtgctggata
     2041 tacgcgttat gcgcttgcca tgggggaata tcgtagtgga aataacctgc aaagctctcc
     2101 caagttcata caaggtagct tgatgcatgg tctggaagga aactggacac cttatggcgg
     2161 aatgcaaatt gcagaagatt atcaggcctt caaccttggt attgggaaag atttaggact
     2221 ttttggtgcc ttttctttcg atatcacgca ggccaatacg acacttgcag atggcacccg
     2281 tcacagtggg caatcggtta aatccgtcta cagcaaatcc ttctaccaga caggaaccaa
     2341 tatccaggtc gcaggatatc gctattctac gcaaggtttt tataacttat ccgacagtgc
     2401 ctacagtcga atgagtggtt acaccgtcaa gcctcctacc ggagacacca atgagcagac
     2461 acaatttatt gattatttta atctgttcta cagtaagcgt ggtcaggaac aaataagcat
     2521 ctctcagcag cttggaaatt acggtacgac atttttcagt gccagtcgcc aaagttactg
     2581 gaacacgtca cgcagcgacc agcaaatatc atttggatta aatgtgccgt ttggtgatat
     2641 tacgacttcg ctgaattaca gctattccaa taatatatgg caaaacgatc gggatcattt
     2701 actcgctttt acgcttaatg ttcccttcag tcattggatg cgtacagaca gtcagtcggc
     2761 atttcgtaat tcaaacgcca gttacagtat gtcaaacgat ttgaaaggcg gcatgaccaa
     2821 tctatcgggg gtttatggca ctctgctgcc ggataataac ctgaattata gcgttcaggt
     2881 cggtaacacc cacggaggta atacatcgtc tggcaccagt ggttacagtt ctcttaatta
     2941 tcgtggagct tatggtaata ctaatgtcgg ttacagtcgg agtggtgaca gcagccagat
     3001 ttattacgga atgagtggtg ggattattgc tcatgctgat ggcatcacct ttggacagcc
     3061 gctgggcgac acaatggttc tggttaaggc tcctggtgct gataatgtca aaatagagaa
     3121 ccagaccgga attcataccg actggcgtgg ctatgccata ttaccatttg cgacagaata
     3181 tagagaaaac cgtgttgctc ttaacgcgaa ttcccttgca gataatgttg aactggatga
     3241 aaccgtggtc actgtcatcc caactcacgg tgctattgcc agagcaacat ttaatgcaca
     3301 aatcggcggg aaagtattaa tgacgttgaa gtacggtaat aagagcgttc cattcggtgc
     3361 aattgtcaca cacggagaga ataaaaatgg cagcattgtc gcagaaaacg gtcaggttta
     3421 tctgactgga cttccacagt cagggaaatt acaggtttca tggggcaaag ataaaaactc
     3481 aaactgtatt gtcgagtaca agcttcctga agtttctcct ggtaccttgc tgaaccagca
     3541 gacagcaatc tgtcgctaag gaacaataga ttatgaaata caataacatt attttcctcg
     3601 gtttatgtct ggggttaacc acctattctg ctttatccgc agatagcgtt attaaaatta
     3661 gcgggcgcgt cctcgattat ggctgcacag tctcatcgga ttcgcttaat tttaccgtag
     3721 atctccaaaa aaacagtgcc agacaatttc caacgaccgg tagcacaagt ccagccgtcc
     3781 cttttcagat tacgttaagt gaatgcagca aagggacaac gggggttcgg gttgcattta
     3841 acggtattga ggatgcagaa aataatactt tgttgaaact ggatgaagga agcaatacgg
     3901 cctccggttt gggtatagaa atattggacg caaatatgcg tccggtgaaa ctgaatgacc
     3961 ttcatgccga gatgcagtgg atcccactgg taccagaaca gaacaatatt ttgccttact
     4021 ccgctcgtct gaagtcaact cagaagtccg tcaatccggg actggtgagg gcttcggcaa
     4081 cctttaccct tgaatttcaa taatcagagt cagatatgaa aagacttcac aagaggttcc
     4141 tgttagctac gttttgcgcg ttattcacag caactctcca ggccgccgat gtcactatca
     4201 ctgttaatgg tcgggtagtc gctaaaccct gcactattca aaccaaagaa gctaacgtta
     4261 atctcgggga tctttatacg cgcaatctgc aacaacctgg ttctgcatct ggctggcaca
     4321 atattacttt gtcattaacc gattgtccgg ttgaaacaag tgcagtgacg gcaatcgtga
     4381 caggttcaac tgacaatacg ggttattaca aaaatgaagg tactgccgaa aatattcaga
     4441 tagagctgag ggatgaccag gatgctgcgt taaaaaatgg cgatagcaaa acggttattg
     4501 ttgatgagat cactcgtaat gcacagtttc cacttaaggc aagagctatc acggtgaatg
     4561 gaaacgcaag ccagggaacg atcgaggcgc taatcaatgt gatctacacc tggcaataac
     4621 gtcgattcga ttttcttagc cttcttttta aatcaatttt taaccttagg atatatcatg
     4681 ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc ttctattaat ggcaggcaaa
     4741 gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta ttggagcagg tacaacctct
     4801 gtttatgtta atctcgaccc tgtaatacag ccgggccaga atctggttgt agacttgtct
     4861 cagcatataa gttgctggaa tgattacggc ggctggtacg acactgatca tataaacctg
     4921 gtacaaggct cagctttcgc aggctcccta cagtcatata aaggatcgct gtactggaat
     4981 aatgtgacct accccttccc gttgacgaca aataccaacg tattggatat tggtgataag
     5041 acaccgatgc cattaccgtt aaaactctat attaccccag ttggggcggc cggtggagtt
     5101 gtcataaaag cgggggaagt cattgcccgt attcatatgt ataaaattgc aacattagga
     5161 agcggaaatc cgcgtaattt tacgtggaat attatttcaa ataacaatgt tgttatgcca
     5221 acaggcggct gcaccgttga tagtcgtaat gtcaccgtgg atctcccgga ctttcctgga
     5281 agcgcagaaa taccccttgg cgtatattgc tcaagcgaac aaaagctctc attttatctt
     5341 tcgggtgcca ccactgacag tagccgacaa gtgtttgcta acacagcacc tgatgcaaca
     5401 aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa aaatactggc tacgggagaa
     5461 aatgtctccc tggggacagt aaataaaagc aaagtacctc tgggtttaag cgcaacctat
     5521 ggacaaacag gaaacaaggt ttcagccgga accgtccagt cagtcattgg tgtcactttt
     5581 atctatgaat aattgtgatt ttgaccttag tctatgcgct ccattcacga aaattgcgcg
     5641 gataattatt ttgtgaaggc tattagccta cacctgtaag aaaatccgcg cacaattgaa
     5701 gccagagtct tatttttatg ttaatttaaa cactatgaga gatgatgctt aacttatcgt
     5761 atttaatcta ttgtttaacg ataatagatt aaaaatagaa agagagtagt cccctactct
     5821 tatatatcca tgttggcgat aatccctttg tatgtactgt gcatcatcgc tattacaaat
     5881 cctaataatt catttccaca caggataagt agatgaagaa aaaaattgaa tcctaccagg
     5941 gtgctgcagg tggttggggt gctgtcaaat ccgtagcgaa tgcagtacgt aagcagatgg
     6001 atatacgcca ggatgttatt gccatgtttg acatgaataa tctacaatgc actaacatcc
     6061 agacagtgac ggtgcctgtt cctgcgaagt ttttacgtgc ttcaaatgta ccgactggat
     6121 tgcttaatga aatgattgct tatctcaact cggaagaacg caatcatcat aatttttcag
     6181 aacttttgct tttttcttgc ctgtctattt ttgccgcatg caaaggtttc attacactat
     6241 taactaacgg tgtgctatcc gtttccggaa aagtgagaaa tattgtcaac atgaagctgg
     6301 cgcacccatg gaagctgaaa gatatttgtg actgcctgta catcagtgaa agcctgttga
     6361 agaaaaaact aaagcaagag caaacgacat tctcacagat tctattagat gccagaatgc
     6421 agcacgcaaa aaatttgata cgcgtagaag gttcagtcaa taaaattgcc gaacaatgtg
     6481 gttatgccag tacatcttat tttatttatg cgttccgcaa acatttcggc aacagtccga
     6541 agagagtttc taaggagtac cgttgtcaaa gtcacacggg tatgaatacg ggcaacacga
     6601 tgagtgcttt agctatttga ttatttgcta acgagtagtc aaccacgcac gctacgtaag
     6661 aattatatgg ggcagccatt ccctgccccg cgttgttttt aggcgatata tttattgaaa
     6721 taaataagtg acatccatca catatttatg cacttgcata acctgttgca tgattattta
     6781 tgatctcaat tcggcatttt gtcagtaaaa tgcaataatt tattaaatat caataaatta
     6841 gttgtttatc ggcgagaaat tacttaatag aacagaaagt aatgtcaacg ctttatggac
     6901 tgttatttcc ctttttttag ctaaatctgc tatctcttta tgtgactaac ttcacttaca
     6961 tccacttatt tctcttcgta aaattacttc ggaattaagt acaataagaa gaggaacatt
     7021 tatgaagtct gcattaaaga aaagtgtcgt aagtacctcg atatctttga tactggcatc
     7081 tggtatggct gcatttgctg ctcatgcggc agatgatgta aagctgaaag caaccaaaac
     7141 aaacgttgct ttctcagact ttacgccgac tgaatacagt accaaaggaa agccaaatat
     7201 tatcgtacta accatggatg atcttggtta tggacaactt ccttttgata agggatcttt
     7261 tgacccaaaa acaatggaaa atcgtgaagt tgtcgatacc tacaaaatag ggatagataa
     7321 agccattgaa gctgcacaaa aatcaacgcc gacgctcctt tcattaatgg atgaaggcgt
     7381 acggtttact aacggctatg tggcacacgg tgtttccggc ccctcccgtg ccgcaataat
     7441 gaccggtcga gctcccgccc gctttggtgt ctattccaat accgatgctc aggatggtat
     7501 tccgctaaca gaaactttct tgcctgaatt attccagaat catggttatt acactgcagc
     7561 agtaggtaaa tggcacttgt caaaaatcag taatgtgccg gtaccggaag ataaacaaac
     7621 gcgtgactat catgacaact tcaccacatt ttctgcggaa gaatggcaac ctcaaaaccg
     7681 tggctttgat tactttatgg gattccacgc tgcaggaacg gcatattaca actccccttc
     7741 actgttcaaa aatcgtgaac gtgtccccgc aaaaggttat atcagcgatc agttaaccga
     7801 tgaggcaatt ggcgttgttg atcgtgccaa aacacttgac cagcctttta tgctttacct
     7861 ggcttataat gctccgcacc tgccaaatga taatcctgca ccggatcaat atcagaagca
     7921 atttaatacc ggtagtcaaa cagcagataa ctactacgct tccgtttatt ctgttgatca
     7981 gggtgtaaaa cgcattctcg aacaactgaa gaaaaacgga cagtatgaca atacaattat
     8041 tctctttacc tccgataatg gtgcggttat cgatggtcct ctgccgctga acggggcgca
     8101 aaaaggctat aagagccaga cctatcctgg cggtactcac accccaatgt ttatgtggtg
     8161 gaaaggaaaa cttcaacccg gtaattatga caagctgatt tccgcaatgg atttctaccc
     8221 gacagctctt gatgcagccg atatcagcat tccaaaagac cttaagctgg atggcgtttc
     8281 cttgctgccc tggttgcaag ataagaaaca aggcgagcca cataaaaatc tgacctggat
     8341 aacctcttat tctcactggt ttgacgagga aaatattcca ttctgggata attaccacaa
     8401 atttgttcgc catcagtcag acgattaccc gcataacccc aacactgagg acttaagcca
     8461 attctcttat acggtgagaa ataacgatta ttcgcttgtg tatacagtag aaaacaatca
     8521 gttaggtctc tacaaactga cggatctaca gcaaaaagat aaccttgccg ccgccaatcc
     8581 gcaggtcgtt aaagagatgc aaggcgtggt aagagagttt atcgacagca gccagccacc
     8641 gcttagcgaa gtaaatcagg agaagtttaa caatatcaag aaagcactaa gcgaagcgaa
     8701 ataactaaac cttcatgcgg cggaattttc cgccgcctta ttgagcgaga tagcgatgca
     8761 cgttacagcc aagccctcca gttttcaatg taatctcaaa tgtgattact gtttttacct
     8821 tgaaaaagag tcgcagttta ctcatgaaaa atggatggat gacagcactc tgaaagagtt
     8881 catcaaacaa tatatcgcag cgtctggcaa tcaggtctat tttacctggc aaggcggtga
     8941 acccactctg gctggcctgg attttttccg taaagttatt cactatcaac aatgctatgc
     9001 aggccaaaaa cgtattttta atgcattaca aacgaatggc attttattga ataatgaatg
     9061 gtgtgctttt ctcaaagaac atgagtttct ggttggtatc tcgatcgatg gcccccagga
     9121 gctacatgac cgttacagac gcagtaattc aggtaacggt acttttgcaa aagtgatagc
     9181 agccatcgag cgtctgaaat catatcaagt tgagtttaat acgttaaccg tcattaataa
     9241 cgttaatgtc cattaccctc ttgaggttta tcatttttta aaatctatcg gcagtaaaca
     9301 tatgcaattt atcgaattgc tcgaaaccgg gacgccgaat attgatttca gtggtcatag
     9361 tgagaacaca ttccgtatca ttgatttttc tgtgcctccc acggcttatg gcaagtttat
     9421 gtcaaccatt tttatgcaat gggttaaaaa cgatgtgggt gaaattttca tccgtcagtt
     9481 tgaaagcttt gtcagccgtt ttttagggaa tgggcatacc agttgtattt tccaggagtc
     9541 ctgcaaggat aatctggttg ttgaaagtaa tggagatatt tatgaatgcg accattttgt
     9601 ctatccacag tacaaaattg gaaacattaa taaatctgaa ctcaaaacga tgaacagtgt
     9661 acaactgaca gcgcaaaaaa aacggatttc agcgaaatgt ctgaaatgtg catataaacc
     9721 tatctgcaat ggcggttgtc ctaagcatcg tattactaaa gtaaacaatg agactgtttc
     9781 ttatttttgc gaaggttata aaatcctttt ttcaaccatg gtaccttata tgaacgccat
     9841 ggtggagtta gctaagaaca gagtaccgct ttaccacatt atggatgttg caaaacaaat
     9901 ggagaataat taataacgct ctcttttaac tccattctta gcctccttct tatgacctta
     9961 acaaggcacc cgacataaat tcctgtcggg tgtttcgtca tcatcatctt cccttgcgaa
    10021 aatcctggct tacgtggtat gcgtcagggc attagcaata cccttgatta tcaatagcat
    10081 aaccataacc actgaaaatg aaatattcct ttatatttca tattgttaaa tgatacttaa
    10141 ataaatttca tcagtaaatc attgccatag ataatcattg gatggcacaa tcaattttcg
    10201 ctaatgataa ccattcccat tacgttttgt atgttgatag caaagtatct ctgtctgctg
    10261 aagccctttt ggctgcgtaa gaataataaa acttcagtgt tgttaatcat tattatcctc
    10321 gccatgatcc ttggcgtggt taaaattcag gtctggttaa acgactggaa taatgatttt
    10381 ttcaatgcgc taagccagaa agaaaccgat aagctttggc agcttgtcct ttggttccct
    10441 gccctgctgg ggatctttgt attaatatcc gtaaacaaaa catggttaat taaactactg
    10501 actattcgct ggcgcgaatg gttaacagat tactacctta atcggtggtt tgcagataaa
    10561 aactactact tcacgcagat ctacggcgag cataaaaata cagataaccc tgaccaacgt
    10621 atcgctgaag acattctctt actgatcagc aaaacgctca gcctttcatt tggcttcatc
    10681 cagtcactta gcatgctgat cacctttacc gttattcttt ggcaaagcgc gggtacgctc
    10741 tcattcactg ttggtggaac agaatggaat atccaggggt atatggtcta taccgttgtg
    10801 ctcattgtga tcggtggaac tttatttacc cataaggttg gtaaacgtat tcgtccgctt
    10861 aatgtggaaa aacaacgtag cgaagcgaca tttcgaacta atcttgtgca gcataataaa
    10921 caggcagaac tgattgcgct aagcaatgct gagtctctac aacgccagga gttgagcgac
    10981 aattttcaca ccattaaaga gaactggcat cgtttaatga atcgtcagcg gtggcttgat
    11041 tactggcaga atatttattc gcgttcgctt agcgttcttc cgtactttct gttattgccg
    11101 cagtttatta gcggacagat caatctgggc ggacttatga aatcgcgcca ggcatttatg
    11161 ctggtatcga acaatttaag ctggtttatt tataaatatg atgaaattgc tgaactggct
    11221 gcggttatcg atcgcttgta tgagttccat caactcaccg aacagcgccc tacgaataag
    11281 cctaaaaatt gccaacatgc ggtacaagtg gctgacgcga gtattcgtac ccctgataat
    11341 aagatcatat tagagaacct gaactttcat gttccgccag gcaaatggct attactgaaa
    11401 ggttactctg gtgcgggaaa aaccacactg cttaaaacat tatcccactg ctggccgtgg
    11461 tttaaaggtg atatttcttc tcctgccgac agttggtatg tgtcacaaac accgttaatc
    11521 aaaaccggct tactgaaaga gattatttgt aaagcacttc ctctgcccgt agacgataaa
    11581 tcgttgagcg aaatactgca tcaggttggt ctggggaaat tggctgcgcg tattcatgac
    11641 cacgatcgct ggggagatat tctttccagc ggcgaaaaac aacgtatcgc cctggcacga
    11701 ttaattttac gacgtccgaa atggatattt cttgacgaaa ctacctctca tcttgaggaa
    11761 caagaggcta tccgcttact gcgtttagtg cgtgaaaaac tacccacaag cggcgtcatt
    11821 atggttacac atcaacccgg tgtctggaac ctggccgatg atatttgtga cattagcgcg
    11881 gttttataaa aacaataaaa gccctgctgt acggagatat aaataaatga agcgagttct
    11941 tattcctggc gtcattttat gtggcgctga tgtggcgcag gccgtcgatg acaaaaacat
    12001 gtacatgcat gtttttgaag agatgacggt ctatgctcct gtccctgtac ccgtaaacgg
    12061 caacacgcat tacaccagtg aaagcatcga gcgtttaccg accgggaatg gcaatatcag
    12121 cgatctgctg agaaccaacc ctgcggtacg catggattca acgcaaagta cctcgttgaa
    12181 ccagggagat attcgcccgg agaaaatctc tattcacggt gcgtcgccct accagaatgc
    12241 ctatttgatt gatggtatta gtgccactaa taacctgaac ccagcgaatg agtccgatgc
    12301 cagtagtgca accaatatta gcgggatgtc acaggggtat tatcttgatg tcagcttact
    12361 ggacaatgtg acgctttatg acagttttgt gccggttgaa ttcggtcgct tcaatggcgg
    12421 ggtaattgat gcaaagatca aacgcttcaa cgctgatgat agcagcgtga aactgggtta
    12481 tcgcactacg cgttcggact ggttaacatc gcatatcgat gagaataaca agagcgcatt
    12541 taatcaaggt tcttcaggaa gtacttatta ctctccagat tttaaaaaga acttttatac
    12601 cttgtcgttt aatcaggaac tcgctgataa ctttggcgtt accgccggtt tatcgcgccg
    12661 ccagtctgat atcacccgcg cggattatgt ttcgaatgac ggcattgtcg ccggtcgggc
    12721 acagtataaa aacgttatcg atactgcatt gagcaaattt acctggtttg ccagcgaccg
    12781 ctttacccac gatttaacct taaaatatac cggctccagc cgtgattata ataccagcac
    12841 cttcccgcag tctgatcgcg aaatgggtaa taaatcctat ggtctggcat gggatatgga
    12901 tacgcagctc gcatgggcca aacttcgtac caccgttggt tgggatcata ttagtgatta
    12961 tacccgtcac gatcatgaca cctggtacac cgaactttca tgtacatatg gtgatattac
    13021 agggcgttgc acccgtggcg gattaggaca catttcccag gctgtagata attacacctt
    13081 caaaacacgc ctggactggc aaaaattcgc cgtgggtaat gtttcgcatc aaccctactt
    13141 cggcgcggaa tacatctatt ccgatgcatg gactgaacgc cataaccagt ctgaatccta
    13201 tgtgattaat gctgccggaa agaaaactaa ccataccatt taccataaag gtaaaggcag
    13261 cctgggaatt gacaactaca cactgtatat ggcggatcgc attagctggc gtaatgtgtc
    13321 attaatgccc ggcgtgcggt atgactatga caactatctg tcaaaccaca atatctcccc
    13381 gcgctttatg acggaatggg atatttttgc tgatcaaacc tcaatgatta ccgcaggtta
    13441 taaccgttac tatggtggga atattcttga tatgggatta cgtgatatcc gcaatagctg
    13501 gacggaatcg gtatcaggca ataaaaccct gacgcgttat caggatttga aaacgcctta
    13561 taacgatgaa ctggcaatgg gattgcagca gaaaatcggt aagaacgtta ttgcgcgtgc
    13621 aaactatgtt taccgtgaag cgcatgatca aatcagcaaa agcagtcgta ccgacagcgc
    13681 gactaaaacc accattactg aatataacaa cgatggcaaa accaaaacgc attcgttcaa
    13741 cctcagtttt gagctggccg aacccctgca tatccgccag gtagatatta acccacaaat
    13801 tgtctttagc tatatcaaga gcaagggcaa cttgtcgtta aacaatggtt atgaggagag
    13861 caataccggt gataaccagg tggtttataa cggtaatctt gtctcttacg atagcgttcc
    13921 agtggcagat tttaataacc cattaaagat ctccttaaac atggatttca cgcatcaacc
    13981 gagcgggttg gtgtgggcga atacgctggc ctggcaagaa gcgcgtaaag ctcgcattat
    14041 cctgggtaag acaaatgcgc aatacatcag cgaatattca gattacaagc agtatgttga
    14101 cgaaaaactg gatagcagcc tgacctggga cacccgcttg tcctggacgc cacaatttct
    14161 gaaacaacaa aacctgacga tcagtgccga tattctcaat gtattggata gcaaaaccgc
    14221 tgttgataca acgaataccg gtgtggcgac ctacgccagt ggccgtactt tctggcttga
    14281 tgtcagcatg aaattttaat agttcggatt ttacttaacc gggtaaattc gcccggtttt
    14341 cgcatggaga taattatgag aaacctctgt ttcttactga cgttagtggc aactctgttg
    14401 ctccccgggc ggctgattgc cgccgcctta ccgcaggatg aaaagttaat taccgggcaa
    14461 ctgggcaatg gcttgcgata tatgatttat ccgcatgctc atccaaagga tcaggtaaat
    14521 ttatggctgc aaattcatac cggttcattg caggaagaag acaatgagcg cggcgtggct
    14581 cattttgtag aacatatgat gtttaacggc acaaaaacat ggccgggtaa taaagtcatc
    14641 gaaacatttg agtcaatggg cctgcgtttt ggtcgcgatg ttaatgccta taccagctat
    14701 gacgaaacgg tgtatcaggt gagtttgccg actacgcaga aacaaaatct gcaacaagtg
    14761 atggcaatct tcagtgaatg gagtaatgcc gcaacctttg aaaaactcga agtagacgct
    14821 gaacgtggcg taattactga ggaatggcgt gcccatcagg atgcgaaatg gcgcacctct
    14881 caggcgcgcc gccctttcct gctggctaat acccgtaatt tagaccgtga acctatcggc
    14941 ctgatggata ctgtcgccac ggtcacaccg gcacaattgc gccaatttta tcaacgctgg
    15001 tatcaaccaa ataatatgac ctttatcgtg gtcggcgata tcgacagtaa agaagcgctg
    15061 gcgctgataa aggataattt aagtaagctt ccggctaaca aagcagctga aaatcgcgtc
    15121 tggccgacaa aagccgaaaa ccacctgcgc tttaatatca ttaatgataa agaaaaccgg
    15181 gtgaacggca tcgcactcta ttatcgcctg ccaatggtac aagtgaacga tgagcaaagc
    15241 tttatcgaac aagctgaatg gagcatgtta gttcagctgt tcaatcaacg tctgcaggaa
    15301 cgcatacagt cgggcgagtt gaagactatt tctggcggca ctgcgcgcag cgttaaaatt
    15361 gcacccgatt atcagtcgct gtttttccgt gtaaatgcac gagacgataa tatgcaggat
    15421 gctgcgaatg cattaatggc agagttggca accattgatc agcatggttt ttctgctgaa
    15481 gaactcgatg atgtcaaatc tacccgcctc acctggctga aaaatgcggt tgatcagcaa
    15541 gctgaacgtg atttacgtat gctgaccagt cgcctggcat ccagctcatt aaataatacg
    15601 ccgttcttgt cgccggaaga gacatatcaa ctttcgaaac gtctgtggca gcaaattacc
    15661 gtgcaaagtc tggcggaaaa atggcagcag ttaagaaaga accaggacgc attttgggag
    15721 caaatggtaa acaatgagct tgccgccaaa aaagcattgt ctcctgcggc tatcctggcg
    15781 ctggaaaaag agtacgccaa caaaaagctg gcggcttaca tcttcccagg cagaaattta
    15841 tcgttaacag tagacgctga cccacaggcg gaaattagca gcaaagaaac gctggctgag
    15901 aatctgacat cattaacact ttccaatggt gccagggtta ttctggcaaa atccgcgggt
    15961 gaagagcaaa agctacaaat tactgccgta tcgaataaag gcgatttaag tttccctgcg
    16021 cagcaaaaat cacttatcgc gctggcaaat aaagcagtaa gcggaagcgg cgttggtgag
    16081 ctctcctctt ccagcctgaa acgctggagt gcggaaaatt cggtaaccat gagcagtaaa
    16141 gtcagtggca tgaatacgtt gctctccgtt agcgcgcgga ctaataaccc tgaacctggt
    16201 tttcagttga ttaaccagcg aatcacccac agcacgatta acgacaatat ttgggcatcg
    16261 ctacaaaatg ctcaaattca ggcgttgaaa acgctcgacc agcgtccagc ggagaaattc
    16321 gcccagcaga tgtatgagac gcgctatgct gatgaccgca cgaaattact gcaagaaaat
    16381 cagattgtac agtttactgc cgcagatgcg ctggctgccg atcgccagtt gttttcatct
    16441 ccagcggata tcacgtttgt cattgtcggt aatgtctcag aagacaaact cgtggcgtta
    16501 attacgcgtt acttaggatc aatcaaacac tctgattcgc cattagccgc aggtaaacca
    16561 ttaactcgcg cgacggacaa cgcatcggtt actgtaaaag aacaaaatga acctgtggca
    16621 caggtttcac agtggaagcg ttatgattcc cggacacctg ttaatctggc gacgcgtatg
    16681 gcgctcgatg cttttaacgt cgcactggca aaagatctac gtgttaatat tcgtgaacag
    16741 gcatctggag catacagcgt ttcttctcgc ctctcggttg atcctcaggc caaagatatc
    16801 agtcatttgc tggcttttac ttgtcaacca gaacgacatg atgaactgtt aacgttagcg
    16861 aatgaagtga tggttaagcg tctggctaaa gggatcagtg agcaagaact gaatgaatac
    16921 cagcaaaacg ttcagcgcag cctcgatatc caacagcgta gcgttcaaca attagcgaac
    16981 actattgtaa atagtcttat tcaatatgac gatcctgcag catggactga gcaggagcaa
    17041 ttgttgaaac aaatgacggt agagaatgtt aacactgccg ttaaacaata tctttctcat
    17101 ccggtaaata cttataccgg agtattattg ccaaaataat aacagccccg tcaacacatc
    17161 gttggcgggg attttagcaa tattcgctat ttttatgtaa taattttata aatgcgttca
    17221 aaataataat caagtactaa tagtgatatt ttaaggtctg atttttacgt gataattcag
    17281 gagacacaga atgcgcataa aaataacagc ataaaacacc ttaccaccac ccaagaattt
    17341 catattgcat tgtttttcaa tgaaaaaata ttattcgcgt aatatctcac gataaataac
    17401 attaggattt tgttatttaa acacgagtcc tttgcacttg cttactttat cgataaatcc
    17461 tactttttta atgcgatcca atcattttaa ggagtttaaa atggataaga agcaagtaac
    17521 ggatttaagg tcggaactac tcgattcacg ttttggtgcg aagtctattt ccactatcgc
    17581 agaatcaaaa cgttttccgc tgcacgaaat gcgcgacgat gtcgcattcc agattatcaa
    17641 tgacgaatta tatcttgatg gcaacgctcg tcagaacctg gccactttct gccagacctg
    17701 ggacgacgaa aatgtccaca aattgatgga tttatccatt aa
//
LOCUS       DAZGAN020000099        17684 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGAN020000099 DAZGAN020000000
VERSION     DAZGAN020000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.099"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..218)
                     /locus_tag="ACHKYO_RS18460"
                     /old_locus_tag="pgaptmp_003692"
     CDS             complement(<1..218)
                     /locus_tag="ACHKYO_RS18460"
                     /old_locus_tag="pgaptmp_003692"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430463.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGEL"
     gene            complement(243..2876)
                     /gene="mngB"
                     /locus_tag="ACHKYO_RS18465"
                     /old_locus_tag="pgaptmp_003693"
     CDS             complement(243..2876)
                     /gene="mngB"
                     /locus_tag="ACHKYO_RS18465"
                     /old_locus_tag="pgaptmp_003693"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HGU4430464.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(2894..4810)
                     /gene="mngA"
                     /locus_tag="ACHKYO_RS18470"
                     /old_locus_tag="pgaptmp_003694"
     CDS             complement(2894..4810)
                     /gene="mngA"
                     /locus_tag="ACHKYO_RS18470"
                     /old_locus_tag="pgaptmp_003694"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HGU4430465.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            4979..5701
                     /gene="mngR"
                     /locus_tag="ACHKYO_RS18475"
                     /old_locus_tag="pgaptmp_003695"
     CDS             4979..5701
                     /gene="mngR"
                     /locus_tag="ACHKYO_RS18475"
                     /old_locus_tag="pgaptmp_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HGU4430466.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(5805..6674)
                     /gene="sucD"
                     /locus_tag="ACHKYO_RS18480"
                     /old_locus_tag="pgaptmp_003696"
     CDS             complement(5805..6674)
                     /gene="sucD"
                     /locus_tag="ACHKYO_RS18480"
                     /old_locus_tag="pgaptmp_003696"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HGU4430467.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(6674..7840)
                     /gene="sucC"
                     /locus_tag="ACHKYO_RS18485"
                     /old_locus_tag="pgaptmp_003697"
     CDS             complement(6674..7840)
                     /gene="sucC"
                     /locus_tag="ACHKYO_RS18485"
                     /old_locus_tag="pgaptmp_003697"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HGU4430468.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(7934..9151)
                     /gene="odhB"
                     /locus_tag="ACHKYO_RS18490"
                     /old_locus_tag="pgaptmp_003698"
     CDS             complement(7934..9151)
                     /gene="odhB"
                     /locus_tag="ACHKYO_RS18490"
                     /old_locus_tag="pgaptmp_003698"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HGU4430469.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(9166..11967)
                     /gene="sucA"
                     /locus_tag="ACHKYO_RS18495"
                     /old_locus_tag="pgaptmp_003699"
     CDS             complement(9166..11967)
                     /gene="sucA"
                     /locus_tag="ACHKYO_RS18495"
                     /old_locus_tag="pgaptmp_003699"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HGU4430470.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPTFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(12268..12984)
                     /gene="sdhB"
                     /locus_tag="ACHKYO_RS18500"
                     /old_locus_tag="pgaptmp_003700"
     CDS             complement(12268..12984)
                     /gene="sdhB"
                     /locus_tag="ACHKYO_RS18500"
                     /old_locus_tag="pgaptmp_003700"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HGU4430471.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(13000..14766)
                     /gene="sdhA"
                     /locus_tag="ACHKYO_RS18505"
                     /old_locus_tag="pgaptmp_003701"
     CDS             complement(13000..14766)
                     /gene="sdhA"
                     /locus_tag="ACHKYO_RS18505"
                     /old_locus_tag="pgaptmp_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HGU4430472.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(14766..15113)
                     /gene="sdhD"
                     /locus_tag="ACHKYO_RS18510"
                     /old_locus_tag="pgaptmp_003702"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(14766..15113)
                     /gene="sdhD"
                     /locus_tag="ACHKYO_RS18510"
                     /old_locus_tag="pgaptmp_003702"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HGU4430473.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(15107..15511)
                     /gene="sdhC"
                     /locus_tag="ACHKYO_RS18515"
                     /old_locus_tag="pgaptmp_003703"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(15107..15511)
                     /gene="sdhC"
                     /locus_tag="ACHKYO_RS18515"
                     /old_locus_tag="pgaptmp_003703"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU4430474.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            15892..15999
                     /locus_tag="ACHKYO_RS18520"
                     /old_locus_tag="pgaptmp_003704"
     CDS             15892..15999
                     /locus_tag="ACHKYO_RS18520"
                     /old_locus_tag="pgaptmp_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430475.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16205..17488
                     /gene="gltA"
                     /locus_tag="ACHKYO_RS18525"
                     /old_locus_tag="pgaptmp_003705"
     CDS             16205..17488
                     /gene="gltA"
                     /locus_tag="ACHKYO_RS18525"
                     /old_locus_tag="pgaptmp_003705"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HGU4430476.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         4206 a         4794 c         4386 g         4298 t
ORIGIN      
        1 attaactcac ccagggttat tattttgtta aaaataaagc cacatatacg tggcttgaga
       61 gtgaagataa acgcaaaatt acctgatgcg ctacgcttgc cagtgtctac atcatctctg
      121 caatatattg aatttgcctg cttttgtaga ccgaataaag cgttcatgcc gtatccggca
      181 tgaacaaagc gccctttgtc aacaatctgg ccgcgcatat gtggcttcgg tttgattgct
      241 attcaggcaa gccggtaact gaacgtccgt gactggccgg gtaaaaaagg ccccgataga
      301 tttgaacctt gattttcctc ggtggtaatg tgttcatcca tcatcgtttc tgagcaagaa
      361 atcacctcgc gactgaaagc aacagtcgca tcacaggttg ctgattcagc cggattaaac
      421 agccgtaaaa tcacttcttg tcggtcttca gctttcttaa gtgcgcttat caggcatccc
      481 actgggggca ttttcaacaa actataactt tccggcacgt tgaatccggc tttgttgagc
      541 ttcatcgcat cccatgggat tttgttgtaa cactgtactg gagtcagcca tgctcgcgcc
      601 tgctgagcta caccagcggc ggttggcgta ccggtataac tcaataaact taggcgacaa
      661 gaaagcagac cacgtagttg tgagtccggg actggcattt taattcccga aggccgccca
      721 ggccttaaaa acagatcttc tttgcccagt aagcccacgc cacgcagcaa cgtaatggca
      781 aaggttttct tctcttcacc gatgacttca aattcacgta gcccttcgct aaagacagcc
      841 atgccgttac gcccttcctg taaggctgca tagttgagca tattccatac cggaaccggc
      901 gcttctttcc agccttcttg ctgccagttg ttcattgcac tgtcgttcac ggggcgcgtt
      961 agcgaaccaa actgggtatc tgccagaaca ctatcggtgt taaaagatgt agggatcagg
     1021 acgcgaaggc gatgatcgtc agcctggtta tcaaggttga tatccacatc aatacgtctg
     1081 ctgttatgac tcagagtgac aaccatctcc acccctactc tgcccgtgga ttgccggacg
     1141 cttcgttcca acaaattgag cggcaccgcc atgtcatagc ggataacagc cctgctctgc
     1201 caggcctcat gagtaatctc gcattgcggt ttcgctgttg cagaagtcat tacccactct
     1261 tcttttgcgg gtgaatagtc atattcatca ccatcatcag agctttcttc aatttgcaac
     1321 acccggtcat agcgcacacc gctatcttta tctaccagtt gtagcgttcc atcctcattg
     1381 agcgcaattt gccagaaagc attttccagt atcccttcag cgtcactttt tgccgcaatc
     1441 acgttgccag gctgattcgc ttcgatataa agcgtgcgat agcccataga agggacaatc
     1501 tggttgatct gtatatcaaa ctccataaag ggatcgtaat taccgtaatg aactatttgc
     1561 cgatcgatta ggcctggatc gatctcacgg gcatggcgaa taaaatacgg tacaggctga
     1621 ccgcgatcgt ctcgtagatt aaactggctg gcgcgcagcc gcacagtggt gttaataact
     1681 tcttcccgcg gccagggcat caggttaaac agcacgagtt tgtcggcatc actctgtggc
     1741 atgttatcgg caattttgcg catgtagaaa cgcagcagat tatccgccat gtcttcagcc
     1801 agttcgaagc gggcgacaat ttcgcgatga actttgtcac tgcaacaaca accgatactg
     1861 tcgtgggcat gattttttaa gatctctttc cacattttct ccagcaagcc gtggtggtat
     1921 tcaaaaccca acgtccaggc cagcgttgcc agcggttcca gcagattaac aatcttattt
     1981 tcaatacgtg cgtgggcaat tttgatatcc atgcgcgtgg aaccgatggt gcgatgtacg
     2041 cgcatatatt tgccatcgat aaattcacct ttcagggtgg ccagactttc acgctgcgct
     2101 tcgatcttct caaatacctc ttcaaagcgg ctcatcacaa acttacgttg agggtagatc
     2161 tcacgtagct tatccatcac ttcgaagata ttttgctgca atggcatctg gtcatgccca
     2221 ttcggcagca aaatctcttt ggttacagac gctttttcca gcacgtcaaa ataactgtcg
     2281 aggcgtttac gtaatccgtt ttcgtcggca ggtaagtact taccgatggc gtagcccagc
     2341 ggcagcacct gcgccgtcac ttcgctaccg tcactgcttt gccacaaaaa ctcggtttta
     2401 tcagtaccgt ggcgctccga acatccgcgc cagaacatgg tgcgggtaat gccaaatcca
     2461 ttgtagatat gcggcagttg cccggacatg ccaaaggaat ctggtaaata acctattttc
     2521 atcggctcgc caaacgcgag gcagtcacgc attccgtaca tcagattacg gacgatggat
     2581 tccgcagaaa caatcgtggt atcggtctgg gtataccagg ggccgataat caacttgccg
     2641 gcttctacct gtttcttcac gcggtctttg ttttccggtt tcaccgcgaa ataatcttcg
     2701 aggatcgccg tttgcccgtc gagtacgtaa tatttgtatt cgttgtcctg ttccagtcgg
     2761 cacaggatct cttccatatt attgaccagc agaatgcgtg actcttcggt ggtgaaatac
     2821 cactcccgat cccaatgcat atgcggggtg atgtgaacgc gagataccgc tttcatcgtc
     2881 gtttcctttt tgtttatggc attacgccat cagtcagata gttgccatgc ttaaccgcgt
     2941 gacgccgcca gatcaggaga attgcagtcg agattgcagc ccccaccaat gccgcaccaa
     3001 accagccaat tgccgccata acaccgcccg cgccattatc atgaagtaaa aagagcgaga
     3061 aaatgccggc accgggtgtc gaaaggccga tattcatcgc accgacaata gcgcccgtta
     3121 ccatagagcc cagcacaaac gaaccaataa cccgcagcgg atcttcaatc gccatcggga
     3181 tcgccccttc ggtaatacct gccagcccta acagccaggt ggatttcccg gtctcaattt
     3241 caaactcttt aaacaggcgc ggtgcgagca tcgtggaagc ggttacggta aatgccgaaa
     3301 ccattttgac ggaggcgaaa atggcatacg ggccgtaaac gccgttcgcc attgcgccca
     3361 ggcagaatgc ataagcggct ttgttcactg gccctccaag gtcaaaggaa cacataaaac
     3421 cgagaatggc acccagcaac agcgcgttac ttcctgacag accgttcagc caggcggtaa
     3481 gcgagttatt gatccaggcg acaggttctc ccaccacaaa cagcatcaga ctgcccgctc
     3541 ccaacgtacc gagcaccggg tagagataaa aagtcaggaa tccattgaat ttactgctaa
     3601 gacgcaagtg atttttcacc cagcgcatca agtaaccggc tatcaatccg ccaacgaccg
     3661 cgccgagaaa cccggagccg atcatgttgg cggcaagtcc agccgcaaag cctggcgcta
     3721 acgccggttt atctgccaga gaataggcgg tataggccgc gagcaccggt accatcaaaa
     3781 ttccgagcag cccgccgccc agcttgcggt acatccacag ccaggagttt tcttcattaa
     3841 acagatcttg tagcccgaag atttgcgaca gtaataccgc gaccgccagc accgtgcccc
     3901 ccgcgacaat caacggtacg gcaaaagaga ttccgctcaa cagtgcctgt ttcagctccg
     3961 ttttgacact tttcaccggt tgcgtatctt gctgtacggt acgcgtctca tcgctacgct
     4021 tgagggtaag cgcttgttgg atcaacgctt ctgcatggcg aatcggctcg gcaacaggca
     4081 ctgaaagcgc gggaatgcca ttaaaacgct cactctcctt gatggcgact tcagccgcaa
     4141 aaatacaggc ggttgcacta ttgagttgat ccgccgttaa acgcccttca atgccgttag
     4201 cgccttgttt ttcaacgtaa acatttacgc cgagtttgcg tccggctttt tccagatatt
     4261 ccgcagccat ataggtgtga gcaatacccg ccggacaggc cgttacgcag acgatagttg
     4321 gcgcgttgga aaaagaggca gaaggttgcg tgcctccctt gtcatccagc gccgagagca
     4381 actcatcagg cgtcgtcgcc gactgtatac gcgcccgaat ctcatcatcc gccaggcgcg
     4441 tggtcagcgc tgtcagcagt tgcatatgcg tagtacccgc ttcattgggg ggaatcgcca
     4501 gcagcaccac taaatcaacc gcttccgggc catcaacgcc ttcccactga agcggctcgc
     4561 tgagtgtggc gaccgcaaac gccgcttctt ttaccgcagc agttttgcca tgcggcacag
     4621 ccaacccttc acctaaggcg gtcgggccaa ggctttcacg gcgatacact tcttccagaa
     4681 attgctcagt actggaaatt ttccccagag cagcaagacg ttgagtcaac gcgtggatgg
     4741 cctcttcacg gctggtaaag cgcgcattca gacacaacgc atcgcggtgg gtcagagtcg
     4801 tcagattcat catgatcctc acttgatcgt ttttatagtc gcgccagtgt gcccgataaa
     4861 acaataccat tatgtgatca atttcacttg attgaaatta tttgtattgt atttgtatta
     4921 tttacaggat gaatttatcg ggcataatgg ctaaattcgt attaatgaga tactaaaaat
     4981 gggacacaag cccttatacc ggcagattgc cgatcgcatt cgtgaacaaa ttgcgcgtgg
     5041 cgagcttaaa cctggcgatg cgttacctac cgaatcggca ttacaaacag agtttggcgt
     5101 cagccgggtt acggtgcgtc aggctttgcg ccagttagtc gagcaacaga tcctcgaaag
     5161 cattcagggc agcgggactt acgtcaaaga agagcgggtc aattacgata tttttcagtt
     5221 aaccagtttt gatgaaaaac tgtcggaccg tcacgtcgat acacacagtg aagtcctgat
     5281 atttgaagtg attccggctg acgattttct tcagcaacag ctacaaatca ccgcgcagga
     5341 tcgtgtgtgg catgtgaagc gcgtgcgcta tcgcaagcaa aagccaatgg cgctggaaga
     5401 aacctggatg ccgcttgcgt tgttcccgga tctcacctgg caggtcatgg aaaattcaaa
     5461 atatcacttt attgaagaag tgaagaaaat ggttatcgat cgtagcgaac aggaaatcat
     5521 tcctctgatg ccaaccgaag agatgagccg tctgcttaat atcagccaga caaagccgat
     5581 tcttgaaaaa gtatcgcgtg gatatttagt tgatggtcgg gtgtttgaat atagccgcaa
     5641 cgcttttaat accgatgact ataaatttac cctgatagcg caacgaaaat catcgcgata
     5701 agcacaaaaa aggccatcat acgatggcca accatgtcga aaccgacagc ccttcgccat
     5761 tcctgtagag ggaagcggcg agggctattt cttattacag atatttattt cagaacagtt
     5821 ttcagtgctt caccgatatc cgccaggctg cgaacggttt tcacgcctgc ggcttccaga
     5881 gcagcgaatt tctcatccgc agtccctttc ccaccggcaa tgatggcacc cgcgtggccc
     5941 atacgtttgc ctttcggcgc agtcacacca gcgatgtaac ccacaactgg cttggtaaca
     6001 tgctctttga tgtacgctgc cgcttcttct tcagcgctac cgccgatctc accgatcatt
     6061 acgatcgctt cggtctgcgg atctttttcg aacatttcga gaatgtcgat aaagttagag
     6121 ccagggatcg ggtcaccgcc gataccaaca caggtcgact gaccgaaacc gtaatccgtg
     6181 gtctgtttaa ccgcttcata ggtcagtgta ccggaacggg aaacgatacc cactttaccc
     6241 ggtttgtgaa tgtgacccgg ctggataccg attttgcatt cacccggagt gataacgccc
     6301 gggcagttcg ggccgatcat acgaacgccg gcttcgtcca gtttcacttt cacagtcagc
     6361 atatccagcg tcgggatgcc ttcagtgatg gtgataatca gtttgatgcc tgcatcaata
     6421 gcttccagaa tggagtcttt gcagaacggt gctggtacgt agataacaga agcggtagcg
     6481 ccagtagcag caacagcttc acgcacggtg ttgaacaccg gcaggccgag gtgggtagta
     6541 ccgcctttac ctggggttac gccgccaacc attttagtgc cgtatgcaat ggcctgttct
     6601 gagtggaaag tcccctggct accagtaaag ccctggcaga taaccttggt gtttttatca
     6661 attaaaatgg acattatttc ccctccactg cggcaacaac ctgctgagct gcatccgtca
     6721 gaccttttgc tgcaataata ttcaggccgc tgtcagccag tttcttcgcg ccgagttcgg
     6781 cgttgttacc ttccagacgt accacgaccg gtacgttaac acccacttct gctaccgcgc
     6841 cgatgatacc gtcagcgatc aggtcgcaac gaacgatacc gccgaagatg ttaaccagaa
     6901 cggctttcac tttgtcgtca gagaggatga ttttgaacgc ttcggttaca cgttctttgg
     6961 ttgcgccgcc gccaacgtca aggaagttag ccggttcgcc gccgtgcagt ttaacgatgt
     7021 ccatcgtacc catcgccaga cctgcgccgt taaccataca accgatgtta ccgtccagcg
     7081 caacgtagtt cagttcccac tgtgcagcct gtgcttcacg cggatcttcc tgcgactggt
     7141 cacgcatttc gcgcagatca ggctggcgga acagtgcgtt gccgtcagcg cccagtttgc
     7201 cgtcgaggca aatcagatcg ccctgtttgg tgatgaccag cgggttgatt tcgatcaacg
     7261 ccaggtcgcg ctccaggaaa atggtcgcca ggcccatgaa gattttggtg aactgctgaa
     7321 ccagtttacc ttccagaccc agtttgaacg ccagctcgcg tccctgatac ggcatcgggc
     7381 ctgtcagcgg atcaagcgca actttatgga tcaggtgcgg agtttcttcc gccacttttt
     7441 cgatttccac gccgccttcg gtggaggcca taaagaccac gcgacgggaa ctacggtcta
     7501 caacggcacc gagatacagc tctttagcga tatcggtcgc tgcttcaacc agaatctggt
     7561 taaccggttg gccattggca tctgtttgat acgttaccag acgcttgccg agccagtttt
     7621 ctgcaaaagc acggatgtct tctttgctgt ttacaacttt cacaccgccc gctttaccgc
     7681 ggccaccagc gtgaacctga catttcacta cccacgggcc ggcaccgatt tttgaagcgg
     7741 cttcttctgc ttcgcgcgga gtagtacagg cataacccac cggtgctggt aagccatagc
     7801 gggcaaaaag ttgttttgcc tgatattcat gtaagttcat gtgttctgtc catccttcag
     7861 taatcgttat cttttaaacc gtagaccgac accctatcca cgtcggtcta cagtgcaggt
     7921 gaagcttaaa ctactacacg tccagcagca gacgcgtcgg atcttccagc aactctttga
     7981 tcgttaccag gaagcccacg gattcgcgac catcgatcag acggtgatcg taggacagcg
     8041 ccaggtacat catcggcagg atctcaacct gaccattcac cgccatcgga cgatctttga
     8101 tagcgtgcat acccagaatt gcgctctgcg gcgggttgat gatcggcgta gacatcaggg
     8161 aaccgaacac accaccgttg gtgatggtga agttaccacc ggtcagatct tcaacggtca
     8221 gcttgccgtc acggcctttg actgccagct ctttgatttt cttctcgatg tctgccatgc
     8281 cgagggtatc gacatcacgc agtaccggtg tcaccaggcc gcgcggcgta gaaaccgcca
     8341 tgctgacgtc gaaatagttg tggtaaacca cgtcatcgcc gtcgatagac gcgttcactt
     8401 ccgggtaacg tttcagggct tcaaccaccg ctttcacgta gaaggacata aagcccagac
     8461 ggatgccgtg gcgtttttca aacgcttcac cgtactgctt acgcagatcc ataatcggct
     8521 tcatgttgac ttcgttgaac gtggtcagca tggcggtgga gtttttcgct tccagcagac
     8581 gctctgccac acgcttacgc aggcgagtca tcgggacacg tttttcacta cgtgcagcca
     8641 gagccggttg cgccgccgga tcagccgctg ccggagcaga ctctttcgcc ggggctttcg
     8701 ccagatgttt ttccacatct tcacgagtca gacgaccacc cacaccggtg cctttaatgg
     8761 cgctggcgtc gagattgtgt tcagccagca ggcgacggat cgccgggctt aacgcatcgt
     8821 tgttttgctc ttccagagac gcctgctggc gttgcgccgg agtggacgct ttctcttcag
     8881 atttggcgct ggtttcttta ccggcgctgt tgccttcacg caggcgacca aggatctgac
     8941 gagacgttac cgttgtacct tcatcttcca gaaccgcatc cagaatgccg tctgctgatg
     9001 ccggtacttc cagtaccact ttgtcagttt cgatttctac cagcacttca tcacgtacga
     9061 ctgcgtcgcc gggtttttta tgccaggttg cgacggtggc atcggctacg gattcaggca
     9121 ggtcagggac cagaatatct acgctactca ttgtgtatcc tttatttatt cgacgttcag
     9181 cgcgtcatta accagatctt gttgctgttt ctggtgaacg gacatatacc ctaccgccgg
     9241 agaggcggag gccgggcggc ctgcataacg cagagaagcc ccaaacggaa tcacttcacg
     9301 gaaatgatgc tggctgcagt accatgcgcc ctggttgagc ggctcttcct ggcaccagac
     9361 aaaatccttg acgtgagcaa actgctgcaa cacttcctgc atcgctttat gcgggaacgg
     9421 gtagagttgc tcgatacgca caatggcgac atcgtgttga ttgttcttac gacgctgttc
     9481 cagcaggtcg taataaacct taccagaaca cattactacg cgcttcacgc ccttcggatc
     9541 aagctcgtcg atttcaccga tggctggcag gaaggtgccg ttcgccagtt cttcgaggct
     9601 ggaaaccgcc agcggatgac gcagcaggga tttcggcgac atcacgacca gcggacgacg
     9661 catcccgcgc agcgcctgac gacgcagcat gtggtaaacc tgtgccgggg tagacggtac
     9721 gcaaacctgc atgttttgct cagcacaaag ttgcagataa cgttccagac gcgcggagga
     9781 gtgctccggc ccctgccctt cgtaaccgtg cggcagcaac atcaccagac cacacatccg
     9841 gccccatttc tgctcgccag aggagatgaa ctggtcgata accacctgcg caccgttggc
     9901 gaagtcaccg aactgcgctt cccagatagt cagagtgcgt ggttctgcgg tggcataacc
     9961 gtattcaaac gccagcactg cttcttcaga cagaacggag tcccagacac ggaacgcgcc
    10021 ctggccgtta tgaatatgtt gcagcggcgt gtaagtggaa ccgttagact ggttgtggat
    10081 caccgcgtgg cggtggaaga aggtaccgcg accggagtct tcacccgaca ggcgaaccgg
    10141 aatgccttcg tcaaccagcg tggcgtaagc gaggttttcc gcaccgcccc agtcgaacag
    10201 tttctcaccg gcagccatcg cctggcgatc gccataaatc ttggcaacgc gagactgcat
    10261 ttcaactgct tccggcaccg tgctgatgcg tttcgccagc tcctgcaggc gcttcatctc
    10321 aactttgttc gggtactctt cgtcccattc gtggttgagg tacggcgacc aggtgaaaga
    10381 gtgcatgttc atcggacgcc actctgccac tacgcaatcg ccagcatcca gcgcatcgcg
    10441 gtacaggtta accatctcgg tggcatcttc cagcgtcgcg actttttcct gctccagctt
    10501 gtcagcgtag attttgcgcg gtgtcggatg ttttttgatt ttctgataca tcagcggctg
    10561 ggttgcgctc ggctcgtcgg cttcgttgtg gccgtgacgg cggtagcaca ccaggtcgat
    10621 gaagacatca cgtttaaagg tgttacggaa atcgagcgcc agacgggtca caaaggcaac
    10681 ggcttccgga tcatccgcgt taacgtggaa aatcggtgcc tgaaccatct taccgatatc
    10741 agtacagtac ggtgtagaac gagcatccag cggattagag gtggtgaaac caacctggtt
    10801 gttgataacg atacgtaccg taccgccaac ttcataacca cgcgctttcg acatgttcag
    10861 agtttcctga accacgccct gcccggtcac tgcggcgtca ccgtggatgg tgattggcag
    10921 cactttgttg ctgctcggct catcaagtct gtccagacgg gcacgaacag aaccgataac
    10981 taccgggctt acaatctcaa ggtgagacgg gttaaacgcc agcgccaggt gcaccaggcc
    11041 gccatcggtc tggaagtcag acgagaagcc catgtggtat ttcacgtcac ccgtgccgag
    11101 gtgttcttta tgtttaccgg caaactcgtc gaacaagtct tgcggttttt tacccagcac
    11161 gttcaccagc acgttcagac gaccacggtg cgccatccca agaaccactt cgcgggtgcc
    11221 gctgttgcca gcgtggcgga tcatctcttt aagcatcggg attaacgcgt caccgccttc
    11281 cagcgagaag cgttttgcgc cagggaattt tgcgccgagg taacgttcaa ggccttcagc
    11341 ggcggtcagt tcgcttaaga agcgtttttt ctcttcgcta ttgaaagtcg cgcgaccaga
    11401 ctcgatacgc tgttggatcc agcgtttttc ttcggtgctg gtaatgtgca tatactcggc
    11461 accaatcggg ccacagtagg tttgcttgag ggcttccagc agctcgccga gtttcatggt
    11521 ttctttgccg ctggcaaatg aaccgacgtt gaaggtttcc tggaagtctg cttcggtcag
    11581 atcgtggaaa gtcggatcca gatcggccac tttatcttgc tgccacagtc ccagcggatc
    11641 gagattcgca tgctggtgac cacggaagcg gtatgcgtta atgagctgca ggactttaac
    11701 ctgcttcaca ttggtgtcag ggtcggagat cgttgaagag taacgtgaag cgtctttcgc
    11761 caggcggcgg aaatattcac gcgtttgaga gtggaattga tccggtttga ctcccgtacc
    11821 aggtaactgc tggaacgtcg aacgccagtt agcgtcaacc gagtcaggat cggttaagaa
    11881 gtcttcatag agctgttcta tccagctctg gtttgcgcca gagaggtaag aagagtccaa
    11941 ccaggctttc aaagcgctgt tctgcatcgt gatcccttaa gcatcttttt tatgcttact
    12001 tcgccgtgga tactaccacg cacagtgatg tgcgtgccgg ggttcacttg cgagctcttg
    12061 tctatgtagt ggctcgcgaa ggaaccttta aaaactgtct tagtgtcagg ggcagttttt
    12121 aaagatttcc tgcatttctt tgttacgcct gatgcgctgc gcttatcagg cctacgggta
    12181 atgacttgta ggccggataa ggcgttcacg ccgcatccgg cactggttgc ctgatgcgac
    12241 gcttgcgcgt cttatcaggc ctacggttta cgcattacgt tgcaacaaca tcgacttaat
    12301 atggccgatg gcgcgcgtcg ggttcagccc cttcggacat acactgacgc agttcatgat
    12361 gctgtgacag cggaatacgc tgaatgcatc gctcaaaccg tcgaggcggc tgtcagtctc
    12421 ggtatcacgg ctatcgatca ggaaacgata tgccgctaac aagcctgccg ggccgataaa
    12481 cttatcggga ttccaccaga aagacggaca agaggttgaa caacatgcgc agagaataca
    12541 ttcatacagc ccgtcgagtt tttcgcgctg ctctggcatc tgtaaatgct cgcgagctgg
    12601 cggattttgt ccattattca acaggtaagg cttaattttc tcatattgcg catagaattg
    12661 tcccatgtct accaccaaat cgcggatcac cggtaaacct ggcagcgggc gaatcacaat
    12721 cttcttgccc ggctggttga gtgccgaaat cggggtaata caggccagac cattcttgcc
    12781 gttcatgttc agaccgtcgg aaccgcacac accttcacgg caggagcggc ggaacgacag
    12841 gctgggatct ttctctttca gctggataag cgcatccagc agcatcatgt cgcgaccttc
    12901 ttccgcttcc agggtgtaat cctgcatacg cggagcatca tcaacatccg ggttatagcg
    12961 ataaattgaa aactcgagtc tcattttcct gtctccgcat tagtaagtac gaatcttcgg
    13021 cgggaatgcc gggcgcagtt tcggttccat gttgacgctt cggcgcgtca tggattccga
    13081 ctcaggcaga tacagggagt ggcacagcca gttttcatca tcacgatccg ggaagtcgaa
    13141 gcggctatgt gcgccacggc tttcggtacg gaagttggca gaaacagccg ttgcatacgc
    13201 cgtttccatc aggttatcca gttccaggca ctcaacgcgc tgggtgttga actcgctgga
    13261 agtgtcatcc agacgggcat ttttcagacg ctcgcggatc actttcaact gctcaagccc
    13321 tttcgccatc gcatcacctt cacggaagac cgagaagtta tgctgcatac attcttgcag
    13381 cgctttacga atcgccaccg gatcttcacc gttacgatta ttgttccagc ggttcagacg
    13441 atccagagat gcttcaacat cagactcgct ggcatcgcgc aatgcgccct gttcggcgat
    13501 agactcttgc agatgcagac ccgctgcacg accaaagacc accaggtcca gcagcgagtt
    13561 gccgcccaga cggttagcgc cgtgtaccga tacacaagcg atttcaccaa cggcaaacag
    13621 tcccggaaca accacatctt cgcctttctc gttcaccgtc agcgcctgac cggtcacttt
    13681 ggtcggaata ccgcccatca tgtagtgaca ggttgggata accggaatcg gctctttcac
    13741 cggatcaacg tgagcgaagg tacgggagag ttccaggata cccggcaggc gggattcgag
    13801 aacttcttta cccaggtggt cgagtttcag tttcgcgtgt ggcccccacg gaccatcaca
    13861 gccgcgacct tcacggattt cgatcatgat ggaacgcgcc accacatcac ggcccgccag
    13921 gtctttggcg ttcggcgcat aacgctccat aaagcgttcg ccatgtttgt tcagcagata
    13981 accgccttca ccacggcaac cttcggtgac cagtacgccc gcaccggcaa taccggtcgg
    14041 gtggaactgc cacatttcca tatcctgcac cggtacgcct gcgcggatag ccatgccgac
    14101 gccgtcgcca gtgttaatgt gggcgttagt ggtggactga taaatacgcc ctgccccgcc
    14161 agtcgccagc acggtagcgc gggctttgaa ataaaccact tcaccagttt cgatgcacag
    14221 tgcagtacaa cccaccaccg cgccatcctg gtttttcacc agatccagcg cataccactc
    14281 ggagaaaatg gtggtgtggt ttttcaggtt ctgctgataa agcgtgtgca acagtgcgtg
    14341 accggtacgg tcagccgccg ccgcagtgcg tgccgcctgc tcgccgccga agtttttcga
    14401 ctgaccgcca aacggacgtt gatagatacg accatcatca agacgcgaga acggcaggcc
    14461 catgtgttcg agttccagaa tcgcttccgg cccggtttta cacatatatt caatcgcgtc
    14521 ctggtcaccg atatagtccg acccttttac ggtgtcgtac atatgccatt cccagttatc
    14581 ttcatgggta ttacccagcg caacggtaat gccgccttgc gcagaaacgg tatgggaacg
    14641 ggtcgggaag actttagaga gcagcgcaca ggtctggccg ctctgggaaa tttgcagcgc
    14701 cgcgcgcata cctgcgccac cggcaccaat cacaactgca tcaaattctc tgactggcaa
    14761 tttcatcaca caccccacac cacaacgaat ccataaatca cgtaaaccac cagtgcaacg
    14821 acaatcacca gttgcagcat caggcgcaat gccagcggtt taacgtagtc ggtcaacacc
    14881 tgccacatgc cgatccaggc atgaatcaag atagaaaaca gcgccagcag ggtgaacact
    14941 ttggtgaacg cagaggcgaa gaaaccgatc cagacttcat atgtcagttc gccactggtg
    15001 gcgaaaaaac cgaccatata aatgatgtag agcgtcagga cgatagcggt agcgcgaacg
    15061 aggatgaaat catgtacgcc attgcgtcct aatgcggagg cgttgcttac catacgagga
    15121 ctcctgcgag aagtgaaagc acgacagtaa taacaaagga gattttggcg gagcgtttac
    15181 ccgcttcgaa tgtttcttcc agatagccaa aatccatcat catgtggcga atacctacga
    15241 cgacgtgata cgccagagcg gtaaggatgc cccacatgat aaatttgacg aagaagctgc
    15301 ccataatcgc ggaagcttgc tcgaaacctt caggggaaga gaggctggta cccagaagcc
    15361 acagcaggat gcccactgca acaaaggtga tcacaccgga aacgcgatgg agaatggacg
    15421 ctatcgccgt gatggggaac cggatggtct gtaggtccag attaacaggt ctttgttttt
    15481 tcacatttct tatcatgaat aacgcccaca tgctgttctt attattccct ggggactacg
    15541 ggcacagagg ttaactttct gttacctgga gacgtcggga tttccttcct ccggtctgct
    15601 tgcgggtcag acagcgtcct ttctataact gcgcgtcatg caaaacactg cttccagatg
    15661 cgaaaacgac acgttacaac gctgggtggc tcgggattgc agggtgttcc ggagacctgg
    15721 cggcagtata ggctgttcac aaaatcatta caattaacct acatatagtt tgtcgggttt
    15781 tatcctgaac agtgatccag gtcacgataa caacatttat ttaattttta atcatctaat
    15841 ttgacaatca ttcaacaaag ttgttacaaa cattaccagg aaaagcatat aatgcgtaaa
    15901 agttatgaag tcggtatttc acctaagatt aacttatgta acagtgtgga agtattgacc
    15961 aattcattcg ggacagttat tagtggtaga caagtttaat aattcggatt gctaagtact
    16021 tgattcgcca tttattcgtc atcaatggat cctttacctg caagcgccca gagctctgta
    16081 cccaggtttt cccctctttc acagagcggc gagccaaata acaaacgggt aaagccaggt
    16141 tgatgtgcga aggcaaattt aagttccggc agtcttacgt aataaggcgc taaggagacc
    16201 ttaaatggct gatacaaaag caaaactcac cctcaacggg gacacagctg ttgaactgga
    16261 tgtgctgaaa ggcacgctgg gtcaagatgt tattgatatc cgtactctcg gttcaaaagg
    16321 tgtgttcacc tttgacccag gcttcacttc aaccgcatcc tgcgaatcta aaattacttt
    16381 tattgatggt gatgaaggta ttttgctgca ccgcggtttc ccgatcgatc agctggcgac
    16441 cgattctaac tacctggaag tttgttacat cctgctgaat ggtgaaaaac cgactcagga
    16501 acagtatgac gaatttaaaa ctacggtgac ccgtcatacc atgatccacg agcagattac
    16561 ccgtctgttc cacgctttcc gtcgcgactc acatccaatg gcagtcatgt gtggtattac
    16621 cggcgcgctg gcggcgttct atcacgactc gctggatgtt aacaatcctc gtcatcgtga
    16681 aattgccgcg ttccgcctgc tgtcgaaaat gccgaccatg gccgcgatgt gttacaagta
    16741 ttccattggt cagccatttg tttatccgcg caacgatctc tcctatgccg gtaacttcct
    16801 gaatatgatg ttctccacgc cgtgcgaacc gtatgaagtt aatccgattc tggaacgtgc
    16861 tatggaccgt attctgatcc tgcacgctga ccatgaacag aacgcctcta cctccaccgt
    16921 gcgtaccgct ggctcttcgg gtgcgaaccc gtttgcctgt atcgcagcag gtattgcttc
    16981 actgtgggga cctgcgcacg gtggtgctaa cgaagcggcg ctgaaaatgc tggaagaaat
    17041 tagctccgtt aaacacattc cggaatttgt tcgtcgtgcg aaagataaaa atgattcttt
    17101 ccgcctgatg ggcttcggtc accgcgtgta caaaaattac gacccgcgcg ccaccgtaat
    17161 gcgtgaaacc tgccatgaag ttctgaaaga gctgggcacc aaagatgacc tgctggaagt
    17221 ggctatggag ctggaaaaca tcgcgctgaa cgacccgtac tttatcgaga agaaactgta
    17281 cccgaacgtc gatttctact ctggtatcat cctgaaagcg atgggtattc cgtcttccat
    17341 gttcaccgtc attttcgcaa tggcacgtac cgttggctgg atcgcccact ggagcgaaat
    17401 gcacagtgac ggtatgaaga ttgcccgtcc gcgtcagctg tatacaggat atgaaaaacg
    17461 cgactttaaa agcgatatca agcgttaatg gttgattgct aagttgtaaa tattttaacc
    17521 cgccgttcaa atggcgggtt aatttttata tgcctaaaca caaaaaattg taaaaataaa
    17581 atccactatc ggacattcat atattctaaa aaaaaatata tcggtataaa ttataaacaa
    17641 atcgatgatt tttaatcatc tatcttcctc tcagttacat gaat
//
LOCUS       DAZGAN020000100        17622 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGAN020000100 DAZGAN020000000
VERSION     DAZGAN020000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.100"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            274..1326
                     /gene="yhiM"
                     /locus_tag="ACHKYO_RS18530"
                     /old_locus_tag="pgaptmp_003706"
     CDS             274..1326
                     /gene="yhiM"
                     /locus_tag="ACHKYO_RS18530"
                     /old_locus_tag="pgaptmp_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HGU4430477.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1642..2844)
                     /gene="yhiN"
                     /locus_tag="ACHKYO_RS18535"
                     /old_locus_tag="pgaptmp_003707"
     CDS             complement(1642..2844)
                     /gene="yhiN"
                     /locus_tag="ACHKYO_RS18535"
                     /old_locus_tag="pgaptmp_003707"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGU4430478.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DFIAAKSS"
     gene            3076..4575
                     /gene="pitA"
                     /locus_tag="ACHKYO_RS18540"
                     /old_locus_tag="pgaptmp_003708"
     CDS             3076..4575
                     /gene="pitA"
                     /locus_tag="ACHKYO_RS18540"
                     /old_locus_tag="pgaptmp_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HGU4430479.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4646..4981)
                     /gene="uspB"
                     /locus_tag="ACHKYO_RS18545"
                     /old_locus_tag="pgaptmp_003709"
     CDS             complement(4646..4981)
                     /gene="uspB"
                     /locus_tag="ACHKYO_RS18545"
                     /old_locus_tag="pgaptmp_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HGU4430480.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5372..5806
                     /gene="uspA"
                     /locus_tag="ACHKYO_RS18550"
                     /old_locus_tag="pgaptmp_003710"
     CDS             5372..5806
                     /gene="uspA"
                     /locus_tag="ACHKYO_RS18550"
                     /old_locus_tag="pgaptmp_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HGU4430481.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(5905..5958)
                     /locus_tag="ACHKYO_RS18555"
                     /old_locus_tag="pgaptmp_003711"
     CDS             complement(5905..5958)
                     /locus_tag="ACHKYO_RS18555"
                     /old_locus_tag="pgaptmp_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430482.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            6124..7593
                     /gene="dtpB"
                     /locus_tag="ACHKYO_RS18560"
                     /old_locus_tag="pgaptmp_003712"
     CDS             6124..7593
                     /gene="dtpB"
                     /locus_tag="ACHKYO_RS18560"
                     /old_locus_tag="pgaptmp_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HGU4430483.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7642..8394)
                     /gene="rsmJ"
                     /locus_tag="ACHKYO_RS18565"
                     /old_locus_tag="pgaptmp_003713"
     CDS             complement(7642..8394)
                     /gene="rsmJ"
                     /locus_tag="ACHKYO_RS18565"
                     /old_locus_tag="pgaptmp_003713"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HGU4430484.1"
                     /translation="MKICLIDETGTGDGALSVLAVRWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8402..10444)
                     /gene="prlC"
                     /locus_tag="ACHKYO_RS18570"
                     /old_locus_tag="pgaptmp_003714"
     CDS             complement(8402..10444)
                     /gene="prlC"
                     /locus_tag="ACHKYO_RS18570"
                     /old_locus_tag="pgaptmp_003714"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HGU4430485.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10647..11489
                     /gene="rlmJ"
                     /locus_tag="ACHKYO_RS18575"
                     /old_locus_tag="pgaptmp_003715"
     CDS             10647..11489
                     /gene="rlmJ"
                     /locus_tag="ACHKYO_RS18575"
                     /old_locus_tag="pgaptmp_003715"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HGU4430486.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATDIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11561..12913
                     /gene="gorA"
                     /locus_tag="ACHKYO_RS18580"
                     /old_locus_tag="pgaptmp_003716"
     CDS             11561..12913
                     /gene="gorA"
                     /locus_tag="ACHKYO_RS18580"
                     /old_locus_tag="pgaptmp_003716"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGU4430487.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FTLPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(12967..13050)
                     /gene="dinQ"
                     /locus_tag="ACHKYO_RS18585"
                     /old_locus_tag="pgaptmp_003717"
     CDS             complement(12967..13050)
                     /gene="dinQ"
                     /locus_tag="ACHKYO_RS18585"
                     /old_locus_tag="pgaptmp_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HGU4430488.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13559..13912
                     /gene="arsR"
                     /locus_tag="ACHKYO_RS18590"
                     /old_locus_tag="pgaptmp_003718"
     CDS             13559..13912
                     /gene="arsR"
                     /locus_tag="ACHKYO_RS18590"
                     /old_locus_tag="pgaptmp_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HGU4430489.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            13966..15255
                     /gene="arsB"
                     /locus_tag="ACHKYO_RS18595"
                     /old_locus_tag="pgaptmp_003719"
     CDS             13966..15255
                     /gene="arsB"
                     /locus_tag="ACHKYO_RS18595"
                     /old_locus_tag="pgaptmp_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HGU4430490.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15268..15693
                     /gene="arsC"
                     /locus_tag="ACHKYO_RS18600"
                     /old_locus_tag="pgaptmp_003720"
     CDS             15268..15693
                     /gene="arsC"
                     /locus_tag="ACHKYO_RS18600"
                     /old_locus_tag="pgaptmp_003720"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HGU4430491.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(15816..16112)
                     /locus_tag="ACHKYO_RS18605"
                     /old_locus_tag="pgaptmp_003721"
     CDS             complement(15816..16112)
                     /locus_tag="ACHKYO_RS18605"
                     /old_locus_tag="pgaptmp_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGU4430492.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            16220..17221
                     /locus_tag="ACHKYO_RS18610"
                     /old_locus_tag="pgaptmp_003722"
     CDS             16220..17221
                     /locus_tag="ACHKYO_RS18610"
                     /old_locus_tag="pgaptmp_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGU4430493.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPIVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         4091 a         4504 c         4412 g         4615 t
ORIGIN      
        1 gcaccgagac tatccggtct tttactaaat aatggaaaac aatccatccc tggaagataa
       61 ttgtaaaaaa aacaacagaa taagaattaa tcttaggata aatatttatt tatcatggcc
      121 ttttaaccgg taaaaaatgg cactcggcac agtaaacccc gctatagtta tgctatgcat
      181 ttgtctttaa tgatatctcg tcgttatatt aacaggctga aattatcatt aatgcacatt
      241 tcattattag cagggatacc tgaggagttt aaagtgaaca tatatatcgg gtggcttttc
      301 aaattaatcc ctttgattat gggattaatt tgcatcgcgt taggtggctt tgtgctggaa
      361 agttcagggc aaagcgagta tttcgtcgcg ggtcatgtgc tgatttctct ggcggccata
      421 tgcctggcat tattcactac cgcatttatt atcatttcgc agctcacgcg cggcgttaat
      481 acgttttaca atacattgtt ccccattatt ggctatgcgg ggtcaattat caccatgata
      541 tggggttggg cactgttagc aggcaatgat gtgatggcag acgagtttgt cgccggccat
      601 gttattttcg gcgttggtat gattgccgcc tgtgtatcga cggtggcagc gtcatccggt
      661 cactttctgc tcattcccaa aaatgcagcg gggagcaaga gcgatggaac gccggtacag
      721 gcttattctt cattaatcgg taattgcctc attgccgttc ccgttttact caccctgctc
      781 ggtttcattt ggtctattac gctgttacgt agtgctgaca taactccgca ttatgtcgcg
      841 ggtcacgtat tgcttgggtt aaccgcaatc tgtgcctgtc taattggcct tgttgccaca
      901 attgtccatc aaacacgtaa tacgttttca actaaagaac actggctgtg gtgttattgg
      961 gttatttttc tcggctcaat cacggtactg caggggatat acgtcttagt cagttccgat
     1021 gcaagcgccc gactggctcc cggcattatt cttatttgcc tcggaatgat ctgttacagc
     1081 atattctcaa aagtctggct actggcactg gtatggagac gtacctgttc gttagccaac
     1141 agaataccga tgattcccgt cttcacctgc ctgttttgcc ttttcctggc atcgtttctt
     1201 gcggaaatgg cgcagaccga catgggatat tttattcctt cgcgggtttt ggtcggttta
     1261 ggagcggtat gctttacgtt gttctcaatc gtttcaatat tagaagcagg ttctgctaaa
     1321 aaataattgc aacgtaccgg ataaaaccag cgttgaccat ttgcgtaacg ctggtttttc
     1381 ttaggcatca tgaaataacg cagcattaat gcatagtggt taaggataaa aaaacaacaa
     1441 agttactgtt tttttcaacc tgttcatatt ttataaagat ctggccactg ggtgagtttt
     1501 caatcacctt tccttccacc ttatattaag cctggagggt ttcagttcac gggctcatta
     1561 gaaaataatc acaagatagc cactatcaaa attagctcat ttttaatgcg gctctaataa
     1621 ttttcatctt taggtaacag gtcaggacga cttcgctgca ataaaatcct gcgcacaagc
     1681 ccacgcactc gaccacgccc actggaagtt atagcccccc agccagccgg taacgtccat
     1741 cacttcgccg atgaagtaca gcccaggcac tttgcgcgct tccatcgtcc gtgaagagag
     1801 ttcgttggtg tccacgccgc cgagcgtcac ttcggcagtg cgatagcctt cagtgccgtt
     1861 gggttgtacg cgccagtcgg tcaatgtgct aatcagtgcc tgttggtcac gcacgttgag
     1921 ctgttttagc gaaacatccg ggatttgccc gagttgctgt aagcgttcaa ccaaccgctt
     1981 cggtagatga accgccagtg tgtttttcag gctctgattc ggatgtgcgt tacgctgctc
     2041 attcaggaag gtttcgagat ccacatccgg tagcagattg atactgacaa attcacccgg
     2101 ttgccagtag ctggaaatct gcaacaccgc cggtccagac aagccgcgat gggtgaagag
     2161 taagttctca cggaaaacga tgccgttttc agcggtaatc acggaaggca ccgccacgcc
     2221 cgccagcacc tgtaactctt cgagcaacgg tttatgcaga gtgaatggca ccagacccgc
     2281 gcgggtcggc agcacgttga ggccaaattg ttcggcaatc ttataaccaa acggcgacgc
     2341 gcccagcccc ggcattgaca gcccaccggt cgcgatgacc agcttttcgc aaccgacagt
     2401 catgccgttc agttcaagcg tgaagcctgt ttcatccttc gccacactca gcacttcgct
     2461 acgcaatctg aaggttacat tgcccttctc gcactcatcc accagcatgt cgacaatctg
     2521 ctgcgcggag tcatcgcaga agagttgtcc taacgttttc tcgtgccagg cgatgccgtg
     2581 tttattgacc agatcaatga aatcccactg ggtaaaacgc gcgagtgcag acttacaaaa
     2641 atgcggattc tggctcagat aagcgcctgg ttcgacataa aggttggtaa agttgcagcg
     2701 cccaccgcca gacataagga ttttgcgccc tggtttttta ccattatcga tcagcagaac
     2761 ccggcgtcct gcctgacctg ccagcgcaga acagaacata cccgccgcac cagcgcctat
     2821 aataatggca tcaaaccttt ccacgttgcg ctcctcttag aaaaaacggg cgtgaattgt
     2881 aaagattcct cagtggtcgc accagcatca atattactaa aaggaagtat ttgcctgaat
     2941 tatataagat aattattttt tgagtgaaat ccatacagga ggcaaatcaa aaaaagtcta
     3001 tatttcactt tgcccgcgcc gcgaaagtca ctgataatgc gccgcgttca tgtcctcaaa
     3061 atggcgtaac gtcctatgct acatttgttt gctggcctgg atttgcatac cgggctgtta
     3121 ttattgcttg cactggcttt tgtgctgttc tacgaagcca tcaatggttt ccatgacaca
     3181 gccaacgccg tggcaaccgt tatctatacc cgcgcgatgc gttctcagct cgccgtggtt
     3241 atggcggcgg tgttcaactt tttgggtgtt ttgctgggtg gtctgagtgt tgcctatgcc
     3301 attgtgcata tgctgccgac ggatctgctg cttaatatgg gatcgtctca tggccttgcc
     3361 atggtgttct ctatgttgct ggcggcgatt atctggaacc tgggtacctg gtactttggt
     3421 ttacctgcat ccagctctca tacgctgatt ggcgcgatca tcgggattgg tttaaccaat
     3481 gcgttgatga ccgggacgtc agtggtggat gcactcaata tcccgaaagt attaagtatt
     3541 ttcggttctc tgatcgtttc ccctattgtc ggcctggtgt ttgctggcgg tctgattttc
     3601 ttgctgcgtc gctactggag cggcaccaag aaacgcgccc gtatccacct gaccccagcg
     3661 gagcgtgaaa agaaagacgg caagaaaaag ccgccgttct ggacgcgtat cgcgctgatc
     3721 ctttccgcta tcggcgtggc gttttcgcac ggcgcgaacg atggtcagaa aggcattggt
     3781 ctggttatgt tggtattgat tggcgtcgcg ccagcaggct tcgtggtgaa catgaatgcc
     3841 actggctacg aaatcacccg tacccgtgat gccatcaaca acgtcgaagc ttactttgag
     3901 cagcatcctg cgctgctcaa acaggctacc ggtgctgatc agttagtacc ggctccggaa
     3961 gctggcgcaa cgcaacctgc ggagttccac tgccatccgt cgaataccat taacgcgctc
     4021 aaccgcctga aaggtatgtt gaccaccgat gtggaaagct acgacaagct gtcgcttgat
     4081 caacgtagcc agatgcgccg cattatgctg tgcgtttctg acactatcga caaagtggtg
     4141 aagatgcctg gcgtgagtgc tgacgatcag cgcctgttga agaaactgaa gtctgacatg
     4201 cttagcacca tcgagtatgc accggtgtgg atcatcatgg cggtcgcgct ggcgttaggt
     4261 atcggtacga tgattggctg gcgccgtgtg gcaacgacta tcggtgagaa aatcggtaag
     4321 aaaggcatga cctacgctca ggggatgtct gcccagatga cggcggcagt gtctatcggc
     4381 ctggcgagtt ataccgggat gccggtttcc actactcacg tactctcctc ttctgtcgcg
     4441 gggacgatgg tggtagatgg tggcggctta cagcgtaaaa ccgtgaccag cattctgatg
     4501 gcctgggtgt ttacccttcc ggctgcggta ctgctttccg gcgggctgta ctggctctcc
     4561 ttgcagttcc tgtaatcgta cacaccaaaa cgagcgggtc agtttactgg cccgcttttt
     4621 ttatgcgtta ttattgctga agtgcttaat gccaaatcat caatgcaatc aggctgacca
     4681 ccaccagacc acacaatgcg ctggtcagaa taaactgccg acgcacccgc tcacagcggc
     4741 gaataaattc atcatcgtga tgatcgcgat aacgttgggc atagatatac caaacgagac
     4801 gcacctgttt gttgggttgg ccatgtgagg taaaaaagcc ccctccatca acatattgat
     4861 agagcaatgg atcgcagtta cgcagtacca ctaacaacgc gcgtagtgat gagaaatagc
     4921 gcgccatgtt aacaatgcaa acgacacata aagcccaaaa taatgcgacg gtgcttatca
     4981 tacctcctcc ccggcgacct gcccgcggag ttccaccccg gggctaccgc tcccgatacg
     5041 ctgccaatca gttaacacca ggtcctggag aaaccgcttt tgtggtgacc aacatccgag
     5101 cggctctata gatagtgtag gagatcaggt tgtttttttt ccagaaggtt aaccactatc
     5161 aatatattca tgtcgaaaat ttgtttatct aacgggtaag caaggcggat tgacggatca
     5221 tccgggtcgc tataaggtaa ggatggtctt aacactgaat ctttacggct gggttagccc
     5281 cgcgcacgta gttcgcagga cgcgggtgac gtaacggcac aagaaacgct agctggccag
     5341 tcatcgacaa ctttatggaa ggagtaacac tatggcttat aaacacattc tcatcgcagt
     5401 cgatctctcc ccggaaagca aagttctggt agagaaagca gtctctatgg ctcgccccta
     5461 caatgcgaaa gtttctctga tccacgtaga tgtaaactac tctgacctat acaccgggct
     5521 tattgatgtg aatctgggtg atatgcagaa acgcatctct gaagagacac atcatgcgct
     5581 gaccgagctt tccactaatg caggctaccc aatcactgaa accctgagcg gcagcggcga
     5641 cctgggccag gttctggtcg atgcaatcaa gaaatacgat atggatctgg tggtttgtgg
     5701 tcaccaccag gacttctgga gcaaactgat gtcttccgca cgtcagctga tcaacaccgt
     5761 tcacgttgat atgctgattg ttccgctgcg cgacgaagaa gaataatcat ccctccacga
     5821 cgtgttcctg aacgcccgca tatgcgggcg ttttgctttt tggcgcgcct tgttacctga
     5881 tgcagcgtaa accccttatc tggcctacga tctgcgtacg caatcaaaat ctcctgccaa
     5941 cacaacattt aacaccatca tattttccat cattagtgtg atcagctggt tattttctgt
     6001 tgtaatagtg tattaatcta ttcaccgcat caatattaag aatctctgac agatgtaaac
     6061 tttttcgcgc gttatccctt acgcgttcat acttttcagg atggtattgg aaggttaata
     6121 aatatgaata caacaacacc catggggatg ctgcagcaac ctcgcccatt tttcatgatc
     6181 ttttttgtcg agttatggga gcgattcggc tactacggcg tgcagggcgt actggcggtt
     6241 ttcttcgtta aacagcttgg attctcgcaa gagcaggctt ttgtcacttt tggtgctttt
     6301 gctgcgctgg tctatggcct catttccatt ggcggctatg tcggcgacca cctgctgggg
     6361 accaaacgca ccattgttct cggagcactt gtgctggcga ttggctactt catgaccggc
     6421 atgtcgctac ttaagcctga cctgattttc atcgccctgg ggactatcgc tgtcggtaac
     6481 ggcctgttta aagctaaccc agccagcttg ctttcgaagt gctatccgcc gaaagatccg
     6541 cggcttgatg gcgcattcac cctgttctat atgtcgatca atatcggctc gttgatagcg
     6601 ttatcgctgg cccctgtgat cgctgataga ttcggttatt cagtcaccta caacctgtgc
     6661 ggggcggggt taattatcgc attactggtt tacatcgcct gtcgtggaat ggtgaaagac
     6721 attggttctg aacccgactt caagccgatg agcttcagca aactgttgta cgtattactt
     6781 ggcagcgtgg tgatgatctt cgtatgtgca tggctgatgc acaacgtaga agtcgccaat
     6841 ctggtgctga ttgttctctc catcgtcgtc accatcatct tctttcgtca ggcattcaag
     6901 ctggataaaa ccgggcgcaa taaaatgttt gtcgcctttg tcctgatgct cgaagcggtg
     6961 gtgttttaca ttctctacgc ccagatgcca acatcgctga acttctttgc catcaacaac
     7021 gtgcatcatg aaattctcgg tttttccatc aacccggtca gcttccaggc gcttaacccg
     7081 ttctgggtgg tactcgccag cccaatactg gcaggcattt acacgcatct gggtaacaaa
     7141 ggcaaagacc tctcgatgcc gatgaaattt actctcggca tgtttatgtg ctctctgggc
     7201 tttttgacgg ctgcagctgc gggaatgtgg tttgcggatg cccaggggct gacatcgcca
     7261 tggtttatcg tgctggtgta cttattccag agcttaggtg aactgtttat tagcgccctt
     7321 ggcctggcga tgattgctgc cctggtgccg cagcatttga tgggctttat tctcgggatg
     7381 tggttcctga cgcaggctgc cgcgttcttg ctgggcggct atgtggcaac atttaccgcg
     7441 gtgccggaca acattaccga tccgcttgag acgttgcccg tctataccaa cgtgtttggt
     7501 aagattggtc tggtcacact gggcgttgca gtagtgatgc tgttgatggt gccgtggctg
     7561 aaacgcatga ttgcgacgcc ggaaagccat taattattct tgcagaaagc agggtagcgt
     7621 tatcgctacc ctggtttact tttacactgg cgtacctgca taaatatcaa acctatgccc
     7681 tttagtgact accgcgtttg gcgtggcgac attcgccagc ggtggcgcgt agtccgggcg
     7741 ctttaccacc acgcgtttgg tcgccagcaa gcgtgcaggc tccagtaatc catcggcatc
     7801 aagatccggt cccaccagcg actgaaagac acgcatctct tttttcacta gcgcgctttt
     7861 ctgcttatgc gggaacatcg ggtcgagata aaccacctgc gggcgcgggg taatatcagt
     7921 cagcgccgtc aggctggagg cgtgaattaa ctgcaaccgc tcctgcaacc agccgccgat
     7981 ttccgcatcc gcataaccac gcgccaggcc gtcgtcgagc aatgcggcaa cgactggatt
     8041 acgctccagc atccgcacgc ggcagccgac tgaagccagc acaaaggcat cgcgccccag
     8101 ccctgcggtg gcatccacca catccggcaa ataatcgcct ttaatgccca ccgctttcgc
     8161 caccgcctca ccgcgaccac cgccgaattt gcgtcggtgc gccatcgctc cgccaacaaa
     8221 atcaacaaag atgccgccaa gttttggctc atcacgcttg cgcaattcca gatgttccgg
     8281 cgttaacacc agcgccatca ggttgtcttc atcgtgctcc agcccccagc ggaccgccag
     8341 aacagataag gcaccgtctc cggtgcctgt ttcatcaatt aagcagattt tcactgaatg
     8401 atcagccctt aatgccgtaa tgctccagca tcgcatccag ctgcggttca cgaccacgga
     8461 agcgtttgaa cagatccatc ggctcttctg aaccgccacg gctcagaatg ttgtcgagga
     8521 acgactgccc ggtttcacgg ttgaaaatgc cctcttcctc aaagcgcgag aaagcatctg
     8581 ccgccagtac gtcagcccac aggtagctgt agtaacctgc ggcataacca ccggcgaaaa
     8641 tatggctgaa agcgtgcggg aaacggcccc aggatggaga cggtactacg gcaaccagtt
     8701 tcttgatttc tgccagagtt tcgaggattt tcgctccctg atccgggcgg aactcggcat
     8761 ggaggcggaa atcgaacagg ccgaactcca gctggcgcag aataaacagc gccgcctggt
     8821 agttcttcgc cgccagcatt ttatccagca actctttcgg cagcggttcg ccggtttcgt
     8881 agtgaccgga gataaacgcc agcgcctccg gctcccagca ccagttttcc ataaactgac
     8941 tcggcagttc gaccgcatcc cacggcacac cgctgatacc ggaaacacca gcggtttcga
     9001 tgcgggtcag catatggtgc aggccgtgac cgaactcgtg gaacagggtg atcacttcgt
     9061 cgtgagtgaa cagcgccggt ttaccattta ccgggcggtt gaagttacaa gtcaaatacg
     9121 cgaccggttt ttgcagcgaa ccatcagctt tacgcatctg gcctacgcag tcatccatcc
     9181 acgccccgcc gcgcttgttt tcacgggcat acagatcgag gtagaagctg ccgcgcagtt
     9241 cgttattttc gtcatacagt tcgaagaaac gtacatccgg atgccagaca tcaacatctt
     9301 tacgctcttt agcggtgatg ccgtaaatac gtttcaccac ttcaaacagg ccgttaaccg
     9361 ctttgttttc tgggaagtac ggacgcagct gctcgtcgct gatgctgtag aggtgctgtt
     9421 tttgtttttc gctgtagtac gcgatatccc acggctgcaa ctcatcgacg ccaaattccg
     9481 ctttggcgaa ggcgcgcagt tgtgccagct ctttttcgcc ttgcggacgc gcgcgttttg
     9541 ccagatcggt taagaaatcc agcacctgct gcgggttttc tgccatttta gtggcaaggg
     9601 atttaaaggc gtagttttca aagcccagca gttgcgccag ttcgtgacgc agagcgagga
     9661 tctcttccat caccttgctg ttatcccatt taccggcgtt cgggccttga tcggaggcgc
     9721 gggtgctgta agcgcgatac atctcttcac gcaaggcctg gttgtcgcag taggtcatta
     9781 ccggcaggta gcttgggata tccagcgtca gcaggtagcc ttccagctct ttcgcttcgg
     9841 cctgggcttt tgccgcagcc agcgcgcttt caggcatccc cgccagctcc gcttcgtcgg
     9901 taacgagttt ggtccagccc atcgtcgcat cgaggacgtt gttgctgtac tggttgccca
     9961 gctcggaaag acgagtggca atctcaccgt agcgttgttg tttctctatt ggcagaccga
    10021 tgccagagag ttcgaagtcg cgcagtgcgt tatcaaccgc ttttttctga gccgtgttca
    10081 gcgtggcgta atgatcgcca tcgcgcaggt cgcgatacgc tttatacagc ccttcatgtt
    10141 gccctaccca ggtgctgtat tccgacagca gcggcagggt ttgttcgtac gcttcacgca
    10201 gttccgggct atttttcacc gagttcaggt ggctgaccgg ggagaagata cgccccagca
    10261 catcgtccac ttccgccaac ggctggcaga gattttccca ggtgtacggt gctccttgcg
    10321 ctactacgcg ctccacattt tcgcggcagt cgttcagcgc cttagtcacg gctggaacga
    10381 catgttccgg gagaatttta gaaaacggag gcaattcaaa gggagtcagt aacggattcg
    10441 tcattcgcgc agtcctggtt aaagaggtta aggaagcgct caacaggcgc tttacataat
    10501 gtgtgtagca tggggttaag tgtagtgaat ttcaatgaga aacgttacgc tttcgcggcg
    10561 gcggcctctt ttcggtatac tgtcctgata cgcttttgtg cgccccgaat acgggccgat
    10621 ttttacttac cggaacacct ttacccatgc tcagttatcg ccacagcttt cacgctggca
    10681 accacgccga cgtccttaaa cataccgttc agagcctgat catcgagtcg ctgaaagaga
    10741 aagataaacc gtttctctat ctcgacaccc acgcaggggc cgggcgttat cagttaggca
    10801 gcgaacatgc cgagcgtacc ggcgaatatc tcgaaggcat cgcccgtatc tggcagcagg
    10861 acgatttgcc cgcagaactg gaggcgtaca tcaatgtggt aaaacacttc aaccgtagcg
    10921 gtcagttgcg ttactacccc ggttcgccgt tgattgctcg ccagctactg cgtgaacagg
    10981 acagcctgca actgaccgaa ctgcacccga gcgattaccc gttgttgcgt tctgaatttc
    11041 agaaagatag ccgtgcgcgt gtcgaaaaag ccgacggttt ccagcagctt aaggccaaac
    11101 tgccgccggt ttcccgccgt ggtttaatcc ttatcgaccc gccgtatgaa atgaaaactg
    11161 actatcaagc ggtggtcagc gggatagcag aaggttacaa acgtttcgcc actggtactt
    11221 acgcactgtg gtatccggtg gtgctgcgtc agcaaattaa gcgcatgatc cacgatctgg
    11281 aagcgaccga tattcgcaaa attctgcaaa ttgaactggc ggtactgcca gacagcgatc
    11341 gccgtggcat gaccgcttcc ggcatgattg tgattaaccc gccgtggaaa ctggaacagc
    11401 agatgaataa cgtgctgccg tggctgcaca gcaaactggt tccggcaggc accgggcacg
    11461 ccaccgtaag ctggatcgtg ccggagtaat tgcagccatt gctggcacct attacgtctc
    11521 gcgctacaat cgcggtaatc aacgataagg acactttgtc atgactaaac actatgatta
    11581 catcgccatc ggcggcggca gcggcggtat cgcctccatc aaccgcgcgg ctatgtacgg
    11641 ccagaaatgt gcgctgattg aagccaaaga gctgggcggc acctgcgtaa atgttggctg
    11701 tgtgccgaaa aaagtgatgt ggcacgcggc gcaaatccgt gaagcgatcc atatgtacgg
    11761 cccggattat ggttttgata ccactatcaa taaattcaac tgggaaacgt tgatcgccag
    11821 ccgtaccgcc tatatcgacc gtattcatac ttcctatgaa aacgtgctcg gtaaaaataa
    11881 cgttgatgta atcaaaggct ttgcccgctt cgttgatgcc aaaacgctgg aggtaaacgg
    11941 cgaaaccatc acggccgatc atattctgat cgccacaggc ggtcgtccga gccacccgga
    12001 tattccgggc gtggaatacg gtattgattc tgatggcttc ttcacccttc ctgctttgcc
    12061 agagcgcgtg gcggttgttg gcgcgggtta catcgccgtt gagctggcgg gcgtgattaa
    12121 cggcctcggc gcgaaaacgc atctgtttgt gcgtaaacat gcgccgctgc gcagcttcga
    12181 cccgatgatt tccgaaacgc tggtcgaagt gatgaacgcc gaaggcccgc agctgcacac
    12241 caacgccatc ccgaaagcgg tagtgaaaaa tgccgatggt agcctgacgc tggagctgga
    12301 agatggtcgc agtgaaacgg tggattgcct gatttgggcg attggtcgcg agcctgccaa
    12361 tgacaacatc aacctggaag ccgctggcgt taaaactaac gaaaaaggct atatcgtcgt
    12421 cgataaatat caaaacacca atattgaagg tatttacgcg gtgggcgata acacgggtgc
    12481 agtggagctg acaccggtgg cagttgcagc gggtcgccgt ctctctgaac gcctgtttaa
    12541 taacaagccg gatgagcatc tggattacag caacattccg accgtggtct tcagccatcc
    12601 gccgattggt actgttggtt taacggaacc gcaggcgcgc gagcagtatg gcgacgatca
    12661 ggtgaaagtg tataaatcct ctttcaccgc gatgtatacc gccgtcacca ctcaccgcca
    12721 gccgtgccgc atgaagctgg tgtgcgttgg atcggaagag aagattgtcg gtattcacgg
    12781 cattggcttt ggtatggacg aaatgttgca gggcttcgcg gtggcgctga agatgggtgc
    12841 aaccaaaaaa gacttcgaca ataccgtcgc cattcaccca acggcggcag aagagttcgt
    12901 gacaatgcgt taaatgttaa agggctaaga gtagtgtgct cttagccctt aattacgttt
    12961 ccgctatcag ttcagaagct gaagcagaaa gcggatcagt tccagcagcg caattaacgc
    13021 cccaagaacg atgattgctt tatcaatcac ccgttttctc catgcgatgg agtgagaatg
    13081 catccgctta ctcatccact gcctgtcacg gcacatgccc tattgttaga taatggcatt
    13141 ctcactccgg ccagagcatc agttaacggc accacccgta cttctgacca ggactttgaa
    13201 agcgtttatg cgcattacca gagtgaaaat gcctcagagc taactggata atcatacagt
    13261 acattcaggt tataaaacca gcatgtcctt gcaatagttt cagtatggta ttagcattga
    13321 tgcgttagat aatggcattc tcactccagc cagtgccacc aactcagggc tggaaagtaa
    13381 aaaaccgacg caaagtcggt ttttttacgt cctgattcag acctcctttc aaatgaatag
    13441 ccaactcaaa attcacacct attaccttcc tctgcactta cacattcgtt aagtcatata
    13501 tgtttttgac ttatccgctt cgaagagaga cactacctgc aacaatcagg agcgcaatat
    13561 gtcatttctg ttacccatcc aattgttcaa aattcttgct gatgaaaccc gtctgggcat
    13621 cgttttactg ctcagcgaac tgggagagtt atgcgtctgc gatctctgca ctgctctcga
    13681 ccagtcgcag cccaagatct cccgccacct ggcattgctg cgtgaaagcg ggctattgct
    13741 ggaccgcaag caaggtaagt gggttcatta ccgcttatca ccgcatattc cagcatgggc
    13801 ggcgaaaatt attgatgagg cctggcgatg tgaacaggaa aaggttcagg cgattgtccg
    13861 caacctggct cgacaaaact gttccgggga cagtaagaac atttgcagtt aaaaatttag
    13921 ctaaacacat atgaattttc agatgtgttt tatccgggag gcattatgtt actggcaggc
    13981 gctatctttg tcctgaccat cgtattggtt atctggcagc cgaaaggttt aggcatcggc
    14041 tggagtgcaa cgctcggcgc agtactggcg ttagttacgg gcgtggtcca tccgggtgat
    14101 attccggtgg tgtggaatat cgtctggaac gcgacggctg cgtttatcgc cgtcattatc
    14161 atcagcctgc tgctggatga gtccggcttt tttgaatggg cggcgctgca cgtctcacgc
    14221 tggggtaatg gtcgtggtcg cttgctgttt acctggattg tcctgctcgg tgctgccgtt
    14281 gccgccctgt ttgccaatga tggcgcggcg cttattttga caccgattgt catcgccatg
    14341 ctgctggctt tagggttcag taaaggcact acgctggcgt tcgtgatggc ggccggattc
    14401 attgccgata ccgccagcct gccgcttatt gtctccaacc tggtgaatat cgtttccgct
    14461 gatttctttg gcctcggctt tcgcgaatac gcctcggtga tggtgccggt ggatatcgcc
    14521 gcgattgttg ccacgctggt gatgttacat ctctattttc gcaaagatat tccgcagaac
    14581 tacgatatgg cgctgctgaa atctcccgca gaagcgatca aagatcctgc tacgttcaaa
    14641 actggctggg ttgttttact gcttctgctg gtgggatttt tcgtcctgga accgctcggc
    14701 attccggtga gcgccattgc agctgtgggc gcgctgatat tatttgtcgt cgctaaacgc
    14761 ggtcatgcga ttaatacggg taaagtcctg cgcggtgccc cctggcagat tgtcatcttc
    14821 tcgctcggca tgtatctggt ggtttatggc ctgcgcaatg ccggattaac ggaatatctt
    14881 tctggcgtac tcaacgtgct ggcggataac ggcctgtggg ccgcgacgct cggcaccgga
    14941 ttcctcaccg ccttcctctc ttctattatg aacaatatgc cgacggtact ggttggcgcg
    15001 ttgtccattg atggcagcac ggcatctggc gttatcaaag aagcgatggt ttatgccaat
    15061 gtgattggct gcgatttggg accgaaaatt accccaattg gtagcctggc tacgctactc
    15121 tggctgcacg tactttcgca gaagaatatg actatcagct ggggatatta cttccgtaca
    15181 gggattatca tgaccctgcc ggtgcttttt gtgacgctgg ctgcgctggc gctacgtctc
    15241 tctttcactt tgtaatgaga tattgatatg agcaacatta ccatttatca caacccggcc
    15301 tgcggtacgt cgcgtaatac gctggagatg atccgcaaca gcggtacaga accgaccatt
    15361 atctattatc tggaaacacc gccaacgcgc gatgagctgg tcaaactcat tgccgatatg
    15421 gggatttccg tacgcgcgct gttgcgtaaa aacgtcgagc cgtatgagga actgggcctt
    15481 gcagaagata aatttactga cgatcggtta atcgacttta tgcttcagca cccgattctg
    15541 attaatcgcc cgattgtggt gacgccgctg ggaactcgcc tgtgccgccc ttcagaagtg
    15601 gtgctggaaa ttctgccaga tgcgcaaaaa ggcgcattct ccaaggaaga tggcgagaaa
    15661 gtggttgatg aagcgggtaa gcggttgaaa taaagcggcg atatccactg cccatagttg
    15721 gtaaagtgcg ctttgtttat gtcggatgcg acgctaacgc gtcttatccg acctacaaaa
    15781 ccgtgcatgg catgaaatct gtagcgcgta gcgcatcagg caattttgct atttacgcag
    15841 cactcctcgc gtaagaattc aattatcgct tccagcattg cgtattgcga aacacacatc
    15901 agcgtccgac tctcacggtt ttgtttaacc agccctaccg ataccagtgc tgaaatatgg
    15961 tgactgagtg tagagccggg tatcccaagc tgcttttgca actcaccgac cggcaaacct
    16021 tgctcccccg ctttgaccag gtgtttgaat atgaacaaac gggtcgggtg acctagctct
    16081 ttcagcgctt tagcaacttc ttccaactgc atatgacacc ccctttgtct ggttgatatt
    16141 tcgataatac cagaaatatc ttgatctgaa tttcatttcg attattctgg aaatatagat
    16201 ttatccaatg aggttacgta tgagtagttg gcttgcgatg ctgcaagatg ccgcagagat
    16261 gtttgtgttt ctcgccgtcg agctttcgtt gctgtttata gtgattagtg ccggtgtcag
    16321 cctgataaga caaaaggtgc cagaccataa aatccagcag atgatggggg ccagaaaagg
    16381 gagaggctat ctcctggctg ctctgttggg agccgttacc ccgttctgta gttgctcgac
    16441 aatacccatg ttacgtggat tgttgtcagc gaaagccggg tttggtccga ctctcacttt
    16501 tttatttgtt tcaccattac ttaatcccat tatcgtcgga ctaatgtgga tgacctttgg
    16561 ttggaaagtc accttgttgt acgcgattat cgccgccggc gtctccgttc ttgccagtat
    16621 tatcctggat tctctggggt ttgaacgtca tatcattgcc agtaaaagct catcagcaaa
    16681 ttgttgtgct ccagcaaaaa cttcgccggg aacgacatat acgcccataa aagtgagttg
    16741 ttgtagtcca gcggctaaag ccattgagaa ccccatagtt acctgttgca gtaccaaagc
    16801 tgtagtaagt attaatccca taaaactagc caccaaagat gcgttgcaac agtttaaaga
    16861 tgtactgcca tatcttttgt taggagtatt aataggatct tttatttatg gctttattcc
    16921 ctcagcgtgg attgccgctc atgcaggagc agataatccc ttcgccatcc cattaagcgc
    16981 cgttgtcggt attccgctgt atatccgggc tgaggcagtt atccctctgg catctgttct
    17041 gatgacaaaa ggaatgggtc tgggagcatt aatggcatta atcatcggca gtgccggcgc
    17101 aagcctgacg gaagtgatat tacttaaatc aatgttcaga atgccgatga tagctgcatt
    17161 cctgacggtt atattaggta tggctatctt gatgggctat ttgactcaat tgctatttta
    17221 aatagatgcc agcagtaaac tataagatgc atagttagtg ccatatcctt ctaagtagaa
    17281 aatcatagac aggataatcc ttaaaaatca caaatgattt aactaatgca ttgagaatga
    17341 tgacggctcg gtcaacaagt catcaacaga caataaccac gtaatcatgc ttctctgaca
    17401 ttttatcgga gaagcaatga ctctttgtct cagtaccctc ctccttttta aaaggcagat
    17461 ttcagtcgct aatacaacac atttttaaat acactttaac ctggttcatt attacacatc
    17521 taaaacatca caaaaatatt acttcacaca ctatcggaga acataataac tttttaaaat
    17581 gagcgctttt tgatagttgt taacgttttc ctttatgata ct
//
LOCUS       DAZGAN020000101        17554 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGAN020000101 DAZGAN020000000
VERSION     DAZGAN020000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.101"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..84)
                     /locus_tag="ACHKYO_RS18615"
                     /old_locus_tag="pgaptmp_003723"
     CDS             complement(<1..84)
                     /locus_tag="ACHKYO_RS18615"
                     /old_locus_tag="pgaptmp_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 domain-containing protein"
                     /protein_id="HGU4430494.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESA"
     gene            complement(210..941)
                     /gene="rlmB"
                     /locus_tag="ACHKYO_RS18620"
                     /old_locus_tag="pgaptmp_003724"
     CDS             complement(210..941)
                     /gene="rlmB"
                     /locus_tag="ACHKYO_RS18620"
                     /old_locus_tag="pgaptmp_003724"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGU4430495.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1121..3562)
                     /gene="rnr"
                     /locus_tag="ACHKYO_RS18625"
                     /old_locus_tag="pgaptmp_003725"
     CDS             complement(1121..3562)
                     /gene="rnr"
                     /locus_tag="ACHKYO_RS18625"
                     /old_locus_tag="pgaptmp_003725"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGU4430496.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3601..4026)
                     /gene="nsrR"
                     /locus_tag="ACHKYO_RS18630"
                     /old_locus_tag="pgaptmp_003726"
     CDS             complement(3601..4026)
                     /gene="nsrR"
                     /locus_tag="ACHKYO_RS18630"
                     /old_locus_tag="pgaptmp_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HGU4430497.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4231..5529)
                     /gene="purA"
                     /locus_tag="ACHKYO_RS18635"
                     /old_locus_tag="pgaptmp_003727"
     CDS             complement(4231..5529)
                     /gene="purA"
                     /locus_tag="ACHKYO_RS18635"
                     /old_locus_tag="pgaptmp_003727"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGU4430498.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5633..5830)
                     /gene="yjeT"
                     /locus_tag="ACHKYO_RS18640"
                     /old_locus_tag="pgaptmp_003728"
     CDS             complement(5633..5830)
                     /gene="yjeT"
                     /locus_tag="ACHKYO_RS18640"
                     /old_locus_tag="pgaptmp_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HGU4430499.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5912..6916)
                     /gene="hflC"
                     /locus_tag="ACHKYO_RS18645"
                     /old_locus_tag="pgaptmp_003729"
     CDS             complement(5912..6916)
                     /gene="hflC"
                     /locus_tag="ACHKYO_RS18645"
                     /old_locus_tag="pgaptmp_003729"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HGU4430500.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6919..8178)
                     /gene="hflK"
                     /locus_tag="ACHKYO_RS18650"
                     /old_locus_tag="pgaptmp_003730"
     CDS             complement(6919..8178)
                     /gene="hflK"
                     /locus_tag="ACHKYO_RS18650"
                     /old_locus_tag="pgaptmp_003730"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HGU4430501.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGNIMDQRRANAQRNDYQRQGE"
     gene            complement(8264..9544)
                     /gene="hflX"
                     /locus_tag="ACHKYO_RS18655"
                     /old_locus_tag="pgaptmp_003731"
     CDS             complement(8264..9544)
                     /gene="hflX"
                     /locus_tag="ACHKYO_RS18655"
                     /old_locus_tag="pgaptmp_003731"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HGU4430502.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9620..9928)
                     /gene="hfq"
                     /locus_tag="ACHKYO_RS18660"
                     /old_locus_tag="pgaptmp_003732"
     CDS             complement(9620..9928)
                     /gene="hfq"
                     /locus_tag="ACHKYO_RS18660"
                     /old_locus_tag="pgaptmp_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HGU4430503.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10014..10964)
                     /gene="miaA"
                     /locus_tag="ACHKYO_RS18665"
                     /old_locus_tag="pgaptmp_003733"
     CDS             complement(10014..10964)
                     /gene="miaA"
                     /locus_tag="ACHKYO_RS18665"
                     /old_locus_tag="pgaptmp_003733"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGU4430504.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10957..12804)
                     /gene="mutL"
                     /locus_tag="ACHKYO_RS18670"
                     /old_locus_tag="pgaptmp_003734"
     CDS             complement(10957..12804)
                     /gene="mutL"
                     /locus_tag="ACHKYO_RS18670"
                     /old_locus_tag="pgaptmp_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGU4430505.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12814..14151)
                     /gene="amiB"
                     /locus_tag="ACHKYO_RS18675"
                     /old_locus_tag="pgaptmp_003735"
     CDS             complement(12814..14151)
                     /gene="amiB"
                     /locus_tag="ACHKYO_RS18675"
                     /old_locus_tag="pgaptmp_003735"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HGU4430506.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14170..14631)
                     /gene="tsaE"
                     /locus_tag="ACHKYO_RS18680"
                     /old_locus_tag="pgaptmp_003736"
     CDS             complement(14170..14631)
                     /gene="tsaE"
                     /locus_tag="ACHKYO_RS18680"
                     /old_locus_tag="pgaptmp_003736"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGU4430507.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14603..16150)
                     /gene="nnr"
                     /locus_tag="ACHKYO_RS18685"
                     /old_locus_tag="pgaptmp_003737"
     CDS             complement(14603..16150)
                     /gene="nnr"
                     /locus_tag="ACHKYO_RS18685"
                     /old_locus_tag="pgaptmp_003737"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HGU4430508.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16149..17288
                     /gene="queG"
                     /locus_tag="ACHKYO_RS18690"
                     /old_locus_tag="pgaptmp_003738"
     CDS             16149..17288
                     /gene="queG"
                     /locus_tag="ACHKYO_RS18690"
                     /old_locus_tag="pgaptmp_003738"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HGU4430509.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLIGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17271..17324)
                     /locus_tag="ACHKYO_RS18695"
                     /old_locus_tag="pgaptmp_003739"
     CDS             complement(17271..17324)
                     /locus_tag="ACHKYO_RS18695"
                     /old_locus_tag="pgaptmp_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430510.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3986 a         4993 c         4400 g         4175 t
ORIGIN      
        1 tgcgctctcg ctatttgtca catcaatatt ttgttcaggt acagtttgca gcgccgtcgc
       61 cagcgccaac ggattccatg tcatatatta ttccatatag atttagttta aatattaata
      121 aaatgaatat ttgcagtacg taattatctt accagctata gacaaaaaaa accatccaaa
      181 tctggatggc ttttcataat tcagacgaac tagctgcgct gacgaaccgc ttcaaataag
      241 caaattccgg ttgcaaccga aacgttcagg gaagaaacac ttcctgccat tgggatgctg
      301 atcaactcat cgcaatgttc acgggtcagg cgacgcatac cttcaccttc cgcgcccatc
      361 accagcgcca ggcgtccggt cattttgctc tgatagagtg tatgatccgc ctcgcctgcc
      421 gtaccgacga tccagatatt ctcttcctgc aacatacgca tggtgcgcgc aaggttagtc
      481 acccgaatca gtggaacgct ttctgccgcg ccgcaggcta cttttttcgc cgtagcgttg
      541 agctgtgcgg agcgatcttt cggcacaatc accgcgtgaa cgccagcagc gtccgcgcta
      601 cgcaggcacg cgccgaggtt gtgcggatcg gttacaccgt cgaggatcag caggaacggt
      661 tgatcgagcg aagcgatcag atccggcaga tcgttttcct gatactgacg tcctggcttc
      721 acgcgggcga taatgccctg atgcacggca ccgtcgcttt tctcgtcgag atattggcgg
      781 tttgccaact ggataaccac gccctgagac tcaagggcgt gaatcagcgg taacagacgt
      841 ttatcttcac ggccttttaa aataaagact tcctgaaaac gttcaggggc gcgctccagc
      901 agggcctgca ctgcgtggat gccgtaaatc atttcgctca ttaatgtact cgttgtttac
      961 gttttgcctg atgcactacg tttatccggc ctacatgatc tctgcaatat attgaatttg
     1021 cgtgattatg taggccggat aaggcgttca cgccgcatcc ggcatgaaca aagcgcattt
     1081 tgtcagcaat ctaaccctct tcttttaaag agggtattga tcactctgcc acttttttct
     1141 tcgccgcacg cttcgctttg gtcgctgcgg ctattttctg cgttttcgcc gatggctttt
     1201 tcgcttttct cgcgtctttc ttcgccgctt tcggcttcgt ttttttctca ccgcggaagg
     1261 cgctgtctgg ctcaaagttt acctttttac cgacctgacg acgcttgccg ccttttttgc
     1321 ctgcatcgcc ttttttcgct ttctcgcgcg ccgttttacc gacgttgcgc ggtgcgcgtt
     1381 cactggagat cagactaaag tcgattttgc gctcgtccat attaaccgct tcgactcgaa
     1441 cttccacgcg atcgcccagg cgataagtct ggccgctgga ttcccccatc aggcgttgcc
     1501 ctacctggtc aaagcgatag tagtcattgt ccagcgaaga gacatggacc agaccatcaa
     1561 tgaacaagtc gtccagacgg acgaagaagc caaagccagt gacgctggaa attacgcctt
     1621 taaagacgtt acctacctgg tcgagcatga agtcacactt cagccagtca gcgacatcgc
     1681 gcgttgcttc gtcggcacga cgttccgcca tcgaacagtg ctgacccagt tgcaacatct
     1741 cttccatcga ataatggtag ccgccggttt cagtggtgtt gccctgatgc ccctgctctt
     1801 tcgccagcag atacttaatg gcgcggtgca gcgtcaggtc aggataacga cgaatcggcg
     1861 aagtaaagtg cgcataggac tgcaatgcca ggccaaagtg accacggttt tctggatcgt
     1921 aaatcgcctg tttcatcgag cgcagcagca tggtttgcag catttctgca tcaggacggt
     1981 cggcaaccga ctccagcagt tccgcgtagt cacgcggttc cggcttgtta ccacccggca
     2041 gctccagccc cagctccgcc agcactgaac ggaaagaggt aatcgcttcg gtgctcggct
     2101 tgtcgtgaat acggaacagt gccggttctt tcgctttctc aacgaaacgc gccgccgaga
     2161 tattcgccag aatcatgcac tcttcaatta atttgtgcgc gtcgttacgc tgggtctgtt
     2221 cgatacgttc aatacggcgt tcagcgttga aaatgaactt cgcttcttcg ctctcaaatg
     2281 agatcccacc gcgttcttca cgggctttat ccagcacttt atagaggtta tgcaactctt
     2341 cgagatgctt aaccagcggg gcgtactgct cgcgcagatc ctgatcgccc tgcagaatat
     2401 gccagacttt ggtgtaggtc agacgcgcgt gagagctcat caccgcttcg tagaatttgt
     2461 agcccgtcag gcggcctttc gacgaaaccg tcatctcgca caccatacac aggcggtcta
     2521 cctgcgggtt gagcgaacac aggccgttag agagcacttc cggcagcatc gggataacct
     2581 gcgaagggaa gtacaccgac gtgccacggt tacgcgcttc tctgtccagc ggcgttggcg
     2641 gacgcacata gtagctgacg tcggcaatcg cgacccataa acgccagccg ccgccgcgtt
     2701 ttttctcgca gtaaactgca tcgtcaaagt cacgggcgtc ttcgccatca atggtgacca
     2761 gcggtaaatc gcgcagatca acacggcccg cttttgcttc ttccggcact tcttctttca
     2821 gcccggcaac ctgttgctca acagcctgcg gccagatgta cggaatttca tgggtacgca
     2881 gagcgatatc aaccgccatg ccggtgccca tattgtcgcc cagcacttcg acgattttac
     2941 ccaccgcttt ggtgcggcga gtcggacgct gagtcagttc gactacgacc acaaagccca
     3001 tccgcgcgcc catgatctga tcgggcggga ttaagatatc gaagctcaga cggctatcgt
     3061 caggaaccac aaagccgacg cccgcttcag taaagtagcg accaacaatc tggctggttt
     3121 ttggcaccag tacgcggaca atacgcgctt cacgacgacc tttacggtca gcgcccagcg
     3181 gctgcgccag cacctgatcg ccatgaatgc aggttttcat ctgctcgctg gagagataca
     3241 aatcatcttt acgcccttca acccgcagaa agccgtagcc atcacggtgg ccaataacgg
     3301 tacctttcac caggtcgagg cgttccggca gcgcatagca ctgacgacga gtgaagacca
     3361 gttgaccatc gcgctccatc gcgcgcaggc ggcgacgcag gccttcaagc tgctcttcgc
     3421 cttcaatgtg cagttctacc gccagctcat cacggctggc cggtttttca cgtttggtta
     3481 aatgttcgag gataaattcc cgactaggga tgggattcgc gtatttttca gcttcgcgtt
     3541 cctggaaagg atcttgtgac atctcggttc ctccgttgtc atctctgatg aagattttcg
     3601 tcactccacc agcaataatt tataaagcgg ttgattctct tcaaccaaat cggcaagcgt
     3661 gtagttatcc agttccgtaa gaaaactttg cacggcctta gaaagcgcct gtttcaaccg
     3721 acaggcaggt gtaatgtggc aaaactcact gctgcaattc accagcgata agggctccag
     3781 ctcgcgcacc acatcaccaa tacgtatcgc actcgccgat ttacccaggc gaatgccgcc
     3841 attttttcca cgaacagcag tcacgtagcc ggcacgacta agttgattga ttattttgac
     3901 catatgatta cgggagacgc cgtagacgtc agtcacttca gaaatactgg tcatccgccc
     3961 ttctggcaat gacgccatgt agatcagcgc acgtaatccg taatcagtga aactcgttaa
     4021 ctgcacatca acctcaaaag ggaaatcggg aaaaaataca tttatattga tgataaacca
     4081 gccacaagct gtgtcgctaa tttatttcag ttcggggaag gaaaaaagcg aggatttaac
     4141 actgtgtcgg atagcgtaat ggcttaaccg acacagcaat gtatcacact gttagcccgg
     4201 caggcaaaat atctgccagg cgtaccagaa ttacgcgtcg aacggatcgc gcagaatcat
     4261 ggtttcagta cgatccggac cggtagagat gatatcgatc ggcacaccag tcagctcttc
     4321 aatacgcttg atgtagttca gcgccgcctg cggcagaccg ctacgatctt tcacgccgaa
     4381 ggtggattca gaccagcccg gcatggtttc gtaaatcggc tctacacctt tccagtcgtc
     4441 agctgccagc ggagtggtag tcacttcgcg accatccggc atacggtaag ccacgcagag
     4501 tttaacctct ttcaggccat ccagaacgtc cagtttagtc aggcagaagc cagacaggga
     4561 gttcagctgt accgcacgac gaacggcaac ggtgtccagc cagccggtac gacgacgacg
     4621 ccccgtagtt gcgccgaatt cgttaccctg cttgcagagg aactcgccag tttcatcaaa
     4681 cagttcggtc gggaacggac ctgcacctac acgagtggag taagctttga ggatacccag
     4741 aacgtaatca acataacgcg ggcccaggcc ggaaccggtc gccacgccac cagcagtggt
     4801 gttggaagaa gttacgtacg gataagtacc gtggtcgata tccagcagcg taccctgcgc
     4861 accttcaaac atgacgaaat cgccacgctg acgcgcctgg tcgagcaggt cagaaacgtc
     4921 aaccaccata gaagtcagga tgtcggcaac agccatcgta tcatccagaa ctttctggta
     4981 atcaaccgct tcagctttgt agtagttaac caactggaag ttgtgatatt ccatcacttc
     5041 tttcagtttt tcagcgaagg tttctttgtc gaaaaggtcg ccaacacgca gaccgcgacg
     5101 tgctacttta tcttcataag caggcccgat accacgaccg gtggtgccga tcgctttcgc
     5161 gccacgcgct ttctcacgcg cgttatccag cgcaacgtga taatcaagga tcagcggaca
     5221 tgcttcagac agcagcagac gctcacgaac ggggatgcca cggtcttcca gttctttcat
     5281 ctctttcatc agcgcggccg gagacagcac aacaccgtta ccgatgatgc tggttacatt
     5341 ctcgcggaga atacctgatg gaataagatg gagaacggtt ttttcaccgt tgattacgag
     5401 agtatggcct gcgttgtgac cgccctggta gcgtacaaca tatttagccc gttcagtcag
     5461 aagatcgacg atcttacctt taccttcgtc accccattgg gtgcccagta cgacgacgtt
     5521 gttacccatt tttcaaaatc accgtttgct taaaaatgga ttctaccatc gctttttcag
     5581 agttacagca ctttttgcac tcaaaaatga cctcgatcag tctgtttttt gttcagccaa
     5641 tcgttttcct caacatgtag tagaccacaa cgcccgcaac cacaagtcca ccgccaaaac
     5701 gacgtaaaat attatcgggc aaattggtca tcgcagagat catcttcttc catgccttcg
     5761 ggtaaagcat cggccctaaa ccttccagta ccaaaaccag ggcaagcgcc agccagattg
     5821 tcgaattcat tcattatcct tatagaaaaa gaaaaccacc gacatccctg aggatgcggt
     5881 ggctttattg acctgtaccg cagtcgttat attaacgcgt tgcggaagtc ggcgtcttca
     5941 tgtagcggaa gaaatcgcta tccgggctca tgaccatcac gtcctgattg ccagagaagc
     6001 tgttctcata agcacgcagg ctacggatga atgcgtagaa gtccggatct ttactgaatg
     6061 catcagcaaa cagtttggct gcttcggcat cgccttcacc acgcatgatg cggccctgac
     6121 gctcagcttc tgccagcgtt ctggtcactt catagtcggc agtcgcgcgc agtttttccg
     6181 cttcttcctg accttgtgaa cggtgacgac gcgctaccgc ttcacgctcg gcgcgcatac
     6241 ggttgtagat cgcttcagac acttcggtcg gcaggttgat ctgcttgata cgcacatcga
     6301 caacttcaat acccagcgcc gccatactgt tcgggttgat gaccggaact ttgcccttcg
     6361 tctcagccgt tacgcgctct gccgcttcgg caatggcgtt atccgctgcc ggggtagtaa
     6421 cttcatcttc tgtacccgca gaaccggagt tcagcgcgtc acgtacttcg agggtcagac
     6481 gaccacggga atcggtgacg atgtctttca cgtccaggcg accaatttca gaacgcagac
     6541 ggtcagagaa cttacgtttc aacagcactt ccgcttgcga aatgtcgcca ccacccgttg
     6601 ccaggtagta acggctgaaa tcgctgatgc gccatttgat gtaagaatcg acgatcaggt
     6661 ctttcttctc tttggtcaca aagcggtcgg cctggttgtc catggtctga atacgtgcgt
     6721 cgagcatttt caccgtttca atgaacggta tcttgaaatg cagacccggc tcataaacca
     6781 gaggtttgtt gtcatcgtca cgcagtacct taccaaaacg cagcgtaata ccgcgctcac
     6841 cttctttgac gacaaagaca gacatgtaaa gcactaccag cacgatgatg ataatcgcga
     6901 taactgactt acgcatcgtt attccccctg acgctggtag tcgttacgct gcgcgttggc
     6961 gcggcgttgg tccataatat tgccctgact ggtggacgac gtgttgcttg ctccactggt
     7021 tgtggaggaa gaggctggcg gcaaacgcag cagattgctg gcaccgttat cgctcttcgc
     7081 cgcaggggcg ttaccacctt tcagcatctg gtctaacggc agaaccatca ggttgccacc
     7141 tttatcgtta accagcactt tgcgggtgtt acccaacact ttttccatcg tctcgatata
     7201 cagacgctcg cgagtaattt ccggcgcggc tttatattcc ggcagaagtt tagcaaagcg
     7261 cgccacttca ccctgagctt ccaggatggt ctgggccttg tacgcacgcg cctcttcgag
     7321 gatacgttgc gcctgaccgt tcgcacgcgg ctgaacttcg ttggtatacg cttctgcttc
     7381 acgaatgtat tgctgttcgt tttcacgcgc ggcaatcgca tcgtcaaacg ccgcttttac
     7441 ttcttccggc ggacgagcag cctggaagtt gacgtccagc agcgtgatac ccatgtcata
     7501 cggacggata gtctcttcca gttcgcgctg agtatcgcta cgaatcacgg tacgaccttc
     7561 cgtcagaatg cggtccatgg tgtatttacc gataactcca cgcagggcgc tgtcggtagc
     7621 ctgacgcagg ctgtcatccg ggctggtcac gctatacaga tatttttccg gattggtgac
     7681 gcggtactgc acgttcatct caacgcgcac tacgttctcg tccgacgtca gcatcacacc
     7741 agaagcggcc agttcacgca cggcttccac gttcaccggt ttgacttcgt cgataaacgt
     7801 cggtttccag ttcagacccg gctcaaccag atggctgaat ttaccaaagc gtgttaccac
     7861 gccgcgttcg gcttctttaa tggtatagaa accactggcc gcccagataa tgacaatcgc
     7921 tgccgctgcg atggtaacga cacgaccgcc aagctgcggg cgcgggcctt gcgatgaact
     7981 gccaccgcca gatccggtgc ctttaccgcc gcccagacca ccgagctttt tgctcagttt
     8041 gcggaagata tcatctaaat caggtggccc ttgatcgcga ccgcctttgt ttccatttcc
     8101 ctcagagttg ccgccaggtt tgctgcttcc ccacgggtcg cggtcttgtc cgttattacc
     8161 gggctgattc cacgccatgt ttgtgctcca tatttgttat gcggtgatcc ctgttgtctt
     8221 tcgacgccag aggatatgac tccacgcttc agacgctacg ccgttagatc aggtaatcga
     8281 tcaacgccgg ttcttgttta cagaggcgac gccagtcaac gatcggcata cgaacttgca
     8341 gacttacgct gccgtcctcc tccatccact ctttttctat tgcctgaagc tgataaaaac
     8401 gacttctcag acgcccttcc tgcggtggca gacgcaatgt atgctgcgcc acctcgccgg
     8461 aaagccgctc cgtcaaagcc tgaaaaagct gtggtatccc cgctccggtc tgtgcggaaa
     8521 gccagacacg gatcggtttg ttctcttcgt cccgatcaat acgcggttcg aaatcttcca
     8581 gcatatcgat cttgttcatc accagcaggg ttgggatctc gtgagcgtcg atctcttcaa
     8641 gaaccgtatt caccgcttcg atgttttctt gtacacgcac atccgccgca tcaatgacgt
     8701 gcagcagtaa tgtggcttgc cgcgtctctt gtaacgtggc tttaaatgcc gccaccagat
     8761 cgtgcggcag gtggcgaata aaccctacgg tatctgcaag tacggtttca ccgacatctg
     8821 caacgtcaat acgccgcaac gtcgggtcga gggtggcaaa caactggtct gccgcgtaga
     8881 cccgcgcttc ggtgatgcga ttgaaaaggg tagatttacc ggcgttggta tatcccacca
     8941 gcgaaacagt aggaacgtcg gctttgatac gcgattgccg cccctgctca cgctgctttt
     9001 caactctttc caggcgtgac tgtatctgca cgatgcgatt acgcaacaaa cgacggtcgg
     9061 tttcgagctg ggtttcaccc ggaccacgca aacctatccc gcctttctgt ctttcaaggt
     9121 gggtccagcc acgcaccagg cgcgtagcca gatggcgcag ctgcgccagc tcaacctgca
     9181 acttaccctc atgggtacgc gcacgttggg cgaaaatatc taaaataagg ccggtgcggt
     9241 cgataacacg acactcgcac aaacgctcca ggttacgctc ttgcgccggg ctcagggcat
     9301 ggtcaaaaag aacgaccgaa gcacccgtcg ctttgacagc ttccgcaatt tcaactgctt
     9361 taccttcacc tacaaaatac tttgggtgcg gcgctttacg gctaccggta atcacctgca
     9421 atgcttcgac accggcggaa gagaccagag attcaaactc ctggaggtct tccatatctt
     9481 tgtcttgcgt aaaatagatg tgtaccagta ccgcctgctc accagcatca taacggtcaa
     9541 acaagcgtat aaccctctaa atagatcagc ggggaacgca ggatcgctgg ctccccgtgt
     9601 aaaaaaacag cccgaaacct tattcggttt cttcgctgtc ctgttgcgcg gaagtattct
     9661 gcgcgctgct accatgatgg tagttactgc tggtaccgcc accggcgttg ttactgtgat
     9721 gagaaaccgg gcgagacggg acaacagtag aaatcgcgtg cttgtaaacc atctggctga
     9781 ccgtgttttt caacaggatc acgaactgat caaaagactc gatttgccct tgcagcttaa
     9841 taccattcac caaataaata gaaactggaa cacgttcccg acgcagtgcg ttcaggaacg
     9901 gatcttgtaa agattgcccc ttagccattc tctcttttcc ttatatgctt atttgtactt
     9961 tgaacctttc gattctgaaa aaattgcgca cgatacgtct caattgtaca cattcagcct
    10021 gcgatagcac caacaacctg taatacttcg tcacgcgcct gttctggttt ttcactgtca
    10081 agccagtgaa ccccttccca accacgcagc caggttatct gccgcttcgc caactgtctc
    10141 gtggcgcaaa cacctcgata aaccatttca tcgtatgaga tttcgccttc aaggtaagac
    10201 cacatctggc gataacccac gcaacgaatg gaaggcaagt ccgtatgcaa atctcctcgg
    10261 gcaaaaagcg cccggacttc tgcttcaaaa cctgaagcca acatctgatg aaaacgctgc
    10321 tcaatgcgtt gatggagcag ttcacggctc gccggggcga tggcgaactg atgcacctga
    10381 tacggtagag cgtctcctga cgtttgcgtc agttccgtta aagttttacc cgaaatgaaa
    10441 aaaacttcca gtgcccggga aagcctttgt ggatcatttg gatgaatcct tgctgccgca
    10501 accggatcta cctcctgaag ttgacgatgc aatgactccc aaccttgctc tgccgcctgt
    10561 tgctcaattc tggcccgcac ttccgggtct gccgacggta gcggcgacaa cccttccagc
    10621 aatgccttga aatacaacat cgtaccgccc actaacagtg ggatccgccc cgccgcggtg
    10681 atatcggcca tttccgccag cgcatcgcgg cgaaaatcag cagccgagta agcctgcgac
    10741 ggatcgcgaa tatccagcaa tcggtgcggc gcggcgagta actcttcagc gttcggcttc
    10801 gccgtcccga tatccatccc tttgtaaata agggcagaat caacgcttat caactctact
    10861 ggtaaaattt tacgcagctc aatggctaac gccgttttac cggaggccgt cggccccatc
    10921 aaaaaaatcg ccttaggcag gctcgcctta ctgatatcac tcatctttca gggcttttat
    10981 cgccggatgt aaatcaacag attgtaacag accacccggc ggcgttttca caagttgcgg
    11041 acataaccgt tccacgtccg ccagcagggt tatggcctgt gccattgacc actgcgcatg
    11101 ttcgctcatc agatttcgtg caatccactg cgcaatattg ccaggttcga atacggactg
    11161 cttcgccagg tagcctatca gttcaggaat caagatttgt aaattttgtt ggcgtaaggg
    11221 taaaggcacg gccctgatgg tcacatgctg tgcatctgac tggaaatcaa tacccaattc
    11281 cgccagggca gactgcgctt tttctaatgc cgatttttct tcggcagaaa cttttagccg
    11341 caacggaatc agcagcggct gggcgcaaac gggcgcttca cccggcgtca attgtgcctg
    11401 acgtagccaa cgttctgcca ctggcaagga taaaagtgaa atgttgccat cgcgctccag
    11461 caacgcacag tcggaatgga cgatagtcag tacccgacca aaactctgac tgttcgccgc
    11521 aagtgcaggt tcctgcggtt ccggcgcttt tggtttttgc atcggcgcgg gcgtttgcaa
    11581 aagctggcga tacacttcac cttgctgttt ctggtagcct ggctgcgcat tcggccaggg
    11641 ggcagccgga cgactgcctg atgctggcgc aggagtgtag cgcggagcta ccggctcacg
    11701 agctgccggt tctgcaaagt gattgcgccc cgccgccacg cggttttccg gaatggaacg
    11761 cggtgcaggt tggggttcat cgtccagcgg tagcggcgtt tccagttgct gttgtagcac
    11821 gctcagcacg ccctgataga taaaatcatg caccagacgc gactgatgga aacgcacttc
    11881 gtgtttggcg gggtgcacgt tgacgtccac ctgatgcgga tcgatctcca gatacagcac
    11941 aaatgccggt tgctgatcgg cccccagttt gtcttcgcag gcctggcgga tcgcgtgatt
    12001 gatcaggcga tcgcgcatca tgcgaccgtt cacgtagcaa tactgaattt ctgccagtgc
    12061 gggcgtggtg tgatttggat cggccaccca gccgcgtagc gtgagatcgc cgtgttgcca
    12121 ttcaatcgcc agcgcttgtt caagaaaagc ggtgccgcaa atcgcgccta agcgccgttc
    12181 tttttgcccg ccttccggca ctgcgcggta ctgacgcaca attttaccgt tatgcgacag
    12241 gttgatcgtg acgtcgaaac gcgccagcgc aatgcggcgg atgatctcat caatgtggtt
    12301 aaattcggtt ttctcggtgc gcaggaattt gcgccgcgcc ggggtgttgt agaacagatc
    12361 cagcacctcc agcgtcgtcc ccacaggatg cgccgccggt tttaccgtca cgttcatatc
    12421 gcgcccttcg gcataggcct gccaggcttc ctgctgttct gcggtgcgtg aagtgagcgt
    12481 caggcgggaa accgaactga tactcgccag cgcctcaccg cgaaagccca ggctgataat
    12541 ggcttcgaga tcgtccagag aggcgatttt actggtggca tgacgcgcca gcgccagcgc
    12601 cagctcgtct tttttgatac cgcagccgtt atcacgaatg cggataagtt tcgccccacc
    12661 gcgttcgata tcaatatcga tacgtgtcgc acctgcatcg aggctgtttt ccaccagttc
    12721 tttgactacc gacgcaggtc gctcgaccac ctcacctgcg gcaatctggt tcgccagttg
    12781 tggcggtaag acctgaattg gcatcaatcg tccttagttt ggcagcgtgc gatctggcgt
    12841 cgtcaccgta ctggccgttt gtgccgttgc cccctgcggc gcagattgca tcggatgcgc
    12901 aaggaaataa ttgcgcaggc ctttgtaaat ggcttctgcc agctgttgtt ggtaatcgtc
    12961 gctcgccagc aaacgttctt cgctgttgtt gctgataaaa ccggtttcga ccagtactga
    13021 tgggatatcc ggcgaacgca gaacgccgag actcgcgtgt tctggtcggc gtttatgtat
    13081 ttcgccaatg cgttgcaact gactgatcat actggtcgct acatcatacc ctacccgttg
    13141 ggaatgaccg aactgtaaat ccagcaccgc ctggcttaaa taggggtcag actgactgtt
    13201 cgccagcaca tcacccgccc cacccagcag ctccgactgt ttctcgtgct gctccagcca
    13261 gctggccatt tcgctgttgg cgcgacggtt agagagcacc catacggaag cgccagtcgc
    13321 actgcggttc ggtgcggcat cagcgtgaat cgacacgagg aaattggcgt tttgcttacg
    13381 tgccacatca ctgcgcccca tcaccgagat aaagtaatcc ccgtcacggg ttaaaacgcc
    13441 cttaaacatc ggatcgtcat tgagcaaagt acgcaattta cgcgcgatgg cgatggtgac
    13501 atttttctcc cgcgtaccac cggggccgat agcgccaggg tcctgaccgc cgtgtccggc
    13561 atcaatagcg atgataattt tatcgccagt gttagccgtc gcgcgcgctg ccggacgcgt
    13621 taccgtatta ctgctgataa cacccgtagt gcggttactt tccgttttaa acggattgcg
    13681 cgccggttcg ctgacgcgcg gtgcgacaac cgcaggcgtt tcaacgcgtt tcgcaaccac
    13741 gggcggcgga ggaggaggtg gcggcgcatc ggcgttaatc gtaaagacga cagtgtaatt
    13801 gctgccattc tgccgcttca ccgcttcggt tttaccgttt tcggtaagat cgaccaccag
    13861 ccgtagcgtt tgtgcatctt taggcgttcc agagcgaata gccttcacaa gattattgcc
    13921 gctgaacaac aacggcagtc cctgaatcac gcccgtttgt ttgatatcga gcgccacggt
    13981 gcgtttgctt tgatggctaa acgcataatc aggatcgcca ataaaactca acgttatccg
    14041 cgcctgttga ttaccgttag aaacctgaat atcagagagc gtcgcggcac ccaccggcgt
    14101 gcacagcagc agcagcgtcg ctaccaacca atttctgatg cgatacatca tcccgccacc
    14161 tttcaaaggt taaccggcta aacgcgccag caacaattca cccgcagagg aaaccgcact
    14221 cacgcgcgcc tcacggcctt gtgcctgata atcaatgtgt atttcgacat ccgggtcagg
    14281 aagaacacca gtaccttgtt gtggccactc caccaggcag atggcatcgt tggcaaaata
    14341 atcgcgaatc cccataaact ccagctcctc gggatcggca aggcggtaca aatcaaagtg
    14401 ataaaccatt aaattgtcga gcgtataggg ttcgaccagc gtataagtgg ggcttttgac
    14461 attaccctga tgacccagag cttgtaaaaa gccccggcta aaggtggttt tacctgcgcc
    14521 taaatcgcca tacagataga ttacggttgc gccatcgcag gctttcgcta cccgctcgcc
    14581 caggtctaat gttgcctgct catcagggag cggaattact cgattcatca tggtttttat
    14641 cagtcacttc cgggttaaca atacgctgta gcgtggaaaa gagatcggtt gccagcatcc
    14701 cgcgcgttcc aaaacgcgcc gccagtacgt cagctgcagc accgtgcgcg acacagcccg
    14761 cacaggcggc atcatacggc gacagttttt gcccaagcaa tgcgccaata ataccagaga
    14821 gcacatcgcc catgccgccg ctcgccatgc ctgcatttcc ggcatcaata atgcctaaag
    14881 cgtcaggatg ggcggcgacc acggttccgg cacctttcag caccgctacg ccgccataac
    14941 gttgtaccag acgtttggcg caatgtaagc ggtcactttc aatttcagcg acggaacagc
    15001 ctaacaaccg tgcggcctcg ccaggatgcg gcgtgatcac gcgattgtga cgcttatcgg
    15061 gattgattgc cagcaggttc aatgcatcgg catcccacaa catcggtttg cgaaaattct
    15121 caactttttg cagtgctttt ttcccccact cttgctggcc cagaccggga ccaatcacca
    15181 ccacatcggc ccattccagg ctttcggtaa gagagtccat cgtcagttca tgcaccatca
    15241 attccggtcg tgcagtcagc agcggcgcaa tgttttcact gcgggtcagt actcggacta
    15301 aaccagcacc agcacgtagc gccgcttccc ccgtcatacg aatagccccc gccgtgccgt
    15361 gatcgccacc gataattacc agccgcccgt gatcgccttt atgcgaagtc gggcgacgcg
    15421 gttttagcca gtgagaaagt tgttctgccg aaaaccgctg aattttcgtc tcctgacctg
    15481 ccagccaact atccagcccc agtgagtcaa aatgcagttg tccggtaaca tcccgcgctt
    15541 ttccagtgag caagcctggt ttcagcgcaa taaaagtgat ggtgtgatcg gcgttgatca
    15601 ctgcgcctgg cgtagcgccg gtttcagcca gcaggccgga agggatatca accgccgcaa
    15661 tcggcgcagg atgggaatta gcgtggtcga ttaactggct aatggattcg cggggcgctt
    15721 gctgcaagcc ggtaccgagc agcgcatcaa caatcagatc taccgattcg ggccagacaa
    15781 tattcgaagc atggatctcg cctcccgcgt ttaaccatgc ttcgcgtgcc agcgcggcct
    15841 cttccggcaa cggtttgtcg ctctcctggg ccagcagcgt gacctcaatg ccgaccgctg
    15901 tggccagccg cgcgaccacg tagccatcgc cgccgttatt accatgaccg cacagcacca
    15961 gccaatggcg ggcgtcagga tacgccgaac gacacacctg gaatgcggct tcgccagcgc
    16021 gaagcatcag ctcatagagt gtaagcccca gcgcatctgc cgcctcacgt tctccgcggc
    16081 ggatatcgtc ggcgtgccag acggtgtgtg gtatacttac ggggtttttc ttcattgtat
    16141 ggtccgtcat gtcagagccc ctcgatctca atcagttagc gcaaaaaatt aaacagtggg
    16201 ggctggaact gggctttcag caggtaggta ttaccgatac cgatctcagc gagtccgagc
    16261 ccaaactgca agcatggctg gacaaacaat accacggcga aatggactgg atggcacgtc
    16321 acggtatgct gcgcgctcgc ccccatgagt tattgcccgg tacgctgcgc gtgatcagcg
    16381 tgcggatgaa ttaccttcct gctaacgccg catttgccag cacgctgaaa aaccccaaac
    16441 tcggctatgt tagccgttat gcgctgggcc gtgactatca caaacttctg cgcaaccgac
    16501 tcaaaaagct gggcgagatg attcagcaac attgtgtttc gctgaatttt agaccgtttg
    16561 tcgattctgc gcctattctc gagcgcccgt tagctgaaaa agctgggctc ggctggacag
    16621 gtaagcactc acttatcctc aatcgcgagg ccggttcgtt cttcttttta ggcgaattgc
    16681 tggtcgatat tccgctgccc gtggatcaac cagtcgagga aggatgcggc aaatgcgtgg
    16741 cctgtatgac gatttgcccg accggtgcca tcgtcgagcc atataccgtc gatgctcgcc
    16801 gctgtatctc ttatctcacc atcgaactgg aaggggcgat cccggaagag ttgcgaccgt
    16861 taataggaaa ccgtatttac ggttgcgatg actgccagct tatctgcccg tggaatcgct
    16921 attcacaact caccacagaa gaggatttca gcccgcgtaa gccgctacac gcaccggaac
    16981 tcattgagtt attcgcctgg agcgaagaga agtttttaaa agtcacggaa ggatcggcga
    17041 ttcgtcgtat tggtcacctg cgttggctgc gtaatatcgc cgtagcatta ggcaatgccc
    17101 cttgggatga aacgattttg acagcgctgg aaagtcgtaa aggtgagcac ccacttctcg
    17161 atgagcacat agcgtgggcg attgcgcagc aaatagagag acgaaatgcg tgcatagtcg
    17221 aagtgcaatt gccgaaaaaa cagcgtctgg ttcgggtgat tgaaaaaggg ttaccgcgtg
    17281 acgcctgatt catccacagc ttgtgaataa aaataaaaac gcattgcaat tcaagacgaa
    17341 taaaatcgtc aagtgatcga ctcgacaaaa cggaatctta atttttaact tatctatcaa
    17401 taggttaaaa aaatactatt caccgtgcga agtttctttg aaattaagat tacagcctga
    17461 ggctgtggat aactctgttt acaagagttt gtcagagaca acgaaaacca gcaataacgc
    17521 atcgcatcgc tgtggatatt ttattgagag aaga
//
LOCUS       DAZGAN020000102        17531 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGAN020000102 DAZGAN020000000
VERSION     DAZGAN020000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.102"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            550..2241
                     /locus_tag="ACHKYO_RS18700"
                     /old_locus_tag="pgaptmp_003740"
     CDS             550..2241
                     /locus_tag="ACHKYO_RS18700"
                     /old_locus_tag="pgaptmp_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HGU4430511.1"
                     /translation="MVEEDEKNVPGDTCYYRYEIIGDSTHRTSIHNSFKTIQCRRVPV
                     RGITLTVGVFFDGTGNNRANADDLRLAYAHCANLVDEERARACEKYEKRARAGLGNAS
                     WQGGHTNISRLFDLYQQSDELNDSETEAQVKAYISGIGTADGESDSILGKALGSSLIR
                     QFEGVVTKTDEALEKISAELTRFIKLNRDKVAIAKVQFDVFGFSRGAAAARHFANRVM
                     NQDAAIAAAIDKGTDLTGHHGKLAGEVRFLGLFDTVAAIGSLLNFYDVNGRSNPGVNL
                     ELRPSVAQKVFQISAMHECRYNFSLNSIAGMWPELALPGAHSDIGGGYNAGEDEISLL
                     TMPDFEVTRDSADEMQTEVYRKAEKMRQVLYSLPALKYLMPHGEVETKMISWPLVNQN
                     KARASIFEKKVGAAVFMTRRDVPNDWEKVSLRVMLDAAQDAGAVFTPILSSNKELALP
                     SELLPLCDKAIAQGRAVRRGAEPEGFTAEDMRTIGRYLHCSANWNIDSDLSLWVSPSS
                     GEVFLRNNYAPTDERSFVWPMAPGKGWVRTVWRMDDQQQWEERARVNADALDGLF"
     gene            2252..2956
                     /locus_tag="ACHKYO_RS18705"
                     /old_locus_tag="pgaptmp_003741"
     CDS             2252..2956
                     /locus_tag="ACHKYO_RS18705"
                     /old_locus_tag="pgaptmp_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HGU4430512.1"
                     /translation="MRRLPLMVGVIVGVMLAVGCHDRRAGVKTLLGDGMDQWGQKLPY
                     DHWQFNFFYPKNLPALVTMVYLEDGDVRESIFRQLDAARPSQSSVDTWSQRVGSFSAS
                     FNIGKALPVRMTVCWDSVIDKKAYETEIWFSRDTWQQMLTAYPDTYKPGKTYYRDNMI
                     IGLAPGGAVRIWLEDNGEPVVLQHPARQFTLTGDDMLICKNVPNQIDFSYIKANGYDP
                     FIRDFIKEKPYPYGHW"
     gene            complement(3200..3313)
                     /locus_tag="ACHKYO_RS18710"
                     /old_locus_tag="pgaptmp_003742"
     CDS             complement(3200..3313)
                     /locus_tag="ACHKYO_RS18710"
                     /old_locus_tag="pgaptmp_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HGU4430513.1"
                     /translation="MHDPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
     gene            3621..4082
                     /locus_tag="ACHKYO_RS18715"
                     /old_locus_tag="pgaptmp_003743"
                     /pseudo
     CDS             3621..4082
                     /locus_tag="ACHKYO_RS18715"
                     /old_locus_tag="pgaptmp_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4926..9260
                     /locus_tag="ACHKYO_RS18720"
                     /old_locus_tag="pgaptmp_003744"
     CDS             4926..9260
                     /locus_tag="ACHKYO_RS18720"
                     /old_locus_tag="pgaptmp_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU4430514.1"
                     /translation="MNLRKKHICIAISLVLNPASISQASAQADTNKLTVEVDETSLGH
                     DVYGDDWKSIEIGTTKFTAKDESKGINFDHKSPVAEGRNSIAIGTSAEAGGDISTKEG
                     LYTGESIAIGAKTKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGKTYGREKIENN
                     YLASEAKGDGSIALGSRTFAKGELSVSLGSVSSAEGMASNAIGATAEAKGDMATAVGF
                     NALANAGNALAVGSQSSANKNDALAVGSEANAAQNETVALGHGATVTVAKGNVSIGAD
                     SRDKQAQKVTEAKVRTKRGAEVTYSGFAGNNAASVVSVGDAGKERQIVNVGAGAISDT
                     STDAINGSQLYAIAKTLKDDLDAINPTWTLKDDKGGSATINGGKDNKNGNTLTVKGDG
                     DIAVTVNDKGMTIDGTKLTENIKTDAIKIKDNNGGLLKKDLSDEIEITGKGPIKTQVA
                     GDKLEISAETASLPVNGGNGKITVSNGDDDKLVTAKNIAEAINQSGWKIKADKDTGGV
                     IEGNAQGTETLIKPGDMVTLKAGKNLSVKQSNGDFTFSVIDTPSFSSVQLNGNGKNGN
                     VTLSSEGNKLTLSGGNGAGQNGVVISGVASGLNNKDLAGLKADDAEMKNAASVADLKT
                     AIDGVSTSMQGAGFALKDSKGQEVKQSLGQAIKVSGDSNITTTAITANGDKGLKISLN
                     NDLTVGDKAPGSIKVNGADGKEAVSLNGQDGTIKLTSSPAAGGKSASATIAVNAGKGG
                     LTDAAGTTKTRLTYAPDGTLPAEELATMNDGLSFEGDDGKIINKKLNEKLTIKGGAEK
                     NAEVTDGNIRVDNDNGGLLVRMTRNLRDLASAVFGTENGPTTRIDNAGITITTPAAGG
                     GQGNTVSLTGSGLNNGGNQITSVASGLNGTALADAGGDVLNNAANIGDLKNALRDSSA
                     ALVNTGFGLQADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLTVRLADNIRLGDDGS
                     VSVGGTRMDKNGLTIKDGPSVTVNGIDAAGRVISGVKDGVNDQDAVNVSQLNREISQA
                     TAATTWALKTESGDEAVAVSSQTVEVRHGQNTRVSAISKDDKGNYSYEIDVTGIPVEY
                     TDSQGNPLVNIGGRFYTQTEDAATGKLTMTPAEPARVRISSEQPLVLTNVAAGNVSPV
                     STDAVNGSQLASAARIAGGDNVVWKDGKAALAPDTFSELTTAGGGKTTVNGGVHKTPD
                     NVADAVNMLNREGTKYFKAASSGQAAKAEGRDSIAVGQGAVSRGQNSIAMGNGAEVTR
                     QAAAGSVAVGSHARAGRANTGTYALNGQAVAGRTGSDTAVVSFGQPGQERQLQSVAPG
                     VLSANSTDAVNGSQLHATNRQVAGNTQAINTLGNKFSQLSYRVEELNRDIRGVGASAA
                     AMSGIPQAYLPGKSLMGLGVGGYGGESAIAIGVSRISDNGKMIMKLNAGQNTRGNFSV
                     GAGVGWQW"
     gene            9285..10058
                     /locus_tag="ACHKYO_RS18725"
                     /old_locus_tag="pgaptmp_003745"
     CDS             9285..10058
                     /locus_tag="ACHKYO_RS18725"
                     /old_locus_tag="pgaptmp_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU4430515.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTLTERGRD
                     ALSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRGWFIARGFRAERIH
                     ATGRGYDAPVVFCPDVEGEALKECLRPNRRVEITGQSVR"
     gene            10375..10890
                     /gene="ehxC"
                     /locus_tag="ACHKYO_RS18730"
                     /old_locus_tag="pgaptmp_003746"
                     /gene_synonym="hlyC"
     CDS             10375..10890
                     /gene="ehxC"
                     /locus_tag="ACHKYO_RS18730"
                     /old_locus_tag="pgaptmp_003746"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HGU4430516.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWLSGERKWFIDWIAPFGHNMEL
                     YKHMRKKYPYELFRAIRLDEYSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            10892..13888
                     /gene="ehxA"
                     /locus_tag="ACHKYO_RS18735"
                     /old_locus_tag="pgaptmp_003747"
     CDS             10892..13888
                     /gene="ehxA"
                     /locus_tag="ACHKYO_RS18735"
                     /old_locus_tag="pgaptmp_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HGU4430517.1"
                     /translation="MTVNKIKNIFNNATSTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADDLGIEIHRTERDDTAIANQFFGTAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PNLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAIADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVADKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFVTPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSNLIQFVEKDNKHYQARIISELGDKDDIVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDRDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKADKIPHNNNERSGYIKASNIASEKNMVNITSVADDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            13938..16058
                     /gene="ehxB"
                     /locus_tag="ACHKYO_RS18740"
                     /old_locus_tag="pgaptmp_003748"
                     /gene_synonym="hlyB"
     CDS             13938..16058
                     /gene="ehxB"
                     /locus_tag="ACHKYO_RS18740"
                     /old_locus_tag="pgaptmp_003748"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HGU4430518.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            16062..17501
                     /gene="ehxD"
                     /locus_tag="ACHKYO_RS18745"
                     /old_locus_tag="pgaptmp_003749"
     CDS             16062..17501
                     /gene="ehxD"
                     /locus_tag="ACHKYO_RS18745"
                     /old_locus_tag="pgaptmp_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HGU4430519.1"
                     /translation="MRFYMKGLWDLICRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVIWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGAFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESFRER"
BASE COUNT         5086 a         3240 c         4614 g         4591 t
ORIGIN      
        1 tttaaatcat gttgttatga tttgaaaaat atattttttt attcagattt atacaaaagc
       61 gagttgtcgg cgactactgg atgattcggc aacgagtcgt cggtgattta tcgatgactc
      121 aacacactgt ctctctggtg ttcagaaaaa gcgagtcgcc agagagtcgc gaggaaaaat
      181 actggatgac tcgctgatga ctcccggggt gaatcgctca cgactcgtct gtaactactg
      241 tctgatttct tctcatcagg gctgaaaacg tccgaaaatg cgccttatgg gcatcaggcg
      301 cgcccaaacc gatcgccatt cagtttcatg catagtcatg cagtgccatg aaaacaatcc
      361 tgacgcattt ttccggttta cccgggggaa aacatctatt tttgcggttt ttgcgccaga
      421 atcgcgctga gcgcgtttct gcggtgcgta caattaaggg attatggtaa atacgtctaa
      481 caggcagatg taaaaaagtc ccgaaggatt ttgctactga ttttatcttt cagataaata
      541 aggattttta tggtcgaaga agatgaaaaa aatgtgccgg gcgatacctg ttattaccgg
      601 tatgaaatta ttggggacag tacccatcga accagcatac acaacagttt taaaaccatc
      661 cagtgtcggc gggtgccggt cagggggata acgctgacgg tgggggtgtt ctttgacggg
      721 acggggaata accgggcgaa cgcagacgat ctacggctgg catatgccca ctgtgccaat
      781 ctggttgacg aggagcgggc tagggcatgt gagaagtatg aaaagcgcgc cagagctggg
      841 ctgggtaatg caagctggca gggcggccac actaatattt cccggctgtt cgatctttat
      901 cagcaaagcg atgaacttaa cgacagcgag actgaggcgc aggtcaaagc ctatatcagc
      961 ggcatcggga cggcagacgg agaaagtgat tcgatactgg gtaaagcgct gggcagcagc
     1021 cttatccgac agtttgaagg ggtggtgaca aagaccgatg aagcactgga gaagatcagc
     1081 gcagaactga cccgctttat caaacttaat cgtgacaaag tggcgattgc gaaggtgcag
     1141 tttgacgtgt tcggattcag ccgcggcgcg gcggctgcac ggcattttgc caaccgggtg
     1201 atgaaccagg atgcggcgat agcggcggca atagacaaag ggacagacct gaccgggcac
     1261 cacgggaaac tggcgggaga ggtgcgcttt ttagggctgt ttgacacagt ggcggctatc
     1321 gggagcctgt tgaacttcta cgacgttaac ggacgcagca atcccggggt gaatctcgaa
     1381 ctgcgtcctt cagtggcgca gaaggtgttt cagattagcg cgatgcatga gtgtcggtat
     1441 aacttcagcc tgaacagcat cgcggggatg tggccggagc tggcgctgcc gggggcgcac
     1501 tcggacattg gcgggggcta taacgcagga gaggatgaaa tctccctgct gacgatgccg
     1561 gattttgagg tgacgcgcga cagcgcggat gaaatgcaga cggaggtgta ccgtaaggcg
     1621 gagaagatgc gtcaggtgct gtactcactg ccggcgctga agtacctgat gccgcacggt
     1681 gaggtggaga cgaaaatgat ttcgtggcca ctggtcaatc agaacaaggc gcgtgcgtct
     1741 atttttgaga agaaagtggg cgcggcagtg tttatgacgc gcagggatgt gccgaacgac
     1801 tgggagaagg tgagtttgcg ggtgatgctg gatgcggcac aagacgccgg ggctgtgttt
     1861 actccgatac tcagcagtaa caaagagctg gcgttgccgt cagaactgct tcccctgtgt
     1921 gacaaagcca tagctcaggg gcgtgctgtc cggcgtggcg cggagcctga gggcttcacg
     1981 gcggaggata tgcggaccat cgggcgttac ctgcattgct cggcgaactg gaacatcgac
     2041 tcagacttga gcctgtgggt gagtccgtcc tcgggagaag tttttctgcg aaataactat
     2101 gctccgactg acgaacgctc gttcgtgtgg ccgatggcac cgggcaaagg ctgggtgcgg
     2161 acggtgtggc ggatggacga ccagcagcag tgggaagagc gggcgcgggt aaacgcggat
     2221 gcgctggacg gactgttctg aggaggcagg aatgagacga ttaccgctga tggtgggcgt
     2281 gatagtgggc gtaatgctgg ctgtcggctg ccatgaccgt agggctggcg tgaagacgct
     2341 gttgggcgac ggtatggatc aatgggggca gaagctgcct tatgaccact ggcagtttaa
     2401 ttttttttac ccgaaaaacc tgccagcact ggtgacaatg gtgtatctgg aggacggtga
     2461 tgtacgggaa agcatatttc ggcagcttga tgcagccaga ccaagtcaga gtagtgtgga
     2521 tacatggagc cagcgcgtag gcagtttttc agccagtttt aacattggca aggcactgcc
     2581 ggtcaggatg acggtgtgtt gggattcggt tatcgataaa aaggcatatg agacggagat
     2641 ctggttcagc cgggatacgt ggcagcagat gttgactgcc tatcctgata cctataagcc
     2701 gggaaaaacg tattatcggg acaatatgat tatcggattg gcaccgggag gggccgtgcg
     2761 gatctggctg gaggataatg gcgagcctgt tgtactccaa catcctgccc ggcagttcac
     2821 tctgacggga gatgatatgc tgatatgcaa aaacgttccc aaccagattg atttcagtta
     2881 catcaaggct aatggttacg atccatttat ccgcgatttt atcaaggaaa agccctatcc
     2941 gtatggtcac tggtagggag caacgatcag caaaattaaa atgatcttct tgagatcctt
     3001 ttaagctgcg cgtaatcttc ggcttgtaaa cggaaaaagc cgccttgcgg cggctttttc
     3061 cgtccgatta gaagtcttcg acctcttcta atcgactgtg gtaactggct tggtggagcg
     3121 cagataccga atactgtcct ttcagtgtag ccgtagttgg gtgatcactt caagactcta
     3181 atatcggaat ccaccttgtt tagttaccaa tggctgctgc cagtggcgtt aagtcgtgtc
     3241 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3301 gggggtcgtg catacagccc agcttggagc gaacgaccta caccgaactg agataccaac
     3361 agcgtgagct atgagagggg tcggtttggg aaacggaatt attgtacgtt aggagcattt
     3421 tttaatcgat gcggataagt aactgattga ttcatcgtga tcagctatcg cctttaagtt
     3481 attgcttttc tcttattgca tttaggttat ctcatgtccc taaaggtctg ccaaataccg
     3541 tatagcgttg gagtacaccc taccgcaata gcgcaaccgt acaatattgt acgtttccca
     3601 aacgcagccc tatcgaggca ctgtacgcta tcgagtctga gatccgcggc agtccggcag
     3661 aagagcgact ggcggtcaga aaagccagaa cggtaccgct aatgcagtcg ttgtatgagt
     3721 ggctccaggg gcagatgagc acgctgtcgc gtcactcgga tacggcgaaa gcgttcacct
     3781 atctgctgaa gcaatgggac gccctgaacg aatactgccg caatggctgg gtggagatcg
     3841 acaataatct gtgtgaaaac gccctccggg tagttgcact ggggcggcgt aactacatgt
     3901 tcttcggctc tgatggagga ggcgagagtg cggcagtgat gtacagcctg atcggtagct
     3961 gcaaacttaa cggaatcgag ccggaaacgt ggctacgcca cgtgatcagt gtcatcaaca
     4021 cctggccagc caaccgcgtg aaagagttgt tgccctggaa cgtcactcta tctgtaaact
     4081 aatttttacc ccacgtcctt cacggtgcgc ttacggataa ttaatgtcac tctgtatgga
     4141 tgaaaaaact gacagcagaa gtctccgctg acaaagacag gaatgatgat taatgtcaac
     4201 ttggccagtg agccagcccc ttaaaacgcc agacagtgga tgtatattaa aataagagat
     4261 aggctgggaa aatataaaga gtaactgcgc tttctgagta aaaaaacaac agcagcaggt
     4321 acttcaaaac cgtgatgcac tgtcagttgc aaagacgaat gtacagttac ctttgttacc
     4381 agaggacaag aagtgccccg ggtattaccg tgaaagaaat cagtgaacat ttattgacca
     4441 taaccacatt gcttctccgt agactgttga tttgaaagag ttaacatccc cgtaaaataa
     4501 tcaggcctgt atgaacctga ttataccaca taatctttta agttatttaa atttaactct
     4561 caggcttttc tgtcagtaaa atcagaaaaa ttgtagaatt tcgttttttg ctgcgaagtg
     4621 aaatgtatct catttttatt gccttcgccg gtgcgtcagt caatggcagg tagtgtacct
     4681 gcattctgct gtaacgcgta atgtcgggta gtttacttaa acttaatatg ttttatgttc
     4741 gcattattat aaatcacttt attgcctttt gtctgctttt taatggttcc cgttttgttc
     4801 acttctggct ttataaaaaa tggatgctga agaaatgaag gcagtccacc ttacataata
     4861 ttcatgcatg atttttatgc tattccgact gagtggaaga aaataaagga gaaaagaggg
     4921 tatatatgaa cctcagaaaa aaacatatct gtatagccat atcacttgtt cttaatccgg
     4981 catccatatc acaggccagt gctcaggcag atacaaataa gttaactgtt gaagttgatg
     5041 aaaccagcct tggccatgat gtctatggcg atgactggaa gagcattgaa ataggtacaa
     5101 cgaaatttac agcaaaagat gaatcaaagg gaattaattt cgaccataaa tcaccggtgg
     5161 ctgaaggacg taactctatc gcgattggta ccagtgcaga ggctggaggt gacattagca
     5221 cgaaggaagg tttgtacaca ggcgagtcta ttgcgatagg agcgaaaacg aaagcaacta
     5281 aagaacagtc aattgcaata ggtggcgata ccaaagctac cggatggggg gctattgtta
     5341 ttggtgggga tgatttaact cctttgcatg gtaagactta tggtcgggaa aaaatagaga
     5401 ataactatct ggcatcggaa gcgaaggggg atggcagtat tgcgctgggc agcagaactt
     5461 ttgctaaagg cgaattgtca gtatcgcttg gttcggtgag ttctgctgag gggatggcct
     5521 cgaatgcaat tggagccacg gcggaagcta aaggggatat ggcgactgcg gttggtttta
     5581 atgcgctggc taatgccggt aatgctctgg ccgttggtag tcagtcctct gcaaataaaa
     5641 acgatgctct tgctgttggt tcagaggcaa atgccgctca gaatgaaacg gttgccctgg
     5701 ggcatggagc aaccgttact gtcgcaaagg ggaacgtgtc catcggcgct gactccaggg
     5761 ataagcaggc acaaaaagta acagaggcta aggtcagaac taaaagaggc gcggaagtca
     5821 catatagtgg ctttgccgga aataacgcag catcagtagt atctgttggt gatgcgggta
     5881 aagagcgtca gattgttaac gtgggggcgg gggcgatttc tgacacaagt acagatgcca
     5941 ttaacggtag tcagttatat gcgatagcga agacgctgaa agatgacctt gatgccatta
     6001 atccgacgtg gacgctaaag gatgataagg gaggttctgc gactattaat ggtgggaagg
     6061 ataataagaa tggtaacact ttaactgtta aaggtgatgg agatattgca gttactgtta
     6121 atgataaagg aatgacgatt gacggaacta aacttactga aaacattaaa acagatgcta
     6181 taaaaataaa agataacaat ggtggtcttc tgaaaaaaga cctttcggat gaaatcgaaa
     6241 tcactggcaa aggcccaata aaaacccagg tggctggaga taaactggag atttcagcag
     6301 agacagcttc gttgcctgta aatggtggta acgggaagat tacagtaagc aatggtgatg
     6361 acgataagct tgttactgcg aaaaacattg ctgaagccat aaaccagtct ggctggaaaa
     6421 ttaaagcgga taaagatacc ggtggtgtta tcgagggaaa tgctcaggga acagaaacac
     6481 tgataaaacc cggtgatatg gttacgctga aagccggtaa aaatctcagc gtgaaacaaa
     6541 gtaacggtga ttttacattc tctgttattg atactccgtc attcagcagt gttcagctga
     6601 acggtaatgg aaaaaacgga aatgtaacgc tctcatccga aggtaataag ctgacattgt
     6661 caggcggtaa tggtgccgga caaaatggtg ttgttatcag tggtgtggca agtggattga
     6721 ataataagga cctggcaggc ctgaaagcag acgatgcgga aatgaaaaat gccgccagtg
     6781 ttgcagacct gaaaacggca attgatggtg tgtccaccag tatgcaggga gcaggtttcg
     6841 cactgaaaga ttctaaggga caggaagtga aacagtctct tgggcaggcg attaaagtat
     6901 ccggggacag caatatcacc accacagcaa tcactgccaa tggagataag gggttaaaaa
     6961 tatccctgaa taatgacctg actgtggggg ataaggctcc cggcagcatc aaagttaacg
     7021 gggctgatgg taaggaggct gtgagtctga atggtcagga tggcacgatt aaactgacat
     7081 cctcgcctgc tgcgggcgga aagtctgcgt cagcaaccat tgctgttaat gcaggcaaag
     7141 gtggattaac agatgctgcc ggtaccacga agacacgtct gacttatgct ccagatggaa
     7201 cattaccggc agaagagctg gcgaccatga atgacggtct ttcttttgag ggtgatgacg
     7261 gaaagattat taataaaaaa ctgaatgaaa aactgaccat caaaggtggt gcagagaaga
     7321 atgctgaagt gactgacggc aatatccggg ttgataatga taatggcggg cttctggtga
     7381 ggatgaccag aaacctccgt gatttggcca gcgcggtttt tggtacagaa aacggcccga
     7441 caacccggat tgacaatgca ggcatcacaa tcacaacacc tgctgcgggc ggtggtcagg
     7501 ggaatacggt ttcccttacc ggctccggcc tgaataacgg tggaaatcag ataaccagtg
     7561 tggccagtgg tctgaacggc actgcgcttg ctgacgccgg tggagatgtt ctgaataatg
     7621 cggccaatat cggtgacctg aaaaatgctc tccgtgacag cagtgcggca ctggtgaata
     7681 ccggctttgg tctgcaggcg gatgacggaa gcaaggtgaa cacgtctctc gggaagcata
     7741 tcacggttgg tggtgacggg cagaatatca gcacgactgt tcagaacggg agcctgacgg
     7801 tcagactcgc tgataacatt cgcctgggag atgacggcag cgtttctgtc ggcggaaccc
     7861 ggatggataa aaacgggctg accatcaagg atggcccgtc agtgactgtg aacggtattg
     7921 atgcggctgg ccgggttatt tccggcgtga aagacggggt gaatgaccag gacgccgtta
     7981 acgtttcaca actgaaccgt gaaatcagtc aggcaacggc agcaacgacc tgggcactga
     8041 agactgaatc cggtgatgag gctgttgctg tttcttccca gacagttgaa gtccgccacg
     8101 gacagaatac ccgggtttca gccatcagta aggatgacaa gggaaattac agctatgaga
     8161 ttgatgtgac ggggattccg gtggagtaca ccgacagcca ggggaacccg cttgtcaata
     8221 ttggtggtcg tttttacaca cagaccgaag atgctgcgac cgggaaactg acaatgacgc
     8281 cggctgagcc ggcccgtgtc agaataagca gtgagcagcc tcttgttctg acaaatgtgg
     8341 cggccggaaa tgtgtcaccg gtatccacgg atgcggttaa cgggagccag ctggcatctg
     8401 cagccagaat tgctggcggt gataatgttg tctggaaaga cggaaaagca gcgctggctc
     8461 ctgacacctt cagtgagctg acaactgcag gtggcggtaa gacaacggtg aatggtggtg
     8521 tacataaaac accggataat gtggctgatg cagtgaatat gctgaaccgt gaaggcacga
     8581 agtattttaa agctgcctct tccggtcagg cagcaaaagc ggaagggcgt gacagtattg
     8641 ctgtcggaca gggggccgtt tcccggggac aaaacagcat tgccatgggt aatggtgctg
     8701 aagtcacgcg tcaggctgct gccggctctg ttgcagttgg cagccatgcc cgtgctggcc
     8761 gcgcgaatac cgggacttat gccctgaacg gtcaggctgt tgcgggacgt accggaagtg
     8821 atactgcggt ggtttctttt ggtcagccgg gacaggagcg tcagctgcag tcagtggcac
     8881 cgggtgtgtt gtctgcgaac tcaacggatg ccgtcaatgg cagtcagttg catgcaacta
     8941 accgtcaggt tgccggaaat acacaggcga taaacactct cgggaataaa ttcagtcagc
     9001 tgagctaccg ggtggaagag cttaaccggg atatccgtgg tgttggcgca tcggctgcgg
     9061 ccatgagcgg gatacctcag gcctatctgc cgggtaaaag tctgatgggg ctgggtgtcg
     9121 gtggatatgg tggtgaaagc gccattgcaa taggtgtgtc ccgaatctcc gataacggta
     9181 agatgataat gaaactgaat gcaggtcaga acacccgggg taatttctcc gtcggtgccg
     9241 gtgtgggctg gcagtggtaa cagggaaaag caaggagaaa tgtaatgtta tttagtcgaa
     9301 tgatgatgac gggtgtgctg tccctgatgc ttgcaggatg tggaaatctg agtcatgtca
     9361 cggatgaagg aacgacggaa gaagtggtat ggccttctcc ggagaaggtg atgtttaatc
     9421 atgacggaac acagaaaggt tcgtggccac tgccagaaaa gttacgtcag ataaaggcgg
     9481 gtatgaataa ggaccagatt tatcagctgg cggggagacc acactttgct gaagggatgt
     9541 ctggtgtaag ggaatgggat tacctgttca actttaccgg accggagggg gattatacct
     9601 gccagtacaa gatactgttt gaccggaaca tggatgcccg ctctttctac tggaagccgg
     9661 aaaactgtct gccggaggca gagacagcag aatacaggga gtcagtcccc ggtgatatat
     9721 tatttgattt tgattcatgg actctgacag agagggggcg tgatgcactt tcccgtgtgg
     9781 cagagaagtt tcaggcgggt catgttgtcg gggtgaagat agaaggattt acggaccatc
     9841 ttggcgggca ggcgtataac cggctcctgt cagagaaacg ggcggaatca gtcagggggt
     9901 ggtttattgc ccggggattc agggctgaaa ggatacatgc aacgggccgt ggatatgatg
     9961 caccagtggt gttctgcccg gatgtggagg gcgaagcact gaaggaatgt ctgcgcccca
    10021 atcgcagggt tgaaataacc ggacagagtg ttcgttaata tgcagtgtag ccccggaatg
    10081 tccggggctt ttttattaat aggtgggttt tactttgctg catcctgttt gatatttctt
    10141 tgatgagaag tttgtcgcag ggtgattcat ctgaatgtta taatataacc tatctaggtg
    10201 tgcataagaa tacatagcta atgtattact gtttctaatg ttgttttttt gtaggaggga
    10261 ttctccttgc ggtagctttt tgtttttaga tgcttattgc ttaaagaata taatttttat
    10321 gcttttatat tgatttcttt acatttactg agttttctta tttatttaaa cattatgaaa
    10381 tctaatgctt ttgacgtcat ggggaaggtt gcatggctat gggcctgttc tcctctgcat
    10441 aaaaaatggc cattgtctgt ttttgctatt aatgtcatac ctgctattca gacaaatcaa
    10501 tttgctctct taataaagga tgagcttcct gtagcttttt gtagctgggc cagcttagat
    10561 ctggagtgtg aggtaaaata tataaatgat gtaacatctt tgtatgcaaa agactggttg
    10621 tcgggagaaa gaaagtggtt tattgactgg atagctccat ttggacacaa catggagtta
    10681 tataaacaca tgaggaaaaa gtatccttat gagttattca gagcaataag gttagatgag
    10741 tacagtaaaa cagggaaaat agctgaattt catggagggg ggatagataa aaaactggca
    10801 tcaaaaatat tcaggcaata ccatcatgaa cttatgtctg aagtaaaaaa cagacaagat
    10861 tttaatttta atattgagaa agaaaactaa tatgacagta aataaaataa agaacatttt
    10921 caacaatgcg acatcgacta caaaatcagc attcaataca gcatcatcaa gcgtacgttc
    10981 cgctggaaaa aaactcatat tattaatacc tgataattat gaagctcagg gcgtgggtat
    11041 taatgagttg gtcaaagctg ctgatgatct tggaatagaa atacaccgta ctgaacgaga
    11101 tgatacagcg attgcaaacc agttttttgg tacagcagaa aaagttgtag gattaactga
    11161 acgtggtgtt gcaatattcg caccacaact tgacaaactt ctgcagaagt atcagaaagt
    11221 tgggagtaaa ataggaggaa ccgctgaaaa tgtaggtaat aatctgggaa aagccggaac
    11281 agttctctca gcactacaga attttacggg gattgcttta tctggcatgg ctcttgatga
    11341 attgctgaga aaacaacggg aaggagagga tataagtcag aatgatattg ccaaaagtag
    11401 tattgaactt attaatcagc ttgtagatac agtatcaagt ataaacagta ccgttgattc
    11461 attttctgag cagcttaacc agcttggctc atttttatcc agtaaacctc gcttaagttc
    11521 tgttggtgga aaattacaaa atttaccgaa cctgggcccc ctgggggatg ggctggatgt
    11581 tgtctccgga attctttctg ctgtatcagc aagctttatt ctgggaaaca gtgacgcaca
    11641 tacaggaaca aaagctgcag cgggtatcga actgacaact caggttcttg gaaatgttgg
    11701 taaagctgtt tcgcaatata ttctggctca gagaatggca cagggattat cgacaacagc
    11761 tgcaagtgcg ggtctgatca catcggctgt tatgctggct atcagtcctc tttctttcct
    11821 ggctattgca gataaatttg agcgagctaa gcagcttgaa tcatattctg aacgatttaa
    11881 aaaattgaat tatgaagggg atgctttact cgcagccttt cataaagaaa ccggagctat
    11941 agatgcagcc ctgacaacaa taaatactgt cctgagttct gtatctgcgg gagttagtgc
    12001 cgcctccagt gcatccctca taggggcccc gataagcatg ctggtgagtg cattaaccgg
    12061 tacgatatct ggcattctgg aagcatcaaa acaggctatg tttgagcacg ttgcagataa
    12121 attcgctgcc cggatcaatg aatgggaaaa ggagcatggc aaaaattatt ttgagaatgg
    12181 ctatgacgca agacatgctg cgtttttaga agattctctg tctttgcttg ctgatttttc
    12241 tcgtcagcat gcagtagaaa gagctgtcgc aatcacccag caacattggg atgagaagat
    12301 cggtgaactt gcaggtataa cccgtaatgc tgatcgcagt cagagtggta aggcatatat
    12361 taattatctg gagaatggag ggcttttaga ggctcaaccg aaggagttta cacaacaagt
    12421 ttttgatcct caaaaaggga ctatagacct ttcaacaggt aatgtatcaa gtgttttgac
    12481 atttgtaaca ccaacattta ccccaggaga agaagtcaga gaaagaaaac agagtggtaa
    12541 atatgaatat atgacatctc ttattgtaaa tggtaaggat acatggtctg taaaaggcat
    12601 aaaaaatcat aaaggtgtat atgattattc aaatttgatt cagtttgttg aaaaggataa
    12661 caaacactat caggcgagaa taatttctga gctcggagat aaagacgata tagtttattc
    12721 tggggcaggc tcatcagaag tatttgctgg tgaaggtcat gataccgtat cttataataa
    12781 gacggatgtt ggtaaactaa caattgatgc aacaggagca tcaaaacctg gtgaatatat
    12841 agtttcaaaa aatatgtatg gtgacgtgaa ggtattgcag gaagtcgtta aggaacagga
    12901 ggtgtcagta gggaagcgaa cagagaaaat acaatatcgt gattttgaat tcagaaccgg
    12961 tggaattcct tatgatgtga tagataatct tcattctgtt gaagaactca ttggcggaaa
    13021 acatgatgat gaattcaaag gcggtaagtt taatgatata ttccatggcg cagatgggaa
    13081 cgattatatc gaaggtaatt atggtaatga tcgactatac ggcgatgata gggatgatta
    13141 tatatccgga ggacagggag acgaccagtt atttggtggt agtggaaacg ataaattgag
    13201 tggaggggat ggtaataatt atctgacagg aggaagcggt aatgatgagc ttcaggcaca
    13261 cggagcttat aatattctgt caggtggtac tggtgatgat aaactttatg gtggtggtgg
    13321 tattgacctt ctggatggag gggaaggtaa tgactatctg aatggtggtt ttggtaatga
    13381 tatttatgtt tataggcaaa actatggtca tcatacaatt gcagatgaag gaggtaaagg
    13441 agatcgtctg cacttatctg atattagctt tgatgatatc gcatttaaga gagttggaaa
    13501 tgatcttatc atgaataaag ccattaatgg tgtactttca tttaatgagt caaatgatgt
    13561 caatgggata acatttaaaa actggtttgc gaaagatgcc tcaggagcag ataatcatct
    13621 tgttgaggtt ataacagata aagatggtcg tgagataaaa gctgataaga tacctcataa
    13681 taataatgaa cggtcaggtt atataaaagc cagtaatata gcatctgaaa aaaacatggt
    13741 taatatcacc agtgttgccg atgatattaa caagattatt tcttcagttt cagggttcga
    13801 ttcaggtgat gaacgattag catctttata taatttatcc ttacatcaaa acaacacaca
    13861 ttcaacaact ttaacgacaa ctgtctgaga ctaaaagcgg aggtgatggc cattccagga
    13921 ggaatggtca tcgttatata atgagtaaat gtagttctca taatagtctg tatgcactga
    13981 tattgcttgc acaatatcat aatataactg tcaatgctga aactataagg catcagtata
    14041 atacccacac acaagatttt ggggtgactg aatggttact ggcagcgaaa tccattggct
    14101 taaaagcaaa atatgtagaa aaacattttt ccagattgtc aataatttct ttacctgcgt
    14161 tgatatggcg ggatgacggt aagcattata tattatctcg tgttactaaa gattcatcac
    14221 gctatcttgt ttatgatcca gaacagcatc agtcactaac ttttagtcgg gatgagttcg
    14281 aaaaactgta tcagggaaaa gtcattctgg ttacgtcaag agcaacagta gtcggagagt
    14341 tagctaaatt tgatttttcc tggtttatcc cctctgttgt gaaatacagg aggattttac
    14401 ttgaggtgtt aactgtttct gcttttattc agtttcttgc gttaataaca cctctttttt
    14461 ttcaggttgt aatggataag gttttagttc accgggggtt ttcaacgtta aatattatca
    14521 caatagcatt tattatagtg atactttttg aagtgatatt aaccggagcc agaacttata
    14581 ttttctctca tactacaagt cgtattgacg tcgaactggg tgctaagtta ttcagacatt
    14641 tgcttgcatt gcctgtttca tattttgaaa atcgcagggt cggagagacc gttgccagag
    14701 taagggaact ggagcaaatt cgtaattttt taaccggaca agcgttgaca tcagttcttg
    14761 atctgttttt ttctgtaata tttttttgtg tcatgtggta ttacagtcct caattaacac
    14821 tggttatatt attgtcacta ccttgttatg ttatatggtc attgtttata tcacccttat
    14881 tacgtcgacg tcttgatgat aagtttctca ggaatgcaga aaatcaagct tttcttgtcg
    14941 aaacggtaac agcaataaat acaattaaat ccatggcagt atcgccacag atgattgcta
    15001 catgggataa acaactggcc gggtatgttg cttccagttt cagggtaaat ctggttgcaa
    15061 tgacagggca gcaggggata cagctgatac agaaaagtgt aatggtaatt agtctatgga
    15121 tgggagcaca tcttgtcata tcgggagaga taagcatcgg gcagttaatc gcgtttaata
    15181 tgcttgcagg tcaggtcatt gctcctgtta tcagactggc tcatctttgg caggattttc
    15241 agcaagttgg tatttctgtt gagcgccttg gggatgtatt aaatacaccg gtagagaaaa
    15301 agtcaggcag aaatatactg ccggaaattc agggggatat cgaatttaaa aatgtcagat
    15361 ttcggtattc ttctgacggt aatgttattt tgaataatat taatttatac atatcaaagg
    15421 gggatgttat cggtatagtt ggccgttctg gttcaggaaa aagtacatta acaaaactgc
    15481 ttcagcgctt ttatatacca gagaccggac agattttaat tgatgggcat gatttatcac
    15541 ttgcagatcc agaatggtta cgacgccaga ttggtgttgt attacaggaa aatatactat
    15601 taaatcgtag tattatcgat aatattacat tagcttctcc ggctgtatct atggaacagg
    15661 ctattgaggc agccagactt gcaggtgccc atgattttat tacagaacta aaagaagggt
    15721 acaatactat tgttggagaa cagggcgttg gtctttcagg agggcagcgc caacggatcg
    15781 caatagcccg ggctcttgtt acaaatcctc gaattcttat ttttgatgaa gcaaccagtg
    15841 ctcttgatta tgagtcggaa aatataataa tgaaaaatat gtcaagaata tgtaagaaca
    15901 gaaccgtaat tattattgca cacaggttgt caactgtaaa aaatgcaaac agaataattg
    15961 ttatggataa tggatttatt tctgaagatg gcacacataa agagcttatc tccaaaaaag
    16021 acagtttata tgcatattta tatcagttgc aggcataacg tatgcgcttt tacatgaaag
    16081 gtttatggga tttgatttgc cgttataaga cggttttttc tgatgtctgg aaaattcgtc
    16141 acacacttga tgctcctgtg agagaaaaag atgagtatgc atttcttcct gctcaccttg
    16201 aactcattga aacaccagta tccagacgtt ctcattttgt tatatggagt attttattat
    16261 ttgtaattat atctcttctc ttatctgttc ttgggaaagt tgaagtggtc tcagtagcaa
    16321 atggtaagtt tactcatagt ggaagaagca aagaaataaa accgatagag aatgcgattg
    16381 tagaaaaaat aatggtgaaa gatggcgctt ttgtaaaaaa aaatgatcca ttagttgaat
    16441 tgacggtgcc tggtgttgaa tctgatattt taaaatcaga agcatctttg ttgtatgaaa
    16501 aaacagaaca atatcgctac gcaatccttt ctgaatcaat acaacggaat gagcttcctg
    16561 aaataaggat aacagatttt cctggcggag aagataatgc aggaggtgaa cattttcaga
    16621 gggttagctc attaataaaa gagcaattca tgacatggca gaacagaaaa aatcaaaagc
    16681 agctcacatt aaataaaaaa atagttgaac gggatgcagc gcttgctcgt gttagcctgt
    16741 atgagcatca ggtatcacag gaaggaagaa aacttaatga ttttaagtat ttgttgaata
    16801 aaaaagctgt ttctcaacat tcagttatgg agcaggaaaa tagctatatt caggcaaaaa
    16861 atgaacatgc agtctggctt gcacaggttt ctcaacttga aaaagaaata gaacttgtgc
    16921 gggaagaact ggcactggag acgaatatct tcagaagtga aattatcgag aagcacagaa
    16981 aatcaacaga taacattgtg ttactggagc atgagcttga aaaaaacagg cagagaaaag
    17041 catcatcttt tattaaagct cctgtgagtg gtactgttca ggagttaaat atacatacag
    17101 aaggtggtgt tgtcacaaca gcagaaacgc tgatgattat tgtccctgat aatgatattc
    17161 tcgaagtaac agcatctgta cttaacaagg atatcggttt tatacaacct ggacaggagg
    17221 tcgttattaa agtagatgca tatccatata cacgtcatgg ttaccttaca gggaaagtaa
    17281 aaaatataac tgcagattct gtttctgttc cggatacagg acttgtattt aacgtgatta
    17341 tatctgttga tcggaatgat atacagggag aaagaaaaaa aattcctgtt acagctggaa
    17401 tgactgttat ggcagaaata aaaacaggag tgcgtagtgt tatcagctat cttcttagcc
    17461 cattaaagga aactattaat gaaagtttcc gtgaacgtta gtatataaat aacatattgg
    17521 ctttttatat a
//
LOCUS       DAZGAN020000103        17212 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGAN020000103 DAZGAN020000000
VERSION     DAZGAN020000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.103"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(61..1101)
                     /locus_tag="ACHKYO_RS18750"
                     /old_locus_tag="pgaptmp_003750"
     CDS             complement(61..1101)
                     /locus_tag="ACHKYO_RS18750"
                     /old_locus_tag="pgaptmp_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGU4430520.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(1174..1809)
                     /locus_tag="ACHKYO_RS18755"
                     /old_locus_tag="pgaptmp_003751"
     CDS             complement(1174..1809)
                     /locus_tag="ACHKYO_RS18755"
                     /old_locus_tag="pgaptmp_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU4430521.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            1937..2455
                     /gene="yhbO"
                     /locus_tag="ACHKYO_RS18760"
                     /old_locus_tag="pgaptmp_003752"
     CDS             1937..2455
                     /gene="yhbO"
                     /locus_tag="ACHKYO_RS18760"
                     /old_locus_tag="pgaptmp_003752"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HGU4430522.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(2435..2878)
                     /locus_tag="ACHKYO_RS18765"
                     /old_locus_tag="pgaptmp_003753"
     CDS             complement(2435..2878)
                     /locus_tag="ACHKYO_RS18765"
                     /old_locus_tag="pgaptmp_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HGU4430523.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            2929..3231
                     /gene="yhbQ"
                     /locus_tag="ACHKYO_RS18770"
                     /old_locus_tag="pgaptmp_003754"
     CDS             2929..3231
                     /gene="yhbQ"
                     /locus_tag="ACHKYO_RS18770"
                     /old_locus_tag="pgaptmp_003754"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HGU4430524.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAESAGFAELLSSLQTPEIKSD"
     gene            complement(3218..3721)
                     /locus_tag="ACHKYO_RS18775"
                     /old_locus_tag="pgaptmp_003755"
     CDS             complement(3218..3721)
                     /locus_tag="ACHKYO_RS18775"
                     /old_locus_tag="pgaptmp_003755"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4430525.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(3715..4239)
                     /locus_tag="ACHKYO_RS18780"
                     /old_locus_tag="pgaptmp_003756"
     CDS             complement(3715..4239)
                     /locus_tag="ACHKYO_RS18780"
                     /old_locus_tag="pgaptmp_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HGU4430526.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            4448..5443
                     /locus_tag="ACHKYO_RS18785"
                     /old_locus_tag="pgaptmp_003757"
     CDS             4448..5443
                     /locus_tag="ACHKYO_RS18785"
                     /old_locus_tag="pgaptmp_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGU4430527.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            5452..6330
                     /locus_tag="ACHKYO_RS18790"
                     /old_locus_tag="pgaptmp_003758"
     CDS             5452..6330
                     /locus_tag="ACHKYO_RS18790"
                     /old_locus_tag="pgaptmp_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGU4430528.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGISN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            6536..7543
                     /locus_tag="ACHKYO_RS18795"
                     /old_locus_tag="pgaptmp_003759"
     CDS             6536..7543
                     /locus_tag="ACHKYO_RS18795"
                     /old_locus_tag="pgaptmp_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HGU4430529.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(7642..8217)
                     /gene="dolP"
                     /locus_tag="ACHKYO_RS18800"
                     /old_locus_tag="pgaptmp_003760"
     CDS             complement(7642..8217)
                     /gene="dolP"
                     /locus_tag="ACHKYO_RS18800"
                     /old_locus_tag="pgaptmp_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HGU4430530.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(8227..8817)
                     /gene="diaA"
                     /locus_tag="ACHKYO_RS18805"
                     /old_locus_tag="pgaptmp_003761"
     CDS             complement(8227..8817)
                     /gene="diaA"
                     /locus_tag="ACHKYO_RS18805"
                     /old_locus_tag="pgaptmp_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HGU4430531.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            complement(8837..9232)
                     /locus_tag="ACHKYO_RS18810"
                     /old_locus_tag="pgaptmp_003762"
     CDS             complement(8837..9232)
                     /locus_tag="ACHKYO_RS18810"
                     /old_locus_tag="pgaptmp_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HGU4430532.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            complement(9190..11226)
                     /gene="lpoA"
                     /locus_tag="ACHKYO_RS18815"
                     /old_locus_tag="pgaptmp_003763"
     CDS             complement(9190..11226)
                     /gene="lpoA"
                     /locus_tag="ACHKYO_RS18815"
                     /old_locus_tag="pgaptmp_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HGU4430533.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            11290..12150
                     /gene="rsmI"
                     /locus_tag="ACHKYO_RS18820"
                     /old_locus_tag="pgaptmp_003764"
     CDS             11290..12150
                     /gene="rsmI"
                     /locus_tag="ACHKYO_RS18820"
                     /old_locus_tag="pgaptmp_003764"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGU4430534.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQKGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            complement(12193..13284)
                     /locus_tag="ACHKYO_RS18825"
                     /old_locus_tag="pgaptmp_003765"
     CDS             complement(12193..13284)
                     /locus_tag="ACHKYO_RS18825"
                     /old_locus_tag="pgaptmp_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4430535.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(13295..15811)
                     /locus_tag="ACHKYO_RS18830"
                     /old_locus_tag="pgaptmp_003766"
     CDS             complement(13295..15811)
                     /locus_tag="ACHKYO_RS18830"
                     /old_locus_tag="pgaptmp_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4430536.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFNEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            complement(15841..16536)
                     /locus_tag="ACHKYO_RS18835"
                     /old_locus_tag="pgaptmp_003767"
     CDS             complement(15841..16536)
                     /locus_tag="ACHKYO_RS18835"
                     /old_locus_tag="pgaptmp_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4430537.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            complement(16616..17200)
                     /locus_tag="ACHKYO_RS18840"
                     /old_locus_tag="pgaptmp_003768"
     CDS             complement(16616..17200)
                     /locus_tag="ACHKYO_RS18840"
                     /old_locus_tag="pgaptmp_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4430538.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
BASE COUNT         4081 a         4561 c         4352 g         4218 t
ORIGIN      
        1 cgatgcaaac cgtaggtcgg ataaggcgtt tacgccgcat ccgacatgtt ttccctcaaa
       61 tcagaacaac agcgccagcc cgccgacaat cacaccagac actgccacca tcgcccctgt
      121 cagccataag gctttcgctg ggaacgcttt gcgcagcata atcagtgacg gcaaactcac
      181 cgccgggagc gtcatcaaca atgccagcgc cggagcagtt cccatacctg ccagcatcat
      241 cgtttgtaca atcggaattt ctgctgccgt gggaatgaca aacaagcatc ctgctaccgc
      301 catcgccatc acccacatca ggctgttatc gacagcacca tcggcatggg ggaataacca
      361 gacgcgagcg gcacccaaca ccagtactgc aaggatgtaa accgggatcg tactccagaa
      421 aagcgtccat agcgccctgc cccagcggct aaaaaaaccg ccctgtgctt ccggtatgtc
      481 aatttcgacc ggtgcctgcg tttgcggtgt ttcacgcacc catttttgca ccagcgtcgc
      541 aatcagcaac accatcacca gcccggccac cagacgaatc gccgcaaaac cccagccgag
      601 gacaaacccc ataaacacca gcgtcgccgg gtttaacacc ggattgccca tccagaatgc
      661 cagcgcaccg cccatcgaca cctgttggcg acgcattccc gccgcgaccg gagccgcaca
      721 gcaggtacac atcatgcccg gcagcgaaaa cagcgttccc agcagcgtgc cgcgaaagcg
      781 cgattgccca agcgtacgca acaaccagtc acgcgggatc agcacctgaa tcaacgaacc
      841 gaggatcacc cccagcactg ccgctttcca taccgcgagg aaatagatca tcgcgtaatc
      901 caacgccgcc tgccacggat tagcatccgc ctgcgcaagg atagatttac cgatactgtg
      961 ggtttcggca gcagtaaacg ctttgccgta gtaaggttgc catttcacat accagagacc
     1021 ggcaatgaca acgagaaaga aaagcgcggg tttccaccac tgaatgggcg ttgccgcctg
     1081 agatgaagac tgaccagtca tagcattccc caggaataag ttatgataat tgagcgcgtg
     1141 aatattacgc tcactatcaa ttcttgggaa taattattca gctcttttgc gtaattctga
     1201 agagcttaaa atcgtcacac cttcatgccc gggccgcatc gcctctgcca gcatgacccg
     1261 cgccacgtct ctcgcatcaa tggatttcca gttaccgggt aataaacgga acagcggcgc
     1321 aaaaagcgtt tcgttcatcc gctgtttgct acgatcgccc agtaacatcg acgggcgagc
     1381 aatggtcaat ttcggccagt tctgggcgat taatgcttct tccatctccc ctttgacgcg
     1441 gttatagaaa aacggcgagt gggcattggc acccatagca ctgaccacca gcatatgctg
     1501 cgcgcccagt cgccgcccgg ttaatgcggt atccactacc agcgtgtaat cggcatgaat
     1561 aaacgcctct ttgctccccg cttctcgccg cgtggtgccg agacaacaaa acacaatgtc
     1621 gatgggatcg gtgacctgcg ccagcgcgtc agtcagttgc ggatcatggg gattaaacac
     1681 cccgggcata tcgcccaacg gacgtcgcgt cggcgcggct atggcgttaa ctttcggttc
     1741 gttaatcaac atccgcagca ggtgaccgcc caccagcccc gttgcgcctg taatcagtac
     1801 ctgactcatc ttcgctcctt tacagaattg tctgccttgc gctccacggc tcatgcacca
     1861 ggcttaatag accatgaggt aattatcccc gattgtggga aattcgcctc atccaatgca
     1921 acaacgcgga ggaagcatga gtaagaaaat tgccgtttta atcactgatg aatttgaaga
     1981 ttcagaattt acttcacccg cagacgagtt ccgtaaagcc ggacacgaag tgattaccat
     2041 tgaaaaacaa gcgggtaaaa cggtgaaagg caaaaaagga gaagccagcg tgaccatcga
     2101 taaatccatc gatgaagtga cgccagcgga gtttgatgcc ctgctgctac cgggcggcca
     2161 ttcaccggat tatctgcgtg gcgacaaccg ttttgtcacc tttacccgtg attttgtgaa
     2221 tagcggcaaa cctgttttcg ccatctgtca cggtccgcag ctgctgatca gcgccgatgt
     2281 gattcggggg cgcaaactga ccgcggttaa accgatcatt attgatgtta aaaacgcggg
     2341 cgcggaattt tacgatcagg aagtcgtggt cgataaagat cagctggtta ccagccggac
     2401 accggacgat ctgccagcgt ttaaccgcga ggcgttacgc ctgctcggtg cctgagccgc
     2461 gtagccaaat cattttttta ccaaagccca gcgtgttgtc ggtgaatttg atttcatcga
     2521 gccggatttc ccacaccggg gccgacagca ttctggcaac cggaaagcga cgattgtacg
     2581 ccttgcgcgc gaggtcgctt tcctcacctt ccagcctgcg gatctcacct ttaaactgca
     2641 cgccgcgaat taacgctacc gttttcggct gaccgtttac cgttccggca accgcagcct
     2701 gcggcccact catctgcgcg tggcgcgttt tttcttccgt caaaatgtag aaggcgactt
     2761 tctgcgcatc aaacagataa aaagcattag cgcaccagag ttccccttcc tgctgcacac
     2821 accaggtgac aacatgttgt tttgccaacc aacggctgat ggtgatgagt gtttccattg
     2881 ctgttctctt ttatactgtg ggcctgacct taacatgcga cgcagacgat gacaccctgg
     2941 tttctttact tgatccgtac cgccgacaat aagctttata ccgggatcac cacggatgtc
     3001 gaacgccgct atcagcagca ccaaagcggc aaaggggcga aagcactgcg cgggaaagga
     3061 gaactgacgc tggcgttttc tgcgccagtg ggcgatcgtt cgctggcgtt acgggcggaa
     3121 tatcgcgtta agcaactgac caaacggcaa aaagagcgtc tggtagcgga aagcgcaggg
     3181 tttgcagagc tgttaagcag tctgcaaacc ccggagatta aaagcgattg aaatgctcgt
     3241 gatactcaac cagaccagtt acgccattca acgcgtcatc cgctaaacga tgtacctgga
     3301 aggcgctttc agtgcctggc cagcggcaac gcaggtcgtg atgtgccgcc agttcaaagc
     3361 caaaacggct gtacagcgcc ggatcgccca gcgtcaccac tgcggcatag ccgaactcat
     3421 taagcgaatc gagtccttca tagaccagtt ggcgtgccag cccctgtcca cggtattttt
     3481 catcaaccgc caacggtgcc atgccgaccc attgcaggtc ttcgccctgc acatcaaccg
     3541 gactaaatgc cacatagcca atgacctgac cttcgtcatc tgtcgccacc agccccagcg
     3601 tcagaaagcc atcttcacgc agatcgtgaa ccagcttcgc ttccgcatca ctttcgaatg
     3661 agcgacgtag cagggcatca atacccggcg catcaatggg aatttctact cgaattagca
     3721 tggttcacct accgatgtct gtttggtttc aggcgcggtt ttcattcccg cctcaacaaa
     3781 atccgccagt tgcagcagca tcatgcgtaa ggctttcggc atttgctcca gttcaatggc
     3841 gtccatcagg tttttcacat acagccccag ctccgtatcg ccttcaatca ccagccgacg
     3901 ctggaagaag agcgtatccg gatcttgttt acgcgccgca atcatcagca gatcgctggc
     3961 gtcggcacta aaactcacat cagcttgcgc gttctggctg accaccagtt tgccattcac
     4021 caccgaggta aaccattgca ggtcaatatc acgcacatga atacttaacc agcggccttc
     4081 aagaaactcc agctcgccat catccagcgc ctgacggaat tgccagctta agacctgctc
     4141 aagaacctgg cgttttagcg caaatggcgt cagttttacc ggtacactca acagagatgg
     4201 ccccaaatgc acaatacggg aacgcagttt atccaacacg agttttactc cctgtttcaa
     4261 caatcatcct attttgccat atcagaaaaa taacatagcg gtataaatca acaattccat
     4321 atgaaattgc tgctaccacc aatacaactt taactgcctt aaatcaaaaa ttgtcgcagc
     4381 aaggttaact aaaatcccag ttcgttaaca tttttgcgtt ttgatagcgc aaccttcagg
     4441 aaaaattatg gagctgctct gccctgccgg aaatctcccg gcgcttaagg cggccatcga
     4501 aaacggcgca gatgctgttt atatcgggct aaaagatgat accaatgccc gtcacttcgc
     4561 cggccttaac tttaccgaga aaaaattgca ggaagcggtg agttttgtcc atcaacatcg
     4621 ccgcaaactt cacatcgcga ttaacacttt tgcgcatccg gacggttacg cccgttggca
     4681 gcgtgccgtg gatatggcgg cgcagctggg tgccgacgcg ctgatcctcg ccgacctcgc
     4741 catgctagag tacgccgccg agcgttaccc gcatattgag cgtcatgtgt cagtgcaggc
     4801 ttcggcgacc aatgaagagg cgattaactt ttatcatcgc cattttgacg ttgctcgcgt
     4861 ggtgctgccg cgcgtgttgt cgattcatca ggtgaaacaa ctggcacggg tcacacctgt
     4921 accactggaa gtctttgctt tcggcagcct gtgcattatg tcggaaggtc gttgctatct
     4981 gtcgtcgtat ctgacgggtg agtcgcccaa caccgtaggc gcgtgttctc cggcccgttt
     5041 cgtgcgctgg caacaaacgc cgcaggggct ggaatcccgc ctgaacgaag tgctgatcga
     5101 ccgttatcag gacggcgaaa acgcaggtta tccgacgcta tgtaaagggc gttatctggt
     5161 ggacggcgag cgctatcacg cgctggaaga accaaccagt ctcaataccc tggaactgct
     5221 gccggagtta atggcggcga atattgcttc ggtgaaaatt gaaggccgcc agcgtagccc
     5281 ggcgtatgtc agccaggtgg cgaaagtctg gcgtcaggct atcgaccgtt gtaaggccga
     5341 tccgcaaaac ttcataccgc aaagcgcgtg gatggagacg ctcgggtcga tgtccgaagg
     5401 cacgcagacc acccttggcg cgtatcaccg taaatggcag tgagaagagc aatgaaatat
     5461 tccttagggc cagtgctgtg gtactggcca aaagagacgc tggaagattt ttatcagcag
     5521 gccgccacca gcagcgccga cgtgatttat cttggtgaag cggtatgcag caagcgccgg
     5581 gcaaccaaag ttggcgactg gctggagatg gcaaaatcgc tcgccggaag tggcaagcag
     5641 attgtgctct ccacgctggc gctggtgcag gcatcctctg aactgggcga actgaaacgc
     5701 tatgttgaga acggtgagtt tctgattgaa gccagcgatc tcggcgtggt gaatatgtgc
     5761 gctgaacgca aactgccgtt cgtcgccggg cacgcgctga actgctacaa cgcggtgacg
     5821 ctgaaaatat tgctcaaaca gggcatgatg cgctggtgta tgccggtgga gctttcccgc
     5881 gactggctgg tgaatctgct taatcagtgc gatgagctgg gcattagcaa ccagtttgaa
     5941 gtggaagtcc tgagctacgg tcatctaccg ctggcctact ccgcccgctg ctttaccgcg
     6001 cgttcggaag accgcccgaa agatgagtgt gaaacctgct gcattaagta tccgaacggg
     6061 cgcaacgtgc tgtcgcagga aaaccaacaa gtgtttgtac tcaatggcat tcagaccatg
     6121 agcggctacg tttacaacct cggtaacgag ctggcatcca tgcaggggct ggttgatgtg
     6181 gtccgcctgt caccgcaggg tactgacact ttcgcgatgc tcgacgcctt ccgcgctaat
     6241 gaaaatggcg ctgcgccact accgttgacg gcaaacagcg actgtaacgg ctactggcgg
     6301 cggctggccg ggctggagct gcaagcctaa gtaaatatgc tcgtcatact tcaagttgca
     6361 tgtgctgcat ctgcattcgt tcaccccagt cacttactta tgtaagctcc tggggattca
     6421 ctctcttgtc gccttcctgc aactcgaatt atttagagca cagctcactt tgttaacaac
     6481 tttaactact ctttaatgca gtattaaaga ttaatcggta acaaagtgag ctgttatgac
     6541 tgataaaacc attgcgtttt cgctactcga tctggccccc attcccgaag gttcttcagc
     6601 gcgagaagca ttctcccact ctctcgatct cgcccgtctg gctgaaaagc gcggctatca
     6661 tcgctactgg ctggcagaac accacaatat gactggcatt gccagtgctg ccacgtcggt
     6721 gttgattggc tatctggcgg cgaataccac cacgctgcat ctggggtctg gcggcgtgat
     6781 gttgcctaac cactcaccgt tggtcattgc cgaacagttc ggcacgctca atacactcta
     6841 tccggggcga atcgatttgg ggctgggtcg tgcgccaggt agtgaccaac ggacaatgat
     6901 ggcgctgcgt cgccatatga gcggcgatat tgataatttc ccccgcgatg tcgcggagct
     6961 ggtggactgg tttgacgccc gcgatcccaa tccgcatgtg cgcccggtac caggctatgg
     7021 cgagaaaatc cccgtgtggt tgttaggctc cagcctttac agcgcgcaac tggcggcgca
     7081 gcttggtctg ccgttcgcgt ttgcctcaca cttcgcgccg gatatgttgt tccaggcgct
     7141 gcatctttat cgcagcaact tcaaaccgtc ggcacgactg gaaaaaccat acgcgatggt
     7201 gtgcatcaat attatcgccg ccgatagcaa ccgcgacgct gagtttctgt ttacctcaat
     7261 gcagcaagcc tttgtgaagt tgcgccgtgg cgaaaccggg caactgccgc caccgattca
     7321 aaatatggat cagttctggt cgccgtccga acagtatggt gtgcagcagg cgctgagtat
     7381 gtcgttggta ggtgataaag cgaaagtgcg tcatggcttg cagtcgatcc tgcgcgaaac
     7441 cgacgccgat gagattatgg tcaacgggca gattttcgac caccaggcgc ggctgcattc
     7501 gtttgagctg gcgatggatg ttaaggaaga gttgttggga tagtgtgtct taacgcggga
     7561 agccgtatcc gagctggcaa cgctgtccta caaggcctga taagacgcgg caagcgtcgc
     7621 atcaggcatt acaaggggct gctatttaat aaacgtaaac gctgtggtta cccgctttac
     7681 gccgctcacc cggctggcaa tatctgccgc cgctttcgct tcacgttcag tcaccagccc
     7741 catcaggaac acttcaccgt tttcggtggt cactttcacg ttggacgatt tcaccaggtc
     7801 gctggttaag agctgcgaac gcactttggt ggtgatccac gtatcgttag acgcttcgcc
     7861 cagaccaatc ggctggccct gacgaatctc gttatacact tcgttggcac cgtctacgcc
     7921 catagcaatc tgtttggcgc gagccgaaag ttcagcgttt ggtgactgcc caaccagcag
     7981 cactttgccc tgatacgccg tgacattaat acgggcttct ttcttaatct gttcgtcttt
     8041 cgacaatgcg ctgttcacgc gcacttccag ggtaccatcg tccacctggg tgccgacact
     8101 gcgtgggtca gttgcggctt tggtacccac agcagcggta cccactacgg cagcggcaac
     8161 acaaccttgc aacagcagcg cggaaataag gactgcgatt ggcgataatg ccttcatgta
     8221 ttctccttaa acatcctggt gagggaaaag cgtgttatcg atcagatcgc acaggcaatt
     8281 taccgtcagc atatgcattt cctgaatgcg agcactacga tgcgaaggaa tgcggatctc
     8341 cacatcttgt ggccctaaca aacctgcaag ttcgccgccg tcatagccgg tcaatgccac
     8401 aatagtcata tcacgcgtaa cggcagcttc taccgctttt acaatatcgc ggctgttgcc
     8461 acgggtggaa atggctaaca atacatctcc cgcatgaccc agcgcccgca cctgttttgc
     8521 atacacttca tcatgtaagc gatcgttggc aatcgccgtt aagacaacat tatcagtatt
     8581 tagtgcaatg gcaggtaagc tgggccgctc cgtttcgaaa cggttgatca tgctggcagc
     8641 aaaatgctgt gcattggcag cggaagttcc attaccacaa cagaggattt tgttgccatt
     8701 gagcagagac tgaaccagcg tcatggctgc acgggagatg gcatccggaa gcgcctctgc
     8761 cgccgcaatt tgagtttgaa tgctttcagt gaagcaagct ttaattcttt cttgcacgct
     8821 aatccttaaa ccttaattat gagtggtcat taaaggcatc cttaatccac tcaacctcat
     8881 tcccggtgaa ggctaccaca tcgaaccggc aatccacagt atcaaaactc ccattatgac
     8941 gcgcgagcca caagcgggca gtctgtaata atttgtgttg tttgctgcgg gtcacactgg
     9001 ctgccgcgcc gccataaagc gcagagcggc ggtaacgcac ctcgacaaaa acggtggtcc
     9061 ggccttcacg cattatcaga tcgatctcgc cgccacgctc gttcacgtta gcggcgacaa
     9121 atcgcagtcc tttgccttcc agccagcgac gcgcttgtac ttcccacgca tcgccggtct
     9181 gtttggtggt taactggcgg ggactacctg accttgttgg tactgtagcc atgataactt
     9241 cctgttaatc acgcaatccg ggtttgccgt caggcttccg gtattaccgt tgatttcaaa
     9301 accctgaacc tggcgcattt gtgagaaatg atttgccagc gaccaggcat cgacgcccat
     9361 cgcatacatg cgagccagtg aataatcgtt attcaccgcg ctgagtgcct gctgcattaa
     9421 cggtagatta ccgcctgcca gcatcgggat ttcgctgtac tgcaagcctt ccatctccag
     9481 tcggaaatcc gggccagcgg tcccttgcgc actgcgggag ctggcgtaca gcgttgcacc
     9541 gctctggcta ccgttacgca tggcgatcat cggtttgata aaagcgattt cacccggcgt
     9601 tgccacaatg tacaccgcat cgacacgacc gccattattg gtgaactggt catcggttgg
     9661 cgtggtttgc agcgttggat tgttggtcgt catgccggag tcggttgtcg ctctgggagt
     9721 aatcggggta cccgttaaag cgataccaga accgccgtta acacccgcgc gtaattcgct
     9781 ggtggaacca aatttttgtt gcagaacggt gccgccgccc agtttctgcc actcctgcgc
     9841 aaacgcattg gctacgcgat cgcccaatga actgcgtggg atcagcacca gcggcgcttg
     9901 tttaccctgg tcacgaatat gacgcgctgc atcgcgcgct tcgtcttccg gtgaaagcgc
     9961 gaagtaacaa atattgacgc ggttttcgat attctccggc tggttcagtg ccagcacgtt
    10021 cagcggggtg ttgctcttaa gcaactcttc aacgttattt ttcagcaacg gaccaaccac
    10081 aatactcgcg ccatcctgct gaacctggct taagatctgg ctaagtggtt gtgatgaggt
    10141 atcgtagatt ttcagttctg cggatggatt cgctggcgcg cttaccgctg tggtggttgc
    10201 tgccggggtg cttacaggcg ctggctgtgc cgcaggctgt tcaccggtca gatcgctaac
    10261 cgaggcttgt gccgggctgg caacgccatc cacggtttgc ggctgaggtt gttcagctac
    10321 gtctgcggca ggtgcggcag ctacctgagc cgccactggc tgagtgccga tatttttcgc
    10381 cgcttcaaag ccttgctgaa tagtgcgacc aaataccgct gcctggccat tcagcggcaa
    10441 cagcagggcg attttgttgg tcgaggctgg tttaaacgct tttacgttaa ccaactgcgt
    10501 tggcagcatt ttcgcgcccg gattgttcgg ataacgtttt tgccagtcgg cgatcccggc
    10561 tttcatcatg tcgggatcgt tacggttatc aaaccagacg cgctgcagat ccagccagcc
    10621 ttgcagaata ttttcgtcgg cgttgatcac cagcgtattc gcctgttcct gagtcatgga
    10681 ggagagcgcc tgccaggtgg catcaatatt ctgctgtttt tctttcgcgc cgagcagcgg
    10741 ttcctgagca attaacgcgc gcagtaaatc aatggaagga cgcccctggc tggcatcgat
    10801 ttttgcctgc cagtaacgcg cttgctggtt ttgttctaaa tcggcaggtg tgattttcgc
    10861 cagcaagttt tgcgcgccag caaaatcttt ctgcgccagt ttaatctcta ccgccagcag
    10921 tgttttctcg cgacgctgag aatcgttcag ttcttgcggt agttggttaa acaactcaac
    10981 cgcctgcccg gttttacctt ctttcaccag tgcacgaatg gcgagtaatt gccagttgat
    11041 cctggtatca tcagagcttt gctgcatctg ctgaagataa aaggcagaat cagcctgcgc
    11101 cgtgccctgc atataagcag tggactgatc gggagtatgg gtgccacaac cggcgaaaat
    11161 cagggctgcc agaacaacag gcagacaacg cgcggctttc aaacgagaaa atgttgaggg
    11221 taccataatg tatccagtga tattttttta cgcaatgctc aatattaaat cggcaatacg
    11281 gacgacacaa tgaaacaaca ccaatcggcg gataattctc agggccagct ttacattgta
    11341 ccgacgccaa tcggcaatct ggcggatatc acccagcgtg cgttagaggt attacaggcc
    11401 gttgatctga ttgccgccga ggatactcgt cacaccggtt tattgctgca acattttggg
    11461 attaatgccc ggttgtttgc gctgcacgac cataacgaac aacaaaaagc cgaaacgctg
    11521 ctggcgaagc tgcaaaaagg gcagaacatt gcgctggttt ccgatgccgg aacgccgcta
    11581 attaacgatc ctggctacca tctggtgcgt acctgccgtg aagcggggat ttgcgtggtg
    11641 ccgctacccg ggccgtgtgc tgctatcact gcgttaagcg ccgcgggttt accctctgac
    11701 cgtttctgtt acgaaggctt tttacctgcc aaatcaaaag gccgccgtga tgcgctaaaa
    11761 gccattgaag cggagccgcg cacgctgatt ttttatgaat ccacccaccg tctgttagat
    11821 agcctggaag atatcgttgc ggtattaggc gaatcccgct acgtggttct ggcgcgtgag
    11881 ctgaccaaaa cctgggaaac cattcacggc gcgcccgttg gcgagctgct ggcgtgggta
    11941 aaagaagatg aaaaccgtcg caaaggcgaa atggtgctga ttatcgaagg tcataaagca
    12001 caggaagaag acttacccgc cgatgccctg cgcacgctgg cgctgctaca ggcagaactg
    12061 ccgttgaaaa aagcggcggc gctggcggca gaaattcacg gcgtgaagaa aaatgcgctg
    12121 tataagtatg cgctggagca gcaggggtaa gagtgccgaa gcgttgcgtt aatcaaaacg
    12181 caacgtcttc ttttaccgca cgatgacggt aatggtggcg gtgccctgaa attttcctgt
    12241 ttccagctca ccaccgacca gattaactgg ccaggcgcgc atgttgatgg tgccatgccc
    12301 ggaaggatca acgggaagga taccgttctg aaaaggaata tttgcaactc cgccgccgag
    12361 attttcaata cgaataccga catcgggcgc attgtctgaa cttcttcgtc tggcgaccag
    12421 gttgtactgg tctactaccc cagttgtacc gtcgatgcgc atttgcaacg agtttttaat
    12481 ctttgaagta tcaccacagt cataagtgat atcaaagtct actggagtaa aaccgtctgg
    12541 catggcattg cgggtggtaa acttctgacc attgatgaat ccaaaattaa ccttaataac
    12601 atcgccctga ttaattttgc acgactgtgg ggcggtgaga tttccctgaa tcgataactt
    12661 gactaaatca tggaagtcac ctgtagacgg gcttcccatt ccatcgaccc atgcgccttt
    12721 aatgacggca atatccgttt tcggaatggt catcgagccg agaaatggct tggtgacata
    12781 cagggtaaag gtggtatttg caccaatatt aacgggtcca taacgagacg tgtcaccctg
    12841 gcaaacaccc gtttgggttg cagcagaata tacggtgttg ctttttatct gtgtgaatcg
    12901 ggtgttaaca gaaacggtct ggtcagtcac gagtcctgga atgtcattcg cctgtaatgt
    12961 ggttttaata tccagactat cattgagttt gtaatatcct gcttgatgtc cagtggtggt
    13021 aagtacaggg ctgaccatat agacaagttt aactgcctta cccgcagagc aggtacaggc
    13081 gtattgccag ttagtgctac ttagcgtggc agaggtaaac tcccgtcctg tcacgttatc
    13141 tgtctggttc accgtaacat catcaaccac actgctgtaa tttgtcgcac cgctagtgct
    13201 gtcagctcca caccctcctg aggaggcata agcgcaggat gtggatatca acaaaagtcc
    13261 tgttataaga ggcgctcttt tcattttttt atccttaatg gcactgcgcc gtgccttgta
    13321 atacagaggt tgttggttct gaatccggtg tactaaaagc aaactgacat tgttgatctg
    13381 cctgatttcc ccagcgtaca gtccagcttt gtgatttact cgatatccca gagagatata
    13441 gtatgccgcc ctcatcgacg atactttgtt gccccgtctc atcgttgctg gcaagagcgc
    13501 caaagggcaa cggtttaccg ttgcgatcgc tgactgtaac aagtacgcga taaccgatat
    13561 tggcgttgaa acgcgccgct accattgcac ctctgttggg caccacaaac tgtgttgttt
    13621 gctcaagatc gacgttatcg ggcagctgcg tagtatctac cgagagacgg ttttcctggt
    13681 aagtcgtgag ataaggaacc actgcatagc caaagggatc agtagcaatc cccggatagt
    13741 tttgtatcct cacgccagaa gccccattag catcgataag cgcaaaagcg ttgcccagat
    13801 attgcgagag cgtcacgcca tgaggatgga taaccacgcc gccggtaacg ccgtaattgt
    13861 attggctgct gtcattaccg tagctgtatc cggcactgaa ggtgccataa ggtgaacggt
    13921 aactggcgtt caggctactg ttatggttgt tatcgtcatc cagactttgt tccaggctat
    13981 agctcaggcg accatcatcc agcagtgagc catcaagacg catttcatgt tgggttgggc
    14041 gatctttctg gctggtcatc tgataggaaa cccggctacg cggtaaccag cgttctaacg
    14101 gaatactgag attcaaagag agtgcgcggt cattattttg atccgtgtag cgactgtatt
    14161 gataattcag gctgtaacca attccccatt gctgaccgga aaccccaaca gagatattcc
    14221 tgtttttctc gttattgccc caatagtctt gctgcgaacc ggaggcatac agacttaccc
    14281 catcaaatat tgtctggctg atgttgaatt gaatttcact tttttggcga ctgttatagt
    14341 tccaattacg ggtattggct tcattaaaac taaaatagcc atcgttggta tagcgatagt
    14401 agctgacagc gatattggtg ttggtttccg ggatatcttt gatgtactgc gtacgccatt
    14461 gatagccatg aaaagagtgc tggttatcga attgggtgtc agctctgttg atatccatcg
    14521 acaacgcgcc cagtgcgcca agtgtgccgc cgataccgat ccccagcgca taataatctt
    14581 cagaaccgag caggccgcca tacagcgtaa aagtattatt caaaccatat attgccgtgg
    14641 cttcagcaaa ttcgggttcc ttgctatcac tgtttgcatc agcgcgatag cgtccagcgg
    14701 tcgcgctata ttttagatgc ccaggtcgct gcatcatggg taatgaagaa taaggctgga
    14761 taaagcgacg ttgcgtaccg tcactttctt caatcgtgac ttctaaatcg ccgctgttgg
    14821 aagaggggta gagatcgtta atttcaaagg cacccgctgg cacgttgctt tgatagatca
    14881 cataaccatt ttgcctgata gtcacgattg cactactgtt tgcgataccg cgtaccgttg
    14941 gggcaaatcc gcgctggctg tttggcaaca tattatcgtc ggaagcgagt tgcacgccag
    15001 taaaggtgta gctggaaaaa atactgccgc tggtggcgct ttctcccaga agcaactgag
    15061 acttcaacgc cttgatatca cgttgtaaat aactactgat agtgttccag cttgacgcct
    15121 gatcgttgcg tgtccacgta gaatagttac gtaatcgcca ggggccaaaa ttagcaccat
    15181 tttgcatatt caggtactgt cgttggctac gattgccctg gcggtaacgg ttatcagaac
    15241 ctgtaaacga gtagttggta aacagcgttg gtataccatc gtcccaacga gaaggggaga
    15301 cgtaacctct tgcatcacgg tacagtgcaa tttggggaat gctcaaatta agtcgctgat
    15361 gattgaaatc aaattcagca gctgtaccgg gaatgatttg ttcaagcgag ttgatcacgc
    15421 tatcgtcatc tttttcagcc agcgccggga tttcatccac cttaataccc agctcacgta
    15481 gttgttctac cgtaaactgt ggctgcagaa gttgctctgc attggcggta aatgtaattt
    15541 ttttctgtga aaccttcttt ttattcagcc agatatcaac ctgataagtt cctggtagtt
    15601 gtgcgtattt ttttgaaaaa cgtgacagat cagttgtttg cataccactt tttgtagttt
    15661 ccagcataat ggggtcgaaa taatactctt cggcgttagc ggtagtgcaa agcatgccgc
    15721 tgccagtgag caccatcggc aaacagcgtt tgatgatgag cgccaactgt ttcggtgtgc
    15781 gtttatgtcc ctggtggtgt cgttgtggca ttccctgatg acctgtgttt tttatatttg
    15841 ttattgcagt ggacgagttt ctgtcccgga actgccgcca taatcattga ttgcagccca
    15901 tttcacgttc tttccgccag atgcaggaag tggtaactgt ttatgttctc cgggcgcgat
    15961 atattcaatt ttgcttgtga gatcggtttt atcgactgcc agtccggcaa atacgacgta
    16021 atattcagtt gggttatata tattgagtga atggtcacta cgtttaaatt ccagtgatgt
    16081 ccacgcatcg tttacccgtc ccttcaggtg tgcggggcga taaaacattt tgatccgtgt
    16141 tttaaatacc agcgtcaact cgttagcatt aacatcatct gattttttct ttgctggaat
    16201 ggcacgaaca ttaatgtaga acagcgactc tttattttca ggcaggttat cagctgtttt
    16261 aataattcgc agtgatgagt catcgccagc ttcaagacga aatagcggtg gggttataat
    16321 aaatggggca cggcttgtac catctatatt atctacccat gattgcagta aataaggcgt
    16381 ttctgcgcct ttgtttgcga caggtaacgc agcctctttg cggctggcat catagatcac
    16441 tcgcgttcgc tgtaaaacga taccccctgc aagcgcctgg ccgatacaga agctacacaa
    16501 caaggttatt atcaccgcga atgttcgttt tgacatagtt atttttcaac ctgatacaac
    16561 gagaaagagg aaggaaaagg gcgttatctg aaaggtcaga taacgccgta acgtattaac
    16621 ggtaatccag cgttacgtta acgacagcgt ctgcttcccc tggggtgatt tctgatgctt
    16681 caacggttga tacgtagtaa gcgatgaggt caacattgac aataccattg gagtccgctg
    16741 ggatctgata aaccgtgtta tcattcccca tagcattcag tggcataagg tttccgcttt
    16801 ccgcatcgtc aatacggata cccacacctg ttgccgctgt gccagcaaaa gagcccactt
    16861 ttgccagatt agggaagtta gggacggtgc cattatcccg cgtttccagc cataaacgga
    16921 aagacgcttc aacgttgccg ttatcgtgct cccacccagt cactgggcag ttaactaaag
    16981 gaatagtgaa tggaattttt gggctacgat cgccaacaga acgaaactgt tcggcattat
    17041 agtgaccaag agcaacttca attttcttat cagagtcatt gatttcacaa gcggatttta
    17101 aaatctcgcc atcaaaaact atttcaccat cggttgcagc ggcattacct gcgaaaagcg
    17161 caagtaagcc cacaatagct gtttttgtaa ctttattcat ttataacctt at
//
LOCUS       DAZGAN020000104        16652 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGAN020000104 DAZGAN020000000
VERSION     DAZGAN020000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.104"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..94)
                     /locus_tag="ACHKYO_RS18845"
                     /old_locus_tag="pgaptmp_003769"
     CDS             complement(<1..94)
                     /locus_tag="ACHKYO_RS18845"
                     /old_locus_tag="pgaptmp_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430539.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMI"
     gene            complement(222..2183)
                     /gene="topB"
                     /locus_tag="ACHKYO_RS18850"
                     /old_locus_tag="pgaptmp_003770"
     CDS             complement(222..2183)
                     /gene="topB"
                     /locus_tag="ACHKYO_RS18850"
                     /old_locus_tag="pgaptmp_003770"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGU4430540.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(2188..3231)
                     /gene="selD"
                     /locus_tag="ACHKYO_RS18855"
                     /old_locus_tag="pgaptmp_003771"
     CDS             complement(2188..3231)
                     /gene="selD"
                     /locus_tag="ACHKYO_RS18855"
                     /old_locus_tag="pgaptmp_003771"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HGU4430541.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3348..3899)
                     /gene="ydjA"
                     /locus_tag="ACHKYO_RS18860"
                     /old_locus_tag="pgaptmp_003772"
     CDS             complement(3348..3899)
                     /gene="ydjA"
                     /locus_tag="ACHKYO_RS18860"
                     /old_locus_tag="pgaptmp_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HGU4430542.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4060..5916
                     /gene="sppA"
                     /locus_tag="ACHKYO_RS18865"
                     /old_locus_tag="pgaptmp_003773"
     CDS             4060..5916
                     /gene="sppA"
                     /locus_tag="ACHKYO_RS18865"
                     /old_locus_tag="pgaptmp_003773"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HGU4430543.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6083..7099
                     /gene="ansA"
                     /locus_tag="ACHKYO_RS18870"
                     /old_locus_tag="pgaptmp_003774"
     CDS             6083..7099
                     /gene="ansA"
                     /locus_tag="ACHKYO_RS18870"
                     /old_locus_tag="pgaptmp_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGU4430544.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7110..7751
                     /gene="pncA"
                     /locus_tag="ACHKYO_RS18875"
                     /old_locus_tag="pgaptmp_003775"
     CDS             7110..7751
                     /gene="pncA"
                     /locus_tag="ACHKYO_RS18875"
                     /old_locus_tag="pgaptmp_003775"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HGU4430545.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHCVEPYMPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7912..8184)
                     /gene="yeaC"
                     /locus_tag="ACHKYO_RS18880"
                     /old_locus_tag="pgaptmp_003776"
     CDS             complement(7912..8184)
                     /gene="yeaC"
                     /locus_tag="ACHKYO_RS18880"
                     /old_locus_tag="pgaptmp_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HGU4430546.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8226..8639)
                     /gene="msrB"
                     /locus_tag="ACHKYO_RS18885"
                     /old_locus_tag="pgaptmp_003777"
     CDS             complement(8226..8639)
                     /gene="msrB"
                     /locus_tag="ACHKYO_RS18885"
                     /old_locus_tag="pgaptmp_003777"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGU4430547.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8981..9976
                     /gene="gapA"
                     /locus_tag="ACHKYO_RS18890"
                     /old_locus_tag="pgaptmp_003778"
     CDS             8981..9976
                     /gene="gapA"
                     /locus_tag="ACHKYO_RS18890"
                     /old_locus_tag="pgaptmp_003778"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU4430548.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            10060..10944
                     /gene="yeaD"
                     /locus_tag="ACHKYO_RS18895"
                     /old_locus_tag="pgaptmp_003779"
     CDS             10060..10944
                     /gene="yeaD"
                     /locus_tag="ACHKYO_RS18895"
                     /old_locus_tag="pgaptmp_003779"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HGU4430549.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVTLTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(10995..11849)
                     /gene="yeaE"
                     /locus_tag="ACHKYO_RS18900"
                     /old_locus_tag="pgaptmp_003780"
     CDS             complement(10995..11849)
                     /gene="yeaE"
                     /locus_tag="ACHKYO_RS18900"
                     /old_locus_tag="pgaptmp_003780"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HGU4430550.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTDLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELIMLDKAYPAPK
                     GKTALDMV"
     gene            complement(11939..12685)
                     /gene="mipA"
                     /locus_tag="ACHKYO_RS18905"
                     /old_locus_tag="pgaptmp_003781"
     CDS             complement(11939..12685)
                     /gene="mipA"
                     /locus_tag="ACHKYO_RS18905"
                     /old_locus_tag="pgaptmp_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HGU4430551.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLCDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            13121..15055
                     /gene="yeaG"
                     /locus_tag="ACHKYO_RS18910"
                     /old_locus_tag="pgaptmp_003782"
     CDS             13121..15055
                     /gene="yeaG"
                     /locus_tag="ACHKYO_RS18910"
                     /old_locus_tag="pgaptmp_003782"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HGU4430552.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            15168..16451
                     /gene="yeaH"
                     /locus_tag="ACHKYO_RS18915"
                     /old_locus_tag="pgaptmp_003783"
     CDS             15168..16451
                     /gene="yeaH"
                     /locus_tag="ACHKYO_RS18915"
                     /old_locus_tag="pgaptmp_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HGU4430553.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         4114 a         4294 c         4083 g         4161 t
ORIGIN      
        1 taatcatccg ttcaaaatta ctattaaata tttcccagcc attaaaagaa tacttctcgc
       61 tcccaggatg gttttgtaat aaaacttttt tcatcacatt ccctgttatt acatacttta
      121 gaggcgataa ttttatccat gcaaaaaaaa tatccaacga attttcttga tccgctggat
      181 atttcagaat atgactcgaa tagcacgaaa gattcgctcg cttacgctat cgccccgctt
      241 ccggcttcat ctgctggcgg actttttttc gcactacgtt tacgcggtgc agcctttttc
      301 ttatcagcac tgccaccact gcccggagcc acaatgccgc gaaactgccg caccggagta
      361 cgtttggctt gatcaataag ctgatatagc gtccccacca gcggctgcat aaagtcctga
      421 tagcgacact gcttttcgct gatttgcgtc agcaccgatt cccagtgcgc ggtcatgtcc
      481 ggtcgcgtag ccatttctgg cagcgaatgg aataacgctt ttccggcgtc ggtggagtgg
      541 atatagcgcc cttttttggt gaggaaacca cgcttgaaca acagttcaat aatcccggca
      601 cgcgttgctt ctgtccccag accatcggtc gcacgaagga tctttttcag atctttgtcc
      661 tgcacaaagc gcgcaatccc ggtcatcgcc gaaagcagtg ttgcatcggt aaaatggcgc
      721 ggcggctggg tttgccgctc taccacttca cctttttcac acagcaactc atcgcctttc
      781 gccaccacag gcaatggcgt gccgtcgttt tcttcatcgc gctctttgct gcctaacagc
      841 gcgcgccaac ctgcttcagc aagaaaacgc gctttagcga caaatttgcc tttggcaatg
      901 tccagttcga taacacactt gcggaacacc gcatccgggc agaactgcat cagatactga
      961 cgggcaatca ggttatagac cttcgcttcg ttctccgtca ggttgatcgc agaactcctt
     1021 gcggtcggaa tgatggcgtg gtgcgcatcg acctttttgt catcccaaca gcggttgcgt
     1081 atatctggat ctaccactga ctgcggcaac agatccggtg catgaacgct gatggcattc
     1141 atcaccgcgt ggcgtccggc aaaatgttct tctggcaaat agcgacaatc ggaacgcgga
     1201 taagtgatta gcttgtgcgt ttcgtacagc ttctggcaga tatcaagcac gttctgcgcg
     1261 ctcagaccga agcgttttgc cgcttcaatc tgcaacgccg aaagcgaaaa cggcagcggc
     1321 gcggattctg attcccgttt atcgttatag ctggtgacaa tagccggttg accactaatg
     1381 cggttaacca catgctccgc cagtggacga tgtaacaagc gcccttcttc atcctggtac
     1441 ggttcacacg cttcgctcgg ttgccagata gcggtaaacc gctcatcggc aggtgtcacg
     1501 atatgcgctt tgacttcaaa gaagtctttc gccacgaagt tttcaatctc ttcatcgcgg
     1561 cgcaccacca gcccgagcac gggtgtctgc acgcgtccca cggaaagtac gccctgataa
     1621 ccggcattgc gaccgagaat ggtatacgca cgggtcatat tgatgccgta cagccagtcg
     1681 gcacgcgctc gcgccagcgc agaaacgcac agcggtacaa actcactgtt ggaacgaaga
     1741 cggtcgatcg cccgctcaac cgcctgcggg ttcaggtcgt ttatcaagca acgctgcacc
     1801 tgctggcgct tttccggtgc cagttgtaga tagtccagca cttcatccac cagcagttgc
     1861 ccttcgcgat ccgggtcccc ggcgtgaacg atttcgctgg cttcatgcag gaaccgtttg
     1921 atgacgttaa gttgtttggt cacggagggt cggggctgta attgccactt ttccgggaca
     1981 atcggcaaat ccgcaagatt ccagcgcgca tagcggctgt cgtaggcgtc tggctgcgcc
     2041 tgctcaagca ggtgaccgat acaccaggtc accacctgac cattaccgca ctcgataaag
     2101 ccatcgcctt tacggtgcgg tttgggcagg acatcagcaa tggcgcgcgc cagactcggt
     2161 ttttcggcaa taaacaaccg cattgaatta acggatctca accatggcac gaccgccgcg
     2221 cgctggcacc agttcgccaa tcgccgtcag ttcaatgcca aactcggcgg ctgtagcttt
     2281 aacctcattt tctgcttccg gcatgaccgc cagcagcaaa ccgccagaag tttgcggatc
     2341 gcacagcaga tcgcgcactt cacgcggcat ttcacccatc agatgaccgt agctggcaaa
     2401 gttacgttcg gtgccgcctg gcactgcgcc caacttaatg tactcttcaa cccccggcaa
     2461 tttcgggatc gcgtcatagt caacgcgcgc ctgcacacct gcgccctgac acatttcgct
     2521 caaatggccc agcagaccaa agcccgtaac gtcggtcatc gcttttacgc cttcgatgtt
     2581 ggcaaaggac gcgcctgcga tattcatccg gcacatcact tctgtcgcca gtccctgatg
     2641 ttctggtttc agcagtgatt ttttctcagc cgtggtaaga acgccgatcc ccagcggttt
     2701 cgtcaggaac agtttgcatc cggcttgtgc ggtactgttt ttcttcaccc gctcggtcgg
     2761 tacgatcccc gttaccgcca gaccaaaaat cggctccggc gcatcgatgg agtgaccgcc
     2821 cgccagcgca atacccgcct gacggcatgc atagcgtcca ccttcggtta cttcgcgggc
     2881 aatttccggg gaaagtttgt taatcggcca gccgaggatc gcaatcgcca taatcggttt
     2941 gccccccatc gcgaagatat cgctgatggc gttagtcgcc gcaatgcggc caaaatcgaa
     3001 aggattatca acgatcggca taaagaagtc ggtggtactg ataacgctgg tgccattgcc
     3061 cagatcgtac accgccgcat cgtcgcgggt ctcattaccc acaagcaaat tcggatcaac
     3121 aaacttcgcc tgctcactgt gcaggatggt ttccaacact tttggggaaa ttttacagcc
     3181 gcaaccagct ccgtggctgt attgggtcaa acgaatcgag ttctcgctca tggacatctc
     3241 ctgtcaatgc aatccgggta tggtaaccct cattccgtga agtgataagt gagagtgtct
     3301 gaattcctgc gcctttgctc acaatccaga cagtttcgcg acaattatca gaaataagtt
     3361 acaaacggcg tcgggtccgg gacgttaatc gacgtagatg ctttcaactg cggcgtgccg
     3421 aggtagagaa aaccgacaat tttatcctgc tcacggcaac cgaatgcttc acgcactacg
     3481 ggactttcag ttaatgcgcc actgcgccag atgccgccaa atccttgggc aacggctgcc
     3541 atttgcatcg ccatgaccgc gcatccggca gacatttcct gttcccatcg cgggacttta
     3601 tgattttctt cgcatttcgc caccaccgtg atgatgagcg gtgcgcggaa cggcgcatta
     3661 cgggctttgt cgatagcttt gtcatcacta ccggcagcaa tcgccccctg ttccagtacg
     3721 gcgctgaaac gctcgcgccc ttccccttca atcacaaaaa aatgccacgg ttgcatggac
     3781 ttatggtccg gcgcacgcat acccgcacgc aggatgtttt gcagttgttc acccgttggc
     3841 gcgggttcag ccaggcggga ggcgctacgg cgattgatca atagttcgag tgcatccatt
     3901 tgattaactc ctgtcgtgat atttattcac aaaattaaca cgagagtgga ttttgttaca
     3961 gcacagtccg caattcctgc tgacaagtgc cggttgggtc attacgataa ccacatctat
     4021 tgcgcctgtg acaggtgtga ccttaagttg ggagaataca tgcgaaccct ttggcgattt
     4081 attgccggat tttttaaatg gacgtggcgt ctgctgaatt tcgtccgtga aatggtactt
     4141 aacctgttct ttattttcct cgtactggtt ggtgtgggga tctggatgca ggtcagtggt
     4201 ggtgattcga aagaaacggc cagtcgtggc gcactgctgc tggacatttc tggtgtgatc
     4261 gtcgataaac ccgacagttc tcagcggttt agtaaattaa gccgccagct gcttggtgcc
     4321 agttccgatc gtctgcagga aaactcactg tttgatatcg tcaacaccat tcgccaggcg
     4381 aaggacgacc gcaatatcac cggtatcgtg atggatctga aaaacttcgc aggcggcgac
     4441 cagccgtcta tgcagtacat cggcaaagcc ctgaaagagt ttcgtgacag cgggaaaccg
     4501 gtttatgccg ttggcgagaa ctacagccag gggcaatatt atctcgccag tttcgccaat
     4561 aaaatttggc tgtcaccgca aggcgtggtt gatctgcacg gttttgctac caacggtctg
     4621 tactacaaat cgttgctgga taagctgaaa gtttccaccc atgtgttccg cgtgggtacg
     4681 tataaatctg ccgttgaacc gtttattcgt gatgatatgt caccggcagc ccgcgaagct
     4741 gacagccgct ggattggtga gctgtggcaa aactatctga atactgttgc cgctaaccgg
     4801 cagatccctg ctcagcaggt attccctggc gcgcaagggt tgcttgaggg tttaaccaaa
     4861 accggtggcg ataccgcgaa atatgcactg gaaaacaagc tggtcgatgc actggcatcg
     4921 agtgccgaaa tcgaaaaagc tctgaccaaa gagttcggct ggagtaagac tgataaaaat
     4981 tatcgcgcca tcagttatta cgattacgca ttgaaaacgc cggcagatac cggtgacagc
     5041 atcggtgtcg tcttcgctaa tggcgcaatt atggatggcg aggaaactca ggggaatgtt
     5101 ggcggtgata ccactgcggc acaaatccgc gacgctcgcc ttgacccgaa agtgaaagcg
     5161 attgtcctgc gtgttaatag cccaggcggc agcgttaccg cgtctgaagt gattcgcgct
     5221 gaactggcag cagcccgggc agcgggtaag cctgtggttg tatcgatggg cggcatggcg
     5281 gcatctggtg gttactggat ttccacgcca gctaattaca ttgtggctaa ccccagcacc
     5341 ctgaccggtt ctatcggtat cttcggcgtg atcaccaccg tagaaaatag tctggattcg
     5401 attggtgttc atactgatgg tgtctcaact tcaccgctgg cggatgtttc tatcaccagg
     5461 gcactgccgc cggaagcgca gcagatgatg caattaagca ttgagaatgg ctataaacgc
     5521 tttatcacgc tggttgctga tgcgcgtcat tcgacgccgg agcaaattga taaaatcgcc
     5581 cagggccacg tctggaccgg tcaggatgca aaagctaacg ggctggtcga tagtctcggg
     5641 gatttcgatg atgcggttgc caaagcagca gagctggcaa aagtgaaaca gtggcatctg
     5701 gaatactacg ttgatgaacc gaccttcttc gacaaagtga tggacaacat gtctggttct
     5761 gtccgggcaa tgttgccaga tgcgttccag gccatgttac ctgcaccgct tgcctcggta
     5821 gcctctaccg ttaaaagtga aagcgacaag ctggccgcgt ttaacgaccc acaaaaccgt
     5881 tatgcgtttt gcctgacctg cgccaacgtg cgttaagtct tgtactgagt ggccgacaga
     5941 tcgtcggcca cattattttt tacctcgacg aatcctcttc ccgctgtctc gacccatatt
     6001 tccttatccg catagtatca ggtgcgctcc tcctgcctca cgtatatact tttgctcttt
     6061 cgatatcatt catatcaata tcatgcaaaa gaaatcaatt tacgtcgcct acacgggcgg
     6121 gaccatcggg atgcagcgtt ccgagcaggg ttatattccg gtgtcaggtc atctacaacg
     6181 ccaactggcg ctgatgccgg aattccatcg cccggagatg ccagatttca ccattcatga
     6241 atatacgccg ttgatggatt cttctgatat gacgccggaa gactggcagc atattgctga
     6301 agatattaaa gcgcactatg acgactatga tggttttgtc attctacacg gtaccgacac
     6361 gatggcgtat accgcctctg cgctgtcgtt catgctcgag aatctcggta aaccggtcat
     6421 tgtgacaggg tcacaaatcc cgctggctga gttacgctct gacggacaaa ttaatctgct
     6481 gaatgcgttg tacgttgcgg cgaattatcc gatcaacgaa gtaacgctct ttttcaataa
     6541 ccgattgtat cgcggcaacc gcactaccaa agcccatgcc gatggttttg atgcgtttgc
     6601 ctctccaaac cttcctccgt tactggaagc tggtatccat attcgtcgtt tgaatacgcc
     6661 acccgccccg cacggtgaag gggaattaat cgttcatcca atcacgccac aaccaattgg
     6721 cgtagtgacg atttatccgg ggatttctgc tgatgtcgtg cgcaattttc tgcgccaacc
     6781 ggtgaaagca ttgattctgc gctcctatgg cgtgggtaat gcgccacaaa acaaagcctt
     6841 cctgcaggaa ttacaagaag ccagcgatcg cggtattgtg gtggttaacc tgacacaatg
     6901 tatgtccggt aaagtgaaca tgggtggtta tgccaccggt aacgccctcg cccatgccgg
     6961 cgtaattggc ggtgcagata tgactgtaga agctacgcta accaaactgc attacctgct
     7021 gagccaggaa ctggatactg aaaccattcg caaggccatg agccaaaacc tgcgcggcga
     7081 actgacgccg gatgattaag gagactgcaa tgccccctcg cgccctgtta ctggtcgatt
     7141 tacaaaatga tttctgtgct ggtggcgcgc tcgccgtgcc ggaaggtgac agtacggtgg
     7201 atgtcgctaa ccgccttatt gactggtgcc agtcgcgcgg tgaagcggtt atcgccagtc
     7261 aggactggca cccggcgaat cacggcagtt ttgccagtca gcactgtgta gagccttata
     7321 tgccaggtca actcgacggt ttgccacaaa ccttctggcc agatcactgt gtgcagaaca
     7381 gtgaaggcgc acaattacat ccgttactga accaaaaagc gatcgcagcg gtgttccata
     7441 aaggcgaaaa tcctttagtt gacagttaca gtgccttttt tgataacggc cgtcggcaga
     7501 aaacctctct cgatgactgg ttacgcgatc atgaaatcga tgaattgatc gttatgggcc
     7561 tggctactga ctattgcgtg aagtttaccg tgctggacgc gttacagtta ggttataaag
     7621 taaacgtgat taccgatggt tgtcgtggcg tgaatatcca gccccaggac agtgcgcacg
     7681 cgtttatgga gatgtcagca gctggggcaa cgctatatac gctggcagac tgggaagaga
     7741 cgcaggggta atattagttg aattcatctc tggcctacat ttcctcaatg acattgtagg
     7801 ccagataata cttgtcagtg ttgcatttaa cctacttgca gctaaaacac gacgtcctaa
     7861 caagtatatt tgccctccct taataacagg cttaaatact agataactca ttcacttaaa
     7921 catagcaata ggctttgctg agataccaaa atcttctttt aactgctgtt tgctcttcat
     7981 gaccatctga ccatttgtgt caattgtcat atgctgtgct tcggtattat ggcgggcttg
     8041 ccacagcatc accagttgca ggcaattctc tttttgttcc tcggtcaacg caacgccatc
     8101 aggccatttc cccagttcaa cggcggtcga caaacgctgg tatacctcag gcgtcatgct
     8161 gttgataatg tcatcaagat tcataatttt tccgctcctg tggaataagt tgctgaatcg
     8221 ttttttcaac cgttgatttc ttcgccgttt tcgccatcag tgaagcgtaa agaagcagag
     8281 ttaacacaat aacgttcgcc tgttggttgc ggcccgtcgg gaaagacatg ccccagatgg
     8341 gcatcacagt taccgcaacg aatttctatg cgctgcattc catgtgacaa gtctttgata
     8401 taacgaatgg attcttcact caccggttcg tagaaactgg gccagccaca gccggaatca
     8461 tacttggttt cggaatgaaa cagcggggca tcgcagatca aacagtgata tacgccgtca
     8521 cgcttgttat gcagtaaacg acccgtaaat ggcggttctg tcccatgatt ctgcgtcacg
     8581 taaaactgca tctcggacaa attttttttc agttcttctg ccgaaggttt attagccatt
     8641 tgctcacatc tcactttaat cgtgctcaca ttacgtgact gattctaaca aaacattaac
     8701 accaactggc aaaattttgt cctaaacttg atctcgacga aatggctgca cctaaatcgt
     8761 gatgaaaatc acatttttat cgtaattgcc ctttaaaatt cggggcgccg accccatgtg
     8821 gtctcaagcc caaaggaaga gtgaggcgag tcagtcgcgt aatgcttagg cacaggattg
     8881 atttgtcgca atgattgaca cgattccgct tgacgctgcg taaggttttt gtaattttac
     8941 aggcaacctt ttattcacta acaaatagct ggtggaatat atgactatca aagtaggtat
     9001 caacggtttt ggccgtatcg gtcgcattgt tttccgtgct gctcagaaac gttctgacat
     9061 cgagatcgtt gcaatcaacg acctgttaga cgctgattac atggcataca tgctgaaata
     9121 tgactccact cacggccgtt tcgacggtac cgttgaagtg aaagacggtc atctgatcgt
     9181 taacggtaaa aaaatccgtg ttaccgctga acgtgatccg gctaacctga aatgggacga
     9241 agttggtgtt gacgttgtcg ctgaagcaac tggtctgttc ctgactgacg aaactgctcg
     9301 taaacacatc actgctggtg cgaagaaagt ggttatgact ggtccgtcta aagacaacac
     9361 tccgatgttc gttaaaggcg ctaacttcga caaatatgct ggccaggaca tcgtttccaa
     9421 cgcttcctgc accaccaact gcctggctcc gctggctaaa gttatcaacg ataacttcgg
     9481 catcatcgaa ggtctgatga ccaccgttca cgctactacc gctactcaga aaaccgttga
     9541 tggcccgtct cacaaagact ggcgcggcgg ccgcggcgct tcccagaaca tcatcccgtc
     9601 ctctaccggt gctgctaaag ctgtaggtaa agtactgcca gaactgaatg gcaaactgac
     9661 tggtatggcg ttccgcgttc cgaccccgaa cgtatccgta gttgacctga ccgttcgtct
     9721 ggaaaaagct gcaacttacg agcagatcaa agctgccgtt aaagctgctg ctgaaggcga
     9781 aatgaaaggc gttctgggct acaccgaaga tgacgtagta tctaccgatt tcaacggcga
     9841 agtttgcact tccgtgttcg atgctaaagc tggtatcgct ctgaacgaca acttcgtgaa
     9901 actggtatct tggtacgaca acgaaaccgg ttactccaac aaagttctgg acctgatcgc
     9961 tcacatctcc aaataagttg agatgacact gtgatctaaa aagagcgact tcggtcgctc
    10021 ttttttttac ctgataaaat gaagttaaag gactgcgtca tgattaagaa aatttttgcc
    10081 cttccggtca tcgaacaaat ctcccctgtc ctctcccgtc gtaaactgga tgaactggac
    10141 ctcattgtgg tcgatcatcc ccaggtaaaa gcctcttttg cattacaggg cgcacacctt
    10201 ctctcgtgga aacctgcggg tgaagaagaa gttctgtggt tgagcaacaa cacaccgttc
    10261 aaaaatggcg tcgctattcg cggtggcgta ccggtttgct ggccgtggtt tggtccggcg
    10321 gcacaacaag gtctgcctgc gcacggtttt gcccgcaacc tgccgtggac gctgaaatca
    10381 catcgtgaag atgctgatgg cgtaacgctg acttttgaat tgacgcaaag cgaagagacg
    10441 aaaaaattct ggccgcacga ctttacgctg ttagcgcatt tccgcgtggg taaaacctgt
    10501 gaaatcgatc ttgaatcaca tggcgaattt gaaaccacct ctgccctgca tacctacttt
    10561 aacgtgggtg atatcgctaa ggtaagcgtc agtgggctgg gcgatcgctt cattgataaa
    10621 gtaaatgacg cgaaagaaga tgtactgacc gacggtattc agaccttccc tgaccgtacc
    10681 gatcgcgtgt atctgaatcc gcaagattgc agcgtgatta atgatgaagc gctgaatcgt
    10741 attatcgccg taggccacca gcatcatctg aacgttgtcg gctggaaccc gggaccggcg
    10801 ctttcagtta gcatgggcga catgccggat gatggctaca aaacatttgt ttgtgtagaa
    10861 acggcttacg cttcagaaac gcaaaaagtg accaaagaga aacctgcaca tctggcgcaa
    10921 tccattcgcg ttgcgaaacg ttaatttgcg ttaatgttgt gtgccgggtg cgatgcatcc
    10981 ggcacgtatc aacatcacac catatccagc gcagtttttc cttttggtgc cggatatgct
    11041 ttatccagca tgattaattc cgctgacgaa agttcgacct caagcacagc cgcattctgt
    11101 tggacatggg caatcgtggc cgcttttgga atcgccatca caccctgatg gctgattacc
    11161 cacgccaaca atacttgtgc cgcgctgata ttgtgagcat gtgcaatttc gttgactacc
    11221 gcgtttttta acagtccatt gcgcaaccgc ccggcctggg ctaatggact gtaagccatt
    11281 accggcatct gctgttgctg gcaccagggg agtagatcgt actcaatccc tcgtgaaccg
    11341 agatggtaaa gcacctgatt agtggcacac tgatttcccc ccggcagctg ccagagttcc
    11401 tgcatatcag cataatcaag gttagaaacg ccccagcggc ggatttttcc ctgggcgatc
    11461 aatttttcca tcgctgccac agtctcttca aaagcgaaac tgccagacca gtgtaataag
    11521 tagagatcga gataatcagt attgagacgg cgtaaactgg cttcgcatgc atttatcgct
    11581 ttttgcccgc cagcattcca cggatagact ttagagacga gaaagacgtt atcgcgcaga
    11641 tcggttaatg cttccccaac caccttttcg gcaccgccat cggcatacat ttcggcggta
    11701 tcaatgaggg ttaaaccgag ctcaatgccc gcgcgtagtg cagcaacttc tgttttgcgc
    11761 tgactggcat cttcgcccat ataccatgtt ccctgcccta tggctggcag tgagacatcg
    11821 ccactaaatt gaatcatttt ttgttgcatt gtttcctccc aggtattgca ccaccgtaat
    11881 gcaaaacagg gcgtgacgcc ctgtttttat gcacaaaatg ccctggaaag atgcattatc
    11941 agaatttgta ggtgatcccg gtagaaatca ggccagtcca ggatttatcc accatcgggc
    12001 tgtcagtaac ttcatcagac agacgggtat agcgcgcggt accgtaaaca ctccagtcgc
    12061 agaggaagtt gtagctggcg ctcagctcca ggtaagggct ccagctgtcg ttcgggttat
    12121 agccacgcag accgctgcga gcggactctt tgcgcgatac gccataatag tattcgttct
    12181 ggttttcgct gttccactgc acaccaatac ccggagtcac ggtcaggcca ccgttggtgt
    12241 aacgatacaa ccaggccata tcccagacaa tgccgttgct gttatccagg gtatcgccag
    12301 ccagggtggt acgcaggtaa ccgtactggg taaagtgagc ataagacaga ccagccatca
    12361 tggtgctctt acggtcatcc aggtgacgca tttggtgatc gccactgtct ttcgctttga
    12421 agtaaagcgg cgaccagtaa gcggtaattg aaagtttatc cgttgcgtca ttccacaggt
    12481 agtaaccacc acctaagcca cggaaccaga agttatcgcc ttcatagttg attaccggta
    12541 ctgggtaaac atcggtatcg taatctttat atgggtgctc aacgacacct acgcctgcgc
    12601 ccagggaaaa tttaccttca gcgtgcgcta cgcctgcaga cgttgcgata agcactccaa
    12661 gtgccagaag tttgagtttg gtcacaatta atcattcctt aaacaaatgt ttagcgggcg
    12721 acaaagttta cccgtcaata catcgaaacc caaccttttt acgttttcat tttttaaagt
    12781 aactgtttaa ttttcctgac gcggatgaca ccgcgcttac agccaaatga atttagcgtt
    12841 actggctacc ctggtcttta cattaattat gcaaaattta tggatgagtt gttgatatgc
    12901 cattgaaatt aagaaagccg tgcaggcaag ttttccattt gccatctacg cttaattttg
    12961 aaggtgtatc accgggcacg ttgttctcat cgtcgataaa atggcatgag agttgctgtg
    13021 ttttagcaag agacgtcgtt cagtttacct cttccgggag cctctactat tcatatgaac
    13081 ggctcttaac ctgtgctaaa aaacgaaagg acggcatacc atgaatatat tcgatcacta
    13141 tcgccagcga tatgaagctg ccaaggacga agagttcaca ctgcaggagt ttcttaccac
    13201 ttgtcggcaa gatcgcagtg cttatgccaa cgcggctgag cggctattga tggctatcgg
    13261 tgagcctgtc atggtcgata cagcccagga acccagactt tctcgactct tttctaaccg
    13321 ggtcattgca cgttatccgg catttgaaga gttttacggc atggaagacg cgattgaaca
    13381 gattgtctct tatctgaaac acgcggctca ggggctggaa gagaagaaac aaatcctgta
    13441 tctgctgggg cctgtgggtg ggggtaaatc atcgcttgct gagcgactga aatcattaat
    13501 gcagctcgta ccgatttatg tattgagcgc caacggtgag cgtagcccgg tcaacgatca
    13561 tccgttctgt cttttcaatc cgcaggaaga tgctcagatt ctggaaaaag agtatggcat
    13621 tcctcgccgt tatctcggca ccatcatgtc gccatgggcg gcaaaacgcc tgcatgaatt
    13681 tggtggcgat atcactaagt tccgggtagt gaaggtctgg ccgtcaattc tgcaacaaat
    13741 tgctatcgcc aaaacggaac ccggtgatga gaacaaccag gacatctccg cgctggttgg
    13801 gaaagtcgat attcgtaaac tcgaacacta cgcgcagaat gacccggacg cctacggcta
    13861 ttccggtgcg ctgtgccgcg ccaaccaagg gatcatggaa tttgtcgaga tgtttaaagc
    13921 accgataaaa gtgctgcatc cgctgttaac cgccactcag gaaggtaact acaacgggac
    13981 ggaaggtatc tccgccctgc cgttcaacgg gattattctc gcccactcga acgaatccga
    14041 atgggtcact ttccgtaata acaaaaacaa cgaagccttc ctcgaccgtg tttacatcgt
    14101 gaaggtgccg tattgcttac gcatttccga agagatcaaa atctacgaga aattgcttaa
    14161 tcacagtgaa ttgactcacg ccccctgcgc ccctggcacg ctggaaacgc tgtcacgttt
    14221 ttccattctt tcgcgcctta aagagccaga aaactccagc atttattcaa agatgcgggt
    14281 ttatgatggc gaaagtctga aagacaccga tcccaaagcc aagtcgtatc aggaatatcg
    14341 tgactacgcc ggtgtcgatg aagggatgaa cggtctgtcg acgcgttttg cgtttaagat
    14401 cctctcccgc gtgttcaact tcgatcatgt agaagtggcg gcaaacccgg tccatctgtt
    14461 ctacgtactg gaacagcaga tcgagcgcga gcagttccca caagagcagg cagaacgcta
    14521 tctggagttc ctgaaaggtt atctgatccc gaaatatgcc gagtttatcg gcaaagagat
    14581 ccagacggcc taccttgaat cctattccga atatgggcaa aacattttcg accgttatgt
    14641 tacctacgcg gatttctgga ttcaggatca ggagtatcgc gatccggata ccgggcagct
    14701 gtttgaccgc gagtctctta acgccgagct ggagaaaatc gagaaaccgg cggggatcag
    14761 taatccaaaa gatttccgca acgagattgt taacttcgta ctgcgcgcca gagcgaataa
    14821 cagcggacgc aatccgaact ggaccagcta tgaaaaactg cgcacggtta tcgagaagaa
    14881 aatgttctcc aataccgagg agctgttgcc ggttatctcg tttaacgcca aaacgtcaac
    14941 cgacgagcag aagaaacacg acgactttgt tgaccgtatg atggaaaaag gctacacccg
    15001 taaacaggtg cgtttactgt gcgaatggta tttgcgcgta cgtaaatcgt cttaacaacc
    15061 ctggcccggt acacatgtta ccgggcctac aacgacagcg aaccgtgggc ctgagaagcg
    15121 gcaacacagg cgtagcatac agttggcaaa tgtagtacgg ggggcatatg acctggttta
    15181 ttgaccggcg tctgaacggc aaaaacaaaa gcatggtgaa tcgccagcgt tttttacgcc
    15241 gttataaagc gcaaattaaa cagtcgatct ccgaggccat taataagcgt tcggtgactg
    15301 acgtcgacag cggcgaatcc gtatccattc ccacggaaga tattagcgaa ccgatgtttc
    15361 atcaggggcg tggcggtctg cgccaccgcg tgcatccggg caatgaccat ttcgtccaga
    15421 acgaccgaat tgaacgtccc cagggtggcg gcggaggttc cggcagtggt cagggccagg
    15481 ccagccagga tggtgaaggt caggatgaat ttgtctttca gatttcgaaa gatgagtatc
    15541 ttgatttgct ctttgaagat ttggccttac cgaatctgaa acaaaaccaa caacgccagc
    15601 tgaccgaata taaaacgcat cgggcgggtt ataccgctaa cggcgttccg gcaaatatca
    15661 gcgttgtgcg ctccttgcag aactcactgg cgcgacgcac agccatgacg gcaggcaagc
    15721 ggcgggaact tcatgcactg gaagagaatt tggccatcat cagcaacagt gaacctgcgc
    15781 agctgctgga agaggaacgt ctgcgcaaag aaattgcaga attacgtgcc aaaattgaac
    15841 gcgtcccttt tattgacacc ttcgatttac gttacaagaa ctacgagaag cggcccgatc
    15901 cctccagcca ggcagtgatg ttttgcctga tggacgtttc cggttcaatg gatcaatcca
    15961 ctaaagatat ggctaagcgt ttttatattc tgctgtatct gttcctcagc agaacgtata
    16021 agaacgtgga agtcgtatac atccgccatc atacccaggc gaaagaagtc gatgaacatg
    16081 agtttttcta ctcgcaggaa acaggcggca ccatcgtttc cagcgccctg aaactgatgg
    16141 atgaggtagt gaaagagcgt tataacccgg cacagtggaa tatttacgct gcacaagcat
    16201 cggacggcga taactgggcc gatgactctc cgctttgcca tgaaatcctg gcgaaaaaat
    16261 tattacctgt tgttcgttat tacagctata tcgaaattac ccgtcgtgca catcagacat
    16321 tgtggcgaga atatgagcat ctgcaatcta ctttcgacaa ctttgcgatg cagcacatcc
    16381 gcgaccagga tgatatttat ccggtgttcc gtgaactgtt tcataaacaa aatgcaacag
    16441 ctaaagacta aaactatcag ccaggtcatt atcgcctggc tgatttttag cttactgtaa
    16501 attatctcat ttattacata cattagctta caatcgcttt aaatatgaca gcataacctt
    16561 tacataattt agttccagaa aacaatcatt cggaaaaatg attcagtcaa cacgtatttc
    16621 catggggtta ttctttaaat attttttatc gt
//
LOCUS       DAZGAN020000105        16546 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGAN020000105 DAZGAN020000000
VERSION     DAZGAN020000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.105"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            116..667
                     /gene="ygiL"
                     /locus_tag="ACHKYO_RS18920"
                     /old_locus_tag="pgaptmp_003784"
     CDS             116..667
                     /gene="ygiL"
                     /locus_tag="ACHKYO_RS18920"
                     /old_locus_tag="pgaptmp_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGU4430554.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            727..3231
                     /locus_tag="ACHKYO_RS18925"
                     /old_locus_tag="pgaptmp_003785"
     CDS             727..3231
                     /locus_tag="ACHKYO_RS18925"
                     /old_locus_tag="pgaptmp_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4430555.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSFSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3247..3996
                     /gene="yqiH"
                     /locus_tag="ACHKYO_RS18930"
                     /old_locus_tag="pgaptmp_003786"
     CDS             3247..3996
                     /gene="yqiH"
                     /locus_tag="ACHKYO_RS18930"
                     /old_locus_tag="pgaptmp_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4430556.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNQSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3998..5062
                     /gene="yqiI"
                     /locus_tag="ACHKYO_RS18935"
                     /old_locus_tag="pgaptmp_003787"
     CDS             3998..5062
                     /gene="yqiI"
                     /locus_tag="ACHKYO_RS18935"
                     /old_locus_tag="pgaptmp_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4430557.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNMVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(5105..5305)
                     /gene="glgS"
                     /locus_tag="ACHKYO_RS18940"
                     /old_locus_tag="pgaptmp_003788"
     CDS             complement(5105..5305)
                     /gene="glgS"
                     /locus_tag="ACHKYO_RS18940"
                     /old_locus_tag="pgaptmp_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HGU4430558.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            5574..6203
                     /gene="yqiJ"
                     /locus_tag="ACHKYO_RS18945"
                     /old_locus_tag="pgaptmp_003789"
     CDS             5574..6203
                     /gene="yqiJ"
                     /locus_tag="ACHKYO_RS18945"
                     /old_locus_tag="pgaptmp_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGU4430559.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            6230..7891
                     /gene="yqiK"
                     /locus_tag="ACHKYO_RS18950"
                     /old_locus_tag="pgaptmp_003790"
     CDS             6230..7891
                     /gene="yqiK"
                     /locus_tag="ACHKYO_RS18950"
                     /old_locus_tag="pgaptmp_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HGU4430560.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     GRRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            8130..8189
                     /locus_tag="ACHKYO_RS18955"
                     /old_locus_tag="pgaptmp_003791"
     CDS             8130..8189
                     /locus_tag="ACHKYO_RS18955"
                     /old_locus_tag="pgaptmp_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4430561.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            8506..8565
                     /gene="ibsE"
                     /locus_tag="ACHKYO_RS18960"
                     /old_locus_tag="pgaptmp_003792"
     CDS             8506..8565
                     /gene="ibsE"
                     /locus_tag="ACHKYO_RS18960"
                     /old_locus_tag="pgaptmp_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HGU4430562.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(8685..10118)
                     /gene="hldE"
                     /locus_tag="ACHKYO_RS18965"
                     /old_locus_tag="pgaptmp_003793"
     CDS             complement(8685..10118)
                     /gene="hldE"
                     /locus_tag="ACHKYO_RS18965"
                     /old_locus_tag="pgaptmp_003793"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HGU4430563.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(10166..13006)
                     /gene="glnE"
                     /locus_tag="ACHKYO_RS18970"
                     /old_locus_tag="pgaptmp_003794"
     CDS             complement(10166..13006)
                     /gene="glnE"
                     /locus_tag="ACHKYO_RS18970"
                     /old_locus_tag="pgaptmp_003794"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HGU4430564.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFRAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(13029..14330)
                     /gene="ygiF"
                     /locus_tag="ACHKYO_RS18975"
                     /old_locus_tag="pgaptmp_003795"
     CDS             complement(13029..14330)
                     /gene="ygiF"
                     /locus_tag="ACHKYO_RS18975"
                     /old_locus_tag="pgaptmp_003795"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HGU4430565.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            14572..15192
                     /gene="ygiM"
                     /locus_tag="ACHKYO_RS18980"
                     /old_locus_tag="pgaptmp_003796"
     CDS             14572..15192
                     /gene="ygiM"
                     /locus_tag="ACHKYO_RS18980"
                     /old_locus_tag="pgaptmp_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HGU4430566.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            15256..16494
                     /gene="cca"
                     /locus_tag="ACHKYO_RS18985"
                     /old_locus_tag="pgaptmp_003797"
     CDS             15256..16494
                     /gene="cca"
                     /locus_tag="ACHKYO_RS18985"
                     /old_locus_tag="pgaptmp_003797"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HGU4430567.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
BASE COUNT         4380 a         4171 c         3893 g         4102 t
ORIGIN      
        1 ataaactaat ttcataaaac ccgaaaagta aaatataata tttgaatgat tagcaatcct
       61 taaataacag ttactaatca ttcattttct acttatattt atataaagga atattatgtc
      121 tgcttttaaa aagtcacttc ttgttgctgg cgtagcaatg attctttcca ataacgtatt
      181 tgctgacgaa ggtcacggca ttgttaaatt taaaggtgaa gttatctctg ctccatgttc
      241 aattaaacca ggtgacgaag acttaactgt aaatctgggt gaagtagcag ataccgttct
      301 gaaaagcgac cagaaatctt tggctgagcc tttcactatt cacctgcaag actgcatgtt
      361 gagccagggt ggaaccactt attcaaaagc gaaggttact ttcaccacag cgaatactat
      421 gacgggtcag actgacctgc tgaaaaacac caaagaaact gaaatcggtg gtgccacagg
      481 cgttggcgta cgtattctgg atagccagag cggcgaagtg actttaggta ctccggtagt
      541 aattacattc aacaatacta acagctatca ggaactgaac ttcaaagctc gtatggaatc
      601 cccaagcaaa gatgctaccc cgggcaacgt ttacgctcag gctgactata agattgctta
      661 cgagtaatca cattaggccg ggttcgcccg gccacatcat taacggatta atgataagtg
      721 gaacagatgt ataaaaaatt aaaattaacc acaataagcg aattgattaa aaatatttat
      781 tgttcattat ccgttctcat cattggttgt gcgtcagctt atgccgttga attcaacaaa
      841 gatttaatcg aagccgaaga tcgtgaaaac gttaaccttt cccaatttga aactgatggc
      901 caattacccg taggtaaata ctcattaaac gctcttatta ataataaaag gacgccaatc
      961 catctggacc tgcaatgggt attaattgat aaccaaactg cagtttgtct gactccagag
     1021 caattaacat tattaggctt tactgatgag attattgaag tggctcagca aaacctgatc
     1081 gatggttgtt accctatcga aaaagaaaaa caaattacaa cttatctcga taaagggaaa
     1141 atgcaattat ccatatctgc acctcaggca tggttaaaat acaaagatgc aaactggacg
     1201 cctcctgaac tttgggatca tggtattgct ggggcatttc ttgactacaa tttatatgcc
     1261 tctcattatg caccacatca gggcgataat tcacaaaata taagttccta tgggcaggct
     1321 ggggttaatc ttggggcctg gcgcctgcgt actgattacc agtacgatca atcatttaac
     1381 aatggcaaaa gccaggcgaa caacctggat tttccgcgta tttatttgtt tcgcccaatc
     1441 ccagcaatta atgcaaaact aactataggt caatacgata ctgaatcctc tattttcgac
     1501 tctttccatt tttctggcgt ttcgttgaaa agcgatgaga atatgttgcc gcctgatcta
     1561 cgtggttacg caccgcaaat cacgggtgtt gcacaaacga atgcaaaggt cactgtctca
     1621 cagaacaacc gtattattta tcaagaaaat gttcctcctg gcccatttgc tattactaat
     1681 ttattcaata cattacaggg gcaactggac gtcaaggttg aagaagagga cgggcaagtc
     1741 acgcaatggc aagttgcatc taatagtatt ccttatctga cgcgtaaagg gcagattcgc
     1801 tacaccactg ctatgggtaa accgaccagc gttggtggtg attccttaca acaacccttc
     1861 ttctggactg gtgaattctc atggggttgg ctgaacaatg tatccctgta tggtggttca
     1921 gttttaacaa accgtgatta tcaatctctg gctgccggcg ttggctttaa ccttaactca
     1981 ttgggttcat tatcttttga tgtcacacga tctgatgctc agttgcataa tcaggataaa
     2041 gaaacgggtt atagctaccg cgctaactat tcaaaacgtt ttgaatctac cggtagccag
     2101 ctcactttcg ctggttaccg tttctctgat aaaaactttg tgacaatgaa tgaatatatc
     2161 aatgacacta accattacac gaattatcag aatgaaaaag agagttatat tgtcacgttt
     2221 aaccagtatc ttgaatcatt aaggttaaat acatacgtaa gtttggctcg taatacttac
     2281 tgggacgcca gcagtaatgt gaattattca ttttcactta gccgcgattt tgatatcggg
     2341 ccattaaaaa acgtctccac ttctctaaca tttagccgaa taaactggga agaagacaac
     2401 caggatcaac tgtacctaaa tatttcgatt ccctggggaa ccagtagaac attgagctat
     2461 ggtatgcaac gaaatcagga taacaagatt tcgcatactg cttcgtggta tgactcttcc
     2521 gatcgaaata attcctggag cgtttctgct tcaggcgaca atgatgaatt caaagatatg
     2581 aaagcgtcac tacgcgccag ttatcagcat aataccgaga acggtcgtct ctatctctcc
     2641 gggacatcac agcgagacag ttattattct ctgaatgcca gttggaatgg ttcattcacg
     2701 gcgactcgtc acggagccgc tttccatgac tatagtggta gtgctgactc gcgttttatg
     2761 atcgacgcag atggtgcaga agatattccg ttgaacaata aacgcgcggt aactaatcgg
     2821 tatggcatcg gagttattcc atcagtcagc agttacataa caacatcatt aagcgttgat
     2881 acccgaaatc ttccagaaaa tgtggatatc gaaaactcgg ttatcaccac caccttaacc
     2941 gaaggtgcta ttggctacgc caaacttgat acccgcaagg gctatcaaat catgggggtt
     3001 attcgcctgg cagatggtag tcacccacca ctggggatta gcgtaaaaga taaaaccagc
     3061 cataaagaat taggactggt tgctgatggc ggctttgtat acctcaacgg cattcaggat
     3121 gacagcaaac ttactttacg ctggggtgat aaatcctgtt ttattcaacc acccaatagc
     3181 agcaacttaa ccaccggaac ggttatttta ccgtgtatta gccaaaatta attcaggatg
     3241 aaactaatgc gctacttaaa cacaaagaat ctcattgcag caggagtatt actcgcctgc
     3301 atgagcagca ttgcctgggg tgccatcatc ccggaccgta cccgtatcat aatgaatgag
     3361 tcagataaag gggaagcatt aaaacttact aatcaaagca aaaatctacc ctatctggcg
     3421 caaacatgga tagaagatac gaaaggcaat caatcacgtg actttattgt cacggttccc
     3481 cctatggtac gtttaaatcc cagtgagcaa attcaaataa gaatgataag tcaggaaaaa
     3541 attgctcaac tgcctaatga cagagaaacg ctcttctatt ttaacgtgcg agaaataccg
     3601 ccaaaaacag ataaaaaaaa tgtgatgcaa gtcactatgc agcacgcatt gaaactattc
     3661 tggcgaccaa aagctattga attagaagat gatggggtaa tgtcttacga aaaagttgaa
     3721 atcatccgta gaaatgatgg ttcgatccgc tttaacaata agatgcctta tcacgttacg
     3781 ctgggttata tcggaacaaa cggaatcaca atgttgccac aaacgcaaag tttaatggta
     3841 accccttata gccatgcaga tacgcagttt aaaaatgttc cgtcagcttt tcaggttggt
     3901 tatatcaatg attttggtgg attaagtttt tatgaaataa actgcccgac ggttaataac
     3961 tcttgtaatg tttccgtagc caaacgagat aaataatatg cgctacttat taattgtaat
     4021 tatatttatt ataggtttta gtgcattacc tgtatgggca atggactgct atgctgaaca
     4081 tgaaggggga aataccgtag tcattggtta cgtaccaaga atttctatcc ccagtaatgg
     4141 taaaaaaggt gataagatct ggcaaagcag tgagtatttt atgaatgtct tctgtaataa
     4201 tgcattacct gccccatcac ctggagaaga atacccatca gcctggacta atataatgat
     4261 gtttttacca ggcggtcagg acttttataa tcagaactcc tatatttttg gcgtaacata
     4321 caatggagtt gattatgatt cgactgcccc taatgctatc gcagcaccag aatgcattga
     4381 tataaaagga gcgggtacct ttaataatca ctataaaaac cccgctgtct gtagtggagg
     4441 tcctgagcct caattatcag taacctttcc agctcgcgta caactctata ttaaattagc
     4501 aaaaaatgcc aatagagtta ataaaaacat ggtgttacct gatgaatata tcgctctaga
     4561 gtttaaaggt atgagtggcg caggagctat agaagtagac aaaaatttga ccttcaggat
     4621 tcgtggatta aataatattc atgtccttga ctgctttgtt aatgtcgcac tggaaccagc
     4681 tgatggcgtt gttgactttg gtaaaataaa ttcccgaaca attaaaaata ccagcgttag
     4741 tgagacgttt agtgtagtca tgacaaaaga tcctggtgcg gcctgtactg agcaattcaa
     4801 tattttaggg agctttttca ctacggatat tttgagtgat tatagccatc tggatatggg
     4861 taatggactg ctattgaaga tatttcataa agatggaaca gcaacggaat ttaaccgctt
     4921 ctcacaattt gcttcttttt catcgtctag tgcaccttcg gtcaccgcac cattcagggc
     4981 agaacttagt gcgaatccgg cagagactgt tgttgaggga ccgtttagta aagacgtcat
     5041 cctcaaaatc acctataact agcatctaat acaaacacta aaacgggcca tcaggcccgt
     5101 tatatcagtg ctctaactcc agctctcttg cctgcatcat ctgttgacaa taccaggcat
     5161 aacgtgcgca aaaccaggtt ctatgttctt catccatgtt accagtaacg gcaagaatat
     5221 cgaccgggcg accttctgcg tgcattctgg caaaactacg cgccaggaaa tcgaaattat
     5281 ttaaagaatt aagactatga tccataagca catcctccgg ttatcgcata ccttttaagg
     5341 ccatatgctt tttctgattt cagtattccc caatcagtgt agtgccttta aacataacgt
     5401 gcgtaaatat attgtccccg atcacacttt ttagtgaaaa ataaaaaacc atattaaaaa
     5461 actttctctt ccagaacttc tctaacatgt gttattatta acaaagataa atgtaagcaa
     5521 ggaaactatc ccaaagtaaa tattgcgtca ctaaatggac attggagtga catatgattt
     5581 tattcgccga ctataatacc ccttatcttt tcgctatttc ctttgtactg ttgatcggcc
     5641 ttttagaaat tttagcgctt atctgcggtc acatgctctc cggtgctctt gatgcacatc
     5701 ttgaccatta tgattccata accacaggtc atattagcca ggcacttcat tatctcaata
     5761 tcggaagatt gccagctctc gtcgttctct gcttgctggc tggttttttt ggtcttattg
     5821 gcatcctgtt gcagcatgcc tgcatcatgg tctggcaatc accactgtcg aacctgttcg
     5881 tggttcctgt cagtttactg tttacgatta ttgcggtgca ttataccggt aaaatagttg
     5941 cgccctggat tccacgcgac catagttcag ctattacaga agaagaatat attggtagca
     6001 tggcattaat taccggtcat caagccactt caggcaatcc ctgcgaagga aaactcaccg
     6061 atcaatttgg ccaaatacat tatttactac tggagcctga agaaggaaaa tttttcacta
     6121 aaggagacaa agtgttaatt atttgccgac tttctgcaac gcgatatttg gcggaaaaca
     6181 atccctggcc acaaatattg taatttatta atcaaaagga aatttaataa tggatgatat
     6241 tgttaattct gtgccctcct ggatgtttac cgcgattatt gccgtatgca ttctgtttat
     6301 tattggaatt attttcgcca ggctctatcg tcgcgcttcg gcagagcaag cttttgttcg
     6361 tactggttta ggtgggcaaa aagtggtgat gagcggtggc gcaatcgtga tgccgatctt
     6421 tcatgaaata atccccatca atatgaatac tctgaagctg gaagtcagcc gctcaaccat
     6481 tgatagcctg attacgaaag atcgtatgcg tgtcgatgta gtagtcgctt tctttgtacg
     6541 ggtaaaaccg tctgtagaag gcatcgccac tgcggcgcag acgttggggc aacgcacgct
     6601 atcaccagaa gacttacgta tgttggttga agataaattt gtcgatgccc tccgtgcaac
     6661 agctgcacaa atgaccatgc atgagttaca ggatacccgc gagaacttcg tgcagggagt
     6721 acaaaatact gtcgcagaag acctgtcgaa aaacggtctt gagctggaaa gcgtttcact
     6781 taccaacttt aaccagacct cgaaagaaca tttcaatccg aacaatgcct ttgacgccga
     6841 aggtttaacc aaactgactc aggagacgga gcgccgtcgt cgcgaacgta acgaagttga
     6901 acaggatgta gaagttgcgg tgcgtgagaa aaaccgtgat gcactttcgc gcaagttgga
     6961 gattgaacaa caagaagcgt ttatgacgct tgagcaggag cagcaggtta aaacccgtac
     7021 tgccgaacag aatgcacgta ttgcggcttt tgaagctgga cgtcgtcgtg aagcagagca
     7081 gacgcgaatt ctggctgaac gacaaattca ggaaacggag atcgagcgcg agcaggccgt
     7141 ccgctcaaga aaggttgagg ctgaacgtga agttcgtatt aaagagatcg aacaacagca
     7201 ggtcaccgaa atcgccaacc agacgaaatc gatcgctatt gccgccaaat cggaacaaca
     7261 gtcccaggca gaagcgcgtg ctaaccttgc actcgcagaa gcagtaagtg cgcaacaaaa
     7321 cgtagaaacc actcgccaga ccgcagaagc cgatcgtgct aaacaagttg ccctaatcgc
     7381 tgccgcgcag gatgcagaaa ccaaagcggt tgaactgacc gtgcgggcga aagcagaaaa
     7441 agaagccgca gagatgcagg cggcggctat cgttgagtta gccgaagcta cacgtaaaaa
     7501 gggtctggcg gaagcagaag cacaacgtgc gctgaacgat gctatcaacg tactttctga
     7561 tgaacaaacc agccttaaat tcaaactggc cttgttgcag gcgctacctg cggtaataga
     7621 aaaatccgtt gagccgatga aatctattga cggtatcaag attattcagg tcgatggtct
     7681 gaatcgtggc agcgctgcgg gtgatgcaaa cacgggtaat gtggggggcg gaaacctggc
     7741 ggaacaagca ttatcagccg ctctctctta ccgcacacag gcaccgctga ttgactcctt
     7801 gctcaatgaa attggcgttt caggcggctc actggcggca ttgacttcac ccttaacctc
     7861 aacaactccc gtcgccgaaa acgtagaata aattttcagc tatgaacgtc cctggcacaa
     7921 aaagccgggg attttttata tctgcgttcc gctaaaaact gcaaatgatc aggtcgttgc
     7981 aacgttttgc gtgaccgctc ggggaaggca aaactgcctc tgggaaagcg ttgcgcgagg
     8041 tccggcgctc atcaacaatc ggggagtcag caaagggctg aaacgggaaa gcccctcccg
     8101 aggaaggggc cttacagaag gaaagggtta tgatgaagct cgtcatcata ctgattgtgt
     8161 tgttaatcgt aagtttcgca gcttattaac agccaatcag aggggggaga aatcctccct
     8221 cacccttgtt cctttacttt aggttgaaaa aaaatcagcg tcaagaaggt ttcattaaca
     8281 catatccctg gcccaaaaag ccggggattt tttatatctg cgttccgcta aaaggtgcaa
     8341 atgctcagga cgttgcagcg ttttgcgtga ccgctcgggg aaggcaaaac tgcttcaggg
     8401 aaagcgttgc gcggggtccg gcgctcatca acaatcgggg agtcagcaag gggctgaaac
     8461 gggaaagccc ctcccgagga aggggcctta tagaaggaaa gggttatgat gaagttcgtc
     8521 atcatactgg ttgtgttgtt actgttaagt ttcccgactt actaacaact gatcagaggg
     8581 gggagaaatc ctcccttacc cttgttcctt tactttaggt tgaaaaaaca acagcgtcaa
     8641 taggcctgcc tgctacgaag cgagatctgt gaaccgcttt ccggttagcc ttttttatcc
     8701 tgttggatct tcttgatgat gttagtggtc gagcaaccgt cttcgaagtt gagcaccagc
     8761 acttcgccgc cgttggccca gacttcttta ctcccggcaa tttcttctgg tttatagtcg
     8821 ccgcccttca ccagtaaatc tggcaggatc ccggcgatca agcgctgcgg cgtgtcttct
     8881 tcaaacgaca ccacccagtc gaccgcttcc agtgcgccca gcacaatcat acgctgttcg
     8941 agcgggttaa cggggcggga atcccctttc agccgtttgg tggaggcatc gctgttcaca
     9001 gcgacaatca gacggtcacc cagtttgcga gcatttgcca gataggagac gtgcccggcg
     9061 tgcaggatgt caaagacacc gttggtcatt accacctttt cgccgcgttt acgcgctgcc
     9121 gctacagcaa gcttcagttc ctcttcggtc atcacaccaa agccggtatc tgcgcgccca
     9181 cgcactgcat tttccagctc gatcggcgaa accgtggagg ttcccagttt gccgaccacc
     9241 acgccagccg ccgcattggc aaagaagcag gcttcttcca gcgaattacc cgctgccagc
     9301 gttgccgcca ggacgccaat caccgtgtcg cccgcaccgg taacgtcata cacttcctgc
     9361 gcctgggttg gcatatgcag cggcgcttta cccggttgca gcagcgacat cccctgttcg
     9421 gaacgggtca ctaacagagc cgagagttcg taatcggcaa tcagtttcat gccgcgctca
     9481 acaatctctt cttcggtctt acatttaccg acaacagctt caaattccga gagattcggc
     9541 gttaacagcg tagcgccgcg gtagcgctca aaatcggtac cttttggatc aatcagcacc
     9601 ggaacgcccg ctttacgcgc cagttggatc atctgctgta cgcttgccag cgcacctttg
     9661 gcgtagtcag aaagcaccag cgcgccaatc gaactcagcg cctgattaat ccgctcgtgc
     9721 agcggctgcg gatcaacacc ttcgaaacct tcttcaaaat ccaggcggat cagctgttgg
     9781 ttgcgggaaa gtacccgtaa tttggtgata gtcggatgcg tcggtacaga aacgaagtcg
     9841 catttgacgt tgacgtcggc cagagattta ctcagcgcac gcgccgcatc gtcaatgccc
     9901 gtcaacccga ccaggcgtgc attagcaccg agagaagcga tattcatcgc cacgttagcc
     9961 gcgccgcccg gacgttcttc gatggtattg actttaacca cgggcaccgg cgcttccggc
    10021 gagatacggc tggtgggacc gtaccagtaa cgatccagca tcacatcacc aaccaccatc
    10081 actcctgcac gttcaaactc tggcagcgtt actttcattc ctgtctcctg agagattcaa
    10141 aatttgcgcg cgataatacc atacttcatt cttccaccag ccacttctgc cagcttgccc
    10201 gcaccagatc acgctctgcg gtgaagcaat cctccgacac atggcccggc aattcctgta
    10261 atgccagatg atgaagttca tcgcgcagcg tcgtgtaagc acgggtcagc gccatcgctt
    10321 cctgctcttc cataatgtcg ttttgcgcca gcagctccag aatacgcacg ttgtctgacc
    10381 agcgcgttaa cttcggtttt tcatgagcgt agcgcaacac cagatattgg gtaataaatt
    10441 cgatatcggt aattccgcct tcatcggctt tgatatcaaa gcgattacga tgtttattgc
    10501 cgagatgagc gcgcattttc tcgcgcattt cccgcacttc cgtttgcaga gttttacctt
    10561 cacgcggcag cgtcataatc tcgcgacgca ctgcgtcaaa gtgcgcggtg agctgcggat
    10621 cgccgtacac tacacgcgca cgcaccagcg cctgatgctc ccacgtccag gcctcgtttt
    10681 tctgataatc agcaaatgct tctgcggatg tcaccagcat tcccgccgcc ccggacggac
    10741 gcagtcgtgc atccacttca tacaaaatgc cggaagaggt tcgcgtactg aacagatgca
    10801 taatgcgttg cgccagacgc agataaaact gtcgcccgtc gatttcccgc tcgccgtcag
    10861 tcatcgcatc cattgggcaa tcatggagga agatcaggtc aagatcggaa ctgtagccta
    10921 actcccagcc gcccagcttg ccgtagccga ccaccgcaaa accacggcct tcgcgatcgt
    10981 tgaggtgatt tggcttaccg tagcgggcca ccatttgaac ccacgcctgc tgaacgacgg
    11041 catctatcat agcctctgcc agccaggtta agtgatcgct cactttcatc accggtagcg
    11101 taccagcgat atccgctgcg gcgatgcgta acagctgcgc ctgtttgaac tgacgcagcg
    11161 cctcaagctg ttgctcttcg tcatcttccg gcacgcgcag caaatactgg cgcaactcat
    11221 cgcggtaggc atcggtcgcc gtcggctggt aaagagtgtt tggatcgagc aattcatcca
    11281 gcaataatgg ataacgcgcc agctggctgg caatcatcgg cgatgcggca cacagagaaa
    11341 tcaaatgttt aagcgccgca cggaattcac tgagcaattc taaataggtg gtgcgggtaa
    11401 caatccccac cagcaaggcg gtaatgcgcg acagcgtaac ggcagcgtct tcacgcgcac
    11461 agacatcact tagcagatgt ggcatcagat ggtcgagcac ctgacgtcct cgcggcccga
    11521 tagtacgctt atccagctct ttgcggaaat cagcaatcag cgttaggacc tgtttgcgat
    11581 catcctcgct aagatgcgcc agcactggcg tagtgtcatc ttcctgcaac gcatcctgcc
    11641 acagctcacg ccactgttcc gacagcgact cttcctgagt ttcactttcg tcgtcgccaa
    11701 tcaattcatt aaacacccgg cgcacattgg tcatatgtgc ggtcagcgcc ccggtcagtt
    11761 gcggccagtc agcaaagtcc atcgcccacg ccagccgcgc acggttaagc tcatcagaag
    11821 gaagcgtttg ggtttgttcg tcgttaatgc tttgcagcag gttttccaga cgccgcagga
    11881 acagatatgc cactcgcaat tgttcagcat cgttttcaga aagcagatgc agcgcggcaa
    11941 tggcgctgag cgttggcagt aaagagcgcg actgcagcga cggttcgcgc ccaccgcgaa
    12001 tgagctggaa cacctgaacg ataaattcaa tttcgcgaat gccgcctgcg ccgagtttga
    12061 tattgtcggt caaaccacgt cgacgcactt cacgggcaat catccctttc atgttgcgca
    12121 gcgactgaat cacgctgaaa tcgatgtaac gacggaaaac aaacgggcgc agcatcgcac
    12181 gcaactcgtt agcatagacg ccttcgctat cgcccataat ccgcgccttg accatcgcgt
    12241 aacgctccca gtcgcgcccc tgctcctggt aataatcctc caacgcggca aagctcagca
    12301 ccagcgggcc actttcgcca aacggacgca gccgcatatc cacgcgatag acgaagccat
    12361 cctgcgttgg ttgatccaac actttaatca gccgctgccc catgcgggta aaaaactgcg
    12421 cgttatccag ttcccggcgt ccgccctgcg tacaaccatg ttccggccag gcaaaaatca
    12481 gatcgatatc tgaggagaaa ttcagttccc caccgcccag cttacccatg cctaaaatca
    12541 gcagcggttg cgcttcgccc tgcgcattgc acggcgttcc ccattcgcgg cagcaagcgt
    12601 catacagcca gtcacgcgcg gcgacaatca gcgtctccgc cagatggctg agctgctgca
    12661 atatgctctc ttcagtaacc aaagccagcg tttgcgccca ggcgatgcgc accataatgc
    12721 gccgccggaa tagccgcagc tcgcgcatta acccggcttc gtcactcaca ttgctgagcg
    12781 cctcctgcaa ccatgccgcg taatgctgcc attcatcagc ctgcggcgat tggctttcca
    12841 gttccgtcag ccactctgga tgcgcaatca cgctgtcctg cacaaaatca ctgaaagtaa
    12901 gtactgactt cgcctgtgcg ctaagtgatt tctcggctaa aggctctggc agccgctcaa
    12961 caacggtctg ccagtactgc tgtaacggtg aagagagcgg cttcataagc gattttatcc
    13021 ttgcctgatt aacgttttcc gctgtgcaac cagaacggtt cctgattgtt tgcctcatta
    13081 cggaaatgtt caatttcaat gcgttgcccg gtcgcaatag cgtgacgcag cccctgccag
    13141 ttctccagcc aggcttgcgc gacgacagga tcatagtaac ccgccagcaa cagtattgag
    13201 tcaatatcac gcgtcagacg tggcaactgg tcacggtagc gatcgcccaa cggctggcag
    13261 aaaacgcttt tcagttcagc ggcatggcgg gaaagatgga tatcggcaaa gcgtttgaag
    13321 gaatcgctca ttttactctg cgctttggca tctaaaaacg gctgccaggc tttgcttacc
    13381 aaccattcgg tcaacgccag ctttgccatt gccgtttcgg tagagtagac cgccgtcacg
    13441 gcagaaaccg cagaagcgat ggtcgcttcg cattgagtca gcaaatcacg taagtgagtg
    13501 ctcgctttac gcggcacaat gccaccgaac agcatcagtg catggcgaac aaggccaatg
    13561 gctgccagca cctgctcttt cgccgcatcg ttgccgcgta cccacagttc ttcatgatac
    13621 tgccattgcg ctaacgccag ctcgagcgcc gcttccagcc cctgttccac atcggctttt
    13681 gccgcaacat gcaaaatggt ggtcggtttg atttcacgcg ccggattgcc ctgcgccaga
    13741 tgatagccac gcgccgcttt gctcaggctg ccctggcgta atccggtttg cgataccagt
    13801 tggttcgcca gtttcagcac cgcgcgcgtg tcgccgctaa gcagttccag ttccagctca
    13861 cagataggtt cagcaaattc ccccgctttc acctccccct gatcgagagc gatttcaatt
    13921 cggctgtcat cgaccgccac cagccatttt tcgcgataaa aatcggtgct gaacagcggt
    13981 tgtacgcggg aagcgagatc ggcgggcaat tcgccgttcg gccagacttc cgtcggtaac
    14041 tgcgccaggt cgagcgtcgg ttcgctcaac gccacgttat attccgggcg ctggtgtaag
    14101 ccgcctgtca ctcttcctgc aactttcatg gtcatctcat agcgaccgtt ttcgccacga
    14161 atacgtaagc ccatatcgtg cccacgcagc cagttatccg gcgtttcgta gtaaatattc
    14221 agtaactgca cggggtcatg gtgctcgccg cccagcgtat tgagatggtc acgcaacgcc
    14281 tcaacggcac tgtgattaac aataaacttt aattcgattt cctgagccat ggcctttact
    14341 tatcggttat gtcacataag tgacgatgaa cggcgaactt aatacgatct tttttgtcag
    14401 tagatagtat tttgcgccaa attgccatgc aacgggcaat ttgacgggcg taaaagtttg
    14461 aagcagtggc aagacctgac acagaggatt ccgattgatg tcgaatgctt tgcgtagcga
    14521 cactgatgcc actactatcg ttccactttc catgacaata acgacagcct gatgccaaaa
    14581 ttacgcctga tcggattaac tttactcgca cttagcgcca ctgccgtctc acacgctgaa
    14641 gaaacgcgct atgtttctga tgaactgaat acctgggtcc gtagcggtcc gggagatcat
    14701 tatcgcctcg tgggcacggt taacgccggc gaggaagtga ccttattaca aactgacgcc
    14761 aacaccaatt atgcccaggt gaaagacagc tctggccgta ccgcctggat cccgttgaaa
    14821 caacttagca ctgagccaag cctgcgctcc cgtgtgccag atctggaaaa tcaggtcaaa
    14881 accctgaccg ataaactcac caatatcgat aacacctgga atcagcgcac cgcagaaatg
    14941 cagcaaaaag tggcgcagag cgacagcgtg atcaacggat taaaagaaga aaatcagaaa
    15001 ttgaaaaacg agctgattgt cgcgcagaaa aaggtcgatg ccgccagcgt acagttggat
    15061 gacaaacagc gcaccatcat catgcaatgg tttatgtatg gcggtggcgt gctggggctt
    15121 ggcttgctgc tcggtctggt actgccacac ctgatcccaa cgcgcaaacg caaagatcgc
    15181 tggatgaact aaatcgcctt cttcgttgca ctgtcttatt attaggaatt gtgtatttga
    15241 cgaagggatg atggcgtgaa gatttatctg gtcggtggtg ctgttcggga tgcattgtta
    15301 gggctaccgg tcaaagacag agattgggtg gtggtcggca gtacgccaca ggagatgctc
    15361 gacgcgggct accagcaggt aggccgcgat tttcctgtgt ttctgcatcc gcaaacgcat
    15421 gaagagtatg cgctggcacg taccgaacgg aaatccggtt ccggttacac cggttttact
    15481 tgctatgccg caccggatgt cacgctggaa gatgatctta agcgtcgcga tctgaccatt
    15541 aatgcgctgg cccaggacga taacggcgag attatcgacc cgtacaacgg tctgggcgat
    15601 ctgcaaaatc gtctgttgcg ccatgtttcc cccgcttttg gcgaagatcc gttacgcgtt
    15661 ctgcgcgtgg cgcgttttgc tgcgcgttat gcccacctcg gttttcgtat tgccgatgaa
    15721 actctgacgc tgatgcgcga gatgacccat gcgggtgaac tggaacacct gacgcctgaa
    15781 cgggtatgga aagagacgga aagcgccctc accacccgca atccacaggt gttcttccag
    15841 gtactgcgcg attgtggcgc actgcgcgtt ttattcccgg aaattgacgc actgtttggc
    15901 gttccggccc ctgccaggtg gcatccggaa atcgacacgg gtattcatac cttaatgacg
    15961 ctctcaatgg cggcgatgct gagtccgcag gtcgatgtcc gtttcgcaac tttatgccac
    16021 gatctcggta aaggactgac gccgccagaa ctttggccgc gtcatcatgg tcacggtccg
    16081 gcgggtgtga aattagtgga acaactctgc cagcgtctgc gcgtgccaaa tgaaattcgc
    16141 gatttagcca gactggtggc tgagtttcac gatctcatcc acaccttccc aatgctgaac
    16201 ccaaaaacta tcgtcaaatt gtttgattcc atcgatgcct ggcgtaaacc gcagcgtgtc
    16261 gagcaactgg cgctgaccag cgaggctgac gtgcgcggca gaaccggttt tgaatcagcg
    16321 gactacccgc aaggccgttg gttgcgcgaa gcctgggaag tggcgcagtc agtgccgaca
    16381 aaagcggtcg ttgaagcggg atttaaaggt gtggagattc gcgaggagct gacccgacgg
    16441 cggattgcgg cggtagccag ttggaaggaa caacgttgcc caaagcctga ctgagtataa
    16501 aactaaacgt taacacgaac catcccctcg ccccttcggg gagagg
//
LOCUS       DAZGAN020000106        16399 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGAN020000106 DAZGAN020000000
VERSION     DAZGAN020000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.106"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..439
                     /locus_tag="ACHKYO_RS18990"
                     /old_locus_tag="pgaptmp_003798"
     CDS             <1..439
                     /locus_tag="ACHKYO_RS18990"
                     /old_locus_tag="pgaptmp_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU4430568.1"
                     /translation="SAKEQADKLKARRRDLQPRSYWIKQAQQAVNAYIRERDRHLPCV
                     SCGTFDSAQWDAGHYRTTVAAPQLRFDERNIHKQCVVCNQYKSGNLVPYRVELINRIG
                     QEAVDEIESNHSRHRWTVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            436..630
                     /locus_tag="ACHKYO_RS18995"
                     /old_locus_tag="pgaptmp_003799"
     CDS             436..630
                     /locus_tag="ACHKYO_RS18995"
                     /old_locus_tag="pgaptmp_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HGU4430569.1"
                     /translation="MTFTVKTIPDMLVEAYGNQTEVARILNCNRATVRKYIGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            623..1057
                     /locus_tag="ACHKYO_RS19000"
                     /old_locus_tag="pgaptmp_003800"
     CDS             623..1057
                     /locus_tag="ACHKYO_RS19000"
                     /old_locus_tag="pgaptmp_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4430570.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKRLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            1564..2511
                     /gene="stxA1a"
                     /locus_tag="ACHKYO_RS19005"
                     /old_locus_tag="pgaptmp_003801"
     CDS             1564..2511
                     /gene="stxA1a"
                     /locus_tag="ACHKYO_RS19005"
                     /old_locus_tag="pgaptmp_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HGU4430571.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRGRGITHNKILWDSSTLGAILMRRTISS"
     gene            2521..2790
                     /gene="stxB1a"
                     /locus_tag="ACHKYO_RS19010"
                     /old_locus_tag="pgaptmp_003802"
     CDS             2521..2790
                     /gene="stxB1a"
                     /locus_tag="ACHKYO_RS19010"
                     /old_locus_tag="pgaptmp_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HGU4430572.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            3295..5235
                     /locus_tag="ACHKYO_RS19015"
                     /old_locus_tag="pgaptmp_003803"
     CDS             3295..5235
                     /locus_tag="ACHKYO_RS19015"
                     /old_locus_tag="pgaptmp_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4430573.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGELPSLAESGNHPHVSAKPEAAPEYYYVIALAGQSNSMSYGEGLPLPET
                     YDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYG
                     TVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVD
                     KPLYKDLIGRTKAALKKSPKNVLFAVVWMQGEFDFGGMPVNHAAQFGALVDKFRADLA
                     DMAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDEN
                     GVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSTWARRGIISDRLATAI
                     LRHAGRVALNAGASSTVSEVRPSSPSGAEVTGVTTLLSYLASESEGSLKVQGWSASGG
                     RAEVVSDAEGTGGKAVKVTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKASGALAA
                     NQYVMAFYWPVSSLPQGVVLTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLG
                     SFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFSADKLHVTDITK
                     SATYPVLIDSIAVEVNNADAAA"
     gene            5371..5550
                     /locus_tag="ACHKYO_RS19020"
                     /old_locus_tag="pgaptmp_003804"
     CDS             5371..5550
                     /locus_tag="ACHKYO_RS19020"
                     /old_locus_tag="pgaptmp_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4430574.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLV"
     gene            5591..5836
                     /locus_tag="ACHKYO_RS19025"
                     /old_locus_tag="pgaptmp_003805"
     CDS             5591..5836
                     /locus_tag="ACHKYO_RS19025"
                     /old_locus_tag="pgaptmp_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4430575.1"
                     /translation="MSEITSLVTAEAVKDVLRSEEVLSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGGGSASDSGDPTPDSDMMM"
     gene            5914..6129
                     /locus_tag="ACHKYO_RS19030"
                     /old_locus_tag="pgaptmp_003806"
     CDS             5914..6129
                     /locus_tag="ACHKYO_RS19030"
                     /old_locus_tag="pgaptmp_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4430576.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            6134..6667
                     /locus_tag="ACHKYO_RS19035"
                     /old_locus_tag="pgaptmp_003807"
     CDS             6134..6667
                     /locus_tag="ACHKYO_RS19035"
                     /old_locus_tag="pgaptmp_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4430577.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            6942..7514
                     /locus_tag="ACHKYO_RS19040"
                     /old_locus_tag="pgaptmp_003808"
     CDS             6942..7514
                     /locus_tag="ACHKYO_RS19040"
                     /old_locus_tag="pgaptmp_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGU4430578.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPIVEGVGLDWKTQYRKL
                     KQRFNSCMVEMTIQLPGDTQRRPVICLALRKLAGWLQTISPNKVKLKIRDKVIQYQEE
                     CDDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHV
                     SKIRTLINEANLLIDFVLADTGKGKITKAD"
     gene            7514..7663
                     /locus_tag="ACHKYO_RS19045"
                     /old_locus_tag="pgaptmp_003809"
     CDS             7514..7663
                     /locus_tag="ACHKYO_RS19045"
                     /old_locus_tag="pgaptmp_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430579.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVMLVAIFFSTAWRVLTL
                     SGLVG"
     gene            7671..8108
                     /locus_tag="ACHKYO_RS19050"
                     /old_locus_tag="pgaptmp_003810"
     CDS             7671..8108
                     /locus_tag="ACHKYO_RS19050"
                     /old_locus_tag="pgaptmp_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4430580.1"
                     /translation="MNRVLCVVIIVLAVGCGALSLGLNHYRDNSIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            8307..8804
                     /locus_tag="ACHKYO_RS19055"
                     /old_locus_tag="pgaptmp_003811"
     CDS             8307..8804
                     /locus_tag="ACHKYO_RS19055"
                     /old_locus_tag="pgaptmp_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU4430581.1"
                     /translation="MKAITLFNTPIRVDESGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            8801..9058
                     /locus_tag="ACHKYO_RS19060"
                     /old_locus_tag="pgaptmp_003812"
     CDS             8801..9058
                     /locus_tag="ACHKYO_RS19060"
                     /old_locus_tag="pgaptmp_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430582.1"
                     /translation="MSDRVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            9284..9658
                     /locus_tag="ACHKYO_RS19065"
                     /old_locus_tag="pgaptmp_003813"
     CDS             9284..9658
                     /locus_tag="ACHKYO_RS19065"
                     /old_locus_tag="pgaptmp_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HGU4430583.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPEKIEGLICSEHFFTAAQGDHQA
                     KEDDLIYNPEPYVEATPDALHLLTFCVLVLKNGFTVTGESACASPENFDAEIGRKIAR
                     QNAVNKIWMLEGYLLKQKLSEQ"
     gene            10067..10873
                     /locus_tag="ACHKYO_RS19070"
                     /old_locus_tag="pgaptmp_003814"
     CDS             10067..10873
                     /locus_tag="ACHKYO_RS19070"
                     /old_locus_tag="pgaptmp_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4430584.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPCNDAGSGDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVVTPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            10854..12560
                     /locus_tag="ACHKYO_RS19075"
                     /old_locus_tag="pgaptmp_003815"
     CDS             10854..12560
                     /locus_tag="ACHKYO_RS19075"
                     /old_locus_tag="pgaptmp_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4430585.1"
                     /translation="MTFRKNEPRCDEPSEMTEAEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFEHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLSLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYINKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPL
                     IVYDIEPVTGRKTKAQSLRDGNKAEQHRTLMNYLLVWELPDPDEEYVCGADIAEGLEH
                     GDRSSLDIIRCSNGEQVAHWFGHLDAELFAHLIAQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            12560..14704
                     /locus_tag="ACHKYO_RS19080"
                     /old_locus_tag="pgaptmp_003816"
     CDS             12560..14704
                     /locus_tag="ACHKYO_RS19080"
                     /old_locus_tag="pgaptmp_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGU4430586.1"
                     /translation="MKNETNTMATKNDNGATPRFSQRQLQALCSDIDSQPKWRDAANK
                     ACAYYDGDQLPPEVLQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLVVMSDEPDD
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRQQNEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            14862..15869
                     /locus_tag="ACHKYO_RS19085"
                     /old_locus_tag="pgaptmp_003817"
     CDS             14862..15869
                     /locus_tag="ACHKYO_RS19085"
                     /old_locus_tag="pgaptmp_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430587.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDSDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGIGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            15893..>16399
                     /locus_tag="ACHKYO_RS19090"
                     /old_locus_tag="pgaptmp_003818"
     CDS             15893..>16399
                     /locus_tag="ACHKYO_RS19090"
                     /old_locus_tag="pgaptmp_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HGU4430588.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTA"
BASE COUNT         4385 a         3642 c         4532 g         3840 t
ORIGIN      
        1 ttcagcaaaa gagcaggcag acaagctgaa agcgcgcagg agggacttac agccccgcag
       61 ttactggatt aagcaggcac aacaggctgt gaatgcttat atcagggagc gggaccgtca
      121 tttgccgtgt gtttcatgcg ggacgttcga ttcagcccag tgggatgcag gccattaccg
      181 tacaacagtt gcggcacctc agctcagatt tgatgaacgc aatatccata agcaatgcgt
      241 ggtgtgtaac cagtacaaaa gcggaaatct cgttccgtat cgtgtcgaac tgattaaccg
      301 catcgggcag gaagcagtag acgaaatcga atcaaaccat agtcgccacc gctggactgt
      361 cgaagagtgc aaggcgatca aggcagagta tcaacagaaa cttaaagacc tgcgaaacag
      421 cagaagtgag gccgcatgac gttcaccgta aaaaccattc ctgacatgct tgttgaggca
      481 tatggaaatc agaccgaagt ggcccgaata ctgaactgca atcgtgccac agtcagaaaa
      541 tacattggcg ataaagaagg gaaaagacac gctatcgtca acggtgttct tatggttcac
      601 cgcggatggg gtaaagatac tgatgcgtga tatccggcag gttcttgagc gctggggggc
      661 atgggcggca aataaccatg aggatgttac atggtcgccc attgctgccg gatttaagag
      721 gctgatcccc gaaaaagtaa aatcacgtcc acagtgttgt gacgatgacg cgatgattat
      781 atgcgggtgt atggctcgcc ttaacaggaa caacagcgat ctgcatgact tgctggttga
      841 ttattacgtg ttgggggaga cgttcatggc gctggcacgg aaacatgggt gctctgacac
      901 ctgtataggt aaacgccttc acaaagcgga ggggattgtt gaaggcatgc tgatgatgct
      961 gggagtgagg cttgagatgg atcggtatgt tgagcgtgaa ttgccgggag ggagaacctc
     1021 tgtattttat cagcgaaaaa atagtttacg atcgtaaaaa tctgcatatc atgataagag
     1081 tggttacatt gccacgctgc ttaacccgcc gatgcgcggg tttttttgta cccagaatcc
     1141 tgtgagctat acggaaagta cacagaaagg aaggtgcgac cacaattaat aacaaaatct
     1201 taaaaatcgc acatggcact attagttttc taaatattgt gtattttttg tattgcagga
     1261 tgaccctgta acgaagtttg cgtaacagca ttttgctcta cgagtttgcc agcctccccc
     1321 agtggctggc ttttttatgt ccgtaacatc ctgtgtatca ataaatgttg ttgtctacgt
     1381 acgtcaagta gtcgcatgag atctgaccag atatgttaag gttgcagctc tctttgaata
     1441 taattatcat tttcattacg ttattgttac gtttatccgg tgcgccgtaa aacgccgtcc
     1501 ttcagggggt ggaggatgtc aagaatatag ttatcgtatg gtgctcaagg agtattgtgt
     1561 aatatgaaaa taattatttt tagagtgcta acttttttct ttgttatctt ttcagttaat
     1621 gtggttgcga aggaatttac cttagacttc tcgactgcaa agacgtatgt agattcgctg
     1681 aatgtcattc gctctgcaat aggtactcca ttacagacta tttcatcagg aggtacgtct
     1741 ttactgatga ttgatagtgg cacaggggat aatttgtttg cagttgatgt cagagggata
     1801 gatccagagg aagggcggtt taataatcta cggcttattg ttgaacgaaa taatttatat
     1861 gtgacaggat ttgttaacag gacaaataat gttttttatc gctttgctga tttttcacat
     1921 gttacctttc caggtacaac agcggttaca ttgtctggtg acagtagcta taccacgtta
     1981 cagcgtgttg cagggatcag tcgtacgggg atgcagataa atcgccattc gttgactact
     2041 tcttatctgg atttaatgtc gcatagtgga acctcactga cgcagtctgt ggcaagagcg
     2101 atgttacggt ttgttactgt tacagctgaa gctttacgtt ttcggcaaat acagagggga
     2161 tttcgtacaa cactggatga tctcagtggg cgttcttatg taatgactgc tgaagatgtt
     2221 gatcttacat tgaactgggg aaggttgagt agtgtcctgc ctgactatca tggacaagac
     2281 tctgttcgtg taggaagaat ttcttttgga agcattaatg caattctggg aagcgtggca
     2341 ttaatactga attgtcatca tcatgcatcg cgagttgcca gaatggcatc tgatgagttt
     2401 ccttctatgt gtccggcaga tggaagaggc cgtgggatta cgcacaataa aatattgtgg
     2461 gattcatcca ctctgggggc aattctgatg cgcagaacta ttagcagttg agggggtaaa
     2521 atgaaaaaaa cattattaat agctgcatcg ctttcatttt tttcagcaag tgcgctggcg
     2581 acgcctgatt gtgtaactgg aaaggtggag tatacaaaat ataatgatga cgataccttt
     2641 acagttaaag tgggtgataa agaattattt accaacagat ggaatcttca gtctcttctt
     2701 ctcagtgcgc aaattacggg gatgactgta accattaaaa ctaatgcctg tcataatgga
     2761 gggggattca gcgaagttat ttttcgttga ctcagaatag ctcagtgaaa atagcaggcg
     2821 gagattcata aatgttaaat acatctcaat tcagtcagtt gttgccggtc tgataataga
     2881 tgtgttagaa aatttctgca tggtgaatcc ccctgtgcgg aggggcgact ggtgaacggt
     2941 atgatctctt tgatgatcgt aagcgagaat acgcgggttt ggtggcacca ggccgaactc
     3001 accgggaggc acccggcacc atgcagtatt cagagattag gcatatatcc aggctcctca
     3061 tcgcaggagc ctttttacat gcaaaaaaaa accgctcctg ggaagagcgg ctggcaagaa
     3121 gaaacaatat gaacaattaa ttaacgatgg gaataatacc ttagagtaat caccttgcgc
     3181 aactgtaagg gtatatttca tctttgcggg ctgtttttct gtgtggcttc tgtgtttccg
     3241 gagggcagct tgtacctttt ctgactcaga acattatccc ggccgggagg attcatggca
     3301 tttaaacact acgatgtggt cagggcggca tcgccgtcag accttgctga acgaataact
     3361 caaaaactga aggaagggtg gcagccttat ggtagtgcgc tgatttcgac agctggttat
     3421 ggtgcagagt ttatccagcc agttgtgagt gagggggagc ttccctcact tgcagagtca
     3481 ggcaatcacc cgcatgtttc agcgaagcct gaagcagcgc cggaatatta ctatgtgatt
     3541 gcgcttgccg gtcagtccaa tagcatgtca tacggtgaag gtcttccgtt gccggagaca
     3601 tatgaccgtc cggacccgcg tattaagcag ctggcgcgcc gcagtacggt gacaccgggc
     3661 ggtgccgcat gcaaatataa cgacatcatt ccggcggacc attgtctgca tgatgtgcag
     3721 gacatgagcc gccttaacca tccgaaagcg gacctgtcaa aggggcagta cggaaccgtg
     3781 gggcagggac tgcatatcgc caaaaaactg ctgccgttta taccggcgaa tgcgggcatt
     3841 ctgctggttc cgtgctgtcg tggtggttca gcgttcacca ccggagctga tggcacatac
     3901 agtgacgcga gtggtgcctc ggagaattca acccgctggg gtgtggacaa gccgctgtat
     3961 aaggacctta tcggtcgaac aaaagcagca ctgaagaaga gcccgaaaaa tgtgctgttt
     4021 gccgtggtgt ggatgcaggg ggaatttgat tttggcggta tgccggtaaa tcacgcagca
     4081 cagtttggcg cgctggttga taaattccgt gcagacctgg cggatatggc aggccagtgc
     4141 gtcggtggct ctgctggtgg tgttccctgg atatgcgggg acacgacgta tttctggaag
     4201 cagaagaacg aatccacgta ccagacggtg tacggcagct ataaaaacaa aacggaaaag
     4261 aatatccatt tcgtaccgtt catgacggat gagaacgggg tgaatgtgcc gacgaacaaa
     4321 ccggaagaag acccggacat tccgggtatc gggtattacg gttcgaaatg gcgtgacagc
     4381 tcagccacct ggacgtcaca ggacagggcg agccatttca gcacctgggc acgccggggg
     4441 attatttccg accgtctggc aacggcgatt ctgcgccatg cgggaagagt ggcgctaaac
     4501 gcgggggcat catcgacagt atcagaggtg cgtccgtcat cgccttccgg tgcagaagtc
     4561 acaggcgtca caacactgct ctcttacctt gccagcgagt cagagggaag cctgaaagta
     4621 cagggatggt cagccagtgg cggcagggca gaagtggtca gcgatgcgga gggaaccgga
     4681 ggtaaggctg tgaaggtgac caaggaagcc ggtaaaagca gctgggtgct ggagtacgct
     4741 gcaggcaacg gtgcggctct gttacagaaa ggggggcaga ttcgctgccg ctttaaggct
     4801 tcgggagcgc tggctgcgaa ccagtatgtt atggcgtttt actggccggt atcgtcactg
     4861 ccacagggcg ttgtcctgac cggcgatggg gggaataacc tgctggcagc gttctacatc
     4921 cagacggatg caaaagacct gaatgtgatg taccacaatg cgaaagtggc gacaaacaac
     4981 ctgaaactgg gaagctttgg cgcatttgat aacgaatggc atacgctggc tttccgcttt
     5041 gccgggaata acagccttca ggtgacgccg gttattgatg gtcaggatgg cacaccgttc
     5101 acgctgacgc agtcaccggt cagtgcattt tcggcggata agttgcatgt gacagacatt
     5161 accaaaagtg cgacttaccc ggtactgata gacagcattg cggtggaagt gaataacgcg
     5221 gacgccgctg catgataaaa aaagccgcca gttaccggaa tggactggcg gtggtaatac
     5281 ctatggagaa aaaataaagg aacgatactt tcgtactctg tttttttaaa tgaaaacagt
     5341 tcttattgtc aacaataacg gaaagaaatt atgacatttc tgaaccagtt aatgctgtac
     5401 ttctgtacgg tggtctgtgt gctgtatctc ctttcgggtg gataccgggc catgcgtgac
     5461 ttctggcgca gacagattga caaaagggcc gctgagaaaa tcagcgccag tcagtcagcc
     5521 ggaagcaaac ccgaagagcc gctcgtttag cggcaacttt cttaatcaca cctttcgacg
     5581 agaaaatccc atgtcagaaa ttacatccct ggtcactgct gaagcagtga aggacgtcct
     5641 gcgctctgaa gaagtcctga gcgcactgaa acagaaactt cgccataacc tggaagcgcg
     5701 tcttgatgca gaagtggatg ccattctgga tgaactgctg ggcgcaccgg cagctccgga
     5761 gccggaaggc atcgcgggag gggggagtgc ttcagatagc ggtgacccca cacctgacag
     5821 cgacatgatg atgtaagcat gcgtcaggga ccatcggtgt gtgccggtgg tcttttttat
     5881 tgttgtgagc ttccggattg cgggaggcgg ggtatgtacc agatggaaaa aatcacaaca
     5941 ggtgtgtcat acaccacgtc agcggtggga acgggctact ggttcctgca gttgctggac
     6001 agggtttccc cgtctcagtg ggcggcaata ggcgtgctgg ggagtctgct gtttggtctg
     6061 ctgacgtacc tgacgaacct gtatttcaaa atcagagagg accggcgtaa ggcggcgcgg
     6121 ggagagtaag ctgatgagcc ggaaattccg ctatggttta tcagctgtcg ttctggcgct
     6181 gattgccgca ggggcttctg cgcctgaaat cctggatcag tttctggatg aaaaagaagg
     6241 taaccacacc acggcatacc gtgatggtgc ggggatctgg accatctgcc gtggagccac
     6301 ccgggtggat ggtaagcctg tgattcctgg catgaagctg tcgaaggaaa aatgcgaccg
     6361 ggttaacgct atcgaacggg ataaggcgct ggcatgggtg gagaaaaaca tcaaagtgcc
     6421 actgactgaa ccccagaaag cgggtattgc gtcattctgt ccttacaaca ttggcccggg
     6481 taagtgtttc ccgtcgacgt tttataaacg aattaatgca ggagatcgaa aaggtgcctg
     6541 cgaagcgatt cgctggtgga ttaaggacgg tggcagggac tgccgtattc gctcaaataa
     6601 ctgttatggt caggtatccc gtcgtgacca ggagagcgcg ctggcgtgct ggggaatcga
     6661 cagataagca gaatattttg ctgataaatg acgcttgctc atgcgggcgg ataacacgaa
     6721 atcctgcgaa ctggcaaaat gcaagtgaat aaagtcagga agattgtttt acgcagaggc
     6781 accgtaatgg tgtctttgtc atttctgcgc ttcgcacaag cgtaaataaa ccaaagaacc
     6841 tttcaggatg agccctggtg gataaccggc agtggtctgg ttaaccctct ttgggctggt
     6901 tattcctgtg cgcagggttc atcactaaaa ggaaataacc gatgaatatg atgaccgtgc
     6961 cgtttcacgg cgattctctt tatgtggtta accataatgg cgaaccgtat gttccaatga
     7021 aaccgatcgt tgaaggggtg gggctggact ggaaaacaca gtatcgcaag ttaaaacaac
     7081 ggtttaatag ctgcatggtc gaaatgacca ttcagctacc aggtgatacc caacgtcgcc
     7141 ctgttatctg cctggcttta cgtaaacttg ctggctggct tcagactatc agcccaaata
     7201 aggttaagct caaaatccga gacaaggtga tccagtatca ggaagagtgc gacgatgttc
     7261 tctatgaata ctggacgaag ggttttgtcg ttaatccccg tcgaatgagt gtgatggaag
     7321 aactcaatca ggcttgcgct gacatgaaac gggataaaaa cattgccagt gtgtttgcta
     7381 ccgggctgaa tgagtggaaa caggttaaag ccgcgcatgt atcaaaaatc cgcacattga
     7441 taaacgaagc gaatctgctg attgattttg tcctagctga tacaggcaaa gggaaaataa
     7501 caaaggcgga ttgatggagt ggtggctaat gatatcggat aaactcataa cgctggcgaa
     7561 gatcctctgt gtaatcgtcg gcatttcatt tttagtcatg ctggttgcca ttttcttttc
     7621 caccgcctgg agagtcctga cgttatcggg actggtgggg tgaaagagag atgaaccgtg
     7681 ttctgtgtgt ggtgattatt gtcctggcgg ttggctgtgg tgcgcttagt ctggggctga
     7741 atcattaccg tgataactcc atcgcctaca aagagcagcg cgataaagcc acatccatca
     7801 tcgcagatat gcagaagcgg caacgtgatg tagcagaact tgacgccaga tacacaaagg
     7861 agcttgctga tgctaatgcg actatcgaaa gtctccgtgc tgatgtttct gctggtcgta
     7921 agcgcctgca agtcgccgcc acctgtgcaa agtcaacgac cggagccagc agcatgggcg
     7981 atggagaaag cccaggactt acagcagatg ctgaactcaa ttattaccgt ctcagaagtg
     8041 gaatcgacaa gataaccgcg caggttaact acctgcagga atacatcagg acgcaatgcc
     8101 tgaaataatt tttttgcaaa tcacaaagtc aatttaatga gcctcgcgat gcggggcttt
     8161 tttatgtccg cagtaaacgc gcatctcacg cgcatattaa cgagagcctt tcagtaagcg
     8221 ggcctgagaa ttgccgttat aggtggcgac ctctctcggg cggcttttct gtgagacagg
     8281 ctcactttct aaaaggtaaa gacgccatga aagcaatcac gctttttaat acaccgatcc
     8341 gtgttgatga atcaggaatg atctgcctca ctgatatgtg gaaagccagt ggtaaaagtg
     8401 aatctgaatc tccgtaccac tacctgcgaa acaagcagac caaagagttc ttagccgagc
     8461 tggagaaaaa ccacgaatct gtggttttta ctgagcgcgg tgtacacggt ggaacatatg
     8521 gcgggaagtt tgttgcttat gattatgcag catggctaaa ccccggattt aaatatgcag
     8581 cctataaagt cctggatgac tacttcaccg gagaacttca gcatcgcaac agcttaagtg
     8641 cgcagctcaa tatgaagtgt catgagtttg atcagaaaaa agatatggcg agcttctgtg
     8701 ggcaaggtct ggcggcatgg cgctatacga aaccagtgtt ggtcgctgag attaactccc
     8761 tggctaacca gctgcagata acgatccccg ggcttccggg atgagtgatc gtgtcattga
     8821 atgcgcctcc agagcggggc gcgacttctc agagttcatg aaaggcgaga agggcatgat
     8881 ggaagcattg gcctcggtgg atgagtttgg cgagcagctg cgcctcaacg gctgtgtcaa
     8941 tcatcacttt gttagctaca tgatgcggaa ctcgatcatg caggcattca tggacatggc
     9001 aaaagccgag aggaaagaag agcgccggcg taagcgagcg gaagcaaaag cgaagtagcc
     9061 attacaaagc ctacctacgg gtgggcttga taatgaaacc ggaatttatt ctgggcaacc
     9121 agttacggca gtacagtgaa acaacccaag ccagtaagtg gggaaataac actggcagcc
     9181 actgaaagat gaacctccag ccttatggca aaaaagattc tttgtggtgg cagactgatg
     9241 gaaagacatc ctaatcaagc aaccactcca cagggtcata attatgaacg accagcaaat
     9301 cgaaaaagaa atcgttgaga aaggcaaaac ggcaccgcgt gtaacacctg agaaaattga
     9361 aggcctcatt tgcagtgagc attttttcac tgctgcgcag ggtgaccacc aggctaaaga
     9421 agatgacctt atttataatc cggagcctta tgtcgaagcc acccctgatg ctctgcacct
     9481 tcttactttc tgcgtattgg tactgaagaa tggcttcacc gttaccggag agagtgcctg
     9541 tgcaagtccg gaaaattttg atgcagaaat tggtcggaag attgcccggc agaatgctgt
     9601 aaacaaaatc tggatgctcg aaggttactt gctgaagcag aagctaagcg aacagtagtt
     9661 attacaaaag ccattcccta cagagtggct ttgataatgg cttataccct acacgggata
     9721 acttaattga tatccttttt aacggataaa ggcatttcag cctgacatag ccatgcgccg
     9781 tatcgttgcc gtattcctgc attaacagag accgcagccc gacagcgaga ctcctctgcg
     9841 tgagtgtgcg gggataatca aaaacgatgc acaccgggtt ttctcatttt tcacgagatg
     9901 ggagcgattt cccgcgaagc cgcctgtccg gtgcggtggt ggaagaaacc ggataaaaca
     9961 accgcattgt gcaaatatcg atcaaatatg gtgctgctgt gtgaaatctg aaaaatcaca
    10021 gcggtcatta tgcatcagtt tttaacacag gacgttagaa cgtgacatgg caaagctgga
    10081 ctggaaaaag ctggagcagg cattccgacg cgaacatgcc gaaacgggaa taacattact
    10141 ggactggtgc cggaagaaaa agattaatta caacaccgcc agaacccgta taaaaatggg
    10201 caaaatcgat catgaaattg atcataaaac cgatcatgaa atcgatcatg acatctcaga
    10261 tgaagaaccc tgcaatgacg cgggttccgg cgatgaaaaa tgcgcaaaaa actctgaaaa
    10321 aaactgcgca aattcggcag aaacgaaacg gattcgtggt tcccgacttt tacctccttc
    10381 aaacgctttt tctcagcgaa acacccacgc cgtaagacac cgtggatatg cgaagtatct
    10441 tgaggcagat aacctcatgg atgatgcgtc cgacatggtg ctgttcgatg aactggtgtt
    10501 cacccgggcc cgcgcacttt cagtaactaa ggcacttaaa gggatgttcg ccgacctgga
    10561 agaggcaact gacgtggaaa cccgtgttgc tctttacgac aaaatactca aagctgaaca
    10621 ggcccttgac cggaatattg cccgtatcga gtcaattgaa cgctcattgc tgacgctgga
    10681 cgtcctggct gagacagcac caaaacttcg tgctgaccgg gaaagaatca acgccgccag
    10741 agataaactc agagctgaaa ccgatattct gaccagccag cgtcggggcg ttgttacgcc
    10801 tgtcagtgac atcgtgtcat cgctgcatga aatgagtaat tcggggagac tggatgacat
    10861 tccggaagaa tgaaccgcga tgtgatgagc cgtcagaaat gaccgaggct gaacaacgtc
    10921 tgttcatcat gactaaactg agcaatccct ggtggcggct caatcatctc tacaaaatac
    10981 agaacgaaaa aggtgaactg gtcaccttca gaatgcgacc ggcgcagcgc cagttgtttc
    11041 gtaacatgca taataaaaac attatcctga aagcgcgcca gctgggattt tccacagcca
    11101 ttgatattta tcttctcgac caggcattat tcattccgca tctcaaatgc gggatcgtcg
    11161 ctcaggataa acaggctgcc agtgaaattt tccgcacaaa aattgctgta ccgtttgagc
    11221 atctccctga ctggctgaga gcctcattca ccatcgttga acgtcgtagc ggtgccagcg
    11281 gtggctatat cctgtttggt cacggctcga gtatccaggt ggcaacctca ttccgttcag
    11341 gtacggtgca gcgcctgcat atctcagagc acggcaaaat ttgcgcgaaa tatccggcta
    11401 aggcgaaaga actgcgaacc ggtacgctta atgccgtctc tgatgaatgc attatttttg
    11461 atgagtccac agctgaaggc gtgggtggtg atttttacga gatgagtaac cgagcacagg
    11521 agatcacagc atcaggctta tcgttgacgg cacaggatta taaattccat ttttacgcct
    11581 ggtggcagga tcctaaatac agcgcccgag tgccggaaag cgggctgaag ctgtcacggg
    11641 aaaaaatgac gtatttttct gcggttgaga aggcaatgaa catcacgctt actgatgaac
    11701 agaagcagtg gtacatcaat aaggaaactg aacagcgtga ggaaatgaag caggagtttc
    11761 cctcaacgcc acaggaggcg tttctgacgt ccggacgacg tgtgttcagt gccgaaagta
    11821 cgttgcaggc agaatcattc tgttcgccac cgctgattgt ttatgacatt gaacctgtta
    11881 caggaaggaa gactaaagcg cagtctctgc gtgacgggaa taaagccgaa cagcaccgga
    11941 cgctgatgaa ttatctgctg gtctgggagc taccggatcc agatgaagag tatgtctgcg
    12001 gagcggatat tgctgagggg cttgagcacg gggaccgctc atcgctggat atcatcagat
    12061 gcagtaatgg tgagcaggtg gcccactggt ttggtcatct tgatgcggag ctttttgctc
    12121 atctcattgc gcaggtctgt cgtatgtaca acaacgcgtt tgtggggccg gagcgtaaca
    12181 atcacggaca tgccgttatt ctgaaactca gggagctcta tccgacgcgt tatatctaca
    12241 acgaacagca ccttgatcag gcatatgatg acgatacgcc ccgccttggc tggctgacaa
    12301 cccgtcagag caaacctgtt ctgaccgaag gaatgaaaac gctcctgaat aatggaatat
    12361 cagggatccg ctggtcaggc acattatcgg aaatgaacac ctacgtttat gacgcgaaag
    12421 gctccatgaa tgcacaggaa ggctgttttg atgatcaact catgagctac atgattgccc
    12481 aggagatgcg cgccagaatg cctgtgaggg taaaacagaa aacggataaa cgcagaacca
    12541 cacactggat ggctcactga tgaaaaatga aactaacacc atggcgacga aaaacgacaa
    12601 tggagccacg ccgcgttttt ctcagcgcca gttacaggcg ctttgttctg atattgacag
    12661 ccagcctaaa tggcgtgatg ccgcaaacaa ggcctgtgcg tattacgatg gcgatcagtt
    12721 gccaccggaa gttcttcagg ttctgaaaga tcgcggtcag ccgatgacta tccataacct
    12781 catcgcgcct accgtcgatg gcgttctggg aatggaggcc aaaacacgga ctgatctggt
    12841 ggtgatgtca gacgagccag atgatgaaac tgaaaaactg gctgaagcta ttaatgctga
    12901 atttgccgat gcatgccgcc ttggcaatat gaataaagcc cgctctgatg cctatgcgga
    12961 acaaatcaag gcgggcctca gttgggtgga ggtcagacga aacagcgatc cgttcgggcc
    13021 tgaatttaag gtgtctactg tcagccggaa tgaggttttc tgggactggc tgagccggga
    13081 ggctgattta agtgactgcc gctggctgat gcgtcgccgc tggatggata ccgatgaggc
    13141 aaaagctaca ttcccgggaa tggctcaggt tatcgattat gccattgatg actggcgtgg
    13201 ttttgtcgat accacggtta ctgaaggcca gcccagtccg ttgatgagtg catgggaaga
    13261 gtatcagtca tgggatcgac agcagaacga atggcttcag cgtgaacgcc gtcgtgtgct
    13321 gcttcaggtg gtttattacc gtacattcga gcgtcttccg gtgattgaac tcagtaatgg
    13381 acgggtggtg gcctttgata aaaataatct gatgcaggcg gtagctgtgg catccgggcg
    13441 ggttcaggtg aaagtcgggc gggtaagccg tattcgtgaa gcctggtttg tcgggccaca
    13501 ctttattgtg gatcgcccct gtagtgcacc gcaggggatg tttccgctgg ttcctttctg
    13561 gggataccga aaggataaaa ccggggagcc atacgggtta atttcccgcg ccattccggc
    13621 acaggatgag gtgaattttc gtcgtatcaa gctgacctgg ctgcttcagg ccaaacgcgt
    13681 gattatggac gaggatgcca cccagttgtc agacagcgac ctgatggagc agatcgaacg
    13741 tccggatggc attattaaac tgaatccggc ccgaaaaaat cagaaaagtg tcgcagatgt
    13801 ttttcgggtt gagcaggatt ttcaggttgc cagccagcag tttcaggtca tgcaggaatc
    13861 ggaaaaactt atccaggata ccatgggagt gtattccgca tttctcgggc aggattcagg
    13921 tgcgacgtca ggcgtggcta tcagtaacct ggtggagcag ggggccacaa cccttgcgga
    13981 aatcaacgat aactaccagt ttgcctgcca gcaggtggga agactgttgc tggcttatct
    14041 tctcgatgac ctgaaaaaac gccgtaatca tgcagtggtg attaatcgcg atgatcgcca
    14101 gcgtcgccag accattgtcc tcaatgctga aggtgataat ggtgaactga ccaatgatat
    14161 ttcaaggtta aatacacata ttgcgctggc gcctgttcag cagacaccgg cgtttaaggc
    14221 acagcttgca cagagaatgt cagaggttat tcaggggctg ccgcctcagg tgcaggctgt
    14281 tgtgctcgac ctgtgggtta atcttctgga tgtgccgcag aaacaggagt ttgttgagcg
    14341 tattcgtgct gcgctgggga cgccaaaatc accggatgaa atgacgccgg aagaacagga
    14401 agtagcggca caacaacagg cacttcagca acaacaggca gaactccaga tgcgcgagat
    14461 ggctggcaga gtggcaaaac tggaagctga cgccgccagg gcacatgcag ctgcacaacg
    14521 ggataatgcc agtgcacagc gggaagtcgc cctgacacag gggcagcgtt atgtggatgc
    14581 gcttaaccag gcacatacgg cagaaatcat taccggcgta cagaatatgg aacaggagca
    14641 ggacgttctt cagcaacaga tgctgtatac gttacaacag cggatgaatg aaatgtcgct
    14701 ctgaaaactc tggcttcaac tgaaccccgt catcgtacgg ggttttttgt ttccggaggt
    14761 aagcgttccg ggagcggtgc gcttattcgc gggggcagcg ataagcctta tttactcaac
    14821 cattcggatc tgtccgataa acagaccatg cggagttatt tatggatttt gaatttacgg
    14881 gtgaagaaac cccggaacaa ctggaaaaaa tgctggaagg acttggggat gtggatattg
    14941 acagtcacgc acaggacgtc gtgacggaag ataccacgga aaaacatgcg gatgaggaag
    15001 cacagactca gacgggcgat aacaatgtgg caccgacgcc ggatgccagt gtggagcaga
    15061 cgcaggacgt gaaggagccg gaagcgaagg gggtgctcac ccgcgacggt aaacacgtca
    15121 ttccctatga agtccttgag gctgaacgtt ccggtaagca acgggccgaa caggaagccg
    15181 cacttcttcg tgggcagata gctgaagaaa aacgcagggt ggaactactg acgtctcaga
    15241 tccaccaggc cggtatgaag cccacaccgt taccggaaaa cgaaaaaatt tctgatgagc
    15301 agattgcccg tatcagggag atgtatccgg aaattggtga cgcggtggct tcgctcatcc
    15361 gtaaaaataa ctatctccag tcccgtgttc agcaatcaac acagcaggca gaaggtaatg
    15421 gtggtgagga tttatcaccg gttcttgatg cgatgaatgc cgtgccggtg ctgaaaacgt
    15481 ggcaggagtc cgattcggat cgcttctcgg ttgctgtatc catcgacggg aagctccaga
    15541 atgaccccgc atggaaagac aaaacgctca ctgaacgttt cgctgaagtg gcccgtcgta
    15601 cgcaggttgc tttcggtgaa gtcagtgagt cgtctgctga caaccaggca gacaaaacgg
    15661 atatccggaa aacggcggaa gagaaagtga agaccgctga acaggagcag gcagtacctg
    15721 cttccccgtc agatttaggc accacggcct ccgtcggaat cggtgataat tttgaacggt
    15781 tacttggtgc ttctcattca gaggctgagg cgattatgcg tggtatgacg aatgctgaaa
    15841 tagacgcact tctggagaag ctcgggtaac ttactgaagg agtactgaag taatgacgac
    15901 tgtaacatca gcccaggcga ataagctgta tcaggtggcg ctttttactg ctgccaaccg
    15961 caaccgctcg atggttaata tccttactga acagcaggaa gcgccaaaag cggtttcgcc
    16021 ggacaagaaa agcacgaagc agaccagcgc aggtgcgccg gttgtccgta tcacagacct
    16081 taacaaacag gccggtgatg aagtgacctt cagcatcatg cacaaactct caaaacgtcc
    16141 gacgatggga gatgagcgtg ttgaaggtcg tggtgaggat ctcagccatg ctgacttctc
    16201 cctgaaaatc aatcagggac gtcacctggt ggatgcaggc ggacgtatga gtcagcagcg
    16261 cacgaagttt aacctggcat cctctgccag aacgcttctg gggacgtact ttaatgacct
    16321 gcaggaccag tgtgcgatag tgcatcttgc tggagctcgt ggtgattttg ttgctgacga
    16381 cactattctg ccgacagcg
//
LOCUS       DAZGAN020000107        16041 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGAN020000107 DAZGAN020000000
VERSION     DAZGAN020000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.107"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..79)
                     /gene="yojO"
                     /locus_tag="ACHKYO_RS19095"
                     /old_locus_tag="pgaptmp_003819"
     CDS             complement(<1..79)
                     /gene="yojO"
                     /locus_tag="ACHKYO_RS19095"
                     /old_locus_tag="pgaptmp_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HGU4430589.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFA"
     gene            188..682
                     /gene="napF"
                     /locus_tag="ACHKYO_RS19100"
                     /old_locus_tag="pgaptmp_003820"
     CDS             188..682
                     /gene="napF"
                     /locus_tag="ACHKYO_RS19100"
                     /old_locus_tag="pgaptmp_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HGU4430590.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYDCAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            672..935
                     /gene="napD"
                     /locus_tag="ACHKYO_RS19105"
                     /old_locus_tag="pgaptmp_003821"
     CDS             672..935
                     /gene="napD"
                     /locus_tag="ACHKYO_RS19105"
                     /old_locus_tag="pgaptmp_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HGU4430591.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            932..3418
                     /gene="napA"
                     /locus_tag="ACHKYO_RS19110"
                     /old_locus_tag="pgaptmp_003822"
     CDS             932..3418
                     /gene="napA"
                     /locus_tag="ACHKYO_RS19110"
                     /old_locus_tag="pgaptmp_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HGU4430592.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPG
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3425..4120
                     /gene="napG"
                     /locus_tag="ACHKYO_RS19115"
                     /old_locus_tag="pgaptmp_003823"
     CDS             3425..4120
                     /gene="napG"
                     /locus_tag="ACHKYO_RS19115"
                     /old_locus_tag="pgaptmp_003823"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HGU4430593.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4107..4970
                     /gene="napH"
                     /locus_tag="ACHKYO_RS19120"
                     /old_locus_tag="pgaptmp_003824"
     CDS             4107..4970
                     /gene="napH"
                     /locus_tag="ACHKYO_RS19120"
                     /old_locus_tag="pgaptmp_003824"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HGU4430594.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGSLLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4967..5416
                     /gene="napB"
                     /locus_tag="ACHKYO_RS19125"
                     /old_locus_tag="pgaptmp_003825"
     CDS             4967..5416
                     /gene="napB"
                     /locus_tag="ACHKYO_RS19125"
                     /old_locus_tag="pgaptmp_003825"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HGU4430595.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5426..6028
                     /gene="napC"
                     /locus_tag="ACHKYO_RS19130"
                     /old_locus_tag="pgaptmp_003826"
     CDS             5426..6028
                     /gene="napC"
                     /locus_tag="ACHKYO_RS19130"
                     /old_locus_tag="pgaptmp_003826"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HGU4430596.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6041..6664
                     /gene="ccmA"
                     /locus_tag="ACHKYO_RS19135"
                     /old_locus_tag="pgaptmp_003827"
     CDS             6041..6664
                     /gene="ccmA"
                     /locus_tag="ACHKYO_RS19135"
                     /old_locus_tag="pgaptmp_003827"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HGU4430597.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            6661..7323
                     /gene="ccmB"
                     /locus_tag="ACHKYO_RS19140"
                     /old_locus_tag="pgaptmp_003828"
     CDS             6661..7323
                     /gene="ccmB"
                     /locus_tag="ACHKYO_RS19140"
                     /old_locus_tag="pgaptmp_003828"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HGU4430598.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7365..8102
                     /gene="ccmC"
                     /locus_tag="ACHKYO_RS19145"
                     /old_locus_tag="pgaptmp_003829"
     CDS             7365..8102
                     /gene="ccmC"
                     /locus_tag="ACHKYO_RS19145"
                     /old_locus_tag="pgaptmp_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HGU4430599.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8099..8308
                     /gene="ccmD"
                     /locus_tag="ACHKYO_RS19150"
                     /old_locus_tag="pgaptmp_003830"
     CDS             8099..8308
                     /gene="ccmD"
                     /locus_tag="ACHKYO_RS19150"
                     /old_locus_tag="pgaptmp_003830"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HGU4430600.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8305..8784
                     /gene="ccmE"
                     /locus_tag="ACHKYO_RS19155"
                     /old_locus_tag="pgaptmp_003831"
     CDS             8305..8784
                     /gene="ccmE"
                     /locus_tag="ACHKYO_RS19155"
                     /old_locus_tag="pgaptmp_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HGU4430601.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8781..10724
                     /gene="ccmF"
                     /locus_tag="ACHKYO_RS19160"
                     /old_locus_tag="pgaptmp_003832"
     CDS             8781..10724
                     /gene="ccmF"
                     /locus_tag="ACHKYO_RS19160"
                     /old_locus_tag="pgaptmp_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HGU4430602.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSIYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGITFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10721..11278
                     /gene="dsbE"
                     /locus_tag="ACHKYO_RS19165"
                     /old_locus_tag="pgaptmp_003833"
     CDS             10721..11278
                     /gene="dsbE"
                     /locus_tag="ACHKYO_RS19165"
                     /old_locus_tag="pgaptmp_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HGU4430603.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11275..12327
                     /gene="ccmH"
                     /locus_tag="ACHKYO_RS19170"
                     /old_locus_tag="pgaptmp_003834"
     CDS             11275..12327
                     /gene="ccmH"
                     /locus_tag="ACHKYO_RS19170"
                     /old_locus_tag="pgaptmp_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HGU4430604.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12362..13009)
                     /gene="narP"
                     /locus_tag="ACHKYO_RS19175"
                     /old_locus_tag="pgaptmp_003835"
     CDS             complement(12362..13009)
                     /gene="narP"
                     /locus_tag="ACHKYO_RS19175"
                     /old_locus_tag="pgaptmp_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HGU4430605.1"
                     /translation="MPEATPFQMMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13329..15920
                     /gene="yejO"
                     /locus_tag="ACHKYO_RS19180"
                     /old_locus_tag="pgaptmp_003836"
                     /pseudo
     CDS             13329..15920
                     /gene="yejO"
                     /locus_tag="ACHKYO_RS19180"
                     /old_locus_tag="pgaptmp_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         3555 a         3925 c         4685 g         3876 t
ORIGIN      
        1 gagcaaaagc ccgtttttgc cacgcttccc gccactcttt tgatcctgct acaggtttta
       61 ccccgatcgg ggtatgcatc tttgacacat cctttaatat cttagcggct ataaaaatgg
      121 cttattaatt atgcggttta tttggtcgct ctcaattttc agagcgcgtt aatgatggaa
      181 ggtcaatgtg aagattgatg catcccgtcg gggcatactc actggtcgct ggcgcaaagc
      241 cagtaacggt atccgtccgc cctggtccgg tgatgaatct cattttctga cccattgcac
      301 ccgctgcgac gcctgtatta atgcttgtga aaacaacatt ctgcaacgcg gcgcgggcgg
      361 ctatccgagc gttaatttca aaaataacga gtgcagcttc tgttacgact gcgcgcaggc
      421 ttgccccgaa tcgctatttt ctccgcgcca caccagggcc tgggatttgc agttcaccat
      481 tggggacgcg tgcctggcgt atcagtctgt tgaatgccgc cgctgtcagg atagctgtga
      541 gccaatggca attatctttc gcccgacgct ctccgggatc taccagccgc aacttaatag
      601 ccaactttgc aacggatgtg gagcatgtgc agccagctgt ccggtatcag ccataactgc
      661 ggagtatctc catgcacact aactggcaag tttgcagcct ggtcgtgcag gccaaaagcg
      721 aacgaatttc agacatcagc acccaactga acgcctttcc cggctgtgaa gttgctgtca
      781 gcgacgcgcc gagcggtcag ttgattgtgg tggtggaagc agaagacagc gaaacgctga
      841 tccaaaccat tgagtcagta cgcaacgtag agggcgtgct ggcggtgtcg ctggtttatc
      901 accagcagga agagcaaggt gaggaaacac catgaaactc agtcgtcgta gctttatgaa
      961 agctaacgcc gttgcggccg ctgcggcggc tgccggtctc agcgtgccgg gcgttgcccg
     1021 cgccgttgtt ggtcagcagg aagccattaa atgggataaa gcgccgtgcc gtttctgcgg
     1081 tactggttgc ggcgttctgg tcggaacgca gcaggggcgt gtggtggcct gtcagggcga
     1141 cccggacgca ccggttaacc gtggcctgaa ctgcattaag ggctatttcc tgcccaagat
     1201 catgtacggt aaagaccgtt tgacgcagcc gctgctgcgt atgaaaaacg gtaaatatga
     1261 caaagaaggc gaatttaccc caatcacctg ggatcaggcc ttcgatgtga tggaagagaa
     1321 attcaaaacc gccctgaaag aaaaaggacc ggaatcgatc ggtatgttcg gttctggtca
     1381 gtggactatc tgggaaggtt atgccgcgtc caagctgttc aaagcgggct tccgttcgaa
     1441 caacatcgac ccgaacgcac gtcactgtat ggcgtcggca gtagttggct ttatgcgtac
     1501 ctttggtatg gatgagccga tgggctgcta tgacgacatc gagcaggctg acgcgtttgt
     1561 gctgtggggc gctaacatgg cggagatgca cccgatcctc tggtcacgca tcactaaccg
     1621 tcgtctctct aaccagaacg tcaccgtggc ggtgctttct acctaccagc atcgtagctt
     1681 cgagctggcg gataacggca tcatctttac gccgcaatct gacctggtga tcctgaacta
     1741 catcgccaac tatatcattc aaaacaatgc gataaatcag gacttcttca gcaagcacgt
     1801 taacctgcgc aaaggggcga cggacatcgg ctacggttta cgtccgaccc atccgctgga
     1861 aaaagcagcg aagaatccgg gttctgacgc ctccgaaccg atgagctttg aagattacaa
     1921 agccttcgtt gctgagtata cgctggaaaa aaccgctgaa atgaccggcg tgccgaaaga
     1981 ccagttagaa caactggcgc agctgtatgc cgatccgaac aagaaagtca tctcctactg
     2041 gacgatgggc ttcaaccagc atacccgtgg tgtgtgggcc aacaacctgg tctacaacct
     2101 gcacctgctg accggcaaaa tttcccagcc gggttgcggt ccgttctccc tgaccgggca
     2161 gccttctgcg tgtggtactg cgcgtgaagt gggcaccttt gctcaccgtc tgcctgcgga
     2221 catggtggtg actaacgaga aacatcgtga tatctgcgag aagaagtgga atatcccggg
     2281 cggcaccatt ccggcaaaaa tcggtctgca cgcggtggca caagaccgtg cgctgaaaga
     2341 cggcaagctg aatgtttatt ggaccatgtg taccaacaac atgcaggccg ggccgaacat
     2401 taatgaagag cgtatgccgg gctggcgcga tccgcgcaac ttcatcatcg tctccgatcc
     2461 gtatccgaca gtcagtgcgc tggccgccga cttgatcctg ccgaccgcaa tgtgggtaga
     2521 gaaagagggc gcttacggta acgccgaacg ccgtactcag ttctggcgtc agcaagtaca
     2581 ggcaccgggc gaagcgaaat cggatctctg gcagttagtc cagttctccc gccgcttcaa
     2641 aactgaagaa gtatggccgg aagagctgct ggcgaagaaa ccggaactgc gtggcaaaac
     2701 gctgtacgaa gtgctgtatg ccacaccgga agtgagcaaa ttcccggtat ccgaactggc
     2761 ggaagatcag ctgaatgatg aatcccgcga gctgggcttc tatctgcaaa aagggctgtt
     2821 cgaagagtac gcatggtttg gtcgcggtca cggtcacgat ctggcaccgt tcgatgacta
     2881 ccacaaagcg cgcggtctgc gctggccggt ggtgaacggt aaagaaacgc agtggcgtta
     2941 cagcgaaggt aacgacccgt acgtgaaagc gggcgaaggc tataagttct acggtaaacc
     3001 ggatggtaaa gcggtgatct tcgcgctgcc gttcgaaccg gcggcggaag caccggatga
     3061 agagtacgac ctgtggctct ctaccggacg cgtcctggag cactggcaca ccggcagtat
     3121 gactcgccgt gtgccggaac tgcaccgcgc cttcccggaa gcggtcctgt ttattcaccc
     3181 gctggatgcg aaagcgcgcg atctgcgccg tggcgacaaa gtgaaagtgg tttctcgccg
     3241 tggcgaagtg atctcgattg tcgaaacgcg cggtcgtaac cgtccgccgc agggcctggt
     3301 gtacatgccg ttcttcgacg ccgcacagct ggttaacaaa ctaacgctgg acgcgaccga
     3361 tccgctctcg aaagagacgg acttcaagaa gtgcgcagtc aaactggaga aggtgtaagc
     3421 cattatgtcc cggtcagcga aacctcaaaa tggtcgccgc cgctttctgc gcgatgttgt
     3481 tcgcacagca ggcgggctgg ctgccgtggg tgtggcgctg gggttacaac agcaaaccgc
     3541 acgcgcatct ggcgtgcggt tgcgcccgcc cggagccata aacgagaacg cctttgccag
     3601 tgcctgtgtg cgttgcggtc agtgtgttca ggcttgccct tacgacacct taaaactggc
     3661 gacgctggct tctggcctgt cggcgggcac gccatatttt gtcgcacggg atattccttg
     3721 cgaaatgtgt gaggacattc cgtgcgccaa agtgtgccca agcggtgcgc tggatcgtga
     3781 gattgaatcg atcgacgacg cgcggatggg gctggcggta ctggtggacc aggaaaactg
     3841 tctcaacttt caggggctgc gctgcgatgt ttgttatcgc gaatgcccga aaattgatga
     3901 ggccatcacc ctggagctgg agcgcaacac gcgtaccggt aagcacgccc gctttctgcc
     3961 gacggttcac agcgacgcct gtaccggttg cggtaagtgc gaaaaagtat gcgtgctgga
     4021 acaaccggca atcaaggtgt taccgctgtc actggcgaaa ggggagttag gtcaccatta
     4081 ccgcttcggc tggctggagg ggaacaatgg caaatcgtaa acgtgacgcc gggcgcgagg
     4141 cgctggagaa aaaaggctgg tggcgcagtc accgttggct ggtgttacgt cgcctttgtc
     4201 agttcttcgt gctggggatg tttttgagcg gtccgtggtt tggtgtgtgg atcttacacg
     4261 gtaactacag cagtagcctg ttattcgaca ccgttccgct gaccgatccg ctgatgacgc
     4321 tgcaaagtct tgccagtggt catctgcctg ccacggtggc gttgaccggt gcggtgatta
     4381 tcaccgtgct ctacgccctg gcaggaaagc gattattttg cagctgggtc tgcccgctga
     4441 atccgattac cgacttagcg aactggttac gcaggcgctt tgacctcaat cagtctgcga
     4501 cgatccctcg ccacattcgc tacgtgctgt tggtggtcat tctgctggga tcggccttaa
     4561 ctggcacgct catctgggaa tggatcaacc ccgtatctct gatggggcgc agcctggtaa
     4621 tgggcttcgg cagcggctcg ctgcttattc tcgcgctgtt tttatttgat ctactggtcg
     4681 ttgaacacgg ctggtgcggg cacatttgtc cggttggcgc gctgtatggc gtgctgggta
     4741 gcaaaggtgt gattaccgtt gcggccagcg atcggcagaa atgtaaccgc tgtatggatt
     4801 gttttcatgt ttgcccggaa ccgcatgtgc tacgtgcccc ggtgctggat gaacaaagcc
     4861 cggtgcaggt caccagccgc gattgcatga cgtgcggtcg ctgcgtggat gtctgttctg
     4921 aggatgtatt tacaataact acacgatgga gttcgggagc gaaatcatga aaagccatga
     4981 cctgaagaaa gcgctgtgtc aatggacggc gatgctggcc ctggtggtaa gcggcgcggt
     5041 ttgggcggct aatggcgtcg attttagcca gtcgccggaa gtatccggga cacaggaagg
     5101 ggccattcgg atgccaaaag agcaggaacg gatgccgctg aactatgtga atcagccgcc
     5161 aatgatcccg catagcgttg aaggttatca ggtaacgacc aataccaacc gctgcttgca
     5221 gtgccacggt gtcgaaagtt atcgcaccac tggcgcgccg cgtatcagtc ctactcactt
     5281 tatggacagc gacggcaaag tgggcgcgga agtggctccg cgtcgttatt tctgtctgca
     5341 atgtcacgta ccgcaggccg ataccgcgcc aatcgtgggg aataccttta ccccatcaaa
     5401 aggttacggg aaataagagg ttattatggg aaattctgac cgtaagcctg gtctgattaa
     5461 gcgcctgtgg aaatggtggc gtacccccag ccgtctggcg ctggggacgc tgctgttgat
     5521 cggttttgtc ggcggcatcg tcttctgggg cggctttaac actgggatgg aaaaagccaa
     5581 taccgaagag ttctgcatta gctgccacga aatgcgtaac acggtgtatc aggaatacat
     5641 ggattccgtg cactacaaca accgtagcgg cgtccgtgcg acctgtccgg attgtcacgt
     5701 tccgcacgag tttgtgccga agatgatacg caagctcaaa gcaagtaaag agctgtatgg
     5761 taaaattttt ggcgttattg acacgccgca gaaatttgaa gctcatcgtc tgacgatggc
     5821 tcagaatgag tggcggcgca tgaaggacaa taactcgcag gagtgccgta actgtcacaa
     5881 cttcgagtat atggatacaa ccgcccagaa atcggttgcc gcgaagatgc atgaccaggc
     5941 ggtgaaagat gggcaaacct gtattgattg ccataaaggg atagcgcaca agctgcccga
     6001 tatgcgtgaa gtcgagccag gtttttaaca gggttattgc gtgggtatgc ttgaagccag
     6061 agagttactt tgtgagcggg atgaacgaac cttatttagt ggcttgtcat ttacgctgaa
     6121 cgcaggagag tgggtacaaa tcaccggtag caacggtgcg gggaagacaa cgcttctccg
     6181 tttgctgact gggttgtctc gccctgacgc tggcgaggtt ctctggcaag ggcagccctt
     6241 gcatcaggta cgcgacagct accatcaaaa cctgttatgg ataggccatc agccggggat
     6301 caaaacccgg ctgacggcgt tagaaaatct gcacttttat catcgcgatg gcgatgccgc
     6361 acaatgtctg gaagccctgg cgcaggccgg gcttgccgga ttcgaagata ttccggtaaa
     6421 tcaactttct gccgggcaac aacgccgcgt cgctttagcg cgtctgtggc tgacccgtgc
     6481 cacgttatgg atcctcgacg agccttttac cgcgattgac gttaacggcg tcgatcgtct
     6541 gacccagcgt atggcgcagc atacggagca gggggggatt gtgattctga ctacccacca
     6601 gccgctcaac gttgctgaaa gtaaaattcg ccgcatttca ctgacgcaaa cgggggccgc
     6661 atgatgttct ggcgcatttt ccgtcttgag ctgcgcgtag cgtttcgcca tagcgccgaa
     6721 atcgccaacc cgctgtggtt cttcctgatt gtaattaccc tttttccgct cagtatcggt
     6781 ccggagccgc aactgctggc gcgtattgca ccgggcatta tctgggttgc tgcgctgctt
     6841 tcatccttgc tggcgctgga acgactgttc cgtgacgatt tgcaggacgg cagtcttgaa
     6901 caattgatgt tgttgccgtt acccttgccc gccgttgtgc tggcgaaggt gatggcgcac
     6961 tggatggtaa ccggtctgcc gttactcatc ctttcgccac tggtagcaat gctactggga
     7021 atggatgttt atggctggca agtgatggcg ctgacgctgc tgctgggaac gcctacgctt
     7081 ggctttctcg gtgcaccggg tgtggcgttg actgtgggac ttaagcgcgg tggtgtgctg
     7141 ctcagcatac tggtgttacc gctgactatc ccattactca tctttgccac cgccgcgatg
     7201 gacgcggctt ccatgcattt gcccgttgac gggtatctgg caattttagg cgcgttgctg
     7261 gcaggcaccg cgacattaag tccttttgcg acggcggcag cgttacgaat cagcattcaa
     7321 taacgctgga ttactgattt ttgttcgagt ctggtatcga aactatgtgg aaaacactgc
     7381 atcaactggc gatcccacca cggctgtatc aaatctgtgg ctggtttata ccgtggctgg
     7441 caattgccag tgtggtcgtg cttaccgtcg gctggatctg gggattcggc tttgctccgg
     7501 ctgattatca gcagggaaat agctaccgca ttatctacct gcatgtgcct gcggcgatct
     7561 ggtcgatggg catttatgca tcaatggcgg tggcggcttt tattggcctt gtctggcaga
     7621 tgaaaatggc caacctggcg gtggcggcga tggcccccat tggtgccgtg tttaccttta
     7681 ttgccctggt taccggctct gcatggggaa aaccgatgtg gggcacctgg tgggtatggg
     7741 atgcacgtct gacttctgaa ctggtgctgc tgtttttgta tgtgggtgtg attgccctgt
     7801 ggcacgcctt cgacgaccgc cgtctggcgg gccgtgcggc aggtatcctg gtgctgattg
     7861 gcgtggtgaa tctgccgatt attcattact ccgtggagtg gtggaacacc ctgcatcagg
     7921 gatcaacgcg gatgcagcaa agtatcgatc cggcgatgcg ttcgccgctg cgctggtcga
     7981 tttttggctt cctgctgctg tctgccacgc tgacgctgat gcggatgcgt aatttgattt
     8041 tgctgatgga aaaacgccgt ccgtgggtga gtgaactgat actgaaaaga ggccgtaaat
     8101 gacccctgca tttgcttcct ggaatgaatt tttcgcaatg ggcggttacg ccttttttgt
     8161 ctggctggcg gtggtgatga ccgttattcc gctggtggtt ttggtcgtgc actcggtgat
     8221 gcagcatcgc gcaattctgc gtggtgtggc gcaacagcgg gcgcgtgagg cgcgtttacg
     8281 tgctgcgcaa cagcaggagg ctgcatgaat attcgccgta aaaaccgctt gtggattgcc
     8341 tgtgccgtgt tggcagggct ggcgctgact atcggtctgg tgctatatgc gctgcgctcg
     8401 aatatcgatc tcttttatac gccgggggaa attctctacg gcaagcgtga aactcagcaa
     8461 atgccggaag tcgggcagcg tctgcgcgtc ggcgggatgg tgatgccggg tagcgtgcag
     8521 cgcgatccca attcgctaaa agtaactttc accatttacg acgctgaagg ttcagtagat
     8581 gtctcttacg aaggcatttt gccggatctg ttccgtgaag ggcagggcgt tgtggtgcag
     8641 ggcgagctgg aaaaaggcaa tcatatcctc gcgaaagaag tgctggcgaa acacgacgaa
     8701 aactacacgc cgccagaagt tgagaaagcg atggaagcta accaccgtcg cccggcgagt
     8761 gtttataagg acccagcatc atgatgccgg aaattggaaa cgggctgctg tgcctggcgt
     8821 tagggattgc gctgctgctg tccgtgtatc cgctatgggg cgtggcgcgc ggagatgcgc
     8881 gcatgatggc gtcttcccgc ttgtttgcct ggctgctgtt tatgtctgtg gctggcgcat
     8941 ttctggtgct ggtcaatgct ttcgtggtca acgactttac cgtcacctat gttgccagca
     9001 actccaatac ccagcttccg gtgtggtatc gcgtggcggc tacctggggc gcgcatgaag
     9061 gctcgctact gctgtgggtg ctgctgatga gcggctggac ctttgcggtg gcgattttta
     9121 gtcagcgtat tccgctggat attgtggccc gcgtactggc gataatgggg atggtcagcg
     9181 tcggcttttt gctgttcatt ctctttacct ctaacccatt ctcacgcacg ctgccgaact
     9241 tcccgattga agggcgcgat cttaacccgc tgttacagga tccggggctg atcttccatc
     9301 cgcctctgct ttatatgggg tacgtgggtt tctcggtggc gtttgctttt gccattgctt
     9361 ctttgttgtg cgggcgtctg gacagcattt atgcgcgttt tactcgtccg tggacgctgg
     9421 cggcgtggat cttcttgacg ctcggcattg tgctcggttc cgcatgggcc tattacgaac
     9481 tcggctgggg cggctggtgg ttctgggatc cggtagaaaa cgcctcgttt atgccgtggc
     9541 tggtggggac tgcgctgatg cactcactgg cggtcactga acaacgcgcc agcttcaaag
     9601 cgtggacatt actgctggca atcagtgcct tctcgttgtg tctgctgggg actttcctgg
     9661 tgcgttccgg cgtgctggta tcggtacacg cgtttgcctc tgatccggca cgcggtatgt
     9721 ttatcctcgc ctttatggta ctggtgattg gtggttcgct gctgctgttt gccgcgcgtg
     9781 gacacaaagt tcgctcacgc gtaaacaatg cgctgtggtc gcgggaatct ctgctgttag
     9841 cgaacaacgt tttgctggtc gccgcgatgc tggtggtatt actggggacg ctgctgccgc
     9901 tggtgcacaa gcaactggga ctgggcagta tttcgattgg cgaaccgttc ttcaacacca
     9961 tgtttacctg gctgatggtg ccgtttgcgc tgctgcttgg tgtcggtcct ctggtgcgct
    10021 gggggcgcga tcgcccacgt aaaatccgca atttattgat tatcgccttc atctcaacgc
    10081 tggtgctgtc gctgctgctg ccgtggctgt tcgaaagcaa agttgtggcg atgacggtgc
    10141 tcggcctggc aatggcctgc tggattgctg tgctggcaat tgcggaagct gcgctgcgta
    10201 tttcacgtgg cacgaaaacc accttcagtt attggggaat ggtggcggct cacctggggc
    10261 tggcagtgac aattgttggt attaccttta gccagaacta tagcgttgag cgtgatgtgc
    10321 gcatgaagtc cggcgatagc gtcgatattc atgaatatcg cttcaccttc cgtgatgtca
    10381 aagaggtgac tggcccgaac tggcgtggcg gtgtggcgac tatcggcgta acgcgcgatg
    10441 gcaagccgga aacggtgctg tatgcggaaa aacgttatta caacactgcc gggtcgatga
    10501 tgaccgaagc ggcaattgac ggcggcatca cgcgtgacct gtacgccgcg ctcggtgaag
    10561 agctggaaaa cggcgcgtgg gccgtgcgtc tttactacaa accatttgtt cgctggattt
    10621 gggcgggcgg gctgatgatg gcgttgggcg gactgctgtg tctgtttgat cctcgctatc
    10681 gtaagcgcgt gagtccgcaa aaaactgcgc cggaggccgt atgaagcgca aagtattgtt
    10741 aattccgttg attatcttcc tggcgattgc cgcggcgctg ctgtggcagc tggcgcgtaa
    10801 tgccgaaggg gatgatccga ccaatctgga atcggcgctc attggcaagc ctgtgccgaa
    10861 gtttcgtctc gaatcactgg acaatccggg gcagttttat caggcggatg tgctgactca
    10921 gggcaaacca gtgctgctta acgtctgggc gacctggtgt ccgacctgcc gtgcggaaca
    10981 tcaatatctg aatcagcttt cagcgcaggg cattcgcgtg gtcggcatga actataaaga
    11041 cgatcgccag aaggcaatca gctggctgaa agagctgggc aatccttacg cgctaagcct
    11101 gtttgatggc gacggcatgt tagggctgga tctcggtgtc tatggcgcgc cagaaacctt
    11161 ccttattgac ggcaacggta tcattcgcta tcgccatgcg ggcgatctca atcctcgcgt
    11221 ctgggaagaa gagatcaagc cgctgtggga gaaatacagt aaggaggccg cacaatgagg
    11281 tttttattgg gcgtgctgat gctgatgatc tccggctcag cactggcgac catcgacgtg
    11341 ttgcagttta aagatgaagc acaggaacaa cagttccgtc agctcactga agaactgcgc
    11401 tgcccgaaat gccagaacaa cagcattgcc gattccaact cgatgattgc caccgacctg
    11461 cgtcagaaag tgtatgaact gatgcaggaa ggtaaaagta aaaaagagat tgtcgattat
    11521 atggtggcgc gttacggcaa cttcgtcact tacgatccgc cgttaacgcc gctgaccgtg
    11581 ctgctgtggg tgctgccggt agtggctatt ggcattggcg gttgggtcat atacgcccgc
    11641 tcgcggcgtc gggtacgcgt agtgccggaa gcgtttcctg aacaaagcgt gcaggaaggt
    11701 aagcgtgccg gatatattgt ttatctgccg ggtattgtgg tggcgttaat tgtggctggc
    11761 gtcagctact accagactgg caattatcag caggtgaaaa tctggcagca ggccacggca
    11821 caggctccgg cgttgctgga cagggcgctg gatccgaaag ccgatccgct caacgaagaa
    11881 gagatgtcgc gtcttgcgct ggggatgcgt actcaactgc aaaaaaatcc gggagatata
    11941 gaaggctgga ttatgttggg ccgcgttggc atggcgctgg gtaacgctag tatcgccacc
    12001 gatgcatacg ctactgcgta tcgcctcgat ccgaaaaaca gcgatgccgc actgggttat
    12061 gctgaagcgt tgacacgttc atctgatccc aacgacaacc gcctcggtgg tgaactgctg
    12121 cgccagttgg tgagaagcga ccacagcaat atccgtgtgt taagcatgta tgcgtttaat
    12181 gcctttgagc agcagcgatt tggcgaagcc gttgccgcgt gggagatgat gttgaaactc
    12241 ttacctgcca acgatactcg ccgtgcggtg attgaacgta gtatcgcgca ggcgatgcaa
    12301 catttgtcgc cgcaggagag taaataaaaa gaggcccgat ggtgatgcca tcgggctatt
    12361 tttattgtgc cccgcgttgt tgcaggaaca gaatggtggc tgccacgcgt gagcggacat
    12421 tgagtttgcg cagcagattg cgaatatgta cttttactgt ctgctctgaa atattcaaca
    12481 ccgaggcaat ctgtttattt gacagcccct gtgccagctc gtgcagaaca tccagctcgc
    12541 gctccgtcag cacgctgaag ggatcttctt ccgcgccaaa catttcacgt tcacgtaagt
    12601 actgattgac gcgttcgcta aagactttgc tgcctttcgc tccagcacga attgcttcca
    12661 gcaatacttc cgggtcgctg tctttcaaca gataaccgtc tgcgcctgcg tctatcagtg
    12721 caaagacatc gctggaggca tctgatacgg tcaggataat aatttgcgcg gtaacgccat
    12781 ccctgcgcaa ggcattgagg gtatccaggc cactcatacc tttcatattg agatccagca
    12841 agatcacgtc gatatccagt ctattcgcca gatcgatcgc gctcgcgccg tcgcccgctt
    12901 cggcgaccac ttcaaagcca ggatcaagct ccagtaactg acgaacaccg cgtcgcataa
    12961 gtggatgatc atccacaatc atcatctgaa aaggcgttgc ttcaggcata gtagtctcct
    13021 gaggttttat tagaatcatt atgtttgagc gtagcgttgg taacgctacc aggcgcgtga
    13081 atgttacccc attttatgta tggacatgta gtgaaaacta caaccttgtc gttcgctttg
    13141 gcatcatttt cattataaag acggaatttt gcatcagctt ctcatcaaga aaaacaacta
    13201 aaaaatcaat cacaatactt gtgcatttct ttaaacagtt tttaaaaact gactccgggt
    13261 atggagctat gggtattttc cgtacccaat gcttttaaca gcaattaatt tcataggatg
    13321 aaagctcaat tcatcaatct ggttctgttt ctctttgtcg ttccgcaata tctgttctgg
    13381 tggctacagc gttatattca cccatagcat tggcatcaac agttgagtat ggtgagacag
    13441 ttgatggtgt tgtcctggaa aaagatatcc agctggttta tgggaccgcc aataatacga
    13501 aaatcaatcc tggcggagaa cagcatataa aagaatttgg tgtaagtagt aatactgaaa
    13561 ttaaaggagg gtatcagtac attgaaatga atggtaccgc agaatactca gtattaaatg
    13621 acggttatca aattgttcaa atgggtggcg cggcaaacca gactaccctc aataatggtg
    13681 tgctacaggt ttatggcgca gcgaatgatc ccacgattaa aggcgggcgc ttaatcgttg
    13741 aaaaagatgg gattaccgtc cttgccgcta tcgaaaaggg aggattactg gaggttaaag
    13801 aggggggatt agcgattgcg gtagatcaga aagcaggcgg tgctattaaa gcaagcacgc
    13861 gggtcatgga ggcattcgga acaaaccgtc tcggtcagtt cgaaatcaag aatggtattg
    13921 ctaacaatat gctgttggaa aacggcggaa gtttgcgagt tgaagaaaat gacttcgctt
    13981 ataatactac tgtagatagt ggcggcttac tggaggttat ggatggcggg actgtaactg
    14041 gcgttgataa aaaagcaggc ggaaaattaa ttgtctcaac gaatgcgctg gaagtgagtg
    14101 gtacaaacag taaaggccaa tttagtataa aagatggtgt gtcaaaaaat tatgaactgg
    14161 atgatggttc cgggctcatt gttatggagg acacgcaggc cattgatact atccttgatg
    14221 agcatgccac tatgcaatcg ctgggaaagg atactggtac gaaagtgcag gcaaatgcgg
    14281 tatatgatct cggtcgatca gatcagaatg gaagtatcac gtattcctca aaagccattt
    14341 ctgaaaatat ggttatcaac aatggccgcg ctaacgtctg ggctggcaca atggttaacg
    14401 tttcagtcag agggaatgat ggcattcttg aggtcatgaa gccgcaaata aattatgcac
    14461 ccgcaatgtt ggtgggtaag gtagtggttt ctgagggcgc ttcttttaga acgcatggtg
    14521 ccgtggatac cagcaaagcg gacgtttcgc tcgaaaatag cgtatggacc atcattgccg
    14581 atatcactac gacgaaccaa aacaccctcc tcaacttagc caaccttgcg atgtctgacg
    14641 caaatgtgat tatgatggat gagccagtga ctcgttcatc agtgacggca agtgcggaaa
    14701 atttcattac gttgaccacc aataccctgt cgggaaacgg caatttttat atgcgtaccg
    14761 atatggctaa tcatcagagc gatcagctca acgtcaccgg tcaggcaaca ggtgatttca
    14821 aaatattcgt gacggacacc ggtgccagcc cggcagcagg agatagcctt acactggtaa
    14881 caacgggcgg cggtgatgct gcatttacgt tgggcaatgc cggaggcgtt gttgatatcg
    14941 gtacgtatga atataccttg ctggataatg gcaaccatag ctggagtttg gcagagaatc
    15001 gcgcgcaaat taccccttca accactgatg tgctgaatat ggcggcagca caaccgctgg
    15061 tatttgatgc agaactggac accgtgcgtg agcgtcttgg tagcgtaaaa ggcgttaatt
    15121 acgatacggc gatgtggagt tcggcaatta acacccgcaa caacgtgacc actgatgcgg
    15181 gcgctggctt tgagcaaaca ttgaccgggc tgacgctcgg tatcgatagc cgtttctccc
    15241 gtgaagaaac cagcacaatt cgcggcttgt tctttggtta ctctcattct gatattggtt
    15301 ttgatcgcgg cggcaaaggc aatgtcgata gctataccct gggggcttat gccggttggg
    15361 agcatcagaa cggtgcctat gttgatggag tggtgaaagt tgaccgtttt gccaacacca
    15421 tccattgcaa gatgagtaat ggggcaacag cgtttggcga ttacaatagt aacggcgcgg
    15481 gtgctcatgt cgagagcggg ttccgttggg ttgacggatt gtggagtgtt agaccctatc
    15541 tggcctttac cggctttacc acagatggtc aggactacac gttatcaaac ggcatgcgcg
    15601 cggatgtggg aaatacccgg atattacgcg ctgaagcggg aacggcggta agctatcaca
    15661 tggacctgca aaacggtacg acgctggaac cctgactgaa agccgccgtg cgtcaggaat
    15721 acgccgattc taaccaggtg aaagttaatg acgatggcaa atttaataat gatgtggctg
    15781 gaacccgtgg cgtttatcag gctgggataa ggtcatcgtt taccccgacg ttaagcggtc
    15841 atttgtcagt cagctatggc aatggcgcag gggtagaatc gccgtggaat actcaggcgg
    15901 gtgtggcctg gacgttctga taacaaaaaa taaacaggct gtgatgtgtc acggtctgtt
    15961 tatcgaatta attgcagata taaaaaaaac caaccgtaaa ggttgatttt ttcttgggat
    16021 ttttggcacg agagtagttt a
//
LOCUS       DAZGAN020000108        16013 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGAN020000108 DAZGAN020000000
VERSION     DAZGAN020000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.108"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..209
                     /locus_tag="ACHKYO_RS19185"
                     /old_locus_tag="pgaptmp_003837"
     CDS             <1..209
                     /locus_tag="ACHKYO_RS19185"
                     /old_locus_tag="pgaptmp_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430606.1"
                     /translation="VCGDNQKRYTPGFTALTERGVVPSWSLVRCDGGRNWILLQLITI
                     IILAGPFRRSGPLRGGDLADFHYL"
     gene            371..880
                     /locus_tag="ACHKYO_RS19190"
                     /old_locus_tag="pgaptmp_003838"
     CDS             371..880
                     /locus_tag="ACHKYO_RS19190"
                     /old_locus_tag="pgaptmp_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4430607.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            852..2780
                     /locus_tag="ACHKYO_RS19195"
                     /old_locus_tag="pgaptmp_003839"
     CDS             852..2780
                     /locus_tag="ACHKYO_RS19195"
                     /old_locus_tag="pgaptmp_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU4430608.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVAGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTITLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEAQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAADNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPSR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDVAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2764..2970
                     /locus_tag="ACHKYO_RS19200"
                     /old_locus_tag="pgaptmp_003840"
     CDS             2764..2970
                     /locus_tag="ACHKYO_RS19200"
                     /old_locus_tag="pgaptmp_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HGU4430609.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAELVLGTTRRRRRPLGVRL"
     gene            2967..4559
                     /locus_tag="ACHKYO_RS19205"
                     /old_locus_tag="pgaptmp_003841"
     CDS             2967..4559
                     /locus_tag="ACHKYO_RS19205"
                     /old_locus_tag="pgaptmp_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4430610.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPSQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGIGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNSVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            4549..6054
                     /locus_tag="ACHKYO_RS19210"
                     /old_locus_tag="pgaptmp_003842"
     CDS             4549..6054
                     /locus_tag="ACHKYO_RS19210"
                     /old_locus_tag="pgaptmp_003842"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4430611.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPEEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREHLA
                     TMLAGQQGMSVEQARAILTAAAPQQPVASTQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEKLAAFPGMTVEAARDILS
                     SSPDKAEPVSASTTAMFERIMANHSPAAVQGGVSQTSADGDADVKMLMAMP"
     gene            6091..6438
                     /locus_tag="ACHKYO_RS19215"
                     /old_locus_tag="pgaptmp_003843"
     CDS             6091..6438
                     /locus_tag="ACHKYO_RS19215"
                     /old_locus_tag="pgaptmp_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU4430612.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAAGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFVGTALSHAALP"
     gene            6496..7524
                     /locus_tag="ACHKYO_RS19220"
                     /old_locus_tag="pgaptmp_003844"
     CDS             6496..7524
                     /locus_tag="ACHKYO_RS19220"
                     /old_locus_tag="pgaptmp_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4430613.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPIVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7576..7959
                     /gene="gpFI"
                     /locus_tag="ACHKYO_RS19225"
                     /old_locus_tag="pgaptmp_003845"
     CDS             7576..7959
                     /gene="gpFI"
                     /locus_tag="ACHKYO_RS19225"
                     /old_locus_tag="pgaptmp_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4430614.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAV
                     SPEGIMHSDESGADQNHTDDGEQLNNTDAPDDVKAVRVRKCLQVMGYCPETGRPVELA
                     LRGMRVLVPSSLATAMIQHGTAEYA"
     gene            7952..8305
                     /locus_tag="ACHKYO_RS19230"
                     /old_locus_tag="pgaptmp_003846"
     CDS             7952..8305
                     /locus_tag="ACHKYO_RS19230"
                     /old_locus_tag="pgaptmp_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4430615.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEIFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            8321..8854
                     /locus_tag="ACHKYO_RS19235"
                     /old_locus_tag="pgaptmp_003847"
     CDS             8321..8854
                     /locus_tag="ACHKYO_RS19235"
                     /old_locus_tag="pgaptmp_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4430616.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     KELAYALKQQLRIAIKR"
     gene            8851..9246
                     /locus_tag="ACHKYO_RS19240"
                     /old_locus_tag="pgaptmp_003848"
     CDS             8851..9246
                     /locus_tag="ACHKYO_RS19240"
                     /old_locus_tag="pgaptmp_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4430617.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            9254..10006
                     /locus_tag="ACHKYO_RS19245"
                     /old_locus_tag="pgaptmp_003849"
     CDS             9254..10006
                     /locus_tag="ACHKYO_RS19245"
                     /old_locus_tag="pgaptmp_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4430618.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEDGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VTAIKVTPTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMVITVTG
                     IKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
     gene            10020..10451
                     /locus_tag="ACHKYO_RS19250"
                     /old_locus_tag="pgaptmp_003850"
     CDS             10020..10451
                     /locus_tag="ACHKYO_RS19250"
                     /old_locus_tag="pgaptmp_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4430619.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQRTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            10478..10891
                     /locus_tag="ACHKYO_RS19255"
                     /old_locus_tag="pgaptmp_003851"
     CDS             10478..10891
                     /locus_tag="ACHKYO_RS19255"
                     /old_locus_tag="pgaptmp_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4430620.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRTHYFQDTQLDMHFSGLMY
                     AVLSLFFCDPDMHPSDFSLLAPRREEAQTEMPDEEDMLMQKAAGLAGGVRFGGEGGGD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPAGW"
     gene            10872..13445
                     /locus_tag="ACHKYO_RS19260"
                     /old_locus_tag="pgaptmp_003852"
     CDS             10872..13445
                     /locus_tag="ACHKYO_RS19260"
                     /old_locus_tag="pgaptmp_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4430621.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLENDARKTAGAV
                     EQSLNRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSEFNKTLVLSGNQA
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAERESSQLKYTGEAQKAYECLLTPLEKYTARQEELNKALKDGKILQADYNTLMASAK
                     KDYESTQKKPSGVKVSAGERQEDRAHAAMLALETELRTLEKHSGVNEKISQQRRDLWE
                     AENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKVEHQKRLNELAQQA
                     ARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLRDVYGDNPDALAKATSALKNTWS
                     AEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQNLAAMLTGSEQNWR
                     GFTRSVLSMLTEILLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQAAAANFHFATG
                     GFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGAAGSPAQMR
                     RAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYEMARKGAQDELRLQLRDGGLLSGSG
                     R"
     gene            13442..13771
                     /locus_tag="ACHKYO_RS19265"
                     /old_locus_tag="pgaptmp_003853"
     CDS             13442..13771
                     /locus_tag="ACHKYO_RS19265"
                     /old_locus_tag="pgaptmp_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4430622.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            13771..14469
                     /locus_tag="ACHKYO_RS19270"
                     /old_locus_tag="pgaptmp_003854"
     CDS             13771..14469
                     /locus_tag="ACHKYO_RS19270"
                     /old_locus_tag="pgaptmp_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4430623.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            14480..15223
                     /locus_tag="ACHKYO_RS19275"
                     /old_locus_tag="pgaptmp_003855"
     CDS             14480..15223
                     /locus_tag="ACHKYO_RS19275"
                     /old_locus_tag="pgaptmp_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4430624.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMEGT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            15220..15801
                     /locus_tag="ACHKYO_RS19280"
                     /old_locus_tag="pgaptmp_003856"
     CDS             15220..15801
                     /locus_tag="ACHKYO_RS19280"
                     /old_locus_tag="pgaptmp_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4430625.1"
                     /translation="MNTAAEAIRALSMQVPGFRRQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
BASE COUNT         3677 a         3828 c         5083 g         3425 t
ORIGIN      
        1 gagtgtgcgg ggataatcaa aaacgataca caccggggtt taccgcgtta acggagcgcg
       61 gcgttgtccc ctcatggtcg ctggtccggt gcgatggtgg aagaaactgg attttgttgc
      121 aactgataac cattatcatt ttagcgggtc ctttccggcg atccgggccg ttacggggcg
      181 gcgacctcgc ggattttcac tatttatgaa aattttccgg gatccatgtc cggtttctct
      241 gcaagttaac catatgaaaa atataaaaac atgctttcca tgaaccggac atgcgcaaaa
      301 aatagacact aaaaccggac atgaccggtt ttgttgtgat tgtgaggtga gagtttttgc
      361 gaggtgagga gtggctacgc agactgaagt tgccaggcat ttaagtctga ccgatcgcca
      421 gcttcgcaga ttgcagaaat tgccgggtgc cccgatatcg aataagcgag ggcaactgga
      481 tctggatgcc tggcgcgatt tttacatatc gtatctgaga agaagtaaaa acgatgtgcc
      541 tgatggcgat agcgaagacg actatgagga gaaattgctt attgccagat gggaactgac
      601 agcagaacag gctgttacac agcagttaaa aaatgaggtg tcaaaaggaa aacttattga
      661 caccgggttc tgtatttttg ccctcagcaa gctggcaatg gcgttatcca gtacgcttga
      721 ttccatccct ttatccatgc agcgacagtt tcctgattta acaccgcgcc atcttgacca
      781 tctgaaaacc cttattgcga agggggcaaa tcagtgtgcg cgggcggggg ataaattacc
      841 ggatttactc gatgaatata tcagagcaac aactgaataa tatgatgagt gctgtcacaa
      901 cagcattaca gcccctgata agggcattgc cggtgacgcc agttgaatgg gctgatcaaa
      961 attattatct gcctaaagaa tcttcatatg gtgagggaga atggaaaacg ctgccgttcc
     1021 agatcgccat catgaacagc atggggaatg atcagatccg gactgttaat ctgattaaat
     1081 ctgcccgtgt tggctataca aagatgttgc tgggagtcgc cgggtatttt attgagcata
     1141 aatcccgaaa cagtctgctt tttcagccca cggattctgc cgctgaagat tttatgaagt
     1201 ctcacgtgga ggcgacgatt cgcgatgttc cctgcctgaa aaaactttct ccctggctgg
     1261 gacgtaaaca tcgtgataat accatcacgc tgaaacgctt ttcatcgggt gtgggcttct
     1321 ggtgcctggg cggcgctgcc gccaaaaact accgtgaaaa atccgtggac gtggtctgct
     1381 atgacgaact ttcctcgttc gagccggatg tcgaaaaaga gggctcgcca accctgctgg
     1441 gggataagcg tattgagggc tcggtatggc caaaatccat tcgcggctcg acgcctaaaa
     1501 taaaaggctc ctgccagatc gaaaaagcgg ccaacgagtc ggcgcatttc atgcgttttt
     1561 atgtgccctg cccgcactgt ggggaggcgc agtatctgaa atttggcgat gagtccacgc
     1621 cttttggcct taaatgggag aaggacagcc ccgaaagcgt tttctacctc tgtgaacatc
     1681 atggctgcgt gatccatcag tctgagcttg accagagcaa cgggcggtgg atctgtgaaa
     1741 acacggggat gtggacccgc gacggtctga cgtttttcag cgccgcggat aatgaaattc
     1801 cgccgccgcg ctccatcaca ttccatatct ggacggcgta cagtccgttc accacctggg
     1861 tacagattgt ctatgactgg ctggatgcac tgaaagatcc caacggcctg aaaacctttg
     1921 tgaacaccac gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg
     1981 tgctgatgga taaggttgtg cgttacacgg ctgcggtgcc ttcccgggtg gtttatctga
     2041 cggcgggcat tgactcgcag cgaaaccgtt ttgagatgta tgtctgggga tgggctccgg
     2101 gagaggaagc ctttctggtg gataaaatca tcattatggg gcgtcccgat gaggaagaga
     2161 cgctgttacg tgtggatgtg gcgatcaaca aaaaataccg ccatgcagac ggaaccgaaa
     2221 tgaccatttc ccgtgtctgc tgggacaccg gggggatcga tggcgaaatt gtctatcaga
     2281 ggtcaaaaaa acacggtgtt ttccgggtgc tgccggtaaa aggtgcatct gtttatggca
     2341 agccggtgat caccatgcca aaaacccgca atcagcgggg cgtgtatctg tgcgaagtgg
     2401 ggacggacac cgcaaaagaa attctctatg cccgtatgaa agccgatccc acgcctgcgg
     2461 atgaagccac gtcgtatgcc atccgttttc ctgatgatcc ggagattttt tcgcagacag
     2521 aggcgcagca actggtggcg gaagagcttg tggagaagtg ggaaaaagga aagatgcgtc
     2581 tgctgtggga taacaaaaag cggcgtaacg aagcgctgga ctgcctggtg tatgcctacg
     2641 cggcattacg tgtgtccgtg caacgctggc aacttgatct ggctgtactg gcaaaatccc
     2701 gggaagaaga gacgacccgg ccaaccctta aagaactggc agcgaagctg tccggaggag
     2761 tgaatggtta cagtcgctga actgcaggcg ctgcgtcagg cgcgccttga tttattaacc
     2821 ggtaaacggg tggtgtctgt ccagaaagat ggtcgcagaa ttgaatatac ggcggcctct
     2881 ctggatgagc ttaaccgggc gatcaatgat gctgagttgg tactggggac aacccgccgt
     2941 cgccgtcgtc cgctgggagt gaggttatga aacgaacgcc tgtcctgatt gatgtgaacg
     3001 gcgttccgct tcgggagagc ctcagctaca acggtggcgg tgcaggattt ggcgggcaaa
     3061 tggcagagtg gttgccaccc tcgcagagtg ccgatgcggc cctgctgccc gcgttgcgtc
     3121 tggggaatgc ccgtgcagat gatctggtgc gcaataacgg aatagcggcc aatgcggtgg
     3181 ccctgcataa ggatcacatt gtcgggcata tgtttctgat tagctaccgt ccgaactggc
     3241 gctggctggg gatgcgggag accgcggcaa aaagttttgt cgatgaggtg gaggcggcct
     3301 ggtcagaata cgcagaaggg atgtttggtg agatcgacgt ggaagggaaa cgcacgttta
     3361 cggaatttat ccgtgaaggt gtgggcgttc atgcgtttaa cggcgaaatc tttgtgcagc
     3421 cggtctggga tacggagagc acgcaactgt ttcgtacgcg ttttaaagcc gtgagtccga
     3481 aacgggtgga cacgccagga cacggtatcg ggaaccgttt tctgcgggcc ggtgtggagg
     3541 ttgatcgata tggccgtgcc gttgcgtacc atatctgtga ggatgatttt cctcgctccg
     3601 ggagtggacg atgggaacgg atcccgcgtg aacttcccac cgggcgtccg gccatgctgc
     3661 atattttcga gccggtggag gacgggcaga cccgtggggc caatcagttt tacagcgtca
     3721 tggaacggct gaagatgctc gattccctgc aggcaacaca gcttcagtcg gccattgtta
     3781 aagccatgta tgcagcgacg attgaaagtg aacttgatac cgaaaaggcc tttgaatata
     3841 tcgccggtgc gccgcagggg cagaaggata atccgcttat taatattctg gataagttct
     3901 ccacctggta tgacacgaat agcgtgacgc tgggcggtgt caaaattccg caccttttcc
     3961 ccggtgatga tctgaaactt cagaccgcgc aggattcaga caatggattt tcggcgcttg
     4021 aacaggcgct gctgcggtat atcgctgccg gtcttggcgt ttcctacgaa cagttgtccc
     4081 gtgattactc gaaggtcagt tattcaagtg cccgcgcatc cgccaatgag tcgtggcgct
     4141 attttatggg gcggcgaaaa tttattgcgt cccggctggc cacgcagatg ttttcctgct
     4201 ggctggaaga ggcacttctt cgggggatta ttcgtccgcc acgggcacgt tttgattttt
     4261 atcaggcgcg ttcagcctgg tcacgggctg agtggattgg tgccggaaga atggccattg
     4321 acgggctcaa ggaagtccag gaatcagtga tgcgcattga ggccggactg agcacgtatg
     4381 agaaagagct ggcgctgatg ggcgaggatt atcaggacat tttccgccag caggtcaggg
     4441 aatctgctga gcggcaaaaa gccggactct cacgtccggt gtggatagcg caggcgtatc
     4501 agcagcagat agcggagagt cgcaggccgg aagaggagac aacaccacgt gagacgtaat
     4561 ctttcacaca ttattgccgc agcattcaat gaaccgctgc ttctggagcc cgcctatgcg
     4621 cgggttttct tttgcgcgct cgggcgcgag atgggggcag caagtctttc ggtaccacaa
     4681 cagcaggtac agcttgatgc acccggaatg ctggctgaaa cggacgagta catggccgga
     4741 ggtaaacgac cggcccgtgt ttaccgggtg gtgaacggta ttgctgtact gccggtgaca
     4801 ggcacgctgg tgcaccggct ggggggtatg cggccatttt ccggaatgac aggctatgac
     4861 ggcattgtcg cctgtcttca gcaggcaatg gcggatagcc aggtgcgggg cgtactgctg
     4921 gacattgaca gtccgggcgg gcaggccgcc ggtgcgtttg actgtgctga catgatttac
     4981 cgcctccgtc agcagaagcc ggtctgggca ctgtgcaatg acacggcctg ttctgcagcc
     5041 atgctgctgg cgtcggcctg ctcccgacgg ctggttaccc agacatcccg tatcggctcc
     5101 attggcgtga tgatgagcca tgtcagctat gccggtcatc tggcgcaggc cggtgtggat
     5161 atcacgctga tttactcagg ggcgcacaag gtggatggca atcagtttga agcgttgccg
     5221 gaagaggttc gccaggacat gcagcagcgg attgatgcgg cgcgccggat gtttgccgaa
     5281 aaagtggcga tgtataccgg tctgtctgtt gatgcagtca cgggaacaga ggccgccgtt
     5341 tttgaaggtc agtccggcat tgaggccggg ctggcggatg aattaatcaa tgcgtcggat
     5401 gccatcagcg tgatggctgc ggcgctgaac acacatgata caggaggcac tatgccgcaa
     5461 ttaactgcaa cggaagctgc cgcgcaggag aaccagcgag tgatggggat cctgacgtgt
     5521 caggaagcga aaggacgtga acaccttgcc acgatgctgg caggacaaca gggcatgagc
     5581 gttgaacagg cccgggcgat tctgaccgcg gcagcaccac agcagccggt ggcatccacg
     5641 cagagtgaag ccgatcgcat tatggcgtgt gaagaggcta aaggtcgtga acaactggca
     5701 gcaacgctgg cggcgatgcc ggagatgacg gtggaaaaag cccgcccgat cctggcggct
     5761 tcaccgcagg cggatgccgg accctcactc cgtgatcaga ttatggctct ggatgaggca
     5821 aaaggggctg aggcgcaggc tgaaaaactg gcggcgtttc ccggaatgac ggtggaggct
     5881 gcccgcgaca ttctctcctc atcgccggat aaagcagaac cggtttctgc atccacaacc
     5941 gccatgtttg aacgcatcat ggcgaaccat tcaccggcag ccgtgcaggg tggcgtgtca
     6001 cagacgtcgg cagacggtga tgcggacgtg aaaatgctca tggccatgcc atgaagtcag
     6061 tgctgaccat caatatgagg tttttacaat atggtaacga aaaccatcac tgaacaacgt
     6121 gcagaagtac gtatttttgc cggtaatgat ccggctcata ccgccacagg cagcagcggg
     6181 atttctcagg caacaccggc actgacgccc ctgatgctgg atgaagccag cgggaaactg
     6241 gtggtctggg acggacagaa agccggtagt gcggctggca tactggtact gccgcttgaa
     6301 ggcacagaga cggtgctgac gtattacaag tcggggacct ttgcgacgga ggcaatccgc
     6361 tggcctgaaa gtgtggatga acacaaaaag gccaacgcct ttgtcggcac agccctgagt
     6421 cacgcggcgc tgccgtaaca cgttatcagg ccaccgcgtt ggcctgactg atttctgaat
     6481 gaaaggaact gatttatggg attgtttacg acccgccagt tactcggtta taccgaacaa
     6541 aaagtgaaat tccgtgcgct gtttctggag ctgtttttcc gccgtacggt gaatttccac
     6601 accgaagagg tgatgctgga caaaattacc ggaaaaacgc cggtggcggc ctatgtctcc
     6661 ccgatcgttg aaggaaaagt gcttcgccat cgcggtggtg aaacccgcgt gttacgtccg
     6721 ggctacgtca agcccaaaca cgaatttaat taccagcagg cggttgagcg ccttcctggt
     6781 gaagatccgg ctcagctgaa cgacccggcc taccgtcgtc tgcgtatcat taccgataac
     6841 ctcaaacagg aagagcacgc cattgtccag gtggaagaaa tgcaggcggt gaatgccgtg
     6901 ctgtatggca aatacaccat ggaaggggat cagtttgata ctgtcgaggt ggatttcggg
     6961 cgctctgaag gaaataacat tgagcaggct gacggtaaaa aatggtctga gcaggaccgt
     7021 gatacgtttg atccgacgca tgatattgac ctctactgcg atcaggccag cggccttgtg
     7081 aatatcgcca ttatggacgg tacggtctgg cgtctgctga atggctttaa gctgttccgc
     7141 gaaaaactgg atacccgtcg cggctcaaat tcacaactcg aaacggcagt gaaagacctg
     7201 ggggcggtgg tgtccttcaa ggggtattac ggcgatctgg ccattgtggt ggcgaaaacg
     7261 tcttatgtgg cagaggacgg taccgaaaaa cgttatctgc cggagggcac actggtcctg
     7321 gggaatacgg cagcagaggg cattcgttgc tatggtgcca ttcaggatgc gcaggcgttg
     7381 tccgaaggtg tggtggcctc ttcccgttat ccgaaacact ggctgactgt gggcgatccg
     7441 gcccgtgaat tcaccatgac gcagtccgct ccgctgatgg tgctgccgga tccggatgag
     7501 tttgtggtgg tacaggtgaa ataatccgtg agcgggggcg aaatgccccc gtgtcttttt
     7561 tcacaggagg ctgagatggc aacaaaagaa gaaaatctga atcgtcttcg tcaactggct
     7621 ggcctgctgg ggcgcgaggc ggatatgtcg gggagtgctg cggatattgc tcaacgtgtg
     7681 tctgagtggg aagaggagct tgctgtttcc ccggagggca ttatgcactc tgatgagagc
     7741 ggggctgatc aaaatcacac agacgatggt gagcagttga acaacacgga tgctccggat
     7801 gatgttaaag ccgtccgggt acggaagtgc ctgcaagtaa tggggtattg cccggagaca
     7861 ggtcgtcccg ttgagctggc gttacggggt atgcgtgttc tggtgccatc atcactggca
     7921 acggccatga tacagcacgg aacggctgaa tatgcgtgat tttcagaatg cctttgatgc
     7981 tgccctcgcc ggggtggaca gtacgatcgt tgaagtgatg gggctctgtg cgcagttcac
     8041 ctcgggggca cagtgtggca gcgaagttca gggggttttt gacgatccgg agtcgctggg
     8101 gtttgccggt ggcggggtcc gtattgaagg aagcagcccg tcattatttg tgcggacgga
     8161 tacggttcgt gccgtgcggc gtggtgacac gctgaccatt aatggtgaga tattctgggt
     8221 ggatcgtgtt tctccggatg acgggggcag ctgttatctc tggctcaacc gtggtcaacc
     8281 acccgcagtt aaccggcgac gataaacgca gggtgaaatt atggcgataa aagggcttga
     8341 tcaggcgatt gacaatctga gccgggttcg taaaaacgcc attccggcgg cttcagcaat
     8401 ggccattaac cgcgtggcca caacggcgat taatcagtct tcatcacagg ttgcccggga
     8461 gacaaaggtt cgccggaaac tggttaagga acggtccaga ctgaaacggg ccacggtcag
     8521 aaatccgaat gccagaatta tcgttaaccg cggtgatctc cctgtgatta agctggggat
     8581 caggatgctg gggcgtcgcc cgaacagcat acttaaagcc ggtcagcatc ggtatcagcg
     8641 ggcatttatt cagcgattaa aaaatggtcg ctggcatgtc atgcagcgtg tggccgggaa
     8701 aaaccgttac cccattgatg tggtgaaaat cccgatggcg gccccactga aacaggcatt
     8761 tgatgagaat gttgaccgta tccggcgtga acgcctgcct aaagaactgg catacgcgct
     8821 gaaacaacaa ctgaggattg cgataaaacg atgaaacaca ctgacattcg tgccgcagtg
     8881 ctggatgcac tcgagcagca tgaacacggg gcgacgctgt ttgatggtcg ccccgttgtt
     8941 tttgacgaag aggattttcc tgcgatcgcg gtttatctga cggatgcaga gtataccggt
     9001 gaagagctgg atgcagatac ctggcgggcc acactgcata ttgaggtgtt tttaccggca
     9061 caggtacctg attcggagct cgatcagtgg atggaaagcc ggatttatcc ggcgatgact
     9121 gcgatccctg cactggcagg actgattacc acgatggtta cgcagggcta tgagtatcgt
     9181 cgtgatgacg atatggcgtt atggagctct gcggatctga cttattccat tacatacgag
     9241 atgtgaggac gatatggcaa caccaaatcc cctggagccg gtaaaaggtg ccggtaccac
     9301 tctgtgggtt tacaacggca agggtgatgc ttatgcaaac ccgttgtcag acgatgactg
     9361 gcagcgactg gctaaggtga aggatctgac gccgggcgag atgacggcag aaccctacga
     9421 tgataactac ctggatgatg aagacgcgga ctggaccgcg accgggcagg ggcagaagtc
     9481 tgcaggagat accagtttta cgctggcctg gaaaccggga gaagatggtc agaaagggct
     9541 tataggctgg tttgaaagcg gggatgtgcg ggcctataaa atccgttttc cgaacggcac
     9601 ggtggatgtg tttcgtggct gggtcagcag tatcggtaag gccgtgacgg cgaaagaagt
     9661 gatcacccgc acggtgaaag tgaccaacgt gggcaaacct tccgtggcgg aagaacgcag
     9721 caaaattacg ccggtcactg caattaaggt gacaccgaca tccggtaccg tggaaaaagg
     9781 aaaaacaacc accctgacgg tttcttttga gccggaaagt gcaaccgaca agacgttcag
     9841 agcggtttcc gccgatccgt caacgggaac cattgctgtg aaagatatgg tgatcactgt
     9901 gacggggatt aaggccggaa aagtgagtat ccccgtgatt tccggtaatg gtcagtttgc
     9961 cacggtagct gaagtcaccg ttactgaagc gggcgctgca gggtaaacgg aggtaataca
    10021 tgtttctgaa aacagaacaa tttgaatata acggtgtgtc tgtcacgctt tcggagctgt
    10081 ctgcgctgca gcgtatcgag catcttgccc tgctgaaacg gcgggcagaa gaggctgaag
    10141 ccagcggcaa cctgcaggtg agtgtggaag atcttgtcag aaccggcgcg tttctggtgg
    10201 cgatgtccct gtggcataac catccacaga gaacgcagtc accgtcaatg aatgaggccg
    10261 tgatgaagat agagcaggaa gtgctcacca cctggcctgc cgatgccatt gcccgggcgg
    10321 aagacgtggt gttgtgcctg tccgggatga tcgaagctgt tcgtccggat actgatatta
    10381 ctgaagtggc gaaaaataac acgctgactg atgatgattt ttctgcggga aagtcttcga
    10441 cggcgagctg aactttgccc tcagactggc gcgtgagatg gggagacccg actggcgcgc
    10501 catgcttgcc gggatgacat ccaccgaata tgccgactgg cgacattttt accgcacgca
    10561 ttattttcag gatacccaac tggatatgca tttttccggg ctgatgtacg ccgtactcag
    10621 cctgtttttt tgcgatccgg atatgcatcc ctcggatttc agtctgctgg ctccccggcg
    10681 agaggaagcg cagacggaga tgccggatga ggaagacatg ctgatgcaga aagcggcagg
    10741 acttgccgga ggcgtccggt tcggtgggga gggagggggc gatatttcac cttctgcaga
    10801 tgtggtggat gtcagcgagg atgatgttgc attaatgatg gcttcagcgg ggatttccgg
    10861 aggtgtgaga tatgtcccag ccggttggtg atcttgttat tgacctgagt ctggatgctg
    10921 tccgtttcga tgagcagatg agccgggtaa ggcgtcattt ttcaggactg gagaatgatg
    10981 ccagaaaaac cgccggtgcc gttgagcaga gtctgaaccg tcaggcgctg gccgcacaaa
    11041 aagccgggat ttccgtcggg cagtataaag cagccatgcg taccctgccc gcacagttta
    11101 cggatatcgc cacgcagctt gccggtggtc agaatccctg gctgatcctg ctgcaacagg
    11161 gcggtcaggt gaaggactcc ttcggcggga tgatccccat gttcaggggg cttgccggtg
    11221 cgatcaccct gccgatggtc ggggtcacct cgctggcggt ggcgacaggt gcgctggcgt
    11281 acgcctggta ccagggggat tccacgcttt cagaatttaa taaaacgctg gtcctttccg
    11341 gcaatcaggc cggactgact gccgatcgta tgctgacgct ctcaagagcc gggcaggcag
    11401 cagggctgac gtttaaccag gcgagagagt cactggcagc cctggtgaat gccggtgtgc
    11461 gtggtggtga acagtttgat gccattaacc agagtgtcgc gcgttttgcg tctgcatccg
    11521 gtgttgaagt ggacaaggtt gcagaggctt tcggaaaact gaccactgac ccgacgtcgg
    11581 gactgatggc gatggcgcgc cagttccgta acgtgacggc agagcagatt gcgtatgttg
    11641 cacaactgca gcgttccgga gacgaggccg gggccttaca ggcggcgaac gatatcgcca
    11701 cgaaaggctt tgatgagcag acccgtcgcc tgaaagaaaa catgggaaca ctggagacct
    11761 gggcggataa aacagggaag gcattcaaat cgatgtggga tgccattctg gatatcggtc
    11821 gtccggaatc ctcagcggat atgctcgcca gtgcgcagaa ggcatttgat gaggcggata
    11881 aaaaatggca gtggtaccag agccggagcc agcgccgcgg taaaacctcc tctttccggg
    11941 ccaaccttca gggcgcatgg gatgaccggg aaaatgcccg tctgggtctg gcagcggcaa
    12001 cgctgcagtc ggatatggaa aaagccggtg aactggcggc aagggacagg gctgagcgtg
    12061 agtcgtcaca gctgaagtat accggagagg cgcagaaggc gtatgagtgc ctgctgacgc
    12121 cactggagaa atataccgcc cggcaggaag agctgaataa ggccctgaaa gacgggaaaa
    12181 tcctgcaggc ggattacaac acgctgatgg cgtcggcaaa aaaggattat gagtcgacgc
    12241 agaaaaagcc gtccggtgtg aaggtgtctg ccggtgagcg ccaggaagac cgggcgcatg
    12301 cagccatgct ggcgcttgaa accgagctca ggacgctgga aaaacacagc ggtgtgaatg
    12361 agaaaatcag ccagcagcgc cgggatttat gggaagcgga aaatcagtat gtggtcctga
    12421 aagaggccgc cacgaaacgg cagttatctg agcaggaaaa atccctgctg gctcatgaga
    12481 aagagacgct ggagtacaaa cgccagctgg ctgagctggg cgacaaggtt gaacaccaga
    12541 agcggctgaa tgagctggca cagcaggcgg cgcggtttga acagcagcag agcgcgaagc
    12601 aggcggcaat cagcgcaaaa gcccgcggcc tcaccgaccg tcaggcgcag cgggagtcgg
    12661 aagagcagcg ccttcgtgac gtgtacggtg ataatccgga tgcgctggcg aaggccacat
    12721 ctgcactgaa gaacacctgg tctgcggagg agcagcttcg tggaagctgg atggcgggtc
    12781 tgaagtccgg ctggggcgag tgggcggaaa gtgcgacgga cagtttttcg caggttaaaa
    12841 gcgcggccac gcagaccttt gacggtattg cacagaatct ggcagcgatg ctgaccggca
    12901 gcgaacagaa ctggcgtggt ttcacccgtt ccgtgctctc catgctgaca gagattttgc
    12961 tgaagcaggc gatggtgggg attgtcggga gtatcggcag cgccattggt ggtgctttcg
    13021 gtggtggcgc atccgcttcc acggggacgg ccattcaggc tgcggcggcg aacttccatt
    13081 tcgcgaccgg gggatttacg ggaaccggtg gcaaatatga gccagcgggg attgtccacc
    13141 gcggggagtt tgtcttcacg aaggaggcaa ccagccggat tggtgtcggg aacctgtacc
    13201 gcctgatgcg gggctatgcg gaaggggggt atgtgggtgc tgccggaagt ccggcgcaga
    13261 tgcggcgggc cgaaggcatt aattttaatc agaacaatca cgtggtgatt cagaacgacg
    13321 gcatcaacgg acaggccggg ccgcagctga tgaaagcggt gtatgagatg gcccgtaaag
    13381 gtgcgcagga tgagctccgg ctgcagttgc gtgatggcgg tctgttatcg gggagcgggc
    13441 gatgaaaacc tttcgctgga aagtgaaacc ggatatggag gtgaactcgc agccatcggt
    13501 gcgtgaagtg cgttttggtg acgggtactc acagcgtatg gcggcagggc tgaatgctga
    13561 cctgaaaaca taccgggtga tgctttccgt gacccgggag gaggcccgtc atctggaagc
    13621 gttcctggca gagcacggtg gctggaaggc atttttgtgg aagccaccct atgcataccg
    13681 gcagataaag gtgacctgtg ccgggtggtc tgcgcgggtc gggatgttgc gcgttgagtt
    13741 cagcgcggag tttaagcagg tggtgaactg atgcaggata ttcacgaaga aagtctgaac
    13801 gagtcggtta aatcagagca gtcaccgcgg gtggtgctct gggaaatcga cctgacggta
    13861 cagggcggtg agcggtattt tttctgcaat gagctgaatg aaaaagggga gccggtcacc
    13921 tggcaggggc ggaagtacca ggcatacccg attgacggca gcggctttga gatgaacggg
    13981 aaagggagca gtgcccgccc gtcactgacg gtgtcgaatc tgttcgggct ggttaccggg
    14041 atggcggagg atttgcagag cctggtgggg gccacggtgg ttcgtcgccg ggtgtatgcg
    14101 cgttttctgg atgcggtgaa ttttgtggca ggcaatccgg aggctgaccc ggagcaggag
    14161 ctgacggacc gctgggtggt ggagcagatg tcgcagctca cagccatgac ggcctcgttt
    14221 gtgctggcga caccgacgga gacggacggt gcgttgtttc ccggccgcat catgctggcg
    14281 aacacctgta tgtggaccta ccgctctgat gagtgtggtt acacgggcgg ggcggtggcg
    14341 gatgagttcg ataaacccac caccgatatc cgtaaggaca gatgcagcaa gtgcatgcgc
    14401 gggtgtgaac tgcgcaggaa tgtcggcaat tttggcggtt tcctttccat taataaactt
    14461 tcgcagtaaa tcccggttta tgacacagac tgaatcagcg attctggcac atgcccggcg
    14521 gtgtgcgcct gcggagtcgt gcggcttcgt gataagcacg ccggaggggg agcggtatat
    14581 cccttgtgtg aatatctctg cagagcctga ggcatatttt cgtattgccc cggaagactg
    14641 gctgcgggca gagatgcagg gggagattgt ggcactggtc cacagtcatc ccggtggtct
    14701 gccctggctg agcgaggctg accggcggct gcagataaaa agcgcactgc cctggtggct
    14761 ggtctgccgg ggtgacattc acaaattccg ctgtgtgcca cacctgacgg gacggcgctt
    14821 tgagcacggg gtgacggact gttacacgct gttccgggat gcttatcatc tggcgggaat
    14881 tgatatgccg gattttcatc gcgaggatga ctggtggcgc aacggccaga acctgtacct
    14941 ggacaatatg gagggaacgg gcttttaccg ggtgcccctg tcctctgcac aggcgggcga
    15001 tattctgctg tgctgctttg gcgcatcggt ggccaaccat gccgccattt actgcggcaa
    15061 cggtgaactg cttcaccatc tgcctgaaca actgagtaaa cgggagaggt attccgaaaa
    15121 atggcaacga cgaacgcatt ctgtctggcg tcaccgccac tggcacgcat ctgccttcac
    15181 ggggatttac aacgatttgg ccgccgcctc agcctgtatg tgaacacggc agcggaagcc
    15241 atccgcgccc tgtcgatgca ggtgccgggc tttcgccgtc agatgaacga aggctggtac
    15301 cagatacgta ttgccggtga ggacacggca ccggaggcgg tgtatgcccg ccttcacgaa
    15361 cagctgggtg agggaacggt catccacatt gtgccgcgac tggccggtgc cggaaagggt
    15421 ggactgcaga ttgtgctggg ggcggcagcc atcgtgggct ctttcttcac tgccggagcc
    15481 acgatggcgt tatggggttc agccctggca gccggtggtt tttctgccac cacgatgctg
    15541 ttttcacttg gagccagcat gattctgggt ggtgtggccc agatgctggc cccgaaggca
    15601 aaaacaccgg attaccgcgc aacggataac ggtaaacaga acacgtactt ttcctcgctg
    15661 gataacatga ttgcccaggg taacccgatg ccggtgcctt acggggaaat gctggttggc
    15721 tcccgccgta tatcccagga catcagtacc cgtgatgaag gcgggggcgg aaaggtcgtg
    15781 gttatcgggc ggcaggggta aaaagaataa aaaaaatccc gcagagttgc ggagctgcgg
    15841 gagattcaga caaatgaaga ttagtgttaa ggagttgttt ttttatgtca cggcaaaaaa
    15901 acactaacgc agcgaaatta tacgcgccac agtcagtttg tgaaaatgtg aagatattca
    15961 gaatttttat tcagtcatga tacaggcatc ctccggggtg cctgttgttt ttt
//
LOCUS       DAZGAN020000109        15958 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZGAN020000109 DAZGAN020000000
VERSION     DAZGAN020000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.109"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..932
                     /locus_tag="ACHKYO_RS19285"
                     /old_locus_tag="pgaptmp_003857"
     CDS             <1..932
                     /locus_tag="ACHKYO_RS19285"
                     /old_locus_tag="pgaptmp_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HGU4430626.1"
                     /translation="VHDTIARVVSCISPAKFHECFINWMRDCHSSNDKDVIAIDGKTL
                     RHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITT
                     DAMGCQKDIVEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAISEKS
                     HGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISS
                     ADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILT
                     NDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            1201..3438
                     /gene="nrfB"
                     /locus_tag="ACHKYO_RS19290"
                     /old_locus_tag="pgaptmp_003858"
     CDS             1201..3438
                     /gene="nrfB"
                     /locus_tag="ACHKYO_RS19290"
                     /old_locus_tag="pgaptmp_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HGU4430627.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3425..6397
                     /gene="nfrA"
                     /locus_tag="ACHKYO_RS19295"
                     /old_locus_tag="pgaptmp_003859"
     CDS             3425..6397
                     /gene="nfrA"
                     /locus_tag="ACHKYO_RS19295"
                     /old_locus_tag="pgaptmp_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HGU4430628.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDASAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6398..7288
                     /gene="ybcH"
                     /locus_tag="ACHKYO_RS19300"
                     /old_locus_tag="pgaptmp_003860"
     CDS             6398..7288
                     /gene="ybcH"
                     /locus_tag="ACHKYO_RS19300"
                     /old_locus_tag="pgaptmp_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HGU4430629.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7471..8232
                     /gene="envY"
                     /locus_tag="ACHKYO_RS19305"
                     /old_locus_tag="pgaptmp_003861"
     CDS             7471..8232
                     /gene="envY"
                     /locus_tag="ACHKYO_RS19305"
                     /old_locus_tag="pgaptmp_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HGU4430630.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNKNTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8745..9698
                     /gene="ompT"
                     /locus_tag="ACHKYO_RS19310"
                     /old_locus_tag="pgaptmp_003862"
     CDS             8745..9698
                     /gene="ompT"
                     /locus_tag="ACHKYO_RS19310"
                     /old_locus_tag="pgaptmp_003862"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HGU4430631.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            9885..11369
                     /locus_tag="ACHKYO_RS19315"
                     /old_locus_tag="pgaptmp_003863"
     CDS             9885..11369
                     /locus_tag="ACHKYO_RS19315"
                     /old_locus_tag="pgaptmp_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4430632.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITH
                     FHELLSKLHHKPLLDSKGENISSGELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(11553..11765)
                     /locus_tag="ACHKYO_RS19320"
                     /old_locus_tag="pgaptmp_003864"
                     /pseudo
     CDS             complement(11553..11765)
                     /locus_tag="ACHKYO_RS19320"
                     /old_locus_tag="pgaptmp_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            11915..12583
                     /locus_tag="ACHKYO_RS19325"
                     /old_locus_tag="pgaptmp_003865"
     CDS             11915..12583
                     /locus_tag="ACHKYO_RS19325"
                     /old_locus_tag="pgaptmp_003865"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4430633.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(12528..12665)
                     /locus_tag="ACHKYO_RS19330"
                     /old_locus_tag="pgaptmp_003866"
     CDS             complement(12528..12665)
                     /locus_tag="ACHKYO_RS19330"
                     /old_locus_tag="pgaptmp_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HGU4430634.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(12638..13222)
                     /locus_tag="ACHKYO_RS19335"
                     /old_locus_tag="pgaptmp_003867"
     CDS             complement(12638..13222)
                     /locus_tag="ACHKYO_RS19335"
                     /old_locus_tag="pgaptmp_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4430635.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTTWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAADLEIATEGE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(13222..>15958)
                     /locus_tag="ACHKYO_RS19340"
                     /old_locus_tag="pgaptmp_003868"
     CDS             complement(13222..>15958)
                     /locus_tag="ACHKYO_RS19340"
                     /old_locus_tag="pgaptmp_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGU4430636.1"
                     /translation="NASASQQSAATSASTATTKASEAATSARDAAASKEAAKSSETSA
                     ASSASSAASSATAAGNSAKAAKTSETNARSSETAAGQSASAAAGSKTAAASSASAAST
                     SAGQASASATAAGKSAESAALSASTATTKAGEAAEQASAAARSASAAKTSETNAKASE
                     TSAESSKTAAASSASSAASSASSASASKDEATRQASAAKGSATTASTKATEAAGSATA
                     AAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSATNSTSETLAAT
                     PKSVKSAYDNAEKRLQKDQNGADIPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAG
                     ATSGKYYPVVVMRSAGSVSELASRVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRY
                     AYGMFWQYQNNERAIHSIMMSNKGDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLV
                     VNDTTYKFGATIPATECIAADVILDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVAR
                     GGNQIRMIGGEYGALWRNDGAKTYLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKL
                     SASLNGNALTATKLQTPRLVSGVEFDGSKDITLTAAHVAAFARRATDTYADADGGVPW
                     NAESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEGWA
                     EVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAVAYPSGVIPDM
                     RGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAH
                     THSVSGSTNSAGAHTHSLANVNTASANSGAGSASTRLSVVHNQNYATSSAGAHTHSLS
                     GTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT         4195 a         3964 c         3802 g         3997 t
ORIGIN      
        1 ctgttcacga tactattgcc agagttgtat cctgtatcag tcctgcaaaa tttcatgagt
       61 gctttattaa ctggatgcgt gactgccatt catcaaatga taaagacgtc attgcaattg
      121 atggaaaaac gctccggcac tcttatgaca agagtcgccg caggggagcg attcatgtca
      181 ttagtgcgtt ctcaacaatg cacagtctgg tcatcggaca gatcaagacg gatgagaaat
      241 ctaatgagat tacagctatc ccagaacttc ttaacatgct ggatattaaa ggaaaaatca
      301 tcacaactga tgcgatgggt tgccagaaag atattgtaga gaagatacaa aaacagggag
      361 gtgattattt attcgcggta aaaggaaacc aggggcggct aaataaagcc tttgaggaaa
      421 aatttccgct gaaagaatta aataatccag agcatgacag ttacgcaatc agtgaaaaga
      481 gtcacggcag agaagaaatc cgtcttcata ttgtttgcga tgtccctgat gaacttattg
      541 atttcacgtt tgaatggaaa ggtctgaaga aattatgcgt ggcagtctcc tttcggtcaa
      601 taatagcaga acaaaagaaa gagcccgaaa tgacggtcag atattatatc agttctgctg
      661 atttaaccgc agagaagttc gccacagcaa tccgaaacca ctggcacgtg gagaataagc
      721 tgcactggcg tctggacgtg gtaatgaatg aagacgactg caaaataaga agaggaaacg
      781 ccgcagaatt attttcaggg atacggcaca tcgctattaa tattttaacg aatgataagg
      841 tattcaaggc agggttaaga cgtaagatgc gaaaagcagc catggacaga aactatctcg
      901 cgtcagtcct tgcggggagc gggctttcgt aatcttgccc tgcctgctca cctcctccag
      961 gcacttccac tacattatga cctgctcggt gccaccgctc cttttcacta tgacgttaac
     1021 ctcccctcct tgacaccatg cttttgtcta cattctacgc aaacggcaac ttttataacc
     1081 agtgtaaaaa taacgtgccg caatagtcat acctattaat ggtaaaaagc tgtcacaatt
     1141 cataaaaaac cttaatatac gccaccctaa acataaccag cgttaatgta aggtttttgt
     1201 gtggactggc ttcttgatgt ttttgcaacc tggctctacg gcttaaaagt aatcgcgata
     1261 acgttagcgg tcatcatgtt catcagcggg ctggacgatt tttttattga tgtcgtctac
     1321 tgggtacgcc gcattaaacg caagttgagt gtttatcgcc gctacccgcg aatgagttac
     1381 cgcgaactgt ataaaccaga tgaaaaaccg ttagcgatta tggttccggc gtggaatgaa
     1441 acgggcgtca tcggcaatat ggccgagctg gcggcgacca cgctcgacta cgaaaactat
     1501 catatctttg ttggcaccta ccccaacgac cccgatactc aacgtgatgt tgacgaagtg
     1561 tgcgctcgct ttccgaacgt gcataaggta gtctgcgcgc gtcctggtcc caccagcaaa
     1621 gccgactgtc tgaacaacgt gctggacgcc atcacccagt ttgaacgtag cgccaatttc
     1681 gcttttgctg gttttattct gcatgacgcc gaagatgtga tttcaccgat ggaattgcgt
     1741 ctgttcaact atctggtcga gcgtaaagat ctgattcaga tcccggtgta tccgttcgaa
     1801 cgcgaatgga cgcacttcac cagcatgact tacattgatg agttttcaga gctgcatggc
     1861 aaagatgttc cggtgcgtga agccctcgcc ggacaagtgc ccagcgcagg cgtcggcacc
     1921 tgtttcagcc gccgcgccgt gaccgctctg ttagctgacg gtgacggtat tgctttcgac
     1981 gtgcagagtc tgactgaaga ttacgacatt ggtttccgcc tgaaagagaa aggtatgacg
     2041 gaaatttttg tccgttttcc ggtggtggac gaagccaaag aacgcgagca gcgcaaattt
     2101 ttacagcacg cacggacgtc aaacatgatc tgcgtgcgcg aatatttccc cgataccttt
     2161 tcgactgcag ttcgacaaaa atctcgctgg atcatcggca ttgttttcca gggctttaaa
     2221 acccacaaat ggacctccag cctgacgctg aactactttc tctggcgcga ccgcaaaggg
     2281 gcaatcagta actttgtcag cttcctcgcg atgctggtga tgctccagct tttgctgttg
     2341 ctggcgtatg aaagtttgtg gcccgatgcc tggcatttcc tttctatttt tagcggcagc
     2401 gcatggttaa tgaccctgct gtggctaaac tttggcttga tggttaaccg catcgtgcag
     2461 cgggtgattt tcgtcactgg ctactacggt ctgacgcagg ggctgttatc tgtcctgcgt
     2521 cttttctggg gcaacctgat taacttcatg gccaactggc gcgcgctaaa acaggtactt
     2581 caacacggcg atccacgtcg cgtcgcgtgg gataaaacaa cgcatgactt ccccagcgtg
     2641 actggcgata cccgctcgtt gcgcccgtta ggccaaattc tgctggaaaa tcaggtcatc
     2701 actgaagaac aactcgatac agcactgcgt aatcgcgtcg aaggtctacg cctgggcggt
     2761 tcaatgctga tgcaggggct gattagcgcc gagcagctgg cacaggcgct ggcagagcaa
     2821 aacggcgtgg cgtgggaatc catcgatgcc tggcagatcc cgtcctcgct gattgccgaa
     2881 atgccggcct ccgtagcgct gcattatgca gtactgccgc tgcgtctgga aaatgatgag
     2941 ttaattgtcg gcagtgaaga tggtattgac ccggtttcgc tggcggccct gacgcgtaaa
     3001 gtcggacgca aagtgcgtta cgtcattgtt ctgcggggac aaattgtcac ggggttacgt
     3061 cactggtatg cacgccgacg cggtcacgat ccgcgggcaa tgttgtacaa tgcggttcag
     3121 catcagtggc tcacggaaca gcagaccggt gaaatctggc ggcaatatgt gccgcatcag
     3181 ttcctgttcg ccgaaatact gaccacgctc ggtcatatta atcgttcagc aattaacgtg
     3241 ttgttattgc gccatgaacg cagttctctg ccgctcggca agtttttggt caccgaaggc
     3301 gttatcagcc aggaaacgtt ggatcgcgtc ctgacaattc aacgcgaatt acaagtttcg
     3361 atgcaatcac tattactcaa agcaggttta aacacagaac aggttgcgca actggagtcc
     3421 gaaaatgaag gagaataacc ttaatcgcgt catcggatgg tctggtttac tgctgacgtc
     3481 tttattgagt accagcgcac tcgcagacaa tatcggcacc agcgcagaag agctggggct
     3541 gagcgattat cgccattttg ttatttatcc ccgtctcgat aaggcgctga aggcacagaa
     3601 aaataacgac gaagcaaccg ccatccgcga atttgaatat atacaccagc aggtgccgga
     3661 taatattccg ctgactttat accttgcgga agcctatcgc cattttggtc atgatgaccg
     3721 ggcgcggctg ttgcttgagg atcaactgaa acgtcaccca ggagatgccc gacttgagcg
     3781 cagtctggcg gctattccgg ttgaagtgaa aagcgttacg actgttgaag aactgcttgc
     3841 ccagcaaaaa gcgtgcgacg ctgcgccgac cctgcgttgt cgcagtgaag tcgggcagaa
     3901 tgccctgcgg ctggcacaat tacctgtcgc cagagcgcaa ctgaacgatg cgacgtttgc
     3961 tgcatcgcca gaaggaaaaa cgctgcgaac cgatctgctg caacgggcaa tctacctgaa
     4021 acaatggtcc caggcagata cgctatacaa tgaagcacgc cagcagaaca cattaagcgc
     4081 ggcagaacgc cgtcagtggt ttgacgtgct tcttgccggg cagctggacg atcggatcct
     4141 ggcactgcaa tcacagggga tcttcaccga tcctcagtca tatattactt acgcgaccgc
     4201 gctggcttat cgtggcgaaa aagcacgcct ccagcattat ctcaatgaaa ataagccgct
     4261 gtttaccacg gacgcacaag agaaaagttg gctctatctg ttatctaaat acagcgccaa
     4321 ccccgttcag gcattggcga attatacggt gcagtttgcc gataaccgcc agtatgttgt
     4381 tggcgcgacg ctaccggtgc tgttaaaaga aggtcagtac gacgcagcgc aaaaactgct
     4441 cgccaccctc cccgccaatg aaatgcttga ggagcgttat gctgtcagcg tggcgacccg
     4501 taacaaggct gaatctctgc gtctggcacg attgctgtat cagcaagaac cggcaaatct
     4561 tacccgcctg gatcaactga cctggcaact gatgcagaac gagcagtcac gcgaagctgc
     4621 cgatttattg ctgcaacgct atcctttcca gggcgatgcg cgtgtcagcc agactttaat
     4681 ggcgcgactg gcgtctctgc tggaaagtca tccttacctg gcaacgccgg cgaaggtggc
     4741 gattttatcg aaacccttac cgctggcgga gcaacgtcag tggcaaagtc agttgccggg
     4801 tattgcagat aattgcccgg caatagttcg cttgctgggc gatatgtcgc cttcctacga
     4861 tgcttccgcc tggaaccgtc tggcaaagtg ttatcgggac acgctacccg gtgtggcgct
     4921 gtatgcatgg cttcaggccg aacaacgaca accgaacgcc tggcaacatc gtgcggtagc
     4981 ctatcaggcg tatcaggttg aggactacgc caccgcactg gcggcctggc agaaaatcag
     5041 tcttcacgac atgagcaatg aggatctgct tgctgctgcc aataccgccc aggcggcagg
     5101 aaatggtgca gctcgcgatc gctggctaca acaggcagaa caacgtggac tgggaaacaa
     5161 tgccctctac tggtggctgc atgcgcaacg ttacattcct ggtcagccgg aactcgcact
     5221 gagcgatctc acgcgctcaa tcaatattgc cccttctgcc aacgcttacg ttgcgcgggc
     5281 gacaatttat cgccaacgtc ataatgtccc ggcggcggtg agtgatttgc gcgccgcgct
     5341 ggaactggaa ccgaataata gcaacatcca ggcagcgctt ggttacgcct tgtgggatag
     5401 cggtgatatc gcacagtcgc gggaaatgct cgaacaggcg cataaagggc ttccggacga
     5461 tccggcactg atccgacaac tggcctacgt gaaccagcgt ctggatgaca tgcctgcgac
     5521 gcagcactac gcccggctgg tgattgatga cattgataat caggcgctga taaccccact
     5581 gaccccagag caaaatcagc aacgcttcaa tttccgccgt ttgcatgagg aggtcggtcg
     5641 ccgctggacg ttcagtttcg attcttctat cggcttgcgt tccggggcaa tgagcaccgc
     5701 taacaataat gtcggcggcg cagcgccagg gaaaagctat cgtagctacg ggcaactgga
     5761 agccgagtac cgcatcggac gcaatatgct gctggaaggc gacctgctct cagtttatag
     5821 ccgcgtcttt gccgataccg gagaaaacgg ggtgatgatg ccggtgaaaa atccgatgtc
     5881 cggcaccggt ctgcgctgga agccgctgcg cgatcagatc tttttcctcg ccgtcgaaca
     5941 gcagttgccg ctgaacggcc aaaatggcgc atccgatacc atgctgcgcg ccagcgcctc
     6001 attctttaat ggcggcaaat acagcgacga atggcacccg aacggttcag gctggtttgc
     6061 ccaaaacctg tacctcgatg cggcgcaata tatccgccag gatattcagg cgtggacggc
     6121 agattatcgc gtcagctggc atcagaaggt agctaacgga cagactattg agccttacgc
     6181 tcacgttcag gacaacggct atcgtgataa aggcactcag ggcgcgcagc ttggcggagt
     6241 cggggtccgc tggaatatct ggaccggcga gacgcactac gacgcctggc cgcacaaagt
     6301 cagtctcggc gtcgagtatc aacatacctt taaggcgatt aatcaacgta acggagagcg
     6361 caacaacgcg tttctcacca ttggagtgca ctggtaaatg cgtaagttca ttttcgtatt
     6421 gctgacactg cttttggtca gccctttttc ctttgcgatg aaaggtatta tctggcaacc
     6481 acaaaaccga gatagtcagg ttaccgatac ccagtggcag gggctgatga gtcagttacg
     6541 tttgcaaggc ttcgataccc tggttttgca atggacccgt tacggcgatg cattcaccca
     6601 gccagaacag cgcgcgttat tgtttaagcg tgccgcagct gcgcaacagg ctggcctgaa
     6661 gctgattgtc gggctgaacg ccgatccgga attttttatg caccagaaac agtcgtccgc
     6721 agcgctggaa agctatctta atcgcctgct ggctgccgat ctccagcaag ccagattatg
     6781 gagcgccgcg cctggcgtaa cgccggatgg ctggtacatc agcgcggaaa ttgacgacct
     6841 gaactggcgc agcgaagctg cccgtcagcc tttgctaaca tggttaaaca acgcgcagcg
     6901 gctgattagc gatgtttcag caaaaccggt ttatatcagt agttttttcg ccggaaacat
     6961 gtcgcccgat ggctatcgcc aactgctgga acaagttaaa gcaaccggcg ttaatgtctg
     7021 ggtacaggat ggcagcggcg tggataaact taccgctgaa cagcgtgaac gttatctaca
     7081 ggccagcgcc gattgccaaa gtcccgcccc tgccagcggc attgtttatg aactgtttgt
     7141 cgccggcaaa ggcaaaacct ttacagcgaa accgaaaccg gatgcagaga tcgcctcgct
     7201 gttagcgaaa cgttcctctt gcggtaaaga cactctctat ttctctctgc gctatttgcc
     7261 cgtcgcgcac ggcattctcg agtattaaat ctcctccagg taagtcgggt acgacctggc
     7321 ttacctcttt cgctctcgtc agaattccat caagattaat tgctaaaaag cgtgctaaag
     7381 aaagaagatc gttatcggcg aacgggccga tcactcatgc aggaagcata gatcctttga
     7441 gcggagtttc tgatatctga ggagtgcgaa atgcaattga gcagcagtga accttgcgtg
     7501 gtgatcctga ccgaaaaaga ggtagaggta agcgtcaata accatgctac gtttaccctt
     7561 ccgaaaaact acctggccgc cttcgcgtgc aacaataacg tcattgaact ctcaacgtta
     7621 aatcacgtat taatccccca catcaaccgt aacatcatca acgattatct gttgttttta
     7681 aataagaact taacctgtgt aaagccctgg tcgcggctgg caaccccggt tatcgcttgt
     7741 catagccgta caccggaagt gttccggcta gccgccaacc acagcaagca gcaacccagc
     7801 aaaccctgcg aagcggagtt gacgcgcgca ttacttttta ccgtattgtc taactttctt
     7861 gagcaatcgc ggtttatagc cctactgatg tatatcttac gcagcagcgt ccgcgacagc
     7921 gtctgccgca ttattcaaag cgatattcag cattactgga atctgcgaat tgtcgccagt
     7981 tcgctatgtt taagccccag cctactcaaa aagaaattaa aaaacaaaaa taccagctat
     8041 agccagattg tcacagagtg tcgtatgcgt tacgccgtac agatgttatt gatggataac
     8101 aaaaatatca ctcaggtggc gcaattatgt ggctatagca gcacgtcgta ctttatctct
     8161 gtttttaagg cgttttacgg cctgacaccg ttgaattatc tcgccaaaca gcgacaaaaa
     8221 gtgatgtggt gaagggcaaa gcggaaacgg ataagacggg cataaatgaa gaagaaatgg
     8281 cgcgccctgc aggattcgaa cctgcggccc acgacttaga agttcctaga acgacatttt
     8341 aagtcaacaa cttaccgcgc catctctgcg ctcacacgtc ccactacctc aaaacatgta
     8401 aagccttgca agccattgtg aggccttatg tgtctcagtt ttgtccctct tttttgtacc
     8461 aaaaaacata gcaattgagg ataaacctca tgctattttc gcttatatgc ctctaaaggc
     8521 atggcgctta aatagataaa aacatcacaa aagcataaaa aaccacacag taaaaccgca
     8581 acataaaaca ataacagata attaaaccaa aaacagatag cgcattgtga taatcattca
     8641 atactaaaca aaatataaac agtggagcaa tatgtaattg actcattaag ttagatataa
     8701 aaaatacata ttcaatcatt aaaacgattg aatggagaac ttttatgcgg gcgaaacttc
     8761 tgggaatagt cctgacaacc cctattgcga tcagctcttt tgcttctacc gagactttat
     8821 cgtttactcc tgacaacata aatgcggaca ttagtcttgg aactctgagc ggaaaaacaa
     8881 aagagcgtgt ttatctagcc gaagaaggag gccgaaaagt cagtcaactc gactggaaat
     8941 tcaataacgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc cagatatcta
     9001 tcggggctgc tggctggaca actctcggca gccgaggtgg caatatggtc gatcaggact
     9061 ggatggattc cagtaacccc ggaacctgga cggatgaaag tagacaccct gatacacaac
     9121 tcaattatgc caacgaattt gatctgaata tcaaaggctg gctcctcaac gaacccaatt
     9181 accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca gccagaggtg
     9241 gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc ttcccgaatg
     9301 gagaaagagc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc ttgactggaa
     9361 gttatcgtta tgaagatttt gagctaggtg gcacatttaa atacagcggc tgggtggaag
     9421 catctgataa cgatgagcac tatgacccag gaaaaagaat cacttatcgc agtaaagtca
     9481 aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtaaca cctaacgcaa
     9541 aagtttatgt tgaaggcgca tggaatcggg ttacgaataa aaaaggtaat acttcacttt
     9601 atgatcacaa tgataacact tcagactaca gcaaaaatgg tgcaggcata gaaaactata
     9661 acttcatcac tactgctggt cttaagtaca ccttttaacg aagttaacca gattttctcc
     9721 ccggtggtat tgtataaccc cggggaattt gtttacagtt acaagtagac aatatccact
     9781 aattttgtaa gcatttaacc tatgtcataa cgtgtaatct cttaccgcaa cacaagacct
     9841 caatatattc aaattttatc tttattccga tattcagaga cacaatgcga aaaataatta
     9901 ctcatttcaa agttgtttta acgttacttc taccagtaac cgtatctgcc cagcagatac
     9961 agtggcaatc ctgtatggcc agtcaattca accactggtt tggtgaggaa aaaccgtctc
    10021 ctgacttact atgtggttat ttgtctgttc cattaaaata tacagacaca ggcggagatg
    10081 cttcttatga aaaaaaatca caagtcaaac tagcgttgac aaaattgccg gcaaaaagca
    10141 agcataaagg aagtatcctg ataataagtg gtggtcccgg gttaccaggc ataaatcctt
    10201 atattaactt tgactggcca gtcacaaatc ttcgtgagtc atgggatatt attggatttg
    10261 atcctcgagg cgtcggacag tccactccga caataaactg ccggcaatca gatacagaga
    10321 ctcaggaaaa cataaccgaa aagcaacaag tattaaataa aattaatgcc tgtatccata
    10381 ataccggagc cgaagtcatt cgccatatcg gctctaacga ggctgtatac gatattgatc
    10441 gtattaggca agccttgggg gataaacaac tgacagccgt ggcgtattcg tatggaactc
    10501 aaattgcagc cttatatgca gaacgttttc cctacaacgt aagatctatc gttcttgatg
    10561 gagtcgtcga tatcgatgac ctggaggaca acttcacatg gcaactcaaa caggcacaga
    10621 gttatcagga aacgtttgat cgctttgcat cctggtgtgc gcgtacaaaa agttgcccgc
    10681 tttcttcaga cagagataag gcaataactc acttccatga gctattatca aaattacatc
    10741 acaaaccttt attagacagt aagggagaaa atatatcttc aggtgaactc atatcattaa
    10801 caacagacct tctgctatgg cgttcatcat ggccaaccct tgcaactgcc atacgccagt
    10861 tctctcaggg gattgtcagt aatgaaattg aaactgcgct cagtgctccg atagcctcag
    10921 aagagtcaag cgatgcttcg ggggtaatcc tctgtgtaga tcagggggat gagcaattaa
    10981 caccagaaga gcgaaaatcc cgaaaagacg ctcttgcgaa tgccttcccg gctattaact
    11041 ttgacaatgg acgttccgat tcacctgatt tttgtgaatt atggccaata catagcgacc
    11101 tgaacaaaac tcgcctgaaa aatactgttc tgccctctgg tttactgttt gtagcacaca
    11161 aatacgaccc aacaacgccc tggattaatg cccgtaagat ggcagagaaa ttttccagcc
    11221 cgttactaac aataaatggt gatgggcata cattagctct caccggagtt aatttatgtg
    11281 tagataaagc agttgtacat cacctgatca ctccacaaaa aatagaaaat atatactgcc
    11341 caggaaattc tgaagcagaa atacaataat ttcaaaatat tccgctattt gctcccaatg
    11401 caaaacatat attgcattgg gagcatattc atatttttgt tattcttgta tgaaatcgtc
    11461 tcatgtacac ggtccttttt tcgccgaaaa ctgatattac tttaatcaaa agttgacgtg
    11521 ccagattcga agtaaattta taaaatatca aatcaatata cattttgcac cttcgagata
    11581 gtaatggctg ttactgtaac atgagttaca aacagcttca tacccagagc tactgatgta
    11641 tcaacacgat tcaacaatac ccgatattta ttccatgcct ccagcaacga tctttcttcc
    11701 tccgttgcga tttccagatc tacagcatcc tgcagtggcg caatatactc actgaattcc
    11761 tggatgtaga actgtgtggt gacggtcttc cagctattcg gctcctgctg tatcgaagca
    11821 taccaggcta tttcaatatc gctatgctgc ggcagcattt aaccccttgt aattcatcgc
    11881 cataattgat ttaattcaca aataaaacta taacatggtg aaattaatga aaaaaaacac
    11941 agatgatggg gctaaaattt acacaccact taccctaaag ctttatgact ggtgggtttt
    12001 gggagtatca aatcggcttg catggggatg tcctacaaag gaacaccttc ttccacactt
    12061 tctggaacat ttaggtaaca accatctgga tattggtgtt ggaactgggt tttaccttac
    12121 tcacgtacct gagagtagtc tgatatcttt aatggatttg aacgaagcta gcctgaacgc
    12181 ggcatctaca agggctgggg aatcaaaaat taaacataaa attagccatg atgtttttga
    12241 accttatccc gcggcgttac atggtcaatt tgattccatt tccatgtttt accttcttca
    12301 ctgcctgcct ggaaatatat ctacaaaaag ctgtgtaata cgcaatgcgg cgcaggcctt
    12361 aactgacgat ggaactctat acggagccac aattcttggc gatggagttg tgcacaatag
    12421 cttcggtcaa aaactgatgc gcatttacaa tcagaaaggc atcttttcaa acacaaaaga
    12481 ttccgaagaa ggcttaacac atatactctc agagcatttc gagaatgtta aaaccaaggt
    12541 tcaaggtact gtagtaatgt tttccgcttc agggaaaaaa tagcatccaa ccgcagcacg
    12601 ttcttgctta agacgtgctg cggcataatc ccaatgatta ctccctgaca gggttcgtag
    12661 gccactcaat atcaggtgca gttgatgtat caacacggtt cagcaacacc cgatactttt
    12721 tccaggcttc cagcaacgag gtttctccct ccgttgcgat ttccagatct gcagcatcct
    12781 gaagtggcgc aatatgctca ctggctacct gcatcaggct gttttttgtt tcttccgcct
    12841 cccggatccg gaacagtttt tctgcttctg catctttcac ccaggttgtg ccgttccact
    12901 tctgaaactc cccttccggg gataaccagg tgacattttc cggtaatgag ccgagttcag
    12961 aaataaataa cgcgtcgccg gaagccacgt catagacggt tttaccccga tggtcttcaa
    13021 cgagatgcca cgatgcctca tcactgttga aaacagccac aaagccagcc ggaatatctg
    13081 gcggtgcaat atcggtactg tttgctggca gacctgtatg aggcggaata tatgcgtcac
    13141 cttcaccaat aaattcatta gttccggcca gcagattata aatttttatg gtccgtggtt
    13201 gttcactcat tctgaatgcc attatgcaag cctcacaata tagttaaatg cgatgttttt
    13261 gacggtgttt tccgcgttac cagcagcgtt aacggtgatg gtgtgtccat gtgagccaat
    13321 cgcaaccgag tgcgtatgag caccaatacc gacagtatgt gcgtgtgcac ctgcgcttgc
    13381 agcagtgccg gacagtgagt gggtatgtgc gccagcagat gatgttgcat agttttgatt
    13441 atgcacaaca gacaatcttg ttgatgcact accagcaccg gagttagcac tagccgtgtt
    13501 cacgttggct agtgagtgtg tgtgtgctcc agccgagttt gtagagccgc tcacactgtg
    13561 tgtatgtgcc ccggtgttat tcgtagattt agtgccgtaa tcaaacgacg atgtggtttt
    13621 cgtacccaaa tccgtactgg atgcgctggc gctgtgggtg tgcgatttaa tgccgtcctg
    13681 ttcctgagac aatacggcac gaccactggc gggcttgccc ttaatcgtcc agccacgcat
    13741 atcagggatc acgcctgacg gataagcaac tgcaagtttc gggtatgcag atttgtcaaa
    13801 agtctgcccc tgcatcaggg cataaccaga cggaacggta tctgatggcc acggaattgg
    13861 tgcgccaact gggtagcttt ctggtggaag atttttcgag gtataaactt ctgcccagcc
    13921 ttcctcaaaa ccatagccat ctcttgagga acggtagaac agacctccat ttctgtaatg
    13981 cgccttcatc tgcagggtcc ggcaacttcc gactccggta tagaagttaa ccagaatata
    14041 gctgtcgcca gagcgggtga cattgtaagc gcctgattcg gcattccagg gaacgccacc
    14101 atccgcatcg gcatatgtat ccgttgccct tctggcaaaa gcagccacat gcgcggcggt
    14161 taaagtaata tcttttgaac catcaaactc aaccccagaa accagtcttg gcgtttgcag
    14221 ctttgttgct gtcaatgcgt taccgttcag acttgcggac agtttggttc caataaccag
    14281 ttcgccggtt gcgttatcaa tagcaaacgg tcttaatgta ttccagccac cataaacatc
    14341 accttgattg gtaagcagca ggtaagtttt agcgccatca ttacgccata atgccccata
    14401 ctccccacct atcattcgaa tctgattacc accacgcgct acaatttcgt ctgtggcaaa
    14461 aagttttttg cacgacaagt tatcgttaac gattaacgaa tgggactcat aaaaaccacg
    14521 cccactctta aaatcaagga taacgtccgc cgcgatacat tcagtcgctg gaattgttgc
    14581 cccaaactta taggtcgtat cattaacaac gagatcagca ccaggtgcgg atattgacag
    14641 gccatcttcg ataaacgcaa aaacagggaa agcagcgcca tcaacataga acacagagcg
    14701 caaatcatcg cccttattac tcatcattat tgagtggatg gctcgttcat tgttttgata
    14761 ttgccagaac attccataag cataacgccc cctgtcagtc cagccaccag gcataacaaa
    14821 tccgttaaac tcgcagttat tcatcggatc gcctgcggtt cgcgttgccg tggtgataat
    14881 gacccttgat gccagttcgc ttactgagcc agcagaacgc ataacaacaa cagggtaata
    14941 ttttccagat gttgcacctg caggagcgtt aacccgcaca taacgcatac cacgcttatc
    15001 agcaaagtct gttttactga ccgcgttaat gttgttcagg aagcgtccct tatcgggtat
    15061 atcagcgccg ttctggtctt tctgcagacg tttctctgca ttgtcatagg ctgattttac
    15121 tgactttggc gttgccgcca gcgtttcaga cgtactgttg gtcgcactgc tgagctgtac
    15181 tatccccttt ttcgtcgtac ttgcatcctc aagcgccacg gcggatgcaa tatcctctgc
    15241 ccgtttagct gctgtctcgg cgcgcgttgc cgcggattcc gccgtacttt tgctctgtgc
    15301 tgccgctgtc gcactgccag cagcctctgt cgccttcgtg gatgccgtcg tggcgctgcc
    15361 cttcgctgct gacgcctgtc tggtcgcctc atcttttgaa gcagacgcag atgatgccga
    15421 tgacgccgcc gaactggcag acgatgccgc tgccgttttt gaggattctg cgctggtttc
    15481 cgacgctttc gcgttcgttt cggatgtctt cgctgcggat gcagacctcg ctgctgcgct
    15541 ggcctgttca gcggcttcgc cagccttcgt tgtggctgtt gaagcggaca atgcagcgct
    15601 ttctgccgat tttccggcgg cggtggcact ggctgaggcc tgcccggcac ttgttgacgc
    15661 tgcactggca gacgacgcag ccgctgtttt tgagcctgcc gcagctgagg cgctctgtcc
    15721 cgctgccgtt tcagaagacc tggcgttcgt ctcggacgtt tttgccgcct tcgcggaatt
    15781 tcctgccgcc gttgccgagg aagctgcact actggcgctc gaggctgcgc tcgtttctga
    15841 tgattttgcc gcctcttttg aggccgccgc atcccgggcc gaggtcgcag cttctgatgc
    15901 cttcgtggtc gcggtggatg cagatgtggc tgctgattgt tgtgacgctg aagcattc
//
LOCUS       DAZGAN020000110        15816 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZGAN020000110 DAZGAN020000000
VERSION     DAZGAN020000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.110"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..564
                     /gene="espX1"
                     /locus_tag="ACHKYO_RS19345"
                     /old_locus_tag="pgaptmp_003869"
     CDS             <1..564
                     /gene="espX1"
                     /locus_tag="ACHKYO_RS19345"
                     /old_locus_tag="pgaptmp_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HGU4430637.1"
                     /translation="MYELNDQKIQYLTAPNINIHKMSLSNITIEKSNLSYGYYFSCVL
                     SNISCFESDLSNTIFSNGEINNLFIKKSNIFGASFTNTRIKNLLCEDIMPGRWTTQLV
                     NKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELKKFMHDYD
                     KTSPEMRKYLQSINESMFLMKNIAHQN"
     gene            790..2142
                     /locus_tag="ACHKYO_RS19350"
                     /old_locus_tag="pgaptmp_003870"
                     /pseudo
     CDS             790..2142
                     /locus_tag="ACHKYO_RS19350"
                     /old_locus_tag="pgaptmp_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2155..3114
                     /locus_tag="ACHKYO_RS19355"
                     /old_locus_tag="pgaptmp_003871"
     CDS             2155..3114
                     /locus_tag="ACHKYO_RS19355"
                     /old_locus_tag="pgaptmp_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGU4430638.1"
                     /translation="MKWLLLITLSLYSFIVQSTPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFEYDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(3152..4051)
                     /gene="nhaR"
                     /locus_tag="ACHKYO_RS19360"
                     /old_locus_tag="pgaptmp_003872"
     CDS             complement(3152..4051)
                     /gene="nhaR"
                     /locus_tag="ACHKYO_RS19360"
                     /old_locus_tag="pgaptmp_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HGU4430639.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(4117..5283)
                     /gene="nhaA"
                     /locus_tag="ACHKYO_RS19365"
                     /old_locus_tag="pgaptmp_003873"
     CDS             complement(4117..5283)
                     /gene="nhaA"
                     /locus_tag="ACHKYO_RS19365"
                     /old_locus_tag="pgaptmp_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HGU4430640.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGNVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5869..6021
                     /gene="mokC"
                     /locus_tag="ACHKYO_RS19370"
                     /old_locus_tag="pgaptmp_003874"
     CDS             5869..6021
                     /gene="mokC"
                     /locus_tag="ACHKYO_RS19370"
                     /old_locus_tag="pgaptmp_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HGU4430641.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(6125..7255)
                     /gene="dnaJ"
                     /locus_tag="ACHKYO_RS19375"
                     /old_locus_tag="pgaptmp_003875"
     CDS             complement(6125..7255)
                     /gene="dnaJ"
                     /locus_tag="ACHKYO_RS19375"
                     /old_locus_tag="pgaptmp_003875"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGU4430642.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(7344..9260)
                     /gene="dnaK"
                     /locus_tag="ACHKYO_RS19380"
                     /old_locus_tag="pgaptmp_003876"
     CDS             complement(7344..9260)
                     /gene="dnaK"
                     /locus_tag="ACHKYO_RS19380"
                     /old_locus_tag="pgaptmp_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGU4430643.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            9637..10041
                     /gene="yaaI"
                     /locus_tag="ACHKYO_RS19385"
                     /old_locus_tag="pgaptmp_003877"
     CDS             9637..10041
                     /gene="yaaI"
                     /locus_tag="ACHKYO_RS19385"
                     /old_locus_tag="pgaptmp_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HGU4430644.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            10067..10780
                     /gene="msyB"
                     /locus_tag="ACHKYO_RS19390"
                     /old_locus_tag="pgaptmp_003878"
     CDS             10067..10780
                     /gene="msyB"
                     /locus_tag="ACHKYO_RS19390"
                     /old_locus_tag="pgaptmp_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU4430645.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            10929..11495
                     /gene="satP"
                     /locus_tag="ACHKYO_RS19395"
                     /old_locus_tag="pgaptmp_003879"
     CDS             10929..11495
                     /gene="satP"
                     /locus_tag="ACHKYO_RS19395"
                     /old_locus_tag="pgaptmp_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HGU4430646.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(11530..12117)
                     /gene="mog"
                     /locus_tag="ACHKYO_RS19400"
                     /old_locus_tag="pgaptmp_003880"
     CDS             complement(11530..12117)
                     /gene="mog"
                     /locus_tag="ACHKYO_RS19400"
                     /old_locus_tag="pgaptmp_003880"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HGU4430647.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(12232..13185)
                     /gene="tal"
                     /locus_tag="ACHKYO_RS19405"
                     /old_locus_tag="pgaptmp_003881"
     CDS             complement(12232..13185)
                     /gene="tal"
                     /locus_tag="ACHKYO_RS19405"
                     /old_locus_tag="pgaptmp_003881"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU4430648.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            13464..14894
                     /gene="yaaJ"
                     /locus_tag="ACHKYO_RS19410"
                     /old_locus_tag="pgaptmp_003882"
     CDS             13464..14894
                     /gene="yaaJ"
                     /locus_tag="ACHKYO_RS19410"
                     /old_locus_tag="pgaptmp_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGU4430649.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            14964..15740
                     /gene="yaaA"
                     /locus_tag="ACHKYO_RS19415"
                     /old_locus_tag="pgaptmp_003883"
     CDS             14964..15740
                     /gene="yaaA"
                     /locus_tag="ACHKYO_RS19415"
                     /old_locus_tag="pgaptmp_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGU4430650.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
BASE COUNT         4127 a         3856 c         3664 g         4169 t
ORIGIN      
        1 atgtatgaac ttaacgatca aaagatccag tatttgactg cccctaatat caatatacat
       61 aaaatgtcgc tatcaaatat aacaatagaa aagagcaacc tttcatatgg gtattatttt
      121 agttgtgtat tatccaatat atcatgtttt gaaagcgact tatcaaacac catcttttca
      181 aatggagaaa ttaataatct cttcattaaa aaaagcaaca tatttggagc atcattcact
      241 aataccagaa taaaaaattt gctctgtgaa gatattatgc ctggtcgatg gaccactcaa
      301 cttgtcaata agcacttagg ttacagatac acaggagtat tcaaaaccct ggcaagtatt
      361 gatgacaaac cgtcccgttt cgaaattttg ataccattgg tacaaacttt agttcgcgac
      421 aatgtaaagc taaataatga tgtgtataaa gaactgaaaa agtttatgca cgattacgac
      481 aaaacatccc cggaaatgag aaaatacctt cagagcatca acgagtctat gttccttatg
      541 aaaaacatag ctcatcaaaa ttaaaaaatc tcaaaaaaga agtaatttca gaatgaaatt
      601 aactgtgatg attattaatt cactacagag gaataaaaaa taatctgaag catactctga
      661 aacatcctac tctctcactc taataatggc accttgctct tcttataaag aagggaataa
      721 ttaagaatat taaactttca tcaacttcac atcaagaaat taaaaaattt aaacattaat
      781 ctaaataata acaacctgat cacataatcg ggtacgattt tatccgataa ctataatgct
      841 ataactctgg gaaatggttg gaatacaccg ctgggtgcca tctcttttta cgctgcccga
      901 tcgagtagta agttaaataa tgataccaga cacgaaggta cgagctatca ggtcgcctat
      961 aataaatatc tagtacaaac agccactcga tttagcgtgg ccgcctggcg ttatgcttcg
     1021 caggattaca ggacattcag cgaccatctt tacgaaaatg ataaacacta tcatcagagt
     1081 gattataatg atttttatga tattggcaga aaaaatagcc tttctgccaa tattatgcaa
     1141 cctttatcca ataatctggg aaatgtatca ttaagtgcgc tttggcggaa ttactggggg
     1201 cgaagtggaa atgctaaaga ttaccaattc agttactcca ataactggca acacattagt
     1261 tatactttct ctgccagcca atcttatgat gaaaataata aagaagagga gcgttttaat
     1321 ctgtttatct ccatttcttt ctactggggg gatgatattg ccaaaacacg tcaccaaatt
     1381 aacttatcga attcgacctc attttccaaa gatggctatt cctccaacaa tactggaatt
     1441 actggcatag ccggtgaaca tgatcagtta aattatggta tatatgttaa tcagcaacaa
     1501 caaaataatg atacctcgct tggtacgaat ttaagctgga gaactcccat cgccataata
     1561 gatggcagct atagtcattc taaaaacgcc tggcaaagtg gtggaagtat tagtagtgga
     1621 ttagttgtct ggtccggtgg tattaatatc actaaccagt tatccgatac atttgcaatt
     1681 ctggatgcgc ctggattaga aggtgcacat attaatggac aaaaatacaa tcgaacaaac
     1741 agcaaaggtc aggttgttta cgacctgata atacctcatc gtgaaaacca tctggtactt
     1801 gatatagcaa acagtgaaag tgaaacagaa ttgcagggca atcgtcaaat tattgcacct
     1861 taccgtggag cagtttctta tgtgcagttt acaactgacc aacgtaagcc ctggtatata
     1921 caggcactgc gtcccgatgg ttcgccatta acctttggct atgacgtact ggatctccag
     1981 gaaaataata ttggagtcgt tggccagggt agtcgccttt ttattcgcgt agatgaaatt
     2041 cccactggaa taaaagttgc tctcaatgat gaacagaact tattctgtac tattactttt
     2101 caacacgtta tcgatgaaaa caaaacatat atatgccagt aaaaggagtt tactatgaag
     2161 tggcttttat taatcacctt gagtctatat tcttttattg tgcaaagtac tccctgtgca
     2221 ttgaccaatg tcggtgaaca acgtggaaca tatatactca agccactgag tatgaaaggt
     2281 aatctaaccg ccgaaaaaat tgaaaacacc ggaaatatca ttgagtgtac agacttgcaa
     2341 gagacaacca acgccattaa agttcatttt ttaacacaga acgctttaag gtttaaagtc
     2401 ggaagtcgtc attatattat taatttcccc tttgaatatg acggtaaaaa aatagagctt
     2461 ccagggaagt catcttataa tttagaagaa atgctatctg cgataaattt caccattcca
     2521 tattctatcg catctgtatc agatagttct aacaccgcag acatttttcc aggaactcct
     2581 tttcctttgt tgttcgaaat tgaagtgagt tcatgtacag ggaataatac taattgtaat
     2641 agcgtccgct ttaattataa tttaaatact attttacagg taaatttaat gacctgtggt
     2701 tttgaagatc aatccatcga cactggacga tttgtactgt ctcgtatcaa taatgaaaat
     2761 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
     2821 gaacccagtg atgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
     2881 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaagtagg ctttcaactc
     2941 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
     3001 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaataat
     3061 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg ttatgtacaa ctaatctatc
     3121 tcctccactt taactttggg ggagctgccg attaacgcgc cgctggacta aaaagcgcag
     3181 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
     3241 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
     3301 cataaaagtc atatgcataa agcgttgggg caacgaagat tgcattgtgc attgcaccaa
     3361 aagctttcat caacgcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
     3421 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
     3481 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
     3541 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
     3601 gacagtcgga aatgatcata tccagtttat gctggcttaa ttgctccagt agcatttcgt
     3661 gggtggattc gaagcagcga agatgaatcg gctcgccttc taccactgca gcattcagca
     3721 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     3781 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     3841 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     3901 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     3961 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     4021 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     4081 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     4141 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     4201 cgcccagtta atcagttctg gatctacgtt accaaaggcc aggctggcaa taaagataga
     4261 catagtaaaa ccgataccgc acaggatccc caccgccata atttgctgat aagtcgttcc
     4321 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     4381 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
     4441 cagcgtgaca ccttgcagtg aaacgccagc attagcaaat gcaaacagcg gcaaaatcag
     4501 atacgccacc cacgggtgta acacatgctc cagtcgcttc gctggagaac gcccatgctt
     4561 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agggttgcgt gaacccccga
     4621 tttcaacacc gcagtccaca acactacgcc aaccagaata tagacgcccg tgcggcgtac
     4681 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagagaggc
     4741 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     4801 aatagccaga gccatcaaaa agatcttcag cgctaacgga acacgacttc ccaacagcgc
     4861 cagcacacca agtgcaaagg caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     4921 aatcggatcg gcatagttaa aggccagata gagtaatgcc ggaacaatca tcccaccaat
     4981 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     5041 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcgt catttatcca
     5101 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     5161 aaagtcgtga taccatccac tggttgcgcc gctattggcc ataatcatcg ccaggatagc
     5221 ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaatcgat gcagatgttt
     5281 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacaaatc
     5341 atcgttaaaa tcagattata gtgtaggaat aactcgtttt ttacgatcca ttgaagaatt
     5401 tgcgccatca gcggtgagat gagtttttga cggctgtaag cgctcacgag tgaccctaag
     5461 cccagataat catagcttaa ggtgaggatt tctgagagat taattaggaa aagtaataat
     5521 taatttgttg tcagtcggcg atgtttgtta ggccaatatt tcaaaaataa tccagcttgt
     5581 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     5641 cgccataaaa atgcgaggcg tcttcaggat tagaatcgat aaaacagaat gggttaaaga
     5701 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
     5761 gatagagaaa agccccgagt gatattttac catcaacccg aggcccccta tatgctgaac
     5821 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     5881 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta
     5941 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     6001 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     6061 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     6121 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     6181 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     6241 gtttctgctt ctcgttcagg cctaccggtg tttcgacgac aacgcggcac agtaaatcac
     6301 cctgtgcgcc accgcggaca gacttgacgc ctttaccgcg catacggaac agcttaccag
     6361 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     6421 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     6481 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     6541 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     6601 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     6661 tgttgcacgg atctttgatc agcgtaccgc gaccctgaca gtgtggacag gtctgctgca
     6721 cggcaaagaa accctgacgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     6781 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     6841 gaatgcggat ctctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     6901 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     6961 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaaccg ccgccgccca
     7021 taccaccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     7081 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     7141 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ttgatttcac
     7201 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     7261 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     7321 ataattaccc gtttataggg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     7381 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     7441 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ctcctgcatt
     7501 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagtgca
     7561 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     7621 cgggtgctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     7681 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     7741 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     7801 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     7861 cgcggtgccg ggttgatacc atccaggttg aactgaccca gagatttgtt atcagccgca
     7921 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     7981 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     8041 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     8101 ttcacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     8161 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     8221 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     8281 gcgtcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     8341 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcatctgca
     8401 gtgatgtacg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     8461 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     8521 tctttcttga attcttcaac caggtagttg atcagacggc tgtcgaagtc ttcaccaccc
     8581 aggtgggtat caccgttggt agccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     8641 atttcgataa tagaaatatc gaaagtacca ccacccaggt cataaaccgc gatagtacgg
     8701 ttgccagtac ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     8761 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     8821 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccaa gtaatcttca
     8881 gcggttttct tcattttttt cagcacttca gcagagatct gcggcggtgc cattttctgg
     8941 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     9001 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     9061 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     9121 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     9181 agcacgcgag gagtggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     9241 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     9301 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     9361 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     9421 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatata gcaggacaag acgtgaaact
     9481 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa gacattcatc aaaaaattgt
     9541 aataaaaaga aaacattacg cacctgaatc ttctctttat cagcagtaaa ctagtgggta
     9601 tccatccccc tacctctccc cactaagaga atccttatga aatccgtttt tacgatttcc
     9661 gccagcctgg cgattagtct gatgctgtgc tgcacggcgc aggcaaacga ccataaaatc
     9721 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     9781 tgccgaattg ttaaacgcat acaactctct gccgaccatg gcgatttaca gttaagcggt
     9841 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     9901 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     9961 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
    10021 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
    10081 cctgaatgat tcagatctgg attttctcca gcattgcagt gaggaacagt tggcaaattt
    10141 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagcg tactgatgcg
    10201 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
    10261 gattgctgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
    10321 cggtaaattg tatcgggcca ttttgctcga tgtttcaaaa cgattgaagc tgaaagccga
    10381 caaagagatg tctacgtttg agattgagca acagttactg gaacaatttc tgcgtaacac
    10441 ctggaagaat atggacgagg aacataaaca ggagtttctg cacgcggtcg atgcgagggt
    10501 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggcgt
    10561 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
    10621 acttgggcat ggtttgctgc gcggcgcggg gctgggaggc cctgtaggtg cggcactaaa
    10681 tggggttaaa gcggtcagcg gcagcgccta tcgcgtgacg attccagccg tactgcaaat
    10741 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccgtaaac cggggaaaat
    10801 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
    10861 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
    10921 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
    10981 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
    11041 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
    11101 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
    11161 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
    11221 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcacgc
    11281 tgaaaggcgc acgcgttctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
    11341 tggcgatcgg taacattgcc ggtaacgccg caatcatcca ctttgccggc tggattgggc
    11401 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtactgaac gagcagtttg
    11461 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtt ttgagatccc cccgctcggg
    11521 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg ctgcaaccac
    11581 ttccggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
    11641 aaaaataccg tgtaccacaa cgttacccgc agcgtccttc acaccttcca gcgtctcttt
    11701 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacaccaac
    11761 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctt atctggcgca tctgttcacc
    11821 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
    11881 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccactagctc
    11941 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
    12001 cggcgtggtt agcgccgatg tcagccattc ttccagcgca gggatgcctt tatcctgata
    12061 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
    12121 gatttttccg tttaaacagt ctcgttaaac agaatgatac actgcgaagg gagtgacaga
    12181 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
    12241 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
    12301 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
    12361 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
    12421 gctctcagcc agctctttca gcagtgtagg tgcgatggta agacggtcgc agcctgccag
    12481 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
    12541 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
    12601 gtactctttc ttatcagtat tcgctttgta ccagtcaaga atacggccaa caaatggcga
    12661 gatcaggaac acgcctgctt ccgcacaagc acgagcctga gcgaaggaga acagcagggt
    12721 caggttacag ttgatgcctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    12781 agaagccagt ttgatcagaa tacgatcgtt gctaatacca gcatcgttgt agagtttgat
    12841 caggcgtttt gctttcgcaa ttgacgcttc ggtgtcatag gaaagacgcg catcaacttc
    12901 agttgagata cgacccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    12961 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    13021 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    13081 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    13141 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    13201 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    13261 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    13321 gttgatatcg cccgccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    13381 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    13441 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    13501 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    13561 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    13621 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    13681 gtagcggcaa cctggccggc gttgcgctgg caattaccgc cggtggacct ggagccgtct
    13741 tttggatgtg ggttgccgcg tttatcggca tggcgacctc gtttgccgaa tgttctctgg
    13801 cacaacttta taaagaacgt gacgtcaatg gacagtttcg tggcggaccg gcatggtata
    13861 tggcgcgcgg gctggggatg cgttggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    13921 cctatggcat aattttcagc ggagttcagg cgaacgccgt tgcccgcgcc ctaagttttt
    13981 cttttgattt tcctccgctg gtgacaggca ttattctcgc tgtctttgct ctgctggcaa
    14041 tcactcgcgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga
    14101 taatctgggt gctgaccagc atggtgattt gcgtaataaa tatcgggcaa cttccccacg
    14161 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    14221 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    14281 cgggaatggg ttcgacgcca aacgcggcag cggcagcggc gtcctggcct ccgcatccgg
    14341 cagcgcaagg gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg
    14401 caagcgccat gctgatatta ctggcgggta acgacacaac ctacatgccg ctggaaggta
    14461 ttcagcttat ccagaaggcg atgcgggtgc taatgggttc ctggggtgct gagtttgtta
    14521 ccctcgtggt tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa
    14581 acaatctctt ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca
    14641 ccttcgcaac ggtcatcggc ggcaccttgc taagccttcc gctgatgtgg caactggcag
    14701 atatcataat ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg
    14761 tggttcatac cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt
    14821 ttgatccgtt gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg
    14881 tttcgcagga gtaattacaa ctatcgatca actcattctc tttttttgct aaagtcggca
    14941 taaatttcct gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt
    15001 accaaagccc gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc
    15061 agttgatcca tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca
    15121 gcgacaaact ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc
    15181 cggagaatgc ccgccaggcg attctagcgt ttaaaggtga tgtctacacc ggcttgcagg
    15241 ccgaaacctt cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg
    15301 gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga
    15361 tccgtcttga gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca
    15421 acaagctgaa cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag
    15481 atgagtattt taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt
    15541 tcctcgatga gaagaacggc aaatttaaga taatcagctt ctacgctaag aaagcacgcg
    15601 gtctgatgag tcgttttatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt
    15661 ttaatagcga aggttacttc tttgatgaag cttcctccag caatggcgaa ctggtgttta
    15721 aacgctacga gcagcgttaa gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc
    15781 ggcctacaaa agcatgcaga ttcaatatat tgcaga
//
LOCUS       DAZGAN020000111        15778 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGAN020000111 DAZGAN020000000
VERSION     DAZGAN020000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.111"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..1374)
                     /gene="uidB"
                     /locus_tag="ACHKYO_RS19420"
                     /old_locus_tag="pgaptmp_003884"
     CDS             complement(1..1374)
                     /gene="uidB"
                     /locus_tag="ACHKYO_RS19420"
                     /old_locus_tag="pgaptmp_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HGU4430651.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(1371..3182)
                     /gene="uidA"
                     /locus_tag="ACHKYO_RS19425"
                     /old_locus_tag="pgaptmp_003885"
     CDS             complement(1371..3182)
                     /gene="uidA"
                     /locus_tag="ACHKYO_RS19425"
                     /old_locus_tag="pgaptmp_003885"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HGU4430652.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRIFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3571..4161)
                     /gene="uidR"
                     /locus_tag="ACHKYO_RS19430"
                     /old_locus_tag="pgaptmp_003886"
     CDS             complement(3571..4161)
                     /gene="uidR"
                     /locus_tag="ACHKYO_RS19430"
                     /old_locus_tag="pgaptmp_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HGU4430653.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4390..5157)
                     /gene="hdhA"
                     /locus_tag="ACHKYO_RS19435"
                     /old_locus_tag="pgaptmp_003887"
     CDS             complement(4390..5157)
                     /gene="hdhA"
                     /locus_tag="ACHKYO_RS19435"
                     /old_locus_tag="pgaptmp_003887"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HGU4430654.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5269..6297)
                     /gene="malI"
                     /locus_tag="ACHKYO_RS19440"
                     /old_locus_tag="pgaptmp_003888"
     CDS             complement(5269..6297)
                     /gene="malI"
                     /locus_tag="ACHKYO_RS19440"
                     /old_locus_tag="pgaptmp_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HGU4430655.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6472..8064
                     /gene="malX"
                     /locus_tag="ACHKYO_RS19445"
                     /old_locus_tag="pgaptmp_003889"
     CDS             6472..8064
                     /gene="malX"
                     /locus_tag="ACHKYO_RS19445"
                     /old_locus_tag="pgaptmp_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HGU4430656.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            8074..9246
                     /gene="malY"
                     /locus_tag="ACHKYO_RS19450"
                     /old_locus_tag="pgaptmp_003890"
     CDS             8074..9246
                     /gene="malY"
                     /locus_tag="ACHKYO_RS19450"
                     /old_locus_tag="pgaptmp_003890"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HGU4430657.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9350..10351
                     /gene="add"
                     /locus_tag="ACHKYO_RS19455"
                     /old_locus_tag="pgaptmp_003891"
     CDS             9350..10351
                     /gene="add"
                     /locus_tag="ACHKYO_RS19455"
                     /old_locus_tag="pgaptmp_003891"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HGU4430658.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPSVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(10386..11426)
                     /gene="ydgJ"
                     /locus_tag="ACHKYO_RS19460"
                     /old_locus_tag="pgaptmp_003892"
     CDS             complement(10386..11426)
                     /gene="ydgJ"
                     /locus_tag="ACHKYO_RS19460"
                     /old_locus_tag="pgaptmp_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU4430659.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFAVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11669..11794
                     /gene="blr"
                     /locus_tag="ACHKYO_RS19465"
                     /old_locus_tag="pgaptmp_003893"
     CDS             11669..11794
                     /gene="blr"
                     /locus_tag="ACHKYO_RS19465"
                     /old_locus_tag="pgaptmp_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HGU4430660.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            12067..12282
                     /gene="ydgT"
                     /locus_tag="ACHKYO_RS19470"
                     /old_locus_tag="pgaptmp_003894"
     CDS             12067..12282
                     /gene="ydgT"
                     /locus_tag="ACHKYO_RS19470"
                     /old_locus_tag="pgaptmp_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HGU4430661.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            12368..12808
                     /gene="ydgK"
                     /locus_tag="ACHKYO_RS19475"
                     /old_locus_tag="pgaptmp_003895"
     CDS             12368..12808
                     /gene="ydgK"
                     /locus_tag="ACHKYO_RS19475"
                     /old_locus_tag="pgaptmp_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HGU4430662.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            12885..13466
                     /gene="rsxA"
                     /locus_tag="ACHKYO_RS19480"
                     /old_locus_tag="pgaptmp_003896"
     CDS             12885..13466
                     /gene="rsxA"
                     /locus_tag="ACHKYO_RS19480"
                     /old_locus_tag="pgaptmp_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HGU4430663.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            13466..14044
                     /gene="rsxB"
                     /locus_tag="ACHKYO_RS19485"
                     /old_locus_tag="pgaptmp_003897"
     CDS             13466..14044
                     /gene="rsxB"
                     /locus_tag="ACHKYO_RS19485"
                     /old_locus_tag="pgaptmp_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HGU4430664.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            14037..>15778
                     /gene="rsxC"
                     /locus_tag="ACHKYO_RS19490"
                     /old_locus_tag="pgaptmp_003898"
     CDS             14037..>15778
                     /gene="rsxC"
                     /locus_tag="ACHKYO_RS19490"
                     /old_locus_tag="pgaptmp_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HGU4430665.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEP"
BASE COUNT         3752 a         4097 c         3935 g         3994 t
ORIGIN      
        1 ttaattagtg atatcgctga ttaattgctg ctgcactttt ttacgattat caatttcaac
       61 cacgatttct ttgaattttt tatccgtgag cggataaaac cagataataa cgaatgccag
      121 tagcataaat ccgcaaggta ctaaggcaat tgatgtgcgg atgcccataa taacttccgg
      181 cgtttgcacc tgattggcga tatatccgct taatcccaaa ataaaggcag gaattgaacc
      241 tccgattgcc tgaccgcatt tacgggtaaa tgagaatagt gaataggtga gcccttcaat
      301 tcgcacgccg gtcaggtatt caccgtattc tacggtatca gcttccagtg cccacatcac
      361 agtcatagta acgccctgac caattgaagc gatggccaac gcaaccaacg ccaccggcag
      421 tgaccagacg gaaacccaga agaatagcag ataaccgcag gttcccagca aagcgccaat
      481 caggaaggta ttctttttac cgatcctcgc gaccatcccc ggcaccagcg gtgccgatgc
      541 cacagtacca accaggtttt gcaccagtac cagcacagtg aacagcccgg tatcatttaa
      601 cacatagcgc acgtagaaca acgacgaggc gctgaccgcg aaggtcgaaa tcagcacaca
      661 cagcgcaccg atgcacaaca taaacagcgg gcgattccgt ttcagggttt gcagactgat
      721 cttcaatgac ggctgcgcca cgatacgtac cacattctca cgcgtcgatt tgaagcagat
      781 gaagtaaagc accattccgg caatcgccag cacaatcgtc cagaaatggt ataccgacac
      841 catctcttcc gggctggagt tcttaatgct cggtcctatc agaaatgcca ggcagacaaa
      901 ggttaatgaa gcggcaatcc cgcgagccgc gcccagacgg gcgcgggatt gtggttgttg
      961 ggtcatcgcg gtagcaagtg aaccataagg aatattcacc aggctgtagc aaagcccgag
     1021 gcccatgtag gtcaaatatg catacactac tttgctgcta tggctccagt cggtcggtac
     1081 ccagaatacc agcacgctga agatcattaa cggcgcagta ccgaagagta aaaacgggcg
     1141 gaattttccc cagcgggtat tcacactgtc caccactcgt ccggcaaaga cgtcggcgaa
     1201 ggcatcgaat acccgcacca gtaacagcat ggtgcccgcc gcagcggcac cgacgccagc
     1261 gacgtcggtg tagtaactca acaggaagag cgcccccatt gcgaaggcga agttattggc
     1321 gacgtcaccg aggctgtagc cgacgatggt gcgccaggag agttgttgat tcattgtttg
     1381 cctccctgct gcggtttttc accgaagttc atgccagtcc agcgtttttg cagcagaaaa
     1441 gccgccgact tcggtttgcg gtcgcgagtg aagatccctt tcttgttacc tccaacgcgc
     1501 aatatgcctt gcgaggtcgc aaaatcggcg aaattccata cctgttcacc gacgacggcg
     1561 ctgacgcgat caaagatgcg gtgatacata tccagccatg cacactgata ctcttcactc
     1621 cacatgtcgg tgtacattga gtgcagcccg gctaacgtat ccacgccgta ttcggtgatg
     1681 ataatcggct gatgcagttt ctcctgccag gccagaagtt ctttttccag taccttctct
     1741 gccgtttcca aatcgccgct ttggacatac catccgtaat aacggttcag gcacagcaca
     1801 tcaaagagat cgctgatggt atcggtgtga gcgtcgcaga acattacatt gacgcaggtg
     1861 atcggacgcg tcgggtcgag tttacgcgtt gcttccgcca gtggcgcgaa atattcccgt
     1921 gcaccttgcg gacgggtatc cggttcgttg gcaatactcc acatcaccac gcttgggtgg
     1981 tttttgtcac gcgctatcag ctctttaatc gcctgtaagt gcgcttgctg agtttccccg
     2041 ttgactgcct cttcgctgta cagttctttc ggcttgttgc ccgcttcgaa accaatgcct
     2101 aaagagaggt taaagccgac agcagcagtt tcatcaatca ccacgatgcc atgttcatct
     2161 gcccagtcga gcatctcttc agcgtaaggg taatgcgagg tacggtagga gttggcccca
     2221 atccagtcca ttaatgcgtg gtcgtgcacc atcagcacgt tatcgaatcc tttgccacgc
     2281 aagtccgcat cttcatgacg accaaagcca gtaaagtaga acggtttgtg gttaatcagg
     2341 aactgttcgc ccttcactgc cactgaccgg atgccgacgc gaagcgggta gatatcacac
     2401 tctgtctggc ttttggctgt gacgcacagt tcatagagat aaccttcacc cggttgccag
     2461 aggtgcggat tcaccacttg caaagtcccg ctagtgcctt gtccagttgc aaccacctgt
     2521 tgatccgcat cacgcagttc aacgctgaca tcaccattgg ccaccacctg ccagtcaaca
     2581 gacgcgtggt tacagtcttg cgcgacatgc gtcaccacgg tgatatcgtc cacccaggtg
     2641 ttcggcgtgg tgtagagcat tacgctgcga tggatcccgg catagttaaa gaaatcatgg
     2701 aagtaagact gctttttctt gccgttttcg tcggtaatca ccattcccgg cgggatagtc
     2761 tgccagttca gttcgttgtt cacacaaacg gtgatacgta cacttttccc ggcaataaca
     2821 tacggcgtga catcggcttc aaatggcgta tagccgccct gatgctccat cacttcctga
     2881 ttattgaccc acactttgcc gtaatgagtg accgcatcga aacgcagcac gatacgctgg
     2941 cctgcccaac ctttcggtat aaagacttcg cgctgatacc agacgttgcc cgcataatta
     3001 cgaatatctg catcggcgaa ctgatcgtta aaactgcctg gcacagcaat tgcccggctt
     3061 tcttgtaacg cgctttccca ccaacgctga tcaattccac agttttcgcg atccagactg
     3121 aatgcccaca ggccgtcgag ttttttgatt tcacgggttg gggtttctac aggacgtaac
     3181 ataagggact cctcattaag ataaaaatac tggtcaacct ttaatctgat tagttaacca
     3241 attctgtgtt gcacacagtt atctgtgaga aacccatgga agagaaatgg aaataaaaag
     3301 aactgacacc gcgatcaaaa aaccaaaaac tgcaataccc acagcgtgat attgatcgca
     3361 ttaatcttta aaacggcgtt gacagaagcc ataaaataag caaccgaacg ttcgttcgag
     3421 tatattggga acgataaact ctcatgacat atggctacgg tgaatatttt tgggggggaa
     3481 ttctccgatc ccggctagat tgtattttgt acagggaaat taaagtatga aacgcgggca
     3541 cagttatcac cagcccgcga aatgagagga tcaggatgcg gttaagatac cgccaatcat
     3601 cgcgcgtaat ccctgagcaa acgacgcttc acgcgccaga tcttccgctt cgatatcggc
     3661 cagtacaccg taggtcagat ccagcagtaa acgtgaagtc attgacgtgt tgatgtctgg
     3721 gcttatctcg cctttttgct gggcatcacg catccgctgg gcaacaaatt cagtgatcgt
     3781 catatgctta tttttaagca tggcggcgac ctgtgggtta cgcatccctt ccgccataat
     3841 ttcgaccacc agcgcccgtt gcccaaaggc ttcatgggtg agagagacaa tagactcgac
     3901 catatagtca acaaaatgaa tcccttcaat cggttcccgg aaacgggcca gcgccctctc
     3961 ctggtcctgt aagataatcg cctgaatcaa ggcttctttg gagatgaaat ggtgatagag
     4021 cgtcccggga ctaatggcgc aggatttaca gatggctttc atcgaggcac tgtgaaatcc
     4081 attttctgaa aaaatctctc tggcagcatt gaggatccgg gtccgtgtcg gttgtgcttc
     4141 agtctgcatg ttatccatca ttaaaccatt atagttagcg tgaataacta gtttaccgcg
     4201 ttgtcaggtg agcgcctatc attgctgttg aatagtgcga aggcacactc tattcgtatg
     4261 cttttcatcc agctattggt taaccaatag ccgtgatttg gctgccagtt gtttagcaga
     4321 gacgttgatg aattgcacac gctatcccgg taaacaacac atacggctgt ttaccggttc
     4381 gttagtgtat taattgagct cctgtacccc accaccggag acggtgagaa tttgtccgct
     4441 tacccagctg gctgcaggcg agcaaaggaa cagcgccgcg ttagcaatat cttgcggttg
     4501 gcccagacgt ctgatcggcg tgtgctgtaa cattttttgt tcaatttctg gtgtaataac
     4561 ggatttcagg gcatcggtta atattgcccc cggcgcaatg ccattcaccc gaatattttt
     4621 ttcacccagg tcaaatgcca tatttctgac cagatgactg gccgcagctt tagatgatgc
     4681 ataggaagtc atgtttatat ttttattttc tgccgccata gaagtgatgg tcagaataac
     4741 gccaccgcca tttttttcca tttctggcgc aacaagttgt gacagatgga aaaaagaaaa
     4801 cacattcagt tcataagcac ggcgaaaatc tgccattggc atatcaaacg gtttaggacc
     4861 accgccaccg gcgttgttaa ccaggatatc aactttaccc agcttactga cggcaaagtc
     4921 tgccagtgca gagagttcct gttcggaagt aatatcacaa cggcaggcaa atgcctgacc
     4981 acccagttgt tgaatttcgt ctacaacatg gttagctgcg tcggcgttaa tatcactgac
     5041 caccacagat gcgccagctg tcgcgaatgt aatggcgatt tctttaccaa tacctgcacc
     5101 cgcacctgtg atgatggcgc attttccgtc gagtctcagg ttgtcagaat taaacacata
     5161 aacctcctgt gaacttcagt aaaaataaaa ggcaccgcct gtgtgccaca ttttttatca
     5221 ttaaccttga ggaaaggaca ctaccaggta tagcagataa agagaggatt atttcgccgc
     5281 aatgagccgg gcgggaataa taagattgcg tgaatgcgtc tcttcatggg tgattttttg
     5341 catcatgcga tccgcaagtg tggtaccaag ttcccgcgca ggcgtgctgg cccaggtaac
     5401 gggtatatca tcaagtgtgg ttggtgtcgc atcggtaaat gccgccagcg aaacttgttg
     5461 ctcaaagtaa cgatcgactc cgctttcccc gctttgccgc cctgctttga gcaaaccaaa
     5521 ccatgccccc atcgcaatag tttcgttata gcaaaccaca gcactgatgg tcgggttatg
     5581 acgtaaaagc gccgtgatag cttccgcggc ttgcttctgg ctggatgtgc actccagcac
     5641 ccaatcgctg tgaaacggca ggccaaattt tagtagagtt gcacaatagc cccccacccg
     5701 ttctgcacgg gttaatgagg aactttgccc tcccagccag gcgatccgct gatgcccatt
     5761 gcgaatgaga tgctccgtca acaactgtgc agcctgcatg ttgtccgggc gaaccgtatc
     5821 aacatcatca agataactgg cacgggaagc gaatatcacc gggatagctt tttcttctgc
     5881 catccgtcgc aggtcatcgc tgcttcctgc agccccggca attaccacac catccacacc
     5941 ctgattcagt aacagtgaaa accgctgtgc cagctgctcg ccgtctttac cgccgtgaag
     6001 caaaaaaacc atccgtccct gcgcttccag agcttccgtc aatccggccg tcaattcggc
     6061 gtaaaacggc gcagataaat cacggacgat caaaccaatg acgccgcttt gcccgccgcg
     6121 cagcgccgac gcctggcgat tgcgcacaaa tcccagctct tcaatggcgg cgttaacgcg
     6181 ttctcctgtg gcggtagaga ttcgcccttt gccactaagc accagcgaaa cggtacttac
     6241 cgacacgccc gcagccagcg caacatcatg aatggttatt tttttggcgg tagccatgca
     6301 gatgacctac tccctgatta tgtgacagat aaaacgtttt accttttatt tatcttatac
     6361 ccgctattat cgttgcgtaa tgtgatttat gcctcactaa aatttgataa aacgttttat
     6421 cttctcgcgc aatttactga atccagattg ttctctacga ggagtcgttt tatgacggcg
     6481 aaaacagcac cgaaagtcac gctgtgggag ttcttccagc agttaggcaa aaccttcatg
     6541 ttacccgtgg cattattgtc gttctgcggc attatgctcg gcattggtag ttctcttagc
     6601 agccatgatg tcataaccct gatcccggtc ctgggcaacc ccgtgttgca ggctatcttt
     6661 acctggatga gtaagattgg ctcgtttgct tttagtttcc tgcctgtcat gttctgtatc
     6721 gccatcccgc tgggcctggc acgcgaaaat aaaggcgtag cggcattcgc tggcttcgtc
     6781 ggttatgcgg taatgaacct cgcggtaaac ttctggttga ccaataaagg cattctgcca
     6841 accacggatg ccgcggttct gaaagccaat aacatccaga gcattcttgg gatccagtcg
     6901 atcgataccg ggatcctcgg tgcggtgatc gccggtatta tcgtctggat gctgcatgag
     6961 cgtttccata atatccgcct gccggatgcg ctggcattct tcggcggtac gcgcttcgta
     7021 ccaattatct cctcgctggt gatgggcctt gtcggcctgg tgattccatt agtctggccg
     7081 attttcgcca tgggtattag cggcttgggc catatgataa acagcgcggg tgatttcgga
     7141 ccgatgctgt ttggtaccgg tgaacgtctg ctgttgccgt ttggtctgca tcacattctg
     7201 gtagcattaa ttcgctttac cgacgcaggc ggcacgcagg aagtctgcgg tcaaaccgtc
     7261 agcggcgcac tgaccatctt ccaggcgcaa ttgagttgcc cgaccactca cggtttttct
     7321 gaaagcgcca cgcgtttcct ttcgcaaggt aaaatgcctg cgtttctcgg cggtctgccg
     7381 ggtgcagcgt tagctatgta tcactgcgcg cgcccggaaa atcgccataa aattaaaggt
     7441 ctgctgattt ctggcctgat cgcctgcgtc gttggcggca ctaccgaacc gctggaattc
     7501 ctgttcctgt tcgtagcgcc agttctgtat gtcatccacg cgctgttaac cggcctcggc
     7561 ttcaccgtca tgtctgtgct cggcgtcacc atcggtaata ccgacggcaa tatcatcgac
     7621 ttcgtggtgt tcggtatttt gcatggtctg tcaaccaagt ggtacatggt gccagtggtg
     7681 gcggcaatct ggtttgtcgt ttactacgtc atcttccgtt tcgctatcac ccgcttcaat
     7741 ctgaaaaccc cggggcgcga tagcgaagtt gccagctcaa tcgaaaaagc cgttgccggt
     7801 gcgccgggta aatcaggtta caacgttcct gcaatcctcg aagcattagg cggtgccgac
     7861 aatattgtca gcctcgataa ctgcattacc cgtctgcgtt tgtctgtgaa agatatgtcg
     7921 cttgttaatg tgcaggcact gaaggacaat cgggcaattg gcgtagtaca acttaatcaa
     7981 cataacctgc aggttgttat cgggccacaa gttcagtcag taaaagatga aatggccggt
     8041 ctgatgcata ctgtccaggc ataaggataa gatatgttcg atttttcaaa ggtcgtggat
     8101 cgtcatggca catggtgtac acagtgggat tatgtcgctg accgtttcgg cactgctgac
     8161 ctgttaccgt tcacgatttc agacatggat tttgccactg ccccctgcat tatcgaggcg
     8221 ctgaatcagc gcctgatgca cggcgtattt ggctacagcc gctggaaaaa cgatgagttt
     8281 ctcgcggcta ttgcccactg gttttccacc cagcattaca ccgcaattga tacgcagacg
     8341 gtggtgtatg gcccttctgt catctatatg gtttcagaac tgattcgtca gtggtctgaa
     8401 acaggtgaag gcgtggtgat ccacacgccc gcctatgacg cattttacaa ggccattgaa
     8461 ggtaaccagc gcacagtaat gcccgttgct ttagagaagc aggctgacga ctggttttgc
     8521 gatatgggca agctggaagc cgtgttggcg aaaccagaat gtaaaattat gctcctgtgt
     8581 agcccacaga atcctaccgg gaaagtgtgg acgtgcgatg agctggagat catggctgac
     8641 ctgtgcgagc gtcatggtgt gcgggttatt tccgatgaaa tccatatgga tatggtctgg
     8701 ggcgagcagc cgcatattcc ctggagtaat gtggcgcggg gagactgggc gttgctgacg
     8761 tcgggctcga aaagtttcaa tattcccgcg ctgaccggtg cttacgggat tatagaaaat
     8821 agcagcagcc gtgatgccta tttatcggca ctgaaaggac gtgatgggct ttcttcccct
     8881 tcggtactgg cgttaactgc ccatatcgcc gcctatcagc aaggtgcacc gtggctggat
     8941 gccttacgcg tctatctgaa agataacctg acgtatatct cagataaaat gaacgccgcg
     9001 tttcctgaac tcaactggca gatcccgcaa tccacttatc tggcctggct tgatctacgt
     9061 ccgttgaata ttgacgacaa cgcgttgcaa aaagcgctta tcgagcaaga aaaagtcgcg
     9121 atcatgccgg ggtataccta tggtgaagaa ggtcgtggtt ttgtccgtct caatgccggc
     9181 tgcccacgtt cgaaactgga aaaaggtgtg gctggattaa taaacgccat ccgcgctgtt
     9241 cgctaacccc aattgcgcaa cgtaaaaaat cgttgcgcaa tcgtggattt ttaccctgct
     9301 ttgtttttat aatggtgcgc acttttatat tcagaaaaag agtgcgacca tgattgatac
     9361 caccctgcca ttaactgata tccatcgcca ccttgatggc aacattcgtc cccagaccat
     9421 tcttgaactt ggccgccagt ataatatctc gcttcctgca caatccctgg aaacactgat
     9481 tccccacgtt caggtcattg ccaacgaacc cgatctggtg agctttctga ccaaacttga
     9541 ctggggcgtt aaagttctcg cctctcttga tgcctgtcgc cgcgtggcat ttgaaaacat
     9601 tgaagatgca gcccgtcacg gcctgcacta tgtcgagctg cgtttttcac caggctacat
     9661 ggcaatggca catcagctgc ctgtagcggg tgttgtcgaa gcggtgatcg atggcgtacg
     9721 tgaaggttgc cgcacctttg gtgtgcaggc gaagcttatc ggcattatga gccggacctt
     9781 cggcgaagcc gcctgtcagc aagagctgga ggccttttta gcccaccgtg accagattac
     9841 cgcacttgat ttagccggtg atgaacttgg tttcccggga agtctgttcc tttctcactt
     9901 caaccgcgcg cgtgatgcgg gctggcatat taccgtccat gcaggcgaag ctgccgggcc
     9961 ggaaagcatc tggcaggcga ttcgtgaact gggtgcggag cgtattggac atggcgtaaa
    10021 agccattgaa gatcgggcgc tgatggattt tctcgccgag caacaaattg gtattgaatc
    10081 ctgtctgacc tccaatattc agaccagcac cgtagcagag ctggctgcac atccgctgaa
    10141 aacgttcctt gagcatggca ttcgtgccag cattaacact gacgatccca gcgtacaggg
    10201 agtggatatc attcacgaat ataccgttgc cgcgccagct gctgggttat cccgcgagca
    10261 aatccgccag gcacagatta atggtctgga aatggctttc ctcagcgctg aggaaaaacg
    10321 cgcactgcga gaaaaagtcg ccgcgaagta acaaaatgga tggtgcaaat tgcaccatcc
    10381 atttttcatg caaggcacaa agtcgcgcga tgtttggcgg attcgatgcc cagctcaatc
    10441 aactccatta cctggattgc ctggcttgcc ggaaccggat tttcaccatc gccatttaac
    10501 gcatcacgaa tagccgcata gtaagccgga taattcccag gcaccgtcaa cagcgtttct
    10561 tcgacacgtt cctcaccttc cacgcgggta agtacgccat cacgcatatc gtagccccag
    10621 tcttcctgcg gtagacgctc gccatttttc agacgttctt cctgtggatc gaggccatat
    10681 ttcacataac tgcctcggga tccatgcacg atataccgtg ctgactcagc ggctgccagc
    10741 atggtaccgt gtaaaatgac tcgccgctgt ggataggaca agatggcgtg gaaataatcg
    10801 gtcgactgcg ctccgggccg taactgtgcc aaatctaccg tcatgctgac cggtaaacca
    10861 aaaagcgtaa tggcctgatc aagaagatgt ggtgctaaat cgtaccagat accgctgcct
    10921 gggccgccct gttcacgcca acgatcgcgc acctgcggac ggaagcggtc aaaatgagac
    10981 tcaaagtaag caacttcacc cagcacgcct tccgcgagta aaccttttag cgtcaagaaa
    11041 tcgctatccc agcgacggtt atggaataca gacagcacac gccccaggct ttttgccagc
    11101 gcatccagct ctcgcgcttg tgacagtgtc acggcaaagg gtttatcaac gaccacatgt
    11161 ttacccgcct caagcgccgc tttggctaac gggaaatggg tatcgttggg tgtaggaatg
    11221 acaatcaggt ctatgttggg atcgttaaac agatgcttcg gctcagagac aaccgtaacc
    11281 gttggccagt cggcttttac ttttgtttca tcactgctgg agattaccgc cagttccagc
    11341 ccgggcgtgc ccgcaatcag gggcgcatgg aaggttttgc tcgcataacc atacccaatc
    11401 aacccaacac ggatgttgtc gctcatgtca ttgcctctca ttgaagtatg atggctattt
    11461 gacactatcc tttacccacg ctcaacagtt taataacctg ccagcaataa gggatgttgt
    11521 ttaacttaag tcaaaaaaat agcgaatttt ccaacgacaa aagctaaata tcgcaaaaac
    11581 ctcagtaaaa atcttgctgg agctattatt gctaagtaac atttaccccc tgaagttaat
    11641 ggatcaatca agagagatgt gggctgtaat gaatcgtctt attgaattaa caggttggat
    11701 cgttcttgtc gtttcagtca ttcttcttgg cgtggcgagt cacattgaca actatcagcc
    11761 acctgaacag agtgcttcgg tacaacacaa gtaagctctg cacttgtgga gcgacatgct
    11821 gcccgtccgg gtgcatgttt tcacttgtcg gatattaaac caggaattta ttatcttgtt
    11881 cgatgttgtt ggtgattgtc agggatagta aagcgcgtta ctcttttcag agcagttttt
    11941 catccctgaa tatttgccat cagcgcattc ttattattgt aataacacaa gcgttcactt
    12001 ttggtgaaat tttatcatca ctaataatac ctaccccttt ggtattaatt attttatgga
    12061 ccttttatga ctgttcagga ctacttatta aaatttcgca aaatcagttc actcgaaagt
    12121 ctggaaaaac tctacgacca tcttaattac accctgacgg acgatcagga actgatcaat
    12181 atgtatcgtg ctgccgatca ccgtcgcgca gagctggttt ctggcgggcg tttgtttgac
    12241 ctcggccagg taccgaagtc cgtctggcac tatgtccaat aaagaaagta gcgatcggtt
    12301 ataaaagcct tataataacg cccctaaaat tagatttgcc tgtttgcgtc gctattctgg
    12361 agaacctatg accaccacaa cgccacaacg tattggaggc tggttgcttg gcccgttggc
    12421 ctggctttta gtcgctctgt tgagtacgac gctggcgttg ttgctgtata ccgctgcgtt
    12481 atcttctccg caaacatttc aaacgctcgg cggacaagca ctgacgacac aaatcttatg
    12541 gggcgtttct tttattaccg ctatcgccat gtggtattac acactgtggt tgaccatcgc
    12601 tttctttaaa cgtcggcgtt gtgttcctaa acactatatt atctggctgc ttatttccgt
    12661 attgctggca gtaaaagcat tcgctttttc acccgtggaa gatggcatag ccgttcgcca
    12721 gttgctgttt actttgttgg caaccgcatt gatcgtcccc tatttcaaac gctcgtcgcg
    12781 ggtaaaagcg acgtttgtga atccgtaata accttacagt taacctgttg tcgcctgctc
    12841 tggattaacg gataataggc ggctttttta tttcaggccg aaaaatgact gactacctgt
    12901 tactgtttgt cggaactgta ctggtcaata actttgtact ggtcaagttt ctcggtctct
    12961 gtccgtttat gggggtttcc aaaaaactgg aaaccgcgat gggcatgggg ctggcaacaa
    13021 cgtttgtgat gacgctggcg tctatttgcg cctggcttat cgatacgtgg attttgatcc
    13081 cacttaatct gatttacctg cgcaccctgg catttattct ggtgattgct gtggtcgtgc
    13141 agttcaccga gatggtggtg cgcaaaacca gcccggtgct ttaccgtctg ctggggattt
    13201 ttttgccgct tatcaccacc aactgtgcag tgctcggcgt ggcgttgctg aatatcaatc
    13261 tcgggcacaa tttcttgcag tcggcgctgt acggtttttc cgccgctgtc ggtttctcgc
    13321 tggtgatggt gctcttcgcc gccatccgcg aacgccttgc tgtggctgat gtcccggcac
    13381 cttttcgcgg taatgccatt gcgttaatta ccgcaggtct tatgtctctg gcctttatgg
    13441 gctttagtgg tttggtgaag ttgtaatgaa tgctatctgg attgccgttg ccgccgtgag
    13501 cctgctgggc ctggcgtttg gcgccattct gggttatgcc tcccgccgtt ttgcggtgga
    13561 agacgatccg gtcgttgaga aaattgacga aatcttaccg cagagccagt gtggtcagtg
    13621 cggttatccc ggctgtcgcc cctacgcgga agccatcagc tgtaacggtg aaaaaatcaa
    13681 ccgttgcgcc ccaggtggcg aagctgtgat gctaaaaatt gccgagttgc ttaatgtcga
    13741 gccgcagccg ctggatggcg aagcgcaaga gctaacgcct gcgcggatgg tggcggttat
    13801 tgatgaaaat aactgtattg gctgcactaa atgtattcag gcttgtccgg tagacgccat
    13861 cgttggcgct acccgtgcca tgcatacggt aatgagtgat ctctgtacgg gctgcaattt
    13921 atgtgttgat ccgtgcccga cgcactgcat ctcgttgcaa ccggtcgcag aaacacctga
    13981 ctcctggaaa tgggatctga acaccattcc cgtgcgtatc attcccgtgg aacaccatgc
    14041 ttaagttatt ctctgcattc agaaaaaata aaatctggga tttcaacggc ggcatccatc
    14101 caccggagat gaaaacccag tccaacggta cacccctgcg ccaggtacca ctggcgcagc
    14161 gttttgttat tccactgaaa cagcatattg gcgctgaagg tgagttgtgc gttagcgtcg
    14221 gcgataaagt attgcgcggc cagccgctta cccgtggtcg cggcaaaatg ctgcctgttc
    14281 acgcgcccac ctcgggtacc gttacggcta ttgcgcccca ctctacggct catccttcag
    14341 ctttagctga attaagcgtg attattgatg ccgatggtga agactgctgg atcccgcgcg
    14401 acggctgggc cgattatcgc actcgcagtc gcgaagagtt aatcgagcgc atacatcagt
    14461 ttggtgtagc cgggctgggc ggtgcaggat tcccgacagg cgttaaattg cagggtggcg
    14521 gagataagat tgaaacgttg attatcaacg cggctgagtg cgagccgtac attaccgccg
    14581 atgaccgttt gatgcaggat tgcgcggctc aggtcgtaga gggtattcgc attcttgcgc
    14641 atattctgca gccacgcgaa attcttatcg gcattgaaga taacaaaccg caggcgattt
    14701 ccatgctgcg cgcggtgctg gcggactcta acgatatttc tctgcgggtg attccaacca
    14761 aatatccttc tggcggtgct aaacaattaa cctacattct gaccgggaag caggttccac
    14821 atggcgggcg ttcatccgat atcggcgtat taatgcaaaa cgtcggcact gcttatgcag
    14881 tgaaacgtgc cgttattgat ggcgagccga ttaccgagcg tgttgtaacc ctgactggcg
    14941 aagcaatcgc tcgcccgggc aacgtctggg cacggctggg gacgccagtg cgtcatttat
    15001 tgaatgatgc cggattctgc ccctctgccg atcaaatggt gattatgggt ggcccgctaa
    15061 tgggctttac cttgccatgg ctggatgtcc cggtcgtaaa gattaccaac tgtctgttgg
    15121 ctccctctgc caatgaactt ggcgaaccac aggaagaaca aagctgcatc cggtgtagcg
    15181 cctgtgctga cgcctgccct gcggatcttt tgccgcaaca gttgtactgg ttcagcaaag
    15241 gtcagcaaca cgataaagct accacgcata acattgctga ttgcattgaa tgtggggctt
    15301 gcgcgtgggt ttgcccgagc aatattcccc tggtgcaata tttccgtcag gaaaaagctg
    15361 aaattgcggc tattcgtcag gaagaaaagc gcgccgcaga agccaaagcg cgtttcgaag
    15421 cgcgccaggc tcgtctggag cgcgaaaaag cggctcgcct tgaacgacat aagagcgcag
    15481 ccgttcaacc tgcagccaaa gataaagatg cgattgctgc cgctctggcg cgggtgaaag
    15541 agaaacaggc ccaggctaca cagcctattg tgattaaagc gggcgaacgc ccggataaca
    15601 gtgcaattat tgcagcacgg gaagcccgta aagcgcaagc cagagcgaaa caggcagaac
    15661 tgcagcaaac taacgacgca gcaaccgttg ctgatccacg taaaactgcc gttgaagcag
    15721 ctatcgcccg cgccaaagcg cgcaagctgg aacagcaaca ggctaatgcg gaaccaga
//
LOCUS       DAZGAN020000112        15688 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGAN020000112 DAZGAN020000000
VERSION     DAZGAN020000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.112"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            32..598
                     /gene="yceJ"
                     /locus_tag="ACHKYO_RS19495"
                     /old_locus_tag="pgaptmp_003899"
     CDS             32..598
                     /gene="yceJ"
                     /locus_tag="ACHKYO_RS19495"
                     /old_locus_tag="pgaptmp_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU4430666.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            602..1177
                     /gene="yceI"
                     /locus_tag="ACHKYO_RS19500"
                     /old_locus_tag="pgaptmp_003900"
     CDS             602..1177
                     /gene="yceI"
                     /locus_tag="ACHKYO_RS19500"
                     /old_locus_tag="pgaptmp_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HGU4430667.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1219..2271)
                     /gene="trhO"
                     /locus_tag="ACHKYO_RS19505"
                     /old_locus_tag="pgaptmp_003901"
     CDS             complement(1219..2271)
                     /gene="trhO"
                     /locus_tag="ACHKYO_RS19505"
                     /old_locus_tag="pgaptmp_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGU4430668.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDVLFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2496..3416
                     /gene="lpxL"
                     /locus_tag="ACHKYO_RS19510"
                     /old_locus_tag="pgaptmp_003902"
     CDS             2496..3416
                     /gene="lpxL"
                     /locus_tag="ACHKYO_RS19510"
                     /old_locus_tag="pgaptmp_003902"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HGU4430669.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3588..4814
                     /gene="mdtG"
                     /locus_tag="ACHKYO_RS19515"
                     /old_locus_tag="pgaptmp_003903"
     CDS             3588..4814
                     /gene="mdtG"
                     /locus_tag="ACHKYO_RS19515"
                     /old_locus_tag="pgaptmp_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HGU4430670.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4897..5271
                     /gene="msyB"
                     /locus_tag="ACHKYO_RS19520"
                     /old_locus_tag="pgaptmp_003904"
     CDS             4897..5271
                     /gene="msyB"
                     /locus_tag="ACHKYO_RS19520"
                     /old_locus_tag="pgaptmp_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU4430671.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5272..5499)
                     /gene="yceK"
                     /locus_tag="ACHKYO_RS19525"
                     /old_locus_tag="pgaptmp_003905"
     CDS             complement(5272..5499)
                     /gene="yceK"
                     /locus_tag="ACHKYO_RS19525"
                     /old_locus_tag="pgaptmp_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU4430672.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(5672..8215)
                     /gene="mdoH"
                     /locus_tag="ACHKYO_RS19530"
                     /old_locus_tag="pgaptmp_003906"
     CDS             complement(5672..8215)
                     /gene="mdoH"
                     /locus_tag="ACHKYO_RS19530"
                     /old_locus_tag="pgaptmp_003906"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HGU4430673.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8208..9743)
                     /gene="mdoG"
                     /locus_tag="ACHKYO_RS19535"
                     /old_locus_tag="pgaptmp_003907"
     CDS             complement(8208..9743)
                     /gene="mdoG"
                     /locus_tag="ACHKYO_RS19535"
                     /old_locus_tag="pgaptmp_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HGU4430674.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10137..11294
                     /gene="mdoC"
                     /locus_tag="ACHKYO_RS19540"
                     /old_locus_tag="pgaptmp_003908"
     CDS             10137..11294
                     /gene="mdoC"
                     /locus_tag="ACHKYO_RS19540"
                     /old_locus_tag="pgaptmp_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HGU4430675.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11302..12783)
                     /gene="clsC"
                     /locus_tag="ACHKYO_RS19545"
                     /old_locus_tag="pgaptmp_003909"
     CDS             complement(11302..12783)
                     /gene="clsC"
                     /locus_tag="ACHKYO_RS19545"
                     /old_locus_tag="pgaptmp_003909"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HGU4430676.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRMFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12725..13258)
                     /gene="ymdB"
                     /locus_tag="ACHKYO_RS19550"
                     /old_locus_tag="pgaptmp_003910"
     CDS             complement(12725..13258)
                     /gene="ymdB"
                     /locus_tag="ACHKYO_RS19550"
                     /old_locus_tag="pgaptmp_003910"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HGU4430677.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13353..13664)
                     /gene="ymdA"
                     /locus_tag="ACHKYO_RS19555"
                     /old_locus_tag="pgaptmp_003911"
     CDS             complement(13353..13664)
                     /gene="ymdA"
                     /locus_tag="ACHKYO_RS19555"
                     /old_locus_tag="pgaptmp_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HGU4430678.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13785..14117)
                     /gene="csgC"
                     /locus_tag="ACHKYO_RS19560"
                     /old_locus_tag="pgaptmp_003912"
     CDS             complement(13785..14117)
                     /gene="csgC"
                     /locus_tag="ACHKYO_RS19560"
                     /old_locus_tag="pgaptmp_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HGU4430679.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14176..14631)
                     /gene="csgA"
                     /locus_tag="ACHKYO_RS19565"
                     /old_locus_tag="pgaptmp_003913"
     CDS             complement(14176..14631)
                     /gene="csgA"
                     /locus_tag="ACHKYO_RS19565"
                     /old_locus_tag="pgaptmp_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HGU4430680.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14672..15127)
                     /gene="csgB"
                     /locus_tag="ACHKYO_RS19570"
                     /old_locus_tag="pgaptmp_003914"
     CDS             complement(14672..15127)
                     /gene="csgB"
                     /locus_tag="ACHKYO_RS19570"
                     /old_locus_tag="pgaptmp_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HGU4430681.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3929 a         4006 c         3702 g         4051 t
ORIGIN      
        1 tttctctcgc aaattcgttt tggatgcact tatgtcattc acaaataccc ctgaacgcta
       61 tggtgttata tctgcggcct tccattggct aagcgcaatt attgtctacg gcatgtttgc
      121 tttaggtctg tggatggtca cgctcagtta ttacgatggc tggtatcaca aagcacccga
      181 actgcataaa agcatcggta ttttgttaat gatggggctg gttattcgcg ttctgtggcg
      241 tgtcatatct ccaccgccgg gtccgctgcc gagttattcg cccatgactc gtcttgccgc
      301 aaaagctgga catctcgcat tgtacctatt gctgttcgcc atcggcatca gcggctatct
      361 gatctcaact gccgatggta aaccgatcag cgtttttggc tggtttgacg tccccgcgac
      421 ccttgccgac gctggcgcac aggcagactt tgccggtgcc ctgcattttt ggctcgcgtg
      481 gagcgtcgta gtactgtccg ttatgcacgg atttatggcc ctgaagcatc atttcatcga
      541 taaagacgac actctgaagc gcatgctggg aaagtcgtca tctgactatg gagtataaaa
      601 aatgaaaaaa agcctgcttg gtttaacctt cgcgtccctg atgttctctg ccggttcagc
      661 ggttgccgcc gattacaaaa ttgacaaaga aggtcagcac gcctttgtta atttccgcat
      721 ccagcacctt ggctatagct ggttatacgg cacctttaaa gatttcgacg gtacttttac
      781 ctttgacgaa aaaaatccgg ctgccgataa agtgaatgtg acaattaaca ccaccagcgt
      841 cgatactaat cacgccgaac gcgataaaca tcttcgcagt gcagatttcc tcaataccgc
      901 aaaatatcca caggcaacat tcacctccac cagcgtgaag aaagacggtg acgaactgga
      961 tattaccggc gatctgacgc tgaatggcgt aaccaaaccc gtcacgctgg aagcgaaatt
     1021 aattggtcag ggcgacgacc catggggtgg taaacgtgca ggcttcgagg ccgaaggcaa
     1081 aattaagctc aaggacttca atatcaagac agatttaggt ccagcttctc aggaagtgga
     1141 tctgattatt tcagtggaag gcgtacagca gaagtaagcg tgaaatgttg ccggatggca
     1201 cgcatccggc aatgatattt attctgttgg atcaggaatg cccagtgttg tattcagacg
     1261 tccacgagac ttattaaaga tcttattgcc attttcacgt cctgcccgac ggcgtcgctg
     1321 ttcttctggc ggtaacgcgc tttcttcgca gcaaatctca ctacaacaac ctttgtattt
     1381 ttccgcgcat actggacact gaataaacag caggtggcag ccatcatttt tacagttggt
     1441 atggctgtcg cacggcgcgc cgcactggtg gcaatgcgcg ataatctcat ccgatatacg
     1501 ttcgcccatc cgctcgtcaa aaacaaaatt tttgccaata aaacgcaccg gcaagccctg
     1561 ctcgcgcgcc ttacgggcgt attcaataat tccgccctcg atatgccaga ctttgttgaa
     1621 tccgttatgt ttcatccagg cactggcctt ttcacaacga atgccgccgg tgcagtacat
     1681 gacgattttt ttatctttat gtgcctgcat catctcgact gcttttggca gctgctcacg
     1741 gaaggtatct gccggaattt cgagcgcgtt ttcaaagtgc cccacttcat actcatagtg
     1801 gttacgcatg tcgataaata gtacatcggg atcgtcaagc atggcgttca cttccgccgc
     1861 ttgcagatac tcaccaacat tgctggcatc aaagtgagga tcgtcaatac cgtcggcaac
     1921 aatgcgatcg cgtaccttca tgcgcagtac ccagaaggat ttcccgtcat catccaacgc
     1981 gatattcagg cgtaaaccct ctaaagccgg gtcgaaggca tagagctgcg cgcgaaatgt
     2041 ttcaacattg ctcgcaggta cgctgatttg cgcgttaatg ccctcatgcg ccagatacac
     2101 tcgcccaaaa acattcagcg cggtaaacag ctgatataaa gcgtcacggg tcgccttagg
     2161 atcggcgatg tggaaatact tgtaaaacga aatggtggtt cgcggttcgc tctcagccaa
     2221 cattttggct tttagcgcgt cgttggaaat gcggttgtgt aacactggca tggtgtacgg
     2281 ttcctgcgag atgggaaagt aaaaatccgc ggcatgatat agcaattatc gataattaac
     2341 atccacacat tttacgctac atttgcgcat taaaaattat ttgttattta caagcgcggc
     2401 aatttcgccc agtcttcagc cacaaatttt ggttgcgggc gaaaaaatgc gacaatacat
     2461 acaattgccc gaataggttg aaaaacagga ttgatatgac gaatctaccc aagttctcca
     2521 ccgcactgct tcatccgcgt tattggttaa cctggttggg tattggcgta ctttggttag
     2581 tcgtgcaatt gccctacccg gttatctacc gcctcggttg tggattagga aaactggcgt
     2641 tacgttttat gaaacgacgc gcaaaaattg tgcatcgcaa cctggaactg tgcttcccgg
     2701 aaatgagcga acaagaacgc cgtaaaatgg tggtgaagaa tttcgaatcc gttggcatgg
     2761 gcctgatgga aaccggcatg gcgtggttct ggccggaccg ccgaatcgcc cgctggacgg
     2821 aagtgatcgg catggaacac attcgtgacg tgcaggcgca aaaacgcggc atcctgttag
     2881 ttggcatcca ttttctgaca ctggaactgg gcgcgcggca gtttggtatg caggaaccgg
     2941 ggattggcgt ttaccgcccg aacgataatc cactgattga ctggctacaa acctggggcc
     3001 gtttgcgctc aaataaatcg atgctcgacc gcaaagattt aaaaggcatg attaaagccc
     3061 tgaaaaaagg cgaagtggtc tggtacgcac cggatcatga ttacggcccg cgctcaagcg
     3121 ttttcgtccc gttatttgcc gttgagcagg ctgcgaccac gaccggaacc tggatgctgg
     3181 cacggatgtc cggcgcatgt ctggtgccct tcgttccacg ccgtaagcca gatggcaaag
     3241 ggtatcaatt gattatgctg ccgccagagt gttctccgcc actggatgat gccgaaacta
     3301 ccgccgcgtg gatgaacaaa gtggtcgaaa aatgcatcat gatggcacca gagcagtata
     3361 tgtggttaca ccgtcgcttt aaaacacgcc cggaaggcgt tccttcacgc tattaaatct
     3421 cccctgccgg atgcttcaga atggcatccg gcattaccac agcaaatccc cctgatttag
     3481 cgataaaagc tctctggatt gcgccccctg gaagtcgggc gcataattag tgtgcttatc
     3541 ttttcttctt atgttcaccg cgcctggcgc accaacagcg gattgctatg tcaccctgtg
     3601 aaaatgacac ccctataaac tggaaacgaa acctgatcgt cgcctggcta ggctgttttc
     3661 ttaccggggc cgccttcagt ctggtaatgc ccttcttacc cctctacgtt gagcagcttg
     3721 gcgttaccgg tcactccgcc ctgaatatgt ggtccggtat tgtcttcagc attacatttt
     3781 tattttcggc catcgcctca ccgttttggg gtggactcgc cgaccgtaaa ggccgaaaac
     3841 tcatgctatt acgctctgcc ctcggcatgg gcatcgtgat ggtgttgatg ggactggcac
     3901 aaaatatctg gcagtttttg atcctacggg cgcttcttgg gttacttggc ggatttgtcc
     3961 ccaacgctaa tgctcttatc gccacacaag taccgcgtaa taaaagcggc tgggcgctgg
     4021 gtacgctctc cacaggcggc gttagtggtg cgttgctcgg cccaatggct ggcggcctgc
     4081 tcgccgatag ctacggctta cgtccggtat tctttattac cgccagtgtg ctcatactct
     4141 gctttttcgt caccctgttc tgcatcagag aaaaattcca gccggtcagc aaaaaagaga
     4201 tgctgcacat gcgggaagtg gtgacatcac ttaaaaaccc gaaactggta ctcagcctgt
     4261 ttgtcaccac gttaatcatc caggtggcga caggctcaat tgcccccatt ctgacgctgt
     4321 atgtccgcga actggcgggt aacgtcagta acgttgcctt tatcagtggc atgatcgcct
     4381 cggtgccagg cgtggcggct ctgctaagcg caccacgact cggcaaactt ggcgatcgaa
     4441 tcggacccga aaagatcctg attacagcgc tgatcttttc tgtactgctg ttgatcccaa
     4501 tgtcttacgt tcagacgcca ttgcaacttg ggattttacg ttttttgctc ggtgccgccg
     4561 atggtgcact actccccgcc gtacagacac tgttggttta caactcgagc aaccagatcg
     4621 ccgggcgtat cttcagctat aaccaatcgt ttcgtgatat tggcaacgtt accggaccat
     4681 tgatgggagc agcgatttca gcgaactatg gtttcagagc ggtatttctc gtcaccgctg
     4741 gcgtagtgtt attcaacgca gtctattcat ggaacagtct acgtcgtcgt cgaatacccc
     4801 aggtatcgaa ctgatttttc gcctttcata cttgcaaaag cggagaatca gctatccttt
     4861 tccctgaaac ctcatcaact caaagggaga atcgtgatga ccatgtacgc aacgcttgaa
     4921 gaagccattg acgctgcacg cgaagaattt cttgcagaca accccggcat cgacgccgaa
     4981 gatgcgaatg tgcaacagtt caatgcccaa aaatacgttt tgcaggacgg cgacatcatg
     5041 tggcaagttg agttttttgc cgacgaaggg gaagaaggtg aatgtttacc tatgcttagc
     5101 ggtgaagccg cgcaaagtgt ttttgatggc gactatgatg agatagagat acgccaggag
     5161 tggcaggaag agaatacatt acatgaatgg gacgaggggg aatttcagct tgagccaccg
     5221 ctggataccg aggaaggacg cgcagcagct gatgagtggg atgaacgtta atcactcata
     5281 cgggccatga tgagtgtcga tcggcagcag taaagtatca aaaaccagag agaatggcag
     5341 atcaaggatc gtgacataac gccaggcgga gtcacgcaca tcccattgca caccgggata
     5401 atattggttg ccatgcccct gccccggtat agtgcgacta ataatgctgc cacagccact
     5461 cagcaaggtc accatgatgc tcaccacaat taaacgcatt tgatacctct ttcgtaaaaa
     5521 aataccggca taacgccggt atttttattg gctcgttgtt tttgccggat gcggcgtgaa
     5581 cgccttatcc gacccacaaa atcttgcaaa atcaataaat tgcaggaacg atgtaggcct
     5641 gataagcgta gcgcatcagg caactacgtt tttattgcga agccgcatcc ggtttacgca
     5701 atgccagtgg attgagcttt atcccttcgt aataactcac ccatgaagaa tatctctccg
     5761 gggaattcca gacgcggaaa tgcagacggg ccatcgtcac cggatcgctt agcagcacca
     5821 ggcgacgatc gcgattcagc ttctctggcg tctcgttcag cgcctgttca acgtggcggt
     5881 cacgggcgat ttccagcacc ttactggcgc ggtgacgcgc ggtcgccatt gcggttgcca
     5941 gagcgttaaa tgacgggtta aacactgcgt gcataaagcc atcatcaagg gagcgttgac
     6001 gattcatctc aaggaaccga tcggtatcaa ccagcacctg cggcggcgaa tactcttccg
     6061 ggatcaggaa cagtttccag cgtttggtgc gcagaccaac ggtggcacgg ctggaaatca
     6121 ccgaaacaaa cggtgacagg atcaacgaga agacaatcgg tgccagccag aacaggaaac
     6181 gcagatccag ccacgccatc ccaacagccc acactaaccc taacagcagc tgtgagccgt
     6241 ggcgtttgaa cgcttcaccc caggaagtgg agtcatcatc acgctgcggt gaattccaca
     6301 ccacttccca gccaaggaac gcgctgacaa cgaagaccgt atggaacagc atgcgtaccg
     6361 gagccagcag cacggaaaaa agcacttcca gcagcaacga taatgtaacg cgccagaagc
     6421 cgccgtattc tttcgttcct ttgcaccaga taagcaaaat gctcaataac ttcggcagga
     6481 acaacagcac catagtcgaa gcaaaaagtg caatcgccag ctcaggacgc cactgcggcc
     6541 acaccgggaa caactgccgt ggttgcagga agtattgcgg ttcggtcaac gcatgtacta
     6601 cctgcaatgc agtagagagc gcgaggaaca taaaccacag cggagcggag agataagaca
     6661 tcacgcccgt caggaacacc gcacggtgaa ccgggtgcat acccttcacc aggaacagac
     6721 ggaagttcat caggttaccg tggcaccagc ggcggtcacg ttttagctca tcaagcaagt
     6781 taggcggtaa ttcttcataa gaacccggga gatcgtaagc aatccagacc ccccaacctg
     6841 cacggcgcat caacgccgct tctacaaagt catgtgacag gattgaaccg gcaaaggaac
     6901 cttcgcccgg cagcggagcc agtgcgcagt gctcgataaa cggtttcacg cggataatcg
     6961 cgttatgtcc ccaatagtgc gactcgccaa gttgccagaa gtgcaaaccg gctgtaaaca
     7021 gtggcccata cacgcgggtc gcgaactgct gacagcgcgc atacagcgta tccataccgg
     7081 acgctttcgg cgacgactga atgatcccgg cgttcgggtt ggcttccatc aggcgcacca
     7141 gcccgcacaa acaatcaccg gtcattaccg agtcagcatc cagcaccacc atgtagctgt
     7201 actggctgcc ccagcgacgg cagaagtcat cgatattacc gcttttacgc ttcacgcggc
     7261 gacggcggcg gcgatagaaa atctgacctt cgccaccgac ttcagcgata agctccatcc
     7321 aggctttttg ctctgcgacg cagatatccg ggttataact gtcactaaga atgtagacat
     7381 caaagtgttt ggcattcccg gtggctttta ctgattccca cgttgcacgc aggccagcaa
     7441 aaacacggtt cacgtcttcg ttacagatag gcatgatcaa cgccgtgcga tgctccgggt
     7501 ttaatggttc atcgccaact gttgacgcag atatactgta tttatcgcga ccaataagca
     7561 gttgcaggaa gcccattaac gccgtccaga atccggcgga cacccaacag aacagtaccg
     7621 caaagaggat caggataccg gtttgcagca tataaggcag aagctgcata aaggaaaccc
     7681 acaaatcctg accaaccata tccataggat taatcagcgc ccaaccctga taaggaagaa
     7741 tggtcttcat ataccaggtc gcgacgacag tttgcgcgag cgtcaggatc aacagaatgt
     7801 aacggcggat ggtaccgacg gtacgccact tttgctcgct ctcctgctct tctttggtca
     7861 aacgagccag atagcgcggc gtgacatcgc gtccacgcag gcgatcccag aaacggccta
     7921 ccgggttggt acgccacggg tcgggaaaca tcgaggagcg ttttgcttct ggcatcgcct
     7981 tcagctgatc gcgcccttcg tcgtctttaa ttaactgtcc atcagcaagt gaatctggcc
     8041 aggcttgttc cagacgcgcc tttaccgagc cttgcgggga atcatcctcc cgcgcccagg
     8101 tgcggtgttc ggcatccagc gcctgatgaa cggcgcggat atcagtcttc ggcaatgccg
     8161 ctttctcgct tgcggcgatg ggcattgcgt caatgtactc agttgtctta ttcattggca
     8221 ggtaactggt agctccaggt ttcactcaac gtctgatcgg cattcaccag cgcagcacgc
     8281 atttcagtgg ttttcttggc atctttcact ttcacacgca tcaccagacg ccagcctttg
     8341 gtaaccgggt tatagcgcac cgtgctttca actatctcac cattatcacc aatgctggtt
     8401 tgcgctgtga ccggggtatc ctctggcagt tttttcatct ctgcgccggt aaaatcgacc
     8461 acaaaggcga tagtaccgtc aggctggcga atcaggttcg actgcttcac atcccccgtt
     8521 gaacgacgcg tttgttgcac ccatgcgtta tctggcgcat gcagtttgtc ttcatcacgg
     8581 ctgaaggtga tggtgtattt aaagttcatc tctttacccg gctccggcag ctgatccggc
     8641 gtccagtaag cgacgatgtt atcgttggtt tcatcgttgg ttggaatttc caccagctca
     8701 acgctgcctt taccccactc ccctttcgga gtcacccatg cgcttggacg aagatcgtaa
     8761 cgatcatcga gatcttcaaa gcgggagaaa tcacgaccgc gctgcaacag accgaagcct
     8821 tgcgggttct ccatcgagaa gctgctgacc gctaaatgtt tcgggttatt caacggacgc
     8881 cagatccatt cgccattacc ggcatggata gagagaccgt tagagtcgtg caactccgga
     8941 cgatagttat ttgcaggcga cggttggttc ggcccaaaca ggaacatact ggttaacggt
     9001 gcaaccccca gtttgccgac tttatcgcgc agatagattt tcgactgcac atccacaacc
     9061 gtgtcacgcc ctggcataac tacgaatttg taagcacctg tcgcgcgcgg cgagtcaagc
     9121 aatgcataaa tggttaaacg tttatcagtc ggttttggac gctcgatcca gaactctttg
     9181 aagcgtggaa attcttcacc cgatggcaag gcggtatcaa ttgccaggcc gcgggcagaa
     9241 aggccataaa cctgacctgc accaatcacg cggaaatagc tggccccgag catgctgacg
     9301 atttcatcgt ttttatcttt gctgttgatc gggtaaagca ctttaaaacc ggcaaaacca
     9361 aggtctttta ccgtgtcttt gtcatgctga acatcgccga aagtgaaata atccgggctg
     9421 tatttgattc gtttgactgc ggtggcagtc acttcattta ttttgaccgg ggtatcgaag
     9481 tacataccct gatggtagaa ctcgagtttg aatggggtct tcagattgtt ccagtacgct
     9541 ttgtcatgat taaactggat ctgctgatag tccgcgtatt tcatatcgcg gaaaacggag
     9601 ggcaagttgc ttttgggcgc ctcatagcct ttcccggcta aggattgagc ttgctttgcg
     9661 acatcatcaa tactgaaagc ccagcttgaa gatgtataca gggttaacat tactgcagca
     9721 ctcaaccaac gcattttcat catttgtagt ttatgtttca taattagtaa gcacttcccc
     9781 ctttgtgtgc ttatatcgat ccgatctatt ttaatggata accagccatt ccgacaacag
     9841 aatcccggta tcgttcagca cgctggctca tggattaagc acctgattgc aaccgttttc
     9901 actttccgtg cttctcctaa agacagaagc atcgacttcg tgtagggttt gccagcaatt
     9961 ttatttatca accgtcttac agacgcttcc attctggtta gtggtagcga tgatgctatg
    10021 cataaaaacc ttgaagcctg cgcctggcgc ggtcaggtcg gtgtcgctat ggcaacaagc
    10081 ctaaaggcat aacccgacag ggataagacg aaaaatccga gattagttaa ccatatatga
    10141 atccagtacc cgcgcaacgt gaatatttcc tcgactccat ccgcgcctgg ctgatgttgt
    10201 tagggatccc ttttcatatt tctttaatct attcgagcca tacatggcat gtgaatagcg
    10261 ccgaaccatc attgtggctg acccttttta atgacttcat ccactcgttc cgcatgcagg
    10321 tatttttcgt tatatccggc tacttttcct acatgctttt tttacgctat cccttgaaaa
    10381 aatggtggaa agtacgtgtc gaacgtgtag gtatcccgat gttaacagcc atccccctac
    10441 tgacattacc gcaatttatt atgctgcaat atgtcaaagg aaaagcggaa agttggcctg
    10501 ggctgtcatt gtatgacaaa tataatacgt tggcctggga attaatatca cacctgtggt
    10561 ttttactggt gttagtagtc atgacgacgc tgtgcgtatg gatatttaag cgcatcagaa
    10621 ataatttaga aaattctgat aaaacgaata aaaaattctc gatggtaaaa ctatcggtga
    10681 tttttttatg cctcggcatc ggttatgcgg taataagaag aacgattttt attgtgtatc
    10741 cgcccattct gagtaatggc atgttcaatt ttattgtcat gcaaacgctg ttttatttgc
    10801 cgttctttat cctcggcgca ctggctttca ttttccctca tcttaaagcc ttgtttacca
    10861 cgccgtctcg tggctgtacc cttgcagcag cattggcgtt tgtcgcttat ttactcaacc
    10921 agcgctatgg cagtggcgat gcctggatgt acgaaaccga gtcggtgatc accatggtcc
    10981 tcggtctgtg gatggtgaat gtggtcttct cctttggcca ccgtttgctt aacttccagt
    11041 cagcgcgggt gacttatttt gttaacgcat cgctgtttat ctatctggtt caccacccgt
    11101 taacgctgtt tttcggcgca tacattacac cgcacatcac ctccaactgg cttggttttc
    11161 tctgtggcct gatatttgta gtagggattg cgataattct gtatgaaatt catttgcgca
    11221 tcccgttact gaagtttttg ttctctggta aaccggttgt taagcgtgag aacgataaag
    11281 caccagcccg ttaagccaca tttacaataa ccattccacg ggcaatatcg acgccagtct
    11341 gaccataacc cgcttccaga aactggtggc gggttctttt ttgagaataa tctctttctt
    11401 tgcatgacga tcaacccagt tgatccgtcc ccacctgtcc agacggagct gccaggccgc
    11461 atcatactgg ctctgaataa agcgtttatc aattaactgt gccagcgttt cgctctctat
    11521 cacgaagccc atttcagtat tgagcaatgt tgaacgcgga tcgaaattga aagagccgat
    11581 aaacaccgtt ttaccatcga tgctaaaggt tttagcatgc aggctggctc cggaattacc
    11641 ggttatgccg cgatcgtgta acgtactact ttgttcacgc gtcggcttga gttcatataa
    11701 ttccacgcca tagcggagca attttttgcg ccagcgcgcg tatccggcat ggacgacagc
    11761 aacatcgtta gcggcaagag aattggttag gatcgcaatc tttacccctt ttctcaccat
    11821 ccgtaagagt tgcgccacac ctgcgcgtgt cggtacaaaa taggaagaga taatatcgat
    11881 gcgttcactg ggtgagccca tgatatcgaa catgcgctgc ggtaacagtg aatgccgttt
    11941 tgccttgccc tcccctttcg ccggatcatc acttaataaa cgtgttttcg cccagataag
    12001 cggcaacgtt ccatcaacca gatgatttat aaacggactg gattccattt tgcgtaagta
    12061 acgatgcgtc atagcatcgt tatgccagga ggcgggtaac tcgatgcgat ccgccatttc
    12121 accttccggg acatccagca cttgctgtaa gggtgaaacc gatttgcaat accagtagcg
    12181 ggcgaaatca tcggcaacgt cctctaccac gggccctatt gccataacat ctaaatccga
    12241 aaaaagtggc tcttcccctg ccccaaaata ggcatcacca atatttcgcc cccctaccag
    12301 cgtcaccacg ccatcgacag tgaaactttt attgtgcata cggcgattga gacgggaaaa
    12361 gtcggtgata taaccaagcg gacgcagcaa gcgaaacgag aaaggattaa aaagccggac
    12421 ttcaatgcgg ggatggctgt caagcaagcg taaaatgtcg tcaagtccgg gcgtattgtt
    12481 gtcgtccagc aacaaacgga cgcgaacgcc acgctttgct gcggctaaca gggcggaaaa
    12541 cagtaaccga cccgacatat catcctgcca gatgtaatac tgaacatcca gcgtatgctc
    12601 tgccatttcg gccagacgat accgggcggc aaacgcatcc agacttttct ccagagggaa
    12661 aagaccacac tgaccgggat gttgcgaaca cagtggcagc accgcgctcg ccagccgggg
    12721 caaatcattc atctccttgt tgggtaagga gtctttcgta gaggtgggcg ttttcttcat
    12781 cataacagac aaagtatacc tgttcgggta aagcgtgacg ggtaataaat tctgaaacgg
    12841 ttttaactgc gatttcagcc gctgccgcac gagggtaacc ataaacccca gtactgattg
    12901 caggaaaagc cactgacgta tagctgtttg ccgccaccag tcgcaggcta ttcagatagg
    12961 catcctgcaa aagctggtct tcgttttgtt caccaccacg ccagactggc ccgacggtgt
    13021 gtactacggc tttagcggga agatcgcctg caagcgtaat aacggcatgc cccgtagggc
    13081 aatcgccctg ctgttgcctg acttttaaac aggcatccag cagggccgga cccgctgcgc
    13141 gatgaatggc cccatcgacg ccgccgcctc ccattaatga cggattagcc gcattcacaa
    13201 tcacatcaac ggccagtttg gtaatatcac cctgcacaac atgaatacgc gttttcataa
    13261 cgcctcctta caccttcttg tttcttaagt gtacagcagg ctttttggtt aaatcgcatt
    13321 tttctgctca gcaacgtgcg ttgttcagaa acttagttat attcgatgtt cattacagcc
    13381 agctttttct gtttatcaag atactgcact tttactgagg ctatttgcgc gtttttaaca
    13441 ttaccgctca ttaacgcttt gctggaatag gttttgcccg gaacagaacc attctgtggg
    13501 cagttcattt ctacgggtga tgactgggtg ctgacgtcac atgccggttc cacaatttgg
    13561 ccataaaaat ggatcacccc cccttgcgcg gtgcttcctg cgatggcaat aaaaggaaaa
    13621 aacaaactgc cgagcataag agagtttaaa aaaggtctga acatttattc tatttcctca
    13681 atgattagtc atccttgagg gttgtgttat ccatactttc aggttggctg attcgcagca
    13741 gaccattctc tccagattca tcttatgctc gatatttcaa caaattaaga tttttctgaa
    13801 gagggcggcc attgttgtga taaatgaagt gactgtccat cagaaacagt aacaactatt
    13861 ttcacccgat cgtccgggga aatatttaaa ctcaacttcg tcaaagcaat gggttgatta
    13921 gcaggcaatg aaagggtctt ttcttgcttc gtctgacttt gccctgaact gccttcgcgc
    13981 agggacaata tttgtactct gcacagacaa gattgagtaa gagtgacttc aggaataatg
    14041 gtatacacat ccccttgctg ggtcgtatta aaggttatct ggctggaaag tgccgcaagg
    14101 agtaataacg cattcatatt cttctcccga aaaaaaacag ggcttgcgcc ctgtttctgt
    14161 aatacaaatg atgtattagt actgatgagc ggtcgcgttg ttaccaaagc caacctgagt
    14221 cacgttgacg gaggagttag atgcagtctg gtcaactgca gcaccgttgc caccaccgaa
    14281 ctgtttaacc gtcatttcag aatttttgcc gttccactga tcaagagtag cgctgttacc
    14341 gaagccacgt tgggtcagat cgattgagct gtcatccgag ccctgaccaa catctgcacc
    14401 attaccgccg ccatgctggg taatagtcaa gtcagagtta cgggcatcag tttgcagagc
    14461 aagtgcagag ttaccgccac cgtactggta aatgttcagc tctgaatttg ggccgctatt
    14521 attaccgcca ccaccgtggt taccgccgcc gccgtactga ggaacaacac ctgccagagc
    14581 gctaccggag aatacgattg ctgcaattgc tgctactttt aaaagtttca tgtaaaaccc
    14641 ccatcggatt gatttaaaag tcgaatggaa attaacgttg tgtcacgcga atagccattt
    14701 gcgactgtct ctgtactaca attgccgttt tttgagtacc atactgtgta atatttgctt
    14761 tattaccaga acctttctgg ataatcatcg cagtattacc ataagcacct tgcgaaatac
    14821 tggcatcgtt ggcactgccc gcctgatcaa tatatgcaag gttataatct cctgtctggt
    14881 caatctttgc ccgattgcta ctaccttctt gcgcaacaac cgccaaaagt tttgagcctc
    14941 cctgccgtaa ctgagcacta ttattcgtcc cagcttgacc aattatggct gcctgattaa
    15001 atgaagactt actcaattca tttaccgcga agttatattc tgaattagct aaatcataac
    15061 ctgctgcggc tgcaatccca ggcgcaccca gtattgttaa catcataaat aacaatttgt
    15121 ttttcatgtt gtcaccctgg acctggtcgt acatttaaga aattaaatca tttcaacttg
    15181 gttgttaacg caacctgtat tttgttaacg ctgcgttacg atggaaagta tgtctgcgga
    15241 aatattttta ataactcacc cgcgcgttgt atttttcttt ttttgttcat accaaagtat
    15301 taaaacccac gtaaatacgc tgttatctac gcaaaaaata ttttgttttc ttttaaatct
    15361 ccgttttccg ctatcaaaaa tcaccagaca gtcatttttc ttgcccgtcg ctgattgctg
    15421 cgatatgtct tgcgcacaag ccgtgacaaa ccttgtcttt ttgcagtaaa aaattgtcca
    15481 cggaggtgcg gagaaaaaac aagaacgttt tacatgacga aaggactaca ccgaaatatt
    15541 ttttatatgt attattagta agttatcacc atttgtatga ttttttaaaa ttgtgcaata
    15601 aaaaccaaat gtacaacttt tctatcattt ctaaacttaa taaaacctta agattaacat
    15661 tttaatataa cgagttacat ttagttac
//
LOCUS       DAZGAN020000113        15508 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGAN020000113 DAZGAN020000000
VERSION     DAZGAN020000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.113"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..790
                     /locus_tag="ACHKYO_RS19575"
                     /old_locus_tag="pgaptmp_003915"
     CDS             <1..790
                     /locus_tag="ACHKYO_RS19575"
                     /old_locus_tag="pgaptmp_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HGU4430682.1"
                     /translation="ASPLVQEAASLDSPLNAGEIRELRGRMADNERAISAPVTSVVPR
                     ISSLTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHFDVQFNGN
                     MVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPRQGPA
                     SPVVAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPDTTIWQHGDDLLVRSR
                     AMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            794..2125
                     /gene="traO"
                     /locus_tag="ACHKYO_RS19580"
                     /old_locus_tag="pgaptmp_003916"
     CDS             794..2125
                     /gene="traO"
                     /locus_tag="ACHKYO_RS19580"
                     /old_locus_tag="pgaptmp_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HGU4430683.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     KNQRRDTQPADQAEGSGQPSTPPQADQTAREKQREALQKLLARINTQHPDARPPVVAA
                     AMWGTTGAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTE
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALN
                     EHTGMSSVASDVNHRWFKHIVLPSVLGGVGSVGSLYKDANTQVIQGNYGSITGRVGMP
                     DGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISPPS
                     SGTAPSVPPPSGRPLTQEEAERQASDRIQAAAIRKQNEMRQRYGIQQESHP"
     gene            2122..2835
                     /gene="traP"
                     /locus_tag="ACHKYO_RS19585"
                     /old_locus_tag="pgaptmp_003917"
     CDS             2122..2835
                     /gene="traP"
                     /locus_tag="ACHKYO_RS19585"
                     /old_locus_tag="pgaptmp_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HGU4430684.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCVLIIL
                     VAVWYKFLRTPSEPPADAGFAVTPPAAESWAEPMPMTETGSGNTMSAPPVSPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNGQSTKTPPSRLQGMQIMSVEKGMAWIRWQGSAWAVREGDRLGNIT
                     ITRIEPSSRSVITSAGTLR"
     gene            2832..3362
                     /gene="traQ"
                     /locus_tag="ACHKYO_RS19590"
                     /old_locus_tag="pgaptmp_003918"
     CDS             2832..3362
                     /gene="traQ"
                     /locus_tag="ACHKYO_RS19590"
                     /old_locus_tag="pgaptmp_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HGU4430685.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAA
                     AGAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVAGVLLVF
                     IDRVIDASLASLGLAF"
     gene            3411..3809
                     /locus_tag="ACHKYO_RS19595"
                     /old_locus_tag="pgaptmp_003919"
     CDS             3411..3809
                     /locus_tag="ACHKYO_RS19595"
                     /old_locus_tag="pgaptmp_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HGU4430686.1"
                     /translation="MSRFRAMTARLCAAFIYVQLYLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGLCLAVVPELMGSGQKTLGLSPVSIS"
     gene            3866..4117
                     /locus_tag="ACHKYO_RS19600"
                     /old_locus_tag="pgaptmp_003920"
     CDS             3866..4117
                     /locus_tag="ACHKYO_RS19600"
                     /old_locus_tag="pgaptmp_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HGU4430687.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPGPGTLAFPAPATGHGIQR"
     gene            4032..4850
                     /locus_tag="ACHKYO_RS19605"
                     /old_locus_tag="pgaptmp_003921"
     CDS             4032..4850
                     /locus_tag="ACHKYO_RS19605"
                     /old_locus_tag="pgaptmp_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HGU4430688.1"
                     /translation="MNKTPPLIAPARERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILDDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRIRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRQMLREKMDGMALILLPDSFPDKKYVM"
     gene            4905..5264
                     /locus_tag="ACHKYO_RS19610"
                     /old_locus_tag="pgaptmp_003922"
     CDS             4905..5264
                     /locus_tag="ACHKYO_RS19610"
                     /old_locus_tag="pgaptmp_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430689.1"
                     /translation="MDTLTAGYQFSADAFRHFIIRTLTDDIPPDGVEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHNSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            5264..5644
                     /locus_tag="ACHKYO_RS19615"
                     /old_locus_tag="pgaptmp_003923"
     CDS             5264..5644
                     /locus_tag="ACHKYO_RS19615"
                     /old_locus_tag="pgaptmp_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430690.1"
                     /translation="MKIHRISPETLITLILAHLAGKADSTAKEEHRLLRRFLRDDDGR
                     LAGILLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     QNKRRKSIVVHLNKITYKQTHSIR"
     gene            complement(5617..6162)
                     /locus_tag="ACHKYO_RS19620"
                     /old_locus_tag="pgaptmp_003924"
     CDS             complement(5617..6162)
                     /locus_tag="ACHKYO_RS19620"
                     /old_locus_tag="pgaptmp_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430691.1"
                     /translation="MKAENKILNLFHATTMSRGLQIIKDGKIRTNAPSVICWSLETTP
                     GWIYLSDQLDNAVHWGNKIACLSKDELFFYIFRVQVQEENCYPDTDDLQYVSWLSPEE
                     AENTDLYTCLSLCHSCRTDKELYTGCEISDYAVLPATTLYTDNSGHPQYDIVNTLKKH
                     IGKDEYNQYESLRQRIEWVCL"
     gene            6387..9431
                     /locus_tag="ACHKYO_RS19625"
                     /old_locus_tag="pgaptmp_003925"
     CDS             6387..9431
                     /locus_tag="ACHKYO_RS19625"
                     /old_locus_tag="pgaptmp_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4430692.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNIGHKISFVFERDPERGRNEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHGSGPVPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISNLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHMAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQELGSLRARQI
                     LAENFPRGSATSLIEHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            9431..10048
                     /locus_tag="ACHKYO_RS19630"
                     /old_locus_tag="pgaptmp_003926"
     CDS             9431..10048
                     /locus_tag="ACHKYO_RS19630"
                     /old_locus_tag="pgaptmp_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HGU4430693.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGVRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALMSDNTWLWPDSPGTPALTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHS"
     gene            10009..11214
                     /gene="traW"
                     /locus_tag="ACHKYO_RS19635"
                     /old_locus_tag="pgaptmp_003927"
     CDS             10009..11214
                     /gene="traW"
                     /locus_tag="ACHKYO_RS19635"
                     /old_locus_tag="pgaptmp_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HGU4430694.1"
                     /translation="MQRQPATTLLALLIGCAVIPSTHAYSVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPTREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            11211..11780
                     /gene="traX"
                     /locus_tag="ACHKYO_RS19640"
                     /old_locus_tag="pgaptmp_003928"
     CDS             11211..11780
                     /gene="traX"
                     /locus_tag="ACHKYO_RS19640"
                     /old_locus_tag="pgaptmp_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HGU4430695.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCHHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            11853..14018
                     /locus_tag="ACHKYO_RS19645"
                     /old_locus_tag="pgaptmp_003929"
     CDS             11853..14018
                     /locus_tag="ACHKYO_RS19645"
                     /old_locus_tag="pgaptmp_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HGU4430696.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNNQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSQGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTATSASEYQASNTQNPQSALLEFIGPGLTRFSNKVATIHSGTGTT
                     SQQANPLLKMKDIGDYTMVTTETIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAK
                     AFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALYAAALM
                     NVQINSVTGVFSMAGFLMIYARACTTLVAGVFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNIRRTPGVREDKLKNINRDDKDGIKQ"
     gene            14089..14739
                     /gene="excA"
                     /locus_tag="ACHKYO_RS19650"
                     /old_locus_tag="pgaptmp_003930"
     CDS             14089..14739
                     /gene="excA"
                     /locus_tag="ACHKYO_RS19650"
                     /old_locus_tag="pgaptmp_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HGU4430697.1"
                     /translation="MNKTKSRYPSWWAYYHLARNAYFLLGIPCLLLYSAAYTSDLLDD
                     NLRHSTSLFILAYGCYFLLFIPPLWLYLKTRAKKKKVLQVVQQIKESSPFAPTSDYEQ
                     LSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSL
                     PMIPLEISGISARTLANTIHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            complement(14804..15013)
                     /locus_tag="ACHKYO_RS19655"
                     /old_locus_tag="pgaptmp_003931"
     CDS             complement(14804..15013)
                     /locus_tag="ACHKYO_RS19655"
                     /old_locus_tag="pgaptmp_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HGU4430698.1"
                     /translation="MSMSPAIANTFLLEMMKDKSKDVTLAVIYALGEGRCQADNIKRE
                     LRRLSESDDMEIKVAAIKALGRLYR"
     gene            15108..15239
                     /locus_tag="ACHKYO_RS19660"
                     /old_locus_tag="pgaptmp_003932"
                     /pseudo
     CDS             15108..15239
                     /locus_tag="ACHKYO_RS19660"
                     /old_locus_tag="pgaptmp_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutE"
BASE COUNT         3745 a         4412 c         3977 g         3374 t
ORIGIN      
        1 tgcctctccc cttgttcagg aagccgcatc actggattcc cccctgaatg caggtgaaat
       61 tcgggaactg cggggacgaa tggccgataa cgaacgggcc atcagcgcac ctgtcaccag
      121 tgtggtgccc cgtatcagct ccctcacggt aaatctgtca cccggggcca gtctgccgct
      181 tgtccgaacc gcactgcata acgtcagcgt ggtaacgttc acggacatca acggctcccc
      241 gtggcctcag tcggatccgc cttataacgc ggcacccggt cactttgatg tgcagttcaa
      301 cggcaatatg gtctccgtca ctcccctgcg cgcctgggct gcagggaatg taacggtcta
      361 cctcaaagga ctgtctgtac cggtcattct gaatatcacc agtggtgaaa ccgacaccaa
      421 taccctgagt caggagatcg acagccgtct ggatctgcgc attccccgtc agggacctgc
      481 cagtcctgtg gtagccgtac catcagagaa gattgccctg catgatgcca ctctgcaggc
      541 ctttctggac ggtgttcctc ccgatgatcc caccgtgcgt cggctgaaat tctccggaaa
      601 tgttccggac accaccatct ggcagcacgg cgatgatctg ctcgtgcgat cgcgggccat
      661 gctgcgggat gaatttgaac agacgctggc atctgctgac ggtacgcgtc tgtggaaact
      721 gccggtgacc cccttactca ccttctccgt gaatggtcag tccattcacg tcacccctga
      781 actggagtaa atcatgagcg cagaacagga cacagccgca aagtccggca aaaaactgtc
      841 agccctgctg gttctcgggg gcgtgattat catcgggggc ggatacctgc tgctcacctg
      901 gctttccccc gaaaacagcg catcgctctc gtcagtcaat atcaacagca cgacaacagg
      961 cagcgggcgg accgtggtgg agtcacagca ctaccgggat ttactgcgtg ctgacaatga
     1021 gcgcggggcc gcagaagcat tacgtaataa ccagacgttc atcgccagtc tgcccagggg
     1081 actggatact gatcacggaa agaatcaaag aagggatact cagcctgctg accaggccga
     1141 ggggtctggt caaccatcaa cgccccctca ggcagatcaa acagcccggg aaaaacagcg
     1201 ggaagcgtta caaaaactgc ttgcccgcat caacacacag catcctgacg cccgcccccc
     1261 ggtagtggcg gctgcaatgt ggggcaccac tggtgctccc gggataagct cacatgcctc
     1321 accacccggt accggcacat caccacaacc acgtctgcag gaggccagtc tggcaacccc
     1381 ggcagctcgt acagggatac agctgatacc ggcactgacc cgcgtacctg ccttcattga
     1441 tacggaagtg gactctgaca atgctgtctc ccgcgtggtg gcctccgtac ctgccggccc
     1501 atgggccggg gccgtcctct acagccagga cgccagactc gccggcaagg gaatggatat
     1561 ccactttgac cggatgatgt ggcggggcat gtttctgaaa gtcaatgcat atgccctgaa
     1621 tgaacatacc ggcatgtcat ccgtggccag cgacgtgaac caccgctggt ttaagcatat
     1681 cgttctgccg tctgtgctcg gtggggtggg cagtgtcggg agtctttaca aagacgccaa
     1741 cacacaggtc attcagggaa actacggcag cattaccggc agggtgggaa tgcctgatgg
     1801 cgaggcgctg gctggcgtca ttgcaggcgg catggccgaa cggggcagtc agttgctgac
     1861 ccgccaggcg gagagcgaac cttaccggca ggtgacagtg tcacgcggag aggttgtctc
     1921 cattctgttt gttgagccgg tcatgagcaa cgatatcagt cccccctcct ccggcactgc
     1981 cccttctgtg ccaccgccct ccggacgccc gctcacccag gaagaagcag agcgtcaggc
     2041 gtctgacagg atccaggcag ctgccatacg taaacagaac gaaatgcggc aacgttacgg
     2101 aatacaacag gaaagtcatc catgaaaaac gaacatgaag atcccggcag ttttggcgaa
     2161 acaacgacac cggcatccgg caaatccttc ctgaaacgcc agtatatggg atacagcctg
     2221 ccagtctggg ccggctgtgt cctcatcatc ctggtcgcag tctggtacaa gtttctgcgg
     2281 acaccctcag agccccctgc agatgcaggc ttcgctgtca caccgcccgc agccgaaagc
     2341 tgggcagagc ccatgcccat gacggagacc gggagcggaa acaccatgtc cgcgcctcct
     2401 gtctccccac aggcagacat gcagcagctt gcccgtgacc tgaaagcaga gctggacagg
     2461 cgggatgagg aaatccgacg taccctgacc atgctgaaag agagcatcac aacccttggc
     2521 gacaccatac ggaaagatga ggcctgggca caggaaaccc gtcagttgct tgatgaaacc
     2581 cgtaagcagg tgcatgccct gcagatgcag ggaccactgc aaatcccgac cgggaataac
     2641 ggccagtcaa cgaaaacccc gccttcccgc ctgcagggca tgcaaatcat gtcggtggaa
     2701 aaaggaatgg cctggatccg ctggcagggt agcgcctggg cagtacggga aggtgacagg
     2761 ctggggaata tcaccatcac ccggattgaa ccgtccagcc gctcagtcat cacctctgcc
     2821 ggtacattac gatgatgagc attgatgccc tgacagccat agagaacttt gccagcggta
     2881 tattcagtgc aggcatggag tttctcttca cctggggaga gctgctgggg attatcggcc
     2941 tgattggtca tctgatgcgt gcccgggctg aagggcgtca cagtatggga ccgggaaaat
     3001 ttatcgccgg tatcgtcatc tgcggcatgc tggtcgccct gccatccttc atcaatgccg
     3061 ggggtaccca gatgggattc agggctgatt ccttcggccc tatcgcttat gtacaaccaa
     3121 ccacctttgg tgccgctgcc ggtgccgcca atgccatgct ctcactggta aaactggccg
     3181 gagtggggtt tgccatgaac ggcatttcca tctggcgtaa atccctgctg gacgggcata
     3241 ccgccctgag cagcagtgag tccatcagca aaggaaacgt caagtttgtt gccggcgttc
     3301 tgctggtttt tatcgatcgc gtcatcgatg cctctctggc gagtcttggt ctcgccttct
     3361 gatccacatt cattttcagt aaatcagtct gttagttaag gagacactat atgtcacgat
     3421 tccgtgccat gacagcacgt ctgtgcgctg catttattta tgttcagtta tacctggtgg
     3481 cctgccgtga ccggctgata cgcctgttac ttctgcttcc cgtgatactg ctgccccaca
     3541 cagcgatggc aaacaccctc ttcgatatga tcaaaaccgt tggtgatggt gcaaaaggct
     3601 ccagcagttc atttgtggct atcggtcagg ttattggcct ggtgctcttt atcggcggcc
     3661 tgcttggcct caaacaaatc ggtaaaaaac ccggagcagg gctggcctgc ctgctgtcaa
     3721 ttctggtcgg cctctgcctt gccgtcgtac cggaactgat gggtagcgga cagaaaacgc
     3781 tcggtctttc tccggtttca atcagttaat ccgcaaaggc accgggaaac cggtgccgtc
     3841 ttcacactat gaggaggacc agaccatgtc ctgtgagctg ccccctttca ccctgctcag
     3901 aacagagcac ggcaacgtga ttgcccgcga caaccgggat aatcaggagt ttgtgtttgc
     3961 agagtgttat tccaccgcca tcgcagcact gattctggct gccgtcaccc tgtatacaga
     4021 gagaaaaacg tatgaacaaa acaccgccac tcatcgcccc ggcccgggaa cgctggcttt
     4081 tcccgctccc gcaaccggac acggtattca gcggtgaatc cctgctgcca ccacccgtgc
     4141 tctcaacacc cggacgctgt ccctgctgtc gcaggaccgt cactcaccgc ttcattctgg
     4201 atgattcatg gccattacag cagatggctg acacctgccg ggatacggtc gtcctgctgg
     4261 aaaaaaacct cacccgggtg atgcgcctga aaaaacatcc ggtaccggaa aacgctgacg
     4321 aaaaaaagaa acacacccga accctgcagg atgcggagcg gtccctggca caggcccgcc
     4381 tgtcggccag gcgactggcc ctgcgtcatg tggaaaagtc acaaatcgtc acaacagatg
     4441 cactcagcga aaatgaaagt gaactgctgc agcctgaagg cccgcccttt catctctgtg
     4501 ccttctgcca cgcctggcac tgcctgaacg gctacgctgc cgcacagggg gtgatggtct
     4561 ggctccctga tttgcatccg gcctcggtag ttgctctcaa tgcacgggca ctgaaggaaa
     4621 ttttcagcga tgagcgtaag cgaatcagac agggacgggc cgtactgaat gccctggtac
     4681 agaaccgcct ggccgttgag gagaaatttc gcacctggcg tccggcggat tttgctgatg
     4741 cactgcgccg ctggccgccg gcacagcgtc aaatgttgcg ggagaaaatg gacggaatgg
     4801 cgcttatcct gctgccggat tcgtttcctg acaaaaagta cgtcatgtga cacacaccgc
     4861 tgccggtgac gttctgccgg ccccaactga cagaggaaca aaaaatggat accctcactg
     4921 ccggatatca gttttccgca gatgcattcc ggcactttat tatccggacg ctgacggacg
     4981 atattccgcc agatggagtt gaaaaagaga ttgcccggtt cattctccgc cacccggcat
     5041 ttaacggcgg ctggctgctc tttctggctg cagaaattca tgaaagatcc cgtacccata
     5101 acagcatcac cgtcacagtg agcgtcgata actatctctc atggatggat attgccgtgt
     5161 ccggtctccc ggaattaccg gatatgccct tctgtgattt tttcattcgc cgccagcagt
     5221 ttatttatcc gggtgtaccc ccgacaaaat caggagaaga atgatgaaaa ttcaccgcat
     5281 atccccggag acgcttataa cactcatcct cgcacacctg gccggaaaag ctgacagtac
     5341 cgccaaagaa gagcaccggc tactccgtcg gttccttcgg gatgatgacg gcaggctggc
     5401 cggcatactg ctgaatattg ccggaatact tcagttcaac agggaattaa gcgcccggca
     5461 taattatccg gccacacccc tgactgaatt ttcattaagg aaacggggaa aacaacttca
     5521 tctctgtctc tgcagtctcc gtttctttta tattcctccg gtatttatac aaaacaaaag
     5581 aagaaaatct attgttgtac atcttaataa gatcacttat aaacaaaccc attcaatacg
     5641 ctgacgaagt gattcatact gattatactc atctttgcct atatgttttt taagcgtatt
     5701 aacaatatca tattgaggat gtcctgaatt gtcagtatac agtgttgttg caggaaggac
     5761 tgcataatct gatatttcac aacctgtata cagttctttg tcagttcggc aggaatggca
     5821 aagagaaaga catgtataca ggtctgtgtt ttctgcctcc tcaggagata accacgacac
     5881 atattgcaaa tcatctgtat caggataaca attttcttcc tgaacctgaa ctctgaaaat
     5941 atagaaaaat aattcatctt ttgaaaggca tgctatttta ttcccccaat gcaccgcatt
     6001 atcaagttga tccgataaat aaatccatcc tggtgtcgtc tctaacgacc agcatataac
     6061 agaaggagca ttagttctta ttttcccatc ttttattatt tgcaggcctc tgctcattgt
     6121 tgtcgcatga aaaagattta atattttatt ttcagccttc actctatttc ctcatttccc
     6181 aagaaacata ttaaaaacgg aaaacattac acataacctg gaggacaaat catgacttta
     6241 aatttccctg ccggggaaac tacactattc tcagcaaaaa acatcctgac agcaatactc
     6301 actctgcaca acttacacgc atagcgttcc catttgaata cagttgcagc ccgcgattta
     6361 ttttgtcttc cggagccacc tctgccatga atatcaaccg cattattttt gccattgaag
     6421 actgtatcag taccctgtca cggtattccg tttccgcatt cttccccgat tactgtgatc
     6481 tgcacaccgt catcggtctg gaccagcagg accgcgaacg ccgcccctgg ctgaaagccc
     6541 cctacattgc caccacaaaa caggggtatt acctttccgt ctttgaaatc agcggtgccc
     6601 tgaaagaaat ggatgaaaac ccggacctga cagcccccgg cacactggaa tcactgatta
     6661 cagcgctggc agagcgaatg aacactgcct ataaaaacat cggtcacaaa atcagttttg
     6721 ttttcgaacg cgatccggag cgtggaagga atgaaatcga agccatgctc ttaccccagc
     6781 gaaaatccat cgcacgcacg gggatcctgc ttcaggacgt tctggatgaa aaagtcacca
     6841 cgctcacccc ctggcttgtc cgggaacgct gctggctgac cgtctggagc tctgaagaac
     6901 tgctgagccg gaccgatcgc aaggaccacc agacccgtat caggaaactc gctgaacacg
     6961 ctccgccagc ccgatttgcg caggatccat ggcgctggac actttccgca ctcaaaatac
     7021 ggcatgacgc ctttctggac acgctggagc aggccctgac acatgattct gatggtctgc
     7081 tggttcgcct gatggatatt catgaagtgg gacgggaaat ccgccgtcaa ctggagcgca
     7141 acagcacacc tgcagtatgg cagcctcatc tgcctgaaga tgcccgtcct gccggctggc
     7201 gacagggcga ggatacctct gtattccacg caccgtcgct gaatctgcag ttattcacca
     7261 cacagcctga aacacacggc agcctgataa aggccggtga cctctggcac ggtatggtcg
     7321 ccattaccct gcccccccag aatctgcgga cattcaacca gctggtcagg gatgtgcccc
     7381 gcgcaatccc ctggcgtatc cggatggatc tgatgcccgg cggcatgaag gcgcttgggg
     7441 tcaagaaaac cctgctgacc tacagcagct ttatttcccc tctccggccc atgtatgact
     7501 cagtgatgat gctgaatgac atcaacaaac atgatccggt ctgtatcatg actattgttg
     7561 ccaccacatg gggaaacagc cgggaagtct gtacccgcaa ccaggcgctc ctgaaatctg
     7621 cgctggaagg ctggggagta tgcggcacca ccaccacctt cggtgatccg agacgtgcct
     7681 gggtgaacac ggttctggcg gcctcccacg gttccggtcc agtccccctt tacccgcccc
     7741 tttcacacgc cctttccctg tttcccctga acagggccgg ctctgtctgg cggggacagg
     7801 gaaatctgat gatgcacacc gaagacggct ctgcatggga ggtcgcgctg gcatcgtcac
     7861 aacagaacaa gcacacggaa ctgacgcccg gcgcacccgg tctgggcaaa tcggtactca
     7921 tcaacgcact gtcggagatc cagatagcct cagcccagaa gaacctgcct ttcattgcct
     7981 atatcgacaa gggattcagt gcacagggac tggtacagct tatccgtgac agcctgccgg
     8041 agcagagaaa ggacgaagcc gtggggatca ttctgagcaa tgatccggaa cacacccgta
     8101 acctgtttga tgtgatgtac ggtgctaaaa aacccgccac cccggaaaag aacttcatgg
     8161 tatctgtact ctgcgcactg tgcgtggata ccggcaccgg tcagccctgt aatcccggtg
     8221 acacccggca aatcataagc aacctggtgg atctggcctt cagggagtat ggcgaaaaca
     8281 accccagact gtaccgtgca ggaacagagc cccttgtgga tctcgccctg gaagagagtg
     8341 gcatcgcaga acaacatgac gctggatggt ggaatgctgc cacctggttt gaaatcaggg
     8401 acatgctgca tatggctggc aatatccccg cagcccagcg ggcacactac caggccatgc
     8461 ccctgctggc tgaaatgtcc gccctgctgg gacagccatc catccgggat gtgttcggca
     8521 cagttcagcg cgataactca gcagaattgt tactggacta catccgccgt gccctcgatc
     8581 agggacacag tgactacccg atgatgtccg gctgtacccg gtttatgctc agccccgaca
     8641 cccgcgttgt ggccgttgat ctgaacaatg tggccggtga caagacacct gccgggcgtc
     8701 tgcgcactgg catcatgtac ctgctggcag gacaaatcgc cggcggggat tttgtcctgc
     8761 cgcagtatca ggaggaaata cggaaaaatc tcgacccacg ctaccatgag gtgattttcc
     8821 gccgcattga acagcttgat caggaggtca agaccaaagt ctatgacgaa ctgcacaatg
     8881 caaaagggat caatttcatc tgggaagccc tggacaccca ggagctggag cagcgtaagt
     8941 tcgggatacg cacagtgctg agcacccagc tactgcagga ctatccaccg gcagttctca
     9001 aatccgccaa caccctctgg ttgctgcgtt accgccctga cgaaattccg tttctgcggg
     9061 ataattttgg tgtccctgaa gtcaccctgc ggcgcttcct gaaaatgccg gaaggggctg
     9121 caccggatgg cagcggtgtc ccggtactgg ctgtgttccg tgtaaaaaac ggtacgctgg
     9181 cccggatcct gaaattcact ctcggtccac tggagctgtg ggcactgaat tcctcaccca
     9241 aagacagcgc cctgagacgt gcgctgacgc aggagctcgg gtcactgcgg gcacgccaga
     9301 tcctggcaga aaactttccc cgtggttcag ccacctcact gatagaacac cgcgcccgta
     9361 cacatgacag tgaaaacgtc atccatgatc tggctgcaga actcatccgt aagcagggat
     9421 atcacctctg atgtacagca tacacatcac tccccgctgc cggcgtcctg cacggggaca
     9481 ggtcttcctg aaaacgctgc gcgtggacgg tgacagtttc agcttcaccc tgccgtccgc
     9541 ctgcttcacg ctgcgtccct cccccaatcc ccgggtcatt ttcgccagtg acagacgggc
     9601 tgacggggaa atgagtggcg ttcgcctggc tctgcccgga gagacaggca cgctgattac
     9661 aacggccacc tggcaggtca acgtgccgga gcggcctcct tttgatatac agcatgtcat
     9721 tcactggctc ctgccctcgc ctctcacggc gctgatgtcc gacaacacct ggctgtggcc
     9781 ggatagcccc ggcacaccgg cactgacggc gggagactgg ccggtaatgg aaaccgatac
     9841 cccgacgctg aaacgcgtga agcgtcgtac cttcacgctc atcgataacc gcaaccgtca
     9901 gggatggatc acccgtcgct tccagtgctt cccgctggct ccgctgccgg aatcacacag
     9961 tcaccgcgta ccagacatcc tgtatcccct gaaaacggag aaaaaagaat gcaaagacaa
    10021 ccagcaacca cactgcttgc actcctgata ggctgtgcgg ttatcccgtc cacccacgcc
    10081 tattctgtga ccgtagtggc cagccagcct gtcaccacac aaatcatgcc ggagctggcc
    10141 gccattcagg gcgtgatggc agagatggtc cgtatgcaga cagccaccgg cacagccatc
    10201 aaccagagtt cagagaaact ggcctctgtc attgcgcagg atgggcaggc cacccgtcag
    10261 cagatgatat tcagtaatga aacccgccgg cttgaggaag cccggaagag cttcagcgtg
    10321 ccggactcca tctgcagtga atccgcctcc ggagttgcag ctgaaagtcg ccgtgcagca
    10381 gcgtctgcgg ctgcacgtct gagtcaggga ggaggcgtca gcagtaaacc aatccgtgaa
    10441 cgtctctccc gggcagcaga ctcaccaacc cgcgaggcat atgacagtgc aggcattcat
    10501 gccggttact gcacggaagc tgaatatgcc cgctttggcg gaaccgatgt ctgccctgcc
    10561 gtgggcgagc tgccgggcgg agacagccag gtgcgatcgc tctatcaggg tgcaggcaca
    10621 gcagataccc ctgccgccct cacctgggat cagaaacaga ttgatgctgc cacggcttac
    10681 atgaaaaaca ccgccagacc gtcagcgggt cgtgccccgg ggaaaggtga agtgggaaca
    10741 cagaccgggc gcacgtatgt gggcctgcag aatgaataca acggcatcat cgatgccgct
    10801 tctcatccgc aactgtcact gatagctgac agtaccccta atgaggccac ccgtggagcg
    10861 ctgactgaag ccctgcagtc accatccgct gcggcatatt ttgaccggac cgcatcatca
    10921 gaggcccgca cccgtggtca catgagccag cgggagtttg aggcctttga agctggcaga
    10981 cgctatgcaa acaccgactg gcagcaggat ctgcagggca tggaaggcga taaccttctc
    11041 cgtgaactgc tgcgcactac cgccctgctg aactggcaga tgaacgacct gaaagagcag
    11101 atacgccagg gtaatgttat cgccgggcag cagctggcac tggcggcacg acagtattac
    11161 ggacagcgtc tcggtgaact gtcacaggcc atgagccagg ggagcgtaag atgaaagacg
    11221 acaacagcac cgacaaaaaa aaaccggccc cccgtctccg ccgcatcggt gacatcggga
    11281 taaacctgtt cgcaccactg gcagaaaccc ggtggatgct gcgcagctgc caccatatgc
    11341 tgggccagca taaaacgcgc ctgaagcgcc tcatgcccga ccggcaaccg gagagtccgg
    11401 tctgtatgac ctgggaagaa gccgtcaggg ccagtggcct tacggtcaac gaactggatc
    11461 ggcgtctgcg acgccgcagg atgttctggc ggacaggatt ctggctgatg atgataccgg
    11521 tacttctgct gtgcggcatg gcccttgctg ccacagcgct tccggccatc accctgatac
    11581 gtatactggc ctgcctgtta ctgatgagtg ccattgcagc actgtgtgcc tcaaaaaccc
    11641 tgattgtgtc ataccgcctc tggcaactga atgaacggcg ggtcagcgag gacgaacagg
    11701 gcaccttccc cgattacctg caggcccggc acggctggaa acaggccaca tggctggcca
    11761 ttacccaccg gcagtactga ccgtccggtc tgcaattcct tttcctcatt ccggaggctg
    11821 atgccctgca tcccttcggt tccggagtta tcgtgaaaat catacccaga gcactgagtg
    11881 cgggtctggc gctggctgcc gtacctgcac aggcattcgt cacctatcag gacattgtga
    11941 gcgccgccac caatccggat gacctgtcgc gccaggcact ggtcaccatt tttggcgacg
    12001 tggtcacaaa tcccctgaca gccagcagca acaccattat cggtaacctg ttcatggtgt
    12061 ttaacgcaat tatcgccatt atggggatag tctggttcct gtttattgga atccgccatg
    12121 ttgtccgcac cggccaccag ggcaatgtct ttaacaacgg acgggacgtc gtcggaatgc
    12181 tcagtattgt gagcggtttt atgatgattg tgcccaccgc ttccggctgg agcctggcac
    12241 aactggtcat gctctggggc gcatccatca tgggcattgg ttccgccaat atcatggttc
    12301 agacggctgc agaaaacatc gctgaaggct attcaatgac cgtacagcca gttcatgtct
    12361 ccacccgcac ggcagcacgg ggaatactgg agatggcgct gtgcaaacac gccatgaatg
    12421 caggcctcga tgactttaac cgtactgccc gttcatcaac accccccatg agcgaatcct
    12481 cacggacaga gaacggcagg tataccgtca ccgtttcaaa tggcagtgcc acctgcggga
    12541 gtgtgagtct caccgtgaaa gggaatggca gcaacaacca gtccgcgctg gactattttt
    12601 tcaatccctt cattgaaacg gagtatcagg gagtcctgac ggcacaacgg tctgccatgg
    12661 ataccatgct ccgtgatctg gacagcgcag cacaggaatt tgtgtcagcg taccggaccc
    12721 gacgcgacac gggtaacggc aacctgccgg atattgaaag ccgtattcag aaggctgcca
    12781 accggtatga agaaacggtg cagaaggcgt tgccaacaga taacgcagaa actgagcgca
    12841 gggagcagct gaaatcctac ctgaccacct acggctgggt tgccctgggg gcctggtacc
    12901 agacctttgc caccgccaac cagcgactgg cagaactggc cgaccgtgcc cccactgtga
    12961 gtggagtttc atcgcagggc gaagccggag atactgaatt cctgaaggaa gtaaaaggag
    13021 cttaccaggc acagttgcaa aactcaccat atacacctgc actgggaaca gccacctcag
    13081 ccagtgaata ccaggcatct aatacacaga atcctcaatc agccctgtta gagttcatag
    13141 ggccagggtt aactcgattt tcaaataaag tagcaaccat acattcagga acaggaacaa
    13201 cgagtcagca ggcaaatcct ctgctgaaaa tgaaggacat tggtgattac accatggtca
    13261 caaccgaaac catctgggcg ggttatacca ctgtcagggt gctttcaact gccggcagca
    13321 actccgtttt tggccgactg gtcaatgccg tcagcggctt atctgatgca gcaaaggctt
    13381 ttctggaagc ctccgcacca ccactctact tcctgctgtt tttgctgttc tgtgccggtt
    13441 tttcgttgtc catttacctg ccgtttatcc cgtttatctt ctggatgacc ggcatcggca
    13501 actggatagt cagcgtactg attggatgcg ccgccggccc cctgtgggcc gcaacccacc
    13561 tgggaacaag tcaggacaga ggaagtcggg ccgcttacgg ttacatctac ctgatagaca
    13621 gcatgatcag gcctctgttg atggtgttcg ggttcttttt tgcatcggtc gccatcgtgg
    13681 ctggcggcac catactgaat gctctgtacg ctgcagcact aatgaatgtc cagataaatt
    13741 ctgtgacggg ggtgttcagc atggcgggat ttttgatgat ttatgcccgc gcctgtacca
    13801 cactggttgc gggcgttttt gcacttcagg cttatctgcc agaccatgtg attaattttc
    13861 tgggtggacg tgacggcgtt aatatgctgg ggaatatggt ttcttccgtc aaagatatta
    13921 ttgccggcgg aaacaccaat atacgcagaa cacctggcgt tcgtgaagat aaactgaaaa
    13981 atattaatag agatgataag gacggaataa aacaatagag tacaaaacag tattgccata
    14041 ttcacctgaa gagtttaaaa tatattcagg tgaatatgga ggtttattat gaacaaaacg
    14101 aaaagcagat atccttcctg gtgggcatat taccacttgg cccgcaacgc ttatttctta
    14161 ttaggaatac cgtgtttact tttatactca gccgcttata catcagattt attagatgat
    14221 aatctaagac attcaacaag cctttttatt ttggcatatg gatgttattt tcttcttttt
    14281 attcccccac tctggctata tctgaaaacc agagcgaaga aaaagaaagt cctgcaggtt
    14341 gttcagcaga taaaagaaag cagtcccttt gcgccgacca gcgattacga gcaactgtca
    14401 ttctcgaaga gttgttattt cgggatcgac attaaaaacg gcaccatgct gtatgtgcgc
    14461 atttacccaa acaatgtcat ggatgtgatt ggtctggata ttcacaactt cacccgaaca
    14521 gttgctgaag acggcaagct ggaaatccac accacctatg tgagtctgcc gatgatccct
    14581 cttgaaatct ccggcatctc agcaaggacg ctggccaaca ccatacacac catggctgca
    14641 cgtggatatg agtacaaaga gcgtttcccg cagatgattc ggtatcgggt taaagaatgg
    14701 gaaaaagttg ccggtgtgcc tgttgcagaa gtgttttaat acggctcata catccggttc
    14761 atctgaaccg gattttcttt actacaacag ataacttata tcgttatcga taaagtcgcc
    14821 caagagcctt gatagctgca actttaatct ccatatcatc actttcactt aatctacgta
    14881 attccctctt tatgttatct gcctggcatc gaccttcacc taatgcatag atcacagcaa
    14941 gtgtaacgtc tttcgattta tccttcatca tttcaagcaa gaaagtatta gctatcgcag
    15001 gagacatact cataataatt tcctctttat aaaattaaca aagatactca tttagatgta
    15061 taaacaccgt ataataaaat aattcttgct aaacaagaaa ttaaaatctt gcatccacag
    15121 tctcgcacac catgaagaca ctcggatatg agtacaagga acgtttcccg cagatgatcc
    15181 ggtatcgggt taaagagtgg gaaaaagttg ccggtgtgcc tgttgcagaa gtattttaag
    15241 gtagcgacca actgttagac ggtaatccgt cagccggttt tctctgaacc ggctttttct
    15301 tttcaggcaa aatgcctgtc tgccatccct gtatcagact gtaattagca caatacggca
    15361 atttgccgtt cagtatattc caggaggtac aaatcatgcc atctgcaaca caaactgccc
    15421 gactggaagc aaggatcagc acagaactgc atgctatgct gaaacgcgct gcagaaatcc
    15481 aggtaaaaaa gggcgcattg aggtttgc
//
LOCUS       DAZGAN020000114        15256 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGAN020000114 DAZGAN020000000
VERSION     DAZGAN020000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.114"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            183..1097
                     /gene="sitA"
                     /locus_tag="ACHKYO_RS19665"
                     /old_locus_tag="pgaptmp_003933"
     CDS             183..1097
                     /gene="sitA"
                     /locus_tag="ACHKYO_RS19665"
                     /old_locus_tag="pgaptmp_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HGU4430699.1"
                     /translation="MLSIKKVTMLLGCLVLTCSIAFQASAAEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDAFIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            1097..1924
                     /gene="sitB"
                     /locus_tag="ACHKYO_RS19670"
                     /old_locus_tag="pgaptmp_003934"
     CDS             1097..1924
                     /gene="sitB"
                     /locus_tag="ACHKYO_RS19670"
                     /old_locus_tag="pgaptmp_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HGU4430700.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            1921..2778
                     /gene="sitC"
                     /locus_tag="ACHKYO_RS19675"
                     /old_locus_tag="pgaptmp_003935"
     CDS             1921..2778
                     /gene="sitC"
                     /locus_tag="ACHKYO_RS19675"
                     /old_locus_tag="pgaptmp_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HGU4430701.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQLTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            2775..3632
                     /gene="sitD"
                     /locus_tag="ACHKYO_RS19680"
                     /old_locus_tag="pgaptmp_003936"
     CDS             2775..3632
                     /gene="sitD"
                     /locus_tag="ACHKYO_RS19680"
                     /old_locus_tag="pgaptmp_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HGU4430702.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENA
                     EPRDLLSSD"
     gene            complement(3866..4123)
                     /locus_tag="ACHKYO_RS19685"
                     /old_locus_tag="pgaptmp_003937"
                     /pseudo
     CDS             complement(3866..4123)
                     /locus_tag="ACHKYO_RS19685"
                     /old_locus_tag="pgaptmp_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            4121..4534
                     /locus_tag="ACHKYO_RS19690"
                     /old_locus_tag="pgaptmp_003938"
                     /pseudo
     CDS             4121..4534
                     /locus_tag="ACHKYO_RS19690"
                     /old_locus_tag="pgaptmp_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            4875..5255
                     /gene="crcB"
                     /locus_tag="ACHKYO_RS19695"
                     /old_locus_tag="pgaptmp_003939"
     CDS             4875..5255
                     /gene="crcB"
                     /locus_tag="ACHKYO_RS19695"
                     /old_locus_tag="pgaptmp_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGU4430703.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLA
                     GLIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTS
                     VLVHVIGSLIMTALGFFIITILFA"
     gene            complement(5635..6828)
                     /locus_tag="ACHKYO_RS19700"
                     /old_locus_tag="pgaptmp_003940"
     CDS             complement(5635..6828)
                     /locus_tag="ACHKYO_RS19700"
                     /old_locus_tag="pgaptmp_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4430704.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLM
                     ATGAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVV
                     LRPQRLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWH
                     YRTIK"
     gene            6964..8688
                     /gene="iucA"
                     /locus_tag="ACHKYO_RS19705"
                     /old_locus_tag="pgaptmp_003941"
     CDS             6964..8688
                     /gene="iucA"
                     /locus_tag="ACHKYO_RS19705"
                     /old_locus_tag="pgaptmp_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HGU4430705.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLM
                     ARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            8689..9636
                     /gene="iucB"
                     /locus_tag="ACHKYO_RS19710"
                     /old_locus_tag="pgaptmp_003942"
     CDS             8689..9636
                     /gene="iucB"
                     /locus_tag="ACHKYO_RS19710"
                     /old_locus_tag="pgaptmp_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HGU4430706.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            9636..11378
                     /gene="iucC"
                     /locus_tag="ACHKYO_RS19715"
                     /old_locus_tag="pgaptmp_003943"
     CDS             9636..11378
                     /gene="iucC"
                     /locus_tag="ACHKYO_RS19715"
                     /old_locus_tag="pgaptmp_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HGU4430707.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            11375..12708
                     /gene="iucD"
                     /locus_tag="ACHKYO_RS19720"
                     /old_locus_tag="pgaptmp_003944"
                     /pseudo
     CDS             11375..12708
                     /gene="iucD"
                     /locus_tag="ACHKYO_RS19720"
                     /old_locus_tag="pgaptmp_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016528830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
     gene            12734..14935
                     /gene="iutA"
                     /locus_tag="ACHKYO_RS19725"
                     /old_locus_tag="pgaptmp_003945"
     CDS             12734..14935
                     /gene="iutA"
                     /locus_tag="ACHKYO_RS19725"
                     /old_locus_tag="pgaptmp_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HGU4430708.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         3657 a         3848 c         3920 g         3831 t
ORIGIN      
        1 tttaaagcca aagcgctgcc caacttttat cctcaatacc acatcgtcag caggaaataa
       61 cctaatccct gtgttgggat cttataatcg ctaatgataa ttatttacat tttatagctt
      121 ctgctataaa acatagtagt tgctatgttt attctaattt tgctcactat aggtactaaa
      181 tcatgctctc aataaaaaaa gtaaccatgc tcttggggtg cctagtcctc acctgctcga
      241 tagcatttca ggcgagtgca gctgaaaaat tcaaggtcat tacaactttt accatcatcg
      301 cagatatggc caaaaacgtg gctggagatg ctgcagaagt ctcatccata accaagcctg
      361 gtgcagaaat tcatgagtat cagcctaccc ctggcgatat taaacgtgcg cagggggcac
      421 aactgattct cgccaatggt atgaatctgg aattgtggtt ccaacgcttt taccagcatc
      481 tcaatggggt tccagaagta attgtctctt cgggtgtgac gccagtaggg atcaccgaag
      541 ggccctatga gggcaaacct aacccccatg cctggatgtc gccagataat gctctgattt
      601 acgtcgataa tattcgtgat gcgtttataa aatacgatcc ggcaaatgca caaacctacc
      661 aacgtaatgc cgatacttat aaagccaaga ttacccaaac ccttgccccc ctgcgtaagc
      721 agattgcgga actccctgag aatcagcgat ggatggtcac cagtgaaggg gctttttctt
      781 atctcgcacg cgatttgggg ctaaaagagc tttatctgtg gccgattaat gccgatcaac
      841 aaggaacacc gcagcaggta cgtaaggttg ttgatatagt taagaaaaat catatcccgg
      901 cagtctttag cgagagtacg atttccgata aaccagcgcg tcaggttgcg cgtgaaaccg
      961 gcgcgcacta cggtggtgtg ctctatgtcg attccctgag cacagaaaac ggcccggtac
     1021 ctacgtatat cgaccttctg aaggttacta ccagtacgct ggtacaggga attaaagccg
     1081 gaaaaaggga gaaataatga tgcaatctgc aggcattgtc gttaatgatg ttactgtcac
     1141 ctggcgtaac gggcacacag cactgcgcga tgcatccttc acagtaccag gcggatctat
     1201 tgccgctctg gttggggtaa acggttccgg aaaatcgacg ttgtttaaag cgattatggg
     1261 atttgtgcgt ctgaccagcg ggaagatatc tgttctgggt attcccacac gacaggcgct
     1321 acagaaaaac ctggttgcct acgtccctca gtcagaagag gttgactggt catttcctgt
     1381 actggtggaa gatgtggtga tgatgggccg ctatgggcat atgggtatgt tgcgtatcgc
     1441 caaaaagagg gatcgtcaga ttgtcaccga tgcgctggaa cgtgtcgata tggtggattt
     1501 tcgccatcga caaatcggcg agctttccgg tggacaaaag aaacgcgtct ttctggcgag
     1561 agcgattgca cagcagggtg atgtgatcct gctggatgaa ccttttaccg gcgttgatgt
     1621 gaaaaccgaa gcgaaaatca tcagcctgtt gagggagtta cgcgcggaag gcaaaacgat
     1681 gcttgtctca acgcacaatc tcggatccgt cacgacattt tgcgattata cggtcatggt
     1741 caaaggcacc gtcctggcaa gcgggccgac agacaccact tttacagccg agaacctgga
     1801 gctggctttt agcggcgtac tgcgccatgt cacacttaac ggctcggaag aaagcattat
     1861 tactgacgat gaacgccctt ttgtggcaca tcgaccgtca gcggtgcaga gggaagaaag
     1921 atgaacgtgc tactggaacc cttcagctat gaatatatgc tcaatgcgat gtgggtctcg
     1981 gcgatggtcg gcggtctctg cgctttcctg tcatgctatc tgatgctaaa aggctggtcg
     2041 ctgattggcg atgcgctgtc gcactctatt gtcccgggtg ttgcgggggc atatatgctg
     2101 gggcttccgt tttctctggg agccttcttt tccggcggac tggcagcggg cagcatgctg
     2161 tttcttaacc aactcactcg cttaaaagaa gatgccatca tcggcctgat cttctcgtcc
     2221 ttttttggcc tggggctgtt tatggtgtca ttgaacccga catccgtgaa cattcagacc
     2281 atcgtcctgg gcaatattct ggcaatcgat ccggcggata tcctgcaact cactatcatt
     2341 ggcattctct cgatcatcgt gctttttttc aaatggaaag atcttatggt gacctttttc
     2401 gacgagaatc acgcccgtgc tattggattg catccgggca gattaaaaat cctcttcttc
     2461 accctgctgt cggtttcgac cgttgcggcg ctgcaaacgg ttggtgcctt tctggtgatc
     2521 tgtctggtgg tgactcccgg tgcaacagca tggttgttaa ccgatcgttt tccccgcctg
     2581 ctcatgattg ctgtaactat tggcagtgtg accagtttcc tcggcgcgtg ggtcagttac
     2641 tttctggacg gcgcgacagg tggaatcatc gtggtggcgc agacactgct gtttctttta
     2701 gcatttgtct ttgcgcccac ccatgggctt ttggcaaatc gtcggcgtgc gcataaagcg
     2761 ctggaggatc gctcatgatg gcattattac tggagccact gcagttcacc tttatgagtc
     2821 acgcactgct gatttcactg gttgtttcca taccctgtgc gctgctgtcg gtctttctgg
     2881 tcctgaaaag ttgggcactg atgggtgacg cgatgagtca tgcggttttt cctggcatcg
     2941 tgcttgcctg gattctgggg ttacctctgg ctacaggggc attcgtcgcc ggagtattct
     3001 gtgcggtggc gacgggatac ctgaaagaca acagccgaat caagcaagat acagtgatgg
     3061 ggattgtttt ttctggcatg tttgccgcag gtctgatctt gtatattgca gtcaaaccag
     3121 acgtacatct tgatcatatt cttttcggcg acatgctcgg gataaccatc ggcgatataa
     3181 tccagacggt gattattgcc ggactggtta cacttgttat tagcgtaaaa tggcgcgatt
     3241 ttttgctgtt cagctttgat tatcaacagg cgcaggcaag cggtttgcat acgagatggc
     3301 tgcattatgg gctgctgtgc atggtctccc tgaccattgt ggcgacgctg aaagcggtag
     3361 gtatcattct ctctatttct cttctcattg cgcctggtgc cattgcggta ctcctgacac
     3421 aacgctttca tattgcgctc ctgctggcga ctggcatatc gatattggtc tcaatgactg
     3481 gtgtctggct gtcctttttt atcgacagcg cgccagctcc gacaattgtt gtcttattcg
     3541 cggtcatgtt catcatgacg tttaccgtta ccagcatcaa cgcacgcaca aaggaaaatg
     3601 ccgaaccacg ggatttgtta tcatccgatt agcagaacac tcggccagca acatccgctt
     3661 attggcgatg cgcttgaacg aataatgcac agacttatga ttctgataag ttttgttgcc
     3721 agagagttgc tgcggactgg atagcaagta atgtcgattc tcaggggtag caaacagcgc
     3781 agtaaaatga gggaagtagt ggcaaaacat agagaaaatt ggggggcgag ggccagtgat
     3841 gtaaaaaacc acgctaagag tttaattaat tgtttgtatt gaagtttaat gatcttaact
     3901 cacccttaag aatatcttcc ggtaacgaag acgtataatg ccccagcata tttatatggc
     3961 tatgtatcag cagcgataat cttgccggat gctactcctc aggagtttct cccgatttgc
     4021 gaagatgtga caacgcctcc tgcatgtaaa gtgtgtttca cagcacaacc gcattggtca
     4081 ccagccccag agcacctagt tgatcttcct gcccttaacg gtagctgaag atgactgggc
     4141 tggatggaaa atcctgcatg gtgcattggg agagcagata gaattggttg gcgacgatct
     4201 gtttgtgacc aacgtaaagt atattcagcg cggcattgac gaaaaccttg ccaattcagc
     4261 attgattaaa ctcaaccaga ttggttccct gagtgaaacc tttgatgcgg tacaactttg
     4321 ccatgacaac aactggggca cctttatctc acaccgcagt ggtgagacag tggacagctt
     4381 tatcgccgat atgacagttg caatgcgcgc aggtcatctc aaaacgggag ctccatgccg
     4441 gggtgagcgt attgaaaaat ataaccagct tatgagaatt gaggatgaac tgggttcatc
     4501 ggcgcaattt gccggaaagt ctgcctttaa atgacattat tgtgccaatc ccccaggctt
     4561 acacgtcggg gttattctgg aggaatgatg aaagctcacg tttttgttat ttctatgctt
     4621 actggcattg tcgtcaccta tgccgtgtta cttcttggtt gcttgttcat cgataaaacg
     4681 ctccccactg tagatgttgt cattctgtca cttgtcgtgg gggcttctgc tcagcaattg
     4741 tcgagggtat taatgtcaat aaatagaacc ttcccttatc ttgcatgtag tcaaaggata
     4801 taaattaaca atatcagtca aatgagaaca tattctgata ttgttacgat aaaaaattat
     4861 tttatgaggg gtacatgatt aaatcacttt ttgccgtcat tatcggcggt tctgttggat
     4921 gtactctgcg ctggttactg tccacacgct ttaacagtct ttttccaaat ctacctccag
     4981 gtacactggt ggtgaacctc cttgccgggt taattattgg tactgcgttg gcgtactttt
     5041 tacgacaacc acaccttgat cctttttgga aattaatgat aacaacggga ctttgtggag
     5101 gtctgagtac tttctctaca ttttctgttg aagtattcgc attactccag gcgggaaatt
     5161 atatatgggc tttgacgtct gtacttgttc acgtcattgg ctccctcatc atgacagctt
     5221 taggcttttt tatcataacc atacttttcg catgagaatt cattaacgtt acaggtttat
     5281 caactaatgt gacgcttagg atagaatttc gctccatggt atgcgtttgc agtcattaca
     5341 gtctccatga cgactgtaat gactgcaaac gcattacctg acatatcagt ataaacaatg
     5401 aattaacctc ttggcgtatt ttttacatca ctggccctca ccccccaaaa ttctctctga
     5461 aaaattgttg cggttaacgt tgccaatgta tccacccatt tcagaagacg atctggcatc
     5521 ctggtggcat gctgcgatgt gcttcaggtg agtgcactgg gcggtcagca caggcggttt
     5581 cgacagcgaa attgcacagg acgttatgcc agctgtgagt acaacatcat agccttactt
     5641 gattgtacgg taatgccaaa tacatgcaga cactatggta aataatacag cggcaaataa
     5701 tgccgtcaca atcagcgcga ttgagacacc tgttttcgac gccagtggca ccaacaatgc
     5761 gcacagccca tagccaagtg tgtgactggc cgcaagccag cccgcgcctg cgccgtcggc
     5821 cagcttatca ttcagtaaaa gttgataagc gggtgttgcc agagcggccc caaatgacag
     5881 cactgcacag cccaggtaaa acaaccatag ctgttcagtg agcatgatag ccagaccact
     5941 actcatcaac accccggcac tcaggagcag agccactgga gtcaggcgct gtggacggag
     6001 taccacgaac tgcgcgataa gcgcagctat tgcggacagc cccaacaacc acgccacctg
     6061 ttggctaata gtggtggtat cagtatcgaa ctggcgagta agggctggcg aaagtccaag
     6121 ctgcatcatg ctcattgccg cagccagtaa cattgcgcaa agcagataag gcaggcaatc
     6181 ccgcttcagg ctgacgctct tgcgctccgg tgtgggctgt ggtggtgtgc cgggcaaccg
     6241 cagaagcatc accacagccc acaccggagc tgccatcagc atccacactg gcgccagagg
     6301 gtgaatgacc aacattgccg ccgcgcacag cggcccgaat agcctgccgc agctcaggcc
     6361 ggagctgatg gttgccagag cggccatgcg attcccttct cccgctctct gcaatgccca
     6421 gacctgacag gctggcacca ttgctgacac ggtcagaccg tagacgatcc gggcgatgat
     6481 taatattccc aaccccaccg ctgttgttac ggcaccggta gccatcagca cgcttcccag
     6541 cccaagcagg gtaaagctta cgccatatcc tcccagagcc cacagcacca cagtcttact
     6601 gccagtaagg gaaatttgct ttccccacca tggggaagat ggcagaaaca gcattgagcc
     6661 cagcatcagc agggcagccc atacagacag actcagattt gtctgtatga ccagaacagg
     6721 gagtacaacc agcagaccgt tctgtccgat accgagaaga ccggcactga acgccagtgg
     6781 ccagcaggac agggtttttt ggggcgtatc ttcgatctta gatgacattg taatgtttca
     6841 acaccatgta ttaattgtgt ttatttgtaa aattaatttt tctgacaata acatttctca
     6901 ttgataatga gaatcattat tgacataatt gttattattt tactgtgtag gagctgtttg
     6961 attatgatcc tgccctctga aaaatccgcc acagatgtgg ctgcgcagtg cttcctgaat
     7021 gccttgattc gtgaaaccaa agactggcaa ctggcggaat acccgccaga cgaattgatt
     7081 atcccgctgg acgaacagaa gtcgctccat ttcagagtgg cttatttctc cccaacccag
     7141 catcaccgct ttgcatttcc tgctcatctg gtcacggcat caggcagtta tcctgtcgac
     7201 tttaccactc tctcccggct tattattgat aagctccggc atcaactttt tctgccagtt
     7261 cccctctgcg aaactttcca ccaacgcgtg ctggaaagct acgcccatac gcaacagaca
     7321 attgatgccc gccatgactg ggccatcctg cgtgaaaaag cgttgaattt tggcgaggct
     7381 gagcaggcac tgctgacagg acacgctttc caccctgcac ctaagtctca tgaaccgttt
     7441 aaccgccagg aagctgaacg atacctgcct gacatggcac ctcacttccc gctgcggtgg
     7501 ttttcggtgg ataaaacgca aatcgctggt gaaagtctgc atcttaacct tcaacagcgg
     7561 ttgacgcgat ttgccgcaga aaatgcgccc cagttactca acgaattaag tgacaatcaa
     7621 tggctgttcc cgctgcaccc gtggcaggga gaatatcttt tgcagcaagt gtggtgccag
     7681 gcactttttg ctaaaggact tatcagagac ttaggcgagg ccggcacgtc gtggctgccg
     7741 accacctctt cccgctccct ctactgtgct accagccgcg atatgatcaa gttctccctg
     7801 agcgttcggc tgaccaactc cgtccgtact ctgtctgtga aagaggtgga gcgaggaatg
     7861 cgcctggcac gtctggcgca aaccgacggc tggcagatgc tacaggcccg cttccctact
     7921 ttccgggtaa tgcaggagga cggctgggcc gggctgcgcg atcttaacgg caacatcatg
     7981 caggaaagtt tgttttccct gcgtgaaaac ctgctgctgg agcagccgca aagccagacc
     8041 aacgttctgg tttccctgac tcaggccgca ccggatggcg gtgattcgct gctggtttcg
     8101 gcggtaaaac gcctgagcga tcgcctcggt atcactgtgc aacaggccgc ccacgcatgg
     8161 gtcgatgctt actgtcagca ggtgctgaag cctctgttta cggctgaagc ggattacggc
     8221 ctggtgctgc tggcgcatca gcaaaatatt cttgtgcaga tgcttgggga tctgccggtc
     8281 ggatttattt accgtgactg ccagggcagc gcgtttatgc ctcacgcgac agagtggctc
     8341 gataccattg acgaggcgca ggcggaaaat attttcaccc gtgagcagtt gctgcgctat
     8401 ttcccttatt acctgctggt taactccact tttgccgtaa ctgctgcgct gggtgctgcc
     8461 gggctggaca gcgaagcgaa tctgatggct cgtgtacgaa cattgctggc tgaagtgcgt
     8521 gaccaggtga ctcataaaac ctgtctcaac tatgttctgg aaagtccgta ctggaacgta
     8581 aaaggtaact ttttctgtta tctgaacgat cataacgaga acaccatcgt tgacccttcg
     8641 gtgatctatt ttgatttcgc taacccgctg caggctcagg aggtctgaat gtctggggca
     8701 aacattgttc acagcggata tggactgcgc tgtgaaaaac tcgacaagcc tctgaatctt
     8761 ggctgggggc tggacaatag cgcggtgttg cactggcccg gggagctgcc aacagggtgg
     8821 ctgtgcgacg cgctggatca gatatttatc gccgcaccac aactttcagc agtggttctt
     8881 ccctggtccg aatggtgtga ggagccacag gcgctgacgc ttttcggaca ggtacaaagc
     8941 gacattatcc atcgttccgc tttctggcag ttaccgttat ggctgagttc tccggcaaac
     9001 cgggcctccg gtgaaatggt ttttgatgca gagcgtgaga tttatttccc gcagcgcccc
     9061 ccccgtccgc agggtgaagt ttatcgtcgt tacgatccac gcattcgcag gatgctgagt
     9121 ttccgcattg ccgatcccgt ttctgatgca gaacgtttca ctcgctggat gaacgatccg
     9181 cgcgttgagt atttctggga gcaaagtggc tcactggagg tacagatcgc ctatctggaa
     9241 cgccagctca ccagtaaaca tgcattcccg ctgattggtt gtttcgacga tcggccgttc
     9301 agttatttcg aaatctactg ggcggcggaa gaccgcattg gtcgccacta ttcgtggcag
     9361 ccctttgacc gtggcctgca tctgctggtt ggtgaacagc aatggcgcgg agcccactat
     9421 gtgcaaagct ggctgcgcgg ggtgacacat tacctgctgc tgaatgagcc acgtacgcag
     9481 cgcacagtac tggagccacg caccgataac cagcgcctgt tccgccatct tgagcctgcg
     9541 ggataccgga caattaaaga gttcgacttc ccacataaac gctcgcgcat ggtgatggcg
     9601 gatcgtcatc acttcttcac ggaggtcggt ctgtaatgaa tcacaaagac tgggatttgg
     9661 tcaaccgccg cctggtggca aaaatgttgt ctgagctgga gtatgagcag gttttccacg
     9721 cggaatctca gggcgatgac cgctactgca ttaacctgcc gggagcacaa tggcgcttca
     9781 tcgctgaacg tggtatctgg ggctggctct ggattgatgc tcaaactctg cgctgcgcgg
     9841 acgagccagt actggctcag acgctgctga tgcagctaaa gcaggtactg tcaatgagcg
     9901 atgcaaccgt tgctgagcat atgcaggatt tgtatgccac gctgctgggc gacctgcaac
     9961 tactgaaagc ccgtcgcggg ctgagcgcca gtgacctgat taatcttaat gccgaccgcc
    10021 tgcaatgcct gctgagcggt catcctaaat tcgtttttaa taaaggtcgc cgtggctggg
    10081 gtaaagaggc gctggaacga tatgcgccag agtatgccaa caccttcaga ctgcactggc
    10141 tggcggtaaa acgtgaacat atgatctggc gctgtgataa cgagatggat attcatcagt
    10201 tgttgacggc cgcaatggat ccgcaggagt ttgcccgctt cagtcaggtc tggcaggaaa
    10261 acggactgga tcataactgg ctgccgctgc cggtacatcc gtggcagtgg cagcaaaaaa
    10321 tcgctaccga cttcatcgct gattttgccg aaggcaggat ggtgtctctc ggcgagtttg
    10381 gcgaccagtg gctcgcccag cagtcgctgc gtaccctgac caacgccagt cgccggggcg
    10441 ggctggatat caagctgccg ctgaccatct acaacacctc atgctaccgg gggatccctg
    10501 gcagatacat cgctgccgga ccactggctt cacgctggct acaacaggtt tttgcgaccg
    10561 acgccaccct agtgcaaagc ggcgcagtga tacttggtga accggctgca ggctatgtct
    10621 cccatgaagg ctatgccgcg cttgcccggg ctccctatcg ctaccaggaa atgcttggtg
    10681 ttatctggcg ggagaatccg tgccgctggc tgaaaccgga tgaaagtccg gttctgatgg
    10741 caacactgat ggagtgcgac gaaaacaatc agccgctggc aggcgcatat atagaccgct
    10801 ccgggctgga cgctgaaacc tggcttacgc aactgttccg ggtggtcgtg gttcctctgt
    10861 atcacctgct ttgccgctac ggtgtcgcgc ttattgcaca tggacaaaat ataactctcg
    10921 ccatgaaaga gggggttcca cagcgtgttc tgctgaaaga cttccagggc gatatgcgac
    10981 tggtgaaaga agagttcccc gaaatggact ctttgcctca ggaggttcgt gatgttacat
    11041 cccgcctgag tgcggactac ttaatccatg atttgcagac gggtcacttc gtgacagtac
    11101 tgcgttttat ttcgccactg atggttcgtc ttggcgtacc tgaaaggcga ttttatcaac
    11161 tgctggcagc agtgttgagt gattacatga aaaaacatcc acaaatgtca gagcgttttg
    11221 cgcttttctc acttttcagg ccacaaatca ttcgcgtggt gctgaacccg gtaaaactga
    11281 cctggcctga tctggatggc ggcagccgca tgctgccgaa ttaccttgag gatctgcaaa
    11341 atccgctgtg gctggtaact caggaatatg aatcatgaaa aaaagtgtcg attttattgg
    11401 tgtagggaca gggccattta atctcagcat tgctgcgttg tcacatcaga tcgaagaact
    11461 ggactgtctc ttctttgatg aacatcctca tttttcctgg catccgggta tgctggtacc
    11521 ggattgtcat atgcagaccg tctttctgaa agatctggtc agtgctgttg cacctacaaa
    11581 tccctacagt tttgttaact atctggtgaa gcacaaaaag ttctatcgct tccttacaag
    11641 cagactacgt acagtatccc gtgaagagtt ttctgactac ctccgctggg ctgctgaaga
    11701 tatgaataac ctgtatttca gtcataccgt tgaaaacatt gatttcgata aaaaacgtcg
    11761 attgtttctg gtgcaaacca gccagggaga atattttgcc cgcaatatct gccttggtac
    11821 aggaaaacaa ccttatttac caccctgtgt gaagcatatg acacaatcct gtttccatgc
    11881 cagtgaaatg aatcttcgtc ggccggacct tagtggaaaa cggataaccg tggttggtgg
    11941 aggacagagt ggtgcagacc tgttccttaa tgcattacgc ggggaatggg gagaagcggc
    12001 ggaaataaac tgggtctccc ggcgtaataa ttttaacgca ctggatgagg ctgcttttgc
    12061 tgatgagtat tttacacctg aatatatttc aggcttctcc ggactggagg aagatattcg
    12121 ccatcagtta ctggatgagc agaaaatgac atcggatggc atcactgccg attctttact
    12181 gaccatttat cgtgagttgt accaccgttt tgaagttctg agaaaaccaa gaaatatccg
    12241 tctgctaccc agccgctcgg taacaactct ggaaagtagt ggtcctggct ggaagttgct
    12301 gatggagcat catctggatc agggcaggga gagcctggaa agtgatgtgg tgattttcgc
    12361 cacaggttac cgttctgcgt tgccacaaat acttccctca ctgatgcccc tgatcaccat
    12421 gcacgataag aacaccttta aagtgcgtga tgacttcact ctggaatgga gtggcccgaa
    12481 agagaacaac atcttcgtgg tcaacgccag tatgcaaacc catggcatcg ccgaacccca
    12541 gctcagcctg atggcatgga gatctgcacg tattcttaat cgcgtaatgg gacgtgattt
    12601 attcgatctc agtatgccgc ccgccctgat tcagtggcgc agcggcacct agggaaaacg
    12661 cagccggagg ctgcttcttt aactcgctac acagcatctt tgggctgatt ttttccgccc
    12721 gtatggagga ataatgatga taagcaaaaa gtatacgctt tgggctctca acccactgct
    12781 tcttaccatg atggcgccag cagtcgctca acaaaccgat gatgaaacgt tcgtggtgtc
    12841 tgccaaccgc agcaatcgca ccgtagcgga gatggcgcaa accacctggg ttatcgaaaa
    12901 cgccgaactg gaacagcaga ttcagggcgg caaagagctt aaagacgcac tggctcagct
    12961 gatccctggc cttgacgtca gcagccggag ccgcaccaac tacggtatga atgtgcgtgg
    13021 ccgcccgctg gtcgtgctgg ttgacggcgt gcgtctcaac tcttcacgta ccgacagccg
    13081 acaactggac tctatagatc cttttaatat cgaccatatt gaagtgatct ccggtgcgac
    13141 gtccctgtac ggcggcggca gtaccggtgg cctgatcaac atcgtgacca aaaaaggcca
    13201 gccggaaacc atgatggagt ttgaggctgg caccaaaagt ggctttagca gcagtaaaga
    13261 tcacgatgaa cgcattgccg gagctgtctc cggcggaaat gagcatatct ccggacgtct
    13321 ttccgtggca tatcagaaat ttggcggctg gtttgacggt aacggcgatg ccaccttgct
    13381 tgataacacc cagaccggcc tgcagtactc cgatcggctg gacatcatgg gaactggtac
    13441 gctgaacatc gatgaatccc ggcagcttca gttgatcaca cagtactata aaagccaggg
    13501 cgacgacgat tacgggctta atctcgggaa aggcttctct gccatcagag ggaccagcac
    13561 gccattcgtc agtaacgggc tgaattccga ccgtattccc ggcactgagc ggcatttgat
    13621 cagcctgcag tactctgaca gcgcttttct gggacaggag ctggtcggtc aggtttacta
    13681 ccgcgatgag tcgttgcgat tctacccgtt cccgacggta aatgcgaaca aacaggtgac
    13741 ggctttctct tcgtcacagc aggacaccga ccagtacggc atgaaactga ctctgaacag
    13801 caaaccgatg gacggctggc aaatcacctg ggggctggat gctgatcatg agcgctttac
    13861 ctccaaccag atgttcttcg acctggctca ggcaagcgct tccggagggc tgaacaacaa
    13921 gaagatttac accaccgggc gctatccgtc gtatgacatc accaacctgg cggccttcct
    13981 gcaatcaggc tatgacatca ataatctctt taccctcaac ggtggcgtac gctatcagta
    14041 cactgaaaac aagattgatg atttcatcgg ctacgcgcag caacggcaga ttgccgccgg
    14101 gaaggctaca tccgccgacg ccattcctgg cggctcagtc gattacgaca acttcctgtt
    14161 caacgccggt ctgctgatgc acatcaccga acgccagcag gcatggctca acttctccca
    14221 gggcgtggag ctgccggacc cgggtaaata ctatggtcgc ggcatctatg gtgctgcagt
    14281 gaacggccat cttcctctaa caaagagtgt gaacgtcagc gacagcaagc tggaaggcgt
    14341 gaaagtcgat tcttatgagc tgggctggcg ctttactggc aataatctgc gtacccaaat
    14401 cgcggcctac tattcgattt ctgataagag cgtggtggcg aataaagatc tgaccatcag
    14461 cgtggtggac gacaaacgcc gtatttacgg cgtggaaggt gcggtggact acctgattcc
    14521 tgatactgac tggagtaccg gagtgaactt caacgtgctg aaaactgagt cgaaagtgaa
    14581 cggtacctgg cagaaatacg atgtgaagac agcaagccca tcaaaagcga cagcctacat
    14641 tggctgggca ccggacccgt ggagtctgcg cgtgcagagc accacctcct ttgacgtgag
    14701 cgacgcgcag ggctacaagg tcgatggcta taccaccgtg gatctgctcg gcagttatca
    14761 gcttccggtg ggtacactca gcttcagcat tgaaaacctc ttcgaccgtg actacaccac
    14821 tgtctggggg cagcgtgcac cactgtacta cagcccgggt tacggcccag cgtcactgta
    14881 cgactacaaa ggcaggggcc gaacctttgg tctgaactac tctgtgctgt tctgaccggt
    14941 attcctttac aacaaaggta cgctgatatc aacatggccg ctgacagcca agttgatatc
    15001 atataataca cgacataatc tgtagtcagg gaggatagac tctttactga ctacagatta
    15061 tgtcgtgttc cgtgctcatt tcctcaaaaa aatacaagaa aagaattagt attctaacaa
    15121 aaagtgaaat aaattgtatc aaactccctc ttttaatcct gttgagtaaa tcagcttttg
    15181 caataggatt gaaagagtgt aagtggaatc tcttccggat actcgttacc accgtggcta
    15241 gaatatctac ggctgc
//
LOCUS       DAZGAN020000115        14973 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGAN020000115 DAZGAN020000000
VERSION     DAZGAN020000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.115"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(143..373)
                     /locus_tag="ACHKYO_RS19730"
                     /old_locus_tag="pgaptmp_003946"
     CDS             complement(143..373)
                     /locus_tag="ACHKYO_RS19730"
                     /old_locus_tag="pgaptmp_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4430709.1"
                     /translation="MARNEVKPDWHPQRILGEVRAREGSMRALARKAGVGQNNLYNAV
                     YRHVPKYEGIIAECLGVPPEEIWPSRYIRKVA"
     gene            559..1119
                     /locus_tag="ACHKYO_RS19735"
                     /old_locus_tag="pgaptmp_003947"
     CDS             559..1119
                     /locus_tag="ACHKYO_RS19735"
                     /old_locus_tag="pgaptmp_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4430710.1"
                     /translation="MIEENGNFSFDAPGKESFKARLLSLIGTRSVRAAAKDWGVPVST
                     LNNYIHKGTEPSFKMACLISNKEHVSLNWLAFGSDENTPSTPCCSTVDAGNLDNANQT
                     ITAILSSLSTDEQLELARLLGRKGADFLTILLDDNIQELHALSGFRRELALRLKDMSD
                     SEAREIYEECEAKVNHLNVTQKKASA"
     gene            complement(1496..1855)
                     /gene="frdD"
                     /locus_tag="ACHKYO_RS19740"
                     /old_locus_tag="pgaptmp_003948"
     CDS             complement(1496..1855)
                     /gene="frdD"
                     /locus_tag="ACHKYO_RS19740"
                     /old_locus_tag="pgaptmp_003948"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU4430711.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(1866..2261)
                     /gene="frdC"
                     /locus_tag="ACHKYO_RS19745"
                     /old_locus_tag="pgaptmp_003949"
     CDS             complement(1866..2261)
                     /gene="frdC"
                     /locus_tag="ACHKYO_RS19745"
                     /old_locus_tag="pgaptmp_003949"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGU4430712.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(2272..3006)
                     /gene="frdB"
                     /locus_tag="ACHKYO_RS19750"
                     /old_locus_tag="pgaptmp_003950"
     CDS             complement(2272..3006)
                     /gene="frdB"
                     /locus_tag="ACHKYO_RS19750"
                     /old_locus_tag="pgaptmp_003950"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGU4430713.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(2999..4807)
                     /gene="frdA"
                     /locus_tag="ACHKYO_RS19755"
                     /old_locus_tag="pgaptmp_003951"
     CDS             complement(2999..4807)
                     /gene="frdA"
                     /locus_tag="ACHKYO_RS19755"
                     /old_locus_tag="pgaptmp_003951"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGU4430714.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            5132..6109
                     /gene="epmA"
                     /locus_tag="ACHKYO_RS19760"
                     /old_locus_tag="pgaptmp_003952"
     CDS             5132..6109
                     /gene="epmA"
                     /locus_tag="ACHKYO_RS19760"
                     /old_locus_tag="pgaptmp_003952"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGU4430715.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            6328..7830
                     /gene="yjeM"
                     /locus_tag="ACHKYO_RS19765"
                     /old_locus_tag="pgaptmp_003953"
     CDS             6328..7830
                     /gene="yjeM"
                     /locus_tag="ACHKYO_RS19765"
                     /old_locus_tag="pgaptmp_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HGU4430716.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVSI
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            7882..8196
                     /gene="yjeN"
                     /locus_tag="ACHKYO_RS19770"
                     /old_locus_tag="pgaptmp_003954"
     CDS             7882..8196
                     /gene="yjeN"
                     /locus_tag="ACHKYO_RS19770"
                     /old_locus_tag="pgaptmp_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HGU4430717.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLLDIYCAYLIRCLVFRGELVGYGDLFHMRYSEIKRPV
                     TL"
     gene            8193..8507
                     /locus_tag="ACHKYO_RS19775"
                     /old_locus_tag="pgaptmp_003955"
     CDS             8193..8507
                     /locus_tag="ACHKYO_RS19775"
                     /old_locus_tag="pgaptmp_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HGU4430718.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDIRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(8538..11861)
                     /gene="mscM"
                     /locus_tag="ACHKYO_RS19780"
                     /old_locus_tag="pgaptmp_003956"
     CDS             complement(8538..11861)
                     /gene="mscM"
                     /locus_tag="ACHKYO_RS19780"
                     /old_locus_tag="pgaptmp_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HGU4430719.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(11883..12851)
                     /gene="asd"
                     /locus_tag="ACHKYO_RS19785"
                     /old_locus_tag="pgaptmp_003957"
     CDS             complement(11883..12851)
                     /gene="asd"
                     /locus_tag="ACHKYO_RS19785"
                     /old_locus_tag="pgaptmp_003957"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HGU4430720.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMANLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(12948..14000)
                     /gene="rsgA"
                     /locus_tag="ACHKYO_RS19790"
                     /old_locus_tag="pgaptmp_003958"
     CDS             complement(12948..14000)
                     /gene="rsgA"
                     /locus_tag="ACHKYO_RS19790"
                     /old_locus_tag="pgaptmp_003958"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HGU4430721.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            14095..14640
                     /gene="orn"
                     /locus_tag="ACHKYO_RS19795"
                     /old_locus_tag="pgaptmp_003959"
     CDS             14095..14640
                     /gene="orn"
                     /locus_tag="ACHKYO_RS19795"
                     /old_locus_tag="pgaptmp_003959"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HGU4430722.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            14851..14926
                     /locus_tag="ACHKYO_RS19800"
                     /old_locus_tag="pgaptmp_003960"
     tRNA            14851..14926
                     /locus_tag="ACHKYO_RS19800"
                     /old_locus_tag="pgaptmp_003960"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14884..14886,aa:Gly,seq:gcc)
BASE COUNT         3480 a         3813 c         3918 g         3762 t
ORIGIN      
        1 ctgtattcaa aacctttagt gccttcctgg cggcgtctgc tggtttcgct ggttgcatac
       61 ttcctgattg aatagcggac gccttgctcc gttcccggca ttcccggtaa tccaaccagc
      121 tctttaactg aagcaaacat aattacgcga ccttacgaat gtagcggctg ggccaaatct
      181 cctcgggtgg aacacccagg cattccgcaa taatcccctc atacttggga acatgacggt
      241 ataccgcgtt gtacaggttg ttctgaccta caccagcttt acgggccaga gccctcatac
      301 ttccttctct ggctctgact tcccccaaga ttctttgagg atgccaatca ggcttaactt
      361 catttctcgc catgattcat ctatcctaaa aagttacgaa atttgataac taaattttgt
      421 tatcaagttt gatacaagta ttgatccaaa tatagtccat gtaaagcaaa aatagctttt
      481 tcttttgtct ccgtgggctg aagtgggttt tatttggtta tctcttttta tttcaatgtg
      541 ttatgaggaa aagaaaaaat gattgaagag aatggaaatt tttcttttga tgcaccagga
      601 aaagaaagtt ttaaagcccg cttactaagc ctgatcggga cgcggagtgt aagggcagca
      661 gcaaaggatt ggggggttcc tgtatcaacc ttaaataact acatacacaa aggcacagaa
      721 ccctctttta aaatggcttg ccttatatca aataaagagc atgtgagttt aaattggtta
      781 gcttttggtt ctgatgaaaa taccccaagc actccatgtt gttcaactgt cgatgcaggt
      841 aatttagata atgcaaatca aacaattacg gcaattctgt cctctctcag caccgatgag
      901 caactagaat tagcccgatt gcttggcaga aagggggctg attttctaac gatcttattg
      961 gatgataata ttcaggaatt acatgcactt agcggttttc gccgagagct tgctttaagg
     1021 ttaaaggata tgtcagattc tgaagcaaga gagatttatg aagaatgtga agctaaggtt
     1081 aatcatttaa atgtaaccca gaaaaaggct agtgcgtaag ggagtggtgc aaattttgtg
     1141 ctgtggtaca ctcctttaaa gacaatctgg agaaaagcgt tttaagcctt tttaaaacca
     1201 ttttaagcgt caatccgtag tatcaaaagt gttgccgatt ttagaataaa cgactaatta
     1261 ttgcacaatc ggtatcaaat agactttccg cgctcgcatt ggtatcacct gttttatttc
     1321 agtatcttac aggtgatttc acctcctttc actaaaaccc tcgttggtat caaatgattc
     1381 acctggttac aagaggcggt aattaataag gcgcagagcg tcgttttgaa cataaggtct
     1441 ggtttccata caaaacggcc cgccataggc gggccggatt tacattggcg atacgttaga
     1501 ttgtaacgat accaatcagc gtgacaactg tcaggatagc agccagaccg tagaaaaccc
     1561 atttgcccgc aggcacgtga attttcagat cgtgcatcgc gtggtgcata cggtgtaaac
     1621 cacaccacag cggcagaacg atcatcagga acaggaatac gcgaccaatg aagctctgcg
     1681 cgaacgccag aacgcgctcg tagctcagcg catcacccgg aaacagcccc agtggcagca
     1741 gaatacccac cagcaggatc atcaccggcg caataattgc gctccacata ccaccggccc
     1801 cgaagaggcc ccagaatacc ggttcgtcag aacgctttgg atttggatta atcatctcag
     1861 gctccttacc agtacagggc aacaaacagg attacgatgg tggcaaccac agttaccgcc
     1921 cagagacttt tgataattgg ctctggtccc attttttcgt cttttacaat gatattggcc
     1981 gctttcggtg ccagttcaaa ccaggttttg gtgtgcagca gagctgccgc cagagtgatc
     2041 aggttaatga tcacgataac cgggttttgt aaaaagtcga cgaatcccgc ccaggcttcc
     2101 gggccatttt tcagggcaaa cagcccgaaa atcagttcaa tgctgaacca cacagccgga
     2161 accgccgtgc cttcgcgcag catgtaaaag cgataaaacg gcaatttttt ccaccaggtg
     2221 gacgtcattg gccgtacata cggtttacgt ttagtcgtca tgttgcactc cttagcgtgg
     2281 tttcagggtc gcgataagaa agtctttcga actttctact ttgccctgct gaatggccgc
     2341 agccggatcg acgtgtttcg ggcagacttc ggagcaatag cccacgaaag tacagctcca
     2401 tacgccgttc tggctgttca actgcgccat acgctccttc ttaccgtggt cgcggctatc
     2461 ttcgttataa cgatgcgcca gcgtaatggc agccggaccg atgaactctg ggttcaggcc
     2521 aaactgcggg cacgcggcgt agcacagacc acagttgatg caaccggaga actggtgata
     2581 ctttgccatc tgcgccgggg tctggatgtt agtaccctga tccgcggtgc gggagttgcc
     2641 gatgatgtac ggtttgatcg cttccagact ttcgatgaag tgggtcatat cgaccaccag
     2701 atcgcgttca atcgggaagt tagctaacgc ttcaaccttc ataccgtcgg tgtaatcacg
     2761 caggaaggtt ttacatgcca gttttggcac gttgttaacc atcatgccgc aggaaccaca
     2821 aatcgccata cggcaggacc agcggtaact caggtccggt gccaggttgt ctttgatgta
     2881 gcccagcgca tccagtaatg aggtagttgc gtcataaggc acttcataga atgcgctatg
     2941 cggtgcggta tcgacttccg ggttatagcg caccacctca attttcaggt ttttcatctc
     3001 agccattcgc cttctccttc ttattggctg cttccgcctt atcggctgca tccgcttcgc
     3061 caccgtaaac gcgtttagct ggcggcagcg tagtaatctt cacgtcgctg tactccaggc
     3121 gagtcgtgcc atcagcatcg cggaaggcga gggtgtgttt gaggaagttg acgtcgtcac
     3181 gctcggtgca accttcgtcc agacgctggt gtgcgccgcg ggactcttta cgtgccattg
     3241 cggagtgcgc catacattca gcaacgttca gaccgtggcc cagttcaatg gtgtagagca
     3301 ggtcggtgtt gaacacgctg gaggtgtcgg tgatgcgcac gcgcttgaag cgttcctgca
     3361 gctctgccag cttgtcgatg gttttctgca tcagttccgg cgtacggtag ataccgcaac
     3421 cttcttccat tgccaggccc atttcgtcgc ggatcttcgc ccagttttcg ccgccatcct
     3481 ggttaaccag atctttcaga cgttgttcaa cgccagctgc ctgcgcttca attgccgctt
     3541 cgttgccatt accggcagtt gctgcacgct ctgtcgcttg ttcaccggcc agacggccga
     3601 agaccaccag ttccgccagg gagttagaac ccagacggtt tgcaccgtgc agaccaacag
     3661 aggaacattc acccacggcg aacagacctt taatgcgggt ttcacagttc tgatcggttt
     3721 cgataccgcc catggtgtag tgtgcggtcg gacgtaccgg aatcggttct ttaaccggat
     3781 cgacgccaac gtacgctttc gccagttcgc agatgaacgg cagacgttca tgcagttttt
     3841 tctcgccgag gtgacgcagg tcgagataaa ccacatcgcc acgcggcgtg gagatggtgt
     3901 tgcctttacg ccattcgtgc cagaaggcct gagaaacttt gtcgcgtgga cccagttcca
     3961 tatatttgtt tttcggctcg cccagcggag tttccgggcc catgccgtaa tcttgcagat
     4021 aacggtagcc atttttgttg accagaatac cgccttcacc gcggcagcct tcggtcatca
     4081 ggataccgga acctggcaga ccggttgggt gatactgaac gaattccatg tcacgcagcg
     4141 gaacgccgtg gcttagcgcc atacccatac cgtcaccggt aacgatgccg ccgttggtgt
     4201 tgtaacgata aacgcgaccc gcaccgccag tagccataac gaccgcgtta gcacggatct
     4261 gcaccagcgt gccttccatc atgttcattg ctaccaggcc gcgaacatga ccatcatcaa
     4321 ccagaatatc cagcacgaaa tgttcgtcaa aacgctggat ctgcgggaat tgcagagagg
     4381 tctggaacag cgtgtgcagc atatggaagc cggtcttatc ggcggcgaac caagtgcgct
     4441 cgattttcat gccgccgaag cgacgtacgt tgacgctacc atccgggcga cggctccatg
     4501 ggcaccccca cagttccagt tgggtcattt cggttgggca gtggtggacg aaataatcca
     4561 cgacatcctg ctcacacaac cagtcgccac ccgctactgt atcgtgaaag tgatattcga
     4621 agctgtcatg atcctgcgcg acagcggcgg agcccccttc tgcagcaacg gtatggctac
     4681 gcatcgggta tacttttgag attagtgcga tttttgcatt tggatttgcc tgcgcggcag
     4741 caattgcagc acgtaatccc gcgccaccgg cgcctacaat ggcaagatcg gcttgaaagg
     4801 tttgcacgac attcctccag attgttttta tcccacagcc ccgtacttca gggtaagtgc
     4861 ctgaaagtta cggtctgcga acgctattcc actgctcctt tataggtaca acagtatagt
     4921 ctgatggata agtctgaaat ttgacgagat cgattttttt agtgcgcaag agggctaaat
     4981 tatcactgaa gatgattaat ttaattacta aaccatcaga tcgtgctttt tttagtcact
     5041 gcccaccgct gtttgattcc tgcgtaaaat ttgtctcgcc gtcgcgttgc gagtagactt
     5101 cgtgcccttg tcaaaaactg gagatttaac tatgagcgaa acggcatcct ggcagccgag
     5161 cgcatccatt cctaacttat taaaacgcgc ggcgattatg gcggagatcc gtcgtttctt
     5221 tgccgatcgt ggagtgctgg aggtggagac gccttgtatg agccaggcga cggtaaccga
     5281 tattcatttg gtcccgtttg agacacgttt cgttggcccc gggcattcgc aggggatgaa
     5341 tctctggtta atgaccagcc cggaatacca tatgaaacgc ctgctggttg ccggttgtgg
     5401 gccggtattc cagctgtgcc gcagcttccg taatgaagag atggggcgtt atcacaaccc
     5461 tgagttcact atgctggagt ggtatcgacc gcactatgat atgtaccggt tgatgaacga
     5521 ggtggacgat ctcttacaac aggtgctgga ctgcccggca gcagaaagcc tttcttatca
     5581 acaagctttc ttgcgttatc tggaaattga cccgctctct gccgacaaaa cgcaactgcg
     5641 ggaagtcgca gcgaaactgg atttgagcaa tgttgctgat accgaagaag accgcgacac
     5701 gctgctacaa ttgctgttta cctttggcgt agagccaaat attggcaaag aaaaaccgac
     5761 ctttgtgtac cactttccag ccagccaggc atcactggcg caaatcagta ccgaagatca
     5821 tcgggtcgct gaacgctttg aggtttatta taaaggtatt gagctggcga atggtttcca
     5881 tgaattgacg gatgcccgtg agcagcaaca acgctttgaa caagataacc gtaagcgcgc
     5941 ggcgcgcggt ttgccgcagc accccattga ccagaatctg attgaagcct tgaaagtcgg
     6001 tatgcctgac tgttccggcg tggcattagg tgttgatcgt ctggtgatgt tggcgctggg
     6061 cgcggagaca ctggctgaag tcatcgcctt tagcgttgac cgggcataat tctgaaaaat
     6121 tactgaatta acagcgaaga atggcgtgat aagcgccatt ctgtagcata tgttttcatt
     6181 tttccgttaa aggccacaat ttaagactgg atatttgata tcatccaggt atcaattctg
     6241 tattggtttt tacgctgcct gctcaatatt gcgcagtgca gttcagtggg cacgtgttat
     6301 acacgcgctg aaatgaagga tggtttcatg cctcacacga taaaaaagat gagtctgata
     6361 ggactcatat tgatgatctt tacttccgta tttggatttg ccaatagccc atcggcttat
     6421 tacttaatgg gttatagtgc gattcccttt tatatatttt ctgcattgtt attctttatt
     6481 ccattcgcct taatgatggc tgaaatggga gctgcttatc gcaaagaaga aggcggtatc
     6541 tattcctgga tgaataatag tgtcggacca cgttttgcct tcattggtac gtttatgtgg
     6601 ttttcctctt atatcatctg gatggtgagt atctccgcga aagtttgggt accgttctca
     6661 acattcctct atggtagcga catgacccag cactggcgta ttgctggact ggagcctacg
     6721 caggtggttg gtctgctggc agtggcatgg atgattctgg tcaccgtcgt tgcttcaaag
     6781 gggattaata aaattgcccg cattactgcg gtgggcggta ttgcagtaat gtgtctgaat
     6841 ttagtattgc tgttagtaag cattactatt ttgttattaa atggtgggca tttcgcgcag
     6901 gatattaatt tccttgcatc accgaaccca ggttatcagt ccggtctggc aatgctatcg
     6961 tttgtggtat ttgctatttt tgcctatggc ggaattgaag cggttggtgg tctggtcgat
     7021 aaaacggaaa atccagaaaa gaactttgcc aaaggtattg tttttgccgc tattgttatt
     7081 tcaatcggtt attcgctggc aatattttta tggggtgtca gcacaaactg gcagcaggta
     7141 ttaagtaatg gttccgttaa cctcggcaat attacctatg tgctgatgaa gagcctcggg
     7201 gtgacgctgg gtaacgcact gcatttgtca cctgaagcgt cattgtcgct gggtgtatgg
     7261 tttgcgcgta ttaccggact ttcgatgttc ctcgcttata ccggggcgtt ctttacgctt
     7321 tgctattcac cgctgaaagc catcatccag gggacgccga aagcgttgtg gccggaaccg
     7381 atgacgcgcc tgaatgcgat ggggatgcct tctatcgcca tgtggatgca gtgcgggttg
     7441 gttactgtct tcatcctgct ggtttcgttt ggtggcggta ccgcatcggc gttctttaac
     7501 aagctgacgc tgatggcgaa cgtgtctatg acgcttcctt acctgttcct cgcgctggct
     7561 ttcccgttct ttaaagcacg tcaggatctc gacagaccgt ttgtgatttt caaaacgcat
     7621 ttgtcggcaa tgattgcgac agtggttgtc gtactggtgg tgacatttgc gaacgtcttc
     7681 accatcattc aacctgtggt tgaagccgga gactgggaca gcacattgtg gatgattggc
     7741 ggccctgtct tcttctcgct gttagcgatg gcgatttacc agaactattg cagcagagtg
     7801 gcaaaaaatc cgcagtgggc ggtggaataa cccgatgccc tatcgtctgc ctgacgatag
     7861 ggcgcaatga caggaaatgt tatggatgac accagccggg acccggcaat aaccgaggat
     7921 gaaatcaggg cgctgcaatt ttctgccggg gatgtagcgg aaatagagca gactattctc
     7981 tcttttgtcg atgcctgcca cacccgtaaa gtggcgatgg tggtgggtag caccattaac
     8041 accctcaaag atcgcgatgg taaacggtgg ggaaacctgc tggatattta ttgcgcttat
     8101 cttatccgat gtctggtttt tcgcggggaa ttagtgggat acggcgattt gtttcatatg
     8161 cgctatagcg aaattaagcg accggttacc ttatgagtgc gcgcatgttt gtcttgtgct
     8221 gcatctggtt cattgtggcg ttcctctgga taaccattac ctccgcgctg gacaaagagt
     8281 ggatgattga tggacgcggg attaacaacg tctgcgatgt gttgatgtat ctcgaagaag
     8341 acgatatccg cgatgtcggt gtgataatga cgttaccgct ctttttccca tttctctggt
     8401 tcgcgctgtg gcgaaaaaaa cgcggctggt ttatgtacgc caccgcgctg gctattttcg
     8461 gctactggct gtggcagttt tttctgcgct atcagttttg tttgtgagcc ggatggggtc
     8521 atccggccac aaagacctta caaacttccc gcctgacgac ctttgcccgc agaagttaat
     8581 gttctccccg tttgcttacc attcaggctt tccagacgca tctggaacgg tgggaatggc
     8641 atgtcgatac cgtgggcgtg gaagcctgcc agaattaact ggtggatctc atggcgcagc
     8701 ggcatacggt gacccatctc agcggcgtaa atacgcagct cgaaaatctg aatcccctgt
     8761 tgcagatcca ccaggaagac ttccggtgcc gggttgtcga tcaccagcga gcagcgacgc
     8821 gctgcggtga gcaggatttc cgtcacttct tcactgttgg catcggcagg ggccggaatc
     8881 gtcaacacca cgcgcgtaac cgagtcagag agcgaccagt tgataaactg ctcggtaata
     8941 aacgccttgt tcggcacgat tatctcttta cggtcccagt cgctgatggt ggtggcgcgg
     9001 gtgttaattt tcgtcacgct accggtgaga tcgcgaattg tcaccgtatc gccaatgcga
     9061 atcggttttt cgaacaggat aatcagacca gagataaagt tggcgaaaat ttcctgcaaa
     9121 ccaaaaccga gaccaacacc gagcgcggcg accagccact gcaatttcga ccactcaata
     9181 ccaatcatcg agaagccgac cagcccgcca atcagcatca gcagatattt ggtgatggtg
     9241 gtgatggcgt agcccgtacc cggcgttaaa tccaggtgct gcaaaatcgc cagttccagc
     9301 agcgcgggca ggttgcgcac cagctgcgtg gtgatgataa acaccagaat ggcaatcagc
     9361 accgcaccga gggtaattgg ctccagactt tctacgccct gtaccgtgga ggtgacatcc
     9421 cacagcgaaa tattttcgag gaagccgaaa gcggaatgga tttctgacca cagcacaatg
     9481 acagaaagca gggcgatcaa catcaaaatt gagcgcacca gccgcaagga ttgcgcactg
     9541 atggcatcga gatcgacttc gctttcatcg acttcaattg ccccttccgg gctactgtga
     9601 tgatgcgctt cctcttcgcc acgcgcgcgt tgcgctaaca tctctgcccg gcgatgcttc
     9661 gcccggtcaa acgccagcct gcgacgctgg atcagcatcc agcggcggat aacgtgataa
     9721 accaccagta gcaggaacca gatggcaacc gaggtttcaa gcctcgctaa cagtgcctgc
     9781 gccgttgcca gataacccac cgctgacgcc agaatggcaa ccagtggtgc gccaatcatc
     9841 atgttccaca gcatatggtt ggtaatgttg tcgccgctgc cctctttgtt gaggtacagc
     9901 gggatcccgg cctttttcag gctgagggtg accaccgcca gcgcaccgca aatgaggata
     9961 aagcaaagcc gtcccagcga accggagaat tcacggtcgt cgaggttatc gaacatcatc
    10021 agcgccataa tcagcggcac aataagcccg atgctcatca ggtagtagcg catcccacgg
    10081 gaaacacgtt cgcgcggcca gccaaaatga gcgataaaca agccgttcgg gcgggcaaag
    10141 gtggcgcaaa tcatcactac ccacagcagc ggcacggtgg ccgttacgcc atcgccaatc
    10201 gcgaccgcca gcggataagg ccacgcctcg cgcaagccgt aacccagcgt catccacagc
    10261 accggcagcg gtgacgcgac gagaatcgac cagaaaagag tgcgcaacgt cagccagaag
    10321 tgatcctgag tcactttgcc gactttcgcc gccgaacgtt caagaaaacg ggtgaaatag
    10381 cggcgcgagt aaatactgca accgaccaga atcaacgcgc caaacagcgg caaaatcgtc
    10441 tctttgctgg tcagcatcat cacactggct ttgcccaact gactgaaggt gtccagcgaa
    10501 atgagacgac gcagatcctg ggcgatttcc agcggccagg cgatggtcat cgggcgcacg
    10561 tcagaggtcc agaacagata gcggtgcgtt gcttcgttca cctctttcag cgcatcctcc
    10621 agttgcccgt tggagacttt cagcttggtc agttccagta gtagcgtgtc gccaccctgc
    10681 aacaatgagt tcagcaactc acgctgagtg cgcagttgtg cttccagaat acggttttgc
    10741 tcggcggtca gcggctgacc gtcggcctga tggatttgcc gtagcagcgg ctgtttatta
    10801 agcagatcct cataacgtaa ccgttgcaca cgcaactgcg ccatttcggt atcaagctgt
    10861 tgtggttttg gcatttctgg tagccgtgcc acctgcgccc gcagcgcttc accgagcagg
    10921 ttggacgatc ccagccattg cgactgttca cgcagcgtat tcaacgcctg ccggacctgt
    10981 aacgtctggc tggcagcctg acgctgttgc gaggcaacga gatccatccg ctgcgcctgt
    11041 tgattcagtg ccgccgatag ttcgcggtta attttgaatt gcgcgacgat atctttcggc
    11101 aaatcggcac tgttttctgc cagtaattcg gtactttcca gcgcccgctc cgcctcaagc
    11161 tgacgttggc tattcaactg attacgtaag gcctgcaaat acgcatccag ttgctggctc
    11221 tctttttccg ccagctctga gcgcaagcgc gctaattcct gacggttatt ggcagacagc
    11281 tgcgccagtt ccagttcatc aacgagcgct ttcaggcgtg cggagtcaga ctgcaacgcg
    11341 aaattttgtg cctgattgag cggagtattg ccggtaagcg ttcccaggcg gcgctcgatc
    11401 tcatttaact gacggcgggc atcggtttgc tgttgcggca gttgattcag tgaatcggca
    11461 atctcgcggg cgcgctcctg ctcttgctgg gcctgacggc tcttatccag caactgactg
    11521 ctgacctgga gaatttcctg attcagcgca tcggtagaca ttcccggcga cacgctgcgc
    11581 ggctcgtcac gcatgttgtt taattgtgcg cgcagagtag cggagagttt cgggtaatta
    11641 tcgataactt gctgatattg tttgatgcgc tcaagggaac cttttcgttc ctcaagcgca
    11701 tttaaggcag actggagtgc ctctatgact tccggctgcg cgggtttcgc cgcttttgcc
    11761 tgctccagtt cctgggtgat ttgtttgcta tcgggggccg tcgcggcgta cgccccccaa
    11821 ctgaggcacc aggccatcag aaaagtgata atcaggcgca cgtcagcgtt tcctttgatg
    11881 gattagacct ggtctttttt gtcgtcaacc aatgggctgg cgtcgtgttc tgcttcgatc
    11941 tcttcagcag gaagcggggc aggttcagcg tctggcgtaa caaaggtttc ggtagatact
    12001 gccagcggct ggccaatttt cgtgacagac aggctttcca gttgctcaac cagattcact
    12061 ttacccggtg caaacaggtt gataacggtg gaaccgagtt taaagcgccc catttcctgg
    12121 cctttaagca gtgccacaga accgtcgttt tccccggcag gccaggtcca gcgcttgatg
    12181 ataccttcgc gcggcggcgt aatggtgccc gcccagaccg tctcaatgct gccaacaatc
    12241 gtcgctccga ccagaatctg cgccattggg ccaaattcgg tatcgaaaag gcaaatcacg
    12301 cgttcgttac gggcaaacag attcggcacg ttctgagcgg tgagatggtt aacggagaag
    12361 agatcgcccg gcacgtagat catctcacgc agaataccgt tgcacggcat gtgtacgcgg
    12421 tggtagtcac gcggggagag gtaagtggtc acaaacgtac cgttgcggaa caggttcgcc
    12481 atcagatagt tgcctgccag caaggcttcg aggctgtagt tgtggccttt ggcttgcagg
    12541 attttatctt cttcgatttt acccaactgg ctgataacgc catcggcagg catgaccagg
    12601 acattcggat cggtatcgat tgggcgtact tcgtcacgca gcggacggac aaagaattcg
    12661 ttaaaggtgc ggtagctggc ggtgtccggc ttttgcgcct ctttcatgtc gaccttgtag
    12721 tatttaacga acagatcgat aaccagtttt gtcagccatc ctgcccgctt gcttgcgccc
    12781 caacccgcca ggcgagtaag ccatagtttc ggcagaatgt actgtagcga aagtttaaat
    12841 gaatttaaca aggtagcctc caggccattg ttttgtcgtt cctgatccgg ccaacatgcc
    12901 ggatcctgaa aaaaagggga cgattctaac gacggttagc ttaattgtca gtcatccgta
    12961 tcagaaaagt ttttacgcgt ttttacctgc gccatgcttt ccagaatacg gtgatagttt
    13021 tcgaaacggg tttccgcgat tttcccttcc tcaaccgctt cccggatagc gcagcccgga
    13081 tcggtatcgt gtttgcaatc gcgatattta cacagaccta aatagtcatg gaattcgaca
    13141 aagccctgag tgatttgttc cggctccagg tgccagaggc cgaactcacg cacaccaggg
    13201 gaatcaatca catcaccgcc gtgcgggaag tgatacagcc gagcggcggt cgtggtgtgc
    13261 tgaccgagcc ccgagttgtc agagacatcg ttggtcagga tctctttttg cagccccagc
    13321 aacgcattca gcaggctgga tttgccgacg ccagactgcc cggcaaaaat gctgatgcgc
    13381 ccggtcaacg cctcttccag cggttttagc ccatcctgag tatggctgga aaccatcaat
    13441 acgcgataac caatgttgcg gtagatatcc atctgctcgt tgacgaacgc catgccttcg
    13501 tcgtccagca ggtctatctt attgagcaca ataatcggct caatctgcaa ggtttcgcag
    13561 gccaccaggt aacggtcgat aatattgagc gacagctccg gcaaaatggc ggagacaatg
    13621 acaatctggt cgatgttggc ggcaataggt ttcacgccgt cgtagaaatc cggacgcgtc
    13681 aacactgagg tgcgctcatg caccgcttcc acgatccctt tgacatttac gccttccgcc
    13741 gccggtttac ccggacgcca gactacgcgg tcgccggtta ccagcgaacg gatggtacgg
    13801 cgaatattgc agcggtgaac gtcgccatcg gcggattcca catcagcgtg cataccaaag
    13861 cggctgatga cgataccttc atcaggctcg ccaaacagat tgtcgtcgta gtcgggcttc
    13921 tccttagacg ttttaagacg acgctggtga ttggcgttca cgcggcgctg ctggcctttg
    13981 gagagtttat ttttactcaa tcgtacagac tcctggtcgc ccctgatggg caaaacatct
    14041 atgatacacg caattgtgga tcaatatagt cactgtgaat gggtggaaaa tagcatgagt
    14101 gccaatgaaa acaacctgat ttggatcgat cttgagatga ccggtctgga tcccgagcgc
    14161 gatcgcatta ttgagattgc cacgctggtg accgatgcca acctgaatat tctggcagaa
    14221 gggccgacca ttgcagtaca ccagtctgat gaacagctgg cgctgatgga tgactggaac
    14281 gtgcgcaccc ataccgccag cgggctggta gagcgcgtga aagcgagcac gatgggcgat
    14341 cgggaagctg aactggcaac gctcgaattt ttaaaacagt gggtgcctgc gggaaaatcg
    14401 ccgatttgcg gtaacagcat cggtcaggac cgtcgtttcc tgtttaaata catgccggag
    14461 ctggaagcct acttccacta ccgttatctc gatgtcagca ccctgaaaga gctggcgcgc
    14521 cgctggaagc cggaaattct ggatggtttt accaagcagg ggacgcatca ggcgatggat
    14581 gatatccgtg aatcggtggc ggagctggct tactaccgcg agcattttat caagctgtaa
    14641 aatttttcct ggtcacgtaa gcgccggaat ccacttgcgg cggcgtgatt ttgacgctaa
    14701 attgaacgtc ttgctgataa tttcagcact tgagataaaa acgcaaaaaa aacttttttg
    14761 gggggttgca gagggaaaga tttctcgtat aatgcgcctc ccgtaacgac gcagaaatgc
    14821 gaaaattacg aaagcaaaat taagtagtac gcgggaatag ctcagttggt agagcacgac
    14881 cttgccaagg tcggggtcgc gagttcgagt ctcgtttccc gctccaaaat ttgaaaagtg
    14941 ctgcaaagca cagaccaccc aagcgggaat agc
//
LOCUS       DAZGAN020000116        14812 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGAN020000116 DAZGAN020000000
VERSION     DAZGAN020000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.116"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            63..1334
                     /locus_tag="ACHKYO_RS19805"
                     /old_locus_tag="pgaptmp_003961"
     CDS             63..1334
                     /locus_tag="ACHKYO_RS19805"
                     /old_locus_tag="pgaptmp_003961"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU4430723.1"
                     /translation="MTPAASVAQGIQVIYQDLSLFPNLSVWENIAVNHYHHGLFVNRR
                     RLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDARLIVMDEPTASLTH
                     QEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGELVGTFPAAEMTTKQ
                     LGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRVEAGEVVSIVGLLG
                     AGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVSEDRMSRGLVMAQS
                     IEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHLPVNTLSGGNAQRV
                     SIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVLMICDEIEEAWYQS
                     HRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            1327..2298
                     /locus_tag="ACHKYO_RS19810"
                     /old_locus_tag="pgaptmp_003962"
     CDS             1327..2298
                     /locus_tag="ACHKYO_RS19810"
                     /old_locus_tag="pgaptmp_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4430724.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            2295..3251
                     /locus_tag="ACHKYO_RS19815"
                     /old_locus_tag="pgaptmp_003963"
     CDS             2295..3251
                     /locus_tag="ACHKYO_RS19815"
                     /old_locus_tag="pgaptmp_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4430725.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            3338..4387
                     /gene="yahK"
                     /locus_tag="ACHKYO_RS19820"
                     /old_locus_tag="pgaptmp_003964"
     CDS             3338..4387
                     /gene="yahK"
                     /locus_tag="ACHKYO_RS19820"
                     /old_locus_tag="pgaptmp_003964"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HGU4430726.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRRELGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            4630..5445
                     /gene="yahL"
                     /locus_tag="ACHKYO_RS19825"
                     /old_locus_tag="pgaptmp_003965"
     CDS             4630..5445
                     /gene="yahL"
                     /locus_tag="ACHKYO_RS19825"
                     /old_locus_tag="pgaptmp_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HGU4430727.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWLDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            5858..6103
                     /gene="yahM"
                     /locus_tag="ACHKYO_RS19830"
                     /old_locus_tag="pgaptmp_003966"
     CDS             5858..6103
                     /gene="yahM"
                     /locus_tag="ACHKYO_RS19830"
                     /old_locus_tag="pgaptmp_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HGU4430728.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRGRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(6120..6791)
                     /gene="yahN"
                     /locus_tag="ACHKYO_RS19835"
                     /old_locus_tag="pgaptmp_003967"
     CDS             complement(6120..6791)
                     /gene="yahN"
                     /locus_tag="ACHKYO_RS19835"
                     /old_locus_tag="pgaptmp_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HGU4430729.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            6938..7213
                     /gene="yahO"
                     /locus_tag="ACHKYO_RS19840"
                     /old_locus_tag="pgaptmp_003968"
     CDS             6938..7213
                     /gene="yahO"
                     /locus_tag="ACHKYO_RS19840"
                     /old_locus_tag="pgaptmp_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HGU4430730.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(7314..8900)
                     /gene="prpR"
                     /locus_tag="ACHKYO_RS19845"
                     /old_locus_tag="pgaptmp_003969"
     CDS             complement(7314..8900)
                     /gene="prpR"
                     /locus_tag="ACHKYO_RS19845"
                     /old_locus_tag="pgaptmp_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HGU4430731.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAVIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAVTVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFIQLLLPELARESAKIPAPRLLTPQQALEKFKGDK
                     TAAANYLGISRTTFWRRLKN"
     gene            9139..10029
                     /gene="prpB"
                     /locus_tag="ACHKYO_RS19850"
                     /old_locus_tag="pgaptmp_003970"
     CDS             9139..10029
                     /gene="prpB"
                     /locus_tag="ACHKYO_RS19850"
                     /old_locus_tag="pgaptmp_003970"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HGU4430732.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            10074..11243
                     /gene="prpC"
                     /locus_tag="ACHKYO_RS19855"
                     /old_locus_tag="pgaptmp_003971"
     CDS             10074..11243
                     /gene="prpC"
                     /locus_tag="ACHKYO_RS19855"
                     /old_locus_tag="pgaptmp_003971"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HGU4430733.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            11277..12728
                     /gene="prpD"
                     /locus_tag="ACHKYO_RS19860"
                     /old_locus_tag="pgaptmp_003972"
     CDS             11277..12728
                     /gene="prpD"
                     /locus_tag="ACHKYO_RS19860"
                     /old_locus_tag="pgaptmp_003972"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HGU4430734.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPTFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            12768..14654
                     /gene="prpE"
                     /locus_tag="ACHKYO_RS19865"
                     /old_locus_tag="pgaptmp_003973"
     CDS             12768..14654
                     /gene="prpE"
                     /locus_tag="ACHKYO_RS19865"
                     /old_locus_tag="pgaptmp_003973"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HGU4430735.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
BASE COUNT         3527 a         3657 c         4029 g         3599 t
ORIGIN      
        1 ccggtgttta tcgcccggat gagggagcgg aaattacgct tggtgggaaa aactggtcaa
       61 agctgacacc cgccgcttcg gtggcgcagg ggattcaggt gatttatcag gacctctctt
      121 tatttcctaa cctgagtgtc tgggagaata tcgccgtgaa tcactatcac cacggtctgt
      181 ttgttaaccg ccgtcgtctg cgtgaggtgg cgcaggcggc aatgaccagt attaacgtca
      241 cattgccact ggatacactc gtttctgagc tttcaattgc ccgctgtcag ctggtagcaa
      301 tttgccgtgc actggcgcag gacgcacggt tgatcgtgat ggatgaacca accgcttcgc
      361 tgacccatca ggaagtgcaa gggttattac aggtggtgca tcaattgcgg gaacgcggga
      421 tctgcgtggt cttcgtcagt catcgtctgg aagaagtgat ggaagtttcc gaccgtattt
      481 cagtgctgaa agatggtgag ctggtcggga cttttccggc tgcagagatg accacaaaac
      541 aactcggttt cctgatgacc ggccaggagt tcgaatatca ggtgcgagag ttgtggcagg
      601 gaaaatccag caccccggtg cttgaggtgc gtaatttaag tcgtcatggg gaatatctaa
      661 atattaactt gcgggtggaa gcgggcgaag tggtatcgat tgttggcctg cttggtgcgg
      721 ggcgcacgga attatgtctg agtctgtttg gtatgacccg accggatgct ggcgagatcc
      781 tcatcaacgg ccagccggtt acactgcata gtaaccagga cgctatccgc cacggtattg
      841 gttatgtttc ggaagatcgc atgtcgcgcg gtttggtgat ggcgcaatct attgaagaca
      901 acatcattag cactgttttt cacaaggtaa aagatcgctt cggctttctg agtgaaacta
      961 aagttcgtga cctggtggac aggctaatca aggcattgac catcaaagtc tctgatcctc
     1021 atcttccggt gaatacgctt tctggtggta acgcgcagcg agtatcgatc gccaaatggc
     1081 tggcgattgg gccacgactg ttgattctcg atagcccgac agtcggtgtt gatatcgcca
     1141 acaaagcagg aatatacggc attatcagcg atctggcggc tcatggcatt gcggtattga
     1201 tgatctgcga tgaaattgaa gaagcgtggt atcaaagcca ccggattttg gtaatgcaaa
     1261 aaggtcagat cacccatagc ttcctgcctg acagcagttc tcaggccagg attgcggagg
     1321 tggtaaatgg ctgaactgaa aaaacgccat gaattctggc tggcattact gattgtggtg
     1381 ctgtttgttg gccttgcctg gcgcagcgac gagtttctga cattcggtaa tttgtacgat
     1441 ctcgccaata actatgccat gttgactatt ctcgcctgtg gtttgtttgt ggtgttgatt
     1501 tccggtggaa ttgatatttc gtttccagca atgaccatca ttgcgcaata cggcatggtg
     1561 ctgttgctgc aaaaaattgg tggcaacttc gctgtcgcgt ttgcactggc gggctgcatc
     1621 ggcattttac ttggcttaat taacgcctta ctggttaatc gcctacgggt gccttctatc
     1681 atcatcacta tctcgacgct gaatattttc tatggcctgc tgttatggtt gagtaaaggt
     1741 gtgtggctgt acgactttcc gccgtggttt gagcaggggg ttatgttgtt caagtacacc
     1801 gatgctgatg gctatgacta tggccttggt ctgccgctga tcgccatgat tacggtggtg
     1861 ctgctaacag cgtttatcat gaatttcacc agtgtagggc gcaaaattta tgcccttggc
     1921 gggaaccgcg aatcagccag tcgcatcggc tttagcgtgc tgaaactgca acttttcgtc
     1981 tatggctata tgggattgat gtctggcgct gcgggtgtag tgcagtcgtg gacggtgatg
     2041 actgtcgccc ccgattctct tctgggttat gagctgacag tactggctgc ggtggtgctt
     2101 ggcggcacta gtttgctcgg cgggcgcggc acgttaaccg gtactttgct cggcgtggtg
     2161 ttgttggcag tgatgcaaaa cgggctaaat ttattgggag tctcgtctta ctggcaaaca
     2221 ttgatcaccg gcatcatcat cgttgccagc attagtgcca cggcgtggag tcagcatcag
     2281 aaccggagtc tgctatgaaa aaatcgtggc gtaataacgt tgagttttac ttgatcggcc
     2341 tgctggtgct aactgttgcc gcgttcagca ttactatgcc ggagattttc tggtcgataa
     2401 gtaatttcca gtccgtcgct tcgcaaatgc ccgtgctggg cattctggcg ttggctatgg
     2461 cggtgaccat gctttgcggt ggcattaact tgtcaattat agccactgct aacgcctgtt
     2521 cactggtgat ggcgtgggtg gcgactcagt atccaccggg tattgctacg gttgtcgcta
     2581 cgttactggc gggcgctggc gcggcggtga taatcggttt atgtaacggc gtattgattg
     2641 ctggtattcg agtgtcgcca attcttgcaa cgcttgggat gatgacgttg ctcaaaggga
     2701 taaatattct ggtgacaggt ggtagcgcca ttgccaatta tccctcatgg gtgctgtggt
     2761 tgaatcatgc gcaatggttc ggtattccgc taccgatgtg gttgtttgcc gcagtggcac
     2821 tggggctatg gatattgctg gagaaaacgc cgctcggtaa agccatttac ctgattgggt
     2881 ctaacgaacg agcaacactt tactccggca ttaacactcg tcgggtgctg atttgggttt
     2941 atgtcatttc cgccttgctt tgtgcggtag cggcattttt gatgatgtcg aaactcaact
     3001 cggctaaggc ctcttatggc gaatcgtact tgctggtgtc aatcctcgcg gcagtactcg
     3061 gtggggttaa tccggacggc ggcagcgggc ggataattgg tatggtgctg gcgctgtttc
     3121 ttctgcaaat tattgaaagc ggcttcaata ttctcggtat aagcccctac ctgacgatgg
     3181 ccttatgggg aacgttgctg ctctgtttca ttcaggttcg cggcatgttg gggctggatc
     3241 gggtggttta aatattggct atattcaatg gacgcgtttt gccgcgatga catatcaggc
     3301 gtcgccaaat acacatagct aatcaggagt aaacacaatg aagatcaaag ctgttggtgc
     3361 atattccgct aaacaaccac ttgaaccgat ggatatcaca cggcgtgaac tgggaccgaa
     3421 tgatgtcaaa atcgaaatcg cttactgtgg cgtctgccat tccgatctcc accaggtccg
     3481 ttccgagtgg gcggggacgg tttacccctg cgtaccgggt catgaaattg tggggcgtgt
     3541 ggtagccgtt ggtgatcagg tagaaaaaca tgcgccgggc gatctggtcg gtgtcggctg
     3601 cattgtcgac agttgtaaac attgcgaaga gtgtgaagac gggctggaaa actactgtga
     3661 tcacatgacc ggcacctata actcgccgac gccggacgaa ccgggccata ctctgggcgg
     3721 ctactcacaa cagatcgtcg ttcatgagcg atatgttctg cgtattcgtc acccgcaaga
     3781 gcagctggcg gcggtggctc ctttgttgtg tgcagggatc accacgtatt cgccgctacg
     3841 tcactggcag gccgggccgg gtaaaaaagt gggcgtggtc ggcatcggcg gtctgggaca
     3901 tatggggatc aagctggccc acgcgatggg ggcgcatgtg gtggcattta ccacttctga
     3961 ggcaaaacgc gaagcggcaa aagccctggg ggccgatgaa gttgttaact cacgcaatgc
     4021 cgatgagatg gcggctcatc tgaagagttt cgatttcatt ttgaatacag tagctgcgcc
     4081 acataatctc gacgatttta ccaccttgct gaagcgtgat ggcaccatga cgctggttgg
     4141 tgcgcctgcg acaccgcata aatcgccgga agttttcaac ctgatcatga aacgccgtgc
     4201 gatagccggt tctatgattg gcggcattcc agaaactcag gagatgctcg atttttgcgc
     4261 cgaacatggc atcgtggctg atatagagat gattcgggcc gatcaaatta atgaagccta
     4321 tgagcgaatg ctgcgtggtg atgtgaaata tcgttttgtt atcgataatc gcacactaac
     4381 agactgaaaa aattaataaa taccctgtgg tttaacatat taacttcgct ctccacttaa
     4441 ctttttagtt aaggagagcg aaataatatc aaagtagcag taaaacctat aacgtaaatt
     4501 caaattgtta aattaactcc ctccagtaca caatacttct cacgttagtt atgagcgatt
     4561 tctgatagtg cctggtttaa tgagagcttt attatctgcg acgtttattt ttatttaaga
     4621 gagatcgcga tgatatcatt aaaagctccg cacaataatt tgatgccata tacgcaacaa
     4681 agcatactta atacggttaa aaacaatcag ttaccagagg atatcaaaag ctccctggtt
     4741 tcctgtgtcg atatattcaa ggttttgatt aaacaatatt atgattatcc ttatgattgt
     4801 cgtgatgatt tagtcgatga cgacaaactc attcatctca tggctgccgt acgagactgt
     4861 gagtggttag acgataacgc actcaccata aatgtgcagt ttaatgattt ccctggattt
     4921 tatgactgga tggattaccc tgatcacccg gttaagtttg tttttcgcat acttgaaaat
     4981 caaaaaggga cagtttgggt atatgaccag gatgatgcgt ttcttgatat taaagcaaat
     5041 gtgcaggctg gacgctttac cgggcttaag aaattagtgc agtttatcga ttcagtgcga
     5101 acagattgca aatgtattct tcttgaacac catatgcctt tgctgagaat tttccctaaa
     5161 gggaaagagt gtatgcgtgt ggaaaaatgg cttcgcgaaa tgtcgtctat tccggagaca
     5221 gacgcgccca ttaagcaggc gcttgcgcat ggattattat tacacttaaa aaatatccac
     5281 cctgtttttc ctgaatcact ggtgatgtta ttgctctctg ttctggacgt aaaaacatac
     5341 agagatgacg cacgattaaa tgaatggata tcgaatagag tgcaggaatt aggtgacaga
     5401 tattatcctg taaataagca cgttaaaatc cgctacactc tttagttcag aaagggttcc
     5461 acctgcaagt tgttattcca ccagagagtg aatcgcaaag caggtaatca tttcatctgt
     5521 atatccgtat gccaaatggc attccaccgc ctttttcgcc gatattttgt tgtgatcggc
     5581 gacgcttcgc ttaaaaaagc accagcagtg gtttcgcagc tatgcggtgt ataaaaaatg
     5641 atctcatgca gatgttttgt gaatgtgtgg gttgacattc atatgaaaaa aatcataatt
     5701 ccatcatgtt tgtgtggggt cttttctgta ccttacgcat cgcactcaag cctgacagaa
     5761 aatatgctgt aaggctcata tcaaaaaccg ccattagctc atcaggaaga gcagacgaca
     5821 accaggattg ttgtatggta cggggttcga ggcctcgatg gcggtccaac ttttcaaaac
     5881 tcttctgaat cagatcccct tattatcatc cctgcaaagt ggaacattgc cattgtttgg
     5941 ctattcagga cggggcagac caatgaaaaa agcccacaca ggggagagtg ggctgaaatg
     6001 ggaagctaaa gactcaagta aacttatcgg aaataaggac cacgcattac gggggctatc
     6061 atcgccgatg gccgggattc ggcaaatcag gttgattacg tagagagtaa attatctgct
     6121 caccgctgcg tcaccccttc gtaaatcagg cgtagcgcga atacaccaat aattgcacca
     6181 ataacccgac tggcaacgcg ttgcatacgc ccataagcac gacgcacagc gggcaaagaa
     6241 aacgcctgac taagaaaaac tcgccagata attgatgcga gcacaatccc cgcccaggcc
     6301 attaaacgtg cccatgttgg tgtttcggca tttaatgtta ctgagaaaat actaatgaaa
     6361 aataaaacgg tttgcgggtt agagagatcg gtaattaatc cgcggcgaaa aaagacatac
     6421 cagggggcgc taatcggttg ttgtagtgtg ctcatttgcg gtgttgactg gcggcgcatg
     6481 ctgcaccacg caaaccataa gagataagcg ccgccgacga ttctgataag cgaaaaaatc
     6541 tcctcacact gcgtaattag tgttgcaaga ccaaacaaac ccaacccgga ataaaatgca
     6601 tcgcccagcg ccacccccag cccggtcagc acacctgcgc gtcgaccgga agccaggctg
     6661 gtttgtacta ccacaaagag attggctccc ggattaaaaa aagtaatcac gaacagtcct
     6721 acggtcaggt aaacggcgtg caaaggatcc atagtgattt catccataaa taagtgaagt
     6781 aacttcatca tatttctacc aaaaataatc gggtgcgaaa gagatcacaa agtgtcttat
     6841 ttccggttac tggcgtttat gccctgactg aactaattat taatcaaccc aataatgtgg
     6901 gtgggtgata gtgtgataac aactctggag ccgtaatatg aaaataatct ctaaaatgtt
     6961 agtcggtgcg ttagcgtttg ccgttaccaa tgtctatgcc gctgaattga tgaccaaagc
     7021 ggaatttgaa aaagttgaat cgcagtatga aaaaataggt gatatttcaa ccagtaatga
     7081 aatgtcgacc gcagatgcga aagaggattt gatcaaaaaa gcggatgaaa aaggggctga
     7141 tgtgttggta ctgacctctg gtcaaactga caataagatc cacggcacgg caaatattta
     7201 taagaagaag taattctgaa acctatgtaa acatccccga tgcgtaaatt tatcggtgat
     7261 catcatctat tgaaatttat gccggataaa gcgttcgcgc tgcattcggc agttcagttt
     7321 ttcagccgcc gccagaacgt cgtccggctt atgcctaaat aattcgccgc tgctgtttta
     7381 tcgcctttaa atttctccag tgcctgttgt ggtgtcagta agcgtggagc gggaattttc
     7441 gccgactcgc gcgccagctc cggcagtagc agttgtataa actgcggcgt taaatccggc
     7501 gtcggttcca cacttaaaaa cagcgccagt cgttccatca tattgcgcag ttcacgaata
     7561 ttgcctggcc agtcgtagtg caccagcacg gtttcgcttg cctgtaatcc ctggcgtaat
     7621 gcggcagaaa acggggcgga gagcgccgcc agagacactt tcaaaaagct ttccgccagc
     7681 ggaagaatat ccgccacccg ctcgcgcagt ggtggcaatt gcagacgcaa aatactcagc
     7741 cgataaaaca gatcacggcg aaactgccct tgccgcatat cttcttccag attgcagtga
     7801 gtggcgctaa tgacccgcac atccaccgga acaggctgat gcccgccgac gcgagtaacc
     7861 tctttttctt ccagcacccg taacaggcga gtctgcaaag gtagcggcat ttcgccaatc
     7921 tcatccagaa acagcgtacc gccgtgggca atttcgaaca gcccggcgcg acctccgcgt
     7981 cgcgagccgg taaacgcccc ttcctcatag ccaaacagct ccgcttccag cagcgattcg
     8041 gctatcgccc cgcagttgac ggcaacaaac ggatgcgact ttttgccctg tcgcgcatcg
     8101 tggcgggcaa aatattcccg atgaatcgcc tgggccgcca gctctttgcc cgtccccgtt
     8161 tccccttcaa tcaacaccgc cgcactggag cgggcataca gcaaaatagt ctgccgtact
     8221 tgttccatct gtggtgattg accgagcata tcgcccagca cgtaacgagt tctcagggcg
     8281 ttgcgggtgg catcgtgagt gttatggcgt aacgacatgc gcgtcatatc cagcgcatcg
     8341 ctgaacgcct ggcgcacggt gacggcggaa tagataaaaa ttccggtcat tccggcttct
     8401 tctgccagat cggtaatcag ccccgcaccg accaccgctt cggtgccgtt agctttgagc
     8461 tcgttaatct gcccgcgggc gtcttcttcg gtaatgtagc tacgttgatc gaggcgcaaa
     8521 ttaaaggttt tttgaaacgc caccagtgcc ggaatagttt cctgataagt gacaacgccg
     8581 atagaagagg tgagctttcc ggcttttgcc agtgcctgta acacatcgta gccgctcggt
     8641 ttaatcaaaa taactggcac tgacaggcgg cttttcaggt acgcgccgtt agagccagcg
     8701 gcgatgacgg cgtcgcagcg ttcgtttgcc agtttcttgc ggatataggt cactgctttt
     8761 tcaaagccaa gctggatagg ggtaatgttc gccaggtgat caaactcgag gctgatatcg
     8821 cgaaacagct cgaacaggcg cgttacagat accgtccaga taaccggttt gtcgtcatta
     8881 agccgtggtg gatgtgccat agcgcaccgc aaagttaaga aaccgaatat tgggtttagt
     8941 cttgtttcat aattgttgca atgaaacgcg gtgaaacatt gcctgaaacg ttaactgaaa
     9001 cgcatatttg cggattagtt catgacttta tttataacaa attgaaatta aacatttaat
     9061 tttattcagt taattgtggc acaccccttg ctttgtcttt atcaacgcaa gtaacaagtt
     9121 gataacaaag gatgggctat gtctctacac tctccaggta aagcgtttcg cgctgcactg
     9181 tctaaagaaa atccattgca gattgttggc accatcaacg ctaatcatgc gctgttggcg
     9241 cagcgtgccg gatatcaggc aatttatctc tccggcggtg gcgtggcggc aggttcgctg
     9301 gggctgcccg atctcggtat ttctaccctt gatgatgtgc tgaccgacat tcgccgtatc
     9361 accgacgttt gttcgctgcc gctgctggtg gatgcggata tcggttttgg ttcttcggcc
     9421 tttaacgtgg cgcgtacggt gaaatcgatg attaaagccg gtgcggcagg attgcatatt
     9481 gaagatcagg ttggtgcgaa acgctgcggt catcgcccga acaaagcgat cgtctcgaaa
     9541 gaggagatgg tggatcggat ccgcgcggcg gtggatgcga aaaccgatcc tgattttgtg
     9601 atcatggcgc gcaccgatgc gctggcggta gaggggctgg atgcggcgat cgagcgtgca
     9661 caggcctatg ttgaagcggg tgccgagatg ttgttcccgg aggcgattac cgaactcgcc
     9721 atgtaccgcc agtttgccga tgcggttcag gtgccaatcc tcgccaacat taccgaattt
     9781 ggtgccacgc cgctgtttac caccgacgaa ttacgcagcg cccatgtcgc aatggcgctg
     9841 tacccgcttt cagcgttccg cgccatgaac cgcgccgctg aacatgtcta caacgtcctg
     9901 cgccaggaag gcacgcagaa aagcgtcatc gacaccatgc agacccgcaa cgaactgtac
     9961 gaaagcatca actactacca gtacgaagag aagctcgacg acctgtttgc ccgtagccag
    10021 gtgaaataaa acacaacgaa aaatatctcg accctacaaa tgacgaggac aatatgagcg
    10081 acacaacgat cctgcaaaac agtacccatg tcattaaacc gaaaaaatcg gtggcacttt
    10141 ccggcgttcc ggcgggcaat acggcgctct gcaccgtggg taaaagtggc aatgacctgc
    10201 attaccgtgg ctacgatatt cttgatctgg cggaacattg cgaatttgaa gaagtggctc
    10261 atctgctgat ccacggcaaa ctgccgaccc gtgacgaact cgccgcttac aaaacgaaac
    10321 tgaaagccct gcgtggttta ccggctaacg tgcgtaccgt gctggaagcc ttaccggcgg
    10381 cgtcacaccc gatggatgtt atgcgcaccg gtgtttccgc gctcggctgc acgctgccag
    10441 aaaaagaggg gcataccgtc tctggcgcgc gggatattgc cgacaaactg ctggcgtcgc
    10501 ttagctcgat tctcctttat tggtatcact acagccacaa cggcgaacgc atccaaccgg
    10561 aaaccgatga cgactccatc ggcggtcact tcctgcatct gctgcacggc gaaaagccat
    10621 cgcaaagctg ggaaaaggcg atgcatatct cgctggtgct gtacgccgaa cacgagttta
    10681 acgcctccac ctttaccagt cgggtgattg cgggcaccgg ctctgatatg tattccgcga
    10741 ttattggcgc gattggcgca ctgcgcgggc caaaacacgg cggggcgaat gaagtgtcgc
    10801 tggagatcca gcaacgctac gaaacgccgg acgaagccga agcagatatc cgcaagcgcg
    10861 tggaaaacaa agaagtggtc attggttttg gtcatccggt ttacaccatc gctgacccgc
    10921 gccaccaggt gattaaacgt gtggcgaagc agctctcgca ggaaggcggc tcgctgaaga
    10981 tgtacaacat cgccgatcgc ctggaaacgg tgatgtggga gagcaaaaag atgttcccca
    11041 atctcgactg gttctctgct gtttcctaca acatgatggg cgttcccacc gagatgttca
    11101 caccactgtt tgttatcgcc cgcgtcaccg gctgggcggc gcacattatc gaacaacgtc
    11161 aggacaacaa aattatccgt ccttccgcca attatgttgg accggaagac cgccagtttg
    11221 tcgcgctgga taagcgccag taaacctcta cgaataacaa taaggaaacg tacccaatgt
    11281 cagctcaaat caacaacatc cgcccggaat ttgatcgtga aatcgttgat atcgtcgatt
    11341 acgtcatgaa ctacgaaatc agctccaaag tagcctacga caccgcacat tactgcctgc
    11401 tcgacacgct cggctgcggt cttgaagctc tcgaataccc agcctgtaaa aaactgctgg
    11461 ggccaattgt ccccggcacc gtcgtaccta acggcgtgcg cgtccccgga actcagttcc
    11521 agctcgaccc cgtccaggcg gcatttaaca tcggcgcgat gatccgttgg ctcgatttca
    11581 acgatacctg gctggcggcg gagtgggggc atccttccga caacctcggc ggcattctgg
    11641 caacggcaga ctggctttcg cgcaacgcgg tcgccagcgg caaagcgccg ttgaccatga
    11701 aacaggtgct gaccggaatg atcaaagccc atgaaattca gggctgcatc gcgctggaaa
    11761 actcctttaa ccgcgttggc ctcgaccacg ttctgttagt gaaagtggct tccaccgccg
    11821 tggtcgccga aatgcttggc ctgacccgcg aggaaattct caacgccgtt tcgctggcgt
    11881 gggtggacgg tcagtcgctg cgcacctatc gccatgcgcc gaacaccggc acgcgtaaat
    11941 cctgggcggc gggcgatgcc acttcccgcg cggtacgtct ggcactgatg gcgaaaacgg
    12001 gcgaaatggg ttacccgtca gccctgactg cgccggtgtg gggcttctac gacgtctcct
    12061 tcaaaggtga atcgttccgc ttccagcgcc cgtacggttc ttacgtcatg gaaaatgtgc
    12121 tgttcaaaat ctccttcccg gcggagttcc actcccagac ggcagttgaa gcggcgatga
    12181 cgctctatga acagatgcaa gcagcaggca aaacggcggc ggatatcgaa aaagtgacca
    12241 ttcgcaccca cgaagcctgt attcgcatca tcgacaaaaa agggccgctc aataacccgg
    12301 ctgaccgcga ccactgcatt caatacatgg tggcgatccc gctgttgttc gggcgcttaa
    12361 cggcggcaga ttacgaggac aacgttgcgc aagataaacg cattgacgcc ctgcgcgaga
    12421 agatcaattg ctttgaagat ccgacgttta ccgctgacta ccacgacccg gaaaaacgcg
    12481 ccatcgccaa tgccattact cttgagttca ccgacggcac gcgctttgaa gaagtggtgg
    12541 tggagtaccc cattggtcat gctcgccgcc gtcaggatgg cattccgaag ctggtggata
    12601 aattcaaaat caatctcgcg cgccagttcc cgactcgcca gcagcagcgc attctggagg
    12661 tttctctcga cagagctcgc ctggaacaga tgccggtcaa tgagtatctc gacctgtacg
    12721 tcatttaagt aaacggcggt aaggcgtaag ttcaacagga gagcattatg tcttttagcg
    12781 aattttatca gcgttcgatt aacgaaccgg agcagttctg ggccgagcag gcccggcgta
    12841 ttgactggca gacgcccttt acgcaaacgc tcgatcacag caatccgccg tttgcccgtt
    12901 ggttttgtga aggccgaacc aacttgtgtc acaacgctat cgatcgctgg ctggagaaac
    12961 agccagaggc gctggcgctg attgccgtct cttcggaaac agaagaagag cgcaccttta
    13021 cctttcgcca gcttcatgac gaagtaaacg cggtggcgtc aatgctgcgc tcgctgggcg
    13081 tgcagcgtgg cgatcgggtg ctggtgtata tgccgatgat tgccgaagcg catattaccc
    13141 tgctggcctg cgcgcgcatt ggcgctattc actcggtggt gtttggtgga tttgcctcgc
    13201 acagcgtggc ggcgcgaatt gatgatgcta aaccggtgct gattgtctcg gctgatgccg
    13261 gagcgcgcgg tggcaaaatc attccctata aaaaattgct cgacgatgcg ataagtcagg
    13321 cgcagcacca gccgcgccat gttttgctgg tggatcgcgg gctggcgaaa atggcgcgcg
    13381 tcagcgggcg ggatgtcgat ttcgcgtcgt tgcgccatca acacatcggc gcgcgggtac
    13441 cggtggcgtg gctggaatcc aacgaaacct cctgcattct ctacacctcc ggcacgaccg
    13501 gcaaacctaa aggcgtgcag cgtgacgtcg gcggatatgc ggtggcgctg gcgacctcga
    13561 tggacaccat ttttggcggc aaagcgggcg gtgtgttctt ttgtgcttcg gatatcggct
    13621 gggtggtggg gcattcgtat atcgtctacg cgccgctgct ggcggggatg gcgactatcg
    13681 tttacgaagg attaccgacc tggccggact gcggcgtgtg gtggaaaatc gtcgagaaat
    13741 atcaggttag ccggatgttc tcagcgccga ccgccattcg cgtgctgaaa aaattcccta
    13801 ccgctgaaat tcgcaaacac gatctctcgt cgctggaagt gctctatctg gctggagaac
    13861 cgctggacga gccgaccgcc agttgggtga gcaatacgct ggatgtgccg gtcatcgaca
    13921 actactggca gaccgaatcc ggctggccga ttatggcgat tgctcgcggt ctggacgaca
    13981 ggccgacgcg tctgggaagc cccggcgtgc cgatgtatgg ctataacgtg cagttgctta
    14041 atgaagtcac cggcgaaccg tgtggcgtca acgagaaagg gatgctggtg gtggaagggc
    14101 cgctgccgcc ggggtgtatt cagaccatct ggggcgatga cgaccgcttt gtgaagacgt
    14161 actggtcgct gttttcccgt ccggtgtacg ccacctttga ctggggcatc cgcgatgctg
    14221 acggttatca ctttattctc gggcgcactg acgatgtgat taacgttgcc gggcatcggc
    14281 tgggcacgcg cgagattgaa gagagtattt ccagtcatcc gggcgttgcc gaagtggcgg
    14341 tggttggggt gaaagatgcg ctgaaagggc aggtggcggt agcgtttgtc attccgaaag
    14401 agagcgacag tctggaagat cgtgatgtgg cgcattcgca agagaaggcg attatggcgc
    14461 tggtggacag ccagattggc aactttggcc gcccggcgca cgtctggttt gtctcgcaat
    14521 tgccaaaaac gcgatccgga aaaatgctgc gccgcacgat ccaggcgatt tgcgaaggac
    14581 gcgatcctgg agatctgacg accattgatg atccagcgtc gttggatcag atccgccagg
    14641 cgatggaaga gtaaggtatt gtcggatgcg tcgcgtggcg catccgacac catgcgccga
    14701 tgcctgatgc gacgcttacc gcgtcttatt atgcctacag gcctgaaccg taggtcggat
    14761 aaggcgctcg cgtcgcatcc gacaccccgc gccgatgcct gatgcgacgc tg
//
LOCUS       DAZGAN020000117        14729 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGAN020000117 DAZGAN020000000
VERSION     DAZGAN020000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.117"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..720
                     /gene="yneK"
                     /locus_tag="ACHKYO_RS19870"
                     /old_locus_tag="pgaptmp_003974"
     CDS             <1..720
                     /gene="yneK"
                     /locus_tag="ACHKYO_RS19870"
                     /old_locus_tag="pgaptmp_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HGU4430736.1"
                     /translation="KPVTMGRVFQTGVERVPFLFLNDFIEQFPMINPGAPIKRAHTPH
                     IEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKDPNLNTAI
                     LDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQESPHFQDLFLFFANMLL
                     HYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHEDLVKNRP
                     RSPVRPPIPATSKTPDLPERH"
     gene            870..2060
                     /gene="ydeA"
                     /locus_tag="ACHKYO_RS19875"
                     /old_locus_tag="pgaptmp_003975"
     CDS             870..2060
                     /gene="ydeA"
                     /locus_tag="ACHKYO_RS19875"
                     /old_locus_tag="pgaptmp_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HGU4430737.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2085..2750)
                     /gene="marC"
                     /locus_tag="ACHKYO_RS19880"
                     /old_locus_tag="pgaptmp_003976"
     CDS             complement(2085..2750)
                     /gene="marC"
                     /locus_tag="ACHKYO_RS19880"
                     /old_locus_tag="pgaptmp_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HGU4430738.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2962..3396
                     /gene="marR"
                     /locus_tag="ACHKYO_RS19885"
                     /old_locus_tag="pgaptmp_003977"
     CDS             2962..3396
                     /gene="marR"
                     /locus_tag="ACHKYO_RS19885"
                     /old_locus_tag="pgaptmp_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HGU4430739.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3416..3799
                     /gene="marA"
                     /locus_tag="ACHKYO_RS19890"
                     /old_locus_tag="pgaptmp_003978"
     CDS             3416..3799
                     /gene="marA"
                     /locus_tag="ACHKYO_RS19890"
                     /old_locus_tag="pgaptmp_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HGU4430740.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3831..4049
                     /gene="marB"
                     /locus_tag="ACHKYO_RS19895"
                     /old_locus_tag="pgaptmp_003979"
     CDS             3831..4049
                     /gene="marB"
                     /locus_tag="ACHKYO_RS19895"
                     /old_locus_tag="pgaptmp_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HGU4430741.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4106..5545)
                     /locus_tag="ACHKYO_RS19900"
                     /old_locus_tag="pgaptmp_003980"
     CDS             complement(4106..5545)
                     /locus_tag="ACHKYO_RS19900"
                     /old_locus_tag="pgaptmp_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGU4430742.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(5570..7243)
                     /locus_tag="ACHKYO_RS19905"
                     /old_locus_tag="pgaptmp_003981"
     CDS             complement(5570..7243)
                     /locus_tag="ACHKYO_RS19905"
                     /old_locus_tag="pgaptmp_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HGU4430743.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(7299..7610)
                     /locus_tag="ACHKYO_RS19910"
                     /old_locus_tag="pgaptmp_003982"
     CDS             complement(7299..7610)
                     /locus_tag="ACHKYO_RS19910"
                     /old_locus_tag="pgaptmp_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGU4430744.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(7638..8960)
                     /locus_tag="ACHKYO_RS19915"
                     /old_locus_tag="pgaptmp_003983"
     CDS             complement(7638..8960)
                     /locus_tag="ACHKYO_RS19915"
                     /old_locus_tag="pgaptmp_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGU4430745.1"
                     /translation="MGLMASFERGMERFLVPVAVKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGVFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(9075..9386)
                     /locus_tag="ACHKYO_RS19920"
                     /old_locus_tag="pgaptmp_003984"
     CDS             complement(9075..9386)
                     /locus_tag="ACHKYO_RS19920"
                     /old_locus_tag="pgaptmp_003984"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4430746.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            9585..10283
                     /locus_tag="ACHKYO_RS19925"
                     /old_locus_tag="pgaptmp_003985"
     CDS             9585..10283
                     /locus_tag="ACHKYO_RS19925"
                     /old_locus_tag="pgaptmp_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4430747.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(10328..11227)
                     /gene="eamA"
                     /locus_tag="ACHKYO_RS19930"
                     /old_locus_tag="pgaptmp_003986"
     CDS             complement(10328..11227)
                     /gene="eamA"
                     /locus_tag="ACHKYO_RS19930"
                     /old_locus_tag="pgaptmp_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HGU4430748.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVVRPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            11422..12609
                     /gene="ydeE"
                     /locus_tag="ACHKYO_RS19935"
                     /old_locus_tag="pgaptmp_003987"
     CDS             11422..12609
                     /gene="ydeE"
                     /locus_tag="ACHKYO_RS19935"
                     /old_locus_tag="pgaptmp_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HGU4430749.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPVNIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            12736..12831
                     /gene="mgtS"
                     /locus_tag="ACHKYO_RS19940"
                     /old_locus_tag="pgaptmp_003988"
     CDS             12736..12831
                     /gene="mgtS"
                     /locus_tag="ACHKYO_RS19940"
                     /old_locus_tag="pgaptmp_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HGU4430750.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            12831..12935
                     /gene="mgtT"
                     /locus_tag="ACHKYO_RS19945"
                     /old_locus_tag="pgaptmp_003989"
     CDS             12831..12935
                     /gene="mgtT"
                     /locus_tag="ACHKYO_RS19945"
                     /old_locus_tag="pgaptmp_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HGU4430751.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(13050..13940)
                     /gene="dgcZ"
                     /locus_tag="ACHKYO_RS19950"
                     /old_locus_tag="pgaptmp_003990"
     CDS             complement(13050..13940)
                     /gene="dgcZ"
                     /locus_tag="ACHKYO_RS19950"
                     /old_locus_tag="pgaptmp_003990"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HGU4430752.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPMDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(14195..14587)
                     /gene="ydeI"
                     /locus_tag="ACHKYO_RS19955"
                     /old_locus_tag="pgaptmp_003991"
     CDS             complement(14195..14587)
                     /gene="ydeI"
                     /locus_tag="ACHKYO_RS19955"
                     /old_locus_tag="pgaptmp_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HGU4430753.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFANTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         4037 a         3504 c         3111 g         4077 t
ORIGIN      
        1 aagcctgtca ctatggggcg cgtatttcag accggcgtcg agcgtgtgcc atttctgttt
       61 ctgaatgatt ttattgaaca atttccgatg atcaaccctg gcgctcccat caaaagagcg
      121 catacaccac atattgaacc cctgcctccg gatcaccata ccgctgcaga ttacttacgc
      181 cagtttgatt tgcttgtcct gaattttatc tctcgcggta attttgtcat actcccccga
      241 ttatggaata actctgaggt tcacagatgg ttcgtcaata aagatcctaa cttgaacacc
      301 gccattctcg acataacgga cagcgaatta aaagaggatt tattgcaaag cctgatggat
      361 tcattaggtt ctaacaaaca tgtactaccc gaagtctgca tctgcttttt atccctttta
      421 gcagaacaag aatctcctca ttttcaggac ttgtttttat ttttcgccaa tatgttattg
      481 cactatcacc aatttatgaa tcccaatgaa agtgatttga atgacgtgtt gatgccagca
      541 tcattaagtg atgataaaat tatcaaacat atggcgcgca ggaccctcaa actttttgta
      601 aaaaatgaaa cgccgccaaa agttactcac gaagacctgg tgaaaaacag gcctcgctcc
      661 cctgtcagac cacctatacc cgcaacctcc aaaacgccag acctccctga acgtcattaa
      721 accgtgatgt taccgactct ctgacgcgtg aaagaatcag cgtcagagaa acggaaaacg
      781 cgatccagat cacaaatgca ttgtattcac atcattaacc gttttaagat catttcatca
      841 ctttttcgca actcacccga taatctgtta tgacaacaaa cactgtttcc cgcaaagtgg
      901 cgtggctacg ggtcgttacg ctggcagtcg ccgccttcat cttcaacacc accgaatttg
      961 tccctgttgg cctgctctct gacattgcgc aaagttttca catgcaaacc gctcaggtcg
     1021 gcatcatgtt gaccatttac gcatgggtag tagcgctaat gtcattgcct tttatgttaa
     1081 tgaccagtca ggttgaacgg cgcaaattac tgatctgcct gtttgtggtg tttattgcca
     1141 gccacgtact gtcgtttttg tcgtggagct ttaccgttct ggtgatcagt cgcattggtg
     1201 tggcttttgc acatgcgatt ttctggtcga ttacggcgtc tctggcgatc cgtatggctc
     1261 cggccgggaa gcgagcacag gcattgagtt taattgccac cggtacagca ctggcgatgg
     1321 tcttaggttt acctctcggg cgcattgtgg gccagtattt cggttggcga atgaccttct
     1381 tcgcgattgg tattggggcg cttatcaccc ttttgtgcct gattaagtta cttcccttac
     1441 tgcccagtga gcattccggt tcactgaaaa gcctcccgct attgttccgc cgcccggcat
     1501 tgatgagcat ttatttgtta actgtggtgg ttgtcaccgc ccattacacg gcatacagct
     1561 atatcgagcc ttttgtacaa aacattgcgg gattcagcgc caactttgcc acggcattac
     1621 tgttattact cggtggtgcg ggcattattg gcagcgtgat tttcggtaaa ctgggtaatc
     1681 agtatgcgtc tgcgttggtg agtacggcga ttgcgctgtt gctggtgtgc ctggcattgc
     1741 tgttacctgc ggcgaacagt gaaatacacc tcggggtgct gagtattttc tgggggatcg
     1801 cgatgatgat catcgggctt ggtatgcagg ttaaagtgct ggcgctggca ccagatgcta
     1861 ccgacgtcgc gatggcgcta ttctccggga tatttaatat tggaatcggg gcgggtgcgt
     1921 tggtaggtaa tcaggtgagt ttgcactggt caatgtcgat gattggttat gtgggcgcgg
     1981 tgcctgcttt tgccgcgtta atttggtcaa tcattatatt tcgccgctgg ccagtgacac
     2041 tcgaagaaca gacgcaatag ttgaaaggcc cattcgggcc ttttttaatg gtacgtttta
     2101 atgatttcca ggatgccgtt aataataaac tgcacaccca tacataccag caggaatccc
     2161 atcagacggg agatcgcttc aatgccaccc ttgcccacca gccgcataat tgcgccggag
     2221 ctgcgtaggc ttccccacaa aataaccgcc accaggaaaa agatcagcgg cggcgcaacc
     2281 atcagtaccc aatcagcgaa ggttgaactc tgacgcactg tggacgccga gctaataatc
     2341 atcgctatgg ttcccggacc agcggtactt ggcattgcta gcggcacaaa ggcaatattg
     2401 gcactgggtt catcttccag ctcttccgac ttgcttttcg cctccggtga atcaatcgct
     2461 ttctgttgcg gaaagagcat ccgaaaaccg ataaacgcga cgattaagcc gcctgcaatt
     2521 cgcagaccgg gaatcgaaat gccaaatgta tccatcacca gttgcccggc gtaatacgcc
     2581 accatcatga tggcaaatac gtacaccgag gccatcaacg actgacgatt acgttcggca
     2641 ctgttcatgt tgcctgccag gccaagaaat aacgcgacag ttgttaatgg gttagctaac
     2701 ggcagtaaca ccaccagccc caggccaatt gctttaaaca aatctaacat tggtggttgt
     2761 tatcctgtgt atctgggtta tcagcgaaaa gtataagggg taaacaagga taaagtgtca
     2821 ctctttagct aaccttgcat cgcattgacc aaaacttgaa ccgatttagc aaaacgtggc
     2881 atcggtcaat tcatttattt gacttatact tgcctgggca atattatccc ctgcaactaa
     2941 ttacttgcca gggcaactaa tgtgaaaagt accagcgatc tgttcaatga aattattcca
     3001 ttgggtcgct taatccatat ggttaatcag aagaaagatc gtctgcttaa cgagtatctg
     3061 tctccgctgg atattaccgc ggcacagttt aaggtgctct gctctatccg ctgcgcggcg
     3121 tgtattactc cggttgaact gaaaaaagtg ttgtctgtcg accttggagc actgacccgt
     3181 atgctggatc gcctggtctg taaaggctgg gttgaaaggt tgccgaaccc gaatgataag
     3241 cgcggcgtac tggtaaaact taccaccagc ggcgcggcaa tatgcgaaca atgccatcaa
     3301 ttagttggcc aggacctgca tcaagaatta acaaaaaacc tgacggcgga cgaagtggca
     3361 acacttgagc atttgcttaa gaaagtcctg ccgtaaacaa aaaagaggta tgacgatgtc
     3421 cagacgcaat actgacgcta ttaccattca tagcattttg gactggatcg aggacaacct
     3481 ggaatcgcca ctgtcactgg agaaagtgtc agagcgttca ggttactcta aatggcacct
     3541 gcaacggatg tttaaaaaag aaaccggtca ttcattaggc caatacatcc gcagccgtaa
     3601 gatgacggaa atcgcgcaaa agctgaagga aagtaacgag ccgatactct atctggcaga
     3661 acgatatggc ttcgagtcgc aacaaactct gacccgaacc ttcaaaaatt actttgatgt
     3721 tccgccgcat aaataccgga tgaccaatat gcagggcgaa tcgcgctttt tacatccatt
     3781 aaatcattac aacagctagt tgataacgtg acaacgtcac tgaggcaatc atgaaaccac
     3841 tttcatccgc aatagcagct gcgcttattc tcttttccgc gcagggcgtt gcggaacaaa
     3901 ccacgcagcc agttgttact tcttgtgcca atgtcgtggt tgttccccca tcgcaggaac
     3961 aaccaccgtt tgatttaaat cacatgggta ctggcagtga taagtcggat gcgctcggcg
     4021 tgccctatta taatcaacac gctatgtagt tattgtcagg ctctgatcac agagcctgac
     4081 atctccgtaa tatcttcctc aagtctcaaa tattttcgcc attgttggcg atgagatccc
     4141 gataccagta aaacgacgct ttacgtatcc gttccagcgt tcccttccct tcgttgtctt
     4201 tatcgacata aatcattccg tagcgctttt tcatttcccc tgttccggca gaaaccaggt
     4261 caatgcagcc ccacggggtg tagccaatta agtcaacacc atcttcaact acggcttttt
     4321 tcatttcccg aatatgggaa gcaaagtaat caatgcgata gtgatcgttc accgtgccgt
     4381 cagcttgtct ctggtcaacc gcaccaaatc cattttcgac aataaacagc ggcaactgga
     4441 aatgatccca gaaccagttg agtgaataac gtagccctgc cggatcaatt tgccatcccc
     4501 attcggatgt atcgatataa gggttgctga ccaaatctcg tggttcaaca taatccagtt
     4561 gcgggttatc gggcgaaaat tgtgtcgtaa aagacatgta gtagctaaag ccgataaagt
     4621 cgacacaacc actggcaaga atcgcgttat cttctggtgt gatatcgagg ttgaatcctt
     4681 tcctggcaaa gtaattcagc atatgttgcg gataatatcc acgagcatga acatcagtaa
     4741 accagtaacg acgatgcatt gctttcgtgg ccatcatcat atcgttgggt gcacacgtca
     4801 gaggatagat gggacacatg gcgatcatac agccaatgtt aaattcagga ttgatcgatt
     4861 ttccagtctg taccgccagg gcactggcaa ctaactcgta atgtaccgcc tggtacatta
     4921 tttgttcgcg ctcattgata tcttcctctg gcgaatacaa gataccggaa ttagtaaatg
     4981 gacacaggct ttcgctgaaa ttcacctgat tattgatttc gttaaacgtc atccagtact
     5041 ttactttttc tttatagcgc gtgaagaccg ttgatgcgaa gcggataaaa aagtcgatca
     5101 gtttacggtt tcgccagcca ccatattttg tcaccagatg ataaggcatc tcaaaatgtg
     5161 aaagcgtcac cacaggttcc attccctgct taaggcattc atcgaacaga tcatcataaa
     5221 attgtaaacc ctcttcattc ggctcctgtt cgtcaccttg cggaaagatt cgtgtccagg
     5281 caatggaagt tcgaaagcat ttgaatccca tctcggcaaa taactgaata tctgttttat
     5341 agcgatgata aaaatcaatt gcttcatgat tgggataatt aagcccgtcg ataacgcctt
     5401 ctgtcacttc acgcggcacc ccgtgagcgc cagcagtcat cacatcagcg atgctgatgc
     5461 cttttcctcc ttcattccag ccaccttcca actgatgcgc ggctaccgca ccgccccata
     5521 aaaaaccttt tttaaatcct gacatacaca actccttaac taaaatagat taccaccaga
     5581 cttccgcctg gatgccggcc ataaactcgt ctttgctgtt ctcattaaac ttaaaattcg
     5641 ataattcgtt atccagaata ttgatatacg ttccatagaa gcgaatttct ggtcgtgaac
     5701 ctaaaatgct ttcacccact ttcaatgcat gccagagtgt cgttttataa gccgattcat
     5761 ttagtgttac accctgctga gttttgttct gttgcttaaa ccagcctaat tcaagccccg
     5821 tctgattcca tgtattccag atccaggccg gtcttattac agtgcgaata ctgtcaaaat
     5881 cactatgagc accactttca taactataaa catcttcgcc gtgagaatag acaagcgcgt
     5941 tagccataat aatattgtca gtaagataca tctcgccctg agagattaaa cgccaggcga
     6001 tcccattggt atgatcacca tagtaatagc gaccatgcgc catcgtatta ctggcatctg
     6061 agaaactggc aaaactgctg gcataggaat tattggcaac ctgtaacgta aattcattaa
     6121 acgtatcgcg ttgcaggttt tgccgtaaaa cagaagtcag catccagcta tctttcattt
     6181 caaaataact gttgtcattt tcgttatctt gttgttgatc cgttttatta ggtgcggaat
     6241 atttagccat taatgataat gtcgcatcat cccataacgg gatattgcga taacgaacat
     6301 ccacagaatt ggtattcatc tggctggtac gcgaaaaatc acgagagtaa acatcgacat
     6361 catcacggct taaggacata tcaaacagcc ctacaccaag tgcccagttt tcaatcccca
     6421 cacccgcggc gacatccgtg gttaaggttt tccagtccag catctggatc tcatattgcg
     6481 ggagtttatg tttgccgacc cagaagtctg cctctggtgc gaagggtaaa aaacctcgcg
     6541 ttgtcaggta aatatcgctg aactgcatga tattttcatt ggcactgtca ccaaaccagg
     6601 catcgttata ctgctcaccc acgttgccat cataggtaac gaccgcattt gccgttttac
     6661 cgtcctgatt ataaacacgc tgatttaagg tcaggtcaaa ccaaccgctc atctcattac
     6721 caaaacgtcc cagagaaccc gctgcataag tttgtggtga gccgtgatta ccagtacccc
     6781 agcctgagcg gaagtacccc tgatagctga acccaatatc atcctttata tatttactga
     6841 tatctttcag ggtcacgctt tcgatagtgg ttttgtcgcc tttactttca ataacggcaa
     6901 cttgctgcga tccatttata gtatgcgctg cggcagacga gttctgttca ccgttttcat
     6961 ttttgatgtt atcagcgacg ggagatgtcg ctgatatttc agcaagcgag gctgatttat
     7021 atttgttttc cgtgatgctt ttttgtaagg tcgaaagtcg ggatttatat actcccagtt
     7081 cattctgtgt tgctttcagc tcttgtttat tttgcgacaa ttcattttca agcaattcaa
     7141 ggcgctgctc tatggttaat tttgttgcca tcgcgttcaa tggaaacaat gctgtaataa
     7201 tagaaaaaga caaaaggctg acgttaagcg tctttatatt catgacttat ccttaagagt
     7261 ataataattt cctccagcta tcgcactgga ggaaaaaatc aggcatggag ttcttttctt
     7321 aatgaaataa tttccctggc taaatcctgg cataatattg cattcatcaa atgatcctgt
     7381 gcatgaatca gaattaaatt aacttttatt ttgccacagc cttcatcctg actaattaat
     7441 gcggtctgaa tcttatgtgc ctgaagacag gcagattcag aactggccat tagctgagtt
     7501 gcgccctgcc agtctttttg cccggcacaa cgtatggctt ccatcgcgtc agaacgtgcc
     7561 tgccccgcgt tgaccagtaa ctccataaca agttcttcat ctgcaaacat cacaatctcc
     7621 ttcctgggta aatggaatta tccgttagtt gtcatgttat tgactttgtt ggcggcaata
     7681 acaaaaggta aatagatcat tgtcgccaca cctaaacata aaaaaccaac cagtaaagcc
     7741 agccagttcc ccccggttcc gaggaaagca attaaaggtc cgggcgtagt ccagggcacg
     7801 gcataagcaa ccggtggaat aatttccatc gaaacaaaga agtaaccaat ggcacagtta
     7861 acaatgggaa ccataataaa cggcaccatc atgatggggt taagtacaat aggaaggcca
     7921 aacattatcg gttcattgat attgaaaata ccggggataa atgacatttt tgccagatca
     7981 cggtattccg cacgcttaga agcgataaaa atagccaaca ataaccccaa agtcatacct
     8041 gaaccgccgc agttggcgaa tgcatcatta atagaagtcc aggtaatcgg atatggcgcg
     8101 ccccaggtag tgccatgttg agcggcccag gagagattct ccagattagc ctcagaaaaa
     8161 acggtttctc gaattgccga tgtggtatta ggtccgtgga tccccagcac ccacaggaaa
     8221 ttacccacag cccccaggat aattaccgca aagatattcg ttcccatatg tttgagcggc
     8281 gtctggatta atgtgtaaat aagatcgttt agtcctgccg gggatatcag tgtcaggcaa
     8341 taattaagtg cggaaaagaa caccatgaca aaaaatattg gcaataaaac tttaaatgaa
     8401 cgcgctaccg caggcggtac agctgccggc atcgttatgg tgatttttgg gtttcgagct
     8461 aagcgacaaa atatttcact ggtgatcaat gcaacgataa cagcaacaaa caccccctgc
     8521 gcgccgagat atttggtcgt caggaatgct tgcccatcta tcatctgata tggcgtataa
     8581 acaataaaaa atgctcctat tgccgttaat ccgcataaaa gatcatcttg ctcatagcta
     8641 atcgccatat tcctggcgac caggaaagca atcataattg acatgatatt tacagaacca
     8701 ttcattaccg gagtaaaaat agcttgtgct ttttcaaggt tggggaaaat gctattcaga
     8761 tgcagtaatc cggcaataaa accgtctggc gataatatgg caaagttaat taatataatt
     8821 aatgaacttg ccatgataat tggaaacgta aaaacgaatc catctctcac cgctgcaaca
     8881 tgtttttgtg agtttaactt gacagcaact ggaacaagaa aacgttccat tccacgttcg
     8941 aatgaggcca ttaatcccat ttttttatac ctgttgtttt ggatatttat acatcccaat
     9001 aaagcatgta tgccatatcc attttatgat gtgtgaaaag ttagttcagg taatagttta
     9061 ataatattag attcttactc ttgcagtagt ttcatcgcaa attcaagaac tgcctgtccg
     9121 ttcatggtgc cgtagtcttt catctcgata acggctacag gcattttccc ctgtgcaacg
     9181 gcttcaatct ctggtttctg aaagcgaact tgcgggccaa gtaaaacaac gtcaacttcg
     9241 ttatttttaa ttttcccttt agcctctgca atcgccaatg cggaaatatt aacttcaagt
     9301 gaaatagcgt tagcatgatc aatcatacgt ttaaccagca tactggttga catgcccgca
     9361 gcgcaaacta acaatatttt tttcatattt tcccttactg gtatataaca gattacattg
     9421 tttaaatatc atatcccgca gtagctatac gtcctggtac attgatcaca gtaatacagt
     9481 gataaagttg ttcaacatca gggtgattat taaacgactc ggccatacga taaaccaatc
     9541 aggactcaac tccttgtttt caatggctct taaaaggtgc aaaagtgatt tttcaaaaga
     9601 ttgcccgctt acttaaatcc gaaatcaatg gcaattcatg gcatgttggt gatttgttgc
     9661 cgtcagaagc ggaactcgct gttcgttata atgtttcgcg taataccctg cgtaaggcat
     9721 tatccttgct ggaaggcgaa ggaattattc acagaaagca tggttcagga acatacattc
     9781 aaaaaaagaa ttttgttgca cacattgatc acatgaatag tttcagtgaa attgcgcata
     9841 aaagcggcaa agaggcaggg agtcagatta tgaaatttga agtgcaggat gcttcaccca
     9901 ctattgcgac tgagctgaat ttagtgactg gtgagcaggt ttattacata aagagactgc
     9961 gattcattga ggataatgca gcacaattag aagaaacgtg gatgtcagta gcacgttttc
    10021 ctgatttaac cgtgtcacat atgcaaaaat ccaaattttc atatattgag aatgaatgcg
    10081 ggatcaaaat cattggcacc tttgaaactt tctcccctac ttttcctacc ccagaaatcg
    10141 ccagtatttt acggatcagc ccacgagatc ccatacttaa aattcagacc caggctgtgg
    10201 acagtaactc tattccgctg gattattcgt tactttacag caatattttc gagttccagg
    10261 taaagtactt tttcccgcga taaaagcaga cgcacagagt gctgacatca cattctgtgc
    10321 gtatggttta gcctctaacc tttaccgctt tacgccaccg caagccaaat acattgatat
    10381 acagcccggt cataatgagc accgcaccga aaaattgtag acccgttaag cgttcatcca
    10441 acaatagtgc cgcacttgcc agtccgacta cgggcaccag taacgataac ggtgcaaccc
    10501 gccaggtttc atagcgtcca agtagtgttc cccagatccc ataaccaaca attgtcgcca
    10561 caaacgccag atacatcaga gacaaaatgg tggtcatatc gatagtaacc agactgtgaa
    10621 tcatggttgc ggaaccatcg agaatcagcg aggcaacaaa gaagggaatg attgggatta
    10681 atgcgctcca gattaccagc gacatcaccg ccggacgcgt tgagtgcgac atgatctttt
    10741 tattgaagat gttgccacac gcccaactaa atgctgccgc cagggtcaac ataaagccga
    10801 gcatcgccac atgctgaccg ttcagactat cttcgattaa caccagtacg ccaaaaatcg
    10861 ctaaggcgat ccccgccaat tgtttgccat gcaggcgctc cccgaaagta aacgcgccaa
    10921 gcacgatagt aaaaaacgcc tgtgcctgta acaccagcga agccagtcca gcaggcatac
    10981 caaaattaat ggcgcagaac agaaaggcaa actgcgcaaa actgatggtt aatccatacc
    11041 ccagcagcaa attcagtggt actttcggtc gtacgacaaa aaagatagcc ggaaaagcga
    11101 ccagcataaa gcgcaaaccg gccagcatca gcggtggcat gttatgaagc cccactttga
    11161 tgaccacaaa atttagcccc catacgacca ctaccagtag cgccaacacc ccatcttttc
    11221 gcgacattct accgcctctg aatttcatct tttgtaagca atcaacttag ctgaatttac
    11281 ttttctttaa cagttgattc gttagtcgcc ggttacgacg gcattaatgc gcaaataagt
    11341 cgctatactt cggatttttg ccatgctatt tctttacatc tctaaaacaa aacataacga
    11401 aacgcactgc cggacagaca aatgaactta tccctacgac gctctaccag cgcccttctt
    11461 gcctcgtcgt tgttattaac catcggacgc ggcgctacgc tgccatttat gaccatttac
    11521 ttgagtcgcc agtacagcct gagtgtcgat ctaatcggtt atgcgatgac aattgcgctc
    11581 actattggcg tcgtttttag cctcggtttt ggtatcctgg cggataagtt cgacaagaaa
    11641 cgctatatgt tactggcaat taccgccttc gccagcggtt ttattgccat tcctttagtg
    11701 aataacgtga cgctggtggt gctctttttt gcccttatta actgcgccta ttccgttttt
    11761 gctaccgtgt tgaaagcctg gtttgccgac aatctttcgt ccaccagcaa aacgaaaatc
    11821 ttctcaatca actacaccat gctaaacatt ggctggacca tcggtccgcc gctcggcacg
    11881 ctgttggtga tgcagagcat caatctgccc ttttggctgg cagctatctg ttccgcgttt
    11941 cccatgcttt tcattcaaat ttgggtaaag cgcagcgaga aaatcatcgc cacggaaaca
    12001 ggcagtgtct ggtcgccgaa agttttatta caagataaag cactgttgtg gtttacctgc
    12061 tctggttttc tggcttcctt tgttagcggc gcatttgctt catgcatttc acaatatgtg
    12121 atggtgattg ctgatgggga ttttgccgaa aaggtggtcg cggttgttct tccggttaat
    12181 gctgccatgg tagttacgtt gcaatattcc gtgggccgcc gccttaaccc ggttaacatc
    12241 cgcgcgctga tgacagcagg caccctctgt ttcgtcatcg gtctggttgg ttttattttt
    12301 tccggcaaca gcctgctatt gtggggtatg tcagctgcgg tatttactgt cggtgaaatc
    12361 atttatgcgc cgggcgagta tatgttgatt gaccatattg cgccgccagg aatgaaagcc
    12421 agctattttt ctgcccagtc tttaggctgg cttggtgctg caattaaccc attagtgagt
    12481 ggcgtagtgc taaccagcct gccgccttcc tcgctgttta tcatcttagc gttggtgatc
    12541 attgctgcgt gggtgctgat gttaaaaggg attcgtgcaa aaccgtgggg gcagcccgcg
    12601 ctttgttgat ttaagtcgaa cacaataaaa atttaattca gccttcgttt aggttacctc
    12661 tgctaatatc tttctcattg agatgaaaat taaggtaagc gaggaaacac accacaccat
    12721 aaacggaggc aaataatgct gggtaatatg aatgttttta tggccgtact gggaataatt
    12781 ttattttctg gttttctggc cgcgtatttc agccacaaat gggatgacta atgaacggag
    12841 ataatccctc acctaaccgg ccccttgtta cagttgtgta caaggggcct gatttttatg
    12901 acggcgaaaa aaaaccgcca gtaaaccggc ggtgaatgct tgcatggata gatttgtgtt
    12961 ttgcttttac gctaacaggc attttcctgc actgataacg gatcgttgac acagtagcat
    13021 cagttttctc aatgaatgtt aaacggagct taaactcggt taatcacatt ttgttcatca
    13081 ataaacatgc agcgatttct tccggtttgc ttaccctcat acattgcccg gtctgctctt
    13141 ccaatgacca catccatagg ctcttcagga aatgcgcgac tgacacctgc tgtcacggta
    13201 atgttgatat gcccttcaga atgcgtgatg gcatggttat cgactaactg gcaaattctg
    13261 acacctgcac gacatgcttc ttcatcatta gccgctttga caataatgat aaattcttcg
    13321 cccccgtagc gataaaccgt ttcgtaatca cgcgtccaac tggctaagta agttgccagg
    13381 gtgcgtaata ctacatcgcc gattaaatgc ccgtaggtat cattaaccaa tttaaatcgg
    13441 tcaatatcca acaacattaa ataaagattc agaggctcag cgttgcgtaa ctgatgatca
    13501 aaggattcat caagaacccg acgccccggc aatcccgtca aaacatccat attgctacgg
    13561 atcgtcagca aataaatttt gtaatcggtt aatgccgcag taaaagaaag caacccctcc
    13621 tgaaaggcgt cgaaatgcgc gtcctgccag tgattttcaa caatagccag cattaattcc
    13681 cgaccacagt tatgcatatg ttgatgggca gaatccatta gccgaacgta aggtaattca
    13741 tcgttatcga gtggccccag atgatcaatc caccgaccaa actggcacag tccataagaa
    13801 tggttatccg ttatttctgg cttactggca tctctcgcga ccacgctgtg aaacatactc
    13861 accagccact ggtagtgggc atcgatagcc ttattgagat ttaacaagat ggcatcaatt
    13921 tccgttgttt tcttgatcat tgccactcct ttttcacagt tccttgtgcg cgctattcta
    13981 acgagagaaa agcaaaatta cgtcaatatt ttcatagaaa tccgaagtta tgagtcatct
    14041 ctgagataac attgtgattt aaaacaaaat cagcggataa aaaagtgttt aattctgtaa
    14101 attacctctg cattatcgta aataaaagga tgacaaatag cataacccaa taccctaatg
    14161 gcccagtagt tcaggccatc aggctaattt atttttattt ctgcaaatga gtgacccgaa
    14221 cgacggccgg cgcgcttttc ttatccagac tgccgctaat gttgatcatc tggtccggct
    14281 gaacttctcg tccatcaaag acggccgcag gaataacgac attaatttca ccgctcttat
    14341 cgcgaaaaac gtaacggtcc tctcctttgt gagaaatcaa attaccgcgt agtgaaaccg
    14401 aagcgccatc gtgcatggta tttgcgaaat caacggtcat tttttttgca tcatcggttc
    14461 cgcgatagcc atcttctatt gcatgaggcg gcggtggcgc tgcatcctgt tttaaaccgc
    14521 cctggtcatc tgccaacgca taaggcatga caagaaaact tgctaataca atggcctgaa
    14581 atttcatact gactccttaa ttgcgtttgg tttgacttat taagtctggt tgctattttt
    14641 ataattgcca aataagaata ctgccaattg ttataaggca tttaaaatca ggcaactagc
    14701 tgtcaaatat tcagagaatt taactcact
//
LOCUS       DAZGAN020000118        14592 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGAN020000118 DAZGAN020000000
VERSION     DAZGAN020000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.118"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            176..1006
                     /gene="yjiC"
                     /locus_tag="ACHKYO_RS19960"
                     /old_locus_tag="pgaptmp_003992"
     CDS             176..1006
                     /gene="yjiC"
                     /locus_tag="ACHKYO_RS19960"
                     /old_locus_tag="pgaptmp_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU4430754.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKEPWPFNYVTETCRESDGITTTGRMLYRNLKI
                     TTVLDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1147..1920)
                     /gene="uxuR"
                     /locus_tag="ACHKYO_RS19965"
                     /old_locus_tag="pgaptmp_003993"
     CDS             complement(1147..1920)
                     /gene="uxuR"
                     /locus_tag="ACHKYO_RS19965"
                     /old_locus_tag="pgaptmp_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HGU4430755.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2135..3595)
                     /gene="uxuB"
                     /locus_tag="ACHKYO_RS19970"
                     /old_locus_tag="pgaptmp_003994"
     CDS             complement(2135..3595)
                     /gene="uxuB"
                     /locus_tag="ACHKYO_RS19970"
                     /old_locus_tag="pgaptmp_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HGU4430756.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3676..4860)
                     /gene="uxuA"
                     /locus_tag="ACHKYO_RS19975"
                     /old_locus_tag="pgaptmp_003995"
     CDS             complement(3676..4860)
                     /gene="uxuA"
                     /locus_tag="ACHKYO_RS19975"
                     /old_locus_tag="pgaptmp_003995"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HGU4430757.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5200..6543
                     /gene="gntP"
                     /locus_tag="ACHKYO_RS19980"
                     /old_locus_tag="pgaptmp_003996"
     CDS             5200..6543
                     /gene="gntP"
                     /locus_tag="ACHKYO_RS19980"
                     /old_locus_tag="pgaptmp_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HGU4430758.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6786..7688)
                     /gene="fimH"
                     /locus_tag="ACHKYO_RS19985"
                     /old_locus_tag="pgaptmp_003997"
     CDS             complement(6786..7688)
                     /gene="fimH"
                     /locus_tag="ACHKYO_RS19985"
                     /old_locus_tag="pgaptmp_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU4430759.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVVLYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7708..8151)
                     /locus_tag="ACHKYO_RS19990"
                     /old_locus_tag="pgaptmp_003998"
     CDS             complement(7708..8151)
                     /locus_tag="ACHKYO_RS19990"
                     /old_locus_tag="pgaptmp_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4430760.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8224..8754)
                     /gene="fimF"
                     /locus_tag="ACHKYO_RS19995"
                     /old_locus_tag="pgaptmp_003999"
     CDS             complement(8224..8754)
                     /gene="fimF"
                     /locus_tag="ACHKYO_RS19995"
                     /old_locus_tag="pgaptmp_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HGU4430761.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8764..11400)
                     /gene="fimD"
                     /locus_tag="ACHKYO_RS20000"
                     /old_locus_tag="pgaptmp_004000"
     CDS             complement(8764..11400)
                     /gene="fimD"
                     /locus_tag="ACHKYO_RS20000"
                     /old_locus_tag="pgaptmp_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HGU4430762.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQTPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSIIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNRSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFIVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNETVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11467..12192)
                     /gene="fimC"
                     /locus_tag="ACHKYO_RS20005"
                     /old_locus_tag="pgaptmp_004001"
     CDS             complement(11467..12192)
                     /gene="fimC"
                     /locus_tag="ACHKYO_RS20005"
                     /old_locus_tag="pgaptmp_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU4430763.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12229..12768)
                     /gene="fimI"
                     /locus_tag="ACHKYO_RS20010"
                     /old_locus_tag="pgaptmp_004002"
     CDS             complement(12229..12768)
                     /gene="fimI"
                     /locus_tag="ACHKYO_RS20010"
                     /old_locus_tag="pgaptmp_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4430764.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSEHVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12833..13381)
                     /gene="fimA"
                     /locus_tag="ACHKYO_RS20015"
                     /old_locus_tag="pgaptmp_004003"
     CDS             complement(12833..13381)
                     /gene="fimA"
                     /locus_tag="ACHKYO_RS20015"
                     /old_locus_tag="pgaptmp_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4430765.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
     gene            complement(13862..14458)
                     /gene="fimE"
                     /locus_tag="ACHKYO_RS20020"
                     /old_locus_tag="pgaptmp_004004"
     CDS             complement(13862..14458)
                     /gene="fimE"
                     /locus_tag="ACHKYO_RS20020"
                     /old_locus_tag="pgaptmp_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HGU4430766.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT         3614 a         3697 c         3467 g         3814 t
ORIGIN      
        1 ttttaaatga gcgttttttg atactttaga gtgtttccta ccctttaata tcaccatgta
       61 gagttgatat cactacaaca gaaacatgca tgtcatttgt ttacttcttt tttactacag
      121 aaaaatttat ctatttttct aaacatcagt gaaatactca taaaaggtaa ctattatgtc
      181 tatgccatta agcaatgccc ttcagagtca aattattaca gataatcact tccttcatca
      241 tccaaaagtc gacagtgagt taacacgtcg atatgaatat gcacgcctgg atacggaaaa
      301 tatctatctt ctgcctcttg ctcgtggcaa taaccataat tacgatggaa aaagcgttgt
      361 cgaaattaga aaacttgata ttagtaaaga gccctggcca tttaattatg tgacagaaac
      421 atgcagagaa tccgatggta ttacaacaac aggacgtatg ctctatcgaa acttgaaaat
      481 caccacagtt ttagacgaaa tatatggtgg tatatgtaaa aaggcgcatg ctgccacaga
      541 gttagcagaa ggattacggc taaatttatt tatgaagagc cctttcgatc ctgttgagga
      601 ctatacagtt catgaaataa cattaggccc tggttgcaat gttcctggtt atgccggaac
      661 cacgatagga tatatttcaa cccttccagc ttctcaggca aaaagatgga ccaatgagca
      721 gccaaggatt gatatctata ttgatcagat aatgactgtc tcaggggtgg caaattcttc
      781 cggattcgcc cttgccgcat tacttaatgc taatattgag ttgggtaatg acccaataat
      841 tggtatagaa gcctatcccg gtaccgcgga gatccatgca aaaatgggat ataaagttat
      901 cccgggtgat gaaaatgcac cactcaaacg tatgactctg caaccgtcat ctcttccaga
      961 actgtttgaa ttaaaaaatg gtgaatggaa ttatattggt aaataactac caggaacaat
     1021 gagtactaat attcaggtta tacagatatt taaacaatac cctgcaattt acaaagtaat
     1081 actcattgcg acttcttaat ttaagaaagc gcaaggaacg tttacccttg cgcttattat
     1141 aataagtcag gcgtcgactt tatccagcgg ccatgaatca aacagatagc catcaaaata
     1201 aatatcgtca acgttcgaga attccagcag acgttgctta acgttttcca gatgctgcca
     1261 cattgccagc ttcgccgctc gggcatcttt tttgattaac gcggcgagga tctgtttgtg
     1321 atcgcccaac cactctttgc gatacaggct gtcatccaga tggctgtgca actggatcca
     1381 cattggattg ttttcccgcc actgccagga ctgacggaac agctccacca gcatgctgtt
     1441 atgcgttgct tcggcaatag cgagatggaa ctgcatgtca ccgctttcgc tgctgcctgg
     1501 cgcactggaa gccagttcac gctcttccag ttgcaatgcc tgacgcattt tgacgatatc
     1561 ttcgcgggta gcctgcaaag cggcaaactc ggcgatgttg ctctccaata actgccgcgc
     1621 ctgtaacagc tcaaaaggac cggcatcgtt gcagacgttg gcatccggac tgtctgtgtt
     1681 ctggctgcct gagttatcaa gaacatagat accggcaccc cggcgtactt ccaccagccc
     1741 tttgatctcc agcatgatca gcgcttcacg taccaccgtc cgcgtgacat caagcatttc
     1801 tgcaatttca cgctccggcg gcagccgttc gccagggttg tacggcgtct ttatgatcag
     1861 atcgcggatc atcgccccga cttcctggta aggtctttgc gcagaggtgg cagatttcat
     1921 ggtgttgtgt ccgttaggtg cggttaatca aatttttgcc ggatgcggcg tgaacgcctt
     1981 atccggccta caacgggcaa ttttacggcc cccgttaatc tcaaactata ccattagcac
     2041 tggaagttgg cacgtacagg tgggcaaaaa aacgaaaact ggtcaaccaa atatcagttt
     2101 gattgaccag taagtctgta gggtaatcag ttagttacag cgcagccaca cactcgcgcg
     2161 caccgcgttc gcacagctgc tgatatgccg ccgtcactgc gccaacaaag tcggcattct
     2221 gcggcagatc atcggcaaaa ataccgctca ggcccagcag cgctttcacg cggtctgcgc
     2281 cctgatactg cgcgttaatc ttctggaact cggccagcat cgggtcgacc acgtcaatgg
     2341 cattaccctg ctcatccacg ccctgggtgt aacgcatcca gccagccacg cccagcgcca
     2401 ggtgacgcca gctaccgccg ttttgcaggt gcagacgcac cggatccagc agacgctgcg
     2461 gtaacttctg gctgccgtcc attgcaatct gccaggtacg gtgacgcaga gatgggttgc
     2521 tgaaacgctc gatcagcagc gtcgcatagg cgttcaggtc cgtaccttcc ggcatcgaca
     2581 gcgttggcgc ttgttcctgc atcatcaggg caaaggccgc tttgcgataa tccgggttag
     2641 tcatggtgtc ggcaatagtt tcatagccgc cgaggtaacc gaggtacgcc aggaaagagt
     2701 ggctgccgtt cagcatacgc agcttcatca tttcgaacgg cacaacgtct gcaacgaact
     2761 gtgcgcccac tttatcccag tccgggcgac cattaacgaa gttatcttca atcacccact
     2821 gacggaacgg ttcgcaggca atggcgcacg ggtcgtaaac acccagctgg tcagcaattt
     2881 cctgtaaggt ttctggcgtc gccgccggaa cgatgcggtc aaccatggtg catgggaagg
     2941 tgacgttctc ttcaatccat gccgccagct gcggatcgcg tgcctgagcc agtcccagta
     3001 ccgcgacctt cgccacatga ccgttttcac gcacgttatc gcaggacatc accgtaaacg
     3061 ctttcaaccc tttttcacga cgcagacgca aggcttcgac gatgtaacca atcgcggact
     3121 tcggcgcagt cgggttttcc agatcgtgct tgatcagtgg gttattgaga tccagttgac
     3181 cgcttgccgc atcagcgcag tagccttttt ccgtgaccgt tagagagaca atcgccgttt
     3241 gcggacgcgc catcgcgttg agaatacctt cgcagccatc gatttccgga tgcagcgctt
     3301 ctttcatcga accgataatt ttcagctcgg tgctctctgc gcctttttcc gctacggtgt
     3361 acagcagttg ctgttttttc aggttttcga tcagcacgcg gtcgttgcct ggcatcaggt
     3421 taacttcgca gatgccccag tcgctgtcgg tgctttccag cagatgatgg gtatacagcg
     3481 cctggtgcgc gcggtgaaac gccccgcaac cgagatgcac aatgcgtgat tccagacgag
     3541 aatgatccca tgacgggcgg gcaaccggca gattgctgtc aacaatagta gtcatcgtaa
     3601 actccagaat ccgtcttcaa agagtgacgg caaggaagaa ggaaccggca cggcgcgcag
     3661 ccatgccggt ggatattaac ggctaaagaa agcgcgctgg atcgccagtt cgacaccgcg
     3721 aacttcggcc aggcctttca gacgaccaat tgcggagtaa cctgggttgg ttttcttctt
     3781 caggtcgtcc agcatctgat gaccgtggtc cggacgcatc gggatcaggt cttctttgcc
     3841 ttccgcttta cgacggtgtt cttcttcaac aatcgctttc accacttcgt acatatcaac
     3901 gtcaccgttc aggtgcgccg cttcgtggaa ggttttcggg ttatcttcac gcatggtgga
     3961 gcgcagatgg gtgaagtaaa tacgcggacc gaactgcttg atcatatcaa ccagatcgtt
     4021 gtcagcacgc acgccgtagg aaccggtgca catggtaaaa ccgtttgcca tgctgtttac
     4081 ggtatcaacc atccactgca tatcttcaat ggtggaaaca atgcgcggca ggccgaggat
     4141 cgggcgcggc ggatcgtccg ggtgaacagc catacgcacg ccaacttctt cggcaaccgg
     4201 aataatcgct ttcaggaaga cggcaaagtt ttcgcgcagt ttggctttgt cgatatcttt
     4261 gtacagctcc aggtgcttac ggaactggtc gagggtgtac ccttcttccg cgcccggcag
     4321 accagcgatg atgttacggg tcagacgcgc tttgtcttcg tcgctcatag tggcgaagcg
     4381 ttcagctgcc tgagcaattt cttcttcggt gtaatccgct tccgcgcctg gacgtttcag
     4441 gatatgcatt tcgaatgcag cgaattcgat ctggtcgaag cgcagagctt tggagccgtc
     4501 tggcagcacg tattcgaggt cagtacgggt ccagtcgagc accggcatga agttgtagca
     4561 cacggtgcga atgccacact gcgccaggtt gcgcagagtc tgctgatagt tagcgatcca
     4621 ctgctcatag ttgccagtgt gggttttgat atcttcgtga attggtacgc tttctacgac
     4681 agaccacacc aggcctgcgt cttcaacgat cgccttgcgt ttgaggatct cttctacgga
     4741 ccatacttcg ccgttcggga tatggtgcag cgcggtaacc acgccagttg cgcccgcctg
     4801 acggacatca gctaaagaaa ccggatcgtt tgggccgtac cagcgccagg tctgttccat
     4861 gatttttcct ctttcacata gtgggttcca cagcaaagag acgcggaacc ctggagttgt
     4921 ttaggtatcc ggatgaacaa ccagaccgca gccgtggcgt tgcatcggat gagatggcgt
     4981 ataagttcta ccttttggta agacatgtca acccgaattc tgaaattggt taaccacatc
     5041 acaagaattt tactctcctg gttgttgctg caaattgcag caaagggtaa tcgatggtta
     5101 ttgatcagag gatgtgacat tcatcgcaac aatggttgac caatttacat aacatgtcgc
     5161 ccaaaataac aacggttcaa ccaggtaagg taaaacgtta tgcatgtgct taacattctc
     5221 tgggtggtat tcggcattgg tctgatgctg gtactgaatt tgaagttcaa aatcaattca
     5281 atggtggctt tgttggtggc ggcgctgtcc gtcgggatgc tggcgggcat ggatttgatg
     5341 tcgctgctgc acaccatgaa agcgggcttc ggcaacacgc tgggggaact ggctatcatc
     5401 gtggtgttcg gtgcggtcat cggtaaattg atggtcgact ccggcgcggc tcaccagata
     5461 gcgcatacgc tgctggcgcg tctcggtctg cgctatgtac agctgtcggt gattatcatc
     5521 ggcttgattt ttggtctggc gatgttctat gaagtggcct ttatcatgtt agcgccgctg
     5581 gttattgtta ttgccgccga agctaaaatt ccgttcctga aactggcgat cccggcagta
     5641 gcagctgcca ctaccgcaca ttcactgttc ccaccgcagc cgggtccggt ggcgctggtg
     5701 aatgcttatg gcgcggatat ggggatggtt tatatctatg gcgtactggt gacgatccca
     5761 agtgtaatct gcgcaggtct gatcctgccg aagttcctcg gcaatcttga gcgcccaacg
     5821 ccatcattcc tgaaagcaga tcaaccggta gatatgaata atctgccctc tttcggcgtt
     5881 tcgattctgg tgccgctgat cccggcgatc attatgatct ctaccaccat cgccaatatc
     5941 tggctggtaa aagatacccc tgcctgggaa gtggttaact ttatcggttc ctcgccgatt
     6001 gcaatgttta ttgcgatggt ggttgcattc gtactctttg gcaccgcacg tggtcatgac
     6061 atgcagtggg tgatgaacgc ttttgaaagc gcggtgaaga gtattgcaat ggtgattctg
     6121 atcatcggtg cgggtggcgt gctgaagcag actatcatcg acaccggcat tggcgacacc
     6181 atcggcatgt tgatgtccca cggcaatatc tcgccctaca tcatggcatg gctgatcact
     6241 gtgctaattc gtctggcgac gggtcagggt gtcgtttcgg cgatgaccgc cgccgggatt
     6301 ataagtgctg caatccttga tccagcaact ggtcagctgg ttggcgtgaa tccggcgctg
     6361 ctggtactgg cgacggctgc gggttccaac accctcaccc acattaacga tgcatctttc
     6421 tggttgttca aaggttactt tgacctgtca gtaaaagaca cgttgaaaac ctggggtctg
     6481 ctggagctgg tcaactccgt ggttgggctg attattgtgc tgattattag catggtagcg
     6541 taaaaggaca ccagagcctg ccaatggcag gctctgactg atgacaaagt ctaaaaatgt
     6601 gcccagacag tcctctcgcc cctctgggga gagggttagg gtgaggggaa aagaccagca
     6661 aagtgcgctt tgtttatgcc agatgcgacg ctaacgcgtc ttatctggcc tacaaagggc
     6721 taacgtgcag gtttttagct tcaggtaata ttgcgtacct gcattagcaa tgccctgtga
     6781 tttctttatt gataaacaaa agtcacgcca ataatcgatt gcacattccc tgcagtcacc
     6841 tgccctccgg tacgtgcgta atttgccgtt aatcccagac ttaccgccga agtccctact
     6901 gctcctaacg ataccgtgtt attcgctgga ataatcgtac cgttgcgcgt caactgtacg
     6961 ccgacgccct gtgcaggtga aaacgacgcg gtattggtga aaatcgagtt gcccgcatct
     7021 gcggttgtgc cggagaggta ataccccagg ttttggcttt tcgcacaata aacggtaaga
     7081 ggaattggca ctgaaccagg gtagtccggc agagtaacgg tgacatcacg agcagaaaca
     7141 tcgcagccgc cagtaggcac caccacatca ttattggcgt aaatattcca cacaaactgg
     7201 aaatcatcgc tgttatagtt gttggtctgt cgcaaaataa gcacggcaat taatgagcca
     7261 gctttaatcg ccaccccgcc cgcactgctc acaggcgtca aataaagcac caccggccac
     7321 ggcttatccg ttctcgaatt ataaacaacg cgcggcgttt cgctggtggt aggaaatgga
     7381 tagctactgc cactatattt tacggtcccg gaaaaattag ataacacgcc gccataagcc
     7441 gagcctcgtt gcagtgtgac atagtctgta atggtttccg gataatcgtt atggcaaaag
     7501 atttgcgtcg aaagatccac gaccaggttt tgccccacat tcacggcggg cgcaaggttt
     7561 acataaacat tggcgctgcc accgccaata gggatagcgg taccattggc ggttttacag
     7621 gcgaatgacc aggcatttac cgaccagccc atcagcagta cagcaaacag ggtaataact
     7681 cgtttcatta caatcatctc ttcgggttca gctgtaggta taggtgatgc taatcactgc
     7741 ctgaatggtt ccctgagtgg ctccgccatt taccgtcaat gctctgacct gtaacgggaa
     7801 gtgcgctgat tgtgaggaat catccacctg aactgttttg gttgcgccag tattcaatgt
     7861 gttgccactg tcatcctgta gctctaactg gatgttttgc gcggtcccct ggtttttata
     7921 atatccggta ctgtcggctg ccccgctgaa gctggcagtg accctcgacg ttcccaccgg
     7981 acaattagtc aactcaagcg caacatcatg ccaggccgat gccgccccgg cagacatcag
     8041 actgaaagaa taaagatcgc cgagatcaac cgtggcattg gtggtggaaa ctgtgcacgg
     8101 tttggcgacg accttaccgt tcaccgtgat ggtgacatcg gctgcctgta tcgtcgcact
     8161 tgcgagcgcc aatattgccg ccaatacata cccacgtttg caccatttca tgagcatctc
     8221 cagttactga tattcaagag tgaaggtagc cgtggcattg atatgccctg cagtgacagg
     8281 cacctgtgtc gccattagcc gggcgtaaaa attcagcgta tttggtttac ccggcgtcag
     8341 ggtcgtccac gaaagcgcgg acgatggagc attaaggggt atttgatttt gctgctcatt
     8401 cagaagctgt attcccagtc ccgaagccgc tgacaccgta ttttcaagtg caagcagatt
     8461 ggcattgtgg ctatctgcaa cgccggtaaa cccaactttt acggcagaaa cggcattacc
     8521 acagggtgac agcaaaatac gaaatggaac aacaggagtc gtcgcgccaa tgttgttaaa
     8581 ttgcttcgcc gcgttttcca tcagatcaac agtaaaattg gttgattcag cggccacact
     8641 acagccgtta tccctgacat agccgcggat agtaatcgtg ctatccgcag ccaaagcgtg
     8701 actcaccgcc agccacaaaa aagcgcacag aagataaaaa ggtttgtttc tcatcacgcc
     8761 cccttaacga cattcagctg atagctgggt taataactgc tgctgactct ctggtggcag
     8821 ttgataattg gcgacacagt gagcattttc ctcttctccc catttcacct gaacttttcc
     8881 cgctaaaggc attccgctga ggtaaacctg accattatcc gcaacaatgc cgctactctg
     8941 gctactctct gatgtcacca tcgccccaaa cggcagcggc ttattattgt gggtcagcgt
     9001 catgagcagt tttatcccaa cgcgcgcttt aaactctgct cgcacgatcg ccccacgagt
     9061 gggaacaacg ttagcaaccg cgttatctaa atcgacgtta tcagccaggg tattggtatc
     9121 cagcgccact ctattttccc gatattcagt ggcataaggc agcacggcat aaccacgcca
     9181 gtcggtacgc acccccgtct ggttttcgac ttttgcatct tttgcgccag gcgctttaac
     9241 aagcaccact gtttcgttta acggctgccc cagcgttacg ccattggcat gagccaatac
     9301 cccaccgctg actccgtaat agagctgctt aatatcatcg ctatggctgt aaccgatatt
     9361 ggcattgccg taaccaccgc gataattcag cgtggcgtag cctgtacttc cgctatttcc
     9421 atcgcctccc ccggcatagc cggtttgcac gctatagctg aggttgttgt cttccagcaa
     9481 cgtaccgtat acaccagcca gattggtcat ccgaccgttg agatcgtgtg acatgctgta
     9541 gctggcactg gcatgtcgcc actgagattt actgtcagaa cgcagccagt ggctgaaagg
     9601 aatattgacg ttaagcgcta acatctgatc ccgtcctttt tgccaggcgt ttttcgtcag
     9661 gctatagctg agcgtccagt tgatatcttc gaatgcagta tttaaaccag cctggaattg
     9721 ctcatcgaca ttactcgttc cccaataagt ttgatggcta ccactcaaat acagtgttga
     9781 tgtgcgcccg agttgctgag taacggtgag ctgtaatttc ccgcgtttgt tataagcgag
     9841 gttgtaatag tcggtgaatt tcggcttaac ctgaataact ccgtcctgtg tttcgatgtt
     9901 gtagccattc attcgactgt atgttgtatc agcgaaatta aaatatccgc tggtcgaata
     9961 acggtaaccc actaactgaa tattcgtgcc tgactcattg agcgatttgt tatagagaaa
    10021 acgcaccgat tgtccgtcat gctgactgtc atcgggaagt gtggaattag cctgcgtcat
    10081 atccacagac agagcgccca gtgcccccat gtttttcccg ataccgaaat taaaagcacg
    10141 ataacgatcc gccagttgcg ttccaccata tattgtccag ccagccggaa ggccgtggag
    10201 taatgtactc tggaaaaagc ggggtttttc ctgctgcgca tttccactac ggtattctcc
    10261 tgccgtaatg gaataacgag tatgcccttc acgttgcaaa agcgggactg acgaataggg
    10321 tacgataaaa atctgcgtgc tgccgtcagc ctctttgatc gttacctgca agtcaccact
    10381 attacctgcg gcatagatat cgttgatggt aaaaggcccc ggtggcaccg tactattata
    10441 aatgtcatac ccattttgtt taatagtgac ctgtgcagta ccacgagcaa taccgtggat
    10501 caccggggca aatcctcttt ggctatcggg taacatattg tcatctgagg ccaattgtgc
    10561 gccgcgaaag ttaataccat cgaaaatatc gccctgagta taaccatcac ccagcgtcag
    10621 ccgggaacgt aacggtatta tgtctcgctc aagccaggta ttgatatgct gccatttatt
    10681 tttgctacct gatgatctgt cgctacggtt ataactccag gtggtattgt cgcgtaaacg
    10741 ccacgcacca atatttaacc cactctgtag gtttaaatat gcataatggc tgttaccccc
    10801 aatccgattc tgtacactat ttccgctgaa attataattg agcaatccgg cattaatacc
    10861 gggatcccat aactcaggag gaatataacc acgcgcgcga ttactcataa atgcctgagg
    10921 gatcgtcagg ttgagtcgct gctgaccaac atctaaatgc gcagtagcgt catggattat
    10981 tgaggttaat ggcacgcagg catcatccgc cagcagattc ataccggaga cagaagccgt
    11041 attcagcccc atactggcaa gttgcgcgcg tgtcaggcag ggaacaatcc cttgttcgct
    11101 gtcgcccgta ttaaatgtga catcacgcgt tgccatataa ccattattca aatagatatc
    11161 gacacgatac gtccctggcg gtaattcttg cccattttca aaacgcgata aatcagccac
    11221 agcctgggga tcatccgcta aaaagcgcgg attaaaatag agttcggcag atgacaaagg
    11281 tgtctgtgcg gcaaaagcac aggcgacaaa gagccggaca aaaaaaccag ccaaacgatg
    11341 cttacgaata tgcaagcatt gtgtgtttcg ctggtaaagt cttaaattca gatatgacat
    11401 tattcctccg gtttaaaatc acccggcagt caattctttt tattcaggcg aaaaattccc
    11461 cctgcgttat tccattacgc ccgtcatttt gggggtaagt gcgccataat catttattgt
    11521 tcggtaagta atattgcttc ctgcatcaga aggcaattta accgtgcttt cgcccattgg
    11581 aggcaccaat gcattttcaa gaacccgggt tccggcattc aactctgtta ccgtcaggta
    11641 atagggtgtc gggttaatca gcgtcaggga attcgcgcta cgacgaaatc ttaatttttc
    11701 tgcggcctga tcgggtggca acgctaattt agccggacga tagtacagtt taatgcggct
    11761 gataattgcg agctgtagcg tattctcagt caattttgat ttatccattg acggaatcgc
    11821 tttaacattc atccagaata aactttcccg atcctgtggc aattggttat tagttgcatc
    11881 aagaatacgc aaggtattct ctctttttcc cttcatcgca aacagaggag gcgtcacgat
    11941 aaaacgacca tcctttacac catcggcatt ttccacccat gattgaatta aataggtact
    12001 attttcatca ttatttgtca cggcaagttg cacttgtttt tgccctgccg gataaattac
    12061 gcgagtcgca cctaaggcca ctcccgcttc agcgcgtccg gcaaccatca ttgccatgaa
    12121 catcaggata cctgccagca agcagaatgt tatttcctgc gatttcctta cattgacgtt
    12181 tttattactc actgtcctgt tcctgttatc acattatctg ctgaacaatt actgataggt
    12241 taaagagaac caggcctgtg cattggcgat gccgccagta acccgacgcc cggtagcacg
    12301 atatttggcg atgaaatgta gcgaagtaga gcctgagtaa agccgtttcc agtttgctgg
    12361 aggacgatta atcggtacga ggtttccttc atcatcaaac aacgctacgc caatattggt
    12421 ggctatccct ggcccctctc caacggaaag cacatctgga tttttaccat ccgcgacacc
    12481 gtgaaacgcc acacccacat gttcactcac caccgtgcta cattcccgta aatgaataac
    12541 aaaaggcacc ggtgcgctat cttccccaac cgcatgaaac cggttactgc tgatttgccc
    12601 catattgacc gtcatttgtt tatcaccggc ttcaatccgg caagtttccg caataatgac
    12661 gccctgaaat tgcatatttc cgccgggcaa cgtggtattc catttatttc cggccagagc
    12721 aaacattggc agcaacgacg ctaatagaaa taatctcttc cttttcatcg ttatttttat
    12781 cgcacaaggg tgggcatccc tgcccgtaat gacgtccctg aacctgggta ggttattgat
    12841 actgaacctt gaaggtcgca tccgcattag ctgcgccagg ggttgcggca ccggttgcaa
    12901 aataacgcgc ctggaacgga atggtgttgg ttccgttatt cagggttgtt tctgaactaa
    12961 atgtcgcacc gtccagcgcc agcgcagcac ccgttctgtc caggatctgc acaccaacgt
    13021 ttgttgcgct acccgcagct gaactctgca gagccagtac atcagtatgc gtagcatcaa
    13081 ttgccgtacc taagaaggca acagcggctt ttgtggcaac agtggtatcg caatcattca
    13141 gctgaatgtt aaaaccaacg gcagagctgg ttgctccagc ctgcttcagg ctagcggtac
    13201 gaacctggcc caactgaacg gtttgatcaa cagagcctgc atcaactgcg caagcggcgt
    13261 taacaacttc ccctttaaag tgaacggtcc caccatttac cgtcgtagta tcggccagag
    13321 ccgctgcgga actgagggac agagccgaca gaacaacgat tgccagagtt ttaattttca
    13381 tgctgctttc ctttaaaaaa actatttcta aatcgacatg ggcagtcgtt ctgtacactt
    13441 tgttttgtca acgagtttga ctgccaacac tgcacagttt tcccccaaaa gatgaaacat
    13501 ttggggccat tttgactcat agaggaaagc atcgcgcaca aactttttca gtttatttgc
    13561 tggcttaata ttctattgtt atctttattt atagattttt atattgcatg aggtggtttt
    13621 tggagagaag aatgaggaag atgcgtcgag ccacagaaac gttagcttta catatagcgg
    13681 aggtgatgtg aaattaattt acaagagaaa taatttacat atcaaacagt tagatgcttt
    13741 ttgtcgtttt ttaatatttt tatgcttgag aaaaaatacg taacttattt atgatatgga
    13801 cagtttggcc ccaattgtct tgtatttatt tgttttttaa ctttattatc aatgagttaa
    13861 atcaaacctc ttctcttttt aatttttcgt ttatgagatt atttctttcc catagtccgg
    13921 caaaacgagc agcattactg gcggtataac gtacagtatg gcgaatattt cgatgcccga
    13981 gataatcctg aattaaacgg gtatcggcac cacgctccgc cagttcataa ccacaagcat
    14041 gccttaacat atgaggatga gtctgcgtta cggttccagc ttcaataccg gcatcgcgaa
    14101 taatgcgata ggcctgctgg cgagaaagcc gactcccgcg gcgagaaata aatatagcgt
    14161 cggtccggtc agcgcctttc cagttagcac gttcctgggt ccagcgttcc acggcttcac
    14221 gctcatcaaa acgtaacggg tgaacggtag aaaatccgtt cttcagtcgg cgaatattta
    14281 ttcgaccttc attaaggtca agatcctgat aatgcagatc aagcagttca ctaatacgca
    14341 tcccatgccg atatgccaac agaataagac aataatctct ggctcccgtt gccccgtaac
    14401 aaaccgcctg catcatggcc tgaacttctt taccggtaag ataacgacgt ttactcacaa
    14461 tagtagtact cctaactgag atatatttga atgaatacct ataggaaacc tcaatcggtc
    14521 aaaattagcc caatagataa aaagataacc acacacgaag tgatgtggct atcagtcaat
    14581 tacattaaat at
//
LOCUS       DAZGAN020000119        14587 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGAN020000119 DAZGAN020000000
VERSION     DAZGAN020000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.119"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..167)
                     /locus_tag="ACHKYO_RS20025"
                     /old_locus_tag="pgaptmp_004005"
     CDS             complement(<1..167)
                     /locus_tag="ACHKYO_RS20025"
                     /old_locus_tag="pgaptmp_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HGU4430767.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTP"
     gene            complement(130..519)
                     /gene="yeaR"
                     /locus_tag="ACHKYO_RS20030"
                     /old_locus_tag="pgaptmp_004006"
     CDS             complement(130..519)
                     /gene="yeaR"
                     /locus_tag="ACHKYO_RS20030"
                     /old_locus_tag="pgaptmp_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HGU4430768.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNHGQSNVYRDRHQ"
     gene            complement(692..1330)
                     /gene="leuE"
                     /locus_tag="ACHKYO_RS20035"
                     /old_locus_tag="pgaptmp_004007"
     CDS             complement(692..1330)
                     /gene="leuE"
                     /locus_tag="ACHKYO_RS20035"
                     /old_locus_tag="pgaptmp_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HGU4430769.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1457..2380)
                     /gene="dmlR"
                     /locus_tag="ACHKYO_RS20040"
                     /old_locus_tag="pgaptmp_004008"
     CDS             complement(1457..2380)
                     /gene="dmlR"
                     /locus_tag="ACHKYO_RS20040"
                     /old_locus_tag="pgaptmp_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HGU4430770.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2483..3568
                     /gene="dmlA"
                     /locus_tag="ACHKYO_RS20045"
                     /old_locus_tag="pgaptmp_004009"
     CDS             2483..3568
                     /gene="dmlA"
                     /locus_tag="ACHKYO_RS20045"
                     /old_locus_tag="pgaptmp_004009"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HGU4430771.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASSEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            3819..5429
                     /gene="yeaV"
                     /locus_tag="ACHKYO_RS20050"
                     /old_locus_tag="pgaptmp_004010"
     CDS             3819..5429
                     /gene="yeaV"
                     /locus_tag="ACHKYO_RS20050"
                     /old_locus_tag="pgaptmp_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HGU4430772.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            5461..6585
                     /gene="yeaW"
                     /locus_tag="ACHKYO_RS20055"
                     /old_locus_tag="pgaptmp_004011"
     CDS             5461..6585
                     /gene="yeaW"
                     /locus_tag="ACHKYO_RS20055"
                     /old_locus_tag="pgaptmp_004011"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HGU4430773.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            6641..7606
                     /gene="yeaX"
                     /locus_tag="ACHKYO_RS20060"
                     /old_locus_tag="pgaptmp_004012"
     CDS             6641..7606
                     /gene="yeaX"
                     /locus_tag="ACHKYO_RS20060"
                     /old_locus_tag="pgaptmp_004012"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HGU4430774.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(7660..8775)
                     /gene="rnd"
                     /locus_tag="ACHKYO_RS20065"
                     /old_locus_tag="pgaptmp_004013"
     CDS             complement(7660..8775)
                     /gene="rnd"
                     /locus_tag="ACHKYO_RS20065"
                     /old_locus_tag="pgaptmp_004013"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HGU4430775.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(8857..10542)
                     /gene="fadD"
                     /locus_tag="ACHKYO_RS20070"
                     /old_locus_tag="pgaptmp_004014"
     CDS             complement(8857..10542)
                     /gene="fadD"
                     /locus_tag="ACHKYO_RS20070"
                     /old_locus_tag="pgaptmp_004014"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HGU4430776.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(10747..11328)
                     /gene="yeaY"
                     /locus_tag="ACHKYO_RS20075"
                     /old_locus_tag="pgaptmp_004015"
     CDS             complement(10747..11328)
                     /gene="yeaY"
                     /locus_tag="ACHKYO_RS20075"
                     /old_locus_tag="pgaptmp_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HGU4430777.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADMN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(11368..12063)
                     /gene="tsaB"
                     /locus_tag="ACHKYO_RS20080"
                     /old_locus_tag="pgaptmp_004016"
     CDS             complement(11368..12063)
                     /gene="tsaB"
                     /locus_tag="ACHKYO_RS20080"
                     /old_locus_tag="pgaptmp_004016"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGU4430778.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPEIVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12121..14031)
                     /gene="yoaA"
                     /locus_tag="ACHKYO_RS20085"
                     /old_locus_tag="pgaptmp_004017"
     CDS             complement(12121..14031)
                     /gene="yoaA"
                     /locus_tag="ACHKYO_RS20085"
                     /old_locus_tag="pgaptmp_004017"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HGU4430779.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14163..14507
                     /gene="yoaB"
                     /locus_tag="ACHKYO_RS20090"
                     /old_locus_tag="pgaptmp_004018"
     CDS             14163..14507
                     /gene="yoaB"
                     /locus_tag="ACHKYO_RS20090"
                     /old_locus_tag="pgaptmp_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU4430780.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3528 a         3870 c         3621 g         3568 t
ORIGIN      
        1 ggggttttac ggcgcaccgg ataagaacgc acggtattca ccagatcttt tatcacttca
       61 gccgccactt ctggcaccag caaagtcatc ggcgtctctg tttcataatc gacagaaacg
      121 ccattgctgt tattggtgac ggtcacggta tacgttgctt tgcccatgat tatgaatgac
      181 tttccgttgt tgcgcacctt ccatcaggac ttcaggagcc acgaagaagt caatgttgaa
      241 ataagtatcg tcagtcatgg cttcaatgtt gtgccacttt tctggaggga acaccgcaaa
      301 ctgccccgct tcgataagga tcacctgatc aggctctgca ctgtgttcat cagcgtagcc
      361 gagatatttg accgccccat gcataacgga aaggcgtggg taaacccccg ggcgcgttcc
      421 tttatcaaga tgacgttcga atattccggc aggcgcagtt tgtttattcc agaaaggcgt
      481 tgagcgcgta tgaatataat tctgtgggat ttgcagcatc ctattccctc cttcggggaa
      541 tgcgctgaaa acggtttatt ccagccgttt cagggtacgc ctgataattt gcattttaaa
      601 taccatttat tggttacttt ttagcaccat atcagagaaa aatcagggag gattacaagt
      661 gggaagcccg cgacaacgcg ggctgaatgt atcaggattg cagcgtcgcc agtcgggcgg
      721 cgaaacccac gaacatcaaa ccaatcagtg agttgccaac tttagccagt ttctttttgg
      781 tacgtatgta ctgcgtgaca aaagcaccag aaataatcag gaagctcaaa tagcagaaac
      841 tcaccagttc cagcgtcgtc gccagaataa agaatgaaat tcccgtatgt ggggcattaa
      901 catcgataaa ctgtacgaaa aacgacacat agaacaaaat ggctttcgga ttagtcaggc
      961 tcaaaattaa cgcgcgttta aaaatggccc cgtattgggg ctcatcggat ttggcctcgt
     1021 tatttttacc cttcagggtc gcgtagagaa ttttactccc cagatagagc aaataaaacg
     1081 caccaagata acgtacaatg ttgaataata tcggggtggt cttaattaat gtcgccactc
     1141 cagcccatgc cagaaacatc aatactgcat cgccaataaa tacaccgcag gccgcaagat
     1201 aaccgccttt cataccgcta ctgacgctat ttttgagtac aaacagggta tttggccctg
     1261 gcaccaacac aataaaaatg gccccaacca gataggtcca gtaattcaga accccgtatt
     1321 cagcgaacac attaacctct ttaattatct ttcgatcatg cgcgattaaa ggtgaatatg
     1381 ctaaccaatc tgtagcggct tagaaaggag aaaatcaggt tttaacccga tatcaacccg
     1441 ataattgaat cattaatcag gcatgctcca gtgaaaaatt cgggtagtgc tcggcaaaat
     1501 actggcgtaa aaattctacc gttatccgca ctttcgctga cgtcgccagc cttgaaacat
     1561 aaacggccca gacgttcgct ggctggtaat attccggtag cacttgcact aaatgaccac
     1621 tggcaatgtt ttcgctaaca tcccaccagg agcgcagagc aatcccctgc ccatcgagac
     1681 accactgatg gacaatttct ccatgattag acgacagtgg gccggtgact ttaatcgcat
     1741 ggtggccttc tttattgcgt aattgccaga caccaaaagg atgatcgcgc tctttaatga
     1801 ccaggcacgg caatgcggaa agatcggtta aatgtttcgg cgcgccatgt tgcgcaataa
     1861 attccggcga cgcgcagaga atgcggtaat tagtcgccag tttgcgggcg attaaattag
     1921 gggcgatgtc atcgccaatg cgaatatcga gatcaacacc ttcattgacc aaatcgacca
     1981 accggtcttc cacatcaaaa cgtaattcca gttgcggata cgccttagcc agcgctgata
     2041 acgccggagc caccacctgt cgtccaaaac caaagctgct gataatacgc agcatcccct
     2101 gcggcacctg acgcacgtca gaaagttcgt ccatcatctg accgacatcc tgcaaaatcc
     2161 gctgcgccca ttcataaatc cgctctcctt cttcggtaat ggtgacgcgg cgggtggtgc
     2221 ggtgtaacaa caccacgttt agcgtttgct ccagcaaggc gatgcgcttg ctgacgaacg
     2281 ccggtgaaac gcccagttct tccgccacgg cggcaaaacc agcccgacga gccaccagca
     2341 taaagacgcg caaatcattc agcagcggta aattattcat gattcgtgtt ttatgtttca
     2401 ccagttacgg ggattaattc cttttcagtc aattatagga tggtgatgtt gtcaattttg
     2461 atggtcagga agtgagaacc caatgatgaa aacgatgcgt attgctgcga tcccgggaga
     2521 cgggattggc aaagaagtcc ttcctgaagg gattcgcgtg ttacaggctg ccgctgagcg
     2581 ctggggcttc gccttgagtt ttgagcaaat ggagtgggcg agcagcgagt attacagcca
     2641 tcacggtaaa atgatgccgg acgactggca tgagcaactt agccgtttcg acgccatcta
     2701 ttttggtgcc gtcggctggc cggacaccgt tcctgaccat atttcgttgt ggggttcgct
     2761 gctgaaattt cgtcgtgaat tcgaccagta cgtcaacctg cgcccggttc gtctctttcc
     2821 tggcgttccc tgcccgctgg cgggaaaaca gcctggcgac atcgattttt acgtggtcag
     2881 ggaaaacacc gaaggcgaat attcctcgct cggcggtaga gtgaatgaag gtacagagca
     2941 tgaagtcgtc attcaggaat cggtatttac gcgtcgtggt gtcgatcgca ttttgcgtta
     3001 tgccttcgaa cttgcgcaaa gccgcccacg taagacgcta acttctgcca ctaaatcaaa
     3061 cggtttagcc atcagcatgc cgtactggga tgagcgagtg gaagcaatgg ccgagaatta
     3121 cccggagatc cgctgggaca agcagcatat tgatattctc tgcgcgcgtt ttgtgatgca
     3181 gccggaacgc ttcgatgtgg tggtggcgtc caatttattt ggcgatatcc tttccgatct
     3241 tggcccggcc tgcaccggca ccattggcat tgccccatcc gccaacctga atccggaacg
     3301 cactttcccg tcgctcttcg agccagtcca cggttccgcg ccggatatct acgggaaaaa
     3361 cattgctaac cctatcgcca cgatttgggc cggtgcaatg atgctcgatt ttctcggcaa
     3421 tggcgatgag cgtttccagc aagcgcataa cggtattctg gcagcaattg aagaagtgat
     3481 tgctcacggg ccgaaaacac ctgatatgaa aggcaatgcc accacgccac aggttgccga
     3541 cgcgatttgc aaaattattt tgcgttaagg tcaaaccagt ttatttgaac cgcgtcactg
     3601 acgcggtttt tttattcgtt ctttgcagta aataacctgc gtcatttcac cttttattgt
     3661 ttccgtttcg tgttttatgg ctttccgtat tcttaattgt ttaatttatg taacatgcaa
     3721 ataatcagtt tgttaaacac aacatacaac tcattaccct gcaaataatt acaactggca
     3781 aaagccagag acgggctttt atgcccagga gatgactcat gatgagcaat gttaagaaaa
     3841 aagatgtgcc gctgataagc atcagcctgg tggccattct tttcatcgca gctgcattaa
     3901 gccttttccc acaacaatcg gccgacgcgg ccaacgcaat atacactttt gttacgcgta
     3961 cgttaggttc cgccgtacag gtattggttt tgctggcaat gggactggtg atttatttag
     4021 ccaccagtaa atacggcaat attcgtcttg gcgaaggaaa accggaatac agcacgctct
     4081 cctggctgtt tatgtttatt tgtgccggtt taggttcttc tacgctttat tggggggttg
     4141 ctgaatgggc ctattattat caaacgcctg gattaaatat cgcaccgcgt tcacaacagg
     4201 cactcgaatt tagcgtccct tactccttct tccactgggg catcagcgcc tgggcaactt
     4261 atacgctggc ctcattaatc atggcttatc actttcatgt gcgtaaaaac aaaggtctga
     4321 gtctttccgg cattattgcc gcgattaccg gcgttcgccc gcaaggccca tggggaaaac
     4381 tggttgattt gatgttcctg atcgccactg tcggcgcact gactatttcc cttgttgtca
     4441 ccgcagcaac ctttactcgt ggactttccg cgctgaccgg tttacccgat aacttcaccg
     4501 tgcaggcatt tgtgatcctg ctttccggcg gcattttttg cctaagctcg tggattggta
     4561 tcaacaacgg tttgcaacgt ctgagcaaaa tggttggctg gggcgcgttc ctgctgccat
     4621 tactggtgct gattgtcggc ccaaccgaat ttattaccaa cagcatcatc aatgccatcg
     4681 gtctgaccac gcaaaacttc ctgcaaatga gcttattcac cgatccgctt ggcgatggtt
     4741 catttacccg caactggacc gttttctact ggctgtggtg gatctcatac acccctggcg
     4801 tagcaatgtt tgtcacccgc gtttcccgcg gtcgtaagat taaagaagtt atctggggac
     4861 tgatcctcgg cagcaccgtc ggttgctggt tcttcttcgg cgtaatggaa agctatgcca
     4921 ttcatcagtt tatcaatggc gtaatcaacg tcccacaggt gctggaaaca ctgggcggcg
     4981 aaacagctgt acagcaagtt ctgatgtcgt tgccagccgg taaattgttc ctcgccgcat
     5041 acctgggcgt gatgattatt ttccttgcct cgcatatgga tgcggtggcc tacaccatgg
     5101 ctgcgaccag tacgcgtaat ctccaggaag gtgacgatcc tgaccgtggg ctgcgtcttt
     5161 tctggtgcgt ggtgatcact ctgatcccgc tttccatctt gtttaccggt gcttcgctgg
     5221 aaacgatgaa aaccaccgtc gtgctcacag cccttccctt cctcgtcatt ttactggtga
     5281 aagtcggcgg atttattcgc tggctgaaac aggattacgc cgacattccg gctcatcaag
     5341 ttgaacatta tctcccgcag acaccggttg aagccctgga aaaaacacca gtgctccctg
     5401 cgggaaccgt attcaaaggc gacaactgag cgccatcatc ctaacgataa aggtatccct
     5461 atgagcaatc tgagccctga ctttgtacta cccgaaaatt tttgcgctaa cccgcaagag
     5521 gcgtggacca ttcctgcccg tttttatacc gatcagaacg cgtttgaaca cgaaaaagag
     5581 aacgtcttcg ccaaaagctg gatttgcgtc gctcacagca gcgaactggc gaatgccaat
     5641 gattatgtga cgcgtgagat cattggcgaa agcatcgtgc tggtacgcgg tcgtgataag
     5701 gttttgcgcg cgttctataa cgtgtgtccg caccgtggtc atcagttgtt gagcggtgaa
     5761 ggaaaagcaa aaaatgtgat tacctgcccg tatcacgcat gggcattcaa actcgatggc
     5821 aatctggccc atgcacgtaa ctgcgaaaac gtcgccaatt tcgatagcga caaagcgcaa
     5881 ctggttccgg tgcgtctgga agaatatgcc ggattcgtct tcatcaacat ggaccccaac
     5941 gccaccagcg tagaagatca gttacccggt ctgggcgcga aagtgctgga agcctgcccg
     6001 gaagtccacg atctgaaact ggcggcccgc tttaccaccc gcacgcctgc caactggaag
     6061 aacattgtcg ataactatct cgagtgctat cactgtggtc cggcgcatcc aggtttctcc
     6121 gactccgtac aggttgatcg ttactggcac accatgcacg gtaactggac gctgcaatac
     6181 ggtttcgcca aaccgtccga acagtcgttt aaatttgaag agggtacgga tgcggcattc
     6241 cacggtttct ggctgtggcc gtgcacgatg ctgaacgtca ccccgatcaa agggatgatg
     6301 acggtcattt atgaattccc ggtggattct gaaactaccc tgcaaaacta cgatatttac
     6361 ttcaccaatg aagagttaac cgacgagcaa aaatcgctga ttgagtggta tcgcgatgtg
     6421 ttccgtccgg aagatttacg tctggttgaa agcgtacaga aagggctgaa atcgcgtggc
     6481 tatcgtggtc aggggcgcat catggccgac agtagcggta gtggcatttc cgaacatggt
     6541 atcgcccatt tccataatct gctggcgcag gtgtttaagg actaatgaca tcggcggcgg
     6601 tattttccgc cgctgggctg atttttgatg gagtacagca atgtcagact atcaaatgtt
     6661 tgaagtacag gtgagtcagg tggaaccgct taccgaacag gtgaaacgct tcacgctggt
     6721 ggcaaccgat ggcaaaccgc tgcctgcgtt caccggagga agtcacatca ttgtgcagat
     6781 gagcgatggt gataaccagt acagcaatgc gtattcacta ctgagttcgc cgcatgacac
     6841 ctcttgttat cagattgccg ttcggctgga ggaaaactcg cgcggcggtt cccgcttttt
     6901 gcatcagcag gtaaaagtgg gcgatcggtt aacgatttca acgcctaata acctgtttgc
     6961 gctaattccc tcagccagaa agcatctgtt tatcgcgggc ggtattggta tcaccccttt
     7021 cctgtcgcac atggcagagc tgcaacacag cgacgtcgac tggcagctac attactgctc
     7081 gcgaaatcca gaaagttgcg catttcgtga tgagctagtc cagcatccgc aggctgagaa
     7141 agtccatttg catcattcat caaccggaac acgactggaa ttagcgcgat tattggcgga
     7201 tatcgaacct ggcacacacg tttatacctg tggccccgag gcgctaattg aagcggtaag
     7261 aagtgaagct gcgcgtctgg acatcgccgc cgatacgctg cactttgagc aatttgctat
     7321 cgaagacaaa accggcgatg catttaccct ggtgcttgcc cgttccggaa aagagtttgt
     7381 ggtgccggaa gagatgacta ttttgcaggt tattgaaaat aataaagccg cgaaagtgga
     7441 atgtttatgt cgtgaagggg tatgcggaac ctgcgaaaca gcaatactgg aaggtgaagc
     7501 tgaccatcgg gatcaatatt ttagcgatga agagcgtgcc agccagcaaa gtatgttgat
     7561 ctgttgttcg cgtgcgaagg gtaaacgcct ggtgttggat ttgtagtttg ccgaagccgg
     7621 atgtggcgct gagcgcgccc agtccggctt cggaagattt tactgcggat attcctgcaa
     7681 taaattgtgt aatgcttccg ccatcagctc accacgccag ccggaaatca gctccggcaa
     7741 attgttctgc ggtttcagtt tccagtgcca gttcagcagt tggttgattt gccgacgcga
     7801 tgccagcaat tcggcgctga tcttatgcgt ttcgctcacg tcagtaatca gcgacttaat
     7861 cgctttaaac gctttacgat aacccggcat gtccatcagg ttaagcatcg gctgcggtaa
     7921 ggcctcttcc ggcaatgtct gcgctttttc caccagcgcc agcagcgttt taccgtgaaa
     7981 gcggatttcg ctaccggata aacccaggct gtccagttcg cctaaacttc ccggcatata
     8041 acgcgctacc gaccacaaat gctcttcacg cacgacaaag ttcaccgcca gatcgcgctc
     8101 tcgcgccttg cgcagtcgcc agtcggctaa cagttgcaga caggccagtt ggcgtgtgcg
     8161 taattgccag gcattggtga tatcacgcca ggcatcttcc ggcgcaacga cttcctgacg
     8221 acgcatttgc atcaggcggc attcatccag cgccgcaggt agccagccgg aggcctccgt
     8281 ttctaccata agcttggcgg tgatcggtaa cagataccag acatccgccg ctgcgtattc
     8341 acactgacgt tcggtcagcg gtctggccag ccagtcggtg cgcgattcac tcttgtccag
     8401 cgtaacgccg gaatactctt ccaccatgga agcgaaaccc catgacatcg ggcgtccgca
     8461 gaaggcagca aggatttgcg tgtcaatcaa gggttgtggt aattcgccaa agacattgag
     8521 gaacacttcc agatcttcac tgcctgcatg gagaaatttt gtgatggacg gatcgcgcag
     8581 gatcgctttc agcggtgacc agtcggtgat cccgagagga tcaattagcg ccagatgctc
     8641 gccatcgaaa agttgaatca accccagctg cgggtaataa gtgcgcgtac gaacaaattc
     8701 agtatccagg gctatcgccg gaaaggcacg gacggcttca cacaaagaag ccagcgcatc
     8761 gtccgtggta atcatttggt aattcaaatt gttttctctt tagtgggcgt caaaaaaaac
     8821 gccggattaa ccggcgtctg acgactgact taacactcag gctttattgt ccactttgcc
     8881 gcgcgcttcg tcacgtaatt ctcgtcgcaa aatttttccg acgttagatt tcggtaactc
     8941 atcacgaaac tccaccagct tcggtacttt gtagcccgtg agctgacggc ggcaaaaggt
     9001 caccagtgac tcttcggtaa gcgatggatc ttttttcact acgaagattt tcaccgcttc
     9061 accactggag ccggaaggta cgccaacagc cgcgacttcc tgtacgccag gatgctgcat
     9121 gacgacatct tcaatctcgt tgggatagac gttaaaaccg gaaaccagaa tcatgtcttt
     9181 tttacgatcg acaatgcgca ggaacccttc ttcatccatc accgcaatgt cgccggtgtg
     9241 taaccagcca tttttgatga tctcatctgt agcatccgga cgctgccagt aacccagcat
     9301 cacctgcggt cctttgacac aaagctcacc cggttgaccc ggtggtactt cattatcatc
     9361 atcatccacc agtttggctt ccgtcgacgg caccggcaaa ccgatgctac cactatgata
     9421 atcaatatca tatgggttaa cgctgaccag cggcgcacac tcggtaaggc catagccttc
     9481 cagcagatac tgtcctgtca gtttcaccca acgctctgcc accacttgct gcactggcat
     9541 ccctccgcct gcggaaagat gcagactgga gaaatccagc tgctggaact ctttattgtt
     9601 cagcaacgca ttgaacaagg tgttaacgcc cgtgatagcg gtaaacggat atttcgctaa
     9661 ctcttttacc aaccctggaa tatcgcgcgg gttagtgata agcaggttct gcccacccag
     9721 ttcgataaac agcaggcagt taatggtcag ggcaaaaatg tgatacagcg gcagcgccgt
     9781 caccaccagc tctttgcccg gatgcaacag cggaccatag gtggcgttga cctgttccag
     9841 gttcgccagc atattgcggt gagtcagcat cgcgcctttc gccacacctg tggtgccgcc
     9901 ggtgtattgc aggaaggcta aatcttccgg caccagttcg ggtttgacgt actgcatccg
     9961 gtagccgtta tgcagtgcgc tacgaaatga aatggcatct ggcagatggt atttcggcac
    10021 caaacgcttg atgtatttaa caacgaaatt gactaccgtg ccttttgccg tagatagctg
    10081 atcgcccata cgggtcagaa ttacgtgctg aacggcggtt ttatcaacca ctttttccag
    10141 tgtgtgagca aagttagaca cgataacaat cgccgatgcg ccgctatcgt taagctgatg
    10201 ctcaagctca cgcggggtat acaacgggtt aacgtttacg acgatcatcc cggcacgcaa
    10261 aatgccaaac agcgccaccg gatattgcaa taaattaggc atcatcaacg caacgcgatc
    10321 gcctttcttc agccccaacc cttgttgcaa ataagcggca aacgcgcgac tgcgttcttc
    10381 cagcttgcgg aaggtcatta cctcccccat attcacaaac gcaggctgat cggcgtagcg
    10441 cgcgaccgac tgctcaaaca tatctatcag agattgataa cggtcagggt tgatctccgt
    10501 cggaacgtcc gcgggataac ggttaagcca aaccttcttc aattcttcac ctctaaaatg
    10561 cgtgttcgtc gtcatcgcaa ccccaaatga tatacattcc gttaacacaa tattaactca
    10621 tcataccagc ttgataatta cccaacgaaa aggttgcgaa gcgcgtcact atttattttt
    10681 atctttaccg taagaatgca gaaacagcgg accagccgct gtttcttttt ctttaaaaac
    10741 aagcaattat tcagttacaa ctgtttgtac tctcgcgggg ccgggattat accagcccca
    10801 tccgccatag ccatagggcc agccacgacc gccataaaac catggatcaa tcggctgagg
    10861 cggcataatc acctgctggg ttaaatgcca acgtttgtaa cccgttactt gcatcaccat
    10921 aaatttatag ggcgtgttgc cgatttttcc gtcaaccgca ccagtgattg gcccgactac
    10981 cgtaaccagt tgtccacgga aatccaccgg gtccagaaaa ccgttcatat cggcataaat
    11041 gcgaccgcga gaaggttctc ccagcgtcgg tctggctccg ctgtccagcg gtacggtagc
    11101 aatttccagg cgggttttcc cttgctggtt ttgtaccgca accactttgc caccaaagcg
    11161 tgcctcctga ccaacgtaca gctgcggcgc actcatcacc cgaactaaat cttgttgcgg
    11221 cgtgggactg ctgcctttaa tggcgtccgg cacagtgaca caaccgctca gcattagcgc
    11281 aaacgtacct gccagtatgc ctttgataac attcttttga accgccatgg tgcgactcct
    11341 ttttttctca gggcatactc ttaagattca ttctttgccc ggaagtttct tccatgcgac
    11401 gttgttacgt aaataaaccg gttcggcatg ttccaccgcc accgttttac cctcggcaaa
    11461 catctgacac gcaatcggca gcatatcttc agcagcaggc agtaacactt cgccatcgcg
    11521 caaaaccagc ccgctctctt taccgagatc cggccaggct tgccagcccg tccctaccgt
    11581 cacccattcg ccggaaagct gttgcattcg ttcatggacg atttcgggtt tgagtacggc
    11641 ttcggtttct tcaccgtgcc agataccgtt ttcatcacgc tgatattcgg cccagtaaac
    11701 ttcgcccatt cgcgcgtcaa tggctgacag cacgcgggtt gcgccgtttt tgcgccacgc
    11761 cccttgcgcc atcgtcatta gcgtggagac gccaatcatc ggtaattccg cgccaagcgc
    11821 cagcccttgc gcgatgccaa taccaatgcg cacgccggta aagctaccgg ggccgcgccc
    11881 gtaagccaga gcgtttatgt cagtcaggga agttccgctg gtggtcagga tatcctgcac
    11941 catcggtaag attcgttgag tatgttcacg agggcaaagc tcaaaatgag cgttgacagt
    12001 accgtcgttc cacagggcga cagagcaggc ctctgtcgcg gtatcgatag ccagaattcg
    12061 catgggtctt cgtgcttttt tcaataaaaa ggcgcgcatc ataccatact ccgtaacaaa
    12121 ttacctggag gatggtatcg caaggaaacg aaccgcacgg gcaatgtcac gggtgcgcgg
    12181 cgcgggcggc agactggcga gaaacgtcgc gccgtaaggg cgcatcacca gccgattgtc
    12241 acaaatcacc aaaaccccgc gatcgtcggc gtcgcgaatc agtcgcccta ccccctgctt
    12301 gagagtaatc accgcatccg gcagttgtac ttcatcgaac gggtcgccac cgcgcaaacg
    12361 gcaatcttcc atgcgcgctt ttaacagcgg gtcatccggt gaggtaaacg gcaacttgtc
    12421 gataatcacc aatgacaatg tatcgccacg cacgtccacc ccttcccaga aactactggt
    12481 tgccacaaga agcgcgttac cggcgctgac aaattgctgc aacagttgcc ctttgctggt
    12541 ttccccctgc aacaaaacgg gaagcgtcat ggtagcgcgg aactgctcgg ccagatcacg
    12601 catcatggcg tgcgaggtac aaagcataaa acaacgaccg ttgttagctt cgatgatcgg
    12661 tcgcagcatt gccgctaact ggcgagcaga acctggctgg ttggtttgcg gcagattgcg
    12721 cggcacacag agtaacgcct ggcggctgta atcaaacggg cttggcaata gcaacgactc
    12781 cgcctgttct atcccaagcc gcgaggtgaa atgatgcaga tcgtcgttca ccgacagcgt
    12841 tgctgaggtg aagatccagc taccaggctt ttgcgccatt aactctttga atttatccgc
    12901 cacgctgagc ggcgtgagag ccagagtaaa atggcgcgaa gtgcattcgt accagtagct
    12961 gtagcccggc tgattgatct cttttagccg cttcagccgt gtgcgataca acgtagcgcg
    13021 ctcaaatgcc gcatccagca aggcggaacg ccccagtgac agtttcgcca cgtcataaca
    13081 aagttccagg gtgtcatcga gcagtaaaaa tgcccgctga atttgcggat tagctaacag
    13141 ctcacgcagg ttaccgcgat aacctggctc accgagttgc agacgaaaat cctgcgcact
    13201 ctgggcaaga cgatcagcgc acttttgtaa ctgctgggtg tcttttaatt cggtgcggta
    13261 agcgatggtg atgtcttttg ccaggtcgag cagttgtcga ctggagagtg actgaccaaa
    13321 atactggctg gcaatgtccg gtagctggtg ggcttcgtcg aagatcatga cgtccgcttc
    13381 cgggatcagt tcgccaaatc cactctcttt aaccaccata tccgccagaa agagatgatg
    13441 gtttaccacc accacatcgg cgtccatcgc ttttttacgt gctttgacca caaagcaatc
    13501 tttatacatc gggcagtcgc tgccaagaca gttgtcgttg gtgctggtga ccagcggcca
    13561 cgcctgtgaa tcttccgcca cgctgacgca ggtgctgata tcaccatcga ctgtttgatt
    13621 agaccaggag cgcagcagga tcacatcgct taagatttgt accggcagat cgccccccgc
    13681 cagcgcctgc tgttcgagac gttcgaggca gaggtagttt gagcgccctt tcagcagcgc
    13741 cacgttgccc gtatatttca atgcctttga gactgttggc aaatcgcggc tgtagagctg
    13801 atcctgcaac gcttttgagc cggtcgagat aatgactttc tttttcgccc gcagcgcagg
    13861 agccaggtaa gcgtaggttt tgcccgtacc ggttcctgct tccaccacca gcggctggcc
    13921 tttttctatc gcctgggtga cggctaccgc catctgtcgc tgtggttctc gcggcttaaa
    13981 gcctggtatc gctttcgcca gctgaccgtc tggtgcaaaa tcgtccgtca cactaccccc
    14041 tgttgatttg aacagggatt atgtcaggat gagggcgctt tcgccagttg aagtggtgac
    14101 ggcgacctca cattgtggca gtctttgcag cacgaaaatg gaaggataat gaggacaaaa
    14161 tgatgactat cgttcgtatc gatgctgaag cccgctggtc tgatgtggta atccacaaca
    14221 acacgctcta ctacactggt gtaccggaaa acctcgacgc cgatgccttt gagcaaaccg
    14281 ccaacacgct ggcgcagatt gatgccgtgc tggaaaaaca gggcagcaat aaatcgagca
    14341 ttctggatgc caccattttc ctggccgata aaaacgactt cgcggcgatg aataaagcgt
    14401 gggatgcctg ggttgtcgcg ggtcatgcgc cggtgcgctg cacggtacaa gcgggtttga
    14461 tgaacccgaa gtataaagtt gaaattaaga ttgtggctgc ggtgtaagct ttatcgaagc
    14521 aaaataagtc agacgataat ttatcgataa tactggtcgg ttttacataa atcgaccaga
    14581 gattaag
//
LOCUS       DAZGAN020000120        14349 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGAN020000120 DAZGAN020000000
VERSION     DAZGAN020000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.120"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            46..1617
                     /gene="garD"
                     /locus_tag="ACHKYO_RS20095"
                     /old_locus_tag="pgaptmp_004019"
     CDS             46..1617
                     /gene="garD"
                     /locus_tag="ACHKYO_RS20095"
                     /old_locus_tag="pgaptmp_004019"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HGU4430781.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SSIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1766..2101
                     /gene="prlF"
                     /locus_tag="ACHKYO_RS20100"
                     /old_locus_tag="pgaptmp_004020"
     CDS             1766..2101
                     /gene="prlF"
                     /locus_tag="ACHKYO_RS20100"
                     /old_locus_tag="pgaptmp_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HGU4430782.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2101..2565
                     /gene="yhaV"
                     /locus_tag="ACHKYO_RS20105"
                     /old_locus_tag="pgaptmp_004021"
     CDS             2101..2565
                     /gene="yhaV"
                     /locus_tag="ACHKYO_RS20105"
                     /old_locus_tag="pgaptmp_004021"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HGU4430783.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2620..3429)
                     /gene="agaR"
                     /locus_tag="ACHKYO_RS20110"
                     /old_locus_tag="pgaptmp_004022"
     CDS             complement(2620..3429)
                     /gene="agaR"
                     /locus_tag="ACHKYO_RS20110"
                     /old_locus_tag="pgaptmp_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HGU4430784.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3678..4958
                     /gene="kbaZ"
                     /locus_tag="ACHKYO_RS20115"
                     /old_locus_tag="pgaptmp_004023"
     CDS             3678..4958
                     /gene="kbaZ"
                     /locus_tag="ACHKYO_RS20115"
                     /old_locus_tag="pgaptmp_004023"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HGU4430785.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            4981..5454
                     /gene="agaV"
                     /locus_tag="ACHKYO_RS20120"
                     /old_locus_tag="pgaptmp_004024"
     CDS             4981..5454
                     /gene="agaV"
                     /locus_tag="ACHKYO_RS20120"
                     /old_locus_tag="pgaptmp_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HGU4430786.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5465..6244
                     /gene="agaW"
                     /locus_tag="ACHKYO_RS20125"
                     /old_locus_tag="pgaptmp_004025"
     CDS             5465..6244
                     /gene="agaW"
                     /locus_tag="ACHKYO_RS20125"
                     /old_locus_tag="pgaptmp_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HGU4430787.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6234..7112
                     /gene="agaE"
                     /locus_tag="ACHKYO_RS20130"
                     /old_locus_tag="pgaptmp_004026"
     CDS             6234..7112
                     /gene="agaE"
                     /locus_tag="ACHKYO_RS20130"
                     /old_locus_tag="pgaptmp_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HGU4430788.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7130..7564
                     /gene="agaF"
                     /locus_tag="ACHKYO_RS20135"
                     /old_locus_tag="pgaptmp_004027"
     CDS             7130..7564
                     /gene="agaF"
                     /locus_tag="ACHKYO_RS20135"
                     /old_locus_tag="pgaptmp_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HGU4430789.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7561..8694
                     /gene="nagA"
                     /locus_tag="ACHKYO_RS20140"
                     /old_locus_tag="pgaptmp_004028"
     CDS             7561..8694
                     /gene="nagA"
                     /locus_tag="ACHKYO_RS20140"
                     /old_locus_tag="pgaptmp_004028"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU4430790.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYINTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNSIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            9045..10199
                     /locus_tag="ACHKYO_RS20145"
                     /old_locus_tag="pgaptmp_004029"
     CDS             9045..10199
                     /locus_tag="ACHKYO_RS20145"
                     /old_locus_tag="pgaptmp_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HGU4430791.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10212..11072
                     /gene="kbaY"
                     /locus_tag="ACHKYO_RS20150"
                     /old_locus_tag="pgaptmp_004030"
     CDS             10212..11072
                     /gene="kbaY"
                     /locus_tag="ACHKYO_RS20150"
                     /old_locus_tag="pgaptmp_004030"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HGU4430792.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11239..11715
                     /gene="agaB"
                     /locus_tag="ACHKYO_RS20155"
                     /old_locus_tag="pgaptmp_004031"
     CDS             11239..11715
                     /gene="agaB"
                     /locus_tag="ACHKYO_RS20155"
                     /old_locus_tag="pgaptmp_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HGU4430793.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11754..12557
                     /gene="agaC"
                     /locus_tag="ACHKYO_RS20160"
                     /old_locus_tag="pgaptmp_004032"
     CDS             11754..12557
                     /gene="agaC"
                     /locus_tag="ACHKYO_RS20160"
                     /old_locus_tag="pgaptmp_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HGU4430794.1"
                     /translation="MHEITLLQGLSLAALVFILGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12547..13338
                     /gene="agaD"
                     /locus_tag="ACHKYO_RS20165"
                     /old_locus_tag="pgaptmp_004033"
     CDS             12547..13338
                     /gene="agaD"
                     /locus_tag="ACHKYO_RS20165"
                     /old_locus_tag="pgaptmp_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HGU4430795.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13339..14094
                     /locus_tag="ACHKYO_RS20170"
                     /old_locus_tag="pgaptmp_004034"
     CDS             13339..14094
                     /locus_tag="ACHKYO_RS20170"
                     /old_locus_tag="pgaptmp_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HGU4430796.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         3499 a         3622 c         3721 g         3507 t
ORIGIN      
        1 accaggtcct cattttaata acccctggct ggagaatata gtacaatggc caacatcgaa
       61 atcagacaag aaacgccaac tgcgttttat ataaaagttc acgacacaga taatgtggca
      121 attattgtta atgataatgg cctgaaagca ggaacgcgtt ttccggatgg actggaatta
      181 attgaacata ttccccaggg gcataaagtc gcattgctgg acattccggc taatggtgaa
      241 attattcgtt atggtgaagt gattggttac gccgtgcgtg caatcccacg cggaagctgg
      301 atcgacgaat caatggttgt actaccggaa gcgccgccgt tacacacgct gccactggca
      361 accaaagtcc cggccccctt accgccgctg gaaggataca cctttgaggg ctatcgcaat
      421 gccgatggca gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg
      481 gcaggcgtgg tggactatgt agtaaaaatc attgaacgcg atctgctacc gaaatacccg
      541 aacgtcgatg gcgtggtggg gctgaatcat ttgtacggct gtggcgtggc gattaacgca
      601 ccggcggcag ttgtgcctat tcgtaccatc cacaatattt cgctgaaccc taactttggc
      661 ggcgaagtaa tggtgattgg tctgggttgt gaaaagttgc agcctgagcg cctgctggtc
      721 ggcacggatg atgtgcaagc tattccagta gaaagcgcca gcattgtcag tttgcaggat
      781 gaaaagcatg tcggttttca gtccatggtc gaggatattt tgcaggtcgc cgaacgccat
      841 ctacaaaaac tgaatcaacg tcagcgagaa acctgtccgg cttcagaact ggttgtcggc
      901 atgcagtgcg gtggcagcga tgccttttct ggcgtaacgg caaacccggc ggttggctat
      961 gcgtctgatc tactggtgcg ctgcggcgca acggtgatgt tctcagaagt cacggaagtg
     1021 cgtgacgcga tccatctgct gacaccacgc gcagtgaacg aagaggtcgg caaacggctg
     1081 ctggaggaga tggagtggta cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc
     1141 aacccttcgc cgggcaacaa gaaaggcggt ctggcaaacg tggtagagaa ggcactcggc
     1201 tccattgcta aatcgggtaa aagctcaatt gttgaagtgc tgtcgcccgg tcaacgcccg
     1261 actaaacgcg gattaattta cgccgcgacg ccagccagcg attttgtctg tggcacgcaa
     1321 caggtggctt cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc
     1381 ctgatggcgg tacccgtcat taaaatggcg acccgcaccg agctggcgaa ccgctggttt
     1441 gatttaatgg atattaacgc gggcactatc gccaccggcg aagaaaccat tgaagaggtg
     1501 ggctggaagt tgttccactt tattctcgac gtcgccagcg ggaagaagaa aaccttctcg
     1561 gatcaatggg ggctgcataa ccagctggca gtgtttaacc cggcaccggt gacctgattt
     1621 cttttggaac gtccctcgca aaacatggcc ttagtgccat gtttttattg tttaaagccc
     1681 ccacgtccat taataatgca tttgcattac cttagttcaa gcttataatt tgagcagaaa
     1741 acaggctgta aaaggacagt gaatcatgcc cgctaatgct cgctctcacg ctgtactgac
     1801 cactgaatca aaggtcacga tacgcggaca aacaactatc cccgcgccag tgcgtgaggc
     1861 cttaaaactg aagccaggcc aggacagcat tcattacgaa attctgcctg gtgggcaagt
     1921 ctttatgtgc cgactgggag atgaacagga ggatcatact atgaatgcat ttttgcgttt
     1981 tctggatgca gatatccaga acaacccgca aaaaactcgt ccattcaaca ttcaacaagg
     2041 aaagaaactt gtcgctggca tggacgtcaa cattgatgat gagattggcg acgacgaata
     2101 atggattttc cacaaagggt taatggttgg gcgctatatg ctcatccctg ttttcaggaa
     2161 acctacgacg ctttagttgc cgaagtcgag acattaaagg gaaaagatcc tgaaaattat
     2221 cagagaaaag ccgccacaaa gttattggcg gtagtccata aagtgattga ggagcatatc
     2281 acggtcaatc catcatcacc ggcattccgt catggcaagt cgttaggctc tgggaaaaat
     2341 aaagactggt cacgggtaaa atttggtgct ggtcgttatc gtctcttctt tcgttatagt
     2401 gaaaaagaga aagtcatcat tctgggatgg atgaacgatg aaaacactct gcgcacctac
     2461 ggtaaaaaaa cagatgccta taccgtattc agcaaaatgt taaaaagagg acatcctcct
     2521 gccgactggg aaagcctcac ccaagagaca gaagaaaacc attgatggtg ttcacaatgc
     2581 ccgccgaacg tattcgtgtt gaccagcggg caccctttct cactccccga ccagaatcac
     2641 ttcaacccca gcctttcgca gtccttccag actatccgca ggaatgcctt catcaacaat
     2701 gatcatgtcg atacgttgag tatcaatgat cttatgtaaa ctggaacgat tgaatttact
     2761 ggaatcggtg accacgatga tccgttccgc aacttcgcac atccgacggt ttaaacgggc
     2821 ttcatcttca ttatgtgtgc tgacgccgcg ctccagatcg atcgcatcta caccaagaaa
     2881 cagcatatcg aagtggtaat tttgcagcga ttgctcagcc tgatcgccgt aaaaagattg
     2941 cgactgacgg cgcaaatgcc cgccggtcat cagcagctca acgccttccg cttccagcaa
     3001 cgcattagcc acgttcatac cgttggtcat cgcaattacg tcagtgtgct tacgcatcag
     3061 acgagcaatc tcaaaagtgg tagtcccgga atcgaggatc acccgatgac caggctgaat
     3121 caactcaacg gcagctttcg caacgctgcg tttcatcgcg gtgttcagcg cgcttttatc
     3181 ttccactgat ggctcgactg acggcgtcgt gctatcgcag atcaacgcgc caccataggc
     3241 acgcacagcg atcccctgct tttccagaaa cgccagatca ttgcggatcg tcacagtaga
     3301 tacgccatac aatgccgaca gatcgttaac ctgcacactc ccttgctgtc gcagacgctg
     3361 aatgatctgt tctcgtcgct cgctggtgcc agtcactcgc ttctcacctg aagcgtcggt
     3421 attactcata gtaagtcctt tcgtaaaact ttcgtttcat ttcgttttgc ctattaacgc
     3481 ctttctatta agcaaatgca agcccacctt gcccattgac gcaagctact ctcgtttcag
     3541 tgactttcat tatgtttctt ttgtgaatca gatcagaaaa ctattatctt tcgttttatt
     3601 tttatctcac catgacgcag tatcaactga aacaaaacga aagattaata tcgcagtaat
     3661 ctgaactgga gaggaaagtg aaacatctga cagaaatggt gagacagcac aaagcgggca
     3721 aaacaaatgg aatttatgcc gtttgttccg cacatccgct ggtgctggag gctgcaatcc
     3781 gctacgccag tgcaaaccaa acgccgttac tgattgaagc aacctccaat caggtagacc
     3841 agttcggcgg ttataccgga atgacgcccg ccgattttcg cggctttgtt tgtcagctcg
     3901 ccgactcgtt gaatttcccg caggatgcgt tgattctggg tggtgaccat ctggggccaa
     3961 accgctggca aaacctgccg gccgctcagg caatggccaa tgccgatgat ttgattaaaa
     4021 gctacgttgc ggcaggattc aaaaaaatcc accttgattg cagcatgtcc tgtcaggacg
     4081 atccgattcc cttaactgat gacatcgtgg ctgaacgcgc cgcccgtctg gcgaaagtgg
     4141 cggaagaaac ctgtcttgaa cactttggcg aagccgatct ggagtatgtc attggtaccg
     4201 aagtgccggt acctggcggc gcgcatgaaa ccttaagcga gctggcggtc accacgccgg
     4261 atgccgcccg cgccacgctg gaagcccatc gtcacgcctt tgaaaagcaa ggtttgaatg
     4321 ccatctggcc acgcatcatt gccctggtgg ttcaacccgg cgtcgaattc gatcacacca
     4381 acgttattga ttatcagccc gccaaagcga gcgccttaag ccagatggtc gaaaactacg
     4441 aaacgctgat tttcgaagcg cactctaccg attatcaaac gccgcaatcg ctgcgccagc
     4501 tggtgattga ccactttgcc attctgaaag ttggcccagc gctgaccttc gccctgcgtg
     4561 aagctctgtt ctctctggcg gcgattgaag aagaactggt gccagcgaaa gcctgttctg
     4621 gtctgcgtca ggtgctggaa gacgtgatgc tcgaccgccc ggaatactgg caaagccact
     4681 accacggtga cggcaacgcg cgtcgtctgg cgcgtggtta tagctactcg gatcgcgtgc
     4741 gctattactg gccggacagc cagattgatg acgctttcgc tcatctggtt cgtaatctgg
     4801 cggattcacc aattccgcta ccgctcatca gccagtatct gccgctgcaa tacgtgaaag
     4861 ttcgctccgg cgagctgcaa ccgacgccac gggaactcat tatcaaccat attcaggaca
     4921 tcctggcgca gtactacaca gcctgtgaag gccaataagc aaaacaaaga ggaacacgct
     4981 atgccaaata ttgttttaag ccggattgat gaacgcttga ttcacggtca ggtcggcgtt
     5041 cagtgggtcg gatttgcggg ggcaaatctg gtgctggtag ccaacgatga ggttgccgaa
     5101 gatccggtac aacaaaacct gatggaaatg gtactggcag aagggatcgc cgtacgtttc
     5161 tggacgctgc aaaaagttat cgacaacatt catcgcgccg ccgatcgaca gaaaatcctg
     5221 ctggtttgta aaacacccgc cgatttcctg acgctggtga aaggtggcgt tccggtgaat
     5281 cgcattaacg ttggcaatat gcactacgcc aatggcaaac aacaaatcgc caaaacggtt
     5341 tctgtggatg cgggcgatat cgcagcattt aacgacctga aagccgctgg ggtggaatgc
     5401 ttcgttcagg gcgtcccgac agagcctgct gtggacctct ttaaattact ttgagggatt
     5461 catcatggaa atcagcctgt tgcaggcatt tgcgttgggc attatcgcct ttatcgctgg
     5521 cctggatatg tttaacggcc taacacatat gcaccgcccg gtggtcctcg gcccgttggt
     5581 cgggctggta cttggcgatc tgcataccgg aattttaacc ggcggtacgc tggaactggt
     5641 gtggatgggg ctggccccgc tggcgggcgc acagccgcct aacgtgatta tcggtactat
     5701 cgtcggcacg gcgtttgcca ttactactgg cgtgaaaccc gatgtcgcgg tcggtgtcgc
     5761 cgtacctttc gctgtcgcag tacagatggg gattaccttc ctgttctcgg tgatgtctgg
     5821 cgtgatgtct cgctgcgacc ggatggcgga gaacgccgat actcgcggca ttgaacgcgt
     5881 gaactatctg gcactgctgg cactcggcac tttctatttt ctctgcgctt tcctgcctat
     5941 ctacttcggc gcggaacatg caaaaaccat cattgatgtc ctgccgcaac gattaatcga
     6001 cggcctcggc gtcgcgggcg gcatcatgcc agcaatcggt tttgccgtgc tgctgaaaat
     6061 catgatgaaa aacgtctaca tcccctactt catcctgggc tttgttgccg cagcctggct
     6121 caagttaccg gtgctggcta ttgctgccgc tgcgctggcg atggcgctga tcgacctgct
     6181 gcgtaaatct cctgaaccga ctcaacctgc ggcacagaaa gaggaatttg aagatggcat
     6241 ctaatcaaac caccctgccg aacgtcagtg aaaacgaaga aacattgctg actggcgtca
     6301 atgaaaacgt gtatgaagat cagagcattg gcgcggagct gacgaaaaaa gatatcaacc
     6361 gcgtcgcctg gcgttccatg ctgttacaag cgtcttttaa ctacgaacgt atgcaggctt
     6421 ctggctggtt gtacggtctg ctgcctgctc tgaaaaagat ccacactaat aaacgcgacc
     6481 tggcgcgcgc catgaagggg catatgggtt tcttcaatac ccatccgttt ctggtgacat
     6541 ttgttatcgg cattatcctt gcgatggagc gttctaagca ggacgttaac agtattcaga
     6601 gcaccaaaat tgccgtcggt gcgccgctcg gcgggattgg tgatgcgatg ttctggctga
     6661 cgctactgcc gatttgtggc gggataggcg caagccttgc gctacaaggc tctattcttg
     6721 gcgcagtcgt atttattgtg ctgttcaacg tggtacacct ggggctgcgt tttggtctgg
     6781 cgcattatgc ttaccgcatg ggcgtggcgg cgattccact cattaaagcg aataccaaaa
     6841 aagtcggcca tgctgcgtct atcgttggga tgacggtaat cggcgcgctg gtggcaacct
     6901 atgttcgttt aagcaccacg ctggaaatca ctgcgggcga cgcagtggtt aagttacaga
     6961 ctgatgttat tgacaaactg atgcctgcct tcttaccgct ggtctacacc ttgaccatgt
     7021 tctggctggt acgccgcggc tggagtccgc tgcgtctgat tgcagttacc gtggttctcg
     7081 gcatcgtcgg taaattctgc catttccttt aaatacaatg aggtattaga tgttaagtat
     7141 tattttgaca gggcatggcg ggtttgccag cggcatggaa aaagcaatga agcaaattct
     7201 cggtgagcaa tcgcagttta tcgccatcga ttttccggaa acctcaagca ccgcgctgct
     7261 tacatcgcag cttgaagagg ccatcgccca actggattgt gaagacggca ttgtttttct
     7321 gacggatttg ctgggcggca cgccgtttcg cgtagcttca acgctggcga tgcaaaaacc
     7381 gggctgtgaa gtgatcaccg gcaccaattt gcaactgcta ctggagatgg tgctggagcg
     7441 cgaagggtta agcggtgaag aattccgtgt acaggcgctg gagtgtggac accgagggct
     7501 gaccagtctg gtggacgagt taggccgctg ccatgaagaa tgtccggtcg aggaaggaat
     7561 atgacacacg ttctgcgcgc cagaaggctg ctgactgaag agggatggct cgatgaccat
     7621 cagttgcgta ttgctgacgg tgtcatcgca gcaatcgaac cgattccagt gggcgtgact
     7681 gaacgcgatg cggaactgct ctgccccgct tacatcaaca cccatgtaca cggtggtgcg
     7741 ggcgttgatg taatggatga cgcgccggat gtactcgaca agctggcaat gcacaaggca
     7801 cgcgaaggtg tcggcagttg gttgccgact accgtaaccg cgccgcttaa cagcattcat
     7861 gcggcgctga aacgtattgc tcaacgttgc caacgcggcg gacctggtgc gcaagtgctg
     7921 gggagttatc tcgaaggacc gtacttcacg ccgcagaata aaggcgcgca tccgccggaa
     7981 ttgtttcgcg agcttgaaat tgccgagctg gatcaattga ttgccgtttc tcagcacacc
     8041 ttacgcgtgg tagcgctggc accggaaaaa gagggggcat tgcaggccat ccgccatctt
     8101 aaacagcaaa atgtacgagt gatgctgggg catagcgcgg cgacctggca acaaactcgc
     8161 gccgcgtttg atgctggtgc cgacggcctg gtgcattgct ataacgggat gacaggttta
     8221 catcaccgcg aaccgggaat ggttggcgcg ggattaacgg acaagcgcgc ctggctggaa
     8281 ctgatagccg atggtcatca tgtgcatccg gcggcgatgt cgctgtgttg ttgctgtgca
     8341 aaagagagaa tcgtgctgat caccgacgcg atgcaggcag ccgggatgcc ggatggtcgc
     8401 tatacgttat gtggcgaaga agtgcagatg cacggtggcg ttgtccgtac cgcgtccggt
     8461 gggctggcgg gcagtacgct gtctgttgat gcggcagtgc gcaacatggt cgagttgacg
     8521 ggcgtaacgc ctgcggaagc cattcatatg gcatcgctgc atccggcgcg aatgctgggt
     8581 gttgatggtg ttctgggatc gcttaaaccg ggcaaacgcg ccagcatcgt tgcgctggat
     8641 agcgggctgc atgtgcaaca aatctggatt cagagtcaat tagcttcgtt ttgatatttt
     8701 gctcctttat tgggccttca cttcccccgt aaggcctttc tttttctttc gttttgatct
     8761 gtgcagcggt gtcggatgcg acgctaacgc gtcttatgcg acctacggta cgccgcatcc
     8821 agcaatcggt gaacatagcg acgtcattac gcaattcttt aatcttccct tgtcttttct
     8881 ttcaatgatc acagatttcg ttttatttcc tttttctcca ttgaactttc ggtttctttt
     8941 ctatagattt taatcaacga aagacatcac caagtgaaat gaaacgaaag gcaagtgaaa
     9001 gcgacaacgc ccgacgtcaa gttcatcaga ctaaggattg agttatgcca gaaaattaca
     9061 cccctgctgc cgccgcaacc ggtacatgga ctgaagaaga gatccgccat cagcctcgcg
     9121 catggatccg ttcactcacc aacatcgacg cgctacgttc tgcgctcaat aacttccttg
     9181 aaccgttact gcgcaaagag aatctgcgga tcatcctgac cggagccgga acctcggcat
     9241 ttatcggtga catcatcgcg ccgtggctcg ccagccatac cggtaaaaac ttcagcgccg
     9301 taccgaccac cgatctggtc accaatccga tggactacct gaacccagct catccgctgc
     9361 tgttgatctc cttcggtcga tccggcaaca gcccggaaag cgtcgctgcc gtggaactgg
     9421 caaatcaatt tgtaccagaa tgctatcacc tgccgatcac ctgcaacgaa gcgggcgctc
     9481 tttaccaaaa cgcgatcaac agcgataacg cgtttgccct gctgatgccc gcagaaacgc
     9541 acgatcgcgg ctttgcgatg accagcagca ttaccaccat gatggccagc tgcctcgcgg
     9601 ttttcgcacc tgagacgatc aacagccaaa ccttccgcga cgtggcggat cgttgccagg
     9661 cgatcctgac ctcactgggc gatttcagcg aaggtgtgtt tggttacgca ccgtggaaac
     9721 ggatcgttta tctcggcagc ggtggcttac agggcgcagc acgcgagtcg gcgctgaaag
     9781 tgctggaact gactgcgggt aaactggcgg ccttctatga ttccccgacc ggattccgtc
     9841 atggcccgaa atcactggtc gataacgaaa cgctggtggt ggtatttgtc tcaagccacc
     9901 cttacacccg tcagtatgat cttgatctgc tggctgaact ccgccgtgac aaccaggcaa
     9961 tgcgcgtaat cgccatcgcc gcggaaagca ccgacatcgt cgctgccggt ccacatatta
    10021 tcctgccgcc gtcacgtcac tttatcgacg ttgagcaggc attttgcttc ctgatgtacg
    10081 cccagacgtt tgcactgatg cagtcgctgc acatgggcaa tacgccggat accccatcag
    10141 ccagcggcac cgttaaccgc gtggtgcaag gcgtaatcat tcatccgtgg caggcataag
    10201 aggatcgcat tatgagcatt atctccacta aatatctgtt acaggacgcc caggccaatg
    10261 gctacgcggt gcctgctttt aacattcata acgccgagac gatccaggcg atcctcgaag
    10321 tgtgcagtga aatgcgatcg ccggtgatcc tcgccggaac gccggggacc tttaaacata
    10381 tcgcgctgga agagatctac gccctgtgca gcgcctattc cacaacctac aacatgccac
    10441 tggcgctgca tctcgaccac cacgaatcgc tggatgatat tcgccgtaaa gtccacgcag
    10501 gtgtgcgcag cgcaatgatc gacggcagcc acttcccgtt tgccgagaac gtgaagctgg
    10561 tgaaatcggt tgttgacttc tgccactcac aggattgcag cgtggaagca gaactgggcc
    10621 gcctgggcgg tgttgaagat gacatgagcg ttgacgccga aagtgcattc ctgaccgatc
    10681 cacaagaagc taaacgcttt gtcgaactga ctggcgtcga cagcctggcg gtagcgattg
    10741 gtacggcgca cggcttatac agcaaaacgc cgaagattga tttccagcgg ctggcggaaa
    10801 ttcgtgaagt ggtggatgtt cctctggtgc tacatggtgc cagcgatgtt ccggatgaat
    10861 ttgtccgtcg cactattgaa cttggcgtca caaaagtgaa cgttgccaca gaattaaaaa
    10921 tagccttcgc tggcgcggtt aaagcctggt ttgcggaaaa tccgcagggt aatgatcctc
    10981 gttattatat gcgcgtcgga atggatgcga tgaaagaagt tgtcagaaat aaaattaatg
    11041 tctgtggttc agcgaatcga atttcagcat aatcattagt tttttatgat ttatcccaat
    11101 gtacttcccg attaatatcg gggagtgcct taatggaaaa ggagatgact aaaccttaat
    11161 aaatacatca gtacaatatc gcaacaataa tatattaaaa aaaattatat tattcaactt
    11221 tatggtgagg attacacaat gagcagtcca aatattctct taacccgtat tgataaccgt
    11281 ctggttcacg gtcaggttgg cgtgacctgg acatccacca tcggtgcgaa tctgctggta
    11341 gtcgtggatg atgttgtcgc taacgatgat attcaacaga aattaatggg tattaccgca
    11401 gaaacctacg gctttggcat tcgtttcttt actatcgaaa aaaccattaa cgtcatcggc
    11461 aaagctgcac cgcatcagaa gatcttcctg atttgccgta cgccacaaac ggtacgtaaa
    11521 ttggtagaag gtggcattga cctgaaagat atcaacgtcg gcaatatgca tttctcggaa
    11581 gggaaaaagc aaatcagcag taaagtttat gtcgatgacc aggatctcgc ggacttacgt
    11641 tttattaaac aacgtggcgt gaatgttttc attcaggacg tccctggcga tcaaaaagaa
    11701 caaatccctg actaaatcta aaatcgcctt aatattggtt tgaggtaata aaaatgcatg
    11761 aaataaccct acttcaggga ttatccctag cggcgttagt tttcattctg gggattgatt
    11821 tttggctgga agccttattt ttattccgcc cgataatcgt ttgtacccta actggcgcta
    11881 ttctcggtga tattcagact ggcttaatta ccggtggtct gacagagttg gctttcgccg
    11941 gattaacccc tgcaggtggt gttcagccgc ccaacccgat tatggcgggt ctgatgacca
    12001 ccgtcattgc atggtctacg ggcgttgatg ccaaaacagc aattggtctt ggcctgccgt
    12061 ttagtttgtt aatgcagtac gtcattctgt tcttctattc cgctttctca ttatttatga
    12121 ccaaagccga taaatgcgcg aaagaggcgg atacggcagc gttttcccgg cttaactgga
    12181 caacgatgct catcgtcgct tcagcgtatg cggtgattgc tttcctctgt acttacctgg
    12241 cacaaggggc gatgcaggcg ctggtgaaag cgatgcccgc ctggttgacc cacggctttg
    12301 aagtggctgg cggtattctg cctgccgttg gttttggctt gctgctgcgc gtaatgttca
    12361 aagcgcaata tatcccttac ctgatcgccg gtttcctgtt tgtttgctac atccaggtca
    12421 gcaacctgtt gccggttgcc gtactgggcg caggctttgc ggtgtatgag tttttcaatg
    12481 cgaaatcccg gcagcaagcg caaccgcagc ccgttgccag taaaaatgaa gaagaggact
    12541 acagcaatgg gatctgaaat cagtaaaaaa gatatcaccc gtctgggctt tcgttcgtcg
    12601 ctgctgcaag cgagctttaa ctacgaaagg atgcaggcgg gcggttttac ctgggcgatg
    12661 ttgccgatcc tgaaaaagat ttataaggac gacaaaccgg gcttaagcgc ggcgatgaaa
    12721 gataacctcg aatttattaa tacccacccg aatctggtcg gattcctgat ggggttatta
    12781 atttcgatgg aagaaaaagg agaaaaccgc gacaccatta aaggcctcaa agtggcactg
    12841 tttggcccaa tcgccgggat tggcgatgcg attttctggt ttactttgtt gccgattatg
    12901 gcgggaattt gctcatcatt tgccagccag ggaaacctgc tggggccgat tctatttttc
    12961 gccgtttacc tgcttatctt tttcctgcgc gtcggctgga cccacgtcgg ttattcagtc
    13021 ggcgtgaagg cgatcgataa agtgcgagag aactcgcaga tgattgctcg ctcggcaacc
    13081 atcctcggaa tcacggtaat cggcgggctg atcgcttcgt atgtgcatat taacgtggtg
    13141 acatcgtttg ccatcgacag tacccacagc gtcgcgctgc aacaggattt cttcgataaa
    13201 gtcttcccga acattttacc gatggcctac accctgctga tgtattactt cctgcgggtg
    13261 aaaaaagcgc atccggtgct gttaatcggc gtgacttttg tgctctctat tgtttgttcc
    13321 gcattcggca ttttgtaaat ggaacgaggc actgcttctg gcggtgcctc tttacttaag
    13381 gaatttcatc ctgtgcaaac ccttcagcaa gttgaaaact atacggcgtt aagtgaacga
    13441 gccagcgaat atttattggc tgtgatccgt agcaaaccgg atgccgtgat ttgcctggcg
    13501 accggagcca cgccattact gacgtatcat tatctggtag aaaaaatcca ccagcagcag
    13561 gttgatatca gccagctcac cttcgtgaag ctcgacgaat gggtggatct gccattaacg
    13621 atgcctggca cctgcgaaac tttcctacaa cagcatatcg tgcagccgct ggggctgcgt
    13681 gaagaccagc tcatcagctt tcgctccgaa gagataaatg agacagagtg cgaacgggta
    13741 acgaacctga ttgcgcgcaa aggcggtctg gatttatgcg ttctaggatt ggggaaaaac
    13801 ggtcatcttg ggctgaacga accgggagaa agcctacaac cggcctgcca tatcagtcaa
    13861 cttgatgcca gaacacagca acatgagatg ttaaaaacca cgggtcgccc cgtgactcgt
    13921 gggatcacct taggcctgaa ggatattctc aatgcccgcg aggttttgtt attggtgact
    13981 ggcgaaggaa agcaggatgc gacagaacgt tttctcacgg ctaaagtctc caccgctatc
    14041 ccggcttcgt ttttatggct gcacagtaac tttatttgtt taattaatac ctaaccagat
    14101 gaaaatttgc tcaaaatttt taaattaact tattcaacag tcacgcatta tattaaataa
    14161 catttgactg ggttgaacat aacgccaata gcaaaaggag ttatgttcaa caatttaatt
    14221 ttagatatac atataacttg ttctattaat aaatcacctc gtattctgac tcggcccctg
    14281 gcgcagcaaa gtcaggcatt tatactcttt ttaacacata gaaaattcat atcaatataa
    14341 taaaaacat
//
LOCUS       DAZGAN020000121        14204 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGAN020000121 DAZGAN020000000
VERSION     DAZGAN020000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.121"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            281..580
                     /gene="yoaC"
                     /locus_tag="ACHKYO_RS20175"
                     /old_locus_tag="pgaptmp_004035"
     CDS             281..580
                     /gene="yoaC"
                     /locus_tag="ACHKYO_RS20175"
                     /old_locus_tag="pgaptmp_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HGU4430797.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERTVIKNPEYFSTYMQEELKALV"
     gene            complement(700..879)
                     /gene="yoaH"
                     /locus_tag="ACHKYO_RS20180"
                     /old_locus_tag="pgaptmp_004036"
     CDS             complement(700..879)
                     /gene="yoaH"
                     /locus_tag="ACHKYO_RS20180"
                     /old_locus_tag="pgaptmp_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HGU4430798.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            953..2314
                     /gene="pabB"
                     /locus_tag="ACHKYO_RS20185"
                     /old_locus_tag="pgaptmp_004037"
     CDS             953..2314
                     /gene="pabB"
                     /locus_tag="ACHKYO_RS20185"
                     /old_locus_tag="pgaptmp_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HGU4430799.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKHTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2318..2896
                     /gene="nudL"
                     /locus_tag="ACHKYO_RS20190"
                     /old_locus_tag="pgaptmp_004038"
     CDS             2318..2896
                     /gene="nudL"
                     /locus_tag="ACHKYO_RS20190"
                     /old_locus_tag="pgaptmp_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HGU4430800.1"
                     /translation="MEYRSLTLDYFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3080..4444
                     /gene="sdaA"
                     /locus_tag="ACHKYO_RS20195"
                     /old_locus_tag="pgaptmp_004039"
     CDS             3080..4444
                     /gene="sdaA"
                     /locus_tag="ACHKYO_RS20195"
                     /old_locus_tag="pgaptmp_004039"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU4430801.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMCFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4575..6173
                     /gene="pdeD"
                     /locus_tag="ACHKYO_RS20200"
                     /old_locus_tag="pgaptmp_004040"
     CDS             4575..6173
                     /gene="pdeD"
                     /locus_tag="ACHKYO_RS20200"
                     /old_locus_tag="pgaptmp_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4430802.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6177..7733)
                     /gene="yoaE"
                     /locus_tag="ACHKYO_RS20205"
                     /old_locus_tag="pgaptmp_004041"
     CDS             complement(6177..7733)
                     /gene="yoaE"
                     /locus_tag="ACHKYO_RS20205"
                     /old_locus_tag="pgaptmp_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HGU4430803.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7721..7879)
                     /gene="yoaL"
                     /locus_tag="ACHKYO_RS20210"
                     /old_locus_tag="pgaptmp_004042"
     CDS             complement(7721..7879)
                     /gene="yoaL"
                     /locus_tag="ACHKYO_RS20210"
                     /old_locus_tag="pgaptmp_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HGU4430804.1"
                     /translation="MDRHRRHFSIRPFNACLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8196..9167
                     /gene="manX"
                     /locus_tag="ACHKYO_RS20215"
                     /old_locus_tag="pgaptmp_004043"
     CDS             8196..9167
                     /gene="manX"
                     /locus_tag="ACHKYO_RS20215"
                     /old_locus_tag="pgaptmp_004043"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HGU4430805.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9230..10030
                     /gene="manY"
                     /locus_tag="ACHKYO_RS20220"
                     /old_locus_tag="pgaptmp_004044"
     CDS             9230..10030
                     /gene="manY"
                     /locus_tag="ACHKYO_RS20220"
                     /old_locus_tag="pgaptmp_004044"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HGU4430806.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10043..10894
                     /gene="manZ"
                     /locus_tag="ACHKYO_RS20225"
                     /old_locus_tag="pgaptmp_004045"
     CDS             10043..10894
                     /gene="manZ"
                     /locus_tag="ACHKYO_RS20225"
                     /old_locus_tag="pgaptmp_004045"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HGU4430807.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10949..11407
                     /gene="yobD"
                     /locus_tag="ACHKYO_RS20230"
                     /old_locus_tag="pgaptmp_004046"
     CDS             10949..11407
                     /gene="yobD"
                     /locus_tag="ACHKYO_RS20230"
                     /old_locus_tag="pgaptmp_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HGU4430808.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            11836..12402
                     /gene="mntP"
                     /locus_tag="ACHKYO_RS20235"
                     /old_locus_tag="pgaptmp_004047"
     CDS             11836..12402
                     /gene="mntP"
                     /locus_tag="ACHKYO_RS20235"
                     /old_locus_tag="pgaptmp_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HGU4430809.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(12399..13208)
                     /gene="rlmA"
                     /locus_tag="ACHKYO_RS20240"
                     /old_locus_tag="pgaptmp_004048"
     CDS             complement(12399..13208)
                     /gene="rlmA"
                     /locus_tag="ACHKYO_RS20240"
                     /old_locus_tag="pgaptmp_004048"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HGU4430810.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(13374..13583)
                     /gene="cspE"
                     /locus_tag="ACHKYO_RS20245"
                     /old_locus_tag="pgaptmp_004049"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(13374..13583)
                     /gene="cspE"
                     /locus_tag="ACHKYO_RS20245"
                     /old_locus_tag="pgaptmp_004049"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU4430811.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(13596..13739)
                     /gene="yobF"
                     /locus_tag="ACHKYO_RS20250"
                     /old_locus_tag="pgaptmp_004050"
     CDS             complement(13596..13739)
                     /gene="yobF"
                     /locus_tag="ACHKYO_RS20250"
                     /old_locus_tag="pgaptmp_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HGU4430812.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT         3545 a         3529 c         3504 g         3626 t
ORIGIN      
        1 aaaactaact atataaataa ccataaccat gccgttgtgt tcgccctctg actagcaact
       61 ctggttttct ttcttagctg aataatctgg ttatagcgtg aggactgacc gttaaatttc
      121 tccatcttac tggataacac accatgagct gctaccttta aaacacctga atgtcaacag
      181 gttaactcgt gccatatcgt tataatccct ttgatattag atgcaaatta aggtcatata
      241 tagtcttatc gataacatgg ttaattttaa ggataagaat atgcctgctg taatagataa
      301 agccctggat ttcattggtg ccatggatgt atcagcgcca acaccaagtt cgatgaatga
      361 aagcacagcg aagggaatct ttaaatattt aaaagaactg ggagtacccg cgagtgccgc
      421 tgatattacg gcgcgagctg accaggaagg ctggaaccct gggttcacgg aaaaaatggt
      481 tggatgggca aaaaaaatgg agtctggtga acgcactgta attaaaaatc ctgaatactt
      541 ttcaacatat atgcaggaag aactaaaagc actggtctga gcttaattta tatcagcata
      601 aatgggtcag gacgctttta atcacatata aaaagcgtcc tttttctcac aatcaaataa
      661 tcatcggatt ctataacgaa atcgatacgg attagtgtgt tactcgtctt catcctcaaa
      721 acgcgccacg attcgctcgc cggagtggtt ggcacgcagc tcttctgcca ccagcgcgat
      781 tgcctggccg ctgctcatcc cttgtgccat cagttcctgg atccgctcga cagctttttg
      841 ctgctgttca tgggtgagtg aaggtaaacc tgcaaacatt gttaactcct gctaaattgt
      901 tggcgctaat tatttcatgc tacccggcac atagccagta gagtcaggac tgatgaagac
      961 gttatctccc gctgtgatta ctttaccctg gcgtcaggac gccgctgaat tttatttctc
     1021 ccgcttaagc cacctgccgt gggcgatgct tttacactcc ggctatgccg atcatccgta
     1081 tagccgcttt gatattgtgg tcgccgatcc gatttgcact ttaaccactt tcggtaaaga
     1141 aaccgttgtt agtgaaagcg aaaaacacac aacgaccact gatgacccgc tacaggtgct
     1201 ccagcaggtg ctggatcgcg cagacattcg cccaacgcat aacgaagatt tgccatttca
     1261 gggcggcgca ctggggttgt ttggctacga tctgggccgc cgttttgagt cactgccaga
     1321 aattgcggaa caagatatcg ttctgccgga tatggcagtg ggtatctacg attgggcgct
     1381 cattgtcgac caccagcgtc atacagtttc tttgctgagt cataatgatg tcaatgcccg
     1441 tcgggcctgg ctggaaagcc agcaattctc gccgcaggaa gatttcacgc tcacttccga
     1501 ctggcaatcc aatatgaccc gcgagcagta cggcgaaaaa tttcgccagg tacaggaata
     1561 tctgcacagc ggtgattgct atcaggtgaa tctcgcccag cgttttcatg cgacctattc
     1621 tggcgatgaa tggcaggcat tccttcagct taatcaggcc aaccgcgcgc catttagcgc
     1681 ttttttacgt cttgaacagg gtgcaatttt aagcctttcg ccagagcggt ttattctttg
     1741 tgataatagt gaaatccaga cccgcccgat taaaggcacg ctaccacgcc tgcccgatcc
     1801 tcaggaagat agcaaacaag cagaaaaact ggcgaactca gcgaaagatc gtgccgaaaa
     1861 tctgatgatt gtcgatttaa tgcgtaatga tatcggtcgt gttgccgtag ccggttcggt
     1921 aaaagtacca gagctgttcg tggtggaacc cttccctgcc gtgcatcatc tggtcagcac
     1981 tataacggcg cgactaccag aacagttaca cgccagcgat ctgctgcgcg cagcttttcc
     2041 tggtggctca ataaccgggg ctccgaaagt acgggctatg gaaattatcg acgaactgga
     2101 accgcagcga cgtaatgcct ggtgcggcag cattggctat ttgagctttt gcggcaacat
     2161 ggataccagc attactatcc gcacgctgac tgccattaac ggacaaatat actgctctgc
     2221 gggcggtgga attgtcgccg atagccagga agaagcggaa tatcaggaaa cttttgataa
     2281 agttaataag atattacgcc aactggagaa gtaagacgtg gaataccgta gcctgacgct
     2341 tgattatttt ttatctcgct ttcaactttt gcgcccgcaa attaaccggg aaaccctaaa
     2401 tcatcgtcag gctgccgtgt taatccccat cgtccgtcga ccgcaaccgg ggttgttgct
     2461 gactcagcgt tcaattcatc tgcgtaaaca cgctggacaa gtggcattcc ctggaggtgc
     2521 agtcgatgac acggacgcat cagttatcgc cgccgcgctg cgcgaagctg aagaagaggt
     2581 cgctataccg ccttccgccg ttgaagttat cggcgtgctg ccgcccgtcg atagcgtaac
     2641 tggctatcag gtaaccccag tggtcggcat tatcccgccc gatctgccgt atcgcgccag
     2701 tgaagatgaa gtctcggcgg tgtttgaaat gccgctcgcc caggcattac atctgggtcg
     2761 ttatcaccct ttagatatct accgccgtgg cgattcacat cgggtatggc tgtcctggta
     2821 cgaacagtat tttgtatggg gaatgaccgc aggcataatt cgtgagctgg cgctgcaaat
     2881 tggtgtgaaa ccctgactat acttatcttt acatctacaa aacactactt gagacaatca
     2941 tcgcaatatt agttaaatcg cggtttttga ttagtttaat tcatgtgaat agttaagcca
     3001 gtcgccgcgt tccctcttac actatgcgct gttattagtt cgttactgga agtccagtca
     3061 ccttgtcagg agtattatcg tgattagtct attcgacatg tttaaggtgg ggattggtcc
     3121 ctcatcttcc cataccgtag ggcctatgaa ggcgggtaaa cagttcgtcg atgatctggt
     3181 cgaaaaaggc ttactggata gcgttactcg cgttgccgtg gacgtttatg gttcactgtc
     3241 gctgacgggt aaaggccacc acaccgatat cgccattatt atgggtcttg caggtaacga
     3301 acctgccacc gtggatatcg acagtattcc cggttttatt cgcgacgtag aagagcgcga
     3361 acgtctgctg ctggcacagg gacggcatga agtggatttc ccgcgcgaca acgggatgtg
     3421 ttttcataac ggcaacctgc cgctgcatga aaacggtatg caaatccacg cctataacgg
     3481 cgatgaagtc gtctacagca aaacttatta ttccatcggc ggcggtttta tcgtcgatga
     3541 agaacacttt ggtcaggatg ctgccaacga agtgagcgtg ccgtatccgt tcaaatctgc
     3601 caccgaactg ctcgcgtact gtaatgaaac cggctattcg ctgtctggtc tcgctatgca
     3661 gaacgaactg gcgctgcaca gcaagaaaga gatcgacgag tatttcgcgc atgtctggca
     3721 aaccatgcag gcatgtatcg atcgcgggat gaacaccgaa ggtgtactgc caggcccgct
     3781 gcgcgtgcca cgtcgtgcgt ctgccctgcg ccggatgctg gtttccagcg ataaactgtc
     3841 taacgatccg atgaatgtca ttgactgggt aaacatgttt gcgctggcag ttaacgaaga
     3901 aaacgccgcc ggtggtcgtg tggtaactgc gccaaccaac ggtgcctgcg gtatcgttcc
     3961 ggcagtgctg gcttactatg accactttat tgaatcggtc agcccggaca tctatacccg
     4021 ttactttatg gcagcgggcg cgattggtgc attgtataaa atgaacgcct ctatttccgg
     4081 tgcggaagtt ggttgccagg gcgaagtggg tgttgcctgt tcaatggctg ctgcgggtct
     4141 tgcagaactg ctgggcggta gcccggaaca ggtttgcgtg gcggcggaaa ttggcatgga
     4201 acacaacctt ggtttaacct gcgacccggt tgcagggcag gttcaggtgc cgtgcattga
     4261 gcgtaatgcc attgcctctg tgaaggcgat taacgccgca cggatggctc tgcgccgcac
     4321 cagtgcaccg cgcgtctcgc tggataaggt catcgaaacg atgtacgaaa ccggtaagga
     4381 catgaacgcc aaataccgcg aaacctcacg cggtggtctg gcaatcaaag tccagtgtga
     4441 ctaatacttc ttactcgccc atctgcaacg gatgggcgaa tttatacccg ctttctcgtc
     4501 tgctgtaata ttccccacta cacttccact gttgcgtcag gcgtttgtcg ccatacgctt
     4561 acagggtggc ccgcatgcaa aaagcacaac ggatcattaa aacctatcgc cgtaatcgaa
     4621 tgattgtttg tacgatttgc gcactcgtta cgctcgcttc gaccctgagc gtgcgattta
     4681 tttcacagcg taacttaaat caacaacggg tagtacaatt cgccaatcac gctgtagagg
     4741 aattagataa agtactgctt cccctacagg caggtagcga agtcttgctt ccgctgattg
     4801 gtctgccctg ctctgtcgcc catttgccat tacgtaaaca ggcggcaaaa ctccaaactg
     4861 tgcgatccat tggcctggtg caagacggca cactttattg ctccagcatt tttggttatc
     4921 gcaatgtgcc cgtcgtggac attctggctg aacttcctgc accgcaacca cttttacgcc
     4981 tgacgatcga ccgtgccctg attaaaggca gtccggtttt gattcaatgg acgcctgcag
     5041 cgggcagtag caatgctggg gtcatggaga tgattaacat cgacttactg acggcaatgc
     5101 tccttgagcc acaactgccg caaatcagta gcgccagcct gacggtggac aaacggcatt
     5161 tgctctatgg taatgggctg gtagattccc ttccgcaacc tgaagacaat gaaaactatc
     5221 aggtttcttc gcaacgcttt ccttttacca ttaacgttaa tggtccgggg gctacggcgc
     5281 tggcatggca ctatcttcca acacaattac cgctggcggt gctgctaagt ttactggtgg
     5341 gctacatcgc ctggctggcg accgcttacc ggatgagctt ttcccgcgaa atcaatctgg
     5401 gcctggcgca acatgagttc gaattgttct gtcagccttt gcttaatgcg cgcagccagc
     5461 aatgtattgg tgtagagatt ttgctgcgct ggaacaatcc gcgtcagggc tggatttcac
     5521 cggatgtgtt tattcctatc gcggaagaac atcatttaat tgtgccactg acccgctatg
     5581 tgatggcaga aaccattcgt cagcgccatg ttttcccgat gagtagtcag tttcatgttg
     5641 gcattaacgt cgcacccagc cattttcgcc gtggtgtgct gataaaagat ctcaatcagt
     5701 actggtttag cgctcacccg attcagcaac tgatcctcga aatcaccgaa cgcgatgcct
     5761 tactggatgt tgattatcgg attgcccgcg agctacatcg taaaaacgtc aaactggcga
     5821 ttgatgactt cggcaccggc aacagttcat tttcctggct tgaaacatta cgtcctgacg
     5881 tgctgaaaat tgataagtca tttaccgcag ctataggttc tgacgcggtt aactcgacgg
     5941 tgaccgatat catcatcgcg ctggggcaaa gactgaatat tgaactggtg gcggagggcg
     6001 tggaaacgca agaacaggcg aagtatttgc gccgtcatgg cgtgcatatt ttgcaagggt
     6061 atttgtacgc acagccgatg ccgctacgtg attttcccaa atggctggcg ggcagccaac
     6121 cgccgcccgc ccggcataat ggacatatca cgcccattat gccgttacgt taacgcttac
     6181 tcatcttcat cgtgcgccgg ttgctcttta acaatgcgaa ccagatcaac acgataatca
     6241 ttggcttcaa tgatggtgat atgcagtggc cctacatcaa tcacatcgcc cacacgggga
     6301 atgtgaccat ttgccgagat caccaggccc gcgaccgtcg cgatatcatc gtcatcggca
     6361 aggtgctcaa catcaagcgc ctgctgcaag gcatgcaaat ctgtaccgcc ttttaccagc
     6421 cagccgtcac catcggtaat gatttccggc gtttcgtcag cgtccgggaa ttcacccgca
     6481 atggcttcca gcacatccag cggcgtgacc aagccttgta ccacaccaaa ctcgttggtc
     6541 acgataacaa agctcccgcg agcgcgacgc agcacgccca gcaggttgat cggatcgagg
     6601 gtttccggga cgataatcgc cggagacgcc gaagcaatcg ccgccacatc aacgccctct
     6661 tccagcgcca ccagcagttc tttagcacgt acgattccga tgatttcatc cagttcaccg
     6721 cgacataccg ggaacaggct gtgcggtgag gagagcagct gctcgcggat ttcatcgacc
     6781 ccgagattag cgtcaaccca gcttatttca ccgcgcggcg tcatgatccc gcgcagagaa
     6841 cgcgacgcca gcgtcagtac gccgttaatc atgtaacgtt cttcttcggc aaatgcacct
     6901 tccgggatcg gcatcggcat cgggttatcg gcatcgtgct gaacattggc ctgacgtttc
     6961 ccgcccatca aacgcaggat ggcatcggca gtacgcgctc gcagcggcaa agtcgactgg
     7021 tgacgaataa agttgcgacg cgcaatctgg ttaaacactt cgatgatgat cgagaagcca
     7081 atcgcggcat acaggtaacc tttcggaatg tggaaaccga aaccttctgc caccagactc
     7141 agaccaatca ttaacaggaa gctcagacag agcaccacca ccgtggggtg ctggttaacg
     7201 aatcgcgtca gcggtttgga tgccagcaac ataaccgcca tcgcaatcac taccgccgcc
     7261 atcatcaccg gcagatggtt aaccatccct actgcagtaa ttaccgcatc caacgagaag
     7321 acggcgtcaa ggatgacaat ctgtgtgacg accacccaga aactggcgta gcctttaccg
     7381 tggccggaat catgatcgcg gttttccagc cgttcatgca gttcggttgt tgctttgaac
     7441 agcaagaata tccccccgaa caacataatc aggtcgcgtc cggagaagga gaaatccatg
     7501 acggtaaata gcggtttggt cagcgtgacc atccatgaaa tcagcgacag cagccccaga
     7561 cgcataatca gcgccagtga taaccccagc aaacgcgctt tatcgcgttg ttttggcggc
     7621 agtttgtcag caagaatggc gatgaagacc aggttatcga tacccagcac aatttcgaga
     7681 acaacaagcg tgagtagccc cgcccaaatt gaggggtcca ttaagaattc catgacaagc
     7741 tcctgcttaa ggaatagcta ttcgacgcca gaaataatgc aggcgtaacg acaaaatgca
     7801 aacgaaaggt gcggcataga gagccagaaa ggcaggcgtt aaaaggcctg atgctgaaat
     7861 gacgtcggtg acgatccata ctgcgggcta ctgccctata ctccatggtt gttaaacggg
     7921 agttaaacat atcagagacg cctctgattt ggcaaagatt taccttcctt tgcaaacgaa
     7981 tgtgacaagg atattttacc tttcgaaatt tctgctaatc gaaagttaaa ttacggatct
     8041 tcatcacata aaataatttt ttcgatatat aaaataaatc gcgaaacgca ggggtttttg
     8101 gttgtagccc ttatctgaat cgattcgatt gtggacgacg attcaaaaat acatctggca
     8161 cgttgaggtg ttaacgataa taaaggaggt agcaagtgac cattgctatt gttataggca
     8221 cacatggttg ggctgcagag cagttgctta aaacggcaga aatgctgtta ggcgagcagg
     8281 aaaacgtcgg ctggatcgat ttcgttccag gtgaaaatgc cgaaacgctg attgaaaagt
     8341 acaacgctca gttggcaaaa ctcgacacca ctaaaggcgt gctgtttctc gttgatacat
     8401 ggggaggcag cccgttcaat gctgccagcc gcattgtcgt cgacaaagag cattatgaag
     8461 tcattgcagg cgttaacatt ccaatgctcg tggaaacgtt aatggcccgt gatgatgacc
     8521 caagctttga tgaactggtg gcactggcag tagaaacagg ccgtgaaggc gtgaaagctc
     8581 tgaaagccaa accggttgaa aaagccgcgc cagcacccgc tgccgcagca ccaaaagcgg
     8641 ctccaactcc ggcaaaacca atggggccaa acgactacat ggttattggc cttgcgcgta
     8701 tcgacgaccg tctgattcac ggtcaggtcg ccacccgctg gaccaaagaa accaatgtct
     8761 cccgtattat tgttgttagt gatgaagtgg ctgcggatac cgttcgtaag acactgctca
     8821 cccaggttgc acctccgggc gtaacagcac acgtagttga tgttgccaaa atgattcgcg
     8881 tctacaacaa cccgaaatat gctggcgaac gcgtaatgct gttatttacc aacccaacag
     8941 atgtagagcg tctcgttgaa ggcggcgtga aaatcacctc tgttaacgtc ggtggtatgg
     9001 cattccgtca gggtaaaacc caggtgaata acgcggtttc ggttgatgaa aaagatatcg
     9061 aggcgttcaa gaaactgaat gcgcgcggta ttgagctgga agtccgtaag gtttccaccg
     9121 atccgaaact gaaaatgatg gatctgatca gcaaaatcga taagtaacgt attgtgttga
     9181 ttatcactca gttttcacac ttaagtctta cgtaaacagg agaagtacaa tggagattac
     9241 cactcttcaa attgtgctgg tatttatcgt agcctgtatc gcaggtatgg gatcaatcct
     9301 cgatgaattt cagtttcacc gtccgctaat cgcgtgtacc ctggtgggta tcgttcttgg
     9361 ggatatgaaa accggtatta ttatcggtgg tacgctggaa atgatcgcgc tgggctggat
     9421 gaacatcggt gctgcagttg cgcctgacgc cgctctggct tctatcattt ctaccattct
     9481 ggttatcgca ggtcatcaga gcattggtgc aggtatcgca ctggcaatcc ctctggccgc
     9541 tgcgggccag gtactgacca tcatcgttcg tactattacc gttgctttcc agcacgctgc
     9601 ggataaggct gctgataacg gcaacctgac agcgatttcc tggatccacg tttcttctct
     9661 gttcctgcaa gcaatgcgtg tggctattcc ggccgtcatc gttgcgctgt ctgttggtac
     9721 cagcgaagta cagaacatgc tgaatgcgat tccggaagtg gtgaccaatg gtctgaatat
     9781 cgccggtggc atgatcgtgg tggttggtta tgcgatggtt atcaacatga tgcgtgctgg
     9841 ctacctgatg ccgttcttct acctcggctt cgtaaccgca gcattcacca actttaacct
     9901 ggttgctctg ggtgtgattg gtactgttat ggcagtgctc tacatccaac ttagcccgaa
     9961 atacaaccgc gtagccggtg cgcctgctca ggcagctggt aacaacgatc tcgataacga
    10021 actggactaa caggtgagcg aaatggttga tacaactcaa actaccaccg agaaaaaact
    10081 cactcaaagt gatattcgtg gcgtcttcct gcgttctaac ctcttccagg gttcatggaa
    10141 cttcgaacgt atgcaggcac tgggtttctg cttctctatg gtaccggcaa ttcgtcgcct
    10201 ctaccctgag aacaacgaag ctcgtaaaca agctattcgc cgtcacctgg agttctttaa
    10261 cacccagccg ttcgtggctg cgccgattct cggcgtaacc ctggcgctgg aagaacagcg
    10321 tgctaatggc gcagagatcg acgacggtgc tatcaacggt atcaaagtcg gtttgatggg
    10381 gccgctggct ggtgtaggcg acccgatctt ctggggaacc gtacgtccgg tatttgcagc
    10441 actgggtgcc ggtatcgcga tgagcggcag cctgttaggt ccgctgctgt tcttcatcct
    10501 gtttaacctg gtgcgtctgg caacccgtta ctacggcgta gcgtatggtt actccaaagg
    10561 tatcgatatc gttaaagata tgggtggtgg cttcctgcaa aaactgacgg aaggggcgtc
    10621 tatcctcggc ctgtttgtca tgggggcatt ggttaacaag tggacacatg tcaacatccc
    10681 gctggttgtc tctcgcatta ctgaccagac gggcaaagaa cacgttacta ctgtccagac
    10741 tattctggac cagttaatgc caggcctggt accactgctg ctgacctttg cttgtatgtg
    10801 gctactgcgc aaaaaagtta acccgctgtg gatcatcgtt ggcttcttcg tcatcggtat
    10861 cgctggttac gcttgcggcc tgctgggact gtaagactgt tgtacactac cggggccttt
    10921 tggccccatt tttttatctg gaggattaat gacaatcacg gacctggtac tgattctttt
    10981 catcgccgca ctcctggcct tcgcgatcta cgatcagttc atcatgcccc gccgtaacgg
    11041 ccccaccctg ctggcaattc ctttgctccg gcgtggtcgc atcgatagcg ttatcttcgt
    11101 cggattgatt gtcattctta tctataacaa cgtcacgaat catggtgcgt taataacgac
    11161 atggttatta agcgcactgg ctctgatggg tttttatata ttctggatcc gcgttccgaa
    11221 gatcatcttt aaacaaaaag gttttttctt cgccaatgtc tggattgaat atagccgaat
    11281 caaagcgatg aacttgtcgg aagatggcgt gttggtgatg caattagaac agcgtcggct
    11341 gttaatccgc gttcgaaata tcgacgatct ggaaaaaatt tataagcttc tcgtttcaac
    11401 tcaataagtt atgaatttag ccaaagctat gtttagtgta tttttaataa tcagacatag
    11461 cttaggctat attacctctt cccttatttg ttatttattt taacgtttca ttgatatata
    11521 aatctaaatg taaatcgtta tcaataaagc aatgaaataa tatattccaa cagttgtttt
    11581 atattctcaa aatatgttaa ggttgcgccc tcatttgggg agtagccgat ttccagattc
    11641 cggaaatgta cgtgtcaaca tactcgttgc aaaacgtggc acgtacggac tgaatacttt
    11701 cagtcaggcg agaccatatg cacatcaatc gctatgcctg catgaacgcg aaaaccatcg
    11761 cggcttgttg ttcatgtatg catgtttgct gggggcgatg atgtgtttta tggatacccc
    11821 ggtcaggacg ttgtcatgaa tatcactgct actgttcttc ttgcgtttgg tatgtcgatg
    11881 gatgcatttg ctgcatcaat cggtaaaggt gccaccctcc ataaaccgaa attttctgaa
    11941 gcattgcgaa ccggccttat ttttggtgcc gtcgaaaccc tgacgccgct gatcggctgg
    12001 ggaatgggca tgttagccag ccggtttgtc cttgaatgga atcactggat tgcgtttgtg
    12061 ctgctgatat tcctcggcgg gcgaatgatt attgagggtt ttcgtggcgc agatgatgaa
    12121 gatgaagagc cgcgccgtcg gcacggtttc tggctactgg taaccaccgc gattgccacc
    12181 agcctggatg ccatggctgt gggtgttggt cttgctttcc tgcaggtcaa cattatcgcg
    12241 accgcattgg ccattggttg tgcaaccttg attatgtcga cattagggat gatggttggt
    12301 cgctttatcg gctcaattat tgggaaaaaa gcggaaattc tcggcgggct ggtgctgatc
    12361 ggcatcggcg tccagatcct ctggacgcac ttccacggtt aataagaacg ctgccagagg
    12421 tgaatattaa agtccgtctg gcagtcgaac acttcttttg ctgccagtgt ttgccagact
    12481 tctggcttcg cacgccaggc aaacggcgtc atctgcaata atgcgacggc ttcatcaccg
    12541 cgaagacgca tcggataaca caactcatca ctctgctgta atgtaaaacc ttccagttgt
    12601 tctgcatgag gtgcatgaag atgtacttca ttgtaaatca gccccttcag ctccatcaaa
    12661 tgtcgcggtc ccggcgtggc agtaatgacc cagccgccgg gcttcactac tcgtgctaat
    12721 tcttctgctt tacacggcgc gtaaatacgt attatggcgt ccatactggt atcggaaaac
    12781 ggcaaacggt ggctggaagc gacacaaaga gtgacctgcg gatagcgttt cgccgccgct
    12841 tttatcgcta ccttcgagac atccaggcca aacgtggtga tttcgggcaa cacatcggca
    12901 aatgcgtgtg tgtaataccc ttcaccacag ccaatatcca gcaccgccgt ggccttttca
    12961 tcaagccgtt cccgcagttg ggcgacaatt gcatcacgca gcggctgata atgtccggca
    13021 tctaagaatg cgcggcgtgc ttgtatcatt tccgcgctgt cgcccggatc acgagaccgt
    13081 ttatgctgaa cgggcagcag attgacatac ccttctttcg ccatatcaaa ctgatgtcgc
    13141 tggggacaga tatagctgtt tttttcacgc gaaagaggct gatggcaaag tggacaggaa
    13201 aacgacatga caactccggc aggatactaa aggccgcaag tgtaacgcga atcacgcggc
    13261 gggcaaatat ccgaacgcca ggagcgaaga taaagcatag taaaaagcct cgcattcgac
    13321 gaggctttat agcgagattg aagcgtattc acacttcaga tcagtggatt cgatcagata
    13381 gctgttacgt taacagctgc cggacctttc tggccgtcct gaatttcgaa ctcaacgttc
    13441 tgaccttcag ccagagtttt gaagccatta ccctggatag cggagaagtg tacgaacaca
    13501 tctttgctgc catcagccgg agtaatgaag ccaaaacctt tagactcgtt gaaccactta
    13561 acctgacctt taatctttgc catttgaaaa attccttaga ttgttttctt cgcccgcagg
    13621 cataacatag ataaaactga cacattactg catgaggcac caatataagg ctcggcagag
    13681 aagcggtatt caacgtcaac gtgtttactc aggacttctt tactgaaaat gccacacata
    13741 aacagaactg tacctcgttt aacccgaaat ctgttatcac atacaacgtt aattatggca
    13801 agccattttt aaacatgtct cgatcagacg cacaaatctc gatacgtctt ccactttttt
    13861 tgcacactta tgcaacggaa tacgcgccga tatatcattg tgcttaacct tgccagttca
    13921 ggcagatact taaactggcg tattttctaa catagttcaa taagttcagt tgttccaggc
    13981 cagtgggaga agttattaca tagtgcgtgc aatatcacat tttttgctat gcaatgaata
    14041 aaaagttata tcactttttc tcataaaaca gtcagttaac ggctatcaat taccctaaag
    14101 agaagtcaat ccccaaaggg attgaaaatt taaaataaga aaaattgatg aatgagcaaa
    14161 aaaatcaaga gagaaacgtt tcgctaataa ataataccac gctt
//
LOCUS       DAZGAN020000122        14018 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGAN020000122 DAZGAN020000000
VERSION     DAZGAN020000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.122"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..105
                     /locus_tag="ACHKYO_RS20255"
                     /old_locus_tag="pgaptmp_004051"
     CDS             <1..105
                     /locus_tag="ACHKYO_RS20255"
                     /old_locus_tag="pgaptmp_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HGU4430813.1"
                     /translation="DNATFRKELEKILRDPELSQALRDELSALVPEQV"
     gene            126..1670
                     /gene="tssC"
                     /locus_tag="ACHKYO_RS20260"
                     /old_locus_tag="pgaptmp_004052"
     CDS             126..1670
                     /gene="tssC"
                     /locus_tag="ACHKYO_RS20260"
                     /old_locus_tag="pgaptmp_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HGU4430814.1"
                     /translation="MLMSVQKENNVAGNAVSETHSGDSVYASLFEKINLNPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGAPVEKLDKALIDHHIAELDYQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAHMPFIGSVGP
                     AFFRKDTMEEVAAIKDIGNHFERAEYIKWNAFRETDDARYIGLVMPRVLGRLPYGPDT
                     VPVRSFNYVEEVKGPDHHKYLWTNASFAFAANMVRSFVTNGWCVQIRGPQAGGAVQDL
                     PIHLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPA
                     LYDTPDATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRLLELELNNWIWGL
                     VTEMTDPGDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQ
                     MPKAKA"
     gene            1686..3023
                     /gene="tssK"
                     /locus_tag="ACHKYO_RS20265"
                     /old_locus_tag="pgaptmp_004053"
     CDS             1686..3023
                     /gene="tssK"
                     /locus_tag="ACHKYO_RS20265"
                     /old_locus_tag="pgaptmp_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGU4430815.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSERSLVDIVLA
                     LPLLNANGGNLDDGSESERPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCRDPRFIPPLLTLSASPSLMTELAELLYHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQNGVMWEGAL
                     HDARLREGADFWLSVRSSIPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            3020..3673
                     /gene="tssL"
                     /locus_tag="ACHKYO_RS20270"
                     /old_locus_tag="pgaptmp_004054"
     CDS             3020..3673
                     /gene="tssL"
                     /locus_tag="ACHKYO_RS20270"
                     /old_locus_tag="pgaptmp_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HGU4430816.1"
                     /translation="MNKPVISRAEQIFYPGWLMVSQLRCGQPVEDGKALYRRACQLVT
                     QAREELAEAEFSQESSEIMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRVGRTMYWLSWVAGIVTLAGLWCVLSSVLADQVARLTGQG"
     gene            3676..5376
                     /locus_tag="ACHKYO_RS20275"
                     /old_locus_tag="pgaptmp_004055"
     CDS             3676..5376
                     /locus_tag="ACHKYO_RS20275"
                     /old_locus_tag="pgaptmp_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU4430817.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWRCLSSRRDIPENAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLRECDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     ARPGNLWQQQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSQLLNDWQ
                     QRGVPQRYGLGLYQGMWLIPPVERATGEWIAPLPPRPVIQKVTQTPKMVRLNSLALFD
                     AGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPQRNQALSLKRAEAVRDW
                     MRDTGDIPQSCFAVQGYGESRPVAPNETAEGRARNRRVEISLVPQADACRLPDITRAP
                     "
     gene            5411..5902
                     /gene="hcp"
                     /locus_tag="ACHKYO_RS20280"
                     /old_locus_tag="pgaptmp_004056"
     CDS             5411..5902
                     /gene="hcp"
                     /locus_tag="ACHKYO_RS20280"
                     /old_locus_tag="pgaptmp_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HGU4430818.1"
                     /translation="MAIPVYLWLQDDGGADIKGSVDVKDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTRIHTPFLFTKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYF
                     NTKLENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNER
                     TTA"
     gene            6070..8757
                     /gene="tssH"
                     /locus_tag="ACHKYO_RS20285"
                     /old_locus_tag="pgaptmp_004057"
                     /gene_synonym="clpV"
     CDS             6070..8757
                     /gene="tssH"
                     /locus_tag="ACHKYO_RS20285"
                     /old_locus_tag="pgaptmp_004057"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HGU4430819.1"
                     /translation="MENPAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLL
                     EQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRSRPQLAQSLNALLKQAWMVAS
                     LQGEEHIRSVHLLGALTENPHLVRCDGVWPLLTLSQSQLQRLSPLLDAQSDECPERLQ
                     DAEPVLPQGDNVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRDITASAREGKIDPV
                     SGRDTEIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLR
                     TLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGGADAA
                     NLLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVRVDEPDDETACLMLRSLK
                     SRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQL
                     TRIRSQLASLGMEKQALLEDIAVGHQNHGERLSAIEQEENVLMTARDDLEQQYARECE
                     LTGELLESRSDISRQSETLHLQQALHDIQQNQPLLSVDVDVRTVAGVVADWTGVPLSS
                     LMKDEQTELLHLEKDIGRRVVGQDVALGSIAQRLRAAKTGLTSGNGPQGVFLLVGPSG
                     VGKTETALALADVMYGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAV
                     RKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMRDGEGREIDFRNTVILMTSNLGSDL
                     LMQQLSEKPETTESELHELIRPLLRDHFQPALLARFRTVIYRPLTQSAMRIIVEMKLA
                     QVCERLHCHYGLSTSVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSYL
                     AARQHPTYLSLTWHDEDGIVIAFDGINSDYPQESIYGQC"
     gene            8744..9385
                     /locus_tag="ACHKYO_RS20290"
                     /old_locus_tag="pgaptmp_004058"
     CDS             8744..9385
                     /locus_tag="ACHKYO_RS20290"
                     /old_locus_tag="pgaptmp_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430820.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTGHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            9688..12207
                     /locus_tag="ACHKYO_RS20295"
                     /old_locus_tag="pgaptmp_004059"
     CDS             9688..12207
                     /locus_tag="ACHKYO_RS20295"
                     /old_locus_tag="pgaptmp_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HGU4430821.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATLTMGTGGLKGQVNQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEIVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKITGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLEQALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            12204..13886
                     /gene="tle1"
                     /locus_tag="ACHKYO_RS20300"
                     /old_locus_tag="pgaptmp_004060"
     CDS             12204..13886
                     /gene="tle1"
                     /locus_tag="ACHKYO_RS20300"
                     /old_locus_tag="pgaptmp_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HGU4430822.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNNISNGSYRGYYSNI
                     HWLNILYHPDQVLKKDQTSAQIKTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSIA
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PAFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFAPIDPKNPDMNLSPE
                     LIPFVDKAIAQGKAVRLGKEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
     gene            13906..>14018
                     /locus_tag="ACHKYO_RS20305"
                     /old_locus_tag="pgaptmp_004061"
     CDS             13906..>14018
                     /locus_tag="ACHKYO_RS20305"
                     /old_locus_tag="pgaptmp_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HGU4430823.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYK"
BASE COUNT         3304 a         3228 c         4044 g         3442 t
ORIGIN      
        1 gataacgcca ctttccgtaa agaactggaa aaaattctgc gggatcccga actgagtcag
       61 gccttacgtg acgaactgag tgcactggtg ccggaacagg tgtgattaac ggattaagag
      121 agagcatgct gatgtctgta cagaaagaaa ataatgtggc gggaaatgcg gtttctgaaa
      181 cgcattccgg cgacagcgta tatgcttccc tgtttgaaaa aattaatctg aatccggtat
      241 cagccctgag tgcgctggat atctggcagg atccgcaggc catgtcagag gcatcagcag
      301 atgaacgtct gaccgcaggg atgcaggtgt ttatggaatg cctggcgaaa gcgggagcac
      361 cggtggagaa actcgataag gcgctgattg accatcatat tgcagagctt gattatcaga
      421 tcagccgtca gctggatgcc gtgcttcatc atccggaatt tcagaaagtg gagtccctgt
      481 ggcggggcgt gaagtctctg gtggataaaa cggacttccg ccgtaatgtg aaaattgaac
      541 tgctggatct gtccaaagac gatctgcgtc aggattttga ggatgcgccg gaaattattc
      601 agagtggtct ttatctgcag acttatgtgg cggagtacga tacgccgggt ggtgaaccca
      661 ttgcagccct gatttctgcc tgggagtttg atgcgtctgc gcaggatgtg gcgctgctga
      721 aaaatatttc ccgggtggct gcgtccgcgc atatgccatt tattggttca gttggcccgg
      781 ctttcttccg gaaggatacg atggaagagg tggccgccat taaggatatt ggcaatcact
      841 ttgagcgtgc cgagtacatc aagtggaatg ctttccgtga aacggatgat gcccgctata
      901 ttggtctggt gatgccacgt gttctgggac gcctgccata tggtcctgat accgtacctg
      961 tccgcagctt taactatgtt gaagaggtga aaggtccgga tcatcataag tatctctgga
     1021 caaatgcgtc ttttgctttt gcggccaata tggtgcgcag ttttgtcacc aacggctggt
     1081 gtgttcagat ccgcggtcct caggccggcg gtgcggtgca ggatttgccg atccatctgt
     1141 atgatctggg aaccggtaat caggtcaaaa ttccgtcaga ggtgatgatt ccggaaactc
     1201 gggagtttga attcgctaat ctgggcttta ttccgctctc ttactacaag aaccgggatt
     1261 atgcctgctt cttctccgct aactccaccc agaaaccggc actgtacgat acgccggatg
     1321 caacggcgaa cagccgtatt aatgcccgtc ttccctatat cttcctgctg tcccgcattg
     1381 cgcattacct gaagatcatt cagagggaga atatcggtac caccaaagat cgccgtctgc
     1441 ttgaactgga actgaataac tggatctggg gtctggtgac ggagatgacc gatccgggtg
     1501 atgaactgca ggcctcacat ccgctgcgtg acggaaaagt ggtggtggaa gacatcgaag
     1561 acaacccggg atttttccgc gtgaaactct atgctgtacc acatttccag gtggaaggta
     1621 tggacgtcag tctgtcactg gtttcgcaga tgccgaaggc aaaagcgtaa ggcaggcgtc
     1681 gggaaatgaa gatttatcgt ccactgtggg aagacggggc ttttctgatg ccccagcagt
     1741 ttcagcagca ggctgcctgg gatgttcatc tggcagacag cgttgcccga atggggctgg
     1801 cgcatccctg gggcgtggtg gctgcagagt ttgatgactc tctgttaccg ctttcacggt
     1861 tgaatgccac ccgtctgatt gttcgtttcc cggacggaac gcttattgat acggaacgtg
     1921 cggataatct gcctccggtc tgtgatttat cgacggtttc tgagcgttcc ctggtggata
     1981 ttgtgctggc gctaccgtta ctgaatgcta acggtggcaa ccttgatgat ggcagtgaga
     2041 gcgagcgtcc gcgtcgctgg aaatcagaaa gggtgaatgt gcaggagctg gccgggcatg
     2101 agcaaagcga agtggcggta ttgcggcata acctgactct gcgcatggct catcaggaaa
     2161 atgctgcctg gctgacctgt cctgttaccc gtcttgttcg agatgcacag ggacagtggt
     2221 gcagggaccc gcgttttatt ccccctctgc tgacgctgtc tgccagtcct tcgctgatga
     2281 ctgagcttgc ggagttgctt tatcacctgc aggcgcgtcg ccagcgactg atgtccatgc
     2341 gtcgggagaa caatgcccgt cttgcggatt ttgcagtggc ggacgtgtcg ttgttctggt
     2401 tgctgaatgc cctgaacagt gcggagcctg ttctgaaaga gttgcttgac atgccttacc
     2461 gccacccgga actgttgtac agggagcttg cccggctggc aggctccctg ctcacattct
     2521 cgcttgagca taatgtggat gcggttccgg cataccgtca tgaaacacct gaaaatgtgt
     2581 ttccaccctt gctttctttg ctgaaccagc tgctggaggc aagcctgcct tcccgtgtgg
     2641 ttttcattga actgaaacaa aatggtgtga tgtgggaggg ggcattacat gatgcgcgtt
     2701 tgcgcgaagg ggcggatttc tggctttcag tgcgctcttc catacccggg catgaactgc
     2761 agacgaagtt tccgcagtta tgcaaagccg ggagccccga tgatgtgtct gaggtggtga
     2821 atgtggcgct gagcggggtc attatccgtc cggttacgca tgtaccggcg gccattccgc
     2881 tccggctgga aaaccagtat ttcgcgctgg atttaagtac ggatgcagcc cgggcgatgc
     2941 tggacgcggg gcgttgtact ttctacaccc cggcatcgct gggggatgtg aaactggaac
     3001 tttttgcggt actgcggaca tgaataaacc tgttatctcc cgggctgaac agatttttta
     3061 tcccggctgg ctgatggtca gccagctgcg ctgcggacaa ccggttgagg atggtaaggc
     3121 cctgtatcgc cgtgcctgtc agctggtgac gcaggcgcgt gaagagctgg ctgaagcaga
     3181 attcagtcag gaaagcagtg aaatcatgtt gtatgccttc tgcgccctgc tggacgagag
     3241 tgtgctgaac cgtgaaaaaa cagacgatgg ctggcacacc tggcagcagg acccgctgca
     3301 ggctcatttt tttggtacgc tcaatgccgg agaagagctc tgggagcgta ttcgtgagca
     3361 actgaaatta cctgcgccgg atgtggcggt actgacatgc ctctgccgta cgcttcagct
     3421 cggttttacc ggtcagtacc ggtcgcagga tgatgagcgt cgcgaagatg taattcgcgc
     3481 actgacagcg cgtgttcctg catttacttt cgcacaggat gcgccggtgg tggtgcgtgc
     3541 accgggttac cgtgttggca gaacgatgta ctggttgtca tgggtggcgg gtattgtgac
     3601 gctggctggt ctctggtgtg tgctttcttc tgtgctcgca gaccaggtgg cacggcttac
     3661 cgggcaggga taaccatgca cgtacgttcc cgtctctggc tgacaatact gagtctgttc
     3721 ctgacactgt ggctgatttc gggattcctg ccactgtctg cagtaaacca gataatactg
     3781 accctggttt cagttgtggc ggcggggctt gtgctgtggt atcagtggcg ttgtctgtct
     3841 tcccgtcgtg atatcccgga gaatgccggc gatgacggtc tgccgcctga gtattttcag
     3901 gggcatgtgt tgcttgttgc cggaaactca gatgcgtggt tcgaacccgg tcaggcatac
     3961 cgggaaacag catcgggatg gtatcttcgt gtggatacgc cggagcagat gagaatactg
     4021 gcagagcggt tgtcgtacag ccgtccggcc ctgtgtggcc aggtcgtggt catgctggga
     4081 cttttaccgg agcagcatac ctcagatgaa gctttcctgt cgtggttgcg gaactggcag
     4141 cgtgcagtca tgcagtgccg gcagtggctg aataccatgc cgccggtgct ggtgacattg
     4201 atggtttcag ggccgtcacc acaaaacaca tccttaaata agcactctgc acactggttt
     4261 acactgacac cggacctgtc gggaatgcac atacgcctga cggaagccgg agccattccg
     4321 gcagaggaat ggctccggga gtgtgatgca gcgtccaggc tccggtgcgt cagtgaactg
     4381 ttatggctga atgccatgtt gtcctggtac aaccgggtta ctgatgccct gacagatatt
     4441 tgccggcagg agcactttta tttccggccg gttgccgttg gattctgtct gacgtcggtt
     4501 gcggcccggc ccggtaatct ctggcaacaa caactgacgt cactcactgt actgccaccg
     4561 cctgccggcg tgtcatcaca ggcactgccg ttgccggata ttctgctgtc cggtattccc
     4621 cgtcatcgtg gactcagtca ctggcacaga ctttgtctcc agacgggggc gattgtcggc
     4681 gtttttcttc tgctggctat gctggcgtct tttatgaata accagcgact ggttcgcagc
     4741 gtgggggaac acctgacggc ctggcaccgg atgcgcggat attcatctga atcgaaaaaa
     4801 actgcactgc aacgtcttca ggctgacagc cagttactga acgactggca gcaacgtggt
     4861 gtgccgcagc gttacggact gggattgtat cagggaatgt ggctgatacc gccggttgag
     4921 cgagcgactg gtgagtggat tgcaccactg cctccccgtc cggtaataca gaaagttact
     4981 cagacgccga aaatggttcg ccttaacagc ctggcgctgt ttgatgccgg taaatggacg
     5041 ctgaaaccgg gcgccacaaa atggctggtg aatgccctgg tggatattaa ggcaaaagcg
     5101 ggctggctga ttgtggtcag tggtcacacg gacaatacgg gcgacccgca gcgcaaccag
     5161 gccctgtcac tgaagcgcgc agaggccgtt cgcgactgga tgcgggatac gggcgatatc
     5221 cctcaaagct gttttgccgt acagggatat ggtgagagtc gtcccgtggc tccgaatgag
     5281 acggcggaag ggcgagcgcg taaccgccgt gttgaaatca gtctggtacc gcaggccgat
     5341 gcctgtcgtc tgccggacat cacccgggcg ccgtaacagg gcgcgtaaga caacgagagg
     5401 gagtaacttt atggcaattc ctgtttatct gtggctgcag gatgatggcg gagcggatat
     5461 taaaggctcc gtggatgtga aggaccgtga gggcagtatt gaagtggtgg ctcaggagca
     5521 ctgcctttac attccgacgg ataacaatac cggtaagctg accggtaccc gtattcacac
     5581 gccgttcctg tttaccaagg aaattgattc ctccagcccc tacctttaca aggcggtgac
     5641 cacggggcag accctgaagt ctgctgaatt taagtggtac agaatcaatg atgccggtca
     5701 ggaagtggag tatttcaata cgaagcttga gaacgtgaag gtggtgaagg ttaacccggt
     5761 catgcacgac atcaagaatc ccgcctacga gaagcataac caccttgagc aggtggagct
     5821 gcgctacgag aaaatcacct ggacttataa ggacggtaac atcattcatt ctgacgcgtg
     5881 gaatgaacgt accactgcgt aattctcccg gacgggcagg gtatcctgtc cgtcattttc
     5941 ttttttcttc agtatctgtt ggcgggtgct gaagaaagaa ttctcaatcc tgaacacaga
     6001 cctgatgcgc ccgggttatg gcgaagggcg gtagcgtgtc tgaaggtctt ctgacagtga
     6061 gtgaataaca tggaaaatcc ggcagccctg ttacgtcgtc ttaatcatta ctgtgcccgt
     6121 gcactggaag gcgcagcctc cctttgccag acccgggccc atgcggaaat caccccggag
     6181 cactggttac tgaaactgct ggagcagggg gaaggagacc ttaccgtgct gggccggcgt
     6241 tatgactggg atatggacgc aatatggcag tcgctgctcg gctggctgga taaccagccc
     6301 cgtagcgtac gcagccgccc gcagcttgcg cagtccctga atgccctgct gaaacaggcc
     6361 tggatggtgg cctcattaca gggggaagaa catatccgca gcgtgcatct gctgggtgcc
     6421 ctgacggaaa atccgcacct ggttcgctgt gacggggtgt ggcccctgct gacactgagt
     6481 cagagccagt tgcagcgtct gtccccgctg ctggatgcgc agtctgatga gtgtccggag
     6541 agattacagg atgcagagcc tgtgctgcct cagggagaca atgtgacctt tatcgggcgt
     6601 cccgtcggtg cggatacggc gggtataccg tcaggtgacc tgccgcctgt gttacagagt
     6661 gcgctggata aattcacccg ggacatcacg gccagcgcga gagaggggaa aattgacccg
     6721 gtatcgggac gcgatacgga aatccgtcag atggtggata ttctctcccg ccgccgcaag
     6781 aacaacccta ttctggtggg agaccccggt gtggggaaaa cggctctggt ggaagggctc
     6841 gccctgcgta ttgcggaagg taacgtaccg gaatcactca gacccgtcac cctgcgtacc
     6901 cttgacctcg gcctgctgca ggccggtgcc ggcgtgaagg gtgaatttga acagcgcctg
     6961 aaaaacgtga ttgatgccgt gcagcagtca ccggctcctg tactgttgtt tatagatgaa
     7021 gcccataccc ttatcggggc cggtaatcag gccggcggcg cggatgcggc caacctgctg
     7081 aagcctgcgc tggcgcgggg tgaactgcgt accattgccg ccaccacctg gtctgagtac
     7141 aaacaatact ttgaacgtga cgcggctctg gagcggcgtt ttcagatggt cagagtggac
     7201 gagccggatg atgagacggc ctgtctgatg ctgcgctccc tgaaatcccg ttatgcggaa
     7261 catcataacg tgcatatcac cgatgaggcg gtacgtgctg ccgtcacgct gtcgcgccgt
     7321 tatctgacag aacgccagtt accggacaag gccgttgacc tgctcgatac tgctgctgcc
     7381 cgtgtgcgta tgagcctcga cacggtgccg gaacagctga cccgtatccg ttcacagctc
     7441 gcttccctcg gtatggaaaa acaggcactg ctggaagata ttgccgttgg tcatcagaat
     7501 cacggcgaac gtctgtctgc cattgagcag gaagagaatg tgctgatgac agcacgtgat
     7561 gacctggagc agcagtacgc ccgggagtgc gaactcaccg gtgagttact tgaaagtcgc
     7621 agcgatattt cccgtcagag tgagacactg catctgcagc aggcactgca cgacattcag
     7681 cagaatcagc ccctgctcag tgtggatgtt gacgtacgca ccgttgccgg tgtggtcgcg
     7741 gactggaccg gggtgccgtt atcttccctg atgaaggacg aacagacgga actgctgcat
     7801 ctggaaaagg atatcggcag acgggtggtc ggacaggacg tggcactggg atccattgcg
     7861 cagcgtctgc gtgcggcgaa aacgggtctg acatccggta acggccccca gggcgtgttc
     7921 ctgctggtcg gtcccagtgg tgtgggaaaa accgaaacgg cactggcgct ggcagatgtg
     7981 atgtacggcg gcgaaaaatc actgattacc atcaatctgt cggaatacca ggaaccccat
     8041 acggtttccc agcttaaggg ttcaccgccc gggtacgtgg gatacggtca ggggggtatt
     8101 ctgacggaag ctgtacgtaa gcgtccgtac agtgtggtgc tgctggatga agtggaaaag
     8161 gcccaccggg atgtgctgaa tctgttctac caggtgtttg accggggctt tatgcgcgac
     8221 ggtgaggggc gtgaaattga cttccgtaat acggtcattc tgatgacctc caatctgggc
     8281 agtgaccttc tgatgcagca actgagcgaa aagccggaga caacggaatc ggagctgcat
     8341 gagcttatcc gtccactgct gcgcgaccac ttccagccag cactgcttgc ccgtttccgg
     8401 accgtgattt accgtccgct gacgcagtct gccatgcgca tcattgtgga gatgaagctt
     8461 gcacaggtct gtgaacgcct gcactgccat tatgggctga gcacatcggt tgatgaacgt
     8521 gtgtacgatg ccctgacatc cgcctgcctg ctgccggata cgggcgcccg gaatgtggag
     8581 agtctgctga accagcagct gctgccggta ctgagccggc agttgctcag ctatcttgct
     8641 gccagacagc atccgacgta cctgtctctg acgtggcatg atgaggatgg tattgtgata
     8701 gcgtttgatg gaatcaatag cgattatccg caggaaagca tatatggaca gtgctaaaga
     8761 aattgcccgg gatatgctgg aggccgtaaa tattaatggg gatgggtttc tgggggccgt
     8821 cgggaaggga cttatatctt tgccagtcag tctcggttat atgggatatg attttataga
     8881 taccgggcat aggcgggaaa accagaatga caaattcaga atggccagac tggtagaaaa
     8941 aggcattttc agccgtgata ttattgggaa aattatcagt atttgtcttg aagactttgt
     9001 atcccggatc aatatggaaa aaacaggctc cattgtgaag aacgtttcag gagatctggc
     9061 aggtagaatg gcatttgctg aattgaccgg ggttaaactg ggagaggttc tggtatccag
     9121 agggctggct gctttttttg cagggagttt tgccgggttg ttattatctg ttggcgcaga
     9181 gacatcgcgt gcgatttaca catcccgttt tctgaaagac agaaatccgg tgttgcatga
     9241 taagctgaaa gctcaggggg atttggatct tttgtacttt cttgttgaag atattgtgcg
     9301 cccgtttgaa cgagcctgta ttgtcgcaga ccgaaattcg gctgagtttg accggatatg
     9361 cgaatatttt ttcggtggtt tatgaagata aaaagaacat tacttataat gctttcccga
     9421 tttatccgtg gcgcaggtat ggggttaggt gcgtcgggta ttgtatttac agtctggttc
     9481 ttttttctct catctgctga atttaaatat ctgtggggag ggctctctgt tgcgttattt
     9541 tttgtcggtt atctgatgta tcgatttgca tatgcatata tttacgatga atgggatgat
     9601 tatcagtaat tctctctgaa aataaagctt aacggccatc attatataat tgcagttcaa
     9661 taccggtaca gacatggagt taacaatatg agtctgacag atacactttc aaaaactatc
     9721 tctgcttccc tgaatcgtta ccatttaatt attccctcct gcccgtcacc actggatgtt
     9781 gagcatttca gcggcagaga ggagatgagt cagacctatt attacatcat caattttacc
     9841 agtactgaca acgacctgga tgccgggctg ttgctgcgta aatcagccac attgacaatg
     9901 ggtacgggag ggcttaaggg gcaggtgaac cagaaagtag tacatggtgt cattacagat
     9961 tttcgccgca tatcgggctc cgccgaccag gcaaaatatc aggtgatact ggaaccattt
    10021 attaaactac tggacaggca gcatcgttct caccgatttt tcgttaacaa atcggtgccg
    10081 gaggtggtga cagaggttct ccaggagcac ggcctgaaag gctgggagtt tgagttccgt
    10141 ctcaaaaaaa cgtatccgaa gcgtgaacaa attaaccagt atcaggaaag tgacctgcaa
    10201 tttattcagc gtttgctggc tgaagtgggg attttttatt ttttcaccct gcaaccggac
    10261 acgcagacgg aaatagtgca ttttggtgac agccaggctg cgctgacgtt tgataagacg
    10321 ctggcgataa acagtccgtc cggaatgaat gataacagag cggattcagt gtgggggctg
    10381 aatgtgacgc acaatgtggc agaggccagt gtcaccacaa aggcttataa ccatcgtgaa
    10441 gcacaatatc tgttgcagtc cgccccggca gacatgacgc atggtgatgg tgacggaatt
    10501 aactatggtg aggtgtacca ctaccatttc cgtcatctgg aacgggggga taaaatcgac
    10561 ccggtgccgg agacggccaa tttttatgcc cgccttggtc atgaacgtta tctggcggag
    10621 caggtacgga taaccggcaa cagcacagac gccacgctgg cgcctgctca ggttctgact
    10681 atcaccgaca gtcttccgcc gacgttgcct gcgctgctgc gcaatccggt gttgcttacc
    10741 tgtgtcggtt tttctgcaag tcgcaaggat gccctgcagg ttgtactgaa gggcgttcct
    10801 tacagtgaag ttatctgctg gcgaccaccg ttacttcctc gtccgaaaat caccggtacc
    10861 atgacggccc gtgtgaccag tgcaaaagaa ggggatatct acgcctggca ggatgcatca
    10921 ggcatgtacc gggtgaaatt tgatgctgac cgtgatgata aaaatccggg acaggaaagc
    10981 atgccggtac gtctggccaa accctacagt ggtgatgctt atggttttca ttttccgctg
    11041 attcagggaa cggaagtggc tattgccttt gaggaaggtg atccggatcg cccctatatc
    11101 gcgcatgccc tgcatgattc acgtcatgtt gatcacgtta ccgataagaa cggtacgcgc
    11161 aatgttatcc gtacgccggc aaataacaag ttgcggatgg aggacaaacg cggtgaggag
    11221 catatcaaga tcagtaccga gtttggcggt aagacacaac ttaatcttgg tcataatgtt
    11281 aatgcctccc ggaaactcag gggagaaggc gctgagctga ggactgatga ctggataagt
    11341 atccggggag gaaaaggaat atttatcagt gcagatatgc agcctcaggc acaaggaaag
    11401 atgctggata tgaatgaagc tatccgccaa ctggagcagg cattatctct ggcccgaagt
    11461 atggcgaagg ccgcaactgc tgcaaatgct actcagggag atataagttg tcagcaacgg
    11521 ttgaattcat cgctaacaga tctcactgcg ccgggaatgc tgttgcatgc tcctgatggg
    11581 attggcatgg ttagtgcacg ggcgttacga attgcttcgg gtagtgaaag cgtgggcata
    11641 atgtcgggag ataacactga tattagtgcc gggcagtcat ttacagttgt agcagaaggt
    11701 gctgttagcc tgttgagcag aaaccagggg atgcaactgt tagcagcaaa agggcgggta
    11761 aatattcagg ctcaaagtga cgatttgtca atgagttccc aacagaatct tgatattcaa
    11821 agttctgagg gcaaagtgac tgtttctgct aatcaggaac tgatactggc atgtggtgga
    11881 gcttatatca aactaagcgg tggaaatatt gagctgggat gcccgggaca gatcctgtta
    11941 aaaagcacga atatgcagaa gatgggacca acaagtcttg atattgcgtc tgttgaaatg
    12001 ccgcgagggt ttggtggcgg ttttattctt accgatgaag cgggagtgcc gcaaccttcg
    12061 acgccttatc gattaacaac ggctgaaggt gatattctac aggggatcac cgatgaaaat
    12121 ggtaaaacag cgccagtcaa cacctccatt ccgtcagtag taaaagttga gtttgggaag
    12181 gtaaaaattc atggagaaac agaatgacaa aatatcaggg atatgacgta acagatgcca
    12241 cccataaaac cagtattcat aatgactgga aagttgttgt tgcaaagaaa aaaccggcgc
    12301 ggggtgtgac tttaaccatt ggtatatttt tcgatggtac cggaaacaat cgggagaata
    12361 cagcatctcg tctgatgaaa tttaatgaat gtagtgcagc cagacagggg gtaaatcaaa
    12421 aagatgcaca aagctgtgag gattttcttg aagaaattaa caagaacaat atctcgaatg
    12481 gcagttatcg tggatattat tccaatattc actggcttaa tatactctat catccagatc
    12541 aggtacttaa aaaagaccag acgtcagctc aaattaagac ttacatttcc gggataggga
    12601 ctgcggctgg tgaagcagat tcagtcattg gcatgggatt aggcaccagt attctggata
    12661 tatttgaagg tgtggtgacg aaaaccgacg aggcaatgga acgaatcacc caggcgttat
    12721 cagaatttat ggggttcaat ttaaatcctg atttttgtat cgcaaaaatc cagtttgatg
    12781 tttttggttt tagccgggga gcagcggcgg caaggcactt tgctaatcgg gttatggaac
    12841 aggatcctgc tatagccaga gctattgcta aagggctcag aggcgatttt tatgatggta
    12901 aaccttcggg tgaagtccgt tttctggggc tttttgatac tgtcgcagct attggtggaa
    12961 tcagtaattt cttcgatatt aatggacgta gcaacccggg agtaaagctg gaattgcgtc
    13021 cttccatcgc gaagaaagtt ttccagatta ctgcaatgaa tgaataccga tataatttca
    13081 gcctgaacag tattaaaggt atgtggcccg aactggcttt accaggggcg cattcagata
    13141 ttggaggtgg ttacaatccg gtaggttcac ctctgcagga aaatgaaagt ctgtttctga
    13201 gttgcccggc atttgaaatt gtcagtgacg ataccagaga aacggatacg cgagtatacc
    13261 gtaatgcaga gcaagccaga aaaatgttga tgactctgcc agccttaaaa catattttgc
    13321 ctcatggaaa actaaccacc aaaattcgtt ccgtaggtgt gaacaacagc aatcaacgtc
    13381 gggctggcgt gatacaaaag caggtgggag ccgcagtatt ttttgagcgt atggcagtac
    13441 ctaatgactg ggctaatgtt tgcctgcgtg tgatgctgga tgctgctcag gaagcagggg
    13501 tattgtttgc tccaattgac cccaaaaatc cagatatgaa tttatctcct gaattaattc
    13561 cttttgttga taaagccatt gctcagggaa aagcggtacg tttggggaaa gagcctcagg
    13621 cttttactga ggaagagttg tatattatcg gaaaatacac ccattgttct gccaactgga
    13681 atattgagtc tgatggtaat ttatgggtgg atcctacaac gggagagatt tttatccatc
    13741 gctttggtcc taaaggtaat aaggcgttcg tttttccgaa taaaccaaat gatcgctgga
    13801 ttcgttctgt atggtacatg gatgatcagc agcgattgaa tgataacgca gtaaaaaata
    13861 ctaaagttat gatgtctggt gtttaacgct acaggatgac aatacatgac cagagataaa
    13921 atcattttta tcgcaccatt attggcattg ttaactgggt gtaattctgt tagctcacag
    13981 tatgctgatt cggataagac tgaatggggt tataaaaa
//
LOCUS       DAZGAN020000123        13848 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGAN020000123 DAZGAN020000000
VERSION     DAZGAN020000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.123"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            171..884
                     /gene="aphA"
                     /locus_tag="ACHKYO_RS20310"
                     /old_locus_tag="pgaptmp_004062"
     CDS             171..884
                     /gene="aphA"
                     /locus_tag="ACHKYO_RS20310"
                     /old_locus_tag="pgaptmp_004062"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HGU4430824.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            995..1411
                     /locus_tag="ACHKYO_RS20315"
                     /old_locus_tag="pgaptmp_004063"
     CDS             995..1411
                     /locus_tag="ACHKYO_RS20315"
                     /old_locus_tag="pgaptmp_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HGU4430825.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1415..1771
                     /locus_tag="ACHKYO_RS20320"
                     /old_locus_tag="pgaptmp_004064"
     CDS             1415..1771
                     /locus_tag="ACHKYO_RS20320"
                     /old_locus_tag="pgaptmp_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU4430826.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(1806..4628)
                     /gene="uvrA"
                     /locus_tag="ACHKYO_RS20325"
                     /old_locus_tag="pgaptmp_004065"
     CDS             complement(1806..4628)
                     /gene="uvrA"
                     /locus_tag="ACHKYO_RS20325"
                     /old_locus_tag="pgaptmp_004065"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGU4430827.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            4882..5418
                     /gene="ssb1"
                     /locus_tag="ACHKYO_RS20330"
                     /old_locus_tag="pgaptmp_004066"
     CDS             4882..5418
                     /gene="ssb1"
                     /locus_tag="ACHKYO_RS20330"
                     /old_locus_tag="pgaptmp_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HGU4430828.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5517..5798)
                     /locus_tag="ACHKYO_RS20335"
                     /old_locus_tag="pgaptmp_004067"
     CDS             complement(5517..5798)
                     /locus_tag="ACHKYO_RS20335"
                     /old_locus_tag="pgaptmp_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HGU4430829.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6228..7814
                     /gene="pdeC"
                     /locus_tag="ACHKYO_RS20340"
                     /old_locus_tag="pgaptmp_004068"
     CDS             6228..7814
                     /gene="pdeC"
                     /locus_tag="ACHKYO_RS20340"
                     /old_locus_tag="pgaptmp_004068"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HGU4430830.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(7817..8140)
                     /gene="soxS"
                     /locus_tag="ACHKYO_RS20345"
                     /old_locus_tag="pgaptmp_004069"
     CDS             complement(7817..8140)
                     /gene="soxS"
                     /locus_tag="ACHKYO_RS20345"
                     /old_locus_tag="pgaptmp_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HGU4430831.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8226..8690
                     /gene="soxR"
                     /locus_tag="ACHKYO_RS20350"
                     /old_locus_tag="pgaptmp_004070"
     CDS             8226..8690
                     /gene="soxR"
                     /locus_tag="ACHKYO_RS20350"
                     /old_locus_tag="pgaptmp_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HGU4430832.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9236..10585
                     /gene="ghxP"
                     /locus_tag="ACHKYO_RS20355"
                     /old_locus_tag="pgaptmp_004071"
                     /gene_synonym="yjcD"
     CDS             9236..10585
                     /gene="ghxP"
                     /locus_tag="ACHKYO_RS20355"
                     /old_locus_tag="pgaptmp_004071"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HGU4430833.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            10737..12386
                     /locus_tag="ACHKYO_RS20360"
                     /old_locus_tag="pgaptmp_004072"
     CDS             10737..12386
                     /locus_tag="ACHKYO_RS20360"
                     /old_locus_tag="pgaptmp_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HGU4430834.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12540..13832)
                     /locus_tag="ACHKYO_RS20365"
                     /old_locus_tag="pgaptmp_004073"
                     /pseudo
     CDS             complement(12540..13832)
                     /locus_tag="ACHKYO_RS20365"
                     /old_locus_tag="pgaptmp_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         3303 a         3321 c         3549 g         3675 t
ORIGIN      
        1 caaaaaaaga caggaacgta tttactgggt taaacataat catcctgctt ttcatcacaa
       61 aaaccgcaga taatccttcc tttccccggc agctggcgtt atggtcagat ggtttttgca
      121 acaaatctca caataaaaag tttcaacata ctgactattt agggaaaaat atgcgcaaga
      181 tcacacaggc aatcagtgcc gtttgcttat tgttcgctct aaacagttcc gctgttgccc
      241 tggcctcatc tccttcaccg cttaaccctg ggactaacgt tgccaggctt gctgaacagg
      301 caccaattca ttgggtttcg gtcgcacaaa ttgaaaatag cctcgcaggg cgtccgccaa
      361 tggcggtggg gtttgatatc gatgacacgg tacttttttc cagcccgggc ttctggcgcg
      421 gcaaaaaaac cttctcacca gaaagcgaag attatctgaa aaatcctgtg ttctgggaaa
      481 aaatgaacaa tggctgggat gaattcagca ttccaaaaga ggtcgctcgc cagctgattg
      541 atatgcatgt acgccgcggt gacgcgatct tctttgtgac tggtcgtagc ccgacgaaaa
      601 cagaaacggt ttcaaaaacg ctggcggata attttcatat tcctgtcacc aacatgaatc
      661 cggtgatctt tgcgggcgat aaaccagggc aaaatacaaa atcgcaatgg ctgcaggata
      721 aaaatatccg aattttttat ggcgattccg ataacgatat taccgccgca cgcgatgtcg
      781 gcgctcgtgg tatccgtatt ctgcgcgcct ccaactctac ctacaaaccc ttgccacaag
      841 cgggtgcgtt tggtgaagag gtgatcgtca attcagaata ctgacagagc gggagagcgt
      901 gacgctctcc cgaatgctgt ttttttaatc acacctttat cctttcgctg tcttgctgca
      961 aactgattaa gagagtttta tcaaggagca gcacatgtgg tatcaaaaga cgctcacgct
     1021 tagcgccaaa tctcgtgggt ttcatctggt aacggatgaa attctgaatc agctggctga
     1081 tatgccgcgc gttaacatcg gcttactgca tctgttgctg caacatacct ccgcctctct
     1141 gacacttaat gagaactgcg atcccaccgt acgccacgac atggagcgtt ttttcctccg
     1201 caccgttccc gacaacggaa attatgagca tgactatgag ggagcagacg atatgccttc
     1261 tcatatcaaa tcctcaatgc tgggaacatc gcttgtattg ccggtacata aagggcgtat
     1321 tcagaccggc acctggcaag gcatctggct gggggaacat cgcatccacg gcggatcgcg
     1381 tcgcatcatc gcgacactac aaggggagta aaaaatgacc atttcggagt tgctacaata
     1441 ttgcatggca aaaccaggcg cagaacagag cgtgcataac gactggaaag cgacgcagat
     1501 caaagtggaa gatgtactgt ttgcgatggt gaaagaagta gaaaatcgcc cagctgtttc
     1561 gctgaaaacc agcccggagc tggcggagct gctacgtcag cagcacagcg atgtgcgtcc
     1621 tagccgccat ctgaataaag cgcactggag caccgtgtat ctcgacggtt cgctgccgga
     1681 ttcgcaaatc tattatctgg tggatgcgtc ttatcagcag gcagtgaatt tactaccgga
     1741 agaaaagcgt aaattgctgg tacaactctg aaaggaaaag gccgctcaga aagcggcctt
     1801 aacgattaca gcatcggctt gaggaagcgt gccgtatgcg aagcttcgca ctccgcgacg
     1861 gtttctggcg taccggagac gaggatttcg ccgccgccac tgccgccttc tggtcccagg
     1921 tcgacaatcc agtcagcggt tttgatcacg tcgagattgt gctcaatcac cacaatggta
     1981 ttgccctgat cgcgcagttt atgcagcacg tcgaggagtt gctgaatatc ggcgaagtgc
     2041 agaccggtgg tcggctcgtc gagaatatac agcgtctgcc cggtgccgcg ttttgacagc
     2101 tcacgcgcca gcttcacgcg ctgggcttca ccaccagaaa gtgtggttgc ggactgcccc
     2161 aggcgaatgt acgtcagacc aacgtccatc aacgtttgca gcttacgcgc cagagctggc
     2221 accgcatcaa agaactcacg cgcctcttcg atggtcatat ccagcacttc gtggatggtt
     2281 ttgcctttgt acttaatttc cagcgtttca cggttatagc gtttaccttt gcactgatcg
     2341 cacggcacgt agatatccgg caggaagtgc atctccactt tgatcacacc gtcgccctga
     2401 caggcttcgc agcgcccgcc acggacgtta aagctgaaac gtcctggcgt ataaccacgc
     2461 gcacgggatt ccggtacgcc cgcaaacagt tcgcgcacag gtgtaaacac gccggtatag
     2521 gtcgccgggt tagaacgtgg agtacgacca attgggcttt ggtcgatatc gatcacttta
     2581 tcgaaatgct ccagcccctg aatatcgcga tacggtgccg gttcggcgat ggtcgcacca
     2641 ttcaactggc gttgggcaat cgggaacagt gtgtcgttaa tcagcgtcga tttaccggaa
     2701 cctgaaaccc ctgtgatgca ggtaaacaga ccgactggca gcgtgagcgt cacgtccttc
     2761 aggttgttgc cgcgtgcgcc tgtcagcttc agcacttttt ccggatttgc cggaacgcgt
     2821 ttcttcggca cttcaatctt gcgtttaccg ctcatgtact gcccggtcaa cgactccggc
     2881 accgccataa tcgcttccag cggaccttct gcgaccactt caccgccgtg aacacctgcg
     2941 cccgggccaa tgtcgatcac atggtcagcg gcgcgaattg cgtcttcgtc gtgctccacc
     3001 acaatcacgg tattaccgag atcgcgcaga tggataagcg tacccaacag gcgctcgtta
     3061 tcgcgctggt gcaggccgat agatggctcg tccagcacgt acataacgcc aaccaggccc
     3121 gcaccaatct ggctcgccag acggatacgc tgggcttcac cgccggaaag cgtttctgcc
     3181 gagcgggaaa gcgtcaggta attcaggccg acgttaacga ggaatttcaa acgatcgccg
     3241 atctctttaa ggattttttc cgcaatcttc gcccgttgac cagcgagttt gagattgttg
     3301 aagaattcca tcgcatgacc gatgctcatg tcggagatag caggcagcgg cgtattctcg
     3361 acataaacgt ggcgcgcttc ccgacgcaga cgcgtccctt cgcagctggc gcatgggcga
     3421 ttgctgataa acttggctaa ttcttcacgt accgcactgg attctgtctc tttataacgg
     3481 cgctccatat tgtgcagcac gccttcgaac ggatgacgac ggatggaggt atcgccacga
     3541 tcgttcatgt atttgaattc aatgttttct ttgccagaac cgtacaacac cactttatgc
     3601 acgttcgcgc tcaggctgcc ccacggcgct tcgacgtcga acttatagtg atctgccagc
     3661 gatttcagca tctggaagta atagaagttg cggcgatccc agccacggat cgcaccgcca
     3721 gccagtgaca gctcggggtt ttggatcacg cggtcaggat cgaaatattg ctgtacgcca
     3781 aggccgtcac aggttgggca ggcacctgcc gggttgttaa acgaaaacag tcgcggctcc
     3841 agttcacgca tactgtagcc gcaaattggg caggcgaagt tggcggagaa cagcagctct
     3901 tccgctttcg ggtcgtccat atccgccact accgctgtac caccggaaag ctccagcgcg
     3961 gtttcaaacg actccgcaag acgttgggta agatcgtcac gcaccttgaa gcgatcaacc
     4021 accacttcaa tggtatgttt cttttgcagt tccagtttcg gcggatcgga aagatcgcag
     4081 acttcgccat caatacgagc acggatgtaa ccctggcttg ccaggttctc cagcgttttg
     4141 gtgtgttcgc ctttgcgctc tttaatgatt ggcgcgagca gcatcagacg cttgccttcc
     4201 ggctgcgaca gcacgttatc caccatctgg ctgacggttt gcgccgccag cgggacgtcg
     4261 tggtccggac agcgcggttc gccgacgcgg gcgaacaaca aacgcaaata gtcgtggatt
     4321 tcggtgattg tccccaccgt agaacgcggg ttatgagacg tcgatttctg ctcaattgag
     4381 atggcaggag aaagcccctc aatatgatcg acgtccggct tttccatcag tgacagaaac
     4441 tgccgcgcgt aggcggaaag ggattcaacg taacggcgct gcccttcggc atataaggtg
     4501 tcgaaagcga gcgaggattt gccagaaccc gaaagcccgg tcacgacaat gagcttgtcg
     4561 cgggggataa cgaggttgat gtttttgaga ttatgggtgc gggcgccccg aacttcgatc
     4621 ttatccattc acctttcccg gattaaacgc ttttttgccc ggtggcatgg tgctaccggc
     4681 gatcacaaac ggttaattat gacacaaatt gacctgaatg aatatacagt attggaatgc
     4741 attacccgga gtgttgtgta acaatgtctg gccaggtttg tttcccggaa ccgaggtcac
     4801 aacatagtaa aagcgctatt ggtaatggta caatcgcgcg tttacactta ttcagaacga
     4861 tttttttcag gagacacgaa catggccagc agaggcgtaa acaaggttat tctcgttggt
     4921 aatctgggtc aggacccgga agtacgctac atgccaaatg gtggcgcagt tgccaacatt
     4981 acgctggcta cttccgaatc ctggcgtgat aaagcgaccg gcgagatgaa agaacagact
     5041 gaatggcacc gcgttgtgct gttcggcaaa ctggcagaag tggccagcga atatttgcgt
     5101 aaaggttctc aggtttatat cgaaggtcag ctgcgtaccc gtaaatggac cgatcaatcc
     5161 ggtcaggatc gctacaccac agaagtcgtg gtgaacgttg gcggcaccat gcagatgctg
     5221 ggtggtcgtc agggtggtgg cgctccggca ggtggtaata tcggtggtgg tcagccgcag
     5281 ggcggttggg gtcagcctca gcagccgcag ggtggcaatc agttcagcgg cggcgcgcag
     5341 tctcgcccgc agcagtccgc tccggcagcg ccgtctaacg agccgccgat ggactttgat
     5401 gatgacattc cgttctgatt tgtcattaaa acaataggtt atattgtttt aaggtggatg
     5461 attaaagcat ctgccagcca taaaaaagaa gcctccgtta tggaggcttc tacgtatcag
     5521 gtcaaaatca ttggccattg tggcggtgtc tgattcatct cttctatcat caccgactcg
     5581 atgttgtgcc agatagcggt gatgtccgcc attgtgatgc caagcaaccc cattgcgaac
     5641 caacaggcgg aaacaacgcc cagaccgctg ctgatcaccg caagcccgac ataatcagac
     5701 cgacgaaaac ggatgttgag tgtgctggcc agaaacatca ttacggcact aagaagttgc
     5761 cagcgaagaa gaaccacgcc agtggtgagg gtagccatca aacaattcct ctgaaaagag
     5821 ccgatgccct ggacagcgcg gcgttgttca cgggaggcgt ggtacactct ggctatcgcg
     5881 gggcttgcag aacacaaaaa tgaaacactc tgtttgtttc attaattttg tgaactatat
     5941 cacaattgat tgtttgttag ccatattagg ccgtgacttt tattgctgta cagattatgt
     6001 ggtttttcag tggcattaag ggcatatctt cccgccgcct ctgcattcct gtaggaaatt
     6061 aattttgaat atcaatgaat tattttcatc caggtgacga ttagaaaggt atcaatttca
     6121 aatcaggcaa aagtgctatt tataccgtaa gatttatcta aagacgtcgg tacctagggt
     6181 tttcaccttg caatggccgg gtataaacag gcaggaaatt gatagcaatg agtcatcgtg
     6241 cacgacacca attactggcg ttgccgggca ttatcttttt agttctcttt cccatcattc
     6301 tttcgctatg gattgccttc ctttgggcaa aatcagaagt gaataatcag ctccgaacct
     6361 ttgctcaact ggcactggat aaatccgagc tggtcattcg ccaggcagat ttagtgagcg
     6421 atgcagctga acgctatcag gggcaagttt gcactccagc ccatcaaaag cgaatgttga
     6481 atattattcg tggctatctt tatattaatg aattgatcta tgcccgtgat aaccattttt
     6541 tatgctcatc gctgatagcg cctgtaaacg gctatacgat tgcaccggcc gattataagc
     6601 gtgaacctaa cgtttctatc tattattacc gcgatacgcc ttttttctct ggctataaaa
     6661 tgacctatat gcagcgggga aattatgtgg cggttatcaa ccctctcttc tggagtgaag
     6721 tgatgtctga tgacccgaca ttgcaatggg gtgtgtatga tacggtgacg aaaacctttt
     6781 tctcgttaag caaagaggcc tcggcagcaa cgttttctcc gctgattcat ttgaaggatt
     6841 taaccgtaca aagaaatggc tatttatatg cgacagttta ttcgacaaaa cgcccaattg
     6901 cagccattgt tgcgacttca tatcaacgtc ttataaccca tttttataat catcttattt
     6961 ttgcgttgcc cgccggtatt ttggggagtc ttgttctgct attactctgg ctacgtattc
     7021 gacaaaacta tttatctccc aaacgtaaat tgcaacgcgc cctcgaaaaa catcaacttt
     7081 gtctttatta ccagccaata atcgatatca aaacagaaaa atgtatcggc gctgaagcgt
     7141 tgttacgttg gcttggtgag caggggcaaa taatgaatcc ggcagagttt attccgctgg
     7201 cagaaaagga ggggatgata gaacagataa ctgattatgt tattgataat gtcttccgcg
     7261 atctgggcga ttacctggca acacatgcag atcgctatgt ttctattaac ctgtcggcct
     7321 ccgattttca tacgtcacgg ttgatagcgc gaatcaatca gaaaacagag caatacgcgg
     7381 tgcgtccgca gcaaattaaa tttgaagtga ctgaacatgc atttcttgat gttgacaaaa
     7441 tgacgccgat tattctggct ttccgccagg caggttacga agtggcaatt gatgattttg
     7501 gtattggcta ctctaacttg cataacctta aatcattgaa tgtcgatatt ttgaaaatcg
     7561 ataaatcgtt tgttgaaacg ctgaccaccc acaaaaccag tcatttgatt gcggaacaca
     7621 tcatcgagct ggcgcacagc ctggggttaa aaacgatcgc tgaaggcgtc gaaactgagg
     7681 agcaggttaa ctggctgcgc aaacgcggcg tgcgctattg ccagggatgg ttttttgcga
     7741 aggcgatgcc gccgcaggtg tttatgcaat ggatggagca attacccgcg cgggagttaa
     7801 cgcgcgggca ataaaactac aggcggtggc gataatcgct gggagtgcga tcaaactgcc
     7861 gacggaaaac gcgggagaag gtctgctgcg agacataacc caggtccatt gcgatatcaa
     7921 aaatcggacg ctcggtggtg cgcaactcaa cggcggccag taacaggcga cgttggcgaa
     7981 tgtaatcgcc aagcgtctga tgcgtcaccg tgcggaacat tcgttgcaag taccactttg
     8041 aatagcctga tttttttgcg actacatcaa tgttaagcgg ctggtcaata tgctcgtcaa
     8101 tccatgcgat aagatcctga ataattttct gatgggacat aaatctgcct cttttcagtg
     8161 ttcagttcgt taattcatct gttggggagt ataattcctc aagttaactt gaggtaaagc
     8221 gatttatgga aaagaaatta ccccgcatta aagcgctgct aacccccggc gaagtggcga
     8281 aacgcagcgg tgtggcggta tcggcgctgc atttctatga aagtaaaggg ttgattacca
     8341 gtatccgtaa cagcggcaat cagcggcgat ataaacgtga tgtgttgcga tatgttgcaa
     8401 ttatcaaaat tgctcagcgt attggcattc cgctggcgac cattggtgaa gcgtttggcg
     8461 tgttgcccga agggcatacg ttaagtgcga aagagtggaa acagctttcg tcccaatggc
     8521 gagaagagtt ggatcggcgc attcatacct tagtggcgct gcgtgatgaa ctggacggat
     8581 gtattggttg tggctgcctt tcgcgcagtg attgcccgtt gcgtaacccg ggcgaccgct
     8641 taggagaaga aggtaccggc gcacgcttgc tggaagatga acaaaactaa agcgccacaa
     8701 gggcgcttta gtttgttttc cggtctttgt ctttctctct atcccgctgg tacacaggag
     8761 ggtttccccc gacgtcaaca cacctcattc gagcacgtgg tggaggttcc ggttggtgtt
     8821 gatgctttaa ttgtatgtca ccgacgtttc ttcgccagtg taaaagtata ctttttaacc
     8881 gcaatatttt tgtcatctca gacgattttt tatcgcaatc ctgaacggta tacggctcga
     8941 taacgctgca atcttgcgca ccgacgataa cgtttgcgca tcaattgcct gttttttcat
     9001 cgtcaagaca ataaaagaga aaaaagcagc aaacttcggt tgaaaaagcc gctatgatcg
     9061 ccggataatc gtttgctttt tttaccaccc gttttgtatg cgcggagcta aacgtttgct
     9121 tttttgcgac gcagcaaatt gtcgcaaacc tggagcagga agataacgtt tcgctggcag
     9181 gggattgtcc gccacgcatc ttgacgaaaa ttaaactctc aggggatgtt ttcttatgtc
     9241 tacgccatca gcgcgtaccg gcggttcact cgacgcctgg tttaaaattt cacaacgtgg
     9301 aagcactgtc cgtcaggaag tggttgccgg gttaacaacg tttctggcga tggtctactc
     9361 ggtcatcgtc gttccaggta tgttgggtaa agcaggcttc ccgcctgcgg cagttttcgt
     9421 tgcaacctgt ctggttgccg gactcggttc tatcgtgatg ggtctgtggg ctaatctgcc
     9481 gttggcgatt ggttgcgcca tctccctgac ggcgtttacc gcattcagcc tggtgctggg
     9541 gcaacatatt agcgtacctg tcgcgctggg tgccgtgttc ctgatgggtg tgctgtttac
     9601 ggtaatttct gccacgggta tccgtagctg gattttgcgc aacttgcctc acggtgtggc
     9661 gcacggcacg gggatcggta tcggtctgtt cctgctgctc attgccgcta atggtgtcgg
     9721 tctggtgatt aaaaacccgc ttgatggtct gcccgttgcg ctgggtgatt tcgcgacctt
     9781 cccggtgatt atgtcactgg taggtctggc ggtgatcatc ggcctggaaa aactgaaagt
     9841 ccctggtggc attctgctga ccattatcgg tatctcaatt gtcggtttga tcttcgatcc
     9901 taacgtccat ttctccggcg ttttcgccat gccttcattg agcgatgaaa acggcaattc
     9961 actgattggc agcctggaca ttatgggcgc gctgaatcct gtagtcctgc caagcgttct
    10021 ggcgctggtg atgacggcag tatttgatgc caccggaact atccgtgccg tcgccggcca
    10081 ggcgaacctg ctggataaag atgggcagat catcgacggt gggaaagcac tgaccactga
    10141 ctccatgagc agcgttttct ctggcctggt gggtgctgct ccggcagcgg tatacatcga
    10201 gtctgcggcg ggtacggcgg cgggcggtaa aaccggtttg acggctatca ccgttggcgt
    10261 gctgttcctg ctgattctgt tcctctctcc gctctcttac ctcgttccgg ggtatgcaac
    10321 ggctccggcg ctgatgtacg ttggcctgct gatgctgagc aacgtggcga aaatcgactt
    10381 tgctgatttt gttgatgcga tggcgggtct ggttacggcg gtattcatcg tgctgacctg
    10441 taacatcgta acaggcatca tgatcggctt cgcgactctg gtgattggtc gtctggtttc
    10501 cggcgaatgg cgcaagttga acatcggtac ggtcgttatc gccgtggcgc tggtgacctt
    10561 ctatgcgggt ggctgggcta tctaatcttt catcggattc tgaaaacggg tggcaatggc
    10621 tgcccgtttt tattttctcc gcacattgtg tgagcttttg cgatattctg aaaaaatgag
    10681 aattcaggca taacgtcttt ctcagatcat aaaaaaacat cgcaaacagg gaacgcatgg
    10741 aaatcttctt caccatactg ataatgaccc tcgtggtctc gctgtccggg gtggtcactc
    10801 gtgtcatgcc ctttcagatc ccgcttccgc ttatgcaaat cgccatcggt gcgctactgg
    10861 cgtggccgac gtttggtttg catgtggagt ttgatcctga actcttttta gtcttgttta
    10921 tcccgccgtt gctgttcgct gatggctgga aaacgccgac ccgtgaattt ctcgaacatg
    10981 gtcgagagat ttttggcctc gcgctggcgc tggtggtggt caccgtggtc ggcattggct
    11041 tccttattta ctgggtggtg ccgggcattc cgctgatccc cgcctttgcg ctggcggcag
    11101 tgctttctcc gaccgatgct gtggcgctct ccgggattgt tggcgaaggg cgcatcccga
    11161 aaaaaatcat gggtattttg cagggcgaag cgttgatgaa tgacgcctcc ggcctggtgt
    11221 cgttgaagtt tgccgtggca gtggcgatgg ggacgatgat cttcaccgtt ggcggtgcga
    11281 cggtcgaatt tatgaaagta gccattggcg gtattctcgc cggctttgtg gtgagctggc
    11341 tgtatggtcg ctcgctgcga ttcctcagcc gctggggcgg tgatgaaccc gcgacgcaga
    11401 tcgttctgct gttcttgctg ccattcgctt cttatctgat tgccgaacat attggcgttt
    11461 ccggcatcct cgctgcggtt gccgccggga tgaccatcac ccgctccggt gtgatgcgcc
    11521 gtgcgccgct ggcaatgcgc ctgcgcgcaa acagcacctg ggcgatgctg gaatttgtct
    11581 ttaacggcat ggtgttcctg ctgttaggtc tgcagctgcc gggtattctg gagacgtcgc
    11641 tgatggcggc agaaatcgac cctaacgtcg aaatctggat gctgtttacc gatattattc
    11701 tgatatatgc ggcgctgatg ctggtccgtt tcggctggct gtggacgatg aaaaagttca
    11761 gcaaccgctt cctgaagaag aagccgatgg agtttggttc gtggaccaca cgagaaatcc
    11821 tgatcgcgtc tttcgccggg gtgcgtgggg cgatcactct ggccggtgtg ctctctatcc
    11881 cgctgctctt gccggatggt aacgtcttcc cggcgcgcta tgagctggtg ttcctggcgg
    11941 ctggcgtcat tctcttctcg ctgtttgtcg gcgtggtgat gttgcctatt ctgctacaac
    12001 acattgaagt cgcggaccat tcgcaacaat tgaaagaaga acgtattgcg cgagcggcaa
    12061 cggcagaagt ggcgattgtg gcgatccaga aaatggagga gcgtctggcg gcggataccg
    12121 aagagaatat cgataaccag ctgcttacgg aggtcagttc tcgcgtcatt ggtaacctgc
    12181 gtcgtcgcgc cgatggacgt aacgacgttg aaagttccgt gcaggaagag aaccttgagc
    12241 gtcgcttccg tctggcggca ttgcgttctg aacgtgctga actgtaccac ctgcgcgcca
    12301 cgcgggagat cagcaacgaa acgctgcaaa aattactgca cgatctcgat ttgcttgaag
    12361 cgttgctaat tgaggaaaat cagtaagccg aaagcgacgt aaccaggccc gaggtaatct
    12421 gaaaaaggtg caatcttttg cacctttaac aggttagctc gggctaacat tcatgattct
    12481 aaaacaaata agttatctct cttgtaaagt caattcattg gctgttcttc gacttaacct
    12541 caatctgcgt gttgcataat atctgcagta atcttcatag cgactgcatt aatattatcc
    12601 tcagtttgtg acatgccagt atgagaggta caatcttctt caaagaattt ctgtagtgtt
    12661 accagggtat cactatcaat ggggatattt tcctccccac ggagaataga aatgcccatt
    12721 ccctggacgc tagcctcatc aaaattaacc cgatagagac gggtattaaa catacaggct
    12781 tttgttaaaa tagaacagtt gaaatggcag ttgcttaaat tggcattatt aaaattaaca
    12841 ttagccatat tagagccggt acatataacc atttcgagga atgtatttgt tagccacgta
    12901 caaaagagtt tgctatcaat taagtttata ttctggagga ttgctttgtt cagattgacg
    12961 cgagacagat cagcatgttc aaagttaatt aaatcgagtt tagagaaact aaggtcggct
    13021 ttaaataaat taacatatgg cgctattact ttataaagat ttacctcagc catgtacgcc
    13081 atataaaaat ttgaatagtc aagaaaggta tttttaatat tggctctatt cattgctgca
    13141 ttgtagaatt gaacatgcat agcggagcat gcatttagag ttccgtcggc catttttatc
    13201 tgagcgaaat ttgagtcatc gagaatacaa ttatcaaaaa gtattttttc cattatggcg
    13261 taataaaaat tcgttttata catattacag tcctggaaaa tagcgttttg tagtcagcaa
    13321 cctgcaaaat tcgaattgga taaatcttca tctttaatca cgataccttt caggctaagg
    13381 tgtgaaaaat cgtaaccacc atttactgtc tgtggaattt cgatagtatt attatggacg
    13441 gcgacattat ttataaaata taccacctta atggatactt ccttttcttt actaaggtaa
    13501 actcttctcg taattctatc gtcacattta ttatttgcat ataaagctat atctataaaa
    13561 tgtttatgta ttttcctgtc cgtttccttc gtgatttttc ctgatggtaa actatcataa
    13621 attcttttca ttttctgaat caaacctatc actttttcat gatcatccat cttgaaaaga
    13681 gagagggctt tattgtcaat taacgtgttt ttatgatgtt tgctgtaatt atatgatttt
    13741 gttttctttg tatgtgatga acttgtgaaa gagtggttat cgttaatctg gcaaagagag
    13801 aaaaacatat tatttaaacc attatagcgc ataaaatatc cttataat
//
LOCUS       DAZGAN020000124        13836 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGAN020000124 DAZGAN020000000
VERSION     DAZGAN020000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.124"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(120..872)
                     /gene="tcyN"
                     /locus_tag="ACHKYO_RS20370"
                     /old_locus_tag="pgaptmp_004074"
     CDS             complement(120..872)
                     /gene="tcyN"
                     /locus_tag="ACHKYO_RS20370"
                     /old_locus_tag="pgaptmp_004074"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HGU4430835.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(869..1537)
                     /gene="tcyL"
                     /locus_tag="ACHKYO_RS20375"
                     /old_locus_tag="pgaptmp_004075"
     CDS             complement(869..1537)
                     /gene="tcyL"
                     /locus_tag="ACHKYO_RS20375"
                     /old_locus_tag="pgaptmp_004075"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HGU4430836.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1552..2538)
                     /gene="dcyD"
                     /locus_tag="ACHKYO_RS20380"
                     /old_locus_tag="pgaptmp_004076"
     CDS             complement(1552..2538)
                     /gene="dcyD"
                     /locus_tag="ACHKYO_RS20380"
                     /old_locus_tag="pgaptmp_004076"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HGU4430837.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2643..3443)
                     /gene="tcyJ"
                     /locus_tag="ACHKYO_RS20385"
                     /old_locus_tag="pgaptmp_004077"
     CDS             complement(2643..3443)
                     /gene="tcyJ"
                     /locus_tag="ACHKYO_RS20385"
                     /old_locus_tag="pgaptmp_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4430838.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3531..4082)
                     /gene="fliZ"
                     /locus_tag="ACHKYO_RS20390"
                     /old_locus_tag="pgaptmp_004078"
     CDS             complement(3531..4082)
                     /gene="fliZ"
                     /locus_tag="ACHKYO_RS20390"
                     /old_locus_tag="pgaptmp_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HGU4430839.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4128..4847)
                     /gene="fliA"
                     /locus_tag="ACHKYO_RS20395"
                     /old_locus_tag="pgaptmp_004079"
     CDS             complement(4128..4847)
                     /gene="fliA"
                     /locus_tag="ACHKYO_RS20395"
                     /old_locus_tag="pgaptmp_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HGU4430840.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5168..6820)
                     /gene="fliC"
                     /locus_tag="ACHKYO_RS20400"
                     /old_locus_tag="pgaptmp_004080"
     CDS             complement(5168..6820)
                     /gene="fliC"
                     /locus_tag="ACHKYO_RS20400"
                     /old_locus_tag="pgaptmp_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HGU4430841.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITSSNGDKLYIDTTGRLTKNGFSASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPNTITYSVTGAKVDQAAFDKAVSTSGNDVDFTTAGY
                     SVDGATGAVTKGVAPVYIDNNGALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            7086..8492
                     /gene="fliD"
                     /locus_tag="ACHKYO_RS20405"
                     /old_locus_tag="pgaptmp_004081"
     CDS             7086..8492
                     /gene="fliD"
                     /locus_tag="ACHKYO_RS20405"
                     /old_locus_tag="pgaptmp_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGU4430842.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTIS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8517..8927
                     /gene="fliS"
                     /locus_tag="ACHKYO_RS20410"
                     /old_locus_tag="pgaptmp_004082"
     CDS             8517..8927
                     /gene="fliS"
                     /locus_tag="ACHKYO_RS20410"
                     /old_locus_tag="pgaptmp_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGU4430843.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8927..9292
                     /gene="fliT"
                     /locus_tag="ACHKYO_RS20415"
                     /old_locus_tag="pgaptmp_004083"
     CDS             8927..9292
                     /gene="fliT"
                     /locus_tag="ACHKYO_RS20415"
                     /old_locus_tag="pgaptmp_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HGU4430844.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEINPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFS"
     gene            9370..10857
                     /gene="amyA"
                     /locus_tag="ACHKYO_RS20420"
                     /old_locus_tag="pgaptmp_004084"
     CDS             9370..10857
                     /gene="amyA"
                     /locus_tag="ACHKYO_RS20420"
                     /old_locus_tag="pgaptmp_004084"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU4430845.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSKFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10891..11304)
                     /gene="yedD"
                     /locus_tag="ACHKYO_RS20425"
                     /old_locus_tag="pgaptmp_004085"
     CDS             complement(10891..11304)
                     /gene="yedD"
                     /locus_tag="ACHKYO_RS20425"
                     /old_locus_tag="pgaptmp_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HGU4430846.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11491..12696
                     /gene="yedE"
                     /locus_tag="ACHKYO_RS20430"
                     /old_locus_tag="pgaptmp_004086"
     CDS             11491..12696
                     /gene="yedE"
                     /locus_tag="ACHKYO_RS20430"
                     /old_locus_tag="pgaptmp_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HGU4430847.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12693..12926
                     /gene="yedF"
                     /locus_tag="ACHKYO_RS20435"
                     /old_locus_tag="pgaptmp_004087"
     CDS             12693..12926
                     /gene="yedF"
                     /locus_tag="ACHKYO_RS20435"
                     /old_locus_tag="pgaptmp_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HGU4430848.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13035..13706
                     /gene="yedK"
                     /locus_tag="ACHKYO_RS20440"
                     /old_locus_tag="pgaptmp_004088"
     CDS             13035..13706
                     /gene="yedK"
                     /locus_tag="ACHKYO_RS20440"
                     /old_locus_tag="pgaptmp_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HGU4430849.1"
                     /translation="MCGRFAQSQTRVDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(13746..>13836)
                     /locus_tag="ACHKYO_RS20445"
                     /old_locus_tag="pgaptmp_004089"
     CDS             complement(13746..>13836)
                     /locus_tag="ACHKYO_RS20445"
                     /old_locus_tag="pgaptmp_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430850.1"
                     /translation="GHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         3360 a         3596 c         3463 g         3417 t
ORIGIN      
        1 gcaagccatc gcattctctt atgttattaa tgagttatgc tgatttgtta agcagtttta
       61 tcacgcttga aatggcgtcc cgccccgaca ggtgaatcgt cggggctgat tttttcttat
      121 tattgcagca gaaacttctc gaggaactgg cgggtgcgag gctgctgggg atcagcaaat
      181 aacgcttttg ctgccccctg ctcgactatc cgcccctggt ccataaaaat cgcccggtcc
      241 gcaacatccc gggcaaagct catttcgtgc gtcacaatca ccatcgtgcg cttttcctgc
      301 gccagctggc ggattgtgtt caggacttcg cccaccagct ctggatccag cgctgacgtt
      361 ggctcgtcaa acaaaatcac ctcaggacgc attgccagcg cacgcgcaat cgcaacacgc
      421 tgctgttgac cgccagacaa acgacgtgga tagctggttt ctttaccggc cagcccaact
      481 ttcgccagca gctcgcgagc gcgcgccgtg gcctcttctt tcggttcgcc tttgacgatc
      541 accggccctt caataatgtt ttccagcacc gtacgatgcg gaaacaaatt aaagttctgg
      601 aagacaaacc cgacgtgctg acgcaactgg cgaatcagcg atttttgctg acttaatgaa
      661 cgtgcagtat caatagtgat atcgccaacg gtgatcgttc ccgcttccgg ttgttccagc
      721 agatttatgc tgcgtagcaa cgtggttttg ccggaaccac tcggaccgat aattgccacc
      781 acttcgccag gctttacctc gaggtcgata ccgtgcagca ccgtctgacc gtgaaatttt
      841 ttcaccaggt tcttaacttc aatggcactc attttggttc tctctcctgg cgattaagtt
      901 gattctcaaa atggttctgc aacgtcgata atactgtcgc catgatccag tagatgagcg
      961 aagccgccag gtacatggtg aacacctcca gcgtgcgcga ggtaatcaac tgcgcttgac
     1021 ggaacagctc cggcacctgg attgtcgcgg ccagcgacgt atcttttacc aggctgataa
     1081 agctgttcga cagcggcggc aacgccaccc gcgccgcctg tggcaaaatc gcgcgacgca
     1141 tggtctgcca cggcgtcata ccaatactgg cagcggcttc ccactgacct ttatcaatag
     1201 aagaaatggc ggcgcgcagc gtttcggcag cataggcggc agtgttcagc gacagaccaa
     1261 tcattgctga cggaatggga tctaattcga taccaaactg cggcaggccg taatagatca
     1321 taaacagttg tgcgatgagc ggtgtaccac gaaaaataga gatataaaaa cgcgccagcc
     1381 agcgcacggg ccagattggc gacagacgca tcagcgcgag aataaaccct agcagtaagc
     1441 caaaaaacat gccgccgata ctgagttgca gggtataccc agcccctttc aacaggaacg
     1501 gcaaggaatc aataaccagt tgtatacttt cttgcatgag cgttcccgga gctaaacgtg
     1561 gggatgatag gcgaacagcg caggcgcgcc gccggtatga ataaacagaa tcggcccttc
     1621 atctttgaag cgtttctgac tgataccgtc aatcagcccc gccatcgctt ttccggtata
     1681 cacaggatca agcagaatgc cttccagacg cgccagcaat ttcactgctt ccatgccttc
     1741 gtcgttcggc acgccgtagc caggtgcaaa atagtcatcc cagagtaaaa tttccgctga
     1801 tgcggtcagc tccagttctt tcgcaatcgc ctgttgtagg ttaaccactt tcggcaattg
     1861 atcggcaacg gaacgcgaca cggtcacgcc aatcagttcg ctttcaggca tcaggtgttc
     1921 cagcccaaca gccaatccgg cgtgagttcc ggcactgccc gatgcgacca ccaccgacga
     1981 aatattaacc gccccttcac actgttgcgc gatttccagc gcactctcca cataacccag
     2041 cgcgcccaga gcattagaac cgccaaccgg aatgacatac gggcgaaagc cttgtgcttc
     2101 gactcgcgtc gccagctctt ccagttgggc attgggatcg gtcagtgcgt cgcacatttc
     2161 aatctgggta ttgaacagat ccagcaacaa acgattgccg ttggttaaat agttttctgc
     2221 ggttgtgcca ataggatttt ccagcagcgc cacgcagtgc agaccgagtt tcgccgcgac
     2281 tgcggcagtc tggcgcacat ggttagactg gatcgcgccg gcagtaatca gcgtatcggc
     2341 accttcgcgc agggcatctg ccgcgagaaa ttccagctta cgtaatttat tgccgcccat
     2401 tgccatgggt gtgacgtcat cccgtttgat gaaaatttcc cgtccaagat aatcagaaaa
     2461 gcgcggcaga tattcgagcg gcgttggcgc gccgataaac tccaaccgtg gaaaacgggt
     2521 taaattatgc agtggcataa cagcctccga tgtgtgttgt tgtgattttc ttattatgca
     2581 cgctgaaaac gcgtaaataa aaaaggcgct agtgaaagcg cccttttttg tcattatgct
     2641 gattatttgg tcacatcagc accaaaccat ttttcggaaa gagcttgcag agtgccgtct
     2701 ttttgcattt ccgcaattgc atcattcacc gctttcagca ggtcttcatt tcctttacgc
     2761 agcgccacgc cagactcctg acgggagaat gcttcaccgg ttactgccag cgtatcgttg
     2821 gttttcttca ccagatccag cgccgccaga cgatcaacga ggatcgcatc gatacggcct
     2881 acgcgcagat cctgatattt ggtcgggtca tcatcatagg tacgcacatc gacgccctga
     2941 acattctgcc gcagccactc ttcatagttg gtgcccagac cgacacccac ttttttgcct
     3001 ttcagatcag cagccgtttt aatggtgcct tcgttacctt ttttcaccag cgcctgaata
     3061 ccggaaatgg tgtaaggggt agagaaatcg tattttttct tgcgctcgtc agaaatggtg
     3121 acctgattaa tcaccacatc aatgcgttta gagtccagcg acgccagcat accgtcccat
     3181 ttggtcggtt ttagtgacgc ctcaacgcca agatgttttg ccagctgttg ggcaaattcc
     3241 acttcaaaac cggttaattt gccgtcatct ccctgaaaac tgaacggcgg ataagtgcct
     3301 tccagcccta ccagcagcgt gccgcgctct ttaactttat taagcagacc ttcatctgca
     3361 aaacttttaa cgctcatgcc cgcaaccagc gctacggcca tcacacccat caatgcctga
     3421 cgtcccagat gtgctaattt catatttacc ccgaatgttg ttatgtctgt ttgcagtgta
     3481 gagccattgt ttgtaaacac aaaaacaact ccgctacatc ttattcttat ttaatatata
     3541 tcagaagcag gcaggctgga ggatttctgc acaagtccgg tacacgtttg acgctgatag
     3601 tgttgatact tccgtaatgc gatgcggtaa ttgttggtgc tggtggtggg cagccacggg
     3661 gcgaggattt catcaaggaa accgtcctgc agcggatcga gggaaatatt ttgctcgtgt
     3721 agatgattcc ccaaacggcg cagacgaacg acatattcac gcaccgtacc agggctcatt
     3781 tcagtttgct caaaaagaaa ttgcttaaag ccgataatat cgaaaaaatc actctgcgtt
     3841 ttgcaatgta aatcaccgca gaatcggcac aatgccgccc atgatttttg ttccacttgc
     3901 cagccatttt catcaagcag catgtccagg cgggaaatct cgattttatt cactattttg
     3961 ccgtcgcgaa cgagggtaat gcggtcgagt aatttacggc aatgcgcgca atgggtctgg
     4021 ctgtgtttaa agtctttaag atagcggctt aatggccgtc ttttcaggtg ctgcaccatc
     4081 attaagaact cctggtagtc aaaattaaag tgcggcattt actgacgtta taacttaccc
     4141 agtttagtgc gtaaccgttt aatggcctgg ctgtgtaact gactgacccg cgattccccg
     4201 acctccagca ccgcgccaat ctctttgaga ttcagctctt cctggtaata gagggttaat
     4261 accagttttt cgcgctccgg caacgtttcg atagcttcca tcactcgctg gcgcagatta
     4321 ctgtccagca gttgttgtag cgggttttct cgctgatgat catcagtaac cagttcgatg
     4381 ctatcgccgt gctcttcgcg ccactcatcg taggagaaga gctggctgtt attggtgtcg
     4441 agcaacattt ggcgataatc ggcaatatcg atccctaaac gttccgctac ctcagtttcc
     4501 gtggcgttgc ggccaagttc ctgctccagt tgccctattg cctgtgccac ttcacgcgcg
     4561 ttgcgtcgca cgctgcgcgg cacccagtca cggctgcgaa gttcatccag catagcgcca
     4621 cggatacgct gcactgcgta agttgtaaat gccgttcctt gtagggcgtc atagcgttcg
     4681 acggcattaa gtaacccaat gccgcccgcc tgtagcagat cgtcaagttc cacgctcgcg
     4741 ggcagtcgaa cctgcaggcg caatgcttcg tgacgcacca gcgggacata acgctgccac
     4801 agcgagtgtt tatccattac accttcagcg gtatagagtg aattcacgat aaacagccct
     4861 gcgttatatg agttatcggc atgattatcc gtttctgcag ggtttttaat cggacgatta
     4921 gtgggtgaaa tgaggggtta tttgggggtt accggtaaat tgcggacaga aaaaaacccc
     4981 gccggtggcg gggaagcacg ttgctgacaa attaccattc atgttgccgg atgcggcgta
     5041 aacgccttat ccggcctaca aaaatgtgca aattcaataa attgcagttc accttgtagg
     5101 cctgataagc gcagcgcatc aggcaatttg gcgttgccgt cagtctcagt taatcaggtt
     5161 acgacgatta accctgcagc agagacagaa cctgctgcgg tacctggtta gcttttgcca
     5221 gcacggagtt accggcctgc tggatgatct gcgctttcga catgttggac acttcggtcg
     5281 catagtcggc gtcctgaata cgggactgcg cttcggacag gttggtagtg gtgttgttca
     5341 ggttggtgac cgcggaatcc agacggtttt gcaccgcacc gagggaggag cggaatttgt
     5401 cgatggagct gatggcttcg tccagagctt tcagtggatc ggcggtggtt gcagctttag
     5461 tttcagcatc tgtcgtcaat ttaccgtcag catctttata aactgcctta ccgctgccgt
     5521 tagtcactga accatcatcc tgtagataaa aatctacagt atcagatgtg gtcaacgccc
     5581 cgttgttatc aatataaacc ggagcaacac cttttgttac agcgccagtt gcgccgtcga
     5641 cgctataacc tgcggtagtg aaatcaacat cgtttccaga ggttgataca gctttatcga
     5701 aagctgcctg atcaactttt gcacctgtta ctgaataagt aatagtgttc ggcgtagtac
     5761 tattaccggt aaaggagata gaggtaccgc caatgtcaat ggttgtcgct ttggtattat
     5821 tggctgcaag tgtggacaga ctagcctcag tcaaagaagc actaaagccg tttttcgtta
     5881 agcgcccagt tgtatcaatg taaagtttat ctccattgct tgacgtaatt ttaccatcac
     5941 tgctgatctg aacatcctgt gcagaaccat cgatagtaat agtgccctta gcggtatcac
     6001 caacgcccgg ggtcaaatat ttctgaacat cggcagctga agccgttgtg gtatcaaaag
     6061 agtaagactt acttgcactg tcatacttgt aattcgttgc actagccgcc gttccgaagc
     6121 cgttattaat ggttgcctga acagtatcac catcactaac tccagccagc aaatcagacg
     6181 ctttagcagc atcgtaaccc gcactcacag tatatttgtt gcctaccaca gttgcattag
     6241 cagctaccaa gtcagcttta gatgcagcag tgttagcaac agccccgcca ccattcacat
     6301 taaacccact cagccccagc gtatctgagt caattttctt cagatcaatc gtgatagtct
     6361 ggccgtcatt cgcaccaacc tgaattttca tggaaccgtc tttcgccagt acgttcacgc
     6421 cgttgaactg ggtctggcca gatacgcggt caatttcgtc cagacgggat ttgatttcgt
     6481 cctgaatgga gtccagatcc gaatcggagt tagtcccggt agaagcctga accgtcagtt
     6541 cacggatacg ctgtaagttg ttgttgattt cggacagcgc gccttcggtg gtctgcgcaa
     6601 cggaaatacc gtcgttggcg ttacgtgcag cctgagtcag gcctttaatg ttagaagtaa
     6661 aacggttagc aatcgcctga cctgcggcgt catccttcgc gctgttaata cgcaagccag
     6721 aagacagacg ctcgatagaa ctcgacagcg cagactggtt cttgttgata ttattttgag
     6781 tgatcagcga gaggctgttg gtattaatga cttgtgccat gattcgttat cctatattgc
     6841 aagtcgttga ttacgtattg ggtttccacc cgtcggctca atcgccgtca accctgttat
     6901 cgtctgtcgt aaaacaacct ttagaatttt tttcacaaac agccattttt tgttagtcgc
     6961 cgaaatactc ttttctctgc cccttattcc cgctattaaa aaaaacaatt aaacgtaaac
     7021 tttgcgcaat tcaggccgat aaccccggta ttcgttttac gtgtcgaaag ataaaaggaa
     7081 atcgcatggc aagtatttca tcgctgggag tcgggtcagg tctggattta agttcaatcc
     7141 ttgatagcct caccgccgcg caaaaagcga cgctaacccc catttcaaat cagcaatcgt
     7201 cgtttaccgc taaacttagc gcctacggta cgctgaaaag cgcgctgacg actttccaga
     7261 ccgccaatac tgcattgtct aaagccgatc ttttttccgc caccagcacc accagcagca
     7321 ccaccgcgtt cagtgccacc actgcgggaa acgctatcgc cgggaaatac accatcagcg
     7381 tcacccatct ggcgcaggcg caaaccctga ccacgcgcac caccagagac gatacgaaaa
     7441 cggcgatcgc caccagcgac agtaaactca ccattcaaca aggcggcgac aaagatccga
     7501 ttaccattga tatcagcgcg gctaactcgt cgttaagcgg gatccgtgat gccatcaaca
     7561 acgcaaaagc gggcgtaagt gcgagcatca ttaacgtggg taacggtgaa tatcgtctgt
     7621 cagtcacatc aaatgacacc ggccttgata atgcgatgac actctcggtc agcggtgatg
     7681 atgcgctaca aagttttatg ggctatgacg ccagtgccag cagcaacggt atggaggtct
     7741 cggttgccgc ccagaatgcg cagctgacgg tcaacaacgt cgccatcgag aacagcagca
     7801 acaccatcag cgacgcgctg gaaaacatca ccctgaacct gaacgatgtc accacgggca
     7861 accagacgct aaccatcact caggacacct ccaaagcgca aacggcgatt aaagattggg
     7921 tgaatgccta taactcgcta atagatacct tcagcagcct gaccaaatac accgccgtag
     7981 atgcgggagc tgatagccag agttctagca atggcgcact gctcggcgac tccacgttgc
     8041 ggacgattca gacgcagttg aaatcgatgc tgagtaatac catcagttct tccaactata
     8101 aaacgctggc gcagattggt atcacgaccg atcccagcga tggcaaactg gaactggatg
     8161 ccgacaaact caccgctgca ctgaaaaaag atgccagcgg cgtaggtgca ttgattgttg
     8221 gcgatggtaa aaaaaccggc atcacgacca ccatcggcag caacctgacc agttggcttt
     8281 cgacaacggg cattattaaa gccgctaccg atggcgttag taaaaccctg aataagttaa
     8341 ctaaagacta caacgccgcc agcgatcgca ttgatgcgca ggtcgcgcgc tataaagaac
     8401 aatttaccca actggacgtt ttaatgacct cgttaaacag cacaagcagc tacttaacgc
     8461 agcagttcga aaacaacagt aattccaaat aagcaatatt catcgggaga caggtcatgt
     8521 acgcggcaaa aggcacccag gcctatgcac aaattggcgt cgaaagcgcc gtaatgagcg
     8581 ccagccagca gcagctggtc accatgctat ttgatggagt gctgagcgca ctggttagag
     8641 cgcgcctgtt tatgcaggac aacaatcagc aaggcaaagg cgtctctttg tcaaaagcga
     8701 tcaacatcat tgagaacgga ctgcgggtga gtcttgatga agagagcaaa gacgaattaa
     8761 cccaaaactt gattgctctt tatagctata tggtcaggcg tttgctgcaa gccaatttac
     8821 gcaacgatgt ctccgcagtc gaagaagtgg aagcattaat gcgcaatatt gccgatgcct
     8881 ggaaagagtc gttactctcc ccttctttga ttcaggaccc agtctgatga accatgcacc
     8941 gcatttgtat ttcgcctggc aacaactcgt cgaaaaaagc cagctcatgt tacgcctggc
     9001 aacggaagaa caatgggacg aactcatcgc cagcgaaatg gcgtatgtga atgcggtgca
     9061 ggagattgca catctgactg aagagattaa cccatccacc acgatgcagg agcagctccg
     9121 cccgatgctg cgcctgattc tcgacaacga aagcaaggta aagcagttat tacagattcg
     9181 gatggatgaa ctggcgaaac tggtcggtca gtcatcggtg caaaaatcgg tgttaagtgc
     9241 ctatggcgat cagggcggct ttgtgctggc tccgcaagat aacttctcgt gaatcttaac
     9301 aagctaatgg ctcgcgaata atctgattac ggctacgctt ctaatgttcc ccttgaatgg
     9361 agtcgaagaa tgcgtaatcc cacgctgtta caatgttttc actggtatta cccggaaggc
     9421 ggtaagctct ggcctgaact ggccgagcgc gccgacggtt ttaatgatat tggtatcaat
     9481 atggtctggt tgccgcccgc ctataaaggc gcatcgggcg ggtattcggt cggctacgac
     9541 tcctatgatt tatttgattt aggcgagttt gatcagaaag gcagcatccc cactaaatat
     9601 ggcgataaag cacaactgct ggccgccatt gatgctctga aacgtaatga cattgcggtg
     9661 ctgttggacg tggtagtcaa ccacaaaatg ggcgcggatg aaaaagaagc tattcgcgtg
     9721 cagcgtgtaa atgctgatga ccgtacgcaa attgacgaag aaatcattga gtgtgaaggc
     9781 tggacgcgtt acaccttccc cgcgcgtgcc gggcaatact cgaagtttat ctgggatttc
     9841 aaatgtttta gcggtatcga ccatatcgaa aaccctgatg aagatggcat atttaaaatt
     9901 gttaacgact acaccggcga aggctggaac gatcaggttg atgatgaatt aggtaatttc
     9961 gattatctga tgggcgagaa tatcgatttt cgcaatcatg ccgtgacgga agagattaaa
    10021 tactgggcgc gctgggtgat ggaacaaacg caatgcgacg gttttcgtct tgatgcggtc
    10081 aaacatattc cggcgtggtt ttataaagag tggatcgaac acgtacagga agttgcgcca
    10141 aagccgctgt ttattgtggc ggagtactgg tcgcatgaag tcgataaact gcaaacgtat
    10201 attgatcagg tggaaggcaa aaccatgctg ttcgatgcgc cgctgcagat gaaattccat
    10261 gaagcatcgc gcatggggcg tgactacgac atgacgcaga ttttcacggg tacattagtg
    10321 gaagccgatc ctttccacgc cgtgacgctc gttgccaatc acgacaccca accgttgcaa
    10381 gccctcgaag cgccggtcga accgtggttt aaaccgctgg catatgcctt aattttgttg
    10441 cgggaaaatg gcgttccttc ggtattctat ccggacctct acggtgcgca ttacgaagat
    10501 gtcggcggtg acgggcaaac ctatccgata gatatgccaa taatcgaaca gcttgatgag
    10561 ttaattctcg cccgtcagcg tttcgcccac ggtgtacaga cattattttt cgaccatccg
    10621 aactgcattg cctttagccg cagtggcact gacgaatatc ccggctgcgt ggtggtcatg
    10681 tcgaacgggg atgatggcga aaaaaccatt aatctgggag agaattacgg caataaaacc
    10741 tggcgtgatt ttctggggaa ccggcaagag agcgtagtaa ccgacgaaaa cggtgaagcg
    10801 accttctttt gcaacggcgg cagcgtcagc gtgtgggtta tcgaagaggt gatttaaatt
    10861 catccccggc ggcaagccgg ggagatttca ttacggcagt ggcgtcggta acggcgcttt
    10921 atccagcgcg gcggcacact ctgccgtcgg gcgatccaca cgctccatcg tcataccatc
    10981 gtactcaatg gtgttaccgt cacgctcaac ctcgtacagc tcacgtttta cggtaacgtt
    11041 agtgagatca tctgacatca atgtcaactt gcccggcacg gcaatcaccc gctgccactg
    11101 acggcaatcc agggtatcgc cctctttagt gacaatcagg ctgcctatcg cttccggact
    11161 gaccagcgca cgttgcggcc ctttggtctg ccagtagccc gccagccagt ccggcgcggg
    11221 ggttttcacg acattgttat agttttcgac ttcggcacag cccgccagca acatgagcgc
    11281 gcctgcaatt gcgagttttt tcatcattcg tcctgcatga gaagaaaaga ggattgtggc
    11341 attaaagccc tgatgtcgcc agcctttcca ggttcagaga gaaatttgat ctaactatta
    11401 tttttgtgaa ttattacccc gaaaataaat ttgcatgatg tatgattcgc gactcttgtt
    11461 tcaaaccttc tgagttcaga ggctacattc atgtcatggc agcaattcaa acacgcctgg
    11521 ttgattaaat tctgggcacc catccctgcg gtcatcgcgg cgggtattct ctccacttac
    11581 tattttggca ttactggcac cttttgggct gtcacgggtg aatttacccg ttggggcggc
    11641 cagctcctgc agctgtttgg cgtccatgct gaagagtggg gttattttaa aattatccat
    11701 ctggaaggat cgccattaac ccgcatcgac gggatgatga tcctcggcat gtttggcggc
    11761 tgctttgccg cagcgctgtg ggccaacaat gtcaaactgc gcatgccacg cagccgtatc
    11821 cgcattatgc aggccattat tggcggtatt atcgctggtt ttggcgcgcg tctggcaatg
    11881 ggctgtaacc tggcggcgtt ctttaccggt attcctcagt tctcgctgca tgcctggttc
    11941 tttgccatcg ccactgccat tggttcatgg tttggcgcgc gctttaccct gctgcccatc
    12001 ttccgtattc ccgtgaaaat gcagaaagtt tctgccgcct caccgctgac gcaaaaaccg
    12061 gatcaggcgc ggcgtcgttt tcgtctcggg atgctggtct ttttcggcat gctgggctgg
    12121 gcgctgctca cggcgatgaa ccaacccaaa ctggggctgg caatgctgtt tggcgtcggc
    12181 tttggtttac tgattgaacg tgcgcaaatc tgctttactt cagcgttccg cgatatgtgg
    12241 atcaccggac gtacccatat ggcgaaagca atcattattg gtatggcggt gagtgccatc
    12301 gggatcttca gttacgtaca gttaggcgtt gaacccaaaa tcatgtgggc gggaccaaac
    12361 gcggtaattg gtggtttact gtttggtttt ggcatcgtgc tggcaggcgg ctgcgaaacc
    12421 ggctggatgt accgcgcggt agaaggccag gtgcactact ggtgggtcgg tctgggcaac
    12481 gtgatcggct caacgattct ggcgtattac tgggatgatt tcgctccggc gctggccacc
    12541 gactgggaca aaatcaacct gctgaaaacc tttggcccga tgggtggcct gctggtgaca
    12601 tatttgctgt tgtttactgc gctgatgttg attatcggct gggaaaaacg cttcttccgc
    12661 cgtgcggcac cgcagactgc taaggagatc gcatgaaaaa tatcgttcct gattaccgcc
    12721 ttgatatggt gggtgaaccc tgcccttatc cggcagtcgc aactcttgag gcgatgccgc
    12781 agttaaaaaa aggggaaatc ctggaagtgg tgagcgactg tccacagtcg atcaataata
    12841 ttccactgga tgcgcgtaat cacggctata cggtgctgga tattcagcaa gacgggccga
    12901 ccattcgtta tttaattcag aagtaatccc tcattcccgc tgtggcccac agcgggaaca
    12961 tcgttacctt tgtatcacat agattttacc cgccccctct cctattcttt tcagtatgat
    13021 ctcaggaggt ttctatgtgt ggacgctttg cccaatccca aacgcgtgta gattaccttg
    13081 cgcttctcgc ggaagatatt gaacgcgata ttccctatga tcccgaaccc attggcagat
    13141 acaacgtcgc gccgggaacc aaagtccttc tgctcagtga acgtgacgaa caccttcatc
    13201 tggatccggt tttctgggga tatgctcccg gatggtggga taaaccgcca ctgattaacg
    13261 cccgcgtaga aactgccgca accagtcgca tgtttaaacc gctctggcaa catggtcggg
    13321 caatctgttt tgccgatggc tggtttgagt ggaaaaaaga aggcgacaaa aaacagcctt
    13381 attttatcta tcgcgctgat ggacaacctg tttttatggc cgcgataggt agcacaccgt
    13441 ttgagcgtgg tgacgaagcc gaaggatttt taattgtcac tgctgcagca gaccagggtc
    13501 tggtagacat tcatgaccgc cgcccactgg tactgtcgcc agaagctgcg cgggaatgga
    13561 tgcggcaaga gattggcggt aaagaagcat cagaaattgc taccaacggc tgtgtgccag
    13621 caaaccagtt cacctggcac ccggtatcgc gcgcggtagg taatgtcaaa aaccagggcg
    13681 cggagttaat tcaacctgtt tgttgacatc ctccacgccc tgaaggacga ggatccctgc
    13741 tacgttcagg ctgtcgcctg aatcatttcg gtgggttcct gcttcaacgg gcggcctgac
    13801 tgcaccatct ctccacaggc aagaacggcg tgcccc
//
LOCUS       DAZGAN020000125        13720 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGAN020000125 DAZGAN020000000
VERSION     DAZGAN020000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.125"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHKYO_RS20450"
                     /old_locus_tag="pgaptmp_004090"
     CDS             complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHKYO_RS20450"
                     /old_locus_tag="pgaptmp_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGU4430851.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGA"
     gene            516..1217
                     /locus_tag="ACHKYO_RS20455"
                     /old_locus_tag="pgaptmp_004091"
     CDS             516..1217
                     /locus_tag="ACHKYO_RS20455"
                     /old_locus_tag="pgaptmp_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HGU4430852.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHKYO_RS20460"
                     /old_locus_tag="pgaptmp_004092"
     CDS             1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHKYO_RS20460"
                     /old_locus_tag="pgaptmp_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HGU4430853.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1616..2926)
                     /gene="cyuA"
                     /locus_tag="ACHKYO_RS20465"
                     /old_locus_tag="pgaptmp_004093"
     CDS             complement(1616..2926)
                     /gene="cyuA"
                     /locus_tag="ACHKYO_RS20465"
                     /old_locus_tag="pgaptmp_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HGU4430854.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDSTAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWHGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2954..4285)
                     /locus_tag="ACHKYO_RS20470"
                     /old_locus_tag="pgaptmp_004094"
     CDS             complement(2954..4285)
                     /locus_tag="ACHKYO_RS20470"
                     /old_locus_tag="pgaptmp_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4430855.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLRSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4560..5924)
                     /gene="tdcG"
                     /locus_tag="ACHKYO_RS20475"
                     /old_locus_tag="pgaptmp_004095"
     CDS             complement(4560..5924)
                     /gene="tdcG"
                     /locus_tag="ACHKYO_RS20475"
                     /old_locus_tag="pgaptmp_004095"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU4430856.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HLVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFTRIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(5996..6385)
                     /locus_tag="ACHKYO_RS20480"
                     /old_locus_tag="pgaptmp_004096"
     CDS             complement(5996..6385)
                     /locus_tag="ACHKYO_RS20480"
                     /old_locus_tag="pgaptmp_004096"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HGU4430857.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6399..8693)
                     /gene="tdcE"
                     /locus_tag="ACHKYO_RS20485"
                     /old_locus_tag="pgaptmp_004097"
     CDS             complement(6399..8693)
                     /gene="tdcE"
                     /locus_tag="ACHKYO_RS20485"
                     /old_locus_tag="pgaptmp_004097"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HGU4430858.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8727..9935)
                     /gene="tdcD"
                     /locus_tag="ACHKYO_RS20490"
                     /old_locus_tag="pgaptmp_004098"
     CDS             complement(8727..9935)
                     /gene="tdcD"
                     /locus_tag="ACHKYO_RS20490"
                     /old_locus_tag="pgaptmp_004098"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HGU4430859.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(9961..11292)
                     /gene="tdcC"
                     /locus_tag="ACHKYO_RS20495"
                     /old_locus_tag="pgaptmp_004099"
     CDS             complement(9961..11292)
                     /gene="tdcC"
                     /locus_tag="ACHKYO_RS20495"
                     /old_locus_tag="pgaptmp_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HGU4430860.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11314..12303)
                     /gene="tdcB"
                     /locus_tag="ACHKYO_RS20500"
                     /old_locus_tag="pgaptmp_004100"
     CDS             complement(11314..12303)
                     /gene="tdcB"
                     /locus_tag="ACHKYO_RS20500"
                     /old_locus_tag="pgaptmp_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HGU4430861.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12402..13340)
                     /gene="tdcA"
                     /locus_tag="ACHKYO_RS20505"
                     /old_locus_tag="pgaptmp_004101"
     CDS             complement(12402..13340)
                     /gene="tdcA"
                     /locus_tag="ACHKYO_RS20505"
                     /old_locus_tag="pgaptmp_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HGU4430862.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            13517..>13720
                     /locus_tag="ACHKYO_RS20510"
                     /old_locus_tag="pgaptmp_004102"
     CDS             13517..>13720
                     /locus_tag="ACHKYO_RS20510"
                     /old_locus_tag="pgaptmp_004102"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430863.1"
                     /translation="MSKKMTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFEL
                     NLMSKFSNFIINKPFSAINTAARH"
BASE COUNT         3619 a         3664 c         3195 g         3242 t
ORIGIN      
        1 cgcccccgcc agcacttcgg tgatgattgc cagttgggta ttggcttttg ccgccagttt
       61 gtcgattaac gggaaaaagg caggtgtcgg taccagcgcc tcggtcacaa tggtgagatg
      121 cgtttcccaa ccgcgcgcga gggcttccgc atcggtagtc agtttatctg cggcttccag
      181 caaaacgcgc cctcgctcca gcaacatcct cccgacattg gtgaatttgg tgcgatggcc
      241 cgagcggtca aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag
      301 tgcggaaggc acgcgtccca gctcatccgc cgccgccgca aaactgcccc ggcgatcgat
      361 cgcatccata acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc
      421 attcaggaaa tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc
      481 ttaccataaa aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccagac
      541 agtgtggaca agcagactac ggatggttgc aggcccggta tactttttcc tttggacact
      601 acttcgaccc gaaattgtta ggctatgcct ccctgcgcgt gcttaaccag gaagtgctgg
      661 ccccaggtgc cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc
      721 tggatggaga agcagagtat cgcgacagcg aaggcaatca tgttcaggcc agcgccggtg
      781 aggcgttgct gctctctacc cagccgggtg tcagctacag cgaacacaat ctcagcaaag
      841 acaaaccgtt aacacgaatg cagctttggc tggacgcctg cccgcagcga gagaatccgc
      901 tgattcaaaa actggcgctt aatatgggca agcagcaatt aatcgcctcg ccagagggta
      961 cgatgggaag cctgcaatta cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag
     1021 gcgaaagtgc gaatttccag ttgcatgggc cacgcgcgta tttgcaatcg attcacggga
     1081 aatttcatgc gcttacgcat catgaagaga aagcggcgct gacctgcggt gatggggcgt
     1141 ttattcgtga cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga
     1201 tagatttgcc tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa
     1261 aagcgccagc cagtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt
     1321 ggctatgaag agatgttgag cgagctggaa gccatcgtcg cggatgctga aacgcgttta
     1381 gccgaggatg aagctaccgc gtaaagacag gttgccggat gcggcgaaaa cgccttatcc
     1441 ggcctacaaa atccaacaac attaacgaat taaacaaccc gtaggccgga taagatgcgc
     1501 cagcatcgca tccggcatca ggtacaaaac acaacaacat caacaagttg agcaccccgt
     1561 aggccggata aggtgcgcca gcatcgcatc cggcacaata ctcaaaacct ggcgtttatc
     1621 tggccttgct cgccataatc tcgataatct gccgatccgt ttgctgcatc gaatggcttg
     1681 ctaacgcaca caggttggca atcgactgct caacatcatg cgccacgatc ccttcattgc
     1741 cggtcacggc ggtatcatcc agcgccatta acaccgcttt ccacgcagcc gaagcactgg
     1801 tcgaaacctt catcgcgcag ctgttcgacg caccatcgca aatcatgccg ctgacatcgc
     1861 cgatcatact gctgatcgcc atcgagatgg tttcataacg cccatccacc agccatgcca
     1921 tcccggcggc ggcccccatt gctgcggtcg ttgcggcaca cagcgcagac aaacgcggta
     1981 actggttatg gatgtaaatt gcgctcaaat gagaaagcat cagcgcacgc gccagccgtt
     2041 catcatccgc tccgaagtgt tctgctacca ccaccacagg cattgttgcg gtgatcccct
     2101 ggttacccga gccggagtta ctcatagccg gaagcgtagc gccgcccata cgcgcatcgg
     2161 atgccgcgct ggtacgaatc acaatggatg aagagagatc tttcgccagc aaaccgcgct
     2221 cgcactgttt ttccagcgtc gcgccaatat gcagccccca cttaccgctc aaaccttcct
     2281 gcgataacgc acaatttagc ttcgcggaat cgagaataaa gcggatcgcc gcaaacggga
     2341 cttcattgac gaacttcagg atctcagcca gcgtcgttct ggaaagcacc gtcagcggag
     2401 actcttgctc gccctctgcc acacacgcct gctgggtaaa caccacacca ttgtgcgtct
     2461 caatatgcac aatgttggta tgcccgccga cgatggtgac acacgcccac ttctcaccgt
     2521 gccagacttt ggcgcgtgag aagaggattt cattgcaagg ttcctggatc ttaacggaga
     2581 ctttccccgc cgccagcagt gctttggcat cggcaattgc ctgcgcagtt gagtctttca
     2641 gcacttccag cccggcgttg gcatttccac ctaacgcccc cagcgccgcc gcaatcggca
     2701 gccccaccat tcccgtgccg ggaacggtga cgcccagacc gttcttcatc agatttggcg
     2761 aaacccaggc ttctacacgt tcaaccggac cttccagttc tgccgcagca accgccgccg
     2821 ccagcgccag tgaaatcggt tcagtacatc ccagcgccgg ttttacttcc tcctgaactg
     2881 caaggatgta acgctgccat aacggattta aagtcgaatc aaacatatta aaaaccttaa
     2941 aatttcaggt aaatcaagaa aatgccagga acggagaaac acaaagcaac aaaccagtga
     3001 cgataatcag gtacagagac attcctttgt atttgtgcaa tgccggtact ttgtaaacca
     3061 gccacgccgg gatcaggcac cctaccatgc cgaaaatcgg gctacagata gaggtgaagc
     3121 tcaacaccgg tgcgttcagt acgatggcgc tccaggccag caaaatggcg aaaatcatga
     3181 tgccgcgctg aacaagattt tcgttaatct tctcggcagg catcttgcga cgcaggatgt
     3241 tcattacgat cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag aatgcagtca
     3301 tgacagcaaa gatattgaga atgacgctga ccactttcac ccacgctgcg ccgtcaccgc
     3361 taataaactg tgcggcaatc gccagcgcgg aaatattctg ctcataggct tttaccgctt
     3421 cgtcatgtcc catcgccagc gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa
     3481 tgccaaacgc gatattcatt gcccgcaatg ctttatgccg cgccacttca attgattttt
     3541 cccgcgagcg ataagagatc accatcgggc ttaacgtctg gataaacaga atcgacgtca
     3601 gggtaaacgg cagcgtaata atggcgtttt tcaccagcag ccccagcggc ggtagcgaac
     3661 cgacgttata cagatgccac attcccacca tcgacacgcc cagcgccgcg accaccagca
     3721 gcttggtcag caccatgccg gtcgaaattt tgaataacaa tttctcgccg cgtgaagaga
     3781 tcgccaccag aatgcaaatc agtaccagac cataaaaggg actgtctgac cgcaaccctt
     3841 ccgtcacgcc gaaggtatgc agataggaag cactatcgtt ggtgatggcg gtggaataga
     3901 cgaacatcca aatcaccagc attacgaaat agagcgcacc taacaggatg ccccagtttt
     3961 tacctaaata accgctaatg acactcgggt aatctttaca ttctggtgat tctgccagcg
     4021 tattaataaa caaccgctga aacagataca ttgccgggta accaatcacc gatgagagca
     4081 aaaataccca caatcccatc aaaccgacct gcaccgggag aaaaacaatc cccgcgccaa
     4141 tcgccatccc gatactcata atcacccagc cggtgtcagt actgtcgaat ttgatcgctt
     4201 ctcgccactc gctctcactc attccagcac gacccgccgg ggtcgaagcg tctgcaatga
     4261 cgcctttatt cgatgcaatt tccataattt ctcgctcaat attttgtagg gcttattatt
     4321 tttttccgag ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga gttattagaa
     4381 taaagaaatg atacgcgcga ggtcagagaa aatcttcaca atcaagactt tgaaataacg
     4441 gagatggata agaattttct actaaattaa tcgcaagaga atattcattc tctatttgcg
     4501 gcagatcaca aaaaaaaggt gcacatttgt gcacccaagg atgaaagctg acagcaatgt
     4561 cagccgcaga ccactttaat ggccagtcct ccgcgtgatg tttcgcggta tttatcgttc
     4621 atatctttgc cggtttcata catcgtctcg atcactttat cgagtgaaac acgcggtgcc
     4681 gaggtgcggc gcatcgccat ccgcgcggcg tttactgctt tcacggcatt aatggcatta
     4741 cgttcaatgc acgggatttg tacctgtccg gcaaccggat cgcaggtcag cccaaggtta
     4801 tgctccatcg cgatttccgc cgcattgcat acctgcgccg gactgccgcc cagtagttca
     4861 gttaaccctg ccgccgccat tgaacaggcc acgccaatct ccccctgaca gccgacctcc
     4921 gcgccagaga tggaggcgtt cattttatac agcgcgccaa tagcccccgc tgccagaaaa
     4981 tagcgggcaa ttgaccgctc gtttaccgga cgacggaact tatcgtaata agccagcact
     5041 gccggaataa tgccgcacgc accgttagtc ggtgccgtta ccacgcgccc gccagctgcg
     5101 ttttcttcac taaccgccag cgcgtacatg ttgatccagt cgatgacatt catcggatcg
     5161 ttagagatgt tatcgctaga aaccagctga cgacgcagcg ctacggcacg acgcggcaca
     5221 ttgagcggac caggcagcac gccttcagtg ttcatcccgc gttcaatacc gtcatgcatc
     5281 acttgccaga tacgggtaaa ccctgcgtca atttccgctt tactgcgcag cgctagctcg
     5341 ttgtgcatca tcaggccaga tatagacagg ccgttgtaat cacacatttt cagcagttca
     5401 cctgctgagt ggaaatcgta aggtacggac gtttcgacat cgtgcgacag gccgaagtgt
     5461 tcttcttcga caataaaccc gccgccgaca gagtaatagg ttttacttaa tagcgcttcc
     5521 tgtcccttcc aggcagtgat ccgcattccg ttctcatggc gaggcaacat ttcgggatgg
     5581 aagataatgt tctttgctac aggaaaatca acaagatgcg caccagatgc cactggcagc
     5641 cgcccgctgc gcgttactaa ctctataaat gcagggatct catcaatgac aacatcctgc
     5701 ggactgtttc ctgccagtcc catgataatg gcgacatccg tggcatggcc tttgcccgtc
     5761 agtgacaacg acccatacag atcgaccaca atatggctcg tcgcggttaa taagccgcta
     5821 ctttccagcc gatcaataaa actttttccg gcattcattg gccccacggt atgcgaactg
     5881 gagggaccaa tcccaatttt gaaaatatcg aatgcactaa tcatatccac accctcggat
     5941 tgccgttcag tgaagtggag cggaacgacc ttgcgaccgt cccgctcacg aggctttacg
     6001 cactacgtac tgcgatagct tcaatttcca gcttcacatc tttcggcaaa cgcgcaacct
     6061 gcacacagct ccgggtcggg taggtcgcct gatgctcatc gaagaactgc ttatagactt
     6121 cattgatggt ggcaaaatca ttcagatcgg tgatgaacac ggtcatcttg atgatatcgc
     6181 ccacgctcag cccggcagca accacgatcg ctttgacgtt ttcgaggctt aaacgcgcct
     6241 gatcttgcac atcagccggg atctcaccgg tctgcgggca aaccggaatt tgcccggagg
     6301 tgaagaccat gctgcctaaa tcaacgccct gaacataagg gccgattgcg cctggggcac
     6361 gttgcgtttc gataatcttt ttcatacatc ctccggcgtc agagcgcctg ggtaaaagta
     6421 cgtgaaataa catcctgttg ctgttcacgg gtcagtgcgt tgaagcgcac ggcgtagcca
     6481 gagacacgga ttgtcaggtt aggatatttt tccgggtgct cgatggcatc cagcagcatt
     6541 tcccgattca ttacgttgac gttgaggtgt tgaccgcctt cgacatccgc ttcgtggtgg
     6601 aaatacccat ccagcaggcc gacaaggttg gttttacgta ctggatcttc tttgcccagc
     6661 gccgcaggaa cgattgagaa ggtgtacgag atcccatctt tggcgtaggt gaacggcagt
     6721 ttcgccaccg acgtcaatga ggccacggca cctttgcggt cacgaccatg catcgggtta
     6781 gcgcccggcg cgaacggtgt tccggcgcga cgaccgtccg gcgtattacc ggttttctgg
     6841 ccgtacacca cgttagaagt gatagtcaga atcgactggg tagggacggc gttgcgatag
     6901 gttggcagcg ctttaatttt cttcataaag cgttcaacca ggtcgcaggc aatgctgtct
     6961 acgcgctcgt cgttgttgcc gtactgcgga tattcaccgt cgatttcaaa gtccaccgct
     7021 aggccgtttt cgtcacggat tggtttcacg cgggcatatt tgatggcaga cagggagtcc
     7081 gtcgccaccg acaggcccgc gatgccgcat gccatagtgc gatagacatc acgatcgtgc
     7141 agcgccatca gcgaagcttc gtagctgtac ttgtcgtgca tgtagtgaat gatattcagc
     7201 gcgctgatgt actgcaccgc cagccagtcc atgaagtgat cgaggctgtc catcactttg
     7261 tcgtaatcca gcacgtcgtc catcagcggt gctgttttcg gcccgacctg aatcttcagc
     7321 ttctcgtcca ccccgccgtt aattgcgtag agcagcgttt tcgccaggtt agcgcgtgca
     7381 ccaaagaact gcatttgctt accaatcacc attgggctga cgcagcaggc aatcgcgtaa
     7441 tcgtcgctgt tgaagtcagt acgcatcaga tcgtcatttt catactgcaa ggaagaggtg
     7501 acgatcgaca cctgcgcggc atattttttg aaggcaatcg gtaattcttc cgaccaaaga
     7561 atggtcaggt taggttccgg tgccggcccc atagtgtgca gggtgtgcaa atagcggaag
     7621 gagtttttgg tcaccagcgt acgaccgtcc agccccatcc cgccgatcac ttccgtcgcc
     7681 cagattgggt cgccggagaa cagcgaatca aattccggtg tacgcaggaa gcgtaccata
     7741 cggatcttca tgatgaagtg atcgatcagt tcctgtgcct gctgctcatt gagtacgcca
     7801 gctttaaagt cgcgctcaat gtagatatcg aggaacgatg ccgtgcggcc cagcgacatc
     7861 gcgccgccat tttgcgattt cactgccgcc agataagcga agtagagcca ctgcaccgct
     7921 tcctgcgcat tctgcgccgg gcgagagata tcaaagccat atttcgccgc catttcctga
     7981 atctgcaaca gcgcatgacg atgctctgcc agctcctcac gcagacggat ggtggcttcc
     8041 agatcctcgc ctttttccag acgagactgg agatcggcaa attgcagttc gcgttcacgt
     8101 accagataac tgatgccata cagcgctacg cggcgatagt caccgataat gcgcccacgg
     8161 ccatagccat ctggtaaacc ggtcagcacg ccagatttac ggcagcgcag catatccggt
     8221 gagtaaacat caaatacgcc ctggttatgg gttttacgca gatcggtaaa cagatattca
     8281 aattcactgt ccatttcacg gccataggcg tggaatgaac ttttaatcat attaatgcca
     8341 ccgaacgggt gtagcgcacg tttcaacggc gcatccgttt gcaggccaac aattttttcc
     8401 agcggctggt taatatatcc cgcatcatga gcggtaattg tggtggcaat attggtatcg
     8461 aaatcaaccg gcgcgtgggt tgcgttttcg atacggatac cttccattac tttttcccac
     8521 aattccgtgg tggcaggcgt cgcttcggcg aggaaagatt catcgccttc atacggtgta
     8581 tagttatgtt gaataaaatc gcggacatta atttcgtttt tccagtccgt acctttaaag
     8641 ccaagccatg cgtcggcgta cagcttatcg ctggtatcaa tatctacctt catgaaaaat
     8701 aatctctcta caatacttca actaaattat gcaaattctg cgggcgcgtt aactttgcct
     8761 aaatgaatgg catccaaagc aatcattttt tcttcgttag tcggaataac agcgcaaatg
     8821 acacgcgcat tttcactgga aacaattcgc tcaccacagg agttagagcg attattcatt
     8881 tctgtatcaa tctctacgcc taatacagcc aaatgttcca tgaccagacg acgaattaag
     8941 cttgaattct ctcctattcc gccggtgaat ataattccat ccaggcgacg taatgaagct
     9001 gcgtgtccgg caatatgacg ggcaattcgg tgaacaaagg ttttaattgc cagttgcgcg
     9061 cgttcgtgac cttcatgcca ggctttttcc agaacacgta aatccgaaga aagaccggaa
     9121 atacctaata atcccgactc tttattcact acgcgttcca ggtcacccag gctctggttg
     9181 gtttggctgg cgacccagga catcgcacca aagtcgacat cgccactgcg ggtacccatc
     9241 atcaagcctt ccagcggcgt cattcccatt gaagtatcaa cactctgacc gttgcgaacc
     9301 gcgcagattg acgcgccatt gccaagatgc gccacaacca ggccggagtc atcttccgcc
     9361 agattcagca gcgaatgtgc gcgctgggaa acatagcggt gcgacgtgcc gtggaaacca
     9421 taacggcgta cacctaactc ttcataatat ttccacggca ggccgtataa ataagcttcc
     9481 ggagccatcg tctggtggaa actggtatca aataccgcca cctgagttac gcccggaaat
     9541 aattgctgcg ccgattcaat accacttaaa ttggcgtaat tatgcagggg tgccagtgga
     9601 gaaacgcgac ggatattatc aatgacttca tcggtaataa tggcggactc ggtaaaaata
     9661 ctgccgccgt gagcgatgcg gtggccaatt aaggccacac tgtcatttaa attccgtttt
     9721 tccagttcaa atgcaattgc cttcaatgca ccttcgtagc tgtggtgagc cagcggtgct
     9781 ggctctcccc catttacgga taagaatgca ttttccgagt taataccgtc ggcaatacct
     9841 gacattaata cttcacagtc gctggcatcg agtacggaaa acttaatcga agacgaacca
     9901 cagttaataa ccaaaacaac cggaaattca ttcatctctt ttctcatcct gagttacgga
     9961 ttaaaacagt ttgtatacga tgttcaggat ggtcagcaga ccaatcacgg taacaaacac
    10021 gttatccaga cgaccacggt atttcgccag agacggcgct ttacggatgg catacatcgg
    10081 caacaggcac agcagggatg cgataatcgg tgcgcccatg gcttcaataa ggtcgaggat
    10141 gttcgggttg gcgtaggcaa caacccaggt ggagcccatg atgaagatca tgctgatagt
    10201 gttcagttta cccagcgaca ctttagtttt gtcgccttta taaccgaact tcagaatcag
    10261 accattcaag ccttccagcg tccccagata gtgaccgaag aaagatttga agatagccac
    10321 gagtgcgatg atggaagccg catattccag tgtaatcgcg aacgttgttt tggtaccggt
    10381 catggacgca aagtggttag ccagataaga aagcactgga atattctgcg ctttggcttc
    10441 cgccatgttg gccggagaca gcgtaaacag gcagctaaag gcaaagaaca tcaccactgc
    10501 aaccatcagc atgctggcac gagaaatgat ttgggaacat ttacgttcgg tgaagtcgcg
    10561 accgaagtct ttctcatact cttcacgttt agaaaccacg aaggaagaga cgattggcga
    10621 gaagttaaag gagaaaacca tgatggaaat ccccagccag acagtgatca ggataccgtc
    10681 atgaccggtt aacgacagcg aaccgaggtc aacctggtcg ataactgcag agttccagta
    10741 agggatcagc gacagagaaa tcagcaccag gctggcgata aacggccata ccaggtagct
    10801 catcacttta accatcagat ccttaccaaa ccagatgacg aaagccatca gcagcaacag
    10861 gaacagcgcc acaaagccgc gattcagcgg tgcaaatccg agctggtttt cccagaacgt
    10921 cataaaggta ttggtaatag taacgccata aatccacagc agtgggcaaa tcgcgaagaa
    10981 gtacaggaac gtgataacca cgccgccagt tttaccaaaa tgctcttcca ccgtttccgt
    11041 aatgttgccg gaagggttag agccagaaag acacagacgc gccagcgccc ggtggcaata
    11101 aaatgcgatg gggtatgcca ataccaacat cagaagaatc gggatcagtc cgccaaaacc
    11161 tgcgcggata gggaagaaca gcaccccggc gccgattgcc gtaccaaaca agcctaacgt
    11221 ccatgtggta tctgatttac gccaggacga ttgttttgtc tggctggata caatgctatc
    11281 tgaagtactc atatcctatc ctcaacgaat taattaagcg tcaacgaaac cggtgatttg
    11341 agagacgcga gaaagatcga tattgccgcc ggaaataata ctgacggttt ttctgttttg
    11401 aatatattgg tctaatttac cgcttaataa tgcagcacat gccagagcgc ctgcgccttc
    11461 ggtgacgact ttattgcgct gaattaaggc aatcatactg tttctgattt cgtcttcgct
    11521 gaccagcacg atgtcatcga ctaattcacg aacgatttcg taagttaaat tacccgggcg
    11581 ggagacatca caaccatccg ccagggtgcc ggtagttcgg tgcgtggtta tttctccgga
    11641 gtggaaagaa gccgccatgc cgtgaacgtt ttctgactgt acgccaataa cacgaatggt
    11701 cgggttaata gatttaattg ccaccgcaat accagcaatt aaaccaccac caccaattgg
    11761 cacaatcacg ttatcgacat catagagatc ttccataatt tccagaccaa tcgttccctg
    11821 gccagcaatc actttcggat catcgtaagg tgggataaaa atacgacctt ccatttcgac
    11881 aatttcgctc actttagcga tagtgtcgtt gaaattatca ccatgcagaa cgacttctgc
    11941 ggagtagtcg cacgttgccg ctactttgga ttttggcgca ccttttggca tcaccacttt
    12001 accgtcgata cccagcatcg cgcaggagag cgaaacccct tgcgcatggt tgcccgcaga
    12061 acaggccact acacctttac gtttttccgc atcagtcagt gaacttaatt tattaaatgc
    12121 gccacgaatt ttaaatgaac ccgtacgctg catattttca aacttcagga atatttcacc
    12181 tttgcaacgt tcactaaaat agtttgagcg aggcatgcct gttttataaa ttcgcccagc
    12241 cagtcgttgt ttcgcttcaa taatgtcatc aatagcaacc ggcagatcgt atgtaatatg
    12301 cattataaaa cctcttcgcc tgaattaaat atgtaggtaa ccgtaaccga cacctgcaaa
    12361 acagacaggt ggattattta tgtttaaata aaaacaaata actaaccaac ttctattaat
    12421 tgccttcgtc tacacccatt ataagatgaa tactctttgg ctaattccac caaaaccgat
    12481 gctgcttttt taatacgata atttttcgac cataccgcgg catattgtgc cacaggtaat
    12541 gtttcttcaa ccggaatagt aataaattga tttgaaccaa aaggtgacgt catatcacaa
    12601 ggaattacag ttaagaaatc agcattgaga acaagattat aaattgtcac gactgagtcg
    12661 gttttaacga tgttttcaat actgatgcca tttctttgta acgtagtaag aagctcgctg
    12721 tagtacccca tattggtttg tggcaacacc cactgttcgt tcttcaacga ctccagcgtg
    12781 gtggtgccgg tgcatgttcg ggacttactg gctaccaaca caaactcgga ctcgaacagc
    12841 ggctcaacat gtaaatcctg aagcttcatt tctgcactta acgtaccaat cgcaaaatcc
    12901 agtcgaccgt cgcggattgc cggtaagaac gaagacagtt gcgcttcata catagaaacc
    12961 tgcgctttcg ggaacacctc tttgaacttg ttgatcatcc ctgacataaa agtaaaacca
    13021 atcaatgaag gaaaaccaaa tgagacttcc accaccgcct cagaagacat accgcttatc
    13081 tcattaacca tatttttcat ttcacgggta atggattcgg aacgggagag taacaattga
    13141 ccggcaggtg ttaatgttac accggtattt ttccgcacca ctaattccac accaaaataa
    13201 tcttcaatat cgttaatgat tttactgacg gctggttgag ttaaccctaa ttcttttgca
    13261 gccgagccga tagagccact tctaatgact tcctgaaaga ctaccaggtg ctgcgttttc
    13321 ggaagaagaa tagtgctcat aacgacctac gttaattacc tcattgacgg catgaagtgt
    13381 atcaaaatga aatgaacagg atatgtgcga ccactcacaa attaactttc aatactttcc
    13441 aaggtatcgt tattacaatt aaacaccttt aaatatcaac aagttaaagc ataaaaatca
    13501 gcataaaccc tgatttttgt caaaaaaaat gacggggata accatatttt atggcgataa
    13561 catcattcgt tatgtggtta atacaaaaaa ggggctgaga ccatatttca agcaattacc
    13621 ggataattac caggcaaaat ttgagttaaa tttaatgagc aagttctcaa attttattat
    13681 aaataaacca ttttcagcga taaatactgc ggcacgtcac
//
LOCUS       DAZGAN020000126        13718 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGAN020000126 DAZGAN020000000
VERSION     DAZGAN020000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.126"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHKYO_RS20515"
                     /old_locus_tag="pgaptmp_004103"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHKYO_RS20515"
                     /old_locus_tag="pgaptmp_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HGU4430864.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACHKYO_RS20520"
                     /old_locus_tag="pgaptmp_004104"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACHKYO_RS20520"
                     /old_locus_tag="pgaptmp_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HGU4430865.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHKYO_RS20525"
                     /old_locus_tag="pgaptmp_004105"
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHKYO_RS20525"
                     /old_locus_tag="pgaptmp_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HGU4430866.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTRARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHKYO_RS20530"
                     /old_locus_tag="pgaptmp_004106"
     CDS             complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHKYO_RS20530"
                     /old_locus_tag="pgaptmp_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HGU4430867.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="ACHKYO_RS20535"
                     /old_locus_tag="pgaptmp_004107"
     CDS             7014..7292
                     /locus_tag="ACHKYO_RS20535"
                     /old_locus_tag="pgaptmp_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430868.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="ACHKYO_RS20540"
                     /old_locus_tag="pgaptmp_004108"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="ACHKYO_RS20540"
                     /old_locus_tag="pgaptmp_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HGU4430869.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="ACHKYO_RS20545"
                     /old_locus_tag="pgaptmp_004109"
     CDS             complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="ACHKYO_RS20545"
                     /old_locus_tag="pgaptmp_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HGU4430870.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9446..9778
                     /gene="hdeA"
                     /locus_tag="ACHKYO_RS20550"
                     /old_locus_tag="pgaptmp_004110"
     CDS             9446..9778
                     /gene="hdeA"
                     /locus_tag="ACHKYO_RS20550"
                     /old_locus_tag="pgaptmp_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HGU4430871.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9894..10220
                     /gene="hdeB"
                     /locus_tag="ACHKYO_RS20555"
                     /old_locus_tag="pgaptmp_004111"
     CDS             9894..10220
                     /gene="hdeB"
                     /locus_tag="ACHKYO_RS20555"
                     /old_locus_tag="pgaptmp_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HGU4430872.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10284..10931
                     /gene="yhiD"
                     /locus_tag="ACHKYO_RS20560"
                     /old_locus_tag="pgaptmp_004112"
     CDS             10284..10931
                     /gene="yhiD"
                     /locus_tag="ACHKYO_RS20560"
                     /old_locus_tag="pgaptmp_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGU4430873.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTTAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLYATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10973..11503)
                     /gene="dctR"
                     /locus_tag="ACHKYO_RS20565"
                     /old_locus_tag="pgaptmp_004113"
     CDS             complement(10973..11503)
                     /gene="dctR"
                     /locus_tag="ACHKYO_RS20565"
                     /old_locus_tag="pgaptmp_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HGU4430874.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVNFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11659..12225)
                     /gene="slp"
                     /locus_tag="ACHKYO_RS20570"
                     /old_locus_tag="pgaptmp_004114"
     CDS             complement(11659..12225)
                     /gene="slp"
                     /locus_tag="ACHKYO_RS20570"
                     /old_locus_tag="pgaptmp_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HGU4430875.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(12579..13694)
                     /locus_tag="ACHKYO_RS20575"
                     /old_locus_tag="pgaptmp_004115"
                     /pseudo
     CDS             complement(12579..13694)
                     /locus_tag="ACHKYO_RS20575"
                     /old_locus_tag="pgaptmp_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3785 a         2952 c         3048 g         3933 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgcagtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggtggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttttgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcccg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagc gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
     6301 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcaac tgggtgcgcg cggtatcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcaattt gtaaatatca
     8041 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aatgatcact agcaggtaat tatttcaatg
     8521 atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg gatcatagtc
     8581 acatcaagtg actatgatcc gggtgacaac cgggataatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
     9121 attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt tgccttatct
     9181 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     9241 ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg cagtatctga
     9301 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca
     9361 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     9421 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     9481 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     9541 accggtcaac tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     9601 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     9661 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     9721 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     9781 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     9841 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     9901 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     9961 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
    10021 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
    10081 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
    10141 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
    10201 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
    10261 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
    10321 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
    10381 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
    10441 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
    10501 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
    10561 taggtctgac gacggcagcg gatatctggg tgaccaccgc cataggtatg gttattggca
    10621 gcggtatgta cgaactgggt atttatggtt cagtgatgac cttgttggtg ctggaagtct
    10681 tccatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
    10741 acggtaatac ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa acggatttag
    10801 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag ctttatgcca
    10861 caacgtcgat agaagacctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt
    10921 caattagcta atctgtgtgg cagcgtagcc agactcaccg taagcctgaa attcacacca
    10981 gataatcaat atgctgatgg cgtaccagct cattgatccg cttaagattg aggatcatca
    11041 tgatattgtg tttatggcag tagaacgttt ttagcgatat tttcagtttc tccagaatat
    11101 cttccgttgt catttgattc gcaatgcagc tcagaacctg atgttgcttg cgcgaaagtg
    11161 acacagccgc ttcaggcaca gagcagtaac tgccattgat gagtgcgaca aaatcaatga
    11221 tagtgatagt ccgcggaaca aagttcaccg gcactttccc caggcagcgt ttaatattaa
    11281 agggggagaa aataatcaca tggacaggtt ttaatcgttc aatttgagtg agaaaattag
    11341 aatgaaatac attattcatt aatgtatcaa taatgacgaa ggcattctga tgcaacatta
    11401 cgtcgatctc ttcttcgttc tgtatatgaa cgacattacc tttactcaga atgtttttca
    11461 tcgcggtgaa gaacatcgta tccctggtaa ttataagaaa cataaccgac tcctggtgcg
    11521 gacattaact ttataatcag tttgccaaag gtaattaaca ggacagggct tataataata
    11581 atagttagct tactgtatat taattttatg taatacatga tgcagatgct gagaaagcat
    11641 ctgcatcttt cggtggtgtt atttgaccag ctcaggtgtt acctgactca ccgcattggt
    11701 gtagtaaggc gcaccccagc ccggttccgg ccagaatgca ccatagccgt aatcccacag
    11761 gttataggtg gtattaacca cttctctcaa atgccacacc tggatgccct gcatattcac
    11821 ttccaggaag ttatacggga ctttattgat aaagcctggt tgttcaccct gaatggtacc
    11881 gaggatggta acaaagtgat tacgatagtt cactggatca aggaagccgc tttgtctggc
    11941 gagcagtcgg ccctgatagt tggcttcaat atcaggcttc gcatagctat ccaacggtaa
    12001 tacagcgatt tctaacaacg tatccgtttt gccattgata acgttgataa ccttcccacc
    12061 aaagcgcgct tgttgaccaa catataaccc cggctggtta tgaacagcaa caaaactttt
    12121 ttgaatatca ggttggttat tgcctttgat attttgcgga attgaactac atgcggcaag
    12181 caaaaatgaa aggctgagga tgagtgcacc ttttgtcatg ttcatgttac tatccttatc
    12241 aacaaatatt aaaaccataa tagatatttc tttatagttt tcatctgatt ctgaggtgat
    12301 atgcctgata gtaacgtaaa cctgtctgat gtttatatcc cttctgttta gttcaattcc
    12361 gttattttgg ggcgtggtat gagtatccat cgcccaataa tataaccctt tgttttaaaa
    12421 ttacataaaa tctattttta ttcaataatt ataaagtgct aataatcaat attatggtat
    12481 tatattctga taatgatttg ataagcgaat tcttttgcca gatggtattt ctatttccac
    12541 cccataattt atatcttttt cattaggaag tggcctcttt aaataaggcg tgtgtgacat
    12601 ttttttgggg ttaagcgtct cgtattgaaa tacttcgaac tgatattcag acatttctgc
    12661 ccatgtttga attttattgt atcgagagag tgtcgcaaag gctttaactt gcttataaca
    12721 ttgtttttgg aattcttgct gatatgttaa tatgtcattg tatgaaagat tggttatcct
    12781 agcctctaaa aatttaatta ttgtcgaagc aatttccgct tttgctcgta aaaagttgtc
    12841 attaatggtt aataatgttg aaaataaacc ttcgatttgt ttgtcaagat attgtccatc
    12901 tttatataat ttgggggtag ggtgttcttt atgtaaaaaa tagtgtcccc taaatttgcg
    12961 tcagtgaaat ttatattaag tagatcttcg aaataaaacc ttgacaaggt tagaacgaac
    13021 ccggacaggt tctgtcctgt catattgaaa tcaataaagt tggttccggc aaagactttc
    13081 atcctgtttt tattttcact gtgctcaata tataacttcg tcattttttg atacaggctt
    13141 tgaatatctt ttatattagt tggtaagtca gctcgttgtt cagcaataaa attagtatct
    13201 gaaaacatgg tttggacact gaatgttatg ggtattccct cttctacgct aattttagtt
    13261 atgattgcct tgtcgggttt aggattagcg acaacaatga ttcgacattc agcacttttg
    13321 tttttttcat atctaatgtt attaatctga ttaagtattg attgtgtctg ttgcgtaagt
    13381 ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac cacaactttt
    13441 gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg ttggagttgt
    13501 tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatat ggaattaatg
    13561 ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt tttaacatta
    13621 tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat acctggggta
    13681 aagtcaattg acataacatt ccttattcat catctgtt
//
LOCUS       DAZGAN020000127        13640 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGAN020000127 DAZGAN020000000
VERSION     DAZGAN020000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.127"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..937
                     /locus_tag="ACHKYO_RS20580"
                     /old_locus_tag="pgaptmp_004116"
     CDS             <1..937
                     /locus_tag="ACHKYO_RS20580"
                     /old_locus_tag="pgaptmp_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HGU4430876.1"
                     /translation="IPVHDTIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGK
                     TLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELLNMLDIKGKII
                     TTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSE
                     KSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYI
                     SSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINI
                     LTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(979..1749)
                     /gene="yafV"
                     /locus_tag="ACHKYO_RS20585"
                     /old_locus_tag="pgaptmp_004117"
     CDS             complement(979..1749)
                     /gene="yafV"
                     /locus_tag="ACHKYO_RS20585"
                     /old_locus_tag="pgaptmp_004117"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HGU4430877.1"
                     /translation="MPGLKITLLQQPLVWMNGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRLR"
     gene            1930..2376
                     /gene="ivy"
                     /locus_tag="ACHKYO_RS20590"
                     /old_locus_tag="pgaptmp_004118"
     CDS             1930..2376
                     /gene="ivy"
                     /locus_tag="ACHKYO_RS20590"
                     /old_locus_tag="pgaptmp_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HGU4430878.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2419..4863)
                     /gene="fadE"
                     /locus_tag="ACHKYO_RS20595"
                     /old_locus_tag="pgaptmp_004119"
     CDS             complement(2419..4863)
                     /gene="fadE"
                     /locus_tag="ACHKYO_RS20595"
                     /old_locus_tag="pgaptmp_004119"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HGU4430879.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            5103..5681
                     /gene="lpcA"
                     /locus_tag="ACHKYO_RS20600"
                     /old_locus_tag="pgaptmp_004120"
     CDS             5103..5681
                     /gene="lpcA"
                     /locus_tag="ACHKYO_RS20600"
                     /old_locus_tag="pgaptmp_004120"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HGU4430880.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5887..6654
                     /gene="yafJ"
                     /locus_tag="ACHKYO_RS20605"
                     /old_locus_tag="pgaptmp_004121"
     CDS             5887..6654
                     /gene="yafJ"
                     /locus_tag="ACHKYO_RS20605"
                     /old_locus_tag="pgaptmp_004121"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HGU4430881.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6625..7365)
                     /gene="dpaA"
                     /locus_tag="ACHKYO_RS20610"
                     /old_locus_tag="pgaptmp_004122"
     CDS             complement(6625..7365)
                     /gene="dpaA"
                     /locus_tag="ACHKYO_RS20610"
                     /old_locus_tag="pgaptmp_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HGU4430882.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPNSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(7521..7799)
                     /gene="yafQ"
                     /locus_tag="ACHKYO_RS20615"
                     /old_locus_tag="pgaptmp_004123"
     CDS             complement(7521..7799)
                     /gene="yafQ"
                     /locus_tag="ACHKYO_RS20615"
                     /old_locus_tag="pgaptmp_004123"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HGU4430883.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(7802..8062)
                     /gene="dinJ"
                     /locus_tag="ACHKYO_RS20620"
                     /old_locus_tag="pgaptmp_004124"
     CDS             complement(7802..8062)
                     /gene="dinJ"
                     /locus_tag="ACHKYO_RS20620"
                     /old_locus_tag="pgaptmp_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HGU4430884.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            8248..9021
                     /gene="yafL"
                     /locus_tag="ACHKYO_RS20625"
                     /old_locus_tag="pgaptmp_004125"
     CDS             8248..9021
                     /gene="yafL"
                     /locus_tag="ACHKYO_RS20625"
                     /old_locus_tag="pgaptmp_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4430885.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGEIIRVSQLAEPFWQDHFLGARRILTEETIL"
     gene            complement(9078..9434)
                     /locus_tag="ACHKYO_RS20630"
                     /old_locus_tag="pgaptmp_004126"
     CDS             complement(9078..9434)
                     /locus_tag="ACHKYO_RS20630"
                     /old_locus_tag="pgaptmp_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGU4430886.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(9427..9705)
                     /locus_tag="ACHKYO_RS20635"
                     /old_locus_tag="pgaptmp_004127"
     CDS             complement(9427..9705)
                     /locus_tag="ACHKYO_RS20635"
                     /old_locus_tag="pgaptmp_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGU4430887.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            complement(9810..11522)
                     /locus_tag="ACHKYO_RS20640"
                     /old_locus_tag="pgaptmp_004128"
                     /pseudo
     CDS             complement(9810..11522)
                     /locus_tag="ACHKYO_RS20640"
                     /old_locus_tag="pgaptmp_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            11494..12279
                     /gene="lafU"
                     /locus_tag="ACHKYO_RS20645"
                     /old_locus_tag="pgaptmp_004129"
     CDS             11494..12279
                     /gene="lafU"
                     /locus_tag="ACHKYO_RS20645"
                     /old_locus_tag="pgaptmp_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HGU4430888.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFSGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            12350..13405
                     /gene="dinB"
                     /locus_tag="ACHKYO_RS20650"
                     /old_locus_tag="pgaptmp_004130"
     CDS             12350..13405
                     /gene="dinB"
                     /locus_tag="ACHKYO_RS20650"
                     /old_locus_tag="pgaptmp_004130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGU4430889.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELHLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            13402..>13640
                     /locus_tag="ACHKYO_RS20655"
                     /old_locus_tag="pgaptmp_004131"
     CDS             13402..>13640
                     /locus_tag="ACHKYO_RS20655"
                     /old_locus_tag="pgaptmp_004131"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4430890.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYID"
BASE COUNT         3379 a         3557 c         3464 g         3240 t
ORIGIN      
        1 tattcctgtt cacgatacta ttgccagagt tgtatcctgt atcagtcctg caaaatttca
       61 tgagtgcttt attaactgga tgcgtgactg ccattcttca gatgataaag acgtcatcgc
      121 aattgatgga aaaacgctcc ggcactctta tgacaagagt cgccgcaggg gagcgattca
      181 tgtcattagt gcgttctcaa caatgcacag tctggtcatc ggacagatca ggacggatga
      241 gaaatctaat gagattacag ctatcccaga acttcttaac atgctggata ttaaaggaaa
      301 aatcatcaca actgatgcga tgggttgcca gaaagatatt gcagagaaga tacaaaaaca
      361 gggaggtgat tatttattcg cggtaaaagg aaaccagggg cggctaaata aagcctttga
      421 ggaaaaattt ccgctgaaag aattaaataa tccagagcat gacagttacg caatgagtga
      481 aaagagtcac ggcagagaag aaatccgtct tcatattgtt tgcgatgtcc ctgatgaact
      541 tattgatttc acgtttgaat ggaaaggact gaagaaatta tgcgtggcag tctcctttcg
      601 gtcaataata gcagaacaaa agaaagagcc agaaatgacg gtcagatatt atatcagttc
      661 tgctgattta accgcagaaa agttcgccac agcaatccga aaccactggc acgtggagaa
      721 taagctgcac tggcgtctgg acgtggtaat gaatgaagac gactgcaaaa taagaagagg
      781 aaacgccgca gaattatttt cagggatacg gcacatcgct attaatattt tgacgaatga
      841 taaggtattc aaggcagggt taagacgtaa gatgcgaaaa gcagctatgg acagaaacta
      901 tctggcgtca gtccttgcgg agagcgggct ttcgtagtct taccccgact ctcccccagc
      961 cttaaacaca acccccactc accgcaacct aaactcatcc gcatcccgcc atgccggaaa
     1021 cttatcgcga tattccctta acaccgccat cgacagctcg gcatcaatgc gcgttgcctg
     1081 atgcgcgtcg gcagtagcga taatctcgcc ttgtggatta atcacccggc tgtcaccgcg
     1141 ataatggcag ccgttgccat cgctgccgac gcgattgcat cccgccacat acgcctgatt
     1201 ctcaatcgcg cgggccgtca gcaatgcctg ccagtgcaga gagcgcggag caggccagtt
     1261 ggcgacgtac agggcgaggt cataatcgtt gagattgcgc gaccacacag gaaaacgtaa
     1321 gtcgtagcac accagcggca aaatacgcca gccgcgccat tccacaatca ctcgcgcatt
     1381 gcccgcttta taatgtagat gctcatctgc catgcggaac agatgacgct tatcataaaa
     1441 atgtaccgtg ccgcccggct caaccagcaa aaagcggtta accgaaccag actccgtttg
     1501 taatgcaaca ctgcctgcaa tcagcgcatt gcactgctgc gccttagctg tcatccagtt
     1561 cactacgtca tcttgtgcta gcgacgaagc tgccgcttcc atggcaaagc cgctggtaaa
     1621 catctccggt agaacgatca catcgcgccc ggtaatacct tccagttgac gatcaaaatg
     1681 acgcaggttg gcagggccat tcatccacac cagtggttgc tgcaaaagcg taatcttcaa
     1741 accaggcacg gtgtacaact cctttatgcg aagggtttta taactttaac accttatcag
     1801 gcagttgcct tagcgcagaa taaattgata acaaatgctg atattggaaa tatctgattt
     1861 gcaaattatc gtgttatcgc caggctttag aaggttaata acatgggcag gataagctcg
     1921 ggaggaatga tgtttaaggc aataacgaca gtcgccgctc tggtcatcgc caccagtgca
     1981 atggcgcagg atgatttaac cattagcagc cttgcaaagg gcgaaaccac caaagctgca
     2041 tttaatcaga tggtacaagg gcataagctg cctgcctggg tgatgaaagg cggtacttat
     2101 actcccgcac aaaccgtaac gttgggagat gagacgtatc aggtgatgag cgcgtgcaaa
     2161 ccgcatgact gtggctcgca acgtatcgct gtgatgtggt ccgagaaatc taatcagatg
     2221 acggggctgt tctcgactat tgatgagaaa acgtcgcaag agaaactcac ctggctgaat
     2281 gtgaacgatg cgctttcgat tgatggtaaa acggtgctgt tcgcggcgtt gaccggcagc
     2341 ctggaaaacc atccggatgg ctttaatttt aaataattag cggataaaga aacggagcct
     2401 ttcggctccg ttattcaatt acgcggcttc aactttccgc actttctccg gcaactttac
     2461 cggcttcgtc gccagctctt ccggatcaaa gtcatcaacg ttaatactgc gcagacggct
     2521 ttcttcagct ttcaccagaa tagcggcttc atctttatca atcagcccct tcgccagcgc
     2581 gttgtgcgcc agttcatcca gacgggtaaa cggcaggttt ttacccagct ctttacagat
     2641 ccgctgatga attgggtcgg cggcaatcac atccaccagc gcctcttcca gcaagccaac
     2701 cggattatgc tcgctcggcg tcaggtactg accgcgacca atgcgggaac gggtggcgtt
     2761 cggcacttgt aaaatcttcg ccactttatg atccagcttg tcagaaggtg ccagataatg
     2821 acgtccggtc gggaagatca ccacattcag cagcccggca accacgcggt tcgggaagtt
     2881 ttgcagtaaa tcatccatcg cctgttcagc ctgatacagc gcatcttgta cgccccagtg
     2941 caccagcggc aggtcggctt cattacggcc ttcgtcgtca taacgcttca gaacggcaga
     3001 ggcgaggtaa agctggctta aaatatcccc cagacgtgcc gagatgcgct cacgacgttt
     3061 caggctgccg cccagcacag ccatcgagac atcagaaagc agggcgaggt tggcgctcag
     3121 gcggttcagg tgctgatagt agcgtttagt ggcatcgccg gttggcgtgc tgctggttaa
     3181 accgcgcgtc aggcccagcc agaagctgcg aactttgttg ctaccgacgt gaccgatatg
     3241 tttgaacaac agtttatcga acgcgttgac gtcattgttc ttcgctgctt ccatctcttc
     3301 cagcacgtac ggatggcaac gaatcgctcc ttgtccgaag atcatcatgc tgcgggtcag
     3361 aatgttggcc ccttcaacgg tgatggcaat cggtgcgccc tggtaagcac gcgccaggaa
     3421 gttgctctgc ccgagcataa tgcctttgcc accagtgata tccatcgcat caataatcga
     3481 ctgctgcccg cggtgggtac agtgatactt aacgatagcc gacagcacgg caggtttttc
     3541 gccgagcata atgccgtagg taatcagcga tgccgcagca tccatcacgt aggcattacc
     3601 ggcaatacgc gccagcggct cttcaatccc ttccatctta ccgatagaga ttttgaactg
     3661 acggcgaatg tgagcatacg cgcccgttgc cagcgctact gatttcacgc cgccggttga
     3721 gttggaaggc agggtgatgc cgcggcctac cgagaggcac tccaccagca tccgccagcc
     3781 ttgcccggcc attttcggcc cgccgataat gtaatcgatc ggtacgaaga catctttacc
     3841 gcgcgtcggt ccattctgga acggcacgtt cagcgggaag tggcgacgac caatttccac
     3901 gcccggcgtg gtggttggga tcagcgcaca ggtaatacct aaatcttctg cgccacccag
     3961 caatttctcc gggtcggaga gtttaaacgc cagcccaagc acggtcgcaa tcggtgccag
     4021 cgtaatgtag cgtttgttcc aggtcagacg catccccagc acctgctggc cctgccattc
     4081 gcccatgcag acaatcccgg tatccgggat agcgcctgca tcggaacccg cttccgggct
     4141 ggtcagtgca aagcaaggga tctcctgacc acgcgccaga cgcggcagat agtgattttt
     4201 ctgctcgtca gtgccgtaat gttgcagcag ctcgcccggg cctaatgagt tcggcacgcc
     4261 gacggtaatc gccaggatcc cgctcacgcc ggagagtttt tgcagcacgc gagactgggc
     4321 ataagccgag aactccagcc cgccgtactc ttttttgatg atcatcgcga agaaacgatg
     4381 ctctttaagg tacgcccaca actccggcgg caaatcagcc agctcatggg tgatctggaa
     4441 atcattcgcc atccggcagg cttcttctac cggaccgtcg agaaacgctt gctcttctgc
     4501 ggtcaggcgc ggctgcggat agttatgcag ctttttccag tccggcttgc cctggaacaa
     4561 gtcgccctcc caccaggtgg tgcccgcatc aatcgcttct ttctcagtgc gcgacatcgg
     4621 cggcatcacc ttacggaaac cgcgaaatac cggcgcggaa atcatcgact tacgcatagg
     4681 cgcaaagtta aatggcacga ggataatggc cagaggcacc agtacccacg ccgaccacag
     4741 accagcaacg ccgagggcgg ctgtccaggc gagcaaaatc agactgctga taaataagct
     4801 cacgcggtga tagaacaacg cgccgagcag gacaaccgta gcgagaatac tcaaaatcat
     4861 cataacgaaa agccccttac ttgtaggagg tctgaccact tgtgatgata tggttgtagt
     4921 ggatgtaaaa acatttagca atgtgtttac aatataatta caacaaagct cacattgttg
     4981 ctgtttttat ccgcacttca ggtcaaaaag tcctggtcat agcacctgcc cgtacttctc
     5041 gcttttggcg gtatccggta cactgcattt tgtctattac atttatgctg aaggatatcc
     5101 tcatgtacca ggatcttatt cgtaacgaac tgaacgaagc ggcggaaacg ctggctaact
     5161 ttttaaaaga tgacgccaat attcacgcca ttcagcgcgc ggcggtcctg ttagcagaca
     5221 gctttaaagc cggtggcaaa gtgctttcct gcggcaacgg cggttcccat tgcgacgcta
     5281 tgcactttgc cgaagagttg accggtcgct accgtgaaaa ccgtccgggc tacccggcga
     5341 ttgctatttc tgacgttagt catatttcat gcgtcggtaa cgatttcggt ttcaatgata
     5401 ttttctcccg ttacgttgaa gcggtaggtc gcgaaggcga tgtactgctg gggatctcca
     5461 cctccggtaa ctctgcaaac gtgatcaaag cgatcgcagc ggcgcgtgag aaggggatga
     5521 aagtgatcac cctgaccggt aaagacggcg gcaaaatggc tggcacggcg gatatcgaaa
     5581 ttcgcgtacc gcactttggt tatgccgacc gcattcagga gattcacatt aaagtgatcc
     5641 atatcctgat ccagttaatt gaaaaagaga tggttaagta agtctggcgt aggctggata
     5701 aggcgtttac gccgcatccg gcacttgtgc gctgatgcct gatgcgatgc tgacgcgtct
     5761 tatcatgcct acaaatctgt acccgaaccg taggccgaat aattcgttta cgccgcatcc
     5821 gacctgaaaa ttcttaaatc aatcttcgcc gggggccatt cgctcccgct gttgtggagg
     5881 ttacccatgt gcgaattgct cgggatgagc gccaacgtcc ctaccgatat ctgctttagt
     5941 ttcaccggac ttgtacagcg tggtggtgga accgggccac ataaagatgg ctggggcatt
     6001 accttttacg aaggtaaagg ctgtcgcaca tttaaagatc cacaacccag ctttaattcc
     6061 cccatcgcca aacttgtcca ggactacccg ataaaatcct gttcggtggt ggctcatatt
     6121 cgccaggcta atcggggcga ggtggcgctg gaaaatactc acccatttac ccgcgagtta
     6181 tgggggcgta actggactta tgcccataac ggacaactga cgggctacaa atcactggaa
     6241 accggcaact tccgcccggt cggtgaaacc gatagcgaaa aagccttctg ctggctcctg
     6301 cataaattaa cgcagcgtta cccgcgcaca ccgggcaaca tggcggcagt gtttaaatat
     6361 atcgcctcac tggcggatga actgcggcag aagggcgttt tcaacatgct gctttctgac
     6421 gggcgctatg tgatggcgta ttgctcgact aatttacact ggatcacccg ccgcgcgccg
     6481 tttggcgtgg caacgctgct ggatcaggat gtggaaatcg acttcagctc gcagaccaca
     6541 ccgaatgatg tggtcacggt gattgcaaca cagccgctga cgggcaatga aacctggcaa
     6601 aagattatgc caggcgaatg gcgcttattt tgcctcgggg agcgtgtagt ttgatgccag
     6661 ttgtggctgc acaacttcgt ggcttaacgg cttgctgacc acgtaacgac cattgaccac
     6721 agaaacggtc ggtggcttac gggtttgctc aaagtagtcg taacccggct tcagttgctc
     6781 ccagaagtcc ttaaagttgg aatatttatg gcgcttcata ttggcgtcgg tcatgcggaa
     6841 cgggtaaata ctcacctgca cgctcggctg accaaacacc agcgcaccag taacgaactg
     6901 gaagatctca tcaataccct gattggtcat tgcgtagcag ccgatggaaa cacaatcgcc
     6961 gtggatcatc aggtatttcc cttcataacc atgcgcacgg tcataggcat tggggaaacc
     7021 aatattaatc gctttgtagt aacggctgtt tggttttaac tgattacgct ggacgctata
     7081 aaacccttcc gggcttttga aatcgccctg acgctgtttt ggccctaagc cgccggaata
     7141 tttacagatt ttataactgt cgagcagctg atattgctcg cccattttga cgtagagatc
     7201 gagcgtacgt tcttccttga agatctggat gtagacaggg gatcccatca actgctgctt
     7261 atactctttg ctcactggcg tggtcgagct actgctgccc agcaaaccgg caaacgaaac
     7321 gcacgggatc aacaacatcg caagaattaa tgcgatttta cgcatactgc ttattccttg
     7381 ataaaacggt tacacacgcc aggacggcaa aatagatccc aaatcggaat agtctggatt
     7441 tggaaggctc acattatcac caaaagagtt ttacgcaagc ctgtcggcgc agggtttaca
     7501 aatttcatcg gcagcgtgct ttacccaaag agcgccgcgt gagttccagt tctctcaaat
     7561 cgtaaaagtt tatcggtaag tttgtaaatc aggatccagt ccggttcgac atgagcatcg
     7621 cgataacctt tccatgaacc ttgcagcggg tggtctttat aaacagctgg aagcggtaaa
     7681 gcattattga taagaagcgt cataagatat ttcaatttat tcatatcctt atgacgcttt
     7741 tgtgcaagtt ttacatcctt tgaaaattgt cccgagtatt caatatccct ttgaatcata
     7801 tttaaattcc taatttatca aataaatcat cggcgtcttt ggccttatga acatcaacgc
     7861 cagcttcgct gtttttgatt gattgaatgg ttaattgatt aggctcgcgt aaatcaaacg
     7921 gcaatgcctt ttcacgcgcg acctttgtga gggttatgcg aaccaggtca gagatggtca
     7981 gccccatccc ggccagtacg tccgccgcct gattcttcag atcttcatcg attcgggcgc
     8041 gaacaaatgc gttagcagcc attggggtat ctcctgtgct gtatatttat tcagcttgaa
     8101 ttgtagctca aatgagcaac agcgacaatc catgagtaat ttaataaacg gatgttttta
     8161 cataattaac tgaaatatat ttattaattt tataataatg gtttattaag tttgttcagg
     8221 tacgggccgg gatacactgc aacatcaatg catgatcagc cattattgag tatgtccttg
     8281 ccgtcgattc cctcttttgt gttgtcggga ttactgttgc tttgcttgcc gttttcttca
     8341 tttgccagcg ccaccacatc acatatcact ttcagctacg ccgcccgcca gcggatgcaa
     8401 aatcgtgcgc gtttattaaa acagtaccaa acccatctga aaaaacaggc cagctatatt
     8461 gtggaaggca atgccgaaag cagaagggcg ctacgccagc acaaccgcga acagataaaa
     8521 cagcatccag aatggtttcc tgcgccgctc aaggcgagtg acagacgctg gcaggcgctg
     8581 gcggaaaaca accatttctt aagcagcgac catctgcata acatcaccga agtggcgatt
     8641 caccgtctgg agcaacagct tggcaagcct tacgtctggg gcggtacgcg gcccgatcaa
     8701 ggcttcgact gtagcgggtt agttttttat gcctacaaca agatccttga ggcgaaactc
     8761 ccacgcacgg ctaatgagat gtatcactat cgccgggcaa ccattgtggc gaataacgac
     8821 ctgcgccggg gagatttgct atttttccat atccacagcc gcgagattgc cgaccatatg
     8881 ggggtgtatc tgggcgacgg tcagtttatt gagtcaccgc gcaccgggga aatcattcgg
     8941 gtaagtcagt tagctgaacc tttctggcag gatcactttt taggcgcgcg gcggattttg
     9001 acggaggaga cgattttgta aatgggtaat attgacgaac tggcaaaagg tcggatgcca
     9061 cgcggcatcc gacctgttta catctgggct gaaaactcac gttctaagac atcaacaatg
     9121 agctggtttt ttgaaatgga atgttgtctg gcgacgatgg tcagccgcga atcaatgtga
     9181 tgaggaacac gaagcgtcag gcgtttttgc tcttcttctt cacgaaaagg ggcaataccg
     9241 tcctcttcac aatccgccat ccactcatcc agcgcgcttt cgccttcacg atataatgcc
     9301 tcaatacttg tggctacaaa gtcacaataa ccgtttacat tgaggaactt gccgcgaaat
     9361 gcgttgatat cggcttcaaa tgttactgcg gctggttgac ctttaattgt gataatgttc
     9421 gatgctttat tcataatcta cccctacaca gttaacaaac cactccttaa gacctgctac
     9481 tgcgccttta tccatcacat tttttggatg tggctcatgt gtgctgaatt tggtcgttcc
     9541 aatctgaaat tttcgtctgg agccgttgtt attttttatt gttccaccca cagctttaat
     9601 gagtgcaaca acatcatccc atttaattcc agattttggc ggctccgtca ttacatcgtc
     9661 gaatgttttt ctatgacgag aacctagcgc gctgacctta atcatactat caatccatga
     9721 cgtcattgta tggtgtcatc gtagtctctt tgcagtgtga cgtcaattaa tggtgtcagg
     9781 tgctgcaaag catggaaaag aatcccccat caactcaacg cccccataat cttcaactcc
     9841 agctcatccg gcacttcgtt atacgacagc acatgcagcc ccggcgcaaa caaccttgca
     9901 taacgcgcca gcaaagggcg cagctgcggt ggcaccagca gcaccgggtc tttccccgcc
     9961 gctttcatct gctctttcac ctgtggcatc gtactctgga actggttgag catattcggg
    10021 tccactggca cgctgtcgag catcacttta ccgccctgtt gcgcctgatt cactaggtta
    10081 gtcagcagat tttccagctc attattcagg gtatacaccg tcagctcctg cttgcgaacg
    10141 aacggatggg taatgctgcg ccgcagcgcc aggcgcacat cggctgccag cagaatatga
    10201 tctttggtca ccgcgctact ggcgaccagc acggtggcga tggtgacgat atcgcgcagg
    10261 gaaacgcctt cggtcagcag cgcccggtac actttcagca actggctgta attgagcacc
    10321 gcgcttaaat cctccgccag gcgcggtgcc atcgacgcca aacggttatg caactgcgta
    10381 atatcgtcat agttaaataa atcaggaata tagctgcgca caatcttatt tacatgggta
    10441 gcaatcacgc tggcgctgtc gatcacctga taccccatat tcagcgcctt cgctttctgc
    10501 gcaggctgga tccaggtcac cggcatcccg tacgccgggt cgttccccag cacgccgtct
    10561 atctcgccgt aggtttcgct ggagggcagc gccatcagtt tatccgccgg aatatccgct
    10621 tcatcagcct taatgccgtt gatgaaaata gcgtactggc tgggcttaag gcggaagttt
    10681 tcccgaatgc ggatctccgg cagcagcacg ccgttgccgt cggatatcac ctgtcgcacg
    10741 ccgcgaatac gctgggtgag cgggttgccc tgggctttgt ccaccagcgc caccagcttg
    10801 tagccgaggc ttaagctgat gggctcgatc agcggaatgg tttcccaact gacctgttgc
    10861 tcgctggttt cggtgatggt gcgggtcagc gtttcgagac ttttctcttc cgcttccgcc
    10921 gcctgcgggc gtttgctcat ccgccagccg gtaaagccaa gcagggcgct gaacagcaaa
    10981 aacggcagat gtggcattcc aggcactacc gccagtacga acataattcc ggtagcggtg
    11041 tagagcaccg atgggcttgc cagcaactgg tgacgcacgt catgggcgat atcgccgttg
    11101 tcgctgacgc gggtgacgat aatcgccgcc gcggtggaga gtagcaggga agggatttgc
    11161 gccaccagcc cgtcgccgat ggtcatcagc acatactgct ggaaggcggc gtcggcgctc
    11221 aggttgtatt tgaagatccc gatacagacg ccgccgatca ggttgatcgc cagaatcatc
    11281 atcccggcga tagcatcccc gcgcacaaat ttcgatgccc cgtccatcgc gccgtagaaa
    11341 tcggcctcgc tggcaacatc tttacgtcgg gtttgtgcct gcgcctgatt gatcaacccg
    11401 gcattaagat cggcgtcaat cgccatctgc ttaccgggca tcgcatccag ggtaaagcgg
    11461 gctgaaacct cggaaatacg ctcggcccct ttggtgacga caataaagtt gattgtcaac
    11521 agcgtcagta aatccgagcg tgaaagcatt atcgccgcgc tgcacgggca gtcgattttc
    11581 agtggcggcg gattgtcgcc gttaaataaa atcagcccgt ctcatccgcc aaaacctgcc
    11641 accgtggcgg tgccggaaga gacggagaaa aaagcgcgag acgtcaacga gaaaacagcg
    11701 ctgctgaaga agaaatccgc caccgagctt ggcgagctgg caaccagtat caacaccatt
    11761 gcccgtgatg cgcatatgga agcgaatctg gagatggaga ttgttcccca gggattgcgc
    11821 gtgctgatta aagatgacca gaaccgcaat atgttcgaac gcggcagcgc gcagattatg
    11881 ccgttcttta aaactttgct ggtggagctc gcgccagtgt tcgactcgct cgataacaaa
    11941 attattatta ccgggcatac cgatgcgatg gcctacaaaa acaatatcta caacaactgg
    12001 aacctttcgg gcgatcgcgc gctttcagct cgtcgggtgc tggaagaggc cggaatgccg
    12061 gaagataaag tgatgcaggt aagcgcaatg gcggaccaga tgctgctgga tgccaaaaat
    12121 ccgcaaagcg cgggcaatcg gcgtattgag attatggtgc tgaccaaaag tgcgtccgat
    12181 acgctgtatc aatactttgg tcagcatggg gataaagtgg tgcagccgct ggtgcaaaag
    12241 ctggataagc agcaggtgct ttcgcagcga atgcgctaaa tgctgaatct ttacgcattt
    12301 ctcaaaccct gaaatcactg tatactttac cagtattgag aggtgagcaa tgcgtaaaat
    12361 cattcatgtg gatatggact gctttttcgc cgcagtggag atgcgcgaca atcccgccct
    12421 gcgcgatatc cctattgcta ttggcggcag ccgcgaacgt cggggggtga tcagcaccgc
    12481 caattatccc gcgcgtaaat ttggcgtacg tagcgctatg cctactggga tggcgctcaa
    12541 attatgccca catctcacct tgcttccggg gcgctttgac gcctacaaag aagcctcaaa
    12601 tcatatccgt gaaatcttct cgcgctacac ctcgcgcatt gaaccgttgt cactggatga
    12661 ggcttatctc gatgtcaccg atagcgtcca ttgccacggt tctgcgaccc tcatcgccca
    12721 ggaaatccgc cagacgattt tcaacgagct gcacctgacg gcgtctgcgg gcgttgcgcc
    12781 ggtcaagttt ctcgccaaaa tcgcctccga catgaataaa cccaacggcc agtttgtgat
    12841 tactccggca gaagttccgg catttctgca aaccttacca ttggcaaaaa tccccggcgt
    12901 cggcaaagtc tcggcggcaa aactggaagc gatggggctg cggacctgcg gtgatgtgca
    12961 aaagtgtgat ctggtgattc tgcttaaacg ctttggcaaa tttggccgca ttttgtggga
    13021 gcgtagtcag gggattgacg agcgcgacgt taacagcgaa cggttgcgaa aatccgtcgg
    13081 cgtggaacgc acgatggcgg aagatatcca ccactggtct gaatgtgaag cgattatcga
    13141 gcggctgtat ccggaacttg aacgccgtct ggcaaaggta aaacctgatt tactgattgc
    13201 ccgccagggg gtgaaattaa agttcgacga ttttcagcaa accactcagg agcacgtctg
    13261 gccgcggctg aataaagctg acttaatcgc caccgcgcgt aaaacctggg atgaacgccg
    13321 cggcgggcgc ggtgtgcgac tggtggggct gcatgtgacg ttgcttgacc cgcaaatgga
    13381 aagacaactg gtgctgggat tatgaataac atacaaataa gagactatca gcctggcgat
    13441 tttcagcaac tatgcgctat tttcattaga gcggttacga tgactgccag tcagcattat
    13501 tcaccacaac aaattgccgc ctgggcgcag attgacgaat ctcgctggaa ggagaaactc
    13561 gcgaaatcac aagtgcgggt tgcagtcatt aatgcaaaac tggtcggctt tattacctgc
    13621 gttgaacact atatcgatat
//
LOCUS       DAZGAN020000128        13411 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGAN020000128 DAZGAN020000000
VERSION     DAZGAN020000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.128"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..341
                     /locus_tag="ACHKYO_RS20660"
                     /old_locus_tag="pgaptmp_004132"
     CDS             <1..341
                     /locus_tag="ACHKYO_RS20660"
                     /old_locus_tag="pgaptmp_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGU4430891.1"
                     /translation="TMQLMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHY
                     PIKKAYTPYNIHTYKTEEVVNQRNIKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLD
                     RSFSSSSWPF"
     gene            665..2053
                     /gene="narU"
                     /locus_tag="ACHKYO_RS20665"
                     /old_locus_tag="pgaptmp_004133"
     CDS             665..2053
                     /gene="narU"
                     /locus_tag="ACHKYO_RS20665"
                     /old_locus_tag="pgaptmp_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HGU4430892.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALCFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2135..5875
                     /gene="narZ"
                     /locus_tag="ACHKYO_RS20670"
                     /old_locus_tag="pgaptmp_004134"
     CDS             2135..5875
                     /gene="narZ"
                     /locus_tag="ACHKYO_RS20670"
                     /old_locus_tag="pgaptmp_004134"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HGU4430893.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYTAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5872..7416
                     /gene="narH"
                     /locus_tag="ACHKYO_RS20675"
                     /old_locus_tag="pgaptmp_004135"
     CDS             5872..7416
                     /gene="narH"
                     /locus_tag="ACHKYO_RS20675"
                     /old_locus_tag="pgaptmp_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU4430894.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7416..8111
                     /gene="narW"
                     /locus_tag="ACHKYO_RS20680"
                     /old_locus_tag="pgaptmp_004136"
     CDS             7416..8111
                     /gene="narW"
                     /locus_tag="ACHKYO_RS20680"
                     /old_locus_tag="pgaptmp_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU4430895.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQRKAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGEK"
     gene            8108..8788
                     /gene="narI"
                     /locus_tag="ACHKYO_RS20685"
                     /old_locus_tag="pgaptmp_004137"
     CDS             8108..8788
                     /gene="narI"
                     /locus_tag="ACHKYO_RS20685"
                     /old_locus_tag="pgaptmp_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU4430896.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8867..9760
                     /gene="yddE"
                     /locus_tag="ACHKYO_RS20690"
                     /old_locus_tag="pgaptmp_004138"
     CDS             8867..9760
                     /gene="yddE"
                     /locus_tag="ACHKYO_RS20690"
                     /old_locus_tag="pgaptmp_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HGU4430897.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9856..10701)
                     /gene="nhoA"
                     /locus_tag="ACHKYO_RS20695"
                     /old_locus_tag="pgaptmp_004139"
     CDS             complement(9856..10701)
                     /gene="nhoA"
                     /locus_tag="ACHKYO_RS20695"
                     /old_locus_tag="pgaptmp_004139"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HGU4430898.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10874..11443
                     /gene="yddH"
                     /locus_tag="ACHKYO_RS20700"
                     /old_locus_tag="pgaptmp_004140"
     CDS             10874..11443
                     /gene="yddH"
                     /locus_tag="ACHKYO_RS20700"
                     /old_locus_tag="pgaptmp_004140"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HGU4430899.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11447..11674)
                     /gene="pptA"
                     /locus_tag="ACHKYO_RS20705"
                     /old_locus_tag="pgaptmp_004141"
     CDS             complement(11447..11674)
                     /gene="pptA"
                     /locus_tag="ACHKYO_RS20705"
                     /old_locus_tag="pgaptmp_004141"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HGU4430900.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11773..12909)
                     /gene="ydcC"
                     /locus_tag="ACHKYO_RS20710"
                     /old_locus_tag="pgaptmp_004142"
                     /pseudo
     CDS             complement(11773..12909)
                     /gene="ydcC"
                     /locus_tag="ACHKYO_RS20710"
                     /old_locus_tag="pgaptmp_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            13276..13395
                     /locus_tag="ACHKYO_RS20715"
                     /old_locus_tag="pgaptmp_004143"
                     /pseudo
     CDS             13276..13395
                     /locus_tag="ACHKYO_RS20715"
                     /old_locus_tag="pgaptmp_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         3240 a         3425 c         3355 g         3391 t
ORIGIN      
        1 ctactatgca gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc
       61 tggcaattct ttcgcctggt gcatttgaag ttcactctga cggaatagat gtggataacg
      121 aattgtttca ctatcctatt aaaaaagcat ataccccata taatatacac acttacaaga
      181 cagaggaagt tgtaaaccag aggaatataa aagttaaaaa catgacctta gatgaaataa
      241 acaatactta ctgtaataac gattattaca atcaggcaat aagagaggaa ccgatagacc
      301 ttctggacag atcgttttcc tccagttcat ggccttttta gtgaataaat catcactaat
      361 tctataagtg tctggtcttg ttgataatat agttaatatg taacccttta gattcacgat
      421 ctgaagggtt gaaacatgac atacgttcaa agattaaccc ccgtttgaag gttagcttgt
      481 tagttacatt tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg
      541 ctttcttatc tattctttca atcagcactt cacccaattc atccttaatt ctaataactg
      601 gctattgcgc cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa
      661 caatatggca ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga
      721 aaatccggcc ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc
      781 agtcagttgt ctacttcttg ccttctgtgt ctggatgcta tttagcgcag ttaccgttaa
      841 tctcaataaa atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc
      901 ctccgtttct ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg
      961 acgccgatgg acggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat
     1021 tgccgtgcaa aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg
     1081 ttttgcaggt gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc
     1141 caaacaaggg agcgctcttg ggattaatgg cggattagga aacttaggtg taagtgtaat
     1201 gcagctggtt gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg
     1261 cgtaccgcag gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc
     1321 gctactggcg attgccacga tcgccgcatg gtcagggatg aatgatatcg ccagttcacg
     1381 cgcctcaatt gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct
     1441 gctttacctt gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc
     1501 aaaaacccag ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttatcgg
     1561 tgccatcgcg cggtcggttg gtggtgctat ttccgataag ttcggcggcg tgcgggtgac
     1621 gttgatcaac tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg
     1681 cacaggctcc ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgactgc
     1741 gggtctggga agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat
     1801 ttatcgggta aagatgaaag gcggtagtga tgagcaagct cagaaagaag ccgtcaccga
     1861 aacggcggcg gctctgtgct ttatctcagc cattggcgca gtgggcggct tttttattcc
     1921 gcaggcgttt ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt
     1981 tttaatcttc tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt
     2041 cagccaaaaa taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca
     2101 gatcccaatt cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg
     2161 ctacttcaaa caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa
     2221 ccgcgactgg gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac
     2281 ccacggtgtt aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt
     2341 gacctgggaa atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga
     2401 acctcgcggc tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct
     2461 gaaatacccg ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaagcaaca
     2521 cagcgatccg gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta
     2581 caaacaagtg cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct
     2641 gattgccgcc gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt
     2701 ctcgccgatc ccggcgatgt cgatggtttc ttacaccgcc ggaacgcgtt atctgtcgct
     2761 gcttggcggc acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc
     2821 gatgacctgg ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta
     2881 catcatcgcc tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac
     2941 cgaagtacgc tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc
     3001 caaattgtgc gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc
     3061 aatgggccat gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa
     3121 ctactgccgc cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg
     3181 tagctacgtt cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag
     3241 caacaatccg cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa
     3301 cggttcgatt ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc
     3361 cggtacggaa accgaattgt cgttaaccct gctcggtcaa catgacgctg ttgcaggcgt
     3421 ggccttcccc tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc
     3481 ggtgctggtg cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc
     3541 ggtggtcagc gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga
     3601 tgaaaacagt gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca
     3661 aattaccggc gtgccgcgcc agtatattga aaccatcgcc cgtgaatttg ccgataccgc
     3721 ccataaaacg catgggcgct cgatgattat cctcggcgca ggtgttaacc actggtatca
     3781 catggacatg aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca
     3841 aagcggtggc ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg
     3901 gttgccgctg gcctttgcgc tcgactggaa ccgaccacca cgccagatga acagtacctc
     3961 gtttttctac aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact
     4021 ttcaccgctc gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc
     4081 cgaacgtatg ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa
     4141 agctgaagcc gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc
     4201 gggcgattta cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt
     4261 gtttgtctgg cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa
     4321 gtatctgctg gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat
     4381 caaaccggaa gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt
     4441 gacgctcgac ttccgcatgt ccagtacctg cctgttctcc gatatcgttc tgcccaccgc
     4501 cacctggtac gaaaaagacg atatgaacac ctcggatatg catccgttta ttcatccgct
     4561 ttctgcggcg gtcgatccgg cgtgggaatc acgcagcgac tgggaaatct acaaaggtat
     4621 cgccaaagca ttttcgcaag tgtgcgtggg tcatcttggc aaagaaaccg acgtggtatt
     4681 acaaccccta ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg
     4741 gcgcaaaggc gaatgcgatc tgattccagg caaaaccgcg ccgaatattg tggcggtgga
     4801 gcgcgactac cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact
     4861 tggcaatggc ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa
     4921 actcaattac accaagcgtg atggcccggc acaggggcgt ccactgattg acaccgctat
     4981 tgatgcatca gaggtcattc tggcactggc tccagaaacc aacggtcatg ttgcagtcaa
     5041 agcgtggcag gcgctgggcg aaatcaccgg acgcgaacat acccatctgg cgctgcacaa
     5101 agaggacgag aagattcgct ttcgcgatat tcaggcgcag ccacgtaaaa ttatctccag
     5161 ccccacatgg tccggtctgg aaagcgatca tgtttcctat aacgcgggat acaccaacgt
     5221 tcatgagtta attccgtggc gcacgctgtc gggacgccag caactctatc aggatcatcc
     5281 gtggatgcga gcttttggtg aaagcctggt ggcttatcgc ccgcctattg acacccgtag
     5341 cgtcagtgaa atgcgccaaa tcccgccaaa cggcttcccg gaaaaagcac ttaacttcct
     5401 gacgccgcac cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac
     5461 gctttctcgc ggtggaccga ttgtctggat cagcgaaacc gatgcccgag aattgaccat
     5521 tgtcgataac gactgggtgg aagtgtttaa cgccaatggc gcgctgacgg cccgcgcggt
     5581 ggtcagccaa cgtgtaccgc cgggtatgac catgatgtat cacgcccagg aacgcattat
     5641 gaatatccct ggttcggaag taactggcat gcgcggcggc attcataact cggttacccg
     5701 cgtttgcccg aaaccaacgc atatgattgg cggttacgcg cagctggcct ggggctttaa
     5761 ctactacggc accgtcggct caaaccgtga tgaattcatc atgatccgca agatgaagaa
     5821 cgttaactgg ctggatgatg aaggtcgcga tcaggtacag gaggcgaaaa aatgaaaata
     5881 cgttcgcaag tcggcatggt gcttaacctc gataaatgta tcggctgcca tacctgttcg
     5941 gtgacctgta aaaacgtctg gaccggacgc gaaggcatgg agtacgcatg gtttaacaac
     6001 gtcgaaacca aaccgggcat tggttatccg aaaaactggg aagatcagga agagtggcaa
     6061 ggcggctggg tccgcgatgt gaatggcaag atacgcccgc gtctgggcag taagatgggc
     6121 gtaataacca aaatcttcgc caacccggtg gtgccgcaga ttgatgatta ctacgaacct
     6181 ttcaccttcg actacgaaca tttgcatagc gcaccggaag gcaaacatat ccctactgct
     6241 cgcccgcgtt cgctgattga cggtaagcgg atggacaaag tgatctgggg gccaaactgg
     6301 gaagaactgc tgggcggcga gtttgaaaaa cgtgcccgcg accgcaactt cgaggccatg
     6361 caaaaggaga tgtacgggca gtttgaaaac accttcatga tgtacctgcc gcgcctgtgc
     6421 gaacactgcc tcaatcccag ctgcgtggcg acctgcccaa gcggcgctat ctacaaacgc
     6481 gaagaagacg gcattgtgct gattgatcag gataaatgcc gtggctggcg tttgtgcatt
     6541 agcggttgtc cgtacaaaaa aatctacttc aactggaaaa gcggcaagtc agaaaaatgt
     6601 atcttctgtt acccacgaat tgagtccggt caaccgaccg tttgctcaga aacctgcgtg
     6661 ggtcgcatcc ggtatctggg cgtgctgctt tacgacgccg accgcattga ggaagcggca
     6721 agcaccgagc gcgaggttga cctctatgaa cgccagtgcg aagtgttcct cgatccgcac
     6781 gatccctcag tgatcgagga agccctgaaa caaggtattc cacaaaatgt gattgacgct
     6841 gcccagcgtt cgcctgtcta caaaatggcg atggactgga aactggcgct accgttgcac
     6901 cctgaatacc gcaccctgcc gatggtctgg tacgttcctc cgctgtcacc gattcagtct
     6961 tacgctgatg cgggcggttt gccgaaaagc gaaggcgtgc tccccgccat cgaaagcctg
     7021 cgtattccgg tgcaatatct cgccaatatg ttgagtgccg gtgatactgg tccggtactg
     7081 cgggcgctga aacggatgat ggcgatgcgc cactatatgc gttcacaaac cgtggaaggc
     7141 gttactgata ctcgtgccat cgacgaagta ggcctgagcg tcgcccaggt cgaagaaatg
     7201 tatcgctatc tcgccattgc caactatgaa gatcgttttg ttatcccgac cagccatcgg
     7261 gaaatggcgg gcgatgcctt cgcagaacgc aacggctgcg gttttacctt tggcgacggt
     7321 tgccacggtt cggacagtaa attcaacctg ttcaacagta gccgtatcga tgccatcaac
     7381 atcaccgaag tgcgcgacaa agcggagggc gaataatgca gatcctcaaa gtgatcggcc
     7441 tgttgatgga gtatccggac gagctgttgt gggaatgcaa ggaggacgcg ctggcgttga
     7501 tccgccgcga cgcgccgatg cttacggatt tcacccacaa cctgcttaac gcgccgctgc
     7561 tggataaaca ggccgaatgg tgcgaagtgt ttgaccgcgg gcgcaccacg tcgctgctgc
     7621 tgttcgaaca tgttcatgcc gagtcccgcg atcgcggcca ggcaatggtg gacctgctgg
     7681 cggagtatga aaaggtcggc ctgcagctgg attgtcggga actgcccgat tatctaccgc
     7741 tgtatctgga gtatttaagc atactgcctg acgatcaggc gaaagaagga ttgctcaatg
     7801 ttgcgccgat cctcgccctg cttggcggtc gcttaaaaca acgcaaggca ccgtggtacg
     7861 cgttgtttga tgctctgctg caactggcag gaagtacact ttcaagtgac agtgtcacaa
     7921 aacaagtaaa cagcgaagag cgcgatgaca cccgccaggc gcttgatgca gtgtgggaag
     7981 aggaacaggt gaagtttatt gaagataacg ccacggcgtg tgacagctcg ccgcttaatc
     8041 aatatcagcg acgctttagc caggatgtcg cgccgcagta tgtcgacatc agtgcgggag
     8101 gtgagaaatg attcagtatc tgaacgtctt tttttacgat atctacccgt acatttgtgc
     8161 gacggtgttt ttcctcggca gctggctgcg ctacgactac gggcagtaca cctggcgcgc
     8221 ctcctcaagt cagatgctcg ataaacgcgg gatggtgata tggtcgaatc tgttccatat
     8281 cggcattttg gggatcttct tcgggcacct gttcggcatg ttaacgccgc actggatgta
     8341 cgcgtggttt ttgccagtgg cagcgaaaca actaatggcg atggtgctcg gtggtatttg
     8401 cggtgtgttg acgttgattg gcggcgcagg actgctgtgg cgcaggctga caaatcagcg
     8461 cgtgcgtgcc acttccacta cgccggatat catcatcatg agcattttgc tgatccagtg
     8521 tctgttgggg ctaagtacca taccgttctc agcacagtat cctgatggta gcgaaatgat
     8581 gaagctggtg ggttgggcgc aaagcattgt cactttccgt ggtggatcgt cagagatgct
     8641 taacggtgta gcgttcgtct tccgcctgca tctggtgttg ggaatgacca tcttcttgct
     8701 cttcccgttc acccgactgg tgcatgtgtg gagcgccccg tttgagtact ttactcgtcg
     8761 gtatcagatt gtgcgctcgc ggcgataatt tcaccctggc cggatagccc ttcgctctcc
     8821 ggccacttgc aggctatgat agttctccag tcacaggaga acgttaatga aaccgcaggt
     8881 gtaccacgtc gatgctttta cctcacaacc gtttcgcggc aattctgccg gtgttgtttt
     8941 ccccgccgat aatcttagcg aagcgcaaat gcagcttatc gcccgcgagt taggccattc
     9001 ggaaactgct tttctgctgc acagcgacga cagcgatgtg cgcatccgct actttacgcc
     9061 aacggttgaa gtgccgattt gcggtcacgc gacagtagct gcgcactatg tacgtgcgaa
     9121 ggtgttaggt ttaggaaatt gcacgatctg gcaaacatcg ctggcaggaa aacatcgcgt
     9181 gactatcgaa aagcacaacg atgattatcg tatttcgctg gaacaaggta cgccgggctt
     9241 tgagccaccg ctggaaggtg aaacacgtga ggcgattatc aacgcgcttc atctcactga
     9301 agacgatatt ttgccgggct tgccaattca ggtcgcaaca acaggacact caaaagtgat
     9361 gatcccgttg aaaccggaag tggatatcga cgccctttcg cctgatcttg ctgcgctgag
     9421 cgctatcagc aaacagattg gctgcaatgg tttcttcccg ttccagatcc gcccaggcaa
     9481 aaacgaaacc gatggtcgta tgttctcgcc tgcgattggt attgtggaag atccggtgac
     9541 tggcaatgcc aatggcccga tgggtgcatg gttggtacat cacaacgtat tgccccacga
     9601 tggcaacgtg ttatgtgtta aaggccatca ggggcgcgca ttggggcgtg acggcatgat
     9661 tgaagtgacg gtgacaattc gtgataacca accggaaaaa gtcaccattt caggcacagc
     9721 ggtgattttg tttcatgccg aatgggcaat tgaactctga aaccgttgcc tgatgcgctt
     9781 cgcctgtcag gtctacaacc gagctaatgt atgccggata aggcgtttac gccgcatccg
     9841 gcaacctgac ataaattatt ttcccgcctc cgggtgcgta tcaaacgccg ccatcaccag
     9901 cgctaactca tccacggtaa agccatgttt cgcatcatcc acgcccagac caaactgttc
     9961 ttgcatcaca gcatataatg acgccacatc cggtagattt cgctgctcca ccgcgtgccc
    10021 attttcataa tgggtaaaat gaaaattggt cagcgttagc ttgccaccgt ccggcaaatg
    10081 gcggcacatc agcaaatgat ggcgaaaatg cgactgcggc cagtgcgccg accagaaatt
    10141 gcccatcaca taatcgcttt gttgctgctc gcagagatca aaacggtaca tcgactgcca
    10201 gtgctgatga tgattaaact gcaacaccca atcatcaccc tcctgcaaca accgataatc
    10261 tccgtgtggc gtggtctgca cgagatcgga aactaaacga atcggcgcgg ttagcgtctg
    10321 cccaccgaaa ccgacatcag caatccattt ttcctcttcc agttccacca acagcaaacg
    10381 atgggtgcgc ggcggtaatg ctggcggatt tgataacact acgcgcccta acaagctgcg
    10441 aacgttaaac tccagctcgc gtaacacccg ctcaaacacg ccattctgct caaaacagta
    10501 accgccacga cgggctatca ccagtttctc ttccagcgat tgatcatcaa gctgtatttc
    10561 cctcggcagc aaaacgtcaa ggttttcaaa cggaatggtg caattgtgtt tcaggtgcaa
    10621 tgcacgaagc gtatcaatat tgaccgcagc agctcccgac cagttaatac gggcaaaata
    10681 gtgattcaga atgggcgtca tcaggcgttt cccttagtgg ttttctcaac ttataacctg
    10741 gaatagacgc cgcaccggct attttgtgcg atcgcggaca tgcatcacgt ttgcaaatcg
    10801 ccaatcccgt taatttcgtt acttattgcg ttacggacta cgcttaaaaa aaccttcttc
    10861 gcgaggcgat accatgagcc gattcatccc catagaatta caccatgcca gccgtctatt
    10921 aaatcatggc ccaactgtca tgatcaccag tttcgatgag caatcccagc ggcgtaacat
    10981 tatggctgca gcctggtcaa tgccggtgga gtttgaaccg ccacgcgtgg cgattgtggt
    11041 agataaatcg acatggacca gggagttgat tgaacgtaac ggtaagtttg gcatcgttat
    11101 cccgggcgtt gcagcaacta actggacgtg ggcagtggga agcgtgtcgg gtcgtgaaga
    11161 agataaattt aattgctatg gcattccggt ggtgaaaggt ccggtgcttg gtttgccact
    11221 tgttgaagag aaatgcctgg cgtggatgga gtgccgcctg ctacctgcga cttctgcgca
    11281 acaaaaatac gacacgctgt ttggcgaggt ggtatcagca gcggcagacg cacgggtatt
    11341 tgtcgaaggc cgctggcagt ttgatgatga taagctcaat acgttgcatc atttaggtgc
    11401 tggcacgttt gttactagcg gcaagcgtgt tacggcaggt taagcattac atgctgtaac
    11461 cgggtttctt tatcaaagcc tccatttggg gcgcgatttc ggcatcccag atagcttgcc
    11521 aagattctgg ttgaatctgc tgtagagcaa tgcttatcga actgtcttta ctgttcagat
    11581 ggcgaataat aacgtcggta atatctgcag caagtgctgc tttttgttgt tcgtccagtt
    11641 cacgcggaaa gcatttaatg tcgatgtgcg gcatgattac gctccttttg aaagatgtca
    11701 gtaaaattaa cacacattgt gaatgtcatg tgtccggtaa agcgtttata cgacaataat
    11761 gccagggcaa gattacgaaa gccttctccc cgcaaggact gacgccaggt agtttctgtc
    11821 catggctgct tttcgcatct tacgtcttaa ccctgccttg aataccttat cattcgtcaa
    11881 aatattaata gcaatgtgcc gtatccctga aaataattct gctgcatttc cttttcttat
    11941 tttgcagtcg tcttcattca ttaccacgtc cagacgccag tgcagcttat tctccacgtg
    12001 ccagtggttt cggattgctg tggcgaactt ctctgcggtt aaatcagcag aactgatata
    12061 atatctgacc gtcatttctg gctctttctt ttgttctgct attatggacc gaaaggagac
    12121 tgccacgcat aatttcttca gccctttcca ttcaaacgtg aaatcaataa gttcatcagg
    12181 gacatcgcaa acaatatgaa gacggatttc ttctctgccg tgactctttt cactcattgc
    12241 gtaactgtca tgctctggat tatttaattc tttcagcgga aatttttcct caaaggcttt
    12301 atttagccgc ccctggtttc cttttaccgc gaataaataa tcacctccct gtttttgtat
    12361 cttttctgca atatctttct ggcagcccat cgcatcagtt gtgatgattt ttcctttaat
    12421 atccatcatg ttaagaagtt caggaatggc tgtaatctca ttggatttct catccgtctt
    12481 gatctgtccg aggaccagac tatgcattgt tgagaacgca ctaatgacat gaatcgctcc
    12541 cctgcgacga ctcttgtcat aagagtgccg gagcgttttt ccatcaattg caatgacgtc
    12601 tttatcatct gaagaatggc agtcacgcat ccagttaata aagcactcgt gaaattttgc
    12661 aggactgata caggatacaa ctctggcaat gatatcgtga acaggaatac cattttcaaa
    12721 atcaccatat cacttcaaaa catcgggatg tgtttcccca aaatcctcta tatcttccca
    12781 gccttctgca ccagaaataa cggcacaaat agtcaacagt agaatatccg ataatttatg
    12841 ttccactttc caggcttgtc tgtaatcggg gataatagaa atatgttcca tcattttttt
    12901 aagttccatt ttgttctcct taattatgta aggagtattt gatcatgtat aagcaataaa
    12961 aaacagcttc aggtaatgag gaatatctca atttttaaac ataaaatgcg aattatttag
    13021 tacaaaaagt gaacttttga tgatctttcc ctaatctcac ataggatcag atctcgtctg
    13081 atcgttcccc ctgcgctcac taccatggct ctttaccaca gcagccgggg ttgtttggga
    13141 ccgacttccg acagcagatc ccggtgggcc tgccaccatc tcttcacaag cttatgctta
    13201 ttacatgcaa aacgtcccgt tttgcatctt tgtgcctacg gcacaccgat gatctttccc
    13261 tgtgatcttt ccctgaatct gcccctgaga acctgaatca aacgtctgac tcgcagaacc
    13321 atctgtttct cgcgctctgt agaacttcat gaaaaagtca tcggtgcttt tatcgaaaaa
    13381 tacatgttct actaattgaa gtcatcatca a
//
LOCUS       DAZGAN020000129        13273 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGAN020000129 DAZGAN020000000
VERSION     DAZGAN020000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.129"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            129..695
                     /locus_tag="ACHKYO_RS20720"
                     /old_locus_tag="pgaptmp_004144"
     CDS             129..695
                     /locus_tag="ACHKYO_RS20720"
                     /old_locus_tag="pgaptmp_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4430901.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            746..3205
                     /locus_tag="ACHKYO_RS20725"
                     /old_locus_tag="pgaptmp_004145"
     CDS             746..3205
                     /locus_tag="ACHKYO_RS20725"
                     /old_locus_tag="pgaptmp_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4430902.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3220..3951
                     /locus_tag="ACHKYO_RS20730"
                     /old_locus_tag="pgaptmp_004146"
     CDS             3220..3951
                     /locus_tag="ACHKYO_RS20730"
                     /old_locus_tag="pgaptmp_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4430903.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3948..5009
                     /locus_tag="ACHKYO_RS20735"
                     /old_locus_tag="pgaptmp_004147"
     CDS             3948..5009
                     /locus_tag="ACHKYO_RS20735"
                     /old_locus_tag="pgaptmp_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4430904.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKILVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5161..6207
                     /locus_tag="ACHKYO_RS20740"
                     /old_locus_tag="pgaptmp_004148"
     CDS             5161..6207
                     /locus_tag="ACHKYO_RS20740"
                     /old_locus_tag="pgaptmp_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGU4430905.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRFPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6204..6995)
                     /gene="nei"
                     /locus_tag="ACHKYO_RS20745"
                     /old_locus_tag="pgaptmp_004149"
     CDS             complement(6204..6995)
                     /gene="nei"
                     /locus_tag="ACHKYO_RS20745"
                     /old_locus_tag="pgaptmp_004149"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HGU4430906.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7031..7765)
                     /gene="pxpA"
                     /locus_tag="ACHKYO_RS20750"
                     /old_locus_tag="pgaptmp_004150"
     CDS             complement(7031..7765)
                     /gene="pxpA"
                     /locus_tag="ACHKYO_RS20750"
                     /old_locus_tag="pgaptmp_004150"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HGU4430907.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7755..8687)
                     /gene="pxpC"
                     /locus_tag="ACHKYO_RS20755"
                     /old_locus_tag="pgaptmp_004151"
     CDS             complement(7755..8687)
                     /gene="pxpC"
                     /locus_tag="ACHKYO_RS20755"
                     /old_locus_tag="pgaptmp_004151"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HGU4430908.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8681..9337)
                     /gene="pxpB"
                     /locus_tag="ACHKYO_RS20760"
                     /old_locus_tag="pgaptmp_004152"
     CDS             complement(8681..9337)
                     /gene="pxpB"
                     /locus_tag="ACHKYO_RS20760"
                     /old_locus_tag="pgaptmp_004152"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HGU4430909.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9360..10103)
                     /gene="ybgI"
                     /locus_tag="ACHKYO_RS20765"
                     /old_locus_tag="pgaptmp_004153"
     CDS             complement(9360..10103)
                     /gene="ybgI"
                     /locus_tag="ACHKYO_RS20765"
                     /old_locus_tag="pgaptmp_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HGU4430910.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVLRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10374..11855
                     /gene="dtpD"
                     /locus_tag="ACHKYO_RS20770"
                     /old_locus_tag="pgaptmp_004154"
     CDS             10374..11855
                     /gene="dtpD"
                     /locus_tag="ACHKYO_RS20770"
                     /old_locus_tag="pgaptmp_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HGU4430911.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSSMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11897..>13273)
                     /gene="phrB"
                     /locus_tag="ACHKYO_RS20775"
                     /old_locus_tag="pgaptmp_004155"
     CDS             complement(11897..>13273)
                     /gene="phrB"
                     /locus_tag="ACHKYO_RS20775"
                     /old_locus_tag="pgaptmp_004155"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HGU4430912.1"
                     /translation="HDNLALAAACRNSSARVLALYIATPRQWATHNMSPRQAELINAQ
                     LNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFYNYQYEVNERARDVE
                     VERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLKRLREGMPECVAAPK
                     VRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQNGAGEYEQQRDFPAV
                     EGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSELIWREFYRHLMTYYP
                     SLCKHCPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQLNSTGWMHNRLRMIT
                     ASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPITQG
                     EKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQPIVDHKEARLRTLAA
                     YEEARKGA"
BASE COUNT         3187 a         3586 c         3211 g         3289 t
ORIGIN      
        1 catcaattaa ttacttttac atttaataaa attaataata tttttaagaa aaactaaaaa
       61 cattcgacaa tcaaataaga tattcttgca ccgatatact tttttctttt ctgttaaagg
      121 actccgttat gtttaaagga caaaaaacat tggccgcact ggccgtatct ctgctgttca
      181 cagcacctgt ttatgcggct gatgaaggat ccggtgaaat tcactttaaa ggtgaagtta
      241 ttgaagcacc gtgtgaaata catcaggatg atattgataa agaggttgaa ctcggtcagg
      301 tgaccaccag ccacattaat cagtcacatc acagcgatgc cgttgctgtc gacctgcgct
      361 tagtcaactg tgatctggaa aactccagca acggttccgg tggcaagatt tctaaagttg
      421 cagtcacctt tgatagctca gcgaaaacca ccggcgcaga tccgattctc aacaacacca
      481 gcacgggtga agccaccggc gtcggcgtac gtttaatgaa taaagatcaa agcaatatcg
      541 ttttaggcac cgctactcca gatatcgacc tggctccgac ctccagcgaa caaacgctga
      601 atttctttgc ctggatggaa caaattgatc aggctacacc tgtaacgcca ggcgcagtta
      661 cggcgaatgc aacctacgtg ctggattata aataagtatt actttggcgg cgctatcgcc
      721 gcctttcata aaatatttat caggtatgga caccgtgaat atttatcgac tctcttttgt
      781 atcctgcctg gtcgtggcga tgccttgcgc attggcggtc gaattcaacc ttaatgttct
      841 cgataaatca atgcgcgacc gcattgatat ttcattatta aaggaaaaag gagtcattgc
      901 tcccggtgag tattttgtta gcgttgcggt aaataataac caaatcagta acgggcaaaa
      961 gattaactgg cacaaaaatg acgataaaac cattccgtgc atcaatgatt tactggtcga
     1021 taaatttggc ttaaaacctg aagtccgtca gtcgttacca ttgataaatc agtgcgtcga
     1081 ttttagctcc cgacctgaaa tgctcttcaa tttcgatcaa gccaatcagc aactaaatat
     1141 caccattccg caagcctggc tggcgtggca ctcagaaaac tggaccccac cctccacatg
     1201 gaaagaaggt gtcgccggtg tcctgatgga ttacaacttg tttgccagca gctaccgccc
     1261 acaggatggc agcagcagca ctaatcttaa cgcctatgga acagccggaa tcaacacggg
     1321 ggcatggcgc ttacgcagtg attatcagtt gaatcagact gatagcgatg ataaccatga
     1381 acagtcaggc gaaatatcac gtacctatct ttttcgcccg ttaccgcaat taggttcaaa
     1441 attaaccctc ggcgaaacgg attttagttc caatattttc gacggttttt cttataccgg
     1501 cgcggcactg gcaagtgacg atcgaatgtt gccatgggaa ctacgcggct acgccccaca
     1561 aattagcggt attgcacaga ccaatgccac ggtgacgatc agtcaatcag gccgcgtcat
     1621 ttaccagaaa aaagtcccac caggcccatt tatcattgac gaccttaatc agtctgttca
     1681 gggcacactg gatgtcaaag tgacggaaga agatggtcgg gtgaacaatt tccaggtttc
     1741 ggcagcatcg acgcccttcc tgactcgtca gggacaggtt cgctataaac tggccgcggg
     1801 tcagccacgg ccctccatgt cacatcaaac tgaaaatgaa acctttttta gcaatgaagt
     1861 ttcctggggg atgctgtcaa acacctcgct gtacggcggc ctgctgcttt ctggtgatga
     1921 ctaccattct gccgcaatag gtatcgggca aaatatgctg tggcttggcg cgctgtcgtt
     1981 tgatgtcacg tgggccagta gtcactttga tactcagcag gacgagcggg gcttaagcta
     2041 ccgttttaat tacagcaaac aagtggatgc cactaacagc acgatttccc tcgccgctta
     2101 tcgtttctcc gatcgtcatt ttcacagcta cgccaactat ctggatcaca aatacaacga
     2161 cagcgatgcg caggacgaaa aacagacgat cagcttatct gtgggccaac cgattacccc
     2221 actaaacctc aatctttacg ccaacctgct acatcaaacc tggtggaatg cagacgcctc
     2281 cacgaccgcc aacatcacgg caggttttaa tgttgatatt ggtgactgga gagatatctc
     2341 tatttctacg tcattcaata caacccatta cgaagataaa gatcgcgaca accagattta
     2401 cctgtcgatt tcgctcccct tcggtaacgg tggtcgggtt ggctatgaca tgcaaaacag
     2461 tagccacagc accacacacc gcatgtcgtg gaacgatacg ctggatgaac gtaatagctg
     2521 gggcatgtct gccggactgc aatccgaccg tcctgacaat ggagcccagg tgagcggtaa
     2581 ctatcagcac ctgagttcag cgggtgagtg ggatatttct ggtacctatg ccgccaatga
     2641 ttacagttcc gtcagcagca gctggagcgg ttctttcacc gcaacccaat atggtgcagc
     2701 gtttcatcgc cgcagctcca ccaatgaacc acgcctgatg gtcagcaccg atggcgtggc
     2761 agatattccg gttcagggca atctcgacta caccaaccat tttggcattg cggtggtgcc
     2821 gttgatttcc agttaccagc cttccaccgt ggcggtgaac atgaatgact tacccgacgg
     2881 cgtaacagtt gcagaaaacg ttatcaaaga aacgtggatt gaaggcgcga taggttacaa
     2941 atcactggct tcccgttccg gtaaagacgt taacgtcatc attcgcaacg ccagcggtca
     3001 gtttcctccc ctcggagcgg atatccgcca ggatgacagc ggcattagcg tggggatggt
     3061 tggcgaggaa ggacatgcct ggttaagcgg agtcgctgaa aatcaaaagt ttaccgtggt
     3121 ctggggtgat agccagcatt gctcgctcca tcttcctgaa catatggaag acaccgcaaa
     3181 tcgcctgatt ttaccttgtc attaattaag gaaatgaaaa tgacatttat gaaaggacta
     3241 ccccttttgt tgttggttgc cagtctgtgc agccacgctg cactacaacc cgatcgcact
     3301 cgtattgtgt ttaacgctaa tgataaagct accagtctgc gggtggataa tcgtagcgat
     3361 aaactcccct acctcgctta ttcctggctc gaaaatgaaa aaggggagaa aagcgatgat
     3421 cttctggtcg ccctaccgcc gatacaacgt ctggaaccga aagcaacaac gcaggtacgg
     3481 atagtaaagc aagcctcgac cacaaaatta ccgggcgatc gcgaaacgct atttttctac
     3541 aatatgcgtg aaattcctcc cgccccggaa aaaaacagcg accatgccgt tctgcaagtc
     3601 gccattcaga gtcgtatcaa ggtgttctgg cgaccggctg cattacgcaa gaaagcagga
     3661 gaaaaggtcg aactgcagtt acaggtcagc cagcagggca accaactgac actgaaaaat
     3721 cctaccgcgt attatctgac cattgcctac ctgggacgaa atgaaaaagg cgttctcccc
     3781 ggcttcaaaa ccgtgatggt tgcgcctttt agtacggtca acacaaacac cggaaattat
     3841 agcggcagcc agttttatct cggttatatg gatgattacg gcgcgttacg catgacgacg
     3901 ctcaactgca gcggacaatg ccatttacaa gccgtggagg cgaagaaatg agtgctggca
     3961 agggagtgtt gctcgtcatc tgtctgttat ttctgccgtt gaagtctgcg cttgccctga
     4021 actgctactt tggttcatcc ggtggctcgg tagaaaaatc agaagcgatt caaccttttg
     4081 ctgtaccagg caatgccaaa ctcggcgata agatttggga atctgacgat attaaaattc
     4141 tcgtctattg tgacaacaat accaacggca attttgaaag tgagcatgtt tatgcctggg
     4201 taaaccccta cccgggggtg caggatcgct attatcaact cggcgtaact tacaatggcg
     4261 ttgattatga cgccaaccag ggaaaaagcc gcatcgacac taaccagtgt attgacagta
     4321 agaacattga catttatacc cctgagcaga ttatcgcgat gggatggcag aacaaaattt
     4381 gctcgggtga tcccgctaat attcacatgt cgcgtacctt tctcgctcgc atgcggttat
     4441 acgtcaaaat acgagaaatg ccgccgcatg attatcaaag tacgcttagc gactatatcg
     4501 tcgtgcaatt tgacggtgcc ggtagcgtta acgaagaccc tactgcccaa aacctgaaat
     4561 atcatattac tggtctggaa aacattcgcg tgctggattg cagcgtcaat ttttccattt
     4621 caccggaaac acaggtgatt gattttggta aatttaattt gctggatatt cgtcgccaca
     4681 caatgtcgaa aacattcagt attaagacga caaaatcaca aaatgaccaa tgcaccgacg
     4741 gatttaaagt cagttcctcc ttttataccg aagaaacgtt ggttgaagaa gataaagcat
     4801 tgttgattgg taatggtctg aagctacgtt tgctggatga aaacgcctcg ccttatacct
     4861 tcaataaata tgctgaatac gccgatttca ccagcgacat gttggtctac gaaaaaacct
     4921 atacagctga actttcgtcc attgcaggta cccccatcga ggctgggccc ttcgatacag
     4981 tggtgctttt taaaattaac tataactgat ttgccatgat gccctttgca gccggaaagg
     5041 gcatctcttt cagaaaattg ctgattttcc ccttttcctt gattgctgtt attttttcct
     5101 ggcccggctt tctttatgct gtatcgatac accaaagaga aaataatgag ggagcgaagg
     5161 atgccagttt tgcagtgggg aatgttatgt gtgctatcac ttctcctttc tattggtttc
     5221 ctcgcggtac atctcccggc ggcgctatta cttgggccga tgatcgccgg gatcatcttc
     5281 agtatgcgcg gaattactct gcaacttccc cgctccgctt ttctcgccgc gcaggccatt
     5341 cttggctgca tgattgcgca aaacctcact ggttcgattc tcaccacgct ggccgccaac
     5401 tggccgatcg tgcttgctat tttactggtg acgctgcttt ccagcgccat cgtgggttgg
     5461 ttattggtgc gctatagctc attgcctgga aataccggtg cctggggttc ttctcctggc
     5521 ggcgcggcgg caatggtcgc tatggcgcaa gattacggcg cagatattcg cctggttgca
     5581 tttatgcaat atctgcgagt gctgttcgtc gcgggggccg ctgttttggt cacccgcatg
     5641 atgctgggtg ataacgctga agcggttaac cagcaaatcg tctggttccc gccagtgagc
     5701 attaatctcc tgcttacgat tttgctcgcg gtcgttgccg gtacggcggg atgcttgcta
     5761 cgttttcctt ccggcacgat gctcatcccg atgctggcgg gcgcagtgct ccagtctggt
     5821 cagctcatca ccatcgaact cccggaatgg ctgcttgcga tggcgtatat ggcaattggc
     5881 tggcggattg gtcttggttt cgataaacaa atcttactgc gggcactacg cccgctaccg
     5941 caaatcctgc tgtcgatttt tgctctgctg gctatttgtg cgggtatggc gtgggggctg
     6001 acccggttta tgcacattga ttttatgact gcctacctcg ccaccagtcc cggcgggctt
     6061 gataccgtgg cggtcatcgc cgcagggagc aatgccgata tggcgcttat tatggcgatg
     6121 caaaccctgc gcctgttcag tattttgctg acggggccag ccattgcgcg gtttatttca
     6181 acctatgcgc cgaagcggtc ggcctagtgc tggcagcctg gacaccagta aaacgggcgc
     6241 gaggacaacg tggttttctc aatgatgccg ccacagcgtt cgcacagttc gccatcacga
     6301 tgaaaaacct taaagcgaaa cagcgcccca tgatgcttat tctcatccac ctgcccccgc
     6361 gtagcgtaag aaaaccgagg aatatccagt aacgcgtgtg cgagtgcatc cagttgcgcc
     6421 gcattgaggt ctttcgcttt atgatttcca gtcaacccaa cctgccagag gatctccacc
     6481 cgcaaataat tgccaagtcc tgccagaaac gcctgatcga gcagtaatcc agcaaactga
     6541 cggttacgaa agcgcggtga caataaccgt tcctttacca cctccggcgt cagattcgga
     6601 tccagcacat cgggaccaac gcgttgtaaa aacggatgcg tggtcagttg ttccggcctc
     6661 aacatctcaa tatccgaggc gctataaagc agaatggttt tgtcagccgt ttgcagtttt
     6721 acccgcaata ctcgcgtggt ctgcggctct tcgccggtat caaccacgcg ccagacgccg
     6781 taaagctgat tatggctgta gagcgttaag tcgttggaaa aatgagttaa caacgcctta
     6841 ccacgcgttt ccacatgggt aacgtgttga ccgataagtt gtgattgata agtttttaac
     6901 tgcgggaagg caaaccagac atcagttagt ggtttgcctt tgatcgccgc ctccaggtta
     6961 tccgctgcac ggcggatctc cgggccttca ggcatggtga tatccttttt tcattcttta
     7021 ttaatcgggg ttatgctgcg acaacaatct ccttttcggc aaatgtagag cgtagtcggc
     7081 gggcgaacgc cagtgcgtgc tcgccgtcgc catgcaggca gactgtttgc gccgtgaccg
     7141 ttgcccattc gccggtgata cttttgactc tgccgtgttg caccatctct agcgtttgcg
     7201 ccagcgcctg ttcttcgttt tcaatcaacg cgcctggctg acttcgcggc accagcgagc
     7261 cgtcagcctg ataaccgcga tcggcaaaca cttcctcgcg cgttgtcagg ccatattgct
     7321 tgcctgcacg aatcagctcg cttcctgcca gcccaacgag aaccaatgct ggatcgcaag
     7381 cgtataccgc tctggcgatg gcgtctgcca gttgtgcttc tttcgccgcc tggttgtaca
     7441 acatgccgtg cggtttgaca tgacgcatta cgccgccttg cgcacgggca atcgttgcca
     7501 gcgcgccaat ttgatacagc gtctgggcgt aaacggtttc tggcggcagc tgcatggcgc
     7561 tgcgaccaaa attttccctg tcgggaaaac tcgggtgagc gccaatcgcg acaccatttt
     7621 ttattgcttc acgcacgcaa gcctgcatgg tttgggcatc gcctgcatga aatccacagg
     7681 caatattggc agaggaaacc agcgttaata gctctgcgtc gctggcgcag ccttcgccca
     7741 gatcggcgtt caggtcaatt ttcattgtgt agccgccacg ctaattgttc gaaataacgt
     7801 tgttgatctt gccgcgcttt cagtgcctct tccagtgaac actggacaaa atgaatcggc
     7861 tgaccgagcg gaatttgcgc cagatggtac atatcagcct caatgataca ggcaatacgc
     7921 gggtaaccac cggtggtctg tgcgtcgttc atcaacacaa taggctgccc gttatgcggc
     7981 acctgcacca cacccggtaa caaaccgtga gataacagtt cgcgatcggt ggtgcgtttt
     8041 aaaatttgcc cctgtaagcg atagcccatg cggttacttt gcgggctaag ctgccagggc
     8101 gaacgccaga atgcatcctg cgaggcgcga tcgaactcat gatattccgg ccccggcaag
     8161 gcgcgaatgc ggttgcccca cagcagctgt ttaacgccct gcgcttccat aaaatcacgc
     8221 ttcgatttgc caatcgggag tcggtcacca tccttcagta aacggccttc cagcccgcca
     8281 atccccacgt tgagatcggt gctgcatgac cccattaccg gcggaacatc aataccaccc
     8341 gcgaccgcca gataactgcg catcccgtgc tgcgggcgtt taagcgttaa acgctggcct
     8401 gctttcatcg gcaatcgcca gccggtccag acggcattat catccagccg cgcttcgcaa
     8461 ccggcccctg tcagagcaaa ccacccatca gtttcgaact caaccgttaa ctgaccgagc
     8521 gtgatctcca gcgcgggggc attggcgtca ttacccacca gcaggttagc aatgcgcagc
     8581 gcaggcatat ccagtgcgcc gcagtggctg atacccgact ggcgaaaacc gtgacgaccg
     8641 ccatcctgca cagtggtata catgcccgca cgaataatct tcagcatact ccctccttct
     8701 gcggtacaaa gcgcacgctg tctcccggac gtaataagat gggttcgtca cgcgccggat
     8761 caaacaggct gagtgaggta tgaccaatca actgccagcc acccggcgtt gccagcggat
     8821 aaacaccagt ctgcggcccg ccgatcccga cagaacctgc cggaacgagc aagcgcggtt
     8881 cagcgcgccg tggcgtgtgt agttgttccg gcaaactccc gagatacggg aagcccggtt
     8941 gaaaacctaa aaaccagacc acgtattcca cggaggagtg caattcaaca acctgttttt
     9001 cgctcaaccc gcaatgcgcc gcgaccaccg ccaaatccgg tccgcctgca ccaccgtaaa
     9061 ccaccggaat ttcaataaag cgagactcag gttccagggc ctcgctctcc tcccaccagc
     9121 gttgcaaacg ctctatggca tccagcgcca gcgactcagg attacgcaaa atcaccgtga
     9181 tattgttcat gccgggaatg gcttcaacca cattcggcat atccaccaga cgctgcgcca
     9241 gtcgccagat ccgtttctgg ctagccagcg tcaccggcgg ttccagttcc agcactaccg
     9301 ccgtttcacc tatcagataa caacgcgctc gttgcacttt cgatccctct gattattcgt
     9361 tatgcaggat taggaatatc aataaaggtc acatcaagat cggtattttc attcagccac
     9421 tcgctcaatg cgcgaatacc accacgttca gtggcatggt gacctgcagc ataaaaatgc
     9481 aatccctgct cgcgggctga atgaatggtc tgttcagaaa cttcgccagt aataaaagca
     9541 tccacgccaa aacgcgcggc gctatcgata aaactttgcc cgccgcccgt gcaccaggcg
     9601 acgcgcagga ccacctcagg tccggtatcg ccacaccata acggcttacg tcccagacgc
     9661 gcttcaatcc aggaggctag ttccagtccc ggcacaggca tggtcagctc tccccacggc
     9721 accaacggct caatttcgcc catgaccgtg atccccagta acgccgccag ctgtgcgtta
     9781 ttgcccagct caggatgtgc gtcaagcggc aaatgccagc catacaggtt gatatcattc
     9841 gccagcaacg tttttaaacg gttacgcttc attccgcgaa tgaccggaga ctctcctttc
     9901 cagaagtagc catgatgcac aatgaccgca tccgcgccca gacgcactgc ctcatcgagc
     9961 aaagcctggc tggcggtgac accggtaaca attttttgca ccgtctcttt gccttccacc
    10021 tgcaaaccat tcggcgcgta atcactaatc gccgcgctgt tcagtttttc gttaatcagt
    10081 tgttccagtt cggtgttttt catcttcgcg ccttccgtat tctgatagct acaccgttaa
    10141 catagcgtta cctcgccagt tcgtcgatag ccaatctttt gacatattaa gaaacaaacc
    10201 cgacgccaaa agacgcaaat tgtttatatc atcacgcttc agctaatgta tatttatgca
    10261 atcagacagc atattgcata aataatcaga tctgtatcaa agttttaact ttaaggaaac
    10321 acaagcacgc cgcgcaacta ttacgtgacg ttgccgtcaa ttgtggaatt attatgaata
    10381 aacacgcatc acagccgcgc gctatttact atgtcgttgc gctgcaaatc tgggaatatt
    10441 ttagctttta cggcatgcgt gccctgctga ttctctatct caccaatcaa ctaaaataca
    10501 acgatactca cgcctacgag ttatttagcg cctactgttc gctggtgtat gtcacgccaa
    10561 tcctcggtgg ctttttggcg gataaagttc tcggcaatcg catggcggtg atgctggggg
    10621 cgttattgat ggcgatcggt catgtggtgt tgggtgccag tgagatccat ccgtcattcc
    10681 tctatctgtc cctggcgata atcgtttgtg gctatggtct gtttaaatcc aatgtcagtt
    10741 gcctgctcgg cgagctgtat gagccaaccg atccgcgtcg tgatggcggt ttctcgctga
    10801 tgtatgcagc gggtaacgtg gggtctatta tcgcacctat tgcctgtggc tacgcccagg
    10861 aagagtacag ctgggcgatg ggctttggcc tggcggcggt gggtatgatc gcgggtctgg
    10921 tcattttctt atgtggcaat cgtcatttca cacatacccg cggcgttaat aaaaaagtac
    10981 tgcgtgcgac aaactttctc ctgcctaact ggggatggct gctggttctg ctggtggcaa
    11041 cgcctgcgct gattaccgta ttgttctgga aagagtggtc ggtatacgcc ttaattgtcg
    11101 cgactattat tggcctgggt gtactggcaa aaatttatcg caaagcagaa aaccagaaac
    11161 agcgtaagga gctggggctg attgtgacgc tcaccttctt cagtatgttg ttctgggcct
    11221 tcgcacaaca gggcggcagc tcgattagcc tttatatcga ccgcttcgtt aaccgcgata
    11281 tgtttggtta taccgttccg actgctatgt tccagtcgat taatgccttc gcagttatgc
    11341 tgtgcggtgt gttcctggcg tgggtggtta aagaaagcgt cgcaggtaat cgtaccgtgc
    11401 gcatctgggg aaaatttgct cttggtctcg gcctgatgag cgccgggttc tgcattctga
    11461 ccttaagcgc ccgctggtcc tcaatgtatg gtcactcttc tctgccactg atggtattag
    11521 gcctggcggt gatgggcttt gcggaactgt ttatcgaccc ggttgccatg tcgcaaatta
    11581 cgcgcattga aatccccggt gtgaccggcg tattaaccgg catttatatg ctgctttccg
    11641 gcgcgattgc gaactatctc gcgggcgtga ttgccgatca gacatcgcag gcttcgtttg
    11701 atgcttccgg ggcgatcaac tactccatca atgcatatat tgaagtattt gatcaaatta
    11761 cctggggcgc actggcgtgt gtaggactgg tactgatgat ttggctgtat caggcgctga
    11821 aattcagaaa ccgcgcgctg gcgctggaat cttaataggc cagcaccata cctgataaaa
    11881 cgctcgctgc gttttatcag gctcctttcc tcgcttcttc gtacgctgcc agcgttcgca
    11941 gccttgcctc tttgtgatca actatcggtt gcggataatc cagcatcaca cctgctttct
    12001 gcgcccactt ccacggctca tgcaccgctt tccctggtac atcgcgcagc tccggtaacc
    12061 atcggcggat aaactcgccc tcacggtcaa atttctcgcc ctgagttatc ggattgaaaa
    12121 tacgaaaata cggcgcagca tcagtacccg tagaagccgc ccactgccag ccaccgttat
    12181 tagctgccaa atcaccatca atcagctgcg acatgaaata tcgctcgcct tcgcgccagt
    12241 cgatcaacaa atctttcacc agaaaactgg ctgtaatcat ccgtagccgg ttatgcatcc
    12301 agccagtact gttaagctga cgcatagcgg catcaacaat cgggtatccc gttttgcctt
    12361 tctgccaggc ctgtaaatgt gcgggattgc tttgccactg cacacgatcc gtccaggcaa
    12421 taaacggaca atgtttacac aacgaggggt aatacgtcat cagatgacgg tagaactcac
    12481 gccagatcag ctcactaagc cagacactac cggccccacc gtccagcgcc tgcggctgtt
    12541 ccgccaacaa gcgatgcaaa cactggcgag gcgataaccc gcccgttgcc aggctggcgg
    12601 acaaacgact ggtgccttcc actgccggaa aatctcgttg ttgctcatat tctccggcac
    12661 cgttctggca aaactggcgt aattgcgcaa tcgccgcttt ttcttccacc gggaaatgcg
    12721 cagtatcgaa agactgacga ggataattca gcgtaatgga tggcgcgggc tctatcgatc
    12781 cgctactacg aacttttggc gcagcgacac actccggcat cccttcccgc agccgtttca
    12841 gccaggcatt cttaaaaggc gtaaagactt tgtacatctc gtgattaccg gtcatcaccg
    12901 cgccaggcgg caggatcacg ctgtcatcaa atccttcaca caccacgtta cgcagcgctc
    12961 tttcaacttc cacatcccgc gcccgctcat tcacttcata ctgatagtta taaaacaggt
    13021 gggtaacgct gttttccgcg cacacctgtt taactatttc gacactggcg acaaagtcat
    13081 ccacttcacg gaacaataaa ggaatacctt tttccgcaag cgctatttgt agcccattca
    13141 gttgagcatt gataagttca gcctgacgcg gcgacatgtt atgcgtcgcc cactggcgtg
    13201 gtgtagcgat atataacgcc agcacgcgtg cagacgaatt gcggcaggcg gcagccagtg
    13261 cgagattatc gtg
//
LOCUS       DAZGAN020000130        13254 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGAN020000130 DAZGAN020000000
VERSION     DAZGAN020000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.130"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..594)
                     /locus_tag="ACHKYO_RS20780"
                     /old_locus_tag="pgaptmp_004156"
                     /pseudo
     CDS             complement(1..594)
                     /locus_tag="ACHKYO_RS20780"
                     /old_locus_tag="pgaptmp_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
     gene            656..1807
                     /locus_tag="ACHKYO_RS20785"
                     /old_locus_tag="pgaptmp_004157"
     CDS             656..1807
                     /locus_tag="ACHKYO_RS20785"
                     /old_locus_tag="pgaptmp_004157"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HGU4430913.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            complement(1817..2077)
                     /locus_tag="ACHKYO_RS20790"
                     /old_locus_tag="pgaptmp_004158"
                     /pseudo
     CDS             complement(1817..2077)
                     /locus_tag="ACHKYO_RS20790"
                     /old_locus_tag="pgaptmp_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2453..2731)
                     /locus_tag="ACHKYO_RS20795"
                     /old_locus_tag="pgaptmp_004159"
                     /pseudo
     CDS             complement(2453..2731)
                     /locus_tag="ACHKYO_RS20795"
                     /old_locus_tag="pgaptmp_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2858..2944
                     /locus_tag="ACHKYO_RS20800"
                     /old_locus_tag="pgaptmp_004160"
     CDS             2858..2944
                     /locus_tag="ACHKYO_RS20800"
                     /old_locus_tag="pgaptmp_004160"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4430914.1"
                     /translation="MVKLALQPRGSVARIAQEHDINDNLLFK"
     gene            complement(3296..4552)
                     /locus_tag="ACHKYO_RS20805"
                     /old_locus_tag="pgaptmp_004161"
     CDS             complement(3296..4552)
                     /locus_tag="ACHKYO_RS20805"
                     /old_locus_tag="pgaptmp_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGU4430915.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(4565..4852)
                     /locus_tag="ACHKYO_RS20810"
                     /old_locus_tag="pgaptmp_004162"
     CDS             complement(4565..4852)
                     /locus_tag="ACHKYO_RS20810"
                     /old_locus_tag="pgaptmp_004162"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4430916.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVALTELGAL"
     gene            complement(4868..5311)
                     /locus_tag="ACHKYO_RS20815"
                     /old_locus_tag="pgaptmp_004163"
     CDS             complement(4868..5311)
                     /locus_tag="ACHKYO_RS20815"
                     /old_locus_tag="pgaptmp_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU4430917.1"
                     /translation="MLKNLLNTEVVQVVEQAKDWREAVAISCRPLIENGSIEPRYVDA
                     IYRSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            5582..6613
                     /locus_tag="ACHKYO_RS20820"
                     /old_locus_tag="pgaptmp_004164"
     CDS             5582..6613
                     /locus_tag="ACHKYO_RS20820"
                     /old_locus_tag="pgaptmp_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4430918.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKETEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEITLGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGTETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            7010..7279
                     /locus_tag="ACHKYO_RS20825"
                     /old_locus_tag="pgaptmp_004165"
                     /pseudo
     CDS             7010..7279
                     /locus_tag="ACHKYO_RS20825"
                     /old_locus_tag="pgaptmp_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7510..7608)
                     /locus_tag="ACHKYO_RS20830"
                     /old_locus_tag="pgaptmp_004166"
                     /pseudo
     CDS             complement(7510..7608)
                     /locus_tag="ACHKYO_RS20830"
                     /old_locus_tag="pgaptmp_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase IV"
     regulatory      7729..7917
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            7982..9826
                     /gene="btuB"
                     /locus_tag="ACHKYO_RS20835"
                     /old_locus_tag="pgaptmp_004167"
     CDS             7982..9826
                     /gene="btuB"
                     /locus_tag="ACHKYO_RS20835"
                     /old_locus_tag="pgaptmp_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HGU4430919.1"
                     /translation="MIKKYWFLMVYSVTAFSVWAQDTPLDTLVVTANRFQEPLSTVLA
                     PVTIVTREDIDRWQVSSVNDVLRRLPGVAISQHGGEGQLSTIFVRGTNSNHTLVLIDG
                     VRLNLAGVSGAADLSQFPVALVQRIEYIRGPRSAIYGSDAIGGVINIITSRENTGTEI
                     SAGWGSNSYQHYDISTHQQLGENTRVTLLGDYTYTRGFDAVAYGSTGMQPQSDRDGFL
                     SKTFYGKLEHNLSDTWSGFVRGYGYNNRTKYDAWYSPGSPLIDTRKLYSQSWDAGLRY
                     AGETLQSQLVSSYSHSKDYNYDPHYGRYDTSANLDDMKQYNLQWTNSVTVGHGNVGAG
                     IDWQKQSTTPGTGYLPKGYDQRNTGIYLTGLQKLGDFTLEGAVRNDDNSQFERHTTWQ
                     SSAGWEFIEGYRFIASYGTAFKAPNLGQLYGMYGNPNLAPEKSKQWEGAFEGLTGGVN
                     WRISGYRNDISEMINYNPHTLRYYNDGKVHVKGIEATVNFDTGALTHTVSYDYTDARN
                     ALTDKPLERRPKLQVKYQLDWQVFDFDWGITYQYMGSRYDSDYSSWPYKSVKMGGVSL
                     WDVAVAYPVTPHLIVRGKIANLFNKDYETGYGYQAAGREYILSGSYTF"
     gene            9840..10031
                     /locus_tag="ACHKYO_RS20840"
                     /old_locus_tag="pgaptmp_004168"
                     /pseudo
     CDS             9840..10031
                     /locus_tag="ACHKYO_RS20840"
                     /old_locus_tag="pgaptmp_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10172..12259)
                     /gene="iha"
                     /locus_tag="ACHKYO_RS20845"
                     /old_locus_tag="pgaptmp_004169"
     CDS             complement(10172..12259)
                     /gene="iha"
                     /locus_tag="ACHKYO_RS20845"
                     /old_locus_tag="pgaptmp_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HGU4430920.1"
                     /translation="MRITTLASVVIPCLGFSASSMAAEDVMIVSASGYEKKLTNAAAS
                     VSVISQEELQSSQYHDLAEALRAVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGV
                     RQGGSGDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITKKNA
                     DKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQIRGSTQQRQGSSVTSLS
                     DTSATRIPYPTESQNYNLGARIDWKATEQDVLWFDMDTTRQRYDNQDGQLGSLTGGYD
                     HTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKSDKWGLAGKPREL
                     KESNLILNSLLLTPLGESHLLTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEW
                     HLTDVLALTAGSRYEHHDQFGGHFSPRAYLVWDVTDAWTLKGGVTTGYKAPTMGQLHK
                     GISGVSGQGRTNLLGNPELKPEESVSYEAGVYYDNLSGLNANITGFITDFSNKIVSYS
                     IDDNTNSYTNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTQSEQRDGDNKGAPLSYT
                     PEHMVNAKLNWQITEEVASWLGARYRGKTPRFTRNYSSLSAVQKKVYDEKGEYLKAWT
                     VVDAGLSWKVTDALTLNAAVNNLLNKDYSDVSLYSADKSTLYAGDYFQTGSSTTGYVI
                     PERNYWMSLNYQF"
     gene            12198..12380
                     /locus_tag="ACHKYO_RS20850"
                     /old_locus_tag="pgaptmp_004170"
                     /pseudo
     CDS             12198..12380
                     /locus_tag="ACHKYO_RS20850"
                     /old_locus_tag="pgaptmp_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12800..12993
                     /locus_tag="ACHKYO_RS20855"
                     /old_locus_tag="pgaptmp_004171"
                     /pseudo
     CDS             12800..12993
                     /locus_tag="ACHKYO_RS20855"
                     /old_locus_tag="pgaptmp_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT         3653 a         3147 c         3043 g         3411 t
ORIGIN      
        1 gattagcccg gcaggtgggc gctggctcct gagcgccata aacagggctt tacctgtcag
       61 ctcttttgtc atgcgctctc ccatggcgta gccgacaatt tcgcacgtat aaacatcttt
      121 gatgccagcg aggtacaacc atccctcctg tgtggcaaca tacgtcaggt ccgccaccca
      181 gacctgattt ggtgctgtag gagcgaacgt ctggttcagc agatttggcg caactggcag
      241 attgtggttc gagttcgtag tcgctctgaa cttgcgtttc tgcttacagc gtagccttag
      301 ctccttacga agacgtgcca gtcggtcacg accaacgatg atgccattct ctgccagctc
      361 cgtctggagc cgccgggttc catatgtttc gcgagtgcgg atatgtgcca ccttaatctc
      421 cagttttagc cgctcatcac tttgttttct gtctgagggt tcatgctgta cccagttgta
      481 ataaccgctc ttggatacac caaatacctg acacatcgct tcaatgggaa attgttgtcg
      541 ccattgttcg attaacgcgt atttttcagc gactcctgtg caaaatacgc tgttgtagat
      601 tcaattggtc aacgcaacag ttatgtgaaa acatggggtt gcggaggttt tttgaatgag
      661 acgaacattt acagcagagg aaaaagcctc tgtttttgaa ctatggaaga acggaacagg
      721 cttcagtgaa atagcgaata tcctgggttc aaaacccgga acgatcttca ctatgttaag
      781 ggatactggc ggcataaaac cccatgagcg taagcgggct gtagctcacc tgacactgtc
      841 tgagcgcgag gagatacgag ctggtttgtc agccaaaatg agcattcgtg cgatagctac
      901 tgcgctgaat cgcagtcctt cgacgatctc acgtgaagtt cagcgtaatc ggggcagacg
      961 ctattacaaa gctgttgatg ctaataaccg agccaacaga atggcgaaaa ggccaaaacc
     1021 gtgcttactg gatcaaaatt taccattgcg aaagcttgtt ctggaaaagc tggagatgaa
     1081 atggtctcca gagcaaatat caggatggtt aaggcgaaca aaaccacgtc aaaaaacgct
     1141 gcgaatatca cctgagacaa tttataaaac gctgtacttt cgtagccgtg aagcgctaca
     1201 ccacctgaat atacagcatc tgcgacggtc gcatagcctt cgccatggca ggcgtcatac
     1261 ccgcaaaggc gaaagaggta cgattaacat agtgaacgga acaccaattc acgaacgttc
     1321 ccgaaatatc gataacagac gctctctggg gcattgggag ggcgatttag tctcaggtac
     1381 aaaaaactct catatagcca cacttgtaga ccgaaaatca cgttatacga tcatccttag
     1441 actcaggggc aaagattctg tctcagtaaa tcaggctctt accgacaaat tcctgagttt
     1501 accgtcagaa ctcagaaaat cactgacatg ggacagagga atggaactgg ccagacatct
     1561 agaatttact gtcagcaccg gcgttaaagt ttacttctgc gatcctcaga gtccttggca
     1621 gcggggaaca aatgagaaca caaatgggct aattcggcag tactttccta aaaagacatg
     1681 tcttgcccaa tatactcaac atgaactaga tctggttgct gctcagctaa acaacagacc
     1741 gagaaagaca ctgaagttca aaacaccgaa agagataatt gaaaggggtg ttgcattgac
     1801 agattgaatc tacagttgct ttttttaata tatctcgctc aaggcgagct tcatttaacg
     1861 ccttacgcag ttgcagaatt tcagattcca gttcagccac cgtgcgggaa ccaggagttc
     1921 cgagcccttt tctggcggcg gtaacccatt gtcctaaagt gccttcagga agagataatc
     1981 gggaagcgcc ttcactgatc gaaagttgat tttcaagaac cgttctgaca gcttcggctt
     2041 tgaactcttt agagtaacgt tgggtttttc tgctcattat tagctccttc tgatgccatt
     2101 ctatttcagg aaggagtgtc cgttaaactc aggctacctc attatgtggg atattgcact
     2161 tgctgcccaa tcatcattca gtcagcagaa gcaaaattaa accctgaagc agtcagacac
     2221 ggaatgagtg atcctcgtaa aagaattcag gaggtcagaa tgcgccaata cctgcatata
     2281 gaaagaggcc agctcactgc gtaaatcgtc atggggctaa aatccccgaa taagagtttg
     2341 taaaatccaa gatacagtct gactgtttca ggttctttaa tccgcgatat aacgaagctc
     2401 ccggagctgc gtaaggtctt tttgggctca atatttgaca aaggtcataa gagtttttct
     2461 ttccgagaca gactgcacga agcgctcttt ccgcagcatt attatccgcc tccgccagac
     2521 cgtcatcact gtaataacag agggcatccc actgattcag tacatagctg aacgcttcgc
     2581 ccagtctgga ttttttcgac agcgtaccat tcttctccac catccattca tgcagcgacg
     2641 tcagtaacac tttgcttcgc tgctgcctga cggaaagacg ctctgactcc ggtaatcccc
     2701 gtatttcgta agcgtaaacc gaccgccgta tgtagccatt aagccagtat tggtaacgta
     2761 ggtgcccacc ttttcaaact agtggacgcc tgttatggag caaaaaacat tatccgcaga
     2821 accccgcaga tcattttcaa atgagtttaa acttcaaatg gttaaactgg ctttacaacc
     2881 aaggggttct gttgcccgta tcgcccagga acacgatatc aatgataacc tcctgttcaa
     2941 atagctcaga ctctggcaaa acgaagggcg tatatctcgg cgtcttccgg taacaacctc
     3001 ttctgacact ggagttgaat tattacctgt ggaggtgacg ctggatgagc ggccttaccc
     3061 cgttttttac gcgaggtaat tatgaagatc aatgactagc cgtcgaaccg tgtgcatgaa
     3121 ctgctgcact gaaacttctc agctgtaaaa taatccttac gctacgtact tctcggagcg
     3181 cttacgatta atcggatgca gtcctgactg gaagaaagaa aaatgggaaa aaataagagg
     3241 aattaatatc ctccacgttc ctgaaatcct ggaggatatt aatgtcatca aaacattagc
     3301 gaacgataat ccctaacaaa atacccagtg cgccaaagtc agcatcactg aaggtggtgt
     3361 tagcaaaacc aatatcaccc agcacaggca ataagaatac tggcaggaag gtaataagca
     3421 agccctgagc gaaggcaccc aaaatcgcac cacgacgtcc acccgttgca ttaccgaata
     3481 cccctgcagc tgcacctacg aagaagtgcg gcaccacacc aggaataatc acgatcatgt
     3541 tcagcagata caacgtgaac ataccgatta aacctgcagc aaagctactc aggaaaccaa
     3601 ccagtacagc gttaggtgcg taagggaaga cgaccgggca atcgagagca ggtctggcat
     3661 tcggaaccag cttgtctgaa atacctttga atgccgggac aatctccgca atcaccatgc
     3721 gcacaccctg cagaatgatg tacacgccag ctgcgaaggt aatggattgc atgatagaga
     3781 acatgaacca gtttttacca ccgctaagct ctttcaccgc atctgcaccc gcaaacaggc
     3841 acgtcaccag gaaaattatg ctcatggtaa aagagatagc aaccggcgtg tcgcgcaaga
     3901 acagcagact tttcggcaca ttcatgtctt cagtcgagtg ctctttatta ccaaacttgc
     3961 tgccgatgaa accggccaat acataagata atgttgagaa gtggccgatg gcaacgtcat
     4021 cagaaccggt cactttcttc atgtacggat gcgcaatagc cgggaaaaag accattgcca
     4081 cgccaacaac caaagagcca acggctataa gcgtaatgcc ggtcatgcct gctgttgcca
     4141 gaattaccgc taccatcatc gacataaaca acgtgtgatg accggtcagg aagataaatt
     4201 tccacggtgt gaaacgggca atcataatgt tgataaccat cgcaaagaac ataatcatcg
     4261 ccatttcttt cccgaagctt ttctgcgcta cggaaacaat ggcttcattg ttcggcacga
     4321 caccctgaat accaaaggca tgctggaaaa tgtttgcgaa gtcacccagt gacgacacaa
     4381 ctaaacccgc ccctgcgcct aaaatcacaa accccataat ggtcttgacg gtacctttaa
     4441 tgcattcggt taccggtttt ttctgtgcaa tcaggccaat cagggcgatt aaaccgacca
     4501 gcactgcggg ttcggaaagt acatcgctca tcaaaaaacg aaagaaatcc atatcgcccc
     4561 cagcttacag tgcgcctaat tcggttaatg ccacggataa acgttctctc atggctttct
     4621 tatcaatcat attttccagt gcgacgattt tgcctgcgac agactgcgcg actaactgct
     4681 cggcaatgtc tttggtgccg acatagatat cactcggcgt ccctttcgcg gagcccaggt
     4741 cgacatgatc aacatcagcg ctgacggaca aatccttcaa aatgctttta atgctcattt
     4801 ccatcatcag gctggtgcct aagccgttac cgcaaaccac tgtaattttc ataaatgttc
     4861 ctcaggatta atagcgcgca ataacagaca aaatgtcctg cgtcgttttg gccgtcaaaa
     4921 tagcctgtat gtctttttcg ttatcgaata acttggcgag ttgagaaatc gcttcaatgt
     4981 ggctggtact gtcggttgca gccagcacaa tcagtaattt cactggatcg ttttcatcgg
     5041 catcaaaatt cacacctgaa gggatcaggg tcagtgctaa tgacagcttg ttagctccct
     5101 cttccgggcg cgcatgaggc atcgcaatac cagggccaac cacatagtac ggaccgatag
     5161 tgtcgtgcga gcgatagatc gcatcaacat aacgcggttc gatagaaccg ttttcaatca
     5221 acggccggca tgaaatcgcc acggcttccc gccagtcttt ggcctgttcc acgacctgaa
     5281 cgacctctgt atttaataga ttttttaaca tgcgactgat aactcccgat gataattaac
     5341 aggagagtag atattttacg cattaattaa tgtgatgatt gtcgcatttg atagcgctat
     5401 ctgataacgc taccaatctt tgtgatagcg ctatcatttc tctgtcgcca tgctttggct
     5461 atattcggtt cctgcatata caatgcaaca ggatatttgc taccgtgggc aaaaactgga
     5521 aagagtaatg gtaaaataaa agtaatgaaa acagctggtt gtaattatcc atggagtcga
     5581 aatggaaaaa acacgtaagc gccgtggtac cggacgggtg acgctgcagg aggtcgctaa
     5641 ctttgcaggt gtcggaacca tgaccgtttc ccgcgcgctg agaacaccgg aacaggtttc
     5701 tgacaagctg agggaaaaga tagaacaggc cgtagaagag ttgggttata ttcccaacag
     5761 aactgctggc gcccttgcat ctggtcacag tcatacagtc gccgtactgg ttccttccct
     5821 gacagataaa gccagctccc gctttatgca gtcgttgcag caggtgctta ataaaaatga
     5881 attccagcta ctgcttgggt gtcatgagta taatcagctc aaagaaactg aaattctgat
     5941 gacgcttctt caaggcaacc cggcggcgtt agttattttt ggttctcagt tggctgataa
     6001 aactcaccag atactcgaga aaacaaatat acctaccatt aacgttgtcg gttccccttt
     6061 cagtccggct caaattacca tcgaaacagc attttttgaa gcatcacata aactgaccga
     6121 acatctgctg gagcagggtt ataagaatat cggatttatc ggtgcgcata tggacaaccg
     6181 cctgcaacgt cagcaactga acggctggca taaagcaatg ctgagccatt accagaacac
     6241 tgatcttgtt atcaccatgc cggatgccgc aagtctgcaa ttggggcgtt acgcattaaa
     6301 cgagatatta ctgcgacaac cagagctgga tgctgtgatt tgcagccacg aagagattac
     6361 gctgggtatc atgtttgagt gccagcggcg tttgttaaaa atccctggaa atatcgctgt
     6421 agcttgtctt gacggttcag acaattgcga tcaaacacac ccaactttga catccatccg
     6481 tattgactat aaaaaaatgg ggacagagac gggtaagctg ctgattggac tattgaataa
     6541 taatcatgat gaatcagaag agtcgcgtat cgtgcaattc aactaccaga tagaattacg
     6601 tcagagtaca tgagcctaaa agagcactat tacaaagtta tctattgtgg tagggatagc
     6661 cgtttccggc tattccctgc gcagttccgg atgtatgctg ctaacgcatc cattcctact
     6721 tcaggtattt ggcgtagaag cggatgctaa aaacttgccg caaaaccgca atatcagcct
     6781 gcagtatcgc tctccagatt atccattcct atattgatga caacgacagg taagaccgtg
     6841 acctgataaa cctgaataag ctcctggaca taaagtccgt aatgggaata aggcgagagt
     6901 cagcgaatct catcgaggcc agcagcactg gctgcaataa ggatatagag cagcaatcta
     6961 ccgtcaacgt caaaaacacg gaagccttgc aaacgggatg cgttcatata aacaacatgg
     7021 agcagcaaca atacgatgcc tgtcgttgct cagtcaggac cttttctgtc atgcgcgagc
     7081 cagcgtcacg attgataaat aatgttagca actgattaat ttactgcaac agacgtggtt
     7141 ggtaataagc actggcggac aatgcaggat ggaaggctgt tttcaggttg tccgttcagg
     7201 tacgttagat cagttcgttg cgctaaagac aactgttcag tcccttatgt tacccgttat
     7261 ggcaaccatt gcccggtgat aaccgggcgt gtcatctgtg catccgttga ttcgtgtgga
     7321 ctgataaagt tttgtctgct gaatctttat acggaatgct cttcagtatg cgtaagataa
     7381 tttgtttttt ggaggattgt cgacaatcag agggtaaaca aaagaatggt tgaatgtgga
     7441 tgaggttcat attattgatt cagatttttt gaagaatcac cacatgggat gtccatgtgg
     7501 ttgtataact cagggccagc gcttaataat gatggatgta ttaatgccgc caaaggcaaa
     7561 gttgttactc tgcaaaaaca cacaatcaac cttgcgggct tcatgcaact aattgagcat
     7621 atatgtttta ttcttattat ggtcgccgat aaaatattat gtgtattaga tccgagtgtt
     7681 catgcattcc tatttttgag tcatgtatgg acagtaactt gttacaacca gtaatatcca
     7741 gttgtcggtc ctgcgagtta atagggaatc cagtgcaaat ccgaaactga cacgcagcgg
     7801 taaggaaagg tgcgatgatt gcgtaatgtg gacacggtca tccggtgggt ggtcatctct
     7861 ttgtatcttc agatgccctt ctaggtctga agacctgtcg gttaacgtcg catctggttc
     7921 tcatcatcgc ttactatcga tggtaactgc gatattcttc ttctgttgtg gatgcgttac
     7981 aatgattaaa aaatattggt ttctaatggt gtactccgtc acagcctttt ctgtctgggc
     8041 acaggatacc cctctggata ctctcgttgt tactgctaac cgttttcagg aaccattaag
     8101 taccgtgctt gctcctgtaa ctattgtgac acgagaagat atagatcgct ggcaagtttc
     8161 ttctgtaaat gatgttctgc gtcgtttgcc tggcgttgca atttctcagc atgggggaga
     8221 agggcagctc tcaacaattt ttgttcgtgg gacaaactct aaccacactc tggtattgat
     8281 tgatggagtg cgcctgaatt tagcgggtgt cagtggagct gcagacctga gtcagtttcc
     8341 cgtcgcttta gtgcagcgta tagaatatat ccgtgggcca cgttcagcaa tttatggttc
     8401 agatgccatt ggcggagtga taaatattat tacatcccgt gagaatactg gcactgaaat
     8461 ttccgcagga tggggaagta atagttatca acattacgat atatccactc atcagcaact
     8521 tggggaaaat acccgtgtaa cacttcttgg cgattatacc tatactaggg ggtttgatgc
     8581 tgttgcctat ggcagtacag ggatgcaacc acagtcagac agagacggtt ttttaagtaa
     8641 aacattctat ggcaaacttg aacataactt atctgatacg tggagcggat ttgttcgtgg
     8701 ctacggctat aataaccgaa ctaaatatga cgcctggtac tctcccggtt ctccactgat
     8761 tgatactcga aaactttata gtcaaagctg ggatgcaggt cttcgctacg ctggtgaaac
     8821 actgcaatct caacttgttt ccagttatag ccatagtaaa gattacaact acgatccaca
     8881 ttatggacgc tatgacacat cagctaacct ggatgatatg aaacaataca atctccagtg
     8941 gacaaatagc gtcacagtcg gccacggtaa tgttggcgct ggtatcgact ggcaaaaaca
     9001 atccactact cctggaactg gatatctgcc aaaaggttac gatcagcgta acacggggat
     9061 ttatctgaca ggactgcaaa aacttggcga ttttacgtta gaaggcgcgg tgcgtaatga
     9121 cgacaactcg caatttgaac gccacacaac atggcaatcc agtgcgggat gggagtttat
     9181 tgagggttat cgctttatcg cttcctatgg aactgctttc aaagcgccaa atctggggca
     9241 gctatatggt atgtatggta atccgaatct ggctccggaa aagagtaaac agtgggaagg
     9301 ggcatttgaa ggtttaactg ggggcgtaaa ttggcgtatt tcaggatacc gtaatgatat
     9361 cagtgaaatg attaactata atcctcatac ccttcgttat tacaacgacg gaaaagtaca
     9421 cgttaaagga attgaggcta cagtaaactt tgatacagga gcactgacgc atactgttag
     9481 ttacgattat acggatgcca gaaatgcctt aactgacaaa ccattggaac gtcgtccaaa
     9541 actgcaagtg aagtatcaac ttgactggca agtattcgat tttgactggg ggataactta
     9601 tcaatatatg ggatcgcgat acgattcgga ctactcatcc tggccctata agtcagtcaa
     9661 aatgggcggt gtgagtttat gggatgttgc ggttgcgtat ccggtcactc ctcacctgat
     9721 agttcgcggt aaaatagcta atctgttcaa caaagattat gagactggtt atggctatca
     9781 ggcagcagga cgggaatata tattgtctgg cagctatact ttctgaaatc tgccccaccg
     9841 agtcgctaag cagcgtgatc cgccatgcga tcaaaaagcg taaagtgttc ccgacagacg
     9901 aggcggtgaa aaaagttgtg tggctggcaa tccagtctgc gtcccagaaa tggacgatgc
     9961 cgttgaagga ctggcgtatg gaaatgagcc gctttattat caagttcagt gaccgcctgg
    10021 acggtcactt ctgggaaaag gcatttacac agaatcctga acaggctcgg tcacttcgac
    10081 cagacaccgt gcgtcatgta acgttgccac tgttctgcca ggagttacag tggcaacgta
    10141 tgctaccgtc agtgatggcg ttttgttgtt atcagaactg atagttcagc gacatccagt
    10201 aatttcgctc aggtatcaca tatcccgttg ttgatgatcc cgtctggaag taatcaccgg
    10261 cataaagcgt actcttatcg gcactgtaca ggctcacgtc actgtaatcc ttgttgagca
    10321 ggttattcac cgcagcattc agcgtcaggg catccgtcac cttccacgac agacctgcat
    10381 ccaccaccgt ccaggctttc agatattctc ctttctcatc atacactttc ttctgtacag
    10441 cgctcagcga cgaataattc cgggtgaaac gtggtgtttt cccgcgataa cgggcaccca
    10501 gccatgatgc cacctcttcg gtgatctgcc agttcagttt cgcattcacc atgtgttcag
    10561 gggtataact cagcggtgca cctttgttat caccatcacg ttgttcactt tgggtccagg
    10621 tgtaattcaa cgacagagtg acatcctctg accacagcgg caatgtgccg gcgaattcca
    10681 caccatgcaa gcgggccttt ccgctgtttg tatagctatt ggtgttatca tcaatggaat
    10741 aagagacaat cttgttggag aagtctgtta taaagcctgt tatattggca ttcagaccgg
    10801 agaggttatc gtaatatacc cccgcctcat aacttacact ctcttccggc ttcagttcgg
    10861 ggttaccgag tagattagta cgtccctgcc cggatacacc gctgatcccc ttatgtaact
    10921 gtcccatggt gggggcttta tatcccgtgg tcacaccgcc tttcagcgtc caggcatcgg
    10981 tcacgtccca gaccagatat gcacgcggac tgaagtgtcc cccgaattga tcatgatgtt
    11041 catagcgact gcctgcagtc agcgcaagta catccgtgag atgccactca tcctcagcaa
    11101 atactgacca gcttttctgt cggaaagttt cacctgtgct ggcaaggaca accccatctt
    11161 tcatggacga actctgatac tctcccccca cagtaagcag atgagattct cccagagggg
    11221 taagcagcaa tgaattcagg ataaggttag attccttaag ctcccgcggt ttaccggcaa
    11281 gcccccattt atcgctcttc agaacactac gtacaagctc acgaccttta ttttccgtct
    11341 cgttccagtt cagatacgat ttccaggtac cgaaggtgaa agtatgatca tagccagccg
    11401 aaattttgtt tcgctcatag cgcagagtat ggtcatatcc ccctgtcagg ctacccagtt
    11461 gaccatcctg gttatcgtaa cgctgtcggg tggtatccat atcaaaccag agcacatcct
    11521 gctccgtagc cttccagtca atacgcgcac caagattata attctgtgac tccgtgggat
    11581 aaggaatacg cgtggctgat gtgtcgctca gtgatgtgac cgatgagccc tgacgctgct
    11641 gtgtgctgcc gcggatctgc agactgacag aatcatccac aagagggcca ctgctccaga
    11701 aattaaactg gctgctgttg ccccatttgt tactctcctg cagattcagc ccggcgttga
    11761 cggaagagag ccatttgtct gcattctttt tggtaatgat attcaccaca ccacccatcg
    11821 catcagagcc atacagtgtg gacatcggcc ccctgataac ctcaatacgc tcaatcgcgg
    11881 ccagaggagg catgaaccct gtattcatgg cagaaaaacc gttaggcgtc acgtccccgc
    11941 tcccgccctg acggacacca tcaatcagta tcagcgtgta actggcgggc attccacgga
    12001 tgctgatttc cagcccaccg gttttacccg taccactttc aacatccaca ccctccacag
    12061 ctctcaatgc ctccgccaga tcgtggtact ggctggactg caattcctcc tgactgatta
    12121 cggaaacact ggcggctgcg ttggtcagtt ttttctcata gccggatgcc gagacaatca
    12181 tcacatcctc tgctgccatg ctgctggctg aaaacccgag acagggaatg acaacggaag
    12241 ccagagtggt tattcgcata ttactaatct ccgatcatgg taatcggcaa gacaatacat
    12301 aaatacaaac aataatgaga ataatttata tttacctttt acaaaaaatg aatattactg
    12361 acaaaactaa atataaataa tgactgcctc ggcatgccag ttaacagcca tccgcaatgg
    12421 cgtgttcgat tttgcgaata aaaaatggtt actgtagaag cggattttca ccacctgtca
    12481 accatgaatg gtcagtggag tgagacgact ggtatgattt ggtcgatgac ttcttccggt
    12541 gaatacggac agaaacattg aaatattgta agagagaacg ttcagaattc tgtgtcacgt
    12601 gaaaatacta cagcgttatc accttattca gtcgaccttc aaaataaacg tctaaccgtg
    12661 acaatcacca tttggattgc ccactggaga ggcgaacgat tgcctcgacg gcatttataa
    12721 tgccagccca cattgttggc gctgacaata ctaaggacaa acgatgaggg agaaccgtct
    12781 tataccacta gcgctgacac cggcacacct tccccgtgaa atacaccggc tggagcctga
    12841 agaaagctgt tgcccggagt gtggcggtga gctggattat ctggggaagt cagcgctgaa
    12901 caactggaac tggtgagcag ctccctgaaa gtgatcagca cagaacgggt aaaaaaagcc
    12961 tgtaaaaaat gtgacggtac cgttgaagcg ccggtctcgt aagaataatc ctgggcaaat
    13021 aagatttttc ctgaaaaagc gtctagtctt ttgatatcgt tgtttttatg ctaatcaggg
    13081 agtatatgtg atgattgtct gttacgttat tcttgttaac cgtccgtttg aaacagagat
    13141 tgccgggaaa aagctatttt ttcagaaaaa tgacgtcctg ttgacagata acatcagcca
    13201 gtcctttttc ctgcgccatc ccggtaatgt tactgagctc cggattgacg aatg
//
LOCUS       DAZGAN020000131        13167 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGAN020000131 DAZGAN020000000
VERSION     DAZGAN020000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.131"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..181
                     /locus_tag="ACHKYO_RS20860"
                     /old_locus_tag="pgaptmp_004172"
     CDS             <1..181
                     /locus_tag="ACHKYO_RS20860"
                     /old_locus_tag="pgaptmp_004172"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430921.1"
                     /translation="LPGGLMADDDKIIYRMIYFSACKYQARIYYAINDKYHIICGFPS
                     TQVRFPAKPEPHFSA"
     gene            complement(237..1259)
                     /locus_tag="ACHKYO_RS20865"
                     /old_locus_tag="pgaptmp_004173"
     CDS             complement(237..1259)
                     /locus_tag="ACHKYO_RS20865"
                     /old_locus_tag="pgaptmp_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HGU4430922.1"
                     /translation="MDKRVIFAVAGSGKTTLLIRRLREDRRTLILTFTVNNEAHLRAQ
                     IIRRFGYIPYGIRVMTWFEFLHGFCFRPFLQEQLSSRGLSFNQPPSRIPRTNIRHYQD
                     PAGRLYHRRLAHLLTARGLLPDIRTRLARYYDELFVDEVQDFAGHDFNFLLELCRAEI
                     SVLCCGDFYQHTFDTSRDGNVNATLHEDITRYEARFRAAGIMVDCETLSRTWRCSATV
                     CEFITGQLNIRISAHGTHTTQIEIVTDEARSAALHADNTMIKLFYREHHRYGCHSMNW
                     GGSKGLDHFQDVCIVMGANHWMRLIQQKLAALPPSSRNRLYVACSRARGNIYFIPESH
                     LRRFRN"
     gene            complement(1244..2809)
                     /locus_tag="ACHKYO_RS20870"
                     /old_locus_tag="pgaptmp_004174"
     CDS             complement(1244..2809)
                     /locus_tag="ACHKYO_RS20870"
                     /old_locus_tag="pgaptmp_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4430923.1"
                     /translation="MPLITRLMLQNFKKFPELDLRFSNDRNILVGDNESGKSTILLAL
                     DLVLSDSRHRVEALGVESLLSQSAVRHFQEGERRADQLPVLTADVFLSNGGEPDLNGR
                     QNLAGIDADGLRMRIAPMMEEYGQDIHHVLQQDPDNFPYEYYSVQFSTFSGGHFAGFR
                     RYLRHLFLDSARIDNEHAAQEYTRTVYSVNVPVADRYRLENSYRQQKMHFCARHLSAI
                     NDTLKTYQFGVRSGAKSGLEANLDITEDGISIRHRGKGRQCFIKTEFALQRHQQQGGE
                     IHALLLEEPENHLSHVSMKRLVNQLATERQTQVFIATHSSHISSRLDLRKAILLGATR
                     PVLMNELSAETAAFFMKAPDNNVLEFALARRVLLVEGDAEFILIEAFYHRLYGRAPED
                     DGVHIIAIGGTSFRRYLELARLLENRVAALRDNDGNYQQNCDERYADVLCSRSRVFAD
                     HDNSRSTFEICLYQDNADLCDALFRGTRRTLTVQDYMLANKAEAAFQLLQLHAEKLTV
                     PDYIQEALAWIRE"
     gene            complement(2884..3300)
                     /locus_tag="ACHKYO_RS20875"
                     /old_locus_tag="pgaptmp_004175"
     CDS             complement(2884..3300)
                     /locus_tag="ACHKYO_RS20875"
                     /old_locus_tag="pgaptmp_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4430924.1"
                     /translation="MNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRAAVDATTEIKVALRLAGTPIGPN
                     DTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR"
     gene            complement(3297..3527)
                     /gene="vapB"
                     /locus_tag="ACHKYO_RS20880"
                     /old_locus_tag="pgaptmp_004176"
     CDS             complement(3297..3527)
                     /gene="vapB"
                     /locus_tag="ACHKYO_RS20880"
                     /old_locus_tag="pgaptmp_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HGU4430925.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLELEKADPDFMTEREDVVSDEGRVNL"
     gene            3908..7702
                     /locus_tag="ACHKYO_RS20885"
                     /old_locus_tag="pgaptmp_004177"
     CDS             3908..7702
                     /locus_tag="ACHKYO_RS20885"
                     /old_locus_tag="pgaptmp_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pcar"
                     /protein_id="HGU4430926.1"
                     /translation="MPVLSANKAQEEQINTSYEPLTPSVITDEKAQSYIEALNFACSR
                     PDIRNIAVTGPYGAGKSSVLLTWERAEDNDFRVMTVSLADFEMQRAYPGDSLSVEGKP
                     DYDDNEKKAGKAEEKTIEYSILQQLLYKEKKSVLPYSRLERISDVSACQIAMMTASLL
                     FILASTATGLLFLFPDYIREKLSLSPELSQFLLELPVLARFGSAGIFLFTALFFALKK
                     LHRTGVFDRRVSIDKVDILKGAITTRPAAPSLLNVYIDEIVYFFEQTQYNVVIFEDLD
                     RHNDGAIFIKLREINQLINNCLPTDNPVRFIYAVRDNLFITPESRTKFFDFVIPVIPV
                     MDSENASEHFLSKFTLDELRQEGFKDCLARLALFIPDMRVMHNIANEFRLYRNIVNNG
                     GDLKRLISLITYKNLYAEDYHRIDQKKGMLYSIVSEYTSGKLREDYCNNLKNKIETCL
                     TELSKLHNEEVVTEHELRSEILRPYISEKTAPRLHIMTGTGGQYDFDDVIQNENTFLS
                     LLSNQIISIKPSGYNITITTIDKKTAQVMKEEYQERIAIIQKKLNNDVSRLEENIKKN
                     RCEIQNASSYDLAFFINKMGRSGFERYIACCSTPEQHDGESITDNAANIDFIYFLLSH
                     GYLSTDYMAYRSVFMPGSLSTEDNNFIRAVTSGRLPDETAKMPLSNIANTVAKLHGLG
                     ILMHDNAWHPQILWYLMRNDTNSLKTIMRMQAEVGAERRMVRLANEIFPLWEPAAQRE
                     YIRLMVDGDGHLSTMIHQIGRLNDTVAEQNLLPVLLSLPILSWEAVSQITREELQRLI
                     DLQFNLVTSLPENCAQFFCENLRNSGCRLTNIPLARSDSGQETLHLVVQKKLWTYSTL
                     NLQNICFSLSHESENNSDTFRKKPVALIKSLRIPNLEKYVYENISSFIRDVFIHSEEN
                     DLIPDFLNSTFVDWDDAKYMTESMSFVLEDVSVILNKENTETTEISYDQNLYSLLAHH
                     NHITPCWNNVISLLSEDASIAGDTFCEWLNINYSLLPNDSLPLTDVQFSQLLIKAVTS
                     PHISKEALIAITMAFRITLINVPENLPLNNAAVLIKQKWLAPTSTVFEQLYQALYEEG
                     DKLTSLLYALICARPVLLSDNYELVLFSDDQFDLGITRLILNGDKIADEVCISILNWL
                     WEKDEALLSEAPLLSQQALIRFSTKITDDRQKQALLMQCLKNDGGSHKFIRQVLMTFG
                     HQDYAAFLTERNYRSIPRSDAMWQLAVQLGNSGFIRPPKLTHADTRIRIEPFFNAENE
                     YD"
     gene            complement(7747..8163)
                     /locus_tag="ACHKYO_RS20890"
                     /old_locus_tag="pgaptmp_004178"
     CDS             complement(7747..8163)
                     /locus_tag="ACHKYO_RS20890"
                     /old_locus_tag="pgaptmp_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4430927.1"
                     /translation="MNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRAAVDATTEVKVALRLAGTPIGPN
                     DTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG"
     gene            complement(8160..8390)
                     /gene="vapB"
                     /locus_tag="ACHKYO_RS20895"
                     /old_locus_tag="pgaptmp_004179"
     CDS             complement(8160..8390)
                     /gene="vapB"
                     /locus_tag="ACHKYO_RS20895"
                     /old_locus_tag="pgaptmp_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HGU4430928.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLR
                     PSWSSFLELEKADPGFMTEREDVVCDEGRFNL"
     gene            8655..9155
                     /locus_tag="ACHKYO_RS20900"
                     /old_locus_tag="pgaptmp_004180"
     CDS             8655..9155
                     /locus_tag="ACHKYO_RS20900"
                     /old_locus_tag="pgaptmp_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN family nuclease"
                     /protein_id="HGU4430929.1"
                     /translation="MGNYSDFETDFVQRTLALIDQYNEMIEVLGKPFREQYNYTLTLN
                     CLLGLIVLPKERALSFLPADRLTRQLKAEMGLHESQLPGPEMNLRELIHKMRNSVAHF
                     CVQVESASDAHLVDWIVFRESQEDGEVYASFSAPELLPFLKYYATLLLDNMARRRAPD
                     VNILDL"
     gene            9168..9941
                     /locus_tag="ACHKYO_RS20905"
                     /old_locus_tag="pgaptmp_004181"
     CDS             9168..9941
                     /locus_tag="ACHKYO_RS20905"
                     /old_locus_tag="pgaptmp_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430930.1"
                     /translation="MLILDCSSRTQALHTLSAGFGCSPEKLKKVLLSLDLESIYELNP
                     RQLVDAPQYLREYVCAELGEPGPFTRALWFHGTRTFAGNTFPAGLLALNQSESLAMKM
                     LLDLAPNEMVRTHLKEWDVPGGVPDEMFQLRTGDKIHWGPFGHLVRELHFNASENGLH
                     DYLWLPELVEDVCKAYQKKYGHDLKPHYLSVLHPCIVWFEADIVYEKGVLETALSYAY
                     TSVRDLPPDGNATFGIDCDGKSVSRSAIARIEFLQPGQM"
     gene            10108..11241
                     /locus_tag="ACHKYO_RS20910"
                     /old_locus_tag="pgaptmp_004182"
                     /pseudo
     CDS             10108..11241
                     /locus_tag="ACHKYO_RS20910"
                     /old_locus_tag="pgaptmp_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
     gene            complement(11275..11787)
                     /locus_tag="ACHKYO_RS20915"
                     /old_locus_tag="pgaptmp_004183"
     CDS             complement(11275..11787)
                     /locus_tag="ACHKYO_RS20915"
                     /old_locus_tag="pgaptmp_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430931.1"
                     /translation="MTIFKENIVDTSLIGPILTALLAAAGFYCKFWFTRYQASRDQAQ
                     KVSRAIEDVLSKMAALFGQKKPARILREEFSAVIAPLHQEMRILDDMTSRLPLKWLQR
                     EQRHVLYHARWLQRYLDSRRGGSDGDFFLLLHDVAMGTDYQVADILASQHDCKQRRGS
                     QLPVNLKTCP"
     gene            12355..12573
                     /gene="ccdA"
                     /locus_tag="ACHKYO_RS20920"
                     /old_locus_tag="pgaptmp_004184"
     CDS             12355..12573
                     /gene="ccdA"
                     /locus_tag="ACHKYO_RS20920"
                     /old_locus_tag="pgaptmp_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HGU4430932.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            12575..12880
                     /gene="ccdB"
                     /locus_tag="ACHKYO_RS20925"
                     /old_locus_tag="pgaptmp_004185"
     CDS             12575..12880
                     /gene="ccdB"
                     /locus_tag="ACHKYO_RS20925"
                     /old_locus_tag="pgaptmp_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HGU4430933.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            12881..>13167
                     /locus_tag="ACHKYO_RS20930"
                     /old_locus_tag="pgaptmp_004186"
     CDS             12881..>13167
                     /locus_tag="ACHKYO_RS20930"
                     /old_locus_tag="pgaptmp_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4430934.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSL"
BASE COUNT         3354 a         3187 c         3239 g         3387 t
ORIGIN      
        1 gcttcctggg gggttaatgg ctgatgacga taaaatcata tatcgaatga tttatttctc
       61 tgcctgtaaa tatcaggcca ggatttatta tgcgattaat gataaatatc atatcatctg
      121 cgggtttccc tccacgcagg tccggtttcc ggcgaaaccg gagcctcatt tttcggcctg
      181 aagttttgtc gttgactact ttttaccggc ttgtgcccag ttgagaagcc gctgacttag
      241 tttctgaatc tccgaagatg gctttccgga ataaaataga tgttgccccg tgcccgtgag
      301 caggccacgt atagcctgtt acgggatgat ggtggaagtg ctgccagctt ctgttggatg
      361 aggcgcatcc agtggttggc ccccatcacg atgcagacgt cctgaaagtg gtcgagtcct
      421 ttactgcccc cccagttcat tgagtggcat ccgtaacggt gatgttcgcg atagaagagc
      481 ttgatcatcg tgttgtccgc gtgcagggcc gctgaacgcg cttcatccgt gacgatttca
      541 atctgggtgg tatgtgttcc atgagccgaa atgcgaatgt tcagttgtcc ggtaatgaac
      601 tcacacactg tagctgaaca gcgccatgtc cggctgagcg tctcacaatc caccatgata
      661 cccgcagccc tgaagcgagc ctcgtatcgg gttatgtctt catgcagcgt agcattgacg
      721 ttaccgtcgc ggcttgtgtc aaaggtatgc tgataaaaat ccccgcagca cagcactgag
      781 atttccgcgc ggcatagctc cagcagaaaa ttaaagtcat ggccggcaaa gtcctggact
      841 tcatcaacga acaactcatc gtagtaacgg gcaagtcggg tacggatatc tggcagcaac
      901 ccccgggctg tcagcaggtg tgcgagccgc cggtgataca gccgtccggc gggatcctga
      961 taatgccgga tattcgtgcg cggtattctg gaagggggct ggttgaagct cagaccacgc
     1021 gatgacagtt gctcctgcag gaaagggcga aaacagaaac cgtgcagaaa ctcaaaccag
     1081 gtcatcacac ggatcccgta cggtatgtag ccaaagcgtc tgataatctg cgcccgcaga
     1141 tgtgcttcat tatttacggt gaaggtcagg atcagcgtcc gccggtcctc tcttaaccga
     1201 cgtatcagca gcgtggtttt acccgagccg gcaaccgcaa atatcactct cttatccatg
     1261 ccagtgcctc ctggatataa tcaggaacgg tcagcttttc ggcgtgcaac tgtaacagct
     1321 ggaatgctgc ttctgcctta ttggccagca tgtaatcctg aaccgtgagt gtccggcggg
     1381 ttccgcggaa aagcgcatca cacaggtcgg cattatcctg atacaggcag atttcaaatg
     1441 tggagcggct gttgtcgtgg tcagcaaata cccgggagcg ggaacaaagc acgtcagcat
     1501 agcgttcatc gcaattttgc tgatagttgc cgtcattgtc gcgaagggcc gcgacacgat
     1561 tttccagaag acgggccagt tcaagataac gacggaaact cgttccgccg atagcgatga
     1621 tgtgaacccc gtcatcctcc ggtgccctgc catacaggcg gtgataaaag gcctcgatca
     1681 gaataaactc agcatccccc tccacgagca gcacgcgtct ggccagcgca aattccagca
     1741 cgttattatc cggtgctttc atgaagaagg cggcagtctc agcggaaagt tcattcatca
     1801 gtaccggccg ggtagctccg aggagaatag ccttacgtag gtcgaggcgg gaggagatat
     1861 ggctactgtg tgtggcaata aacacctgcg tctgccgttc cgtggcaagc tgattaacca
     1921 gcctcttcat actgacatgg ctgagatgat tctccggctc ctcaagcaac agcgcgtgga
     1981 tctctccccc ctgctgctga tgccgctgca gcgcaaactc cgttttgatg aaacactgtc
     2041 ttccttttcc gcggtgacga atggaaatac cgtcctcagt aatgtccagg ttcgcctcaa
     2101 gacctgattt agcgccggaa cgcacgccaa actgatacgt tttcagcgtg tcgtttatgg
     2161 cggacagatg ccgggcgcag aagtgcattt tctgttgccg gtaactgttt tcaagccggt
     2221 accgatcggc caccggcaca ttgacgctgt aaactgtgcg cgtatactcc tgtgcggcat
     2281 gctcgttatc aatgcgtgca ctgtctaaga acagatggcg cagatagcgt ctgaagcctg
     2341 cgaaatgacc accggagaat gtgctgaact gcacggaata gtattcatag ggaaaattat
     2401 caggatcctg ctgcaaaaca tggtgtatat cctgaccgta ttcctccatc atgggagcaa
     2461 tgcgcattct gagaccatcc gcatcgatac ccgccagatt ttgcctgccg ttcaggtcag
     2521 gctctccccc attactcagg aaaacgtcag cggtcagcac gggtaactga tcggcacggc
     2581 gctcaccttc ctgaaaatgc ctgactgctg actgtgacag gagcgattcc acccccagcg
     2641 cctcaacccg atgcctgctg tcactcagca caaggtcaag agccagcaga atagtgcttt
     2701 tcccggactc attgtcccca accagaatgt tacgatcgtt gctgaagcgc aggtccagtt
     2761 ccggaaactt tttgaagttt tgcagcataa gccgcgtgat cagtggcatt tttttattcc
     2821 tttatatacc gctgcctgaa agagcggccg gccgcgcctg accgcctgtt ttgttttaca
     2881 gcgttatctt acccagtctt ccagcacaag acccggtacc cgctcaaact ctctcacatt
     2941 attcgtcacc agaatggcac aggcagcgat ggcgtgtccg gctatcgccg tgtcgttcgg
     3001 accgatcggc gtgccggcga gacgcagtgc caccttaatc tccgtggtcg catccaccgc
     3061 ggcgcggtcc cacggcagga cggcatcgag cctctcacag aacgcgtcaa ccagttgcac
     3121 gtggcgtggc gaggccttcg gaccggtggc accgaagcgc atctcggagt aggtgatggc
     3181 cgagaccacg atacggtgac cgcgcagcac cgactgctcc aggtgcttaa gcaatgcttc
     3241 aggttgctcg cgcatgatga acgagcagat gttcgtgtcg agcatataga tcttgttcac
     3301 aggttaactc gtccttcgtc gctgacaacg tcctcgcgct ccgtcataaa gtccggatcg
     3361 gccttttcta gctccaggaa tgagccccag gtcggccgga cggggcgcag aatgatgctg
     3421 tccccttccc ggacgatctc cagctcgctc accccctcaa aatccagatc gcgtggaagg
     3481 cggatagcac ggttgttgcc atttttaaaa atggatacgg ttctcatgat tctctcccgg
     3541 gtaagtgtgg atatgtgatg catatccgga atgatgttca ttgcaccggg tgtgtgcata
     3601 tgtttagtat aggcagatgt taggcatatg tatagattca gcatatgcag atggtgagga
     3661 tatgcgtata ccatgaatat gcagatgtca ggcatataga tatgttcagc ctatgttatt
     3721 ttctctcttt ccccgtgcag atccggtttc tggtgaagtc ggagcccacc atctccgagt
     3781 atttgataag ccaattcttg tatccctttg cctttattat ctttttggtt ttcagaagtt
     3841 ataatgcctg ttaaatcaac aatgttggaa gatcaacatg agaagaccat cccgaactga
     3901 agcactgatg ccagtactga gtgctaacaa agctcaggaa gaacagatta acacctccta
     3961 tgaaccactg actccctcag tcattacaga tgaaaaagca caaagctata tcgaagcttt
     4021 gaattttgcc tgttctcgcc cggatatcag aaatattgca gtaacagggc cgtacggggc
     4081 cggtaaaagt tcggtattac tgacatggga aagggcggag gataatgact ttcgggtgat
     4141 gaccgtatca ctggctgact ttgaaatgca gcgggcatac cccggagatt cgctgtcagt
     4201 tgagggtaag cccgactatg atgataacga aaaaaaagcg ggaaaagccg aagagaaaac
     4261 aatcgagtac agcatcctgc aacaactgct ctataaagag aaaaaaagtg tgttacccta
     4321 ttcccgtctg gagcgcattt ctgacgtaag tgcgtgtcag atagccatga tgacggccag
     4381 tctgttattc atactggctt cgacggcaac tggtctgctt tttcttttcc cggattacat
     4441 ccgggaaaag ctctccctgt cgccagaact gagtcagttt ctgctggagt tgcctgtact
     4501 cgctaggttt ggtagcgccg ggatatttct ttttactgct ttattctttg cactgaagaa
     4561 attgcaccgt acaggggtat ttgatcggcg tgtaagtatt gataaagttg acatactgaa
     4621 aggtgccatt acaacacgcc ctgcggcccc ttctcttctc aatgtgtaca ttgatgaaat
     4681 cgtctacttt ttcgaacaga cacaatacaa tgtagtcata tttgaggatc ttgaccgtca
     4741 taatgacgga gccattttta tcaaactccg ggaaataaat cagcttatca ataactgcct
     4801 ccccacagac aatcctgtcc gatttatcta cgctgtcagg gataatcttt tcataacacc
     4861 agaatcacgg acaaaatttt tcgatttcgt cataccagta atcccagtaa tggacagtga
     4921 gaatgcttct gagcattttc taagtaaatt tacacttgat gaactcaggc aggaaggatt
     4981 caaagactgc cttgccagac tggctctttt tattcctgat atgcgggtta tgcataacat
     5041 tgcgaatgaa tttcgtcttt accggaatat tgtgaataat ggaggagatt taaagcgtct
     5101 tatttcactg atcacctata aaaaccttta tgctgaagac tatcacagga tagatcagaa
     5161 aaaaggaatg ctttatagca ttgtatctga atatacatca ggtaaattaa gagaggatta
     5221 ttgcaataat ctaaaaaaca aaattgaaac gtgtctaact gaactatcta aactacataa
     5281 tgaagaagtg gtcactgaac atgagttaag aagtgaaatt ttgcggccat atatcagtga
     5341 aaaaacagca ccccgattac atattatgac aggtacagga gggcaatatg attttgatga
     5401 tgtaatccaa aatgaaaata catttctttc tttgctgagc aaccaaataa tcagcattaa
     5461 acctagtgga tataacatta ctataacaac aatcgataaa aaaacagctc aggttatgaa
     5521 ggaggaatat caggaaagaa tagcgattat tcaaaagaaa ctaaataatg atgtttctcg
     5581 attggaggaa aatattaaaa aaaacaggtg cgagattcag aatgcatcct cgtatgatct
     5641 tgcttttttc atcaataaaa tggggagatc aggtttcgaa agatatattg catgttgttc
     5701 aacgcctgaa caacatgacg gagaatccat tactgataat gccgcaaata ttgattttat
     5761 ttatttcctt ctatctcatg gttatctttc cactgactat atggcctacc gctcagtctt
     5821 tatgccaggt agtctgagca cggaagacaa taactttatc agggctgtga cgtcaggaag
     5881 attacctgat gaaacggcaa aaatgcccct cagcaatatt gctaacacag tagcaaaatt
     5941 gcatggactg ggcatactga tgcatgacaa tgcatggcat cctcagattc tctggtacct
     6001 catgcgcaat gatacgaact ctctgaagac tatcatgagg atgcaggcag aggtcggagc
     6061 cgaacggcgg atggtaagac tggccaatga gatcttcccc ctatgggaac ctgctgcgca
     6121 gcgggaatat atcagattaa tggtcgacgg ggatggtcat ttatccacta tgatccacca
     6181 gattgggcgc ctgaatgata ccgtagctga acaaaatctt ctcccggtat tactgtcctt
     6241 accgatactt tcttgggaag ccgtctctca gataactcgg gaagaactgc agagactcat
     6301 cgatttgcaa tttaatctcg taacgtcctt accggagaat tgtgcgcagt ttttctgtga
     6361 aaacctgcgg aattcaggtt gtcgcctgac taacattcct ttggcccgga gtgactctgg
     6421 gcaggaaaca ttgcatttgg tggtacagaa aaaattgtgg acctattcca cgttaaacct
     6481 gcaaaacatt tgtttctcac tttctcatga gtcagagaat aacagtgata ccttccgtaa
     6541 gaagcctgtt gctttaataa aatcgcttcg tattccgaac cttgaaaaat atgtttatga
     6601 aaacatatca tcattcattc gtgatgtttt tatacactct gaagaaaatg atctcattcc
     6661 tgattttctt aattcgacat tcgttgactg ggatgatgca aagtacatga cagaaagtat
     6721 gagcttcgta ctggaagacg tatcagttat cctgaacaaa gagaataccg aaacaacaga
     6781 aatttcttat gatcaaaacc tgtattcact tctggctcat cataatcata ttaccccttg
     6841 ctggaataat gttatttctc tactgagtga agatgcgtca attgctggtg atactttttg
     6901 tgagtggctt aatattaatt attctttatt gccaaatgac tcgttgcctt tgactgatgt
     6961 tcagttctcg caattactta ttaaggctgt tacatcacct catatcagta aagaagcgtt
     7021 aatagcgata accatggcat ttaggataac tcttattaat gtgccagaaa acttaccact
     7081 gaataatgct gcggtactaa tcaagcaaaa atggctggca ccaaccagta ctgtgtttga
     7141 acaactttat caggcactgt acgaagaagg tgacaaactg acatcactac tttatgcact
     7201 tatttgtgcc cgtcctgtat tattgagtga taattacgag cttgttttat tttctgatga
     7261 ccagtttgac ctggggatta ctcgtttaat tctgaatggc gataagattg cagatgaagt
     7321 ctgtatcagt atcctgaact ggctctggga aaaagatgaa gccctgctct ctgaggcacc
     7381 acttctcagc cagcaagccc tgataaggtt ctccaccaaa ataaccgatg accggcagaa
     7441 gcaggcactg ctgatgcaat gtcttaaaaa tgacggtggg tcacacaagt tcatcagaca
     7501 ggtgctcatg acgttcgggc atcaggatta tgccgctttc ctcacagaga ggaattatcg
     7561 tagcattccc cgtagtgatg ccatgtggca actggctgtc cagctgggaa atagtggatt
     7621 tatcaggccg ccgaaactta ctcacgctga tacacgtatt cgtatagagc ccttttttaa
     7681 cgctgaaaat gaatacgact gacatcgcaa aatagtggta agtccgtcgg gggcggcctt
     7741 actcctttat cctgcccagt cttccagtac cagtccaggc acccgctcaa actctctcac
     7801 attattcgtc accagtattg ccccggtggc gatggcgtgc ccggcgatcg ccgtgtcgtt
     7861 tggaccgatc ggcgtgccgg cgaggcgcag cgccacctta acctccgtgg ttgcatccac
     7921 cgcagcgcga tcccagggca ggatggcatc gagacgggcg cagaacgcgt caaccagctg
     7981 cacgtggcgt ggcgatgcct ttggaccggt ggcgccgaag cgcatctcgg agtaggtgat
     8041 ggccgagacc acgatacggt gaccgcgcag caccgcctgc tccagattct taagcactgc
     8101 ttcaggctgc tcgcgcatga tgaacgagca gatgttcgtg tcgagcatat agatcttgtt
     8161 cacaggttaa atcgtccttc gtcgcagaca acgtcctcgc gttccgtcat aaagcccgga
     8221 tcggcctttt ctagctccag gaatgagctc cagctcgggc ggaggggacg cagaatgatt
     8281 ctgtcccctt cccgtacaat ctccagctca ctcaccccct caaaatccag atcgcggggc
     8341 agacggatcg cgcggttatt gccatttttg aaaatagata cggttctcat gtcttcctcc
     8401 tggcaaggcg gcttttatat atgtttagta tatgcagatg tgatacatat gtatatgtta
     8461 taaatatgca gatgtgtgag atatgcgtat gcctgtcata tgcagatgcc aaacatatgg
     8521 atatgcatgg tatctgcatg tttctctctt ttttcaccgg gatccgaaaa atatcatagt
     8581 aggaaatgcc gtacctgagc cgtcaggctg gccggatgat atagtggatt ctcgaaccgg
     8641 aacgggaggg ttaaatggga aattattcag attttgagac cgattttgtt cagcgaacac
     8701 tggcgcttat cgatcaatat aacgaaatga ttgaggtgct gggcaaacca ttcagggagc
     8761 agtacaacta cacgttgacc ctgaattgcc tcctgggtct tatcgtgtta ccgaaagaga
     8821 gagcgctctc tttcctgcct gccgaccgtt taacgcggca acttaaagct gaaatgggat
     8881 tacatgagtc acagctgccc ggaccggaaa tgaacctgcg ggagttgatc cataagatgc
     8941 ggaattcagt tgctcatttc tgcgtccagg tagagtcagc cagcgatgca caccttgtag
     9001 actggattgt tttcagggaa tctcaggaag atggagaagt ctacgccagc ttcagcgctc
     9061 cggagctttt accctttctg aaatattatg cgaccctgct gctcgacaat atggcccgcc
     9121 gtcgagcccc cgatgttaac atattggatt tgtaagggat ttacaccatg ttgattctcg
     9181 attgctcctc acgaactcag gcgttgcata cattatctgc cgggtttggc tgttcccctg
     9241 aaaagctgaa aaaagtgctg ctttcgctcg atcttgaaag tatctacgag cttaatcctc
     9301 gccagttggt tgatgcacct cagtatctga gagaatacgt ctgtgcggaa ctgggcgaac
     9361 cgggaccatt cacccgggct ctgtggtttc atggcacccg gactttcgcc ggtaatactt
     9421 ttccggcggg gttactggcg ctaaatcagt cagaatccct cgccatgaaa atgctgcttg
     9481 acctggcccc gaatgagatg gtccgtacac acctgaagga atgggatgtt ccggggggcg
     9541 ttccggatga gatgtttcag ctcagaaccg gcgataaaat ccactggggg ccttttggcc
     9601 atttagtccg ggaattacat tttaacgcca gtgaaaatgg actgcatgat tacctctggc
     9661 tgccggagct ggtagaggat gtctgtaagg cctaccagaa gaaatacggc cacgatctta
     9721 aaccgcatta tctgagcgtg cttcatccct gtattgtctg gttcgaggcg gatattgttt
     9781 atgaaaaagg agttcttgaa acggctctgt catatgccta tacatcagtc cgtgacctcc
     9841 cgccggacgg aaacgcaact ttcggcatag actgtgacgg gaaatctgtg tctaggtcgg
     9901 ccattgccag aatcgagttt ctgcagcccg ggcagatgta atccctaaaa aacgggtctg
     9961 tgatccggcc cgtttgttca ttgccgtgaa cccagtattg aggtcattaa agccttaagt
    10021 ggatcacttg cagctggctg cagaaaggtt ttacattgac ccggattatg ccgtttccct
    10081 tcctcctgcc tggtaaagga tctctgaatg cacttttatg tggatgaaac aggccagact
    10141 ggccgaaacc tttttgacaa aacctagcca gtcctctcct acggagtgct gtcgtctgat
    10201 gctaatctgg ataaggttgc tgaagcggat ctagccgtaa tacgcaaaac gcttggcgtg
    10261 cagcgcctgc acgctgcgga actgggcttg caccgccttt ctgaccttgt agatacgctg
    10321 ctggttctgc agaaaaagca ccggatacgg tttgacatct ggcaggtagt aaagcgcgat
    10381 catgccatta tttcgttctt cgaccaggta tttgatcagg gaatgaatcc agccgtaccc
    10441 tggtctgcat actggacccc tctgcgctac cccctgctgc tgaatctggc cagcctgttt
    10501 gatgatgaac ttgcttcaaa cgcatggacg gcaaggctgg aggcgcatga tgagcgtgcg
    10561 tcggaacttt tttgtaccgt cagtgatgaa ctgatcagca ggacggcagc cagcgcgctg
    10621 gaccaccggt cgaagcagct gatcaccgat gccctgaact gggcgtcagc caattttgaa
    10681 cagttgggct ataactgtaa aaccaacaag gaaaggctgc ggattatgcc caatatgatt
    10741 ggctttcagt ccgtattgca cgggatctgt tcccgactgg gggcaccaga aagaaaggcc
    10801 agtatcattg ttgaccagca gtcacagttc aacaccaccc agcgtgaact taacgagttt
    10861 tactaccaga tacgggatat gccctgggaa ctggggcccg gattacctgt tatgaacatg
    10921 aaaaatatgc cggctgaacc acttgtattc cagtccggta ccaagagtgc aggtctggag
    10981 ttggtagata tttatctctg gacattcaag cgattcatgg aagacaaggc gctgacaaag
    11041 ccactttccc gccttgttta taccaatctg aaaacagcca ggaccaacag cgtgtcaatc
    11101 cagtcggttg ccagtcgatt taaggaactc ctgggtaaac tcccggtacc aagcgcagag
    11161 atcatgaggc aggcgcagga gctgcgggac tttgatgaag ccagacgcat gccatatgtg
    11221 gtgtcgggat cacccgactg atgccggata ttatctgctt tcaactgccc cctcttaggg
    11281 gcaggttttc aggttaaccg gcagctgtga tccacgacgc tgcttacagt catgctgcga
    11341 tgccagaatg tcagcaacct gataatcggt ccccatcgcc acatcgtgca atagcaggaa
    11401 aaagtccccg tcactgccgc ccctgcgcga atccagatac cgctgcagcc atcgggcatg
    11461 ataaagaacg tggcgctgtt cgcgctgcag ccatttcagc ggcagacggg acgtcatatc
    11521 gtcaaggata cgcatttcct gatgaagcgg cgcaataaca gcggaaaatt cctctctcag
    11581 tatccgggca ggtttcttct gtccgaacag cgcggccatt ttgctcagga catcctcgat
    11641 cgcccggctg actttctggg cctgatcacg ggaggcctga taacgcgtaa accaaaactt
    11701 acagtagaac cccgcagcgg caagcagcgc cgtaaggatc ggtccaatca gacttgtgtc
    11761 cacaatattc tccttaaaga tggtcatgag gccacctgaa gcagccgatg acgcaaatat
    11821 actccgtttt agcaattagt gctatgcgag aatgcaaaaa gtgctattcg ttgtttttgg
    11881 tgttgacatt ttaatttatg tgtgagtttt ttgttttctt tcagttttct ccactgagat
    11941 ccgaaaaccc caagttacgg atcttcctct ccctccgcac agcgttacat cccgtcagca
    12001 cagcatgtag tgcctcatac agttgcccat ggcactatat gttgtgttgt atctctggac
    12061 tgtgatgcgc cgcgcagggg cggaaaacag cgatatgatg attttctcag cgttgtacac
    12121 ttccggaaag tcgtttattc aaataaagtc ggatccatac gaaacgggaa tgcggtaatt
    12181 acgctttgtt tttataagtc agattttaat ttttattggt taacataacg aaaggtaaaa
    12241 tacataaggc ttactaaaag ccagatagca gtatgcgtat ttgcgcgctg atttttgcgg
    12301 cataagaata tatactgata tgtatacccg aagtatgtca aaaagaggtg tgctatgaag
    12361 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatgatgtc
    12421 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
    12481 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
    12541 ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa
    12601 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
    12661 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
    12721 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
    12781 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
    12841 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct ccgttataca
    12901 cagccagtct gcagtcatgg taccggcagt gtattctgcc gggcagcctg catcgctgcc
    12961 tgttgccatt gattatccgg cagctctggc acttcgccag atgtcgatgg tccatgatga
    13021 actgccaaaa tatctgctgg ctccggaagt gagtgccctg ctccattacg tcccggatct
    13081 gcaccgcaag atgctgctgg ccacactgtg gaacaccgga gcacgcatta atgaagcact
    13141 ggcgctgacg cggggggatt tttcgct
//
LOCUS       DAZGAN020000132        12936 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGAN020000132 DAZGAN020000000
VERSION     DAZGAN020000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.132"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..215
                     /locus_tag="ACHKYO_RS20935"
                     /old_locus_tag="pgaptmp_004187"
     CDS             <1..215
                     /locus_tag="ACHKYO_RS20935"
                     /old_locus_tag="pgaptmp_004187"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4430935.1"
                     /translation="LFVDPEYTRRGVASALLKPLIKSESELTVDASITAKPFFERYGF
                     QTVKQQRVECRGEWFINFYMRYKQQH"
     gene            461..1600
                     /locus_tag="ACHKYO_RS20940"
                     /old_locus_tag="pgaptmp_004188"
     CDS             461..1600
                     /locus_tag="ACHKYO_RS20940"
                     /old_locus_tag="pgaptmp_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HGU4430936.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            1597..2211
                     /gene="prfH"
                     /locus_tag="ACHKYO_RS20945"
                     /old_locus_tag="pgaptmp_004189"
     CDS             1597..2211
                     /gene="prfH"
                     /locus_tag="ACHKYO_RS20945"
                     /old_locus_tag="pgaptmp_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HGU4430937.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(2268..3725)
                     /gene="pepD"
                     /locus_tag="ACHKYO_RS20950"
                     /old_locus_tag="pgaptmp_004190"
     CDS             complement(2268..3725)
                     /gene="pepD"
                     /locus_tag="ACHKYO_RS20950"
                     /old_locus_tag="pgaptmp_004190"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU4430938.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3986..4444
                     /gene="gpt"
                     /locus_tag="ACHKYO_RS20955"
                     /old_locus_tag="pgaptmp_004191"
     CDS             3986..4444
                     /gene="gpt"
                     /locus_tag="ACHKYO_RS20955"
                     /old_locus_tag="pgaptmp_004191"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU4430939.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4536..5780
                     /gene="frsA"
                     /locus_tag="ACHKYO_RS20960"
                     /old_locus_tag="pgaptmp_004192"
     CDS             4536..5780
                     /gene="frsA"
                     /locus_tag="ACHKYO_RS20960"
                     /old_locus_tag="pgaptmp_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU4430940.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5838..6239
                     /gene="crl"
                     /locus_tag="ACHKYO_RS20965"
                     /old_locus_tag="pgaptmp_004193"
     CDS             5838..6239
                     /gene="crl"
                     /locus_tag="ACHKYO_RS20965"
                     /old_locus_tag="pgaptmp_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU4430941.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6278..7333)
                     /gene="phoE"
                     /locus_tag="ACHKYO_RS20970"
                     /old_locus_tag="pgaptmp_004194"
     CDS             complement(6278..7333)
                     /gene="phoE"
                     /locus_tag="ACHKYO_RS20970"
                     /old_locus_tag="pgaptmp_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGU4430942.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            7621..8724
                     /gene="proB"
                     /locus_tag="ACHKYO_RS20975"
                     /old_locus_tag="pgaptmp_004195"
     CDS             7621..8724
                     /gene="proB"
                     /locus_tag="ACHKYO_RS20975"
                     /old_locus_tag="pgaptmp_004195"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGU4430943.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8736..9989
                     /gene="proA"
                     /locus_tag="ACHKYO_RS20980"
                     /old_locus_tag="pgaptmp_004196"
     CDS             8736..9989
                     /gene="proA"
                     /locus_tag="ACHKYO_RS20980"
                     /old_locus_tag="pgaptmp_004196"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGU4430944.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            10104..10179
                     /locus_tag="ACHKYO_RS20985"
                     /old_locus_tag="pgaptmp_004197"
     tRNA            10104..10179
                     /locus_tag="ACHKYO_RS20985"
                     /old_locus_tag="pgaptmp_004197"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10137..10139,aa:Thr,seq:cgt)
     gene            complement(10306..10644)
                     /locus_tag="ACHKYO_RS20990"
                     /old_locus_tag="pgaptmp_004198"
                     /pseudo
     CDS             complement(10306..10644)
                     /locus_tag="ACHKYO_RS20990"
                     /old_locus_tag="pgaptmp_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(10695..11576)
                     /gene="paoD"
                     /locus_tag="ACHKYO_RS20995"
                     /old_locus_tag="pgaptmp_004199"
     CDS             complement(10695..11576)
                     /gene="paoD"
                     /locus_tag="ACHKYO_RS20995"
                     /old_locus_tag="pgaptmp_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HGU4430945.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIHGGVEAAAAFEALEMMGSGRDREIRYGEGSPWFDIVLPCGGGITLTLHKLRSA
                     QPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQTRTGWNLNGFEVGFRPCVRLMIYGR
                     SLEAQATASLAAATGYDSHIFDLFPASASAQIDTDTAVILLCHDLNRELPALQAAREA
                     KPFYLGALGSHRTHTLRLQKLHELGWSREETAQIRAPVGIFPKARDAHTLALSVLAEI
                     ASVRLHHEEDSCLPPSS"
     gene            complement(11586..>12936)
                     /gene="paoC"
                     /locus_tag="ACHKYO_RS21000"
                     /old_locus_tag="pgaptmp_004200"
     CDS             complement(11586..>12936)
                     /gene="paoC"
                     /locus_tag="ACHKYO_RS21000"
                     /old_locus_tag="pgaptmp_004200"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HGU4430946.1"
                     /translation="ATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLY
                     AGANRHTGLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDT
                     QVDPADPTRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNL
                     LEKSGARVHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFP
                     VSAGSGGQWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHE
                     ATAGGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRIL
                     NPKTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFL
                     DDTDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
BASE COUNT         3054 a         3313 c         3441 g         3128 t
ORIGIN      
        1 tgttatttgt tgaccctgaa tacacccgcc gtggggttgc cagcgctttg ttaaaacctt
       61 tgattaagtc tgaatccgaa cttacggtgg acgcaagcat aaccgcaaaa cccttttttg
      121 aacgttatgg ttttcagaca gttaagcagc aacgcgttga atgtcgggga gagtggttta
      181 ttaattttta tatgcgttat aaacagcaac attaaatcca gcttgcaatg aaaataacgc
      241 aagcctagta tgtgcgcgtt ttcttattcc acaaactgca aggaggtaaa tcatgacaaa
      301 tcctttatca atgactcttt gcagaccttt ccaggattaa ttcttttttt cttgccctgg
      361 attcgtctgc catttcctga ttttttaatt gttactctca ataattcaag tcgcaacaca
      421 tgcaacttga agtatgacga gtctatttac ggaatggtat atgggcaatt atattcgtcc
      481 cttatccgat gcggtattta ccatcgcatc tgatgacctg tggatcgaga gtttagcgat
      541 ccaacaatta cacaccacgg caaatttacc caacatgcag cgcgtagtgg ggatgccaga
      601 tttacatccc ggacgcggct atccgattgg cgcagcgttc ttctctgttg gtcgttttta
      661 tccggcactg gtcggcaatg atatcggctg cggtatggcg ctatggcaaa cagatattct
      721 cactcgcaaa tacaacgccg ataagtttga aaagcgatta tcagatctgg atgacgttgc
      781 tgaagaaagc tggctggagg aaaacctgcc gtcagcattt gcacagcatc cgtggcgcag
      841 ctcgcttggc tctatcggtg gcggcaatca tttcgctgaa ctgcaacaag ttgatcaaat
      901 catcaacgct gaactgtttg cactggcagg tctggatgca caacatctgc aactgttggt
      961 tcatagcggc tcgcgggggg tggggcagtc tattttacag cggcatattg cctcgttctc
     1021 gcatcatggt ttgcctcaag gtagtgacga cgcgctacgg tatattgcgg aacatgacga
     1081 tgcgctggcg tttgcgtgta ttaatcgcca gatgatcgct ttgcgcataa tgcaacaagt
     1141 taaggccacg ggaagtccgg ttctggatgt ggcgcataac tttgttagcg cgtgtcaaat
     1201 cggcgatcaa cagggctggt tgcatcgtaa aggtgccacg ccggatgaca acggtctggt
     1261 gattattccc ggttcacgtg gtgattactc ctggattgta cagcctgttg cgaacgaaaa
     1321 aacgctccac tcgctggcgc atggggcagg gcgtaaatgg gggcgcaccg agtgtaaagg
     1381 gcgtctggca gcgaaataca cggcgacgca actctcgcgt actgaacttg gcagtcgggt
     1441 aatttgtcgc gataaacaac tcatctttga agaagcgcca caagcctata aatcggctga
     1501 aagcgtggtg caatgtctgg tgcaggccgg tttaattatt cctgtcgcgc ggctgcgtcc
     1561 ggtgctaacg ctcaaaaaca gtggagggaa aaaaggatga tcttgctaca actctcctca
     1621 gcgcaggggc cggaagaatg ttgtctcgca gtgaaaaagg cgctggacag gctaattaaa
     1681 gaagctgccc gacaagacgt cgcggtaacg gtgctggaaa cagaaacggg ccactactct
     1741 gacacgctgc gttcggcgct gatttctctg gatggcgata acgcatgggc attaagcgaa
     1801 agctggtgtg gcactattca gtggatttgt ccgagtccgt atcggcctca tcatgggcgc
     1861 aaaaactggt ttctgggcat tgggcgtttt accgctgatg agcaggaaca atcggatgca
     1921 atccgttatg agacgttgcg ttcgtcgggg ccgggcggtc aacatgtcaa taaaaccgac
     1981 tcggcggtac gcgccacgca tttggcatcc ggtattagcg tgaaggttca gtcagagcgt
     2041 agtcagcatg ctaacaagcg gctggcacga ttgctgattg cctggaagct ggagcagcag
     2101 caacaggaaa atagcgcggt gctgaaatcg cagcggcgaa tgttccatca ccagattgaa
     2161 cgtggcaacc cgagacggac atttacaggg atggctttta tcgaaggata atgaaggatg
     2221 aaactgccgg aaggcgatta aacgccatcc ggcagcaaat caaataatta cttcgccgga
     2281 atttctttca gcagttcagt cagcagtgtc cagtaatgac ctacgctttc gatgtgaact
     2341 tgctcatccg gagagtgtgg accggtgata gttggcccga tagaaaccat gtccatttcc
     2401 ggatacggtt ttttgaacag accacattcc aggcccgcgt ggataatctg gatgttcggc
     2461 gttttgttga acagacgctg ataggtttca cgtaccagat gcatcaccgg agaattagcg
     2521 tccggctgcc agccaggata tgcgcctttc gcttcggttt tcgcgccagc cagtttaccc
     2581 agcgaatcca gcatgctcac cacgtagtct ttaccgctgt cgatcagtga acggatcagg
     2641 cagtgaattt ctacgttatt gtcagtcatg gtcaccacac cgacgttcag ggaggtttcg
     2701 accacacctt ttgccacgtc ggaattgcgg atcacaccgt tcggggtggc gttcagcaga
     2761 cgaataaagg tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc tacagagtcc
     2821 agcaacaagg ccagattttt ctctttctct gccagctcgt ttttcaggat ctcctgatag
     2881 gtattcacca gagatttcag gacgtcgact ttatcagctg cgacagcaat ggtcgcaaag
     2941 gcttcacgcg ggatggcgtt acgcagtgtg ccgccgttga aatcgataag gcgtagatcc
     3001 agttcttccg catgacccgc caggaagcgc accagtagtt tgttggcatt acccaggcca
     3061 acgtggattt caccgccgga gtgaccgcct ttcagacctt ttaaggttaa cttgaaggtt
     3121 tcaaaaccag ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa gtcgataccc
     3181 cccgcacaac ccatgtagat ttcaccttct tcttcggagt cggtgttaat cagaatatca
     3241 gcctgcaacc agttgctctg taagccgaac gcaccgtcca taccggcttc ttcggtcatg
     3301 gtcagcagca cttccagcgg gccgtgaacc acgttttcgt cagccagaac cgccagcgca
     3361 gacgccatac caatgccgtt atctgcaccc agcgtggtgc cgcgcgcttt aacccattcg
     3421 ccatcaatat aaggctggat aggatctttc gtgaagtcat gcacggtgtc gttatttttc
     3481 tgcggcacca tatcgaggtg ggcctgtaag acgaccggtt tacgattttc catacctgcg
     3541 gtagcaggtt tacgaatcag gatattacct acctgatcgc gttcgacatg gaaacctttc
     3601 tcttttgccc aaccaacaat gtattcagcg agttgctctt catgatagga cgggtgagga
     3661 atagaacaga ttttggcaaa aatatcccac agcggctgtg gagataattg agacagttca
     3721 gacacgttaa gtctccttgt cgatcacccg caaaacagta ttgcaggtca cagggttagc
     3781 agaaaatgtt gtcaacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
     3841 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
     3901 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
     3961 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
     4021 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
     4081 tattgccgta agccgtggcg gtctggtacc gggtgcgtta ctggcgcgtg aactgggtat
     4141 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
     4201 agtgctaaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
     4261 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcacatt ttgtgactat
     4321 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
     4381 tacctggatc gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
     4441 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
     4501 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
     4561 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
     4621 acggcgcaca accgcctgtg cagtcggctc ttgatggtaa aaccatccct cactggtatc
     4681 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgtgaa atcctcgacg
     4741 tccaggcacg tattgtgatg agcgatgctg aacgtaccga tgatgattta tacgataccg
     4801 tgattggcta ccgtggtggc aactggattt atgagtgggc cacccaggcg atggtgtggc
     4861 aacaaaaagc ctgtgcggaa gaagatccgc aactcagtgg tcgacactgg ctgcatgcgg
     4921 ctacgttgta caacattgcc gcctatcctc atctgaaagg agatgacctg gccgagcaag
     4981 cgcaggcttt atcaaaccgc gcctatgaag aggccgccca gcgtctgccg ggcacgatgc
     5041 ggcagatgga gtttaccgta cccggcggtg cgcccatcac cggctttttg catatgccga
     5101 aaggcgatgg cccgttcccg acagtattaa tgtgtggtgg tctggatgcg atgcagacgg
     5161 actattacag cctgtatgaa cgttattttg cgccgcgcgg cattgcgatg ctgactattg
     5221 atatgccgtc ggtgggcttt tcttcaaaat ggaagctcac ccaggactcc agcctgttgc
     5281 atcagcacgt cttaaaggcg ctgcctaacg taccgtgggt ggatcacact cgcgtcgcgg
     5341 cctttggttt ccgtttcggc gctaacgttg ccgtgcgtct ggcatacctt gaatcgccgc
     5401 gtctgaaagc ggttgcctgt cttggtccgg tagttcatac cctgttgagt gattttaagt
     5461 gccagcaaca ggtgccggaa atgtatcttg acgttctggc gagtcgtttg gggatgcatg
     5521 atgcttccga tgacgcgttg cgcgtggagc tgaatcgcta ttcattaaaa gtgcaaggat
     5581 tgctgggacg tcgctgccca acgccaatgt tatcaggcta ctggaagaac gatccgttca
     5641 gcccggaaga ggactcacgc ttaatcacct catcatctgc tgacggtaaa ttattagaga
     5701 tcccatttaa cccggtgtat cggaattttg acaaaggtct tcaggaaatc accggctgga
     5761 tcgaaaaacg cttgtgttaa aaatttgcta aattttgcca atttggtaaa acagttgcat
     5821 cacaacagga gatagcaatg acgttaccga gtggacaccc gaagagcaga ttgatcaaaa
     5881 aatttaccgc actaggcccg tatattcgtg aaggtaagtg cgaagataat cgattctttt
     5941 tcgattgtct ggctgtatgc gtcaacgtga aaccggcacc ggaagtgcgt gaattctggg
     6001 gctggtggat ggagctggaa gcgcaggaat cccgttttac ctacagctac cagtttggtc
     6061 tgttcgataa agcaggcgac tggaagagcg ttccggtaaa agacactgaa gtggttgaac
     6121 gactggagca caccctgcgt gagtttcacg agaagctgcg tgaactgctg acgacgctga
     6181 atctgaagct ggaaccggcg gatgattttc gtgacgagcc ggtgaagtta acggcgtgag
     6241 tgaaatgtgc cggatgcatc acatccggca ataatcatta aaactgatag gtcatgccaa
     6301 ccgcgacaat atcatcatta ttaatattca atttgttatc gctatccagt tggttgattt
     6361 tataatcaac aaacgctgac atatttttgt tgaaataata tgtagccccg acatcgatat
     6421 aattgaccag atcttcatca ccgatacctt caatatcttt ccctttcgat aagacataac
     6481 ccagcgatgg acgcagacca aagtcaaact ggtattgagc gaccgcttca aagttctgtg
     6541 tcttattggc aaagccgcca gttattggcg tcattttgcg tgtttcagaa taaaaagttg
     6601 ccagataaat attattggca tcgtatttca gacctgttgc ccatgcttct gcacgcttgc
     6661 cagtgccacg gctttgcagg ttctgctcgt tggtgcgatc tgagttggta taggcaccac
     6721 taatggcgaa atcgctgcca ccaaagtcat atgtcaatga cgtgccgaag ccatcgccgt
     6781 tttgcttttt aacgtcgcgg ttttcgtttt tcccttgata ttgcagggtt aagttcaggc
     6841 catcgataac gccgaagaag tcggtgttcc gatacgtcgc cagaccgctg gcgcgtttgg
     6901 tcataaagtt gtcggtctgc gccgaggagt cgccaccaaa ttccgggaac atatcggtcc
     6961 aggcttccac gtcatacaac gcgcccaggt tacggccata gtcgaaagaa cccaaatctt
     7021 tatacttcaa tccggcaaaa gcgagacgcg ttttttgctg tgcagtatca ctctccgctt
     7081 tatttccggc aaactccgct tcccaacggc catagccagt cagttgatcg ttaatttgtg
     7141 tttcgccttt aaaaccaaaa cggatataac tctggtcgcc atctttactg tcgttatcac
     7201 tcatataatg catggcttta actttgccat agacatccag tttattaccg tctttgttat
     7261 atatttctgc ggcctgtacg gatgcagatg ccacaatgcc catcaccact aatgccagag
     7321 tgctcttttt cattttcatt cctgatttta attaacgcgc gaatattcag cgggagagcc
     7381 ccgttgaaaa caggaaagtt tttaacctga gattgttaaa gatatattac agattaataa
     7441 tattcttaaa atgtggtaat ttattaaatc tgtaataaaa gcgtaaacaa ctgccgctag
     7501 gcttgctgat cccgcgcaac aaaacgccat gctttgcttg cagatggttg gcaaccgacg
     7561 acagtcctgc taaaacgttc gtttgatatc atttttccta aaattgaatg gcagagaatc
     7621 atgagtgaca gccagacgct ggtggtaaaa ctcggcacca gtgtgctaac aggcggatcg
     7681 cgccgtctga accgtgccca tatcgttgaa cttgttcgcc agtgcgcgca gttacatgcc
     7741 gccgggcatc ggattgttat tgtgacgtcg ggcgcgatcg ccgccggacg tgagcacctg
     7801 gggtacccgg aactgccagc gactatcgcc tcgaaacaac tgctggcggc ggtagggcag
     7861 agtcgactga ttcaactgtg ggaacagctg ttttcgattt atggcattca cgtcgggcag
     7921 atgctgctga cccgtgctga tatggaagac cgtgaacgct tcctgaacgc ccgcgacacc
     7981 ctgcgtgcgt tgctcgataa caatatcgtt ccggtaatca atgagaacga tgctgtcgct
     8041 acggcagaga ttaaggtcgg cgataacgat aacctttctg cgctggcggc gattctggcg
     8101 ggtgccgata aactgttgct gctgaccgat caaaaaggtt tgtataccgc tgatccgcgc
     8161 agcaatccgc aggcagaact gattaaagat gtttacggca ttgatgacgc actgcgcgcg
     8221 attgccggtg acagcgtttc aggcctcgga actggcggca tgagtaccaa attgcaggcc
     8281 gctgacgtgg cttgccgtgc gggtatcgac accattattg ccgcgggcag caagccgggc
     8341 gttattggtg atgtgatgga aggcatttcc gtcggtacgc tgttccatgc ccaggcgact
     8401 ccgcttgaaa accgtaaacg ctggattttc ggtgcgccgc cggcgggtga aatcacggta
     8461 gatgaagggg caactgccgc cattcttgaa cgcggcagct ccctgttgcc gaaaggcatt
     8521 aaaagcgtga ctggcaattt ctcgcgtggt gaagtcatcc gcatttgcaa cctcgaaggt
     8581 cgcgatatcg cccacggcgt cagtcgttac aacagcgatg cattacgccg tattgccggg
     8641 caccactcgc aagaaattga tgcaatactg ggatatgaat acggcccggt tgccgttcac
     8701 cgtgatgaca tgattacccg ttaaggagca ggctgatgct ggaacaaatg ggcattgccg
     8761 cgaagcaagc ctcgtataaa ttagcgcaac tctccagccg cgaaaaaaat cgcgtgctgg
     8821 aaaaaatcgc cgatgaactg gaagcacaaa gcgaaatcat cctcaacgct aacgcccagg
     8881 atgttgctga cgcgcgtgcc aatggccttg gcgaagcgat gcttgaccgt ctggcactga
     8941 cgcccgcacg gctgaaaggc attgccgatg atgtgcgcca ggtgtgtaac ctcgccgatc
     9001 cggtggggca ggtaatcgat ggcagcgtac tggacagcgg cctgcgtctt gagcgtcgtc
     9061 gcgtgccgct gggggtgatt ggcgtgattt atgaagcgcg cccgaacgtg acggttgatg
     9121 tcgcttcgct gtgcctgaaa accggtaatg cggtgatcct gcgtggtggc aaagaaacct
     9181 gtcgcactaa cgcggcaacg gtggcggtga ttcaggacgc cctgaaatcc tgtggcttac
     9241 cggcgggtgc cgtgcaggcg attgataatc ctgaccgtgc gctggtcagt gaaatgctgc
     9301 gtatggataa atacatcgac atgctgatcc cgcgcggcgg ggctggtttg cataaactgt
     9361 gccgcgaaca gtcgacaatc ccggtgatca caggtggtat aggcgtatgc catatttacg
     9421 ttgatgaaag cgcagagatc gctgaagcat taaaagtgat cgtcaacgcg aaaactcagc
     9481 gaccgagcac atgtaatacg gtagaaacgt tgctggtgaa taaaaacatc gccgatagct
     9541 tcctgcccgc attaagcaaa caaatggcgg aaagcggcgt gacattacac gcagatgcag
     9601 ctgcgctggc gcagttgcag acaggccctg cgaaggtggt ggcggttaaa gccgaagagt
     9661 atgacgatga atttctgtca ttagatttga acgttaaaat cgtcagcgat cttgacgatg
     9721 ccatcgccca tattcgtgaa cacggcacac aacactccga tgcgatcctg acccgcgata
     9781 tgcgcaacgc ccagcgtttt gttaacgaag tggattcgtc cgctgtttac gttaacgcct
     9841 ctacgcgttt taccgacggc ggccagtttg gtctgggtgc ggaagtggcg gtaagcacac
     9901 aaaaactcca cgcgcgtggc ccaatggggc tggaagcact gaccacttac aagtggatcg
     9961 gcattggtga ttacaccatt cgtgcgtaaa taaaaccggg tgatgcaaaa gtagccattt
    10021 gattcacaag gccattgacg catcgcccgg ttagttttaa ccttgtccac cgtgattcac
    10081 gttcgtgaac atgtcctttc agggccgata tagctcagtt ggtagagcag cgcattcgta
    10141 atgcgaaggt cgtaggttcg actcctatta tcggcaccat taaaatcaaa gagttacccc
    10201 atatttaaat acaccacgtt tcctcctgtg ccgcatttgt gccattgtaa ccttggcaat
    10261 tcatcaaaat actgttctga catcaggcag tgcaggtgca gctatttaaa ccaattgctg
    10321 ccgccattct ttgacgtagt caatcagggc gcggagcttt ggtgcaatat tgcgacgctg
    10381 tgggaaatac agatagaagc ccggaaattg tggaagaaag tcatcaagca gcgatacaag
    10441 cttaccgctt tcaatatatg gcctgaaagt ttcctgagtg gcaattgtta ttcctccgcc
    10501 ggcaagagcc agcctcaaca tcagacgcag atcattagtc gtaatctgcg gttcaatcgc
    10561 aaggtcgaaa gttctcccgt tttcttcaaa tggccagcga taaggcgcaa cctccgggga
    10621 ctgacgccag ccgatacact tatgggtatt tcccccggag gcgagaaagc gctctccacg
    10681 cccggccgca aggatcagga cgacgggggc aggcatgaat cctcctcatg atggagacgt
    10741 acagaggcga tttctgccag cacggagagt gccagagtat gcgcatcccg ggctttgggg
    10801 aatatcccga cgggggcccg gatttgcgct gtctcctccc tggaccatcc cagctcgtgg
    10861 agcttttgca gacgtaacgt gtgggttcga tggctgccca atgcgccgag ataaaagggt
    10921 tttgcttctc gcgcggcctg caatgctggc agctcccggt tgagatcatg gcacagcaaa
    10981 atgaccgccg tgtcggtatc gatctgagcg ctggctgagg ccggaaaaag atcgaagata
    11041 tggctgtcat agcctgtggc tgctgcaaga ctcgcggttg cctgcgcctc aagagaacgt
    11101 ccgtaaatca tcagcctgac gcatggcctg aaccccacct caaagccatt gagattccag
    11161 cccgttcggg tttgcgtggg caggcacacc aacgattgtg cttgcggatc gtagcgcagc
    11221 cccgccggtt ttctctgttc cagacggttc agcacggcga gcagaggctg tgccgagcgt
    11281 agtttatgga gcgtaagcgt gatcccaccg ccgcagggca gaacgatgtc aaaccacggc
    11341 gaaccttcgc catagcgaat ttcgcgatcg cggcctgagc ccatcatctc cagcgcttca
    11401 aaagcggcag cggcctccac gccgccgtgt atctccacca gcgtcaccag caccgctcct
    11461 ttaccggaca ttagcgcctc tacggcgaag cgcagaatgg tccggtggtc atcggtgaga
    11521 aacgcctgct ctggcttatg ccagtgttcg tctttatcaa aaagcgggta tgacattgtt
    11581 tctccttaaa ccacatccgg cagcttatcg agcagcttat ccagagtgat gggataatcc
    11641 cgtacccgaa taccggtggc gttatacacc gcgttggcga tagccgcgct cacgccgcac
    11701 aggcccaact caccgacacc tttggccttc atcggggagg atatggggtc ggtatcatcc
    11761 aggaaaatca cctcctgttt tgggatatcc gcatggaccg gcacctcata ccccgccata
    11821 tcgtgattaa cgaagtagcc caaacggtca tccaccgcca gctcctccat cagtgccgcg
    11881 cccatgccca tagtcattgc gccaatgacc tggctgcgcg cagttttcgg attcaggatg
    11941 cgtcctgcag cacacacagc gagcatacgc cggacccgaa cttctcccgt cgcgctatgc
    12001 acgccgacct ccacaaaatg cccggcaaag gtcgactgct ggtactcttt gctcagtgtt
    12061 ccgaattcaa tgctctcttc cgctgtcagt ctgccgcctg cggtggcttc atgtagcgtg
    12121 gcgcttcggg taccgttggt aatcttgccg tcggcaaact gcgactgctc aggatcaaac
    12181 ccgactgccg aggcaatcat ttcgcgaagc ttcatacagg cggcgtaaac gccggaggtg
    12241 gaggtattcg cgccccattg tccaccagaa cccgcagaaa ccgggaaact ggaatcgccg
    12301 aggtgaaccg caacctgctc cagcggtacg ccaagcattt ccgctgccgt ctgggccaga
    12361 atggtgtagc tgccggtgcc aatgtcggtc atgtccgttt ctacggtaac ggtgccgttt
    12421 tgttcgaggt gaacccgagc acccgatttt tccagcagat tattgcgaaa gcccgccgca
    12481 acaccgtggc cgactagcca ctccccgtcg cgcacctgtc cgggtgtggc gttgcgctgc
    12541 ttccagccaa atttatccgc tccggtgcgc aagcactcga taagctgacg gcgagagaag
    12601 cagcgcgtcg ggtcggcggg gtcaacctga gtgtcattca ggatgcgaaa ctcgacggga
    12661 tcgatgcccg ctttttccgc cagttcgtcg atcgcgattt cgagcgccat cagaccgggg
    12721 gcttcgccgg gcgcacgcat ggcgttccct tccggcaaat caagcgtggc gagccgcagg
    12781 ccggtatgac gattcgcccc ggcgtagagt aattcgcttt gctgtaccgc cgtttccggc
    12841 gtgccgccgg gcaggtttcc agaccagctt tcatgtgaga tagcggtgat tttcccgctc
    12901 tggtcggcac cgatacgcaa gtgctgaagg gtggcg
//
LOCUS       DAZGAN020000133        12696 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGAN020000133 DAZGAN020000000
VERSION     DAZGAN020000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.133"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..887
                     /locus_tag="ACHKYO_RS21005"
                     /old_locus_tag="pgaptmp_004201"
     CDS             <1..887
                     /locus_tag="ACHKYO_RS21005"
                     /old_locus_tag="pgaptmp_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4430947.1"
                     /translation="MFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKE
                     QLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLP
                     FDSIDIISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALL
                     NKFAVFSSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIM
                     LASRKAHELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKK
                     CRWPIRNVPWGASSVKYS"
     gene            complement(1004..2347)
                     /gene="gdhA"
                     /locus_tag="ACHKYO_RS21010"
                     /old_locus_tag="pgaptmp_004202"
     CDS             complement(1004..2347)
                     /gene="gdhA"
                     /locus_tag="ACHKYO_RS21010"
                     /old_locus_tag="pgaptmp_004202"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGU4430948.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            2583..2855
                     /locus_tag="ACHKYO_RS21015"
                     /old_locus_tag="pgaptmp_004203"
     CDS             2583..2855
                     /locus_tag="ACHKYO_RS21015"
                     /old_locus_tag="pgaptmp_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HGU4430949.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(2821..3228)
                     /gene="nudG"
                     /locus_tag="ACHKYO_RS21020"
                     /old_locus_tag="pgaptmp_004204"
     CDS             complement(2821..3228)
                     /gene="nudG"
                     /locus_tag="ACHKYO_RS21020"
                     /old_locus_tag="pgaptmp_004204"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HGU4430950.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            3315..3935
                     /locus_tag="ACHKYO_RS21025"
                     /old_locus_tag="pgaptmp_004205"
     CDS             3315..3935
                     /locus_tag="ACHKYO_RS21025"
                     /old_locus_tag="pgaptmp_004205"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4430951.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            complement(3944..5251)
                     /gene="ynjE"
                     /locus_tag="ACHKYO_RS21030"
                     /old_locus_tag="pgaptmp_004206"
                     /pseudo
     CDS             complement(3944..5251)
                     /gene="ynjE"
                     /locus_tag="ACHKYO_RS21030"
                     /old_locus_tag="pgaptmp_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(5318..5971)
                     /locus_tag="ACHKYO_RS21035"
                     /old_locus_tag="pgaptmp_004207"
     CDS             complement(5318..5971)
                     /locus_tag="ACHKYO_RS21035"
                     /old_locus_tag="pgaptmp_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU4430952.1"
                     /translation="MLCVKNVSLHLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(5971..7506)
                     /locus_tag="ACHKYO_RS21040"
                     /old_locus_tag="pgaptmp_004208"
     CDS             complement(5971..7506)
                     /locus_tag="ACHKYO_RS21040"
                     /old_locus_tag="pgaptmp_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGU4430953.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFAEGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(7479..8645)
                     /locus_tag="ACHKYO_RS21045"
                     /old_locus_tag="pgaptmp_004209"
     CDS             complement(7479..8645)
                     /locus_tag="ACHKYO_RS21045"
                     /old_locus_tag="pgaptmp_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4430954.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(8655..9203)
                     /locus_tag="ACHKYO_RS21050"
                     /old_locus_tag="pgaptmp_004210"
     CDS             complement(8655..9203)
                     /locus_tag="ACHKYO_RS21050"
                     /old_locus_tag="pgaptmp_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGU4430955.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(9203..9910)
                     /locus_tag="ACHKYO_RS21055"
                     /old_locus_tag="pgaptmp_004211"
     CDS             complement(9203..9910)
                     /locus_tag="ACHKYO_RS21055"
                     /old_locus_tag="pgaptmp_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU4430956.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(9925..10602)
                     /gene="ydjY"
                     /locus_tag="ACHKYO_RS21060"
                     /old_locus_tag="pgaptmp_004212"
     CDS             complement(9925..10602)
                     /gene="ydjY"
                     /locus_tag="ACHKYO_RS21060"
                     /old_locus_tag="pgaptmp_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HGU4430957.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(10607..11317)
                     /locus_tag="ACHKYO_RS21065"
                     /old_locus_tag="pgaptmp_004213"
     CDS             complement(10607..11317)
                     /locus_tag="ACHKYO_RS21065"
                     /old_locus_tag="pgaptmp_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU4430958.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(11484..12290)
                     /gene="xthA"
                     /locus_tag="ACHKYO_RS21070"
                     /old_locus_tag="pgaptmp_004214"
     CDS             complement(11484..12290)
                     /gene="xthA"
                     /locus_tag="ACHKYO_RS21070"
                     /old_locus_tag="pgaptmp_004214"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGU4430959.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         3109 a         3309 c         3251 g         3027 t
ORIGIN      
        1 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattactg
       61 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
      121 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
      181 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
      241 aaacgctgga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
      301 tgcttccttt tgactcgatc gatatcataa gtaaaagacg cgagtcttta gaggatcaat
      361 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
      421 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
      481 tgaataaatt tgctgttttt tcctcagact atatctctga ttgcgctaat aatcgctccg
      541 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
      601 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
      661 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
      721 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
      781 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
      841 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
      901 tgccgggggc gctgcgcttg cccggcctac attagagcgt aactgcaatg aattgtgccc
      961 atttgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
     1021 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
     1081 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
     1141 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgcgccattt
     1201 caaggcctga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
     1261 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatttgcc ccttcggcga
     1321 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
     1381 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
     1441 ccagaccaaa ttctttggcg taatctgcca ctcgaccatc gcggctggct ttgatttcga
     1501 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
     1561 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
     1621 cgttgccgga gccagaaacg gaaacgcgca tcccttcaaa acccataccg tggcgtttta
     1681 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
     1741 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
     1801 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
     1861 cggtatccgc gcccagatga cgataaagct cggtcatcag cgcctggcag aaacgcatca
     1921 cttcgccttc gcttttacct ttcgggtcaa aatcgctacc gcctttacca ccgcccatcg
     1981 gcagggtagt cagggcattt ttgaaggttt gttcgaagcc gaggaatttg agaatggaga
     2041 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggct gagctgaact
     2101 gcacacgcca tgcacggttg acctgtacct ggttgcgatc atcaacccat accacgcgaa
     2161 actggatcac gcgctccggt tcaaccagac gctccagtaa tgacatctgg cgatattttg
     2221 gattttgttc aagaaaaggc cagagtgtgg tcattacttc acgaacggct tgcgcgaact
     2281 cggtttgatt cgggtcgcgc ttttggacat ggttgaggaa tgactccaga gaatatgtct
     2341 gatccataga tataaaaccc ttatatatta atacgattgt gcttatgttg tttatgtgat
     2401 gtgcttttgc agttttcgac tataccatca agaatgtgac ggtaagcaag aaaaaaatga
     2461 cccaggaaag caaattaata acgagagtaa tctcataacc aaatgcgata actcagacgc
     2521 taaagttttg ccacccggct accgttatca gtagcagaga caataacgtt aaggagttcc
     2581 ctgtgagtcg agcattgttc gccgtagtat tagcatttcc attaattgca ctggctaacc
     2641 cgcattatcg tccggacgtg gaggtaaacg taccgccaga agtgttcagt tcaggcgggc
     2701 agtcagcgca gccctgtact cagtgctgcg tttatcagga taaaaattat tctgaaggcg
     2761 cggtaattaa agccgaggga atactgctgc aatgccagcg ggatgacaaa acccttagca
     2821 ctaatccgct ggtctggcgg cgcgtaaagc cataaacgcc tctaataatg gaatgtcagc
     2881 aggggccagc ggatattgca gcgcctcttc aggtgagcac cagaccagcg cctgatgttc
     2941 atgtgcctgt aacgtcccgt ggaagtcggg tacgtgccag gcatgaagat ggataatccg
     3001 gcccgaaact tctcgctgat ggctggtaac atactcaccc acagttgctt cgatgcccag
     3061 ttcttcgcgt aactcacgca ccagcgcctg ccgctggctt tcatccggct cgactttacc
     3121 accggcaaac tcccataatc ccgcctgatc gctctgggcg gggcgttgcg cgagtaaaat
     3181 tttgccatca cgttcaatga tggcggcaac aacttcaatc attttcataa attacacacg
     3241 ggcagataag cgaaaaatgc cataatatcc gcactgttgc tcaatcagaa aggctttata
     3301 tttaaggagt gcctgtgcta gaccgccatc ttcatccccg gattaaaccg ttgttgcatc
     3361 agtgcgtgcg ggttcttgat aaaccgggca ttacgccaga tggtttaaca ttagtgggat
     3421 ttgccatcgg cgtgctggca ctgccttttc tggcgctggg ctggtatctg gcggcgcttg
     3481 tcgtcatttt gttgaacagg ctgcttgatg gtctggacgg tgcgctggcg cggcgcagag
     3541 ggcttaccga cgcgggcggc tttcttgata tttctctcga ttttcttttt tacgcgctgg
     3601 taccttttgg gtttatcctc gccgcaccgc agcaaaacgc cttggcaggc ggctggctgc
     3661 tgtttgcgtt tatcggcaca ggcagcagtt ttctcgcttt tgctgcgctg gcggcgaaac
     3721 atcagattga taatcccggt tatgcgcata aatcgtttta ttatctgggc gggttaaccg
     3781 aaggcacaga aacgatctta ctgtttgtgc tgggatgttt attccctgca tggtttgcgt
     3841 ggtttgcgtg gatcttcggt gcgttgtgct ggatgacgac ctttacgcgg gtgtggagtg
     3901 ggtatctgac gctgaagtcg caccagcgtc agtaattgca atgttatttg ctactgtccg
     3961 ggccgcgttc accggttgct accggatttt ttggatcgct gctccattcg taccagccgc
     4021 cgtcatacac ggaaacattc ttccaaccca tggcgcgtgc gtacataaag gtttcggacg
     4081 cgcgccagcc ggtgccgcag tagaatgaaa cttgctgctc tggtttgata ttccacgctt
     4141 tccacatagc ggtaatatca tcggcgctgc gcatggtgcc atccgggtta tgaaaatctt
     4201 ccatatgcgt cgagtcgcta ccagcgtgtc cccaacgtgc tccggctatt tcaccttttg
     4261 gtttaatata gctgtaaccg ctggtcgtac cgataaattc tggccacgaa cgaatgctca
     4321 ccagcgatgc atcctggcga tgcagcagtc cacgggcctg ttccatatcg agcatcaact
     4381 gcggttgtgc cgggatcttc acgccgaaat ccggttccgc tttcactttc ggtggcgttc
     4441 ctcgctcaac aggcagtccc gcgtcggacc aggtttgcca gccgccatcc agcagacgca
     4501 catctttcac gcctgcgtag agcataattt gcgccacacg cgctccagcg tatacgtcac
     4561 gcccgtacag aatgaccgtg gtgtcatggc gaatgccgtg ttttgccagc atcgctttta
     4621 gttgttcatc agaaactttg ttccacagcg gttcgctttc cacttcgttg gtatcgatgt
     4681 agtcagcacc gggaatatgg ctgataaggt aaagcttagg agcgccccag gccgcttcaa
     4741 tgactttcca gtcaccggca ggtttcgccg taacctcttt accttgttgc aggtcgtgca
     4801 gccattgcgg ataaaccagc tgctcaaagt gcggcagttt ttgtagacgg gaaggttcgc
     4861 ttagcgcgtc actcagaatg gagatatgcg taaaacctgc tttttgcagt cgcgttttga
     4921 cggcgtcgac atctttgtca ttaccgtaca gcgccaccgg ggcatcggct ttcaggttat
     4981 gttgcttgat ccacgcgttg agctgttcgg tgctcatttt gtcgagccag ctggcagaga
     5041 ggttttaggc ggcaggttca tgaccagaag ggccatttaa ggtttgtggc cagccgttat
     5101 aaaacgcgct ggggcgagta tctatcgctt tgccattttg ttgttgaagc tggtcaggtg
     5161 taagaggctt cgccagttca gcggcccacg aagaagcgca agccagccct aaagccattg
     5221 ccagcgcggt catttgagaa acacgtttca tcgaaaatcc cgacattgta gttttgagtg
     5281 cgccattctg aactgacgca ttgagaaaaa ctttgcgtta tcgcagtttg ttgtaatttt
     5341 ctgaccactg cgccatatcc agaacagaac tatcagcagg aacatcctgg agatcgtgcg
     5401 ttacctgaac gacggggatc gccagttcgc gaacttcgct gaacacccac tggcgaaaat
     5461 tatcgcgcag agccacatca agacggctga atggctcatc caggagtaac gcttttggct
     5521 gagcgagaag ggcgcgcagc agggccaccc gcgctcgctg accgcctgat aatgtcgcag
     5581 ggtcctggtg ataagtttca gccagtcccg ctcgatcaag cgcgtctttc acggcattgc
     5641 gcctggcagt ccccttgagc gtcgacggta gcgccagcag taaattttgc ccgacgctga
     5701 actggtcaaa taacagtgca tcctggaaaa gaatgccaat ctgacgctgt gcggtgggta
     5761 gcatgtcaat ccgttgctca ttgagccata gctcacctgt acaagaaaac tgtccagcca
     5821 gtgcaccaat catccatgaa aacagagtgg attttccaca gccagacggc cccattaacg
     5881 tgacaatgtc acctttatcc accgtaaagt taacgtttgt cagcaagcgg ctttctggta
     5941 aatgtagcga aacatttttc acgcagagca ttagcggagt ccttgtctga cataacctac
     6001 ccattttgcg actaacgcgg tcagggcaaa aataataagt ggtaatagca gttgccataa
     6061 agcctgggcg gcgaaaatac cattgctgcc gccgctgctt aatgccaccg cctcagtggt
     6121 gagcgtcgga aaacgccccg cgcccagcca cagcgtcggc atatactgcg caatactgac
     6181 tgaaaatccc accgcgaagg caatcagcgt agggcgtagc ataagcggac atttcacgta
     6241 aaagaaaatt ttggcccgcg accagctcag tgtttgcgca atcaaaatta accgtgaatc
     6301 aatgcgctgc caggcgggtt gcaggataaa cagcatccac ggcattaccc acagcagatg
     6361 cccccagacc accgccgtcc agcttccatc caggttcagc catagcgcca gcgtgtactg
     6421 gcccgccacc agtggcagag cgggcaatag aatgggcaac cagagccaca actggcgacg
     6481 ctgtggcccc cattccagcc acagcagtaa caggagcaaa gcgatgaatg tcgccaccag
     6541 ccccattgtc aggctgttga tgagtgcttc actattgatc gtcgactgat ccgcgaggat
     6601 cgccagcaga accacacaca gcacaccggt taagggtaaa aaactcgcca gcgtagtgcc
     6661 tggtaataaa ggtgtggcag gcttgcgaat gccatctacg cggggaatag tgcgccgcca
     6721 gctgcgccat agcagatagc tcagcaaaac gtaggcggcg agtaacagca tcagcagcag
     6781 actggcgagt gcgcctttcg tttgttgatc cgcgtcgccc tgagttaacc actgccagct
     6841 aattactgcc agcgtcggtg gattacctgg cccaagaata atcgccacat ccacgaccga
     6901 aagcgaccag gcaacaatcg ccaacatcgc cattgccagt gcaggtgcga cggagggcaa
     6961 gagcagccag ttcaggcatt gccagcggct gtagcccagc gaatccagca caatgacctg
     7021 ctgcaacagc cgtttttcgc tcaatactgc cgctaagatc cacagcagaa atgcgctttc
     7081 tttcactgca agggtgaggc ccagcccaat acccaatttg tccattagcg gagtgaaata
     7141 cgggaaatag tcataaagca gccctccctc agcaaagagc agaagggcgc tggtggcaaa
     7201 agccacatgg ggaatggcga gcagccacgg cagacgggca cacatacgct gccatttcgg
     7261 ccaaggccac agcgccacaa tcaccagcag ggcaatcaac aacgccccga ccgccgcgat
     7321 ggttgtcgac accagcgttg ccagtaatgc ctgcggtaac tgtggatcgg caaataacgc
     7381 ctgccagtgt gtcaacgaca aagcaggcga tatcagcgtg agagctgccg ggatcagtgg
     7441 tgcataaatc accgccacca tcgcccacag caaaaagatt aatgcgtacc gtaacggtgt
     7501 agccattctt gttccagcgc atttacccaa cctgcgtgcg gttcagccag taccggcggc
     7561 agatcttgcg gcattcttga ttgcaatgtt tcgcgctgcc catcaggcag tttttgcgga
     7621 tcgagaacag aaggatcgcc ccagacagcg ggatccgctt tacgcagttg cgcattgggt
     7681 gagagcagga agttggcaac caccttcgcc gcagcactgg catttgcgtt agcaggaatg
     7741 gtgacgaaat gcacgttgcc aatcatcccc tggttaaaac caaaactgta actgcttgca
     7801 ggcaaatcgc cgctggcgat tttttgctgg gcatgagcag ggttaaaggt cagcgacagg
     7861 cgcaatgtgc cggctttcag cagagcatcc atccgcgcgg gcgaaggcgg gaaatccttt
     7921 cctttgcgcc ataaataggg atgtagcgcg tcaagatatt gccacaaggg cgcagtgacg
     7981 cgggcgaaag tggctttatc cggggcaact tttaacgcgg cgggatcggg cgtcagcata
     8041 atcagcaact gttcaagaaa cgccgtgccg gtaaagtccg gtgggcgcgg ataggtaacc
     8101 gtgccgggat tggctttagc aaactccagt aaggcttgcg gcgtttgtgg tggctgtggc
     8161 gtaacatcgc ggcgggcgat aaacgtcagt tgtgcgccgc cccagggcga ttcagcccct
     8221 tctgtaggca ctgaaaaatc ttcccgcacc ggcagctgtg tgtcgacata gcgccagttg
     8281 ggcagagtct ccgcccagcc cgtttgtaat aaattggcct cttttaaggt gcggaagttt
     8341 tcgccgttca cccagagcag atccaccgag ccgcccgttt tacgtccggc agcagcttcg
     8401 gtctgaatgc gcttcaccgc gtctgcggca tccgccagac gaacaatctt caggtttata
     8461 gcgtaatggg ttttcatctc gccgctaacc cagtcgagat agcggttaat tgcggtatcg
     8521 ccgccccagg cgttaaacca gacggtttgc cctttggcct catttttaat ttcctgccag
     8581 tcactggcat gtgttgccag agaaaacagc gataacaatc ccagcaacca cccacaatgg
     8641 cgcatcttct ctccttaaga acgtttttcc ggaatacggc acagcccctg agcgggaatg
     8701 tccatggcag aattaaaacg agccgacagg ttttgcaggc cgatcaatgc cgtcagttcg
     8761 gtgagcgcct gagcgtcaaa atgcgcagcc agtcgggtgc gcagggcatc atcgaccgtt
     8821 ggcggcgtta cgcttgcggc ttcggcgtac tccagcgcca gccgttcttc atcgctgaag
     8881 agcgggcttt gccgccagtc agccactgcc agcagtttat cggtgctgcc ggtacgctcg
     8941 gcgactttca tactggtgat atccacgcaa aactcacaca ggcacatttg cgcaatgcgc
     9001 gcgctgacaa gcgatcgcac taccggatcg agcggtgagc gtttgcgctc cagccaccca
     9061 acaaacattg acaccaaata aaagataaac gggatccgcc cccaccagcg aatcgggctt
     9121 agcacctcgc cgtaatggcg acgatgcagc cacgcctgcg gacgtaaaat gaaaggaatt
     9181 ttgctaagcg gcggtaaacc catcaggcat tcctcttctg gcgctcaagc catattttct
     9241 tcgccataaa aatcaccacg gtcagggcaa acagaataaa cagtccggtg acaaaccaga
     9301 aagtaccgcc tgttaacatg ctgcccgccc aggaataaac aatagtcgcc ggtaattgac
     9361 caagcccggt ggcgataaaa aacgagcgaa aacgtattga agtcagaccg gcagcatagc
     9421 tgattggatc gaaagggaca aaaggcaata accgacagac caggatggtg tgtttgccgt
     9481 agcgagtgaa aaagccgtcc atactgtcaa gcacggtttt gccggttaat ttttccacca
     9541 cttcgcggcc catcactctg gcgataaaaa agcacagcgc cgcgccggcc atcgaactgg
     9601 tccacgacag caagccgccc cagaacgcgc caaacagcga cgcattggca aaggtgatca
     9661 aaaacgcagg tagcggtgca gcaatggcct gtaaaatcat caacaagaac gagacaactg
     9721 ccgccagtgc gccgtaagac tgaataaagc gttctatacc ctgttggtcc acggcggcaa
     9781 acgccgcaac gctgcgattg ataaactcat ggacgccggg aagcagcgcc catgcgagca
     9841 gcatagcgaa caacaggatg atgagggtta tacggtagta ccagatttta cgcgattgta
     9901 tcttcatcat cctttatcct ggctttattc ggtgatttta aaggtaaccg ttgccagcgt
     9961 gttatccgcc gggagaactg aggcattgcc tttgaatttc acttcgccgc gtttttccac
    10021 cgcaccataa gtgtatgttg cattgctgac gatgccgacc gggcagctat ccagacacac
    10081 caggcaaccc gttttcttct cttctgctgc cgttaaatta ccgccaaagc gcatgtccag
    10141 tttcttgcca ttactgtcaa caatcacttc atcgaaggaa tacgcttttg ccgccccttg
    10201 ccagttgacc gaaatatcca gtttgctgcc tgtgacatga gtcgtttctt tattatccat
    10261 cgtcatgttt tcgcccggcg taccacctgc ctctttcagg gcttcataaa atgctttcgg
    10321 ggtcgcataa gccataaaca gcgatttatg tccgttggag ccatctttaa acacaatacc
    10381 gtgacgagtg tcgtcggtga ggaaacgacc attaatttgc accagcatgg ttaccgtatg
    10441 gttattggca tcgacgcgca gcggttggct gttcgaaagt ccgtcgtatt ccacttttgc
    10501 cgccgcattc tctttttgat cgcagccgct aagcccggca acagccagta agcagagtgc
    10561 agccagtcct tttttccatg acactgaata atgttgcgac atcgttctat ccttcatttt
    10621 ttgggtgagt aagtgggctg cggcgcgaag cagacagatc cacatgtttg tgtcgggcgt
    10681 agagttttgc gagctggacg agaataaaca gcgccaggcc cgccagacag agttgtaaaa
    10741 taaagcgcag cgtaatgcct tcattggcga gatcgcttgc catcacggta taaataacaa
    10801 taccgggcag ggtcgtgagt gccgaaataa gggtataagg ccagaaggcg atggtggtta
    10861 atccgtaagc gtaattttga atattgtaag gaaacaacgg gattaagcgg gtcagaataa
    10921 gaaaatcaat accgttacgc gcaatgcctt tttcaatggc ctggaaggta tggctatggc
    10981 ccacgtattt cagcagtaaa tcacgtccca gccagcgcgc cagcaggaat gagcacgagg
    11041 aggccagcgt tgcggcaatt aatgagagta gtgtccctaa gagcgggcca aaaactattc
    11101 ctccagcgat caccagtatg cttcctggta acagaaagag ggtggcaatg atgaataaca
    11161 gaatatagag gctataaccg aacaatccgc tctggcgaat gagtgtctgt aagtggggta
    11221 aatcggtgag cagatcgaat aaaccgaaag cgtggatagc gtaaatgacc agcgcaaaaa
    11281 taagacaggc aaacagaaag ttacgctcag cgttcatcgt ggagtttcct atatcaaact
    11341 tgataataga gaagtggtca gacaactaaa aacgcagtga cggtataaca aaggacggca
    11401 ggcaacaaat cattactgac agtttgttga tttttctcaa ggttaattct cctgacccag
    11461 tttgagccag gagagctgct aaattagcgg cggaaggtcg cccagacggg ggcgtgatcg
    11521 gacggttttt ccatgctgcg aatttcatag tcgatgccgg tttctacgca acattctgcc
    11581 agcggttggc tggcgagcag caggtcgatg cgcagaccac ggttatcgtc aaaacctttt
    11641 gagcggtaat caaaccatga gaaacgatct gctgtttgcg gattagcatg gcggaaggta
    11701 tcgaccaacc cccagctcat cagcctgtcc atccattcgc gctcttccgg caggaaagag
    11761 catttaccgg tacgcagcca gcgcttacgg ttttcttcgc caatgccgat atccagatct
    11821 gtagggctga tattcatatc gcccataatc agtaccggat tatcacgttt gagttcggtt
    11881 tccaggtagt tttgcagatt ctgataaaac tgcgcttttg ccgggaattt tatcggatgg
    11941 tcgcggcttt caccctgcgg gaagtaaccg ttgatcacgg tgacattacc cagcagtgag
    12001 gggatttccg ccataataat ccgccgctgc gcctcttcgt cgtcaccggg aaagccgcga
    12061 cgcacggcaa tcggcgtctc tttggtcagc agcgccacgc cataatggcc tttctgcccg
    12121 tgataaaaca cgttgtagcc gagcttcgcc acctcttcga gcggaaacat atcgtcatga
    12181 acttttgtct cctgcaggcc aatcacatcc ggttggtgct tttcgacgat ggcttcaagc
    12241 tggtgaggtc tggcgcgcag gccgttgata ttaaaagaga caaatttcat agtcgctgcc
    12301 atttattcag ataaagagtg cgtggatggt agcagaattt cgcgttgctt accgcctgtt
    12361 gttaagtgat ttgatcgatg gttgttaatg atgtcaacga tggcgcaaaa aatgcccgct
    12421 tttggtgcgc gctgcgtcag aatggcgcag taatttccag taaatttcgt caatgatcac
    12481 aattccagaa ttatatttta cttctgcata acattgcgtt ttttattctt tttattcacc
    12541 cacatcgcaa acgtattcac tttatatgca ctttaaatgc atatggtttg gttataactc
    12601 cttgatttcc agttagcctc cgccgtttat gcacttttat cactggctgg cacgaaccct
    12661 gcaatctaca tttacagcgc aaacattact tattgt
//
LOCUS       DAZGAN020000134        12554 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGAN020000134 DAZGAN020000000
VERSION     DAZGAN020000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.134"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(822..1817)
                     /locus_tag="ACHKYO_RS21075"
                     /old_locus_tag="pgaptmp_004215"
     CDS             complement(822..1817)
                     /locus_tag="ACHKYO_RS21075"
                     /old_locus_tag="pgaptmp_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HGU4430960.1"
                     /translation="MGLFCLRATPGAYQPQSLSQMVFQESNNRDVARKYAEYITSVPL
                     RRFVAEKVREYCGDLPLSIFDGACGSGQLEQFLTVGSLYAVDIQTSPLLHIPDNFPDA
                     TVKTVNENFFNAIDDVSDTDTFDCVIMNPPFSLQYKAQSADIREKISAMMPYKKTSGV
                     LDDAFYVLASMKARFSFFLCFPGVGYRRGELAMRQYFGNRVVELVQISGGFADTSVSV
                     LFVIIDNEKQSDDVVVYGVGFNGETCKKSAPVTEKINTDNWNRPYIAEEKEVIDIVQV
                     TAELLVRQTRRRKLEDEHNAFVWSLMSDDQRTEVSRFLSVHGLFFDAGSGCVHIR"
     gene            complement(1843..1919)
                     /locus_tag="ACHKYO_RS21080"
                     /old_locus_tag="pgaptmp_004216"
     tRNA            complement(1843..1919)
                     /locus_tag="ACHKYO_RS21080"
                     /old_locus_tag="pgaptmp_004216"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1883..1885),aa:Arg,seq:tct)
     gene            complement(1933..2009)
                     /locus_tag="ACHKYO_RS21085"
                     /old_locus_tag="pgaptmp_004217"
     tRNA            complement(1933..2009)
                     /locus_tag="ACHKYO_RS21085"
                     /old_locus_tag="pgaptmp_004217"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1973..1975),aa:Arg,seq:tcg)
     gene            complement(2017..2092)
                     /locus_tag="ACHKYO_RS21090"
                     /old_locus_tag="pgaptmp_004218"
     tRNA            complement(2017..2092)
                     /locus_tag="ACHKYO_RS21090"
                     /old_locus_tag="pgaptmp_004218"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2057..2059),aa:Ile,seq:cat)
     gene            complement(2133..3181)
                     /locus_tag="ACHKYO_RS21095"
                     /old_locus_tag="pgaptmp_004219"
                     /pseudo
     CDS             complement(2133..3181)
                     /locus_tag="ACHKYO_RS21095"
                     /old_locus_tag="pgaptmp_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(3332..3529)
                     /locus_tag="ACHKYO_RS21100"
                     /old_locus_tag="pgaptmp_004220"
     CDS             complement(3332..3529)
                     /locus_tag="ACHKYO_RS21100"
                     /old_locus_tag="pgaptmp_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4430961.1"
                     /translation="MLKQQDMTETARVVFNELSATEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(3549..3746)
                     /locus_tag="ACHKYO_RS21105"
                     /old_locus_tag="pgaptmp_004221"
     CDS             complement(3549..3746)
                     /locus_tag="ACHKYO_RS21105"
                     /old_locus_tag="pgaptmp_004221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430962.1"
                     /translation="MMTSLLPLCKRHLNGAFVVFIKRSPPLAGFFMPEKRHRALNALV
                     VANTGLFSLLAFLTRVIGVSR"
     gene            complement(3872..4273)
                     /locus_tag="ACHKYO_RS21110"
                     /old_locus_tag="pgaptmp_004222"
     CDS             complement(3872..4273)
                     /locus_tag="ACHKYO_RS21110"
                     /old_locus_tag="pgaptmp_004222"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGU4430963.1"
                     /translation="MVLKYQPSVRSVLMCDFRGMVVPEIVKVRPVVVVSRNRHNNQLV
                     TVVPISTTEPIPRRDCHHELSENPIPGNEHITCWVKCDMLMTVSLSRLDRIKTRTWEG
                     RNYIVPMIAEDEFENIKRAVLHGIGMAYLYR"
     gene            complement(4467..5156)
                     /locus_tag="ACHKYO_RS21115"
                     /old_locus_tag="pgaptmp_004223"
     CDS             complement(4467..5156)
                     /locus_tag="ACHKYO_RS21115"
                     /old_locus_tag="pgaptmp_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU4430964.1"
                     /translation="MNNQYLQFVREQLMIATADLSVSIKGQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIALVSPVEFSTSSWRRAVLELEAHQK
                     AWLLWCYGGNICWEYQIAITQWAWNEFNTQSGTRKIAGKTLERLKKLIWLAAQAVKAE
                     LFGGEGYEYRDLASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQNIAKLD"
     gene            complement(5149..5511)
                     /locus_tag="ACHKYO_RS21120"
                     /old_locus_tag="pgaptmp_004224"
     CDS             complement(5149..5511)
                     /locus_tag="ACHKYO_RS21120"
                     /old_locus_tag="pgaptmp_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4430965.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     QLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     PVSGGRLEIRITEVGVHE"
     gene            complement(5524..6573)
                     /locus_tag="ACHKYO_RS21125"
                     /old_locus_tag="pgaptmp_004225"
     CDS             complement(5524..6573)
                     /locus_tag="ACHKYO_RS21125"
                     /old_locus_tag="pgaptmp_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4430966.1"
                     /translation="MRVLLRPVLVPELGLVVIKPGRELLPVFHRGRVLVEPEPKSMRS
                     LPSGVVPVVCQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQVEL
                     IFRFLDHAFATGVLG"
     gene            complement(6575..6847)
                     /locus_tag="ACHKYO_RS21130"
                     /old_locus_tag="pgaptmp_004226"
     CDS             complement(6575..6847)
                     /locus_tag="ACHKYO_RS21130"
                     /old_locus_tag="pgaptmp_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430967.1"
                     /translation="MTQHIKSHNSEADPEIKRGWRFRTPQYGWFHYLFCTTDEADMLQ
                     EAYLRRGVRVERSLNVDRLTWTVSVYLPVRAHLPRTNACYRQRVWR"
     gene            complement(6970..7314)
                     /locus_tag="ACHKYO_RS21135"
                     /old_locus_tag="pgaptmp_004227"
     CDS             complement(6970..7314)
                     /locus_tag="ACHKYO_RS21135"
                     /old_locus_tag="pgaptmp_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HGU4430968.1"
                     /translation="MKKVLIAALISGVSFGVFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIIKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            complement(7436..7591)
                     /locus_tag="ACHKYO_RS21140"
                     /old_locus_tag="pgaptmp_004228"
     CDS             complement(7436..7591)
                     /locus_tag="ACHKYO_RS21140"
                     /old_locus_tag="pgaptmp_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4430969.1"
                     /translation="MKQQKAMLIALIVICITVVVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(7882..8235)
                     /locus_tag="ACHKYO_RS21145"
                     /old_locus_tag="pgaptmp_004229"
     CDS             complement(7882..8235)
                     /locus_tag="ACHKYO_RS21145"
                     /old_locus_tag="pgaptmp_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4430970.1"
                     /translation="MIDRTKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMCKAGN
                     SPVTPDGWISCSERMPPVGVDVQLFVSSTGEQFTGFNLDNTGDFQYAQCFGAPIVCEA
                     THWMPLPEPPKEVRQ"
     gene            complement(8250..8600)
                     /locus_tag="ACHKYO_RS21150"
                     /old_locus_tag="pgaptmp_004230"
                     /pseudo
     CDS             complement(8250..8600)
                     /locus_tag="ACHKYO_RS21150"
                     /old_locus_tag="pgaptmp_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8602..8946)
                     /locus_tag="ACHKYO_RS21155"
                     /old_locus_tag="pgaptmp_004231"
     CDS             complement(8602..8946)
                     /locus_tag="ACHKYO_RS21155"
                     /old_locus_tag="pgaptmp_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4430971.1"
                     /translation="MSGINYQALREAAQNYQSMLAWYQEKPDSPNAEQDCDAALAAFK
                     REIRHREVDIIADLLDELEEAKQRIDEQESRIVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTAAGIRIEGE"
     gene            complement(8933..9238)
                     /locus_tag="ACHKYO_RS21160"
                     /old_locus_tag="pgaptmp_004232"
     CDS             complement(8933..9238)
                     /locus_tag="ACHKYO_RS21160"
                     /old_locus_tag="pgaptmp_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGU4430972.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHKAEEALKREGHTVLNPSVLPD
                     GLTQPHYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEWD"
     gene            complement(9235..9516)
                     /locus_tag="ACHKYO_RS21165"
                     /old_locus_tag="pgaptmp_004233"
     CDS             complement(9235..9516)
                     /locus_tag="ACHKYO_RS21165"
                     /old_locus_tag="pgaptmp_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4430973.1"
                     /translation="MILRQCAGTIKVKSIGALIGRTEAAVRTKARELGISMMLRGDFH
                     QSAKYSQRDIEQARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVQA"
     gene            complement(9549..10265)
                     /locus_tag="ACHKYO_RS21170"
                     /old_locus_tag="pgaptmp_004234"
     CDS             complement(9549..10265)
                     /locus_tag="ACHKYO_RS21170"
                     /old_locus_tag="pgaptmp_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4430974.1"
                     /translation="METVFDALKALKKASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVKETT
                     TQEEIKTESVAVTVQSQPSFTRKHQDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            complement(10299..10760)
                     /locus_tag="ACHKYO_RS21175"
                     /old_locus_tag="pgaptmp_004235"
     CDS             complement(10299..10760)
                     /locus_tag="ACHKYO_RS21175"
                     /old_locus_tag="pgaptmp_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4430975.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGTNPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWTKHVGNGMSVPPVRRQLEGAKHPKGPTPIEWLKQEYERRKAAGFI"
     gene            complement(10753..11763)
                     /locus_tag="ACHKYO_RS21180"
                     /old_locus_tag="pgaptmp_004236"
     CDS             complement(10753..11763)
                     /locus_tag="ACHKYO_RS21180"
                     /old_locus_tag="pgaptmp_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HGU4430976.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTELKELNPTHNVHE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAVQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            complement(11850..12287)
                     /locus_tag="ACHKYO_RS21185"
                     /old_locus_tag="pgaptmp_004237"
     CDS             complement(11850..12287)
                     /locus_tag="ACHKYO_RS21185"
                     /old_locus_tag="pgaptmp_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4430977.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDSGSSGESVVVH"
     gene            complement(12271..12552)
                     /locus_tag="ACHKYO_RS21190"
                     /old_locus_tag="pgaptmp_004238"
     CDS             complement(12271..12552)
                     /locus_tag="ACHKYO_RS21190"
                     /old_locus_tag="pgaptmp_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012006457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HGU4430978.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQVLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTQTLTQPLSRSLIHENQA"
BASE COUNT         3020 a         3553 c         2736 g         3245 t
ORIGIN      
        1 tctcaccatg cgggcttttt ttatccataa gaaagcccct ccggagaggg gctgaagagt
       61 agcgctatgt accgttcatt acatgatgcc gggtgcctcc cggtgagttc agcccggtgc
      121 caccgaaccc gcgtcattct cgctaacgat catcagagac cattccgtac accagccgcc
      181 cctccgcaca gggggattca tcatgcagaa attttttaac aaattctcag tcgggaagac
      241 aaccttcaac tgactgaatt gtgagcaaca tagcatttaa cggggaacct gttttctgca
      301 gtaaaaaggc ccaccggagc ggacgggcct ggaaggatag cggtcatgtg atgccggttt
      361 cccggtaact cagcaccggt atctgagtca acgttttctc tactgggtca tttccgatac
      421 gccctgcctg ctgacaggct ttcatcacat ctgaaaatat agcaccctga ctgatactgt
      481 agtacccaag gttccagaaa ctgtgatgta tccggcacag aaaagcccct ccggagaggg
      541 gctgacgctg cgtatctgta tcatcatgcg catggtgccg ggtgcctccc ggtgagttca
      601 gtatcagcac ctgaacccgc acagaaagga taagggatgt aaccaaacac ctgtcgctga
      661 tatgcccctc cgctcagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa
      721 tcagacaact gtcaaccgtc tgaattgtga gacatttaaa attttcgggg caacaggaag
      781 acaatcatca cgacaccggc aaccagcgac cggcaccaga tttaacgtat gtgcacacaa
      841 cctgaaccgg catcaaaaaa caaaccgtgc actgacagaa aacgggaaac ctccgtccgc
      901 tgatcatcac tcattaagga ccacacaaag gcgttatgct catcttcaag tttccgtctt
      961 cttgtttgcc ttacaagcaa ctcagccgtc acctgtacaa tgtcaatgac ctccttctct
     1021 tcagcaatgt acggcctgtt ccagttgtct gtgttaatct tctccgtcac cggcgctgac
     1081 tttttgcaag tttccccatt aaacccgacg ccataaacaa caacatcatc gctttgcttt
     1141 tcgttatcaa taatgacgaa taatacagaa accgatgtat cagcgaaccc gcctgaaatc
     1201 tgaaccaact caacaacgcg gttgccaaaa tactgacgca ttgccagctc accacgcctg
     1261 taaccaaccc cgggaaagca caaaaaaaat gaaaatcttg ccttcatgct cgccagaaca
     1321 taaaacgcgt catccagaac accggaggtc tttttataag gcatcatcgc tgaaatcttc
     1381 tccctgatat ccgcgctctg tgctttgtac tgtagcgaaa aaggcggatt catgatgaca
     1441 caatcgaacg tgtccgtgtc actgacatca tcgatggcgt tgaagaaatt ttcattgacc
     1501 gtcttcactg tggcatccgg gaaattatcc gggatatgca acaacggaga ggtctgtata
     1561 tccacagcat acagacttcc caccgtgaga aactgctcca gttgcccgga gccacacgca
     1621 ccatcaaaaa tactcagggg cagatcacca caatattccc gcactttttc agcgacaaac
     1681 cgacgcaaag gaacggacgt gatgtactct gcgtattttc tggcgacatc gcgattattg
     1741 gactcctgaa aaaccatttg tgaaaggctc tgtggttgat atgcgccggg cgtggcgcgg
     1801 aggcaaaaaa ggcccacaaa agcgagccag gaaaaataag tgtggtgcgt tgtactggat
     1861 tcgaaccagt gaccgattgc ttagaaggca attgctctgt ccggctgagc taacaacgca
     1921 gtgtacagat aatggaccgc catcgaggac tcgaaccccg cgcaaccagc ttcgaaggct
     1981 ggcgctctat cccgatgagc taatggcggt atgtgatggt ggcccttgct ggatttgaac
     2041 cagcgacctg gcgattatga gtcgctcgct ctcaccactg agctaaaggg ccgggcgcag
     2101 gataataacg gtacgtaact aatcctgcaa tatcatccgt tctgactggc taaatcctga
     2161 acttccctga ccgtctgctc aaaacgttca gtctccagct caacgccagt tgcacgacgc
     2221 cccagcgcca tcgctgcttt gactgtcgaa cccgacccca tgaaaaaatc tgcaaccagg
     2281 tcacccggac gactgctcgc gctgattatc tgctgcagca tttctgccgg tttttcgcac
     2341 ggatgtttcc cgggatagta ctgcaccggt ttatgcgtcc acacatcggt gtacggcacc
     2401 tgcgccgtca caccgaaata ccgccgcaga tgcttatatt cactctgcag ttccgtatag
     2461 tgtcggttca gttcactgta tgtgctgacc agttggtgat gtggcttttc cagttctccg
     2521 cgctgatgct tctcttctgc cacccgggca aacagcgcct gtaatttcag ataatcgctt
     2581 tcgtccggta gctggcactg aaccagtgcg acaccatgtt tttctttcct gtggcatcag
     2641 caatctgttt tgccgttatc cccagggcag cacgtgcatc acgaaagtaa gcaatcagcg
     2701 gggccatcac atgctgtttc agtgccctgc ccttcgcctc atacccggca tctttcggac
     2761 gatacggccc ctgataatgt tccgcgaaca gaatgcgctc tgtggcgggg aaatacgccc
     2821 tcaggctttc cttgttgcac ccgttccagc gtccggacgg cttcgcccag ataatatggt
     2881 tcagcacact gaagcgttca cgcatcatga tttcaatgtc agatgccagg cgatggccac
     2941 agaacaggta aagacttccg gcaggtttca gcacccgcca gaactgcgcc agacactggt
     3001 ccagccactt caggtaatca tcgtcgccct tccactggtt atcccagccc tcaggcttca
     3061 ctttaaagta cggcgggtcc gtgactatca ggtcaacaga attttcgggt aacgaccgga
     3121 taaattccag gcagtcggcg ttgattaact cacaactgta tatttttaca gcattaagca
     3181 tggatcatta agcctgtctc tgataggctc attctgcttt tgcgcaaagc agtgggcctg
     3241 aggtttgctt gtgatccgga cgcatgagca gatggctggt gagtgcccct aacacccacc
     3301 agccgcccat ttaccacaaa taaaaaagcc ttcaggactg aaggcgtctg taacaaccga
     3361 actgatagtc tgccagaccc gccataacaa gctgggtcag tattaactgg cagcgttcac
     3421 gtgaaagata agtattctgc gcaatctccc cgactgtcgc cggttcggtg gcacttaatt
     3481 cattaaacac cactctggcg gtttctgtca tatcctgctg tttcagcatg tctttttcct
     3541 ttttccggtt aacgtgacac accaataact cttgtcaaaa aagccagcaa gctgaaaaga
     3601 ccagtattcg caaccaccag cgcgtttaac gccctgtgtc gtttttcggg cataaaaaaa
     3661 cccgccagtg gcggggatct tttgataaaa acgacaaagg caccattaag gtgcctttta
     3721 cacaggggca gcaggcttgt catcacctta ttgctactcc tggcttccag ttcggaagtc
     3781 tcacaagtcc catcagggta cagcgacagt atcagtcaac acactatgtg tgtcaatata
     3841 aattcatatc gaattcgata atcgttttcg attatcgata cagataagcc atccctatcc
     3901 cgtgcaatac cgcccgttta atattctcaa actcatcttc cgcaatcata ggaacaatat
     3961 aatttcgccc ctcccaggtt ctagttttta tgcgatccag tcggctcaat gaaaccgtca
     4021 tcaacatgtc acattttacc caacaagtaa tatgctcatt accagggatg gggttttctg
     4081 ataactcatg atggcaatct ctgcgaggaa tcggttcagt agtgcttatt ggtaccactg
     4141 ttaccaattg attgttgtgt ctgtttctgg acacgactac cactggcctg accttgacta
     4201 tttccggaac aaccattccc cgaaaatcac acattaaaac agaacgaaca gacggctgat
     4261 actttaacac cataaatctc tttgcctgat aatgacgact tgtgaactcc actacaaaca
     4321 aaatgattct gctgaacagg cagtttcgca ctgtacatta taacctgtta agccaacaaa
     4381 aaaacccgct cggcggcggg gtttgcttac tttgccatcg cgtacaaaat cggcaaaata
     4441 tcagatttac atgaaatata tgctttttaa tccagttttg caatattttg ctgtgaaaat
     4501 gtggtctttt gttttgaacg tgttttcgtt aaaagcaata aagcttggct atcaagctgt
     4561 agaaaaatgt gcttcattgc aacccagcgt tcagtaaatg tctcagacca gttttttgat
     4621 gtcactccca ccagtgatgc cagatcccgg tattcataac cttccccacc aaaaagctca
     4681 gcttttaccg cttgcgccgc cagccagatc aacttcttca gacgttccag cgttttccca
     4741 gctatttttc tggtaccgga ttgagtatta aattcattcc acgcccactg cgttatcgca
     4801 atctgatact cccagcaaat gtttccgccg taacaccaca acaaccacgc cttctgatgc
     4861 gcttccagtt ccagaactgc acgtcgccat gatgatgtcg aaaactcaac cggactgacc
     4921 agagcaattg acgtcccttt cgccagtgat tgtttacccg ggatcggtgg gttatcccgc
     4981 gtgatcattt ttccggttac ctcatcgcgg taacgaattt ttttgcgtct gtaacgccct
     5041 gtatcgaaca gtgcattttc ctgccaggct tccagttggc cttttatcga cacactgaga
     5101 tccgcagtgg caatcatgag ttgctcacga acaaactgta aatactggtt attcatgcac
     5161 acccacctct gtaattctta tctccagccg tccaccagat actggctggc cacgtacaat
     5221 attgatttca tcaaactgct catcgtccat taacaaccct gcatgcgtca gcgcatccag
     5281 cggcgctttc aggatattat ccaggtcacg gcggcgctta tccggtggct ctgcaatcac
     5341 ctttatcgcc agccttccgg acaggcttaa tttcagctgc tgctggcgaa caataagcgc
     5401 cactgcccgg cgatagcgct ttccctcctc agatataaaa tatgtgctgc cacgacgtcg
     5461 ccagtaagta ttcaccgtcg gcggataagg caaaacaaac tctatacgca tcagtaacct
     5521 cttttaccca agcacgccgg ttgcaaaggc gtgatcaaga aaacgaaaaa ttaactcaac
     5581 ctgagagccg tactttttct caaactccag cgggtctgca tgaagttcgt tgtgatgttc
     5641 ccggcacaac ggtagcgtga aaatatcgtg ggcctttgtc cccattcccc cctgaccatg
     5701 accaatcagg tgatgcggat cgtcagcagg cttaccacaa cacgcacacg gctgtgtctt
     5761 tacccagcgc gtgtatttct cattaaccca acggcgacgt ttaggccgct tcatgaacga
     5821 ttcaggagac tccggatcaa cagcgatact gacaaccgtt tttttctgtg gtggattttg
     5881 ttgctggtgg acgtgaagtg gcagcgcaat attttttgtg cgctgcttca gtatgctgat
     5941 ggctgtctgt tctcccggta cgatgtcact ctcacggtat acggagcgga ttttttccgc
     6001 tggtaatccc agcgaacgac gcgctactgc ctcaggtaat gcatccacca cctgattgca
     6061 ggccgcccac caggataatt cggccagcga taactccctc tcctgcgtac cgcttattgc
     6121 atgacggatg acgtcaatca tccaggcaac cagattctgc tgagcaagtt gatcgagtga
     6181 ttctgatgtc tggtcgcgca gctggttgtc acagtgccag cacaacacca tcgcgccggt
     6241 accataacgg tgaatgacgg tttcgctgtg atgataatcg ccatgtggcc actggcagga
     6301 tttcacgtga cgtaataacc agtcagacag cgcaccagca ccgccagcgg cacgaatcac
     6361 ccgttcattg ctgaaaaatg gcagtaatga tttatcctca gccagcggct ggcaaacgac
     6421 aggaacaacc ccggacggca aactccgcat gcttttgggt tccggctcca ccagcaccct
     6481 gccgcgatga aaaaccggca gtaattcgcg accaggctta atgaccacca ggccaagttc
     6541 cgggaccaga acaggtcgaa gtaatacccg cacattatct ccagacacgc tgacggtagc
     6601 aggcatttgt tcgtggcaga tgtgcgcgaa caggaagata tacagaaacg gtccaggtca
     6661 gacgatcaac gttcagactc cgctccacac ggacaccgcg acgcagatac gcctcttgaa
     6721 gcatatctgc ctcatcggtc gtacagaaca gatagtgaaa ccagccatac tgaggcgtac
     6781 gaaaacgcca cccccgctta atttccgggt cggcttcaga attgtgggat tttatgtgtt
     6841 gtgtcatcgg attctccggt gacagcaggt gtcagttgtt caggctgacg gcgcgaattg
     6901 taagtcaata cgcctggaat gtacaaacag aaaacccgtc agtaagacgg gcttagcaag
     6961 ccgggacggt tactttaata atttcagtgc ctttacatca acttcaacac tgctcaggtc
     7021 tttatcaatt tcaccctcaa ttcttacttt gtctttcgga gaaacattct gcccggccca
     7081 tacgctgtca tcaatatccg tgacaattgt cccgctattg tcacgaaact cataacgttc
     7141 atcacccact tttttaatga tgctcccttc aaggataacc catgcatcat ccttcagttc
     7201 ttttgcctgc gctactgttg aacgctctgc ttcaggcccc tggaaaccac cctgctgtgc
     7261 aaaaacgcca aaagacacac cggaaataag tgctgcaatc aatacctttt tcattcatag
     7321 tcctctttca gaaatgaaca ttcaaacagc attttcagta tggtaaagcg cgggtgcgtt
     7381 gaggatgcct gacacatcag aggtggcgag ggatttctcc ctcgcctggt cactcttact
     7441 tctcagattc gtagtctacg aagacagcaa cctccgtctg gccggttcgg attcgtacct
     7501 cgcagaggtc tttcctcgtt accagtgccg tcacaacaac ggtaatacag atgacgatca
     7561 gggcgattaa catcgccttt tgctgcttca tagcctgctt ctccttgcct ttcggcacgt
     7621 aagaggctaa cctacgtttg tgaagcatag attgggcctc agattaatgt taagcgtcct
     7681 gcaagacgcg taatgttaac tggggctttt ctctgtctgc cttacggcgg catgcccgag
     7741 gcagacagcc tcaagcaccc gcagcaattc tacttaactc ttctttcccc gcaaaccgtt
     7801 tttatcccca gcggcaaatc gaatacacca ccagcgccac cgccattgca ataccaacat
     7861 ttgagaaggc ctcaggccag ctcattggcg cacctccttc ggcggttctg gtagcggcat
     7921 ccagtgtgtg gcttcacaca cgataggcgc accgaaacac tgcgcatact ggaagtcacc
     7981 cgtattatcc aggttaaaac ctgtaaactg ttcccccgtg ctggacacaa acagttgtac
     8041 atcaacacca actggcggca ttcgctcact acagcttatc caaccatccg gagttaccgg
     8101 agaattgcca gccttgcaca tggcaatctc catgatttca accatatctc ctggtggaat
     8161 tttacaatgc tgaccaatat gcctctgctg cctggcatat tcgaggatgt gctccagttt
     8221 ggttcgatca atcatgattt atctccatta agcattgcag cacggcaatc gttccatcct
     8281 tcagcataat cactatgcgc aagaggccaa cctcttatgt attcacgcgg cgacttatca
     8341 ggcactacca gcgctggcgg gacggcaaat agatatccgc caaagtcagg aagctctcca
     8401 atggcctgta cgaacttttg tttgcctacg tcaactccta atgggtaatg agctataatc
     8461 tttgccactg gctctgcttc cagcgatgcc agtgcgataa gcgccagctc acgaatttca
     8521 ccgccgtcta tatcgtcaat gtcatcgcgg ccagaaatgt tagccagcca ttgcagtcgc
     8581 tctttggtaa tagtggtcat ctcactctcc ttctatgcga ataccagcag cagtcagtgc
     8641 ggcattgacc tcatcagcgt agtagtacgt taatccactt gatgatttag ccagcttgaa
     8701 tggttctggt aacttcacta tgcgggactc ctgctcgtcg atacgctgtt ttgcttcctc
     8761 cagttcatcc agcaaatcag cgataatatc cacttcacga tgacggattt cacgcttaaa
     8821 cgcagccaaa gctgcatcac aatcctgctc agcgtttgga ctgtctggtt tctcctgata
     8881 ccacgccagc atcgactgat agttttgtgc cgcctcacgc agcgcctgat aattaatccc
     8941 actcactggt tgcctcctgg aaaataactg catgccccag tttctccgcc agtgccagtt
     9001 ctgccttagc gcctgccgac cgctgccagc ctttcagcat ataaaccgca tccacacaac
     9061 gaatcattgc catgcaaata tccatgtaat gtggctgagt cagcccgtcc ggaagtactg
     9121 acgggtttaa aacggtatgc ccttcccgtt tcagtgcttc ttccgccttg tgaaatgcct
     9181 cgcggttgaa attttcatat cccgtcattg gaccggcgat ataaattctc accctcacgc
     9241 ctgaaccctc ctgtcgaaat aaacgtagtt attcactatg cgcagcggca tgcctaattt
     9301 tctggcaatt tcccttcttt gcatgcctct ctgatgcagt tgccgcgcct gctcaatatc
     9361 acgctgagaa tattttgctg actggtgaaa atcaccacgt aacatcatgc tgatgcccag
     9421 ctcccgtgcc ttcgttctca ctgccgcttc agttcgtccg ataagtgcgc caatgctttt
     9481 tacctttatt gttcccgcgc actgccggag tatcagaatt tcagcccggc accacgtctt
     9541 ccacccactc accgctgctg ttctctggtg gcggtaatat cccggagaat atcccggcac
     9601 ttgttcagct cccgcagcgc ggcgcagaca cgctcccact tctgaacctg accttttgcc
     9661 cggcgcagct cgcggttggc cacatgcagc gatggtaaaa tcaaaccatc ctgatgcttt
     9721 cttgtgaacg acggctgtga ctgcactgtg accgccacac tttcagtttt aatttcttcc
     9781 tgtgtcgtcg tttccttgac tggtaacgca acacctgctg gctgaggaaa ggctttacca
     9841 tccgtttccg ctacggatgc agcttccggc tctgccggta aatcagcgcc cggtatgcag
     9901 taacgaaatt taccgccctg attcacgcga atcagacgcc ctttgctgat tgccatggcc
     9961 agcgatgaat tcgcccggcg ggaggtaatc ccgaacatca gtgccagctc atccgccgtt
    10021 tgtgggccat gttgttcaat cgcctcagtc agcatttgcg ctgtcacttt cggtaccggt
    10081 gacactggtt cactttcacc agcctgaatc agccaccaca tcgacccctt gttatccgct
    10141 tcaccacggc gcttcagttt ccacagttcg ttgaccgcat cttcacggct gattccaagg
    10201 cgggccgcca ctacctgtga agaggctttt ttcagtgctt tcagtgcgtc aaatactgtt
    10261 tccattaaaa tttcctccga caaaatcgtt tctcagattc aaataaaacc agctgccttc
    10321 cggcgttcgt attcctgttt caaccattca attggcgttg gccctttcgg gtgtttcgcc
    10381 ccttccagtt gtcgtcgcac tggcggaaca ctcatcccgt taccaacatg ctttgtccat
    10441 ttcgtcagtt gccgttccgc aagtcgtttt aactcaccct gcgtcatctg gcgctcaatc
    10501 cctctggtac gcatttcgag gcagatgtgg tacagcacag gctgtggcca cgggtattta
    10561 tcactcccgt cgtatcgcca ggattcattg cgccagcgcc ggtactcttc catcacggca
    10621 tccaccgtaa gaccaaatgg atttgtccca ctctccgaaa tcagcgcaac aaactcagcc
    10681 aggtccgggg gccacgtttc acccgcccgg cagcggtcca tgcactgacg gcagacctgc
    10741 cggatttgct gttcagtcat cgcaccaatc tgggcaatcc agagcttcga aggtgcggcc
    10801 ccgttcttct gagtccagcg gttcgaatac acctccccca taagctccca cagcttccag
    10861 gccgtttccg ttgctgataa atccgttgtc acgttcccac tgttcgcgtg ctgcccggat
    10921 ttcctgaact gcccgtgatg ccgtgccacc tgatgctgca tggcttaccc ccttgctgac
    10981 tggttttacc tgtgccctga cgtgctgcac gtgacgggca aatttctgct cccactgaac
    11041 ctgcgtgaaa accttcccct ccgccatcca gtaatcccgg aatgcggcaa gctcagcagg
    11101 tgtaaattcc ggctcaggca gagccatacc ccacactgct gcccgttgtc gaaaatccgg
    11161 cgacggctgc cagacagtag tcatcgaaaa tttcccgatg ggttcgctca ggccgtccag
    11221 gtattcaggt tcggctgtct gcaacggcgc accatgcgac tcactggtcg gaatactctc
    11281 gcgttcatgc acgttatgtg tggggtttaa ttcttttagt tctgtatctt tattagttgc
    11341 ttttgtgttt gcgtcatgtt caaacacaac accaacattt gtttgaacgc ctgttaaatc
    11401 tctctcttgt tttgtttgaa catatgcttc ctttctgctt cttctggcct gaacagatgc
    11461 ttttccggcg gctgattttt tggttaattt ttccctgact gatgccagat cttcctcaat
    11521 ccgaagatgc acccattcat cgccgttatc gcaaaaaaac tcccgcaagg atggttccac
    11581 atcagcccat cgctcgttag tcagacggga aatttttgcc agcctgtttt taggtattgg
    11641 tttccctgtt tgccagtaat tgaacatcaa caacaaatac gcgccgtgct cctctgcgga
    11701 caaatgcatg gtgtcagcca ggtaatcagc tatgtacagt tgcatgtatg gtaatgcggc
    11761 cataattgcc tcatcttgtg acgaaccatc ctctggtgat attctgtgat tccccaatca
    11821 acagaatcag caggggtctg gcataaatat caatgcacca caacagactc gccggatgac
    11881 ccgctgtcgc tgaaatacgc tttccggtaa acggcctgga ctgcatcatc atgtgcatca
    11941 attgccgtgc tcaacgcttc ctgcgccgcc agtaatgcac ggcgctcaac agtgtcaaaa
    12001 atactgaggt gataacgcag ttcacgcgga agaacagtca ggatagccgg aattagtgcc
    12061 tgaatttttt caacagcatc aggcgtatcc ttttcaagcc agcgaaaaat attttgtatg
    12121 ttcaatccaa taccctctgt cgtagaggtg tcatgcattg gtggataagt catttcaagc
    12181 tcaaaatatg ctgtcgctat atcatcagcg atttttttgc gccctaccct cggataaagc
    12241 agccacgcat tcattgccat gcggatatgt tcatgcttga ttttcatgaa tcaagctcct
    12301 ggaaagtggt tgtgttaacg tttgggtatc ttccagctca ggccaaatgt tcatccaatc
    12361 aaaaggccgt agttgctgac gtgtaacttc accattactg gctcgctcaa taaggacaca
    12421 taacgatgcc cctaacactt gacctttact caatgctttt cttagataac cgatgctagt
    12481 accacactca catgcaaaca tacgctgttc atctgacgaa agagaattga gaaatattct
    12541 taattcttcc ataa
//
LOCUS       DAZGAN020000135        12470 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGAN020000135 DAZGAN020000000
VERSION     DAZGAN020000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.135"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..781)
                     /gene="allD"
                     /locus_tag="ACHKYO_RS21195"
                     /old_locus_tag="pgaptmp_004239"
     CDS             complement(<1..781)
                     /gene="allD"
                     /locus_tag="ACHKYO_RS21195"
                     /old_locus_tag="pgaptmp_004239"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HGU4430979.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRN"
     gene            1098..2765
                     /locus_tag="ACHKYO_RS21200"
                     /old_locus_tag="pgaptmp_004240"
     CDS             1098..2765
                     /locus_tag="ACHKYO_RS21200"
                     /old_locus_tag="pgaptmp_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU4430980.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            2775..4034
                     /locus_tag="ACHKYO_RS21205"
                     /old_locus_tag="pgaptmp_004241"
     CDS             2775..4034
                     /locus_tag="ACHKYO_RS21205"
                     /old_locus_tag="pgaptmp_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU4430981.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            4045..4860
                     /locus_tag="ACHKYO_RS21210"
                     /old_locus_tag="pgaptmp_004242"
     CDS             4045..4860
                     /locus_tag="ACHKYO_RS21210"
                     /old_locus_tag="pgaptmp_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU4430982.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            4857..5750
                     /locus_tag="ACHKYO_RS21215"
                     /old_locus_tag="pgaptmp_004243"
     CDS             4857..5750
                     /locus_tag="ACHKYO_RS21215"
                     /old_locus_tag="pgaptmp_004243"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU4430983.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQRLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSIGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(5887..6954)
                     /gene="purK"
                     /locus_tag="ACHKYO_RS21220"
                     /old_locus_tag="pgaptmp_004244"
     CDS             complement(5887..6954)
                     /gene="purK"
                     /locus_tag="ACHKYO_RS21220"
                     /old_locus_tag="pgaptmp_004244"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGU4430984.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6951..7460)
                     /gene="purE"
                     /locus_tag="ACHKYO_RS21225"
                     /old_locus_tag="pgaptmp_004245"
     CDS             complement(6951..7460)
                     /gene="purE"
                     /locus_tag="ACHKYO_RS21225"
                     /old_locus_tag="pgaptmp_004245"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGU4430985.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPYHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(7578..8300)
                     /gene="lpxH"
                     /locus_tag="ACHKYO_RS21230"
                     /old_locus_tag="pgaptmp_004246"
     CDS             complement(7578..8300)
                     /gene="lpxH"
                     /locus_tag="ACHKYO_RS21230"
                     /old_locus_tag="pgaptmp_004246"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HGU4430986.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(8303..8797)
                     /gene="ppiB"
                     /locus_tag="ACHKYO_RS21235"
                     /old_locus_tag="pgaptmp_004247"
     CDS             complement(8303..8797)
                     /gene="ppiB"
                     /locus_tag="ACHKYO_RS21235"
                     /old_locus_tag="pgaptmp_004247"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HGU4430987.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            8971..10356
                     /gene="cysS"
                     /locus_tag="ACHKYO_RS21240"
                     /old_locus_tag="pgaptmp_004248"
     CDS             8971..10356
                     /gene="cysS"
                     /locus_tag="ACHKYO_RS21240"
                     /old_locus_tag="pgaptmp_004248"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGU4430988.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(10392..10913)
                     /locus_tag="ACHKYO_RS21245"
                     /old_locus_tag="pgaptmp_004249"
     CDS             complement(10392..10913)
                     /locus_tag="ACHKYO_RS21245"
                     /old_locus_tag="pgaptmp_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4430989.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(11021..11233)
                     /gene="ybcJ"
                     /locus_tag="ACHKYO_RS21250"
                     /old_locus_tag="pgaptmp_004250"
     CDS             complement(11021..11233)
                     /gene="ybcJ"
                     /locus_tag="ACHKYO_RS21250"
                     /old_locus_tag="pgaptmp_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HGU4430990.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(11235..12101)
                     /gene="folD"
                     /locus_tag="ACHKYO_RS21255"
                     /old_locus_tag="pgaptmp_004251"
     CDS             complement(11235..12101)
                     /gene="folD"
                     /locus_tag="ACHKYO_RS21255"
                     /old_locus_tag="pgaptmp_004251"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HGU4430991.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
BASE COUNT         2855 a         3286 c         3510 g         2819 t
ORIGIN      
        1 aattacgccc tgcgtgtaaa tcgtcataca tcgaactaac ctgtcgcccg aacggtaagc
       61 cgagtaagac gcctgagagg acgtcaatca tcatcatcag gccataccct tttggcccgg
      121 cggcggggag cagagcatgt accgcgaacg gatcggttgt tggtgcaccg tttttatcga
      181 ccgcccaggt atccgggata gacatattac gcgagcgggc gtcgagcact tttccccatg
      241 cctgtacggt agtcgccata tcaaaggtaa ggatctcgtc gccttctccc ggcgcggcaa
      301 aggccagggg gttagtaccg tagtaaattt ccgcgccgcc aaacggcacc accattggat
      361 cggactggca catcgaaatg ccaatgaatc cggcgcgggc tgcctgctgc acaaaataag
      421 agattgcgcc gctgtgaccc atccggctga taccgaccac cgcaacgcca ttttgctggg
      481 cggttttgat ggcatgttcc atacccattt tcgccgcgac ctgtccggcg gcattgtcgg
      541 catgtaaaat tgccgagcac ggcccggttt cctcaagacg aaactccggt tcgcggttgg
      601 tgccgccttt tgaaatgcgt tccgcgtagt attccacgcg caccgcgcca tgagagtgga
      661 tccctctggc atcggcgtaa accaatactt cagccacggt tgcagcgtgc tcacgtttta
      721 acccagcctg gcagagttta ttctcaatta gctggtggag tgtttcccga ctgattttca
      781 tctgtcttcc tttttaacga cggtgtgaag catgactgca attaacatac agggaaaata
      841 tctggattat gtgatccaga caggcaaaaa aatatagtta gaatttattt gataatccgc
      901 tcgcttttaa cctggttttt aaaacaacca cgcttattaa aaaataacac gtaatagcat
      961 ggcggttatt tgaattcttt tgttaataat ccctgtgtga tattcatcac cttatttatc
     1021 cgttgtcatc gataccgtaa tcgccacatt aacactgctc gtgcaattgc catgggtgca
     1081 atttttaagg agttgttatg atccacgcct ttattaaaaa agggtgtttt caggattcgg
     1141 tcagtttaat gattatttca cgaaaactca gcgaatcaga aaatgttgat gatgtttccg
     1201 taatgatggg tacgcccgcc aataaagcgt tattagatac cacaggtttc tggcatgacg
     1261 attttaataa cgccacgccg aacgatattt gcgtggcaat acgtagcgaa gcggcggatg
     1321 cggggatcgc gcaggcgatt atgcagcagc ttgaagaggc gctaaaacaa ctggcgcagg
     1381 ggtcaggcag cagccagtcc ttgacgcagg tgcgtcgctg ggacagtgcc tgtcagaaat
     1441 tatccgatgc caatctggcg ctgatttcag tggctggcga gtatgcggcg gagctggcga
     1501 atcaggcgct ggatcgcaac ctcaacgtga tgatgttctc cgataacgtc acgctggaag
     1561 atgaaatcca acttaaaacc cgcgcgcgag aaaaaggctt gctggtgatg gggccggact
     1621 gcggtacgtc gatgattgcc gccacgccgc tggcttttgc taacgtgatg ccggaaggca
     1681 acattggcgt cattggcgct tccggtaccg ggattcagga gctgtgctca cagattgcgc
     1741 tggcagggga gggaattact cacgcgattg gccttggcgg gcgcgacctc agccgtgaag
     1801 tgggcggcat cagtgcgctg acggcgctgg aaatgctcag tgcagacgag aaaagcgaag
     1861 tgctggcatt tgtttcaaaa ccgcctgccg aagctgtgcg tctgaaaatt gttaatgcca
     1921 tgaaagcaac cggcaaaccg acggtggcgc tgtttttagg ttataccccg gcggtggccc
     1981 gcgacgagaa tgtctggttt gcctcctcgc tggatgaggc cgcacgcctg gcttgcctgc
     2041 tttcacgcgt tacggcgcga cgtaacgcga taacgcctgc cagcagcgga tttatttgcg
     2101 gtttgtatac cggcggtacg ctggctgccg aagcggcggg attacttgcc gggcaccttg
     2161 gcgtggaagc cgacgatacc catcatcacg gcatgatgct cgacgccgat ggtcaccaga
     2221 ttattgacct cggcgatgat ttctacaccg tcgggcgtcc ccatccgatg atcgacccag
     2281 ccttacgcaa tcagttaatt gccgatctcg gcgctaaacc gcaagtgcgc gtattgctgc
     2341 ttgatgtcgt gattggcttc ggtgcgaccg ccgatcctgc cgcctcgctg gtaagcgcct
     2401 ggcaaaaagc atgtgccgcg cgttcagaca atcaaccact gtatgccatt gccacggtga
     2461 caggcactga acgtgacccg caatgccgct cgcagcaaat cgccacgctg gaagatgcag
     2521 ggatcgcggt agtgagttcg ctgccggaag ccaccttgct ggcggcggca ctgattcgtc
     2581 cgctttcgcc tgccactcag caacacacac cgtcattact ggaaaacgtc gccgtgatta
     2641 acatcggatt acgcagcttt gcgctggagc tacaaagcgc cagcaaaccg gttgtgcatt
     2701 accaatggtc gccagtcgcc ggcggcaata aaaaactggc tcgtttatta gaacgtttgc
     2761 aataaggggt tcccatgttt acatcagtgg cgcaagccaa tgctgcggtt atcgaacaaa
     2821 ttcgtcgtgc tcgtccacac tggctggatg tgcaaccggc ttcttcactt atcagcgaac
     2881 taaacgaggg caaaacactg cttcacgccg ggccgccaat gcgctggcag gagatgaccg
     2941 gacccatgaa aggggcgtgc gtgggcgcat gtctgttcga aggctgggcg aaagatgaag
     3001 cgcaggcgct ggcaatactg gagcaggggg aagtgaactt cattccttgt caccatgtga
     3061 atgccgtcgg tccaatgggc ggtattactt ctgccagtat gccgatgctg gtggtcgaga
     3121 acgtgaccga cggcaaccgg gcgtactgca acctcaacga aggtatcggc aaagtgatgc
     3181 gttttggcgc ttacggcgaa gatgtcctga ctcgccatcg ctggatgcgc gatgtgttaa
     3241 tgccagtatt aagcgcggcg ctggggcgca tggagcgcgg tatcgatctc acggcgatga
     3301 tggcgcaggg cattacgatg ggcgatgagt tccatcaacg caatattgct tcctctgcac
     3361 tgttaatgcg tgcgctggcc ccacaaattg ctcgcctcga tcatgataaa cagcacatcg
     3421 ccgaagtgat ggatttcctc agcgtgaccg atcagttctt cctcaacctc gcgatggctt
     3481 actgcaaggc ggcgatggat gctggcgcga tgatccgcgc aggcagcatc gtcacggcaa
     3541 tgacccgcaa cggcaatatg ttcgggattc gggtaagcgg gctgggcgaa cgctggttta
     3601 ctgcgcctgt aaacactccg caaggtctgt ttttcaccgg cttctcgcag gagcaggcga
     3661 acccggatat gggcgatagc gcgattaccg aaacctttgg tatcggaggt gcggcaatga
     3721 tcgcagcgcc tggcgtaacg cgctttgtcg gtgcgggtgg catggaagcg gccagagcgg
     3781 tatctgaaga gatggcggaa atttaccttg agcgcaatat gcagttgcag atcccaggct
     3841 gggattttca gggcgcgtgc ctgggactgg acattcgtcg cgtggtagaa accggcatta
     3901 cgccactcat caataccggt atcgcccata aagaggcggg gatcgggcag attggcgcag
     3961 gcactgtgcg ggcaccgctg gcgtgttttg aacaggcgct ggaagcactg gctgaaagca
     4021 tggggattgg ttgaggaacg cgcaatgacg atcatccatc ctctgcttgc cagccgtagt
     4081 gcaccgaatt atcgccagtc ctggcggtta gcgggagtgt ggcggcgggc gattaacctg
     4141 atgacggaaa gcggcgaact gttaacgctg catcgtcagg gtagtggttt cggccccgga
     4201 ggatgggtgc ttcgccgcgc gcaattcgat gcgttatgcg gtggattatg cggcaatgaa
     4261 cgaccacagg ttgtggctca agggattcgc ctcgggcgtt tcacggttaa acagccacag
     4321 cgttattgtt tgctgcgtat tacgccgcct gcgcatcctc aaccgcttgc agctgcatgg
     4381 atgcaacgcg cggaggaaac cgggcttttc gggccactgg cgttggcggc aagcgatccg
     4441 ctgaccgctg aattacgcca gtttcgtcac tgttttcagg ccgcgctcaa tggcgttaag
     4501 accgactggc ggcactggct gggtaaagga cccggattaa cgccaagtca tgatgacacg
     4561 ttgagcggaa tgctgctggc ggcctggtat tatggcgctt tagatgcgcg ctctggtcgc
     4621 ccgttttttg cctgttccga caatcttcaa ctcgttacca cagcggtgag cgtcagttat
     4681 ttacgttatg ccgcgcaagg atatttcgcc tcgccactcc tgcactttgt tcatgctctg
     4741 agttgcccga aacgtaccgc tgttgcgatt gattcgctgc tggcgctggg gcatacgtca
     4801 ggggcagata cgctgctggg gttctggctt ggccaacaat tattacaagg aaaaccatga
     4861 aaacactggt tgtagctctt gggggcaacg ccttactcca gcgcggtgag gcgctgacgg
     4921 cggaaaatca atatcgcaat atcgccagtg ctgtacccgc gctggcacgc ctggcccgtt
     4981 cttatcggct ggcgattgtt cacggcaacg ggccgcaggt ggggctgctg gcgttacaga
     5041 atctggcgtg gaaagaggta gaaccgtatc cgctggatgt gctggtggcg gaaagccagg
     5101 ggatgattgg ctatatgctg gcgcagaggt tgagcgcaca gccgcagatg ccgcctgtta
     5161 cgacggtgct gacgcgcatt gaggtttcgc ctgatgatcc ggcgtttttg cagccagaga
     5221 aatttattgg tccggtttat cagccagaag aacaagaggc actggaagca gcttacggct
     5281 ggcagatgaa acgtgatggt aaatacttgc gccgggtggt ggcgtctccg caaccgcgta
     5341 aaattctcga cagcgaagcc atcgagttgt tgctcaaaga ggggcatgtg gtgatttgca
     5401 gtggcggcgg cggtgtgcct gtgacggaag atggggcagg gagtgaagcg gtgattgata
     5461 aagatctcgc cgctgcgttg ctcgccgagc agattaatgc cgatggactg gtgatcctca
     5521 ccgatgctga tgcggtatat gaaaactggg gaacgccgca gcaacgtgcc attcgccatg
     5581 ccacaccgga tgagttagcg ccatttgcca aagccgatgg ttcgatcggg ccgaaggtaa
     5641 cggcggtgag tggttatgtc agaagccgta gtaaacccgc gtggattggg gcgttatcgc
     5701 gaattgaaga gacgctggcg ggcgaagcgg ggacctgtat ttcgctgtag tgatgagagc
     5761 atccggcaat cggtgcacca tgcctgatgc gacgcttgac gcgtcttatc aggcctacaa
     5821 tgggtaccgg agcggtaagc cggataaggc gtttacgccg catccggcaa tcaaatgagc
     5881 acctgcttaa ctgaacttac tctgcgccca aatcacgccg ctggcatact ctggcggcag
     5941 cagcgggatt aaggcttcca gcgtcgcagt cagacgcgat gtgtcgctgt cggtcaaatt
     6001 cagatgcccc actttacgcc ccggacggac ttctttgtcg taccagtgca gatgcaccag
     6061 cggcaatttc agccagtcat aattcgcatc gctaccaatc agattgatca tcaccgacgg
     6121 attattcact actggttgcg gtaacggcag atcggtaatc gcccgcagat gcagctcaaa
     6181 ctggctgatg ctggcaccgt tttgtgtcca gtgaccgctg ttatgcacac gcggagccag
     6241 ttcgttgatc agcagacctt gcggggtgac aaaacactcc atcgccatca cgcccacata
     6301 gcccagctcc tgcataatcg ccgacagcat ctcttcggct tgcgcctgct gctgtgcgtt
     6361 ggcctgcgga aaagcgacgc tggtgcgcaa aataccgtcc tgatgcaggt tatgcgtcag
     6421 cggataaaac acggtgctgc catcaaagcc gcgcgcgcca accagcgaca cttcaccaga
     6481 gaagttaatg ccctgctcga caatacattc gccgtaacac tctgccggta actgttcggt
     6541 ttcattggcg cgtaaactcc attgaccgcg accgtcatag ccaccagtgc gacgcttaac
     6601 aatcgccagt tcacctaaac gatcaaacac cgcaggccac tcgctgcgat cggcaagtaa
     6661 ctgccacggc gcggtcggca ggtggagctt atcgaaaagc tgcttctgag tcagacggtc
     6721 agcaataatc gggaacacat cgcggttcac aaaggccgga tgacgcgcca gctcgcgggt
     6781 taatgcggtt tccggccagc gttcgatctc agcggtaatc acgctttgtt gaaaaggcac
     6841 cgccgccggt tcagcgtcca gcccgaccgg ccagacagca atgcctaacg gttcgcctgc
     6901 ctgacgcagc atacggccta actgcccgtt accgaggacg caaacctgtt tcatgccgca
     6961 cctcgcgggt ccgggttttc cagcacttcg tcggtctggg ctttgcgcca gtcattcaga
     7021 cgctggtgta gctctttatc gtgagtcgcg agaatttgtg ctgccagtaa tgccgcgttt
     7081 gccgcgccag ctttaccaat cgccagcgta cccaccggaa tgccgcgcgg catttgtacg
     7141 atggagtaga ggctatcgac accgctcagt gcggcgctct gtactggcac gcccagcacc
     7201 ggcaccagcg ttttggcggc aatcatgcct ggcaggtgcg ctgcgccacc tgcgcccgca
     7261 ataatcacct gataaccgtt ctcttcggcg ctttcggcga agctgaacag tttatcgggg
     7321 gtgcggtgag cagaaaccac ttcaacgtgg tacgggacat tcaggatttc gaagatttcg
     7381 gcggcgaact gcatggtagc ccagtcgctt ttggacccca tcacgatggc gacacgcgcc
     7441 ggattattgc gggaagacat gcgtcttaaa actcctgtgg tgcacaactc tcggctttag
     7501 agggcacaga gaatagcacg gaaagagagc aaggaaaacg gttgcgtggc tgtgaaatca
     7561 gcaaagttgc gggtttttta aaacggaaaa tgaatcagct caacgtcatc cgccgtgact
     7621 ttcaccatcg aaccttccgt atgccaggca ccgagtacca cgcgaaaagc aggttgctgg
     7681 ttggcgataa gttcatgtac cgccgggcga tgggtatgtc cgtggatcag ccattgcacc
     7741 tgatgttttt ccatcgcact gactaccgcg ttttggttaa cgtccatgat cgccagcgat
     7801 ttactgctgt tggcttcttt actgttcgct cgcattcgcg cggcaatgcg tttgcgcaca
     7861 aacaacggca gggcgaggaa tagcatttgc agccagggtt tgtggacttt ggcgcgaaaa
     7921 gcctgataac ccgcgtcatc ggtgcacagc gtgtcgccat gcataatcaa cacccggcga
     7981 ccataaagtt cgagcacctt ttcttccggc aataatgtca tgccgctttc acgggcaaag
     8041 cgtttgccga gcagaaaatc acggttgcca tgaatgaaat aacagggaac gccggaatcg
     8101 gacaccgctt tgatcgctgc cgccatctgg cgatggagtg ggttgggatc gtcgtcgcca
     8161 atccatgctt caaacagatc gccaagaata tacagcgcgt cggccttgcg ggcttccccc
     8221 gctaaaaaac gcagaaaacc ggcggtgatc gccggttctt ccacgcagag atgaagatct
     8281 gcaataaaga gtgtcgccac gattactcgc taacggtcac gctttcaatg ataacgtctt
     8341 cttttggcac gtcctggtgc ataccgctac gaccggttgc aacacctttg attttgtcta
     8401 ccacgtccat gccgtcaacc acttcagcaa acacgcagta gccccaacct tgcaggcttt
     8461 cgccagagaa gttcaggaag tcgttatcaa ccacgttgat gaagaactgt gcagttgcag
     8521 agtgcggagc ctgagtacgt gccattgcca gcgtaccacg ggtatttttc aggccgttgt
     8581 tggcttcgtt tttgatcggt tctttggtgg ctttttgttt catgcccggt tcaaaaccgc
     8641 cgccctgaat cataaagccg ttgataacac ggtggaaaat ggtgttgttg taaaaacctt
     8701 cgcggcagta gtccaggaag tttttaactg tttcaggtgc tttatcgtca aaagttttga
     8761 tgacaatatc gccgtgattg gtgtggaaag taaccatttt tgcatcctgt tccgtttgat
     8821 tggtgcttca acccagttcg ggtcatatat agggtggtgt tatagcataa ccgcacgatc
     8881 ggatcatcac gcaatgtatg ctgattcgcg cgggaaatat gggtattata cgcaactcaa
     8941 ttacccacac atgtctaaac ggaatcttcg atgctaaaaa tcttcaatac tctgacacgc
     9001 caaaaagagg aatttaagcc tattcacgcc ggggaagtcg gcatgtatgt gtgtggaatc
     9061 accgtttacg atctctgtca tatcggtcac gggcgtacct ttgttgcttt tgacgtggtt
     9121 gcgcgctatc tgcgtttcct cggctataaa ctgaagtatg tgcgcaacat taccgatatc
     9181 gacgacaaaa tcatcaaacg cgccaatgaa aatggcgaaa gctttgtggc gctggtggat
     9241 cgcatgatcg ccgaaatgca caaagatttt gatgctttga acattctgcg cccggatatg
     9301 gagccgcgcg cgacgcacca tatcgcagaa attattgaac tcactgaaca actgatcgcc
     9361 aaaggtcacg cttatgtggc ggacaacggc gacgtgatgt tcgacgtccc gaccgatccg
     9421 acatatggcg tgctgtcgcg tcaggatctc gaccagctgc aggcaggtgc gcgcgttgac
     9481 gtggtcgacg acaaacgcaa cccgatggac ttcgttctgt ggaagatgtc gaaagagggc
     9541 gaaccgagct ggccgtctcc gtggggcgcg ggccgtccag gctggcacat tgaatgttcg
     9601 gcaatgaact gcaagcagct gggtaaccac tttgatatcc acggcggcgg ttcagacctg
     9661 atgttcccgc accacgagaa cgaaatcgcg cagtccacct gtgcccatga tggccagtat
     9721 gtgaactact ggatgcactc ggggatggtg atggttgacc gcgagaagat gtccaaatcg
     9781 ctgggtaact tctttaccgt gcgcgatgtg ctgaaatact acgacgcgga aaccgtgcgt
     9841 tacttcctga tgtcgggcca ctatcgcagc cagttgaact acagcgaaga gaacctgaag
     9901 caggcgcgtg cggcgctgga gcgtctctac actgcgctgc gcggcacaga taaaaccgtt
     9961 gcgcctgccg gtggcgaagc gtttgaagcg cgctttattg aagcgatgga cgacgatttc
    10021 aacaccccgg aagcctattc cgtactgttt gatatggcgc gtgaagtaaa ccgtctgaaa
    10081 gcagaagata tggcagcggc gaatgcaatg gcatctcacc tgcgtaaact ttccgctgta
    10141 ttgggcctgc tggagcaaga accggaagcg ttcctgcaaa gcggcgcgca ggcagacgac
    10201 agcgaagtgg ctgagattga agcgttaatt caacagcgtc tggatgcccg taaagcgaaa
    10261 gactgggcgg cggcagatgc ggcgcgtgac cgtcttaacg agatggggat cgtgctggaa
    10321 gatggcccgc aagggaccac ctggcgtcgt aagtaattgc gctgttgccg gatgcgagtt
    10381 ttcgcatccg gttatcgcct gcgccaccac aacattccca taagcaccat ccccggcaac
    10441 cacacccaca tcaattcaga aattatcacc tgatgcccgt acggcgtggt gtaacgagac
    10501 aatgcaaacg gcgcgacttt aatcacctgc cagggagcga aaaagcgttc atctgaccac
    10561 ggccacagcc agccaacgcc cttaccgcca gtggttaccg aatccagcaa gctgtgcgat
    10621 agcaacgaga cggttaaaaa cagccagcag cgaatcagcc cagccctgaa ccatcggcgt
    10681 ccaatcagca cacataacag cgggacaaca aacgcaaaca ccagcgaatg ggtaaacccg
    10741 cgatgaccaa aaacattgcc gtaagcaacg ccaaatttaa acgacaatac gtcggcgtcg
    10801 ggcagcatcg ccaggatgat tccggcaaat aacagacgcg gagggatgac tttcgaaccc
    10861 aaccctaaac caatgcatag gggaacagcg gcgtgcgtaa taacggttgg catgatggtc
    10921 gcttcggcaa aatgtcgatg ctatcagcat ggatgaacgg ggcgtagagg gcaaaagtct
    10981 gaaaagagaa ccggcctgtt gatacaggcc gggaaaggga tcaggcaaca acctgtacgc
    11041 tgtgacctgc aaaactcact gtctgaccgg cgacgatttt gcagcgtttg cgcgtttcaa
    11101 ccgcaccgtc gactttcacc tggccttcgg caatcgcgat tttcgcctgc gcgccgcttt
    11161 cgctccagcc ttccagtttc agcaagtcgc acagctcaac gtgcggatgt ttacctaaag
    11221 aaaatgtcgc catgttactc atcctgtgga tcatgatatt caacgcacgc ctgtagcgtg
    11281 ttttcaatca gcgtggcaac cgtcatcggg ccaacgccgc cgggaacagg cgtaatgtat
    11341 gaggcgcgtt tagccgcgtc ttcaaacacg acgtcgccca caactttgcc attttccaga
    11401 cggttgatgc cgacatcaat cacaattgcg ccttctttga tccagtcacc aggaataaag
    11461 cccggcttgc cgacggcaac gatcaacaga tcggcatttt ctacgtggtg acgcagattt
    11521 ttagtgaaac ggtgagtcac ggtagtggtg caacctgcga gcaacagttc catgctcatt
    11581 ggacgaccga cgatattcga tgcgccaatc accacggcgt tgaggccgaa ggtatcaatg
    11641 ttgtaacgct caagcagcgt gacgataccg cgcggggtgc agggacgcag acgcggcgcg
    11701 cgctggcaca gacgaccgac gttataagga tggaaaccgt ccacgtcttt gtccggatga
    11761 atacgttcca gcactttgac gttatcaata cccgccggta acggcagttg aaccagaatg
    11821 ccatcgatgg tgttgtcggc attcagcgca tcgataagct ccaacagctc cgcttcgctg
    11881 gtggtttccg ggaggtcata agagcgggag acgaacccga cttcttcaca agccttgcgt
    11941 ttgcttgcga cataaatttg cgatgcaggg ttactaccca ccagcacaac ggccagtcct
    12001 ggtgcccgca gtccggctgc aatacgcgcc tgaacttttt gagcaacttc agagcgcacc
    12061 tgctgcgcaa tcgttttacc gtcaataatc tttgctgcca tcagagagag gattccatct
    12121 gttacgtagg tcgaagggga tgcgcctatt ttgtcagaag cggggcgcgc tgtcaggttt
    12181 cgtttcagat ttatcgcgtg aagcgacctc ttgcgaaggt taggcgcacc gtcgctgagg
    12241 tcgctgagat tgaaagcttc atttttcgtc catgatggcg ttgtaaatct ggaactgatt
    12301 tatttccttg tttaaggatt aagataattt aagaaatacc tgacaatata aaaagaattt
    12361 tcagcctggt aatttaccgc ttcaggtcta tatttgtgtt gaatatattt tgcgcggaag
    12421 tattcatcta acggggctct ctatttttta gaatagagtg catattttca
//
LOCUS       DAZGAN020000136        12379 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGAN020000136 DAZGAN020000000
VERSION     DAZGAN020000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.136"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..91
                     /locus_tag="ACHKYO_RS21260"
                     /old_locus_tag="pgaptmp_004252"
     CDS             <1..91
                     /locus_tag="ACHKYO_RS21260"
                     /old_locus_tag="pgaptmp_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4430992.1"
                     /translation="GHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(131..1351)
                     /gene="ydcO"
                     /locus_tag="ACHKYO_RS21265"
                     /old_locus_tag="pgaptmp_004253"
     CDS             complement(131..1351)
                     /gene="ydcO"
                     /locus_tag="ACHKYO_RS21265"
                     /old_locus_tag="pgaptmp_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HGU4430993.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGV
                     CYVVLNLIADRNRY"
     gene            1404..1940
                     /gene="sutR"
                     /locus_tag="ACHKYO_RS21270"
                     /old_locus_tag="pgaptmp_004254"
     CDS             1404..1940
                     /gene="sutR"
                     /locus_tag="ACHKYO_RS21270"
                     /old_locus_tag="pgaptmp_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HGU4430994.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2013..3974
                     /gene="rlhA"
                     /locus_tag="ACHKYO_RS21275"
                     /old_locus_tag="pgaptmp_004255"
     CDS             2013..3974
                     /gene="rlhA"
                     /locus_tag="ACHKYO_RS21275"
                     /old_locus_tag="pgaptmp_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HGU4430995.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLFKRKG"
     gene            complement(4066..4296)
                     /gene="yncJ"
                     /locus_tag="ACHKYO_RS21280"
                     /old_locus_tag="pgaptmp_004256"
     CDS             complement(4066..4296)
                     /gene="yncJ"
                     /locus_tag="ACHKYO_RS21280"
                     /old_locus_tag="pgaptmp_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HGU4430996.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4518..4694
                     /gene="hicA"
                     /locus_tag="ACHKYO_RS21285"
                     /old_locus_tag="pgaptmp_004257"
     CDS             4518..4694
                     /gene="hicA"
                     /locus_tag="ACHKYO_RS21285"
                     /old_locus_tag="pgaptmp_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HGU4430997.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            4740..5156
                     /gene="hicB"
                     /locus_tag="ACHKYO_RS21290"
                     /old_locus_tag="pgaptmp_004258"
     CDS             4740..5156
                     /gene="hicB"
                     /locus_tag="ACHKYO_RS21290"
                     /old_locus_tag="pgaptmp_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HGU4430998.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5235..6641
                     /gene="ydcR"
                     /locus_tag="ACHKYO_RS21295"
                     /old_locus_tag="pgaptmp_004259"
     CDS             5235..6641
                     /gene="ydcR"
                     /locus_tag="ACHKYO_RS21295"
                     /old_locus_tag="pgaptmp_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU4430999.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESLGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKFLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6886..8031
                     /gene="ydcS"
                     /locus_tag="ACHKYO_RS21300"
                     /old_locus_tag="pgaptmp_004260"
     CDS             6886..8031
                     /gene="ydcS"
                     /locus_tag="ACHKYO_RS21300"
                     /old_locus_tag="pgaptmp_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HGU4431000.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8049..9062
                     /gene="ydcT"
                     /locus_tag="ACHKYO_RS21305"
                     /old_locus_tag="pgaptmp_004261"
     CDS             8049..9062
                     /gene="ydcT"
                     /locus_tag="ACHKYO_RS21305"
                     /old_locus_tag="pgaptmp_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4431001.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9063..10003
                     /gene="ydcU"
                     /locus_tag="ACHKYO_RS21310"
                     /old_locus_tag="pgaptmp_004262"
                     /pseudo
     CDS             9063..10003
                     /gene="ydcU"
                     /locus_tag="ACHKYO_RS21310"
                     /old_locus_tag="pgaptmp_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9993..10787
                     /gene="ydcV"
                     /locus_tag="ACHKYO_RS21315"
                     /old_locus_tag="pgaptmp_004263"
     CDS             9993..10787
                     /gene="ydcV"
                     /locus_tag="ACHKYO_RS21315"
                     /old_locus_tag="pgaptmp_004263"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4431002.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10809..12233
                     /gene="patD"
                     /locus_tag="ACHKYO_RS21320"
                     /old_locus_tag="pgaptmp_004264"
     CDS             10809..12233
                     /gene="patD"
                     /locus_tag="ACHKYO_RS21320"
                     /old_locus_tag="pgaptmp_004264"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HGU4431003.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAT
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         2984 a         3037 c         3320 g         3038 t
ORIGIN      
        1 ggggcacgcc gttcttgcct gtggagagat ggtgcagtca ggccgcccgt tgaagcagga
       61 acccaccgaa atgattcagg cgacagcctg aacgtagcag ggatcctcgt ccttcagggc
      121 gtggaggatg tcaatatcgg tttctgtcag cgattaaatt caagaccacg taacaaacgc
      181 ctccggcaat taatccccag aacgcagaac caatcccgac cagcgtcaat ccacttgccg
      241 ttaccagaaa tgccaccacc gccgcgtcgc gctcacgctc acgctcatta tgcagcgcct
      301 gatacaaact gccgctaatg gtacttaaca gcgccagacc tgccagcatc tggatccaac
      361 ttacgggcag ggcagccatc atcccggtaa tagcactacc aaacagacct gcgatcaaat
      421 aaaaaatgcc tgcaacggca gctgccagcc aacgttgatc tttatccgga tgcgcttccg
      481 ggctttggca aatagccgcg gtgattgccg caataccgac ggaataaacg ccgaaagggg
      541 aaaaaaccag tgccagcaat ccagtaaata caattaatgg cgaaacagga gccgaatacc
      601 cggctgcttt cattgctgcg atacccggtg cgttttgcga tgccatcgtc accagaaaaa
      661 gggggagtgc aacgctcagg ctgtgagcaa acgaaaaatc aggggtaata taagtgggga
      721 gaacgggttt aaagacaaca tcagttgtga caacgtcacc ttgcgcgata acgatcacga
      781 tcccaataat catcgcggca attaccgcat agcgcggcgc aacggccttg gttgccagcc
      841 ataccagcaa catacttcca cacaacgtaa attgaccgtc cagactggca aacgcctgta
      901 aaccaaagcg taataaaatc ccggcaagca ttgccgccgc aagcgagtgc ggaataatgc
      961 gcatcagacg agcaaagagt cccgttatgc cgcagaggac tattaacgcg ttggtgacaa
     1021 taaaaacgcc gatggcttcg ttaagtgtta gtccctgcaa tccggtgacc aacaaagccg
     1081 cgccaggcgt tgaccatgcg gtgagaacag gtacgcgata ccataatgtc agagtcagcg
     1141 tactgacgcc cattgccagc cccagcgccg tcatccagcc agagatttgt gcagtggtgg
     1201 ctccggcgac aatcgctgct tgccagatta ttgccgctga actggcgtag ccaattaata
     1261 ccgccagaaa ccccgccagt agcgtgggtg gaggaataga aaacagacgc atagtcacct
     1321 cgtgcgttat agcgtacagg caccgtagca tttgtccgtt ataacgcaca agtgataaac
     1381 ttccgttttg ccggaggagt cgcatggaaa atctcgctcg ctttttatcc accacactta
     1441 aacaactacg ccagcagcgc ggctggagtc tttcgcgact ggcagaagcg acgggcgttt
     1501 ctaaagcaat gttagggcag attgagcgta atgagtccag tccgacggtc gcgacattat
     1561 ggaaaattgc caccgggttg aacgtaccat tttcaacatt tatttctccg ccgcagtccg
     1621 ctacgccttc agtttatgat ccacaacagc aggcgatggt gatcacttcg ctgtttcctt
     1681 atgatccaca actctgcttt gaacacttct cgattcagat ggcatctggt gcaataagcg
     1741 aatcgacgcc ccatgagaag ggggtaatcg aacatgtcgt cgtcattgat ggacaacttg
     1801 atctgtgcgt tgatggggaa tggcagaccc ttaattgtgg ggaaggcgtt cgatttgctg
     1861 cagacgtcac gcatatctat cgtaatggtg gggagcaaac cgtacatttt cattccctca
     1921 tccattaccc gcgcagttaa gcgggaaaac tatttcgcaa cgtcgcgctt gtggctaaaa
     1981 tagccgccat ttttcagcta ctggataaga atgtgaccgt atcttctcat cgacttgaac
     2041 tgttaagccc ggcacgcgat gccgccattg cccgcgaagc tattttgcac ggtgccgatg
     2101 ctgtttatat cggcggccct ggttttggtg cccgtcataa tgccagtaat agcctgaaag
     2161 atattgccga gctggtgccg tttgcccatc gttatggtgc aaaaattttc gtcacgctta
     2221 acaccatttt gcatgatgat gagctggaac ccgcgcaacg gctgattact gacctctacc
     2281 agaccggtgt cgatgcgctg attgttcagg atatggggat tctggaactt gatattccgc
     2341 cgattgaact gcacgccagt acgcagtgcg acattcgtac agttgaaaaa gcgaagttcc
     2401 tctctgatgt tggcttcacg cagattgtgc tggcgcgaga gctgaatctt gatcagatcc
     2461 gcgcgattca ccaggctacg gacgcgacca ttgaattctt tattcatggg gcactgtgcg
     2521 tggcctattc gggtcagtgc tacatttctc atgcgcaaac agggcgtagc gccaaccgtg
     2581 gcgattgctc gcaggcgtgc cgtttgccat acacattgaa agacgatcag gggcgggtgg
     2641 tttcctatga aaaacatctg ctgtcgatga aagataacga tcagactgcc aacctcggcg
     2701 cgctgattga tgctggtgta cgctccttca agattgaagg gcgttacaaa gatatgagct
     2761 acgtgaagaa tatcaccgcc cattatcgcc agatgcttga tgccattatt gaagaacgtg
     2821 gcgatctggc gcgcgcttca tcaggtcgta ctgaacattt ctttgttcca tcgacggaaa
     2881 agactttcca ccgtggtagc acagattatt ttgtgaatgc ccgtaaaggc gatattggcg
     2941 cgttcgattc gccgaaattt atcggcctgc cggtaggcga agtattgaaa gtggcgaaag
     3001 atcatctcga tgttgccgtt accgagccac tggcaaatgg cgatggcctg aacgtgttga
     3061 ttaaacgtga agtcgtcggt tttcgtgcca atacggtcga gaaaaccgga gaaaatcagt
     3121 accgcgtctg gcccaatgaa atgccagcag atttgcacaa aattcgtcca catcacccac
     3181 taaaccgtaa tcttgatcat aactggcagc aggcactgac aaaaacctcc agcgaacgtc
     3241 gggtggcggt agacattgaa ctgggcggct ggcaggaaca actgattctg accctcacca
     3301 gtgaagaggg tgtcagcatc acgcatacgc tggacgggca gttcgacgaa gccaataacg
     3361 ccgaaaaagc aatgaacaat ctgaaggatg gtctggcaaa actggggcaa accctctatt
     3421 acgcccgcga tgtgcaaatt aatttgccgg gggcgctgtt tgtaccaaac agtctgttaa
     3481 accagttccg ccgtgaagct gctgacatgc tggatgctgc gcgtcttgcc agttaccagc
     3541 gcggcagccg taaaccggtt gctgatcctg cgccggttta tccgcaaacg catctgagtt
     3601 tcctcgcgaa cgtatacaac cagaaagcgc gtgaatttta tcatcgctat ggtgtgcagc
     3661 tgattgacgc ggcgtatgaa gcacatgaag agaagggcga agtcccggtg atgatcacca
     3721 agcattgtct gcgctttgcc tttaatctgt gcccgaaaca ggcgaaaggc aatatcaaaa
     3781 gctggaaggc gacgccaatg caactggtta acggcgatga agtattaacg ctaaagtttg
     3841 attgccgccc atgcgagatg cacgtcattg gcaaaatcaa aaatcacata ctgaaaatgc
     3901 cgttaccggg aagcgtagtg gcatccgtaa gtccggatga gctgctgaaa acattgttta
     3961 agcgaaaagg gtaaaacgcc agttttctgg ttactcacaa ctcattgaat ctgcatgata
     4021 ttgcctgccg ggtaaggcgt ctcgccgcat ccggcaacaa atgactcaac gttgtcccgc
     4081 ttctggtttg cgcgattttt gccagtaaaa atgctcgcgc aaaccttccg ccgactcttc
     4141 cgccacagca cgcaattcat cgctgtccgc ttcatgacgc agctgatgat ccacattctt
     4201 tacccacaca aattcatgtc ctttgtgccc tgccatgagt tgtcctgaga acagcgcaca
     4261 cgttaataag acaaccgata acgccttcgt aaacatcctg ccaccttttt cttacctttt
     4321 gccgctatga tgccgatcgt ttcttgaggt tattattcag ttttgcaaat tagcgcaaag
     4381 aaattctgga atcttccttc ctgattttgc atggcattct gccgttgcgg cgatttagtg
     4441 ctattttcga gcaaattaca cacggaggta aacaataatg tttactttgt tgatatactc
     4501 agcggcaggg aggcgatgtg aaacaaagcg agttcagacg ttggctcgaa tctcagggcg
     4561 tcgatgtagc gaatggcagc aaccatttga aactcaggtt tcatgggagg cgcagtgtca
     4621 tgccgcgtca cccctgcgat gagattaaag aaccattgcg taaagcaatc ctgaaacaac
     4681 tcggtttgag ttaatcgcca attaaaaagg ttaatgacat gcgagggaca gtcgaaatta
     4741 tgcgttatcc cgtcactctt acacccgcgc cggaaggcgg ttatatggtt tcttttgtgg
     4801 atatccctga agcgttgacg cagggcgaaa ctgtcgctga agcgatggaa gcggcaaaag
     4861 atgctttact gaccgcattt gatttttatt ttgaagataa cgagcttatc cctttacctt
     4921 cgccattaaa tagtcatgat cactttattg aagtaccttt gagcgtcgcc tctaaggtat
     4981 tgctgttaaa tgctttttta cagtcagaaa tcactcagca agagttagcc aggcgaattg
     5041 gcaaacctaa acaggagatt actcgcctat ttaacttgca tcatgcgaca aaaatcgacg
     5101 ccgtccagct cgcggcaaag gcgcttggca aagagttatc gctggtgatg gtttaattac
     5161 agttaacgaa aagttgtcat ttttaacaac tgatatagac tgccgaatca tctgcacata
     5221 attacgattc gataatgaaa aaataccagc agcttgcaga acaattacgc gagcagattg
     5281 cgtcgggtat ctggcaaccc ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt
     5341 ctggcatgag ctttatgact gtcagccatg cctatcagtt gctcgaaagt ctgggatata
     5401 ttatcgcgcg accgcagtcg ggttattacg ttgcgccaca ggcaataaaa atgccgaaag
     5461 cgccagtcat tccagtcact cgagatgaag cagtcgatat caacacttat atttttgata
     5521 tgttgcaggc cagtcgcgat ccgtcggtcg ttccgtttgc ctcggccttt cccgacccgc
     5581 gacttttccc cctccaacaa ctaaaccgct cgctggcgca ggtaagcaaa accgccacag
     5641 cgatgagcgt gattgaaaac ttaccgccag gaaacgcaga actgcgtcag gctattgctc
     5701 gtcgctatgc cttacagggc atcaccattt ctcctgatga aattgtcatt actgccgggg
     5761 cgttagaggc attaaacctc agtttgcaag cggtaactga accgggcgat tgggtgatag
     5821 tagagaatcc ttgtttctac ggtgcgttgc aggcgctgga gcggctacgg ctgaaggcgt
     5881 tatcggtggc gacggatgtt aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc
     5941 aggattatcc ggtgaaagcg tgctggctga tgactaatag ccagaaccca ctcggattta
     6001 ccttaacgcc gcaaaaaaaa gcacaactgg tggcgttact caatcagtac aacgtaacgc
     6061 tgattgaaga tgacgtttac agcgaacttt attttggacg ggaaaaaccg ctgcctgcga
     6121 aagcgtggga tcgccacgat ggcgttttgc attgctcttc gttttcgaaa tttctggtgc
     6181 ctggttttcg tattggttgg gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc
     6241 agttgatgag tacgctttcc accagctcac cgatgcaact tgcgctggtg gattaccttt
     6301 ccacgcgccg atacgacgcc catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac
     6361 aacgtgcctg gcaggcactg ctgcgttatc tgcctgcgga agttaaaatt catcatagtg
     6421 acagcggtta ttttctctgg ctggagctcc ccgagccgtt agatgccggc gaattaagcc
     6481 tggcggcact gacgcatcat atcagtattg cgccgggtaa aatgttttct accggtgaaa
     6541 actggtcacg tttttttcgt tttaataccg cgtggcagtg gggagaacgt gaagaacagg
     6601 cggtaaaaca attaggcaaa cttattcaag aacggctgta atagcgttta atttaattcc
     6661 tcttagattg ggtaatatga atttcgaata gcagtcatat ttcctaaccc cttgtctata
     6721 ctccagaaga taaccttaca gacggcataa tgcgcggtag ctcacaacct gattaaattt
     6781 tctcaggggc gaaggtgtgc ctgcaagccg ccgtctatgg ttaaacaagg agatattttt
     6841 acggcacggc ggctgaacaa ttaattacga caggagtaag accttatgag caagacattt
     6901 gcccgcagca gcctgtgtgc gctcaccatg acaataatga ccgctcacgc cgccgaacca
     6961 cctaccaatt tagataaacc ggaagggcga ctggatatta tcgcctggcc gggatacatc
     7021 gaacgcggac aaactgataa acaatacgac tgggtaacgc aatttgaaaa agagacaggc
     7081 tgcgcggtga atgtgaaaac cgccgcgact tccgatgaaa tggtcagtct gatgaccaaa
     7141 gggggttacg atctggttac ggcatccggc gatgcctcgc tgcgtttgat tatgggcaaa
     7201 cgcgtgcagc cgattaatac cgccttgatt cccaactgga aaacgctcga tccgcgcgtg
     7261 gttaaaggcg actggtttaa tgttggcggc aaagtttacg gcacacctta ccaatggggg
     7321 ccgaacctgc tgatgtacaa cactaaaacc ttcccgacgc cgccggatag ctggcaagtg
     7381 gtttttgttg agcaaaatct gccggacggc aagagcaata aaggccgcgt tcaggcttat
     7441 gatggcccta tctacattgc ggacgctgcg ttgttcgtta aagccactca gccgcagttg
     7501 ggcatcagcg atccgtatca actaaccgaa gaacagtacc aggcggtgct gaaagtgctg
     7561 cgcgctcaac acagtttgat ccatcgctac tggcatgaca ctaccgtgca aatgagcgat
     7621 ttcaaaaacg agggtgtggt tgcttccagt gcctggccct atcaggccaa cgccctgaaa
     7681 gccgaaggcc agcctgttgc taccgttttc ccgaaggagg gtgttaccgg ttgggctgat
     7741 accaccatgc tgcatagcga agcgaaacat ccggtttgcg cctacaaatg gatgaactgg
     7801 tcattaacgc caaaagtgca gggcgatgtg gcggcctggt ttggctcgtt accggtagtg
     7861 ccggaagggt gtaaagccag tccgttatta ggcgaaaaag gttgtgaaac caacggtttt
     7921 aactatttcg acaaaatcgc cttctggaaa acgcctatag cagaaggggg caagtttgtt
     7981 ccctacagtc gctggacgca ggattacatt gccattatgg gcggtcgcta acttcgctgg
     8041 ggtgctttat gacgtacgca gtggagtttg acaacgtctc gcggttgtac ggtgacgtac
     8101 gcgcagtaga tggcgtcagt attgcgataa aagatggtga gttcttctct atgctggggc
     8161 cgtccggctc cggcaaaacc acctgcctgc gcctgattgc tggcttcgaa cagctttccg
     8221 gcggggctat ctctatcttt ggtaaacccg ccagcaatct gccaccgtgg gagcgggacg
     8281 tgaatactgt ctttcaggac tacgcgctat ttccgcatat gtcgattctt gacaatgtcg
     8341 cctatgggct gatggtcaaa ggcgtgaata aaaagcagcg gcacgcaatg gcgcaagagg
     8401 cgctggagaa agtggcgttg gggtttgtac atcaacgtaa accgtcacaa ctttctggtg
     8461 gtcagcgcca gcgggttgct atcgccagag cattggtgaa tgaaccgcgc gtattgctgt
     8521 tggatgaacc gctcggcgca ctggatctca aattgcgtga gcagatgcag ctggaactga
     8581 aaaaactgca acagtctctc ggtatcactt ttatcttcgt tacccacgat cagggcgaag
     8641 cgttatcgat gtccgatcgt gtggcggttt tcaataatgg acgcattgag caggtcgatt
     8701 ccccgcgcga tctctatatg cgcccgcgca cgccgtttgt tgccggattc gtcgggacat
     8761 ccaacgtttt tgatggactg atggcagaga aactttgtgg catgacggga agcttcgccc
     8821 tgcgaccgga acatatccgc ctcaacaccc ctggtgaact gcaggccaat ggcacgatcc
     8881 aggcggtgca atatcagggc gcggcaactc gttttgaact gaaattgaac ggcggtgaaa
     8941 aactgcttgt gagtcaggcc aatatgacag gcgaagaact gcctgccacg ctcacgcccg
     9001 gacaacaggt gatggtttcc tggtcgcgtg atgtgatggt gccgctggtt gaggagaggt
     9061 gaatggcgat gaatgtattg caatcacctt cacgtccagg tctgggtaag gtgtccggtt
     9121 tcttctggcg taatccgggg ctggggctgt ttttactgct gcttggcccg ctaatgtggt
     9181 ttggcattgt ctatttcggc tcgctgctga cactgttatg gcagggattt tatacttttg
     9241 acgatttcac catgtcggta acgccggaac tgacgctggc gaatatccgt gcgctgttta
     9301 atccggcgaa ttacgacatc attctccgca cgctgactat ggctgtggca gtcactatcg
     9361 ccagcgccat tctggctttt ccaatggcgt ggtatatggc gcgctatacc agcgggaaaa
     9421 tgaaagcttt tttttatatt gcggtaatgt tgccgatgtg ggcgagctac attgttaaag
     9481 cctatgcctg gacgttattg ctggcaaaag atggcgtggc tcagtggttt ttacaacatc
     9541 ttgggctgga accactgctg ctgcgttcct tacattacct gcggtgggcg gaaatacgct
     9601 gtcaacttcc gggctggggc gctttctggt gtttctctat atctggttgc cgttcatgat
     9661 cctgcccgtt caggcggcgc ttgagcgttt gccgccgtca ttgttgcagg cgtcggctga
     9721 tctcggcgca cgtccacgac aaacctttcg ctatgtggtg ctgccgctgg caatcccggg
     9781 tattgccgct ggctctatct ttaccttctc actcacactg ggcgatttta tcgtcccgca
     9841 gctggttggt cctccaggat attttatcgg caatatggtt tattcccagc agggggcgat
     9901 tggcaatatg ccgatggcgg cggcattcac cctggtgccg attgttctca tcgcactgta
     9961 cctggcgttc gtgaaacgtc tgggagcgtt cgatgcactc tgaacgcgca ccgtttttcc
    10021 tcaaactggc ggcctggggc ggcgtggttt tcctacattt tcccatcctg attatcgccg
    10081 cttatgcgtt taacactgaa gatgcggcgt ttagttttcc accgcagggc ctgacgctgc
    10141 gctggtttag cgtggcagca cagcgtagtg atattcttga tgccgtgaca ctgtcactta
    10201 aagtggcggc gctggcgaca ttaatggcgc tggtgttagg gacgctggca gctgccgcgc
    10261 tgtggcgacg agactttttc ggcaaaaacg ccatttcgct gttactgctg ctgcccattg
    10321 cgctgccggg cattgtcact ggtctggcgc tattaaccgc ttttaaaacc atcaatctgg
    10381 agccgggatt tttcaccatc gtggtcggtc atgcgacttt ttgcgtagtg gtggtgttta
    10441 acaatgtcat cgcccgtttt cgccgcacct cgtggagtct ggttgaggcg tcaatggatc
    10501 ttggggccaa tggctggcaa accttccgct acgtagtgtt gccgaatctc agttcggcgc
    10561 tactggcagg aggaatgctg gcgtttgcct tgtcgttcga tgaaatcatc gttacgactt
    10621 ttacggcagg tcatgaacga acgttaccgt tgtggttgct caatcagctt gggcgaccgc
    10681 gtgatgtacc ggtaactaac gtggtggcac tgctggttat gttggtaaca accttgccga
    10741 tcctgggggc ctggtggcta acccgcgaag gcgacaatgg tcaataacca ctgatacagg
    10801 aatatgctat gcaacataag ttactgatta acggagaact ggttagcggc gaaggggaaa
    10861 aacagcctgt ctataatccg gcaacggggg acgttttact ggagattgcc gaggcatccg
    10921 cagagcaggt cgatgctact gtgcgcgcgg cagatgcagc atttgccgaa tgggggcaaa
    10981 ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc tgatgttatc gaagaaaatg
    11041 gtcaggtttt tgccgaactg gagtcccgta attgtggcaa accgctgcat agtgcgttca
    11101 atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt cgcgggtgcg gcgcgctgtc
    11161 tgaatggtct ggcggcaggt gaatatcttg aaggtcatac ttcgatgatc cgtcgcgatc
    11221 cgttgggggt cgtggcttct atcgcaccgt ggaattatcc gctgatgatg gcagcgtgga
    11281 aactggctcc ggcgctggcg gcagggaact gcgtagtgct taaaccatca gaaattaccc
    11341 cgctgaccgc gttgaagttg gcagagctgg cgaaagatat cttcccagca ggcgtgatta
    11401 acgtactgtt tggcagaggc aaaacggtgg gtgatccgct gaccggtcat cccaaagtgc
    11461 ggatggtgtc gctgacgggc tctatcgcca ccggcgagca catcatcagc cataccgcgt
    11521 cgtccattaa gcgtactcat atggaacttg gtggcaaagc gccagtgatt gtttttgatg
    11581 atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt tggctattac aatgctggac
    11641 aggattgtac tgcggcttgt cggatctacg cgcaaaaagg catttacgat acgctggtgg
    11701 aaaaactggg tgctgcggtg gcaacgttaa aatctggtgc gccggatgac gagtctacgg
    11761 agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt cagcaaggca gtagaagagg
    11821 cgaaagcgac agggcacatc aaagtgatca ctggcggtga aaagcgcaag ggtaatggct
    11881 attactatgc gccgacgctg ctggctggcg cattacagga cgatgccatc gtgcaaaaag
    11941 aggtatttgg tccagtagtg agtgttacgc ccttcgacaa cgaagaacag gtggtgaact
    12001 gggcgaatga cagccagtac ggacttgcat cttcggtatg gacgaaagat gtgggcaggg
    12061 cgcatcgcgt cagcgcacgg ctgcaatatg gttgtacctg ggtcaatacc catttcatgc
    12121 tggtaagtga aatgccgcac ggtgggcaga aactttctgg ttacggcaag gatatgtcac
    12181 tttatgggct ggaggattac accgtcgtcc gccacgtcat ggttaaacat taagttagat
    12241 gttagtggct gttataggac ggccactatc gttttaggtt ctgagtttaa tggcggttaa
    12301 gtattagaat gattacatta ataactagcg taaaaattag gctaaatgtt tgatggcgat
    12361 ttttcatcct gtcacagtt
//
LOCUS       DAZGAN020000137        12201 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGAN020000137 DAZGAN020000000
VERSION     DAZGAN020000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.137"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            33..551
                     /locus_tag="ACHKYO_RS21325"
                     /old_locus_tag="pgaptmp_004265"
     CDS             33..551
                     /locus_tag="ACHKYO_RS21325"
                     /old_locus_tag="pgaptmp_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HGU4431004.1"
                     /translation="MNINSDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(596..2641)
                     /gene="dcp"
                     /locus_tag="ACHKYO_RS21330"
                     /old_locus_tag="pgaptmp_004266"
     CDS             complement(596..2641)
                     /gene="dcp"
                     /locus_tag="ACHKYO_RS21330"
                     /old_locus_tag="pgaptmp_004266"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HGU4431005.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     SYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            2778..3524
                     /gene="ydfG"
                     /locus_tag="ACHKYO_RS21335"
                     /old_locus_tag="pgaptmp_004267"
     CDS             2778..3524
                     /gene="ydfG"
                     /locus_tag="ACHKYO_RS21335"
                     /old_locus_tag="pgaptmp_004267"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HGU4431006.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3613..4299
                     /gene="rspR"
                     /locus_tag="ACHKYO_RS21340"
                     /old_locus_tag="pgaptmp_004268"
                     /gene_synonym="ydfH"
     CDS             3613..4299
                     /gene="rspR"
                     /locus_tag="ACHKYO_RS21340"
                     /old_locus_tag="pgaptmp_004268"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HGU4431007.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4477..4680
                     /gene="ydfZ"
                     /locus_tag="ACHKYO_RS21345"
                     /old_locus_tag="pgaptmp_004269"
     CDS             4477..4680
                     /gene="ydfZ"
                     /locus_tag="ACHKYO_RS21345"
                     /old_locus_tag="pgaptmp_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HGU4431008.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4716..6176)
                     /locus_tag="ACHKYO_RS21350"
                     /old_locus_tag="pgaptmp_004270"
     CDS             complement(4716..6176)
                     /locus_tag="ACHKYO_RS21350"
                     /old_locus_tag="pgaptmp_004270"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU4431009.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(6265..7548)
                     /gene="ydfJ"
                     /locus_tag="ACHKYO_RS21355"
                     /old_locus_tag="pgaptmp_004271"
                     /pseudo
     CDS             complement(6265..7548)
                     /gene="ydfJ"
                     /locus_tag="ACHKYO_RS21355"
                     /old_locus_tag="pgaptmp_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            8152..8265
                     /locus_tag="ACHKYO_RS21360"
                     /old_locus_tag="pgaptmp_004272"
     CDS             8152..8265
                     /locus_tag="ACHKYO_RS21360"
                     /old_locus_tag="pgaptmp_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HGU4431010.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            8334..8567
                     /gene="ydfK"
                     /locus_tag="ACHKYO_RS21365"
                     /old_locus_tag="pgaptmp_004273"
     CDS             8334..8567
                     /gene="ydfK"
                     /locus_tag="ACHKYO_RS21365"
                     /old_locus_tag="pgaptmp_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HGU4431011.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKM"
     gene            8884..9474
                     /locus_tag="ACHKYO_RS21370"
                     /old_locus_tag="pgaptmp_004274"
     CDS             8884..9474
                     /locus_tag="ACHKYO_RS21370"
                     /old_locus_tag="pgaptmp_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4431012.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
     gene            complement(9572..10147)
                     /locus_tag="ACHKYO_RS21375"
                     /old_locus_tag="pgaptmp_004275"
     CDS             complement(9572..10147)
                     /locus_tag="ACHKYO_RS21375"
                     /old_locus_tag="pgaptmp_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4431013.1"
                     /translation="MAFRMSEQPQTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEAWKKYRVLLNRVDTSTAQDIEWPALP"
     gene            complement(10147..>12201)
                     /locus_tag="ACHKYO_RS21380"
                     /old_locus_tag="pgaptmp_004276"
     CDS             complement(10147..>12201)
                     /locus_tag="ACHKYO_RS21380"
                     /old_locus_tag="pgaptmp_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431014.1"
                     /translation="SSASAASTSAGQASASATAAGKSAESAASSASTATTKAGEATEQ
                     ASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASSASASKDEATRQAS
                     AAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVALEDAST
                     TKKGIVQLSSATNSTSESLAATPKAVKAAYELANGKYTAQDATTAQKGIVQLSNATNS
                     TSEMLAATPKSVKAAYDLANGKYTAQDATTTQKGIVQLSSATNSASETLAATPKAVKA
                     ANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGAGWFKLATVTMPQA
                     SSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQRTSTGFTNFAWVN
                     TSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPEGLTDGTVYSLYTP
                     SEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGK
                     PASGRAVLSQEQDGIKSHTHSASASSTDLGTKNTSSFDYGTKSTNNTGAHTHSISGTA
                     NSAGAHQHKSSGAFGGTNTSIFPNGYTAISNLSAGIMSTTSGSGQTRNAGKTSSDGAH
                     THSLSGTAASAGAHAHTVSIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVR
                     LA"
BASE COUNT         3181 a         3061 c         2778 g         3181 t
ORIGIN      
        1 acctttacat ataaaagcgt ggacaaaaaa cagtgaacat taatagcgat aaaattgtac
       61 aacttgcaga taccgacact attgaaaacc tgacatccgc gttgagtcaa agacttatcg
      121 cggatcaatt acgcttaact accgccgaat catgcaccgg cggtaagttg gctagcgccc
      181 tgtgtgcagc tgaagataca cccaaatttt acggtgcagg ctttgttact ttcaccgatc
      241 aggcaaagat gaaaatcctc agcgtaagcc agcaatctct tgaacgatat tctgcggtga
      301 gtgagaaagt ggcagcagaa atggcaaccg gtgccataga gcgtgcggat gctgatgtca
      361 gtattgccat taccggctac ggcggaccgg agggcggtga agatggtacg ccagcgggta
      421 ccgtctggtt cgcgtggcat attaaaggcc agacctatac tgcggttatg cattttgctg
      481 gcgactgcga aacggtatta gctttagcgg tgaggtttgc cctcgcccag ctgctgcaat
      541 tactgctata accaggctgg cctggcgata tctccaggac agccattggt ggtgtttata
      601 tgttcaagcc acgatgttgc agcatcggca taatctgagg agccttaccg cgccattgtc
      661 gatacaggcg ttccagatct tcgctgttac ctctggaaag gatcgcctcg cgaaaacgca
      721 gcccattttc acgcgttaat ccgccctgct caacaaacca ctgataacta tcatcggcca
      781 acatttgtgt ccacagataa gcgtaataac ctgcggcata tccgccacca aaaatatggg
      841 cgaaataact gctgcgatag cgtggcggta tagcaggaag atccatattt tccgccacca
      901 gcgcccgcaa ttcaaaatca tcgacatcct gcattgcttc gttttcttct aggcaatgcc
      961 agcgcatatc gagaagtgcg gcgctaagca gttcgctcat ctcataccct ttgttgaaca
     1021 ggctggcatt acgcattttc tgttgcagtt cgtcaggcat tgctgccccg ctctgataat
     1081 gccgggcgta gcgagcgaat acctgcggat gcgttgccca gtgttcgttg atttgcgacg
     1141 gaaattcgac aaaatcacgc ggcgtgttgg tgccggaaag cgtggcataa cgctggcggg
     1201 caaaaaggcc gtgcagcgta tgaccaaatt catggaataa ggttatgaca tcatcccaga
     1261 gtaacaacgc aggctcaccg gcagcgggtt tctgataatt gcaaacgtta taaattaccg
     1321 gatgcgtttc attaagcgtt gattgctcaa caaaattgcc catccatgca ccgccgcttt
     1381 ttgaatcacg ggcgaagaaa tcaccgtaaa ataacgccag tcccacgcca ttatgatcaa
     1441 aaatttccca cacacgaacg tcaggatggt agacaggaat atcaaaacgt tcgacaaact
     1501 taataccgaa gagctgattc gcggtccaga atacaccttc attcaacacc gtgtttaatt
     1561 caaaatatgg cttgagctgc gcctcatcaa gatcaaattt ctcccgccgt acctgttcgg
     1621 cataaaatgc ccagtcccac ggctgcgcgc taaacccgcc ttgctgctta tcgataaccg
     1681 cctgtatgga ggctaattca tcgctagcac gttgacgcgc cgctggaaca atttcccgca
     1741 taaagttgag tgctgcttca ggtgtttttg ccatctgatc ggcgattttc catgcggcat
     1801 aatgaggaaa accaagcagt ttcgcctgct gtgcgcgaat ctctaccaga cgttgaatga
     1861 tagcgcgggt atcattgcca tcattttttt ccgctcgcgt ccagcccgca ataaacagtt
     1921 tttcacgcgt cgcacgatcg cgcatttcgg caagcgccgg ttgttgggtg gtattcagca
     1981 gcggaatcag ccatttgtta tccagacctt tctcgcgagc cgcctctgcc gccagcgcaa
     2041 tctcttgctc actcattcct gccagctgcg cgatatcgtt cacaaccaga ccgccggatt
     2101 tatttgctgc aagtaaccgc tggttaaact ggctggtcag ggtcgcagct tctgtattca
     2161 gtacttttaa ttttgcttta tcagcttgcg caagtttggc tccggcaagg acaaaacgtt
     2221 gatgaataac ctccaccagg cggatggatt cactatcaag ccccagggat tcacggcgct
     2281 gccagacagc atctacccgc gcgaataatt caccgttcag atagatatca ttagccagtt
     2341 ccgccagttc agcggaaaac tgttcatcaa gacgctgtaa ttcatcattg gtatgcgccg
     2401 cagtcatcgc aaaaaagacg ctggtaacgc gggtaagtaa ttctccgctt tgttccagtg
     2461 ccagaatagt attgttgaaa tcaggcgttt gcgggttaag cgcgatggca gcaatttctg
     2521 cccgcttttg ctgcattccc tcatcgaatg ccgggcgata gtgatgattg gcaatttgat
     2581 caaaatgagg agccagatac ggcagtgtgc tttgcacaag gaaaggattc attgttgtca
     2641 ttttcttctc ctgaacgcga ggtgttccat agcgtaggct tactgataac ggagtgcaat
     2701 cttgcaaacc agtattaccc gctcttaagc atcacgtgct atgttattga cacacaaaag
     2761 cgttgaggaa cagtgagatg atcgttttag taactggagc aacggcaggt tttggtgaat
     2821 gcattactcg tcgttttatt caacaagggc ataaagttat cgccactggc cgtcgccagg
     2881 agcggttgca ggagttaaaa gacgaactgg gagataatct gtatatcgcc caactggacg
     2941 ttcgcaaccg cgccgctatt gaagagatgc tggcatcgct tcctgccgag tggtgcaata
     3001 ttgatatcct ggtaaataat gccggcttgg cgttgggcat ggagcctgcg cataaagcca
     3061 gcgttgaaga ctgggaaacg atgattgata ccaacaacaa aggcctggta tatatgacgc
     3121 gcgccgtctt accgggtatg gttgaacgta atcatggtca tattattaac attggctcaa
     3181 cggcaggtag ctggccgtat gccggtggta acgtttacgg tgcgacgaaa gcgtttgttc
     3241 gtcagtttag cctgaatctg cgtacggatc tgcatggtac ggcggtgcgc gtcaccgaca
     3301 tcgaaccggg tctggtgggt ggcaccgagt tttccaatgt ccgctttaaa ggcgatgacg
     3361 gtaaagcgga aaaaacctat caaaataccg ttgcattgac gccagaagat gtcagcgaag
     3421 ccgtctggtg ggtgtcaacg ctgcctgctc acgtcaatat caataccctg gaaatgatgc
     3481 cggttaccca aagctatgcc ggactgaatg tccaccgtca gtaattttta tacccggcgt
     3541 aactgccggg ttattgcttg tcacaaaaaa gtggtagact catgcagtta actcactcac
     3601 aagcaagaac gaatgaccgt cgaaacgcaa cttaatccca cacagcctgt caatcagcag
     3661 atttatcgta ttcttcgtcg cgacattgtc cattgcctga ttgcaccagg cacaccgttg
     3721 tcggaaaaag aagtttctgt tcgtttcaat gtgtcacgcc agccggttcg tgaagccttt
     3781 attaaactgg cggaaaacgg cctgattcaa attcgtccgc agcgtggcag ctacgtcaac
     3841 aaaatttcca tggcccaggt gcgaaacggc agttttatcc gtcaggccat tgagtgcgcg
     3901 gtggcgcgtc gggcggcgag catgattacc gaaagccagt gctaccaact ggaacaaaat
     3961 cttcaccagc aacgcattgc cattgagcgc aagcaactgg atgatttttt tgaacttgat
     4021 gacaacttcc atcaactcct gacgcagatt gccgactgtc aactggcgtg ggataccatt
     4081 gagaacctga aagcgaccgt tgatcgcgtg cgctatatga gtttcgacca tgtttctcca
     4141 ccagaaatgc tgttacgcca gcatcttgat attttctctg ccctgcaaaa acgtgatggc
     4201 gatgcggtag aacgtgcaat gacgcaacat ttgcaggaaa tcagcgaatc cgtgcgccag
     4261 atccgccagg aaaacagcga ctggtttagc gaagagtaat tcatttactc tcatcccatc
     4321 cgggatgaga ggcttttccc cccgccttac ggctcatgca tgcatcaaaa aagatgtgag
     4381 cttgatcaaa aacaaaaaat atttcactcg acaggagtat ttatattgcg cccgttacgt
     4441 gggcttcgac tgtaaatcag aaaggagaaa acacctatga cgacctacga tcgtaaccgt
     4501 aacgcaatca ccactggcag ccgtgttatg gttagcggca ccggtcacac tggcaagatc
     4561 ctgtcgattg atactgaagg tctgaccgct gagcaaatcc gccgcggaaa aaccgtagtt
     4621 gttgaaggtt gtgaagagaa actggcacct ctggacctga ttcgtctcgg catgaactaa
     4681 gtgtgtgaat gccgccgatg gcggcattgc tttttttact tcacggaata tttcgccacg
     4741 gtcgctttcg cgccatgcgc taataaagac aagtacgctt ccgtcacttt tgcagtaaac
     4801 aagctattgt ctggcaaatc atcaccaaag attgccttaa tcgccagcaa tgactggacg
     4861 cgcgctttcc cttcggcact actttgtaca gccttctgaa taacaggtaa cagtgggtca
     4921 ctgatttcta tcggatttcc ctgttcatca acaccaccga cataacgcat ccaacccgcg
     4981 acgcccagcg ccagcagatc gaacttgctg tcatgcgcca gatgccagcg aacagaatcc
     5041 aacatccgct gtggcaattt ctggctacca tccatcgcaa tctgccaggt tcgatgacgt
     5101 aacgccggat tgctatagcg ttcaattaat cggttagcgt aatcttgcaa atcaacgccc
     5161 tgcactttca gcgtcggcgc ttgttcctgc aacatcaagc catacgccgc ataacgataa
     5221 tgttcatctt ccatgcagtc attaatgtgc tgatatccgg caagataacc caaatacgcc
     5281 aggaatgaat gactgccgtt gagcatgcgc aacttcatct cttcataagg cagcacatca
     5341 ctaaccagtt cggctcccgc tttttcccat tctggacgtc cggcaacaaa gttatcttct
     5401 attacccact ggcggaaagg ttcacaggca acgcccgcag gatcgcccac accggtaagt
     5461 tgttcgattt tcgccagcgt atcctctgtc actgcgggca caatacggtc caccattgtt
     5521 gatgggaaag tcacgttatc ttcgatccat tgtgccagtt ttacatcaac agctttcgcg
     5581 tatgaagtga caacgtcacg cataacatga ccgttttctg gcatgttgtc acatgacatg
     5641 acggtaaatg cgggaagtcc tgccgcttta cggcgggcca gcgcctcaac aatcacccct
     5701 gttgctgttt tcggctggtg gggattttgc acgtcggcag cgaccatcgg gtgatcgagc
     5761 attaactgtc cggtcgccgg agagtggaaa tacccttttt cggtgattgt cagagagaca
     5821 atcgcgattt gcggttcaca catcgcagcc aacacggttt ctaagccatc catctgtacg
     5881 tgcaaggctt ttttaacgac gccaacgacg cgagccgtcc acgcatcggc cgacatttcc
     5941 gcaacggtat aaagattatc ttgctgttgt aaatcggcaa tttgctgttc gccgccgatt
     6001 aagttgacct cataatatcc ccagtcactg aaatgttctg tagcaagaat atcggcatac
     6061 acgccctgat gcgcacggtg aaatgcacca aagcctaaat gaataattct tggggccagg
     6121 ttattacgat cataaacagg gagtgtcgct tttgctgata acaaattatt tcccataaca
     6181 attccttaaa tttaaatatg gcaagctata tgttttgtta tatgaataaa aatcccctct
     6241 ccggtaagag aagggattga gggtttacag acttctggaa ggttgcgcag ctcttacaac
     6301 acgcggttga tcttccgcag cgtcttccag cgcacttaaa tcacggtctt tcacctctgg
     6361 cattttcagc gcagagatta aaccaattac tgaatatgcc atgatcataa tggcgatcgg
     6421 ataccaggat tccgtcatgg tgcagaaaat acccgccagg ataggaccaa aaccggaagc
     6481 gataatacca ccaatttctt tagaaatagc catccgggta aagcggtttt tacagccgaa
     6541 catttctgcc atggtaatgt tttccagagc aaataatccc agcaccgcac agttatgaat
     6601 cacaatcagt gcaaccataa tggtgctcgg ggcatagctt ttatctacaa tgatagaaag
     6661 cattggccat gccagcacaa tcgcggaggt attcataata atatacggga tccggcgacc
     6721 aattttatcg gataaccaac caaggaacgg aatggtcata aagccgagaa tcgaactaat
     6781 catcaatgca tctgttggaa ttgctttgtt aaacaataac gtctgcacta aatagcctgc
     6841 aaggaaagtc tgaattaacc cggagttacc cgcctgacca aaacgcagcc ctgttgccag
     6901 ccagaaggat ttgctctgga acatgctacc agcaggtgca ggttttgctg tgggttggtt
     6961 gctgtcatta accttctcaa agaccgggct ttctttcaga ttcatacgta accagatagc
     7021 aaagaccatc acgacaacgc tcgccaggaa cggtatacgc catccccacg ccagcagttc
     7081 ctctttactg agaatgaaga acataaaggc ccagattgcc gttgcgctca aggttccgca
     7141 gttagttccc atagctacaa atgaggagat aattccgcgc ttaccttttg gtgcatattc
     7201 cgccagcatc gtaccggcac cggaaatttc cgcacctgta cccaacccct gaataatacg
     7261 cagcgtcacc agcaagatgg gggcaaaaac accaatctgt gcataggtcg gtaacacacc
     7321 aattaaggtg gtacagatcc ccatcatggt gatggtaata aagagcactt ttttacgccc
     7381 tattctgtcg cccattttgc cgaaaataaa tgctccgaca atacgcgcca cataacctgc
     7441 accgtaggtt cccattgcca aaattaacgc cattgccgtt gatgattcag gaaaaaatat
     7501 ttcatgaaac actaacgctg cgccgagcga atataactgg aaatccataa ttcacaggtg
     7561 ttttttccca tcctgtggtt tccttggcgt tttctaggtt ttttcagata gttgcatttt
     7621 ttaaaaagca tcctaagttc gatctcagtg tctatctggg gcctatttct gtcccatata
     7681 tgccccaaaa aaactcccca acagataagt agttttttca tggatttatg cgtaaaatca
     7741 agaacggctg gaaatcattc aatactcaca ctatcgaaaa atttaccagc caaccgcagc
     7801 acgttcttgc ataaggcgtg tctgcggttt ttcaactatt cagatacatc actcccatca
     7861 cattcattcc tccgcatcaa aggcatatag gctatatcac cttgatattt ttcttcttca
     7921 gataaaaact gttatctatg tatactttta aacccaatcc gtgtagagtc tctgcataag
     7981 atagtttgca gttgccgctt cagcttgcgc cataaaccgc ctgatttttg ctgccacctg
     8041 ttagcattcc tgtatacctg aaacgacaat gtttatctac gaactttaag aacacccaag
     8101 ataaaaattg tcaactatat catatataac acattactaa ttcgaggcta tatgaacagc
     8161 atactgataa tcacttctct ccttatcata ttcagcattt ttagtcatgc cctaataaaa
     8221 ttagggattg gcatatccaa taacccagac aaaaccgatg tataagtcaa catatcctga
     8281 atcagacata caatatcgca atgaaaatca ataatatttt aaggaatatc ttcatgaaat
     8341 caaaagacac cctaaagtgg ttccctgcgc agcttcctga agtaagaatt atcctagggg
     8401 atgctgtagt ggaagtagca aaacagggaa gacctattaa taccagaaca ttgcttgatt
     8461 acattgaagg aaacataaag aaaaaatcat ggctggataa caaagaatta ttacaaacag
     8521 cgatatcagt tcttaaagac aaccaaaatt taaatggtaa aatgtaatat aataaactta
     8581 cttttttatc atttttccac ttcaacaaca ttttgctcca cttttccacg tccaaacaac
     8641 ttgaaatctg gttaaaataa cacgcaacac tattcttctt ccttgagtcc gcccggaact
     8701 cgaaaaacaa accgagttaa agccattttt cacaaaatca attttgggtc tcaccaaaat
     8761 tacggggttg catacgcatt cgtttatttt cgaacgtgta catacaaata tgcacaaaaa
     8821 taatcataat tattttctga gatgcattat gatatgaaca ccaatttcgt atagagtctc
     8881 actatgtctc gaatttttgc ttactgtcgg atatcaacgc tggatcagac caccgaaaat
     8941 caacgccggg aaatcgaaag tgcaggtttt aaaatcaaac ctcagcaaat aatcgaagaa
     9001 cacattagcg gctcagcagc aaccagtgag cgtcctggtt ttaaccggtt gcttgctcgc
     9061 ctgaaatgtg gtgatcaatt gattgtgaca aaactggatc gccttggttg taatgcaatg
     9121 gatatcagga aaacagtgga acaactgacc gaaacaggta tcagagtgca ttgcttggca
     9181 ttgggtggca ttgacctgac cagtccaaca ggaaaaatga tgatgcaagt aatttcagca
     9241 gtcgctgaat ttgaacgaga ccttttactt gaacgcactc attccgggat agtaagagcc
     9301 cgcggcgcag ggaaacgttt tggtcgacca cctgtgttaa atgaagaaca gaaacaggtg
     9361 gtattcgaac gaattaagtc aggtgtaagt ataagtgcca ttgcccggga attcaaaacc
     9421 tcgcggcaaa ccattttaag agccaaagca aaacttcaga cacctgacat ataaaaaata
     9481 atctcggtgt gagatgcttt acgtgttcca agcccccttc cttgccgtaa atggaaagat
     9541 acatctaatt atagaattta tatgttttac cctacggcag tgctggccat tcaatatcct
     9601 gtgcagttga cgtatcaaca cggttcagca atacccgata ctttttccat gcttccagca
     9661 acgagatttc ttcctccgtt gcaatttcca gatctgcagc atcctgaagc ggcgcaatat
     9721 gctcactggc tacctgcatc aggttgtttt ttgtttcttc cgcctcccgg atccggaaca
     9781 gtttttctgc ttccgtatcc ttcacccaag ctgtgccgtt ccacttctga aactcccctt
     9841 ccggcgataa ccaggtaaca ttttccggta acggaccgag ttcagaaata aataacgcgt
     9901 cgccggaagc cacgtcatag acggtttttc cccgatgatc ttcaacgaga tgccacgatg
     9961 cctcatcact gttgaaaaca gccacgaagc ctgccggaat atctggcggt gcaatatcgg
    10021 tactgtttgc tggcagacct gtatgaggcg gaatatatgc gtcaccttca ccaataaatt
    10081 cattagttcc ggccagcaga ttataaattt ttatggtctg tggttgttca ctcattctga
    10141 atgccattat gcaagcctca caatatagtt aaatgcgatg tttttgacgg tgttttccgc
    10201 gttaccagca gcgttaacgg tgatggtgtg tccatgtgaa ccaatcgcaa cggagtgcgt
    10261 atgagcacca atactgacag tatgtgcatg tgcgcctgcg cttgcagcag tgccggacag
    10321 cgagtgggta tgagcaccat ctgatgatgt cttccctgca ttacgagtct ggccactacc
    10381 gcttgttgtg ctcataatcc ccgcgcttag atttgaaatc gcggtataac cattagggaa
    10441 aatgctcgtg ttcgtgccac caaatgcacc ggaactcttg tgttggtgcg caccggcact
    10501 atttgcagtc ccgctaatac tatgggtatg cgccccggtg ttattcgtgg atttggttcc
    10561 gtaatcaaac gacgatgtgt ttttcgtccc caaatccgta ctggatgcgc tggcgctgtg
    10621 ggtgtgcgat ttaatgccgt cctgttcctg agacaatacg gcacgaccac tggcgggttt
    10681 gcccttaatc gtccagccac gcatatcagg gatcacgcct gacggataag cggctgcaag
    10741 tttcgggtat gcagatttgt caaaagtctg cccctgcatc agggcatagc cagacggaac
    10801 ggtatctgat ggccacggga ttggtgcgcc aggcggataa aactgctctg atggcgtata
    10861 gagtgaataa actgtaccgt ccgttaaccc ttccggctta ttagcagaat atgctggtga
    10921 cgtatgaatc gtcacgctgg cattactggt ataatcccat tgaatattta caccagtcgc
    10981 ataatttccg attgcaacgt aaatatcgta agtatcacca gatgtattga cccaggcaaa
    11041 atttgtaaac cctgtcgatg tgcgctgcca taaagcacca gtaatcccct tcggattacc
    11101 attacctgca cgcaaaacaa gttcagatat acctgcctgt tgaggtgacc ccacgttaaa
    11161 tcccgcgcca ccaatcaacg taattgaaac aacagaactc gcctgtggca tggttaccgt
    11221 tgctaatttg aaccaaccag caccaccgct gaatgacatt gttgttgagt taagagtacc
    11281 aatatctttc ggcgtcagtg ttatatcagc tgaaagcgcc ttaccattca ccttacgggc
    11341 agaaggtacc cgaccattcg cattatcatt agctgctttc actgctttcg gtgtcgcggc
    11401 aagcgtttca gatgcgctgt tggttgcact actgagctgg acaattcctt tttgtgttgt
    11461 cgtagcgtcc tgagcagtat atttcccgtt agcaaggtca taggctgcct ttaccgactt
    11521 tggcgttgcc gccagcattt cagatgtgct gttggtcgcg ttgctaagct gaactatccc
    11581 tttctgtgct gtcgttgcat cctgtgcggt gtatttcccg ttagccagct catacgcggc
    11641 cttaacggct tttggcgttg ccgccagtga ctcggaagtg ctgttagtcg cactgctgag
    11701 ctgtactatc ccctttttcg tcgtgctcgc atcctcaagc gccacggcgg atgcaatatc
    11761 ctctgcccgt tttgccgctg tctcggcgcg cgttgccgcg gattccgccg tacttttgct
    11821 ctgtgctgcc gccgtcgcac tgccagctgc ctctgtcgcc ttcgtggatg ctgtcgtggc
    11881 gctgcccttc gctgctgacg cctgtctggt cgcctcatct tttgaagcag acgccgatga
    11941 tgccgatgac gccgccgaac tggctgacga tgcggcagcc gtttttgagg attctgcgct
    12001 ggtttccgac gctttcgcgt tcgtttcgga tgtcttcgct gcggaagcag aactcgctgc
    12061 tgcgctggcc tgttcagtgg cttcgccagc cttcgttgtg gctgttgaag cggatgatgc
    12121 ggcgctttct gccgattttc cggcggcggt ggcactggct gaggcctgcc cggcacttgt
    12181 tgacgcggcg ctggcagatg a
//
LOCUS       DAZGAN020000138        12189 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGAN020000138 DAZGAN020000000
VERSION     DAZGAN020000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.138"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1267
                     /locus_tag="ACHKYO_RS21385"
                     /old_locus_tag="pgaptmp_004277"
     CDS             <1..1267
                     /locus_tag="ACHKYO_RS21385"
                     /old_locus_tag="pgaptmp_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU4431015.1"
                     /translation="KKFSKTILSSVVAGLLLVSSGGVAKEIDIGGKYKVILTEETGSA
                     RIVDINTKKDLFDGIINAKTGTVTTVNKKEIQSAIDSFYAANPKINKDDPNIKQMVAQ
                     AAGNSGRYTVSLPSGFPSLTTENISNLKVTDIEKIKSNIDGVNSVITSKTAKDYNIAI
                     SNGMSSEAALTAAKDGNGALLQEFNRLGTNVNDLKNATTFALDENGEITYEKGVESIP
                     VKDVVAEVKADTTIRKNKDGSYTLDQTAPDTVRVNAAIVDLDKNLKDRTTVGVNSDGS
                     LTTAEGATKTVAVSEGLVSLSGRTDRIDAAVGGIDTRVTKNTQTIQNHSRQLQEHNAR
                     LNSQQRQIRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAVAVGVGY
                     RFNEQTAAKAGIAASDGDVSYNVGVNFEF"
     gene            1355..1828
                     /locus_tag="ACHKYO_RS21390"
                     /old_locus_tag="pgaptmp_004278"
     CDS             1355..1828
                     /locus_tag="ACHKYO_RS21390"
                     /old_locus_tag="pgaptmp_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431016.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRVILAREFFIPENRKSVPTHD
                     ESWVIICDAMVSRLMRCMFQGRRYLQLDAEEITGRLETDRKIRNGDWEQNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGIHTKTVNTFLYMGLGKNGLRYRSVKHLVGRA"
     gene            complement(2119..2451)
                     /locus_tag="ACHKYO_RS21395"
                     /old_locus_tag="pgaptmp_004279"
                     /pseudo
     CDS             complement(2119..2451)
                     /locus_tag="ACHKYO_RS21395"
                     /old_locus_tag="pgaptmp_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332514.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(3291..3821)
                     /gene="yodB"
                     /locus_tag="ACHKYO_RS21400"
                     /old_locus_tag="pgaptmp_004280"
     CDS             complement(3291..3821)
                     /gene="yodB"
                     /locus_tag="ACHKYO_RS21400"
                     /old_locus_tag="pgaptmp_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU4431017.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(4164..4814)
                     /gene="zinT"
                     /locus_tag="ACHKYO_RS21405"
                     /old_locus_tag="pgaptmp_004281"
     CDS             complement(4164..4814)
                     /gene="zinT"
                     /locus_tag="ACHKYO_RS21405"
                     /old_locus_tag="pgaptmp_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HGU4431018.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(5071..5706)
                     /gene="msrQ"
                     /locus_tag="ACHKYO_RS21410"
                     /old_locus_tag="pgaptmp_004282"
     CDS             complement(5071..5706)
                     /gene="msrQ"
                     /locus_tag="ACHKYO_RS21410"
                     /old_locus_tag="pgaptmp_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HGU4431019.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(5707..6711)
                     /gene="msrP"
                     /locus_tag="ACHKYO_RS21415"
                     /old_locus_tag="pgaptmp_004283"
     CDS             complement(5707..6711)
                     /gene="msrP"
                     /locus_tag="ACHKYO_RS21415"
                     /old_locus_tag="pgaptmp_004283"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HGU4431020.1"
                     /translation="MKKNQFLKESDVTAESIFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(6820..7233)
                     /gene="hiuH"
                     /locus_tag="ACHKYO_RS21420"
                     /old_locus_tag="pgaptmp_004284"
     CDS             complement(6820..7233)
                     /gene="hiuH"
                     /locus_tag="ACHKYO_RS21420"
                     /old_locus_tag="pgaptmp_004284"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HGU4431021.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            7366..8037
                     /gene="hprR"
                     /locus_tag="ACHKYO_RS21425"
                     /old_locus_tag="pgaptmp_004285"
     CDS             7366..8037
                     /gene="hprR"
                     /locus_tag="ACHKYO_RS21425"
                     /old_locus_tag="pgaptmp_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HGU4431022.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            8037..9395
                     /gene="hprS"
                     /locus_tag="ACHKYO_RS21430"
                     /old_locus_tag="pgaptmp_004286"
     CDS             8037..9395
                     /gene="hprS"
                     /locus_tag="ACHKYO_RS21430"
                     /old_locus_tag="pgaptmp_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HGU4431023.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINNTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALIKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(9503..10354)
                     /gene="hchA"
                     /locus_tag="ACHKYO_RS21435"
                     /old_locus_tag="pgaptmp_004287"
     CDS             complement(9503..10354)
                     /gene="hchA"
                     /locus_tag="ACHKYO_RS21435"
                     /old_locus_tag="pgaptmp_004287"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HGU4431024.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMKIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYVD"
     gene            complement(10946..12109)
                     /gene="ompC"
                     /locus_tag="ACHKYO_RS21440"
                     /old_locus_tag="pgaptmp_004288"
     CDS             complement(10946..12109)
                     /gene="ompC"
                     /locus_tag="ACHKYO_RS21440"
                     /old_locus_tag="pgaptmp_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU4431025.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLAMMYAETRNMT
                     PYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYVYSKGKDLGGNDYNNNGHQEYVDQDL
                     VNYVEIGATYYFNKNFSTYVDYKINLLDKDDDFYDNNGIATDDVVGVGLVYQF"
BASE COUNT         3445 a         2480 c         2851 g         3413 t
ORIGIN      
        1 caaaaaattc tctaagacta ttctttcttc tgttgttgcc ggactgttgc tggtaagtag
       61 tgggggtgtt gcaaaagaga ttgatattgg tggtaaatat aaagttattc tgacagagga
      121 aacaggttcg gcaaggattg tggatataaa tactaaaaaa gatttatttg atggaattat
      181 taatgcgaaa acaggcactg ttactactgt aaacaaaaaa gagattcaat ctgctataga
      241 tagtttttat gccgctaacc caaaaataaa taaagatgat ccaaacatta aacagatggt
      301 ggctcaggct gctggtaatt cggggcgtta tacagtctct ttacccagtg ggttcccttc
      361 gttaacgact gaaaatattt ccaatctaaa agtgactgat attgaaaaaa taaaatctaa
      421 tattgatggt gttaattcag ttatcaccag taaaactgca aaagattaca atattgctat
      481 cagtaacggc atgagttcag aagctgcgct aacagcggct aaagatggta atggagcgct
      541 gttacaggaa ttcaaccgtc tcggcacaaa tgtaaatgac ctgaaaaatg ccactacatt
      601 tgctcttgat gaaaatggtg aaattaccta tgaaaaagga gtggaaagca ttcctgttaa
      661 ggatgtggtt gcagaggtaa aagccgatac aacaattcgt aagaacaaag atggcagcta
      721 taccctggat cagaccgcgc ctgatactgt tcgtgtgaac gctgcaattg ttgatctgga
      781 taaaaatctg aaagacagaa ccactgttgg cgtgaattca gacggcagcc tgaccactgc
      841 tgaaggagcc acaaaaactg ttgcggtaag tgaaggcctt gtttccctgt cgggaagaac
      901 tgaccgtatt gatgctgcgg ttggtggcat tgacacccgt gtcaccaaaa acactcagac
      961 tatccagaat cactcccgcc agctgcagga gcataacgcg cggctgaaca gtcagcagcg
     1021 tcagattcgt gaaaaccacg aagaaatgaa acgtgctgct gcacagagtg cggcactggc
     1081 aggtttgttc cagccgtaca gtgtgggtaa attcaacgcc acggcagcac tgggtggtta
     1141 cagtgacaaa caggctgtgg ccgttggtgt gggttaccgc tttaacgagc agacagcagc
     1201 caaagcaggt attgcagcca gcgacggtga tgtatcctac aacgttggtg tgaacttcga
     1261 gttttaattg cggacagtga catgaagcat gacggcaggc aggggatttg gcctgccgtt
     1321 tttttctcat gtgagcatga aacagggggc tgcgatgaaa gtgattctgg cgacccgtaa
     1381 ccgttatctg gagtacggtc tgcaacagat gctggaaggt tacagggtga tactggcgag
     1441 ggaatttttt atcccggaga acaggaagag tgtaccgacc catgatgagt cctgggtgat
     1501 tatctgtgat gctatggtga gtcgtctgat gcgctgtatg tttcagggac gccgttatct
     1561 tcaactggat gcggaggaga ttacagggcg tctggaaacg gacaggaaaa tccgtaacgg
     1621 tgactgggaa cagaacacat acgcacgtcc gctgacgatg tcagagatgg tggtgatgtt
     1681 tggttatgta taccgggagt caaagccgtg tcatcttgcc cgtgaaatgg gaattcacac
     1741 gaagacagtg aacacctttc tgtatatggg gcttggtaaa aacggcctga ggtacagaag
     1801 tgtgaagcat cttgtggggc gggcatagcc gcgttttgtt atccgggggt gaagaataat
     1861 ctggtaaatt gtcatgaaac aggcaaatct gaaatacgta aagaagcgct acacagcagg
     1921 agcgatgtgt ttcatgcttt ttgtgtggct gggtacggtg gtaacgctct gtgcagtggc
     1981 tgccttaatg atgcaataaa aaaggggagc tgcacagctc cccgaccaga agaaagaaac
     2041 ttgattacaa taagtgagtt gttaagtcgt cagaataatg gcatacaaaa tatgtcatga
     2101 cagtgatacc gatcgcgttt agtaaagtgg ggttaggccg tattttgctc gcgcacggtt
     2161 acatgcttca ttaaccacac cattgtcgcc ggacatagga aattccctgc aggtggatga
     2221 gcgattttca taaatgctgt agcaggcatt gactcccggt tgaccgctca ggaccacaca
     2281 gcgaggattt ttctggttag tgctctgcat gcatcgatgg aacggagaaa tctgttctgt
     2341 cagtgttacg gggatggtac cgccagcatc atctgcttca gctcagtaaa aagagacgcg
     2401 gaaaaatgca caacaggcac cacacatcac gcatggatta ggattgttca taaattcact
     2461 acgcaagtat tagtaatgat aatggataac agacaatatt ttatcagaaa gaaaaaagta
     2521 agatggtcaa aaatgcccca aagaaagctt ggaatattct tcttaaattt cacgtaattt
     2581 attgattttt cgtgtatgtt tttaagacat ttattccaag aaaaatttat aaccttttga
     2641 ttttacgatt cggtgttatc ggtctcgaaa accggagtag gggcaactct accgggggtt
     2701 caaatccccc tctctccgcc actttatcaa tgacttatct cccgacttcc cgccttgctt
     2761 ttcctaaaca gaacaatcgt agaatattct tgaagggtta gatcgtcact gttttctgtt
     2821 cgatactgtg acattcagca cttgattcgc tatggatctg acaggaagct ttcgagcgaa
     2881 aatgtgcagt tattcagtcg ttttcttatc gctcaccatt attcttttag acattcatcc
     2941 tatgaaactg ccgcaaagtt ggtggtggga actgaagttg cgtagagaag gggtcaataa
     3001 ttgagtcaaa aaaggaacgc ggccgtcaga ttaaagccgc gggacaaagt ccataatttc
     3061 caagagttgt tgaatttccc agcttttttg aaagcctttg tatcatacta acaagtaaaa
     3121 aaggtttaaa taccaaaatt aacctgtatt tttgataagt cttaccattg tacgatttac
     3181 acagtttctg ctcctccttg ttgatagtgg aaagggtgca ttgtgcactt gcgtaaacat
     3241 ttatgacgcg ttgatgtcta tggttcacat ttccttgtca ggggatcgac ttatttgcgg
     3301 cgaggcatca ttcgtaacag agtattatct ttctgaatgt agtgatgaaa gagtgctgcg
     3361 ccagcgtgag ctgcgattaa aaagtaacct atatttgccc aggtttcgtg aatatttttt
     3421 atcagtgctt taatttcgct gtttggggta acaaagggag acacattgaa accaaggaaa
     3481 ctccacgatt ttccactgta agccattaaa gcaatcccca gcagaggaag tgcaaggaag
     3541 gttatataaa gcatgatgtg cattagcgaa gccgctttca tttgccaggc aggtggcggt
     3601 ggtacaatag aaggatcact ataacggtgt tttataatca ggcgtgaaaa cattaacacc
     3661 caaacgaata tacccgcatt gtaatgtgtt tctcgcatca gcagataagt actactacct
     3721 ttaggaaacc agccacggag ttccatcgcg gcataggtta ttgcaacgaa aagcagcgtt
     3781 atccaatgta aataaatttg agtttttgag aatcggttca tttttgtagc tcctgtaatt
     3841 tttacagtca agctatccca cgaaccttaa aaaatcctta tacagatgtg gttttaagaa
     3901 tttgttattt aaaaaattga tagtgcacat tgtaaccagc caatataagc aatggcgtga
     3961 gcacaggtat ctttctctgt tggccgtatt gtgtatggaa tccgttattg gttaactcaa
     4021 tgcgggttag cttattaata tacagacacg tcatagtgat gagcgtgtct gtagctagct
     4081 tgcgttcagt ggcgtttgtg gatggaccag tggcagttat gatgacttac taaagcggca
     4141 tcgaggcgtt atcatgagaa tactcaatga gacatcattt cctcgaccac ttcttcgcta
     4201 ctcaactgat atggataata cgttggccag ttttccattt catttaataa agattgctgt
     4261 gaatcattac ccataaaaat gtgaaaatga gatgattttc gtggtgcaat tatatgatcg
     4321 ctaaattgta tatatttagg ggctttgctt tcaggatctt tacattcgaa taagtaacga
     4381 acgcctttct tgcctgattt ataggtgagt attttgtatc catcgtaatc atatttacag
     4441 gatgttgttt cattatttct atggaattca acaatgccgt cctcaatgcc aatcatctcg
     4501 atatctgttg cataaccttt gtgataataa tctttaattt cagcaaatgt tttagtttta
     4561 tctgcatccg ctttcttctg aaagacgggg tcaagtttgc cactttgcag taaaggataa
     4621 acggattgcc agactccatc ccagtcactg agcgttcggt tttgtacatt ggcatcatca
     4681 aaaacaccat tagcagcttt ttgttcgacc tctgttaagg gtttgccgtg tgagtgatga
     4741 ccatgcgaaa aggcaggagc gctaacaata aagacaccta aagcaacagc cagtttgtga
     4801 agacgaatcg ccaaaacagt ttcctccaga gtcattgtat atgatgtgta atgttatatt
     4861 gtaacatatg acaaaatgca ataactctta ggagatgaga gcatagtttt ctcaggtata
     4921 cttatctatc ttattgaaat aaaatgaatt atgttgataa tttgaggttg atgacaaacg
     4981 aaacataacc tgatgtgatc tgcgtaggcc ggatagggcg ctcgcgccgc atccggcagg
     5041 gataacgcgt actttgtcgg taatatgaag ttatacagat aatttgttgt gaacctgctt
     5101 gcgtaaccgg ttaaaaaggg aacgcaactt cttataccgt aaggctaaaa gcagtacagc
     5161 cagcccagcg tagatgagcg gctgcggtga aataatcttc actgaccaca gataatgtat
     5221 tggggccagg atcgcgacaa gatagacgaa attatgcaac tgttgccagt gcttgccgag
     5281 ttttcgctgc atcgcctggg ttgaagtaaa cgctaaagca agcaaaataa cccagctgat
     5341 aattcctaac gttaaataag gccgggtaat taactccttg cctaataacg ccagattatt
     5401 cacgcccagc tccagcaatg cgtaactggt taaatgcagt gtcgcccagg caaagcacca
     5461 taagcctaac aggcggcgag tgcgtatcaa taacggctgt tttgcgtagc gtgccagagg
     5521 tgtgattaac aaggtcgcca gcaaaaattt cagtgcagtg cgaccggtaa aatgctgaat
     5581 atctttcacc ggatcggcac ccagtccacc gtgattgatc gcccagacca gccagagaaa
     5641 cggcaacaat ccggcaagat gcaggctaac tttcagccat gtcacctgtt ttgctgtcag
     5701 acgcattcag aaattctccc gcaaatccag gccacgatac agcgatgcca cctggtcggc
     5761 gtaaccatta aacagtagcg ttggctggcg ctgaacatcg aggatgccgc ctgaaccaat
     5821 aaatcgttcg gtagcctgtg accagcgcgg gtgatcaaca tgcggattaa cgttggcgta
     5881 aaaaccgtat tcgtcaggcg ctgccagatt ccaggtggtt ggcggacgct cgcgggtcag
     5941 cttaatactg actatcgatt taatcccttt aaagccatat ttccacggca caatcagtcg
     6001 taccggcgcg ccattttgtg gcggtaacgc cttgccataa acacccacgg tcatcagtgt
     6061 gagcggatgc attgcttcgt cgagacgcaa tccttcgaca taaggatatt tcagcccgcc
     6121 gccgataaag cggtcctgct ggccaggcat ctgttccggt gcataaattg tttcgaaagc
     6181 gacatacttc gcattgctgg tgggttcggc aagcgccagc aatttgtgca gcggaaaacc
     6241 aatccacggc accaccatcg accatgcctc cacgcagcgc atacgataaa tacgctcttc
     6301 cagcgggaag cgacgggtta aatcatcgtg atcgagggtc aatggttttg ccacttcgcc
     6361 gctgattttc agtgtccatg gatcggtttt caggctacca gcattagcgg cgggatcggc
     6421 tttatccagc ccgaattcat agaagttgtt ataaccggag actttatcta ctggcgtcag
     6481 tggcaggtta ttttgccagg cggcaggctt gctgaactcc agcggttttc cggcgggggc
     6541 gggcgggcga tcgttccctt taaaccagct aagcagatcg gcatgcgcag cgtgaggcaa
     6601 agaaagtgca gctgcgctga tgcccagtgc ttttaacacc tgtcgacgct tcataaagaa
     6661 tatcgactcg gccgtaacat ctgattcttt taaaaattga ttctttttca taagccatca
     6721 cacttttttg tcgttagcca gcaagagccg tttactgtta atctgtcagg gagtgtcctc
     6781 cctaacagta gcagagatct gcttttacat atttatgcat taactgccac gataggttga
     6841 atacccatat tggctaagta ataaaggcac atgataatgc tcgttcactt tattaatatg
     6901 aaattcaacg gggatctcag ggaagaaact ttcaagattt tgtttcttga aatagtcccc
     6961 ggttttaaat acgacacggt aatcgcccgt agttgcagtt tgctcgggcc acagtgcctt
     7021 aattcgtcca tccttatctg ttttggcggt attaagttgt aaccagccgt tgtccgcttt
     7081 cttttcaaga gtgactgtca cgtcggcagc gggttttccg gtttgctggt tcaaaatgtg
     7141 cacgctaaga atgttttgtt gtgcggcata aaccaaagaa ggtaatgaaa atgctgccgt
     7201 tactacggag agtactaaat aacgctttaa catgtttata tccttgtcat gtgaatgagt
     7261 tttgaggata gcgaaaaagc ggccagggta accttacagc agcattacaa ttttgtaatg
     7321 aaccaggctg tttctataac atatgattta tggcatatta ttttcatgaa gattttactt
     7381 attgaagata atcaaaggac ccaggagtgg gtaacgcagg ggctttccga agcgggttat
     7441 gtcattgatg ccgtttctga tggcagagat gggctttatc ttgcgctgaa ggatgattat
     7501 gcattgatca ttctggatat tatgcttccg ggtatggatg gctggcagat cttacaaacg
     7561 ttaagaacag caaagcaaac ccctgttatt tgccttactg caagggattc tgtcgatgac
     7621 agagtcagag ggctggacag tggagcaaat gattatctgg taaaaccttt ttcattttct
     7681 gagttgctgg caagggttcg ggcacaatta aggcaacatc acgctttgaa ttcaacatta
     7741 gaaatcagtg gcttaagaat ggactctgta agtcaaagtg tgagcaggga caatatcagt
     7801 attacactga cgcgcaagga gtttcagtta ctttggctac tggcctccag agctggcgaa
     7861 attataccca gaacggttat tgcgagtgaa atttggggaa tcaactttga tagtgatacc
     7921 aatacggtgg atgtcgccat tcgcaggctc cgcgcaaaag ttgatgatcc ttttcctgaa
     7981 aagctaatcg ccacaatccg ggggatgggc tattcattcg tagcggtaaa aaaataatga
     8041 aaagattatc tataaccgtc cgtttaacct tgctttttat attgctgctg tctgttgctg
     8101 gcgctggaat tgtctggact ctctataatg gcctggcaag tgagttgaaa tggcgcgatg
     8161 atacaacact cattaaccgg acagcacaga tcaagcagtt gttaattgat ggggtaaatc
     8221 cagatacgtt acctgtgtac tttaaccgga tgatggatgt tagtcaggat atcttgatca
     8281 ttcatggtga tggcatcaat aaaattgtta accggacaaa tgtcagtgat ggcatgttaa
     8341 ataacatacc tgctagtgag acaatcagcg cagctggcat ttacagaagc attattaata
     8401 acacagagat agatgcttta cgaattaata ttgatgaagt ttcgccatca ttaacggtta
     8461 ctgtggctaa attggcttca gccagacata acatgcttga acagtataaa attaatagca
     8521 ttataatttg cattgtcgcc attgtacttt gctcagtatt aagtccgctg ttaatcagaa
     8581 cggggttacg agagatcaaa aagttgagtg gtgtaacgga agcgctgaat tataacgata
     8641 gccgggagcc tgttgaggtt agcgcattac cgagagaact aaaacctctt gggcaggcgt
     8701 tgaataaaat gcatcaagcc ttaatcaaag attttgagcg actaagtcag tttgctgacg
     8761 atctcgctca tgaacttaga acgccaatta atgcattact gggtcagaat caggttacgc
     8821 tcagtcaaac cagaagtatc gctgaatatc aaaaaacaat tgccggtaac attgaagagc
     8881 tggaaaatat ttcgcggtta acagagaaca tactgtttct tgccagggca gataaaaaca
     8941 atgttttggt gaaactggac tcgctttctc tcaataagga agtcgaaaat ttgttggact
     9001 atcttgaata cctttcagac gagaaagaga tttgctttaa ggtcgagtgc aatcagcaaa
     9061 tctttgcgga taaaatttta ctgcaacgaa tgttatcgaa tcttattgtt aacgccattc
     9121 gatattcgcc agaaaaatcg cgcattcata taaccagttt tcttgatacc aacggctatc
     9181 ttaatattga tgtcgccagt cctggaacga aaattcatga gcctgaaaaa ctcttccgta
     9241 gattttggcg gggagataat tcgcgtcatt ccgtaggtca gggacttggt ctttctttag
     9301 tcaaagcgat tgccgaattg catgggggaa gtgctacgta tcactatctc aataagcata
     9361 atgtgttccg gattatgtta ccgcaaagaa attaacatag ggcttcagta cgccataaat
     9421 caaccatgaa aaaacgcagc cacgtcgtga gtattaacgt ggctacgatt gattatgcgt
     9481 ttacattcaa acgtaacagg gattaatcca cataagctgc cagcatttcc tgcgccgcca
     9541 gtttacccaa cgcattcgct gcgaaaggac tgtcgccggt gagaagttta cggtccttat
     9601 gtactcgccc ggtgatgtcg tcattaatga ttttcatgcc cattttcttc agctcttcgc
     9661 cgaagtacca ggtgagatga cctggcatat agccaatatc tggcgtttgt ttgtctgcgg
     9721 cgtctgggaa tgcgcaaatg gaataaccat tcagtgggtt atcgccgtgg cgaagcgcca
     9781 gaaaagccgc cgggccgtgg caaagggaga taacaaaacg gtcatttttg attgcccact
     9841 gtaaagcggc agctacgtcc tggctttcag gtaaaccaat aagtgcgcca tgaccaccag
     9901 gaacaaagat tgctgcatat tcgctatcag cgttgaggct ggcgacaaca tccgcgagtt
     9961 tcttcggatt gcggaacaac gatttatgct gctcaaagaa tggcattact ttttcatctt
    10021 tgtgcggcat agcccagtat tcaaacttgg tcatcagacc ggaaatggtc gccacttcga
    10081 attcgaaacc tgcagcatgg agatgataca acggcagcaa cgtttcaatc ggatggttac
    10141 cggtcgagaa cagttttccg ttatcggttg gcaaataacg ttcgtctgcg gcgatcacca
    10201 gaattttatg tttaccgcga tacggttttg gatagtccac gccatcaaga tcagagacag
    10261 gactggtata ttggctaagc gaatattctg aagggaagaa tgcattatct tcagcaatat
    10321 cgacctgcgg atttttactt gtttgaacag tcatagtgta ttccttattg ttgcttagtt
    10381 agggtagtca ctatatttgc gactgagctg gtcgctattt ttaaccaggc tggaaaattg
    10441 agtcaatata tcgaaagcgg tagtcggcag gtgtaagtat gtaatgatga tgctgcactg
    10501 gtgttgaata ttattcacgc caaattttcc ttattgagca acgtcgtcgc aagtggcctc
    10561 aattatttta gaccattatc agaggtacgg ggcgacgggg agagatttag tgctgtggaa
    10621 tattgtggtg ctgatgaata tttatatccc ggattggtag agaaaaagct tatttcatgc
    10681 actatttttg caaatatttc tatatgggag gcggttttgt tgcagattaa ttcataaaga
    10741 aatgcatttg ggtcttggct ggaagagtcg ctggtagatg ttattgtgcc agcaggggca
    10801 gggtaaggtg ctgtatcgat atgcattggc aattgcgtgt gccgcagatg gagatgaaat
    10861 gccaataaaa aagcctgaat tcgatgaggg acgaactcag gctagtgttt agttttacag
    10921 taacacgccg cagcacgttg gctgattaga actggtagac taagcccaca ccaacaacgt
    10981 catctgttgc gatgccattg ttgtcataga aatcgtcatc tttatccaac aagtttattt
    11041 tatagtcaac atatgttgag aagttcttat tgaaatagta ggtcgctcca atttcaacat
    11101 agttgaccag atcctgatca acatactctt gatgaccatt gttgttatag tcgttaccgc
    11161 cgaggtcttt gccttttgaa taaacatatg caagagatgg gcgcagacca aaatcgaact
    11221 gatactgtgc tacagcttca aagttctggg ttttatttgc gatacccaca ttaccatatg
    11281 gtgtcatatt gcgggtttca gcatacatca ttgcaagata aacattgttg gcgtcgtatt
    11341 ttgcaccaaa tgtccatgct tcagctgtat caccaccagc aaatttgctg tattcattgt
    11401 agtgataccc gccaagagct acttgagaat tggtacggtc agagtttgag taagcggcac
    11461 ctaagctcaa accgaagtca aagtcatatg aagtggaaag accaaaacca tcaccgttct
    11521 ggaagcgaac gtcttcgtga ccatttttag taccttcttg atcttctcct gcattgctgc
    11581 cttcgttgtt accttgatac tgcaatgcaa agttcagacc ttccaccaga ccgaagaaat
    11641 cactattgcg gtaagtagct accccatttg cacggcctgc catgaagtta tcagtccagg
    11701 tataggagtc gccgccgaat acaggaagca tatctgtcca ggcttcaacg tcgtaaatca
    11761 caccatagtt acgaccatag tcaaaggagc ccatctcgcc gaacttcaga cctgcaaaag
    11821 caagtttgac ataagtatta tcagaacctt cagtgtcgct agcctttgtt ttggtcagtg
    11881 cttgaccata accagttaac tgatctgtaa tttgggtctc acctttgaag cctaactcga
    11941 tgatagtttc atcgccgtca tcgccagggt tatcggaaaa agtatgacga gcatcaactt
    12001 ttccgtacag atccagttta ttgccatttt tattataaat ttcagccgca tttgctgcgc
    12061 cagcaactaa taacgccggg accagcattg ccagaacttt tcttttcatt atgtattccc
    12121 ttgtgattat aatcttcatg aatatatcaa taagtgccgt tatccaaaaa aaagcacatt
    12181 tggatacta
//
LOCUS       DAZGAN020000139        12114 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGAN020000139 DAZGAN020000000
VERSION     DAZGAN020000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.139"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2091
                     /locus_tag="ACHKYO_RS21445"
                     /old_locus_tag="pgaptmp_004289"
     CDS             <1..2091
                     /locus_tag="ACHKYO_RS21445"
                     /old_locus_tag="pgaptmp_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HGU4431026.1"
                     /translation="LKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQRS
                     ITAPDRIGEGKSWVYQRDTMIERGDNSGVHQYGPAEHFTHIISDKPSPKDEYIAYAIN
                     IPDYELAADVYNINVTSPSEQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKK
                     PGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAY
                     QPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADI
                     RAKISKDIEGGIPSYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKD
                     EWENLHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQI
                     ERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYERDDLS
                     AIDFSLLMKTIKAFSFGGDLQTLASKPGSTISSIPSERRILININHDFPNNGNLFNDF
                     LFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCS
                     TYQQLYNEFHALYIDKAGIWSAFEGIFATKNVLAFSREDSVNINHSPHDGLVIINKGN
                     EEVEGTWPNKLQPGIYKNMGSNSVNIIINNTQKIIPPGKVFTLRGGSLNINIPGRSAL
                     LLGKTGEPPNYLYL"
     gene            2427..3404
                     /gene="glaH"
                     /locus_tag="ACHKYO_RS21450"
                     /old_locus_tag="pgaptmp_004290"
     CDS             2427..3404
                     /gene="glaH"
                     /locus_tag="ACHKYO_RS21450"
                     /old_locus_tag="pgaptmp_004290"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HGU4431027.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3423..4691
                     /gene="lhgO"
                     /locus_tag="ACHKYO_RS21455"
                     /old_locus_tag="pgaptmp_004291"
     CDS             3423..4691
                     /gene="lhgO"
                     /locus_tag="ACHKYO_RS21455"
                     /old_locus_tag="pgaptmp_004291"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HGU4431028.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYRPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4714..6162
                     /gene="gabD"
                     /locus_tag="ACHKYO_RS21460"
                     /old_locus_tag="pgaptmp_004292"
     CDS             4714..6162
                     /gene="gabD"
                     /locus_tag="ACHKYO_RS21460"
                     /old_locus_tag="pgaptmp_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4431029.1"
                     /translation="MKLNDSKLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLLMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALSSKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6176..7456
                     /gene="gabT"
                     /locus_tag="ACHKYO_RS21465"
                     /old_locus_tag="pgaptmp_004293"
     CDS             6176..7456
                     /gene="gabT"
                     /locus_tag="ACHKYO_RS21465"
                     /old_locus_tag="pgaptmp_004293"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HGU4431030.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            7694..9094
                     /gene="gabP"
                     /locus_tag="ACHKYO_RS21470"
                     /old_locus_tag="pgaptmp_004294"
     CDS             7694..9094
                     /gene="gabP"
                     /locus_tag="ACHKYO_RS21470"
                     /old_locus_tag="pgaptmp_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HGU4431031.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9115..9777
                     /gene="csiR"
                     /locus_tag="ACHKYO_RS21475"
                     /old_locus_tag="pgaptmp_004295"
     CDS             9115..9777
                     /gene="csiR"
                     /locus_tag="ACHKYO_RS21475"
                     /old_locus_tag="pgaptmp_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HGU4431032.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9778..10227)
                     /gene="kbp"
                     /locus_tag="ACHKYO_RS21480"
                     /old_locus_tag="pgaptmp_004296"
     CDS             complement(9778..10227)
                     /gene="kbp"
                     /locus_tag="ACHKYO_RS21480"
                     /old_locus_tag="pgaptmp_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HGU4431033.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10311..10469)
                     /locus_tag="ACHKYO_RS21485"
                     /old_locus_tag="pgaptmp_004297"
     CDS             complement(10311..10469)
                     /locus_tag="ACHKYO_RS21485"
                     /old_locus_tag="pgaptmp_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HGU4431034.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10652..10951
                     /locus_tag="ACHKYO_RS21490"
                     /old_locus_tag="pgaptmp_004298"
     CDS             10652..10951
                     /locus_tag="ACHKYO_RS21490"
                     /old_locus_tag="pgaptmp_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGU4431035.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10961..11485
                     /gene="ygaP"
                     /locus_tag="ACHKYO_RS21495"
                     /old_locus_tag="pgaptmp_004299"
     CDS             10961..11485
                     /gene="ygaP"
                     /locus_tag="ACHKYO_RS21495"
                     /old_locus_tag="pgaptmp_004299"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HGU4431036.1"
                     /translation="MALTTISPHNAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(11532..11936)
                     /gene="stpA"
                     /locus_tag="ACHKYO_RS21500"
                     /old_locus_tag="pgaptmp_004300"
     CDS             complement(11532..11936)
                     /gene="stpA"
                     /locus_tag="ACHKYO_RS21500"
                     /old_locus_tag="pgaptmp_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HGU4431037.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         3038 a         2972 c         3109 g         2995 t
ORIGIN      
        1 ttaaaagaca gagaaattat atcaactttt cacgcagaaa aagttattaa tgatctgtta
       61 aaaaacgatt ataaaatttc cctttctcca gatacaactt atcgagagtt gaataaagca
      121 gcacagcgta gcattacagc gccagacagg ataggagaag gaaaatcatg ggtttatcaa
      181 cgagatacaa tgattgaaag aggtgataac agcggtgttc atcagtatgg tcctgctgaa
      241 catttcaccc acattatatc tgacaaaccg tccccaaaag atgagtatat tgcatatgct
      301 attaacattc ctgactatga gctggcagcc gatgtatata atattaacgt gacgtcacct
      361 tccgaacagc aagaaacatt taaaatacta atcaatccag aacatctacg gcaaacactt
      421 gagcgtaaat ctcttactgc tgttcagaaa tcacaatgtg aaatcataac ccccaaaaaa
      481 cctggcgaag cgattcttca tgcttttaat gccacctacc agcaaatcag ggaaaatatg
      541 tctgagtttg cacgttgcca ttatgggtat atacaaatcc ctccagtgac aactttccgc
      601 gccgacggac cagaaactcc cgaagaagaa aagggttact ggtttcatgc ttatcaaccc
      661 gaagatcttt gtaccatcca taatccaatg ggagatttgc aggattttat tgcattggtt
      721 aaagatgcta aaaaatttgg tatcgatatc attcctgatt atacctttaa ctttatggga
      781 attgggggta gtggtaaaaa tgacctggat tatccctctg ctgatatacg agcgaagatc
      841 agtaaagata tagaaggtgg tatccctagc tattggcaag gtcaggtttt gattccattc
      901 attaaagatc cagtaacaaa agaacgtaaa caaatccatc cagaagatat acatctcact
      961 gcaaaagact tcgaagcaag taaagataac atctctaagg atgaatggga aaacctccat
     1021 gcattaaaag aaaagcgttt aaatggaatg cctaaaacaa cacccaaaag tgaccaggtt
     1081 attatgttgc aaaatcaata cgttcgtgaa atgcgaaaat atggcgtacg aggtttacgt
     1141 tatgatgcgg caaaacactc aaaacatgaa caaatagaaa gatcaataac cccaccgctt
     1201 aaaaattata atgagcggtt acacaatact aacttattta acccaaaata tcataaaaaa
     1261 gccgttatga attacatgga atatctggta acttgtcagt tggatgaaca acaaatgtca
     1321 tcactgcttt atgaaagaga tgatttaagc gccattgatt tttcattgct catgaaaacg
     1381 ataaaagcct tttcatttgg tggagatctt caaacccttg catcaaaacc gggttccaca
     1441 atctcaagta tcccatcaga aagacggata ttgattaaca ttaaccacga ttttcctaac
     1501 aatggtaatc ttttcaatga ctttctattt aaccatcaac aagatgaaca attagcaatg
     1561 gcatatatgg ccgctctgcc gttcagcagg cctttagttt actgggatgg ccaagtatta
     1621 aaatcaacga ctgaaattaa aaattatgat gggtccacgc gtgttggcgg tgaggcgtgg
     1681 cttaataaag gttgctctac ctatcagcag ctctacaatg aattccacgc attatatata
     1741 gataaagcag gaatatggag cgcatttgag ggtatatttg caactaaaaa cgttctggcc
     1801 tttagtcgtg aggattctgt gaacattaat cactctcctc atgatggact agttataata
     1861 aataaaggaa acgaagaagt tgaaggtacc tggcctaaca aattgcaacc tggaatatat
     1921 aaaaacatgg ggagtaatag cgttaacatt attattaata atacccaaaa aattatcccc
     1981 cctggtaaag tatttacgct tagaggcgga tctctaaata tcaatattcc tgggcgtagc
     2041 gctcttcttt tagggaaaac aggagaaccg ccgaactatc tctatttata atttaatgtt
     2101 atatctgccc cgataaaacg gggcagataa tatgtttagt ttactaacgg tcattttgca
     2161 gtgaagccat ttactgtttt ttatcgacca gataatctgt tctctaatgt taactccccc
     2221 taacctgttg ctttagttat tcatttcctg tctcactttg ccttaatacc ctacgttaaa
     2281 tgttactaat ttgttgcttt tgatcacaat aagaaaacaa tatgtcgctt ttgtgcgcat
     2341 ttttcagaaa tgtagatatt tttagattat ggctacgaaa tgagcatcgc catgtcaccc
     2401 tacatctcat aagaggatcg cttctgatga atgcactgac cgccgtacac aataacgctg
     2461 tcgattcagg ccaggactat agcggattca ctctcatccc gtcggcgcaa tccccgcgtc
     2521 tgctggaact caccttcacc gaacagacga ccaaacagtt tctcgaacag gttgccgaat
     2581 ggcccgtgca ggcgctggag tacaaatcgt ttctgcgttt tcgggtaggc aaaattcttg
     2641 acgatctgtg tgcgaatcag ctgcaaccat tgctgttgaa gaccctgcta aaccgtgctg
     2701 aaggtgcgct gttgatcaat gcggtgggta tcgatgatgt ggcgcaggca gatgagatgg
     2761 tgaagctggc aacggcggta gcgcatctga ttggtcgctc aaatttcgat gcgatgagcg
     2821 gtcagtatta cgcgcgtttc gtggtgaaaa atgtcgataa ctcagacagc tatctgcgtc
     2881 agccgcaccg cgtgatggaa ctgcacaacg acggcacgta tgtcgaagag atcaccgatt
     2941 acgtgctgat gatgaaaatc gatgagcaaa acatgcaggg tggaaattcg ttgctgctgc
     3001 atctcgatga ctgggaacat ctggaccact atttccgcca tccactggcg cgtcgcccaa
     3061 tgcgctttgc cgcgccgccg agcaaaaacg tcagcaaaga tgttttccat ccagtgttcg
     3121 acgttgatca acagggtcgc ccggtgatgc gctatatcga ccagttcgtc cagccgaaag
     3181 acttcgaaga aggcgtgtgg ttgagcgagc tttcggacgc cattgaaacc agcaaaggca
     3241 ttctttctgt acccgttccc gttggcaaat tcctgttgat aaacaacctg ttctggctgc
     3301 acggtcgcga ccgctttact ccgcacccgg atctgcgccg tgaactgatg cgtcagcgcg
     3361 gctacttcgc ttacgccact caccactacc agacgcatca gtagcgcaaa ggaattgagc
     3421 ggatgtatga ttttgtgatt attggcggtg gcatcatcgg catgtcgacc gccatgcaac
     3481 tgattgatgt ctacccggac gcccgcattg cgttgctgga aaaagagtcc ggcccggcct
     3541 gtcaccagac gggccacaac agcggcgtga tccatgccgg ggtttattac aggcccggta
     3601 gcctgaaggc acagttttgc ctggcgggaa accgcgccac taaagccttt tgcgatcaaa
     3661 acggcattcg ctatgacaac tgcggcaaga tgctggtcgc cacctctgaa ctcgaaatgg
     3721 aacggatgcg cgcgttgtgg gaacgcacgg cggcaaacgg tatcgagcgc gagtggttaa
     3781 acgccgtgga actgcgcgag cgcgaaccga atatcaccgg gcttggcggc atttttgtgc
     3841 cgtccagcgg cattgtcagc taccgcgaag tcacagcggc gatggcaaaa attttccagg
     3901 ccagaggcgg tgagattatt tataacgccg aagtcagcgc cctcagtgag cataaaaacg
     3961 gcgtggtcgt ccgaacccgt cagggcggcg aatatgaagc atcgacgctg attagctgtt
     4021 ccgggctgat ggctgaccgg ctggtgaaaa tgctcggcct cgaaccgggt tttattatct
     4081 gcccgttccg tggcgagtat ttccgccttg cgccggagca taaccagatt gttaaccatc
     4141 tgatttaccc cattcccgac ccggcaatgc cgtttttggg cgttcatctc acccgcatga
     4201 tcgacggcag cgtgaccgtc gggccaaacg cggtgctggc tttcaaacgc gaaggctatc
     4261 gcaagcgcga cttctcattt agcgacacgc tggagatttt gggctcgtcg gggattcgcc
     4321 gggtgctgca aaaccatcta cgctcaggac tgggcgagat gaaaaactcg ctgtgcaaaa
     4381 gcggctatct gcggctggtg caaaagtatt gtccccggct ttcgttaagc gatctccagc
     4441 cctggcccgc cggtgtgcgg gcgcaggcgg tatcgccgga cggcaagctg attgacgatt
     4501 ttctgtttgt caccaccccg cgcacgatcc acacctgcaa tgcgccctcc ccggcagcga
     4561 catcagcaat tcctattggt gcgcatattg tcagcaaggt acaaacgctg ttggcaagcc
     4621 agagtaaccc cggacgcacg ctgcgagcgg cacgtagtgt ggatgcctta cacgccgcat
     4681 ttaatcaata acctttgaaa acaggacgta acaatgaaac ttaacgacag taaattattc
     4741 cgccagcagg cgttgattaa cggggaatgg ctggacgcca acaatggcga ggtcatcgat
     4801 gtcaccaatc cggcgaacgg cgacaagctg ggtagcgtac ccaaaatggg cgctgatgaa
     4861 acccgcgccg ctatcgacgc cgccaaccgc gctctgcccg cctggcgtgc gctcaccgcc
     4921 aaagaacgcg ccaacattct gcgcaactgg ttcaatttgt tgatggagca tcaggacgat
     4981 ttagcgcgcc tgatgaccct agaacagggc aaaccgctgg ctgaagcgaa aggcgaaatt
     5041 agctacgccg cctcctttat tgagtggttt gctgaagaag gcaaacgcat ttatggcgac
     5101 accattcctg gtcatcaggc cgataagcgc ctgattgtta tcaagcagcc gattggcgtc
     5161 accgcagcta tcacgccgtg gaacttcccg gcggcgatga ttacccgcaa agccggtccg
     5221 gcgctggcgg caggctgcac catggtgctg aagcctgcca gtcagacgcc gttctctgcg
     5281 ctggcgctgg cggagctggc gattcgcgcg ggcattccgg ctggggtatt taacgtggtc
     5341 accggttcgg cgggcgcggt gggtaacgaa ctgaccagca acccgctggt gcgcaagctg
     5401 tcgtttaccg gttcgaccga aattggccgc cagttaatgg aacagtgcgc gaaagacatc
     5461 aaaaaagtgt cgctggagct gggcggcaac gcgccgttta tcgtctttga cgatgccgac
     5521 ctcgacaaag ccgtggaagg tgcgctgtcc tcgaaattcc gcaacgccgg gcaaacctgc
     5581 gtctgcgcca accgtttata cgtgcaggac ggcgtgtatg accgctttgc cgaaaaattg
     5641 cagcaggcgg tgagcaaatt gcacatcggc gacgggctgg agaaaggcgt caccatcggg
     5701 ccgctgatcg atgaaaaagc ggtagcaaaa gtggaagagc atattgccga tgcgctggag
     5761 aaaggtgcgc gtgtggtttg cggcggtaaa gcggatgaac gcggcggcaa cttcttccag
     5821 ccgaccattc tggtggacgt tccagccaac gccaaagtgt cgaaagaaga gacgttcggc
     5881 cccctcgccc cactgttccg ttttaaagat gaagccgatg tgattgcgca agccaatgac
     5941 accgagtttg gccttgccgc ctatttctac gcccgtgatt taagccgcgt cttccgcgtg
     6001 ggcgaagcgc tggagtacgg catcgtcggc atcaataccg gcatcatttc caatgaagtg
     6061 gccccgttcg gcggcattaa agcctcgggt ctgggtcgtg aaggttcgaa gtatggcatc
     6121 gaagattact tagaaatcaa atatatgtgc atcggtcttt aactggagaa cgcgaatgag
     6181 cagcaataaa gagttaatgc agcgccgcag tcaggcgatt ccccgtggtg ttgggcaaat
     6241 tcacccgatt ttcgctgacc gcgcggaaaa ctgccgggtg tgggacgttg aaggccgtga
     6301 gtatcttgat ttcgcgggcg ggattgcggt gctcaatacc gggcacttgc atccgaaagt
     6361 ggtggctgcg gtggaagcgc agttgaaaaa actgtcgcac acctgcttcc aggtgctggc
     6421 ctacgagccg tatctggagc tgtgcgagat tatgaatcag aaggtgccag gcaattttgc
     6481 caagaaaacg ctgctggtca ccacaggttc tgaagcggtg gaaaacgcgg tgaaaatcgc
     6541 ccgcgccgcc accaaacgta gcggcaccat cgcttttagc ggcgcgtatc acgggcgcac
     6601 gcattacacg ctggcgctga ccggcaaggt gaatccgtac tctgcgggca tggggctgat
     6661 gccgggtcat gtttatcgcg cgctttatcc ttgcccgctg cacggcataa gcgaggatga
     6721 cgctatcgcc agcatccacc ggatcttcaa aaatgatgcc gcgccggaag atatcgccgc
     6781 catcgtgatt gagccggttc agggcgaagg cggtttctac gcctcgtcgc cagcctttat
     6841 gcagcgttta cgcgctctgt gtgacgagca cgggatcatg ctgattgccg atgaagtgca
     6901 gagcggcgcg gggcgtaccg gcacgctgtt tgcgatggag cagatgggcg ttgcgccgga
     6961 tcttaccacc tttgcgaaat cgatcgcggg cggcttcccg ctggcgggcg tcaccgggcg
     7021 cgcggaagta atggatgccg tcgctccagg cggtctgggc ggcacctatg cgggtaaccc
     7081 gattgcctgc gtggctgcgc tggaagtgtt gaaggtgttt gagcaggaaa atctgctgca
     7141 aaaagccaac gatctggggc agaagttgaa agacggattg ctggcaatag ccgaaaaaca
     7201 cccggagatc ggcgacgtac gcgggctggg ggcgatgatc gccattgagc tgtttgaaga
     7261 cggcgatcac aacaagccgg acgccaaact caccgccgag atcgtggctc gcgcccgcga
     7321 taaaggcctg attcttctct cctgcggccc gtattacaac gtgctgcgca tccttgtacc
     7381 gctcaccatt gaagacgctc agatccgtca gggtctggag atcatcagcc agtgttttga
     7441 tgaggcgaag cagtagcgcc gctcctatgc cggaggcgac gctgcgcgtc ttgtccggcc
     7501 tacggggatc caggtcggat aaggcgttta cgccgcatcc gacaatctgt acgtgaacag
     7561 gaagaaatct atgttggccg ggtaaggcgg agccgctctc cggcaaaaag aatcaataac
     7621 aattatacgc gtgacccggc gcgggaaatg tcggggcgct ctcccaagtg acacactttc
     7681 gagaggattc aggatggggc aatcatcgca accacatgag ttaggcggcg ggctgaagtc
     7741 acgccacgtc accatgttgt ctattgccgg tgttatcggc gcaagtctgt ttgtcggttc
     7801 cagcgtcgcc atcgccgaag cgggcccggc ggtattactg gcctatctgt tcgccgggct
     7861 actggtggtt atgattatgc ggatgttggc ggaaatggcg gttgccacgc ccgataccgg
     7921 ttcgttttcc acctatgccg ataaagccat tggtcgctgg gcgggctata ccatcggctg
     7981 gttgtactgg tggttttggg tactggttat cccgctggaa gccaacatcg ccgccatgat
     8041 cctgcactca tgggttccag gcattcccat ctggttattt tccctcgtca ttaccctcgc
     8101 cttaactggc agtaacttat taagcgttaa aaactacggc gaatttgagt tctggctggc
     8161 gctgtgcaaa gtcatcgcta tcctggcctt tattttcctt ggtgcagtcg caattagcgg
     8221 tttttacccg tatgccgaag tgagcgggat ctcaagattg tgggatagcg gcggctttat
     8281 gcccaacggt ttcggtgcgg tattaagcgc gatgttgatc accatgttct cgtttatggg
     8341 cgcagaaatt gtcaccattg ccgccgcgga atccgacacg ccggaaaaac atattgtccg
     8401 cgccaccaac tcggttatct ggcgtatttc tatcttctat ttatgttcta tttttgtcgt
     8461 cgtggcatta attccgtgga atatgcccgg actaaaagcc gtcggttctt atcgctcggt
     8521 actggaattg ctcaatattc cccatgcgaa attaatcatg gactgcgtga tattactttc
     8581 cgtaaccagt tgcctgaact cggcgctgta taccgcgtca aggatgctct actccttaag
     8641 ccgtcgcggt gatgctcccg cggtaatggg taaaatcaac cgcagtaaaa ccccgtacgt
     8701 ggcggtgtta ctctccaccg gcgcggcatt cttaacggtg gtggtgaact attacgcgcc
     8761 tgcgaaggta tttaaatttc tgatcgacag ctccggcgct atcgccctgc tggtttattt
     8821 agtcatcgcc gtttcacagt tgcggatgcg caaaattctg cgagcagaag gaagcgaaat
     8881 tcgcttgcga atgtggcttt atccgtggct cacctggcta gtcatcggct ttattacctt
     8941 tgtgttggta gtgatgctat tccgtccggc gcaacagtta gaagtgatct ccaccggctt
     9001 attagcgata gggattatct gtaccgtgcc aattatggct cgctggaaaa agctggtatt
     9061 gtggcaaaaa acacccgttc ataatacgcg ctgatcatga tcaggagtca caccatgacc
     9121 attacgtctc tggatggcta tcgctggctg aagaacgata ttattcgcgg taattttcaa
     9181 ccggatgaaa aattacgaat gagtttgctg acatcgcgtt atgcacttgg cgttgggccg
     9241 ttacgggaag ctctttcgca actggtggcg gaacggctgg tcacggtggt gaatcaaaaa
     9301 gggtatcggg tggcgtctat gtcagagcag gagctgctcg atattttcga cgcccgcgcc
     9361 aatatggaag cgatgttagt gagtctggcg attgcccgcg gtggcgatga gtgggaggca
     9421 gacgttctcg caaaagcgca tctgctgagt aagcttgagg cctgtgacgc cagcgagaaa
     9481 atgcttgatg agtgggatct gcgtcatcag gcgtttcata cggcaattgt ggcgggctgt
     9541 ggttctcact atttgctgca aatgcgtgaa cggttgtttg atctggcggc gcgctatcga
     9601 tttatctggc tgcggcgaac ggtgctttcg gtagaaatgc tggaggataa acacgatcag
     9661 caccagaccc tgactgcggc ggtactggcg cgagataccg cgcgcgccag tgagttaatg
     9721 cgccagcatt tactgacgcc aattcccatt atccagcagg cgatggctgg caattaacta
     9781 ctcttccgga atacgcaaca cttgccccgg ataaatttta tccgggcttt ttagcatcgg
     9841 tttattcgct tcgaagattt tattgtacag attagcgtta ccgtagacct gtttggaaat
     9901 ggcactcaga gtgtcgccag acttaacggt ataaaactgg ctggcagtgg ctggtgtcgc
     9961 cgttttcacc tgatcatcaa cactggcaat accggaaata ttcccaaccg caacaaggat
    10021 tttctccttc gcctcctgac tcaggccgtc accagtgacc gtcgctttgc cgtcggcaat
    10081 ttgaatgttc actttatcgg catcaggtat accggttttg ctcagatgct cctgcacctt
    10141 cttcgcctga tcgtctttat cgtgctgacc tgtaaccgcg tcccagagtt tttctccggc
    10201 atctttcaca aaattgaaca gacccatagc tacctcattc gttaacggaa acactcagaa
    10261 agtgtagcag aggaacgcgg cgcgttaacg ccgcgggggt gagcacaggt ttaatcgcgg
    10321 gtttgcaccc agaacgcgtg aatcaaaccg gggatatacc caagcagtgt cagcagaata
    10381 ttaataatga acgcccaacc gaacccttta ccgagcagca cgccgagcgg cggcagaatg
    10441 atggtgatga cgattctcca gaaacccata tgtactccct ataagaaaat tactcattgt
    10501 ttaaaaagag attttatctc ttaagcgtag taattttagc ggagcctgcc agtttcagct
    10561 tacgctttta ctctcattta cgctgctgag gctggtatat gttgcatttt tagtttagag
    10621 tttacttaat ttagaaaata cttaaataaa tatgactgaa ctcgcgcaat tacaggccag
    10681 tgccgaacag gcagcggcct tattgaaagc aatgagccat cctaaacgat tgctgattct
    10741 gtgcatgctt agcggttccc ccggcaccag cgcgggagag ctgacgcgca ttaccggact
    10801 gagtgcctct gcgacatcac agcatctcgc tcgtatgcgg gacgaagggc ttatcgacag
    10861 ccaacgggat gcccaacgca ttctatattc cattaaaaat gaggcggtaa atgccattat
    10921 cgccaccctg aaaaatgtct attgtccgta aggagtcact atggctttga caaccatttc
    10981 gccgcataat gcacaagaat taatcgcacg cggcgcaaag ttaatcgata ttcgtgacgc
    11041 tgatgaatat cttcgtgaac atattcctga agcagatctg gctccattat ccgtgctgga
    11101 acagtcaggt cttccaccta aattacgtcg cgagcaaatt atattccact gccaggcagg
    11161 taaacgcacc agtaataacg ccgataaatt agcagcgatt gccgcccccg cagaaatatt
    11221 tttactcgaa gatgggattg atggctggaa aagagctgga ttgccagtag cggtaaataa
    11281 atctcaaccc ttgccgttaa tgcgccaggt gcagatcgct gcgggtggtt taatattaat
    11341 cggcgttgta ctgggttata ccgtaaatag cggtttcttc ttattaagtg gctttgttgg
    11401 tgccgggtta ctgtttgcag gaatcagcgg tttttgtgga atggcaaggt tgttagataa
    11461 gatgccgtgg aaccaacgaa cttgagaagc gacgccggac gcgccctagc agcgacatcc
    11521 ggcctcagta attagatcag gaaatcgtcg agagatttac cttctgccag cgcctgagca
    11581 attggcttcg gtgtacggcc ctgaccggtc caggttttag tttcaccgtt aacatcggtg
    11641 aatttatatt tcgccggacg cggctggcgt tttttaccag cgcgtggtgc agcagcagag
    11701 ctattaccta ataactcttc cgggttaatt ccgtcagcct tcatcagctc cagccaggtg
    11761 ctaatttttt cctgacgctc agccagttca cgctgctgct gttcttcttc ttcacgtctt
    11821 tctttagtga caaccctgaa tttttcgagc atttcttcaa gaacgtcaat ggagaattcg
    11881 cgagccatcg cacggagagt gcgaatgtta tttaaacttt gtaacattac ggacataata
    11941 aagaaaacct tttaacgcca aaacaaaaaa gtatttcgtc gcgtagtgta agcgatcact
    12001 tattatattt ccacaaaatt taatctattt acagtcctgc gcgagattat ttcagctgtt
    12061 attttctgaa actctcagaa taatttcaag attatatatt agccattttg atga
//
LOCUS       DAZGAN020000140        11797 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGAN020000140 DAZGAN020000000
VERSION     DAZGAN020000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.140"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(59..2092)
                     /gene="betT"
                     /locus_tag="ACHKYO_RS21505"
                     /old_locus_tag="pgaptmp_004301"
     CDS             complement(59..2092)
                     /gene="betT"
                     /locus_tag="ACHKYO_RS21505"
                     /old_locus_tag="pgaptmp_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HGU4431038.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2221..2808
                     /gene="betI"
                     /locus_tag="ACHKYO_RS21510"
                     /old_locus_tag="pgaptmp_004302"
     CDS             2221..2808
                     /gene="betI"
                     /locus_tag="ACHKYO_RS21510"
                     /old_locus_tag="pgaptmp_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HGU4431039.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2822..4294
                     /gene="betB"
                     /locus_tag="ACHKYO_RS21515"
                     /old_locus_tag="pgaptmp_004303"
     CDS             2822..4294
                     /gene="betB"
                     /locus_tag="ACHKYO_RS21515"
                     /old_locus_tag="pgaptmp_004303"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HGU4431040.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQDAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4308..5978
                     /gene="betA"
                     /locus_tag="ACHKYO_RS21520"
                     /old_locus_tag="pgaptmp_004304"
     CDS             4308..5978
                     /gene="betA"
                     /locus_tag="ACHKYO_RS21520"
                     /old_locus_tag="pgaptmp_004304"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HGU4431041.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6191..6859
                     /locus_tag="ACHKYO_RS21525"
                     /old_locus_tag="pgaptmp_004305"
                     /pseudo
     CDS             6191..6859
                     /locus_tag="ACHKYO_RS21525"
                     /old_locus_tag="pgaptmp_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7102..7797)
                     /locus_tag="ACHKYO_RS21530"
                     /old_locus_tag="pgaptmp_004306"
     CDS             complement(7102..7797)
                     /locus_tag="ACHKYO_RS21530"
                     /old_locus_tag="pgaptmp_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HGU4431042.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7790..9217)
                     /locus_tag="ACHKYO_RS21535"
                     /old_locus_tag="pgaptmp_004307"
     CDS             complement(7790..9217)
                     /locus_tag="ACHKYO_RS21535"
                     /old_locus_tag="pgaptmp_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HGU4431043.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9228..9947)
                     /locus_tag="ACHKYO_RS21540"
                     /old_locus_tag="pgaptmp_004308"
     CDS             complement(9228..9947)
                     /locus_tag="ACHKYO_RS21540"
                     /old_locus_tag="pgaptmp_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HGU4431044.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(10474..11328)
                     /gene="rclR"
                     /locus_tag="ACHKYO_RS21545"
                     /old_locus_tag="pgaptmp_004309"
     CDS             complement(10474..11328)
                     /gene="rclR"
                     /locus_tag="ACHKYO_RS21545"
                     /old_locus_tag="pgaptmp_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HGU4431045.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            11554..>11797
                     /locus_tag="ACHKYO_RS21550"
                     /old_locus_tag="pgaptmp_004310"
     CDS             11554..>11797
                     /locus_tag="ACHKYO_RS21550"
                     /old_locus_tag="pgaptmp_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4431046.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNK"
BASE COUNT         3027 a         3146 c         2901 g         2723 t
ORIGIN      
        1 agcgagtcaa tgattcccgc gccatatgca gacatggcgc ggttttatgc aaaaacaatc
       61 acgcgtccgg gaacatcacg ctatggcccg gcgcttcacg atggagatga ataaagttaa
      121 ggtgccgctc gtactggtca agaatatcgg tgatcacctg ctctttgctg tagtccatca
      181 ggtcgttgcc ctggctgcct tctaacagga aggtttccag ccggtagtag gtcgatttac
      241 cgctacgtgc gcggtaggta aagcccggca ccgaatattg ctgcggccaa atctgataga
      301 caaagttttg ttcttcgccc atatgcacca acaaatccag atgccccaac tgttgtccct
      361 cttccggcgg caggcttttt agctccacgt acgcgccgcg caaccgcagc tcctgcgcca
      421 cttcttccat tgccgggtaa cagaccgtct ccatcatctg tttagtgtaa cgcgtgcccg
      481 gataattcat caggcgcgag agacgttttt tccagctcag gcgatcctga agccccagcg
      541 gtcgcggcgc ggtatcgcgg ttggcacttt cacggcggta atcttctacc ttcagagatt
      601 tatacaaccc cgccatcacg aagaagatca caaagctgaa cggcagcccc ataatcaccg
      661 tggtgttttg cagcgcggat atcccgttgg tcatcagcat gccgagcgtc agcaggccaa
      721 tcgccaccga ccagaagacg cgcagccagc cgggggcgtc gctgttgata tctttaagct
      781 gcgaggtgaa attccccagc accagcgcac cggagtccgc cgaggtcaca taaaacagca
      841 ggccagtaat ggtggcgacg gaggcgctaa aggtaaacgc cggatactgc gccagcaggc
      901 tgtagaagcc gcgctccgga tggaccatcg cttcctcggc aaatgccgcg ccgccgtgga
      961 tgatttcata cagcgcgcta ttgccgaaca ccgagagcca taacagcgtg aaggtaaacg
     1021 gaataatcaa cgtgcccagc acgaactggc gaatggtacg cccacgcgag atacgcgcca
     1081 ggaacaagcc gacaaacggc gaccatgcca cccaccatgc ccagaagaag agcgtccagt
     1141 tattcatcca ctcaactgga cggtcgaagg caaaactgtt gagcgtcatg cccataaagc
     1201 gattcacata gtcgccaaca ttcagcacca gcgcattaag caggaacgag gtgtcgccca
     1261 taaacaatac gaacaggatc aatcccagcg ccagcgcaac gttaagttcc gataacacac
     1321 gaatcccctt atcgacaccg gaggtgacag agatcgtggc gattatcacc gacaaggcga
     1381 tcagtgccgc ttttgccgcc atcgaatcgg gaatatcaaa cagtacgctc aagccatagt
     1441 taagctgcac cacaccgata ccgagcgtag tggcaatacc gaagatggtg ccgatcaccg
     1501 ctgcaatatc cactgagtgc cctatcggcc cgttaatccg tttaccgaag atcgggtaca
     1561 gcgccgagcg gatggtgagc ggcaaattat aacgatagct aaagtatccg agcgccatgc
     1621 ccatcagcgc atacatcgac cagccggtta agccgtagtg aaacagcgtc cagaccatcg
     1681 cctgacgcgc ggcctcaatc gtctgtcccg cgccttccgg cggctgcata tactgcgtta
     1741 ccggttcggc tacggagaag aacatcaggt cgataccgat cccggcagca aacagcatcg
     1801 ccgcccaact cagcaggctg aattccggtt tggattgttc tggcccgagc ttcaccgaac
     1861 caaaacgcga acaagcgata cagaccacaa agacaatata gagcgttgcc gccagtagat
     1921 agtaccaacc gaaggtttta gaaacccagt ccagcgtgcg gccaatccac agggccgaga
     1981 agtcgcgaaa caggatcgtt gtcagggaaa acaacaaaat cagtccggcg gaggtgtaaa
     2041 acaccaccgg attgattttg tccttttccc tgctgtgtga aaggtctgtc atccagtatc
     2101 cccactgtta ttgttaccat ttaaaatcaa attcgtaaca attaagacac attttatatt
     2161 gaacgtccaa tcaataaccg ctttaataga taaacaacgc tgatgaatgg agtggcgaaa
     2221 atgcccaaat tggggatgca gtcgatccgg cgcagacaac tgatcgacgc cacactggaa
     2281 gcaataaatg aagtgggcat gcacgatgca acgatcgcgc agatcgcccg ccgtgcaggc
     2341 gtttctacgg ggatcatcag ccactatttc agggacaaaa atggtctgct ggaagcaacc
     2401 atgcgcgata tcaccagtca gctgcgtgac gcggttttga atcgattaca tgcacttccg
     2461 cagggcagtg cagagcagcg attacaggcg attgttggcg gaaacttcga tgaaacgcag
     2521 gtgagcagtg cggcgatgaa agcctggctg gcgttctggg ccagcagtat gcatcagccg
     2581 atgctctatc gtttacagca ggtcagtagc cgccgcttat tgtcgaatct ggtgagcgaa
     2641 tttcgccgcg agttaccgcg cgaacaggca caggaagcgg gctacggcct tgccgcgctg
     2701 attgacggat tatggctgcg cgcggcactg agcggcaaac cgctggataa acctctcgca
     2761 cactcgctga cccgtcactt tattactcag catttaccca ccgattaacc gaggagacgt
     2821 gatgtcccga atggcagaac agcagcttta tatacatggt ggttatacct ccgccaccag
     2881 cggtcgcacc ttcgagacca ttaacccggc caacggtaac gtgctggcga ccgtgcagga
     2941 cgccgggcgc gaggatgtcg atcgcgcagt gaagagtgcc caacaggggc aaaaaatctg
     3001 ggcggcgatg accgcgatgg agcgctcgcg cattctgcgt cgggccgtcg atattctgcg
     3061 tgaacgcaat gacgaactcg caaaacttga aaccctcgac accggaaaag catattcgga
     3121 aacctcaacc gtcgatatcg ttaccggtgc ggacgtgctg gagtactacg ccgggctgat
     3181 cccggcgctg gaaggcagcc agatcccgtt gcgtgagacg tcatttgttt atacccgccg
     3241 cgaaccgctg ggtgtggtgg cggggattgg cgcatggaac tacccgatcc agattgccct
     3301 gtggaaatcc gccccggcgc tggcggcagg caacgcaatg attttcaaac cgagcgaagt
     3361 caccccgctt accgcgttaa agctggctga aatttacagc gaagcgggcc tgccggacgg
     3421 cgtatttaac gtgttgccgg gggtgggcgc ggagaccggg caatatctga ccgagcatcc
     3481 gggcattgcc aaagtgtcat ttaccggcgg tgtcgccagc ggcaaaaaag tgatggctaa
     3541 ctcggcggcc tcttccctga aagaagtgac catggaactg ggcggtaaat caccgctgat
     3601 cgttttcgac gatgcggatc tcgatctcgc cgccgatatc gccatgatgg cgaacttctt
     3661 cagctccggt caggtgtgta ccaacggcac ccgcgtcttc gttccggcga aatgcaaagc
     3721 cgcatttgag cagaaaattc tggcgcgcgt tgagcgcatt cgcgcgggcg acgttttcga
     3781 tccgcaaact aacttcggcc cgctggtcag cttcccgcat cgcgataacg tgctgcgcta
     3841 tatcgccaaa ggcaaagagg aaggcgcgcg cgtactgtgc ggcggcgatg tactgaaagg
     3901 cgatggcttc gataacggcg catgggttgc accgaccgtg ttcaccgatt gccgcgacga
     3961 aatgaccatc gtgcgcgaag agatcttcgg tccggtgatg tcgcttctca cttatgaatc
     4021 tgaggacgaa gtgattcgcc gcgccaatga taccgactac ggcctggcgg cgggcatcgt
     4081 gacggcggac ctgaaccgcg cgcatcgcgt cattcatcag ctggaagcgg gtatttgctg
     4141 gatcaacacc tggggcgaat ccccggcaga gatgcccgtt ggcggctaca aacactccgg
     4201 cattggtcgc gagaacggcg tgatgacgct ccagagttac acccaggtga agtccatcca
     4261 ggttgagatg gctaaattcc agtccatatt ctaaccagga ggtttatttg caatttgact
     4321 acatcattat tggtgccggc tcagccggca acgttctcgc tacccgtctg actgaagatc
     4381 cgaatacctc cgtgctgctg cttgaagcgg gcggcccgga ctatcgcttt gacttccgca
     4441 cccagatgcc cgcagcgctg gcgttcccac tacagggcaa acgctacaac tgggcctatg
     4501 aaacagagcc tgaaccgttt atgaataacc gccgcatgga gtgcggacgc ggtaaaggcc
     4561 tgggtggatc gtcgctgatc aacggcatgt gctacatccg tggcaatgcg ctggatctcg
     4621 ataactgggc gcaagaaccc ggtctggaga actggagtta tctcgactgc ctgccctact
     4681 accgcaaggc cgagactcgc gatgtgggcg agaacgacta ccacggtggc gatggcccgg
     4741 tgagcgtcac cacctccaaa cccggcgtca atccgctgtt tgaagcgatg attgaagcgg
     4801 gcgtgcaggc aggctacccg cgcacggacg atctcaacgg ctatcagcag gaaggttttg
     4861 gcccgatgga tcgcaccgtc acgccgcagg gccgtcgcgc cagtaccgcg cgcggttatc
     4921 tcgatcaggc caaatcgcgc cctaacctga ccattcgtac tcacgctatg accgatcaca
     4981 tcatttttga cggcaaacgc gcggtgggcg tcgaatggct ggaaggcgac agcaccatcc
     5041 caacccgcgc aacggccaac aaagaagtgc tgttatgtgc aggcgcgatt gcctcaccgc
     5101 agatcctgca acgctccggc gtcggcaacg ctgaactgct ggcggagttt gatattccgc
     5161 tggtgcatga attacccggc gtcggcgaaa atcttcagga tcatctggag atgtatctgc
     5221 aatatgagtg caaagaaccg gtttccctct accctgccct gcagtggtgg aaccaaccga
     5281 aaatcggtgc ggagtggctg tttggcggca ctggcgttgg tgccagcaac cactttgaag
     5341 caggtggatt tattcgcagc cgtgaggaat ttgcgtggcc gaatattcag taccatttcc
     5401 tgccagtagc gattaactat aacggctcga atgcagtgaa agagcacggt ttccagtgcc
     5461 acgtcggctc aatgcgctcg ccaagccgtg ggcatgtgcg gattaaatcc cgcgacccgc
     5521 accagcatcc ggcgattctg tttaactaca tgtcgcacga gcaggactgg caggagttcc
     5581 gcgacgcaat tcgcatcacc cgcgagatca tgcatcaacc cgcgctggat cagtatcgtg
     5641 gccgcgaaat cagccccggc acggaatgtc agacggatga acagctcgat gagttcgtgc
     5701 gtaaccacgc cgaaaccgcc ttccatccgt gcggtacctg caaaatgggt tacgacgaga
     5761 tgtccgtggt tgacggcgaa ggccgcgtac acgggttaga aggcctgcgt gtggtggatg
     5821 cgtcgattat gccgcagatt atcaccggga atttgaacgc cacgacaatt atgattggcg
     5881 agaaaatagc ggatatgatt cgtggacagg aagcgctgcc gaggagcacg gcgggatatt
     5941 ttgtggcaaa tgggatgccg gtgagagcga aaaaatgagt cgtgatgtga actaacgcag
     6001 gaaccgatca gattgagagt taccgttcca gagaggggga ccgaatcctt atataaacac
     6061 tgaggtaact ctcatgcatc atatccagta ttcatgatgc tggctttttg ctgatattta
     6121 acccgaacat tcctgataca gactattaat acaaccttat ctgcgacaga ttttattttc
     6181 tggatatatt atgcgtgaac aaatcaaaca ggatatcgat ctgattgaga ttttatttta
     6241 tctgaagaaa aaggttcgtg ttatcctttt tattatagct atctgtatgg caatgatgct
     6301 gttgtttctg tatatcaata aagataatat aaaagtgagt tacagcctaa aaataaacca
     6361 gacaacacca agcatacttg ttaactgtga tagcaataat aactttgcct gtcagaccgc
     6421 aatgactgaa gatgttatac agcgaattac tacatttttt cacaccagcc cagatgtcaa
     6481 gaacagagaa ataaagctgg aatggtcatg agataagaga gatttaccaa ctgctgaagc
     6541 ggaaatatct cgcgtgcagg cgtctattat caaatggtat gcgtcagaat accataatgg
     6601 caggcaagtt ctcgatgaga tacaaatacc ttcagcaatt aacagtgagc tttatacaaa
     6661 aatgatatac ctgaccagga actggtcatt gtatccgaac ggtgatggct gtgtaactat
     6721 aagctcacca gaaataaaaa ataaataccc tgctgccatt tgcctggctc tgggattttt
     6781 tctaagcatt gtaatttctg taatgttttg ccttgtcaaa aaaatggtag atgaatacca
     6841 acaaaactct gggcagtaat acacagcatt ctgatagaga gtgcctgtat aaagctgaag
     6901 tgacaatgac cgggaaggga acccgttttc aggaatggat gcctgtcttt ttcactgtaa
     6961 atatcctggc attactaatg ccaggatatt tacagaagag cagcataacc atgacggaac
     7021 ggggttaagt caggttgtcc ttaacgctat tttcaacctc gtatgccttc ttcaggttta
     7081 tgttcagatc tcatatctct ctcagcaatc ctcaataatc agatacaccg ctttcaccgg
     7141 gccatgaacc ccgacgactt tgataagctc aatatccgcc gttgaactag ggccgctaat
     7201 gatgttaatg caggacggca ttcgttcacc ggcctgcgct ttctgatgca atttttctgc
     7261 gagttgcgct acacgcggca ggattgtgct tttacgcagg ataaaaagag aagattccgg
     7321 gagcaggctc aatgaacgcc cgcgcccggc ggcggaaaaa agaaccacgc ctcccgattc
     7381 ggttaaacca tattcagcat acacgacacc cactttagcc tgctcggcct gcgagatatt
     7441 ctcggcacct ttcgccggat cccaaacaac ggcattgcat tcctgctgca aacgttcact
     7501 aatccccaat tcctccagcc tcgtgtcacc gctaatcacg accgactgat ctcccagctc
     7561 tttacacaga cgtattgcag cttctgccgc cttcgcctcg ctggtcagct cacagcgcgt
     7621 caacataaca tcgctggcaa actgaataaa cgcgtcacag cgctgctgtt ggttaagttg
     7681 ggtaagccgc tcgttagcat agttgttaag cggcgcatct tctgcttgcg gttcaagtcg
     7741 cagcgggcga cccagtgcct gagcaacgtt attcaaaaat tcgcctcgat tatccattct
     7801 ttttctcctg cgcctgatgt ttcttaaacc aactacggaa actctctccg tcagcttcag
     7861 gaagatcgcg tgcttccatc cagtcgctaa tcgcgccaaa tttgagtggt gttttgccgc
     7921 cattgataaa ccagcttgcc gcatgagcgc cggccatcat cccgactttc cacaatcctg
     7981 gatgactatt ggcataagcg aacattttta tcgcccgttg ctctgctttt gcggtgatcc
     8041 ctttttcagc catcacccga cgatgacgca aaatcagttt tgacagcgga atacgcaccg
     8101 gacacacgct gtcacaagca gtgcataaag agcaggcgta gggtaaatct ttaaaatctt
     8161 tatagccgcc aagtagcgga gaaatcaccg caccaattgg ccctggataa atagagccat
     8221 atccatgacc gccaatatgg cgatatgccg gacaagtatt catacaagcc ccgcagcgaa
     8281 tacagcgcag cacatcccga aattcagagg ccagcacctc agaacgcccg ttatcgacaa
     8341 taaccagatg aaactcttca ggaccatcaa cgtgccccgc ttcgcgcggt cctgtcagcc
     8401 aggtgttgta tcccgtcaaa cgtgcaccaa cggcactgcg cgccagcatg gtgatcaata
     8461 catctacctc ggcaaacgtg ggggcaatac gctccattcc catcactgca atatgcgttt
     8521 taggcagcgt ggtacacatt cgcgcattac cttcattggt caccaggcat accgaacctg
     8581 tctctgccac cgcgaaatta cagccggtaa tacctatttc agcactgagg aaatcttcgc
     8641 ggattttttg ccggatgaat aaggtcatcg cttcaggcgt ttccggcccc tcatagccca
     8701 gacgttcgtg tagcactcga cggatctgat ggcgatcttt atgaattgcc gggaccacaa
     8761 catgagatgg cggatcttga tccagctgga gaatatattc acccagatcg gtttcaatca
     8821 cctgaatgcc agcatcctgc aacacatgat tgaccccaat ctcttcggtc accatcgatt
     8881 tagatttcac caccttccgg gcatttttgc gttgggcaac ctgtaaaatg tagcgggtag
     8941 cgtcttcttt ggtttttgca aaccagacgt gaccgccgtt ttgcgtcact ttttctgaga
     9001 gctggtacag ataagcgtcg agattactca gaacatgatc acgtatctgg gcggcccgat
     9061 cgcgccactc ctcccagtgc cccaattcat cgaccatttt ttgccgattt gccccaatac
     9121 gctgctgcgc gtttgccacc gctttgcgca tgatcggatc ttcaatttgc tgacggatgc
     9181 gtgtcttaaa atctgtatta ctggttttga tcgacatctt tatatcctca gcggctcatc
     9241 aacacttcag caatatgcat cactttgact ttctgccctt cccgttgtaa tcgcccactg
     9301 atgttcagca ggcaactcac gtcagcacca attaaatact cagggcggac ttccatcagg
     9361 tgcacaacct tttctttcac catctcgccg gatatttcgg ccattttgac cgagaacgtg
     9421 ccgccaaatc cgcagcaggt atcctgttca gcaaaggtca acagctccag tccacgcaca
     9481 tttttcagca gcgtaagtgg ctcgtccttc actcccagct tacgggccag gctacaagat
     9541 gggtgataca cagctctccc ttgcaaactg gcacctacat cgactacccc taatttatta
     9601 acaataaaag aggtgagatc ctgcatacgc gcggcaacct tttcggcacg tgatgcccat
     9661 tcaggttcat ccgccagata cgtcgggtaa ctttttacgg cataggtgca agagccagcc
     9721 ggtgaaataa tgggatcgtc gttatcctcc agtgcggcga tcagattttt catccctgga
     9781 atcgcttctt tgatataacc gctattgatc gcaggctgac cgcagcatcc ctgtttctcc
     9841 gggaaattta cgcgacagcc gagtttttcc agtagcagca cggagtctcg tgccattctt
     9901 gatttcaggg cgtcaccaat acaggtgaca aagaaattga cattcacaac taatactcca
     9961 ttacttcatg cccatttatg cccgatgttt cccccccacc gggggatcaa tcatcaataa
    10021 atatggcgtt cttttttaat tggctagcaa cataccaccc ctcatttgta tgacaaatga
    10081 taatctttca tccaaacttc aaatgcaata actaatttta tttctttcgg catcgctcac
    10141 attttatggg gaaatcgacg ttcatttttg gcataagaaa tccatttaaa aaggctacat
    10201 cccttatacc tattcagagc tacttattca ggggttattg agatatacac tgatggtgta
    10261 caaaaaaatc accgacaata atcacgtatc aagtaatagt cggggtttta tttaattgaa
    10321 gcaatttagt ttgcattttt atttaaaaac tgagctagag tccatcagaa aaaaatatat
    10381 aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg ctcacaactc ccggaatgcg
    10441 gattatcttt gccctgattt ctggcggttt tattcagggt gcaagctgtc tgaccctttc
    10501 ccgatattct cccggagtac aaccaaactc gcggacaaac gccttgtgaa aagatgattc
    10561 actggcatag cctactgact cagcgatcac cacaacaggg agcatttccc gggaaaacat
    10621 ctgggccgct atttgtagac gcaactttgt taatacagcc agcggcgtgg ttccggaaac
    10681 atcacggaaa agctgggcaa aacttgcccg ggacatgtga gcgatgctgg ccagcgattc
    10741 gacggtccag gcgtgtcctg gcatttccag catttgctgt attaccgcac caagacgtgg
    10801 atgcagaagc aaactgagaa tgtttttctc agtattaacc tgtgcaatcc actcacgcac
    10861 cgcgagggta aagaatgtag cgcagatctg gctacacagt gcatccactc ccggcatcgc
    10921 cgatctggat tcctgttgta aaaacgggat cgcctccctc agccagttat attccacgct
    10981 gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac gttaaaaaat aacgcgccga
    11041 atgttgcaac cgaagagtgc cgcagacaat acaggtcgat tcattatcga catgactcag
    11101 gcgatgagct gaattttgtg gtagcaggac cacatttccc gggcgtaatg taaaaatctc
    11161 tcccgtcggc atttccagct tcgccgctcc ttgcgttaac gcatgccaac gaataaccga
    11221 taattccccg gcaccatgtg gaagctgcca gtcacttcct aacacgcaat tcttatcgat
    11281 cgttccttgt ggagcgttaa gcatcaacaa acggctaagg gcatccatat cagactcctg
    11341 agtggttttt gattacaaac accaacaatc ttagtaaata actaatctaa taccatcaaa
    11401 tggttaatca tgatttttgt tttgtgattc tgattaagaa aaagaaaata ccagacgata
    11461 agacaaaata tcaagcgttg tgaagaaatg gtatttgctc ttttgcgtct atagtcatga
    11521 tgtcaaatga acgcgtttcg acaggaaatc ataatgaata aatatcaggc agttattatt
    11581 ggttttggca aggctggaaa aacattagcc gtcacgctgg caaaagcagg ttggcgagtg
    11641 gctctcattg aacaatcaaa tgcaatgtat ggcgggacct gtattaatat cggctgcatc
    11701 ccaaccaaaa cattggttca tgacgcacag cagcacacag attttgtccg tgccatacag
    11761 cgtaaaaatg aagtggttaa ttttttacgt aataaga
//
LOCUS       DAZGAN020000141        11511 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGAN020000141 DAZGAN020000000
VERSION     DAZGAN020000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.141"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..96
                     /locus_tag="ACHKYO_RS21555"
                     /old_locus_tag="pgaptmp_004311"
     CDS             <1..96
                     /locus_tag="ACHKYO_RS21555"
                     /old_locus_tag="pgaptmp_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431047.1"
                     /translation="EAEAEAEAEAGQDAPRKKTGNKAKQAKKARA"
     gene            93..512
                     /locus_tag="ACHKYO_RS21560"
                     /old_locus_tag="pgaptmp_004312"
     CDS             93..512
                     /locus_tag="ACHKYO_RS21560"
                     /old_locus_tag="pgaptmp_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HGU4431048.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLAVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            509..1069
                     /locus_tag="ACHKYO_RS21565"
                     /old_locus_tag="pgaptmp_004313"
     CDS             509..1069
                     /locus_tag="ACHKYO_RS21565"
                     /old_locus_tag="pgaptmp_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HGU4431049.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIVVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            1070..1315
                     /locus_tag="ACHKYO_RS21570"
                     /old_locus_tag="pgaptmp_004314"
     CDS             1070..1315
                     /locus_tag="ACHKYO_RS21570"
                     /old_locus_tag="pgaptmp_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HGU4431050.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            1312..2814
                     /locus_tag="ACHKYO_RS21575"
                     /old_locus_tag="pgaptmp_004315"
     CDS             1312..2814
                     /locus_tag="ACHKYO_RS21575"
                     /old_locus_tag="pgaptmp_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGU4431051.1"
                     /translation="MINFDGIGNDRRIPLIGVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGTGVLDVPVRITRASQAVELWGRGSMIALMVAEFIAINPDAELYAIAQGAGT
                     GGANAAAFNLTGTAATDGLLCIYIGGRRYALPVKAGQKGKAIIDALVALINKDTDAPF
                     TAVSAVVDGSNAGGLKGSMGVSARFIGECSVHDLRLNYHDGETLPSGLTPQTGFPSSK
                     ARNPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPDKMSDGVLWLAHTG
                     TFGEVQAFGAKRNDFLATCTSIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALP
                     SRMAPAPADRLTREERNSLLWGGIATVSVAAGDMVQIERQVTMYRQNVYGESDPSYLD
                     VETIYTLSYLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     ELGLVENLDTFKKNLLVERNTSDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            2823..3188
                     /locus_tag="ACHKYO_RS21580"
                     /old_locus_tag="pgaptmp_004316"
     CDS             2823..3188
                     /locus_tag="ACHKYO_RS21580"
                     /old_locus_tag="pgaptmp_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGU4431052.1"
                     /translation="MSGKQYQGTATIRVNGQEYDTLEGATFSPSGYEREVVKGAKVYG
                     YREKPREATLDCKFPAGGEGSPAADEINTWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISAKFASATSTRVQ"
     gene            3203..3682
                     /locus_tag="ACHKYO_RS21585"
                     /old_locus_tag="pgaptmp_004317"
     CDS             3203..3682
                     /locus_tag="ACHKYO_RS21585"
                     /old_locus_tag="pgaptmp_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HGU4431053.1"
                     /translation="MSIRKKKTALSDEAVMEAIRDALEDGDPRTAGLAEQLANGYVDL
                     LDGLPFGESREYRVTFRELTAKDSIDAETEAERLMETRNGGPVLVASPALRGIALLRR
                     QIAAVGQIEGPLSPRQIGQLSERDLSRLMAAVSLLDSAMAGKLVAERGRPDAVPGSD"
     gene            3810..5867
                     /locus_tag="ACHKYO_RS21590"
                     /old_locus_tag="pgaptmp_004318"
     CDS             3810..5867
                     /locus_tag="ACHKYO_RS21590"
                     /old_locus_tag="pgaptmp_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4431054.1"
                     /translation="MAKNLKASLIVDLLGNISAKSRQWSQELGAFSRSGRTGLGGLGN
                     AARRAGQETELVGSRIQRTLAGVRGSIRTVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNKAFIRPAAEMENYILRLNAINHGDTAKTEAVKAWAVQNARDTTWGLAGVMQ
                     EYASSRGFGMSDREARRFITMLQDQGGYHGWSLSDAQGASLQLKQMFARQSIQAADAS
                     ILTGYGINVYQLLADKLGVNQKIIREKGEKGKLGPDSIRLLFQVMAEQAKGAQKNAMN
                     SWTGMTSMMGDVWDQFAREVMAKGPFDSLKKSLKGFLDYADAAQKSGLQDKLATQTAS
                     VLNQGFEYARDAATGFYRAIQKVRETLQALRDAGYGDALDRIGHGAQTAAKYLLYMYL
                     ATRALKAVRAIGLGALRPGAALLGYGLSTAAVLTSPFRKPQTTVPGTQPGLGQRFVNF
                     LTGVNPAAVQPVLVTNWPAGGLASGGGDVVVSGDGKTVRGRKKRGPGRGRGVTTVVTA
                     GEQLAESAGKQGFFGRMMSRAGGLLSAAGNRMGLGRFAGLFRGAGRLGGGALWAGAMA
                     APVLLDSSASAADKAGAVGSLAGSIAGGALGAAAGPVGVAIGSTVGSYLGDYLGGWLT
                     QAWQKLRGSSDENGGQATAKTAARVELVAPEGWRARSIDVDDTAQHGLDVNVWNGGNY
                     GLY"
     gene            5854..7212
                     /locus_tag="ACHKYO_RS21595"
                     /old_locus_tag="pgaptmp_004319"
     CDS             5854..7212
                     /locus_tag="ACHKYO_RS21595"
                     /old_locus_tag="pgaptmp_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HGU4431055.1"
                     /translation="MASTDTPGRGSFRGVPFLVYQEQRERGGRNIVRREYPLRESGGA
                     DDLGPKLPEFTFTVLVTGDDLQTQRSRLRDALRAPGAGELMHPDYGTLNVLINSFESR
                     YNASEQGTVEFTINVIPASDDTAPSVAEDTAAALEQKSGSAMNQLFNTLSDGWTVISD
                     GLHDVQAMTDTISDKIDALENAVSGMGIVQDVSAFTASFTALKGNAAALINAPRRMAE
                     SLAGIFAVLTGLPGNPSLSLTGKAGRPSGSLTTNRDSMTEQAMPQLYRTLSSLRYTFS
                     EQDDPQRLIGLTPAAQKNIRLLRSVMQSATLVSQAQTVGKLLDQVIRQNTTHVQDTDR
                     AGGLAWLESSADVQRINRDLSDAMEQQVLDLSAQGHTGTALALRDAGLALTEDLTTRS
                     VHLPGAFQVMVRTTEPALVTLYRATGNSRRWQYFVRRNNIPDPVFVPGGRSVEVISEQ
                     QD"
     gene            7196..8320
                     /locus_tag="ACHKYO_RS21600"
                     /old_locus_tag="pgaptmp_004320"
     CDS             7196..8320
                     /locus_tag="ACHKYO_RS21600"
                     /old_locus_tag="pgaptmp_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HGU4431056.1"
                     /translation="MSSRIELYIGGSIFSGWLTVSVRRSLEHLAGSFELGLMLPGERI
                     PSALRTGQSLTLRINGQTVISGWLDQVSQRISATRHQISISGRDKTGDLVDCAAIHPG
                     SQWRNRTLAQIAADLCAPFGIAVRWQVNDDTAARPFSSFTLENSETVADALTRAARHR
                     GVLVTSNADGDLVFTQAGSQQTDRLVLGDNLLDADYNTDWRGRYSEYRVRGHGRGGGK
                     RGDSESAARLAAPVGVISDEQIGRYRPKIILADQQTDATGARQRALREMRRAIARSER
                     FSATVRGWFRDDGRLWDVNLLTGVSALRFGIEQTELLVCQVEFLLDEQNGEVTRLVLA
                     PRDGFIVPAEPDSKGRGGSGDDVDAFIRQQMKKQGISFDE"
     gene            8310..8924
                     /locus_tag="ACHKYO_RS21605"
                     /old_locus_tag="pgaptmp_004321"
     CDS             8310..8924
                     /locus_tag="ACHKYO_RS21605"
                     /old_locus_tag="pgaptmp_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU4431057.1"
                     /translation="MMNDEVFSRLIAPVTRGIRLLFGRGVLTGTHDELKMQNVQLTGM
                     DGETFDDVERPLQYGQISVPLPGAETFFACLGGQRDQTVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKDGRVIVTCKMLEIYADEGMRVDTPEATFTGNVTVDKNLHIKGNL
                     TIDGTGKSQGTFTMSEAVIAGITYSGHVHQDNGKGSKTGAPENG"
     gene            8917..9354
                     /locus_tag="ACHKYO_RS21610"
                     /old_locus_tag="pgaptmp_004322"
     CDS             8917..9354
                     /locus_tag="ACHKYO_RS21610"
                     /old_locus_tag="pgaptmp_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HGU4431058.1"
                     /translation="MADIAIVWDQGCGSLQLNGADLLTDNSLLTAVLISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPVGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHDRLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            9354..10436
                     /locus_tag="ACHKYO_RS21615"
                     /old_locus_tag="pgaptmp_004323"
     CDS             9354..10436
                     /locus_tag="ACHKYO_RS21615"
                     /old_locus_tag="pgaptmp_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGU4431059.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYAQAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNAPPGTKLTFITPQAGINQ
                     TTTVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCRP
                     EWPQAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FDVRSPLKSVMAGDTELLTVGEITWL"
     gene            10427..10984
                     /locus_tag="ACHKYO_RS21620"
                     /old_locus_tag="pgaptmp_004324"
     CDS             10427..10984
                     /locus_tag="ACHKYO_RS21620"
                     /old_locus_tag="pgaptmp_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HGU4431060.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWIGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPESQWISIVTVKTAVGYRHMNVLDDILTPLRIYDASALE
                     CILNRYKPAWQTFLYLYENSHEETE"
     gene            10984..>11511
                     /locus_tag="ACHKYO_RS21625"
                     /old_locus_tag="pgaptmp_004325"
     CDS             10984..>11511
                     /locus_tag="ACHKYO_RS21625"
                     /old_locus_tag="pgaptmp_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431061.1"
                     /translation="MYFVDNNSGVTDMPPLAPSQGTQVKWFTEGDGRKGISYIGQDWL
                     NIVQAELLAILTEGKVQPDKAKLNQLVTAIKAIIAANAYSRKSNLKEIADAGAEAQAA
                     ARRHLGLGGLSGKDSLAANDVGALEKSMNFGDVPDKTSARQNLDVYSKSEGDNRYLRK
                     DQNGADIADKGTFIDN"
BASE COUNT         2532 a         3024 c         3594 g         2361 t
ORIGIN      
        1 gaagccgaag ccgaagccga agccgaagcc ggacaggatg ctccccggaa aaagaccggt
       61 aacaaagcga aacaggcgaa aaaggcccgc gcatgaatta cgccactgaa accgatatgc
      121 gggcgcgtta ccgcgaggat ttgctcaggc cgttactggc agtgccccgt tcggatgagc
      181 cggacacgcg caagctgaac cgggcgctga cagatgcgtc agcccttatc gacagctatc
      241 tgtccgcccg ttacacgctg ccgctggcgg ttatcccggc tgttcttgtt cagcactgtt
      301 gcgcgattgc cttttattac ctgtgcgatc agcgagcctc cgatcaggcg cgtgaccgtt
      361 accgtgaagc gctggcctgg ctgaaagatg tcatgaacgg caatgtgccg gtcggtgtgg
      421 atacgaacgg tgcggcccct gaatccgggg atttaccgca ggttcagtct gatgcagcgg
      481 tattcgggcg caaccagaag ggcttcatat gattacggaa accgaacagg catacatcgc
      541 ccgtatccgt gagtatttcg ggaatgaact ggtgtctgtt gacacgcatc ccggcgactg
      601 gagcgacagc gtactgcgca ccatgctgat taacgccccg gcgatctacg ttgcctggct
      661 gggtgccggt gaaggccgta cccgtggtcg cctggtcagt cactgggtgt tctacgtcat
      721 cggcgacatg ctcaacgggc gtgaggccag ccgtcccgga ctgtatcaga ttgtggcccg
      781 gctgattgtc gtgcttaacg gcttcagaac agaaaaaacc tcaccgcttt actttgaaaa
      841 ggcggtcaac ggttacaccg aaacccaggc ggacagtggt gcggtgatgt atgcgcttta
      901 tttctcctgc gaggaaatga tcgcaccgct gaccgatatc agctcgctgg acgacttcct
      961 gcgtcattac gaaacctttg tggagccgcc cggtacgccg ccgtttgagg cgcatattga
     1021 tttgccgggg aacacgacaa cggcggtcga accgccggag gaactgtaaa tgaaaaccat
     1081 ctttatcaaa cccgcgccgg gacgcctgat tcgagatccc gacacaatgc gcccgctggc
     1141 acaggagggg gaggaaaagc ccttcacgcc gttctggtgt cggcgtcttg atgacggcga
     1201 tgtcattcag gctgaaaaag ccgctgaaga ggctccggct gtatctgcgg atgcaacgac
     1261 tgcgacagaa aaaccggtcg cccaaccggc atccgataag gagaaaccgc aatgattaat
     1321 tttgacggga tcggtaatga ccgccgtata ccgctgattg gggttgagtt taataactcc
     1381 atggcggtca ccggcacccc ggcacagcgt cagtgtgtgc tgctgtttgg tcaggcgaga
     1441 atgaaagaga atgctgtgga cggtaccggt gtgctggatg ttccggtcag gatcactcgc
     1501 gcctctcagg ctgtggaact ctgggggcgt ggctccatga tcgctctgat ggtggctgaa
     1561 tttattgcca tcaatcctga tgcggaactg tatgctattg cgcagggggc cggaacaggc
     1621 ggggcaaatg ccgccgcctt taaccttacc ggtacggctg cgaccgatgg tttgctctgc
     1681 atctatattg gtggacgacg ctatgcgctg cccgtaaaag ccggacaaaa aggaaaagct
     1741 atcattgatg cgcttgttgc cctgataaat aaggatactg atgcgccatt cacggctgta
     1801 tctgcggttg ttgatggcag taatgcaggc ggtctgaaag gaagtatggg agtgagcgcc
     1861 cgcttcattg gtgaatgctc tgtgcacgat ctgcgcctga attatcacga cggtgaaacc
     1921 ctgccgtcag ggctgacgcc ccagacaggc tttccctcct caaaagcccg caatcccgat
     1981 atcacccgaa gtgtggccgg aatgggggat cgccagtaca actatgttgt catgccctat
     2041 aaggacgatg caaacctgaa aatcatcagt gatgaacttc tgaaacgctg gggaccggac
     2101 aaaatgtctg acggcgtgct gtggctggca cataccggca cgtttggtga ggtgcaggca
     2161 ttcggggcaa aacgcaatga ttttctggcg acctgcacat ccattcctaa agctcctgaa
     2221 ccggattacc tctgggcagc ggcggtctgc gccacctgtg cgccgtctct gggggcagat
     2281 cctgcgcgtc cgcttcagac gctggcgctt ccgtcacgca tggccccggc tcctgccgat
     2341 cgtctgacgc gtgaagagcg taacagtctg ctgtggggag ggattgccac ggtcagtgtg
     2401 gcggccgggg atatggttca gattgagcgt caggtgacga tgtatcgcca gaacgtgtac
     2461 ggcgaaagtg atcccagcta tctggatgtg gaaaccatct acaccctgtc atatctgcgt
     2521 tattccctgc gcactttcat cactcaacgt tttccgcgcc ataaactggc tgatgacggg
     2581 acgcccgttc gtgccggtca gaatatcgtg acgccggaga tcatgaagct tcagttaatt
     2641 tcgctgggtg aagagtgggt ggaactcggg ctggtcgaaa atctggacac gtttaaaaag
     2701 aatctgcttg tggagcgtaa tacgtcagat cgaaaccgcc ttgatgtgtt atgcacaccg
     2761 gatctggtta accagttccg cttcctggca gcacagatcc gtttcatttt gtgaggtaag
     2821 gcatgagcgg aaaacagtat cagggcacgg ccaccatccg tgtgaacgga caggagtacg
     2881 acacccttga gggggccacg ttctccccgt ccggctatga gcgtgaagtg gtgaaaggcg
     2941 cgaaagtcta tggctatcgc gagaaacctc gtgaagcgac gctggactgc aaatttccgg
     3001 cgggcggaga aggttcaccg gctgccgatg aaatcaacac ctggactgcg gtcacgattg
     3061 agtttgtggc tgataccggt gaagtccata tgatgacgaa ggcctggagc agtgaaccgg
     3121 cctcgcttga cggtggcggg gagatctccg cgaagttcgc cagcgccacc agtacccgtg
     3181 tccagtaatc aggaaaaatg caatgagcat acgtaagaaa aaaacggcgc tttctgacga
     3241 ggccgtgatg gaggctatcc gggatgcact tgaggacggc gatccccgca ccgccgggct
     3301 ggctgaacag ctggcgaacg gctatgtgga tctgctcgac ggcctgccgt tcggggaatc
     3361 ccgtgaatac cgcgtcactt tccgcgaact gacggcaaaa gacagtattg acgcggagac
     3421 cgaggccgaa cgtcttatgg aaaccagaaa tggcggcccg gttctggtgg cctcaccggc
     3481 actgcgcggc attgcactgt tgcgccgtca gattgccgct gtcggccaga ttgaggggcc
     3541 attgtctccg cgtcagatcg gacagctcag tgagcgcgat ctctcccgcc tgatggcggc
     3601 ggtaagtctg cttgattcag caatggccgg aaagctggtc gctgaacggg ggcgaccaga
     3661 tgcagtgccg ggatcagatt gaagaagcgg caattgtgct ggggatggtg acaaaaagcg
     3721 gcccggagtg ggcgcttaac ctccccttat cgcagcttta ccggcactgc cgacagaccg
     3781 aaaaaattct cagaacgaag cagtaaccca tggcaaaaaa cctgaaagcc tccctgattg
     3841 tcgatttact cggcaatatt tccgccaaat cccgccagtg gtcacaggag ctgggggcgt
     3901 tctcccgttc cggcaggacg gggctgggtg gtcttggaaa tgcagcccgc cgggccggtc
     3961 aggagacaga gcttgtcggc tcccgtatac agcgaacgct tgccggtgtt cgtggcagta
     4021 tccgcacggt gacgtcagat tttgaccgcc tgcagggcag cattaccggc accatcgggc
     4081 ggataagcaa cctttacggg atgctggcgg gaggggctgc ggtctacggt tttaataaag
     4141 cctttatccg cccggccgca gagatggaga actatattct gcgtctcaac gccattaatc
     4201 atggcgatac ggcaaaaacg gaagcagtga aagcctgggc ggtacagaac gccagagaca
     4261 ccacctgggg gctggcgggg gttatgcagg aatacgcctc cagtcgcggg ttcggtatga
     4321 gtgacaggga agcccgtcgc tttattacca tgcttcagga tcagggcggc tatcacggct
     4381 ggtcactgtc agatgcacag ggtgcgtccc tgcaactcaa acaaatgttt gcccgccaga
     4441 gtatccaggc agcggacgcc agtattctga ccggatacgg gattaacgtt taccagttgc
     4501 tggccgataa gctgggtgta aaccagaaga tcatccgtga gaaaggcgag aaaggaaaac
     4561 tgggaccgga cagcattcgc ctgttatttc aggtgatggc cgaacaggca aaaggtgccc
     4621 agaaaaacgc catgaactca tggacgggaa tgacgtccat gatgggtgac gtctgggatc
     4681 agtttgcccg cgaggtgatg gcaaaggggc cgtttgacag cctgaaaaag agcctgaaag
     4741 gcttcctgga ttatgctgat gcggcacaga aaagtggatt acaggataag ctggccacac
     4801 agacagcctc agtcctgaat cagggatttg agtatgccag agacgctgcg accggctttt
     4861 accgggccat tcagaaagtc agggaaacgc ttcaggcact gcgcgacgcc ggttatggcg
     4921 atgcgctgga tcgcattggt catggcgcgc agacagcggc gaaatatctc ctgtacatgt
     4981 atctcgccac ccgtgcactg aaggcagtcc gcgcaatagg actgggagca ctgcgaccag
     5041 gagcggcatt gttggggtac ggattatcca cggcggccgt gctgacttca cccttccgca
     5101 aaccgcagac cactgtgccg ggaacgcagc caggactcgg ccagcgtttt gtgaactttc
     5161 ttaccggggt aaatcctgct gccgttcagc ccgtgctggt caccaactgg cctgcgggtg
     5221 gactggcatc aggtggcggg gatgtggtgg tcagcggtga cggcaaaacc gtgcgcggcc
     5281 gtaaaaaacg cggccccgga cgcgggcggg gtgtcacgac cgtcgtgacc gccggtgagc
     5341 agctggcgga gagcgccggt aaacagggct tcttcggtcg tatgatgagc cgtgccggtg
     5401 gtctgctttc ggctgccgga aaccgcatgg ggctggggcg ttttgccggg ctgttccgtg
     5461 gtgccggtcg gctgggtggt ggtgccctgt gggccggtgc catggcggct cctgtactgc
     5521 tggacagcag cgccagcgca gccgataaag ccggtgccgt gggttccctt gccggaagta
     5581 tcgccggtgg cgcgctggga gcggctgccg gtccggtcgg ggttgccatt ggttccacgg
     5641 tgggcagcta tctcggtgat tatctggggg gctggctgac gcaggcctgg cagaaactgc
     5701 gtggcagcag tgacgaaaac ggcggacagg ccaccgcgaa aactgccgca cgggtggagc
     5761 ttgtggcccc tgaaggctgg cgggcgcgca gtattgatgt ggatgatacc gcacagcatg
     5821 gcctggatgt gaacgtctgg aacggaggga actatggcct ctactgacac gccggggcgt
     5881 ggctcctttc gtggcgtacc gttcctggtt tatcaggagc agcgggaacg gggcggacgt
     5941 aacattgtgc gccgtgaata tcctctgcgg gaaagcggcg gcgcggatga tttggggcca
     6001 aaactcccgg agttcacatt cacggttctg gtgaccggag acgatcttca gacacagcga
     6061 agccgcctgc gtgatgcgct gcgtgcaccc ggtgccggtg aactgatgca cccggattac
     6121 ggcacgttaa acgtgctgat aaacagcttt gaaagccgtt ataacgccag cgagcaggga
     6181 acggttgagt tcacgatcaa cgtgatcccc gccagtgacg ataccgcgcc ttcagtggca
     6241 gaagatacgg cagctgcgct ggagcagaaa agcggttcag caatgaatca gctgtttaac
     6301 acgctttcag acggctggac ggtgatttca gacggtctgc atgatgtaca ggccatgacc
     6361 gacaccatca gcgataagat tgatgcgctg gagaatgccg tttcagggat ggggattgtg
     6421 caggatgtca gtgcctttac ggccagtttt accgccctga aaggcaatgc cgccgccctg
     6481 attaatgcgc cccgtcgcat ggctgaatct ctggccggaa tatttgctgt gctgaccgga
     6541 ctgcccggca atccctccct gtcactgacc gggaaggcag gaaggccgtc cggcagcctg
     6601 accacaaacc gtgacagcat gacagagcag gccatgccgc agctgtaccg cacactttct
     6661 tccctgcgtt acaccttcag cgaacaggat gatccgcagc gtctgattgg tctgacgcct
     6721 gcggcacaga aaaatatccg tctgctgcgg agcgtcatgc agagtgcaac cctggtgtcg
     6781 caggcgcaga cggtcgggaa actgctggat caggtcatcc ggcagaatac aacgcacgtt
     6841 caggatactg accgtgcggg tggactggcc tggctggaaa gttctgctga tgtgcagcgt
     6901 atcaaccggg atttgagtga tgccatggaa caacaggtac tggatctgtc cgcacagggc
     6961 cataccggca ccgcccttgc attacgtgat gcagggcttg cgctgacaga agatttaacc
     7021 acgcggagtg ttcatcttcc cggcgctttt caggtcatgg ttcgcaccac tgaaccggca
     7081 ctggtcacgc tgtaccgtgc caccggcaac agccgtcgct ggcagtattt tgtccgcaga
     7141 aacaacatcc ccgatccggt ttttgttccg ggtggccgca gcgtggaggt gatcagtgag
     7201 cagcaggatt gaactgtata tcggtggcag tattttttcc ggctggctga cggtcagcgt
     7261 ccgtcgctcg ctggagcatc tggccgggtc gtttgaactg gggctgatgc tgcccggtga
     7321 gcgcatcccc tcagccctgc ggaccggtca gtccctgacg ctcagaatta acgggcagac
     7381 ggtcatcagt ggctggctgg atcaggtcag tcagcggatc agtgcaaccc gtcatcagat
     7441 cagcatcagc ggtcgggata aaaccggcga cctggtggac tgtgccgcca tccatcccgg
     7501 cagccagtgg cgcaaccgga cgctggcaca gattgccgca gatttatgtg cgcccttcgg
     7561 gatagctgtg cgctggcagg ttaacgacga cacggctgca cgccccttca gctcttttac
     7621 gctggaaaac tcagaaaccg tggcggatgc gctgacccgt gcggcacggc atcgcggggt
     7681 gctggtgacc agtaatgctg acggcgatct ggtgttcacc caggcaggaa gccagcagac
     7741 ggacagactg gtgctgggag ataacctgct tgatgccgat tacaacacgg actggcgagg
     7801 acgatacagc gaataccgtg tccggggaca cgggcgcggt ggtggcaaac ggggagacag
     7861 cgaatccgcc gcccggctgg cagcacctgt gggcgttatc agtgatgagc agatcggccg
     7921 ctaccggccg aaaatcatcc tcgccgatca acagacagac gccaccggtg cacggcagcg
     7981 tgccctgcgt gaaatgcgcc gtgcgattgc ccgttcagaa cggttttctg ccaccgtgcg
     8041 tggctggttc cgggatgatg gccggttatg ggatgtcaat ctgctgaccg gtgtttcggc
     8101 cctgcgcttt ggtattgagc agactgaact gctggtctgt caggtggagt ttttactgga
     8161 tgagcagaac ggggaagtca cccggctggt actggcaccg cgtgacggct ttatcgttcc
     8221 ggcagagccg gacagtaagg gccggggtgg ttccggtgac gatgttgatg cctttattcg
     8281 ccagcagatg aaaaaacagg ggatcagctt tgatgaatga tgaagtgttc agccgcctga
     8341 ttgccccggt aacgcgcggt attcgcctgt tgtttggtcg cggcgttctg accggcacac
     8401 atgacgaact gaaaatgcag aatgtgcagc tcaccggcat ggacggcgaa acctttgatg
     8461 atgtggagcg gcccctgcag tacgggcaga tcagcgttcc cctgccgggc gcagaaacct
     8521 tttttgcctg tctgggcggc cagcgggatc agactgtggt gcttgtggtg gaagaccggc
     8581 gcagccgtcc gaccggactc actgccggag atacgggggt gtatcaccat gaggggcacc
     8641 ggatacggtt aacaaaggat ggtcgcgtta ttgtgacatg caaaatgctg gagatttacg
     8701 ccgacgaggg gatgcgggtg gatacgccgg aagccacctt tacgggcaat gtcacggtgg
     8761 ataaaaacct gcatatcaaa ggtaatctga ccattgacgg cacaggaaaa tcacagggta
     8821 cgttcacgat gtccgaggct gttattgccg ggatcaccta ttccggccat gtgcatcagg
     8881 ataacggcaa aggcagtaag acaggagcgc cggagaatgg ctgatattgc aattgtgtgg
     8941 gatcagggat gcggttcgct gcaactcaac ggtgcagacc tgctgacaga taacagcctg
     9001 ctgactgcgg ttctgatttc gctgtttacg gacaggcggg cgctggattc tgatgaaatc
     9061 cctgacggca cccgtgacag gcggggatgg tggggagaca gtttccggga gcgccccgtt
     9121 ggctcccgtc tctggctgtt aagccgtgaa aagacgctgt cctccgtggt cagccgtgca
     9181 caggcctatg ctgatgaagc gctggcgtgg ctgcataaaa gcggcgcagc cacatccgtg
     9241 gtatgtcatg ccatgcgtgt gggacatgat cgcctttcgc tttccgtgaa aatcaccctg
     9301 ccggacggaa gcagacatcc gatgattttt tatgctgata tgaaggggga atgatgcctt
     9361 atcagccctt accactggcg caactgatca cacagacaca acaggatatc agccagcgcc
     9421 tgcctggttc gcagccgggc gtgaatgaaa ccaccctgaa tgccattgcg tatgcacagg
     9481 cggggctgtc agcacaggag catgaacatc tggcctggat ctcccggcag ataattccga
     9541 cagaagctga tgaagccgaa ctcctgaaac actgcgcatt ctggggtgtc atccgtaaac
     9601 cggcttcccg agcagacgga ccggtacaac tgatgctgac cacggatgca gggatcacag
     9661 aaggtgtact ccttcagcga agcgatggtg ttgtgtaccg catcaccggc tctgcgaccg
     9721 gaaaagccgg aacactgaat gttaatgtgg aggcggaaag tgcggggcgc gccggaaatg
     9781 ccccgccagg gacaaaactg acatttatca caccgcaggc ggggatcaac cagacgacca
     9841 cggtgaccgg cacgggactc accggtggtg cggatgtgga aaccgtgccg gagctgcttt
     9901 cccgcctggt tttccgggta cagaatccgc cgtccggtgg cacgcagtac gattttgaac
     9961 gctgggcgcg tgaggtgccg ggcgtgacgc gggcatggtg ccgcccggaa tggccgcagg
    10021 cgggcagcgt gggtgtaacg ttcgttcagg acaacaaccc tgacattttc cccggtgaag
    10081 gtgatgtgaa gcgggtggcg gattatatcc gcagtcatga tgatccggcg acgggccagc
    10141 ccgttggtca gccacttggg ccgacaatca gcgtgtttaa gctgaccaat aagccggtgg
    10201 cgtttgagat caggattgta cccaaaacgc cggaaaatca ggctgccgta aaacaggcat
    10261 taacggacct gctttataac gaatcgcggc cgggtggact ggtgctgcct tcatcattct
    10321 ggcgggctgt tgcaggggtg aaaggactgg aagattttga cgttcgaagt ccgctgaaat
    10381 ccgtgatggc cggagataca gagttgctga ccgtggggga aatcacatgg ctgtaaccct
    10441 gaccccgcat cagcgcgccc tgctgcagtt gctgcctgac ggtctggcat gggacaagcg
    10501 gccgtcatcc gttcttgcgg ctttgtgcct gggtctcagt cattccacgg agcgtgtttc
    10561 ctggatcggt aaccagatgc tggcagaacg ttttcctgat tcatcccgtc tgctgctgga
    10621 agactgggaa cgctatctgg ggttaccgga atgtgatatg accggcgcaa ccattcagga
    10681 acgtcagcgt tatgccggga ataaataccg gatgaaaccc tctcttaacc gtgaatttta
    10741 tatccggttt gcggcagagt ttggttatga aatagatatc cagccatcac cggaatcaca
    10801 atggatcagt attgtgacag tgaagacggc ggtgggatac cgccacatga atgtactgga
    10861 cgatattctt acaccactgc gaatttatga tgcaagtgcg ctggagtgca ttttaaaccg
    10921 ttataagcca gcctggcaga cgtttttata tctgtacgaa aacagtcatg aggagacgga
    10981 gtaatgtatt ttgttgacaa caattcaggt gtgaccgata tgccaccact ggccccctct
    11041 cagggcacgc aggttaaatg gtttactgaa ggtgacggac gcaaagggat cagctatatc
    11101 ggtcaggact ggctgaacat cgtgcaggca gagctgcttg ccattctgac agaaggaaaa
    11161 gttcagcccg ataaggcaaa actgaaccag ctggtgacag ctattaaggc aatcattgcg
    11221 gcaaacgcat acagccggaa aagcaacctt aaggagattg cggatgcagg tgcagaagca
    11281 caggctgccg cccgccgtca tcttgggctt ggaggattat caggaaaaga cagtctggct
    11341 gctaatgatg tgggagcact ggagaaatcc atgaattttg gcgatgtacc ggataaaacc
    11401 tcggcccgcc agaatctgga tgtttacagc aagagcgaag gtgataaccg ttatctgcgc
    11461 aaggatcaga atggcgcgga tatcgcggac aaaggaacct ttatcgataa c
//
LOCUS       DAZGAN020000142        11394 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGAN020000142 DAZGAN020000000
VERSION     DAZGAN020000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.142"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..405
                     /locus_tag="ACHKYO_RS21630"
                     /old_locus_tag="pgaptmp_004326"
     CDS             <1..405
                     /locus_tag="ACHKYO_RS21630"
                     /old_locus_tag="pgaptmp_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HGU4431062.1"
                     /translation="ETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASALIDSY
                     LSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMNGNVPV
                     GVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            402..962
                     /locus_tag="ACHKYO_RS21635"
                     /old_locus_tag="pgaptmp_004327"
     CDS             402..962
                     /locus_tag="ACHKYO_RS21635"
                     /old_locus_tag="pgaptmp_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HGU4431063.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWNDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            963..1208
                     /locus_tag="ACHKYO_RS21640"
                     /old_locus_tag="pgaptmp_004328"
     CDS             963..1208
                     /locus_tag="ACHKYO_RS21640"
                     /old_locus_tag="pgaptmp_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HGU4431064.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            1205..2704
                     /locus_tag="ACHKYO_RS21645"
                     /old_locus_tag="pgaptmp_004329"
     CDS             1205..2704
                     /locus_tag="ACHKYO_RS21645"
                     /old_locus_tag="pgaptmp_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGU4431065.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATEFWGRGSMIALMVAEFIAINPDAELYAIAQGSGT
                     GQAQACLMNIIGTASQDGVLHIYIGGRRYLLAVTKGQKGKELIDALVSVINADADAPF
                     SAYAGQVADNASGLKGSMGINARFTGECSVHDIRVNYHDGETTPGGLTIELGYPKTKA
                     TNPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWMAHTGT
                     FGEVQAFGAKRNDFLATCTFIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALPS
                     RMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLDV
                     ETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWVE
                     LGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            2713..3078
                     /locus_tag="ACHKYO_RS21650"
                     /old_locus_tag="pgaptmp_004330"
     CDS             2713..3078
                     /locus_tag="ACHKYO_RS21650"
                     /old_locus_tag="pgaptmp_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGU4431066.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASATSTRVQ"
     gene            3093..3569
                     /locus_tag="ACHKYO_RS21655"
                     /old_locus_tag="pgaptmp_004331"
     CDS             3093..3569
                     /locus_tag="ACHKYO_RS21655"
                     /old_locus_tag="pgaptmp_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HGU4431067.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            3696..5771
                     /locus_tag="ACHKYO_RS21660"
                     /old_locus_tag="pgaptmp_004332"
     CDS             3696..5771
                     /locus_tag="ACHKYO_RS21660"
                     /old_locus_tag="pgaptmp_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4431068.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGMGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPPSPSSGGAGFWGRMMGRAGGLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGAL
                     WAGAMAAPVLLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNY
                     LGGWLTEAWQKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNV
                     WNGGNYGLW"
     gene            5758..7107
                     /locus_tag="ACHKYO_RS21665"
                     /old_locus_tag="pgaptmp_004333"
     CDS             5758..7107
                     /locus_tag="ACHKYO_RS21665"
                     /old_locus_tag="pgaptmp_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HGU4431069.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGQVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIIDPLFIPGGHSVEVISEQQG"
     gene            7091..8215
                     /locus_tag="ACHKYO_RS21670"
                     /old_locus_tag="pgaptmp_004334"
     CDS             7091..8215
                     /locus_tag="ACHKYO_RS21670"
                     /old_locus_tag="pgaptmp_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HGU4431070.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGAHQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            8205..8819
                     /locus_tag="ACHKYO_RS21675"
                     /old_locus_tag="pgaptmp_004335"
     CDS             8205..8819
                     /locus_tag="ACHKYO_RS21675"
                     /old_locus_tag="pgaptmp_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU4431071.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNITVDKNLHVKGNV
                     SIDGTGRSQGAFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            8812..9249
                     /locus_tag="ACHKYO_RS21680"
                     /old_locus_tag="pgaptmp_004336"
     CDS             8812..9249
                     /locus_tag="ACHKYO_RS21680"
                     /old_locus_tag="pgaptmp_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HGU4431072.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPIGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHDRLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            9249..10331
                     /locus_tag="ACHKYO_RS21685"
                     /old_locus_tag="pgaptmp_004337"
     CDS             9249..10331
                     /locus_tag="ACHKYO_RS21685"
                     /old_locus_tag="pgaptmp_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGU4431073.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            10322..10882
                     /locus_tag="ACHKYO_RS21690"
                     /old_locus_tag="pgaptmp_004338"
     CDS             10322..10882
                     /locus_tag="ACHKYO_RS21690"
                     /old_locus_tag="pgaptmp_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HGU4431074.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEENI"
     gene            10882..>11394
                     /locus_tag="ACHKYO_RS21695"
                     /old_locus_tag="pgaptmp_004339"
     CDS             10882..>11394
                     /locus_tag="ACHKYO_RS21695"
                     /old_locus_tag="pgaptmp_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431075.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGALATKNSLGPGDVNALAKDQNLADLENAGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKG"
BASE COUNT         2492 a         2921 c         3519 g         2462 t
ORIGIN      
        1 gaaaccgata tgcgggcgcg ttaccgcgag gatttgctca ggccgttact ggctgtgccc
       61 cgttcggatg agccggatac gcgcaagctg aaccgggcgc tgacagatgc gtcagccctt
      121 atcgacagct atctgtccgc ccgttacacg ctgccgctgg aggttattcc ggccgttctt
      181 gttcagcact gttgcgcgat tgccttttat tacctgtgcg atcagcgagc ctcagatcag
      241 gcgcgtgacc gttaccgtga ggcgctggcc tggctgaaag atgtcatgaa cggcaatgtg
      301 ccggtaggtg tggatacgaa cggagcggcc cctgaatccg gggatttacc acaggttcag
      361 tctgatgcag cggtattcgg gcgcaaccag aagggcttca tatgattacg gaaaccgaac
      421 aggcatacat cgcccgtatc cgtgagtatt tcgggaatga actggtgtct gttgacacgc
      481 atcccggcga ctggaacgac agcgtactgc gcaccatgct gattaacgcc ccggcgatct
      541 acgttgcctg gctgggtgcc ggtgaaggcc gtacccgtgg tcgcctggtc agtcactggg
      601 tgttctacgt catcggcgac atgctcaacg ggcgtgaggc cagccgtccc ggattgtatc
      661 agattgtggc ccggctgatt gccgtgctta acggcttcag aacagaaaaa acctcaccgc
      721 tttactttga aaaggcggtc aacggttaca ccgaaaccca ggcggacagt ggtgcggtga
      781 tgtatgcgct ttatttctcc tgcgaggaaa tgatcgcacc gctgaccgat atcagctcgc
      841 tggacgactt cctgcgtcat tacgaaacct ttgtggagcc gcccggtacg ccgccgtttg
      901 aggcacatat tgatttgccg gggaacacga caacggcggt cgaaccgccg gaggaactgt
      961 aaatgaaaac catctttatc aaacccgcgc cgggacgcct gattcgtgat cccgacacaa
     1021 tgcgcccgct ggcacaggag ggggaggaaa agccctttac gccgttctgg tgtcggcgtc
     1081 ttgatgacgg cgatgtcatt caggctgaaa aagccgctga agaggctccg gctgtatctg
     1141 cggatgcaac gactgcgaca gaaaaaccgg tcgcccaacc ggcatccgat aaggagaaac
     1201 cgcaatgatt aattttgacg ggatcggtaa tgaccgccgt ataccgctga ttgaggttga
     1261 gtttaataac tccatggctg tcaccggcac tccggcacag cgtcagtgtg tgctgctgtt
     1321 tggtcaggcg agaatgaaag agaatgccgt ggacggtgcc ggtgttctgg atgttccggt
     1381 caggatcacc cgtgcgtctc aggcaacaga attctggggg cgcggctcca tgattgccct
     1441 gatggtggcg gaatttatcg ccatcaatcc tgatgctgaa ctgtatgcca ttgctcaggg
     1501 gagcggcacc ggtcaggctc aggcatgtct catgaatata attggcaccg cctctcagga
     1561 tggtgttctg cacatttata tcggcggtcg gcgttatctg ctggctgtca cgaaggggca
     1621 gaaaggtaag gagcttattg acgctctggt ctctgttatt aatgccgatg ctgatgcgcc
     1681 cttcagtgct tatgcaggtc aggtagcaga taacgccagc ggactcaagg gaagtatggg
     1741 aattaatgcc cgttttaccg gtgaatgttc cgtacatgat atccgtgtta attatcatga
     1801 cggagaaacc acaccgggtg gactgaccat tgaacttggg tatcccaaaa caaaagccac
     1861 taacccggat atcacccgaa gcgtggcggg tatgggcgat cgccagtaca actatgtcgt
     1921 catgccctat aaggatgatg caaacctgaa aatcatcagt gatgagcttc tgaaacgctg
     1981 gggaccggcc aaaatgtctg acggtgtgct gtggatggcg cataccggca cgtttggtga
     2041 ggtgcaggca ttcggggcaa aacgcaatga ttttctggcg acctgcacat tcattcccaa
     2101 agcccctgaa ccggattacc tctgggcagc ggcggtctgc gccacctgtg cgccgtctct
     2161 gggggcagat cctgcgcgtc cgcttcagac gctggcgctt ccgtcacgca tggccccggc
     2221 cccggccgat cgtctgacgc gtgaagagcg taacagtctg ctgtggggag ggattgccac
     2281 ggtcagtgtg gctgccggtg atgtggttca gatcgaacgt caggtgacca tgtatcgcca
     2341 gaacgtgtac ggcgaaagtg atcccagcta tctggatgtg gaaaccatct acaccctctc
     2401 ctggctgcgt tattccctgc gtacttttat cacacaacgt tttccgcgcc ataaactggc
     2461 tgatgacggg acgcccgttc gtgccggtca gaatatcgtg acgccggaga tcatgaagct
     2521 tcagttaatt tcgctgggtg aagagtgggt ggaactcggg ctggtcgaaa atctggacac
     2581 ctttaaaaag aatctgcttg tggagcgtaa tgcgtcagat cgaaaccgtc ttgacgtgtt
     2641 atgcacgccg gatctggtta accagttccg cttcctggca gcacagatcc gtttcatttt
     2701 gtgaggtaag gcatgagcgg aaaacagtat cagggcacgg ccactatccg tgtgaacggt
     2761 caggaatacg agacgcttga gggggctaca ttttctccgt caggttttga acgtgaagtg
     2821 gtgaaaggcg cgaaagtcta tggttaccgc cagaaaccac gtgaagcgac actggactgc
     2881 aaatttccgg caggcggaga aggttcacca gccgccgatg aaatcaacaa ctggactgct
     2941 gtcactatcg agtttgtggc ggataccggc gaagtccaca tgatgacgaa ggcctggagc
     3001 agtgaaccgg cctcgcttga cggtggcggg gagatctccg tgaagttcgc cagcgccacc
     3061 agtacccgtg ttcagtgatc aggaaaaata aaatgaccac acgtaagaaa aaaacggcag
     3121 tttccgaggc agccgtgatg gaggcaatcc gggaagcact tgagggggct gatccacgca
     3181 ctgccgggct gacagagcaa ctggcaaagg gatatgtgga tctgcttgat ggtctgccgt
     3241 ttggtgagac ccgtgaatat cgtgtcacgt tccgggaact gacagcgaaa gacagtattg
     3301 acgcagaagc agaagccgag cgcgtggtgg agacaaacaa tggcccgatg ctgatagcgt
     3361 ctccgtcgtt acgcggtgtt gcactgctgc gccgtcagat tgccgcagtg ggagacattg
     3421 aaggtccgtt gtcaccccgt cagattggac agttaagcga gcgcgatctc tcccgcctga
     3481 tggcagcggt cagcctgctg gataccgcgc ttgccggaaa gctggcagct gaccgggggc
     3541 gatcaggcgc agtgtcggga tcagattgaa gaagcggcaa ttatcctggg gatggtgaca
     3601 aaaagtggcc cggaatgggc gcttaacctc cccctgtcgc aactttaccg gcactgccga
     3661 cagacagaaa aaatcatcag aacgaagcag taagtatggc ccgaaatctc agagcatctc
     3721 tgatagttga tttgctcggc aatatctcag ccaaatcccg ccagtggtct caggagctgg
     3781 gggcattctc acgctccggc caggcggggc tgggtggtct gggaaatgcg gcccgccgtg
     3841 ccgggcagga aacagatgta ctcggaagca ggatgcagcg cacgctggcc ggggtgcggg
     3901 gcagtatccg ccatgtcact tctgattttg accgtcttca gggcagtatt accggcacca
     3961 tcgggcggat cagcaatctt tacggaatgc tggccggtgg tgcagctgta tatggcttta
     4021 accggggatt tattcgcccg gcagcggaga tggaaaccta tatgattcgc ctgaactccc
     4081 tttacaaagg ggatcgggcg aaaactgatg atgtccgcag gtgggcaata cagaatgcga
     4141 aggaaaccac ctgggggctg gctggcgtca tgcaggaata tacgtccagt ctgggctttg
     4201 gtatgagtga cagggaggcc cgaaattttg tcaccatgct tcaggatcag ggtgccgtgg
     4261 gcggctggtc actgtccgat gcacaggggg catccctgca actgaaacag atgtatgcaa
     4321 gaggcagtat tcaggcagcg gatgcaaata ttcttgccac ctacggcatt aatgcctacc
     4381 gcgtgctggc tgatcgtctg ggtgtggacc agaaagtggt cagaaaactt ggtgaaaaag
     4441 ggctgcttgg gccggacagt attcgcctgt tatttcagac gctggcggaa caggccaggg
     4501 gagcacaaaa ggacgcaatg aattcctgga gtggcctgac ggccatgatg ggtgacgtct
     4561 gggatcagtt tgcccgggat gtgatggaca gtgggccatt tgaaaaactg aagggtaatc
     4621 tcaggggatt tctttcatgg gttgacagtg ccaaatctga tggcagttac cattccctgg
     4681 cggaaagcac ggcatcagcc atgaaccagg gctttgagta tgcccgtgat gcagtgacag
     4741 ggttttatca ggcgatcaga aaggtacgcg acacgcttca ggctctgcgg gatgcgggct
     4801 atggtgatgc gctggatcgc attggtcagg gggcacagac cgccgcgaaa tacctgctgt
     4861 acatgtatct cgccacccgt gccctgaaaa tggcgcgggc tgtcggaatg gggattatac
     4921 gtccgggtgc tgcattgctg ggatacggat tatcagcggc ggccttcctg acttcacctt
     4981 tccggcgctc tcctccggcc ggaacaccac caggcacatc tcagggacga gggcaacgtt
     5041 ttataaactt tcttaccggg gtaaatccgg cagccgttca gccagtactt gtaacaaact
     5101 ggcccacagg aggactgtcc ggcaccggcg cgaccgatac ttcctcaggc ggacgtcagg
     5161 ggcggggccg caggaaacgc ggaccggggc gcggaagacc ggtcacgcct cccctgccac
     5221 cgtccccagt gccaccgtct ccctcttctg gtggggcagg gttctggggg cgcatgatgg
     5281 ggcgcgccgg tgggttactt tcgtctgtcg gtagccgcat ggggctgggg cgtttctccg
     5341 gcttttttcg cagtgccggt ggactggccg gccggctggg tggcggtgcc ctgtgggctg
     5401 gcgcgatggc agccccggtt ctgctggatg gcagcgccag tgccacagac aagggagaag
     5461 cggtcggctc tcttgcaggc agtattgccg gtggtgcgct gggggcggca gccggacctg
     5521 tggggattgc cattggttct acggtgggca gctatctcgg taactatctg gggggatggc
     5581 tgactgaagc ctggcagaaa ttacggggca gtgatgatga aagcagtgga caggcggtac
     5641 aaaaagcctc tgcccgtgtg gagctggttg ccccggaagg gtggcaggct cgcagtattg
     5701 atattgatga cacgtccggt cacggactgg atgtgaatgt ctggaacgga gggaattatg
     5761 gcctctggtg acggtcgtgg tgcgttccgt ggtgtgccgt tcctcgtctg gcgtgagcag
     5821 cgcgaacgtg gcgggcgaaa cattgtccgt cgggaatatc cgttacggga aaccggtggg
     5881 gcagacgatc tgggacctaa actggctgaa tttacgttca gtgtacttgt aatgggggat
     5941 gatgttcaga cgcagcgaaa tcgtctgcgt gatgcccttc gtgctcccgg tgccggggag
     6001 ctgcttcacc cggattacgg cacgttaaat gtgctgataa acagctttga aagtcgttat
     6061 aacgccgctg aacagggcgt ggttgagttc accattaatg tcacgccggt aagtgatgat
     6121 accgcacctg ctgtcacaca ggacacggcc gccattctgg agcagaagag taccaccgcg
     6181 ctcgggcagg tttttgaaac cctggaagcg ggctggacgg tgatctctga cggaatgcac
     6241 gatgtccagg caatgaccga aaccatcagt gataaggtgt ccgccctgga aaatgccgtt
     6301 tccggcatgg ggattgtaca ggatatcagt gcctttactg ccacgttcac ggcgctgaaa
     6361 gggaatgcca cggcattact gacagcacct tcccgcatgg cctcatcttt tgcggggctt
     6421 ttcagtgccc tgatcaccct gccatcgctg ccgtcactgt cttcaggagg cctgaatacc
     6481 cggcccggtg gcagtatcgg ccggacgaca tccgccgtct ctcagggaat gccacaactg
     6541 tacaggacat tatcttccct gcgttacgtg ctggatgagc aggataatcc gcagactctc
     6601 atcggtctga caccggcagc acaaaagaat attcgtctga tacgggcggt aatgcagagt
     6661 gctgccgtgg tggcccaggc acagaccgtg ggaaaactgc tggatcaggt tctcagtcag
     6721 gaaaccctgc cggacagtga cgcagcccac cgtacctggc ctgtctggct ggaaagttca
     6781 gttgatcttc agcgcattaa ccgtgactta agcgaagcgc tcgaacggca ggtgatgaca
     6841 ctgtccgggc aggggtatac cgccacggcg ctgacgcttc gtgatgccag actggcactg
     6901 acagaggatc tgaatacacg gggagttcag ctacccggtg caacagtagt gactgtacgt
     6961 accaccgagc ctgcactggt gaccctgtac cgtgccaccg ggaacagtac cggctggcaa
     7021 cgttttgtgc gccgtaacgg tattattgat ccgctgttca ttcccggagg ccattcagtg
     7081 gaggtgatta gtgagcagca gggttgaact gtatctgggc ggtgagattt tttccggctg
     7141 gctgacggtg agtgttcgtc gctctcttga acatctggcg ggctcctttg aactgggggt
     7201 aatgatgccc ggtgtacgcc ttccgtcatc cgtccgtgcc ggtcagtctc tggaattgcg
     7261 cattgacggt cagcctgtga tcactggctg gctggatcag gtccggcagc gcatcagcgc
     7321 cacgcgtttt cagatcacgc tcagcggacg ggataaaacc ggtgacctgg tggactgttc
     7381 agccattcat ccgggcagcc agtggaggaa ccgcacgctg gagcacattg cttcagattt
     7441 gtgtgctccg ttcggggtca cggtgcgctg gcaggtaaat gatgcaacgg cagcccggcc
     7501 cttttccacc ttcacactgg aaaattcaga aaccgtggca gatgcgctga cacgggccgc
     7561 gagacacaga ggcgtcctgg taaccagtaa tgccgccggt gaactggttt tcactcaggc
     7621 cggcagtcag cgtggtgaca cgctgacgct gggcgaaaat ctgctggatt tggatcacaa
     7681 cgtggatcac cgtctgcgcc acagtgaata ccgtgtacgg gggcacgggc gtggtggtgg
     7741 tcatgccggg gatgcactga cagccggaac gctggccgca ccagttggta cggtgacaga
     7801 cagtgccatc caccgttaca gaccgaaaat tgtgctggcg gatcatgctg ttgatgcaga
     7861 cggtgcacac cagagggctg tccgggaaat gcgccgggcg gttgcccgct ctgtgcgcct
     7921 gacagccacc gtgcggcact ggtttcggga gaacggccag ttgtgggata tcaacctgct
     7981 gacggctgtc acggctcccc gcaccggagt ggaagagcgt gatcttcttg tctgtcaggt
     8041 ggagttttcg ctggatgcaa atcacggcga aaccacccgt ctgattctgg caccccgtga
     8101 cggctttatt gttccggcag agccgggaaa cagcggaagc ggaaatgcgg gtgacgtgga
     8161 cgccttcgtg cgggcacaga tgaaaaaaca ggggattaaa ttcaatgatg aatgacgaag
     8221 tcatcagccg ccttctggcc cccgtgatgc ggggtgttcg tctgctgttc gggcgtggtg
     8281 tactgaccgg cacaacggac acgctgaaaa tccagaatgt gcaaatcacc ggtatggatg
     8341 gtgaaacctt tgatgacgtt gaacgccccc agcagtacgg gcaaatcagc gtccccctgc
     8401 ctggtgcgga agtttttctg gcctgtgctg gcggacaacg ggatcaggcc gtggtgcttg
     8461 tggtggaaga ccgccgcagt cgcccgaccg gacttaccgc cggagatacc ggcgtgtatc
     8521 accatgaggg gcaccgcata cgtctgacaa agaacggccg gatcattgtg acctgtaaga
     8581 cgctggagat ttacgccgat gaaggtgttc aggtggatac accggaggct cactttaccg
     8641 gtaatatcac agtggataag aacctgcatg tcaaaggcaa tgtgtccatt gacggcaccg
     8701 gaagatcaca gggggcgttc acgatgtccg aagcggttat tgccgggatc acctattcag
     8761 gtcatgtgca tcacgataac ggtgaaggca gcaaaacggg aggaccagag aatggctgat
     8821 attgctgtcg tctgggatca gggttgtggt tccctgcaac tgaacggcgc agatcttctg
     8881 acggataaca gcctgctgac tgcggtcatt atttcactgt ttacggacag gcgggcgctg
     8941 gattccgatg aaatccctga cggcacccgt gaccgtcggg gatggtgggg agacagtttc
     9001 cgggagcgcc ccattggctc ccgtctctgg ctgttaagcc gtgaaaagac gctgtcctcc
     9061 gtggtcagcc gtgcacaggc ctatgctgat gaagcgctgg cgtggctgca taaaagcggt
     9121 gctgccacat ccgtggtatg tcatgccatg cgtgtggggc atgatcgcct ttcgctttcc
     9181 gtgaaaatca ccctgccgga cggaagcaga catccgatga ttttttatgc tgatatgaag
     9241 ggggaatgat gccttatcag cccttaccac tggcgcaact gatcacacag acacaacagg
     9301 atatcagcca gcgcctgccc ggttcgcagc cgggcgtgaa tgaaaccacc ctgaatgcca
     9361 ttgcgtatgc actggcgggt ctgtcagcac aggagcatga acatctggcc tggatctccc
     9421 ggcagataat tccgacagaa gctgatgaag ccgaactcct gaaacactgc gcattctggg
     9481 gtgtcatccg taaaccggct tcccgcgctg acggaccggt acaactgatg ctgaccacgg
     9541 atgcagggat cacggaaggc gtactccttc agcgaagcga tggtgttgtg taccgcatca
     9601 ccggctctgc gaccggaaaa gccggaacac tgaatgttaa tgtggaggcg gaaagtgcgg
     9661 ggcgcgccgg aaataccccg accggaaccc gcctgtcctt tatcacgcca caggcgggca
     9721 tcaaccagac agccacggtt accggcacgg gactcaccgg tggtgcggat gtggaaacgg
     9781 tgccggagtt gctgtccagg ctggtattcc gggtacagaa cccgccatca gggggaacac
     9841 agtatgattt tgaacgctgg gcacgggaag taccgggcgt gacgcgggca tggtgtaagc
     9901 ctgaatggcc tgaggcgggt agtgttggtg tgacttttgt tcaggataat aaccctgaca
     9961 ttttccccgg agaaggtgat gtgaagcggg tggcggatta tatccgcagt catgatgatc
    10021 cggcgacggg ccagcccgtt ggtcagccac ttgggccgac aatcagcgtg tttaagctga
    10081 ccaataagcc ggtggcgttt gagatcagga ttgtacccaa aacgccggaa aatcaggctg
    10141 ccgtaaaaca ggcattaacg gacctgcttt ataacgaatc gcggccgggt ggacttgtat
    10201 tgccttcatc attctggcgg gctgttgcag gggtgaaagg actggaggat tttgaagttc
    10261 gcagtccgct gaagtccgtg atggccggag atacagagtt gctgaccgtg ggggaaatca
    10321 catggctgta accctgaccc cgcatcagcg cgccctgttg cagttgctgc ctgacgggct
    10381 ggcatgggat aagcggccgt catccgttct tgcagctttg tgcctgggcc tcagtcattc
    10441 cacggagcgt gtttcctgga ccggtaacca gatgctggca gaacgttttc ctgattcatc
    10501 ccgtctgctg ctggaagact gggagcgtta tctggggtta ccggaatgtg atatgaccgg
    10561 cgcaaccatt caggaacgtc agcgttatgc agggaataaa taccggatga aaccctctct
    10621 taaccgtgaa ttttatatcc ggtttgcggc agagtttggt tatgaaatag atatccagcc
    10681 atcaccggat tcacagtggg tcagtattgt cacgataaac agtgaaaccg gctaccggaa
    10741 tatgaatgtg ctggatgata ttctcacgcc gctgcgtatt tatgagggcg gtgcgctgga
    10801 atgtattctg aaccgttata agcctgcatg gcagacgttt atttacgtat atgcaaacag
    10861 tcacgaagag gagaatattt aatgttccat gttgataata attccggcgt ggcgaatatg
    10921 cctgcgctgg caccggcgca gagtaatacc accacctggt ttaccgaagg tgacggacaa
    10981 aaaggtatca gctggattgg tcaggactgg ctgaatattc tccaggccga actgctgaat
    11041 attctggctg aagccagtat tcagccggat aaggcgcagt taaaccagct tacgctgtcc
    11101 attaaagcca ttatcgctgc gaatgccttt tcccggaaaa acaacctgaa agaaattgct
    11161 gatgccggtg cggaagccca gcgtcttgcc cgtggttatc ttggtcttgg ggcgcttgcc
    11221 acaaaaaaca gtcttggtcc cggtgacgtt aatgctctgg cgaaggatca gaaccttgcc
    11281 gacctggaga atgcgggaac cgcccgtaat aatctggatg tttacagcaa aagcgaaggt
    11341 gataaccgtt acctgcgcag ggagcagaac ggcgcagaca ttccggataa aggc
//
LOCUS       DAZGAN020000143        11375 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGAN020000143 DAZGAN020000000
VERSION     DAZGAN020000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.143"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..393
                     /locus_tag="ACHKYO_RS21700"
                     /old_locus_tag="pgaptmp_004340"
     CDS             <1..393
                     /locus_tag="ACHKYO_RS21700"
                     /old_locus_tag="pgaptmp_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HGU4431076.1"
                     /translation="ERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQRRELVA
                     LRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADC
                     HVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            408..1265
                     /gene="menB"
                     /locus_tag="ACHKYO_RS21705"
                     /old_locus_tag="pgaptmp_004341"
     CDS             408..1265
                     /gene="menB"
                     /locus_tag="ACHKYO_RS21705"
                     /old_locus_tag="pgaptmp_004341"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HGU4431077.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1265..2227
                     /gene="menC"
                     /locus_tag="ACHKYO_RS21710"
                     /old_locus_tag="pgaptmp_004342"
     CDS             1265..2227
                     /gene="menC"
                     /locus_tag="ACHKYO_RS21710"
                     /old_locus_tag="pgaptmp_004342"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HGU4431078.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            2224..3579
                     /gene="menE"
                     /locus_tag="ACHKYO_RS21715"
                     /old_locus_tag="pgaptmp_004343"
     CDS             2224..3579
                     /gene="menE"
                     /locus_tag="ACHKYO_RS21715"
                     /old_locus_tag="pgaptmp_004343"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HGU4431079.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3689..3955
                     /gene="pmrD"
                     /locus_tag="ACHKYO_RS21720"
                     /old_locus_tag="pgaptmp_004344"
     CDS             3689..3955
                     /gene="pmrD"
                     /locus_tag="ACHKYO_RS21720"
                     /old_locus_tag="pgaptmp_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HGU4431080.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(3949..4335)
                     /gene="arnF"
                     /locus_tag="ACHKYO_RS21725"
                     /old_locus_tag="pgaptmp_004345"
     CDS             complement(3949..4335)
                     /gene="arnF"
                     /locus_tag="ACHKYO_RS21725"
                     /old_locus_tag="pgaptmp_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HGU4431081.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4335..4670)
                     /gene="arnE"
                     /locus_tag="ACHKYO_RS21730"
                     /old_locus_tag="pgaptmp_004346"
     CDS             complement(4335..4670)
                     /gene="arnE"
                     /locus_tag="ACHKYO_RS21730"
                     /old_locus_tag="pgaptmp_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HGU4431082.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4667..6319)
                     /gene="arnT"
                     /locus_tag="ACHKYO_RS21735"
                     /old_locus_tag="pgaptmp_004347"
     CDS             complement(4667..6319)
                     /gene="arnT"
                     /locus_tag="ACHKYO_RS21735"
                     /old_locus_tag="pgaptmp_004347"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HGU4431083.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6319..7209)
                     /gene="arnD"
                     /locus_tag="ACHKYO_RS21740"
                     /old_locus_tag="pgaptmp_004348"
     CDS             complement(6319..7209)
                     /gene="arnD"
                     /locus_tag="ACHKYO_RS21740"
                     /old_locus_tag="pgaptmp_004348"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HGU4431084.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7206..9188)
                     /gene="arnA"
                     /locus_tag="ACHKYO_RS21745"
                     /old_locus_tag="pgaptmp_004349"
     CDS             complement(7206..9188)
                     /gene="arnA"
                     /locus_tag="ACHKYO_RS21745"
                     /old_locus_tag="pgaptmp_004349"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HGU4431085.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9188..10156)
                     /gene="arnC"
                     /locus_tag="ACHKYO_RS21750"
                     /old_locus_tag="pgaptmp_004350"
     CDS             complement(9188..10156)
                     /gene="arnC"
                     /locus_tag="ACHKYO_RS21750"
                     /old_locus_tag="pgaptmp_004350"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HGU4431086.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10160..11299)
                     /gene="arnB"
                     /locus_tag="ACHKYO_RS21755"
                     /old_locus_tag="pgaptmp_004351"
     CDS             complement(10160..11299)
                     /gene="arnB"
                     /locus_tag="ACHKYO_RS21755"
                     /old_locus_tag="pgaptmp_004351"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HGU4431087.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
BASE COUNT         2768 a         2955 c         3046 g         2606 t
ORIGIN      
        1 gaacgtcagc gttccgatcg ccaatgggcg cagcgttttc gcacagaacc gttaacggcg
       61 gtatttgccg actggtatca acagcctgtt tttgcctcgc tcaatgacga tcaacgccgg
      121 gagctggtgg cgctgcgcag caacaataat ggcgcaaccc ttgccgccat gctggaggcg
      181 acttctctcg ccgtccagcc tgatttacgt gctaacctta gcgcccgcac atttgcgttt
      241 tattatttat gtggtgaacg tgacagcaaa ttccgcgccc tggcggcgga actggctgcc
      301 gactgccatg tcattcctcg cgccggacat aacgcgcatc gggaaaatcc cgctggcgtt
      361 atcgcaagtc tggcgcagat cttgcgtttc tgactaaagg acacaatatg atttatcctg
      421 atgaagcaat gctttacgca ccggttgaat ggcacgactg ctccgaaggt ttcgaggaca
      481 ttcgttatga aaaatccacc gacggtatcg caaaaatcac cattaatcgt ccgcaggtgc
      541 gcaatgcctt ccgtcctctg acggtaaaag agatgatcca ggcactggca gatgcgcgtt
      601 atgacgacaa tatcggcgtg atcattctga ctggcgcagg cgataaagcg ttctgctccg
      661 gtggtgacca gaaagtgcgt ggtgattacg gcggctataa agatgattcc ggcgtacatc
      721 acttgaacgt gctggacttc cagcgtcaga tccgtacctg tccgaaaccg gttgtcgcga
      781 tggtggctgg ctactccatc ggcggtggtc acgttctgca catgatgtgc gacctgacta
      841 tcgcagcaga taatgccatc ttcggtcaga ctggcccgaa agtcggttcc ttcgacggcg
      901 gctggggcgc ttcctacatg gctcgtatcg tcgggcagaa aaaagcgcga gaaatctggt
      961 tcctgtgccg tcagtacgac gcaaaacagg cgctggatat gggccttgtc aacaccgtgg
     1021 taccgctggc ggatctggaa aaagaaaccg tccgttggtg ccgcgaaatg ctgcaaaaca
     1081 gcccgatggc gctgcgctgc ctgaaagctg cgctgaacgc cgactgtgac gggcaggcgg
     1141 ggctgcagga gctggcgggc aacgccacca tgctgttcta catgacggaa gaaggtcagg
     1201 aaggtcgcaa cgctttcaac cagaaacgtc agcctgattt cagcaaattt aaacggaatc
     1261 cgtaatgcgt agcgcgcagg tataccgctg gcagatcccc atggacgcgg gggtggttct
     1321 gcgcgacagg cggttaaaaa cccgcgacgg gctgtacgtt tgcctgcgtg agggcgagcg
     1381 cgaagggtgg ggggagatct ccccactgcc gggcttcagc caggaaacct gggaagaggc
     1441 gcaaagcgtg ctgcttgcct gggtaaataa ctggctggca ggcgattgcg agctaccgca
     1501 gatgccttcc gttgcctttg gcgtaagctg tgcattggcg gagctggcag atacgctccc
     1561 gcaggcggcc aattaccgtg cggcaccact gtgtaatggc gatcctgacg atctgatcct
     1621 caaacttgct gatatgccag gcgagaaagt ggcgaaggtc aaagtgggat tgtacgaagc
     1681 cgtgcgcgac ggcatggtgg ttaatctgtt gctggaggca attccggatc tgcatttgcg
     1741 tcttgacgca aatcgcgcct ggacaccgct gaaaggccag cagtttgcca aatatgttaa
     1801 cccggattat cgccaccgca tcgcgtttct cgaagagccg tgcaaaaccc gcgatgattc
     1861 gcgagcgttt gcccgtgaaa ccggcattgc cattgcctgg gatgaaagcc tgcgcgagcc
     1921 ggattttgcc tttgtggctg aagagggcgt gcgcgcggta gttatcaaac ccacgctcac
     1981 gggcagtctg gagaaagtac gcgagcaggt acaggcggcg cacgcgctgg ggctgacggc
     2041 ggtgatcagt tcttccattg aatcgagctt aggcttaacg caactggcgc ggattgccgc
     2101 ctggttaacg ccggacacca ttccagggct ggacacgctg gatctgatgc aggcgcagca
     2161 ggtacgtcgc tggccgggta gcacgctgcc tgtcgtggaa gttgatgcac tggagcggtt
     2221 gttatgatct tctctgactg gccgtggcgt cactggcggc aagtgcgggg agaagccatc
     2281 gccttacgtc ttaatgacga gcaacttaac tggcgcgagc tttgtgctcg cgtcgatgaa
     2341 ttagcctcag gctttgcggc gcagggcgtt gttgagggca gcggcgtgat gttgcgggcg
     2401 tggaatacgc cgcaaacgct gctcgcctgg ctggcgttac tgcaatgcgg ggcgcgggtg
     2461 ttgcccgtga accctcagct gccgcaaccg ttgcttgaag aattgctgcc caatctgacg
     2521 ctgcaatttg cgctggtgcc ggatggggaa aacacgtttc cggcgttaac gtcgctgcac
     2581 attcagcggg ttgaaggcgc acatgccgct acgtggcagc cgacgcgtct gtgctcaatg
     2641 acgttgacct cgggttctac cgggttgccg aaagccgctg tccatactta tcaggcccat
     2701 cttgccagtg ccgaaggggt gttatcgttg attccgtttg gcgatcacga tgactggttg
     2761 ctctctttac cgctgtttca cgtctccggt cagggaatta tgtggcgctg gttatacgct
     2821 ggtgcgcgga tgacggtgcg tgataaacag ccattggagc aaatgctggc aggctgtact
     2881 cacgcttcac tggtgccaac acaactctgg cgtttgctgg ttaaccgcag ttccgtttcc
     2941 ctgaaagcgg tgttacttgg cggcgcggct atcccggtcg agttgacgga acaggcgcgc
     3001 gagcagggga ttcgttgctt ttgcggctat ggtctgaccg agtttgcctc cacggtgtgt
     3061 gcgaaagaag ccgacggcct ggcagacgtt ggttcgccgc tgccgggtcg ggaagtgaaa
     3121 atcgttaata atgaagtgtg gctgcgggct gccagtatgg cagaaggtta ctggcgtaac
     3181 gggcaactgg tttcactggt taatgacgaa ggctggtacg ctacgcgcga tcgcggtgag
     3241 atgcataatg gcaagctgac cattgtcgga cgtttagaca atctgttctt cagtggcggt
     3301 gagggtattc agccggaaga agtcgagcgt gttattgctg cacatcctgc ggttttgcag
     3361 gtgtttatcg ttcccgttgc cgacaaggag tttggtcatc ggccggtagc ggtggtggag
     3421 tatgaccagc aaaccgttga tcttggcgaa tgggtgaaag ataagctggc tcgttttcaa
     3481 caaccggtgc gctggctgac tttaccgccg gagctgaaaa acggcggtat taaaatttca
     3541 cgtcaggcgc taaaagagtg ggtgcaacgt cagcaataat taaccctccg tttcacttct
     3601 catttctgcc attccattgc cgttgataaa gagtttatct gttaatctgt aaattgatgt
     3661 gaaaactctt agcaaacagg ataatgcaat ggaatggctg gtcaaaaaat cgtgctgcaa
     3721 taaacaagat aacagacatg tacttatgct ctgcgatgct ggcggcgcga taaaaatgat
     3781 cgccgaagtg aagagtgact tcgccgtgaa agtgggggat ttactctcgc ctttgcagaa
     3841 tgcgctttat tgtattaatc gtgaaaagct gcatacggtg aaagtcctct cggcaagcag
     3901 ttatagcccc gatgagtggg aacggcagtg tacagcggca gggaaaactc agtaacgttg
     3961 ttttgtcgtg ggcaggaaaa tcagcatcaa cccgctcata atacaggcta ctcccagtag
     4021 tgctttcagc gaaaaagtcc cttcccagcc gggaagtacc attgaagcaa tccataccag
     4081 cacataactc atactgagca aggcataggc tttgcttagc gcaagtttat gtagcgtttt
     4141 ataccaacaa aaaacggaaa gcagataacc cagcagcccg agtaacagta tccgggcatc
     4201 caggccaaaa gctaacagag ccgcgataaa atcccataag tgcgtcatcg gcggcagatg
     4261 acttgccgca aaacccaggc ttaattgcgc aactgaggca ataatgacgc tgaataatcc
     4321 ccatatcagg cccattacac cgtactcccg aggatcacaa tgccaccaat aatgaacgcc
     4381 accccacacc agtgacgcgg cgataccggt tcgtgccaca gttttactgc agccagcgtc
     4441 acccagacaa aattcagact taacatcggg taggcaatgc ctaccggtac gttctgcaag
     4501 accagcagcc agagcaccat ggcaagacca agacaagcca gcgccagtcc cagccacagc
     4561 acgatatgtt tgcgccgttt gtttatcgcc acaaagcagg ttgcctgttt ctgacacaac
     4621 tgcccggcaa cgctaagcaa gctggcaaag actaatgtta gccagatcat ttgggacgat
     4681 actgaatcag caccagacgc tcctgacgat cgatggcatc tgcgggcgga atggcgagac
     4741 tgttgatatc ttcatcgcgg tcaaccgaaa gcacgagagt aataatcccc tcctgacgat
     4801 gttgattaag ccagtttgcg aactcatcac cgctgacaaa tctccctttc gcatccggat
     4861 aattaaggcc gtatttcaac tcacctgtct ggcgatacat gatgatgtca tcgcgttgca
     4921 ggctccatgc cagacctgcg gcaacaccga cgctatcagt aagaatatag cggcttggct
     4981 gaagtgattc ttgtgtcatc tcgacaaaaa attgcggatg ttttccttcc ataactctgt
     5041 caggaattga aaatcctacc agcaacgcca gccccagcgg gcaaagcgcg gcaaaaggcc
     5101 aggtcttttc gacgtttgtt aaggtgtacc agccgaaaaa tgcccatagc gaaaaaatcg
     5161 accaggcaca aaagacttta tagctctcga aggtttgcca caccggcgtg ttcattggtc
     5221 cccacgggga gaccacaaat gtggcaataa tgccagtgac gccaaaagcg atgttaatcc
     5281 agccattaat ccgcagcgcc agaggattat tttttgctgc cagcaaagcg taatgcgcca
     5341 tcagcattgc cagagatgca aagcaggaaa gaatataggt gggcaattta cctttagcga
     5401 cggagaaaaa cagcagcggc attatcgtcc agctcaacaa atagacggtt gcggaatgct
     5461 tgcggttttt ccagcctgtg tacagtgcac cggggagtaa tcccagccac ggcaggctac
     5521 cggcaatgat gaccggcacg tagtaccaga acggagctct atgttgggca tcatccagtg
     5581 caaagcgttg aatatgctca acccagaaaa aatagtgcca gaagtcaggc tcccgctgcg
     5641 ctatcgccag tccccaaggg agaaccgtca gtacgcaact gataaccgcc agccagccgt
     5701 aaataaagag atctttccag cgtttttgcg tcgctaccca tggcagcacg cttaataccg
     5761 gcacggcaag ggcgagaaaa cctttggtca tcacccccat gccgcaggtg attcccagca
     5821 gtaaaaatcc tgcgcttttg cctttccacg tctgtgcctg cattgccagc cagaagctgc
     5881 acattcccgc caccagccag aatgcaataa acggatcgag cacggcataa gtgccgatgg
     5941 catagacaat aaacaatgag agataaatta ctgtggcgag tagagccaga cgtttatcgc
     6001 gccataagcg cagagtaaac caggtcacca gcgcggcagt taacagggtc gcaaagataa
     6061 cgcctgcccg cacaccaaag ttattcgcgc caaatagcca ttgcccaatg ctgttaatcc
     6121 agtatccggc aatgggtttt tcgaaataac gtagccctaa cagatggggc acaatccagt
     6181 cgccggatgc cagcatttct cgactgattt ccgcataacg cgtttcatcg ggttgccaga
     6241 gcagacgcgt gctgatcggt aacaggtaat agcaggcaat aaacgcgaag aggccgataa
     6301 ggtaacgtac cgatttcatc agcgactacc cgcaatttgt tggcaaccca gccagccttc
     6361 acgtccggca atatttccgc gaacaacttg tccgagcggc aacgtctctg ataacagttc
     6421 gcttaaaggg caaaatgtca cgccttcctg agctgcgcgt ttgaggagat ccacaaaatt
     6481 atgctgataa gcgcagcctt caacttctgc atgaatggta taaaccggcg tgcctttatc
     6541 tcgcaggatg cggttgagta accaaccgtt aaaatcttct gctttcacat cccgaccaat
     6601 cacttcatcc caggtgggta aggtcaccgg aatttgcgca gtgccagggt ttcctgattc
     6661 gagcaatgga cggaacggca tggccccacg acaatcgctg ttgtagcgca aatggaacgc
     6721 ttcttttgct tcgatcacct tctggtcggc acgccagccc gcagcggcag aacaggttac
     6781 cggttgaccg ataatctctt ccagagtgcg aagaccgcgt gcaatatcgt cgatcattgt
     6841 ttgccgatcc cagttaccgc tacgggcttg ccaggcatgg tgatcccagg cgtgcaggcc
     6901 gacttcgtga tgtttagccg cttcacgaat gatatcggca ttggcatgac caatctcttt
     6961 acctggccag gccgtacctg ccagtaaaat atcccagcca taaagcgatg ccgcatttga
     7021 gcgcagcatc ttccacaaaa actgtggctt caccagtcgc cagagatggc ggcccatatt
     7081 gtccgggccg acgctgaaaa aaatgctggc ctgaatatta tgcttactca agatttccag
     7141 cagacgcggc acgccttcac gggtgccacg aaaggtatcg acatcaatgc gtaagcctac
     7201 tttggtcatg atggtttatc cgtaagatca acggtgcgca ggaagaaatc cagcgtttcg
     7261 tcgatggttt cctgcatatc aattttcggc tcccagtcca ggcagcggtg ggcattgcgg
     7321 atgctcggtt tacgatgctc tacgtcctga tatcctttgc cgtagtagct gctactttcg
     7381 acaacgcgaa agcccgcaaa cggtgggaaa tgatggcgca gcggatgttt ttcgaagctc
     7441 gccagcagca tctcgcccag ttcctcaatg ctcgcttcgt tctcaggatt gccaatgttg
     7501 ataatttcac cgtcgcagcg atttcccgca ttttcgataa tgcggtataa cgcctcgata
     7561 ccatcgcgaa tatcagtaaa gcagcgtttt tgttttccgc catcaatcag cttaatcggt
     7621 gaaccttcta ccagattgag aatgagttgc gtaatagcgc gggagctgcc gattcgtgcc
     7681 gcattaaggt tatccagtcg cggtcccatc cagttaaacg ggcggaagag ggtgaactgt
     7741 aaaccctctt tttcgccata ggcccagatc acccgatcaa gtaattgttt tgataccgaa
     7801 taaatccagc gtggtttatt caccgggccg acgattaaat tagaatggtc ctcatcgaag
     7861 tatttatcgc tacacatccc ataaacttct gaagttgacg ggaagatgat tcgcttacgg
     7921 tacttcacgc agtagcggat aatgcgcaga ttctcttcaa aatcgagttc aaatacgcgc
     7981 agcgggttgc gggtatattc aatcggcgtg gctatcgcca ccagcggcaa gacgacatca
     8041 cattttttga cgtgatactc aatccattcg gaatgaatac tgatatcgcc ttcaacaaag
     8101 tgaaaatgcg gatgattcag aaaacggctt atcgcatcac tgccaatatc cagaccgtaa
     8161 acttcataat gatcttcgcg cagcaggcgt tctgtcagat ggttgccaat aaagccattc
     8221 accccgagga tgagtacccg ggtacggcgt cgggcggtgc aggcaggctg gctattcaag
     8281 cgtgaacctt gcaccaggcc cagcgtctgc gctaattgcg agccctgcat agtaatgccg
     8341 tcgcccgcct gtccggtgac gatttccagc gcgccatcgc cacaggcaat cagcagtggc
     8401 gcaacagaaa tcacgctccc cggctgtgct ttgctggcat gaggatgaac acgcgacgac
     8461 cagacggtga atttctgatt gccaacatag ctgaaggcac ccggccacgg atcggcaacg
     8521 gcacgtacca tgttgtgcag tacggatgcc ggtttatgcc attcaaggaa actgtcatcc
     8581 ggcgttctgc gaccaaaaca ggtggcttcg ttttcgcgct gggcgatttc cagaatatta
     8641 ccgtgtttaa tggcgggtaa tgtctgttcc agtagctggc gcgcggcatg gcacaattta
     8701 tgatgcagcg taatagcgat atcgtctggc gcaatggcaa tgcgcagttg cgccacaatg
     8761 gccccggcat cggcacgttt caccattcgg tgcaatgtaa cgccagtttc cgtttcaccg
     8821 ttgaccagca cccagttcag cggcgcgcga ccacgatatt ttggtaacag cgaaccatgc
     8881 agattaaatg cacctgcggg agcgagctgc aaaatttcgt cgcaaataag atggcgataa
     8941 taaaaagaga aaatcacatc tggcgacagt tgggcaatgc gttccaccca cagcggatga
     9001 ttaacgtcat ccggcgcata aaccggaatg cctctttccg ctgccagacg agccaccgaa
     9061 ccataaaagg ctttttcacc gggattatcg gtatgggtaa aaatggcgct aatttcgtaa
     9121 ccggcagcca gcagagcttc aataccgagg catcccatat cgtggtaggc aaaaacgacg
     9181 gttttcatta ttcattttcc ttgctggatg gacggataac ttgctgaaca aaatagcggg
     9241 ggcgggcgcg gacatcggtg tagatcctgc cgatatattc accgagtaat cccatgccga
     9301 taaactgagc gccaataaaa gtaaacagca cggcaaatag cataaagacg ccttctgccg
     9361 cccattgtgg tccgaaggtc aggcgtaaaa tcaccagcag caccgcaatg ctaaaacctc
     9421 caatcgcaat aatgctgccg agcagactca gcatacgtag cggcgttgtg gtaaggcagg
     9481 tcaccaggtc gtacatcaaa ttaatcaggc gcataaaact gtatttggat tcaccaaact
     9541 cacgctcgga atgatgtact ggaatttcaa tggcacggcg ggcgaagata ttcgccagaa
     9601 tcgggataaa ggtgctacgt tcatggcagt gcaacatcgc atcgacaata tgacggcgat
     9661 aggcgcgcag catacaaccg tagtcaccca tcgctttgcc ggtggtgcgc tgaataagcc
     9721 ggttaatcat cttcgaagct gttttacgaa accagctgtc ctggcggttc tggcgtacag
     9781 tccctaccac gtcgtaacct tcatcggctt ttgccaccag gcgggggatt tcttctggcg
     9841 gattctggag atcggcatca agggtaataa ttaagtcgcc ggtaacgtga ctgaatcccg
     9901 ccataatcgc tgaatgttgc ccgtaattgc ggttaagcaa aatagacaca atatggctgt
     9961 tctccgcttg tgaggcttcg accagcatat gcgcggaatt atcgctactg ccgtcatcaa
    10021 tcagcaggat ctcatactct ttccccaacg attcacaggc tgtggtggtg cgcctgatta
    10081 attccggtaa gctttcctgc tcgttataaa cgggaataac caccgagact ttcttaacag
    10141 ggtggatttc aaacatggct tattgtcctg cgagttgctg aagggctgtg atgacacggt
    10201 cggcatcggc ggtagtcata tccgggaaca gcggcaaaga acagatgcgt tcgctattcc
    10261 attcggtatt cggtaacgac agcgtgggaa aacgctcgcg ataatatttt tgtgtgtgag
    10321 cggcgcggaa atgtaacccg gtaccaatgc ctctttcttt taacgcttcc atcaacgcat
    10381 cgcgactgat accacaacgt tgttcatcga cacgaataat aaacagatgc caggcgtgaa
    10441 cgtgcggcca ggcgggaagg cttaatggct gaaaggggag agctgccagt gcttgctgat
    10501 attgctgggc aatttcgcgc cgacgggtgt tgaggtgctc taattttgct aactgtgtca
    10561 gggcaatcgc ggcgttaata tcggtcagat tgtacttata gcccggtgtt aagacttcag
    10621 cctgcggtgc acggccccag gtttgtctgt cataggcatc gacacccaga ccgtgaaatt
    10681 tcagcatccg tagctggcgg gcaaggtttt cattatcagt tacaatcagg ccaccttccg
    10741 cacaggtaat atttttaatg gcatgaaatg aaaaaatagc ggtacctttt gcgccaatat
    10801 gtcgcccttt gtaatacgta ccgacggcat gggcagcatc ttcgataact gcgatgccgt
    10861 aacgttcgcc aatggcgcga atggcgtcaa tatctgctgg tgcaccggca taatgcaccg
    10921 gaatgatggc tttagtgcgt ggcgttatgg ctgactcgat cgcttcaggc gtgaccatca
    10981 gcgtatcgcg gtcgacatcc accattaccg gcgttgcacc caacaaggaa atcatattga
    11041 gggttgaaac ccaggtcagg gaaggcgtaa tcacttcatc gcccttgcca atttccaacg
    11101 ccattagcgt gatatgcatt ccggcggtgg ctgaactgac cgcgatggca tgctgatttc
    11161 ccgtcaactg gcaaaaggct tgctcaagcg cctgattctt cggaccggtt gtgatccaac
    11221 cggattcgag aacctcttta actgcagcga gttcctccac gcccattgct ggtcgcgaaa
    11281 aaggcaaaaa ttctgacatt gcttttcctt ccgccattga atacctgtcc acttatattt
    11341 gctataaaga gtgttgtgta tattttgcca tttgg
//
LOCUS       DAZGAN020000144        11195 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGAN020000144 DAZGAN020000000
VERSION     DAZGAN020000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.144"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1472)
                     /locus_tag="ACHKYO_RS21760"
                     /old_locus_tag="pgaptmp_004352"
     CDS             complement(<1..1472)
                     /locus_tag="ACHKYO_RS21760"
                     /old_locus_tag="pgaptmp_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU4431088.1"
                     /translation="MAGNFADLTAVLTLDSTRFSEEAARVKKELGETSDLADLMAGRV
                     SQSFKKQAAAVEQGLSRQALAAQKAGMSVGQYKAAMRMLPMQFTDVATQLAGGQNPWL
                     ILLQQGGQVKDSFGGMIPMFRGLAGAISLPMVGVTSLAVATGALAYAWYQGDSTLSAF
                     NKTLVLSGNQSGLTAERMLTLSRAGQAAGLTFNQAGESLAALVSAGVRGGEQFDAINQ
                     SVARFASASGVEVDKVAEAFGKLTTDPTSGLIAMARQFRNVTAEQIAYVAQLQRSGDE
                     AGALQAANEAATKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQE
                     MLIKAEAAFKKADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQD
                     KNAQQQSDTEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTL
                     MAAAKKDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQA"
     gene            complement(1462..1866)
                     /locus_tag="ACHKYO_RS21765"
                     /old_locus_tag="pgaptmp_004353"
     CDS             complement(1462..1866)
                     /locus_tag="ACHKYO_RS21765"
                     /old_locus_tag="pgaptmp_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4431089.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRREEAQTERPDEDDMLMRKAAGLSGGVRFGADGKEI
                     VTVSDDMRSSTEDEAMLMMVSEGIAGGVRYGG"
     gene            complement(1893..2324)
                     /locus_tag="ACHKYO_RS21770"
                     /old_locus_tag="pgaptmp_004354"
     CDS             complement(1893..2324)
                     /locus_tag="ACHKYO_RS21770"
                     /old_locus_tag="pgaptmp_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4431090.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTASPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            complement(2344..3093)
                     /locus_tag="ACHKYO_RS21775"
                     /old_locus_tag="pgaptmp_004355"
     CDS             complement(2344..3093)
                     /locus_tag="ACHKYO_RS21775"
                     /old_locus_tag="pgaptmp_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431091.1"
                     /translation="MATPNPLEPVKGSGTTLWVYTGTGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDQGDITP
                     VTGITVTPPTGNVAKGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            complement(3101..3499)
                     /gene="gpU"
                     /locus_tag="ACHKYO_RS21780"
                     /old_locus_tag="pgaptmp_004356"
     CDS             complement(3101..3499)
                     /gene="gpU"
                     /locus_tag="ACHKYO_RS21780"
                     /old_locus_tag="pgaptmp_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HGU4431092.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKITYEM"
     gene            complement(3512..4135)
                     /locus_tag="ACHKYO_RS21785"
                     /old_locus_tag="pgaptmp_004357"
     CDS             complement(3512..4135)
                     /locus_tag="ACHKYO_RS21785"
                     /old_locus_tag="pgaptmp_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431093.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRAAATTLNRVAESIIAKTASSV
                     ARELAVPRRLIRERIRLQRASADRVYAKVIINTGNLPAIKLGTASVLLSRRKRRKKGE
                     RSVTKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(4138..4419)
                     /locus_tag="ACHKYO_RS21790"
                     /old_locus_tag="pgaptmp_004358"
     CDS             complement(4138..4419)
                     /locus_tag="ACHKYO_RS21790"
                     /old_locus_tag="pgaptmp_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HGU4431094.1"
                     /translation="MSDLFTRMCCRMDVATVRMMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(4412..4738)
                     /locus_tag="ACHKYO_RS21795"
                     /old_locus_tag="pgaptmp_004359"
     CDS             complement(4412..4738)
                     /locus_tag="ACHKYO_RS21795"
                     /old_locus_tag="pgaptmp_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HGU4431095.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSAAV
                     AVKINV"
     gene            complement(4826..6850)
                     /locus_tag="ACHKYO_RS21800"
                     /old_locus_tag="pgaptmp_004360"
     CDS             complement(4826..6850)
                     /locus_tag="ACHKYO_RS21800"
                     /old_locus_tag="pgaptmp_004360"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGU4431096.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQTGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQALQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(6795..8297)
                     /locus_tag="ACHKYO_RS21805"
                     /old_locus_tag="pgaptmp_004361"
     CDS             complement(6795..8297)
                     /locus_tag="ACHKYO_RS21805"
                     /old_locus_tag="pgaptmp_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4431097.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            complement(8297..8509)
                     /locus_tag="ACHKYO_RS21810"
                     /old_locus_tag="pgaptmp_004362"
     CDS             complement(8297..8509)
                     /locus_tag="ACHKYO_RS21810"
                     /old_locus_tag="pgaptmp_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431098.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(8506..10629)
                     /locus_tag="ACHKYO_RS21815"
                     /old_locus_tag="pgaptmp_004363"
     CDS             complement(8506..10629)
                     /locus_tag="ACHKYO_RS21815"
                     /old_locus_tag="pgaptmp_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU4431099.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            complement(10626..11102)
                     /locus_tag="ACHKYO_RS21820"
                     /old_locus_tag="pgaptmp_004364"
     CDS             complement(10626..11102)
                     /locus_tag="ACHKYO_RS21820"
                     /old_locus_tag="pgaptmp_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HGU4431100.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGLLVSVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
BASE COUNT         2378 a         3538 c         2690 g         2589 t
ORIGIN      
        1 gcctgaagcg tcagcagggc agcatgagca ctgtcttcct gacgatcgcc cgcagacacc
       61 ttcacgccgg actgtttcgg ctttttcagc gtcgcttcat aatccttttt cgccgccgcc
      121 atcagcgtgt tgtaatccgc ctgcaggatt ttcccgtctt tcagtgcctt gttcagttct
      181 tcctgacggg cggtatattt ctccagcggc gtctgcagcc gttcgtaagc cttctgcgcc
      241 tcttcggtat atttcagccg tgacgcttcg gtatcgctct gctgctgcgc atttttgtcc
      301 tgttgagtct gctgctcagc cttctttcgg gcggcttcaa gcgcaagacg ggccttttca
      361 cgatcatccc agtaacgcgc ccgcgcttca tcgttaacaa aataatcatc cttgcgcaga
      421 ttccagatgt cgtctgcttt cttaaacgca gcctctgcct taatcagcat ctcctgcgcg
      481 gtatcaggac gaccaatatc cagcaccgca tcccacatgg atttgaatgc ccgcgcagtc
      541 ctgtctgccc aggtctccag cgtgcccatg ttctctttca ggcggcgggt ctggtcatca
      601 aaccctttcg tggcggcctc gttcgccgcc tgcaatgccc cggcttcatc gccggaacgc
      661 tgcaactgag caacatacgc aatctgctct gccgtcacgt tacggaactg gcgtgccatc
      721 gcaatcagcc ccgacgtcgg gtcggtggtc agttttccga aagcctctgc aaccttgtcc
      781 acctccacac cggaggcaga cgcaaaacgc gccacactct ggttaatggc atcaaactgt
      841 tcaccaccac gcacaccggc actgaccagg gctgccagtg actctcccgc ctggttaaac
      901 gtcagccctg ccgcctgccc ggctctggag agcgtcagca tgcgctctgc cgtcagtcct
      961 gactgattac cggaaagaac cagggtttta ttaaacgctg aaagcgtgga atccccctgg
     1021 taccaggcgt acgccagcgc accggtcgcc accgccagcg aggtgacccc gaccatcggc
     1081 aggctgatcg caccggcaag cccccggaac atggggatca tcccgccgaa tgagtccttc
     1141 acctgaccgc cctgttgcag caggatgagc cagggattct gaccaccggc aagctgcgtg
     1201 gccacgtcgg tgaactgcat gggtagcata cgcatggccg ctttatactg cccgacggac
     1261 atcccggctt tttgtgcagc cagggcctga cggctcagcc cctgttcaac ggcagcggcc
     1321 tgtttcttaa aagactggct gacacgcccg gccatcaaat ccgcaaggtc actggtttca
     1381 cccagttctt tctttacccg tgcagcctct tcagaaaaac gggttgaatc cagtgtaaga
     1441 acagctgtca gatcggcaaa attacccgcc atagcgtaca cctcctgcaa ttccctcaga
     1501 caccatcatc agcatggctt catcctctgt gctgctccgc atgtcatcac tgaccgtaac
     1561 gatttccttc ccgtcagccc caaagcggac accaccagaa agtcctgccg ctttccgcat
     1621 cagcatatcg tcctcatccg gcctctccgt ctgcgcttcc tcacgccggg ggacaagcag
     1681 actgaaatcc gagggatgca tatccggatc gcaaaaaaac aggctgagta cggcgtacgt
     1741 cagcccggaa aaatgcatat ccagctgagt atcgtgaaaa taatgcgtgc ggtaaaaacg
     1801 gtgccagtcg gcatattcgg tggatgtcat cccggcaagc atggcgcgcc agtcgggtct
     1861 ccccatctca cgcgccagtc tgagggcaaa gttcagctcg ccgtcgaaga ctttcccgca
     1921 gaaaaatcat catcagtcag cgcgttattt ttcgccactt cagtaatatc agtatccgga
     1981 cgaacagctt cgatcatccc ggacaggcac aacacaacgt cttccgcccg ggcaatggca
     2041 tcggcaggcc aggtggtgag cacttcctgc tctatcttca tcacggcctc attcattgac
     2101 ggtgacgccg ttttctgtgg atggttatgc cacagggaca tcgccaccag aaacgccccg
     2161 gttctgacaa ggtcctccac gctcacctgc aggttaccgc tggcttcagc ctcttctgcc
     2221 cgccgtttca ggagggcaag atgctcaata cgctgcagcg cagacagctc agaaagcgtg
     2281 acagacacac cgttatattc aaattgttct gttttcagaa acatgcttta tcactccctc
     2341 agcttaaccc gctgcaccat ccgtgacggt aatttccgcc accgccgcaa actcaccatt
     2401 gccggtgacc acagggatct gtgctttacc tgccgcaaca cctttcaccg tgatcgtgtt
     2461 ccccttcacg gtaatggtgg cgaaattctg attcgccgac gtggcacgga aggttttatc
     2521 cgtcgcgcct tccggctgaa cggccacggt cagggtgata ttctgacctt ttgccacatt
     2581 gcccgttggt ggcgtcacgg taataccggt gaccggtgtg atgtccccct gatcttccgc
     2641 cagcgacgga cgaccgatat tggtgatttt taccgtacgg gtgatcacct ctttggcggt
     2701 caccgcttta ccaatggcgc tcacccagcc acggaacaca tccaccgtac cgttcgggaa
     2761 gcgaattttg taggcccggg tctcactgct gtcaaaccag gcaatcaaat cgcgctgccc
     2821 tttctcaccc ggcttccagg ccagcgtaaa actggtgtca ccggcagatt tctgcccctg
     2881 cccggtggat acccagtcag catcctcatc atccagatag ttatcatcgt aggattccgc
     2941 cgtcatctcg ccgggggtca gatccttcac cttcgccagt cgctgccagt catcgtctga
     3001 caacgggttt gcataagcat caccagtgcc ggtgtacacc cacagtgtgg taccggaacc
     3061 tttcaccggc tcaagaggat ttggtgttgc catatcgtcc tcacatctcg taggtaattt
     3121 tccacaggag atctgccgat ccccacatca taaactcatc atcccggcgg tagtcatacc
     3181 cctgaagatt catcttcagc agtaatgcac tgagcccggg aactgcctcc agcgcaggaa
     3241 gaattttttc ttccatccac atatccagtg ccgagtccgg ttcttttgcc ctgagaaaaa
     3301 cctcaatatg cagtgtcgcc tcccaggttc cctcatcaac gaactcgtca gcagcagacg
     3361 catctgtcag gtaaacagca acagcaggca gttcctgttc atcaataaaa accgggcgac
     3421 cgtcaaacca gctcaccctc tcagaaatat tttctttcag ggcagacaga actgccgccc
     3481 gtatttcacg gtgtttcata caccctccca gtcattttct tttcagcacc aggcgtaact
     3541 gatgcgtcat ggctttcatc atctgcgccg gtaatttttc ccggtacatc cggtcccgtt
     3601 cacgttcaaa ggtttctgcc agcggtccgg cagtcggaat cttcaccact tcgatcggca
     3661 gacggtggcg tttcggcctc cctttgctgt cagcgccggt ggacgatgat gcccacggca
     3721 tacgctgcat cacatgccag cgtccgttag ccagccgggt gataaaggca tccgggatcc
     3781 gtcttttccc cacaatcagc acgctgccac cgcctttcgt gaccgaacgc tcgcctttct
     3841 ttcgtcgctt tctgcgggaa agcagcacgc tggccgtccc cagttttatg gcgggcagat
     3901 taccggtgtt gatgatgacc ttcgcataaa ccctgtctgc gctggcccgt tgcaggcgga
     3961 tacgctcacg gatgagacgg cgcggaaccg ccagctccct ggcaaccgaa gaggccgttt
     4021 tcgcgatgat ggactccgcc acacggttaa gtgtcgtggc ggcagcccgg ggaacggcac
     4081 ggcggtcaat tgcatccaga tttttcatgg cctgcgccag accttttatt gccatactca
     4141 ttcctgttcg acaaaaatcc ggggtttacc gttgtacgtg tcataacggg tcaccgtcag
     4201 tgtgcgcccc gcaaacacaa caacatcatg acgggccgga cggtaccggg ctgaaaacac
     4261 caccagtgac aactggctgc ccgaaagcgc ccccatctcc gcggactctt cctccggcat
     4321 cacgtcgtac acgacgccgt taatctccgc ctgtttgccc atcatccgaa cggtcgccac
     4381 gtccatccgg caacacattc gcgtaaacag atcagacatt gatttttacc gccacagcgg
     4441 cgctgtttgc aggagcattt tcccaggcta ctcccgctgc caccgcaccg tctgcagcca
     4501 gctgcacaac cccgtccttc agataaacca ccgcgccgga ctgaatgtcg tcagcagact
     4561 gtttgggcag caggaacacg ccttcggcaa aaccgtcacc ggcctcaccg gcaggaatat
     4621 cggtaatggc cacggccacc atactgccga ccaccaccgc agcaccgctc aggatggtct
     4681 gatctccggc attcaccagt tcaatggtgg taccgtcctg tacaaaattt ttcgccataa
     4741 tgctgtttct ccggacagcc cctgtggggc tgtttttcag gcataaaaaa agccctttcg
     4801 ggcagtgatt gtgataacgc ggttatcagg ccaccgacga acgcaccagc ccgcgccagt
     4861 caagtggtgc cactccggca tcaatacgga tttttgtggc aataccgtca gtggtgaaac
     4921 cttcctgctg atcaatgtat ggcgtgtcca caccatccag ccaggccact tcaatggtgt
     4981 cagtgccctg tgccgccgcc agataccagg ttttcggatc tgccgcatca agacgcgctt
     5041 ctgcaatcac ctcagcaaag ttctggatgg ggttaatgac accggcgttt gcatccgccc
     5101 ctttcacact ggccgatttg atggtctggt tcgccaccgt ctccagtgcc accggtacca
     5161 gcataaaggc cggacggata ttcagggcgc gatcgccttc tttctgcagg cgcatcatct
     5221 gacgggccgc atccagtccg gaaacggaga tccccccggt ggcaatattt ttgtgatcgg
     5281 catggaacag cgccttaccg tcagacagtt tcgggttatc cgtcagcacc ttgtaaacca
     5341 ggtcaccaat cgttgccttc gccgcacgcc ccatcttcat cggcacgtcc accagcatat
     5401 tcagatcatc attgataatg gcctggcggg tgatggagaa aatctccccg taggtggcca
     5461 gagcaatggt ctccttgcga tctgaggtgg tgatgtattt atactccgcc ccctcacgaa
     5521 cctggcgcag agaaccaaaa ccgcccatcc ccacgcgata cgctgttttg aagtctgaca
     5581 ggcgtccctt acgggtccac ttctggaagg tttcttctga ttcctcccag ccctggatca
     5641 gccccttgtt cgacacatcc agcagaatat tgccaaaatc agaggtgctg tgcgtcagcg
     5701 ccagcccgac catctgcatg gggttataac tggccacccc aataccgcgc tccgtcagtg
     5761 acatacgagc ccattcacgc agggtcatcc cgttataggc gttatccttc tcgacatttt
     5821 caaatccggc acgggccagc atcgcctggc ggatcccgtc tcccacaaaa ttgccgtttc
     5881 cggcataaat atgggccggt gtgtttttgt tggtcggcga tgactccttg cccatttcat
     5941 tcagaaggcg ctcgcgggcc atttccagcg aacagtcagg atcggccaca cactgcgcct
     6001 gaagcgcctg atagcgaccg ccaaacatgg caaacagatc gttaatgcct gacatgcggg
     6061 ctttctgctc agccataacg cgggcgcgaa tggtcgcctc atcagacact gccggtaccg
     6121 gtgatggttc tgttaccgcc ggtgcaggga ttgtcactgt ggtatcacgc ggggcactgt
     6181 tgcgtggcgg agtaatcatg tttcggatgg attccggcat ctttttaaat tcctctgtac
     6241 gttttgactg aatacatgcc attgcctcaa cagcgggtgt cacctggtca gcaaatccgt
     6301 gtgccagaca ttcggcaccg gacatccagg tttcatccgc cagcatggcg gcaatttcat
     6361 cggtggtttt tccggttttc tgcgcatagg ctggcaacag taccgattcg actttatcca
     6421 gcaaatcggc ataactgcgc atatcctcag catccccgcc actgaatccc catggcttat
     6481 ggatcatcat gaaggcattt tccggcataa tgaccgtatc accggccatc gcaatcacag
     6541 atgccatcga ggcggcaacg ccatccacat acacggtaat ggtcgccccc tgatttttca
     6601 gggcattaaa aatggcgatg ccttcaaaga catcgccacc cggtgaattg atatggagat
     6661 taatgtgggt gatatcaccc agtgcattca gttcgctgac aaactgcttc gcggtaactc
     6721 cccagaaacc aatctcgtca taaatataaa tatccgcgtc acccggcccc ccagtctgca
     6781 tcctgaacca ggatttattc ttcatgctgg ctttcggtgt cgcgctgata ctgtcgttca
     6841 gttccggcac tgttgcctcc tttgtcgttg acggggtcag tatcaaagac cagccccagt
     6901 ctgctgtttt catcaatttc agccttgcgg cgacgtttga cctcatccgg attgcgcccg
     6961 ccggcacgca cccagtcaga ttctgtcgct gcaccacccc ggatctgaat tctccaagct
     7021 tcagcttcct taaccgggtc gatccacggc atcaccggac cggaatacgt cgcgttatat
     7081 agcgttttca tctccacatc cgccggaatt ttcagcagac ctgccgcaac caccatattc
     7141 agccatgtcc ggtacaccgg gcgggttacc gcgccaataa aacagtcctg caggatcagg
     7201 taaccatccg tggactcgac cagctcctgc cgctgggcgc tgtaggtgcc gttatagtta
     7261 cgcgccgcac tggaaaaact cagacgactg cctgctgcca ctgcacgcaa ctggccgttg
     7321 cggaaagttt caagattggg attgggacgg tcagatttga ccatgccgat atcctcgccc
     7381 ttgcgcaaat cgtcataaat aatacccggg gtgatatgga cttcccgctc ggtttctttg
     7441 atccccggat cttcatagtc ctgtccgtca cctttacgga tatacagtcc cagcgccgca
     7501 gcaatacgcg ccgctgtcag ttccgcatcc tcatactcct taagggcact gatccgcatc
     7561 agcacccccg ataacatgga tgagcctcgc gtctgatgca gacgacgagt gaacttcagg
     7621 tggatcattt ttccggcagc gatttctttc gtatcactct gccgaccgct gaccggataa
     7681 tttttataaa ccagatattt tttcggtctt ccccactcat caagaaaaac cccctgattc
     7741 agtccggcgg attcatctgt gcgcatggga acaaaatccg gctccatcgc ctcaagccag
     7801 aatggcactc ccgccgtccg ttccagaccg tttcccgcac cactgaccat ctgcgcaaac
     7861 acttcaccat cccgcagcca ggtccgcagc agtaaacgtt caagcacagg acgggtatac
     7921 tgccctgtca catccggact cacggaccat tcagcccaca aacggcggat atccgcagcc
     7981 agctcagccg ccatttcccc gttttttcgt aatggctgag gctccacaat aattcccctg
     8041 gcaccaatca cccgctcttc cagcttgtca aacacaccaa tcaccaggtc atgattgata
     8101 tcaagaaaac gggcctgctc ccgcagggaa accgcaccgt atttactgag ctgatcggca
     8161 gagcgatttt cccgccgggc tttatgtgtc cgggtcggtt tcaccgcctc ataggccatg
     8221 attaacgccc ttgaacgcag tctggctgct ttccacccgg gggaaaacac gcctatcaca
     8281 tcatcaataa ttgccattaa aacctcgcca gtttaaatcc tggttttccc cgcctgcggc
     8341 tcaccatcgc ggcaagcctg cgttcccact cctgacgtcc ggcgcggatc tgagaaaggc
     8401 tttccagcgt cagttgctgc ccgttgaagg tgacagactt cccctccagt acggccattt
     8461 ccgcttcacg gtaccgctgt atcatttctc tggcttcttc tgtgctcaca accagcctcc
     8521 tgatgttatc catggattat cttccgcacg ctccgtacgc agttttttct tccggcgacg
     8581 gcgtttttct gccccggccg tcagttccgg ggataccgtt tcaccagaac gctcctgcgg
     8641 gaagacgagc cacgtttccc gctgtgccca gtccggtgcg gagggccagc ggatcttttc
     8701 gtaaccatgc agaacggcaa gcgcatccgc ataaaccagc aggtcaaacg cctcgttagc
     8761 gcccctgccc ggttttcgcc attttccgtc actgccgcgc tcttcatagg tcagctcatc
     8821 gtaaaaccac cgccccagcc agtcgggaaa gtggatatag ttcggccctg gtgtgtcacg
     8881 ccacagggca ttatttacac ggtccttaaa cgcatccgtc tgaaccagcc acagcgcgac
     8941 atcgccactg gctctggcac ggcgggcact tctgccggta ttatccggga aggtacggtt
     9001 aatcagcctg tcacggcgaa gaccatcccc cttgaacaga aacaccctgt tgcccagtcc
     9061 gtcactccgg caacgacgcc agaaacgata ggcgttatct gtcaccccgg cttcccctcc
     9121 cgtatccacc gccatggcca tcagacgcat gcgcacatcc ggatcagaag ccagcggcca
     9181 tgttttatgg aacacatccg tcagcaacaa atcccagtcc tccggatatg ccgccggatc
     9241 aaccggcaga ctttcaccgt tgggactgca gcgcagtgaa tgccggatgt tgtagcgatc
     9301 aacaatccag cgttccccct gctctccgta tccggtgatc tgcacaacaa aacggcgatt
     9361 tttaccgccc tgtacgtcaa ccgttgcctc aataaaacgc acaccatccg gcacagatcg
     9421 ccggggaaac ggctcggcac gctgttcaag cagttcactt ttacgctgtt ccgtggctga
     9481 acggggcaga tagggtcgtc cgatatcggt gttcaccacc gctttcaggg tctcttcact
     9541 gccggttcgc tcatactctt cttctgccgc cagcagttta aaaatcagtt gttcccaggt
     9601 ctgaaacgcc gcagccggcc cctccatcca aaatgacgca atccgggaat ttcgtggcgt
     9661 tccggtgata ctgccgtccg ccgccgcccg ttcaccttca cgaagccaga tcccctggtt
     9721 attcagttca cgcttctgtt ccggagcaat caggccgcgg cagtgcggac acatcaggcg
     9781 ggccgcctgc ccggcagcca caaaatcagg gttattccgg taaccggtca tgttatccat
     9841 caccggctga aaatattccc cgcagtgcgg acacggccag taccaccggc ggcggtctcc
     9901 ccggttatac agtgacagga tccccgttgt tggcggtgcc tcatgtgcgc cgccacagcg
     9961 ccatttggtg tcagtgatat cccgcccggg cgaactttcg accagggtca tccccgagga
    10021 cataaaggtg gtggtacgct ttgaggccag ggtgaaggca tccccttcac cgtcgacgtt
    10081 ctcaggaaaa cggtcataat ccgtcagcgc cacacgacgg taatccgaag aggaaaatac
    10141 agtgatcgac ggccagccaa ttttcaggaa tgagccgtca agaaacattt tgtcgtggac
    10201 gttgttgtca ttacgggaag ggctgaggcg cttactgacc tccggactgt ggcgaaacgt
    10261 cctggaaaga cgcgttctgg aatgctcacg cgcctttgtc tcggtcatct gcaccaccag
    10321 catatccgcc ggatcacaga tgatgccgta cacaatccag ccatcaatca gcccttcggt
    10381 tttcccggtt cgcgcaggtc ccacaaacac caccgcgtca tattcacggg ctgataatgt
    10441 attaatgggg tcaatcatat agggcgtcag cgatgactcc cacggaccgg aagtattggc
    10501 tccccgtggt acccgcatat aacgcctgat ggcttccgct actggtaacc ggccaggtgg
    10561 gcgaaacagc gaggccactt cgcgccagat atcggatgcg cggctatggc tctcgttcac
    10621 ctgattcaca tatcggcctc atcacaacag tcaatgactg ccttttccag tgtgtcgcgg
    10681 atctcatcaa ccacaatctg tacttcattc agttgtgatg cggtccaccc cctgtccctc
    10741 tccagccggt caggccaggt ttccagtacc tgaactatcg ctttcaccac gacagaaaac
    10801 gaccgcctga catcactgac tgacacgagc agaccggttt cctgctcaaa tttcagtcgg
    10861 tcacgttctg actggtacca tgctttacgc gcatgaggat ccatttcctc gttatctaca
    10921 ggcagaggag ctttcatcag ctcggcaagg atatctgtca gtcggtacag tttgagatta
    10981 ctctcatgac caccggctgg gcttatgttt tttacccgag ccgcgacagt ctgtcgatga
    11041 gcaccggata atgcagccag ttgggaaata ttcagatgca gattttttaa ttcacgatcc
    11101 ataacttccc ctgaaaatta tgtaaataca cgccagtgat gaacaaaaaa caatcaactt
    11161 cgacactaaa aatttttatt atggtatata tcaat
//
LOCUS       DAZGAN020000145        11020 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGAN020000145 DAZGAN020000000
VERSION     DAZGAN020000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.145"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1644..1838)
                     /locus_tag="ACHKYO_RS21825"
                     /old_locus_tag="pgaptmp_004365"
     CDS             complement(1644..1838)
                     /locus_tag="ACHKYO_RS21825"
                     /old_locus_tag="pgaptmp_004365"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HGU4431101.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAK
                     GENIGWCIARAEYCKKTGRF"
     gene            complement(2096..2563)
                     /locus_tag="ACHKYO_RS21830"
                     /old_locus_tag="pgaptmp_004366"
     CDS             complement(2096..2563)
                     /locus_tag="ACHKYO_RS21830"
                     /old_locus_tag="pgaptmp_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431102.1"
                     /translation="MRDKTREAMRFFLGGRCYTAENLEKDYLAEVANYSDDRWEAPQR
                     AARLAAAVKRYKTSEMLRFIFATTAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRIYQARIKAS"
     gene            2723..2977
                     /locus_tag="ACHKYO_RS21835"
                     /old_locus_tag="pgaptmp_004367"
     CDS             2723..2977
                     /locus_tag="ACHKYO_RS21835"
                     /old_locus_tag="pgaptmp_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HGU4431103.1"
                     /translation="MKITTLSSRELNQDVTRAKRATNYGPVFITDRGKTAHVLLSIEE
                     YRKLTQQRRTLIDILAMPDADAVEFEPPRITVSLKPAEFL"
     gene            2977..3393
                     /locus_tag="ACHKYO_RS21840"
                     /old_locus_tag="pgaptmp_004368"
     CDS             2977..3393
                     /locus_tag="ACHKYO_RS21840"
                     /old_locus_tag="pgaptmp_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4431104.1"
                     /translation="MYILDTNVISELRKARTGRIDNNVAKWALHTDAADFFISVISIM
                     ELEQGILQVERRDMRQGIMLRSWMDQHVLPAFSDKILPVDTAVALRCSRLHVPDRRSE
                     RDALIAATALVHGMTVVTRNVGDFELTGVSIINPWA"
     gene            4291..4653
                     /locus_tag="ACHKYO_RS21845"
                     /old_locus_tag="pgaptmp_004369"
                     /pseudo
     CDS             4291..4653
                     /locus_tag="ACHKYO_RS21845"
                     /old_locus_tag="pgaptmp_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            4657..5012
                     /locus_tag="ACHKYO_RS21850"
                     /old_locus_tag="pgaptmp_004370"
                     /pseudo
     CDS             4657..5012
                     /locus_tag="ACHKYO_RS21850"
                     /old_locus_tag="pgaptmp_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
     gene            5158..6297
                     /locus_tag="ACHKYO_RS21855"
                     /old_locus_tag="pgaptmp_004371"
     CDS             5158..6297
                     /locus_tag="ACHKYO_RS21855"
                     /old_locus_tag="pgaptmp_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HGU4431105.1"
                     /translation="MNKIHANYYLQDSTARQIVQRTMGIIPYSVNVMNEHGMIIASGE
                     ASRLHQRHEGAVLALTENRVVEIDSATANKLKGVKPGINLPINFRDQLIGVLGITGEP
                     GEVKAYAQLVKMASELIIEQMALLEQKQWDKRYREELINQLILRDPPPDSLNAVVSWL
                     GINLQLPRVVVIMELQQPDRDALHNLMEYFEYRARDHLVTFSDFNDLIILKPISLKNG
                     VWDKSLELSDLQRLKHYAQSCGFSRLIVGGYFSGENGLHRSFQTAKSTQSMAKRLKLK
                     HQFIFHDDYALASLLDGLSGTWQAEELSKIWRNLVAKDTKGMLQKTLQNYFDHNCDLS
                     QTATGLHIHVNTLRYRLQRVEDITGIQINNLKQLFWLYIGMELQR"
     gene            6417..7685
                     /locus_tag="ACHKYO_RS21860"
                     /old_locus_tag="pgaptmp_004372"
     CDS             6417..7685
                     /locus_tag="ACHKYO_RS21860"
                     /old_locus_tag="pgaptmp_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGU4431106.1"
                     /translation="MVSITATGAIIALVVAIVLILRKVSPAYGMIVGALVGGLVGGAD
                     LVQTVTLMVTGAQGITNAVLRILAAGVLAGVLIESGAANTIAETVVKKVGESRALLAL
                     AIATMILTAVGVFIDVAVITVAPIALSIARRAGLSKLAILLAMIGGGKAGNVMSPNPN
                     AIAVSDTFHVPLTSVMMTGIIPGLFGLIVAYFLAKCLSQKGLMVMPDEMISHDNHGKQ
                     PGFLAAISAPLVAIVLLALRPVAGIVIDPLIALPVGGLAGAILMGRFKQSNAFMMSGL
                     TRMAPVAIMLLGTGTLAGIIANSELKDVLIHGLTSSGLPSYLLAPVSGAIMSMATAST
                     TAGTAVAAGVFSQTLLGLGVSALAGSAMIHAGATVLDHLPHGSFFHATGGSVGMLIHE
                     RLKLLPYETLVGFVVALISTLMFGVFGLAG"
     gene            7693..8838
                     /locus_tag="ACHKYO_RS21865"
                     /old_locus_tag="pgaptmp_004373"
     CDS             7693..8838
                     /locus_tag="ACHKYO_RS21865"
                     /old_locus_tag="pgaptmp_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HGU4431107.1"
                     /translation="MKIVIAPDSFKESLSAMQVATAIEQGFREIYPEAEYVKLPMADG
                     GEGTVESMVEATEGHYLFQNVTGPLGTPVQARWGMLGDGKTAIVEMASASGLHHVPPE
                     CRNPMLTTSYGTGELILAALDHGVERIILGIGGSATNDGGAGMLQALGVAMLDTNGKS
                     LPFGGGALSNLALIDASGCDARLKNVSVTVACDVNNPLCGATGASAIFGPQKGATPEM
                     VASLEAALTNFGIRLQDATGRNVMTTPGAGAAGGMGAALLGILNAELKPGVEIVIDAL
                     HLDSLVADASLVITGEGRLDSQSISGKTPIGVARCAKRYQKPVIALAGGLTTDHQIVH
                     EHGLDAVFSVLTQIATLPEALQDAEYNLRVSARNIASVWKLSQQQMR"
     gene            9306..9452
                     /locus_tag="ACHKYO_RS21870"
                     /old_locus_tag="pgaptmp_004374"
     CDS             9306..9452
                     /locus_tag="ACHKYO_RS21870"
                     /old_locus_tag="pgaptmp_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431108.1"
                     /translation="MAAINKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYR
                     GMSM"
     gene            complement(9523..10746)
                     /locus_tag="ACHKYO_RS21875"
                     /old_locus_tag="pgaptmp_004375"
     CDS             complement(9523..10746)
                     /locus_tag="ACHKYO_RS21875"
                     /old_locus_tag="pgaptmp_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGU4431109.1"
                     /translation="MNWQKKKIERSIKYQTRVFNEHINELVITRRNGKVITVNDGREM
                     IEFVSCSYLGLETHENLKHAIANAIERFGVQLSVARTRVKVDLFEQLEKRLNKIMNGA
                     HTLTFNAVTPCHLAVLPLLASGTLPNYKFNKKPYFILEKTAHATLQINRGLLQQFGET
                     IRVDFQHKNDIEEAFKYASINNMVPISISDSVGSMGGVAPVEFIVRMANRFDGYAYID
                     DAHGMSIYGENGSGYVMSCLDNKLDDRIIIAGSLSKAFGSHGGFIACNYPDTINFIKT
                     YGTTYAFGGPPSLPGIAACIAAADLHLDGTVSLRQNDLQQVIKYFDDKFNETEVINKG
                     TTMPIRGLLIGNEEKAITICKKMHDRGFATTVAMYPTVEEGKAILRCAISAIHTRENI
                     DSLHKNFIECINEQG"
     gene            complement(10743..>11020)
                     /locus_tag="ACHKYO_RS21880"
                     /old_locus_tag="pgaptmp_004376"
     CDS             complement(10743..>11020)
                     /locus_tag="ACHKYO_RS21880"
                     /old_locus_tag="pgaptmp_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4431110.1"
                     /translation="ICVIGAMIFNLTLGAYYPIANFISLQINGSRTGLAASITGFTQT
                     VSAGTISFLSVKISDNGVFLGTTLSISCLILALLSMFVTIIIGNNKK"
BASE COUNT         2815 a         2642 c         2617 g         2946 t
ORIGIN      
        1 ttaaaaagca aatcagaaaa aagttggttt tgcgtggggg gtgggcatcc ggggcatgag
       61 aacagactcg cgtttttctg gagaaactgc ggggattttt gattaaacaa tactcaccgc
      121 agagcggaat tttatgcaac gccggctgtg cggcacaggg atttttaatc cccagcatgt
      181 cagagggtgc ggatgacggt tcccgtctgg cgttttcatg ggcgccggtt gtcaaaaaat
      241 accagcgtga tcggtcgaag gataagcgca gattaatcag tgatgattga gcgcgaatct
      301 gacaacgagt atagcgacac agcaacaaaa aagacgccag aaacgcgctc agaggctttg
      361 taacgcgatt ttttacatga ggcaatggtt tcgctttgag gcgttcagcc gtgttcaggg
      421 cgcgattctg gtacatacct gactgtaatc atccctacga actactcttt gcaccagaaa
      481 cgcgcaggag tgttttagcg tgtctccggc gtgatgtcga gttgtgcggt aatgagtgag
      541 cgaaaaaaat cccctggatt acagggggaa cccatgatca gactttgaag tttatttgtt
      601 atttgatgga atgagcacga atcccggaga tgacgttcag gtggcaggaa acgcgctcag
      661 aagctttcta acgcactttt ttgcatctgg caatgggttt catatgtttt ggtgggttca
      721 ggctgtcaga gacgttttga gcgcgtttct ggcgcttgct gctactgcca aaccatcccc
      781 atggccagtt gatgccagaa acgcgcagga attttgtagc gcgtctccgg tccctgatca
      841 atgaattccc tgtcattaac atgcgtttac ccggaaacgc gccctgatgt tctatcgtat
      901 cgcgggaacg gtgtcgaggg tgtcggggca acgccctgac caggtggtaa tcgtatagcc
      961 gtgcgtgcgc ggttatacga ttacacatcc tgtcccggaa tcaggattgt ttttcgcgca
     1021 caactgcggc aaattcatcc cgttgcggtt gcaacgatgg gtatatcaaa actacgaaaa
     1081 ttattcaggc atttgctacc ccccttgccc tggcctccac cagtcacggg ggattttttc
     1141 gcctgtcctg attgtctttt gccgatggtt acggcagatt ggaaaagagc gcatttgtgg
     1201 tgctctctcc aaaataatat ttctcattac tcaaactctg atggcttccc cctgtaatcc
     1261 agggggattt tttaatttat cgctatgctt cgtaattcat tcagaataca gacagtatgg
     1321 ttaatcagtt tttcatcatt ttttcggtgt ttgccctgtc ctgcatcaga acgctaaaaa
     1381 aacaggattg gaatctcttt atgacgaatc ctccatttca ggatttttta tgtaacgtct
     1441 actacatgcg atccgttggt gaaccgttag ttatatattg gttatatgtt agttatatct
     1501 tagttatact tttgctgcgc gtcagttaca gaggcgtttt gagggtaaat tctggaggca
     1561 cgtgaccgac tgtatacaaa atgctggcgc attttgatac gtcgacgtgc caaaggggac
     1621 gcccctttga aaccccgcca gaattaaaag cggcctgtct ttttacagta ttcagctctg
     1681 gctatacacc aacctatatt ttctcctttg gcataatgaa gcgcatcatt ccattttttc
     1741 catgctccaa tgtaatcacc ttcacgctcc agtctgacag cttcgtttgc gggtgaaaaa
     1801 tatttatttt ctaaataaat tgctttgtcc tgattcatat tcgtaattta tatttttatt
     1861 tgaattaaaa aagagcgagc ccatcgggtt aatacatatt ggcatgactg ttgatatatc
     1921 agtccaacag cacgctcgta atcgttttaa cgtgattttc gcaacatggc aatccgggtt
     1981 catctgtccg gtaatcaata aaaaccgtgc gaaaaacggg acaggatgtg gccattaatg
     2041 ctaatgtatg catacattag cattaatggc tctgacaaat accaggcatc cagtctcagg
     2101 atgccttgat tcttgcctgg tagatgcgct ttacccgttc aatttctgcc agcgttgcgg
     2161 accagtgtag ttcggcggca tgacgatatc tggcagccat tttttccacc atctccggat
     2221 tgctgtccgc gctgcggtgc tgacgcccct tctccccaaa aacttcaaca accagccact
     2281 ggcttcctgt tctgccgaac aatgccttgc acaaacgtct cacagtcagc ggggttaaat
     2341 ctggatcggg atcgtaggca gtggtagcaa aaataaagcg cagcatttct gatgtcttgt
     2401 aacgctttac ggcagctgcc agacgtgcgg cgcgttgggg tgcttcccag cggtcgtcac
     2461 tgtaattagc aacttcagcc agataatcct tttcaaggtt ttcagccgtg taacacctgc
     2521 cgcctaaaaa aaaccgcatg gcctccctgg ttttgtctct cacatatcat ccctcattaa
     2581 tgctaatgta tgcatacatt agcattaata tctgacagca acaagcggcg aagactaccc
     2641 ctaatcaagt acatttcttg cattgtacta tttcatgccc tacaatgtag taacgtacct
     2701 atatacatca aaaggcaaca gcatgaagat tacaacccta tccagtcggg aacttaatca
     2761 agatgtaact cgagccaaac gcgccacgaa ttatggtcca gtatttatta ctgatcgggg
     2821 taaaaccgca catgtacttc ttagtatcga agaataccga aaattgacgc aacaacgacg
     2881 aactctcatt gacattctgg cgatgcccga tgctgatgca gttgaatttg aaccgcctcg
     2941 tattacggtt agtttaaaac ctgcggagtt tttatagtgt atattcttga tactaatgtt
     3001 atctctgagc tacggaaggc tcggacagga cgtatagata ataatgttgc aaaatgggca
     3061 ttgcatacag atgcggcaga tttctttatt tctgttattt ccataatgga acttgaacag
     3121 ggaatattac aagttgaacg tcgagatatg cgacaaggga ttatgctacg ttcttggatg
     3181 gatcagcatg tgttaccagc gttttctgat aagatacttc ctgtagatac tgcggttgca
     3241 ttacgttgtt ctcgtttaca tgttcccgac aggcgcagtg aacgagacgc attgatcgca
     3301 gcaacggcgc tggtacatgg gatgacggtt gtaaccagaa atgttggtga ttttgaatta
     3361 accggagtat caattattaa tccctgggct tgatatcgct tcccttcttc taggggtccg
     3421 cttggaaaac ggaaattttc ccacgctaag agaattgagt tgaacgagtg gttacatcca
     3481 agccagctaa ctcactgaac gtaaaggctg tacgaaatgt tccgaccgtc cggattcctg
     3541 acagtcattt ggctaaaaaa tagtcttaac gtggtttatt ttccgaattc caggctgacc
     3601 ccataaaagc atccttcagt tcagcgtcga ttcttaaact catattaacc tgtgccattc
     3661 atcacctcgt cattaccacg tatatacaca gtatataatt attcagatat cgtcaccatg
     3721 atatgccgcg ccagcaacat ggaaggcggc actccatgct gtatatggca gaggagtaaa
     3781 accgccgaat cccggcgtaa gccggtacag tcgtccgaat gtcagtagtt ctactccccc
     3841 ctgcgaggcc agtgctgacg cgggacatgg aacgggtgct ggagcgaagt ggggaagctc
     3901 cggtatactg gccaaaccta cacgcctcct tccgtctccg ggaagatgct ggcttcgtac
     3961 cctgcgtgcg tctgatctgt agctggccgt ggcacggcta cggacagatt ctgctccgca
     4021 acagtatcgg cgtgcctgac ctcctgaaag tcccgtaacg cattttctca cctcccatgc
     4081 cctgacgcta ttgagcgcgt tacagaacaa tttaagtcat ttaacgagcc tccaacggct
     4141 cgatcgaaat aaatcaaatc gtaatcgtca acgatatatg agaggagacc ccgctcgccg
     4201 caaatcgatg accgaacgta gtgagcgaat cgacgaggaa gcggaagaga acctgtacga
     4261 aaatggagca attacgcccc accgtcaaga aatgagctgg ctctccacgg cgcgccatca
     4321 tagacagatg tggctctgca actgcgccat gcgatcggag accgaccagt aattatcttt
     4381 aatgcccggt tcgacacccg cattctgaaa cagactgctg ccgcacatag cgatccggct
     4441 gactggctgg aagaactgac agtatattgt gcgatggaac tggcagcagg atattatggg
     4501 gccaccaacc gctatggcac gatttctctg gcctgtgctg ccagcaggcc ggactgacat
     4561 gggaagggct ggcacactca gctgatcgcc gacacacgga tgacgtcagg ggtggtaaac
     4621 gctattactg catatcatca ggaattgctg cagcttatgt actgcgataa ccctgaactc
     4681 aactatgcaa acctgaaatc cctgcaaaaa cagctggatg aaatgtatac ctgcatccgt
     4741 aataatcaga tgatgctccc aaactacgga aaaatgcgcc ggtatggtga accggtttca
     4801 acaacgtttg tagagtcgac aatcaacgaa gtgatcgcca gacgatgacc caaaagcaac
     4861 agatgcagtg gggtagggaa ggtacgcatt atttgttaca gaccagaacc gctgtcctga
     4921 ataattaact gcaggacaaa ttcgccagtt ggtacccggg ttgcacgata gacgctcaga
     4981 gtggaggaaa gttatccgcc atggcagcat aactacttcc ccagtttttt cagctctccc
     5041 ttaccaatat cctacgacga tcacatcgaa aaaaaaacac ggaacatttc accactggat
     5101 acgctaatag atcggatatg agcacatatt gcgcccgcct ttcatgcaga gcataaaatg
     5161 aacaagattc acgctaatta ttacctgcaa gattctaccg cacgtcagat agttcaacgc
     5221 acaatgggga ttattccgta ctcggtaaat gtgatgaacg agcatgggat gatcatcgct
     5281 tccggggaag cctcacgttt acatcaacgc catgaaggtg cggtactcgc attgaccgaa
     5341 aaccgggtcg tcgaaattga ttccgcgacc gccaataagc ttaaaggcgt taagcccgga
     5401 atcaatttac cgattaattt tcgcgaccag ctgattggtg tactgggcat taccggcgag
     5461 cctggtgaag tgaaggcata tgcacagttg gtgaagatgg cttcggaatt aatcattgaa
     5521 caaatggctc tcctcgagca aaaacagtgg gataagcgtt accgcgaaga gctgattaat
     5581 cagctgattt tacgcgatcc gccaccggac tcactgaatg cggtggtctc atggttggga
     5641 attaatctcc agcttccgcg ggtggtggtg attatggaac tgcaacaacc tgatcgcgac
     5701 gcactgcata atctgatgga gtattttgaa tatcgcgccc gcgaccattt ggtcacgttt
     5761 tccgacttta atgatctgat aatcttaaaa cctatctcgc taaaaaacgg cgtctgggat
     5821 aaatcgttgg aattaagcga tctgcagaga cttaaacatt acgcccagtc ctgtggtttt
     5881 tcccgactga ttgtcggcgg ctatttctct ggagaaaacg gcctgcatcg ctcttttcag
     5941 accgcgaaat ccacccagtc catggcgaaa agactgaagt tgaagcatca gttcattttt
     6001 catgacgact atgcgctggc atcattatta gatgggcttt ctggtacctg gcaagcggaa
     6061 gaattatcca aaatctggcg caatctggtt gccaaggaca ccaaaggtat gctgcaaaaa
     6121 acgctgcaga attattttga tcataattgt gatctttcgc aaacagcgac tggcttgcat
     6181 attcatgtca atacattacg ctatcgactg cagagagtgg aagatattac aggaatacaa
     6241 atcaataact taaagcagtt attctggctg tatatcggca tggagttaca acgctgaact
     6301 gtatttccct ccaaattttt cgccgtctga cgtcgtattt ttagccgata cacacaagtc
     6361 taacgcggct gctttctcca tactgccccc tgttattttc atggagaaag cagaagatgg
     6421 tttcaataac agcaactggc gccatcatcg cactcgtggt ggcgatcgtt ttaattttac
     6481 gcaaagtctc gccggcttac ggcatgattg tcggtgcgct ggtaggcggt ctggtcggcg
     6541 gcgccgatct ggtgcaaacc gtgacgctaa tggtcactgg cgcgcaaggc attaccaatg
     6601 ccgtgctgcg cattctcgca gcaggtgtac tggccggcgt gttgatagaa tccggcgcgg
     6661 caaacaccat cgctgaaact gtggtgaaaa aagtggggga aagccgggcg cttttagcct
     6721 tagctattgc gacgatgatt ctgaccgccg tcggagtatt tatcgacgtg gcggttatca
     6781 ctgttgcgcc gattgccctg tctattgccc gtcgtgccgg tttgtcgaaa ctcgccattc
     6841 tgctggcgat gattggcggt ggtaaagcgg gtaatgttat gtcgcctaac cccaatgcta
     6901 tcgccgtttc ggacaccttt catgttcccc tcacctccgt gatgatgacc gggataatcc
     6961 ccggtctgtt cggcctgatc gtggcttatt tcctggcaaa atgtctcagc cagaaaggcc
     7021 tgatggtgat gcccgacgaa atgatttcac acgacaacca cggcaagcag ccgggatttc
     7081 ttgccgctat cagcgcgccg ctcgtcgcca ttgtgctgct ggcgctacga cctgttgcgg
     7141 gtatcgtcat cgatccgctg attgccctgc ccgtcggtgg gttagccggt gcgatcctta
     7201 tgggcaggtt taagcaaagt aacgccttta tgatgtccgg gcttacgcgc atggcgccgg
     7261 tagccatcat gctgctcggt accggtacgc tcgccgggat tatcgccaac tcggaactga
     7321 aagatgtgct cattcatgga ctgacctctt ccggtctgcc ttcttatctt ctggcccctg
     7381 tttccggcgc gatcatgtcc atggcaacag cctccaccac ggcgggaacc gctgttgccg
     7441 ccggggtatt cagccagacg ctgctggggc ttggcgtatc agcccttgct ggttccgcca
     7501 tgattcatgc tggcgcaacc gtacttgatc acctgcctca cggcagcttc ttccacgcca
     7561 cgggcggcag tgtcggcatg ctgatccatg aacggttaaa actgctgcct tatgaaaccc
     7621 ttgtcggttt tgtggtcgcc ctgatttcca cactgatgtt tggcgtcttc ggacttgccg
     7681 ggtaaagaca cgatgaaaat agtcattgca ccagattcat ttaaagaaag tttatcggcc
     7741 atgcaggtgg ccaccgctat tgagcaggga tttcgtgaaa tttatcccga ggccgagtat
     7801 gtcaaattgc cgatggctga cggcggtgag ggtaccgtcg aatcaatggt tgaagcaacc
     7861 gaaggccatt atctgttcca gaatgtcacc ggaccgctgg ggacgccagt acaggcgcgc
     7921 tggggtatgc tgggagatgg gaaaacggcg atcgttgaaa tggcctccgc atccggacta
     7981 catcatgttc ctccagaatg ccgtaaccct atgcttacca ccagttatgg cacaggcgaa
     8041 ctgatccttg ccgcactgga ccacggcgtt gagcgaatta ttctcgggat tggcggtagc
     8101 gccaccaatg acggcggcgc aggtatgcta caggcacttg gtgtggcgat gcttgataca
     8161 aacgggaaat cgcttccttt cggcggcggc gcgctaagca acctggcgct catcgatgcc
     8221 tcaggctgcg acgctcgact gaaaaacgtt tctgtcaccg tcgcctgcga cgttaacaat
     8281 ccactctgtg gcgcaacagg agcgtcagcc atcttcggcc cgcaaaaagg agcaacacca
     8341 gaaatggttg cctccttaga agctgcgctg accaacttcg gtatacgact tcaggacgca
     8401 accggacgaa acgtgatgac gacgcctggc gcaggtgccg ctggcggcat gggcgcggcg
     8461 ctgttaggga ttctgaatgc ggagttaaag ccgggagtgg agattgttat tgacgcgctg
     8521 catctcgata gcctggtcgc ggatgccagc ctggtaatca ccggtgaagg tcgtcttgat
     8581 agccagagca tcagtggtaa aacaccgatt ggtgtcgcac gctgcgctaa gcggtatcag
     8641 aaaccggtga tcgcactggc gggcggctta acgaccgacc accagatagt gcatgagcac
     8701 gggctggacg cggtgttctc cgtgctgacg caaatcgcca ctctgccgga agcgctacag
     8761 gatgcggaat acaacctccg cgtttcggca cgcaatattg ccagcgtctg gaaattatca
     8821 cagcagcaga tgcgctaatc ttcagtcagg ccggcaagca gtagctggcc tggatattct
     8881 ttctcaccag gcccatgcta aaacagcctg cttcagtcat cccacgcaag gcactagcat
     8941 ggcaaaaaaa gaggggagta gtggatccca aaagacggtt aagcggttaa attgaatggt
     9001 caataagggg gctgtttggg aaacgaatct atggtcactc ccgtttttgc aacaccgatt
     9061 ttgacgataa gtgggcttgc ttgaatctat ccggcgtctg aatgggattt tattcccgcg
     9121 cctcgatgag ttccgcgcct gatgaacctc aagaaaatat acggcttcaa tgagcctttc
     9181 cgtttcacag gttcctcaac aggccggtgg gccgttcata tcatcaatac cgtgatgctg
     9241 atactccgta cccaccggaa ccgcacctga tgcggttccg gcttaagggc tgtcaggact
     9301 cgcttttggc tgccatcaac aaaccggaag agatgccgga ttatctcttc cgtcaactcg
     9361 gttttaatca gacctggcat gagtggaagc gggacgaaca gcacaggcaa caacaacgcc
     9421 ggcccggaca ctaccggggc atgagtatgt aatgtcaggg acagctatac gccataaaaa
     9481 aatgaaatag atggcctctc tataaagagg ccatgatatt tactaaccct gttcgttaat
     9541 acattctata aagtttttat gtaggctatc tatattttct cgggtatgaa tagctgatat
     9601 tgcacaacgt agaattgcct ttccctcttc cactgtagga tacatagcaa cggttgtagc
     9661 aaaaccacga tcatgcattt ttttgcatat cgttatagct ttttcttcat tgccaattaa
     9721 caatccacgt attggcattg tagtcccttt gtttatgact tcggtttcat taaatttgtc
     9781 atcaaagtat tttataactt gttgtaagtc attttgacgc aaggatactg tgccatcgag
     9841 gtgtaaatct gctgctgcga tacatgcagc aattcctggt aatgatggtg gaccaccaaa
     9901 agcataggta gttccatacg tttttataaa gtttatggtg tcaggataat tgcatgcaat
     9961 aaatccacca tgagagccaa acgctttaga taatgaacca gcgataataa ttctgtcatc
    10021 aagtttgtta tccagacaag acattacata tccggagcca ttttccccat atattgacat
    10081 gccatgagca tcatcaatgt aagcataccc atcaaatctg ttagccattc taacaataaa
    10141 ttcgacagga gccaccccac ccatcgaacc gacactatca gatatagata ttggtaccat
    10201 attatttatt gaagcatatt taaacgcttc ttctatatca tttttatgtt gaaagtcaac
    10261 cctgattgtt tcaccgaact gttgtaatag tcctctattt atttgcaatg ttgcatgcgc
    10321 tgttttctct aaaataaaat atggtttttt attaaacttg taattaggta gtgttcctga
    10381 tgcaagaagt gggagtacag ctaaatggca tggagtaacc gcattaaatg ttaatgtgtg
    10441 cgccccattc attattttat ttaatctctt ttccaattgc tcgaataaat ctacttttac
    10501 gcgcgttctg gcgaccgaaa gttggactcc aaatctttca attgcattag cgattgcatg
    10561 ttttagattt tcatgtgttt ccagtccaag ataagagcat gacacaaact caatcatctc
    10621 cctcccatca ttaacagtga ttaccttacc atttcttctg gtgatcacta gttcatttat
    10681 atgttcgtta aatactcttg tttggtattt gattgaacgt tctatctttt tcttttgcca
    10741 attcattttt tattattccc tataataata gttacgaaca ttgacaataa tgcaagaata
    10801 agacaactaa tacttaaagt ggttcccaaa aaaactccat tatcgcttat ttttactgaa
    10861 aggaaactga ttgtccctgc gcttactgtt tgagtaaagc ctgtaattga tgctgctaaa
    10921 ccagttcgtg aaccgtttat ctgcaatgag atgaaattag ctattggata gtatgcgcca
    10981 agagttaaat tgaaaatcat tgctccaata acacatatta
//
LOCUS       DAZGAN020000146        10792 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGAN020000146 DAZGAN020000000
VERSION     DAZGAN020000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.146"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            185..1327
                     /gene="yghO"
                     /locus_tag="ACHKYO_RS21885"
                     /old_locus_tag="pgaptmp_004377"
     CDS             185..1327
                     /gene="yghO"
                     /locus_tag="ACHKYO_RS21885"
                     /old_locus_tag="pgaptmp_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HGU4431111.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1334..2098)
                     /gene="glcC"
                     /locus_tag="ACHKYO_RS21890"
                     /old_locus_tag="pgaptmp_004378"
     CDS             complement(1334..2098)
                     /gene="glcC"
                     /locus_tag="ACHKYO_RS21890"
                     /old_locus_tag="pgaptmp_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HGU4431112.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2349..3848
                     /gene="glcD"
                     /locus_tag="ACHKYO_RS21895"
                     /old_locus_tag="pgaptmp_004379"
     CDS             2349..3848
                     /gene="glcD"
                     /locus_tag="ACHKYO_RS21895"
                     /old_locus_tag="pgaptmp_004379"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HGU4431113.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3848..4900
                     /gene="glcE"
                     /locus_tag="ACHKYO_RS21900"
                     /old_locus_tag="pgaptmp_004380"
     CDS             3848..4900
                     /gene="glcE"
                     /locus_tag="ACHKYO_RS21900"
                     /old_locus_tag="pgaptmp_004380"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HGU4431114.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4911..6134
                     /gene="glcF"
                     /locus_tag="ACHKYO_RS21905"
                     /old_locus_tag="pgaptmp_004381"
     CDS             4911..6134
                     /gene="glcF"
                     /locus_tag="ACHKYO_RS21905"
                     /old_locus_tag="pgaptmp_004381"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HGU4431115.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6139..6543
                     /locus_tag="ACHKYO_RS21910"
                     /old_locus_tag="pgaptmp_004382"
     CDS             6139..6543
                     /locus_tag="ACHKYO_RS21910"
                     /old_locus_tag="pgaptmp_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HGU4431116.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6565..8736
                     /gene="glcB"
                     /locus_tag="ACHKYO_RS21915"
                     /old_locus_tag="pgaptmp_004383"
     CDS             6565..8736
                     /gene="glcB"
                     /locus_tag="ACHKYO_RS21915"
                     /old_locus_tag="pgaptmp_004383"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HGU4431117.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGQQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9092..10774
                     /gene="glcA"
                     /locus_tag="ACHKYO_RS21920"
                     /old_locus_tag="pgaptmp_004384"
     CDS             9092..10774
                     /gene="glcA"
                     /locus_tag="ACHKYO_RS21920"
                     /old_locus_tag="pgaptmp_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HGU4431118.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2502 a         2725 c         3040 g         2525 t
ORIGIN      
        1 cttgtccggt cgccttaaat cgacaaaagg cattgaggcg cttctgtgta gtctctaacc
       61 ttatcataat cattaataaa tactatttat tctttgtttg tattcattgg gttgattatt
      121 aaggtgtcgt tcgttgctgt tgcaacctgt ttttcaccgt taattatttg ttatccacag
      181 agatatggaa tgcgatctgc tgatgattaa aattgaaaaa gtaattaata aaaatgacct
      241 taaggcattt atcgcttttc cgtcatcact ttatcccgac gatccaaact ggataccccc
      301 tttattcata gaacgcaacg agcatttgtc tgcgaaaaat ccggggacgg accatatcat
      361 ctggcaggcg tgggtggcaa aaaaagcggg gcagatagtt gggcgtatta ccgcgcaaat
      421 agataccttg caccgtgagc gttacggtga agataccggt catttcggca tgattgacgc
      481 cattgatgat ccgcaggttt ttgccgcgtt gtttggcgca gcggaagcgt ggttgaagtc
      541 acaaggtgca agtaagatca gcggtccttt cagcctgaat atcaatcagg aaagcggatt
      601 actgattgaa ggttttgaca caccaccctg tgcgatgatg ccacacggca aaccgtggta
      661 tgccgcgcat attgaacaac tgggttatca caaaggcatt gatttactgg cgtggtggat
      721 gcagcgaacc gatctcactt tctctccggc gctaaaaaaa ctgatggatc aggtgcgcaa
      781 aaaggtgacc attcgctgca tcaatcgtca gcggtttgcc gaagaaatgc agatcctgcg
      841 tgagattttc aactctggct ggcaacacaa ctggggattt gtgccgttta ccgaacatga
      901 attcgcgacc atgggcgatc aacttaaata tctggtgccg gacgatatga tctatatcgc
      961 tgagattgat tctgcaccct gcgcgtttat tgtcggcttg ccgaacatca acgaggcgat
     1021 tgccgatctg aacggatcgc tctttccctt cggctgggca aaattgctgt ggcgtttgaa
     1081 agtcagcggt gtgcgaactg cgcgagtacc gctgatgggc gtgcgcgacg agtatcagtt
     1141 cagccgcatc ggcccggtga ttgcgctgtt attgattgaa gccttacgcg atccgtttgc
     1201 ccgccggaag atcgatgcgc tggagatgtc atggatcctc gaaaccaata caggcatgaa
     1261 taacatgctg gaacgcattg gggcggagcc gtacaagcgt taccgattgt atgaaaagca
     1321 gatttaatac cagctaactc aggttcatct ccagcggcac cgccgagcga atcaaatggt
     1381 ggccttccag ctcgatatcg tggagattct ttttcacggt ccgcacatga tcgcgtgctg
     1441 cgcgctgggc gacgtgcggc agccgctgca acaccgcgtt gtagatcctc gcatgctggc
     1501 gatcgatctg ctttttttgc tgtggtcgat ggtagagatt atttacgctg gcaaacactg
     1561 aattaaacat cagatcggtc aatgattgca gcgtaaacac cagcacctga ttgtgagaag
     1621 cctgacaaat ggcgagatgg aaagcgtgat ccaactgcgc atgttcgatc agcgaaatct
     1681 ctttgttgtt ctcactggcg gcgagcattt tttcataaca gcgggttatc acaacaaaat
     1741 cagcctgcgt tcccagcgtt gccgccagcc ttgccgattc gccctccagt aatgcgcgaa
     1801 cgtcgagcag atcgtacagc gttcgcggct gcgtactgaa cagatggatc agcgggctgg
     1861 tgtcctgcac ccgattaagc cgtgcgacac gagaatcgcg accctgcgcc gtttcaataa
     1921 tcccgcgccc gcgcagcacg gtcagccctt cacgcagtgc ggagcgtgag aagccgagct
     1981 tttcacacag tcgacgttcc gagggaagtg gctgaccgac cttcagtacg ccgtcgataa
     2041 ttaaccgttc gatactctct gcaaccactt cgcaaatagg gcgacgttca tctttcattc
     2101 ccggacctcg tgcacaggta ggaccaattt ttctgttctt aactcgcaaa acacgcacat
     2161 cacgtaagtg attatgttac atcaatttaa cattgagtta accaaggcaa ggtcacagag
     2221 ctggaaaaaa aatggtctga ccggtagcta aagagataga cgaaaacgaa aagcccgctt
     2281 aataactgtt cacagaagca gcgcgcaaaa atcagctgcc acacaacaca acaaagcgaa
     2341 gcctactcat gagcatcttg tacgaagagc gtcttgatgg cgctttaccc gatgtcgacc
     2401 gcacatcggt actgatggca ctgcgtgagc atgtccctgg acttgagatc ctgcataccg
     2461 atgaggagat cattccttac gagtgtgacg ggttgagcgc gtatcgcacg cgtccattac
     2521 tggttgttct gcctaagcaa atggaacagg tgacagcgat tcttgctgtc tgccatcgcc
     2581 tgcgtgtacc ggtggtgacc cgtggtgcag gcaccgggct ttctggtggc gcgctgccgc
     2641 tggaaaaagg tgtgttgttg gtgatggcgc gctttaaaga gatcctcgac attaaccccg
     2701 ttggtcgccg cgcgcgcgtg cagccaggcg tgcgtaacct ggcgatctcc caggccgttg
     2761 caccgcataa tctctactac gcaccggacc cttcctcaca aatcgcctgt tccattggcg
     2821 gcaacgtggc agaaaatgcc ggcggcgtcc actgcctgaa atatggtctg accgtacata
     2881 acctgctgaa aattgaagtg caaacgctgg acggcgaggc gctgacgctg ggatcggacg
     2941 cgctggattc acctggtttt gacctgctgg cgctgttcac cggatcggaa ggtatgctcg
     3001 gcgtgaccac cgaagtgacg gtaaaactgc tgccgaagcc gcccgtggcg cgggttctgt
     3061 tagccagctt tgactcggta gaaaaagccg gacttgcagt tggtgacatc atcgccaatg
     3121 gcattatccc cggcgggctg gagatgatgg ataacctgtc gatccgcgcg gcggaagatt
     3181 ttattcatgc cggttatccc gtcgacgccg aagcgatttt gttatgcgag ctggacggcg
     3241 tggagtctga cgtacaggaa gactgcgagc gggttaacga catcttgttg aaagcgggcg
     3301 cgactgacgt ccgtctggca caggacgaag cagagcgcgt acgtttctgg gccggtcgca
     3361 aaaatgcgtt cccggcggta ggacgtatct ccccggatta ctactgcatg gatggcacca
     3421 tcccgcgtcg cgccctgcct ggcgtactgg aaggcattgc ccgtttatcg cagcaatatg
     3481 atttacgtgt tgccaacgtc tttcatgccg gagatggcaa catgcacccg ttaatccttt
     3541 tcgatgccaa cgaacccggt gaatttgccc gcgcggaaga gctgggtggg aagatcctcg
     3601 aactctgcgt tgaagttggc ggcagcatca gtggggaaca tggcatcggg cgagaaaaaa
     3661 tcaatcaaat gtgcgcccag ttcaacagcg atgaaatcac gaccttccat gcggtcaagg
     3721 cggcgtttga ccccgatggt ttgctgaacc ctgggaaaaa cattcccacg ctacaccgct
     3781 gtgctgaatt tggtgccatg catgtgcatc acggtcattt acctttccct gaactggagc
     3841 gtttctgatg ctacgcgagt gtgattacag ccaggcgctg ctggagcagg tgaatcaggc
     3901 aattagcgat aaaacgccgc tggtgattca gggcagcaat agcaaagcct ttttaggtcg
     3961 ccctgtcacc gggcaaacgc tggatgtgcg ttgtcatcgc ggcattgtta attacgaccc
     4021 gaccgagctg gtgataaccg cgcgtgccgg aacgccgctg gtggcgattg aagcggcgct
     4081 ggagagcgcc gggcaaatgc tcccctgcga gccgccgcat tatggtgaag aagccacctg
     4141 gggcgggatg gtcgcctgcg ggctggcggg gccgcgtcgc ccgtggagcg gttcggtccg
     4201 cgattttgtc ctcggcacgc gcatcattac cggcgctgga aaacatctgc gttttggtgg
     4261 cgaagtgatg aaaaacgttg ccggatacga tctctcacgg ttaatggccg gaagctacgg
     4321 ttgtcttggc gtgctcactg aaatctcgat gaaagtgtta ccgcgaccgc gcgcctccct
     4381 gagcctgcgt cgggaaatca gcctgcaaga agccattaac gaaatcgccc agtggcaact
     4441 ccagccgcta cccattagtg gcttatgtta cttcgacaat gcgttgtgga tccgccttga
     4501 gggcggcgaa ggatcggtaa aagcggcgcg tgaactgctg gggggcgaag aggttgcggg
     4561 tcagttctgg cagcaattgc gtgaacaaca actgccgttc ttctcgttac caggtacctt
     4621 atggcgcatt tcattaccca gcgatgcgcc gatgatggat ttacccggcg agcaactgat
     4681 cgactggggc ggagcgttac gctggctgaa atcgacagcc gaggacaatc aaatccatcg
     4741 catcgcccgc aacgctggcg gtcatgcgac ccgctttagt gccggagatg gtggctttgc
     4801 cccgctatcg gctcctttat tccgctatca ccagcagctt aaacagcagc tcgacccttg
     4861 cggcgtgttt aaccccggtc gcatgtacgc ggaactttga ggagcaggct atgcaaaccc
     4921 aattaactga agagatgcgg cagaacgcgc gcgcgctgga agccgacagc atcctgcgcg
     4981 cctgtgttca ctgcggattt tgtaccgcaa cctgcccaac ctatcagctt ctgggcgatg
     5041 aactggacgg gccgcgcggg cgcatctatc tgattaaaca ggtgctggaa ggcaacgaag
     5101 tcacgcttaa aacacaggag catctcgatc gctgcctcac ttgccgcaat tgtgaaacca
     5161 cctgtccttc tggtgtgcgc tatcacaatt tgctggatat cgggcgtgat attgtcgagc
     5221 agaaagtgaa acgcccactg ccggagcgaa tgctgcgcga aggattgcgc caggtagtgc
     5281 cgcgtccggc ggtcttccgt gcgctgacgc aggtagggct ggtgctgcga ccgtttttac
     5341 cggaacaggt cagagcaaaa ctgcctgctg aaacggtgaa agctaaaccg cgtccgccgc
     5401 tgcgccataa gcgtcgggtt ttaatgctgg aaggctgcgc ccagcctacg ctttcgccca
     5461 acaccaacgc ggcaactgcg cgagtgctgg atcgtctggg gatcagcgtc atgtcagcta
     5521 acgaagcagg ctgttgtggc gcggtggact atcatcttaa tgcgcaggag aaagggctgg
     5581 cacgggcgcg caataatatt gatgcctggt ggcccgcgat tgaagcaggt gccgaggcaa
     5641 ttttgcaaac cgccagcggt tgcggcgcgt ttgtcaaaga gtacgggcag atgctgaaaa
     5701 acgatgcgtt atatgccgat aaagcgcgtc aagtcagtga actggcggtc gatttagtcg
     5761 agcttctgcg cgaggaaccg ctggaaaaac tggcagttcg cggcgataaa aaactggcct
     5821 tccactgccc gtgtacccta caacatgcgc aaaagctgaa cggcgaagtg gaaaaagtgt
     5881 tgcttcgtct tggatttacc ttaacggacg ttcccgacag ccatctgtgc tgcggttcag
     5941 cgggaacata tgcgttaacg catcccgatc tggcacgcca gctgcgggat aacaaaatga
     6001 atgcgctgga aagcggcaaa ccggaaatga tcgtcaccgc caacattggt tgccagacgc
     6061 atctggcgag cgccggtcgt acctctgtgc gtcactggat tgaaattgta gaacaagccc
     6121 ttgaaaagga ataaaaaaat gaaaactaaa gtcattctta gccagcaaat ggcgagtgca
     6181 attattgccg caggtcagga agaggcgcag aaaaataact ggtctgtttc cattgctgtt
     6241 gccgatgacg gcggtcatct gctggcgtta agtcgcatgg acgattgcgc gccgattgcg
     6301 gcttacatct cccaggagaa agcgcgtacc gccgcgctgg ggcgtcgtga aactaagggc
     6361 tatgaagaga tggtgaacaa cggacgtacc gcgttcgtga ctgcgccgtt actgacgtcg
     6421 ctggaaggtg gcgtaccggt tgttgttgat gggcaaatta ttggtgccgt gggcgtttct
     6481 ggtttaaccg gagcacagga tgcgcaggtc gcgaaagcgg cagcagcggt gttggcgaaa
     6541 taagcgaaaa cgaggagata aacaatgagt caaaccataa cccagggccg tttacgcatt
     6601 gacgccaatt ttaaacgttt tgtggatgaa gaagttttac cgggaacagg gctggacgct
     6661 gcggcgttct ggcgcaattt tgatgaaatc gttcacgatc tggcaccaga aaatcgtcag
     6721 ttgctggcag aacgcgatcg cattcaggcg gcgcttgatg agtggcatcg cagcaatccg
     6781 gggccggtaa aagataaagc ggcctataaa tctttcctgc gtgaactggg ctacctggtg
     6841 ccgcaaccgg agcgcgtgac ggtggaaacc acgggcattg acagcgaaat caccagtcag
     6901 gcggggccac agctggtggt tccggcaatg aacgcccgct acgcgctgaa cgcggcgaac
     6961 gctcgctggg gctcactgta cgatgcgtta tacggcagcg acatcatccc gcaggaaggg
     7021 gcgatggtca gcggctacga tccgcaacgc ggtgagcagg ttatcgcctg ggttcggcgt
     7081 ttcctcgatg aatctctacc gctggaaaac ggcagctatc aggatgtggt ggcgtttaag
     7141 gtggtcgata aacaattacg catccagttg aaaaatggta aagaaaccac gttacgtacc
     7201 ccggcgcagt ttgtcggtta ccgtggcgat gccgctgcgc cgacctgcat tttgctgaaa
     7261 aataacggcc tgcatattga actgcaaatt gatgccaacg ggcggattgg caaagacgat
     7321 ccggcgcaca tcaacgatgt tatcgtcgaa gcggccatca gtaccattct cgactgcgaa
     7381 gattcggtcg cggcggttga tgcggaagat aaaatcctgc tgtaccgcaa cctgctgggc
     7441 ctgatgcagg ggactctgca agagaaaatg gagaaaaacg gtcagcaaat cgtgcgtaaa
     7501 ctgaatgacg atcgtcatta caccgccgcc gatggctctg aaatttctct gcacggacgc
     7561 tcgctgctgt ttatccgcaa cgtgggtcat ttgatgacca ttcctgtgat ttgggacagc
     7621 gaaggcaatg aaatcccgga aggcattctt gatggcgtca tgactggcgc gattgccctc
     7681 tatgatttaa aagtgcagaa aaactcgcgc actggcagcg tctatattgt gaaaccgaaa
     7741 atgcacggtc cgcaggaagt ggcgttcgcc aacaaactgt ttacccgcat tgagacaatg
     7801 ctcggtatgg caccgaatac cctgaaaatg ggcattatgg atgaagaacg tcggacctcg
     7861 ctgaacttgc gtagctgtat cgctcaggcg cgcaaccgcg tggcgttcat caataccggt
     7921 ttcctcgacc gtaccggcga tgaaatgcat tcggtgatgg aagctggccc gatgctgcgt
     7981 aaaaaccaga tgaaatcgac gccgtggatc aaagcctacg agcgtaataa cgtgctttcc
     8041 ggtctgttct gtgggctgcg cggtaaagcg caaattggta aaggcatgtg ggcaatgccg
     8101 gacctgatgg cagacatgta cagccagaag ggcgaccaac tgcgtgccgg ggcaaacaca
     8161 gcctgggttc cgtcaccaac cgctgctacg ctccatgcgc tgcactacca ccaaaccaac
     8221 gtacagagcg tgcaagccaa cattgcccag accgagttca atgctgaatt tgaaccgctg
     8281 ctggacgatc tgctgactat tccggtggct gaaaacgcta actggtcggt ggaagagatc
     8341 caacaagagc tggataacaa cgtgcagggg attctggggt acgtggtgcg ctgggtggag
     8401 caggggattg gttgttcaaa agtgccggat attcacaatg tggcgctgat ggaagaccgc
     8461 gcaacgctgc gtatctccag ccagcatatc gccaactggt tacgtcacgg tattctgacc
     8521 aaagaacagg tgcaggcgtc gctggagaat atggcgaaag tggttgatca gcaaaacgct
     8581 ggcgatccgg cttatcgtcc gatggcgggg aatttcgcta actcgtgtgc ttttaaagct
     8641 gccagcgatt taatcttcct cggcgtgaaa cagccaaacg gctataccga accgttatta
     8701 cacgcctggc gtttacgcga aaaagaaagt cattaatagc gtcaaaaaaa gccccggcaa
     8761 gttttattgt cggggctatg atagtaactg cggtagtaca tttatcccgg aaataaccgg
     8821 agcgattatc agggtttaac aatattaaat acccgtagcg cattactagc gggcctataa
     8881 caacaatatg gacatcttaa cgatactctg cgcctgcgtg tgcagaacag aaacccttac
     8941 ccgatatact tgttatgcca gaggtagcaa ttgcgttagc ggcgtactgt tgctgtaaag
     9001 tataacgcct atattttaca gttcagtcct cttttgctta ttacaacctt aagtatatca
     9061 agcatataaa gataataaga gactgaacaa tatggttacc tggacccaaa tgtatatgcc
     9121 gatgggagga ctggggctat ccgctctggt cgccctgatc ccgataatat tcttcttcgt
     9181 tgcactcgcg gtattacgtc tgaaaggaca tgtcgctgga gcaataaccc ttatattatc
     9241 tatcctgatt gcaatattcg cctttaaaat gccgattgat atggcttttg ctgctgcggg
     9301 ctatggcttt atttatggat tatggcctat tgcgtggatt attgtcgcgg cggtgttcct
     9361 ctataaatta accgttgcca gcggtcagtt cgatattatc cgcagctcgg ttatctccat
     9421 caccgacgat cagcgtttgc aggtgttact gattggtttc tcctttggtg cgttactgga
     9481 aggagcggct ggctttggtg cgccggtggc gattaccggt gcgctgctgg tgggcctggg
     9541 cttcaaaccg ttatacgcgg cggggctgtg tctgattgcc aacactgcgc cagtggcgtt
     9601 tggtgcgttg ggcgtgccga ttctggtcgc cggtcaggta acgggaatcg atccgttcca
     9661 cattggcgca atggcgggac gtcagttacc gttcctgtcg gttcttgtgc cgttctggct
     9721 ggtagcaatg atggacggct ggaaaggggt gaaagagacg tggccagcgg cgctggttgc
     9781 tgggggaagc tttgctgtca ctcagttctt tacctctaac tatattggtc cggaactgcc
     9841 ggatattact tcggcgctgg tgagtatcgt ctcactcgct ttattcctta aagtctggcg
     9901 gccgaaaaat accgaaacgg caatcagcat ggggcaatcc gcaggtgcga tggtggtaaa
     9961 taagccatct tctggcggtc ccgtgccttc agaatatagt ctggggcaaa tcattcgtgc
    10021 gtggtcaccg tttttaatct taacggtgct ggtcaccatc tggaccatga agccgtttaa
    10081 agcgttattt gctccgggcg gcgcgtttta ttcactggtg attaatttcc agatccctca
    10141 tttgcatcaa caagtgttga aagcggcacc cattgtcgcc caaccaacgc caatggatgc
    10201 ggtgtttaaa ttcgaccctc tctcggctgg cggcaccgct atttttattg cggcgattat
    10261 ctctatcttc atcctcggcg tgggcatcaa gaaagggatt ggcgtctttg ccgaaacgct
    10321 aattagtctg aagtggccga tattgtcgat tggcatggtg ctggcgttcg ccttcgtcac
    10381 caactattcc ggcatgtcca ccacgttggc gctggtactg gcaggtacag gcgtgatgtt
    10441 cccgtttttc tcaccgttcc tcggctggct gggggtattc cttaccggct cggacacttc
    10501 ttcaaacgcc ctgtttggtt cactgcaatc gaccacggcg cagcaaatca acgtctctga
    10561 cactctgctg gtggcagcaa ataccagcgg cggcgtaact ggcaagatga tctccccgca
    10621 atctatcgcc gtggcctgcg ccgcgacggg catggtgggc cgagaatctg aactattccg
    10681 ctacaccgtg aagcacagtc tgatttttgc cagcgttatc ggcgttatca cgttgttgca
    10741 ggcgtatgtg tttactggaa tgttggtctc gtaaatcaat aatgccggat ga
//
LOCUS       DAZGAN020000147        10708 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGAN020000147 DAZGAN020000000
VERSION     DAZGAN020000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.147"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..5958
                     /locus_tag="ACHKYO_RS21925"
                     /old_locus_tag="pgaptmp_004385"
     CDS             <1..5958
                     /locus_tag="ACHKYO_RS21925"
                     /old_locus_tag="pgaptmp_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HGU4431119.1"
                     /translation="AVSIDNVKPGVTTDWKETADGVYKATYTAYTRGSGLTAKLLMQN
                     WNEDLHTAGFIIDANPQSAKIATLSASNNGVLANENAANTVSVNVADEGSNPINDHTV
                     TFAVLSGSATSFNNQNTAKTDVNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVG
                     DSSTAQVELQKSKNEVVADGNDSATMTATVRDAKGNLLNDVKVTFNVNSAEAKLSQTE
                     VNSHDGIATATLTSLKNGDYRVTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITV
                     TNTAPLHMTATLQDKNGNPLKDKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLT
                     GTLAGTHMITARLANSNVSDTQPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATV
                     KDPFDNVVKNLSVVFRTSPADTQLSLKALNTNENGIAEVTLKGTVLGVHTAEAILLNG
                     KSDTKIVNIVPDTSNAQVTLNIPAQQVVTNNSDSVQLTAMVKDPSNHPVAGITVNFTM
                     PQDVAANFTLENNGIAITQANGEAHVTLKGKKAGMHTVTATLGNNNASDAQPVTFVAD
                     KDSAVVVLKTSKAEIIGNGVDETTLTATVKDPFDNVVKDLPVTFSTNPADTQLSQSTS
                     NTNDSGVAEVTLKDTVLGVHTVEATLLNGNGYTTTVNIAPDASNAQVTLNIPAQQVVT
                     NNSDSVQLTAMVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLK
                     GKKAGTHTVTATLGNNNTSDSQPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATV
                     KDQFDNEVNNLPVTFSSASSGLTLTPGVSNTNESGIAQATLAGVAFGEQTVTASLANN
                     GASDNKTVHFIGDTAAAKIIELTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVT
                     VNFTSRTNSAEMTNGGQAVTNEQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLS
                     TSINVNADASTAHLTLLQALFDTVSAGDTTNLYIEVKDNYGNGVPQQEVTLRVSPSEG
                     VTPSNNAIYTTNHDGNFYTSFTATKAGVYQVTATLENGDSMQQTVTYVLNVANAEITL
                     AASKDPVIADNNDLTTLTATVADTEGNAIANTEVTFTLPEDVRANFTLSDGGKAITDT
                     EGKAKVTLKGIKAGAHTVTASMAGSKSGQLVVNFTADTLTAQVNLNVTEDNFIANNIG
                     MTKLQATVTDGNGNPFANEAVTFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKS
                     GTYPVTVSVINYGVSDTKQVTLIADAGTATLASLTSVYSFVVSTTEGATMTASVTDAN
                     GNPVEGIKVNFRGTSVTLSSTSVETDDQGFAEILVTSTEVGLKTVSASLADKPTEVIS
                     RLLNAKADINSATITSLEIPEGQLMVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEH
                     MTISQNIVSTDTHGIAEVSMTPERNGSYMVKASLANGASLEKQLEAIDEKLTLTASSP
                     LIGVYAPTGTTLTATLTSANGTPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLT
                     SNKVGTYTVTASFHNGVTIQTQTTVKVTGNSSTAHVASFIADPSTIAATNSDLSTLKA
                     TVEDGSGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTGSVTVSAVTT
                     AGGMQTVDITLVAGPADASQSVLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGM
                     EFVQSGTNVPYMKISAIDYSLNINGDYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQ
                     FTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSAS
                     WVDVDATGKVTFKNVGSNWERITATPKSGGPSYVYEIRVKSWWVNSGDAFMIYSLAEN
                     FCSSNGYTLPRADHLNHSRSRGIGSLYSEWGDMGHYTTDAGFQSNMYWSSSPANSSEQ
                     YVVSLATGDQSVFEKLGFAYATCYKNL"
     gene            complement(6220..7272)
                     /locus_tag="ACHKYO_RS21930"
                     /old_locus_tag="pgaptmp_004386"
     CDS             complement(6220..7272)
                     /locus_tag="ACHKYO_RS21930"
                     /old_locus_tag="pgaptmp_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HGU4431120.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIEKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            7587..8903
                     /gene="shiA"
                     /locus_tag="ACHKYO_RS21935"
                     /old_locus_tag="pgaptmp_004387"
     CDS             7587..8903
                     /gene="shiA"
                     /locus_tag="ACHKYO_RS21935"
                     /old_locus_tag="pgaptmp_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HGU4431121.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            9005..10459
                     /gene="amn"
                     /locus_tag="ACHKYO_RS21940"
                     /old_locus_tag="pgaptmp_004388"
     CDS             9005..10459
                     /gene="amn"
                     /locus_tag="ACHKYO_RS21940"
                     /old_locus_tag="pgaptmp_004388"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HGU4431122.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         2926 a         2572 c         2529 g         2681 t
ORIGIN      
        1 gcagtcagca tcgacaacgt gaaaccagga gtcactacag actggaaaga aaccgcagat
       61 ggcgtctata aggcgaccta taccgcctat accagaggca gtgggcttac tgcgaagcta
      121 ttaatgcaaa actggaatga agatttacat accgctggat ttatcatcga cgccaacccg
      181 cagtcagcga aaattgcgac attatctgcc agcaataatg gtgtgctcgc caatgagaat
      241 gcagcaaaca ccgtctcggt caatgtcgct gatgaaggaa gcaacccaat caatgatcat
      301 accgtcacgt ttgcggtatt aagcggatcg gcaacttcct tcaacaatca aaacaccgca
      361 aaaacggatg ttaatggtct ggcgactttt gatctgaaaa gtagtaagca ggaagacaac
      421 acggttgaag ttacccttga aaatggcgtg aaacaaacgt taatcgtcag ttttgtcggc
      481 gactcgagta ccgcgcaggt tgagctgcag aagtcgaaaa atgaagtggt cgctgacggc
      541 aatgatagcg ccacaatgac cgcgacagtc cgggatgcaa aaggcaacct gctcaatgac
      601 gtcaaggtca ctttcaatgt taattcagca gaggcgaaac tgagccaaac agaagtgaat
      661 agccacgacg ggatcgccac agctacgctg accagtttga aaaatggtga ttatagggtt
      721 acggcctctg tgagctctgg ttcccaggct aatcaacagg tgaattttat cggtgatcaa
      781 agtactgctg ccctgaccct cagtgtgcct tcaggtgata tcaccgtcac caacacagct
      841 ccgctacata tgactgcaac cttgcaggat aaaaatggca acccattaaa agacaaagaa
      901 atcaccttct ctgtgccaaa cgacgtcgca agtcggttct cgattagcaa cagcggaaaa
      961 ggcatgacgg atagcaacgg gactgcaatc gcctccctga ccggcacgtt agcgggcacg
     1021 catatgatca cggctcgtct ggctaacagc aatgtcagcg atacacagcc aatgacgttt
     1081 gtggcggata aagacagagc ggttgtcgtt ctgcaaacat cgaaagcgga aatcattggg
     1141 aatggcgtgg atgagacaac tctgacagca acagtgaaag atccttttga taacgtggtt
     1201 aaaaatctgt cagtagtctt ccgcacctcc cccgcagaca cgcaactgag tctgaaggcg
     1261 cttaatacta atgagaacgg tattgctgaa gttaccctta aaggcacggt tttgggtgtt
     1321 catacagccg aagccatact gcttaacggt aaaagcgata cgaaaatcgt caatattgtg
     1381 cccgatacca gcaacgcgca ggtcaccctg aacatcccgg ctcaacaggt ggtgacgaat
     1441 aacagcgaca gcgtacagct gacggcgatg gtgaaagatc cgtcgaatca tccggtggcg
     1501 ggaataacgg tgaacttcac catgccacag gacgttgcgg caaactttac ccttgaaaat
     1561 aacggtattg ccatcactca ggccaatggc gaagcgcatg tcacgctcaa aggtaaaaaa
     1621 gcgggcatgc atacggttac cgcaacgttg ggtaacaata atgccagcga tgcgcaacca
     1681 gtcacattcg tggcggataa ggacagcgca gttgtcgttc tgaaaacatc gaaagcggaa
     1741 atcattggga atggcgtgga tgagacgact ctgacggcaa cagtgaaaga tccttttgat
     1801 aacgtagtaa aagatctacc ggtcaccttc agtaccaacc cggcagatac tcaacttagt
     1861 cagagcacaa gcaatactaa cgacagtggt gttgctgaag ttacccttaa ggacacggtt
     1921 ttgggtgttc atacagtcga agccacgctg cttaacggca atggctatac gacgacagtc
     1981 aatattgcgc ccgatgccag caacgcgcag gtcaccctga acatccctgc tcaacaggtg
     2041 gtgacgaata acagtgacag cgtgcagctt acggcgatgg tgaaagatcc gtcgaatcat
     2101 ccggtggcgg ggataacggt caacttcacc atgccacagg acgttgcggc aaactttacc
     2161 ctcgaaaata acggcattgc catcacccaa gccaatgggg aagcgcatgt cacgctgaaa
     2221 ggtaaaaaag cgggtacgca tacggttacc gcaacgctgg gtaataacaa taccagtgat
     2281 tcgcagccgg taacgtttgt ggcggacaaa acctcggctc aggttgtcct gcagatgtca
     2341 aaagatgaga tcacaggtaa tggcgtcgat aacgcaacgc taactgcaac ggttaaagat
     2401 cagttcgaca atgaggtgaa taatcttccg gtaacattca gctcagcctc ttcaggactc
     2461 accctgaccc cgggagtaag taataccaat gagtctggca tcgcgcaggc cactctcgca
     2521 ggcgttgcct ttggtgagca gacggtcact gcatcactgg ctaataatgg tgccagcgac
     2581 aacaaaactg tgcattttat tggcgacaca gcggcggcaa aaattatcga gttgacgcct
     2641 gtcccagaca gcataatcgc cggtaccccg cagaacagct ccggcagcgt catcaccgcc
     2701 acagtcgttg ataataatgg ctttccggtg aaaggtgtga ctgtgaactt caccagcaga
     2761 acaaactctg ccgaaatgac gaatggcggc caagccgtaa cgaacgaaca gggtaaggct
     2821 accgtcactt ataccaatac ccgctcctcg atagaatcag gagcgagacc ggataccgtt
     2881 gaggccagtc tggaaaatgg tagctccacg cttagcacat caattaatgt caacgctgat
     2941 gcgtctacgg cacatctcac cttgctacag gcactttttg atacagtctc cgcaggcgac
     3001 actaccaatc tgtatattga ggtgaaggat aattacggca acggtgtacc ccagcaggag
     3061 gtaaccctca gagtatcacc aagtgaaggc gtgaccccca gtaataacgc tatatatact
     3121 accaaccacg acggcaattt ttacacaagc tttaccgcta caaaagccgg ggtttatcaa
     3181 gtgacggcaa ccctcgaaaa tggcgattcg atgcaacaaa cagtgaccta tgtgctgaac
     3241 gtcgcgaatg ccgaaatcac gctggcagcc tcgaaggatc cggtgattgc cgacaataac
     3301 gatctcacga cactaacagc aacagtcgct gatacagagg gcaatgcgat agccaacact
     3361 gaagtaacat ttactctgcc ggaagatgtg agggcgaact tcacgctgag cgatggcggt
     3421 aaagcgatta ctgatactga aggcaaagcg aaagtcacgc tgaaaggtat aaaagcaggc
     3481 gctcatactg ttacagcatc gatggctggc agtaagagtg gacaactggt ggtgaacttt
     3541 actgcggata cgctcactgc gcaggttaat cttaacgtta ccgaggacaa tttcatcgcc
     3601 aataacattg ggatgaccaa gctgcaggca acagtgactg atggaaacgg caacccattc
     3661 gccaatgagg cggtgacatt cacgctaccg gcagatgtga gcgcaagctt cactctcgga
     3721 caaggcggtt ccgccattac tgatatcaac ggcaaggctg aagttacact gagcggtacc
     3781 aaatccggca cctatcccgt gacagttagc gtgatcaatt atggtgtcag tgatacgaaa
     3841 caggtgactt tgattgccga tgctggtacc gcaacactag cctccttaac ctctgtatac
     3901 tcattcgtcg tcagcacgac cgagggcgcg accatgactg caagcgtcac tgacgctaac
     3961 ggcaacccgg tagaaggcat aaaagttaat ttccgcggaa cctccgtcac gctaagcagc
     4021 accagcgttg aaacggatga tcagggtttc gctgaaattc ttgtgacaag caccgaagtc
     4081 ggactgaaaa cagtttcagc ctctctggca gataaaccta ctgaagtcat atcgcgatta
     4141 ctgaatgcaa aagcagatat taattctgca acgattacca gtctggagat acctgaaggt
     4201 cagctaatgg tcgcacaaga cgtagcagtt aaagctcacg tcaacgacca gtttggcaac
     4261 ccgattctta atgaatctgt aacattcagt gcagagccac cagagcacat gaccatcagc
     4321 caaaatattg tctctactga tacgcatggt atagccgagg tctccatgac gcccgaaaga
     4381 aacggttcgt atatggtgaa agcatccctg gcgaatggag cctcacttga gaaacaactg
     4441 gaggctattg atgaaaaact gacactcacg gcgtccagtc cgcttatcgg tgtctatgcc
     4501 cctacaggca ctactctgac ggcaacgcta acctctgcaa atggcactcc agtggagggt
     4561 caggtcatca actttagcgt aacgccagaa ggggcgacgt taagtggcgg aaaagtgaga
     4621 actaactctt caggtcaggc tccggtcgtt ctgaccagca ataaagtcgg tacatatacg
     4681 gtgactgcat cgttccataa cggcgtaaca atacagacac agacaaccgt gaaagtcact
     4741 ggcaactcaa gcaccgcaca tgttgctagc tttatcgctg atccatcgac tatcgccgcc
     4801 accaacagtg atttaagtac cttaaaggca acggttgagg atggcagtgg taacctgatc
     4861 gaaggtctca ctgtgtactt cgccttaaaa agcggctctg ccacattaac gtcattaaca
     4921 gcggtgaccg atcaaaacgg aatcgcgaca acaagcgtga aaggagcgat gacaggtagc
     4981 gtcacggtaa gcgcagtcac gaccgctggt ggaatgcaaa cagtagatat aacgctggtg
     5041 gcaggcccgg cagacgcctc gcagtccgtc cttaagaaca atcggtcctc actgaaaggg
     5101 gactttaccg atagtgctga gctacatctt gttttgcacg atatatcagg caatccgatc
     5161 aaagtttctg aagggatgga atttgtgcaa tcaggtacta acgtgcccta tatgaaaatt
     5221 agcgcaattg attacagtct aaatatcaac ggtgattaca aagccactgt tacaggcggc
     5281 ggagagggta tcgcaacgct gatccctgta ttgaatggtg ttcatcaagc tggtctgagt
     5341 accacaatac aattcactcg cgcagaagac aaaataatga gcggtacagt atcagtcaat
     5401 ggtactgacc taccgacaac tacattccct tcgcaggggt tcaccggggc gtattatcag
     5461 ttgaataatg acaactttgc cccaggaaaa acggcggctg attatgagtt ttcaagctct
     5521 gcctcctggg tcgatgttga tgctaccggt aaagtgacat ttaaaaatgt cggcagcaat
     5581 tgggaaagga ttacggcgac gccaaaatca ggaggcccta gctatgtata cgaaatccgt
     5641 gtgaagagtt ggtgggtgaa ctccggcgat gctttcatga tatacagcct tgctgaaaat
     5701 ttttgcagta gcaatggcta cacacttccc cgtgcagatc atttaaacca tagtcgttcc
     5761 cgaggcatcg ggtcactgta cagtgaatgg ggagatatgg ggcattacac gactgacgct
     5821 ggttttcaat caaatatgta ttggtcatct agtcccgcaa actcaagcga acaatacgta
     5881 gtttccctgg caacaggtga tcaaagcgta tttgaaaagc ttgggtttgc ttatgcgaca
     5941 tgttataaaa acctgtgatt ttccgttata atttcttaaa gagtgctcca ttcggagcac
     6001 tcttttttac attccctcta tagagcttgc ccttctctca cagaaaatga ataaataaaa
     6061 ctgctgcacc gcaagaatcg cgttcgatgt gactatgatc gtatccggtg agtacagctt
     6121 tgatccaaag gctggttttc tgtgatgatt gaccagcctc agagtattga tgacaccgcc
     6181 gttcataaca gatcggcatt actatgcata agtactgtct catattctcg ccaaaacact
     6241 ttttaatgcc tttgcgaatt catccagact tatattgtca attttttgcc caacgacgac
     6301 tgactccgaa acatcggcca ttgcgggcca tcgcgttttg taatcagcat aaattgcaac
     6361 aacgggagta ttataggcac ttgccatatg taatattgag gtgtcaggag ttattgcgat
     6421 ataagcatct tttatgattg ctgctgaacg tttaatcgag ggtgataagg ataaacggta
     6481 gacattatta taacaatcca ctaataccct ggctttatct tccccttttg gtccatgaac
     6541 gatcactatt ggtatatctg tttcactttg aattttttct attagatttt ctgcaatcga
     6601 taatgaaaat gtacgttcct gcgagctacc ttctaaatta agcgcaatgt aagatcctaa
     6661 cgatcgcatt tcctcgcgca cctcatcgag tacatcctca cttagtggca attcatatat
     6721 tggcctgact gctggaaaac ccgcctcacg catcataaat gcccatgtca tgggtacagg
     6781 agcccggaga ttctgatcca tccgggacgc gttcttgcac aaaggggagt agcacttcat
     6841 ggttaaacca acaacctgaa aattcgtttt tgctttcaac tgactgataa atatcatcgt
     6901 tttcaggttc tttttacgca tcgcctctat acaaagatcc ggcgtaccgt attgctgtgt
     6961 tatgttcttt gctaaatctt ttatttcttt taatgttgcg tgatcctgca tagtcattgt
     7021 gactaatgtt aatttagtct tttcaagttt aagtgcatta aacacttcta aattaattgt
     7081 cgacgtaaca attaaaagat gcttaatttt atgcaattca agcgcccgaa taacaggaaa
     7141 gatggccata gcatcgccaa tctggtcggg aatatggatg acaacaaagt ctgttttttc
     7201 aatattgaaa ttataagctt tataatcgta gtaactaaat gcaatacgtc tcaacaatga
     7261 tgctaaaaac atacctaacc tcgcctccct actggttata atgcaatgca gtctatcaga
     7321 ctcatcaggg tgccattttg tgcatatgcg gacttttatg tttcatatct ctaacctgtg
     7381 ggtcctctgc ttaatcctta aacaacacca gcaactcctg cgctttcatc ttccatcgaa
     7441 tttttcatgt tgccgctaat cagccataaa atcatttgca gatgcgctct gtcgaggtag
     7501 tctcataagg ttcgtttata gatcgacggc aatgtgagtt accttttcca tactaattat
     7561 aaaaagacag tacaaacagg atcattatgg actccacgct catctccact cgtcccgatg
     7621 aagggacgct ttcgttaagt cgcgcccgac gagctgcgtt aggcagcttc gctggtgccg
     7681 tcgtcgactg gtatgatttt ttactctatg gcatcaccgc cgcactggtg tttaatcgcg
     7741 agtttttccc gcaagtaagc ccggcgatgg gaacgctcgc cgcatttgct acctttggcg
     7801 tcggattcct tttccgtccg ctcggcggtg tcattttcgg tcactttggc gaccgactgg
     7861 gacgtaagcg catgttaatg ctgaccgtct ggatgatggg catcgcgaca gccttgattg
     7921 gtattcttcc ttcattctcg accattgggt ggtgggcacc tattttactg gtgacgctgc
     7981 gtgccattca gggatttgcc gtcggcggcg aatggggagg cgcggcgttg ctttccgttg
     8041 aaagtgcacc aaaaaataaa aaagcctttt acagtagcgg tgtacaagtt ggctacggtg
     8101 taggtttact gctttcaact ggactggttt cattgatcag tatgatgacg actgacgaac
     8161 agtttttaag ctggggctgg cgcattcctt tcctgtttag catcgtactg gtactgggag
     8221 cattgtgggt gcgtaatggc atggaggagt ccgcggaatt tgaacaacag caacataatc
     8281 aagcggccgc gaaaaaacgc attccggtta tcgaagcact attacggcat cctggtgctt
     8341 tcctgaagat tattgcgcta cgactgtgcg agttgctgac gatgtacatc gttaccgcct
     8401 tcgcacttaa ttattcaacc cagaatatgg ggttaccgcg cgaacttttc ctcaatattg
     8461 gtttgctggt aggtggatta agctgcctga caattccctg ttttgcctgg cttgccgatc
     8521 gttttggtcg ccgcagggtt tatatcacag gcgcgttaat cggaacgttg agcgcatttc
     8581 ctttctttat ggcgcttgaa gcacaatcca ttttctggat agttttcttc tccataatgc
     8641 tggcaaacat tgcgcatgac atggtggtgt gtgtgcaaca accgatgttt accgaaatgt
     8701 ttggtgccag ttatcgctat agtggcgctg gagtcggtta tcaggttgcc agtgtggttg
     8761 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
     8821 atagcgtcgc catttatttg ctggctggat gcctgatttc tgcaataacc gctttgttga
     8881 tgaaagacaa tcaacgcgct tgatagtctg gcgaagatca tccgatcttc gccttacact
     8941 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
     9001 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
     9061 gctgtatgag caatctgtag tcgcattacg caacgccatt ggcaactata ttacaagtgg
     9121 cgaattacct gatgaaaacg cccgcaaaca aggtcttttt gtctatccat cactgaccgt
     9181 aacctgggac ggtagcacaa ccaatccccc caaaacgcgc gcatttggtc gttttaccca
     9241 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atcttaatga
     9301 acaacttacg ttgctgtatc aggattatgg tgcgcatatc tctgtgcaac cctcgcagca
     9361 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
     9421 cgctgggtta actcgctact tcccgacaac agaactggcg caaattggag atgaaactgc
     9481 agacggcatt tatcatccaa ctgaattctc cccgctatcg cattttgatg cgcgccgagt
     9541 cgatttttcc ctcgcgcggt tgcgccatta taccggtacg ccagttgaac attttcagcc
     9601 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
     9661 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     9721 caccgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     9781 gatgccagca tggcatttaa ttaccgccga tgctcagggt attactctgg tgaatattgg
     9841 cgtgggaccg tcaaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatgt
     9901 ctggttgatg attggtcact gtggcggatt acgtgaaagt caggccattg gcgattatgt
     9961 acttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
    10021 tcctattccg agcattgctg aagtgcaacg tgcgctttat gacgccacca agctggtgag
    10081 cggcaggccc ggtgaggaag tcaaacagcg gctacgtact ggtactgtgg taaccacaga
    10141 tgacaggaac tgggaattac gttactcagc ttctgcactt cgttttaact taagccgggc
    10201 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
    10261 atacgggaca ctactgtgtg tttcagataa accattacat ggtgagatta aacttcccgg
    10321 ccaggctaac cgtttttatg aaggcgctat ttccgaacat ctgcaaattg gcattcgggc
    10381 gatcgatttg ctgcgcgcag aaggcgaccg actgcattcg cgtaaattac gaacctttaa
    10441 tgagccgccg ttccgataat aagttctgaa ccttcttatc agaaatagat gtgaaggagt
    10501 aagtaagaca cctggcaaat agcctgcaat aatcgtgggc tgtttgcttc cttgggcgga
    10561 tacgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
    10621 cacaattata aaaaatcccg ccaacaatat aagtttttat aaaattaaat ataagattat
    10681 ggctttagaa tatttttatt tctaatag
//
LOCUS       DAZGAN020000148        10613 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGAN020000148 DAZGAN020000000
VERSION     DAZGAN020000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.148"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            72..659
                     /gene="ecpA"
                     /locus_tag="ACHKYO_RS21945"
                     /old_locus_tag="pgaptmp_004389"
                     /gene_synonym="matB"
     CDS             72..659
                     /gene="ecpA"
                     /locus_tag="ACHKYO_RS21945"
                     /old_locus_tag="pgaptmp_004389"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HGU4431123.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            717..1385
                     /gene="ecpB"
                     /locus_tag="ACHKYO_RS21950"
                     /old_locus_tag="pgaptmp_004390"
                     /gene_synonym="yagY"
     CDS             717..1385
                     /gene="ecpB"
                     /locus_tag="ACHKYO_RS21950"
                     /old_locus_tag="pgaptmp_004390"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HGU4431124.1"
                     /translation="MKKHLLPLALLFSGIPPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1411..3936
                     /gene="ecpC"
                     /locus_tag="ACHKYO_RS21955"
                     /old_locus_tag="pgaptmp_004391"
                     /gene_synonym="yagX"
     CDS             1411..3936
                     /gene="ecpC"
                     /locus_tag="ACHKYO_RS21955"
                     /old_locus_tag="pgaptmp_004391"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HGU4431125.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENKS"
     gene            3926..5569
                     /gene="ecpD"
                     /locus_tag="ACHKYO_RS21960"
                     /old_locus_tag="pgaptmp_004392"
     CDS             3926..5569
                     /gene="ecpD"
                     /locus_tag="ACHKYO_RS21960"
                     /old_locus_tag="pgaptmp_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HGU4431126.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQATGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIK"
     gene            5538..6248
                     /gene="ecpE"
                     /locus_tag="ACHKYO_RS21965"
                     /old_locus_tag="pgaptmp_004393"
     CDS             5538..6248
                     /gene="ecpE"
                     /locus_tag="ACHKYO_RS21965"
                     /old_locus_tag="pgaptmp_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HGU4431127.1"
                     /translation="MFRRRGVTLNKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6561..6890
                     /gene="ykgJ"
                     /locus_tag="ACHKYO_RS21970"
                     /old_locus_tag="pgaptmp_004394"
     CDS             6561..6890
                     /gene="ykgJ"
                     /locus_tag="ACHKYO_RS21970"
                     /old_locus_tag="pgaptmp_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU4431128.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7138..7752)
                     /gene="yagU"
                     /locus_tag="ACHKYO_RS21975"
                     /old_locus_tag="pgaptmp_004395"
     CDS             complement(7138..7752)
                     /gene="yagU"
                     /locus_tag="ACHKYO_RS21975"
                     /old_locus_tag="pgaptmp_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HGU4431129.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8170..8859
                     /gene="paoA"
                     /locus_tag="ACHKYO_RS21980"
                     /old_locus_tag="pgaptmp_004396"
     CDS             8170..8859
                     /gene="paoA"
                     /locus_tag="ACHKYO_RS21980"
                     /old_locus_tag="pgaptmp_004396"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HGU4431130.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAALKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            8856..9812
                     /gene="paoB"
                     /locus_tag="ACHKYO_RS21985"
                     /old_locus_tag="pgaptmp_004397"
     CDS             8856..9812
                     /gene="paoB"
                     /locus_tag="ACHKYO_RS21985"
                     /old_locus_tag="pgaptmp_004397"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HGU4431131.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGRGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            9809..>10613
                     /gene="paoC"
                     /locus_tag="ACHKYO_RS21990"
                     /old_locus_tag="pgaptmp_004398"
     CDS             9809..>10613
                     /gene="paoC"
                     /locus_tag="ACHKYO_RS21990"
                     /old_locus_tag="pgaptmp_004398"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HGU4431132.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKV"
BASE COUNT         2675 a         2860 c         2773 g         2305 t
ORIGIN      
        1 ccaggcaagt tagttttaaa aaatgactca ctgggacatc acgtcctcaa ttcaactcgg
       61 gaagaaatgc aatgaaaaaa aaggttctgg caatagctct ggtaacggtg tttaccggca
      121 tgggtgtggc gcaggctgct gacgtaacag ctcaggctgt agcgacctgg tcggcaacag
      181 ccaaaaaaga caccaccagt aagctggttg tgacgccact cggtagcctg gcgttccagt
      241 atgccgaagg cattaaaggt tttaactcac agaaaggtct atttgacgtg gctatcgagg
      301 gtgactcaac ggctaccgcc tttaaactga cctcacgtct tatcaccaac acattaaccc
      361 agttggatac ctcaggttcc acactgaatg tgggcgtgga ttataacggc gcggcagtcg
      421 aaaaaactgg cgataccgtg atgatcgata ccgccaacgg cgtactgggc ggcaacctta
      481 gcccgctggc taacggttac aatgccagca atcgtaccac cgcacaggat ggtttcacct
      541 tcaccatcat cagcggcacc accaatggta ccaccgcagt aaccgattac agcactctac
      601 cggaaggcat ctggagcggc gacgttagcg tacagttcga cgcgacctgg accagttaat
      661 ctctctgatg taccagcagg ggtagccccc ctgctttatt cctggacgga attgttatga
      721 aaaagcacct tctgcctctc gctctgctgt tttccggaat acctccggcc caggcgctgg
      781 atgtcggcga tatatcatcg tttatgaaca gtgacagcag cacgctgagc aaaacgatca
      841 aaaacagtac cgacagtggt cgccttatca atatccgtct cgaacggctc tcttcaccgc
      901 ttgacgacgg gcaggttatc tcaatggaca agccggatga gttgctactc actcccgcca
      961 gcttgctgct acccgcccaa gccagcgaag tgatccgctt cttctataag ggacccgcag
     1021 atgaaaaaga gcgctactac cgcattgtct ggtttgatca ggccctcagt gatgcgcagc
     1081 gcgataatgc caaccgcagc gctgtggcca ctgcttccgc ccgcatcggc accattctgg
     1141 tcgtcgcccc tcgccaggcg aactaccact ttcagtacgc caacggctcc ctgacaaata
     1201 caggaaatgc gacgctgcgg atcctcgcct acggcccttg cctgaaagcc gccaacggta
     1261 aggagtgtaa agagaattac tacctgatgc cgggcaagtc gcgtcgtttt acccgtgtgg
     1321 acactgcgga taacaaagga cgggttgcac tttggcaggg tgataagttc attcccgtga
     1381 aatagatagc tgtgcagatg gataacgaca atgcctttac gacggttctc cccaggactg
     1441 aaagcccagt ttgccttcgg catggtcttt ttgttcgttc aacccgatgc cagcgctgct
     1501 gacataagtg cgcagcaaat aggtggggtg attattccgc aggccttcag tcaggcgctt
     1561 caggacggca tgagcgtccc gctctatatt catctcgccg gtagccaggg tcgccaggac
     1621 gatcagcgaa tcggcagcgc ttttatctgg ctggatgatg gacagctacg catccggaaa
     1681 atacagctgg aagagagtga agataacgcc agtgtcagcg aacaaactcg acagcagctg
     1741 atggctctgg ccaacgcccc gttcaatgag gcccttacca tccccctgac tgacaacgcg
     1801 cagctggatc tcagcttgcg ccaactgctg ctgcagctgg tggtcaagcg cgaagcgctg
     1861 ggcaccgtac tacgctcacg tagcgaagac atcgggcagt ccagtgttaa caccctcagc
     1921 agtaatctga gctataactt gggcgtctat aacaaccagt tgcgtaacgg cgggagcaac
     1981 acatccagct atctgtcgct gaataacgtt actgcgctgc gcgaacatca tgtggtgctc
     2041 gacggctcgc tgtacgggat cggtagcggt caacaggaca gtgaattata taaagcgatg
     2101 tatgaacgcg attttgccgg tcaccgattt gccggtggaa tgctcgacac ctggaacttg
     2161 cagtccttag ggccgatgac cgccatttca gcagggaaga tttacggcct ttcctgggga
     2221 aaccaggcca gctccaccat cttcgacagc agccagtcag ccacgccagt gatcgccttt
     2281 ttaccggcgg cgggcgaagt acatctcacc cgtgatgggc ggctactaag cgttcagaac
     2341 ttcactatgg gcaatcatga agtggatacc cggggtctac cgtacgggat ttacgatgtg
     2401 gaagttgagg tgatcgttaa cggtcgcgtg atcagcaaac gcccccagcg ggtcaataag
     2461 ctgtttagcc gggggcgcgg cgtcggtgca ccactggcgt ggcaggtatg gggcggtagc
     2521 tttcatatgg atcgctggtc ggaaaacggg aaaaagacgc gaccagctaa agagagttgg
     2581 ctagcaggtg cctcgacctc cggctcattg agtacgctta gctgggcggc aacgggatat
     2641 ggatacgata atcaggcggt gggtgaaacc cgtctgacgc tgccgcttgg gggggcgatc
     2701 aacgttaacc tgcaaaatat gctggccagt gacagctcat ggagcagcat cggcagcatc
     2761 agcgccactc taccgggagg ctttagttcg ctgtgggtta accaggaaaa aacccgcatt
     2821 ggcaatcaat tgcgacgtag cgatgccgac aaccgtgcaa tcggcggcac actcaacctg
     2881 aactcactgt ggtcgaagct gggcacattc agcatcagct acaatgatga ccgccgttac
     2941 aacagccatt attacacggc agattactat caaaatgtct acagcggtac ctttggttcg
     3001 cttggcctgc gggccggtat tcagcgctat aacaacggcg acagcaacgc caatacaggg
     3061 aaatatatcg ctctcgatct ctcgctacca ctgggcaact ggtttagcgc agggatgacc
     3121 catcaaaacg gctacaccat ggcaaacctg tcagcacgca aacagtttga tgaaggaacc
     3181 attcgcactg ttggtgccaa tctgtcacgt gccatctccg gcgataccgg tgatgacaaa
     3241 accctcagcg gtggggcgta tgcacagttc gacgctcgtt acgccagcgg aacgctgaac
     3301 gtcaatagcg cggcggacgg ctacatcaat accaacttga ccgccaatgg cagcgtcggc
     3361 tggcagggta aaaacatcgc tgccagcggg cggactgatg gcaacgctgg ggtgatattc
     3421 aacaccgggc tggaggacga cggtcagatc agcgccaaaa tcaacgggcg gattttcccg
     3481 cttaacggca agcgtaacta tctcccgctc tctccctatg gaagatatga ggtggagtta
     3541 cagaacagca aaaactcact cgacagctac gatatcgtca gcggccgcaa aagccatctg
     3601 actctctatc caggcaatgt cgctgtcatt gagccagagg tgaagcagat ggttaccgtc
     3661 tccggtcgta tccgtgcgga agacggcaca ctgctggcta acgcacggat taacaaccat
     3721 atcggccgaa cccgaaccga tgaaaacggc gagtttgtca tggacgtgga taagaaatat
     3781 cccactatcg attttcgcta cagtggcaat aaaacctgcg aagtggcact ggaactcaac
     3841 caggcgcgcg gtgccgtctg ggtcggtgat gtggtctgca gcggcctctc atcgtgggcg
     3901 gcggtgacac agacaggaga agagaataag agttaaccta ctaataacga tgataatttt
     3961 cgcgctaatc tggccagtaa ctgagctcag agcggcagtg agcaaaacaa cctgggcgga
     4021 tgcaccggca cgcgagtttg tgtttgtcga aaacaactca gacgacaact ttttcgtcac
     4081 tcctggcggg gcgctggatc cgcgcctgac cggtgccaac cgctggaccg gtttaaaata
     4141 cactggttca ggaaccatct atcagcaaag cctcggctac attgataacg gttacaacac
     4201 cggcctttat accaactgga agtttgatat gtggctggaa aattcaccag tttcatctcc
     4261 tttaactggc ttgcgctgca tcaactggta cgctgggtgt aatatgacca ccagtcttat
     4321 cctgccgcaa accaccgacg ccagtggatt ttatggcgcg acggtgacca gcggcggcgc
     4381 gaagtggatg cacggcatgt tgtcagacgc gttttaccag tatctgcaac aaatgcccgt
     4441 cggcagcagc tttacaatga ccatcaatgc ctgccagacc tctgtgaact atgacgccag
     4501 cagcggcgca cgctgtaagg atcaggccac cggcaactgg tatgttcgca acgtcaccca
     4561 tacgaaagca gcaaatctgc ggttaataaa tacccactcg ctggcggagg tatttatcaa
     4621 cagcgacgga gtaccgactc tgggcgaagg gaacgccgac tgccggacac agaccatcgg
     4681 cagcctttca ggattaagtt gtaagatggt caactatacc ctgcaaacaa acggactcag
     4741 caacacctca atccatatat tcccggcgat cgccaactcg tcgttagcct cggccgtcgg
     4801 ggcgtacgat atgcagttca gtctgaatgg cagttcatgg aaaccggtga gcaataccgc
     4861 ctattactac accttcaacg agatgaagag cgcagactcg atctatgttt tcttctcgag
     4921 caacttcttt aagcagatgg tgaacctcgg gatcagcgat atcaacacca aagatctatt
     4981 caactttcgc tttcagaaca ccacatcacc ggagtctggc tggtatgaat tttctacctc
     5041 caacacgctg attatcaaac cccgtgattt cagcatcagt attatctccg atgaatatac
     5101 tcagacaccg tcgcgggagg gatatgttgg cagcggcgag tcggcactcg atttcggcta
     5161 tatcgtaacc accagcggta aaacagctgc cgacgaagtg ctgatcaagg tgaccggacc
     5221 cgcgcaggtg attggcgggc gctcctattg tgtcttcagc tccgatgacg gtaaggcgaa
     5281 agtaccgttc ccggcgacac tttcctttat tacccgcaac ggagctacaa aaacctacga
     5341 tgccgggtgc gatgatagct ggcgggatat gaccgatgcg ctgtggttga ccacaccgtg
     5401 gactgatatc tctggcgaag tggggcagat ggataagacc acagtcaaat tttcgattcc
     5461 aatggataac gccatttcgc tgcgtacggt agatgataac ggctggtttg gcgaagtcag
     5521 cgcttcagga gagattcatg ttcaggcgac gtggcgtaac attaaataag gccctgctga
     5581 cagcggtctg tatgctggcg gcacctttga cacaggcgat ttcggtcggc aatctgacat
     5641 tttcgctgcc gtccgagact gactttgtca gcaaacgtgt agtgaataac aacaaaagcg
     5701 cacggatata ccgtattgcc atcagtgcta ttgatagccc gggcagcagt gaattgcgca
     5761 cccgaccggt ggatggtgaa ctgcttttcg ccccccgcca actggcgttg caggctggtg
     5821 agagcgagta ttttaaattt tactatcatg gtccacggga taaccgcgag cgctactacc
     5881 gggtctcatt tcgcgaagtc cccactcgta accagacaag acgtagccca accggcggcg
     5941 aggtcagcac ggagccggtg gtggtgatgg ataccattct ggtagtacga ccacgtcagg
     6001 ttcagtttaa atggtccttc gacaaggtga caggaacggt aagtaacact ggcaacacat
     6061 ggtttaagct actgattaag ccaggatgtg attcgaccga agaggaaggc gatgcctggt
     6121 atctacgtcc gggagacgtg gttcatcagc ctgagttacg tcagccgggg aatcattatc
     6181 tggtctataa cgacaaattc attaagatta gcgattcctg tccggctaag cccccttcgg
     6241 cggactaaga cttatccatc ggtcgaggga agaattccac ctcagagctc caaaaatcgt
     6301 ttaaatgatg tggaagcgat cgtcaatgtc acctgtaacg atgggctttc tcatatgcac
     6361 accatagtgt tcaggagaca cggaaaggta tcaggcagcc tgctaatgag tggtttaatc
     6421 acgaattagt acgtaaaatc ggtaacggct ggaaatcatt caatactcac actatcgaaa
     6481 gttccccagc caaccgcggt acgttcttac atacgatgta ccgctgttct ctttacgatt
     6541 tttagctgta ctggtgaatt atgagcaatc tgaatccatg catgacgtgt ggtgcctgtt
     6601 gtgcattttt ccgcgtctct ttttactggg ccgaagccga cgatgctggc ggtactattc
     6661 ccgccaggct cactgaacaa atatctcctt ttcaccgatg catgagcggt accaatcaga
     6721 aaaacccccg atgtattgcc cttgcaggaa ccccgggcaa aaatgcctgc tgcacgatat
     6781 ataaaaatcg atcgtccaca tgcagagaat tcgccatgtc tggtgagaac ggagtcgtca
     6841 atgaggcttg caatcgtgca agggctaaat acgggctgac accgttataa acatacaata
     6901 attaattgca ctgccccgcc agcgataata gcggggcttc gtttttcagg ggtaacaaaa
     6961 cccgatactt cttctatttg ccagcaacaa tgcctctctt ctgtagcgtt cttcggatct
     7021 acatcatcct gaagtagcgc gatatactca ctgactatct gcatcagaat ataaaaagca
     7081 atgtttttaa cctataaaaa tggtgctgta tttgcgccat ttttatcatt caatgcatta
     7141 tctgtttgag cctaaaggga tctcagggtc tggctcatga gtaattctgt ttcgtaaatc
     7201 tctgcgaata atttcaatag accagaacca gactaaatgt ccaaaaattt cagaaacatt
     7261 ctcataccac gggagatcaa acagaggtgg cgtcagtccc atgagaggga atgaaatcat
     7321 atgaacaaaa agttgggcta aagcacctgc cagtaagccc tgccagagtt taatttttgg
     7381 aaatacttca gcgaccacac aataaccgac agcaaacact atcgaaaaga taatgtgcgt
     7441 aacaccaacc cagttaaaga catgcccggc aaaggtataa acagccgcat tgggatctgt
     7501 cagccccaac cagtctcgaa gaaaaatata cggtggattg agaaaattac gcgagcaatc
     7561 aatttggcct gcagccctga ttaatgattc cgggccacac gctgcattaa acatatccac
     7621 cgggctacgt ggcggcaatg gaacttcagc cccccacttc acgaatgcgg aaacaacgcc
     7681 agcaatcagc ccaatgaatg cagcaagacc ataacgtctg cggttcggtg gagtttgttc
     7741 aaatatattc atatctaccc tgcttgtacc attatgttat acacctcttc aggagtattc
     7801 ataaaacaag gcaaatgtaa agaactgtat tgttttgtat aacaagatgg tttcctaatc
     7861 gccaatgaat ataagctcca tcatttctcc ctatttttat attagaagtg acagagattt
     7921 gcagggtgat gcagagctga aaatcacagg tttccttatt ggtttttgca tcgtacaact
     7981 aaagcaataa accagctccc atcatcatca acatcccggc gaacatgaat ttattagccg
     8041 ggctcatagc tgcattcgcg ttttaagaat aatcctcctg ctgtcgccga ctatgcttaa
     8101 catttaaaaa agcatcagca ctctcgcaac gcactcttat tttccccttt agaataccgg
     8161 aggcctggta tgagcaacca aggcgaatac cccgaagata atcgggttgg gaagcacgag
     8221 ccgcacgatt taagtttgac ccgtcgcgat ctgattaaag tgagcgccgc aacagcggcg
     8281 accgccgtgg tttatcctca ttctacgctg gcggcaagcg ttccggcagc tacacccgcg
     8341 ccagagataa tgcccctgac actgaaggtg aacggcaaaa ccgagcagct tgaggtggat
     8401 acccgaacca cgctactgga cgctttgcgt gaaaatctgc atttgatcgg taccaagaaa
     8461 ggttgcgatc acggacagtg cggagcctgt accgtgctgg tcaatggtcg caggcttaat
     8521 gcctgcctga cgcttgcagt catgcatcag ggggccgaga tcaccaccat tgaaggcctg
     8581 ggttcgccag ataatcttca ccccatgcag gcggccttta tcaagcatga tggcttccag
     8641 tgcggctact gcacctccgg gcaaatttgc tcatcagtag cggcgctaaa agagattcag
     8701 gacggcattc ccagtcacgt cacggtcgat ttggtttccg ctccagaaac aactgccgat
     8761 gagatccgtg aacgtatgag cggcaacatc tgtcgctgtg gtgcatacgc taacatcctt
     8821 gccgccattg aagatgctgc gggggagata aaatcatgaa ggcgtttacc tatgaacgag
     8881 tgaacacccc agccgaggcg gcacttagcg ctcagcgcgt acccggcgca aaatttatcg
     8941 cgggcgggac caatctgctg gacctgatga agctggaaat tgaaacgccc acccacctta
     9001 tcgatgtgaa cggcctcggg ctcgataaga ttgaagtgac cgacgcgggt gggctgcgca
     9061 tcggcgcact ggtacggaac accgacctgg cggctcacga gcgcgtgcgt cgtgattacg
     9121 cggtactctc ccgcgccctg ctcgctggcg catctggtca gttacgtaat caggcaacca
     9181 ccgcaggtaa tctgctccag cgcacgcgct gcccctattt ttacgacacc aatcagccct
     9241 gcaataagcg cctgcccggg agaggctgcg cggcgcttga aggctttagc cgtcagcacg
     9301 cggtggtagg cgtaagtgaa gcctgcatag ccacccatcc gagcgatatg gcggtcgcaa
     9361 tgcggttgct ggatgcggtg gtggaaacca tcacgccgga gggaaagact cgcagtatca
     9421 cactggctga tttttatcac ccaccgggga aaacgccgca cattgaaacc gccctgcttc
     9481 ccggtgagct tatcgttgcg gtgacgttac ctccaccgct cggcggaaaa catatctacc
     9541 gtaaggtgcg cgatcgcgcc tcctacgcct ttgccctggt atcggtcgcg gcgattattc
     9601 agcctgacgg cagcgggcgc gtcgcgctgg gcggagtagc acataagccc tggcgcattg
     9661 aggctgcgga tgctcagcta tcccaggggg cgcaggccgt atatgacgcg ctgttcgcca
     9721 gcgcccatcc caccgctgaa aataccttta aactcctgtt ggcgaagcga acgcttgcct
     9781 ccgtactggc tgaagcgagg gcacaggcat gaaatttgat aaacccgcag gggaaaaccc
     9841 gatcgatcag ctgaaggttg tcggtcgtcc ccatgaccgc atcgacggac cgctgaaaac
     9901 taccggcacg gcacgctacg cctacgaatg gcatgaagaa gcccccaacg ccgcctatgg
     9961 ctatatcgtc ggttccgcca ttgccaaagg acgcctcacc gcccttgata cggacgccgc
    10021 gcaaaaagcg ccgggcgtac tggctgtcat taccgccagt aacgccgggg cactcggcaa
    10081 aggcgacaaa aacaccgcca gactgttagg cggccccact attgagcact atcatcaggc
    10141 cattgcgctg gtagtggccg agaccttcga acaggcgcga gcggcggcct cgctggtgca
    10201 ggcacactat cgccgtaata aaggagctta ctccctggcg gacgaaaaac aggccgtcaa
    10261 tcagccgccg gaagacacgc ccgacaaaaa cgtcggtgac tttgacgggg ctttcacctc
    10321 cgctgcggtg aagattgatg ctacctacac gaccccggac cagagccata tggcgatgga
    10381 gccgcatgcc tcgatggccg tctgggatgg aaataagctt accctctgga cctcaaatca
    10441 gatgattgac tggtgccgca ccgatctggc aaaaacgctg aaagttcccg tggagaatgt
    10501 gcgtattatc tccccgtata tcggcggagg gtttggcggc aagctgttcc tgagaagcga
    10561 tgcgctgctg gcggccctcg ccgcccgagc ggtgaaacgt ccggttaaag tga
//
LOCUS       DAZGAN020000149        10543 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGAN020000149 DAZGAN020000000
VERSION     DAZGAN020000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.149"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2504
                     /locus_tag="ACHKYO_RS21995"
                     /old_locus_tag="pgaptmp_004399"
     CDS             <1..2504
                     /locus_tag="ACHKYO_RS21995"
                     /old_locus_tag="pgaptmp_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HGU4431133.1"
                     /translation="FLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACGYVR
                     TDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIHQQD
                     INVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYRYDF
                     LRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVM
                     VLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELV
                     PFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDV
                     SAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDG
                     DACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQSFEN
                     NYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLS
                     DTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTD
                     TINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGT
                     DDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEV
                     NDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPI
                     VGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCS
                     VVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2501..3589
                     /locus_tag="ACHKYO_RS22000"
                     /old_locus_tag="pgaptmp_004400"
     CDS             2501..3589
                     /locus_tag="ACHKYO_RS22000"
                     /old_locus_tag="pgaptmp_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HGU4431134.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3677..4975)
                     /gene="exuT"
                     /locus_tag="ACHKYO_RS22005"
                     /old_locus_tag="pgaptmp_004401"
     CDS             complement(3677..4975)
                     /gene="exuT"
                     /locus_tag="ACHKYO_RS22005"
                     /old_locus_tag="pgaptmp_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HGU4431135.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5458..6870
                     /gene="uxaC"
                     /locus_tag="ACHKYO_RS22010"
                     /old_locus_tag="pgaptmp_004402"
     CDS             5458..6870
                     /gene="uxaC"
                     /locus_tag="ACHKYO_RS22010"
                     /old_locus_tag="pgaptmp_004402"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HGU4431136.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6885..8372
                     /gene="uxaA"
                     /locus_tag="ACHKYO_RS22015"
                     /old_locus_tag="pgaptmp_004403"
     CDS             6885..8372
                     /gene="uxaA"
                     /locus_tag="ACHKYO_RS22015"
                     /old_locus_tag="pgaptmp_004403"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HGU4431137.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGVHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8455..9006
                     /locus_tag="ACHKYO_RS22020"
                     /old_locus_tag="pgaptmp_004404"
     CDS             8455..9006
                     /locus_tag="ACHKYO_RS22020"
                     /old_locus_tag="pgaptmp_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HGU4431138.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9011..10255)
                     /gene="sstT"
                     /locus_tag="ACHKYO_RS22025"
                     /old_locus_tag="pgaptmp_004405"
     CDS             complement(9011..10255)
                     /gene="sstT"
                     /locus_tag="ACHKYO_RS22025"
                     /old_locus_tag="pgaptmp_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HGU4431139.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2772 a         2582 c         2741 g         2448 t
ORIGIN      
        1 catttcttga tccagcaagt ctatataaca agctggaact ggatgtagac gatcagaaaa
       61 tcgcgcatat agtgaaagaa aaattatcgc agccgctagc tcgccacggt gaattggctt
      121 gcggttatgt acgtactgac tcagggtgtg gttttctgaa taccgatacg ctggaaataa
      181 tctataatga tgaagaaagt tcggcaacgt tgtttattaa tccgcaatgg aattcagctt
      241 tcgatgcgaa gtcattatat ttaaatccag acaaaaatac tgttaatgct tttatacatc
      301 agcaagacat caatgttctg gcacaggatg attaccaatc gttgtctatt cagggaaacg
      361 gtgcgctggg aataacagaa aatagttata ttggtgcaca ctggaatttc aacggttatg
      421 atgcagatga tgtcagtgac agtaatgctg acgtcagcga tctctattat cgttatgatt
      481 ttttacgtcg ttattatgtg caggcgggtc gcatggacaa ccgcacacta tttaatgcac
      541 aaggcgggaa ctttaccttt aactttctgc cactcggtgc aattgatggg atgcgtatcg
      601 ggtcgacact gagctattta aaccaggcgc aaagccagca aggaacccca gtaatggttc
      661 tgctttcgcg caattctcgt gttgacgctt atcgtaatga gcaacttttg ggatcgtttt
      721 atctcaatag tggttcgcaa tttattgata ccagttcctt tccgccaggc agctacagcg
      781 tggcattaaa agtctacgaa aataaccaac tcacccgcac cgagcttgtg ccgtttacca
      841 aaaccggcgg tctgactgac ggaaatgcgc aatggttctt acaggcaggt aaaactacat
      901 cacaggtttc tgatgatgaa agttcagctt atcagctagg agtacgcctg ccattacatc
      961 cgcaatatga gctctacgca gggctggcga atgccgatga tgtgagtgct ttcgagttag
     1021 gtaataactg gacggcagat ttaggcgggg tggggaatct tgcaatcagc gccagcgtgt
     1081 tccgtaacga tgacggcggc aaaggtgata tgcaacaggc caactggagt aatccgggat
     1141 ggccgacgtt gggcttttat cggaccaact ctgacggcga tgcttgtaca accgacagca
     1201 gagagagcta taacgcctta agctgttatg aaagtatttc cgcgacggtt tcacagaatt
     1261 ttgtcggctg gaatatgatg ctgggttata cccgcacaca aaataacact gatgatagtt
     1321 tgcgttggga taaacagcag agctttgaaa ataactatct tcgccagaca acagcgcaaa
     1381 gtatctctga aactgtacaa cttagcgctt cccgcgcttt tgtgatgcgt gactggattt
     1441 tgagtacgtc agtcggtgtt ttccatcgta atgacaacgg tggcgataac gacgacaacg
     1501 gcttgtactt atcgttttcg ttatctgaca cgccaacgat ggatagcaat aacaacagcc
     1561 attcaacaaa tgtgtctacg gattatcgtt atagcgaaca ggatggcgat caaacgtcat
     1621 ggcagttatc gcataccttt tataacgatt cattcagcca taaagaactt ggcgtaaccg
     1681 tcggaggcct gaacaccgat accataaaca gcgcggttaa cgggcgttgg gatgggcaat
     1741 acggaaatgt ctacgctacc gtatctgata gttatgaccg taagaatcat gatcatcttt
     1801 cggcctttac gggcacttat agctctacgc tggctgtcag tcgctatggc gttaatttgg
     1861 gagccagtgg tacagacgat ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg
     1921 aacaggatga agagagtcag gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt
     1981 tgcagcttgg tcaaagtgac agtgtgttgt tcccttatcc tggatttcag tctggttttg
     2041 ttgaggttaa cgacagtagc cagggcaatc agcaggggac aacaaacatc attaacggtg
     2101 cagggaatcg tgaattaatg ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca
     2161 gctttaacta caactatatc ggtcgtttat tactgccggc agcggtgaaa aaattcccga
     2221 ttgtggggct gaacagcgcc atgttactgg tagctgaaga tggcggattt acacttgaaa
     2281 ttaacggcag cgaaaaagag ctctatctgc tttccgggca gcaattcctt aagtgtccac
     2341 tgagtgttgt aaagaaacgc gccagcattc gttacagcgg agatgttact tgtagtgtgg
     2401 tgacttattc acaattaccg gagtcgattc aggttcaggc acagttgaaa cagcctaaat
     2461 tacgtggaaa cgttcagacg gcgcaaaggg aggttgcacc atgagaaatc gattgattgc
     2521 ggcgatattg ggcttgtttg gcacgctcac tggcgttcag gcagctcctg acgtgaccag
     2581 tgaaattacg tatgatttgg catctggtag agcggattat tacttctgga aggatgaagc
     2641 gtcggcagga aataatggat atatgtggta tgaatgttcg tatcctgacc tccaacaaac
     2701 ctgtacagct aatggaaata tatcgacagt acaaatctat ttaactgaac aacgcagtgg
     2761 gatgcgttgg ccggtaaaac tcaaaggatt taaaacagcc attgtaagta gcgatgaagc
     2821 gccgccagga tgcaaggggg gcaaagggct tcagacgaat cttaaggatt ctaatagatc
     2881 ttcatgtaca gaagatggtc aacattatta tatatacgat acaaagtttc ttacgctcta
     2941 ccttgagcag acagagatga agaatttgcc gattggtggc gtctggaagg ggaaagttaa
     3001 attacattcg aacagcccgg cccaggacta tttcgcaaat attaccctga atacgctcga
     3061 ccccaaccat attgacgtgt tcttcccgga gttcgcccac gccacgccaa gggtgcagtt
     3121 agacttgcat ccaacaggaa gcgttaacgg cagcaactac gcgcaagatc tgaccatgtt
     3181 ggacatgtgc ctgtacgatg gttttaacgg taatgccatc agttatgaaa tcatgctcaa
     3241 agatgaaggg cgaccagctg cagggcgcag agacggttac ttctctatct atcgtcaggg
     3301 agggaccacc accgacgagg gagaacgcat tgattaccgg gtcaaaatgt acaacccgga
     3361 aaccggtggg caaattgatg tgcgcaataa tgaaaatatg gtctggaaca gcattaacct
     3421 gaaacgtgtg cgtccggtgg tactgcccgg tattcgctat gccgtgatgt gtgtgccgac
     3481 acccttgact ctggcagtcg ataaattcag cgtgatggat aaacaggccg ggtactacat
     3541 gggcaaattg tcagtaatct ttacgccttc cttgccaacc atcaattaac cctgcaaacc
     3601 ggacaaacgc ttttttctat ataaacgacg aagccgtctg ataaggcggc ttcgtcatta
     3661 caacttaagt taaacgttaa tgttgcggtg cgggatcgtt atgtgtttcc tgcgccacct
     3721 caatcgccgg tttgttctgc aacacggtcc agataaccag cgcacctaac aggtcaaata
     3781 ctgccagaac tgcgaacagc gggctgaagc cgatggtgtc agccagtgca ccgacaacca
     3841 gcgcaaacag cgtacttgcc agccatgcgg acatcccggt taaaccgttc gccgtcgcca
     3901 cttcgttacg accgaacacg tcagaagaga gcgtaatcag cgcgccagac agtgcctggt
     3961 gggcaaaacc accgatacac agcagcataa ttgcgacata cgggttggtg aacagaccga
     4021 tcatacccgg gccaatcatc agcacagcac ccagcgttac gaccatctta cgggaaacga
     4081 tcaggttcac accaaaccaa cgctggaaca gcggcggcag gtaaccaccg aggatacaac
     4141 cgaggtcagc aaacagcatc ggcatccagg cgaacatcgc gatctctttc aggttaaagc
     4201 cgtaaacttt aaacatgaac agcgggatcc acgcgttaaa agtaccccag gccggttctg
     4261 ccaggaaacg cggcagcgcg ataccccaga actgacggtt acgcaggatc tgaccaacgg
     4321 acattttctt cgccgtgctc acctggtgct gggcttcctg accattaata atatagtcgc
     4381 gttcttcatc ggtcagatgc ttctggtcgc gtggatgttt atagaaaatc agccatgcca
     4441 tcgcccagat aaagctcaat gcaccggaga tgataaatgc catctgccag ctgtgcatta
     4501 cgattgccca taccaccagc ggcggcgcaa tcatcgcacc aatcgaagaa cctacgttaa
     4561 agtaacctac cgcgatggaa cgctctttcg ccgggaacca ttcggagctg gctttcagac
     4621 ccgccgggat catcgctgct tccgcggcac cgactgcacc acgagcaatc gccaggccac
     4681 cccagctacc agccagcgcg gttgcaccac agaacacggc ccacagcaca gcaaacattg
     4741 cataaccgat tttcgtaccc agcacatcca gtacatagcc cgctaccggt tgcatgaccg
     4801 tataagcagc agaataggct gcgatgatat aggaatactg ttgggtggag atgtttaact
     4861 cttccatcag agttggcgca gctgccgcca cagtgttacg cgtcaggtaa ccaagcacgg
     4921 tgccaagcgt caccagtgcg atcatatacc aacgtaaccc tttaatttta cgcatgtaaa
     4981 acctcatcgt tttgttatat ccgcaggaag cgcggccatc atcccgccag aggctgaaga
     5041 tgttagttac gaccgggtaa ccgaaaaaaa accggcacgc cccgaacgtt gccatgaaag
     5101 tttcgtgcta attcggcttc cgtaccggtt aacccgattc atctaacaaa tgaatgaatc
     5161 ggcaatttgc attccgtcga cttatgattt gcgacggcag aaagataact tgtcatacaa
     5221 ctttaaaagg tgagagccat cacaaatgtg cgaatatttg tagggacatt acctgacgac
     5281 agcgaggcca gtactggcgc ggcctgcaga gagatttacc actttgagag taattttttt
     5341 aactacgttt attgatctaa ctcacgaaaa tatcttcgga ctctggaaat tggtgtgata
     5401 actttgtcag catcgcacca taagcaagct agctcactcg ttgagaggaa gacgaaaatg
     5461 actccgttta tgactgaaga tttcctgtta gataccgaat ttgcccgccg tctgtatcac
     5521 gactacgcaa aagaccagcc gattttcgat taccattgcc atttgccgcc gcagcagatt
     5581 gcggaagact atcgttttaa aaacctgtat gacatctggc tgaaaggcga tcactacaaa
     5641 tggcgcgcta tgcgtaccaa cggtgtggcc gagcgtctgt gtaccggtga tgcgtctgac
     5701 cgtgaaaaat ttgacgcctg ggcggcgact gttccgcaca ctatcggcaa cccgttatac
     5761 cactggacgc acctcgaact gcgtcgtccg tttggtatca ctggcaaatt gctttctccg
     5821 tcaactgccg atgaaatctg gaacgaatgt aacgaactgc tggcgcagga taacttctcc
     5881 gcgcgcggca tcatgcagca gatgaacgtg aaaatggtcg gcaccaccga tgacccgatc
     5941 gattctctgg agcatcacgc agagatcgcc aaagacggct ctttcaccat caaagtgctg
     6001 ccgagctggc gtccggacaa agccttcaac atcgaacagg cgacctttaa cgactacatg
     6061 gcgaagctgg gcgaagtttc cgataccgac attcgccgct ttgctgacct gcaaactgcc
     6121 ttgaccaaac gtctggatca cttcgccgct cacggctgta aagtgtctga ccatgcgctg
     6181 gacgtggtga tgtttgctga agcgaacgaa gcggaactgg acagcattct ggcgcgccgt
     6241 ctggctggcg aacccctgag cgagcacgaa gtggcacagt tcaaaactgc ggtactggtg
     6301 ttccttggtg ccgaatatgc acgtcgcggc tgggtacagc agtaccacat tggcgcgctg
     6361 cgtaataaca acctgcgtca gttcaaactg ctggggccgg atgtaggctt tgactccatc
     6421 aacgaccgtc cgatggcgga agagttgtct aagctgctga gcaagcagaa cgaagaaaac
     6481 ctgctgccga aaaccattct ctactgcctg aacccgcgcg ataacgaagt gctgggcacc
     6541 atgatcggta acttccaggg cgaaggtatg ccgggcaaaa tgcagttcgg ttccggctgg
     6601 tggtttaacg atcagaaaga cggtatggaa cgtcagatga cccaactggc gcagctcggt
     6661 ctgctgagcc gctttgtcgg tatgctgact gacagccgta gcttcctgtc atacacccgt
     6721 cacgaatact tccgccgcat tctgtgccag atgatcggtc gctgggtgga agcgggcgaa
     6781 gcaccggcgg acatcaacct gctgggcgag atggtgaaaa atatttgctt taacaatgcg
     6841 cgtgactact tcgccattga actgaactaa ggtctggggt tgatatgcaa tacatcaaga
     6901 tccatgcgct ggataacgtc gcggtcgctt tagcagattt ggctgaaggc acagaagtca
     6961 gtgtcgataa ccagactgtt acgctgcgcc aggatgttgc tcgtggacat aaatttgcgt
     7021 taacggatat cgcaaaaggg gccaacgtca tcaaatatgg cctgccgatt ggttatgcat
     7081 tggcggatat tgcggcaggt gttcacgttc acgcccacaa tacgcgcacg aatctgagcg
     7141 atctggatca gtatcgctat caacctgatt ttcaggatct gcctgcgcaa gcggcagatc
     7201 gtgaagtgca gatctatcgt cgcgctaacg gcgatgtcgg ggtgcgtaat gagctgtgga
     7261 tcctgccaac cgtgggctgt gtcaacggca tcgcgcggca aatccagaac cgtttcctga
     7321 aagagaccaa caacgccgaa ggtaccgacg gcgtgttcct cttcagccac acctacggct
     7381 gctcacaact gggcgacgat cacatcaaca cccgcaccat gctgcaaaac atggtgcgcc
     7441 acccgaacgc gggcgcagtg ctggtgattg gtctgggctg tgaaaacaac caggttgccg
     7501 cattccgtga aacgttgggc gatatcgatc ctgaacgcgt tcatttcatg atctgccaac
     7561 agcaggatga tgagatcgaa gccgggattg agcatttgca tcagctgtat aacgtgatgc
     7621 gcaacgataa acgcgagcca ggcaaactca gcgaactgaa gtttggtctg gagtgcggtg
     7681 gttctgacgg tctttctggt attactgcta acccgatgct ggggcgtttc tctgactacg
     7741 tgattgctaa cggcggtact accgtactga ccgaagtgcc ggagatgttt ggcgcagagc
     7801 agttgctgat ggaccattgc cgcgacgaag caacgtttga aaaactggtc accatggtca
     7861 acgacttcaa acagtacttt attgcccatg accagccgat ctatgaaaac ccatcgccgg
     7921 ggaacaaagc gggcggtatc accacgctgg aagataaatc ccttggctgt acccagaaag
     7981 cgggttccag cgtcgtggtt gacgtgctgc gttacggcga gcgtctgaaa acgccggggc
     8041 tgaacttgtt aagtgcgccg ggtaacgatg ccgtagcgac cagcgccctg gcgggtgcgg
     8101 gctgccatat ggtgctgttc agtactggtc gtggcacgcc atacggcggc tttgtaccga
     8161 cggtgaaaat cgctaccaac agtgaactgg cggcgaagaa aaaacactgg atcgactttg
     8221 acgcgggtca gctgatccac ggtaaagcga tgccgcagtt gctggaagaa tttatcgaca
     8281 ccatcgttga gtttgccaac ggtaaacaaa cctgcaacga gcgtaacgac ttccgcgaac
     8341 tggcgatctt caaaagcggc gtaacgctat aaagtcgaaa tgagttgtta ataaaaaacg
     8401 gcgtttcata atgtgaagcg ccgtttgctt ttccatacct gataaggatt tatcatgacc
     8461 gcgtattggc tggcccaggg cgtgggtgtc atcgcctttc tgattggtat cacaacattt
     8521 ttcaatcgtg acgaacgtcg cttcaaaaag cagctttcgg tctatagcgc cgttattggc
     8581 gtacattttt ttcttctggg cacctatccc gctggtgcca gtgccatcct taatgccatt
     8641 cgtacattga ttaccttacg cacgcgcagc ttatgggtaa tggcgatttt tattgtgctg
     8701 actggcggaa ttggcctcgc gaagttccat catcctgtcg aactattgcc ggttatcggt
     8761 acgattgtca gtacctgggc gctgttccgc tgtaaagggc tcaccatgcg ctgcgtgatg
     8821 tggttttcaa cgtgttgctg ggtgattcac aacttctggg cggggtcgat aggcggcaca
     8881 atgattgagg gtagttttct gcttatgaat ggcttgaata tcattcgttt ctggcggatg
     8941 cagaaaaggg gaattgatcc gtttaaagta gagaaaaccc ctcccgccgt agacgaaagg
     9001 ggttaaacaa ttaattacgc agggcgctat ttgccagacg atcgtcttcc gcctggcaag
     9061 ctgccgcagt gaacagcacg tcagttgaag agttcagcgc ggtttcgcaa gagtcctgca
     9121 atacgccgat gataaagccg acggcaacca cctgcatggc gatatcgttc gaaataccga
     9181 acatattaca ggccagcggg atcagcagca gagaaccccc cgccacgccg gatgcgccac
     9241 aggcacacag agaagccacc acgctcaaca gcagcgccgt gggcagatcg accgggatac
     9301 ccagcgtatt aaccgcagcc agcgtcaaca cggtaatggt gattgctgcg cccgccatat
     9361 tgatggtggc acccagcgga atagaaacgg aataggtatc gcgatccaga ttcagctttt
     9421 cacacagtgc catattcacc ggaatgttcg ccgcagagct gcgggtgaag aaggcataca
     9481 cgccgctttc gcgcaggcac agcagcacca gcgggaacgg gttacgacga attttccacc
     9541 acaccagcaa tgggttaacc accagcgcca ccagtaacat acagccaacc agcacgacca
     9601 gcagttgcgc gtagccccac agtgtggaga aaccggtggt tgccagggta gaagaaacca
     9661 gaccaaaaat accaatcggt gcgaagcgaa tgaccagttt caccataaag gtgacggcat
     9721 tcgacatatc gttgaccagg tttttggtgg tctcgttacc gtgacgcagt gcgaaaccga
     9781 ggccaattgc ccacaccagg atcccgatgt agttaccttt cagcagcgcg tcgatggggt
     9841 tggaaaccat gctcattacc agcccgcgca tcacttcgac aatgcctgac ggcggcgaaa
     9901 tatcacccgc gctactggat aaatgcaggg tagaagggaa ggcaaagctg aagactactg
     9961 cggccagagc agcagagaag gtacccagca gatagaggaa caaaataggg cggatattgg
    10021 ttttctgccc gtgctggtgg ttagcaatag atgccatcac cagcatcaac accaggatgg
    10081 gggcaacggc tttcagtgcg ccgacgaaca aagtacctaa cagaccaaca gcttccgccg
    10141 cgggttttga gatccatgcc agaagaatcc ccagaacaag gccgaccagg atttgtttta
    10201 ccaggctgcc atgagccaga cgccggaata gccccggtga acgttgcgta gtcatttttc
    10261 gatcctttca ttgtgttgtc gcacatccct ggtgcgttct gtcgacgcat ctttcaggat
    10321 gtaacaaagt gtttgcaagg tcgagtataa ggaaagttac agcttcggga agcgaaaaat
    10381 gctggatttt aaagagaggt catattttgc aacttaattg ttacatttgt ttgacataaa
    10441 cagagggaag gactggctac taaggctgat tagataacat atgggcaaaa ctatttggaa
    10501 tatgagaacg gttgcgctcc tttaatgtgt tatgccatat aaa
//
LOCUS       DAZGAN020000150        10403 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGAN020000150 DAZGAN020000000
VERSION     DAZGAN020000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.150"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(291..1223)
                     /locus_tag="ACHKYO_RS22030"
                     /old_locus_tag="pgaptmp_004406"
     CDS             complement(291..1223)
                     /locus_tag="ACHKYO_RS22030"
                     /old_locus_tag="pgaptmp_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGU4431140.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1517..2272
                     /gene="mlaA"
                     /locus_tag="ACHKYO_RS22035"
                     /old_locus_tag="pgaptmp_004407"
     CDS             1517..2272
                     /gene="mlaA"
                     /locus_tag="ACHKYO_RS22035"
                     /old_locus_tag="pgaptmp_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HGU4431141.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2454..3512)
                     /gene="yfdF"
                     /locus_tag="ACHKYO_RS22040"
                     /old_locus_tag="pgaptmp_004408"
     CDS             complement(2454..3512)
                     /gene="yfdF"
                     /locus_tag="ACHKYO_RS22040"
                     /old_locus_tag="pgaptmp_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HGU4431142.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTE
                     YQNEINQLSAPGVNFDDI"
     gene            complement(3879..5219)
                     /gene="fadL"
                     /locus_tag="ACHKYO_RS22045"
                     /old_locus_tag="pgaptmp_004409"
     CDS             complement(3879..5219)
                     /gene="fadL"
                     /locus_tag="ACHKYO_RS22045"
                     /old_locus_tag="pgaptmp_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HGU4431143.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            5591..5875
                     /locus_tag="ACHKYO_RS22050"
                     /old_locus_tag="pgaptmp_004410"
     CDS             5591..5875
                     /locus_tag="ACHKYO_RS22050"
                     /old_locus_tag="pgaptmp_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HGU4431144.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6055..7365
                     /gene="fadI"
                     /locus_tag="ACHKYO_RS22055"
                     /old_locus_tag="pgaptmp_004411"
     CDS             6055..7365
                     /gene="fadI"
                     /locus_tag="ACHKYO_RS22055"
                     /old_locus_tag="pgaptmp_004411"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HGU4431145.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7365..9506
                     /gene="fadJ"
                     /locus_tag="ACHKYO_RS22060"
                     /old_locus_tag="pgaptmp_004412"
     CDS             7365..9506
                     /gene="fadJ"
                     /locus_tag="ACHKYO_RS22060"
                     /old_locus_tag="pgaptmp_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HGU4431146.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPALLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDL"
     gene            9712..10197
                     /gene="sixA"
                     /locus_tag="ACHKYO_RS22065"
                     /old_locus_tag="pgaptmp_004413"
     CDS             9712..10197
                     /gene="sixA"
                     /locus_tag="ACHKYO_RS22065"
                     /old_locus_tag="pgaptmp_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HGU4431147.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2582 a         2385 c         2630 g         2806 t
ORIGIN      
        1 gagcgggata tgaaaattgc cgtggatcgg tttaaccgcg agtacaacaa aaaagtggga
       61 gtggagtaaa gaacatgccc cgtacggaag tgcggggcat gcttaacggg atgtgtacat
      121 aaatgggtac tgatatgacg attatttcgg tgctttctgt atttaaatca attagttaat
      181 acttgtattc attatatcca tttaactaag aggacaatgc ggcatgagta tacccgctaa
      241 tggactgcgg ggtaagtacg ctgccgctcg attgcttaaa ccctcgccat ttatgctggg
      301 tttttatcat ttttcttaat gttttccgca cgttctgctt tttggcgtgc ttctgcttta
      361 cgcttgttgc tcatgtcgtt acgaatctgt gcatgactca ttaacgcgaa gataaaggtg
      421 ccgccgcaga tgttccccgc taaagtaggt agtgcgaagg gccagatgaa atcgctccag
      481 tgcagcgtgc cgttaaacac cagatagagg atttcaacag aaccgacaac gatgtgggtg
      541 gtgtcaccca gtgcaataag ccaggtcatc aaaataatca ccacaatctt tgccgcaccc
      601 gctgcaggaa acatccaaac catagtggcg atcagccagc cggaaatgat cgcgttggca
      661 aacatctcgc tgggggtgtt cttcatcaca tccatgccga ttttgacaaa tgcatcgcga
      721 gtttcttcat tgaagatagg catatattca aatgcccatg cagcaatacc tgtcccgaga
      781 atattaccca gcagcacgac gccccataat cgcataagta agccgacgtt gctcattgtc
      841 ggtttttgca tgacgggtag taccgcagtc acggtgtttt cggtaaataa ttgctggcgg
      901 gccataatga cgataataaa accaaaggta taaccgagat tctccagtaa gaagctgcct
      961 ggcactcctt ccagttcgac atgaaatatc ccttttgcca gtagcgaagc gcccatcgac
     1021 agacccgccg caatggctga ccacagtagc gccattgcgt cgcgttccag ctctttttca
     1081 ccatcctggc ggatatgctc atgaattgcc atcgcccggg aggggagtcg gtcttcatct
     1141 atttctattt ttttgccgcg ctctttttct tcgctctcaa cttcaatttc gtcgctgtgt
     1201 tgatcaattt tgtcgttgtc catggtctct tctcttgaat tagcacgtat agctaaagcg
     1261 tagcggcttt tttgctcgca actggcggga gttactctga aaatgtagaa aaggctgcgt
     1321 ttgccttttt ctgtttctat agaatcaagt agcctacagg gcggcgatta ccaggctatg
     1381 atcaaatcag caaatcaggg cgtctggaca tcagttgacg tgctgttaca atcgcccaca
     1441 cctaaacagg cggatacggt atcgttccgt catggatggc aaactgcata agccataaaa
     1501 aaacagggag acatttatga agcttcgcct gtcggcgctt gctctgggaa ctacgcttct
     1561 ggtggggtgt gcgagttccg gtacagatca gcaagggcgt tctgacccgt tagaagggtt
     1621 caaccgcacc atgtacaact tcaacttcaa tgtattagac ccgtatattg ttcgaccggt
     1681 cgctgtcgcc tggcgtgatt atgttccgca accggcgcgt aacggtttga gcaactttac
     1741 tggcaacctt gaagaacctg cggtgatggt taactacttc ttgcagggcg acccttatca
     1801 ggggatggtc cactttaccc gctttttcct gaacaccatt ttggggatgg gcggttttat
     1861 tgatgttgca gggatggcga acccgaaact gcaacggact gaacctcacc gcttcggtag
     1921 tacgcttggt cattatggcg tgggttatgg gccttacgtc cagttaccgt tctacggtag
     1981 cttcacgctg cgtgatgacg gtggtgatat ggcggatggt ctttacccgg ttctttcctg
     2041 gctgacctgg ccgatgtctg tgggtaaatg gacgcttgaa gggatagaaa ctcgtgcgca
     2101 gttgttggat tccgatggtc tgctgcgtca gtcgtccgat ccttatatta tggtgcgcga
     2161 agcgtacttc cagcgtcatg atttcatcgc taatggcggc gaactcaaac cgcaggaaaa
     2221 tccgaacgcg caagcgattc aggatgattt aaaagatatc gattctgaat aagaaacaaa
     2281 taaaaaaggt gagtcgcaat actcaccttt tttgttgtta tccctcaatt accacaagta
     2341 aaaggagata aaccgacaat gacccggacg taatgatgta aaaatactga tgttcgtcac
     2401 tgactatttc cgatattcag gagttgaaaa atcaaaaaac tggaaaaata attttaaata
     2461 tcatcaaaat taacacctgg cgcagatagt tgattaattt cattttgata ttccgtctgg
     2521 cgattttgaa gccttttttc aactttatct tcgagatcct taaagtcttt gtatttattt
     2581 tcaggatggt caacgtcaac tttgttagcg aataaagtag aaattatttc ttttacatta
     2641 tcgcgtctaa attttgcatc gtccacttga aaattatatc ttattgctcg acctaaacgt
     2701 ttaaaaactt cgttaggttc tctcagtctt tccccgttga gctcgtttac aacctgatga
     2761 tcggaacctg ttgcaaatga ttctgccatg atagctttgc tgctttcaaa ccacgctgaa
     2821 gcactcctgg cgttttcact acaatattgg gtgagactat caccattaat acgaattcct
     2881 ctttgaatgt ggtagtcgtc ctgatcgtag taaccgagag ataagtaatc atttccgttt
     2941 ttatttttaa ttacaatagc gagagagtcg ccttttttat ctttaccgta ccaggtaaat
     3001 aagtccgttg gttcgctatt cccgctctca tcaaattccg ttaccgtatg tctgagtttg
     3061 tgtgatatct tatcgaagat aaaaccagga tctccatcat atatttcttt taatgcgctg
     3121 aagatcattt ccttgttatt gatatcatga gtcttctcaa ctatgttgtc attaatgata
     3181 gtacggacga gttctttgtc tttatagttc gcaggtaatt ctattggttc ttggttgtcg
     3241 ataccaatag taatatggtc tgtatcattg gagccaatat caatataagt aactgaggaa
     3301 ttattaatgt taaatctata aggatcgtcg tttactcgta tggcgttttt tataagctca
     3361 agtagatgtt taatacaggt gcttattcct tccttaccat taaaaaggtt tttgagctct
     3421 tgtactaatc cattaacttc atcattgtta ttttcattaa tggattctgg gtaagttgcg
     3481 ctggtattgt tgattgaaat agatggtagc atgattttat tccattttga ttgatttgtt
     3541 acaaatatat attacctaat gaaatagact atcaaaaaac gctcatttat ttagaaccta
     3601 tctttaattt gagtgtatga gcgcacaata gccagggggg ggggagtatc ctggaaattc
     3661 agaaaagtaa actctttgag aaggacgagc agaacgcctg aaaatatgcc tttcccttca
     3721 gatttgtctg tggataccgc ttattgattt gaaagggcaa gctgccatca ggctgtttgc
     3781 aaaactcagg aggtaagcaa tgaggacact actttcggtg aagtggggga gttaaagtca
     3841 cctgctatgc aggtgacttt atccaggcga acgtgttatc agaacgcgta gttaaagtta
     3901 gtaccgaaca gccaggcttt accttcagac tcgaactggt atgggccttc gttaattttc
     3961 acgctctgac cgtgcatata agaaacacca acgtcgactg aagcatcttt attaaatgcg
     4021 taagtcgtac ctgcactcag ccagaaacgg tcctggtccg gaatggagat agaacgattc
     4081 tgagccggaa ctgggctgtc atcaaaggcg ataccggtac ggaaggtcca gttatcatcg
     4141 tagtaataag tggtacccaa cgcgatgcgg taagcatctt taaagccttc atgtttctgg
     4201 aacagcgtgt cgccactggt tgaggtcgct ttcagctgct ggaactgact ccagctggtg
     4261 taagccaggc tatagtgaat cgcccactgt ggatcaacac ggttataacc tgacacttcc
     4321 cacatttcag gcaggttcag cgtcagataa cccgattgcg ttgcgccacc tgtcgcggta
     4381 ggaattggca aaccgtagtt attaaacgca cgattaagat cgctgctgta gttacctttg
     4441 aagtcaattt tcacttcaga acggtaggtc agtgcatagc ggttattttt atccagttca
     4501 tacaggattc cggcgttcca gccaaagccc cactggttac cgttcagatg agcgattttg
     4561 gtattactgt cgataccgtt ggcggtagct gccaatgctt gcccttgagg agtttgcccg
     4621 gcaggagatt gcataatctg accagcaacc aactgcccca gatcgcctgc gaaacgttca
     4681 attttcgcgc gagcgtagac ggcgttgaaa ccaagaccaa agctccatgc attatttaag
     4741 cgatacgcac cgcttaagtt caggttcatg gtttcaaggt cagttgtacc cccgacagag
     4801 ccgcctgcat aagtatcatt aaactctgtt gccagaccat agttagaggt aatagaagcg
     4861 ccccaaccaa attggtcgtt aatcggtgca acaaagtgca tgttcggaac ccatgccgta
     4921 ggcgcgatgt tatcggcttt caggctacga ccagacggag acgttccgct gatattcaca
     4981 tccgggtcaa tataaaccgc acccgcagaa aatgtcgggc ggtcaaacat ggtaatcaat
     5041 gcggggttac ggctaacgtt acctgcatca tcggcaattg cgccttcccc tgaataagcc
     5101 cggcccaggc cagaggaaga aaattcgttt aactgaaagc ctgccgacca ggcctgggtg
     5161 gagataagtg ccactgcgac tgcgagagca gactttgtaa acagggtttt ctggctcatg
     5221 accataacct caatgattta tttttataca aactatgtta cgtgctgtaa caggagcgcg
     5281 aagtgtaggg tctgaggtac atcttagaaa tcagaccagt ggcgagagta taggtcggac
     5341 cagctggaat gttgcaagta tgtttccatc ttttttcaaa tatgatctaa gaaacgcgat
     5401 ccagctggca aaatttggag gttgcgcact cgcggattcg ggtgaatttt gttttagatc
     5461 atttttaagt gtgatttcgg tcacttatcc gattttgcag aattaacaac tggagcagca
     5521 ctttccggaa gcgtaaaata tccacaacgt ttatctggca ccttgggtgc acatacagag
     5581 gaaatcaacg atgagtaaat gtagtgctga tgaaaccccg gtttgctgct gtatggatgt
     5641 tggcaccatt atggacaact ccgactgcac cgcgtcttac agccgcgtgt ttgcaaaccg
     5701 cgcagaagca gaacaaacgc tggcggcgct gactgaaaaa gcccgtagcg tggaatccga
     5761 accgtgcaaa atctccccga ctttcactga agagtctgac ggtgtgcgcc tggatatcga
     5821 tttcactttt gcctgcgaag ctgaaatgct gatcttccag ctgggtctgc gttaattcct
     5881 ctcgggtacg ttcgcccctg tcagttgctg gcaggggcgt tttcttttct ttctcctgtc
     5941 ttcactgtgt catagctccc actttccttt ttctccgctt ggctaaatgt aaaaaaatgg
     6001 ttaagactat gatcaggtca gaccacttta tttatttttt tacaggggag tgttatgggt
     6061 caggttttac cgctggttac ccgccagggc gatcgtatcg ccattgttag cggtttacgt
     6121 acgccttttg cccgccaggc gacggctttt catggcattc ccgcggttga tttagggaag
     6181 atggtggtag gcgaactgct ggcacgcagc gagatccccg ctgaagtgat tgaacagctg
     6241 gtctttggtc aggtcgtaca aatgcctgaa gcccccaaca ttgcgcgtga aattgttctc
     6301 ggtaccggaa tgaatgtgca taccgatgct tacagcgtca gccgcgcttg cgctaccagt
     6361 ttccaggcgg ttgcaaacgt cgcagaaagc ctgatggcgg gaactatccg agcggggatt
     6421 gccggtgggg cagattcctc ttccgtattg ccaattggcg tcagtaaaaa actggcgcgc
     6481 gtgctggttg atgtcaacaa agctcgaacc atgagccagc gactgaaact cttctctcgc
     6541 ctgcgtttgc gcgacttaat gcccgtaccg cctgcggtag cagaatattc tactggcttg
     6601 cggatgggcg acaccgccga acaaatggcg aaaacctacg gcatcacccg agaacagcaa
     6661 gatgcattag cgcaccgttc gcatcagcgt gccgctcagg cgtggtcaga cggtaaactc
     6721 aaagaagagg tgatgactgc ctttatccct ccttataaac aaccgcttgt cgaagacaac
     6781 aatattcgcg gtaattcctc gcttgccgat tacgcaaagc tgcgcccggc gtttgatcgc
     6841 aaacacggaa cggtaacggc ggcgaacagt acgccgctga ccgatggcgc agcggcggtg
     6901 atcctgatga ctgaatcccg ggcgaaagaa ttagggctgg tgccgctggg gtatctgcgc
     6961 agctacgcat ttactgcgat tgatgtctgg caggacatgt tgctcggtcc ggcctggtca
     7021 acaccgctgg cgctggaacg tgccggtttg acaatgagcg atctgacatt gatcgatatg
     7081 cacgaagcct ttgcagctca gacactggcg aatattcagt tgctgggtag tgaacgtttt
     7141 gctcgtgaag tactggggcg tgcacatgcc actggcgaag tggacgatag caaatttaac
     7201 gtgcttggcg gttcgattgc ttacgggcat cccttcgcgg cgaccggcgc gcggatgatt
     7261 acccagacat tgcatgaact tcgccgtcgc ggcggtggat ttggtttagt taccgcctgt
     7321 gctgccggtg ggcttggcgc ggcaatggtt ctggaggcgg aataatggaa atggcatcag
     7381 tgtttactct taatgttcgt ctggacaaca ttgctatcat caccatcgac gtaccgggtg
     7441 agaaaatgaa taccctgaag gcggagtttg cctcgcaggt gcgcgccatt attaagcaac
     7501 tccgtgaaaa caaagagttg cgaggcgtgg tgtttgtctc cgctaaaccg gacaacttca
     7561 ttgctggcgc agacatcaac atgatcggca actgcaaaac ggcgcaagaa gcggaagcac
     7621 tggcgcggca gggccaacag ttgatggcgg agattcatgc tttgcccatt ccggttatcg
     7681 cggctattca tggcgcttgc ctgggtggtg ggctggagtt ggcgctggcg tgccacggtc
     7741 gcgtttgtac tgacgatcct aaaacggtgc tcggtttgcc tgaagtacaa cttggattgt
     7801 tacccggttc aggcggcacc cagcgtttac cgcgtctgat aggcgtcagc acagcattag
     7861 agatgatcct caccggaaaa caacttcggg cgaaacaggc attaaagctg gggctggtgg
     7921 atgacgttgt tccgcactcc attttgctgg aagtcgctgt tgagctggcg aagaaggatc
     7981 gcccatcttc ccgccctcta cctgtacgcg agcgtattct ggcggggccg ttaggtcgtg
     8041 cgctgctgtt caaaatggtc ggcaagaaaa cagaacacaa aactcaaggc aattatccgg
     8101 cgacagaacg catcctggag gttgttgaaa cgggattagc gcagggcacc agcagcggtt
     8161 atgacgccga agctcgggcg tttggcgaac tggcgatgac gccacaatcg caagctttgc
     8221 gtagtatctt ttttgccagt acggaagtga agaaagatcc cggtagtgat gcaccgcctg
     8281 cgctattaaa cagcgtggga attttaggtg gtggcttgat gggcggcggt attgcttatg
     8341 tcactgcttg taaagcgggg cttccggtca gaattaaaga tatcaacccg cagggcataa
     8401 atcatgcgct gaagtacagt tgggatcagc tggagggcaa agttcgccgt cgtcatctca
     8461 aagccagcga acgtgacaaa cagctggcat taatctccgg aacgacggac tatcgcggct
     8521 ttgcccatcg cgatctgatt attgaagcgg tgtttgaaaa tctcgaatta aaacaacaga
     8581 tggtggcgga agtggagcaa aattgcgccg ctcataccat ctttgcttcg aatacgtcat
     8641 ctttgccgat tggtgatatc gccgctcacg ccacgcgacc tgagcaagtt atcggcctgc
     8701 atttcttcag tccggtggaa aaaatgccgc tggtggagat tattcctcat gcgggtacat
     8761 cagcgcaaac catcgccact acggtaaaac tggcgaaaaa acagggcaaa acgcccattg
     8821 tcgtgcgtga caaagccggg ttttacgtca atcgcatctt agcgccttac attaatgaag
     8881 ctatccgcat gctgaccgaa ggtgaacggg tagagcacat tgatgctgcg ctagtgaaat
     8941 ttggttttcc ggtaggccca atccaacttt tggatgaggt aggaatcgac accgggacta
     9001 aaattattcc tgtactggaa gccgcttatg gagaacgttt tagcgcgcct gcaaatgttg
     9061 tttcttcaat tttgaacgac gatcgcaaag gcagaaaaaa tggtcggggt ttctatcttt
     9121 atggtcagaa agggcgtaaa agcaaaaaac aggtcgatcc tgccatttac ccgctcattg
     9181 gcgcacaagg gcaggggcga ctctccgcac cgcaggttgc tgaacggtgt gtgatgttga
     9241 tgctgaatga agcagtacgt tgtgttgatg agcaggttat ccgtagcgtg cgtgacgggg
     9301 atattggcgc ggtatttggc attggttttc cgccatttct cggtggaccg ttccgctata
     9361 tcgattctct cggcgcgggc gaagtggttg caataatgca acgacttgcc acgcagtatg
     9421 gttcccgttt taccccttgc gagcgtttgg tcgagatggg cgcgcgtggg gaaagttttt
     9481 ggaaaacaac tgcaactgac ctgtaataag aaggtcaaag ctatatgaat ccgcgctgaa
     9541 tggcggagtg ttggtcaaaa tgtaaacgca tattgactat acttacgcca ttgaggtaaa
     9601 aaacagcgtt tcattcggtg aatggataag gcacaatgcc ggccaccgtt ttctttctct
     9661 ggtttcagat gaaagaaaac gggcgaatct ggttaacaaa agcggtgcaa tatgcaagtt
     9721 tttatcatgc gtcacggcga cgcagccctc gatgccgcca gtgattccgt tcgtcctctg
     9781 accactaatg gttgtgacga atctcgcctg atggcgaact ggctgaaagg tcaaaaagtg
     9841 gaaatcgaac gcgttctggt gagcccgttc ctgcgagccg agcaaacact ggaagaagta
     9901 ggggattgtc tgaacctgcc ttccagtgca gaagttctgc cggagttaac gccctgcggc
     9961 gatgttggtc tggtcagtgc ctatttgcag gcgttgacca atgaaggtgt tgcttcggta
    10021 ctggtgatct ctcacttacc gttagtcggg tatctggttg ccgagttatg tccgggcgaa
    10081 acgccgccta tgtttaccac ctcggccatt gccagcgtaa cgcttgatga aagcggaaac
    10141 ggtacattca actggcagat gagcccgtgt aatctgaaga tggcaaaagc tatctgatgt
    10201 gttccaaatt atgcccaatg aggtagattc attgggcata attgctcatc aggcagcgga
    10261 tatctgtcaa cctcactact atcgaagact ctccttatct ataaataaaa cactttctac
    10321 attcattcgg aaattattct tctgcgcgta tcttgatttt gcccaaagtt acatgttgaa
    10381 atgaataatg tctgtttgac act
//
LOCUS       DAZGAN020000151        10393 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGAN020000151 DAZGAN020000000
VERSION     DAZGAN020000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.151"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1373
                     /gene="cdgI"
                     /locus_tag="ACHKYO_RS22070"
                     /old_locus_tag="pgaptmp_004414"
     CDS             <1..1373
                     /gene="cdgI"
                     /locus_tag="ACHKYO_RS22070"
                     /old_locus_tag="pgaptmp_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU4431148.1"
                     /translation="SSTHIALLFMVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEN
                     TNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVE
                     TIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTG
                     ILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLL
                     FFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVS
                     FLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFK
                     RINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAER
                     MRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVITEADKALREAKSEGGNK
                     VIIHHI"
     gene            1554..3044
                     /gene="dgcJ"
                     /locus_tag="ACHKYO_RS22075"
                     /old_locus_tag="pgaptmp_004415"
     CDS             1554..3044
                     /gene="dgcJ"
                     /locus_tag="ACHKYO_RS22075"
                     /old_locus_tag="pgaptmp_004415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HGU4431149.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3087..3590
                     /gene="yeaK"
                     /locus_tag="ACHKYO_RS22080"
                     /old_locus_tag="pgaptmp_004416"
     CDS             3087..3590
                     /gene="yeaK"
                     /locus_tag="ACHKYO_RS22080"
                     /old_locus_tag="pgaptmp_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HGU4431150.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEIDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3591..3695)
                     /gene="yoaI"
                     /locus_tag="ACHKYO_RS22085"
                     /old_locus_tag="pgaptmp_004417"
     CDS             complement(3591..3695)
                     /gene="yoaI"
                     /locus_tag="ACHKYO_RS22085"
                     /old_locus_tag="pgaptmp_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HGU4431151.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3865..4311
                     /gene="yeaL"
                     /locus_tag="ACHKYO_RS22090"
                     /old_locus_tag="pgaptmp_004418"
     CDS             3865..4311
                     /gene="yeaL"
                     /locus_tag="ACHKYO_RS22090"
                     /old_locus_tag="pgaptmp_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HGU4431152.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4268..5089)
                     /gene="nimR"
                     /locus_tag="ACHKYO_RS22095"
                     /old_locus_tag="pgaptmp_004419"
     CDS             complement(4268..5089)
                     /gene="nimR"
                     /locus_tag="ACHKYO_RS22095"
                     /old_locus_tag="pgaptmp_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HGU4431153.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKQDPRQ"
     gene            5186..6367
                     /gene="nimT"
                     /locus_tag="ACHKYO_RS22100"
                     /old_locus_tag="pgaptmp_004420"
     CDS             5186..6367
                     /gene="nimT"
                     /locus_tag="ACHKYO_RS22100"
                     /old_locus_tag="pgaptmp_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HGU4431154.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6422..6769
                     /gene="yeaO"
                     /locus_tag="ACHKYO_RS22105"
                     /old_locus_tag="pgaptmp_004421"
     CDS             6422..6769
                     /gene="yeaO"
                     /locus_tag="ACHKYO_RS22105"
                     /old_locus_tag="pgaptmp_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HGU4431155.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6791..7045)
                     /gene="yoaF"
                     /locus_tag="ACHKYO_RS22110"
                     /old_locus_tag="pgaptmp_004422"
     CDS             complement(6791..7045)
                     /gene="yoaF"
                     /locus_tag="ACHKYO_RS22110"
                     /old_locus_tag="pgaptmp_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HGU4431156.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7228..8253
                     /gene="dgcP"
                     /locus_tag="ACHKYO_RS22115"
                     /old_locus_tag="pgaptmp_004423"
     CDS             7228..8253
                     /gene="dgcP"
                     /locus_tag="ACHKYO_RS22115"
                     /old_locus_tag="pgaptmp_004423"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HGU4431157.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGSDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8286..8384
                     /gene="yoaK"
                     /locus_tag="ACHKYO_RS22120"
                     /old_locus_tag="pgaptmp_004424"
     CDS             8286..8384
                     /gene="yoaK"
                     /locus_tag="ACHKYO_RS22120"
                     /old_locus_tag="pgaptmp_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HGU4431158.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8387..8461
                     /gene="yoaJ"
                     /locus_tag="ACHKYO_RS22125"
                     /old_locus_tag="pgaptmp_004425"
     CDS             8387..8461
                     /gene="yoaJ"
                     /locus_tag="ACHKYO_RS22125"
                     /old_locus_tag="pgaptmp_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HGU4431159.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8520..8768)
                     /gene="yeaQ"
                     /locus_tag="ACHKYO_RS22130"
                     /old_locus_tag="pgaptmp_004426"
     CDS             complement(8520..8768)
                     /gene="yeaQ"
                     /locus_tag="ACHKYO_RS22130"
                     /old_locus_tag="pgaptmp_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU4431160.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8995..10203)
                     /locus_tag="ACHKYO_RS22135"
                     /old_locus_tag="pgaptmp_004427"
     CDS             complement(8995..10203)
                     /locus_tag="ACHKYO_RS22135"
                     /old_locus_tag="pgaptmp_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU4431161.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            10211..>10393
                     /locus_tag="ACHKYO_RS22140"
                     /old_locus_tag="pgaptmp_004428"
     CDS             10211..>10393
                     /locus_tag="ACHKYO_RS22140"
                     /old_locus_tag="pgaptmp_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4431162.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRLQIFDHDATEK
                     LRTYFSNVCADFEAELV"
BASE COUNT         2550 a         2512 c         2414 g         2917 t
ORIGIN      
        1 aatccagcac tcacattgct ctccttttta tggtttctat gggtacacaa aaattaaaag
       61 ctcaaagctt ttttattttc agtttattgc tgacgttaat tttattttgc attactacct
      121 tatataacga aaacacaaat gtaaaactca tcccacagat gaattacctg atggttgttg
      181 tggctttgtt tttccttaac gccgtcattt ttcttttcat gttaatgaaa tatttcacta
      241 acaaacaaat tttaccaaca ctcattttaa gccttgcatt tttaagtggc cttatctatt
      301 tagttgaaac cattgtaatt atccataaac caattaacgg cagtacactg atccagacaa
      361 agtcgaatga tgtttctatt ttctatattt tccgccaact cagttttatt tgtttaacct
      421 ctctggcact cttttgttat ggaaaagaca acatccttga caacaataag aaaaaaacgg
      481 gaatcctgtt gctggcgctg attccttttt tagtttttcc ccttctggca cacaatctga
      541 gcagttataa cgctgactat tctttgtatg tcgtcgacta ctgtccggac aaccatactg
      601 cgacctgggg aatcaactat acaaaaatat tggtttgtct gtgggcattt ttactgttct
      661 ttattatcat gcgtacacga ttagccagcg aactatggcc attaatagca ttattatgtc
      721 tggcatcgct atgctgcaac ttacttctac tgactctgga tgagtataat tacaccatct
      781 ggtatatcag tcgcgggatt gaagtttcca gtaaactgtt tgttgtgtct tttctgattt
      841 ataacatttt tcaggagctg caactctcca gcaaactggc agttcatgat gtgctgacca
      901 atatttataa tcggcgctac tttttcaaca gcgtagagtc attattgtcg cgacctgttg
      961 ttaaggactt ctgtgtcatg ctggttgata ttaatcagtt caaacgcatc aatgcccaat
     1021 ggggacatcg tgtgggtgat aaagtgctgg tttcaattgt cgatattatc cagcaaagca
     1081 tccgccccga tgatatttta gcgcgtctgg agggtgaggt gtttggcttg ctatttaccg
     1141 aactcaatag tgcccaggca aaaatcattg cggaacgtat gcgtaaaaat gtcgaactcc
     1201 tgaccggctt tagtaacaga tatgatgttc ctgaacaaat gaccatcagt attggcacgg
     1261 ttttttcaac gggtgacacg cgtaatatct cgcttgtcat cacggaagca gataaagcct
     1321 tacgcgaagc gaaaagcgag gggggcaaca aagtgattat tcatcatatt taagtgcaaa
     1381 aatattcaga gccatgcttt ttgcgtggct tttgcataca atttattact attaccccta
     1441 aatttcccct ccccagcggc gcagagatga gtataattag cgcccttgtg ccaggccgca
     1501 atcgaacttt atctggtttt ctcgtttcac taaccgaagg agtgccattt atcatgaaat
     1561 tgcaccatag aatgctccgg cattttatcg ccgcaagtgt cattgtgctg acatcttcct
     1621 tccttatttt tgaacttgtc gccagcgaca gagcaatgag tgcctatctg cgctatatcg
     1681 tgcagaaagc agactcctcc tttctttatg ataagtatca gaatcagagt attgccgcgc
     1741 atgtgatgcg cgctctcgct gctgagcagt cggaagtgtc gccagaacag cggcgcgcca
     1801 tctgcgaggc ttttgagtct gccaataata cccatggctt aaacctgact gcccataaat
     1861 acccgggctt acgcggcaca ctacaaaccg catccactga ctgcgacaca attgtggaag
     1921 ctgcagcact attacccgct tttgatcagg cagtggaagg caaccgccac caggatgatt
     1981 acggttcagg tcttgggatg gccgaagaga aatttcacta ttatctcgat ctcaatgacc
     2041 gctatgtcta tttttatgag ccggttaatg ttgaatactt tgcgatgaat aactggtcct
     2101 tcctgcagtc aggaagtatt ggcatcgatc gcaaagatat tgaaaaggta tttaccgggc
     2161 gtaccgtatt gtcgagcatt taccaggatc agcgtactaa acagaacgtg atgagtttgc
     2221 tgacgccggt atatgtcgca gggcagctaa aagggattgt gctgctggat attaacaaaa
     2281 acaatttgcg gaatatcttt tacactcatg accgccctct cctctggcgt tttctcaatg
     2341 tcacgctaac cgataccgat tcggggcgcg acattatcat caaccagagc gaagataatc
     2401 tgttccagta tgtcagttac gtccatgact taccgggcgg cattcgtgtc tcgttatcca
     2461 ttgatattct ttactttatc acgtcttcgt ggaaaagcgt tctgttctgg attttgacgg
     2521 cgttaatttt gctgaatatg gtgcggatgc acttccgctt ataccaaaat gtgtcgcgag
     2581 aaaatattag tgatgcgatg actggactgt ataatcgcaa aattttaacc cctgaactgg
     2641 agcagcggtt gcagaaactg gtgcaatccg gttcttcggt gatgtttatt gcaattgaca
     2701 tggacaagtt aaagctaata aatgacaccc tcggtcatca ggaaggggat ttagcgatta
     2761 cgttgttagc tcaggcgatt aaacaatcga ttcgtaaaag tgattatgcc atccgactcg
     2821 gtggcgatga attctgcatc attcttgtcg attcgacgcc gcaaattgca gcacaactgc
     2881 ctgaacgtat cgaaaaacgt ctgcaacata tcgcgccgca gaaagagatc ggcttctctt
     2941 ccggtattta cgcgatgaaa gaaaacgata cgttacatga tgcgtataaa gcttccgatg
     3001 agcgtttata tgtcaataag cagaacaaaa acagccgttc atgataacct tctgtggttg
     3061 tttgcttgta atctcaggag cgtgaaatga ctgaaatggc taaaggaagc gtgacgcatc
     3121 agcggttaat cgctttatta tcacaagaag gtgctaactt ccgcgtcgtt acccatgaag
     3181 cggtaggtaa atgtgaagcg gtgtcagaaa ttcgtggcac cgctttaggc cagggagcta
     3241 aggcactggt atgtaaagtc aaaggcaatg gcgtaaatca acatgttctg gcgatcctcg
     3301 ctgccgatca acaggccgat ctgagccaac tcgccagcca tataggcgga ttacgcgctt
     3361 cgttggccag cccggcggaa atcgatgaac tcacaggctg tgtcttcggc gcgatccccc
     3421 ctttcagctt ccatccaaaa ctcaaactgg ttgccgaccc tctactcttc gaacgatttg
     3481 atgaaatcgc cttcaatgca gggatgctgg ataaatccgt tattttgaaa accacggatt
     3541 atttgcgcat tgcgcaacca gaactgctca atttccgccg cactgcgtaa ctagcctgtc
     3601 cgttcaataa gcagaacgga caaaaccagc acaacagcaa tggcaaaaaa cgatgacgtg
     3661 ataatcagtg tctcgacgaa catttgatcg ttcatagcat gcccccggga gatagctgtt
     3721 ttcacgttat tgttagcgtg cacaaatggc agtttgatga cagttcgcct gattttttat
     3781 gcaaaaaacg taaagatttt ctacttcctt cctgcagcaa gcgtaaagta agcaggctta
     3841 ttattttttg gcaaggaaac cacgatgttt gatgtcactt tgctgatcct gctcgggtta
     3901 gctgcgctgg gctttatcag tcataacacc actgtcgccg tttcaattct ggtgttgatc
     3961 attgtccgcg tcacaccgtt aagcaccttt ttcccgtgga ttgaaaaaca ggggctaagt
     4021 atcgggataa tcattctgac gattggcgtt atggccccca tcgccagcgg gacgctaccg
     4081 ccctcaacct tgattcactc gtttctgaac tggaaatcgc tggtggcaat tgcagtaggc
     4141 gttattgtct cctggctggg tgggcgcggc gtgaccttaa tgggcagcca gccgcaactg
     4201 gtcgccgggc tgctggtagg cactgtttta ggcgtggcgc tgtttcgcgg cgtaccggtc
     4261 ggcccgctta ttgccgcggg tcttgtttcg ctgattgtgg ggaaacagta gttaatcccg
     4321 caatatatcg gcctggcgtt tgccccaggc ctttcttaaa catagtaata aaggccgttg
     4381 ttgaatcata ccccagcgta tgagcaactt tctgtacagt atcgcctttc actaatccct
     4441 gaagtgccat aatcagctgt aactgctgac gccactggcg aaaactcaac cccgtctctt
     4501 ttactatcag tcgcgcaaga ttgcgttcac tcatcgcaaa aaagccagcc cattgcccta
     4561 atgaccccca ctcgacgggc cctttcgcca tcatctccac catcgtgcgg attttaggat
     4621 gagaagaaac gggcaggtgc agctgttgtt gtggctgctg agggagttca tcaaacagga
     4681 cctgaattaa tctgcgggtc atcggctcag cacgctgagt cgtcgtcctg tttgccaggg
     4741 ttaaaatcaa ttcccggcat aagggcgata ttttcaatgt gcaacatgtt gtcggcattg
     4801 tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc acttgattgc
     4861 tatgttccac cccgcccggt atccaaaccg catattgcgg cggcaccatc cacaaagcat
     4921 tttccaccgt acaggtaatt gcaccatgta atgctagtat cagctgtcct ttgcggtgct
     4981 gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg accgcggctt
     5041 catggtgtcg gtcgggttca tagccattga gattcaacct gtgcatcatt ttgtccgaat
     5101 ttagcgataa tttgtcattt tagcttgatt caacataaca ataaaaacgg taaggtacag
     5161 cctcgtttgt aacaatgaga agcatatgac ctgttcaact tcattaagcg gcaaaaacag
     5221 gattgtcctt atcgctggca ttctgatgat tgccacaaca ttacgcgtca cctttaccgg
     5281 cgcagcgccg ttactggata cgattcgttc cgcttactcg ctgacgacag cgcaaaccgg
     5341 cttattaacc accctgccat tattggcctt tgcgctaatc tcacctttgg ctgccccggt
     5401 ggcgcgacgt tttggtatgg aacgtagcct gtttgccgcg ttacttttga tctgtgcagg
     5461 tatcgcaatt cgttctctcc cttcgcctta cttattattt ggcggtacag cggtcattgg
     5521 cggtgggatt gcattaggca atgtcttact gccaggatta attaaacgcg atttccctca
     5581 ttccgtcgcc agacttaccg gcgcatattc cctgacaatg ggagctgcag cggcactggg
     5641 atcggctatg gtcgtgccgc tggctttgaa cggttttggc tggcaaggcg cgttgttcat
     5701 gctgatgtgt tttcctctgc tggctctttt tttatggctg ccacagtggc gaagccaaca
     5761 acatgcaaat ttgagtacct cgcgcgcctt acatactcgg ggtatctggc gttcgccgct
     5821 tgcctggcag gtcacattgt ttcttgggat caactcactg gtctattacg tgattattgg
     5881 ctggcttccg gcgatcctca tcagtcgcgg ctatagcgaa gcacaggcgg gttcactgca
     5941 tggtttgctg caactagcca cagcagctcc cggtttgctg atccctcttt tcttacatca
     6001 tgtgaaagat cagcgtggta ttgcagcgtt cgttgccttg atgtgcgcag tgggcgctgt
     6061 tgggctctgc tttatgcccg cgcacgcgat cacctggact ctgcttttcg gttttggttc
     6121 cggcgcaaca atgatactgg ggttgacgtt cattggtctt cgggcaagtt ctgcgcatca
     6181 ggcggcggca ctctcgggga tggcacaatc cgtcgggtat ttgctggcag cctgtgggcc
     6241 gccgctgatg ggtaaaatac acgatgctaa cggtgactgg tccgtaccac tgatgggtgt
     6301 tgccatactt tcactactga tggcgatttt cggactttgc gccgggagag acaaagaaat
     6361 tcgctaatat ccggtgctat agtgacgtga caaatcatgc gtgaaaggga gaacaaacac
     6421 gatgaatatt cagtgcaaac gcgtttatga tccggctgaa cagagcgatg gttatcgcgt
     6481 actggtcgac cgcctctggc cgcgcggtat caaaaaaacc gatttagccc ttgatgagtg
     6541 ggataaagaa atcacgccgt caacggaact gcgcaaagcc tttcacggcg aagtcgtcga
     6601 ttttgcaacc tttcgcgagc aatatcttgc agaactggcg caacacgagc aagaaggaaa
     6661 gcggctggcg gacatcgcca aaaaacagcc gctgaccctg ctctacgcag caaaaaacac
     6721 cacgcagaac catgcgctgg tgctggccga ctggctacgt agcttgtgat tttagtacag
     6781 catccggcgg ttatttttca ccagccggat ggtcccgtcg ccacaatgcc cattcatcaa
     6841 tcgtttcacc gcccggtaat ttgcaattgt tgctgacccc ttgcgctgtc tgcactggaa
     6901 tgagcgtccc gcccttctgc tggcaataga ccgacgccgg atttgccata ccaatctgcg
     6961 gcggtttagg tgcttctggc tgagaagagg ttgaacaacc agccaggacc agcaagcaag
     7021 gcaaagcaaa actgataatt ttcatttatt gatctcacac atttatctac gcttagagta
     7081 tcgcggtatc gttttgtttt gcagcactat ttttattaca ttcactcaaa acatattacg
     7141 tcttgtttca tctttgttga tgatgtttta tcatgcctgc aaagattaaa taatcagcat
     7201 ttacccgccg tatcctggag ttgtttcgtg tcagatcaga ttatcgcccg cgtctcgcaa
     7261 tcccttgcca aagaacagtc actggaaagt ctggtccgac agcttctgga gatgctggaa
     7321 atggtcactg atatggaatc aacctacctg accaaagtgg atgtcgaagc gcgcctgcag
     7381 catattatgt ttgcccgtaa cagccagaaa atgcacatcc cggagaattt taccgtctcg
     7441 tgggattact cgttatgcaa acgcgctatt gatgaaaact gctttttcag cgatgaagtc
     7501 cctgaccgtt ggggcgactg tattgcggca cgcaatcttg gaatcaccac atttctgagt
     7561 acaccaattc acttaccgga tggatcattc tatggcacgc tttgcgccgc cagcagtgag
     7621 aagcgccagt ggagtgaacg cgcggaacag gttttacagt tattcgccgg actgattgca
     7681 caatatattc aaaaagaggc gttggttgaa cagctgcgcg aagccaatgc cgcgctgatt
     7741 gcgcaatcgt ataccgactc gttaaccggg ctaccgaatc ggcgggcgat ttttgaaaat
     7801 ctgacgacgc tgttttctct cgcccggcat cttaaccata agataatgat cgcgtttatc
     7861 gatctggata acttcaaatt aatcaatgac cgttttggtc ataatagtgg cgatctgttt
     7921 ctcattcagg ttggcgagcg ccttaatacg ctccagcaaa atggcgaagt tattggtcgt
     7981 ctcggcagtg atgagttttt ggttgtttca ctaaacaacg agaatgcgga tatttcgtcg
     8041 ctgcgagaac gtattcaaca gcaaatacgt ggagaatatc acttaggtga tgttgatttg
     8101 tattatcccg gtgccagtct tggcatagta gaagtcgatc ctgaaacaac cgatgcagac
     8161 agtgccctgc atgctgccga tattgcgatg tatcaggaga aaaaacacaa acagaaaaca
     8221 ccttttgtcg cgcatccagc gctacattcc tgaggcgtat tcacatcctt ttgattggtg
     8281 ataacatgcg aatcggtatt atttttccgg ttgtaatctt tattacagcg gtcgtatttt
     8341 tagcatggtt ttttattggc ggctatgctg ccccgggagc ataaagatga aaaaaacaac
     8401 gattattatg atgggtgtgg cgattattgt cgttctcggc actgagctgg gatggtggta
     8461 acgtcacctc taaaaaatag caaaggctgc ctgtgtgcag cctttgtgca atttaagcgt
     8521 taacttttaa tcttcctgta gataaatagc acgacaatcg caccaataac ggcaaccacg
     8581 aagctgccaa aattgaagcc atcgacttta ccaaagccaa acagtgtgct gatccatccg
     8641 ccgactacgg caccgactat ccccagcaga atagtcataa agaatccacc accatcttta
     8701 cctggcatga tccacttcgc cagaataccg gcaataagcc caaaaataat ccatgacaga
     8761 atgcccattg tttcctcact tatctgtttt gcattagcgg gttagtcgct gataaaaagc
     8821 atagcacaac atcgggaggg caagatttgt gacgagcatc acggaggatt tttttgcgat
     8881 ggcgcagaaa ttgcgccatc aacgatcggt gataattacc aaccacaaac atcatgttcg
     8941 ttttccgtgt cttgacatcc tccacgccct gaatgacgag gatccctgct acgttcaggc
     9001 tgtcgcctga atcatttcgg tgggttcctg cttcaacgag cggcctgact gcaccatctc
     9061 tccacaggca agaacggcgt gccccgccgc taaaatgtta cgagcgccgt ttacatcggc
     9121 gttcgctgta tatccacata cctggcatct gaattgactt tgtgacaggc gattttcttt
     9181 cgctgtatga ccacagcacg cgcaacgctg gcttgtgtac gctggcggaa cagcaagcac
     9241 ctgaccgcca ctccagagct gcttgtactc aagctggcgg cgcatttcat accagccctg
     9301 atccagtatc gaacggttta aacctgattt tgcccggaca ttgcgccccg gctggctgac
     9361 cgtacccgct gctgactttg acatgttgct gaccttcaaa tcctcaatga ctatcattgc
     9421 gtggtttttg ctgacggtcg ttgtgacttt atgaaggtag tccctgcgga tatttgcgat
     9481 acgggaatgc agtcgctgta ttttgcgttt ctgcttctgc cagttattgc tgaatttgac
     9541 cttgcggctt aactgtcgct gaagtctcgc cagcgtcttc tggtttttct gaaaactgtt
     9601 tacaggccca aagactgtgc catctgacag cgtggcgagt ttagccacgc cagcatccag
     9661 cccgatcatt gatgctgaag ggtgaaccgg agttgatact tcactttctg tctgaatact
     9721 gatgtaccac ttaccgcagg actggctgac cgtgacattt ttcacaacac ccgtgacctg
     9781 acggctattc cggtagcgca tccagcccag tttcggcaga aaaatacggc tgttttcctg
     9841 atcgagcttt acgccctgcg ggtagcggaa tgcatcattc tgtccccgct ttttgaatcg
     9901 gggaaaagca gcccgcttcc ggaagaagtt tttgtaagcc cgctcaaggt ctttcagtga
     9961 ctgttgcaat ggctgtgagg gcgaatcttt aagccattgc gtttcagtgg cgtttttcca
    10021 ctcaaccagc caggaagcca ttttcccgta agggatgtat ttattaccgg cctcatgatt
    10081 ctcattctga cgtgccagag cacgattgaa aacgaaacga catgcgcctg cgaagcgcct
    10141 catttcacac tcctgttgac cacctggtct taactggaat ttaaatgcct gtaatcgttt
    10201 cacaggtcaa atgatacttt ggtctgtgaa aaaagaaacc gatatccggc gtggaaggca
    10261 ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc agataccgac tccagatttt
    10321 tgaccatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg ctgattttga
    10381 agctgaactt gtt
//
LOCUS       DAZGAN020000152        10124 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGAN020000152 DAZGAN020000000
VERSION     DAZGAN020000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.152"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            172..675
                     /gene="ftnB"
                     /locus_tag="ACHKYO_RS22145"
                     /old_locus_tag="pgaptmp_004429"
     CDS             172..675
                     /gene="ftnB"
                     /locus_tag="ACHKYO_RS22145"
                     /old_locus_tag="pgaptmp_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HGU4431163.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(754..1005)
                     /gene="yecJ"
                     /locus_tag="ACHKYO_RS22150"
                     /old_locus_tag="pgaptmp_004430"
     CDS             complement(754..1005)
                     /gene="yecJ"
                     /locus_tag="ACHKYO_RS22150"
                     /old_locus_tag="pgaptmp_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HGU4431164.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1120..1206)
                     /gene="azuC"
                     /locus_tag="ACHKYO_RS22155"
                     /old_locus_tag="pgaptmp_004431"
     CDS             complement(1120..1206)
                     /gene="azuC"
                     /locus_tag="ACHKYO_RS22155"
                     /old_locus_tag="pgaptmp_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HGU4431165.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1469..1792
                     /gene="yecR"
                     /locus_tag="ACHKYO_RS22160"
                     /old_locus_tag="pgaptmp_004432"
     CDS             1469..1792
                     /gene="yecR"
                     /locus_tag="ACHKYO_RS22160"
                     /old_locus_tag="pgaptmp_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HGU4431166.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1963..2460
                     /gene="ftnA"
                     /locus_tag="ACHKYO_RS22165"
                     /old_locus_tag="pgaptmp_004433"
     CDS             1963..2460
                     /gene="ftnA"
                     /locus_tag="ACHKYO_RS22165"
                     /old_locus_tag="pgaptmp_004433"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HGU4431167.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2498..2737)
                     /gene="yecH"
                     /locus_tag="ACHKYO_RS22170"
                     /old_locus_tag="pgaptmp_004434"
     CDS             complement(2498..2737)
                     /gene="yecH"
                     /locus_tag="ACHKYO_RS22170"
                     /old_locus_tag="pgaptmp_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HGU4431168.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            2928..4139
                     /gene="tyrP"
                     /locus_tag="ACHKYO_RS22175"
                     /old_locus_tag="pgaptmp_004435"
     CDS             2928..4139
                     /gene="tyrP"
                     /locus_tag="ACHKYO_RS22175"
                     /old_locus_tag="pgaptmp_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HGU4431169.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4201..4866)
                     /gene="yecA"
                     /locus_tag="ACHKYO_RS22180"
                     /old_locus_tag="pgaptmp_004436"
     CDS             complement(4201..4866)
                     /gene="yecA"
                     /locus_tag="ACHKYO_RS22180"
                     /old_locus_tag="pgaptmp_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HGU4431170.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKLGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5062..5148)
                     /locus_tag="ACHKYO_RS22185"
                     /old_locus_tag="pgaptmp_004437"
     tRNA            complement(5062..5148)
                     /locus_tag="ACHKYO_RS22185"
                     /old_locus_tag="pgaptmp_004437"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5112..5114),aa:Leu,seq:taa)
     gene            complement(5161..5234)
                     /locus_tag="ACHKYO_RS22190"
                     /old_locus_tag="pgaptmp_004438"
     tRNA            complement(5161..5234)
                     /locus_tag="ACHKYO_RS22190"
                     /old_locus_tag="pgaptmp_004438"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5200..5202),aa:Cys,seq:gca)
     gene            complement(5288..5363)
                     /locus_tag="ACHKYO_RS22195"
                     /old_locus_tag="pgaptmp_004439"
     tRNA            complement(5288..5363)
                     /locus_tag="ACHKYO_RS22195"
                     /old_locus_tag="pgaptmp_004439"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5328..5330),aa:Gly,seq:gcc)
     gene            complement(5515..6063)
                     /gene="pgsA"
                     /locus_tag="ACHKYO_RS22200"
                     /old_locus_tag="pgaptmp_004440"
     CDS             complement(5515..6063)
                     /gene="pgsA"
                     /locus_tag="ACHKYO_RS22200"
                     /old_locus_tag="pgaptmp_004440"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGU4431171.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLEQ"
     gene            complement(6120..7952)
                     /gene="uvrC"
                     /locus_tag="ACHKYO_RS22205"
                     /old_locus_tag="pgaptmp_004441"
     CDS             complement(6120..7952)
                     /gene="uvrC"
                     /locus_tag="ACHKYO_RS22205"
                     /old_locus_tag="pgaptmp_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGU4431172.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7949..8605)
                     /gene="uvrY"
                     /locus_tag="ACHKYO_RS22210"
                     /old_locus_tag="pgaptmp_004442"
     CDS             complement(7949..8605)
                     /gene="uvrY"
                     /locus_tag="ACHKYO_RS22210"
                     /old_locus_tag="pgaptmp_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HGU4431173.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8747..8818
                     /locus_tag="ACHKYO_RS22215"
                     /old_locus_tag="pgaptmp_004443"
                     /pseudo
     CDS             8747..8818
                     /locus_tag="ACHKYO_RS22215"
                     /old_locus_tag="pgaptmp_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            8901..9077
                     /gene="yecU"
                     /locus_tag="ACHKYO_RS22220"
                     /old_locus_tag="pgaptmp_004444"
     CDS             8901..9077
                     /gene="yecU"
                     /locus_tag="ACHKYO_RS22220"
                     /old_locus_tag="pgaptmp_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HGU4431174.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9064..9288
                     /gene="yecF"
                     /locus_tag="ACHKYO_RS22225"
                     /old_locus_tag="pgaptmp_004445"
     CDS             9064..9288
                     /gene="yecF"
                     /locus_tag="ACHKYO_RS22225"
                     /old_locus_tag="pgaptmp_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HGU4431175.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9355..10077)
                     /gene="sdiA"
                     /locus_tag="ACHKYO_RS22230"
                     /old_locus_tag="pgaptmp_004446"
     CDS             complement(9355..10077)
                     /gene="sdiA"
                     /locus_tag="ACHKYO_RS22230"
                     /old_locus_tag="pgaptmp_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HGU4431176.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2544 a         2546 c         2331 g         2703 t
ORIGIN      
        1 tttaagcatt ttcttatacc cgttcagacg ttattcttat ttcagatcat cgtcagaatt
       61 gactccacga tcacatttcg gaccggcaga aaggaattat tctgcaaaca gtaattatgg
      121 tgttttgatt tatcttgcac ctctccactt ctggatataa ggatattagg tatggcaacc
      181 gctggaatgc ttctcaaact caactctcaa atgaaccgcg agttttacgc atccaatctc
      241 taccttcacc tgagtaactg gtgttctgaa cagagtctga acggcaccgc cactttcctt
      301 cgcgcccagg cacagagtaa tgtgacccaa atgatgcgca tgtttaactt tatgaagagt
      361 gtcggcgcta cccccatcgt taaagccatt gatgttcccg gtgaaaaact gaactctctg
      421 gaagaactgt tccaaaaaac gatggaagaa tacgagcaac gttctagtac gttggcacag
      481 ttagccgatg aagcgaaaga actgaatgat gattcaaccg tcaatttcct gcgcgatctg
      541 gaaaaagaac agcagcatga tggtctgttg ctgcaaacca ttcttgatga agtgcgcagt
      601 gcgaaacttg cgggtatgtg ccctgtgcag accgaccaac atgttctgaa tgtcgtgtca
      661 caccagctgc attgatcatc atcggcgcta atgcattgcg ccgatgaagg ttttgagaaa
      721 ccgctgcctc atctgtttga agcagcggtt tttttaatgg gattcaccct gtggggtaaa
      781 cttgagttca ataagcgcga tggctttttg gattgcccgc atggtgaccg ggtctgcggc
      841 ggcgggatgg ttagtaaagt cgatattctt cagctgactg gacatttttt cacgaacttc
      901 aacgggcgcg attacatcga gaacatccag aatttgtttg ataaccaact ggcaagcaac
      961 cacatcagaa accagttcct gatcggcatt cagcggctgg gacatcgtaa actcctgata
     1021 gcattttgaa agccgttata gtagcggctt cacatcttca gcgatagtca catccaccgt
     1081 catcaggaca caaaaaaacc tgccggagca ggttttttgt tatcggaaca tattgcctgg
     1141 cggtacgtct ttgaacgtct tgcaatagtt attgaacata cttttcagga ttttgcgcag
     1201 tttcatcgcg gcactccgac catttgttat acaggtgtta ttgtctttgc gcttataata
     1261 tgacaaccat cacaaaaatc aatctttatg tgatacaaat cacataaata cccctttaat
     1321 gttataaaaa tgataatcaa aaaacagccc ccctatttct gacacctaca gatggcaaga
     1381 aatagcgcct gccaggcgtc ttttccggcc attgtcgcag cactgtaacg cgtaaaatag
     1441 tgctttctct tactcttctg gctggaccat gagacttctg attctgactc tttcattaat
     1501 aacgctggcg gggtgtacgg ttactcgcca agcccatgtg agcgaagttg acgcggcaac
     1561 aggtattgta cggttagttt atgaccaggc ttttttgcag catgcccata ccgatcgtta
     1621 tgtcagtcgc gggatcgccg atcgcgcatg ccagcaggaa ggctataccc acgcggtccc
     1681 ctttggtcag ccagtaggca actgtagcct ttttgcgggt tccttatgtt tgaatacgga
     1741 attcactttg tcatatcagt gccatcattc cgccttccct gtctttctct aaatatttta
     1801 atcagcgagg ggatcttcgc tgattaaaga aatagcggta atgcgtttta ttcccattcg
     1861 tatttttaat aattgaagtt tatattttac cttttgcaaa taataaaata acaaattata
     1921 gtgacgccac agcaacaaat ataacctttg tggagcacta tcatgctgaa accagaaatg
     1981 attgaaaaac ttaatgagca gatgaacctg gaactgtact cttcactgct ttatcagcaa
     2041 atgagcgcct ggtgcagcta tcataccttc gaaggtgctg ccgcgttcct gcgccgtcac
     2101 gcccaggaag agatgacgca tatgcagcgt ctgtttgatt acctgactga taccggcaat
     2161 ttaccgcgta ttaataccgt tgaatctccg tttgctgaat attcctcact tgatgaatta
     2221 ttccaggaaa cctataaaca cgaacaatta atcacccaga aaattaacga actggctcat
     2281 gctgcaatga ccaatcagga ctacccaaca tttaatttcc tgcaatggta tgtttctgag
     2341 cagcatgaag aagagaaact gttcaaatcg attattgata aattaagcct ggcaggcaaa
     2401 agcggcgaag gtctgtattt tatcgacaaa gaactctcta ccctcgacac acaaaactaa
     2461 tgctctccgg cggcaggttt actgccgccg tttcaaatca gtgacggcaa atcttactct
     2521 gatcggtcga aaaaccgtct ttcgaaggta taaatttgcc ttttgctgcc agaaacgcta
     2581 ccagctctcc cgctgtcatc ccttctgccg agcaggtgtg aaaacgtgcc tgttcaccaa
     2641 aacgcgcttt aatcgcagct tccagactgg catgcgtata ttgctcgcct gattcaatca
     2701 tcatatttaa cacttcatga ccgtgaatag agtccatcgt ccctcctcaa aaaaagccta
     2761 gcgtagcgat tgccgcttat gaagactttg cgccagcgca ggactgaatg ctttttattg
     2821 tacatttata tttacaccat atgtaacgtc ggtttgacga agcagccgtt atgccttaac
     2881 ctgcgccgca gatatcactc ataaagatcg tcaggacaga agaaagcgtg aaaaacagaa
     2941 ccctgggaag tgtttttatc gtggcgggaa ccacaattgg cgcaggcatg ctggcaatgc
     3001 cgctggctgc ggccggtgtt ggttttagcg ttacgttaat cttgttgatt gggctttggg
     3061 cgttgatgtg ctacacggcg ctattactgc tggaggtgta ccagcatgtt ccggcagata
     3121 ccggtctggg cacgctggca aaacgctatc tgggacgcta cggtcaatgg ctgacgggct
     3181 tcagtatgat gttcttaatg tatgctctga ctgcggcata catcagcggt gccggtgaat
     3241 tgttggcctc cagcatcagc gactggacag gtatttcaat gtcggcaacc gctggcgtgc
     3301 tgttgttcac ttttgttgcc ggtggcgtgg tttgtgtcgg aacttcactg gtcgatttat
     3361 ttaaccgttt tctgttcagc gccaagatta tttttctggt ggtaatgctg gtactactgc
     3421 tgccgcatat tcacaaagtg aatcttttaa ccctgccgtt gcaacagggg ctggctctgt
     3481 ctgcaatccc ggtgattttt acctcatttg gttttcacgg tagcgtgccg agtattgtca
     3541 gctatatgga tggcaacgtt cgtaagctac gctgggtgtt tataaccggt agtgcgatcc
     3601 ccctggtggc atatattttc tggcaggtgg caacgcttgg cagcattgat tcaacaacct
     3661 ttatgggatt gctggctaat catgccggat taaacgggct gttacaggca ttacgcgaaa
     3721 tggtggcctc tccgcatgtt gagctggcag tgcatttatt tgctgattta gccctcgcca
     3781 cgtcatttct cggcgttgcg ttaggcttat ttgattatct ggctgattta tttcagcgtt
     3841 caaataccgt tggtggacgg ttgcaaactg gtgcaattac ctttctgccg ccgttggcgt
     3901 ttgcactgtt ttatccacga ggatttgtga tggcgctggg ttacgccggt gtggcgctgg
     3961 cggtactggc attgattatc ccttcgctgt tgacctggca aagcagaaag cacaatcctc
     4021 aggcgggtta ccgggtgaaa ggtggtcgtc cggcgctggt ggtggtgttt ctctgtggta
     4081 ttgctgtgat tggcgtgcaa tttttgattg cggcagggtt gttaccagaa gtggggtgat
     4141 cagatagcct caaattcctt attgggtgcc agaattaacg ctgacaccca atttgacctc
     4201 ttaatgcagg cagcactgct taaatttctt accactaccg cacgggcaag gatcgttacg
     4261 ccccagtttc tcttctgctt tgatcggttg ctgaacagct ttttcctgcg gatgcgccat
     4321 ccagtacgca tgtagatcaa gcgccgccag tcgaatggca tctacgctct cttcaaacgc
     4381 ttctggcgac attttctcta cccgctcgaa gttttcctca gtaccgtgca gcgcaatcgc
     4441 ctccagcgct ggttttaacg aatcgggcaa cgttgaccag tcagaaagtg ccacgccccg
     4501 catatagcca aagcaccact cctcaacaat cgtcagctcg ctgccatcaa cttctcgcaa
     4561 gccgaataac ggctcaaact gctccgggaa ttcgttcaga cgctctgcgg tatcggccat
     4621 atgttgaaaa gccagattca taaagcgcgt catctctttc tctgacgccc agcgcggcac
     4681 atagtcagcc ccaccccaca cggcaaccag ccactgttcc ggttcaatct cttgcggaga
     4741 actcaacacc gccgtcaata aaccgtccag ctccgccaca tcaaggatgg cgtggtcagt
     4801 gttgtatttg gtcagaatat cgtccagcca ttccaactca ctttcgttta acggtcccgt
     4861 tttcatacgc ttttccttgt ggatctcaac tcgccagcac ctatcttaca tgccggtccg
     4921 tatcagagat actttttgag tggctttgct ggtgattaaa aattaaggag ggtgtaacga
     4981 caagttgcag gcacaaaaaa accacccgaa ggtggtttca cgacactgct tattgctttg
     5041 attttattct tatctttccc atggtacccg gagcgggact tgaacccgca cagcgcgaac
     5101 gccgagggat tttaaatccc ttgtgtctac cgattccacc atccgggctc gggaagaaag
     5161 tggaggcgcg ttccggagtc gaaccggact agacggattt gcaatccgct acataaccgc
     5221 tttgttaacg cgccaaattc ttcaggcctt tcagccagac atccgctgac gccgatgtct
     5281 tttaaactgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc
     5341 tctaccaact gagctattcc cgcattcatc aagcaatcag ttaatcactt gattttatta
     5401 tcgtctggca atcagtgccg ccgttcgatg cgttgcattc tacttacctg gcgcgatgag
     5461 tcaacgatat ttttcaccac ttttgattgt ttgctgaaaa ttacgccgaa acgatcactg
     5521 ttcaagcaaa tctgcacgcg cagcgctcaa atattgcaac attgaccaca gagtcagtac
     5581 cgcagccaca aagaaaagtg caataccggc gtactcaacc caaatgttcg gacgccacag
     5641 cagccaggcc aacgccacca tctgggcagt ggttttcact ttcccaatcc aggagacagc
     5701 cacgctactg cgtttaccca actccgccat ccattcgcgt agcgcagaaa taataatttc
     5761 acgggcgatc atcgttgccg ccggtaaggt cacccaccag ctgtgataat gctcggttac
     5821 cagcaccatg gcgatagcca cgagaacttt atctgcgaca gggtcaagga aagcaccaaa
     5881 ccgggtactc tggttccagc ggcgtgccag aaaaccatcg aaccagtcag tcaccgccgc
     5941 gacgcagaaa atgagcgcgg cggcaaacgg cgaccaggtg acaggcagat aaaagaccaa
     6001 tacaaagaat gggataagga tgacacggaa cagtgtaagc aacgtaggga tattaaattg
     6061 cataatgacg ggtaactatc tgttgtcagt aagattaccc ctatgttgct acagagacat
     6121 caatgtttca acgaccagaa gatcttttct gccagacctt gcgaaatacc cggcactttt
     6181 gcaatttcct cgacgctggc gttacgtaaa ccttgcaaac cgcccatata tttcaacaac
     6241 atttgccggc gttttggccc gacgccttca atggtttcca gggaactggt atttttgacc
     6301 ttcgcccgtt ttttacggtg cccgccaatc gcgtgatcat gtgattcatc gcgaatatgc
     6361 tggataacat gcagcgcggg agaatctggc ggcaaactga atccctcacc ttccggctca
     6421 aagaacagcg tttccagccc agccttacga tctgctcctt tggcaacgcc aagtagcagc
     6481 ggatgatttt tatcccatga gacatccagt tcggcgaaga catttttcgc ctgcgcaagc
     6541 tggcctttgc cgccgtcgat aagtatcaca tccgggatct tactgtcgtc gatggcttta
     6601 ccataacgcc gacgcagcac ctgattcatc gccgcataat catcgcccgg cgtgatgcca
     6661 gtaatgttat agcgccgata ctccgcacgc agcgggccgt tagcatcaaa caccacacag
     6721 gaagcgacgg tttgttcgcc catggtatgg ctgatgtcaa agcactccat ccgcttcact
     6781 tccggcaatt tcaacacgct ggcaagcgcg gtcagtcgct ggtgaacggt agattgctgc
     6841 gaaagtttgc tggttaaggc cgtcgccgca ttggtgcgcg cgagtttcag ataacgcgcc
     6901 ctatcgccgc gaggtttggt ttgaacatta atcttgcgtc ccgccagttc tgaaagggaa
     6961 tcggcgagca gcgttttatc gctaagatta aaatcgagca ggatctcacc cggtaaggtg
     7021 cgcatctggc tgccttgtaa atagaactgg cctacgaagg tttctaccac ctcgctcagt
     7081 tccgtaccgc caggcacttt cgggaaatag ctgcggctgc cgagcacttt gccctgacga
     7141 atgaacaata cgtggacaca agccatgccc gcatcgaacg ccacaccaat aacgtcgagg
     7201 tcgtcgccgg tattggaaac aaactgtttt tcggtgacgc gtcgcaccgc ctgaatttgg
     7261 tcgcgaatac gtgcagcttc ttcaaactcc agattctggc tggcagtttc catacgacta
     7321 atgagttgcg taagcacctg atcatctttg ccagacaaaa acaggcgcac atactcgacc
     7381 tgctgagcgt attcttcttc actcaccagt ccttcaacgc acggtcccag acagcgccct
     7441 atctggtatt gcagacacgg acgcgagcga ttgcgataaa cactattttc gcactggcga
     7501 atggggaaaa tcttttgtag tagcgccagt gtttcacgta cggcatagcc attcgggaac
     7561 gggccgaaat attcaccttt ggcatgcttc gcaccacgat gcatcgccag acgcgggtgg
     7621 gtatcaccac tcaggaagat aaaaggatat gatttatcat cgcgtagcaa aacgttgtaa
     7681 cgcggctgat agagtttgat gtagttgtgt tccagcaaca gcgcttcggt ttctgtgtga
     7741 gtaaccgtta catcaatttg ctggatctgg gcgaccagcg cttcggtttt gcgcgaagcg
     7801 aggttgctac ggaaatagct ggaaagccgt tttttcaggt ctttcgcttt gccgacatag
     7861 ataaccgtac caccagcatc gtacatgcga taaacgcctg gctggctggt tacggttttt
     7921 aaaaacgctt ttgcgtcaaa ctgatcactc actgacttga taatgtctcc gcattacaca
     7981 gaccatggcg aattgccagg tgagtcagct caacatcgcc atgaatgttt agtttactga
     8041 acatacgata gcggtagctg ttcaccgttt tcggactgag attgagctgt tctgagatct
     8101 cattgacctt ctggcccttg gtgatcatca gcataatctg caattcacgt tcagacaaac
     8161 tggcaaatgg gctttctgtt ttttctggtt cgatctggct taacgccatt tgttgagcga
     8221 tgtcagaagc aatgtaacgc tgccctgaat agacagaacg aatcgcactc acgacttcct
     8281 gcggagccgc gcctttgctg aggtagcccg cagcaccggc ctgcatgact ttcgctggta
     8341 aagggttttc tgtatggacg gtaagcatga tgattttgac atcagctgtg gaacgcgcga
     8401 ttttacgcgt cgcctcaaga ccgccaatgc ccggcatact catgtccatt agcaccacgt
     8461 caacggcatt tgtccggcac cacttaacgg cgtcttcacc gcacgatgcc tcaccgacga
     8521 cttttatacc ctttatatct tccagaatgc gtcgtatccc tgcgcgcacc agttcgtggt
     8581 catcaacaag tagaacgttg atcaaaggaa tatctccaga aatagggata acgctactga
     8641 tagttagtca ttcgtatatt agcggttttt attgcaactt tgaaacgtta aaaatggtgg
     8701 gtattcgatt tttctctcgt ttttgtcaat tcgattgtcc acagttatgg tcacgcccgt
     8761 caccaacggc ttaccggatc gtttcacagt aagccctacg atgaaatgtg acaaaaattg
     8821 actttattca gcaaaaatga aaatcagccc gctgaatatg taacattaat taacccgaac
     8881 aatacgggca aaaaacattc atgataaaaa tatttattgg tcattatatt aacgtatttt
     8941 atagcactgc cgatatcacg ctcaaaaaac aaccactgct atttttagca aagcttatgg
     9001 tatactccgc cgccttaaca tttttcaccg caaattttca ttgcaacatg acgaggaaaa
     9061 taaatgagta cgcctgattt ttctactgcc gagaataatc aagaactggc aaatgaagtc
     9121 tcctgcctga aagcgatgct gacgctgatg ctgcaggcga tgggacaagc tgacgcgggc
     9181 cgcgtgatgt taaagatgga aaaacagctt gcgctgatcg aagacgaaac ccaggctgca
     9241 gtattttcca aaacggttaa gcaaattaaa caagcctacc gtcagtaata taaaaccggc
     9301 tgagagcgtg cctttcagcc ggtttttgca tctggcacgc agaaagaaaa gcgatcaaat
     9361 taagccagta gcggccgcgt aacaggccac ctgggtctta tttggtgcat tgattttttt
     9421 ctgcatgttt ttctgatgga aattgaccgt attctcagag attgacaaaa tcatcgctat
     9481 ctctgctgat gtcttccctt ccgccgtcca tttcagaatt tctttttcgc gcttgctgaa
     9541 attcatctct ggcgtcatca ctatttcatc atttaaacgc atcagagcca tcagactttc
     9601 gcgcaccagt aactgcattt ttaattgcag ttcatcacta agaatgggta tttcgcgcgt
     9661 gctgcaacgg gaaaaggaca aaaagcccag cgcccgattt ggcagcatta aatactgagt
     9721 gacaccgcgg cgtaaaccat gtgcgcgcgc ggcttcccat aacggctgtg cttcgctgaa
     9781 taagtcatca ttccacatta aatggccctg actaaagttt tcagggttca gcaccggatc
     9841 aattgcgaga aagttttttg cctgataata actaacccac gcctcagggt aattggtgta
     9901 gaaagccact ttaggtcgcg tgaatggtac cgggtggcga acacataacg aatagtaatc
     9961 gtactccagc tgctgagcct gaagctctat ttcatggtag acctcttctg cggcctccat
    10021 cctctgaaaa cgcaacagca tcgtgcgacg ccagctgaaa aaatccgtat cctgcatagt
    10081 aaaccgcaac gcccctgaga gtgagtatca tttataatga taat
//
LOCUS       DAZGAN020000153        10088 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGAN020000153 DAZGAN020000000
VERSION     DAZGAN020000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.153"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1603
                     /gene="speC"
                     /locus_tag="ACHKYO_RS22235"
                     /old_locus_tag="pgaptmp_004447"
     CDS             <1..1603
                     /gene="speC"
                     /locus_tag="ACHKYO_RS22235"
                     /old_locus_tag="pgaptmp_004447"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HGU4431177.1"
                     /translation="SSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGATPVYLE
                     TARNPYGFIGGIDAHCFEESYLRELITEVAPQRAKEARPFRLAVIQLGTYDGTIYNAR
                     QVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQAG
                     FSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYPLFAALDINAKMHEGVSG
                     RNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPG
                     EHWHSFEGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATILANFLRENGVVPEK
                     CDLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLAEVLPSIYKQHEERYAGYT
                     LRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAYLRGEVELVRLPDAE
                     GRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHD
                     GRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            1600..2919
                     /gene="potE"
                     /locus_tag="ACHKYO_RS22240"
                     /old_locus_tag="pgaptmp_004448"
     CDS             1600..2919
                     /gene="potE"
                     /locus_tag="ACHKYO_RS22240"
                     /old_locus_tag="pgaptmp_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HGU4431178.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(2975..4615)
                     /gene="pgm"
                     /locus_tag="ACHKYO_RS22245"
                     /old_locus_tag="pgaptmp_004449"
     CDS             complement(2975..4615)
                     /gene="pgm"
                     /locus_tag="ACHKYO_RS22245"
                     /old_locus_tag="pgaptmp_004449"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HGU4431179.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(4641..5186)
                     /gene="seqA"
                     /locus_tag="ACHKYO_RS22250"
                     /old_locus_tag="pgaptmp_004450"
     CDS             complement(4641..5186)
                     /gene="seqA"
                     /locus_tag="ACHKYO_RS22250"
                     /old_locus_tag="pgaptmp_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HGU4431180.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5371..6135
                     /gene="ybfF"
                     /locus_tag="ACHKYO_RS22255"
                     /old_locus_tag="pgaptmp_004451"
     CDS             5371..6135
                     /gene="ybfF"
                     /locus_tag="ACHKYO_RS22255"
                     /old_locus_tag="pgaptmp_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4431181.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6275..6568
                     /gene="ybfE"
                     /locus_tag="ACHKYO_RS22260"
                     /old_locus_tag="pgaptmp_004452"
     CDS             6275..6568
                     /gene="ybfE"
                     /locus_tag="ACHKYO_RS22260"
                     /old_locus_tag="pgaptmp_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HGU4431182.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            6708..7238
                     /gene="fldA"
                     /locus_tag="ACHKYO_RS22265"
                     /old_locus_tag="pgaptmp_004453"
     CDS             6708..7238
                     /gene="fldA"
                     /locus_tag="ACHKYO_RS22265"
                     /old_locus_tag="pgaptmp_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HGU4431183.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7448..7534
                     /gene="uof"
                     /locus_tag="ACHKYO_RS22270"
                     /old_locus_tag="pgaptmp_004454"
     CDS             7448..7534
                     /gene="uof"
                     /locus_tag="ACHKYO_RS22270"
                     /old_locus_tag="pgaptmp_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HGU4431184.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7527..7973
                     /gene="fur"
                     /locus_tag="ACHKYO_RS22275"
                     /old_locus_tag="pgaptmp_004455"
     CDS             7527..7973
                     /gene="fur"
                     /locus_tag="ACHKYO_RS22275"
                     /old_locus_tag="pgaptmp_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HGU4431185.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8057..8383)
                     /gene="chiQ"
                     /locus_tag="ACHKYO_RS22280"
                     /old_locus_tag="pgaptmp_004456"
     CDS             complement(8057..8383)
                     /gene="chiQ"
                     /locus_tag="ACHKYO_RS22280"
                     /old_locus_tag="pgaptmp_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HGU4431186.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8433..9839)
                     /gene="chiP"
                     /locus_tag="ACHKYO_RS22285"
                     /old_locus_tag="pgaptmp_004457"
     CDS             complement(8433..9839)
                     /gene="chiP"
                     /locus_tag="ACHKYO_RS22285"
                     /old_locus_tag="pgaptmp_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HGU4431187.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDSTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPNAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2410 a         2515 c         2559 g         2604 t
ORIGIN      
        1 ttcatcttct aataaagtgg tcttaaacgc attgctgacg ccgggcgatc tggtgttgtt
       61 tgaccgcaat aaccataaat ctaaccacca cggtgcgttg ctacaggctg gcgcgacacc
      121 ggtttatctg gaaaccgcac gtaacccgta tggttttatc ggtggtattg acgctcactg
      181 ttttgaagaa agttacctgc gtgagctgat tacagaagtt gcaccgcagc gggcaaaaga
      241 ggctcgtcct ttccgcctcg ccgttattca gctaggcact tacgacggta cgatttataa
      301 cgcccgtcag gtggtggata aaattggtca tctgtgtgac tacattctgt ttgactccgc
      361 atgggttggc tatgagcaat ttatcccgat gatggctgac tgttcgccgc tgttgctgga
      421 tcttaatgag aacgatccgg gtattctggt tacgcaatcc gtacataaac agcaggctgg
      481 tttttctcaa acttcacaaa tccataaaaa agacagccac atcaaagggc aacagcgtta
      541 tgtaccgcac aaacgcatga acaacgcctt tatgatgcac gcctccacca gcccgttcta
      601 tccgctgttt gccgcactgg atatcaacgc caaaatgcat gaaggtgtca gcggtcgtaa
      661 tatgtggatg gattgtgtgg taaatggcat taatgcccgc aaactgatcc tcgataactg
      721 tcagcatatt cgtccgttcg tacctgaact ggtggatggt aaaccctggc agtcgtatga
      781 aacagcgcaa attgcggttg atctgcgctt cttccagttt gtaccagggg aacactggca
      841 ttcttttgaa ggctatgcag agaatcaata ctttgtcgat ccttgcaaac tgttgctgac
      901 aaccccaggt attgatgcac gtaacggcga atatgaagcg ttcggtgtac ccgcgacgat
      961 tcttgctaac ttcctgcgcg aaaatggcgt agtgccggaa aaatgcgatc ttaactccat
     1021 cctcttcctg ctgactccgg cagaagatat ggccaagctt cagcaacttg ttgccctgct
     1081 ggtacgcttc gaaaaactgc ttgagtccga cgcgccatta gcagaagtgc tgccttccat
     1141 ctacaaacag catgaagagc gctacgccgg ttataccctg cgtcagttgt gtcaagaaat
     1201 gcatgatttg tatgcccgtc acaacgtgaa acaactgcaa aaagagatgt tccgtaagga
     1261 acacttccca cgcgtaagca tgaatccgca agaagccaac tacgcctatt tacgcggtga
     1321 agtggagctg gttcgtctgc cggatgcaga aggccgtatc gctgccgaag gtgcgcttcc
     1381 ttatcctccg ggtgtgctgt gcgttgttcc gggtgaaatc tggggtggtg ctgttctgcg
     1441 ttacttcagc gctctggaag aagggatcaa cctgttgcca ggttttgcac cggagcttca
     1501 gggtgtctat atcgaagaac atgatggtcg taagcaagtt tggtgctatg tcatcaagcc
     1561 tcgtgatgcg caaagtaccc tgttgaaagg ggaaaaatta tgagtcaggc taaatcgaac
     1621 aaaatgggcg tcgttcagtt aaccatactg acgatggtca acatgatggg ctccggtatc
     1681 atcatgctgc cgacaaagct tgccgaagtt gggacaattt caattatctc ctggctggtg
     1741 acagccgtag gctcaatggc actggcatgg gcattcgcaa aatgcggtat gttcagccgt
     1801 aaatcaggtg gtatgggcgg ttatgccgag tatgctttcg gtaagtccgg caattttatg
     1861 gcgaactaca cctacggtgt ctcgctgctg attgctaacg tcgcgattgc tatttcggcg
     1921 gttggttacg gcaccgaact gctcggcgcg agtttgtcgc cagtgcagat tggtcttgcg
     1981 accatcggag tgctgtggat ttgtaccgtg gctaactttg gtggtgcgcg cattaccggg
     2041 caaatcagta gcattactgt gtggggggtc attattccgg tggtaggcct gtgcattatc
     2101 ggctggttct ggtttagccc gacgctttac gttgattcct ggaatccgca tcatgcaccg
     2161 ttcttcagtg cagtaggttc ttccatcgct atgacgctgt gggcttttct cggtctggag
     2221 tctgcgtgtg cgaatactga tgtagtggaa aacccggaac gtaatgtgcc aatcgcggta
     2281 ctcggcggta cgttaggtgc ggcggtgatt tatatcgtct ccaccaacgt gattgcaggg
     2341 attgtgccta atatggagct ggcaaattca acggcaccat ttggcctggc cttcgcacag
     2401 atgttcacac cagaagtggg taaagtcatt atggcgctga tggtgatgtc ctgctgcggt
     2461 tcgctgcttg gctggcagtt caccattgcc caggtgttta aatcttcatc tgatgaaggc
     2521 tacttcccta aaattttctc ccgtgtaacc aaagtggatg caccggtgca gggaatgttg
     2581 accattgtga ttatccagag cgggttggcg ctgatgacga ttagcccgtc gctaaacagt
     2641 cagttcaacg tgctggttaa cctggccgtg gtgaccaata tcattccgta tattctgtca
     2701 atggcggcat tagtcattat tcagaaggtc gctaatgtgc ccccctcaaa agcgaaagtt
     2761 gcaaactttg ttgcttttgt tggcgcgatg tatagctttt atgcgctgta ctcatccggg
     2821 gaagaagcca tgctgtacgg ttccatcgtg accttccttg gttggacact gtatggtctg
     2881 gtatcaccac gctttgaact gaaaaataaa cacggttaaa aaagcaaagt aaaaaagggc
     2941 gatcttgcga ccgccctttt tttattaaat gtgtttacgc gtttttcaga acttcgctaa
     3001 caatctcaac cgcttctttc tcaatctgct tgcgatgttc ttcaccgagg aagctttcgc
     3061 agtagatctt atatgcgtct tccgtgcctg acggacgcgc ggcgaaccag ccgttgtcgg
     3121 tcatcacttt cagaccgccg atagaagcac cattgcccgg tgccgccgtc aggcgcgcgg
     3181 tgatcgggtc gcctgcaagg gtgctggcgc tcaccatttc cggagacagc ttagacagcg
     3241 ccgctttttg tgcggaagtc gcggctgcct gcaaacggtt gtagctcggc gcaccaaagc
     3301 gttttgccag ttcgttgtag tgttcctgcg ggttcttacc ggtgacagcg gtgatttccg
     3361 ccgccagcag acacataatg atgccgtctt tgtcggtgga ccacggcgtg ccgtcgaaac
     3421 gcaggaagga agcccctgcg ctctcttcgc cgccaaagcc gaagctgccg tcgaacagac
     3481 catcaacaaa ccatttgaaa cctaccggga cttctaccag cttacggccc aagtcattga
     3541 ccacacggtc gatcatcgca gaagaaacca gcgttttacc gacagcaaca tctttgcccc
     3601 actgcggacg atgctggaac aggtagttga tcgccaccgc caggtagtgg ttcggattca
     3661 tcaaacctgc cggagtgacg ataccgtgac ggtcataatc cgggtcgtta gcaaacgcca
     3721 gatcgaactt atcacgcagt gccagcaggc ccgccatcgc acactcggag gagcagtcca
     3781 tacggatcgc gccgtcttta tcaaggtgca taaagcggaa ggtttgatcg acctgatcgt
     3841 taacgatggt caggttgagg ttgtaatact cgccaatacg cttccagtat tcgataccgg
     3901 aaccgcccag cggatcaacg cccagcgtca ggcccgcttt ctgaattgcc gccatatcaa
     3961 cgatatcggc cagaccttcc acaaacggct gcaccagatc ctgctctttt acatgaccgg
     4021 atgccatcgc ttcgtcgagg gagatacgct tcacgccttt caggccatcg gccatcagtg
     4081 cgttggccct gtcttccacc actttggtga cgttggtatc agccgggcca ccatttggcg
     4141 ggttgtactt gataccacca tcttccggcg ggttatggga cggtgtaatc acgataccgt
     4201 ctgccagcgg gccacctttt ttattgtgaa ccaggatggc attggaaatg gcaggtgtcg
     4261 gagtgaagcc attgttttcc tgcacaatga catcaacgcc gttcgctgcc agcacttcca
     4321 gtacggaaat gaacgcaggc tcggacaggg cgtgagtatc tttacccaca tagcaagggc
     4381 cagtgatgcc gtttttcgca cgttcttcag caattgcctg agcgatcgcc agaatgtgcg
     4441 gctcgttaaa gctgtggcgc gctgcactgc cacggtgacc ggaagtaccg aatttcaccg
     4501 cgtgctccgc attccctgct tctggtttca gtacataata ttgcgccgtc agttgggcga
     4561 cgttaatcaa atcactctgt tgtgcaggtt ggcctgcacg attgtggatt gccattgctt
     4621 tgtcctttgt ctgcaacgtt ttagatagtt ccgcaaacct tctcaatcaa ttccgccggg
     4681 aattgcatcg actgcatgat gtgttcgatc atgctgcatt tacggccggt gttggtgttg
     4741 gtgatcaccc aatacggcgt gcctggcaca tgtttcggct tggtctgatt accatttttc
     4801 agcagcgttt gttcatctgc cgcaaagtaa acgcgtgtac gaccgtgcaa cgattccgtt
     4861 gcttcggcaa acgcctgggc gtcaagagaa tatagtgtag acaacagcag cataaagcga
     4921 ttgaccgctc gcttttgctc tgcgtattca tccgaaagca gaagttcacg cattgcgcga
     4981 accttgtctt taatcgtttt gaccggcttc gcttcgacga tagcaggtga cgcaacgcga
     5041 acctctttcg tcaccggagc agcaggctgt gatgcggcgg aaaatttcaa catacgccgt
     5101 aaaatgtcgg atgcgctctc gccgatatgc ttagtgtggc tggcaatata gctgtagagt
     5161 tcatcatcaa cttcaatcgt tttcatctta atccagtgca gtgtcttagc tgaatacaag
     5221 tcgccaggag tatatgaata aatcccggca gcggatagcg tcaagcctgg cttgcagaaa
     5281 aactcggaat aaaccttatt tgttgcagcc ctgtggcaca atggtcaaca taatacccta
     5341 acccgacaac gcgaaaaaaa gaactttgcc atgaaattga atatccgcgc gcaaactgca
     5401 caaaaccagc acaataattc tcccatcgtt cttgtccatg gtctgtttgg tagcctcgac
     5461 aaccttggcg tactggctcg cgatctggta aacgatcaca atatcatcca ggttgatatg
     5521 cgtaaccacg gtctttcacc gagagatccg gtaatgaatt acccggcgat ggcgcaggat
     5581 cttgttgata ctttggatgc acagcagatc gacaaagcaa catttatcgg tcactccatg
     5641 ggcggtaaag cggtaatggc acttactgca ctggcccccg atcgcatcga taaactggtg
     5701 gcgatcgata tcgcaccggt cgactatcac gtacgccgtc atgatgagat tttcgcggct
     5761 atcaacgcgg tcagtgaatc ggacgcacaa actcgccagc aagcagcagc aataatgcgc
     5821 cagcatctta atgaagaagg ggtgattcag tttctgttga aatcttttgt tgacggggag
     5881 tggcgcttta acgtgccggt attgtgggat cagtatccgc atattgtggg ttgggagaaa
     5941 atcccggcat gggatcaccc tgccctgttt atccctggcg gcaattctcc gtatgttagc
     6001 gagcagtacc gtgatgattt actggcacaa tttccacagg cacgggcgca tgtgattgca
     6061 ggcgcgggtc actgggtcca tgctgaaaaa ccggatgcgg tattacgcgc tatccgtcgc
     6121 tatctcaatg attaacgcaa ctgattaaaa acccagcgtc cgcgtcgctt tccgcgcgtg
     6181 ggcgttggcg tgactcatcg cgctgatgta ttatggcgcg ctatccatcc gggcagaggc
     6241 ctggctgatt gtttcccccg aagtcaccaa gatcatggcc aaagaacaaa cggaccgtac
     6301 gacattagat ctgttcgcgc acgagcgtcg accgggacga ccgaaaacta atccgctttc
     6361 gcgcgatgaa cagctgcgta ttaataaacg caaccagcta aaacgcgaca aagtacgtgg
     6421 ccttaagcgt gtcgaactga agctgaacgc ggaagctgtc gaggcgctga acgagctggc
     6481 ggagtcgcgc aatatgagcc gtagcgaact gatcgaagag atgttaatgc agcaactggc
     6541 ggcgctgcgt agtcagggca tcgtttaaat ttccactttc atgtagcaca gtgtgcagtc
     6601 ctgctcgttt gctgattccg caagactgcc tgttctgcta tgattgcctt tatccgtggg
     6661 caattttcca cccccatttc aataagtttc aagaggttat ttcactcatg gctatcactg
     6721 gcatcttttt cggcagcgac accggtaata ccgaaaatat cgcaaaaatg attcaaaaac
     6781 agcttggtaa agacgttgcc gatgtccatg acattgcaaa aagcagcaaa gaagatctgg
     6841 aagcttatga cattctgctg ctgggcatcc caacctggta ttacggcgaa gcgcagtgtg
     6901 actgggatga cttcttcccg actctcgaag agattgattt caacggcaaa ctggttgcgc
     6961 tgtttggttg tggtgaccag gaagattacg ccgaatattt ctgcgacgca ttgggcacca
     7021 tccgcgacat cattgaaccg cgcggtgcaa ccatcgttgg tcactggcca actgcgggct
     7081 atcatttcga agcatcaaaa ggtctggcag atgacgacca ctttgtcggt ctggctatcg
     7141 acgaagaccg tcagccggaa ctgaccgctg aacgtgtaga aaaatgggtt aaacagattt
     7201 ctgaagagtt gcatctcgac gaaattctta atgcctgatg tgatgcggcg tagactcatg
     7261 tctacgccgt attaatagat aatgccaatc aaaataattg ctacaaattt gtaacttttg
     7321 ctgttgtacc tgtacaatgt cccggtgttc aagtggcctt gccgttgtaa atgtaagctg
     7381 tgccacgttt ttattaacaa tatttgccag ggacttgtgg ttttcattta ggcgtggcaa
     7441 ttctataatg atacgcatta tctcaagagc aaattctgtc acttcttcta atgaagtgaa
     7501 ccgcttagta acaggacaga ttccgcatga ctgataacaa taccgcccta aagaaagctg
     7561 gcctgaaagt aacgcttcca cgtttaaaaa tcctggaagt tcttcaggag ccggacaacc
     7621 atcacgtcag tgcggaagat ttatacaaac gtctgatcga tatgggtgaa gaaattggtc
     7681 tggctacggt atatcgcgta ctgaaccagt ttgacgacgc tggtatcgtc acccgccaca
     7741 attttgaagg cggtaaatcc gtatttgaac tgacacagca acatcaccac gatcacctga
     7801 tttgcctcga ctgcggcaag gttatcgaat ttagtgatga ttccatcgaa gcgcgtcagc
     7861 gtgaaattgc cgcaaaacat ggcattcgcc tgactaacca cagtctctat ctttacggtc
     7921 actgtgccga aggcgattgc cgcgaagatg agcacgcgca cgaaggcaaa taagccagcc
     7981 tgaacgagaa aagccaacct gcgggttggc ttttttatac aagggaaata agaaaagcga
     8041 gagttacagc tctcacttat ttgttattac tgcgtatttc ctgccagacc ttatcacaat
     8101 cggctttcac cgcctgatca tttccggttt gcgttgcacg cagacactgc tgataatcca
     8161 gtacgcgtac actttcctgg tcggcaaact gctgatgttg tttctctttc ttcagcacgt
     8221 ttaacacgct ctggcaggct tcaatttttt ctggcgaacc ttgtgcggtg ttgatacagg
     8281 cgctgtatgc ctctttcaga cgactgtctt cctgtggcgc ggttgattgc gcacaagcta
     8341 ccagccccga tgccattatg gcgatgagaa tcagtttttt catagcaaat tcctcaaacg
     8401 tgcggcaggc acaaaacctg ccgcgtcggg catcagaaga tggtgaatgg tgcgattacc
     8461 ataaatttca cgtcacgctc atcctggaag atgttaccgt aaccgccgcc ccagcttggg
     8521 atgtcagagt ggttgtcgta ttcggtgaag tgcagtttga acatcgtgcc tttggcgcga
     8581 ccgtcctgaa tggtgtagac cgcatccagg ctgtaggcag actcttcaat agtccggttt
     8641 ttgtcgtagt acgcattcgg attgctctgc caggtcgcag gtttagcatc ccatgcgtaa
     8701 acgtaggaag cgccgatggc gaagcctgga agattccagt ttttcaggtc atacatcgca
     8761 ccgaagaaga ccgctttttc gccgttggcg ttgaagtcgg aacggttatc ccaccagata
     8821 tccaggcggc cgttggagga agcgtaggtt ggagtcatac gttgcaggaa gtatccctgc
     8881 tgaccgtcag ccttaaccca ggtgccttcg aggcgcaaat ccactacgtc agccgcccgg
     8941 taaccaaagg tcaacgcttg cagccaggcg gtgctgtcat aaaggtcgtt gacgctgcga
     9001 tcgtcaactt tatcgcgcgt accatagaac tggtagctgg tggttaacgg gctaccggcg
     9061 atatcaaatt tgtagctggc tttggcaaaa tattgatcga tatacccttc cgcctgacca
     9121 aatgccgctt ccagtacgaa gttatttttg aagtcgtatt tcgccccaag ggagtgcaga
     9181 taatcaactt tggtggtttt atcgttctga taaaactcat ccatttccag atgccacggc
     9241 gctttgtatt cgttggtcca catgtaggag aaactcaacg caccagcatc gccgtaatca
     9301 aaattcgccc cggcttccgc cccctgataa gtacctggca taaagctcca gtgcggcgct
     9361 aacagcgttt gaccagttgg ctgaatgtaa cctgccctcg cccaaaccgg accgtattta
     9421 aatttggccg cagctttata caggcttata ccgcttttgt cgccggacca gtcttcgtca
     9481 taggctttat tactttttga aaacgcgatt tcgttcgggt gggagctgtc gccgttttcc
     9541 gccatttcaa tcgccgtaaa cgcagcaata tcaaggccga acatatcagc agcataaccg
     9601 gactgaaaat cgaggttggc attccaggtg gagtgagaaa ggttggtttt gtatttgtcg
     9661 ccgtcggtaa catctttacg gtcgcgttca cgctgccagt aatatatacc gccggttaag
     9721 gttgaatcgt cgatgaatcc ttcggcgcga gcctccggca ttgccataaa gcccgacatt
     9781 gctgtaacac cggcgatagc cagcgccagc gtactacgtt tgccactaaa cgtacgcatg
     9841 ggttaatcct ctttgacgta taaattgctg caccaaaggt gaacagcgaa aaaataaaaa
     9901 ttaaaaaagt agcgttgaga aaagtctcgc tgactacagg aacagactat ttttcgcgac
     9961 gcgaattatc aatctctttc tgtaaccaga atgtaagaac atgagcaacc gcacatattt
    10021 tgatgatttt tgttacaagg cttaagtttg cggttgaaag gcttgcagaa gaataaaagt
    10081 gtgaattt
//
LOCUS       DAZGAN020000154         9880 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGAN020000154 DAZGAN020000000
VERSION     DAZGAN020000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.154"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(270..345)
                     /locus_tag="ACHKYO_RS22290"
                     /old_locus_tag="pgaptmp_004458"
     tRNA            complement(270..345)
                     /locus_tag="ACHKYO_RS22290"
                     /old_locus_tag="pgaptmp_004458"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(310..312),aa:Asn,seq:gtt)
     gene            complement(446..1933)
                     /gene="yeeO"
                     /locus_tag="ACHKYO_RS22295"
                     /old_locus_tag="pgaptmp_004459"
     CDS             complement(446..1933)
                     /gene="yeeO"
                     /locus_tag="ACHKYO_RS22295"
                     /old_locus_tag="pgaptmp_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HGU4431188.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
     gene            2094..2169
                     /locus_tag="ACHKYO_RS22300"
                     /old_locus_tag="pgaptmp_004460"
     tRNA            2094..2169
                     /locus_tag="ACHKYO_RS22300"
                     /old_locus_tag="pgaptmp_004460"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2127..2129,aa:Asn,seq:gtt)
     gene            complement(2207..3157)
                     /gene="cbl"
                     /locus_tag="ACHKYO_RS22305"
                     /old_locus_tag="pgaptmp_004461"
     CDS             complement(2207..3157)
                     /gene="cbl"
                     /locus_tag="ACHKYO_RS22305"
                     /old_locus_tag="pgaptmp_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HGU4431189.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRRGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3259..4176)
                     /gene="nac"
                     /locus_tag="ACHKYO_RS22310"
                     /old_locus_tag="pgaptmp_004462"
     CDS             complement(3259..4176)
                     /gene="nac"
                     /locus_tag="ACHKYO_RS22310"
                     /old_locus_tag="pgaptmp_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HGU4431190.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRKKRGVTPTDTGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4503..4578
                     /locus_tag="ACHKYO_RS22315"
                     /old_locus_tag="pgaptmp_004463"
     tRNA            4503..4578
                     /locus_tag="ACHKYO_RS22315"
                     /old_locus_tag="pgaptmp_004463"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4536..4538,aa:Asn,seq:gtt)
     gene            complement(4634..5569)
                     /gene="ldtA"
                     /locus_tag="ACHKYO_RS22320"
                     /old_locus_tag="pgaptmp_004464"
     CDS             complement(4634..5569)
                     /gene="ldtA"
                     /locus_tag="ACHKYO_RS22320"
                     /old_locus_tag="pgaptmp_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4431191.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5631..6710)
                     /gene="cobT"
                     /locus_tag="ACHKYO_RS22325"
                     /old_locus_tag="pgaptmp_004465"
     CDS             complement(5631..6710)
                     /gene="cobT"
                     /locus_tag="ACHKYO_RS22325"
                     /old_locus_tag="pgaptmp_004465"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HGU4431192.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMLGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6722..7465)
                     /gene="cobS"
                     /locus_tag="ACHKYO_RS22330"
                     /old_locus_tag="pgaptmp_004466"
     CDS             complement(6722..7465)
                     /gene="cobS"
                     /locus_tag="ACHKYO_RS22330"
                     /old_locus_tag="pgaptmp_004466"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HGU4431193.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLVKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7462..8007)
                     /gene="cobU"
                     /locus_tag="ACHKYO_RS22335"
                     /old_locus_tag="pgaptmp_004467"
     CDS             complement(7462..8007)
                     /gene="cobU"
                     /locus_tag="ACHKYO_RS22335"
                     /old_locus_tag="pgaptmp_004467"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HGU4431194.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHETVLLECVTTMVTNLLFDYGGDKDPGE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(8141..8500)
                     /locus_tag="ACHKYO_RS22340"
                     /old_locus_tag="pgaptmp_004468"
     CDS             complement(8141..8500)
                     /locus_tag="ACHKYO_RS22340"
                     /old_locus_tag="pgaptmp_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HGU4431195.1"
                     /translation="MHCRGCGFIGEEWQQDFQPLDGGPVQSLSDAYSDYSVLLLIAAE
                     SEISVPEWFPHSEGTTVILIAEHNARLLTGPAGGAVALIHYVNEICHSRETAVNDAFC
                     RTLTRGSLAIPQCHLLS"
     gene            8479..8775
                     /locus_tag="ACHKYO_RS22345"
                     /old_locus_tag="pgaptmp_004469"
                     /pseudo
     CDS             8479..8775
                     /locus_tag="ACHKYO_RS22345"
                     /old_locus_tag="pgaptmp_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
     gene            complement(8876..9205)
                     /gene="tmaR"
                     /locus_tag="ACHKYO_RS22350"
                     /old_locus_tag="pgaptmp_004470"
     CDS             complement(8876..9205)
                     /gene="tmaR"
                     /locus_tag="ACHKYO_RS22350"
                     /old_locus_tag="pgaptmp_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HGU4431196.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(9377..>9880)
                     /locus_tag="ACHKYO_RS22355"
                     /old_locus_tag="pgaptmp_004471"
     CDS             complement(9377..>9880)
                     /locus_tag="ACHKYO_RS22355"
                     /old_locus_tag="pgaptmp_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431197.1"
                     /translation="QSAFSPNLLERPRLESHLQKLLTDAVKMRGLIAPASKESRIPKS
                     IYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQQILLSLVHALY
                     EGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNMETAHQLELLSNL
                     ICRALRK"
BASE COUNT         2686 a         2616 c         2169 g         2409 t
ORIGIN      
        1 tagtttcccg ataaagacgc gatagcgaca tcccgcataa ggcatttttc tctttatctt
       61 tgtacggtac ttcatggaac agagtttttg accttgcgaa tcgtgatgtc tgttgggggc
      121 aacttgctca ctgaagcgtg agactcgatt aagcgcacga aacacagaaa tcaaaaaacc
      181 cggtcacttt tttacaaggt aaccgggtaa aaataatttt tattttttaa ctgttttgag
      241 actcatagag atgtctcaaa actaaaattt ggctcctctg actggactcg aaccagtgac
      301 atacggatta acagtccgcc gttctaccga ctgaactaca gaggaatcgt gagaacgagg
      361 cgaatattag cgatgcccac ccataatgtc aaagcctgtt ttttaaattt gaaatcgttt
      421 gctgaaataa tctgcatttt gtcgtttatt ccgacacagc tggctttttt tcacactttt
      481 gcggttcgga tcgagggtat ttccacagcc aacgtccagt aaccattcgc cagtaaaaca
      541 gcacggcccg cacagcccag tcggcaaaca ttcccatcca gacaccaacc acaccccagc
      601 caagcatgat gcccagcaca taaccgacga caacccggca accccacatg ctcaacatag
      661 aaacccacat ggcgtaacgg gcatcgcgag cacctttaaa tccagcgggt agcacccatg
      721 aggcggacca aataggcata aataaagcat ttagccaaat cagaatcaca acgacatgtt
      781 taacctgtgg atcctgggtg taaaacgatg ccataacccc ggcaaaggga gccgttagcc
      841 aggcgatggc cgttaatcca agagtggaaa gccagaacac atgccgcaac tgaatctctg
      901 cttgcgctat ctgccctacc cccaaccttc ggcctgtaat gatcgtagaa gcagagccga
      961 gcgcacttcc gggtaagttg ataagagccg caattgaaaa cgcgataaaa tttccggcaa
     1021 taacactggt ccccatcccg gcaacgaaca tttgggttaa taaccgacca ctggtaaata
     1081 acactgattc gacactcgct ggaataccaa tccccatgac ttcccagata atgctaaaat
     1141 tcagcggttt aaaatagctc tttaacgaaa tccttagcgc aggattaaaa ccaatcgcca
     1201 gcacccacaa aattgcaact gcgccaatat aacgagaaat ggttaaaccc aacccagccc
     1261 cgacaaatcc cagtcccggc caggagaaaa ggccgtaaat caatatgccg ctaataataa
     1321 tattaagaat attcaggcta ccgttaatca gtagcggtat tttcgtattc cctgcaccac
     1381 gaagtgcccc gctaccaatc aaagtgatgg cagctgctgg ataactgagt accgtcagct
     1441 ccagataagt caacgccagt gctttaactt ctgtcgtggc atcacccgcg acgaaatcaa
     1501 taatttgttc gccaaaatga tgaataagcg ttgccaacag tacggcaaac aacgtcatga
     1561 tcaccaatga ctgccgcgtc gccaccctcg ctcgtcgtcg atcccgctta ccgagactaa
     1621 atgccacaac gacagtagta ccaagatcga tagctgcaaa aaaagccata atgaccatat
     1681 tgaagctgtc cgccaatccc acgccggcca tcgcatcttt tcccagccag ctgaccagaa
     1741 aagtgctcag gacacccatc aacaggacac aggcattctc catgaagata ggaacagcaa
     1801 gcggggttat ctcgcgccag aacaacactt tgtagctctt gcgtttagcg tgccagcgag
     1861 tgccgtgaac aacctggcgt aaagcagagg agatattcaa agccgacctt aattgcagaa
     1921 agtgaaacca catttcaaat aatgagggag aatcagcaaa gctgcaaaga ttttcgccaa
     1981 caaattgtct gcaaatgcaa caaactgttg atagaaacgg caaacagttg gggaatttaa
     2041 aaatcgggtt tgacaaaaga tttttcgccg ttaagatgtg cctcaacagc gattcctctg
     2101 tagttcagtc ggtagaacgg cggactgtta atccgtatgt cactggttcg agtccagtca
     2161 gaggagccaa atttctgaaa agcccgcttt tatagcgggc tttttgctat atctgataat
     2221 caatttcctc ttcactgttt tccatcacct gccgcttgat atcctcaact gacagtcctg
     2281 cattacaaag ttccagaaag cgccagacat agttacgctg aagttgtcct cgcttcagtc
     2341 ccaaccagac agtattagca tcgaaaagat gccgcgtatc cagacggatt aaattctctt
     2401 cctcttgttc gccgctggat tgttcggcaa ctaatccgat cccaagccca agagcaacat
     2461 aggttttaat gacatcagaa tcctgcgcac ttaatacaat atctgccagc aaacctttgc
     2521 gggcaaatgc gtcatcaata cgtgagcgcc ccgtaatccc ccgtcggtaa gtgattaacg
     2581 gccacttcgc tattgattcc agcgtcaatg gtgtaatttg cgttaaggga tgatcgagtg
     2641 gaacaagcaa actatggtgc caacgaaacc acgggaaggc gacgagctgc gggtcattac
     2701 tcaaacgctc gctggcgata ccaatatcag cttcgccatt ttgcaacaac gtcgcaattt
     2761 cctgtggcgt cccctggatt agctcgagcc gaacctccgg gaaaagttcg cgaaaagctt
     2821 taatgacctc tggcaagcta taacgtgcct gagtatgcgt cgttgcaata gtgagaacgc
     2881 cagacgtatc gttggtaaac aggtctgcaa gccgacgaac attactggct tcattcagaa
     2941 tacgttctgc aatgaccagt aatgctttgc ccggttcagt catgcccagc agtcgcttac
     3001 ctcgtcgaac aaatatttcg atgccaagtt catcctccag ttcccgaata tgacggctga
     3061 cgcctgactg tgaggtaaaa agcatattcg caacctctgt caggttgtaa tcctgacgtg
     3121 cagcctcgcg gattatcttt agttgttgga aattcacggt aaactccggg cagttcagat
     3181 ttcccgttat tgttaaagtc taatgcccgg cataacaaat aataaaaacc cgcatcttat
     3241 tccattccga tataacactt agctcaccaa ttgccactgc cttttttcca tcactggaga
     3301 actaatcact gacattaaca actctttcac tgcctgtgcc tgtggcgata agttcgctct
     3361 ggcgggtaaa tttaatgaca aagagagact catggaagga gtggtaatgc gtgacatcca
     3421 cccatttact gcgccacata acgaacgcgc ggccgattcg ggtaatactg caacgcccat
     3481 gccgctggca atcgctgcgg taagcgtggc aatagactca atttcaccaa taacttttgc
     3541 cgtgagtcgc cgtagggaaa aagcctcatc aacacgaagt ctaatagcac tgtaatcact
     3601 ggggagaaag aggttcattt gcgcaatagc attcacatca acgctttgcc ccggacaatc
     3661 ttgagttcct accaggaaaa gatcttcttt cagcaaagcc tgactggata ccccagccac
     3721 aggggaatgc tcataaatca ccgccatatc gagttggtga tttatcagtt tttcgttaag
     3781 cactgcaccg ctattttcat gaagatagat aacgatctcc gggaattcag cgcgaaccgc
     3841 ctgtaataag ggcatggtga tggatgacgc agcggttcct ggcgcaaagc caatcgagac
     3901 ttgccccgat aatgcctgac caacgttatg caccgccagt tgggcctgct cacactgacg
     3961 taaaatggcc cgcgcatggg tatagagaat ttttccggtg tctgttggcg taacgccccg
     4021 ttttttgcga atcaaaagtt gttgatttaa ctcaccttcc agtgtggcaa cctgctggct
     4081 gagcgctggt tgtgcaatat gcaatacttc agcagcctgg gtcaggctac caatatctac
     4141 aatttttacg aagtatttca ggcgtctgaa gttcatgttg cctccggttt ttaagaatcg
     4201 gcccaagtgc cgccattact tacaaccaga ctgcaagatg cttgccagtt ttattttggc
     4261 gttgatgtac aagctaacca actgtcaaat aagagattat gatagattcg tcatttgctc
     4321 ctttaatcag ctgtcgcgtt cccctgccct ataaaaggag ggtatgcacc acgatgattc
     4381 attacccaat aagattgaaa gctcaccact ttgttgaaat tgacagcaaa caaacaaaaa
     4441 aatgcatttc accctttgac atcaccatgc actgccatta atatgcgccc cgttcacacg
     4501 attcctctgt agttcagtcg gtagaacggc ggactgttaa tccgtatgtc actggttcga
     4561 gtccagtcag aggagccaaa ttcaaaaaag cctgctttct agcaggcttt ttgctttcta
     4621 attaccaacg ctcttaaaac atctgtcttg aaccagaact aatttgcaca ggcattcccg
     4681 atcgacgttg caacgcagca tttgcgcgat ttacatcaac ttcttgcccg ctgataaacg
     4741 cccgcaaaga tggggttacc ggcaatggca cttttcggtc agactcatat tctgcacgat
     4801 tgcgcgacaa cggctcatga acttccagcc agttcgagcc atctggttca gtggtgtatt
     4861 ttactggctg gtcgataatt tgcacacgcg tcccaacagg aacattatca aacagatatt
     4921 tgatatcgtc attgcgcaga cgaatacagc cctgacttac ccggagccca ataccaaaat
     4981 tggcattggt accatggatg gcatacaacc tgccaatata aatcgcgtac agccccatgg
     5041 gattatcggg gcccgcagga acaaatgcgg gcaaactctc ccctcgtttc gcatattcgc
     5101 gccgagtgtt cggcgttggc gtccaggttg gagcttcttg tttacgttca acggtagtca
     5161 cccagttacg cggggtttct cgcccagcct ggccgatacc aataggaaag acttccacag
     5221 tattactgtc tggtgggtag taataaagac gcatctcagc gacgttaacc acaatccctt
     5281 tacgaacagt ggcgggcaaa atcagttgct gcggaatggt gagttgcgag ccagacttcg
     5341 gcaaaaaaac atcagcgccc gggttcgctt ccagcatgtt acttaaccct tgcccgtatt
     5401 gtgcggcaaa agtctccagc ggctgggtat tgtgatcagg aacagttaca gtaaacgact
     5461 gccccactaa acggctaccc tctggaggta atggataagt taccgccagg ctagtatggc
     5521 tggcaaaaag tagagcaaat gagcaaaaaa tatttacacg acgcatcatg tccctttcct
     5581 aagtcgcgaa agctatccgt taattatagc ttttatcaga cttttcgttt ttaactgttc
     5641 aaatcagaag tcgtattccc cggtagaaca atattactgg cagcaagttc gcccatgttg
     5701 ttatatatcg cacaggcagc ttcgatgatg ggcatcgcca gagctgcgcc actcccctca
     5761 cctaaacgca tctccatatt gagataaggc tccagcccca aatgcgacag cgcaatacgc
     5821 gccccttttt ctgccgacag gtgagaagga atgagatacg gtttgattgc aggagacatc
     5881 tggcaggctg cgagcgcggc agcataagaa agaaatccat ccagcaacac gggtaaaccg
     5941 caggaagcag cgcctaacat cacgccagcc attccgacca aatcaaatcc acccactttt
     6001 gccaggacat caacgccatc ctgcggattt ggttgattca acgtaatcgc ccggcgcaca
     6061 acatcaattt tattagccag tttatctgtc ggcaggtttg cgccaatccc aaccacttct
     6121 tcaggagccc ggccagtgat tgtgctgact attgccgctg ccggtgtcgt gtttgccatc
     6181 cccagttcac ctacaccaaa cagcgtaaca ccgttttttg ccagctcccg cgtataacat
     6241 atgacgtcca aaagcaactt ttctgcctga tggcgactca ttgccggagc tgaagcaata
     6301 ttgccactac ctcgagcaac acgcatgttg ataagcccgg ggataggctc agcagtatca
     6361 ataccaacat caattacgta gacgttagcg cccgcttgtg ctgccagcac acacacgccg
     6421 gttgttccgc gggtcatatt ttcagcctga atggctgtca cttcttttgg ggaaatagcg
     6481 accccttcct cccagacgcc gtgatcggca cacatgacca gtaccgcttt tttgcccaca
     6541 tgcggaacgc cattcaaccc cagcattcct gccagttgta tggcaagcgc ctccagcctt
     6601 cccaggctac caacaggttt gagtaacccg tcgatatgcc gttgtgcacg cgacatagcg
     6661 gcaggatcga tagcagggat cgtattcagt aaatcggcaa gtgtttgcat gtcacgtctc
     6721 tttatagcag tgccagcagg aagaccagtt caccaagctc tatagcagct cccagcgtat
     6781 cgccggtttg cccgcccagc gtacgtttga gaagctgacc gaggatgaaa atcgccacca
     6841 tcgtcaccac catagcagcc acaccatgca tacccggcaa taatacggca gcaaaaatag
     6901 cggctaagcc gagtgtgacg caggtttgtc gcccatcaat tttgccaatg aacacattgc
     6961 caagcccttc ttcccgggca taacgatgac gatacatcaa taatgcggca gtcccgcggc
     7021 tgaccgcaca tgctgccgcc agcgatgcaa ggatcggttc gccacgcaga gccaactcgc
     7081 ttaacaccag aatctttacc agtacaacaa aaatcaatgc cagaccgccg tgggtgccta
     7141 aacggctatc acgcatgatt tccaacatgc gatcgcggct acgtgcagaa aatacgccat
     7201 cacaggtatc ggcgaggccg tccaggtgga atcccccggt catcagcgcc agcacaagta
     7261 cgctaaacag tgccgccagt ggtacgccac accatgcctg cagcaccatg aagaccagcc
     7321 cgctaatcgc gccaagcaat aatccaatta aaggaaaagt aataataccg cgagaataat
     7381 gctcgaaatc cagtccctgg gaccagcgac gcggtacagg cagacgcgta ataaaagaga
     7441 gcatcgccca aaataattta ctcatttgat ttttactcct atacccgaaa ccaccagcca
     7501 tacttcattt gccgcagcgg ccagctgctg atttacccgc ccggcaatat cacgaaaatg
     7561 tcgtgccaga cgactctccg gcacaatccc cattcccact tcgttagtca ctaatacaac
     7621 ctttgcggga caacgttggc aggcagcaat caacgactga atctcagcat taatcgcctg
     7681 ttccatcgcc tgataatccc attcaccagg gtctttatcg ccgccataat caaacaacag
     7741 attagtcacc attgtggtaa cgcattccag caatacagtt tcatgcgggt taatgtctgc
     7801 atgaattaat tcatcaagat gttgccaacg ctccaccgtg cgccagtgcg ccgggcggcc
     7861 ttgccgatga tgttctatcc gtgcagccat ctcatcatca aggatttgtg aggtggcgat
     7921 atacagaacc tgtgaagagt ccccaataag cgcctctgcg tggcgactct tcccgctccg
     7981 tgcgcccccc gtcaccaaaa tcatcatacc gactcctgat tcaattgtat gatggtattc
     8041 attgaacaga acaatggatc ccccaccgtt gctgaccgca aaacatttac gcgattaata
     8101 ctgcgcgtaa tataattaaa aatccagcat tctcaatcca ttacgaaaga agatggcatt
     8161 gcggaatagc cagacttcct cgtgttaacg tccgacaaaa tgcgtcattg acagcggttt
     8221 ctctggaatg acagatttca ttcacataat ggattaatgc tacagcccca ccagccggac
     8281 cagtcagcaa acgggcattg tgttctgcaa tcaaaattac cgtcgttccc tcagaatgag
     8341 ggaaccattc tggtacggat atctcgcttt cagctgctat caatagcagc actgaataat
     8401 cgctatacgc atctgataac gattgcacag gcccaccatc aagaggctgg aaatcttgct
     8461 gccactcctc accgataaaa ccgcagcctc tacagtgcat tgttggtcag tgcctgcgaa
     8521 gcagacatta tcgcgctggt gcttaatgcg gacgcaccgt ggtcaccttt ttcgccaggc
     8581 tttacctcgt caatgaatcg cccggtcatt ggagtagtca ccaaagcaga tttagccagc
     8641 atggaacaaa tatctttggt gaaatgttgg ttacgtgaag ctggagcgca caacgtgtta
     8701 gtaaccagtg cggttaataa caatggagtt gcagaacttt ttgccttgct gcatacagaa
     8761 gaaggctgtc gctaatttcg acaggttctc agatgagaat gatgacggtg atacacagaa
     8821 aagaatcaat gagcaatgag ggttgccagg caaccctcat tgaataaaac aggaattact
     8881 tcgcttcgcc gtttttcatt tcgcccatag ctttcagctt tttggagata tcgcggcgtt
     8941 ctttggagag ctcggcattt ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt
     9001 tcatgctggc gatgatgccc tggattgctt caacgctcat ccccggcttg atgtaatcgc
     9061 tcaggttgtc cagcagcagg acgcgcttct ggttgtcacg gatctttttc tcaacgtcct
     9121 gaatttcacg ttgcagtttg ttcttacgac ggaacagacg aacaaattcc agtacgtcct
     9181 ggaatgaagg cttggtagtt tccattttta tacccctgat aatgtgagag tcggattcgt
     9241 ttaagcaacc gctattcgtt agggccaaca ttactcatag ctgccgcaga tgacaatgct
     9301 tttatccctt ttcactatca taccctttat ccttgctgaa tcgaagcagc agcaagatga
     9361 ttctgaagtt caggaattat ttgcgcaagg cccggcaaat cagattcgat agcaactcaa
     9421 gctgatgtgc cgtttccatg ttcagccaca cataaccata gattggtgtt tccacaacct
     9481 tcaggtcatg gtggttattg agcaactgac gcagctcttc cacagcatcg ttcaattttt
     9541 ccgtattggc aaaaaccggc tgcggattac cttcgtacag cgcatgaaca aggctcagca
     9601 gtatttgctg catcatgtgc tgggtatcac gaagtttttg cgcgttcaat aacacgaaat
     9661 ggctggggcg cgtggcccag tacgcattga tttgcaactc cagcatacaa accagattgc
     9721 gattaatggt ctgaatacct tcatatatcg attttggaat acgactttct ttgctggcgg
     9781 gcgcaatcag tccacgcatt ttcacggcat cggtcaggag tttttgtaga tggctttcca
     9841 gacgtgggcg ttcgagtaag ttcggtgaga atgcagattg
//
LOCUS       DAZGAN020000155         9829 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGAN020000155 DAZGAN020000000
VERSION     DAZGAN020000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.155"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(155..328)
                     /locus_tag="ACHKYO_RS22360"
                     /old_locus_tag="pgaptmp_004472"
     CDS             complement(155..328)
                     /locus_tag="ACHKYO_RS22360"
                     /old_locus_tag="pgaptmp_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431198.1"
                     /translation="MQVDIISPVTDPYACAHQALLALINAGKIENSAMCFEELKQLVR
                     LYEYIKKQSQDTA"
     gene            complement(453..581)
                     /locus_tag="ACHKYO_RS22365"
                     /old_locus_tag="pgaptmp_004473"
     CDS             complement(453..581)
                     /locus_tag="ACHKYO_RS22365"
                     /old_locus_tag="pgaptmp_004473"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431199.1"
                     /translation="MEEAPAILTAAPFRGLPQPEAHDRIKFNDKPYILAVRFRVMP"
     gene            868..1056
                     /gene="dicB"
                     /locus_tag="ACHKYO_RS22370"
                     /old_locus_tag="pgaptmp_004474"
     CDS             868..1056
                     /gene="dicB"
                     /locus_tag="ACHKYO_RS22370"
                     /old_locus_tag="pgaptmp_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4431200.1"
                     /translation="METSLPNVNTSEGCFNIGILLSNREFTEDAIRMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            1053..1241
                     /locus_tag="ACHKYO_RS22375"
                     /old_locus_tag="pgaptmp_004475"
     CDS             1053..1241
                     /locus_tag="ACHKYO_RS22375"
                     /old_locus_tag="pgaptmp_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4431201.1"
                     /translation="MTSAFALMMTVFLITGEPQNVITGIYASKESCHQARDEQKISGE
                     CLPLNKVSLYLNNETPAG"
     gene            1337..3808
                     /locus_tag="ACHKYO_RS22380"
                     /old_locus_tag="pgaptmp_004476"
     CDS             1337..3808
                     /locus_tag="ACHKYO_RS22380"
                     /old_locus_tag="pgaptmp_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4431202.1"
                     /translation="MNKIFICAAIPDEQAIEEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NITFDNLAPEVQNAVMVKFDACENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPAAKWPEIQAEMRIWKKRRESERKETGKYISVVDIARS
                     RVNQQNTENAAEKTGAVTVAVRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALAYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSADAILSSAAQTDPVETAESNSQKNEILVEAEPSVEREGPFYF
                     VFTDKDGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLQQNTDTAEDSEQ
                     PEPVKVTADEVNKIMQAANISQPDTDKLLAASRGEFVEGISDPNDPKWVKGIETRDFE
                     NQNQPESEQNDQKAEQNSPNALQNEPETKQPEPEEQQEPEKVCTACGQIGGGNCPDCG
                     AVMGDATYQETFDEKNRAEVQEDDPEEMEGAEHPNNENAGNDQHHTSDSETGEAADPL
                     IAVNGHHVITSTSRVWIHLSVDLETMGTNPDAPINSIGGTFFDPATGEMGPEFSKAID
                     LETSGGIIDRKTIKWWAKRSREAQSAIFTDEISLDVALRLFIEFIEKNSGGCFVQVWG
                     NGANFDNVILRRSYERQGIPCPWLYYNDRDVRTIVELGNAIGFDVRMAIPFEGVPHNA
                     LDDARHQAKQVSAIWQKLIPSQADF"
     gene            3873..4121
                     /locus_tag="ACHKYO_RS22385"
                     /old_locus_tag="pgaptmp_004477"
     CDS             3873..4121
                     /locus_tag="ACHKYO_RS22385"
                     /old_locus_tag="pgaptmp_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4431203.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPGTLSKYAKAGMIFPLPKKVGRH
                     WRVDPRARFVGMVNKPEVIATDHPALKRILEDGAPTKI"
     gene            4099..5238
                     /locus_tag="ACHKYO_RS22390"
                     /old_locus_tag="pgaptmp_004478"
     CDS             4099..5238
                     /locus_tag="ACHKYO_RS22390"
                     /old_locus_tag="pgaptmp_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU4431204.1"
                     /translation="MARPRKYKTEVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKQAEAIAAEANSRLARQQMEQMEQMLTLQEKIIRDTGGSSTVSVFLNSYRKIQQERY
                     ENGEIKLNTLKQKASPLRVFDERFGTRPLDAITVKDVVSVLEDYKAKGHNRMGQIFRK
                     VLIDVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQR
                     GMLLALMTGQRLSDICKMKFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTQDDVV
                     SSCRDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFH
                     EQRSLSERLFREQGIDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(5284..5937)
                     /gene="ompW"
                     /locus_tag="ACHKYO_RS22395"
                     /old_locus_tag="pgaptmp_004479"
     CDS             complement(5284..5937)
                     /gene="ompW"
                     /locus_tag="ACHKYO_RS22395"
                     /old_locus_tag="pgaptmp_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HGU4431205.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSDGRKLAKGSYSDNVRLDPWVFMFSAGYRF"
     gene            6177..6266
                     /locus_tag="ACHKYO_RS22400"
                     /old_locus_tag="pgaptmp_004480"
                     /pseudo
     CDS             6177..6266
                     /locus_tag="ACHKYO_RS22400"
                     /old_locus_tag="pgaptmp_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            6294..7037
                     /gene="yciC"
                     /locus_tag="ACHKYO_RS22405"
                     /old_locus_tag="pgaptmp_004481"
     CDS             6294..7037
                     /gene="yciC"
                     /locus_tag="ACHKYO_RS22405"
                     /old_locus_tag="pgaptmp_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HGU4431206.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     ITQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            7067..7606
                     /gene="yciB"
                     /locus_tag="ACHKYO_RS22410"
                     /old_locus_tag="pgaptmp_004482"
     CDS             7067..7606
                     /gene="yciB"
                     /locus_tag="ACHKYO_RS22410"
                     /old_locus_tag="pgaptmp_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HGU4431207.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            7711..8108
                     /gene="yciA"
                     /locus_tag="ACHKYO_RS22415"
                     /old_locus_tag="pgaptmp_004483"
                     /pseudo
     CDS             7711..8108
                     /gene="yciA"
                     /locus_tag="ACHKYO_RS22415"
                     /old_locus_tag="pgaptmp_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
     gene            complement(8148..8867)
                     /gene="tonB"
                     /locus_tag="ACHKYO_RS22420"
                     /old_locus_tag="pgaptmp_004484"
     CDS             complement(8148..8867)
                     /gene="tonB"
                     /locus_tag="ACHKYO_RS22420"
                     /old_locus_tag="pgaptmp_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HGU4431208.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMAAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            8958..>9829
                     /locus_tag="ACHKYO_RS22425"
                     /old_locus_tag="pgaptmp_004485"
     CDS             8958..>9829
                     /locus_tag="ACHKYO_RS22425"
                     /old_locus_tag="pgaptmp_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4431209.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPAGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTKMVPGWIDPDTFKCNEHAETVRLIFKLLLDGESLHNIARHLQSNDI
                     KSFSRRKDANGFSVHSVRTILRSETTRGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPAS"
BASE COUNT         2709 a         2270 c         2389 g         2461 t
ORIGIN      
        1 aaagcgatga ttatttaaaa accataatta aaacaactca gctttctgaa gagctaccga
       61 agaaattgcc ataataatat gtttttctta tagaggggtt agaaatatgg gccagcatta
      121 tagcaggcac atataacaga aagtatttta aatattacgc cgtatcttgt gattgttttt
      181 taatatattc atacaagcgc acaagttgtt tcaactcttc aaagcacata gcggaatttt
      241 caatttttcc ggcgttgatt agtgccagaa gagcctgatg tgcgcaagca tatggatcag
      301 ttacagggct aataatatca acttgcatat tatcctccat agaggttccg ggttaaggat
      361 ggagaccaac acgctgtcac gtgtggtcgt gcgccggaca cggataagaa tccggcactg
      421 acagtttgct gaaagaacat atccctgaaa agtcagggca taacacgaaa gcgcacggcg
      481 agaatataag gcttgtcgtt aaatttaatt cgatcgtgcg cttccggttg tggcaatccg
      541 cgaaatggcg cggcggtaag tatggctggg gcttcctcca ttgctccaga aaatgcaccg
      601 ggttgtcagg ttgaccatgc gcctgagtga caacaccgcc acaacaacct ctgttatcac
      661 ttttctggtg attcggcgga aatggatatc cgccattttt aaagtgtatt ttgtgatgcg
      721 gtgaatgcgg ctaagcgcac gcggaacagt taaagctaaa aacagcgtta tgggtgattc
      781 tgtattccgg cgttaattgt taactggtta acgtcacctg gaggcaccag gcaccacatc
      841 acaaaattca ttgttgagga cgcgataatg gaaacgtcac taccaaacgt taatacgtct
      901 gaagggtgtt ttaatattgg tattctgctc agtaaccggg agtttactga agacgccatc
      961 aggatgagaa aatatgagcc ttatcttctc aatgataatt ccatactctc cagaattgcc
     1021 cttcttaaac ttggtatttt cggggggcag cagtgacttc tgcatttgca ctgatgatga
     1081 cggtttttct tataacaggt gagccacaga atgtgattac cggaatttat gccagtaaag
     1141 aatcctgcca tcaggcaaga gacgagcaaa aaatttccgg tgaatgtctc ccgttaaaca
     1201 aagtatcgct gtacctgaat aacgaaacac cggctggata acccgccagc catattaacg
     1261 ccataccaac ggattaaaaa tgccagcaat ggcagggatt cgttcaccct gaaatctgta
     1321 atgaggttaa aacacaatga ataaaatctt tatttgcgct gctattcctg acgaacaggc
     1381 catagaagaa gatagcgctg ttgcggtggc cactgccatt gaagccggtg atgagcgtcg
     1441 cgcacgcgca aaatttcact ggcaatttct ggagcaattc ccggcagctc aggactgcgc
     1501 ttataaattt attgtctgcg aggataaacc cggcataccc cgccctgccc tcgattcctg
     1561 ggataccgaa tatatgcagg aaaaccgctg ggatgaggag tctgcgtcct ttgtcccggt
     1621 cgaacccgaa tccgatccga tgaacatcac ttttgacaat ctggcccctg aagtacagaa
     1681 cgccgtcatg gttaagttcg acgcatgtga aaacatcact gttgatatgg tgattagcgc
     1741 tcaggaattg ttgcaggaag acatggcaac attcgacggc catatcgttg aagcgttgat
     1801 gaagatgcca gaagttaacg ccatgtatcc ggagcttaag ctgcatgcca tcgggtgggt
     1861 taagcataaa tgtaaacctg ctgctaaatg gcccgaaatt caggcagaga tgcgcatctg
     1921 gaaaaaacgt cgcgaaagtg aacgcaagga aaccgggaaa tacatatctg tcgttgatat
     1981 cgcccgttcc agagtaaacc aacagaacac tgaaaacgct gctgaaaaaa ccggggctgt
     2041 cactgttgcc gttcgccgcg aatacaaaca gacatggaaa acactcgaca atgaactggc
     2101 ctgcgccctc tggcccggtg atgtggatgc aggaaacatt gacggtacca tccatcgctg
     2161 ggcgacaaat gaggttatcg acaaggatcg cgaagactgg aagcgtatct cagcatcaat
     2221 gcgcaaacag cccgaagcac ttgcctatga ccgtcagact atttttggcc ttgttcgcga
     2281 acgtccgatc gatattcaca aagatcccgt tgcactgaac aaatacatca gcgaatacct
     2341 gacgacaaag ggcgtgtttg aacatgaaga aacagaccag agctctgctg atgctatcct
     2401 gtcgtcagca gcacaaactg atccagtgga gacggcggaa tccaattctc aaaaaaatga
     2461 aatcctggtg gaagctgaac catctgtaga gcgtgaagga ccgttttatt tcgtctttac
     2521 cgataaggac ggggaaaaat acggcagggc aaacaaactt tctggcctgg acaaggcgct
     2581 ggctgccggt ggtaccgaaa tctcaaaaga agaatatttt gcccgaaaaa atggcacata
     2641 tacgggctta cagcaaaata cagataccgc agaagattca gaacaaccag agccggtaaa
     2701 agttaccgct gacgaagtaa acaaaattat gcaggcagcc aatatcagcc agcctgacac
     2761 cgataaattg cttgctgcat cacgtggtga atttgttgaa gggattagcg acccgaatga
     2821 tccgaaatgg gttaagggga ttgaaacccg cgattttgag aaccagaacc agcccgaatc
     2881 ggaacaaaac gaccagaaag cggaacaaaa cagcccaaat gcgttacaaa acgagccaga
     2941 aacgaaacag cctgaaccag aagagcaaca agaaccggaa aaagtctgca ccgcctgcgg
     3001 tcagatcgga ggcggcaact gtcctgactg tggcgcggtg atgggcgacg caacatacca
     3061 ggaaacattc gatgaaaaaa atcgggctga agttcaggaa gatgatccgg aggaaatgga
     3121 aggcgctgaa catccgaaca atgagaatgc tggtaacgat cagcatcaca ccagcgatag
     3181 tgaaactgga gaggcggcag atcccttaat tgcggtgaac ggtcatcacg ttatcacatc
     3241 caccagcaga gtgtggattc acctgtcggt cgaccttgaa acgatgggaa caaatcctga
     3301 tgcgccaatc aactctattg gcggtacatt ttttgatccg gcaaccggag agatgggacc
     3361 tgaattcagt aaagctatcg atctggaaac ctccggcgga atcatcgacc gtaaaacaat
     3421 aaagtggtgg gcgaaacgat cacgcgaagc acaatccgct atttttaccg atgaaatctc
     3481 gttagatgtt gctctccggc tattcataga atttatcgaa aaaaactccg gcgggtgttt
     3541 tgtccaggta tggggaaatg gagccaactt cgacaacgta attttacgcc gttcatatga
     3601 acggcagggg atcccctgcc cgtggcttta ctacaacgat cgcgatgtac gcacaatcgt
     3661 tgaactgggt aatgccattg gctttgatgt cagaatggct attccattcg aaggcgtacc
     3721 ccacaatgcg ctggacgatg cccgtcacca ggcaaaacaa gtttcagcaa tctggcaaaa
     3781 actgatcccg agtcaggctg atttttaatg ttcaaccccg gtcgtcgtcc gcaagctata
     3841 gtggcggcgg ccatgattag cgaacaacgc tcatggcaag acttattctg ctcactgagt
     3901 gggcaaaaga ggaattcagt gaaccggtcc ctactccggg cacgttaagt aaatacgcta
     3961 aagccggaat gatatttcct ctccccaaaa aagttggaag acactggcga gtggatcctc
     4021 gagctcgctt tgtcggaatg gtaaacaagc cggaagtgat cgccacagat caccctgctt
     4081 tgaagaggat actggaagat ggcgcgccca cgaaaatata aaactgaagt tccgggatta
     4141 tctccgtatt ttgacaaaag aaataacaaa gtttactggc gttacaggca tcccataaca
     4201 ggaaaaaatc acgggctcgg cagtattgac cagaaacagg cagaagctat tgcagcagaa
     4261 gcgaacagcc gtcttgccag gcaacaaatg gaacaaatgg aacaaatgct cactctgcag
     4321 gagaaaatta ttcgtgatac cggtggttca tcaaccgttt ctgtttttct gaatagttac
     4381 cgaaaaattc aacaggaaag atatgaaaac ggagagatca aactcaacac actgaaacag
     4441 aaagcatccc ctctcagggt atttgatgaa cgttttggta ccagaccttt agatgccata
     4501 accgtaaaag atgtggtatc ggtgctggaa gattacaagg caaaaggaca taacagaatg
     4561 ggacaaattt tcaggaaagt actgattgat gttttccggg aggcccagca aacgggcgat
     4621 gtcccgcctg gctttaaccc tgcagaatcg gcaaaaaaac cacaggtacg gatatcacgg
     4681 caacgactga cctttgatga gtggatgatg atttataatg cagcggaaaa ggatggttac
     4741 tttttacagc gtggtatgct gctggcactg atgacaggcc agcgcctttc agatatttgc
     4801 aaaatgaaat tttcggatat cagggatggt tatcttcatg tcgaacagca aaaaacagga
     4861 acccggattg ccatccctct ggctctgcgt tgcgataaat taaatctcac ccaggatgat
     4921 gtggtgtcat cctgtcgcga ttgcgttctt agtccgtggc tattgcacca ccatcatgct
     4981 aaagggacag ctaagcgcgg cgggatggtt aagccagcaa cgttaaccgt tgcatttaaa
     5041 aaagcccggg attctgtgga ttacaactgg cgtgctaatg gcaccccacc ctctttccat
     5101 gagcagagat ctttatcaga acgattgttc agagaacagg gaattgatac caaaattttg
     5161 ctgggccatt cgaatcaaaa aatgaccgat atttacaacg acgcacgcgg taaggaatgg
     5221 aaaaaactgg tcatttgatg accagttttg cagaagagtt ttgcagaggt tttgcagagc
     5281 aaattaaaaa cggtaccctg cagagaacat aaacacccac ggatcaaggc gtacattgtc
     5341 gctataactg cctttagcca gtttacgacc atcagattta tacttagccg tggtatcgat
     5401 atccatgtac cacactgaca tgttaaccaa ccagtcacgg ttaatcagat aatcaacccc
     5461 cacctgcccg gcagctcccc aggaatcttt cagactgaga tcggaaagcc ctgcctcttt
     5521 gccatgatcg ttaaatccat tatcaaagaa ggtggtgtag ttaataccgg ccccaacgta
     5581 aggacggaat ttgctgctgg catcaccaaa ataccactgc gccatcagtg ttggcggcag
     5641 atgatgaacg gttgcaatat cgccggtcgc ccgggtgccg attttatggc ggaacggcgt
     5701 cgctgccagt aattccacac caatgttgtc ggtcgccata taagtaaacg tcaggcccag
     5761 ttgagtgtta ttggtcacgc tgaatccacc cagacttcct aacgtaccac cagcaccttc
     5821 tgttggacgt acggttgcag aacctgcacg cataaaaaat tcgcctgctt catgcgcaaa
     5881 ggcactgcca gagagaagag ttgttactgc caaagccgcc actgttaact ttttcatatc
     5941 cgctccgtcg ttatggttat aaaaacgtga cgaatatacc tacattggag taacaaatga
     6001 tcctacatag atcacattac atatgataat tcaccattta ttgatctgga ttaatttaga
     6061 agttgcattg agaatcctgg ttaacgtgat ttaaatcaat ttttaaatat tttcgctacg
     6121 caaatattaa ctcgttcgcc acttgccgcg gtttgcccac gcctgaaggc cagcaattac
     6181 ctccgcttta caaagatatg cttttccata caagacctga tgctgccacc cgaggcttaa
     6241 ccagggtaca attgcccgct aattaacacc tgcataaaac tcaaggagag tgcatgtcta
     6301 tcacggcgca gtccgtatac cgtgacaccg gaaatttctt ccgtaatcaa tttatgacca
     6361 ttctgttggt atcgttgcta tgtgcgttta tcacagtggt gttagggcat gttttctcac
     6421 ccagtgatgc acagcttgcg cagctcaatg acggcgtgcc cgttagcggc agtagtgggt
     6481 tgttcgacct ggttcagaat atgtcaccgg aacagcaaca aattttgctg caggcttcag
     6541 cggcgtccac tttttcagga ttaatcggta acgccattct cgccggaggc gtaatattaa
     6601 ttacccagct ggtgtctgcg ggtcagagag tcagtgcgct gcgagctatt ggtgccagtg
     6661 cgccgatatt gcccaagtta tttattctga tttttctgac taccctttta gtacagattg
     6721 gcatcatgct ggtggtcgtt ccgggaatta tcatggccat tttactggcc ctggcaccgg
     6781 tgatgctggt acaggacaaa atgggcattt ttgcctcgat gcgtagcagt atgcggctga
     6841 cttgggcgaa tatgcgtctg gtggcacccg cagtactgag ctggttgctg gcaaaaacac
     6901 tgttgctgct ttttgcctct tcttttgccg cattaacccc ggaaattggt gccgtactgg
     6961 cgaacacctt gagcaacctg atttctgcca tattgctcat ctatctgttc cgcctgtata
     7021 tgttgattcg ccaataacct taagcatttg attacggaat cgtaaaatga agcagtttct
     7081 tgatttttta ccgctggttg tctttttcgc gttttacaag atttatgaca tctatgcggc
     7141 tactgcggcg ctgatcgtcg ccacggcgat tgtgcttatc tatagctggg ttcgctttcg
     7201 taaggttgag aagatggccc tgatcacttt tgttctggtg gtcgtcttcg gtggcttgac
     7261 gctgttcttc cacaatgatg agtttataaa atggaaggtt actgtcattt atgccctgtt
     7321 tgcgggtgcc ctgttagtca gccaatgggt gatgaaaaag ccgctaattc agcggatgct
     7381 gggtaaagaa ctcacgctgc cgcaaccggt atggtcgaag ctgaatctgg cctgggctgt
     7441 tttctttatc ctttgcggtc tggcaaacat ctacatcgca ttctggctgc cgcaaaatat
     7501 ttgggtcaac tttaaagtct ttggcctgac cgcccttacc ttaatcttca cattgttaag
     7561 cggtatctat atctaccgcc acatgccgca ggaagataaa tcctaaccag actgccagtc
     7621 cttcggggct ggcgctgtat ctcctctgtt ctcctcagga aaatcatagt agcatcgcgc
     7681 ctgtgatttt ccttttaagt cggttttacc atgtctacaa cacataacgt ccctcagggc
     7741 gatcttgttt tacgtacttt agccatgccc gccgatacca atgccaatgg tgacatcttt
     7801 ggtggttggt taatgtcaca aatggatatt ggcggcgcta ttctggcaaa agaaattgcc
     7861 acggtcgcgt agtgactgtg cgggttgaag gaatgacttt cttacggccg gttgcggtcg
     7921 gcgatgtggt gtgctgctat gcacgctgtg tccagaaagg gacgacatcg gtcagcatta
     7981 atattgaagt gtgggtgaaa aaagtagcgt ctgaaccaat cgggcaacgc tataaagcga
     8041 cagaagcatt atttaagtat gtcgcggttg atcctgaagg aaaacctcgc gccttacctg
     8101 ttgagtaata gtcaaaagcc tccggtcgga ggcttttgac tttctgctta ctgaatttcg
     8161 gtggtgccgt taattttaaa caggatattc accacaatcc cactgcctgg cttacccggc
     8221 tcataacgcc atctgcgcat cgcatttttc acctcacgct caaacatgtt cgcaggcttg
     8281 gctgagagga tttgtacgtt atccacgcga ccatccggcg tgacgtcaaa tttaacttta
     8341 acctgccctt caatgcgcaa tgcctgtgct cgtgccggat actgcggctg attacggctt
     8401 aatgcgcgtg gtcctgaagc cacactggta accggcttgc tggttgcagc cgttgccgta
     8461 cttgatgtgg ggcgcgcggg tgccgtattt tcaaacggtg atgccggacg cgactctacg
     8521 ggtttgatat cgcgttttgg ctgctcctgt acctttttca ccggcttcgg ttttggctta
     8581 ggtttcggct tcggcttttc aatgaccacc ggtgcttctt ttggcggttc ggggatcggc
     8641 tcaggttccg gttctggctc taccaccggc tccggtggcg gctgaacggc ttgtggcggt
     8701 tcgagatcag caggcgcagc catcgtgaca gaaattggct gcgcaggcgc aggtagttca
     8761 ataacctgat gtaccgaggt atagagcaga cccgccacaa cagcaccatg aatgcagacc
     8821 gaaagtaacg tcggccaggg gaagcggcga ggtaaatcaa gggtcattgt attgtagcta
     8881 ttcggcattg tagcattagc cataacgttt ataagttcat ctttaaatga taatcaattg
     8941 catgggagtt tatcaccgtg agaaccgtaa taacatacct tcggttcagt tcagctatac
     9001 aaggcgctga aggtgctgat tcaaccagac gacaaaatga gctattcaaa cagtggttaa
     9061 ggaagaatag cgatgctcag gtagttgcgt ctttcagtga tgaagggttg agtggttaca
     9121 aaggtaagca ccttactggt cagtttggtg acatgttagc ccgtattgag gctggggagt
     9181 ttccagcagg tacaattttg ctagttgaga gtatcgaccg tataggccga ttagagcatc
     9241 ttgaaacaga agccttgatg aaccgcatct taggcaacgg gatagagatt cacacattgc
     9301 aagatggcct tatctacaca aaggatgctt tagccgatga ccttggcata agcatcatcc
     9361 agcgtgtcaa agcctacata gcgcatcagg aatctaagca gaaatctttc cgtgttagtc
     9421 agaagtggga acaacgtgca aaacttgccc ttgctggtga acaacgtctt acaaagatgg
     9481 ttcccggatg gattgaccct gatactttca agtgcaatga acacgctgag actgtaagac
     9541 tgattttcaa gctgctgcta gatggtgaaa gtctgcataa cattgcacgt cacctacaaa
     9601 gcaacgatat aaagtctttt agtcgccgta aagatgctaa cgggtttagt gttcattctg
     9661 tgcgcactat cttaaggtca gaaacaacga gaggtacact accagcatca cagcgcaatg
     9721 accgccccgc tataccgaac tactacgaag ccgctataga tgctgcaacg ttcaataagg
     9781 ctcaagagat tctcgacaag aatcgtaaag gccgcacacc tgcaagtga
//
LOCUS       DAZGAN020000156         9767 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGAN020000156 DAZGAN020000000
VERSION     DAZGAN020000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.156"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..338
                     /locus_tag="ACHKYO_RS22430"
                     /old_locus_tag="pgaptmp_004486"
                     /pseudo
     CDS             1..338
                     /locus_tag="ACHKYO_RS22430"
                     /old_locus_tag="pgaptmp_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004135114.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(599..1168)
                     /locus_tag="ACHKYO_RS22435"
                     /old_locus_tag="pgaptmp_004487"
     CDS             complement(599..1168)
                     /locus_tag="ACHKYO_RS22435"
                     /old_locus_tag="pgaptmp_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431210.1"
                     /translation="MFSPTILLTLAGGIIMLLFCLFSFLIDFKLTRPIVFMCGGVFIG
                     VGVLGHITFIEILEHCKELATSGNVSRDVLSRLERYHLIFTYILPFISGAIGTNVISD
                     ALLKHQTYERSFSFIQFVRDCAEIFLVLIGLVLGVFCIFFFGVSILVAFTRNLVFLYF
                     PRIQRWVYLKVLKISMLMKNVFRSKPKRK"
     gene            complement(1952..2179)
                     /locus_tag="ACHKYO_RS22440"
                     /old_locus_tag="pgaptmp_004488"
     CDS             complement(1952..2179)
                     /locus_tag="ACHKYO_RS22440"
                     /old_locus_tag="pgaptmp_004488"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431211.1"
                     /translation="MTEDDVTPEAQLRFDMMVDQVSVSLTEPFRAQHRQRHPKMLSTT
                     DAKSTNSAKVAAASLTDSENAAAAVKNQKAT"
     gene            complement(2573..2854)
                     /locus_tag="ACHKYO_RS22445"
                     /old_locus_tag="pgaptmp_004489"
                     /pseudo
     CDS             complement(2573..2854)
                     /locus_tag="ACHKYO_RS22445"
                     /old_locus_tag="pgaptmp_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514848.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2907..4120
                     /locus_tag="ACHKYO_RS22450"
                     /old_locus_tag="pgaptmp_004490"
     CDS             join(2907..3198,3198..4120)
                     /locus_tag="ACHKYO_RS22450"
                     /old_locus_tag="pgaptmp_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4431212.1"
                     /translation="MTKNTRFSPEVRQQAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPLEKMMPLLDKLREQYGGGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLK
                     KEIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVS
                     RKAVAACNRVNRQFVAERPDQLWVADFTWVSTWQGFVYVAFIIDVFAGYIVGWQVSSS
                     METTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAGVIHRKSWKNRTEVELATLTWVDWYNNRRLLERLGHIPPAEAEKA
                     YYASIGNNDLAA"
     misc_feature    3188..3304
                     /locus_tag="ACHKYO_RS22450"
                     /old_locus_tag="pgaptmp_004490"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(4160..4519)
                     /locus_tag="ACHKYO_RS22455"
                     /old_locus_tag="pgaptmp_004491"
     CDS             complement(4160..4519)
                     /locus_tag="ACHKYO_RS22455"
                     /old_locus_tag="pgaptmp_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431213.1"
                     /translation="MKTQLKLELNMKKTLLARVVKGIAVCGLAMSSISYAATSVNAVH
                     NVVEESVWSIDYVDSGNTKAGQQDVSQPWGKLVVTLESGSPAIPSIHLPEGVRKGFDS
                     QFEWTHTESGVKYLGVI"
     gene            complement(5152..5535)
                     /gene="afaD"
                     /locus_tag="ACHKYO_RS22460"
                     /old_locus_tag="pgaptmp_004492"
     CDS             complement(5152..5535)
                     /gene="afaD"
                     /locus_tag="ACHKYO_RS22460"
                     /old_locus_tag="pgaptmp_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AfaD family invasin"
                     /protein_id="HGU4431214.1"
                     /translation="MVSGGHAQAEELNIVPHKGMTGELRDGMKIATGRIICREAHTGF
                     HIRINARQQESRPGHYIIQGRRDSRHELRVRIAGEGWSPSVTGGQEGMVSYGQREQGV
                     FDVVADGSQRVEADEYIYSVTGECL"
     gene            5593..6805
                     /locus_tag="ACHKYO_RS22465"
                     /old_locus_tag="pgaptmp_004493"
                     /pseudo
     CDS             5593..6805
                     /locus_tag="ACHKYO_RS22465"
                     /old_locus_tag="pgaptmp_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6813..6887)
                     /locus_tag="ACHKYO_RS22470"
                     /old_locus_tag="pgaptmp_004494"
                     /pseudo
     CDS             complement(6813..6887)
                     /locus_tag="ACHKYO_RS22470"
                     /old_locus_tag="pgaptmp_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6982..7605)
                     /locus_tag="ACHKYO_RS22475"
                     /old_locus_tag="pgaptmp_004495"
     CDS             complement(6982..7605)
                     /locus_tag="ACHKYO_RS22475"
                     /old_locus_tag="pgaptmp_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4431215.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGLSQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTFRYHLSHRIRRMVGNENTSSPYQQIAREVKVPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(8124..8351)
                     /locus_tag="ACHKYO_RS22480"
                     /old_locus_tag="pgaptmp_004496"
     CDS             complement(8124..8351)
                     /locus_tag="ACHKYO_RS22480"
                     /old_locus_tag="pgaptmp_004496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431216.1"
                     /translation="MARPAGSLNKRTISTNTTTPGEVKSFSLEHFLKKWRRTKMGRHT
                     WKVSPQTESQEQKQCAGAKDRELTGNSTAAE"
     gene            8675..9178
                     /locus_tag="ACHKYO_RS22485"
                     /old_locus_tag="pgaptmp_004497"
     CDS             8675..9178
                     /locus_tag="ACHKYO_RS22485"
                     /old_locus_tag="pgaptmp_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HGU4431217.1"
                     /translation="MVTLTINRKPQGIFSKSPATPLQDKTNTVHNEKPLRVRNQTPWR
                     HMTKRQRKNRKRINRLVEHWPELFNREKTQPLKVEIPDDLIQDIAIRELAFGAGALRA
                     AVASYVQSPRYYRALMAGGARYDLKGQPCGEVTPQEQKEAETRLMVLNDRQKPENLVD
                     YTTALIH"
     gene            complement(9172..9720)
                     /locus_tag="ACHKYO_RS22490"
                     /old_locus_tag="pgaptmp_004498"
     CDS             complement(9172..9720)
                     /locus_tag="ACHKYO_RS22490"
                     /old_locus_tag="pgaptmp_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431218.1"
                     /translation="MQITEALISEPGDIRRFVQQAVDHWPHLLAFYFTLHSAEGNING
                     QQIHAFCTSFYRQVHERITERNHMASPASPLVLRWLREQHGGATIRCLLLLSQTSICH
                     PRASVTVDEQCSQVVDLLQHSWQVISAGGQCRVERCFRVARGDTSGQYVALKTAALSL
                     GLPVVTAITHRPVQRCTLITAQ"
BASE COUNT         2605 a         2504 c         2161 g         2497 t
ORIGIN      
        1 ggaataattc gcgtcaacat catccctgct ctgctctatc ttggcgtgaa ggggaagata
       61 aacagaagca tccctatcag taaagagctt tatgaagaga gaatagcgct cgatgggttc
      121 aatttcttca cagactgcta ttttcaatct ttatccgtga tggaaaaaac gtctatcgtg
      181 ttccctcgag gtcaactgac acacgttcta cgccatacgt ttgcggcgta cttaatgatg
      241 ttgagtggaa acaaccaggc cttacaaaaa ttctcggaaa ccacgatata aaaatgacta
      301 tgcgttatga gcatctggta ccgaatcatc tggaaacata gctccgtttc aatccactgg
      361 caacgttacc aaatggcgac aatgtggcat taccccgtaa taaccaccac taaccaccac
      421 taaccaccac taaccaccac attaagttat cgtttttaaa aaactcattg tttttattaa
      481 accttttaga taacacatca tccattctta cttgactcag gtttcaaacc caaaaatcaa
      541 gtaaaaatta accatccata atcagcgaaa tgaataatga aaatcaatta attaagtatc
      601 atttgcgctt aggttttgac ctaaacacat tcttcataag catactgatt tttagaactt
      661 tcaaataaac ccagcgttga attcttggaa aatacaaaaa aacaaggttc cgcgtgaatg
      721 caactaatat agacacgcca aagaaaaata tacaaaaaac gcccaaaacc agcccgataa
      781 gaactaaaaa aatctccgca caatctctga caaactgaat aaaagagaaa gaacgctcat
      841 aagtctgatg ttttaataaa gcatctgaaa taacattggt accaattgca cctgatatga
      901 aaggaagaat ataggtaaat attaagtggt aacgctcaag gcgcgatagt acatcacgcg
      961 atacattacc agatgtagcc aattctttgc aatgttctag aatttctata aaagtaatgt
     1021 gacctagaac accaacacca ataaagacgc ccccacacat aaacacgatc gggcgtgtca
     1081 acttaaagtc gatcagaaaa gaaaatagac agaataaaag cataattatg ccaccagcaa
     1141 gagtaagcaa gatagtcgga gagaacataa aatcgaaatt catgggtata tatctaactg
     1201 tgatcgtatt cttttcttga ttaaaacatt gtgtgtccta aagatgttaa tgtcaatgga
     1261 gtataatcaa tggcctatat tcttgttgtt gacaaaaatt catctgatga atgatcgcct
     1321 caaaaaatac agagcctgtt taagattctg tgtagatgtc ttttctcaga agtgaccgtc
     1381 cgggcggtca ccgaactcgg tgataaagcc agtccttcaa cgacatcgtc catttctaga
     1441 gcgaacttca gtttgaaaaa gtgggcacct acgttaccaa tactggctta atagctacat
     1501 acggctgtca gtttactctt atgtatgttt cttgcctgga ctaaaacaac aaaatttgaa
     1561 cgactttcaa taacagaaaa gtcctgtaca tttgtcccaa ccatttacga cgtataaacc
     1621 gtacactgag ttttagtaag caattcagtg cctttcccgg aggctacagt atcgccttta
     1681 ggcccctgtt gacctgcatc cctttttgcc tcttgcggcc ccgtaacacc tttcggtccc
     1741 tctgggcttt ccagaactta aatacccttc accaaatttt cagccttggc cgctgtggat
     1801 tctgcagcgt tacatctttt ttagtctccg tcgcctgcca catctcctca tattttgagg
     1861 cttcaggaga tgatgctaat gacaccacct gacaggctga ctgtagcctt tgcgtttatt
     1921 tcgctgttcc tgacgaatgc gtcactcgtt tttacgttgc tttctgattt ttaactgctg
     1981 ctgcggcatt ttctgagtct gtcagtgagg cagccgccac tttggcgctg tttgttgact
     2041 ttgcatctgt cgttgacagc attttcggat gtctttgcct atgctgcgct ctgaaaggct
     2101 ccgtcagcga caccgacacc tgatccacca tcatatcaaa tcggagctgc gcttccggag
     2161 taacatcatc ttcagtcatc gtccctacag ggtccccgcc tctaaaacct tgagcaaata
     2221 gaacaacaca ctactggtca tactccacat ccatgctgta acgtccagat ttatcagaat
     2281 tttctgagcc accgagttta ccaccacctc ggtggtgtgg tacgttttac tttcagtcct
     2341 atctggcttt ttgtaccccc catgccatct ttatgcacac cagaaatttt aactgtaata
     2401 cctttccacc aataaaaaag cccgcagcaa taatgccacg ggctcaggga cagtagaact
     2461 ttacgcttcc tcaaacgcag ttcaccgcat caggcgaatg aacatgtgta ttatagcaat
     2521 atttaaagga gataaagtgc catcttacgt cagaaacggt attcgtgcag gtcgacaaca
     2581 cattaaatgt cagggagata tgacagtgtc ccgccggcat cgcaatcagc gatgagccct
     2641 cctactgaca tccattgccg ttttctgaaa aatccccacg ttttcagcac ctgaagatta
     2701 caggtttaat accagtacca ctgctgattc gagttattga aattctgctg tttcgtggga
     2761 cgaaacaaca caaggggaat gataatctgc cggcctcccc ctgaacatca taaacagtaa
     2821 cagttaccgt gccactggca taactgctat tctgaaccgc cccggaaatc ctggagacta
     2881 aactccctga gaaagaggta aacaggatga ctaaaaatac tcgtttttcc cccgaagtcc
     2941 gtcagcaggc gattcgtatg gttctggaaa gtcaggatga atatgactca cagtgggcgg
     3001 caatttgttc cattgcccca aagattggct gtacgccgga gactctgcgt gtctgggttc
     3061 gccagcatga gcgggatacc gggggcggtg atggtgggct caccagcgct gaacgtcagc
     3121 gtctgaaaga gctggaacgt gaaaatcgtg aactgcgccg cagtaacgat atccttcgcc
     3181 aggcttccgc ttattttgcg aaggcggagt tcgaccgcct ctggaaaaaa tgatgccact
     3241 gctggataag ctgcgtgagc agtacggggg cggaccggta tgcagcgaac tgcatattgc
     3301 cccgtcaacg tattaccatt gtcagcaaca gcgacatcat ccggataaac gcagtgcccg
     3361 tgcgcagcac gatgactggc tgaagaaaga gatacagcgc gtatacgatg aaaatcacca
     3421 ggtatacggt gtgcgtaaag tctggcgtca gttgttacgg gaaggtatca gagtggccag
     3481 atgcactgtg gcacgtctca tggcagttat gggacttgcc ggtgttctcc ggggtaaaaa
     3541 ggtccgcact accgtcagcc ggaaagccgt tgccgcatgc aaccgcgtaa accgtcagtt
     3601 cgtggcagaa cgtccagacc agttgtgggt ggctgatttt acctgggtaa gcacatggca
     3661 gggcttcgtt tatgtggcgt tcatcattga tgtgttcgcc ggatacatcg tgggatggca
     3721 ggtctcatca tccatggaaa caacattcgt gctggatgca ctggagcagg cgttgtgggc
     3781 ccgtcggccg tccggcacaa tccatcacag tgataaaggt tctcagtatg tatcgctggc
     3841 ctacacgcag cggcttaagg aagccggatt actggcatca acagggagta ctggcgactc
     3901 gtatgacaac gcgatggcgg agagcatcaa tggtctttac aaagcggggg taatacaccg
     3961 taagagctgg aaaaaccgga cagaagtgga actggccaca ctaacgtggg tggactggta
     4021 taacaatcga cgattgctgg aaaggctggg ccatattcct ccggcagaag cagaaaaagc
     4081 ttattatgct tccatcggaa ataatgatct ggcagcctga gttcacagat aaaatactct
     4141 ccaggaaacc cggggcggtt catattactc ctaagtattt aacgcctgac tcggtgtggg
     4201 tccactcaaa ctggctatca aaaccctttc tgacaccttc tggcaggtga atactgggga
     4261 ttgcaggtga tccgctctca agtgttacaa ccagctttcc ccaaggttgg gaaacatctt
     4321 gctgaccagc tttagtatta ccgctatcaa catagtcaat cgaccagact gactcctcta
     4381 caacgttgtg gactgcatta acactggtag ctgcatagct gatactactc attgccagac
     4441 cacagactgc aatacctttg accacacgag ccagtaatgt ttttttcatg tttaactcca
     4501 gtttcagttg agttttcatc agatgtaatc cagccacttt ccgttttctc tctctcagac
     4561 atccagtaag ccttcttacc ctcaataacc accgttcccg gtgatgattt cattcacgtc
     4621 cgccacaacg gtggccccgt gttcatcctt cccgcttccg ggaaacacca ttctgtctga
     4681 acctttcccg gagaatgtct tgtccggata tctcctgaac tttccccagt aaaaatattc
     4741 actgcgtcgt gattctgttt cttctcataa gaaaaataaa accgtgtcca tataacggac
     4801 ataataagca tctcccgtag atttaaatca gcatcagcct gcttaaattc atttccattt
     4861 atccggcacc aggtttaata tacactctgg tgacattccc gtttccggag tttatattct
     4921 tccggcaatg caaaggatat tataaatata atataactct ttcttcatct gttatctgcg
     4981 tgtgccactg acttttcttt aatgcaatat ctgcaattac cttcaacaca aaccttcagt
     5041 accttgtgtt ttattctcct gttctctgac ctgttattct ttatatatct cattgcgata
     5101 tgttgttatt aatactgtta ttaataccct gtctgttcat tcacgctccc gtcacaggca
     5161 ttctcccgtg acggagtaaa tatattcatc cgcctccaca cgctggctgc catcagccac
     5221 cacatcaaac acgccctgtt ccctctgacc ataactgacc attccctcct gaccacctgt
     5281 cactgacggt gaccagccct ctccggcaat cctgacgcgc agttcatggc ggctgtccct
     5341 tctcccctgt atgatatagt gccccggacg gctctcctgc tgacgggcat ttatccggat
     5401 atgaaaaccg gtatgcgcct cacggcagat aatccggccg gtcgctatct tcattccatc
     5461 cctcagctca cctgtcattc ccttatgcgg aacaatattc agctcttctg cctgagcgtg
     5521 accaccgctg accatcagta ccactccggt taatactgta gtggcacagt aaatttggcc
     5581 acctgattaa aggtgatatt ctcacctcaa cacaaaacag gtgacttatg aacaagaaaa
     5641 ccaaacgtac tttcacccct gagttcaggc tggaatgtgc acagctgatt gttgataagg
     5701 gctattcgta tcgacaagcc agtgaggcga tgaatgtcgg ttctaccacg cttgagagct
     5761 gggtacgtca gctcaggcga gagcgccaag gtattgcgcc ctctgcaaca cccattaccc
     5821 ctgaacagca acgtattcgc gagctggaaa agcaggttcg ccgactggag gaacaaaata
     5881 cgatattaaa aaaggctacc gcgctcttga tgtccgactc actgaacggt tttcgatagt
     5941 ttccagactg agtgacagcc actcagttgt cagcctctgt tccgcactgg gaatacaccg
     6001 cagcagttac cggtactggc gaaaacgacg cgatacggtt aattcggctc gcgtcaggtt
     6061 gtgcagcgaa atacgccggg catggaacca gagccggggc tctgcaggcg cgcgcacgct
     6121 ggctgaaatg ctgacccaaa acggcgtccc gatgagccgt tatcgtgccg gacggctgat
     6181 gaaatacctg aacctgagca gttgtcagcc cggaaaacat caatacaaaa atgcctgtca
     6241 ggagcatacg tgtttgccga atctgctcga gcgtcagttc gctgtaccgg agccagacag
     6301 ggtatggtgc ggagatatta cgtatatctg ggcaggaaat cgctggtgct atctggcggt
     6361 cgtcatggat ctttttgccc gcagggttat cggctggagc ctgtcagcga atgccgatac
     6421 tgcactgata agcagtgcct tgcggatggc gtatgaggtg cgtggtcagc cgcgggacgt
     6481 catgttccat agcgaccagg gaagtcagta tacaggactg aaatatcaac agcttctctg
     6541 gcgttacagg ataaagcaaa gtgtcagccg acggggaaac tgctgggata acagtcctat
     6601 ggaacgcttc ttccgcagtc tgaaaacaga atgggtgccg acggatggtt acgtaggcaa
     6661 ggacgaggcc cggcaacaaa ttagcggtta tatcctgaat tattacaata gcgtcaggcc
     6721 tcaccattac aacggtgggt tgacgccgga agaatcagag aacagatacc gtttttactg
     6781 taaaaccgtg gccaatatta cttgaccact acacctctct ctgccagcca ctcattcagt
     6841 tcatgtgcca gagacagatt gataaaatcg taatcggacc ttgccattcc cgtttcctta
     6901 acacatgtct tcagactcag ggataaagta taaaaatgtc gccctgcagt tgccagagca
     6961 atattccccc tgatggttgt atcagagatg gtatgccggt actttgcggg ttgttcctgt
     7021 caggttatcg tgcacctcca cggtcgtggt caccaccggc attccggcct ttctcagcct
     7081 cagaacatca gccgcaatac gctgactgcc agaccagaac aggccgtcca gtgcagtcac
     7141 cagtaaaccc gcctccagtg catacttcag ccgttcacgg ggcactttca cttcccgggc
     7201 aatctgctga tatggcgatg atgtattttc attcccaacc atccggcgaa tacgatgaga
     7261 cagatgatac cggaatgtat ccggtacacc agaaagactg gccttcaggc tgtacacgca
     7321 gccaaaccgt ttatcgttga acaccacatt tttctggctg aggccccatt cttcccgaag
     7381 cgcggcaatc agttgcggcg tgcgggttaa aagcagccga aaaggcagtt caaaactggt
     7441 gacgtaatcc acattcagca gggcaatacg cagccgttct tctggtccgg cctctgtctg
     7501 ccggcactcc tccagaacat cctgccactg ctggcgaaga cggggcgatt cgttccgttc
     7561 tgtaaaacag tatttatccg ccagataatc aattcgtgta cgcatactgc aaagaattcc
     7621 gtataaaaat tcatctgaca aaactttatc agtccataaa aatcaacgga agcacagatg
     7681 attcactcgt ccattaccaa cggattttgc caataaccct ggcgttgaca acgggtaaca
     7741 taaaagacaa agactgaaca actgttttta acggcgatac tgcatgattt catcgccatc
     7801 cgtcaccgtc atatccacca gatgaaacaa ctctgtgcag gttttatccg caagttccgg
     7861 attatcaaaa cacgtcggtc agttcatgtt gtacggggac aggctgcgct ccccatgaca
     7921 gaacattatc gatatcacca gcatacgtgc cactcaaagt gttgcggcat cgtacggcgt
     7981 ggtcgtgttc ttctcttttt cctgttcaca ataaccagct taacggtcag aaggcatttt
     8041 cacccggatt atattcacct gcacaaccat gttcagccat gaccgctgat aagtgaacat
     8101 ccggagtata catggcataa agattattct gccgctgttg aattgccggt aagctcccgg
     8161 tcctttgctc cggcacactg tttttgttcc tggctctctg tctgaggtga aaccttccag
     8221 gtatgtcgcc ccatttttgt tcgtctccat ttttttaaga agtgctcaag tgaaaaactt
     8281 tttacctctc cgggagttgt cgtatttgtt gatattgttc gtttgtttaa agaacctgct
     8341 ggtcttgcca ttaatattac ctgctgattg cttcacgccg ggctcctcgc actgtgcgcg
     8401 ggttattgtt tcgttttcag tcccgggggc gccaggactc tgtttgcaga ccataacggc
     8461 ctgacctgta ctgtttcttc ggctctttga tgcccgttta cgccatttgt tccctgcccc
     8521 tctgtctgat aacgaatacg ctcactacgc acagcactga acaataccgg caacagcatg
     8581 tcttacatga cattcgctgt gccatccacc ggaaaacatt ttcagatacc ggatgaggta
     8641 ccactgccat tgacagcacg aggtaacaat actgatggta acgctgacca ttaaccgtaa
     8701 accgcagggt attttcagta agtcaccggc aacgccgctg caggataaaa cgaacacagt
     8761 acacaacgag aagcctcttc gggtgaggaa tcaaacacca tggcgacata tgacaaaacg
     8821 ccagcgcaaa aaccgtaagc gtattaatcg tcttgtggaa cactggcctg agttattcaa
     8881 ccgggaaaaa acccaaccgc ttaaagttga gatacctgac gacctgatac aggatatcgc
     8941 catcagagaa ctggcatttg gcgcaggtgc attacgcgcg gcagtagcgt cttatgtgca
     9001 gtctccgcgc tattaccgtg cgttaatggc tggcggcgcc cgttacgacc tgaaggggca
     9061 gccgtgcggg gaagtgactc cacaggaaca gaaagaggca gagacccggc taatggtgct
     9121 gaatgaccga caaaagccag aaaacctcgt tgactacaca actgcgctga ttcactgagc
     9181 tgtaatcaat gtacagcgct gtaccggacg atgggtaatg gcggtcacaa ccggtaaccc
     9241 cagagacaat gcggcagttt ttaacgctac atactgaccg gatgtatcac cccgggcaac
     9301 ccgaaaacac ctttccaccc ggcattgtcc gccagcactt atcacctgcc agctatgttg
     9361 cagtaaatcc accacttgcg aacattgttc atcaactgtg acactggctc gcgggtgaca
     9421 aatactcgtc tggctgagca gcaacaggca tcgaattgtt gctcctccat gttgttcccg
     9481 caaccagcgt aataccagcg gcgatgccgg actggccatg tgattacgct cagtaatacg
     9541 ttcgtgcacc tgtcggtaaa aggaagtaca gaatgcatga atctgttgcc cgttgatatt
     9601 tccttctgcc gaatggagtg tgaagtaaaa tgccagcaaa tgcggccagt ggtccaccgc
     9661 ctgctgaaca aaacgccgga tgtctcccgg ctctgaaatt aaggcttcgg ttatttgcac
     9721 gaaatgttcc ttttcaaacc ctgttggatt ttgtcaagcc ggtacag
//
LOCUS       DAZGAN020000157         9684 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGAN020000157 DAZGAN020000000
VERSION     DAZGAN020000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.157"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(401..3073)
                     /gene="rcsD"
                     /locus_tag="ACHKYO_RS22495"
                     /old_locus_tag="pgaptmp_004499"
     CDS             complement(401..3073)
                     /gene="rcsD"
                     /locus_tag="ACHKYO_RS22495"
                     /old_locus_tag="pgaptmp_004499"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HGU4431219.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            3812..4915
                     /gene="ompC"
                     /locus_tag="ACHKYO_RS22500"
                     /old_locus_tag="pgaptmp_004500"
     CDS             3812..4915
                     /gene="ompC"
                     /locus_tag="ACHKYO_RS22500"
                     /old_locus_tag="pgaptmp_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU4431220.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSARGEGMTNNGRDALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNTAAYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIGTRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            5027..6082
                     /gene="apbE"
                     /locus_tag="ACHKYO_RS22505"
                     /old_locus_tag="pgaptmp_004501"
     CDS             5027..6082
                     /gene="apbE"
                     /locus_tag="ACHKYO_RS22505"
                     /old_locus_tag="pgaptmp_004501"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HGU4431221.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGKPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     IGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPKKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            6156..7220
                     /gene="ada"
                     /locus_tag="ACHKYO_RS22510"
                     /old_locus_tag="pgaptmp_004502"
     CDS             6156..7220
                     /gene="ada"
                     /locus_tag="ACHKYO_RS22510"
                     /old_locus_tag="pgaptmp_004502"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HGU4431222.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFHPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            7220..7870
                     /gene="alkB"
                     /locus_tag="ACHKYO_RS22515"
                     /old_locus_tag="pgaptmp_004503"
     CDS             7220..7870
                     /gene="alkB"
                     /locus_tag="ACHKYO_RS22515"
                     /old_locus_tag="pgaptmp_004503"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HGU4431223.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINNVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGELRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            7946..9589
                     /gene="yojI"
                     /locus_tag="ACHKYO_RS22520"
                     /old_locus_tag="pgaptmp_004504"
     CDS             7946..9589
                     /gene="yojI"
                     /locus_tag="ACHKYO_RS22520"
                     /old_locus_tag="pgaptmp_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HGU4431224.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         2390 a         2357 c         2483 g         2454 t
ORIGIN      
        1 gtggtgtcta ccagagtgtc agatatgcag gaaaatatga gctccgacag aaatgcagta
       61 aaaaaagaga gtctcgtaca cccctccaga aaactccagg gctgtaagtt gtattaaaaa
      121 gctgattttt ctttagcgat acagacctca ataagtataa cacccttcag ttactgtggt
      181 tatgtacagg tgttattttt aactgtgtgg ataaacagtg tcgattattc agattggtta
      241 tggggcatgt atgggacatt tttaatcggc gaaatacgtc gaagttcgtc gatatggtaa
      301 acgaatcatc tatccaaccc ttgaaaaacg gcgctcctgg acgatcttcg tcgattttta
      361 aaatgcaaca agtacgttgt tcatgtaata ggctaccttg ctacagcaag ctcttgacat
      421 aactgtcaat gtcgctgatg tatttttcta ttcctggaac atccttctca cgaatcagat
      481 gttccagcgt ttcacataac tgcttgccgg gtaccagatt tagcatggca aatacgcctt
      541 taagacgatg agccgtttgg gctaacgcag caaagtcact ggttgctgct tcagtataca
      601 gcctcttaac atcatccggt actgtgtcta caaagagcgc ataatagccg ctggcatgga
      661 gttgcgcatt ttcatctccg cccagaggcg attctgtcac ctcttcctgc gccagttgca
      721 cttcaattaa ttgtaagacc gcttcctgca tagcgttgct catattgaag ttgacgcaca
      781 attgaccagg cccaatttcc cgtacgccag actcatcatc gcttaaaagc aagccagagg
      841 cagtaagatt agacggatta tccgttaaaa agatatcata atcttgacta attaatcttt
      901 catcgggtgt gatacaggtt gcaccccaat tttctaactg gcgagtgaca atattccgaa
      961 tttctgccga agtaacatcc accattacgc agacatcatc cagtaaacgc tcttcttcct
     1021 cttcaacttc cgggtcagct gcgagcattt tgatatgcac agagtagcgt gtaccaagcc
     1081 catcccgcgt tttgatgttt aaatgaccgc ccagtttacg tgccagttga tcgctcagcc
     1141 agaatgccag cgggtccgcc ttgccatagc gatcgttttg ggtctggttg ataaacggga
     1201 agtgcaaatt atccatttca tgaatactta cgccttctcc cgtgtccaga atgcggaacg
     1261 tcaggcggtc ttcggaggac tcatcctgat caacctcaag ggtgattttt cccaattgcg
     1321 ttgaggtcac ggcatattgc atcagtagca gcaaaatacg tcgtaaggca tcgcgatcgc
     1381 cgcggcgcat atcgtgtgct ttcagatgat tgttaatcag cagttgcaga cctttacgct
     1441 tgatggcagg caacactgaa ggcacaactt catcaattaa atcctgcacg gagaacagca
     1501 ccgtctcact tttccagcta tcatccgcaa gcatgttcgc taactgtatt tcatcaacca
     1561 gccgcaccag cacatctgcc tgattcgcca gttgtttgct ttccggggcg ttgagtttag
     1621 ccgcgctctc cgccagggac tgtgcgggtt ctttcagcgc atcgccaatg tttttcataa
     1681 aggtcatccg cccctgctgg tttttctcat acagacgctg cgcctgcttg agtttcttgt
     1741 ttaccagcac ttcgcgatcc tgatcgcgaa taatgaaaat ttgtgtgcgc ggcgcgacct
     1801 ggctgcggaa catgcggatc tcatacagct cgttattgat cgtcgcctga ataatcccct
     1861 gatgctgttc cgccatggtg gtgatgtttt gcagattcaa atgcggcagc aaatgatcgg
     1921 caattttgtt acttatgaca gtgcggttcg attcctgatc gtgaaccagc aggccgagcg
     1981 gcagcagtga gactatctct tcattaattg cccgtaaaat gcgcaattcg ttattgaccg
     2041 ccgtgctggg tagactttct gtactgcggc tggagaaatg gcggaatgtg gtatagccaa
     2101 ataacgccag cgccagcaaa ccgatgttca gcagcagtgg cagcagaatg ttttgcaacg
     2161 tatccagcaa taaggtgcca taaggaacct gccagaccag gcgcatatcg gtagagttga
     2221 gtgccgagga gatttcaatc ttcgtactgt taaagtggat actgacgcta tccgtccctt
     2281 ctttctcatt atcattgttt cccgttgccg tcgcgtctgg ctcaaggcgg aaactgtcca
     2341 gcggcatacc cggtgggatc aaatcattaa tcggcagatc aaaagccacg accgttgcca
     2401 gatgtcctgg ctggttaaag gtagtacgca aggtaaagta atgaccgttc tgccaggcca
     2461 ggcggcgcat gttagaaaag ctttcgcgtt catcgagggc gttggcctgt tgcaacatct
     2521 ctgcacgacg tgaatcaaca atgtcgctga cggtcgattc tttaaatccg gaggtgagat
     2581 ctttaagggg cagggttgag atcagcacca gactgttatc ctgaccattc aggtaataca
     2641 tcgaccacgg tacattttct gcgccccaca atgtgtccag ataggtggac atccgctgag
     2701 tcatctcaag cgttgagttg tcgtgagagc caaagattaa cgcttccgtt ttgcggcgcg
     2761 gtttctccag atagtagaca tcctgtttca ggcgcgtctc ttgtaaacct tcgccggagg
     2821 agggggaggt cgtcgcggca atgttgtcgt agatctgcca ggtcacgtaa cgccaggtat
     2881 cgacgcgctt ttggatagcg tgggtaatgt cgacaatctg gtaactttta tctttcagcc
     2941 atgcgttaac ggcgctttgt accattacac ccatcgtcac cagtaacaca atgatcaaca
     3001 gtaaaaagaa gcgggtaatg ctccccggta ggagtgaaaa gcgggtcgtg gccgttgtct
     3061 ctttctgacg cattcgtgtt tatgacctgt taaaacttcg cgaagtgata gggcagtata
     3121 aagggtacag tgtgaaattc cagactctta cctgattgat gattctctat tctcccgtga
     3181 tatttatcat cctgcattgg gggaaataga tgtacaagcg ccattttgct gtacaaataa
     3241 cctacaaaaa gcccaacgaa aataattgca aataaacaaa gattgctgga aattatgctg
     3301 atgttaatta tttgtgaaat agttaacaag cgttatagtt tttctgtggt agcaaagaat
     3361 aatgaaaagt gtgtaaagaa gggtaaaaaa aaccgaatgc gaggcatccg gttgaaatag
     3421 gggtaaacag acattcagaa atgaatgacg gtaataaata aagttaatga tgatagcggg
     3481 agttattcta gttgcgagtg aaggttttgt tttgacattc agtgctgtca aatacttaag
     3541 aataagttat tgattttaac cttgaattat tattgcttga tgttaggtgc ttatttcgcc
     3601 attccgcaat aatcttaaaa agttcccttg catttacatt ttgaaacatc tatagcgata
     3661 aatgaaacat cttaaaagtt ttagtatcat attcgtgttg gattattctg catttttggg
     3721 gagaatggac ttgccgactg attaatgagg gttaatcagt atgcagtggc ataaaaaagc
     3781 aaataaaggc atataacaga gggttaataa catgaaagtt aaagtactgt ccctcctggt
     3841 cccagctctg ctggtagcag gcgcagcaaa cgctgctgaa gtttacaaca aagacggcaa
     3901 caaattagat ctgtacggta aagtagacgg cctgcactat ttctctgacg acaagtctgt
     3961 agatggcgac cagacctaca tgcgtcttgg cttcaaaggt gaaactcagg ttactgacca
     4021 gctgaccggt tacggccagt gggaatatca gattcagggc aacagcgctg aaaacgaaaa
     4081 caactcctgg acccgtgtgg cattcgcagg tctgaaattc caggatgtag gttctttcga
     4141 ctacggtcgt aactacggcg ttgtttacga cgtaacttcc tggaccgacg tactgccaga
     4201 attcggtggt gacacctacg gttctgacaa cttcatgcag cagcgtggta acggctttgc
     4261 gacctaccgt aacactgact tcttcggtct ggttgacggc ctgaactttg ctgttcagta
     4321 ccagggcaaa aacggtagcg ctagaggcga aggcatgacc aacaatggtc gcgatgctct
     4381 gcgtcagaac ggcgacggcg ttggcggttc tatcacttat gattacgaag gcttcggtat
     4441 cggtgctgca gtttccagct ccaaacgtac tgatgctcag aacaccgctg cttacatcgg
     4501 taacggcgac cgtgctgaaa cctacactgg tggtctgaaa tacgatgcta acaacatcta
     4561 cctggctgct cagtacaccc agacctacaa cgcaactcgc gtaggttccc tgggttgggc
     4621 gaacaaagca cagaacttcg aagctgttgc tcagtaccag ttcgacttcg gtctgcgccc
     4681 gtctgtagca tacctgcagt ctaaaggtaa aaacctgggc accatcggta ctcgtaacta
     4741 cgacgacgaa gatatcctga aatatgttga tgttggtgcg acctactact tcaacaaaaa
     4801 catgtccacc tacgttgact acaaaatcaa cctgctggac gacaaccagt tcactcgtga
     4861 cgctggcatc aacactgata acatcgtagc tctgggcctg gtttaccagt tctaatctcg
     4921 attgatctcg aacaaagggc ctgcgggccc ttttttcatt gttttcagcg tacaaacaca
     4981 gttttttggt gtactcttgc gaccgttcgc atgaggataa tcacgtatgg aaataagctt
     5041 tacccgcgtg gcactgctgg ctgccgcgct cttctttgtt ggttgcgatc aaaaaccaca
     5101 acccgccaaa acccacgcta ctgaagttac cgttcttgaa ggcaaaacca tgggtacctt
     5161 ctggcgtgcc agcatcccgg gcattgacgc caaacgcagt gccgaactta aagaaaagat
     5221 tcagacccag ctggatgccg acgatcagct gctttcgacc tataaaaaag attccgcgct
     5281 gatgcgcttt aacgactcgc aaagtttgtc gccgtggccg gtaagtgaag cgatggccga
     5341 tatcgtcacc acctcgctgc gcattggcgc aaggaccgat ggtgcgatgg atataaccgt
     5401 cgggccgctg gtgaatctgt ggggctttgg cccggaacaa cagccggttc aaattccgag
     5461 ccaggaacag atcgatgcga tgaaagccaa aaccggctta cagcacctga cggtcatcaa
     5521 tcagtcgcat cagcaatatc tgcaaaaaga cctgccggat ttatatgtcg atctctccac
     5581 cgtcggtgaa ggttatgcgg cggatcacct ggcacgcttg atggagcagg aagggatttc
     5641 ccgctatctg gtgtcggtgg gcggcgcgct gaacagccgt ggtatgaacg gtgaaggcaa
     5701 gccgtggcgg gtagcgattc aaaaaccaac cgataaagaa aacgcggttc aggctgtggt
     5761 ggatatcaac ggtcatggga ttagcacctc tggcagctac cgtaactatt acgaactgga
     5821 cggcaaacgt ctttcccatg ttatcgatcc gcaaatcggg cgtcccatcg aacacaatct
     5881 ggtatccgtg acggtgattg ctccgacggc gctggaagcc gatgcctggg atactggctt
     5941 gatggtactc gggccgaaga aagccaaaga ggttgttcgc cgggaagggc tggcggttta
     6001 tatgatcacc aaagaaggcg atagctttaa aacctggatg tcaccacagt ttaaaagctt
     6061 ccttgtcagc gaaaaaaatt aaagcgcaag attgttggtt tttgcgtgat ggtgaccggg
     6121 cagactaaag gctatcctta accagggagc tgattatgaa aaaagccaca tgcttaactg
     6181 acgatcaacg ctggcaatct gtcttagccc gcgacccgaa tgccgacggc gaattcgttt
     6241 tcgccgtgcg taccacaggc atcttttgcc gtccgtcttg ccgcgccaga catgctttgc
     6301 gggaaaacgt ctccttctac gcaaatgcca gcgaggcact cgccgctggc tttcacccct
     6361 gcaaacgttg tcagccagaa aaagccaatg cccagcaaca tcggttggat aaaatcaccc
     6421 acgcgtgtcg actgctggaa caggaaacgc ctgtaacgct ggaagcctta gccgaccagg
     6481 tggcgatgag tccatttcat ctacatcggt tgtttaaagc gactaccgga atgacgccta
     6541 aagcctggca acaggcctgg cgcgctcgcc gtttgcgcga atcgctggcg aaaggggaga
     6601 gcgtgacgac gtctattctt aacgccggat tccccgacag cagcagttac tatcgcaaag
     6661 ctgacgaaac gctgggcatg acggctaaac aattccgtca cggtggcgaa aatctggcgg
     6721 tgcgttacgc gctggctgat tgtgagctgg gtcgttgcct ggtggcagaa agcgagcggg
     6781 ggatttgcgc gatattgctg ggcgatgatg acgcgacact aatcagcgag ttgcagcaga
     6841 tgtttcccgc tgccgacaac gcgcctgccg atctgatgtt tcagcaacat gtgcgtgaag
     6901 tgatcgccag cctcaatcaa cgcgatacgc cgctgacgtt accgctggac attcgcggca
     6961 ctgcttttca gcaacaagtc tggcaggcac tgcgcacgat accttgcggt gaaaccgtca
     7021 gttatcagca actggctaac gccatcggca aaccgaaagc ggtacgggcc gttgccagcg
     7081 cctgtgccgc caacaagctg gctatcataa taccctgtca tcgggtggtc cgtggtgatg
     7141 gcacactttc cggttaccgc tggggcgtgt cgcgtaaagc gcaactgctg cgccgcgaag
     7201 ctgaaaatga ggagaggtaa tgttggatct gtttgccgat gctgaaccgt ggcaagagcc
     7261 actggcggct ggagcggtaa ttttgcaccg ttttgctttt aacgctgcgg agcaactgat
     7321 ccgcgatatt aataacgttg ccagccagtc gccgtttcgc cagatggtca cccccggggg
     7381 atataccatg tcggtggcga tgaccaactg tgggcatctg ggctggacga gccatcggca
     7441 aggttatctc tattcgccca ttgatccgca aacaaataaa ccgtggcccg ccatgccaca
     7501 gagttttcat aatttatgtc aacgtgcggc tacggcggcg ggctatccag atttccagcc
     7561 agatgcttgt cttatcaacc gctacgctcc tggcgcgaaa ctgtcgctgc atcaggataa
     7621 agacgaaccg gatctgcgcg cgccaattgt ttctgtttct ctgggcttac ccgcgatttt
     7681 tcaatttggt ggcctgaaac gtaatgatcc gctcaaacgt ttgttgttgg aacatggcga
     7741 tgtggtggta tggggcggtg aattgcggct gttttatcac ggtattcaac cgttgaaagc
     7801 ggggtttcat ccactcacca ccgactgccg ctacaacctg acattccgtc aggcaggtaa
     7861 aaaagaataa aaataagaat tattattgct gtgcgcgcga agatgtttaa actgcgggct
     7921 gtaattcatt gtccgggttt tctgcatgga acttcttgta cttgtctggc ggcagtatcg
     7981 ctggccattt atcagtgtga tggcgctaag cctcgccagt gcggcattag gcattggctt
     8041 aattgctttt atcaatcagc gccttatcga aacggcggat accagtctgc tggtgttgcc
     8101 ggagtttctt ggattattac tgctgttgat ggcagtcact ctcggatcgc aactggcgct
     8161 caccactttg gggcatcact tcgtttaccg actgcgtagt gaatttatca agcggattct
     8221 ggatacccac gtcgagcgca ttgaacaact cggtagcgcc tcgttgctgg cgggattaac
     8281 cagcgatgtg cgcaatatca ccattgcttt tgtgcgtctt ccggaactgg tgcaggggat
     8341 cattctcact atcggttccg cggcgtatct gtggatgctg tcgggcaaaa tgttgctggt
     8401 aacggctatc tggatggcga tcaccatctg gggtggtttt gtactggtgg cgcgggtgta
     8461 caaacatatg gcgaccctgc gtgaaaccga agacaagctg tacacggatt ttcaaaccgt
     8521 actcgaaggg cgcaaagagc tgactctgaa ccgggaacgc gccgagtatg tgtttaacaa
     8581 cctctacatc cccgatgcgc aagagtatcg ccatcatatt atccgcgcag acaccttcca
     8641 tcttagcgcc gtgaactggt caaacatcat gatgctgggc gctatcggcc tggtgttctg
     8701 gatggcgaac agtctcggtt gggctgatac caacgttgct gcgacctatt cgttgacgct
     8761 gttattcctg cgtacgccgc tgctttcggc ggttggcgca ttgccgacgc tgctgacggc
     8821 gcaggtggcg tttaacaagc tgaacaaatt cgcgctcgcg cctttcaaag ctgagtttcc
     8881 gcgcccgcag gcttttccca actggcaaac gctggagctg cgtaacgtga cgtttagcta
     8941 tcaggataac gcgttttccg ttgggccgat taacctcacc attaaacgtg gcgagctgct
     9001 gtttctgatt ggtggcaacg gtagtggaaa atcgacgctg gcgatgttgc tgacgggctt
     9061 gtaccagcca caaagcggcg aaatcttgct ggatggcaaa cctgtcagtg gcgaacaacc
     9121 ggaagattat cgcaaactgt tttcggcagt gtttaccgat gtctggctgt ttgatcaact
     9181 gctggggccg gagggtaaac ccgctaaccc gcaactggtt gagaagtggc tggcgcagct
     9241 gaaaatggct cataagcttg agttaagcaa cgggcgtatt gttaacctga agttatcaaa
     9301 agggcagaaa aaacgcgtgg cgctgttgct ggcgctggca gaagaacgcg atattatcct
     9361 gctggatgaa tgggcggcgg atcaggatcc acacttccgt cgtgagtttt atcaggtgtt
     9421 gctgccgctg atgcaggaga tgggtaaaac tattttcgct atcagtcatg atgatcatta
     9481 ctttatccac gccgaccgcc tgctggaaat gcgcaatggg caacttagcg agctgacggg
     9541 cgaagagcgc gatgccgctt cgcgtgatgc cgttgcccgg acggcataat attacgacgc
     9601 tgacctggcg gactgctgcc gtcaggtcaa tatgctaaaa atcccttcat ttctaataac
     9661 cctataatta atcaacgaaa ttat
//
LOCUS       DAZGAN020000158         9616 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGAN020000158 DAZGAN020000000
VERSION     DAZGAN020000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.158"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..146
                     /gene="ykfM"
                     /locus_tag="ACHKYO_RS22525"
                     /old_locus_tag="pgaptmp_004505"
     CDS             <1..146
                     /gene="ykfM"
                     /locus_tag="ACHKYO_RS22525"
                     /old_locus_tag="pgaptmp_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HGU4431225.1"
                     /translation="PFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDS
                     AVK"
     gene            complement(283..1068)
                     /gene="yafT"
                     /locus_tag="ACHKYO_RS22530"
                     /old_locus_tag="pgaptmp_004506"
     CDS             complement(283..1068)
                     /gene="yafT"
                     /locus_tag="ACHKYO_RS22530"
                     /old_locus_tag="pgaptmp_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4431226.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(1395..1471)
                     /locus_tag="ACHKYO_RS22535"
                     /old_locus_tag="pgaptmp_004507"
     tRNA            complement(1395..1471)
                     /locus_tag="ACHKYO_RS22535"
                     /old_locus_tag="pgaptmp_004507"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1435..1437),aa:Asp,seq:gtc)
     gene            complement(1604..2335)
                     /gene="dnaQ"
                     /locus_tag="ACHKYO_RS22540"
                     /old_locus_tag="pgaptmp_004508"
     CDS             complement(1604..2335)
                     /gene="dnaQ"
                     /locus_tag="ACHKYO_RS22540"
                     /old_locus_tag="pgaptmp_004508"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HGU4431227.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            2400..2867
                     /gene="rnhA"
                     /locus_tag="ACHKYO_RS22545"
                     /old_locus_tag="pgaptmp_004509"
     CDS             2400..2867
                     /gene="rnhA"
                     /locus_tag="ACHKYO_RS22545"
                     /old_locus_tag="pgaptmp_004509"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HGU4431228.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHSWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(2864..3586)
                     /gene="yafS"
                     /locus_tag="ACHKYO_RS22550"
                     /old_locus_tag="pgaptmp_004510"
     CDS             complement(2864..3586)
                     /gene="yafS"
                     /locus_tag="ACHKYO_RS22550"
                     /old_locus_tag="pgaptmp_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4431229.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            3620..4375
                     /gene="gloB"
                     /locus_tag="ACHKYO_RS22555"
                     /old_locus_tag="pgaptmp_004511"
     CDS             3620..4375
                     /gene="gloB"
                     /locus_tag="ACHKYO_RS22555"
                     /old_locus_tag="pgaptmp_004511"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HGU4431230.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            4447..5805
                     /gene="mltD"
                     /locus_tag="ACHKYO_RS22560"
                     /old_locus_tag="pgaptmp_004512"
     CDS             4447..5805
                     /gene="mltD"
                     /locus_tag="ACHKYO_RS22560"
                     /old_locus_tag="pgaptmp_004512"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HGU4431231.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(5853..6623)
                     /locus_tag="ACHKYO_RS22565"
                     /old_locus_tag="pgaptmp_004513"
     CDS             complement(5853..6623)
                     /locus_tag="ACHKYO_RS22565"
                     /old_locus_tag="pgaptmp_004513"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4431232.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(6701..7501)
                     /gene="yafD"
                     /locus_tag="ACHKYO_RS22570"
                     /old_locus_tag="pgaptmp_004514"
     CDS             complement(6701..7501)
                     /gene="yafD"
                     /locus_tag="ACHKYO_RS22570"
                     /old_locus_tag="pgaptmp_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU4431233.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            7742..8656
                     /gene="yafC"
                     /locus_tag="ACHKYO_RS22575"
                     /old_locus_tag="pgaptmp_004515"
     CDS             7742..8656
                     /gene="yafC"
                     /locus_tag="ACHKYO_RS22575"
                     /old_locus_tag="pgaptmp_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HGU4431234.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVAMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(8653..9456)
                     /gene="dkgB"
                     /locus_tag="ACHKYO_RS22580"
                     /old_locus_tag="pgaptmp_004516"
     CDS             complement(8653..9456)
                     /gene="dkgB"
                     /locus_tag="ACHKYO_RS22580"
                     /old_locus_tag="pgaptmp_004516"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HGU4431235.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAKDKKAIAALDCNDRLVSPKGLAPEWD"
BASE COUNT         2591 a         2254 c         2259 g         2512 t
ORIGIN      
        1 ctccttttgt cacaggatat gtcaatgcta attatcaccc ctgcggtaat aatacgggga
       61 ttttcccagg agcaatctat attaaaaatg gcatgaaatg taataatgaa tatatttctc
      121 gcaaggaaga ttcagcagtt aaataattaa atattctaaa acaaacagtt tttaactccg
      181 gattccatgt cctttcttgc actgtctttg tcaagaaaaa tttcttatgc aaagaatttt
      241 gtattattgc cgccagaaat tcctggcggc agatataatt tattattgat agcggttaac
      301 caaatctttt accatcgctt tataggtctt ttgcttaagt tgcatgattt gctgctctgt
      361 catatcagtc gtactggctt cattcttgtc gggcaatggt ggaagcactt tatattcata
      421 attcaccggc gaccaggtag cccactcacc tgttgccaca tccaccagcg tgaaacgtac
      481 caggtagcgc agggagatag cgtcggtgag tttttcattt ttatagtcgg accatactgt
      541 tgatttcagc gcagagtcat attttcctgt ctgcaatgta tcctggtaaa caataatcgc
      601 tttctgatgt ccttttgcag ccacaaaacg cagtgcctgc atccagttca tattctcagc
      661 actggcaaca tcctcgttct catttttatc tttgtttttg ttacaagtca gaggcttttg
      721 cctgtccggg ataccagaga atgtggaaac agtataatat tttcgcatct cctcctgcat
      781 aatggtttcc ggtgcgcggt tgccggattg aacaagtatt acaggagaat taaggggaac
      841 gcggaactca tctccatcaa gcgcagcttg aatatcctct tcggatacgg ccagagtggt
      901 ttcattaccg aataaatctt tgtctgtcag ttgctgggga gtgtttttat taatattact
      961 ttgtgtgact tgagcttttt tcttcttttc atcaatagaa gattcagagg cacatcctgc
     1021 aagcagcgag aataacacac atatgcaaca aagctttttt gaattcataa ttggacactc
     1081 cctcgcctgt cataatgatt agaatattta gcattgataa cggactctaa ttatataccc
     1141 cacttaaata ataacaaaca aaatcaattt gaaatataca ttcatttaat caatataaat
     1201 ttattctctg gaaatatatt taagctgaat ggttcgatat cattattgaa ttttaatgaa
     1261 tgagacaaca agcgagaatc acgcacttac gccagaataa aatcattgaa cagagaaatg
     1321 gtggaattca aatacaaaaa aaccgcaaaa ttaaaaatct tgcggctctc tgaactcatt
     1381 ttcatgagtg aatttggcgg aacggacggg actcgaaccc gcgaccccct gcgtgacagg
     1441 caggtattct aaccgactga actaccgctc cgcgttgtgt tccgttggga acgaggcgaa
     1501 tattacggat tgcctcaccc ttcgtcaacg gtttttctca tcttttgaat cgtttgctgc
     1561 aaaaatcgcc caagccgcta tttttagcgc cttttacagg tatttatgcc cgccagaggc
     1621 agcttccgcc cttcttctcc accagatcaa gacgggcttc atgagctgca agctcttcat
     1681 ctgtcgcaaa aacaacgcgt aacttacttg cctgacgtac aatgcgctga attgttgctt
     1741 caccttgttg ctgctgtgtc tctccttcca tcgcaaaagc catcgacgtt tgaccaccgg
     1801 tcatcgccag ataaacttcc gcaaggatct gtgcatcgag taatgccccg tgtagcgttc
     1861 gtttactgtt atctatttcg tagcgggcac ataacgcatc gaggctgttg cgcttaccag
     1921 gaaacatttt cctcgccacc gcaaggctat cggtgacctt acagaaggta ttggtcttcg
     1981 gaatatcgcg cttaagcaac gaaaactcgt agtccataaa gccgatatcg aacgctgcgt
     2041 tatggatcac caactccgcg ccgcggatat agtccatgaa ctcatcggct acttcggcaa
     2101 acgtgggctt atcgagcaaa aattcatcgg caataccatg tacgccaaag gcttccggat
     2161 ccaccagccg atcgggtttg agataaacat ggaagttatt gcccgtcagg cgacggttca
     2221 ccacttcaac ggcaccaatc tcaatgatct tgtggccttc atagtgcgca ccaatctggt
     2281 tcataccggt ggtttcggta tcgagaacga tctggcgtgt aattgcagtg ctcatagcgg
     2341 tcatttatgt cagacttatc gttttacagt tcgattcaat tacaggaagt ctaccagaga
     2401 tgcttaaaca ggtagaaatt ttcaccgatg gttcgtgtct gggcaatcca ggacctgggg
     2461 gttacggcgc tattttacgc tatcgcggac gcgagaaaac ctttagcgca ggctacaccc
     2521 gcaccaccaa caaccgtatg gagttgatgg ccgctattgt cgcgctggag gcgttaaaag
     2581 aacattgcga agtcattttg agtaccgaca gccagtatgt ccgccagggg atcactcagt
     2641 ggatccatag ctggaaaaaa cgtggctgga aaaccgcaga caaaaaacca gtaaaaaatg
     2701 tcgatctctg gcaacgtctt gatgctgcat tggggcagca tcaaatcaaa tgggaatggg
     2761 ttaaaggcca tgccggacac ccggaaaacg aacgctgtga tgaactggcc cgtgctgcgg
     2821 caatgaatcc cacactggaa gatacaggct atcaagtcga agtttaagcc tgtggtttac
     2881 gatattgccg ggtggctcca accgcctggc gaattcgtgg cttgttttta ctctgtttca
     2941 tcggatttag cgttaaagga atagtccgtt tccgggcaac aataagttgt aagcaaccaa
     3001 gcgcaggaat atgtgcattc aatagttttc caccgtgttt gttccacggg agaacgtgga
     3061 aacggctggc gtgcagcact tcaaaattca acaaagagag ccagtccagt tgccgcatca
     3121 gagtaaacat ccggctgtta tagggcgagg ttttgcgcaa taccggcaca agtttgcgta
     3181 atcccattaa actgatggga ttgaagccac taatcaccag ccagccatca tcaatcaata
     3241 cccgatcggc ttcacgcaat aaacgatgcg gatcggtgca ccacggcaat gtatgtgcca
     3301 gtagacaaac atcaacggat ttatcggcaa aaggaagatg aagtgggtcc gcctgtacct
     3361 ggacgggcat tccttgcgca gaaacattca cttgatgaga aaccgcgcac gcttcgcaat
     3421 tgatttctgc gcttaaattg ccaatcttaa gcagatgaaa accatacatt ttagtgaacc
     3481 acgggttgag ctggcgctcc agcgccttgc gataaagctc tccccagggc aaatcgcccc
     3541 agcaatcagg agccacgaca gtttgaggga ctcttgccgg tttcatcaca accttccgtt
     3601 tcacactaag aggtaatcta tgaatcttaa cagtattccc gcctttgatg acaattacat
     3661 ctgggttttg aatgatgaag caggtcgctg cctgattgtc gatcccggag acgcagagcc
     3721 agtattaaac gccattaccg ccaataactg gcaaccggag gccatatttc tcacccacca
     3781 tcatcacgat cacgttggcg gcgtaaaaga actggtggaa aagtttccgc aaattgtggt
     3841 gtatggtcca caagagacac aagataaggg aacaacacag gtagtcaaag atggcgaaac
     3901 tgccttcgtt ttggggcatg aatttagtgt aattgccaca ccgggtcaca ctttaggaca
     3961 tatctgttac ttcagtaaac cttatctatt ttgcggcgac acgctgtttt ctggtgggtg
     4021 tggtaggttg tttgaaggga cagcatcaca aatgtatcaa tcacttaata agttaagtgc
     4081 gttacctgac gatacattgg tatgttgtgc tcatgaatat acgttatcaa atatgaagtt
     4141 tgctttgagt attcttccgc acgatttgtc cataaatgat tattatcgta aagttaagga
     4201 gttacgggca aaaaatcaaa taacactacc cgtaattctg aaaaatgagc ggcaaattaa
     4261 tgttttttta agaacagaag atattgattt aattaatgta attaatgaag aaacattatt
     4321 gcaacaacct gaagaacgtt ttgcatggtt aaggtcaaag aaagataggt tctgataaaa
     4381 ctttcttgtc atcggctccg ttcgccgtta tgatcggtcg tcttttaagc aactattgac
     4441 acacacatga aggcaaaagc gatattactc gcctctgtcc tgctcgtggg ttgccagagt
     4501 accggcaacg ttcaacagca cgcacagagc ctttctgcag ctggtcaagg ggaagcagca
     4561 aagtttacaa gtcaggcacg atggatggac gatgggacgt ctatcgcgcc agatggtgac
     4621 ttgtgggctt tcattggcga cgagctaaag atgggaattc cggaaaatga ccggattcgc
     4681 gaacagaaac agaaatattt acgcaataag agctatctcc acgatgtaac tttacgggca
     4741 gagccgtata tgtactggat agccgggcaa gttaaaaaac gtaacatgcc tatggaactg
     4801 gtactactac ccatagtgga gagcgctttt gatcctcacg caacgtctgg cgccaatgcc
     4861 gcaggcatct ggcagatcat tccgagcacg gggcgcaatt atggtttgaa acagacccgc
     4921 aattatgacg cgcgtcgcga tgttgttgct tcaacaactg ccgcgctgaa catgatgcag
     4981 cgtctgaaca agatgtttga cggcgactgg cttctgaccg tagcggctta taacagcggc
     5041 gaaggtcggg tcatgaaggc aattaaaacg aacaaagcgc gtgggaaatc cacggacttc
     5101 tggtcgttac cgttgccgca ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt
     5161 gatattctca aaaacagcaa gcgttatggc gtacgtctgc caacgaccga tgaaagccgt
     5221 gctctggcgc gtgtgcacct gagcagcccg gttgaaatgg cgaaggttgc agatatggcg
     5281 gggatttccg tcagcaagct gaagacattc aacgctggcg tgaaaggctc cacgctgggc
     5341 gcaagtggtc cgcaatacgt gatggtgcca aagaagcatg cagatcaact gcgtgaatct
     5401 ctcgcttcag gcgaaattgc tgctgtacag tcgacgctgg ttgccgacaa tacgccgctt
     5461 aacagccgtg tttacaccgt acgctctggc gacacgcttt caagtatcgc ttcacgtctc
     5521 ggcgtaagca ccaaagattt gcagcagtgg aacaaactgc gcggatctaa gctgaagcca
     5581 ggccaaagtt tgacgattgg tgcaggcagt agcgcacagc gactggcaaa caacagcgat
     5641 agcattacgt atcgtgtgcg caaaggcgat tcgctttcaa gcattgctaa acgccacggc
     5701 gtgaacatca aagatgtgat gcgctggaac agcgacactg cgaatctgca accaggcgac
     5761 aagctgacgt tgtttgtgaa aaacaacaac atgccagatt cctgacaaac cagataataa
     5821 aaaggcaccg attcccccgg tgcctttttt atttatgctg ctttatgtgc ttctaccatg
     5881 atgatatcac tggtgaaaga gccatcattc tgcaaggcaa aatacgttct cacctctgtc
     5941 gatgcgctct gttggtaaat gcgaatagcg tctactaacg cttctggcgt acgcattctc
     6001 gcgacccatg aagaaaattc cagcggtaac ttatctgtaa ttaaattatc aactatcaga
     6061 ttggcttcat tgattaacct caaccactca ccgctggcgt agtttcgtac gtgagaggta
     6121 tcgcgtaatg cttctaccgt ctgtaaccag atgtcgcgca ctggatgacc cggagacatt
     6181 acgtccatca caatcatcct accgccaggt ttcaatatcc tattcacttc tcgcagtgct
     6241 gcaccaacat catgccagtg atgggcagaa taacggctga taacaatatc aaatgcgtta
     6301 tcggcaaatg gcagactttc ggcataaccc tggcgggtgg tgatattttt cagttgccgg
     6361 gcttcggcag cttgtgccac gacatccagc atttgggcag ataagtcata cgccaccacc
     6421 gcgctcacgt tctgcgcagc gacaaagctg gcatgccctg ctccgcagcc catatcaagt
     6481 acacttgcat caggataatc agccagacgc accgccaggc gctgtaaatc tcgcccggat
     6541 gcatgtacgg tactggttaa atactcactg gcctgcgagc taaactgctt ttctacgtgg
     6601 tcatggtggg attgtgttgt cattgcgctg tccttttgtt gtttgagtat aaaaggacag
     6661 cgccaaacgt tggtctgccc tgtggcagac ctgacatacc ttatttatca ggcttgccgg
     6721 gactgaattc aacgagtagc ggattgtgat cggaagcgcg cgtaaccaat acagaagctt
     6781 cgctgacgtt cagaccacgg tagaaaacaa aatcgagcgg gcggccaaac gcccgacggc
     6841 gctgatcatc ggtaaaacgc acctggcgca gcgacatttc ccgcgcaaag cgatataacg
     6901 cgttcatcct tctacggctc caggcattga aatctcctgc cataatgacc gggccgctgt
     6961 ggtgagctat ctgatcacca ataggaagta actgcttact atagacatcc acgcccagac
     7021 tgaagttgac ggcgtgtata ttaaccacca tcaacaggcg ggtgtcaggt aatggataga
     7081 ccgtcaccag tgccgacttc gccagacgca aaatgggttc tcgttcacgt aacgggcagc
     7141 aatacactgg atgcgccgcc gaaagggtca ttacgccaga aggatgttgt ggcagtacga
     7201 aagcgggtac ctgatcggcg gcaagatagt tagcggtcgc aaactgtact aactctggcg
     7261 ttgtctgcgc ttcctgcaat aacaccagat gtgcatcttt gccgtagttc tttaataccg
     7321 acaaccattc agcgcgttgc tgtttgtata tgttccacac caggatccga atacgctctt
     7381 cggtacttaa cggttctccg ggtggtaatg cctggccgat gctcgcaaaa gaccccggcg
     7441 gtaagatcct ttccgcaggt tgtccggcaa catagcgcat ggcataggtg ttttttcgca
     7501 cttttgactt tgaaacctct attacgtcaa cccttccgca ctcgggaggg ttctactgtt
     7561 atagggattt tagctcactc tttcaacgag caattgtata ttgttatgta agcaagtgct
     7621 tacaccggtc agaattttgc tcattatgga gaacattaaa tcgcatgaca tatcacgcat
     7681 cctgatggtt taatgacaaa taaattcaaa atatctcttc tattttgcaa caggagcaaa
     7741 aatgaaagcc acgtcggaag aactcgccat ttttgtttcg gtcgtcgaaa gcggcagctt
     7801 tagccgggca gcggaacaat tagggcaagc aaactcagcg gtaagccggg cggtgaaaaa
     7861 gctggagatg aaacttggcg ttagcctgct taatcggacc acgcgacaac ttagcctgac
     7921 ggaagaaggc gagcgttatt ttcgtcgcgt acagtcaatt ttgcaggaga tggcatcggc
     7981 agaatcagaa attatggaga cgcgtaatac accgcgtgga ctgttacgga tcgatgccgc
     8041 aactccagtg gtgctgcact ttctgatgcc gttaattaag cctttccgtg aacgctatcc
     8101 ggaagtcact ttgtcgctag tctcctccga aacgattatt aatttgatcg aaagaaaagt
     8161 ggatgtcgcg atacgcgctg gtacgttaac ggattccagc ttacgtgcca ggccgttatt
     8221 taacagttat cgaaaaatta tcgcctcccc cgattatatt tcccgctacg ggaagccaga
     8281 aacgatcgac gatttaaagc aacatgtttg cctgggattc actgaacccg cttccctcaa
     8341 tacctggccg atagcctgta gtgatggaca attacatgag gtgaagtacg gtttgtcatc
     8401 caatagtggg gaaacactga aacagctttg cctgagtggc aacgggattg cgtgtttgtc
     8461 cgactacatg atcgacaaag aaatcgctcg cggagaattg gtggagttaa tggcagataa
     8521 agtgttgcca gtggcaatgc cattcagtgc agtctattac agcgaccgtg cggtaagtac
     8581 gcgcatccgg gcttttatcg atttccttag cgagcatgta aaaacagctc ccggaggagc
     8641 tgtcagagag gcttaatccc attcaggagc cagacctttc gggctaacca ggcggtcgtt
     8701 gcaatccagg gcggcgatcg cttttttatc tttggcatcg agctgtaaat tttgtgcctt
     8761 aagattactt tccaggtttt tacgtttagt agaagaagga attactgagt aaccttcccc
     8821 catagcccac gccagaatca cttgtgccgg agtcgcattg tgtttagctg cgatacgagc
     8881 aataacctca tctttcaggg ccttaccata cgccagcgtc atataggaag taatatggat
     8941 gccgtgctgt ttagcccagg caaccacttt acggttttgc agataaggag agagttcaat
     9001 ctggttagta gcgatgtttt cagcgccaac agcagcaatc gccttttcca tcaatgggat
     9061 cgtgaagttg gaaataccga tctcacgcgt cagcccttgt tttttggctt ccagcagcgc
     9121 ctgcataaac tcttcaacag agacttcatc gtttggtgac ggccagtgga ttagcgtcag
     9181 atcaacataa tcggtacgca atttttgcag gctctctttc agacttggga tcaatttgtc
     9241 tttgctgaga ttttcaatcc agattttagt ggtgatgtag agttcatgac gtggcacgcc
     9301 actttctgca atcgcctgac ctactgcggc ttcgttatca tagatttgtg cggtatcaat
     9361 tgcgcgataa ccaagttcaa gcgccgtttt cacagatgaa ataacaacgt cgtctttcag
     9421 acggaaagta cctaaaccaa atgcagggat agccataata tgcctctttt aatgctattt
     9481 tcgttaactg aggtgattat gggcttcaga ctgttgagga aaaagagcgt aaaatgcaga
     9541 ggatttttgc gattctggca ataatagata tacagacgaa aaaaaacctc gagcgttaac
     9601 tcgaggcttc ttaata
//
LOCUS       DAZGAN020000159         9318 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGAN020000159 DAZGAN020000000
VERSION     DAZGAN020000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.159"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..143)
                     /locus_tag="ACHKYO_RS22585"
                     /old_locus_tag="pgaptmp_004517"
     CDS             complement(<1..143)
                     /locus_tag="ACHKYO_RS22585"
                     /old_locus_tag="pgaptmp_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431236.1"
                     /translation="MLQSSADEACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFS"
     gene            221..1315
                     /gene="ddlA"
                     /locus_tag="ACHKYO_RS22590"
                     /old_locus_tag="pgaptmp_004518"
     CDS             221..1315
                     /gene="ddlA"
                     /locus_tag="ACHKYO_RS22590"
                     /old_locus_tag="pgaptmp_004518"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU4431237.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLLTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1339..1551)
                     /gene="yaiZ"
                     /locus_tag="ACHKYO_RS22595"
                     /old_locus_tag="pgaptmp_004519"
     CDS             complement(1339..1551)
                     /gene="yaiZ"
                     /locus_tag="ACHKYO_RS22595"
                     /old_locus_tag="pgaptmp_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HGU4431238.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1811..2119
                     /gene="yaiY"
                     /locus_tag="ACHKYO_RS22600"
                     /old_locus_tag="pgaptmp_004520"
     CDS             1811..2119
                     /gene="yaiY"
                     /locus_tag="ACHKYO_RS22600"
                     /old_locus_tag="pgaptmp_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HGU4431239.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2178..3272)
                     /gene="yaiW"
                     /locus_tag="ACHKYO_RS22605"
                     /old_locus_tag="pgaptmp_004521"
     CDS             complement(2178..3272)
                     /gene="yaiW"
                     /locus_tag="ACHKYO_RS22605"
                     /old_locus_tag="pgaptmp_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HGU4431240.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3285..4505)
                     /gene="sbmA"
                     /locus_tag="ACHKYO_RS22610"
                     /old_locus_tag="pgaptmp_004522"
     CDS             complement(3285..4505)
                     /gene="sbmA"
                     /locus_tag="ACHKYO_RS22610"
                     /old_locus_tag="pgaptmp_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HGU4431241.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4857..6014
                     /gene="ampH"
                     /locus_tag="ACHKYO_RS22615"
                     /old_locus_tag="pgaptmp_004523"
     CDS             4857..6014
                     /gene="ampH"
                     /locus_tag="ACHKYO_RS22615"
                     /old_locus_tag="pgaptmp_004523"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HGU4431242.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6015..6638)
                     /gene="iprA"
                     /locus_tag="ACHKYO_RS22620"
                     /old_locus_tag="pgaptmp_004524"
     CDS             complement(6015..6638)
                     /gene="iprA"
                     /locus_tag="ACHKYO_RS22620"
                     /old_locus_tag="pgaptmp_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HGU4431243.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6726..>9318)
                     /locus_tag="ACHKYO_RS22625"
                     /old_locus_tag="pgaptmp_004525"
     CDS             complement(6726..>9318)
                     /locus_tag="ACHKYO_RS22625"
                     /old_locus_tag="pgaptmp_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4431244.1"
                     /translation="DYYHDRLALTVDNSTIDDNYEHYTYNGTYNNAADTHVVDVYNIG
                     TAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSSEVFNNTITVKDST
                     VTSGSWSDEGTSGWFGNTGNASDYNGGDWNRDDIALAVIAHPAADNAMQTTATFDNST
                     LMGDVFFSSNFDENFFPHGRDSYRDADGDVDTNGWDGTDRLDLTLNNGSKWVGAAMSA
                     HLTVDTNDDGVPDAYGPVYNDNGVVIDTSTGTEATATLIDIAANSLWPSSTVGVENSD
                     SEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADSTLIS
                     DSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKVTND
                     GILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTNDTTK
                     SNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRIDNA
                     TGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDY
                     ANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYD
                     QDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFAN
                     NVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYG
                     SISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVY
                     DDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSAN
                     VGVKYTW"
BASE COUNT         2369 a         2338 c         2245 g         2366 t
ORIGIN      
        1 gaaaaaaact caccgcatgg ccatttaagc caggaataaa ttagcttccc gttcatcatt
       61 tttgtgatct tgcgcacatc tttgagataa cgcactttct tcaccagtac gttgcacgct
      121 tcgtcagcag aagattgcaa caatctttca gaaggccgcg aatcccccgt tttgtcgtgg
      181 taatgtatgc actttgtgtt agacgggata ggtttttaag atggaaaaac tgcgggtagg
      241 aatcgttttt ggtggtaaat cagcggaaca tgaagtgtct ctgcaatcgg caaaaaacat
      301 tgtcgatgcc attgataaaa gtcgcttcga cgttgtgctg ctgggcattg ataaacaagg
      361 gcaatggcac gtcagcgatg ccagcaatta tctgctaaat gcagacgatc ctgcccatat
      421 tgcgttacgc ccttcggcga ccagccttgc gcaggtgcca ggtaaacatg agcatcagct
      481 tatcgacgcg caaaacggtc agccgttgct gacggtggat gtcattttcc cgattgtcca
      541 cggtacgctg ggcgaagatg gttccttgca gggaatgctg cgggtcgcca atttaccgtt
      601 tgtaggttct gatgttctgg cttcagcagc ctgtatggat aaagatgtca ccaaacgtct
      661 gctacgcgat gccgggctga acattgcgcc atttattact ctgacgcgcg ctaatcgtca
      721 caacatcagt tttgccgaag tggagtctaa actggggtta ccactgtttg taaaaccggc
      781 taatcagggc tcttctgttg gtgtcagcaa agtaaccagt gaagaacagt acgcaattgc
      841 cgtcgatctg gcgttcgagt tcgatcataa agtgatcgtt gagcaaggga tcaaaggtcg
      901 tgagatcgaa tgcgcagttc tgggcaacga caatccgcaa gccagcacct gtggcgagat
      961 cgtactcacc agcgatttct atgcctacga caccaagtac attgacgaag atggcgcgaa
     1021 agtggtagtt ccggcagcca ttgcaccaga gatcaacgat aagatccggg cgattgccgt
     1081 tcaggcttat cagacgttgg gatgcgcagg catggcgcgt gtagacgtgt ttttaacccc
     1141 agagaacgaa gtggtgatca acgagatcaa cacactgcct ggcttcacca acatcagtat
     1201 gtatccgaag ctgtggcaag ccagcggtct gggttacacc gatctgatca cacgtttgat
     1261 tgagctggcg ctggagcgtc acgctgcgga taacgcattg aaaaccacaa tgtaatattt
     1321 taacgcccga taatattctt attgggcgtt ttcagtttct tcatcgcgac gacgaataat
     1381 caaccccgcc agccagaaac taatcaccca cgtcaccaga ccgacggcat aggtctgcca
     1441 accttttgct tcaaatccca gtagccccac cacgccatta agaatgaata taaggccaat
     1501 ggcgaacgca tagtagtgcc agtcacggcg gatttttaca ggcagattca tggcagctcc
     1561 gttaacgaaa aacaatatcc ttgcatattc tgtaacaacc gtctgtggtg tagacaacat
     1621 ttttgtaatt gttaataaat ttaacttaag gttgccattt tgtgactcac aacgaagatt
     1681 cacgcttcgg gaatattccc aacatcaatt taccatgatt ctgatattga caatctaaat
     1741 gacctgtcag acttgccata agccgctggt atcacaccag aagagaagca gatgacttcc
     1801 ggaggttttt atggctgatt tcaccctgtc aaaatcgctg tttagcggaa aatatcgcaa
     1861 tgcctcttca acgcctggca acattgccta tgcgttgttt gtgctgtttt gcttttgggc
     1921 tggggcgcaa ttgctgaacc tgttagtgca tgcgcccggc gtctatgagc gtttaatgca
     1981 ggtccaggaa acaggtcgcc cacgggtgga aattggttta ggtgtcggca ccattttcgg
     2041 gctgatcccg tttttagtag gctgcctcat ttttgcagtg gtggcgctat ggctgcactg
     2101 gcgacatcgc cgccagtaag atttacgttg ccagatacgg cctaaaacgc cttatctggc
     2161 atgacattcc tgtttgatca ctgtttcata caacgcgccc gccgttcgtc tacgcgcttc
     2221 gcaaaccagg ccgtagtcag gttgcgggtg attttcgggc tttccagttg aatgccaggc
     2281 aacatttcgc ggggtagtga tttacccgtt ttcgcttcgg caagttgata aactttcttg
     2341 tacagcgccg tctcctcaaa agagaagctg tcacctttct ctaactgacg gcgaatttcg
     2401 ctgtcgctca tccccagttt tcccgccagt ttgcgcgttg ccagttcggt tttcccgggt
     2461 tctttactgt cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc gctggcctta
     2521 ctgactgcgt tctgaaacgc ggcattacgg ctggcgtacc agccagcgtt aaaatcagca
     2581 aagcggtata tcggtgcgct atagctggcg ggataattca gtaaatggta agtaccaaac
     2641 cacaacccgc cgcggcgact gaagacttcc tgacgaactg taccgtccat tttccacgga
     2701 taccctttgg tatgctgttc ggcaaaagca atgctgacct gcatcggccc accggtgcgc
     2761 accggattaa gcgaaccaaa cagcgtctgc cccattggta ccatgctgat cagatcgtca
     2821 aaaatcgcgc tcaattgctt ttctgtacgc accgaatcta accgttcgct ataacttttg
     2881 ccgtttggcg atttgatttt tagcgcggta tgaaccagaa aagcaggaat gtgcatccgt
     2941 tcagcacgac ggtcaatctc ttgccaggcg atcttgctta agcccggaac ggccggatct
     3001 gcctgataat ttgactcctg ctgcgccacc gccagcaccg agcagacgtt ttccagcgtc
     3061 ggtgccagcc cctgactttc aaaggtggtg gcgagatctt tcgcccaggc atcgcggtct
     3121 ttcacgctcg caggcatctt ctgccgcacg acgctcgcta catctatcgc tttttcgccc
     3181 tttttcaacg gttgcggagc ctgactacta caccccgcta aaaccagaac cgccaacaat
     3241 gacagcgaag atagatgatt gacgcgcgac attacttctc ctttttagct caaggtatgg
     3301 gttacttcct gaattttgtc accatccagc tcatgttcaa agctgcgcag acgtttgtag
     3361 atagacatca actcaaccag tgtggtccat gagttaatca ggtactggaa agcaccgcga
     3421 acctgaccaa aaacgttggt aatctgcgtc atcaggccga gcgtaatcgt accggcaaca
     3481 attgacggaa acagcaagaa caaaccgaaa acgttatcga cctgcaaata gagaatgcgg
     3541 gcgatgttga aatacatata gtgaaaatag aggcgaaaat agtttttccg tacggcgcta
     3601 aacagctcgc gtaccgtagg cggcgtcgcg cgcgtggcat cgtcttcacc ataaaccagc
     3661 tctttacggt aggcggcctc tactcgctgg tttttaaact ccagccccgg cagtttgatc
     3721 cctaccactg ccagcaatcc ggtccccatc agcgaccaga cgatagcggc aatcaccaga
     3781 ccatacggaa tgtgcccgac aattggcagc tccggcacat gtgcggagag cgttaccagc
     3841 accggcagga aggcgatcaa cgtcatgatg gcgttgataa aactgacgcc catattctcc
     3901 agcgttgaag caaaacgcat ggtgtcttcc tgcacacgct gtgcggcccc ttcgatatga
     3961 cgcagttgtt gccagttcgc catgtaatat tcgttcatcg ccgtacgcca gcggaacacg
     4021 tagtgactga caaagaagtt gttcagtaca ctgatcacca ccgcgatcag cgcaatcccc
     4081 agaaagacgc ccacttcgcg gtaaaattgt tcgatagtga ctttatgcgg cgaacttagc
     4141 gcggtttgaa tcagatcata gaacggtgca taccaggcgt tgacggcgac cccgacttcc
     4201 accaaaaacc aggtgacgaa gatgatcagt gcagtaccga gtattgacca gtattgccaa
     4261 cggtgcgggc tgtagataaa ccagaaaaat gcaaaaagtc ctacgcaaac aatgtagtaa
     4321 gcgtaaaaaa tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg gatctgcccg
     4381 gaagcgccgg tgatacgcgc cacccagtct cccccaccgg cttgccagaa gataacggca
     4441 atcaatgccc aaacaaaggc cgagagaaaa aacgctcccg gctttgggaa aaaagactta
     4501 aacatatgca ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat taaccgcatg
     4561 accactacaa agcgtcagcc aaatgaaacc gcccattgtg acccatagca aggcgcttag
     4621 ttcgccagcc tgaccataag aattgtttcg acggatttca caaaccaaag attgtttaca
     4681 taagcggatt aacacgcgca ggatcacaaa tgcaggcatt ggtgagcagg aaaaagatcg
     4741 tgtttacgcg ccgttacgtt tttaacacta ttattgcact aattcgccct ttgcaatcca
     4801 tcaatgagta gtataaatac gctcagttac cttcattcaa tctatggaca ccaccgttga
     4861 aacgtagtct gcttttttct gccgtgctgt gtgcggcgtc attgacctcc gtccatgcgg
     4921 cgcagccgat cactgaaccg gagtttgcct ctgatattgt cgatcgttat gccgatcata
     4981 ttttttacgg cagcggtgcc acggggatgg cgctggttgt tatcgacggt aaccagcgcg
     5041 tctttcgtag ttatggcgaa acgcgacctg gtaataacgt tcgcccgcag ctggattcgg
     5101 tcgtgcgtat tgcttccctc accaaactaa tgaccagtga aatgctggtg aaattgctcg
     5161 accaggggac cgtaaagtta aacgatccgc taagtaaata tgctccgcca ggcgcacgcg
     5221 tgccgaccta caatgggacg cccattaccc tagtgaatct ggcaacccat accagcgccc
     5281 tgccccgtga acagcccggt ggcgcggcac atcgtccggt gtttgtctgg ccaacgcgcg
     5341 aacaacgctg gaaatacctt tctacggcga agctaaaagc cgcgccaggc agtcaggcgg
     5401 cgtactctaa ccttgcgttc gacctgctgg cagatgcact ggcgaatgcg tccggcaagc
     5461 cttataccca gttgtttgaa gagcagatta cccgtccatt ggggatgaaa gacaccacct
     5521 acaccccctc accggatcag tgccgccgtt taatggttgc tgagcgcggt gccagtccgt
     5581 gcaataacac gctggcggca attggcagcg gcggcgtcta ttccacgcct ggcgatatga
     5641 tgcgctggat gcagcagtat ttgtcatctg atttttatca gcgcagtaac caggcagatc
     5701 gcatgcagac gttgatttat cagcgcgcgc agtttacgaa agtgattggc atggatgtcc
     5761 ccggcaaagc ggatgcgctt ggtttaggct gggtatatat ggccccgaaa gagggtcgtc
     5821 cggggattat tcagaagaca ggaggtggtg gcggtttcat tacctatatg gcgatgatcc
     5881 cgcagaagaa tattggcgcg tttgtggtgg tcactcgttc gccgttgacg cgctttaaaa
     5941 atatgagcga tggcatcaat gatctggtga ccgagctaag cgggaataaa ccgttggtta
     6001 tccccgcatc ctgattaata ctctgaaggc aaacggttga tagcgaccag tttgccttta
     6061 ttcatttcga tatagccgcc tttacgcaaa gcagcgagaa cttctgcgac gacagaacgc
     6121 gatatgcgtg tacgttgatg gatataattc atcacaccaa tacgtgatcg caattcttca
     6181 ttccagtcaa tcattgataa taatgttgcg cggatttgtt cgtaggaatt atgcccaatg
     6241 agctgcacgt cgcgtaattc cagaattcta ttttgccagg ctaaccagta aaaagcgtct
     6301 cgccagagtt gattttgttc aataagcgta atggtttgtt tggctggtag atgatatccc
     6361 gtacaatttc cttctgatat taatttgtat ggtatatcgt ttttcattaa accatcagcc
     6421 agccccataa tataaggagc ctgggtaata ccgataagta cgttttcttc tctacgcaga
     6481 gagataacgc cctctaaaat aacgaaagta tcttcgctat tgacatcact ggaaaatata
     6541 acttgctttt cattattaaa ctcgaagcgc gtaccgtatc tggacaaaca tttatcgagc
     6601 ttaccaaatt cctgaagagg tttaactaca gataacattt gcgcgtcctt tgcagtaatg
     6661 cccgtcaaat ccttgtcggg cattatttag attaaattac cagaatttct tcggagtgaa
     6721 gaatattacc aggtatattt aacacccacg ttcgcggacc agtcttgatc tacgtcacca
     6781 ccaccgaggt agttagcatc ggtataggcg ctgaagttct tggtgaagct gaactgagta
     6841 cccagaccaa cacgtaccgc agacccttca gtaccgttat cgatggaatc accgttcaca
     6901 tcgttatcgt tgttagagtc gtcgtagacg taagccagtt tgaagtacgg agtcagagcc
     6961 tggtcttcgc tgtaggtgaa ggtataacct gcatctacac ccagttcata acgcatgctg
     7021 tcgtaagact gaccgtcaac tttcatgtcg ttgctcagct ggtagtcatc accagactgg
     7081 aacagaccag aaatgctgcc gtaaggagtc acgtaaccag catcacccag tttgaagtcg
     7141 taaccggctt tcaaaccgaa gccccaagcg tcggagttgg tgctaccgtc aacgtaagta
     7201 ccgttgctca tggttgcaga caggtcgttg ttgaagtgag agtaactcaa gctaccatca
     7261 acaaagacgt tgttcgcgaa gtgagcagaa gagtagatgt aggcagtctg gctgtcttga
     7321 tccacctgac cagaacggtc attcatgtca cctttagcga agcctgcagc cgcaccgacg
     7381 atccacttag cgttgttacc gtcaattttg gtatcaacac cgaccatgat gccgttaaca
     7441 tcctgatcat agttgatggt gccgttgtcg ccgttgaagt taccaccgaa gtagcttacc
     7501 catgcgccgc cgttatcagc caggccatga cgagagttgg tcagacgagt accaacggtg
     7561 tcttgttcca ggttccagat attggtgttc gcagacggga tgctcagcgc catgttagcg
     7621 tagtcggtca gctccatctg ttgcagaaca acggtgttac cgcgctgttc agcctgatag
     7681 gtgtatgcac ccaggtcagc tttgttagca gcagagaagg tcgcgttgct gtttttgtcg
     7741 ttgacgtaga tcagctcgtt gccgttgtag tcagcgatag aaccagcacc agtagcgttg
     7801 tcgatacgaa ctttgtagtt accggttgca gctgcaacat ggtcaccgtt gttatcaact
     7861 tcgttctgac cagcttcgat agaagcagtg ttgtcgttat cgccgttacc gttaatggtc
     7921 aggtgaccat cagagttcat tgcgattaag ccataaccgt agtttgactt ggtagtatcg
     7981 ttggtacggt cgttggtcag gtctgcatca agtacgaagc ggttgctttc gatgttgaac
     8041 acaccagcgt gaatgttacc actggtatca gtagtgctgg tcaggttcag agtatcaacc
     8101 tgcagagcat tcgccgcagt gttaccgaca tccagaatac cgtcattagt cactttgatg
     8161 ttgttggcat acagagcagc atcacctaca gcccaaccag cagaaacttc atcagaaatg
     8221 gttacggtac tgttgtcgac agtcagtgaa tcagttgcaa catgaccatc ttcgttgatg
     8281 ttcagcgcag aaagacctgt cagagagata gagtcagaaa tcagcgttga atcagcaaca
     8341 ttaacagttg aaccactgtt gatgctcaga gtatcaatca gagaagtttt ggtggtatcc
     8401 cactgtgaac cggtattcag ggtcacattg aacagaccgc tctggtatac ttcgttaccg
     8461 atgatgtgat caaattcgct gtattcagag tcgctgtttt ctacaccaac agttgatgaa
     8521 ggccacaggc tgttagctgc gatgtcgatc agagttgcag ttgcttcggt gcctgtactg
     8581 gtatcaataa caaccccgtt gtcattgtaa acaggaccgt aagcatctgg cacaccatca
     8641 tcattggtat caacagttaa gtgcgcagac attgccgcac caacccactt gctgccattg
     8701 ttcagggtca gatccaggcg atcagtacca tcccaaccgt tagtatctac atcaccgtca
     8761 gcatcacgat agctatcacg accatgcggg aagaagttct catcaaagtt acttgagaag
     8821 aatacatcgc ccatcagtgt agaattatcg aatgttgcag tggtttgcat tgcgttatca
     8881 gcagcaggat gtgcaataac ggctaatgcg atatcatctc tattccagtc accgccattg
     8941 taatcgctgg cattgccggt attaccaaac caaccactag taccttcatc agaccatgaa
     9001 ccagaggtca cagtagagtc tttaacagtg atggtgttgt taaacacttc gctgctgtta
     9061 acaccagtgc taaccgtgtt gtcgtcgata tcttcccact cataaccctg agtcaacgta
     9121 atacccgcaa cgtgagagtt attagtgatg gacagatcaa cttcctgatc cagagtaata
     9181 gccgtaccaa tgttgtaaac gtctacaaca tgagtatcag cggcattatt gtaagtgccg
     9241 ttatatgtgt agtgttcgta gttgtcgtcg atcgttgaat tatcaacagt cagcgccaga
     9301 cggtcatggt aataatca
//
LOCUS       DAZGAN020000160         9168 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGAN020000160 DAZGAN020000000
VERSION     DAZGAN020000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.160"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..897)
                     /locus_tag="ACHKYO_RS22630"
                     /old_locus_tag="pgaptmp_004526"
     CDS             complement(<1..897)
                     /locus_tag="ACHKYO_RS22630"
                     /old_locus_tag="pgaptmp_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HGU4431245.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAG"
     gene            1233..2435
                     /gene="nupC"
                     /locus_tag="ACHKYO_RS22635"
                     /old_locus_tag="pgaptmp_004527"
     CDS             1233..2435
                     /gene="nupC"
                     /locus_tag="ACHKYO_RS22635"
                     /old_locus_tag="pgaptmp_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HGU4431246.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(2485..4674)
                     /gene="pdeA"
                     /locus_tag="ACHKYO_RS22640"
                     /old_locus_tag="pgaptmp_004528"
     CDS             complement(2485..4674)
                     /gene="pdeA"
                     /locus_tag="ACHKYO_RS22640"
                     /old_locus_tag="pgaptmp_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGU4431247.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQSVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(4882..4957)
                     /locus_tag="ACHKYO_RS22645"
                     /old_locus_tag="pgaptmp_004529"
     tRNA            complement(4882..4957)
                     /locus_tag="ACHKYO_RS22645"
                     /old_locus_tag="pgaptmp_004529"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4922..4924),aa:Ala,seq:ggc)
     gene            complement(4997..5072)
                     /locus_tag="ACHKYO_RS22650"
                     /old_locus_tag="pgaptmp_004530"
     tRNA            complement(4997..5072)
                     /locus_tag="ACHKYO_RS22650"
                     /old_locus_tag="pgaptmp_004530"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5037..5039),aa:Ala,seq:ggc)
     gene            5293..5652
                     /gene="yfeC"
                     /locus_tag="ACHKYO_RS22655"
                     /old_locus_tag="pgaptmp_004531"
     CDS             5293..5652
                     /gene="yfeC"
                     /locus_tag="ACHKYO_RS22655"
                     /old_locus_tag="pgaptmp_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU4431248.1"
                     /translation="MFKERMIPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            5654..6046
                     /gene="yfeD"
                     /locus_tag="ACHKYO_RS22660"
                     /old_locus_tag="pgaptmp_004532"
     CDS             5654..6046
                     /gene="yfeD"
                     /locus_tag="ACHKYO_RS22660"
                     /old_locus_tag="pgaptmp_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU4431249.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(6098..7513)
                     /gene="gltX"
                     /locus_tag="ACHKYO_RS22665"
                     /old_locus_tag="pgaptmp_004533"
     CDS             complement(6098..7513)
                     /gene="gltX"
                     /locus_tag="ACHKYO_RS22665"
                     /old_locus_tag="pgaptmp_004533"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGU4431250.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            7772..7847
                     /locus_tag="ACHKYO_RS22670"
                     /old_locus_tag="pgaptmp_004534"
     tRNA            7772..7847
                     /locus_tag="ACHKYO_RS22670"
                     /old_locus_tag="pgaptmp_004534"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7805..7807,aa:Val,seq:tac)
     gene            7892..7967
                     /locus_tag="ACHKYO_RS22675"
                     /old_locus_tag="pgaptmp_004535"
     tRNA            7892..7967
                     /locus_tag="ACHKYO_RS22675"
                     /old_locus_tag="pgaptmp_004535"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7925..7927,aa:Val,seq:tac)
     gene            8014..8089
                     /locus_tag="ACHKYO_RS22680"
                     /old_locus_tag="pgaptmp_004536"
     tRNA            8014..8089
                     /locus_tag="ACHKYO_RS22680"
                     /old_locus_tag="pgaptmp_004536"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8047..8049,aa:Val,seq:tac)
     gene            8094..8169
                     /locus_tag="ACHKYO_RS22685"
                     /old_locus_tag="pgaptmp_004537"
     tRNA            8094..8169
                     /locus_tag="ACHKYO_RS22685"
                     /old_locus_tag="pgaptmp_004537"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8127..8129,aa:Lys,seq:ttt)
     gene            complement(8278..9162)
                     /gene="xapR"
                     /locus_tag="ACHKYO_RS22690"
                     /old_locus_tag="pgaptmp_004538"
     CDS             complement(8278..9162)
                     /gene="xapR"
                     /locus_tag="ACHKYO_RS22690"
                     /old_locus_tag="pgaptmp_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HGU4431251.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
BASE COUNT         2413 a         2371 c         2159 g         2225 t
ORIGIN      
        1 tccggcagca accagactta acccaaagac cgttgccgca gcatggctta acagcggttg
       61 cagcgtcaga taagcctcat caagatcggc aacaccagta tgaccagaaa agtggaacgc
      121 cgccgcagct gtagccatca tcgccagatt gacaaaaccg gcaatcgtca tggcgatagc
      181 cacatcccat ttggtggcgg aataacgttg ttgacgcgaa ccgccatgta aatgctgagt
      241 gagcgaagag tgcaaataaa tcacatgcgg cataatcgtc gcccctaaca cgcctgctgc
      301 caggaagacc gcttccgaag taggtaaact cgggatcacc attcctttac ccagctgcgc
      361 caggttaggc tgggaaaaaa tcaactcgac aatgtaagcc gcggcaacaa acaacagtaa
      421 cccaccaatc actttttcca gcggtttttg cccgcgacgt tgcagcatta aaatcaggaa
      481 agtcgcgatc cccgtcagca ccgcgccctg caacaacgag acaccaagaa tgagtttaaa
      541 accgatcgcc gcaccaataa attccgccag gtcggttgcc atcgcaataa tttctgcctg
      601 aacccaatag aaccacacta cgggacgcgg atagtgatcg cgaatctgct ccgccagatt
      661 tttaccggtg gcaatcccta gtttggcaga gaggatctga atcagcatcg ccatcaggtt
      721 ggcccaaacg acaacccaca gtagctgata gccgaagctg gcacccgcct gaatattggt
      781 cgcaaagtta ccgggatcga tataaccaat cgccgcaatg aacgcaggtc ccattaatgc
      841 gagcctcatc ttgcgcgccg cccgtccgct gctactctca acgcgatagt tcgtcatctt
      901 gtgcctctaa aacatagcct ttgctatgtt tcatgctatg ccaaacgaga atgattatca
      961 aattcattta aatgggttgt ggtgatttct ctgatagacc tggattatga ctacgaaaag
     1021 attgatgact tgaatgttat ggttatgttt aatgttagca catttacata actttcagct
     1081 tccatacaca acatagcaga aatgtatgac agatcactat ttttgaagct tgtcacagga
     1141 cgtcattata gtgtgtgtca gatctcgttt tccttaacca tgttacatag aatgtgcacg
     1201 gaaatttaac ctgcctcata tttggagcaa atatggaccg cgtccttcat tttgtactgg
     1261 cacttgccgt tgttgcgatt ctcgcactgc tggtaagcag cgaccgcaaa aaaattcgta
     1321 tccgttatgt tattcaactg cttgttatcg aagtgttact ggcgtggttc ttcctgaact
     1381 ccgacgttgg tttaggcttc gtgaaaggct tctccgaaat gttcgaaaaa ctgctcggat
     1441 ttgccaacga agggactaac ttcgtctttg gtagcatgaa tgatcaaggc ctggcattct
     1501 tcttcctgaa agtgctgtgc ccaatcgtct ttatctctgc actgatcggt attctccagc
     1561 acattcgcgt gttgccggtg attatccgcg caattggttt cctgctctcc aaagtcaacg
     1621 gcatgggcaa actggaatcc tttaacgccg tcagctccct gattctgggt cagtctgaaa
     1681 actttattgc ctataaagat atcctcggca aaatctcccg caatcgtatg tacaccatgg
     1741 cagcaacggc gatgtccacc gtatctatgt ccatcgttgg tgcatacatg accatgctgg
     1801 agccgaaata tgtcgttgcg gcgctggtac tgaacatgtt cagcaccttt atcgtgctgt
     1861 cgctgatcaa cccttaccgt gttgatgcca gtgaagaaaa cattcagatg tccaacctgc
     1921 acgaaggtca gagcttcttc gaaatgctgg gtgaatacat tctggcaggt ttcaaggttg
     1981 ccattatcgt tgccgcgatg ctgatcggct ttatcgccct gatcgccgca ctgaacgcac
     2041 tgtttgctac tgtgactggc tggtttggct acagcatctc cttccagggc atcctgggct
     2101 acatcttcta tccgattgca tgggtgatgg gtgttccttc cagtgaagca ctgcaagtgg
     2161 gcagtatcat ggcgaccaaa ctggtttcca acgagttcgt tgcgatgatg gatctgcaga
     2221 aaattgcttc cacgctctct ccgcgtgctg aaggcatcat ctctgtgttc ctggtttcct
     2281 tcgctaactt ctcttcaatc gggattatcg caggtgcagt taaaggcctg aatgaagagc
     2341 aaggtaacgt ggtttctcgc ttcggtctga aactggttta cggctctacc ctggtgagtg
     2401 tgctgtctgc gtcaatcgca gcactggtgc tgtaaaacca tacataaaaa agccggggat
     2461 cattccccgg cttttttacg cctgttaatc agctaatggc tgcgggcgac caatcaaata
     2521 cccttgcaga tattgcaccc cgagcttatg caatagcgcc tgctgctgtt gcgtctcgac
     2581 aaactccgcg accacactca atgacttcgc tttcgccaga tcggtaattg atctcacaat
     2641 catcgcatcc agcgtgttcg tgacaatatc tttcacaaag acgccatcaa ttttgatgat
     2701 atcagcctgc aaacgcttta accgttcgta gttggcatat ccggtgccaa agtcatcaat
     2761 cgcaatccgg aaaccaaact tatgcagctg ctcgatgttg tacatgctgc tttctgcgtt
     2821 agaaaacgcc tgctcctcgg tgatctcaag aatgaccgac tgcggggaga tgtgataacg
     2881 cttaaacaga cgaataatcc gcccggcaat attcttttgc agcagcgtga gcggcattaa
     2941 attgactgaa aagcgcggtc cttttttgtc gcaagggtgt gttgccagcc acttcaacaa
     3001 ggattccagc acttgcaaat caaaacgcgc gctaaggtta aactgagcaa taaggggcag
     3061 aaacttatcc ggggtcataa tgccgccgtc atatttcagt cgcgcgagga tctcatcata
     3121 accttcaccc tctttgttgc gaattggctg ggcgtagagc agcaaatcac cctgatctaa
     3181 cgccgtgcga atggtattca gcaatagcac ctgtttagtg gtctgcccgg aaaccatctc
     3241 ctctctgcta tccagcgcca gcacatgatg atgtgcgcag gattgctccg ccagccagct
     3301 taactgcccc aacaagggtt gcagggtttc ctgatttcca tcaaaacgcc cccaggcagc
     3361 accatagccc atatccagcc cggtattgtt ccagtgaatt tgccgactat tcaggatgtt
     3421 aaccatatgc tggagtcgcc cttccgtttc cggcccgctc agcactaaca gcagttcact
     3481 acccggcaat tgatacaact tttcgttttc ctgcatcaac ggcagcagcg tacggcaaat
     3541 tgagcggata cagtgaacgc gcatcattaa gccgtaatga cgactcataa actcaagatt
     3601 atcaatgcgc aggcagcaaa aactcttgcc cgcctcttgt tccggcgctt gttccaacgc
     3661 acgaaagttg ggtaggagcg ttaacggatc tgtcagcgcc tgcaaatgcc agcggcgatt
     3721 aagccattca ctgcgatgat aaatgcgcac catatagagc aggcaaacgc taaaggaaat
     3781 cagcaccgca agaataaatg ccagcgaata ttcggtttcc accccttgca aaaagttctg
     3841 gttgtaattc agcaggcaaa gcgttgaaac agcccaggtg agatttaaaa acggatagcg
     3901 aagcttaccg accccgaggg taaagatgat gaagaaaacg ggtaccaagt aaccggcaat
     3961 aaagtcgttt tcataaggtg tgcacaacag aagtagcagc acgcttaacg ctgccagcca
     4021 gcttaaggta aatgcgcgtt tctctttgcc caacgacgga gcgatatccc tgcgccacaa
     4081 tatctgcgca aagtggggac ttacaatcat gcgagtgaga tagtagaaaa gcatgttgta
     4141 aatcagcaca gcggtgaaaa ggcttagcaa atcaacgacc gtgaaaatgg catccgcatc
     4201 gccgaaaaag gtggatatct ttagcgggaa atcaaagaag ctccccacaa gatacatgct
     4261 gcatttgatg ccgatcggcg tcaccaaacc aagccagaac agacgttgcc agacatatcg
     4321 gctggtcaat ccataacgcc aacgcgtccc cagctgccag cgtaatattg cgcaggcaca
     4381 caacacgaca aacgtctggc taccgagcat gaccgcagtc tgtaaaaaag agagatgaaa
     4441 gttccacaga ttggtgcaaa acatcccgca taaaacggga acgacaccgc gccagccaaa
     4501 gataaacaag attgacagca ttacgcacag tggcatccac gccagaaaga tataactgga
     4561 atggataatg gcaagtggcg aaataaaacg ggatagctga ataagtacca cggtgagcgt
     4621 aaacgctagt gtgaatatct tgatattttt tatcaggtta tgctccacaa acatgagtac
     4681 actactctca ctgatcgggg ttttcaggca cacaaataat aagtttattc gcatcgcgaa
     4741 gcaaacaggg gccagcctgt tgccgatttt tcatccaatg atgctttcac caggcggcgc
     4801 taacccggac gtagactgta ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct
     4861 ttgcagcgag ggttggaaat ttggtggagc taagcgggat cgaaccgctg acctcttgca
     4921 tgccatgcaa gcgctctccc agctgagcta tagccccacg atgcgtttac gtaccaaatt
     4981 tgctgggtgc aaaatttggt ggagctaagc gggatcgaac cgctgacctc ttgcatgcca
     5041 tgcaagcgct ctcccagctg agctatagcc ccgtcacgta aagcttgtcg agttgacggg
     5101 cggcatcata tgaattccgc ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat
     5161 cgctgaaaaa tcaggcaaat gaacaatttt ggaaaaccgc tcgcattcag tagttattca
     5221 tgtcacggtt tcctgtaaag tggtgttata aaatgaacta ctaatagacc cacatacatt
     5281 cagggaattg ttatgttcaa ggagaggatg ataccagatg aacttgccag actgaccggc
     5341 tatagccgcc agaccattaa taaatgggta cgcaaggaag gctggacaac gtcaccaaaa
     5401 cctggcgtcc agggtggcaa agcccgactg gttcacgtca atgaacaggt tcgtgaatat
     5461 attcgcaatg ctgaacgtcc agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg
     5521 ccgcttgaag tattgctggt gacactggcg aaagagatga cgccagttga acaaaaacag
     5581 tttacatcct tgcttctgcg ggaagggatt atcggattgt tacaacgctt agggattcgc
     5641 gatagcaaat aatatgaaaa gattacgcaa caaaatgacc accgaagaac tggctgaatg
     5701 cctcggtgtg gctaaacaaa ctgttaaccg ttggatcaga gaaaaaggct ggaaaacgga
     5761 aaaatttccc ggcgtgaaag gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg
     5821 cgagtttatt cagaacacgc cagccttcca taacacacca atgttgatgg aagctgaaga
     5881 gagaattgct gaatacgctc ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc
     5941 gatagataat atgacagaca ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg
     6001 aattcgcaat ttcctcgccc gtctcgatat tgacgaatca gcataaataa aaaacggcag
     6061 gatattatct cctgccgttt atctttttac acgctaatta ctgctgattt tcgcgttcag
     6121 caataaaatc cagcgctttg ttgatacgct cgatactgcg ggtcttacca attgcgtgaa
     6181 cggtaacatc cagtgctgga gactgccccg caccggttac ggcgacacgc agcggcatac
     6241 caactttacc catacccact tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt
     6301 tttcagcggt ccagtcagta atcgcggcca gtttgtcacg aaccacttcc agcggctgac
     6361 gcgctaccgg acgcagatgt tttttcgccg catcggcatc gaactcagca aaatcttcgt
     6421 agaaataacg gcagctctgt gccatctctt tcagcgtctt gcagcgttcg cccagcagtt
     6481 tcaccagatc agccagctgc gggccgttac gggtatcgat attttcctgc tcaatgtgcc
     6541 actgtaagtg agtagcaaca tactccggcg gcagcgcgtt aatgtagtga tggttcagcc
     6601 acagcagctt gtcggtgttg aacgcactgg cagatttgct gacggcattc aaagtgaagt
     6661 atttgatcat ctcttcacga gtgaagattt cctgatcgcc gtgggaccag cccagacgca
     6721 ccagatagtt cagcagtgct tctggcaaat aaccgtcatc acgatactgc attacgctga
     6781 ctgccccgtg acgtttggac agttttttac cgtcatcgcc attgatcata gaaacgtgcg
     6841 cgtaaaccgg caccggcgct ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt
     6901 tgatatggtc ttcgccacgg ataacgtggg tgatttccat atcccagtca tcgacaacca
     6961 cacagaagtt ataggttggg gaaccatcgg tacggcggat aatcagatca tccagctcct
     7021 ggttgctgaa ctcgatcgga ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg
     7081 gattagcaaa acgcacgaca cacggttcat catcagcatg atgctcatgg ctgtggcggc
     7141 agcgaccgtc ataacgcggc ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca
     7201 ggcgctcttt agagcaatag catttataag cagtgccctc ttccagcatc tgatcgatca
     7261 ccgcattgta gcgatcaaaa cgtttggtct ggtagtacgg accttcatcc cactccaggc
     7321 tcagccagtt catgccatcc ataatggctt cgatagcttc cggcgtggaa cgctcaagat
     7381 cggtgtcttc aatacgcagc acgaactcac cgccgtggtt acgtgcaaaa agccaggagt
     7441 aaagagcagt acgcgcgccg ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag
     7501 ttttgatttt catgaaatgg ccttacgttt agaaagatgc cgacaaccgg caaatcctgg
     7561 aaaaattgag tgggcgatat tctatcactc ccgcctgatt cctcaatgta gttcgggttt
     7621 ctaccttacg cattattgtt ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca
     7681 tcttgcttaa atttacgacg aacgaacaat ttctttagaa aatgcgttga ctcattttga
     7741 actctcccta taatgcgact ccacacagcg ggggtgatta gctcagctgg gagagcacct
     7801 cccttacaag gagggggtcg gcggttcgat cccgtcatca cccaccaact actttatgta
     7861 gtctccgccg tgtagcaaga aattgagaag tgggtgatta gctcagctgg gagagcacct
     7921 cccttacaag gagggggtcg gcggttcgat cccgtcatca cccaccactt tctcgccagc
     7981 taaatttctt gtaaaaatgt gaagtaccta agtgggtgat tagctcagct gggagagcac
     8041 ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccac ttcgggtcgt
     8101 tagctcagtt ggtagagcag ttgactttta atcaattggt cgcaggttcg aatcctgcac
     8161 gacccaccaa tgtaaaaaag cgccctaaag gcgctttttt gctatctgcg atttgcgaaa
     8221 ttgcctgatg cgcttcactt agcagacgac tatttccggc aattcctgtc tcctcaccta
     8281 ctgtgtcaat gcagccaaca gcttaaccat cgcgggcgtc acctgctgtg tttcataaac
     8341 aatatataaa tctgcaggga tgcgctgttt gagcggacgg aaaatgacac ctggccagtt
     8401 catttgtgcg tagctgtccg ctatcaatgt gataccaata cccatactga ccatggcgag
     8461 taccgtttgc ggttcattaa cttcgcgaat aacaaccggt gaaaatccca cctgctggca
     8521 aactcgctgc aaaaaatccc agtcagtgta cacgggcggc attgtaacaa aatactcgtc
     8581 acgtagcgct tccagcggaa cggtggaaaa tgatgagaga tgatgctctt caggcatcgc
     8641 caccagaaac gccgattcat gcaaccgtaa gctggtaaaa cctgtcggtg gttctgtcgc
     8701 cattcgccag atcccggcat cgagttcacg tcgttccagc agggccattt gcatcgcggg
     8761 catcttttcg cgaaaaagaa cttcaacgtt aggattttcc ctgaggaatc gccgcataac
     8821 cgggcgcatc cgtccccaca tagccgttcc cactacgccg agttcaatcc gccctgcttc
     8881 tccccgacct atttgttcaa cccgagccaa tacattatta gcattcacca gcaatcgacg
     8941 cgattcttcc atcaagattt tgcccgcgtg tgtcagtacg acgctgcgcg aatggcgaat
     9001 aaaaagctgc gtgccgagtt gattttccag ctctttaata tgaatactga gcggaggctg
     9061 agacatattt aaacgcgctg ctgcgcggcc aaaatgcaac tcttccgcta cggcaagaaa
     9121 ataacggagc aacttaagat ctgttctgta tacgcgttcc ataattaa
//
LOCUS       DAZGAN020000161         9132 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGAN020000161 DAZGAN020000000
VERSION     DAZGAN020000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.161"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            117..719
                     /gene="ais"
                     /locus_tag="ACHKYO_RS22695"
                     /old_locus_tag="pgaptmp_004539"
     CDS             117..719
                     /gene="ais"
                     /locus_tag="ACHKYO_RS22695"
                     /old_locus_tag="pgaptmp_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HGU4431252.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(758..1183)
                     /gene="nudI"
                     /locus_tag="ACHKYO_RS22700"
                     /old_locus_tag="pgaptmp_004540"
                     /pseudo
     CDS             complement(758..1183)
                     /gene="nudI"
                     /locus_tag="ACHKYO_RS22700"
                     /old_locus_tag="pgaptmp_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
     gene            1462..2004
                     /gene="yfaZ"
                     /locus_tag="ACHKYO_RS22705"
                     /old_locus_tag="pgaptmp_004541"
     CDS             1462..2004
                     /gene="yfaZ"
                     /locus_tag="ACHKYO_RS22705"
                     /old_locus_tag="pgaptmp_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HGU4431253.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2104..3306
                     /gene="yfaY"
                     /locus_tag="ACHKYO_RS22710"
                     /old_locus_tag="pgaptmp_004542"
     CDS             2104..3306
                     /gene="yfaY"
                     /locus_tag="ACHKYO_RS22710"
                     /old_locus_tag="pgaptmp_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HGU4431254.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            3370..3831
                     /locus_tag="ACHKYO_RS22715"
                     /old_locus_tag="pgaptmp_004543"
                     /pseudo
     CDS             3370..3831
                     /locus_tag="ACHKYO_RS22715"
                     /old_locus_tag="pgaptmp_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3872..4774)
                     /gene="rpnE"
                     /locus_tag="ACHKYO_RS22720"
                     /old_locus_tag="pgaptmp_004544"
     CDS             complement(3872..4774)
                     /gene="rpnE"
                     /locus_tag="ACHKYO_RS22720"
                     /old_locus_tag="pgaptmp_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HGU4431255.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRQLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLGRQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(4968..6158)
                     /gene="glpC"
                     /locus_tag="ACHKYO_RS22725"
                     /old_locus_tag="pgaptmp_004545"
     CDS             complement(4968..6158)
                     /gene="glpC"
                     /locus_tag="ACHKYO_RS22725"
                     /old_locus_tag="pgaptmp_004545"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HGU4431256.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6155..7414)
                     /gene="glpB"
                     /locus_tag="ACHKYO_RS22730"
                     /old_locus_tag="pgaptmp_004546"
     CDS             complement(6155..7414)
                     /gene="glpB"
                     /locus_tag="ACHKYO_RS22730"
                     /old_locus_tag="pgaptmp_004546"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HGU4431257.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALITVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7404..9032)
                     /gene="glpA"
                     /locus_tag="ACHKYO_RS22735"
                     /old_locus_tag="pgaptmp_004547"
     CDS             complement(7404..9032)
                     /gene="glpA"
                     /locus_tag="ACHKYO_RS22735"
                     /old_locus_tag="pgaptmp_004547"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HGU4431258.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
BASE COUNT         2118 a         2330 c         2432 g         2252 t
ORIGIN      
        1 gcattttgcc gacatcgtag gatttttaat attacaccaa ctgctaatta tcgccagaaa
       61 tgtcgctcaa ttttacggta attgtctggt tgcgcttgtc tataggtgga gtttacgtgt
      121 tagctttttg ccgctcatca ttgaagtcaa aaaaatattt catcatttta ctggcgctcg
      181 ctgcaattgc cggactgggt actcatgccg cctggagtag caatggtttg ccacgtatcg
      241 acaataaaac attggccaga ctggcacagc agcacccggt tgtcgttttg tttcgccatg
      301 ctgaacgttg cgatcgttca accaatcaat gcttgtcaga taaaacaggt attacggtta
      361 aaggtaccca ggatgcccgt gaactgggca acgcttttag cgctgacatc cctgatttcg
      421 atctttattc cagtaatacc gtccggacca ttcagtcggc gacctggttt tccgcgggta
      481 aaaagctgac ggtagataaa cgacttcttc agtgcggtaa tgagatttat agtgcaatta
      541 aggacttaca aagcaaagcg cctgataaaa atatcgttat tttcacccat aatcattgcc
      601 tgacatatat tgctaaaaat aagcgtgatg cgacatttaa acctgattat ctggatggtt
      661 tagtcatgca tgtggaaaaa ggcaaagttt atctggatgg ggaattcgtt aaccactaaa
      721 ttttcaatct gacagccagt gatggctgtc atcattgtta aagaagacct ttcaaacgta
      781 acgtttttcg ggtggcaaca tttaaatcat aatgcaccag atcttcaggt tttacccacg
      841 cgtagtcctg aaactcttcg tttattttca cttctcggtt ggcagaaacg cagtcaaaaa
      901 tcaggtaaat catataaatc tcttccttgc gaccatctgc atacgtcttg gtgcgaatat
      961 catcgctgaa ggtccacggc gtgatttctg tcaaaagcag ctattctccc agttcttcgc
     1021 gaatttcgcg gcgtagtgcc tcttcaattc gttcgccaga ctccacgccg ccacccgaaa
     1081 gcgcccattg accggggaaa acgccgcgat cgtcggccat tttacacagc aaataagcac
     1141 catcattttg aatcaaaggg catacaatag tccgttgtcg cataggggta ttccttataa
     1201 ataaaaagaa accatgagaa tacggtaact cagaaatatg tgaaagtgac agtaatctct
     1261 tttgcaatga taactgcact tattattatt tttgtgtgta tcggtaaagt gtgtttattt
     1321 tttcggcgtt taattattta agaagtattt agttacaata agtgactaga tccctttttt
     1381 cgctcattga atcctctgtg ccaggcagta caatcccgca gctttgggaa ttttgattaa
     1441 ttgcttgtgg gaaaggggat aatgaaaaaa attgcgctag caggtctggc cggaatgctg
     1501 ttggtttctg catcggtcaa tgcaatgagc atcagcggcc aggcgggtaa agaatacacc
     1561 aatattggtg tcggttttgg gactgaatcg acgggcctgg ctttaagcgg taactggaca
     1621 cataacgacg acgacggtga cgtcgcgggc gtggggctgg ggttgaatct gcctctcggg
     1681 ccgttaatgg cgaccgttgg cggaaaaggc gtttacacca acccgaatta cggcgatgaa
     1741 ggttatgccg cagcggtagg aggtggtttg cagtggaaaa ttggcaacag cttccgtttg
     1801 tttggcgagt attactactc tccggattcg ctctccagcg gtattcaaag ttatgaggaa
     1861 gcgaatgctg gcgcgcgtta caccattatg cgtccagtca gtattgaggc gggttatcgc
     1921 tacctgaatc tgtcgggtaa agacggtaac cgcgacaacg ccgtggctga cggcccgtat
     1981 gttggggtta acgccagttt ctgatccctt ccggcgcggc attctgtcgc gccattcagt
     2041 ttcttactac gccttatctc cctttgcgtt atagtgattc gataacaaaa gcaggagata
     2101 accatgttaa aagtggaaat gctatccacc ggggatgaag tgttacacgg gcaaatcgtt
     2161 gacactaacg ctgcctggct ggccgatttt ttctttcatc aggggctgcc attatctcgc
     2221 cgcaatacgg tgggggataa ccttgatgac ttagtcacca ttcttcgcga acgtagtcag
     2281 cacgccgatg tgctgatcgt taacggcggg ctgggaccga ccagcgatga tttaagcgca
     2341 ctcgccgctg cgacagcaaa aggtgaaggc ctggtgctgc atgaagcctg gctcaaagag
     2401 atggaacgct atttccacga acgtggacga gtaatggcac cgagcaaccg taaacaagcg
     2461 gagctgcctg ccagtgctga atttatcaat aacccggtag gcaccgcctg tggttttgcc
     2521 gtgcagctta atcgttgcct gatgttcttt actcccggcg taccgtcaga atttaaggtg
     2581 atggtcgagc acgaaatcct gccgcgtctg cgcgagcgtt tttctttacc acagccgccg
     2641 gtttgtctgc gtttaactac ctttggtcgt tcggaaagcg atctggcaca aagcctggac
     2701 actctacaac tgccgccggg cgtaacaatg ggctatcgct cttcaatgcc gattatcgaa
     2761 ctgaaactca ccggaccggc aagcgagcaa caggcgatgg aaaaactgtg gctggacgtt
     2821 aaacgtgttg ccggacagag cgtgattttc gaaggcaccg aaggactgcc cgcgcagatc
     2881 agtcgcgaat tgcaaaaccg ccagttcagc ctgacgttga gcgagcaatt caccggtggt
     2941 ttattggctt tgcaactttc tcgcgcaggt gctccattgc tggcgtgtga agtggttcct
     3001 tcacaggaag aaaccctggc gcaaactgcg cactggatta cagaacggcg ggccaaccat
     3061 tttgccgggc tggcactggc tgtttcgggt ttcgagaacg aacatctcaa ctttgcgcta
     3121 gccacaccag acggcacttt cgctctgcgt gtgcgtttca gcactacgcg ctacagcctg
     3181 gctatccgtc aggaagtgtg cgcaatgatg gcactgaata tgctgcgccg ttggttaaac
     3241 ggccaggata tcgccagtga gcatggctgg attgaggtta ttgagtccat gaccttatct
     3301 gtctgattcc gccactctgt gccggtttac tgcaccggca cattccccct ccggcaaaac
     3361 ttgtccaacc ctccctacgg tgagcgtggt gttggggcca gtgtggcacg ggctgcgcgc
     3421 tggggacgca ttgagaatta catggcgcaa gttaacgatt cactttgtct gttggtgcag
     3481 gtggaaagta aaacggcact ggataacctg gacgagatcc tcgacgtcga agggattgat
     3541 ggcgtgttta ttggaccagc ggatctttct gcgtcgttgg gctacccgga taacgccggg
     3601 caaccggaag tgcagcgaat tattgaaacc agcattcggc ggatccgtgc tgcgggtaaa
     3661 gcggctggtt ttctggctgt ggctcctgat atggcgcagc aatgcctggc gtggggagcg
     3721 aactttgtcg ctgttggcgt tgacacgatg ctctacagcg atgccctgga tcaacgactg
     3781 gcgatgttta aatcaggcaa aaatgggcca cgcataaaag gtagttattg atatcaaagg
     3841 cccatgggga tcggctgtgg gcctgtgtta attagtggtt attcgctgcc agatcggctt
     3901 cgcttagctg ggtggccgcg agcacctggt cacgatcaat gccctgttcc agcatgcgca
     3961 aagcaatttt aatggcttgt tcatgcatac cttcctgcca gccaatttga tgcccttcct
     4021 gccgtagccg ttcagcaata gtcattaatc tctccttttg tagtggtgaa cgttcggcaa
     4081 tctcctcaat aaaacgggtg aaacgggagg tatcgccgca ttgcagcaga taattaaaca
     4141 gtgtttgtag ctggctgtca ttagtgaagc ctctaaccaa aagcgtggtg atcctgtcaa
     4201 ccatgccgat taaatcgcgg tcgcgaatat gcttttgaat cagttccagc agagctatac
     4261 gccgatgttg catgatctca tcgtcaggta cgatggtgat atccaccaac ggaaaagctt
     4321 cggtgtacaa ctgccggcca agttgcggat cgtcaaactc atccagccag ttaagtgagt
     4381 acgggtaggg cgatgtttcg ccatgataaa acagcaatgg caccaccagc ggaactctgt
     4441 catagccttt atccagctga cgctgcatgg cggcagtggc atagcgcatt agccgaaaag
     4501 ccatattctt ttctgcagag ctttgatgtt caatcacgca gtagatatag ccgtcaccgt
     4561 cgctggtttc cacggaccac aaaacatccg agtagtaagc gcgtaaactt ttttcaataa
     4621 aactagtggg ttccaggcgt aaggtttgca ggttgcaaag cttgcgcagt ggttctggta
     4681 aatgtatttc gagaaaatcc cgtgcggttt cgggtgtgaa cataaaggtt ttaaacaccg
     4741 catcatgcgg ggaggatgtt gttgattcgg tcatggttgt ccgtcacctg atatttatga
     4801 tgacgcgaca ttacgtctat cccgggcctt tgacatctgt cagtttcagc ctttgcgaga
     4861 cattttcaga ggaattttca aagcgttgca acgcatacat ggtgcgtgga atcgcgctcg
     4921 caaaaatgcc cgataagcaa tgtgcttacc gggcatcaaa aggtcggtta agccagcgcc
     4981 tgggccagta gcgtaatcgg atgttcgcag cgaaggctcg tggacatctc aatctgccat
     5041 ttgcaggttt cgcagtcggt gatgaccaga tccgcgccgc tctcttctat ctggcggaac
     5101 agtggcgcac cgatggcttg tgaggtgggg tagttctctt ttttgaaacc gtaagtaccc
     5161 gcaataccgc agcactggga atccagcacc gttaactcaa gccccgggat tttacgcaac
     5221 agctccaggg tgtagagcgt ccagcccatt ttttccatat ggcacggagt gtgataaacc
     5281 actttcagcg gcagcggttt cagcggtaac gttttgcctt cgtccagctt gcgccacagc
     5341 cagcgggttg ccagttcgat atgatcgcgc aagcctttgt tgtcgacatt cagcacttcc
     5401 gggtattcgt cgcgcagggc aaatgtacag gttgaggagg tggcaatcac cggaatgcct
     5461 tttactccca cagcttcgcg gatcgactct acattcgtaa ttgcctgttt gcgtgcttta
     5521 tcggtaaagc cgttggcgat tagcggtacg ccgcagcatt tttctttgct gagcagttgt
     5581 acaccggtac ccattgcgtt gagcacttta attaaatctt tacctaactg cggatggttg
     5641 tagttaacga agcagccgtg aaagaaagcg acctggtctt tatattgtgc ttgctgagcc
     5701 gctatgctgc gataccagcg acggaacgtg ccgaaggagt atttcggtag cgtgcggcga
     5761 tgatcgattt ttaacgccgc atcaagcagc tgccgcaccg gtttcagcga ggtagcggtg
     5821 ttgacgattg gtgcgaacgg cgtggaaacg ctacccatca ggtcggtatg actcaacaca
     5881 aaattacgca gcgacgggcg cgtggtgtca tattttgccc gcgcgcgctg gataatatcg
     5941 ccaatcttca catcggacgg gcaggcgact tcacaacgtt tgcagttgat gcaatatttc
     6001 agcgcctcgt catacagtgc gccatctttc aaacgcagac gctcgccatc cggcccggct
     6061 tgttttggcc ctggataacc gggattcacc cggctcaccg ggcaggcggt ggtgcagacg
     6121 gtgcacttaa tgcagttttc gaagctggtg tcgttcattg ttggcctcct gcgcgttggg
     6181 caatctgttg agcggcatgt aaagcactga cggcacaaac accgccgccg catccctggg
     6241 cgatgggatc aaatccgccc agcaccgaac cgatggcaaa caggttctca atggtttgcc
     6301 ctgcctgcga cgggcgtagc gtctcatcag tggttacacc gaactgctgc cacggttgcg
     6361 gcgcaaaaaa atcgccctta taccattcac cccgcgtggc ggtttgtagc acatcaaggc
     6421 cgagaatcgg ctcgcgaatg ccgttacgtt ccgctaccag tccgccacta aagaaactgc
     6481 cgctggcgag aaccgcgaaa cgtggacgta gcggaatatc ggcgtgattg cgggtccaga
     6541 tttcattcac tacgccattt ttacaggtca cttttttcac ttcatcgccc ggcatccaca
     6601 cgccaccctg gcgcacaaac tggcgctgta actggttttg cagacgaatg cccagcacgg
     6661 aaggcggcag cgttggcaaa agcatcagtg aacaaggtag tttttcattc aaccaacgcc
     6721 acagtttgtc atcggccagg ccgaagcagg cgggcatcag gatcatttcg caggtattgg
     6781 cgacagtaat aagcgcatca agtaacagcg gccagttttc ttcattatca aggaaacggg
     6841 cgatattcac cgcgcgaaat tcggtggcgt tattgcgcag gacatccagt tccggcagct
     6901 ctatttctgc ggtttcaacc gaaagatcca gttcgcgcaa cgaagccgcc gcaagatgcg
     6961 cctggaaatc catcagcccg cttatcccca ctacacatat cttcttcgcg ggtagcggcc
     7021 agacgggaac ttctggcgaa cttaaccagg ttgcacgcaa agtgccgagc ggggtaacgc
     7081 gctgatgggc cagttccacg ctgccctgca attgcgcacc gctttcagcg atcaatgcct
     7141 gcgcttggca agcgagatcg agcacgcgtt gtggttcgag aagactgtaa ggatgggctg
     7201 gtgcctgctg acgcaaagat tccagtccac tgtgaatgtc tgtcaccggt tgaccatcag
     7261 gcagatggct cagcaaatcc agcgaaccgg atgagaaatg cagcgcgctt tgaccacgcg
     7321 tgacaatggc acagcgcagg ccgtgttttt gcagttgcag gccacagagt aatccggcga
     7381 ggccgccgcc cataatgaca gtatcaaagc gcatctttct gctccttctc cagaccacac
     7441 aatccctgat aaacccagcg ggtaaattcg ctttcgcgca gtgcatctcc ccaggcgatg
     7501 ggttgcacgc ctttccagcg ttcgttaagg aaggtggaaa gttgctcgat agattgcgcg
     7561 gacgtcgtga cgttaaaacg ttgcagcagt ccggcagcgc ggcaggcgca gagttcgccc
     7621 tggcaggtac ccatccccac acgggtacga cggcgtaaat ccagcaggct attaacgttt
     7681 aaattttcta ccgcgtactg aacttcaccc gcagttaccg cttcgcactc acataccagg
     7741 ctacgatgca gacggccttc gctcagccag gcaggcgtgc gatcgccatg acgataaacc
     7801 gcagaaccgc gcagcggggc aggcagggag atgactttac gcaaggtaac ttcagcgggt
     7861 tcttgtgaac caggcagtgc cagatcggca gtcgtacagg ggcgcgtgtt gcccagtttg
     7921 cggcataccg cgtcggtagc ccattcagcc atcagccgat aggtcatcag tttgccaccg
     7981 gtgatggtga taaatccgtc cagaccatcg cgttcagcat ggtcgagcag cacgatgcca
     8041 cggctgacgt tacgtccgct cgggtcgtca tcgctggcaa ccagcgggcg cacgccagaa
     8101 taggcccgca aaatgcgcgt tttcgccatc acgggggcca gtttttcccc ttcacgcagc
     8161 agaatatcaa cctcttctgc cgtcactcga ttatcgtcaa tctcgttgta atcaatacgt
     8221 aaagaggtgg tgccaatcag cgaaatggta tcgccaggca ccagaatatc ggcgtcggaa
     8281 ggtttacggc agcggttgat cacatgctgg ttaatgcggt gatccatgat cagcagcgat
     8341 cctttcgccg ggaacatgcg aatgcgcaga tcggcatatt cggcaatgtg ttgcccccag
     8401 atcccagcgg cattaaccac gacaggtgca tgaagggcct gagtttcgcc ggtgagatga
     8461 ttacgtacac gaacaccgca caccgtcgcg ccttcacgaa tcagccccgt gacttcatga
     8521 gcggtaagga taacggcacc gtgttctttg gcatccagca tgtttgctgc ggtcagacga
     8581 aatggatcaa cggtgccatc cggaactttc accgcgccaa tcagtgccgg gttaacggca
     8641 ggttcgataa tgcgcgcttg ctgcgggtct atagcttctg cgctgatccc tgcttcttcg
     8701 caggcgcgaa taaaagtggc ctggaatgag aggtcatctt ccggcagggt gataaacagg
     8761 ccgttggttg gttcaacgca gtgacgtgca atgcgtttca ggatctggtt ttcactaatg
     8821 cattcgcggg ccgattccgc atcggttacc gcatagcgcg caccgctgtg cagcaggccg
     8881 tggttacgcc cggtggcacc ggttgcgatg tcgtggcgct caaccaaaat cacgcgcagc
     8941 ccgcgcaggg cacagtcgcg ggcaatcccg gctcccgttg cgccgccacc aatgataatc
     9001 acgtcacttg attgcgagtc gcgagttttc attgttatcc ctctgaagtt cgttttttac
     9061 catttagcca tagtaaaaac atgaattgtt tgatttcgcg catattcgct cataattcga
     9121 aagtgaaacg tg
//
LOCUS       DAZGAN020000162         9115 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGAN020000162 DAZGAN020000000
VERSION     DAZGAN020000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.162"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(2..544)
                     /gene="ecpR"
                     /locus_tag="ACHKYO_RS22740"
                     /old_locus_tag="pgaptmp_004548"
                     /gene_synonym="matA"
     CDS             complement(2..544)
                     /gene="ecpR"
                     /locus_tag="ACHKYO_RS22740"
                     /old_locus_tag="pgaptmp_004548"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HGU4431259.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            1362..1595
                     /gene="ykgL"
                     /locus_tag="ACHKYO_RS22745"
                     /old_locus_tag="pgaptmp_004549"
     CDS             1362..1595
                     /gene="ykgL"
                     /locus_tag="ACHKYO_RS22745"
                     /old_locus_tag="pgaptmp_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HGU4431260.1"
                     /translation="MKTQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSER
                     NVRQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(1630..1770)
                     /gene="ykgO"
                     /locus_tag="ACHKYO_RS22750"
                     /old_locus_tag="pgaptmp_004550"
     CDS             complement(1630..1770)
                     /gene="ykgO"
                     /locus_tag="ACHKYO_RS22750"
                     /old_locus_tag="pgaptmp_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HGU4431261.1"
                     /translation="MKVLNSLRTAKERHQDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1770..2033)
                     /gene="ykgM"
                     /locus_tag="ACHKYO_RS22755"
                     /old_locus_tag="pgaptmp_004551"
     CDS             complement(1770..2033)
                     /gene="ykgM"
                     /locus_tag="ACHKYO_RS22755"
                     /old_locus_tag="pgaptmp_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU4431262.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(2396..2497)
                     /gene="ykgR"
                     /locus_tag="ACHKYO_RS22760"
                     /old_locus_tag="pgaptmp_004552"
     CDS             complement(2396..2497)
                     /gene="ykgR"
                     /locus_tag="ACHKYO_RS22760"
                     /old_locus_tag="pgaptmp_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HGU4431263.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2971..3060)
                     /locus_tag="ACHKYO_RS22765"
                     /old_locus_tag="pgaptmp_004553"
                     /pseudo
     CDS             complement(2971..3060)
                     /locus_tag="ACHKYO_RS22765"
                     /old_locus_tag="pgaptmp_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3613..7866
                     /gene="fdeC"
                     /locus_tag="ACHKYO_RS22770"
                     /old_locus_tag="pgaptmp_004554"
     CDS             3613..7866
                     /gene="fdeC"
                     /locus_tag="ACHKYO_RS22770"
                     /old_locus_tag="pgaptmp_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HGU4431264.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLAEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDTDGNPVTGEASRLR
                     FVPQDTNGVTIGTISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGSWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIGDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISNIE
                     VIGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTIVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLAVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKIIRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMK"
     gene            complement(7988..8845)
                     /locus_tag="ACHKYO_RS22775"
                     /old_locus_tag="pgaptmp_004555"
     CDS             complement(7988..8845)
                     /locus_tag="ACHKYO_RS22775"
                     /old_locus_tag="pgaptmp_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4431265.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMAKEWTINRG
                     LYASFRYEGEWERYPEWSRNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
BASE COUNT         2537 a         1950 c         2212 g         2416 t
ORIGIN      
        1 tttactgaac caacttatat atttttgagt acagcttggc ctcagcatta cgccgatggg
       61 tatacactgt cttcacacta caattttcaa ttctggcaat cgatttaggt tgcattccct
      121 gggccgtcat gcggataatt tccatttccc ggtcagtgat tttatctttc ttaatatctt
      181 ttctcaggaa cctgccatta atcacatagg ccagttcttt tctaatatca cgactcagtc
      241 ctgcgtatac cacgccccta atattgctgt tgtaatacca ataatttgct aaggcttctg
      301 actttctggc cgcaatcaat acgacctgtt tcccctttac cgcggataac cattcattat
      361 cacggtttat aaattcagat aaagaatccg tattgagatt gataaacacg aaatcttttc
      421 taattttctc caacgataga taaattaatc tgtcgatatc cacaatcagt tcagatagtc
      481 ctttatagaa gtaggcgtca tggggagacc agatgtattt atcagaacgg ttttgacatt
      541 ccatatggtt tttaacctca tagtccctgc tgtaatcact ttgccatgtc actactttcc
      601 aaacctgtaa tttttacagg tgcattcagt ttccttagta aaccggaagt aaataagata
      661 cgtagtacat atcaataaag gttaaacgag attaagttta attattttcg attgggctta
      721 aatttatgtt tttttcccga tgagtgtcaa tatgtgaacg gaaaaggaat tcaatggtta
      781 agtcaatatt tagaaaaact gaaaatattg cttttttgat aagaagaaaa agacatttgg
      841 tttcataagg ttattggcaa tataacgttc aattcttact tgcattttat attggtcagg
      901 aactttgccc tgaagtgtta ggaatagtct taatgcgctg tgtgatggac tagtaaaaaa
      961 acaagtttta agggtaaggg atagaatata aaactataat gagctgtcat tgaagatgat
     1021 ttgccaatca gtaagaaatt ttaatggatt aacatgccgc acaaaatggt tataggtgtt
     1081 cattacaata agtgatggaa aattgaacgg gcgtatgtac gtcatctgtt cggtaagacc
     1141 cccaagagcc agattaagtg aagttaaacg aaggctgcca gctgtcacag gaacgatggc
     1201 ctctttgtca gggcactgag tgcagttatc tgtgcgcgtt actctaactg gcactcgaac
     1261 gtcggcgatg ggcttaccgg tggatcaaac tgttcctgtg gcgtaaacgt ttttatgtta
     1321 gtaccaacag atttaccaca tttatcatct taacagtttg agtgaaaacg caggctgcgc
     1381 cgcaaaggga ggagactgag tggcgtgttc agtctaaaag agggttgatg ccagcataca
     1441 ggggcgaagc tggtcaacaa gtcaacatta agataatgga atattcggag agaaacgtca
     1501 ggcagttggc ctcaaatgaa caggaagaat atatccccag gaaaattaat gttggtgtta
     1561 taaacacacc aacattaatc cgatctgatt attaatcaga caaatatcgt taattcgtcg
     1621 aattttgaat caacgttttt tcttacgacc ctgaacggcc ttaaaacgtg gattagattt
     1681 acaaatcaca tacaaccgtc cttttcgctt cacaatctga cagtcttgat ggcgttcttt
     1741 tgcggtacgc agagagttaa gaactttcat tacgccccct ttttcgtgct aacaaaacga
     1801 ccaaaacgtt gggtgaaccg tgcaacattt ccttctgatg ccactgttct cagcttccct
     1861 gtatagaacg ggtgcgattt cgaagagaca tcaattgtta cgtacggata cgttacgcca
     1921 tccagctcaa tctcacggtc tgttttgata gtcgagccga ttttaaagta ctcatcaaca
     1981 ctggtgtcgt ggaacaccac agtacgatac tcaggatgga tattgggctt catcattttt
     2041 acctgttatg ttataacata accataaaga tacatgctca tccggatcat ttcaacaccc
     2101 aatccctgtg actctttgtg gatgtggaat tttcgcaacg gggaactctg gcattgttag
     2161 cagccattgg tgacgctccc ataaacgcct ggatgagtga ttgatgatgg ttttgctgag
     2221 agtatggatt tttcgacaat ggaggttatc tccactttac tggccgatga tttatcgcag
     2281 tatgattgat tacctgacag cgtgtattcc tctcccacgg atataataat tacttacaaa
     2341 aagggagagg atgcatattt taaatatcac tgaagtgaac agtttattgc cgttattaat
     2401 agaaatggag aaataaatag gcgtattcta caattgcgac aaaaacaacg atattaatca
     2461 gtttatgact gatttgttgt actttattct ctttcattgg tacttcctcg ctttaaaaaa
     2521 gagcgcactt cgtaagtgcc cttatataaa taacgagttt ggtcaaccaa ttttttgaca
     2581 tgtatcacaa atttgaatag atgtattaca tcggctatct tttattgcac caacgtcatt
     2641 gatatatgtc gcctgaagtc agttccggga atgagtctga tctcaagact ggcccagtcc
     2701 gggcgttgat tggcgctgag gagcatatcg catctcatca taatgtcgta tctcctgggg
     2761 tgttatacaa gatatcgttg ttggtgacct gggagaggaa ttgagttcta ttaaaccgtc
     2821 aactatgccg gatacatact ggattacact gcaggcacgc cttacgagag aacgtgccgc
     2881 agtgacgggt taattatctg aaagaatttg tgaggctgta tcggttactc attgatttga
     2941 tagttttact ctcgggagag taatagatat ttaatctatt aacggaaacc agccagttcc
     3001 tttcgatgcc tgaatttgat tccatagttt atccggaatg gttaattcat gaataagttg
     3061 tgttctcgtt catgagaagc ataacgtaaa gggaaaagct cgattagacg gcaggatttg
     3121 tcaggggtta tgaacgaaat tcataaatct gtttgagtgt tgcgatgggt agtgcaagtt
     3181 cgaaatctcc gcaatttaca gtccgatgaa ggaaaatgaa tatccataaa aaatatatcg
     3241 atttattctg gcgtatatac ctacttcgac gtatttccaa tagttgttaa ttaaaggcag
     3301 gccattatta ttcactctga ataatgaatt attcactgtc cgcagagtaa aaaatataac
     3361 ttaggtgtct atttaatgac ttgcacaaaa agctaaattt tcacccataa ataaaaatat
     3421 aatcccgcgc ccaaccacct gatgagtggc tacagacact ggatatatta ggtggcggtg
     3481 cactttctta cataaaggta tttccttttc tgcggaaaag gaaatcggga aatccccggt
     3541 ttttctgaca agcagacgcc attatttgtg tctgcctatg ttcgttaatt cgttcatcag
     3601 gaaattatct caatgtcgcg ttataaaaca ggtcataaac aaccacgatt tcgttattca
     3661 gttctggccc gctgcgtggc gtgggcaaat atctctgttc aggttctttt tccactcgct
     3721 gtcaccttta ccccagtaat ggcggcacgt gcgcagcatg cggttcagcc acggttgagc
     3781 atgggaaata ctacggtaac tgctgataat aacgtggaga aaaatgtcgc gtcgtttgcc
     3841 gcaaatgccg ggacattttt aagcagtcag ccagatagcg atgcgacacg taactttatt
     3901 accggaatgg ccacagctaa agctaaccag gaaatacagg agtggctcgg gaaatatggt
     3961 actgcgcgcg tcaaactgaa tgtcgataaa gatttctcgc tgaaggattc ttcgctggaa
     4021 atgctttatc cgatttatga tacgccaaca aatatgttgt tcactcaggg agcaatacat
     4081 cgtaccgacg atcgtactca gtcaaatatt ggttttggct ggcgtcattt ttcaggaaat
     4141 gactggatgg cgggggtgaa cacctttatc gaccatgatt tatcccgtag tcatacccgc
     4201 attggtgttg gtgcggaata ctggcgcgat tatctgaaac tgagcgccaa tggttatatt
     4261 cgggcttctg gctggaaaaa atcgccggat attgaggatt atcaggaacg cccggcgaat
     4321 ggttgggata tccgcgcaga gggctattta cctgcctggc cgcagcttgg cgcaagcctg
     4381 atgtatgaac agtattatgg cgatgaagtc gggctgtttg gtaaagataa gcgccagaaa
     4441 gacccgcatg ctatttctgc cgaggtgacc tatacgccag tgcctcttct gacactgagc
     4501 gccgggcata agcagggcaa gagcggtgag aatgacactc gctttggcct ggaagttaat
     4561 tatcggattg gcgaacctct ggagaaacaa ctcgatacag acagcattcg cgagcgtcga
     4621 atgctggcag gcagccgcta tgacctggtt gagcgtaata acaacatcgt tcttgagtac
     4681 cgcaaatctg aagtgatccg tattgctctg cctgagcgta ttgaaggtaa gggcggtcag
     4741 acactttccc tggggcttgt ggtcagcaaa gcaactcacg gactgaaaaa tgtgcagtgg
     4801 gaagcgccgt cattactggc tgaaggtggc aaaattaccg gtcagggtag tcagtggcaa
     4861 gtaacgctcc cggcttatcg tccaggcaaa gacaattatt atgcgatttc tgcggttgcc
     4921 tacgataaca aaggcaatgc ctcaaaacgc gtgcagacag aggtggtcat taccggagca
     4981 ggtatgagtg ccgatcgcac ggcgttaacg cttgacggtc agagccgtat tcaaatgctt
     5041 gctaacggta atgagcaaag accgctggtg ctgtctctgc gcgacgccga gggccagcca
     5101 gtcacgggca tgaaagatca gatcaagact gaactaactt tcaaaccggc tggaaatatt
     5161 gtgactcgta ccctgaaggc cactaaatca caggcaaagc caacactggc tgagttcacc
     5221 gaaactgaag caggggtgta tcagtctgtc tttactaccg gaacgcagtc aggtgaggca
     5281 acgattactg ttagtgttga tggcatgagc aaaaccgtca ctgcagaact gcgggccacg
     5341 atgatggatg tggcaaactc caccctgagc gctaacgagc cgtcaggtga cgtggttgct
     5401 gatggtcagc aagcctacac gctgacgctg actgcggtgg atactgatgg taacccggtg
     5461 acgggagagg ccagccgctt gcgatttgtt ccgcaagaca ctaatggtgt caccattggt
     5521 acaatttcgg agataaaacc aggcgtttac agcgctacgg tttcttcgac ccgtgccgga
     5581 aacgttgttg tgcgtgcttt cagcgagcag tatcagctgg gcacattaca acaaacgctg
     5641 aagtttgttg ccgggccgct tgatgcagca cattcgtcca tcaccctgaa tcctgataaa
     5701 ccggtggttg ggggtacagt tacggcaatc tggacggtaa aagatgccta tgacaaccct
     5761 gtgaccagcc tcacgccgga agcgccgtca ttagcgggtg ccgctgctgt aggttctacg
     5821 gcatcgggct ggacaaataa tggtgatggg acgtggactg cgcagattac tctcggctct
     5881 acggcgggtg aattagaagt tatgccgaag ctaaatggac aggatgcggc agcaaatgcg
     5941 gcaaaagtaa ccgtggtggc tgatgcgtta tcttcaaacc agtcgaaagt ctctgtcgca
     6001 gaagatcacg taaaagccgg cgaaagtaca accgtgacgc tggtggcgaa agatgcgcat
     6061 ggcaacgcta tcagtggtct ttcgttgtcg gcaagtttga cggggaccgc ctctgaaggg
     6121 gcgaccgttt ccagttggac cgaaaaaggt gacggttcct atgttgctac gttgactaca
     6181 ggcggaaaga cgggcgagct tcgtgtcatg ccgctcttca acggccagcc agcagccacc
     6241 gaagccgcgc agttgacggt catcgccgga gagatgtcat cagcgaactc tacgcttgtt
     6301 gcggacaata aggctccgac cgtcaaaacg acgacggaac tcaccttcac cgtgaaggat
     6361 gcgtacggga acccggtcac cgggctgaag ccagatgcac cagtgtttag cggtgccgcc
     6421 agcacgggga gtgagcgtcc ttcagcagga agctggacag agaaaggtaa tggggtctac
     6481 gtgtcgacct taacgctggg atctgccgcg ggtcagttgt ctgtgatgcc gcgagtgaac
     6541 ggccaaaatg ccgttgctca gccactggtg ctgaacgttg caggtgacgc atctaaggct
     6601 gagattggtg atatgacagt gaaggttaat aaccaactgg ctaatggaca gtctgctaac
     6661 cagataaccc tgaccgtcgt ggacagctat ggtaacccgt tgcaggggca agaagttacg
     6721 ctgactttac cgcagggtgt gaccagcaag acggggaata cagtaacaac caatgcggca
     6781 gggaaagtgg acattgagct tatgtcaacg gttgcgggag aacacaatat ttccgcttcg
     6841 gtgaatggtg ctcagaagac ggtcacggtg aaatttaacg cggatgccag caccggtcag
     6901 gcaaacctgc aggtagacac cgccgttcaa aaagtggcaa acggcaaaga cgcctttacg
     6961 ctgacggcga ccgttgagga taaaaatggt aaccctgttc cagggagcct ggtgaccttt
     7021 aatctgcccc ggggtgtcaa gccgcttaca ggcgataatg tctgggtgaa agccaacgat
     7081 gaggggaaag cagagttgca ggtggtttca gtgactgccg gaacctatga gatcacggcg
     7141 tcggcaggaa atgaccagcc ttcgaatgcg cagtctgtaa cgtttgtggc ggataagact
     7201 acggcaacta tctccaatat tgaggtgatt ggcaactatg cgctggcgga cggcaaagcc
     7261 aaacaaacgt ataaagttac ggtgactgat gccaataaca atttggtgaa agatagcgaa
     7321 gtgacgctga ctgccagccc ggcttcgtta aacctggaac cgaatggcac tgcgacaacg
     7381 aatgagcaag ggcaggctat tttcaccgct accactattg ttgcggcgac atacacactc
     7441 aaggcgcaag tgagtcagac caacggtcag gtatcaacga aaactgccga atctaaattc
     7501 gttgcggatg ataaaaacgc ggtactcacc gcatcatctg atatgcaatc tctggtggcg
     7561 gatgggaaat cgactgcgaa gctggcggtg accctgatgt cggcgaataa ccccgttggc
     7621 gggaatatgt gggtcgacat tcagacgccg gaaggggtga cggagaagga ttatcagttc
     7681 ctgccgtcga aaaatgacca tttcgtgagc gggaaaatca tacgtacatt tagtaccagc
     7741 aagccaggcg tctatacatt cacatttaac gccctgacct atggcgggta cgaaatgaag
     7801 ccagtgacgg tgaccattac cgcggtggat gccgatacgg caaagggcga ggaggcgatg
     7861 aaataatttt taagaatatg aaaatagcat tctaataaac aataatggcg ttgacatatt
     7921 caacgccatt atttattgaa ttgcctgaca gtagtctcgt tataagttca tttagatata
     7981 gcgacagcta aaccggacga acggggataa aaatttcgca aacaacatca ttgctatcct
     8041 tttctacgaa atcttcattg taataaaatc gctcaatatc actgccgttc actctggcta
     8101 accccctggc aggtaattcc atcagataga ggtttcgcga ccattcagga tagcgctccc
     8161 actcaccttc atagcggaaa gatgcataca gtcctcggtt tattgtccac tctttcgcca
     8221 tctcagaatc ggtttgttta cataccgtac cggcaaaagt ttcaactgtg agcaaatcga
     8281 ccgattttgt tgacggttca aacgtggaga cgaaatagat atccgtctta aaattattca
     8341 tacatctggc taaatgatga cgtcgcagtt tgacttcttc atccggtgat ttattcgaca
     8401 cttcaatgtc atatttaaat gagttgccaa aaataggtgt ctcaggaaaa ttgactaatc
     8461 tacactccgt cttttgctgt tgacgtatta aaaaagaagg gaagatattt gcgagatccc
     8521 aataatcacg gtggcggtat tcacggggag agcagccgaa taacttttta aattcgcggc
     8581 tgaatgactg ctgtgaatca aaatgcaaag agagtgcaat atcaagcata gatttcgcgg
     8641 tgagccggac aagaatagcg gcacggcaaa gccttcgttt gcgaatgtat tctcccaaag
     8701 gcacatgcat gaaatttcgg aacagaagct ggatgttgcg tctgctgtag ccagatttct
     8761 gtgcgatatc ttcgattgaa atagggtgct caagattgca ctcaatccac tccaggagct
     8821 gctgtagaat cttctgcctg atcatcacat tctccattga ttcttcgtca tatcgcacat
     8881 tttcgttatg ccctgggccc taagtgtatc aaaaacagca agtccgaagg caaaagcact
     8941 ggtagcgtct gcttcgttgt ccccgaaaat agagaggttt cagtcctaca ttattaatga
     9001 attttttgca taagtgatat caaaatccac atactaattt gaggttacgt tttaacgtag
     9061 actcattgtt catgcctaat ggagggactg acagtggaat tttccgtatt aagta
//
LOCUS       DAZGAN020000163         8767 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZGAN020000163 DAZGAN020000000
VERSION     DAZGAN020000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.163"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(28..1569)
                     /gene="tyrR"
                     /locus_tag="ACHKYO_RS22780"
                     /old_locus_tag="pgaptmp_004556"
     CDS             complement(28..1569)
                     /gene="tyrR"
                     /locus_tag="ACHKYO_RS22780"
                     /old_locus_tag="pgaptmp_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HGU4431266.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(1717..2778)
                     /locus_tag="ACHKYO_RS22785"
                     /old_locus_tag="pgaptmp_004557"
     CDS             complement(1717..2778)
                     /locus_tag="ACHKYO_RS22785"
                     /old_locus_tag="pgaptmp_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HGU4431267.1"
                     /translation="MTEPLKLRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(2775..4172)
                     /locus_tag="ACHKYO_RS22790"
                     /old_locus_tag="pgaptmp_004558"
     CDS             complement(2775..4172)
                     /locus_tag="ACHKYO_RS22790"
                     /old_locus_tag="pgaptmp_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HGU4431268.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTNDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            4328..5326
                     /locus_tag="ACHKYO_RS22795"
                     /old_locus_tag="pgaptmp_004559"
     CDS             4328..5326
                     /locus_tag="ACHKYO_RS22795"
                     /old_locus_tag="pgaptmp_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4431269.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(5437..6342)
                     /gene="ompG"
                     /locus_tag="ACHKYO_RS22800"
                     /old_locus_tag="pgaptmp_004560"
     CDS             complement(5437..6342)
                     /gene="ompG"
                     /locus_tag="ACHKYO_RS22800"
                     /old_locus_tag="pgaptmp_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HGU4431270.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(6387..7469)
                     /locus_tag="ACHKYO_RS22805"
                     /old_locus_tag="pgaptmp_004561"
     CDS             complement(6387..7469)
                     /locus_tag="ACHKYO_RS22805"
                     /old_locus_tag="pgaptmp_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4431271.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(7483..8142)
                     /gene="pgmB"
                     /locus_tag="ACHKYO_RS22810"
                     /old_locus_tag="pgaptmp_004562"
     CDS             complement(7483..8142)
                     /gene="pgmB"
                     /locus_tag="ACHKYO_RS22810"
                     /old_locus_tag="pgaptmp_004562"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGU4431272.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(8139..>8767)
                     /gene="ycjT"
                     /locus_tag="ACHKYO_RS22815"
                     /old_locus_tag="pgaptmp_004563"
     CDS             complement(8139..>8767)
                     /gene="ycjT"
                     /locus_tag="ACHKYO_RS22815"
                     /old_locus_tag="pgaptmp_004563"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HGU4431273.1"
                     /translation="YKAAAGKQTILLDYSRAEVNEMQILKQADVVMLNYMLPEQFSAA
                     SCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDLGADPHSCDD
                     GIHAAATGAIWLGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDA
                     QRIAIRTSAPVSLRLNGQLISVAEESVFCLGDFILPFNGTATTHQEDE"
BASE COUNT         2141 a         2437 c         2100 g         2089 t
ORIGIN      
        1 gtgttgcacc atcaggcata ttcgcgctta ctcttcgttc ttcttctgac tcagaccata
       61 ttcccgcaac ttattggcaa tcgcggtatg tgaaacgccg agacgttttg ccagtttgcg
      121 cgtgctggga taattgcgat aaagctgggt taataccgag cgttcaaaac ggctggtgat
      181 ttcgtccagc gaaccttcca tcgcatcttc gcccacggct accgttgcgg cgtcataatc
      241 cggcaacaaa atatcctgtg gacgcagctc ataaccgtcc agttgtgtca gtgcgcgata
      301 gatagcgttc tttaactgcc gcacatttcc cggccacgca taacgcgtaa gtacagtatt
      361 caggtcagcg gccagtttcg gacgcggcac gccctgctcg tcggcaaagc gggcgacgaa
      421 cagctcagtt aacggcatga tgtcctgcgg acagtcacgt agcggcggca gattgagcgt
      481 caacacgttc agacgataat agagatcttc acggaacatg cctttttgca ccagttcgac
      541 cagattcttc tgcgtagcgc aaatcacccg cacatcgaca tgcacctcat ggtcttcgcc
      601 aacccgacgg aaagtgccat cattaaggaa acgcagtaat ttcgcctgca tccgtggtga
      661 catttcccct atttcatcca acagcaccga accaccgttc gcctgctcaa agaatccttt
      721 cttcccttcc ggagcatgac caaacagttc actctcgacc gcatcttccg gtatagacgc
      781 acagttcagc gccaggtaag gtttgcccgc tctggggctt gcctgatggc aggcgtaggc
      841 aaagagatct ttacctgtac ctgtgtcacc cgtaatcagc agcggcgcgc ttagcatcgc
      901 cagtttctgc gcctgttcga caacatgctt cattttcggg ctgacggcga caatttgact
      961 gaaggcgctg acgtcctggg cggcgacatt ttgcaactgg cggcccatac gaatcgttga
     1021 tcgcaacatc accaccgcac cggtcaggac gtgttgatca ttttcatcct gaagataaac
     1081 aggcgtaatc tccatcagga aattctgccc attaataacg acatgctcgt tatgggaatc
     1141 ttgcggttcg ctttccagcc aacgtaaaaa attaaagccg ttaatcaatt gtgcggcggt
     1201 atggttgcgc aggcgatcca atttttgcgc aaaaagctga cagctcgccg ggttcgccat
     1261 atccactttg cttttcatat cgacagagag cacaggttcg ggcaacgcct ccagtaacgc
     1321 gctcaacgcc agatgctcac gttcggaagg catccacggg acagtacgca catcggtaac
     1381 acctgcaata cggcgtattt cggccatcag actgctgaaa ctctcaaact ccagttcagc
     1441 aaaattgagg tagattcgcc caatgggatc aatctcaata ccgcgtaaat caatgcctct
     1501 tagcacgagt agatcgagta attcgcgggt cagaccgagt cggtcttcac aaaagacttc
     1561 cagacgcatg ggaaccttca cctgaaaaaa gaacaattaa tatgatgata tgacactatt
     1621 tggatagcag gaaggtttct gtcaacaatc attgacacaa agacggaaaa acgggcactt
     1681 cggcgtaaag atatccggtc agcacccgat attgcattat ttttcgctgg gcgttttgcc
     1741 tttttgcagc gtttctttca cctgaccgat aagctgacga cggaaatccc cgaggcgagg
     1801 tttgtcatcg tcaatccacg gcagcgggcg gcaaagctcc atagctttaa tcccgagtcg
     1861 tgccgtcaga agtccagcac caattccctg agctgcgcgg gtagacaaac gagcagcgag
     1921 atcttgcgac atccagtcca tccccacttc gcgcaccagt tcgctggctc cggcaaaagc
     1981 gatattcagc aataccagct taaacagacg caaacggctg taatacccca gttcaatgcc
     2041 atacagcgtg gcgatgcgat taattaaacg cagattgcgc caggcgataa acgccatatc
     2101 gaccaacgcc agcgggctga ccgcaatcat caacgttgat tccgccgccg aacggctgat
     2161 ttcgcgccga gcctgggcat ctaaaactgg ctggaccaaa tgcgcataca aactgaccac
     2221 ttcacggtcg ttttgcgttt catggattga ggcataccag cgttgcagcg ctggatgaga
     2281 ctgatcaata cccgcctgct gcgccagttt ttcgcaaaat gcgcggcctt tgcccgtgcc
     2341 gtggctgtgc aacaaatcgc gcgcttcgtc gcgttcatgg gcgcgctgtc gcaagcgcca
     2401 taagcgccgc cactctgtta ccacagaacc tacgccagcg ccgataatca atgccccagc
     2461 ggcacatcca cccagcgcca cccagtcctg agtttgccag gcattcattg tccactgtac
     2521 accctgcccg acaacgcttg cgccaaacag agccagcccg cccatcacca ttttgcgcca
     2581 caggctgcgt ttcggacgta acgctgcatc cattaccgct tcaacttgcc cctcttcttc
     2641 aggcgcttca tcgagcgtgg ccggggcaaa attttgcgcc tgattttcgt caaaggtctg
     2701 ctgcgccctg aattttggat tctgatcgac ctccagcgga ccgtcgaaat caatacgtag
     2761 ttttaacggt tcggtcatcg caatttatct cctattaaaa attcaagcgc ggcatcaaga
     2821 cgaatatgcg gcagcggttt gtcgacatcc atcacctgcg ggcgaaacgc ttcaaactgg
     2881 aacccttgct tgtcccagaa cgcctgaccg ggcaaacgcg caggaacttc accgggataa
     2941 acggtgagtg gcgcaccatc gctgagccgg ttaccgcgca atgccggaat tttctcgccg
     3001 ttgacgtcaa taatgccgct ggtggtcgcc tgcaccgagg ccagccccag gcaatccata
     3061 ctaatccctt caaacgccgc attttgccag gcatcctgaa tcagttgttg cagtaatgaa
     3121 accatattgg cgtgctgatc gttggtcaca tggtccgctt tagtggcagc aaacaatagc
     3181 ttatcgataa ccggcgaaaa caaacgccgg aacaaggtac gctgcccgta gtggaaactt
     3241 tgcatcagct gcgtcagtgc cagacgcata tcattaaatg cctgtggccc actgttgaga
     3301 ggttgcaggc aatcaaccag cacaatctgg cggtcaaagc gcagaaaatg attcttatag
     3361 aaccccttca ccaccttctc gcagtaataa ttaaaccgct cgcgcagcat tccggcattg
     3421 gtgtgcttat cggcctgcgc cagtttggac tcgccccagg catcgacatc cggccacggg
     3481 aagaattgca gcgcgggcgc acctgccata tctcccggca agacaaagcg cccaggctga
     3541 ataaagtgca gcccctgctg tttacagtgg tggagataat cggtccacgc ggcggcaatg
     3601 tctgccagcc ggttttcgtc ggcaggcgct agcgggtcca gcccttcgca catcattcgc
     3661 catttgaccg accattcgcc acgctgacca ttgagtaagc ccgtcatctg gcgtgaccag
     3721 cttaaatagt cctgcgccag catcggcagg tcgagcaacc attcgccggg ataatccaca
     3781 atctccagat acagcgtgga ggtgtccttg aagtggcgta gcagcgaatc gttcgattta
     3841 aaacgtagcg ccaggcggat ttcactgacg ccgcgcgttg gcgtcggcca ggcgggagga
     3901 tcgccatata actgcgccag tccttcatca tatgtgaagc gcggaatgcc aaagtcacgc
     3961 tggggaatgc gtttcacgcc cagcaggcgc tcttcacgca ccgcacttaa cagcggcaaa
     4021 cgtgctccgg cgtgaatatt aagcaactga ttaaccatcg cggtgataaa cgctgttttg
     4081 ccgctgcggc taagtccggt tacagcgagg cgcagatgtc tgtcgacacc ccgattcacc
     4141 agcgcattga gttcattttt aagtcgcttc attgctgtcc tgtgctactc tgaagaccag
     4201 aaaaaaaatc attcgtaagg atatgaatgt gggggccagg ccattgatta tcaactgccc
     4261 gtgttgtcat cgaccggcga actccataag atgtgcagca ttcacgttcg gacggagttc
     4321 ctgacctatg tcccctacta tttatgatat tgccagggtt gcaggcgtat caaaatccac
     4381 cgtatcacgc gtgctgaata agcaaactaa tatctccccg gaagcgcgcg aaaaagtgtt
     4441 acgggccatt gaagaattac agtatcagcc aaacaagctg gcccgcgcgc tgacctcttc
     4501 gggttttgat gccattatgg tgatttctac ccgttcgacc aaaactacgg cgggtaatcc
     4561 gtttttctct gaagttctac atgccatcac cgccaaagcg gaagaagaag gtttcgacgt
     4621 gatattgcag acgtcgcaca acctggcaga agacttacaa aaatgtgaaa gcaaaattaa
     4681 gcagaaaatg attaaaggca ttattatgct aagttcgcca gcggatgagt cattttttgc
     4741 ccaacttgat aaatacgata ttcctgtcgt ggtgattggc aaagttgaag gtcaatatgc
     4801 ccatgtttat tctgtcgata ccgataattt tggcgacagc attgcgttga ccgatgcatt
     4861 aattgaaagt gggcatcaaa atattgcctg cctgcatgct ccgcttgatg tccatgtttc
     4921 agtggatcgg gtaaatggtt ataagcagag cctggctgcg cataatattg cagtgcgtga
     4981 tgaatggatt gttgacggcg gttataccca tgaaacagca ctgcaagccg cgcgggaatt
     5041 attaagccag tcaccgttgc ctgaggcagt gtttgctact gacagcctga aattaatgag
     5101 tatttatcgt gcggcagcag agaaaaatat tgctattccg cagcagttag cggtggtggg
     5161 ttatagtaat gaaacgctgt cgtttatttt aacgcctgcg ccaggcggta ttgatatccc
     5221 aacacaggag ttaggtcaac aaagctgtga gttattattc cgcttaattt ctggaaaacc
     5281 gtcaccacag aatattaccg ttgccacgca tatgacgctg aaataaatac cagctacctt
     5341 gcctgaatgc cggatgcgat gctagcgcat cttatccggc ctacatttat agttcggata
     5401 aagtactcgt gccgcatccg gcaatttagc ccattatcag aacgagtaat ttacgccgac
     5461 acctgcataa tggaatttat cgctgtcgcc ttcgtcgtga tcctgccact caaacgcgta
     5521 ttccagcgaa acggaaagcc cgttctggaa atcataaccg taaaataaac ctacacggtt
     5581 aaaatcatgg ccttcacgtt caatatcatc ctgccagtcc cagttactcc agcgatccag
     5641 cccaatgcgc gtatacggcg tcaccgagtg gttgccgagc gtcagcggca aataggcgcg
     5701 aatttcttgc gtagaaaact caccgttatt gcggctgtcg tccatattaa agccgcgctc
     5761 gagataatag ttcactcgca aggcaaccgt ttcgttgaag gtatattgca ggcctgtttc
     5821 cgtttcgact cgggtatcag cgtaaccggt cgtgttcaga tcgttggcaa atttatacat
     5881 cgacaaccaa ccgttgaaac gtaaatcgtc agtcagtttc acatcccagt ctggcgcgat
     5941 tttccagcgc tgcatattcg ccgtgtcttt acccggttca tcaacgtagt gataaccata
     6001 attacggaaa ccgccggtca ggccgaaact gaaatcatcg ttttcgagga actgataatg
     6061 cacctccagc tccgggcgat caaaccacgt tccacgttta cccgcgctat aatctaccgg
     6121 cccttcctga taataggcca gagcaattct ccacggcccg ttggcggcat taaaatagac
     6181 tgaaggctcc gccagcccat ccatatcttc gccataaccc tcgacgtttt ctatttcgta
     6241 catcgcgccg atattaaagt gccagtcgtt cctttcctcg gcctgtgcgc aggccattcc
     6301 cgcacacatc accagtgcgg tacagggtaa taactttttc attattgttc cttgtattat
     6361 tctgggggaa tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag
     6421 aaatgacatt tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt
     6481 aacgcaccag cacggaccac taactcgtgc cccccaaccg tggtgtagag cataaattcc
     6541 gcaccggtta attctgccac gctaattttg gcggaaatgt tattttcctc ttgcgcgtcc
     6601 ggatgaatat cttccggacg tattcccatc acgatgggct tatgcaaact tccctgtgtt
     6661 ttcagaaccg ctaatttctc ttcgggaatg gttaatttaa gcgtttccgt aacgaattta
     6721 tcgccatcga tcgtgccacg aataaaattc atcgccggtg atccaataaa tccggcaaca
     6781 aacatattcg ctggttggtt ataaaccgtt ttcggcgcac caacttgctg aacaatccca
     6841 tccttcataa tcacaatccg cgtcgccatg gtcatcgctt cggtctgatc gtgggtcacg
     6901 tagatcatgg tggtattcag cttctggtgt agcttgctga tctctgcgcg catttgcacg
     6961 cgcagcttgg catcaaggtt agagagcggt tcatccatta aaaacacgcc cgcttcgcgc
     7021 acaatcgccc gcccgagcgc cacacgctgg cgttgcccgc cggaaagcgc gcccggcttg
     7081 cgtttcaggt actcacgcag gccgagaatt tgcgccgccc agttcacccg ctcatcaatc
     7141 acctctttgg cgattttttg catcttcagg ccaaacgcca tgttgtcgta aaccgtcata
     7201 tgcggataca acgcgtagtt ctggaacacc atcgctatat tgcgtgcttt ggctggaacg
     7261 tcattcattc gtttgccgtc gatcaacaga tcgccgccgc tgatctcctc aagcccggca
     7321 atcatgcgca gggtggtcga cttaccgcag cccgacgggc cgacaaacac gatgaactct
     7381 ttatcggcaa tttccaggtt gaagtccttc accacatgca cctggttatc gtagattttt
     7441 tgaatatgtt gtaacgaaag ctgagccatg ttgattcctt tgctatacgt tttgccagaa
     7501 ggccgataac cgcgaccagg tcagagagtc cgttgaaggc aacagtaatt gcgccccggt
     7561 taagcccgcg ccgatcccca ccgagcgcat accgctggcg ttaatggcgt caatgcccgc
     7621 ctgcgcatct tcaatgccga tacatgcctg cggcggcacg cccagcccgg cacaggcggc
     7681 gagaaagatt tccgggtccg gtttcgagtt tttaagttgg gaagcatccg cgcagaaggt
     7741 gaaaaactcg cgcagctcca gcgccgctaa aatcgtcggc gcattcaggg agacagaagc
     7801 cagcccaacc gggatctgct gtgcacggag atctgccagc aaagagcgaa tgccgggtag
     7861 aacagcgctg accgtcaact cgcgcagtga gtggacatag agcagatttt tgcgatacgc
     7921 cagttgcgcc ctctcctgcg agttaaagtc gccctctttg cccccgtgtt gcagaatgcg
     7981 ccgcagagac tcatcgcggc tgatcccttt tagggattcg ttaaactgcg catcaatgct
     8041 gatgccaatt tcagcggcaa tctgctgcca cgcctggaaa tgcagatgcg cggtatcggt
     8101 gattacacca tccagatcga aaattacccc ttgcagtttc attcatcctc ctgatgtgtg
     8161 gtagcggtcc cattgaaggg caaaataaaa tcacccaaac agaaaacaga ttcttcagcc
     8221 acggatataa gctgaccgtt caaacgcagt gaaacgggcg cagaagttcg aatcgcaata
     8281 cgctgcgcgt caagagtgac ctgtaattcg cagccctgcc agaacagagg gaaagacaac
     8341 tgttgccact gctcaggtaa cgccggattg agatgcaatt caccgtcacg cacgctcacc
     8401 ccggcaaaac cctgaatcgc ccccagccag atagcgccag ttgcggcagc atggatacca
     8461 tcatcacaac tatgcggatc agcaccaaga tcgatttcag tcccctcgcg ccagaactga
     8521 taactttggg tcagcaggcc acagcgtgcg gcaacaatgc cgtggattgc tttacttaat
     8581 gacgagtcgt gaatagtgcg cggttcataa aattgcagat tggcaagaca cgatgccgct
     8641 gagaactgct ccggcagcat gtaattgagc atcaccacat cagcttgttt gaggatttgc
     8701 atctcgttca cttctgcgcg tgaataatcc agcagaatgg tttgcttccc cgccgccgct
     8761 ttgtatt
//
LOCUS       DAZGAN020000164         8750 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGAN020000164 DAZGAN020000000
VERSION     DAZGAN020000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.164"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(365..628)
                     /gene="rpsT"
                     /locus_tag="ACHKYO_RS22820"
                     /old_locus_tag="pgaptmp_004564"
     CDS             complement(365..628)
                     /gene="rpsT"
                     /locus_tag="ACHKYO_RS22820"
                     /old_locus_tag="pgaptmp_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGU4431274.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            731..949
                     /locus_tag="ACHKYO_RS22825"
                     /old_locus_tag="pgaptmp_004565"
     CDS             731..949
                     /locus_tag="ACHKYO_RS22825"
                     /old_locus_tag="pgaptmp_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HGU4431275.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            957..1898
                     /gene="ribF"
                     /locus_tag="ACHKYO_RS22830"
                     /old_locus_tag="pgaptmp_004566"
     CDS             957..1898
                     /gene="ribF"
                     /locus_tag="ACHKYO_RS22830"
                     /old_locus_tag="pgaptmp_004566"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGU4431276.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1941..4757
                     /gene="ileS"
                     /locus_tag="ACHKYO_RS22835"
                     /old_locus_tag="pgaptmp_004567"
     CDS             1941..4757
                     /gene="ileS"
                     /locus_tag="ACHKYO_RS22835"
                     /old_locus_tag="pgaptmp_004567"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGU4431277.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4757..5251
                     /gene="lspA"
                     /locus_tag="ACHKYO_RS22840"
                     /old_locus_tag="pgaptmp_004568"
     CDS             4757..5251
                     /gene="lspA"
                     /locus_tag="ACHKYO_RS22840"
                     /old_locus_tag="pgaptmp_004568"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGU4431278.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5339..5788
                     /gene="fkpB"
                     /locus_tag="ACHKYO_RS22845"
                     /old_locus_tag="pgaptmp_004569"
     CDS             5339..5788
                     /gene="fkpB"
                     /locus_tag="ACHKYO_RS22845"
                     /old_locus_tag="pgaptmp_004569"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4431279.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5790..6740
                     /gene="ispH"
                     /locus_tag="ACHKYO_RS22850"
                     /old_locus_tag="pgaptmp_004570"
                     /gene_synonym="lytB"
     CDS             5790..6740
                     /gene="ispH"
                     /locus_tag="ACHKYO_RS22850"
                     /old_locus_tag="pgaptmp_004570"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HGU4431280.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6806..7720
                     /gene="rihC"
                     /locus_tag="ACHKYO_RS22855"
                     /old_locus_tag="pgaptmp_004571"
     CDS             6806..7720
                     /gene="rihC"
                     /locus_tag="ACHKYO_RS22855"
                     /old_locus_tag="pgaptmp_004571"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HGU4431281.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     ALLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7887..8708
                     /gene="dapB"
                     /locus_tag="ACHKYO_RS22860"
                     /old_locus_tag="pgaptmp_004572"
     CDS             7887..8708
                     /gene="dapB"
                     /locus_tag="ACHKYO_RS22860"
                     /old_locus_tag="pgaptmp_004572"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGU4431282.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         2001 a         2167 c         2484 g         2098 t
ORIGIN      
        1 gattgttgtt aattatttac aagatctaat aaggagatat ttatgaatgg ttaaagtgta
       61 taggaattta attcaaaaat agagtggtct taagtttaat acctcaatgt gtatcacaat
      121 atccacattc tttgtagggg agtctggaga ttgagtagat attcttgttc agaatgtatc
      181 agccgatggt tctacgattc ttaagccacg aagagttcag aaagtgtaac ggcatgtctg
      241 caaaagccag catcttttga ctatctggca accggtagtg tgttctctca cgcatcataa
      301 aagcagcagg cataaaaaaa cccgcttgcg cgggcttttt cacaaagctt cagcaaattg
      361 gcgattaagc cagtttgttg atctgtgcag tcaggttagc cttatgacgt gcagctttgt
      421 ttttgtggat cagaccttta gcagcctgac ggtccacgat cggttgcatt tcgttaaatg
      481 ctttctgtgc agcagctttg tcgccagctt cgatagctgc gtatactttc ttgatgaaag
      541 tacgcatcat agagcgacgg cttgcgttgt gcttacgagc cttttcagac tgaatggcgc
      601 gcttcttagc tgatttgata ttagccaagg tccaactccc aaatgtgttc tatatggaca
      661 attcaaaggc cgaggaatat gcccttttag ccttcttttg tcaatggatt tgtgcaaata
      721 agcgccgtta atgtgccggc actcgttacg tagtgatggc gcaggattct accagcttgc
      781 ggggtgtgaa tacagctttt ccgcgataaa aattgcagca ggcggtcact ttcttcccgt
      841 gatttgcgcc atggcaatga aaagccactt ctttctgatt tcggtactca atcgccggtt
      901 aaccttgacc gctgtacaag gtatactcgg acgattttca ctgttttgag ccagacatga
      961 agctgatacg cggcatacat aatctcagcc aggccccgca agaagggtgt gtgctgacta
     1021 ttggtaattt cgacggcgtg catcgcggtc atcgcgcgct gttacagggc ttgcaggaag
     1081 aagggcgcaa gcgcaactta ccggtgatgg tgatgctttt tgaacctcaa ccactggaac
     1141 tgtttgctac cgataaagcc ccggcacggc tgacccggct gcgggaaaaa ctgcgttacc
     1201 ttgcagagtg tggcgttgat tacgtgctgt gcgtgcgttt cgacaggcgt ttcgcggcgt
     1261 taaccgcgca aaatttcatc agcgatcttc tggtgaagca tttgcgcgta aaatttcttg
     1321 ccgtaggtga tgatttccgc tttggcgctg gtcgtgaagg cgatttcttg ttattacaga
     1381 aagctggcat ggaatacggc ttcgatatca ccagtacgca aactttttgc gaaggtggcg
     1441 tgcgtatcag cagcaccgct gtgcgtcagg cgctggcgga tgacaatctg gctctggcag
     1501 aaagtttact ggggcacccg tttgccatct ccgggcgtgt agtccacggt gatgaattag
     1561 ggcgcactat aggtttcccg acggcgaatg taccgctacg ccgtcaggtt tccccggtga
     1621 aaggggttta tgcggtagaa gtgctgggcc tcggtgaaaa gccgttaccc ggcgtggcaa
     1681 acattggaac acgcccaacg gttgccggta ttcgccagca gctggaagtg catttgttag
     1741 atgttgcaat ggacctttac ggtcgccata tacaagtagt gctgcgtaaa aaaatacgca
     1801 atgagcagcg atttgcgtcg ctggacgaac tgaaagcgca gattgcgcgt gatgaattaa
     1861 ccgcccgcga attttttggg ctaacaaaac cggcttaagc ctgttatgta atcaaaccga
     1921 aacacggaac cgagaatctg atgagtgact ataaatcaac cctgaatttg ccggaaacag
     1981 ggttcccgat gcgtggcgat ctcgccaagc gcgaaccggg aatgctggcg cgttggactg
     2041 atgatgatct gtacggcatc atccgtgcgg ctaaaaaagg caaaaaaacc ttcattctgc
     2101 atgatggccc tccttatgcg aatggcagca ttcatattgg tcactcggtt aacaagattc
     2161 tgaaagacat tatcgtgaag tccaaagggc tttccggtta tgactcgccg tatgtgcctg
     2221 gctgggactg ccacggtctg ccgatcgagc tgaaagtaga gcaagaatac ggtaagccgg
     2281 gtgagaaatt caccgccgcc gagttccgcg ccaagtgccg cgaatacgcg gcgacccagg
     2341 ttgacggtca acgcaaagac tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt
     2401 acctgaccat ggacttcaaa actgaagcca acatcatccg cgcgctgggc aaaatcatcg
     2461 gcaacggtca cctgcacaaa ggcgcgaagc cagttcactg gtgcgttgac tgccgttctg
     2521 cgctggcgga agcggaagtt gagtattacg acaaaacttc tccgtccatc gacgttgctt
     2581 ttcaggcggt cgatcaggat gcactgaaag caaaatttgc cgtaagcaac gttaacggcc
     2641 caatctcgct ggtgatctgg accactacgc cgtggactct gcctgcgaac cgcgcaatct
     2701 ctattgcacc tgatttcgac tatgcgctgg tgcagatcga cggtcaggcc gtgattctgg
     2761 cgaaagatct ggttgaaagc gtaatgcagc gtatcggcgt gaccgattac accattctcg
     2821 gcacggtaaa aggtgcggag cttgagttgc tgcgctttac ccatccgttt atgggcttcg
     2881 acgttccggc aatcctcggc gatcacgtta ccctggatgc cggtaccggt gccgttcaca
     2941 ccgcgcctgg ccacggcccg gacgactatg tgatcggtca gaaatacggc ctggaaaccg
     3001 ctaacccggt tggcccggac ggcacttatc tgccgggcac ttatccgacg ctggatggcg
     3061 tgaacgtctt caaagcgaac gacatcgtcg ttgcgctgct gcaggaaaaa ggcgcgctgc
     3121 tgcacgttga gaaaatgcag cacagctatc cgtgctgctg gcgtcacaaa acgccgatca
     3181 tcttccgcgc gacgccgcag tggttcgtca gcatggatca gaaaggtctg cgtgcgcagt
     3241 cactgaaaga gatcaaaggc gtgcagtgga tcccggactg gggccaggcg cgtatcgagt
     3301 cgatggttgc taaccgtcct gactggtgta tctcccgtca gcgcacctgg ggcgtaccga
     3361 tgtcactgtt cgtgcacaaa gacacggaag agctgcatcc gcgtactctc gaactaatgg
     3421 aagaagtggc aaaacgcgtt gaagttgacg gcatccaggc gtggtgggat cttgatgcga
     3481 aagagatcct cggcgatgaa gctgatcagt acgtgaaagt gccggacaca ttggatgtat
     3541 ggtttgactc cggatctacc cactcttctg ttgttgacgt gcgtccggaa tttgccggtc
     3601 acgcagcgga catgtatctg gaaggttctg accagcaccg tggttggttc atgtcttccc
     3661 taatgatctc caccgcgatg aaaggcaaag cgccgtatcg tcaggtactg acccacggct
     3721 ttaccgtgga tggtcagggt cgcaagatgt ctaaatccat cggcaatacc gtttcgccgc
     3781 aggatgtgat gaacaaactg ggcgcggata ttctgcgtct gtgggtggca tcaaccgact
     3841 acaccggtga aatggccgtt tctgacgaga tcctgaaacg tgctgccgat agctatcgtc
     3901 gtatccgtaa caccgcgcgc ttcctgctgg caaacctgaa cggttttgat ccagcaaaag
     3961 atatggtgaa accggaagag atggtggtac tggatcgctg ggccgtaggt tgtgcgaaag
     4021 cggcacagga agacatcctc aaggcgtacg aagcatacga tttccacgaa gtggtacagc
     4081 gtctgatgcg cttctgctcc gttgagatgg gttccttcta cctcgacatc atcaaagacc
     4141 gtcagtacac cgccaaagcg gacagtgtgg cgcgtcgtag ctgccagact gcgctgtatc
     4201 acatcgcaga agcgctggtg cgctggatgg caccaatcct ctccttcacc gctgatgaag
     4261 tgtggggcta cctgccgggc gaacgtgaaa aatacgtctt caccggtgag tggtacgaag
     4321 gcctgtttgg cctggcagac agtgaagcga tgaacgatgc gttctgggac gagctgttga
     4381 aagtgcgtgg cgaagtgaac aaagtcattg agcaagcgcg tgccgacaag aaagtgggtg
     4441 gctcgctgga agcggcagta accttgtatg cagaaccgga actgtcggcg aaactgaccg
     4501 cgctgggcga tgaattacga tttgtcctgt tgacctccgg cgctaccgtt gcagactata
     4561 acgacgcacc tgctgatgct cagcagagcg aagtactcaa agggctgaaa gtcgcgttga
     4621 gtaaagccga aggtgagaag tgcccacgct gctggcacta cacccaggat gtcggcaagg
     4681 tggcggaaca cgcagaaatc tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa
     4741 aacgtaagtt tgcctgatga gtcaatcgat ctgttcaaca gggctacgct ggctgtggct
     4801 ggtggtagtc gtgctgatta tcgatctggg cagcaaatac ctgatcctcc agaactttgc
     4861 tctgggggat acggtcccgc tgttcccgtc gcttaatctg cattatgcgc gtaactatgg
     4921 cgcggcgttt agtttccttg ccgatagcgg cggctggcag cgttggttct ttgccggtat
     4981 tgcgattggt attagcgtga tcctggcagt gatgatgtat cgctcgaagg ccacgcagaa
     5041 gctaaacaat atcgcttatg cgctgattat tggcggcgcg ctgggcaacc tgttcgaccg
     5101 cctgtggcac ggcttcgttg tcgatatgat cgacttctac gtcggcgact ggcacttcgc
     5161 caccttcaac cttgccgata ctgccatttg tgtcggtgcg gcactgattg tgctggaagg
     5221 ttttttgcct tctaaagcga aaaaacaata ataaaccctg ccggataagg cctacagatt
     5281 gctgcgaaat cgtaggccgc atccggcaaa aatcctcaaa atataagagc aaacctgcat
     5341 gtctgaatct gtacagagca atagcgccgt cctggtgcac ttcacgctga aactcgatga
     5401 tggcaccacc gctgagtcta cccgcaacaa cggtaaaccg gcgctgttcc gcctgggcga
     5461 tgcttctctt tctgaagggc tggagcaaca cctgttgggg ctgaaagtgg gcgataaaac
     5521 caccttctcg ctggagcccg atgcggcgtt tggcgtgccg tcaccggacc tgattcagta
     5581 cttctcccgc cgtgaattta tggatgcagg cgagccagaa attggcgcaa tcatgctttt
     5641 taccgcaatg gatggcagtg agatgcctgg cgtgatccgc gaaattaacg gcgactccat
     5701 taccgttgat ttcaaccatc cgctggccgg gcagaccgtt cattttgata ttgaagtgct
     5761 ggaaatcgat ccggcactgg aggcgtaaca tgcagatcct gttggccaac ccgcgtggtt
     5821 tttgtgccgg ggtagaccgc gctatcagca ttgttgaaaa cgcgctggcc atttacggcg
     5881 caccgatata tgtccgtcac gaagtggtac ataaccgcta tgtggtcgat agcttgcgtg
     5941 agcgtggggc tatctttatt gagcagatta gcgaagtacc ggacggcgcg atcctgattt
     6001 tctccgcaca cggtgtttct caggcggtac gtaacgaagc aaaaagtcgc gatttgacgg
     6061 tatttgacgc gacctgtccg ctggtgacca aagtgcatat ggaagtcgcc cgcgccagtc
     6121 gccgtggcga agaatctatt ctcatcggtc acgccgggca cccggaagtg gaagggacaa
     6181 tgggccagta cagtaacccg gaagggggaa tgtatctggt cgaatcgccg gacgatgtgt
     6241 ggaaactgac ggtcaaaaac gaagagaagc tctcctttat gacccagacc acgctgtcgg
     6301 tggatgacac gtctgatgtg atcgacgcgc tgcgtaaacg cttcccgaaa attgtcggtc
     6361 cgcgcaaaga tgacatctgc tacgccacga ctaaccgtca ggaagcggta cgcgccctgg
     6421 cagaacaggc ggaagttgtg ttggtggtcg gttcgaaaaa ctcctccaac tccaaccgtc
     6481 tggcggagct ggcccagcgt atgggcaaac gcgcgttttt gattgacgat gcgaaagata
     6541 tccaggaaga gtgggtgaaa gaggttaaat gcgtcggcgt gactgcgggc gcatcggctc
     6601 cggatattct ggtgcagaat gtggtggcac gtttgcagca gctgggcggt ggtgaagcca
     6661 ttccgctgga aggccgtgaa gaaaacattg ttttcgaagt gccgaaagag ctgcgtgtcg
     6721 atattcgtga agtcgattaa gtcattagca gcctaagtta tgcgaaaatg ccggtcttgt
     6781 taccggcatt ttttatggag aaaacatgcg tttacctatc ttcctcgata ctgaccccgg
     6841 cattgacgat gccgtcgcca ttgccgccgc gatttttgca cccgaactcg acctgcaact
     6901 gatgaccacc gtcgcgggta atgtctcggt tgagaaaact acccgcaatg ccctgcaact
     6961 gctgcatttc tggaatgcgg agattccgct cgcccaaggg gccgctgtgc cgctggtacg
     7021 cgcaccgcgt gatgcggcat ctgtgcacgg cgaatcggga atggctggct acgactttgt
     7081 tgagcacaac cgaaagccgc tcgggatacc ggcgcttctg gcgattcggg atgccctgat
     7141 gcgtgcacca gagcctgtta ccctggtggc catcggcccg ttaaccaata ttgcgctgtt
     7201 actttcacaa tgcccggaat gcaagccgta tattcgccgt ctggtgatca tgggtggttc
     7261 tgccggacgc ggcaactgta cgccaaacgc cgagtttaat attgctgccg atccagaagc
     7321 tgctgcctgt gtcttccgca gtggtattga aatcgtcatg tgcggtttgg atgtcaccaa
     7381 tcaggcaata ttaactcctg actatctctc tacactgccg cagttaaacc gtaccgggaa
     7441 aatgcttcac gccctgttta gccactaccg tagcggcagt atgcaaagcg gcttgcgaat
     7501 gcacgatctc tgcgccatcg cctggctggt gcgcccggac ctgttcactc tcaaaccctg
     7561 ttttgtggca gtggaaactc agggcgaatt tacctcaggc acgacggtgg ttgatatcga
     7621 cggttgcctg ggcaagccag ccaatgtaca ggtggcattg gatctggatg tgaaaggctt
     7681 ccagcagtgg gtggctgagg tgctggctct ggcgtcgtaa cctgtcacat gttattggca
     7741 tgcagtcatt catcgactca tgcctttcac tgatatccct ccctgtttat cattaatttc
     7801 taattatcag cgtttttggc tggcggcgta gcgatgcgct ggttactctg aaaacggtct
     7861 atgcaaatta acaaaagaga atagctatgc atgatgcaaa catccgcgtt gccatcgcgg
     7921 gagccggggg gcgtatgggc cgccagttga ttcaggcggc gcttgcatta gaaggcgtgc
     7981 agctgggcgc ggcgctggag cgcgaaggat cttctttact gggcagcgac gccggtgagc
     8041 tggctggtgc agggaaaaca ggcgttaccg tgcaaagcag cctcgatgcg gtgaaagatg
     8101 attttgatgt gtttatcgat tttacccgtc cggaaggtac gctgaaccat ctcgcttttt
     8161 gtcgccagca tggcaaaggg atggtgatcg gcactacggg gtttgacgaa gccggtaaac
     8221 aggcgattcg tgacgccgcc gccgatattg cgattgtctt tgccgccaat tttagcgttg
     8281 gcgttaacgt catgcttaag ctgctggaga aagcagccaa agtgatgggt gactacaccg
     8341 atatcgaaat tattgaagca catcatagac ataaagttga tgcgccgtca ggcaccgcac
     8401 tggcaatggg agaggcgatc gcccacgccc ttgataaaga tctgaaagat tgcgcggtct
     8461 acagtcgtga aggccacacc ggtgaacgtg tgcctggcac cattggtttt gccaccgtgc
     8521 gtgcaggtga catcgttggc gaacataccg cgatgtttgc cgatattggc gagcgtctgg
     8581 agatcaccca taaggcgtcc agccgcatga catttgctaa cggcgcggta agatcggctt
     8641 tgtggttgag tggtaaggaa agtggtcttt ttgatatgcg agatgtgctt gatctcaata
     8701 gtttgtaacc acaaaatatt tgttatggtg caagaataac acatttaatt
//
LOCUS       DAZGAN020000165         8638 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGAN020000165 DAZGAN020000000
VERSION     DAZGAN020000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.165"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(241..447)
                     /locus_tag="ACHKYO_RS22865"
                     /old_locus_tag="pgaptmp_004573"
     CDS             complement(241..447)
                     /locus_tag="ACHKYO_RS22865"
                     /old_locus_tag="pgaptmp_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HGU4431283.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            760..849
                     /gene="kdpF"
                     /locus_tag="ACHKYO_RS22870"
                     /old_locus_tag="pgaptmp_004574"
     CDS             760..849
                     /gene="kdpF"
                     /locus_tag="ACHKYO_RS22870"
                     /old_locus_tag="pgaptmp_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HGU4431284.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            849..2522
                     /gene="kdpA"
                     /locus_tag="ACHKYO_RS22875"
                     /old_locus_tag="pgaptmp_004575"
     CDS             849..2522
                     /gene="kdpA"
                     /locus_tag="ACHKYO_RS22875"
                     /old_locus_tag="pgaptmp_004575"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HGU4431285.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2545..4593
                     /gene="kdpB"
                     /locus_tag="ACHKYO_RS22880"
                     /old_locus_tag="pgaptmp_004576"
     CDS             2545..4593
                     /gene="kdpB"
                     /locus_tag="ACHKYO_RS22880"
                     /old_locus_tag="pgaptmp_004576"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HGU4431286.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4602..5174
                     /gene="kdpC"
                     /locus_tag="ACHKYO_RS22885"
                     /old_locus_tag="pgaptmp_004577"
     CDS             4602..5174
                     /gene="kdpC"
                     /locus_tag="ACHKYO_RS22885"
                     /old_locus_tag="pgaptmp_004577"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HGU4431287.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5167..7851
                     /gene="kdpD"
                     /locus_tag="ACHKYO_RS22890"
                     /old_locus_tag="pgaptmp_004578"
     CDS             5167..7851
                     /gene="kdpD"
                     /locus_tag="ACHKYO_RS22890"
                     /old_locus_tag="pgaptmp_004578"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HGU4431288.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQELTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7848..8525
                     /gene="kdpE"
                     /locus_tag="ACHKYO_RS22895"
                     /old_locus_tag="pgaptmp_004579"
     CDS             7848..8525
                     /gene="kdpE"
                     /locus_tag="ACHKYO_RS22895"
                     /old_locus_tag="pgaptmp_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HGU4431289.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1830 a         2217 c         2484 g         2107 t
ORIGIN      
        1 gctcatcctt gttttatcgg gttatttgtc tgcttccagg caaaacccta cagatttata
       61 agaaagcctt aagtgagata tctgtcagat aagaacaatt aggaaaatat ttatatatat
      121 ttcatgaata tatgtttcga ttggtataac gatgtctaaa tattcctctg aacatcttcc
      181 tgatacaaaa aaccgccagc gtttccgctg gcggttttca atgtattcaa aactcagcaa
      241 tcaataaaaa tcaccagttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt
      301 tttcgaccag acgcgatgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat
      361 gaaaggcagc gccagaacgc ccgctgcaac cgcataagta cggcgtaaaa agataagcca
      421 tgcaggatat tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa
      481 tggcaccgga aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt
      541 tactactcat ccgaccactt atttttgctt attgatggtt tatttacatt catcctgtaa
      601 ttaagttaca caaaagttaa attaatacta aacattagtt aaatcatggc ttttgccatt
      661 tttatacttt ttttacaccc cgcccgcaga tttttgcgaa atctttgcag ccagaattct
      721 acccttccgg tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgataacc
      781 ggcgtattgc tggtgttttt attactgggt tatctggttt atgccctgat caatgcggag
      841 gcgttctgat ggctgcgcaa gggttcttac tgatcgccac gtttttactg gtgttaatgg
      901 tgctggcgcg tcctttaggc agcgggctgg cgcggctgat taatgacatt cctcttcccg
      961 gtacaacggg cgttgagcgc gtactttttc gcgcacttgg cgtctctgac cgtgagatga
     1021 actggaagca atatctctgt gccattctcg gcctgaacat gctggggctg gcggtgctgt
     1081 tttttatgtt gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt
     1141 cgtgggatct ggcgctgaat accgccgtca gctttgtcac gaataccaac tggcaatctt
     1201 acagcggtga aaccacgttg agctatttca gccagatggc tggcttaacg gtgcaaaact
     1261 ttctttctgc cgccagcggg attgcggtga tttttgccct catccgtgcg tttacccgcc
     1321 agagcatgag cacactcggg aatgcctggg tcgatctgct acgcatcacg ttatgggtgc
     1381 tcgtccctgt ggcgttgttg atcgcactgt tttttattca acaaggtgcg ctgcaaaact
     1441 ttcagcctta tcaggctgtg aataccgttg aaggagcgca acagctgtta cccatggggc
     1501 ctgtagcttc tcaggaagcg atcaagatgc tcggtactaa cggcggtggc ttctttaatg
     1561 ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac caacttcgtg cagatgctgg
     1621 cgatcttctt gatcccaacg gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc
     1681 gccaggggcg catgttgctg tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg
     1741 tgatgtgggc agaagttcag ggtaatcctc atctgctggc gctcggcgca gacagcagca
     1801 tcaatatgga aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg
     1861 tgactacggc ggcttcctgt ggcgcggtga ttgcgatgca tgattcgttt accgctctcg
     1921 gtggcatggt gccgatgtgg ctgatgcaaa ttggtgaagt ggtgttcggc ggtgtcggtt
     1981 ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga
     2041 ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt acgcgagatg aaactgactg
     2101 cactggcgat tctggttacc ccgacgctgg tgctgatggg cgcggcgctg gcaatgatga
     2161 ccgacgccgg acgtagcgcc atgctcaacc ctggcccgca tggttttagc gaagtgctgt
     2221 acgccgtgtc atccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact
     2281 ctccgttctg gaactgttta ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta
     2341 tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg
     2401 gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg
     2461 ttggcgcact gacctttatc cctgccctgg cgcttggtcc agtggcggaa tatctctcct
     2521 gatgatattg agtgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca
     2581 cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc
     2641 aatccggtga tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg
     2701 atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag cggctggctg
     2761 tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg
     2821 caggccaaca gtcttaaagg ggtgaaaaaa actgcctttg cccgcaagct gcgtgagccg
     2881 aaatatggcg ctgcggcgga caaagttcct gccgatcaac tgcgtaaagg tgacatcgtg
     2941 ctggtagaag ctggcgatat tatcccctgc gatggtgaag tcattgaagg cggcgcatcg
     3001 gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga tccgtgaatc cggcggcgat
     3061 tttgcctccg tcaccggcgg cacgcgtatt ctttctgact ggctggtgat tgagtgtagc
     3121 gttaaccccg gtgagacatt tctggatcgg atgatcgcga tggtggaagg cgcacagcga
     3181 cgcaaaacac cgaacgagat tgccctgact atcctgctga ttgctctgac catcgtcttt
     3241 ttactggcaa ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc cgtgagtgta
     3301 acggtactgg tggcgctgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca
     3361 gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga atgtgattgc caccagcgga
     3421 cgtgcagttg aagcggcagg tgacgttgac gttctgctac tggataaaac cggcaccatc
     3481 acactcggta accgtcaggc gtcggagttt atccctgcac agggcgtgga tgaaaaaacg
     3541 ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg ccgcagtatt
     3601 gtgatcctcg ccaagcaacg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc
     3661 acctttgtac cgtttactgc gcaaagccgg atgagcggga tcaacatcga caaccgcatg
     3721 atccgtaaag gttctgtcga tgccattcgc cgccatgttg aggctaacgg tggtcacttc
     3781 cctaccgatg ttgatcaaaa agtcgatcag gttgcgcgtc agggggccac gccgctggtg
     3841 gtggtggaag gttcccgtgt gctgggcgtt attgcgctga aagatatcgt caaaggcggt
     3901 attaaagagc gcttcgccca gctgcgcaaa atgggcatta aaacggtgat gattaccggc
     3961 gataaccgtc tgactgccgc cgccattgct gcggaagcgg gcgtcgatga ttttctcgcc
     4021 gaagcgacac cggaggccaa gctggcattg attcgtcagt atcaggcgga aggccgtttg
     4081 gtggcgatga ccggcgacgg caccaacgat gctccagcgc tggcgcaggc agatgtcgcg
     4141 gtggcgatga actccggtac tcaggcggcg aaagaagcgg gcaatatggt cgatctcgac
     4201 tctaacccga ccaagctgat cgaggtggta catattggca aacagatgct gatgacccgt
     4261 ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg
     4321 gcggcgttcg cggcaacgta tccgcagtta aatgcgctga acatcatgcg ccttcattcg
     4381 cccgactccg ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt
     4441 cctctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt
     4501 aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt
     4561 gatttactgc tgaccgtttg cggtctggtg tgaggtttac catgcgtggc ttacgtccgg
     4621 cattatcgac atttatcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca
     4681 ccgtgctggg gcaatggtgg tttccctggc aggccaacgg ttcgttgatt cgtgaaggtg
     4741 atacggtgcg gggttcggca ttaatcgggc agaattttac cggcaacggc tattttcagg
     4801 gtcgcccgtc ggcaacggca gaaatgccct ataatccaca ggcttctggc gggagcaatc
     4861 tggcggtcag taaccctgag ctggataaac aaatagccgc acgcgttgct gcattacggg
     4921 ccgctaaccc ggatgccagt acgaacgttc cggttgaact ggtgacggca tcggcaagcg
     4981 ggctggacaa taatatcacc ccgcaagcgg cggtctggca aatcccacgc gtggcgaaag
     5041 cgcgtaatct cagcgttgaa cagctcacgc aactgatcgc aaaatacagc caacaaccgc
     5101 tggtgaaata tatcggccag ccggtcgtca acattgttga actcaatctg gcgctggata
     5161 aacttgatga ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaaactgcc
     5221 gcgccgcatc gggggaagct gaaagttttc ttcggtgcct gtgcaggcgt cgggaagacc
     5281 tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggc
     5341 gtggtagaaa cccatgggcg gaaagatacc gccgccatgc ttgaagggct ggctgttctg
     5401 ccgcccaaac gccaggcgta ccgtgggcgg catatcagcg agtttgatct cgatgccgcc
     5461 ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt
     5521 tctcgtcatc ctaaacgctg gcaggatatc gaagaactgc tggaagctgg cattgatgtt
     5581 ttcacaaccg tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc
     5641 ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtagtg
     5701 ctggtggact tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt
     5761 gccgggcagg cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg
     5821 cgtgaactgg cactgcgccg tactgccgat cgcgtagatg agcaaatgcg cgcctggcgg
     5881 gggcatcctg gtgaagagaa agtgtggcac acgcgcgacg cgatcctttt atgcatcggg
     5941 cataacaccg gcagtgagaa actggtccgc gcggcggcgc ggttggcctc gcgcctgggt
     6001 agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggaaaaaaaa
     6061 cgtcgggcaa ttctcagcgc cttacgtctg gcgcaggagc tgggcgcgga gacggctaca
     6121 ctttctgatc cagcggaaga aaaagcagta gtgcgttatg cccgtgaaca taatctcggc
     6181 aagattattc tcggtcgccc ggcctcgcgc cgctggtggc gtcgggaaac gtttgctgac
     6241 cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc
     6301 gcccgcacga ttaacaacgc gccggatagc cgctctttta aagacaagtg gcgggtacag
     6361 attcagggat gcgtggttgc cgccgcgtta tgcgccgtta tcactttaat tgccatgcag
     6421 tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg
     6481 gtggcgctat tttatggacg ctggccttcg gtggttgcta ccgtcattaa tgtggtgagt
     6541 ttcgatctct tttttatcgc cccacgcggc acgctcgctg tctctgatgt gcaatatctg
     6601 ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgcgggtgtg
     6661 cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt atatgaaatg
     6721 tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag cgaacaattt
     6781 attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg
     6841 cagccgttaa cacatccgca aggaatgacg ccatgggacg atgccatcgc gcagtggagt
     6901 tttgataaag gcctgcctgc gggtgcgggc accgacacgt tacccggcgt gccgtaccag
     6961 attttgccgc taaaaagcgg cgagaaaacc tacggactgg tggtggtgga accggggaat
     7021 ctgcgtcagt tgatgatccc ggagcagcag cgcctgctag agacgtttac gctgttagtc
     7081 gccaatgcgt ttgagcggct gacgctgacc gccagtgaag aacaggcgcg gatggcaagc
     7141 gaacgtgaac agatccgcaa cgccctgctg gcggcgcttt cgcatgattt acgcacgccg
     7201 cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatcg
     7261 ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac ccgactggtg
     7321 aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg
     7381 ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg
     7441 cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg gccactcttt
     7501 gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa
     7561 attggtatcg atgcccacgt tgagggcgaa aatctacaac tggatgtctg ggataacggc
     7621 cccggtcttc cgccaggcca ggagctgacg atatttgata agtttgctcg cgggaataaa
     7681 gagtcggcag taccgggggt agggcttgga ctggcaattt gtcgggcgat agtggatgta
     7741 cacgggggca ctattaccgc gttcaaccga ccggaaggtg gtgcctgttt tcgtgttaca
     7801 cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg acaaacgttc
     7861 tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg
     7921 ggatgcgcgt ctatgaggcc gaaacgctgc aacgcggctt gctggaagcg gctacccgta
     7981 agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt gagtttatcc
     8041 gcgacctgcg ccagtggagc gcggtgccgg tgattgtgct ttccgcacgc agcgaagaga
     8101 gcgacaaaat cgccgcgctg gatgccggag cggatgatta tctgagtaag ccgtttggca
     8161 ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc accgccgcgc
     8221 ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt agccgcccgc gtgattcacc
     8281 ggggtgagga agaggtgcat ctcacgccga ttgagttccg cctgctggcg gtgctgctga
     8341 ataatgccgg gaaagtgctc acccagcgcc agttactcaa tcaggtgtgg gggccaaacg
     8401 cggtcgaaca cagtcactat ttgcgtattt atatgggaca tctgcgacaa aaactggaac
     8461 aggatcccgc tcgcccccgc catttcatta ctgaaaccgg tattgggtat cggtttatgc
     8521 catgaatgtt tattcaataa atgcgcttaa ataattatta atttattatt aaacctgcca
     8581 aaaatattat tatttggcag gtttaatttc ttaacgcaaa tataaaacac aaaattac
//
LOCUS       DAZGAN020000166         8307 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGAN020000166 DAZGAN020000000
VERSION     DAZGAN020000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.166"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(117..452)
                     /gene="ymgD"
                     /locus_tag="ACHKYO_RS22900"
                     /old_locus_tag="pgaptmp_004580"
     CDS             complement(117..452)
                     /gene="ymgD"
                     /locus_tag="ACHKYO_RS22900"
                     /old_locus_tag="pgaptmp_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HGU4431290.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(456..800)
                     /gene="ymgG"
                     /locus_tag="ACHKYO_RS22905"
                     /old_locus_tag="pgaptmp_004581"
     CDS             complement(456..800)
                     /gene="ymgG"
                     /locus_tag="ACHKYO_RS22905"
                     /old_locus_tag="pgaptmp_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HGU4431291.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(802..975)
                     /gene="ymgI"
                     /locus_tag="ACHKYO_RS22910"
                     /old_locus_tag="pgaptmp_004582"
     CDS             complement(802..975)
                     /gene="ymgI"
                     /locus_tag="ACHKYO_RS22910"
                     /old_locus_tag="pgaptmp_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HGU4431292.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1076..1261
                     /gene="ymgJ"
                     /locus_tag="ACHKYO_RS22915"
                     /old_locus_tag="pgaptmp_004583"
     CDS             1076..1261
                     /gene="ymgJ"
                     /locus_tag="ACHKYO_RS22915"
                     /old_locus_tag="pgaptmp_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HGU4431293.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            1222..1341
                     /locus_tag="ACHKYO_RS22920"
                     /old_locus_tag="pgaptmp_004584"
     CDS             1222..1341
                     /locus_tag="ACHKYO_RS22920"
                     /old_locus_tag="pgaptmp_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGU4431294.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            1376..1720
                     /locus_tag="ACHKYO_RS22925"
                     /old_locus_tag="pgaptmp_004585"
                     /pseudo
     CDS             1376..1720
                     /locus_tag="ACHKYO_RS22925"
                     /old_locus_tag="pgaptmp_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2092..2358)
                     /gene="minE"
                     /locus_tag="ACHKYO_RS22930"
                     /old_locus_tag="pgaptmp_004586"
     CDS             complement(2092..2358)
                     /gene="minE"
                     /locus_tag="ACHKYO_RS22930"
                     /old_locus_tag="pgaptmp_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HGU4431295.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2362..3174)
                     /gene="minD"
                     /locus_tag="ACHKYO_RS22935"
                     /old_locus_tag="pgaptmp_004587"
     CDS             complement(2362..3174)
                     /gene="minD"
                     /locus_tag="ACHKYO_RS22935"
                     /old_locus_tag="pgaptmp_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HGU4431296.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(3198..3893)
                     /gene="minC"
                     /locus_tag="ACHKYO_RS22940"
                     /old_locus_tag="pgaptmp_004588"
     CDS             complement(3198..3893)
                     /gene="minC"
                     /locus_tag="ACHKYO_RS22940"
                     /old_locus_tag="pgaptmp_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HGU4431297.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4413..4781
                     /gene="ycgJ"
                     /locus_tag="ACHKYO_RS22945"
                     /old_locus_tag="pgaptmp_004589"
     CDS             4413..4781
                     /gene="ycgJ"
                     /locus_tag="ACHKYO_RS22945"
                     /old_locus_tag="pgaptmp_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HGU4431298.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            complement(4901..5302)
                     /gene="pliG"
                     /locus_tag="ACHKYO_RS22950"
                     /old_locus_tag="pgaptmp_004590"
     CDS             complement(4901..5302)
                     /gene="pliG"
                     /locus_tag="ACHKYO_RS22950"
                     /old_locus_tag="pgaptmp_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HGU4431299.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5544..5837
                     /locus_tag="ACHKYO_RS22955"
                     /old_locus_tag="pgaptmp_004591"
     CDS             5544..5837
                     /locus_tag="ACHKYO_RS22955"
                     /old_locus_tag="pgaptmp_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HGU4431300.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5909..6568
                     /gene="ycgM"
                     /locus_tag="ACHKYO_RS22960"
                     /old_locus_tag="pgaptmp_004592"
     CDS             5909..6568
                     /gene="ycgM"
                     /locus_tag="ACHKYO_RS22960"
                     /old_locus_tag="pgaptmp_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGU4431301.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            6660..7106
                     /gene="ycgN"
                     /locus_tag="ACHKYO_RS22965"
                     /old_locus_tag="pgaptmp_004593"
     CDS             6660..7106
                     /gene="ycgN"
                     /locus_tag="ACHKYO_RS22965"
                     /old_locus_tag="pgaptmp_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HGU4431302.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7313..8221)
                     /gene="hlyE"
                     /locus_tag="ACHKYO_RS22970"
                     /old_locus_tag="pgaptmp_004594"
                     /pseudo
     CDS             complement(7313..8221)
                     /gene="hlyE"
                     /locus_tag="ACHKYO_RS22970"
                     /old_locus_tag="pgaptmp_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT         2142 a         1847 c         1920 g         2398 t
ORIGIN      
        1 ctgggtgata atggctacaa tgacaccgca gtgatggtgg gcctgaaata taagttctga
       61 tcccgccgtt agctaaaaaa ccgcgtcgta ttcatcgacg cggtacatat gaaatattat
      121 tttttgccga tagcacgcat ggtgtcatca attgccgtga tcaacagcat ttgcgggtct
      181 ttagcgcaaa cctgattcag tttttctacc actttggcgc tcagttccgg agattgctca
      241 atttttaaat cacggctggc aacgctggca ttacccatta ccgcaacaat ttctgcaacc
      301 tgtgcgctgt cagtttttgc catttcgttg gcttctgcgc aagtaatata ggtttctgac
      361 ggcaaaccgt ttttaatatt gtagtcctgc gcccaggtca ttggtgcgaa aacaaacagg
      421 cccgccagta aagcaaattt tttcatcatc attccttatt tcattttacc cagaattgca
      481 ccacccgtac cgccaatcac ggcaccttta atcgcccctt cgaggccatt gccggtcaga
      541 acgccagtga cagcaccaac ggcggcaccc acttttgcac ctttacgcgc atttttaccg
      601 tcgcggcctt tttctgttac tgcaccaaca ccagcgccaa cagctgcacc tttcagtacg
      661 ccattaacac cattgccagt aagtaaacca acgcctgcgc ctagcaatgc acctttcgtg
      721 gtgcggttca tatccgccat cgctggcgtg gagcagaaca atgctgagat aagcccgaag
      781 gcaagtattt ttttcttcaa cttagatgtc cggtattaag taagttgcac acacaataat
      841 ttcgtcttca attaagatct gcttaactaa agaacgctcg ctattattca gataattcaa
      901 aatgagcgtg gctgtgatga taggaattat gttttttacg tgaatgagaa taatcttaaa
      961 tgaggaataa ctcattgatt gacaatattt ttattcaaga agtgtcattg actgttaacg
     1021 caatgttgta aaggtaagat aatctgattt atcaatatca ttgtgtgatt tttatgtgag
     1081 cagaagatat tcatcagcaa cgattacatt agtcatttta ttttgccgac ggcctcattg
     1141 tcgaaagata agcgtacgac agtattatct gaaaagagtg attttttatc caactacact
     1201 tcagcgcact gcgtgtaaaa aatgcctctt tcttatgcgg gatatcatca tttcatcatg
     1261 atgtctttgg tgagcggtga acacaataca cctgcgctgt ctcttcagga tgaatcccct
     1321 ggtctggtgc ctgcgggctg atgttgcagc agagcttagg tcacttagac gctactatca
     1381 tttatccaat ggcatggaat cgaaatcagt cgatacccgc agtatatatc gtgaactggg
     1441 tgcaacgctg agttacaaca tgcgtctggg gaacggtatg gaaattgaac cctgcctgaa
     1501 ggcggctgtg cgcaaagaat ttgtcgatga taatcgggtg aaagtgaata gtgacggtaa
     1561 ttttgtcaat gatttgtcgg gcagacgtgg aatataccag gcaggtatta aagcctcatt
     1621 cagcagtacg ttaagcgggc atttcggggt ggggtatagc catggtgccg gtgtggagtc
     1681 cccgtggaac gcggtggctg gtgtgaactg gtcgttctga ccatcaacga ttaaactgcg
     1741 cttcggcgca gttttcgttt acaggatgtt gaaagagaaa attctggggc aaaaaaagcc
     1801 cgccagttac ggtgggaaac ctcatcctat gggagaacaa tgaataatga aattgcgggg
     1861 ttatcatctc ccagtatatc cacactaaca acagggttat ttactcaacc aggcataaac
     1921 attttgttct gtgcgtggga acagccttaa ggtgtaaagg gggaggtgga aatagcaatg
     1981 aggagtatca gcaagaatac tcgccgcttt accacaacgt ggatgagagg gatgaaaaac
     2041 tcaaggcaga aatgactctg ccttgaagat aaatgcgttt ttacagcggg cttatttcag
     2101 ctcttctgct tccggtaagg tcacgttcag ctcaagaata gaaatatcgc catctttttg
     2161 ctcaagctgt acggttacca tctcaggatc aatttgtacg tatttacaaa tgacctcaag
     2221 aatatcttta cgcaactgcg gcagataatg cggttctgca tcgctgcgac ggcgttcagc
     2281 aacaataatc tgcagccgtt cttttgcaat gttggctgtg tttttcttcc gcgagagaaa
     2341 gaaatcgagt aatgccataa cttatcctcc gaacaagcgt ttgaggaagc ctttcttctc
     2401 ttcttcaatg aagcggaaag gacgttcttc tcccaacaga cgttctacgg tatctgcgta
     2461 ggctttaccc gcatcggcgt taatgtcgag aatgaccggt tcaccctggt tagaggcgcg
     2521 caatactgat tgatcctctg ggatcacgcc gacgagtttg atgcgcagga tctccagcac
     2581 atcttccatg ctcagcatgt cacctctgct tacgcggcct gggttatagc gcgttaacag
     2641 caggtgctct ttaataggct cttcgccatt ttctgcgcgg cgtgatttcg acgccagaat
     2701 gcctaaaata cggtcagagt cgcgtactga ggagacttcc gggttggtgg taataatggc
     2761 ttcgtctgca aaatagagtg ccattaacgc accggtttca atccctgccg gggagtcaca
     2821 aacgataaat tcaaaatcca tcgctttcag atcatcaaga actttggcga ccccttcacg
     2881 ggtgagggca tctttatcgc gtgtttgcga tgccggaaga atatagagat tttcagtacg
     2941 cttatcttta attaacgcct gatttagcgt tgcatcgccc tgaatgacgt tgacgaaatc
     3001 gtaaacgacc cggcgttcac aacccataat caggtcgaga ttacgcaggc cgatatcaaa
     3061 atctatcacg acagttttct ttcccttctg ggccaaacca gtggcgatgg ccgcgctgga
     3121 ggttgtctta ccaacacccc ctttgcccga agtaacaaca ataatgcgtg ccatagaaat
     3181 tccttgttaa aaagggatca atttaacggt tgaacggtca aagcgttttc gactaactgc
     3241 agtcgcgccg ctttgccata aaattctgct gggatttgat cactcagcca gtattcacct
     3301 gcgatggaca ccagttccgc catcaggttc gtacaaaata tttgcgtttc ccggtcacca
     3361 cttgcccctg caagcgcacg accgcgcatc atgccataga catgaatgtt cccatcggca
     3421 atcaattcgg ccccagcgct aacgtggctt gtaacaatca gatcacattg tggagcataa
     3481 atacgctgac cggaacgcac cggggtatct attaaacgcg tttttgtgac cggcgttgta
     3541 ttttgcgctg gagcctgtgg tgcgggagct ggacgcggcg ctttttcctt tccttccgtc
     3601 aggataggca gccccatctt ttcaatttcg gctttaagtt gcgcatcttt gcagccactt
     3661 acgccaataa cccgcaaacc ggttgccgaa accgccttat gcatcgctga ccagtttacc
     3721 gggtcttcca gtgcactgac gttgagtaca acgggggcat gttttaaaaa tgcgggggcc
     3781 tgagcgattt tgtcttccag cgcctgatgg ataaccttag gttctgcctc atgcagatga
     3841 accacagata aagtgaagct actgccttta agctcgattg gcgtgtttga catcctggcc
     3901 ttactcaatt agctattaat catcgccagc gcgcgatgat gttccgaaga ctataaggca
     3961 tgttatagtc tggattatat tgaggcaagt caccctccca tttattcaga gtaaaagtct
     4021 attctgtgat aaatggcgct gattcatagc ttaaaaaata cccttgtcaa tcaacccatt
     4081 gccgtcgtac ttttgattgt tcttatttac gcttcctttt tccgcaccct aatcgtgaca
     4141 gcatctccag acctgtttaa atatccagta ttgaggcaag tgtatttttt caccacgcca
     4201 ggctttctta actttccatt ttttgcggca tcattaaaga acagataccc gttatcttaa
     4261 tatccgccac agaattgctc tatcatagaa accgcagaag acgtctgata cttctgtcag
     4321 gtcatcgtaa ctcgtccagg tcagcggttt tgattacgga ttgatgatga aatgagcata
     4381 agtgttgtac ggttggatta tcaggatgtg ctatgaatat gatgcgtatt ttttatatcg
     4441 gattgtcagg tgtggggatg atgttctcat caatggcgtc tggacatgac gctggcggac
     4501 ttcaatctcc ggcgtgcggc gttgtttgcg acccgtatat ttgtgtgaac tcagatggca
     4561 tttccccaga gttaacaagg aaatatctcg gcgaaaaagc cgctgaaaac ttacaatcat
     4621 tacaaggcta cgatcccagc gaatttacat tcgctaacgg tgtattttgc gatgttaaag
     4681 aaaaattatg tcgtgatgat cgttattttg gtgtggatgg taagcggagt ggaaaaatca
     4741 atcaaatcac cacaaagatg ttatttatgt gtcgtgagtg atgccttgtt tacattatgt
     4801 tctattcggc attgtctgct attcgtcatt aacctcttga gcgacagaga ggagtggggc
     4861 gttaacgttt atgacgggga gagtccccgt cggttgacat ttacttaatt tgaatatcga
     4921 cgttatattt tttagttttg tttttacggg cgtcgtttcg ggtttgcagc actcgcagct
     4981 catatttgcc agaagcaggg agtgagtatt gtccgtgact atcgagttct ggtgaatatc
     5041 tggataaatc aactgaatca tcgattcctg gcccgaagag gtaagtatca gcgccttcat
     5101 tagagatact cacatgcact ttctgacctt ttttggcata gaaggtatat gtatcgtaat
     5161 cgtatccctt tatttcgccg gagtactgag cgctgctatg gcctttgctg aactcaacat
     5221 tgacattctt acccgcagca aagctcgtgg aggttagcag tgcaagcagc aatacagcct
     5281 tactgatgct cttgattttc attggtagtc tcctgttcag aatgagtacg atgaactgtt
     5341 ataatataac aatcccttac gggagaattc cagtggcttt tctgcgttcg gtaaaatccc
     5401 ggcgcggcat tttttgccgg ggggattact tataaagggg catggactca acaaaccgta
     5461 gaaatctaca gctggctggc ttgtggcgcg ggtttcattg atgttagctt atgccaaaac
     5521 ctggcatctt aaagagtaaa agtatgtttt gtgtgattta tcgaagcagc aagcgtgacc
     5581 agacctattt atatgtcgaa aaaaaagacg atttttcgcg tgttcctgag gaactgatga
     5641 aaggttttgg tcagcctcag ttagcgatga ttctgccgtt ggatgggcgg aagaaactgg
     5701 tcaatgctga tattgaaaag gttaaactgg cattaaccga gcaaggttac tatttgcagt
     5761 taccgccacc acccgaagat ttgctgaagc aacatctttc cgtcatgggg cagaaaacag
     5821 acgacactaa caaataaccg atatccggcg gtggcattat ctttgtcggc gcgggttttc
     5881 atatccacga taaggtgagg ggaacgttat gtatcaacat cacaactggc aaggtgcgct
     5941 gctggattat ccggtgagta aagtagtctg tgttggcagt aactatgcca aacatattaa
     6001 agagatgggc agcgcagtgc ccgaagagcc agtgctgttt attaaaccag aaacggcact
     6061 gtgcgatctg cggcagccat tggcgatccc atccgatttc ggttcagttc atcatgaagt
     6121 cgaactggcg gtgttgattg gcgcgacgct gcgtcaggct acggaagagc atgtccgcaa
     6181 agccattgcc ggttatggcg tggcgctcga tctgacattg cgtgatgttc agggaaaaat
     6241 gaagaaagcc gggcagccgt gggaaaaggc taaagcgttt gataactctt gtccgctttc
     6301 cgggtttatt cccgcggcgg aatttaccgg cgacccgcaa aatacaacgc tgggcctgag
     6361 cgtaaacggc gagcaacgtc agcaaggtac gactgcggac atgatccata aaattgttcc
     6421 gctgatcgct tatatgagca agttttttac cctcaaggcc ggtgacgttg tgctgacagg
     6481 cacgcctgat ggcgtcggcc cgttgcaaag cggtgatgag ctgacagtca ctttcgatgg
     6541 gcattctttg gcaactcgcg ttttgtaata ctttttgccg cctgaaagcg gcggcaaaac
     6601 ttgcatcgct gtgccagact ggttataagg tgcgttttaa cgtaatggcg gaacacctga
     6661 tgagcgatgt acctttctgg caaagtaaaa ccctggacga aatgagcgat gcggaatggg
     6721 agtcgttatg tgatggttgc ggtcagtgtt gcctgcataa actgatggat gaagacaccg
     6781 acgaaatcta cttcactaac gtcgcctgtc gccagctcaa tattaaaacc tgtcaatgtc
     6841 ggaactacga acgtcgtttc gagtttgaac ccgactgcat taaattaacc cgtgaaaatc
     6901 tgccaacatt cgaatggctg ccaatgacct gcgcttatcg tttgctggcg gaaggtaaag
     6961 atttacctgc gtggcatccg ctacttactg gttcgaaagc ggcaatgcat ggtgaacgta
     7021 tctctgtgcg tcatatcgca gtgaaagaat cagaagtgat tgactggcag gatcatatct
     7081 taaataaacc tgactgggcg cagtgataaa tttaatagat gttgatgggg gctttcttaa
     7141 tatacgtctg gatgctcgcc atttcgtagc cctgtttttg aaagcgctct gtcataatga
     7201 gagttacccg ataccttatt atcaacaggt aactctcaat ctttaaaaaa cgtctgtgtt
     7261 tttaaatgtg ataaacctta taccttgagt acatggagag aataatcgct tatcagactt
     7321 caggtacctc aaagagtgtc tttttaccgt gtcttttctg atactcatta caggtgttaa
     7381 tcattttttg ccgcttcttt tagcaaagaa agcattaaat catcataatc aacatagaat
     7441 ctggttgttt cagtttccgt ttttatctcc ccgatggcgg ctatttcggt ggttaatttc
     7501 aatttggcgg catcgatatc tttattcgct tgtttaaccg tgttagacag ggtggtaaag
     7561 aaattctgca cagattttaa cttgttcttc aattctggaa tcagtttccc ttcaactacg
     7621 cccgcagcaa tagaatagga aatgattaat ccaaatggac cggcgacgac accggctgcg
     7681 gcaccggcat acgcttcctt cctgatttta tctacctgtg actggaaaag ctgctttttt
     7741 ctgaaaaatc attggttaac tggctatcta acgccagcag tttcccggaa gcgttgttga
     7801 aactttgtga gcttaccagc agggattttt gcgcttcatt cagcttcgtg atgccgtcat
     7861 ccagtacctt aatgagaatg tctttctggg cggatgcttt cttctcattg tactcatcaa
     7921 atagaaaaat atacgctgcg agcaattgcg tcgcaacacc acaccattca tacactgttt
     7981 gggttgcttc aaaatactta tcctggctat ccataagtaa ggttttaata tcgccgacta
     8041 aaacggaggc tgcctgtgaa tactcctgtt taaagcgact taactctttt atggtttcat
     8101 caaaggtctg ccaggggatg acctgatcga gatatttatt ataaagatct aatgctccat
     8161 ctgcagtttc gattgcgttt ttaactactt ctaccgtttt atctgcaacg atttcagtca
     8221 taatcattcg cctctttaaa tatataaatt gtaatgaaac tcctgtttta caactattaa
     8281 taaattttac ttcatctaat tcatagt
//
LOCUS       DAZGAN020000167         8139 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGAN020000167 DAZGAN020000000
VERSION     DAZGAN020000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.167"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..173
                     /locus_tag="ACHKYO_RS22975"
                     /old_locus_tag="pgaptmp_004595"
     CDS             <1..173
                     /locus_tag="ACHKYO_RS22975"
                     /old_locus_tag="pgaptmp_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGU4431303.1"
                     /translation="KKQDDNLEHFISVTEHPSGSDLIYYPEGNNDGSPEAVIKEIKEW
                     RAANGKSGFKQG"
     gene            235..378
                     /locus_tag="ACHKYO_RS22980"
                     /old_locus_tag="pgaptmp_004596"
     CDS             235..378
                     /locus_tag="ACHKYO_RS22980"
                     /old_locus_tag="pgaptmp_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HGU4431304.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGL
                     ATQ"
     gene            843..983
                     /locus_tag="ACHKYO_RS22985"
                     /old_locus_tag="pgaptmp_004597"
     CDS             843..983
                     /locus_tag="ACHKYO_RS22985"
                     /old_locus_tag="pgaptmp_004597"
                     /inference="COORDINATES: protein motif:HMM:NF020569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="HGU4431305.1"
                     /translation="MYEWDSQHGELEGYRASDGQHLGSFDPKTGNQLKGPDPKRNIKK
                     YL"
     gene            993..1250
                     /locus_tag="ACHKYO_RS22990"
                     /old_locus_tag="pgaptmp_004598"
     CDS             993..1250
                     /locus_tag="ACHKYO_RS22990"
                     /old_locus_tag="pgaptmp_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="HGU4431306.1"
                     /translation="MGLKLHINWFDKTTEEFKGGEYSKDFGDDGSVIERLGMPLKDNI
                     NNGWFDVIAEWVPLLQPYFNHQIDISGNEYFVSFDYRDGDW"
     gene            1270..1536
                     /locus_tag="ACHKYO_RS22995"
                     /old_locus_tag="pgaptmp_004599"
     CDS             1270..1536
                     /locus_tag="ACHKYO_RS22995"
                     /old_locus_tag="pgaptmp_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGU4431307.1"
                     /translation="MKVELKKSISDYTEAEFLELVKKICRAEGATEEDDNKLVREFER
                     LTEHPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
     gene            1598..1741
                     /locus_tag="ACHKYO_RS23000"
                     /old_locus_tag="pgaptmp_004600"
     CDS             1598..1741
                     /locus_tag="ACHKYO_RS23000"
                     /old_locus_tag="pgaptmp_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HGU4431308.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGWTVSPSSTAELTGL
                     ATQ"
     gene            2270..3184
                     /locus_tag="ACHKYO_RS23005"
                     /old_locus_tag="pgaptmp_004601"
     CDS             2270..3184
                     /locus_tag="ACHKYO_RS23005"
                     /old_locus_tag="pgaptmp_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HGU4431309.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQ
                     PHALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASA
                     SALRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLS
                     ASARRSVDKNYGLGRNCYLFEKGRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQATRGRKGGSKSKRGTVAT
                     SARTLKPWEKLGISRAWYYQLKKRGLVE"
     gene            complement(3401..3676)
                     /locus_tag="ACHKYO_RS23010"
                     /old_locus_tag="pgaptmp_004602"
     CDS             complement(3401..3676)
                     /locus_tag="ACHKYO_RS23010"
                     /old_locus_tag="pgaptmp_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HGU4431310.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRLQSLINELEKLPRPPAKPQNH"
     gene            3855..6074
                     /gene="mobQ"
                     /locus_tag="ACHKYO_RS23015"
                     /old_locus_tag="pgaptmp_004603"
     CDS             3855..6074
                     /gene="mobQ"
                     /locus_tag="ACHKYO_RS23015"
                     /old_locus_tag="pgaptmp_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HGU4431311.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTR
                     KRGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     ETVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACVMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            6307..8052
                     /gene="col"
                     /locus_tag="ACHKYO_RS23020"
                     /old_locus_tag="pgaptmp_004604"
     CDS             6307..8052
                     /gene="col"
                     /locus_tag="ACHKYO_RS23020"
                     /old_locus_tag="pgaptmp_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="HGU4431312.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMSGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKES
                     KRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEV
                     SKDPELSKQFNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNL
                     RITTPKRHIDIHRGQ"
BASE COUNT         2141 a         1816 c         2218 g         1964 t
ORIGIN      
        1 aaaaaaaaca ggatgataac ctcgagcatt ttataagtgt tactgagcat cctagtggtt
       61 ctgatctgat ttattaccca gaaggtaata atgatggtag ccctgaagct gttattaaag
      121 agattaaaga atggcgagct gctaacggta agtcaggatt taaacagggc tgaaatatga
      181 atgccagttg tttaaggatg aatgactggc attctttcac aacaaggagt cgttatgaaa
      241 aaaataacag ggattatttt attgcttctt gcagtcatta ttctggctgc atgtcaggca
      301 aactatatcc gggatgttca gggcgggacc gtatctccgt catcaacagc tgaagtgacc
      361 ggattagcaa cgcagtaacc cgaaatcctc tttgacaaaa acaaagcgtg tcaggctgat
      421 tctgatgcgc tttttttgaa atgtcacaaa agttccatgt gggagatggg atctaaaatc
      481 cctgtgcaga accttccatc cggtgggaga aaacttgtca ttttgacctg ttcgccattc
      541 ggggggggcg cttggaaaac ggaaattttc ccacgctaag agaattgagt tgaacgagtg
      601 gttacatcta ggccagctaa ctcactgaac gtaaaggctg tacgaaatgt tccgaccgtc
      661 cgataaccat ttggcttact tccgtacatt tccttgcgta gatgtacaga attgtacgat
      721 ttcggacacc aaaaccgctg ttgtcccgcc attctggcga ttcctgaaag ttatttgact
      781 aaaaaatagt cttaacgtgg tttattttcc gaattccaag cggaccccat aaaggccgta
      841 agatttatga atgggactcc cagcacggtg aacttgaagg gtatcgtgcc agtgatggtc
      901 agcatcttgg ctcattcgat cctaagactg gtaatcagtt gaaaggacct gatccaaaac
      961 gaaatatcaa aaagtatctt tgagaggatg ttatggggct taaattacat attaattggt
     1021 ttgataagac gaccgaggaa tttaaaggtg gtgagtattc aaaagatttc ggtgatgatg
     1081 gctcggtcat tgaacgtctt ggaatgcctt taaaagataa tatcaataat ggttggtttg
     1141 atgttatagc tgaatgggta cctttgctac aaccatactt taatcatcaa attgatattt
     1201 ccggtaatga gtattttgtt tcatttgatt atcgtgatgg tgattggtga tcaaatattc
     1261 tcaggagaga tgaaggtgga gctaaagaaa agtattagtg attataccga ggctgaattt
     1321 ctggagcttg taaaaaaaat atgtagagct gaaggtgcta ctgaagagga tgacaataaa
     1381 ttagtgagag agtttgagcg attaactgag cacccagatg gttcagatct gatttattat
     1441 cctcgcgatg acagggaaga tagtcctgaa gggattgtca aggaaattaa agaatggcga
     1501 gctgctaacg gtaagtcagg atttaaacag ggctgaaata tgaatgccgg ttgtttaagg
     1561 atgaatgact ggcattcttt cacaacaagg agtcgttatg aaaaaaataa cagggattat
     1621 tttattgctt cttgcagtca ttattctggc tgcatgtcag gcaaactata tccgggatgt
     1681 tcagggctgg actgtatccc cgtcatcaac agctgaactg accggattag caacgcagta
     1741 acccgaaatc ctctttgaca aaaacaaagc gtgtcaggct gattctgatg cgcttttttt
     1801 tgaaatgtca caaaaattcc atgtgggaga tgggatctaa aattctcgtg cagaactttc
     1861 catccagggg gagaaaactt gtcgttttga gccgttcggt gttcagaacg cacgaaaccg
     1921 atcgtgtatc gcttttgtgc atagttatgc aacgcgctaa aaacgattct gacgcatttt
     1981 ttatgtttac cctggtgttt ccttgtcttt ttgtgttttt tgcgtcagaa agcgattgag
     2041 ggtgttttaa ggggtacgta caacgggagt tatggtaaat ggatcggttt ttcgggaagg
     2101 atcgacagga tttgccgttg ggtgtagtgt aagcgactga aaaacaaacg ccccgtaaat
     2161 cgtgctctca ccgccaagat tgatcacgaa attacagggc gccgggttcc gcgtttcccg
     2221 atgggaaagc gcggttagtt aaactgtgta ccgagagaaa tcgtatcaca tgagcgccgt
     2281 acttcaacgc ttcagggaaa aattaccgca caaaccgtac tgtacgaacg atttcgcgta
     2341 cggcgttcgc attctgccga aaaacattgc cattcttgcc cgtttcatcc agcagaacca
     2401 gccacatgca ctgtactggc ttccctttga cgtggaccgg acgggggcat caatcgactg
     2461 gagcgaccgg aattgtccgg ccccgaacat caccgtaaaa aatccccgta acgggcacgc
     2521 gcatctgctc tacgcgctcg cccttcctgt gagaactgcg ccggatgcat cggcttcggc
     2581 gctcagatac gctgccgcta ttgagcgtgc gttgtgtgaa aaactgggcg cggatgtgaa
     2641 ttacagcggc ctgatctgca aaaatccgtg ccaccctgaa tggcaggaag tggaatggcg
     2701 cgaggaaccc tacactctcg acgaactggc tgattatctc gatttgagcg cctcagcgcg
     2761 ccgtagcgtc gataaaaatt acgggctggg gcgaaactgc tatctgttcg aaaagggccg
     2821 taaatgggct taccgggcta ttcgtcaggg ctggccggca ttctcacaat ggcttgatgc
     2881 ggtgattcag cgtgtcgaaa tgtacaacgc atcgctcccc gttccgcttt cacctcctga
     2941 atgtcgggct attggcaaga gtattgcgaa atacacgcac aggaacttca cgccggaaac
     3001 tttcgcacag tatgtggctg atacgcacac gccagaaatt caggctacac gcggtcgcaa
     3061 gggcggttct aagtctaagc gcggcacagt agctacatca gcacgcacgc tgaaaccttg
     3121 ggagaaatta gggatcagtc gcgcctggta ttaccaactg aaaaaacgag gtctcgtaga
     3181 gtagaccaaa taagcctata tcagataaca gcgccttttt ggcgtctttt tgagcagctt
     3241 ggtttgttgc tatttccctc gttgaatccc gcaatggtgt ggctttccgc atgattgagg
     3301 tggtagcgct cgccgcagtc tcatgtccga gcgtagcgag cgaatgagcg aggaagcgca
     3361 aaggcgtccg gtggtgcatg tggcacttac gcgccggggc ttagtggttc tgcggtttcg
     3421 ccggtggtct gggtagcttc tccagctcgt taatcagcga ttgtaatcgg ttcacatccg
     3481 cctgtcttgt gacttccttt cgcagaaact ggagcaggaa cgcacgcagt tgcgcttctt
     3541 ccggcctccg tacccttgcc agcatggctg cccccacaat gactttttgc gccgtgtcca
     3601 ggcttcggct cttcgccttc aggcgctgta atctggcctc agcttcggca atcttctgtt
     3661 cgagtgttct gctcatttcg tgactccgtg cgcggtgaaa aatcgcattt tagcgcgtca
     3721 ctggtagttg caaaactgaa ctggcataat gcacggcaca tcacgaagtg cgcacttata
     3781 caatctccac ttcgtttcga ttgtgtgcgg tctgcgacgc taaaagaaaa cggcaaaaag
     3841 gcattacggc agaaatggcg atttatcatc tcagcatgaa aatcatttcg cgaaaaaacg
     3901 gctacagtgc cgttgcttct gctgcctacc gttccggctc tgtcataccc gatgaccgta
     3961 ccggattaac ccacgattac acccgtaaac gcggcgttga tgatgcggtc attctcaccc
     4021 ctgcgaatgc accgtcctgg tgtggtgacc gttccgttct ctggaatgcg gtcgagaaag
     4081 ccgaacagcg ccggaactcc cagctggcaa gggagattga actcgccatt ccccgtgaga
     4141 tttcccgcga ggccgcacgg gagaccgttc tcgcttttgt ccgggaaaac tttgtcagtc
     4201 ggggcatgat tgccgatgtg gcgttccatc acatggaccg gaccaatccc catgcgcaca
     4261 tcatgctgac cacgagagct gtcggggaaa cgggatttgc aggaaaggtc agggactgga
     4321 acgaccgggc actcgccgag acgtggcgcg catcatgggc tgaccatgcg aacagagcgc
     4381 ttgcgaacgc cggctaccag gaagagatag accatcgttc atacgagcgt cagggactgg
     4441 agaaagcgcc cggccttcac ctcggaaagg ctgcctgtgt gatggaaaaa cggggaatgg
     4501 aaacagaacg cggtgagcag aaccgtctga ttaacagcct taacctggaa atacaggttt
     4561 cccgcacaag gctggcagtc atccaggtac aggagaacgt gcggaaacgg gaactcagcg
     4621 atgccgcacg tcgtgcagcg gaagccctta acctgaccat tcccgctgcg aatgcctcag
     4681 cggataccct gcgggaattc attgccacgt tgccgcagga atgcggcaac gcgtgggaga
     4741 tgaccccgga gttcctggcg atgagcggga aggtgaacga catcgaacgt gaggggaatg
     4801 cgctgctgaa agagcaggcc attctcgaaa aggagatgac cggactcaaa aaagcacgcc
     4861 ctgtcgcgtc ccttctgtca gagattcccc tgatgacatg ggctgaaccg gaataccgca
     4921 aaagacaact ccgtttctgg aaactcggga aacagattga atctcttcgc cgcacctaca
     4981 gggccgtgaa agaacgggac attcccgccc gtcgtcaggc ctttgaaacg cagtggaata
     5041 cgtggattgc gccgggaatg gcagagctga aagaaaaact gtcagcacgg gaagcggagc
     5101 ggcgcaggga ggaagccgaa gcggaagcgc gccggaagga acaggagcat gaggcgcggc
     5161 tgaaacgtca tgataaccac cgtctgagcc gtgaaacggc attagtcggg gttattacgg
     5221 agctggggcg tgccagagag ccgggaacgg gcaggataac ccgctacatg atgctgagta
     5281 acagagccgg agaattcacg gtatggggtg atgagctggc gcattacccc cagagtgttc
     5341 atgacccggt gaatgtttac ctgtcgccag gcggggctgt gatggtctcg gatatacgtg
     5401 agggaatgcc agaatctcat gagacgatgg cgcggcctga gcgtgtgaga atgtattccg
     5461 gtgcgacggt ccggcatgta ctggaacaga tgcgccaggg gtggccctct tacggttttc
     5521 cggcgctgcc gcatcactgg ccggataatt tttatttcag cgacgaccgc aggcccgtag
     5581 cctctccgct gccgtcagcg caccgggtgg atatcaccgc tgatgctgta ccggaacaac
     5641 tcatgcctgt tgtattttcg agagagggaa acagcaggac gctgaatctg ttgttatgca
     5701 aagggccgga ggaaatgctt gtcgggtttg ttcgccggga aggagggttt tgccccgttc
     5761 ttgtgcttcc gtcgccggat tacagtcatc tgatagtcag caccatcacg gagaacgggg
     5821 tatgcctggc aggttacgga gaagctataa accatgatga ggatactccg tacccaccgg
     5881 aaccgcacct gatgcagttc cggctcaaag gccatcatga caggcttttg gctgctgtcc
     5941 acaaaccgga agagatgccg gattatctct tccgtcaact cggttttaat cagacctggc
     6001 atgagtggaa gcgggacgaa cagcacaggc aacaacaacg ccgccccggg cacttccggg
     6061 gcatgagtat gtgatgtccg gggctgcacc ccggaccccg ccaacacatc acgggccaca
     6121 aaattttttg tggcccgctc tgcgttttct aagtgttatc cctcctgatt tctaaaaaat
     6181 tttccacctg agcttgacag aaaaaacgat gacgggtact ttttgatctg tacataaaac
     6241 cagtggtttt atgtacagta ttaatcatgt aattaattgt tttaacgctt aaaagaggga
     6301 atttttatga gcggtggcga tggacgcggc cataacacgg gcgcgcatag cacaagtggt
     6361 aacattaatg gtggcccgac cgggcttggt gtaggtggtg gtgcttctga tggttccggg
     6421 tggagttcgg aaaataaccc gtggggtggt ggttccggta gcggcattca ctggggaggt
     6481 ggttccggtc atggtaatgg cggggggaat ggtaattccg gtggtggctc gggaacaggc
     6541 ggtaatctgt cagcagtagc tgcgccagtg gcatttggtt ttccggcact ttccactcca
     6601 ggagctggcg gtctggcggt cagtatttca gcgggagcat tatcggcagc tattgctgat
     6661 attatggctg ccctgaaagg accgtttaaa tttggtcttt ggggggtggc tttatatggt
     6721 gtattgccat cacaaatagc gaaagatgac cccaatatga tgtcaaagat tgtgacgtca
     6781 ttacccgcag atgatattac tgaatcacct gtcagttcat tacctctcga taaggcaaca
     6841 gtaaacgtaa atgttcgtgt tgttgatgat gtaaaagacg aacgacagaa tatttcggtt
     6901 gtttcaggtg ttccgatgag tgttccggtg gttgatgcaa aacctaccga acgtccgggt
     6961 gtttttacgg catcaattcc aggtgcacct gttctgaata tttcagttaa taacagtacg
     7021 ccagcagtac agacattaag cccaggtgtt acaaataata ctgataagga tgttcgcccg
     7081 gcaggattta ctcagggtgg taataccagg gatgcagtta ttcgattccc gaaggacagc
     7141 ggtcataatg ccgtatatgt ttcagtgagt gatgttctta gccctgacca ggtaaaacaa
     7201 cgtcaggatg aagaaaatcg ccgtcagcag gaatgggatg ctacgcatcc ggttgaagcg
     7261 gctgagcgaa attatgaacg cgcgcgtgca gagctgaatc aggcaaatga agatgttgcc
     7321 agaaatcagg agcgacaggc taaagctgtt caggtttata attcgcgtaa aagcgaactt
     7381 gatgcagcga ataagactct tgctgatgca atagctgaaa taaaacaatt tgatcgattt
     7441 gcccatgacc caatgtctgg cggtcacaga atgtggcaaa tggccgggct taaagcccag
     7501 cgggcgcaga cggatgtaaa taataagcag gctgcatttg atgctgctgc aaaagagaag
     7561 tcagatgctg atgctgcatt aagtgccgcg caggagcgcc gcaaacagaa ggaaaataaa
     7621 gaaaaggacg ctaaggataa attagataag gagagtaaac ggaataagcc agggaaggcg
     7681 acaggtaaag gtaaaccagt tggtgataaa tggctggatg atgcaggtaa agattcagga
     7741 gcgccaattc cagatcgcat tgctgataag ttgcgtgata aagaatttaa aaacttcgac
     7801 gattttcgga gaaaattctg ggaagaagtg tcgaaagatc ctgagttaag taagcaattt
     7861 aatccaggta ataaaaaacg cttgagccaa ggattagctc cacgggctag gaataaagat
     7921 actgtcggtg ggcgtcgctc atttgagctt catcacgaca agccaatcag tcaggatggt
     7981 ggtgtttatg atatggacaa cctccgtatc actacgccga aacgccatat tgatattcac
     8041 agaggtcagt aaaaatggaa ctgaaaaaca gcattagtga ttacactgaa actgaattca
     8101 aaaaaattat tgaagacatc atcaattgtg aaggtgatg
//
LOCUS       DAZGAN020000168         8117 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGAN020000168 DAZGAN020000000
VERSION     DAZGAN020000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.168"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..259
                     /locus_tag="ACHKYO_RS23025"
                     /old_locus_tag="pgaptmp_004605"
     CDS             <1..259
                     /locus_tag="ACHKYO_RS23025"
                     /old_locus_tag="pgaptmp_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431313.1"
                     /translation="NIFILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRH
                     EILPVNLTPETLFGSKSQDKHALFPIFTASWRATRIKNKGI"
     gene            482..979
                     /gene="ubiC"
                     /locus_tag="ACHKYO_RS23030"
                     /old_locus_tag="pgaptmp_004606"
     CDS             482..979
                     /gene="ubiC"
                     /locus_tag="ACHKYO_RS23030"
                     /old_locus_tag="pgaptmp_004606"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HGU4431314.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            992..1864
                     /gene="ubiA"
                     /locus_tag="ACHKYO_RS23035"
                     /old_locus_tag="pgaptmp_004607"
     CDS             992..1864
                     /gene="ubiA"
                     /locus_tag="ACHKYO_RS23035"
                     /old_locus_tag="pgaptmp_004607"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HGU4431315.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2019..4442)
                     /gene="plsB"
                     /locus_tag="ACHKYO_RS23040"
                     /old_locus_tag="pgaptmp_004608"
     CDS             complement(2019..4442)
                     /gene="plsB"
                     /locus_tag="ACHKYO_RS23040"
                     /old_locus_tag="pgaptmp_004608"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HGU4431316.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4613..4981
                     /gene="dgkA"
                     /locus_tag="ACHKYO_RS23045"
                     /old_locus_tag="pgaptmp_004609"
     CDS             4613..4981
                     /gene="dgkA"
                     /locus_tag="ACHKYO_RS23045"
                     /old_locus_tag="pgaptmp_004609"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGU4431317.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5091..5699
                     /gene="lexA"
                     /locus_tag="ACHKYO_RS23050"
                     /old_locus_tag="pgaptmp_004610"
     CDS             5091..5699
                     /gene="lexA"
                     /locus_tag="ACHKYO_RS23050"
                     /old_locus_tag="pgaptmp_004610"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HGU4431318.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5772..7097
                     /gene="dinF"
                     /locus_tag="ACHKYO_RS23055"
                     /old_locus_tag="pgaptmp_004611"
     CDS             5772..7097
                     /gene="dinF"
                     /locus_tag="ACHKYO_RS23055"
                     /old_locus_tag="pgaptmp_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HGU4431319.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7213..7422
                     /gene="yjbJ"
                     /locus_tag="ACHKYO_RS23060"
                     /old_locus_tag="pgaptmp_004612"
     CDS             7213..7422
                     /gene="yjbJ"
                     /locus_tag="ACHKYO_RS23060"
                     /old_locus_tag="pgaptmp_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGU4431320.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7464..7979)
                     /gene="zur"
                     /locus_tag="ACHKYO_RS23065"
                     /old_locus_tag="pgaptmp_004613"
     CDS             complement(7464..7979)
                     /gene="zur"
                     /locus_tag="ACHKYO_RS23065"
                     /old_locus_tag="pgaptmp_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HGU4431321.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1815 a         1989 c         2232 g         2081 t
ORIGIN      
        1 taatatattt atcctgttct cctctgtcga tgatgggcct gttatggtgg taagctccca
       61 gcgcttacat gatatgttga atcctacaaa agataccaat tggaattcca cgtatatcta
      121 caaatccaga catgagatat tgcctgttaa tcttactccg gaaacacttt tcggctccaa
      181 atctcaggat aaacatgcac ttttccccat ttttactgcg agttggcgag ctactcgtat
      241 aaagaataaa ggtatttaag taaaggaaaa catcaccatt cctggcatca tgggcggtga
      301 tgcccctatg gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt caacagcgtt
      361 ttaccacttc atgcgattgt tgcgtttttg ttgcgcatta gatcacttaa tttgctttac
      421 atctcccgta aacacttttc tgcgatacaa tgcctttacg ttatgtaacg gagagttcgg
      481 catgtcacac cccgcgttaa cgcaactgcg tgcgctgcgc tattttaaag agatccctgc
      541 cctggagccg caactgctcg actggctgtt gctggaagat tccatgacaa aacgttttga
      601 acagcaggga aaaacggtga gcgtgacgat gatccgcgaa gggtttgtcg agcagaatga
      661 aattcccgaa gaactgccgc tgctgccgaa agagtctcgt tactggttac gtgaaatttt
      721 gttatgtgcc gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg tgtcaacgtt
      781 aagcgggccg gagctggcgt tacaaaaatt gggtaaaacg ccgttaggac gctatctgtt
      841 cacatcatcg acattaaccc gggactttat tgagataggc cgtgatgccg gactgtgggg
      901 gcgacgttcc cgcctgcgat taagcggtaa accgctgttg ctaacagaac tgtttttacc
      961 ggcgtcaccg ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc agaataagct
     1021 gctggcgttt catcgcttaa tgcgtacgga taagccaatt ggcgcgttac tgctgctctg
     1081 gccaacatta tgggcgttgt gggtggcgac accgggcgtt cctcagctct ggatcctggc
     1141 ggtgtttgtc gcgggtgtct ggctgatgcg cgctgccgga tgtgtggtga atgattatgc
     1201 tgaccgcaag tttgatggtc atgttaagcg cacggcgaac cgaccgcttc ccagcggcgc
     1261 ggtaacggag aaagaggcgc gcgcgctgtt tgtagtgctg gtattgattt catttttact
     1321 ggtgctgacg ctgaatacga tgactattct gttgtcgatt gccgcgctgg cgctggcgtg
     1381 ggtataccca tttatgaagc ggtataccca tctaccgcaa gtggtgctgg gcgcggcgtt
     1441 tggctggtcg attccaatgg cttttgccgc tgtgagtgag tcggtgccat tgagttgctg
     1501 gttaatgttc ctcgccaata ttctctgggc ggtggcttac gacacgcagt atgcgatggt
     1561 tgaccgcgat gatgatgtga agattggcat taaatccacg gcaatcctgt tcggccaata
     1621 cgataaattg attattggta ttttgcagat tggcgtactg gcactgatgg cgatcatcgg
     1681 tgagttaaat ggcttaggct ggggatatta ctggtcaatt gtggtggctg gcgcgctgtt
     1741 tgtttatcaa caaaaactga ttgccaaccg cgagcgtgaa gcctgcttta aagcatttat
     1801 gaataataac tatgttggtc tggtactatt tttagggctg gcaatgagtt actggcattt
     1861 ctgatgatgt aaaaaagccg gatgatcatc cggctttctt ctgggttgcc tgatgcgcgg
     1921 cgcttctcag gcctacacaa cacatcgcaa tttattgaat ttgcagatta tggaaggccg
     1981 gataaggcgt tttcgccgca tccggcaatt ctctctgatt acccttcgcc ctgcgtcgca
     2041 ctctcaatcg tcaaacgcac gtctgatgta atcaactccg ccagcaactg ataaaccttc
     2101 atcgtttctg ccggttcggc atcgccgcta tcgctgatat acccttcatc acgcagtgtc
     2161 agcaccagag aactgaacac cgccttgtcg aagaactccg gcgcgttgat gccgtgcagc
     2221 acggagagac gttgcgcgac ggtgcggctc tctttctcca gcgtaccgcg gttgatcgac
     2281 gggttggcac tcaacaacca gaaggtgatg gcataacgtt gcagcgtttc gcgcgcgcct
     2341 gcggcgagca gctgtagcgt gcgagaatgc accgggttga tatgcaactc atcatcttgc
     2401 agggtaatca gcccctgacg ttgcatctca tttgccagcg catcaataac gtccggcaac
     2461 tcgtcgcgat cccagcgcag gaacagctcc gctttcagca ttgggtaaag cacattgacg
     2521 tgctccatca atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc cgccatcagc
     2581 gaaggcagca ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag caccgcttgc
     2641 tcgcgtggca gaatgatgat gtcgccgata gtgtctttct cgacttcaaa cttgttcatt
     2701 tgcagcgcgt gatcgataag ctcgctggcg ctggctgaag gaacggtaga gtccgtggag
     2761 taaggtacgt tgcgcatcag atccaggtag cagttgagtt gctcggttaa ctgctcgcgg
     2821 gtgagtgagc gctgacgtga tgccagtagc gcagtacagc acaggttcat ggcgtttgcc
     2881 gcgcctgcgt tgttaatgcg taccatcaga tcggcagcaa tattattgac cgtcggcgtt
     2941 aaccaggccg gacgcaccgc ttcgatggga tcgatagatt cacgccagtc tggtacgtgc
     3001 tggttaaggt aggtcatcaa cggcattggt tcaccgaagt tgacgtaacc ctgaccgaga
     3061 ttacgcagct tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc tttcgtcgcg
     3121 ccgcgcagtt ctttggcgta agtacccact tccatgacgt gctcataacc gatatagatc
     3181 ggaatcagcg taatcggacg cgtgccgcca cgcagcatcg cctgaatggt catcgacagc
     3241 gtaccggttt tcggatccag caaacgcccc gtgcgggaac gaccgccttc cacgaagtac
     3301 tcgacggaat aaccacggct gaacagttcg ccgagatact cacggaaaac ggtggaataa
     3361 agtttattgc ctttaaaagt acggcgaata aagaacgccc ccagacggcg gaaaatcggc
     3421 ccggccggcc agaagttcag gttgatcccg gcggcgatat gcggcggcac cagcccctgg
     3481 tggtacagca cgtaagaaag tagcaggtag tccatgtgac tgcggtggca aggcacatac
     3541 accagctcat gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg gacgttgatg
     3601 ccctgataaa gtcggttcca ggtgaagccc agaatgcggt cagtcaggcg gatcatctcg
     3661 taagagaaat tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg cgctttttca
     3721 tgggagattt ttttgctgcg cgcttcatct tctaccgctt tggcaatggc gcgggaggcg
     3781 agcagcttat taaacagatc ctgacgagca ggcagacgtg ggcccacagc agccagacgt
     3841 tgacgggcaa agtgcatacg cgccacgcgc gccagtttct gtgcgatagt tttatccgtg
     3901 ccgtgttcat ccgccatacg gcgcagcgaa actgacggcg agaaacgcac aaaactgtcg
     3961 cgaccgagcc acagtacggc gaaaaatttc tgtacgccgt taagcatacg cagcggcggg
     4021 tttacttcgc ctttttcacg ccccggcgcg cgaccaaaca tcaccgatac tggcaccatc
     4081 tgcacatcca gatttgggtt gctacggtgc aaatcgagat agtcgtggaa cagcttaata
     4141 gactcttctt tcggcgtgta ataggtgaat acacgcggcc cgccgtgaat gaacacatag
     4201 cgcggcagta gcgtgccgtc gatttccagc ggctctaacg ggtcaggcaa gtcatgagcc
     4261 agacactggg cgcgtaacgt cagcaaatct gctttcgagt tgtacggtaa aacgtacata
     4321 attggacgag aagtatccag ccccagttcc ggggcaggat ctgccggaat agacttgctt
     4381 tttaccagga tgcttaatgg taaattcagt aatttgtagt aaattcgtgg ccagccggac
     4441 ataaacgatg taaagcctct ggttaataat gcaaatgcgc ggcaaggata gcagaaagtc
     4501 atgggaaatt ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac ccgaatcatc
     4561 ggatttaacg gtacactgat attgacgctc ataatgtaaa aaggttcttt caatggccaa
     4621 taataccact ggattcaccc gaattatcaa agctgctggc tattcctgga aaggtttacg
     4681 cgctgcatgg atcaacgaag cggcattccg tcaggaaggc gtagcggtat tgttggcggt
     4741 ggtcatcgcc tgctggctgg atgtggacgc aattacccgc gtgctgctta tcagctccgt
     4801 gatgctggtg atgattgtgg aaattctcaa tagcgccatc gaagcagtgg ttgaccgaat
     4861 tggctctgaa taccatgagc tttccggacg cgctaaagat atgggatccg ccgcggtgct
     4921 gattgccatt atcgtcgccg tgattacctg gtgcattctg ttatggtcgc attttggata
     4981 acccttccag atttcgataa atctctggtt tattgtgcag tttatggttc caaaatcgcc
     5041 ttttgctgta tatactcaca gcataactgt atatacaccc agggggcgga atgaaagcgt
     5101 taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta
     5161 tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg
     5221 aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac
     5281 gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg
     5341 ccggtgaacc gcttctggcg caacagcata ttgaaggtca ttatcaggtc gatccttcct
     5401 tgttcaagcc gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg
     5461 gcattatgga tggtgacttg ctggcagtgc ataaaactca ggatgtacgt aacggtcagg
     5521 tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa cagggcaata
     5581 aagtcgaact gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac cttcgtcagc
     5641 agagcttcac cattgaaggg ctggcggttg gggttattcg caacggcgac tggctgtaac
     5701 atatctctga gaccgcgatg ccgccaggcg tcgcggtttg tttttcatct ctcttcatca
     5761 ggcttgtctg catggcattc ctcacttcat ctgataaagc actctggcat ctcgccttac
     5821 ccatgatttt ctccaatatc accgttccgt tgctgggact ggtcgatacg gcggtaattg
     5881 gtcatcttga tagcccggtt tatttgggcg gcgtggcggt tggtgcaacg gcgaccagct
     5941 ttctctttat gctgttgctg tttttacgca tgagcaccac cgggctgact gcgcaggctt
     6001 atggtgccaa aaatcctcag gcattagccc gtgcgctggt gcaaccgttg ctgttggcgt
     6061 tgggggctgg ggcgttaatt gcgctgctgc gtacaccgat tatcgatctg gcgctgcata
     6121 ttgttggcgg tagtgaagcg gtcctggaac aggcgcggcg ctttcttgaa atccgctggt
     6181 taagcgctcc ggcgtcgctg gcgaatctgg tattactcgg ttggttactc ggcgtgcaat
     6241 atgcccgtgc gccagtaatt ttgttagtgg tcggcaatat cctcaacatt gtgctggatg
     6301 tctggctggt gatggggctg catatgaacg tgcagggcgc ggcgctggcg acggttattg
     6361 cggaatatgc aacattgctg attggtctgc taatggtgcg taaaatcctc aaactacgcg
     6421 gaatttccgg cgaaatgctg aaaactgcct ggcgaggaaa cttccgtcgc ttgctggcgc
     6481 ttaaccgcga tatcatgctg cgttcgctgt tgttgcaact ctgtttcggc gcgatcaccg
     6541 tacttggtgc gcgactgggg agtgacattg tcgctgttaa cgcggttctg atgacgctac
     6601 tcacctttac cgcctatgcg cttgatggtt ttgcctacgc ggttgaagcg cactccggtc
     6661 aggcatacgg tgcgcgcgac ggtagccagt tgctggatgt atggcgggca gcgtgccgcc
     6721 agtcggggat tgtagcgtta ctgttttcgg tggtttattt gctggcaggg gaacacatca
     6781 ttgcgttgct gacgtcgtta acccagattc agcagctggc tgaccgctat cttatctggc
     6841 aggtgatttt gccgttggtt ggcgtctggt gttatctgct ggacggcatg tttataggcg
     6901 caacgcgtgc cgccgaaatg cgtaacagta tggcggtggc cgccgcaggt tttgcgctga
     6961 cgctccttac gctgccgtgg ctgggtaatc atggtttgtg gctggcatta accgtctttc
     7021 tggcgttgcg cgggctttct ctggcggcta tctggcggcg tcactggcgc aacggtacct
     7081 ggtttgccgc aacgtgacgg ttaaaaattc tgaataaatt atcctaagcc aaattgctga
     7141 ctacacttaa tctcacgttc agaagaaaag tgaacgtact ctcattcaca acctaacgat
     7201 gaggtcttga ttatgaataa agatgaagcc ggcggtaact ggaaacagtt taaaggtaaa
     7261 gtgaaagagc aatggggcaa actgaccgat gatgatatga cgatcattga aggtaaacgt
     7321 gatcaactgg tcggtaaaat ccaggaacgt tatggttatc agaaagatca ggcagaaaaa
     7381 gaggtcgtgg attgggaaac ccgcaatgaa tatcgctggt aattaatccc tcctgcccga
     7441 cgtgtacaag gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac agagtgatca
     7501 tgctggcact gttcaggatg acgacacgct tccacttcca cgcatgccgc acagagccca
     7561 tgtgcttcaa tcacattatg ccgcagggca aaccccattt ttgccgccag cgtatgcata
     7621 atgtcttcca cgccttctgc acactcttct ttcaccgcgc cgcagcgatc gcatataaac
     7681 atggctgacg tatgagtggg ctggtcgaac agatgacaga gcacataact gttggtggat
     7741 tccaccttat gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata aaccgttggc
     7801 ggcttggctt gcggttcagc ttcgcgcagt aaatcaagca gatcataagc gctgatagcg
     7861 ccatcctgca gactcatcag gcgcaacact tccaggcgct gtggggtcag gcgcacatta
     7921 cgctgcgcgc agattttttc agcctgcgct aataactcct gcgttgtggt cttttccatc
     7981 gggcacctca aagtgggggt taagaatgtc cttactttac catgttccag gaaaaacacc
     8041 gagaaccctt attgttgccg taatgttgat tttctgtttt gtaggtaagg tgttgtgttg
     8101 ccttgtcgta ccattat
//
LOCUS       DAZGAN020000169         8065 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGAN020000169 DAZGAN020000000
VERSION     DAZGAN020000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.169"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(54..1217)
                     /gene="fadA"
                     /locus_tag="ACHKYO_RS23070"
                     /old_locus_tag="pgaptmp_004614"
     CDS             complement(54..1217)
                     /gene="fadA"
                     /locus_tag="ACHKYO_RS23070"
                     /old_locus_tag="pgaptmp_004614"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HGU4431322.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(1227..3416)
                     /gene="fadB"
                     /locus_tag="ACHKYO_RS23075"
                     /old_locus_tag="pgaptmp_004615"
     CDS             complement(1227..3416)
                     /gene="fadB"
                     /locus_tag="ACHKYO_RS23075"
                     /old_locus_tag="pgaptmp_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HGU4431323.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPNTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSLLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDTIDALFDANRFGQKNGLGFWRYKEGSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEESIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            3606..4937
                     /gene="pepQ"
                     /locus_tag="ACHKYO_RS23080"
                     /old_locus_tag="pgaptmp_004616"
     CDS             3606..4937
                     /gene="pepQ"
                     /locus_tag="ACHKYO_RS23080"
                     /old_locus_tag="pgaptmp_004616"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HGU4431324.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4937..5551
                     /locus_tag="ACHKYO_RS23085"
                     /old_locus_tag="pgaptmp_004617"
     CDS             4937..5551
                     /locus_tag="ACHKYO_RS23085"
                     /old_locus_tag="pgaptmp_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HGU4431325.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            5590..7041
                     /gene="trkH"
                     /locus_tag="ACHKYO_RS23090"
                     /old_locus_tag="pgaptmp_004618"
     CDS             5590..7041
                     /gene="trkH"
                     /locus_tag="ACHKYO_RS23090"
                     /old_locus_tag="pgaptmp_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HGU4431326.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            7053..7598
                     /gene="hemG"
                     /locus_tag="ACHKYO_RS23095"
                     /old_locus_tag="pgaptmp_004619"
     CDS             7053..7598
                     /gene="hemG"
                     /locus_tag="ACHKYO_RS23095"
                     /old_locus_tag="pgaptmp_004619"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HGU4431327.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
     gene            7974..>8065
                     /locus_tag="ACHKYO_RS23100"
                     /old_locus_tag="pgaptmp_004620"
     rRNA            7974..>8065
                     /locus_tag="ACHKYO_RS23100"
                     /old_locus_tag="pgaptmp_004620"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1817 a         2159 c         2185 g         1904 t
ORIGIN      
        1 tggtgccgga tcggtagacc ggataaggcg ttcacgccgc atccggcaag tggttaaacc
       61 cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc cagaccaaac
      121 tggacgtctt tgcgctccat cagatttagc agcgtggtgc tgatacgcgc accggaacag
      181 cccagcggat gacccagcgc gatcgcgcca ccgttgaggt tgatcttctc gtcaatctgc
      241 tccattagcc ccagatcttt aatacatggc aggatctgcg cggcaaaggc ttcattcatt
      301 tcaaacacgc cgatatcact gacagaaagc cccgcttttt tcagcgccag cttcgaggcc
      361 ggaaccgggc cgtaacccat aatcgatggg tcacaaccaa cgaccgccat cgaacgcacg
      421 cgggcgcgcg gcttaagacc taattcatgg gcgcggcttt cactcatcac cagcatggca
      481 gctgcgccat cggaaagcgc agaagatgta cccgccgtta ccgtaccgct tactggatca
      541 aacgccggac gcagcgtggc gagggcttcc acggtggttt ccgggcgaat cacttcgtcg
      601 taattaaact gcttcaggac gccgtcggca tcgtgaccac cggtcgggat aatttcattt
      661 ttaaatgcac ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca
      721 tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgccgt taagcccatc
      781 atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcgac gccgtggctc
      841 attggcacat ggcccatatg ctccacgccg ccgacaagac atgcctgcgc atcgccagtc
      901 atgatcattc gtgctgcgtc atgcaatgcc tgcatggatg aaccacacaa gcgattgacg
      961 gtcaccgccg ggacagagtg tggtacttct gccagcagcg ccgcgttacg ggcgatatta
     1021 aaaccctgct ccagcgtctg ctgcacacaa ccccagtaaa tatcgtcgag ggccgccgct
     1081 tccagcgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag atcttctgca
     1141 cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca
     1201 atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac cggacgggct
     1261 ggctcaaccg ggggatagta cggttcgtta tgacgcgctt tattacgcag accttccggc
     1321 acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta
     1381 ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc caggccgtag
     1441 accagcgcca tatccgcttc tgccggagtg gcgataatgc tttcttccag acagcgcacc
     1501 acttcgttga ccatcgggat catcatgcgg gcgataatct cttcttcgct gaaatcgcgc
     1561 ttcggctggc tcacttctgc cagcaggtcg tcaacgacgg cgtcttcttc tttcttcggc
     1621 ttacctttgc tgccttcttt ataacgccag aaaccgaggc cgttcttctg accaaagcgg
     1681 ttggcatcaa acagcgcgtc gatggtatcg cgataatctt tctgcatccg ctgcgggaag
     1741 cctgctgcca tgacagcctg ggcatgatgc gcggtgtcaa tgcccacgac atccagcaga
     1801 tatgccggac ccatcggcca gccaaactgt ttttccatta ctttgtcgat cttgcggaaa
     1861 tccgcgccgt cgcgcagcaa caggctgaaa ccggcgaaat acgggaacag cacgcggttg
     1921 acgaagaagc cggggcagtc gttaaccaca atcggcgttt tgcccatctt gctcgcccag
     1981 gcgacgactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaattat ttctaccaac
     2041 ggcattcggt ggaccgggtt aaagaagtgc atcccgcaga agttttctgg acgttccagc
     2101 gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagcac ggtattcggg
     2161 cgtactttct gctcggtttc tgccagtacg gcttttttca cttttgggtt ttcgacaacc
     2221 gcttctacca caacatccac gcggtcaaac ccggcgtagt cgagcgttgg gtggattgtg
     2281 gagatcacgc cagccagttt cagaccatcg atcttgccgc gctcaagctg cttgttcagc
     2341 agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc tttcatgaca
     2401 accggcacgc ctttccacgc agactggtaa gcgatgccgc cgcccataat gcctgcaccc
     2461 agcaccgcgg cctgtttcgg ggtttcaacg tctttggtga gtttcttcgc tttgcctttt
     2521 acatattgat cgttaaggaa aatgccgacc agtgcgcggg cttcgttggt atgcgccagc
     2581 gggacaaaac ttttgttttc caggtttaag gcttcttcac gaccaaaacg ggccgcagct
     2641 tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgctgt ttgtgcgacc
     2701 atccctttag cgatggtgaa gctcatggtg gcttcaatct tgctcagttt tagtggttcc
     2761 agcttcggct gacgttttgc tttccagtcg aggtcgccgt taatggcctg gcgtaaaacc
     2821 gcctttgcgc cttcaaccag tttttctgct ttgactacgc catccaccag accgattttc
     2881 agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagcgc actgtcagcg
     2941 cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt
     3001 tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc cagcacgcat
     3061 tcgcagccac cgcccagcgc atagccgttg acggcagcaa tggtcggcac cggcagatct
     3121 tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg ttcttcagga
     3181 acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc tgctttgttc
     3241 gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat ggcctcgccg
     3301 aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc aaataccagt
     3361 tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta aagcatgtca
     3421 gtctcctgaa tccgcaaggt gatctggtcg taccagatgc atcgaagtgt gtattttgtg
     3481 ttaaaaatat gcaaataaaa gattaaagaa atgccgatct gatcacgctc ggcagaaatc
     3541 acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt
     3601 aaaaaatgga atcactggcc tcactctata aaaatcatat agctacctta caggaacgga
     3661 ctcgcgatgc gctggcgcgc ttcaagctgg atgcgttact tattcactcc ggcgaactgt
     3721 tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt
     3781 gggtgccggt aactcaggtg ccaaactgct ggctgctggt ggatggcgtg aacaagccga
     3841 aactgtggtt ctatctgccg gttgattact ggcacaacgt cgaaccgctg ccgacctctt
     3901 tctggactga agacgtggaa gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc
     3961 tgcctgctgc gcgcgggaat atcggttata tcggtccggt gccggaacgt gcgctgcaac
     4021 tgggtattga ggccagcaat atcaacccga aaggggtgat cgactacctg cattactacc
     4081 gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa atggcggtca
     4141 acggtcatcg cgcggcagaa gaagcgttcc gttctggcat gagcgagttc gatatcaaca
     4201 ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttacagc aacattgtgg
     4261 cgctcaacga acacgctgcg gtgttgcatt acaccaaact ggaccaccag gcaccggaag
     4321 agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcg gctgacctga
     4381 cccgtacctg gtcggcaaaa agtgacaacg actacgcaca gctggtgaaa gacgtaaatg
     4441 atgaacaact ggcgctgatc gctaccatga aagcaggcgt cagctatgtg gattaccaca
     4501 tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc accgatatga
     4561 gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac ggtattggcc
     4621 atccgctggg cctgcaggtg catgacgtag ccggttttat gcaggatgat agcggtacgc
     4681 acctcgcggc accggcaaaa tatccgtacc tgcgctgcac ccgtattctc cagccgggca
     4741 tggtgttaac catcgaaccg ggtatctact tcattgaatc gctgctggca ccgtggcgtg
     4801 aagggcagtt cagcaagcac ttcaactggc agaaaattga agcactgaaa ccgttcggcg
     4861 gcattcgtat cgaagacaac gtggtgatcc acgaaaataa cgtggaaaac atgacccggg
     4921 atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca cggtcgttga
     4981 agagatcaaa aagagccgct tcataacgat gttggcgcat accgatggcg ttgaggcggc
     5041 gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccgccacc attgcgtggc
     5101 gtgggtggcg ggtgcgccgg atgattcaca acagctgggt ttctctgacg acggggagcc
     5161 ggcgggaacg gcaggtaaac cgatgctcgc ccagctaatg ggcagcggcg ttggggaaat
     5221 taccgctgtg gtggtgcgct actacggcgg catattgctt ggcaccggtg ggttagtgaa
     5281 agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac gcaagacgcc
     5341 attaaccgaa tatactttgc aatgtgaata tcatcagtta accggcattg aagcgttgct
     5401 ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc tgctgcgggt
     5461 ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg
     5521 ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag aactaaggaa
     5581 gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt catcttattt
     5641 tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg agcgggccgc
     5701 gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctgtg gtggccgaac
     5761 cgcaaagaga aaggcgaact taaatcccgt gaggggtttc tgatagtggt gctgttctgg
     5821 accgtgctgg gtagcgtcgg tgcgctccct tttatcttct cggaaagccc gaacctcacg
     5881 attaccgatg cgttttttga atctttctct ggcctgacca ccaccggggc cactacgctg
     5941 gtggggctgg attcgctccc tcacgccatc ctcttttatc gccagatgct gcaatggttt
     6001 ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg
     6061 atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga
     6121 attgcggaaa cggcgaaaac cctgtggttg atttatgtct tgctgaccgt cgcctgtgcg
     6181 ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag ctttgcgact
     6241 atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga tagcccgact
     6301 attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg tctgcacttt
     6361 tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt tcgcatgttt
     6421 atcggcgtac agtttacgct ggtggttatt tgtaccctcg tactgtggtt tcataatgtc
     6481 tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtgtc gatggcgaca
     6541 accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc ggtactgctt
     6601 ttatgttcgg cttttatcgg cggttgtgcc gggtcaacgg gcggtggcct gaaagtgatc
     6661 cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga aacgactggt gcatccgaac
     6721 gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt
     6781 tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc gattatcgcc
     6841 acgggcgtgg atgacttttc tgcctttgcc tcagttgttg cgacattgaa taacctgggg
     6901 ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc taaatggatc
     6961 ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattgctggt gctctttacc
     7021 ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt ttctcaacaa
     7081 gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa gaactgggga
     7141 tccagacgga tgtcgccaat gtgcaccgca ttgaagaacc acaatgggaa aattatgacc
     7201 gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc caggaatttg
     7261 tcaaaaaaca tgcgacgcga ctgaattcga tgccgagcgc cttttattcc gtaaacctgg
     7321 tggcgcgcaa accggagaag cgtactccac agaccaacag ctacgcgcgg aagtttctga
     7381 tgaactcgca atggcgtccc gatcgctgcg cggtcattgc cggggcgctg cgttacccac
     7441 gttatcgctg gtacgaccgt tttatgatca agctgattat gaagatgtca ggcggtgaaa
     7501 cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat ttcgcccgag
     7561 aaatcgccca tttaaccgac aaaccgacgc tgaaataagc acaaagaata aaaaatgcgc
     7621 ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
     7681 accactgaca cggaacaacg gcaaacacgt tgccgggtca gcggggttct cctgagaatc
     7741 tcaacagaga aaagcaaaaa atgcttgact ctgtagcggg aaagcgtatt atgcacaccc
     7801 cgcgccgctg agaaaaagcg aagcggcact gctctttaac aatttatcag acaatctgtg
     7861 tgggcactcg aagatacgga ttcttaacgt cacaagacga aaaatgaata ccaagtctca
     7921 agagtgaaca cgtaattcat tacgaagttt aattctttga gcatcaaact tttaaattga
     7981 agagtttgat catggctcag attgaacgct ggcggcaggc ctaacacatg caagtcgaac
     8041 ggtaacagga agcagcttgc tgctt
//
LOCUS       DAZGAN020000170         7980 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGAN020000170 DAZGAN020000000
VERSION     DAZGAN020000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.170"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(171..314)
                     /gene="ypaB"
                     /locus_tag="ACHKYO_RS23105"
                     /old_locus_tag="pgaptmp_004621"
     CDS             complement(171..314)
                     /gene="ypaB"
                     /locus_tag="ACHKYO_RS23105"
                     /old_locus_tag="pgaptmp_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HGU4431328.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            442..2727
                     /gene="nrdA"
                     /locus_tag="ACHKYO_RS23110"
                     /old_locus_tag="pgaptmp_004622"
     CDS             442..2727
                     /gene="nrdA"
                     /locus_tag="ACHKYO_RS23110"
                     /old_locus_tag="pgaptmp_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU4431329.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2874..4004
                     /gene="nrdB"
                     /locus_tag="ACHKYO_RS23115"
                     /old_locus_tag="pgaptmp_004623"
     CDS             2874..4004
                     /gene="nrdB"
                     /locus_tag="ACHKYO_RS23115"
                     /old_locus_tag="pgaptmp_004623"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU4431330.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4004..4258
                     /gene="yfaE"
                     /locus_tag="ACHKYO_RS23120"
                     /old_locus_tag="pgaptmp_004624"
     CDS             4004..4258
                     /gene="yfaE"
                     /locus_tag="ACHKYO_RS23120"
                     /old_locus_tag="pgaptmp_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HGU4431331.1"
                     /translation="MARVTLRITGTQLLCQNEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4312..4962)
                     /gene="inaA"
                     /locus_tag="ACHKYO_RS23125"
                     /old_locus_tag="pgaptmp_004625"
     CDS             complement(4312..4962)
                     /gene="inaA"
                     /locus_tag="ACHKYO_RS23125"
                     /old_locus_tag="pgaptmp_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HGU4431332.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5042..5146)
                     /locus_tag="ACHKYO_RS23130"
                     /old_locus_tag="pgaptmp_004626"
                     /pseudo
     CDS             complement(5042..5146)
                     /locus_tag="ACHKYO_RS23130"
                     /old_locus_tag="pgaptmp_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5144..5383
                     /locus_tag="ACHKYO_RS23135"
                     /old_locus_tag="pgaptmp_004627"
                     /pseudo
     CDS             5144..5383
                     /locus_tag="ACHKYO_RS23135"
                     /old_locus_tag="pgaptmp_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5425..6501)
                     /gene="glpQ"
                     /locus_tag="ACHKYO_RS23140"
                     /old_locus_tag="pgaptmp_004628"
     CDS             complement(5425..6501)
                     /gene="glpQ"
                     /locus_tag="ACHKYO_RS23140"
                     /old_locus_tag="pgaptmp_004628"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU4431333.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6506..7864)
                     /gene="glpT"
                     /locus_tag="ACHKYO_RS23145"
                     /old_locus_tag="pgaptmp_004629"
     CDS             complement(6506..7864)
                     /gene="glpT"
                     /locus_tag="ACHKYO_RS23145"
                     /old_locus_tag="pgaptmp_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HGU4431334.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
BASE COUNT         2054 a         2142 c         1861 g         1923 t
ORIGIN      
        1 cgaatagaaa acaaccattt cgacatcaac aatcgcttac attcgtttat atattgacca
       61 caactgatac atcagattat gtgatgactc gtgcttagat caatttttgc aatcattagc
      121 aaaaagatta ataaaccatc tatatcaatt tatccaacct attctgccgt tcaagaaatc
      181 gccgaacagt tatttttaac aaatttttct cttcccattg actttcccgg acaccttgtc
      241 tgacctaagg tgcgcgaaag ccactttttc cttcctgagt tatccacaaa gttatgcact
      301 tgcaagaggg ccattttcac actatcttgc agtgaatccc aaacataccc cctatatata
      361 gtgttctaag cagcttccct tactacaggt agtctgcatg aaactattgc ggaaagaatt
      421 ccaaaaacag gtacgacata catgaatcag aatctgctgg tgacaaagcg cgacggtagc
      481 acagagcgca tcaatctcga caaaatccat cgcgttctgg attgggcggc agaaggactg
      541 cataacgttt cgatttccca ggtcgagctg cgctcccaca ttcagtttta tgacggtatc
      601 aagacctctg acatccacga aaccattatc aaggcggccg cagacctgat ctcccgtgat
      661 gcgccggatt atcagtatct cgccgcgcgc ctggcgatct tccacctgcg taaaaaagcc
      721 tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg tgaaaatggt cgagatgggc
      781 aaatacgata atcatctgct ggaagactac acggaagaag agttcaagca gatggacacg
      841 tttatcgatc acgaccgtga tatgaccttc tcttatgctg ccgttaagca gctggaaggc
      901 aaatatctgg tacagaaccg cgtgaccggc gaaatctacg aaagcgccca gttcctttat
      961 attctggttg ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct gcaatatgtg
     1021 aagcgttttt acgacgcggt ttccacattt aaaatttcgc tgccgacgcc aatcatgtcc
     1081 ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg cggtgacagt
     1141 ctggattcca tcaacgctac ctccagcgcg attgtgaaat acgtttccca gcgtgccggg
     1201 atcggcatca acgccgggcg tattcgtgcg ctgggtagcc cgattcgcgg tggtgaagcg
     1261 ttccataccg gctgtattcc gttctacaaa catttccaga cagcggtgaa atcctgctct
     1321 cagggcggcg tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca tctggaagtg
     1381 gaaagcctgc tggtgttgaa aaacaaccgt ggtgtggaag gcaaccgcgt gcgccatatg
     1441 gactacgggg tacagatcaa caaactgatg tatacccgcc tgctgaaagg tgaagatatc
     1501 accctgttca gcccgtccga cgtaccgggg ctgtacgacg cattcttcgc cgatcaggaa
     1561 gagtttgaac gcctgtatac caaatatgag aaagacgaca gcatccgcaa gcagcgtgtg
     1621 aaagccgttg agctgttctc gctgatgatg caggaacgtg cgtctaccgg tcgtatctat
     1681 attcagaacg ttgaccactg caatacccat agcccgtttg atccggccat cgcgccagtg
     1741 cgtcagtcta acctgtgcct ggagatagcc ctgccgacca aaccgctgaa cgacgtcaac
     1801 gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg cgcaattaat
     1861 agcctggatg aactggaaga gctggcaatt ctggcggttc gtgcacttga cgcgctgctg
     1921 gattatcagg attacccgat cccggccgcc aaacgtggag cgatgggtcg tcgtacgctg
     1981 ggtattggtg tgatcaactt cgcttactac ctggcgaagc acggtgtgcg ttactccgac
     2041 ggcagcgcca acaacctgac gcataaaacc ttcgaagcga ttcagtatta cctgctgaaa
     2101 gcgtctaacg agctggcaaa agagcaaggc gcgtgcccgt ggtttaacga aaccacttac
     2161 gcgaaaggga tcctgccgat cgatacctat aagaaagatc tggataccat cgctaatgag
     2221 ccgcttcatt acgactggga agcattgcgt gaatcaatca aaacgcacgg cctgcgtaac
     2281 tccacgcttt ctgccctgat gccgtcagag acttcttcac agatctctaa cgccactaac
     2341 ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt cgaaagacgg tattttgcgc
     2401 caggtggtgc cggactacga gcacctgcac gacgcctatg agctgctgtg ggaaatgccg
     2461 ggtaacgatg gctacctgca actggtaggt atcatgcaga aatttatcga tcagtcgatc
     2521 tctgctaaca ccaactacga tccgtcacgt ttcccgtcag gaaaagtgcc gatgcagcag
     2581 ttgctgaaag acctgctcac cgcctacaaa ttcggggtca aaacgctgta ttatcagaac
     2641 acccgtgacg gcgctgaaga cgcgcaggac gatctggtac cgtcaatcca ggacgatggc
     2701 tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg gatgcagcgt gaacgcctta
     2761 tccggcctac ggctcggatt tgtaggcctg ataagacgcg tcagcgtcgc atcaggcaca
     2821 ggatgcggcg taaaatgcct tatccggcat taaactccca acaggacaca ctcatggcat
     2881 ataccacctt ttcacagacg aaaaatgatc agctcaaaga accgatgttc tttggtcagc
     2941 cggtcaacgt ggctcgctac gatcagcaaa aatatgacat cttcgaaaag ctgatcgaaa
     3001 agcagctctc tttcttctgg cgtccggaag aagttgacgt ttctcgcgac cgtatcgatt
     3061 accaggcact gcctgaacac gaaaaacaca tctttatcag caacctgaaa tatcagacgc
     3121 tgctggattc cattcagggc cgtagcccga acgtggcact gctgccgctg atctcgatcc
     3181 cggaactgga gacctgggtc gaaacctggg cgttctcaga aacgattcat tcccgttcct
     3241 atactcatat cattcgtaat atcgttaacg atccgtctgt tgtgtttgac gatatcgtca
     3301 ccaacgagca gatccagaaa cgtgcggaag ggatctccag ctattacgat gagctgatcg
     3361 aaatgaccag ctactggcat ctgctgggcg aaggtaccca caccgttaac ggtaaaactg
     3421 tgaccgttag cctgcgcgag ctgaagaaaa aactgtatct ctgcctgatg agcgttaacg
     3481 cgctggaagc gattcgtttc tacgtcagct ttgcttgttc cttcgcattt gcagaacgcg
     3541 aattgatgga aggcaacgcc aaaattattc gcctgattgc ccgcgacgaa gccctgcacc
     3601 tgaccggcac ccagcatatg ctgaatctgc tgcgcagcgg cgcggacgat cctgagatgg
     3661 cggaaattgc cgaagagtgt aagcaggagt gctatgacct gtttgttcag gcagctcaac
     3721 aggagaaaga ctgggcggat tatctgttcc gtgacggttc gatgattggt ctgaataaag
     3781 atattctctg ccagtacgtt gaatacatca ccaatatccg tatgcaggca gtcggtttgg
     3841 atctgccgtt ccagacgcgc tccaacccga tcccatggat caacacctgg ctggtgtctg
     3901 ataacgtgca ggttgctccg caggaagtgg aagtcagttc ttatctggtc gggcagattg
     3961 acgcggaagt ggacaccgac gatttgagta acttccaact ctgatggccc gcgttaccct
     4021 gcgcatcact ggcacacaac tgctgtgcca gaatgaacac ccttcccttc tggcggcgct
     4081 ggaatcccac aatgtggcgg ttgagtatca gtgtcgcgaa ggttactgcg gctcctgtcg
     4141 cacccgtctg gttgcaggtc aggttgactg gattgccgaa ccgttagcct ttattcagcc
     4201 gggggaaatt ttgccctgtt gttgccgggc aaaaggcgat attgaaatcg agatgtgaat
     4261 tggtcgtagt gccagataca acgcttatgc gtcttatctg gcctacaacg attacatggc
     4321 gtagtaatac gctttcacct gctcccagtc cgctttgggg attggctcca gatatttttc
     4381 cagctggcgg aagtcatgat taatcgcttt atcgcgacgc aagcggcgac gacttttctc
     4441 caggtcaaga aaaccagctt ctgcattacc ttctgttttc acatagatat ggcgaacata
     4501 acaacagcca tgctgacgat taatgctatg cattttctta aacgccagcg ccactgcttt
     4561 caacatggct tgccgtactt cgtcagaata aggcgatact gcatgctggg cataccagtc
     4621 agcaatgctg atgaaccccg ccatatcttc agtcaccagc aacgctcgcc attcaccctc
     4681 aattttcacc gcttcaccaa aaacgatctt cggcacgatg acaccggccc gttccagttc
     4741 tttaattacc gcaacctcac ggacaatcgt tggtcggccg aacggataac gtacggaatg
     4801 aaacagatga tgcgtcatgc gctttacata cagctttttg ccgttgcgct cgacacattg
     4861 caccccgctc ataccattac ggcgatagtt aggctcttca acccagtcgc cctctgttgc
     4921 ccaccagtga ttaaattcgt cgtactttgc tgaaactgcc ataccctatc gcctgtcatt
     4981 tttattaacg acaatgacta taggtggtta cctgaggaaa atcttaatga aacgtgtcgt
     5041 attaatgaat ttcttttgcc actactagcg tgacaccgct tttacccttc atttgcagcg
     5101 catagattcc accagccact accactaata gtgcgcccag tagataaaat gtgaatttaa
     5161 tcagtcggat acagcgatga agtttattcg acagggatta ggcatcgcct tacaaccaga
     5221 gttaacgctg aaaagcattg caggtgaatt gtgttccgtt cctcacgaac caaccttcta
     5281 tcgacagatt tcgttgttgg ctaaagaaaa gccggtagaa ggcagtccac tgtttttact
     5341 acaaacgtgt acggaacaac tcgtggtgaa tggaaaaatt tgatgtgaga caggatggcg
     5401 catgaacgcc atcctgcagt agcattactc tttattaagg aattttactg ccttatcagg
     5461 gaaatcagta aacagcccat ttacacccgc tttgttatac agagcatcat ataactgatt
     5521 cacatcagta gtgtattcag gcagtttatc tgaccgcacg gtataaggat gcactaccag
     5581 tttattctgc tgagcatctt gcaccatgcc agtgagtttg atattacccg gctgcgatgt
     5641 ttcctcaatc aacatatggt aatccggccc gataccgtcg gcatattccg ccacctgttt
     5701 catagcaccc ggcttaaaca tccagtcgta gttgtaatta acccagcttc catccggctg
     5761 tttctgctgc gtttcattcc agtcggtata ggcaatcagc tgtaccagat tgagatccat
     5821 gcccattttg ggttccagct cattcttaat acgcttcagc tcatcagcat caaaacattg
     5881 caaataaact ttgtcgtcct taccggtgta accatatttc ttcagcactt ccagcgtttt
     5941 tgccgcaata tccttccctt cctgatgatg gaaccacggc gctttaattt ctggatagat
     6001 accgatattt ttcccggtag agtgatttaa cccctgaaca aattcaatct cttcttcaaa
     6061 ggtgtgcacc cggaagtcgg acttacccat tgggaaacgc cccggataag tctgtacttt
     6121 tttaccgttt tcaatatcga aaccttcggt aaatttcagc gacttaattt catccagcgt
     6181 gaaatctatc gcgtagtaac gaccgtcttt gcgcgcccga tccgggaaac gatcggcaac
     6241 atcagtaaca cgatcgagat aatggtcatg cagaacaacc agatggtcgt ctttggtcat
     6301 caccaaatcc tgttccagat aatccgctcc ctgcgcatac gccatcgctt ttgctggcag
     6361 cgtatgctcc ggcaaatatc cactggcacc gcgatgggcg atgactattt tttcgttgct
     6421 gtccgccgcc attgcactgc ttcccatgac tatagtgctc atcatgatcg ccatgctaag
     6481 gtttttcagc ttcaatttca tgccattagc ctccgttgcg tttttgcagt aattgttcat
     6541 ggcgacgttt ttcgccaatc atcacaacaa tcaacaagat aaccgccaga atgctgccgc
     6601 caatcattac cataaagccg ccatcccagc cgaagaagtc cacggtgtag ccaacaatcg
     6661 cgctcgccgc caccgaaccg cccaggtaac caaacagccc ggtaaagccc gctgccgtac
     6721 ctgccgcttt tttcggtgcc agttccagcg catgcagacc gatcagcatc acaggaccgt
     6781 agatcaggaa gccgataaca atcatacaaa tcatatcgac ggttgggtta cctgccgggt
     6841 tcatccagta aacgatagtc gcgatggtca ccagtgtcat aaagaaaacg ccggttgccc
     6901 cacggttgcc acggaagact ttatccgaca tccagccgca cagcagggtg cccggaatac
     6961 ccgcatactc atagaagaag taggcccagg aagatttatc cagcgcgaaa tgtttaacct
     7021 ctttcaggta agtcggtgac cagtcgagga tgccgtaacg cagcagataa acgaacacgt
     7081 tggcgatggc gatataccac agcagtttgt tcggcagtac gtactgcatg aagatttgct
     7141 tcgccgtcag ctcctgttcc gctttttcgt tatagtcgtc cggataatca tttttgtact
     7201 cttcgatcgg cggcaagcca caggattgcg gggtatcgcg catcatcgca aaggcgaata
     7261 atgccaccag aatggcgcag aaagcaggca tatagagcgc cgcatgccag tcattgaacc
     7321 aggccatccc cagcaggaac agcagcggcg gaataccacc accgacgttg tgcgcacagt
     7381 tccacactga cacaatgccg ccacgttctt tctgcgacca ccagtgcacc atagtacgac
     7441 cacacggcgg ccaccccatc ccctggaacc aaccgcagag gaacaacagt acaaacatca
     7501 ccgcaatgct cgacgtcgcc catggcacaa agcccataaa caacatcact gccgccgcca
     7561 gaatcaaacc tgcgggcagg aaaacgcgcg gattcgagcg atccgatacc gaacccatta
     7621 tgaatttcga aaatccataa gcgatcgaga tccccgaaag cgcgaaacct aaatcaccgc
     7681 gagagaatcc ctgctcgacc agataaggca tagcaagcgc aaagttctta cgaaccaaat
     7741 agtaagccgc atagccaaag aatatcccca ggaaaatttg ccagcgcaat cgacgataag
     7801 tcggatcgat ctccgcagca ggtaagcgcg ctttgtgtgg cgctggttta aaaatactca
     7861 acattgaaag cctccgtggc ccgtggtctt atttatgatt aacagactga ttcagtggga
     7921 gaacctgccg tttctttagt tgccgcgatg ttaagaaaac attcagaaat taaatgtgaa
//
LOCUS       DAZGAN020000171         7865 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGAN020000171 DAZGAN020000000
VERSION     DAZGAN020000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.171"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            148..798
                     /gene="csgD"
                     /locus_tag="ACHKYO_RS23150"
                     /old_locus_tag="pgaptmp_004630"
     CDS             148..798
                     /gene="csgD"
                     /locus_tag="ACHKYO_RS23150"
                     /old_locus_tag="pgaptmp_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HGU4431335.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            803..1192
                     /gene="csgE"
                     /locus_tag="ACHKYO_RS23155"
                     /old_locus_tag="pgaptmp_004631"
     CDS             803..1192
                     /gene="csgE"
                     /locus_tag="ACHKYO_RS23155"
                     /old_locus_tag="pgaptmp_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HGU4431336.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1217..1633
                     /gene="csgF"
                     /locus_tag="ACHKYO_RS23160"
                     /old_locus_tag="pgaptmp_004632"
     CDS             1217..1633
                     /gene="csgF"
                     /locus_tag="ACHKYO_RS23160"
                     /old_locus_tag="pgaptmp_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HGU4431337.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1660..2493
                     /gene="csgG"
                     /locus_tag="ACHKYO_RS23165"
                     /old_locus_tag="pgaptmp_004633"
     CDS             1660..2493
                     /gene="csgG"
                     /locus_tag="ACHKYO_RS23165"
                     /old_locus_tag="pgaptmp_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HGU4431338.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2557..3048)
                     /gene="ycdZ"
                     /locus_tag="ACHKYO_RS23170"
                     /old_locus_tag="pgaptmp_004634"
     CDS             complement(2557..3048)
                     /gene="ycdZ"
                     /locus_tag="ACHKYO_RS23170"
                     /old_locus_tag="pgaptmp_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGU4431339.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3150..3704)
                     /gene="ycdY"
                     /locus_tag="ACHKYO_RS23175"
                     /old_locus_tag="pgaptmp_004635"
     CDS             complement(3150..3704)
                     /gene="ycdY"
                     /locus_tag="ACHKYO_RS23175"
                     /old_locus_tag="pgaptmp_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HGU4431340.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3728..4465)
                     /gene="ycdX"
                     /locus_tag="ACHKYO_RS23180"
                     /old_locus_tag="pgaptmp_004636"
     CDS             complement(3728..4465)
                     /gene="ycdX"
                     /locus_tag="ACHKYO_RS23180"
                     /old_locus_tag="pgaptmp_004636"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HGU4431341.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4520..5458)
                     /gene="ghrA"
                     /locus_tag="ACHKYO_RS23185"
                     /old_locus_tag="pgaptmp_004637"
     CDS             complement(4520..5458)
                     /gene="ghrA"
                     /locus_tag="ACHKYO_RS23185"
                     /old_locus_tag="pgaptmp_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HGU4431342.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFSGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5692..5779
                     /locus_tag="ACHKYO_RS23190"
                     /old_locus_tag="pgaptmp_004638"
     tRNA            5692..5779
                     /locus_tag="ACHKYO_RS23190"
                     /old_locus_tag="pgaptmp_004638"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5726..5728,aa:Ser,seq:gga)
     gene            5824..5960
                     /locus_tag="ACHKYO_RS23195"
                     /old_locus_tag="pgaptmp_004639"
     ncRNA           5824..5960
                     /ncRNA_class="other"
                     /locus_tag="ACHKYO_RS23195"
                     /old_locus_tag="pgaptmp_004639"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(6153..7139)
                     /gene="ycdU"
                     /locus_tag="ACHKYO_RS23200"
                     /old_locus_tag="pgaptmp_004640"
     CDS             complement(6153..7139)
                     /gene="ycdU"
                     /locus_tag="ACHKYO_RS23200"
                     /old_locus_tag="pgaptmp_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HGU4431343.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(7136..7663)
                     /locus_tag="ACHKYO_RS23205"
                     /old_locus_tag="pgaptmp_004641"
                     /pseudo
     CDS             complement(7136..7663)
                     /locus_tag="ACHKYO_RS23205"
                     /old_locus_tag="pgaptmp_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            7730..>7865
                     /locus_tag="ACHKYO_RS23210"
                     /old_locus_tag="pgaptmp_004642"
     CDS             7730..>7865
                     /locus_tag="ACHKYO_RS23210"
                     /old_locus_tag="pgaptmp_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4431344.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     Q"
BASE COUNT         2223 a         1941 c         1761 g         1940 t
ORIGIN      
        1 agatgtaatc cattagtttt atattttacc catttagggc tgatttatta ctacacacag
       61 cagtgcaaca tctgtcagta cttctggtgc ttctatttta gaggcagctg tcaggtgtgc
      121 gatcaataaa aaaagcgggg tttcatcatg tttaatgaag tccatagtat tcatggtcat
      181 acattattgt tgatcactaa accgtctttg caagcgacag ctctcttgca gcaccttaaa
      241 caatcgctgg caattacagg aaaattacat aatattcaac gttctctgga cgatatatct
      301 tcaggctcta ttattcttct ggatatgatg gaagcagata aaaagcttat ccattattgg
      361 caggatactt tgagcaggaa aaacaacaat atcaaaatat tgttgctaaa tacgcctgaa
      421 gattacccgt accgcgacat tgaaaactgg cctcatatca acggcgtttt ttatgccatg
      481 gaggatcaag aacgtgttgt caatgggttg caaggcgtcc tgcgcggcga atgctacttt
      541 acgcaaaagc ttgccagtta cctgattaca cattctggta actatcgtta taacagcacg
      601 gaatcagccc tccttactca tcgggaaaaa gagatcctga ataagctgcg tatcggcgcg
      661 tctaataacg agatcgctcg ttcgttgttc atcagcgaaa atacggttaa aacgcatctt
      721 tataatcttt tcaagaagat agccgtaaaa aaccggacac aagcggtttc ctgggcaaac
      781 gataacctca ggcgataaag ccatgaaacg ttatttacgc tggattgtgg cggcagaatt
      841 tctgttcgcc gcagggaatc ttcacgccgt tgaggtagaa gtcccgggat tgttaactga
      901 ccatactgtt tcatctattg gccatgattt ttaccgagcc tttagtgata aatgggaaag
      961 tgactatacg ggtaacttaa cgattaatga aaggcccagt gcacgatggg gaagctggat
     1021 cactataacg gtcaatcagg acgttatttt ccagactttt ttatttccgt tgaaaagaga
     1081 cttcgagaaa actgtcgtct ttgcactgat tcaaactgaa gaagcactaa atcgtcgcca
     1141 gataaatcag gcgttattaa gtacgggcga tttggcgcat gatgaattct aaataaaaaa
     1201 ttgttcggag gctgcaatgc gtgtcaaaca tgcagtagtt ctactcatgc ttatttcgcc
     1261 attaagttgg gctggaacca tgactttcca gttccgtaat ccaaactttg gtggtaaccc
     1321 aaataatggc gcttttttat taaatagcgc tcaggcccaa aactcttata aagatccgag
     1381 ctataacgat gactttggta ttgaaacacc ctcagcgtta gataacttta ctcaggccat
     1441 ccagtcacaa attttaggtg ggctactgtc gaatattaat accggtaaac cgggccgcat
     1501 ggtgaccaac gattatattg ttgatattgc taaccgcgat ggtcaattgc agttgaacgt
     1561 gacagatcgt aaaaccggac aaacctcgac catccaggtt tcgggtttac aaaataactc
     1621 aaccgatttt taagccccag cttcataagg aaaataatca tgcagcgctt atttcttttg
     1681 gttgccgtca tgttactgag cggatgctta accgccccgc ctaaagaagc cgccagaccg
     1741 acattaatgc ctcgagctca gagctacaaa gatttgaccc atctgccagc gccgacgggt
     1801 aagatctttg tttcggtata caacattcag gacgaaaccg ggcaatttaa accctacccg
     1861 gcaagtaact tctccactgc tgttccgcaa agcgccacgg caatgctggt cacggcattg
     1921 aaagattctc gctggtttat accgctggag cgccagggct tacaaaacct gcttaacgag
     1981 cgcaagatta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa ccgaatcccg
     2041 ctgcaatctt taacggcggc aaatattatg gttgaaggtt cgattatcgg ttatgaaagc
     2101 aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga cacgcaatac
     2161 cagctcgatc agattgccgt gaacctgcgt gtcgtcaatg tgagtaccgg tgagatcctc
     2221 tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg ggttttccgc
     2281 tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa cgaacccgtt
     2341 atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa tgatggtatc
     2401 gaccgtggtc tgtgggattt gcagaacaaa gcagaacggc agaacgacat tctggtgaaa
     2461 taccgccata tgtcggttcc accggaatcc tgacagataa aaaagccgga attatcatcc
     2521 ggcattttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt tgatttcctg
     2581 ctcacggtgc gccgtctttg cactacgcgc cgccagccac aggccgctgt ttttcattgc
     2641 gtaaccaaat accagcccaa gcgccagcga aggtaacacc agtttccagt caccctgccc
     2701 ggcaaacgtc gcgcatgcgc ctataaacgt tcccggaaca aatgaaagca gcagctgttt
     2761 ggcctgaata cacatcagaa acgcgacaat accggttatg acataaccga gaatttccag
     2821 atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc cactaagcag
     2881 ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaggcaa agtaggccgt
     2941 acagcccagg aagcccgccc agcttagtaa gcccagggaa acagccaccc atccccagat
     3001 accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc cgcgcatgat
     3061 agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa caaattatct
     3121 gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc cagctcgtcc
     3181 cacattgcac taatagcatc acgggttagc ggtgccatgg tgcgccagaa aggcgtggtt
     3241 gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata ctcactgaac
     3301 agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca ggaagccgca
     3361 agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtggcatccc tcgttcggaa
     3421 agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg atatggcggc
     3481 acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg cgccatatca
     3541 cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt cgcagccagt
     3601 ttcccctcac gaatcagggt aaacagcggc accagtaaag gatcttgcgg ttggcggtaa
     3661 tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat atttccatta
     3721 ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat tcaaggaagt
     3781 tcagtaagcg gcgcggagaa acattcaaaa tgcgctctgg cggaaaatct accgcgtcaa
     3841 ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa tccgagccta
     3901 atgccaccca acctccagca tcacgtaccg ctgcggctac cgcacgacag ttgtcttcac
     3961 tgcccttacg tgagtgtaaa aatgaagaat tattgatttc cagcgccacc tgatgtttcg
     4021 cggctgcttc agcaacggct ttcacatcta tttcatattt gggatttccg ggatggctta
     4081 ttatatgcac attgccgctg gcgatagtgg cgatcatcgc ttgtgtattg gtcgctttgt
     4141 catgtggtgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc gagtcaaaca
     4201 ttttaccgct gcaatcaatt tcaccatcaa catttttaat gttagcttcg atgccgcgca
     4261 ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag tgccagtgat
     4321 gcggagcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta atgccctttt
     4381 gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg gcaacggtat
     4441 gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg ccaaggatag
     4501 caggaatcct gatgctttat tagtagccgc gtgcgcggtc gacttgcccg cagaccctct
     4561 cccctttttc gagctgggca atggtgcgag aaatgtactc cacagcttca gcgggacggg
     4621 taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc ggactttcag
     4681 gcggtaaggg ttcacgatta aaaacatcca acattgcgcc tttaactttg ccgctatcca
     4741 gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg aggagatacg
     4801 cgccatccgg taatttttcg agtaattgtt gattaataat gccgacggtt tcaggggtat
     4861 tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt tcttcccgtc
     4921 cggaaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa cgcagcggaa
     4981 agcgccaggt ttgcagactc tgagcaactt tactgcccag tacgcctgcg cccaaaatgc
     5041 cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa ctattttgct
     5101 ggatgcgata atcgtcaaaa cgtcgaaacc aatgcagcac ctgactgaca gcatattcct
     5161 gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa gggttcagca
     5221 tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg agtgcgaaca
     5281 ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact aaagcataat
     5341 cagcagagtc attatctccg cttttccatg ctctgactct tgcctgagga atagctttgc
     5401 gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg atatccatac
     5461 tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat ctacaaaggc
     5521 caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt attaaaacca
     5581 cacattgatt gaagtttgaa taaacgcgcg attttttcaa aaagtttgtt gacctcaggt
     5641 cgtgatttcc ctaaattagc gcccgttcca gcaagacagg aacgacaatt tggtgaggtg
     5701 tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat cgggggttcg
     5761 aatccccccc tcaccgccat atttaaagaa gagctcgtac gaaagtacgg gctttttttt
     5821 cgtatattgc acacaccggg gggatgagaa gccccgaccg gggttcgaca actggcgaca
     5881 gccagttgga cagaccgtga acgcagtgag cgggctgccc gcagggcgag cgaagcgagt
     5941 caatcccccc ctcaccgcca tatttaaaga agagcccgta cgaaagtacg ggcttttttt
     6001 tcgtatattg cacacaccgg gtgcccccgc ccacgtctgt tagggcgagg ggaaattgtg
     6061 cacatcaact gtctggttac tcagtcaaca atcaaaatgc gcaagatagt agagaacata
     6121 tatgcgttat ctggtcactt cctgtaactt cctcacttat taaaatgccc ccaacggaaa
     6181 tacatgtcac atgccatcat aaaatcgtta tgccagagga atcgacaatg gaaaaaatag
     6241 taaattaagc acggtacata gcagagtaaa aaataggctt caaaccaaat ccgccattcc
     6301 acaagcgttg ccaataccga caccagcgta atccacatca tttcaataac cagcaatatg
     6361 aataaccgaa gccaaagcgg cttggcaaaa aaggtcgaca acgcattttc actggagaaa
     6421 aacagatcga tttcctgcaa aaaaagcctg cccagaaaag cgtaccgctt ggagaagcgc
     6481 agaaaaaaca gattaatcgc caacactacg ccacaaagaa tgagcaatgc aattatcgat
     6541 ttaaacgtta accactgccc ctctgtcaca aaagaatcac tgttacgcaa cgggctgata
     6601 gtaagcaggt taacaaccgt tgagatgcac accgaaagca gcacatagcg agtcagactt
     6661 ttatatttta cttttttcgc tactgaaata gcgacctgct tgacataact ggtgcggatc
     6721 atccgaaaca gcagaaagat gaccaacggc ccaagtaacg aaatcaggaa cagcgcatat
     6781 aacgagggta gaagctggta tagcccccaa ctcattgcac tcaacatgat taacgacaga
     6841 aatcctgcca ccagaggttg tgtaagaaat aattctattt tgtggttctg ctttttctta
     6901 aaccacgaaa aatcgatatt cttcgtgacg catttataag cccagtacgc cttgagatca
     6961 aaaataaaat tatacagcac tagggccaga atcccactaa ctacaccaat atcctctgat
     7021 ggaaaagtaa accctgcatt cttccaataa aagcctatca gaataagtcc aatagcataa
     7081 atgtatatca gatttcgttt atcctgaaag cgaataaaag ttaaataatc aggaatcatt
     7141 tgaatacctt tgcatcacta aatgctttac gacaatgcat aactcgttgc tgatccacgc
     7201 cagcaaaatc atttaacata ttccccaaat agggcttatt acttatttca aggaaagcta
     7261 ccggggttgg catattcaga aatgtatcga aagattcagt ccatttcacc gtatgagtta
     7321 agtgtaatga gagattttct ttgataattg acggagcgac ctcgcttttt gctgttacgt
     7381 tcatcagaac ctgatgctct ggcgaagcga tatccagtcc ggcaagataa tcgcgcattg
     7441 cctgaacgcc gtcttccatc aaacgcgtgt gccaggcacc gctcacaccg agtttaaccg
     7501 gttcgtatcc agcggccatc agcagcgtgg caaattcatt caacgcagcc tgcgttcccc
     7561 caataacctg ctggcgcggc gtgttatcac agctaatatc cagcgcaatg cctgattccg
     7621 taatcatcgt ctgcagttgt tcgcggttga tgcctttaac cgcctgatct tacccagcaa
     7681 tagtggacac gcggctaagt gagtaaactc tcagtcagag gtgactcaca tgacaaaaac
     7741 agtatcaacc agtaaaaaac cccgtaaaca gcattcgcct gaatttcgca gtgaagccct
     7801 gaagcttgct gaacgcatcg gtgttactgc cgcagcccgt gaactcagcc tgtatgaatc
     7861 acagc
//
LOCUS       DAZGAN020000172         7785 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGAN020000172 DAZGAN020000000
VERSION     DAZGAN020000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.172"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..260)
                     /locus_tag="ACHKYO_RS23215"
                     /old_locus_tag="pgaptmp_004643"
     CDS             complement(<1..260)
                     /locus_tag="ACHKYO_RS23215"
                     /old_locus_tag="pgaptmp_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU4431345.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKA"
     gene            473..1264
                     /locus_tag="ACHKYO_RS23220"
                     /old_locus_tag="pgaptmp_004644"
     CDS             473..1264
                     /locus_tag="ACHKYO_RS23220"
                     /old_locus_tag="pgaptmp_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HGU4431346.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            1274..2116
                     /locus_tag="ACHKYO_RS23225"
                     /old_locus_tag="pgaptmp_004645"
     CDS             1274..2116
                     /locus_tag="ACHKYO_RS23225"
                     /old_locus_tag="pgaptmp_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGU4431347.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            2126..2902
                     /locus_tag="ACHKYO_RS23230"
                     /old_locus_tag="pgaptmp_004646"
     CDS             2126..2902
                     /locus_tag="ACHKYO_RS23230"
                     /old_locus_tag="pgaptmp_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HGU4431348.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNIPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            2912..4453
                     /locus_tag="ACHKYO_RS23235"
                     /old_locus_tag="pgaptmp_004647"
     CDS             2912..4453
                     /locus_tag="ACHKYO_RS23235"
                     /old_locus_tag="pgaptmp_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HGU4431349.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHIMAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLSMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            4450..6531
                     /locus_tag="ACHKYO_RS23240"
                     /old_locus_tag="pgaptmp_004648"
     CDS             4450..6531
                     /locus_tag="ACHKYO_RS23240"
                     /old_locus_tag="pgaptmp_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HGU4431350.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            6552..7028
                     /locus_tag="ACHKYO_RS23245"
                     /old_locus_tag="pgaptmp_004649"
     CDS             6552..7028
                     /locus_tag="ACHKYO_RS23245"
                     /old_locus_tag="pgaptmp_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HGU4431351.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPR"
     gene            7071..>7785
                     /locus_tag="ACHKYO_RS23250"
                     /old_locus_tag="pgaptmp_004650"
     CDS             7071..>7785
                     /locus_tag="ACHKYO_RS23250"
                     /old_locus_tag="pgaptmp_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HGU4431352.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIVLIMGGVL"
BASE COUNT         1867 a         1893 c         2048 g         1977 t
ORIGIN      
        1 atagcttttt gtggcggaag atctgagaaa tcaagggcct tttctttttg tcgaatatcg
       61 ttccaggtat attccagcac ttcgataaat aaattttctt tattaccaaa gtggtgataa
      121 accgtctgtt tactacaccc tgctgccttt acaatattat ctactctggc cccttcataa
      181 ccatattcgg caaattcatt aaccgcacat agcagcaatt tttccttcag gctctgtacg
      241 ttgctggtat caatatacat aacgcttccc cacgacctct tcaagataac tatcttaacc
      301 cgattaatta gaagctccag taaaaaggat aaaaacttgt actttcatca agttatcaaa
      361 tgtactgtga tcactatcgc atatcaacca accagatggt tgacctttct tgcggcacgt
      421 cgcaagctaa ttgtcactta gaaaacaata ttcaaaaaaa gagagctatt gtatgaaatc
      481 cactcgtcct gttgccgtga ttaccggagc cgctcgtggt attggaaagg gctgtgcgct
      541 ggagcttgct cgtggcggat ttaacctgct gattaacgat cgtcccgatg ccgacagcgt
      601 agaaaaactg catatcacac aacaagaatg tttggcagaa ggcgtggaag tcatttgctt
      661 ccctgccgat gtcggcgatc tctccttgca tgaagagatg ctcgacgcgg cgcaaaacca
      721 gtgggggcgg ctggactgtc tgctcaataa cgcggggatt tcagtgaaaa aacgcggtga
      781 cctcctcgac ctcgaaccag acagcttcga tcaaaacatt gccattaaca cccgcgcgcc
      841 gttcttcctt gctcaggcat tcagtaagcg cttactggca cagccgaaac cggaagcgga
      901 gctgccgcac cgctcgatta ttttcgtcag ttccatcaac gccatcatgc tggcgatgaa
      961 tcgcggcgaa tacaccattg ccaaaaccgc cgtttctgcc gctgctcgtc tgtttgctgc
     1021 tcgcctttgt aatgaacaaa ttggcgttta tgaagtgcgc cctggtctca tcaaaaccga
     1081 tatgactatt ccggcaacgg cttattacga cgaactgatt gccaaaggtc tggtgccgtg
     1141 gggacgttgg ggttatccgg ctgatattgc ttccactgtc cgcgcgatgg cggaaggtaa
     1201 actgatttac acctgtggtc aggcggtcgc catcgacggc ggcttaagta tgccgcgctt
     1261 ctgaggctga atcatgagag atctgttaca acgcccggat ttattcagta tcaataccgc
     1321 aacactgggc tataaaacgc cgctacccgc cattattgat gcctgcgccg cgcgtggtat
     1381 tggcgcgatt gctccctggc gcagggagtt gcaaggtgaa gatctccagc aaatcgccca
     1441 ccagcttgcc gccagtaaca tgagcgtttc cggtttgtgc cgtagcactt actacaccgc
     1501 gccgacactg gcggaacgca aactggcgat tgatgataac cgccgggcgc tggacgacgc
     1561 agcggtgctt aacgccgcct gttatatgca ggtggttggc ggtctgccaa cgggcacaaa
     1621 agatctgtac gaagcgcggg aacaagtaaa acaagggatt cgccagttgc tgccacattc
     1681 gaaagatgtc ggcgtgccaa ttgcgctgga gccactgcac ccgatgacag ctgccgatcg
     1741 ctcttgcctg tgcacgttac gccaggcgct ggactggtgt gatgaactcg atcccgatgg
     1801 tgagtttggc ctcggcgtgg cggtggatgt ttatcacgtc tggtgggatc cggatctcgc
     1861 cagtcagatc ctgcgtgccg gaaaacgcat cctcgcgttt catgtttccg actggttagt
     1921 gccgaccact gatctggtca atgaccgtgg tatgccggga gatggcgtca tcaatattcc
     1981 gtcaattcgt cggctggttg aaaacgccgg atttaatggt gctattgaac tggaaatatt
     2041 ctcgccttac tggtggcaga aagatattaa cagcacgctg gatattagcg tcgatcgcat
     2101 cgcgcattat tgttaaggaa tgatcatgag tgaccaaccc gttctgttca gcagagtggc
     2161 ggcttcctgc cgcctgacat tgaaccgtga agataaatgt catgctatta acgaagagat
     2221 gatcgaatct ctcgaccatt atttaaatga aattgaaaac gacaccacat tacgattagt
     2281 cgaattaacg gcaaccggcg ataaattctt ttgtgcgggc ggcgatatta aatcgtggtc
     2341 ggcctattcg ccattagata tgggcagaaa atggattaaa cgcggtaatg acgtttttaa
     2401 tcgcctgcga aacatacccc aattaaccgt tgcgaatctc aacggtcata ccatcggcgg
     2461 tggtattgaa ctggccttgt gctgcgatat tcgtattgcc cgccccggcg cgaaattctc
     2521 taacccggaa gtgatgctcg gcatggtgcc tggctggatg ggcattgaac gtgtgcttaa
     2581 tcaggttggt ccggtggtcg gtcgccagat gctgatgctg ggtaaacgcc tgacggcgca
     2641 ggaagcccag gcggcgaatt taattgatga agtggtagaa aaagaacagg tggaaagctg
     2701 gatggcgaac cagttagcgc agctggaaaa atgcggtccg gtggcactgg cgcatattaa
     2761 gcaactgatt ctggcactgg aaaataaaca tgccgattac ccgcaccagc tactggccgg
     2821 attaatgtct gccactcagg attgccagca ggcgacgcgc gcctttgccg aaaaaagtag
     2881 cgtgagcttc cataatcaat aaggaaattg tatgcgtaag ataacaaccg ccgaggcact
     2941 ggcggcgcaa attcaggacg gcgcaaccat tgccattagc ggtaacggcg gcggcatggt
     3001 ggaagctgac catattatgg ccgccattga agcgcgtttc ctgcaaaccg gacacccgcg
     3061 cgacctgacg ctaatccact cgctgggtat tggcgatcgc gactgcaaag gcactaaccg
     3121 ttttgcgcac gcagagatgc tcaaacgcat tattgccggg cactttacct ggtcgcccaa
     3181 aatgcaggcg ctggtaaaaa ataacacgat tgaagcctac tgctttcctg gtggcgtgat
     3241 tcaggcactg ctacgggaga tcggcgccgg acgtccgggg ctttttaccc acgttgggct
     3301 gggatcgttt gttgatccac gcaatggcgg cggtaagtcg aatgaatgca ctaccgacga
     3361 cctggtagaa ctgattgaaa tcgacggtga aaccaaactt cgttatcgcc ctttcaaagt
     3421 ggattacgcg attttgcgtg gcacttatgc cgatcctcga ggcaacgtca gccttgaaga
     3481 agaagcgatt gatatggata gctattccat ggcgctggca gcacacaaca gcggcggcaa
     3541 agtgttcgta caggtacgcg atgtgctgga agctggcgcg attgaaccac gccgggtcaa
     3601 attaccgggc attctggttg atggcatcgt tgaacaccgc gaacaaccac aaacctatct
     3661 tggcggttac gacctgacca tcagcggcca acatcgccgt ctgagttcta acgacgccat
     3721 tgaactggtt agtcatccgg tacgtcgcct gattgcccgt cgggcggcac gcgaactggt
     3781 agctggcgct tcaaccaact ttggctttgg tattcctggg ggtattccag gcgtagcgct
     3841 gcgcgaaggc gtgccttacc aaagtttatg gctgagcgtc gagcaaggcg tgcataacgg
     3901 tatgatgctg gatgatgcgt ttttcggctg cgcccgcaac gccgatgcca ttattccatc
     3961 actcgatcaa tttgagttct acagtggcgg agggatcgat atcaccttcc tcagcatggg
     4021 agagatggat cagtacggta acgtcaacgt ctcccatctc aatggcaatc tgattggccc
     4081 cggcggattt ctcgaaattg cgcaaaacgc ccgtaaagtg gtgttctgcg gcacgttcga
     4141 cgccaaaggt agcaagattg atgtaacgcc agatggcttg catatcaccc agtcaggtca
     4201 aatccctaaa ctggttaccc aggtggaaaa aatcactttt agcgccgcct acgcacagca
     4261 aagtggtcag gaagtgttgt atatcactga acgcgcagta ttccagttaa cggcagaagg
     4321 cgttgaatta attgaaatcg cgccgggtgt ggagattgag cgcgacattc tgccgtatat
     4381 ggctttccgt ccaattatca agcacccacg cctgatggaa agtagcctgt ttacgccgat
     4441 ggaggatgca tgagtcagct tgatgacacc atccttgatg cgctgacgca cgttactttc
     4501 ccaaagggtt ttgcacaggc agagcccgca tgggttgtca cggtagacgg tgttgattac
     4561 ccactctggc aaacagatgc cctggtagtc ggcagtggtg cagcaggatt acgtgcggca
     4621 gttgaactta aacgccgcca gcaaaatgtg ctgatcgcta ccgccgggtt atatatgggg
     4681 acgtcggcat gttctggttc cgataaacag acactattta ccgccgctac cgcgggcaac
     4741 ggcgacaact tcaccaaact ggcagaggca ctggcgagcg gtggggcgat ggatcacgac
     4801 accgcttatg tcgaagcggt aggttctctg cacactctag gcgggttgca atatcttggt
     4861 ctggaattac cggaagatcg ctatggcgcg attcttcgtt atcaaaccga ccatgacgaa
     4921 gccgggcgtg caacctcgtg tgggccgcgt acctcaaggt taatggtgaa agtgctgttg
     4981 gaagaagtac agcgcctcgc cattccagtg ttgaccagtg caacagtgat taaactgctg
     5041 catcagcgtg acgaaaacgg cgaagaccgc gtggcagggg caatcctcgc gaccggtcat
     5101 cgcgcccata acccatgggg gctggcaatt gtgactgcgc ccaatgtggt actggcaaca
     5161 ggagggcctg gcgagcttta tcgcgacagt gtttatccac ataaatgctt cggctcgctg
     5221 gggctggcgc tggaggaagg tctgacgcta accaatctga ccgaaagcca gtttggtata
     5281 ggcacgccgc gtagcacgtt tccgtggaat ttatccggca cctatgtaca ggtgatcccg
     5341 tatatctatt ccgtggatgc tgagggtaac gagtataact tcctcgcgga ttactatcgc
     5401 accacccagg agctggcttc aaacattttc cgtaaaggct atcagtggcc gttccacgcc
     5461 acgcgggtga tggattttgg ctccagcttg ttagatatgg cagtagcgca agagcagcaa
     5521 tcagggcgtc aggtatttat ggatttcaat cgcaatcctg aagcggtgcc gggtgacctg
     5581 ccgttctcat tagatcgact ggacgacgac gttcgcgcgt atctggaaaa taacgacgct
     5641 ctggcaccat cgcccatcga acggctgcaa cgaatgaatc cgctgtctat ctcgctgtat
     5701 aagatgcacg gttacgatct caccacacag cctttgcagt ttgccatgaa taatcagcat
     5761 atgaatggcg gcattgaagt ggatatctgg ggacaaacct ccctgcccgg ctgtttcgcc
     5821 gtgggggaag ttgctggcac acacggcgtc actcgccctg gtggtgcagc attgaatgcc
     5881 gggcaggttt ttgctgttcg tctggcacgt tttattggtt gcacgcaaaa gcggaatatt
     5941 gatggagata tagcacagct ggtagctcct acactggctt ctataagaga gatcattact
     6001 caggcgcacg ataacgggac cgggatgccg ttgtcggttg tgagagaaaa aattcaggca
     6061 cgaatgtctg accatgcggg atttatttgc catgccgata aagtccgacg cgccactcgc
     6121 gatgccctgc tattgagcga atttgtccaa cggcatggat tggctatcaa acatgtgggt
     6181 gaagttgccg agctgtttat gtggcggcat atggcactga cttctgccgc cgtattaacg
     6241 caattaacgc attatattga cgcaggtggt ggtagtcggg gagcacgtat tattctcgac
     6301 agggatggaa attctattcc gcaaacacga aatggttttt gtgatgcctg gcgctttcgt
     6361 tcagagcgta ctgaggataa aaaagataaa ctacttattc attattgcaa cggtatcttt
     6421 cacgtcagag aaacacctgt tcgtgagttt cctattatcc gaggtatttg gtttgaaaaa
     6481 aactggcctg gttttcttaa tgggacaatt taccagccac aggatgaatg atattgcaaa
     6541 ggttaatcac tatgcgaaat ataattagat ctattaatgc tctgcttgca gcattgaaca
     6601 ttaccatttt ggcgatcatc gtggcatgtg tgacgtggca ggtggctgca cgatttatat
     6661 ttactagccc cagtattttt acagatgaac tcagccgttt gctctttatt tgtcttggac
     6721 tttttggtgg ggcatacacc gcagggcaaa accggcatct ggcgattgac ctgctaccta
     6781 tgatgctgaa aggaaaagcc cgacgtcatt tatttttgtg tattcagatt atcgttatta
     6841 tcttcgcaac catcattatg gtatatggtg gtgggctatt aacgatggat acattcgatt
     6901 caggtcagac ctcgcccgct ttaggctggc aaatgggata tatctatatg agcatcccga
     6961 ttagtggtgt acttattatt atatacacca tagatatggt tttaaccgaa cttaaacaac
     7021 cacgctaaag ataacaaaca acaaccaacc atagtaggaa tggagaaatt atggattact
     7081 ggttaccgat tattgtgtta tttggtgcct tcttttttat gttggcatta ggtgtaccta
     7141 ttgtttatgc cattggttta tcgacactgg catcaatttc cacacaactc gatttcaact
     7201 cagcattgag tgtggtttcc caaaagttag cctccgggtt agatagtttt acgttgttgg
     7261 caatcccatt ttttatcttg tctggcaaca ttatgaatca tggaggaata gcgcgacgat
     7321 taatcaactt tgcccgtatt ctcggcggtc gtctgccagg ctcacttgcc cactgtaata
     7381 ttctcgccaa tatgctgttc ggggcaattt ctggttcagc cgtggcttcg gcggctgcga
     7441 tgggcggcgt catgcatccg caacaggtta aagagggcta cgacccggca ttcagtactg
     7501 cggttaacgt tgcctctgcc ccgacgggtt tgcttattcc accaagtaat acgttaatcg
     7561 tctattcact ggtcagtggt ggtacttcta ttgccgcact gttcctggcg ggttatgttc
     7621 cggggatttt gctgggtctg gcactgatgg taatagccgg tattatcgct gtacgtcgcg
     7681 gttatccaaa accagaacgt ccgactttgc gccaggctgg cgttgcgata tggatggcta
     7741 tccccagtat tttcctgatt gtgttaatta tgggtggcgt actaa
//
LOCUS       DAZGAN020000173         7696 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGAN020000173 DAZGAN020000000
VERSION     DAZGAN020000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.173"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..264
                     /locus_tag="ACHKYO_RS23255"
                     /old_locus_tag="pgaptmp_004651"
     CDS             <1..264
                     /locus_tag="ACHKYO_RS23255"
                     /old_locus_tag="pgaptmp_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein"
                     /protein_id="HGU4431353.1"
                     /translation="SQQLTSMLSVQQQRDRYMARQLLSAPVPSLLIAGGYHASKSMGV
                     PLHMEDLAAGTHPVVLMLAEKGMNITVDHADYVWFVAPDTTKR"
     gene            272..1048
                     /locus_tag="ACHKYO_RS23260"
                     /old_locus_tag="pgaptmp_004652"
     CDS             272..1048
                     /locus_tag="ACHKYO_RS23260"
                     /old_locus_tag="pgaptmp_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HGU4431354.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKRKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVSFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            1217..3478
                     /locus_tag="ACHKYO_RS23265"
                     /old_locus_tag="pgaptmp_004653"
     CDS             1217..3478
                     /locus_tag="ACHKYO_RS23265"
                     /old_locus_tag="pgaptmp_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU4431355.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSVNMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGHKPACDSRVICVPQGSYDIDD
                     KVGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLVVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            3547..4722
                     /gene="senB"
                     /locus_tag="ACHKYO_RS23270"
                     /old_locus_tag="pgaptmp_004654"
     CDS             3547..4722
                     /gene="senB"
                     /locus_tag="ACHKYO_RS23270"
                     /old_locus_tag="pgaptmp_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HGU4431356.1"
                     /translation="MNIFTLSKTPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSGDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(5352..5810)
                     /locus_tag="ACHKYO_RS23275"
                     /old_locus_tag="pgaptmp_004655"
                     /pseudo
     CDS             complement(5352..5810)
                     /locus_tag="ACHKYO_RS23275"
                     /old_locus_tag="pgaptmp_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612075.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(6250..6636)
                     /locus_tag="ACHKYO_RS23280"
                     /old_locus_tag="pgaptmp_004656"
     CDS             complement(6250..6636)
                     /locus_tag="ACHKYO_RS23280"
                     /old_locus_tag="pgaptmp_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HGU4431357.1"
                     /translation="MKFVSSQEMIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYH
                     YEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVAQVFLKRNGIHIISSRISFDEMQ
                     IIALNAATGEYNWKMVSDHLKAIILN"
     gene            complement(6633..6857)
                     /locus_tag="ACHKYO_RS23285"
                     /old_locus_tag="pgaptmp_004657"
     CDS             complement(6633..6857)
                     /locus_tag="ACHKYO_RS23285"
                     /old_locus_tag="pgaptmp_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGU4431358.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
     gene            6923..7278
                     /locus_tag="ACHKYO_RS23290"
                     /old_locus_tag="pgaptmp_004658"
                     /pseudo
     CDS             6923..7278
                     /locus_tag="ACHKYO_RS23290"
                     /old_locus_tag="pgaptmp_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(7511..7642)
                     /locus_tag="ACHKYO_RS23295"
                     /old_locus_tag="pgaptmp_004659"
                     /pseudo
     CDS             complement(7511..7642)
                     /locus_tag="ACHKYO_RS23295"
                     /old_locus_tag="pgaptmp_004659"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
BASE COUNT         2201 a         1800 c         1748 g         1947 t
ORIGIN      
        1 agccagcaac tgacgtcaat gctctctgtt cagcagcagc gggatcgcta catggcccgc
       61 cagcttctga gtgccccggt accgtcgttg ttgattgcag gtggttatca cgcgtcaaaa
      121 agcatgggcg taccgttaca catggaagat cttgccgccg gcacccatcc agtggtgttg
      181 atgcttgcag aaaaaggaat gaacatcaca gtagatcatg ctgactacgt gtggtttgta
      241 gcgccagata caacaaaaag gtaaaccatg gatgatgaat attctccact tcccacagtc
      301 ggtaaaatgg agcagctggt ttatctgttc tctactgctt catggactga ttttccttgc
      361 gtttatctgg cgtttttccg aagttcagcc cgctatgtct cctgcaccgg cgatcatgct
      421 gcaatgggca gaaattatag aggctccttc ttctccactt tcgcttccgg tgggaatcgc
      481 tcaacaggag tccgcagtga cagaagaaaa gcaacaaact gaggacagac aacaacgtcc
      541 tgtcactgaa gacagtgatg caaccattga aataaccagg aagaaaaagt catccgatgg
      601 agaaaagaga aagacacgtc cacctcgtaa gataaaggct caaaccagcg acagcaaccc
      661 cacagccgtt tccagtaatg ctgcacctca ggctctggtc gaatcgtccc gtattgctgc
      721 gccttttaac agtgactcaa caaagcgaga taattctgag gcatcttggg aaagcagagt
      781 aaaaggccac cttaaccgtt ataaacgtta ccctggcgat gccagaaaac gtgcacgaac
      841 cggcacagcc gtcgtgagtt ttaccgtaaa cacggaaggc actattgtca gtagtttctt
      901 ggaaatctct tcaggaaccc tttctcttga ccgcgaagct attgctgttt tagaacgcgc
      961 tcaaccactt cccaaaccac cgcctgaaat attggagggg ggattattta aagtaaaaat
     1021 gccgattacc tttaagttga aggagtaaag ggataattgg ccgttcaagt tcatttaact
     1081 gaaatttaat tttcattttt atgctgaaca ttttatcaaa cttttccact gtcgaaaaaa
     1141 tcaaaaagac agtagatcat aattaataaa caaatagtta tacttatatt tattaatatt
     1201 atatctaagg aaatttatga acgttataaa actggctatc ggctcaggca tattattgct
     1261 cagctgcggt gcttactcac aatccatcag tgaaaaaact aattccgaca aaaaaggagc
     1321 ggcagaattc agtccgctca gcgtttctgt cgggaagacg accagtgagc aggaagctct
     1381 cgagaaaaca ggcgcgacca gttcccggac aacggacaaa aacctgcaat cacttgacgc
     1441 aacagtgcgt agtatgcccg gtacttatac tcaaatagat cctggtcagg gagcaatcag
     1501 tgtgaatatt cgaggcatga gcggatttgg tcgtgtaaac actatggtcg atggtattac
     1561 ccagagtttt tacggaacct ctacctccgg aacaacgacg catggttcaa ctaacaatat
     1621 ggctggcgta cttatagatc ctaacttact ggtagcagtt gatgttacac gcggtgacag
     1681 cagtggctct gaagggatca acgcccttgc cggtagtgta aatatgcgta ctattggcgt
     1741 tgacgatgta atatttaacg gtaatacata tggccttcgt tcacgtttct ctgtcggtag
     1801 taatgggctg ggacgcagcg gaatgatcgc ccttggtgga aaaagcgacg cttttacgga
     1861 tacgggaagc attggcgtta tggctgctgt gagcggcagt tctgtgtact ctaatttctc
     1921 aaatggttct ggaattaaca gcaaagagtt tggttatgat aaatatatga agcagaaccc
     1981 caaatcccaa ctgtataaaa tggatatcag accagacgaa tttaacagct tcgaactttc
     2041 cgctcgaacc tatgaaaata aatttacacg tcgtgatata accagtgacg actattacat
     2101 taaatatcat tacacccctt tttctgaatt aattgacttt aacgtaacgg ccagtaccag
     2161 tcgcggtaat caaaagtatc gtgatggctc gctgtatact ttctacaaaa cctcagcgca
     2221 aaatcgttct gacgcgctgg atatcaacaa taccagccgg ttcactgtcg cggacaatga
     2281 cctggagttt atgctgggca gcaaactgat gcgtacccgc tatgaccgga ccattcactc
     2341 agcggcgggc gacccgaaag cgaatcagga atcgatcgag aacaatccgt tcgcaccctc
     2401 cggccagcag gatatttcag cgctgtatac cgggctgaag gttacgcgcg gcatctggga
     2461 ggcagatttc aatctcaact acacacgtaa caggatcaca gggcacaagc ccgcctgcga
     2521 ttcacgcgtt atctgcgtgc cacagggtag ctacgatatt gacgataaag tgggtggctt
     2581 caacccttca gttcagcttt ctgctcaggt aacaccatgg cttcagccgt tcattggcta
     2641 cagcaaatcc atgcgcgccc cgaacatcca ggagatgttc ttctctaatt caggaggcgc
     2701 atccatgaac ccattcctga agcctgaacg tgcagaaacc tggcaggcgg gttttaacat
     2761 tgataccaga gatttagtgg tcgaacagga tgccctgcgc tttaaggctc tggcgtaccg
     2821 cagcaggatc cagaactaca tctacagcga gtcttatctg gtttgttctg gaggtcgtaa
     2881 atgcagtatg gctgaggtga ttggcaatga ctgggagggc attagcgatg aatacagcga
     2941 caatatgtac atctacgtta actcggcaag cgacgttatc gcaaagggct tcgaactgga
     3001 gatggattat gatgcaggtt ttgcttttgg ccgactctct ttcagccagc agcaaacaga
     3061 ccagccaacc tccatcgcca gcacctactt tggcgcaggg gatatgaccg aactgcccag
     3121 aaaatacatg acgctggata ctggtgttcg cttcttcgat aacgcgttga ccctgggcac
     3181 tatcataaaa tacacaggca aggctcgtcg cctgtcgcct gattttgagc aggacgaaca
     3241 taccggcgca ataatcaaac aggatttgcc gcagatccca acgattatcg atctctatgg
     3301 tacttacgag tacaaccgca acctgacact gaaactttcg gtacaaaacc tgatgaacag
     3361 agattattcg gaggcgctga ataagctcaa catgatgcca ggtcttggtg acgagaccca
     3421 cccagccaat tccgcgcgtg gcagaacatg gatatttggc ggggacattc gtttctgatg
     3481 acaatttccg ctaggttcag acaatacgtt ttctctctta tgtcaatttt attgcaggaa
     3541 cgaaaaatga atattttcac tttatccaaa acaccgctat acctgttaat ttcactattt
     3601 ttacccacga tggccatggc tatcgatcca cctgaacgcg aactttcgcg atttgccctg
     3661 aaaacgaatt accttcagtc ccctgatgaa ggcgtctatg aactggcgtt tgataatgcc
     3721 agtaaaaagg tgtttgcagc agtcaccgat cgtgtaaatc gtgaagccaa taaaggctat
     3781 ctgtattcgt ttaattcaga ttcgctgaaa gtcgaaaata aatacacgat gccataccgg
     3841 gcattttcgc tggcgataaa tcaggataaa catcagctct atatcggaca cacccagtca
     3901 gcgtccctgc gtatcagtat gtttgacacc ccaaccggca aactggtaag aaccagcgac
     3961 aggttaagtt ttaaagcggc aaacgctgca gattcgcgtt ttgagcattt gcgccatatg
     4021 gtttacagcc aggattccga taccctgttt gtgagttata gcaatatgct gaaaacggcc
     4081 gagggcatga agcctctgca taagctgtta atgctcgacg ggacgacgct tgccttaaaa
     4141 ggcgaggtta aggatgctta caaaggtaca gcgtatggtc tgacgatgga tgaaaaaaca
     4201 cagaaaatct acgttggcgg aagagattac atcaacgaaa ttgatgcgaa aaatcagacg
     4261 ctgctgcgta ccatcccgtt gaaagatccg agaccacaaa tcacaagtgt gcagaatctg
     4321 gcggtggact ccgcttctga ccgtgccttt gtggtggtat tcgaccatga cgatcgttcc
     4381 ggtacaaaag atggactcta tatttttgac ttacgcgacg gtaaacagct tggctatgtg
     4441 cacacaggag ccggagctaa cgcggtgaaa tacaatccga aatataacga actgtatgtc
     4501 accaacttca ctagcggcac catcagcgta gtggatgcca ccaaatacag catcacccgt
     4561 gaatttaaca tgccggtcta cccaaaccag atggtgttgt cgggtgatat ggataccctt
     4621 tacattggca tcaaagaagg ctttaaccgc gattgggatc ctgatgtgtt tgtggaagga
     4681 gctaaagaac gtattctgag cattgatttg aaaaagtcgt gatcggggga aaacatggga
     4741 taaaaactcc gttattccac taaactggcc ggactaggaa aagcaaacat ctctgtcctg
     4801 ccggtacgcc tgttacgccg ctccatgagg ctgaggaatc tccagaaaac gagacaggca
     4861 cagcattgta cgatcaactg atttgtgcca ataattcaat gaaatcacag ctatgcctgt
     4921 tcatggggtt ctaggccgga accccagatt tttccgattg tttgttttct aactgattat
     4981 tcagtagttt tagagggctg acgctcagtg gaacgaaaac tcacgttaag aggttttgac
     5041 tatgataaaa gtcaaagtag gggtgagttt ggatatggaa attattgtac tataagggct
     5101 tccccgaaca gtcaacgtcg gtatttatgg tttgtctgca ctacagttaa aaataaaggg
     5161 gagagactgc ggtactataa acggtcataa taaatcgtta tcgattcgga ccctgatgaa
     5221 agacgcgcgt gaggctcttg ttcgttaagc ggatacgtat atcctgtaca ataatcagat
     5281 atacctcacg gggtctaacc ctttatcatc aacgagttgc aggtcagata gtcatacggt
     5341 tgctgcgtgc tttagctggc tgaatacagt ctgaaatcat caccgtaatg caatgatgcc
     5401 cagcacagcc ttgcattttt ggcggcgatg gccacaacag cgcgccagta tcctctgcgt
     5461 tcgaccagtg aacagaccca acgactgaat gagtcagtcc ttttctcagc gccaatcagt
     5521 actgaacgag caccctgaac cagaagcgtt cgcagatacg aatcaccggc ttttgttatc
     5581 ctgcctagct tcgactttcc gccgctgctg tattgtgatg gagtcagtcc cagccaggcc
     5641 gccagttgcc gtccattttt aaaatcatgt gcattaccga tacaggcaac cagcgcacag
     5701 gcagttgtgg gaccaattcc ttttagctcc atcagccgct gactgcggtg atctgttctg
     5761 gccatacggg acaaaatttt gtcatattca gtgatgttag cttcaatgcg gggtcagttt
     5821 ggagatcgta aaattttgtt cgttaagcac attatttaac caatagatat gcgtaacgga
     5881 ttgattttgc gtcaacggct atcgctttta agttatcgcc attgaggtat tgcatttagg
     5941 ttatccgata gaggtcaaat cctctgaaaa cgccgtatag cacgggagta caccctaccg
     6001 caatagcgca accgtacaat attgtacgtt tcccaaacgc agcctaggaa gcacgtcgtc
     6061 agtattggag aaaacttaca aacgctcgcc gcagccccag cgaccgaacg ttagtgagcg
     6121 agtgggcgag gaagcggaaa agtgtcaggt ctgaattgag cacatacgcc agaaattata
     6181 tagcaaaaag atatatgatt tttacattgc tcagtagatt gtaaaatgct gctgattgga
     6241 tatctatatc taatttaaaa taatggcttt aagatggtca gaaaccatct tccagttata
     6301 ttctcctgtt gcagcattca aagcgataat ttgcatttca tcaaacgaaa tacgcgaact
     6361 aataatatgt ataccattcc gttttaaaaa aacctgtgca acaaacaagg ctgttcgctt
     6421 atttgcatcg ttgaaaatgt gaccacgagc aatagcaact aaatacacgg cagccagatc
     6481 cataatatct gtcacacctt cataatgata catgttcaaa acacgatgaa tgatcgcgtc
     6541 agcgcgaccg ggttctggca tacccgccac gccaccatca cgcgaaatca agcggtcatg
     6601 aaactcaatc atttcctgtg aactaacgaa tttcatcgct tagaaagctc cgtataggcc
     6661 gtcccatgac tttgcatgat ctcagcaaac tcagcatcaa gagccgcttt acggtatcgt
     6721 tcaaaagcgt ctttatcaac cagtacagcc gcgccacctt tacggcgctg aatctcaaca
     6781 ggctgatttt catcaaccgc cagctcgagc aacgaggcca ggttatcgcg agcatggcta
     6841 tagttacatg taaacatagt gcttcctctt tgagttgtac aattaaattg tacaacagtt
     6901 atcttcaata tgaaatcacc gttaccgctg gcgcatcctg acccaactta accggggaga
     6961 aagtcgccat gccgttgcca gagctatctg tcacggtcag aaaggtgaga taagaaagcg
     7021 atataccgat ggtcaagaag atcaactggg cgcactgggg ctggtgacta acgccgtcgt
     7081 attatggaac actatttata tgcaggctgc cctggaccat ttcagggcac agggggaaat
     7141 cgtgaatgat gaagatatcg cacgcctctc accactttgc cacggacaca tcaatatgct
     7201 cggccatatt ccttcacgct ggcagaactg gtgactaaag ggcaaaaaga ggcatcggag
     7261 gaagaaaaca ttgcttaacg tgagttttcg ttccactgag cgccagcccc ctaattctct
     7321 atatccaaac tgaccccaat aggtgacggg ttatgtgacg aaatctgatg tagaaatcgt
     7381 tgtttcagtg acagtcactc aatcggtcat ttatgtgaca ccccacgccg ttactggtcg
     7441 cggaaaaatc cagcggaaaa atccaggttt ccggcttggg accccatctc aagagatcct
     7501 ttgatctttt ctactgaacc gcgatccccg tcagttcaga agacgaagat gacgcaacgg
     7561 ttcctcattg tacatcacga atatagtttg cttgacatcc tgacaagaat aatattttaa
     7621 ttagaaaaat tattattttc atatcccttt ccgacaaaag taaaaatact tatgcattta
     7681 aaatactacc tacata
//
LOCUS       DAZGAN020000174         7593 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGAN020000174 DAZGAN020000000
VERSION     DAZGAN020000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.174"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            99..560
                     /gene="yehR"
                     /locus_tag="ACHKYO_RS23300"
                     /old_locus_tag="pgaptmp_004660"
     CDS             99..560
                     /gene="yehR"
                     /locus_tag="ACHKYO_RS23300"
                     /old_locus_tag="pgaptmp_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HGU4431359.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(600..1070)
                     /gene="yehS"
                     /locus_tag="ACHKYO_RS23305"
                     /old_locus_tag="pgaptmp_004661"
     CDS             complement(600..1070)
                     /gene="yehS"
                     /locus_tag="ACHKYO_RS23305"
                     /old_locus_tag="pgaptmp_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HGU4431360.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(1117..1836)
                     /gene="btsR"
                     /locus_tag="ACHKYO_RS23310"
                     /old_locus_tag="pgaptmp_004662"
                     /gene_synonym="yehT"
     CDS             complement(1117..1836)
                     /gene="btsR"
                     /locus_tag="ACHKYO_RS23310"
                     /old_locus_tag="pgaptmp_004662"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HGU4431361.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1833..3518)
                     /gene="btsS"
                     /locus_tag="ACHKYO_RS23315"
                     /old_locus_tag="pgaptmp_004663"
     CDS             complement(1833..3518)
                     /gene="btsS"
                     /locus_tag="ACHKYO_RS23315"
                     /old_locus_tag="pgaptmp_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HGU4431362.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3740..3805
                     /locus_tag="ACHKYO_RS23320"
                     /old_locus_tag="pgaptmp_004664"
                     /pseudo
     CDS             3740..3805
                     /locus_tag="ACHKYO_RS23320"
                     /old_locus_tag="pgaptmp_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4033..4281
                     /locus_tag="ACHKYO_RS23325"
                     /old_locus_tag="pgaptmp_004665"
     CDS             4033..4281
                     /locus_tag="ACHKYO_RS23325"
                     /old_locus_tag="pgaptmp_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4431363.1"
                     /translation="MRVEICIAKEKISKMPKGSVSALQQEMLRRVSKRYDDVDVIVRA
                     ANNDSLTILRAVDKESAHEFVQETLQEAWETADDWFIR"
     gene            complement(4318..4491)
                     /locus_tag="ACHKYO_RS23330"
                     /old_locus_tag="pgaptmp_004666"
     CDS             complement(4318..4491)
                     /locus_tag="ACHKYO_RS23330"
                     /old_locus_tag="pgaptmp_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431364.1"
                     /translation="MLPDLQRLQVIQGQMMNKDKCRIYGRKRYTAGAMCFMLFVWLGG
                     VVTLCAVAAIIMQ"
     gene            complement(4496..4969)
                     /locus_tag="ACHKYO_RS23335"
                     /old_locus_tag="pgaptmp_004667"
     CDS             complement(4496..4969)
                     /locus_tag="ACHKYO_RS23335"
                     /old_locus_tag="pgaptmp_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431365.1"
                     /translation="MKVILATRNRYLEYGLQALLKEHSVILAREFFLPENRRYIPDFD
                     ESWLIICDTLLGRLMRCMFQGRHFLQLGAESLRDGEQISEAIRNGVWTYNSVARPLTM
                     SEMVVMFGYVYRQSRPCRLASEMGIHTKTVNTFLYTGMAKNGLYGVSVKQIACAE"
     gene            complement(5058..6332)
                     /locus_tag="ACHKYO_RS23340"
                     /old_locus_tag="pgaptmp_004668"
     CDS             complement(5058..6332)
                     /locus_tag="ACHKYO_RS23340"
                     /old_locus_tag="pgaptmp_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HGU4431366.1"
                     /translation="MSKKFTKTILSSAVAGLLLVSGGVVAANPEDVNIGDEYIVKFYK
                     DGEGAKIVKAKGNEEVLGAINTTVGSVMTWNDQDAKKVQEFIKTLPKEQQQVGTQIAM
                     QRIVFPVDSPSLKLDKLKSGTLTKADIDAVVSLKKDVSNAISIKTAPAYVSAVRSGGN
                     SDAALAAAKQGPGVLQEYNRIVTNIDRLNKDTTFAIDANGNITLDETGSVERESVKNV
                     VAAIQADTTIYQEEDGSYTHDVTAPKNVRVSDAIVDLDKSIKDRTTVGVSSDGTLTRA
                     EGAKNTISVNDGLVALSGRTDRIDAAVGAIDGRVTRNTQSIEKNSKAIAANTRTLQQH
                     SARLDSQQRQINENHKEMKRAAAQSAALTGLFQPYSVGKFNATAAVGGYSDQQALAVG
                     VGYRFNEQTAAKAGVAFSDGDASWNVGVNFEF"
     gene            complement(7139..>7593)
                     /locus_tag="ACHKYO_RS23345"
                     /old_locus_tag="pgaptmp_004669"
     CDS             complement(7139..>7593)
                     /locus_tag="ACHKYO_RS23345"
                     /old_locus_tag="pgaptmp_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HGU4431367.1"
                     /translation="DTEENRKADISGRWGFDGEKITDLLTAEKARGMKGDEINAWRNA
                     MEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFWTDADNNDVPMDAETL
                     IALSAAAEQAMFTKGLEIHIRQRTMKKEIEALGDAEAILAYKVGMADR"
BASE COUNT         1981 a         1977 c         1615 g         2020 t
ORIGIN      
        1 ctccagttcc ggactgctca cgtcacggta ttaggcatat tctatatagc ccctggtgag
       61 agtcaccagt ttcttgatta aataaaatgg agttttacat gaaggctttc aataagctgt
      121 tttccctcgt tgttgcatct gttctggttt tctctcttgc tggctgcggt gacaaagaag
      181 aatcgaagaa attcagcgcc aatctgaacg gcactgaaat tgccattacc tatgtctaca
      241 aaggtgacaa ggtgcttaag caatcttctg aaaccaaaat tcaatttgct tctattggtg
      301 caaccaccaa agaagacgct gccaagacac ttgagccgtt aagcgccaaa tacaaaaaca
      361 tcgcgggtgt tgaagaaaaa ttaacctata cagataccta cgcgcaggaa aacgtgacta
      421 tcgatatgga aaaagtggat tttaaagccc tgcagggtat ttcaggaatc aacgtttctg
      481 ctgaagatgc caaaaaaggt atcactatgg cgcaaatgga actggtgatg aaagccgctg
      541 gttttaaaga agtgaaataa tcggttggcg gtcatgctct aaacatgacc gccaattttt
      601 tagccttttt tcacatgctg gcgcgctgcc agtccacgca gaaaataacg taaaaattga
      661 tcgccgcatt cgcggaagtt tttatgatcc ggtgcacgca tcatcgctgt aatttccggc
      721 atcgaaacgc ggaactgctg ttcggtgagg atcgccagaa tgtcatcggt tttcagcgaa
      781 aacgcgatgc gtaatttttt cagcacgatg ttgttattaa tgcgacgttc cggctccagt
      841 gccggagcag actcatcctt gccgcgtttt tcataaatca ggccattgag gaatgacgac
      901 aaaacaatgt ccggacaacg ctgaaaaccc tcttcgtctt ctttacgtag ccagacggcg
      961 atctgttccg cggtggcttc gacattaccc agcgccagaa tacgcaccag gtcattatta
     1021 ttggctttca aaatgtagcg cacgctgcgc agaatatcgt tacttagcat gaggccttca
     1081 ggtgttgatg aggcaaaaag ccattttagc agtcttttac aggccaatcg cctcttttaa
     1141 gcttttcaga taacggcggc tgaccggcac ggttaagcca ttacgcaaaa tcaactcggc
     1201 ctggccgtta tcttccagac gaatctcctg taaatgcgcg aggttaacca gatactgacg
     1261 atggcagcgc agtagtggtg tacgactttc cagggtacgt aatgtcaatt cggtaaagcc
     1321 ctctttccct tcgtggctgg taacgtagac accgctcatc cgactgctga caaatgccac
     1381 atctttcatt tgcagcaaat aaatccgact atgccccgta caagggataa atttcagcgc
     1441 ctgttgattt tccggtaaca gcgaaacatc ctgcttgctg cgttcctgac gcaaacgcgc
     1501 cagcgttttc tccagtcgcg cttcatcaat tggcttcagc agataatcaa aggcatgttc
     1561 ttcaaaggct ttaatagcgt attcgtcaaa cgcggtgaga aaaacaatat acggacgatg
     1621 ttccgggtcg agcatcccca ccatttccag accactgatg cgcggcatct ggatatcgag
     1681 aaacagcaca tccgggcgca gtttatgcac cgcgccgatc ccttccacgg cgtttgaaca
     1741 ctctccaacg atttcaatat cgctctgctc ctgcaaaaat acacgcaggt tctcccgtgc
     1801 taacggttca tcatcgacaa ttaagacttt aatcatgcct cgtccctcca tggtagtcgt
     1861 aacgttattc gggtgtaact atcaggctca caggcgacgc ttattccata gtcatcgcca
     1921 aaccgttcac gtaaacgctt atccaccaga ttcatcccca gcccactggc attggttacc
     1981 ggttgataca aaccggcatt gtcttcgatc tccagcatca aatgttgccc ctcacgtcgg
     2041 gcgctgattg ccactcgccc tgtatccagc agttgtgatg tcccatgttt aatggcgttt
     2101 tccactatcg gttgcagggt aaacgcgggc aattgctgct gggataattc ttgcggaata
     2161 gcaatgttga cctgcaaccg cgactggaag cgcgcctttt caatttgcag ataagcgttc
     2221 acatgttcaa tttcgtcggc gagagtaaca aactccgaag gccgctttaa gtttttgcgg
     2281 aaaaaagtgg aaagatactg caccagctgg ctggcctgtt cgctgtcgcg gcggatcacc
     2341 gctttaatgg tgttaagcgc attaaacaaa aaatgaggat tcacctgggc gtgaagcagt
     2401 ttgatttctg actgggtgag catcgctttt tgccgctcat attgcccggc aagaatctgc
     2461 gccgaaagca gttgcgcaat cccctcgccc agcgtgcggt ttattgaact gaataaacgg
     2521 tttttggctt catacaattt gatggtgccc atcacgcgct gattttcgcc acgcaacgga
     2581 attaccagcg tcgaccccag tttgcattgc ggatgcaaag agcaacgata aggtacttcg
     2641 ttgccatcag cgtagaccac ttcaccggtt tcaatcgctt ttaaggtgta agtcgaagaa
     2701 atcggtttgc cgggtaaatg gtggtcgtca ccaattccgg taaaggccag caatttctct
     2761 cgatcggtaa tcgcgactgc accaatatcc agctcctgat acagcacctg agccactttc
     2821 atgctgttca cttcgttaaa cccctgtcgc aaaatgcctt ccgtcgaggc tgccactttc
     2881 agcgcagtgg cagaaaaagc agaagtgtat ttttcaaaca tcgcgcgttt atcgagcaat
     2941 atacgcataa acagcgccgc gccgacggta ttggtgacca tcattggtgc agcaatatta
     3001 ctcaccagac gcaccgcatc ttcataaggt cgggcgatcg caaggatgat cagcatttgc
     3061 accatttcag cgacgaacgt gacggcaccg gcggtaatgg ggttaaagac tttatcagtg
     3121 cgcccgcgac ggatcaggat gctgtgtacc aggccgccga gcaatccttc aacgatggtt
     3181 gagatcatgc aacttagcgc ggtcatgccc cccatcgaat atcgatgtaa gccgccggtc
     3241 agaccaacca gcccaccgac gaccggaccg ccgagtaagc cgcccattac cgcgcctatc
     3301 gcacgggtat tggcaataga atcgtcaatg tgcaacccaa accaggtgcc catgatgcag
     3361 aagatggaaa agacgatgta acagagaaat ttatgcggca gacgaaccgt gacctgcatt
     3421 aacggtatga ataatggcgt tttactcatt aaccacgcaa tgactaaaaa aacgcacatc
     3481 tgctgaagca gcagcaacac cagattaaaa tcgtacatac tcgcaaacca cacttccctt
     3541 taaaacgcgt aacatacatt gcctgcgttt aactttcttt gaactcttgc agaaaaatga
     3601 gaattcgtga gtacgatcac tcaaaatcgc ctggcaaaaa taaaatcacc ctatagatgc
     3661 acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa accgggacct
     3721 cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt tgctttgtga
     3781 tattaaccct gtcacgttac gcgcgtgacg caacattttt aaaaatcgac gaagatcgtc
     3841 caggagcgcc gtttttcaag ggttggatag atgattcgtt taccatgtcg acgaacttcg
     3901 acgaatttcg ccgataaaaa gtgtcccata catgccccat tgatgcccca taaacaatat
     3961 caggaattga cactggttat ccatacagac aaaaataaca cctgtacata accacagtta
     4021 caggagggtg ttatgcgtgt tgagatctgt atcgctaaag aaaaaatcag caaaatgcca
     4081 aaaggttctg tttctgccct gcagcaagaa atgcttcgac gtgtcagtaa acgttatgac
     4141 gatgtggacg tgattgtcag agccgccaac aatgacagtc tcaccatttt aagagctgta
     4201 gataaagaat ccgcgcacga atttgttcag gaaacactgc aggaagcctg ggaaaccgct
     4261 gacgactggt ttattcggta aatcttcttt acggggagtt aacttactcc ccatttttta
     4321 ttgcatgata attgcagcaa cggcacagag tgtcaccaca ccacccaacc acacaaacag
     4381 catgaagcac attgctcccg ctgtataacg ttttctcccg taaatacggc atttgtcttt
     4441 attcatcatc tgcccctgaa tcacctgaag acgctgcagg tccggtaaca ctgaatcact
     4501 ccgcacaggc aatctgcttt acgctcacac catacagccc gtttttcgcc attcccgtat
     4561 acagaaaggt attcaccgtc ttcgtgtgaa tacccatttc actggcaagg cgacagggtc
     4621 ttgactgacg gtagacgtag ccaaacatca ccaccatctc tgacatcgtc agcggacggg
     4681 caacgctgtt atacgtccat acaccattgc gtatggcttc acttatctgc tcaccatcac
     4741 ggagtgattc cgcacccagt tgcagaaaat gccgtccctg gaacatgcag cgcatcagtc
     4801 tgcccagcaa cgtatcacag attatcagcc aggattcgtc aaagtccggg atataacggc
     4861 ggttctccgg caggaaaaac tccctcgcca gtatcacact gtgttccttc agtaacgcct
     4921 gcagaccata ctccagatag cgattcctgg tagccagaat caccttcatc gcagcacctt
     4981 cctgtttttt atgccccatg aaaaacggca gaccagagtc ctgcgcctgc cgtcatactt
     5041 catgtcacct tcagcaatta aaactcgaag ttcacaccaa cgttccagga ggcatcgcca
     5101 tcactgaatg ccacgcctgc tttcgctgcg gtctgctcgt tgaagcggta acccacaccc
     5161 actgccagag cctgctggtc gctgtaacca ccaaccgctg ccgtggcgtt gaatttaccc
     5221 acactgtacg gctggaacag acctgtcagg gccgcagact gtgctgctgc tcgtttcatt
     5281 tccttatggt tttcattaat ctggcgctgc tggctgtcca gacgtgctga atgttgctgc
     5341 agcgtacggg tattggctgc aatcgccttg ctgtttttct caatggattg cgtgttacgg
     5401 gtgacacggc catcaatggc accaactgct gcatcaatac ggtcagtacg accagacagg
     5461 gccaccagac catcgttaac agaaatggtg ttctttgcac cttctgcacg ggtcagggtg
     5521 ccatcagaac tcacaccaac agtggtgcga tctttgatgg atttatccag atcaacaata
     5581 gcatcgctta cacgcacatt ttttggagct gtaacatcat gtgtatagct gccatcctct
     5641 tcctgataaa tggttgtatc tgcctgaatg gctgcaacta cattttttac agactctcgc
     5701 tccactgagc ctgtttcatc aagggtaatg ttaccgttag catcgatagc aaaagttgtg
     5761 tctttattca acctatctat attggtaacg atgcggttgt attcctgcag aacacctggg
     5821 ccttgtttag cagcagcaag cgcggcatca gagtttcccc ctgaacgaac cgcagacaca
     5881 tacgctggcg ctgttttgat agaaatagca tttgaaacat ctttttttaa ggatacaaca
     5941 gcatcaatat ccgccttggt taatgttcct gacttcaact tatcaagctt taaactagga
     6001 gaatcaaccg ggaacacaat tcgttgcatc gcaatttgtg taccaacttg ttgttgttct
     6061 tttggcaatg ttttaatgaa ttcttgaact tttttagcat cctgatcgtt ccaagtcatg
     6121 acactaccta cagttgtatt tatagcacct aaaacctctt catttccttt tgcttttact
     6181 atcttggcac cttccccatc cttgtaaaat tttacgatgt actcgtcccc aatatttacg
     6241 tcctcaggat ttgccgcaac aaccccacca ctcaccaaca acagaccggc aacagcagat
     6301 gaaagaatag tctttgtaaa tttcttgctc atgtggtata ccctataaaa ttatctgtat
     6361 acaaataaaa cagttcaaca tcggatatat aaagaaaatc tgatgtctcg ccgctctcat
     6421 gagcttactc ctgaagaaat tccgaatttt aatataaagt ttttcggaat ttccttacta
     6481 taacgtcggt gatatcacgt ccgcgaaggg atatccgcat tacgactggc ctgccactta
     6541 tgctgtctct ttattcatca acaatttgta tacggttaca tctttaattg tgaagttagt
     6601 cacaaaaaga agaaagtcaa ataatcaaat tgtgcttaac tgtttacatc agcacaatct
     6661 gatgaccatt gaatgaccat tgaaggacgc actaaattat tttcagcaat ccctcagcct
     6721 acgaaaattc tgtatttgat gattgtttta tttgctctat ttttatgcag tcattctgtt
     6781 gcttataaaa aagcaccact atctcatgta taatttctgg gcctgtcgtc tccccgtttt
     6841 tcacttacag atgccgggca acattcagtt tcaggaatca gcagttacca tgcaatacga
     6901 aaattctgta ttgcatccgc gccctcatct caatatcttt gtcgccggat aaaaataact
     6961 atcaaccaca agtatcatgc tctcggggag cacgcactcc ccagtttttt atctttgaca
     7021 ggggtgaagg aggccgttgc atctccttta attatcattg ccagattatt tcctcgacct
     7081 gagtattctt tatttagcca tagctttgta gcaaggggct ggagcgcccc tctttttttc
     7141 acctgtcagc catgccgacc ttatacgcca gtatagcttc cgcatcaccc agcgcttcaa
     7201 tctctttttt catcgtgcgc tgtctgatat gaatttccag acctttggta aacatcgcct
     7261 gctctgcagc agcactcaga gcaatcagcg tttcagcgtc catcggtacg tcattattgt
     7321 ccgcatccgt ccagaaaaac gcctccggca gtttccccgc tttcgctgcc gccaccgacg
     7381 gctcaagacg cgtctgcgtt gacttcccgt agtcccattt acgcccattg tgctcaaacg
     7441 tgtagttcgc cgcttccatc gcattacgcc aggcgttaat ttcatcgccc ttcatccctc
     7501 gcgctttctc cgcggtcagc aggtccgtga ttttctcccc gtcaaaaccc cagcgccccg
     7561 agatgtcagc tttgcggttt tcttccgtat ctg
//
LOCUS       DAZGAN020000175         7556 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGAN020000175 DAZGAN020000000
VERSION     DAZGAN020000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.175"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..364
                     /locus_tag="ACHKYO_RS23350"
                     /old_locus_tag="pgaptmp_004670"
     CDS             <1..364
                     /locus_tag="ACHKYO_RS23350"
                     /old_locus_tag="pgaptmp_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431368.1"
                     /translation="IAKDFCRPGEEVSSSIASKVFHAFIDLVNKIRGKKDFMYICTLC
                     CFAEEVKGDYSHYRTFLFDIGNQYKVKLTQSGKKELSLTLEFNDTIIESQKVTGNKAK
                     HILEDIEKFYRNKPDTYY"
     gene            complement(586..1779)
                     /gene="tyrB"
                     /locus_tag="ACHKYO_RS23355"
                     /old_locus_tag="pgaptmp_004671"
     CDS             complement(586..1779)
                     /gene="tyrB"
                     /locus_tag="ACHKYO_RS23355"
                     /old_locus_tag="pgaptmp_004671"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HGU4431369.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHIAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(2032..3111)
                     /gene="alr"
                     /locus_tag="ACHKYO_RS23360"
                     /old_locus_tag="pgaptmp_004672"
     CDS             complement(2032..3111)
                     /gene="alr"
                     /locus_tag="ACHKYO_RS23360"
                     /old_locus_tag="pgaptmp_004672"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGU4431370.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(3164..4579)
                     /gene="dnaB"
                     /locus_tag="ACHKYO_RS23365"
                     /old_locus_tag="pgaptmp_004673"
     CDS             complement(3164..4579)
                     /gene="dnaB"
                     /locus_tag="ACHKYO_RS23365"
                     /old_locus_tag="pgaptmp_004673"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU4431371.1"
                     /translation="MAGNKPFNRQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4662..5645
                     /locus_tag="ACHKYO_RS23370"
                     /old_locus_tag="pgaptmp_004674"
     CDS             4662..5645
                     /locus_tag="ACHKYO_RS23370"
                     /old_locus_tag="pgaptmp_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HGU4431372.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDYLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5811..6053)
                     /gene="pspG"
                     /locus_tag="ACHKYO_RS23375"
                     /old_locus_tag="pgaptmp_004675"
     CDS             complement(5811..6053)
                     /gene="pspG"
                     /locus_tag="ACHKYO_RS23375"
                     /old_locus_tag="pgaptmp_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HGU4431373.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRDDRWRY"
     gene            complement(6187..7224)
                     /gene="dusA"
                     /locus_tag="ACHKYO_RS23380"
                     /old_locus_tag="pgaptmp_004676"
     CDS             complement(6187..7224)
                     /gene="dusA"
                     /locus_tag="ACHKYO_RS23380"
                     /old_locus_tag="pgaptmp_004676"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HGU4431374.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
BASE COUNT         1963 a         1949 c         1830 g         1814 t
ORIGIN      
        1 aattgcaaaa gatttttgta gacctggaga agaagtatca agttctattg caagtaaagt
       61 ttttcacgca tttattgatt tagttaacaa aatcaggggt aagaaagact ttatgtatat
      121 ttgcacgctt tgctgttttg ccgaggaggt taaaggtgat tattctcatt acaggacctt
      181 tttatttgat attggcaatc aatataaggt taaacttaca caaagcggaa aaaaagagct
      241 ctctttaact ttagaattta acgatactat aattgaatct cagaaagtca caggcaataa
      301 ggcaaagcat attcttgagg atatagaaaa attctatcgt aacaaacccg atacttatta
      361 ttaaatttaa taatagttag atgtgcagaa attattactc cacccaaaaa aacaggataa
      421 ggaattccag ataattagga taagcttaac aataaaagaa ctaataacgt ttattatctc
      481 tcacgtagaa cgatggcatc aaaaaaatca tctataaaga aagagtgaaa gcgacgacag
      541 tttaatttca ctgcaggctg ggtagctcca gcctgctttc ctgcattaca tcaccgcagc
      601 aaacgccttt gccacacgtt gtacatttgc cgtatttaac ccggcgacac acatgcgacc
      661 gctggcgatg agatagacac caaattcttc acgtagtcgg tcaacctgag cggcacttaa
      721 accggtataa ctgaacatgc cgcgctgatt aagcagataa tcgaaattgc gttctggcat
      781 ctctgtgctt aataccttca ccagttcctg acgcattgcc agaatgcgag tacgcatctc
      841 ttctacttcc gccagccagc tggctttcaa tgcctcgtca ttcagcactg cagccaccac
      901 ctgcgcacca aaattcggcg ggctggagta gttgcggcga actgttgctt tcaattgccc
      961 cagtacgcgg ccagcggctt cggcatcttc acacataaca gaaagtccgc cgacgcgctc
     1021 gccgtaaagg gagaaaattt tcgagaacga attgctcacc agagcgggta atccagcgct
     1081 ggcaatggcg cgaatagcgt aggcatcctc ttccataccg gcaccaaatc cttgataggc
     1141 aatatcgagg aatggaataa gctcgcgggc tttgagaatt tcaatcaccg catcccactg
     1201 gtcattagtg agatcggcac ccgttgggtt gtggcaacat ggatgcagca acacaatact
     1261 gcgggcaggt aatgttttca gcgtcgccaa caggtcatta aagcgcacgc cgttagtcgc
     1321 ttcgtcatac caggggtaag tactcacttc gaatccagcc ccggcgaata ttgctatgtg
     1381 gttttcccag gtaggatcgc tgacccagac gcctgattcc gggaagtagc gtttcaggaa
     1441 atccgcgccc actttcaatg ccccggagcc gccaagggtt tgaatggttg ctacgcgctg
     1501 ttgtttcagt accggatggt ccgcaccaaa cagcagcggc gcaatggcat ggcgatagct
     1561 gttaagccct tccatcggta aataaagcga agcgccatga ggctgcgcat tcaggcgcgc
     1621 ttccgcctcc gccacggctt tcagttgtgg aataattccg tcttcgttgt agtacagacc
     1681 gatacttaaa ttcactttgt cgctgcgagg gtcttcttta aaacgctcca taagcgtaag
     1741 aatcgggtcg ccagcgtagg cgtcaacttt ttgaaacacg cgatggttct ccaggtttac
     1801 gggcaggtgg ttaaaacaca ataaaccgga agaaggcgaa gatcgagtgg atgttcagga
     1861 gcgaacggca attagcaaca gagtgagact catgacaaac gtacatccgc cagaggcacg
     1921 acctttataa gagcatgaaa gaacatcaac ttattgaatt tttaggattt tattggccgg
     1981 ataaggcatt cacgccgcat ccggcacaga caatcaaata ttacagaact attaatccac
     2041 gtatttcatc gcgacccttg aagtcaggcg cgtaataagt tcgtaagcgc ttactttcgt
     2101 catttcagcg atacgttcta cgggcaaacc ttcgccccat aaaatgaccg gatccccggc
     2161 tttgtcctgc gcctgtggac ctaagtctac gcagatcata tccatcgcca cgcgcccgac
     2221 aatcggtact tcgcgaccgt tcaccagcac tggcgtaccg gacggcgcgg cgcgcggata
     2281 accatcgcca tagcccatcg cgactacgcc aagacgggta tcacgttcgc ttacccaggt
     2341 tccaccataa ccaacaggtt ctccggcttt atgctcacgc acggcaatca ggctggaggt
     2401 tagtgacatc actggctgac agccaaaatc ggcaccggtg gagcgatctt ccagcggcga
     2461 gacgccataa agaatgatgc ccgggcgcac ccagtcaaaa tgcgactgtg gccacagcag
     2521 aatgccaccc gatgcggcaa tggaacgttg accaggtttg ccttcgcaaa aggtattaaa
     2581 gatagcgagt tgtttctcgg ttgcgccaca ttttggttca tccgcgcgcg caaaatggct
     2641 gacgatattc accggctgac gaacgttttt gcactgggtc aggcgatgat aaaacgcctc
     2701 agcctgttcc ggccttacgc ccagacggtg cataccggta tcgagtttca tccagacggt
     2761 aaccggctcg tccaggctag cctcttccag cgcagccagc tgttcttcgt tatgcacggc
     2821 ggtatgaaaa tgttgcgcag aaatcgtcgg cagatctctg gcatcaaaaa agccttcgag
     2881 taacagtaca ggtttggtga ttccccccgc acgcagtcgc agagcttctt cgagacgggc
     2941 tacgccaaag gcgtcagcat cggggagcgt tcgcgcggtc tcaagaagac cgtgaccata
     3001 agcgttcgct ttcaccaccg caaccatttt actggcaggt gccagttcac gaagacgttg
     3061 caggttgtgt cgcagagcgc ggcggttaat cacaacagtt gccgcttgca tttgtgttcc
     3121 ttgataagtg tttgctttaa ttacctaatt cataaaataa ttattattcg tcgtcgtact
     3181 gcggccccgc atagttgtcg aagcgcgacc attgaccgtt aaaggtcagg cgtaccgtcc
     3241 cgattgggcc gttacgttgt ttaccgataa taatttccgc gatgcctttt aaatcactgt
     3301 tttcgtgata cacctcatca cgatagataa acatgatcaa gtccgcatcc tgctcgatag
     3361 agccagattc acgcaggtcg gagttgaccg ggcgtttgtc ggcacgttgt tccagagaac
     3421 ggttcaactg ggacagcgcc accaccggca cgttcagttc tttcgccagt gctttcagcg
     3481 agcgagagat ttctgcaatt tccagcgtac ggttatcgga aagcgccggt acgcgcatca
     3541 gttgcaggta gtcgatcatg ataagcccga tgccgccgtg ttcacgggca atacggcgtg
     3601 cgcgggaacg cacttccgtt ggcgtcaggc cggaggagtc atcgatatag atattgcgtt
     3661 tttcgagcaa aatacccatg gtgccggaaa tgcgcgccca gtcttcatca tcgagctgcc
     3721 cggtacggat tttagtctgg tcaacgcgcg acagcgacgc cagagaacgc atcataatct
     3781 gttctgaagg catctccagc gagaagataa gtactggttt atcctgcaac atcgccgcgt
     3841 tttcgacgag gttcatcgca aatgttgttt tacccatcga cggacgcgcg gcgacgatga
     3901 tcaaatccga cggctgcaag ccagcggttt ttttgttgag atcgtcataa ccggtgttta
     3961 ctccggtaac gccatcgtgt ggctgctgaa acaactgctc aatacgcgcc acggttgcgt
     4021 cgagcacatc ggcgatgttc ttcggccctt cgtctttgtt tgcacgactt tcagcaattt
     4081 taaagacgcg ggattcagca aggtccagca gatcttcgct ggtgcgcccc tgcggatcaa
     4141 aaccggcttc agcaatctca ttcgcaaccg aaatcatctc acggacaacg gcacgttcac
     4201 gcacgatgtc cgcataggca ctgatgttcg ccgcacttgg cgtatttttt gacagctctg
     4261 ccagataagc aaaaccaccg acgctatcga gttgcccctg gcgttccagc gattccgcaa
     4321 gagtaattag atcgataggg ctaccgcttt cctgcaaacg cgccatttca gtaaagatat
     4381 gacggtgtgg gcgggtgtaa aaatcgtctg ctaccacacg ctcggctaca tcatcccagc
     4441 gttcgttatc tagcattaaa ccgcccaaca ccgactgctc cgcttcgatc gagtgcggag
     4501 gcactttcag cccggcaact tgtggatcgc gttcgcgggg ttcagcctgc tgtctgttga
     4561 agggtttatt tcctgccata gtgaatggag ttaccgagat aaagaatggg tcgaaacttt
     4621 accatatgaa gcagaccctg acgatacgtt ctggaggaca catggcaaca cgaattgaat
     4681 ttcacaagca cggtggcccg gaagtacttc aagccgtaga gttcactcct gccgatccgg
     4741 cagagaatga aatccaggtc gaaaataaag ccatcggcat caattttatc gacacatata
     4801 tccgcagcgg cctttacccg ccgccatcgc tacccagcgg attaggcacc gaagcagcag
     4861 gcatcgtgag taaagtcggc agtggtgtaa agcatattaa ggcaggcgat cgtgtagtct
     4921 atgcgcagtc ggcgttaggc gcttacagct ctgtgcataa cattattgcg gataaagcgg
     4981 cgattctgcc tgcggcaatt tcttttgagc aagctgcggc atccttcctg aaaggcttaa
     5041 cggtttatta tctgctgcgc aaaacctatg aaattaaacc cgatgagcag ttcctgttcc
     5101 acgcagcagc tggcggcgtt ggcttgattg cctgccagtg ggcaaaagcc ctgggcgcga
     5161 aacttatcgg caccgtagga accgcgcaaa aagcgcagag cgcgctaaaa gcgggcgcgt
     5221 ggcaggttat taactatcgt gaagagaatc tggtcgagcg gttaaaagag atcaccggcg
     5281 gtaagaaagt gcgcgtggtg tacgattccg tgggcagaga cacctgggaa cggtcgctgg
     5341 attacctgca acgccgcggc ttaatggtga gttttggcaa ctcatcaggt gcggttaccg
     5401 gtgtgaactt aggcattctc aatcagaaag gctcgttgta tgtgacacgc ccttccctgc
     5461 aaggctatat caccacgcgg gaggaattaa ccgaggccag taatgaactg ttctctttga
     5521 ttgccagcgg tgtgattaag gtcgatgtcg ccgagcagca gaaatatccg ctgaaggatg
     5581 cgcagcgtgc gcatgaaatt ctggaaagcc gggcaacgca aggttccagc ctgctgattc
     5641 cataaaagaa atagggcttc cacctgggaa gccctttctt tttatagttc ggctgtatgt
     5701 agggtacagc acgatgaatc tgttagaggc gcaatagtga cagatttgat tatcaattcc
     5761 tattttgttc taaggataaa accttaggtt gtgatcatcc gcacaatccc ttagtaacgc
     5821 cagcggtcat cacgctgata tttcggcatt tttggtgctt taatcgcctt aataacccac
     5881 accaccgcaa tcgccagtag taaccacggc agcaacttaa tcatcaatgc cagcataccg
     5941 ccgaggaaca taatggccgt cgccacaacc agcgcggcga taatgcccag caacgaaacg
     6001 ccggtgacca tcagcatgac aaaaaagcca atcacaaaaa gtagttccag catgatgctc
     6061 tcccaaatat gaaatctctt gctggcatta caagaatcat gccaaaaata atctattgat
     6121 ttaacagcaa aacgccccgc gacggtgcgc agggcgtggt gaatttgact actttttggt
     6181 gaaaagttaa cgcttatccg ccaccagttt gagcgcgtgt tccagcacgt taatgtcagc
     6241 acccgcttta tgggcatttt cacttaaata acgccgccac tgccgcgcgc caggaatacc
     6301 ctggaacaaa cccagcatat gccgggtaat atggccgaga tacgtcccct ggctgagttc
     6361 acgctcaatg tacggataca tggcgcgcac taccgccacc ggatcggcat cggtatccga
     6421 ggaaccaaag atctcccggt ctaccgccgc cagaataccc ggattctgat acgcctcgcg
     6481 cccgaccatc acgccatcca tatgttgcag gtgtgctttg gcctcttcca gcgacttgat
     6541 accaccgtta atcgacattg tcagatgcgg aaagtcacgc ttcagttgat acacacgcgg
     6601 ataatcgagc ggcgggattt cacggttttc tttcgggctt aaccccgaaa gccaggcttt
     6661 acgtgcgtgg atgataaaca tctcacactc gcctttgccg gaaacagtgt tgatgaaatc
     6721 gcagagaaat tcatagctgt cctggtcatc gatgccaata cgcgttttca ccgtcactgg
     6781 aatcgacacc acatcgcgca tcgctttcac acagtcggca accagctgcg cattacccat
     6841 cagacacgca ccaaacatgc cgttctgcac ccggtcagac gggcagccga cattcaggtt
     6901 gatctcatca tatccacgcg cttctgccag cttcgcacac tgtgccagcg ccgccggatc
     6961 gctaccgccg agttgcaacg ctaccggatg ttcttcttca ctgtacgcca ggtaatcacc
     7021 tttaccgtga ataatcgccc ctgtggtcac catttcggta tacagcaacg tattgcggga
     7081 aagcagacgc aagaaatagc ggcaatgtct gtccgtccag tcgagcattg gtgcaacgct
     7141 aaaccgacca ctccagtgaa cgtcagtttt ttcaggcatt gcgctggttt ggttgatttt
     7201 ttgcatttca gaattaccgt gcattttcaa atgtagagat tattttatcg atatatcatt
     7261 gggttatgtt attcagcatc actgttcagg aggctcaata gcggggtact ataccataac
     7321 aacaggaagc gcctgtctca ttgcaaaaga aaattgagat caaatcaagg catgaagctc
     7381 tcacgaagtg atggaaataa tcttattagc cgttagcctt gttaaggaca atgataaaca
     7441 atccaggttc gacgataaat aaaaaatcac acattaaact ccggtgatat ctcttcctgc
     7501 taatggcact gatagaagaa aaaagaaccc aataagtatt aggttctttt atgtaa
//
LOCUS       DAZGAN020000176         7432 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGAN020000176 DAZGAN020000000
VERSION     DAZGAN020000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.176"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(166..528)
                     /locus_tag="ACHKYO_RS23385"
                     /old_locus_tag="pgaptmp_004677"
     CDS             complement(166..528)
                     /locus_tag="ACHKYO_RS23385"
                     /old_locus_tag="pgaptmp_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HGU4431375.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            813..1022
                     /gene="yibT"
                     /locus_tag="ACHKYO_RS23390"
                     /old_locus_tag="pgaptmp_004678"
     CDS             813..1022
                     /gene="yibT"
                     /locus_tag="ACHKYO_RS23390"
                     /old_locus_tag="pgaptmp_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HGU4431376.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(1034..1621)
                     /gene="mtlR"
                     /locus_tag="ACHKYO_RS23395"
                     /old_locus_tag="pgaptmp_004679"
     CDS             complement(1034..1621)
                     /gene="mtlR"
                     /locus_tag="ACHKYO_RS23395"
                     /old_locus_tag="pgaptmp_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HGU4431377.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(1621..2769)
                     /gene="mtlD"
                     /locus_tag="ACHKYO_RS23400"
                     /old_locus_tag="pgaptmp_004680"
     CDS             complement(1621..2769)
                     /gene="mtlD"
                     /locus_tag="ACHKYO_RS23400"
                     /old_locus_tag="pgaptmp_004680"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU4431378.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            complement(2999..4912)
                     /gene="mtlA"
                     /locus_tag="ACHKYO_RS23405"
                     /old_locus_tag="pgaptmp_004681"
     CDS             complement(2999..4912)
                     /gene="mtlA"
                     /locus_tag="ACHKYO_RS23405"
                     /old_locus_tag="pgaptmp_004681"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HGU4431379.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            5449..5811
                     /locus_tag="ACHKYO_RS23410"
                     /old_locus_tag="pgaptmp_004682"
     CDS             5449..5811
                     /locus_tag="ACHKYO_RS23410"
                     /old_locus_tag="pgaptmp_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU4431380.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5814..6950
                     /locus_tag="ACHKYO_RS23415"
                     /old_locus_tag="pgaptmp_004683"
     CDS             5814..6950
                     /locus_tag="ACHKYO_RS23415"
                     /old_locus_tag="pgaptmp_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4431381.1"
                     /translation="MDLLIVLTYVALAWTVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT         1817 a         1867 c         1844 g         1904 t
ORIGIN      
        1 gctatttcgg taaaaatgca ttttcagaat atcaaccagc ggtaaatcga cgtaaaggga
       61 gtgaattgtt tggtaattag gcgagtagac aggagagtcc ccgcattgac cagctaaggc
      121 aagccagatg cgggttgata tgatcgaacc agtcgtctga ggcgcttaaa acgcagtctt
      181 agagaatcca gtcatctcct gcaggcccat ttcacggccc agcgcggtca ttgggtgcac
      241 aaccaccagt ccgcgaacac ttttcttcag cttgcccata tcagcctgct cttttttggt
      301 aattgcgcgc tgaaacggca tcttcatcag cttctgtgct tctttactca gtttttggct
      361 atgaacttca cgcaggcgag caatttctgc ttccagcgtg gctttctctt tttccagctc
      421 ggcatatttg tctgcggctt caaccagcga cagatcggcc tgctggtggc ggatggcgtc
      481 cagacgatcg ctgagacgtt tgatttcgtt tttttcgact tctttcatgt gatggcttct
      541 gataaagata aatttatagc ccctagtatg cgcgaccagg tgctttgttc acaactgatg
      601 acattgcaat agttctgtaa cgtaatcatt aacatgctta aaaactacgc aatttattga
      661 ttaattaaga aatctataga aaaaagataa taaacagcag cggatgatta ttgaggattc
      721 agcacgtttt ccttaatgtc atctttaccg ggaacctaaa ctaaaattgg cggccgcgca
      781 ttcccggcgc ggggtaatac ggagatatca tcatgggcaa attaggtgaa aacgttccgc
      841 ttcttatcga taaagccgta gatttcatgg catcaagcca ggcgttccgg gagtatctga
      901 aaaaacttcc tccccgtaac gcgattccgt ccggaatacc cgatgaaagc gtgccgttat
      961 atctacaacg tctggagtat tatcgtcggc tttatcggcc gaagcaggta gaggggcagt
     1021 aagcagacgt tgcttacttt tgaaaggctt tttttaagct gattctggaa atcagctcag
     1081 tcagtgaaag gaccattgtt gatcgcacag cctgttgata gcgctgaatt tgcattgcat
     1141 acaaactgga gtctgctggt tcaaactgtg gcggcggtgg taacgccgcc acgcagtgca
     1201 gttcaccaaa gggtccaagg atctcgtcgt cggtaaaggc gtactcgttg ccgtcgtgat
     1261 ttagttcttc acgcaatgcc atcagcagct ccgcatcttc gtattcctgg cggttaatga
     1321 cgcccaaccc gtaaatgagt tttaaacgca cagaaagatc gcccagcgga ccgtcgccgt
     1381 cgagtaacgg ttctacagca tacttcaccg cgtaatcgtc tttgcggaat acctgaagca
     1441 ccagaagatt taccgcctcg gtcaaaagct cgacggcggt gatcagaaag cttcgcacgg
     1501 ttttgccagc attcagacgc tcaagcacac ggttttcaaa ggcctgggtt tgttccattg
     1561 ttgcctgcat atctgacaat ctgttattcg ggcgcagggt gtcctgcgcc tggtccacca
     1621 ttattgcatt gctttataag cggttaccgc ctcggataca acctcgctgt tggcatcaag
     1681 accggaaatc tgtgccagcg ccgcctgcgg acctttgtca gcgatcagtg ctgccagttc
     1741 ctgagcctgc ggatcatctt cactgcggaa gtgcattgca ccagcaatcc cctgaatcag
     1801 attcttgtgc ggaaggctgt attccagcgt accgagcaat ggcttgatca gacggtcgcc
     1861 agcactcagt ttacgcagcg gctgacggcc tacgcgctct acatcatctt tcagatacgg
     1921 gttctcaaaa cgaccgagga ttttctggat gtacgccgca tgtttgtctg cgtcaaagcc
     1981 gtagcgcttg atcagtaccg caccactttc ttccatcgca ccttttacca ccgcgcggat
     2041 tttctcgtcg agaatagcgt cacgaatggt ctgatgaccg gccagttttc cgaggtacgc
     2101 ggttatagca tgacccgtgt tcagggtgaa gagtttacgt tcgacaaatg ccatcaggtt
     2161 gtcggttaac tccatgcctg ggatgttcgg cagtgcgcct ttgaactgcg ttttatcgac
     2221 aatccattcg ctgaaagttt ctaccgtcac ttccagcgga tcgttagttg ccgaagccga
     2281 aggcggtacg atgcggtcaa cggcggaatc gacaaagcca acgtgttctt ctacccacgc
     2341 tttggcgtct tccggcaggg cgttcatcac atggcctttc agctgcgtgg taccgcgtac
     2401 catgttttca caggcgatga tgttcaacgg ggattcatta ccttgttctt tgcgtttcac
     2461 cagccctttg gcgatagccg gagcaatacg ttccagcaca accgggccaa cggcggtagt
     2521 gactaaatcg acctgagcaa tcagatcaac gacatcatca ccaatgctgc tgacagcatt
     2581 gacgccggaa acggtatcta cctgctcggt ttcaccaacc acatgaacct gatagctatg
     2641 acgggcattc agggcatcaa gtaccacctg attgacatcg gcaaacgtca gttggatacc
     2701 cgcgtctgcc agcagtttac cgataaagcc acgaccgata ttacctgcgc caaaatgtaa
     2761 tgctttcata gtattaacct tcatcaatgt ttttacccga gagggctggg gtgaggcttt
     2821 tccctcaccc aaaccctctc cgtgaggaga ggggcagcgc cggatgcggc gtaagcgcct
     2881 tatccggcct acaagaacgt gcaaattcaa caaatcgtga tgcatcctgt aggcctgata
     2941 agcgtagcgc atcaggcaat taaccccacc ttctccatgt ggagagggtg ggattggatt
     3001 acttacgacc tgccaacagt tccagcactt catccacgct ggtggtgtgt gccagacgct
     3061 cgatgacgga ctcatcatcc agtgcattgg tcaggctggt gataacctga atgtgctcgt
     3121 tgttacgggc tgcaatacca atcaccagac gggcaatgtc atcttcttct tcaccgaagc
     3181 gcacgccttc cgggtactgg cagaacacca cgcccgtttt cagtacgcga tctttcgctt
     3241 caaccgtacc gtgtggcacc gcgatagact cacccagata agtcggggtc agtttttcac
     3301 gatccagcat cgcctgaacg tattccggct caacgtaacc gcctttcacc agctgctcgc
     3361 cagcaaaacg aatcgcttct tctttggttg ccgctttgcg accgaggaag atgttctccg
     3421 cgcctagctt gaacaggtta gcactggaat cgtcaaagct gtctttcagg ctgtctttta
     3481 ctttctcttc gtttgccgtg tggcgttggg cagcaaccag acgttcggtc aggctggtgt
     3541 acaggccgct gtcgaggaag ttggtcagcg aaatatgctg tgcctgcgga acctggcgca
     3601 tagcgcgttc ggtcaggtca cggtgagtga tgacgaggtc cacatctggc ggcaggttgt
     3661 tgatcgcgct gttagtaaca gaaatctgcg acagacctgc atcctgaatt ttcttacgca
     3721 gaacgcctgc gcccatcgca ctggaaccca taccggcgtc acaggcaacg atgattttac
     3781 gtacgtggct caggtcgtta gtcacatcgc cagcagacag cggagatgcg cctttagact
     3841 cagctttcat gtcctgcata cgacgagttg ctgcttcaat atcatcttct tctttcactt
     3901 tgctggtttt cagcaaaata gcagagacaa cgaaggagac agccatcgcc gcacacacac
     3961 ccgcgatgtt agcgaagtaa gcaccttttg gcgtcatcgc cagtaccgca aggatagacc
     4021 ccggagatgc tggagaaacc agaccaccgc ccaggatagt cagcgtgaac acgccagtca
     4081 taccgccgag gataacggcc aggatcagac gcggattcat cagcacatac gggaagtaga
     4141 tttcgtggat accacccagg aagtggatga ttgccgcacc gcccgcagac tgtttagcac
     4201 taccacgacc aaagaacatg tacgccagca gcacgcccat acctggacct gggttagctt
     4261 caatcaggaa gaagatggat ttacccagtt catgggactg ctgaataccc agcggcgaga
     4321 agataccgtg gttaatggcg ttgttgagga acaggatttt cgccggttca acaaagatag
     4381 acgccagcgg cagcatgtca tggacaacca tgaagttaac gcccgcagcc agcattttgg
     4441 acagggcttc aacaatcggg ccaatgccga gaaatgccag aatggcgagg atcatcccga
     4501 tgatgcctgc ggagaagtta ttcaccagca tctcaaaacc ggatttgatc ttaccgtcta
     4561 cccagcggtc gaagtgctta atgcaccagc cgcccagcgg acctgcaatc atagaaccga
     4621 ggaacatcgg catgtctgcg ccgacgataa cgcccatggt agtgatggca ccgactacgc
     4681 cgccacgttc gccgcctacc agcttaccac cggtataacc gatcagcagc ggcaggagat
     4741 aagtgatcat cggcccgacc agcttcgcca gcgtctcgtt cggtaaccac cctgttggaa
     4801 taaataacgc ggtgatgata ccccacgcga taaacgcgcc gatatttggc atcaccatgt
     4861 tgctgaggaa acgaccaaag ctttgcactt tgatcttaat atcggatgac ataaaaacac
     4921 cccttcttat gtttgctgtc gcgcaggctg gaagcccgag gttttttgtt aatgtggcgg
     4981 cagaggtagc cggaccttgt agatactgcg aaatctggca ctgaatcggt taactgtcca
     5041 gtcgacggcc ttttgtgtga tatcagtcac ataaatatag ggggcagaga ggtaattgac
     5101 gtgatactca tcacaaaacg cttgtgtaaa aaagcaacaa gacggcgatt ttcgctaaaa
     5161 atggcattta aatgtgagta gtgtcacatt tttgttttga tggtttgttg aatctttgtg
     5221 atctgaatca caagatattt tcttacggcg ttcacctgat gtgatctaca gcatgttatg
     5281 ccttcgtcat acaatcgtta tgtaactaag caactcatca cctctaaaaa ttattctttg
     5341 gtgttatggc gagaatcgcg aacccggatt attgtcaaaa tatcagcctg actcccttct
     5401 ggctgccagc ccaaaaacaa cactgataaa cgtgaatccg aggcagatat gtttctaaac
     5461 tatttcgcgt tgggagtgct gatattcgtc tttctggtga ttttttatgg aatcatcgcg
     5521 atacatgaca tcccttatct gattgccaaa aagcgcaatc acccccatgc cgacgctatt
     5581 catacggcgg gctgggtgag cctgtttact ctgcatgtta tctggccgtt tctgtggatc
     5641 tgggcgacgc tctatcaacc ggagcgtggc tggggtatgc agtcacatgt tgcgtcgcag
     5701 gagaaagcga ctgacccgga aatcgcctca ctttctgacc gaatttcccg gctggagcat
     5761 caactcgccg ccgagaaaaa gactgactat tccacgttcc cggagatcta attatggatc
     5821 tattgattgt tttaacttac gtggcgctgg cgtggacggt ctttaaaatc ttccgcattc
     5881 cggtaaatca gtggacgctg gcgacggcgg cgctgggagg cgtgtttctg gtgagtggtt
     5941 taattttgtt gatgaactac aaccaccctt acacttttac cgcgcaaaag gcagtgatag
     6001 cgatccctat cacgccacag gtgacgggaa ttgttactga agtcactgac aagaataatc
     6061 agcttattca aaagggcgag gtgcttttta agctcgaccc ggttcgttac caggcgcgag
     6121 ttgacagact tcaggctgac ctgatgacgg cgacgcataa tataaagacg ctgcgtgcgc
     6181 agctcactga agcgcaggcc aacaccaccc aggtttcagc ggagcgcgac cgtctgttta
     6241 aaaattatca acgttacttg aaaggcagcc aggcggcggt gaatccgttc tcggaacgtg
     6301 acatcgacga tgcgcggcaa aatttcctcg cgcaggatgc gctggtgaaa ggctcggtgg
     6361 cggagcaggc gcagatccag agccagctcg acagtatggt taacggcgag caatcgcaga
     6421 ttgtgagctt aagagcgcaa cttactgaag caaaatataa ccttgagcag actgtcattc
     6481 gcgcgccgag caatggctac gttactcagg tactgatccg cccaggtaca tacgcagctg
     6541 ccttgccgct gcgtccggtg atggtcttca tccccgagca aaaacggcaa attgtcgccc
     6601 aatttcggca aaactcgctg ttacgtctga aacccggcga tgatgcggaa gtggtgttta
     6661 acgcgctacc tgggcaggtg tttcacggca aactgactag tattttacct gtcgtgccag
     6721 gcggttctta tcaggcgcag ggggtattgc aatcattaac ggtcgtgccc ggcacggacg
     6781 gtgtgctggg aaccattgaa ctggacccta acgatgatat cgatgcctta cccgacggca
     6841 tctacgccca ggtggcggtt tactccgacc atttcagcca tgtttcggtg atgcggaaag
     6901 tgctgctaag aatgaccagc tggatgcatt atctttattt ggatcattga gggggagggg
     6961 ggagattatg gaactttgct ccttgtaagg actaattatt tttgcatatt acttcattag
     7021 ctgcgatgat ttttctttaa tattcattat gttaataagt tctggatgag tgtgctctca
     7081 ttggattttt tatccgtctt gttctgccgc aggatgagac tatacatcgt cgagatcgca
     7141 atgatgatat gagttgttcc tcctgcgacg gctctaatca aaaaagagca ggagtgcttt
     7201 tccatctatt gcgatgatgg ctttattatc tgaaggatga cagtcacatt taaaaaatat
     7261 ttaatctata gatcacaatg tagggtcatg gaatatatgt atacaactat tttcttcttc
     7321 acttttaatt atcgtattca ttataaaact aagttaaaaa aactcctgga gagtgagtta
     7381 ttaatatatt tttaaataaa attaagttat cattcatcga tgcactttat ag
//
LOCUS       DAZGAN020000177         7260 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGAN020000177 DAZGAN020000000
VERSION     DAZGAN020000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.177"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            138..914
                     /gene="exuR"
                     /locus_tag="ACHKYO_RS23420"
                     /old_locus_tag="pgaptmp_004684"
     CDS             138..914
                     /gene="exuR"
                     /locus_tag="ACHKYO_RS23420"
                     /old_locus_tag="pgaptmp_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HGU4431382.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1154..1237
                     /gene="yqiM"
                     /locus_tag="ACHKYO_RS23425"
                     /old_locus_tag="pgaptmp_004685"
     CDS             1154..1237
                     /gene="yqiM"
                     /locus_tag="ACHKYO_RS23425"
                     /old_locus_tag="pgaptmp_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HGU4431383.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1259..1921
                     /gene="yqjA"
                     /locus_tag="ACHKYO_RS23430"
                     /old_locus_tag="pgaptmp_004686"
     CDS             1259..1921
                     /gene="yqjA"
                     /locus_tag="ACHKYO_RS23430"
                     /old_locus_tag="pgaptmp_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HGU4431384.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1925..2308
                     /gene="mzrA"
                     /locus_tag="ACHKYO_RS23435"
                     /old_locus_tag="pgaptmp_004687"
     CDS             1925..2308
                     /gene="mzrA"
                     /locus_tag="ACHKYO_RS23435"
                     /old_locus_tag="pgaptmp_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HGU4431385.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2455..2823
                     /gene="yqjC"
                     /locus_tag="ACHKYO_RS23440"
                     /old_locus_tag="pgaptmp_004688"
     CDS             2455..2823
                     /gene="yqjC"
                     /locus_tag="ACHKYO_RS23440"
                     /old_locus_tag="pgaptmp_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HGU4431386.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2861..3166
                     /gene="yqjD"
                     /locus_tag="ACHKYO_RS23445"
                     /old_locus_tag="pgaptmp_004689"
     CDS             2861..3166
                     /gene="yqjD"
                     /locus_tag="ACHKYO_RS23445"
                     /old_locus_tag="pgaptmp_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HGU4431387.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3169..3573
                     /gene="yqjE"
                     /locus_tag="ACHKYO_RS23450"
                     /old_locus_tag="pgaptmp_004690"
     CDS             3169..3573
                     /gene="yqjE"
                     /locus_tag="ACHKYO_RS23450"
                     /old_locus_tag="pgaptmp_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU4431388.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3563..3862
                     /gene="yqjK"
                     /locus_tag="ACHKYO_RS23455"
                     /old_locus_tag="pgaptmp_004691"
     CDS             3563..3862
                     /gene="yqjK"
                     /locus_tag="ACHKYO_RS23455"
                     /old_locus_tag="pgaptmp_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HGU4431389.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4048..4440
                     /gene="yqjF"
                     /locus_tag="ACHKYO_RS23460"
                     /old_locus_tag="pgaptmp_004692"
     CDS             4048..4440
                     /gene="yqjF"
                     /locus_tag="ACHKYO_RS23460"
                     /old_locus_tag="pgaptmp_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU4431390.1"
                     /translation="MKKLEGVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4510..5496
                     /gene="yqjG"
                     /locus_tag="ACHKYO_RS23465"
                     /old_locus_tag="pgaptmp_004693"
     CDS             4510..5496
                     /gene="yqjG"
                     /locus_tag="ACHKYO_RS23465"
                     /old_locus_tag="pgaptmp_004693"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HGU4431391.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5789..6154
                     /gene="yhaH"
                     /locus_tag="ACHKYO_RS23470"
                     /old_locus_tag="pgaptmp_004694"
     CDS             5789..6154
                     /gene="yhaH"
                     /locus_tag="ACHKYO_RS23470"
                     /old_locus_tag="pgaptmp_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4431392.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6396..6752
                     /locus_tag="ACHKYO_RS23475"
                     /old_locus_tag="pgaptmp_004695"
     CDS             6396..6752
                     /locus_tag="ACHKYO_RS23475"
                     /old_locus_tag="pgaptmp_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4431393.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(6803..>7260)
                     /gene="yhaJ"
                     /locus_tag="ACHKYO_RS23480"
                     /old_locus_tag="pgaptmp_004696"
     CDS             complement(6803..>7260)
                     /gene="yhaJ"
                     /locus_tag="ACHKYO_RS23480"
                     /old_locus_tag="pgaptmp_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGU4431394.1"
                     /translation="IVIAPDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSE
                     VTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMV
                     EKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFSGK"
BASE COUNT         1835 a         1771 c         1765 g         1889 t
ORIGIN      
        1 ttttttagga atttataaat attcacaata ttaattcaat acattaattc aatcaccaaa
       61 tttatatatg accaatggca tacccatgaa taaagtggta tgataacttc gtccgcattc
      121 cactccggga gacgcatatg gaaatcactg aaccacgccg tttgtatcaa caacttgccg
      181 ctgacctgaa agagcgcatc gaacagggcg tctatctggt aggtgataaa ctgcctgcag
      241 aacgctttat tgccgatgaa aagaacgtca gccgtacggt agttcgtgaa gccatcatca
      301 tgctggaagt tgaaggctat gtcgaagtgc gtaaaggttc aggtattcat gtggtttcca
      361 accagccgcg ccatcagcag gcggctgaca ataatatgga attcgccaat tacggtccgt
      421 ttgagttgct tcaggctcgc cagctcatcg aaagtaatat tgccgagttc gcggcaactc
      481 aggtaacgaa acaagacatc atgaaactga tggccattca ggaacaggcg cgcggcgaac
      541 aatgcttccg tgattccgag tgggatttac agttccacat tcaggtagcc ctggcgacgc
      601 agaactccgc cctggcggcc atcgttgaaa aaatgtggac ccaacgtagc cataacccgt
      661 actggaaaaa actgcacgaa cacattgatt cccgtaccgt cgataactgg tgtgatgacc
      721 acgatcaaat cctcaaggcg ctgattcgca aagatcctca tgctgctaag ctggcaatgt
      781 ggcagcacct ggaaaacacc aagatcatgt tattcaacga aaccagcgac gacttcgagt
      841 tcaatgccga ccgctatctg ttcgccgaaa acccggtagt gcatctcgat acggccacca
      901 gcggcagtaa atgaaatacc tgtccgacag gcgctgcgac gcgcctgttt gtctcgtcgg
      961 taagcgaaaa gtataaagtg tcagcctgtg taaatcctct cgccaccctc ccctgcattc
     1021 agcaaaatca gactccacgg acatgtaatt ttgataacga acaacgttga cctttgttac
     1081 aattagattc aatttgaatt tatgtttttg aatgctttct tatctcacga tttaacaggg
     1141 aatagttcag gctgtgttga tgtatcaaac ccgcagaaca taccaaaaca gcaataacat
     1201 tgcggtagtg catcttttaa aaccagcgtg gcgttaaccg attcaccagg aataatgaat
     1261 ggaacttttg acccaattgc tgcaagccct gtgggcgcag gattttgaaa ccctggccaa
     1321 tccatcgatg attggcatgt tgtattttgt cttgtttgta attttgttcc ttgaaaacgg
     1381 cttgcttcca gcggcctttt taccgggcga cagtttactg gtattggtcg gcgtgttgat
     1441 tgcgaaaggc gcgatgggtt acccgcaaac gattctgctg ctgaccgttg ccgccagcct
     1501 cggctgctgg gtcagctata ttcaggggcg atggctgggc aatacccgca ccgtacaaaa
     1561 ctggctatct catttacccg cgcattatca tcaacgcgca caccatcttt ttcataaaca
     1621 cggtttatcg gcgctgttaa ttggtcgctt tattgcgttt gtcagaacac tgctgccgac
     1681 gattgccggg ttatcagggc tgaataacgc gcgctttcag tttttcaact ggatgagcgg
     1741 tctgctgtgg gtattgatcc tgacaactct gggttacatg ctcggcaaaa cgccggtatt
     1801 tttaaagtac gaggaccagc tgatgtcatg cctgatgctg ctcccggtgg tgctgctggt
     1861 gtttggcctg gcaggttctc tggtcgtgtt atggaaaaag aaatatggaa atcgggggta
     1921 agggatgcaa atacctcgca tgtcgcttcg ccagctagcc tggtccggca ctgttttact
     1981 tctggtcggc acgctgttgc tggcctggtc cgcggttcgc cagcaagagt ctacgctggc
     2041 gattcgtgcc gttcatcaag gcacaacgat gccagacggt ttttcgatct ggcatcacct
     2101 tgacgctcat ggcattcctt tcaaaagtat cacccccaaa aacgacactc tgttaattac
     2161 atttgactcc agcgaccaga gcgccgccgc aaaagcggtc ctcgacagaa cattgcccca
     2221 tggctacatc attgcgcagc aggacaataa cagtcaggct atgcagtggc tgacccggtt
     2281 acgggataac tctcatcgct tcggataact tcctgttatc cgaaacatat cactcacttt
     2341 ggtgatttca ccgtaactgt ctatgattaa tgaggcggtg gttaacacta ccgcttcgtt
     2401 gtctggacaa tcgttccttt gtaataggtc cgaatcacaa tggaaggttc aagaatgaaa
     2461 taccgcatcg ctttagctgt ttctctcttt gctcttagtg ccggtagtta tgccactacc
     2521 ctgtgtcagg aaaaggagca aaatatcctt aaggagatca gctatgccga aaaacaccaa
     2581 aaccagaatc gtattgacgg tctgaataaa gccctgagtg aagtccgggc caactgttca
     2641 gatagccagc tgcgtgccga tcatcacaag aaaatcgcaa agcagaaaga tgaggtggcg
     2701 gaacgccagc aagatttagc cgaggcgaag caaaaaggcg atgccgataa gattgccaaa
     2761 cgcgaacgga aactggcaga agcgcaggaa gagctgaaaa agctggaagc gcgcgactac
     2821 taactcacaa tagtcactac ttactcacct ggagaaaact atgtcgaaag aacacactac
     2881 ggaacatctg cgtgctgagt tgaaatccct ttccgatacg ctggaagagg tgcttagctc
     2941 atctggcgag aagtcgaaag aagagttgag taagattcgt agcaaagcgg agcaggcact
     3001 gaaacagagc cgttatcgcc tgggtgaaac cggtgatgcc atcgccaaac aaacccgtgt
     3061 cgcggcggcg cgtgctgatg agtatgtgcg tgaaaatccg tggaccggcg tgggcattgg
     3121 cgctgcaatc ggtgtagtgc tcggcgttct gctgtcgcgt cgttaattat ggcggacact
     3181 catcacgcgc aagggcccgg taaaagcgtt ctgggcatcg ggcagcgaat tgtttccatc
     3241 atggttgaaa tggtggaaac acgtctgcgg ctggcggtgg tggagctgga agaggaaaaa
     3301 gctaatctct ttcaactttt actgatgctg ggcctgacga tgcttttcgc tgcatttggt
     3361 cttatgagcc tgatggtgct aattatttgg gcggttgatc cgcaatatcg cctgaatgcg
     3421 atgattgcca ccaccgtggt gttgctgcta ctggcactga ttggcggtat ctggacgtta
     3481 cgtaaatcgc gtaaatccac cttgctgcgc catacacgcc atgagctggc aaacgatcgg
     3541 caactgctcg aggaggagtc ccgtgagcag taaagtcgaa cgtgaacgac gtaaggcgca
     3601 actgcttagc cagatccagc aacaacggct ggatctttcc gccagtcgtc gtgaatggct
     3661 ggaggcaaca ggcgcttacg atcgtcgctg gaatatgctg ctaagtctgc gctcctgggc
     3721 gctggttggc agtagcgtga tggcgatctg gacgattcgc catcctaata tgctggtccg
     3781 ctgggccaga cgcggttttg gcgtatggag cgcctggcgt ctggttaaaa cgaccctcaa
     3841 gcagcaacag cttcgcggtt aacttccctc tggccggagc aattccggcc ttatccctca
     3901 aattttttga agatttttga cagttttcct tgctaacaat catcattcac cacgtttatg
     3961 attctctcca tcgacagcaa cgacgctaat accgcgccct tgcacaaaaa aacaatcagc
     4021 agcctgagtg gcgcagtgga gagtatgatg aaaaaattag aaggtgttgg tgtactggta
     4081 gcgcgcattt taatgccgat tctgtttatt accgcaggct ggggaaaaat caccgcttac
     4141 tctggtaccc aacaatatat ggaagcaatg ggtgtcccgg gttttatgct gccgctggtg
     4201 attctgcttg agttcggtgg tggtctggca atcctgttcg gtttcctgac tcgcaccaca
     4261 gccctgttta ctgcgggctt tacgctgctg acggcatttt tatttcacag caactttgct
     4321 gaaggcgtca actcgctgat gttcatgaaa aacctgacaa tttctggcgg attcctgctg
     4381 ctggcaatta ccggtccggg cgcgtatagc atcgaccgcc tgctgaataa aaagtggtaa
     4441 gcacgcgcta tacttaacga taaaaaacga ggaggaagct cctcgttttt gctattggag
     4501 gagagaaaaa tgggtcaact gattgacggc gtctggcatg acacctggta cgataccaaa
     4561 tctaccggcg gtaaatttca acgttcagct tccgcatttc gtaactggct cactgccgat
     4621 ggcgcacctg gccccactgg cacaggcggt tttatcgcag agaaagatcg ttatcatctc
     4681 tatatttcac tcgcctgccc gtgggcgcac cgcacgctga tcatgcgcaa actcaaagga
     4741 ctggaaccgt ttatttccgt ttccgtagtg aacccgctga tgctggaaaa cggctggacc
     4801 tttgatgaca gttttccggg agcaaccgga gatacgctct atcaacatga atttctgtat
     4861 cagctttatc tccacgccga tccacactac agcggacgag ttactgttcc cgtgctgtgg
     4921 gacaaaaaga accacaccat cgtcagcaac gaatcagcag aaatcatccg catgttcaat
     4981 accgcgtttg atgcgctggg cgcaaaagcg ggtgattact acccaccagc cctgcaaacg
     5041 aaaattgacg aacttaacgg ctggatttat gacaccgtta acaacggcgt gtataaagct
     5101 ggttttgcca ccagccagca agcctacgac gaggcggtgg cgaaagtgtt tgaatcgctg
     5161 gcgcgactgg agcagatttt aggccagcat cgttacctga ccggcaacca gctaaccgaa
     5221 gccgatattc gcctgtggac cacgctggtc cgttttgatc cagtgtatgt gacccacttc
     5281 aagtgtgata agcaccgcat cagcgattac cttaatctgt atggcttcct gcgtgatatc
     5341 taccagatgc cgggaattgc cgaaacagtc aatttcgatc acatccgtaa tcattacttc
     5401 cgcagccata agaccatcaa tcctacgggg attatttcaa ttggtccatg gcaggatctc
     5461 gatgaaccgc atggacgaga tgttcgcttc ggttaaaaaa aggcacctta cgaggtgcct
     5521 ctgataatta gctaagtgca tgttgttcat gtcggatgcg gcgtgaacgc cttatcaaac
     5581 ctacaaaaca tttatattca atatgttgca atgaccgcgc aggccagata agcgtagcgc
     5641 atctggcgat tttgcgcttg tcatcagtct taacgcgcct tcacagcact ttattaattc
     5701 cttgaacgaa tatttactgc catttcattc acgtctattc ttaatttgct gctttaaaaa
     5761 caagtgattg agcaaaattg aggcaaaaat ggactggtat ctgaaagtac taaaaaatta
     5821 tgtcggtttc cggggtcgtg cgcggcgcaa agagtactgg atgtttattc tggtcaacat
     5881 catctttacg ttcgtgctgg ggctgctgga taaaatgtta ggctggcaac gcgctggtgg
     5941 cgaaggcatc ctgacgacaa tctacggtat tctggtgttt ttaccgtggt gggcagttca
     6001 gttccgccgc ctgcacgaca ccgaccgctc ggcgtggtgg gcactgttgt tcttaatccc
     6061 gtttatcggc tggcttatta tcatcgtctt taactgccag gcgggtacgc caggcgaaaa
     6121 ccgctttgga ccagatccga aactggaacc ttaaaacaaa tctgctcgcc ggatgtctga
     6181 ctcatccggc ttttatcatc atgttgatga atgaataatt tactaatgtt tatttaaaat
     6241 atttcaatgt cgttatataa attacctata aaaaacaacc atgtatttat cttaaatatc
     6301 aaaaccttta taacatccga aaaagaaaga tacgaatagc atttacacaa tacccggaat
     6361 agactttcgt cacttttaat taaagggatg tttttatgca gtggtattta gccgtattaa
     6421 aaaattatgt tggtttctct ggtcgcgcac gtcgtaaaga gtactggatg tttactctga
     6481 ttaacgccat cgtcggcgct attatcaatg tcattcaatt gattttaggt ttagagtttc
     6541 cattcctttc tctcatttac ctggcagcaa caatcattcc ggtcattgct ctgtgtgttc
     6601 gtcgtctgca cgatactgac cgttcaggtg cgtgggcgct tctctatctg gtaccgatca
     6661 ttggctggtt agttctgttt gtatttgcct gtttagaagg caattctggc agcaatcgtt
     6721 acggaaacga tcctaagttt ggttcaaatt aatcttagaa ttggggcgac atttcgcccc
     6781 tttttattaa taataacaaa tattattttc cgctaaaaag tttgggaatt tcccgcagac
     6841 accaggattt cgcttccccc atactgtcac gacgccaggc cataataata tcgatctcgc
     6901 tggtcgattc cgggctgacg acacgcaacc gcccttccgc aatatctttt tcgaccatcg
     6961 gatacggcat cgtcgccacg ccaagccccg ccagtaatgc ctgacgttta tcttcaatcg
     7021 tgctcaccgt taagcgcggc tgtttgtcca gcagctgtac ggtcaacacc gggcgctcac
     7081 gagcggtatc cgctaccgca attccacgat atttcacgcg cgtcacttca gataacggtt
     7141 ccggctcctg atgaatcggg tgatcaggcg cggcaacgta gacgttcatt aaggtataga
     7201 gcttgcgcga gttgatctcc gacgaggaac gaaaatgcat atccggcgcg ataacgatat
//
LOCUS       DAZGAN020000178         7222 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGAN020000178 DAZGAN020000000
VERSION     DAZGAN020000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.178"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1001..1201
                     /locus_tag="ACHKYO_RS23485"
                     /old_locus_tag="pgaptmp_004697"
                     /pseudo
     CDS             1001..1201
                     /locus_tag="ACHKYO_RS23485"
                     /old_locus_tag="pgaptmp_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425060.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(1248..2354)
                     /locus_tag="ACHKYO_RS23490"
                     /old_locus_tag="pgaptmp_004698"
     CDS             complement(1248..2354)
                     /locus_tag="ACHKYO_RS23490"
                     /old_locus_tag="pgaptmp_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGU4431395.1"
                     /translation="MNKTITALTVMMSSFAANASVLPETPVPFKSGTGAIDNNTVYIG
                     LGSAGSAWYKLDTQAKDKKWTTLAEFPGGARDQATSVFIDGNLYVFGGVGKNNEGLTQ
                     VFNDVHKYNPKNNTWVKLMSHAPMGMAGHVTFVHNGKAYITGGVNQNIFNGYFEDLNQ
                     AGKDSKTTDKINANYFNKKSEDYFFNKFLLSFDPATQQWRYAGESPWYGTAGAAAVSS
                     GDKTWLINGEVKPGLRTDSVFELDFTNTGVNWSKLPSVSSPDGVAGSFAGISHDALIF
                     AGGAGFEGSRKNYQNGKNYAHEGLKKSYSADIYLWYNGKWGKSGELPQGRAYGVSLPW
                     NDSLLIIGGETTGGKAVADSVLISVMGNKVTVQN"
     gene            complement(2374..3087)
                     /locus_tag="ACHKYO_RS23495"
                     /old_locus_tag="pgaptmp_004699"
     CDS             complement(2374..3087)
                     /locus_tag="ACHKYO_RS23495"
                     /old_locus_tag="pgaptmp_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HGU4431396.1"
                     /translation="MKRYQVVSGILLCASFSVISNAATLDIRGGYRSGSHAYETRLKV
                     SEGWQNGWWASLESNTWNTIHDNKKENLALNDVQAEINYAIKLDDKWSVRPGMLTHFS
                     SNGTRYGPYIKLSWDVTKDLNLGIRYRYDWKTYRQEDLSGKMDRDNAHRWDGYLTYKI
                     NKDFTFAWQTTVYSKQNNYRYGNHKKDATENAFVLQYHMTPDITPYIEYDYLDRQGVY
                     NGKDNLSENSYRIGVSIKL"
     gene            complement(3231..3335)
                     /locus_tag="ACHKYO_RS23500"
                     /old_locus_tag="pgaptmp_004700"
                     /pseudo
     CDS             complement(3231..3335)
                     /locus_tag="ACHKYO_RS23500"
                     /old_locus_tag="pgaptmp_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462246.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(3335..3720)
                     /locus_tag="ACHKYO_RS23505"
                     /old_locus_tag="pgaptmp_004701"
                     /pseudo
     CDS             complement(3335..3720)
                     /locus_tag="ACHKYO_RS23505"
                     /old_locus_tag="pgaptmp_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3744..5231)
                     /locus_tag="ACHKYO_RS23510"
                     /old_locus_tag="pgaptmp_004702"
     CDS             complement(3744..5231)
                     /locus_tag="ACHKYO_RS23510"
                     /old_locus_tag="pgaptmp_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4431397.1"
                     /translation="MNTLQNKTPWYKHLNRSQWRAFSAAWLGYLLDGFDFVLITLVLT
                     EIQGDFGLTTVEAASLISAAFISRWFGGLVLGAMGDRYGRRLAMVVSIILFSLGTLAC
                     GLAQGYITLFIARLVIGMGMAGEYGSSVTYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRVLFFIGILPIIFALWLRKNIPEAEDWKEKHEGKAPVRTMVDILY
                     RGEYRIANIAMTLVAAVALWFCFAGNLQNALIIAGLGVLCAVIFISFMVQSSGKRWPT
                     GVMLMIVVLFAFLYSWPIQALLPTYLKTELSYDPSTVAQVLFFSGFGAAAGCCVGGFL
                     GDWLGTRKAYVFSLLASQILIIPVFTIGASSVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGAVAPVLGAVVAQRLDLGTALCSLSFGLTFVVILLI
                     GLDMPSRMQRWLRPEALRTHDAIDGKPFSGAGVIKNESSLEMKSR"
     gene            complement(5312..6205)
                     /gene="nanA"
                     /locus_tag="ACHKYO_RS23515"
                     /old_locus_tag="pgaptmp_004703"
     CDS             complement(5312..6205)
                     /gene="nanA"
                     /locus_tag="ACHKYO_RS23515"
                     /old_locus_tag="pgaptmp_004703"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015962808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGU4431398.1"
                     /translation="MTADLKGIMSALLTPVDAEQKLDQDSLRRLTRFNIEQGIDGLYV
                     GGSTGEAFVQSVQERESVLEIVADEAKGKLKLIAHVGCVSTNESQSLARAASRLGYDA
                     VSAVTPFYYPFSFEEHCEHYRAIIDAADGLPMVVYNIPALSGVKLSLEQISTLVTIPG
                     VRALKQTSGDLYQMEQIRRSHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYK
                     RIAGALQNGDIKTAKYLQSECNKVIDLLIRFGVFRGLKTVLHYMGIIDAPLCRKPFSG
                     VNAEYMPELKSLAETLMKEKT"
     gene            6651..7222
                     /locus_tag="ACHKYO_RS23520"
                     /old_locus_tag="pgaptmp_004704"
                     /pseudo
     CDS             6651..7222
                     /locus_tag="ACHKYO_RS23520"
                     /old_locus_tag="pgaptmp_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382922.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
BASE COUNT         2072 a         1656 c         1437 g         2057 t
ORIGIN      
        1 ttggccctca acctcaattt caagcagagc cctttttgat gccgcgccag cggcatggaa
       61 ggcggcactc tgtggttacc tgtggcacag gtgtaaaacc gccgaagccc ggcgtaagcc
      121 ggtacgtatt gaaaggtttt gcctatccca ttcccatcct gctaagccat acccgctttc
      181 agtcatgatg aagtaagagt gcgcggtcgg agtggtcccg acgagggttt acccgaagtc
      241 ggggcgtatc tccgcgttag cgggccgtca gggccgctta cgagcgcgta ttcggaacat
      301 ccagccagaa gactgacagc gatgactatg cttagttata acattcataa ttaaaagcga
      361 tgttgttccc gcccttcgga cgggggcggc ctcgcttttc agttatgagg ggtgggcacg
      421 gtgctgcgtt tgtgtggtgt ttttattcct gatgtttttt gttgtggttt cagaccggtg
      481 aggttcgggg cccgcgcgtt agcgtgggag gtgcttttag attctgagcg gcgcggcctt
      541 ggccgctgca agcgaaggcc tctttgtatt tgttcacagt ggtttcagag ataaagactt
      601 tccctgcggc gaagcaacgc aggggattaa ataatgaggt taagactata ttgcagcttt
      661 gctgtttcaa gtttgcagct tcttaaagtt ctttgtttgc ggatttatat ggataactga
      721 aacgacgtgg ccacgttctg atgactgatg cgtagtttta tgggcatata ggggtcagtt
      781 tggatataga gaattattgt acggcaagcc tgttttttga acagacttgc ctgtcgcaat
      841 agaatgtact cccgtgctat acggcgtttg cagggaattt gaccttaatc ggataaccta
      901 aagtgcgacg acttaaaaag cgatagccgc tgacgcaaaa tcaatccgtt acgcttctcg
      961 attgattaaa taatgctctt aacgtacaaa attttccgat atccaaaccg ccaccgagcc
     1021 tagtggttta ggtagttttc ttgttgcgtt acaccaggta attgaggcgc gaagaaggga
     1081 tttctgaagc tgccaggaaa tctggccttg cccgccagag tatttacccg cattatcccc
     1141 gaatggaacc ccgaccatca caacacttgc acaactaaca tctggaacct tatttaaatg
     1201 acaccaaaac attgataaca gggctcccta accggagccc tgatgagtca gttttgtacc
     1261 gtaactttat tacccatcac tgagatcaaa acggaatccg caacagcttt acctccagtc
     1321 gtttcacctc caataatcaa taagctgtca ttccagggta atgacactcc gtaggctcgc
     1381 ccctgtggta gttcacctga tttaccccat tttccgttat accagagata aatatcagca
     1441 ctgtaggatt ttttaagacc ttcatgcgca taattcttac cattctggta atttttccgc
     1501 gaaccttcga agccagctcc tccggcaaat ataagcgcat catgacttat tcctgcaaag
     1561 ctacctgcaa cgccatcagg tgatgagacg gaaggtagtt tactccagtt tacccctgtg
     1621 ttcgtaaagt ccagttcaaa cacgctatcg gttcgtagcc cgggtttgac ttcgccatta
     1681 ataagccagg ttttatcacc ggaactcact gctgctgcac cagccgtacc ataccaaggg
     1741 gattcaccag cgtaacgcca ttgctgtgtc gcagggtcaa aagacaacag aaacttatta
     1801 aagaaataat cttctgactt tttattgaaa taattagcat taatcttatc tgttgttttt
     1861 gaatcttttc cggcttggtt aagatcctca aagtatccat taaatatatt ctggttaacg
     1921 ccacctgtaa tataagcttt accgttgtgg acaaaggtca catgccccgc catccccatt
     1981 ggcgcatgcg acatcaattt aacccaggta ttgttttttg ggttatattt atggacgtca
     2041 ttgaatacct gtgttaatcc ctcattgttt ttaccaacgc cgccaaatac atacaggtta
     2101 ccatcaataa aaacagaggt tgcctgatct cgcgcccccc ccgggaactc agccaaggtt
     2161 gtccattttt tatctttagc ctgagtatcc agtttgtacc atgcggaacc agcgctacct
     2221 aaaccaatgt aaactgtgtt attatcaatt gcaccagtac cacttttaaa cggtactggt
     2281 gtttccggta gtacggacgc gtttgccgca aatgaactca tcatgacagt aagcgctgtt
     2341 attgttttat tcattgtaac agtctcccgt tcgttacagt tttattgaca caccaatacg
     2401 ataactattt tcagataaat tatctttgcc gttgtaaaca ccctgacggt caaggtaatc
     2461 atattctata tatggcgtaa tatcgggtgt catatggtat tgcagaacaa atgcattttc
     2521 agtcgcatct ttcttatggt taccatagcg ataattgttt tgtttgctat aaacagttgt
     2581 ttgccatgca aaggtaaagt ctttatttat tttatacgtt aaatatccat cccaacgatg
     2641 cgcattgtct cgatccattt tgccagaaag gtcttcctgt ctgtaggttt tccagtcata
     2701 ccggtaacgg atcccaaggt tcaaatcttt tgtaacatcc cacgaaagtt taatatatgg
     2761 tccatagcgt gtgccattac tgctgaagtg tgtcagcatc cctggacgaa cagaccattt
     2821 atcatcaagt ttaatagcat aattaatttc tgcttggaca tcattcagtg caagattttc
     2881 ttttttatta tcatggattg tattccacgt attactttca agacttgccc accatccatt
     2941 ctgccaccct tcgctcactt ttaatcgggt ttcataggca tgactgccac ttctatatcc
     3001 tccccggata tccagtgttg cagcattaga tataacagaa aatgaggcgc acaataatat
     3061 accagaaact acttgatatc ttttcatata tatacctttt gtcgaacaca tgattagtta
     3121 tgtggagcta taaaatcaac ataattcttt ataaaaaatg tcctttttat tatttttctg
     3181 ccagtaatgc agcaccaatt attcctgcat tgttacggta agtggcagga acttcccgga
     3241 gtgggaattt cacgccggga tgaaatgtta agggtatcat caaccagtgc agctgccagt
     3301 ttcgtaccac cgatatctat tgccagagta cgcattatct gagagcctcc tgaatggctt
     3361 tacaatacca gtgacagatg tgttcctgat gagtaatggc agcccccact gttacagccc
     3421 aggttccatt gctgcagcca aagccggggg cttatcagta ataacgcggc aacctttctt
     3481 gcttaatctc ctgactaaat ccagataggg agtatgatca cctgtgtaac cagacaggct
     3541 ggttacaata atttctgtgc cctgtctgta acatttcgaa ccatcctgtt ctgaagaaca
     3601 gtcagccatt gccgtgatca tctctggatc atccattgga gcagcctgaa caaagtggca
     3661 ggaaacgatc acccatccct gtttttttac tttttcgtcc ataagctgtg gtaatgtcat
     3721 aactactccg tttgttacag gggtcagcgg gatttcattt ccagagaaga ctcattttta
     3781 atcacaccgg caccactgaa tggcttcccg tcaatagcat catgtgtacg cagggcttcg
     3841 ggtcttaacc agcgctgcat ccgggaaggc atatccagac ctatcagcag gatgactaca
     3901 aaggtcagac cgaacgagag cgaacacagt gctgttccga gatcaaggcg ttgagcaaca
     3961 acggccccca gtacaggagc aactgctccc cccagagcgc ctacgttata agtgaatcca
     4021 aggccggcag cacgctgatc agtatcaaaa taaccgccaa taagttttgg aagaataccg
     4081 gcaatacctt gccccaacat ttgctgaaaa aagagtaata aacccagtac ccatacactg
     4141 gatgcaccaa tggtaaagac aggaatgatc agaatctggg aagccagcag actaaataca
     4201 taggctttac gggttccaag ccagtctccc agaaaacctc cgacgcaaca tccagctgca
     4261 gcaccaaatc cactgaaaaa aagaacctga gcgactgtgg aagggtcata tgacaactct
     4321 gttttcagat atgtgggtaa cagagcctga attggccagg aataaagaaa cgcaaacagg
     4381 acaacgatca tgagcatgac gcctgttggc cagcgtttac cactactctg aaccataaat
     4441 gaaataaaga taactgcaca caaaacgccc agaccagcaa ttatcagagc attttgtagg
     4501 ttcccggcaa aacagaacca aagagcaaca gcagcaacca gagtcatggc aatatttgca
     4561 atccgatatt caccacggta caaaatatcc accattgtac ggacaggtgc tttcccttca
     4621 tgtttttctt tccagtcctc tgcttccgga atatttttac gcagccagag agcaaaaata
     4681 ataggaagaa tccctataaa gaacagcact cgccatcccc atactggtac aaccagacta
     4741 taaacctgag ctgcaacaac tgcacctact gaaaagccag aaatcagaaa gccacttgct
     4801 ttattgcgga gatgctttgg ccagctttcg atgacgtatg ttacactact gccatattca
     4861 cctgccatcc ccataccaat aaccagacga gcaataaaga gggttatata tccctgagca
     4921 aggccacacg ccagtgttcc aagggaaaaa agaataatac tgacaaccat cgccagacgg
     4981 cgtccatatc tgtctcccat tgctcccaga acaagtccac caaaccaacg ggaaataaat
     5041 gcagcggata taagactggc tgcctcgacc gttgtcaggc caaagtctcc ctgaatctcg
     5101 gtaagaacaa gagttatcaa cacaaaatcg aaaccatcga gtaaataacc tagccaggcg
     5161 gcagaaaatg cacgccactg gctacgattt aaatgcttat accatggcgt tttattttgt
     5221 agggtattca taaataacaa cctcacatac agatgttcct gtttattcag ttatcttaaa
     5281 ctttatataa caaagaatat gtaaccgtac tttatgtttt ttctttcatt aaagtttctg
     5341 ccagagattt aagttcaggc atatactctg cattaacacc agagaatggt tttctgcata
     5401 atggggcatc aatgattccc atatagtgaa gtactgtttt taatccccgg aacacaccaa
     5461 acctgattaa aagatcaata actttattac attcactctg aagatatttt gccgttttta
     5521 tatcaccatt ctgcagtgca ccagctattc ttttatatct ccatcccata atgttatatg
     5581 tactacctat cccgccatct gcacctgcca gcaatccgga tgcaaatatc tcatcataac
     5641 cattgtaaag aaccaagtct ggatgagacc gacgaatctg ctccatctga tataaatctc
     5701 cggaggtttg tttaagtgct ctaacaccgg gtatagtcac cagtgtgcta atttgctcga
     5761 gagataactt tactccactc agtgcaggga tgttatatac caccatcggt aatccatctg
     5821 cggcatcaat aatggcacga taatgttcac aatgctcttc aaaagaaaag ggataataaa
     5881 aaggtgtcac tgctgaaaca gcatcatagc ccagtctgct tgctgcccgg gcgagactct
     5941 gactttcgtt tgtactcaca caaccaacgt gagcaatcaa cttcagtttt ccttttgctt
     6001 catcagcaac gatctccagc actgactcac gttcctgtac actctgtaca aaggcttctc
     6061 ccgtagagcc accaacatac aatccatcaa tcccctgctc tatattaaat cttgtcaacc
     6121 tgcgcagact gtcttgatcc agcttttgtt ctgcatcaac aggtgtcagc agagcagaca
     6181 taatgccttt taaatcggca gtcattgttt acctcttcaa caattcagta tacctttata
     6241 cctattacac cagtggttct gtacaaatcc atatggaaaa aatggttttg tgataatggt
     6301 tacaaaattt gtctgccagt tgccagaaac gtacaaatat atcaatgcaa atattgaaat
     6361 ctcatctgga taaaacaatt taaatcgacc ctcaggccaa ttctaaacat tagtatatct
     6421 aatgatttgt tattttatac ttgtataacc gttgtataag gctggggttc cggaatgata
     6481 ttaataatga tgactgttat ttgtagtttg aatttttagc cacgatgaac gagaatatta
     6541 atgcataaac tatgctcaat cagatgaatg attcagtctc tgatgtatct gtaaaattat
     6601 attttgtttc attcatgtaa cttaagtgtt gatttttaaa ctggtaaatt atgtttgata
     6661 aacgaccgtt aaaaagaaaa aaactctctg agttagttga ggaagaactg gagtttatga
     6721 tcagaaaagg aatttttcag gagggtgaac atataccgtc agagcggaca ctgatggaaa
     6781 tgtttaaagt tggcagacca tctgttcgtg aagccttggc tactttacga cgtaaaggac
     6841 ttattgaagt taataatggt gaaagaacga aggtttgtcg acccactcct tttactctta
     6901 taaatgagtt atctgggatt gccagagatt atctggccag cgacgaggga atgcaacatt
     6961 ttgaacaatt acgacttttt ttcgagttaa tgttagtcag atatgcttcg gtttatgcaa
     7021 acaaggagca aaaaaaataa acttaatcag gctgcaaaca tttgttggaa atatgttgat
     7081 gataatatca actttattcg tgcagatatt aacttccatc gggttttatc tgaaatccct
     7141 gaaaaccctg tatttgttgc tattcataca gcactaacag actggttgat aaatacacat
     7201 tctgaagagc acagtaatat aa
//
LOCUS       DAZGAN020000179         7147 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGAN020000179 DAZGAN020000000
VERSION     DAZGAN020000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.179"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            99..1433
                     /gene="adeQ"
                     /locus_tag="ACHKYO_RS23525"
                     /old_locus_tag="pgaptmp_004705"
                     /gene_synonym="yicO"
     CDS             99..1433
                     /gene="adeQ"
                     /locus_tag="ACHKYO_RS23525"
                     /old_locus_tag="pgaptmp_004705"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HGU4431399.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1486..1938
                     /gene="yicN"
                     /locus_tag="ACHKYO_RS23530"
                     /old_locus_tag="pgaptmp_004706"
     CDS             1486..1938
                     /gene="yicN"
                     /locus_tag="ACHKYO_RS23530"
                     /old_locus_tag="pgaptmp_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HGU4431400.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2149..3339
                     /gene="nepI"
                     /locus_tag="ACHKYO_RS23535"
                     /old_locus_tag="pgaptmp_004707"
     CDS             2149..3339
                     /gene="nepI"
                     /locus_tag="ACHKYO_RS23535"
                     /old_locus_tag="pgaptmp_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HGU4431401.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3380..3673)
                     /gene="yicS"
                     /locus_tag="ACHKYO_RS23540"
                     /old_locus_tag="pgaptmp_004708"
     CDS             complement(3380..3673)
                     /gene="yicS"
                     /locus_tag="ACHKYO_RS23540"
                     /old_locus_tag="pgaptmp_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HGU4431402.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3895..4713
                     /gene="nlpA"
                     /locus_tag="ACHKYO_RS23545"
                     /old_locus_tag="pgaptmp_004709"
     CDS             3895..4713
                     /gene="nlpA"
                     /locus_tag="ACHKYO_RS23545"
                     /old_locus_tag="pgaptmp_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HGU4431403.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4717..5640)
                     /gene="yicL"
                     /locus_tag="ACHKYO_RS23550"
                     /old_locus_tag="pgaptmp_004710"
     CDS             complement(4717..5640)
                     /gene="yicL"
                     /locus_tag="ACHKYO_RS23550"
                     /old_locus_tag="pgaptmp_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HGU4431404.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5751..6935)
                     /gene="setC"
                     /locus_tag="ACHKYO_RS23555"
                     /old_locus_tag="pgaptmp_004711"
     CDS             complement(5751..6935)
                     /gene="setC"
                     /locus_tag="ACHKYO_RS23555"
                     /old_locus_tag="pgaptmp_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HGU4431405.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1801 a         1602 c         1799 g         1945 t
ORIGIN      
        1 cattaatgtg caattgaaat gtgataatta tcactgaacc gttgttgtca tttgttttac
       61 aaaagcaagg gattaaatct cctccaatgg acaaaaaaat gaataatgac aataccgatt
      121 acgtgagtaa tgaatcaggg acgctttcgc gattatttaa actacctcag catgggacca
      181 ccgtccgcac agaattgatt gcggggatga ccactttttt aaccatggtg tacatcgttt
      241 ttgtgaaccc gcaaatcctc ggcgcggcac aaatggaccc gaaagtggtg tttgttacca
      301 cctgtttgat tgccggtatc ggcagtattg cgatggggat atttgctaac ttacccgtgg
      361 cgctggctcc ggcaatgggg ctgaacgcct tctttgcctt cgtggtcgtg ggggcgatgg
      421 gcatctcctg gcagaccggg atgggcgcaa tattctgggg cgcagttgga ctatttttgc
      481 tcacgctgtt tcgtatccgg tactggatga tctccaacat tcccttaagt ttacgtattg
      541 gtatcaccag cggaattgga ttatttatcg ccttaatggg attaaaaaat actggcgtta
      601 ttgtcgccaa taaagacacg ctggtgatga ttggcgattt aagttctcac ggcgtgttgt
      661 taggtatttt agggtttttt attataaccg tgttgtcatc acgtcatttt catgccgcgg
      721 tgctggtttc tattgtggtg acgtcttgct gtggattatt tttcggtgat gttcatttta
      781 gcggcgtcta ttccattccg cctgatatta gcggcgtcat tggtgaagta gatttgagcg
      841 gcgcgttaac acttgaactc gccggtatca ttttctcctt tatgctgatc aacctatttg
      901 attcatcagg aacattaatt ggtgtaactg ataaagcggg cttaatagat agtaacggta
      961 aattccccaa tatgaataag gcgctgtatg ttgatagcgt cagttcggtg gcgggggcgt
     1021 ttatcggcac ctcgtctgtt actgcctata ttgaaagtac ttctggtgtg gcagtcggtg
     1081 gtcgcacggg gctgactgcg gttgtggttg gcgttatgtt cctgttagtt atgttcttct
     1141 caccgctggt ggcgatggtt cctccttacg caaccgccgg agcgttaatc tttgttggcg
     1201 tgctgatgac ttcgagcctg gcgcgcgtta actgggatga ttttaccgaa tcggtgcctg
     1261 cgtttattac cacggtgatg atgcccttta ctttctcgat caccgaaggg attgcactcg
     1321 gctttatgtc gtactgcatc atgaaagtat gcaccgggcg ctggcgcgat ctgaacctgt
     1381 gtgtggtggt ggtcgcagct ctgtttgcac tgaagattat tctggtggat tagtaactgg
     1441 cgagggtttc cagatatcat gagttctgat tacgcaggag aactcatgat ctggataatg
     1501 ctcgccacgc tggcggtagt gtttgtggtt ggttttcggg tgctgacatc cggggccaga
     1561 aaagcgattc gccgtctcag cgatcggctg aacatcgatg tcgtacccgt ggagtcgatg
     1621 gtcgatcaaa tgggaaagtc agctggtgac gaatttttac gttatttgca tcgtccggat
     1681 gagtcgcacc tgcaaaacgc cgcgcaggtg ttgctcatct ggcaaattgt cattgtcgat
     1741 ggtagcgaac agaacctgct gcaatggcat cggattttac aaaaagctcg cctcgccgcg
     1801 ccgattaccg acgctcaggt caggctggcg ctaggttttc tgcgcgaaac cgaacctgaa
     1861 atgcaggata ttaatgcttt tcagatgcgc tataacgcgt tctttcagcc tgccgagggc
     1921 gttcactggt tgcattgaaa ccgtttcgtc ttatcagcct acagaccgcg cctgacctgt
     1981 cgggtggata agacgttcac gccgcatccg gtaatctttg ccctgttccg tttcgatctt
     2041 aactccagct caagcaacac cgtaaaagtt gcaaaaacgt taaatgcgtc acacatttca
     2101 atgttaaagt ggtcggcttt tcccctgaaa catgccacgg gtaacaccat gagtgaattt
     2161 attgccgaaa accgcggcgc ggatgccatc acccgaccga actggtcagc cgttttctcg
     2221 gtggcgtttt gtgtcgcctg tctgattatc gttgagtttt tgcccgtcag tttgttgacg
     2281 ccaatggccc aggatttagg catttcggaa ggggttgccg ggcaatcggt gaccgtgacc
     2341 gcctttgtgg caatgtttgc cagtttgttt attacccaga caattcaggc tactgaccgc
     2401 cgctacgttg ttattttgtt tgccgttttg ctgacgctct cctgcttgct ggtttccttt
     2461 gctaactcat tcagtttgct tttaatcggt cgtgcctgtc tggggctggc gctgggcggg
     2521 ttctgggcga tgtcggcgtc gctgaccatg cgtctggtgc cgccgcgtac ggtgccgaag
     2581 gcgctgtcgg tgatcttcgg cgcggtttct attgcgctgg tgattgccgc gccgttgggc
     2641 agttttttag gcgagcttat cggttggcgc aatgtcttta atgcggcggc ggtgatgggc
     2701 gtgctgtgta ttttctggat tatcaaatca ttgccttcac tgccaggcga accctcgcat
     2761 cagaaacaaa atactttccg cttattacaa cgtccgggtg tgatggcagg gatgatcgcc
     2821 atcttcatgt ctttcgccgg gcagtttgct ttcttcacgt atattcgccc ggtgtatatg
     2881 aacctggcgg gattcggcgt ggatggctta acgctggtgc tgttgagttt tggtatcgcc
     2941 agctttattg gtacgtcgct ttcgtcgttc attcttaaac gttcggtaaa actggcctta
     3001 gcaggcgcgc cgttaatact ggctgtgagt gcgttggtac tgacgttgtg gggaagcgat
     3061 aaaatcgttg ctaccggcgt ggcgattatc tgggggctaa cttttgcatt ggttcccgtc
     3121 ggctggtcaa cgtggatcac ccgctcgctg gccgatcagg cagaaaaagc cgggtctatt
     3181 caggtggcgg ttattcagct tgctaatacc tgtggcgcgg caatcggcgg ttatgcgctg
     3241 gataatattg gtctgacttc gccgttgatg ttgtccggca cattgatgtt gctgactgca
     3301 ttgttggtta ctgcaaaggt gaaaatgaag aaatcctgat atcaggtaaa gaagcgttgg
     3361 tcagtagttg ttgctgttct tacatatctg ggcattctac cttacccaac gcttcccaat
     3421 aattgctctg attattacgt tccatcgcca cgatggcctc gcgtatatgt tgtttattgt
     3481 tttcatctga caacattaac ttttcccacg cttcatctga taatgacgcc tgtggcgtgc
     3541 aaattttacg taaatcttgt aataccgtgg ttttctcttt tgcggactgc agtgagctaa
     3601 aaggcgactc ggcgtatacg atgcctggca tagcgaaaaa agtgaaaata agcagtagtg
     3661 tcgttggttt catggattca accttttaca aatgtgtatc gaatgaagca taagtgtagt
     3721 ttgctttcat cgctctcatc atgctgtacg aaacgcgctt gcgtggtcag taagaagtgc
     3781 cagactttat attccacttt tattcctttt tattcttatc gatagcgttt cgttttttaa
     3841 accgcagcga ccttaccgct atagtcaggt aatcattaat aaaaggataa aaaaatgaaa
     3901 ctgacaacac atcatctacg gacaggggcc gcattattgc tggccggaat tctgctggca
     3961 ggttgcgacc agagtagcag cgatgcaaaa cacattaaag ttggcgttat aaatggcgca
     4021 gaacaagatg tcgcggaagt cgctaaaaag gtggcaaaag agaaatatgg cctcgatgtt
     4081 gaactggtag gatttagcgg ttcgttgcta ccgaacgatg cgacaaatca tggcgaactt
     4141 gatgcgaatg tcttccagca tcgacccttt cttgaacaag ataatcaggc gcatggttat
     4201 aaactggtag cggtgggaaa tacgtttgtc ttccctatgg cgggttattc caaaaaaatc
     4261 aaaaccgtgg cgcaaataaa agaaggcgcg acagtggcga tccccaacga cccgaccaac
     4321 cttggtcgcg cacttttact gttgcaaaaa gagaagctaa ttactctgaa agagggaaaa
     4381 gggttattac ctaccgcgct ggatataacc gataatcctc gtcatttgca gattatggaa
     4441 ctcgaggggg cgcagctccc gcgcgtactg gatgatccca aagttgatgt agcgattatc
     4501 agcaccactt acattcagca gacagggctt tctccggtgc acgacagcgt atttattgaa
     4561 gataagaatt cgccgtatgt gaatattttg gtggcacggg aagataataa gaatgcagaa
     4621 aacgtgaagg aatttctgca atcttatcaa tcacccgaag tcgctaaagc ggcagaaacc
     4681 atttttaacg gtggcgcggt gcctggctgg taattctcac tcatgccgcg ccggacgatt
     4741 tatttttctg gcgcggcgac gggaatccat tgaaatcaaa atcaccgatg acagaatcag
     4801 cagcgttccc agccagtccg gtaatgtgaa cgtgatcccc agcaacagca aagagagtag
     4861 cgcgctactt aatggttctg cacagctcaa aatgctggct tttggaccgc caattaattg
     4921 tgctcctttc aggtacagac taaatgtcag ggacgtacca atgaccacca aataaaaaaa
     4981 cgccagaatc aaactgccgt taacgacaaa gtttgttcct tgtctggcat aaaaaggcaa
     5041 cagaatcaga ccgccaatca gcatactcca gccgacgact ggtaacgtgc catagcgggc
     5101 aattagcgtt gagggatagg tggtatagaa tgcagcagca aatgccgagg caatgcccca
     5161 gaacaacgcg gcaggagaga tcgataatga cgtcggatta ccgtgtgtca ccaataaaaa
     5221 agtcccgacc agcgatgtca aaatagcgca gaaaaccaga atgcccgggc gcgatttacg
     5281 caccagtgag aaccaggcga cgataatcgt cggtgagagg aattgcagca ccgttgccgt
     5341 ggctgcgttc gatttttcga tggttagcaa aaaagtgagc tgtacagtta gcgcgccaac
     5401 cacggaaaaa atcagcaggc taatggcatc tttatgattg ttaataatag aaaagatttt
     5461 atcgccatga acaaatgaca gcgtcagtag aatcaaaccg gcgaatatca aacgcgtcat
     5521 agtcaaaaac tgcgacgaca tctggctttg ctccatgatg tattgcgcgc agacccctga
     5581 acttccccac aacacggcgg caatcagaac gttcagcatc ccctttctgg tggaacccat
     5641 ttttccctct gcatactgtt ttgtcgttta tgtatgcagg catgatagca aaaatggcga
     5701 ggatggctct gtttgcgcca cgccgatttt tcttagccca gactggtgag ctaaatatct
     5761 ttaataaaca gcaggcaaat catcgcaata cccaacatcc ctatcgccag ccagaacaac
     5821 gcatggtagc tccagatttc aaccataatt ccgtcaacag agccggcgat aatccagccg
     5881 acgcgtgaag tatttgcata taacgttgtc gcagagccta ttttttcagg catcaagtcc
     5941 tgaaaataaa gcatgccgat accacagaga ataccaagga agatggcatt aagaatttgc
     6001 aattccagct caaccgccgg agtcgtcgcc atgagtacgc tggcgtaaaa acacattcca
     6061 ctcacgatag caatgagcat taatagtcgc ttgccaatac gtttcatgta atagcctgcg
     6121 attaacatca tcggaatttc cagaccggca gcgataccaa tcatttctcc agccagttta
     6181 tcggttagat gcagttcatc aataataaat agcggcatat ttatcatgta gagattattc
     6241 gccgcccaca tcattgaaca gaccacaaaa agtagccgcg tatcccgctt cctgtgggtg
     6301 gagggtaaaa tttctacggg ttgggtaacg acaggaatat ttctttgtat tgatggcaaa
     6361 aacaaccaga ctatcagtcc gcaaacaaca aatgcgatgg ctgcggtgag atacatcact
     6421 ttaaaactaa atcccattgc cagttcataa gcgagcggtg gcccgataac ccaggcaagc
     6481 gagatctgag cacgtaaaaa tgtactgaac atgaccgtct cacggcctgt tctgtcggcg
     6541 tgttcacggg cgagggcgaa catttgcggg tttgcggtgg aagcaaaact actcagaagt
     6601 acgcccgttg agaggagaat gaagtagttg cgattccacg caaaaagcgt gcaggccagc
     6661 actccaaata agcaacatag cagaatcagt aatttacggt cgccttgttt atcggagtgc
     6721 cttgccagaa attgactgac cagaattccc ataatagcgc taccggtgaa gaaaaaacct
     6781 accattatag gacgggcttt cagttcatct gcgaggaata tacttagggt aggagtctga
     6841 agagcgcccg caatacccgt cagaaaggcg acaagtaaaa atgccgcggc agtgagatca
     6901 agtatttttg atggagtggt agccgttttt tgcattcttc tgaatcaccg ggcgaaaaaa
     6961 tataactgag ttacaaataa gtaccagtcg aatgagtatt gtattaagaa tttttgagta
     7021 tcgctgatac aaccagaaag caatgctagc gcaatattat agggccagca attgagatta
     7081 ttagtaaaag catgttaaga attaattacc gaatttatat attaattata gtaaggcaat
     7141 gagctat
//
LOCUS       DAZGAN020000180         7135 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGAN020000180 DAZGAN020000000
VERSION     DAZGAN020000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.180"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..935)
                     /locus_tag="ACHKYO_RS23560"
                     /old_locus_tag="pgaptmp_004712"
     CDS             complement(<1..935)
                     /locus_tag="ACHKYO_RS23560"
                     /old_locus_tag="pgaptmp_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGU4431406.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVG"
     gene            complement(935..2641)
                     /locus_tag="ACHKYO_RS23565"
                     /old_locus_tag="pgaptmp_004713"
     CDS             complement(935..2641)
                     /locus_tag="ACHKYO_RS23565"
                     /old_locus_tag="pgaptmp_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4431407.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            complement(2622..3473)
                     /locus_tag="ACHKYO_RS23570"
                     /old_locus_tag="pgaptmp_004714"
     CDS             complement(2622..3473)
                     /locus_tag="ACHKYO_RS23570"
                     /old_locus_tag="pgaptmp_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4431408.1"
                     /translation="MAKLDWKKLEQAFRREHARTGIKLQDWCRKNNISYGTARRYIKL
                     RKNPPINTEESAQKNAQKSAQIDDQKSAQKNVQGSDDESSNDKAYSDDGSGEKCAKNC
                     ANSAETKRNSRSGNPHPVARFSDRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVF
                     TRARALSVTKALKGMFADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLT
                     LDVLAETAPKLRADRERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGR
                     LDDIPEE"
     gene            complement(3726..4316)
                     /locus_tag="ACHKYO_RS23575"
                     /old_locus_tag="pgaptmp_004715"
     CDS             complement(3726..4316)
                     /locus_tag="ACHKYO_RS23575"
                     /old_locus_tag="pgaptmp_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4431409.1"
                     /translation="MNYPTIVNGIDFRDLVFVTGNEPVTDTRKVAVAFGKEHKDVLRK
                     TRAVVQQCSKEFAERNFALCYENNELQNGKPQPIYRMTKDGWTMLVFGFTGKAAMTFK
                     EAYIQAFNWMAELIQQGLANLEAERNAVMLEYMKEKDVASMSGRLLNRWGRVKKPQLL
                     ARLDRLEQQGQIGYPDLIKAFQPDIAMRRIVAVFPH"
     gene            complement(4555..4740)
                     /locus_tag="ACHKYO_RS23580"
                     /old_locus_tag="pgaptmp_004716"
     CDS             complement(4555..4740)
                     /locus_tag="ACHKYO_RS23580"
                     /old_locus_tag="pgaptmp_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4431410.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPG
                     PDWQTPLNGIISSSENG"
     gene            complement(4962..5070)
                     /locus_tag="ACHKYO_RS23585"
                     /old_locus_tag="pgaptmp_004717"
                     /pseudo
     CDS             complement(4962..5070)
                     /locus_tag="ACHKYO_RS23585"
                     /old_locus_tag="pgaptmp_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5292..5825)
                     /locus_tag="ACHKYO_RS23590"
                     /old_locus_tag="pgaptmp_004718"
     CDS             complement(5292..5825)
                     /locus_tag="ACHKYO_RS23590"
                     /old_locus_tag="pgaptmp_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4431411.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDHVNAIERDKALAWVEKNIRVPLTDPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            complement(5830..6045)
                     /locus_tag="ACHKYO_RS23595"
                     /old_locus_tag="pgaptmp_004719"
     CDS             complement(5830..6045)
                     /locus_tag="ACHKYO_RS23595"
                     /old_locus_tag="pgaptmp_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4431412.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(6122..6394)
                     /locus_tag="ACHKYO_RS23600"
                     /old_locus_tag="pgaptmp_004720"
     CDS             complement(6122..6394)
                     /locus_tag="ACHKYO_RS23600"
                     /old_locus_tag="pgaptmp_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4431413.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            complement(6420..6602)
                     /locus_tag="ACHKYO_RS23605"
                     /old_locus_tag="pgaptmp_004721"
     CDS             complement(6420..6602)
                     /locus_tag="ACHKYO_RS23605"
                     /old_locus_tag="pgaptmp_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4431414.1"
                     /translation="MSFIHQIMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            complement(6748..>7135)
                     /locus_tag="ACHKYO_RS23610"
                     /old_locus_tag="pgaptmp_004722"
     CDS             complement(6748..>7135)
                     /locus_tag="ACHKYO_RS23610"
                     /old_locus_tag="pgaptmp_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4431415.1"
                     /translation="LYWPVSQLPENVVLEGSAGNDMLAAFFIQSDSANLNLMQHKQQN
                     AKLGTFGAFNHNWHNVVFRFAGNNSISVTPVINGQTQAAFNLAKCPAAGLDNDKLLLT
                     DITSNQDTYPVLIEHVTVAINNSNAA"
BASE COUNT         1609 a         1979 c         1632 g         1915 t
ORIGIN      
        1 cccacaaacc aggcttcacg aatacgactt acccgcccga ctttcacctg aacccgcccg
       61 gatgccacag ccaccgcctg catcagatta tttttatcaa aagccaccac ccgtccatta
      121 ctgagttcaa tcaccggaag acgctcgaat gtacggtaat aaaccacctg aagcagcaca
      181 cgacggcgtt cacgctgaag ccattcattc tccctgcgat cccatgactg atactcttcc
      241 catgcactca tcaacggact gggctggcct tcagtaatcg tggtatcgac aaaaccacgc
      301 cagtcatcaa tagcataatc gataacctga gccattcccg ggaacgtggc ttttgcctca
      361 tcggtatcca tccagcggcg acgcatcagc caccggcagt cactcaggtc ggattcccgg
      421 ctgagccagt cccagaaaac ctcatttctg ctgactgtgg ataccctgaa ttcaggtccg
      481 aacggatcgc tgttgcgcct gacctccacc caactgagcc ccgccttgat ttgttccgca
      541 taggcatcgg agcgggcctt attcatgttg ccaagacggc acgcatcagc aaactccgca
      601 ttaatggcct ctgccagttt ctctgtttca tcgttcggat cgtctgacat cactatcagg
      661 tccgttcttg tttttgcctc cattcccagt acaccatcta ccgtgggggc aatgaggtta
      721 tggatggtca tgggctgacc gcgatctttc agtacctgga taacttccgg tgccagctgg
      781 tcgccatcat aatacgcaca ggccttgttt gcggcatcac gccagagagg ctgactgtca
      841 atatcagaac agagagacag taactgacgc tgagaaaaac gcggcgtgga tccatgatcg
      901 tttttcatcg ctgtggtgtt aatttcattt ttcatcagtg tgccatccag tgtgtggttc
      961 tgcgtttatc cgttttctgt tttaccctca ccggcattct ggcgcgcatc tcctgggcaa
     1021 tcatgtagct catgagctga tcatcaaagc agccttcctg tgcattcatg gagcctttcg
     1081 cgtcataaac gtaggtgttc atttccgata atgtgcctga ccagcggatc cctgatattc
     1141 cattattcag gagcgttttc attccttcgg tcagaacagg tttgctctga cgggttgtca
     1201 gccagccaag gcggggcgta tcgtcgtcat atgcctggtc aagatgctgt tcgttgtaga
     1261 tataacgtgt cggatagagt tcccggagtt tcaggataac tgcatgtccg tgattattac
     1321 gctccggccc cacaaacgcg ttgttataca tacgacagac ctgcgaaatg agatgagcaa
     1381 aaagttcagc atcgagatgc ccgaaccagt gagccacctg ctcgccatta ctgcgtttga
     1441 caacatccag cgatgagcgg tctccgtgct ccagcccttc ggcagtatct gccccacaaa
     1501 catactcttc atccggatcc ggcagttccc ataccagcag ataattcatc agcgtccgct
     1561 gcaactcgtt tttatttcct tcacgcagag actgagcttt agtcttcgct cctgtaacag
     1621 gttcaatgtc ataaacaatc atcggtggcg aacagaatga ttctgcctgc aatgtacttt
     1681 cggcactgaa cacacgtcgt ccggacgtca gaaacgcctc ctgtggcgtt gagggaaact
     1741 cctgcttcat ttcctcacgc tgttcagttt ccttactgat gtaccactgc ttctgctcat
     1801 cggtaagcgt gatgttcatt gccttctcaa ccgcagaaaa atacgtcatt ttttcccgtg
     1861 acagcttcag cccgctttca ggcactctgg cgctgtattt aggatcctgc caccatgcgt
     1921 aaaaatggaa tttataatcc tgtgccgtca gcaataagcc tgatgcagtg ctctcctgtg
     1981 cacggttact catctcgtaa aaatcaccac ccacgccttc agccgtggat tcatcaaaaa
     2041 taatgcattc atcagagacg gcattaagcg taccggttcg cagctctttc gccttagccg
     2101 gatatttcgc gcaaattttg ccgtgctctg agatatgcag gcgctgcacc gtacctgagc
     2161 gaaatgaggt tgccacctga atactcgagc cgtgaccaaa caggatatag ccaccgctgg
     2221 caccgctacg acgttcaacg atggtgaatg aggctctcag ccagtcaggg agatgatcaa
     2281 acggtacagc aatttttgtg cggaaaattt cactggcagc ctgtttatcc tgagcgacga
     2341 tcccgcattt gagatgcgga atgaataatg cctggtcgag aagataaata tcaatggctg
     2401 tggaaaatcc cagctggcgc gctttcagga taatgttttt attatgcatg ttacgaaaca
     2461 actgacgttg cgccggtcgc attctgaagg tgaccagttc acctttttcg ttctgtattt
     2521 tgtagagatg attgaggcgc caccagggat tgctcagttt tgtcataatg aacagacgtt
     2581 gttcggtctc agtcatttct gcaggttcat cacatcgcgt tttattcttc cggaatgtca
     2641 tccagtctcc ccgaattact catttcatgc agcgatgaca cgatgtcact gactggcgta
     2701 ataacacccc ggcgctggct ggtcagaata tcggtttccg ctctgagttt atctctggcg
     2761 gcgttgattc tttcccggtc agcacgaagt tttggtgctg tctcagccag gacatccagc
     2821 gtcagcaatg agcgttcaat tgactcgata cgggcaatat tccggtcaag ggcctgttca
     2881 gctttgagta ttttgtcgta aagagcaacg cgggtttcca cgtcagttgc ctcttccagg
     2941 tcggcgaaca tccctttaag tgccttagtt actgaaagtg cgcgggctcg ggtgaacacc
     3001 agttcatcga acagcaccat gtcggacgca tcatccatga ggttatctgc ctcaagatac
     3061 ttcgcatatc cacggtgtct tacggcgtgg gtgttacggt cgctaaaccg ggcaacgggg
     3121 tgaggattgc ccgatctgct gttgcgtttc gtttctgccg aatttgcgca gttttttgcg
     3181 catttttcac cactgccatc atcgctgtaa gccttgtcat tactggattc gtcatcggat
     3241 ccctgcacat ttttttgtgc gcttttttga tcatcaattt gcgcactttt ttgcgcattt
     3301 ttctgcgcac tttcctctgt atttatgggg gggtttttgc gcaattttat gtaccggcga
     3361 gccgtgccat aactgatatt gttcttacga caccagtcct gaagttttat gccagttctg
     3421 gcatgttcgc gtcggaatgc ctgctccagc tttttccagt ccagctttgc catgtcacgt
     3481 tctgacgtcc tgtgttaaaa actgatgcat aatgaccgct gtgatttttc agatttcaca
     3541 cagcagcacc atatttgatc gatatttgca caatgcggtt gttttatccg gtttcttcca
     3601 ccaccgcacc ggacaggcgg cttcgcggga aatcgctccc atctcgtgaa aaatgagaaa
     3661 acccggtgtg catcgttttt gattatcccc gcacactcac gcagataagg tggctgcggt
     3721 ctctgttaat gcgggaatac ggcgacgata cggcgcatgg ctatgtcagg ctgaaatgcc
     3781 tttatcaaat ccgggtaacc aatctgcccc tgctgctcca gcctgtccag ccttgccagc
     3841 aactgaggct tcttcaccct gccccagcga ttgagcaaac gacctgacat actggcgaca
     3901 tctttctctt tcatgtactc cagcattaca gcgttgcgct ccgcctccag attcgccaga
     3961 ccttgctgaa taagctcagc catccagttg aaagcctgaa tgtacgcttc cttaaaggtc
     4021 atagcagctt tccccgtaaa accaaatacc aacatcgtcc aaccgtcttt tgtcattcga
     4081 tatatgggct ggggtttacc gttctgtaac tcattgtttt catagcaaag cgcaaaattg
     4141 cgctctgcaa attcttttga gcattgttga acaacggctc ttgttttccg caatacatct
     4201 ttgtgttctt tgccaaatgc tacggcaacc tttcgggtat ccgtcaccgg ctcgttgcct
     4261 gtcacaaaaa caagatcgcg gaaatcaata ccgttaacaa tcgtcggata gttcatagcg
     4321 tcttaccttt cagaaaattg agcctgtctc acagaaaagc cgccccgaga tggtcgccac
     4381 catatacgac agttctcagg ctcaactttc tgaaaggctc ggggatgtga tatgcgcgtg
     4441 agatgcgcga tgaaattcag atatgaaaaa gccagcgatt aagctggctc tgttaattca
     4501 ggcactgagt gcggatatat tcctgtgccc cttccagctg cttctgcatt gtcatcaacc
     4561 gttctctgag gatgaaataa tcccgttcag cggtgtctgc cagtcggggc ctggttgcat
     4621 tatccacgcc ggaggtgccg gtggcttcac gcacggtacc ggagcaggtg gcgttgatcc
     4681 gcaggcgctt acgaccagcg gcaacatcag cacgcagagt ttcattttca gctctcgcat
     4741 cggctaattc cctcgagtat ctggcatcaa gtgcagcgac atcacgctgg cgtatctgca
     4801 tatcagtaat tgtcgcgttc gccagctcca gctcactggc ttttttatcg cgctgctctt
     4861 tgtagatgat ggcgtgaaca cggtaatgat tcagccccag actaagcgca ccacaggcca
     4921 ccagcaggac aatgatgacc acgcacagaa cacggttcat atcaccacca acggattgcc
     4981 cagaccagaa cagcaatggc cacaatacga atggcaaaag ctgccgctct tgttaaatcc
     5041 agactggctc tccacttcaa tgcctttcat aatggacaac ctcagaaaga atcttttata
     5101 cttcctcaca gggaaagtac ctccctaccc ataatttctc ccttgcctta ttcaaggtca
     5161 gaaaacacca aaccccgctt gctgccaaca accggggttt ttacttttat tcacttaggt
     5221 tttgccagtt cgcaggattt cgtgttatcc gcccgcgttg gccaatgtca tttttcagca
     5281 aaatattctg cttatctgtc aattccccag cacgccagcg cactttcctg atctcgccgg
     5341 gatacctgac cgtagcaatt atttgagcgg atacggcagt ctctgccacc gtccttaatc
     5401 caccagcgaa tcgcttcgca ggcacctttt cgatcacctg cattaattcg tttataaaac
     5461 gtcgacggga aacacttacc ggggccaatg ttatacggac agaatgacgc tatccccgct
     5521 ttctgggggt cggtcagcgg cactctgatg tttttctcca cccatgccag cgccttatca
     5581 cgttcaatgg cgttaacatg gtcgcatttt tccttcgaca gcttcatgcc aggaatcaca
     5641 ggcttaccat ccacccgggt ggctccacgg cagatggtcc agatacccgc accatcacgg
     5701 tatgccgtgg tgtggttacc ttccttttca tccagaaact ggtcgaggat ttcaggcgca
     5761 gaagcacctg cggcaatcag cgccagaacg gcagccgata aaccatagcg gagtttcctg
     5821 ctcatcagct tactctcccc gtgccgcctt acgcctgtcc tctctgattt tgaaatacag
     5881 gttcgtcagg tacgtcagca gaccaaacag cagactcccc agcacgccta ttgccgccca
     5941 ctgagacggg gaaaccctgt ccagcaactg caggaaccag tagcccgttc ccaccgctga
     6001 cgtggtgtat gacacacctg ttgtgatttt ttccatctgg tacatacccc gtctcccgca
     6061 atccggaagc tcacaacatg aaaaaggcca gcagctgttt actgatggcc ctgactcccc
     6121 gttacaacat catgaccgat tcgggttgag gttcagtcgc atcggcgacc ggtgattcag
     6181 gctgaacttc accgctctct gcggtggtat ctcccgcttc agtcggtggc tctgcctgta
     6241 caccaagcag ctcatccaga atggcatcaa cttctgcatc aagacgcgcc tcaagattct
     6301 ggcggagttt ctgtttcagt gcgctccgga cttcttcaga gcgcaggact tccttcactg
     6361 cctctgcagt gactagggat gtgatttctg acatgggatt ttctcgctga aaggggttgt
     6421 taaggagtaa cgggctcttc gggtttgctt ccggctgact gactggcgct gattttctca
     6481 gcggcccttt tatcaatctg cctgcaccag aaatcgcgca cagccctgta cccacccgaa
     6541 agaagataca gcacacagac cgccgtacag aaatacagca tgatttgatg aatgaatgac
     6601 ataatcacct cacacaaatg attgacataa cgatgtctgg cgttttaaaa aaatgaataa
     6661 caataataaa cactctccat aattcctctt acgctcccgg ctggtatcta ttcctctcag
     6721 tcttgcctgc cgggagcctc ttttttctta tgcggcgttg ctgttattga ttgccactgt
     6781 tacgtgctca atcagaaccg ggtaggtatc ctggttactg gtaatgtcag tgagtaacag
     6841 cttgtcgtta tccagtcctg ccgccgggca tttcgccaga ttaaaggccg cctgggtctg
     6901 accgttgatg accggtgtca cgctgatgct gttattgccg gcaaacctga acaccacgtt
     6961 gtgccagtta tgattaaatg ccccgaaggt tcccagtttt gcattctgtt gtttgtgctg
     7021 catcagattc aggtttgcgc tgtcgctctg gatgaagaag gcggccagca tatcgtttcc
     7081 tgcgctgcct tccagaacaa cgttttcagg tagctgagaa acaggccagt acagg
//
LOCUS       DAZGAN020000181         7120 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGAN020000181 DAZGAN020000000
VERSION     DAZGAN020000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.181"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(34..843)
                     /locus_tag="ACHKYO_RS23615"
                     /old_locus_tag="pgaptmp_004723"
     CDS             complement(34..843)
                     /locus_tag="ACHKYO_RS23615"
                     /old_locus_tag="pgaptmp_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU4431416.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAHDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(863..1252)
                     /locus_tag="ACHKYO_RS23620"
                     /old_locus_tag="pgaptmp_004724"
     CDS             complement(863..1252)
                     /locus_tag="ACHKYO_RS23620"
                     /old_locus_tag="pgaptmp_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4431417.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKTAGAAA"
     gene            complement(1249..1575)
                     /locus_tag="ACHKYO_RS23625"
                     /old_locus_tag="pgaptmp_004725"
     CDS             complement(1249..1575)
                     /locus_tag="ACHKYO_RS23625"
                     /old_locus_tag="pgaptmp_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HGU4431418.1"
                     /translation="MTTLTQCQQQVLDMLISYQQERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(1572..2225)
                     /locus_tag="ACHKYO_RS23630"
                     /old_locus_tag="pgaptmp_004726"
     CDS             complement(1572..2225)
                     /locus_tag="ACHKYO_RS23630"
                     /old_locus_tag="pgaptmp_004726"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU4431419.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(2225..2719)
                     /gene="yfdN"
                     /locus_tag="ACHKYO_RS23635"
                     /old_locus_tag="pgaptmp_004727"
     CDS             complement(2225..2719)
                     /gene="yfdN"
                     /locus_tag="ACHKYO_RS23635"
                     /old_locus_tag="pgaptmp_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4431420.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTEIPQEPEPQPVRESRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQQERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(2716..3657)
                     /locus_tag="ACHKYO_RS23640"
                     /old_locus_tag="pgaptmp_004728"
     CDS             complement(2716..3657)
                     /locus_tag="ACHKYO_RS23640"
                     /old_locus_tag="pgaptmp_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4431421.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDSSQP
                     DTQTAEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEHAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(3647..3826)
                     /locus_tag="ACHKYO_RS23645"
                     /old_locus_tag="pgaptmp_004729"
     CDS             complement(3647..3826)
                     /locus_tag="ACHKYO_RS23645"
                     /old_locus_tag="pgaptmp_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4431422.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(4002..4559)
                     /gene="ymfL"
                     /locus_tag="ACHKYO_RS23650"
                     /old_locus_tag="pgaptmp_004730"
     CDS             complement(4002..4559)
                     /gene="ymfL"
                     /locus_tag="ACHKYO_RS23650"
                     /old_locus_tag="pgaptmp_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HGU4431423.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(4603..4803)
                     /gene="ymfT"
                     /locus_tag="ACHKYO_RS23655"
                     /old_locus_tag="pgaptmp_004731"
     CDS             complement(4603..4803)
                     /gene="ymfT"
                     /locus_tag="ACHKYO_RS23655"
                     /old_locus_tag="pgaptmp_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HGU4431424.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            4894..5568
                     /gene="ymfK"
                     /locus_tag="ACHKYO_RS23660"
                     /old_locus_tag="pgaptmp_004732"
     CDS             4894..5568
                     /gene="ymfK"
                     /locus_tag="ACHKYO_RS23660"
                     /old_locus_tag="pgaptmp_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HGU4431425.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            complement(5783..6298)
                     /locus_tag="ACHKYO_RS23665"
                     /old_locus_tag="pgaptmp_004733"
     CDS             complement(5783..6298)
                     /locus_tag="ACHKYO_RS23665"
                     /old_locus_tag="pgaptmp_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431426.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGSYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVIVN
                     GGSITMHLCPKCFGQKIVSILQPFPVSEDELFHKSRCLHCENKFLMNKNPDYVSPPSI
                     EELSRKLNGNL"
     gene            6443..6754
                     /locus_tag="ACHKYO_RS23670"
                     /old_locus_tag="pgaptmp_004734"
                     /pseudo
     CDS             6443..6754
                     /locus_tag="ACHKYO_RS23670"
                     /old_locus_tag="pgaptmp_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6769..>7120
                     /gene="yfdP"
                     /locus_tag="ACHKYO_RS23675"
                     /old_locus_tag="pgaptmp_004735"
     CDS             6769..>7120
                     /gene="yfdP"
                     /locus_tag="ACHKYO_RS23675"
                     /old_locus_tag="pgaptmp_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HGU4431427.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRA"
BASE COUNT         1723 a         1909 c         1689 g         1799 t
ORIGIN      
        1 atttcagggg tcagtagtgc tctcacggtc acctcaatga acggtatcga gcagctttaa
       61 cagctcaggg aatcgggatt cgaagaaatg cggctgtgtc tcgcgcggat ttgcgggact
      121 ggtgatgttc ttgccgaaca tgcaaccttt cgctgtcagc gaccagaatt ttttgatgtt
      181 gttaatcgct gtacggctgt atcgttcgcg ctgctcgacg atccccagtt tcaccatctg
      241 gtgatatgcc tgattagccg tcaggcgtat accatactgt ttcagcagtg cactcagtga
      301 cagtgtcggg cgacttgagc catcgtgtgc atcagcagga gcatcaatgg catagcgcgg
      361 tgccagattc ggtaagccaa cagcctcctg gagtttctga caggcaccaa gcactgaaga
      421 gttagacagg tttaactccc ggcgcataaa gtccagcagg atcacgccag cctgcatctt
      481 gtcagcagcc tgtccggata atttttccgg tgcgctggtt accatatcga aagtacggat
      541 caccttcaga tggaatgacg ggctgatcca cattgcatag gcatacacca gttccttgca
      601 gacatacgtt ccccgttcat ttcccccatg aatcacactc accgggtcaa cacccaaatt
      661 ctgggtgttg gtcaattcat gaacaagctc aacagtttgt tggctggaaa gaaactttcc
      721 cggctccttg gttctggcat ttgcaccaga tgctactgct gcgcgatgca gatcgttcag
      781 gctgtaacgc ccataagcat cacgacgaac ttcaatacca tcaatgacca tcagattatt
      841 catacttcgt ttctcctctt gatcaggcgg ctgcacccgc cgttttctcg tacttactga
      901 tagtgatctc gaccttccct ttcgggataa ccggtcccca ctccaccagc attcttttca
      961 cctgactgtc gtcttcccac acacccgcat gggtcagggc gtcaaacagc gccttgttat
     1021 agttgtccag atcgcggatc cggttatccg gaggaaacaa cacgatctcc actgaagcag
     1081 gtgccgacgt tggtttcggc agacgacgta actgctcaac tattgctgcg cacgccgcgc
     1141 tctggaattt gcgccccgct gcgcttatca gactcttacc agcaaacgcc cctttgttgg
     1201 ggtgtcgcca gtacgtgttc acgctgggcg ggaaaggcaa gatcagcttc atactttcag
     1261 gcccctctca tgtaaccagt gggctgcacg cagcctggcg ttttcctcac cggcaagcag
     1321 tgcgcggata atcccgaccg cctcgctgtc gtcgtccttc accgcggtat gaagcgttat
     1381 cccccgggcc acgccacgct ttatcgtgat gacgcctttt ttctccagtg cgcgaagatg
     1441 ctccaccgct gcattcaccg aacggtatcc cagcatggtt gccacctcct gattggttgg
     1501 cggaaagcca cgttcttgct ggtaagaaat cagcatatcc agcacctgct gctggcattg
     1561 agttaacgtc gtcatgccgc catctccctg accagttttt ccgcctgctg gcgaacctgc
     1621 gccagaaagg cctcaccaca tgcctcaagt tcatcgcgcc cgatgtagct gattgccggt
     1681 cccttccagg tcttgtcgaa aacagcaata gcaccagcga agaaagcgcc tgtcggcacc
     1741 tgcttctcgt ccttcgggat aaaccaggct ggcagttcaa aaccaatacg cccgcgaata
     1801 aaagcaatat ggtctgcatc ttccggccac cacacttcgc tggtggcagc tttgatcagg
     1861 aaaacatagc gcccgccttt atcacgcatg gcactggcat gcttcatgat gtaacgcatg
     1921 ccggtgatgt attgcccctc atgctgactg gcgcggctgt acgggggatt accaaaggca
     1981 gcacctttaa gctccgcaag acgttctgac cagtcatgcg ccagcgcgtt gtcttccgca
     2041 gtgtaatacg cggcgcattt ggcgttatca ccatcagtaa acagatccag aacaaacggg
     2101 ccaaacaggg tgttaattcc ccagaaaatg ttgtccggcg tgcgccactg atcgcccact
     2161 tccttcagtt catgggctgg tttgttccgc agctccacca gcgcctggca atatttatta
     2221 ctcattaagc ccccacgtaa ttccctgaca gataccactc ttcacccgat gcagcgcgct
     2281 tgctgctttt ccgtaaacac cgctcacgac gcgcaagaaa attgtttcgc tcttgctggg
     2341 agtggctttc acggaatgcc gccatccaca ccgttgcagc acgacggtat aagcccctgg
     2401 actccagttc ttccgcctgg cgggtcaggc acaaaatcac ccggggatcg ttagtgccga
     2461 catagaaatt gcgcacaggt ctggattcac gaactggttg cggttccggc tcctgcggta
     2521 tctcagtcag ccgcgggaaa tgtctgcgtg tatccccttc acaacggtga gccacacgcc
     2581 cactctgacg taacttgctt gctgactgca gaacgcgctg tcgtgagtaa cctgcaaaag
     2641 catccgcaat gtctccggaa gtacaccccg gatgggcttc aatgaatttc tgaacgtcat
     2701 tcaaaagact catgatcacc ccctgaatcc tgccgggatc tggctgtagt ccacgttgtc
     2761 gtaactggct ttgaagtacg ggtcttcgcg tttttcggtg tacgtgctga cggacggcga
     2821 taagcgcagg gaaagctcat cccatttttc ccgcagcttc gacgggctga gcacgttacg
     2881 gcaccagaac ggatcgcgac tgacgcggct gtacatctcg cagatttgtt tatgagtacg
     2941 accatcctgc acacacatca ggcgaatttc gtttgcccag gctgtccagt tcggttcttt
     3001 gggacgaacc acctcgccgt cacattcggc ggcatgctcg tacagggcga tgattttttt
     3061 ccagagccac tgtgcgcagg tcaaatcatc ctgcgtcccc cactggcgct ttttagggct
     3121 gaatacaacc gcatcaggat ggcgagttaa aaaatcctgt tcagccgtct gcgtgtccgg
     3181 ttgcgaagag tccggacgag aaggtttttt atctgacgga tcatgttttg attttactga
     3241 cggatccccg ccagattctg acgggtgaaa acccgctttt ttgccagatt tcgacgcatc
     3301 aaattttgac gggtcagatt ttgatgcgtc agattttgac gggtcagaat ctgacagttg
     3361 agaaaatgcc gctgcctgaa gcttcgcaac gttaagctga taaacattcg acgcattgcg
     3421 gttaccctgg cgacgcgcct tacgcgttaa ccagccttct gcttccagcc gtgcgatagc
     3481 cgttctgacg gtactcatcc ccgcgccaat ctggcgggca atggtttcaa tcgatggcca
     3541 gcacacacct tcgtcattac tgaaatcagc caggcgggcc ataattgcca cgctggataa
     3601 tttcatgcct gacgctgcgc aaccatccca tacatagccg gttaatttag tgctcatgac
     3661 cgacctctat ttccctgaat ttacgacgaa actgttcgag cggactgaag cactcatgct
     3721 catagccttc gcggaggtag ataacccgtt gtgtttccgg ttcccaacga atgactctga
     3781 cgggcactcc gtagtgatct ttgaaccagc ggttaacttg tcgcaaagga ctgtctcctt
     3841 ctgccggttg aaatccccca cagcccactc tgcaaagctg tgggttacaa tttccctgtc
     3901 acctggtaca ttcactgcat agcaatattc caccttcgct tttccacccg gtacaggaag
     3961 cgcaatcagt tgcgagcgac ggtagtgtgt tgttaaactg ttcatgcgtt agtttctcca
     4021 caaccagaag caatcgacgc cacgacgccc ggagctgcac actcgcgggc gtcattactt
     4081 tctgaaatgc aaaagatttt gtagaccagt gctgcatgct cctgcagctt cgaaattgag
     4141 agatacagct cgtcgttaat tgctgtcttc tcatgcggtt ccactacacc gtcttcgatt
     4201 gctgaacgaa tctgttttga ataactgccg atctgttcaa tgacttccag cagacgctgg
     4261 ttaatatcgg cgttgtccac atcctcgacg tcaggaagcg acacaaagac gccatttgca
     4321 gactgcgcca cagcatcagc aatgaagtga gtgccaccag cacgctgtaa aaccattgcc
     4381 catcccagcg ggaaaatctg atcgccatct gcacgaaggc ggttgaataa agcgttttct
     4441 gttacatcga gccagtcagc cgcttcagcg taaccacccg gcaacgccgc gatagttttt
     4501 ctgacagctt tcacgtacca ctcaggctgt ttttctattt tccagtgatg cttacccacg
     4561 attagcctca tcgttctgtg gttaaaaatt gaaagtgttc tgctaatctt tcggatagat
     4621 atccggtctt aagtcagatt tcgtaattgc acctgacgtg cattgctcaa gttttttagc
     4681 cagcacaaaa ctggcttttt tatagccatt gaaaaccagc cgtaagtagc caggagttga
     4741 gccaactttt ccggccaact cgccctgctg ttctttggtt aaagagtccc aatacgcttt
     4801 catacaatat gtacctccgg tgtacatatt acatgattga aatgaacctt caagatactt
     4861 gtaccttaac ggtacaaggg ttttaatttc gttatgaaaa caatccatga catccggcgg
     4921 tctaacgcca gaaaactgag agatggtgtt ggagggaatt cttcctttgc cactatgatt
     4981 gatcgcgagc caacccagac cagcaggttt atgggagatg gtgctactaa aaatatcggt
     5041 gacagcatgg cacgacacat cgaaaaatgt ttcgacctgc ctgtcggatg gctcgatcaa
     5101 gaacaccaga caacgaacat cacaaaaaaa cctgatgttt caatcactaa taaacaaatc
     5161 acattagtcc ctgtcatatc atgggtacag gccggagcat ggaaagaagt tggatattct
     5221 gaggttgatt tgagcacagc agaaacgtat ccctgccctg taccctgtgg ggaaatgact
     5281 tatatcttgc gggtgatagg tgattcaatg attgatgagt accgcccggg agacatgatt
     5341 tttgtcgatc ctgaagtacc tgcctgccac ggtgacgacg ttattgcatt gatgcacgat
     5401 acaggtgaaa ccaccttcaa aaggttgata gaagatggga cacagcgtta tctcaaagcg
     5461 ttaaacccaa actggcctga gccttacatt aagatcaacg gtaattgctc tataattgga
     5521 actgtgattt tctcaggaaa accaagaaga tacaaaatca aagcctaatc aatgtttatg
     5581 aacctgcttc ggcaggtttt tttatacttg acaatgtacc tatgagatac ataatgtacc
     5641 caagagaaac aacaaacagg caggacgccc acgaagtagc cgcctggggc atatgaagtc
     5701 caggatgatt cgttagcaac aaaaaagcgc cctacaggac gcttagctct ttaacaatct
     5761 ggatatccac aacagtagta atctacagat tgccgttaag ttttctggac aactcctcaa
     5821 tggatggagg cgatacgtaa tccggatttt tattcatcag aaacttattt tcacagtgga
     5881 ggcacctgct tttatgaaaa agctcatctt cgctaaccgg gaatggttga agtatcgata
     5941 ctatcttttg tccaaaacat tttgggcaaa gatgcatggt tatgctgcca ccgttcacga
     6001 ttacctcctt cgagtataca aaagtacccg actcaagttg gttaaggata tagccttccg
     6061 tctgagcctc aaagttttcg aattctgcaa ttttagcttt gagagaagca tttatttctt
     6121 gataagagcc caccagttca acgagagaca cgcattcgcg ctgaatagac gcaagctttg
     6181 agttcagctc accaatagcc gcatttactt cagcttgagt ttttgcctcg ttcattagtt
     6241 ttgcaatctg ggctgtttca cgaatagccg tcattgctgc cgttaattca gcgatcacat
     6301 tgaatactct tattgttgtt ggggatatcc agattaaccg aatccttgtt gttggggaat
     6361 aaccaggtcc acctcgcctg atgtggctaa aagcaggcac ataacagcta agtattttca
     6421 accagagaga atccttagcg ttgtggtgaa tgcggctcag cgcacgcggg ttaaggttga
     6481 ggctgacagt cgaccttctg tggataccca cccgcctggt gtgcaacctt cgccaggcac
     6541 cgggaggcac ccggcaccac aagtttatgc tgtgtgtagt cctggcggta ccagtttgta
     6601 cccttgcttc cggctggtac cgtccttttt tgcaaaacag agaagagcat caccggacga
     6661 cgggctcata acccaatcca tccgggcggc tgccaccgca ggtgttcttc tctgttttgt
     6721 ggagaaacca accgaccttg cagggtcgat atgatgagga gcagcaaaat ggctagcgaa
     6781 cgcagtactg atgtgcaggc atttatcggg gagctggacg gcggcgtatt tgaaaccaaa
     6841 atcggcgcag ttctcagtga ggtcgcttcc ggtgtgatga acacgaaaac caaagggaag
     6901 gtctcactca atctggaaat cgaaccgttt gatgagaacc gtgtgaaaat caaacacaaa
     6961 ctctcatatg ttcgcccaac taaccgcggg aaaatttccg aagaagacac caccgaaacg
     7021 ccgatgtatg tcaatcgcgg tggtcgcctg actattctgc aggaagacca gggacaatta
     7081 ctgactcttg ccggtgaacc tgacggaaaa ctccgcgcag
//
LOCUS       DAZGAN020000182         6926 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGAN020000182 DAZGAN020000000
VERSION     DAZGAN020000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.182"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            118..921
                     /locus_tag="ACHKYO_RS23680"
                     /old_locus_tag="pgaptmp_004736"
                     /pseudo
     CDS             118..921
                     /locus_tag="ACHKYO_RS23680"
                     /old_locus_tag="pgaptmp_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(925..2316)
                     /gene="tcyP"
                     /locus_tag="ACHKYO_RS23685"
                     /old_locus_tag="pgaptmp_004737"
     CDS             complement(925..2316)
                     /gene="tcyP"
                     /locus_tag="ACHKYO_RS23685"
                     /old_locus_tag="pgaptmp_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HGU4431428.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLTVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(2449..3039)
                     /gene="ydjM"
                     /locus_tag="ACHKYO_RS23690"
                     /old_locus_tag="pgaptmp_004738"
     CDS             complement(2449..3039)
                     /gene="ydjM"
                     /locus_tag="ACHKYO_RS23690"
                     /old_locus_tag="pgaptmp_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4431429.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(3202..3870)
                     /gene="hxpB"
                     /locus_tag="ACHKYO_RS23695"
                     /old_locus_tag="pgaptmp_004739"
     CDS             complement(3202..3870)
                     /gene="hxpB"
                     /locus_tag="ACHKYO_RS23695"
                     /old_locus_tag="pgaptmp_004739"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HGU4431430.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            4017..4553
                     /gene="yniB"
                     /locus_tag="ACHKYO_RS23700"
                     /old_locus_tag="pgaptmp_004740"
     CDS             4017..4553
                     /gene="yniB"
                     /locus_tag="ACHKYO_RS23700"
                     /old_locus_tag="pgaptmp_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HGU4431431.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4594..5454)
                     /gene="yniA"
                     /locus_tag="ACHKYO_RS23705"
                     /old_locus_tag="pgaptmp_004741"
     CDS             complement(4594..5454)
                     /gene="yniA"
                     /locus_tag="ACHKYO_RS23705"
                     /old_locus_tag="pgaptmp_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HGU4431432.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5560..5850)
                     /gene="ghoS"
                     /locus_tag="ACHKYO_RS23710"
                     /old_locus_tag="pgaptmp_004742"
     CDS             complement(5560..5850)
                     /gene="ghoS"
                     /locus_tag="ACHKYO_RS23710"
                     /old_locus_tag="pgaptmp_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU4431433.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5951..6880)
                     /gene="pfkB"
                     /locus_tag="ACHKYO_RS23715"
                     /old_locus_tag="pgaptmp_004743"
     CDS             complement(5951..6880)
                     /gene="pfkB"
                     /locus_tag="ACHKYO_RS23715"
                     /old_locus_tag="pgaptmp_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HGU4431434.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLRSHDDTQKIYAYLSR"
BASE COUNT         1825 a         1670 c         1673 g         1758 t
ORIGIN      
        1 gctaatcatg atgagctatt tttgatagtt catcctgtgg cgtcgctctt aaacttatat
       61 tgtaagtgac ttagatttaa atgagataat aattttgtta acaatatggg gcgggagatg
      121 ttttctacaa acttaataca gagtaattat ggtgatttaa atattaaaag cctggctttc
      181 gactctttta aagaaaggct tgagtctacc atggcggcac tcactttctt tatatcaacg
      241 ggtcaatgtg actgcgatga aatagcagag tcgaacttca atcgtatgat tgcctatatg
      301 tctaacatta actatgatgc ctcaaagcca ggagcgccag cacttagttt tgatacgtat
      361 ctgcaggaca atgttaaata tagagtgata attaataatc tttatggcag tgagtttcgg
      421 ataagagata ttaatgagaa ttttgttggg atggatttta cctgtgaatt ctgacccgaa
      481 aaaatgacga atttgatcaa tataaaaata ggttggttat tcattattta aggactatat
      541 actatgaaaa aaattatata catcctgccg ggagcatttt tgctgcgatt caaaaaaaat
      601 gagagtctcc taaagtttcc ttcgataata tcaatgctta atatcaatct tctttttaat
      661 ccactaaatc ttccgggaat gggaagtggt gtgcttgaag atattatgag tattccagat
      721 agttcactta gaaaacgtct cgggtatgag gttttaagct tctcactaca ggcacattca
      781 ttgagccagg aatgtattga taagttggac atttttttcg ctgacgatct gtttaaatat
      841 gagtcagtat gcatcgccgc gatggaacat ttaaaaagta aagcaacggc ccctatacaa
      901 aacggaccgt tgccagcata agaattaacg gtgtgccagt tcggcgtcgt cttcgctatc
      961 cagaatggct ttatcggttt gcttcagcca ctggctggtc agcgtgccag ctgtcatcga
     1021 gccactaacg tttagcgccg tacggcccat gtcgataagc ggttcaacgg agattaacag
     1081 cgccaccagg gttactggca ggcccatcgc aggcagcaca atcagtgcgg cgaaagttgc
     1141 accaccaccg acaccggcaa cgcctgcgga actaacggta acaataccga ccagcgtcgc
     1201 aatccacatc gggtccagcg ggttaatgcc aaccgtaggc gcaaccatca ccgtcagcat
     1261 tgccggatac aaaccggcgc agccgttctg accaatggtt gcaccgaaag aggcggcgaa
     1321 actggcgatg gattcaggaa cgcccagacg acgcgtttgt gcttccacat tcagtgggat
     1381 agacgcagca ctggaacggc tggtaaaggc aaacgtcagc acaggccata ccttacggaa
     1441 gtacttcagc ggactcacgc cattaatgcc cagcagaatg ccatgcactg caaacataat
     1501 cagcagaccg aggtaggacg cgacaacgaa acttcccagt ttgatgatgt cttgcaagtt
     1561 agaacctgca accactttgg tcattagagc cagaacgccg taaggggtca attgcatgac
     1621 aaggcgaacc agtttcatca cccagctttg tagggtatcg atagcggcta agacgcgttc
     1681 acctttcggc gcatcatcct tcagcagttt caatgcagct acgccgagga atgcggcaaa
     1741 aattaccacg ctgataattg acgtcggatt ggctccggta agatcggcaa acgggttttt
     1801 cgggataaag gacaagacca gctgcggaac gctcagatca gagactttac caacatagtt
     1861 actttcaatg gcgttcagac gtgcagtttc tgcaccaccc tgaaccagac cttcagccgt
     1921 caaaccaaac aggttggtga ccagcacacc gaccagcgcc gcaatcagcg tggtaaacaa
     1981 aagcgtaccg atggtcagaa aactgatttt gcctaactga gatgcgttat gcagacgggc
     2041 aaccgcgctc agaatagagg cgaagactaa cggcataacg atcatttgca gcagttgaac
     2101 atagccgtta ccaacgatgt taaaccactg tacagaatct ttaagtacct ggctgtcaga
     2161 accataaatg gtatgcaggg caaggccaaa aaccacaccc atcaccagac ccaccagcac
     2221 ttttttcgcc agactccact gtttatggcg ggtctgagcc agcgcaaaca gcagtacaac
     2281 gaacaccacg atgttcgcaa ttaatggaaa gttcatcccc gttctcctga tcttattatt
     2341 tctatcactg cgtagcagtc atagtgttgg cgcaagatta acagaactgt gagctggctc
     2401 ttatattcaa atggaattgc ttattccaga aaggtgattt ttgtcttttt agtattcaac
     2461 ctgatgctta ataagccgat gaaactggat ctgcaaggta ttgatcattt gcgatgacca
     2521 acgaacagcg ctgttctcgg gtaatgaatg gggcatccat accgaccaga caaataatgc
     2581 catgcagata aaacgttcca gttggttgcc cttttgggga accaggatag gcaagcggaa
     2641 acgccagcgg catggccaga gcaggggaac accggcgggt gtcagcatat cggcaagtat
     2701 gtggctcaaa taacccagca ccattccttg tagcgcatca gccggaatga accagccttc
     2761 cggaacctta aggtaaaagg ttgccagcag cgcaaatacc gccagcagac tgtgggtaaa
     2821 accacgatgc ccaaaagcgc gggcgatcgg ttttgatatc cattttaatc gctgcccaag
     2881 aaacgacttt gggtgatcga tgtccggtaa caaacacgtc aggattgcgg aagggacaat
     2941 atgccaccag tcaccctgtg ccagcacggg cgtcagctcg gcatttttgg caaataccgc
     3001 acaagcaata gaaaagagaa ggtgaccttc cgccgtcatg ataaaactca ctaaactgtc
     3061 gatacgtaca gtataggatt tttatacagt gcacggaagt ggtgacggtg taactctttc
     3121 tcttaaattg acgattaatc cgtcagttga atgcaaattc agataaagaa ccaggggcag
     3181 cgagatcact gcccctggtc attaaccgag aaggtctttt gcggtgagtt ctgtcagcga
     3241 tgagagtttg acgtttgcta atacaaaacg tggatcattt tgcgcttctg gcgcaggaac
     3301 gacaatggaa cgcatgcggg ctgctttaga ggcgatcatg ccatttactg aatcttccag
     3361 cgctacgcag gtcagtgggt caacgcccag ttttgctgcg cagtcgagat atacttgcgg
     3421 atgcggcttg ctgtaaggca gtttttcagc cgaggcgagg gcatcgaaac tgtcgcgtaa
     3481 gtcaaacatg gtcaacactt tttccagcat atgtagtggt gacgcggagg ccagtcccac
     3541 caataaacct tgttctttgc ataacgcaac ggcttcgcgc acgcctggta ataatggacg
     3601 tgtctcttca accagtgaaa tggcacgggc aataacccgt tctactactt cctgacggct
     3661 tggcccattc cacggttgcc gggcgtacca aagatcgacc accatatcga tgcgtaaacc
     3721 taaggtgtcc ggcagctcgt tacgacggga gatatccacc cccaggcttg ccatcacatc
     3781 cagttcggct cgatcccata aaggttctga gtcgataagt aatccatcca tatcaaaaat
     3841 tgcagcaaga atctgacgcg gggttgacat tacaacctct cctttagagc attatgtgta
     3901 aggtattaag ccgtgcttat gattaagcgc ctatcatatc gcctttaaag aatgggcgaa
     3961 aacagatatc tacaggtaaa ctaagtgaaa cgacgcgtct taatcaagga gaactcatga
     4021 cgtatcaaca agctggacgc attgctgttt tgaaaaggat tttgggttgg gtgattttta
     4081 tacctgcatt gatctctaca ttgatttctt tgctgaaatt tatgaatacc cggcaggaaa
     4141 accaggaagg cattaatgcg gtcatgctcg actttactca tgtcatgatc gatatgatgc
     4201 aggcgaatac gccttttttg aatctgttct ggtataactc cccgacgccc aattttaatg
     4261 gtggcgtgaa cgttatgttc tgggtgattt tcatcctgat ttttgtcgga ctggcgttgc
     4321 aggattccgg tgcccggatg agtcgccagg cgcgcttctt gcgggaaggt gttgaagatc
     4381 aactcattct ggaaaaagcc aaaggggaag aggggctaac gcgtgaacaa attgagtccc
     4441 gtattgttgt tccgcaccat actattttcc tgcagttttt ctcactatat atcctgccgg
     4501 ttatttgtat tgctgcaggt tatgtgttct tttctctgct tgggtttatt taatcaaaag
     4561 gccgcacgga gcggccatta tcctcaaccc atatcatgct gctaataatc tatccaatga
     4621 ctgctgagca ataaccaaat gctgaccgcc aaataatctt gcacgattta acagcgtgta
     4681 gagttggtaa accggttgac gttcaaggaa atcggcaggt agcggggata ctgactgata
     4741 gccgtcatag atttgtggcg gctgttcagt atgcagcggt aacatcgcca ggtcgcactc
     4801 tcggtcaccc cagtagcagg cagggtcgaa aatgtacggg ccatccggac ccagcgcaca
     4861 gttgccggac cataaatcgc cgtgcaacag agaaggctgc ggttgatgcg aggccagacg
     4921 ctgctgaata tgctcgacga tggcgtcgat attgccgaaa gcgatccctt tctctgctgc
     4981 cagttccaac tgccagccaa tccgttgttc agcaaaaaac gttgaccagc gacgttgcca
     5041 ggtgttgggc tgtggagttg tggagagcga gttatcaaaa tcgaggccaa attgtggttg
     5101 gtcactccat tgatgtaaac gcgcaatttg ctgaccaaga ataaatgcgc tatgcgcatc
     5161 cagcggacga ggtgggagat aatccatcac cagaaaactg tagtcacggt cagcgcctac
     5221 tgcccaaacc ttaggcacgg tgacggtttt actacgcgac agtaactcca gttggtcggc
     5281 ttcggcggta aaaccgggaa gcagttccct ttcatcacat ttgacgaaaa agtcatgtcc
     5341 tgcatagcgc aaatgccatg cggcatggac ttctccgcca ggcagttcat tacgcagttc
     5401 gatttcgcct tcacctaact gctcgctcaa aagacgactg attgcctgcc acatgatgtc
     5461 tctcccatgt tgtctgccag atcataaggt tagcgcaaga atgcggtgta aaaacacgag
     5521 ctaacgcaca ccacagactg attattgccc aaaaaccatt tattttctgt tgctattcca
     5581 ttcctcccag gtcgtgatgg tgatttcagg atctttgccg gttgcacttt gggtaagccc
     5641 cgaaaccagt tctctaattt cttcttcact ttgggtactg ataagtccaa aagtgttagt
     5701 ccccagctca tggatatttc cctcatcatc cgtcatggtg agcaaaaaac cgtcgcgagt
     5761 caggtaatta ttcaactcgt taatttcagt caatgtatcc tcatgcaaca tgacggttat
     5821 gacataacgg acaagatcgc cactggccat agttcacccc tttgttatca catgactttt
     5881 gagcatagtc ggagaaacgc gttgccgaca ggttggtggt gattccccca atgctggggg
     5941 aatgtttttg ttagcgggaa aggtaagcgt aaattttttg cgtatcgtca tgggagcgca
     6001 gacgtgttcc ctgattgagt gtggctgcac tccccgcagc tacgccaaaa cggaccatct
     6061 cttcaagaga ggcattttct gccagtttca gtgtcatcgc gccgaccatg ctgtcaccag
     6121 cgccaacggt actctggctt ttcaccggtg gtggcaccac ctgaatgcag ttttcactat
     6181 caacacccag cgctccttgt ggaccaaggg aaacgacaac ccgtttggcc ttgccgctat
     6241 taacgatttc ctgcgcggct ttgcggacat cgtctggctg ggtgagttcg cgattcacca
     6301 gcgcactgag ttctttttgg ttaggcttaa ccaactcgat gttaccaatc gccagtgctg
     6361 cacttaacgc ttcgccagaa ctgtcgatga tgcagcggat cccttggttt tgcgcagcgg
     6421 aaatcagttg ggttaatttt tccagcttca cacctggcgg caggcttccg cttatgacca
     6481 ggatggcccc ggattcaatt tccagaactt gctcttcaag ctggcgaaac tcatcttcat
     6541 ttaatgccgc gcctggcata acaaaacgat actgctcacc gcttgcttcc acatgtacgt
     6601 gtaaattctg ccgggtccag tctttggctt ctacagtagc gacggggaca ttttcatccg
     6661 ccaacagaga aaccaggtgt tcgccggtcg cgccacccgc cgggaagatc gctgtggcac
     6721 tgcctccaag atgggcaatg gcgcgggcga cgttgatgcc gccgcccccg ggttcgaaca
     6781 ccggtgcggt acagcgcagt tttccttcgg gataaatttg cggggtaatt gttgcgctat
     6841 cgagagaggg cgcaagtgtc aacgtataga tacgtaccat catttcctcc tataggctga
     6901 tttcagtctg gcaccgaatc agccga
//
LOCUS       DAZGAN020000183         6685 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGAN020000183 DAZGAN020000000
VERSION     DAZGAN020000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.183"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..3927)
                     /locus_tag="ACHKYO_RS23720"
                     /old_locus_tag="pgaptmp_004744"
     CDS             complement(<1..3927)
                     /locus_tag="ACHKYO_RS23720"
                     /old_locus_tag="pgaptmp_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HGU4431435.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKEVDFPEAGEDSALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLRFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTDDDRLAHSPADRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVTVTGAEGYYNATLDYGDGCTTLTDGKGTHRYYYDPD
                     GNILREAAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADSAVWQ
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     IERAGTGAEGFRYDRHGNLLAWTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYILDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEIILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLVREYRYDSA
                     GNVSAVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSR
                     DQLTGYCSAQGELWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAE"
     gene            complement(3947..4408)
                     /locus_tag="ACHKYO_RS23725"
                     /old_locus_tag="pgaptmp_004745"
     CDS             complement(3947..4408)
                     /locus_tag="ACHKYO_RS23725"
                     /old_locus_tag="pgaptmp_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HGU4431436.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(4436..6321)
                     /gene="tssI"
                     /locus_tag="ACHKYO_RS23730"
                     /old_locus_tag="pgaptmp_004746"
                     /pseudo
     CDS             complement(4436..6321)
                     /gene="tssI"
                     /locus_tag="ACHKYO_RS23730"
                     /old_locus_tag="pgaptmp_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
BASE COUNT         1244 a         2007 c         1837 g         1597 t
ORIGIN      
        1 ttccgcaata ctgtcgccgt cccacaggta cgtgaagcgg attttcttcc ggtcgcagcg
       61 tttctctgtt cgtctgccgc tggcgtcgtg gcggtattcc cacagctccc cctgtgcgct
      121 gcaatacccg gtcagctggt cccggctgtc ccaccggaac cgctctgttt ccgggcggta
      181 gccgtcacgg tgttttgtcc ggctgaccat ccggcctgcg gcgtcatagt catactgcgt
      241 gtttccggcc tgtttcagcc ggtggcctcc ggcatactgg tcagtctctt cacttatccg
      301 gtgcctgccc gcagactgcg ccttcaggta gccacaactg tcatagccat aggactcgtc
      361 gccttcgcca tagcccagcc cggtgcctga ggctgtcacc gccgtgacct ggccgttccg
      421 gtccagccgg tactcccgcc gtgtttcccg tccgtaatct tcccggctgg tgacagccga
      481 cacattaccc gcgctgtcgt aacggtactc ccgtaccagt gaggggagca gcccgcccca
      541 tgtggcttct gccgggaaaa gtccggcgcg ctggctgaca ggctgaccgg ctgcattcca
      601 gccctgttca caggcaaaac cccggttact gtgccggagc acttcacgtc ccgcctcatc
      661 acggctgaac gtcagcgtct catcccccgg caggataatc tccgtcagtt ctccggcggc
      721 gttgtagcgg tacgtggttt tcagcaggga ttcatcgggg tgtctgtcgc gttcatcttc
      781 cggtggcagg atacagcgga tgacggtgtg ttcgtcgggg tagtggtatt caatggcccg
      841 tcggccctga gtggtgacat tcacccgccc gcgttcgtcg tagccggtgc gctgtgatgt
      901 gccgtccggg ctgaagatac ccgtcagcct gccggatttg tcataggcat actgcgtttt
      961 gccttccggg gtgatgactt ctgccaggcg gcctgcggcg tcccggctgt agaggatggt
     1021 ttcctccagg ccgttgaccc tggcggtaca caggccatcg gcgtcatagc ggtaacgcca
     1081 gaccacgccg ccccagtcac gctcttccgt cacccgtcca cagttgtcca gaatataacg
     1141 gtatgactcg ccctgcggat tgatgacttc tgtcagttgc agcgtgtctt tatcgtagcg
     1201 gtactgccag cggtggcctt caccgtccgt tcgcgccacc ggcaaatcga acgggccgta
     1261 ttccatcgtc cagacaacgc cgttgccgtc cgtccaggcc agcaggttgc cgtggcggtc
     1321 atagcgaaat ccttccgcac cggttccagc acgttcaatg ccgctcagtt tatggtgctg
     1381 ctgccactgt aaccgcactg actgaccgcc ggggccgcgc atcttcaccg gcaggtcgtg
     1441 accactgtat tcggtgtggt gttcacggcc catcacgtcg gtgagtgttt ccggccggtt
     1501 gtggtggtcg tactctatgc ccagacgccg ttcatcctgg cggaatatcc ccgacagcag
     1561 cccctgcggc gtgtacgcca gccgggtctc ttcgccctgt ggcgacacca ggcttaccgg
     1621 caggccgcgt tcatcacact gctgtgtcca cgtctgcccg gctgggtcgg tgatgttgct
     1681 gagcaggccg cgctcatcat agcggtactg ccacaccgcg ctgtctgccg ccgtataacg
     1741 gcttaactga ccgtgtgcgg cgttgtattc aaacttctcc acccgcccgg caggggagtg
     1801 gcgggccagc aggtgatgga attcatccca ttcatacgtg gtggtgctgc cgtccggcgc
     1861 tgcttcccgc agaatattgc cgtcaggatc atagtaataa cggtgagtgc ccttgccgtc
     1921 cgtcagggtg gtgcagccgt caccatagtc cagcgtggcg ttgtaatagc cctccgcccc
     1981 ggtgacggtc acgcaccggc cctgttcatc gtaggtgaaa cggctccacg tctggtcgtt
     2041 atcggaccag cggatgatcc ggtccgcagc gtcgtactca taaaacaggt ggtagtccag
     2101 ccgcgcatcc gcttcggtca gccgcccccg cgcatcctgt tcgtaggtcg ccagcgtctg
     2161 tatgccgtta tccgtgcggt gaatggcttt caggtagccg ctggcatggt acgtcagttt
     2221 aagccggatc ccgtcggtgt gactgacacc cgtgatccgg tactggctgt cgcgctcaaa
     2281 cgtgatgcgg ttgctggcgg tgtcgctcat cccgcccagc atgtacatcc ggtctgcggg
     2341 gctgtgtgcc agacggtcat cgtcggtatc gtcagtcacc gggtggcgca ggcgcattcc
     2401 cggaaaggcg tcataaaaga agcggctgct cagggcatcc ctgtcgaaca gctccaggta
     2461 atcatcgcgg cggcgcagaa tgtactgggc gtgatacggg ttaaccgccg tatcactgtc
     2521 accgtcgaag gcaaatctca gcgtggctcc ctggctgatg atatccaccc ggtttccctg
     2581 ttcacgcacc caggcgtatt cgctccagga gtcgctccag cccacgcccg tcaggccatg
     2641 cagtggcagt acagaactgt ggaaacgggt gaaggagagg gggagggtct ggccaagggt
     2701 gaagtcggta cgttcgtcgc agtatgcgcc ggttaccgga tcgacaggat ctgttaccca
     2761 tctgatgact ttattggcaa tactttttaa cgacttacct gttttcccca gcaacgcgcc
     2821 gagctttgcg cccagcagct tcccgctttt ccccagcagg tttcccagtt tgccgagtct
     2881 cgccagattc ttcagttttc ccagcagtcc cgctggcggc agaagatagc tggctatcgt
     2941 tattcctccc aggatcattc tctgccatgg cgggaactcc ggctggatat ccagataggt
     3001 gacctgctca cccccaataa agacgtccgg cgaaccttcc acgattgccg cgctgcactc
     3061 cagtttgtcg cccttgcgcg cggcaggctg attgttgata aagacgttgc tgctgccctg
     3121 ggctatcctc ggtggtttgt tctccttgtc gcagagcgcc gagtcctcac cggcttccgg
     3181 gaaatccacc tcttttttga tttcgccacg cccgcccacc atggtggcaa actccattaa
     3241 taccggaccg gcgggaaaca gaagcgatgc gccggcgctg acgcgatcca taaaaccggc
     3301 attgctgtcc gtcagggtga taacagcgtt cccccaggcc ttcgccagtg gtactgcttt
     3361 ttccgctgcc gattcaagca ggtcagtggc aatatcaccc catgagggtt ccccctgtgg
     3421 gacttcaggt attgctccag gtgggggagg agggggaagg gtgacggcgg ccctggcagc
     3481 gggctttccg ttaatgttta cattttcaga accggtattg atggcgccgt ccgtgtcaca
     3541 aaagctgttt atccatgact caagctggtt ctgtttctcc tccacataat cactcgcctt
     3601 atagctggca tacatcagcg caggcaccag aaccagcgca taagggccgg tggcgcaggc
     3661 ggccataatc aggcaacctt ctgcgatggt cactatcgcc cccagcacgg cccctgccag
     3721 tgcccccaga aaacttttat gctgaatttc atcgcccagc ctggcggcag gtgggccgga
     3781 cttctttgct gccagcatct gtgcaatggc tttgtcgttg gctgcccgtt tcatctggct
     3841 ggcgaccatc gcctgctgtg acagcattcg cattgccagc gtaccgcctg cggtcgctcc
     3901 ctgtggcccg cctggtcctt cactcatgtg ttatccctcg tttatgtcag cgcggctggt
     3961 ccttttggat gtcattctcc ggtggcaacg gggtgaagct ctttatcacc tccagcagca
     4021 tcgtcttttg actgttcttc agggataagg aggtgctgaa agtgaaggtc agcagcgtat
     4081 ctccacgccg ctggaatacg gctatctggc taatccgccc ctgattcccc tcatcttccg
     4141 gaccgttcca ggaataatcc agcagccagg ccggggcctc tgccagagtg atttcccggc
     4201 aggcatgttc ggtgtaccca tcaagtttgc cctggtacag cgccagcgtt tcctggtaca
     4261 gctctggtgt cagggttccc tgtggaacgg cttcgcgggt aatgaccagg ttgatgccac
     4321 tgttatctgt ggacaccagg acctgcattg actgatcctg ccattgagac ggcagttgaa
     4381 tgtggccttc attaaaagtg aactgcataa aatactccct gataatctga gtgaatcagt
     4441 tgatgaaaac agaaccgccc acgatggtga tgccatcctt cgacagcctg attaccgtcg
     4501 attccccgac ctgtagctga atgccatccg gcgtcagcgc aattttgctt tcctccacag
     4561 ccagcaaaat ttccttaatc gccgtataag cctgtatacc gtcgccttcg ccaccattgt
     4621 ttacagcgtc aacatgcacc gaaccggcaa tcgtgcctct gtcctgaaag acatcctcag
     4681 ataaagaccc tttcagtact ccggtgatct tgtggccttt ttccaccgta agaaggcggt
     4741 taccttcctg gatcgtgacc gtctggtcat tttttacggt ttcgcggtga tcgtgtttta
     4801 cggtggtggt acggtcgttg agcacttcgg tatccatgtt cttctgcgcg tggatgtaga
     4861 cctgttctct ccctgtcgca tcatcaaact tcagctcatt aaatccgctg cccatgtacg
     4921 ttttggagcg gatggtcatc tgcgtctttg ttcccggcag gctgccgggg gtgcggtttt
     4981 cctggtggta ggtgcgcccc ataatgatcg gctggtccgg atcgccgttg aggaagtcta
     5041 caatcacctc ctgacccaca cgcggtatcg ccaggtggcc aaaaccggtg cctgcccacg
     5101 cctgtgccac acggatccag cacgaactgt cttggtctgc cgggttataa cggtcccagt
     5161 tgaacttcac ccgcacgaga agatttcctc gcctgccggt cctgtcacga cagcgctctg
     5221 cgggccgtcc accagcggtt tcagcaacgg ctgtggtcgc catgttctgt ctgccgggat
     5281 caccgcaaag tgattattca gcgtggtgcc tgaaccactg cgtcccggca ccgcctgtgg
     5341 ctgttcgccg tgcagttcac ttgccaccac ctgccattcc cggttcaggt tcgcctgcgg
     5401 atgccccgtc agcacaattc gccgtcccgg ccatatctcc ggcgagcggc tcattccccg
     5461 cgcggtttct gcgttgtttc gccagccgtc catctgccag cgggcaaagt tctgcccgtg
     5521 ggcgctcttg aaacgtccgg ggtagtcata cacttcatac tgcgtgcgct ggtagtcctg
     5581 gtgctggcct tcctgttcaa aacgtccggc ccagccgggg cgtttaaagg tgtagtcttt
     5641 ggtcaccacg gaagaagggc ggatttgtgc gctgtagcgg aactggctga tgcagagggt
     5701 gctcacctcg gtacgggtgt tcgggttcca tgggatttca aaagattcgg gcagatggcg
     5761 gactgtgtcg cacagcacca ggctctggtc ggtacttttg taagcatgct cctcataaaa
     5821 gaagatgcct tcctccgccg ccatccggca caggaaatcg taatcagtct caccgtactg
     5881 gacacaaaac tcacgggaag gatgcggctc gctgaacagc ggactccatt cggtcacccc
     5941 gttttcctgc aacatcgtgc cgaggatgct tttgatgtcc tcgttctgga agatacggaa
     6001 gttctggcgc agaccggcac gccacagcgg cgggtgcacc ttcatgctgt acagcatctg
     6061 gtttttgtcg ttctccccga gttcaaacca ggtcaccacg cctttcaccc ggcgctgtac
     6121 ttcatcgccc tgccatatcg tcaggtaggc cattttgtcc agcacctgcg caaattcaag
     6181 ggagagaaac tgctggctga ccagggagag atcgagggaa aagagcgaag agagtgactg
     6241 gttcagatga aaggaaacca ccgcaaaagc atccggcggc aggccgtcca cttccagtgt
     6301 gaaacgtaat ccggttgaca tagcaacctc cagtaaagtg aataatgatc cgtaccgtac
     6361 aactgcggat aatgtgataa gcgggacgac ttcagacgtt attagggaag gaacagaaaa
     6421 tgctatggtt atgtgagaat tttgtttaaa tattacttta cagaaacggg tgtctgttca
     6481 gtcaaaaaaa taatcacaag ctcaacctct tgtttcttgg cgttattcag gacaggtacg
     6541 catatcacaa cgagtaacaa aaacgtaaag ttaaaccaat attcataata tatttttatt
     6601 cacagcaata aataatccaa ttaacattat taaaatgaat gattgcataa gaaatatatg
     6661 ctaccgctcc accctgataa catga
//
LOCUS       DAZGAN020000184         6678 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGAN020000184 DAZGAN020000000
VERSION     DAZGAN020000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.184"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            10..249
                     /locus_tag="ACHKYO_RS23735"
                     /old_locus_tag="pgaptmp_004747"
     CDS             10..249
                     /locus_tag="ACHKYO_RS23735"
                     /old_locus_tag="pgaptmp_004747"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4431437.1"
                     /translation="MKRKLQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAIRCGYA
                     STPYFISVFREYFGITPHQMMINQSGFIESRIAIK"
     gene            1162..5253
                     /gene="espI"
                     /locus_tag="ACHKYO_RS23740"
                     /old_locus_tag="pgaptmp_004748"
     CDS             1162..5253
                     /gene="espI"
                     /locus_tag="ACHKYO_RS23740"
                     /old_locus_tag="pgaptmp_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HGU4431438.1"
                     /translation="MNKIYALKYSSLTGGLIAVSELSKKVTGKTGRRLMTVSLVLSVT
                     LSALPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEINDNNGNKVGVLDVPMLDFS
                     SLSRDGHTTLIHPGYVVSAKHGGLQSVSSATFGYDQIYKIVDNNLAGIDFSAPRLNKL
                     VTEVIPADIQGKDKFNNNRYTAFYRAGVGSQYIRYANGTDKLLQAYTPEKAYLTGGTV
                     GKPYYTHYNGMKMISANPGNTFDKNQGPLASYGQSGDSGSPLYAWDNIDKKWVLAGVT
                     LHNYGVKGARNDWLLIPHDFISQKLQDDLKPIIVASPEENILRWEFDRSRGTGTLSQG
                     EKIFSMTGSVNGNANTGNNLVFSGNEGKIELVSSVEQGAGYLQFDKDYTVLTNNNSTW
                     TGAGIIVGDEANVKWGVNGIAGDNLHKVGSGTLTVNGHGENKGGLKVGDGVVVLEQQP
                     DANQKQQAFSHINIASGRATVKLNGANQVDADNISWGYRGGKLDLNGYDFTFSRLQAA
                     DYGAEISNDNQTDKSIVTLSLSPLKAEEINVVVNNINIMGGTGKPGDLYYTTFDGNYY
                     LLKSNRYGSALFGALNNQSEWQRLGKDKEKAIGLYTQMKMQESAPLSYIYHGKITGNT
                     SVEIPKLAGNDILTLDGSVSISGDMSKQDGALIFQGHPVIHAGQTVSASQSDWENREF
                     SLNNLNLNNADFSLSRNAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKS
                     AAAGTSSYTGHITLEQKSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLN
                     IHKGGALTAQGGLFTSGSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGFKLMSDSS
                     VLKARDQASVVGDIISDKQATISFGTESGKEGMLSEKASRGLAVGLLSGFNTAYRGAI
                     HAPSASATMNNTWWQLTGDSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAY
                     AMRTDLKNADKLVVNQKLSGKDNILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSE
                     QAIGFSNVTPVITAKDAGDKTTWNLTGYRMAENPAATQSASGLASVGYKSFLSEVNNL
                     NKRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNHTHVQVGVDKKHELDGLDLFTGFT
                     VTHTDSSASADAFKGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGI
                     RDYSTHSWYAGAEAGYRCHVTEDTWIEPQAELVYGAVSGKQFAWKDQGMHLSMKDRDY
                     NPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSR
                     MLMSVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            5745..6110
                     /locus_tag="ACHKYO_RS23745"
                     /old_locus_tag="pgaptmp_004749"
     CDS             5745..6110
                     /locus_tag="ACHKYO_RS23745"
                     /old_locus_tag="pgaptmp_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431439.1"
                     /translation="MNDGRQTPPSPEAWKIRFLANGTPGTWFYTDDAAVTKEWLRAHC
                     DDVEVIPLCRMHDGPVFREHHINELSAALARMLHMHHIQLERTRYRENIHDAECIREI
                     HEAPLQAPGILEKYKRTKS"
     gene            6120..6311
                     /locus_tag="ACHKYO_RS23750"
                     /old_locus_tag="pgaptmp_004750"
     CDS             6120..6311
                     /locus_tag="ACHKYO_RS23750"
                     /old_locus_tag="pgaptmp_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431440.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFSAL
                     KKENVGEFALRGVCVPGGE"
     gene            complement(6462..>6678)
                     /locus_tag="ACHKYO_RS23755"
                     /old_locus_tag="pgaptmp_004751"
     CDS             complement(6462..>6678)
                     /locus_tag="ACHKYO_RS23755"
                     /old_locus_tag="pgaptmp_004751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431441.1"
                     /translation="YPSLSLILRYIHCLAFQGAGSEEYFVCASLPDYPAHLFMHPDTF
                     NTEVDFSNCQVFVESQRGSMICMLPQY"
BASE COUNT         1946 a         1407 c         1581 g         1744 t
ORIGIN      
        1 gagagtttac tgaaaagaaa acttcaggag gaaaaagttt ctttctccca aatcctgctg
       61 gatatacgca tgcataacgc ccgacaactt atttccagta aactgtcagt gaatcaaatc
      121 gcaatacgtt gcggttatgc cagcacaccg tattttatca gcgttttcag agaatatttt
      181 ggtataacac ctcatcagat gatgattaat cagtccggat ttattgagag cagaatcgca
      241 attaagtaaa gtatgtcagt cagcgcataa taaaagtatt cttatgcttc attatcagtt
      301 tctgtttatc actccggagc actgtagtaa ctatggaccc ggaaaacaga tggtaatgag
      361 ttgactgact gatgatattg tggcgacctt tttaaggatg cgagcaaaga atgtattggt
      421 ccgtttgatt atgcagtgat taacaaaact ccgggaagat gaatgagtat acctctcctg
      481 caatccgttt cctgagaaaa caggtggtaa cacaatattc ttaatatgtt actgtttcac
      541 cacacaacca ggtttacggt tgtgtggtga actatagtaa tcccgggtaa caatccggag
      601 ctgttccttt tatactcttc agactgtgta ctcatccacg ccatccgttt ttcagtctca
      661 gtgtgtgaat aataaattac actttgaagt gaacgccagt caggaccatg tcgtatttcc
      721 ggatggatga gcatatatca gaaactacaa ttactacaga taacggtttt cagtaattct
      781 gtcataactt gccggaatgt aatgttttgt gacatggtcc ttatttttta tacacaggca
      841 ggttttgtcg ggatggaatg gataatgaac tcttatcatt ataaggcggt aacctctttt
      901 ttatgttatt aactttcatt attaccaatt ctgtttatcg attcagttct ggaaatacag
      961 aaaaattcag tgcaggtgta taaatcatca ccatgcgaaa tacataaaat atattaatgt
     1021 ttgttttcca caggaaaaat cttaagtatg gacaggtatt cctgatgtca tttaaaatag
     1081 attcagggtg agtatccagt actcactctt tccggacaga caatacattt taataccata
     1141 taattatata gcaggaatta tatgaataaa atatacgcgc ttaaatatag ctcccttact
     1201 ggtgggctta tagctgtgtc agaattaagt aagaaggtca caggaaaaac cggcagaaga
     1261 ttaatgacgg tttccctggt attatcagtg actctttctg ctttaccggg taaagcatca
     1321 acggttagcg cagaaatacc atatcagact tttcgtgact ttgctgaaaa taaaggtgtg
     1381 tttacccccg gagtgacagg aattgaaata aacgacaaca atggaaataa agttggggtt
     1441 cttgatgttc ccatgcttga tttttccagt ctttctcgtg atggtcatac cacattgatt
     1501 catcctggtt atgttgtatc ggctaaacat ggtggtttac aaagtgtttc atcagcaact
     1561 tttggttatg accagatata taaaatagtt gataataacc ttgctggtat agatttttct
     1621 gccccacgat taaataagct tgttacagaa gtaattcccg cagatataca gggaaaggat
     1681 aaatttaata ataaccggta tacggctttt taccgtgcgg gcgttggctc tcaatatatc
     1741 cgttatgcaa atggcacaga taaactactg caggcttaca ctccagaaaa ggcttatctg
     1801 accggcggaa cagtggggaa accttattat actcactata acggtatgaa gatgatttca
     1861 gcaaacccgg gaaatacctt tgataaaaac cagggacctc ttgccagtta tggacagagt
     1921 ggagacagtg gttcgccgtt atatgcctgg gataacattg acaaaaaatg ggtattagct
     1981 ggagttactc tgcataatta tggagtaaaa ggtgcacgaa atgactggct cctgatacct
     2041 catgacttta tcagtcaaaa attacaggat gaccttaaac caattattgt tgcttctccg
     2101 gaggagaata tcttacgctg ggaatttgat cgttcgagag gtacaggtac tctcagtcag
     2161 ggagagaaga ttttttccat gactggtagt gtaaacggaa atgcaaatac cgggaacaat
     2221 cttgtgttct caggtaatga agggaaaatc gaactggtat ccagtgtgga gcagggagcc
     2281 ggatatcttc agtttgataa agactacact gtactgacaa ataataacag tacatggact
     2341 ggtgccggaa ttattgtcgg tgacgaggca aatgtcaaat ggggagttaa tggcattgcc
     2401 ggtgataatc tgcataaagt tggttccggg acattaactg ttaatggtca tggtgagaat
     2461 aaaggtggcc ttaaagttgg ggacggcgtt gttgttcttg agcaacagcc agatgcaaac
     2521 cagaaacaac aggcgtttag ccatatcaat atagccagtg gtcgggcaac agttaaactt
     2581 aacggggcaa accaggtaga tgcagataat atcagctggg gatatcgggg tgggaaactg
     2641 gatttaaatg ggtatgattt taccttttcc cgtcttcagg ctgcagatta tggtgctgaa
     2701 atcagcaacg ataatcagac agataaatcc atagtcacac tttcgttatc tcctctgaaa
     2761 gcagaagaaa taaatgtggt tgttaataat ataaatataa tgggggggac aggcaaacca
     2821 ggtgatctgt attatacgac ctttgacgga aattattatc tgttgaaaag taaccgatat
     2881 ggcagcgctt tgtttggcgc gctgaataat cagagcgaat ggcaaaggct gggtaaggat
     2941 aaagaaaaag caattgggtt atatactcag atgaaaatgc aggaaagcgc tcctttatca
     3001 tatatatatc atggaaaaat aaccggtaat accagtgtgg aaatccccaa actggcaggc
     3061 aatgatattt taacgcttga tggctctgtc agtatatcag gagatatgtc aaaacaggac
     3121 ggtgctctta tcttccaggg acacccggtt attcatgcag ggcaaactgt ttctgcatcg
     3181 cagagtgact gggagaacag ggagttctca ctcaacaatc tgaatcttaa taatgcggac
     3241 ttcagtctgt cccgtaatgc atttatgaac gggaatatca gggccgttaa ccagagcact
     3301 gttattatcg gcggagatac agtctttact gataaaaatg acggaacagg taatgatgtc
     3361 atcagtgttg aagggaaatc tgctgccgca ggaacatcct cctatacagg gcatatcact
     3421 ctggagcaaa aatcagcact ggatatccgc gataattttc gtggcggggt tacgtctgaa
     3481 gacagtcata tcaatgtttc ttcatcttca gtcctgttct cagatgcatc gtcatttata
     3541 aacagctccc tgaatattca taaaggaggt gcgctgaccg ctcagggagg gctgtttaca
     3601 agtggaagca ttgatattgg tgacgcttcc cttctgctta ccggtacacc agtgaattca
     3661 gatgatgctg cttttttacc gaccatcaat atggctgatg gcggatttaa actgatgtct
     3721 gattcatcag tactgaaagc cagagaccag gcatctgttg ttggtgatat tatttctgat
     3781 aaacaggcca caatcagctt cggaactgaa tcaggtaaag agggcatgtt gtctgagaag
     3841 gcatcccggg gactcgcggt aggattactg agtggtttta atacggcata ccgcggtgca
     3901 attcatgccc cgtcagcatc tgccactatg aacaacacct ggtggcaact gacaggagac
     3961 tcctcacttc gctcgttaaa aaataccgga agcatgacat attttacagg aagtgcagcg
     4021 aataaagcat tccatacact gacggttgat gagctgacga cgaatggcac tgcgtatgcc
     4081 atgcgtacgg acctgaaaaa tgcggataag ctggtagtaa accaaaagct gtcaggtaag
     4141 gacaatattc tgctggttga ttttctgaac aaacccaccg gagaaaaact ggatattgaa
     4201 ctggtgagtg caccggggaa cagcagtaag gatgttttta aaggaagtga acaggcaata
     4261 ggtttcagca atgtcacacc tgttattaca gctaaagacg ccggagataa aacaacatgg
     4321 aacctgaccg ggtacaggat ggcagaaaat cccgccgcaa cccaaagtgc ctcaggcctt
     4381 gcatctgtgg ggtacaaatc atttttgagt gaggtcaaca acctgaataa acgtatgggt
     4441 gacctgcgtg acatcaatgg tgaagctggc gcatgggcac gtatcatgag cggaaccggc
     4501 tctgccggtg gtggtttcag tgacaaccac acacatgttc aggtcggtgt cgacaaaaaa
     4561 catgagctgg acggactgga tttgtttacc ggcttcactg tcacacacac tgacagcagt
     4621 gcctctgctg atgctttcaa aggtaaaaca aaatctgtgg gggccggact ctatgcttcc
     4681 gccatgtttg attccggtgc ctatatcgac ctgattggta agtatgttca tcatgataat
     4741 gagtacaccg caacctttgc cggactcgga atccgtgatt acagtacgca ttcatggtat
     4801 gccggtgctg aagcaggcta ccgctgtcat gtcactgagg atacctggat tgagccacag
     4861 gcagaactgg tttacggtgc tgtatccggt aaacagtttg catggaagga ccaggggatg
     4921 catctgtcta tgaaggacag ggactacaat ccgctgattg gtcgtaccgg tgtggatgtg
     4981 ggtaaatcct tctcaggtaa ggactggaaa gtgacagccc gtgccggtct gggctaccag
     5041 ttcgacctgc tggctaacgg cgaaaccgta ttgcgggacg catcaggtga aaaacgtatc
     5101 aaaggtgaaa aagacagccg tatgctgatg tccgttggcc tgaatgcaga aatcagggac
     5161 aacgtccgct ttggactgga gtttgagaaa tccgcctttg gtaagtacaa cgttgataat
     5221 gctgtcaacg ctaacttccg ttactcgttc tgatactccg tattcaaaag ggcctgcggg
     5281 ccctttatgc tcaggacctg ccgcccgggt atacatttcc ggggtatcag tcgatttgtt
     5341 tacttaaccg ccatgaccaa agccggcagc aatgtcggct ttttacgtcc atatattcca
     5401 ctcatccgga actgtgacac agaacctgaa tacaggattg atcaccggtg cccacaaatg
     5461 gcacattttc agtaagattc tgaatgttac attcccccgt catgtgtggc acaactgtca
     5521 ccgggaaaga aacggaaagc acatgaaaaa cataaaaact cacaccggac tccttatcca
     5581 caaagaacaa gcgcggcgcg ttcgtcttca tgaaactccg gccgcctggt gccacacgca
     5641 ccgggaatgc tacagcaaaa cgaccggtcg ccgctgcggc agccctgaca gtctcagcag
     5701 gctgattctg agcagcattc agcccataga atgagatgac ccgtatgaat gatggcagac
     5761 agacgcctcc gtcccctgag gcatggaaaa tccggtttct ggcgaacggt acacccggca
     5821 catggtttta caccgatgac gcagccgtca caaaagagtg gctgcgggca cattgtgacg
     5881 atgtggaagt gatccccctg tgccgtatgc atgacggtcc cgttttccgg gaacaccata
     5941 ttaatgaact cagcgccgca ctggccagaa tgctccatat gcaccacata cagctggaac
     6001 gaacccggta cagagaaaat atccatgatg cagaatgtat tcgtgaaatt cacgaagctc
     6061 ccctgcaggc tcccggcatt ctggaaaaat acaaacgcac aaaatcataa ggcagttcta
     6121 tgtggatact gattttctgg ctctcatccc cctttaatgt ctcctccggc gatgcccggc
     6181 aacccacact ggccgttcac agtcaggagt tccgtactga aaaagcctgt cgggatgcct
     6241 tcagcgcgct gaaaaaagaa aacgtgggag aattcgctct ccggggcgtc tgcgtccccg
     6301 gcggagaata aaccggagat gatgattctc cacaggagta ttcaatggca taccattccc
     6361 cggatgtcac actacattat ccgtatctgc gccggatgtc ctgggcgcag ataacggcag
     6421 cagctgaaga tgcggaaata catcatgatt attcccgggc attaatattg tggcagcatg
     6481 catatcatgc tgccacgctg actctcaaca aaaacctggc agttgctaaa atcgacttct
     6541 gtattaaacg tatccggatg catgaacaaa tgagcaggat aatccggaag actggcacag
     6601 acgaaatact cttctgaacc ggccccttga aacgccagac agtggatgta tcttaaaata
     6661 agagataggc ttggatat
//
LOCUS       DAZGAN020000185         6483 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGAN020000185 DAZGAN020000000
VERSION     DAZGAN020000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.185"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            263..478
                     /locus_tag="ACHKYO_RS23760"
                     /old_locus_tag="pgaptmp_004752"
     CDS             263..478
                     /locus_tag="ACHKYO_RS23760"
                     /old_locus_tag="pgaptmp_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4431442.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            652..909
                     /locus_tag="ACHKYO_RS23765"
                     /old_locus_tag="pgaptmp_004753"
     CDS             652..909
                     /locus_tag="ACHKYO_RS23765"
                     /old_locus_tag="pgaptmp_004753"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431443.1"
                     /translation="MERTQLINHVRGLLAEYGIVFSKGATELRQKLPALLEDAENELT
                     DTMKTLLHRQYIRLITLDNELEWYDSELKNMSAKILCANAC"
     gene            891..1082
                     /locus_tag="ACHKYO_RS23770"
                     /old_locus_tag="pgaptmp_004754"
     CDS             891..1082
                     /locus_tag="ACHKYO_RS23770"
                     /old_locus_tag="pgaptmp_004754"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4431444.1"
                     /translation="MCKRLLAIPRFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            1224..2243
                     /locus_tag="ACHKYO_RS23775"
                     /old_locus_tag="pgaptmp_004755"
     CDS             1224..2243
                     /locus_tag="ACHKYO_RS23775"
                     /old_locus_tag="pgaptmp_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HGU4431445.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWGRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PDKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMREASAFKSGREFAAYVGLVPKQTGSGGKIRLLEISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            2964..3179
                     /locus_tag="ACHKYO_RS23780"
                     /old_locus_tag="pgaptmp_004756"
                     /pseudo
     CDS             2964..3179
                     /locus_tag="ACHKYO_RS23780"
                     /old_locus_tag="pgaptmp_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            3354..5324
                     /locus_tag="ACHKYO_RS23785"
                     /old_locus_tag="pgaptmp_004757"
     CDS             3354..5324
                     /locus_tag="ACHKYO_RS23785"
                     /old_locus_tag="pgaptmp_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HGU4431446.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYSPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGFRYDR
                     YKSFNPRAGKLKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKSFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            5343..6134
                     /locus_tag="ACHKYO_RS23790"
                     /old_locus_tag="pgaptmp_004758"
     CDS             5343..6134
                     /locus_tag="ACHKYO_RS23790"
                     /old_locus_tag="pgaptmp_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HGU4431447.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAANGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         1960 a         1421 c         1537 g         1565 t
ORIGIN      
        1 aggcctcccg ccaaacgtaa aaacactcac aagggaacat agattcaatg cgtttattta
       61 gaaggtctgt cttcttttta atattaactt attaattggt gctattcata taattcttta
      121 aacagcgaac ttatagccag gattcaatag atatagtaac aggaacatca gtaagcgatg
      181 agatggtctt ccccctcctt cacgacaacc tttaattacg tcatgatgga taaactttaa
      241 ccacaaaagt aagagggata ccatgtctca acagaaaatc accggcatcg atctggctaa
      301 aacgaacttt tatcttttca gtatcaatgc tcatggcaaa cccgcaggaa ggatcaaact
      361 ttctcgcaat caactgctta actggctggt gcagcagcct aaaatgactg ttgcgatgga
      421 ggctcgcggg catcccatca ctgggccagg gaaatcagga aactcgacca tgatgtaata
      481 tttcttccgg cgcaacatgt taaagcttat cagcgacgcc agaaaaatga ttatgacgac
      541 gctcaggcaa tagctgaggc ctgccaacat ggaactatac gccccgtacc aataaaaact
      601 ctggagtaac aggacgtcca gacttttttg aaaatgcgcc ggttggtttc gatggaaagg
      661 acacaactga taaatcacgt tcgtggactt cttgctgaat atggcattgt gttcagtaaa
      721 ggcgcaacag aattacgcca aaaattaccg gcattacttg aggatgcgga aaacgaactc
      781 actgatacca tgaagacgtt attgcatcgg cagtatatac ggttgattac actagataat
      841 gaactggagt ggtatgattc tgaactgaaa aatatgtccg ccaagatcct atgtgcaaac
      901 gcttgttagc cattcctaga ttcagccctc tggttagtca ggcaataaaa agctggatgg
      961 gggatggaaa acagtttaga cgaggaagag atgcatctgc gactctggga ctggttcccc
     1021 gacagttcag cacaggtggg aagcaggttc ttctgggtat aacaaaattt ggagaaatgt
     1081 aatgaacaca tcccacccca ttaagtcagg tgctctgtga tggatgaagg gaaatactgg
     1141 aaggtgtgga gcctcgcaga tttccggcat catagtgccc aatacaggat gcataaagct
     1201 ccctcagact ggagactcca caaatgaaat atacaccggt tggcgttgat atcgcaaaac
     1261 atctgattca ggttcatttc atcgacgagc acacaggtga agtgattgat aagcaactac
     1321 gtcgtcagga ttttctgatg ttcttctgta atcgggaacc ctgcctgatt ggtatggagg
     1381 cctgtagagg ctctcagcac tgggggcgtg aactgacaaa gctggggcat aaagtccggt
     1441 tgttacaggc ccactttgtt aaggcattcg tcatgggaaa taagaatgat gtgatggatg
     1501 cccgggctat ctggatggct gttcagcagc cagataagga catcgcggtt aaaacagaag
     1561 aacaacagtc agtgctggtt ttgcaccgtt cccgcagaca attggttaag ttccgcacca
     1621 tgcagattaa ttctctgcac ggcacgctcc tagaatttgg taaaaccatt cacaaaggtc
     1681 gggcagcgat ggagcgagag ctccctgaag cactggagcg gatgaaagag agactgccgc
     1741 cgtatctcat tactgtgctg gaaaaccagt acaaccggct gaatgagctg gactcgctga
     1801 tagaggatat tgaaaaaaag cttaccagcg tggcgagaca gaatgaaacc tgtaagcggt
     1861 tgctggatat tcccggcgtt gggccactta ttgcaacggc agcggtggcc accatgaggg
     1921 aagcatcagc gtttaaatca gggcgagagt tcgcggctta tgttggtctg gtgcccaaac
     1981 agactggctc aggagggaaa atccgcctgc tggagataag caaacgtggt aatacatatc
     2041 tgcgagcctt gtttattcat agcgcaaggg cggcagcatt actgcccaaa gaacctggtc
     2101 cgtggataac cgaactgaaa aaacgtcgtc caaccagcgt ggcaatcgtc gccatggcaa
     2161 acaaactagc acgaacagta tgggcgatag cagctcatgg aagtaaatac gacaagaatc
     2221 atgtcagtat cagatcatat taatcgctga taccgataaa tattaacttt taatgaaagg
     2281 atgaatactg aaaggtaact ctggcggcca tagtgatgac aacgacaggt aagaccggga
     2341 ctcactaaac ctgaatattg tgttgagcta aaagctcgct ggagaaatga ggtgtgagtc
     2401 agcgaattac ataggggctc gcagcttcat ggctgcaaaa aagccggata taaagctgca
     2461 acctacccgt cactgtcaag gcaatggatg ccttgcaacg ggatgtgttc atataatcaa
     2521 tatgtcagat ctatgctgat acagggtgcc agagcggtat tgttcagagc ctcagagaaa
     2581 actgattcac ttcgtgtgtg ggttaattgt attcgggaaa agcgtggctt caaccaggca
     2641 gttgttgctc ttgcaaataa actccttcgg atcgcctggg caattatcgc ccgcaatgag
     2701 atgtatttgc ccgcagcaaa acaaaatcaa taatctacgt aaaatcagta ggagtaaagc
     2761 ttaccctgaa attgcaaaga atgcagaatg atgaaaaaca ggtcagaccg acgttacgaa
     2821 aacctgcaag atgagccagg ccagtaaggc cgagtaagcg ataaggtcgg agcgcgcgaa
     2881 tgtctcatca aggccagaag caataacttc atgaacaaac cggatatacg aaagtaaacc
     2941 tgtccctatc atagttgctg tactcttgca atacggggga agaccatctc atcgcttacg
     3001 aacaattcgt gaaaacgatg aagaaagact acatcgcgtg catgccgaaa tctaatgtaa
     3061 gaacggcatt gcataatctt gcagtggaga tcgggtatta caatgaaaac catccgcata
     3121 gtgcgttagg ttatctccca ccaagggaat atcaacgtca gcgaataacg tcaccttaga
     3181 tgcagtagtt gtcaggaaat agtgactcaa gggcaggctt accatataca ctcttagtgg
     3241 ttcgactggg tattagaata gctattaaca gattagcact gaacactgcg taatagtggt
     3301 tgcacaaaca aaatgttatg ttataacatt gctgtattga catatatttt cgaatgtata
     3361 tgaacgtaat cagaactgtc atttgtacat taatcatact tccggtgggg ttacaggcag
     3421 cgacaagtca ttcttctatg gctaaagata caatcaccgt tgtcgcgaca ggaaatcaga
     3481 acacggtatt tgaaacgccg tcgatggtca gtgtcatcac gaatgacacg ccatggagtc
     3541 agaatgcggt cacatcagcc ggtatgctga aaggtgttgc gggtctcagc cagactggtg
     3601 caggacggac caatgggcag acctttaatc tacgcggcta tgacaaaagc ggggtacttg
     3661 ttcttgttga cggcgtccgt caactcagtg acatggcaaa aagcagtggc acttatctgg
     3721 atccggcact catcaaacgc atcgaagttg tccgcggacc aaactccagt ctgtacggca
     3781 gtggcgggct ggggggggtg gtggacttca gaactgccga tgctgcagat tttcttcccc
     3841 ccggagagac aaatggtgta agtctgtggg gaaatatcgc cagtggtgac cacagcacag
     3901 gctcggggct cacctggttc ggtaaaaccg gaaaaacaga tgcgcttctt tctgtcatta
     3961 tgcgtaaaag aggaaatatc tatcagagtg atggtgagcg tgcacctaac aaggaaaaac
     4021 ctgcagccct gtttgcgaaa ggctctgtca gtataacaga cagtaacaaa gcaggtgcca
     4081 gcttgcgtct ctaccagaat aacaccactg aaccgggcaa tcccactctg acacatggtg
     4141 acaacggcct gcgtgacaga aaaacagcac aaaatgacgt gcagttctgg taccagtact
     4201 cccctgcgga taacagcctc atcaatgtaa agtcaacgtt atatctcagt gatatcacta
     4261 tcaagacaaa cggtcacaac aaaacagcag aatggcgaaa caacagaacc tccggtgtta
     4321 atgttgtcaa caggagccat actctgattt ttccgggagc ccatcagtta agttatggcg
     4381 ccgaatatta ccgtcagcag cagaagccgg aaggctctgc cacactatat ccggaaggaa
     4441 acattgactt tacatcgttg tatttccagg atgaaatgac aatgaaaagc aacccggtta
     4501 acattatcgt cggtttccgt tatgaccggt acaagagctt caatccccga gccggaaaac
     4561 tgaaagccga acgcctgtcc cccagggcgg cgatttcagt ctcaccaaca gactggctga
     4621 tgatgtacgg ctccatatcc tctgcattcc gtgcccccac aatggcagaa atgtacaggg
     4681 atgatgtaca tttttaccgc aagggtaaac ccaattactg ggtacctaac cttaatctga
     4741 aaccagaaaa taacattacc cgtgagattg gcgcaggtat tcaactggac ggcctgctta
     4801 cagacaatga ccggctgcag ttgaaaggcg gatatttcgg aacggatgcc agaaactata
     4861 ttgccacgcg cgtggatatg aaacggatgc gttcttattc ttataatgta tcccgggccc
     4921 gtatctgggg atgggatatg cagggtaatt accagtctga ttattttgac tggatgcttt
     4981 cttataaccg cacggaaagt atggatgcca gtagcaggga atggctgggc tccggcaatc
     5041 ctgacacact catcagtgac atcagtatac ctgttggtca tggaggagtt tctgccggat
     5101 ggcgtgctga actttcggca gcagccacgc atgtgaaaaa aggcgatccc catcaggctg
     5161 gctatgccgt acattccttt tcactgtctt ataagcctgt aagtgttaaa agctttgagg
     5221 catcagtgac tctggataat gccttcaaca agcttgccat gaatggcaaa ggtgtgccgc
     5281 tttcaggcag aactgtcagt ctttataccc gttatcagtg gtaacctcgc aggaaataaa
     5341 tcatgatgaa aaatacaggc tatatcttag ctctttgtct gacagcatcg gagcatgccc
     5401 tggcccacga tgtctggatt accggtaaac aggcagagaa caacgttacc gcagagattg
     5461 gttatggtca taatttcccc tcaaagggca caattcctga cagaaggaat ttctttgaaa
     5521 atccccggct ttataacggg aaagagataa taacgctgaa gccaacgtcc acggattatg
     5581 tctataaaac tgagtctgca gccaacggta atagctacgt tctgtcaacg aaaatgaaac
     5641 cgggatactg gtcgagaacc tcgtcaggat ggaagcctgt cagtcgggag ggcagaaatg
     5701 atgtggcata ctgtgagttt gtgactaaat atgcaaaatc ttttattcct ggtgaacagc
     5761 agatgccagc acaactctat cagtctccaa caggggatga gcttgaaatc attccattat
     5821 ccgatatcag tcgtttcggt gaagatgtga agctgaaagt tctgtataaa acatccccgc
     5881 ttgccggagc tactctggag cttgactctg tcagttatct gaaatcatcc cgtcataccc
     5941 atgcagctga gcataaacat tcggctcata aagcagaact tacctttgta agtaatgagg
     6001 acggtatcat cactgtacct tcccttcatg tcgggcaatg gctggctaaa gtcaaaaata
     6061 agaaagtatt tccggacaag agtctgtgtg atgaaactgt cgatgtggca accttaagct
     6121 tctcccgaaa ttaataatac tattcctgcc ctgatttatg ggcagggtat tatcaatacg
     6181 agaatagcct accgacatcc gtgtaaaaaa ccacataatg atttttcata tccatttcca
     6241 gatatactta tgtaaagatc acctccaaaa aagcgtaata gtaataaaaa tccctgaagc
     6301 accctgttca aaccagaagg aatacataac aagacaataa aatgtgaaat ccatcacagc
     6361 aaagaactct ttgtttataa tataatgaaa attagctaat attctttaac aagaactgtt
     6421 ggtcatataa aattaaatat ttttatgcta aatggttcca tacgtgaagt tatatcagaa
     6481 taa
//
LOCUS       DAZGAN020000186         6297 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGAN020000186 DAZGAN020000000
VERSION     DAZGAN020000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.186"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1331)
                     /locus_tag="ACHKYO_RS23795"
                     /old_locus_tag="pgaptmp_004759"
     CDS             complement(<1..1331)
                     /locus_tag="ACHKYO_RS23795"
                     /old_locus_tag="pgaptmp_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4431448.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHNKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKYSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDDLNAVNNYLLNNKKATDN
                     EINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKFPYINDVRILDWCFNKRMQYF
                     GDSEKIKYACSVINHIDFSRDQSKDFSCDQSKIKIAETLFFNLDKEHYKNSRKLQELI
                     WDKLVAYVNDFNLSNQEKSRLILRLFDDVKLLFNEVPVSILVNDIFFEGFF"
     gene            complement(1453..1905)
                     /gene="tabA"
                     /locus_tag="ACHKYO_RS23800"
                     /old_locus_tag="pgaptmp_004760"
     CDS             complement(1453..1905)
                     /gene="tabA"
                     /locus_tag="ACHKYO_RS23800"
                     /old_locus_tag="pgaptmp_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HGU4431449.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTVETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(2050..2643)
                     /locus_tag="ACHKYO_RS23805"
                     /old_locus_tag="pgaptmp_004761"
     CDS             complement(2050..2643)
                     /locus_tag="ACHKYO_RS23805"
                     /old_locus_tag="pgaptmp_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU4431450.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILLDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            2714..3427
                     /gene="bdcA"
                     /locus_tag="ACHKYO_RS23810"
                     /old_locus_tag="pgaptmp_004762"
     CDS             2714..3427
                     /gene="bdcA"
                     /locus_tag="ACHKYO_RS23810"
                     /old_locus_tag="pgaptmp_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4431451.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3558..3953
                     /locus_tag="ACHKYO_RS23815"
                     /old_locus_tag="pgaptmp_004763"
     CDS             3558..3953
                     /locus_tag="ACHKYO_RS23815"
                     /old_locus_tag="pgaptmp_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU4431452.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            4234..4368
                     /gene="pyrL"
                     /locus_tag="ACHKYO_RS23820"
                     /old_locus_tag="pgaptmp_004764"
     CDS             4234..4368
                     /gene="pyrL"
                     /locus_tag="ACHKYO_RS23820"
                     /old_locus_tag="pgaptmp_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HGU4431453.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            4372..5307
                     /gene="pyrB"
                     /locus_tag="ACHKYO_RS23825"
                     /old_locus_tag="pgaptmp_004765"
     CDS             4372..5307
                     /gene="pyrB"
                     /locus_tag="ACHKYO_RS23825"
                     /old_locus_tag="pgaptmp_004765"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HGU4431454.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWRLHSSIEE
                     VIAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            5320..5781
                     /gene="pyrI"
                     /locus_tag="ACHKYO_RS23830"
                     /old_locus_tag="pgaptmp_004766"
     CDS             5320..5781
                     /gene="pyrI"
                     /locus_tag="ACHKYO_RS23830"
                     /old_locus_tag="pgaptmp_004766"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HGU4431455.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5854..6240
                     /gene="ridA"
                     /locus_tag="ACHKYO_RS23835"
                     /old_locus_tag="pgaptmp_004767"
     CDS             5854..6240
                     /gene="ridA"
                     /locus_tag="ACHKYO_RS23835"
                     /old_locus_tag="pgaptmp_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HGU4431456.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
BASE COUNT         1715 a         1506 c         1388 g         1688 t
ORIGIN      
        1 tgaaaaaatc cttcaaaaaa aatatcattt actaaaatac tgacaggcac ttcattaaat
       61 agcaacttaa catcatcaaa taaccttagt attaagcgtg atttttcttg attgcttaag
      121 ttaaaatcat tgacatatgc aaccaactta tcccaaatca attcctgtaa tttacggcta
      181 tttttatagt gttctttatc gagattgaaa aataaggtct cagctatttt aattttagat
      241 tgatcgcagc taaaatcttt agattgatcg cggctaaaat caatatgatt tatcacggag
      301 catgcatact ttatcttttc agaatcacca aaatattgca ttcttttatt aaaacaccaa
      361 tccagtattc ttacatcatt aatatatggg aattttaaaa aagtctccag aatcgaatcg
      421 atgaccggtg taaggttaac ttttgcatca tctaaggaat caataagaat atgagacagg
      481 ttaactttga tgtcattaat ttcattgtca gtagctttct tattattaag aagataatta
      541 tttactgcgt taaggtcatc tatttctttc agtatccctt tttcaccaag caagtatgaa
      601 atcgattcat tacatacata aggcattaat agcagatgtt ctttccttat tgctaataat
      661 gcattattca atggtttttc taataaatcc gtaatcgtag aatcaactga atatttacaa
      721 ctcacctgcg agttattcaa gtcctgagaa ttatttaact cctgcggaat atttaataga
      781 cgagctattt ccttgttagc ttcgcgttgg taacacgcca gaatcttgtt agtaatcagg
      841 tgtaatgaac tctcatcttt tctttcaaag gacttccctt cataacgatt agtcgaacca
      901 tcaggttgtg taaatataat gactgtatgt ccttcacgaa tcccattatt aaaagctgag
      961 gcaagtgtaa tttctatttc caccttagat aaatccgctc gctccccttc aggattattc
     1021 ataacaaact tcattatctc actaccatca ttatttttga tataagatga ggacttatta
     1081 tgagccatca tatttctacc cgcttcgttg gagcgtttgc taaaatccac ataacccagg
     1141 gtaaatatct tagccaaaaa ctccaaaaat gctctgggag aaagaatatt gcttctgcta
     1201 ttctgccctc cggcaattcg ggcaaaatca ttcattccca cataccaggt atctaagtct
     1261 tcattttttt gtttacaatt gtcggctgat gtaatgtttt gagaaaaatt ccaacctgat
     1321 attttgctca tgacggactc cttgagttaa taagaacgtc taagcattac aaattttact
     1381 cacaaaaaat atcaaaaaac gctcttttca caaactcgcc gtaaacctgg agttatttac
     1441 ggcgaaaagt catcacgcca tcagcatctt cactactgct ttgcgaacct gggctggtgc
     1501 acccactgcg cacagcggtt tatgcacttc ccccggataa aacacaacaa aatcaccttc
     1561 attaaggata actgttttct catcaacgcc ttccggcaaa aatgcgatgt ctttatcagc
     1621 taaccagtcg gtatccggcg tacctgcagg ttgtgtgctg aaggtcatgc cttcctgacc
     1681 ttttaacaca atctgaatgt cgagatagcg ggcatggtac tccgcacggc gagcttcgta
     1741 cggctcggtc atatcttccg agataagata aaacagacga ttgccttcga tatcgtgctt
     1801 gccctttggc gtttctaccg taacgtgtgc tttgatatgc tcaatcgcct ggcgtaactc
     1861 ctggggtagc cacggctgaa gattatgaat atttccgatg atcatctttt ccactcctta
     1921 attaaaacaa cgtttcattt ctatcgttat acgaaaagat gatcaattcc gctagcctta
     1981 acgcgtgaat tattgtctta tcacaactta tcggcaggcg cgttcccgcc tttcgggtca
     2041 aggcccgcat cactccttga gaatagtttt tatcgcttcc cccgccagtg cagctgttgc
     2101 acagagttgt tcgatggagt gcccctcccg tgccttcgca gaaagccctg acatcacggt
     2161 actcataaaa tcagtcacac attgtgcgct ttgtggatgc cgcctggcga tatagtcata
     2221 aatggtcgtt tctgcggcgt gataatattg aacggcaata tcacgcgctt gtggatcatg
     2281 actatgaata ccttcaagaa ccatacagcc agcgcagccg ccgttttggc tatatctgcg
     2341 cgccgcttct tttaatacct caaccaggca ctcgcctacc ggacgatcat caagaagaat
     2401 atcggcaagc ggaatagctt ccgtaccgac atattcattg agtacacggc taaataaccc
     2461 agccttactg ccaaaagccg cgtagaggct cggggggtta ataccaagat aatcagtaac
     2521 ttcagcaaca ctgacagcat cgaaaccttt ttggtgaaaa agcacttttg ccgcagagac
     2581 tgcctgctca ggagcgaaac gtctgggacg acctggaacg cgagattgtt ttttagtgac
     2641 cattacaaaa cttgttgaca gaaagttaaa acagttttgt aatgcatgtt acataataaa
     2701 tcaaggagtc cttatgggcg cttttacagg taagacagtt ctcatcctcg gtggtagccg
     2761 tggaatcggt gccgctatcg tacgtcgttt cgtcaccgat ggggccaatg tacgattcac
     2821 ctatgcgggg tcgaaagatg ccgctgaacg cctggcacaa gagacgggag cgacagcagt
     2881 attcacagat agtgctgaca gagacgctgt tattgatgtc gttcgtaaga gcggcgcatt
     2941 ggatatcctc gtggtaaatg caggtattgg cgtctttggc gatgccctgg aattaaatgc
     3001 cgacgatatt gatcgccttt tcaaaatcaa tattcatgct ccttaccatg cctccgttga
     3061 agccgcccgg cagatgcccg aaggcgggcg catcttaatc atcggctccg tgaatggcga
     3121 tcgtatgcct gttgcaggca tggctgctta tgccgccagc aaatctgccc tgcaaggcat
     3181 ggcgcgcggg ctggcccgtg attttggacc gcgtgggatc accatcaacg tcgtccagcc
     3241 agggccaatt gataccgacg ctaatcccgc caacgggcca atgcgcgata tgttgcatgg
     3301 ttttatggct atcaaaagac atgggcaacc ggaagaggtc gctggtatgg tcgcatggtt
     3361 agcagggcca gaagccagct ttgttaccgg cgcgatgcat accattgatg gcgcgtttgg
     3421 cgcataaccg actacgctca attaagccca gccatttccc atgatgtctg ggttttgttt
     3481 actcacgtcg tccgctaaaa gcggctcctg gtaaatataa atcttctgtt catgtcgtaa
     3541 atcttgagga taaccaaatg gtagaaagaa ccgctgtttt ccctgctggc cgacattcac
     3601 tgtatgctga acatcgttat tctgcggcta ttcgctccgg cgatttgctg tttgtttccg
     3661 ggcaagtagg aagtcgagag gacggaacac cagaacccga ttttcagcaa caagtcagac
     3721 tggcatttga taatttgcat gcgaccctgg ctgctgcggg atgcactttt gacgatatca
     3781 ttgatgttac gagcttccat accgatccag aaaaccaatt tgaagacatc atgacggtga
     3841 aaaatgaaat atttagcgcc ccaccttatc caaactggac ggcggtaggt gttacatggc
     3901 tggcaggctt tgattttgaa attaaagtga tagcgcgcat ccctgagcag taagcaatag
     3961 tgttagccgt tcgttttcac actccgccct ataagtcgga tgaatggaat aaaatgcata
     4021 tctgattgcg tgaaagtgaa aaagaaaaaa gcaggaaatg tctgcaatta ttgataccga
     4081 acgaccgttc ccctgcagaa tcacaacaaa taaaaatgca tgtattttga cttataattc
     4141 aaataaaccg tttgcgctga caaaatattg catcaaatgc ttgcaccgct tctgacgatg
     4201 agtataatgc cggacaattt gccgggagga tgtatggttc agtgtgttcg acattttgtc
     4261 ttaccgcgtc tgaaaaaaga cgctggcctg ccgtttttct tcccgttgat cacccattcc
     4321 cagcccctca atcgaggggc ttttttttgc ccaggcgtca ggagataaaa gatggctaat
     4381 ccgctatatc agaaacatat catttccata aacgacctta gtcgcgatga ccttaatctg
     4441 gtgctggcga cagcggcgaa actgaaagca aacccgcaac cagagctgtt gaagcacaaa
     4501 gtcattgcca gctgtttctt cgaagcctct acccgtaccc gcctctcttt cgaaacatct
     4561 atgcaccgcc tgggtgccag cgtggtgggc ttctccgaca gcgccaatac atcactgggt
     4621 aaaaagggcg aaacgctggc cgataccatt tcggttatca gcacttacgt cgatgcgata
     4681 gtgatgcgtc atccgcagga aggtgcggcg cgcctggcca ccgagttttc cggcaatgta
     4741 ccggtactga atgccggtga tggctccaac caacatccga cgcaaacctt gctggactta
     4801 ttcactattc aggaaaccca ggggcgtctg gacaatctcc acgtcgcaat ggttggtgac
     4861 ctgaaatatg gccgcaccgt tcactccctg actcaggcgt tagcgaagtt cgacggcaac
     4921 cgtttttact tcatcgcgcc ggacgcgctg gcaatgccgc aatacattct ggatatgctc
     4981 gatgaaaaag ggatcgcatg gaggctgcac agctctattg aagaagtgat agcggaagta
     5041 gacatcctgt acatgacccg cgtgcaaaaa gagcgtctgg acccgtccga gtacgccaac
     5101 gtgaaagcgc agtttgttct tcgcgccagc gatctccaca acgccaaagc caatatgaaa
     5161 gtgctgcatc cgctgccgcg tgttgatgag attgcgacgg atgttgataa aacgccacac
     5221 gcctggtact tccagcaggc aggcaacggg attttcgctc gccaggcgtt actggcactg
     5281 gttctgaatc gcgatctggt actgtaaggg gaaatagaga tgacacacga taataaattg
     5341 caggttgaag ctattaaacg cggcacggta attgaccata tccccgccca gatcggtttt
     5401 aagctgttga gtctgttcaa gctgaccgaa acggatcagc gcatcaccat cggtctgaac
     5461 ttgccttctg gcgagatggg ccgcaaagat ctgatcaaaa tcgaaaatac ctttttgagt
     5521 gaagatcagg tagatcaact ggcattgtat gcgccgcaag ccacggttaa ccgtatcgac
     5581 aactatgaag tggtgggtaa atcgcgcccc agtctgccag agcgcatcga caatgtgctg
     5641 gtctgcccga acagcaactg tatcagccat gccgaaccgg tttcttccag ctttgccgtg
     5701 cgaaaacgcg ccaatgacat cgcgctcaaa tgcaaatact gtgaaaaaga gttttcccat
     5761 aatgtggtgc tggccaatta attgcggttg gtaataaaag tctggctccc tataatgagc
     5821 cagacttttt accgctgtaa taaaggagaa atcatgagca aaactatcgc gacggaaaat
     5881 gcaccggcag ctatcggtcc ttacgtacag ggcgttgatc tgggcaatat gatcatcacc
     5941 tccggccaga tcccggtaaa tccgaaaacg ggcgaagtac cggcagacgt cgctgcacag
     6001 gcacgtcagt cgctggataa cgtaaaagcg atcgtcgaag ccgcaggcct gaaagtgggc
     6061 gacatcgtta aaaccaccgt gtttgtgaaa gatctgaacg acttcgcaac cgttaatgct
     6121 acctacgaag ccttcttcac cgaacacaat gccaccttcc cggcacgttc ttgcgttgaa
     6181 gttgcccgtc tgccgaaaga cgtgaagatt gagatcgaag cgatcgctgt tcgtcgctaa
     6241 tcttgatgga aatccgggct atcatgcccg gattaagtct gatgacaaac gcaaaat
//
LOCUS       DAZGAN020000187         6285 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGAN020000187 DAZGAN020000000
VERSION     DAZGAN020000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.187"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            362..880
                     /locus_tag="ACHKYO_RS23840"
                     /old_locus_tag="pgaptmp_004768"
     CDS             362..880
                     /locus_tag="ACHKYO_RS23840"
                     /old_locus_tag="pgaptmp_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGU4431457.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1090..3231
                     /locus_tag="ACHKYO_RS23845"
                     /old_locus_tag="pgaptmp_004769"
     CDS             1090..3231
                     /locus_tag="ACHKYO_RS23845"
                     /old_locus_tag="pgaptmp_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU4431458.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDHSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HHENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARPVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3307..>6285
                     /locus_tag="ACHKYO_RS23850"
                     /old_locus_tag="pgaptmp_004770"
     CDS             3307..>6285
                     /locus_tag="ACHKYO_RS23850"
                     /old_locus_tag="pgaptmp_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HGU4431459.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQYSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAKGDVAGAVTGVTDGAGRRFHLALTTQAQRAEAFRKQRASSLSSPASPRSV
                     SSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGELRAV
                     YDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYRLHMASGKLTSVILPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTSYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQMVSQKDAQGRETRYEYSAAGDLTATVSPDGKRSTI
                     AYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTRKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVTPDSL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSRAGSNAAYELTSTYTPA
                     GQLQSQHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIR
                     IPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDRIPTGV
                     IRTDDERTHHYHYDSQHRLVF"
BASE COUNT         1475 a         1660 c         1969 g         1181 t
ORIGIN      
        1 tgttttgtga ttgatcaagt gggtattttt aaggaatatc tcaaaaatta tcaaaatgat
       61 aattcagaaa ggataaatac aatatttatt catgattcag ctttggccat tttatggagg
      121 caatacggac tttgaacagt tgggtatgat tatccagata tatattatta agttattgct
      181 gaataagaga tttaatgatt ttaaaaatag tttttacgac aaccgtaaat tctagctcgg
      241 tttgtatgta aaaaatactg aaaaatgatg cggttccgtg aagtgagctg catctgcctg
      301 tctgggatta acggcaggtc attgtaagta gtaattaacc acgttaacaa ggagtttaca
      361 aatgccaaca ccgtgttata tctccatcac tggtcagacc cagggaaata ttactgcagg
      421 tgcatttacc gctgattctg tcggcaacat ctatgtgcag gggcacgaag acgagatgct
      481 ggtgcaggaa ttcctgcata acgttactgt cccgaccgat cctcagtccg gtcagcctgc
      541 gggtcagcgt gctcacaagc ctttcatttt taccgtggcg ctgaacaaag cggttccgct
      601 gatgtacaac gcactggcct ccggcgaaat gctgccaacc actgaacttc actggtggcg
      661 tacctctgtg gaaggcaaac aggagcatta tttcactacc cgcctgactg attcgacgat
      721 tgtcgatatg aaactgcata tgccgcattg ccaggacccg gcgaaacgcg agttcaccca
      781 gttgctcgaa gtttcactgg cctaccgcaa aattgaatgg gaacacgtca aatctggcac
      841 ctccggtgcc gacgactggc gcgcaccgct ggaagcataa gttaagccaa cagcatccgg
      901 ctatcaggcc ggatgctgtt tttgtattac gccgtagaaa gaaaggttgt cagtaagggg
      961 ttacgttcac gggagatggc ggctgccgga aacttcggcg gtagcgtgcc tgaacgcaag
     1021 cgtaacactg tactgtcaca gccgctttaa cagcgaactg tctgtttttc cttaaccccg
     1081 gaggttgcta tgtcaaccgg attacgtttc acgctggaag tggacggcct gccaccggat
     1141 gcttttgcgg tggtctcctt tcatctgaac cagtcactct cttcgctttt ttccctcgat
     1201 ctctctctgg tcagccagca gtttctctcc cttgaattcc agcagatcct cgacaaaatg
     1261 gcctacctga cgatatggca gggcgatgac gtacagcgcc gggtgaaagg tatggtgacc
     1321 tggtttgaac tgggggagaa cgacaaaaac cagatgctgt acagcatgaa ggtgtgccca
     1381 ccgctgtggc gcacagggct gcgccagaac ttccgtatct tccagaatga ggacatcgaa
     1441 agcatcctcg gcacgatatt gcaggaaaac ggggtgaccg agtggagccc gctgttcagc
     1501 gagccacatc cttcccgtga gttttgtgtc cagtatggtg agactgatta cgatttcctg
     1561 tgccggatgg cggcggagga aggcatcttc ttttatgagg agcacgcgca aaaaagtacc
     1621 gaccacagcc tggtcctgtg cgataccgtg cgttatctgc cggagtcctt tgagatcccc
     1681 tggaatccga acacccgtac cgaggtgagc accctctgca tcagccagtt tcgctacagc
     1741 gcacaaatcc gcccttcttc cgtggtgacc aaagactaca cctttaaacg acccggctgg
     1801 gcagggcgtt ttgatcagga aggccagtac caggattacc agcgcacaca gtatgaagtg
     1861 tatgactacc ccggacgttt caagggtgcc cacgggcaga actttgcccg ctggcagatg
     1921 gatggctggc gcaacaacgc agaagtggcg cgcggaacaa gccgttcgcc ggagatatgg
     1981 ccgggacggc gaattgtgct gacggggcat ccgcaggcga acctgaaccg ggaatggcag
     2041 gtggtggcga gcgatcttca cggcgaacag ccgcaggcgg taccgggacg caggggttca
     2101 ggcaccacgc tggataacca ctttgcggta atcccggcag accggacatg gcggccacag
     2161 ccgttgctga aaccgctggt ggacggcccg cagagcgccg tcgtgacggg accggcaggc
     2221 gaggaaatct tctgtgatga acatggccgc gtgcgggtga aatttaactg ggaccgctat
     2281 aacccgtcaa accaggaaag ttcatgctgg atccgtgtgg cacaggcgtg ggcaggcacc
     2341 ggctttggta acctggcgat accgcgtgtg ggtcaggagg tcattgtgga cttcctcaac
     2401 ggcgacccgg accagccgat tattatgggg cgtacctacc accatgaaaa ccgcacaccc
     2461 ggcagcctgc cggggacgaa gacgcagatg acgatccgct cgaaaaccta taagggcagc
     2521 gggtttaatg aactgaagtt tgacgatgcg acagggaaag aacaggtcta catccacgcg
     2581 cagaagaaca tgaacaccga ggtgctgaat aaccgcacca ctgatgtgat aaacaaccat
     2641 gccgaaaaaa taggtaacaa ccaggcgatc accgttacca ataaccagat ccagaacatt
     2701 ggcgttaatc agatacagac ggttggtgtc aaccaggtgg aaacggtggg cagtaaccag
     2761 attatcaaag tgggatcaaa ccaggttgaa aaggtgggga tcattcgtgc gctgacggtg
     2821 ggtgtggctt accagacgac ggtaggcggc attatgaata cctcggtggc gttgttgcag
     2881 tcctcacagg tagggctgca taaatcactg atggtgggaa tgggctacag cgtcaatgtg
     2941 gggaataacg tcaccttctc ggtgggcaag acaatgaagg aaaacaccgg acaaacagca
     3001 gtttattctg ccggtgagca tcttgaactc tgctgtggta aggcaaggcc ggtgctgacg
     3061 aaggacggaa gcatatttct caatggtacg cacattcatc tggaagggga gtcggatgtg
     3121 aacggtgatg cgccagtgat taactggaac tgtggtgcca cacaacctgt accggatgcg
     3181 cctgtgccga aagatttacc ccccggaatg ccggatatgc ggcaattttg agtcaggtgc
     3241 aaaaaattaa aacggaactg gtgcagtgac cagataaacc atattaaaag cagaaggaat
     3301 tgattcatgg gcggaaaacc ggcggcgcgg cagggtgata tgacccgcaa gggactggat
     3361 attgtgcagg gttcagcggg ggtgctgata ggtgcgccga cgggagtggc ctgttcggta
     3421 tgtccgggag ggattaccta tgctaacccg gtgaacccgc tgctgggtgc gaaggtgctg
     3481 ccgggcgaga cggaccttgc gctgcccggc ccgctgccgt ttattctttc ccgcgcctac
     3541 agcagctacc ggaccagaac acccgcgccg gtgggggtgt ttggtcccgg ctggaaagcg
     3601 cccttcgata tccgcttaca gatacgcgat gaaggcctga tactcaacga cagcggcggc
     3661 aggagcattc actttgagcc gctgtttccc ggcgagataa gctacagccg cagcgagtca
     3721 ctctggctgg cgcggggcgg agtagcggcg cagtacagtt cgcagccgct aagcgcgctc
     3781 tggcaggtgc tgccggaaga tgttcgcctg agtccgcatg tgtacctggc gacaaacagc
     3841 cttcaggggc cgtggtggat actcagctgg ccggagcggg tgccgggggc ggacgaggtg
     3901 ctgccgccgg agccgcccgc ataccgggtg ctgacgggcg tggtggacgg cttcgggcgg
     3961 acgctggcct ttcaccgggc agctaagggt gacgtggcgg gcgcggtgac gggggtgacg
     4021 gacggcgcgg gccgccgttt tcacctggcg ctgaccacgc aggcgcagcg ggcggaagcc
     4081 ttccgtaaac agcgcgcctc ttccttatct tccccggcca gtccccgttc tgtttcctct
     4141 tctcaggttt tccccgacac gctgcccgcc ggtacagagt acggtgccga taacggtatc
     4201 cggctggagg cggtatggct gacacacgac ccggcatacc cggatgaaca gccgactgcg
     4261 ccgctggcgc gctacacgta cacggccagc ggagaactgc gggcggtgta tgaccgcagc
     4321 ggaacgcagg tgcgcgggtt tacttatgat gcggagcacg ccgggcggat ggtggcgcac
     4381 cattatgcag ggagaccgga gagccgctac cggtatgatg ataccgggcg ggtgacggag
     4441 caggtcaacc cggaggggct ggactatcgc tttgagtacg ggcaggaccg tgtgaccatc
     4501 acggacagcc tgaaccggcg ggaggtgctg tacacggagg gcgaaggcgg cctgaagcgt
     4561 gtggtgaaga aggagcatgc ggacggcagc atcacccgca gcgagtatga tgaggcgggg
     4621 aggctgaagg cgcagacgga tgcggcgggg cggcggacgg agtaccggct gcatatggcg
     4681 tctggaaagc tgacttcagt gatactgccg gatggcagga cggtacggta tggctataac
     4741 agccagcggc aggtgacgtc ggtgacgtac ccggacgggc ttcgcagcag ccgggagtat
     4801 gatgagaagg gaaggctggc ggcggagacc tcacgcagcg gtgagacgac gagttacagc
     4861 tatgatgacc cggcgagtga gctgccgaca gggatacagg acgcgacggg cagtacaaaa
     4921 cagatggcat ggagccgtta cggtcaactg ctggccttta cggactgctc ggggtacacg
     4981 acgcggtatg agtacgaccg gtacggtcaa caaatcgccg ttcaccggga agaaggcatc
     5041 agcacttaca gcagttataa cccgcgtggg caaatggtca gtcagaagga tgcgcagggg
     5101 cgtgaaaccc gttatgagta cagcgccgca ggagacctga cagcaacggt atcaccggac
     5161 ggtaaacgca gcaccatcgc atatgataaa cgcgggcgtc cggtgagtgt gacggaaggc
     5221 ggtctgacgc gcagcatggg gtatgacgct gccgggcgca tcaccgtgct gaccaacgag
     5281 aacggcagcc agtccacgtt ccggtatgac ccggttgaca ggctgactga acagcgcggt
     5341 tttgacggcc ggacgcaacg ttaccactat gacctgacca gaaaactcac acagagtgaa
     5401 gacgaggggc ttatcaccct ctggcactac gatgcgtcgg accgcatcac gcaccggacg
     5461 gtgaacggcg acccggcaga gcagtggcag tatgatgagc acgggtggtt aaccaccctc
     5521 agccatacca gtgaaggcca ccgcgtgtcg gtccactacg gctacgacga taagggccgc
     5581 ctgacgggcg aacggcagac ggtggagaac ccggagacgg gggaactgct gtggcagcat
     5641 gagacgaaac acgcctacaa cgaacagggg ctggcaaacc gcgtcacgcc ggacagcctg
     5701 ccgccggtgg agtggctgac ctacggcagc ggttacctgg cgggcatgaa gctgggcggg
     5761 acgccgctgg tcgagtatac gcgggacagg ctgcaccgtg agacggtgcg cagcttcggc
     5821 agcagggcag gcagtaatgc cgcatatgaa ctgaccagca catacacccc cgcaggccag
     5881 ttacagagcc agcacctgaa cagcctggtg tatgaccgtg actacgggtg gaatgacaac
     5941 ggcgacctgg tgcgcatcag cggtccgcga cagacgcggg aatacggcta cagtgccacg
     6001 ggcaggctgg agagcgtgcg caccctcgca ccggacctgg acatccgcat cccgtatgcc
     6061 accgacccgg cgggaaaccg cctgccggac ccggagctac acccggacag cacgctcacg
     6121 gcgtggccgg ataaccgcat cgcagaggat gcgcactatg tttaccacta tgatgaatac
     6181 ggcagactga cggagaagac ggaccgcatc ccgacgggtg tgatacggac ggacgacgag
     6241 cggacccacc actaccacta cgacagccag caccgcctgg tgttc
//
LOCUS       DAZGAN020000188         6271 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGAN020000188 DAZGAN020000000
VERSION     DAZGAN020000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.188"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..524
                     /locus_tag="ACHKYO_RS23855"
                     /old_locus_tag="pgaptmp_004771"
     CDS             <1..524
                     /locus_tag="ACHKYO_RS23855"
                     /old_locus_tag="pgaptmp_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4431460.1"
                     /translation="WDAIINNQANIAIGAPDTLLDGGGIDYTEIGAIRWAFAIAPDHP
                     LAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLHGQESILVPDFNTKCQCQILGE
                     GIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLATQHSATGQVTQWIKKQFAPNGI
                     LTGIYQDLLHREN"
     gene            complement(566..1654)
                     /locus_tag="ACHKYO_RS23860"
                     /old_locus_tag="pgaptmp_004772"
     CDS             complement(566..1654)
                     /locus_tag="ACHKYO_RS23860"
                     /old_locus_tag="pgaptmp_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4431461.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKLGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(1796..5779)
                     /gene="ehaA"
                     /locus_tag="ACHKYO_RS23865"
                     /old_locus_tag="pgaptmp_004773"
     CDS             complement(1796..5779)
                     /gene="ehaA"
                     /locus_tag="ACHKYO_RS23865"
                     /old_locus_tag="pgaptmp_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HGU4431462.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKAIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         1578 a         1659 c         1313 g         1721 t
ORIGIN      
        1 tctgggatgc gattattaat aatcaggcca atatcgccat cggcgcaccc gatacactac
       61 tggacggcgg cggtattgat tacactgaaa ttggcgcgat tcgctgggct tttgctatcg
      121 ccccggatca cccgttagcc tttgtgccgg aacccatcgc cgaaagccaa ctgcgtctct
      181 accctaatat tatggtggag gacaccgcgc atacgattaa taaaaaagtg ggctggttgc
      241 tgcacgggca ggagtcaatt ctggtgccag atttcaacac caaatgtcag tgccagattt
      301 taggtgaagg aattggtttt ttgccggatt acatggtccg tgaggcgatg acgcaatccc
      361 tgctggttac ccgacaaatc cataatccgc gccaggattc gcgcatgtta ctggcgactc
      421 agcattctgc tacgggtcag gtgacgcagt ggattaaaaa acaatttgcg ccaaatggca
      481 tattaacggg gatctatcag gatttactgc atcgggagaa ttaatcgctg ccaacctccc
      541 gatgcgctgt gaatagcggt ttacatcaac cacctgcttt cattacccat tcagagataa
      601 atttattacc cggtactggc ggagagtaca aatagccctg caaaaagtga acgcctttac
      661 cgatcattaa atccgcctgc tcctgggttt ctaccccttc cgccacgata ctcagaccaa
      721 gcttacgcgc tagttcgaca atattgtcca caatatgacc ggagatttcg tcgacactcg
      781 ccatttgcac aaatgactta tcgatcttaa taaaatcgac cgggaacgcc tgcaagtaac
      841 gataggtcgc ataacccgta ccaaagtcat ccagcgcaaa ggtaatgttg tgctgatgaa
      901 ggctgtcaaa tatcgctctg gcttctggcg ttaccggaat agggttacgt tccgttagct
      961 cgagaaccag cttgatttta tcgttaccta atttcttaac caggttcaga cactcttttt
     1021 caaatcccgc tgccaaaaaa caacccgccg agacgttgat gccaatatgg aaattgtccg
     1081 gcagcaaatg ttttaccggc atcagaatat ccgcagtctg tttcatcagt tggcgggtca
     1141 ttatgacaat aagaccggat gactccgcca gaggaataaa ctgatccggt gggataattc
     1201 ccgtttgtgg atgttcccag cggacaagta cctcacagcc cgtcagtacc ccagtctgcg
     1261 cgcagaaaac cggttggatc cacggtttga attcgtggtt ttccaacgcc agactgatgg
     1321 cttcaggcgt gacgatatgg tgacaacggt tatcagggat ataaaaatta tttttatttc
     1381 ccgtgccact gatgacttgc ggatgatatt gaaaaaagat atcccgccag aaggtaatat
     1441 cactctggat gcctagtttt tcatacagct gcttcttttg atgtgaaatt gtctttgcac
     1501 tgcgatttct gtactgtgag atttctgtaa ccgacattcc ctccattagc agcttgagta
     1561 aaagccgttc tttttcggac acgataccag gcaacgtcaa attcaccgtt gccccgaact
     1621 cttccaaaaa aacactcaaa tcatgcgaag ccatcgaaca caccttgatt aaatcttgtg
     1681 cgccagctaa atctgcgagt cttcactctg actgataaag ctggctgcaa gaaaatgaca
     1741 cagagagccg aattatctcc cgttcggctc tctctccttt tactcattat tcagatcaga
     1801 attgccactt aatgccaacc attgcagagg tgtcattata tcccctatcg gcaacctgaa
     1861 cgcccacatt cccccagaca ttcaggttgg catttagctg tccttccacc ccggttttta
     1921 tctcagcaat atttcgggct ccatcctgag tgacggacac gccatccata ctggtgctga
     1981 aatccttact gttatgcagc cagttcactt ctacaaacgg ctggaactcg cgggatttac
     2041 cgtcatccat tttatggtgg ctcttcagcc aggtttttac gccaagtcgg gtttgaacat
     2101 tgccatcacc gttgctatga acgagggttc cgttgctttc gcggtgctta tcggctttga
     2161 ctcccatcca ggtaacctgt gcttgcggct gaacatacca ttcattacgc gttccctggc
     2221 tgccattaaa ttcagctaat ttgtgtttgt atccagcttc cagtgaagcg gtaaatcctt
     2281 ttgatttata ggattcactt tgtaagtcat cccctttcac tgtgttatca aaccagctgt
     2341 actgcgccca actgtcgaga tacgcgccat tacgcgactc gtcatcggca taccaggtgg
     2401 catagaggcc ggtgctatat ccgttcacac tcgcttttgc acgataaccg gttcgcgagg
     2461 aaatggtttt actgtcgctg ttgccatatc ccgccatgac cccaacatgc cagctgtcgc
     2521 tgccgttttg gctccactgc gcgacatcgc ctcccagttg cagaacatag cgattgcttt
     2581 gggttttcag ctggccgctg ccatcacgcc atttattatg accaccttca tggcgcatcc
     2641 acatagtggt ttgtttctgc tcacccgtca ccatgtcggt atagtacgta ttacccagac
     2701 gctcatgcag acgcgtggtg aacattgtat tcgctgctgc aaggttcgca atgtagctac
     2761 ccgcctccgg tcgcaggtca ttatccacat tcagatccgg acccggcgta ggtgtcgggt
     2821 tagggttcgg ctctggattc gggtttggct ccggattcgg catcgggtct ggctccggct
     2881 gcagttcagg agaatcactg ccgctggtca gataccagtt accactattt gccccttgtc
     2941 cacgcgcgag agtgtagtca tacgcccccg caacgatacg accagcctga acaaattcgc
     3001 cctcagactt accgtctaca tggataagtt cgataccgtt aagggttttc gcacctgtac
     3061 cgccagcgtt attcaccgta acggcagtcg tgccgctagt atcgccctcg acaaccaatt
     3121 tatcggttac tgagtcatcg ccattcagta ccgtattcat tactagttga ccgttattac
     3181 cgtggtagtt ccccttcacc actaacacat tgccagcttg ctgtcctgac attccggtca
     3241 tcagcgtacc gttgttggtc agattgccgc ccagggtgta attcacagac gcagccgctc
     3301 ttttttcagc gctattattc aacgccaggg taccgttgtt ctcaacgttt gcgctaccca
     3361 atccctgcgc atgacttacc aggacattag ccgaattagc aatgttcaca ttcgctgtca
     3421 ggttgctgtt atcgcccgcg atgtcaagcg tgccgccctg aatattcagc tctccgctcc
     3481 ccgttaacga accggttgaa gtgcccccgt tggtaaccgt cagactgccg ccatttaagc
     3541 tcagcagtga atcggcgctg ccattgagcg tgcccacggt ctggctgtga ccattcatat
     3601 ccagttcagt ctctgccgcc agacgcagtt catgggtatt acccaacacg ttatcgttat
     3661 tcaacaaaag cgtcccactg cgcagatcgg taacaccggt atagtcgttg tctttgttag
     3721 aaagcgatac ggtctgaccc ttctggctgc tgaatgccag gtcaccactg ccggtaatct
     3781 ttgcgctgag atcggcggca ttctcgcttt taccgttaga gctcagcacc agagcatcgc
     3841 tgtcggtagc gtgtaaatca agctgggtca ggccgtaacc gatatacaga ccgtcattgt
     3901 ttaccccact cgtcagacga tagtcgtaag tgccgacggc cgctttttgc ccattctgct
     3961 gaatatcacg ttgggcactg ctggagagga tttgcccatt tttatcctgc aattgcagat
     4021 tgcccgcatc gcccagaact tccgcacctt gcgcatcaac caacttaatg gtgctgttac
     4081 catcatcgac ttcagtgagc gataacgcag agtcaatatc acgagatact gagcttacaa
     4141 cgtcactgtc agaaacctga atcacaccct cgccgcgcaa atccagcgtt ttactaacgt
     4201 tgaccgtccc ttctgtcatc tgtgcacctg ctgtcagggc accaaaatca acggttccgc
     4261 cggagaatcc caaagtgcta atggagtgag cgcccttacc cactgttacc aggcttccct
     4321 gacccgcagt gatcccgcct ctctgcagaa actcagcatt cttttccagg ttaaatgtcg
     4381 tgttctggaa cgccagtgaa ccctggaacc agtctgtata agcaaactca tcgaaggaga
     4441 attcgtgatt ccccatgttt atttcaacaa tacctctacc ttgaagatcg ttatcaaata
     4501 cccaatcttt attggcattg atttgcagtt tgccatcaac atccaggtcc gcacggccga
     4561 tgttcttgtc ttcagaaata accgcgacag caccagaagc gacatcaata tatccgctga
     4621 atgcatagtt attgccggtc agtgccactg tcccactgtc gatacttatt gtaccattgc
     4681 cggaaatctt attggcaaag gtcatgctat cgtcagcctt caggtgtaat ttcccgcggt
     4741 tgttgatctc tgctgtacct atatttttgg tgatccctaa agcgccattt tgctcaattt
     4801 tgactttacc actaaaggca gagttatcac cggttaagta agtcgtgcca ttgagcgcat
     4861 tgatatagcc caggccctcg ctgccactga caatatccgc ggaaatagtc gcgtttttat
     4921 ccgtgtggtt taaggtgatt ttcttatcgg atgtctttgc ccacacaaac tcaatttttt
     4981 cggcatcaat aatccctgca gccttcgcag cacttccttc ctgtgcacct aacgctaact
     5041 cagagttggt gcttaaacta attttcggtg cggatatttt acctccatct gatacgacta
     5101 atgttgtatc agaacgttgg tcgtaataac ccaggctaat atgttcacga gcattaactt
     5161 caccaccgtt attaaccgac aggaactgat taccgccata tccgccgtaa acgcccataa
     5221 agaaggtccc tgcatttacc tgactacctt cgccgcttac attcccctgc gtataagtca
     5281 gacgactggt gcccttaaca tgaattttgc cgccattgga gacattaaaa tagccttgat
     5341 catgtctcat gcctgagttt gactcataca aatcatcagc atataactcg ctgccagcat
     5401 tggagacatt caggaacccc gtacctttca gcaaaaagtt aagcgggcca ttgattgtta
     5461 cctttcctcc ggtaatatcc aacccaccac cagaagcgag agtcagacgc ggacgggaag
     5521 tatctgtcgt attgattacc agattggcat tgtcagtcac cgtaatacgg taatcgatgc
     5581 tctggttctc tttgccaaaa tcaatagctt tatcctggtt tacgatgaca tcatcggcga
     5641 ataccagaga tgaatataaa cttatcgcgc taattaaaac agcccgggac ttttttcctt
     5701 ttactctgct ccccagctcc gagcagacgc tccaacagtt acgggacctg ttccacttta
     5761 gccgatatat tttattcatc acaatctcca gcaatacatc caaataaaac caagattgca
     5821 taaacagcaa cgcattaaat gccattctgg ttactgttca tcgcatataa aaattcaaaa
     5881 attttgcata gcattggcaa ccacatatct gagtatgtag ttcagagggt aataaaatag
     5941 caagttacta ctgaaaactt attttaagtt aacactctta actttatcat tacattcctc
     6001 ccagtctcta atacctgcgc attatatgta tccaggaact caaatcaact caaaatataa
     6061 acaaaagcag gaggaataca tttaatatac agacggtaaa agtggtaact cttattaggt
     6121 atccaaatat aagaatcgtc ttattaataa atatatgact ctataggact gaatccattc
     6181 agctaataca aaataaaatc gttaattaat aaaatataaa gtcatgaagt gatacaagag
     6241 gaatttcagg atatgacatg acaatcatat a
//
LOCUS       DAZGAN020000189         6248 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGAN020000189 DAZGAN020000000
VERSION     DAZGAN020000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.189"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..282)
                     /locus_tag="ACHKYO_RS23870"
                     /old_locus_tag="pgaptmp_004774"
     CDS             complement(<1..282)
                     /locus_tag="ACHKYO_RS23870"
                     /old_locus_tag="pgaptmp_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HGU4431463.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMM"
     gene            complement(279..1949)
                     /gene="menD"
                     /locus_tag="ACHKYO_RS23875"
                     /old_locus_tag="pgaptmp_004775"
     CDS             complement(279..1949)
                     /gene="menD"
                     /locus_tag="ACHKYO_RS23875"
                     /old_locus_tag="pgaptmp_004775"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HGU4431464.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     STFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTRDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2038..3333)
                     /gene="menF"
                     /locus_tag="ACHKYO_RS23880"
                     /old_locus_tag="pgaptmp_004776"
     CDS             complement(2038..3333)
                     /gene="menF"
                     /locus_tag="ACHKYO_RS23880"
                     /old_locus_tag="pgaptmp_004776"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HGU4431465.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     MREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3412..3717)
                     /gene="elaB"
                     /locus_tag="ACHKYO_RS23885"
                     /old_locus_tag="pgaptmp_004777"
     CDS             complement(3412..3717)
                     /gene="elaB"
                     /locus_tag="ACHKYO_RS23885"
                     /old_locus_tag="pgaptmp_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HGU4431466.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3772..4233)
                     /locus_tag="ACHKYO_RS23890"
                     /old_locus_tag="pgaptmp_004778"
     CDS             complement(3772..4233)
                     /locus_tag="ACHKYO_RS23890"
                     /old_locus_tag="pgaptmp_004778"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4431467.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            4298..5215
                     /gene="rbn"
                     /locus_tag="ACHKYO_RS23895"
                     /old_locus_tag="pgaptmp_004779"
     CDS             4298..5215
                     /gene="rbn"
                     /locus_tag="ACHKYO_RS23895"
                     /old_locus_tag="pgaptmp_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HGU4431468.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTTFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGMLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            5403..6248
                     /locus_tag="ACHKYO_RS23900"
                     /old_locus_tag="pgaptmp_004780"
                     /pseudo
     CDS             5403..6248
                     /locus_tag="ACHKYO_RS23900"
                     /old_locus_tag="pgaptmp_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
BASE COUNT         1574 a         1672 c         1477 g         1525 t
ORIGIN      
        1 catcatcgcc acccgtccgc caagcgagta ccccaccagc caaaagttaa ggatgttgta
       61 actaaccaag gttttacgca gtaagtcggt gacatcatca aatccatcga cgctaatcgc
      121 cgccgaacca ccatgacctg ggagatcaac atacaaccgt gagtagtcgg caaacgcctc
      181 gcccacttct tgccattcgt ggcaatcgcc ggaaaaaccg tgcaaaaaca ccagccaggg
      241 taaacctggt tttccgtgtt ttgcctgcgc gtgcaggatc ataaatggct tacctgcgcc
      301 agaagttgct ggagcgtttg cgcaccatcg gtgtcgttaa ccaccatttc aatcaccgtg
      361 gtggttggcg tgcgccaggc gtcggcaaat gccgtttcaa gttcctgcca gttttgcgga
      421 cgatgatatt tcagctcgaa catcgcggcg gcgtgctcaa aatggacgtt ttgcggcatc
      481 agatagaaac gctcacgctc gctttgcggc gttggcaaca gcgagaaaat ttgcccgccg
      541 ttgttgttca ccacaattaa taccagcggc gcagaaacct gacgcaataa cgccagcgcg
      601 ttgagatcgt aaagtgcgga gagatcgccc acaatcgcca gcgtcggttt gccgcttgcc
      661 cgctgaacgc ctgcggcggt cgaaagcagt ccgtcgatac cgctggcccc tcggttgctg
      721 tacaccgggt aacctgccgg aagttgcgaa agcgcatcaa tcagacgtac caccaggctg
      781 ttaccaacaa acaattgccc ctgttcaggc agatagtcgc agatgcgatg cgccagttgc
      841 gcttcgccaa acgcatcacg gcgggcaata accgcctgca ttgcctgttc cgccaggcgc
      901 gggatttcaa cgcaccaggg ctggcgtttt tctgccggat gcagctccag ccagtcggca
      961 atattggcaa ttaagcgacg tccgcgatgg tgtgccggat caagtcgccc ttcaatgtca
     1021 tcaacaatcc agtactcttc tggttcacag cttgcctgcc attgcaggag ccgtttgccc
     1081 gtcaggctgc ttcccagttg caccacaatt tgcgcctgct gcagctcgct ggtcgctttg
     1141 gcattgccta accaaagatc ggcacacggc agcggctgcc cggtttgtga cagcacatcg
     1201 ccaatcagcg gccagccaag agtttgcgcc cacagggcaa cttttttgcc ctcttccgca
     1261 ctcatgcgcc cggcaaccac cacgccgcgc ttttgtcgcc agaagaacca gtcgcgctgt
     1321 ttttcacttt ccagacgagg cgcttcacgc agccacggtt tgtcgtcctg ccaccagtca
     1381 cccagacgct gttgccagct aagcccggta tcgtccattt cgccatacag cggttcagca
     1441 aacgggcagt tgatatggac tccccccgca tgaagcgtac cgagagcgtg gtcgatggta
     1501 gaaaccagcc aacgtgcggg gatatcccgg gtcgggcgcg gcaatgaaat actgtgcgtg
     1561 gggtgagagg cgaacattcc cggctggcga attgcctgat tcgcgccgca gtcaattagc
     1621 tccggcgggc gatcggcggt taacaggatc agtttttctc cggttaaccc agcttcaatc
     1681 agtgccggat agagatttgc taccgccgtg ccggaggtca caatcaccgc caccggctgc
     1741 ttgctgactt tcgccagccc cagcgccaga tgtcccagtc cacgctcatc gaaatgggtg
     1801 tggtgaatga atgtggaatt ctccgccgcc gctaacgtta acggtgtaga acgcgagcca
     1861 ggggcgatac agatgtgtct gacgccgtga cgcgttaatg cttccagaat gaccgccgcc
     1921 cagcgtcggt taaatgcgct tactgacatg agtttgtccg gtatcaatat tgcggctaag
     1981 tataaggagc tacaaaaata gattattgat atgaatcggt aatgatgcga ctcattacta
     2041 ttccatctgt aataaagtac gcagccctgc cgctttgttg tcgatttcct gccactcttg
     2101 ctcggggtcg gaaccacgga caatgcccgc gccagcatat aatcgcacga cattgccgct
     2161 aatttttgct gagcgcaggg aaacgcagaa ttcgctttgt tgtaatgaga gatagcccgc
     2221 agaaccggcg taccattctc gggtgaacgg ttcgtgacgg gcgataaact gtcgcgccag
     2281 atcgcgcggt aagccagcaa ctgctgccgt tggctgcaac tgatgtaaac agatcacatc
     2341 atccgctttg ttgagtgaag tccagataca gcggcgaaga tgctgcactt tacgcagacg
     2401 cagtacctgc ggcggtaaaa catccagcgt ctgggtatcg gcctgtaatc gttggcagat
     2461 atcttccacc accagcatgt tctcgcgctg gtttttatca tccgccatca gccactctcc
     2521 taactgctgc gcctgcttat catcaggatt atttgctact gttcccgcca gcgcttcagt
     2581 acgcagcgct ttgtcacgcc gccgccataa ccgttccggt gaagagccaa gaaaagcatt
     2641 ttcgccatca aaggccatgt aaaaatggta gcaattcaga ttcagtcgac gactggcagc
     2701 catcatcgcc gccgcgttga ccggacttgc gaaatgcagg tcagttgccc gagcgagcac
     2761 cactttgtcg agctcacctt cggcgatggt tttcgttgcc agttcgatta attgcgtcca
     2821 gcccgttttg tccggccagt gttgttctcg catggtggtt aaatgtaacc caggcaaggg
     2881 cttgatactc accagtgtgg cgataaattc ttttgcctga atcgcatcgt gctgaaggga
     2941 gctttcgctg aatagcgtca gccgcagcgt ggctttaccg ccacagcgtc gccattccag
     3001 gcggggtaaa agtaaattgc cctgcgacgg gtcaaaagca ttcagccccc aaatgcgtaa
     3061 gtcggcgtgt tccgggtgct ggcgaagaaa acgttgtgcc tggtccaacg acgtaaaacg
     3121 ggtaatcgcg cccaggacgg cagcttcttc atcaccatta cgttgttgcc agtagaattg
     3181 cgggtacgtt tgctgactgg ccagccagct caaggcatca aaagcgtctt tgagagggaa
     3241 aggaatatcg ataacccgaa tgccgggtgt cgccggaatc tcttgcgaca aatggcgcag
     3301 tagattttcc agcgccgtag taagtgattg cacgcggacc tctccctgtt aaaagcctca
     3361 cattatacgg ggtactacaa aaaaaatgca gtaccccggt gtagggaggt tttaacggcg
     3421 tgccagcaac agtcctagta ccagcccaac ggccgcgccc acaccaattc cttgccaggg
     3481 tttttcgtgg acgtagtcat cagcacgata aactgcctgc ttcgcccgat agtaataact
     3541 gtctgaagcc tggctaaccc gttttttcac atcatccagc gctttttctg cacgcgcttt
     3601 cagctcaaca tatttctgat cggcgggatc gccagaggag cggagcacct cttccagtgt
     3661 ttcactaagc agcgtcaggt cgtcatcgat acgtgtatca ccaaactgat tagacattct
     3721 cgttctccat ttgcgtaaaa cctgtcacta actatagaca agggttgttg attacgcctg
     3781 aattacctcg cgtgccatcc caatgtgtgg gataccatct tcttcataga cctccgtcac
     3841 agggataaaa ccgaaactct ggtagaagtt ttgcagatgc gcctgcgccc cgaggtaaac
     3901 aggcttatca ggccagtgat gcgtacatgt ttcgagtgtt ttactcatca gttgctgccc
     3961 cactttttcg ccacgcaacg cttcactgac aatcacccga cctataacga ccggctcaag
     4021 atcatcatca cttttcagaa tcctcgcata tgccaccagt tcatcatttt tccaaccaag
     4081 aatatggcga ttatcccccg tcaaatcatc accatcgata tcctgataag ggcaattctg
     4141 ctcgacaaca aacaccgcgc aacgcagttg taataaggca taaagctggg aaaccgataa
     4201 ttcagagtga tgcagatctt gccattcaat cataatgctc tccttttaga tgtatgcctt
     4261 atactagccg gaatattttt tgaaacgtga tgaactcatg gaattaattt ttttaggtac
     4321 ttcggccggt gtgccaaccc gcacgcgcaa tgtcacggca atattgctta acctgcaaca
     4381 tccgacccag agcggacttt ggttgtttga ctgcggtgaa ggcacccagc atcagctact
     4441 gcataccacc tttaaccctg gaaaactgga caagattttt atcagtcacc ttcatggcga
     4501 tcatcttttt ggtttacccg gcttgctgtg cagtcgttct atgtcaggca ttatccaacc
     4561 cttaacgatt tatggtccgc aaggtatccg tgaatttgtg gaaaccgcgc tgcggattag
     4621 cggctcatgg accgattatc cgctggaaat tgtcgaaatt ggcgctggcg aaattctcga
     4681 tgatggcctg cgcaaagtaa ccgcttatcc gctggaacac ccactggaat gttatggcta
     4741 tcgtattgaa gaacatgata aaccgggtgc attaaatgcc caggcattaa aagctgctgg
     4801 cgtgccgcct ggtccactgt ttcaggaatt aaaagcgggc aaaacaatca ccctggaaga
     4861 tggaaggcag attaacggcg cagattactt agctgctcca gtgccaggta aagcgctcgc
     4921 tattttcggc gataccggcc cctgcgatgc cgcacttgat ctggctaaag gtgtcgatgt
     4981 catggtgcac gaagcgacgc tggatataac catggaagcc aaagccaata gtcgcggcca
     5041 tagctctaca cgccaggctg cgacactggc ccgtgaggct ggagtcggta tgctaatcat
     5101 tacccacgtc agctcgcgct atgatgacaa aggttgtcag cacctgttac gtgaatgcag
     5161 gtcaattttc ccggcgactg aactggcgaa tgatttcacc gtgtttaacg tttaactctg
     5221 cttattatta aaaacagggc gaaacttgcc ctgttatcgc aacccgcgct acatatatcc
     5281 acccacacgg gcatattgag cgatttttga tatttattca tctcctcacg cactatattc
     5341 agagcaataa gttgcctcta tttatcttga ttcagcacaa cctttgcaac taaaaggagc
     5401 ccatgatggt tacagttgtc agcaattatt gtcaattatc tcaaaagcaa ctcagtcaga
     5461 catttgcaga aaaatttact gtgaccgagg aattactgca gtctttaaaa aaaacagcgt
     5521 tatccggaga tgaagaaagc attgagttac tgcataatat tgcgttaggt tatgataaat
     5581 ttggaaaaga agctgaagac attctttacc atattgttag aaacccaaca aatgagaccc
     5641 tatcgattat ccgacttatc aaaaatgctt gtttaaaatt atataatttg gcacataccg
     5701 caaccaactc cccccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa
     5761 agctattctc cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg
     5821 aaaaacagtc gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta
     5881 caaacttctg ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga
     5941 agatcgccgc taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct
     6001 actccaccag aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat
     6061 ggccgaatga agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc
     6121 tacagaaaat agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc
     6181 attgcgtgca ctaggccatg ataaagaaaa ttcacttacg cgtgtcatta atagtttcaa
     6241 ttctgaac
//
LOCUS       DAZGAN020000190         6224 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGAN020000190 DAZGAN020000000
VERSION     DAZGAN020000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.190"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            5..790
                     /gene="rcnA"
                     /locus_tag="ACHKYO_RS23905"
                     /old_locus_tag="pgaptmp_004781"
     CDS             5..790
                     /gene="rcnA"
                     /locus_tag="ACHKYO_RS23905"
                     /old_locus_tag="pgaptmp_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HGU4431469.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHHEHGEYQDAHARAHANDIKRRFDGREVTNW
                     QILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLALTLITVGVGAAIS
                     VQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1009..1347
                     /gene="rcnB"
                     /locus_tag="ACHKYO_RS23910"
                     /old_locus_tag="pgaptmp_004782"
     CDS             1009..1347
                     /gene="rcnB"
                     /locus_tag="ACHKYO_RS23910"
                     /old_locus_tag="pgaptmp_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HGU4431470.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1429..2463)
                     /gene="yehA"
                     /locus_tag="ACHKYO_RS23915"
                     /old_locus_tag="pgaptmp_004783"
     CDS             complement(1429..2463)
                     /gene="yehA"
                     /locus_tag="ACHKYO_RS23915"
                     /old_locus_tag="pgaptmp_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HGU4431471.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     AIALINVKYD"
     gene            complement(2477..4957)
                     /gene="yehB"
                     /locus_tag="ACHKYO_RS23920"
                     /old_locus_tag="pgaptmp_004784"
     CDS             complement(2477..4957)
                     /gene="yehB"
                     /locus_tag="ACHKYO_RS23920"
                     /old_locus_tag="pgaptmp_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGU4431472.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQTYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(4973..5647)
                     /gene="yehC"
                     /locus_tag="ACHKYO_RS23925"
                     /old_locus_tag="pgaptmp_004785"
     CDS             complement(4973..5647)
                     /gene="yehC"
                     /locus_tag="ACHKYO_RS23925"
                     /old_locus_tag="pgaptmp_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4431473.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(5728..>6224)
                     /gene="yehD"
                     /locus_tag="ACHKYO_RS23930"
                     /old_locus_tag="pgaptmp_004786"
     CDS             complement(5728..>6224)
                     /gene="yehD"
                     /locus_tag="ACHKYO_RS23930"
                     /old_locus_tag="pgaptmp_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4431474.1"
                     /translation="SAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSINMPTVPTT
                     VFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIASSNDTVG
                     YYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVLSSLVMR
                     VAQD"
BASE COUNT         1673 a         1500 c         1233 g         1818 t
ORIGIN      
        1 tatcatgacc gaatttacaa ctcttcttca gcaaggaaac gcctggttct tcatccccag
       61 cgccatctta cttggtgcgc ttcatggcct ggaaccaggg cactcaaaaa cgatgatggc
      121 ggcgtttatc atcgccatca aaggcaccat taaacaagcg gtgatgctcg gactggcagc
      181 aactatttcg cataccgcag tggtctggtt aattgccttt ggcgggatgg tgatcagcaa
      241 gcgctttact gctcaatcag cagaaccgtg gctccagctg atttccgcag tgatcattat
      301 tggcaccgcg ttctggatgt tctggcgtac ctggcgcggc gaacgcaact ggctggagaa
      361 tatgcacgag tatgatcatg aacatcatca ccatcacgaa catggcgagt atcaggatgc
      421 ccatgcacga gcccatgcca atgacattaa acgacgcttt gatggtagag aggtcaccaa
      481 ctggcaaatt ttgttatttg gcttaaccgg tggccttatc ccctgcccgg cagcaattac
      541 cgtgctgttg atatgcattc agttgaaagc cctgacactg ggcgcaacac tggtcgtcag
      601 tttcagcatt ggcctggcgt taacgcttat caccgtaggc gttggcgcag caatcagcgt
      661 tcagcaggtc gcaaaacgct ggagcggatt taacactctc gctaaacgcg ccccctattt
      721 ttccagtctg ttgattggct tagtcggtgt gtatatgggc gtacatggct tcatgggcat
      781 aatgcgataa ctcaatcctt taggcttgca tgttatcttc agcccaggat gattcttaaa
      841 tcagctattt ccgctgactc tctgctcgct aaggatgctt ttagggcatc ctttcattta
      901 cactttttac gaaatcatgg gatcactaac aaaatatcgc ttgtcagtta tattgtatgg
      961 caggaaagat atgcgactga tattacagat ccccaaagtg gagagtttat gaccattaaa
     1021 aataagatgt tgctgggtgc gcttttgctg gttaccagtg ccgcctgggc cgcaccagcc
     1081 accgcgggtt cgaccaatac ctcgggaatt tctaagtatg agttaagcag tttcattgct
     1141 gactttaagc atttcaaacc aggcgatacc gtcccagaga tgtaccgcac cgatgagtac
     1201 aacattaagc agtggcagtt acgtaacctg cccgcgcctg atgccgggac gcactggacc
     1261 tatatgggtg gcgcgtacgt gttgatcagc gacaccgacg gtaaaatcat taaagcctac
     1321 gacggtgaga ttttttatca tcgctaaaaa aagccccctc atcatgaggg ggaaatgcag
     1381 acaccttgtt attttttatt attagccact tgctcgtctt gcttgttatt agtcgtattt
     1441 cacgttgatt aatgcgattg cctccagtgc gccagattta actttgtttg tatcgtagac
     1501 gtagtaactg gcagttatcg gaattgaaac cgttgtatct gtaagttttg actttgccgc
     1561 aatttctttt aaatttgtgt atcctttact gctgttagtg atatttaaaa gtgtgactga
     1621 cgaaccatta ctttcaagaa taaatcctac gccattatct tccgcacctt tcagtattgt
     1681 gttgctgttg gttaacaagt cagaacttga taaatacacc tgcattttcc gtgatgtttg
     1741 attttgttga cctaataaat ttttacaacg cagaataata tccccttgtt tactatttat
     1801 tgttgctttg ttacctgtgg ctggtaattg tgaaacggga atactgtcta aagtaattaa
     1861 taaatcgtcc ggtttgcagg ttgtggttat cgggttgtat gacaatgtca gattaaagga
     1921 atagacacct gcattttgtg ccaatgcatt tctgtagcta tttacacatg catcccatga
     1981 cttgccacct aataaacaca gtagaaattg tactgcggca ctcccgcgtg tcgatgatga
     2041 agcactggat aatgtcgcaa ctccattaat catcactgaa ccgccgcccc ccgcagaaac
     2101 attcactttt gcattactgg ccgcaggtgt caccgttacc gaataaccac tggtgaaact
     2161 ggtctgatta tttgataacg tgatgttgtt tttattggtc cattcaaaat gtaaatttaa
     2221 tttaatggta tcgttataaa gtccaacaat aatatccgac gggctcatag gggtgatagt
     2281 atttgtactc acgcatgacg aatcattaaa attatgctca tatgtcgcca tttgtgtttg
     2341 agtaagtgca aaatccccct gaacaaccaa attgtgctga acagataact cactataaca
     2401 tgccgcatag ctcacaggca taaccatcat cattactata aataacatta tacgaatatt
     2461 cataaccacc tgactattac tggcaaatat agtttctact ttcatcaatc tctttaccga
     2521 aggtgattgt gcatgaaagt ccttgttgct tatcaattgc tacattaacc gatggcggta
     2581 tttcattggt gcgaataaat aactggcttc cctggccgac aacgccaata ttatgaccgt
     2641 ggatatcatt gacttcataa ccaaacgtta atggttgccc atccgctctt aacgctttta
     2701 taaaccaggg cttgcgctga tcggtatcaa aattaaccag tacaaccgcg ccgcgataag
     2761 gtgcggcaat tttccggttg ccacgtaatt ctgcttcgct atcgctttgc gacacatcca
     2821 gcatcaggtg attttcccga taaggtgtca ttccgtcgta taccaccact ccattacggt
     2881 ttgttgtgcg atatttttgc ccattgacat aagcatcttt aattcctggc gcattcatca
     2941 cagcaaacgt ttcggaaaga cggttcgcca gattaacgcc ccccgaccag gcgacaatgc
     3001 cccctgaaac actggctcca gcctgtcgat aagtactcga ctgactataa ctgccattca
     3061 ctgtcgcaac cggcgcgttc caggtcaaat tcgccccagc tgtcgtttca tttccctgat
     3121 tctgataact caggttgacc ccatagttaa actgatcccg gcttccaacg gttcctgata
     3181 atcccgtatt atttgaggca aacccctgat catcaaacgt cgttgagtta gacatatata
     3241 tttgccgacg aggcgtcgta acgtcatcac cccaatcaaa gggaatcgat ataaaaatat
     3301 taaaacgttt ctcttcatga tgattctcgt catacgtctg gcttgccgcg aggatatagc
     3361 ttatccgccg ccagttattg gaataactca actgataatc cttactgctg ccgctacgcc
     3421 cccagtaatc tcgccataac gtacttaagg acacagaccc ccagccttct ggcaatgact
     3481 ggctcatatt ggcagagaag ctatttttac ggccaaaatc gttctggtaa taatcggcaa
     3541 tgtcatagac atcgttttca tcacggcgat aattatcttt attgtttgcc caaacgtgat
     3601 cattaaatgt ccggtaatca cgcgatgaat aacgccaggc cgccagacca aaacgcgtcg
     3661 acgtttggct cacaaatttg ttgtaggcaa tttgataact ttgcccgtca aacacatcgc
     3721 cgttgtcttg tttactatgc gacttcgtgg catcgacgga aatggcacca atgcgtgtat
     3781 tccagccagt ccccaaagta aacgcgtaat aattattcgc gaccatcgag ccaccataca
     3841 gcgtcaataa attattaaaa ccatactgat aacccgcctg gacaaaatca ctttgtttgc
     3901 tcgccccttc aatatggcta cgacccgccg caaaatcata tttcgacacg ccgggttgca
     3961 gcatatttgg caccgctgca taaggcacca gataggtggt taccgaaccg tccgcctctt
     4021 tcacgctgac atcaagatct gctccaccac cggccaactg caaatctgta atcgcaaacg
     4081 ggccaggagg aacctctttc tgataaacca caaaaccatt ctgttcaata gttaccagcg
     4141 cgttactctg agcaatcccc tgcacccgtg gcgtaaaatt ttgtttcgag ttaggcaaca
     4201 tctgcatatc acgaaacaac cgcacacctc tgaagcgaac agaatcaaaa atatcgcttg
     4261 atgtgtacat atcacccacg cgaagcgtgc cgagaagttg ggcaaatcca cgttccagat
     4321 acagggtatt gcttttccac acccctggat tgttatttgt tttactgaaa ctggcatcag
     4381 aatgcagttg ccaccccagt aaatttaacc cgctgttaaa acgtacatat gtactcttac
     4441 tattacccga cgctttatag tcactgtaat actgactcac ataataagag gtataaaacg
     4501 cattaatacc ccgctcccag ttttccggtg gaacatagcc actttccagt tcttccaccc
     4561 aggcctgcgg gacactgaaa tcgagacgaa aaaccccgat atcccaggta tagctcccac
     4621 cctgaacaag ttgctcaaat gttaaacatt gcttaccgct ggcgaagtta tcgctattaa
     4681 tgcctaaccg cttgataact tctcttgata aacatgtttc ttgcgggttg tctttaacaa
     4741 taatctcata tttcccgcgc cattgcttat tgacataaat atcaatgtca tactgccccg
     4801 gtaagggttg attatcatca agacgaatat ttgcaacctg ctggtctttc attccaccta
     4861 tcataaaatg ggtatcaaag gtttcttcag ctgcataagc ttcaatgccg agcaataacg
     4921 ctacgattgc tgatgcaagt ggggtcattc tcaacatagg cagctcctgc aattaaattt
     4981 tgtcactaat atagttgccg tggtcatcga taatggtcag ataccagtta tttgcattat
     5041 tacttttgac attaacactc tgactttcta agggggcaat cattatagtt tcataattga
     5101 ctttaacatt attagctttg acatccgaaa tcgtcaccca attagctgag tcatttttta
     5161 tcaccaatcc attgccactg cgatttacca gtaacttttt aaaggtcgct ttatttaccg
     5221 gagcaatacc cgctggccgg taaaacaact taattctgtt ttgcatcgca aacttcagtg
     5281 catttttgcc ttcttgctct ggactatttg gtggaatatc cagaacattc agataaaaaa
     5341 tgctttcttt attagtgggc agtttattcg gcataatttt gatttttact tgctgcccgg
     5401 aatttgcccc tatttttgcc actggtggcg ttaacatgaa aggaacctga attttttctg
     5461 gtggtaacga cgtatcgcca tcatcaatcc acgcctgcag cagcgaagaa cggtttccct
     5521 ggttcatcaa ctgcaccata acttctttat tttctgccgg gtaaataatg cgcgtcccgt
     5581 agataacaat tccggcatgt gcaggaagga ccatagaaaa aatgagtaaa gataataatc
     5641 ctttcatctt aatgcctctt aaattaaaaa gccgccatgg ggatagcggc catgtttatt
     5701 acctggagaa acttacaatt ttatttatta atcctgcgcc acacgcataa ccagagaaga
     5761 taacacatca cctgattgca ctgagttatc tgtcagtttc aggtattttg ctttaaacgg
     5821 aatagcatat tgaccttccg ctgttttata actctcaatg ttaaaaggtg cagaaacatc
     5881 caggacatcg ctgttatcac ctaaatagag atagtaaccc acggtatcgt tgctactggc
     5941 tatcgcctta tcgttaccgg ttattttatc gttggtaatc aacgagagtt ttacaccagc
     6001 aacttctttc gggcagctaa tttttaacat gctgctggtt acaccgactg catcatcata
     6061 ggtagaatat ttagctttac cttcaaagac agtggttggt acagtcggca tattaatact
     6121 tactgaatca ccacccgctt cgaatttaca cggagattct gcaatattcc ccttaatagt
     6181 taatgttccg gtatcaacgt ctgcagcaaa tactccagcg ctca
//
LOCUS       DAZGAN020000191         6178 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZGAN020000191 DAZGAN020000000
VERSION     DAZGAN020000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.191"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1620
                     /gene="hcp"
                     /locus_tag="ACHKYO_RS23935"
                     /old_locus_tag="pgaptmp_004787"
                     /gene_synonym="priS"
     CDS             <1..1620
                     /gene="hcp"
                     /locus_tag="ACHKYO_RS23935"
                     /old_locus_tag="pgaptmp_004787"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HGU4431475.1"
                     /translation="TPAGNGCSYAQGMCGKTAETSDLQDLLIAALQGLSAWAVKAREY
                     GIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQCLAVDANARVDN
                     PMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLKGAAAYMEHAHV
                     LGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSILDAGETGKYGHP
                     TPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLPAHGYPELRKFK
                     HLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRSIVGWPGVRHLD
                     GEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVSREKLRHIFLLG
                     GCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLPRLVDAGQCNDA
                     YSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVKNIVTGPTAPGF
                     LTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            1632..2600
                     /gene="hcr"
                     /locus_tag="ACHKYO_RS23940"
                     /old_locus_tag="pgaptmp_004788"
     CDS             1632..2600
                     /gene="hcr"
                     /locus_tag="ACHKYO_RS23940"
                     /old_locus_tag="pgaptmp_004788"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HGU4431476.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            2733..4451
                     /gene="poxB"
                     /locus_tag="ACHKYO_RS23945"
                     /old_locus_tag="pgaptmp_004789"
     CDS             2733..4451
                     /gene="poxB"
                     /locus_tag="ACHKYO_RS23945"
                     /old_locus_tag="pgaptmp_004789"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HGU4431477.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            4488..5489
                     /gene="ltaE"
                     /locus_tag="ACHKYO_RS23950"
                     /old_locus_tag="pgaptmp_004790"
     CDS             4488..5489
                     /gene="ltaE"
                     /locus_tag="ACHKYO_RS23950"
                     /old_locus_tag="pgaptmp_004790"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HGU4431478.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            5500..>6178
                     /locus_tag="ACHKYO_RS23955"
                     /old_locus_tag="pgaptmp_004791"
     CDS             5500..>6178
                     /locus_tag="ACHKYO_RS23955"
                     /old_locus_tag="pgaptmp_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU4431479.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVS"
BASE COUNT         1406 a         1655 c         1739 g         1378 t
ORIGIN      
        1 actccggcag gaaacggctg ctcatacgcg caggggatgt gtggtaaaac ggcggaaact
       61 tctgaccttc aggatttact catcgcggcg ctgcaagggc tttctgcctg ggcggtaaaa
      121 gcgcgtgaat acggcatcat caaccacgat gttgacagct tcgcgccacg tgcctttttc
      181 tcaaccctga ccaacgttaa cttcgattct ccgcgtattg tcggctacgc tcgtgaagcg
      241 attgccctgc gcgaggcgct gaaagcgcaa tgcctggctg tagatgccaa cgcgcgcgtc
      301 gataacccga tggctgacct gcaactggtg agcgacgatc tcggcgagct gcaacgtcag
      361 gcagcagaat ttacccctaa caaagataaa gcggcgattg gcgaaaacat tctcggcctg
      421 cgtctgctgt gcctgtatgg cctgaaaggt gcggcggcct atatggaaca cgcgcatgtt
      481 ctcggtcaat acgacaacga tatttatgcc cagtaccata aaatcatggc gtggctgggg
      541 acctggcctg ccgatatgaa cgcgcttctt gagtgttcaa tggaaatcgg ccagatgaac
      601 ttcaaagtga tgagcattct ggatgcaggc gaaaccggta aatacggtca cccgacgcca
      661 acccaggtca acgtcaaagc gacggcgggt aaatgcattc tgatttccgg tcacgatctc
      721 aaagatctct acaacctgct ggaacagacc gaaggcacgg gcgttaatgt ctacacccac
      781 ggcgaaatgc tgcctgcgca tggctacccg gagctgcgta aattcaagca tctggtcggt
      841 aactacggca gcggctggca gaatcagcaa gtagagttcg ctcgtttccc tggccccatc
      901 gtgatgacct cgaactgcat catcgaccca accgtaggcg cttatgacga tcgtatctgg
      961 acccgcagca tcgttggctg gcctggcgtg cgtcatctgg atggcgaaga tttctctgcg
     1021 gtcatcgccc aggcgcaaca aatggcgggc ttcccgtaca gcgaaattcc tcacctgatc
     1081 accgtgggct ttggtcgcca gacgctgctt ggtgcagccg atacgctgat tgatctggtt
     1141 agccgtgaaa aactgcgtca tatcttcctg cttggtggct gtgacggcgc acgcggcgag
     1201 cgccactact tcaccgattt cgccaccagc gtgccggatg actgcctgat cctgaccctc
     1261 gcctgtggta aatatcgctt taacaaactt gagtttggcg atatcgaagg tctgccgcgt
     1321 ctggtagatg caggtcaatg taacgatgct tactcagcaa ttattctggc tgtcactctg
     1381 gcagagaaac tgggctgtgg tgtgaacgat ctgccgctgt cgctggtgct ctcctggttt
     1441 gaacagaaag cgatcgtcat tctgctgacg ctgctttctc tgggcgtgaa aaatatcgtc
     1501 accggcccga ctgcacctgg tttcctgaca ccagacctgc tggcggtgct gaacgagaaa
     1561 ttcggcctgc gttctatcac cactgttgaa gaagacatga agcaactgtt gagcgcgtaa
     1621 ggagtttgtc gatgacgatg ccaacgaatc aatgcccgtg gcggatgcag gttcatcaca
     1681 ttacgcaaga aacgccggat gtgtggacga tttccctgat ttgccacgat tactacccat
     1741 atcgcgccgg gcaatatgca ctggtcagcg tgcgaaactc agcggaaacg ctgcgtgctt
     1801 acaccatttc ctccacgcca ggcgtgagtg aatatatcac cctgaccgtg cggcggattg
     1861 atgacggtgt cggctcccag tggctgacgc gcgatgtaaa acgcggtgat tatctctggc
     1921 tttcggacgc gatgggggaa tttacctgcg acgataaagc agaagataaa ttcctgttgc
     1981 tggcggcagg ctgcggcgtc acgccgatta tgtcgatgcg tcgctggctt gcgaagaacc
     2041 gtccacaggc cgatgtgcgg gtgatctaca acgtgcgtac gccgcaggat gttattttcg
     2101 ccgatgagtg gcgtaactat ccggtaacgc tggtggcaga aaataacgtt accgaaggct
     2161 ttatcgctgg tcgtctcact cgcgaactgc tggcaggtgt acctgactta gcttcacgta
     2221 ccgtgatgac ctgcggcccg gctccgtata tggattgggt agagcaggaa gtgaaagcgc
     2281 tcggcgtgac gcgtttcttt aaagagaaat tcttcacccc agtagcagaa gcggcgacca
     2341 gcggtctgaa attcaccaaa ctgcaaccgg cacgagaatt ttacgccccg gttggcacca
     2401 cgctactgga ggcgctggaa agcaataacg ttccggttgt cgctgcctgc cgtgcgggtg
     2461 tttgcggctg ctgtaagaca aaagtggttt ccggtgaata tacggtgagc agtacaatga
     2521 cgctgaccga cgccgaaatc gctgaaggtt acgtactggc ctgctcctgc catccgcagg
     2581 gggatttggt tctcgcataa tcgccttatg cccgatgata ttcctttcat cgggctattt
     2641 aaccgttagt gcctcctttc tctcccatcc cttccccctc cgtcagatga actaaacttg
     2701 ttaccgttat cacattcagg agatggagaa ccatgaaaca aacggttgca gcttatatcg
     2761 ccaaaacact cgaatcggca ggggtgaaac gcatctgggg agtcacaggc gactctctga
     2821 acggtcttag tgacagtctt aatcgcatgg gcaccatcga gtggatgtcc acccgccacg
     2881 aagaagtggc ggcctttgcc gctggcgctg aagcacaact tagcggagaa ctggcggtct
     2941 gcgccggatc gtgcggcccc ggcaacctgc acttaatcaa cggcctgttc gattgccacc
     3001 gcaatcacgt tccggtactg gcgattgccg ctcatattcc ctccagcgaa attggcagcg
     3061 gctatttcca ggaaacccac ccacaagagc tattccgcga atgtagtcac tattgcgagc
     3121 tggtttccag cccggagcag atcccacaag tactggcgat tgccatgcgc aaagcggtgc
     3181 ttaaccgtgg cgtttcggtt gtcgtgttac caggcgacgt ggcgttaaaa cctgcgccag
     3241 aaggggcaac cacgcactgg tatcatgcgc cacaaccagt cgtgacgccg gaagaagaag
     3301 agttacgcaa actggcgcaa ctgctgcgtt attccagcaa tatcgccctg atgtgtggca
     3361 gcggctgcgc gggggcgcat aaagagttag ttgagtttgc cgggaaaatt aaagcgccta
     3421 ttgttcatgc cctgcgcggt aaagaacatg tcgaatacga taatccgtat gatgttggaa
     3481 tgaccgggtt aatcggcttc tcgtcaggtt tccataccat gatgaacgcc gacacgttag
     3541 tgctactcgg cacgcaattt ccctaccgcg ccttctaccc gaccgatgcc aaaatcattc
     3601 agattgatat caacccagcc agcatcggcg ctcacagcaa ggtggatatg gcactggtcg
     3661 gcgatatcaa gtcgactctg cgtgcattgc ttccattggt ggaagaaaaa gccgatcgca
     3721 agtttctgga taaagcgctg gaagattacc gcgacgcccg caaagggctg gacgatttag
     3781 ctaaaccgag cgagaaagcc attcacccgc aatatctggc gcagcaaatt agtcattttg
     3841 ccgccgatga cgctattttc acctgtgacg ttggtacgcc aacggtgtgg gcggcacgtt
     3901 atctaaaaat gaacggcaag cgtcgcctgt taggttcgtt taaccacggt tcgatggcta
     3961 acgccatgcc gcaggcgctg ggtgcgcagg cgacagagcc agaacgtcag gtggtcgcca
     4021 tgtgcggcga tggcggtttt agcatgttga tgggcgattt cctctcagta gtgcagatga
     4081 aactgccagt gaaaattgtc gtctttaaca acagcgtgct gggctttgtg gcgatggaga
     4141 tgaaagctgg tggctatttg actgacggca ccgaactaca cgacacaaac tttgcccgca
     4201 ttgccgaagc gtgcggcatt acgggtatcc gtgtagaaaa agcgtctgaa gttgatgaag
     4261 ccctgcaacg cgccttctct atcgacggtc cggtgttggt ggatgtggtg gtcgccaaag
     4321 aagaattagc cattccaccg cagatcaaac tcgaacaggc caaaggtttt agcctgtata
     4381 tgttgcgcgc aattatcagc ggacgcggcg atgaagtgat cgaactggcg aaaacgaact
     4441 ggctaaggta aaaagggtgg catttcccgt cataataagg acatgccatg attgatttac
     4501 gcagtgatac cgttacccga ccgagccgcg ccatgctcga agcgatgatg gccgccccgg
     4561 ttggggacga cgtttacgga gacgacccta ccgttaatgc tctgcaggac tatgcagcag
     4621 agctttccgg taaagaagcc gccatttttc tgcctaccgg cactcaggcc aacctggtcg
     4681 ctctgctcag tcactgcgaa cgtggcgaag agtatattgt cggtcaggcc gcgcataact
     4741 atctgtttga agccggtggc gcagcggtgc tgggcagtat tcagccgcaa cccatcgacg
     4801 cggctgccga cggcacgcta ccgctggata aagtggcgat gaaaatcaaa cccgacgata
     4861 tccatttcgc ccgcaccaaa ttactcagtc tggaaaacac ccacaacggc aaagtgctgc
     4921 cgcgtgaata cctgaaagaa gcatgggaat ttacccgcga gcgcaatctg gcgctgcatg
     4981 ttgacggtgc gcgcatcttt aatgccgtgg tggcttacgg ctgcgaactg aaagagatca
     5041 cgcaatattg tgattcgttc accatttgcc tgtcgaaagg tcttgggacg ccagtcggtt
     5101 cattactcgt cggtaatcgt gattacatta aacgtgccat ccgctggcgg aaaatggccg
     5161 gtggcgggat gcgtcagtcc gggattctgg ctgccgccgg gatgtatgcc ctgaaaaata
     5221 acgtcgcacg gttgcaggaa gatcacgaca acgccgcctg gatggcggag caactgcgtg
     5281 aagcaggcgc ggatgtgatg cgtcaggaca ctaatatgct gtttgttcgc gtcggggaag
     5341 aaaatgctgc cgcgttaggc gaatacatga aagcgagaaa cgtgctgatt aacgcctcgc
     5401 cgattgtccg cctggtgacg catcttgacg tctcgcgcga acaactggcg gaagtcgccg
     5461 cccactggcg cgcattcctg gcgcgttaag gagagagacg tgccgcaacg cattttagtt
     5521 ctcggtgcca gtggctacat tggtcagcat ctggtgcgca cactcagcca gcaagggcat
     5581 cagatcctgg cggcggcacg tcatgtcgac aggcttgcaa agctgcaact ggcaaacgtc
     5641 agttgccata aagtcgatct cagctggccg gataaccttc cggccctgtt gcaggatatc
     5701 gataccgtct attttctggt gcacagcatg ggcgaaggcg gcgattttat cgctcaggag
     5761 cgccaggtgg ctctcaacgt ccgcgatgcg ctacgtgaag taccagttaa gcaattaatc
     5821 tttctcagtt cgttgcaggc cccgccacat gagcagtcgg atcatctgcg tgctcgtcag
     5881 gctacggcgg acattcttcg tgaagcgaat gtacctgtga ccgaacttcg ggccggaatt
     5941 atcgttggcg caggttcagc ggcgttcgaa gtcatgcgcg atatggtcta caacctgcca
     6001 gtgttaacgc cgccacgctg ggtacgttca cgcaccacgc ccatcgcgct ggaaaacttg
     6061 ctgcactatc tggtggcgtt gttagatcat ccggccagcg aacaccgcat cttcgaagcc
     6121 gccggaccag aggtgctcag ttatcagcaa cagtttgaac attttatggc ggtgagcg
//
LOCUS       DAZGAN020000192         6141 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGAN020000192 DAZGAN020000000
VERSION     DAZGAN020000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.192"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(180..680)
                     /locus_tag="ACHKYO_RS23960"
                     /old_locus_tag="pgaptmp_004792"
     CDS             complement(180..680)
                     /locus_tag="ACHKYO_RS23960"
                     /old_locus_tag="pgaptmp_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGU4431480.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDETDPELMFQDYEGFPGNMASECHINWAWVEGFRRARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(1145..1741)
                     /locus_tag="ACHKYO_RS23965"
                     /old_locus_tag="pgaptmp_004793"
     CDS             complement(1145..1741)
                     /locus_tag="ACHKYO_RS23965"
                     /old_locus_tag="pgaptmp_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431481.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVSITDVLR"
     gene            complement(1738..2457)
                     /locus_tag="ACHKYO_RS23970"
                     /old_locus_tag="pgaptmp_004794"
     CDS             complement(1738..2457)
                     /locus_tag="ACHKYO_RS23970"
                     /old_locus_tag="pgaptmp_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HGU4431482.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            complement(2454..2888)
                     /gene="psiB"
                     /locus_tag="ACHKYO_RS23975"
                     /old_locus_tag="pgaptmp_004795"
     CDS             complement(2454..2888)
                     /gene="psiB"
                     /locus_tag="ACHKYO_RS23975"
                     /old_locus_tag="pgaptmp_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HGU4431483.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(2943..4901)
                     /locus_tag="ACHKYO_RS23980"
                     /old_locus_tag="pgaptmp_004796"
     CDS             complement(2943..4901)
                     /locus_tag="ACHKYO_RS23980"
                     /old_locus_tag="pgaptmp_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGU4431484.1"
                     /translation="MSVAEFKAKTGRKSSRKPSKEQETALSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAGSIQGVGLLQNLVVHTLPGERYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLAAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQDVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIDALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCTPRPQMDATEHTTNLADAA"
     gene            complement(4971..5204)
                     /locus_tag="ACHKYO_RS23985"
                     /old_locus_tag="pgaptmp_004797"
     CDS             complement(4971..5204)
                     /locus_tag="ACHKYO_RS23985"
                     /old_locus_tag="pgaptmp_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGU4431485.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEGGAGYWMNHVIRDFGILK"
     gene            complement(5262..5789)
                     /locus_tag="ACHKYO_RS23990"
                     /old_locus_tag="pgaptmp_004798"
     CDS             complement(5262..5789)
                     /locus_tag="ACHKYO_RS23990"
                     /old_locus_tag="pgaptmp_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4431486.1"
                     /translation="MSARGINKVIVVGRLGKDPEVCYIPNGGAVANLQVATSETWRDK
                     QTGEIREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
BASE COUNT         1289 a         1917 c         1638 g         1297 t
ORIGIN      
        1 gtccccgccc ccgggagtca gaacaacaga aacgacaaaa cagaaaaatg cctcacacca
       61 tgacgggcag gaagggcagc gccgcaggcg cggccctcct gtatttcagg caggcgtaag
      121 cctgccaggg ttttccggcg gcccggcagg ccataccccc gccggagcgg gggagtaaat
      181 caccgacgga acacatgacc gtcaataaag gtgaaggagt ccaggaataa atcccgcgca
      241 tacgcctcat agtcaaaata gagcgccacc gtctccggca cgtcagccag caggccggta
      301 tcactgacga actcaaccgc aaaagcctcc tcactgtcag cctcgcccca ccaggcatcg
      361 cggaagctgt caaaatccgt ctcaccggta tcatccaccc agagacgata agcctcttcg
      421 cagccttcat cccgtgcccg gcggaagcct tcaacccagg cccagttgat atgacattca
      481 gaggccatat tccccgggaa accctcataa tcctgaaaca tcagttccgg atcggtttca
      541 tcctggtgaa gggcacggca ggcggcgaaa aaatcgcgtt catcatcaaa cgtggtcagg
      601 tcaaaccagc ggccggcgat gcttccgcag ttatatttgt gccaggttcc aacgtataca
      661 gcaggtgcaa caacagacat tcctgaatcc tccatagtca catgttctgc gacaccgggc
      721 ggtgccgtgc ctccatcttt gccgggcctg cagggatgca agggcgcaag ggaagccgca
      781 gcccgacgcg cacggctgac gggagttgga gggtgagacc gcgaaatctg agcgggtgaa
      841 cgaccggaaa atcacggcag acgggaagga gggaccgggt gaccgcagcg gttcatctta
      901 ccttgcatcc cgcccgcaga agcccggcta cgatggaggc acggcacagc ccgcacacga
      961 cgctgaggcc acataactga aaacccacaa ggccatgccc cgcatggccg ggtaaaagcg
     1021 gagccgccgg cgacgtcctt attccggtga gagcagagga cacgccgcat gacctccaga
     1081 ccacccctga tatgaaacga tcatgaccgg catgtccctg cccctcaagc atacctccac
     1141 tggttcaccg aagaacatcc gtgatgctca caagttttct gtcgcgcagc cagtcctgcg
     1201 cttcacgcac cacacaactg tccggtttca ccgctttcag agaagatgcc agtcgcttga
     1261 tcaacatcac cagttcccgt gtctgtatgt ccggcattgt ctccggcgga gcagagggac
     1321 tctcatttcc gacgcccatc ccatgcgctt ccagtgtggc acggacaaac caggcagcat
     1381 cgaccagttg ctggcgaatc caggccagat cggcatttct gtcacgggct gccattcgct
     1441 cacccgacag cgccttgctg atatggctgc gcaccagatt atccaggagt tcctgcgctt
     1501 cttccggtga aaagccacct ggtatctctg tgcgcaacag tcctttcctg tacagtagct
     1561 gcagcagatc aatctgcgac cgcactgcaa ctatcagtac ataacggaca atattcgcac
     1621 tggcggtggt ttctgtatca ttcagcagtt cctccggatc attaccaccg gtattgcaga
     1681 gcaacggcaa cagaggtcgc atccacacgg gaacctgata ttgttcgtct gatttcatca
     1741 tattgcgttc tccagcttgt tcgggatcat ccactgctca agtgcacgta cctgtaccgg
     1801 tgcaccacga ccggcctcac cggcttcaaa aatcagccgc cagagtggtt cctcctgcca
     1861 gaaaaaccgg tcaaatgatg acagtgatgg aaaacgcccg caccaccccc agaaaatcgt
     1921 ttccagatca tgctcctcaa caacaccaga ccagcgggac aaccagcttc cgaccgtggc
     1981 gggagtatga aacgcaaggt caatctgcgc ctgagcaaaa catgtctggt attccagttc
     2041 ccgtcgttct gcctcacgtt cccgctggcg gctgtatttc tgctctgtca gcgtggtttt
     2101 ctgacgacga cgctcacctt cacgaaaagg aacggacggg aacagacgat gccccgcatc
     2161 ggatggcagg ggaagaacac agacttcacc aaaactttca atcagtttat ccacggcagc
     2221 cagccacagg agacggttgc catgaaattc ctccttcgtc agcgcagggg caaaaaagcg
     2281 gagatcggtc agcgaaatcc ggcgactgcc attcaggcag cgaaagaaag cgctggcata
     2341 aggccatgct gaaagtgtgc gtccctgacg acgacgttgt tcagtgacag ccacagcctg
     2401 catggcggcc tgttgttctg cgctgagggg gatcagtgcc cgtgaacgtg cgctcatgcc
     2461 tgacctcctt ctgccatcaa aatatgaatg atgtcgttaa cactgtatcc ctgcgcatca
     2521 agtgtcgcgg cgagcgccag cgtgtgactg atcgcgacag gcgtgaagat atgttgcacc
     2581 tgaacgacgg cgaaaggctg tccaccacca ttcaccagaa ccagcatcca gagttgagag
     2641 acgtctcccg gcgaacataa tgccaggtgg aaacgttcgt gggctggcgt gaaacgaacc
     2701 tggacgggga aaaagccccc gaactcactg ccatactcgc cattcatcat ccagttagcc
     2761 ggtgcgtcca cttcacgcat cacctcgtga gtgagattac ggcgcatatc gcttcccgca
     2821 gcacggatat cttcatattc ctgtgcgttc atggactgca gggcattcag ggtcagttca
     2881 gttttcatca cggtatctcc cttgctgccg ccccgtctcc ggcggggcgg tgtggttgtt
     2941 catcaggcgg catcagccag gttagtggtg tgttcagtgg catccatctg tggacgtggt
     3001 gtacacatcc agccaggcac ccagcggttg tctttcatat ggaactctgc atgttcagcc
     3061 gcatcgcctt tcttcatttt ctccgcgtcc cgcgcagcac cggacagtcc ggcatcattc
     3121 agggcatcga taatctgcgg ctttttcagg tgtccgaaaa agtttgcttt tgtcggctgc
     3181 caccagtcgc gcatgtggaa tccgatagcg ctctccagcg tgtccagcgg actgcgtgac
     3241 gtgtgaccac actcgcgggt ctgaacgccg tggatgctgc acgcggtgca gaaactgagc
     3301 agtgataaca gcacgtcctg gctgagtgac aggaacgtcg tcatatcccg tgaccatccc
     3361 tccggtaaca gggctgcaag acgggctttt tctgccatca tcgccatgaa tgcggcacct
     3421 tccttccccg atggcgcatc gctggtcagc gaataatgtt cacattccag gctgatttgt
     3481 gctggtttac tgtacgcccc gctgccaaac acattcaggc agagcgtcca tgccagcagt
     3541 gtcagggatt tgtccggttg ctgcatgagt gccgcctgga ctgccagcgt gcgttcggaa
     3601 gacattttcg tcagcagtgg cagactgatt tcctccactg acgctttctc ctgcacctgt
     3661 tccgtgcggt cagcgtcggc agccgcgtca tcctcgctgc gcaactgcac accacgctgg
     3721 acacacacgt tgccataacg ccaggacacc accacaccgc ttccggcacg catctccggc
     3781 gtccacgccc tgaccttcgc catgcagcgc atcagcttca tttctgctgc cagcaggtcg
     3841 gattcctcac actggttttc cagtgcgtcg taacgtgcca tcagttcgtt caggcgttct
     3901 tcttccgcct ccgtcagcac cgcttccggc tccggcagac agcggtatgt tccggcatcc
     3961 tcacggcact caccgactgg ctccatgcgc ccggcgcacc attcccagcc ttcggcttcc
     4021 cgaaggtgtt cagcaacagc ctggagtttt tccagcaggg cggcatcgag cgccacgcgg
     4081 tcgacatagc ccccctcgtc atcgctgaac aaatcggtgc gcagttcgtc tggcgagaag
     4141 gcatcagccc ccacgaagcg gaatttactg ttccccgcca ccgccacttc actttcggta
     4201 atcaggttgc ggataacccg cacatccggt ttaccgcccc atcctgactg gcaggcggct
     4261 tcaaacacct gcacctgacg cgcggtgtcg ttctccagcg ccagcgcctg acagtgttcg
     4321 gtggtgatgc ggtcttctgc cagcgcatcg aggatgacag gcgcaaggtc tgccagtttc
     4381 agcattcgct gaacgtggcg gggtgaatag cccagcaaat caccgatttg tgcaggtgtt
     4441 ttgccttcct gcgccattgc acggaatccg gcaatctgtt cggcagggtg catatcccga
     4501 cgatgaccgt tttcagtcat tgatgcggca gtcgccagct cctgcggaat aattttcacg
     4561 cggacaggcc agtcagccgg aatgattcca cgctctgcca gcatgttgag tgctgccagt
     4621 ctgcgaccac ctgcggcgac accgtagcgc tcaccaggca gggtatgcac gaccagattc
     4681 tgcaacagtc cgacgccctg aatggaccct gccagttcgc tgacggactc cgcagaatac
     4741 ggcacagtgc gcacattcag cggtgactta atcagtgaag ccagcggcac actcacttcc
     4801 tccgtctgcg ccagcagggc agacagggcg gtttcctgtt cttttgatgg tttacggctg
     4861 gatttacgac cagtttttgc cttaaactct gcaactgaca taaagatgct cctttacagg
     4921 tggttaacct tcgttatgtc tgccggacgc gttcccgtcc ggcacctctc ttacttaaga
     4981 atcccgaaat cacggatgac atggttcatc cagtacccgg caccgccctc aaaattccag
     5041 gtgcgccaga ccattgcacc gttattacgg acaaccaggc gaaactgttc gccgtggtca
     5101 tcctcgataa agacgttccg gtactgtgcg gcaacggctt cggcctgttt gcgggtaaaa
     5161 ggaccgtcag gcagtccctg aagtattctg aaatattcgc tcatcctcat ctctccggtg
     5221 atgccccgcg cagggcgggg cggttatcac attcaggcca gtcagaacgg gatatcgtct
     5281 gaaaacccgt aatcctcccc ctccggcggt tgcggctgag gctcgggctg cgcggcctta
     5341 cgtccacggc ctttcgtttt cgcgccacct tttttcgtcg cgtcagcact ctgtggctgc
     5401 ccattctgct gaggctgcgg ttgcgcctga gcgttctgct gtggtgcact ccccagcatc
     5461 tgcatggtgc ccgtggtttt aacaaggatt tcggtgacgt aacgggtgat accgttatcc
     5521 tcccagctac gggtgcgaag ctggccttcg atgtagacct gcgcgccctt gcgcagatat
     5581 tcacctgcca cttccgcgag cttgccgaac agcaccacgc ggtgccattc cgtctgctcc
     5641 cgtatctccc ccgtctgttt gtcgcgccag gtttctgacg tggccacctg caggtttgcc
     5701 actgcgcccc cgttggggat gtaacagact tccggatctt tacccagacg accaaccacg
     5761 atgaccttgt tgataccacg tgcactcata agaacctcct gactgaaaga tgtgagataa
     5821 accggatacc ggcgacggga cgttcatgtg ccccttcaga ggagcgaaag ctcctcctgc
     5881 cgtccacttc actggggttt ctccccgcag tggacctttc cgatgggggc ccgtttaccg
     5941 cctcacggga cagctgtccg ggggcggacg caaaattttt tgccgggttc atggctaaaa
     6001 attttgtggc cggtgcatct caccctggac agatggaacg ggagggggtt acgtctgccc
     6061 cccggaaagt ccacagcggg aaacaggccg cagaggtggc ggcaggaggg aggactgctg
     6121 cgccatcagg cgcagtttcc t
//
LOCUS       DAZGAN020000193         6047 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGAN020000193 DAZGAN020000000
VERSION     DAZGAN020000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.193"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..559
                     /locus_tag="ACHKYO_RS23995"
                     /old_locus_tag="pgaptmp_004799"
                     /pseudo
     CDS             1..559
                     /locus_tag="ACHKYO_RS23995"
                     /old_locus_tag="pgaptmp_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
     gene            565..1308
                     /locus_tag="ACHKYO_RS24000"
                     /old_locus_tag="pgaptmp_004800"
     CDS             565..1308
                     /locus_tag="ACHKYO_RS24000"
                     /old_locus_tag="pgaptmp_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4431487.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMAPEDWLRAEMQGEIVALVHSHPGALPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFV"
     gene            1245..1847
                     /locus_tag="ACHKYO_RS24005"
                     /old_locus_tag="pgaptmp_004801"
     CDS             1245..1847
                     /locus_tag="ACHKYO_RS24005"
                     /old_locus_tag="pgaptmp_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4431488.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPSFRQKLNE
                     GWYQVRIAGRDAGENELSARLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1908..5387
                     /locus_tag="ACHKYO_RS24010"
                     /old_locus_tag="pgaptmp_004802"
     CDS             1908..5387
                     /locus_tag="ACHKYO_RS24010"
                     /old_locus_tag="pgaptmp_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431489.1"
                     /translation="MGKGSSKGHTPREAKDNLKTTQLLSVIDAISEGPIEGPVEGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSLTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPRL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEVIVDNGAHFDGEQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIRPGHNYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNP
                     PAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKLSANQIEGDIVKTVG
                     KAFPRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLI
                     VKKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNDWHPTASISDLLV
                     VVMKKATAGISIS"
     gene            5455..>6047
                     /locus_tag="ACHKYO_RS24015"
                     /old_locus_tag="pgaptmp_004803"
     CDS             5455..>6047
                     /locus_tag="ACHKYO_RS24015"
                     /old_locus_tag="pgaptmp_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4431490.1"
                     /translation="MRKVYAAILSTAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSVYAMAGVAYSRVSTFFGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGY"
BASE COUNT         1359 a         1498 c         1903 g         1287 t
ORIGIN      
        1 gccggtcacc tagcaggggc gacagtatca gccgtatccc attcagggga gtggttttga
       61 actgaatggc aaaggcacca gtacgcgccc cacgctgacg gtttctaacc tgtacggtat
      121 ggtcaccggg atggcggaag atctgcagag tctggtcggc ggaacggtgg tccggcgtaa
      181 ggtttacgcc cgttttctgg atgcggtgaa cttcgtcaac ggaaacagcg acgccgatcc
      241 ggagcaggag gtgatcagcc gctggcgcat cgagcagtgc agcgaactga gcgcggtcag
      301 tgcttccttt gtgctctcca caccgacgga aacggatggt gccgtttttc cgggccgcat
      361 catgctggct aatacctgca cctggaccta tcgcggtgat gagtgcggtt atcacggtcc
      421 ggctgtcgcg gatgaatatg accagccgac gtccgatatc acgaaggata aatgcagcaa
      481 atgcctgagc ggttgtaagt ttcgcaataa cgtcggcaac tttggcggct tcctttccat
      541 taacaaactt tcgcagtaaa tcccatgaca gagacagaat cagcgattct ggcgcacgcc
      601 cggcgatgtg cgccagcgga gtcgtgcggc ttcgtggtaa gcacgccgga aggggaaaga
      661 tattttccct gcgtgaatat ctccggtgag ccggaggcgt atttccggat ggctccggag
      721 gactggctgc gggcagaaat gcagggtgag attgtggcgc tggtccacag tcaccccggt
      781 gctctgccct ggctgagtga ggccgaccgg cggctgcagg tgcagagtga tttgccgtgg
      841 tggctggtct gccggggggc gattcacaag ttccgctgtg tgccacatct taccgggcgg
      901 cgctttgagc acggggtgac ggactgttac acgctgttcc gggatgctta ccatctggcg
      961 ggaattgaga tgcctgattt tcatcgtgag gatgactggt ggcgtcacgg tcagaatctc
     1021 tatctggata atctggaggc cacagggctg tatcaggtgc cgttgtcagc ggcgcagccg
     1081 ggcgatgtgc tgctgtgctg ttttggttca tcggtgccga atcatgccgc catttactgc
     1141 ggcgacggcg agctgttgca ccatattcct gaacaactga gcaaacgaga gaggtacacc
     1201 gacaaatggc agcgacgcac acactccctc tggcgtcacc gggcatggca cgcatctgcc
     1261 tttacgggga tttacaacga tttggccgcc gcatcgacct tcgtgtgaaa acgggggccg
     1321 aagccatccg ggcgctggcc acgcagcttc cgtcgtttcg ccagaaactg aatgagggct
     1381 ggtatcaggt gcgcattgcc gggcgtgatg caggcgaaaa tgaattatca gcccgtctta
     1441 atgaaccgct ggcaaatggt gctgtgatcc atatcgtgcc gcgtctggca ggagccaaaa
     1501 gtggcggtgt gtttcaggct gtgctggggg cggctgttat ggcggttgct atatggatgc
     1561 cgggggtagg aattatggcg agtaatctgc tgttttctct cggtgccagt atggtgctcg
     1621 gtggtgtggc gcagatgctg gcaccgaaag ccagaactcc ccgcacacag acaacggata
     1681 acggcaaaca gaacacctat ttttcttcac tggataatat ggttgcccag ggcaatgtcc
     1741 tgcccgttct gtacggtgaa atgcgtgtgg ggtcgcgggt ggtatctcag gagatcagca
     1801 cagcagatga aggggatggt ggtcaggttg tggtgattgg tcgctgatgc aaaatgtttt
     1861 atgtgaaacc gcctgcgggc ggttttgtcg tttatggagc gtgaggaatg ggtaaaggaa
     1921 gcagtaaggg gcatactccg cgcgaagcga aggacaacct gaagaccacg cagttgctga
     1981 gtgtgatcga tgccatcagc gaagggccga ttgaaggtcc ggtggaggga ttaaaaagcg
     2041 tgctgctgaa cagtacgccg gtgctggaca gtgaggggaa taccaatatc tccggcgtca
     2101 cggtggtgtt ccgggccggt gagcaggagc agactccgcc ggagggattt gaatcctccg
     2161 gctccgagac ggtgttgggt acggaagtga aatacgacac gccgatcacc cgcgccatca
     2221 cgtcggcaaa catcgaccga ctgcgcttta ccttcggtgt gcaggcactg gtggaaacca
     2281 cctcaaaggg ggaccggaat ccgtcggaag ttcgcctgct ggttcagata cagcgtaatg
     2341 gtggctgggt gacggaaaaa gacatcacca ttaagggcaa aaccacctcg cagtatctgg
     2401 cctcggtggt ggtggataac ctgccgccgc gcccgtttaa tatccggatg cgcagaatga
     2461 cgccggacag taccacagac cagctgcaga acaaaacgct ctggtcgtca tacaccgaaa
     2521 tcatcgatgt gaaacagtgc tacccgaaca cggcactggt cggcgtacag gtggattcgg
     2581 agcagttcgg cagccagcag gtgagccgta attatcatct gcgcgggcgt attctgcagg
     2641 tgccgtcgaa ttataacccg cagacgcggc aatacagcgg tatctgggac ggaacgttta
     2701 aaccggcata cagcaacaac atggcctggt gtctgtggga tatgctgacc cacccgcgct
     2761 acggcatggg gaaacgtctt ggtgcggcag atgtggataa atgggcgctg tatgtcatcg
     2821 gccagtactg cgaccagtcg gtgccggacg gctttggcag cacggagccg cgcatcacct
     2881 gtaatgcctg gctgaccaca cagcgcaagg cgtgggatgt gctcagtgat ttctgctcgg
     2941 cgatgcgctg tatgccggta tggaacgggc agacgctgac gttcgtgcag gaccgaccgt
     3001 cggataaggt gtggacctat aaccgcagta atgtggtgat gccggatgat ggcgcgccgt
     3061 tccgctacag cttcagcgcc ctgaaggacc gccataatgc cgttgaggtg aactggattg
     3121 acccggataa cggctgggag acggcgacag agcttgtgga ggacacgcag gccattgccc
     3181 gttacggtcg taatgtcacg aagatggatg cctttggctg taccagccgg gggcaggcac
     3241 accgagccgg gctgtggctg attaaaacgg agctgctgga aacgcagacc gtggatttca
     3301 gcgtcggcgc agaagggctt cgccatgtac cgggcgatgt tattgaaatc tgtgatgatg
     3361 actatgccgg tatcagcacc ggtggtcgtg tgctggcggt gaacagcctg acccggacgc
     3421 tgacgcttga ccgtgaaatc acgctgccat cctccggtac cacgctgata agcctggttg
     3481 acggaagtgg caatccggtc agcgtggagg ttcagtccgt caccgacggc gtgaaggtga
     3541 aagtgagccg tgttcctgac ggtgttgctg aatacagcgt atgggggctg aagctgccga
     3601 ggctgcgcca gcgactgttc cgctgcgtga gtatccgtga gaacgacgac ggcacgtatg
     3661 ccatcaccgc cgtgcagcat gtgccggaaa aagaggtcat cgtggataac ggggcgcact
     3721 ttgacggcga acagagtggc acggtgaacg gtgtcacgcc gccagcggtg cagcacctga
     3781 ccgcagaagt cactgcagac agcggggaat accaggtgct ggcccgctgg gacacgccga
     3841 aggtggtgaa gggcgtgagc ttcctgcttc gcctgaccgt ggcagcggat gacggcagtg
     3901 agcggctggt cagcacggcc cggacgacgg aaaccactta ccgcttcaca caactggctc
     3961 tggggaacta caggctgaca gtccgggcgg taaatgcgtg ggggcagcag ggcgatccgg
     4021 cgtcggtatc gttccggatt gccgcaccgg cagcgccgtc gcggattgag ctgacgccgg
     4081 gctattttca gataaccgcc acgccgcatc ttgcggttta tgatccgacg gtacagtttg
     4141 agttctggtt ctcggaaaag cggattgcgg atatcaggca ggttgaaacc acagcacgct
     4201 atcttggcac ggcgctgtac tggatagccg ccagtatcaa tatcaggccg ggccataatt
     4261 attattttta cgttcgcagt gtgaacaccg ttggcaaatc ggcattcgtg gaggctgttg
     4321 gtcagccgag tgatgacgca tccggctatc tggatttttt caaaggcgag atagggaaaa
     4381 cccatctggc tcaggagctg tggacgcaga ttgataacgg tcagcttgcg cctgacctgg
     4441 ctgaaatcag gacatccatt acggatgtca gcaatgaaat cacacagacc gtcaataaga
     4501 aactggaaga ccagagtgca gcgatccagc agatacagaa ggttcaggtt gatacaaata
     4561 ataatctgaa cagcatgtgg gctgtgaagc tgcagcagat gcaggacgga cgcctttata
     4621 ttgcgggtat cggtgccggt attgagaaca cccccgacgg catgcagagt caggtactgc
     4681 tggcggcaga caggattgcg atgattaatc ctgcaaatgg caacacaaaa ccgatgtttg
     4741 ttggtcaggg tgatcagata ttcatgaatg aagtgttcct gaaatatctg acggctccca
     4801 ccattaccag cggcggtaat cctcctgcat tttccctgac accggacgga aagctgaccg
     4861 ctaaaaatgc agatatcagt ggcagtgtga atgcgaacgc cgggacgctc aacaacgtta
     4921 cgataaatga aaactgtcag attaaaggga aactgtcagc caaccagatt gaaggcgata
     4981 ttgtcaaaac agtgggtaag gctttcccgc gggactcccg ggcaccggag cggtggccat
     5041 cagggaccat taccgtcagg atttatgacg atcagccgtt tgaccggcag attgttattc
     5101 cggcggtggc attcagcggt gctaaacatg agagagagca tactgatatt tactcctcat
     5161 gccgcctgat agtgaagaaa aatggtgctg aaatttataa ccgtaccgcg ctggataata
     5221 cgctgattta cagtggtgtt attgatatgc cagctggtca cggccacatg acgctggagt
     5281 tttcggtgtc agcatggctg gtaaatgact ggcatcccac agcaagtatc agcgatttgc
     5341 tggttgtggt gatgaagaaa gccaccgcag gcatcagtat cagctgaatt ttataaccca
     5401 tatacgggcg ccagaaatgg cgcctttttt attgcagaaa agcgagaggt aattatgcgt
     5461 aaagtttatg cagccatttt gtccacagcc atctgtctgg ccgtatccgg tgcgcctgca
     5521 tgggcgtctg agcagcaggc cacactgagc gcagggtatc ttcatgcccg tacgaacgct
     5581 cccggcagcg ataatctgaa cgggattaac gtgaaatacc gttatgagtt tacggacaca
     5641 ctggggctga ttacgtcatt cagttatgcc aacgctgaag atgagcaaaa aacgcattac
     5701 agcgataccc gctggcatga agattccgtg cgtaaccgct ggttcagcgt aatggcgggg
     5761 ccgtctgtgc gcgtgaatga atggttcagc gtgtatgcga tggcgggtgt ggcttacagc
     5821 cgtgtgtcga ctttcttcgg ggattatctc cgcgtaactg acaacaaggg gaaaacgcac
     5881 gatgtgctga ccggaagtga tgacggtcgc cacagcaaca cgtctctggc gtggggggct
     5941 ggcgtgcagt ttaacccgac cgaatccgtg gccattgata ttgcttatga aggctccggc
     6001 agtggtgact ggcgcactga cggtttcatc gtgggtgtcg gttataa
//
LOCUS       DAZGAN020000194         5994 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGAN020000194 DAZGAN020000000
VERSION     DAZGAN020000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.194"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHKYO_RS24020"
                     /old_locus_tag="pgaptmp_004804"
     CDS             complement(<1..593)
                     /locus_tag="ACHKYO_RS24020"
                     /old_locus_tag="pgaptmp_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4431491.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGY"
     gene            complement(660..4058)
                     /locus_tag="ACHKYO_RS24025"
                     /old_locus_tag="pgaptmp_004805"
     CDS             complement(660..4058)
                     /locus_tag="ACHKYO_RS24025"
                     /old_locus_tag="pgaptmp_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431492.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDNEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRMLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     AIQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSELLVSTARTAETTYRFRQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            complement(4119..4691)
                     /locus_tag="ACHKYO_RS24030"
                     /old_locus_tag="pgaptmp_004806"
     CDS             complement(4119..4691)
                     /locus_tag="ACHKYO_RS24030"
                     /old_locus_tag="pgaptmp_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4431493.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLTAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPTATSTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(4688..5431)
                     /locus_tag="ACHKYO_RS24035"
                     /old_locus_tag="pgaptmp_004807"
     CDS             complement(4688..5431)
                     /locus_tag="ACHKYO_RS24035"
                     /old_locus_tag="pgaptmp_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4431494.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRSWRASAFTGICNDLAAASTFV"
     gene            complement(5436..>5994)
                     /locus_tag="ACHKYO_RS24040"
                     /old_locus_tag="pgaptmp_004808"
     CDS             complement(5436..>5994)
                     /locus_tag="ACHKYO_RS24040"
                     /old_locus_tag="pgaptmp_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4431495.1"
                     /translation="PVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGM
                     AEDLQSLVGGTVVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSAS
                     FVLSTPTETDGAVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSK
                     CLSGCKFRNNVGNFGGFLSINKLSQ"
BASE COUNT         1260 a         1866 c         1513 g         1355 t
ORIGIN      
        1 ttataaccga cacccacgat gaaaccgtca gtgcgccagt cgccactgcc ggaaccttca
       61 taagcaatat caatggccac ggattcggtc gggttaaact gcacgccagc tccccacgcc
      121 agagacgtgt tgctgtggcg accgtcatca cttccggtca gcacatcgtg cgttttcccc
      181 ttgttgtcag ttacgcgaag ataatccccg gagaaagtcg acacacggct gtaagccaca
      241 cccgccatcg catacgcgct gaaccattca ttcacgcgca cagacggccc cgccatcacg
      301 ctgaaccagc ggttacgcac ggaatcttca tgccagcggg tatcgctgta atgggtcagc
      361 tggcgattct tgtctcctgc atagctgaat gacgtcacca tccccagtgt gtccgtaaac
      421 tcataacggt atttcacgtt aatcccgttc agatcatcgc tgccgggaac gttcgtcgag
      481 gcatgaagat accccgcgct cagcgtggac tgatgttcag acgcccatgc aggcgcaccg
      541 gatacggcca gacaaatggc tgcggacaaa atggcggcat aaagtttacg cataattacc
      601 tctcgctttt ctgcaataaa aaaggcgtca tttctgacgc ccgttatggg ttataaaatt
      661 cagctgatac tgatacctgc tgtggatttc ttcatcacca caaccagcag atcgctgata
      721 ctggttgttg gtgtccagtt attcgctcct gatgaagata cggtgaatgt cagtgtcagt
      781 gtcccctgtc cggcaggcat atctataact gaggaaaata cgccctgagc atccgtcgtg
      841 gactgattaa aaatctcctg accattgcgg gtcactctta accggcaggt tgaataccag
      901 tatgactgtt ggttattact gttgaaattc tcatgcttac caccgcggaa taacactggc
      961 ggtatcatga cctgccggtc aaatttctga tcatcactga ttcttaccgt gatggtgcca
     1021 ctggcataac tgttcgtgcg ggggaaagac ttgctgaccg ttttgacaat atcaccttca
     1081 atctggttgg ctgacagttt ccccttaatc tgacagttct cattaatcgt gacgttgttg
     1141 agcgtccctg agttcgcatt cacactgcca ctgatatccg catttttagc ggtcagcttt
     1201 ccgtctgatg tcagggaaaa taccggagga ctgccaccgc tggtaatggt gggtgccgtc
     1261 aggcgtttca ggaacacgtc gttcatgaat atctggttgc cctgcgccac aaacatcggc
     1321 gtttcattcc cgtttgccgg gtcaataaac gcgatacggt tagcggcaac cagaaactgg
     1381 ctcagcttgc cttcctccgt gtcctccata ctgaggccaa tacccgcgac ataatgtttg
     1441 ccgtctttgg tctgctcaat tttgacagcc cacatggcat tccatttatc gttggcatcc
     1501 ttccactctt tcgaaaactc atccagtctg ctggcgttat cctccgtcag atctactttt
     1561 tccagcagct ctttaccgag atgggattcg gttatcttgc ctttgaaaaa atccaggtaa
     1621 ccttccgcat catcgctcgc ccgaccgacg gcctccacga atgccgattt gccaacggtg
     1681 ttcacactgc ggatataaaa gtaataatca tggcccggtt tgatattgat actggcggct
     1741 atccagtaca gccccgtgcc aaggtagcgg gctgtggttt caacctgcct gatatcggta
     1801 atccgcgttt ccgagaacca gaactcaaac tgtaccgtcg gatcataaac cgcaagatgc
     1861 ggcgtggcag ttatctgaaa atagcccggc gtcagctcaa tctgtgacgg cgctgccggt
     1921 gcggcaatcc ggaacgatac cgatgccgga tcgccctgct gtccccacgc atttaccgcc
     1981 cggactgtca gcctgtagtt ccccagcgcc agttgcctga agcggtatgt ggtttccgcc
     2041 gtccgggccg tgctgaccag cagctcactg ccgtcatccg ctgccacggt caggcgaagc
     2101 aggaaactca cgcccttcac caccttcggc gtgtcccagc gcgccagcac ctgatattcc
     2161 ccgctgtctg cggtgacttc ggcggtcagg tgctgcaccg ctggcggcgt gacaccattc
     2221 accgtgccgc tctggtcgcc gtcaaagtgc gccccgttat ccacgatggc ctctttttcc
     2281 ggtacatgct gcacggcggt gatggcatac gtgccgtcgt cgttctcacg gatactcacg
     2341 cagcggaaca ggcgctggcg cagcgtcggc agcttcagcc cccacacgct gtattcagca
     2401 acgccgtcag gaacacggct cactttcacc ttcacgccgt cggtgacgga ctgaacctcc
     2461 acgctgaccg gattgccact tccgtcaacc aggcttatca gcgtggtacc ggaggatggc
     2521 agagtgattt cacggtcgag cgtcagcgtc cgggtctggc tgtttaccgc cagcacgcgc
     2581 ccgccgatgc tgataccggc atagtcatca tcacagattt caatgacatc gcccggcaca
     2641 tggcgaagcc cttctgcgcc cacgctgaag tccacggtct gcgtttccag cagttctgtt
     2701 ttaatcagcc acagcccggc gcggtgtgcc tgcccccggc tggtacagcc aaaagcatcc
     2761 atcttcgtga cgttacgacc gtaacgggca atggcctgcg tatcctccac aagctctgtt
     2821 gccgtctccc agccgttgtt cgggtcaatc cagttcacct caacggcatt atggcggtct
     2881 ttcagggcgc tgaagctgta gcggaacggc gcgccatcat ccggcatcac cacattactg
     2941 cggttatagg tccacacctt atccgacggt cggtcctgca cgaacgtcag cgtctgcccg
     3001 ttccataccg gcatacagcg catcgccgag cagaaatcac tgagcacatc ccacgccttg
     3061 cgctgtgtgg tcaggtacgc attacaggtg atgcgcggct ccgtgccacc aaagccgtcc
     3121 ggcactgact ggtcgcagta ctggccgatg acatacagcg cccatttgtc cacatccgcc
     3181 gcaccaagac gtttccccat gccgtagcgc gggtgggtca gcatatccca cagacaccag
     3241 gccatgttgt tgctgtatgc tggttttaac gttccgtccc agataccact gtattgtcgc
     3301 gtctgcgggt tatagttcga cggcacctgc agaatgcgcc cgcgaagatg ataattacga
     3361 ctcacctgct ggctgccgaa ctgctccgag tccacctgta tggcgaccag tgcagtgttc
     3421 gggtagcact gtttcacatc gatgatttcg gtgtatgacg accagagcgt tttgttctgc
     3481 agctggtctg tagtactgtc cggtgtcatc ctgcgcatcc ggatattgaa cgggcgcgga
     3541 ggcaggttat ccaccaccac cgaggccaga tactgcgagg tggttttgcc cttaatggtg
     3601 atgtcttttt ccgtcaccca gccaccgtta cgctgtatct gaaccagcat gcggacttcc
     3661 gacggattcc tgtctccctt tgaggtggtt tccaccagtg cctgtacacc gaaggtaaag
     3721 cgcagacggt cgatgtttgc cgacgtgatg gtccgggtga tcggcgtgtc atatttcact
     3781 tccgtaccca gcacagtctc ggagccggag gattcaaacc cctccggcgg tgactgctcc
     3841 tgctcacctg cccggaacac caccgtgacg ccggagatgt tggtattccc ctcattgtcc
     3901 agcaccggcg tactgttcag cagcacgctt tttaatccat ccaccggacc ttcaaccggc
     3961 ccttcactga tggcatcgat cacactcagc agttgcgtgg acttcaggtt gtccttcgct
     4021 tcgcgcgggg tatgcccctt actgctgcct ttacccattc ctcacgctcc ataaacgaca
     4081 aaacctcccg caggcggttt cacataaaac attttgcatc agcgaccaat caccacaacc
     4141 tgaccaccat ccccttcgtc tgccgtgctg atctcctgag aaaccacgcg tgaccccacg
     4201 cgcatttcac cgtacagaac aggcagaaca ttgccctggg caaccatgtt atccagtgat
     4261 gaaaaatagg tgttctgctt accgttatcc gtgctggtcg ctgtgggcgt ccgggctttc
     4321 ggtgccagca tctgcgccac accaccgagc accatactgg caccgagaga aaacaggatg
     4381 ccggtcatac caccggcccc aatggctgcc ccccatgctg taagggtggc tccggcggta
     4441 aagaatgatc cggcaatggc ggctgctccc aggacaatct ggaatacacc acctgacttg
     4501 gccccggcga ctctgggaac aatatgaatc acagcgccat caggcagagt ctcatgtaac
     4561 tgcgccgtta atccggacgt gctgacatcc tgcccggcaa tccgtacctg ataccagccg
     4621 tcgctcagtt tctgacgaaa cgccgggagc tgtgtggcca gtgcgcggat ggcttcagcc
     4681 cccgttttca cacgaaggtc gatgcggcgg ccaaatcgtt gcaaatcccc gtaaaggcag
     4741 atgcgcgcca tgaccggtga cgccagaggg agtgtgtgcg tcgctgccat ttgtcggtat
     4801 acctctctcg tttgctcagt tgttcaggaa tatggtgcag cagctcgcca tcaccacagt
     4861 aaatggcggc atgattcggc accgatgaac caaaacagca cagcagcaca tcgcccggtt
     4921 gtgctgatga caacggcacc tgatacagcc ctgtggcctc cagattatcc agatagagat
     4981 tctgaccgtg acgccaccag tcatccccgc gatgaaaatc cggcatctca atccccgcca
     5041 gatgataagc atcccggaac agcgtgtaac agtccgtcac cccgtgctca aagcgccgcc
     5101 cggtgagatg cggcacacag cggaacttgt gaatcgcccc ccggcagacc agccaccacg
     5161 gcaaatcact ctgcacctgc agccgccggt cagcctcact cagccagggc agaccaccgg
     5221 gatgactgtg gaccagtgcc acaatctcac cctgcatctc tgcctgcagc cagtcctccg
     5281 gagccatccg gaaataatcc tccggctcac cggaaatatt cacgcaggga aaatatcttt
     5341 ccccctccgg cgctctcacc acgaagccgc acgactccgc tggcgcacat cgccgggcgt
     5401 gcgccagaat cgctgattct gtctctgtca tgggattact gcgaaagttt gttaatggaa
     5461 aggaagccgc caaagttgcc gacgttattg cgaaacttac agccgctcag gcatttgctg
     5521 catttatcct tcgtgatatc ggacgtcggc tggtcatatt catccgcaac tgccggaccg
     5581 tgataaccgc actcatcacc gcgataggtc caggtgcagg tgttggccag catgatacgt
     5641 cccggaaaaa cagcgccatc cgtttccgtc ggcgtggaca gtacaaagga ggcactcacc
     5701 gcgctcagtt cgctgcactg ctcgatgcgc cagcggctga tcacctcctg ctccggatcg
     5761 gcgtcactgt ttccgttgac gaagttcacc gcatccagaa aacgggcgta aaccttacgc
     5821 cggaccaccg ttccgccgac cagactctgc agatcttccg ccatcccggt gaccataccg
     5881 tacaggttag aaaccgtcag cgtggggcgc gtactggtgc ctttgccatt cagttcaaaa
     5941 ccactcccct gaatgggata cggctgatac tgtcgcccct gccaggtgac cggc
//
LOCUS       DAZGAN020000195         5976 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGAN020000195 DAZGAN020000000
VERSION     DAZGAN020000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.195"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            126..1277
                     /gene="yiaY"
                     /locus_tag="ACHKYO_RS24045"
                     /old_locus_tag="pgaptmp_004809"
     CDS             126..1277
                     /gene="yiaY"
                     /locus_tag="ACHKYO_RS24045"
                     /old_locus_tag="pgaptmp_004809"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HGU4431496.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1442..2980
                     /gene="aldB"
                     /locus_tag="ACHKYO_RS24050"
                     /old_locus_tag="pgaptmp_004810"
     CDS             1442..2980
                     /gene="aldB"
                     /locus_tag="ACHKYO_RS24050"
                     /old_locus_tag="pgaptmp_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HGU4431497.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3199..3354)
                     /locus_tag="ACHKYO_RS24055"
                     /old_locus_tag="pgaptmp_004811"
     CDS             complement(3199..3354)
                     /locus_tag="ACHKYO_RS24055"
                     /old_locus_tag="pgaptmp_004811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431498.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLIRP
                     LTIETRD"
     gene            3525..3848
                     /gene="yiaW"
                     /locus_tag="ACHKYO_RS24060"
                     /old_locus_tag="pgaptmp_004812"
     CDS             3525..3848
                     /gene="yiaW"
                     /locus_tag="ACHKYO_RS24060"
                     /old_locus_tag="pgaptmp_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU4431499.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3854..4990
                     /gene="yiaV"
                     /locus_tag="ACHKYO_RS24065"
                     /old_locus_tag="pgaptmp_004813"
     CDS             3854..4990
                     /gene="yiaV"
                     /locus_tag="ACHKYO_RS24065"
                     /old_locus_tag="pgaptmp_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4431500.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4987..5961)
                     /gene="yiaU"
                     /locus_tag="ACHKYO_RS24070"
                     /old_locus_tag="pgaptmp_004814"
     CDS             complement(4987..5961)
                     /gene="yiaU"
                     /locus_tag="ACHKYO_RS24070"
                     /old_locus_tag="pgaptmp_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4431501.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1483 a         1422 c         1548 g         1523 t
ORIGIN      
        1 attgtttaat gaatacaatg tgctttttat tagattaatt ttgataatca ataataatga
       61 tcatgttttc gaatgaaaat cctcagtaag ctgcccaccc ttttttacac tttcaggagt
      121 gtgttatggc atcttcaact ttctttattc cttctgtgaa tgtcatcggc gctgattcat
      181 tgactgatgc aatgaatatg atggcagatt atggatttac ccgtacctta attgtcactg
      241 acagtatgtt aacgaaatta ggtatggcgg gcgatgtgca aaaagcactg gaagaacgca
      301 atatttttag cgttatttat gatggcaccc aacctaaccc caccacggaa aacgtcgccg
      361 caggtttgaa attacttaaa gagaataatt gcgatagcgt gatctcctta ggcggtggtt
      421 ctccacacga ctgcgcaaaa ggtattgcgc tggtggcagc caatggcggc gatattcgcg
      481 attacgaagg cgttgaccgc tctgcaaaac cgcagctgcc gatgatcgcc atcaatacca
      541 cggcgggtac ggcctctgaa atgacccgtt tctgcatcat cactgacgaa gcgcgtcata
      601 tcaaaatggc gattgttgat aaacatgtca ctccgctgct ttctgtcaat gactcctctc
      661 tgatgattgg tatgccgaag tcactgaccg ccgcaacggg tatggatgcc ttaacgcacg
      721 ctatcgaagc gtatgtttct attgccgcca cgccgatcac tgacgcttgt gcactgaaag
      781 ccgtaaccat gattgccgaa aacctgccgt tagccgttga agatggcagt aatgcgaaag
      841 cgcgtgaagc aatggcttat gcccagttcc tcgccggtat ggcgttcaat aatgcttctc
      901 tgggttatgt tcatgcgatg gcgcaccagc tgggcggttt ctacaacctg ccacacggtg
      961 tatgtaacgc cgttttgctg ccgcatgttc aggtattcaa cagcaaagtc gccgccgcac
     1021 gtctgcgtga ctgtgccgct gcaatgggcg tgaacgtgac aggtaaaaac gatgcggaag
     1081 gtgctgaagc ctgcattaac gccatccgtg aactggcgaa gaaagtggat atcccggcag
     1141 gcctacgcga cctgaacgtg aaagaagaag atttcgcggt tctggcgact aatgccctga
     1201 aagatgcctg tggttttact aacccgatcc aggcaactca cgaagaaatt gtggcgattt
     1261 atcgcgcagc gatgtaatca tcatttccac aacggctggc aaattgtcag ccgctttttc
     1321 aactatctct gtaacccttg cccgtaaatt cgtgatagct gtcgtaaagc tgttaccgac
     1381 tggcgaagat ttcgccagtc acgtctaccc ttgttatacc tcacaccgca aggagacgat
     1441 catgaccaat aatccccctt cagcacagat taagcccggc gagtatggtt tccccctcaa
     1501 gttaaaagcc cgctatgaca actttattgg cggcgaatgg gtagcccctg ccgacggcga
     1561 gtattaccag aatctgacgc cggtgaccgg gcagctgctg tgcgaagtgg cgtcttcggg
     1621 caaacgagac atcgatctgg cgctggatgc tgcgcacaaa gtgaaagata aatgggcgca
     1681 cacctcggtg caggatcgcg cggcgattct gtttaagatt gccgatcgaa tggaacaaaa
     1741 cctcgagctg ttagcgacag ctgaaacctg ggataacggc aaacccattc gcgaaaccag
     1801 tgctgcggat gtaccgctgg cgattgacca tttccgctat ttcgcctcgt gtattcgggc
     1861 gcaggaaggt gggatcagtg aagttgatag cgaaaccgtg gcctatcatt tccatgaacc
     1921 gttaggcgtg gtggggcaga ttatcccgtg gaacttcccg ctgctgatgg cgagctggaa
     1981 aatggctccc gcgctggcgg cgggcaactg tgtggtgctg aaacccgcac gtcttacccc
     2041 gctttctgta ctgctgctaa tggaaatcgt cggtgattta ctgccgccgg gcgtggtgaa
     2101 cgtggtcaac ggcgcaggtg gggaaattgg cgaatatctg gcgacctcga aacgcatcgc
     2161 caaagtggcg tttaccggct caacggaagt gggccaacaa attatgcaat acgccacgca
     2221 aaacattatt ccggtgacgc tggagctggg cggcaaatcg ccaaatatct tctttgctga
     2281 tgtgatggat gaagaagatg cctttttcga taaagcgctg gaaggctttg cactgtttgc
     2341 ctttaaccag ggtgaagttt gcacctgtcc gagtcgtgcc ttagtgcagg agtcaatcta
     2401 cgaacgcttt atggaacgcg ccatccgccg tgtcgaaagc attcgcagcg gtaacccact
     2461 cgacagcgtg acgcaaatgg gcgcgcaggt ttctcacggg caactggaaa ccatcctcaa
     2521 ctacattgat atcggtaaaa aagagggcgc agacgtgctc accggcgggc ggcgcaagct
     2581 gctggaaggt gaactgaaag acggctacta cctcgaaccg acgattctgt ttggtcagaa
     2641 caatatgcgg gtgttccagg aggagatttt tggcccggtg ctggcggtga ccaccttcaa
     2701 aacgatggaa gaagcgctgg agctggcgaa cgatacgcaa tatggcctgg gcgcaggcgt
     2761 ctggagtcgt aacggtaatc tggcctataa gatggggcgc ggcattcagg ccgggcgcgt
     2821 gtggaccaac tgctatcacg cttacccggc acatgctgcg tttggtggct acaaacaatc
     2881 aggtatcggt cgcgaaaccc acaagatgat gctggagcat taccagcaaa ccaagtgcct
     2941 gctggtgagc tactcggata aaccgttggg gctgttctga tataagaagc tggtcgcatt
     3001 gggtattcat tgcctgatgc gacgcttacg cgtcttatca tgcctacggg aacctgaccg
     3061 taggccggat aaggcgtaca cgccgcatcc ggcataaaca aagcgcacgt tgttacaatt
     3121 tacgttttaa acctacgtgc gcaataaccc agactacgcc aacagaagtg cgtttctggg
     3181 gcgtgttttg tcaggagttt aatctcgcgt ttcgatggtg agcgggcgaa tcaggccgta
     3241 aatcaccatt ggtgatccga ggccaatcag caataaacgg tagataattg aaggtttaat
     3301 acgcttaatc tgtactgtgg atgttttact ggagttattc attagtttgg ccaatagttc
     3361 cattatttca atatgataag tctcgacctg ttgaaaagcc aggtagcaca cttcagaaag
     3421 tatataaaaa acaatatgtc gttatataaa taaggaaatt gcactgcatc atggcatttc
     3481 ataatatgcg ttaattgttc ttttttatat tctctgaggg aaatatgttc ctggactatt
     3541 ttgcactggg agtgcttatt tttgtatttc tggtgatctt ctatgggatt attattttac
     3601 atgatattcc ctacctgatt gctaaaaaac gtaatcatcc tcatgccgac gccattcatg
     3661 ttgctggttg ggtgagtctt tttacgttac atgttatctg gccatttttg tggatttggg
     3721 ccacgcttta ccgcccggag cggggatggg gaatgcaaaa ccatgactcc tccgttgtgc
     3781 agctacaaca gcgtattgcc gggctggaga aacagctcgc cgacatcaaa tcctcttctg
     3841 ccgagtaata cttatggatt tactgattat tttgacctat gtggctttcg catgggcaat
     3901 gtttaagatc ttcaaaattc ctgtaaataa atggaccatt cccacagcgg ccctgggtgg
     3961 catatttatt gtcagtggtt taattctgtt aatgaactat aaccatccgt ataccttcaa
     4021 agcgcaaaaa gcggttattt ctattcctgt tgtcccacag gtgacaggcg tggtgatcga
     4081 agtgacggat aagaaaaata cgctgattaa aaaaggtgag gtgctatttc gactggaccc
     4141 gacgcgttat caggcgcggg tggatcggct gatggcggat atcgttaccg cagaacataa
     4201 acagcgggcg ttgggcgcag agttagatga gatggcggcg aatactcagc aggcaaaggc
     4261 cacgcgggat aaattcgcta aagagtatca gcgttacgca cgcggcagcc aggcgaaagt
     4321 aaacccgttt tcagaacgcg atattgatgt ggcgcggcaa aattatctgg cgcaggaagc
     4381 ctccgtaaag tcatcggcgg cggaacaaaa acagatccag agccagctgg atagcctggt
     4441 gttgggtgaa cattctcaaa tcgccagcct gaaagcgcag ctcgcggaag caaaatataa
     4501 ccttgagcag acgatagtgc gtgcgccgag cgatggttat gtgacccagg tgctgattcg
     4561 tccgggtacc tatgccgcgt cgctgccgct acgtccggtg atggtgttta tacccgatca
     4621 gaaacgacaa attgtggcgc agttccgtca gaactccttg ctgcgactgg cccctggcga
     4681 tgatgcggaa gtggtattta acgctctgcc aggtaaggta ttcagcggca agctggcagc
     4741 cattagtcca gctgttcccg gcggagctta tcagtcgacc ggcaccttac agacgttaaa
     4801 cacagcgccg ggttcagatg gcgttatcgc gaccattgaa ctggatgagc acactgattt
     4861 gagcgcttta ccagacggta tttacgccca ggtggcggtc tactctgatc atttcagcca
     4921 tgtctcggtg atgcgcaaag tgctgttacg catgaccagt tgggtgcatt acctttatct
     4981 cgatcattaa tcttcgcttt ccgccgcttg ttcaggccgc agttgctgcg actgagcaag
     5041 ctggtggcga atttcacgca gcatttcatc cagcacatac tggacccgcc agggctggta
     5101 ttcccgttta cggaagatag ccaccagatc cagccaccat tcatacgggt gctgaaagca
     5161 gggcaccagc gtgccgtctt gcaggtcgct ctgcacactt ttgtctggcg caaacacaat
     5221 ccccaggtga tttcgtgcca gctccagcgc cgattgcgtg ttatcgcaaa cgtaatttcc
     5281 cttcacgcga taatcccgca cctctttact cccggcgacg ttgaagcgcc agatattcgc
     5341 gtcgtcaatc atcatcgagt cgatcaaaat acaggagtga tgttcgagtt cgtcaggacg
     5401 gctaatagga tgtttctcaa gatagcgctg gctggcataa gcggttacgg catattgggt
     5461 aataaaactg gcaaccagcg attcatcttt tggtggagcg tagctgatta aaacatcaca
     5521 atcatcagga aattcgacgc cttcataaaa ggcattacgc tcaagattgc aggtttttag
     5581 cgataaggtg atatcaccga tatctttaat tttatcgatt acatgtttgg ataaataggt
     5641 aattatgccg gttggggcgt agatagtgac ccgaccacgt ttctcatgct tataatctgc
     5701 aataaaatta ttaagttgct cgtttctatc cagcatgtcg ttgatgtacg gtaacagcgc
     5761 ggtgccaaaa ggtgtgagca tcagctgccg ggtggttcgg tcaaagactt ttaaacccac
     5821 ttttgattca aaatcagcaa gatatttgct gacgttggcc tgtgcgatgc caagtacagt
     5881 cgcggcatgg ctgatatttt cactggcagc gattaccgag ataattttta actcccggta
     5941 cttaagttgt aattttgtca tcgcaacacc ccaatt
//
LOCUS       DAZGAN020000196         5969 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGAN020000196 DAZGAN020000000
VERSION     DAZGAN020000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.196"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..153)
                     /locus_tag="ACHKYO_RS24075"
                     /old_locus_tag="pgaptmp_004815"
     CDS             complement(<1..153)
                     /locus_tag="ACHKYO_RS24075"
                     /old_locus_tag="pgaptmp_004815"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HGU4431502.1"
                     /translation="MSKSVDIFIGFLVTVLWGANFVVIDVGLRDLDPFALTFLRFLFC
                     AIPLCFF"
     gene            293..973
                     /locus_tag="ACHKYO_RS24080"
                     /old_locus_tag="pgaptmp_004816"
     CDS             293..973
                     /locus_tag="ACHKYO_RS24080"
                     /old_locus_tag="pgaptmp_004816"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431503.1"
                     /translation="MFDVQQHRYNYFYNMATVVENLIPEDICDTLVSRINELISEGAV
                     DHVKHSGLGNNAVSDLGGDYNHHIFKGPDIRKHLPELNAIYHAILPLISIITCTDTIT
                     SPYELSDVNIKAYPPNGGTLGLHYDTNGITVLLSLMDNKEAPLRIQIPRSHPSKKESW
                     IEHKEIYAKKGGLLIMQGRKVLHDSAPTQTEQKISVIYNYYERHDTYRHKDFDNFVYF
                     GIKPDDLE"
     gene            complement(1031..3250)
                     /gene="mobQ"
                     /locus_tag="ACHKYO_RS24085"
                     /old_locus_tag="pgaptmp_004817"
     CDS             complement(1031..3250)
                     /gene="mobQ"
                     /locus_tag="ACHKYO_RS24085"
                     /old_locus_tag="pgaptmp_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HGU4431504.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDERTGLTHDYTR
                     KRGVDDAVILTPENAPSWCADRTVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDRSNPHAHIMLTTRAVGETGFAGKVRDWNDRTL
                     AETWRASWADHANRALANAGYLEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLIDQLNQEIQLIRTKLALSQVQERMRKRELTDTARRAAEALNLTIPGDDASA
                     DTLKKFISTLPQECGKAWEMTPEFVAMNGKVNDTERESHALKKEQNILGKEMSGLRKA
                     RPVASILSEFPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSARETERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLNNRSGKFTVWGDELAHYPQGEGDPVNVYLSPGGA
                     VMVSDIRNGISESHETLARPERVRMYSGTTVRHLLEQMRHKWPSYGFPALPHHWPDNF
                     YFSDDRRPVTSPLPSADRVDVTAYSAPEQLMPVVFSVARNNHMLHLLLCKGPEEMFVG
                     FVRLEDGLRPVLALPSPDYSHLMVSTITENGIHLEGYGEAINRDADTPYPPKPHLMQF
                     RLKGCQDRLFAAINKPEEMPDYLFRQLGFNQTWHEWKQDEQHRQQQRRPGHYRGMSM"
     gene            3429..3704
                     /locus_tag="ACHKYO_RS24090"
                     /old_locus_tag="pgaptmp_004818"
     CDS             3429..3704
                     /locus_tag="ACHKYO_RS24090"
                     /old_locus_tag="pgaptmp_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HGU4431505.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADMNRLQPLINELEKLPKPPAKPQNH"
     gene            complement(3911..4846)
                     /locus_tag="ACHKYO_RS24095"
                     /old_locus_tag="pgaptmp_004819"
     CDS             complement(3911..4846)
                     /locus_tag="ACHKYO_RS24095"
                     /old_locus_tag="pgaptmp_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HGU4431506.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASARRSIDKHYGMGRNCYLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGKSIAKYTHRNFTPETFAQYVATTHTPEIQAARGRKGGIAKGAGYEDK
                     RSTALEMLVNGCTQKAIAEMLGVSDRTIRNWKSGK"
     gene            complement(5241..>5969)
                     /locus_tag="ACHKYO_RS24100"
                     /old_locus_tag="pgaptmp_004820"
     CDS             complement(5241..>5969)
                     /locus_tag="ACHKYO_RS24100"
                     /old_locus_tag="pgaptmp_004820"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HGU4431507.1"
                     /translation="IKRPREMSLVYVSIYGVIFGVGLWWLVNYAMYNGLAAGVSSVFL
                     QFSAFFTIILSAFIFGEKINKVHAVGMIMSFLGLVMMITSSDDSSTIYGIFLVILAAL
                     AWALCNMIVKMKKPSDMVGFIVWSSIFSAPSVLILTVIAKGWGPIISIKDNISFASAF
                     SVLFQAYITTVIGYMIWNNLMKKYPATEIAPLSLFVPVSGLTASFFLLDEKLSVFQLI
                     AVVVVCVGIFIFLNASRIKKWINL"
BASE COUNT         1643 a         1556 c         1337 g         1433 t
ORIGIN      
        1 aaaaaaacac aatggtattg cgcaaaataa aaatcgtaaa aaagttaaag caaaggggtc
       61 taaatccctt aatccaacat caatgacaac aaaattagct ccccacaaaa ccgtgaccaa
      121 aaaaccaata aagatatcaa cgctcttgct cataaatcac ctaaaaaaca ataagtaatc
      181 aacaatcaag gattacctac cacaaacaaa aaacaaacac aacaaaaaca ttgacacaac
      241 acaccatcac gattatgtta caaaaaatca acaaccataa aaggaaaaga atatgtttga
      301 tgtgcaacaa cacagatata actactttta taacatggca acagttgttg aaaacctgat
      361 acccgaagat atttgtgata ctctagtatc aagaataaac gaactaatca gtgaaggagc
      421 tgtagatcac gttaagcact caggattagg taacaatgca gtttcagatc ttggaggtga
      481 ctacaatcat catatattca aggggccaga tataaggaag catcttcctg agttaaacgc
      541 catatatcat gcaatcttac ctttaataag cattataaca tgcacagata ccatcacttc
      601 accttatgaa ctgtcagatg tcaacataaa agcataccct ccaaatggtg gaacattagg
      661 gttgcactac gacacaaatg gtataactgt tttattatct ctaatggata acaaagaagc
      721 tccattaaga atacaaattc ctagaagcca tccaagcaaa aaggaatcat ggatagaaca
      781 taaagaaata tatgcaaaaa aaggaggcct tcttataatg caaggaagaa aggtattaca
      841 tgatagcgcc ccgacccaaa cggagcaaaa aatatccgtt atttacaatt actatgaaag
      901 gcacgacact tatagacata aagattttga taattttgtc tattttggta taaaaccaga
      961 tgatttagaa tagtatgcat tatctggctg ttttaacaaa aatagccaga taaaatagtc
     1021 ttatttactt ttacatactc atgccccgat agtgtcccgg gcgtcgttgt tgttgcctgt
     1081 gctgttcgtc ctgcttccac tcatgccagg tctgattaaa cccgagttga cggaagagat
     1141 aatccggcat ctcttccggt ttgttgatgg ctgcaaaaag cctgtcctga cagcccttaa
     1201 gccggaactg catcaggtgc ggtttcggtg ggtacggagt atcagcatca cggtttatgg
     1261 cctcaccgta gccttccagg tgtatcccgt tctccgtgat ggtgctgacc atcagatgac
     1321 tgtaatccgg cgagggtaac gcaagaacag gacgcagccc gtcctccagg cgcacaaacc
     1381 cgacaaacat ttcctccggc cctttgcaca acaacagatg cagcatatgg ttgtttcgcg
     1441 ccaccgaaaa tacaacgggc atgagttgct ccggtgcgga ataagcggtg acgtccaccc
     1501 ggtccgctga cggcagcgga gaggtgacag gcctgcggtc gtcgctgaaa taaaaattat
     1561 ccggccagtg atgcggcagt gccggaaaac cgtaagaggg ccacttatgg cgcatctgtt
     1621 ccagcagatg acggaccgtc gtaccggaat acattctcac acgctcaggc cgggccagcg
     1681 tctcatgaga ttctgatatt ccattacgta tatccgagac catcacggcc ccgcctggcg
     1741 acaggtaaac attcacgggg tcaccttctc cctgcgggta atgtgccagc tcatcacccc
     1801 ataccgtgaa ttttcccgag cggttattca gcatcatgta acgggttatc cggcccgttc
     1861 ccggctctct ggcacgcccc agctccgtaa taaccccgac taatgccgtt tcacggctca
     1921 gacggtggtt atcatgacgt ttcagccgcg cctcatgctc ctgttccttc cggcgcgctt
     1981 ccgcttcggc ttcctccctg cgccgctccg tttcccgtgc tgacagtttt tctttcagct
     2041 ctgccattcc cggcgcaatc cacgtattcc actgcgtttc aaaggcctga cgacgggcgg
     2101 gaatatcccg ttctttcaca gccctgtagg tgcggcgaag agattcaatc tgtttcccta
     2161 gtttccagaa acggagttgt cttttgcgat attccggttc agcccatgtc atcaggggaa
     2221 actctgacag aatggacgct actggacggg cttttctgag tccgctcatc tcttttccga
     2281 gaatattttg ctctttcttc agcgcatgac tctcacgttc ggtgtcgttc accttcccgt
     2341 tcatcgccac gaactccggg gtcatctccc atgccttccc gcattcctgc ggcagtgtgg
     2401 aaatgaattt tttcagggta tccgctgagg cgtcatcgcc gggaatggtc aggttaaggg
     2461 cttcagctgc acgacgtgcg gtatcggtga gttcccgctt acgcattctc tcctgtacct
     2521 ggctgagcgc cagctttgtg cggataagct gtatttcctg gttaagctgg tcaatcagac
     2581 ggttctgttc accgcgttct gtttccattc cccgtttttc catcgcacag gcggccttcc
     2641 cgaggtgaag gccgggcgct ttctccagtc cctgacgctc gtatgaacga tggtctatct
     2701 cttccaggta gccggcgttc gcaagtgccc tgttcgcatg gtcagcccat gatgcacgcc
     2761 atgtttcggc gagtgtccgg tcgttccagt ccctgacctt tcctgcgaat cccgtttctc
     2821 cgacagctct cgtggtcagc atgatgtgcg catgggggtt actccggtcc atgtgatgga
     2881 acgccacatc tgcaatcatg ccctgactga caaagttttc ccggacaaag gcgagaacgg
     2941 tttcccgtgc ggcctcgcgg gaaatctcac ggggaatggc aagttcaatc tcccttgcca
     3001 gctgggagtt acggcgctgt tcagctttct cgaccgcatt ccagagaacg gtacggtcag
     3061 cacaccagga cggtgcattc tcaggggtga gaatgaccgc atcatcaacg ccccgtttac
     3121 gggtgtagtc atgggttaat ccggtgcgct catcgggaat gacggagcct gaacgatagg
     3181 cagcagaagc aacggcactg tagccgtttt ttcgcgaaat gattttcatg ctgagatgat
     3241 aaatcgccat ttctgccgta atgccttttt gccgttttct ttcagccccg cagggcgcac
     3301 acaatcgaaa cggagtggag attgtataag tgcgcacttc gtgatgttcc gtgcattatg
     3361 ccagttcagt ttttaaacta ccagtgacgc gataaaatgc gatttttcac cgcgcacgga
     3421 gtcacgaaat gagcagaaca ctcgaacaga agattgccga cgctgaggcc cgattacagc
     3481 gcctgaaggc gaagagccgg agcctggaca cagcgcaaaa agtcattgtg ggggcagcca
     3541 tgctggcaag ggtacggagg ccggaagagg cgcaactgcg agcgttcctg ctccagttcc
     3601 tgcgaaagga agtcacaaga caggccgata tgaaccggct acaaccgctg attaacgagc
     3661 tggagaagct acccaaacca cctgcgaaac cgcagaacca ctaagccccg gcgcgtaagt
     3721 gccacatgca ccaccggacg cctttgcgct tcctcgctca ttcgctcgct acgctcggtc
     3781 atgagactgc ggcgagcgct gccaccgcaa tcacgaggaa accagcgcca ttgctggacg
     3841 gaaccggaaa aaccacagga acaccaaaag ctcaaaaaag cgccgaaaag gcgctgttat
     3901 ctgatatagg ctattttccg cttttccagt ttcgaatagt gcgatcggaa acacctaaca
     3961 tttcagctat agccttttga gtacacccat tcaccaacat ttccagcgcg gtgctgcgct
     4021 tatcctcata gccagcccct ttggctatac cacctttacg gcctcgggca gcttgaattt
     4081 ccggcgtgtg cgttgtcgcc acatactgtg cgaaagtttc cggcgtaaag ttcctgtgcg
     4141 tgtatttcgc tatgctcttg ccaatagccc gacattcagc cggagaaagc ggaacgggaa
     4201 gtgatgcgtt gtacatttcg acacgctgga tcacggcatc aagccattgt gagaatacag
     4261 gccagccctg acgaatcgcc ctgtaagccc atttgcgcgt catttcgaac aggtagcagt
     4321 ttcgccccat cccgtaatgt ttatcgatgc tacggcgcgc tgaggcgctc aaatcgagat
     4381 aatcagccag ttcatcgagg gtataggctt cctcgcgcca ctccatcacc agccattcca
     4441 ggtggaacgg atttttgcaa atcagtccgc tgtagttcac atctgcacca agtttctcac
     4501 acagcgcacg ctctatcgcg gcagcgtact tcagtgcctt aaccgacgca tcaggcgcgg
     4561 ttctcacggc gatgttgagt gcatagagca ggtgagcatg accgttcacg ggatttttta
     4621 cggtgatgtt cggcgcgggg gcgttccggt cgctccagtc aatcgcagcc ccctcgcggt
     4681 caacgtcaaa aaccagccag aactgcgcat gaggctggtt ctgctggatg taccgcgcaa
     4741 gaagcgcacg ccctttgccg gagatgcgaa gaccaaaagc gagatcatcc gtgtgatagg
     4801 gacggtgggg taaattttct tcgaagtatt gaagcgcggc gctcatgtgt tacgattcct
     4861 tccaggttat cgaccgacct cgcccctgta ttctcgtgac aatcttggcg gagagacacg
     4921 aatttcaggg gcgtttgttt ttcagtcgct tacactacac ccaacggcaa atcctgtcga
     4981 accttcccga aaacccgatc catttaccat aactcccgtt gtacgtaccc cttaaaacgc
     5041 cctcagacgc tttctgacgc aaaaaactca aaaagacaag gaaacaccag gcaaagccag
     5101 aaaaacgcgt cagaattgtt ttcatgagcc tgcataacta ttcacgaaag cgatgcgcga
     5161 tcgatttcga ccgttccgaa gggcgaacgg gtcaaaatga caagtttctc tctccataga
     5221 aggttccgca cggggtttag ttataaattt atccattttt ttattctgga tgcgtttaaa
     5281 aagataaata taccgacaca gacaacaacc acagcgatta attgaaatac ggatagtttt
     5341 tcatctagca aaaagaatga tgctgtcaaa ccagatactg gaacgaacaa agacagaggg
     5401 gcaatttctg ttgctggata cttcttcatt aagttgttcc agatcatata ccctattact
     5461 gttgtaatat atgcctgaaa aagaacagaa aaagcagaag caaatgatat attatctttt
     5521 atagatataa tcggccccca gcctttagca ataacagtta aaatcaaaac agaaggagca
     5581 gaaaaaatac ttgaccaaac tatgaagcca accatatcag atggtttttt catcttaacg
     5641 atcatattgc acaacgccca tgccaacgca gccaatataa ctagaaaaat accataaata
     5701 gtagaactat catctgatga ggtgatcatc ataaccaacc ctaaaaaaga cataatcata
     5761 ccaaccgcat gaaccttatt tattttctcg ccgaatataa aagcacttaa aataattgta
     5821 aaaaatgcac taaattgtaa aaacacagaa gaaaccccag cagctaaccc attgtacatg
     5881 gcataattta caagccacca caacccgact ccaaaaatca caccataaat agacacatat
     5941 actaaagaca tttctctggg gcgtttaat
//
LOCUS       DAZGAN020000197         5964 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGAN020000197 DAZGAN020000000
VERSION     DAZGAN020000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.197"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(300..515)
                     /gene="cspB"
                     /locus_tag="ACHKYO_RS24105"
                     /old_locus_tag="pgaptmp_004821"
     CDS             complement(300..515)
                     /gene="cspB"
                     /locus_tag="ACHKYO_RS24105"
                     /old_locus_tag="pgaptmp_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HGU4431508.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
     gene            814..1016
                     /gene="cspF"
                     /locus_tag="ACHKYO_RS24110"
                     /old_locus_tag="pgaptmp_004822"
                     /pseudo
     CDS             814..1016
                     /gene="cspF"
                     /locus_tag="ACHKYO_RS24110"
                     /old_locus_tag="pgaptmp_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
     gene            complement(1191..1943)
                     /gene="ydfT"
                     /locus_tag="ACHKYO_RS24115"
                     /old_locus_tag="pgaptmp_004823"
     CDS             complement(1191..1943)
                     /gene="ydfT"
                     /locus_tag="ACHKYO_RS24115"
                     /old_locus_tag="pgaptmp_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4431509.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDAQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGDADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(1957..3006)
                     /gene="ydfU"
                     /locus_tag="ACHKYO_RS24120"
                     /old_locus_tag="pgaptmp_004824"
     CDS             complement(1957..3006)
                     /gene="ydfU"
                     /locus_tag="ACHKYO_RS24120"
                     /old_locus_tag="pgaptmp_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4431510.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKTPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEEKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            complement(3008..3286)
                     /locus_tag="ACHKYO_RS24125"
                     /old_locus_tag="pgaptmp_004825"
     CDS             complement(3008..3286)
                     /locus_tag="ACHKYO_RS24125"
                     /old_locus_tag="pgaptmp_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431511.1"
                     /translation="MARNVKYYNSDNSPVLVCTHERYSHAFKSEWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            complement(3353..3604)
                     /gene="rem"
                     /locus_tag="ACHKYO_RS24130"
                     /old_locus_tag="pgaptmp_004826"
     CDS             complement(3353..3604)
                     /gene="rem"
                     /locus_tag="ACHKYO_RS24130"
                     /old_locus_tag="pgaptmp_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HGU4431512.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDGRVTDGFILREGEFVTSLQALKEIGIKAGFSAFAEE"
     gene            complement(3821..3976)
                     /gene="hokD"
                     /locus_tag="ACHKYO_RS24135"
                     /old_locus_tag="pgaptmp_004827"
     CDS             complement(3821..3976)
                     /gene="hokD"
                     /locus_tag="ACHKYO_RS24135"
                     /old_locus_tag="pgaptmp_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU4431513.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(4595..5848)
                     /locus_tag="ACHKYO_RS24140"
                     /old_locus_tag="pgaptmp_004828"
     CDS             complement(4595..5848)
                     /locus_tag="ACHKYO_RS24140"
                     /old_locus_tag="pgaptmp_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431514.1"
                     /translation="MSKNLPVLLSSPSTTNFPATVNTEIEFLSQARKLLDSGFPDHAL
                     LDIWNASIHNLRRRIEAYGLDLFLSAIKDDSGRKKYDKDGETINERWSGVDDLVLISG
                     ATKLGVLHKKAGKSLEMINWMRNHASPAHASDSKVESEDVFALALMLQKNLFESDMPD
                     PGHSPSGLFEPIKKSELSIESIDLLKDQIRAFKQADIRITFGFLLDLITKGENPAYVN
                     ASKLFEQAWERSNDDLKKTAGERYHSFMLEPESDTSEDKGAKIRILETLVKVNGVKFI
                     PDAARASLYRHAVKQLAKAKDTSYGWNDEERAAKALIQFGPHVPSIAFEDVYQEILTV
                     WVGNYWGRSDAHLVLQPFLDVLNSNQLMALSRLFVENERANSELVSSRPKRRAIELLE
                     IIKNNLTIQASIDEIDRIISEIDKM"
BASE COUNT         1616 a         1441 c         1165 g         1742 t
ORIGIN      
        1 acccgcctga gcgggtttga gattgtggtg ctttttgtgg gagtcatcca cttacgcact
       61 ttgttttgcg atgccagcag ttagcttctg ctgtgaaact attcatgcag caaacctgca
      121 cttcaccaca atggttagca tacttttcct gattaagatt ttgccaaata tgctggccat
      181 tgtttcatgt attggacctc cttacttttt attaaagaga tccaatattc actactctgt
      241 ccgtatctct actcaggcat cagccttctt cgttatcgta tacagacgag cgatgaattt
      301 taatcagtaa tgatgacatt tgctgctgca ggacctttag caccactctc tatagagaag
      361 gtaacctttt gaccttcaaa taaggttcga taattatcat tctgaatcgc agaaaaatgc
      421 acaaacacat ctttactacc atcaacagga gaaataaagc cgaaaccttt atcagcgtta
      481 aaccatttta ctaaaccagt cattttattt gacattctac attccttaac ttgagccttt
      541 cggcataaat ggtttgcata acagaaacga cttcgtactt aattggagag actcaaagaa
      601 ggaataagtg aataacacct gaaatgagaa ctgctttagt aaactacttc gtatatcgtc
      661 tgttcttcaa accgacacag tcattaactc atagttgaac atatgaagca atgtttattt
      721 tagacatcca gccaccttca accctatcaa aaaagtagtt ttctccagga acgtgtgtat
      781 ggtgcaccag gttatcagta ttaaggagtt tttctgtccc ctaaaatgac aggaattgtc
      841 aaaaactttg actacaaaag cagcaaaggt cttatattcc catccgatgg cgtatcgatg
      901 cccagcttca cgtttcagct ctcaatcttc gagatgcaga agaaattacc acaggattac
      961 gcgtggaatt ttgctggata aatggttcgc gtggaccttc aactgccaat gtttatcccg
     1021 ggatgagatt caatatctct attgccccat ttaaagcaca aaaacccgct tataagcggg
     1081 ttttctactt ttttctaaac gtcggataca caaagcccat cgttgagaaa atcttatcca
     1141 tgttttttga aaaatgcaaa catcatgtcg ccatcttcag caaaaatcat ttatctcgtc
     1201 accttcctca attgcgcttc cgcgtatgct tcttcctgcc agcactttgt taccagttta
     1261 ccaatgacgt ccgcatcccc cttataccac tgataatcgg tcaggtctgg taccagcttc
     1321 tggacatgac gtcgtgccag cgtggtcggt aaacgactaa accggtttcc attacaacgc
     1381 ccacaaatct tatataccgg tacgccatga aaccgggttc ttttttcatc cagaacaatc
     1441 cctttaccct tacaccctct gcacgctgtg ctgacttcgc ccttaccatg gcaatgctga
     1501 catagttcct tcacccattc ttccttgatt acagattccc cgcgtctgta gtgtttcacc
     1561 acttcgcgca atacattata aaatcccgta cctgaacaat gctcacagcg agccttactt
     1621 gccgcagacc tggagtaatc agcaaaggca aaattcacga ggtaaggaat aatctgtaac
     1681 cggatttctt cactcaattt gttcaatgtc gggttatcca gtgccatcgc gtaatttagc
     1741 aggccttcaa tcgcaaactg agcgtcctga acaccaactt ttgccagaaa taaggccaac
     1801 ccaagtggtg ctttcgactg caccatcccc tgcgctgcca ttacatccgt aattgttaaa
     1861 caaccggtgc ctgtcgctgg agcgtcatcg ctcaattttg gagattttgg ggagtaatat
     1921 tttggtaagg cttcaaggtt catgctcgtt ctccacttac gccagtacgc caattgccag
     1981 cgcgcgatcg ataaaacgaa atatcagctc cagttgggag ccatacttct cttcaaatgc
     2041 cactgtatcc gtatgcagct cgttgtgatg ctttctgcac aaaggcaaca caaagagatc
     2101 atgtgctttt gttcccattc cgccctgccc gtgaccaatc agatgatgcg gatcgtcggc
     2161 tggcataccg cagcaagcac acggctgtgt cttaacccaa cgtgtgtatt tctccttaac
     2221 ccagcggcga cgtttaggca gcttcatgaa agattccgga gactctggat caacggtgat
     2281 gcttaccacc gtcttttcct gtggtgtttt ttgttgctgg tgggcgtaag gcaacggtgc
     2341 aagatttttt gtgcgttgtt tcaatatgct ggtggcggtc tgctctcccg gtacgatgtc
     2401 gctttcgcgg tacaccgagc agattttttc cgctggtaat cccagcgaac gacgcgatac
     2461 agcctcaggt agtgcatcca ccacctgatt gcagaccgcc caccaggata attcagccaa
     2521 agataattcc cgctcctgcg taccgcttat tgcgtgacgg atgacgtcaa tcacccatgc
     2581 tgtcagattt tgttgagcaa gcagctccag tgattccgat gtctggtcac gcagttggtt
     2641 gtcgcagtgc caacacaaca ccattgcgcc ggtaccataa cggtgaatga ctgtttcaga
     2701 gtgatggtaa tcgccattag gccactggca ggatgtaaca tgacgtaata gccagtcaga
     2761 caatgcgcca acaccgccag cagcacgaat cacccgttcg ttactaaaaa acggcagcaa
     2821 tgttttgtct tccgccagcg gctggcgaac ggcaggaacg actccggatg gcagattacg
     2881 catgcttttt ggttccggtt ccaccagcac tcgaggatta tgaaatatct gtatggattc
     2941 acggcccggc ttaaggacca ccagcccaag ctcaggcacc agaacaggtc taagtaatac
     3001 ccgcacgtta cctccagatc cgttgctgga aagtgcggga cgcacgtggt gggcgttcgg
     3061 agtaaggcaa tctgactgag attatccagt gacggtagtc gaggctaagg gctttcttaa
     3121 cctcgtatcc gcgcctgcgg taacactgaa ttatccattc agcctgctct tcagtgcatg
     3181 gagggtgttg gaaccattca gacttgaatg cgtgagaata ccgctcgtgc gtgcagacaa
     3241 gaacgggcga attatcagaa ttgtaatatt ttacgttgcg tgccatcggt tttctccggt
     3301 ggcacggtgt tactcagcgg gagttcagcc ccgcgcaaga ttgtagatga gtttattctt
     3361 ctgcaaaagc tgaaaagcct gcttttattc cgatctcttt cagtgcctgt aatgaagtga
     3421 caaactcacc ttcgcgcaag ataaatccgt ctgtcactcg accatccaca aaattaatta
     3481 acgcagcccc attctttcgc aaacacataa tgcggtaatg actaacaaga tttccatttt
     3541 caacgcacac agcatagagg ccatcttcac aaaaaatttt acgcagttct tcgatgttca
     3601 tcatcagaat ccttccggat aattagctct cccctttaag ggaccatccc tcttatccct
     3661 gcgcgctact taagtatttt tgattctatt ccggcaccgt ccagaacttc aaacgcgttg
     3721 aaaataaaaa caaaaacccg ccgaagcggg ttaagtgcgg gtgcgttgag gatgcctgcc
     3781 acatcagagg tggcgaggga tttctccctc gccgggtctc ttactcctca ggttcgtaag
     3841 ctgtgaagac agcgacctcc gtctggccgg ttcggattcg tacctcgcag aggtctttcc
     3901 tcgttaccag tgccgtcact atgacggtta aacagatgac gatcagggcg attaacatcg
     3961 ccttttgctg cttcatagcc tgcttctcct tgcctttcgg cacgtaagag gctaacctac
     4021 atatgtctag catgaaattg gcctcagatt aatgttaagc gtcttgcagg acgcgtaatg
     4081 ttaactgggg cttttctcta tctgccgttg gtgttcatgc ccgaggcaga tagcctcaag
     4141 cacccgcagc cattctactt aactaccgtt acctcgccaa tgtgaaatca gtcagaaagg
     4201 cgatccataa gaacaatagc aagacaataa atcgccatta cagccgtaat agccagcgca
     4261 catttgagaa ccagcaccac aacctcctgt attggacgta caccagtcct gatgaatatg
     4321 aggctgtctc gtcagtgatt caatacaact attgggtata gtttctctga ttttttctgt
     4381 ggaaatgggg ctcaaccact agtcatttaa tacgcaaagt ccgacacaag ctaaccttct
     4441 agtccgcttt gagcgagaag cgggcctttc gacaaccata tttagatgaa tagtgggcta
     4501 ggaatacttt agcaattaca ttgctaatat caatataagt agtagattca aaaaaaataa
     4561 gccacctaaa aagcatggct taattgcaaa taaattacat tttatcaatt tcagaaataa
     4621 tcctatcaat ttcatcaatg cttgcttgaa ttgttagatt attttttatt atctcaagta
     4681 attctattgc tcttcttttg ggccttgatg acaccagttc tgaatttgct ctttcattct
     4741 ccacgaacaa ccgtgagaga gccatcaatt gattactatt taacacatct aaaaatggtt
     4801 gaagtactaa atgtgcatca gatcttcccc agtaattccc cacccaaaca gtaagtattt
     4861 cttgatatac atcttcaaaa gcaatagatg gaacatgagg tccaaattgt ataagtgcct
     4921 tggctgctct ttcttcatca ttccagccgt aagatgtatc cttagcttta gctagctgtt
     4981 taaccgcatg tctgtataaa cttgccctag cagcatcagg tataaatttc acaccattaa
     5041 cttttactaa agtctctaaa attcttattt ttgctccttt atcctcactt gtatccgatt
     5101 cgggctccag cataaatgag tgatatcttt cccccgctgt ttttttaagg tcatcattag
     5161 atctttccca tgcttgttca aacaacttgc tagcattaac ataagcagga ttttccccct
     5221 tagttattaa atccaataaa aatccaaatg ttattcttat atcagcttgc ttaaatgctc
     5281 ttatttgatc tttaagtaag tctatgctct caattgatag ttcagatttt tttatgggtt
     5341 caaataatcc agatggcgaa tgtccagggt ctggcatatc agattcgaat aaattcttct
     5401 ggagcataag agccaaagca aacacatcct cagactctac tttactatca cttgcatggg
     5461 ctggagaggc atggtttctc atccaattga tcatttctaa tgattttcca gctttcttat
     5521 gcagaacccc taatttggtg gctccactta ttagcactaa atcatctaca cctgaccatc
     5581 tctcgtttat tgtttcacca tctttatcat attttttacg accactatca tcctttattg
     5641 cggaaaggaa taagtctaaa ccataagcct caattcgccg cctaagatta tgaatggatg
     5701 cattccatat atctaaaaga gcatgatcag ggaagccaga atctaacaac tttctagctt
     5761 gcgaaaggaa ttctatttca gtattaactg ttgcaggaaa attagttgtt gatggcgagc
     5821 ttaataaaac cgggagattt tttgacattg aatttccttt aagtaaaaat tataatctat
     5881 gtctttgtga acaaatttac acgatattgt aaccttgcag gtgcaagaag tagtgttcaa
     5941 ataaatactt gaagagaaaa aatc
//
LOCUS       DAZGAN020000198         5787 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGAN020000198 DAZGAN020000000
VERSION     DAZGAN020000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.198"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..147
                     /locus_tag="ACHKYO_RS24145"
                     /old_locus_tag="pgaptmp_004829"
     CDS             <1..147
                     /locus_tag="ACHKYO_RS24145"
                     /old_locus_tag="pgaptmp_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HGU4431515.1"
                     /translation="VLQSLMGHKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSML
                     KRIP"
     gene            327..971
                     /locus_tag="ACHKYO_RS24150"
                     /old_locus_tag="pgaptmp_004830"
     CDS             327..971
                     /locus_tag="ACHKYO_RS24150"
                     /old_locus_tag="pgaptmp_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HGU4431516.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            1058..1366
                     /locus_tag="ACHKYO_RS24155"
                     /old_locus_tag="pgaptmp_004831"
     CDS             1058..1366
                     /locus_tag="ACHKYO_RS24155"
                     /old_locus_tag="pgaptmp_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HGU4431517.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATVPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            1778..2758
                     /locus_tag="ACHKYO_RS24160"
                     /old_locus_tag="pgaptmp_004832"
     CDS             1778..2758
                     /locus_tag="ACHKYO_RS24160"
                     /old_locus_tag="pgaptmp_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HGU4431518.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGGDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            2751..3167
                     /locus_tag="ACHKYO_RS24165"
                     /old_locus_tag="pgaptmp_004833"
     CDS             2751..3167
                     /locus_tag="ACHKYO_RS24165"
                     /old_locus_tag="pgaptmp_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGU4431519.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(3169..4443)
                     /gene="umuC"
                     /locus_tag="ACHKYO_RS24170"
                     /old_locus_tag="pgaptmp_004834"
     CDS             complement(3169..4443)
                     /gene="umuC"
                     /locus_tag="ACHKYO_RS24170"
                     /old_locus_tag="pgaptmp_004834"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HGU4431520.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMHLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVESYSIDE
                     MFIDLREVNRCISPEVFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLTQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPHKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(4443..4880)
                     /gene="umuD"
                     /locus_tag="ACHKYO_RS24175"
                     /old_locus_tag="pgaptmp_004835"
     CDS             complement(4443..4880)
                     /gene="umuD"
                     /locus_tag="ACHKYO_RS24175"
                     /old_locus_tag="pgaptmp_004835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HGU4431521.1"
                     /translation="MSTVYHRPAAPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(4877..5125)
                     /locus_tag="ACHKYO_RS24180"
                     /old_locus_tag="pgaptmp_004836"
     CDS             complement(4877..5125)
                     /locus_tag="ACHKYO_RS24180"
                     /old_locus_tag="pgaptmp_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4431522.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            5409..5720
                     /locus_tag="ACHKYO_RS24185"
                     /old_locus_tag="pgaptmp_004837"
     CDS             5409..5720
                     /locus_tag="ACHKYO_RS24185"
                     /old_locus_tag="pgaptmp_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431523.1"
                     /translation="MNYDGDGRATARFFSGKGCRRAPLFTAPADVARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
BASE COUNT         1422 a         1334 c         1413 g         1618 t
ORIGIN      
        1 gtcctgcaga gcctgatggg gcataagtcc atcagctcaa cggaggtcta cacgaaggtg
       61 tttgcgctgg atgtggctgc acggcaccgg gtgcagtttt cgatgcctga gtccgatgcg
      121 gtgtctatgc tcaaaagaat tccttaagct ctgacttttt taaatcccct taggatatct
      181 catggatata ttatcggtat ttcatattct gttgacatgc aggaccggta tagagtaaaa
      241 aaaactctat tgagtacctc ttggatatcc gaaaggtgtc ttttgggttc tttatgggta
      301 ttacatgtgg attctggaga tacactatgc ctgttattgc taatacacat cccaaaggtg
      361 gtgtgggtaa gacaacttca tccgtaaaca ttgttggtga aatgaagtct gatacagttg
      421 atctggatac tcataccggg ctttctatca ttctggggct gagacctgaa ggaaaagaaa
      481 tttccgtgaa agtacccaaa acagtggatg agttaatcga aattatgact ccctacaaga
      541 acagcgataa gacgttactt attgactgtg ggggatttga ctcagatctt acccgtactg
      601 cgattgcctt tgcagactgc gttattgtcc cttcaaaaga ctccctgact gagcgtattg
      661 gtttgatgca ttttgatgga gtactggacg aaatcagctc tatcatgggg accgatataa
      721 ctgctcacct gtatctctgt aaagttaatc ctaacaagaa gaaattccct aagcttgatg
      781 caatcctgcc atcgttcaaa catctgaagc tgatgaagag ccgcatttct gctcgtgcag
      841 aatttgagga tgtcattgag actgggatgg gaatcactga gtctgttcat gggcgctatt
      901 cggcaggagg taaggaagtt attgccctta tcgaagaaat caatcatctg attgaaaata
      961 acaaacagta aagtgtatct attgggtatc catatagtat cctttgggct tttaatggat
     1021 atcttttgaa tatctaacaa acatcctggg ttatctcatg agcaaagcga aatttagtgc
     1081 acaggctgaa aaactgaaaa acttggtgga ttctgccaga ttacaaactt cttcttctgc
     1141 cgtaacagca acagtcccgt ctcagccaaa tgaaggtgca gaggaagttt caggtcgtcc
     1201 cgtggccgcc aggacgaagg ctaagaatct taaggctata ccgctgtcgt attttgaagc
     1261 tcatgcggaa cttaaagcga ccagtaaaac atctctggat tttagctcat atattattga
     1321 ggctataaga gagaagcttg aacgtgatgg tgcaataggt caataaacta tcagatacct
     1381 taagggtatc tgatgtgtgt cttttaaatg cgttataggg attccttgtg tacctaaaga
     1441 gtccctatta gacatctttc tgtatatggt gacatgattc gtcactgctt tcctttcctc
     1501 tcagtttcac catttcattc ccttccctta acctgaacca tgatttcaaa gatgttttta
     1561 tattgcattt atgtgttgca tataacattc ataatgtttt tgtatctatg atttctctgg
     1621 tcttaaggtt acatgtgcaa tatacatgca atttattgtg tttttatgtt gctttcaccc
     1681 tttcagaaag accatctttc cattaaaatt gaatatacat tgcatgtatg aagtgtgttt
     1741 gttttttcta tttgtttatg agcaacggag actggttatg aaaatcttta ttgatgatgg
     1801 ttctaccaac attaaacttg cctggttgga ggatggtgac gtgaaaacgt taatcagccc
     1861 taacagcttt aaaccggaat ggtcttttag tttactggat gatgctgccc cggctaatta
     1921 cgagatcgac ggcgagaagt tttcatttga tccattaagt gctgatgctg tcgtaacgac
     1981 tgaaacacgc tatcagtaca gtgatgtgaa tgttgtggcc attcagcatg cgctacagca
     2041 gaccggcctg aaagcgcagc ctgttgatgt catcgtcaca ttgccgattt ctgaatacct
     2101 tgacgccaac aatcagaaaa ataagcaaaa cattgaaaga aagaaaaaaa atgtgatgcg
     2161 tgaagtacgg gtgcaggggg gggatgcatt tgttattcgc agtgtttccg ttcttcctga
     2221 aagtattccc gctggtttca gtgttcttgc ggggcttgag gatgatgaat cccttttgat
     2281 cgtagatctc ggtggtacaa ctcttgatgt gtcgcatgtt cgtagcaaaa tgacagggat
     2341 tacaaaaacc tggtgtgatc cgaatatcgg cgtgtccctg attacatccg gggttaagga
     2401 acagatggct gtacatgcta atacaagagt gagctcattc caggctgaca atatcattgt
     2461 gcaccgtaat gaacctgact acctttcccg tcgcatttat aatgcggaac agcgtgagtc
     2521 tatcattaat gttatcaatg aacggcagaa gttgcttatt aaacgagtta atgatgtcat
     2581 cagccgtttc accgactata cacatgtaat gtgtgttggt ggtggggctg aaattgtggc
     2641 cgaggctgtg aagaacctga ctaaggttcc tgatgagcgc ttttatctga gctcatcccc
     2701 gcagtttgat ttagtcatgg gaatgataaa aatgaaaggt ggtgtgacta atgagtgatg
     2761 aaaataaatc cagacgctgc tcatttgaac ttttcccgga tgaacggaca ggagataaga
     2821 tagcagatga gctcattgca aatgaaaaac tgaaggagcg gggacgtttc atgcgcgcga
     2881 tgttagttac tggtgcggcg tttgcagcta tagataaacg tcttcctttg ctgatatcag
     2941 agttactgac agaaaatacg actctggatg atattaataa ggtgatttcc agcgtaatac
     3001 caggtgcttt ttcggttgaa aagaaattgc ttgaattact ggaaaaacaa tctggtctcc
     3061 atacgtctgt ggattgcagt actcctctta cagagcagag tctgtcgaga aatgacggtg
     3121 aagaccagac cagaagaaac gctgaaaata tgttcggaga tgactgaact aacccagcct
     3181 ggccagggga atatctcgcc atttagttgt ataacactga ctcaacattt cccgtttcat
     3241 ttgccattca ggggcggttc ctcgccccgc aaaccacact ttcccttttc ccgactggtt
     3301 cagttcatcg agtgttttca ttaacttttc gctgttttta tggggctgga tctcatcaaa
     3361 taatcccggc tgcgctatac ccgatggtgt gaaatcagcc agcatgactc ctgccttcat
     3421 atagcggtac ccttcacgcc agacatggtt taaggctctg catgcggcgg caataatgtc
     3481 ccggctgtcc tgtgtgggca atggaagctt ttccacagcg gcattgctgt aacagggttc
     3541 ttttactgca aagggggatg tccgtacaaa tgtcgtcacc tgccggcaat actgacgctc
     3601 cccacgtagt ttctctgcag cccgctctgc atactgaaca acagcctggt gcatggcatc
     3661 tttgtctgtg attcgttcac caaaactgcg actacagaca atctgctgtt ttgccggtgg
     3721 tgcttcttcc agggatatgc aggactcgcc gttgagttcg cgtaccgtac gctcaagaat
     3781 gacgctgaag tttttccgga tgaatgccgt gttagcctgc gtcagctgca gtgctgtgtt
     3841 aatacccagc gcattcagct tttccgtcag tctgcgtcct actccccaga cttcaccaac
     3901 tggctgcagc cccagtagct tcaagatccg attacggttt tctgccgtca gcgcgaccac
     3961 accggaaaac tgtggccatt gctttgttgc ccactgtgca cttttagcca gcgtttttgt
     4021 aggtgcaatg cccaccccca tggtgagtcc tgtccagctc tttacctgtt ccctgagctg
     4081 atgaccaaaa acctccggag agatgcaacg gttcacctcc cgcaaatcaa tgaacatttc
     4141 atcaattgag taggactcaa ctgcgggaga aagcgactcc agaacagcca taacccgttg
     4201 gctcatgctg tggtacagcg cataattgct ggaaaataca tgtattttct tctccaggtg
     4261 catttgtctg acctgaaacc agggctgccc cattctgatg ccaagggctt ttgcctccgg
     4321 gctgcgcgcg atcacacagc catcgttatt gctgagtacg atgaccggtt cgttacgaag
     4381 gtccgggcgg aaaacttttt cacatgaggc gtagaaactg ttgatatcag ccagtgcaaa
     4441 catcagcgta actcccgcgt cctgtgtatc acgtgagtga caacaccaaa aatacagatg
     4501 ttttccggat acagtgtgcg gaactccggg ctgtctgaaa ccggctccag ggccgggcgt
     4561 gggcgcaaca gcagtcgttt gacggtgaac tcaccgtcga tctcagcgat aacgatgtcc
     4621 ccgtgttgtg gtttttcggc cctgtccact accagcagat caccattctg cacgccagcc
     4681 tggttcatcg attcaccgct ggcgcgcaga aagaaggtgg ctgcaggtct gctgatgcaa
     4741 tagctgttca gatccagttc ctgctcagca taatcagtgg caggagaagg aaaaccggcc
     4801 tggcaacgat cggcaaacaa cggtcgcacg taactgtcat cgcctgaagg ggcagccgga
     4861 cggtgataaa cggtactcat gctgcaggca gccagctgtc gtcctgccag atttcttcca
     4921 gcagagacag gatacgttct ttatccttgt cgttgcgggc tcctgtgaca gatacagacg
     4981 gggcgctgcc ctggtctata cgcagccaga tttccgggta gtgtggtttc agacgttgtt
     5041 cgatttcatt ctgtagcgcc tgaagtgtgc ctgatttgag gttttttgtg ctctggcggt
     5101 cgaaaagaat ttcaatgcgg atcatgttct catctccctt ttcttttgct gtatgtatat
     5161 acagcattct aataactgaa tccacacaca gtcaaatgtc attattgtga ctaaaaaacg
     5221 ctcaattctc tgagagtgtg gctatatgcc tgctgtgggc tgctgtgtcc agcttatcca
     5281 cagcattttg tgcacggttc tgtggacaaa atacctggtt acccaggccg tgccggcacg
     5341 ttaaccgggc tgcatccgat gcaagtgtgt cgctgtcgcc ggcctcctca cccggtcact
     5401 tacggtggat gaattatgac ggagacgggc gggcaaccgc ccgtttcttt tccggtaaag
     5461 gatgtcgccg tgctcctctt tttactgcgc cggctgacgt ggcgcggcat aaacgctgcc
     5521 tgtggtcagt gtcccgcgtc cggcgggcac gggacgggcg cttttaccgc tcccggctgg
     5581 cgtcggtgac agtgtacgcc agcccgtcgc ccttttctga tgaacgccct tcaagccgct
     5641 ttcgcggcat agccttgccg tcaaaaagac gacggctgcg gtattccacg gtcggcctga
     5701 cccgttaccg gacgcggtga acagcccaca ggcagcgggg aacgggcgct acaggggcgc
     5761 cctcccggtg ctctctgctg aagcaga
//
LOCUS       DAZGAN020000199         5757 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGAN020000199 DAZGAN020000000
VERSION     DAZGAN020000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.199"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1418)
                     /gene="ygcU"
                     /locus_tag="ACHKYO_RS24190"
                     /old_locus_tag="pgaptmp_004838"
     CDS             complement(<1..1418)
                     /gene="ygcU"
                     /locus_tag="ACHKYO_RS24190"
                     /old_locus_tag="pgaptmp_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HGU4431524.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNG"
     gene            complement(1488..2273)
                     /gene="ygcW"
                     /locus_tag="ACHKYO_RS24195"
                     /old_locus_tag="pgaptmp_004839"
     CDS             complement(1488..2273)
                     /gene="ygcW"
                     /locus_tag="ACHKYO_RS24195"
                     /old_locus_tag="pgaptmp_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4431525.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            2592..3869
                     /gene="yqcE"
                     /locus_tag="ACHKYO_RS24200"
                     /old_locus_tag="pgaptmp_004840"
     CDS             2592..3869
                     /gene="yqcE"
                     /locus_tag="ACHKYO_RS24200"
                     /old_locus_tag="pgaptmp_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HGU4431526.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSLVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            3896..5374
                     /gene="ygcE"
                     /locus_tag="ACHKYO_RS24205"
                     /old_locus_tag="pgaptmp_004841"
     CDS             3896..5374
                     /gene="ygcE"
                     /locus_tag="ACHKYO_RS24205"
                     /old_locus_tag="pgaptmp_004841"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HGU4431527.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPYAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGLITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLSESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         1482 a         1353 c         1381 g         1541 t
ORIGIN      
        1 atgccattag gatcgaactg ctttttcagc ccttccagca acgcccacgc gctgccgtgt
       61 tccagtttgc tccagtgaac gcgatgttta ccgataccgt ggtggtgcac catcgaaccg
      121 ccgaggcgaa tggtttcttc acagatgatc ttgttgagcg gattgtggta cttgtcgatt
      181 tcctcttccg gcttacagtc aacgacgttg taatcgtaga cgaagtacat gttggtgccg
      241 ttctgatagc tatgagagga atgaccgccc agcatggtga tgtcgtcggc gtgcgggaac
      301 tcagtacgaa tacggttaat aacgctttcg tagatttcgt ggatgcagct ccagcagccg
      361 gacacttcag tggtaaagcc catgttgccg gttttgagga tctgcacacg ttcggcagcc
      421 actttatccg gtccccagtt caggttgtta aaccaggttt cgatcagctt gctgtccacg
      481 cgctggcatt gcgggtagcg ggcaacgatt tccgcaatcc cttcgcccgt cgccttcgca
      541 atgcgagggt taccttcagc cataaagatc agcacgcatt ttccgtctgc aaaatgggtg
      601 aagtgttggg tgccatcttc agcgtcatac aaacgagcga tcgacggacg atacccttcc
      661 accatgactt cacgcaggat gttgaagccg gttttcatgt cttccaggat atagccgtag
      721 aagaggttgt tttccggggt gaatttaaag attttcactg ttacttcagt gatatagcaa
      781 agtgcacctt cgttaccgat gatgatgtgg cgaatgtccg ggccagccgc gcggcgtggc
      841 acgtttttaa tgcgtgtgac ggtgccatca gccaatactg cttccagacc aacgaccata
      901 tcttcgattg cgccgtagag tgtggagaac tgcccgatac tgcgggttgc taccaggccg
      961 cccatctgag ccagcggctt tgactgcgga gaatgccccg tggtgtaacc tttttcacgc
     1021 aacgcatttt ccagcacctc cagcggaacg ccacattgcg ccgtcgcctg catattctca
     1081 atatcaatat taatgatttg attcatggcg gagccgtcga gcaccaccga gttttccaca
     1141 acggtttcca gcccaccttc agtggcggaa gcgccggtac gcggcacacc gttaattttg
     1201 tgcgcattca taaaattcag cacacgggat acttgctctg tggaaccgag ttttacgacc
     1261 gctgccggaa tcggcaaagt ataaatgcca tgaatatccg gaaatttacg aaaacggtca
     1321 atactgttct tctttaatac tgtttcatcg gtaattacgc gatctgcacc aacaatttcc
     1381 tttagttggt cgacaatcgc tgcgcgagat aaagacatag taattccttc ctgataataa
     1441 aaatggaata atcattaata agagaaaaca gaaaccaccg ttatggatta gcgcactaaa
     1501 taaccaccat cgaccactaa taaatgccca ttgacataat ttgaagccgg acttgcgagg
     1561 aatacagctg cgcccattaa atcctgagta tcgccccaac ggtttgccgg aatatgatca
     1621 agaacgcgct gattggtttc tggattactg cgtgtcgcca gcgtaatatc tgttgcataa
     1681 tagccagggg caataccatt tacctgaata ttatattgac ctagttcatc acaataagct
     1741 ttggtgaatc cggcaagagc atgtttagtg gcagaatatg caggtgacca ttgtccacct
     1801 aagtaagaga acaatgaaca gatattaata attttgccgc ttttctgcgg gatcataatt
     1861 tttgcagctt catagcttaa ctcgaatgcg gcggtcaggt tcacatcaat catcggatcc
     1921 cagtcggcac gaccgaagtc cagcacctta ttcagcttac aaataccggc attgtttacc
     1981 agaatatcaa ctgtaccgaa acgctcacag caagcagcga taatcttctg cggcgcgcct
     2041 tctgcggtga tatccacctg catgaagtcc acctcaacac cctgtttttc aatcatttcc
     2101 tttgtttcgc cgttatcttt gacgaaacta ggaataaaga tatttgcgcc agctttggcc
     2161 aacgccatgg caaatgcctg gcctaaaccg ctattcccac cggtaacaat tgcggtttta
     2221 cctttcagtg agaaaaaatc cattgagaac gcattgagag attcgattga catagttagc
     2281 tccaatttcc gtaaggcaaa aaaaaagaaa ggtaacttcc cccgcgagag ggaagttacc
     2341 tttctcatta tcgctggtaa ctggagataa tttttagcat aacggcataa ctgctaatgt
     2401 gatcgtaatc acagtgtgat attcgtattg tcaaatagtt ttaaaaagca gaagcgtgat
     2461 tactctcaca caacacctct gctttccatg ttatttctta ttgcaattac tagagacctg
     2521 agaaaagtaa tccacctgaa agggcggctt acttttctct tttttttgtc tattacaaat
     2581 gggaatacga tatgcaacac aactcatatc gccgttggat aaccctcgcg ataattagtt
     2641 ttagcggcgg cgttagtttt gacctggctt atttacgtta tatttatcaa attcccatgg
     2701 cgaaatttat gggattcagc aataccgaga taggtttaat aatgagtacc tttggtattg
     2761 cggctattat tctttatgcc cccagcggcg ttattgccga taaattttca caccgcaaaa
     2821 tgattacttc cgcgatgatc attaccggat tactgggtct gttaatggca acgtatccac
     2881 cgctgtgggt aatgctctgt attcaggtcg cctttgcgat aacgacgatt ttaatgctgt
     2941 ggtcggtgtc gattaaagcc gcctcgttgc tgggcgatca tagtgagcaa gggaaaatta
     3001 tgggctggat ggaagggctg cgcggcgtcg gtgtaatgtc gctggcggtg tttaccatgt
     3061 gggtcttttc tcgctttgca ccggatgaca gcgccagcct gaaaacagtc attatcatct
     3121 acagtttggt ttacatcttg ttggggattc tgtgctggtt ttttgttagc gataacaaca
     3181 acctgcgcag tgccaataac gaagaaaaac agtcattcca gcttagcgac atccttgccg
     3241 ttttgcgtat cagcaccacc tggtattgca gcatggtgat ttttggtgtc ttcaccatct
     3301 acgccattct gagttactcc accaactatc tgaccgaaat gtacggcatg tcgctggtgg
     3361 cggcgagcta catggggatt gtgatcaaca aaatcttccg cgcgctgtgc ggcccacttg
     3421 gcggcattat caccacctac agtaaagtga aatcccctac ccgcgtgatc caaatccttt
     3481 ccatcatcgg tttgctggcg ttgacagccc tgctcgtcac gaactctaac ccgcaatcgg
     3541 tcgcgatggg gattggcctg attttactgc tgggattcac ctgttacgcc tcacgcgggc
     3601 tgtactgggc ctgccccggc gaagcgagaa caccgtctta cattatgggc accacggtag
     3661 gtatttgttc ggtgattgga ttcctgccgg atgtcttcgt ttacccgatt atcggccact
     3721 ggcaagacac cctgcccgct gcagaagcct accgcaatat gtggctgatg ggcatggcgg
     3781 cgctcgccat ggtgattgtc tttacctttt tgctgttcca aaaaattcgt actgctgata
     3841 gcgcccccgc aatggctagc agcaagtaag ccaggaaaat tacaggagaa aaatcatgtc
     3901 gaaaaaatac atcataggga ttgatggcgg aagtcagagc acaaaagtgg tgatgtacga
     3961 tctggaaggt aatgtggttt gcgaaggtaa aggcttatta cagccgatgc acacgcctta
     4021 tgccgatact gcagaacatc ctgacgacga tttatgggca tcattatgtt ttgcaggtca
     4081 cgatttgatg agccagtttg ccgggaacaa agaagatatt gttggcatcg gcctgggctc
     4141 catccgttgc tgccgtgcgt tattgaaagc cgacggcacg ccagctgcgc cgttgattag
     4201 ctggcaggat gcacgcgtta cacgccctta cgaacatacg aatcctgacg tggcgtatgt
     4261 cacctctttt tcgggttatc tgacgcatcg cttaaccggc gagtttaaag acaatatcgc
     4321 caactatttt ggtcagtggc cggtggatta taagagctgg gcatggagcg aagatgctgc
     4381 ggtaatggat aagtttaata tcccccgtca tatgctgttt gatgtgcaaa tgcctggcac
     4441 cgtcctcgga cttatcacac cacaagccgc actggcgaca catttcccgg caggactgcc
     4501 ggttgtttgt accaccagtg ataaaccggt agaagctctg ggggccggat tactggatga
     4561 tgaaactgcg gtaatttctc ttggcactta catcgcattg atgatgaacg gcaaagcact
     4621 gccgaaagat ccggtggcgt actggccgat tatgtcttct attccgcaaa cattgctgta
     4681 tgaaggttac ggtattcgca aaggtatgtg gacggtgagc tggctgcgtg acatgttaag
     4741 cgagtcgtta attcaggatg ccagggcgca ggatctttca ccggaagatt tactcaacaa
     4801 gaaagcctct agtgtgccac ctggctgtaa tgggctgatg acggtgctgg actggctgac
     4861 caatccgtgg gaaccgtaca aacgcgggat tatgatcggc tttgattcca gcatggatta
     4921 cgcatggata tatcgttcga tattggaaag cgtggcgcta acgctgaaga acaattacga
     4981 caatatgtgt aatgaaatga atcactttgc gaagcatgtg atcattactg gcggcggttc
     5041 gaacagcgat ctgtttatgc aaatttttgc cgacgtgttc aaccttccgg cacgccgtaa
     5101 cgccattaac ggttgtgcaa gtctgggagc agcgattaat acagcggtag gtctggggct
     5161 atacccggat tacgcaacgg ctgttgataa gatggttcgc gtgaaagata tctttatacc
     5221 gattgagagc aatgccaaac gctacgacgc gatgaataaa ggcattttca aagacctaac
     5281 caaacatact gatgtgatcc tgaaaaaatc gtatgaagtg atgcatgggg aattggggaa
     5341 tgcggattcg atccagagct ggtcgaatgc gtgaataagg taggccgggc ggaaaactgc
     5401 ccggcctgaa catacctgaa tggttatccc cgctgacgcg gggaacataa gttcgacaac
     5461 ttaaaaaatg ataacaagtc cggtttatcc ccgctgatgc agggaacata ataaaaactt
     5521 taactagttc ggtttatccc tgtttttaca aggaatatta actacactcg atgtctttag
     5581 aataaaagac aatacgaatc gcactttatc aattttcact taaagtatga actggatatc
     5641 tttattcata agaactattc atcaacagcg tatagaggcg gttatgtaaa accactcatt
     5701 agcctcaaaa caaagacaaa cttccagatt tattacttaa ccaatgaata acaagct
//
LOCUS       DAZGAN020000200         5666 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGAN020000200 DAZGAN020000000
VERSION     DAZGAN020000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.200"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            71..1621
                     /locus_tag="ACHKYO_RS24210"
                     /old_locus_tag="pgaptmp_004842"
     CDS             71..1621
                     /locus_tag="ACHKYO_RS24210"
                     /old_locus_tag="pgaptmp_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HGU4431528.1"
                     /translation="MSHQCSLSELNENLVPFTARQIKSSLIWCAEDVRNPGELQNACS
                     YIIDPDSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERH
                     SNGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGRKSRRALLV
                     REPVVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTD
                     PRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPGDSP
                     ADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEA
                     CYYCYLELLGIPGEFITQKEIPDILKELVNSFVALLNKYCEKSHAQDIVNQDGSPLQK
                     LVVTLIHHRHNQKQALNSSIKNDVYFTVAQQCFSQAIHWLTQGSTRRQINASLLLKEI
                     EHHTMKRLQPREELRKENMCEKIAILLENHGDDPLFLQEAISDMKNFMLKFSRDAFGY
                     LEPIRNTV"
     gene            1648..2100
                     /locus_tag="ACHKYO_RS24215"
                     /old_locus_tag="pgaptmp_004843"
     CDS             1648..2100
                     /locus_tag="ACHKYO_RS24215"
                     /old_locus_tag="pgaptmp_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HGU4431529.1"
                     /translation="MYYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            2253..3527
                     /locus_tag="ACHKYO_RS24220"
                     /old_locus_tag="pgaptmp_004844"
     CDS             2253..3527
                     /locus_tag="ACHKYO_RS24220"
                     /old_locus_tag="pgaptmp_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4431530.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGEPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVGNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGASVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            3520..5616
                     /gene="mchF"
                     /locus_tag="ACHKYO_RS24225"
                     /old_locus_tag="pgaptmp_004845"
     CDS             3520..5616
                     /gene="mchF"
                     /locus_tag="ACHKYO_RS24225"
                     /old_locus_tag="pgaptmp_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HGU4431531.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFQVETQQTRI
                     SLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISA
                     GLMFFILLRAAVSMLRAWSSLVMSTLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSR
                     FGSLDTLRATFTTCVVGAIMDSIMVVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTY
                     GYYRQISEETLVRGARASSYFMESLYGIATVKIQGMVGIRGTHWLNLKIDAINSGIKL
                     TRMDLLFGGINTFVAACDQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLT
                     SFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQV
                     FSGLNLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYH
                     SMIACVMQDDRLFSGSIRENICGFAEETDDEWMTECARASHIHDVIMKMPMGYETLIG
                     ELGEGLSGGQKQRIFIARALYRKPRILFMDEATSSLDTESERFVNAAIKKMNITRVII
                     AHRETTLRTVDRIISI"
BASE COUNT         1616 a         1067 c         1403 g         1580 t
ORIGIN      
        1 gctgtcagga cacttacttg ctcttttatg aacactcttt tatgaaaatt aaaacggaga
       61 atactgccag atgagtcatc agtgttcact ttctgaactg aatgaaaacc tggtgccttt
      121 cactgccagg cagatcaagt cctcattaat ctggtgtgca gaggatgtca gaaatccagg
      181 cgagctgcaa aatgcctgca gttatattat cgatcctgac agtacggctt ctgccaaagt
      241 gttccatgca gagcgctatg gtggcagtgg tattcagcgt aatggaggtg gtgcacgttg
      301 tgggtttgat ggtaactacc aggttaaagg aataggaagt aatccgttgg ttggcgaagg
      361 tactgacgaa cgtcattcta atggtgcact cggcgctgtt catgcaatat atgaggcttt
      421 gtggggagaa gtactggctc aaatattacc ttatagtgct gtgcgggttc gggcggtttt
      481 acttacagat ctctatactg aaaaggcatt tgagcgctcc ggtaggaaat cacgaagagc
      541 cctgttggta cgtgagcctg ttgttcgccc ggcgcatttt gaacgggcac cttactttca
      601 agtaaaaccg gagtattcca gtctattaat tcacgatgcc tgtcgggtta gatctgtgat
      661 ccacaagctg ccaggatatc tacctgtacc accggaagaa attgatgctg aagcacgaac
      721 tgatccccgg atttattgca ttgaggggtt atgtgaattg gcacgtcgtg aggcctggca
      781 aatggcattt tgtcgaacac gtttcctgag attgacaact tctccttcta atattgcaat
      841 ggatggcaga ttaatggatt ttaacggact cagttgtctg tttccgggag attccccagc
      901 tgattttggg tataaactaa gattagctga actggcaaaa gaaccgatgg tacttatgca
      961 agggctgtct gatctctgct tgtatatcgg aaaatatatg tttgaccctg acttcactct
     1021 tgcagcccgt ttgaaggttg aggagatatt tcagaaaact tttcatgaag catgttatta
     1081 ctgttatcta gaactgttgg gtattcctgg agaatttata acacaaaaag agatacctga
     1141 tatattgaaa gaactggtta acagttttgt tgcattactc aataaatact gtgagaaatc
     1201 acatgcccaa gatattgtca atcaggatgg ttcaccattg caaaagttgg ttgtgacgct
     1261 aatccatcat aggcataatc aaaagcaggc actgaatagt agcatcaaga atgatgttta
     1321 tttcacagtt gcacaacagt gtttttccca ggccatccac tggctgacgc aaggcagtac
     1381 cagacgtcag ataaatgcct cattactcct gaaagaaatt gaacatcata ccatgaaaag
     1441 gctgcaaccc agggaagagc tgaggaaaga gaatatgtgc gaaaaaattg ccatcctgct
     1501 ggagaatcat ggcgatgatc cccttttttt acaagaagca atttctgata tgaaaaattt
     1561 tatgcttaag ttttccagag atgcatttgg atatcttgaa ccgataagaa acacagtgta
     1621 ataaatatat aaagggaaat agtaatcatg tattatataa gggaaaccat cagaggaaaa
     1681 gatgaatgga ctgtttatga acagatcggt tttgcggtca gttgtatgct ctacaatcgt
     1741 aattacagtc tgtatccggt gttaaccatt caatactgga ctgaatatgc gatacagcat
     1801 aatcagatta aattcctgtt tgattcacga ggttttccac tggcgtatat tacctgggca
     1861 tatcttgagg ctgatacaga agcgcgcctg ctcagggatc cagaattcag gttgcatccg
     1921 tctgaatgga atgaagatgg aaggatctgg attctggatt tctgttgtaa accaggcttt
     1981 ggtcgaaaag ttattgacta tctcatacag cttcagccat ggggagaagg agaagtacga
     2041 tggttaagca ggcgaaagaa aattgtgaca tacatccctg agcggctgca taaaacgtag
     2101 tacctctgaa gatacagaga taattgtaaa ttacggggta aatgcatcgc tgatactatt
     2161 ttgacaggac tctgtatttt ctggctttga ttatagcatt tcggttaacc agtgaaggta
     2221 ttacaggaaa agtgtgagta agaggagcta ttttgtttcg tcaggatgct ttagaaaaca
     2281 gaaaaatgaa gtggcaggga cgggcaatat tacttcccgg aataccacta tggttaatca
     2341 tgctgggaag catagtgttt attacggcat ttctgatgtt catcattgtt ggtacctata
     2401 gccgccgtgt taatgtcagt ggtgaggtca caacctggcc aagagctgtc aatatatatt
     2461 caggtgtaca aggatttgtt gtcaggcagt ttgttcatga aggacagttg ataaaaaaag
     2521 gggagcctgt ttatctgatt gacatcagta aaagtacacg tagtggtatt gtcactgata
     2581 atcatcgtcg ggatatagaa aaccagctgg ttcgtgtggg caacattatt tcccgtctgg
     2641 aagaaagtaa aaaaataacg ctggataccc tggaaaaaca acgtctgcaa tacacagatg
     2701 cgtttcgtcg ctcatcagat attatacagc gtgcagagga agggataaaa ataatgaaaa
     2761 acaatatgga gaattacaga aactatcagg caaaagggct gattaataaa gatcagttaa
     2821 ctaaccaggt ggcattatat tatcagcaac aaaacaatct tctcagcctg agcggacaga
     2881 acgaacagaa tgccctgcag ataaccactc tggagagtca gattcagact caggctgcag
     2941 attttgataa ccgtatctac cagatggaac tgcaacggta cgagttacag aaagaactgg
     3001 ttaacactga tgtggagggc gaaatcatta tccgggcgtt gactgacggg aaagttgact
     3061 ccctgagtgt cactgtcggg caaatggtca ataccggaga cagccttctg caggttattc
     3121 cagagaacat tgaaaactat tatcttattc tctgggtccc gaatgatgct gttccttata
     3181 tttcggctgg tgacaaagtg aatattcgtt atgaagcctt tccggcagaa aaatttgggc
     3241 agttctctgc tacggttaaa actatatcca ggactcctgc gtcaacacag gaaatgttga
     3301 cctataaggg agcaccacag aatacgccgg gcgcctctgt tccctggtat aaagtcattg
     3361 cgatgcctga aaagcagatt atcagatatg acgaaaaata cctccctctg gaaaatggaa
     3421 tgaaagccga aagtacacta tttctggaaa aaaggcgtat ttaccagtgg atgctttctc
     3481 ctttctatga catgaaacac agtgcaacag gaccgctcaa tgactaacgg gaatttcaga
     3541 caaattataa atcagcttga tatgcgctgg cgacgtcgtg ttccggttat tcatcagacg
     3601 gagaccgctg aatgtggact ggcctgcctg gcaatgatat gcggtcattt tggtaagaat
     3661 attgacctga tatctcttcg ccggaagttt aatctctcgg cccgtggagc aaaccttgca
     3721 ggaatcaatg gaatagcgga gcaactgggg atggtcaccc gggctctttc actggagctg
     3781 gatgaacttg gtgccctcaa aatgccgtgt attctccact gggatttcag tcacttcgtc
     3841 gtgctggtca gcgtaaagcg taaccgttat gtactgcatg atccggccag aggcagaaga
     3901 tatctcggtc gggaggaaat gagccggtat tttacgggca ttgcacttga ggtctggccc
     3961 ggaagtgaat tccaggtgga aacccagcag acccgcataa gtctccgttc actgattaac
     4021 agtatttacg gtattaaaag aacactggcg aaaattttct gtctgtcagt tgtaattgaa
     4081 gcaatcaatc tggtaatgcc ggtgggaact cagctggtta tggatcatgc gattccggcg
     4141 ggggacagag ggctgctgac gcttatttct gctggcctga tgttctttat attgctcagg
     4201 gccgcggtga gtatgctgcg tgcatggtcc tcactggtta tgagcacgct catcaatata
     4261 cagtggcagt cgggtctgtt taaccatctt ctcagactgc cgctggcgtt ttttgaacgc
     4321 cgtaaattag gtgatatcca gtcgcgtttt ggctcccttg acactttgag ggccaccttt
     4381 accacctgtg tggttggggc aatcatggac agtattatgg ttgtaggtgt ttttgtgatg
     4441 atgctgttat atggaggata tcttacttgg atagttctcg gttttaccat ggtttacgtt
     4501 cttattcgtc tggtgacata cggctattac cggcaaatat cggaagaaac tcttgtcagg
     4561 ggggcccggg ccagctccta ttttatggaa agcctgtatg gtattgccac ggtaaaaatc
     4621 cagggtatgg tcgggatccg gggaacacac tggcttaacc tgaaaataga tgcgatcaat
     4681 tcaggtatta agttaactag gatggatttg ctcttcgggg gaataaatac ttttgttgcc
     4741 gcctgtgatc aggtggcgat tttatggctg ggtgcaagcc ttgtgatcga taatcagatg
     4801 acaataggga tgtttgtggc atttggttct tttcgtgggc agttttcgga tcgggttgct
     4861 tcgctgacca gttttcttct tcaactgaga ataatgagtc tacataatga gcgcattgca
     4921 gatattgcac tacatgaaaa ggaagaaaag aaaccggaaa ttgaaatcgt tgctgacatg
     4981 agcccggttt cactggaaac cactgattta agctaccggt atgacagcca gtcagcacag
     5041 gtattcagtg gtctgaattt gtctgtggct ccgggagaaa gtgtggctat aactggtgcc
     5101 tccggtgccg gaaaaaccac attaatgaaa gtattatgtg gactgtttga accagatagt
     5161 ggaaaagtac tggttaatgg cacggatata cgtcaacttg gaataaataa ttatcacagt
     5221 atgatagcct gtgttatgca ggacgaccgg ctattttcag gatcaattcg tgaaaatatc
     5281 tgtgggtttg cagaagaaac agacgacgaa tggatgacag aatgtgccag agcaagtcat
     5341 attcatgatg tgataatgaa aatgccaatg gggtatgaaa cgttaatagg tgaactgggg
     5401 gaaggtcttt ccggcggtca aaaacagcgt atattcattg cccgagcttt ataccggaaa
     5461 cctagaatat tatttatgga tgaggctaca agttctcttg atacagaaag tgaacgtttc
     5521 gtgaatgctg ccataaaaaa aatgaatatc acccgggtga ttattgcaca cagagaaact
     5581 acgttgagaa ctgttgacag gattatttct atttaaaatc cactggtgta actttgtaag
     5641 gagttttgtc gatgggggag gttaag
//
LOCUS       DAZGAN020000201         5660 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGAN020000201 DAZGAN020000000
VERSION     DAZGAN020000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.201"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..870
                     /locus_tag="ACHKYO_RS24230"
                     /old_locus_tag="pgaptmp_004846"
     CDS             <1..870
                     /locus_tag="ACHKYO_RS24230"
                     /old_locus_tag="pgaptmp_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4431532.1"
                     /translation="RYSSFASGAVPASSQPLADDPAVRAVFRHEAVIRRAGGVECLES
                     WLLREKGCQWPHSDWHSENMTTMRHAPGAIRLCWHCDNLLRDQFTERLEAMATDNCAR
                     WVLSVVRRDLGFDDSHVVTMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRE
                     SDLVPSVPATSIIQDKAKKVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCAC
                     CGKPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHDKLHADTVAFEEKYGSQLELIF
                     RFIDRALAIGVLS"
     gene            888..1280
                     /locus_tag="ACHKYO_RS24235"
                     /old_locus_tag="pgaptmp_004847"
     CDS             888..1280
                     /locus_tag="ACHKYO_RS24235"
                     /old_locus_tag="pgaptmp_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4431533.1"
                     /translation="MRDIQMVLERWGAWAANNHENVSWSPIAAGFKGLIPSKVKFRPQ
                     CCDDDGLIISSVMAVLKKKEPYQYQLLEMYYVYGITLRALGAKMGISLNQVVIRLQKA
                     EGFVEGCLAMLEVSLEMDCYMNHENDSR"
     gene            1347..1964
                     /locus_tag="ACHKYO_RS24240"
                     /old_locus_tag="pgaptmp_004848"
     CDS             1347..1964
                     /locus_tag="ACHKYO_RS24240"
                     /old_locus_tag="pgaptmp_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431534.1"
                     /translation="MNENVRHILLDAIENKKSLTVIYLGGSQPGTLRDISPISISEDK
                     LRARCHSTDAVKFFNIGKIQLPSDSSMITVPYGSLELKVYETMQSVYEDFHSLYPEGR
                     WGVEFNDHEFALFDFFKNGKRKKTAFMAIQFMERHEDETRTEITIDVSLSGVVISEGA
                     RTPKRRPWVVTGPGRGELRTYSTLDKAATEFFKRLSLMVSMAIET"
     gene            2198..2395
                     /locus_tag="ACHKYO_RS24245"
                     /old_locus_tag="pgaptmp_004849"
     CDS             2198..2395
                     /locus_tag="ACHKYO_RS24245"
                     /old_locus_tag="pgaptmp_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4431535.1"
                     /translation="MLKQQDMTETARVVFNELSATEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(2578..2715)
                     /locus_tag="ACHKYO_RS24250"
                     /old_locus_tag="pgaptmp_004850"
                     /pseudo
     CDS             complement(2578..2715)
                     /locus_tag="ACHKYO_RS24250"
                     /old_locus_tag="pgaptmp_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2967..4949
                     /locus_tag="ACHKYO_RS24255"
                     /old_locus_tag="pgaptmp_004851"
     CDS             2967..4949
                     /locus_tag="ACHKYO_RS24255"
                     /old_locus_tag="pgaptmp_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4431536.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSDGEGTVISTTSEPEYYFVVVLAGQSNSMSFGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDVSNLNHPKADLNKGQYGC
                     VGHALHVAKKLLPFMPARAGILLVPCGRGDSGFTAGAEGAFNEASGATAGSSLWGVDK
                     PLYRDLVSRTRAALKKNPKNVLLSVIWMQGEKDVSSGRHAEHNALFLAMVNKYRADLA
                     DIADQCTGGTTSGVPWICGDTTYDWKAKYAVQYEAVYGGYKGKAAQNIHFVPLMTDEH
                     GANVPTNEPSEDPDIITAGYYGAASRSNGNWTTADRKTHFSSWARRGIVSDRLAGEIL
                     VRAGRLLPFLSGQSAPLATTPASTGDAQSGSAGQTQQQGAGTSAGGHTEAVTRMVAGY
                     DANSGSGVWTEQQWNASGGKGTVTDDSGRKALRLEKQPGKLTSWKMFRTVAVEEAKNL
                     LSKGGEIAVRFKIPEGVELVNGQFVFGLYWPVSQWASGATANSMLASFFLQTDASNLN
                     LMYHKGESNAQLGTFGAFDHNWHTVVFRFAGNNSEKVVPVIDDAEQSAFDLVMWTNDG
                     FTADTLTLTDITGAKATYPVLLDTVTVKVNESRASS"
     gene            5089..5283
                     /locus_tag="ACHKYO_RS24260"
                     /old_locus_tag="pgaptmp_004852"
     CDS             5089..5283
                     /locus_tag="ACHKYO_RS24260"
                     /old_locus_tag="pgaptmp_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4431537.1"
                     /translation="MTFVHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGAKTEAPLIPEQPS"
     gene            5195..5599
                     /locus_tag="ACHKYO_RS24265"
                     /old_locus_tag="pgaptmp_004853"
     CDS             5195..5599
                     /locus_tag="ACHKYO_RS24265"
                     /old_locus_tag="pgaptmp_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4431538.1"
                     /translation="MIKGPQRKSAPVSQPEQKQKPHSFRNNLLNNPFQRENPMSEIKS
                     LVTAEAVKEVLRSEEVRSALKQQLRQNLEARLDAEVDSILDELLGAQPEPSPELLPEP
                     QAEDVTTENGDIQPEPPVTDMTDTQPESGTML"
BASE COUNT         1443 a         1212 c         1625 g         1380 t
ORIGIN      
        1 cgttattcat cttttgccag tggtgccgtt ccggcatcat cacaaccgct ggcggatgat
       61 cctgccgttc gggccgtgtt ccgccatgag gcggtgatcc gtcgtgctgg tggcgtggaa
      121 tgccttgaga gctggttact tcgtgaaaag ggctgtcagt ggcctcattc cgactggcac
      181 agcgagaaca tgaccacaat gcggcacgcg ccaggcgcaa tccgtctgtg ctggcactgt
      241 gacaatcttc tccgtgacca gttcacggaa cggctggaag caatggcaac ggataactgt
      301 gcccgctggg tgttgtctgt tgtgcgccgt gatcttggtt ttgatgacag tcacgttgtg
      361 acaatgccgg aactgtgctg gtggctggtt cgtaatgacc tggcggatgc tttaccggaa
      421 agtgcagccc gtaaggccct gagattaccg aagcctgttg tgccgtctgt cacccgggaa
      481 agtgaccttg tgccttcggt tcctgccacc agcatcatcc aggataaggc gaaaaaggtg
      541 ctggcgctga aagtggatcc ggagtcgccg gagtctttta tgttacgccc aaaacgtcgc
      601 cgctgggtta atgaaaagta cacgcgctgg gttaagacac agccgtgtgc atgttgtgga
      661 aagcctgctg atgatcccca ccacctgata ggccacggtc agggtggtat ggggacaaaa
      721 gcgcatgacc tttttgtgtt gccgttgtgc agaaaacacc atgacaaact gcatgcggat
      781 accgtggcat ttgaagagaa gtatggttcc caactggagc tgatatttcg ttttatcgat
      841 cgcgcgctgg cgattggtgt gctgtcctga ttttgtggag aaagttgatg cgtgatattc
      901 agatggttct cgaacgctgg ggagcatggg cggcaaataa tcatgagaat gttagttggt
      961 cgccaattgc tgcggggttt aaaggcctca tcccctcaaa agtaaaattt cgaccgcaat
     1021 gttgcgatga tgatggtctt atcattagtt cagttatggc tgttctcaag aaaaaggaac
     1081 catatcaata ccaattactg gaaatgtatt atgtatatgg gatcacatta cgagcgttag
     1141 gggcgaaaat ggggatatcg cttaatcaag ttgttattag attgcagaaa gctgaagggt
     1201 ttgttgaagg gtgtctggca atgctcgagg tatctttaga aatggattgc tatatgaatc
     1261 acgaaaatga ttcaagataa atgaacagtt actagttata ttttacagta atctcctgat
     1321 gatacacgtt cagcaggagg ttatttatga atgaaaatgt aagacacata ttgctggatg
     1381 caattgaaaa taagaaatct ttaacggtaa tttacttagg aggtagtcag ccaggaacat
     1441 tgagagatat atctccgata agcatatcag aagataaact tagggcaaga tgccatagta
     1501 ctgatgctgt aaagtttttt aatattggga aaatacaact acctagtgac tcgtccatga
     1561 taactgtgcc atatggaagt ttagagctta aagtttatga gacgatgcag agtgtgtatg
     1621 aggactttca tagcctgtat ccggaaggtc gatggggagt tgaatttaat gatcatgaat
     1681 ttgctctatt tgattttttc aaaaatggaa aaagaaaaaa aacagcattt atggcaattc
     1741 agtttatgga aagacatgag gatgaaacaa gaacggagat aacaattgat gtcagtctat
     1801 caggtgttgt gatttcggag ggagccagaa cacctaaaag acgtccgtgg gttgtgactg
     1861 gtcccgggcg tggagagcta agaacttatt caacgttaga caaagctgct acagagtttt
     1921 ttaagcgact ttcattgatg gtatctatgg cgattgaaac ataagggata taaagatacc
     1981 gaggaaatga tttgtgttat aacaaaaacc atcttaatct gttaagagtg gtcactccgt
     2041 catacagctt aaacctgccg cctggcgggt tttttatgcc caaaatcgcg tcagtacagt
     2101 aaacacgctg gtggttgcga atacgggtct ttcagcttgc tggctttttc gacaagagtt
     2161 attggtgtgt cacgttaacc ggaaaaggga aaaagacatg ctaaaacagc aggatatgac
     2221 agaaaccgcc agagtggtgt ttaatgaatt aagtgccacc gaaccggcga cagtcgggga
     2281 gattgcgcag aatacttacc tttcacgcga acgctgccag ttaatactga cccagctggt
     2341 tatggcgggt ctggcagact atcagttcgg ttgttacaga cgccttccgc agtgaaggct
     2401 tttttatttg tggtaaatgg gcggctggtg ggtgtggtgg ttgttgcttt cccgttgctg
     2461 aaaaagaaag catcaggcga ttagcagggt atcagttacc cgttgaaatt tttaaatacc
     2521 tcacaattca ggcggttgac tgttgtctgg tttgcgggga gtttgttaaa agaaactggc
     2581 atggtgaatc cccctgtgcg gaggggcaat cagcaactgg tgttttgtca ccgaccctta
     2641 tcctttctgt gcgggttcag gtgctgatac tgaactcacc gggaggcacc cggcaccatg
     2701 tgcatgatga tacagatacg cggctttagc ccctctccgg aggggctttc ttgtgggcaa
     2761 aaaaaagcct gagtgggttc gggcaacagc atgagatata catttttata atcgaatgga
     2821 ttttaaccag aattcataag gctgcgcaac tgcgtggcct ttttcgtatt gcgggctgta
     2881 gttttccttc tgccattgtc ctgtaacttc cggacttcag cccgcccctc atctgactca
     2941 caacattatc ccgaccggga ggattcatga catttaaaca ctacgatgtg gtcagggcgg
     3001 cgtcgccgtc agaccttgcg gagcgactga cacaaaaact gaaggagggg tggcagccat
     3061 ttggcagccc tgtcgccatc acgccttata ccctgatgca ggccattgcg gcggaaggtg
     3121 atgtcaccac acctgtggtg gtgaagccgt cggatggaga aggcacagtt atcagcacca
     3181 ccagcgaacc ggagtattac tttgttgttg ttctggcggg gcagtcaaac tcaatgtctt
     3241 ttggtgaagg gctgccgctg ccggagacat atgaccgtcc ggacccgcgt attaaacagc
     3301 tggcgcgtcg cagtacggtg acgccgggtg gtgccgcctg taaatataac gacatcattc
     3361 cggcagacca ttgtctgcat gatgtgcagg acgtgagtaa tctgaatcac ccgaaagcag
     3421 acctcaataa agggcagtat ggctgtgtgg ggcatgccct gcatgtggcc aaaaaactgc
     3481 tgccgtttat gcctgcccgt gcggggatcc ttcttgtccc gtgtggacgt ggcgattcgg
     3541 gatttactgc gggagcagag ggcgcgttta atgaggcgtc gggtgcgaca gcgggctctt
     3601 ccctgtgggg ggtggataaa ccgttgtatc gtgacctggt cagcagaacg cgtgcagccc
     3661 tgaagaaaaa tccgaaaaac gtgctgttgt cggtgatctg gatgcagggg gaaaaagatg
     3721 tcagttcggg gagacatgca gagcacaatg cactttttct tgccatggta aataaatacc
     3781 gtgcagacct ggcagatatt gcagaccagt gtactggcgg gacaacgtcc ggcgtcccgt
     3841 ggatttgcgg tgacaccacg tacgactgga aggcgaagta tgcagtgcag tatgaggcgg
     3901 tttacggagg ctataaaggc aaggcggcgc agaatattca ctttgtgccg ttgatgacgg
     3961 atgagcatgg tgcgaatgtg ccgacaaacg agccgtcaga agatccggac attatcacgg
     4021 cgggatacta tggtgccgcg tcacgcagta atggtaactg gacgacagcc gatcgtaaaa
     4081 cgcacttcag ctcctgggcg cgaagaggca ttgtttcaga tcggctggca ggagagatac
     4141 tggtgcgagc cgggcgtttg ctgccgttcc tgagcgggca gtctgcaccg ctggcgacca
     4201 cgccagcctc cacgggggat gcacagtctg gctctgcggg tcagacgcaa cagcagggtg
     4261 caggtacttc tgcaggcggt catactgaag ccgtaacaag aatggtggcc ggatatgatg
     4321 cgaacagtgg cagtggtgta tggacagagc agcagtggaa tgcgtccggt ggtaaaggca
     4381 ctgtgacgga tgacagtggc aggaaggcgc tgcgactgga aaaacagccg ggtaaactga
     4441 cctcctggaa gatgttccgt actgttgcgg tggaggaggc aaaaaatctt ctcagtaagg
     4501 gaggtgaaat tgccgtgcgg tttaagatcc cggagggtgt cgaactggtt aacggtcagt
     4561 ttgtctttgg tctgtactgg ccggtgtcgc agtgggcgtc aggcgcgaca gcaaacagca
     4621 tgctggcgtc cttcttcctt cagacggatg catcaaatct gaatctgatg taccacaagg
     4681 gggagtcgaa tgcgcaactg ggcacatttg gggcatttga ccataactgg catacagttg
     4741 ttttccgctt tgcgggaaat aacagcgaaa aagtggtgcc ggtgattgat gatgcagagc
     4801 agtctgcgtt tgacctggtg atgtggacaa atgatggctt tacagcagat acgctgacgc
     4861 tgacagatat cacgggggca aaagcgacgt atccggtact gcttgatacg gtcacagtca
     4921 aagttaacga aagccgggca tcatcataac cggcaaaaaa aaccgccagc ggcaggaacg
     4981 gaagctggcg gaggtaatcc caatggagaa tgtaaagaaa agatgctttc gtatatcggt
     5041 tttttaaatg aaaacagttc tcattgtcaa ccataacggt aagaaactat gacatttgtt
     5101 catcaggtga tgctgtactt ctgtacggct gtctgtgtgc tgtatcttct ttcgggtggg
     5161 tacagggcag tgcgcgattt ctggcgcagg cagattgata aaagggccgc agagaaaatc
     5221 agcgccagtc agtcagccgg agcaaaaaca gaagccccac tcattccgga acaaccttct
     5281 taataacccc tttcaacgag aaaatcctat gtcagaaata aaatcgctgg tcactgctga
     5341 ggcagtgaag gaagtcctgc gctctgaaga agtccggagc gcactgaaac agcaacttcg
     5401 gcagaacctt gaggcgcgtc ttgatgcaga agtggattca attctggatg aattgcttgg
     5461 tgcacagccg gaaccatccc cggaactgct tccggaacca caggcggaag atgtcaccac
     5521 ggaaaatggt gatattcagc cggagccacc ggtgacggat atgacagaca cacagccaga
     5581 atcgggcaca atgctgtaac ggcggggcag ggccatcagt aaagagctgg tggccctttt
     5641 gttgttgtga gcttccgagt
//
LOCUS       DAZGAN020000202         5659 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGAN020000202 DAZGAN020000000
VERSION     DAZGAN020000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.202"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            51..593
                     /gene="fimA"
                     /locus_tag="ACHKYO_RS24270"
                     /old_locus_tag="pgaptmp_004854"
     CDS             51..593
                     /gene="fimA"
                     /locus_tag="ACHKYO_RS24270"
                     /old_locus_tag="pgaptmp_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4431539.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRIDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            813..1505
                     /gene="fimC"
                     /locus_tag="ACHKYO_RS24275"
                     /old_locus_tag="pgaptmp_004855"
     CDS             813..1505
                     /gene="fimC"
                     /locus_tag="ACHKYO_RS24275"
                     /old_locus_tag="pgaptmp_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU4431540.1"
                     /translation="MMTKIKLLMLIIFYLIISASTHAAGGIALGATRIIYPADAQQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1536..4145
                     /gene="sfmD"
                     /locus_tag="ACHKYO_RS24280"
                     /old_locus_tag="pgaptmp_004856"
     CDS             1536..4145
                     /gene="sfmD"
                     /locus_tag="ACHKYO_RS24280"
                     /old_locus_tag="pgaptmp_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGU4431541.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMS
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDELIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4158..5165
                     /gene="fimH"
                     /locus_tag="ACHKYO_RS24285"
                     /old_locus_tag="pgaptmp_004857"
     CDS             4158..5165
                     /gene="fimH"
                     /locus_tag="ACHKYO_RS24285"
                     /old_locus_tag="pgaptmp_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU4431542.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            5176..>5659
                     /gene="sfmF"
                     /locus_tag="ACHKYO_RS24290"
                     /old_locus_tag="pgaptmp_004858"
     CDS             5176..>5659
                     /gene="sfmF"
                     /locus_tag="ACHKYO_RS24290"
                     /old_locus_tag="pgaptmp_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HGU4431543.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     K"
BASE COUNT         1481 a         1230 c         1370 g         1578 t
ORIGIN      
        1 atcggttata tacggatgtg gagtcgataa atgagattga aggaatatat atgaaattaa
       61 gatttatttc gtctgcgctg gctgccgcac tattcgccgc tacgggtagt tatgctgccg
      121 ttgtagatgg cggtacaatt cactttgaag gcgaactggt gaatgctgcc tgttcagtga
      181 atactgactc ggcagaccag gttgtcacac tcggtcaata tcgtatcgat attttcaatg
      241 ctgttggtaa tacctctgca ttaattccat tcaccattca gttgaacgac tgcgatcctg
      301 ttgttgccgc taatgctgcc gttgcatttt ctggtcaggc tgatgcaatc aatgataatt
      361 tattggccat tgcatccagt accaatacaa caacagcaac gggtgtcggt attgaaatac
      421 ttgataatac atccgcaatt ctcaaacctg atgggaatag cttctcaacc aaccagaact
      481 tgatccccgg gaccaacgtt cttcattttt ctgcacgtta taaaggcacc ggtacaagtg
      541 catcagcagg gcaagcaaat gctgacgcga cttttattat gagatacgaa taatcaaaac
      601 cacgttgttt tgaattatat atcacgtctt ataacaaagt aatgtaccgg ttgtctgaag
      661 cggtatggtg gcaatgtaaa tcgaaatcat gttcactttg tatcatgccg ctttattaaa
      721 tgaaaaggga atgatgtgtt gtaagaaacc aaagcaatca tttctttata tttcttattt
      781 ttgccgtcag gaatacacaa ggcgtattaa ctatgatgac taaaataaag ttattgatgc
      841 tcattatatt ttatttaatc atttcggcca gcacccatgc tgccggaggg atcgcattag
      901 gtgccacgcg tattatttat cccgctgatg ctcaacagac tgcggtatgg attagaaata
      961 gccataccaa tgagcgcttt ctggtcaatt cgtggattga aaacagcagc ggtgtaaaag
     1021 aaaagtcatt catcattaca ccgccactgt ttgttagtga acccaaaagc gaaaatacct
     1081 tgcgtattat ttacaccggt ccaccgctgg cggcagatcg tgagtctctg ttctggatga
     1141 atgttaagac gatcccttcg gtagataaaa atgcattgaa cggcaggaat gttttgcaac
     1201 tggcgatttt atcgcgcatg aaattatttc tccgtccaat tcaattacaa gaattacccg
     1261 cagaagcgcc ggacacactc aagttttcgc gatccggtaa ctatatcaat gttcataatc
     1321 cctcaccttt ttatgtcacc ctggttaact tacaagtggg cagccaaaag ttggggaatg
     1381 ctatggctgc acccagagtt aattcacaaa ttcccttacc ctcaggagtg cagggaaagc
     1441 tgaaatttca gaccgttaat gattatggtt cagtaacccc ggtcagagaa gtgaacttaa
     1501 actaaccgaa tcatctgaca atatcagagc taattatgaa aatacccact actacggata
     1561 ttccgcagag gtatacctgg tgtctggccg gaatttgtta ttcatctctt gccattttac
     1621 cctccttttt aagctatgcg gaaagttatt tcaacccggc atttttatta gagaatggca
     1681 catccgttgc tgatttatcg cgctttgaga gaggcaatca tcaacctgcg ggagtgtatc
     1741 gggtggatct ctggcgtaat gatgagttca ttggttcaca ggatatcgta tttgaatcga
     1801 caacagaaaa tacaggtgat aaatcaggtg ggttaatgtc ctgttttaac caggtactcc
     1861 ttgaacgaat tggccttaat agcagtgcat ttcccgagtt agcccagcag caaaacaata
     1921 aatgcatcaa tttactgaaa gctgtacctg atgccacaat taactttgat tttgcagcga
     1981 tgcgcctgaa catcactatt cctcagatag cgttgttgag tagcgctcac ggttacattc
     2041 cgcctgaaga gtgggatgaa ggtattcctg ctttactcct gaattataat ttcaccggta
     2101 acagaggtaa tggtaacgat agctattttt ttagtgagct cagcgggatt aatattggcc
     2161 cgtggcgttt acgcaacaat ggttcctgga actattttcg cggaaatgga tatcattcag
     2221 aacagtggaa taatattggc acctgggtac agcgcgccat tattccgctg aaaagtgaac
     2281 tggtaatggg agacggcaat acaggaagtg atattttcga tggtgttgga tttcgtggta
     2341 tacggcttta ctcttccgat aatatgtatc ctgatagcca gcaagggttt gccccaacgg
     2401 tacgtgggat tgcccgtacg gcggcccagc taacgattcg gcaaaatggt tttattatct
     2461 atcaaagcta tgtttccccc ggcgcttttg aaattacaga tttgcacccg acatcttcaa
     2521 atggcgatct ggatgtcacc atcgacgagc gcgatggcaa tcagcagaat tacacaattc
     2581 cgtattcaac agtgccgatt ttacaacgcg aagggcgttt caaatttgac ctgacggcgg
     2641 gcgattttcg tagcggtaat agtcagcaat catcaccttt cttttttcag ggcacggcac
     2701 tcggcggttt accacaggaa tttactgcct acggcgggac gcaattatct gccaattaca
     2761 ccgccttttt attaggactg gggcgcaatc tcggaaactg gggggcagtg tcgctggatg
     2821 tgacccatgc gcgcagtcag ttagccgacg acagtcgtca tgagggggat tccattcgct
     2881 tcctctatgc gaaatcgatg aacaccttcg gcaccaattt tcagttaatg ggttaccgct
     2941 attcgacaca aggtttttat acccttgatg atgttgcgta tcgtcgaatg gaggggtacg
     3001 aatatgatta cgattacgac ggtgagcatc gggatgaact gataatcgtg aattaccaca
     3061 atttacgctt tagccgtaaa gaccgtttgc agttaaatat ttcacaatca cttaatgact
     3121 ttggctcgct ttatatctct ggtacccatc aaaaatactg gaatacttcg gattcagata
     3181 cctggtatca ggtggggtat accagcagct gggttggcat cagttattcg ctctcatttt
     3241 cgtggaatga atctgtaggg atccccgata acgaacgtat tgtcggactt aatgtttcag
     3301 tgcctttcaa tgttctgacc aaacgtcgct acacccggga aaatgcgctc gaccgcgctt
     3361 atgcctcctt taacgccaac cgtaacagca acgggcaaaa tagctggctg gcaggtgtag
     3421 gtgggacctt actagaaggc cacaacctga gttatcacgt aagccagggt gatacctcga
     3481 ataatgggta tacgggcagc gccacggcaa actggcaggc cgcttacggt acgctggggg
     3541 tcgggtataa ctacgaccgc gatcaacatg acgttaactg gcagctgtct ggcggtgtgg
     3601 tcgggcacga aaatggcata acgctgagcc agcctttagg ggataccaat gttttgatta
     3661 aagcgcctgg cgcaggcggt gtacgcattg aaaatcaaac tggcatttta accgactggc
     3721 gcggctatgc ggtgatgccg tatgccacgg tttatcggta taaccgtatc gcgcttgata
     3781 ccaatacgat ggggaattcc atcgatgttg aaaaaaatat tagcagcgtt gtgccgacgc
     3841 aaggcgcgtt ggttcgtgcc aattttgata cccgcatagg cgtgcgggcg ctcattaccg
     3901 ttacccaggg cggaaaaccg gtgccgtttg gatcactggt acgggaaaac agtaccggaa
     3961 taaccagtat ggtgggtgat gacgggcaag tttatttaag tggtgcgcca ttgtctggtg
     4021 aattactggt tcagtgggga gacggcgcga actcacgctg cattgcgcac tatgtattgc
     4081 cgaagcaaag cttacagcaa gccgtcactg ttatttcggc agtttgcaca catcctggct
     4141 cataaaggaa attatcaatg aagataatct gcagattatt attggcgatg gcatgtttgt
     4201 gtctggcaaa catatcctgg gctactgttt gtgcaaatag tactggcgta gcagaagatg
     4261 aacactatga tctctcaaat atctttaata gcaccaataa ccagccaggg cagattgttg
     4321 ttttaccgga aaaatccggc tgggtaggtg tgtcagcaat ttgtccaccc ggcacgctgg
     4381 tgaattatac ataccgtagt tatgtcacca actttattgt tcaggaaact atcgataatt
     4441 ataaatatat gcaattaaat gattatctat taggtgcgat gagtctggtt gatagtgtga
     4501 tggatattca gttccccccg caaaattata ttcggatggg aacagatcct aacgtttcac
     4561 aaaaccttcc attcggggtg atggattctc gtttaatatt tcgtttaaag gttattcgtc
     4621 cctttattaa catggtggag atccccagac aggtgatgtt taccgtgtat gtgacatcaa
     4681 cgccttacga tccgttggtt acacctgttt ataccattag ttttggtggc cgggttgaag
     4741 taccgcaaaa ctgcgaatta aatgccgggc agattgttga atttgatttt ggtgatatcg
     4801 gcgcatcgtt atttagtgcg gcagggccgg gtaatcgacc tgctggtgtc atgccgcaaa
     4861 ccaagagcat tgcggtcaaa tgtacgaatg ttgctgcgca ggcttattta acaatgcgtc
     4921 tggaagccag tgccgtttct ggtcaggcga tggtgtcgga caatcaggat ttaggtttta
     4981 ttgtcgccga tcagaacgat acgccgatca cgcctaacga tctcaatagc gttattcctt
     5041 tccgtctgga tgcagctgcg gcagccaatg tcacacttcg cgcctggcct atcagtatta
     5101 ccggtcaaaa accgaccgaa gggccgttta gcgcgctggg gtatttacgc gtcgattatc
     5161 aatgaggtac ggagaatgag aagagtactc tttagctgtt tctgcgggtt actgtggagt
     5221 tccagtggat gggcagctga ccctttggga acgattaata tcaatttgca cggtaacgtt
     5281 gttgatttct cctgtaccgt aaacactgcg gatattgata agacggtaga tttaggcaga
     5341 tggcctacga cacaattact gaacgctggc gataccacgg cactcgtccc ttttagcctg
     5401 cggctggagg gatgtcctcc gggttcagtt gcgattttat ttacgggaac gccggcatcc
     5461 gataccaacc tgctggctct ggatgatccc gcaatggcgc aaaccgtcgc catcgaatta
     5521 cgtaatagcg atcgctcccg gctcgcactg ggagcggcga gcccgactga ggaagtagat
     5581 gcaaatggca atgtcacact aaactttttt gccaattatc gagcgttagc cagcggtgtt
     5641 cggccaggtg tggcgaaag
//
LOCUS       DAZGAN020000203         5537 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGAN020000203 DAZGAN020000000
VERSION     DAZGAN020000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.203"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            198..1160
                     /locus_tag="ACHKYO_RS24295"
                     /old_locus_tag="pgaptmp_004859"
     CDS             198..1160
                     /locus_tag="ACHKYO_RS24295"
                     /old_locus_tag="pgaptmp_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HGU4431544.1"
                     /translation="MSELVVYKANELAVSRYDLTEHETKLILCCVALLNPTLDKPTRE
                     DRTITFTYQQYAEMMGLSPDNAYHRLHKATSELMTRTVEIIYPTGDISKRIFQWVNYA
                     EFNRKTQSLTLVFSEDIIPYLFQLKKFIKYNLDYVKAFENKYSMRIYEWLLKELTQRK
                     THRANIEISIAEFKFMLLLEKHYPEFKELNRWVLKPITKDLNTHSNMKLAIGKRGRPA
                     DTLIFQVVLNQPVEPGDNGKNAPKNTTRTPIPALNMTEDELLYRALVNVLHRALIAKI
                     QLTKFDAKFLCDMQSKYHLNASFSWLTDKQREKLEKTLSKYRAF"
     gene            complement(1201..1938)
                     /locus_tag="ACHKYO_RS24300"
                     /old_locus_tag="pgaptmp_004860"
     CDS             complement(1201..1938)
                     /locus_tag="ACHKYO_RS24300"
                     /old_locus_tag="pgaptmp_004860"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HGU4431545.1"
                     /translation="MLYSTRDAIVERNAKKRRDTLTFLAEETCSTSSVIALLLNIKSV
                     PRANDFLRSLEKQEIVCCSDTFIAGRKVRLWGLTEHGAALSGREKVQPFHPSRVSPTQ
                     LQHRLDIQRIRLMLQSHPAVSDWRYPSQRRKGERSHDAEFRLNERIIGLEVERTVKSR
                     SRYEQIVSDFFVRIMRTKEDERANVPQYCWYVCPTEEIALRVGDALKRCTRINNNGKW
                     ESLPEAIIAEQRFKAFTYDAVEKWAKK"
     gene            complement(1938..3866)
                     /locus_tag="ACHKYO_RS24305"
                     /old_locus_tag="pgaptmp_004861"
     CDS             complement(1938..3866)
                     /locus_tag="ACHKYO_RS24305"
                     /old_locus_tag="pgaptmp_004861"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HGU4431546.1"
                     /translation="MDYIYFAYFSLKNDSTVAYIIASVIAFILGWISSSGFWCKQFVL
                     LGIPKSMIAARKKEWTGQNSTPGLAFFIDCDSPADEVLKIRNGYYNRYRLAWCSLAVF
                     LVILAGFLVLEIYTFGLFQTLDKISALNFDRFYLQTVIFFILGGIIGFVHIQSKKPNN
                     KSRELARINDIQDKARRKAAQRGNSLTDIRDVSFAAPAKFSPLEQMDKFSPGDRVFLG
                     FNGSAYKENSPISVTRRFWNTANIQIIGAPGSGKTKMATNCILQCVRDFGDSCLFFDP
                     KNDEHAPQVLHNNLPAGKFVKLDLRAGTPPQINPFGGLSYTDLKNLIINCFGMQESGK
                     ESDFYKLMQQDAANAVSKEFHGNANIFSLIDAFHRLDEKTKEPAKAFISRLESLAELS
                     CIHTESGFSLENAIEEGMCVYIVGSTSDLNVRFLQQILFARCIQNVMKREANREYRQL
                     LIMVDEIRYCLSPYVVDSLGTVRSKKCHLLLAHQSEGDFAAVQGKDFQSVRTAIMDNI
                     SLRWIYRATNAESAERASKETGLIRATTADRKALAVENNEEVMSGDVNIRETQRYAFD
                     ANTMLSLPEGMAVVVGLPKEKNNARSELIYTHHIEYSPSTIALPEAPALVRQTLDTLL
                     QPAPKAEPHSPLSAEDFR"
     gene            complement(3869..4267)
                     /locus_tag="ACHKYO_RS24310"
                     /old_locus_tag="pgaptmp_004862"
     CDS             complement(3869..4267)
                     /locus_tag="ACHKYO_RS24310"
                     /old_locus_tag="pgaptmp_004862"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4431547.1"
                     /translation="MKALRRLLGFSLIDMAKHFNVSPSTYHAYETGKYKAPDNVIQYF
                     WLLHNDVKVLYDDLRYLANNNRASISEQYGRYSEEATHCRIIATSYRSFEAFQKQFKD
                     KKPTYLEYRIREAAILLAAFSGDCEIENVG"
BASE COUNT         1491 a         1238 c         1340 g         1468 t
ORIGIN      
        1 aaaaaaaaca aagggttacg aggcaaaagg tgtagtaaaa atcataaagg gtgtagtaaa
       61 aatcacaaat aagtcatcga acggtgtagt aaaaatcaca gtattcacag gccatcacta
      121 aaaagtggtg cttgatcgcc atcctacgct tatgtatgtt ggtgtagcta aatattatat
      181 caacaccaag gtgatttatg tccgaactgg ttgtatacaa agcgaacgaa ctggccgtca
      241 gtcgttatga cctgacggag catgaaacca agttaattct gtgctgtgtg gcgctactca
      301 atccaacact ggataaaccc acccgcgaag acaggacaat tacattcacg tatcagcaat
      361 atgccgagat gatggggtta tctcctgata acgcgtatca cagattacac aaagcaacca
      421 gtgagttaat gaccagaacg gtagaaataa tctaccctac aggagatatt tctaaacgta
      481 tatttcaatg ggttaattac gctgaattta atagaaaaac ccaatcatta actttggttt
      541 ttagtgaaga cattatccct tacctgttcc agttaaagaa attcattaaa tataatctgg
      601 attatgtaaa agcgttcgaa aacaaatact cgatgcgcat atacgaatgg cttttaaaag
      661 aattaacaca gcgcaaaact cacagagcca atattgaaat cagtatcgct gaatttaaat
      721 tcatgttgtt actggaaaaa cattaccctg aatttaaaga actaaaccgt tgggtgttaa
      781 aaccaataac aaaagacctt aacacgcaca gtaacatgaa gttagctatt ggtaaacgtg
      841 gtcgccctgc ggatacactg atatttcagg tggtgctgaa tcagccagtc gaaccgggcg
      901 ataacggtaa aaatgctccg aaaaatacaa caagaacacc gatccctgct ctcaatatga
      961 cagaggatga actgttatat agggcactgg taaacgtctt gcacagggcg ctaatcgcta
     1021 aaatccagtt gacgaaattt gatgcgaaat ttttatgtga tatgcaaagc aaatatcatc
     1081 ttaatgcttc tttttcatgg ctgaccgata aacaaagaga aaaactcgaa aaaacgttga
     1141 gcaaatatcg cgccttttaa cacctaccct ggaaacaaag gcggctcacg ccgcctttat
     1201 ttattttttt gcccactttt ccacggcatc ataggtgaat gccttaaaac gttgctctgc
     1261 aataattgct tccggcagtg attcccactt cccgttgttg ttaattctgg tgcacctttt
     1321 caatgcgtcc cccacgcgta atgcgatttc ttccgtaggg cagacatacc agcagtactg
     1381 cggtacgttc gcgcgttcgt cttcctttgt ccgcatgatt cggacaaaaa aatctgaaac
     1441 gatttgctcg taccggctgc ggcttttcac ggttcgttca acttccagcc cgatgattcg
     1501 ctcgtttaat cgaaactcgg catcgtgtga ccgctcacct tttcgtcttt gcgagggata
     1561 acgccagtca gacacggcag gatgcgactg gagcatcagc cggatacgct gaatatcaag
     1621 gcggtgttgc agttgcgtcg gggacactct ggaagggtgg aatggttgga ctttctcccg
     1681 cccagaaagt gccgcaccat gttccgttaa cccccaaagg cgtactttgc ggccagcaat
     1741 gaaggtatcg gaacagcaga caatttcctg tttctccaga gagcgcagaa aatcgttggc
     1801 gcggggcact gatttgatat taagcaggag agcaatcact gacgacgtgc tgcacgtttc
     1861 ttctgccaga aaagtcagag tatcgcgccg ttttttggcg ttccgctcga caattgcatc
     1921 acgggtagaa tacagcatta gcggaagtcc tctgcgctta ggggtgagtg cggctctgct
     1981 tttggtgctg gctgtagcag agtatccaga gtctgtcgga ccagagccgg agcttccgga
     2041 agggcaatgg ttgatggcga gtattcaatg tgatgcgtgt agatcaactc cgagcgggca
     2101 ttgttttttt cttttgggag accaacaact accgccatgc cttccggtaa cgagagcatg
     2161 gtattggcat caaatgcgta acgctgtgtt tcgcggatat taacatctcc ggacatcact
     2221 tcttcgttgt tttcaactgc aagggctttg cgatctgctg ttgtcgcacg aatcaggccg
     2281 gtttcctttg aggcgcgttc ggcagattcg gcatttgtag ccctgtagat ccagcgcaga
     2341 gaaatgttgt ccatgatagc ggttctgacg gactggaaat cttttccctg gacggctgca
     2401 aaatcgcctt cggactggtg agcaaggagc agatgacatt ttttgctgcg gacggttccc
     2461 agtgaatcaa cgacgtaggg cgacaggcaa taacggattt catcaaccat gataagtagc
     2521 tgacggtatt cccgattcgc ttcgcgcttc atgacgtttt gtatacagcg cgcgaaaagt
     2581 atttgttgca gaaaacgaac gttgaggtcg ctcgtggagc cgacgatata aacgcacatg
     2641 ccttcttcga tcgcattctc cagggagaat ccagactccg tgtgaatgca ggataattcc
     2701 gcaagggatt ccagcctgga gatgaatgct tttgcaggtt ctttggtctt ttcatcgagg
     2761 cgatggaaag catctatcag ggaaaaaatg ttggcgttac cgtgaaactc tttcgacacg
     2821 gcattggcgg catcctgttg catgagcttg tagaaatccg attcttttcc gctttcctgc
     2881 atcccgaaac aattgatgat caggttcttc aaatcggtgt atgagagtcc gccgaacggg
     2941 ttgatctgtg gcggagtacc agcacggaga tcgagcttaa cgaattttcc ggcaggcaga
     3001 ttgttgtgta acacctgtgg ggcatgctca tcatttttgg ggtcaaaaaa cagacatgaa
     3061 tcaccgaaat cgcgcacgca ttgcaggatg cagttggtcg ccattttcgt tttgccgcta
     3121 ccaggtgcgc cgatgatttg aatgttggcg gtattccaga aacgacgggt gacagatata
     3181 ggcgagtttt ctttgtacgc agagccgtta aagccgagaa aaacccggtc gccaggcgaa
     3241 aatttatcca tttgttcgag cggggaaaac tttgcaggcg ctgcaaacga aacatccctg
     3301 atatctgtca gagaattgcc gcgctgggct gcttttcgtc ttgctttgtc ctgtatatcg
     3361 tttattcgtg cgagttcacg agatttgtta ttcggttttt ttgattgaat gtgaacaaat
     3421 ccaataatcc ccccgagaat aaagaaaatt accgtctgta gataaaaacg gtcgaagttc
     3481 agtgcgctga ttttgtccag tgtctggaat aaaccgaaag tgtatatttc aagtaccaga
     3541 aatcctgcaa gaatcacgag aaaaacagcc agtgaacacc aggcaagacg ataccgattg
     3601 taataaccat ttcgaatctt caacacctca tcagcaggag agtcgcaatc aatgaaaaat
     3661 gccagtccgg gcgtgctgtt ttgtcccgtc cattctttct tcctggcggc tatcatagat
     3721 tttggtattc caagaaggac aaactgttta caccagaatc cagaggaaga aatccatccc
     3781 aaaatgaaag caataactga agcgataatg tatgcaaccg ttgaatcatt tttcagagaa
     3841 aaatacgcaa agtaaatata atccataatc accctacatt ctctatttca cagtcaccgc
     3901 taaatgcagc taacagtata gctgcctccc ttattcgata ctccaaataa gtgggttttt
     3961 tgtctttgaa ttgtttttga aacgcttcaa acgagcggta acttgtagca atgatgcgac
     4021 agtgcgtagc ctcttcagaa tatctgccgt actgctcact aattgatgcc cggttattat
     4081 tagcgagata acgtaggtca tcatataaaa ctttaacgtc attgtgcaga agccaaaaat
     4141 actgaataac attatctggc gctttgtatt ttcccgtctc ataggcatga taagtacttg
     4201 gcgaaacatt gaagtgcttt gccatgtcta ttagcgaaaa gcccaacaat ctgcgcaatg
     4261 ctttcatggt gttctgtgct cccgagggat aaagtgaaca cttatacggt tccccgtatt
     4321 tccttgataa catgctatgg ctggtagccc caatataaca atcgtttgag atacggacga
     4381 catcttttcg aattttcata ttttccaaaa aaataacaag aagacgacgt tgatatcttt
     4441 cactattggt cggagtcgtc aagcagaaaa gcgaattttt ttcgcatacc tcgttcaaac
     4501 cactaggtag aaaaacggtg ctcagaattt ggcaggcggt gcagttgtgc gggaacgctg
     4561 acccaagaca gcgttagcga gccagccgcc ccagcggatt gctcgcgtcc cgtctttcga
     4621 attgcgtcca gcatttcgaa agatgcaagc gtctttcttc cccagaagaa aacgcgcagc
     4681 acacagagaa ggcgaacccg ccccagcggg tgagaaaaat cgacgctccc cagagcgttg
     4741 attttgatcc ttctcgccgt tcgtcaccgc aagtgaaacg acagtgaaac gctgcgtgac
     4801 gaacggcagg tgaatttctg gtgttgtcgg cgtccagcag acaaaagcca gaaagagggg
     4861 aaacgcagta attttggggg cgtccagccc gcgaaattac tgggggttcc ccttgtagtt
     4921 cgtccgccct agcggatgaa ctacacaggg cgacagccct gcatataggg atcatagacg
     4981 gggatcacat atggggatcg tatacggggc tagcagatac cccgatgtat gatccccata
     5041 tggtgttttt ttaatctcat tactttggcg gtcaaggaat gtggtttttt gttttttgcc
     5101 actgtggatt catatctcat atgcccttaa aacgcgctga gagcgattct ggcgcgtttt
     5161 ttttgggtag gtggtaggtt gggtcgcctg atgggcgttt aaggcgtctg aggctcgcca
     5221 gcgccgaata agttggggtt ggggtttgcg tttggagaaa ctgcccgccg tagccctagc
     5281 gaccgagcgt tagcgagcga gtgggcggag ggggcggaat agagctgtgg cgaagtttgg
     5341 cacttacgcg tcgatgttga atattgcgga gggggtcagg gggatacccc cagacggttt
     5401 ccggtggcgc tcaaggcgct accggaaccg aggcggcaca gtttcgaatc gtcgattttg
     5461 ccaggttatc cacaggcggc ggatctattc tcttttagat cttttattct ctttttattc
     5521 tagggcttcg cgttttc
//
LOCUS       DAZGAN020000204         5511 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGAN020000204 DAZGAN020000000
VERSION     DAZGAN020000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.204"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1442
                     /locus_tag="ACHKYO_RS24315"
                     /old_locus_tag="pgaptmp_004863"
     CDS             <1..1442
                     /locus_tag="ACHKYO_RS24315"
                     /old_locus_tag="pgaptmp_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4431548.1"
                     /translation="AAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVW
                     PKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTP
                     DDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSV
                     TFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAE
                     RKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTL
                     QRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYG
                     KPVANMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDL
                     TEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSAL
                     LASLQEEDGAATNKKTLADYARALSGEDE"
     gene            1439..1645
                     /locus_tag="ACHKYO_RS24320"
                     /old_locus_tag="pgaptmp_004864"
     CDS             1439..1645
                     /locus_tag="ACHKYO_RS24320"
                     /old_locus_tag="pgaptmp_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HGU4431549.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            1642..3243
                     /locus_tag="ACHKYO_RS24325"
                     /old_locus_tag="pgaptmp_004865"
     CDS             1642..3243
                     /locus_tag="ACHKYO_RS24325"
                     /old_locus_tag="pgaptmp_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4431550.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRDKLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            3224..4543
                     /locus_tag="ACHKYO_RS24330"
                     /old_locus_tag="pgaptmp_004866"
     CDS             3224..4543
                     /locus_tag="ACHKYO_RS24330"
                     /old_locus_tag="pgaptmp_004866"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4431551.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEAPATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQVT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            4553..4885
                     /locus_tag="ACHKYO_RS24335"
                     /old_locus_tag="pgaptmp_004867"
     CDS             4553..4885
                     /locus_tag="ACHKYO_RS24335"
                     /old_locus_tag="pgaptmp_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU4431552.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVDADQTSTTLTYYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            4941..>5511
                     /locus_tag="ACHKYO_RS24340"
                     /old_locus_tag="pgaptmp_004868"
     CDS             4941..>5511
                     /locus_tag="ACHKYO_RS24340"
                     /old_locus_tag="pgaptmp_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4431553.1"
                     /translation="MSMYTTAQLLAANEKKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALN"
BASE COUNT         1250 a         1471 c         1690 g         1100 t
ORIGIN      
        1 aagcggcaaa aaactaccgt gaaaagtcgg tggatgtggc gggttatgat gaacttgctg
       61 cctttgatga ggatattgaa caggaaggct ctccgacgtt cctgggcgat aagcgtattg
      121 aaggctcggt ctggccaaag tccatccgtg gctccacgcc caaagtgaga ggcacctgcc
      181 agattgagcg tgcagccagt gaatccccgc attttatgcg ttttcatgtt gcctgcccgc
      241 actgcgggga ggagcagtac cttaaatttg gcgataaaga gacgccgttt ggcctcaaat
      301 ggacgccgga tgacccctcc agcgtgtttt atctctgcga gcataacgcc tgcgtcatcc
      361 gtcagcagga gctggacttc actgatgccc gttatatctg cgaaaagacc gggatctgga
      421 cccgtgatgg cattctctgg ttttcgtcat ccggtgaaga gattgaaccg cctgacagcg
      481 tgacctttca catctggacg gcgtacagcc cgttcaccac ctgggtgcag attgtcaaag
      541 actggatgaa gacgaaaggg gatacgggaa aacgtaaaac cttcgtgaac accacgctcg
      601 gtgagacgtg ggaagcgaaa atcggtgaac gtccggatgc tgaagtgatg gcagagcgga
      661 aagagcatta ttcagcgccc gttcctgacc gtgtggctta cctgaccgcc ggtatcgact
      721 cccagctgga tcgctacgaa atgcgcgttt ggggatgggg gccgggtgag gaaagctggc
      781 tgattgaccg gcagattatt atgggccgcc acgacgatga acagacgctg cagcgtgtgg
      841 atgaggccat caataaaacc tatacccgcc ggaatggtgc agaaatgtcg gtatcccgta
      901 tctgctggga tactggcggg attgacccta ccattgtgta tgaacgctcg aaaaaacatg
      961 ggctgttccg ggtgatcccc attaaagggg catccgtcta cggtaagccg gtggccaaca
     1021 tgccacgtaa gcgaaacaaa aacggggttt accttaccga aatcggtacg gataccgcga
     1081 aagagcagat ttataaccgc ttcacactga cgccggaagg ggatgaaccg cttcccggtg
     1141 ccgttcactt cccgaataac ccggatattt ttgatctgac cgaagcgcag cagctgactg
     1201 ctgaagagca ggtcgaaaaa tgggtggatg gcagaaaaaa aatactgtgg gacagcaaaa
     1261 agcgacgcaa tgaggcgctc gactgcttcg tttatgcgct ggcggcgctg cgcatcagta
     1321 tttcccgctg gcagctggat ctcagtgcac tgctggcgag cctgcaggaa gaggatggtg
     1381 cagcaaccaa caagaaaaca ctggcagatt acgcccgtgc cttatccgga gaggatgaat
     1441 gacgcgacag gaagaacttg ccgctgcccg tgcggcactg catgacctga tgacaggtaa
     1501 acgggtggca acggtacaga aagacggacg gcgagtggag tttacggcca cttccgtgtc
     1561 tgacctgaaa aaatacattg cggagctgga agtgcagacc ggcatgacac agcgacgcag
     1621 gggacctgca ggattttatg tatgaaaacg tccaccattc ccacccttct ggggccggac
     1681 ggcatgacat cgctgcgtga atatgccggt tatcacggcg gtggcagcgg atttggtggg
     1741 cagttgcggg cgtggaaccc accgggtgaa agtgtggatg cagccctgct gcccaatttt
     1801 acccgtggca atgcccgtgc agacgatctg gtacgcaata acggctatgc cgccaacgcc
     1861 atccagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca tcgcccaagc
     1921 tggcgctatc tgggcatcgg ggaggaagac gcccgtgcct tttcccgcga ggttgaagcg
     1981 gcatggaaag agtttgccga agatgactgt tgctgcattg acgttgagcg aaaacgcacg
     2041 tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga gctgttcgtt
     2101 caggccacct gggataccag ttcgtcgcgg cttttccgga cacagttccg gatggtcagc
     2161 ccgaagcgca tcagcaaccc gaacaatacc ggagacagcc ggaactgccg tgccggtgtg
     2221 cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg gtatcctggc
     2281 tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg cgcctcgttc
     2341 attcacgttt ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc
     2401 gtgatggagc agatgaagat gctcgacacg ctgcagaaca cgcagctgca gagcgccatt
     2461 gtgaaggcga tgtatgccgc caccattgag agtgagctgg atacgcagtc agcgatggat
     2521 tttattctgg gcgcgaacag tcaggagcag cgggacaagc tgaccggctg gattggtgaa
     2581 attgccgcgt attacgccgc agcaccggtc cggctgggag gcgcaaaagt gccgcacctg
     2641 atgccgggtg actcactgaa cctgcagacg gctcaggata cggataacgg ctactccgtg
     2701 tttgagcagt cactgttgcg gtatatcgct gccgggctgg gtgtctcgta tgagcagctt
     2761 tcccggaatt acgcccagat gagctactcc acggcacggg ccagtgcgaa cgagtcgtgg
     2821 gcgtacttta tggggcggcg aaaattcgtc gcatcccgtc aggcgagcca gatgtttctg
     2881 tgctggctgg aagaggccat cgttcgccgc gtggtgacgt taccttcaaa agcgcgcttc
     2941 agctttcagg aagcccgcag tgcctggggg aactgcgact ggataggctc cggtcgtatg
     3001 gccatcgatg gtctgaaaga agttcaggaa gcggtgatgc tgatagaagc cggactgagc
     3061 acctacgaga aagagtgcgc gaaacgcggt gacgactatc aggaaatttt tgcccagcag
     3121 gtccgtgaaa cgatggagcg ccgcgcggct ggtcttaaac cgcccgcctg ggcggctgca
     3181 gcatttgaat ccgggctgcg acaatcaaca gaggaggaga agagtgacag cagagctgcg
     3241 taatctcccg catattgcca gcatggcctt taatgagccg ctgatgcttg aacccgccta
     3301 tgcgcgggtt ttcttttgtg cgcttgcagg ccagcttggg atcagccgcc tgacggatgc
     3361 ggtgtccggc gacagcctga ctgcccagga ggcacccgcg acgctggcat tatccggtga
     3421 tgatgacgga ccacgacagg cccgcagtta tcaggtcatg aacggcatcg ccgtgctgcc
     3481 ggtgtccggc acgctggtca gccggacgcg ggcgctgcag ccgtactcgg ggatgaccgg
     3541 ttacaacggc attatcgccc gtctgcaaca ggctgccagc gacccgatgg tggacggcat
     3601 tctgctcgat atggacacac cgggcggaat ggtggcggga gcatttgact gcgctgacat
     3661 catcgcccgt gtgcgtgaca taaagccggt atgggcgctg gccaatgaca tgaactgcag
     3721 cgcaggtcag ctgcttgcca gtgccgcctc ccggcgtctg gtcacgcaga ccgcccggac
     3781 aggctccatc ggcgtcatga tggctcacag taattacggc gctgcgctgg agaaacaggg
     3841 cgtggaaatc acgctgattt acagcggcag ccataaggtg gatggcaacc cctacagcca
     3901 tctaccggat gatgtccggg aaacactgca gtcccggatg gatgcaaccc gccggatgtt
     3961 tgcacagaag gtgtcggcat ataccggcct gtctgtgcag gctgtgctgg ataccgaggc
     4021 tgcagtgtac agcggtcagg aggccattga tgccggactg gctgatgaac ttgttaacag
     4081 taccgatgcg atcaccgtca tgcgtgatgc actggatgca cgtaaatccc gtctctcagg
     4141 agggcgaatg accaaagaga ctcaatcaac aactgtttca gccactgctt cgcaggctga
     4201 cgttactggc gtggtgcaag tgacggaggg cgagaacgcc agcgcggcgc agccggacgt
     4261 aaacgcgcag atcaccgcag cggttgcggc agaaaacagt cgcattatgg ggatcctcaa
     4321 ctgtgaggag gctcacggac gtgaagaaca ggcacgcgtg ctggccgaaa cccccggtat
     4381 gaccgtggaa acggcccgcc gcattctggc agctgcacca cagagtgcac aggcgcgcag
     4441 tgacactgcg ctggatcgtc tgatgcaggg ggcaccggca ccgctggctg caggtaaccc
     4501 ggcatctgat gccgttaacg atttgctgaa cacaccagtg taagggatgt ttatgacgag
     4561 caaagaaacc tttacccatt accagccgct gggcaacagt gacccggcac atacggcaac
     4621 cgcgcccggc ggattgagtg cgaaagcgcc tgcaatgacc ccgctgatgc tggatacctc
     4681 cacccgtaag ctggttgcgt gggatggcac caccgacggt gctgccgttg gcattctggc
     4741 ggttgatgct gaccagacca gcaccacgct gacgtactac aagtccggca cgttccgtta
     4801 tgaggatgtg ctctggccgg aggctgccag cgacgagacg aaaaaacgga ccgcgtttgc
     4861 cggaacggca atcagcatcg tttaacctta cccttcatca ctaaaggccg cctgtgcggc
     4921 tttttttacg ggattttttt atgtcgatgt acacaaccgc ccagctgctg gcggcaaatg
     4981 agaagaaatt taagtttgat ccgctgtttc tgcgtctctt tttccgtgag agctatccct
     5041 tcaccacgga gaaagtctat ctctcacaaa ttccgggact ggtaaacatg gcgctgtacg
     5101 tttcgccgat tgtttccggt gaggttatcc gctcccgtgg cggctccacc tctgaattta
     5161 caccgggata tgtcaaaccc aagcatgagg tgaatccgca gatgaccctg cgtcgcctgc
     5221 cggatgaaga tccacagaat ctggcggacc cggcttaccg ccgccgtcgc atcatcctgc
     5281 agaacatgcg agacgaagag ctggccattg ctcaggtcga agagatgcag gcagtttctg
     5341 ccgtgcttaa gggcaaatac accatgaccg gtgaagcctt cgatccggtt gaagtggata
     5401 tgggccgcag tgtggcgaac aacatcacgc agtccggcgg tacggagtgg agcaagcgtg
     5461 acaagtccac gtatgacccg accgacgata tcgaagccta cgcgctgaac g
//
LOCUS       DAZGAN020000205         5376 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGAN020000205 DAZGAN020000000
VERSION     DAZGAN020000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.206"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            83..1417
                     /gene="garP"
                     /locus_tag="ACHKYO_RS24345"
                     /old_locus_tag="pgaptmp_004869"
     CDS             83..1417
                     /gene="garP"
                     /locus_tag="ACHKYO_RS24345"
                     /old_locus_tag="pgaptmp_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HGU4431554.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1433..2203
                     /gene="garL"
                     /locus_tag="ACHKYO_RS24350"
                     /old_locus_tag="pgaptmp_004870"
     CDS             1433..2203
                     /gene="garL"
                     /locus_tag="ACHKYO_RS24350"
                     /old_locus_tag="pgaptmp_004870"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HGU4431555.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILVP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2233..3123
                     /gene="garR"
                     /locus_tag="ACHKYO_RS24355"
                     /old_locus_tag="pgaptmp_004871"
     CDS             2233..3123
                     /gene="garR"
                     /locus_tag="ACHKYO_RS24355"
                     /old_locus_tag="pgaptmp_004871"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU4431556.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3220..4365
                     /gene="garK"
                     /locus_tag="ACHKYO_RS24360"
                     /old_locus_tag="pgaptmp_004872"
     CDS             3220..4365
                     /gene="garK"
                     /locus_tag="ACHKYO_RS24360"
                     /old_locus_tag="pgaptmp_004872"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HGU4431557.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4398..4774
                     /gene="rnpB"
                     /locus_tag="ACHKYO_RS24365"
                     /old_locus_tag="pgaptmp_004873"
     ncRNA           4398..4774
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKYO_RS24365"
                     /old_locus_tag="pgaptmp_004873"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5169..>5376)
                     /locus_tag="ACHKYO_RS24370"
                     /old_locus_tag="pgaptmp_004874"
     CDS             complement(5169..>5376)
                     /locus_tag="ACHKYO_RS24370"
                     /old_locus_tag="pgaptmp_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431558.1"
                     /translation="QINYTMDLNIEDSSSEELLSDNTLEKDENSPDNGFEVGEYNTYE
                     VYNSENQYFTREDYTYNYDLLNAI"
BASE COUNT         1277 a         1267 c         1509 g         1323 t
ORIGIN      
        1 gccgttacgg cataaatgaa actcataaat aacaccttcc ccataataat aaaaatcagc
       61 ataagtaccc gaggtaaata aaatgattct ggacaccgtt gacgaaaaaa agaaaggcgt
      121 gcatacccgc tatttaatat tactgattat ttttattgtt accgccgtta actacgccga
      181 tcgtgcaacg ctgtctattg ctggtaccga agtggcaaaa gagttgcagt taagtgcggt
      241 ttcgatgggt tacatctttt ctgcttttgg ctgggcctac ttgctgatgc aaatccccgg
      301 cggctggctg cttgataagt ttggctcgaa aaaagtttat acctatagcc tctttttctg
      361 gtcgctattc actttcctgc aaggctttgt tgatatgttc ccgctggcct gggcagggat
      421 ctccatgttc tttatgcgct ttatgctcgg cttctcggaa gcgccatcat tcccggcgaa
      481 cgcccgtatt gtcgccgcct ggttcccgac gaaagaacgt ggtactgcct ccgccatctt
      541 taactcggcg caatatttct cgctggcgct cttttcgcct ctgcttggct ggctgacttt
      601 cgcttggggc tgggagcacg tctttaccgt tatgggggtg attggttttg tgctgacggc
      661 gctgtggatc aaattgattc ataacccgac agatcaccca cgtatgtctg cggaagagct
      721 gaagtttatc tctgaaaatg gcgcggtggt cgatatggac cacaaaaagc cgggcagtgc
      781 ggcagcaagc ggtcccaaac tgcattacat caagcaattg ctctctaacc gcatgatgtt
      841 gggcgtattt ttcggacaat attttatcaa caccatcacc tggttcttcc tcacctggtt
      901 cccgatttat ctggtgcagg aaaaaggcat gtcgattctg aaagtgggtc tggtcgcctc
      961 gattccggca ctgtgtggtt ttgcgggcgg cgtgctggga ggtgtcttct cggattatct
     1021 gatcaaacgc ggtttatccc tgaccctggc acgtaagcta ccgattgtgc tgggaatgtt
     1081 gctggcttcc accatcatct tatgtaacta caccaacaac accacgctgg tggtcatgct
     1141 aatggcgctg gctttttttg gcaaaggatt tggtgctctg ggctggccgg tgatttctga
     1201 caccgcgccg aaagagattg ttggcctctg tggcggcgtc tttaacgtct ttggcaatgt
     1261 tgcctccatt gtcactccac tggtgattgg ctacctggta agtgaactgc actccttcaa
     1321 tgcagcactg gttttcgtgg gatgttcagc gctgatggcg atggtctgct acctcttcgt
     1381 agttggcgac attaaacgta tggaattgca gaaataagca aaggtataag cgatgaataa
     1441 cgatgttttc ccgaataaat tcaaagccgc actggctgcg aaacaggtac aaattggttg
     1501 ctggtcagca ctctctaacc cgattagcac tgaagttctt ggtttggctg ggtttgactg
     1561 gctggtgctg gatggcgaac atgcgccaaa cgatatctcc acgtttattc cgcagttaat
     1621 ggccctgaaa ggcagcgcca gcgcgccagt agtgcgagtg ccgaccaacg agccggtaat
     1681 tattaagcgt cttctggata tcggtttcta taacttcctg attccttttg tagaaacaaa
     1741 agaggaagca gagcaggcgg tggcatcaac ccgttaccca ccggaaggca ttcgcggcgt
     1801 ctccgtttct caccgcgcca atatgtttgg caccgtggcg gattatttcg ctcagtcgaa
     1861 caagaacatc accattctgg tccagataga aagtcagcag ggcgtagata acgtcgatgc
     1921 cattgccgct accgaaggtg tagacggtat cttcgtcggc cccagcgatc tggctgcggc
     1981 attaggccat ctcggcaatg cctcgcaccc ggatgtacaa aaagcaattc agcacatttt
     2041 taaccgtgcc agtgcgcacg gcaaacccag cggtattctc gtgccggtcg aagccgatgc
     2101 gcgtcgttat ctggaatggg gcgcgacgtt tgtggctgtc ggcagcgatc ttggcgtctt
     2161 ccgctctgcc actcagaaac tggctgatac ctttaaaaaa taaccaccac cgcaacaaga
     2221 gagatgattg atatgactat gaaagttggt tttattggcc tggggattat gggtaaacca
     2281 atgagtaaaa accttctgaa agcaggttac tcgctggtgg ttgctgaccg taacccagaa
     2341 gctattgctg acgtgattgc tgcaggtgca gaaacagcgt ctacggctaa agcgatcgct
     2401 gaacagtgcg acgtcatcat aaccatgctg ccaaactccc ctcatgtgaa agaggtggcg
     2461 ctgggtgaga atggcatcat tgaaggcgcg aagccaggta cggtattgat cgatatgagt
     2521 tctatcgcac cgctggcaag ccgtgaaatc agcgaagcgc tgaaagcgaa aggcattgat
     2581 atgctggatg ctccggtgag cggcggtgaa ccgaaagcca tcgacggtac gctgtcagtg
     2641 atggtgggcg gcgacaaggc tattttcgac aaatactatg atttgatgaa agcgatggcg
     2701 ggttccgtgg tgcataccgg ggaaatcggt gcaggtaacg tcaccaaact ggcaaatcag
     2761 gtcattgtgg cgctgaatat tgccgcgatg tcagaagcgc taacgctggc aaccaaagcg
     2821 ggcgttaacc cggacctggt gtatcaggca attcgcggtg gactggcggg cagtactgtg
     2881 ctggatgcca aagcgccgat ggtgatggac cgcaacttca agccgggctt ccgtattgat
     2941 ctgcatatta aggatctggc aaatgcgctg gatacttccc acggcgtcgg cgcgcaactg
     3001 ccgctcacag ctgcggttat ggagatgatg caggcactgc gagcagatgg tttaggaacg
     3061 gcggatcata gcgccctggc gtgctactac gaaaaactgg cgaaagtcga agttactcgt
     3121 taatgacgtc gtgcccggat ggcgtattgc aatccgggcc tggaatccag gccgaataag
     3181 agaaacgccc tccggcgtca tgtggtaaca ggcataggta tgaaaatcgt aatcgcccca
     3241 gactcttata aagaaagttt atctgccagc gaggttgcgc aggcgataga aaaaggattt
     3301 cgggaaattt ttcctgatgc acagtacgtt tctattccgg ttgccgacgg tggcgaagga
     3361 acggtggaag cgatgattgc agccacccag ggttctgaac gtcacgcctg ggttacaggg
     3421 ccgctgggcg agaaagtgaa tgccagttgg gggatctccg gcgatggcaa aaccgcgttt
     3481 attgaaatgg cggcggccag tgggctggag ctggtacctg cggaaaaacg cgatccactc
     3541 gtgaccactt cacgcggcac aggcgagtta atcctgcagg cgctggagag cggcgcgaca
     3601 aacattatta tcggcattgg cggtagcgct acaaatgatg gcggcgcagg catggtacag
     3661 gcgctggggg cgaaattatg cgacgccaac ggcaatgaaa ttggttttgg cggcggtagt
     3721 cttaatactc tgaatgatat tgatatttcc ggcctcgatc cgcgcttaaa agattgcgtc
     3781 attcgcgtcg cttgtgatgt caccaatccg ctggtgggcg ataacggcgc atcgcgcatc
     3841 tttggcccac aaaagggagc cagtgaagcg atgattgttg agctggacaa taacctctct
     3901 cactatgccg aggtcattaa aaaagcgctg catgttgatg tgaaagatgt ccccggcgca
     3961 ggagctgcgg gcggtatggg tgcggcgctg atggcgtttc ttggtgcgga actgaaaagt
     4021 ggtattgaaa tcgtcactac ggcgctgaat ctggaggaac atattcacga ttgtacgctg
     4081 gtgatcaccg gtgaagggcg tattgacagc cagagtattc acggcaaggt accgattggc
     4141 gttgcaaacg tggcgaagaa gtaccataaa ccggtgattg gcattgcggg tagcctgacc
     4201 gatgatgttg gcgttgtaca tcagcatggc atcgatgcgg tcttcagcgt attgaccagc
     4261 ataggtacgt tggacgaagc attccgcggg gcttatgaca atatctgccg tgcttcacgt
     4321 aatatcgccg cgacactggc gattggaatg cgcaacgcgg ggtgacaagg gcgcgcaaac
     4381 cctctatact gcgcgccgaa gctgaccaga cagtcgccgc ttcgtcgtcg tcctcttcgg
     4441 gggagacggg cggaggggag gaaagtccgg gctccatagg gcagggtgcc aggtaacgcc
     4501 tgggggggaa acccacgacc agtgcaacag agagcaaacc gccgatggcc cgcgcaagcg
     4561 ggatcaggta agggtgaaag ggtgcggtaa gagcgcaccg cgcggctggt aacagtccgt
     4621 ggcacggtaa actccacccg gagcaaggcc aaataggggt tcataaggta cggcccgtac
     4681 tgaacccggg taggctgctt gagccagtga gcgattgctg gcctagatga atgactgtcc
     4741 acgacagaac ccggcttatc ggtcagtttc acctgattta acgaaaaccc gccagcgtaa
     4801 accggcgggt ttttgctttt ggaggggcag aaagatgaat gactgtccac gacgctatac
     4861 ccaaaagaaa gcggcttatc ggtcagtttc acctgatgta cgtaataaac cgttccggcg
     4921 ggtttcagat tgttgagtgc gctttattca tgccggatgc ggcgtgaacg ccttatcctg
     4981 cctacaaaag cacgcaaatt caatatattg cagagatggc gtaggcctga taagcgtagc
     5041 gcatcaggca tttttgcggt tgccatcagt ttcaaacccg cgtcagtgag tttttgcttt
     5101 tggtgcacag ggtaagatga ataaatatct acggtattaa taatagactt tgtaagaagt
     5161 tattaagtct atattgcatt taaaaggtcg taattatacg tatagtcctc tctggtaaaa
     5221 tattgattct ctgagttata tacttcatat gtgttatatt ccccgacctc aaaaccattg
     5281 tccggagaat tttcgtcttt ctcgagggtg ttatctgaaa gtaactcttc tgagctagag
     5341 tcttctatgt ttaaatccat agtataatta atttgg
//
LOCUS       DAZGAN020000206         5242 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGAN020000206 DAZGAN020000000
VERSION     DAZGAN020000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.207"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..119)
                     /locus_tag="ACHKYO_RS24375"
                     /old_locus_tag="pgaptmp_004875"
     CDS             complement(<1..119)
                     /locus_tag="ACHKYO_RS24375"
                     /old_locus_tag="pgaptmp_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431559.1"
                     /translation="MIIKNLKHYTPEKPDVPGAMYLKSEDGQDWYECQSLFSA"
     gene            complement(183..1625)
                     /locus_tag="ACHKYO_RS24380"
                     /old_locus_tag="pgaptmp_004876"
     CDS             complement(183..1625)
                     /locus_tag="ACHKYO_RS24380"
                     /old_locus_tag="pgaptmp_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU4431560.1"
                     /translation="MTVRISGVLKDGTGKPVPGCTIELKARRTTETVIVTTVAQGQPG
                     ETGSYSFDVEPGWYRVTLNTEGYAPSYVGDILVKADSEPGTLNKFLMEQDEAQYYPKA
                     LAELEAVAAEILKRAEASAASAEEAKKRAENARGPAGEKGDTGPQGATGAQGPAGATG
                     AVGPKGEPGPKGERGETGPQGPKGDKGDPGGPPGPKGDTGPRGEAGPPGPQGPAGQPG
                     PKGDKGEPGATGPAGPAGPRGETGPAGPAGPAGSVASVPDASTSQKGVVQLSSDTNSD
                     DETKAATPKAVKAVMAEVQAAKTKAEEAATRAAVPGPKGDRGEPGAPGAVGPAGPRGP
                     AGAAGPKGDAGPAGPAGKDGTAGAEGKAGAQGPKGDKGDPGEAGPAGPAGPKGERGEP
                     GPMGPPGKDGATSLVSGLRIGGKKEVAHHEVITFPSLDGAQPTIPGMNIVPDGAVILN
                     IRSQAYKITAVTYGFLQVHNGSQWVTIKTE"
     gene            complement(1810..>5242)
                     /locus_tag="ACHKYO_RS24385"
                     /old_locus_tag="pgaptmp_004877"
     CDS             complement(1810..>5242)
                     /locus_tag="ACHKYO_RS24385"
                     /old_locus_tag="pgaptmp_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU4431561.1"
                     /translation="PIEGPVKGLQSILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTP
                     PEGFESSGAETVLGVEVTKAKPVTRTITSANIDRLRVTFGVQSLVETTSKGDRNPSSV
                     RLLIQLQRNGNWVTEKDVTINGKTTSQFLASVILDNLPPRPFNIRMVRETADSTTDQL
                     QNRTLWSSYTEIIDVKQCYPNTAIVGMQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDP
                     EKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCD
                     QTVPDGFGGTEPRMTFNAYLAQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDV
                     VWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPEAILR
                     YGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICD
                     NDYAGTLTGGRVLSIDAATRTLTLDREVTLPGTGAATVNLINGSGKPVSVDITAHPAP
                     DRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIV
                     DNGARFEPQSGTLNSVIPPAVQHLTVEVSAADSQYLALAKWDTPRVVKGVRFSLRLTS
                     GSGENSRLLTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPAAP
                     ATIELTPGYFQITAVPKLTIYDPTVQFEFWFSEAKIADAAQVETSARYLGTGSQWSVS
                     GPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAAGYLELFREKIGKTHLAEALWA
                     EIDNSQLKDEMAEMQTTITETRNEITQTVSKTLEDQNATIQQIQRVQTDTNNDLNSMW
                     AVKLQQMQDGRRYIAGIGAGIENTPDGMQSQILLAADRVAFINPENGDTTPALVTQGG
                     QTFINEALIKVLTAPTITSGGNPPAFSLTPDGRLTARNADISGNISASSGTLNNVTIA
                     EDCTINGTMSADRIIGDIVKGYTVDMGQGVMTQNQLTFQKMILLKDNMPFDRKLMLMA
                     YSFSRGEFIMKINDKVVVHARSGKVKTGEREVQISKNASRYEPVYSDVGGSASGSWSI
                     PAGQGDVRVEITFRYEADVLFYMGGSILHDKSKIRIHDPVLFYLKSYTDDFDTAS"
BASE COUNT          944 a         1662 c         1349 g         1287 t
ORIGIN      
        1 tccgctgaaa ataacgactg acactcatac cagtcctgac catcctctga tttcaggtac
       61 atcgcacccg gcacatcagg tttttcaggg gtatagtgct tcaggttttt aataatcata
      121 ttattctccg tatttaatgc ccggttgggc ataatggaac gcaacaacca gtgtgagtca
      181 cgttattccg ttttaatcgt tacccactgg cttccattgt gaacctgaag aaagccataa
      241 gttacagcag taattttgta agcctgtgac cggatattca gaattacagc accatcaggg
      301 actatattca tgccgggaat cgttggttgt gctccatcca gtgacgggaa agtaatcacc
      361 tcatggtgtg cgacttcttt cttccctccg atacgcagcc cactaaccag cgaagttgcg
      421 ccatctttcc ccggaggccc catcggtccc ggttcaccgc gctcaccttt gggacctgcc
      481 ggacctgctg gacctgcttc tcccggatcg cctttgtcac cttttggccc ctgtgcgcct
      541 gccttacctt ccgctccggc ggtaccatcc tttcctgcgg ggcctgccgg gcctgcatca
      601 cctttgggac cagctgcacc agccggtccc cgtgggcctg cgggccccac tgcaccaggt
      661 gcgccaggtt cccccctgtc ccccttcggt ccggggactg ctgcccgggt tgctgcctct
      721 tctgccttcg tttttgctgc ctgcacttct gccattaccg ctttcacagc ctttggggtt
      781 gccgcttttg tttcatcatc gctgttggta tcgctgctca gctgtacaac acctttctgt
      841 gatgtgctgg catccggaac actggcgaca ctgcctgcgg gacccgcagg acctgcgggg
      901 ccggtttctc cccgagggcc tgcgggacct gccggacctg ttgcgccggg ttcaccttta
      961 tcccctttcg ggccgggttg ccctgccgga ccctgtggtc cgggtggtcc ggcctctcca
     1021 cgtgggccgg tgtcaccttt cggccccggc ggtccgcccg ggtcaccttt atcgcccttc
     1081 ggtccctgtg gacctgtttc tccccgttct ccctttggcc ccggctcacc ttttggtccg
     1141 accgcccccg ttgccccggc tggtccctgt gcccctgtgg caccctgtgg acctgtgtcc
     1201 cccttctcgc ccgccggtcc ccgggcattc tctgcccgtt tcttcgcttc ctctgcactc
     1261 gccgccgacg cttcagcacg cttcaggatt tccgctgcca ccgcttccag ctctgcaagc
     1321 gctttcgggt aatactgcgc ctcatcctgt tccatcagaa atttattcag cgttcccggc
     1381 tcagaatccg ccttcaccag aatgtcaccc acatacgacg gggcgtaccc ttccgtgttc
     1441 agcgtcaccc ggtaccaccc cggctccaca tcaaaactgt aactgccggt ttcccccggc
     1501 tgcccctgcg ccaccgtggt gactatcact gtctctgtgg tgcgtcgcgc tttcagctct
     1561 atggtgcatc ccggcaccgg cttcccggtg ccgtccttca gcacgcccga tattctgact
     1621 gtcatagatt tctcccataa aaaaaaccgc agtaccggtt acccggtctg cgatcagggg
     1681 tataaattgt gactggctgg tgttaaaggg tgccgtcagt caaaggcacc cgtggataat
     1741 gtacgatgtc cggcttttgt cgttttccgg caatcacgaa cagatggatg aaacctcact
     1801 atccacctgt cacgatgcgg tatcaaaatc gtctgtatag gatttcaggt aaaacagaac
     1861 cgggtcatgg atccgaattt tactcttatc atgaagtatt gaaccaccca tgtaaaaaag
     1921 cacatctgct tcatacctga atgtaatttc aacccgcaca tcaccctgtc cggcaggaat
     1981 actccatgag cctgacgcac ttccgccaac atcagaatat accggctcat atctgctggc
     2041 gtttttggaa atctggacct cccgctcccc ggttttgact ttgcctgacc tggcatgaac
     2101 gacaactttg tcatttatct tcatgatgaa ttcacctctg gaaaatgaat acgccataag
     2161 cataagcttg cgatcaaacg gcatgttgtc tttcagcaaa atcattttct gaaaagtcag
     2221 ctggttctga gtcattacgc cctgccccat atcaaccgtg tatcccttga cgatatcacc
     2281 aatgattcgg tcagcgctca tcgttccatt aatggtgcag tcttccgcaa tcgtcacgtt
     2341 attcagcgta ccggaactgg cactgatatt cccgctgata tcggcattac gggccgtcag
     2401 cctgccgtcc ggcgtcaggg agaatgccgg gggattgccg ccgctggtaa tggtgggggc
     2461 ggtaagaacc tttatcaggg cctcattgat aaacgtctgt cccccctgcg tgaccagtgc
     2521 gggtgtggta tcaccattct ccggattaat gaatgccaca cggtccgctg ccagcagtat
     2581 ctggctctgc atcccgtcag gggtgttctc aataccggca ccgataccgg caatatagcg
     2641 acgaccatcc tgcatctgct gtagcttcac cgcccacatg ctgttcaggt cattatttgt
     2701 gtccgtctgc acacgctgga tttgctgtat ggtggcgttc tggtcctcca gcgttttact
     2761 gaccgtctgc gtgatttcat tgcgggtttc tgtgatggtg gtctgcattt ccgccatctc
     2821 gtccttcagc tgactgttgt caatctctgc ccacagcgcc tctgccagat gcgtttttcc
     2881 tatcttttcc cggaaaagtt ccagatagcc cgcagcatca ttgctcgccc gtccactggc
     2941 ttccacaaaa gcagatttac ccaccaggtt gacgctgcgc acataaaacc agaaatcctt
     3001 tccgggctta atgtgcggac cggagacact ccactgactg ccggtcccca gataacgggc
     3061 agaggtttcc acctgtgcgg cgtctgcaat ttttgcctcc gaaaaccaga actcaaactg
     3121 caccgtcggg tcataaatgg tcagtttcgg gactgccgtt atctgaaaat accccggcgt
     3181 cagctcaatg gtggccggtg ctgcaggcgc attaatccgg aacgtggtgg tggcgggttc
     3241 gccctgctgg ccatagctgt taatcgccct gaccgtcagg gtgtattccc cgagaggcag
     3301 gccactgaaa cggtgctccg tgtctgcggt gatggcggtg gtcagcaggc ggctgttctc
     3361 accgcttcca ctggtcaggc gcagactgaa gcgcacgccc ttcaccaccc gcggcgtgtc
     3421 ccatttcgcc agcgccagat actggctgtc agctgcgctc acctccaccg tcaggtgctg
     3481 tactgccggt gggataacgc tgttcagggt gcctgactgc ggctcaaagc gggctccgtt
     3541 atccacaatg gcttcttttt ccggtacgtg ctgcaccgcc gtgatggcaa aggtgccgtc
     3601 cgtgttttcc cggatggaga cacagcggaa caggcgacgg cgcagtgacg gcagggagag
     3661 tccccacacc ccgtatgtct ccacaccatc aggcagggta ctgacctgta tccggtccgg
     3721 cgcggggtgt gcggtgatgt ccacactcac cggcttaccg ctgccgttaa tcaggttcac
     3781 cgtggcggca cctgtccccg gaagtgtcac ttcacggtcc agcgtcaggg tgcgggtggc
     3841 agcatcaatg gacaggacac gtccgccggt cagggtcccg gcatagtcat tatcacagat
     3901 ttcaataatg tcaccgggtg tgtgccgcag cccctgagac ccgagtgtga aatccaccgt
     3961 ctgcgtttcc agcagttcgg tctttatcac ccacagcccg gcacggtggg cctgaccgcg
     4021 gctggtacag ccgaacgcgt ccatcttcag caggttgcgt ccgtagcgca gtatggcttc
     4081 cgggtcttcc accagttccg tggaggtctg ccagccgttc tgcgggtcgg tgtaattcac
     4141 ctccaccgcc gtgtgccggt ccttcagggc actgaagctg tagcggaacc ccacgccgtt
     4201 atcatccacc accacatcgc tgttggtgta cggccacacc acatccgacg ggcggtcctg
     4261 aacgaacgtc agcgtctggc cgttccatac cggcatacag cgcatcgccg agcagaaatc
     4321 actgagaacg tcccacgcct tacgctgttg tgccaggtac gcattaaagg tcatccgcgg
     4381 ctctgtgccc ccgaatccat ccgggaccgt ctggtcgcag tactgcccga tggcatacag
     4441 cgcccacttg tccacatccg ccgcccccag gcgttttccc atcccgtagc gcgggtgagt
     4501 cagcatgtcc cacaggcacc aggccgggtt gttgctgtat gccggtttca gactgccgtc
     4561 ccagatacca ctgtacgtgc gtttttccgg gtcatagttt gacggcacct ggatgatgcg
     4621 accgcggata tggtagttca ccgtcatctg ctgaccacca aactgctccg catccacctg
     4681 catccccaca atggccgtgt tcgggtagca ctgtttcaca tcgatgattt cggtgtatga
     4741 cgaccacagc gttctgttct gcagctggtc cgtggtgctg tccgccgtct ccctgaccat
     4801 ccggatgtta aaggggcggg gaggcagatt atccagaatc accgaggcca ggaactgtga
     4861 ggtggtcttg ccgttaatgg tgacatcctt ttctgtcacc cagttaccgt tacgctgtaa
     4921 ctgaatcagc agacggacag aggacggatt acggtcaccc tttgacgtgg tctccaccag
     4981 tgactgcacc ccgaaggtca cccgcaggcg gtcaatgttc gctgacgtaa tggtgcgtgt
     5041 caccggtttt gccttcgtca cttccacacc cagtacagtt tcagctccgg aggactcaaa
     5101 gccttccggt ggtgtctgct cctgctcccc ggcgcgccag accgcggtca caccgtgtat
     5161 cacgggatta ccgtccgtgt ccgtcagcgg ggttttgttc accagaatac tctgcaggcc
     5221 tttcaccgga ccttcaatgg gt
//
LOCUS       DAZGAN020000207         5225 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGAN020000207 DAZGAN020000000
VERSION     DAZGAN020000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.208"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(825..1796)
                     /locus_tag="ACHKYO_RS24390"
                     /old_locus_tag="pgaptmp_004878"
     CDS             complement(825..1796)
                     /locus_tag="ACHKYO_RS24390"
                     /old_locus_tag="pgaptmp_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HGU4431562.1"
                     /translation="MKRAPVIPKHTLNTQPVGDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(1796..2962)
                     /gene="sopA"
                     /locus_tag="ACHKYO_RS24395"
                     /old_locus_tag="pgaptmp_004879"
     CDS             complement(1796..2962)
                     /gene="sopA"
                     /locus_tag="ACHKYO_RS24395"
                     /old_locus_tag="pgaptmp_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HGU4431563.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(3550..4305)
                     /gene="repE"
                     /locus_tag="ACHKYO_RS24400"
                     /old_locus_tag="pgaptmp_004880"
     CDS             complement(3550..4305)
                     /gene="repE"
                     /locus_tag="ACHKYO_RS24400"
                     /old_locus_tag="pgaptmp_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HGU4431564.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(5079..>5225)
                     /locus_tag="ACHKYO_RS24405"
                     /old_locus_tag="pgaptmp_004881"
     CDS             complement(5079..>5225)
                     /locus_tag="ACHKYO_RS24405"
                     /old_locus_tag="pgaptmp_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HGU4431565.1"
                     /translation="VLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAML
                     KQLS"
BASE COUNT         1499 a         1281 c         1170 g         1275 t
ORIGIN      
        1 tgcggcctcc atcaggccgt gccgcacctg taaccgggcg accgcatacg aatgcaggtg
       61 cggccccgaa gccgcagcgc gtggaggagg aacgacgaaa cgtgaccggg tgaggaggcc
      121 ggcgacagcg acacacttgc atcggatgca gcccggttaa cgtgccggca cggcctgggt
      181 aaccaggtat tttgtccaca taaccgtgcg caaaatgttg tggataagca ggacacagca
      241 gcaatccaca gcaggcatac aaccgcacac cgaggttact ccgttctaca ggttacgacg
      301 acatgtcaat acttgccctt gacaggcatt gatggaatcg tagtctcacg ctgatagtct
      361 gatcgacaat acaagtggga ccgtggtccc agaccgataa tcagaccgac aacacgagtg
      421 ggatcgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt ggtcccagac
      481 taataatcag accgacgata cgagtgggac cgtggtccca gactaataat cagaccgacg
      541 atacgagtgg gaccatggtc ccagactaat aatcagaccg acgatacgag tgggaccatg
      601 gtcccagact aataatcaga ccgacgatac gagtgggacc gtggtcccag actaataatc
      661 agaccgacga tacgagtggg accgtggtcc cagtctgatt atcagaccga cgatacaagt
      721 ggaacagtgg gcccagagag aatattcagg ccagttatgc tttctggcct gtaacaaagg
      781 acattaagta aagacagata aacgtagact aaaacgtggt cgcatcaggg tgctggcttt
      841 tcaagttcct taagaatggc ctcaattttc tctatacact cagttggaac acgagacctg
      901 tccaggttaa gcaccatttt atcgccctta tacaatactg tcgctccagg agcaaactga
      961 tgtcgtgagc ttaaactagt tcttgatgca gatgacgttt taagcacaga agttaaaaga
     1021 gtgataactt cttcagcttc aaatatcacc ccagcttttt tctgctcatg aaggttagat
     1081 gcctgctgct taagtaattc ctctttatct gtaaaggctt tttgaagtgc atcacctgac
     1141 cgggcagata gttcaccggg gtgagaaaaa agagcaacaa ctgatttagg caatttggct
     1201 gtgttgatac agcgggtaat aatcttacgt gaaatatttt ccgcatcagc cagcgcagaa
     1261 atatttccag caaattcatt ctgcaatcgg cttgcataac gctgaccacg ttcataagca
     1321 cttgttgggc gataatcgtt acccaatctg gataatgcag ccatctgctc atcatccagc
     1381 tcgccaacca gaacacgata atcactttcg gtaagtgcag cagctttacg acggcgactc
     1441 ccatcggcaa tttctatgac accagatact cttcgaccga acgccggtgt ctgttgacca
     1501 gtcagtagaa aagaagggat gagatcatcc agtgcgtcct cagtaagcag ctcctggtca
     1561 cgttcattac ctgaccatac ccgagaggtc ttctcaacac tatcaccccg gagcacttca
     1621 agagtaaact tcacatcccg accacataca ggcaaagtaa tggcattacc gcgagccatt
     1681 actcctacgc gcgcaattaa cgaatccacc atcggggcag ctggtgtcga taacgaagta
     1741 tctccaaccg gttgagtatt gagcgtatgt tttggaataa caggcgcacg cttcattatc
     1801 taatctccca gcgtggttta atcagacgat cgaaaatttc attgcagaca ggttcccaaa
     1861 tagaaagagc atttctccag gcaccagttg aagagcgttg atcaatggcc tgttcaaaaa
     1921 cagttctcat ccggatctga cctttaccaa cttcatccgt ttcacgtaca acatttttta
     1981 gaaccatgct tccccaggca tcccgaattt gctcctccat ccacggggac tgagagccat
     2041 tactattgct gtatttggta agcaaaatac gtacatcagg ctcgaaccct ttaagatcaa
     2101 cgttcttgag cagatcacga agcatatcga aaaactgcag tgcggaggtg tagtcaaaca
     2161 actcagcagg cgtgggaaca atcagcacat cagcagcaca tacgacatta atcgtgccga
     2221 tacccaggtt aggcgcgctg tcaataacta tgacatcata gtcatgagca acagtttcaa
     2281 tggccagtcg gagcatcagg tgtggatcgg tgggcagttt accttcatca aatttgccca
     2341 ttaactcagt ttcaatacgg tgcagagcca gacaggaagg aataatgtca agcccaggcc
     2401 agcaagtggg ctttattgca taagtgacat cgtccttttc cccaagatag aaaggcagga
     2461 gagtgtcttc tgcatgaata tgaagatctg gtacccatcc gtgatacatt gaggctgttc
     2521 cctgggggtc gttaccttcc acgagcaaaa cacgtagccc cttcagagcc agatcctgag
     2581 caagatgaac agaaactgag gttttgtaaa cgccaccttt atgggcagca accccgatca
     2641 ccggtggaaa tacgtcttca gcacgtcgca atcgcgtacc aaacacatca cgcatatgat
     2701 taatttgttc aattgtataa ccaacacgtt gctcaacccg tcctcgaatt tccatatccg
     2761 ggtgcggtag tcgccctgct ttctcggcat ctctgatagc ctgagaagaa accccaacta
     2821 aatccgctgc ttcacctatt ctccagcgcc gggttatttt cctcgcttcc gggctgtcat
     2881 cattaaactg tgcaatggcg atagccttcg tcatttcatg accagcgttt atgcactggt
     2941 taagtgtttc catgagtttc attctgaaca tcctttaatc attgctttgc gtttttttat
     3001 taaatcttgc aatttactgc aaagcaacaa caaaatcgca aagtcatcaa aaaaccgcaa
     3061 agttgtttaa aataagagca acactacaaa aggagataag aagagcacat acctcagtca
     3121 cttattatca ctagcgctcg ccgcagccgt gtaaccgagc atagcgagcg aactggcgag
     3181 gaagcaaaga agaactgttc tgtcagatag ctcttacgct cagcgcaaga agaaatatcc
     3241 accgtgggaa aaactccagg tagaggtaca cacgcggata gccaattcag agtaataaac
     3301 tgtgataatc aaccctcatc aatgatgacg aactaacccc cgatatcagg tcacatgacg
     3361 aagggaaaga gaaggaaatc aactgtgaca aactgccctc aaatttggct tccttaaaaa
     3421 ttacagttca aaaagtatga gaaaatccat gcaggctgaa ggaaacagca aaactgtgac
     3481 aaattaccct cagtaggtca gaacaaatgt gacgaaccac cctcaaatct gtgacagata
     3541 accctcagac tatcctgtcg tcatggaagt gatatcgcgg aaggaaaata cgatatgagt
     3601 cgtctggcgg cctttctttt tctcaatgta tgagaggcgc attggagttc tgctgttgat
     3661 ctcattaaca cagacctgca ggaagcggcg gcggaagtca ggcatacgct ggtaactttg
     3721 aggcagctgg taacgctcta tgatccagtc gattttcaga gagacgatgc ctgagccatc
     3781 cggcttacga tactgacaca gggattcgta taaacgcatg gcatacggat tggtgatttc
     3841 ttttgtttca ctaagccgaa actgcgtaaa ccggttctgt aacccgataa agaagggaat
     3901 gagatatggg ttgatatgta cactgtaaag ccctctggat ggactgtgcg cacgtttgat
     3961 aaaccaggga aaagattcat agcctttttc atcgccggca tcctcttcag ggcgataaaa
     4021 aaccacttcc ttccccgcga aactcttcaa tgcctgccgt atatccttac tggcttccgc
     4081 agaggtcaat ccgaatattt cagcatattt agcaacatgg atctcgcaga taccgtcatg
     4141 ttcctgtagg gtgccatcag attttctgat ctggtcaacg aacagataca gcatacgttt
     4201 ttgatcccgg gagagactat atgccgcctc agtgaggtcg tttgactgga cgattcgcgg
     4261 gctattttta cgtttcttgt gattgataac cgctgtttcc gccatgacag atccatgtga
     4321 agtgtgacaa gtttttagat tgtcacacta aataaaaaag agtcaataag cagggataac
     4381 tttgtgaaaa aacagcttct tctgagggca atctgtcaca gggttaaggg caatttgtca
     4441 cagacaggac tgtcatttga gggtaatttg tcacactgaa agggcaattt gtcacaacac
     4501 cttctctaga accagcatgg ataaaggcct acaaggcgct ctaaaaaaga agatctaaaa
     4561 actataaaaa aaataattat aaaaatatcc ccgtggataa gtggataacc ccaagggaag
     4621 ttttttcagg catcgtgtgt aagcagaata tataagtgct gttccctggt gcttcctcgc
     4681 tcactcgagg gcttcgccct gtcgctcgac tgcggcgagc actactggct gtaaaaggac
     4741 agaccacatc atggttctgt gttcattagg ttgttctgtc cattgctgac ataatccgct
     4801 ccacttcaac gtaacaccgc acgaagattt ctattgttcc tgaaggcata ttcaaatcgt
     4861 tttcgttacc gcttgcaggc atcatgacag aacactactt cctataaacg ctacacaggc
     4921 tcctgagatt aataatgcgg atctctacga taatgggaga ttttcccgac tgtttcgttc
     4981 gcttctcagt ggataacagc cagcttctct gtttaacaga caaaaacagc atatccactc
     5041 agttccacat ttccatataa aggccaaggc atttattctc aggataattg tttcagcatc
     5101 gcaaccgcat cagactccgg catcgcaaac tgcacccggt gccgggcagc cacatccagc
     5161 gcaaaaacct tcgtgtagac ttccgttgaa ctgatggact tatgtcccat caggctttgc
     5221 agaac
//
LOCUS       DAZGAN020000208         5171 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGAN020000208 DAZGAN020000000
VERSION     DAZGAN020000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.209"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1509
                     /locus_tag="ACHKYO_RS24410"
                     /old_locus_tag="pgaptmp_004882"
     CDS             <1..1509
                     /locus_tag="ACHKYO_RS24410"
                     /old_locus_tag="pgaptmp_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431566.1"
                     /translation="WFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFY
                     VGSKQEMALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKI
                     QALSSRPSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLF
                     NQYIQEAREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAP
                     TREDADEEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVL
                     TWCEEHLKPLLEALNSRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLP
                     YAQQEQVMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYCEW
                     MPKLKAEFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDF
                     AVALCMANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            1509..3098
                     /locus_tag="ACHKYO_RS24415"
                     /old_locus_tag="pgaptmp_004883"
     CDS             1509..3098
                     /locus_tag="ACHKYO_RS24415"
                     /old_locus_tag="pgaptmp_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HGU4431567.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVREQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIEAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            3082..4413
                     /locus_tag="ACHKYO_RS24420"
                     /old_locus_tag="pgaptmp_004884"
     CDS             3082..4413
                     /locus_tag="ACHKYO_RS24420"
                     /old_locus_tag="pgaptmp_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HGU4431568.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETNADVHARS
                     FTAAKVARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLARPKAVLWDKRHNNLMYIVENQDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDID"
     gene            complement(4415..4494)
                     /locus_tag="ACHKYO_RS24425"
                     /old_locus_tag="pgaptmp_004885"
     tRNA            complement(4415..4494)
                     /locus_tag="ACHKYO_RS24425"
                     /old_locus_tag="pgaptmp_004885"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            4535..5008
                     /locus_tag="ACHKYO_RS24430"
                     /old_locus_tag="pgaptmp_004886"
     CDS             4535..5008
                     /locus_tag="ACHKYO_RS24430"
                     /old_locus_tag="pgaptmp_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HGU4431569.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLTRSVASSLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
BASE COUNT         1171 a         1365 c         1556 g         1079 t
ORIGIN      
        1 tggtttgagg atgaaagcga ggtctgcatc gcggaaaaat cccgccgcac cgggcttacc
       61 tgggcggaag ccgggcgtaa cgtcattacc gccgcaaagc ctaagcggcg tggtggccgc
      121 aatgtgtttt atgtggggtc aaagcaggag atggcgctgg aatatatttc tgcctgcgca
      181 ctgttctccc gtgcctttaa ccagctggca gatgctgatg tgtatgagca gaccttctgg
      241 gatcgggata aaaaagaaga aattctgacc tacatgatcc gctttccgaa cagtggattc
      301 aaaattcagg cactgtcttc ccgtccgtca aacctgcgcg gccttcaggg ggatgtggtg
      361 attgatgaag ccgcgttcca cgagtcactg gatgagcttc tcaaggcggc aatggcgctt
      421 accatgtggg gcgcgcgcgt gcgtattatc tccacgcata acggcgtcga taatctgttt
      481 aatcagtaca ttcaggaagc ccgcgaaggg cgcaaggatt acagcgtcca ccgcattacc
      541 ctggatgatg ccattgcgga cgggctgtac cgtcgtatct gttatgtcac cggccgcgaa
      601 tggtcaccgg aaagcgagca gaagtggcgt gatgatctct acaaaaacgc cccgacccgt
      661 gaggatgccg acgaggaata cggctgtatc ccgaaaaaat ccggcggtgc ctatattcct
      721 cacgcgctta ttgaaatggc gatgatccgc gacatcccga ttctgacgtt tgaagccccg
      781 gacgacttca tcagccgtgc cgcatggctg cgtgaatcgg aggttttaac ctggtgtgaa
      841 gaacatttaa aaccgctttt agaggcgtta aattcccgtt cccgcttcag cttcggggaa
      901 gactttgccc gcaccggaga cctgtcctgt tttgtgctgc tggaaatcac tgaatccctg
      961 gcaaaacgcg aggtgtttcg cgtggagctg cgcaacctgc cgtatgccca gcaggagcag
     1021 gtgatgatgt acatcctgac ccgcgttccg gcgctggtcg gtgcggcgtt cgacgccacc
     1081 ggtaacggcg gctatctggc cgaagccgcg ctgctggcct ttggcccgga catcatcgac
     1141 tgcgtgatgt tatcgccgaa gtggtactgc gagtggatgc caaaactgaa ggccgagttt
     1201 gaggatcaga atatcctcgt cgcccgccat cagaccacgc ttgatgattt gcgccatgtg
     1261 aaggtggtga acggtatccc gcaaattgac aaggggcgta cgaaggacca gaacgcaacg
     1321 gctgtgaacg cccgccgcca tggcgatttt gccgtggcgc tctgtatggc aaaccgggcg
     1381 tcatacatgg agggcttcat cctggatgaa tcagcctgtc aggcgctgcc ggagcggtcg
     1441 cgggccatgg agggcggtta tcgtgatgac gatgaggcat atcatgaatt tgatcggggg
     1501 tgctggtagt gggacgtata attgatcttg acggaaaacc tttctccttt gacccggaga
     1561 tgcagagtgc cgcgctggat attccgcaga ttgccagccg ttatattgaa catccggcct
     1621 cgggtatcac cccgaaccgg gcggcacagt gccttcgcgg ggctgaacgt ggcgatctga
     1681 ttgcccagtc cgatctggcc gctgacattg aagaaaagga tacccatctt tttgcagagc
     1741 tgggcaagcg acgtcttgcc attcaggggg tgccctggag tattgagccg cccccgaacg
     1801 ccagcgcgaa tgagaaaaag gacgcggaaa tgctcgacga atacctgcat tccgccgact
     1861 ggtttgatgc catgctgttt gatgccaccg atgccatcct gaaaggctat tcctgcatgg
     1921 agattgagca cgggatgctc ggtaaaatgc acatcatccg cgccatccgc tggcgtgaca
     1981 gcgggcattt ctgccttaac ccggatgatt tgagcgaact gcggctgcgt gacggcagcc
     2041 atgccggggt ggcgtttcag ccctttggct ggatagtgca tcagtcacgt tcacgcaccg
     2101 gttacggcgg cgcaacaggg cttgtcagaa cgcttatctg gccgttcatt ttcaaaaact
     2161 attccgtgcg cgatctggct gaatttctgg aggtgtacgg cctgccgatg aaggtcggta
     2221 aatacccgtc cggggcaaca ccggagcaga aaagcgccct gatgcgggcg gtgatggata
     2281 tcgggcgacg tacaggcggg atcatcccgg ccgggatgtc gctggagttt caggcagccg
     2341 cgaacggtca ggccgatccg tttgaaacca tgatttcgtg gggggagcgt tccatctcca
     2401 aagcaatact cggcggcacg ctgaccacgg aagccggaga caaaggcgcg cgctcgctgg
     2461 gtgaagtgca taacgaggtg cgccgggaaa tcagggattc tgatttacgc cagctggccg
     2521 ccacgctgaa ccgcgatctg gtgtatccgc tgtatgccct gaacaccgcc cacgccattg
     2581 atatccgtcg cctgccgcgt atctgtttcc agacaaaaga gccgggggat atcaccaaaa
     2641 tcaccagtgc ggtgatgcag ctcagtacgg ggatggatat tcctgatccc tgggtacggg
     2701 agcagaccgg tattccacag cctgcccccg gtgaagccat cttccgtgtc cgtcagagtg
     2761 gcaatgaacc tgctcagaca gacaaagaga tgccaccgga aaaacaggag aagacagagc
     2821 agacagcgct ggctgccaga ctgccggaag cgaagagcag cccccgagat gaactggacg
     2881 acatggggga tgcggtgcct gcccgccggt tacaggaggc catcgacccg gtactggagc
     2941 cggtcattga agccatcaga acgcgggggc tggcggaggc actggcagac ctgcctgccc
     3001 tttaccgtga aatggatgat tcccgcctga tgacgctgct cagtgatgcc atgtttgctg
     3061 cggaaatgaa ggggatgctg gatggcacag gggattgatt taggttatgc cgccacgctt
     3121 ccctcaaaag aggcggtggc atacttccgc gccaaagggg cgcatatcag ctggaactgg
     3181 ttcgaaacaa acgcagatgt tcatgcccgt tcattcacgg cggcaaaagt ggcacgcctg
     3241 gacgtactga ccacactaca ggcggaagtg cagcgtgctg ttgatgaggg gatttcacag
     3301 aaagcattta tccgcacact gacaccccgc ctgcaaaagc tgggatggtg gggaaaacag
     3361 attgtggtgg acagtgccgg taacgcggaa gaagtgcagc tgggcagtcc ccgccgtctg
     3421 gcgctgattt acaacgtgaa cacccgtgtg gcttacaatg ccgggcgtta cacgcagatg
     3481 atgaacaaca cggacacgca tccgttctgg cagtatgtgg cggtgatgga cagccgcacc
     3541 cgcccggcgc attccgccct taacggtctg gtattccgct atgacgatcc gttctggaat
     3601 acacactacc cgcccaatgg ctggaactgt cgctgccgtg tccggccatt gtctcaggcc
     3661 cgtctggatg cgatggggtt atccgtttca tccggtcagg atcatctctc cacccgcaat
     3721 gttgaggctg gcgtggataa gcagaccggg gaagtcagag aaatgccggt gaccacatat
     3781 tcagatggca ccagaaccat gacaccggat gtgggctggt catataaccc cggttcggcg
     3841 gcgttcggca cagaccaggc gctgatccgt aaactgatcg aggtgaaaag cccggcgctg
     3901 cgggaaatgg tggttcagga gatgaacaac agcccggagc ggcaactggc gttccgcatc
     3961 tgggcaaaaa acatcatgaa aacccggcga ggcggtaacg atatccgcac gctgggcttt
     4021 atgaccgaaa gcattgcgca ggcggtggaa agccggacgg gaacgccacc ggcccgcctg
     4081 ctggcgatga gcggtaaaaa tgtgctccat gcggacagca tgaaacatca gaatgacggt
     4141 atcgccctga cgccggagga tttcgggcgc ttaccggcaa tgctggcaag gccaaaagcg
     4201 gtactgtggg acaaacggca caacaatctg atgtatatcg tggaaaatca ggatggcagt
     4261 gtgcagattg ccgttaatgc accgtacagc ctgaagcgtc agccggataa actggatgtg
     4321 attgttaatg cttaccgggt tatcaatatg gataagctga aatcggatat ccggggcgga
     4381 atgcttgagg tactggaagg agatattgat taacggtcag tggcgggggt cgaacccgca
     4441 tacatgatcc acccggaggc aggcaccgac tttaccagtt aagcgtacac tgaccgcctg
     4501 tttattttat attcatcctt tatgcggagg caatatgtca tccattgatg ccgctgtcgt
     4561 tgttgacgtt acgcgtctcc agcgggtctt tgcccggctt cagtttgtgg gtggtggaaa
     4621 agacctgacc cgcagtgtgg cgtccagcct tctgtcatcg tcagaaatgg cgtttgaaca
     4681 ggaaaaagag ccggacggcg aacgctggca tgactggtca gatccttacc gcaagtggcg
     4741 tacccgtaag ggatacatgc ccggcaaaat cctgacgctg aacggcgatc tggcccgacg
     4801 cctgaccacg gattatggcg atacctgggc gctgattgga tcaaatgagc cttatgcggc
     4861 cattcatcag tgggggggcc tgccaggtat gccgcctgga ccggcggcca ttggtgcacg
     4921 tccgtatatg ggctttgatc aggtggcaga gcaggagatc atggacgaaa tcagaaaacg
     4981 ctttaaaaag gccacagaaa cgccttaatt gtttaaggta tgcaaatgca ttaccttacc
     5041 cccttcaggc gcgtgtcgtg attttctaac ctgaatttaa cgggctttaa aatctgcgtg
     5101 aagcgccttt gtatttttcc cctgaaagca cttcctgaaa tcccgcaaaa cccgccctga
     5161 aaattcccgg c
//
LOCUS       DAZGAN020000209         5049 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGAN020000209 DAZGAN020000000
VERSION     DAZGAN020000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.210"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(183..338)
                     /locus_tag="ACHKYO_RS24435"
                     /old_locus_tag="pgaptmp_004887"
     CDS             complement(183..338)
                     /locus_tag="ACHKYO_RS24435"
                     /old_locus_tag="pgaptmp_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4431570.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYEFEK"
     gene            complement(538..963)
                     /locus_tag="ACHKYO_RS24440"
                     /old_locus_tag="pgaptmp_004888"
     CDS             complement(538..963)
                     /locus_tag="ACHKYO_RS24440"
                     /old_locus_tag="pgaptmp_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4431571.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIIQGIKLEMDGYVERERSGAQASQFSGRTGN"
     gene            complement(1042..1905)
                     /locus_tag="ACHKYO_RS24445"
                     /old_locus_tag="pgaptmp_004889"
     CDS             complement(1042..1905)
                     /locus_tag="ACHKYO_RS24445"
                     /old_locus_tag="pgaptmp_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4431572.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGMD
                     NPAQAIRNARENIKKAASVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            complement(1905..2873)
                     /locus_tag="ACHKYO_RS24450"
                     /old_locus_tag="pgaptmp_004890"
     CDS             complement(1905..2873)
                     /locus_tag="ACHKYO_RS24450"
                     /old_locus_tag="pgaptmp_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HGU4431573.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRVQRRFSALTALQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            complement(2875..4533)
                     /locus_tag="ACHKYO_RS24455"
                     /old_locus_tag="pgaptmp_004891"
     CDS             complement(2875..4533)
                     /locus_tag="ACHKYO_RS24455"
                     /old_locus_tag="pgaptmp_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGU4431574.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLGQ"
     gene            complement(4567..4935)
                     /locus_tag="ACHKYO_RS24460"
                     /old_locus_tag="pgaptmp_004892"
     CDS             complement(4567..4935)
                     /locus_tag="ACHKYO_RS24460"
                     /old_locus_tag="pgaptmp_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431575.1"
                     /translation="MSAELKLVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRKGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
BASE COUNT         1198 a         1402 c         1106 g         1343 t
ORIGIN      
        1 ggcagcataa aatcatcctt ttatgaaaat caacgttttt tgagcactcg cacacaaaaa
       61 attaccgcat gggttgcaac gacaacctgc attttgcgga gcatatgaca aataaagtac
      121 gggtgcgttg aggatgcctg acacatcaga ggtggcggga gattactccc ccgccaggtc
      181 tcttacttct caaattcgta gtctacgaag acagcgacct ccgtctggcc ggttctgatc
      241 cgtacctcac agaggtcttt cctcgttacc agtgccgtca caatgacggt taaacagatg
      301 acgatcaggg cgattaacat cgccttttgc tgcttcatag cctgcttctc cttgccttcc
      361 ggcacgtaag aggctaacct gcatgtgttc agcatgaaat tggcctcaga ttaatgttaa
      421 gcgtcttgca ggacgcgtaa tgttaactgg ggcttttctc cgtctgcctt acggcggcgt
      481 gcccgaggca gacaccctca agcacccgca gtcattctac agtaaaaccc cgcgctttca
      541 atttcccgta cgtccggaaa actgactggc ctgtgcccct gatcgttcac gctcaacgta
      601 tccgtccatt tccagtttta ttccctgaat aatcaacatc ccctcaataa caccttctgc
      661 tttttgcagt acccgcccgg cccagcaatc tgagcgctca tgcttacggc ccagtcccat
      721 aagcgtcatc ccgaacacat aataatcata cagaaaatca tgcaaatcgc tgttgttgac
      781 gttcagttgc gccatgcaat tgctgataat cagcgcatca tcatcggtac actgaggccg
      841 tgatttaacc tttgatggga tcacgccctt aaatccggca gcgacgccgg accatgccac
      901 actctcatga ttatttacca cccatgcacc ccatcgggac agaacctgtt ggatatcacg
      961 cattattctg tactccgctg taaccatact ttttctgatt tgaatgctca gggggaaaat
     1021 gtctactgga tatctccggc ttcattgctc acactcccca accagattga gaatgaccgc
     1081 tgaaccattg tcttccatgt actcatcttt tccgcttgcc agaaaccagc gacacacttc
     1141 aaccgcttca tcacgcgtca caggctttat ttttcccagc aaattttcga gccagaactg
     1201 ccgatcatat actgactcat gatgttcacc tgtgaaaccg tatttgtccc ctgtttctct
     1261 ccccgctgtc tggcgcatct cataaagcca gtcccagtaa ataaactcac gaacaacgtc
     1321 tgacaatgta tgaggctctg gcagtacatc acgatagcca tcaacatatg cacgacgctg
     1381 atcatcaatt tcatttgagc gaacaccgtc aatacttccg gcttttttct ctgccgcagt
     1441 ccagccccag tagtaatcgt cgataaattt cggggaggtg cgaattatcc gctcagcctc
     1501 cacatcgtcg agtgctgcct catagctgcc aaatgtggcc ctgactgatg cggctttttt
     1561 gatattctca cgggcattcc tgatcgcctg cgccgggtta tccatgccta tggttgcgaa
     1621 agcaatctgg aaaggatcaa cattaatctc ctgcaaataa cgtccatacc gttcactcgc
     1681 ttcttccggc gttattttga gtttctccag tgctgcttcg gctgcgtcca gatgtacggc
     1741 ctcgttcagg tgaataacct ccagaatcca gagataagca tcagtctgtt tatgtccggt
     1801 gatcctcctt tgttctggca gaggtttgat atttgccgga acggcgctgt acgctgccgt
     1861 cgggatagtg aataaagctt tatgtacctg attgtccgta cgcatcacgc aaccgccttt
     1921 tttctgtaaa aaaccagttc ccgaacctga tcgccgttca tgagcatatt gttgaaatca
     1981 tcatgatcag gccaccggac acttacccgc tgaatgtcat ttcgggcctg gagatttgca
     2041 tgtgcgcatt cacaggccgc agccattccg gtagcactgt attcatcgcg atcggcaaaa
     2101 ataatcagat gggtgactcc cgccggtacg cggaactttt tcataaaacc actgttaatg
     2161 gttgcccagg tatttacttt gtaaatctga tgtgcggaca acgcggtttc gataccttca
     2221 gctataccca gtgtactggc taccggaaac atcctgatag caactgaacg ggcatggtcc
     2281 agatagttat cgtcctgtaa agatttgagc cttctggcgc tgataccaat gtctgctttt
     2341 ttagctccat caagcaatgt ctgatgcagg taacacagtt ctcccttgtc atcggtcgcc
     2401 agggcataaa gagactggaa catgcgcccg ttataatttt gctggctgtt gaagcgaact
     2461 ccctcaaccg gaagactgaa tatgccacgg gaacgaagat attcagcacc agatgttccc
     2521 tgtagcgccg tgagcgctga aaatcgacgc tgaacgcgcg atctcaggct ttccgacgta
     2581 cagatcacag gcggtttatc tctccggtat tcattccccg tgatgcggtc tatttcagcg
     2641 caaatttcat gaaacgctct tttttgtgtc agagcgatca gtttcatgcc gtcaccgcta
     2701 ccacaaacac agatccacgt tccggtgcca tcacggtcat caatacggaa cttacctctg
     2761 gcaccacaaa ccggacattc ccctttgaag tgctttttac ccgtgactgg cggcagtccg
     2821 taatgtttta aaatttcagg ccagtggcct ttcgctgctt ctgctgtttt catcttactg
     2881 acccagactg tttctgtttt tctgaagttg ttgctttgcc tgcatgatct gccacgactc
     2941 actgccttcc ggcaccccag accggacacc atgaagcagg gacagagaaa gcgcttcggg
     3001 ggatttttcc gtcatcttct ctgcccggct acgccctctg gcaaaccgga tgaatttgtg
     3061 tctgatgtaa ttactgacct ccggggtgat ttccatcgga aaactgctca gcccgtcagg
     3121 ccattcgccg aacttctccc ggaaagtgtg agcgcaccat ccatcgctga ccggtcgccc
     3181 cagagaggca cgctgacgct gatagaattt gatctgactc caccaggcct gtttgtcgct
     3241 tttggtgtaa attttttcgt ccttgctgag tttcctgata ttgcggcggg tatctgtgtt
     3301 cacatcctca ccagccagcg gtttaaatcc gcatttcgga cagacataaa cacctgccgg
     3361 tttcatgaaa tgacaccccg gacattcttt cggcagtttt tcttcccgaa tttcgttacc
     3421 acgtgatgat gacacctgca tcccgtcgtt tttgccgggc agttcgtcat actcaatatt
     3481 ttcaggaaat cccaggcgat gaaccgtacc ggaatggtcg aaaatcaggc atgactcctt
     3541 gcctggcgct gtacgcaatc ctctcccaat cgcctgaagc cagcgaatct ctgattttgt
     3601 cgggcgtgcg taaatgatgc accggacatc actgtcaaat cccgccacca gaacgccaac
     3661 gctgacaatg attttcgtcg ctcccgtttc aaaacgactg atcattaact gacgctcatc
     3721 atgtggcgtt tcagcaacca tcacctctgc gttaattccg gctttgttga actgcatggt
     3781 cacgaagctg gcatgcgttt tattgacgca gaacgccact gtcggtaaat cccttccgtt
     3841 acacagccag ttatcgacaa tatcgccaac cagatccgcg ccgcacataa tctctgccag
     3901 ttgtgattcg tcgtaatccc tgccgtactc cattgagact ttcgttttta caccttccag
     3961 atccggtttc gtgggcgcat aaaattcgta attgctcagt tcgccacgct ggatcagttc
     4021 ccctatagtg gtcggcttga gaagacgctg gtaataacgc cccaggaacg atgaaaaggg
     4081 tgtaccggac aacccaatca ccttcacatt tccctctgca accagtcgtt cgatttccgt
     4141 gaggatgcgg cgcttacgga gatgcgcttc atcaataatc agcaggtcga tattctgagg
     4201 aaaatcacgg cgaatcagcg tatccgcact ggcaatctga atcagtcgtg acgggtcgtt
     4261 attgggatga tcacgccaga taaagctgat ttcatccccg gaaagcccgt actgaacaaa
     4321 gcgctgtgct gtctggttga tcaaaatggt ataaggaacg acaaacagaa cgcgcatgcc
     4381 acggcttacc agaccagcag tgatgaacgc cgccaggcca gttttaccac tccctgtagg
     4441 catgtacatc atgaaggact gatatgtttt ccagtcatga cgcaacatat tcagcgcacg
     4501 ttcctgagca aaattcggct tgatattcag catggtattc tcccgatgac agggctgata
     4561 cagtgattac ttaaaccggg tcgcccggat gacatccaca ccatcggcga tataacgctg
     4621 tggatgtttg ccatcacgga gaaaaacaaa tcccgaaatg ccttcaggaa gcgctcgaat
     4681 cttcatcagt gaacgctgac gaggcgatcg gttatccacc tcaactgcgc aacgctgacc
     4741 atcgggagaa ataaccagaa gatcgataaa tcccttgcga tcacctttca gcctgacagt
     4801 gaaacagcgt tgcaactcac agcccaggga tttgattttc ttttgcagaa cgtagtcgaa
     4861 tgtgaccttg tccattccat gtccgaattc ctcttccaga aggttgatta gtaccagttt
     4921 cagttcagca ctcatatttt aaccatgatg tactagtttg ctgatgcagc cgttttttca
     4981 ggggggcccc cctttagatc gagatctacc taacctctgt acccgtctgt tggaagatcc
     5041 cgttccagt
//
LOCUS       DAZGAN020000210         4987 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGAN020000210 DAZGAN020000000
VERSION     DAZGAN020000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.211"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            266..1429
                     /locus_tag="ACHKYO_RS24465"
                     /old_locus_tag="pgaptmp_004893"
     CDS             266..1429
                     /locus_tag="ACHKYO_RS24465"
                     /old_locus_tag="pgaptmp_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HGU4431576.1"
                     /translation="MEARAEATSARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYD
                     SEDILPGIRGEMNLFASGAIEADVSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALR
                     EKQSIEVLEQSLSRHNAILNDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLK
                     TTLGKLSTWTTNPVTEADLENPFSRMTESRLLTDFTQTPQKGNPSWLASQADVESKKA
                     ALKAQELARYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYGVSEKAAQIAAATGRLD
                     SVARMIDETGRLSLITVRQSRMEMETLRRQEQASARVVDFYRLQFRVARKTLIELLNA
                     ESELYSVGLSRVQTEDQMLHGMLDYLYSQGMLLKWSGVNLSGAEEKVTGQKDR"
     gene            1434..3581
                     /locus_tag="ACHKYO_RS24470"
                     /old_locus_tag="pgaptmp_004894"
     CDS             1434..3581
                     /locus_tag="ACHKYO_RS24470"
                     /old_locus_tag="pgaptmp_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HGU4431577.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRAPEDIPSLAVPVLLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDELMAVYSGYCWFVQPQATQDRRSELPEYDMTGAWFWRVLWRFRPYYGQVILA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDI
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQLNTVLLV
                     ILGTYLIHSDDPHNRITMGALIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNDI
                     VRRPVERESQTHYLTVKDIQGEFRFSDVSFSYKPGLPDALCGLSLCIRPGERVGIIGS
                     IGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGLDMQTGEGGQGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTESVVLKGLSHWSRGRTMVVVTHRLQVLNIVDRIVV
                     MNEGRVMMDGPRDEILKKLSGKQQSGESRAITS"
     gene            3641..4888
                     /locus_tag="ACHKYO_RS24475"
                     /old_locus_tag="pgaptmp_004895"
     CDS             3641..4888
                     /locus_tag="ACHKYO_RS24475"
                     /old_locus_tag="pgaptmp_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HGU4431578.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYARTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
BASE COUNT         1216 a         1098 c         1429 g         1244 t
ORIGIN      
        1 aattgaagta tctcacattt ttattatctt ctgtcagcgc ggaagataat ggcggtagct
       61 tggatcaatt ccggtgtctc tgcatttcaa ttttactcaa tattttaccc ggttgcatca
      121 tgatgcctga ttatatttcc cgcgtgtccc ggaggttcgg gtgaagagaa tattatcttt
      181 ctgttttctt ttctctgtcc acaacgtgtt cgcttctcca ctgccggccc tcctgaggga
      241 tgctctcatt catgatccat cagtgatgga ggcaagggca gaagccacct ctgcccgttc
      301 ccgtgtggag caggctcgtt cagcacactg gccggtcgtc acagcaacag gcagtaaact
      361 cctttcccag agtcaccgct acgactatga ctacgactct gaagatattt tgcccggtat
      421 ccgcggtgaa atgaatctgt ttgcttcggg agctattgag gcggatgtca gccggagtga
      481 gtcggaagcc gagtattacc gttataaagt tgatgaggca ggcgaggaaa caattcgctc
      541 ctttgtttca ttatatcttg atgcgctaag ggaaaagcaa tccattgagg tacttgaaca
      601 gagtctctcc cggcataacg caattcttaa tgatcttaac accatcagta ttcacgacac
      661 cgggcgggag tctgagtttg ttcaggccga agcccgcagg ctgatggtcc ggcagcagat
      721 aaatacccgc acccgagtgc tcaaaaccac gctgggaaaa ctttcgacct ggaccacaaa
      781 tccggtaacc gaagccgatc ttgaaaatcc tttttcccgg atgacagagt ccagattgtt
      841 aactgatttt acgcagacgc ctcagaaagg gaatccgtcg tggcttgcca gtcaggctga
      901 tgtagagagt aaaaaagcgg cgctgaaagc gcaggagctt gcccggtatc cccgggtgga
      961 tttaacggga tcagtgaccc gggatgacag gcagataggt gtcagtctgt cctgggacct
     1021 gttcaaccgt aatgccagtt atggtgtcag tgaaaaggcc gcgcagatag ctgcagctac
     1081 cggacgactg gactctgttg cccgaatgat tgatgaaacc ggacgattat cgctgataac
     1141 ggtcagacaa agtcggatgg aaatggaaac gctgagacgt caggagcagg cctctgccag
     1201 agttgtggac ttttatcgtc ttcagttcag ggtggcaaga aagacactga ttgaattact
     1261 gaatgcagaa agcgaactgt acagtgtcgg gctttcccgg gttcagacgg aggatcagat
     1321 gctccacgga atgctggatt atctgtattc ccagggaatg ctcctgaaat ggagcggagt
     1381 gaatctttct ggtgcagaag aaaaagtaac agggcagaag gacagataaa taagtgaaga
     1441 gtatttttga aggattatca caggtaacgg cattactggg tacacctgta tctgctgaga
     1501 cgctggctgc cggtacagtc cggacagatg tctccgggat tgattttcgt tctgttggcg
     1561 agtttcttcg cagtgaaggg tttgataatc atctttcccg gcgggctccg gaagatattc
     1621 cttcacttgc ggttcctgtg cttcttttac tgaatgcaca ggaagccatt gtggtggtgc
     1681 gtattgaggg tgccggcctg gcccggcagt atcatgtact ccagcccggg ggactccgta
     1741 agacactttc tcacgatgaa ttaatggctg tttattcagg ttactgctgg tttgttcagc
     1801 cacaggcaac acaggatcgc cgttcggaac tgccggagta tgacatgacc ggggcatggt
     1861 tctggcgggt actgtggcgg ttcaggccat attacggaca ggtcattctt gcgagtttta
     1921 tcattaactt tcttgcgctg gtcagttccc tgtatgtcat gaacgtttat gaccgggtca
     1981 tacccaatcg ctcctgggaa acgttatgga tgctgagtat cggggtgatt gcagccattc
     2041 tgtttgagtg tgctgcaaag ttaatccgga gctatctgac ggatattgcg ggtaagaagg
     2101 cagacctgat tatcagctca gcactgttaa ggcgggtgat ggcagtgaaa atgtcggaca
     2161 ggccggcgtc ttccggttca tacgccagca atttgcggga atatgagtct gtcagggagt
     2221 ttatgaccag cgccacgctg ctggcttttg tggatttgcc gtttatcctg cttttcatcg
     2281 gtgtcatcgg gcttgtgggg ggctggcttg ctgttgttcc tcttacactg gttccgctgg
     2341 tgattctggc aggaatatta ctgcagcgac cgctttcgcg tcatattaat gcctccatgc
     2401 gggaatcatc gcagcgccag ggattacttg tggaggctct ggacggaata gaaaccctga
     2461 agctgaataa tgcagttaac tgggtccagc gccgctggga tggatataca gccagtgtcg
     2521 cccgctcgtc catacgggta aaaaatacca gtaatgtcat aatctgcttt tccacagcag
     2581 ttcagcaact gaatactgtt ctgctggtca ttctggggac gtaccttatt cacagtgatg
     2641 acccacataa ccgaatcacc atgggggcac tgattgccgc agttattctt tccggtcgtg
     2701 cgctggcacc gcttggtcag atagcatcac tggccacccg ttttcagcag gcccgcctgg
     2761 ccatgcaggg ggtgaatgac attgtccgcc gtccggtgga gcgggaaagt cagacacatt
     2821 atctgacagt gaaggatatc cagggagagt ttcgtttcag tgatgtctcc ttttcttata
     2881 agcccggatt acccgacgca ttgtgcggtc tgtcgctctg tatccggccc ggagagcggg
     2941 tgggtattat tggctcaata ggttgtggta aatcgacgct gctgaaaatg atggtggggc
     3001 tgtaccgtca gaacagggga caaattactc ttgacggggt tgcgatgcag caactggacc
     3061 cctgttttgt ccgggataaa gtgtttcttc tggaacagaa tccccggctt tttctgggaa
     3121 ctctgcgtga aaatctgcag ctttcgcgtc tgccgggaag cctgtcggat gtggatatga
     3181 ttaatgcagc cggacgtttc gggtttgacc gttttatccg gcatcatccc cgcggacttg
     3241 atatgcagac aggggaaggc ggtcagggac tgtcaggagg acagaggcag atggttgccc
     3301 tgacgagaat ggtactgggt tctccccggg tggtactgct tgatgagccc acgacaggtc
     3361 tggacctgca gactgaatct gttgttctga aaggtctgag ccactggagc cggggacgca
     3421 caatggtggt ggtgacacat cgtctgcagg ttctgaatat tgttgaccgt attgtcgtga
     3481 tgaatgaagg gcgggtaatg atggacggtc cccgtgatga aatactgaaa aaactgtcag
     3541 ggaaacagca gagtggggag agcagagcga taacttcatg agcgtcaacc ggataagaaa
     3601 aaagagcaga tatgtttaac agtttgtgga ccgcagggat atgacagaaa aaacggaacg
     3661 gaataaagcg tcgtcagagg tggtcaggca gtcagacctg gcgctgttga cagatgtaca
     3721 ggcagccctt cagcaggaaa agcatcatgc ccttttcctg atggtgattt ttctgctggt
     3781 actggtagtg acgtttgtta tctgggcatg gaacagtcct ctggatgaag tgacccgagg
     3841 tcaggggagt attattcccg gcagcaggga gcaggttata cagacccttg acccgggtat
     3901 actgaaatca cttgaggtca gggaaggcga tattgttgag aaaggtcagg tactgctgac
     3961 actggatgat acccggagct ctgccatgct gagggaaagt gaagcccggg tgaataacct
     4021 ggaggccgtc agggcaagac tccgtgcaga agcttattcc gaatcactga catttccgga
     4081 taacgttcct gaggatttgc gggagcggga aagtacggtc tattttctcc ggaagacgga
     4141 gctggcgcag agcattgccg gtctgaagca gagtaaggcg ctgctggaca gggaaatagc
     4201 aatgacgcgg ccaattgtgc gtgaaggggc aatgtcggag gtcgaactgt tacgtatgca
     4261 gcgtcagtca gcggagctgc agttacagat ggatgagaaa cagaataaat acctgacgga
     4321 agccggtgct gaactggtga agacagaagc agagcttgct caggcaaaag agaatatggc
     4381 tggcagagcc gacccggttg agcgttcacg tctcagggcc cccctgagag gtatcgtaaa
     4441 aaatatacgg gtaaacacac tgggaggggt ggtttctgca ggacaggaca taatggaaat
     4501 tattccgctg gaagaccagt tgctgattga agcttatata aatccacggg atgttgccta
     4561 tgccagaaca gggatgccgg cacttgtaaa actgacggct tatgactatg ccatttatgg
     4621 tggtcttgac ggcgtggtga cactggtaag tccggatact ttacgtgacc agaaacggcc
     4681 gggagattta aaactggacc cgaatgaagc ttattaccgg gtactggtta ccaccagtaa
     4741 taattatctg acagacagga acggaaaaag attgccagtt attcctggca tgatagccag
     4801 cgtggatata aaaaccgggc gtaaaacggt atttcagtat ctgattaaac cgataaccag
     4861 aatgaagcag gcacttcagg aaagataata aaaaggtctt ccggggaaag tattttaaat
     4921 caattttaaa cgggcagaat gatttcactt tattctgccc gggtcattgc gcatgcctgt
     4981 atgtgat
//
LOCUS       DAZGAN020000211         4902 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGAN020000211 DAZGAN020000000
VERSION     DAZGAN020000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.212"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1480
                     /locus_tag="ACHKYO_RS24480"
                     /old_locus_tag="pgaptmp_004896"
     CDS             <1..1480
                     /locus_tag="ACHKYO_RS24480"
                     /old_locus_tag="pgaptmp_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4431579.1"
                     /translation="NVMLGGTLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNY
                     TGNNGLMTFNATLGGDNSPTDKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGG
                     NSAGNFALTTGTVEAGAYVYTLAKGKGNDEKNWYLTSKWDGVTPPDTPDPINNPPVVD
                     PEGPSVYRPEAGSYISNIAAANSLFSHRLHDRLGEPQYIDSLHSQGSASSMWMRHVGG
                     HERSRAGDGQLNTQANRYVLQLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVG
                     YKSDGRISGYSAGLYATWYQNDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGV
                     TASVEGGYTFEAGTFSGSEGTLNTWYVQPQAQITWMGVKDSDHTRKDGTRIETEGDGN
                     VQTRLGVKTYLNSHHQRDDGKQREFQPYIEANWINNSKVYAVKMNGQTVGREGARNLG
                     EVRTGVEAKVNNNLSLWGNVGVQLGDKGYSDTQGMLGVKYSW"
     gene            1689..1955
                     /gene="hipB"
                     /locus_tag="ACHKYO_RS24485"
                     /old_locus_tag="pgaptmp_004897"
     CDS             1689..1955
                     /gene="hipB"
                     /locus_tag="ACHKYO_RS24485"
                     /old_locus_tag="pgaptmp_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HGU4431580.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            1955..3277
                     /gene="hipA"
                     /locus_tag="ACHKYO_RS24490"
                     /old_locus_tag="pgaptmp_004898"
     CDS             1955..3277
                     /gene="hipA"
                     /locus_tag="ACHKYO_RS24490"
                     /old_locus_tag="pgaptmp_004898"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HGU4431581.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            3584..3792
                     /locus_tag="ACHKYO_RS24495"
                     /old_locus_tag="pgaptmp_004899"
                     /pseudo
     CDS             3584..3792
                     /locus_tag="ACHKYO_RS24495"
                     /old_locus_tag="pgaptmp_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            4225..4788
                     /gene="fimA"
                     /locus_tag="ACHKYO_RS24500"
                     /old_locus_tag="pgaptmp_004900"
     CDS             4225..4788
                     /gene="fimA"
                     /locus_tag="ACHKYO_RS24500"
                     /old_locus_tag="pgaptmp_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4431582.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIEILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT         1348 a         1057 c         1260 g         1237 t
ORIGIN      
        1 gaacgttatg cttgggggga cactgcgtgt cgctaaaacc acaatcggcg gcaacctgga
       61 gaatggcggc acggttcaaa tgaatagcga aggggggaaa ccggggaatg tactgaccgt
      121 taacggcaac tataccggaa acaatggcct gatgacgttc aacgcgacgc tgggcggcga
      181 taattcgccc accgataaaa tgaacgtgaa aggcgatacc caagggaaca ctcgcgttcg
      241 ggttgataac attggcggcg tcggtgcaca aacggtcaac ggtattgaac tcattgaggt
      301 tggcggtaat tctgcaggta acttcgcgct gaccaccgga actgtcgaag ctggggctta
      361 cgtctacacg ctggctaaag ggaaggggaa tgacgagaaa aactggtatc tgaccagtaa
      421 atgggacggc gtaacgccac cggatacgcc cgaccccatc aataatcccc ctgttgtgga
      481 tccggaaggc ccatcagttt atcgcccgga ggccggaagc tatatcagca acattgccgc
      541 agccaactcg ctgtttagcc atcgtttaca cgaccgtctg ggtgagccgc agtatataga
      601 ttcactgcat tctcaggggt cggcaagcag tatgtggatg cgtcatgtcg gagggcacga
      661 acgttcaagg gccggtgacg gtcagctaaa tactcaggct aaccgctatg tattgcagct
      721 aggcggcgat ttggcgcagt ggagtagcaa cgcgcaggat cgctggcatc ttggcgtgat
      781 ggcaggttac gctaatcagc acagtaatac tcagagtaat cgtgtgggtt ataaatcgga
      841 tgggcgcatc agcggttaca gcgctgggct gtacgcgacc tggtatcaga acgatgcgaa
      901 taagaccggc gcttatgttg acagctgggc gctgtataac tggtttgata acagcgtcag
      961 ttccgataac cgttctgctg acgactatga ttctcgcggt gtgacggcct ctgttgaggg
     1021 tgggtatacc tttgaagcgg gaacatttag cggcagcgaa gggacgctga atacctggta
     1081 cgtccagcca caggcgcaaa tcacctggat gggtgtgaaa gattccgacc atacccggaa
     1141 agacggaacg cgcattgaaa cggaaggcga cggaaatgtg caaacgcgac ttggggtgaa
     1201 aacctacctg aacagccatc accagcgtga cgatggtaaa cagcgtgagt tccagcctta
     1261 cattgaagcg aattggatca acaacagcaa agtctacgct gtgaagatga atggtcaaac
     1321 cgtaggccgt gaaggtgcgc gtaatctcgg tgaagtacgt accggggttg aggcgaaagt
     1381 aaataacaac cttagcctgt gggggaatgt cggtgtgcaa ctaggtgata aaggctatag
     1441 cgatactcag ggcatgctgg gagtgaaata tagctggtaa accgtataag ccgcatgtcg
     1501 agatggcatg cggcttaata ttgccgactt caaaggcttt aaagcctccc ttttatccgc
     1561 gatcgcggat atcgcagcgt ttatcctgta cagcggataa gatgtgtttc cagattgact
     1621 ttatcctcac taaaggataa aactgataat atccccttaa gcggataaat ttgctgtgga
     1681 cgtatgacat gatgagcttt cagaagatct atagcccaac gcaattagcg aatgcaatga
     1741 aactggttcg ccagcaaaat ggctggacgc agagcgagct ggcgaaaaaa attggcatta
     1801 agcaggcgac aatttccaat ttcgaaaaca accctgacaa taccacgctc acgacatttt
     1861 ttaagatttt acagtcgctt gaactctcaa tgacactatg cgacacgaaa aatgcctcgc
     1921 cagaatcaac agaacagcaa gatctggagt ggtaatgcct aaacttgtca cttggatgaa
     1981 caaccagcgg gtaggcgagt taacgaagtt agccaacggc gcgcacacct ttaagtatgc
     2041 accggagtgg ttagccagcc gttatgccag accgttgtca ctttcgctgc cattgcagag
     2101 ggggaatatc acctcagatg ccgtatttaa cttcttcgat aacctgttac ccgatagccc
     2161 gattgtacgt gaccggatcg ttaaacgtta tcatgctaaa tccagacaac cgtttgattt
     2221 attgtcagaa atagggcgag acagcgttgg tgccgtaacg ttattacccg aaaatgaaac
     2281 cataacgcgc ccgataatgg catgggaaaa gcttactgaa gccaggcttg aagaagtatt
     2341 aacggcttat aaagcagata tccctctggg catgattaga gaagaaaatg actttcgcat
     2401 ctcggttgct ggcgcgcagg agaagacagc gctgcttaga ataggcaatg actggtgcat
     2461 tccgaaagga ataacgccga cgacgcacat cattaaatta ccgattggcg aaatcaggca
     2521 gcccaatgcg acgctcgatc tcagccaaag cgttgataat gagtattact gtctgctgct
     2581 ggcgaaagaa cttgggttga atgttccgga cgcagaaatc attaaagcgg gaaatgtgcg
     2641 cgcgttagcg gtcgaacgtt ttgacaggcg ttggaatgct gagcgaacgg ttttacttcg
     2701 cttgccacag gaggatatgt gtcagacatt cggtttacct tcatcggtga aatatgaatc
     2761 agatggaggc ccaggcatcg cgcggatcat ggcttttttg atggggtcca gcgaggcgct
     2821 gaaagatcgc tatgatttta tgaaattcca ggtcttccag tggttgattg gcgcaacgga
     2881 cggtcatgca aaaaacttct ccgtatttat tcaggctggc ggcagttatc gactcacgcc
     2941 attttacgac atcatttcag catttccggt ccttggcggt acgggaatac acatcagcga
     3001 tctcaaactg gcaatggggc ttaacgcatc caaaggcaaa aaaacggcaa tcgataaaat
     3061 ttatccgcga cattttttgg cgacagcaaa ggtgctgaga ttcccggaag tgcagatgca
     3121 tgaaatccta agtgactttg ccagaatgat tccggcagca ctggataacg tgaagacttc
     3181 attaccgaca gattttccgg agaacgtggt gacggcagtt gaaaccaatg tgttgaggtt
     3241 gcatggacgg ttaagccgag aatacggtag taagtgagat atggggtttg gtcgttgtta
     3301 atgaacaatc atgaccgcca acgactggat tcttccttgg tttatactgg tgcgcataat
     3361 gtatattatg ttaaatcatg tctgagtgat gcgaagtgct tttctggtag tcgttattcg
     3421 ttcagggttc aagaacttaa gcctctcctc tttcataatt ggcctgtgca tggagaaata
     3481 gcctggttat tccctgcccc atgctaagag cgcagtgtct gtcgcataga cactgcatac
     3541 gaaaacacca gagggtgctg ttacttattt aaggacggat caagcgggaa tgagtccact
     3601 ccggtatcag aaatggctac gtctcaatga agtcaggcga ccgatgctga atgaacatta
     3661 cgatgtcacc acagcagcct atgctgtcgg ttacgaaagc tatcccattt cagtcgggaa
     3721 tattcgcgga tgtttggaga gtcacccaag agagatatta ccaggttaag aaagtctgtc
     3781 ggtcagcttt aataaaccct gcacttatct gtttagagca attcggtgtt agtttcagca
     3841 agcaaacatt aaccatagct aatgatttat agccatatta accattggta gcccttgacc
     3901 tttcctgaca cttcccttgt tcgcaataat attctgttta gtcacggttt ataaataaca
     3961 tgcataatga aatcgtcatg ttatttagcg acataaaatg tttttgcaaa tactctcttc
     4021 aacaagtgca gcaataaact tgttgtgatt aggtgtcctt gtgatttaaa gatattatcg
     4081 ttcttcgtga aatgtaataa ccctggaaac tatattctca gggcaacaac caatcaagaa
     4141 tgattcttgt ttttaagcaa taacagaatg acttaattgt taaccctctt tattcagcta
     4201 cttaaatata aattttggag aattatgaaa ctcaaacatg ttggtatgat tgtcgtttct
     4261 gtgttggcga tgtcgtctgc tgcggtaagc gcagccgagg gtgatgaatc agtaacgacc
     4321 actgttaatg gcggtgttat tcattttaaa ggtgaagtgg taaatgccgc ttgtgcaatt
     4381 gattcagaat caatgaacca aacggttgag ctgggtcagg ttcgttcttc tcgcctggct
     4441 aaagcgggtg acctcagctc cgccgttggc ttcaatatca agctgaatga ttgtgatacc
     4501 aatgtttcca gtaatgcagc tgttgcattc ctgggtacta ctgtcaccag taatgacgat
     4561 acgttagcgc tgcagagttc agcggcaggc tctgcccaaa atgtcggtat tgaaattttg
     4621 gaccgtacgg gtgaggtatt aatacttgat ggggccactt ttagtgctaa aaccgacttg
     4681 attgatggca cgaatatact gccattccag gctcgttata ttgctctcgg gcagtccgta
     4741 gctggtactg caaacgctga tgcgaccttc aaagttcaat atctataatt caatcttaag
     4801 gtggctatac gtcatctgaa attgaaacag ggtgtgtgtg gcatggctac gcacacccga
     4861 gcaatttacc aatattcatc tcatttttaa tgagaaatgg tt
//
LOCUS       DAZGAN020000212         4893 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGAN020000212 DAZGAN020000000
VERSION     DAZGAN020000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.213"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            155..1129
                     /gene="hemB"
                     /locus_tag="ACHKYO_RS24505"
                     /old_locus_tag="pgaptmp_004901"
     CDS             155..1129
                     /gene="hemB"
                     /locus_tag="ACHKYO_RS24505"
                     /old_locus_tag="pgaptmp_004901"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HGU4431583.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1235..2086)
                     /gene="tauD"
                     /locus_tag="ACHKYO_RS24510"
                     /old_locus_tag="pgaptmp_004902"
     CDS             complement(1235..2086)
                     /gene="tauD"
                     /locus_tag="ACHKYO_RS24510"
                     /old_locus_tag="pgaptmp_004902"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HGU4431584.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2083..2910)
                     /gene="tauC"
                     /locus_tag="ACHKYO_RS24515"
                     /old_locus_tag="pgaptmp_004903"
     CDS             complement(2083..2910)
                     /gene="tauC"
                     /locus_tag="ACHKYO_RS24515"
                     /old_locus_tag="pgaptmp_004903"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HGU4431585.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPLQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2907..3674)
                     /gene="tauB"
                     /locus_tag="ACHKYO_RS24520"
                     /old_locus_tag="pgaptmp_004904"
                     /pseudo
     CDS             complement(2907..3674)
                     /gene="tauB"
                     /locus_tag="ACHKYO_RS24520"
                     /old_locus_tag="pgaptmp_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
     gene            complement(3687..4649)
                     /gene="tauA"
                     /locus_tag="ACHKYO_RS24525"
                     /old_locus_tag="pgaptmp_004905"
     CDS             complement(3687..4649)
                     /gene="tauA"
                     /locus_tag="ACHKYO_RS24525"
                     /old_locus_tag="pgaptmp_004905"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4431586.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1138 a         1406 c         1236 g         1113 t
ORIGIN      
        1 tcagtgaata tgaaattatt gctatttcta ttacaaagat gtcctggtct ctctttcatt
       61 ctcttcttgt caacatcacg acaactttcg taaaacactc ctgccccgct tcaggtatac
      121 tgtgcacctt aattccacaa acatcaggca gaccatgaca gacttaatcc aacgccctcg
      181 tcgcctgcgc aaatctcctg cgctgcgcgc tatgtttgaa gagacaacac ttagccttaa
      241 cgacctggtg ttgccgatct ttgttgaaga agaaattgac gactacaaag ccgttgaagc
      301 catgccaggc gtgatgcgca ttccagagaa acatctggca cgcgaaattg aacgcatcgc
      361 caacgccggt atccgttccg taatgacttt tggcatctct caccataccg atgaaaccgg
      421 cagcgacgcc tggcgggaag atggactggt ggcgcgtatg tcgcgcatct gcaagcagac
      481 tgtgccagaa atgatcgtca tgtcagacac ttgtttctgt gaatacactt ctcacggtca
      541 ctgcggtgtg ctgtgcgagc atggcgtcga caacgacgcg actctggaaa atttaggcaa
      601 gcaagccgtg gttgcagctg ctgcaggcgc agacttcatc gccccttccg ccgcgatgga
      661 cggccaggta caggcgattc gtcaggcgct ggacgctgca ggctttaaag atacggcgat
      721 tatgtcgtat tcgaccaagt tcgcctcttc cttttatggt ccgttccgtg aagctgccgg
      781 aagcgcatta aaaggcgacc gcaaaagcta tcagatgaac ccaatgaacc gtcgcgaggc
      841 gattcgtgaa tcactgctgg atgaagccca gggcgcagac tgcctgatgg ttaaacctgc
      901 tggagcatac ctcgacatcg tgcgtgagct gcgtgaacgt actgaattgc cgattggcgc
      961 gtatcaggtg agcggtgagt acgcgatgat taagttcgcc gcgctggcgg gtgctataga
     1021 tgaagagaaa gtcgtgctcg aaagcttagg ttcaattaag cgtgcgggtg cggatctgat
     1081 tttcagctac tttgcgctgg atttggctga gaagaagatt ctgcgttaat ttttttcctc
     1141 tcagaaaggg ggagttaacc tgttgaaagt acggtgaacg ccccctcacc ctaaccctct
     1201 ccccaagggg gcgagggaac cgtccgcact cgtatcaccc cgcccgataa aacggtttat
     1261 ccccaagaat cgtcgcccga tgcataatcc gccgctgtgg caggtaatcg gcattggcat
     1321 agtgctgagt cacgcggtta tcccaaatcg caatatcatt cggttgccag cgccaacgca
     1381 cctgaaactc cggtttagtg atatgggcga ataaaaagcc taacaaggct tcgctctctt
     1441 tctcgctgac atcgacaatt cgcgtagtaa agccttcatt cacaaacagc gcctgtttac
     1501 cgctcaccgg atgcgttcgc accaccggat gtagcaacgg aggatttttc gcgaccgcct
     1561 cgcgccagcg ttgatgttcc tcctcggttt tgcggtattt gtattccggg aacgatttac
     1621 ggaaatcatg ctccgcacgc agcccgctca gcagctggcg gaagggaaca gagagcgcct
     1681 cataggccgc aataccgctg gtccagagcg tatcaccgcc ggtcgaaggt aattctttag
     1741 ctgccagaat cgcccctgcg ggtggcgttt caataaatgt cacatcggta tgccagttgt
     1801 cgttatccgg cggattatcg ttatgggtat ccagcacgat gatctcgtca accccttcgg
     1861 catgagggta aacagggtga atatgcagtt cgccaaaacg ctgggccagc gcgcgttgct
     1921 gctgcggcgt aatagtttga tcgcgcagaa acaccacctg atggcgcagc accgcatggt
     1981 aaagctgttc aaactgatta tcgcttaacg ggcgcgtcag gtcggcaccc gaaatttgtg
     2041 cgccgatata cggccccagc ggggtaatgc tcagacgttc actcattgta cttctccatg
     2101 ccagggcgtc agacggcgct gtaacgcgcg caaacccagt tctaaaagaa aggcgataat
     2161 cgcaatcacc gcgatccccg ccagcaccac gtcagtcgcg agaaattcac ccgctgactg
     2221 aaccataaat cctaaaccgc gcgtcgcggc aatcagctcc gccgctacca gcgtagacca
     2281 gcccacaccc agaccaatgc gtaatccggt gaggatttcc ggcagcgcgc cgggcaaaat
     2341 gacaaaccac agcacctgcg cacggctggc acccagcgac tgggcggcac gaatgcgaac
     2401 ctgctgcgcg cttttcaccc ccgccagcgc cgacatcgcc accggtgcaa aaatcgctaa
     2461 atagatcagt aagatcttcg aggtttcacc aataccaaac cagatcacca tcagcggcaa
     2521 ataagccagc ggcggcaccg gacgataaag ttcgattacc ggatccagaa tgccgcgcac
     2581 cgtcgggctt agccccatgg cgatcccgac cggaataccg atcaccaccg ccgcgagtag
     2641 cgccagcaca atgcgcgtca ggctggctgc cagatgctgc cacagcgtgg cgtccataaa
     2701 tccctgcggt ccggcaatgg tgagcagttt tgccagtact tgttgcagcg gcggcaaaaa
     2761 cagcgggcta atcagttgca gcgccgccac cgtccaccat acagtgagta aaaccgctaa
     2821 cgtgccaatg cttaaggtca cctgacgcga gagcggccag cgccatttca gccgccgcga
     2881 atgcagtttt tcattaatga gcacactcat gagaacgcct cccgttgctc aaatacgcgg
     2941 cttaaaacat attcgcgcat ggcgatgaat tatggatcgg acttgatgct gcggctcgac
     3001 tctcccgcaa caaagcggcg agcaaagttg agcggcagcc gctccagcac acggccaggg
     3061 ccggatgaaa gcagaaccag ttcagtcgcc ataaacaccg cttcttctat atcgtgggta
     3121 atcaacagca cctgcttgcc cgtctcctgc cagagtttca gcagcagagt ttgcatctgg
     3181 tcgcgggtga aggcgtccag cgcaccaaac ggttcgtcga gtaataacag ctggggattc
     3241 gccgccagcg cgcgggcaat ccccacccgc tgacgttgac caccggaaag ctgccagata
     3301 tagcgttttt ctgcgccttc cagccccact tttttcagca tctggtgcgc gatttccagt
     3361 cgctgcattt tctctatacc tgccaattgc aggccgaacg ccacgttgtc ctgtacattg
     3421 cgccacggta gtagcccttc attctgaaaa accaccccgc gctctgctcc cggtccctca
     3481 atacgcttac ccgccagttg aatgctgcca tgctgataag gcacaaaacc ggcaatcaga
     3541 ttcagcaacg tggttttacc gcagccggac ggccccagca ccaccagtag ctcgccgctt
     3601 tccagcgtca ggttgatatc ttccagtgcc ggtttgccgc cataatcggc atagagatga
     3661 gagatttgca gcatctgcgc ctccttttat tgcacgaagc gcgaggtaac gtactggctg
     3721 taatcattcg ctacagccgg gaccttgccc tgctctttca aaaactgcgc ggtgtcgatg
     3781 attgctttgt tcaccggtcc ggtcagttct gccgtttgtt gctgcggcgt cagataggta
     3841 tttcccttca ccagccccgg aacgtcacct tcaggcacgc cgcttaaacg cgccagtttg
     3901 ctgatgtttt ccggctgttt cagccacgcg tctgggttag caatgtacgg ttgctgagca
     3961 tcgatggcgc ttttagcgaa cgctttcacg acctcaggat gtttctcggc aaaatctttg
     4021 cgcaccaccc agacgtccag cgttggcgca ccccactgcc cgacctgttc agaatcggtc
     4081 aacaccttgc cgtctttttc cagggcgtta accgccggtg cccagacata agcaccatca
     4141 atatctcccc gctgccaggc agcgataatc gcgggcggct gcaggttcac aatctccact
     4201 tgcccgggtt taatgcccca gtgtttcagc gccgccagca ggctgtagtg ggtggtagag
     4261 ataaacggta cggctatgcg tttgccaatc agatcttccg gtttgctgat agttttcttt
     4321 accaccagcg cttcggagtt acccagtttt gacgccagca agaaaacttc aatcggcacc
     4381 tgttggctgg ctgcaaccgc taacgggctg gaaccgaggt tgccgatttg cacatcgcca
     4441 gaagccagcg cccgcacgat gctggctccg ctgtcaaact tacgccagtc cacggttgct
     4501 ccgctttctt tagcaaaggt gttgtcggcc tgagccactt tcgccggttc ggctgaggtt
     4561 tgatacgcca cggtgacgtt caccgcctgt gcctgaaaag cgatgaatgc cagtgcggca
     4621 agaagtgtgt tacgcgatga aattgccatg attgtctgct cccctgtctt gttatgggag
     4681 cagtattcag gaataaaaac attcattaaa agaattagtc gttatcgcac agatgatttt
     4741 attcttagca aaaaaacggc taaggaaaga ttaaatatgc aattactccg ttctaacaat
     4801 tgggaaaatt actacacttc attttagtga tgcataaaca accacattac gagatttatt
     4861 acttcattat ttctatcaat atcaatacat ata
//
LOCUS       DAZGAN020000213         4800 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGAN020000213 DAZGAN020000000
VERSION     DAZGAN020000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.214"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(66..551)
                     /locus_tag="ACHKYO_RS24530"
                     /old_locus_tag="pgaptmp_004906"
     CDS             complement(66..551)
                     /locus_tag="ACHKYO_RS24530"
                     /old_locus_tag="pgaptmp_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4431587.1"
                     /translation="MTTVSHNFTTPSVSVTTASGNNQSQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCENYCDGIWNLYTLNNGGAFMAPEPDDDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMAYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(641..1459)
                     /locus_tag="ACHKYO_RS24535"
                     /old_locus_tag="pgaptmp_004907"
     CDS             complement(641..1459)
                     /locus_tag="ACHKYO_RS24535"
                     /old_locus_tag="pgaptmp_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4431588.1"
                     /translation="MRLASRFGYVNQIRRERPLTREELMYHVPSIFAEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHTLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLGCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPALQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETMLESMR"
     gene            complement(1550..1783)
                     /locus_tag="ACHKYO_RS24540"
                     /old_locus_tag="pgaptmp_004908"
     CDS             complement(1550..1783)
                     /locus_tag="ACHKYO_RS24540"
                     /old_locus_tag="pgaptmp_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGU4431589.1"
                     /translation="MPGCTSRLLPEGPFSRNQALAVTTAYLNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNPYLASEGILRQ"
     gene            complement(1854..4700)
                     /locus_tag="ACHKYO_RS24545"
                     /old_locus_tag="pgaptmp_004909"
     CDS             complement(1854..4700)
                     /locus_tag="ACHKYO_RS24545"
                     /old_locus_tag="pgaptmp_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HGU4431590.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNDGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDILESHSAQNTLVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGYRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIHFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVSELPVNWWVQPSVIRTFSSRGDMSMGTTAAGSNMTFSPSQNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSDSSAEGYNGQATLNVTF"
BASE COUNT          996 a         1402 c         1296 g         1106 t
ORIGIN      
        1 ctgcgctcgc cgggcgtcat ctctccgggc agaaaggaaa gctgctgcat tgtccctgcc
       61 tccattcagt cgatgatgcg cataatggcg ctgcattccg gatgctgcag ggcgtaatcc
      121 cgcagccggt aataatggac cgtcatggca taacactccg tacgacaggc gtgatggctg
      181 tacgccatca gacaggcggc aataccggca gcttccgggc tcatttcagc gcggttaccg
      241 ttcatggcat tgaacagtac ccatgtttct ccatcatcat catccggttc gggggccata
      301 aatgccccgc cgttgttcag ggtgtacaga ttccagatac catcgcagta gttttcgcaa
      361 agacggtcca tccagccgaa gatacggggc tccagggtga cccactgtgg aatgaggcca
      421 aagtgctgcg gccagaagct gatgcgctgt tcatcaggga ctatggtggc aaccaactga
      481 gactggttat tccctgatgc agtggttacg gaaacagaag gtgtggtgaa attatgcgag
      541 acggttgtca tgagattatt ccttataaaa agtaaatgaa tggaagaagt cgcgggccgg
      601 gacggacatg agtcagaact gcgctttcag ggaaaaggca tcagcgcatg ctctccagca
      661 tcgtttctgc catcacccac aacgcccggt tgagcttaat gtcggtatcg atgctgtgaa
      721 tggcccgggt atggatacgt tttcctctgg cactgcgacc ggaaatcccg cctttcagca
      781 tattttcctg gatggtctga taagcactcc acaggtcctt accgtaatcc tcccggcgtc
      841 gtggcgtcag aatgtcggca gtggtgacgg gctgatgttc atcaccataa cggtaagtca
      901 gcgccgcctg tgccagcgcc tggagtgccg gtggcggcag gaccagcgac tgcatggcat
      961 cacgcttttc ctcaatccgg tcaaacacgc ccaccacttc gtaagcacct tctatgaccc
     1021 tgtccaccac gtttccccgg tgtggcaccc ggacttctcc cagcgactga ccgcagccca
     1081 ggccattggt acaaatggca cgaaaatatc cgggtaacat ctggtagctg gatgaaccgt
     1141 catgggagtt gagcagaata atttcaggca catgctgacc ggttatctgt ccggcccgcc
     1201 gcagacgcag cgtgtgtttg gtatattccc ggcggctctg gtcgcgcaca cgggtctggc
     1261 aggcgaagaa cggctgaaag ccttcccgct gcagattttc caggacggtg atggtgggaa
     1321 tgtacgcata ccgttcactg cgggaggtgt gccggtcttc cgcaaaaata ctcgggacgt
     1381 ggtacatcag ttcttcgcgt gtcagcgggc gctcccggcg gatctgattt acataaccaa
     1441 aacggctggc taatcgcata attggctcct tatgaatgat taagcggtat aaatgaaaaa
     1501 gccgcgtctt tcggagaaga cgcggcctga tggatcaagt gaatgacgtt tattgcctga
     1561 gaattccctc actggcgaga tacggattta gctgttttcc ggcatcaggt tcaaaattcc
     1621 agcaccgcca gcgcaactgg ccttcagcgt tgcggatcac cagccggaaa tgcgttccct
     1681 ggtcatcttc aataagcaca ttaaggtaag ctgttgtgac ggcaagagcc tggttgcgtg
     1741 agaaagggcc ttcgggcaat aaacgtgatg tacaacccgg catatctgtt gctccctgta
     1801 agtaaaaggc cccggtcatg atgaccgggg cctgaagggg agtgacctga ttatcagaaa
     1861 gtcacattca gcgtggcctg accgttataa ccttcagcgc tgctgtcgct gacgctgtgg
     1921 gcataaccgg cctgaacgcc cagggtgata ttttcccgga cacgggcttc cagtccggcc
     1981 tgcaggtcca gtgacgtgcc attctgggac ggtgagaacg tcatgttact gccggctgcg
     2041 gttgtaccca tgctcatgtc accccgggag ctgaaggtgc ggataacaga aggctgtacc
     2101 caccagttca ccggcaattc actcacgctg tgttttgcac tgtcgcgcag ggtgtcacgg
     2161 gatgaggtgc ctttaccaaa gttcatatcg ttgtggctgc ccagacggaa gccggcacgc
     2221 acatgttgtg cactgccatg cccgaacttc acataaccgg cgttatcctg gccgtcatcc
     2281 agggagagcc cctgccaggt gtactgcagt tgtggctcca gcatcagatt gtcagtgata
     2341 ctgaagggca gaccggtttc cagtgagccc agccagcccc agccccgggc gcggaagtcg
     2401 ttattgtccg atgatgcttt catgctgtgg cgggttccct gtgccacaat gtcagcccac
     2461 aggccggagg atgtgtgtac cagattcagg tatccgccca gactgccggc atcatcccgg
     2521 accgtgccgg cgcgggagcc gtcatcatcc ttaacatcaa cggaagaatg gcctgcagca
     2581 ccatacaccc ctgtcgtcag agacataccg gcaacctctg ttctgagcag gtcaccctcc
     2641 agacggacga agccatagct gccgctgctt tccggcgtgg ccccacgggc aataccgccg
     2701 ttgttatcgt gcccgagatg accgccctga atgctgagac ggacgctgtt attttcaccg
     2761 tttacaccgg tctgatggct gcgggagcca gccagaatcc ggtcatagtc cattgcctgt
     2821 gtcagcatgg aggcatacag ggggacttca gcacgataag cattttcact gcgcaggtac
     2881 cagtcttcat cgctgtcacg gttcagggtg tagttaaagg cgccggcctg aagcgggcgg
     2941 ctcagggcaa acgcaccttc ttctgtggtg gcaccattct gtgcatccac aacccggata
     3001 ccctgtccgg tggttgccac cccgaggtta ctgtttccga catttgtgaa cgcaagccag
     3061 gttttgccgg ttgcctggcc accattaatc accagctggt cagaggcatt gctgccatca
     3121 aggcgaacac gcatattgat ggtgcccccc tgaccggtga ggttactggt ggtcagttta
     3181 tggaacgtta ccggggagtc gccttttgca acagcacggc tcagcgcggc atccggtgtc
     3241 gtctgattat caaagtgaat ctctcctgcg ttaacaatat cgccggtact gaccacatca
     3301 ccattcagta ccatgctggc gcctttactg agctgtgttc tgccactcag acttcccccg
     3361 gcagccagcg tcagtgttcc acgatatccg acagtggtgt taccggcctg tcccccggca
     3421 ttaaccgtaa agctgccgcc attttccagc agcaggccgc tggcctgacc ggagatacca
     3481 tcaatactga acttaccgct ggcattggtc ccctcaacag tggcaccact gtctgcaatc
     3541 agggcaccac cggatgtcat ggtgacacct gtcgccttac cgccggcaga cactgccagg
     3601 gtaccgccgt catccaccag ggtattctgt gctgaatggc tctccagtat atccagacga
     3661 ccgccggatt ccagaacaac accgtcagcc ttaccgtttt ccaccgtgaa attccccaga
     3721 cggttgctgc caagaacagt tgctgcagta ctggtgacca gtgcgcctcc ctgcttcagg
     3781 gtgacattcg tggctgtacc accggcgttc acctgcagtt tgcctttctg gttaacggtg
     3841 gtgaaatccg ccagaccacc ttccttgata atctgccagc cgtcactgtt tacaacagtg
     3901 ccagacgccg taccgtcgtt gtgcacatac tggtaccccc cattcagcgt ggtatccagt
     3961 gcgtgaccat gtacagtctg gtcgccgccg gcataaacca cagtggtatt tgccgttcct
     4021 tcagcagcca caatctgacg accattttta ttgatggtgg tgcgtacggc atctccgtag
     4081 acggtctgcc cggtatcacc attttccgca tccggtcccc cttccgcgcc ggtattcaca
     4141 accgtgtcgg ttgccactgc gccggatttg atggcctgcc agcccttctc attaatgacg
     4201 gtacccgttg caatcccgcc ttcatgtacc cactgctcac cgccgttcag cgtggtgttc
     4261 actgcctgcc cctgaaggct ctgtccgcct ccggcactga taaccgtgtc tgaaacgctt
     4321 cctccggcat tcactctctg aagaccacca ccggtgacag tagtgttgtt ggcgataccg
     4381 ccattttgta tccattgtcc gccggtattg gcctcgttat ccggcccata ctccagcccg
     4441 gtactgatgg tcattccgtt ggccgtaccg aggacaatct ggttgtcatg atttgtcagt
     4501 gttccgtcgt tcacggtttc tcccgcctgt acaaccgtgt cagcagccag tgccgggact
     4561 gatgtgacag cagcaagaga cagtgcaacc gccacaccgg cgcgttttcc ccgtgagcgg
     4621 gccagttcgg aggccaccac cagggtgccc gtaatgtgat tccataccag cctgtagctg
     4681 gtgttcagat gtcgtttcat cagcttttcc ttacagaggg tgaataaaaa agccggtacc
     4741 cacaaatgtg gataccggca ggtacaaaac agcatggtca gatacagtgg tttttcccgg
//
LOCUS       DAZGAN020000214         4791 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGAN020000214 DAZGAN020000000
VERSION     DAZGAN020000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.215"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            67..351
                     /locus_tag="ACHKYO_RS24550"
                     /old_locus_tag="pgaptmp_004910"
     CDS             67..351
                     /locus_tag="ACHKYO_RS24550"
                     /old_locus_tag="pgaptmp_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4431591.1"
                     /translation="MRELVNQHNHGIQPVITPVVQINANEWVTLELLMAVTGLRKGTI
                     LRARDSAWMNGREYKQIAPDGTPKKNSECLYHLPTINTWIKNQPLPSQDV"
     gene            404..1714
                     /locus_tag="ACHKYO_RS24555"
                     /old_locus_tag="pgaptmp_004911"
     CDS             404..1714
                     /locus_tag="ACHKYO_RS24555"
                     /old_locus_tag="pgaptmp_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4431592.1"
                     /translation="MANSAYPAGVENHGGKLRITFKYRGKRVRENLRVPDTPKNRKIA
                     GELRASVCFAIRTGTFDYAERFPDSPNLKLFGLVKKDITVGELAQKWLTLKAMEISSN
                     ALNRYQSVMKNMLPRLGPGRLASSITKEDLLFIRKDLLTGEKGSRKTSTSQKGRTVPT
                     VNYYMTTTAGMFSFAAENGYLEKNPFNSITPLRKSKPVPDPLTRDEFSRLIDACHHQQ
                     TKNLWTVAVFTGMRHGEIAALAWEDIDLKAGTITVRRNFTKIGDFTLPKTDAGTNRVI
                     HLLAPAIEALKNQAMLTRLSRQHQITVQLREYGRTILHECTFVFCPQIVRKNHKAGIN
                     YAVSSIGATWDSAIKRAGIRSRKAYQSRHTYACWALSAGANPTFIASQMGHSSASMVY
                     NVYGAWMPECSVTQVAMLNNVLNARAPDVPQSDQEDEIKLYFSK"
     gene            1711..2289
                     /gene="wrbA"
                     /locus_tag="ACHKYO_RS24560"
                     /old_locus_tag="pgaptmp_004912"
     CDS             1711..2289
                     /gene="wrbA"
                     /locus_tag="ACHKYO_RS24560"
                     /old_locus_tag="pgaptmp_004912"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU4431593.1"
                     /translation="MINHILRHVETMARAVAEGASKVDGAEVVVKRVPETMPPQLFEK
                     AGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLA
                     SVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVTQVRGGTPYGATTIA
                     GGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            2310..2537
                     /locus_tag="ACHKYO_RS24565"
                     /old_locus_tag="pgaptmp_004913"
     CDS             2310..2537
                     /locus_tag="ACHKYO_RS24565"
                     /old_locus_tag="pgaptmp_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HGU4431594.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(2575..3816)
                     /gene="agp"
                     /locus_tag="ACHKYO_RS24570"
                     /old_locus_tag="pgaptmp_004914"
     CDS             complement(2575..3816)
                     /gene="agp"
                     /locus_tag="ACHKYO_RS24570"
                     /old_locus_tag="pgaptmp_004914"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HGU4431595.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGQLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDKQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(4110..4791)
                     /locus_tag="ACHKYO_RS24575"
                     /old_locus_tag="pgaptmp_004915"
                     /pseudo
     CDS             complement(4110..4791)
                     /locus_tag="ACHKYO_RS24575"
                     /old_locus_tag="pgaptmp_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535347.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1335 a         1150 c         1093 g         1213 t
ORIGIN      
        1 cgccgtacat caatcaactg acaaccgcct tcgggcggtt ttttcttaca catgcagagg
       61 caacttatgc gcgagttagt aaaccaacat aaccatggca ttcagccagt catcacacct
      121 gttgtacaga taaatgcgaa tgaatgggta acactggagc ttttaatggc tgtaacaggc
      181 ctaagaaaag gaacaatatt acgcgccagg gacagtgcgt ggatgaacgg cagagaatat
      241 aaacaaattg cccccgacgg aacgccaaag aaaaacagcg aatgtctcta tcatcttcct
      301 accatcaaca cttggatcaa aaaccaaccc ttaccatctc aggatgttta attcttgtcc
      361 ataagagtat aacctgagcg tgctcttgga cgcaggagga acaatggcga attcagccta
      421 tccagccggc gttgaaaatc acggaggaaa actccgaata acgtttaagt acaggggtaa
      481 acgagtgcgc gaaaatcttc gcgtgcccga tactccgaaa aacagaaaga tcgctggtga
      541 gttaagggct tctgtctgct ttgcaatcag aacgggaacg tttgattatg ccgagcgatt
      601 ccctgactca cctaacctga agctatttgg cctggtaaaa aaagatatca ccgtcggtga
      661 actggcacag aaatggctta ctctgaaagc aatggaaatc agtagtaacg ccttaaatcg
      721 ttatcaatca gtgatgaaaa atatgctacc gagacttggt cctggcaggc tggcgtcatc
      781 gattacaaaa gaagatctgc tgtttatcag gaaagattta ctgaccgggg aaaagggaag
      841 caggaaaacc agcacgtccc aaaaaggaag aaccgtaccc acagtgaact attacatgac
      901 aacaacagcc ggaatgttca gctttgccgc cgaaaacggg tatctggaga aaaacccgtt
      961 taattcaata acaccgctga ggaaatcaaa accagtgccg gatccactga ccagagatga
     1021 gtttagccgt ctcattgatg cctgccatca tcaacagacc aaaaacctct ggacagtggc
     1081 tgtttttaca gggatgcgac acggtgaaat tgccgcactt gcatgggagg atatcgacct
     1141 gaaagctggc acgataacag tgcgaagaaa ttttacaaaa ataggtgatt ttacgctacc
     1201 aaagactgac gcaggcacta accgggttat acatcttctg gcaccagcaa ttgaagcact
     1261 taaaaaccag gcgatgctta ctcgtcttag caggcagcat cagatcactg ttcaattacg
     1321 cgagtacgga agaacaattt tgcacgagtg cacttttgtt ttctgtccgc aaatcgttcg
     1381 caagaatcac aaggctggta ttaactacgc ggtaagctcc atcggagcga catgggattc
     1441 agcaataaaa agagcgggta tccgatcccg taaagcgtat cagtcacgcc atacctatgc
     1501 gtgctgggct ttatctgccg gagcaaaccc gacatttatt gcatcacaga tggggcactc
     1561 cagcgccagc atggtctaca atgtttatgg tgcatggatg cctgagtgca gcgtgactca
     1621 agttgccatg ttgaataatg tccttaatgc ccgtgcccca gacgtgcccc aaagtgacca
     1681 ggaggatgaa ataaaattat atttttcaaa atgataaacc atatccttag acatgttgaa
     1741 acgatggcac gcgcagtcgc tgagggtgca agcaaagtcg atggcgcaga agttgtcgtt
     1801 aagcgtgtac cggaaaccat gccgccgcaa ttatttgaaa aagcaggcgg taaaacgcaa
     1861 actgcaccgg ttgcaacccc gcaagaactg gccgattacg acgccattat ttttggtaca
     1921 cctacccgct ttggcaacat gtccggtcaa atgcgtacct tcctcgacca gacgggcggc
     1981 ctgtgggctt ccggcgcact atacggaaaa ctggcgagcg tctttagttc caccggtact
     2041 ggcggcggtc aggaacaaac tattacttca acctggacga cccttgcgca tcacggcatg
     2101 gtaattgtcc ccattggcta cgcagcgcag gaattatttg acgttacaca ggttcgcggc
     2161 ggtacgccgt acggcgcaac caccatcgca ggcggtgacg gctcacgcca gccaagccag
     2221 gaagaactgt ctattgctcg ttatcaaggg gaatatgtcg caggtctggc agttaaactt
     2281 aacggctaat cttcaacagg aggatacgca tgccaactca agaagcgaaa gctcatcacg
     2341 tcggtgaatg ggcatctctg cgcaatacgt cgccggaaat agccgaggcc attttcgaag
     2401 tcgccgggta cgacgaaaaa atggcggaaa agatttggga agaaggtagc gatgaagtgt
     2461 tagtcaaagc ctttgctaag accgataaag attcgctttt ttggggcgaa cagaccatcg
     2521 aacgtaaaaa cgtttaacca gcgactcccc cgcttctcgc gggggagttt tctgttattt
     2581 caccgcttca ttcaacacgc tatcaaattt atccatcggg cagaaaccgt tggcatctat
     2641 cgggcaaccg cttaattcca gcgtcacacg ctgagcaggt gcctgcaggg ttaacgcatc
     2701 ggcattacgt aactgttccg cactctgata cacatattca attttcatca aatcgcgatt
     2761 ggctttgctg tcatgccaac gctggaaaac gattttgccg ccaatcggcg tgcgttcgtt
     2821 ctggtcatgc aactgatacg gtttgaaatc cagcgccgtt aacagagagg caatgttgga
     2881 gtcgtgccca accaacactg taattttcgg tgcgctggtg cgatcggtga ccagagcttt
     2941 gtcgatataa ctgaccagcg gtttcgcaac attgcgcgcc acttccggtg aggtaaacag
     3001 gctgtcctgg tagccgtttt taagcttcga caacaccttc cactgcttgt cagatttgat
     3061 ttctccccag gccacctgat ccatcggaaa accttcgtaa tattgcaaag taaacgcatc
     3121 taccagcgag ttgccgactt tcagctgccc ggaaacacct ggttcttgtt gatacttggc
     3181 gctaaaggta tttttgccat ccaccagcga acactgttgt ttctctttac aggcagggga
     3241 atctttatag ttaacgattt tttccagtag ctggtagctg tcggtaagct ggagtttgct
     3301 gagctctttc tccattgccg ccaccgcctg ttcactgaat gcggcggaat catcggtgat
     3361 caccgggtta aaggttgggt ccatagtgcc cattttttcc tggtgatgca caggaatatc
     3421 acaccccggg aatgcgccgg taataaagaa ctgtgcggtc gcaacggtac gttgcagact
     3481 attggcatag gcataaacgg tgtccggcgg cgggcattcc cccgatttca ccatcccctg
     3541 ctgtgccagc cattcacgca tgtaatggcc catatacact tcgagcacgc cacctttggt
     3601 ggtgagttgc ccaccgggga cgtcccattc gggccattta ttcggcgtag actgctccag
     3661 cacactgcca ttgttcgcca gcggcgcacg taagttatgg cggctcatca tgagcacttg
     3721 ctgtagctga tagccttccg gtacggtttg tgcctgagca tttgaagcga gtaaaactat
     3781 ccctgccaca gttgcggcga ttagcgtttt gttcattcct ggcacctctt ttgttatcaa
     3841 tcactaaaga gtgtgacaga aatatgacat ttttccggga acaggttcgc aaaatggctt
     3901 gttgaatgcc tgtttttcta tatacatctt taaaaacagg caaggtttga cgcaaactat
     3961 gcctgattag ctccctgcct cgcgtacgcc gctgatgatt acacctgtgg ctaaacagaa
     4021 tatctgcggc gttaccaata cagagacaat atttaccaat aaaaaagtgt gagactaata
     4081 acaatggttt ttagcatatg cctttgccat taaattgcat aacgatttaa atagtcgagt
     4141 tgcgcattca tttccttatt ttttaactta taagcctgag caaaaagttc aggagggagg
     4201 taaacatcat aatgattgcc agcgtttatt aaataaccat actcaaggtc gtcatctgtt
     4261 gaaaaggatt cattccccaa ggagattgat gccatattac tattaacaat aaataatgag
     4321 caaatgctat ctgaaagagc agtgaggaaa tttttcacct cattaaccct ctgctctggg
     4381 tcggtgtcta aaacaaggcg taccttgtta tcattaaata taaattcggg taaatgcagt
     4441 atgcattttt tgtcattcaa gcaactatca ataaattctt ttttaaaaga caaaaaactc
     4501 catgataaaa aaccaccctg ccctgaattt tgttgtcaga ttgtagatta acgccaaata
     4561 acgtctttac atcttccctg agattataga atgccgtatc aacaagagcc tcgatcgttt
     4621 gtttatcaac aattttaata aactcttcgt atacttgctt ttgcgtctta tggccgccca
     4681 gcgcccatga atactcatgt tcttttaaaa ccagtatggc tcttaacaga caatatccat
     4741 cgccatctac tggagcgtat tttaatccat atgttttatt aatttcactc t
//
LOCUS       DAZGAN020000215         4603 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGAN020000215 DAZGAN020000000
VERSION     DAZGAN020000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.216"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..309)
                     /locus_tag="ACHKYO_RS24580"
                     /old_locus_tag="pgaptmp_004916"
     CDS             complement(<1..309)
                     /locus_tag="ACHKYO_RS24580"
                     /old_locus_tag="pgaptmp_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU4431596.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     I"
     gene            complement(424..499)
                     /locus_tag="ACHKYO_RS24585"
                     /old_locus_tag="pgaptmp_004917"
     tRNA            complement(424..499)
                     /locus_tag="ACHKYO_RS24585"
                     /old_locus_tag="pgaptmp_004917"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(464..466),aa:Thr,seq:ggt)
     gene            complement(506..580)
                     /locus_tag="ACHKYO_RS24590"
                     /old_locus_tag="pgaptmp_004918"
     tRNA            complement(506..580)
                     /locus_tag="ACHKYO_RS24590"
                     /old_locus_tag="pgaptmp_004918"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(545..547),aa:Gly,seq:tcc)
     gene            complement(697..781)
                     /locus_tag="ACHKYO_RS24595"
                     /old_locus_tag="pgaptmp_004919"
     tRNA            complement(697..781)
                     /locus_tag="ACHKYO_RS24595"
                     /old_locus_tag="pgaptmp_004919"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(745..747),aa:Tyr,seq:gta)
     gene            complement(790..865)
                     /locus_tag="ACHKYO_RS24600"
                     /old_locus_tag="pgaptmp_004920"
     tRNA            complement(790..865)
                     /locus_tag="ACHKYO_RS24600"
                     /old_locus_tag="pgaptmp_004920"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(830..832),aa:Thr,seq:tgt)
     gene            1227..2177
                     /gene="coaA"
                     /locus_tag="ACHKYO_RS24605"
                     /old_locus_tag="pgaptmp_004921"
     CDS             1227..2177
                     /gene="coaA"
                     /locus_tag="ACHKYO_RS24605"
                     /old_locus_tag="pgaptmp_004921"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGU4431597.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2206..3171)
                     /gene="birA"
                     /locus_tag="ACHKYO_RS24610"
                     /old_locus_tag="pgaptmp_004922"
     CDS             complement(2206..3171)
                     /gene="birA"
                     /locus_tag="ACHKYO_RS24610"
                     /old_locus_tag="pgaptmp_004922"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGU4431598.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEEILSQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3168..4196)
                     /gene="murB"
                     /locus_tag="ACHKYO_RS24615"
                     /old_locus_tag="pgaptmp_004923"
     CDS             complement(3168..4196)
                     /gene="murB"
                     /locus_tag="ACHKYO_RS24615"
                     /old_locus_tag="pgaptmp_004923"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGU4431599.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTHLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4499..>4603)
                     /gene="rrf"
                     /locus_tag="ACHKYO_RS24620"
                     /old_locus_tag="pgaptmp_004924"
     rRNA            complement(4499..>4603)
                     /gene="rrf"
                     /locus_tag="ACHKYO_RS24620"
                     /old_locus_tag="pgaptmp_004924"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1159 a         1161 c         1049 g         1234 t
ORIGIN      
        1 gatcgcgccg tccatctgag cagcaccggt gatcatgttt ttaacatagt cggcgtgccc
       61 cgggcagtct acgtgtgcgt agtgacgggt cggggtgtcg tattcaacgt gagaagtgtt
      121 gatggtgata ccacgagctt tttcttccgg cgcgttatcg atctggtcga atgcgcgagc
      181 agcaccgccg taggttttag ccagtacggt agtgattgca gcggtcagcg ttgttttacc
      241 atggtcaacg tggccgatag taccgacgtt aacgtgcggt tttgtacgtt caaacttttc
      301 tttagacatc gattgtccct ctaagacacg gataaatcgg tgatatcacc acatcaacca
      361 ggcaacatgc ccgacttgtt gaatgcaata aacagaagga aaaaacaggg aggagaaaag
      421 aagtggtgct gataggcaga ttcgaactgc cgacctcacc cttaccaagg gtgcgctcta
      481 ccaactgagc tatatcagca catcttggag cgggcagcgg gaatcgaacc cgcatcatca
      541 gcttggaagg ctgaggtaat agccattata cgatgcccgc atcctggaac tcggctacct
      601 gattttcatt ctgcactgaa tatcgagaga agctctcttt attcgagccg gtaagcgaac
      661 ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt ggtgggggaa ggattcgaac
      721 cttcgaagtc tgtgacggca gatttacagt ctgctccctt tggccgctcg ggaaccccac
      781 cggacttgat ggtgccgact accggaatcg aactggtgac ctactgatta caagtcagtt
      841 gctctaccta ctgagctaag tcggcatcaa gtagcgcgca ttctatggag acatgcgagt
      901 tcatgcaact aaaaaattgc ataatttgtt ttattggtca cattttatgc gacacgatga
      961 agaaacagcc gaacggaggg cagattagca cactttttca acatcattgt gctcaacaat
     1021 gcgctcctgc taaaccataa ttctttttat cagatggaat atctgtcaca ttgcttttca
     1081 acgatagctt cctggcagag attttttctt attattcctc cccatctggt gttaccctcc
     1141 tgcccattaa cccattcaac agaactgtga cgcgccatgg caaatatcgc tttgccgata
     1201 gagctatgac cgccagaaac atgcttatga gtataaaaga gcaaacgtta atgacgcctt
     1261 acctacagtt tgaccgcaac cagtgggcag ctctgcgtga ttccgtacct atgacgttat
     1321 cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga tctctcgtta gaagaagttg
     1381 ccgagatcta tttacctttg tcacgtttgc tgaacttcta tataagctcg aatctgcgcc
     1441 gtcaggcagt tctggaacag tttcttggta ctaacgggca acgcattcct tacattatca
     1501 gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc ccgtgtattg caggcgctat
     1561 taagccgttg gccggaacat cgtcgtgttg aactgatcac tacagatggc ttccttcacc
     1621 ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa aggcttcccg gaatcgtatg
     1681 atatgcatcg cctggtgaag tttgtttccg atctcaaatc cggcgtgcca aacgttacag
     1741 cccctgttta ctcgcatctt atttatgatg tgatcccgga tggagataaa acggttgttc
     1801 agcctgatat tttaattctt gaagggttaa atgtcttaca gagcgggatg gattatccac
     1861 acgatccaca tcatgtattt gtttctgatt ttgtcgattt ttcgatatat gttgatgcac
     1921 cggaagactt acttcagacg tggtatatca accgttttct gaaattccgc gaaggggctt
     1981 ttaccgaccc ggattcctat tttcataact acgcgaaatt aactaaagaa gaagcgatta
     2041 agactgccat gacattgtgg aaagagatca actggctgaa cttaaagcaa aatattctac
     2101 ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc taatcatgcg gtagaagagg
     2161 tcagactacg caaataattt tcaggggagc taatgctccc ctttcttatt tttctgcact
     2221 acgcagggat atttcaccgc ccatccaggg ttttattatt ccatcctgct caagcaataa
     2281 agcgccctgt ttgtctattc cgcgtgaaat gccaaatatt tctttatcac caatgataag
     2341 tttcactggg cgattaataa aattatccag cttttcccag cgcgaaagat aaggtgccaa
     2401 tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca cgtattagca tggccgccaa
     2461 cgtattacga tcgagattga tccccgcttc ctgcagcgtg atccacccct gattaacgac
     2521 actctcttca acacgacgca ttgccatgtt gatcccggct ccaatgacta tttgcgccgc
     2581 atcgccggtt ttgccagtca gctccacaag aatgcctgcc agcttgcgat cctgcagata
     2641 gaggtcatta ggccatttaa cacgaacttt atctgcaccc agcttgcgta atacttccgc
     2701 catcacgata ccgataacca gacttaaacc aatagccgcc gccgggcctt gttccaaacg
     2761 ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa aaccatttcc gaccccggcg
     2821 accacggcca gcctgctggt attctgcaac gcaggcatcg cccgatttaa gctctccgat
     2881 acgatcaaga aggtactgat tcgtggagtc aatcactggc agcacggcta cactaccgcc
     2941 atccagctga ctcaatatct cttcagcatt aagtaactgg ataggttcag gcaggctgta
     3001 tcctttaccc ggaacggtaa agacatcaac gccccagtca cgcagtgtct gaatgtgttt
     3061 attaatagcc gcccggctca ttcccagcgt ttcacccaac tgctcgccag agtgaaattc
     3121 accgttcgct aacagggcaa tcaatttcag tggcacggtg ttatccttca tgaaattgtc
     3181 tccactgcgc tcacttcacc tgatgcacca ataaagcgga cttcaggctc aagccagaca
     3241 ttaaattttt cacccacttt ttgtcttaca tggtgtgcca gttgcaccac atcttcgctt
     3301 tttgcattgt cttcattaat gagaactaac gcctgttgac ggtgcaccgc agccccaccc
     3361 atttgcatcc cttttagctg gcactgatca ataagccaac ctgctgccag ttttactgaa
     3421 ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg ccagtaatgc tttagccgtt
     3481 tcggcagata caacagggtt tttgaagaaa ctaccggcat tgccattcac ttttggatca
     3541 gggagtttgg tggtgcgcat atgacacacc gcattaaata cttgctgtgg cgttactgtt
     3601 gtaggatcca gatgagttaa gtcaccatac gttagtacag gttgccactc ttttggcaga
     3661 cgcagaccta cggctacaat ggcgaagcga tcctggtatt catgtttaaa aatactgtcg
     3721 cgatagccaa aacggcactc tttggcagtt aagcgcactt gcttgcctgt cgccagttca
     3781 acacaatcaa cataagcgca aactcgctgt aattctacgc cataagcacc aatattctgg
     3841 ataggtgatg agccgacaca accaggaatt aatgccagat tttccagacc aggcatacct
     3901 tcctgcaaag tgtattttac caggcgatgc cagttttctc cggctcctac atgtaaatac
     3961 cacgcatcag gttcatcatg aatttcgata cctttgatcc ggttgatgat caccgtgccg
     4021 cgatagtctt ccagaaaaag tacattactt ccttcaccca gaataagaac gggttgtcct
     4081 tctgcggttg catactgcca ggcattgagt aattgttgtt cgtcttcggc acatacaatg
     4141 tgctgagcat tatgatcaat gccaaatgtg ttccagggtt ttaaggagtg gttcatagct
     4201 gctttcctga tgcaaaaacg aggctagttt accgtatctg tggggggatg gcttgtagat
     4261 atgacgacag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
     4321 ttaatttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
     4381 ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
     4441 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc
     4501 ctggcagttc cctactctcg catggggaaa ccccacacta ccatcggcgc tacggcgttt
     4561 cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg cta
//
LOCUS       DAZGAN020000216         4507 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGAN020000216 DAZGAN020000000
VERSION     DAZGAN020000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.217"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..666
                     /locus_tag="ACHKYO_RS24625"
                     /old_locus_tag="pgaptmp_004925"
     CDS             <1..666
                     /locus_tag="ACHKYO_RS24625"
                     /old_locus_tag="pgaptmp_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HGU4431600.1"
                     /translation="LPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGY
                     QLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQ
                     SICEDKEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSH
                     GEYWDEIFSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDNHGKEITNRKGLPNVCRVH
                     GNM"
     gene            638..1021
                     /gene="yhhH"
                     /locus_tag="ACHKYO_RS24630"
                     /old_locus_tag="pgaptmp_004926"
     CDS             638..1021
                     /gene="yhhH"
                     /locus_tag="ACHKYO_RS24630"
                     /old_locus_tag="pgaptmp_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HGU4431601.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
     gene            1126..1968
                     /gene="yibA"
                     /locus_tag="ACHKYO_RS24635"
                     /old_locus_tag="pgaptmp_004927"
     CDS             1126..1968
                     /gene="yibA"
                     /locus_tag="ACHKYO_RS24635"
                     /old_locus_tag="pgaptmp_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HGU4431602.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMALND
                     KSACVRATAIESTAQRCKKNQIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKAT
                     IPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSY
                     RKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDK
                     LKRS"
     gene            1996..2954
                     /locus_tag="ACHKYO_RS24640"
                     /old_locus_tag="pgaptmp_004928"
                     /pseudo
     CDS             1996..2954
                     /locus_tag="ACHKYO_RS24640"
                     /old_locus_tag="pgaptmp_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            2966..3427
                     /gene="yibG"
                     /locus_tag="ACHKYO_RS24645"
                     /old_locus_tag="pgaptmp_004929"
     CDS             2966..3427
                     /gene="yibG"
                     /locus_tag="ACHKYO_RS24645"
                     /old_locus_tag="pgaptmp_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGU4431603.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDTKSKLQELKKLKRMDRRKNKENP"
     gene            4062..>4507
                     /locus_tag="ACHKYO_RS24650"
                     /old_locus_tag="pgaptmp_004930"
     CDS             4062..>4507
                     /locus_tag="ACHKYO_RS24650"
                     /old_locus_tag="pgaptmp_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HGU4431604.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASNKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDKNKKIIDINSVNICSDDKN"
BASE COUNT         1509 a          752 c         1002 g         1244 t
ORIGIN      
        1 ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
       61 gacccgctgc aggggcggta tatcactcag gatccgattg ggctgaaggg gggatggaac
      121 ctgtatggat atcaattgaa tccgatatca gacatcgacc ccctgggttt atctatgtgg
      181 gaggatgcaa aatcgggggc atgtactaat ggtctttgcg gcacactatc cgctatgata
      241 ggtccagata aatttgattc tatagatagc accgcatatg acgccttaaa taaaataaat
      301 agccaatcta tttgcgaaga taaagagttc gctggtttaa tatgtaagga taatagtggc
      361 agatatttct caacagcacc taaccgagga gaaagaaaag gatcatatcc attcaatagc
      421 ccttgcccta atggtactga gaaagtatca gcttatcata ctcatggtgc agatagtcat
      481 ggagaatatt gggacgaaat attttcaggt aaagatgaga aaatagttaa aagtaaagat
      541 aacaatatca agtcatttta tttaggtacg cccagtggta attttaaagc aatagataac
      601 cacgggaagg aaataacaaa cagaaaagga ttacctaatg tctgcagagt tcatggtaat
      661 atgtaaaaaa atattgttta ggaactgtgt cattgtatct ttgtttgttt ttacatacaa
      721 cacatgggcg cagtgtaata ataatattaa aataatgcgc aagtatgaaa gtgaaggtaa
      781 atataccgtt agaaatttgg ttaaaaataa agctatagca ttggaattag ctgagatata
      841 tgttaagaat cgttatggac aggatgccgc agaagaagaa aaaccatacg aaatcactga
      901 gttaacaaca agttgggttg ttgaaggtac cattcactca gaccaaattg ctggtggggt
      961 ttttattata gaaataggca aaaatgatgg gagaattctg aattttggcc acggaaaata
     1021 agtatgtaag gataatcaaa catcgtgtgc gctgatggca gagtaggtgg aggactccag
     1081 acagtcaaac gatagaaaaa gatagccttt atggaggttc ctgcaatgtc aaatacatac
     1141 cagaaaagaa aggcaagtaa agagtatggt ttatataatc aatgtaagaa actaaatgat
     1201 gatgaattat ttcgcttact tgatgatcac aattccttga aaaggatttc atctgccaga
     1261 gtattacagt taagaggtgg gcaagacgct gttagattgg caattgagtt ctgctctgat
     1321 aaaaattata tccgtagaga tatcggagca tttatactcg ggcaaataaa aatttgcaaa
     1381 aaatgcgaag ataatgtttt taatattttg aacaatatgg cattgaatga taagagcgct
     1441 tgcgttcgag ctacggcaat cgagtcaacg gctcagcgat gcaagaaaaa ccaaatttat
     1501 tcacctaaaa tagtagaaca atctcaaatt actgcttttg ataaatcgac taatgtcaga
     1561 cgtgctacag catttgctat ttctgttatc aatgataaag caacaattcc actattgatt
     1621 aatctgttaa aagatccaaa tggagatgtc agaaactggg ccgcatttgc aataaatatc
     1681 aataaatatg ataatagtga tattagggat tgttttgtgg agatgcttca ggataaaaat
     1741 gaggaagtcc gtattgaagc aataatcgga ctttcctaca gaaaagataa aagggtttta
     1801 tctgttttat gcgatgagtt aaaaaaaaat actgtttatg atgatatcat tgaagctgcg
     1861 ggtgaattag gtgataaaac gctacttcct gttttagata ctatgttgta caagtttgat
     1921 gacaatgaaa ttataacttc cgctattgat aagctgaagc gttcatagcg tgagttgcct
     1981 atgcacagtg ggggattccc gccggcacgg tgcagatgct cgaccggctg gaaagtgaaa
     2041 tcctggctga ccgggtgagt gaggaaagcc gccgctggct ggcatcgtgc ggcctgaccg
     2101 tggagcagat gaaaaaccag atggacccgg tgtacacgcc ggcgcgaaaa atccacctgt
     2161 accactgcga ccatcgcggc ctgccgctgg cgctcatcag cacggaaggg gcaacagcgt
     2221 ggtgcgcaga atatgatgaa tggggcaacc tgctgagtga tgagaacccg catcatctgc
     2281 agcagctcat tcgcctgccg ggtcagcagt atgatgagga gtccggcctg tattacaacc
     2341 gccaccgcta ttatgacccg ctgcaggggc ggtatatcac tcaggatccg attgggctga
     2401 aggggggatg gaatttttat cagtatccgt tgaatccggt cataaatgta gatccgcaag
     2461 gtttggttga tataaattta taccccgaaa gtgatcttat ccattctgta gctgatgaga
     2521 ttaatatccc aggcgttttc acaatcgggg ggcatggtac ccccacatct attgaatccg
     2581 caacgcgcag tatcatgaca gctaaagatc tagcatatct aattaaattt gatgggaatt
     2641 ataaagatgg gatgacagtt tggttatttt cttgtaatac aggtaaagga caaaactcat
     2701 ttgctagcca attagctaaa gagttacata caaatgtaat aggacctgac acgctatgga
     2761 cgtggtgggg gcgaggaact aatggtaagt taaaaatgga tacagtgcta acagcaccaa
     2821 cgaaccttaa ttcaaataag gatctaatgg ctataacaac aaaagacctt ggtaattgga
     2881 taacatatgg gccatctggg caccccattt ctaatatgca aggtacgcca gaaaaaccca
     2941 gtgatataag ataggttgta gatgtatgaa agcatgcttg ttactatttt tttatttttc
     3001 tcttattggt caattgcatg gtgctgatgt gaaaataaaa gaaaacgaaa gtgtgatggg
     3061 atctacagca atgacctatg atttaagtga agaaaagctg atgaaactga agtataaatc
     3121 acaacatggt gattcagagg catcatttcg actatatcaa tattactgct ttactaaaaa
     3181 taatattgat aaacaattgc gattcttgga aagatcagca tctcagggga atgttacagc
     3241 gcagtttaat tatggggtct ttttatcaga tacaaatcca acattatcag aatattataa
     3301 tttgaataga gctatttatt ggatggaatt cgctgtaaat aacggtaata ttgatacaaa
     3361 gagcaaactt caagagctca aaaagctaaa gcgaatggat agaaggaaga ataaggagaa
     3421 tccgtaaaag caatagttca tcagactgcc gcagcagtag tatgatgatg aatcagggct
     3481 gtactataac tattatcggt aacattaacc gcagcagggg cggtataaca ctcaggctcc
     3541 gattgggctg gaaggtaggt ggaattttta tcagtatccg ttgaacccga tttctggaat
     3601 cgatcccctt gggattagcc acctgtttat acagtataac tttaggtatg ctttcgtgtg
     3661 tttcagatat accatgtgat gataattttt atgatgtgct tagtattcca gtcgcttctg
     3721 gtaataatat gcaatgtaaa aataatcccc gttgtacaca tttataaata gatggccaat
     3781 acctcaaggc ctatggagtt ggaatgttaa tgacccggga gcaagcaata gaaaacctga
     3841 cggcatacga ttagtaccgt cagttaatac aggaacttat aatcgcaatg gattttcaat
     3901 acatagttgt ttaaacgctt ttggtccgtc tttaggacct cgattctgtt ccgagggttg
     3961 tataactggt ttatctaatg acatgcaaaa attaaatgag ttaatatttt cagagccaga
     4021 tagcacatta acagttacgg actaaaaaat atggcaaggt tattaacaat tgtatttttt
     4081 tctctatttc tgcgagtgca gttactgcaa gacaatttca gagagagtta gatttggcag
     4141 acaaagcaat attatgggca ctaatatcag ccagtaataa agagggacga aaagcgtgtt
     4201 cactaagcta ttttgcctgt aaggccgcag aggctgaact cggactggca tatatggctg
     4261 cgaatgataa caaggaattt ctcacatcct tatctaatat aatgaggtac aaaatagatg
     4321 ctggactttc cgaatcctat acatgctatt tattaagtaa gggaaagatc ataagaccat
     4381 atctgaaaaa tctaaatcca ctccaacttg ccgctgattg cattgaaaca gtaaataaaa
     4441 taaaagataa gaataaaaaa atcattgata taaattcagt taatatttgt agtgatgata
     4501 aaaatat
//
LOCUS       DAZGAN020000217         4503 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGAN020000217 DAZGAN020000000
VERSION     DAZGAN020000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.218"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            265..1422
                     /gene="acrE"
                     /locus_tag="ACHKYO_RS24655"
                     /old_locus_tag="pgaptmp_004931"
     CDS             265..1422
                     /gene="acrE"
                     /locus_tag="ACHKYO_RS24655"
                     /old_locus_tag="pgaptmp_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HGU4431605.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1434..>4503
                     /gene="acrF"
                     /locus_tag="ACHKYO_RS24660"
                     /old_locus_tag="pgaptmp_004932"
     CDS             1434..>4503
                     /gene="acrF"
                     /locus_tag="ACHKYO_RS24660"
                     /old_locus_tag="pgaptmp_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HGU4431606.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPTSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNSDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPV"
BASE COUNT         1101 a         1102 c         1178 g         1122 t
ORIGIN      
        1 atttacctaa aatacattcg ccacatgaat atatatttca atttacgagg ttttaattct
       61 gcctctttca acccgcgtca aaataaaaca gtagaatatt aatctttttt tgtgtttatg
      121 tgccttgaga cgccagtatt cataactatt ccttacatcg acgaatgata atttgtagga
      181 tagcaaactg tatttttctt tctgcgagtt aacgcgttgc ctttctgggt aaataacgcg
      241 cttttggttt tttgaggaat agtaatgacg aaacatgcca ggtttttcct cctgccctcc
      301 tttattctga tctccgcggc tttaatcgcc ggttgtaacg ataagggaga agagaaagct
      361 cacgtcggtg aaccgcaggt taccgttcat attgtgaaaa cggccccgtt agaagttaag
      421 actgaattac caggccgcac caatgcttac cgtatagccg aagttcgccc acaggttagc
      481 gggatcgtac tgaatcgtaa tttcactgaa ggcagcgatg tacaagcagg ccagtccctg
      541 taccagatcg atcccgcgac ctatcaggca aattatgaca gcgcgaaagg cgaactggcg
      601 aaaagtgaag ccgccgccgc catcgcgcat ttgacggtaa aacgttacgt tccgctcgtg
      661 ggtacgaaat acatcagcca gcaggagtac gaccaggcca ttgctgatgc tcgccaggcc
      721 gatgccgccg tgattgccgc aaaagccaca gtcgaaagcg ctcgcatcaa tctcgcttat
      781 accaaagtga ctgcgccaat tagcggacgt atcggcaaat cgaccgtgac cgaaggcgct
      841 cttgtcacta atgggcaaac gactgaactg gcgactgtcc agcagctcga tcctatctac
      901 gttgatgtga cccaatccag caacgatttt atgaggctga agcaatccgt agagcaagga
      961 aatttgcata aggaaaacgc caccagcaac gtagagttgg tcatggaaaa cggtcaaacc
     1021 tatcccctga aaggtacgct gcaattctct gatgtgaccg ttgatgaaag caccggctcc
     1081 ataaccctac gtgccgtctt ccctaacccg caacatacgc ttttgccggg tatgtttgtg
     1141 cgcgcacgga ttgatgaagg cgtccaacct gacgccattc ttatcccgca acaaggcgtt
     1201 agccgcacac cgcgtggtga tgcaaccgtg ctgattgtta acgataaaag tcaggtggaa
     1261 gcgcgccctg tcgttgccag tcaggcgatt ggcgataaat ggttgattag cgaaggtctg
     1321 aaatctggcg atcaagtcat tgtcagcggc ctgcaaaaag cgcgtccggg agagcaggtt
     1381 aaagccacta ccgatacccc cgcagatact gcatcgaagt aaggtaatct gacatggcaa
     1441 acttttttat tcgacgaccg atatttgcat gggtgctggc cattattctg atgatggcgg
     1501 gcgcactggc gatcctacaa ttacccgtcg ctcagtatcc aaccattgca ccgcctgcgg
     1561 tttctgtttc agcaaactat ccgggcgctg atgcgcagac cgtgcaggat acggtgacgc
     1621 aggttatcga acagaatatg aacggtatcg ataacctgat gtatatgtcc tccaccagcg
     1681 attccgccgg tagcgtgaca attaccctta ccttccagtc cgggaccgat cctgatatcg
     1741 cgcaagtgca ggtgcagaac aaactccagc tcgccacgcc gttgctgccg caggaggttc
     1801 agcagcaggg gatcagtgtt gaaaagtcca gtagcagcta tttgatggtg gcgggctttg
     1861 tctctgataa cccagacacc acacaggacg atatctcgga ctatgtggcc tctaacgtta
     1921 aagatacgct tagccgtctg aatggcgtcg gtgacgtaca gcttttcggc gcacagtatg
     1981 cgatgcgtat ctggctggac gccgatctgc taaacaaata taaactgaca ccggttgatg
     2041 tgattaacca gttgaaggta cagaacgatc agatcgctgc cggacagttg ggcggaacgc
     2101 cagcgttacc agggcaacag ttgaacgcct caattattgc tcagacgcgg ttaaaaaatc
     2161 cggaagaatt cggcaaagta accctgcgcg taaacagtga cggctcggtg gtacgcctga
     2221 aagatgtcgc acgagttgaa cttggcggtg aaaactataa cgttatcgct cgtatcaacg
     2281 gaaaaccggc ggcgggcctg gggattaagc tggcaaccgg cgcgaatgct ctcgataccg
     2341 cgaaagccat taaggcaaaa ctggcggaat tacagccatt cttcccgcag ggaatgaagg
     2401 ttctctaccc ttatgacacc acgccattcg tccagctttc tattcacgaa gtggtaaaaa
     2461 cgctgttcga agccattatg ctggtgttcc tggtgatgta tctgttcttg cagaatatgc
     2521 gagcaacgct gatccccacc attgcggtac ccgtggtgtt attagggacg tttgccatcc
     2581 tcgccgcttt tggttactcc atcaacacac taacgatgtt cgggatggtg cttgccatcg
     2641 ggctgctcgt cgatgatgcg atagtggtgg tggagaacgt cgagcgcgtg atgatggagg
     2701 ataagctccc gccaaaagaa gcgacggaaa aatcgatgtc gcaaattcag ggcgcactgg
     2761 tgggtatcgc gatggtgctg tcagcggtat ttattccgat ggcattcttc ggcggttcta
     2821 ctggggcaat ttatcgccag ttctctatca ccatcgtttc ggcaatggcg ctttctgttc
     2881 tggtggcatt gattcttacc cctgcgttat gtgcaacact gctcaaaccc acctcagctg
     2941 agcatcacga aaataagggt ggtttcttcg gctggtttaa taccaccttc gatcatagcg
     3001 ttaaccacta caccaacagc gtcggcaaaa tcctcggttc cacaggacga tatttactga
     3061 tctatgcgct gattgttgca ggaatggtgg tgttgttttt acgtcttccg tcttccttct
     3121 tacctgaaga ggatcagggt gtctttctga ccatgattca gttacccgct ggcgcgacgc
     3181 aagagcggac gcaaaaagtg ttggatcaag tgacggatta ctatctgaag aacgagaaag
     3241 cgaacgttga aagtgtcttt acggttaacg gctttagctt cagcggccaa gcacaaaacg
     3301 ccggtatggc cttcgtcagt ctgaaaccgt gggaagagcg taatagtgat gaaaacagtg
     3361 cggaagcggt aatccatcgt gccaaaatgg aattgggcaa gatccgcgac ggttttgtca
     3421 ttccattcaa tatgccagcc attgttgaac tgggcacggc aacgggtttc gactttgagt
     3481 taattgatca ggctgggctg ggtcacgatg ccctgaccca ggcccgtaac cagttgcttg
     3541 gcatggcggc gcaacatcct gccagcttag tcagcgtgcg tcctaatggc ctggaagaca
     3601 ccgcgcagtt taaactggaa gttgaccagg aaaaggcgca ggcattaggt gtttcacttt
     3661 ctgacatcaa tcagaccatt tcaacggcgt tgggtgggac ttacgttaac gacttcatcg
     3721 accgtggtcg cgtgaaaaag gtgtatgttc aggcggatgc caaattccgt atgctgccag
     3781 aagatgtcga taaactttat gtccgcagcg ccaacggcga aatggtgcca ttctcggcct
     3841 ttaccacttc acattgggtg tatggctctc cgcgactgga acgctacaac ggtctgccgt
     3901 caatggagat tcagggggaa gccgcgccag gaaccagttc cggcgatgcc atggcgttga
     3961 tggaaaacct tgcgtcaaaa ttacctgcgg gcattggtta tgactggacg ggtatgtcgt
     4021 atcaggaacg cttatcggga aaccaggctc ccgctctggt agcaatttcc tttgtggttg
     4081 ttttcctgtg ccttgctgca ctctatgaaa gctggtcaat tcctgtctcg gttatgttgg
     4141 tagtgccgtt agggattgtc ggcgtgctac tggcggcaac actctttaat caaaaaaatg
     4201 acgtctactt tatggtgggc ttgctaacga caattggctt gtcggccaaa aacgctattt
     4261 tgattgttga gttcgctaaa gatctcatgg agaaagaggg taaaggtgtt gttgaagcga
     4321 cactgatggc agtacgtatg cgtctgcgtc ctatcctgat gacctctctc gcctttattc
     4381 tcggcgtatt accgctagct atcagtaacg gtgccggcag tggcgcgcag aacgctgtgg
     4441 gtatcggggt aatgggagga atggtctctg caacgttgct ggcaatcttc ttcgtaccgg
     4501 tgt
//
LOCUS       DAZGAN020000218         4453 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGAN020000218 DAZGAN020000000
VERSION     DAZGAN020000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.219"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            6..81
                     /locus_tag="ACHKYO_RS24665"
                     /old_locus_tag="pgaptmp_004933"
     tRNA            6..81
                     /locus_tag="ACHKYO_RS24665"
                     /old_locus_tag="pgaptmp_004933"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHKYO_RS24670"
                     /old_locus_tag="pgaptmp_004934"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHKYO_RS24670"
                     /old_locus_tag="pgaptmp_004934"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /gene="yhdZ"
                     /locus_tag="ACHKYO_RS24675"
                     /old_locus_tag="pgaptmp_004935"
     CDS             complement(299..1057)
                     /gene="yhdZ"
                     /locus_tag="ACHKYO_RS24675"
                     /old_locus_tag="pgaptmp_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGU4431607.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIAVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /gene="yhdY"
                     /locus_tag="ACHKYO_RS24680"
                     /old_locus_tag="pgaptmp_004936"
     CDS             complement(1065..2168)
                     /gene="yhdY"
                     /locus_tag="ACHKYO_RS24680"
                     /old_locus_tag="pgaptmp_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU4431608.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /gene="yhdX"
                     /locus_tag="ACHKYO_RS24685"
                     /old_locus_tag="pgaptmp_004937"
     CDS             complement(2178..3359)
                     /gene="yhdX"
                     /locus_tag="ACHKYO_RS24685"
                     /old_locus_tag="pgaptmp_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU4431609.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWFFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3427..4452)
                     /locus_tag="ACHKYO_RS24690"
                     /old_locus_tag="pgaptmp_004938"
     CDS             complement(3427..4452)
                     /locus_tag="ACHKYO_RS24690"
                     /old_locus_tag="pgaptmp_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4431610.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1206 a         1180 c         1072 g          995 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgccgg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctaccgc gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaacctgaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggtgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatactgcc ctttaggcag tgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg acacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagcc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccagttag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagaa ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga tagttttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcctaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggcg
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgccc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggca acgttgagtt cggtatcagt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 ataagtgacg ccggtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cttcccctga ctgtaaagcg gtgaagcgtt cttttgctgt
     4201 gagtggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatcaaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctga acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ata
//
LOCUS       DAZGAN020000219         4232 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGAN020000219 DAZGAN020000000
VERSION     DAZGAN020000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.220"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..138
                     /locus_tag="ACHKYO_RS24695"
                     /old_locus_tag="pgaptmp_004939"
     CDS             <1..138
                     /locus_tag="ACHKYO_RS24695"
                     /old_locus_tag="pgaptmp_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431611.1"
                     /translation="SVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            215..898
                     /locus_tag="ACHKYO_RS24700"
                     /old_locus_tag="pgaptmp_004940"
     CDS             215..898
                     /locus_tag="ACHKYO_RS24700"
                     /old_locus_tag="pgaptmp_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HGU4431612.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRFGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            899..1120
                     /locus_tag="ACHKYO_RS24705"
                     /old_locus_tag="pgaptmp_004941"
     CDS             899..1120
                     /locus_tag="ACHKYO_RS24705"
                     /old_locus_tag="pgaptmp_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431613.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            1101..1568
                     /locus_tag="ACHKYO_RS24710"
                     /old_locus_tag="pgaptmp_004942"
     CDS             1101..1568
                     /locus_tag="ACHKYO_RS24710"
                     /old_locus_tag="pgaptmp_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU4431614.1"
                     /translation="MRASKIEKETRMYGTCETLCRALAAKYPGDTPLMLVIWSPEEIQ
                     ALADGMDIALTDHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVRENRQVTVPAE
                     LLASLIQTAEQALWKREWAARDHGLAVPECVTRRQAVVNQVRILLKNNTHEND"
     gene            1613..2383
                     /locus_tag="ACHKYO_RS24715"
                     /old_locus_tag="pgaptmp_004943"
     CDS             1613..2383
                     /locus_tag="ACHKYO_RS24715"
                     /old_locus_tag="pgaptmp_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431615.1"
                     /translation="MNETLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRK"
     gene            2799..3224
                     /locus_tag="ACHKYO_RS24720"
                     /old_locus_tag="pgaptmp_004944"
     CDS             2799..3224
                     /locus_tag="ACHKYO_RS24720"
                     /old_locus_tag="pgaptmp_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4431616.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            3271..3690
                     /locus_tag="ACHKYO_RS24725"
                     /old_locus_tag="pgaptmp_004945"
     CDS             3271..3690
                     /locus_tag="ACHKYO_RS24725"
                     /old_locus_tag="pgaptmp_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU4431617.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIARDWNLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            3690..3881
                     /locus_tag="ACHKYO_RS24730"
                     /old_locus_tag="pgaptmp_004946"
                     /pseudo
     CDS             3690..3881
                     /locus_tag="ACHKYO_RS24730"
                     /old_locus_tag="pgaptmp_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3896..4198
                     /locus_tag="ACHKYO_RS24735"
                     /old_locus_tag="pgaptmp_004947"
     CDS             3896..4198
                     /locus_tag="ACHKYO_RS24735"
                     /old_locus_tag="pgaptmp_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431618.1"
                     /translation="MEVACQHARKPLAGWRLPEGMRATLARRPLPFLLVTGVVSGSTC
                     YRPVVKEPSYTCAPSAPGCLAASGRQARLRQAAAGAPASLRRTRWRGFGLPLRSDA"
BASE COUNT          877 a         1171 c         1290 g          894 t
ORIGIN      
        1 tcggtgacag tgtacgccag cccgtcgccc ttttctgatg aacgcccttc aagccgcttt
       61 cgcggcatag ccttgccgtc aaaaagacga cggctgcggt attccacggt cggcctgacc
      121 cgttaccgga cgcggtgaac agcccacagg cagcggggaa cgggcaccac aggggtgccc
      181 tcctggtgcc ttctgtaaga gaaggagtgt tgttatgtcc cgttttgtcc tcggtaactg
      241 catcgatgtt atggcccgta ttcctgataa cgccattgat ttcatactga ctgacccgcc
      301 gtacctcgtc ggttttcgtg accgtttcgg gcgcaccatc gctggcgata aaaccgatga
      361 atggctgcaa ccggcctgta atgaaatgta ccgcgtactc aaaaaagacg cgctgatggt
      421 cagcttctac ggctggcacc gtattgaccg ctttatggcg gcctggaaac gcgccggatt
      481 cagcgtggca gggcacctcg tgttcaccaa aaactacaca tccaaatctg cgtatgtcgc
      541 gtaccgccac gaatgcgctt acatcctggc aaagggcagt ccggcccggc cccgtaaccc
      601 tctgccggat gtgctgggct ggaaatattc aggcaatcgt catcacccga cggaaaagcc
      661 cgttaccagc ctgcaaccac tgattgagag cttcacacat ccgaacgcga ttgtgctgga
      721 cccgtttgca ggcagcggct caacctgcgt ggccgccctt cagtccggac gccggtatat
      781 cggtatcgag ctgcttgaac agtatcaccg tgccggacag caacgactgg ccgccgtgca
      841 acgggccatg cagcaggggg ccgcgaatga tgactggttt atgccggagg ctgcgtaaat
      901 gaactatgcc ggacacgaaa aactgcgcgc cgaagtggcg gaggtggcca atgccatgtg
      961 cgacctgcgt acaaccatga atgagatgga gcagcggtac agctttaatg ccgacaccct
     1021 gccggaacgt ctggtgcgtc agacgctgtt tcgcgcaaac cgcctcctga tggaggcata
     1081 taccgaaatt ctggaactgg atgcgtgctt caaagattga gaaggagacg agaatgtacg
     1141 gaacatgcga aacgctctgc cgggcgctgg cagcaaagta tccgggagat acgccgctga
     1201 tgctggttat ctggtccccg gaagaaattc aggccctcgc tgacggaatg gatattgccc
     1261 tgaccgatca tgaaatcagg actgtcctgg cgcgcctgga ggacatcccg gaagaccagc
     1321 ggactgaatc cggtatttct tccggcgtgg cgatggagat catcaataac gtgagagaaa
     1381 accgccaggt gaccgttccg gctgaactgc tggcgtccct gattcagacc gctgaacagg
     1441 cattgtggaa acgtgaatgg gccgcccggg atcatggcct cgccgtcccg gaatgcgtca
     1501 cccgccgtca ggcggtggtt aatcaggtcc gcatcctgct gaaaaacaac acacacgaaa
     1561 acgactgatg ttatcgccgc ctccgggcgg cgatacaggg agttcatgaa ttatgaatga
     1621 aacgctgaat gcactgattt atcgccacgc cagcaacctg ttgctggcgc agggctggcc
     1681 ggaagaaacg gatgttgacc agcgtaaccc gaagtatccg ggctggatca gcatttacgt
     1741 gcggctggat gcgccccggc tggcgacgtt acttatcaac cgtcacggcg gcgtactgcc
     1801 gccgctcctg gcctctgcca ttcagaaact gaccggaacc ggggcggaac ttgttctgtc
     1861 cggcagtcag tggcagtcgc tgccggtact tccggcagac ggaacgcagg tgtctttccc
     1921 gtatgccgga gaatggctga ccgaagacga aatcagggcc gttcttgatg cggtgcacga
     1981 tgcggtacgc agtgtcagtt gccgggtggc agaagatgcg cggcgtatcc gggcggcgct
     2041 taccacaacc ggtcagacgt tgctgacccg ccagacgcgc cgctttcgcc tggttgtgaa
     2101 ggaaagcgat cacccctgct ggctcgatga agacgacgaa aacctgcccg tggtgctcga
     2161 tgccattctg aaccggggag cacgtttttc ctctgtggaa atgtacctgg tcagtgagtg
     2221 tgtggagcat atcctggcca gtggcctggt ctgtgatgtg ctgcgtatac cggatgaacc
     2281 gtcccgtcgc tggtttgaca gggatattct gcgggaggtt gttctggagg cacgggatga
     2341 aatccgcagc atggcggatg ccctggcaaa aatccggaaa tgatgacgct ggcggggaaa
     2401 aatctccgcc ttttcctgcc ggtccccgga cagagcacaa aggatgtcgc cgccggctcc
     2461 gctttacccg gccatgcggg gcatggcctt gtgggttttc agttatgtgg cctcagcgtc
     2521 gtgtgcgggc tgtgccgtgc ctccatcgta gccgggcttc tgcgggcggg ttgcaaggta
     2581 aggtgaaccg ctgcggtcac ccggtccctc cttcccgtct gccgtgattt tccggtcgtt
     2641 cacccgctca ggtttcgcgg tctcaccctc caactcccgt cagccgtgtg cgtcaggctg
     2701 cggcttccct tgcgcccttg caaccctgca ggcccggcaa agatggaggc acggcaccgc
     2761 ccggtgtcgc ggaatatgtg aacaacggag gaaatcccat gcagtatgcg aaacctgtca
     2821 ccctgaacgt tgaagagtgc gaccgccttt cttttctgcc ttacctgttt ggcaacgatt
     2881 ttctgtatgc cgaggcgtat gtgtacgcgc tggcgcaaaa aatgatgccg gaatatcagg
     2941 gaggcttctg gcatttcatc cgcctgccgg acggtggcgg ttacatgatg ccggatggcg
     3001 accgttttca tctggtcaac ggtgaaaact ggtttgaccg cactgtgagt gctgatgccg
     3061 caggtatcat cctgacttcc cttgtgatta accgccagtt gtggctgtac cacgacagcg
     3121 gtgatgcggg actgacacac ctgtaccgga tgcgcgatgc gcagttgtgg agccacattg
     3181 aatttcaccc cgaatgtaac gcgatttatg cagcactgga ctgattaacg gacggggcgg
     3241 cgatgccgcc cctgtaaagg agacgagaac atgtactgta ctgttaaaga aattatccgc
     3301 gaagtgctga atacagacgt gccggacagt gaatgtgttt ttgccgtggt gctgacccgc
     3361 ggggatgtgc gccacatagc ccgggactgg aacctgtcag acgatgagct ggaaaccgtc
     3421 atgcagcggc tggacgatgc ttttgtatat ggcgcatgtg acagagttgt ttccgatatt
     3481 gtcaatgaac tgatggagga aaagcgcgta aaccgtcttg taacagttcc tgcggtgctg
     3541 ctggaaaaag tgatggtgat ggctggtagt gaaatttatc gcctccatgc tgtcgggagc
     3601 gagaacgggg gcgacggtga cgcatttgtc agggaagaac gtgagattat gcgggtaatg
     3661 cgtcaggcgc tggacgggga gaacgggtaa tgaatatcag cacggaaacc cgcgaaattc
     3721 tgcgtaatta aaaagctgtg attaatgcac gacgtcgtga aatggggcag aaaccgctca
     3781 ccaccgcgca gattgttgat gaaatctgcg attttgtggt gaatcagcag gctgttttcc
     3841 tgggtggtca ctatatcctt cagggcagca ggcacaggtg atacaggggc aggcggtgga
     3901 ggtcgcctgt cagcacgcgc gaaaacccct ggcaggctgg cgcctgccgg aagggatgcg
     3961 ggccacgctg gcgcgtcgcc cgttaccctt cctgttggtg acaggcgttg tttcaggaag
     4021 tacctgttac agacctgtcg ttaaagaacc ttcttacaca tgcgcccctt cggctcccgg
     4081 ctgcctggcg gcatccggtc gtcaggcccg actccgacag gcagctgctg gcgcacccgc
     4141 cagtctccgc cgaacccgct ggcgcggttt cgggctgccg ctacggtccg atgcgtgaaa
     4201 acccgggctt gcagcgaccg ttccggtcgc gc
//
LOCUS       DAZGAN020000220         4179 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGAN020000220 DAZGAN020000000
VERSION     DAZGAN020000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.221"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            8..109
                     /locus_tag="ACHKYO_RS24740"
                     /old_locus_tag="pgaptmp_004948"
                     /pseudo
     CDS             8..109
                     /locus_tag="ACHKYO_RS24740"
                     /old_locus_tag="pgaptmp_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            334..825
                     /gene="ygeG"
                     /locus_tag="ACHKYO_RS24745"
                     /old_locus_tag="pgaptmp_004949"
     CDS             334..825
                     /gene="ygeG"
                     /locus_tag="ACHKYO_RS24745"
                     /old_locus_tag="pgaptmp_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4431619.1"
                     /translation="MSTETIEIFNNSDEWANQLKHLLSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNVTDAEIYYKFLCIYAFENHEYLEGFASVCQSKKKYQQAYDLYKLSYN
                     YFPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            1159..2535
                     /gene="ygeH"
                     /locus_tag="ACHKYO_RS24750"
                     /old_locus_tag="pgaptmp_004950"
     CDS             1159..2535
                     /gene="ygeH"
                     /locus_tag="ACHKYO_RS24750"
                     /old_locus_tag="pgaptmp_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HGU4431620.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTPESIYR
                     AMTIFDRLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLK
                     QLTRICMR"
     gene            2703..2921
                     /gene="ygeI"
                     /locus_tag="ACHKYO_RS24755"
                     /old_locus_tag="pgaptmp_004951"
     CDS             2703..2921
                     /gene="ygeI"
                     /locus_tag="ACHKYO_RS24755"
                     /old_locus_tag="pgaptmp_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HGU4431621.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
     gene            3004..3506
                     /locus_tag="ACHKYO_RS24760"
                     /old_locus_tag="pgaptmp_004952"
                     /pseudo
     CDS             3004..3506
                     /locus_tag="ACHKYO_RS24760"
                     /old_locus_tag="pgaptmp_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(3525..4157)
                     /locus_tag="ACHKYO_RS24765"
                     /old_locus_tag="pgaptmp_004953"
                     /pseudo
     CDS             complement(3525..4157)
                     /locus_tag="ACHKYO_RS24765"
                     /old_locus_tag="pgaptmp_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
BASE COUNT         1522 a          712 c          663 g         1282 t
ORIGIN      
        1 ataataaaga aaaaaaacaa acgacattct acccttcgca aaaatcccct ttttaatttt
       61 cattgaaaaa atggcaattg atactatgaa tccaggtcat acacattaag gcatttatgt
      121 aatgtcgtaa ttaagacaac taataaggtg aatattagta aaacaactaa tgatgttatt
      181 attttgtagt gaacagtact tttaccaata atgaaaaata taccataagc aattcaagct
      241 taaaaccaat aatttaaaaa agataaatgt aaaaatatta atgagtcaat caataataat
      301 ttagcatcac gagacacatc acagaggaat attatgagca cagaaacaat tgaaatattc
      361 aataatagtg atgaatgggc aaatcaacta aaacacctat tatcgaaagg agaaaatctg
      421 gcattactac atggtttaac tcctgatatc cttgatagaa tatatgcata tgcattcgac
      481 taccatgaaa agggtaatgt aacggacgca gaaatttatt ataaattttt gtgcatttat
      541 gcgttcgaaa atcatgagta tctagaaggt tttgcatcag tatgtcagtc aaaaaagaaa
      601 tatcaacaag catatgacct ttacaaacta agttacaatt acttcccgta tgatgactat
      661 tcagttattt atcgtatggg tcaatgtcag attggggcta aaaatatcga taacgcaatg
      721 caatgtttct atcacattat taacaattgt gaggatgata gtgttaagag taaagcgcag
      781 gcatatattg aactcttaaa cgataattca gaagataatg gctaagcatt acaatcattt
      841 ggttaaattt gctggttata gtgcagttaa ccactataac cagcacctct tatgttttct
      901 taaatcagca tcttaaaaca gttaagagca tactataaat cattattttt caatgcgtta
      961 gagaacaatc tactttatca aaattgaata aggctatttc agattacatc actgaattcc
     1021 taagataaat tagtgaacac aaccatagaa tgtcgaatgt aatttcctgt agcgttaatg
     1081 acacataaat atgaatagcc ataatttcta ttgcttaagc aggatgcaag aaaccaattt
     1141 tttcatagag gttaactaat ggacttagaa aataaattct catatcattt tcttgaggga
     1201 ttaacgctca cggaagatgg aattcttact caaggaaatg agcaagttta tattccacag
     1261 aaagagttag gtgtattaat agtattactt gaatccgctg gtcatgttgt actgaaagat
     1321 atgatcatcg aatcagtatg gaaaaatatt attgttagtg acgagtccct gacaagatgt
     1381 atctattctt tgcgctgcat ttttgaaaaa attggctatg atcgttgcat agaaacaatc
     1441 taccggaaag gttatcgttt cagcgggcag gttttcaaaa ctaaaattaa tgaagataat
     1501 acttcagact attccatagc tatattccct ttcactactt cgttgaatac actggatcca
     1561 ttaatactta atcaggaatt agtgcaaatc atttcaaata aaaaaatcga tggtctctat
     1621 acctatccga tggctgcgac aaatttttgt aatgatcaca tatctcaaaa ttcattcttg
     1681 agcagattca agccagatta tttcgttaca ggaagaataa accagaataa tgcagtgaac
     1741 actttataca ttgagttgat cgacgctaaa aaccttttcc tcatcgccag taatcatctc
     1801 cctgttgatg aactacataa tacatcacaa tttattatag ataatatcct tcaaacggta
     1861 cataaaccag aacgatctgt aagattagct aagcaggacc aaggatataa gaatcattat
     1921 ttatcagatg aaatgttagc cggaaagaaa gaactttacg acttcacccc tgaaagcatt
     1981 tacagggcca tgactatatt tgatagatta caaaataaaa gtgatataca gacgctaaaa
     2041 acagaatgtt attgccttct agcggaatgc catatgtctt tggcacttca tggaaaaagt
     2101 gaacttgaac ttgctgctca aaaagcatta gagcttttag attatgtatc agacataacc
     2161 actgtcgatg gaaaaatttt agctattatg ggactgataa ctggtctgtc tggacaagca
     2221 aaagtatctc atatcttatt tgaacaggct aagatacact caactgatat agcctctctc
     2281 tactactata gggcactagt ccactttcat aatgaaaaaa ttgaagaggc aaggatttgt
     2341 atagacaaat cactacaact cgaacccaga agacgaaaag cagttgtgat aaaagaatgt
     2401 gtagatatgt atgtgcctaa cccgctcaaa aacaatatta aactctacta taaagaaact
     2461 gagagtgaaa gccatcgagt tataattgac aacattttga aattaaagca gctgacgaga
     2521 atttgtatgc gataaatttt tattgacgca attttattat ttagatgtat gcaacttgtt
     2581 atttaaacaa cagagataaa aatatctaag atattcacct tattgcaaga tatttaaaat
     2641 gctctagagt taaaagtatg atgtaaagac cattggcctg gacaattaaa aaagggagct
     2701 ttatgactaa tccaatcggt atcaataatt tatctcaaag ttcaaatata gcgaatgcaa
     2761 cgggtgatga agtcgtatca ttagataaac acatcaacac cagtgctact gatacagatc
     2821 aaatacaagc atttatagtt tcaacatgga tggcgccttt tcaaaatgat atgtattcag
     2881 aagataatcc tatctcacct tactataaaa ttgaatggta gacgattgac caaataaatc
     2941 ataacgagat acttttataa tagtatctcg tccctttcat tcaatcctca cacatgaaaa
     3001 aatatgcgct caccaaaagt taaattctta accatcttca cgttttgtat atttattaca
     3061 aaaatgtctt tcgccagcaa ctcttgctct aatgaagcag gaacaatgtt cagaatcgaa
     3121 ccaaatttaa ttaaagctat cgctcttgtt gaaagcaatc taaaaaagga tagcataggc
     3181 aagaacagag ataagaataa caatataaag agtttagatt attggttaat gcagataaac
     3241 cagatgcata ttcccctgtt aaaaaaacgt ggaataataa aagatgaacg agatttgtta
     3301 gataaccctt gtctgaacat taaaatagga accgagattt tatataacca tttttcacgt
     3361 tgtggagtaa cgtggcaatg cctggggact tataatgcgg gttttgcaat ggataaccaa
     3421 aaaaagagac agcaatatgc cccaaaatat atattgtata taccaggctt aatgaactag
     3481 acaatcgtaa ggcttcagca aaatgagcct aaagcctctt ttttttatat agtgcaaaca
     3541 cccatacgta aagctgtttt gatcaactcg ataccactgt gaacatctaa tttcttcatg
     3601 atattgagtc tatgagtttc gactgttttt ctgcttaact gcaagaaatt agcgatttct
     3661 ttgtttgttt taccatcagc tatgagttgt aatatttctg actcacgatt tgtaagttga
     3721 ttatcagagg aatacctgga agatattttt tcacaatcca tatgtacact atcaaaataa
     3781 gtgtaaccat tactaatcga atcaatagca cgatttagtt tcgccgggtg actggttttc
     3841 catacgcatc ctttcgcacc tgcttcaagc aattttattg aatcaatata cggcatcttt
     3901 gctacaaatg taataatatg agcatctatc ttatgcgatt taacccattt tacgagttcg
     3961 gaccccatca ttccacctgc aaattctcct aaaataaata tttaggctgt agatttgtta
     4021 cactccgcta tagctttctt cagatcttta tagcccccaa caaccttata taaatcagga
     4081 taatgtccga gaaatccaat tatcccatca atcataaacg gctgctgatc tgaaactaca
     4141 attttaattt ttcccatata ttcatcctga gtgtaaatg
//
LOCUS       DAZGAN020000221         4147 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGAN020000221 DAZGAN020000000
VERSION     DAZGAN020000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.222"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            144..752
                     /locus_tag="ACHKYO_RS24770"
                     /old_locus_tag="pgaptmp_004954"
     CDS             144..752
                     /locus_tag="ACHKYO_RS24770"
                     /old_locus_tag="pgaptmp_004954"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HGU4431622.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            850..2241
                     /gene="selA"
                     /locus_tag="ACHKYO_RS24775"
                     /old_locus_tag="pgaptmp_004955"
     CDS             850..2241
                     /gene="selA"
                     /locus_tag="ACHKYO_RS24775"
                     /old_locus_tag="pgaptmp_004955"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HGU4431623.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2238..4082
                     /gene="selB"
                     /locus_tag="ACHKYO_RS24780"
                     /old_locus_tag="pgaptmp_004956"
     CDS             2238..4082
                     /gene="selB"
                     /locus_tag="ACHKYO_RS24780"
                     /old_locus_tag="pgaptmp_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HGU4431624.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          948 a         1036 c         1214 g          949 t
ORIGIN      
        1 atcagataga aacaattagg aaaatattta tatcaccatg ataaatatgt gatttatttg
       61 gaagtaacaa accatcaaca ttttctgccc tttttcccat caccaggcca tactgatatt
      121 tttcctcgca caaaggactc ggcatgaaac tcgtcggtag ctacaccagc ccgtttgtac
      181 gcaaactttc tattctgttg ttagaaaagg gcataacttt cgaatttatt aatgaactgc
      241 cctataacgc ggacaacggc gtggcgcaat ttaacccgtt aggaaaagtg ccggtgctgg
      301 tgaccgaaga gggcgaatgt tggtttgatt cgccgatcat cgctgaatat attgaattaa
      361 tgaatgtcgc tccggcgatg ttgccgcgcg atccgctgga gtcgttgcgg gtgcgcaaaa
      421 ttgaggcact ggcggatggc attatggatg ccgggctggt atcggtgcgt gaacaggcgc
      481 gtccagcggc gcagcagtct gaagatgaat tgttacgcca gcgggagaaa atcaaccgca
      541 gtctggatgt gctggaagga tatctggtcg atggcacact caaaaccgat acggtcaatc
      601 tggcgactat cgccattgcc tgtgccgtcg gatatctcaa tttccgccgc gttgcgccag
      661 gctggtgtgt cgatcgccca cacttagtca aactggtcga aaacctgttt agccgcgaaa
      721 gttttgctcg caccgaaccg ccaaaggctt gatgcgcgat atgtcctcct gacccatctc
      781 acgttacaat ccgtggttat gttaaacgcc cttctccgtg tgagagggcc ttgatcagcc
      841 aggtttccta tgacaaccga aacgcgttcc ctctatagtc aacttccggc tattgatcgc
      901 ttattgcgcg atagctcctt cctttctttg cgtgatactt atggtcacac ccgcgtggtg
      961 gaattgttgc gtcagatgct cgacgaagcg cgagaagtga ttcgtggcag ccagacgctg
     1021 cctgcgtggt gtgaaaactg ggcgcaagaa gtcgatgccc ggttgacgaa agaagcgcag
     1081 agcgcgctgc gtccggtgat caacctgacg ggaaccgtgc tgcataccaa ccttgggcga
     1141 gctttacagg cggaagccgc ggtggaagcc gttgcgcagg ctatgcgttc gccagtgacc
     1201 ctcgagtacg atctggacga cgctggacgc ggacatcgcg atcgtgcgct ggcgcagttg
     1261 ctgtgccgca ttacgggggc ggaagatgcc tgtatcgtta ataacaatgc ggcggcggtg
     1321 ttattgatgt tggcggccac tgccagcggg aaagaggtgg tggtttctcg cggcgaactg
     1381 gtggagattg gcggcgcgtt tcgtattccc gatgttatgc gtcaggcagg ctgcacccta
     1441 cacgaagtag ggaccaccaa ccgcacgcac gcgaatgatt atcgtcaggc ggtgaatgaa
     1501 aataccgcac tgttgatgaa agtacatacc agtaactaca gcattcaggg gttcaccaaa
     1561 gcgatagatg aagcggaact ggtggcgctc ggcaaagagc tggatgttcc cgtagtgact
     1621 gatttaggca gtggctcgct ggtcgatctt agccagtacg gtttgccgaa agagccaatg
     1681 ccgcaggagt tgattgcggc gggcgtcagt ctggtgagtt tctccggcga caagttgtta
     1741 ggcgggccgc aggcaggaat tattgttggt aaaaaagaga tgatcgcccg cctgcaaagc
     1801 cacccgctga agcgtgcatt acgcgcggat aaaatgaccc tcgcggcgct ggaagccacg
     1861 ttgcgtcttt atttacaccc tgaagctctg agtgaaaaat taccgaccct gcgcctgctt
     1921 acccgcagcg cagaggtcat tcaaatccag gcacaacgtt tacaggcccc ccttgccgca
     1981 cattacggcg cggagtttgc ggtacaggtt atgccatgtc tttcgcagat tggcagtggt
     2041 tcgctgccgg ttgatcgcct gccgagcgcg gcattaacgt ttacacccca tgatggacgc
     2101 ggtagccacc ttgagtcatt agccgcccgc tggcgtgaat tgccagtgcc ggtgattggt
     2161 cgtatttatg acggacgatt gtggctggat ttacgctgcc ttgaagatga gcaacggttt
     2221 ttggagatgt tgttgaaatg attattgcga ctgccggaca cgttgaccac ggcaaaacaa
     2281 ccttattgca ggcgattact ggcgtaaatg ctgaccgtct gccggaagaa aaaaagcgcg
     2341 gcatgaccat cgatctcggc tatgcctact ggccgcagcc ggatggtcgc gtgcctggtt
     2401 ttatcgacgt acccggtcat gaaaagtttc tttccaacat gctggcgggc gttggtggta
     2461 tcgatcacgc gctgttggtg gtggcgtgcg atgacggcgt gatggcacag acccgtgagc
     2521 atctggcgat tttgcagctg accggtaacc cgatgctgac agtggcgctg accaaagccg
     2581 atcgcgtgga cgaagcgcgt gttaatgagg ttgaacgcca ggtaaaggag gtgctgcggg
     2641 aatacggttt tgctgaggca aaactgttta tcaccgcagc aaccgaaggt cggggaattg
     2701 atgccctgcg cgagcatctg cttcagttgc cggaacgcga gcacgccagc caacatagtt
     2761 tccgcctcgc tattgaccgc gcatttaccg taaaaggtgc cgggctggtc gtcaccggta
     2821 cggcgttaag cggggaagtg aaggtaggcg attcactctg gctgactggt gtaaataaac
     2881 cgatgcgtgt acgtgcgctg catgcgcaaa accagccaac agaaaccgcc aatgccgggc
     2941 agcgtatcgc gcttaacatc gcgggtgatg cggaaaaaga gcagattaac cgtggcgact
     3001 ggctgcttgc cgatgtgccg ccagagccgt tcacacgggt gattgtcgag cttcaaaccc
     3061 atacaccgct gacccagtgg cagccgctgc atattcacca cgccgccagc cacgttacgg
     3121 gacgcgtttc gctgctggaa gataaccttg ccgagctggt gttcgacacg ccgctgtggc
     3181 tggcggataa cgatcgctta gtgctgcgcg atatctcggc ccgcaacacg ctggcggggg
     3241 cgcgtgtggt gatgcttaac ccgccgcgtc gcggtaaacg taagccggaa tatctgcaat
     3301 ggctggcgtc tcttgcacgg gcgcagagcg atgccgatgc gttatctgtt catctggaac
     3361 gcggcgcggt taaccttgcg gatttcgcct gggcgcgcca gctcaacggc gaagggatgc
     3421 gcgaattgct gcaacagcct ggctatattc aggctggtta tagcttgttg aatgcgccgg
     3481 ttgccgctcg ctggcaacgg aaaattctcg acacattagc gacttatcat gagcaacatc
     3541 gcgatgaacc tgggcctggg cgcgaacgtc tgcgacgtat ggcgttgcca atggaagatg
     3601 aagcgctagt actgttgctg attgaaaaga tgcgcgaaag cggcgacatc ctcagtcatc
     3661 acggttggct gcatctgccg gatcacaaag cgggcttcag cgaagagcag caggccatct
     3721 ggcaaaaagc agagccgctg tttggtgacg agccgtggtg ggtgcgtgat ctggcaaaag
     3781 agacgggtac cgacgagcag gcaatgcgcc tgacgctacg ccaggcggcg cagcaaggaa
     3841 taattaccgc gatcgtcaaa gatcgttatt accgtaacga tcggattgtc gaatttgcca
     3901 atatgatccg cgatctcgat caggagtgtg gttcaacctg cgcggcggat ttccgcgatc
     3961 gcttaggcgt aggccgaaag ctggcaattc agattctgga atattttgac cgcattggct
     4021 ttacgcgtcg tcgtggaaat gatcatttat tgcgcgacgc attattattt ccggaaaaat
     4081 aaggaaatga ttaattaatt tttaaaataa attaatacaa atttcttatg attttaaaaa
     4141 aagcaca
//
LOCUS       DAZGAN020000222         4122 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGAN020000222 DAZGAN020000000
VERSION     DAZGAN020000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.223"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..3086
                     /gene="evgS"
                     /locus_tag="ACHKYO_RS24785"
                     /old_locus_tag="pgaptmp_004957"
     CDS             <1..3086
                     /gene="evgS"
                     /locus_tag="ACHKYO_RS24785"
                     /old_locus_tag="pgaptmp_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HGU4431625.1"
                     /translation="ANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNI
                     ITSSMISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVS
                     QNWLDTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGD
                     ILNIITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITT
                     PYVFVMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKE
                     GELDALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALN
                     AIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVR
                     RRKVIQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLP
                     LENSDSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDN
                     AVYICGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLEL
                     LSGSGLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQN
                     TCHSFGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKIT
                     TSLVHIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKEL
                     IKNMQGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHP
                     TNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLRE
                     QNSSLPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYR
                     HLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGA
                     ANILNLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(3142..>4122)
                     /gene="yfdE"
                     /locus_tag="ACHKYO_RS24790"
                     /old_locus_tag="pgaptmp_004958"
     CDS             complement(3142..>4122)
                     /gene="yfdE"
                     /locus_tag="ACHKYO_RS24790"
                     /old_locus_tag="pgaptmp_004958"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HGU4431626.1"
                     /translation="YVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAE
                     NFRPGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMET
                     GYPDAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHG
                     LMAYIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVN
                     DPRFSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQ
                     TQARNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
BASE COUNT         1250 a          911 c          820 g         1141 t
ORIGIN      
        1 tagcaaatta ccccccagac gaggtaattc atcaatcatt tccaaaagca acaattatct
       61 cttttacaaa tttatatcag gcattagcat ccgtctcagc tgggcacaat gattacttta
      121 ttggtagtaa catcattacc agcagtatga tttcccgcta tttcactcac tccttaaatg
      181 tagtgaaata ttataactcg ccgcgtcaat ataatttttt cttgaccaga aaagaatctg
      241 tcattcttaa tgaagtactc aatagatttg ttgatgcttt aacaaatgaa gttcgctatg
      301 aagtatcaca aaattggctt gatacaggaa acctggcctt tctgaacaaa ccattagaac
      361 tcactgaaca tgaaaaacag tggattaagc agcatcccaa tttaaaggtg ctggaaaatc
      421 cttactcgcc cccctattct atgacggatg aaaatggctc ggttcggggc gttatggggg
      481 acattcttaa tattattacc ttgcaaacag gtttaaattt ttctccgatc accgtttcac
      541 acaatatcca tgctggaaca cagcttagcc ccggaggatg ggatataata cctggcgcta
      601 tttatagtga agatcgagaa aataatgttt tatttgctga agccttcata acaacgcctt
      661 acgtttttgt catgcaaaaa gcgcctgaca gtgaacaaac attaaaaaaa ggaatgaaag
      721 ttgccattcc atattattat gagctgcatt cgcaattaaa agagatgtat ccggaggttg
      781 aatggataca ggtcgataat gccagcgctg catttcacaa ggttaaggaa ggtgaacttg
      841 atgctctggt cgcgacacag ttaaattcgc gttacatgat cgatcattac tatcctaatg
      901 aactttatca ttttcttatt cctggcgttc cgaatgcatc gctttcgttc gcttttcctc
      961 gcggagaacc ggaacttaag gatattatta ataaagcact gaatgcaatt cccccaagcg
     1021 aagttctgcg cctgacggaa aaatggatta aaatgcccaa tgtgaccatt gacacatggg
     1081 acctatatag cgagcaattt tatattgtta cgacattatc cgttttatta gttggcagta
     1141 gccttttatg gggattctac ctgttacgct cagttcgtcg tcgtaaagtc attcagggtg
     1201 atttagaaaa ccaaatatca ttccgaaaag cactctcgga ttccttaccg aatccaactt
     1261 atgttgtaaa ctggcaaggt aatgtcatta gtcataatag tgcttttgaa cattatttca
     1321 ctgcggatta ctacaaaaat gcaatgttac cattagaaaa cagtgactca ccctttaaag
     1381 atgttttttc taatgcgcat gaagtcacag cagaaacgaa agaaaatcga acaatataca
     1441 cacaggtatt tgaaattgat aatggcatcg agaaaagatg cattaatcac tggcatacat
     1501 tatgcaatct tcctgcaagt gacaatgcag tatatatttg tggttggcaa gatattactg
     1561 aaacgcgtga tctaattaat gcactcgagg tagaaaaaaa taaagcgata aaggctaccg
     1621 tagcaaaaag tcagtttctg gcaacgatga gtcacgaaat aagaacacca ataagctcta
     1681 ttatgggctt cctggaactt ctgtcgggtt ctggtcttag caaggagcaa cgggtggagg
     1741 cgatttcact tgcctacgcc accggacaat cactcctcgg cttaattggt gaaatccttg
     1801 atgtcgacaa aattgaatcg ggtaactatc aacttcaacc acaatgggtc gatatcccta
     1861 ctttagtcca gaacacttgt cactctttcg gtgcgattgc tgcaagcaaa tccatcgcat
     1921 taagttgcag cagtacattt cctgatcatt acctggttaa gatcgatcct caggcgttta
     1981 agcaggtctt atcaaattta ctgagcaatg ctctcaaatt taccaccgag ggggcagtaa
     2041 aaattacgac ctccctggtt cacattgatg acaaccacgc tgttatcaaa atgacgatta
     2101 tggattctgg aagtggatta tcgcaggaag aacaacaaca actctttaaa cgctacagcc
     2161 aaacaagtgc aggtcgccag caaacaggat ctggcttagg cttaatgatc tgcaaagaat
     2221 taattaaaaa tatgcagggc gatttgtcat tagaaagtca tccaggcata ggaacaacat
     2281 ttacgatcac aatcccggta gaaattagcc agcaagtggc gactgtcgag gcaaaagcag
     2341 aacaccccat cacactacct gaaaagttga gcatattaat cgcggatgat catccgacca
     2401 acaggctatt actcaaacgc cagctaaatc tattaggata tgatgttgat gaagccactg
     2461 atggtgtgca agcgctacac aaagtcagta tgcaacatta tgatctgctt attactgacg
     2521 ttaatatgcc gaatatggat ggttttgagt tgactcgcaa actccgtgag caaaattctt
     2581 ccttacccat ctgggggctt acagccaacg cacaggctaa cgaacgtgaa aaagggttaa
     2641 gttgcggcat gaacttatgt ttgttcaaac cgttgaccct ggatgtactg aaaacacatt
     2701 taagtcagtt acaccaggtt gcgcatattg cacctcagta tcgccacctt gatatcgaag
     2761 ccctgaaaaa taatacggcg aacgatctac aactgatgca ggagattctc atgactttcc
     2821 agcatgaaac gcataaagat ctacccgctg cgtttcaagc actagaagct ggcgataaca
     2881 gaactttcca tcagtgtatt catcgcatcc acggtgcggc taacatcctg aatttgcaaa
     2941 agttgattaa tattagccat cagttagaaa taacacctgt ttcagatgac agtaagcctg
     3001 aaattcttca gttgctgaac tctgtaaaag aacacattgc agagctggac caggagattg
     3061 ctgttttctg tcagaaaaat gactaaatag cggctcccac aatgttcaaa tgtgggagct
     3121 atttaccagc acatcttaca tttatgatga gaactcctgg cgaatttgtt ccccatgctg
     3181 gtcgagcgtt gccgctcccg gcataacatg cgggtccgcg cagccgctga tttttatcgg
     3241 attacccggc atcattattc ccccggcttc aatcaacata tttctcgcct gagtttgtgg
     3301 caatttaatg gcctcagcca cacttaataa cggcgcgacg ggtacaccaa cttcatgtat
     3361 tctggctaac caaacttcag ctgcctgcgt ttttaacgtc cgctcaatat attgtttaag
     3421 aatagcctgg ttttgtacgc gtaaaatatt gctgctaaat cggggatcat taaccagttc
     3481 cgtaagctcc agtgcctggc ataacgcaga aaaaagcttg tcattaccac aacaaatcgt
     3541 aatcggctta tcctgagtat tgaaaacatc aaaaggtgcc atgtagggat ggcgatttcc
     3601 cagacgttgt ggtgacttcc ctgtcgcgat atatgccatc agaccatgct ccagaaaact
     3661 cagcgtggca tcaaacatcg ctatatcgac atgcgcccct ctctggctct tttcgcggcc
     3721 ataaagtgca ctcactattc cgctgaataa atagacaccg ccgcatagat ccgcaagaga
     3781 tgtaccaacg cgcactggcg gagcatcagg atatcctgtt tccatcatta tcccgctcat
     3841 tgcctgaatg atggtatcgt aggcaggagc atcttttagc ggaccggtat gtccgaaacc
     3901 tgacgatgaa gcatatatga ggcgcgggtt gatttcttga agcgtttccc atgaaaaccc
     3961 cagtttttcc attgtacctg ggcgaaaatt ctcagctaat acatcagctt gtttgagcat
     4021 atttataaat atacttttat cgtgatcatt ctttaaatca agaaccacac tctctttgcc
     4081 atgattaata aaactgtaat agagtgactg tccatccaca ta
//
LOCUS       DAZGAN020000223         4105 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGAN020000223 DAZGAN020000000
VERSION     DAZGAN020000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.224"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            224..1156
                     /gene="rfaD"
                     /locus_tag="ACHKYO_RS24795"
                     /old_locus_tag="pgaptmp_004959"
     CDS             224..1156
                     /gene="rfaD"
                     /locus_tag="ACHKYO_RS24795"
                     /old_locus_tag="pgaptmp_004959"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HGU4431627.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1166..2212
                     /gene="rfaF"
                     /locus_tag="ACHKYO_RS24800"
                     /old_locus_tag="pgaptmp_004960"
     CDS             1166..2212
                     /gene="rfaF"
                     /locus_tag="ACHKYO_RS24800"
                     /old_locus_tag="pgaptmp_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HGU4431628.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2216..3208
                     /gene="rfaC"
                     /locus_tag="ACHKYO_RS24805"
                     /old_locus_tag="pgaptmp_004961"
     CDS             2216..3208
                     /gene="rfaC"
                     /locus_tag="ACHKYO_RS24805"
                     /old_locus_tag="pgaptmp_004961"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HGU4431629.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3205..>4105
                     /gene="rfaL"
                     /locus_tag="ACHKYO_RS24810"
                     /old_locus_tag="pgaptmp_004962"
     CDS             3205..>4105
                     /gene="rfaL"
                     /locus_tag="ACHKYO_RS24810"
                     /old_locus_tag="pgaptmp_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGU4431630.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVY"
BASE COUNT         1073 a          948 c         1011 g         1073 t
ORIGIN      
        1 tgcttccgtt atttatcata catcggaata ttgatactaa agcactattc acatgcaaaa
       61 ccaacatccg ccatgaagga ctagctaaaa cccaaactag tttgttgcaa ttagcatcct
      121 tgcacctcta tgtaaagggc tgaagggatt cggatgtgat ggtatgatta cagacattcg
      181 tgtctgagat tgtctctgac tccataattc gaaggttaca gttatgatca tcgttaccgg
      241 cggcgcgggc tttatcggca gcaacatcgt taaagccctg aatgataaag gcatcaccga
      301 tattctggtg gtggacaacc tgaaagacgg caccaagttt gtgaacctgg tggatctgaa
      361 tatcgcagac tatatggata aggaagactt cctgatccag attatggctg gcgaagagtt
      421 cggcgatgtc gaagcgattt tccacgaagg cgcgtgctct tccaccaccg agtgggacgg
      481 caagtatatg atggataaca actatcaata ctccaaggag ctgctgcact actgcctgga
      541 gcgtgaaatc ccgttcctgt acgcttcttc cgcagccacc tacggcggac gcacctccga
      601 ctttattgaa tcccgcgagt acgaaaaacc gttgaacgtc tacggttact caaaattcct
      661 gtttgatgaa tatgttcgcc aaatcctgcc ggaagcgaac tcgcagattg ttggcttccg
      721 ctatttcaac gtttatggac cgcgtgaagg ccataaaggc agcatggcga gcgtcgcttt
      781 ccatctcaac acccagctta ataacggtga atcaccgaag ctgttcgaag gtagcgagaa
      841 ctttaaacgc gacttcgtct atgttggcga tgtggcagat gtaaacctgt ggttcctgga
      901 aaatggcgtt tccggcatct tcaacctcgg caccggtcgt gcggaatcct tccaggctgt
      961 agctgatgct acgctggctt atcacaagaa aggccagatc gaatacattc cgttcccgga
     1021 taagctgaaa ggccgctacc aggcgttcac tcaggcagat ctgacaaatc tgcgcgcggc
     1081 gggttacgac aaaccgttca aaaccgttgc tgaaggtgta acggaataca tggcctggct
     1141 gaatcgcgac gcataagagc tctgcatgaa aatactggtg atcggcccgt cttgggttgg
     1201 cgacatgatg atgtcgcaaa gtctctatcg cacgctccag gcgcgctatc cccaggcgat
     1261 aatcgatgtg atggcaccgg catggtgccg tccattatta tcgcggatgc cggaagttaa
     1321 cgaagctatc cctatgcctc tcggtcacgg agcgctggaa atcggcgaac gccgcaaact
     1381 gggtcatagc ctgcgtgaaa agcgctacga ccgcgcctac gtcttaccta actccttcaa
     1441 atccgcatta gtgcctttct tcgcgggtat tcctcatcgc accggctggc gcggcgagat
     1501 gcgctacggt ttactcaacg atgtacgcgt gctcgataaa gaagcctggc cgctaatggt
     1561 ggaacgctat atagcgctgg cctatgacaa aggcattatg cgcacagcac aagatctgcc
     1621 gcagccattg ttatggccgc agttgcaggt gagcgaaggt gaaaaatcat atacctgtaa
     1681 tcaattttcg ctttcatcag aacgtccgat gattggtttt tgcccgggtg cggagtttgg
     1741 tccggcaaaa cgctggccac actaccacta tgcggagctg gcaaagcagc tgattgatga
     1801 aggttatcag gtggttctgt ttggctcggc gaaagatcat gaagcaggca atgagattct
     1861 tgccgcttta aataccgagc agcaggcatg gtgtcggaac ctggcggggg aaacacagct
     1921 tgatcaagcg gttatcctga ttgcagcctg taaagccatt gtcactaacg attctggcct
     1981 gatgcacgtt gcggcggcgc tcaatcgtcc gctggttgcc ctgtatggtc caagtagccc
     2041 ggacttcaca ccgccgctat cccataaagc gcgcgtgatc cgtctgatta ccggctatca
     2101 caaagtgcgt aaaggtgacg ctgcggaggg ttatcaccag agcttgatcg acattactcc
     2161 ccagcgcgta ctggaagaac tcaacgcgct attgttacaa gaggaagcct gacggatgcg
     2221 ggttttgatc gttaaaacat cgtcgatggg cgatgttctc catacgttgc ccgcactcac
     2281 tgatgcccag caggcaatcc cagggattaa gtttgactgg gtggtggaag aagggttcgc
     2341 acagattcct tcctggcacg ctgccgttga gcgagttatt cctgtggcaa tacgtcgctg
     2401 gcgtaaagcc tggttctcgg cccccataaa agctgaacgc aaagcgtttc gtgaagcgct
     2461 acaagcagag aactatgacg cagttatcga cgctcagggg ctggtaaaaa gcgcggcact
     2521 ggtgacacgt ctggcgcatg gcgtaaagca tggattggac tggcaaaccg ctcgcgaacc
     2581 tttagccagc ctgttttaca attgtaagca tcatattgca aaacagcagc acgccgtaga
     2641 acgcacccgc gaactgtttg ccaaaagttt gggctatagc aaaccgcaaa cccagggcga
     2701 ttatgctatc gcacagcatt ttctgacgaa cctgcctaca gatgctggcg aatatgccgt
     2761 atttcttcat gcgacgaccc gtgatgataa acactggccg gaagaacact ggcgagaatt
     2821 gattggttta ctggctgatt caggaatacg gattaaactt ccgtggggcg cgccgcatga
     2881 ggaagaacgg gcgaaacgac tggcggaagg atttgcttat gttgaagtat tgccgaagat
     2941 gagtctggaa ggcgttgccc gcgtgctggc cggggctaaa tttgtagtgt cggtggatac
     3001 ggggttaagc catttaacgg cggcactgga tagacccaat atcacggttt atggaccaac
     3061 cgatccggga ttaattggtg ggtatgggaa gaatcaagta gagtgtcgtt ctacaagtat
     3121 gtctcttgca gatttgccag ctcaaaccgt ttttcaaaat ttaaatcttg aaataataac
     3181 caataagttg acatcggaga taagatgacc tcaacattat ttttctctct cgagaaaaaa
     3241 aactggatag cgtactggaa cagagctctc gtattcttat tcattaccac ctattttttg
     3301 ggtgggatta caaggtataa acatcttatt gttattctta tgacaataac gacaatcgtc
     3361 tatctctgca aacggccaaa acactatctc tcactattta aaacatttct ttttggtagt
     3421 gttgccatat taactattgc tgcattgctg tcacttcttc aatcccctga tgcaggtgct
     3481 agcatgaagg aaattttcaa agctattatt gagaatactt tactatgcac aatagcaata
     3541 ccgatcatat tgagagacga gaaaagagaa gatgtcgaaa aaatcgtttt cttctcattt
     3601 attagtgcgt tgggcttacg ctgtttttct gaattgatta cctattataa ggactatcaa
     3661 caagggataa tgccattcgc agattataga caccgtagca tttctgactc gatggtcttt
     3721 ttattccctg cattgttaaa tctctggctt atcaaatcag caaaataccg catttctttt
     3781 gtggttctaa gcgttatttt tatttttctg atattaggaa ctttatccag aggggcctgg
     3841 ctttccgtgt tagtcattgg attaatatgg attctgatgt ttaaacaatg gaagttacta
     3901 ttagtaggag taatggttgc catcattgca ttgtcggtta ttttcacaca taaggagatg
     3961 accgcaaagc taacgtataa acttcaacaa actaatagtt cttatcgcta tgcaaatggt
     4021 actcaaggca gcgcactcga tctaatatta gaaaatcctg ttattggtta tggttacggt
     4081 aacgttgcat ataaagatgt ctata
//
LOCUS       DAZGAN020000224         4099 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGAN020000224 DAZGAN020000000
VERSION     DAZGAN020000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.225"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            66..626
                     /locus_tag="ACHKYO_RS24815"
                     /old_locus_tag="pgaptmp_004963"
     CDS             66..626
                     /locus_tag="ACHKYO_RS24815"
                     /old_locus_tag="pgaptmp_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4431631.1"
                     /translation="MLIGYIRVSTNDQNTALQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDALMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYFPASVSEK"
     gene            717..2798
                     /locus_tag="ACHKYO_RS24820"
                     /old_locus_tag="pgaptmp_004964"
     CDS             717..2798
                     /locus_tag="ACHKYO_RS24820"
                     /old_locus_tag="pgaptmp_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4431632.1"
                     /translation="MNIMTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMT
                     FADCLISFNTRDCLWVRPVSGDPNVEITGAGIGAVIPLSADVAGTTEPSDWNNAETYT
                     SSAGDKVAGQTPSWYYVVVIAGQSNASSFGEGLPLPDTYDRPDPRIKQLARRNTVTPG
                     GVECAYNDIIPADHCLHDVLDMSNHNHPKADLSKGQYGCVGQGLHIAKKLLPFIPEEA
                     GILLVPCARGGSAFTDGADGEFTEASGATSASSRWGVNKPLFSDLVNRTKAALSSNPR
                     NILLSVVWMQGENDLKTGKHAEHSGLFVTMVETFRRELSAFSAQSPAGDMASVPWICG
                     DTTYYWKNTYPEQYKAVYGKYANEAKNIHFVPFVVDENGKNVPTNNPDEDPDIPGIGY
                     YGAISRDSTNWTSNDRASHFSSWARRGIISDRLATAILTLSGQKSAFFRGVTATEHSD
                     TGKSEPEPTGDVKDVSDQMKIIVSYTANAPTEPMKDQGWNAASGKATFVTDAAATGGN
                     KALRVNKAPNSMASWKMYHPITADNARDLFARGGDISLRFKIPSDAGIAENRYGVGIY
                     WAVSNWPDGDGKNNLIASCFLQTDSANLNVMYHKGSGSQQIGSYGVFDHEWHTLSLRF
                     AGGNSMSVTPVLDGKDGEPFELVKWVNAGFTVDQLTLTDITGNAATYPMLFDTIKVQV
                     NDAVSSSNVNK"
     gene            2834..3013
                     /locus_tag="ACHKYO_RS24825"
                     /old_locus_tag="pgaptmp_004965"
     CDS             2834..3013
                     /locus_tag="ACHKYO_RS24825"
                     /old_locus_tag="pgaptmp_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4431633.1"
                     /translation="MPILHTFMLYFCTCVCLLYLLSGGYKIIRNYFRNKIDAAAEEKI
                     KSGAVMPQPKDSGQI"
     gene            3049..3294
                     /locus_tag="ACHKYO_RS24830"
                     /old_locus_tag="pgaptmp_004966"
     CDS             3049..3294
                     /locus_tag="ACHKYO_RS24830"
                     /old_locus_tag="pgaptmp_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4431634.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGHPPAENSTENEPQPVEQPEPVQPGTDSAMM"
     gene            3369..3569
                     /locus_tag="ACHKYO_RS24835"
                     /old_locus_tag="pgaptmp_004967"
     CDS             3369..3569
                     /locus_tag="ACHKYO_RS24835"
                     /old_locus_tag="pgaptmp_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431635.1"
                     /translation="MDKNKKQPDTKGLEILSAEIINALRSGLDENTLQDRNFTTAMWI
                     AMQILCTPRCAIFAVREGQLTR"
     gene            complement(3756..3866)
                     /locus_tag="ACHKYO_RS24840"
                     /old_locus_tag="pgaptmp_004968"
                     /pseudo
     CDS             complement(3756..3866)
                     /locus_tag="ACHKYO_RS24840"
                     /old_locus_tag="pgaptmp_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3889..4099)
                     /locus_tag="ACHKYO_RS24845"
                     /old_locus_tag="pgaptmp_004969"
                     /pseudo
     CDS             complement(3889..4099)
                     /locus_tag="ACHKYO_RS24845"
                     /old_locus_tag="pgaptmp_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1135 a          865 c         1021 g         1078 t
ORIGIN      
        1 gtttcaggga tgccgtatat ctgctttaaa aagcattttc acctcttttt aaggaaggta
       61 aatacatgct gattgggtat atcagggtgt caacaaatga ccagaatacg gcgctacaga
      121 gaaatgcgct ggaatgtgca ggatgtgagc agatttttga agataaaatg agcggaaaaa
      181 caacgcagcg gccgggactg aagcgggcac tgaagaaact aaaacccggt gacgcactga
      241 tggtctggaa actggacagg ctggggcgca gtatgcgcca ccttgttgcg ctgacagatg
      301 aattacagca acaggggata aattttcgta gtcttactga caacattgat acgtcaaccc
      361 ccatggggcg cttctttttt cacataatgg gggcgctggc agaaatggag cgtgaattga
      421 ttgtcgaacg gacacgggct ggtcttgcag ctgcccgtga aaggggacga accggcggca
      481 gaccccgtcg tctcacgcag gaacaatacg agcaggtcgg gcgtttactg gccgggggtg
      541 tcagccgaag acaggtggca cttatttttg atatcggcct ttcaaccctt tatcgttatt
      601 ttcctgcgag cgtttcggaa aaataagacg ctccctttgt tgggccttta ctgaattaac
      661 gcaaaatggc gtttatcggt ctgtggatat gataattctg tttattcagg aacaggatga
      721 atattatgac gtccaaatgg gttcaactat catcaatgcc cggaaacttt accgttaaag
      781 tttccggtgg tacagcggca tttcttgagg ctccctttcc tccggctgaa acgaaaggag
      841 gtatgacatt tgctgactgt ctcatcagtt ttaatacacg ggattgcctg tgggtaaggc
      901 cagtatccgg tgatccgaac gtggaaatta ccggagcggg tattggtgct gtcattccgt
      961 taagtgctga tgttgccggt actactgaac cgtcagactg gaacaatgcg gaaacataca
     1021 cttcttcagc aggagataaa gttgcaggtc aaactccgtc ctggtattac gtggttgtta
     1081 ttgctggtca gtcgaatgca tcgtcatttg gtgaaggtct tcctttgcct gatacctatg
     1141 atcgacctga tccacgtatt aaacagctgg ctcgccgtaa cacggttaca cctggcgggg
     1201 ttgaatgcgc ctacaacgat ataattccgg cagatcactg tcttcatgat gtgctggata
     1261 tgagtaacca taatcatcca aaagcagatc tttcaaaagg tcagtatggt tgtgtcggcc
     1321 agggtttgca tattgcaaaa aaactcctgc cctttatccc ggaagaggct ggtatccttc
     1381 tggttccctg tgccagagga ggctctgcat ttaccgatgg tgctgatggt gaatttactg
     1441 aagcttccgg agcaacatct gcatcatcgc gctggggagt caataaacct ttgttcagcg
     1501 atcttgttaa ccgaacaaaa gcggctctca gcagtaaccc cagaaatatt ctcctgtccg
     1561 ttgtctggat gcagggcgag aatgatctga aaacaggaaa acatgctgaa cattccggtc
     1621 tgtttgtgac gatggtcgaa acattcaggc gtgaactttc ggctttttct gcgcaatctc
     1681 cagccggaga tatggcttct gtaccgtgga tctgtggtga tacgacgtat tactggaaaa
     1741 atacatatcc agaacaatac aaagctgttt atggcaaata tgctaatgaa gcaaagaaca
     1801 ttcactttgt tccatttgtt gtggatgaaa atggaaaaaa tgtaccgaca aataatccag
     1861 atgaagaccc ggatattcca ggtattggct attatggtgc cataagtcgc gattcgacta
     1921 actggacatc aaacgacagg gcaagccatt tcagttcatg ggcgcgccgt ggaattattt
     1981 cagaccgtct ggcaacagcg attctgacat tgtcaggaca aaaatctgct ttcttccgcg
     2041 gtgtaacggc aacagaacat tctgataccg gtaagagtga gcctgaaccc accggggatg
     2101 tgaaggacgt gagcgatcag atgaagatca tcgtttcgta tactgcaaac gccccgacag
     2161 aaccaatgaa agatcagggg tggaatgcgg cttccggaaa agcaaccttt gtcacggatg
     2221 ctgctgcaac aggtggtaac aaagcgttaa gggtgaataa agcgcctaac agcatggcct
     2281 catggaaaat gtatcatccg atcacagctg ataatgccag ggatttgttc gccagaggtg
     2341 gtgatatttc cctccgtttc aaaattccat cggatgcggg cattgcggaa aaccggtatg
     2401 gtgttggtat ttactgggct gtaagtaact ggcctgacgg tgacggaaaa aataacctca
     2461 tcgcatcatg tttcctgcaa acggattcag ctaacctgaa tgtgatgtat cacaaaggga
     2521 gcggaagtca acagatagga tcgtatggtg tctttgatca tgaatggcat acgctctcgt
     2581 tacggtttgc aggtggtaat agcatgagtg ttaccccagt tctggacggg aaagatggtg
     2641 aaccatttga acttgtgaag tgggtgaatg ccgggttcac ggtggatcaa ctgacactga
     2701 ccgatattac cggcaatgca gccacttacc cgatgttgtt tgacacaatt aaagtgcagg
     2761 ttaacgatgc ggtctcttcg tctaatgtga ataaatgacg ggtaattcat tttatttaaa
     2821 atggtgagtg attatgccaa tactacatac atttatgctt tatttttgca cgtgtgtgtg
     2881 tctgctttat ctcctcagcg gaggttacaa aattatccga aattattttc gaaataaaat
     2941 tgatgcagcc gcagaggaaa agataaaaag cggtgctgta atgccgcagc cgaaagactc
     3001 cggtcagatt taattttttg tgaaccagaa aaggaaagga gaatattcat gccagagatt
     3061 aaaggcacgg ttactgaaga gcttgtcaaa caggcacttt attctgaaga agtgaaccgc
     3121 gtgctgaagg cgcaggttcg caaggatttt gaggcacaaa tcgacgcata tgttgatgaa
     3181 gtgctggccc gaatggttgg ccatcctccg gctgaaaaca gcacggagaa tgagcctcag
     3241 cccgtagagc aaccagagcc ggttcagccc ggaactgaca gtgccatgat gtaacaatac
     3301 aaaccgacag cttctcctgt cggtttgatt ttcatgggcg atgtatatac cagtcagagg
     3361 aaataatcat ggacaagaac aaaaagcagc ccgataccaa aggtctggaa atattaagcg
     3421 cagaaattat caatgcgcta cgttcaggac ttgatgaaaa tacattacag gatcgaaact
     3481 tcaccactgc aatgtggata gcgatgcaga tcctgtgtac cccaagatgc gctatttttg
     3541 cggttcgtga gggtcagttg actcgctgat tgcccgaatt acatcagaaa agcacgcgtt
     3601 ccataattgc atagctgatg aatcaccagc tgtatgaaaa gaggtaaaga cacaactggt
     3661 ggatgtcatt gctgcaattg tgatatcagc tgcaattcgc gcagcctgta acttttggtc
     3721 ggtggtcagt ttatccattt tctcctgcct gaacagatgc atctctgctt tacaggaggc
     3781 cggggaatat cataggatac accacgagct cagagtgaag cattgatgca gttttttaca
     3841 gattgaccca gaatagttcg cgctatctga gtcgccattt atgtagtccc gaagtaaagc
     3901 attgatgcaa acttgtctaa atcggcccag catagttcca gctatgctgg gtttcccttt
     3961 tatgtagtat cctgttgatt tgaaacatac tacatatacg cgcgatccta ccttcactgg
     4021 ataaaaagtc aataaaatga tcattttatg atcgttttca ccacctaact cttcgttttc
     4081 tttaagtaaa taatcacct
//
LOCUS       DAZGAN020000225         4098 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGAN020000225 DAZGAN020000000
VERSION     DAZGAN020000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.226"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(156..1121)
                     /locus_tag="ACHKYO_RS24850"
                     /old_locus_tag="pgaptmp_004970"
     CDS             complement(156..1121)
                     /locus_tag="ACHKYO_RS24850"
                     /old_locus_tag="pgaptmp_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4431636.1"
                     /translation="MLFVGSQKHSALSIFAKTTRMSALVLCGNSGVILLSVKDHQHID
                     SAIPGRYTVQAPYKTGAGIGVLKLITEHNRAHAVFSCHEHYYAQIMVGRTGPTSVGPG
                     SVVSGNANPVRLTTSVIGVPCGEFSKKLTTGAVTMTTLPTLSQPEIAIVDGQAVTSSL
                     AVADFFSKRHDDVLKKIRILDCSPEFCARNFAETSILVRQPNGGTRKLPCYQITRDGF
                     AFLAMGFTGKRAARFKEAYINAFNQMEKQLSKPSTLSDAAHNASVLYSHLSVIHKVWL
                     QQLYPMLAKVESPLAISLYDHINDAAAISSLINMTLNPSEVRGRK"
     gene            complement(1075..1170)
                     /locus_tag="ACHKYO_RS24855"
                     /old_locus_tag="pgaptmp_004971"
                     /pseudo
     CDS             complement(1075..1170)
                     /locus_tag="ACHKYO_RS24855"
                     /old_locus_tag="pgaptmp_004971"
                     /inference="COORDINATES: protein motif:HMM:NF025339.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            complement(1316..1867)
                     /gene="ymfL"
                     /locus_tag="ACHKYO_RS24860"
                     /old_locus_tag="pgaptmp_004972"
     CDS             complement(1316..1867)
                     /gene="ymfL"
                     /locus_tag="ACHKYO_RS24860"
                     /old_locus_tag="pgaptmp_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HGU4431637.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAAGTHYIADAVAQSAGGVFVSLPDIEEVENADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEQTAMNDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            complement(1860..2120)
                     /locus_tag="ACHKYO_RS24865"
                     /old_locus_tag="pgaptmp_004973"
     CDS             complement(1860..2120)
                     /locus_tag="ACHKYO_RS24865"
                     /old_locus_tag="pgaptmp_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4431638.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIDL
                     AERLANFFKGEISEMQILYPARFQSSQNRNELKPQEKEVSRG"
     gene            2218..2910
                     /locus_tag="ACHKYO_RS24870"
                     /old_locus_tag="pgaptmp_004974"
     CDS             2218..2910
                     /locus_tag="ACHKYO_RS24870"
                     /old_locus_tag="pgaptmp_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4431639.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQALSNIARSLGVDIADLFTSDFKSNTVCKNSTGEDVAQVKDVFRIEMLDVSASAGNG
                     LIQGGDVIDVIHAIEYITDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDIS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            3690..4052
                     /gene="yfdP"
                     /locus_tag="ACHKYO_RS24875"
                     /old_locus_tag="pgaptmp_004975"
     CDS             3690..4052
                     /gene="yfdP"
                     /locus_tag="ACHKYO_RS24875"
                     /old_locus_tag="pgaptmp_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HGU4431640.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
BASE COUNT         1117 a          997 c          996 g          988 t
ORIGIN      
        1 gcggtaatta cggcccagcg gttcacaaat caccttctga cattcacggt caaccaggct
       61 aacacgtaga acatgccctg caggcgtggt gtaccactga cccgggcgag gacaacggaa
      121 agtctgatcg gtaaaccgtt tgaaaatatt ccggatcatt tgcgccccct tacctctgaa
      181 gggttcagtg tcatattgat taggctggaa atcgcagcag catcgttgat atggtcatac
      241 aggcttatag ccagcggaga ttccactttg gccagcatgg gatagagttg ttgcagccag
      301 accttgtgga ttaccgacag gtgggagtaa agcacgctgg cgttatgtgc ggcatcgctc
      361 agcgtggatg gctttgaaag ctgcttttcc atctggttaa aggcgttgat gtacgcctct
      421 ttaaaccggg cggcgcgttt gccagtgaag cccattgcca ggaacgcgaa gccgtcgcgg
      481 gttatttgat agcaaggaag tttgcgagta ccgccgttgg gctggcgtac caaaattgat
      541 gtctccgcaa aattgcgggc acaaaactct ggagaacaat ccaaaatgcg gatctttttc
      601 agaacatcgt catgacgttt agagaaaaag tcagcaacag ccaaagaaga tgtaacagcc
      661 tgaccatcaa cgatggcaat ttcaggttga gagagggttg ggagagtagt catagtgaca
      721 gccccggtag tcagtttttt agaaaactca ccacatggga cgccaatcac agaggtggtg
      781 agacgtacag ggttggcgtt accggagact accgaacccg gcccgaccga agtcggccct
      841 gtacgcccca ccataatttg ggcgtagtaa tgctcatgac acgaaaaaac cgcatgagcg
      901 cggttatgct cagtaatcaa tttcaggacg ccaatcccgg cacccgtttt ataaggtgcc
      961 tgaacagtgt aacgtcccgg aattgcagaa tcaatatgct ggtggtcctt cacactcaac
     1021 aaaatcacgc ctgaatttcc acaaaggact aaagcactca tgcgggtagt ctttgcgaag
     1081 atagataacg cgctgtgttt ctggctccca acgaataaca tgaacataaa gtcctcttcc
     1141 gtcacgaaac cagcggttaa gttcctgcac aactcgcccc ccacagtcag gtaaagttct
     1201 ctgtggttac ttacagccag gtgatttggt aatctgcatt catgccgtaa caacaggtgt
     1261 gcagcgacac tgaccaccag ctgttgcgac aaacggttat ttgccgttaa actgttcatg
     1321 cgttagtttc tccacagaca caaaacgcca cgacgcccgg agctgcacac tcgcgggcgt
     1381 cactcttttc tggagcgcag aaaattttgt agaccagtgc cgcatgctcc tggagcttcg
     1441 aaattgacag atacaactca tcattcattg ctgtctgctc gtgtggctcc actaccccat
     1501 cttcgattgc cgaacgaatc tgctttgagt aactcccgat ctgttcgatg acttccagca
     1561 ggcgctggtt tatatcggcg ttctctactt cctcaatgtc aggaagcgat acaaacaccc
     1621 caccagcaga ctgtgcgaca gcatccgcaa tgtagtgagt gccagccgcg cgctgtaaaa
     1681 tcattgccca tcccagcggg aaaatctgat cgccatcggc acgaaggcga ttgaataaag
     1741 cgttctctgt tacatccagc cactcagctg cttcagcgta accacccggc aacgccgcga
     1801 tagtttttct gaccgctttc acgtaccact caggctgttt ttccactttc cagtgatgct
     1861 tacccacggc ttacctcctt ttcctgtggt tttaactcat tccggttttg actagattga
     1921 aagcgagcag gatagagaat ctgcatttcg ctgatttctc ccttaaaaaa attggccaga
     1981 cgctctgcca gatcgataga tggaatttgt tccagtctct caatacgact cagcgtcgct
     2041 ggattaacct gaacacccgc agcaacatgc tgcaaagtaa atccgtgcgc cttacgcaca
     2101 tttcgtaatg gtgattgcat ataacctcca catattgcgt gataagcata ttatttcacg
     2161 caaatatttt gcgcaagttg atttgcttaa cgcgcaataa agaaatgtaa taaacgcatg
     2221 aacataggaa atcgagtcag acaacttcgc caggcgaaga acatgaaaat cgccgatctc
     2281 gctgaagcaa taggagtgga tgcggcgaat atctcacgcc tggaaacagg taagcagaaa
     2341 caattcactg aacaagccct gagtaatatt gccaggagct taggtgttga tattgcagat
     2401 ctctttacct cagacttcaa aagtaatact gtatgtaaaa acagtactgg tgaggatgtt
     2461 gcgcaggtga aggatgtatt ccgtattgaa atgctggatg tcagtgccag tgcgggaaat
     2521 ggccttatcc agggcggtga tgtcattgat gtgattcatg ccattgaata cataactgat
     2581 aatgctgtat cgatgtttgg aggacgacca gccaatcaca ttaaagttat caacgttcgt
     2641 ggggacagta tgtgtccaac cattgagcca ggagatctca tcttcgttga tatcagtatc
     2701 aatcagtttg atggggatgg tatctatgtg tttggttttg atgataaaat ttatgtcaaa
     2761 cgactgcaaa tgatacctga caaactactg gtgatttctg ataaccagat ttaccgtgaa
     2821 tggggaatta ccagcgaaaa cgaacaccgg tttatggtct ttgggaaggt cttaatcagt
     2881 cagtcacaaa cccttaagcg acacaattaa ccccgacctc ctcatcaatt agccaccaga
     2941 aggtggcttt tcatcaccca tcatattgcg catctcgcaa caaaaacact tgcataatgc
     3001 gcaatttcat tttatctttc tttccagacc aacaaacaag gtcctaacaa aatttggttg
     3061 taacacggcg tatggcacat gcgtcgttag cggtctgggg acgttaaagg ggacaatcca
     3121 ctccttgctc gggcaaacaa accaggtagc cggaatgtgc aagtcaatga ggatgctgat
     3181 aagacgccta accagcgtgg cgattcggtt tgacgcctgg gaagagacca ggacgcaacg
     3241 atgagagcat tgacgagcaa ggcataagtg ctggttcaat tccagacagt cccattcaga
     3301 tgggagggtt gggcagggaa aaggtccgtt cgattcggac accggcaatg ctctcagcgt
     3361 tgtggtgaat gcgcaggctg atgcgcgaaa gacattgcag ctattgcgga aaagagctgt
     3421 tcggcggggc aatcaaacgc ccgtgagagt ctgaaataac cgcaagccgg agatcagcac
     3481 cggtcaccac aacagccact gctttggcag taccagtttg tacacttgct tccggctggt
     3541 accgctcttt ttacaaaaca gagaagagca tcaccggacg acgggctcat aacccaatcc
     3601 atccgggcgg ctgccaccgc aggtgttctt ctctgttttg tggagaaacc aaccgacctt
     3661 gcagggtcga tatgatgagg agcaacaaaa tggctagcga acgcagtact gatgtgcagg
     3721 catttatcgg ggagctggac ggcggcgtat ttgaaacaaa aatcggcgca gttctcagtg
     3781 aagtcgcttc cggtgtgatg aacacgaaaa ccaaaggtaa ggtctcgctc aacctggaaa
     3841 tcgaaccgtt tgatgagaac cgtgtgaaaa tcaaacacaa actctcatat gttcgcccga
     3901 ctaaccgcgg gaaaatttcc gaagaagaca ccaccgaaac gccgatgtat gtcaatcgcg
     3961 gtggtcgcct gactattctg caggaagacc agggacaatt actgactctt gccggtgagc
     4021 ctgacggaaa actccgcgca gcaggtcatt aatatcgttc ttaattaact gattatttat
     4081 ctcatcactg aatatctt
//
LOCUS       DAZGAN020000226         4096 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGAN020000226 DAZGAN020000000
VERSION     DAZGAN020000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.227"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1286..2329
                     /gene="subA"
                     /locus_tag="ACHKYO_RS24880"
                     /old_locus_tag="pgaptmp_004976"
     CDS             1286..2329
                     /gene="subA"
                     /locus_tag="ACHKYO_RS24880"
                     /old_locus_tag="pgaptmp_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HGU4431641.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHG
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWTELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSMNKKRDPVIRV
                     AALAQYRKGETPVLHGGGITGSRFGNGWVDIAAPGQNITFLRPDGKTGTGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEGRVLNAEKAISMFCKKN
                     YIPVRQGRMSEEL"
     gene            2346..2768
                     /gene="subB"
                     /locus_tag="ACHKYO_RS24885"
                     /old_locus_tag="pgaptmp_004977"
     CDS             2346..2768
                     /gene="subB"
                     /locus_tag="ACHKYO_RS24885"
                     /old_locus_tag="pgaptmp_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HGU4431642.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVWTYPPFVKALTSNALVGLSTCATSTECFGPDRKKN"
     gene            complement(3083..4069)
                     /locus_tag="ACHKYO_RS24890"
                     /old_locus_tag="pgaptmp_004978"
     CDS             complement(3083..4069)
                     /locus_tag="ACHKYO_RS24890"
                     /old_locus_tag="pgaptmp_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4431643.1"
                     /translation="MYRYDFNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRV
                     KQLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHI
                     AKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLV
                     ARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCV
                     GNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEP
                     EEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
BASE COUNT         1145 a          897 c          881 g         1173 t
ORIGIN      
        1 ttttcacaaa gtaaatttaa aaacaaagaa atttaatgta atataatcgg gggagagtta
       61 gccatcgatg attgccggaa caaaaaacaa tataaaccaa caagatagaa aatcatcaca
      121 acaaagggaa aggtctgtgg ataagagggg gaggaacttt ctgcttcatg tcacataatt
      181 gatatacttt atcccgcaac ctccagttgc attatatata attcaacctc aggctcccat
      241 cacaggcaaa aaataatata tcgcaagccg catcaaatcc ggatttttat ataataaata
      301 atacctgttt tttccatcac cagtctgtta atcaacttaa tatatttcag ggttatttta
      361 cattagcccg gcaaaagtta tcaggcatgc aggatacatt atcagggaga gcggtagtga
      421 acgccggtac ctgcttcccc aaagttattt aattaacccg tatcaacagg gtaacagcat
      481 ggcaaaatcc agcaattacg cacctgttaa ggtcattatt tacgttgata tcggtaaaga
      541 tccctgccac actgttatca ttaatcgttc tgataattag ttcgacaaaa tattagcaaa
      601 cgtcgaaaac aagctcagga aacagatatc cggcctcagg aaatatgggc atatactgct
      661 gattgctgac cagcctgcta ccatcgttgt attacccgtc cacattgcac gctcagaagg
      721 tgtccttgtc agatacctgt aagtggactg actgccccgt attgatatct attgaccagt
      781 cagttcgact ttcccgggga gcagctcgca tccccggttt aacggtcatt catgaatata
      841 ctcagtgacc tgatatccgg ggcttaccaa tgaaccacac caccattcac tgattgcccc
      901 ggtaaaacgg cgctgtgata tcctgttcgc catgatgcct gacggaactt tttattgtca
      961 taaacatgcc aggcaactta cttgcctcct cttatttttt ctggttaagt tttatccatc
     1021 atggctgtat ttacttctgc tctgaaggag gaggcagagc atcttatcgc tgacctgttg
     1081 cagaaatcca gatgcatatt accgtgaagg tgagcgttaa ttcattggat acataaatat
     1141 aaaaattaat gtttttgaga cgggcttcac tcgctttatt ggttgtgatt gtttatcttt
     1201 tgtcgaaaac aggatatttc agatgtgatg ttcctgtaag atattgacct gcaatactgt
     1261 ttatttgtac tgactggagg atattgtgct taagatttta tggccgcata ttctgttcct
     1321 gctttttata tctgtgtctg tcagggcaga aaaaccctgg tattttgatg caattggcct
     1381 gacagaaaca accatgtcgc tcacagacaa gaatacccct gttgtcgtat ctgttgttga
     1441 ttccggagta gcatttgtag gaggcctcag cgacagcgaa tttgccaaat tcagttttac
     1501 tcaggatggt tcgccattcc cggtaaaaga gtctgaagct ttatatattc atggtactgc
     1561 catggcttca ctcattgcct cacgtcatgg ggtttatggt gtttatcctc atgcactgat
     1621 atccagcaga agggttattc ctgacggtgt acaggactca tggattagag caactgaaag
     1681 cattatgtca aatgtttttc ttgcaccagg agaagagaaa atcattaata tatcgggagg
     1741 ccagaagggg atatcttccg catcggtctg gaccgaactg ctttcccgta tgggccggaa
     1801 taatgagcga ttaattgttg cggcagtggg taatgatggc gcagatatac gaaaactgag
     1861 tgctcagcag agaatatggc ctgcggctta ccatccggtc agcagtatga ataaaaagcg
     1921 ggatcctgtg ataagagtgg cagctctggc acagtaccgg aaaggagaaa cacctgtatt
     1981 gcatggtgga gggatcaccg gaagtcggtt cggaaacggc tgggttgata ttgctgcacc
     2041 gggacagaat attacattcc tcagaccaga tggaaaaacg gggacaggta gtggaacctc
     2101 ggaagcaaca gctatagttt ccggcgttct ggcagcgatg gtctcatgta atccccgtgc
     2161 aacagcgaca gaactgaagc gtacactgct ggagtctgca gacaaatacc cctcacttgc
     2221 agataaggtg acggaaggga gggttcttaa tgcagaaaaa gcaattagca tgttctgcaa
     2281 gaaaaattat attcctgtcc gtcagggaag gatgagtgaa gaactgtaaa attccggagg
     2341 tgaatatgac gattaagagg ttttttgtat gtgcaggtgt tatgggatgt ttatccctta
     2401 atcctgccat ggctgagtgg actggcgatg cccgggatgg catgttttca ggcgttgtta
     2461 ttgatcagtt tcatacaggg caaatagata ataaccctta tttttgtatt gaagggaaac
     2521 aacctggtgg ttcttccata agagcctgct cgatgaagaa ttcatctgtc tgggggcctt
     2581 cgttttccac attatataat caggcactat atttttacac aacaggtcag ttggtcagga
     2641 tttattatga acccggggta tggacgtatc cgccctttgt gaaggcatta acgtccaatg
     2701 cccttgttgg gttatcgacc tgcgccacat cgactgaatg ttttggccct gacagaaaaa
     2761 agaactaata ggtgatgttt ccgtccagag atactctgga tggagtgtcg gcgtcagtga
     2821 tataagactc gagtgaacac tgagccgacc tcgaaatgac gttgtaggtg cagacatgcc
     2881 ataatgggac tgtcgatgat attacaggga ctcacgtccc tggtgcgtgc cttcgtgaca
     2941 cactaccgtc cacatagtat cagaaccagt gatatcaaac ggtaacgacc agctatcttg
     3001 gtgttgatac cgacaactcc ataccactcc gtcatgtctc tcagagaatc atgtcagagc
     3061 tgtagatata tcctgaactt gtctacaaat aatttcttat tttttgcgca taacattcag
     3121 caataactcc ccttctggcc attgaattaa agtgtgtact tctctgtgac gttgtccagc
     3181 ttaattctgt cctccatgat gaccccagat acccgacaga taaatcatcg gcatcttctt
     3241 ctggctcatt agttaattca tgtcggttac tatcctgaaa gctgagaaaa tgtatttcat
     3301 catcagtccg ttgtctgtaa tgtccatata taaaatcata ttctttttga taagattcct
     3361 tccagtacca ggttgtatca ccacacaacc atggaactct ttctgacgaa ttaccaacac
     3421 actgagatga gtattcagat aactcagacc tgaaagaagt taccatctga taaaacaagt
     3481 caggatgctt ttcataatct ggtgacataa gatcaaactc cccctgcatc cagcagacag
     3541 atagcagttt attctttctg ttatattcca gagctacctt aacccgggct actaagtcct
     3601 catagagtgc tgttccggtg ccccaccgca ttgcatcagc tgttgcccct gtatcaggta
     3661 catacatacc ttcagctcca acagtaaagg cggcacctcc tctgcaacaa ggaaccagta
     3721 atatccctgc ttgctcaggt atgtatggca gtaacttttt agctatatgc aatccctgtc
     3781 cgacacaacc atattcccct ttatgcaaat cagcagccgg atggtgatat ccactcatat
     3841 cctgaacatc atgcagacag tgatctgccg gaataatctc attgaattta cactcttttc
     3901 ctcctggtgt gattgttttt cgacgagcca gttgttttac acgggactcg ggtttgtcga
     3961 gtgtatctgg caggggaata ccttcaccgt aggccatacc attagactgt cctgcgaggg
     4021 ctatcacata ccagaaatct attttttcat taaaatcata acgatacatc actcccccct
     4081 ttacataata gattta
//
LOCUS       DAZGAN020000227         4006 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGAN020000227 DAZGAN020000000
VERSION     DAZGAN020000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.228"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            241..501
                     /locus_tag="ACHKYO_RS24895"
                     /old_locus_tag="pgaptmp_004979"
     CDS             241..501
                     /locus_tag="ACHKYO_RS24895"
                     /old_locus_tag="pgaptmp_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HGU4431644.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            725..799
                     /gene="tap"
                     /locus_tag="ACHKYO_RS24900"
                     /old_locus_tag="pgaptmp_004980"
     CDS             725..799
                     /gene="tap"
                     /locus_tag="ACHKYO_RS24900"
                     /old_locus_tag="pgaptmp_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HGU4431645.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            792..1649
                     /gene="repA"
                     /locus_tag="ACHKYO_RS24905"
                     /old_locus_tag="pgaptmp_004981"
     CDS             792..1649
                     /gene="repA"
                     /locus_tag="ACHKYO_RS24905"
                     /old_locus_tag="pgaptmp_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HGU4431646.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRAQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVVWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            2350..2543
                     /locus_tag="ACHKYO_RS24910"
                     /old_locus_tag="pgaptmp_004982"
                     /pseudo
     CDS             2350..2543
                     /locus_tag="ACHKYO_RS24910"
                     /old_locus_tag="pgaptmp_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2585..2914
                     /locus_tag="ACHKYO_RS24915"
                     /old_locus_tag="pgaptmp_004983"
     CDS             2585..2914
                     /locus_tag="ACHKYO_RS24915"
                     /old_locus_tag="pgaptmp_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4431647.1"
                     /translation="MFSVVYHPEAREEATALPIKIRVKFDRLISKLEYDARLLREPDT
                     KPLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEV
                     LNETGKP"
     gene            2895..3176
                     /locus_tag="ACHKYO_RS24920"
                     /old_locus_tag="pgaptmp_004984"
     CDS             2895..3176
                     /locus_tag="ACHKYO_RS24920"
                     /old_locus_tag="pgaptmp_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4431648.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDN
                     LTKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVITLY"
     gene            3359..3643
                     /locus_tag="ACHKYO_RS24925"
                     /old_locus_tag="pgaptmp_004985"
     CDS             3359..3643
                     /locus_tag="ACHKYO_RS24925"
                     /old_locus_tag="pgaptmp_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HGU4431649.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVISGQIIDHQAVQAWADSLSTDNPLPVPR"
     gene            3643..3918
                     /locus_tag="ACHKYO_RS24930"
                     /old_locus_tag="pgaptmp_004986"
     CDS             3643..3918
                     /locus_tag="ACHKYO_RS24930"
                     /old_locus_tag="pgaptmp_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4431650.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARMVQSLIQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
BASE COUNT         1085 a         1002 c         1000 g          919 t
ORIGIN      
        1 ggtatttcag gggcagacac ccgacatgcc agaaaccgcc ggtcccgccc ggggccggca
       61 cacaggttaa ggcatttcct gtttttcagc catttcgcaa caaaaatcac attaaacaac
      121 agtaatcacc gttgatttgt gcgccaacac agatcttcgt cacaattctc aagtcgctga
      181 tttcaaaaaa ctgtagtatc ctctgcgaaa cgatccctgt ttgagaattg aggaggcgag
      241 atgtcgcaga cagaaaatgc agtgacttcc tcattgagtc aaaagcggtt tgtgcgcaga
      301 ggtaagccta tgactgactc tgagaaacaa atggccgctg ttgcaagaaa acgtcttaca
      361 cacaaagaga taaaagtttt tgtcaaaaat cctctgaaag atctcatggt tgagtactgc
      421 gagagagagg ggataacaca ggctcagttc gttgagaaaa tcatcaaaga tgaactgcag
      481 agactggaca tactaaagta aagactttac ttttggcgta gcatgctaga ttactgatcg
      541 tttaaggaat tttatggcgg gccacgccgt aaggtggcag ggaactggtt ctgatgtgga
      601 tgtacaggag ccagaaaagc aaaaaccccg ataatcttct ttaactttgg cgagtcagaa
      661 agattaccgg ggccatctaa aaaccgtata gccaacaatt cagctatgcg gggagtatag
      721 ttatatgccc ggaaaagttc aagacttctt tctgtgctcg ctccttctgc gcattgtaag
      781 tgcaggatgg tgtgactgat cttcaacaaa cgtattaccg ccaggtaaag aacccgaatc
      841 cggtgtttac accccgtaaa ggtgccggaa cgctgaagtt ctgcgaaaaa ctgatggaaa
      901 aggcggtggg cttcacctcc cgttttgatt tcgccattca tgtggcgcat gcccgttcgc
      961 gtggtctgcg tcgacgcatg ccaccggtgc tgcgtcgacg ggctattgat gcgctgctgc
     1021 aggggctgtg tttccattat gacccgctgg ccaaccgcgc ccagtgttcc attaccacac
     1081 tggccattga gtgcggactg gcgacagagt ccggtgcagg aaaactctcc atcacccggg
     1141 ccacccgggc cctgacgttc ctgtcagagc tgggactgat tacctaccag acggaatatg
     1201 acccgcttat cgggtgctac attccgaccg atatcacgtt cacaccggcg ctgtttgccg
     1261 cccttgatgt gtctgaggat gcagtggcag ctgcgcgccg cagccgtgtg gtatgggaaa
     1321 acagacagcg caaaaagcag gggctggata ccctgggtat ggatgagctg atagcgaaag
     1381 cctggcgttt tgtccgtgag cgtttccgca gttaccagac agagcttaag tcccacggca
     1441 tgaaacgtgc ccgtgcgcgc cgcgatgcag gcagaacccg ccaggacatt gtcaccctgg
     1501 tgaaacggca gctgacgcgt gaaatcgcgg aagggcgttt ccggggaagc ctggaggccg
     1561 tgaagcgcga aatcgaccgt cgtgtgaagg aacgcatgat aatgtcgcgt aacaacaact
     1621 acacacggct ggccactgcc tccccctgag ttttacctca tctgaataat ccggcctgcg
     1681 ccggaggcat ctgcacgcct gaagcctgtc agcgaacaaa aaaacagcac cacatacaaa
     1741 aaacaatctc atcatcccgc ttctggtgca tccggccccc ctgttttcga tacaaaacat
     1801 gcctcactga cggggaattt tgcttatcca catttaactg caagggactt ccccataagg
     1861 ttacaaccgt tcatgtcata aagcgccagc cgtcagcgtt acagggtgca atgtatcttt
     1921 taaacacctg tttatatctc ctttaaacta cttaattaca ttcatttaaa aagaaaacct
     1981 attcattgcc tgtcctgtgg acagacagat atgcacctcc caccgcaagc ggcgggcccc
     2041 aaccggagcc attttagtta caacactcaa atcaaccacc gggaaaactc cggtccagcg
     2101 cagaaccgaa accacaaagc ccctccctca taactaaaaa gcggccccgc cccggcccaa
     2161 agggccggaa cagagtcgct tttaattatg aatgttgtaa ctatactcca tcatggctgt
     2221 cagtcttatg gcgggaagtt ctcagtacac gctcgtaagc ggccctgacg gcccgctaac
     2281 gcggagatac gccccgactt cgggtaaacc ctcgtcggga ccactccgac cgcgcacata
     2341 agctctatca tggctgacag cgggtatggc tttgcagggt ggggatgggt aaggtgaaat
     2401 ctatcaatca gtaccggctt acgccgggct tcggcggttt tactccggta tcacatgtaa
     2461 ccacagcgtg ccgccttaca tgcgctgacg cgcataaaca acaagttgct acattagctt
     2521 acaggttaat tcaccttgca taagattagc atatagatta atgtttatcg aaatgggagg
     2581 tcatatgttt agcgttgttt atcacccgga agccagggaa gaagccacag cattacccat
     2641 aaaaatcagg gtgaagtttg accgtctcat cagcaaactg gaatatgacg cccgattact
     2701 gcgggaaccg gatacaaagc ccctggggga tggacttttt gagatccgca cgatggggac
     2761 ggacatagcc agaggtatct gggtatacca caaaggcaat accatcatca tgctccgggt
     2821 tttcatcaag aagtctcaga aaacgccagc aaaagaaatc gatctcgcca aaaagcggtt
     2881 agcggaggta cttaatgaaa ctggtaagcc ataaagaact gcataacgag tggatgcagg
     2941 acgacgaata tcgcgctgca tggcaggaag aagaacgtaa ggagaaactc agggagttgc
     3001 ttgcagaatg gcgtaagcat gacaatttga caaaagcgca ggttgctgaa cgcatggggg
     3061 ttactcctcc tgttatttcc cgcctggaaa acaacatcac taaagccagc atcgacaccc
     3121 tgactcgcta tgcccatgcg tgcggtatca aaaaacctgt tattacgctg tactgaactc
     3181 tgctctatgg ctgccagttc tggagaaatc agtaactaaa ttttaattag ctacgttatg
     3241 gtaaatctgc ctgatgtgtc gatttctctg aaagtaattc ggcttctgtg ggctgcgttt
     3301 gccttgtgcc acctgaatgt aatcactatg attaaatatg attaacaact aatcggatat
     3361 acaaatgaaa aacaataccg cacaggcaac aaaagtaatt accgcgcatg tgccattacc
     3421 tatggctgat aaagtcgacc agatggccgc cagactggaa cgctcccggg gctgggttat
     3481 caaacaggca ctttctgcat ggcttgccca ggaggaggag cgtaatcgcc tgacgctgga
     3541 agccctggac gatgtgatat ccggacagat tatcgaccat caggctgtac aggcctgggc
     3601 agacagcctc agtactgaca atccgttacc ggttccacgc tgatggaact gaagtggacc
     3661 agtaaggcgc tttctgattt ggcgcggtta tatgattttc tggtgctggc cagtaaacct
     3721 gccgccgcca gaatggtaca gtccctgata caggcaccgg tcattctgtt aactcatcca
     3781 cgtatgggag aacagttgtt tcagtttgaa cccagggagg tcagacggat ttttgctggc
     3841 gagtacgaaa tccgttacga acttaatggc cagactattt atgtattgcg cctgtggcac
     3901 acacgagaaa acaggtagat tgcgtgtggg gacaacttaa catagcctgc agatattaat
     3961 tcaggaacat tattatcagg ccagagattt tgcattccat taaata
//
LOCUS       DAZGAN020000228         3930 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGAN020000228 DAZGAN020000000
VERSION     DAZGAN020000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.229"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            38..913
                     /locus_tag="ACHKYO_RS24935"
                     /old_locus_tag="pgaptmp_004987"
     CDS             38..913
                     /locus_tag="ACHKYO_RS24935"
                     /old_locus_tag="pgaptmp_004987"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009384 - N-acylmannosamine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HGU4431651.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNELAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYIQKVSKHLSIYSEICNVMLFPAYFRSD
                     SGLIGATLWDRDCIT"
     gene            962..2434
                     /locus_tag="ACHKYO_RS24940"
                     /old_locus_tag="pgaptmp_004988"
     CDS             962..2434
                     /locus_tag="ACHKYO_RS24940"
                     /old_locus_tag="pgaptmp_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4431652.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            2438..3268
                     /locus_tag="ACHKYO_RS24945"
                     /old_locus_tag="pgaptmp_004989"
     CDS             2438..3268
                     /locus_tag="ACHKYO_RS24945"
                     /old_locus_tag="pgaptmp_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4431653.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDNSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            3314..>3930
                     /locus_tag="ACHKYO_RS24950"
                     /old_locus_tag="pgaptmp_004990"
     CDS             3314..>3930
                     /locus_tag="ACHKYO_RS24950"
                     /old_locus_tag="pgaptmp_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HGU4431654.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENELFALNYNKIDINKTYKIDDKWALIPGIQTQWMS
                     AGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKVN
                     DSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMSPVFTPY"
BASE COUNT         1089 a          628 c          955 g         1258 t
ORIGIN      
        1 tctgaataat tgcaagattg ttatataggt aaaatgaatg ataacactgg ctgttgatat
       61 aggaggaacg aaaatttcag cggcgttgat atctgatgac ggttcatttt tgctgaaaaa
      121 acagatatca acacctcatg aaagatgtcc tgacgaaatg acaggggctt tacgtctact
      181 ggtttctgaa atgaagggaa cagcggagcg atttgcagta gcatcaacag ggattattaa
      241 taatggagta ttgacagctt tgaatccaga caatcttgga gggctgaaag agtatcctct
      301 gaaaaatata atggaagata taactggttt aaatggaagt gtgataaatg atgcccaggc
      361 agccgcatgg gcagaatata cagttttgcc aaaagaaatt tgtgatatgg tttttataac
      421 cgtttctacg ggggttggag ggggaattgt agttaacaga aaattattaa caggtgtttc
      481 tggattagcc ggacatgttg ggcatatatt gtccggagta acggatacag aatgtggatg
      541 tggtcgtcgg ggatgcgttg aagcggtttc atctggtcgg gccatcatgg gggcagcaaa
      601 aaatgaactt gcaggatata gcaccaaata tatttttgaa ttagcgagac agggatataa
      661 ggaagccgaa tttttgacag agcgttctgc cagtactatt gctgaattaa tagtaagttt
      721 aaaattactg cttgattgtc aggttgttgt tgtcggagga agtgttggac ttgctgatgg
      781 gtatattcag aaagtgtcga aacatctttc catatattct gaaatttgta atgtaatgct
      841 gtttccggca tatttcagaa gtgactctgg tcttattggt gcaactcttt gggaccggga
      901 ttgtattact taaagtaata ctccagtgta ttataaatcc gctattattg tgaggtttaa
      961 tatggactcc tgtattcaga aaaagcaggt ttggtataag catctttcac ccagacagtg
     1021 gaagatattc ggtgcggcat ggacaggtta tttacttgat ggttttgatt tcgttctgat
     1081 atctctggtt cttacagagg ttcagcatga attcggcttg acgacaatag aagcggcaag
     1141 ccttatttcc gctgctttta tttcccgatg gtttgggggg cttgctattg gcgctttatc
     1201 ggacaagatg gggcgcagga tggcaatggt tttaagtata gtgctattct ctctcggaac
     1261 ccttgcctgt ggtctggctc ctggatatgc agtgatgttt attgctcgca ttgttattgg
     1321 gcttggtatg gcaggtgaat atggctcaag tgtaacatat gttatcgaaa gctggcctgt
     1381 tcatctcaga aacaaggcaa gtggtttttt aatttctgga ttttctattg ggggaggttt
     1441 agctgcacag gtttatagca ttgtagtacc actatggggc tggcgttctc ttttctttgt
     1501 cggcatgctt ccaatattat ttgcttttta tcttcgcaag aatcttccgg agtctgatga
     1561 ctggcaaaaa aggcaacaag agaataaacc cgtcagaaca atggtggata ttctgtacag
     1621 agaaaagaat aaatacataa atattcttct gtcttgtatt gcatttgcat gtctatatgt
     1681 ttgtttttca ggcgtcactg caaacgctgc tttaatcaca gttatggcat tgtgctgtgc
     1741 ggctgttttt atcagtttta tatatcaggg tatggggaaa agatggccaa caggcattat
     1801 gttgatgctt gtggttatgt tctgtttcct ctacggatgg ccactgcagg cttttcttcc
     1861 tacatggctt aaggttgata tgcagtattc accagaaact gttgctctca tatttatgct
     1921 ggcaggtttt ggctctgcag ctggaagttg tataggtggt tttatggggg actggcttgg
     1981 aacccgaaaa gcatatgtta tatcacttct tatcggtcag cttgttatta tccccgtttt
     2041 tcttgtggac cgggactatg tatggttact tggccttctg atctttactc agcaggtttt
     2101 tggtcagggg attggtgctc tggtgccgaa aataatcagt ggttatttta atgtggagca
     2161 gcgtgcggca ggactggggt ttatttataa tgtaggttca cttggtggag catgtgcgcc
     2221 tattcttggt gcagttgttg cgtctcatac aagtcttggt acagcaatgt gttctcttgc
     2281 ttttatctta acatttgttg ttcttgtcct tattggtttt gatatgccat cacgtgtaca
     2341 gagatggatc catccagaag ctgcgcttga atatgatact gtcgatggaa aaccttttta
     2401 tggagcaaga aaaaagaatg ttgcaggaga ataaaggatg ctatcaggga ataaaaaaat
     2461 gattcattac agggagaata tatattctct tgataattat gagcgttaca taactgacaa
     2521 ttcgctattt gagaatactg acgatggcat aatatataca ggtattgtgc cccttattac
     2581 agttgttgag cctgttgtat ctaatcaaca tagtgtgctg attatacctg gaggtggata
     2641 caggaggatt gtttatgaaa gagagggact tgatgtaggc agatggatag caggtcaggg
     2701 atatacggca tttattcttt cttacagaat gccctccgtt aatggttatt attcctttgc
     2761 ggatgcgaag aatgccctga actttatttc ttactgggcc ggaatgtcgg agagaacatt
     2821 taagagtgtt ggatgtatgg ggttttcggc aggtggtcat cttgctggat atattactca
     2881 tctccattat gaggaagaga cttctgtgcg tcctgactgg atggctctga tatatccagt
     2941 tatatctctt gacaggagta ttgctcatca gggatgccgg aaacgtcttc tggatgcagg
     3001 tgaaagtgaa tattcagcca gtcttgaaaa tagtgttaca gagtatatgc caccagtctt
     3061 tattgtgcat gcaacagatg ataaggatgt tccagttatt cactctctga gatattttaa
     3121 ctctatccgg gataagggac atcatgcaga agctcatttt tttgataagg gagggcatgg
     3181 atttggtgta tccggaaaaa atggatatcc gatagaaaaa tggcaaagcc tgcttctgga
     3241 atggcttgcg gaacataagt ttcattgaga aatcagatta caagtgataa atatatttaa
     3301 tgatgaggca tttatgaaaa aatattatcc actattaact gtgattcttc ttggtttacc
     3361 tttgtgctct caggcattaa ctcttaatct acgtggagga tacagaagcg ccagtcacgc
     3421 ttatgaaacg ttgattcagg ttagtcaggg gtggaataat ggttggtggg caagttttga
     3481 gtcaaatacc tggaatacaa ttcacaacaa tgaaaatgaa ttgtttgcac taaattataa
     3541 taaaatagat ataaataaaa catataaaat agatgacaaa tgggctctaa tcccagggat
     3601 tcagacacaa tggatgtcag caggaagtca gtataaccct tatctgaaaa tttcgtatca
     3661 ggcaacgcca gatcttaatc tggccttacg ctatcgctat gactggaaag ctttccgaca
     3721 gacggattta gatggcaaca gggcgcgtaa tgatcagcat cagtttgatg gttatgtgac
     3781 atacaaagtt aatgattcat ggttgtttgc atggcagacc acggtatata cgaaggttaa
     3841 taatttcaaa tatgggaatc ataaaaaaac agcaactgaa aatgcatttg ttctgcaata
     3901 taaaatgtca ccggtgttta ccccatatat
//
LOCUS       DAZGAN020000229         3783 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGAN020000229 DAZGAN020000000
VERSION     DAZGAN020000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.230"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..535
                     /locus_tag="ACHKYO_RS24955"
                     /old_locus_tag="pgaptmp_004991"
     CDS             <1..535
                     /locus_tag="ACHKYO_RS24955"
                     /old_locus_tag="pgaptmp_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4431655.1"
                     /translation="RQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAM
                     SFSPDSRLTIKALKMAWEIRSKPAGVMFHSDQGSHYTSRQFRQLLWRYQIKQSLSRRG
                     NCWDNSPMERFFRSLKNEWIPVTGYMNFSDAAHEITDYIVGYYNALRPHEYNGGLPPN
                     ESENRYWKNSKAVASFC"
     gene            948..2450
                     /locus_tag="ACHKYO_RS24960"
                     /old_locus_tag="pgaptmp_004992"
     CDS             948..2450
                     /locus_tag="ACHKYO_RS24960"
                     /old_locus_tag="pgaptmp_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431656.1"
                     /translation="MKKFYQFRDEQRKELEQHDFYSLISSDCIALKDKLLFAPVMAHF
                     IMNFRDMNKWVIRFDNNDNEYKSVINGGTIEDETHSRLFLEDWRKLYIDDKLNWKASD
                     VIYWLFISREMECFRKFGIDFMRLCVDDGGEPILRYSHSESGETCGNIFFSKISPIAD
                     QVANHLGISLRYFGTFHLNLENGHVWKSEGVFENIELSPDSYKKMATLSKRMFDIFEG
                     IHDSFYNYLSSYVLNGSHPSFFESLPVGKNVAPIYPEFVIENKSHNDGRHIEHINNYL
                     EKISSHEFFKWLINTSIDPQLKLKSFIPLWIVDIMGYRDINKYVFTYEQPESESEKII
                     NDYALHLSEHSRLFYHDWKSLQLDDMLRWSASDTLEFIFLNADMDMHRENIVKFSLFG
                     LKHRDPVIRFWFMMILELSGKEFFSHVGDIALQVESKYNIYLPYLCGRHATENEHEAY
                     NNMYEHFMVKEISHEQSDLIIQITDMVMRSLLNNLDISYRYVVNNLLAAR"
     gene            3236..3331
                     /gene="dqlB"
                     /locus_tag="ACHKYO_RS24965"
                     /old_locus_tag="pgaptmp_004993"
     CDS             3236..3331
                     /gene="dqlB"
                     /locus_tag="ACHKYO_RS24965"
                     /old_locus_tag="pgaptmp_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HGU4431657.1"
                     /translation="MINLLIIVLRAVVAVANALIAILELIRQFID"
BASE COUNT         1139 a          705 c          845 g         1094 t
ORIGIN      
        1 gcggcagttc gcagtgacag agccaaatca ggtatggtgc ggcgacgtga cgtacatctg
       61 gacggggaaa cgttgggcat accttgccgt tgttctcgac ctgtttgcaa ggaaaccggt
      121 aggttgggca atgtcgttct ctccggacag cagactgacc atcaaagcgc tgaaaatggc
      181 ctgggaaatc cgcagtaaac cagccggggt aatgttccac agcgatcagg gcagccacta
      241 tacaagcagg cagttccggc agttactgtg gcgttaccag atcaaacaga gtctgagtcg
      301 acgaggaaat tgctgggata acagcccgat ggagcgcttc ttcaggagtc tgaaaaacga
      361 gtggataccg gtgacgggtt acatgaactt cagcgatgct gcccatgaaa taacggacta
      421 tatcgttggg tattacaacg cgctcaggcc gcacgaatat aacggtgggt tgccaccaaa
      481 tgaatcggaa aaccgatact ggaaaaactc taaagcggtg gccagttttt gttgaccact
      541 acaggggaag atagtgcaac tcaccgatga tgaaaaaagt ggacgaagag acgataacta
      601 cactcccatc tagggatgct ttttttgtgg tactgtagtg tgggagtaag ttcgggtttc
      661 atactgaatg aggcgattgc gagctgcctt tcatcgaaaa ctaatgtcca ttgtcattgt
      721 attgtctgac tctatctcgt aacaagtctt tgacacaaag ttttatattt ttatcaaagt
      781 ggacatgagg tgaattaaat ttgtgatctt tttcaaataa tgtgctcttg atagtagatt
      841 ctggttagtg atatctatta acaatatatt aaatttcttt tgtattatcg ctgataaaac
      901 tactgtattg gtattctata tattattgag taacaggaga tatcactatg aagaaatttt
      961 atcaatttag ggatgaacag cgaaaggagc ttgaacaaca tgatttttat agtttgattt
     1021 cttcagattg tatagcgttg aaagacaaac tattatttgc tcctgttatg gctcatttca
     1081 taatgaactt cagagacatg aataaatggg ttatcagatt tgataacaat gataatgaat
     1141 ataaatctgt tataaacggt ggaacaatcg aggatgaaac acattcaaga ttgtttctgg
     1201 aagactggag gaagctatat atagatgaca aacttaactg gaaagcaagt gatgttatat
     1261 actggttgtt tattagtcga gaaatggagt gtttccgaaa atttggtatt gattttatga
     1321 ggctttgtgt agatgatgga ggagaaccaa tacttcgata ttctcactcc gagtcaggag
     1381 aaacttgcgg taatatattc ttttcaaaaa ttagtcctat tgctgatcaa gttgccaatc
     1441 atttgggaat atcactccgt tattttggaa catttcacct taatcttgaa aatggacatg
     1501 tatggaagtc agaaggtgtt tttgaaaata tagagttgtc accagattct tataagaaaa
     1561 tggctactct ctcaaagaga atgtttgaca tatttgaagg gattcatgac tctttttata
     1621 attacctgtc cagttatgtt cttaatggaa gtcatccgtc attttttgaa tcattacctg
     1681 tagggaaaaa tgttgcacct atataccctg agtttgtgat agaaaacaaa agccataacg
     1741 atggtagaca tattgaacat ataaacaatt acctggaaaa aatatcgagt catgagttct
     1801 ttaaatggct gattaacacc tcaatagacc ctcaattgaa attgaaaagt ttcatacctc
     1861 tttggattgt tgatattatg gggtatagag atattaataa atatgttttt acatatgaac
     1921 agcctgaatc agaaagtgaa aagattataa atgattatgc attacacctg tcagagcata
     1981 gccgtttatt ttatcatgac tggaagtcac ttcaacttga tgatatgtta cgttggagtg
     2041 ccagtgatac tcttgagttt atttttctta atgcagatat ggatatgcat agagaaaata
     2101 tagttaagtt ttctttgttc ggattaaaac acagagatcc tgttatcagg ttctggttta
     2161 tgatgatact ggagttaagt ggaaaagaat ttttctctca tgttggagat atagctttac
     2221 aggtggaaag taaatataat atttatctcc catatttatg tggacgccat gcaacagaaa
     2281 atgagcatga agcatacaat aatatgtatg agcattttat ggtaaaggaa attagccatg
     2341 aacaaagtga tctaataata caaattacag acatggttat gcggtcatta ttgaataatt
     2401 tggatatctc atatcgatat gtagtgaata atttattggc tgctcgttag aagaacagca
     2461 ttggtatgac tctgatgctc cttaaaatcc cacttttcag agtgggattt gtttaatgcc
     2521 cagaacaggg ggaatttcca ttcatgccca aaaaatattt tgatgccctc ccgccaattc
     2581 tcgcgttcag tccttcctga aaaacctcag aattcacgta gcgcatcgcc gctactgcat
     2641 ccattaaaaa actgacagtt tttttaatca tctcgtggac tcaacttact gaaaaaatgg
     2701 gatttatgaa aatgccaccg tcggcatcaa gcagttggtc agcgcaaccg gctgcatcga
     2761 ctgcaacaaa aaaactgaaa gagaaagccc ctcatatgag ggagaaaaaa aggaagcgca
     2821 gtcaacgaga ggcgtaaaaa aacccgactg gtgaggtcgg gttttaatac ggttccggtt
     2881 ctgagttggt ggctctgacc ggaggaagtc actctacacc taacgtttac attttatgca
     2941 ctcagccgca gtattgcaac taactgtggt taaggcacca catttagttg tgtatcgtgg
     3001 gttttgtata ccttataatc atgaagttag ccgtgtggtg tggcttcttc ctctgccctt
     3061 cccgtttatg caggtatgaa cgattttaaa gtcctggctg aacaaacagg tggtgctgtg
     3121 tttagtgctc tggcaggagg aagtcactct gcacctaaca atgcgagaga accgttgtga
     3181 caggcagtgt aagagcaagc aacggtgatt tcctccagtt ttactggaga aacggatgat
     3241 taatctgtta atcatcgttc tcagggctgt ggtcgccgtt gcaaacgcgc tgattgcaat
     3301 tctggaactg attcgccagt tcatcgactg atgacagcgg aaacgtaact aagggcagga
     3361 agtgaaccgc ttcctgccct ttaacgtact gtgacatttt tcaaaaaaac tcaccaatat
     3421 tctcctcggc agaaatgaca taactcactg taaaataaaa aatcatctaa acgctggctt
     3481 tgcatcgagc gcgttggcca acgcttttat cagctgcgac cgcaccagat aatttgaaca
     3541 agatttcccg cattacctgg acatacaggt tattttgtag gctgcgctcc tccaagccag
     3601 ttacctcggt tcaacgagcg ccaactccat aaacctttga aaaaccgccc accaaggcgg
     3661 ttttttcgtt cagccagacg acacatttca gggcagcagg cacaggtgat acagggcagg
     3721 cggtggaggc cgcctgtcag cccgcgcgaa aacccctggc aggctggcgc ctgccggaag
     3781 gga
//
LOCUS       DAZGAN020000230         3766 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGAN020000230 DAZGAN020000000
VERSION     DAZGAN020000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.231"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..881)
                     /locus_tag="ACHKYO_RS24970"
                     /old_locus_tag="pgaptmp_004994"
     CDS             complement(<1..881)
                     /locus_tag="ACHKYO_RS24970"
                     /old_locus_tag="pgaptmp_004994"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGU4431658.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSP
                     VKEKNLDVLSKLAERLL"
     gene            999..1551
                     /locus_tag="ACHKYO_RS24975"
                     /old_locus_tag="pgaptmp_004995"
                     /pseudo
     CDS             999..1551
                     /locus_tag="ACHKYO_RS24975"
                     /old_locus_tag="pgaptmp_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1785..2471)
                     /locus_tag="ACHKYO_RS24980"
                     /old_locus_tag="pgaptmp_004996"
     CDS             complement(1785..2471)
                     /locus_tag="ACHKYO_RS24980"
                     /old_locus_tag="pgaptmp_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGU4431659.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSIASMMAQMQMGDVKALFENKPFITLSEPACGAGSMILAMADILNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
     gene            complement(2583..3530)
                     /locus_tag="ACHKYO_RS24985"
                     /old_locus_tag="pgaptmp_004997"
     CDS             complement(2583..3530)
                     /locus_tag="ACHKYO_RS24985"
                     /old_locus_tag="pgaptmp_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGU4431660.1"
                     /translation="MSEWCKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRHGTGLSSPANQAFEQWLGLLVKNAVLDGETIKTI
                     ERLYHQSCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLAGLSEHIDAEACWSWLSDY
                     PEQAQPCDMLMVIPSRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGNNVRHARDE
                     MSSLVLLIDTPWYPPSGELVGEISKVFDCEVRHWYSEPVHGIQGYNCYDGGEHTDSDL
                     QAEWPGRETLPQPRLYLVEECAEEQTKAAPLPVPLVSAQ"
     gene            complement(3644..>3766)
                     /locus_tag="ACHKYO_RS24990"
                     /old_locus_tag="pgaptmp_004998"
     CDS             complement(3644..>3766)
                     /locus_tag="ACHKYO_RS24990"
                     /old_locus_tag="pgaptmp_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGU4431661.1"
                     /translation="IDGWLKVLKSDKKALFRACRQARKASEYLLTRRTSQECAA"
BASE COUNT         1026 a         1002 c          792 g          946 t
ORIGIN      
        1 tcgagcagtc gctctgcaag ttttgataat acatccagat tcttttcctt taccggagaa
       61 aaaggacttc tgcatacagg agtatttata atccccatat agtaaagaag agtttttata
      121 cctgccagga caccactctg aataagagta tcaataacct ggttacaggc cacctgcatt
      181 tccttcgcct taatcacatc attatttttg actgcttcaa atatttcaag ataacgccac
      241 cccattatat tatatgtact accgattccc ccatctgcac ctgcaattaa ccctgatgca
      301 aatatttcat cgtaaccatt atataacaca agctctggat gattcctttt gatttgttcc
      361 atctggaata aatcaccgga tgtctgtttt aatgcacaaa ccccgggtat tgtgacaagc
      421 tcatttatct gatctaaaga aaaacgcact ccgctaagag ccggtatatt ataaactacc
      481 atcggaagtc catctgcaga atcaataatc ttcctgtaat ggatacaatg ctcttcaaat
      541 gaaaaaggat aataaaatgg agtaacagca gatatagcat gatatccgta tttttttgca
      601 gcttttgcaa gtacctcgct ttctgccgta ctgattccgc ccacatgcgc tataagtgtt
      661 agtcttccat ctgattcatc ggctactgtt tcaagaattt tttctcgctc tgcaacattc
      721 tgaagaaatg cttctccagt agagcctcca acatacaatc ccttaatatt ttgttcgata
      781 ttaaaacgga tcagttttcg aagactctcc atatcgattt gctgggattg gtcataagga
      841 gtgggtaatg ctgatatgac ccctttaaat tcacattgca tttttaatca ccttcactta
      901 actgcataat gggataccta gtacaccatc aagtacaaag attatccaac agaaaagaag
      961 cacaatgtga agattatcaa aaaaaagcca tcagaaagct gtcatgccgc tcagttaagg
     1021 atcggttgat cgatccagtg tccagttact attctctgcg taaccctctg cgactatctt
     1081 gccctggaac tgattaccca cagccctgaa gagtccgaca ctgtcacgct acacaaacgg
     1141 cgtcttcctc tggaaatgat ggtctggtga attatcagta tggcccatga gcgtaaggaa
     1201 ccttcatcag attgtgaacc ggctggacat catgctgcca ggcagtagcg ccgtgctcca
     1261 tgcccgccag cggccaggaa gcggagccat acgctgcgtg ttcactcaaa cagcgcagct
     1321 ctagcataac accacgccat atccgctctg gtgcgatctg aatctgctga ccattgatgg
     1381 cgtgttctga cgaacacagg acacgcaaga acacgataaa gcttcactcg ccaaatacat
     1441 agtggaaacc agacacttta tcctcaggta aaaatggtct gtctgataga actgaccagc
     1501 catctactga cggcagcggc cttcgataca atgaatctgg cactaaataa gactggcaat
     1561 atagccgata atgcgttgaa tcagtggcta tatcgtatac ataaaacaga catcattatt
     1621 ctgtcttgtt gtccaacaca taaaaactaa taaaaacata ccattccctg tcgtcagaga
     1681 atttattgat aaacaggctt tattgaacaa atcaaacatt cagggaactg gcagttgaac
     1741 aaaacgccag tccgaagact ggcgcagtga taaaacattc tgcatcatgc cacattctgc
     1801 tcctgaagat gacgcagacg atggtcccag tttcccaggt aatgcccggg agtcagcagt
     1861 acgcggcgtc gctcattaca gagcgagtta ccaatcacca cttctccggc cacaccacac
     1921 aacgacagtt gtatatacgc catgccggct gccagcgggt caatatccgt tgccgacacc
     1981 cacatcctgt ggtatgccgg ataccccgaa cggttcagga tgtcagccat tgccagaatc
     2041 atactgccgg caccacaggc aggttcactg agcgtgataa agggtttgtt ctcaaacaac
     2101 gccttcacat ctcccatctg catctgtgcc atcatgctgg caatactcca gggcgtgaaa
     2161 aactgactcc ggtatttatc ccccagttcc agttgcatga acacactccc cagaaaatca
     2221 cagaatccct gctccagtcc ctcaatgaca tgagcaagca aatgcgcaat gtggataaca
     2281 tcctgctttt cataaccact gacgattcgc agatacttct gttcccgggc ctcactgaac
     2341 tgaagccggt tttccagggc aatcacacta cagctgacaa agtcttcaaa caccttatga
     2401 cgtttgtgat aacaggctgt ccggttaaac aacgaaatga actccttctg atggtcagtc
     2461 atggtggcca tgttttctcc gtaaaaacag ggaacacatg gcccggacag gggaacaggt
     2521 gttccccttc gggttgaaat gttgataaaa cgggcatcac ggacatgata cccggaggtt
     2581 aattactggg ctgagaccag aggaaccggt aaaggtgcgg ctttcgtctg ttcttcagcg
     2641 cactcctcca ccagatacag tcggggctgt ggaagtgttt ccctgcccgg ccattcggcc
     2701 tgcagatcac tgtccgtatg ctctccgcca tcgtaacagt tgtaaccctg tataccgtgt
     2761 accggctcgc tgtaccagtg tcggacctcg cagtcaaaca ccttcgagat ctcaccaacc
     2821 agttcgcctg aaggcggata ccagggcgtg tcgatcagca gcaccagact gctcatctca
     2881 tcacgcgcat ggcgaacatt atttcctgac ggccactcca caccgtatat ccggctgtaa
     2941 aggctggtcg ttgtggaaat accttgcagc agtccacctg aaccattcag ctctgtagcc
     3001 agacgggagg gtatgaccat cagcatgtca cagggctgcg cctgttccgg gtaatccgac
     3061 aaccagctcc agcaggcttc tgcatcaatg tgttcactca gcccggccag accaaaccag
     3121 tcagaatact gacggctcat cagcgtggtg atgatctccc tggcattatc cggcagatta
     3181 ccccagcgaa cagcgccgat acatgactgg tgataaagac gctcaatggt ctttatcgtt
     3241 tcaccatcca gaacggcatt ttttaccagc aaccccagcc actgctcaaa cgcctggtta
     3301 gccggagatg acaaccccgt tccgtgacga accagcgccg gatatggcgg atactgcgtc
     3361 gtccgtgtgg gttttaccag ccctgcacaa ccggccagaa acagctgtat actctgctgt
     3421 atggcatgac ggtagcgcgg ggtctcacgc ccttcaatcc actgtgtaag tacatcaaga
     3481 cagactgatt taccggtaat ttcaaaacgg tttttgcacc attcagacat tgtttacgct
     3541 cctctttttt atacagatgt aaaaagaggg cattgtcccg tgaggtacaa tgccctcacg
     3601 ggataaatgg cggtcaggtt ataaccgcca ccggtagtaa aaattatgct gcgcactcct
     3661 gcgaagttcg ccgcgtcagt aaatactctg atgctttacg tgcctgccgg caggcgcgaa
     3721 acagcgcttt tttgtcagac ttcagaacct taagccatcc gtcaat
//
LOCUS       DAZGAN020000231         3626 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGAN020000231 DAZGAN020000000
VERSION     DAZGAN020000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.233"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..193
                     /locus_tag="ACHKYO_RS24995"
                     /old_locus_tag="pgaptmp_004999"
                     /pseudo
     CDS             1..193
                     /locus_tag="ACHKYO_RS24995"
                     /old_locus_tag="pgaptmp_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            195..488
                     /locus_tag="ACHKYO_RS25000"
                     /old_locus_tag="pgaptmp_005000"
     CDS             195..488
                     /locus_tag="ACHKYO_RS25000"
                     /old_locus_tag="pgaptmp_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HGU4431662.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(549..1669)
                     /locus_tag="ACHKYO_RS25005"
                     /old_locus_tag="pgaptmp_005001"
                     /pseudo
     CDS             complement(549..1669)
                     /locus_tag="ACHKYO_RS25005"
                     /old_locus_tag="pgaptmp_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(1678..2445)
                     /gene="sctT"
                     /locus_tag="ACHKYO_RS25010"
                     /old_locus_tag="pgaptmp_005002"
     CDS             complement(1678..2445)
                     /gene="sctT"
                     /locus_tag="ACHKYO_RS25010"
                     /old_locus_tag="pgaptmp_005002"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HGU4431663.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWMFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLACYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(2447..2707)
                     /locus_tag="ACHKYO_RS25015"
                     /old_locus_tag="pgaptmp_005003"
     CDS             complement(2447..2707)
                     /locus_tag="ACHKYO_RS25015"
                     /old_locus_tag="pgaptmp_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4431664.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(2717..3382)
                     /locus_tag="ACHKYO_RS25020"
                     /old_locus_tag="pgaptmp_005004"
     CDS             complement(2717..3382)
                     /locus_tag="ACHKYO_RS25020"
                     /old_locus_tag="pgaptmp_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4431665.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(3372..>3626)
                     /locus_tag="ACHKYO_RS25025"
                     /old_locus_tag="pgaptmp_005005"
     CDS             complement(3372..>3626)
                     /locus_tag="ACHKYO_RS25025"
                     /old_locus_tag="pgaptmp_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HGU4431666.1"
                     /translation="DEQMINSFEELPVKNEFVLGKKIMNLYEIDDLCAKRIISLLPES
                     EKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGNNNVK"
BASE COUNT         1233 a          720 c          571 g         1102 t
ORIGIN      
        1 cctgcggtta tgtgtttctt actcgttaaa acaaatagcc attattaaag gaattgatta
       61 taaaacaata tcatatcaca aaataagagc tctaaataaa cttaacataa aaggtactgt
      121 ttagttgttt atagcactct gtgaatggga taaacattat tttaaactcc aatcttgcgt
      181 aagagaaagt tgatatgtca ttagtaggat ataatagtaa catcataata accatcgcgc
      241 tgatatcgtt gctctctgca tgcaccagag aaaattcatt aaaggagaat caaaataaaa
      301 gaagtggcga atcccaagcg atagtagatg caagacagtt ctttagcgaa cacccggaat
      361 atatatcatc agcagaactg gagcaaaagc tatatcgcga atttgcacag gttactacag
      421 ttccagctta taaagagatg agtatgtatc aattgcttat catttcacaa gatcgtttaa
      481 ctcattaaat aaggatctac acgctttttg cgtgtagatc cttataaatt tctgactacg
      541 acagatgttt aattttttaa attatcgtct ttattctcga tttttgtgtc ttcaccctca
      601 atgaatttat cttctgaaga gagctgttca tcttgaacag gttgcccagc attctccaca
      661 tcttcaaccc acagcacaag acgtaatatt tcatctatat tttcgaagct aacataatcg
      721 tagcgacgat gggtagcata attttttcgt gctaattttt tatctgtgat aattggtatt
      781 ccgatttcct ttgcatattt ccttacgcct aaggccacct cattagttac tcttacagaa
      841 atcaatggaa taggtgaaag atgtggctta aagtatatcc ctattgcaat gtgagtaggg
      901 ttggaaatca tcaaacgact attactgaca tcagacttca attgctcaga aagaatttcc
      961 tgatgcgctc tctgcgttta gacttaattt ctggatttcc ttcttgctcc ttgtattctc
     1021 ttttaacttc ttgtttatcc attttcatat ctttcataaa taaaaaatat tcagcgataa
     1081 aatcaaaaat taagacaatt atcatactgc cgagacaata cagtatgaga aggaatagca
     1141 tctctcccca atctgataat aaagatatga tatctccatc aagagtttta aaaagcagtg
     1201 atttatgatt actccaaaat actttgatcg ccaatgcaaa aaaaacaata taaagaattg
     1261 ctttaacaaa ttcttttacg gtttttaagc caaatatacg ctttaagcca tttactggat
     1321 tcaatgaatc aaacttaaat ttaacagctt cagttgctaa ttgcatttta ctttgaaacc
     1381 aggagacaag tattgttgtg aacatgcaaa ccagcaaaaa aggaatgttc gttttcaacc
     1441 aaccaacccc catagcttta aaatagtccc aaatatctgg tttagcgtta tgatcaagga
     1501 tgtattcaag aatcgacata atgtgatgca catcaaaaac atatccgaga tataaagtac
     1561 ccacaagcat tattacagag atggttaagt ctctactttt tagaatttgc ccttttttag
     1621 aagcatcctg gagtttcttt tgtgttggct tttcagtttt attggccatg aaacactcta
     1681 tttctcaatg tatagttgca attgttctgg gaaaaaggat aacggcatta ctcgttcagc
     1741 cagtattggg gcaaaataaa taattaatat taaaaatgcc agcgcacttt tgacagtcag
     1801 tgaaatggca aaagcattta gttgtgaagc ataacatgcc agcaatccca ggacagcctc
     1861 accgcccagc ataacggcaa tcacggggct tgcgtaaacg atagtatgag tcattatgtt
     1921 attaatatat gagaataaag gctctaattt aggaaaatta aaattgcctg aaggccataa
     1981 attataactt tgccaaagag tttccagaat aaagttcatt ccacctttag taagataaac
     2041 cgcagcagag aaaaggttaa ataatcttgc taattccgag gtatcgacac ctgtagccgg
     2101 atccaaagta ctacttaatg ttgcaccgcg ttggttatcg ataaagctac ctatagcaag
     2161 gaacatccaa aacggggatg ctaataagca tgcaataaac agccctatga taatttctct
     2221 ggcagcgaac aaagcaatat cgatttcatt taagttcaat aattcatagt ggtaatgctg
     2281 aactaatgca catgatacca ctattattat tgggtgacga actactgttg gtatattccc
     2341 actggcgaaa aatggtaaca aatagaaagt tggtgccaat cggcaaaaac cgagcgctgt
     2401 agcagccagc agactatgca actgatagag tatcgcctcc cccatgctat cctctcgcga
     2461 atgctaaatt cagcatttcg ataccaaatg aatataattt ttcaccatac cacccgctca
     2521 ttaaaaaaaa gcaaatactt acgcacaata atttcacgcc aaatggcaac gtctgttctt
     2581 gcaactgcgt taccgtttga aaaagcccta ccaataaacc aacaaatgtc gcaactgcaa
     2641 taggtcctgc agacataact aaaatcagat acagcgccct attccccgca aaaacaatat
     2701 cgtccatatt ttttcctcag ggattaatgg ataggtcaaa atattgtaaa atgagccctt
     2761 ttgataacat tgtccaaccg tccatcgcga caaacaaaat taattttatc ggtgtagata
     2821 tggttaccgg actcatcatc atcataccta acgttaacag aacgctggat ataaccagat
     2881 ccactacgac aaagggtaaa tagatataaa aacctataat aaatgcactt tttatctcac
     2941 tcaatgcata tgcgggcagt aacgagaaaa ttgaaatatt atcatcatcg attatctctt
     3001 cattgtcttc agaactattc accttctgaa tcttttcaaa aaagctaacc aactcaggtt
     3061 cagaatattt tatcaaatat gatttataac cactcatgcc tgtctcaaca aaattaacaa
     3121 ccgatgctac attattgaat gaaagattct cattctgact gttattatat atttccttcc
     3181 caaccggcat cattacgaac attgacaata gcaaagcaac cccattaagt gtcatatttg
     3241 atggcacttg ctgaagtccc agcgcgtttc ggacaataac aaaaacaatc gagaatttaa
     3301 taaaacatgt cccggatgct attataaacg gcaatagtgt aaataatgat aatatcgcta
     3361 tcagcgaaat gctatttgac attattattc cctgataacc aagaatgaat ttctacgccc
     3421 aatttatcat ccacttcaac aagttcgcca tagccagtta gcgcaccatt tacacgtatc
     3481 tctatatttt tctcagattc aggaagcaga gatattattc tttttgcaca gagatcgtct
     3541 atttcatata gattcattat tttcttacca agaacaaact cattttttac aggcaactct
     3601 tcaaaactat taatcatctg ttcgtc
//
LOCUS       DAZGAN020000232         3579 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGAN020000232 DAZGAN020000000
VERSION     DAZGAN020000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.234"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..179)
                     /locus_tag="ACHKYO_RS25030"
                     /old_locus_tag="pgaptmp_005006"
     CDS             complement(<1..179)
                     /locus_tag="ACHKYO_RS25030"
                     /old_locus_tag="pgaptmp_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4431667.1"
                     /translation="MGRPDWRAMLAGMSSTEYADWHRFYSTHYFHDVLLDMHFSGLTY
                     TVLSLFFSDPDMHPL"
     gene            complement(206..628)
                     /locus_tag="ACHKYO_RS25035"
                     /old_locus_tag="pgaptmp_005007"
     CDS             complement(206..628)
                     /locus_tag="ACHKYO_RS25035"
                     /old_locus_tag="pgaptmp_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4431668.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            complement(644..1384)
                     /locus_tag="ACHKYO_RS25040"
                     /old_locus_tag="pgaptmp_005008"
     CDS             complement(644..1384)
                     /locus_tag="ACHKYO_RS25040"
                     /old_locus_tag="pgaptmp_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431669.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            complement(1392..1787)
                     /locus_tag="ACHKYO_RS25045"
                     /old_locus_tag="pgaptmp_005009"
     CDS             complement(1392..1787)
                     /locus_tag="ACHKYO_RS25045"
                     /old_locus_tag="pgaptmp_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4431670.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(1784..2362)
                     /locus_tag="ACHKYO_RS25050"
                     /old_locus_tag="pgaptmp_005010"
     CDS             complement(1784..2362)
                     /locus_tag="ACHKYO_RS25050"
                     /old_locus_tag="pgaptmp_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431671.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(2374..2727)
                     /locus_tag="ACHKYO_RS25055"
                     /old_locus_tag="pgaptmp_005011"
     CDS             complement(2374..2727)
                     /locus_tag="ACHKYO_RS25055"
                     /old_locus_tag="pgaptmp_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4431672.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(2739..3134)
                     /locus_tag="ACHKYO_RS25060"
                     /old_locus_tag="pgaptmp_005012"
     CDS             complement(2739..3134)
                     /locus_tag="ACHKYO_RS25060"
                     /old_locus_tag="pgaptmp_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4431673.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            complement(3176..>3579)
                     /locus_tag="ACHKYO_RS25065"
                     /old_locus_tag="pgaptmp_005013"
     CDS             complement(3176..>3579)
                     /locus_tag="ACHKYO_RS25065"
                     /old_locus_tag="pgaptmp_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4431674.1"
                     /translation="RSFKAVKEKLDTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVV
                     YSGQYVENDVKKNFLPDNTMVLGNTQARGLRTYGCIQDADAQREGINASARYPKNWVT
                     TGDPAREFTMIQSAPLMLLADPDAFVSVQLA"
BASE COUNT          748 a         1120 c          907 g          804 t
ORIGIN      
        1 tccagcggat gcatatccgg atcgctgaaa aacaggctga gcacggtgta cgtcagcccg
       61 gaaaagtgca tatccagcag aacatcatga aaataatggg tactgtaaaa gcggtgccag
      121 tcggcatact ccgtggatga catcccggca agcatggcgc gccagtcggg tcgccccatc
      181 tcacgcgcca gtttcagggc aaaactcagc tcaccgtcga acactttccc gcagaaacag
      241 gctctgcagg cccggcgtcc tctgcctgtt caggggcatc attcaccaca aactcataca
      301 taccggacag ccggtacacc acgttttcag catgagaaat tgcctctgtg ggccaggtgg
      361 taagcacttc ctgctcaatc tgtttaacgg cttcattcat ggaaggctgc tttgtcttct
      421 gcggatggtt atgccacagg gacatagcca ccacaaaagc accggttctg atggcgtctt
      481 ccacagtaaa cttccggttg ctgtctgact ccgcctgttc tgcctgccgt ttcatcaggg
      541 cgagatgctc aatacgctgc agggctgaca gttcagaaag cgtgacggtc acgccgttat
      601 gttcaaatga ttcggttttc aggaacatcg ctgactctcc ggattaactg tcggtgacag
      661 tgatttctgc aaccgcagca agttcaccat taccggatac aaccggaatg ttgaccttac
      721 ctgcagcaac gcctttcacg gtgatggtca taccactgac cgacacggtg gcttttgttt
      781 tatccgcaga caccgcacga aagctcttgt cggttacgcc ctccggctgg aaggccacgg
      841 tcagcgtggt gctctgccct ttcaccaccg aggtgctggc aggcgtcacg gtcatgccgg
      901 ttgccgctgt taccgtgctg cgatcttctg ccatcgacgg acgtcccaca ttggtgactt
      961 tcaccgtgcg ggtgatcact tccttcgccg tcaccgcctt accgatactg ctgacccagc
     1021 cacggaacac atcgaccgtg ccgttcggga agcggatttt ataggcacgg gtatcgcctt
     1081 cattaaacca cgccagcagc gcctgctgcc cctgctctcc gggcatccac gccagcgtga
     1141 agctggtatc tccggcagat ttctgcccct gcccggtcgc agtccagtct gcatcttcat
     1201 catcgagata gctgtcgtca taggactcag cggtcagttc gccgggcgtc aggtctttaa
     1261 cttttgccag acgcgaccag tcaacgtctg aaagcggatt cgcataaggg tcaccgctcc
     1321 ccttataaac ccacagggtg gtcccggcac ctttcaccgg cattgtagga tttggtacag
     1381 gcatagcgtc ctcacatttc ataggtaatg acataagtca gatcggctga actccacagg
     1441 cccgcatcat cgtcgcgccg gtagtcatag ccgctggcca ccatactggt gatcaaatct
     1501 gacagtgccg ggatatcgct catcaccgga taaatccggg actccatcca cgcatccagc
     1561 tctgaatccg gcacctgagc aggcaggaaa acttcgatat gcagctccgc ctgccaggta
     1621 tcgctgtcca gctcttcgcc cgtgtattca gcgccggtga gataaacggc aactgccgga
     1681 aaatccgcct catcaaaaac agcggggcga ccatcaaaaa acgtcgcccc ggtgtcatgc
     1741 ttctccagtg catccagtac ggctgcacgg agttcagtat gtttcatcgc tttattacca
     1801 tcctcagttg atgctgcagc gcatagccca gctctttcgg aagacgttca cgccgtatcc
     1861 gctcaatatt ttgtttaaac gccgtggtca gcggcaccgc catcgggatt ttcaccacat
     1921 caatggggta acggtttttc ccggccacac gctgcatgac atgccaccgg ccatttttca
     1981 gttgctgaat aaacgcgccg ggaatacgac ggtttcccac cacaagcacg ctgccgccac
     2041 ctttcagggc tgaacgctgc ccctttttac gacgcctgcg tcgggacagg acaatccgcg
     2101 cgttacccag ctttattacg ggcaaatccc cccggttaac cttgattctg gcctgcggat
     2161 ttttgaccgt ggcccttttc agcctggccc tttcctttac cagtttccgg cgtacctttg
     2221 tctcacgggc aacctgtgac gccgactgcg atatcgcgga tgaagcaacg cggttaatgg
     2281 ccattgcggc ggcaccgggc accgccgttc tgctgatacg gctgaggttt tcaacggcct
     2341 gctcaagacc ttttatggcc atacatcccc ctttcagcgg cgacggttaa cggcaggcgg
     2401 tacgccccgc ccaagccaga gatgacagct tccgccatca tccggcgaaa cccggtccac
     2461 ccagaagttt tcctcgccga tggtcagtgt gtctccacgc cgcagctgcc gcacatcatc
     2521 agtccggaca aacagggacg ggctggagcc ttcaacgcgc accccctgtc cggcatagct
     2581 gatattttca gggtcatcaa aaacaccacg tatcacagca ccggacagct caccggatgt
     2641 catggtggct gacgttccca tgtacccgcg tatcgtttca tcggcgcggg caatggcagc
     2701 atcgaacagg ttatcggaat cagccacagc gcctcccgtt attgcattct ggccaggcca
     2761 tgttctgtca tttcggctgc cacaccggca gagacacgaa acgccgttcc cggcagcaca
     2821 aatgccacag gctcatcccg cgtggcgtga agtgcatcag tatgcagcgt caccagtgcc
     2881 acgaccgtga ccagagcagc cgtatcaatc acggtatccg gctgcgctga taccacctca
     2941 ttttcatgcc cggtcagcgc attttccggg ctgacagatg tgtcctgacc ggcagcgtca
     3001 tccgtgtcat caagctcctc ttccagctct gccacacgga gtgccagttc ttctttcgtc
     3061 cccgtcaggc tgacatcacg gttcagttgc tcacccagca cctgaagacg ggcaatcagt
     3121 tcatctttcg tcatggactc ctccacagag agaaaatggc cccgaagggc catgattacg
     3181 ccagttgtac ggacacgaac gcatcagggt cagccagcag catcagcggt gctgactgaa
     3241 tcatggtgaa ctcacgcgcc ggatcgccgg tggtcaccca gtttttcggg tagcgggcag
     3301 aggcgttaat accttcgcgc tgtgcgtccg catcctgaat gcagccatag gtgcgcagac
     3361 cgcgtgcctg agtgttcccc agcaccatcg tgttgtccgg aaggaagttc tttttgacgt
     3421 cgttttccac atactgtccg gaatacacga cgatcgccgt atcgccatac atccccttat
     3481 aggacaccgc ttcgcccagg tcttttaccg ctgtctccag ctcggaatta gagccgcgac
     3541 gggtatccag cttctccttg acggctttga aggaacgga
//
LOCUS       DAZGAN020000233         3575 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGAN020000233 DAZGAN020000000
VERSION     DAZGAN020000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.235"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            54..590
                     /gene="yfdR"
                     /locus_tag="ACHKYO_RS25070"
                     /old_locus_tag="pgaptmp_005014"
     CDS             54..590
                     /gene="yfdR"
                     /locus_tag="ACHKYO_RS25070"
                     /old_locus_tag="pgaptmp_005014"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4431675.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNDLSELRKCA"
     gene            581..943
                     /locus_tag="ACHKYO_RS25075"
                     /old_locus_tag="pgaptmp_005015"
     CDS             581..943
                     /locus_tag="ACHKYO_RS25075"
                     /old_locus_tag="pgaptmp_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431676.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLLRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            943..1248
                     /gene="yfdT"
                     /locus_tag="ACHKYO_RS25080"
                     /old_locus_tag="pgaptmp_005016"
     CDS             943..1248
                     /gene="yfdT"
                     /locus_tag="ACHKYO_RS25080"
                     /old_locus_tag="pgaptmp_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HGU4431677.1"
                     /translation="MTTFTNKELIKEIKERISSLEVRDDIERRAYEIALVSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            1164..1343
                     /locus_tag="ACHKYO_RS25085"
                     /old_locus_tag="pgaptmp_005017"
                     /pseudo
     CDS             1164..1343
                     /locus_tag="ACHKYO_RS25085"
                     /old_locus_tag="pgaptmp_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1475..2638
                     /gene="intD"
                     /locus_tag="ACHKYO_RS25090"
                     /old_locus_tag="pgaptmp_005018"
     CDS             1475..2638
                     /gene="intD"
                     /locus_tag="ACHKYO_RS25090"
                     /old_locus_tag="pgaptmp_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HGU4431678.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHCGIMEDIKKA"
     gene            complement(2654..2730)
                     /locus_tag="ACHKYO_RS25095"
                     /old_locus_tag="pgaptmp_005019"
     tRNA            complement(2654..2730)
                     /locus_tag="ACHKYO_RS25095"
                     /old_locus_tag="pgaptmp_005019"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2694..2696),aa:Arg,seq:tct)
     gene            2973..>3575
                     /gene="fimZ"
                     /locus_tag="ACHKYO_RS25100"
                     /old_locus_tag="pgaptmp_005020"
     CDS             2973..>3575
                     /gene="fimZ"
                     /locus_tag="ACHKYO_RS25100"
                     /old_locus_tag="pgaptmp_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HGU4431679.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELI"
BASE COUNT         1082 a          769 c          855 g          869 t
ORIGIN      
        1 gcttcctttt accagaattc agcgcggtgc agtgcatata cgtggagaac aaaatgtcat
       61 ttattaaaac tttttccggg aagcattttt attatgacaa gataaataaa gacgacatcg
      121 atattaacga tatcgcggtt tccctttcaa atatctgtcg ctttgccggt catctttcgc
      181 acttctacag cgtcgcccaa catgcggttc tttgcagcca gctggtgccg caggaatttg
      241 cttttgaagc gttaatgcat gatgcaacag aagcgtattg ccaggacatt cccgcaccac
      301 tgaaacgcct tcttcctgac tataaacgga tggaagaaaa aatagacgcc gtaatccgtg
      361 agaaatacgg gttaccccca gttatgagta cacccgtgaa atatgccgat ctcatcatgc
      421 tggcaaccga acgccgcgat ctcgggcttg atgatggctc tttctggcct gtactggaag
      481 gcatcccggc aacagagatg ttcaacgtga ttccactggc accgggtcat gcctacggga
      541 tgtttatgga acgttttaac gatttatcgg agttacgcaa atgcgcatga atgttttcga
      601 aatggaaggg tttcttcgcg ggaaatgtgt accgcgagat ctgaaagtga acgaaacaaa
      661 tgctgagtac ctgttacgta aattcgacgc gcttgaagct aaatgtgcgg cactggaaaa
      721 caaaataata ccagtgtcag ctgaactgcc accagcaaat gaaagtgttc tgttatttga
      781 tgctaatgga gaaggctggc tgattggctg gcgttctctc tggtacacct ggggacaaaa
      841 agaaaccgga gaatggcagt ggacatttca ggtcggggac cttgaaaacg tcaatatcac
      901 tcactgggca gtaatgccaa aagcaccgga ggctggagca taatgaccac atttaccaat
      961 aaagaactga ttaaagaaat caaagaacga atcagcagcc tagaggttcg agacgatatt
     1021 gagcgccgtg cttatgaaat cgcactcgta tctctggaag tagagccaga tgaacgcgaa
     1081 gcctatgaat tattcatgga aaagcgtttc ggtgacttag tagatcgtcg gagagcaaaa
     1141 aacggcgata acgaatacat ggcatgggat atgactctcg gttggatcgt ctggcagcaa
     1201 cgagctggta tccatttttc aacaatgtca cagcaagagg tgaaataatg gagccataca
     1261 gcctcacact cgatgaggcc tgtcattttc tcaaaatatc cagaccgact gccattaact
     1321 ggatacgcac aggccgccta caggcaacac gtaaagatcc aaccaagcca aaatctcctt
     1381 acctcacaac acggcaagct tgcattgcgg cgcttcagtc tccgctgcat actgtccagg
     1441 tgagcgcggg tgatggcata acagaggaaa gaaaatgtca ctcttccgca gaaatgaaat
     1501 atggtatgcc tcgtattcgc tcccgggcgg gaaacgaatt aaggaatctc ttggcacaaa
     1561 ggacaagcgg caagctcagg agttgcacga caagcgaaaa gcagaactct ggcgagtaga
     1621 aaagctaggg gatttacctg atgtcacttt tgaagaggcc tgcctaagat ggcttgagga
     1681 aaaagctgat aaaaaatctc tcgattcaga taaaagccgg attgagttct ggcttgaaca
     1741 ttttgagggt ataaggctta aagatatctc ggaggcaaag atttactctg ctgtaagcag
     1801 aatgcataac agaaagacga aagaaatatg gaaacagaaa gttcaggccg ccatcaggaa
     1861 aggtaaagaa ccgcctgttt atgaaccaaa gccagtatca actcagacaa aggcaaagca
     1921 tcttgccatg ataaaggcca ttctccgtgc tgcagaacgc gactggaagt ggctggaaaa
     1981 agcgcctgtc atcaagatac cagcggtcag aaacaagcga gtcagatggc tggaaaagga
     2041 ggaagcaaaa cgccttattg atgagtgccc cgaaccactg aaatctgtcg tcaagtttgc
     2101 gctggcaact ggtctgagaa agtcgaacat cataaatctg gaatggcaac aaatcgacat
     2161 gcagcgacga gttgcctggg tgaatccaga agagagcaaa tcaaaccgcg ccattggtgt
     2221 ggcgctgaac gataccgcct gtaaagtgtt gcgtgatcaa ataggcaagc atcacaaatg
     2281 ggtgtttgta cataccaagg cggctaagcg agcagatgga acatcaacgc ctgcggtcag
     2341 gaagatgcgc atcgacagca agacatcatg gctatcagct tgtcgtcgtg caggaattga
     2401 agatttccgt ttccatgacc tcagacacac ctgggcaagc tggctgatcc agtcaggcgt
     2461 cccattatct gtgcttcagg aaatgggcgg atgggagtcc atagaaatgg ttcgtaggta
     2521 tgctcacctt gcgcctaatc atttgacaga gcatgcaagg aaaatagacg acatttttgg
     2581 tgataatgtc ccaaatatgt cccactgtgg aattatggag gatataaaga aggcgtaact
     2641 gattgaattg taatggcgcg ccctgcagga ttcgaacctg cggcccacga cttagaaggt
     2701 cgttgctcta tccaactgag ctaagggcgc gttgataccg caatgcggtg taatcgcgtg
     2761 aattatacgg tcaacccttg ctgagtcaat ggcttttgat ctggttgctg aacaagtgaa
     2821 cgaccgcgtc tgattttctg atttatttcg ctatagcggc aaacaaacac acaccgctgc
     2881 gcgtctgaat caagaaaacc cgtattttca tgtatcaaag tacaatttac cgccctaacg
     2941 gaaaattgtc cgctcctatg agactggtaa ctatgaaacc aacgtcggtg atcattatgg
     3001 atactcatcc tatcatcaga atgtctattg aagttctgtt gcaaaaaaac agtgaattgc
     3061 agattgtcct gaaaacggat gattaccgca taaccatcga ttatctccga acccgtcctg
     3121 ttgatttaat cattatggat atagacttgc ccggaacaga cggttttacc ttcctgaaaa
     3181 ggatcaaaca aatccagagc acagtgaaag tgttattttt atcatcgaaa tcagaatgct
     3241 tttatgctgg cagagcgata caagctggtg ctaacggttt tgtcagtaaa tgcaatgatc
     3301 agaatgatat ttttcatgcc gttcagatga tcctctccgg atacacgttt tttcccagcg
     3361 aaacgcttaa ctatataaaa agcaataaat gtagtacgaa tagttcaacg gtcactgtgc
     3421 tatctaatcg tgaagtgacc atattacgtt atctggttag cggattatct aataaagaaa
     3481 ttgccgataa gttattactt agcaataaaa cagttagtgc gcataaatct aatatttatg
     3541 gcaagctagg tttgcattca attgtagagc ttatc
//
LOCUS       DAZGAN020000234         3559 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGAN020000234 DAZGAN020000000
VERSION     DAZGAN020000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.236"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            178..372
                     /locus_tag="ACHKYO_RS25105"
                     /old_locus_tag="pgaptmp_005021"
     CDS             178..372
                     /locus_tag="ACHKYO_RS25105"
                     /old_locus_tag="pgaptmp_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4431680.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
     gene            complement(537..743)
                     /gene="ybcW"
                     /locus_tag="ACHKYO_RS25110"
                     /old_locus_tag="pgaptmp_005022"
     CDS             complement(537..743)
                     /gene="ybcW"
                     /locus_tag="ACHKYO_RS25110"
                     /old_locus_tag="pgaptmp_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HGU4431681.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            1029..1439
                     /locus_tag="ACHKYO_RS25115"
                     /old_locus_tag="pgaptmp_005023"
     CDS             1029..1439
                     /locus_tag="ACHKYO_RS25115"
                     /old_locus_tag="pgaptmp_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HGU4431682.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSFDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYTFDNSLLFTESIQNTTQIFPR"
     gene            1730..2023
                     /gene="bor"
                     /locus_tag="ACHKYO_RS25120"
                     /old_locus_tag="pgaptmp_005024"
     CDS             1730..2023
                     /gene="bor"
                     /locus_tag="ACHKYO_RS25120"
                     /old_locus_tag="pgaptmp_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HGU4431683.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKQTAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSK"
     gene            complement(2114..2296)
                     /gene="rzoD"
                     /locus_tag="ACHKYO_RS25125"
                     /old_locus_tag="pgaptmp_005025"
     CDS             complement(2114..2296)
                     /gene="rzoD"
                     /locus_tag="ACHKYO_RS25125"
                     /old_locus_tag="pgaptmp_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HGU4431684.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPRPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(2513..3010)
                     /gene="rrrD"
                     /locus_tag="ACHKYO_RS25130"
                     /old_locus_tag="pgaptmp_005026"
     CDS             complement(2513..3010)
                     /gene="rrrD"
                     /locus_tag="ACHKYO_RS25130"
                     /old_locus_tag="pgaptmp_005026"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGU4431685.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(3010..3225)
                     /gene="essD"
                     /locus_tag="ACHKYO_RS25135"
                     /old_locus_tag="pgaptmp_005027"
     CDS             complement(3010..3225)
                     /gene="essD"
                     /locus_tag="ACHKYO_RS25135"
                     /old_locus_tag="pgaptmp_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HGU4431686.1"
                     /translation="MKFMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT         1069 a          814 c          697 g          979 t
ORIGIN      
        1 aaggacccgc aaaatgataa taattatcat ctacatgtca caacgtgcat ctacgccatc
       61 aaaccacgtc aaataatcaa ttatgacgca ggtatcgtat taattgatcc gcatcaactt
      121 aacgtaaaaa caacttcaga caatacaaat cagcgacact gaatacgggg caacctcatg
      181 tcaacgaaga acagaacccg cagaacaaca acccgcaaca tccgctttcc taaccaaatg
      241 attgaacaaa ttaacatcgc tcttgatcaa aaagggtccg ggaatttctc agcctgggtc
      301 attgaagcct gccgtcggag gctaacgtca gaaaagagag catatacatc aattaaaagt
      361 gatgaagaat gaacatcccg cgttcttccc tccgaacagg acgatattgt aaattcactt
      421 aattacgagg gcattgcagt aattgagttg cagttttacc actttcctga cagtgacaga
      481 ctgcgtgttg gctctgtcac aggttaagta gtttgaatga ttagcagtta tggtgatcag
      541 tcaaccacca gggaataatc cttcatatta ttatcgtgct tcaccaacgc tgcctcaatt
      601 gccctgaatg cttccagaga caccttatgt tctatacatg caattacaac atcagggtaa
      661 ctcatagaaa tggtgctatt aagcatattt tttacacgaa tcagatccac ggagggatca
      721 tcagcagatt gttctttatt cattttgtcg ctccatgcgc ttgctcttca tctagcggtt
      781 aaaatattac ttcaaatctt tctgtatgaa gatttgagca cgttggcctt acatacatct
      841 gtcggttgta tttccctcca gaatgccagc aggaccgcac tttgttacac aaccaatact
      901 attaattgaa aacattccta atatttgaca taaatcatca acaaaacaca aagaggtcag
      961 accagattga aacgataaaa acgataatgc aaactacgcg ccctcgtatc acatggaagg
     1021 ttttaccaat ggctcaggtt gccattttta aagaaatatt cgatcaagtg cgaaaagatt
     1081 taaactgtga attgttttat tctgaactaa aacgtcacaa tgtctcacat tatatttact
     1141 atctagccac agataatatt cacattgtgt tagaaaacga taacactgtg ttaataaaag
     1201 gacttaaaaa ggttgtaaat gttaaattct caagaaatac gcatcttata gaaacgtcct
     1261 ttgataggtt gaaatcaaga gaaatcacat ttcagcaata cagggaaaat cttgctaaag
     1321 caggagtttt ccgatgggtt acaaatatcc acgaacataa aagatattac tatacctttg
     1381 ataattcatt actatttact gagagcattc agaacactac acaaatcttt ccacgctaaa
     1441 tcataacgtc cggtttcttc cgtgtcagca ccggggcgtt ggcataatgc aatacgtgta
     1501 cgcgctaaac cctgtgtgca tcgtttttaa ttattcccgg acactcccgc agagaagttc
     1561 cccgtcaggg ctgtggacat agttaatccg ggaatacaat gacgattcat cgcacctgac
     1621 atacattaat aaatattaac aatatgaaat ttcaactcat tgtttagggt ttgtttaatt
     1681 ttctacacat acgattctgc gaacttcaaa aagcatcggg aataacacca tgaaaaaaat
     1741 gctactcgct actgcgctgg ccctgcttat tacaggatgt gctcaacaga cgtttactgt
     1801 tcaaaacaaa cagacagcag tagcaccaaa ggaaaccatc acccatcatt tcttcgtttc
     1861 tggaattggg cagaagaaaa ctgtcgatgc agccaaaatt tgtggcggcg cagaaaatgt
     1921 tgttaaaaca gaaacccagc aaacattcgt aaatggattg ctcggtttta ttactttagg
     1981 catttatact ccgctggaag cgcgtgtgta ttgctcaaaa taattgcatg agttgcccat
     2041 cgatatggtc agctctatct gcactgctca ttaatatact tctgggttcc ttccagttgt
     2101 ttttgcatag tgatcagcct ctctctgagg gtgaaataat cccgttcagc ggtgtctgcc
     2161 agtcgggggg aggctgcatt atccacgccg gaggccgtgg tggcttcacg cactgactga
     2221 cagactgctt tgatgtgcaa ccgacgacga ccagcggcaa catcatcacg cagagcatca
     2281 ttttcagctt tcgcatcagc taactccttc gtgtattttg catcgagcgc agcaacatca
     2341 cgctgacgca tctgcatgtc agtaattgcc gcgttcgcta gcttcagttc tctggcattt
     2401 ttgtcgcgct gggctttgta ggcgattgcg ttatcacggt aatgattgac cgcccatgac
     2461 aggctgacga tgatgcagat aatcagagcg gatataatcg cggttactct gctcactgtt
     2521 gcccccacaa acagacttca cgctcaatct cacgacgagt catcaggcct ttccattgct
     2581 taccgccagc gtatgtccag cgacgcagct gatcacatgc gcctttgata tcgccctggt
     2641 ttattttgcg aagaagcgtc gatgttctaa aattgccagc acccacattg taaacgaatg
     2701 agtaaagagc gccgcgcgtt gtttccggta tatcgacttt gatatacggg ttaatttgtc
     2761 tggcgacagt ggcaaggtct ttattcaaga gtgctttgca ttctgctttg gtatacgttt
     2821 taccgagcat gatgtctttt ccggtgtgtc cgtgacatac agtccataca ccaacaatat
     2881 ctttgtatgg tatgtagctg acaccttcca gaccatcgtt accacttggg ccagtgatta
     2941 acactgatgc tatagcaatt gctccgccac caatagcagc agcaacggct tttcgtaatg
     3001 atggaggcat tattcacctc tcgcagcctt gcgcttatct tctttaatct tgaaataaag
     3061 gtttgtcagg tacgtcagca ggccaaatac caggctaccc agcacaccta ttgctgccca
     3121 ctgtgaggga gtgactttat cgagcagctg taaaaaccag taaccggcac tacctgctga
     3181 ggtgccatag gcgactccag ttgttaactt atccatgaat ttcataaccc cacctcgcag
     3241 acaaagcggg tgtaaattga gggaatacaa cgtatcgcaa aaaagcagaa acgtaactga
     3301 ctccgagtca gtgaataact caggtattga gttatcagct aatatcgaga ctcaaaaaat
     3361 ggaaaaacca gctcgacggc gggtttaagc tgtgtgacga agtaaccact cttaacagca
     3421 taaccaattt tttacgtacg taaaccactg aatgatattt atgagaatgc taccgagtgt
     3481 tcaaaacatc accacaaata cataagaaaa cctcaataaa taaccaataa ataatttcca
     3541 gtgttatttt tagccggtt
//
LOCUS       DAZGAN020000235         3531 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGAN020000235 DAZGAN020000000
VERSION     DAZGAN020000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.237"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            55..561
                     /locus_tag="ACHKYO_RS25140"
                     /old_locus_tag="pgaptmp_005028"
     CDS             55..561
                     /locus_tag="ACHKYO_RS25140"
                     /old_locus_tag="pgaptmp_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4431687.1"
                     /translation="MILDQEWILASDLLGVGGLPSSLAGLHKRAKNEGWEKRSVTTPG
                     IRGRAFAYRLSDLPAHVVSIITGSAEIKHQPPQKNGGNSELLELIDTLSQEEQELVIY
                     TLRRKGVEKLLEFCNQENQELIALTGIRRLAALSLNNLSDQKVREIFEGDETKDHHFN
                     LTHKEAKA"
     gene            complement(886..1392)
                     /gene="tpx"
                     /locus_tag="ACHKYO_RS25145"
                     /old_locus_tag="pgaptmp_005029"
     CDS             complement(886..1392)
                     /gene="tpx"
                     /locus_tag="ACHKYO_RS25145"
                     /old_locus_tag="pgaptmp_005029"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGU4431688.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            1511..2476
                     /gene="ycjG"
                     /locus_tag="ACHKYO_RS25150"
                     /old_locus_tag="pgaptmp_005030"
     CDS             1511..2476
                     /gene="ycjG"
                     /locus_tag="ACHKYO_RS25150"
                     /old_locus_tag="pgaptmp_005030"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HGU4431689.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMVNSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(2451..3179)
                     /gene="mpaA"
                     /locus_tag="ACHKYO_RS25155"
                     /old_locus_tag="pgaptmp_005031"
     CDS             complement(2451..3179)
                     /gene="mpaA"
                     /locus_tag="ACHKYO_RS25155"
                     /old_locus_tag="pgaptmp_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HGU4431690.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
BASE COUNT          942 a          792 c          892 g          905 t
ORIGIN      
        1 gaaataaacc acataaaaga tataaacaaa agttaatatc aaatggagtt tgatatgatc
       61 cttgaccaag aatggatcct tgccagtgac ttgttggggg ttggaggtct tccttcatct
      121 ctggctgggc ttcataaacg agcaaaaaat gaaggatggg aaaaacgttc ggtaactaca
      181 cctggtatca gagggagagc atttgcctac cgtttaagcg acttacctgc acatgttgtt
      241 tccatcatta caggtagtgc agaaataaaa caccagcccc ctcaaaagaa cggtggcaat
      301 agcgagttgc tcgaactcat cgacactttg agccaagaag agcaagaatt agttatttac
      361 accttgagaa ggaaaggggt agaaaaattg ctggaattct gcaatcaaga gaatcaagag
      421 ttgatcgctt taacaggtat tcgacgcctg gcagcattga gccttaataa tttatcagat
      481 caaaaggtta gagagatttt cgagggagat gaaaccaagg atcatcattt caatttaacc
      541 cataaagaag ccaaagcgta accccatgaa gcccaggcta tgcacttagc gagcactttc
      601 aacttgattt gtgaccagat cgatattgat ataaactttt tagcataaaa gacattttaa
      661 gtacttttaa aaacgtccat aagcagtatg tgcagtatca aatttgttgc cgatacctta
      721 attttatacc ggattttatt taatcagtat caaaactaaa caacctccag aatcatcgat
      781 cacctgtttt atttcagtat cttacaggtg atttcacctc ctttcactaa aacccttgtc
      841 ggtatcaaat gatttacctg gttacacacc atcaggcata ttaaattatg ctttcagtac
      901 agccagagct gcttcgtaat ccggctcggt ggtgatttca tccaccagct ggctgaaaat
      961 cacattgtca ttttcgtcaa taaccacaac ggcacgcgct gccagacctt tcagtgggcc
     1021 atcagcaatt gccacaccgt aggcttgcag gaattcagcg ttacggaaag tggagagggt
     1081 gataacgttg ttcagacctt ctgcgccgca gaaacgagac tgggcgaacg gcagatcggc
     1141 agaaatacac agcacaacgg tgttgtcgat ctcggttgcc agttggttaa acttacgtac
     1201 tgatgcggcg caaactccgg tatcaatact cgggaaaatg ttcagcactt tgcgtttacc
     1261 cgcaaactga ccgagggtga cgtcagacag atcttttgct acgagagtaa aagtctgcgc
     1321 tttgctaccc gcctgcggga tggaattggc gactgtaacc gggttgccct ggaaatgaac
     1381 ggtttgtgac atgattatct ttcctgttta catatagtta acgtcacacc tagtttatgc
     1441 taactgtcaa taacacagca aacgctattt gcgcttaatc cgcagaccac cgcgacaaca
     1501 aggagtaaag atgagaaccg ttaaggtatt tgaggaagcc tggcccttac ataccccgtt
     1561 tgtgattgcc cggggaagtc gcagtgaagc gcgcgtggtg gtggttgaac tggaagaaga
     1621 gggtattaaa ggcaccggcg aatgcacgcc gtatccgcgt tatggagaaa gtgatgcctc
     1681 ggtaatggcg caaattatga gcgtcgtgtc gcaattagag aaagggctga cacgggagga
     1741 gttgcaaaaa attctccctg ccggcgcagc acgtaatgcg ctggattgtg cattgtggga
     1801 tctggccgcg cgaaaacagc agcaatcgct ggctgatttg atcggcataa cgcttcccga
     1861 gacagttatc actgcacaga cggtagtcat cggtacgcct gatcagatgg tcaatagtgc
     1921 atcaacactc tggcaggcag gcgcgaaatt actgaaagtg aagctggata accatcttat
     1981 tagtgagcgg atggtggcaa ttcgcacagc tgtgcccgat gcgacgctga tcgttgatgc
     2041 aaatgaatcc tggcgtgcag aagggttggc ggcgcgttgc cagctattgg cggatttagg
     2101 cgttgcgatg cttgaacaac cccttcctgc gcaggacgat gcggcgctgg agaattttat
     2161 tcatccgttg ccgatttgtg ctgatgagag ttgtcataca cgtagcaatc tgaaggcgct
     2221 gaaagggcgc tatgagatgg ttaacattaa gctcgataaa accggtggtc tgacggaagc
     2281 gctggcgctg gcgactgaag cgcgtgcaca aggtttcagt ctgatgctgg gctgcatgtt
     2341 gtgtacctct cgtgccatta gcgccgcttt accgctggtg ccgcaggtca gtttcgccga
     2401 tcttgacgga ccgacctggc tggcggtaga tgtggaaccg gcgcttcagt tcacgacggg
     2461 cgaattgcat ctttaggatg ccagcgcagc aagtttgcca tcgcaaagag atatttttcg
     2521 ctggcttcgt cggaggagat gggcggaaac tctgcggtga tgcaatgcaa gttgagatct
     2581 gcacaccaac tgccgaaaga gccaggcgtt tcataaccga cgctggttac cagaggcaat
     2641 tcaaacgcct gggccagcca ttcgcctaat tcgctgtgtc tgggatcttc aatacaggcc
     2701 agtggatcgt ggaatgagac cacccaggca ggttgaatgc ggtgtataag ctggcacagc
     2761 gcctgggttt caggttctga gccaggtttg tcacctgtca gtaatacgac atcacgttct
     2821 tcagcggcgc tgttccagcg ataaaccgtt tcaccttcct tccagttcgc cgccggaaag
     2881 tttctgttta aatccacacc attagcattg gcccgtaacc ccagctggca gccgtcagga
     2941 ttcacacaca gcaccacatg atggcggcgc aaagaaggtg tcaaagtccg cagagcacag
     3001 gagagcgtga cgaccgaaga gttttcatca ccgtgagtgc cagccagaat caaaccactt
     3061 tcgcgactgg ctgcaggggc aggaaaccag attaacggcg cgcccaataa tgaacgtccg
     3121 taatgttctg ttccgggcgg aaatgcgccg cgttcggcgc gtgggcggtt tacggtcatg
     3181 atcgtctctg gttcaggaag tattacaggc agtgttgtgc aaattttgcg gattatcaaa
     3241 tccttttctt caacgctatg tcatcgatct tgtatcactt ttttgccgga agttgtttgc
     3301 atttttattg atgtgcagat attagaatta tgctgactta gatgtaagtc gtaatgttat
     3361 atacatggcg tattttattc ttactggttc tacacatgtt aatcgcatcg gattgatcag
     3421 ttcaccttaa taagtttaat aaatcgtact gcacatagta cattacgatg gtaactatgt
     3481 aattgattta catgaataat ggcttgtcac catcagtcac gggttgattg a
//
LOCUS       DAZGAN020000236         3343 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZGAN020000236 DAZGAN020000000
VERSION     DAZGAN020000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.238"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..105
                     /gene="rrf"
                     /locus_tag="ACHKYO_RS25160"
                     /old_locus_tag="pgaptmp_005032"
     rRNA            <1..105
                     /gene="rrf"
                     /locus_tag="ACHKYO_RS25160"
                     /old_locus_tag="pgaptmp_005032"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(209..736)
                     /gene="mobB"
                     /locus_tag="ACHKYO_RS25165"
                     /old_locus_tag="pgaptmp_005033"
     CDS             complement(209..736)
                     /gene="mobB"
                     /locus_tag="ACHKYO_RS25165"
                     /old_locus_tag="pgaptmp_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HGU4431691.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(718..1302)
                     /gene="mobA"
                     /locus_tag="ACHKYO_RS25170"
                     /old_locus_tag="pgaptmp_005034"
     CDS             complement(718..1302)
                     /gene="mobA"
                     /locus_tag="ACHKYO_RS25170"
                     /old_locus_tag="pgaptmp_005034"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HGU4431692.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     AQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQVGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1372..1641
                     /locus_tag="ACHKYO_RS25175"
                     /old_locus_tag="pgaptmp_005035"
     CDS             1372..1641
                     /locus_tag="ACHKYO_RS25175"
                     /old_locus_tag="pgaptmp_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HGU4431693.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1718..2704
                     /gene="srkA"
                     /locus_tag="ACHKYO_RS25180"
                     /old_locus_tag="pgaptmp_005036"
     CDS             1718..2704
                     /gene="srkA"
                     /locus_tag="ACHKYO_RS25180"
                     /old_locus_tag="pgaptmp_005036"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HGU4431694.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQWMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2721..>3343
                     /gene="dsbA"
                     /locus_tag="ACHKYO_RS25185"
                     /old_locus_tag="pgaptmp_005037"
     CDS             2721..>3343
                     /gene="dsbA"
                     /locus_tag="ACHKYO_RS25185"
                     /old_locus_tag="pgaptmp_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HGU4431695.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEK"
BASE COUNT          842 a          810 c          824 g          867 t
ORIGIN      
        1 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
       61 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa attaagcaga
      121 aggccatccg tcaggatggc ctttttgcat tggcgcagaa aaaaatgccg gatgcgacgc
      181 tggcgcgtct tatccagcct actcttgctt atccgttctg cttttgcatc cactccacca
      241 caaaatcagc cagcccctca acatcattaa tatccagtaa tgcgacatca agattaagcg
      301 gcacatcact ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc
      361 cggctccatc gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca
      421 gaatcaaatc cagctttgag gtatccattc gacttgcgag aaactgtaga tccagctctt
      481 cttcgtctgg tgtttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgtg
      541 ccgcgccagc cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat
      601 ggtgcgtatg cttaatcagc cctggacgga tccctctggc gcataatgcc gggatcagtt
      661 gtttcaaaag cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca
      721 tcgtttttcc tgccatcggg caagctcctc tggcgtattc acgttaataa atgcatcttt
      781 attatcgctg aaatcaaccg catgaccgcc agccagacgc ataaatgcca ttacccggcg
      841 ttctcctact tgcagatatt ccagtaataa aggctcaatt gcgcggttta ccagagcaat
      901 agtcgggtga tcgcgttcac cgtcgtggac ccacacgaca ggcgcatctt tgcgctgatg
      961 attaagccgg gctgctaaat cctcgggaat ataaggagta tcgcacggac aaaacaaaaa
     1021 ccactcgcct gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc
     1081 cgccagtgaa tcttcaatca ctttcagacc gcttgcctga tagatttcct gatgacgatt
     1141 agcattaacc acgacgtgag agagctgcgc cataagcgcg tcagcgacat gttgccataa
     1201 tggtttgccg tttaattcca gcaatccttt atctacgccg cccattcgtc tggctttacc
     1261 gcctgccagc acaacgcctg ttatcgtcgt catcagattc accgatatcg cctcttttat
     1321 tgtgggattg accctgctaa cgtgtctgtc tcaagagtaa ggagcattac tatgaaatgt
     1381 aaacgtctga atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacctt
     1441 aacctgttgc aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg
     1501 gatttgacgg atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg
     1561 gtgatcccgg gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct
     1621 cgcggcgtaa ttaaagagta aaagcttgta agcggtgcca ccaaaatcat cgtgaaatga
     1681 tatccttcat cattcgtaat gttttccgga tgatgggatg aacaacagcg cttttacttt
     1741 ccagacacta cacccggata ccatcatgga tgctctattt gagcaaggga tccgggtgga
     1801 ttccggtctt accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga
     1861 tcgtcgacgt tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct
     1921 cgaagaacat caatttgcgt tgcagttggt aaatgatgaa gttccggtcg cagcacctgt
     1981 ggcctttaac ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag
     2041 cgtcggtggt cgccagttcg aagctgataa tatcgatcag atggaagcgg ttgggcgtta
     2101 tttagggcgt atgcaccaga cggggcgcaa acagcttttt atccatcgcc cgaccatcgg
     2161 tctgaatgaa tatctcattg agcctcgcaa gctgtttgag gacgctacac tgataccttc
     2221 cgggttgaaa gcggcattcc tgaaagcgac agatgagctg atcgccgccg ttacagcaca
     2281 ctggcgggaa gacttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct
     2341 ctggcgcgat ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagctattca
     2401 ggatttgtgg atgttgctca atggcgataa agccgagcag tggatgcaac tggaaactat
     2461 tattgaagct tatgaagaat ttagcgagtt cgacaccgct gaaatcggac tgattgaacc
     2521 tttacgcgcc atgcgtttgg tttattatct tgcctggcta atgcggcgtt gggctgatcc
     2581 cgcgttcccg aaaaattttc cgtggttaac cggggaagat tactggctgc gacagacggc
     2641 gacttttata gaacaggcaa aagttctaca agaaccccct ttgcaattaa cacctatgta
     2701 ttaatcggag agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag
     2761 cgtttagcgc atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac
     2821 cggtagctgg cgcgccgcaa gtgctggagt ttttctcttt cttctgcccg cactgctatc
     2881 agtttgaaga agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga
     2941 agatgactaa ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg
     3001 catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt gactgttccg ctgtttgaag
     3061 gcgtacagaa aacccagacc attcgttcag catctgatat ccgcgatgta tttatcaacg
     3121 caggtattaa aggtgaagag tacgacgcgg cgtggaacag cttcgtggtg aaatctctgg
     3181 tcgctcagca ggaaaaagct gcagctgacg tgcaattgcg tggtgttccg gcgatgtttg
     3241 ttaacggtaa atatcagctg aatccgcagg gcatggatac cagcaatatg gatgtttttg
     3301 ttcagcagta tgctgataca gtgaaatatc tgtccgagaa aaa
//
LOCUS       DAZGAN020000237         3255 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGAN020000237 DAZGAN020000000
VERSION     DAZGAN020000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.239"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..126
                     /locus_tag="ACHKYO_RS25190"
                     /old_locus_tag="pgaptmp_005038"
     CDS             <1..126
                     /locus_tag="ACHKYO_RS25190"
                     /old_locus_tag="pgaptmp_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431696.1"
                     /translation="VVMLAESRDFAELVAMKARALMKDMNMSSGVIKGNEFNQQG"
     gene            131..424
                     /locus_tag="ACHKYO_RS25195"
                     /old_locus_tag="pgaptmp_005039"
     CDS             131..424
                     /locus_tag="ACHKYO_RS25195"
                     /old_locus_tag="pgaptmp_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431697.1"
                     /translation="MSKVRVIFEFEHVSHDEKPAGNDSVEVHEKIGVDVKTERDTDNR
                     PTSLCDVYASILQYHSPVIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            436..966
                     /locus_tag="ACHKYO_RS25200"
                     /old_locus_tag="pgaptmp_005040"
     CDS             436..966
                     /locus_tag="ACHKYO_RS25200"
                     /old_locus_tag="pgaptmp_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4431698.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            1064..1606
                     /locus_tag="ACHKYO_RS25205"
                     /old_locus_tag="pgaptmp_005041"
     CDS             1064..1606
                     /locus_tag="ACHKYO_RS25205"
                     /old_locus_tag="pgaptmp_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431699.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGALPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIET
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNTDSWWTPSLSRDALCVVFKVGAYGREIKG
                     CIPEECQEIKHSEYVALTEE"
     gene            1610..2143
                     /locus_tag="ACHKYO_RS25210"
                     /old_locus_tag="pgaptmp_005042"
     CDS             1610..2143
                     /locus_tag="ACHKYO_RS25210"
                     /old_locus_tag="pgaptmp_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431700.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITEHVLRY
                     LIVDRTAAPAGISAEVA"
     gene            2143..2658
                     /locus_tag="ACHKYO_RS25215"
                     /old_locus_tag="pgaptmp_005043"
     CDS             2143..2658
                     /locus_tag="ACHKYO_RS25215"
                     /old_locus_tag="pgaptmp_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431701.1"
                     /translation="MFFKTSNPSALAAWQKYQQDYKKVKDEAKRLEAVLNVDCRSVFV
                     SGINGFYFKGLRFTDNKYPFHRDLWRKPTASNGWSCTPRTSCIPKALCAASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILFGPVEWFCVDDVIYLQCGVKPAKQKMTEILSD
                     EFYAAEKRVRG"
     gene            2662..3213
                     /locus_tag="ACHKYO_RS25220"
                     /old_locus_tag="pgaptmp_005044"
     CDS             2662..3213
                     /locus_tag="ACHKYO_RS25220"
                     /old_locus_tag="pgaptmp_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HGU4431702.1"
                     /translation="MMILQPMGRKGRAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHKRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAEDVVLRELMP"
BASE COUNT          862 a          683 c          885 g          825 t
ORIGIN      
        1 gtggttatgc tcgcggaaag cagagacttt gctgaacttg tggcaatgaa agccagagcg
       61 ctcatgaaag atatgaacat gagttccggg gttattaaag gtaatgaatt taatcagcag
      121 gggtaattcc atgagcaaag tacgcgttat ttttgaattt gagcatgttt cgcatgacga
      181 aaaaccggca ggcaatgaca gtgttgaagt gcatgaaaag attggagtgg atgtgaaaac
      241 agaacgtgat acggataaca ggccgacgtc actctgtgac gtttatgcaa gcattctcca
      301 gtatcacagt cctgtaatta ttcagtttct ctcagcggaa tttcaggcat ctgtacaggc
      361 ttttggggcg gatgccatca ttaaacgcca ccgcgtgcat aaagcatcag gcacactgca
      421 ataaggaaaa acaaaatggc taaacgtgtt acaaaattaa aggccgcagc cgaggctgca
      481 ccgcagaccc gcgaagaagt cagccgcgat atccgcaccc tgggcgatat tcagcgagag
      541 gcgctgcggc tggaaacggc gatgaatgat gaagtggcag aaatcaccgc ccgttatacg
      601 ccgcaaattg aaaaccttaa aaaagaaatc aaagtgcttt ttaaggggat tcaggactgg
      661 tgcaaaacca accgcgatga gctgacgaac ggcggcaaaa ccaaaacagc caatctgacc
      721 accggaacgg tgtcatggcg actggggaat ccatcatgca gcgtcagtcg tgatgtggaa
      781 ggtgtgattg aaatgctgcg ccgtatgggc cttgagcgtt ttatccgcac gaaagaggaa
      841 gtgaacaagg aagcggtcct ggcggagccg gatgcggtga aagggattgc cggtattaag
      901 gtgaataaag gcgctgaaag tttttatgtc gagccttttg aacaggacgc cggactgaat
      961 aaataacacc gcattaatca attaaatatc acttcgtttt aattatggcg ctcgcgtcag
     1021 gggactgcct gcgcctgtaa actgaaaata aggaacagga tatatggcat atttttattt
     1081 caaattagac cgtgtgcaga caaataaata tttcaccaaa tatcaacaga ctgttttacc
     1141 gctacgcaac agtattcttc gggcattact gaaaaataca ggcgctgccg gattgcgctt
     1201 aaagccgttt gccatggacg taatcagtga gttttatttt tctggtgctc tgcctgcggg
     1261 ctggcgtaag cgcgatgatg tggcttttat cggggacgga ccgtgcttta ttgccagacc
     1321 tgatgagtca tgccctgaag gtccggcgat tgccgcaatg attgaaaccg ctgaacgtga
     1381 gttaagaaag cgtcctgatt tccttgtctg gctctgtgaa aagctggggg taatgagaat
     1441 cccctccatg tttaacacgg actcctggtg gaccccgtcg ctctcccgtg atgccctgtg
     1501 cgtggtgttt aaagtaggcg cttatggcag ggaaataaaa gggtgtattc ctgaagaatg
     1561 ccaggaaatt aaacattctg aatatgtggc gctgacggag gaataattca tgattgatgc
     1621 aaaagtgctt gaaggggtta aaaactggct gagtatttac gggcgtctga cctgcggcat
     1681 tctggctgaa aaaatgaata tgccgccatc ctcgatggtt tattttctgc gtgatgcggt
     1741 cgatgccggg gtgctgacgg aatgtaacgg tttttatgat attccgcgtc cccgcccggt
     1801 gcagccggtt cgtcgcaaat gcagccagga aggtgcggct gatgatgttc agtggtgcag
     1861 cttcagaaaa tccctgccgt ggattgaggg gcatgatatt ccgtcgatgg cgtgggaatt
     1921 tgctcagggc gtactgacct gcgaaaccgt ttatgtggtg gctgaagttg atgagcaggc
     1981 catgaaagaa ggcgtgcccc agtttgtgat ggcgtatatc gacattcgcc tgggtgtcat
     2041 tatctgcggt ttaagcggct ggaatatcac cgaacatgtt ctgcgttacc tgattgttga
     2101 ccggacggct gcgcctgccg ggatatctgc ggaggtggcg taatgttctt taaaacatca
     2161 aacccttccg cgctggctgc gtggcaaaaa taccagcagg actacaagaa agttaaggat
     2221 gaggcaaaac gtcttgaggc cgtgctgaat gttgattgtc ggtcggtatt tgtatccggt
     2281 atcaatggtt tttattttaa aggactgcgt tttacggata acaaatatcc ttttcatcgc
     2341 gacttatggc gaaaaccgac tgcgtcgaat ggctggagct gcacgccgcg cacatcgtgt
     2401 attcccaaag ccctgtgcgc tgcctctgac gaacttaaca gtctgtggcg tgaatattcg
     2461 cccgtcacgt atgccagaac cgatgccctg ttgttctggc tgggtattga tttctcggca
     2521 atcctgtttg gccctgtgga gtggttctgc gttgacgatg tgatttacct tcagtgcggc
     2581 gtaaaacccg caaaacagaa aatgaccgaa attctgtctg atgagtttta tgctgctgaa
     2641 aagcgagtca gggggtgatg tatgatgatt ttacaaccca tggggcgaaa aggccgtgca
     2701 cccgctcatg tccgtgcgtg gacacctgaa gaagatgcac tgctgattgc gctttatcca
     2761 tccaccccgg ttaaggatat tgctgtcagg gtaaaaagaa gtttctgggg tgtacataac
     2821 cggattgttt tattacgcgg tacttacccg gagttgctca aatgcaaacg tccaagattt
     2881 aaacatgatg aagataaatt tatccgaaaa aatgccagaa cgatgaccgg taagcaaata
     2941 ggagaatatc tggggcgaga ccgggattct gtccacaaac gggcgcgata tattggcgta
     3001 agcatgaaaa aatacggaga gttgctgccc ttcacccgta tacctgatga tgatgttcat
     3061 cttattcgtg aattacggga tgctgaatca ccacgacgtc ttaccttccg ggaaataggc
     3121 gagaaatttg aattatccga gagtacggtg aattttattt atcaccatcg ccggactgcc
     3181 gaagatgttg tattacggga gttaatgcca tgataacgac attatttgtt gaatcagacg
     3241 agcctctggt atgtg
//
LOCUS       DAZGAN020000238         3255 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGAN020000238 DAZGAN020000000
VERSION     DAZGAN020000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.240"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..126
                     /locus_tag="ACHKYO_RS25225"
                     /old_locus_tag="pgaptmp_005045"
     CDS             <1..126
                     /locus_tag="ACHKYO_RS25225"
                     /old_locus_tag="pgaptmp_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431703.1"
                     /translation="VVMLAESRDFAELVAMKASALMKDMSMRSGVIKGNEFNQQG"
     gene            131..424
                     /locus_tag="ACHKYO_RS25230"
                     /old_locus_tag="pgaptmp_005046"
     CDS             131..424
                     /locus_tag="ACHKYO_RS25230"
                     /old_locus_tag="pgaptmp_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431704.1"
                     /translation="MSKVRVIFEFKHVSHDEKPAGNDCVEVHEKIGVDVKTERDTNNR
                     PTSLCDVYASILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            436..966
                     /locus_tag="ACHKYO_RS25235"
                     /old_locus_tag="pgaptmp_005047"
     CDS             436..966
                     /locus_tag="ACHKYO_RS25235"
                     /old_locus_tag="pgaptmp_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4431705.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            1064..1606
                     /locus_tag="ACHKYO_RS25240"
                     /old_locus_tag="pgaptmp_005048"
     CDS             1064..1606
                     /locus_tag="ACHKYO_RS25240"
                     /old_locus_tag="pgaptmp_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431706.1"
                     /translation="MAYFYFKLDIVYTNKYLTEYQRDVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGSLPAGWRKRDDVAFIGGGPCFIARPDESCPEGPAIAAMVEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNTDSWWTPSFSRDALCVVFKVGAYGREING
                     CIPEECQEIKHSEYVALTEE"
     gene            1610..2143
                     /locus_tag="ACHKYO_RS25245"
                     /old_locus_tag="pgaptmp_005049"
     CDS             1610..2143
                     /locus_tag="ACHKYO_RS25245"
                     /old_locus_tag="pgaptmp_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431707.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGVLAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQESAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGILTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITGHVLRY
                     LIVDRTAAPAAISAEVA"
     gene            2143..2658
                     /locus_tag="ACHKYO_RS25250"
                     /old_locus_tag="pgaptmp_005050"
     CDS             2143..2658
                     /locus_tag="ACHKYO_RS25250"
                     /old_locus_tag="pgaptmp_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431708.1"
                     /translation="MFFKTSNPAALAAWQKYQQDCQKVKDEAKRLEAVLNVDCRSVFV
                     SDISGFCFKGLSFTDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNILW
                     FEYSPVTYARTDALLFWLGIDFSAILHGPVKWFCVDDVIYLQCGVKPAKQRMTEILPD
                     EFYAAEKRVRG"
     gene            2662..3213
                     /locus_tag="ACHKYO_RS25255"
                     /old_locus_tag="pgaptmp_005051"
     CDS             2662..3213
                     /locus_tag="ACHKYO_RS25255"
                     /old_locus_tag="pgaptmp_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HGU4431709.1"
                     /translation="MMKLQPMGRKGRAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTHPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRISDDDVRLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAEDVVLRELMP"
BASE COUNT          865 a          683 c          889 g          818 t
ORIGIN      
        1 gtggttatgc tcgcggaaag cagagacttt gctgaacttg tggcaatgaa agccagtgcg
       61 ctcatgaaag atatgagcat gcgttccggg gttattaaag gtaatgaatt taatcagcag
      121 gggtaattcc atgagcaaag tacgcgttat ttttgaattt aagcatgttt cgcatgacga
      181 aaaaccggca ggcaatgact gtgttgaagt gcatgaaaag attggtgtgg atgtaaaaac
      241 agaacgtgat acgaataaca ggccgacgtc actctgtgac gtttatgcaa gtattctcca
      301 gtatcacagc cctgaaatta ttcagtttct ctcagcggaa tttcaggcat ctgtacaggc
      361 ttttggggcg gatgccatca ttaaacgcca ccgcgtgcat aaagcatcag gcacactgca
      421 ataaggaaaa acaaaatggc aaaacgcgtt acaaaattaa aggccgcagc agaggcggca
      481 ccgcagaccc gtgaagaggt cagccgcgat atccgcaccc tgggcgatat tcagcgagag
      541 gcgctgcggc tggaaacagc gatgaatgat gaagtggcag aaatcaccgc ccgttatacg
      601 ccgcagattg aaaaccttaa aaaagaaatc aaagtgcttt ttaaggggat tcaggactgg
      661 tgcaaaacca accgagatga gctgacgaac ggcggcaaaa caaaaactgc caatctgacc
      721 accggcacgg tgtcatggcg gctgggaaat ccatcatgca gcgtcagccg tgatgtggaa
      781 ggcgtgattg aaatgctgcg ccgtatgggg cttgagcgat tcatccgcac gaaagaggaa
      841 gtgaacaagg aagccgtcct ggcggagccg gatgcggtga aaggcattgc cggtataaag
      901 gtgaataaag gcgctgaaag tttttatgtc gagccttttg aacaggacgc cggactgaat
      961 aaataacacc acattaaatc tttaaatatc acatcgtttt aattatggcg ctcgcgtcag
     1021 gggacagcct gcgcctgaaa gctgaaaata aggaatcgga aacatggcat atttttattt
     1081 caaattagac atagtgtaca caaataaata tttaaccgaa tatcagcggg atgttttacc
     1141 gctacgcaac agtattcttc gggcattact gaaaaataca ggcgctgccg gattgcgctt
     1201 aaagccgttc gccatggacg taatcagtga gttttatttt tctggttctc tgcctgcggg
     1261 ctggcgtaag cgcgatgatg tggcttttat cgggggcgga ccgtgcttta ttgccagacc
     1321 tgatgagtca tgccctgaag gtccggcgat tgccgcaatg gttgaagccg ctgaacgcga
     1381 gttaagaaag cgtcctgatt tccttgtctg gctctgtgaa aagctggggg taatgagaat
     1441 cccctccatg tttaacacgg actcctggtg gaccccttcg ttctcccgtg atgccctgtg
     1501 cgtggtgttt aaagtgggcg cttatggcag ggaaataaac gggtgtattc ctgaagaatg
     1561 ccaggaaatt aaacattctg aatatgtggc gctgacggag gaataattca tgattgatgc
     1621 aaaagtgctt gaaggggtta aaaactggct gagtatttac gggcgtctga cctgcggagt
     1681 tctggctgaa aaaatgaata tgccgccatc ctcgatggtt tattttctgc gtgatgcggt
     1741 tgatgccggg gtgctgacgg aatgtaacgg tttttatgat attccgcgtc cccgcccggt
     1801 gcagccggtt cgtcgcaaat gcagccagga gtctgcggct gatgatgttc agtggtgcag
     1861 cttcagaaaa tccctgccgt ggattgaggg gcatgatatt ccgtcgatgg cgtgggaatt
     1921 tgctcagggc atactgacct gtgaaaccgt ttatgtggtg gctgaagttg atgagcaggc
     1981 catgaaagaa ggtgtacccc agtttgtgat ggcgtatatc gacatacgcc tgggtgtcat
     2041 tatctgcggt ttaagcggct ggaatatcac cggacatgtt ctgcgttacc tgattgttga
     2101 ccggacagcg gcaccggccg cgatttctgc ggaggtggcg taatgttctt taaaacatcg
     2161 aaccctgccg cgctggccgc gtggcaaaaa tatcagcagg actgccagaa agttaaggat
     2221 gaggcaaagc gccttgaggc tgtgctgaat gttgattgcc ggtcggtatt tgtatccgat
     2281 atcagtggct tttgttttaa ggggctgagt tttacggatg acaaatatcc ttttcatcgt
     2341 gatttatggc gaaaaccaac tgcgtcgaat ggctggagct gcacaccgcg cacgtcacgt
     2401 atccctaaag ccctgcgtgt tgcctctgat gaactgaata ttctgtggtt tgaatattcg
     2461 cccgtcacgt atgccagaac cgatgcgctg ttgttctggc tgggcattga cttctcagca
     2521 atattgcatg gtcccgtgaa gtggttctgc gttgacgatg tgatttatct ccagtgcggc
     2581 gtaaaacccg caaaacagag aatgaccgaa attctgcctg atgagtttta tgctgccgaa
     2641 aagcgagtca gggggtgatg catgatgaaa ttacaaccca tggggagaaa aggccgtgca
     2701 cccgctcatg tccgtgcgtg gacacctgaa gaagatgcac tgctgattgc gctttatcca
     2761 tccaccccgg ttaaggatat tgctgtcagg gtaaaaagaa gtttctgggg tgtacataac
     2821 cggattgttt tattacgcgg tactcacccg gagttgctca aatgcaaacg tccaagattt
     2881 aaacatgatg aagataaatt tatccgaaaa aatgccagaa caatgaccgg taagcaaata
     2941 ggagaatatc tggggcgaga ccgggattct gtccacaatc gggcgcgata tattggcgta
     3001 agcatgaaaa aatacggaga attgctgccc ttcacccgta tatctgatga tgatgttcgt
     3061 cttattcgtg aattacggga tgctgaatca ccacgacgtc ttaccttccg ggaaataggc
     3121 gagaaatttg aattatccga gagtacggta aattttattt atcaccatcg ccggactgcc
     3181 gaagatgttg tattacggga gttaatgcca tgataacgac attatttgtt gaatcagacg
     3241 agcctctggt atgtg
//
LOCUS       DAZGAN020000239         3191 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGAN020000239 DAZGAN020000000
VERSION     DAZGAN020000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.241"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            71..1207
                     /locus_tag="ACHKYO_RS25260"
                     /old_locus_tag="pgaptmp_005052"
     CDS             71..1207
                     /locus_tag="ACHKYO_RS25260"
                     /old_locus_tag="pgaptmp_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU4431710.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVGMMYQF"
     gene            1303..3000
                     /locus_tag="ACHKYO_RS25265"
                     /old_locus_tag="pgaptmp_005053"
     CDS             1303..3000
                     /locus_tag="ACHKYO_RS25265"
                     /old_locus_tag="pgaptmp_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HGU4431711.1"
                     /translation="MEHQLNKKLLNSSISLILASGLMMSTANAADDVKLKATKTNVAF
                     ADFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAI
                     EAAKKSTPTLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGI
                     PLEETFLSELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQ
                     NRGFNYFMGYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPF
                     MIYLAYSAPHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKL
                     VSSMDFYPTALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSH
                     WWDEENTAFWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLAL
                     YDINDLQQTKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
BASE COUNT         1005 a          619 c          681 g          886 t
ORIGIN      
        1 atagtgataa ttttttcgcc atagtttaat ttttaaaacc atactaaaaa tgtgcatagg
       61 ggattttttt atgcgaaaat taagtatggt tgtgtgctct gtcgtttctg cattaatgct
      121 gtgtgaggca acacaggcag cagaaatcta taataaagac ggtaataaac tggattttta
      181 tggtcgcgtt aaagcccgtc attacatctc tgatggttct atggatggcg acagaacatt
      241 tgcacgtctt ggtttcaaag gcgaaacaca aattaatgag agcctgactg gctttggtca
      301 gtgggaatac cagttcctgg ggtttaagac agaaagtgaa gtacgcaacg ataaaaatcg
      361 tctggcattt gcaggtattc gccacaatat actcggttca cttgattatg ggcgaaacta
      421 tggtgttgcc tatgatgtgg gggctctgac tgacgtactt ccggactttg gtggtgacag
      481 ctggacttac tctgataact atatgaccgg cagaacaacc ggagttctga cctggcgcaa
      541 tacagatttc ttcgggcttg ttgatggact tactttttct gctcaatatc agggtaaaaa
      601 tgaccgtgat aatttacttg aagctaatgg tgatggatat ggtttctccg taagctatga
      661 ctatgaaggt tttggaatcg ttgccgctta cactaatgcc gacagaacca gtctgcagga
      721 aaaagaaggc cgtaaacgtt tcatgacatc gaaagattca gaaggaaaag aagttattac
      781 gcctcgctgg attgccgggg gaaaacatgc ggagtttgca ggtgttgggg tcaagtataa
      841 tgataatgac ctgtatctgg cagctgacta ctctgaaacc cgaaatatga ctccatttgg
      901 tagcgcaggc atcgcagata aagcacggaa tattgaagtt gtggctcagt atatgtttga
      961 tttcggcctg cgtccttcac tggcttacgt tcagtccaaa gggatggatg ttaaaggtgg
     1021 aatagtaaac tatggcgatc agtctcttgt tgagtatatt gatgttggcg caaactactt
     1081 tctgaacaaa aacatgtcac ttcttattga ttacaaaatt aaccttattg atgaaagcga
     1141 atttaccaaa aaggctggag ttgcaaccga taatatactt gctgttggta tgatgtatca
     1201 gttttaacag gtatccattt aaacgattta catagttttt atttatatca gaggctttta
     1261 ttattaataa atgttcgtga tgcaactcag aaggaatacc atatggaaca tcagttaaat
     1321 aaaaaactgc ttaactcatc aatatcactt atattagcat ctggtctgat gatgagtact
     1381 gcaaatgcag cagatgatgt taagctcaaa gcaacaaaaa ccaatgttgc ctttgcggac
     1441 ttcacgccga cggaatacag cactaaaggt aagccaaata tcattgtttt aacaatggat
     1501 gatttagggt atggacaatt gccttttgat gatagttcat ttgaccctaa aacgatggat
     1561 aaccgaaatg ttgttgatac atacaaaata ggtattgata aagcaattga agctgcaaaa
     1621 aaatccactc caacattagc ggccctgatg gacgaaggtg taaaattcac taacggctat
     1681 gttgcacacg gtgtctctgg cccttcacgt gccgcaatcc tgacggcgcg agctcctgct
     1741 cgcttcggtg tatattcaaa ttctgatgca caggatggta tccctctgga agaaacattc
     1801 ctgtctgaat tacttcagaa tcatggttat tacactgcag caatcggtaa gtggcactta
     1861 tcaaaaatca gcaacgtagg tgtgcctgaa gaaaaacaaa cccgtgacta ccacgataac
     1921 ttcaccacct tctccgcaga agaatggcaa cctcagaatc gtggcttcaa ctacttcatg
     1981 ggttatcacg catccggcgt ttcactttat aactccccgt cattgttccg taaccgtgaa
     2041 aacattaaag ctgaagggta cattagtgat caattaactg atgaagcaat tggtgtaatt
     2101 gaccgtgcaa gcaaactgga acagccattt atgatttatc tggcatacag tgcaccacac
     2161 ctgcctaacg atcagcctgc gcctaaaaac tatcaggaga aattcaacac tggtagcaag
     2221 actgctgata actattatgc gtcaatttac tccgttgacc agggggttaa aaagatcctg
     2281 aatacactta aggaaaaagg tcagtacgac aacaccatca ttctgttcac ctctgataac
     2341 ggtgctgtaa ttgatggtcc actgccactg aatggtgcgc aggctggtta caaaggccaa
     2401 ccgtatccgg gtggtatgca tacacctatg ttcatgtggt ggaatggcaa gctgaaaacg
     2461 ggtgattatg acaaactggt ttcatcaatg gacttctatc cgaccgcttt agatgccgca
     2521 gatatcaaga tccctgcttc tctgaaacta gatggtgtat cattattgcc atatgtacag
     2581 aaaggagctg ataagaaatc tgcagctggc gaacctcaca aatatctgtc ctggattacc
     2641 tcttattctc actggtggga cgaagagaac actgcattct ggaacggata tcataaattc
     2701 gtgagaaaag agtctgatga ttatccgcac aacccacata cagaagactt aagccagttt
     2761 gcatacactg taagaagcaa cgattattct ctggtttaca cctatgcaga gaataaacta
     2821 gctctgtacg atatcaacga tctgcaacaa actaaagatc tctctgcatc ccatcctgat
     2881 gtagttaaga atatgaaggc tgttatgatt gacttcatca cgtcaagtca aaaaccgatc
     2941 agtgatatca accaagccaa atttgagaat ataaaaaaat ctttaactaa cgtgaaatga
     3001 taataagcag ggagtagata tatctactcc ctggatatag ggaaataaat cagttagagc
     3061 aataaataat gaacaaacac gaaaataaaa aatactgttc atctgacgag atttggtgta
     3121 cacaacgtaa ggagttaatc aaatctgttc ttgaaacaaa gaaagcttct gatttagaaa
     3181 aacagagtac a
//
LOCUS       DAZGAN020000240         3188 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGAN020000240 DAZGAN020000000
VERSION     DAZGAN020000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.242"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            354..1826
                     /gene="cta"
                     /locus_tag="ACHKYO_RS25270"
                     /old_locus_tag="pgaptmp_005054"
     CDS             354..1826
                     /gene="cta"
                     /locus_tag="ACHKYO_RS25270"
                     /old_locus_tag="pgaptmp_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin 10"
                     /protein_id="HGU4431712.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVG
                     GGGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQR
                     LKDIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALR
                     EAERQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELA
                     KAESDVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSV
                     TNALNSAVSIRDAKKTEVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEK
                     YSRIAQDLAEKAKGSKFNSVDEALAAFEKYKNVLDKKFSKVDRDDIFNALESITYDEW
                     AKHLEKISRALKVTGYLSFGHDVWDGTLKGLKTGDWKPLFVTLEKSAVDFGVAKIVAL
                     MFSFIVGAPLGFWGIAIITGIVSSYIGDDELNKLNELLGI"
     gene            complement(1823..2113)
                     /gene="cti"
                     /locus_tag="ACHKYO_RS25275"
                     /old_locus_tag="pgaptmp_005055"
     CDS             complement(1823..2113)
                     /gene="cti"
                     /locus_tag="ACHKYO_RS25275"
                     /old_locus_tag="pgaptmp_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 immunity protein"
                     /protein_id="HGU4431713.1"
                     /translation="MTVKYYLHNLLESLIPWLFYLLLNYKTPPFSLFIFIASIHVLLY
                     PYSKLTIFSFIQNTTKMKKEPWYSYNLFYFLYLAMAIPVGLPSFIYYSLKRN"
     gene            2138..2269
                     /gene="ctl"
                     /locus_tag="ACHKYO_RS25280"
                     /old_locus_tag="pgaptmp_005056"
     CDS             2138..2269
                     /gene="ctl"
                     /locus_tag="ACHKYO_RS25280"
                     /old_locus_tag="pgaptmp_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 lysis protein"
                     /protein_id="HGU4431714.1"
                     /translation="MKITFLIITILSLSGCQANYISDVQGGTVAPSSSSELTGIAVQ"
     gene            complement(2484..2625)
                     /locus_tag="ACHKYO_RS25285"
                     /old_locus_tag="pgaptmp_005057"
                     /pseudo
     CDS             complement(2484..2625)
                     /locus_tag="ACHKYO_RS25285"
                     /old_locus_tag="pgaptmp_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            2999..>3188
                     /locus_tag="ACHKYO_RS25290"
                     /old_locus_tag="pgaptmp_005058"
     CDS             2999..>3188
                     /locus_tag="ACHKYO_RS25290"
                     /old_locus_tag="pgaptmp_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431715.1"
                     /translation="MDNGVTAFEKGESARRSRTTERSESVSEEAEEHRKLHFLLTHLC
                     GISHPAWCTFHTIRTDSAW"
BASE COUNT          882 a          593 c          843 g          870 t
ORIGIN      
        1 tgtttctgga tgaggtcacg ggctttgtca tgctcatatc cggcagcaac aagggtgtcg
       61 agaagtcgtt tgtctgtttt gttcagttta tggcggggtg atttgctcac atcgttctcc
      121 gttttttcat gatccaacag ttttatatct catcctgatt tgctccggtg ttctgtttcg
      181 acgttctcaa tgcctgcctc cccatagtgt gatccttatt gcaaaaatga aaatccatgc
      241 tcttgacatg gacaatgctg agtagtaggt tttgctgtac ataaaaccag tggttatatg
      301 tacagtatgt tgtttttaat ttattgtttt aattatcaaa gaggaatttc tttatggata
      361 aagtcactga taattctcca gatgtggaga gcacagaatc tactgagggg tcattcccaa
      421 ctgttggggt tgatactggc gatacgatta cagcgacgct tgcaactgga actgaaaatg
      481 ttggtggagg cggtggagca tttggtgggg ccagtgaaag ttctgctgcg atacatgcaa
      541 ccgctaaatg gtctaccgcg cagttgaaaa aacatcaggc tgaacaggct gcccgtgctg
      601 ctgcggctga ggcagcattg gcaaaagcga aatctcagcg tgatgccctg actcaacgtc
      661 tcaaggatat tgttaatgac gctttacgtg ctaatgccgc ccgtagtcca tcagtaactg
      721 accttgctca tgccaataat atggcaatgc aggcagaggc tgagcgtttg cgccttgcga
      781 aggcagagca aaaagcccgt gaagaagctg aagcagcaga aaaagcgctc cgggaagcag
      841 aacgccaacg tgatgagatt gcccgccaac aggctgaaac cgcgcatttg ttagcaatgg
      901 cggaggcagc agaggctgag aaaaatcgac aggattctct tgatgaagag catcgggctg
      961 tggaagtggc agagaagaag ctggctgagg ctaaagctga actggcgaag gccgaaagcg
     1021 atgtacagag taagcaagcg attgtttcca gagttgcagg ggagcttgaa aacgctcaaa
     1081 aaagtgttga tgtgaaggtt accggatttc ctggatggcg tgatgttcag aaaaaactgg
     1141 agagacaatt gcaggataag aagaatgaat attcgtcagt gacgaatgct cttaattctg
     1201 ctgttagcat tagagatgct aaaaaaacag aagttcagaa tgctgagata aaattaaaag
     1261 aagctaagga tgctcttgag aagagtcagg taaaagactc tgttgatact atggttgggt
     1321 tttatcaata tataaccgaa caatatgggg aaaaatattc cagaatagct caggatttag
     1381 ctgaaaaggc gaagggtagt aaatttaata gtgttgatga agcacttgct gcatttgaaa
     1441 agtataaaaa tgtactggat aagaaattca gtaaggttga tagggatgat atttttaatg
     1501 ctttagagtc tattacttat gatgagtggg ccaagcatct agaaaagatc tctagagctc
     1561 ttaaggttac tggatatttg tctttcgggc atgatgtatg ggatggtacc ctaaagggat
     1621 taaaaacagg agactggaag cctttatttg tcactctgga gaagagcgcg gtagatttcg
     1681 gcgtggcaaa aattgtggca ttaatgttta gttttattgt tggtgcgcct cttggcttct
     1741 ggggaattgc aattatcaca ggtattgttt cttcttacat aggggatgat gagttgaaca
     1801 agcttaatga attgctaggt atttaatttc tctttagaga gtaatatatg aaacttggca
     1861 atcctactgg gattgccatt gctaggtaaa gaaaataaaa aaggttgtat gagtaccaag
     1921 gttctttttt catttttgtc gtgttttgta taaaagaaaa tatagttagt tttgaatatg
     1981 ggtatagtaa tacatgtata gatgcgatga atatgaaaag tgaaaatggg ggcgtcttat
     2041 agtttaataa caaatagaat agccatggga taagtgactc tagtaaattg tgtaggtagt
     2101 attttacggt cattatttat gttgttatag gggtagaatg aaaataacgt ttttgataat
     2161 tacaatatta tccttgtcgg gatgtcaagc aaattatatt agtgatgtac aaggagggac
     2221 ggtcgctcca tcttcctctt ctgaactgac agggatcgcg gttcagtaga aaagatcaaa
     2281 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2341 ccgctaccaa cggtggtttg tttgccggat caagatctac caactctttt tccgaaggta
     2401 actggcttca gcagagcgca gatacccaaa acttgtcctt ctagtgtagt caaaatcagg
     2461 ctgatacttc aagaactctg ttactaactt ataaatctcg ctctgctaac ctggttacca
     2521 atggctgctg ccagtggcgt taagtcgtgt cctttccggg ttggacccaa gagaacagtt
     2581 accggagaag gcgcagcagt cgggctgaac ggggggttcc tgcatacagc ccggcccgga
     2641 gcgaactgcc taagtcgccc cgagatacca gaagacgtga gctatgagaa agcgccacgt
     2701 gtcccgcaag ggagaaaggc ggaaaaggta tccggtaatc gatatggcgg gaacgtgggg
     2761 gcgcaggagg gagcgattca ccggaaacgg tgatgatctt taagccacgt cgggtttcgc
     2821 caccgctggc ggatttcatg gttgagccgt aaggctccca cagatgcacc gaaaaagtgt
     2881 ctgttttgtg aagtccgtca ggggggcgga gcctatggaa aaagcgcctg tgccgcggcc
     2941 tttcccgggg tcttcgtttt ttgcgcacat gttctttccc gggttatccc cggattctgt
     3001 ggataacggg gttaccgcct ttgagaaagg ggagtccgct cgccgcagtc gaacgaccga
     3061 gcgtagcgag tcagtgagcg aggaagcgga agagcaccgg aagctgcatt ttctccttac
     3121 gcatctgtgc ggcatttcac acccggcatg gtgcactttt catacaatcc gcactgattc
     3181 tgcatggt
//
LOCUS       DAZGAN020000241         3176 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGAN020000241 DAZGAN020000000
VERSION     DAZGAN020000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.243"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..188)
                     /locus_tag="ACHKYO_RS25295"
                     /old_locus_tag="pgaptmp_005059"
                     /pseudo
     CDS             complement(1..188)
                     /locus_tag="ACHKYO_RS25295"
                     /old_locus_tag="pgaptmp_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(318..971)
                     /locus_tag="ACHKYO_RS25300"
                     /old_locus_tag="pgaptmp_005060"
     CDS             complement(318..971)
                     /locus_tag="ACHKYO_RS25300"
                     /old_locus_tag="pgaptmp_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU4431716.1"
                     /translation="MMNMSDRIRQRRKELNLTQQALADLTGVNRVTVTGWEKDDYQPN
                     GANLQALANALKCDPLWLVSGKGSPEPKINLKPEIFAVKKVPLISWVQAGSWTMTEPG
                     VRKEDAEEWVYTTALVSEMAFALRVRGDSMTNPLGSPSIPEGSIVIVEPDIIDTECIN
                     GKIVVAHINGGQEATLKKFVEDWPNRYLVPLNPNYKTIECGENCRIVGLVKQVIMDF"
     gene            1067..1264
                     /locus_tag="ACHKYO_RS25305"
                     /old_locus_tag="pgaptmp_005061"
     CDS             1067..1264
                     /locus_tag="ACHKYO_RS25305"
                     /old_locus_tag="pgaptmp_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU4431717.1"
                     /translation="MRKSDVINYFGGVCKTAEALGIKHPSVSEWPEIIPEGRAYQLEK
                     ITNGKLKVDVSLYQKTNSAAA"
     gene            1292..1876
                     /locus_tag="ACHKYO_RS25310"
                     /old_locus_tag="pgaptmp_005062"
     CDS             1292..1876
                     /locus_tag="ACHKYO_RS25310"
                     /old_locus_tag="pgaptmp_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfL family putative regulatory protein"
                     /protein_id="HGU4431718.1"
                     /translation="MGKEPEWKVDKQPAWLVAAIRRTIADLPHGYEEAAEILGLYKSD
                     DITPAKDQLHNRLRSGGDQIFPLEWTMVLQDASGTRHVTDAIARRSNGVFVPLVDIDD
                     IDNGDINQRLMESIEWIGKHSQYLRKATADGVIDQAEREQIEENSYQVMAKWQEHLTL
                     LFRVFCAPENSNARECAAPGVVASIASGCGETNA"
     gene            2022..2201
                     /locus_tag="ACHKYO_RS25315"
                     /old_locus_tag="pgaptmp_005063"
     CDS             2022..2201
                     /locus_tag="ACHKYO_RS25315"
                     /old_locus_tag="pgaptmp_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4431719.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAY"
     gene            2131..3021
                     /locus_tag="ACHKYO_RS25320"
                     /old_locus_tag="pgaptmp_005064"
     CDS             2131..3021
                     /locus_tag="ACHKYO_RS25320"
                     /old_locus_tag="pgaptmp_005064"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4431720.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSAIPGRYTAQAPYKAGAGGR
                     SPELSTAIYDAPASFLSSALTHARIMVGWAGEPKGSPVSVDAGTANPVQSATSEFRSS
                     GGGSFPQSTEAAIMATIPTLTQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRTLE
                     CSASFTSRNFSVSDYTDCTGRKLPCYQITRDGFAFLAMGFTGKRAAQFKEAYINAFNQ
                     MEKQLSNPSVLSDVAHNASVLYSYISSIHQVWLQQLYPMLAKAESPLAVSLYDRINDA
                     ALLASLINLSLNPSEVRGRK"
BASE COUNT          812 a          741 c          792 g          831 t
ORIGIN      
        1 aaaaagagcg gtaccagccg gaagcaagtg tacaaactgg taccgccatg actacacaca
       61 gcataaagtt gtggtgccgg gtgcctcccg gtgcctggcg aaggttgcac accaggcggg
      121 tgggtatcca cagaaggtcg actgtcagcc tcaaccttaa cccgcgtgcg ctgagccgca
      181 ttcaccacaa ctctaaaaac aaatgtagga tatccaacat gtgagtgtca agagtttatg
      241 ttggttatcc tacataaaaa gataggctca taaaaaaacc ggggataccc cggttttgcg
      301 atagtgagga agatgtgtca aaaatccatt attacttgtt tgacaagacc aactattctg
      361 cagttctcac cgcattcaat agttttatag ttaggattta gtgggacgag atacctgttc
      421 ggccagtcct caacaaattt tttgagtgtc gcttcttgcc caccattgat atgggcaaca
      481 acgatttttc cgttaataca ctctgtatca ataatatctg gctctacgat aacgatagaa
      541 ccttctggta tcgatggtga gccgagggga ttggtcattg aatcaccacg gacccgtagt
      601 gcaaatgcca tttctgatac aagggcggta gtataaaccc actcttcagc atcttctttc
      661 ctgacaccag gctccgtcat tgtccatgaa cccgcctgaa cccacgagat gagggggact
      721 tttttaactg cgaatatttc aggttttaga tttatctttg gttcaggcga gccttttccg
      781 ctaacaagcc acagaggatc gcatttaagt gcgttggcta gggcttgaag gttggctcca
      841 tttggttggt agtcgtcctt ttcccatcca gtaaccgtga cacggttcac accagtcaaa
      901 tcagccagtg cttgttgtgt caggttcagt tcttttcgcc tttggcgaat acgatcactc
      961 atgttcatca tgtaggcaat cctaccacat gccaatgtag gattcttgac attggcatgt
     1021 tggatatcct acatttctgc ttaacgtaat ttaacgggag acagaaatgc ggaaatccga
     1081 cgtgattaat tatttcggcg gagtttgtaa aaccgccgaa gccctaggta ttaagcatcc
     1141 gtctgtttca gagtggcctg agattattcc tgaaggccga gcgtaccagt tagaaaaaat
     1201 tactaacggg aaactgaaag ttgacgtgtc tttatatcaa aagactaaca gtgctgcggc
     1261 ataaaaacac cacagaaatg aggaattaac cgtgggtaaa gaacctgaat ggaaagttga
     1321 taaacaacca gcatggctgg tggcagcaat acgaagaacg attgctgatt tacctcatgg
     1381 ctatgaggaa gcagcggaaa ttcttggttt gtataaatct gatgatatca ccccagcgaa
     1441 agatcaattg cataacagac tgcgtagcgg tggggatcaa atttttccac ttgagtggac
     1501 catggtttta caggatgcca gtggtaccag gcatgtaacg gatgcgatag cgcgtcgtag
     1561 taatggggtg tttgtgccgc tggtggacat tgatgacatt gacaatggtg acattaatca
     1621 gcggctgatg gagtcaatag aatggattgg caagcattcc cagtacttac gcaaggcaac
     1681 tgctgatgga gttattgacc aggctgagcg tgagcaaatc gaagagaaca gctaccaagt
     1741 aatggcgaag tggcaggagc atttaacact gttatttcgt gttttttgtg cgccggaaaa
     1801 tagtaacgcc cgcgagtgtg cagctccggg cgtcgtggcg tcgattgctt ctggttgtgg
     1861 agaaactaac gcatgaatag tttaacggca aataaccgtt tgtcgcaaca gctggtggtc
     1921 agcgtcgctg aacacctgtt gttacggcat gaatgcagat taccaaatca cctggctgta
     1981 agtaaccaca gagaacttta cctgactgtg gggggcgagt tgtgcaggaa cttaaccgct
     2041 ggtttcgtga cggaagaggg ctttatgtcc atgttattcg ttgggagcca gaaacacagc
     2101 gcgttatcta tcttcgcaaa gactacccgc atgagtgctt tagtcctttg tggaaattca
     2161 ggcgtgattt tgttgagtgt gaaggaccac cagcatattg attctgcaat tccgggacgt
     2221 tacactgctc aggcacctta taaagcgggt gccgggggtc gcagcccgga attgtcaacg
     2281 gcgatatatg acgcgccagc gtctttttta tcgtccgcgc tcacgcacgc cagaattatg
     2341 gtgggctggg caggggagcc gaaaggctcg ccggtctccg ttgacgccgg tactgcgaac
     2401 cctgttcagt ctgccaccag tgagtttcgc agctccggtg gtggaagttt tccacagtca
     2461 acggaggctg ccatcatggc tacgatccca accctcactc aacctgaaat tgccatcgtt
     2521 gatggtcagg ctgttacttc atccctggct gttgccaact tcttctccaa acgtcatgac
     2581 gatgtactga aaaagatccg cacgcttgaa tgctctgcat cattcacttc ccgcaatttt
     2641 tcggtgagtg attacaccga ttgcacaggc cgcaaactac cttgctatca aataacccgc
     2701 gacggctttg cgtttcttgc tatgggtttc acgggtaaac gtgctgccca gttcaaagag
     2761 gcatacatca atgcctttaa ccagatggag aaacagcttt caaatccctc tgtactgagc
     2821 gacgttgcac ataacgccag cgttctctat tcctacattt catcaattca tcaggtctgg
     2881 ctgcagcagc tttatcccat gttggcaaaa gccgaatccc cgctggctgt aagtctgtat
     2941 gaccgcatca acgacgcggc gctactggcc agtctcataa atttgtcgct gaacccttca
     3001 gaggtaaggg ggcgcaaatg atccggaata ttttcaaacg gtttaccaat cagactttcc
     3061 gttgtcctcg tcctggtcag tggtacacca cgcctgcagg gcatgttcta cgtgtcagcc
     3121 tggttgaccg tgaatgtcag aaggtgattt gtgaaccgct gggccgtaat taccgc
//
LOCUS       DAZGAN020000242         3164 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGAN020000242 DAZGAN020000000
VERSION     DAZGAN020000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.244"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..3109
                     /locus_tag="ACHKYO_RS25325"
                     /old_locus_tag="pgaptmp_005065"
                     /pseudo
     CDS             1..3109
                     /locus_tag="ACHKYO_RS25325"
                     /old_locus_tag="pgaptmp_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
BASE COUNT          853 a          670 c          916 g          725 t
ORIGIN      
        1 gcgagggggg gctggttacg gctgaaaata cgattatcgg tggcaatgcc accggtgtcg
       61 gaaccctgaa tgtgcaggat caagactctg tcatcacggt acgcagactc tataatggat
      121 atttcggtaa tggcgcagtc aatatttcca ataatggact aattaataac aaagaatatt
      181 cattggtggg cgttgaagac ggttcccacg gtgtcgtcaa cgtgaccgat aaagggcatt
      241 ggagtttcct cggaacaggc gaagctttcc gctatatcta tatcggtgat gctggtgacg
      301 gtgaacttaa tgtctcgcgt gaaggtaaag tagattcggg aattatcact gcggggatga
      361 aagaaacagg cacaggcaac attactgtta aggataagaa ctccgttatc actaatctcg
      421 gaactaatct tggttatgac ggccacggcg aaatgaatat cagtaatgag gggcttgttg
      481 tcagcaacgg aggaagctca ctcggttatg gagaaaacgg cgtcgggaat gtcagcatca
      541 ccacgggggg gatgtgggag gtcaataaga atgtctatac caccattggt gttgcgggcg
      601 tcggaaacct caatattagc gatggcggta agtttgtatc gcaaaatatt acttttttgg
      661 gcgataaagc aagcggtatc ggcacactga acctgatgga tgggacatca tcgttcgata
      721 ctgtgggtat caatgtcggt aattttggta gcggtatcgt aaatgtcagt aatggtgcca
      781 cccttaattc aacgggctat ggatttatcg gaggaaatgc ctccggtaaa gggatagtta
      841 atatttcaac ggatagtctc tggaatttaa agacatcatc tactaacgcc caattgctac
      901 aggtcggtgt attaggcacg ggtgaactga atattaccac cggaggtata gttaaagcgc
      961 gtgatacaca gatagctctc aatgacaaaa gtaagggcga cgtgagggtg gatgggcaga
     1021 actctcttct tgaaacattc aatatgtacg tagggacatc tggtacgggt acgttaaccc
     1081 tgacgaatag cggcacgctg aatgtcgaag gtggagaagt ttacttaggt gtttttgaac
     1141 ctgctgtagg aacgctaaac attggtgcag ctcacggtga ggcggcggca gatgccgggt
     1201 atataaccaa cgcgacgaaa gtagagtttg gttctggcga aggtgttttt gtctttaatc
     1261 atactaataa cagtgatgcc ggctaccagg tcgatatgct gattacgggt gacgataaag
     1321 acggaaaagt gatccatgat gcaggccata cggtgttcaa tgcagggaat acttatagcg
     1381 gtaaaacgct ggtcaatgac ggcctcctga ccattgcgtc tcatacggca gatggggtaa
     1441 cgggcatggg ttcgagtgaa gtcaccattg caagccccgg tacgctcgac attctcgcat
     1501 caacgaacag tgcaggagat tacacgctga ccaatgcgct caaaggcgat ggtttgatgc
     1561 gagtgcagct gtcatcctcc gacaagatgt ttggctttac ccatgcaaca gggactgaat
     1621 tcgccggtgt tgcccaactg aaagacagta ccttcactct ggaacgcgac aacaccgctg
     1681 cgcttactca cgcgatgttg cagtctgaca gtgaaaatac cacatcggta aaagtgggag
     1741 agcaatccat tggtggactg gccatgaatg gcggtacgct cattttcgat acggatattc
     1801 ctgctgcgac gcttgcagag ggatatatca gcgtcgatac gctggttgtc ggcgcgggtg
     1861 actacacctg gaaaggccgt aactatcagg taaacgggac gggcgacgtg cttatcgacg
     1921 tgcctaaacc gtggaatgat cccatggcga ataaccctct gacgacgctc aatttgctgg
     1981 aacacgacga tagccatgtc ggcgttcaac tggtgaaggc gcaaacggtt attgggtcgg
     2041 gtggctcatt aacgttacgt gatttacagg gcgacgaggt ggaagcggac aaaacgttac
     2101 acattgcgca aaacggaacg gtggtcgccg agggtgatta tggattccgc ctcacgaccg
     2161 caccaggtga tggtttgtac gttaactatg ggctgaaagc gctgaacatc catggtgggc
     2221 aaaagctgac gttagccgaa catggcggag cctatggcgc aacggccgat atgtcggcaa
     2281 aaatcggtgg tgaaggggat ctggcaatca atacggtgcg acaggtttcg ctttccaacg
     2341 gtcagaacga ctatcagggg gcaacctacg ttcagatggg gacattacgt accgatgcgg
     2401 atggtgcact tggcaacacc cgggaactga acatcagcaa cgcggccatc gtcgatctta
     2461 atggatctac gcagacggta gagacattca ccgggcagat gggttcgact gttttgttca
     2521 aagagggggc gctgacggta aataaaggtg ggatcagtca gggtgaactg acaggtggcg
     2581 gaaacctgaa tgttacaggg ggaacgctgg ctatcgaggg gcttaatgca cgctacaatg
     2641 cgttaaccag cattagccca aatgcggaag tcagcctcga taatactcag gggttaggca
     2701 gaggaaatat tgccaatgac ggtctgttaa cgctaaaaaa cgtgactggc gaactgcgta
     2761 atagcataag cgggaagggt atcgtgagcg caaccgccag gacagatgta gagctggatg
     2821 gcgataatag ccgctttgtg gggcaattca acattgatac aggcagcgcg ctcagcgtca
     2881 acgagcagaa aaacctgggt gatgcttccg ttatcaataa tggcctgctc accatctcca
     2941 ctgagcgtag ctgggcgatg acgcacagta tcagcggtag cggtgatgtg acaaaactgg
     3001 gtaccgggat cctgactctt aacaacgatt ccgcggcgta tcagggtacg acggatatcg
     3061 tgggggggaa attgctttcg gttccgactc tgccattaat atggcaagtt aacacattaa
     3121 tatccataac agcggtgtga tgtcgggaaa tgtcaccact gcag
//
LOCUS       DAZGAN020000243         3130 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGAN020000243 DAZGAN020000000
VERSION     DAZGAN020000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.245"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>3130
                     /locus_tag="ACHKYO_RS25330"
                     /old_locus_tag="pgaptmp_005066"
     CDS             <1..>3130
                     /locus_tag="ACHKYO_RS25330"
                     /old_locus_tag="pgaptmp_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD1 domain-containing protein"
                     /protein_id="HGU4431721.1"
                     /translation="VSSDGKITSGGDFMTEAQRVRRIFKDGRYVDAEYPVRQEVYDAF
                     KGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAARAFERYVQDKARMAGVENDYLV
                     NIRKAPEHNTDNTYAYPTNAELDGGIREAFDHLFRTLKTRETDKGVAFYSRKGVTRTP
                     EGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDSDLKVRVVKSQKEAEALAGELFD
                     GYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHAMEHVVTPAEYQTIIKTV
                     LKTRDSDNVTIREAWRKVDASYGKESPEVQAGEFLAHMAEKQPNKFVAAWERVVALVK
                     GVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDGSGASSQYSRSGKLDPFK
                     ADTYDAKQFASEVRRIAGLQSVPRGGEVRVGDTPPVLRALGAPNKELVMPAHVVHKAT
                     RPELREHYVSVETMADLPQLLADPVAVMTSKTEHDSLVTLIEAKDRRGAPVIVAIHMR
                     AKGAVAEVNKIASVYGKDNRIALQEQLDTSLLYLNKEKAADWLLSAGLQLPGANTING
                     SSGFKVLHPEDIRKGAYYSRTSSLTPEETIASRFVRQMQDKFQVLKAVQENIRKTGGK
                     IDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLANYKISQADLDEYLYARHAPE
                     RNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIVDDMLARRRELI
                     REAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGKGFTIGGRESRQAMGRASR
                     AQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDDRPDTMRTIAER
                     KDQETGETRREVVERPVPMAMMADRYFTTKKNGKTYYIKLHDPRLMRAMKNMGPETSN
                     AVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKLKGLDNLS
                     ALAVVKDSRSAMSAVYASLRGKTLTGNGAQWQKVWKEFVEDGGKTGWFNMGDLEGQQK
                     EMDRLVSLAKGGW"
BASE COUNT          804 a          741 c          932 g          653 t
ORIGIN      
        1 gtttccagtg acgggaaaat tacgtcaggt ggcgacttta tgacggaagc acagcgtgtc
       61 aggcgcatat ttaaagacgg caggtatgtt gatgctgaat atccggtacg tcaggaggtt
      121 tacgacgctt ttaaaggtgt gattcaggcc attaaaaaca gtgacatgcc gcgtcgttca
      181 gcgcttctcg atgaggtgcg ctcaaaaccg tactggtcaa cggatgttga aatggcggca
      241 cgtgcctttg agcgttatgt tcaggataag gcgcgtatgg ctggcgtgga gaatgattat
      301 ctggtcaata tccgtaaggc acctgagcac aacacagata acacctacgc ttatccgacg
      361 aatgcggaac tggatggcgg tattcgtgag gcattcgatc acctgttccg caccctgaaa
      421 acccgtgaga cggacaaggg cgttgcgttt tattcccgta agggcgttac ccgcacacct
      481 gaaggtaatc tcatttcgga tgttaaccgt agtgcggaag ccaaaggcag cccggtcccg
      541 caggttgaag cggttgcccg tggcgtgatg agcggcatta aggacagtga cctgaaggtc
      601 cgtgtggtga agtcacagaa agaggctgaa gcgctggcgg gtgaattgtt cgatggttac
      661 ggcagggtgc acgcattcta tcgtccggat aaacgagaaa ttgtcctggt ggcggataac
      721 attcctgacg ggcggaccgt tcgcgagaag ctgcgtcacg agatcattca ccatgccatg
      781 gagcatgttg tcacaccagc ggaatatcag acgattatca aaaccgtgct gaaaacccgc
      841 gacagtgata acgtcaccat ccgtgaagcc tggcgtaagg ttgatgcttc ctatggtaag
      901 gaatcaccgg aagtacaggc gggtgaattt ctggcacata tggcggagaa acagccgaat
      961 aaattcgtgg cggcatggga gcgtgttgtt gccctggtca aaggggtact gcgtcgtacg
     1021 gggttactga agccgacgga actgaacgat atcagacttg ttcgcgagac catccgtacg
     1081 ttaggccagc gtgtgcggga aggttacacg ccgcgtgagg atggctcggg cgcatcgtct
     1141 cagtactccc gtagcggtaa gctggatccg tttaaggctg atacctatga tgcaaagcag
     1201 tttgcttcag aagtgcgtcg cattgccgga ttacaatcgg ttccacgagg tggagaggtg
     1261 cgggttggtg acactccgcc agtgttaaga gcacttggcg caccaaataa agagctggtt
     1321 atgccagctc atgtggttca caaggccacc agaccagaat tacgtgagca ctatgtttct
     1381 gttgaaacga tggctgactt accgcaattg cttgccgacc cggttgcggt gatgacatca
     1441 aagacggaac atgactcact ggtaactttg attgaggcta aggacagaag aggtgcacct
     1501 gtcattgttg caattcacat gcgtgctaaa ggggcggtag ctgaggttaa taagattgcc
     1561 agtgtctacg gaaaggacaa caggatcgct ttgcaggaac agttggatac ttccttgctg
     1621 tatctgaata aagaaaaagc cgctgactgg cttctttcag ccgggctcca attgcccggg
     1681 gcgaacacca tcaacggctc ttctggattt aaagtactac atcctgaaga tattcgcaag
     1741 ggggcgtatt actcccgcac cagcagtctg acaccggaag agaccattgc atcgcgtttt
     1801 gttcgccaga tgcaggataa attccaggtg ctgaaggcgg tgcaggagaa tatccgtaaa
     1861 actggcggaa aaatagatga cagcaataac gcctatatgg ctgaagaact cttccacggg
     1921 aaggcggaaa acgacctgaa cgtaatgaag gagcgctacg ttcagccgct ggccaaattg
     1981 ctggcgaact acaaaatctc tcaggccgat ctggatgagt acctctacgc ccgtcacgcg
     2041 ccggaacgta acgcacatat tgcgaaaatc aaccctaaaa tgccggacgg cggttcgggg
     2101 atgaccaacg cggaggcggc ggaaatcatg cagcgtgtgc gtaacagcgg caaacaggca
     2161 cagtatgacc gtctggcagg gattgttgac gatatgctgg cccgtcgccg tgagcttatc
     2221 cgtgaggccg gacttgaaga gaacggtgtg gtggatgcct ggcagaacgc ctaccgttac
     2281 tacgttcccc tgaaaggtca ggatgttgac ggtgtggtgt cacttccccg tacaggtaag
     2341 ggcttcacca tcggcggacg tgaaagcagg caggccatgg ggcgtgcatc ccgtgcacag
     2401 tctccgtcca ctcaggcgat acaggacctg agcgaatcgc tgatccgcca tcgcaaaaac
     2461 gaagtgggta acgccttcct gaaactggtg caggataatc ccgacaagga ttactggcag
     2521 gtattcaccg atgacagacc ggataccatg cggacgattg cagagcgcaa ggaccaggaa
     2581 actggtgaaa cccgtcgcga agttgtcgaa cgccctgtac cgatggcaat gatggcagac
     2641 cggtacttca ccaccaaaaa gaacggcaaa acgtactaca tcaaactcca tgacccgcgc
     2701 ctgatgcgtg cgatgaagaa tatgggaccg gaaaccagta acgccgtaat ccgcacgctg
     2761 gggaaagtta accgcttcct ggcaacggtg aacacgtcgt ataacccgga attcctggtc
     2821 agtaacttca tccgtgacgt acagacggcg gtgatgaacc tgaaggcgga gcagggaagg
     2881 agcgacggta aactgaaagg gctggataac ttatccgcac tggctgtggt gaaagacagt
     2941 cggtctgcca tgtctgccgt atacgccagt ctgcgtggta aaaccctcac gggcaacggt
     3001 gcgcagtggc agaaggtgtg gaaagagttt gttgaggacg gaggtaaaac cggctggttt
     3061 aacatgggtg accttgaagg ccagcagaag gaaatggatc gccttgtctc actggcgaag
     3121 gggggatgga
//
LOCUS       DAZGAN020000244         3121 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGAN020000244 DAZGAN020000000
VERSION     DAZGAN020000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.246"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>3121
                     /locus_tag="ACHKYO_RS25335"
                     /old_locus_tag="pgaptmp_005067"
     CDS             <1..>3121
                     /locus_tag="ACHKYO_RS25335"
                     /old_locus_tag="pgaptmp_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD1 domain-containing protein"
                     /protein_id="HGU4431722.1"
                     /translation="VSTDGKITSGGDYMTEAQRAGRVFKDGRYVDAEYPVRQEVYDAF
                     KGVMKAINSSDMPRRSALLDEVRSKPYWSTDVEMAARAFERYVQDKARTAGVENDYLV
                     NIRKAPEHNTDNTWAYPTNAELDGGIREAFDHLFSTLKTRETDKGVAFYSRKGVTRTP
                     EGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDSDLKVRVVKSQKEAEALAGESFD
                     GYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHAMEHVVTPAEYQTIIKTV
                     LKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEKQPNKFVAAWERVVALVK
                     GVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDGAGASSQYSRSGKPDPFK
                     VPEGEGERYREWLGRAMRSKRSSDIQIELGRTPPVLRHLGAPDLPLVISRDTVRKATN
                     GVKHVVPMDVIERLPELMHDPEAVYQSATHQNSLVILFNLRDRNGDPVISAVHLKAIK
                     NGLEINKIASVYGTERGKKLSNMERQGLTLYRREKLTPDEDLPRGLQLPVVEDSLGSL
                     NKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQMQDKFQVLKAVQENIRKTGGKVDD
                     SNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLADYKIAQADLDEYLYARHAPERNA
                     HIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIVDDMLARRRELIREA
                     GLEESGVVDAWQNTYRYYVPLKGQDVDGVVSLPRTGKGFTIGGRESRQAMGRASRAQS
                     PSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDDRPDTMRVIAERKDQ
                     EAGETIREVVERPVAMAMMADRYFTTKKNGKTYYIKLHDPRLMRAMKNMGPETSNAVI
                     RTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKLKGLDNLSALA
                     VVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEFVEDGGKTGWFNMGDLEGQQKEMD
                     RLVSLAKGGW"
BASE COUNT          810 a          728 c          943 g          640 t
ORIGIN      
        1 gtttccactg acgggaagat cacgtcaggt ggcgactata tgacggaagc acaacgtgcc
       61 gggcgcgtat ttaaagacgg cagatatgtt gatgcggaat atccggtacg tcaggaggtt
      121 tacgacgcgt ttaaaggcgt gatgaaagcg atcaacagca gtgacatgcc gcgtcgttca
      181 gcgcttctcg atgaggtgcg ctcaaaaccg tactggtcaa cggatgttga aatggcggcg
      241 cgtgcctttg agcgttatgt tcaggataaa gcgcgtacgg ctggcgtgga gaatgattat
      301 ctggtcaata tccgtaaggc acctgagcac aacacagata acacctgggc ttatccgacg
      361 aatgcggaac tggatggcgg cattcgtgag gcattcgatc acctgttcag tacgctgaaa
      421 acccgtgaga cggacaaggg cgttgcgttt tattcccgta agggcgtgac ccgcacacct
      481 gaaggcaatc tcatttcgga tgttaaccgc agtgcggaag ccaaaggcag cccggtcccg
      541 caggttgaag cggttgcccg tggcgtgatg agtggcatta aggacagtga cctgaaggtc
      601 cgtgtggtga agtcacagaa agaggctgaa gcgctggcgg gggaatcatt cgacggttac
      661 ggcagggtgc acgcgttcta tcgtccggat aaacgtgaaa ttgtcctggt ggcggataac
      721 atccctgacg ggcggaccgt tcgcgagaag ttgcgtcacg agataattca ccatgctatg
      781 gagcatgttg tcacgccagc ggaatatcag acgattatta agaccgtgct gaaaacccgt
      841 gacagtgata acgccaccat ccgtgaagcc tggcgtaagg ttgatgcgtc ttatggtaag
      901 gaatcaccgg aagtgcaggc gggtgaattt ctggcacata tggcagagaa gcagcctaat
      961 aaatttgtgg cggcatggga gcgtgttgtt gccctggtca aaggggtact gcgtcgcacg
     1021 gggttactga agccgacgga actgaacgat atcagacttg ttcgcgagac catccgtacg
     1081 ttaggccagc gtgtgcggga aggttacacg ccgcgtgagg atggtgctgg cgcatcgtct
     1141 cagtactccc gtagcggtaa acctgatccg tttaaagtac cggagggtga gggtgagcgt
     1201 tatcgtgaat ggttgggaag agccatgcgc tccaaacgtt catctgatat tcagattgag
     1261 ctaggacgca cacctcctgt attgcgtcat cttggtgcgc cggacttgcc gctggttatt
     1321 tcccgcgaca ctgtgcgtaa ggccaccaat ggcgtgaaac atgtggtgcc aatggatgtt
     1381 atcgagagac taccggaact gatgcatgat ccagaagccg tttatcagtc cgcaactcat
     1441 caaaactctc ttgttattct gtttaatctc agagacagaa atggtgatcc agtaataagt
     1501 gcagtgcact taaaggccat taaaaatggg ctggagataa acaaaatagc ttctgtatat
     1561 ggaaccgaaa gagggaaaaa gttaagcaac atggagcgcc agggcttaac cctgtataga
     1621 agagaaaaat taaccccaga tgaggacctt ccccgggggc tccaattgcc cgtggtggaa
     1681 gactcactgg ggtctttgaa taaaatactc tatcctgaag atattcgcaa ggggccgtat
     1741 tactcccgca ccagcagcct gacgccggaa gagacaatcg catcgcgttt tgttcgccag
     1801 atgcaggata aattccaggt gctgaaggct gtgcaggaga atatccgtaa aacgggcggc
     1861 aaagtggacg acagtaacaa cgcttatatg gcggaagaac tcttccatgg gaaggcggaa
     1921 aacgacctga acgtgatgaa ggagcgctat gttcagccgc tggctaaatt actggcggac
     1981 tacaaaattg cgcaggccga tctggatgag tacctctacg cccgtcacgc gccggaacgt
     2041 aacgcgcata tcgcgaaaat caacccgaaa atgccggacg gcggttcggg gatgaccaac
     2101 gcggaagcgg cggaaatcat gcagcgtgtg cgtaacagtg gaaaacaggc acagtatgac
     2161 cgtctggcag ggattgttga cgatatgctg gcccgtcgcc gtgagcttat ccgtgaggcc
     2221 ggactggaag agagcggtgt ggtggatgcc tggcagaaca cctaccgtta ctacgttccc
     2281 ctgaaaggcc aggatgttga cggtgtggtg tcactgcccc gtacaggtaa gggcttcacc
     2341 atcggcggac gtgaaagcag gcaggccatg gggcgtgcat cccgtgcaca gtctccgtcc
     2401 actcaggcga tacaggacct gagcgaatcg ctgatccgcc atcgcaaaaa cgaagtgggt
     2461 aacgccttcc tgaaactggt gcaggataat cccgacaagg attactggca ggtattcact
     2521 gatgacagac cggataccat gcgtgtgatt gcagagcgca aggaccagga agctggtgaa
     2581 accattcgcg aagttgtcga gcgtccggtg gcgatggcaa tgatggcaga ccggtacttc
     2641 accaccaaaa agaacggcaa aacgtactac atcaaactcc atgatccgcg cctgatgcgt
     2701 gcgatgaaga atatgggacc ggaaaccagt aacgccgtaa tccgtacgct gggtaaagtt
     2761 aaccgcttcc tggcaacggt gaacacgtcg tataacccgg aattcctggt cagtaacttc
     2821 atccgtgacg tgcagacggc ggtgatgaac ctgaaggctg agcagggaag gagcgacggc
     2881 aaactgaaag ggctggataa cttatccgca ctggctgtgg tgaaagacag ccgttctgcc
     2941 atgtcagccg tatacgccag tctgcgtggc aaaaccctca cgggcaaagg tgcacagtgg
     3001 cagaaggtgt ggaaagagtt tgttgaggac ggaggtaaaa ccggctggtt taacatgggt
     3061 gaccttgaag gccagcagaa ggaaatggat cgccttgtat cgctggcgaa gggaggatgg
     3121 a
//
LOCUS       DAZGAN020000245         3109 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGAN020000245 DAZGAN020000000
VERSION     DAZGAN020000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.247"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(11..487)
                     /locus_tag="ACHKYO_RS25340"
                     /old_locus_tag="pgaptmp_005068"
     CDS             complement(11..487)
                     /locus_tag="ACHKYO_RS25340"
                     /old_locus_tag="pgaptmp_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HGU4431723.1"
                     /translation="MQIIIMTRDRYLEYGLMCMLSGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDCNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVVFHEYAFTFLADQLGISMKTVCAHLYNAMEKNGLRGVSIKYLCSTADR"
     gene            complement(550..756)
                     /locus_tag="ACHKYO_RS25345"
                     /old_locus_tag="pgaptmp_005069"
     CDS             complement(550..756)
                     /locus_tag="ACHKYO_RS25345"
                     /old_locus_tag="pgaptmp_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431724.1"
                     /translation="MLNYRISSVQVFTVNGRADRVQVRLECDDAAVFECCLLAEGEGE
                     LKELSLSELEERALMYAADSFRYE"
     gene            complement(884..2086)
                     /locus_tag="ACHKYO_RS25350"
                     /old_locus_tag="pgaptmp_005070"
     CDS             complement(884..2086)
                     /locus_tag="ACHKYO_RS25350"
                     /old_locus_tag="pgaptmp_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU4431725.1"
                     /translation="MKINRHGITLSLISFLIAGAPAFAQDDSSWASPGTASASETSST
                     AVGAWARAGKAGTALGSASYAHAPFSTSVGMSAYVNPKASNGVAVGSWAKADALNSVA
                     LGTGAQAMHRYSVALGQQSGTTRENEVYIGYDSGVTNPASPRVPDKTRVLGGVSDGTR
                     DTDAATVGQLNRKADEVYSDVSGRIAAEALKARDHTDTVAAENRENIIRNTVAINRNT
                     RGLLSQRDLLETHEERLNSQQQQINTGSTVAVDSHGYVTRGEGTGERITVQEGLVRTQ
                     EMATENRAAVSRNRQVGERNSRAIASQARTLQAHERRMNSQQRQINENHEEMKRAAAQ
                     SAALAGLFQPYSVGKFNATAAVGGYSDRQAVAVGMGYRFNEQAAAKAGTAFSDGDVSW
                     NVGVNWEF"
     gene            complement(2354..>3109)
                     /locus_tag="ACHKYO_RS25355"
                     /old_locus_tag="pgaptmp_005071"
     CDS             complement(2354..>3109)
                     /locus_tag="ACHKYO_RS25355"
                     /old_locus_tag="pgaptmp_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431726.1"
                     /translation="EPGKGANRVDGGSGYDTLLLSDVMDDWNIYRVSDGTLFFHSERG
                     AGLTEATGIEGISFSGDWRSKLSPYQIEEEQITDHRYLLKWRNKNVDYGKHTEGTNGD
                     DSLFGNIIFGLDGNDILHSAGGEGVSLLHGGRGNDYLHGASGNDELYGAEGNDWLYGG
                     GGNDYLYGGTGHDVFVFDAACEGNSFIMDFNKHKGEKDFLFLSKDIVTSTDEFISCLK
                     QTGNDVEAGIAGGRIYFVNTALEDINHNSVYIV"
BASE COUNT          834 a          917 c          598 g          760 t
ORIGIN      
        1 tatcccggat ttaccggtct gcggtgctgc agagatattt aatactgacg ccgcgcagac
       61 catttttctc catcgcattg taaagatgcg cacataccgt cttcatgctt atccccagct
      121 ggtctgccag aaaggtaaaa gcgtactcat ggaaaacaac tccgaagacc accaccatct
      181 cggtacgggt cagtggtctt gccgtatgtc cgaacagcca cgccccacga cggatggcct
      241 gcctgatatc tgtcaggcat ctcacagagg aaacaggaat gacaagaaaa cgacgcccac
      301 agaacataca gtatgtaagc ctttccagat tacagtcaca gagaatcaca taactgtctt
      361 cgggaagtgg cagacggcgc tttccggcat caaacagttc acggcctgtc gtgagcctgt
      421 atccgctcag catacacatc aggccatatt ccagatatct gtcacgggtc ataataataa
      481 tctgcatact ctcagaccct tatctcccgg ataccggctc cgtttaccat taccggtata
      541 actgactcat cattcataac ggaaactgtc tgccgcatac atcagtgccc gctcttccag
      601 ctctgacaga ctcagctctt tcagttcccc ttctccttcc gcaagaagac aacattcaaa
      661 aacagctgca tcgtcgcatt ccagacgaac ctgcaccctg tcagcccgac cgtttacagt
      721 aaatacctgc actgaactta tgcgatagtt cagcatatac actctccctc tcagctcctc
      781 aggaataccc cggaaccgtc agtcatgcca ccggggagaa aagcaggcag cgaatcatga
      841 ccgcatacag aacgctgccc gccccggtga ctcagacccg ggttcagaac tcccagttga
      901 cacccacatt ccaggaaaca tcaccgtcac tgaacgcagt acctgccttt gccgcagcct
      961 gctcgttaaa gcgatatccc atgcccaccg caacagcctg cctgtcactg tacccgccca
     1021 cagccgctgt ggcgttaaat ttacccacac tgtatggctg gaaaagaccg gccagcgcag
     1081 ctgactgggc tgctgcccgc ttcatttcct catgattctc attaatctga cgctgctgac
     1141 tgttcatacg acgctcatgc gcctgcaggg tacgtgcctg agaagcaatg gcgcgactgt
     1201 tgcgttcacc cacctgacgg ttacggctga ccgcagcacg gttttctgtg gccatttcct
     1261 gtgtacgtac cagcccttcc tgtacagtaa ttcgttcacc ggttccctca ccgcgcgtca
     1321 cataaccatg actgtccaca gcaacggtac ttccggtatt tatctgctgt tgctggctgt
     1381 tcaggcgctc ttcatgcgtc tccagcaggt ctctctggga aagcagtccc cgggtgttac
     1441 ggtttatggc caccgtgttc cggataatat tctccctgtt ctctgcagca accgtatcgg
     1501 tatggtcacg cgccttaagt gcttccgctg caatccgtcc actgacgtca ctgtacacct
     1561 catccgcctt cctgttcagt tgcccgacag tggctgcatc cgtatcccgt gtaccatcac
     1621 tgacaccacc gagaacccgg gttttatccg gtacacgcgg actggccggg tttgtgacac
     1681 ctgagtcata tccgatgtag acttcatttt cacgggttgt accggactgc tgaccaagag
     1741 ccactgaata cctgtgcatt gcctgtgcac ccgtacccag tgccaccgaa tttaaagcat
     1801 cagcctttgc ccagctacct actgccacac cattagacgc cttcggattt acgtaagctg
     1861 acatccccac ggatgtggaa aacggcgcat gcgcatacga tgcagagcca agagctgtcc
     1921 ctgcctttcc agccctcgcc catgcaccta cagccgtgga gctggtctct gatgcagagg
     1981 ctgtgcccgg agacgcccag gaactgtcat cctgcgcaaa cgccggagca ccggcaatca
     2041 gaaaggaaat aagcgataaa gtaataccat gtcgatttat tttcataaac aacctcattg
     2101 atatacacaa tatactggca cagagttaaa ttaatatata atcaggagag aagtgaaaga
     2161 gtacccggtg cgaaaatgaa ttaatctgag cagatttaac ggtaaacgaa gcgcaataaa
     2221 ttaaaattaa aaaaacaaaa tcacttgcag tctattatct ttacgttcct caccgcaacc
     2281 gcacacaaaa acaaaacaca caatgcattg attttaaaca aaaaaaacaa aacagaaact
     2341 caacacataa aaatcagaca atataaacag aattgtgatt tatatcctca agcgctgtat
     2401 taacgaaata tatccgtcct cctgcaatac ctgcctcaac atcatttccg gtttgtttca
     2461 gacatgaaat gaactcatct gtcgatgtaa caatgtcctt tgataaaaac agaaaatctt
     2521 tttcaccctt atgtttatta aaatccatga taaatgaatt cccttcacaa gcagcatcaa
     2581 agacgaagac atcatgacca gtaccaccat acagataatc attacctccc ccaccatata
     2641 accagtcgtt accttctgct ccgtacaact catcattacc agaagcccca tgaagataat
     2701 cattcccgcg acctccgtgt aaaagactta ctccttctcc cccggcacta tgtaatatat
     2761 catttccgtc aagaccaaaa ataatatttc cgaacagact atcatctccg tttgtacctt
     2821 ctgtatgttt tccgtaatca acatttttat tacgccactt cagaagatac ctgtggtctg
     2881 taatctgctc ttcttctatc tgataagggc ttagtttact cctccagtca ccagaaaaag
     2941 agataccttc aatacctgtt gcctcagtca gaccggctcc ccgttctgaa tggaaaaaca
     3001 gcgtcccatc actgaccctg tatatattcc agtcatccat tacatcgcta agaagcagcg
     3061 tgtcataccc actgccacca tcaaccctgt ttgctccctt acctggttc
//
LOCUS       DAZGAN020000246         2995 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGAN020000246 DAZGAN020000000
VERSION     DAZGAN020000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.248"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..848
                     /locus_tag="ACHKYO_RS25360"
                     /old_locus_tag="pgaptmp_005072"
     CDS             <1..848
                     /locus_tag="ACHKYO_RS25360"
                     /old_locus_tag="pgaptmp_005072"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGU4431727.1"
                     /translation="NNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLIDTAAVYGN
                     EDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSGLEYFDLY
                     LLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMVNQVELHP
                     YFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQVILRWNIQ
                     RGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNPEFVRGCL
                     AVKNS"
     gene            complement(1012..1605)
                     /gene="rclC"
                     /locus_tag="ACHKYO_RS25365"
                     /old_locus_tag="pgaptmp_005073"
     CDS             complement(1012..1605)
                     /gene="rclC"
                     /locus_tag="ACHKYO_RS25365"
                     /old_locus_tag="pgaptmp_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HGU4431728.1"
                     /translation="MEKYLHMLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(1617..1853)
                     /gene="rclB"
                     /locus_tag="ACHKYO_RS25370"
                     /old_locus_tag="pgaptmp_005074"
     CDS             complement(1617..1853)
                     /gene="rclB"
                     /locus_tag="ACHKYO_RS25370"
                     /old_locus_tag="pgaptmp_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HGU4431729.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(1962..>2995)
                     /locus_tag="ACHKYO_RS25375"
                     /old_locus_tag="pgaptmp_005075"
     CDS             complement(1962..>2995)
                     /locus_tag="ACHKYO_RS25375"
                     /old_locus_tag="pgaptmp_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4431730.1"
                     /translation="AEFINNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTT
                     PGVYDSTGLLNLKELPGHLGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRD
                     IADNIATILRDQGVDIILNAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATAS
                     LHPENAGIAVNERGAIVVDKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLG
                     EGRRSTDDRKNVPYSVFMTPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDT
                     RGVLKAIVDNKTQRILGASLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSES
                     LNDLFSLVK"
BASE COUNT          856 a          654 c          648 g          837 t
ORIGIN      
        1 gtaacaatct gaaaatgccg atgatgggat ttggtgtttt tcaggttacc gataaaaacg
       61 tgtgcaaaca gtcagtgttg aacgctatcc gcacgggtta tcgactcatc gacactgcag
      121 cggtatacgg taatgaagat gccgtcggtg aagccgttcg tgaagctata tctgaaggtt
      181 tatgtacccg agatgagtta tttataacgt ctaaattgtg ggggcaggat atgttgaatc
      241 aggatacggc agcagcaggt attgaagcat cattaaaaaa atcgggacta gaatacttcg
      301 acctctattt attgcaccag gctatgcgcg attatttcag tgcgtggcgt gcactcgaag
      361 atgcctatga agagggcaaa ttaaaagcaa ttggggtttc caatttctat cctcatgttc
      421 tggcgaactt ttgtgaaacg gtaagagtta aaccgatggt caaccaggtc gagttgcatc
      481 cctattttgc ccaaccagag gcgctggcaa ccatgaagta ttataacgtg cagcctgaag
      541 catgggctcc gttaggtggt ggacgacata aaccctttga aaataatctg cttcagagta
      601 ttgcagatgc ccatcaaaaa tcgatttctc aagtcattct gcgttggaat attcaacggg
      661 gagtggtcgt tattccgaaa tcgacacatc aacagcgtat cgaagaaaat tttgctatct
      721 gggatttctc actgacagag aaagaaatgg cacaaattag ttcgcttgat ttgggttatg
      781 ttggggagtc ggtaaaacat tttaatcctg aatttgttcg tggttgtctt gctgtaaaaa
      841 attcatgatt gatattaatc atatatttta cctgagacga caagaatctt ttaacagggg
      901 agtgatattg atcttcactc tgtcatatct ccggtaatat ggcgtcaggc ttcatcatca
      961 gaaagggggc ctgacgcctg aaaaagtgaa caacagacag tgttcggatt atcaatattc
     1021 agtttttaac gttgaactgg attcattact gcgttgttta agaatttccc gcgccgaatc
     1081 tgccattatc atgacagcac ctgccagcat cagagtatct ttcaatacca ggcgaccagc
     1141 accggataaa taagggaaac catgatgagc gtcacccaat gcgggtaccc atgcctccgg
     1201 ggtggtgatt aaaaatgaga gtgttaccaa cggtgtggta aatgccatca gccctcccaa
     1261 taaacctaac cagcgattga caggattagc caaaaccagc agagcaataa tcacctccac
     1321 gacgccaaga ccgttggaaa aaccataggt attattggcc gattgccatg cccttgcttc
     1381 tggtttgtat tcgccttcgt gagtcagata ctgtttatag tcttccgggt gttcataaaa
     1441 gaacgacatt agtggactgt ttgcgacgaa tggtgtaatg ctgtctgcct cgtaagggac
     1501 aaactttaat aacccaatcc acataaaaac aatcgcaata ctcagacgaa tcaatgtcag
     1561 gccaatttta tctccccgac ttaacatgtg caggtatttc tccattgtaa acatccttat
     1621 ttatacaata ctgctgttgc ggttgaatta ttatcaattc gcatcgatgt gactttccag
     1681 gatgaagcat tatattttct ggctatatca gcaatggcat cctccagaac tgtcggtgat
     1741 gctgatactg acgagacgct gatatgtttc agaattattt tatcatctgc attggtggaa
     1801 aatgccataa cgccagataa tagtgttgca aataaaacag attttttgaa catataaaat
     1861 accatcaatg aagtgattaa gaagttatag agtaacaggg aattaataag attcttttct
     1921 ctgagacgcc agaatatttg ttctggcgtc tgattttgag tttatttgac taatgaaaat
     1981 agatcattga gtgattcgct catcgacgga tgagtaaata tctgatcgcg taatatgcta
     2041 taaggcagcc cggcatccat caccattttc actatattga tcatctcgtg ggagtcaaca
     2101 cacagcagtg atgcccctaa tatacgttgg gttttattat caacaatcgc ttttaatacc
     2161 ccacgagtat cattcatcac tctggcacgc ggaattgcag ctacaggcaa tgtcaccacc
     2221 tgaatatcag caccactctc tctggcctgt tcttctgtca taccaaccct ggacagtggc
     2281 ggtgtcatga atacggaata aggcacattt ttccgatcat cagtactacg tctgccttct
     2341 cccagcaact catcacgcac aatgcggtaa tcatccagtg aaatataagt aaattgcagc
     2401 ccgccggtaa catctcccat cgcccaaata ttgtctgcgg tggtatgtaa ttgcttgtca
     2461 acgacaattg ccccgcgctc gtttactgcg ataccagcat tttctggatg taacgaagcg
     2521 gtagccggtt gacgaccgga agctattaac agtgcatcca ccgccagttg ggcgtgctcg
     2581 ctatgcactt gcacttgatt ttcatggtga ctgattcgct ccacatgggc attgaggata
     2641 atatcgacgc cctgatcgcg taaaatcgtc gcgatattat cagcaatatc ccgatcttcc
     2701 cgaggcaaaa acagcgaagc tgcttctaaa atggttacct tgctgccaaa attagcgaac
     2761 atagaggcga actcaacgcc aatatatccg ccgcccaaaa tacctaaatg cccaggcaat
     2821 tcttttagat taagtaatcc ggtgctgtca tatacaccag gcgtggtggt aattccagga
     2881 attggcggaa ccacggtttg tgcaccggta ttaataaaaa ttttctcgcc atgaatttcc
     2941 agatttccct caggccgatg aacacgcagg ctatgattat tgataaactc cgcct
//
LOCUS       DAZGAN020000247         2898 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGAN020000247 DAZGAN020000000
VERSION     DAZGAN020000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.249"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(243..1691)
                     /gene="cusS"
                     /locus_tag="ACHKYO_RS25380"
                     /old_locus_tag="pgaptmp_005076"
     CDS             complement(243..1691)
                     /gene="cusS"
                     /locus_tag="ACHKYO_RS25380"
                     /old_locus_tag="pgaptmp_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HGU4431731.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            complement(1681..2364)
                     /gene="cusR"
                     /locus_tag="ACHKYO_RS25385"
                     /old_locus_tag="pgaptmp_005077"
     CDS             complement(1681..2364)
                     /gene="cusR"
                     /locus_tag="ACHKYO_RS25385"
                     /old_locus_tag="pgaptmp_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HGU4431732.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLMKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLQE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2521..>2898
                     /gene="cusC"
                     /locus_tag="ACHKYO_RS25390"
                     /old_locus_tag="pgaptmp_005078"
     CDS             2521..>2898
                     /gene="cusC"
                     /locus_tag="ACHKYO_RS25390"
                     /old_locus_tag="pgaptmp_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HGU4431733.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFST"
BASE COUNT          725 a          749 c          728 g          696 t
ORIGIN      
        1 attaaattat tcattgcttt atatgataga tggcttgttt ataacgatga aaatttagaa
       61 agggcattgt gatctacaat tggaagaaac acagatttaa ctaagtttca tatacaaaca
      121 tctcgtgaaa ctttgaaaaa taaaagcctt cgatatttca tatgtttaat aagtttttct
      181 ctaatttgtt gatcaacaat accaggaggt gatgcaccac gccctgaatt tgaacttaat
      241 aatcactctc tctccggcag cataatcaca aaccttgtcc cccgcgcatc tgacgttacc
      301 gcaaccgtgc ctttatgcgc gacaacaatc gatttcacta tcgccagccc aataccgcta
      361 ccttcacctt ttcgctggcg ggaagggtca acgcgataga aacggtcaaa caatcgcggt
      421 aagtgctcgg gcgcaatggg cgtaccgggg ttttcgacgg aaacttgcac ctgatgattg
      481 accgtctggc agcgcactac aatagcctca ctgggtggcg tataacgcag ggcattagaa
      541 agcaggttgc ttaacgcccg acgcagcatc agcggatcgc ccgcaacctg acattcgtcg
      601 ccaacaaatc gcaactccac gccgcgatct tccgctaacg cctcgaaaaa atcgaacact
      661 ttgccgactt catccgccag gttgagcatt ttcttttcgg ggattagctg gttgttatcg
      721 gcctgagcga gaaacagcat atcgctgacc attttcgcca ttcgcgtcag ctcttcgaga
      781 ttagagtaga gcacatcttc cagctccttc tggctgcgag actggctgag ggcgatttcc
      841 gtttgcgtga tgagattggt aatcggcgtg cgaatttcgt gagcgatatc cgctgagaaa
      901 ttggactggc gggtaaagac atcctcaata cgctcgatca tatggttgaa cgacagtacc
      961 agctgttcca gctcaatggg cacggtctgc gggtcaaggc gaacgtcgag atctttcgag
     1021 gtgatattct ggatttgacg gctgacgctg cggatcggcg cgtgaccttt atgtaccgcc
     1081 aacagcacga taaagacgat caggatgctg attaccgatg cggtcataat aagtttattc
     1141 atcaaatcat ttatgtaatg aagatgaaaa tcgatcgaaa gcgcgatgta gagcgtataa
     1201 atcggtttgc cgtcaaccag cgggccaacc ggtaagttaa tcatccgcca gttgctgtgt
     1261 tccatatgcc cgtgaccgtg gcctggcatc ataatcgtcg ggccggaaag gagatacacc
     1321 tcgccaccct gagcgtcttt atcgggtatg gcgtcacgcg taaactcgcg gatatccggc
     1381 gcaccggggg agtgatacac cgttttgccg tggctatctg ccagggaaat caacacgttg
     1441 gaataaccac tgacgatatc ttccagcgtc attaagcgtc gggcttgcgt ctcgtcagga
     1501 tgattaagta cccgttcaag ggtggcgcta atttctttta aatcattaat atcctgctcg
     1561 gcaaaatgca ctttcactga gtggatcatg atccatgcaa aggcgaaaaa cgccgcgatg
     1621 gtggccaggc tgataaaaaa ggtcagacgg gttgccagcg aaaacgggcg ctgaaatggc
     1681 ttactgacca tccggcacct caagcatata gcccacgccg cgcacggtct gaatcagctt
     1741 cggctcaaag tcgttgtcga ttttgccgcg tagccgcttc accgccacat caatggcgtt
     1801 agtgtcgctg tcgaaattca tgtcccacac ctgcgaggcg ataagcgagc ggggcagcac
     1861 ttcgccctga tggcggagaa aaaactcctg cagagtaaac tctttactgg tcagagtgat
     1921 gcgcgtgccg ctgcgggtga ctttgcggct gacgagatcg accatcaggt cggcaacctg
     1981 aaactgactt tcgataatta ccgccgcccc gcgccgcagg agagtgcgca cccgcgccag
     2041 cagttcagca aaagcgaatg gtttcatcag gtagtcatct gcccccaact ccagcccctt
     2101 gacgcgatgt tcaatggtgc caagcgcggt aagcaacaga atcggcatcc ctttattggc
     2161 ggagcgtaac atgcgcacga tatcccagcc gttcacgtcc ggcagcataa tatcgaggat
     2221 tatcagatca taatcaccgg tcatcgccag atggtagcca ttcagcccgt tgtcggccaa
     2281 atcgaccaca aaaccggctt cggttaaccc tttggtcaag tattctccgg ttttcttttc
     2341 atcttcgaca atcaacagtt tcatttttcc tccgcatgtt gcccgggcaa ttctagagta
     2401 gcgggatcag atggcaatcg cttattggca aaatgacaat tttgtcattt tcctgtcacc
     2461 ggaaaatcag agcctggcga gtaaagttgg cggtataaaa tcaccagaaa ttatgagcct
     2521 atgtctcctt gtaaacttct gccattttgt gtggcccttg cgctaaccgg ttgttcactg
     2581 gcaccggatt atcagcgtcc ggcaatgccc gtgccgcagc agttctcact cagccagaac
     2641 ggcttggtta acgcagcaga taactaccag aacgcgggct ggcgcacctt ttttgttgat
     2701 aatcaggtaa agacgctgat tagcgaggcg ctggtgaata accgggattt gcgcatggcg
     2761 acgctgaaag tgcaggaagc gcgggcgcaa tatcgtctga ccgatgccga ccgctaccca
     2821 cagctcaatg gcgagggcag cggcagctgg agcggtaatc ttaaagggaa taccgccacg
     2881 acacgggagt tttcgacc
//
LOCUS       DAZGAN020000248         2867 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGAN020000248 DAZGAN020000000
VERSION     DAZGAN020000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.250"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..195)
                     /locus_tag="ACHKYO_RS25395"
                     /old_locus_tag="pgaptmp_005079"
     CDS             complement(<1..195)
                     /locus_tag="ACHKYO_RS25395"
                     /old_locus_tag="pgaptmp_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431734.1"
                     /translation="MKKLLVTVKSFQGTIPFRILQCGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRM"
     gene            complement(281..466)
                     /locus_tag="ACHKYO_RS25400"
                     /old_locus_tag="pgaptmp_005080"
     CDS             complement(281..466)
                     /locus_tag="ACHKYO_RS25400"
                     /old_locus_tag="pgaptmp_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4431735.1"
                     /translation="MRELKMKLCVLMLPLVVSACGLKPPVQAPCVKPPPPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            complement(683..1618)
                     /locus_tag="ACHKYO_RS25405"
                     /old_locus_tag="pgaptmp_005081"
     CDS             complement(683..1618)
                     /locus_tag="ACHKYO_RS25405"
                     /old_locus_tag="pgaptmp_005081"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGU4431736.1"
                     /translation="MKCVLYSGDCLDVMPSLLPGSVDLVLCDPPYGTIKGLTLRTWDK
                     STTAWDDALNPEDIFTQCERLLRVGGVLVMFSQEPHTSTMITLADKNLPFLYRMVWKK
                     EHFPNPLSVKKAPVSLFEDIVVFRKKYDSEKNHPLREYARNILSFIGHRTDKEVNKTL
                     GHRRAEHFFCVNTLQFSLCSQDTYNELIKRFGIDRMPGFMTYEEMRKVNSGYGTVVFN
                     LPQGKKYRANILEYARERERWHPTQKPVALLEELIQTYSNPGDRVLDFTMGSGSTGVA
                     CIRTGRRFTGIEKDDRYFAVAKKRLEKENSEMVVI"
     gene            complement(1626..1757)
                     /locus_tag="ACHKYO_RS25410"
                     /old_locus_tag="pgaptmp_005082"
     CDS             complement(1626..1757)
                     /locus_tag="ACHKYO_RS25410"
                     /old_locus_tag="pgaptmp_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431737.1"
                     /translation="MSDKLIMLAKILCVIVGISFSLMLVAICFSTAWRVLTLSGLVG"
     gene            complement(1772..1956)
                     /locus_tag="ACHKYO_RS25415"
                     /old_locus_tag="pgaptmp_005083"
                     /pseudo
     CDS             complement(1772..1956)
                     /locus_tag="ACHKYO_RS25415"
                     /old_locus_tag="pgaptmp_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2113..2646)
                     /locus_tag="ACHKYO_RS25420"
                     /old_locus_tag="pgaptmp_005084"
     CDS             complement(2113..2646)
                     /locus_tag="ACHKYO_RS25420"
                     /old_locus_tag="pgaptmp_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4431738.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            complement(2683..>2867)
                     /locus_tag="ACHKYO_RS25425"
                     /old_locus_tag="pgaptmp_005085"
     CDS             complement(2683..>2867)
                     /locus_tag="ACHKYO_RS25425"
                     /old_locus_tag="pgaptmp_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431739.1"
                     /translation="RIGRCMGYLQVAAEIEALASGADKDSVVREALLRDFDNPPFKKV
                     PAYWLHPGLTYLKVRI"
BASE COUNT          705 a          802 c          618 g          742 t
ORIGIN      
        1 catgcggcat ttagcggcat tcatcgtaca ctcaacggtt agctcttcat tcgtggcatt
       61 cacctgaaag gtccgggagt gtaattgcgt acatttacca ctgaacgaac cttcaacaag
      121 aacacgacca cactgcaaaa tacggaacgg aattgttccc tgaaaagact ttacggttac
      181 cagtaatttc ttcatgcatt ctccggataa caaaaatact agctaataca ctgagtgcgg
      241 atatattcct gcgccccttc cagctgcttc tgcattgtca tcaaccgttc tctgaggatg
      301 aaataatccc gttcagcggt gtctgccagt cgggggccgg ttgcattatc cacgccggag
      361 gtggtggggg cttcacgcac ggagcctgga caggtggctt taacccgcag gcgcttacga
      421 ccagcggcaa catcagcacg cagagtttca ttttcagctc tcgcatcggc taattccctc
      481 gagtattttg catcgagcgc agcaacatcg cgctggcgca cctgcatatc agtaatggtt
      541 gcgtttgcca gctccagctc tctggctttt ttatcgcgct gcgctttgta ggtgatggcg
      601 ttatcgcggt aatgattcag ccccagacta agcgcaccac aggccaccag caggacaata
      661 atcaccacac acagaacacg gttcatatca ccaccatttc actgttttct ttttccagac
      721 gtttttttgc aacagcgaaa taccggtcat ccttttctat tccggtaaac cgtctccccg
      781 tccggataca tgcaacacct gttgaaccac tccccatagt aaaatccagt accctgtcgc
      841 cagggttgct gtacgtctga atcagctctt ccagtaatgc aactggcttc tgggtcggat
      901 gccatcgctc ccgctcccgc gcgtactcca gaatatttgc gcggtatttt ttcccctgag
      961 ggagattaaa aaccacagtg ccgtaaccgg aattcacttt gcgcatttct tcatatgtca
     1021 tgaaacccgg catacggtcg ataccaaagc gtttaattaa ctcgttatac gtatcctgtg
     1081 agcagaggga aaactggagt gtgttcacgc aaaaaaaatg ctctgcccga cggtgcccca
     1141 gtgttttgtt aacttccttg tctgtcctgt ggccaatgaa agaaaggata tttcgggcgt
     1201 actccctcag cgggtggttt ttctcactgt catatttttt ccggaaaaca acgatatcct
     1261 cgaataaaga tacaggtgct ttttttactg aaagcgggtt ggggaaatgc tccttcttcc
     1321 acaccatacg atataaaaac ggcaggtttt tatccgccag agtaatcatc gtgcttgtat
     1381 gcggctcctg gctgaacatg accagcacac cgcccaccct cagcagtcgt tcgcactgtg
     1441 tgaaaatatc ttcaggattt aaggcatcgt cccatgctgt cgtactctta tcccatgtac
     1501 ggagtgtaag ccctttaatg gttccatacg gcgggtcaca taaaaccagg tcaacacttc
     1561 ccggtagtaa tgaaggcata acatcaagac agtcaccgga atacagtaca catttcatgt
     1621 ttctgtcacc ccaccagccc tgacagagtt aagacgcgcc aggcagtgga aaagcaaata
     1681 gcaaccagca ttagtgaaaa tgaaatgccg acgattacac agaggatctt cgccagcatt
     1741 atgagtttgt ctgacatgct taatcctccc ttcacgattt caacgcaatg accagttttg
     1801 ccagcccata cagtatcgga gacacagcga taccaaccgc cacccactta atagcaaaag
     1861 ccagcgctct gctgatgtca tcagttactc tggcgctttc agttcaaggc cgtttttcat
     1921 agtcaacctc aacagaattc gtttatactt ttccatgttc tcccttgcct tatccaaggt
     1981 cagaaacaca aaaccccgct tgctgccaac aaacggggtt tttactttta ttcacttagg
     2041 ttttgccagt tcgcaggatt tcgtgttatc cgtccgcgtt ggtcaacgtc atttttcagc
     2101 aaaatattct gcttatctgt cgatacccca gcacgccagc gcgctctcct ggtcacgacg
     2161 ggatacctga ccataacagt tgtttgagcg gatacggcag tctctgccac cgtccttaat
     2221 ccaccagcga atcgcttcac acgctcccct gcgatcgcct gcattaattc gtttataaaa
     2281 cgtcgacggg aaacacttac ccgggccaat gttgtaagga cagaatgacg caatacccgc
     2341 tttctggggt tcagtcagtg gcactctgat gtttttctcc acccatgcca gcgccttatc
     2401 acgctcaatg gcgttaacct ggtcgcattt ccccttcgac agcttcatgc caggaataac
     2461 aggcttacca tccacccggg tggctccacg gcagatggtc cagatacccg cgccatcacg
     2521 gtatgccgtg gtgtggttac cttctttttc atccagaaac tggtcgagga tttcaggcgc
     2581 agaagcccct gcggcaatca acgccagaac ggcagccgac aggccgtatt tgatttttgt
     2641 gttcatggat atattaaata ttcagccgct gtcccaggcc cactaaatac gcactttcag
     2701 ataagtcagt ccgggatgaa gccagtaagc cggcactttt ttaaagggcg gattatcaaa
     2761 atcacgaaga agtgcctccc gcacaactga atccttgtcc gcaccactgg ccagcgcttc
     2821 aatctcagcg gctacctgca gataccccat gcaacgacca atgcgct
//
LOCUS       DAZGAN020000249         2854 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGAN020000249 DAZGAN020000000
VERSION     DAZGAN020000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.251"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(407..1366)
                     /locus_tag="ACHKYO_RS25430"
                     /old_locus_tag="pgaptmp_005086"
     CDS             complement(407..1366)
                     /locus_tag="ACHKYO_RS25430"
                     /old_locus_tag="pgaptmp_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGU4431740.1"
                     /translation="MHSEELKNTLGLAIQIGKLASDQSVLKFFELIDFEKVRDLRDLT
                     QVVNQYGLLKGNISETDFLIASAELESHYSQGVEPVPYGSEYYPLSLVFTPNPPAILY
                     VKGGKHILSELPGVAIVGSRDTSPAGEEITRRITNQIVAAGFIIVSGLAIGTDANAHK
                     AALQAKGKTIAVLAHGLEKAKPKQNSRLAQEILEKGGAWVSEYPIGRPAQKQSFVQRN
                     RIQVGLSVGSILIEAALNSGTMTQAEFANKANRPVFAVIPHLPDNPLNLNCEGTLDLV
                     RNNMARPLKTKRDYENVISIMNESRKKLLGLKYPQKQNTLDLI"
BASE COUNT          886 a          647 c          471 g          850 t
ORIGIN      
        1 atcagccata taataaaata atatttggtt gccgtttaca aatgagtaaa aagatgtttc
       61 cataatgatt cgcattaatc cacaaatagt tattactggt tggatcactt tgtaaaagac
      121 tctaattagt attatgcttg caagataaat agtaaggata gaagacattt tcttgtttta
      181 acctttaacc ttcaatcatc aaccttaaat atttagcata aacatactgg catctaatat
      241 ttatctcaat gaaaaactac tgtagcaaat cggtttagca gctattatca aaaggactta
      301 cgactaaaat actcagacaa agaataaaag acttgaatac gtgacttaac catgaactaa
      361 taaattttga agatcattgc atctaaaaaa gcctcttatg aggcctctaa attaaatcta
      421 aagtattttg tttttgcggg tattttagcc ctaacaattt cttacgagac tcattcatta
      481 tagaaatcac gttctcataa tctcgcttag tttttaacgg acgagccatg ttatttctta
      541 ctaagtctaa tgttccctcg caatttaaat tcaaaggatt atcaggtaaa tgtggtataa
      601 cagcaaaaac agggcgattt gctttatttg caaattctgc ctgagtcata gtgccgctat
      661 tcaatgcagc ttcaatcaga attgatccca ctgataatcc tacctgtatt ctattccttt
      721 gaacaaaaga ctgcttttga gccgggcgtc caataggata ttcagaaacc catgctcccc
      781 ctttctccag tatttcttga gcaaggcggc tattttgctt tggctttgct ttttcaagcc
      841 catgagccag aacagcgata gttttccctt ttgcctgtaa tgccgcctta tgagcattcg
      901 catcagtacc tatagctaat ccactcacaa taataaatcc agcagccaca atttggttag
      961 ttattcgcct tgttatttct tcaccggcag gtgatgtatc acgagagccg acaattgcta
     1021 caccgggcaa ctcagataat atatgcttcc cccccttcac atacaaaatt gcaggaggat
     1081 ttggtgtaaa gactaaactt aagggataat attctgaacc atatggaact ggttccaccc
     1141 cttgagaata atgactctca agctctgcgc tagcaatcaa aaaatcagtt tcagatatat
     1201 ttcctttcaa caatccatac tgattaacca cttgtgttaa atctcgcaaa tcacgaactt
     1261 tttcaaaatc aataagctca aagaatttca atacactttg atcagatgct aacttaccaa
     1321 tctgaatagc taaaccaagt gtatttttca attcttcaga gtgcacagca ttactcctca
     1381 tagtttgccg tttttaataa cgacagtgga attataactc ctgcacccgc agattccagt
     1441 agatagaagc aagcagacag gcttcctcct gtcgtcttaa catcatccag taataacaca
     1501 tttcgcccat gcaggttacc acctatgact ctaatagtag acatatgtcc agtaattgag
     1561 cgatctccac cttctcgatg ggcactcggg acactgcgaa accgttccag acaacaattc
     1621 acatctccac atcgataaac tcgtactata cgctcagcca cctcaatcaa tgctggtgac
     1681 actcgcccag cagtatggga aggtacaaca gcaatagaaa agggatgctc gacaaaatac
     1741 tcgtgtggtc gtcctggaat tctcaatcct ctaactgttt cgatagctaa ttgagaaaaa
     1801 cgattaatag ctcttcgata attacggtgt ccttcattct taaaatccat catgagccta
     1861 gagatgtcgt cacaacgagg atttcgttcc cctaagtact tataaggatg atatacaccg
     1921 catgaagtca cgcttacggg tagaagcatt ctttaatctc attttgcctt gttattcgtt
     1981 atgttaagcg aaacgacatt tcaaaaacaa actgaaaatc aagaataatc ttacaagtca
     2041 catttttaac atagcactgc cagcgcgcct cttatccctg acacgcctgc gcgctttatg
     2101 tagtggtttt catgcgactg catatcataa gcaaaagcct gccattcctg atgggcctca
     2161 gctaaaacgc tcctcaaacg atcatgtgga ttcatgcggt atgacatgta caatcgccaa
     2221 agagggcatc gagacttttt ggaaacatac gtaaccacag ttaatatgtg catccagtct
     2281 cgaattgcat cacatgtctc atcaaaaagc ttggtgatgg ttgtacggtt caactccgcc
     2341 tctttccaat aattttagct gtcaaattct ggatcgccat gctgaaacga ttttgactct
     2401 gaaaggtatt ccttccggaa acggtccaag gttatataaa cctctggtga atgaacgttt
     2461 tgttcgactc accacttgta acaaaagtat taaatgcacc tgaaaaaact ttcgccttca
     2521 aatggttcgt tgaggtcaaa atgatccagc gcagtcctca gcttaagcac gctttctctg
     2581 gtttcaaaca tcgaattcca aatgagacta tatgctgata actccttatc aaaatgcgct
     2641 ttcgtgacat aaacagaatt gtctaactta gcctttactc tttcattagt tgcactcact
     2701 tgtgagtgaa attcagcaat atttttgttt agccgcgact gatatctctc taaataaact
     2761 ttcccgaccc aaataactat gccagatgca ataaaagacc gccccccaac gaagccagaa
     2821 ctttaaagac caaatccatt gtgcccacct gcaa
//
LOCUS       DAZGAN020000250         2823 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGAN020000250 DAZGAN020000000
VERSION     DAZGAN020000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.252"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(112..303)
                     /locus_tag="ACHKYO_RS25435"
                     /old_locus_tag="pgaptmp_005087"
     CDS             complement(112..303)
                     /locus_tag="ACHKYO_RS25435"
                     /old_locus_tag="pgaptmp_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HGU4431741.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            371..694
                     /locus_tag="ACHKYO_RS25440"
                     /old_locus_tag="pgaptmp_005088"
     CDS             371..694
                     /locus_tag="ACHKYO_RS25440"
                     /old_locus_tag="pgaptmp_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HGU4431742.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            903..1337
                     /locus_tag="ACHKYO_RS25445"
                     /old_locus_tag="pgaptmp_005089"
                     /pseudo
     CDS             903..1337
                     /locus_tag="ACHKYO_RS25445"
                     /old_locus_tag="pgaptmp_005089"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1372..1932
                     /locus_tag="ACHKYO_RS25450"
                     /old_locus_tag="pgaptmp_005090"
     CDS             1372..1932
                     /locus_tag="ACHKYO_RS25450"
                     /old_locus_tag="pgaptmp_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HGU4431743.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            1939..2172
                     /locus_tag="ACHKYO_RS25455"
                     /old_locus_tag="pgaptmp_005091"
     CDS             1939..2172
                     /locus_tag="ACHKYO_RS25455"
                     /old_locus_tag="pgaptmp_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HGU4431744.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
     gene            2324..2725
                     /locus_tag="ACHKYO_RS25460"
                     /old_locus_tag="pgaptmp_005092"
     CDS             2324..2725
                     /locus_tag="ACHKYO_RS25460"
                     /old_locus_tag="pgaptmp_005092"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HGU4431745.1"
                     /translation="MSEQEEREQKRREYRALLEGFWPDAFNFSRPRPLKVGILAELIA
                     DAEQRGLPFDSELLTSAIKFYTRRYVYQRALTTMKERTGLDGQIAGEVTPKQREYSLK
                     LIRRFAAKDKAEKRARQEEKAAKQSGINPGA"
BASE COUNT          695 a          638 c          925 g          565 t
ORIGIN      
        1 catggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg cttgtctgct
       61 cccggcatcc gctcacaggc aagctgtgac cgtctccggg agctgtatgt gtcagaggtt
      121 ttcaccgtca tccccgaagc gtgcgaggca gctgcggtaa agctcgtcgg cgtggtcgtg
      181 aagggcttcg cagatgtccg cctggtcgtc ggcgtccagt tcattcagtt tttccagcag
      241 tgtcagcgtc tggcttctga taaagcgggc catgttaagg gctgtttttt cctgtttagt
      301 cacagttctg tttctccatg tgtggggagt ctgtcggagg tttttaatga taccggtgaa
      361 acgcgaaagg atgctcacta tacgggtcac ggatgaggaa catgcgcggc tgctggcacg
      421 ttgtgaggga aaacaacttg cgtcatggat gcgtaaggtc tgcctgggtg caccgccatc
      481 gaaaacgtca ggtcttccga cactggcacc gccgctgttg cgccagttcg cttcggtcgg
      541 caataacctg aatcagatag cccggaagat aaacagcgga cagtggtcgg ggcatgaccg
      601 ggttcatgtg gtggctgcac tgatggccat cggacgtgaa ctgagtgagc tgagggatga
      661 ggtccgtaaa cagggagaac gggatgatag ttaagtttca tgccagggga aaaggcggtg
      721 gcagtggtcc ggttgattac ctgctgggcc gggagcgtaa ccgggaaggg gcgagagttc
      781 tgcggggtgc tcccgaagag gtgcgggaac tcatcgatgc gacgccgttt gcgaaaaaat
      841 acacctcggg ggttctgtct tttgcagagc agaccctgcc accgggagag cgtgaaaggg
      901 tgatggagag ctttgagcgg gtactgatgc ccggtcttga aaaaaatcag tacagcatcc
      961 tgtgggtgga gcaccaggac aaggggcgtc ttgagctgaa ttttgtcatc ccgaatatgg
     1021 agctggcgag cggaaaacgg ctccagccct attacgaccg ggcggaccgt ccgcgaatca
     1081 atgcctggca gacactggtg aatcaccatt acgggctgca cgacccgaac gcaccggaga
     1141 acaggcggac acttgtcacc ccgaataatc tgccgaaagc gaaacaggag gccgcagaag
     1201 cgattacgag gggactggag gctctttacc atgccggagc gctaaaaacg cgtcaggatg
     1261 tcactgaggc gcttacagcg gcaggttttg aggttgtgag gacaaccagg agcagtatca
     1321 gtatcgccga cccggagggt gggcgtaatc tcaggctgaa aggggcgctg tatgagcaat
     1381 cttttacaaa tggggacaga cttcgagaag aaactgaaag agcgtgccgt ctctacagag
     1441 aacatgctga acagcgagtt caggaagctc gaagcgtctg tagacaggga actgaactta
     1501 aacgggcaga aaatcagaga cgctatcagc gcacacacag cagcggtgaa agaacaactg
     1561 gagacgctga acagtgccgt gaacgcgcag ttcagcgcca cagagaacga actggcccgg
     1621 cggcaggaag agctgctctg gaagctggtc aaagggcgga tactgtaccc gtcgctgacg
     1681 gcgctttgcg tcacgggcgg gatttttctg gcgtcatggg ggctgattca gtggcaggaa
     1741 agccggattg cggcgaacat cctggctatc agggaccagg aagagacgct ggcgaaactc
     1801 cgggaaaaaa catggggtgt gacgtatcac gagggcagga acgggaaatt tctggtgctg
     1861 ccgtccgggg tgaaggggga aaacaactgg acggtggaga agaagaacgc agtaagactg
     1921 gtcagggagt aagtgagtat gacagagctg gaaatgcact tactgaacgc cttagagcaa
     1981 ttgcagcagg actatacgca gcggctgaaa gactgggaga gcgccttcac ggatttgcag
     2041 catatgttcg agctgacacg acaggagaac gggcaactga gggaacagtg cgacgacttg
     2101 agtcagcagg tgcagagatt agccgggcag gtggaccgct tgagtcggtt atttatcacg
     2161 aacagcagat aagggatgaa caggaaaggc aggcacgcct ggaacgggag cgtgcgcggt
     2221 cctggcatga agagcaggaa cgggaagtac gggtatggcg tggtccgtcg ctggggcggt
     2281 gaatgtgtaa aatagcagtg cctagctgag tgtggaggtg attttgtccg aacaagaaga
     2341 aagagaacag aaacgacgtg agtacagggc gttgctggag ggattctggc ctgatgcgtt
     2401 taatttcagc cgtccccgtc cgctgaaagt gggtattctg gctgagctga ttgcagatgc
     2461 agagcagagg ggcttaccgt ttgattcgga actcctgaca tcggcaataa aattttatac
     2521 ccgtcgttat gtgtaccagc gtgctttaac tacgatgaaa gaacgcacag gtcttgatgg
     2581 tcagattgcc ggggaggtca cgccaaaaca gcgggagtat tccctgaaac tgatcaggcg
     2641 tttcgctgcg aaggataaag cggaaaaaag agcacgtcag gaagaaaaag ccgcgaaaca
     2701 gtcgggaatt aatccgggtg cataggtatt catcaggtta aaatttatca tgtgcgatcg
     2761 cggcagtttt tcgggtggtt tgttgccatt tttacctgtc tgttgccgtg atcgcgcctg
     2821 gag
//
LOCUS       DAZGAN020000251         2774 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGAN020000251 DAZGAN020000000
VERSION     DAZGAN020000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.253"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            188..397
                     /locus_tag="ACHKYO_RS25465"
                     /old_locus_tag="pgaptmp_005093"
     CDS             188..397
                     /locus_tag="ACHKYO_RS25465"
                     /old_locus_tag="pgaptmp_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431746.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            514..>2774
                     /locus_tag="ACHKYO_RS25470"
                     /old_locus_tag="pgaptmp_005094"
     CDS             514..>2774
                     /locus_tag="ACHKYO_RS25470"
                     /old_locus_tag="pgaptmp_005094"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
                     /protein_id="HGU4431747.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAVDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGV"
BASE COUNT          796 a          567 c          692 g          719 t
ORIGIN      
        1 atcaatgtca tgaaaataag gtgaaaaatg ataatgccga cttatttatc atttatatat
       61 tgtcgctgtt tatctattta aattttttgt gcttttgttt ttgtctttac cctccgtttt
      121 ggtaatctga gctcataggt tatttgacca ggaatgtttg ttaatccaat gaatggatgg
      181 ttaatctatg aaactgaaaa aactccccgg gtttagtttg ggacttattg ctctggcggt
      241 gggtaatgca tatgctgaac agtttattca gcacgatgaa tataaacatg ctgtggcaat
      301 aaaagatgga aaagtaaaag atattgataa tgcgctagtt ataacaacgg gtaatggaag
      361 ttacgggtat ccgttgcagg agagggtagc aagttaacca ttaacaatgg cgtcattgta
      421 acaacgggcg gtttatatcc ttctgacaat agtcctctaa gcacagcatt aacagctagc
      481 gcaatcgtaa gtgaatttgg cggtgctgtt attttgcagg gtaaaaacac gattgtcata
      541 acgggggatt actcaattgg tttgctaagt caaacgagtg gaaacctgaa tactgacacc
      601 ataataagag tcaactctga cggttcggtt accccctctt tttctgacgg agatgataca
      661 tttattgtta ctgcgggtaa tcacgcagtc ggcgttcttg cgtgtgcatc tcccggaagc
      721 gcgcgtgcgt gtgtatcttc tcttgatgaa gaaagtacta ccgatacagg aagtaatgaa
      781 aataatgcca tagcaaaact ggatatggca aaaggggaga tcacaaccca cggaacagaa
      841 agttatgccg cgtacgctaa tggcactgtc gtcaaggctg ttgacacgct tgattatacc
      901 aatgccagcg ttacattaac cgatgttgat atcaccacgc atggggacaa tgctcatgcc
      961 atcgctgccc gccaggggac agtttcattt aaccagggag aaatttatac aacgggtcct
     1021 gacgccgcga ctgctaaaat ttataatggc ggcacggtga cgctgaaaaa tacatctgca
     1081 gtcgcgcatc aggggtctgg aattgtgctg gagtcttcca taaatggtca ggaagcaacg
     1141 gtagatattt tatctggtag ctcactacgg tcagcaaatg aaatcctcta ccataaaaat
     1201 gagacgagta acgtgaccat tacggatagt gaggtatcat cggctgcaga tgtttttatc
     1261 aataatatta aaggtcattt gaccgtcgat gcaactaatt caaaaataac gggttcagct
     1321 aatatttcaa cagatgttaa cactcatacc tatctgtcgc tttcagataa tagtacctgg
     1381 gatattaaag ctgactcaac ggtgagcaac ctcacggttg ataatagtac ggtctatatt
     1441 tcccgggcag atggacggga tgtcgaacca acaagattaa caataactga aaattacgtt
     1501 ggtaataatg gcgtattgca tctcagaact gaattgggtg atgataattc agctacggat
     1561 aaagtcgtta ttaatggaaa tacctctgga acaacccgag ttaaagttac caatgcagga
     1621 ggcagcggag cttacacgtt aaatgggata gagattatca gcgttgaggg ggaatcaaat
     1681 ggggaattta ttaaggattc gaggattttc gccggtgcct acgaatattc attaacccga
     1741 ggtaataccg aagcgaccaa taaaaactgg tatctgacta acttccaggc aacgagcggc
     1801 ggtgaaacaa actccggagg aagttcagcg cctactgttg cgcctacccc cgtcctgcgc
     1861 ctcgaagctg gaagttacgt cgccaacctg gcagccgcta acactctttt tgttatgcgt
     1921 ctgaacgacc gtgcgggtga aatgcgctac atcgatcctg taactgaaca ggagcgttca
     1981 agccgacttt ggctacgtca aattggcggg cataatgcct ggcgtgacag caacggacag
     2041 ttgagaacga cctcgcatcg ctacgtctcg cagttagggg ccgatctgtt aaccggtggt
     2101 tttaccgaca gtgacagttg gcgtttggga gtgatggctg gttatgcccg cgactacaac
     2161 tcaactcatt ccagcgtgtc ggattatcgt tcgaaaggga gtgtcagagg ctattgcgca
     2221 gggctgtatg ccacctggtt tgccgatgac atcagtaaaa aaggcgcata cattgacgcc
     2281 tgggcgcaat atagctggtt taaaaactcg gtgaaagggg atgaattagc ctatgaatcc
     2341 tatagtgcga aaggcgcaac cgtctcgctg gaagcgggtt acggctttgc cctgaataaa
     2401 tcctttggtc tggaagcggc gaaatacacg tggatattcc agccacaggc acaggctatc
     2461 tggatgggcg tcgatcataa tgcgcacacc gaagccaatg gctcacgtat tgagaatgac
     2521 gcaaataaca acatccagac ccgacttggt ttccgcacct ttattcgtac tcaggagaaa
     2581 aacagcggtc cgcacggtga cgactttgaa ccttttgttg aaatgaactg gatccataac
     2641 agtaaagatt ttgctgtctc aatgaacggt gtgaaagtcg aacaagatgg ggcgagtaat
     2701 ttgggggaaa ttaaacttgg cgtaaatggc aacctgaatc cagcggccag cgtctggggg
     2761 aatgtgggcg tgca
//
LOCUS       DAZGAN020000252         2754 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGAN020000252 DAZGAN020000000
VERSION     DAZGAN020000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.254"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..174
                     /locus_tag="ACHKYO_RS25475"
                     /old_locus_tag="pgaptmp_005095"
     CDS             <1..174
                     /locus_tag="ACHKYO_RS25475"
                     /old_locus_tag="pgaptmp_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HGU4431748.1"
                     /translation="AVVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVG
                     GSQVFSFAEHGLL"
     gene            243..464
                     /locus_tag="ACHKYO_RS25480"
                     /old_locus_tag="pgaptmp_005096"
     CDS             243..464
                     /locus_tag="ACHKYO_RS25480"
                     /old_locus_tag="pgaptmp_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU4431749.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     NIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            540..917
                     /gene="cbeA"
                     /locus_tag="ACHKYO_RS25485"
                     /old_locus_tag="pgaptmp_005097"
     CDS             540..917
                     /gene="cbeA"
                     /locus_tag="ACHKYO_RS25485"
                     /old_locus_tag="pgaptmp_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HGU4431750.1"
                     /translation="MSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPAITV"
     gene            964..1341
                     /locus_tag="ACHKYO_RS25490"
                     /old_locus_tag="pgaptmp_005098"
     CDS             964..1341
                     /locus_tag="ACHKYO_RS25490"
                     /old_locus_tag="pgaptmp_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4431751.1"
                     /translation="MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKHPEAKQ"
     gene            1338..1826
                     /locus_tag="ACHKYO_RS25495"
                     /old_locus_tag="pgaptmp_005099"
     CDS             1338..1826
                     /locus_tag="ACHKYO_RS25495"
                     /old_locus_tag="pgaptmp_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4431752.1"
                     /translation="MKLTLTPEADSVNVQALNMGRIVVDVDGVNLAELINVVCDNGHA
                     LRVVDESDRVSTDCTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            1838..2035
                     /locus_tag="ACHKYO_RS25500"
                     /old_locus_tag="pgaptmp_005100"
     CDS             1838..2035
                     /locus_tag="ACHKYO_RS25500"
                     /old_locus_tag="pgaptmp_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4431753.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            2120..>2754
                     /locus_tag="ACHKYO_RS25505"
                     /old_locus_tag="pgaptmp_005101"
     CDS             2120..>2754
                     /locus_tag="ACHKYO_RS25505"
                     /old_locus_tag="pgaptmp_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4431754.1"
                     /translation="MSDITVSRPEVVNGHTDVICSTSILHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQNFHCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISKANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHFITGQQADRLADLERMLHLFSG"
BASE COUNT          681 a          762 c          687 g          624 t
ORIGIN      
        1 gccgtggtgc tggcacacaa tcacccgtcc ggtgaagtca cacccagtaa ggcagaccgg
       61 cttatcaccg aacgtctggt acaggcgctg gccctggttg acatccgggt gccggaccat
      121 ctgatagtcg gtggcagcca ggttttctcc tttgcggaac acggtctgct ttaacctgtc
      181 accgtcacaa ccacatcata cctgttttcg cttttatctt ctgtcttcag aggtatcaca
      241 ctatgaaaat catcacccgt ggtgaagcca tgcgtattca ccaacaacat ccggcatccc
      301 gtctttttcc gttctgtacc ggcaaatacc gctggcacgg cagcactgaa gcgtataccg
      361 gtcgtgaagt gcagaatatt cccggtgtgc tggctgtatt tgcagaacgc cgtaaagaca
      421 gttttggccc gtatgtccgg ctgatgagcg tcaccctgaa ctgaatcagg gcattcagat
      481 gagcagaatt atcgccacca tcgaaccatt cttaactgat tttctgtgag gactttatcg
      541 tgtcagacac actccccgga acaacgcatc ccgacgacaa tcacgaccgc ccctggtggg
      601 ggctgccctg caccgtgacg ccctgtttcg gggcacgtct ggtgcaggag ggtaaccgcc
      661 tgcattacct tgccgaccgc gccggtatca gaggcctgtt cagcgacgcg gatgcgtacc
      721 acctggacca ggcctttccg ctgctgatga aacaactgga actcatgctc accggcggtg
      781 aactgaatcc ccgccatcag cataccgtca cactgtatgc gaaagggctg acctgcgaag
      841 ccgacaccct aggctcctgt ggttacgttt atctggctgt ttatccgaca ccggcagcgc
      901 ccgcaatcac cgtataaccc cggatatctc ccctttatca ccacctgtta cagagaactt
      961 aagatgaata cattacccga cactcacgta cgggaggcat cgggctgccc gtctcccgtc
     1021 accatctggc agacactgct cacccgactg ctggaccagc actatggtct cacgctgaat
     1081 gacacaccgt tcgctgatga acgtgtgatt gagcagcata ttgaggcagg gatttcactg
     1141 tgtgacgcgg tgaactttct cgttgaaaaa tacgcactgg tgcgtaccga ccagccggaa
     1201 ttcagtgcct gtacccgttc tcagttaata aacagcatcg atatcctccg ggctcgcagg
     1261 gcgaccggcc tgatgacccg cgacaattac agaacggtaa ataacattac ccaggggaag
     1321 catccggagg cgaaacaatg aaactgactc tgacgccgga agctgacagc gttaacgtgc
     1381 aggcgctgaa catgggacga attgtcgttg acgtcgatgg tgttaatctc gctgaactga
     1441 ttaacgtggt ctgcgacaat ggccacgccc ttcgtgttgt tgatgaatct gaccgggtct
     1501 caacagactg cacgccacca tttgctgccc tgaccggcat acgctgcagt accgcgcata
     1561 tcactgcaaa ggacaacgcc tggctgtact cgctgtcaca tcagacgaat gacaccggcg
     1621 aatcagaatg gattcatttc acaggtagcg gatatctgtt acgtaccgat gcgtggtcat
     1681 acccggttct gcggcttaaa cgcctggggc tgtcaaaaac gttccgccgt ctggttgtca
     1741 cacttacccg acgttatggc gtcagtctca ttcatctgga tgccagcgct gaatgcctgc
     1801 cgggtttacc cactttcgac tggtaaccag aaacaacatg aaatcattaa ccacggaaac
     1861 agcgctggat attctgattg cgtggctgca ggacaatatc gactgcgaat cgggaattat
     1921 ctttgacaac gatgaggata aaacggattc ggcagcactg ctgccctgca ttgaacaggc
     1981 cagggaagat atccgtacac tgcgccagtt gcagcttctg caccagaacc ggtgactctc
     2041 actcaccaga cttcattcca ctgacgccag cctgaacagg gctggcgttt tcatttatct
     2101 gcaaaaagga atatcgatta tgtctgatat cacagtctcc cgtccggaag ttgtcaacgg
     2161 gcatacggac gttatctgct caaccagtat cctccacatt ctggctgtac gaaagagtac
     2221 actgctgcaa atcgacacac ttatccggca actggctgaa atctcagcaa tgacagaaag
     2281 cattggcggt aaaaccgcac tggactgggc catgaaacag aattttcact gcggttgctg
     2341 gctgatggag aaacctgaaa ccgcaatgaa agccatcaca cgcaatctcg atcgcgaaat
     2401 ctggcgtgac ctgatgcaac gttcagggat gctttcctta atggatgcac aggcccgtga
     2461 tacatggtac cggtcactgg aatacgataa ttttccggaa atcagtaaag cgaacattct
     2521 gagcacattt gaacaactgc accagaataa ggatgaggtg tttgagcgag gggtgatcaa
     2581 cgtcttcagg ggactgagct ggaattacaa aaccaactca ccctgtaaat ttggcagtaa
     2641 aattatcgtc aacaacctag tgaggtggga ccggtggggg tttcatttta tcaccgggca
     2701 acaggcagat cgacttgccg atctggaaag aatgctacac ctgttcagcg gcaa
//
LOCUS       DAZGAN020000253         2736 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGAN020000253 DAZGAN020000000
VERSION     DAZGAN020000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.255"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..145
                     /locus_tag="ACHKYO_RS25510"
                     /old_locus_tag="pgaptmp_005102"
     CDS             <1..145
                     /locus_tag="ACHKYO_RS25510"
                     /old_locus_tag="pgaptmp_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HGU4431755.1"
                     /translation="DEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSNWT
                     FQE"
     gene            159..399
                     /locus_tag="ACHKYO_RS25515"
                     /old_locus_tag="pgaptmp_005103"
                     /pseudo
     CDS             159..399
                     /locus_tag="ACHKYO_RS25515"
                     /old_locus_tag="pgaptmp_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            419..750
                     /gene="sctI"
                     /locus_tag="ACHKYO_RS25520"
                     /old_locus_tag="pgaptmp_005104"
                     /pseudo
     CDS             419..750
                     /gene="sctI"
                     /locus_tag="ACHKYO_RS25520"
                     /old_locus_tag="pgaptmp_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            747..1481
                     /locus_tag="ACHKYO_RS25525"
                     /old_locus_tag="pgaptmp_005105"
                     /pseudo
     CDS             747..1481
                     /locus_tag="ACHKYO_RS25525"
                     /old_locus_tag="pgaptmp_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
     gene            1497..2078
                     /locus_tag="ACHKYO_RS25530"
                     /old_locus_tag="pgaptmp_005106"
     CDS             1497..2078
                     /locus_tag="ACHKYO_RS25530"
                     /old_locus_tag="pgaptmp_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HGU4431756.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLASTLLERITFYAKKNRDELDKISCKWCCD"
     gene            2107..2292
                     /locus_tag="ACHKYO_RS25535"
                     /old_locus_tag="pgaptmp_005107"
     CDS             2107..2292
                     /locus_tag="ACHKYO_RS25535"
                     /old_locus_tag="pgaptmp_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431757.1"
                     /translation="MVSLKESILLETSAKIQSIEVKREEKFIQGYYDGYTKGIIDVMD
                     NFIPLISLLSSELEKKE"
     gene            2295..2726
                     /locus_tag="ACHKYO_RS25540"
                     /old_locus_tag="pgaptmp_005108"
     CDS             2295..2726
                     /locus_tag="ACHKYO_RS25540"
                     /old_locus_tag="pgaptmp_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431758.1"
                     /translation="MINDLKSILLKSSEEVDVFIKIFESWRKKLPAISGPVNLFIPTR
                     FKDKYLEVESYFVDKSIWNVHITFHDDKRFVFFTDQFIAEFSPQEFVDNCEQYLINNH
                     CFSPDKVNEICEQARHYLVEKMFETHSLDMNNSVLASPEDL"
BASE COUNT          924 a          438 c          518 g          856 t
ORIGIN      
        1 tgacgaatat tataaacaat ggggtaaaca atatgtaaga tttaatgtga atttgaaaaa
       61 tcaggacaca aataatagtt catttagcta cggagataac cgattcgaga agtctcaagg
      121 tagcaactgg acatttcagg aataattgag gaggacttat ggcagattgg aatggttata
      181 ttatggatat cagcaatcaa tttgatcagg gcgttgatga tctgaaccag caagttgaaa
      241 aagcgttgga ggatttagcc aaccaatccc tccgacacga aatttcttgc cgaatatcaa
      301 agtgcattag ctgagtatac attatatcga aatgcgcaat ctaacgttgt aaaagcgtat
      361 aaagatcttg attcagcaat catacaaaac ttccgctaaa ctggataagg attttattat
      421 gtcagtaagc aatatgccac ctattgatag agctgagcaa tcaactgctc atgaaataca
      481 gcaagcgaaa gtgattgatt taaatgaccg ggttctcaat cttgacaacc cggatgataa
      541 aatgatctcc gcttttgcca actatgctgt acaaactgaa aattggcagc aaaatgcgtt
      601 acaagcatta aggtctgata aagaggggtt aactcctgaa aaattattag tcttgcagga
      661 tcatgtgctt aattataatg tcgaggtttc attggtggaa cattagcaag aaagattgtc
      721 gctgcagttg agacgttgac ccgctcatga aatatatatc attattattg tttatcctgc
      781 tgttgtgcgg ctgcaagcag caggaattat tgaaccatct tgatcaacag caagctaatg
      841 atgtccttgc tgttttacaa agacataata ttaatgctga aaagaaggat taaggcaaaa
      901 caggattttc aatatttgtc gaaccaacgg attttgcttc tgcggtggat tggctaaaaa
      961 tctacaatct accgggtaag cctgatattc aaatatcgca gatgttccct gcagatgcac
     1021 tcgtttcatc tccacgagcg gaaaaagcaa ggctatattc tgctattgag cagcggttag
     1081 agcaatctct caaaattatg gatggcatta tttcttcgag agtacatgtt agttacgatg
     1141 ttgataatgg cgatagtggt aaaactgctt taccaattca tatttctgta cttgcagtat
     1201 atgaaaaaga tatcaaccca gaaataaaaa taaatgatat caagagattt attgttaata
     1261 gctctgcaag cgtacaatat gaaaatattt ctgttgtatt gtcaaaacga agagacatta
     1321 ttgagcaagc accaacatat gaaattagcg aacctgtttt tgcttatgat aaggctatgc
     1381 cggtttctat cttacttgca ttaatatccg tagcgacttg ctggctgtta tggaagtata
     1441 gggccattct aactaattta gtcaggttga aaattaagta gacatatgag agcgttatga
     1501 atcttgccct aagaaagatt atatatgatc caattagtta tattcatcct caacgagtaa
     1561 gcttgaataa tacacctatt aacaatccgg tgcttagatc tattaccaat gaaatgattg
     1621 ttctgcagta caatctttca gttgaacatt ttaatctgaa tagttcactt atttactaca
     1681 taaacaattg gaatttattt ccattgttct gtttattcag tggttatcac ttttatcgag
     1741 aaaggtttgc agaacgcgga tttttttata aggttcctgc tgtgctaagg gattatttat
     1801 cagctatacc ggtgaaaata aatgaaaagg cacgatacaa acccggcatc gctagctatc
     1861 acaatatcat cacatgtggt ttttcaacgt tgtcacccta tataaggcaa caaccattgg
     1921 caatgcagca acgatttaat ttattatttc ctgattttgt tgatcacata caattgccgt
     1981 taccacttgc atcaactctt ttagagagaa ttacatttta tgcgaaaaaa aatagagatg
     2041 agcttgataa aatctcctgc aaatggtgtt gtgattaaaa gaaaaatttc tgatggattg
     2101 aaataaatag tttctcttaa agaaagtatc cttcttgaaa ccagcgcaaa aattcagagc
     2161 attgaggtaa agagagaaga aaaatttatt caaggttatt atgatggcta tacaaaaggc
     2221 attattgatg tgatggataa ttttataccc ttgataagct tgttgagctc agaactagaa
     2281 aaaaaagaat aaacatgata aacgatctga agagtatatt gttaaaatcc tcagaggaag
     2341 ttgatgtttt tatcaaaatt tttgaaagtt ggagaaaaaa attaccggca atatcaggtc
     2401 ctgtaaatct ctttatacct accagattca aagataagta tcttgaagta gagtcatatt
     2461 ttgtcgacaa atcaatatgg aatgtccata ttacatttca tgatgataag cgttttgtgt
     2521 tttttaccga tcaatttatt gctgaattta gtccgcagga atttgtggat aattgtgagc
     2581 aatatttaat taataatcat tgtttctccc ctgacaaggt taacgaaatc tgcgaacaag
     2641 ccagacatta tttagttgaa aaaatgtttg aaacacattc tttggatatg aataattctg
     2701 ttttggcaag ccctgaggac ttatagctcc ctggct
//
LOCUS       DAZGAN020000254         2726 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZGAN020000254 DAZGAN020000000
VERSION     DAZGAN020000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.256"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..1393)
                     /locus_tag="ACHKYO_RS25545"
                     /old_locus_tag="pgaptmp_005109"
                     /pseudo
     CDS             complement(1..1393)
                     /locus_tag="ACHKYO_RS25545"
                     /old_locus_tag="pgaptmp_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1339..1560)
                     /locus_tag="ACHKYO_RS25550"
                     /old_locus_tag="pgaptmp_005110"
     CDS             complement(1339..1560)
                     /locus_tag="ACHKYO_RS25550"
                     /old_locus_tag="pgaptmp_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431759.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(1605..>2726)
                     /locus_tag="ACHKYO_RS25555"
                     /old_locus_tag="pgaptmp_005111"
     CDS             complement(1605..>2726)
                     /locus_tag="ACHKYO_RS25555"
                     /old_locus_tag="pgaptmp_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4431760.1"
                     /translation="NVAAVASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVA
                     RRQYPDDSRLHHVLKSAVGAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQG
                     GIPALRQVPFNIRVHAQVSGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEE
                     LIRFSSPAADALVRNALAEAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNP
                     DADAEAAFGQFVAANLQPTGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGL
                     PVIVSQYVGDQLVLVNAPDIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVS
                     MFQTGSVAIRAERWINWRRRRTAAVAVITGVNYGSASGG"
BASE COUNT          562 a          886 c          690 g          588 t
ORIGIN      
        1 gcacggataa tggacagttc acgttcattc attttttcag aatccccctg aacatcgcct
       61 tcaccgcatc atgctctgtt tcagtgattg ccttattacc gtcagaggca acgaccgcct
      121 gaggcacgga agctgtttta ccggacgacg cgaacggatc ttcacgggca tcacggcggg
      181 acaacgcttc cagactgtag ttctgctgct gaagatacag tgcatcaccg ccggccaggg
      241 gcggcaggtt ctcccgttta cgggcctcat tgggcgtgag aagcgtattt ttcaccgcat
      301 cccccagcgt tttcatgcgc cgctcactgt ccattctcag cagcgtggtg acatcaaatt
      361 ccgtactctc gttttccccc gtttccagcg cctcatccag taacagttca atggactcaa
      421 tcagcgtctg caggcactga gaataatact gctgctccag cgcctccacg ttgtcactgg
      481 aaggcggttg tcccacgcca atcttgtagg ccgggacacg gaacaccgaa cagacaattt
      541 cagcggtcat cttcagttgt tccaccgtct gcgaatccac cggtgaaaac gtcgtggggt
      601 tgtattttgc cccgttgctc agaatggccg ttttccccgc attttcgcct gtatacccgc
      661 tgtcccagtt gctcttcagt tttttcgcat tttcttccgt aatactgccg gggatctcaa
      721 tcaccccgga cggcctgccg ccatttctga aaaaagacgt cgaattttcc tgaatatgat
      781 gcccctgcgt ggccgccagc ccggcggcat acaccggcgg caaccctata agcggatgaa
      841 aaaaacagtt aaaccggtcg tggatcactt cccgggcagg caccgtcacc gcctcagtga
      901 tcccgcagtt ccggtccggc gtgatgcggt agaacacctc gccgtcatcc gccaccagag
      961 gttcaacccg gttccagtcc agaatacgca gttctttgat ctgcccccgg gcattacgga
     1021 ttttcagcac caccgtattg ccgtgacgca gtttggcgtt cagccacagt tcaaaaaact
     1081 ggatgcggtt ctgctgggcg ttgggacgac gacagaggcg ggcaatatcc ccccggcgcg
     1141 tttccctgcg tatcccctgc gcatccgtct gcataagacg cagccgcatt ttggcgatat
     1201 cctgggatat cagcgaaata catgcaaaca ccgcatgaaa ggagaggacg gcttcaggat
     1261 cggctttcac gccctgctgc caggcgccgg aaaagggctc agccaccgcc tgaaacaggc
     1321 tggtccagcc cgcctctctt acgtcacgtc ctgatttctg gttttttcgg gttcgccgta
     1381 aaaggttcca cattcgccat gctccgcatc acgtttcttt ttctgacctg ccggacgtcg
     1441 caccgtgatg tactccgcct ttcccaggcg aaccagcacc tccgcacacg gctgtgccac
     1501 atcacggata tccccggccc gggcatcatg cgtgccctgc agatatcgga tctttgccat
     1561 aacctgttac gggaggcaca cgcctcccgt cctccttatc agactcagcc gccggacgca
     1621 ctgccatagt tcactccggt gatcaccgcc accgccgcgg tacggcgacg acgccagttg
     1681 atccagcgct ccgcacggat ggccacgctg cctgtctgga acatggaaac cagctccacc
     1741 ggcgacggcg tggtactgtc gccggtcggc tcagactgca tttccagtga tgcctcgcgg
     1801 gacatatcca ctgccacgcc gccgtcatcc gccagataaa tatccggggc attcaccagc
     1861 accagctggt cacccacgta ctgagagaca atcaccggca gcccctggaa ggagccaccc
     1921 agcagggtca tgtccgggta ttctttctga cccagcgcat ttttacgcat ggacagtgcc
     1981 agggcatttg tgctggacat cagccagacc gcaccggtgg gctgcaggtt tgctgccaca
     2041 aactgtccaa acgcagcctc tgcatccgca tccgggttac cgcttgatgc cgtgcccttc
     2101 acatcatggg tgatggacgc cggggagaca tctgccaccg ccgctttttt cgggtccaca
     2161 aagtctgtat ccagacgcgc caccaccgct tccgccagcg cattacggac cagtgcatca
     2221 gcagccggac tggaaaaacg gatcaattct tccgtcagta ccgcaatggc cgacaccttc
     2281 gcatgactga aggtgatgga ttcaaaatca aacttcgtca ggggttttgc cttaccctca
     2341 cccacccagc cggcagcacc gccggacacc tgggcgtgca cacggatatt gaacggcacc
     2401 tgacgaagtg cagggatccc gccctgacca aatcgcccga taatggtctg aggacgcagg
     2461 taatcaataa agtcctgtgc gtattcctga tattcagaca ggctgcctgc ccactgcgga
     2521 tccgtggtgg tccccgcgcc cactgccgat ttcaggacat gatgcagacg actgtcatcc
     2581 ggatactgac gacgggccac ttccagggct tcagagcgga cacctttagc cgcagccagc
     2641 gatttggcaa aacgtgcgaa accaatcccc ttatccagtt tctgctccac acggatcacc
     2701 ggcgcagaag ccaccgcggc cacatt
//
LOCUS       DAZGAN020000255         2663 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGAN020000255 DAZGAN020000000
VERSION     DAZGAN020000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.257"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            144..2087
                     /locus_tag="ACHKYO_RS25560"
                     /old_locus_tag="pgaptmp_005112"
     CDS             144..2087
                     /locus_tag="ACHKYO_RS25560"
                     /old_locus_tag="pgaptmp_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431761.1"
                     /translation="MKMIFTGKVSGEKTVLTAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEGFWEECQPGETQCTAVFNVVGENGQVTEAVLTQ
                     DGPVLENFTAGQSGTLVEGINNGIIWGGIAFGAGLAAIALAAGGGGGSSRHKRATEDE
                     QKPVQPEGGQEAGKTDVSSTGSIEPESGNLSGRVTDQDGNGKADAEEAAAAVAAATEK
                     VEAAEAADKAAKDKLAELNADKLITPEEKAQLETAKQNADTLKGEADSAVKALPDTAA
                     EKGGLQGRVDALDGIQVPEVNDQDGNGKADAEEAAVAFYERALPSNVSGDLYPQPSIF
                     GDKVSSVSKNWSTLLDSNPGSYVTSQRLDSGANQYNYNGHTGSDVISITDSFGGLDRT
                     QVSRFPVGLFTGEGNDLIVTGRDYGRNTSAGYTDHSHRTDMGNGDDTLVVGVGNNDVT
                     LYVNEEGQLRATTDSYNGSTSIDYTGLNSSSSGGTISGTDIVMGAGNDTVLALGYEGN
                     SADAIINTNIDLGAGNDFIYANGEISTNNGTQVNIIGGEGFDTISLDNTTVTSAMFSG
                     FEHVDLHSTSHLILNSDDFKSQDIEGEILKISGSSGASVDVQNFDWENLSSANDGDVK
                     YFTYQSTDIPGLTLWIQEGIEVK"
     gene            2146..>2663
                     /locus_tag="ACHKYO_RS25565"
                     /old_locus_tag="pgaptmp_005113"
     CDS             2146..>2663
                     /locus_tag="ACHKYO_RS25565"
                     /old_locus_tag="pgaptmp_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431762.1"
                     /translation="MGLFNYKDYHQAQITGLISASYNIAVYANTGKVLGLGSFLVSRL
                     FAKHPDRIDYSTDVLNEGWRDITVEELRLSSEYVDSRGFYRFPSPVTGNPASGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAISNG
                     LSGEDVIVTGYSL"
BASE COUNT          788 a          461 c          720 g          694 t
ORIGIN      
        1 ataaatctga aaaaagcgga atgattaagg aggtccggtc agagaaataa attatctgta
       61 atctgattcg tttttattta atgactccct ttagcggata aaacataaga gcatactggt
      121 tttattaacc tgaggatact tatatgaaaa tgattttcac cggcaaggtc agtggagaaa
      181 aaacagtact gacagccgga gcccgtcata cagtaaaggc acaggccggg gaacagtatg
      241 gactggttga tgaggtgaca ggtctggtcc cggatggtgt ggaagcggac cgttctggtg
      301 atgatttaat tctgcgtaaa aaggaagatg atacagaaat ccggattgag ggattctggg
      361 aagaatgcca gccgggtgaa acacagtgta cagcagtatt taatgttgtg ggtgaaaatg
      421 gtcaggtaac tgaagctgtc ctgacccagg atggccctgt actggagaat ttcactgcag
      481 ggcaaagtgg cacactggtt gaaggtatca ataacgggat tatctggggg gggattgctt
      541 ttggtgcagg gctggcggct attgcactgg ctgccggagg cggtggtgga agcagtcgtc
      601 ataagcgtgc aactgaagat gagcagaagc cggttcagcc tgaaggcggt caggaggctg
      661 gaaaaacaga tgtatcatcc acaggctcaa ttgagccgga aagtggaaac ctctctggac
      721 gcgtgacgga tcaggacggc aacggcaagg cggatgcaga ggaagcggca gcagcggtgg
      781 cggcggccac ggagaaagtg gaagcggcag aagcggcgga caaggctgcg aaggacaagc
      841 ttgcagagct gaatgcagac aagctgatca cgccggaaga gaaggcgcag ctggaaacgg
      901 cgaagcagaa tgctgacacc ctgaagggtg aggcagacag tgccgtgaag gcgttgccgg
      961 atacagccgc ggagaaaggt ggcctgcagg gtcgtgtgga tgcactggat ggtatccagg
     1021 taccggaagt gaacgatcag gacggcaacg gcaaggcgga tgcagaggaa gcggcagtgg
     1081 cattttacga acgggcattg ccctctaatg tatcaggaga cttatatcct caaccgtcca
     1141 tatttggtga taaggtatct tctgtttcta agaactggtc tactttactt gatagtaatc
     1201 ctggaagtta tgttacatcc cagagattag attcaggagc taatcaatat aattacaatg
     1261 gtcatacagg ttctgatgtt atctcaatta ccgattcttt cggaggatta gatagaactc
     1321 aagtatctcg cttcccggtt ggtttattca caggagaagg gaatgattta atcgttacag
     1381 gaagagatta tggacgtaat accagtgcag gatacacgga tcactctcat cgtactgata
     1441 tggggaatgg agatgatacc ttggttgttg gtgtcggaaa taacgatgta acactatatg
     1501 tgaatgaaga aggacaactg agagcaacta ctgatagtta taatggtagt acatccattg
     1561 actatactgg gctcaattct tcatcatcag gtggaacaat atcaggaact gatattgtta
     1621 tgggggcagg aaatgatact gttctggctt tagggtatga aggtaatagt gcggatgcta
     1681 ttattaatac gaatatagac ttgggggcag gtaatgactt catttacgct aatggagaga
     1741 tttcaactaa caatggtact caggtaaata ttataggtgg agagggcttt gacacgatat
     1801 ctttggacaa tactacagta acaagtgcta tgttctctgg ttttgagcat gttgatttgc
     1861 actctacatc tcatctcatt ctgaattcag atgattttaa atctcaggat atagaggggg
     1921 agatattaaa aatctcaggt agttcaggtg catccgtgga tgtacaaaac ttcgactggg
     1981 agaatttaag tagtgccaat gatggtgatg ttaaatattt cacctatcag tccactgata
     2041 ttcctggttt aactctttgg atacaggagg gtattgaggt taaataaaag aaaatctaaa
     2101 caaacagccc cagcatgggg ctgttcttta aataaggaaa tactcatggg actctttaat
     2161 tataaagatt atcatcaagc gcaaataaca ggcctgatct ctgcgtctta taatattgca
     2221 gtatatgcca atacaggaaa ggttcttgga ttaggaagtt ttctggtatc caggctattt
     2281 gcaaagcacc cagataggat tgattattct accgatgttc ttaatgaagg ctggcgtgat
     2341 attacagtgg aagaactcag gttatcttcg gaatatgtcg attcaagagg tttttatcgc
     2401 tttcccagtc cggttacagg aaaccctgcg tcaggatttg gtcctcagtt gaaagttgtt
     2461 gggttgtttg atgctcaaca gaatctacag aaaattgcaa tatcattctg tggaacaaat
     2521 gatatctgcg atattgttga ttatacacag ctcaataatg gaaaagcgct gtctgaagca
     2581 gaaaacctgc ttcaaagtat cagagacttt gcaatcagta atggtttaag tggtgaagat
     2641 gttattgtca caggctatag cct
//
LOCUS       DAZGAN020000256         2635 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGAN020000256 DAZGAN020000000
VERSION     DAZGAN020000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.258"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            65..649
                     /locus_tag="ACHKYO_RS25570"
                     /old_locus_tag="pgaptmp_005114"
     CDS             65..649
                     /locus_tag="ACHKYO_RS25570"
                     /old_locus_tag="pgaptmp_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4431763.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            754..1494
                     /locus_tag="ACHKYO_RS25575"
                     /old_locus_tag="pgaptmp_005115"
     CDS             754..1494
                     /locus_tag="ACHKYO_RS25575"
                     /old_locus_tag="pgaptmp_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU4431764.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1529..2635
                     /gene="htrE"
                     /locus_tag="ACHKYO_RS25580"
                     /old_locus_tag="pgaptmp_005116"
                     /pseudo
     CDS             1529..2635
                     /gene="htrE"
                     /locus_tag="ACHKYO_RS25580"
                     /old_locus_tag="pgaptmp_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT          746 a          557 c          576 g          756 t
ORIGIN      
        1 gtgtcatgaa tagtcattct tcaaaccagt agtctgacta atggaatttt attcaaggat
       61 ttttatgtct aaaaaattag gttttgccct gtctggtctt atgctggcaa tggttgcagg
      121 tactgcttct gctgatatgg atggtggtca gttaaatatc agtggtctgg ttgttgataa
      181 cacctgtgaa acccgcgttg acggcggtaa taaagatggt ctgatcctgc tgcaaactgc
      241 aactgttggt gaaatcgatg ctggcgtgct gaatgacacc gttggcgcta aagccaaacc
      301 tttcagcatc accgttgact gcagcaaagc aaatccgaat ccaggtagta cagctaaaat
      361 gaccttcggt tctgttttct ttggtaatag caaaggaacg ctgaacaacg acatgtctat
      421 taacaacccg tctgacggcg tgaatatcgc tctgcacaat attgatggtt ccactatcaa
      481 acaggttcaa attaacaacc ctggcgatgt gtatactaaa gccctggatg caacgacaaa
      541 atctgctgtt tatgatttta aagcgtctta cgttcgtgcc gtcgcagacc aaacagcaac
      601 tgctggttat gtaaaaacta acactgcata caccattact tatcagtaag tattcaccaa
      661 ttaaattgat tgaatgaata tacagggaat agtgttaata atttctattt ttatattatt
      721 ccctgtttta attaactctg tcagggatgg tttatgtttt ttaacaccaa acatacaaca
      781 gctttatgct tcgtaacctg tatggctttt agttcatcgt ctattgcgga cattgtcatt
      841 tcgggtactc gcgtaatata taaaagcgat caaaaaagtg tcaacgtacg tctggaaaat
      901 aaagggaata acccgttgct tgtccagagt tggttagata ctggcgatga caacgctgag
      961 cctggcagta ttacagtccc ttttactgcc acgccgccag tatcgcgtat tgatgccaaa
     1021 cgtgggcaaa caatcaaatt aatgtacaca gccagcacct cactgcctaa agacagagag
     1081 agcgtgttct ggtttaacgt actggaagtt ccaccaaaac cagatgcaga aaaggtcgcg
     1141 aatcaaagtc tgctgcaact ggcatttcgc acacgtataa aacttttcta tcgcccggat
     1201 ggattgaagg gaaatccctc tgaagccccg ttagccctga agtggttctg gtcaggttca
     1261 gaaggtaagg cgtcattgcg agtgaccaat ccaacccctt actacgtctc ttttagcagt
     1321 ggtgatttag aagctagcgg taaacgctat ccgattgatg tgaaaatgat tgcaccattt
     1381 agtgatgagg tcatgaaagt cactggcctt aatggcaaag caagttctgc aaaagtacat
     1441 ttttacgcca ttaatgattt tggtggcgcc attgaaggta atgccagtct gtaatcaggc
     1501 aaggatataa ttccgcagga agcataacgt gactataaaa ccaactaaaa attatcataa
     1561 tcatctgacc cgtatcgcta cgttttgcgc gctgctgtat tgcaatagtg ctttctgtgc
     1621 tgaacttgtt gaatatgacc ataccttcct gatggggcag aatgcctcaa atattgatct
     1681 cagccggtat agtgaaggta accctgctat accgggtatg tacgacgtca gtgtttatgt
     1741 caacgatcaa cctattatta accaaagcat tacatttatt gaaattgaag gaaaaaaaac
     1801 gcacaagcct gtatcacact taagaattta ttgcagtttc atataaattc tcccgatata
     1861 aataacgaaa aagccgttct gcttgccagg gatgaaacac tcggcaattg ccttaatttg
     1921 acggaaatta tccctcaggc ttctgttcgt tatgacgtta acgagcaacg tctggatata
     1981 gacgttcctc aggcatgggt aatgaaaaat taccaaaact atgttgatcc atcgttatgg
     2041 gaaaatggca ttaatgcggc catgttgtca tacaacctca acggatatca tagtgaaact
     2101 cctggtcgaa gaaatgacag tatttatgct gcttttaacg gtgggatgaa tttaggtgca
     2161 tggcgactgc gtgcctcagg caactacaac tggatgaccg attctggcag taattatgat
     2221 tttaagaatc ggtatataca gcgtgatatc gcctcgctgc gttctcaact cattcttggt
     2281 gagtcttata caacgggcga aacctttgat tctgtcagta tccgcggcat tcgtttatac
     2341 agcgacagcc gcatgctgcc tccgacatta gcgagttttg cgcctatcat tcatggcgtt
     2401 gctaatacca acgccaaagt cactattacg caaggtggct ataagattta tgaaacgact
     2461 gtgccgccag gcgctttcgt cattgatgat ctgagtccgt caggttacgg cagcgatctt
     2521 attgttactg tcgaagaatc cgatggctca aagcggacat tctcgcaacc tttttcatcg
     2581 gtcgttcaaa tgttacgccc tggcgttgga cgttgggata ttagcggcgg ccagg
//
LOCUS       DAZGAN020000257         2599 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGAN020000257 DAZGAN020000000
VERSION     DAZGAN020000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.259"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(437..1426)
                     /locus_tag="ACHKYO_RS25585"
                     /old_locus_tag="pgaptmp_005117"
     CDS             complement(437..1426)
                     /locus_tag="ACHKYO_RS25585"
                     /old_locus_tag="pgaptmp_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HGU4431765.1"
                     /translation="MQNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYEHYGA
                     RMASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPR
                     IVADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVT
                     RGSDGSAHPHFHTLMMAPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRK
                     PKDGESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGAL
                     KDVLKLDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESD"
BASE COUNT          616 a          728 c          667 g          588 t
ORIGIN      
        1 aaaaaaacac accgcgccgc tagcagcacc cctaatataa aattatgttt tttatgaaaa
       61 tagtcagtac cacccctaca aagcggtgtc ggcgcagcta caacaccgcg ccgacaccgc
      121 ttttttaaat atcacaaaga gagcaagaga aactaatttg tcataactct ctatttataa
      181 agaaaaatca gcaaaaaatt gtttttgcgt ggggtgtggg catttcaggc atgagaacag
      241 actcgcgttt ttccggagta tcagcggaat tcttgattaa gaaacactcg acgcagagcg
      301 aaattttatg caacagcggc tgtactgcac agggattatt tatcagttga aacaggacag
      361 gatgtgtaat tgtaataccg tcacacgcga cgctatcaca attaccacct ggtcagggct
      421 gcgccccgac accctgctag tctgattcag ttttatcttt cgtcggtgag cggcggtact
      481 ttctgacctc ggttttccat tcgaacgcga tgcgtgaacc gtcatcgtcg ccctcggaaa
      541 tatcatctcc gatcaccatg tcggcgtcgg tttcctgatc gagtttcagc acatctttga
      601 gtgcgccgcc ggtcgccaca aagcggcgct tgtgcgtctg gcgagtcagt tcgaggaacc
      661 attcaggatc ggccaccatg tcagccggct tcgtggaata tttcagggtt tcggccactg
      721 cgccgcgcac cagttcagcg gtcgcgcagg ctaaggactc gccatccttc ggcttgcggg
      781 gtttcactgc ccggacatca acattcgggt cataatcaac ccgcaggcac tcccgccata
      841 gctcaaccca gcgttcatgc ctgacgtaac ttttcccatt tagcatgcca ggaggagcca
      901 tcatcaaggt atggaagtgc gggtgtgcgc tgccgtcgct gccgcgggta acttccgttg
      961 tgcggatcca gccctgaaca ggcaggaact ccttgcgaac cttcatacgc tgaaaagctg
     1021 cattcatccg gttcagcatc tcgcccagtt ccccaatcgc acagttgcgc acggtcaggg
     1081 tcaggaacat ccagcgggca tcgggataat ccgccacgat acgcggcagg gactggtaga
     1141 aacgggcttg ccacatcagg gagcgcctcc actggcagac aggacagtgc cggacacggc
     1201 agaaatgtgc ctcacgcagc ctcagacgcg tttctccggt ttccttgagg gtagaccacc
     1261 cgaaacgcag taaaccgcca caggaagcca tacgagcccc gtagtgctca tattcggccg
     1321 caagcaggta aatccctccc acgtcgtcag aaacgcttct gtgtgcgtcc cagggcgcat
     1381 cagaggggga ataatcagtg aggtactgga gtttagggtt ttgcatgcac accgccccat
     1441 actgggcgat ggctcgattg acgtgttatc attgccatat cacctgttgc tctcatcatg
     1501 aatccagttt ctgcaggtgg tcacaccccc gaacgatgcc cgtcgttgcg ggggtcactt
     1561 ttaacgcaac cactcccgac agaataactc caaaaccatt ttccggcaaa tcccccctca
     1621 ggccttgcgg cacaagggct ggaggcgaat ttgaatccta gagttatccc ttaagtataa
     1681 atacaaggat cacgcgcgag atttgcttga attttgagct ggattttcag taaccaaaaa
     1741 cctcccaacc ccacccactt acacccatct catccacaga ctttcacccc gattttgtga
     1801 acaacaaaaa accgtcttgc aggtggttgg cctcactccg tgacaccaac caccatcccc
     1861 agtgttttcc ccgtgccgtc gtcaatcaca aaatcggaac ccacgccaga ggcgcggggc
     1921 cccgcgattt tctgtttgac gagttcggga acggtgagcg gtacgcggaa ttttttcgcg
     1981 ggccgtgagg cgttggcggg gtgtcggggc gcagccctga ccaggtggta attgtgatag
     2041 cgtcgcgtgt gacggtatta caattacaca tcctgtcccg aaaattcccg ccgtttctcg
     2101 gttttttgag cgtcgcagac cgcacgacaa acgaaacgaa gtgaagtttg tataagtgcg
     2161 cacttcgtgc acttcgtgag attccctgac tgccagtttg tcgatcacgc ggaacggctg
     2221 cggcacaaaa acggcaaaat gatttgctgt ccggtcgcca gaatttggcg cgtcggtggc
     2281 gatggctgta cggaacggga acgaccgcgg ggtggggcgc gtggtgtaag gacttgttac
     2341 gcgcaccgct aaaacgcgct gaacgcgatt ctgccgcgaa caccgcaaaa agggatgttt
     2401 tccccagggt aaaccggaaa aatgcgtcag gaacgttttc agcgcgttgc atgactatgc
     2461 atgaaactga atggcgatcg gtttgggcgc atacagtgcc catgagacat attttcggac
     2521 attttcaatt cgtgaatccc tgactcattg ctacatatct gaacagacac ccctagcagc
     2581 gcccccaggg gcgtcctgt
//
LOCUS       DAZGAN020000258         2579 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGAN020000258 DAZGAN020000000
VERSION     DAZGAN020000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.260"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            217..564
                     /locus_tag="ACHKYO_RS25590"
                     /old_locus_tag="pgaptmp_005118"
     CDS             217..564
                     /locus_tag="ACHKYO_RS25590"
                     /old_locus_tag="pgaptmp_005118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4431766.1"
                     /translation="MPGQPIPSELKEKGLIWLLPPYCWSHRQIARKLKISASVVSKWR
                     YELVEQGLLPENEKITSVDVEDWTPERRFSVVLETATLSEMELADYYRRMGLFVEQVK
                     EWRAISIQAHEKN"
     gene            737..901
                     /locus_tag="ACHKYO_RS25595"
                     /old_locus_tag="pgaptmp_005119"
     CDS             737..901
                     /locus_tag="ACHKYO_RS25595"
                     /old_locus_tag="pgaptmp_005119"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4431767.1"
                     /translation="MERRKIIALVREAVSNGARKSQACRCIGISIRTLQRWTDGNVIT
                     TDVSVNGAPY"
     gene            complement(905..1093)
                     /locus_tag="ACHKYO_RS25600"
                     /old_locus_tag="pgaptmp_005120"
                     /pseudo
     CDS             complement(905..1093)
                     /locus_tag="ACHKYO_RS25600"
                     /old_locus_tag="pgaptmp_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1154..2278
                     /locus_tag="ACHKYO_RS25605"
                     /old_locus_tag="pgaptmp_005121"
                     /pseudo
     CDS             1154..2278
                     /locus_tag="ACHKYO_RS25605"
                     /old_locus_tag="pgaptmp_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
     gene            complement(2275..>2579)
                     /locus_tag="ACHKYO_RS25610"
                     /old_locus_tag="pgaptmp_005122"
     CDS             complement(2275..>2579)
                     /locus_tag="ACHKYO_RS25610"
                     /old_locus_tag="pgaptmp_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HGU4431768.1"
                     /translation="HHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWG
                     TLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          715 a          570 c          629 g          665 t
ORIGIN      
        1 aggcggtatg ttcatcttga gcaattagaa tataccgaat taagaaatgc tttagcttgt
       61 gcattgtcaa cttgttataa tagaaaatat ctcattaaag ttgcaaaagg aaaatattta
      121 cctgttgacg actaggtgcg tattaccgtg aaggagatcg gtgagtaacc ccctgtgtca
      181 gcatagttgt cgcctcaatt ttttaaggag ataacaatgc caggacaacc aataccaagt
      241 gagctcaaag agaaaggact gatatggctt ttaccaccat actgctggtc acatcgtcag
      301 atagcccgta agctaaagat cagtgcctca gttgtatcaa aatggagata tgagttagtg
      361 gagcaagggc ttttacctga aaatgaaaag attacctccg ttgatgttga agactggaca
      421 ccggagcgtc gattctcagt tgttcttgag acggctacgc tgtcagaaat ggagcttgct
      481 gattattacc gacgcatggg attgtttgtt gaacaggtta aagaatggcg ggctatctca
      541 attcaggcgc atgaaaaaaa ttgacggaaa atattcgaac agacagggca ttgcgtgacg
      601 caaaaaataa gttacgagaa ctgtagaaag agcttaagcg taaagataaa gctctggcgg
      661 aagctgcggc cctgcttata ttgagggaaa agttcaatgc cctctgggac aacagcgagg
      721 aagactgacc cctctcctgg agcggcgtaa aattattgcc cttgtccgcg aggccgtctc
      781 caacggcgcc cggaaaagtc aggcttgtag gtgtatcggt atttcaattc ggacacttca
      841 gcgctggact gatggcaacg ttattaccac ggatgtaagc gtcaacggag caccgtattg
      901 acgcttattt attggtgagt actacgttcc atggcaggag ttcatcaact cggttggaag
      961 gccattccgg cagtacgctc aggataaggc gcagatacgc ttccggatcg ataccgttca
     1021 gacggcaggt gccgatcagc ccgtacaaca gtgctccacg ctcgccgccg tgatcgctgc
     1081 caaagaacat aaagatggta tgacctttat atactcacta tagcattggt gggatattcc
     1141 agaggaggtc attatgcatt cggaaaatat tgcagcttac atcgggctgg atgtacataa
     1201 agaaacgttg gcggttgcta ttgctgcacc tgaacggctt ggcgaagttc gttattatgg
     1261 tacgattaac aacgaagccc aggctgtacg tcgattattt cagaaattgc agggacttta
     1321 cggaaacatt ctttcctgct atgaggctgg cccctgtggt tttgggctat atcatcaact
     1381 cacagcgatg aatataaaat gccaggttat tgccccttcc cggataccaa aatcaccgac
     1441 agacagaatc aaaaatgatc atcgggacgc aatttctctt gccaggttac tgcgggctgg
     1501 tgaacttacc cctgtctgga tccctgacct gactcatgaa gctatgcggg atctaatcag
     1561 agcccgtgca gcagctaaac gagacagcag agttgcccga cagcgaattc tgagcatgct
     1621 attgcgaaca gacaaacgtt atgcagggaa gcactggacc ggcaaacatc gaacctggtt
     1681 agccaatcaa tcatttagtc agccatctca acagattgct tttcaacact attgtcagag
     1741 tctggagcag attgaagatc gcatacttta actcgaccag gaaatatccc gcctgttacc
     1801 tgagtggtct ctgtgcaatc tggtctgcca gttacaggcc cttaaaggtg ttggtcagct
     1861 aaccgcaata accctggttg ctgaactggg tgatttttca cgattttcca gtcccaaaca
     1921 actcatggct tttctgggac tggttccagg ggaatattcc agtggaaaca gcattcgtcc
     1981 caggggaata acaaaagttg gaaacagcga actgagacgt ctgctttacg aagccgcctg
     2041 gtcttatcgt acacctgcaa aagttggtgc atggcttata tattaccgac cggattctgt
     2101 aacacaatat tccaaagata ttgcatggaa agctcaacaa cgattgtgtt ctcgttaccg
     2161 aagtctgaca gcaaaaggga aaaaatcaca agtagccatt acggcggtgg ctcgtgagtt
     2221 gactggattt atgaggtgta ctggcaatag cggacactac catttgttct ttttttaagc
     2281 agccatctga tgatattttt ccctgaaggc tgccggggag atattcccca gacgagagtg
     2341 acgacgctga cgattgtaga aaatctcaat gtattcccgt attactgaga tggcttcatc
     2401 ccggttatta aaacgatagt ggctcaggct ctcatttttc agcgttcccc agaagctttc
     2461 catcggagcg ttgtcgtaac agttaccttt acgcgacatt gatgttttca gaccaaactg
     2521 ctcctgtatg acccggtaat cgtatgcgca gtactgtgaa cctcgatcag agtggtgga
//
LOCUS       DAZGAN020000259         2557 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZGAN020000259 DAZGAN020000000
VERSION     DAZGAN020000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.261"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2556
                     /locus_tag="ACHKYO_RS25615"
                     /old_locus_tag="pgaptmp_005123"
     rRNA            <1..2556
                     /locus_tag="ACHKYO_RS25615"
                     /old_locus_tag="pgaptmp_005123"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          664 a          561 c          808 g          524 t
ORIGIN      
        1 gtacacaaaa atgcacatgc tgtgagctcg atgagtaggg cgggacacgt ggtatcctgt
       61 ctgaatatgg ggggaccatc ctccaaggct aaatactcct gactgaccga tagtgaacca
      121 gtaccgtgag ggaaaggcga aaagaacccc ggcgagggga gtgaaaaaga acctgaaacc
      181 gtgtacgtac aagcagtggg agcacgctta ggcgtgtgac tgcgtacctt ttgtataatg
      241 ggtcagcgac ttatattctg tagcaaggtt aaccgaatag gggagccgaa gggaaaccga
      301 gtcttaactg ggcgttaagt tgcagggtat agacccgaaa cccggtgatc tagccatggg
      361 caggttgaag gttgggtaac actaactgga ggaccgaacc gactaatgtt gaaaaattag
      421 cggatgactt gtggctgggg gtgaaaggcc aatcaaaccg ggagatagct ggttctcccc
      481 gaaagctatt taggtagcgc ctcgtgaatt catctccggg ggtagagcac tgtttcggca
      541 agggggtcat cccgacttac caacccgatg caaactgcga ataccggaga atgttatcac
      601 gggagacaca cggcgggtgc taacgtccgt cgtgaagagg gaaacaaccc agaccgccag
      661 ctaaggtccc aaagtcatgg ttaagtggga aacgatgtgg gaaggcccag acagccagga
      721 tgttggctta gaagcagcca tcatttaaag aaagcgtaat agctcactgg tcgagtcggc
      781 ctgcgcggaa gatgtaacgg ggctaaacca tgcaccgaag ctgcggcagc gacactgtgt
      841 gttgttgggt aggggagcgt tctgtaagcc tgcgaaggtg tgctgtgagg catgctggag
      901 gtatcagaag tgcgaatgct gacataagta acgataaagc gggtgaaaag cccgctcgcc
      961 ggaagaccaa gggttcctgt ccaacgttaa tcggggcagg gtgagtcgac ccctaaggcg
     1021 aggccgaaag gcgtagtcga tgggaaacag gttaatattc ctgtacttgg tgttactgcg
     1081 aaggggggac ggagaaggct atgttggccg ggcgacggtt gtcccggttt aagcgtgtag
     1141 gctggttttc caggcaaatc cggaaaatca aggctgaggc gtgatgacga ggcactacgg
     1201 tgctgaagca acaaatgccc tgcttccagg aaaagcctct aagcatcagg taacatcaaa
     1261 tcgtacccca aaccgacaca ggtggtcagg tagagaatac caaggcgctt gagagaactc
     1321 gggtgaagga actaggcaaa atggtgccgt aacttcggga gaaggcacgc tgatatgtag
     1381 gtgaagtccc tcgcggatgg agctgaaatc agtcgaagat accagctggc tgcaactgtt
     1441 tattaaaaac acagcactgt gcaaacacga aagtggacgt atacggtgtg acgcctgccc
     1501 ggtgccggaa ggttaattga tggggtcagc gcaagcgaag ctcttgatcg aagccccggt
     1561 aaacggcggc cgtaactata acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc
     1621 gacctgcacg aatggcgtaa tgatggccag gctgtctcca cccgagactc agtgaaattg
     1681 aactcgctgt gaagatgcag tgtacccgcg gcaagacgga aagaccccgt gaacctttac
     1741 tatagcttga cactgaacat tgagccttga tgtgtaggat aggtgggagg ctttgaagtg
     1801 tggacgccag tctgcatgga gccgaccttg aaataccacc ctttaatgtt tgatgttcta
     1861 acgttgaccc gtgatccggg ttgcggacag tgtctggtgg gtagtttgac tggggcggtc
     1921 tcctcctaaa gagtaacgga ggagcacgaa ggttggctaa tcctggtcgg acatcaggag
     1981 gttagtgcaa tggcataagc cagcttgact gcgagcgtga cggcgcgagc aggtgcgaaa
     2041 gcaggtcata gtgatccggt ggttctgaat ggaagggcca tcgctcaacg gataaaaggt
     2101 actccgggga taacaggctg ataccgccca agagttcata tcgacggcgg tgtttggcac
     2161 ctcgatgtcg gctcatcaca tcctggggct gaagtaggtc ccaagggtat ggctgttcgc
     2221 catttaaagt ggtacgcgag ctgggtttag aacgtcgtga gacagttcgg tccctatctg
     2281 ccgtgggcgc tggagaactg aggggggctg ctcctagtac gagaggaccg gagtggacgc
     2341 atcactggtg ttcgggttgt catgccaatg gcactgcccg gtagctaaat gcggaagaga
     2401 taagtgctga aagcatctaa gcacgaaact tgccccgaga tgagttctcc ctgactcctt
     2461 gagagtcctg aaggaacgtt gaagacgacg acgttgatag gccgggtgtg taagcgcagc
     2521 gatgcgttga gctaaccggt actaatgaac cgtgagg
//
LOCUS       DAZGAN020000260         2501 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGAN020000260 DAZGAN020000000
VERSION     DAZGAN020000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.262"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2051
                     /locus_tag="ACHKYO_RS25620"
                     /old_locus_tag="pgaptmp_005124"
     CDS             <1..2051
                     /locus_tag="ACHKYO_RS25620"
                     /old_locus_tag="pgaptmp_005124"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HGU4431769.1"
                     /translation="KSRKSVKSVEPEITELANSWLKSYRLDYKLQNASLNGEIDNALD
                     AYFSKNGGAGGNCPDVKLLVKDKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARKE
                     WNFKNINGYAVNGAVHYANALLQFTNYPDIIAIGMTGWRDDGTGELHHEIGVWYVSKN
                     NLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRLN
                     NDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFRK
                     IRNFLRLKAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYKI
                     GLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATGS
                     AGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILMG
                     DGSSNILNKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDK
                     GYAAVIIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIP
                     HNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTE
                     DEYFEGTIDPDSGEDWNQTRPVDARPTLEDFKKTVGDYLAWEVSQLLKKQGENNFAGK
                     "
     gene            2120..>2501
                     /locus_tag="ACHKYO_RS25625"
                     /old_locus_tag="pgaptmp_005125"
     CDS             2120..>2501
                     /locus_tag="ACHKYO_RS25625"
                     /old_locus_tag="pgaptmp_005125"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU4431770.1"
                     /translation="MSTGSLISSGRLKPGKIKRVSAKSDQNGIIGEFDTEFMDDARHY
                     ENFISVNFFGDAFYHPYCASVEMKVHVLTLKSSKSDFNAKRGLYVASMINKALKGRFN
                     YGQQLSSSKLRDGEYFISLPVKNNE"
BASE COUNT          776 a          457 c          605 g          663 t
ORIGIN      
        1 gtaaatcaag aaagtctgta aaatctgttg agccggaaat taccgaactg gcgaactcat
       61 ggcttaaatc ttacaggctt gattacaagt tgcagaatgc atcacttaac ggagagatcg
      121 ataacgcact ggacgcttat ttttcgaaaa atggcggcgc aggcggtaat tgtcccgacg
      181 taaaactcct tgttaaggac aaatacggca agcagtatcc cgttctcatc gagtataaag
      241 gctacaagga caggcttatc agacttggca gcaacggtat tattgagaac aaagatgcca
      301 gaaaagaatg gaattttaaa aatattaatg gatacgcggt gaatggcgca gtccattatg
      361 ccaacgcact tcttcagttc accaattacc ctgatattat tgctattggt atgaccggct
      421 ggcgcgatga tggtacaggc gaactgcatc atgaaatcgg cgtgtggtac gtatcaaaga
      481 ataatttagg agccggacag aaggttggtg aattcaccga cttatcgttc cttgctgaca
      541 aaaatgttga tggatttctc aacaagataa agctgcttaa tcttcctccc gaagaactgg
      601 aaaaaatcaa ggccagcaaa gaggaagaga tagacacgcg actttcccgt ctgaataacg
      661 atatttacca gaatgaaaag gggcttggtg aaagcgatcg tgtttacctt gttgtggcta
      721 ccgttattgc aacgttgggg attccgggaa agttagcacc gctggataaa aaagaactga
      781 cctcttcaac ggaggaggac ttgcgtgatg gtgatattat tttcaggaaa ataaggaatt
      841 ttttaaggct gaaagctgtt cctgaaacaa aaagagaaat gattttgcgc tcgttgcaaa
      901 atacgctgtg gactgaaaac attaacaaac cagtcaacgg tgaaagccag ttaaagcgcg
      961 tgtttgttaa agttgttgat gatcttgggg agtattacaa aattggactg accaccgatt
     1021 ttacgggcaa actgtttaat gagatgtatc gctggcttgg atttacgcag gacaagctca
     1081 atgacgtcgt tctgacacca ccttatgttg ccacattgct ggcacgactc gccagagtaa
     1141 acaaagattc ctatgtgtgg gattttgcta caggttctgc gggattgctt gtggctgcaa
     1201 tgaatgaaat gctcattgac gccagagaaa atattcactc accgaacgaa ctacaactta
     1261 aggaagcgca aatcaaggct gaacagcttc ttggccttga ggtgttatcc agtatttata
     1321 tgctggccat cctgaatatg attttgatgg gggatggtag ctcgaatatc ctcaataaag
     1381 actcgctggc cgattttgac ggcaagtatg gatttgggaa aacaggagaa aaattccctg
     1441 cggacgcatt tattctcaat cctccgtatt cagctaaagg taacggcatg attttcgtgc
     1501 agaaggcgct gtcgatgatg gataaaggct atgctgcagt gattatccag agttcagctg
     1561 gcacaggaaa ggctacggag tacaacaaaa aaatactcaa ggaaaatacc ttgctggcaa
     1621 gcatcaaaat gcctgcggat ctgtttattg gtaaatcaag tgttcagacg tacatttacg
     1681 tttttcaggt aaaaattccg cataacgcga aacaggctgt taagtttatc gacttttcca
     1741 atgacggcta cgctcgctct aatcgcaaaa aggcaagaaa caatcttgtt gatgccgatc
     1801 gtgcaaaaga gcgttatcag gaggttgtgg atctggttca cttcgggaaa ggttgcctta
     1861 atatctttac ggaggatgaa tattttgagg gaacaatcga tcctgatagt ggtgaggact
     1921 ggaatcagac caggccagta gatgctaggc caacgctgga ggatttcaaa aagacggtag
     1981 gtgattacct tgcatgggag gtctcgcaat tactgaaaaa acaaggtgaa aacaattttg
     2041 cgggaaagta aattccccgc tcagcgataa attaagaagc gttgagtggg gagagttcag
     2101 gattggcgat ttgttctatg tgagcacagg ctcgcttatc tcctcaggaa gattaaaacc
     2161 aggcaaaatc aaacgtgttt cagcaaagag cgatcaaaac ggtatcatcg gagagtttga
     2221 taccgagttt atggatgatg caaggcatta tgaaaatttt ataagcgtta atttttttgg
     2281 agatgctttt tatcatcctt attgtgccag cgtagaaatg aaggtgcatg ttcttacatt
     2341 aaaatcaagt aagtccgact ttaatgcaaa gcgcggactg tatgttgcca gtatgattaa
     2401 taaagcttta aaggggcgtt ttaattatgg tcaacagtta tcgagttcca aactcagaga
     2461 tggagaatat ttcatctcat taccagtgaa aaataatgaa a
//
LOCUS       DAZGAN020000261         2491 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGAN020000261 DAZGAN020000000
VERSION     DAZGAN020000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.263"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            130..639
                     /gene="eco"
                     /locus_tag="ACHKYO_RS25630"
                     /old_locus_tag="pgaptmp_005126"
     CDS             130..639
                     /gene="eco"
                     /locus_tag="ACHKYO_RS25630"
                     /old_locus_tag="pgaptmp_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HGU4431771.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(788..2434)
                     /gene="mqo"
                     /locus_tag="ACHKYO_RS25635"
                     /old_locus_tag="pgaptmp_005127"
     CDS             complement(788..2434)
                     /gene="mqo"
                     /locus_tag="ACHKYO_RS25635"
                     /old_locus_tag="pgaptmp_005127"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HGU4431772.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          598 a          674 c          615 g          604 t
ORIGIN      
        1 tctcattaga atttaacact aaaagagcag gtaaaattgt ctgaatgttc tttaagttat
       61 tcataaagca aattaataaa tctgatgaat atgttaacct tcagcgacat catcggtgaa
      121 aacctataaa tgaagaagga aagcaaaaaa atgaagacca ttctacctgc agtattgttt
      181 gccgctttcg ctaccacttc cgcctgggcg gcagaaagcg tccagccact ggaaaaaatc
      241 gcgccttatc cacaagctga aaaagggatg aagcgtcagg tgattcagtt aaccccgcaa
      301 gaagatgaat ctaccctgaa agtagaactg ttaatcggtc agacgctgga agtcgattgc
      361 aatttgcatc gtctcggcgg gaagctggaa aacaaaacgc tggaaggctg gggctatgat
      421 tattatgtct ttgataaagt cagttccccg gtttcgacga tgatggcctg cccggatggc
      481 aagaaagaga agaaatttgt caccgcgtat ctgggcgatg ctggaatgct gcgttacaac
      541 agcaagctgc cgatcgtggt gtatacgcca gacaatgtag atgtgaagta ccgcgtctgg
      601 aaggcggaag agaaaattga caacgcggta gttcgctaaa atgctgtgaa gtgcggcacc
      661 ccgtaggccg aataaggcgg tcacgccgca tccgacatct aacgcccgag ccggtgcctg
      721 atgcgacgct ggcgcgtctt atcagcatac gccacatccg gcataccatg ccggatgtgg
      781 cacatcatta caacgcaata tccgccactt ctttttgaac gggttgcggt ttcaactgcg
      841 gttttggcgt actatctgct gcctgcggct tgtcgtattt cagccccagc acttcgctgg
      901 tgtactgcaa ctcgcgttct gttgccgcta catcaccgtt cagcttgcgt ccataagacg
      961 gaacgatcgc tttcaacgta gcctgccatt gcgggctgga aacgcgatcg ccaaatactt
     1021 tttccagcag atccaacata atcggcgcgg cggttgacgc ccctggcgat gcccccagca
     1081 gcgcggcaat ggtcccttgc tggtcactga ccacttcagt acccagacgc agtacgccac
     1141 ctttctcggc atcacgcttg ataatctgca cgcgctgccc cgcttgccac aaacgccagt
     1201 cctctttttt tgcctgcgga tagtactctt tcaacgcttc aaaacgatcc tcttcactca
     1261 acatcacctg actcaccaga tatttcacca gatcgaaatt atccagcccg acgtgcatca
     1321 tcggcatcac gttagaggtg gtggtggaac tcatcagatc ccacaatgaa ccgtttttga
     1381 ggaatttggt tgagaaggtg gcaaatggcc caaacagcac tacgcgttta ccgtccagaa
     1441 cgcgggtatc gatatgcgga accgacatcg gtggtgcgcc aacggatgct ttaccgtaaa
     1501 ccttcgccag atggtgatta accacgtccg ggttttccga aacaaggaac tgtccgccca
     1561 ccgggaaacc ggcgtagtct ttcgcttccg gaatccccga ttcctgtaat agcttcagcg
     1621 ccgcaccgcc cgcgccgata aagacaaatt tggcgcggat gttctgtgca gtgccgtttt
     1681 tcagatcggc aacggtaacg gtccaggtgt tatcgtcatt acgctttagg gcgcggactt
     1741 cgctgctgag ttgcagcgag aagttagatt tcttctgtaa ggaagcaatt aactggcggg
     1801 tgatctcgcc atagttcaca tcagtgccaa tttctgtgcg tgttgccgct actttctgtt
     1861 gtggatcgcg cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag
     1921 agtaacgcat accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga
     1981 aattaacgtt atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac
     2041 gcggagtacg cagcacgccg cgctcaacct ggtgcgccca gaactggcgg gaaatctgaa
     2101 atgcttcgtt aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg
     2161 tgtagttcag ttccatcagt gcagaatgcc cggttccggc gttattccag ccgttcgaac
     2221 tctcctgcgc gacaccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct
     2281 cgcgtaaata ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag
     2341 tttcctgctc ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg
     2401 ccatcgagaa gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc
     2461 tttattgcag gtgagatttg cgcggcatca a
//
LOCUS       DAZGAN020000262         2464 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGAN020000262 DAZGAN020000000
VERSION     DAZGAN020000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.264"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..213
                     /locus_tag="ACHKYO_RS25640"
                     /old_locus_tag="pgaptmp_005128"
     CDS             <1..213
                     /locus_tag="ACHKYO_RS25640"
                     /old_locus_tag="pgaptmp_005128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431773.1"
                     /translation="KKMMLHSILFTQILKIIVCYLINNAELIDVFVVCRLWIIGGIKI
                     RSSWLYNPLPVSDTECVQVTGSGRAR"
     gene            1242..1787
                     /locus_tag="ACHKYO_RS25645"
                     /old_locus_tag="pgaptmp_005129"
     CDS             1242..1787
                     /locus_tag="ACHKYO_RS25645"
                     /old_locus_tag="pgaptmp_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HGU4431774.1"
                     /translation="MSKSKALNINKRFSENPFIKDLIIPVKNKQVRISRLGEDNNIMI
                     NQSTGEVLGGTSVVTYKKVDSDNFIKLFTQNIKLAFELTQAGQKALFVLFWAVQNKTN
                     NDLVLLDQYTLNDFINENKGLSMSLPTFKRGLGELVKSQILAMNKRKGFYYINPNFVF
                     NGDRVAFTTVLEMNKSSHKDK"
     gene            complement(1867..2292)
                     /gene="mobA"
                     /locus_tag="ACHKYO_RS25650"
                     /old_locus_tag="pgaptmp_005130"
     CDS             complement(1867..2292)
                     /gene="mobA"
                     /locus_tag="ACHKYO_RS25650"
                     /old_locus_tag="pgaptmp_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HGU4431775.1"
                     /translation="MAKNKRSGSEVRQRTKVITLRVNNRVASEIRRRAKNEGLTISEY
                     IRTASLNNEIKQRVPSRYLDELIQLGRMQKKLFDNGKRPKDKEYLDVMHKIISLCAEM
                     KEVTQRISDIYNEMDLLKQEIKIIKRHHKNKYPGSNIFS"
BASE COUNT          726 a          486 c          502 g          750 t
ORIGIN      
        1 aaaaaaatga tgcttcattc aatattattt acacagattt taaaaattat tgtttgttat
       61 ttaataaata acgccgaatt aattgatgtc tttgttgtct gtcgtttgtg gattattggt
      121 ggaataaaga ttagatcttc ctggttatat aatcctttgc ctgttagtga tacggagtgt
      181 gtccaagtaa caggaagcgg aagagcaaga tagtagcatg tacataaaat ataaatacgt
      241 gagttattga tatcgcgttt atatattaaa aatataactt aacattgctc ttaatattta
      301 ttttgtcctg acaatgggag cattaatccc cgtgcagtgc tccgccagct gctgacaacc
      361 tcgacatata accatcgata cgatagaaca tcagggcgcg tttccggcaa gaactaacca
      421 tggggatggt ttgcgtgcca acgaacgccg gaaacgcgct tcaaacctac cgagtcgcca
      481 cggttccggc agcgccttga gcgcaaccgg aaaccgtctg gggagagccc cagcccccca
      541 ccgctgcaac atcgacggtc tatgaccacc caccaaccga gtagctgcct gtaccgtgca
      601 aatgggcttt gcagggtgct ggcgcggttc ctggcgacct gaaagcccct ggttaggggt
      661 atcggcgcgc ctggcctcct gaaaccctct gtaagcgttt ttcgcgcctt ccatgccctg
      721 gcgggcattt aaaacccgtt tcgtgcgtct gagagcttct gagagcgttt taggggctat
      781 ctggcaactc atccccgaaa gagcaaccgc ggaatcaccg acaacatgcg agaggtaatt
      841 ccgctcgccg caagtcgatg accgagcgta gcgagcgaat cgacgaggaa gcggaagaga
      901 accggaagct acattgagca cttacgcaca gaatttggcc tacgcacccg cgtccgtgca
      961 cgcgtttaat aagaattaag aagggaaatc gcgataaacc gcgccaatcc tagaatctaa
     1021 tgatcaggat attgatctaa cgtatcagaa tagagcatct aacatatcaa ttagttgata
     1081 tttaaagtat tgattgtttg ttttttgctc atcgtgagtg tgatttattt gatctttaag
     1141 gtatcaaaac tatgatacta gtcggatcgg tttattgaca tttgtagatt ttttcttgat
     1201 agtcgaggta tcagtttatt gatacctgag agtgtaaaat aatgagcaag agcaaggctt
     1261 taaacataaa caaacgattt agtgaaaatc ctttcatcaa agatttgata attcctgtaa
     1321 aaaacaaaca agtaagaata tcgcgattag gagaagataa taacataatg attaaccaat
     1381 caactggaga ggttttaggg ggaacctcgg tagtaactta taaaaaagtt gatagtgata
     1441 attttattaa attgtttacc caaaatataa aattggcttt cgagttaaca caagctggtc
     1501 aaaaagcact atttgtttta ttttgggcag tacaaaataa aacaaataat gatctagtct
     1561 tattagatca atacacgcta aatgatttta taaatgaaaa caaagggtta agcatgtccc
     1621 ttccaacatt caaacgtggg cttggggagt tggtaaaatc acaaatatta gcaatgaata
     1681 agcgaaaggg attttattat ataaatccta attttgtttt taatggcgat agagttgcat
     1741 tcacaacagt tcttgaaatg aataaatcga gccataaaga caaatgacaa tgtattaagc
     1801 ccgtcaaagg aaaatttcaa atctattggc gggttttaat attcgtaacg gctagcgccg
     1861 aagattttag ctaaatatat ttgagccagg gtatttattt ttatgatgcc gttttattat
     1921 ttttatttcc tgttttaata gatccatttc attatatata tcagaaattc gctgtgttac
     1981 ctctttcatt tcagcacaaa gtgaaattat tttatgcatc acatctaggt actctttgtc
     2041 cttcggcctc ttcccgttat caaataattt cttctgcatc cgaccaagtt gtattaattc
     2101 atctagatat ctggaaggca cgcgctgttt tatttcatta tttaaacttg ccgtcctgat
     2161 atattctgaa attgtcagtc cttcgttctt cgctcgacgt ctgatttcgc ttgcaactct
     2221 gttatttact ctaagggtga taaccttagt tctctgccgt acttctgaac cactacgttt
     2281 atttttagcc atcgcaatga ctctccattt tgcacgtttg gggtgtcggg gcttgccctg
     2341 acaggaggta ttctcatgca agcgaatgta ttcgcggtat gacgaatata catcctgtcc
     2401 cgttttccgc tataactttt cggcgaaaat aaaataccgc gtcgtaacct agtatttctg
     2461 atac
//
LOCUS       DAZGAN020000263         2410 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGAN020000263 DAZGAN020000000
VERSION     DAZGAN020000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.265"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            309..503
                     /locus_tag="ACHKYO_RS25655"
                     /old_locus_tag="pgaptmp_005131"
     CDS             309..503
                     /locus_tag="ACHKYO_RS25655"
                     /old_locus_tag="pgaptmp_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431776.1"
                     /translation="MPENNTRKPDKSATVHIDAGTMEKIERYQQFIKDNHPGMPVPTK
                     GQITRSAVEYWYRATLGVWL"
     gene            500..2389
                     /locus_tag="ACHKYO_RS25660"
                     /old_locus_tag="pgaptmp_005132"
     CDS             500..2389
                     /locus_tag="ACHKYO_RS25660"
                     /old_locus_tag="pgaptmp_005132"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGU4431777.1"
                     /translation="MKTWFSIKAMADVVYVRIYDEIGGYGVKASALTDEINACGNASE
                     IHLRIHSPGGDIFEGLAIYNALKNHPAKKIVHIEGMAASMASFIAMCGDHIVIPENAM
                     MMIHAPRGVTAGVSGDVRRFADLMDKLGDTMAETYAGRTGRSKQEITAMMEAETWMDG
                     NECKANGFADEVIPAITAMARIESKRIGDFSNMPEKIKSMISQKTGSGEQERLNGIRE
                     LFGTFNGRYNDLAISCLADSECSVENARERLLLAMGKESTPTNKTTPANLYYAYTDNG
                     NITGDAMRQGLNARLGHERAERGNPYAMMSLFDMAQASLTHRGISTGSYSTRSQIVNA
                     AFTHSSSDFTDILAGGAEKSVLAGWEHSGETFRQWTKKGSLSNFREARRVGMNGFSTL
                     NKVPEGAEYKYITTSDRGEPIALATYGNIFSITRQAIINDDLDQLSTVPMAMGRAASR
                     TVGNLVNLVLTGNVKLSDGIALFDKKHSNLIEAGLTTPGLSAARHLMRTQKDKNGEVL
                     NIAPKFLLVPAALEDRALQMINSTAPFGADKNSGIFNPYHKLLDIIVDPRLDDISEKQ
                     WYMLSAQGTDTIEVAYLDGNDEPYLEQQEGFIVDGVAWKVRIDAGVAALDYRGMVKSG
                     GTDSL"
BASE COUNT          702 a          562 c          607 g          539 t
ORIGIN      
        1 aaatttttag tgtcgaatca ggttgttttt tgttcatcac cggaacgttc ccgaaaacat
       61 ttacaaaaaa caggcgcaaa aaaagcgccc ccgattgctg ttaccggagg cgcttttaca
      121 cgacaaagga gtttttatcg ccaggatgac gagtcttaat actgcttcaa tggcaaaaat
      181 gcgtcaataa ctttgccttt ctgagaataa tcagaaaaat cataatctga ttttcaggta
      241 gaaaatgatt tatctattac ttttatcgat caataatgat gcccgttaat caaaacggag
      301 gcggatttat gccagagaac aacaccagaa agccggataa aagtgccacg gtacacatag
      361 acgccggaac tatggagaag atcgaacgct atcagcagtt catcaaagat aatcacccgg
      421 gtatgccagt acccacgaaa ggacaaatca cacgcagcgc ggttgaatac tggtacaggg
      481 caacgttagg ggtctggcta tgaaaacatg gttttccatt aaggctatgg cagatgttgt
      541 ctatgtgcgc atttatgacg agatcggcgg gtacggtgta aaagcatcgg cacttactga
      601 cgagatcaac gcgtgtggta atgcgtctga aatccatctt cgcatccatt cacctggtgg
      661 cgacatcttt gaagggctgg ctatctataa cgccctgaaa aatcatccgg caaagaaaat
      721 tgtacacatt gaaggcatgg cggcttctat ggcctcgttt attgccatgt gtggcgatca
      781 catcgttata cctgaaaacg cgatgatgat gatacatgcc ccccgtggtg ttactgccgg
      841 agtgtcgggc gacgttcgcc gctttgctga cctgatggac aagctgggcg acacgatggc
      901 ggaaacctac gccggaagaa cgggcaggag caaacaggaa atcaccgcca tgatggaggc
      961 ggaaacctgg atggatggca atgagtgtaa ggctaacggc ttcgcagatg aggttatacc
     1021 cgcgattaca gcaatggccc gaattgaatc aaaacgaatc ggagattttt caaatatgcc
     1081 ggaaaaaatt aaaagcatga tcagccagaa aactggcagt ggcgaacagg aacgacttaa
     1141 cggcatccgt gaattgtttg gcacgttcaa cggaagatat aacgacctgg ctataagttg
     1201 tcttgctgat tcagaatgta gcgttgagaa tgcacgcgaa cgccttttac tcgctatggg
     1261 taaagaatca acgccaacaa acaaaaccac ccccgcaaat ctttactacg cgtacacgga
     1321 taacggcaac ataaccggcg atgcaatgcg ccaggggctt aatgcgcgtc ttggtcacga
     1381 acgggccgaa cgcggtaatc cttacgccat gatgagcctt ttcgatatgg cacaggcatc
     1441 attaacccat cgtggtataa gcacgggcag ctacagcaca cgctcgcaga tagtaaacgc
     1501 ggcattcacc cacagcagca gcgattttac cgatatcctt gctggtggcg ctgaaaaatc
     1561 agtgcttgca ggctgggagc acagcggcga aacattccgc cagtggacga aaaaaggttc
     1621 cctttcaaac ttccgggaag cccgccgcgt tggtatgaat ggcttctcaa cgttaaacaa
     1681 agtgccggaa ggggcagaat ataaatacat caccaccagc gatcgcggtg aacccatcgc
     1741 gctggctact tacgggaata ttttcagcat tacccgccag gcgataatca atgatgacct
     1801 tgatcagtta tcaacggtgc caatggctat gggccgtgct gcatcaagaa cggtgggaaa
     1861 tctggttaat ctggtgctta caggcaacgt aaaactttct gacggaatag cgctgtttga
     1921 caaaaaacac agcaacctga ttgaagcagg actgacaaca ccgggactta gtgcagcacg
     1981 tcacctgatg cgcacacaga aggacaaaaa tggcgaagtg ctgaatattg cgcctaaatt
     2041 ccttttagtt ccggcagcac tggaagatcg cgcgttgcag atgattaact caaccgcacc
     2101 tttcggggct gataaaaaca gcgggatctt taacccgtat cacaagctac ttgatatcat
     2161 cgtcgatccc cgccttgatg atatcagcga aaaacaatgg tacatgcttt ccgcacaggg
     2221 aacggacaca atcgaggtgg cttatcttga tggcaatgac gagccttacc ttgaacagca
     2281 ggaaggtttt atcgttgacg gcgtggcctg gaaagtccgt attgatgcag gtgtggcagc
     2341 tctggattat cgcggtatgg tcaaatcagg cgggacagat tcactatgac aacaaggcgg
     2401 caccagccgc
//
LOCUS       DAZGAN020000264         2409 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGAN020000264 DAZGAN020000000
VERSION     DAZGAN020000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.266"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..944)
                     /locus_tag="ACHKYO_RS25665"
                     /old_locus_tag="pgaptmp_005133"
     CDS             complement(<1..944)
                     /locus_tag="ACHKYO_RS25665"
                     /old_locus_tag="pgaptmp_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431778.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPDHSAVLAEDCHFRFGVITPERQ
                     ISGVEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPVAQDNYGRIYYTDGKFPKVTAA
                     EIATKGEGNFPAASYRLGIPAPTTAPVCTVQRGEAISDDNPNDDETRFYTETFVSAYG
                     EEGPPGPESLEVTVGIPDTPVQLTLSPVPLQDANINRRRIYRSVSGGGEADFLLVAEL
                     EASVLSYTDNIPAKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSG"
     gene            complement(1125..1250)
                     /locus_tag="ACHKYO_RS25670"
                     /old_locus_tag="pgaptmp_005134"
     CDS             complement(1125..1250)
                     /locus_tag="ACHKYO_RS25670"
                     /old_locus_tag="pgaptmp_005134"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431779.1"
                     /translation="MKQANLKYVKKRYTAGAMCFMLFVWLGAVVTLCAVAAVMMQ"
     gene            complement(1294..1767)
                     /locus_tag="ACHKYO_RS25675"
                     /old_locus_tag="pgaptmp_005135"
     CDS             complement(1294..1767)
                     /locus_tag="ACHKYO_RS25675"
                     /old_locus_tag="pgaptmp_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431780.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYSVILAREFFMPENRKHTPENN
                     ESWVIICDALLGRLMRCMFQGRRYLQLDAEEMTGRLDTYRKIRNGDWVQNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYLGLGKNGLRYRSVKHLVGLA"
     gene            complement(1856..>2409)
                     /locus_tag="ACHKYO_RS25680"
                     /old_locus_tag="pgaptmp_005136"
     CDS             complement(1856..>2409)
                     /locus_tag="ACHKYO_RS25680"
                     /old_locus_tag="pgaptmp_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HGU4431781.1"
                     /translation="TTFAMDADGNITLDETAGTGERYGVKDVVAEVVEDTTVRVAEDG
                     SLTTARNTQTTKRVNEAVVDLDKSVRTNSQDIATNRKAIQSNSRQLQEHNARLNDHQR
                     QIRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQT
                     AAKAGIAASDGDVSYNVGVNFEF"
BASE COUNT          585 a          682 c          536 g          606 t
ORIGIN      
        1 ccactgaaca gataaggctc cccctttgtc gccaccacca gtgacgttcc cagcggacag
       61 atggccacaa tatcttctgc cgtcgtatga cgattcactt ccggccatgc atacggcaga
      121 tacgcttctg aaaacattac ctcattaccg gcaaatccgg cggcaatacc attcgccatc
      181 aggcaaagac ctgtcatatt ctccggcggc ggcaggtaat cccatgttgc aagagaaggt
      241 ccgaggtttt tcgccggtat gttgtccgtg taactgagca cggatgcttc aagctcagcc
      301 acgagtaaaa aatcggcttc tccgccccct gagacagaac gataaatacg acgacgattg
      361 atgtttgcgt cctgcaacgg aaccggagag agtgttaact gaaccggagt atcagggatc
      421 cccacggtga cttccagcga ttcaggtccg ggaggccctt cctcaccata cgccgataca
      481 aaggtttcgg tataaaagcg tgtttcatca tcattcgggt tatcatcaga aatcgcttcc
      541 ccgcgctgaa ctgtacaaac aggtgctgtt gtcggtgcag ggatccccag acgatatgat
      601 gcagcaggga aattaccctc tcctttagtg gcaatttcag cagccgtgac ttttggaaat
      661 tttccgtcag tgtagtaaat ccgcccgtaa ttatcctgag caacgggact gcggatcaca
      721 tccaccacat ccggccacgc aaaccaaaaa tcatcacggt aatgaaaaat tgtttttggc
      781 ttaattgtga atgttttctc aaccccggat atctgacgtt ctggtgtaat aacaccaaac
      841 cggaaatggc agtcctccgc cagtacagcg gaatgatcgg gcagcatgga tgtcacaacg
      901 cgcggcatca tcccacgcat cgtggtgata tcgatataag gcatagaaat tcctttgact
      961 gcttcaggat taaaaatcag gggatttatg gtggaattcg ctaaaatcgc gatcggaatc
     1021 actacaacag agtgttgcta tgatattctg ggcaagacgt aacaactcac taattgttat
     1081 caaacgtctt tcttctggtc ggggagcatg ttgctcccct ttctttattg catcattacg
     1141 gcagccactg cacagagcgt caccactgca cccagccaca caaaaagcat gaaacacatc
     1201 gctcctgctg tgtagcgctt ctttacgtat ttcagatttg cctgtttcat gacaatttac
     1261 ctgattgctc ttcagtaccg gataacaata cggctatgcc agtcccacca ggtgcttcac
     1321 actcctgtac ctcaggccat ttttaccaag ccccagatac agaaaggtgt tcaccgtctt
     1381 cgtatttatc cccatttcac gcgcaagatg acacggcttt gactcacggt acacataacc
     1441 aaacatcacc accatctcgg acatcgtcag aggacgcgca tacgtgttct gcacccagtc
     1501 accgttacgg attttcctgt atgtatccag acgccccgtc atctcctccg catccagctg
     1561 aagataacgg cgtccctgaa acatacagcg catcagcctg ccaagcagcg catcacagat
     1621 aatcacccat gattcattat tctccggtgt gtgcttcctg ttctccggca taaaaaattc
     1681 ccgggccagt atgacactgt acccctcaag catctgttgc aggccgtact ccagataacg
     1741 gttgcgggtc gccagaatca ccttcatcgc atctcctcac tgtctctgaa cacatgaaaa
     1801 aaacggcagg ccaaaatccc gcacctgccg tcatgcttca tgtcactgtc agcaattaaa
     1861 actcgaagtt aacgcccacg ttgtaagaaa cgtcaccgtc gcttgctgca atacccgctt
     1921 tcgctgcggt ctgctcattg aaacggtagc ccacaccgac agcaacggcc tgtttgtcac
     1981 tgtaaccacc cagggctgcc gtggcgttga atttgcccac actgtacggc tggaacagac
     2041 cggcaagtgc tgcgctctgt gccgctgcgc gtttcatctc ttcgtggttt tcacgaatct
     2101 ggcgttgatg gtcattcaga cgtgcattgt gctcctgcag ctggcggctg tttgactgaa
     2161 tcgcttttct gttggttgca atgtcctggc tgttagttct tacagattta tcaaggtcaa
     2221 ccaccgcttc atttacgcgt ttggttgtct gcgtgttacg ggctgtggtc agggaaccgt
     2281 cttctgccac acgaacggtg gtatcctcaa caacttcagc cacaacatcc ttcacgccat
     2341 agcgctcacc tgtaccggct gtctcatcca gagtaatgtt gccgtcagcg tccattgcaa
     2401 aagtggtgt
//
LOCUS       DAZGAN020000265         2366 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGAN020000265 DAZGAN020000000
VERSION     DAZGAN020000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.267"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..654
                     /locus_tag="ACHKYO_RS25685"
                     /old_locus_tag="pgaptmp_005137"
     CDS             <1..654
                     /locus_tag="ACHKYO_RS25685"
                     /old_locus_tag="pgaptmp_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4431782.1"
                     /translation="AIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDD
                     SHVVTMPELCWWLIRNDLADALPESAARKALRLPNLVVPSVTRESDLVPSVPATSIIQ
                     DKAKKVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGH
                     GQGGMGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            668..1420
                     /locus_tag="ACHKYO_RS25690"
                     /old_locus_tag="pgaptmp_005138"
     CDS             668..1420
                     /locus_tag="ACHKYO_RS25690"
                     /old_locus_tag="pgaptmp_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4431783.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAESKARCERCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGAPVYKICGRCNGNRFSRLPTTLARHRVQKLIPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEDQLRKVTR"
     gene            1569..1645
                     /locus_tag="ACHKYO_RS25695"
                     /old_locus_tag="pgaptmp_005139"
     tRNA            1569..1645
                     /locus_tag="ACHKYO_RS25695"
                     /old_locus_tag="pgaptmp_005139"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1603..1605,aa:Arg,seq:tcg)
     gene            1659..1735
                     /locus_tag="ACHKYO_RS25700"
                     /old_locus_tag="pgaptmp_005140"
     tRNA            1659..1735
                     /locus_tag="ACHKYO_RS25700"
                     /old_locus_tag="pgaptmp_005140"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1693..1695,aa:Arg,seq:tct)
     gene            1827..>2366
                     /locus_tag="ACHKYO_RS25705"
                     /old_locus_tag="pgaptmp_005141"
     CDS             1827..>2366
                     /locus_tag="ACHKYO_RS25705"
                     /old_locus_tag="pgaptmp_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030598 - rRNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-inactivating family protein"
                     /protein_id="HGU4431784.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNCIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAV"
BASE COUNT          594 a          512 c          627 g          633 t
ORIGIN      
        1 gcaatccgtc tgtgctggca ctgcgataac cagctgcgcg atcagttcac ggaacggctg
       61 gaatcaatgg cgacggataa ctgtgcccgc tgggtgttgt ctgttgtgcg tcgggatctc
      121 ggttttgatg atagtcacgt tgtgacaatg ccggaactgt gctggtggct gattcgtaat
      181 gacttggcgg atgccttacc ggaaagtgca gcccgtaagg cactgagatt accaaatctt
      241 gttgtgccgt ctgtcacccg ggaaagtgac cttgtgcctt cggttcctgc caccagcatc
      301 atccaggata aggcaaaaaa ggtgctggcg ctgaaagtgg atccggagtc gccggagtct
      361 tttatgttac gcccaaaacg tcgccgctgg gttaatgaaa agtacacgcg ctgggttaag
      421 acacagccgt gtgcatgttg tggaaagccc gctgatgatc cccaccacct gataggtcac
      481 ggtcagggtg gaatgggaac aaaagcgcat gacctctttg tgttgccttt gtgtagaaag
      541 catcacgacg agctgcatgc ggataccgtg gcattcgaag agaagtatgg ctcccagctg
      601 gagctgatat ttcgttttat cgatcgtgcg ctggcaatag gcgtactggc gtaagtggag
      661 aacgagcatg aaccttgaag ccttaccaaa atattactcc ccaaaatctc caaaattgag
      721 cgatgacgca ccggcgacag gctcgggtgg tttaacgatt acagatgtga tggctgcgca
      781 ggggatggtg cagtcgaaag caccgcttgg gtttgcctta ttcctggcaa aagttggtgt
      841 tcaggatcct cagtttgcga ttgaaggtct gctcaattac gcgatggcac tggataaccc
      901 gacattgaat aaattgagtg aagaaacccg gttacagatc atcccttacc ttgtgaattt
      961 tgcctttgct gattattccc ggtctgcgga aagtaaggct cgctgtgagc gttgtgctgg
     1021 tactggattt cataatgtat tgcgcgaagt ggtgaaacac tccagaagcg gggaatctgt
     1081 tatcaaggaa gagtgggtga aggaactatg tcagcattgc catggtaagg gagaagtcag
     1141 cacagcgtgc agagggtgta agggtaaagg tattgtcctg gatgaaaaaa gaacccggct
     1201 tcatggcgcg cctgtttata agatttgtgg gcgttgcaat ggaaaccggt ttagccgttt
     1261 accaaccaca ctggcgaggc atcgtgtcca gaagctgata ccagacctga cggattatca
     1321 gtggtacaaa ggatatgcag atgtcattga taaactggtt acaaagtgct ggcaggaaga
     1381 agcgtatgct gaagatcaat tgagaaaagt gacaagatag ataatttcgc cgaagatgtc
     1441 gacatgatgc ttgcattttt caaaaaatat ggataaagtt ttcccaacga tgggctttgt
     1501 atgtctaccg ttgataagat ttaagagccc gccgctgagc gggtttttgt tgcatctcct
     1561 gttaaatacc gccattagct catcaggata gagcgccagc cttcgaagct ggttgcgcgg
     1621 ggttcgagtc ctcgatggcg gtccattatc ggtacactgc gttgttagct cagccggaca
     1681 gagcaattgc cttctaagca atcggtcact ggttcgaatc cagtacaacg cgccataatt
     1741 atttaccagg ctcgcttttg cgggcctttt ttatatctgc gccgggtctg gtatcgtatt
     1801 acttcagcca aaaggaatac ctgtatatga agtgtatatt gttaaaatgg gtactgtgcc
     1861 tgttactggg cttttcttcg gtatcctatt cccgggaatt tacgatagac ttttcgactc
     1921 aacaaagtta tgtctcttcg ttaaattgta tacggacaga aatatcgacc ccacttgaac
     1981 atatatctca ggggaccaca tcggtatctg ttattaacca caccccaccg ggcagttatt
     2041 ttgctgtgga tatacgaggg cttgatgtct atcaggcgcg ttttgaccat cttcgtctga
     2101 ttattgagca aaataattta tatgtggccg gattcgttaa tacggcaaca aatactttct
     2161 acagattttc agattttgca catatatcag tgcccggtgt gacaactgtt tccatgacaa
     2221 cggacagcag ttataccact ctgcaacgtg tcgcagcgct ggaacgttcc ggaatgcaaa
     2281 tcagtcgtca ctcactggtt tcatcatatc tggcgttaat ggagtttagt ggaaatgcca
     2341 tgaccagaga tgcatccaga gcagtt
//
LOCUS       DAZGAN020000266         2335 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGAN020000266 DAZGAN020000000
VERSION     DAZGAN020000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.268"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..185
                     /locus_tag="ACHKYO_RS25710"
                     /old_locus_tag="pgaptmp_005142"
     CDS             <1..185
                     /locus_tag="ACHKYO_RS25710"
                     /old_locus_tag="pgaptmp_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431785.1"
                     /translation="RIGRCMGYLQVAAEIEALASGADKDAVVRETVLRDFNTPPFKTE
                     PDDWIQPGLNYLKGRI"
     gene            complement(197..511)
                     /locus_tag="ACHKYO_RS25715"
                     /old_locus_tag="pgaptmp_005143"
     CDS             complement(197..511)
                     /locus_tag="ACHKYO_RS25715"
                     /old_locus_tag="pgaptmp_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431786.1"
                     /translation="MHNHQHNYDLCLQAINERVKSECLLLLPQEHKAVKAIQAEPYGA
                     LTTATLSIISGALTPTLLLRLKDNIDDWLNEELSYLDCEWDNHYAKSQKDRLFRQLSG
                     NR"
     gene            640..1173
                     /locus_tag="ACHKYO_RS25720"
                     /old_locus_tag="pgaptmp_005144"
     CDS             640..1173
                     /locus_tag="ACHKYO_RS25720"
                     /old_locus_tag="pgaptmp_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4431787.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATQVDGKPVIPGMKLSKEKCDQVNAIERDKALTWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1394..1507
                     /locus_tag="ACHKYO_RS25725"
                     /old_locus_tag="pgaptmp_005145"
     CDS             1394..1507
                     /locus_tag="ACHKYO_RS25725"
                     /old_locus_tag="pgaptmp_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431788.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIIAIAVLIWAIRWW"
     gene            1509..1976
                     /locus_tag="ACHKYO_RS25730"
                     /old_locus_tag="pgaptmp_005146"
     CDS             1509..1976
                     /locus_tag="ACHKYO_RS25730"
                     /old_locus_tag="pgaptmp_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4431789.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENEILRADVAAGRKRLRINATCPGP
                     VREATGTARVDNATGPQLADTVTRDYFTLRERLMTMQKQLEGAQEYIRTQCIK"
     gene            2000..2224
                     /locus_tag="ACHKYO_RS25735"
                     /old_locus_tag="pgaptmp_005147"
     CDS             2000..2224
                     /locus_tag="ACHKYO_RS25735"
                     /old_locus_tag="pgaptmp_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431790.1"
                     /translation="MKKLLVTVKPFQGTIPFRVLQPGRVLVEGTFSGKCTQSYSRVFR
                     VDASNEELTVECAVNAAKCRMVSAALQPVC"
BASE COUNT          585 a          517 c          650 g          583 t
ORIGIN      
        1 agcgtattgg tcgttgcatg ggatatcttc aggtagctgc tgagattgaa gcgctggcca
       61 gtggtgctga caaggatgca gttgtgcggg agaccgttct tcgtgatttc aatactccac
      121 cctttaagac tgagccagat gattggatcc agccggggct gaattatctc aaagggcgga
      181 tataagttca ggggcgttat ctgttacctg ataattgtct gaacaggcga tctttttgtg
      241 atttggcgta atgattatcc cattcgcaat caaggtaact taattcttca tttaaccagt
      301 catctatatt gtctttcagg cgaaggagca aggttggcgt taatgctccg gatataatac
      361 tgagagttgc agtagtgaga gctccatacg gttcagcctg aatggccttt actgctttgt
      421 gttcttgcgg gaggagtaag aggcattccg attttacccg ctcatttatg gcttgcaggc
      481 ataaatcata attatgttga tggttatgca tggttaatcc tcgactggaa ttgtcagata
      541 tattttcagc cagcagataa aacgccagtg cccaccactg gcgggctgaa gacttaacat
      601 atccagggat tcggaaccga taaatcctga taaatatcca tgaacgcaaa aatcagatac
      661 ggcctgtcgg ctgccgttct ggcgctgatt gccgcagggg cgcctgcgcc tgatattctc
      721 gaccagtttc ttgacgaaaa agaaggcaac cacaccacag cataccgtga tggtgcgggt
      781 atctggacca tctgccgagg tgccacccag gtggatggta agcctgtgat tcctggcatg
      841 aagctgtcga aggaaaaatg cgaccaggtt aacgccattg agcgtgataa agcgctgacg
      901 tgggtggaga aaaacatcag agtgccgctg accgaacccc agaaagcggg gatcgcgtca
      961 ttctgtccgt acaacattgg tcccggtaag tgtttcccgt cgacgtttta cagacgaatt
     1021 aatgcaggtg atcgcagggg agcatgcgaa gcgattcgct ggtggattaa ggacggtggc
     1081 agagactgcc gtattcgctc aaataactgt tacggtcagg tatcccgtcg tgaccaggag
     1141 agcgcgctgg cgtgctgggg aatcgacaga taagcagaat attttgctga aaaatgacgt
     1201 tgaccaacgc ggacggataa cacgaaatcc tgcgaactgg caaaacctaa gtgaataaaa
     1261 gtaaaaaccc cgtttgttgg cagcaagcgg ggttttgtgt ttctgacctt ggataaggca
     1321 agggagaaat tatggtgagg gaggtgcttt ccctgtgagg aagtataaaa gattctttct
     1381 gaggttgtcc attatgaaag gcattgaagt ggagacgcca gccagtctgg atttaacaag
     1441 agcggcagct tttgccattc gtattatagc cattgctgtt ctgatctggg caatccgttg
     1501 gtggtgaaat gaaccgcgtt ctgtgtgtgg tcatcattgc tctgctggtg gcctgtggtg
     1561 cgcttagtct ggggctgaat cattaccgcg ataacgccat cacttacaaa gcgcagcgcg
     1621 ataaaaaagc cagagagctg gagctggcaa acgcaaccat tactgatatg caggtgcgcc
     1681 agcgtgatgt tgctgcgctc gatgcaaaat actcgaggga attagccgat gcgagagctg
     1741 aaaatgaaat tcttcgcgct gacgttgccg ctggtcgtaa gcgcctgcgt atcaacgcca
     1801 cctgtccagg tcccgtgcgt gaagccaccg gcaccgcccg cgtggataat gcaaccggcc
     1861 cccaactggc agacaccgtt acacgggatt atttcaccct cagagagcgg ctgatgacga
     1921 tgcagaagca actggaaggg gcgcaggaat atatccgtac tcagtgcatt aagtagtctt
     1981 tttattatcc ggaggatata tgaagaaatt actggtaacc gtaaagcctt ttcagggaac
     2041 aattccgttc cgtgttttgc agcctggtcg tgttcttgtt gaaggtacgt tcagtggtaa
     2101 atgtacgcaa tcatactccc gggtctttcg ggtggatgcc tcgaatgaag agctaaccgt
     2161 ggagtgtgct gtgaatgccg ctaaatgccg catggtatcg gctgcattac agccagtgtg
     2221 ttgagcgacc ttattatcca tggcgcggta ttgtcgccgt attcccgtat taacagagac
     2281 cgcagcccga cagggagact cctctgcgcg agtgtgcggg gataatcaaa aacga
//
LOCUS       DAZGAN020000267         2318 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGAN020000267 DAZGAN020000000
VERSION     DAZGAN020000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.269"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..733
                     /locus_tag="ACHKYO_RS25740"
                     /old_locus_tag="pgaptmp_005148"
                     /pseudo
     CDS             1..733
                     /locus_tag="ACHKYO_RS25740"
                     /old_locus_tag="pgaptmp_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289016.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            721..1260
                     /locus_tag="ACHKYO_RS25745"
                     /old_locus_tag="pgaptmp_005149"
     CDS             721..1260
                     /locus_tag="ACHKYO_RS25745"
                     /old_locus_tag="pgaptmp_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm26 family immunity protein"
                     /protein_id="HGU4431791.1"
                     /translation="MAAIKMGVLMNNQLSEICSDSIVSMVKKGVRRFKRPEVGDLFTL
                     EMQDIGFIHGMVARNDLEFVKGQADFNIIYIYKEITKNQSDNLSLAKSELMFPPFIVN
                     DMAWRQGYFQTYDSIPISEIDVFNEYCFYSKAKKQYENERWESCKAFEPCSDLVLSSS
                     LTIAIKIYMRLNPKMKILD"
     gene            1333..2318
                     /locus_tag="ACHKYO_RS25750"
                     /old_locus_tag="pgaptmp_005150"
                     /pseudo
     CDS             1333..2318
                     /locus_tag="ACHKYO_RS25750"
                     /old_locus_tag="pgaptmp_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
BASE COUNT          628 a          505 c          663 g          522 t
ORIGIN      
        1 gccgtccgct gatgctcttt aacagtgaag gtaaaaccgt ctggcgaccg ggcagaccag
       61 cctgtggggg ctggcactca gcctgcccgc agacaccggc tacccggacc cgcgcgggga
      121 actggacccg gaagccgacc ccggcctgct gtatgcgggg cagtggcggg atgcggaatc
      181 ggggctgtgc tataaccggt tccggtatta cgagccggag agcgggatgt acctggtgag
      241 tgatccgctg gggttgcagg ggggtgagca gacttaccgg tatgtgccta atccgctggg
      301 gtatgttgat ccgttagggt tggctgtatg tcctaaattg tatgaaatat acaagggatt
      361 aagagcacaa ggatttaacc cagcagaagc tagagatcta gcaaaatggt tcaatagtag
      421 ctggggaaag gctgatgtat atcttcgttc agcaatagga tcaggaaaaa tagattatgt
      481 tggaataagt agaaatgtgc ttaatcgtta tccgcaaggt aaatatgatt tggagttact
      541 cacaagacta acaggacaac ttccgaggaa tcaagcaaga gcaatagaac aagctatcat
      601 taatgctaaa agaggaaacg gcttcacaaa tatccagaat agtatagatc ctgctaaaga
      661 tcattttgat gcagcggtaa aatggggtga ggagtggtta aagagtaatg gatttggtgg
      721 atggctgcaa taaaaatggg ggtattaatg aataaccaat taagcgaaat atgttcagac
      781 tccattgttt caatggttaa aaaaggagtg cgaagattca agagacctga agtgggcgat
      841 ctttttacac tagaaatgca agatataggt tttattcatg gcatggttgc tcgaaatgac
      901 cttgagtttg taaagggaca agctgatttt aatattattt atatttacaa ggaaataaca
      961 aaaaaccaaa gtgataattt atctttggca aaaagcgaac ttatgtttcc accttttatt
     1021 gttaatgaca tggcatggcg acaaggatat tttcaaactt atgatagtat tcctatatca
     1081 gaaattgatg ttttcaatga gtattgtttt tactcaaaag caaagaaaca atatgaaaat
     1141 gaacgatggg agtcttgtaa agcattcgaa ccatgttctg atttagtttt gtcgagttct
     1201 ttgacaatag cgataaagat ttatatgcgt ttaaatccta aaatgaaaat attggattaa
     1261 tctgtgtcgt tcagactcta tattgagtta ttgcgaaccg ggtgttgcag gtgcaccctt
     1321 ccgtgaccct gcaaccgcca cggcaacctg ctggcctgga cggacggcaa cggtgttgtc
     1381 tggacgatgg aatacggccc gttcgatttg ccggtggcgc gaacggacgg tgaaggccac
     1441 cgctggcagt accgctacga taaagacacg ctgcaactga cagaagtcat taacccgcag
     1501 ggcgaatcat acctttatgt tctggataac tgtggccggg tgacggaaga gcatgactgg
     1561 ggcggcgtgg tctggcgtta ccgctatgac gccgatggcc tgtgtaccgc cagggttaac
     1621 ggcctggagg aaactatcct ctacagccgg gacgccgcag gccgcctggc agaaatcatc
     1681 acgccggaag gcaaaacgca gtatgcgtat gacaaatccg gcaggctgac gggtatcttc
     1741 agcccggacg gtacatcaca gcgcaccggc tatgatgaac gcgggcgggt gaatgtcacc
     1801 actcagggcc gacgggccat tgaataccac taccccgatg aacacaccgt catccgctgt
     1861 atcctgccac cggaagatga acgcgacaga caccccggtg aatccctgct gaaaaccacg
     1921 taccgctaca acgccgccgg agaactgacg gaggttatcc tgccggggga tgagacgctg
     1981 acgttcagcc gtgatgaggc aggacgtgaa gtgctccggc acagtaaccg gggttttgcc
     2041 tgtgaacagg gctggaatgc agccggtcag cctgtcagtc agcgcgccgg ttttttcccg
     2101 gaagaagcca catggggcgg gctggttccg tcactggcgc gggagtaccg ttacgacagc
     2161 gcgggtaatg tgtcaggcgt caccagccgg gaagattacg gacgggaaac ccggcgggaa
     2221 taccggctgg accggaacgg ccaggtcacg gcggtgacag cctcaggcac cgggctgggc
     2281 tatggcgaag gcgacgagac ttatggctat gacagctg
//
LOCUS       DAZGAN020000268         2286 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGAN020000268 DAZGAN020000000
VERSION     DAZGAN020000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.270"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..240
                     /locus_tag="ACHKYO_RS25755"
                     /old_locus_tag="pgaptmp_005151"
     CDS             <1..240
                     /locus_tag="ACHKYO_RS25755"
                     /old_locus_tag="pgaptmp_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431792.1"
                     /translation="VKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQL
                     TIRYSNQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            255..533
                     /locus_tag="ACHKYO_RS25760"
                     /old_locus_tag="pgaptmp_005152"
     CDS             255..533
                     /locus_tag="ACHKYO_RS25760"
                     /old_locus_tag="pgaptmp_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431793.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            539..1156
                     /locus_tag="ACHKYO_RS25765"
                     /old_locus_tag="pgaptmp_005153"
     CDS             539..1156
                     /locus_tag="ACHKYO_RS25765"
                     /old_locus_tag="pgaptmp_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431794.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            1247..1981
                     /locus_tag="ACHKYO_RS25770"
                     /old_locus_tag="pgaptmp_005154"
     CDS             1247..1981
                     /locus_tag="ACHKYO_RS25770"
                     /old_locus_tag="pgaptmp_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4431795.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            2213..>2286
                     /locus_tag="ACHKYO_RS25775"
                     /old_locus_tag="pgaptmp_005155"
     CDS             2213..>2286
                     /locus_tag="ACHKYO_RS25775"
                     /old_locus_tag="pgaptmp_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hok/gef family protein"
                     /protein_id="HGU4431796.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGS"
BASE COUNT          640 a          437 c          569 g          640 t
ORIGIN      
        1 gtaaaagccg gggtaagtat cacttcgcca gttatccgga gtgccgttat tcagaacgga
       61 aactttcagg ttgattctca gggtaacctg aatattggag gccttttcag tgttacgtca
      121 caagggcaac tgacaattcg ttactctaat cagaatgtag gactggtgat ccgcaatgat
      181 aaaattgagg tttatgatca gaatggacga ctggctgttc gcataggcag attacgctga
      241 tcaggaggtg agtattggaa tacggttttg ccatttataa cagaaataac gttaatgtta
      301 cgggcgtgct gactccggta tttttcctgg acagatttac agcggagtct ggctcaaaga
      361 cgtacacgaa taaacccgac gggaaatcat tgcaggctgt atgttgttta tttccctgga
      421 ataacgtatt tgcggatcgg aaagtaccga agataaccat taatggcaat acggtgacgt
      481 ggtcgaatct tgagcagggt atgggatctt atatttatac attctgggga taagtgtcat
      541 gtatggtttg agcattatga agccggatgg cagcgtatgg ataagtccag gttttacgcc
      601 gcagtgtctg atcaacaaag gcaccatacc ggcgactgaa aagtcttttt ttaaaacatc
      661 aatcccgtca ggcaaaagtt gttttttctt tatcagaaca gagaagaagg ccgatgtcat
      721 gtacacgcat gaacagattg atggatatca tgcactaagg cttcatgtaa ttgtcagggg
      781 aacgaaccct ggtgttacga cggtttatgc tttcgcgaat atggttactc caccttctga
      841 gtatggtatc gccatgtata acccggacgg tgagatgatt tatcatggcg aaatgatgct
      901 gcttgacgcg aagttaatac ctgttgatat caaatttgaa aaggaccttg gatatccatg
      961 cgcaatcatg cctgcactgg tcgggtatta taactggaaa agaactcctt atgatcgacc
     1021 gatttatacc acatccactg gtgctacagg aaataaaata tattcctgtg agcattattc
     1081 cggtggtgca acatgggata ttcgaaagcc gtatatagat aaggtcctgg ttattaatac
     1141 atcagtatat gattagttga agcgagtctt taatattcat ttaaaatgtc taaaaagatg
     1201 tattattaaa aagtttagcg tgttatctga atacaggata tcttaaatga agagtatagc
     1261 aacactggtt gtgtgtgcaa tctccgggat tgcctgtgta aatttatctg cacatgcagc
     1321 agaaggaaac catacaattt ctctggggta tgcgcacttt cagtttccgg gactgaagga
     1381 ttttgtaaag gatgcgactg ctcataacag ggagactttc agtcatttcg tcaacagaaa
     1441 ctacttttct tcattgggcg aatatacaga tggtcgggtc agtggatatg aaggcaagga
     1501 taaaaatcca cagggcatta atatcaggta tcgctacgag ataacggatg attttggcgt
     1561 tatcacctct tttacatgga cgcgttctct cactaactca cagacattta ttgatgtgca
     1621 gtcagccgat cataccagga agattaagaa tccggcagct tctgccagaa cggatatcag
     1681 ggcgaattac tggagtctgt tagcggggcc ttcatggcgg gttaatcagt acatgagttt
     1741 atatgcgatg gcagggatgg gcgttgctaa agttagcgct gacctgaaaa ttaaggacaa
     1801 tattaacagt agtggcggat tttctgaaag caacagcacg aaaaaaacct cccttgcgtg
     1861 ggctgcaggt gcacagttta acctgaatga gagtgttaca ctggatgtgg cttacgaagg
     1921 ttccggctct ggcgactggc gcacgagtgg cgttactgct ggcattggcc tgaaattctg
     1981 acctgtatcc ggtaaccgtt tactacccgc tgtgatggcg ggttttttat tgcccgtaca
     2041 gggcaaaaac cgtaaattat gcgtgggtgc ctttcggctg atggctggag ggagaacctg
     2101 aaggcctgat gtggaaaggc cccgagtcaa cttaacgtta acccgaggcc ctaatcatca
     2161 taccttaagc aagtagaagg ttagcgcctc tctgtaaaag gagtcaagcg ctatgtcgca
     2221 aaaatcgctt atcaccgtca caatttgtat gacggttatc ttcactatct ggatgttgca
     2281 cggttc
//
LOCUS       DAZGAN020000269         2225 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGAN020000269 DAZGAN020000000
VERSION     DAZGAN020000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.271"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(110..1108)
                     /locus_tag="ACHKYO_RS25780"
                     /old_locus_tag="pgaptmp_005156"
     CDS             complement(110..1108)
                     /locus_tag="ACHKYO_RS25780"
                     /old_locus_tag="pgaptmp_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HGU4431797.1"
                     /translation="MKNPELKNLTDYSPADKPWDEHRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVTEHPGARWLFLTLTVRNCSIDALGETLTSMNAGFKRLKERKEFRHVLGWVRTTEVT
                     RGKDGSVHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRK
                     PKDDESLPCVVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGAL
                     KNVLQLERETNEDLIMADGMAEGTDDGSRLAFSWVPIVKKYQRDRAKDKRQLSGED"
BASE COUNT          547 a          647 c          540 g          491 t
ORIGIN      
        1 aaaaaaaacg ggcaaaacct tgcccgtttc tgaaaatcac gttagaacgc ttctgagcgc
       61 gtttctgaat attctcccag tgcatgaata gttcgcgcct gaaacgcgct caatcctcgc
      121 cacttagctg acgtttatct ttcgcccgat cgcgctggta ttttttgaca atcggcaccc
      181 acgaaaacgc cagacgggaa ccgtcatctg taccctctgc catgccatcc gccatgatta
      241 aatcctcgtt cgtttcccgc tccagttgca gaacgttctt aagcgctccc cccgtagcaa
      301 caaaacgacg cttgtgtacc tgccgtgtta attcgaggaa ccattcagga tcagccagca
      361 tatcagcagg ctttgtggaa tatttcagag tttcaaccac cgcgccgcgc agctgttccg
      421 caacaacaca aggtaaagat tcatcgtctt ttggtttccg tgacttcacc actctgatat
      481 ccacattcgg gtcatacgcc acccgcagac aatcacgcca cagttccacc caacgggcat
      541 gtttcacata ctcacggccg gacaacatcg aagggggaac catcagcaga gtgtgaaaat
      601 ggggatgtac gctaccatcc ttgccgcgcg ttacttccgt ggtacgaacc catcccaaca
      661 catggcgaaa ttccttacgc tctttcaacc gcttaaaccc cgcattcatc gaggtaagcg
      721 tctcaccaag cgcgtcaatg gaacagttgc gcactgtcag tgtgagaaac aaccagcgcg
      781 cgccgggatg ttccgtcaca atctttggca gggactgata gaaacgggct tgccacatca
      841 tggaacgcct ccactgacag atcggacagt gccggacgcg acagaaccgt gcatcacgca
      901 accgcaggcg cgtttctccg gtctctgtgt ttgttgacca cccaaaccgc aaaataccac
      961 tacaagcccc gacgcgttcg ccgtagtgct caaatccggc agcacgcaga taaataccgc
     1021 ccacatcatc ggaaaagctt cggtgttcat cccacggttt atctgctgga gagtaatccg
     1081 tcagattctt aagttcagga tttttcatgt ctgccgtccc cttgcgagcg acagacgaaa
     1141 ttgcgtgtta tcattcctgt atcacctgta ccaattgtcg tcaaatcagt tcatgttcta
     1201 ggtggtcgcg cccctggagt ggtggaagac tcgcaggggc taatttataa ccactcccga
     1261 caaaatactc tcaaacctat ttacacgcaa atccgcgctc aggccagata tggcaaggga
     1321 tagaggcgat ccaaaaaatc gcatttatcc cttaagtata aatacaagga aacgcgcgcg
     1381 aagatagcag caaaatcgcc tgttttttgt tcgattcccc gagggcaaaa acaacaaccg
     1441 cgaaagcgtt ttcccgacaa aacacacaca ggcttttcca ccgattttgt gaacagcaaa
     1501 aaccgtcttg caggtggttg gtctcactcc gtgacaccaa ccaccatccc cagtgttttt
     1561 cccgtgccgt cgtcaatcac aaaatcggaa ccccggaggg gcccccgcga ttttctgttt
     1621 gacgagttcg tgaacggtga gcgggacgcg gaattttttc gcgtgccgtg aggcgttggc
     1681 ggggtgtcgg ggtgcagccc tgaccaggtg gggaatgtct gagtgcgcgt gcgcggtccg
     1741 acattcccac atcctgtccc gaaaattccc gccgtttctc ggttttttga gcgtcgcaga
     1801 ccgcacgaca aacgaaacga agtgtagttt gtatgagtgc gcacttcgtg agattccccg
     1861 actgccagtt tgtcgatcgc gcggaacagc tgtggcacga aaacggcaga atgatttgct
     1921 gtccggttgc cagaactgcg cgcttacgtg gagggcggcg cggcgcaaaa actgccgaca
     1981 ttgcgccagt gttccggtga cacaaacccg cagaaatcgg gacaggatgt gtaatcgtat
     2041 aaccgcgcac gcacgccgat acgattacct cctggtcagg gctgcacccc gacaccctcg
     2101 acaacctgcc cgggacacgt taaaacgaca tctgcgcgtt tctggtgagc gaaaagctgt
     2161 cagccgtgga ttgccgtagc agagacccgg aaacgcgctt aaaacgcttc tgagcgcaga
     2221 gaaac
//
LOCUS       DAZGAN020000270         2194 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGAN020000270 DAZGAN020000000
VERSION     DAZGAN020000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.272"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(22..192)
                     /gene="ynfU"
                     /locus_tag="ACHKYO_RS25785"
                     /old_locus_tag="pgaptmp_005157"
     CDS             complement(22..192)
                     /gene="ynfU"
                     /locus_tag="ACHKYO_RS25785"
                     /old_locus_tag="pgaptmp_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HGU4431798.1"
                     /translation="MSERKDSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKAVA"
     gene            412..627
                     /gene="essD"
                     /locus_tag="ACHKYO_RS25790"
                     /old_locus_tag="pgaptmp_005158"
     CDS             412..627
                     /gene="essD"
                     /locus_tag="ACHKYO_RS25790"
                     /old_locus_tag="pgaptmp_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HGU4431799.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            632..849
                     /locus_tag="ACHKYO_RS25795"
                     /old_locus_tag="pgaptmp_005159"
                     /pseudo
     CDS             632..849
                     /locus_tag="ACHKYO_RS25795"
                     /old_locus_tag="pgaptmp_005159"
                     /inference="COORDINATES: protein motif:HMM:NF018713.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
     gene            939..1472
                     /gene="rrrQ"
                     /locus_tag="ACHKYO_RS25800"
                     /old_locus_tag="pgaptmp_005160"
     CDS             939..1472
                     /gene="rrrQ"
                     /locus_tag="ACHKYO_RS25800"
                     /old_locus_tag="pgaptmp_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4431800.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1469..1966
                     /gene="rzpQ"
                     /locus_tag="ACHKYO_RS25805"
                     /old_locus_tag="pgaptmp_005161"
     CDS             1469..1966
                     /gene="rzpQ"
                     /locus_tag="ACHKYO_RS25805"
                     /old_locus_tag="pgaptmp_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HGU4431801.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
BASE COUNT          636 a          424 c          530 g          604 t
ORIGIN      
        1 acttgttact aataaaatca atcaggctac agctttaaga tttgtctgga atactttgtt
       61 gcaatgaggg cagatcaaaa gggcaccttt ttgtactctt gaaaaactgt gttctgactc
      121 ttgggtgcag tttgggcagg aacatttaac gagataatta cggcgtgatt ttgagtcttt
      181 acgttctgac ataggctttt cctgtataaa tggccgtata cagtacacta aatatgaaaa
      241 catttctcgt attattattt tatatatgac tttctttcaa aataattacc cacattttta
      301 atgtgtatgt ttttttagcg ccgttgagaa caacgtgtgc tgtcaaaact accccgtaga
      361 ctccgatctt ttcaaacata ttgcaccatc cgtgtacatc ggggtgagga tatgaaatca
      421 atggataagt taacaacagg tgttgcctat ggcacatcgg cgggtaatgc tggtttctgg
      481 gcattgcagt tactcgataa agtaactccg tcacagtggg ctgcaatcgg tgtgctgggt
      541 agcctggttt ttggcctgct gacgtatctg acaaatcttt atttcaagat taaagaagac
      601 aggcgtaagg ctgcgagagg agaataatcc aatgactcaa gactatgaac tggttgtgaa
      661 aggagtccgt aattttgaga ataaagttac ggtaactgta gccttacagg acaaagaacg
      721 ctttgacggt gaaatttttg gcctggatgt cgccatggac cgtgttgaag gagctacgct
      781 ggagttttat gaggcagcag ccagaatgag cgtccggcaa gtcttcctgg aagtagcaga
      841 aaaattgtca gaaaagttga gtcttatctg cagcatcagt actcctttaa gattgaaaat
      901 cctgccaata agcacgagcg tcctcatcat aaatatctat gaacacaaaa atcagatacg
      961 gcctgtcggc tgccgttctg gcgctgattg gtgctggcgc atctgctcct cagatacttg
     1021 accagtttct ggacgaaaaa gaaggtaacc acacaatggc ataccgcgat ggttctggca
     1081 tatggaccat ctgtcggggt gccacagtgg tggatggaaa aaccgttttt cccaatatga
     1141 aactgtcgaa ggaaaaatgc gaccaggtca acgccattga gcgtgataag gcgctggcat
     1201 gggtggagcg caatattaaa gtaccactga ccgaaccaca aaaagcgggt atcgcgtcat
     1261 tttgtcccta taacattggc cccggtaagt gtttcccgtc gacgttttat aagcggctga
     1321 atgctggtga tcgtaaaggt gcatgcgaag cgattcgctg gtggattaag gatggcggac
     1381 gcgattgccg cattcgttca aataactgtt acggtcaggt tattcgtcgt gaccaggaga
     1441 gcgcattaac ctgctggggg atagaacagt gaatcagata ttcatggtga tttttctcgt
     1501 gttgtcagga tttatcgtcg gaaatgtctg gagcgaccga ggatggcaaa aaaaatgggc
     1561 ggaacgtgat gctgccgcat tatcacaaga ggtaaatgct caatttgctg ctcgaataat
     1621 tgaacagggg cgaactatag cccgtgatga ggctgttaaa gatgcgcaac agaaatctgc
     1681 tgaaatttct gccagggctg cttatctgtc tgatagtgtt aaccagttgc gtgccgaagc
     1741 aaaaaaatat gccatacgcc ttgacgcagc gaagcatacc gcagatcttg ccgctgccgt
     1801 cagaggcaaa acaaccaaaa ccgccgaagg aatgctcacc aacatgctcg gagatattgc
     1861 agcagaagct cagctttatg ctgaaattgc tgacgaacgc tacatcgcag gagtgacttg
     1921 tcaacagatc tatgaatctt taagagataa aaagcatcaa atgtagggta atattaaatc
     1981 ggaacattta catcgcggaa tgtaaaattt aaataaaaag gactcttcca tgagccaaaa
     2041 ttcctgaaat cttaagggta agataaaagg tcttaatcag aatgacacgt tttattaata
     2101 aataaagcta ttctttcatt gctgtgtttt tctttacaaa agtaatcctt gctatgggtg
     2161 gttaatcatg cgttaatggt gttctggttt gtta
//
LOCUS       DAZGAN020000271         2184 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGAN020000271 DAZGAN020000000
VERSION     DAZGAN020000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.273"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            547..755
                     /locus_tag="ACHKYO_RS25810"
                     /old_locus_tag="pgaptmp_005162"
                     /pseudo
     CDS             547..755
                     /locus_tag="ACHKYO_RS25810"
                     /old_locus_tag="pgaptmp_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            742..931
                     /gene="tnpB"
                     /locus_tag="ACHKYO_RS25815"
                     /old_locus_tag="pgaptmp_005163"
                     /pseudo
     CDS             742..931
                     /gene="tnpB"
                     /locus_tag="ACHKYO_RS25815"
                     /old_locus_tag="pgaptmp_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(934..1122)
                     /locus_tag="ACHKYO_RS25820"
                     /old_locus_tag="pgaptmp_005164"
     CDS             complement(934..1122)
                     /locus_tag="ACHKYO_RS25820"
                     /old_locus_tag="pgaptmp_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HGU4431802.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
     gene            1657..1798
                     /locus_tag="ACHKYO_RS25825"
                     /old_locus_tag="pgaptmp_005165"
                     /pseudo
     CDS             1657..1798
                     /locus_tag="ACHKYO_RS25825"
                     /old_locus_tag="pgaptmp_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          583 a          515 c          452 g          634 t
ORIGIN      
        1 atattttcta gtgtatttga cttttctttc tcaagtgtta tttcttgacc tttatcacag
       61 aaccatctcc ctggatatcc ccatcacatt gcatgaaaag caagttttac caatcttgat
      121 tttcagtgtt aaataagatt aataactgta attctgcaaa aaaaagaaca ccatatgtag
      181 tatgactacg tattccactc tgttcgttca cggactggca tcgaagtctg aggtgtactg
      241 gcaatagcgg atacagtcat ttgttctttt tttaagcatt catccgatga tacttttcct
      301 gaatgctacc ggagtggtat tcccctgccg tgagggaggg cgctgacggt tataaaaatc
      361 ctgatatata cccattcccg tatgacagcg atggcgtcac atctctgttc taccatcttc
      421 acagaaatat ttcgttcatt agcattccgg tattcgtata accaccttcg gtggagaaag
      481 aataaacaat ccctggaatg cgatgagcgc ttgaccagaa aagcatcata ttgcttcatg
      541 gcgcatcagc gaccacagga tgccgcaaaa gccgaaaaac ggaccagagc gcctgcaatc
      601 attcctcgcc tcagtatctg ttgcccatgg agtggccgat aacagatcat tgtcggatga
      661 gccttatccc cttccttccc ggtggtatcc ggatgtgctg ccagccttct cagttaaccg
      721 atgttttccg ggctttttgc catactgagg actgataacg tattcctggt cgtaaaaccc
      781 gtcaatatgc gccggggcat cgacaaaact gacacagtac gtccggaaag aacttaacgc
      841 tgcatggcac gacaatgctg cctttgtctt taccaacaag gctcgttccc gcataaaagt
      901 tctacgccgg gataaacatg gcgtatggct gcgtcaggcc ttcactactt cacaccggaa
      961 gtcttccacc ctgacacgaa tataccgtcc gggctctttc gcaggtgcaa acagtttttc
     1021 acattcggcg aatgtcattt ctgaatcctg gtaacgcaac cactgaaccg gcatattacc
     1081 gggtttgatt attgtcgcag ttattttata tcgtttcccc atatcgcctc tcatcctgac
     1141 aacaaaatta ccggctgagc catcactccg gtaaagcatg cgaaaaaaag tgagcgtctc
     1201 agcaatggct catgcgatga ttctgtacgg gcaccgatgg cacctcagaa tacaacaaag
     1261 atatcagagt aacatgtctc tctcgttatt tttcagagtt cttgacaccc tccttctgcc
     1321 acattgtcaa cgccagtgga gacttctgct gtcagttttt tcatccatac agagtgacat
     1381 taattatcct gaacaaaacg aaatatcctg aatatattta ttttctcctg gtgccggaga
     1441 catgatgatg tacaggctca ggtgcggtgt gaatgtaaaa tggtatgtta attattcatt
     1501 tctgaatgca aactgaatgc aaacttattc cgaattgaat tctcctgaca ccacatgata
     1561 aaaatgagga tataataaaa tacttctctt ctggatgtgc cattacctgc cgttgctgta
     1621 atacggcggg tattttttac tgacatatcg ggaagccagc agacgttcgt agctacatcc
     1681 ccgaaagtgg cacactgcaa actataatac gagtaaaaca tcgatcaccg tagctgatgt
     1741 gttcaacgat cgtgcactag cgttctgcga ttctcaggga ctgcctgttc tgagaatcgg
     1801 cgagtccgcg atcggatacg atgcgatttc gcagtgactc agggattgca tctgaaaacc
     1861 ctccggctat gtcggtaaat atttatttct atgaaccgta cgattcagat agcattcgcc
     1921 cggccccgta agaataatct ccggacaaat aagatttttc ctgaaaaagc gtctagtctt
     1981 ttgatatcgt tgtttttatg ctaatcaggg agtatgtgtg atgattgtct gttacgttat
     2041 tcttgttaac cgtccgtttg aaacagagat tgccgggaaa aagatatttt ttcagaaaaa
     2101 tgacgtcctg ttgacagata acatcagcca gtcctttttc ctgcgccatc ccggtaatgt
     2161 tactgagctc cggattgatg aatg
//
LOCUS       DAZGAN020000272         2128 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGAN020000272 DAZGAN020000000
VERSION     DAZGAN020000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.274"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1496
                     /locus_tag="ACHKYO_RS25830"
                     /old_locus_tag="pgaptmp_005166"
     CDS             <1..1496
                     /locus_tag="ACHKYO_RS25830"
                     /old_locus_tag="pgaptmp_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HGU4431803.1"
                     /translation="AKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPEAGGNSDVQ
                     GGYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGS
                     EKAKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVDV
                     EQFHYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEE
                     PQRQNQAKKAAYDVFYSFSMNRDRIQSDVLNKAAELISDMGNKIGDYLGESYKSLARE
                     IADDIKNFQGKTIRSYDDAMESLNKILSNPSFKVNRADRDALVNAWRSRDAQDIANKL
                     GNISKAFKAADVIMKIEKVKEKSIEGYETGNWGPLMLEVESWVLSGMAASVAMTLLSA
                     IIGSFTLPALALTAINIAGIMGISYLASYIDASVAEKINNKLVHFTH"
     gene            complement(1519..2051)
                     /gene="cui"
                     /locus_tag="ACHKYO_RS25835"
                     /old_locus_tag="pgaptmp_005167"
                     /pseudo
     CDS             complement(1519..2051)
                     /gene="cui"
                     /locus_tag="ACHKYO_RS25835"
                     /old_locus_tag="pgaptmp_005167"
                     /inference="COORDINATES: protein motif:HMM:NF015795.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein Cui"
BASE COUNT          696 a          281 c          512 g          639 t
ORIGIN      
        1 tggctaaaga actaagtgga tatggcccaa ctgctggtga gtctatgggg ggaacagggg
       61 cgaatctgaa tcagcaaggt ggaaataata atagtaatag tggggttcac tggggtggtg
      121 gttcaggctc tggtaatgga ggtggtgaac atggaagtca gacggggtgg ggatggagta
      181 aaaccaataa tccggatgtg ccgccttatg ttgatgataa tgggcaggtg cgcattacca
      241 ttacaaatgg tctggtaaaa acgcctgtat atggagttcc tgaggctggc ggaaatagtg
      301 atgttcaggg gggctatatt cctgaaaatc ctaatgatga agttgctcgt aagtgggata
      361 aaaacaactt accgcgagag attgatgtga gtatcgatgg ttttaaatat cgtgtaacgc
      421 ttaatgataa tggtagagct atcggtattc ttcgaacagg tgtccgtcct tacgtaggga
      481 gtgaaaaagc taaggcgggt attatggaaa aaatcaatca taaaacacct gaggagatat
      541 atgaggcgtt aggttttaat aaagatgaat ctcagcggca ggaaaaagca aaacaacagg
      601 ctgaagatgc gtgggatcgt ttgcctccta atgtgagaaa atttgatgtt gatgttgaac
      661 agttccacta tttagtggtg cttgatgatt atggtaatgt tttatctgta actagaacag
      721 ctgttcgtcc ttatgtaggg agtgaaaaag ctaaggctgg tattatggat aaagtcgacc
      781 ataagacccc ggaagagata tacgaggcat taggatttaa taatgaagag cctcaacgac
      841 agaatcaagc taaaaaagct gcttatgatg tattttattc tttcagtatg aaccgtgacc
      901 ggattcagag tgatgtgcta aataaagcag ctgaacttat atctgatatg ggtaacaaga
      961 tcggtgatta tcttggtgaa tcttataagt cgttagcaag agaaattgct gatgatatca
     1021 aaaatttcca ggggaaaacc atccgtagct acgatgatgc aatggagtca ttaaataaaa
     1081 ttttgtctaa tcctagtttt aaagttaaca gagcagaccg tgatgcactg gtgaacgcct
     1141 ggcgaagtcg tgatgctcag gatatagcaa ataaactggg gaatatcagt aaggctttca
     1201 aagctgccga tgtaattatg aagattgaga aggtcaagga gaaaagcatc gagggatatg
     1261 aaactggaaa ctgggggccg ctgatgttgg aggttgagtc ttgggttctc agtggtatgg
     1321 ccgcaagcgt tgccatgaca ttgcttagtg ctataattgg atcattcaca ctaccggcgt
     1381 tagcattaac tgcaataaat attgcaggta ttatgggaat tagttacctt gcatcatata
     1441 ttgatgcaag tgtggctgaa aaaataaata ataaattggt tcattttact cattgataga
     1501 aaaagagagg tgatcatatc acttctcttt aaacgaccta taggaaccaa tacaaaacca
     1561 aaaatacaaa tatgagaata taaaaataat ggaatgtaag ctaatgtaaa aaaacgatag
     1621 gaggattata tttacagaca tcagttttag catttttgca gaactggtta gctccgtatc
     1681 agcaaataaa aaagcatata ttaagatagc ataaaaaaaa gtgaaaaaat atttattttc
     1741 agtagttgcc atggagattt atcatttttt aatctaagat ttttacatgt gtttaaaaat
     1801 aaaagaatgg cagtgaaagg agctgttttt acatacagac tcaatgtttt gctaaatact
     1861 ggggtgtttt gtgagtatat ggcaggtagg tcatttgctc tcaatgctat atagtttaat
     1921 atattggatg ttggattgtg gatatatata atgattattg agaacattgg gattagtgaa
     1981 attataagga agtaaaacat ctttgttgct attttatttc ctttgtctgt ttggtggtgt
     2041 ttatttatca tctgtttacc tgtaaattta ttatattaaa gattttgatg aatggctatg
     2101 gtttattttc tctttagaga gtaatata
//
LOCUS       DAZGAN020000273         2117 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGAN020000273 DAZGAN020000000
VERSION     DAZGAN020000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.275"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..636
                     /locus_tag="ACHKYO_RS25840"
                     /old_locus_tag="pgaptmp_005168"
     CDS             <1..636
                     /locus_tag="ACHKYO_RS25840"
                     /old_locus_tag="pgaptmp_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HGU4431804.1"
                     /translation="EPDVAGAMYLKSEDGQDWYECQALFSPETLKVVYDSRGVITGYG
                     KETALLWPVNQSVAEVPDTPESRKIDLSGRWGFDGEKITDLLTAEKARGMKGDEINAW
                     RNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFWTDAENNDVEVTA
                     EELIALSEAAEQAMFTKGMEIHVRQRTMKKVLEKLTSADEILAYRVGRAQE"
     gene            1174..>2117
                     /locus_tag="ACHKYO_RS25845"
                     /old_locus_tag="pgaptmp_005169"
     CDS             1174..>2117
                     /locus_tag="ACHKYO_RS25845"
                     /old_locus_tag="pgaptmp_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431805.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDSHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSG"
BASE COUNT          540 a          458 c          630 g          489 t
ORIGIN      
        1 gagccggatg ttgcaggcgc gatgtacctg aagtctgagg atggtcagga ctggtatgag
       61 tgtcaggcgt tgttttcgcc ggagacgctg aaggtggtgt atgacagccg gggtgtgatt
      121 acgggatacg gtaaggagac tgccttgttg tggccggtga accagagtgt ggcagaagtg
      181 ccggataccc cggagagccg gaaaattgac ctgagcgggc gctggggctt tgacggggag
      241 aaaatcacgg acctgctgac cgcggagaaa gcgcgcggga tgaagggcga tgaaattaac
      301 gcctggcgta atgcgatgga agcggcgaac tacacgtttg agcacaatgg gcgtaaatgg
      361 gactacggga agtcaacgca gacgcgtctt gagccgtcgg tggctgcagc gaaagcgggg
      421 aaactgccgg aggcgttttt ctggacggat gcggaaaaca atgatgtgga agtgacagca
      481 gaagagctta tagcgctgag tgaagcggca gagcaggcga tgttcaccaa agggatggaa
      541 atccacgtcc ggcagcgcac catgaagaaa gtgctggaaa agctgaccag tgcggatgag
      601 atactggcat acagggttgg cagggcacag gaataacaga caaaaagatg ggggaccatc
      661 acctccccca tgaactgagt caattataca cttgtgaagt atttactatg tgaataaata
      721 ctgtcacgga aagtgcaggc ctggatgtga tggaaatcat gtttccggcg catcctgttg
      781 atatggataa tttttcagat ttccggaagt gagagttttt ggtgtttcca taccagccac
      841 agttgatgtg tgttgtggcg cttggcagtg cgattaaagg agcagaggga ataaaaaacg
      901 ggggcatgac caacccccgg tacaaggaag gagtaactac aatggaaaaa ttaatgaaga
      961 cctttagtgc tacgttatat ggcgaataaa gactgccaca gggaagtttg cagaaacagt
     1021 gatgccgatc gtgtttctga cgctcttctc tgataagagt aatttttcag atttctggaa
     1081 atctgtattc tgcacaggcg caattgtatg ccgcctttaa aacttcaatt cagtgactca
     1141 cctgccattc aaattttcgg ataccagaca accatgcctt atatcgatat aacaactatg
     1201 cgcgggatga tgccaggcgt tattgcatct atgctgccag atcattctgc tgtactggca
     1261 gaaaactgtc attttcgcta tggagtgatc acgcctgaac accagatgtc agaggctgag
     1321 aaaacattcg cgattaagcc gaaaaccatt tttcattacc gtgacgattt ctggtttgca
     1381 tggacggatg tggtggatgt gatccgcagt ccggtcgctc aggactccca cgggcgtatt
     1441 tactacactg acgggcgttt tcctaaagtg acggatgcga ccattgccac aaaaggggac
     1501 gggaatcacc cgacatcatc gtatcgtctg gggatccccg cgccgacgac agcacctgtc
     1561 tgtactgttc agcagggcgg tgatgtttct gacgataacc cgaatgatga cgaaacccgg
     1621 ttttatacgg aaacctttgt ctcagattat ggtgaagaag gtccgccagg tccggcgtct
     1681 ctggaggtaa cactccgtac tccggggact gcggtacagc tgacgctgtc tccggtgcca
     1741 ttgcagaatg ccagtattaa acgccgccgg atttatcgct ctgcatcagg tggaggagaa
     1801 gcggattttt tacttgtggc tgaactggat gcatccgtgc tcagttacac ggacaaaata
     1861 ccggggaaaa accttggacc ttctctggcg acatgggatt acctgccgcc gccagagaat
     1921 atgacaggcc tttgcctgat ggctaatggt attgccgccg ggtttgccgg taatgaagtg
     1981 atgttttcgg aagcgtatct gccgtatgca tggccggaag tgaatcgtca cacgacggca
     2041 gaagatattg tggctatctg tccgctggga acgtcactgg tggtggcgac aaagggggag
     2101 ccctatctgt tcagtgg
//
LOCUS       DAZGAN020000274         2047 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGAN020000274 DAZGAN020000000
VERSION     DAZGAN020000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.276"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..423
                     /locus_tag="ACHKYO_RS25850"
                     /old_locus_tag="pgaptmp_005170"
     CDS             <1..423
                     /locus_tag="ACHKYO_RS25850"
                     /old_locus_tag="pgaptmp_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4431806.1"
                     /translation="AFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDET
                     NPEMPPPVVISPGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKK
                     GDAGKKSAKGDAAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            420..>2047
                     /locus_tag="ACHKYO_RS25855"
                     /old_locus_tag="pgaptmp_005171"
     CDS             420..>2047
                     /locus_tag="ACHKYO_RS25855"
                     /old_locus_tag="pgaptmp_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4431807.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQNGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWTGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQIL
                     DSLVEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEP"
BASE COUNT          448 a          499 c          682 g          418 t
ORIGIN      
        1 gcgtttatag cccgtaaaca gtgcgagacg gtggaggtgg atgaggttcc tgtttaccgg
       61 cagaaaaaat cagagcagga ggataaaccc cgtcgccgtg aggcggctgc aataccacag
      121 ccggacgaaa caaatccgga gatgccaccg cccgtggtga tatctcctgg tattgagtat
      181 atggaggacg gtcttcccga tccggtgaaa gcgatggggc gtcttctggt ggagaacatt
      241 aataccgacc ccaggctggc gctggatgcg gcttataagc tggcgcagtt cacgcaccac
      301 aaaaaagggg atgccggtaa aaaatcggca aaaggtgacg cggcgaaaaa agcggctaac
      361 cgttttgcgg tgccaccacc accccgactg gtggtgaata atgataatga gggcaacgga
      421 tgatacctgt gtggagcacg gcctgcccgg actgggcaga gcgcctgaaa aaggggctgt
      481 cgattattcc ggctccgatt tatccggagc aggccgcaca tgccctggcg atttttaaac
      541 aactgcggat tgtggatgca ccgggcagcc cgacgttcgg ggagtcctgt gcaccgtggg
      601 tgtttgacct ggtggcggcc ctgtttggct cctacgatgc gcagaacggt gtacgccata
      661 tcaaggaagt ttttatcctt atcccgaaaa aaaacagcaa gtccacgctg gctgccggga
      721 tcatgatgac ggcgctgtta ctgaactggc ggcaggcggc gggctacacc attctggccc
      781 cgaccgtgga ggtggcggct aacgccttta atcctgcgcg ggatatggtc agacgtgacg
      841 atgatctgga tgacctctgc caggtacaga cacatatccg gaccatcacc cacagggtga
      901 cggacaccac ccttaaggtg gtggcagccg atccgaatac ggtgtccggt atcaagtctg
      961 tggggacact gattgatgaa ctgtggctgt ttggcaagca gtacaaagcg gaggacatgt
     1021 tacgtgaagc catcggcggg cttgcctccc gcccggaagg gtttgtggtg tatacgacca
     1081 cccagtcgaa tgagccgcca gccggggtgt tcagacagaa actgcagtac gcccgggatg
     1141 tccgtgacgg caaaattcat gatccgcact ttctgccggt gatatttgag catccgccgg
     1201 agatggtgga gcgcggtgag cacctgctga tggaaaacct cgccatggtc aatccgaatc
     1261 tcggttattc ggtggatgag gcttttctgt accgggagta ccgtaaagcc cgggaggctg
     1321 gtgaggaagc atttcgtggc ttcatgtcaa aacatgccaa tgtggaaatt ggtcttgccc
     1381 tgcgttctga ccgctggacg ggtgcggatt tctgggagca gcagggcagg cgcgtcagcc
     1441 tggacgatat cctgcagcgc gctgatgtgg tgacggtggg gattgacggc gggggcctgg
     1501 atgatctgct gggaatgtac gtgattggcc gtgacaggga aacccgcgaa tggctgggct
     1561 ggggccatgc ctgggcgcat gaaaccgcgg tggtcagacg gaagagtgag gcatcccggt
     1621 ttcaggattt tgtggcctgt ggagacatga cgattgtccg tcgggtcggg gatgacacgg
     1681 cggaagtggc ggagtatgtg cgtcgtattc atgaggctga gttactggag catatcggta
     1741 ttgacccgtc aggtgtgggg cagattctgg attcactggt ggaagccggg atccccgacg
     1801 gaattgttgt ggggataagc cagggctgga agctgggcgg ggccataaaa accaccgagc
     1861 gcaaactggc tgagggagta ctggtgcatg gtggtcagcc actgatggcc tggtgcgttg
     1921 gcaatgcccg ggtggagccg aaaggtaacg ccatccttat taccaaacag gccagcggac
     1981 gggggaaaat tgacccgctg atggcgctct tcaatgcggt atccctgatg tccctgaatc
     2041 cggagcc
//
LOCUS       DAZGAN020000275         2047 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGAN020000275 DAZGAN020000000
VERSION     DAZGAN020000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.277"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..423
                     /locus_tag="ACHKYO_RS25860"
                     /old_locus_tag="pgaptmp_005172"
     CDS             <1..423
                     /locus_tag="ACHKYO_RS25860"
                     /old_locus_tag="pgaptmp_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4431808.1"
                     /translation="AFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDET
                     NPEMPLPVVISPGIEYMDDGLPDPVKAMGRLLVENINTDPRLALDVAYKLAQFTHHKK
                     GDAGKKSAKGDAAKKAANRFAVPPPPRLVVNNDNEGSG"
     gene            420..>2047
                     /locus_tag="ACHKYO_RS25865"
                     /old_locus_tag="pgaptmp_005173"
     CDS             420..>2047
                     /locus_tag="ACHKYO_RS25865"
                     /old_locus_tag="pgaptmp_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4431809.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEDTFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLISLNPEP"
BASE COUNT          448 a          501 c          679 g          419 t
ORIGIN      
        1 gcgtttatag cccgtaagca gtgcgagacg gtagaggtgg atgaggttcc tgtttaccgg
       61 cagaaaaaat cagagcagga ggataaaccc cgtcgccgtg aggcggctgc aataccacag
      121 ccggacgaaa caaatccgga gatgccactg cccgtggtga tatctcctgg tattgagtat
      181 atggatgacg gtcttcccga tccggtgaaa gcgatggggc gtcttctggt ggagaacatt
      241 aataccgacc ccaggctggc gctggatgtg gcttataagc tggcacagtt cacgcaccac
      301 aaaaaagggg atgccggtaa aaaatcggca aaaggtgacg cggcgaaaaa agcggctaac
      361 cgttttgcgg tgccaccacc cccccgactg gtggtgaata atgataatga gggcagcgga
      421 tgatacctgt gtggagcacg gcctgcccgg actgggcaga gcgcctgaaa aaggggctgt
      481 cgattattcc ggctccgatt tacccggacc aggccgcaca tgccctggcg atttttaaac
      541 aactgcggat tgtggatgca ccgggcagcc cgacgttcgg ggagtcctgt gcaccgtggg
      601 tgtttgatct ggtggctgcc ctgtttggct cctacgatgc gcagaccggt gtacgccata
      661 taaaggaagt gttcattctg atacccaaaa aaaacagcaa gtccacgctg gctgccggga
      721 tcatgatgac ggcgctgtta ctgaactggc ggcaggcggc gggctacacc attctggccc
      781 cgaccgtgga ggtggcggct aatgccttca atcctgccag ggatatggta cgacgggacg
      841 atgatctgga tgacctctgt caggtgcaga cacatatccg gaccatcacc catcgggtga
      901 cggacaccac cctgaaggtg gtggcagccg atccgaatac ggtgtccggt atcaagtccg
      961 tggggacact gattgatgaa ctgtggctgt ttggcaagca gtacaaagcg gaggacatgt
     1021 tacgtgaagc cataggtggc cttgcctccc gcccggaagg gtttgtggtg tatacgacca
     1081 cccagtcgaa tgaaccgccc gccggggtgt tcagacagaa actgcagtac gcccgggatg
     1141 tgcgcgacgg caaaattcat gatccgcact ttctgccggt gatatttgag catccgccgg
     1201 agatggtgga gcgcggtgag cacctgctga tggaaaacct cgccatggtc aatccgaatc
     1261 tcggttattc ggtggatgag gcctttctgt accgggagta tcgtaaagcc cgggaagccg
     1321 gtgaagatac attccggggg ttcatgtcaa aacacgccaa tgtggaaatt ggtcttgccc
     1381 tgcgttctga ccgctgggcg ggtgcggatt tctgggagca gcagggcagg cgcgtcagcc
     1441 tggacgatat tctgcagcgc gctgatgtgg tgacggtggg gattgacggc gggggcctgg
     1501 atgatctgct gggaatgtac gtgattggcc gtgacaggga aacccgcgaa tggctgggct
     1561 ggggccatgc ctgggcgcat gaaaccgcgg tggtcagacg gaagagtgag gcatcccgtt
     1621 ttcaggattt tgtggcctgt ggagacatga cgattgtccg tcgggtcggg gatgacacgg
     1681 cggaagtggc ggagtatgtg cgtcgtattc atgaggctga gttactggat catatcggta
     1741 ttgacccgtc aggtgtgggg cagattctgg attcactggc ggaagccggg atccccgacg
     1801 gaattgttgt ggggataagc cagggctgga agctgggcgg ggccatcaaa accaccgagc
     1861 gcaaactggc tgagggagtg ctggtgcatg gtggtcagcc actgatggcc tggtgcgttg
     1921 gcaatgcccg ggtggagcct aaaggtaacg ccatccttat taccaaacag gccagcggac
     1981 gggggaaaat tgacccgctg atggcgctgt tcaatgcggt atccctgata tccctgaatc
     2041 cggagcc
//
LOCUS       DAZGAN020000276         2047 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGAN020000276 DAZGAN020000000
VERSION     DAZGAN020000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.278"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..509
                     /locus_tag="ACHKYO_RS25870"
                     /old_locus_tag="pgaptmp_005174"
     CDS             <1..509
                     /locus_tag="ACHKYO_RS25870"
                     /old_locus_tag="pgaptmp_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4431810.1"
                     /translation="SAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRDGAGIWTICR
                     GATRVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQKAGIASFCPY
                     NIGPGKCFPSTFYKRINAGDRRGACEALRWWIKDGGRDCRIRSNNCYGQVSRRDQESA
                     LACWGIDR"
     gene            666..848
                     /locus_tag="ACHKYO_RS25875"
                     /old_locus_tag="pgaptmp_005175"
     CDS             666..848
                     /locus_tag="ACHKYO_RS25875"
                     /old_locus_tag="pgaptmp_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431811.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            863..994
                     /locus_tag="ACHKYO_RS25880"
                     /old_locus_tag="pgaptmp_005176"
     CDS             863..994
                     /locus_tag="ACHKYO_RS25880"
                     /old_locus_tag="pgaptmp_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431812.1"
                     /translation="MSDKLIMLAKILCVIIGISFSLMLVALFLSMGWMMLSSSGMLG"
     gene            1217..1423
                     /locus_tag="ACHKYO_RS25885"
                     /old_locus_tag="pgaptmp_005177"
     CDS             1217..1423
                     /locus_tag="ACHKYO_RS25885"
                     /old_locus_tag="pgaptmp_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4431813.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            1488..1712
                     /locus_tag="ACHKYO_RS25890"
                     /old_locus_tag="pgaptmp_005178"
     CDS             1488..1712
                     /locus_tag="ACHKYO_RS25890"
                     /old_locus_tag="pgaptmp_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431814.1"
                     /translation="MQKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVEYTMNAAKCRMVSAALQPVC"
BASE COUNT          497 a          454 c          583 g          513 t
ORIGIN      
        1 tgtcggctgc cgttctggcg ttgattgccg caggggcttc tgcgcctgaa atcctcgacc
       61 agtttcttga cgaaaaagaa ggtaaccaca ctacggcata ccgtgatggt gcgggtatct
      121 ggaccatctg ccgtggagcc acccgggtgg atggtaagcc tgttattcct ggcatgaagc
      181 tgtcgaaggg gaaatgcgac cgggttaacg ccattgagcg tgataaggcg ctggcatggg
      241 tggagaaaaa catcaaagtg ccactgactg aaccccagaa agcgggtatt gcgtcattct
      301 gtccttacaa cattggcccg ggtaagtgtt tcccgtcgac gttttataaa cgaattaatg
      361 caggagatcg caggggagcg tgtgaggcgc ttcgctggtg gattaaggac ggtggcagag
      421 actgccgtat ccgctcaaat aactgttatg gtcaggtatc ccgtcgtgac caggagagcg
      481 cgctggcgtg ctggggtatc gacagataag cagaatattt tgctgaaaaa tgacgttggc
      541 caacgcggac ggataacacg aaatcctgcg aactggcaaa acctaagtga ataaaagtaa
      601 aaaccccgtt tgttggcagc aagcggggtt ttgtgtttct gaccttggat aaggcaaggg
      661 agaacatgga aaagtataaa cgaattctgt tgaggttgac tatgaaaaac ggccttgaac
      721 tgaaagcgcc agtaactgat gacatcagca gagcactggc ttttgctatt aagtgggtgg
      781 cggtcggtat cgctgtgtct ccgatgctgt atgggctggc aaaactggtc attgcgttga
      841 aatcgtgaag ggaggattaa acatgtcaga caaactcata atgctggcga agatcctctg
      901 tgtaatcatc ggcatttcat tttcactaat gctggttgct ctttttcttt ccatgggctg
      961 gatgatgtta tcttcgtcgg ggatgctggg gtgagcataa accgaatgct ttccgcgttt
     1021 accgttattc tgctggtggt ctgtggtgcg cttagtctgg ggctgaatca ttaccgcgat
     1081 aacgccatca cctacaaagc gcagcgtgat aaaaaagcca gagagctgga gctggcaaac
     1141 gcaaccatta ctgatatgca ggtgcgccag cgcgatgttg ctgcgctcga tgcaaaatac
     1201 tcgagggaat tagccgatgc gagagctgaa aatgaaactc tgcgtgctga tgttgccgct
     1261 ggtcgtaagc gcctgcggat caacgccacc tgccccggta ccgtgcgtga agccaccggc
     1321 acctccggcg tgggcaatga tgccgccgtc gaactctctc cggttgctgg acgaaacgtt
     1381 ctcggtatca gagacggaat catcagcgac caggcagcat tgagaatgct tcaggaatat
     1441 atccgcactc agtgtattaa ctagtatttt tgttattcgg agaatgcatg cagaaattat
     1501 tggtaaccgt aaagcctttt cagggaacaa ttccgttccg tattttgcag cgtggtcgtg
     1561 ttcttgttga aggttcgttc agtggtaaat gtacgcaatt acactcccgg acctttcagg
     1621 tgaatgccac gaatgaagag ctaaccgttg agtatacgat gaatgccgct aaatgccgca
     1681 tggtatccgc tgcattacag ccagtgtgtt gagcgacctt attattcatg cgcggtattg
     1741 tcgccgtatt cctgcattaa cagagacagc agcccgaccg ggagactcct ctgcgcgagt
     1801 gtgcggggat aatcaaaaac gatacacacc ggggtttacc gcgttaacgg agcgcggcgt
     1861 tgtcccctca tggtcgctgg tccggtgcga tggtggaaga aaccggacga tgtgttacct
     1921 cgcaagacct gttatgtcat gtgtctgatt tgtgatttaa gtcggataat tgtcgttgcc
     1981 attaagcaga ggattgatgg ccgacagggt ggcattgtta gaataagact tattcttatc
     2041 tgtgccg
//
LOCUS       DAZGAN020000277         1982 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGAN020000277 DAZGAN020000000
VERSION     DAZGAN020000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.279"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..349)
                     /locus_tag="ACHKYO_RS25895"
                     /old_locus_tag="pgaptmp_005179"
     CDS             complement(<1..349)
                     /locus_tag="ACHKYO_RS25895"
                     /old_locus_tag="pgaptmp_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431815.1"
                     /translation="MIIKSFKLYTPEKSDVPGAMYLKSVDGRDWYESQSLFAADTLKL
                     VYDSNGIITSISKDVSMLWPVNQSVAEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRN"
     gene            complement(621..>1982)
                     /locus_tag="ACHKYO_RS25900"
                     /old_locus_tag="pgaptmp_005180"
     CDS             complement(621..>1982)
                     /locus_tag="ACHKYO_RS25900"
                     /old_locus_tag="pgaptmp_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431816.1"
                     /translation="KSASDSAKTATSRAAEAGQKASEAAEAATRAGESEKAAAADAKN
                     AKQHAETARVAQQAAGDILERAEAATVSAEEARRMAENARGPQGDQGPKGDTGPRGEK
                     GEPGQRGPQGPKGERGERGPQGSVGPAGPRGEKGEQGERGPQGLQGLKGETGERGAQG
                     AQGPAGPTGATGPAGPRGETGPQGAQGPAGATGPVGPQGERGIQGPAGPAGPRGETGA
                     RGEKGDPGDPGGPPGPKGDTGPAGPAGEKGEPGERGPQGLQGPPGPAGERGPAGEPGA
                     KGETGKPGPAGPQGQPGPQGLKGDRGEPGPTGPQGPAGERGPQGPRGVAGPVGPAGAA
                     GAKGDAGPAGPAGPRGPAGERGPAGPKGERGEPGPMGPPGPAGDAGNSTVKGIRLGNE
                     MVYRPYSGYSNLSLRSMDGCVLTGIAGTNMPDKSRIDEIYYRPLQMTFGDGSWRSVGI
                     GEV"
BASE COUNT          313 a          691 c          470 g          508 t
ORIGIN      
        1 cattacgcca ggcgttaatt tcatcgccct tcatcccgcg cgctttctcc gcggtcagca
       61 ggtccgtgat tttctccccg tcaaaacccc agcgccccga gatgtcagct ttgcggtttt
      121 cttccgtatc agcaacctct gccacactct ggtttaccgg ccacagcata gagacatcct
      181 tacttatgct ggtgattata ccgttactgt cataaaccag cttcagcgtg tctgccgcga
      241 ataatgactg gctttcgtac cagtccctac cgtctactga tttcaggtac atcgcacccg
      301 gcacgtcaga tttttcaggg gtgtatagct taaaactctt aataatcatc aacgtccact
      361 catttaaata aaactgtgat ttcagtgcct catctttttt cttttcacaa aaaaagcgtt
      421 gtgagatcaa agaaaccaga catgtgcatg cactaatatt ccctgcgctg acttatgtca
      481 tgataataat aactgacagt gtggtatcat tgtctggtct gtatgtttac cggggagtaa
      541 cgccctgctc cccggttttt tatgcgcaat ataacaagta tgcagagctg ccagttacct
      601 gcgctgaaaa gaacaacagc tcacacttcc cctataccga ctgagcgcca gcttccatca
      661 ccaaaagtca tttgcaaggg tctgtagtag atttcatcta tacggctctt gtctggcatg
      721 tttgttcccg caattcccgt aagcacacag ccatccattg agcgtaggct caagttgcta
      781 tagccactat atggacggta caccatctca tttcccagac gaatgccttt tactgtgctg
      841 tttcctgcgt cgcctgctgg ccccggaggc cccatcggtc ccggttcacc acgctcacct
      901 ttcggcccgg cagggccccg ttcaccggcg ggtcctcggg gaccggcagg ccctgcaggt
      961 cctgcatcac ctttagcgcc cgctgcacct gccgggccga caggaccagc taccccccga
     1021 ggcccctgag gtccccgctc tcccgccggt ccctgtgggc ctgtaggtcc tggctcgccc
     1081 ctgtcacctt taagtccctg tggaccgggc tgcccctgag gaccagcagg tccgggtttt
     1141 ccggtttcac ctttcgctcc cggttcgcct gccggacctc tctctccggc aggacccggt
     1201 ggtccctgta atccctgtgg ccctcgctca ccgggttcac ctttttcacc ggcagggcca
     1261 gcaggtccgg tatcaccctt tggtcccggt gggccgccgg ggtccccggg gtctcctttt
     1321 tcacctctgg ctccggtttc acctcgcggg cccgcaggac cggcaggccc ctgaataccg
     1381 cgctctcctt gtggcccaac aggtcctgtt gctccggctg gcccctgtgc accctgtggc
     1441 cccgtctcac cacgaggacc agcaggcccc gttgcccccg tcgggcctgc cggtccctgt
     1501 gcgccctgtg ctccacgttc tccggtttca cctttcagcc cctgtaatcc ctgtggtcca
     1561 cgctccccct gctcgccttt ctcaccccgc ggacctgccg ggccgacact accctgagga
     1621 cctctctcac cgcgctctcc tttcggaccc tgaggacctc tctgacccgg ctcacctttt
     1681 tcaccccggg ggccggtatc acctttgggc ccctggtcgc cctgaggtcc ccgcgcattc
     1741 tcagccatgc gccgggcttc ctctgcactg accgttgcag cctccgcacg ctcaagaatg
     1801 tctccggctg cctgctgagc cacccgggct gtttcagcat gttgttttgc atttttcgcg
     1861 tctgctgccg ctgccttttc agactcgcct gcccgggtcg ctgcttctgc agcttcactg
     1921 gctttttgtc cggcttcagc cgccctgctg gttgccgtct ttgcactgtc agatgcactc
     1981 tt
//
LOCUS       DAZGAN020000278         1963 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGAN020000278 DAZGAN020000000
VERSION     DAZGAN020000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.280"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(59..757)
                     /locus_tag="ACHKYO_RS25905"
                     /old_locus_tag="pgaptmp_005181"
     CDS             complement(59..757)
                     /locus_tag="ACHKYO_RS25905"
                     /old_locus_tag="pgaptmp_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4431817.1"
                     /translation="MQDIHEASLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQLSALTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(757..1086)
                     /locus_tag="ACHKYO_RS25910"
                     /old_locus_tag="pgaptmp_005182"
     CDS             complement(757..1086)
                     /locus_tag="ACHKYO_RS25910"
                     /old_locus_tag="pgaptmp_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431818.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(1083..>1963)
                     /locus_tag="ACHKYO_RS25915"
                     /old_locus_tag="pgaptmp_005183"
     CDS             complement(1083..>1963)
                     /locus_tag="ACHKYO_RS25915"
                     /old_locus_tag="pgaptmp_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4431819.1"
                     /translation="ADKFAQQQRAKRAAMDAKSRGLTDRQAEREATEQRLKEQYGDNP
                     LALNNVMSEQKKTWAAEDQLRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIA
                     QNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGG
                     TAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYA
                     EGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDE
                     LRLQLRDGGMLSGSGR"
BASE COUNT          385 a          709 c          444 g          425 t
ORIGIN      
        1 cgcacaccgc cgggcatgcg ccagaatcgc tgattcagtc tgtgtcataa aacgggattt
       61 actgcgaaag tttattaatg gaaaggaaac cgccgaaatt agccaccatg ccgcgcatct
      121 cacacccgcg catgcattta ctgcatctgt ccttccggat atccgtggtg gggttgtcga
      181 actcatccgc cactgccgga ccgttatacc cgcattcatc gccccggtaa tcccacatac
      241 aggtgttcgc cagcatgatg cgaccgggaa acagcgcacc gtccgtctcc gtcggtgtcg
      301 ccagcacaaa cgaggccgtc atggccgtca gcgctgataa ctgctccacc acccaccggt
      361 ccgtcagctc ctgctccggg tctgcctccg gattccctgc cacaaagttc accgcatcaa
      421 gaaaacgcgc atacacccgg cggcggacca ccgtggcacc caccaggctc tgcaaatcct
      481 ccgccatccc ggtgacaagg ccaaacagat tggacaccgt cagcgacggg cgggcactgc
      541 tgccctttcc gttcatctca aagccgctgc cctcaatcgg gtacgcctca tacttccgcc
      601 cctgccaggt caccggctcc cctttttcat tcagctcatt gcagaaaaaa taccgctcac
      661 cgccctgcac cgtcaggtcg atttcccaga gcaccacccg cggtgactgc tctgacttaa
      721 ccgactcgtt caggcttgct tcgtgaatat cctgcatcag ttcaccacct gcttaaactc
      781 cgcgctgaac tcaacgcgca acatcccgac ccgcgcagac cacccggcac aggtcacctt
      841 tatctgccgg tatgcatagg gtggcttcca caaaaatgcc ttccagcccc cgtgctctgc
      901 caggaacgct tccagatgcc gggcctcctc ccgggtcacg gaaagcgtca ccctgtatgt
      961 tttcaggtca gcattcagcc ctgccgccat acgctgtgag tacccgtcac caaaacgcac
     1021 ttcacgcacc gacggctgcg agttcacctc catatccggc ttcactttcc agcgaaaggt
     1081 tttcatcgcc cgctccctga taacataccg ccatcacgca actgcagccg gagttcatcc
     1141 tgcgccccct tgcgggccat gtcatacacc gccttcatca gctgcggccc cgcctgtccg
     1201 ttgataccgt cgttctgaat caccacgtga ttgttctgat taaaattaat accttccgcc
     1261 cgccgcatct gcgccggact tccggcacca cccacataac caccttccgc atagccgcgc
     1321 atcagacggt aaagattccc cacacctatc cggctggttg cctctttcgt gaaaacaaac
     1381 tccccgcggt gaactatccc cgcaggctca tatttgccgc ccgtgcccgt aaatccgccg
     1441 gtcgcaaaat gaaagttcgc cgcagcggcc tgaatggctg taccgcctga cgcggatgcg
     1501 ccgccaccaa cagccccgcc aatggcgctg ccgatactcc cgacaatccc caccattgcc
     1561 tgcttaagca gaatttctgt catcatggac agcacggaac gggtgaagct gcgccagttc
     1621 tgctcactgc cggtcagcat cgccgccata ttctgtgcaa taccatcaaa ggtctgcgtg
     1681 gctgcacttt ttacctgcga catactgtcc gtggcgctct cttcccactc actccagccg
     1741 gacttcaggc ctgccatcca gttcccgcga agctggtctt cagccgccca ggtctttttc
     1801 tgctctgaca tgacgttatt cagcgccagc ggattatcgc catactgttc cttcaggcgc
     1861 tgttccgtgg cttcccgttc tgcctgccgg tcagtcagcc cccggctttt cgcatccatg
     1921 gcggcccgtt ttgcccgttg ctgctgtgcg aatttatccg cct
//
LOCUS       DAZGAN020000279         1940 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGAN020000279 DAZGAN020000000
VERSION     DAZGAN020000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.281"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1475)
                     /locus_tag="ACHKYO_RS25920"
                     /old_locus_tag="pgaptmp_005184"
     CDS             complement(<1..1475)
                     /locus_tag="ACHKYO_RS25920"
                     /old_locus_tag="pgaptmp_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431820.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGVAQSVRGTGEMARGLGDALVNNPVKTLAGLSSFMTDVYMPP
                     SSTQKELGAPPSVGDAIKNIPDGTGKNAVTDAVGKGLKVTGKSVSDGAKASDEWLTDN
                     MSPGAVRALNTPMSEGYDDPAVWLTKGMNFVGAVAADVFAGGVAKKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQMFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPQLLAV
                     NAMAAKLGDAQLLNLAMRGTAKTVKSGIVRNATAQGAINAAQGGYSRYQENTTLRETA
                     GMDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRRQAAEETAMRDAETVQQDDAA
                     PQPESVDPVVQHRESMQGMNREQLLEQYADADMATEGDASAA"
     gene            complement(1545..>1940)
                     /locus_tag="ACHKYO_RS25925"
                     /old_locus_tag="pgaptmp_005185"
     CDS             complement(1545..>1940)
                     /locus_tag="ACHKYO_RS25925"
                     /old_locus_tag="pgaptmp_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431821.1"
                     /translation="MPKQQGLSLAKLQEVASNVAADAIKNGRNAQEEVDEFYATTGSP
                     QHQQKIQQQKIQQVITSWAGDDPDKQAFAREVASRQPEMLEPQNQKLLENGYANFLRI
                     KKARGEQARDESASSASQFIRGLKQNYAQ"
BASE COUNT          401 a          587 c          453 g          499 t
ORIGIN      
        1 gctgcggatg cgtcaccctc tgttgccata tccgcatcag catactgctc cagaagctgc
       61 tcgcgattca tcccctgcat ggattcacga tgctgcacca ccggatcaac agattctggt
      121 tgcggggctg cgtcgtcctg ctgcacggtt tcagcatcac gcatggcggt ttcttctgcg
      181 gcctgacgtc tgccacgata tccggcaacc gcaccggatg gagcccccat cgcagcacca
      241 aaggctgcac cttcgatcgt tgcgtcagcc acgccctccc atggcgatac atccattccg
      301 gcggtctcac gcaatgtcgt gttttcctga tagcgtgaat agccgccctg cgccgcatta
      361 atcgccccct gtgcagtggc atttctgaca atgccgcttt taacggtctt cgctgtgccc
      421 cgcatcgcca gattaagcaa ctgagcatcg cccagttttg ccgccatggc attcacagcc
      481 agcaactgag gatcggttgc cagttgcgca cgcacctcat cagccacacg ctcttttgcc
      541 aggtccattt tctggcgatc agtaagctgt gcgtgctgcg ggtcggcgtc aatggacaaa
      601 aacatttgct gaaatttcgg tgactgcgcc agctcagagt aatccgcatt aagaacagca
      661 tctgctgccg ccattgcact ctgcccctgt gcactggctg ttgaatgggt gatcaggccc
      721 gcctggaata aatccggcat ttttttatcg acagcttctg ctgccagtgc cgtggctctt
      781 tccggctgca tccctgccgc gatgtatttt ttctccagcc cggcggtcag catttttcgc
      841 aatgtgacat cacccacctt tttagccaca ccgccagcaa acacatctgc cgcaacagca
      901 ccgacaaaat tcatcccctt cgtcagccag actgcgggat catcatagcc ttcactcatt
      961 ggcgtattca gcgcacgcac agcacccggc gacatattat cggtcagcca ttcatccgag
     1021 gctttagcac catcactgac tgacttgcct gtgaccttca gacctttacc gacagcatca
     1081 gtcaccgcgt ttttccctgt tccatcggga atgtttttaa tcgcatctcc aacagaaggt
     1141 ggtgcgccca gttccttttg cgtggaggaa ggcggcatgt aaacatccgt catgaaagac
     1201 gacaacccgg caagcgtttt tacaggatta tttacaagtg catcaccgag tccacgggcc
     1261 atttcccctg ttccccggac ggactgggca acacctttac ccattgttgg caatacatcg
     1321 cccaggctga acgacgtact gttatccttc cagcgattcg gatcagagaa aaatgcttca
     1381 tagctgtcag tttcgccggg ttgctgaatg ttcaggctgt tacgattctg gttaccgagt
     1441 tgcgcctcag gacgctgttc ctctgaatag gccatacaga ctccataaaa aaacccggca
     1501 caatggccgg gcatcaggaa atggtgttaa tggaatatcg tgaattactg ggcgtaattc
     1561 tgtttcagtc cacggataaa ctgagatgca gatgaagcac tttcatctct ggcctgttcc
     1621 cccctggcct ttttaatacg gagaaaattc gcatacccgt tctccagcaa tttctgattc
     1681 tgaggctcca gcatttctgg ctgacgggaa gcaacttctc tggcgaatgc ctgcttatcc
     1741 ggatcatccc ccgcccagct ggtaatcact tgctgaattt tttgctgctg aattttctgc
     1801 tgatgttgcg gtgaaccagt tgtagcgtaa aactcatcca cctcctcctg agcattcctg
     1861 ccgttcttga tcgcgtccgc cgctacgttg ctggctactt cctgaagttt cgccagagaa
     1921 agtccctgct gcttcggcat
//
LOCUS       DAZGAN020000280         1940 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGAN020000280 DAZGAN020000000
VERSION     DAZGAN020000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.282"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1475)
                     /locus_tag="ACHKYO_RS25930"
                     /old_locus_tag="pgaptmp_005186"
     CDS             complement(<1..1475)
                     /locus_tag="ACHKYO_RS25930"
                     /old_locus_tag="pgaptmp_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431822.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKATGKAVSDGAEATDEWLIGK
                     MSPGAVRALNTPMTEGYDDSAVWVAKGVNLIGVLVPDMVVGGVAKKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENTALRETA
                     GMDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRRQAAEETAMRDAETVQQDDAA
                     PQPESVDPVAQQRESMQGMNREQLLEQYADADMATEGDASAA"
     gene            complement(1545..>1940)
                     /locus_tag="ACHKYO_RS25935"
                     /old_locus_tag="pgaptmp_005187"
     CDS             complement(1545..>1940)
                     /locus_tag="ACHKYO_RS25935"
                     /old_locus_tag="pgaptmp_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431823.1"
                     /translation="MPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDESASSASQFIRGLKQNYAQ"
BASE COUNT          401 a          600 c          449 g          490 t
ORIGIN      
        1 gctgcggatg cgtcaccctc tgttgccata tccgcatcag catactgctc cagaagctgc
       61 tcgcgattca tgccctgcat ggattcacgc tgctgcgcca ccggatcaac agattctggt
      121 tgcggggctg cgtcgtcctg ctgcacggtt tcagcatcac gcatggcggt ttcttctgcg
      181 gcctgacgtc tgccacgata tccggcaacc gcaccggatg gagcccccat cgcagcacca
      241 aaggctgcac cttcgatcgt tgcgtcagcc acgccctccc acggtgacac atccattccg
      301 gcggtctcac gcaatgccgt gttttcctga tagcgtgaat agccgccctg cgccgcatta
      361 atcgccccct gttccgtggc atttctgaca atgccgcttt taacggtctt cgctgtgcct
      421 cgtgtcacca gattaaacag ttgtgcgtca cccagttttg ccgccatggc attcacagcc
      481 agcaattcag gatcggttgc cagctgcgcg cgcacctcat cggcaacacg ctcttttgcc
      541 agatccattt tctggcgatc agtaagctgt gcgtgctgcg ggtcggcgtc aatggacaaa
      601 aacgtctgct gaaatttcgg tgactgcgcc agctcagagt aatccgcatt aagaacagca
      661 tctgctgccg ccattgcact ctgcccctgt gcactggctg tggaatgggt gatcaggccc
      721 gcctggaata aatccggcat ttttttatcg acagcttctg ctgccagtgc cgtggctctt
      781 tccggctgca tccctgccgc gatgtatttt ttctccagcc cggcggtcag catttttcgc
      841 agtgtgacat cacccacctt tttagccaca ccgccaacaa ccatatcagg tacaagcaca
      901 ccaatcaggt ttacaccctt cgccacccag accgcagaat catcatagcc ttcggtcatc
      961 ggcgtattca gcgcacgaac tgcacccggc gacatcttac cgatcagcca ttcatcagtg
     1021 gcttcagcac catcactgac agccttaccg gttgccttca gacctttacc gacagtatca
     1081 gtaaccgcgt ttttgccatc aggcagggta tcgatgacct catcagcccc cctgctgcca
     1141 ccggcaaaaa tatcctgcac agttgcgaca cccggcagcc ccatacggct gaactcattt
     1201 aaaatacgcg cccctgtttt taccgggctc tgaatcatcg catcaccgag tccacgggcc
     1261 atttcccctg ttccccggac ggactgggcg aaacctttac ccattgttgg caatacatcg
     1321 cccaggctga acgacgtact gttatccttc cagcgattcg gatcagagaa aaatgcttca
     1381 tagctgtcag tttcgccggg ttgctgaatg ttcaggctgt tacgattctg gttaccgagt
     1441 tgcgcctcag gacgctgttc ctctgaatag gccatacaga ctccataaaa aaacccggca
     1501 caatggccgg gcatcaggaa atggtattaa tggaatatcg tgaattactg ggcgtaattc
     1561 tgtttcagtc cacggataaa ctgagatgca gatgaagcac tttcatctct ggcctgttcc
     1621 cccctggcct tttgaatacg gagaaaattc gcatacccgt tctccagcaa tttctgattc
     1681 tgaggttcca gcatttcagg ctgacgggcc gctacatttc tggcaaatga cagcttatca
     1741 ggatcatctc ccgcccagtt gataacctgt tgctgaagtt tttgttgctg aattttctgt
     1801 tgatgcggtt gtgaaccagt cgcagcataa tattcatcca ccgcagcctg agcattaccg
     1861 ccattcttga tcgcgtccgc cgctacgttg ctggccccct tctgaagctc tttcagagaa
     1921 agtccctgct gcttcggcat
//
LOCUS       DAZGAN020000281         1937 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGAN020000281 DAZGAN020000000
VERSION     DAZGAN020000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.283"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            186..440
                     /gene="bssS"
                     /locus_tag="ACHKYO_RS25940"
                     /old_locus_tag="pgaptmp_005188"
     CDS             186..440
                     /gene="bssS"
                     /locus_tag="ACHKYO_RS25940"
                     /old_locus_tag="pgaptmp_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HGU4431824.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            555..1673
                     /gene="solA"
                     /locus_tag="ACHKYO_RS25945"
                     /old_locus_tag="pgaptmp_005189"
     CDS             555..1673
                     /gene="solA"
                     /locus_tag="ACHKYO_RS25945"
                     /old_locus_tag="pgaptmp_005189"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HGU4431825.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPDHDNTLFITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1721..1834
                     /locus_tag="ACHKYO_RS25950"
                     /old_locus_tag="pgaptmp_005190"
     CDS             1721..1834
                     /locus_tag="ACHKYO_RS25950"
                     /old_locus_tag="pgaptmp_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HGU4431826.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          512 a          464 c          451 g          510 t
ORIGIN      
        1 gctaaaaaaa tatgaatgac atgctgtttt attgttcata taaatcttaa aagcagaaat
       61 ttatctgttc ccccctttaa aaattcggtg aataccctta cttattggta tactgaagtt
      121 gcttcagata aaaacgcatt gaacctcgaa taacgttgtc tagtaacacg aattaggggg
      181 ccatgatgga aaaaaataat gaagtcattc agactcatcc gctcgtaggg tgggacatca
      241 gcaccgttga tagctatgat gcgctgatgt tgcgtttgca ctaccagacc ccaaataagt
      301 ccgagcagga agggactgaa gttggtcaga cgctctggtt aaccactgat gttgccagac
      361 aatttatttc gatattagaa gcaggaatcg ccaaaattga atccggtgat ttcccggtaa
      421 acgagtatcg gcgtcattaa catgacatga ccatccaccc aaaaggcacc tcaccggtgc
      481 ctttaccatt tctgggttgt atatctgcat tcagttaatt actctgctta aaccatgaga
      541 gcagagagaa aaaaatgaaa tacgatctca tcattattgg cagcggttcc gtaggcgctg
      601 ccgccgggta ttatgcaacc cgcgccggtt taaacgtgct aatgaccgac gcccatatgc
      661 caccgcatca acacggcagc caccacggcg atacgcgatt aattcgccat gcttatggtg
      721 aaggcgaaaa gtatgtccca ctggtactcc gcgcgcaaac actgtgggat gagctctccc
      781 gccacaacga agatgatccc atttttgtac gctctggtgt cattaacctc ggcccggctg
      841 actccgcatt tctcgccaac gtcgcccaca gcgccgaaca gtggcaactc aacgtcgaaa
      901 agctcgacgc gcaagggatt atggcccgct ggccagaaat acgcgtcccg gacaactaca
      961 tcggcttatt tgagactgat tccggttttt tgcgcagcga actggcgatt aaaacctgga
     1021 tccaactggc gaaggaagcg ggctgtgcgc agctgttcaa ctgcccggtc accgcaattc
     1081 gtcatgacga tgatggcgta actattgaaa cggctgacgg agagtatcag gcgaaaaaag
     1141 cgattgtctg cgcgggaaca tgggtaaaag acctgctccc ggagctgcct gtccagcccg
     1201 tacgcaaagt atttgcctgg tatcaggccg atggtcgcta tagcgtgaag aataaattcc
     1261 cggcgtttac cggtgaactg cccaatggcg atcaatatta tggttttccg gcagaaaacg
     1321 acgcgttgaa gattggcaaa cataacggag gccaggttat ccattcagcg gatgaacgtg
     1381 ttccgtttgc ggaagtggtc agcgatggtt cggaagcctt cccgttcttg cgcaatgtat
     1441 tgccgggtat cggttgctgc ctgtacggcg ctgcctgcac ctatgataat tcgcctgacg
     1501 aagattttat tatcgatacc ctacccgacc acgataatac actgttcatt accggcctga
     1561 gtgggcacgg ttttaaattt gcgtcagttt taggggaaat agctgccgat tttgcgcaag
     1621 acaaaaaaag cgattttgat ttaacgccat tcaggctttc ccgcttccaa taatcatcat
     1681 tacggcctcc agaatgaggc cgtttttaca ggagtattta atgcgtcggc tgttgcacta
     1741 tctcatcaat aatattcgcg agcatctgat gctttatctt tttctctggg gattgttggc
     1801 aattatggat ctgatctatg tgttttattt ttagttttcc gcactgacat aaactgacaa
     1861 atatttaatc tccctttcat ttagcgtgaa aaattgaatg agattattcc tgcttctaca
     1921 gcactcacca gacatct
//
LOCUS       DAZGAN020000282         1903 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGAN020000282 DAZGAN020000000
VERSION     DAZGAN020000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.284"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..431)
                     /locus_tag="ACHKYO_RS25955"
                     /old_locus_tag="pgaptmp_005191"
     CDS             complement(<1..431)
                     /locus_tag="ACHKYO_RS25955"
                     /old_locus_tag="pgaptmp_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGU4431827.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMTAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVHGGVSNGSLIESGGYQDVGSHNNFVGQANNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNS"
     gene            complement(496..615)
                     /locus_tag="ACHKYO_RS25960"
                     /old_locus_tag="pgaptmp_005192"
     CDS             complement(496..615)
                     /locus_tag="ACHKYO_RS25960"
                     /old_locus_tag="pgaptmp_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGU4431828.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(839..997)
                     /locus_tag="ACHKYO_RS25965"
                     /old_locus_tag="pgaptmp_005193"
                     /pseudo
     CDS             complement(839..997)
                     /locus_tag="ACHKYO_RS25965"
                     /old_locus_tag="pgaptmp_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1343..>1903)
                     /locus_tag="ACHKYO_RS25970"
                     /old_locus_tag="pgaptmp_005194"
     CDS             complement(1343..>1903)
                     /locus_tag="ACHKYO_RS25970"
                     /old_locus_tag="pgaptmp_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431829.1"
                     /translation="SALQAFSIFCSSTLIHNNIGFDFKLFPEVKLCGEHLETVFKYKN
                     GDDVREIAKINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNYDIQYVNWGTSLFDLDT
                     PCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGVAPFDDILFMDDDFDDS
                     SSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT          570 a          425 c          350 g          558 t
ORIGIN      
        1 tgtgaattac cacccttaat tgtcgttcca ttgctgatac cacctgtata aacgtcctga
       61 ttgccactgt cgattatcgt accggtggaa atgcccccgt catgaattgt ctgtcttcct
      121 ccgttaatgg ttgtattatt agcctgcccc acaaaattgt tatgacttcc tacatcttga
      181 tatccacccg attcaataag acttccatta gataccccgc catgaacatt ctgctggcca
      241 tggttgataa tatgagtgtt atttgttgta cctcgttcat ctactctttg gctgccatct
      301 acagtctcat cgtttaccac accaataaca tcaggagtga aggccgtcat cccaggcggg
      361 gcatatatca aggcagatat caataaggaa agtactgagc ggcgacaata atagggactg
      421 gtcctgttca taaatttcat cctctgaaaa gtgaatactg agtagcgttt aagcgacctt
      481 agctttgctg caacatcagc ccacaggcac cagaccaggg gattcatcct gaagagacag
      541 cgcaagtgta ttgtgttcac cgctcatcaa agacatcatg atgaaatgat gatattccgc
      601 ataagaatga ggcatttttt aaacgcagtg cgctgaagtg tggttggata aaaaagtcaa
      661 tccattcagg aaatacgggc gtattctttt ctttcgacaa tgaggccgtt ggcaaaataa
      721 aatgatttac ataatcgttt ctgataaata tcttctgctc acataaaaat cacacaataa
      781 ctttgagatc gcagattgtt ttacttttac agcattcgtc cccccattgt tgggcaaata
      841 tagattgggc cagagcacga aagttaatac cacgttcgca cagctcctcc aacagcacga
      901 caagatgcca catactgcgc cccagtcggt tcagtttaca gactagcagt gcgttccctg
      961 ccgatgatgt cctgaccagt tttttcagtc cggacctcct gccacggttt tacgttgaag
     1021 tggtaaccct gagcacgcag ttcttcagtc aggcgtggtg caccgtaacg ctgttattgc
     1081 tgggtaagat caaaaaactt tcaggcagct aaggaaagtt gaaccagaca ttagatgaaa
     1141 tatttcaacc aattacagca ccaattcagt cactgccagc ccaccaaata aatcaagggg
     1201 ttacatgaaa acgtagcccc tttttctttg gtagtgacac taaaatggat gtagtgtgaa
     1261 gaataatccc gtttactcaa tcaataatac atattgtttc aatctacgtt attatctctt
     1321 tgtaaaaata gccatttatt aatcattgaa aactgctttt agaacttgat acaacgggac
     1381 tagtcacaac aggactattc tcaacgggat catcctcaga ggaactatca tcaaagtcat
     1441 catccataaa taaaatatca tcgaatggtg ccacgcccgt gatgagtttt attttattat
     1501 tacgatcagt caagactcca cttaaaccgt tttcgctcac aggttttaat gatttttcat
     1561 tactcttgtt gtaagcaggc gcattaaaaa tacacggagt atcaagatca aacaatgacg
     1621 ttccccagtt cacatattga atatcatagt tactgaagtt ctgtccagaa aagaagcatc
     1681 ccttaaaatc caatccacct aaattatata aaccatcctc ttctttttgg agagtaatgt
     1741 taattttggc tatctcccgg acatcatcgc catttttata tttgaatacc gtttcaagat
     1801 gttccccaca cagtttgact tctggaaata atttgaaatc aaagcctata ttattatgta
     1861 tcaacgtaga tgaacaaaag atggagaaag cttgcagtgc tga
//
LOCUS       DAZGAN020000283         1879 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGAN020000283 DAZGAN020000000
VERSION     DAZGAN020000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.285"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..332)
                     /locus_tag="ACHKYO_RS25975"
                     /old_locus_tag="pgaptmp_005195"
     CDS             complement(<1..332)
                     /locus_tag="ACHKYO_RS25975"
                     /old_locus_tag="pgaptmp_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431830.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVNRPDNPDSNTLG
                     LRNALAIAG"
     gene            complement(335..991)
                     /locus_tag="ACHKYO_RS25980"
                     /old_locus_tag="pgaptmp_005196"
     CDS             complement(335..991)
                     /locus_tag="ACHKYO_RS25980"
                     /old_locus_tag="pgaptmp_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431831.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYQKILRPAEDNFM
                     EKVDDLNSEQQYENIAGTTNLGYQKQFGEARRELAGNLAQSGVDPSSGRFNAVMDANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            complement(1085..1438)
                     /locus_tag="ACHKYO_RS25985"
                     /old_locus_tag="pgaptmp_005197"
     CDS             complement(1085..1438)
                     /locus_tag="ACHKYO_RS25985"
                     /old_locus_tag="pgaptmp_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431832.1"
                     /translation="MQRVVRNSRNPEFMNEVKDACFKKQAFCFEAPDGFLVLRSVLSA
                     DGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHTARRGFIRLGKRLGFER
                     MPDDEDGFMVFRIAV"
     gene            complement(1574..>1879)
                     /locus_tag="ACHKYO_RS25990"
                     /old_locus_tag="pgaptmp_005198"
     CDS             complement(1574..>1879)
                     /locus_tag="ACHKYO_RS25990"
                     /old_locus_tag="pgaptmp_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HGU4431833.1"
                     /translation="DNRVSTLERSTNQQFRQLRDEVEKNRKRADAGIAGAMAMTAIPV
                     VEGKTYSFGMAASNYRDEQAIAAGMHFRTTENSAVRLNASWDTQNGSGVAAGMSVGW"
BASE COUNT          448 a          565 c          408 g          458 t
ORIGIN      
        1 ccagcaattg ccagggcatt acgtaacccc agcgtgttac tgtcgggatt atccgggcgg
       61 tttaccccgt atctcgccat ctggttatcc tgcgccatct gcgctgtacg gagactggaa
      121 gtggcaaggc ctcccacccg tgcaagctgt gcattcatca aactgttgtt ctccccaagg
      181 tcagccagcc ttgctacacg gggtaaatat ctggtgcgcc agtcgtcgta ttgctggcgg
      241 gtcagcgctg cggatgtctg ccagtccccc tgagggcggg ctgctccggt ataaccgtat
      301 cttgcaagtg tttcgtattt accgtactcc ataatcacag tctccagttc tgagcctgat
      361 gctgaatggc attagcgcca gtgccaggtg ttttagcacc gccacttcct ccgctaccgc
      421 cagccttatg catcgcatat gcacctgccg cacccagacc agcgccaaca agagaggctc
      481 gcccctgctg tttcgtaaac gccgcctgtg catccgattt agcttttgcc agactgctgt
      541 ctgccagaga gttaaaactc tgtaacgcat ccgccttctg accagaaccg agagcagcaa
      601 catcctgtag cccggcaaca tacttatctg cctgcgacac ctgcccccgt gtggttgtgt
      661 caatctgccc ggtcacctgg tcgctttgat ttgcatccat taccgcatta aaacgaccac
      721 tggatgggtc aacgccggac tgagcaagat tacccgccag ctcccttcgc gcttcaccaa
      781 actgtttctg ataaccaaga tttgttgtgc cagcgatatt ttcgtactgc tgctcactgt
      841 taaggtcatc gaccttttcc atgaagttat cttctgccgg acggaggatt ttttgataat
      901 cctgccaccc tttccaggcc acttcttcct gcgctatttg cgctgctgtt ggttttactt
      961 tggtatcacc accgccttta cttccaccca taatggcccc ctggataaca aaaaaccctg
     1021 ccggagcagg gtcagaatgt gaattacaat gttggtttaa acacaatgat gaaaactgta
     1081 gcagtcagac cgctatcctg aacaccatga agccatcctc atcatccggc attcgctcaa
     1141 agcccagacg ttttcccagc cggataaatc cccgccttgc cgtatgaaat tcagcccagc
     1201 gtccgccagc cagacgggtt aatgtcttca cctccggcag ataacgctca acgctgttac
     1261 tccccgtaca cacgcccagc aacaccagaa cataagggat accatcagca ctgagcacag
     1321 aacgcagcac cagaaagcca tcaggtgcct caaaacaaaa cgcctgcttt ttaaagcagg
     1381 cgtctttaac ttcattcata aattcagggt tgcgggaatt tctcaccaca cgctgcatat
     1441 accggagaat tttttcactc acactctcac cacaaaaaag aacaatgtga ctgtgctgtg
     1501 tcttcagcga cagaaggatg acggagaacg cttccgataa ccaacacggc tggcgttctc
     1561 cgcaaaaaat aaattaccag ccgacagaca tacccgcagc aacacctgaa ccgttctggg
     1621 tatcccatga agcattcaaa cgtacagcag aattttcagt ggtgcggaag tgcatacctg
     1681 ctgcgatagc ctgctcatca cggtagttac ttgcagccat accaaaggag tacgttttac
     1741 cctcaacaac aggaatggca gtcatcgcca ttgctcctgc aatacctgca tctgcacgtt
     1801 tacggttttt ctccacctca tcacgtaact gacggaattg ctgattagta ctacgctcca
     1861 gggtactgac ccggttatc
//
LOCUS       DAZGAN020000284         1826 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGAN020000284 DAZGAN020000000
VERSION     DAZGAN020000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.286"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            72..452
                     /locus_tag="ACHKYO_RS25995"
                     /old_locus_tag="pgaptmp_005199"
     CDS             72..452
                     /locus_tag="ACHKYO_RS25995"
                     /old_locus_tag="pgaptmp_005199"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4431834.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEAPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            449..796
                     /gene="tnpB"
                     /locus_tag="ACHKYO_RS26000"
                     /old_locus_tag="pgaptmp_005200"
     CDS             449..796
                     /gene="tnpB"
                     /locus_tag="ACHKYO_RS26000"
                     /old_locus_tag="pgaptmp_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4431835.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLPSLTML"
     gene            846..>1826
                     /locus_tag="ACHKYO_RS26005"
                     /old_locus_tag="pgaptmp_005201"
     CDS             846..>1826
                     /locus_tag="ACHKYO_RS26005"
                     /old_locus_tag="pgaptmp_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HGU4431836.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITE"
BASE COUNT          416 a          512 c          524 g          374 t
ORIGIN      
        1 tctggtcaga atctgacgaa ttagacaaag tggtgtccac caaataagta gtgggaacca
       61 aagtatcaga tatgcagaaa aatgtgactc ccggcaggcg aaaaggctgc cctaattatc
      121 ctcccgaatt taaacagcag ctcgttgctg cctcctgtga acccgggata tccatctcaa
      181 aacttgctct tgaaaatggc attaacgcca atctgttgtt caaatggcga caacaatggc
      241 gcgagggaaa actgctatta ccttcttcag agagccccca gctacttcct gtgactctcg
      301 atgccgctgc cgaacagcca gaatcgctcg cagaggcccc ggaaaccctc agtatcagct
      361 gtgaggtaac gttccggcac gggacgctcc gcttcaatgg caatgtcagc gaaaagctcc
      421 tgactctgct gatacaggaa ctgaagcgat gatcccgtta ccttccggga ccaaaatttg
      481 gctggttgcc ggtatcaccg atatgagaaa tggcttcaac ggcctggctg cgaaagtaca
      541 aacggcgctg aaagacgatc ccatgtccgg ccatgttttc attttccggg gccgcagcgg
      601 cagtcaggtt aaactgctgt ggtccaccgg tgacggactg tgcctcctga ccaaacggct
      661 ggagcgtggg cgcttcgcct ggccgtcagc ccgtgatggc aaagtgttcc ttacgcaggc
      721 gcagctggcg atgctgctgg aaggtatcga ctggcgacag cctaagcggc tgctgccctc
      781 cctgaccatg ctgtaaatct ctttatcctg gttgtcacag aataagcccg gtaaaatacg
      841 ggcttatgaa cgacatctct tctgacgaca tcttcctgct gaaacagcgc ctggccgaac
      901 aggaagcgct gatccacgcc ctgcaggaaa agctgagcaa ccgggagcgc gaaatagacc
      961 atctgcaggc gcagttggat aaactccgcc ggatgaactt cggcagtcgt tccgaaaaag
     1021 tctcccgccg tatcgcacaa atggaagccg atctgaaccg gcttcagaaa gagagcgata
     1081 cgctgactgg tagggtgtat gacccggcag tacagcgtcc gttgcgtcag acccgcaccc
     1141 gtaagccgtt ccctgaatca ctaccccgtg acgaaaagcg actgttgcct gcggcgccgt
     1201 gctgcccgaa ctgcggcggt tcactgagct atctgggcga ggataccgcc gaacagctgg
     1261 agttgatgcg tagcgccttc cgggttatcc ggacggtacg ggaaaaacat gcctgtactc
     1321 agtgcgatgc catcgtgcag gcacctgcac cttcgcggcc catcgagcgg ggtatcgccg
     1381 gaccggggct gctggcccgc gtgctgacct cgaagtatgc agagcacacc ccgctgtatc
     1441 gccagtcaga aatatacggc cggcaaggtg tggagctgag gcgttcactg ctgtcgggct
     1501 gggtggatgc atgctgccgg ctgctgtctc cgctggaaga ggcgcttcat ggctatgtca
     1561 tgactgacgg caaactccat gccgatgata ccccggtcca ggtactgctg ccgggtaata
     1621 agaagacgaa gaccgggcgg ttgtgggcgt atgttcgtga tgaccgcaat gccgggtcag
     1681 cgttggcacc tgcagtgtgg ttcgcttaca gcccggacag aaaaggcatc catccgcaga
     1741 ctcatcttgc ctgcttcagc ggtgtgctgc aagcggatgc gtacgccggg ttcaacgagc
     1801 tttatcgcaa tggtgggata acggaa
//
LOCUS       DAZGAN020000285         1825 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGAN020000285 DAZGAN020000000
VERSION     DAZGAN020000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.287"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            68..1813
                     /locus_tag="ACHKYO_RS26010"
                     /old_locus_tag="pgaptmp_005202"
     CDS             68..1813
                     /locus_tag="ACHKYO_RS26010"
                     /old_locus_tag="pgaptmp_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGU4431837.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMTVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
BASE COUNT          444 a          374 c          495 g          512 t
ORIGIN      
        1 ttcatcatct gtcaggggct gttttatggc taaactgata aatatatcag gtgttaccga
       61 ggagcggatg actgccgggg aaaagcgggt tgcatcccgt cttgaggcat ttatgagtaa
      121 tgagtgtctg gtctggtacg atattccggt gggacggaaa aaccgacatc ctgattttgt
      181 cattattgac ccggagaatg gtctggtatt tcttgaagtc aaggactgga ccgttcacac
      241 gttgcatcag gtaaatcacg aacaggtcat tcttgaaact aacggtattc ttaaatcaga
      301 aataaatccg ctgctacagg ttcgtcgtta tgcctgtgat accgtaaatg ctttacctgc
      361 agactcacgt ttgcgacaga atgatggtca gtataagggg cgtctgaatc tggcctgggc
      421 gtatggtgtt gtgtttaccc gtattacccg ccagcaactg aaggctctgg ctgggaatga
      481 tgaaaatgct gtggaaagga ttttcccttc agcgcagaca atctgtcagg atgatatgac
      541 tcagtctgtt ttacctgata tattccgcca aaaaattgcc gggatgttta cgatgggatt
      601 cagaacccgg gtgacaccac aggttagaga tattctgcgt gctcatctgt ttcctgaaat
      661 gaccgtcaga cagcactcac aaatcaaaat aatggatatt cagcaggaga ttctggccag
      721 aaatattggt gatgggcatc gtgttattca tggggtagcc ggctctggaa aaacaatgat
      781 tcttttgttc cgttgtcttt acttggctga aactacccct ggtaagattc tggtgctatg
      841 ttacaacatt actctggcca gctacctgcg ggaatgtatt gagtccaggg gactgaagtc
      901 acgggttacg gtattccatt ttcatagttg gtgcgcctca atgataaagc gccatggaat
      961 tcaggtgact gctggaggaa aggattaccc ggagaaatgc ttcagtgcgc tggaagatgc
     1021 cgtgaacagc ggaacaatta ctgatactgg atacgatgcc gtacttgtgg atgaggggca
     1081 tgattttgag agtcgctggc tggctctgat agcccgcctg tttgataacg cgtcccgttc
     1141 actgctcctg atgtacgatg acgcacagtc catttacaga cgggaacggg ctctgaactt
     1201 ttcgcttgcc agtgtgggga ttcaggcgca gggacgtact tccgtcctgc cggtaaatta
     1261 ccgtaatacg aaacgcattc tgcactttgc ttatgcattc tcccgtgagt attttgagaa
     1321 gcatcagaac cgtgaaatac cgtttgttca gccacaggcg ggcggtgaag agggcacaga
     1381 gccggaaatt ctgcgttgtg cttctgagag tgatgaggca gtgcaggttg tgggctggct
     1441 ggagaaacgc tatacactgt gcggtcactg gagtgatatg gctgttttgt gtcctgcaga
     1501 gttttcagtg aaacatctga aagaggtcat gacacaacgc gggatacctt atgccacctg
     1561 ctttgacagt gaaggtaaga agaaatattc gcgacgcaag gatgttgtgc atttactgac
     1621 ctaccagagc agtaagggac tggagtttcc gtatgttgcg gttatcaatg cttctttcgt
     1681 tcactcaggt gctgcggatg agtctgaagt tattccggcg ctttatgttg cgttcacccg
     1741 ggctacacgg gaattactgg tgacctgtta tcgtgaaaac agtatcagtc gtcatcttga
     1801 ggattttgta taagttgtgt tttat
//
LOCUS       DAZGAN020000286         1798 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGAN020000286 DAZGAN020000000
VERSION     DAZGAN020000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.288"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..300
                     /locus_tag="ACHKYO_RS26015"
                     /old_locus_tag="pgaptmp_005203"
     CDS             <1..300
                     /locus_tag="ACHKYO_RS26015"
                     /old_locus_tag="pgaptmp_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HGU4431838.1"
                     /translation="IYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHDWANYAS
                     ADYFRNIYNISPWRNIPYKKAVKKHEYKEKYKHLLYQKKFLDGVFTAIKYNVMKG"
     gene            309..1451
                     /locus_tag="ACHKYO_RS26020"
                     /old_locus_tag="pgaptmp_005204"
     CDS             309..1451
                     /locus_tag="ACHKYO_RS26020"
                     /old_locus_tag="pgaptmp_005204"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HGU4431839.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
     gene            complement(1487..>1798)
                     /locus_tag="ACHKYO_RS26025"
                     /old_locus_tag="pgaptmp_005205"
     CDS             complement(1487..>1798)
                     /locus_tag="ACHKYO_RS26025"
                     /old_locus_tag="pgaptmp_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGU4431840.1"
                     /translation="YNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGLVSLMMLYCA
                     ILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVYAGLLLAMKN
                     K"
BASE COUNT          608 a          295 c          330 g          565 t
ORIGIN      
        1 atttatacta taaagtcaga atttgaagaa aaaaatagtg aatattacac ccggtttatt
       61 aatgatgaca ctgtcttcat acattatact ggtataacta agccatggca tgattgggcg
      121 aactacgcct ctgcagatta ttttcgtaat atttataata tatcaccatg gagaaatata
      181 ccttataaaa aagctgttaa aaaacatgag tacaaagaaa aatataaaca cttgctttac
      241 cagaaaaaat ttctcgatgg tgtttttaca gcaattaaat ataatgttat gaaaggttaa
      301 tatacgagat ggttgataaa ataatattta cggttactcc tattttttca attcctcctc
      361 gcggagcggc tgctgttgaa acgtggatgt atcaggttgc tcagcgtacc aattttccaa
      421 atcgtattgt ttgtatcaaa aatccaggat actcaaatta cacctttgtt aatgataatt
      481 gtagtattca tagggttgga ttcagtcgca tatataagcg gttatttcaa aaatggacaa
      541 gacttgatcc tcttccatat tcacagagaa tattgaatat tgcacatgat tttcctataa
      601 cgaaagaaag tgttattgta gttcataata gcatgaagct atacactcaa attcgtaagc
      661 gagctcctca ggcaagagtg gttatacata tgcataatgc atttgaacca aaattgcttg
      721 agcaaaatgt taaaatgata gtgcctagtt tatacttgaa aaagtattac caatcttatt
      781 tagcaaatgc agatattgag attgtaccaa atggaattga tttagaaacc tatcaatcta
      841 attttcagcc gatcactaga agcgaactta atatctcccc tgaagagaaa attatttttt
      901 atgctgggcg tatcgtccct gacaaaggca tattgctgtt gatgcaatca ttcgaaaaat
      961 tggccgctgc tcacaaaaat ttaaaattag ttgtgattgg tgattataca gaaatgagta
     1021 aaagtgacaa aggtgcatat caacgcaatg tcagggaaat tgcaaaacgt ttgaaggatc
     1081 gttgcattat gttaggaagc ataccgcctg aaaagatgca ttgttattac ccactggcag
     1141 atttagtcgt cattccttcc cagttccagg aaccattttg catggtggca attgaagcta
     1201 tgggtgctgg taagcctgtt ttagtgagta cgcgtggggg gatgacggaa tttgtcaaag
     1261 aaaatactac gggattccat ttaaaagaac ctatgactgc agactccata agcagcgata
     1321 ttttaaaaac gttagcgaat cctgaattaa cagcagttgc aaagcaaggt caggactttg
     1381 tgtttgaaca ctacagttgg gatggcgtaa ctcaaagatt cgaagaggtt attcataatt
     1441 ggtttgaata aataaaaagg ccgcattatg cagccttttt tattttttac ttgtttttca
     1501 tcgctaataa taagccggcg taaacgccta ataaatttgg ttcaatttgt tctacgtttc
     1561 cacggataac aaaataacct ataaaagata gtaagattat ataatatgca ttatatggtg
     1621 agcgatattt attcttgacg ccatttttta tacactcttt caatattgca cagtatagca
     1681 tcataagact taccagccct aataaaccag tgccaaacca gatgaatagc gcaaaattat
     1741 gtggccctat tgattgtcta aaggtccatt ctggataatc aatgacacgt ttattata
//
LOCUS       DAZGAN020000287         1753 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGAN020000287 DAZGAN020000000
VERSION     DAZGAN020000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.289"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..189)
                     /locus_tag="ACHKYO_RS26030"
                     /old_locus_tag="pgaptmp_005206"
     CDS             complement(1..189)
                     /locus_tag="ACHKYO_RS26030"
                     /old_locus_tag="pgaptmp_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431841.1"
                     /translation="MDNRVSVFEKGESARRSRTTERSESVSEEAEKRLDEHFLLTHLC
                     GISHPAWCTFHTIRTVAA"
     gene            562..706
                     /locus_tag="ACHKYO_RS26035"
                     /old_locus_tag="pgaptmp_005207"
                     /pseudo
     CDS             562..706
                     /locus_tag="ACHKYO_RS26035"
                     /old_locus_tag="pgaptmp_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314450.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1297..1596
                     /locus_tag="ACHKYO_RS26040"
                     /old_locus_tag="pgaptmp_005208"
     CDS             1297..1596
                     /locus_tag="ACHKYO_RS26040"
                     /old_locus_tag="pgaptmp_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin"
                     /protein_id="HGU4431842.1"
                     /translation="MREISQRDLYQVLGAGNTDPNTQLLKDLANNMAWGAALGAKGGL
                     GTAAVGAGGAAVQTVVQGLINHGPVSVPVPVLIGPSWNGSGSGYNSATSSANGGS"
BASE COUNT          468 a          433 c          406 g          446 t
ORIGIN      
        1 tcatgcggca acagtgcgga ttgtatgaaa agtgcaccat gccgggtgtg aaatgccgca
       61 cagatgcgta aggagaaaat gctcgtccag gcgcttttcc gcttcctcgc tcactgactc
      121 gctgcgctcg gtcgttcggc tgcggcgagc ggactctcct ttctcaaaaa cgctaaccct
      181 gttatccaca gaacccgggg ataaaccaag aaagaacatg tgcgcaaaaa acgaaaaccc
      241 cggaaaaggc cgcgccgggg gcgctttttc cataggctcc gcccccctga cggacttcac
      301 aaaacagacg ctttttcggt gcatctgtgg gagccttacg gctcaaccat gaaatccgcc
      361 agcggtggcg aaacccgacg tggcttaaag atcatcaccg tttccggtga atcgctccct
      421 cctgcgcccc cacgttcccg ccatatcgat taccggatac ctttccgcct ttctcccttg
      481 cgggacacgt ggcgctttct catagctcac gtcttctggt atctcggggc gacttaggca
      541 gttcgctccg ggccgggctg tatgcaggaa ccccccgttc agcccgactg ctgcgccttc
      601 tccggtaact gttctcttag gtccaacccg gaaaagacac gacttaacgc cactggcagc
      661 agccattggt aaccgggtta gcagagcgag atttataagt tggtaacaga gttcttgaag
      721 tatcagtctg attttgacta cactagaagg accatttagg tacctgtgct ccactaaagc
      781 cagttaccac cgccgattag aagaggtcga agacttctga tcggacggaa gaacccgcca
      841 caaggcgggt tttttcgttt acaggcagca gattacgcat agaaaaaaag gatctcaaga
      901 gctcctttga tctttttcca aatagccccg tcctccccct cctaaaaaag cgatagataa
      961 gaacaaaaat tcggttaata atcatactaa tgcatctgtt ttggatatta aggataatac
     1021 gcatgacatt actttcattt gcattttccc ctgttttctt gtcagtcatg gcgttctgta
     1081 ttattttgcg cagccgctca tatccccaaa aaacacacaa cagagttatc gtgttgatat
     1141 taatgtgttt tttcatttgt tttttatatc cattaacaaa ggtgtactta actggaagtt
     1201 acaatatatt tgacaatttc tacctctttt gttttttgtc ttcattagta gcaattgcaa
     1261 taaacatagg aatacttaca gttataggag taaagaatga gagagattag tcaaagagac
     1321 ttatatcagg ttcttggcgc aggcaacacc gacccaaata ctcaactgct aaaagatctt
     1381 gctaataaca tggcatgggg tgcagcttta ggtgcaaaag gagggcttgg cacggcagct
     1441 gtgggtgctg gtggagcagc ggtacaaaca gtagtacaag gactaattaa ccatggacct
     1501 gtaagtgtcc ctgtccccgt actcattgga ccaagctgga atggtagtgg tagtggttat
     1561 aacagcgcaa catctagcgc caatggtggc agttaatctt atttggagct ccaaaccgta
     1621 attcctttga caacaaaaag ttggcgctca gcggtctggt acgtaagagc gctgttaacc
     1681 taacagcaaa gaaattgcag cttaaaaccg gaagaaggga cggaacccag ggaagggatt
     1741 taccataatc cct
//
LOCUS       DAZGAN020000288         1707 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGAN020000288 DAZGAN020000000
VERSION     DAZGAN020000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.290"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(130..357)
                     /gene="mchB"
                     /locus_tag="ACHKYO_RS26045"
                     /old_locus_tag="pgaptmp_005209"
     CDS             complement(130..357)
                     /gene="mchB"
                     /locus_tag="ACHKYO_RS26045"
                     /old_locus_tag="pgaptmp_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HGU4431843.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            complement(374..583)
                     /gene="mchI"
                     /locus_tag="ACHKYO_RS26050"
                     /old_locus_tag="pgaptmp_005210"
     CDS             complement(374..583)
                     /gene="mchI"
                     /locus_tag="ACHKYO_RS26050"
                     /old_locus_tag="pgaptmp_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HGU4431844.1"
                     /translation="MSYKKLYQLTAIFSLPLTILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRKILVWSGWKNGS"
     gene            1397..>1707
                     /locus_tag="ACHKYO_RS26055"
                     /old_locus_tag="pgaptmp_005211"
     CDS             1397..>1707
                     /locus_tag="ACHKYO_RS26055"
                     /old_locus_tag="pgaptmp_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431845.1"
                     /translation="MYLTKKIIISMMFILPSAAFSSDPPPLQQSLEKTTYFSIGMNGF
                     IGYQSEGEKLYTHILTLDNPEEIFKNIIKNRMSTKESKIYAACGLYYLNVENIESLFT
                     E"
BASE COUNT          603 a          327 c          300 g          477 t
ORIGIN      
        1 atatcttcat cagtaaagtg ttgaacataa aaacacactt ataaatggat cttctatctg
       61 ctttaaacga aagggatata ttttacaggc actaccgaaa aagtgcccgt aaaataaata
      121 aaattataat tagctaccgc caccagcaga agaactggca ctaccacttc ccacggtcga
      181 gccaattgct gttgtcaaac cggcagatac ggctccaact acaaccccaa gtggaccacc
      241 aggtattccg gcgagagctc caacaatagc tgcagcacct gcggcattag ctgaagtcgc
      301 tggcgcacct cccgccccgg aaatatatct taactgtgat tctgttattt ctcgcatata
      361 aactccatca tatttaactt ccgtttttcc agcctgacca gaccagaatc tttcgaatcc
      421 catgaatcag ctcaataaaa ccaagaaata ttataaagct tagaaaaacg tcaacataag
      481 aattcccttc gccaacaatc cgaagggatg aaagtgaaac caataagata gtaagaggta
      541 aactgaatat agccgtcaat tggtacagtt ttttataact cattaatacc tacctgttgc
      601 tttattttca ttatgaaaag ttaccaaatc ttagcgcttc agtcagggaa gtcaggtcat
      661 cgataaagaa tattccagta gagtggcgct catgatcaaa gacaacagca aagttgatga
      721 cagtgctgac cgactgtcat ttacagtaac cctgtttgta gcaaattcca tcttacctga
      781 cctcacccag acaaagacaa aagagaatga ttgttattta cttttcaaaa aaaagtgtca
      841 tattgtccat ataaagtcaa gaggtatttt acgttatgta actgatgcaa ttgttagtca
      901 ggatctcatg gttgtcgctc atgtataacc tgagacttga acgtacacta atagcactga
      961 cggtcataca tcgggaaatg tgaggaggca taagaatgaa ttgtgataat aatcacagaa
     1021 atgaagaatt cattgttacc tttgataaag gcaacaagca agacaattca agacgaaaac
     1081 acgataattt tcctatagag gtagaatcct ccgtagagca ggagacacac tgtatcacaa
     1141 ataataagtc ggcttccggt atagtaacac atgactatga tgccgattat atttgtggtt
     1201 gtggtgaaat tatgtgtcct ggttgcggtc atgacctata acaaacattt ttgcaaactg
     1261 atacctgatc aggtacaagc ctgttgatta ttatgctgaa tattccatgt tataaaacca
     1321 tcgatataaa cattaacaag aaaaaacata tattgtttat ctgaacattt atcgatggaa
     1381 aaccaaaggg gaaagtatgt atcttacgaa aaagattata ataagtatga tgtttatatt
     1441 accatctgct gcattttcat cagatccacc tccccttcaa caatcgttag aaaaaacaac
     1501 ctatttttct ataggtatga atgggtttat aggctatcag agcgaagggg aaaaattata
     1561 cacacacatt cttacattag ataatcccga agagatattt aaaaatataa taaaaaatag
     1621 aatgtcaact aaggagtcta aaatttatgc tgcttgtggg ctatattatt taaacgtaga
     1681 aaatatagag tcattgttta ctgaaaa
//
LOCUS       DAZGAN020000289         1702 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGAN020000289 DAZGAN020000000
VERSION     DAZGAN020000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.291"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1101
                     /locus_tag="ACHKYO_RS26060"
                     /old_locus_tag="pgaptmp_005212"
     CDS             <1..1101
                     /locus_tag="ACHKYO_RS26060"
                     /old_locus_tag="pgaptmp_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4431846.1"
                     /translation="LSGNFKPDWRWDVSKETFTKEKFDSYVKPVFSKIDFYKQCGVIN
                     PQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHEEMASSAYKHKNN
                     KVLYDLLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSM
                     RNMEGNKDSRINKNVVVLAGNHEINFNGNYTARLANHKLSAGDTYNLIKTLDVCNYDS
                     ERQVLTSHHGIIRDEEKKCYCLGALQVPFNQMKNPTDPEELANIFNKKHKEHMDDRFI
                     HLIRSNAIASTPVYDNYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKI
                     DNYTMGLNSWKIAPNERGDKKGVPGLSCFQPH"
     gene            1363..>1702
                     /locus_tag="ACHKYO_RS26065"
                     /old_locus_tag="pgaptmp_005213"
     CDS             1363..>1702
                     /locus_tag="ACHKYO_RS26065"
                     /old_locus_tag="pgaptmp_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431847.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTIN"
BASE COUNT          586 a          267 c          330 g          519 t
ORIGIN      
        1 cttagtggta atttcaaacc tgactggcgt tgggatgtct ctaaagagac gtttactaag
       61 gaaaagtttg atagctatgt taaacctgtg ttttcaaaaa tagacttcta taaacagtgt
      121 ggtgtgatta acccacaaaa tgccaacacg gcttattttg gcgatacaga tggaagagta
      181 ggcgctgtac tttatgcatt gctcgtttca gggcatatag gcatcaggga aaagggttgg
      241 agtttattat gtgaattatt aaaacacgaa gagatggctt catctgcata caaacataaa
      301 aataataaag tgctctacga tttattaaat acgagagaca tgatcttgaa tgagttgcat
      361 caacatgttt ttttaaagga cgatgcgata accccttgta tcttccttgg ggatcataca
      421 ggcgatagat ttagtactat ttttggtgat aaatatatcc ttactttgct aaattctatg
      481 cgaaatatgg agggtaataa agatagccgt attaataaaa atgtcgtcgt tctggctgga
      541 aatcatgaaa taaattttaa tggtaactat actgcacgac tagcaaatca taaactctct
      601 gccggtgata cgtataactt aattaaaacg ctcgatgttt gtaattatga ctcagaaagg
      661 caagttttaa ccagtcatca cggaataata agagatgaag aaaaaaaatg ttattgtctc
      721 ggtgctttgc aggttccatt taatcagatg aaaaatccta ctgatcctga ggaattagcc
      781 aatattttta ataaaaaaca taaagaacac atggatgatc gttttatcca tctaattaga
      841 agcaatgcta tagcatccac acctgtatat gacaattatt ttaataacac tactgctttc
      901 agaccaaaac ctgaagatat cttcaaatgt ggacagacgc tcaaaaaaac taaacagaaa
      961 tatggccatt atggattagg ggtagatcag catcaaaaaa ttgataatta taccatgggg
     1021 ctaaattcct ggaagatagc tcctaacgaa cgtggtgata aaaaaggtgt cccagggctt
     1081 agttgttttc aaccacatta aaactccatg agtgctgtgg tatatctttt taatcctgcc
     1141 agtatttcat aggttttctt aatcgattgt agaggcatgt tctgatgacg cctctacaat
     1201 ctttagggac tttctctgct atgagataag aaatttcata ttatgcttta aggctgaatg
     1261 atttgtgcaa aatataatta tttttccatc gaacatgagg tgtatgtcat aagattaaat
     1321 gttaactatg cattcctcgc ggtgaattat atgaggtaaa agatgaaaat tggaactgtg
     1381 gcaggcacta acgacagtac aacgacaatc gcaacaaatg atatggtgca agagcatgtt
     1441 actaacttca ctaaagaact gtttgggtat attgcaaatg gtataggtga tgatatatca
     1501 agtattgcca ggacaatgct cggtgaagtt gtggaaaaaa ttgatgattg gcaaatagaa
     1561 cgtttccaac aatctattcg agatgacaaa atatcattta ccatacaaac gaatcattca
     1621 gaaaaatata gtatgctatc ggacatgcgc gctcatattc tacgtaggga taataattat
     1681 cagtttattg tgacaattaa ta
//
LOCUS       DAZGAN020000290         1699 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGAN020000290 DAZGAN020000000
VERSION     DAZGAN020000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.292"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..275
                     /locus_tag="ACHKYO_RS26070"
                     /old_locus_tag="pgaptmp_005214"
     CDS             <1..275
                     /locus_tag="ACHKYO_RS26070"
                     /old_locus_tag="pgaptmp_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936711.1"
                     /note="participates in the regulation of osmotic pressure
                     changes within the cel; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HGU4431848.1"
                     /translation="VMSEERVLKNREITVRLNEPGESSMNFVVRVWSRRDDLQSVYWD
                     VLERIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSRSVSSRQ"
     gene            401..1621
                     /locus_tag="ACHKYO_RS26075"
                     /old_locus_tag="pgaptmp_005215"
     CDS             401..1621
                     /locus_tag="ACHKYO_RS26075"
                     /old_locus_tag="pgaptmp_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HGU4431849.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
BASE COUNT          444 a          310 c          456 g          489 t
ORIGIN      
        1 ttgtgatgtc agaagagagg gttctgaaga acagggaaat cactgttcgc ctgaatgagc
       61 ctggggagtc atccatgaat tttgtagtac gggtatggag caggcgtgat gaccttcagt
      121 ctgtatactg ggatgtactg gagagaatta agcgggagtt tgattcagaa gggataagtt
      181 ttccatatcc acaaatggat atccatcttg ttcgctccgg gaagcaggca gagtcggtaa
      241 cccataacag tcgctcagtc agttccagac agtaaaatga aaagcgcatt ttatttgtga
      301 aagatgaaca gcctgaatct gtcaggctgt tttcattgtg tttttatggt catttttggt
      361 atatgtactg tatataatgt catctgtaca aggatataaa atgccaaaga aaataatgac
      421 agggattgat gtttttgacg ctgccatgga gcgtattgaa tgggtgtttg atacgttttc
      481 atcagtttgt ctttcttttt ccggcggaaa agactccact gtcctgtttc acctgatggc
      541 gaatattgcc cgccgaaaaa aacgccgttt ttctgtactg tttgttgact gggaggctca
      601 gtatcagtgt accattgagc atgtcaccag gttgagggaa atgtacgatg atatcacaga
      661 tacttttttc tgggtggcgc tgcctctgac gaccgttaat ggagtgtctc aggtacagcc
      721 tgagtgggta tgttgggagg acggggctga atgggttcgc cagccaccag aagatgccat
      781 aaccagagcg tctttttttc ctttttatca gaatgcgatg acatttgaag agtttattcc
      841 ggcatttaat cattggtttg cccgggaaaa agggatggtt gctgcgattc ttactggtgt
      901 tcgcgctgat gagtcactga atcgttttat gggactggta tccggacgta agctccgtta
      961 tgcggaggat aaaccctgga ccacggcatc ggctgaggga ttttactaca ccatgtatcc
     1021 tttgtacgac tggaaaacaa gagatgtatg gatttaccat gcgagaacac gtgctgccta
     1081 taactctctt tatgacctga tgtacagggc tggagttcct cttcgcaata tgcgggtgtg
     1141 tgagcctttt ggaccggagc aacgtaaggg gttgtggctt tatcatgtac tggagccgga
     1201 tacatggggc agaatgtgtg ctcgcgtatc aggtgctgta gggggagcgc tctatgcaaa
     1261 tgaaagtggt gcttattttg ctttgaggaa acagataaca aaaccagttc accatacatg
     1321 gcagagttat gcaatgtttc tgctggagtc aatgcctgag aaaacggcgg agcattacaa
     1381 gaataagatt gctgtttatc ttcggtggtg gcagtctcat ggatggccgg atggcatccc
     1441 tgatgagcag gataatgacc agggatgtaa agatattccc tcctggcgtc gcatctgtaa
     1501 gacgttactg aaaaatgatt tctggtgtcg gatgctggcg tttagtccga ataagccccg
     1561 acattatgag cgctacctgg ccagaatgcg tgaaaaaagg aaagagtggg gaatattgta
     1621 aattatattt aaacatcaga aatatgacgg ttaatccagg atagtagcta aaaaaataag
     1681 attccttgct gatttatcc
//
LOCUS       DAZGAN020000291         1680 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGAN020000291 DAZGAN020000000
VERSION     DAZGAN020000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.293"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..755)
                     /locus_tag="ACHKYO_RS26080"
                     /old_locus_tag="pgaptmp_005216"
     CDS             complement(<1..755)
                     /locus_tag="ACHKYO_RS26080"
                     /old_locus_tag="pgaptmp_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431850.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPGGKCIITGDFQKELAYLQNVIIYNDSSLRMDFFGYNALIIQRSD
                     NTCELTINEPLKNQEISTGNINVNFPLKDIYNEIRRLNLVFSCGTGGIVDLSSLDLRN
                     IDLELYDFTDKHMANAILNPFKLDDTDFTNANMFQVNFVSSKQNTTISWDYLLKITPV
                     LTSISDMYSEEKIKLVESCLNELGDITEEQLKI"
     gene            complement(777..1316)
                     /gene="yhaB"
                     /locus_tag="ACHKYO_RS26085"
                     /old_locus_tag="pgaptmp_005217"
     CDS             complement(777..1316)
                     /gene="yhaB"
                     /locus_tag="ACHKYO_RS26085"
                     /old_locus_tag="pgaptmp_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HGU4431851.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1572..>1680)
                     /locus_tag="ACHKYO_RS26090"
                     /old_locus_tag="pgaptmp_005218"
     CDS             complement(1572..>1680)
                     /locus_tag="ACHKYO_RS26090"
                     /old_locus_tag="pgaptmp_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HGU4431852.1"
                     /translation="YFKISNTGIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          524 a          287 c          300 g          569 t
ORIGIN      
        1 atgattttta attgttcttc ggtaatatct ccaagctcat ttaaacaact ttcgacaagc
       61 ttaatttttt cttcagaata catatcgcta atgcttgtta gaacaggcgt tatttttagt
      121 aaataatccc aggagattgt ggtgttttgt tttgaactaa cgaaatttac ctgaaacatg
      181 ttggcattag taaaatctgt gtcatccaat ttaaaaggat ttaaaattgc attggccata
      241 tgtttatctg taaaatcata aagttctaaa tcgatattcc gtaagtccag agaggataga
      301 tcaactatac ctccagtccc acaactaaaa actaagttca acctcctgat ttcattgtaa
      361 atgtctttca atgggaaatt aacattgata ttgcctgtgg atatttcctg gttttttaac
      421 ggttcattaa ttgtaagttc acaggtattg tctgatcgtt gaataattag ggcgttataa
      481 ccaaaaaaat ccatacgaag agacgaatca ttataaataa ttacattttg taagtacgct
      541 aattcttttt gaaaatcacc agtgataatg cattttccac ctggttgtaa cgcgttaagt
      601 aaagtgacca tttgaataga ggtctgtttt agtttttcta tacctggtgc tagtgcttcg
      661 aggttattaa ctatggggct ttcaggaaga tcattcattt ttcgacggac taaatcacta
      721 ctaatatcat taccaattga tgaaacaggg aacattattt tatcctttat aaataatcaa
      781 taaaaatcat tattaaaatg atcgggtgaa aaaaagagaa tttgccaaca atctgaagta
      841 atgctggttt tattaaagtg cgcaggaggt gttaagatgg cttcttttcg ggtgaatttt
      901 gcacaatact tagaaatcgt ctccttaata cattccagct cttttttatt gacggctgag
      961 caagtacatt gttcaccaaa aggtgcaaaa gtgcctacat ctaatgtttg tatgatataa
     1021 tcgtcatgtt ctcctgtaga aatatctatg atgaatggac tcatattttc cggttggatt
     1081 cggatcatga cagaaggaac atcgccttga tagaaattca atgcagtaat tgcagataat
     1141 actctccgca gttcattact tatactgtaa attttattaa cgtcattggt ggagacaatt
     1201 tctgcaaact ttctttttac cgtccaataa tcaatatttc tattgtgatt gttaaccact
     1261 gcgtgcattg gcgggattga agggtgaaaa atatgtgcaa ttgggaagcc tttcattgat
     1321 gcctgccttg tcgtcatgac aaacaataat agatgcttat tttcctcgca gggttctttc
     1381 gcactatcaa aaaacgctca tttacccggc gttacagtaa acgaagcaac attctgtggc
     1441 ctacgacgat caaaagctct gtgaaaattt cccttgttgt ctttgtctga ccttatcgcg
     1501 gcgatcagca attattcgtt ttgtgatcct caacacaaaa tgctattcgt cgcctatgag
     1561 gggagaagaa tttatggaaa acgattgcat tcacaaaatg acgtcctgtc agaagagaaa
     1621 aagttgacgt taatcaattg ttcgagatga tcaatacctg tattcgaaat tttaaaatat
//
LOCUS       DAZGAN020000292         1664 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGAN020000292 DAZGAN020000000
VERSION     DAZGAN020000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.294"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>1664)
                     /locus_tag="ACHKYO_RS26095"
                     /old_locus_tag="pgaptmp_005219"
     CDS             complement(<1..>1664)
                     /locus_tag="ACHKYO_RS26095"
                     /old_locus_tag="pgaptmp_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HGU4431853.1"
                     /translation="DGHRVDWMVNGLAGDALHKIGKGILVVAGSGENPGALNTGDGTV
                     ILAQKADAAGRVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDMAF
                     NRLAAADEGAVLTSRARPATVRLDFSQSGQKAVMWHGHFTGNLSVLNNTSSAVDFIMD
                     GGADMSGSFTQQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDWES
                     RTFSIGQLKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKG
                     TSAVGGASGSSTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRLTS
                     SALTVSDGGRLHVKGGLETDGGVTLDRGTLLVDGGSVRNDVYERLLAWSEERGGLNGS
                     GEYDFMTGAAGLLRGYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGEF
                     HTLTAGSMDISDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIPL
                     VTAPAGTGADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQP"
BASE COUNT          367 a          569 c          367 g          361 t
ORIGIN      
        1 ccggctgcac aacattcagc tgccaggccg tgccgcccgt cccctgttca gccctgacaa
       61 caggtgtgat atgactgaag ccggtatcac gcgttttcac actgaacaca tccgcaccgg
      121 taccggccgg cgccgtcacc agagggatat tcagcgcctt ctgcccgtca gaacgctcag
      181 taaaatcaac caacaacagg ttattcttac cccgcagcga ttctgtcacc cgaagctggt
      241 ctgagtggtt aaggtccgtc cgcatcacca gaacactgtc gctgatatcc atactgcctg
      301 ccgtcagggt atggaactcc ccgcccggcc tgctgaaata cagagcactt cccgatgact
      361 caagatgctt cacggatgag tttcccgtca tcatccaggc cgcattctgc agattcacat
      421 tacctgcact ccctctgaca taaccccgca ggagaccagc tgcccccgtc ataaaatcat
      481 actcaccgct tccgttcagg ccgccacgct cctcagacca ggccagcaga cgttcatata
      541 catcattcct gacacttccc ccgtccacca aaagggttcc tctgtccagt gtcacaccac
      601 catctgtctc cagtcccccc ttcacatgta aacgcccgcc gtcactgaca gtaagtgcag
      661 aagaggtcag gcgggaattc ccttccagac gcacattctc tgaactcaca gctatccggc
      721 tgtccgatgc cgttatactg ccagtaaaat ggtcgcggac cgtcagggca gaatgacgca
      781 tattcacatt cccgcgaaat gtactgctac cggatgcacc gcccacggca gacgtcccct
      841 taaccggcgc actgctgctc cccgtgccgt ccttcatatc cagatatagc gagtcacttc
      901 ccagcaccat ggtggcatta tcagcatcaa tatctcccgt cagtgtcgca ttgcgggaaa
      961 gactgaatgc cgcccccttc agtttcagtt gaccgatgct gaacgttctg ctctcccagt
     1021 ccttctgagc aaatgatacc ggctgcgtca gaacagaatt atcaccctgc tttcgcaatg
     1081 cagtcgcaac cgcctcactg ctgacagcat gaacaaccgg atgcccctgg atatacagtc
     1141 ccccgccctg ctgtgtaaaa ctgccggaca tatccgctcc gccatccatg ataaaatcca
     1201 ccgcagatga cgtgttgttc aggacgctga ggttaccggt aaaatgacca tgccacatca
     1261 cggccttctg ccctgactgg ctgaaatcca gacggactgt ggcaggacgc gcccggcttg
     1321 tgagtactgc cccttcatca gccgcagcca ggcggttgaa ggccatgtca tttccgttga
     1381 tatcaagcgt gcctccccgg tatccccagc gaatgcggtc cccttcaatc tgatgactgt
     1441 cctgcagcac cacaactggt cgtccgctga caatactgac ttcactgaat gcccggaccc
     1501 tgcctgcagc atcagccttc tgtgccagaa tgaccgttcc gtcccccgta ttcagggcgc
     1561 cgggattttc accactgcca gccaccacca gtattccctt gccaatttta tgaagtgcgt
     1621 ctccagcaag tccgttcacc atccagtcaa cacggtgccc gtcg
//
LOCUS       DAZGAN020000293         1663 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGAN020000293 DAZGAN020000000
VERSION     DAZGAN020000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.295"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..660
                     /locus_tag="ACHKYO_RS26100"
                     /old_locus_tag="pgaptmp_005220"
     CDS             <1..660
                     /locus_tag="ACHKYO_RS26100"
                     /old_locus_tag="pgaptmp_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431854.1"
                     /translation="GSRIPSMQACLSRRSMVAMEGFVLYAGTNGLVSVDVNGNTALAT
                     EKIISPEQWQSQFNPASIVAYSWRGEYIACYTKPDGKQDVFVFSPVNMDIRYLSTPFD
                     CAWVDLAKDMMRVVTGDKMSVLAGSSLPSTIRWHSKIFSLPERTSFSCIRVKSPAPER
                     VGITIMADDVPVIHFAPGTFKGSVVRLPAATGQNWQVMVSGFGQVERITLSTSMSEMP
                     V"
     gene            657..>1663
                     /locus_tag="ACHKYO_RS26105"
                     /old_locus_tag="pgaptmp_005221"
     CDS             657..>1663
                     /locus_tag="ACHKYO_RS26105"
                     /old_locus_tag="pgaptmp_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4431855.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VS"
BASE COUNT          415 a          348 c          500 g          400 t
ORIGIN      
        1 ggctccagaa ttccttccat gcaggcatgc ctgagccgaa gaagtatggt ggcgatggag
       61 ggattcgtac tctatgctgg gacaaacggt ctggtatctg ttgatgtaaa cggtaataca
      121 gcactggcaa cggaaaagat tatttcacct gaacagtggc agagtcagtt taacccggcg
      181 tccattgtgg cttattcctg gcgtggtgag tacattgcct gttacacgaa accggatggt
      241 aagcaggatg tgtttgtatt cagtccggtg aacatggata tccgttatct cagtacaccg
      301 tttgactgcg catgggttga tctcgcgaaa gatatgatgc gcgtggtgac aggagacaaa
      361 atgtcagtgc ttgccgggag ctctctgccc tccacgataa ggtggcattc aaaaattttt
      421 tcattacctg aaagaacctc tttttcctgt atcagggtga aatctccggc gcctgagcgg
      481 gtggggatca ccattatggc tgatgatgtt cctgtgattc attttgcgcc gggtacgttt
      541 aagggaagtg tggtgagact tccggcagca accgggcaaa actggcaggt gatggtatcc
      601 ggattcgggc aggtggaacg aataaccctg agtacatcga tgtcggagat gccggtatga
      661 ccagaaaacc gtggcgtgcg gggaaggatt tatccacagt tgtggagaac atggaaattg
      721 gcaccgggca gcgtggtgac ggacgccacg catttgtgac ccgtgaggaa ctggttggtc
      781 ttaaactcgc ccggcgtcga acatcgggtg gtgcctcata tgcactgaat ccgggtattg
      841 agattgacag tactttaatg actgttgatt ttcccacaaa accgctgaat tttaaggcga
      901 caggaggatt tggctcggtt cttcttgaat gggatatgcc taattatcgc ggacattcac
      961 tgactgaaat ctggcggggt acggaggatg accttgctga tgcagtgctg gttgccacga
     1021 cgccggggca ggtttacggc gatccggttg accctggctg gtcgggattt tactggatac
     1081 gttttgttaa cgcggcagga gtgaaaggtc catggaatgc tgaaaaaggc actcaggcac
     1141 aaacacagat cggcgtgaag gccatcattg accagatccg cgatgaggct gcaaagtcgc
     1201 cggttgtgtc cgagctgcgt aaagaaataa aaaacgcgca ggggcaggct gtaaaggatg
     1261 ctgcaattaa gacaaccgaa gttgtgggga ctctcaggga agaaacgaca agaacgattg
     1321 gtggtattga aacccgcatt agcacactgg attcgtcaac cagtgaatcg cttaatgagg
     1381 tcgacaagcg catcactaaa ctggataaag aaggcggtga ggcgtttctg gcaatgtggt
     1441 caaaaaaagc gggagttgat ggtatcactg cggggatcgg gattgtcgcc ggaaaagaca
     1501 gtgaaggcag gcctgtaagt caggttgcaa tttctgcgtc gcagttgttt gtctttgacc
     1561 cgaacaaccc ggataacacc gcctatccgt ttgcggtatc aggtggcaag gttgtgatcc
     1621 cgaaagcgat gatttatgac gcggtgattg aaacactggt gtc
//
LOCUS       DAZGAN020000294         1661 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGAN020000294 DAZGAN020000000
VERSION     DAZGAN020000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.296"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1572
                     /locus_tag="ACHKYO_RS26110"
                     /old_locus_tag="pgaptmp_005222"
     CDS             <1..1572
                     /locus_tag="ACHKYO_RS26110"
                     /old_locus_tag="pgaptmp_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4431856.1"
                     /translation="QLARRSTVTPGGKACQYNDIILADHCLHDVQDMSGKNHPKADVA
                     KGQYGTVGQGLHIAKKLLPFIPADAGILLVPCCRGGSAFTAGADGTYSDSTGASEDSA
                     RWGVDKPLYKDLISRTKAALAKNPKNRLLAVVWMQGEFDIDAKPTEHSALFLAMVEKF
                     RADLAEQAEQCTGGSAAGVPWICGDTTYFWKQKNEPAYQAIYGGYKNKTDKNIHFVPL
                     MTDENGVNVPTNNPAEDPDIESIGYYGSTWRNSAATWTSADRASHFSAWARRGIISDR
                     LATAVLTHAGRTTVKADVPSSETEAVTTTLLSYRVSESEGNLKAQGWEPAGGKAEIIS
                     DAGGTGGKAMKLTKETGKSSWYLDHDAGTGAELLKNGGLISCRFKVPGDLVANQYVMA
                     LYWPVSSLPQGVTLTGDAGNNLLASFYIQTDAKDLNVMYHNAKVATNNQKLGTFGAFD
                     NEWHTLAFRFAGNNSLQVIPVIDGQDGAAFTLTQSPVGTFPVDKLRVTDITKNATYPV
                     LIDSIVVEVKKAVTE"
BASE COUNT          447 a          384 c          463 g          367 t
ORIGIN      
        1 cagttagccc gtcgcagtac agtcacgccg ggaggtaaag cctgtcagta taacgacatc
       61 attctggccg accactgtct gcatgatgtg caggatatga gtggaaaaaa tcacccgaaa
      121 gcggatgtgg ctaaagggca atatggcacc gtgggtcagg ggcttcacat tgccaaaaaa
      181 ctgctgccgt ttattccggc agatgcgggg attctgctgg ttccctgctg ccgtggtggt
      241 tctgcattca cggcgggtgc tgacggcacg tacagtgaca gcacgggtgc ttcagaggac
      301 tcagcccgct ggggtgtgga taagccgctg tataaggact taatcagccg gacaaaagcg
      361 gcactggcga agaacccgaa aaaccgcctg cttgctgtgg tgtggatgca gggcgagttt
      421 gatattgatg cgaagccgac ggagcattct gcgctgtttc tggcgatggt ggaaaaattc
      481 cgcgcagacc tggctgaaca ggcggaacag tgtaccggtg gcagtgctgc tggcgttccg
      541 tggatttgtg gcgataccac gtatttctgg aaacagaaaa atgaaccagc ataccaggca
      601 atctatggcg gctacaaaaa caaaacagat aaaaacattc atttcgttcc attaatgaca
      661 gatgaaaatg gcgtgaatgt acccaccaac aatccggcag aagacccgga tattgaatcc
      721 attggttatt acggctcaac gtggcgtaac agtgccgcta cctggacatc tgcggaccgg
      781 gccagccatt tcagtgcctg ggcgcgccgg ggtattattt ctgaccgtct ggccacggct
      841 gttctgactc atgcagggcg aaccacagta aaagccgatg ttccatcttc tgaaactgaa
      901 gcagtaacca caacactgct gtcttaccgt gtcagtgagt cagaaggaaa tctgaaagcc
      961 cagggctggg agccggctgg cggtaaagct gaaattatca gtgatgcagg aggcacaggt
     1021 ggtaaggcaa tgaaactgac caaggaaaca ggtaaatcat cctggtatct ggatcatgat
     1081 gccggtactg gtgctgaact gctgaaaaat ggtggtttaa tcagttgtcg ttttaaagta
     1141 cctggcgacc tggtggcaaa ccagtatgtc atggcgcttt actggccggt ttcctctctg
     1201 ccgcagggtg tcaccctgac aggggatgca gggaataatc tgctggcatc gttctacatc
     1261 cagacagacg caaaagacct gaatgtgatg taccacaatg caaaagtggc aacaaataac
     1321 cagaaactgg gaacctttgg tgcatttgat aatgaatggc atacgctggc cttccgtttt
     1381 gcaggaaata acagccttca ggttattccg gttattgatg gtcaggatgg tgccgcgttt
     1441 accctgacgc agtcaccagt aggaactttc ccggtggaca aactccgcgt aacggatatc
     1501 accaaaaatg caacttaccc ggtgctgatt gacagcattg tggtggaagt gaaaaaagcg
     1561 gtaacagaat aaaaaaatcc cgccggtcat gatatggctg gcgggagcta cctaacccat
     1621 aaaggaaaat aaaaatactc ccgaaggagt ggttatcagt c
//
LOCUS       DAZGAN020000295         1661 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGAN020000295 DAZGAN020000000
VERSION     DAZGAN020000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.297"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(113..1261)
                     /locus_tag="ACHKYO_RS26115"
                     /old_locus_tag="pgaptmp_005223"
     CDS             complement(113..1261)
                     /locus_tag="ACHKYO_RS26115"
                     /old_locus_tag="pgaptmp_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HGU4431857.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGALLFIIRASEY
                     VKALNDFSIWWLALTPPGGWFFLFCLRHWQWSNQMDEHLFFKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCYELPLQYGLVKKIDYLSDSGHKVEASLRVLLREITDK
                     FCQLPAALPVNVTLITDQPDSEIRSAFVSAWEALFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCLGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            complement(1265..1531)
                     /locus_tag="ACHKYO_RS26120"
                     /old_locus_tag="pgaptmp_005224"
     CDS             complement(1265..1531)
                     /locus_tag="ACHKYO_RS26120"
                     /old_locus_tag="pgaptmp_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HGU4431858.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            complement(1563..>1661)
                     /locus_tag="ACHKYO_RS26125"
                     /old_locus_tag="pgaptmp_005225"
     CDS             complement(1563..>1661)
                     /locus_tag="ACHKYO_RS26125"
                     /old_locus_tag="pgaptmp_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HGU4431859.1"
                     /translation="MCKGVTKSDFTYGYDEDIKEFIKDKTYPYGNW"
BASE COUNT          508 a          423 c          312 g          418 t
ORIGIN      
        1 gcgtgtttct gcccccagaa atgctccata tcgggttatc agccaccaga gcgcagccac
       61 accaataaat acaatccccg gtcgtccaaa gcgaccagac agacaggcca gtttattcat
      121 tctggtttcc tgatgtaaca gtactgataa aatgttcttc tccggatgag aataaagtca
      181 ggagagaatc attaccaaga cagaccagat cggctgccag agcagtaagc agccacggag
      241 cagcaggtcc cgggataccg caccattttt ccagtaacat acgtccggac ggttcccatc
      301 cggcaccata ctggtttcca atagtcatca gcggtgcagc aatcttttcc cagtgataac
      361 agtcccccag tacacgagcg gtacgacagg cagctgtctg ggtttcaagg aaaagtgtga
      421 attcatcatt aaacttgttg atatccagcg acatcgggcg tagcagacgg gctggatgcg
      481 gcaggttata tttttgcgca acatcatcgc tggtcaacaa aagtgcagcc agtccgtcag
      541 agtatgcatt tcccccatta agctgtatta ccagtatcaa atccacagtc agcactggtt
      601 gtttcagacg ctcatcaacc caccccatgg aaaagtcggg agtaacctcg atattgtcag
      661 gaacgacgcg ttggggaaat aacgcttccc atgcagaaac aaaagcagat ctgatttcag
      721 aatcaggctg atcggttatc agcgtcacat ttacagggag agcggcaggt aactgacaaa
      781 atttatccgt tatctccctt agcaagacac gtaaagaggc ttcaacctta tgcccactgt
      841 cagaaagata atctattttc tttacaaggc cgtactgcag aggcaattca taacaaagag
      901 tagctaccgt tattttatca ggcaaataaa cacaactggc agtaataaca agataccttt
      961 cggcccagga ttcccactgt ttctgagcat actccccctc ttttttaaaa aacaaatgtt
     1021 catccatctg gttgctccac tgccagtgtc gcaggcaaaa caggaaaaac cagccccccg
     1081 gaggagtcaa tgcaagccac cagatactga aatcatttaa tgctttcaca tattctgatg
     1141 cccggataat aaacaacaga gcaccaacaa cagccataaa tgcccctgtc ataaaccaca
     1201 acatcattga tggtttatga ggtaattctg ttgataatga ttttgttctg ctccatccca
     1261 tggtttatcc tattgcacat tccggtgcac tggatataac cttacaccca cacagacatt
     1321 tacatccatc aatcacaaca gctcttccat cagatatcca ttcaggagag ccctccacaa
     1381 taggattggt accatgtccg gaaacagggc aactaaccat atctccaaca agagctactt
     1441 ttttaccatc gacaatcatg gttgaagatg ccgaaataac tttaccacca tgtgtggtat
     1501 tatcaccaag caatacaaaa cctttagaca taaaactctc aaaaataaat tttcactttg
     1561 agtcaccagt taccgtaagg ataagtttta tctttgataa attccttaat atcttcatca
     1621 tatccatatg tgaaatcact tttagtaact cccttacaca t
//
LOCUS       DAZGAN020000296         1649 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGAN020000296 DAZGAN020000000
VERSION     DAZGAN020000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.298"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1649
                     /locus_tag="ACHKYO_RS26130"
                     /old_locus_tag="pgaptmp_005226"
     CDS             <1..>1649
                     /locus_tag="ACHKYO_RS26130"
                     /old_locus_tag="pgaptmp_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU4431860.1"
                     /translation="MSVIDAIGEGPVEGPVKGLQSILVNKTPLTDTDGNPVIHGVTAV
                     WRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTRTITSANIDRLRVTFGVQSLVETT
                     SKGDRNPSSVRLLIQLERNGHWVTEKDVTINGKTTSQYLTSVILNNLPERPFNIRMVR
                     ETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQLTVNYHIRGR
                     IIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKW
                     ALYAIGQYCDQTVPDGFGGTEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTL
                     TFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTE
                     LVEDPDAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRH
                     TPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDREVTLPEAGTSTVNLINGSGKPV
                     RVDITAHPAPDRIQVSALPDGVETYGVWGLSLPSLRRRLFR"
BASE COUNT          350 a          459 c          546 g          294 t
ORIGIN      
        1 atgagcgtga ttgatgccat tggtgaggga ccggtggaag gtccggtgaa gggactgcag
       61 agtattctgg tgaacaaaac cccgctgacg gacacggacg gtaatcccgt gatacacggt
      121 gtgaccgccg tctggcgtgc cggggagcag gagcagacac cgccggaagg ctttgagtcc
      181 tccggggcgg aaaccgcact gggcgtggag gtgacgaagg caaagccggt gacgcgcacc
      241 atcacgtcag cgaacattga ccgtctgcgg gtcaccttcg gggtgcagtc actggtggag
      301 accacctcaa agggtgaccg taatccctct tctgtccgcc tgctgattca gcttgagcgt
      361 aacgggcact gggtgacgga gaaggatgtc accattaacg gcaagaccac ctcgcagtac
      421 ctgacgtcgg tgattctgaa taatctccct gagcgcccct ttaacatccg gatggtcagg
      481 gagacggcgg acagcaccac ggaccagctg cagaacagaa cgctgtggtc gtcatacacc
      541 gaaatcatcg atgtgaaaca gtgctacccg aacacggcga ttgtggggct gcaggtggat
      601 gcggagcagt ttggtggcca gcagctgacg gtgaactacc atatccgtgg tcgcatcatt
      661 caggtgccgt caaactatga cccggaaaaa cgcacctaca gcggtatctg ggacgggagt
      721 ctgaaaccgg catacagcaa taacccggcc tggtgcctgt gggacatgct gacccacccg
      781 cgctacggga tgggaaaacg cctgggggcg gcggatgtgg acaagtgggc tctgtatgcc
      841 atcgggcagt actgcgacca gacggtcccg gatggtttcg ggggcacaga gccgcggatg
      901 acctttaatg cgtacctgtc acagcagcgt aaggtgtggg atgtcctggg ggatttctgc
      961 tcggcgatgc gctgtatgcc ggtatggaac ggccagacgc tgacgttcgt tcaggaccgt
     1021 ccgtcggatg tggtgtggcc gtacaccaac agcgatgtgg tggtggatga taacggcgtg
     1081 gggttccgct acagcttcag tgccctgaag gaccggcaca cggcggtgga ggtgaattac
     1141 accgacccgc agaacggctg gcagacctcc acggaactgg tggaagaccc tgacgccatc
     1201 ctgcgctacg gacgcaacct gctgaagatg gatgcgttcg gctgtaccag ccgcggtcag
     1261 gcccaccgtg ccggactgtg ggtgataaag acagaactgc tggaaacgca gacggtggat
     1321 ttcacgctcg ggtcacaggg gctgcggcac acgcccggtg acatcattga aatctgtgat
     1381 aacgactacg cagggaccct gaccggcgga cgcatcctgt ccatcgatgc cgccagccgt
     1441 accctgacgc tggaccgtga ggtgacactg ccggaagcag ggacatcgac ggtgaacctg
     1501 attaacggca gcggtaagcc ggtgcgcgtg gacatcactg cacaccccgc cccggaccgg
     1561 atacaggtca gcgccctgcc ggatggcgtg gagacatacg gtgtgtgggg actctccctg
     1621 ccgtcactgc gtcgtcgcct gttccgctg
//
LOCUS       DAZGAN020000297         1643 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGAN020000297 DAZGAN020000000
VERSION     DAZGAN020000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.299"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            468..>1643
                     /locus_tag="ACHKYO_RS26135"
                     /old_locus_tag="pgaptmp_005227"
     CDS             468..>1643
                     /locus_tag="ACHKYO_RS26135"
                     /old_locus_tag="pgaptmp_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HGU4431861.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDNSGTVVPAVAGGDIVWKYSQGSGE
                     GTLSQSGQVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTVRPE
                     "
BASE COUNT          394 a          312 c          518 g          419 t
ORIGIN      
        1 ataaacacga agacggtgaa cacctttctg tatatgggac ttggtaaaaa cggtcttaag
       61 tacaggagtg cgaagcacct ggttggacgt gcgtagccgg aatgcatatt cgtgagtcgg
      121 gtaatgagag ttgaacggtg gcagtgagaa tggaacagaa aaaaatcaga tgcggaaggc
      181 ggaaatacac agcgggtgcg atgtgcttca tgctgtttgt atggctgggt ggtgtggtga
      241 cattctgtgt gctggccgct ctgataatga ggtgaaaacg gaagagagtt tacttcctta
      301 attcgaactt tctgataact gagaacttgt taaatgctcg tgggtttata tatttaaatc
      361 aattggttaa tgctgacttc atctttatca gtcaaagttt gttttagtat gatgattgtg
      421 cccactgttt attgttgtcc gggagaaaat acagggaatt ccagggaatg aacaaaatat
      481 actcactcag agttaatgac agaaaagaac tggttgctgt accggaaacg gtggggggca
      541 ggaaaaagtc gtccggaatt cccggtgcgg ggatgctttc ccgtcttctt ctggcctcag
      601 gagccgttgc cggcgttctg ttttcttctc ctgcgctggc atccgttgtc gggaatactc
      661 tgccatggca gacctacagg gattttgctg aaaacaaagg ggcgtttcat gccggggcca
      721 caaatattcc gctttatggt aagaatgggg ctgtcagtgg gcggctggac aaggctccga
      781 tgatggattt cagtgttgtg gaccagatac tgggggtggc aacactgatt tctccgcagt
      841 atgtggcggg ggttaagcat aacggcagct acaatacggt gcgttttggt tatgcggacg
      901 atacgacata tcgccttgtt gaccgtaatg agcactggcg tgattttcat actccccggc
      961 tgaacaaact ggtgacagaa gttgccccgg tgagcgtcac ggatgccgga accggcaagg
     1021 gggtatacca gaaccgaagt cgttatccgg tgttttatcg tatggggagt ggaactcagt
     1081 acaccggagc tgcttccggt gcgctgacca gaatagcgga tgcttacgtc tggaggactg
     1141 gcgggacggt gggaagtccg gtcatttctg actggagtct ggtgtcaaac ccgggaaatc
     1201 tttaccaggg tgttaatggt ccgctggcgt cttacgggac atcaggagac agtggctcac
     1261 cgctttttgc ctgggatgcg gtgaagaagc agtgggtatt tgttgctgta cttcatggtt
     1321 atgccggtga gaacgggaag acgaactggt ttacggtgat tcctgcaggg gatgtgaata
     1381 acacgataaa gcaggacaat tcaggcacag ttgtgccggc agttgccggt ggtgacattg
     1441 tgtggaagta cagtcaggga agcggagagg gaaccctttc tcagagcggg caggtatgga
     1501 agatgaacgg cttcagggga gggagtctga atgacgggaa agatatcacc tttgggggta
     1561 agggcacggt tgttctgaag gatgatgttg tccagggggc tggttcgctc acgtttaatg
     1621 gtgattatac ggtcagaccg gaa
//
LOCUS       DAZGAN020000298         1635 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGAN020000298 DAZGAN020000000
VERSION     DAZGAN020000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.301"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            43..393
                     /gene="tnpB"
                     /locus_tag="ACHKYO_RS26140"
                     /old_locus_tag="pgaptmp_005228"
     CDS             43..393
                     /gene="tnpB"
                     /locus_tag="ACHKYO_RS26140"
                     /old_locus_tag="pgaptmp_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4431862.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            424..1435
                     /locus_tag="ACHKYO_RS26145"
                     /old_locus_tag="pgaptmp_005229"
                     /pseudo
     CDS             424..1435
                     /locus_tag="ACHKYO_RS26145"
                     /old_locus_tag="pgaptmp_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
     gene            1500..>1635
                     /locus_tag="ACHKYO_RS26150"
                     /old_locus_tag="pgaptmp_005230"
     CDS             1500..>1635
                     /locus_tag="ACHKYO_RS26150"
                     /old_locus_tag="pgaptmp_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4431863.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     Q"
BASE COUNT          410 a          434 c          448 g          343 t
ORIGIN      
        1 gctgctcaca gtgttgatcc gtgaactgac cgggagggga agatgatctc actcccatca
       61 ggtacccgta tctggctcgt tgccggcgtt accgatatgc gtaaatcctt caacggactg
      121 ggagaacagg tacaacatgt gctgaatgat aatcccttct ccggtcacct gtttatcttc
      181 cgtggccgac ggggtgacac cgtcaaaatt ctttgggctg atgctgatgg tctgtgcctg
      241 ttcaccaaac gcctggagga aggccagttt atctggcctg cggtacgtga cggcaaggta
      301 tccattaccc gctcgcaact ggcaatgctc ctcgataagc tggactggcg tcagccaaaa
      361 acatccagcc gtaactcact gacaatgttg taaaaaactc ctgaccgcat tataaaaacg
      421 gtcatgagtc agaaatacct cattcgcatc gcagagctgg aaaggttgct ctctgagcag
      481 gctgaagccc tccgtcagaa agaccagcaa ctgagtctgg ttgaagagac ggaagccttc
      541 ctgcgctctg cactgacacg tgccgaagaa aagatcgaag aagatgaacg ggaaatagaa
      601 catctgcggg ctcagataga aaaactgcgc cggatgctgt tcggtacccg ttctgaaaaa
      661 ctgcgtcgtg aagttgaact ggctgaggct ctgctgggac agcgatcgtt acagtgggcg
      721 ggaagacgat cctcaggttc cccgccagtt gcgacagtca cgccatcgtc gtccgttacc
      781 ggcacacctt ccccgtgaaa tacaccgcct ggagccagaa gaaagctgtt gcccggagtg
      841 tggcggtgag ctggattatc tgggggaagt cagcgctgaa cagctggaac tggtgagcag
      901 tgccctgaaa gtgatccgca cagaacgggt aaaaaaagcc tgtacaaaat gtgactgtat
      961 tgttgaagca ccggcgccgt cccgcccgat agagcgtggt atcgcgggcc ccggattact
     1021 tgcccgcgtg ttaacgggaa aatactgcga acatctgcca ctgtatcgtc agagtgaaat
     1081 ctttgcccgc cagggtgtcg aactgagccg ggccttactc tccaactggg ttgacgcgtg
     1141 ctgccagtta atgacaccgg tgaatgatgc cctgtaccgt tatgtaatga acacccgcaa
     1201 ggttcacact gatgacacac cggtaaaggt actggcaccg ggtcagaaaa aggcgaaaac
     1261 agggcgtatc tggacgtatg tccgggatga tcgcaatgtg ggttcgtcat ctcctccagc
     1321 ggtctggttc gcgtactcgc cgaaccggca ggggaaacac ccggagcaac acctccgccc
     1381 cttccggggt atcctgcagg cggatgcgtt cacaggttac gacaggttgt tcagtgatct
     1441 tacccagcaa tagtggacac gcggctaagt gagtaaactc tcagtcagag gtgactcaca
     1501 tgacaaaaac agtatcaacc agtaaaaaac cccgtaaaca gcattcgcct gaatttcgca
     1561 gtgaagccct gaagcttgct gaacgcatcg gtgttactgc cgcagcccgt gaactcagcc
     1621 tgtatgaatc acaac
//
LOCUS       DAZGAN020000299         1603 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGAN020000299 DAZGAN020000000
VERSION     DAZGAN020000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.302"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(299..451)
                     /locus_tag="ACHKYO_RS26155"
                     /old_locus_tag="pgaptmp_005231"
     CDS             complement(299..451)
                     /locus_tag="ACHKYO_RS26155"
                     /old_locus_tag="pgaptmp_005231"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431864.1"
                     /translation="MFEGLSDDSSPKEQQRDHRQHARGDTARRKSMTERSERIDEEAE
                     ENRKPH"
     gene            complement(896..1402)
                     /locus_tag="ACHKYO_RS26160"
                     /old_locus_tag="pgaptmp_005232"
     CDS             complement(896..1402)
                     /locus_tag="ACHKYO_RS26160"
                     /old_locus_tag="pgaptmp_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4431865.1"
                     /translation="MAAQEVITDAQIRQRLLDLEEQNKKLRQELLDERRNTNFTQTYP
                     KGWERIRNLIQANPGAARLYSVLSEHIDGNCGAVVADQQFLADQLSVTTRTIRNWVNF
                     LEENKCLVRIPIAGKLCAYALNPTEVWKGYNTSKPYAAFLTKTLVNSEGVIKRRLQAC
                     FGTSEPNE"
BASE COUNT          369 a          408 c          389 g          437 t
ORIGIN      
        1 aaaaaaacaa gcccttcagg gcgcgttagg gggtgtcggg gcgcagccct gacccagtca
       61 cgtagcgata gcggagtgta tactggttta ctcctctgca agcctcgtca gccgtgattt
      121 tgcccgattt cgcgctctcc acgttcaaca gctcgatcat gtttttgccg tccgttcacc
      181 gttaaaacgc ctcagaaatc gggacaggat gtgggaatgt cggaccgcgc acgcgcactc
      241 agacattccc cacctggtca gggcttcacc ccgacacccc gcatccgtgc gtaagtgctc
      301 aatgtggctt ccggttctct tccgcttcct cgtcgattcg ctcgctacgc tcggtcatcg
      361 acttgcggcg agcggtgtca cctctcgcat gttgtcggtg atcccgttgt tgttctttcg
      421 gggatgagtc gtcagatagc ccttcaaaca cgctcagaag ctctcagacg cacgaaacgg
      481 gttttagatg cccgccaggg catggaaggc gcgaaaaacg cttacagagg gtttcaggag
      541 gtcaggcgcg ccgatacccc taaccagggg ctttcaggtc gtcaggaaca gcgtcagccc
      601 cctgcaaagc ccatttgcac ggtacaggca gctattcggt tggtaggtgg tcgtagaccg
      661 tcggtatcac agcgataggg ggtctggggt tctccccaga cggtttccgg ttgcgctcaa
      721 ggcgcagccg aaaccgtggc ggctcggtcg ttctggagat cggggagcaa aaacacgcaa
      781 gggattttga ggcggcaaaa gagcaatgca ggatagacaa cccgatgtca aatgttaaca
      841 tttgaatcag attgtccaaa aacggggttt ttgcctgtgc ctgccgcagg ctctgttatt
      901 cattcggttc tgatgtgcca aaacatgctt gaagtcgacg ctttataacc ccctcgctgt
      961 taacaagtgt ttttgttaaa aacgcagcgt aaggtttgga tgtgttatag cccttccaaa
     1021 cctcagtagg attcaaagca taggcgcata attttcccgc gataggaatc ctaaccaagc
     1081 atttgttttc ctctaagaaa ttaacccaat tacgtatcgt tcttgttgtc acagataact
     1141 ggtctgctaa aaattgttgg tctgcaacga ctgcaccgca attaccgtca atgtgttctg
     1201 acaggaccga atacaatctt gctgctccag ggttagcttg tatgaggttt ctaattcgct
     1261 cccatccctt agggtaggtt tgagtgaaat tggtgtttct gcgctcatcc agaagctctt
     1321 gtcgcaattt tttattttgc tcttcgagat ccagaagtcg ttgtctaatt tgagcatcag
     1381 tgattacttc ttgagcagcc ataatacctc ccaattaatt tcctcataag cggaagaatg
     1441 attgcaaatt tatgttgttt tatttccaac gtcaatctat tcatttccta ttatgggaat
     1501 taaccggaaa gagcttgttt ccggttaacc ggaacgatcg tttccggtta cttttcgaaa
     1561 acccccataa aatcaaccat atagaacttt tctcctctta tct
//
LOCUS       DAZGAN020000300         1575 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGAN020000300 DAZGAN020000000
VERSION     DAZGAN020000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.303"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            241..420
                     /locus_tag="ACHKYO_RS26165"
                     /old_locus_tag="pgaptmp_005233"
     CDS             241..420
                     /locus_tag="ACHKYO_RS26165"
                     /old_locus_tag="pgaptmp_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4431866.1"
                     /translation="MTFLNQLMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            461..706
                     /locus_tag="ACHKYO_RS26170"
                     /old_locus_tag="pgaptmp_005234"
     CDS             461..706
                     /locus_tag="ACHKYO_RS26170"
                     /old_locus_tag="pgaptmp_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4431867.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEARLDAEVDA
                     ILDELLGAPAVPEPEGIAGEGSASYGGDPTPDSDMMM"
     gene            783..998
                     /locus_tag="ACHKYO_RS26175"
                     /old_locus_tag="pgaptmp_005235"
     CDS             783..998
                     /locus_tag="ACHKYO_RS26175"
                     /old_locus_tag="pgaptmp_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4431868.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            1002..1337
                     /locus_tag="ACHKYO_RS26180"
                     /old_locus_tag="pgaptmp_005236"
                     /pseudo
     CDS             1002..1337
                     /locus_tag="ACHKYO_RS26180"
                     /old_locus_tag="pgaptmp_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
BASE COUNT          437 a          328 c          454 g          356 t
ORIGIN      
        1 caactgacag gcatatcaaa ggcggcgaca tatacgctgc tgattgacag tgtgaaggtg
       61 gaagtgaaca acgcggacgt cgcggcatga taaaaaaggc cgccagcaat gaaggaaagg
      121 gatgaactgt gtccactaca tgaatgctgg cggctggtaa aacgaccatg atgacaccga
      181 agtctgcggt gtttttctac aaaaataagt aaatcatgtc aactatcacg gtgagaaacc
      241 atgacatttc tgaaccagtt aatgctgtac ttctgtacgg cggtctgtgt gctgtatctt
      301 ctttcgggtg ggtacagggc agtgcgcgat ttctggcgca ggcagattga taaaagggcc
      361 gcagagaaaa tcagcgccag tcagtcagcc ggaagcaaac ccgaagagcc gctcatttag
      421 ctgcaacttt cttaatcaca cctttcgacg agaaaatccc atgtcagaaa ttacatccct
      481 ggtcactgca gaggcagtga aggaagtcct gcgctctgaa gaagtcctga gcgcactgaa
      541 acagaaactc cgccagaacc ttgaggcgcg tcttgatgca gaagtggatg ccattctgga
      601 tgaactgctg ggcgcaccgg cagttccgga gccggaaggt atcgcgggtg aggggagtgc
      661 ttcatatggc ggtgacccca cacctgacag cgacatgatg atgtaagcat gcgcaaggga
      721 ccatcggtgt gtgccggtgg tctttatatt gttgtgagct tccggattgc gggagacggg
      781 gtatgtacca gatggaaaaa atcacaacag gtgtgtcata caccacgtca gcggtgggaa
      841 cgggctactg gttcctgcag ttgctggaca gggtttcccc gtctcagtgg gcggcaatag
      901 gtgtgctggg gagtctgctg tttggtctgc tgacatatct gacgaacctg tatttcaaaa
      961 tcagagagga ccggcgtaag gcggcgcggg gagagtagac gatgaataaa caatacgaac
     1021 tggttgttaa agggataaat aattacccgg ataagattac tgttactgtg gcgctggaaa
     1081 ctggtgggta tacgtcactg ttgttgccaa atgtggtgat tgatcttgat cgtgttgaag
     1141 gtgccccgct ggagttttac gaagctgagg cgaaaaagaa ggcgaagcag tttttcatgg
     1201 atattgctgc cgggttatgt gaagggaacg aaccgttgcc ggaaaagcgc cccataattt
     1261 tagaggcgca aaatgtgttg ataacctaca aaggaaagct accgggaaga attactgatt
     1321 ctctgaagat gccgccgtca acattgcggt cagaaaaaga tgatgttgaa tcacgtattg
     1381 aaaaactgga gcgctatatc gctgaattga aaaaaagcac cccaacaaaa aatgaggtgc
     1441 ttgcagcaga tgaaatgaaa gaagctattc ttgatcgcgc ggcgcatcta agctgcgctt
     1501 cacggttgaa agagcatctt cagcagcctg aaaaaaacgc cgcgatgagc agtttgcggc
     1561 gttttacgat tattg
//
LOCUS       DAZGAN020000301         1548 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGAN020000301 DAZGAN020000000
VERSION     DAZGAN020000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.304"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(348..506)
                     /locus_tag="ACHKYO_RS26185"
                     /old_locus_tag="pgaptmp_005237"
     CDS             complement(348..506)
                     /locus_tag="ACHKYO_RS26185"
                     /old_locus_tag="pgaptmp_005237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431869.1"
                     /translation="MSAFEGLSGDSSPKEQQRDHRQHARGDPARRKSMTERSERIDEE
                     AEENRKPH"
     gene            complement(583..873)
                     /locus_tag="ACHKYO_RS26190"
                     /old_locus_tag="pgaptmp_005238"
     CDS             complement(583..873)
                     /locus_tag="ACHKYO_RS26190"
                     /old_locus_tag="pgaptmp_005238"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431870.1"
                     /translation="MIVYPALLFCRLKILCVFLLPVLQKKPSRHGSGSALSATGNRLG
                     RTPDPHRCNTDGLRQPTNRMAACSVQMGFAGGWRGSWRPESPWLGVSARLTS"
     gene            complement(921..1397)
                     /locus_tag="ACHKYO_RS26195"
                     /old_locus_tag="pgaptmp_005239"
     CDS             complement(921..1397)
                     /locus_tag="ACHKYO_RS26195"
                     /old_locus_tag="pgaptmp_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4431871.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          355 a          411 c          385 g          397 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacc tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca cgcacgccga tacgattacc
      301 acctggtcag ggcttcaccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccgttgtt gttctttcgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcacaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggttgt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttagcac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccaac gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cgcgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat ttttccctct gaaaatccca tgcactaacg ttggttcaaa
     1441 tctaagccat catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca tccaggatgc
     1501 gacaacgttg tgtcaccctg gatgcgacag gtaaataaaa aacacact
//
LOCUS       DAZGAN020000302         1513 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGAN020000302 DAZGAN020000000
VERSION     DAZGAN020000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.305"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1513
                     /locus_tag="ACHKYO_RS26200"
                     /old_locus_tag="pgaptmp_005240"
     CDS             <1..>1513
                     /locus_tag="ACHKYO_RS26200"
                     /old_locus_tag="pgaptmp_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HGU4431872.1"
                     /translation="LDERIVAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERL
                     LLLAPGKRQEIKAEDINWLPEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDI
                     MPSYDTIRRAMKKLPEVVKQKGRVTGSEYRQLEGFTRRDWSKMPVNYVWIGDGHGMKL
                     KCRHPVHGRPFAPEVTFVIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYY
                     SDNGSGETADILDKEVVGILPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRT
                     YFGKGADRETLRKTNRDLRSAFSALQQGKRLNARQQSAMRDLPSWAELIDAIRDGVEW
                     YNNRPHDELPIKPNGKHYSPAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRC
                     EIRWLNNIYYAPELRDEHGRKVLISYDIHDAERITVRRLDGSVICEAVWGGNKREAFP
                     VSAEYYKQQQRLKGMRKRAEEKIRDAEDEVVNVLEHKQQEPWLENIYRPVGNAV"
BASE COUNT          387 a          376 c          452 g          298 t
ORIGIN      
        1 ctggacgagc gcattgtggc aatggttgag acggcgaacg ccaaaaaggg aaacagccgt
       61 gcgttgagtg aaatcacgct gaaacgctgg attgcagcct ttaacaaggc gcagaacgcc
      121 gccgaacgcc tgcttttgct ggcaccgggt aagcgccagg aaataaaagc cgaagatatt
      181 aactggctgc ccgaatttct ggcgcagtat cgccagtcaa acggccgacc aatgaccgag
      241 gcctacgagg attttgtcgc tgaatggcag caccggcacg ctgatgagcc ttatatgctc
      301 gatatcatgc cgtcttatga caccattcgc cgcgcaatga agaaactgcc ggaagtggtg
      361 aaacaaaaag gccgggtgac cggcagtgaa tatcgccagc ttgagggatt cacgcgccgc
      421 gactggtcaa aaatgccggt gaattatgtc tggattggtg acggtcacgg catgaagctg
      481 aaatgcaggc acccggttca cgggcggcca tttgcaccgg aagtgacctt tgttatcgac
      541 ggtggcacgc gctttgtggt gggctggagc cttgacctgg ctgaaaatgt tttcgccgta
      601 gccggtgcca tacagcacgg tattcgtaat cacggcaaac cgtttctgta ttactcggat
      661 aacggttccg gggaaaccgc cgacatcctg gataaggaag ttgtggggat tctgccgcga
      721 ctggggatta atcacccgac agggattgcc ggtaacccgc agggacgggg cattatcgaa
      781 cggcttaacc gcacattacc gatgcgcata gcccgtaaat accgcaccta tttcgggaaa
      841 ggtgcagatc gcgagacgtt acgtaaaacc aaccgcgatt tacgctcggc attcagtgcc
      901 ctgcaacagg gcaaacggct gaacgcccgg cagcagtcag cgatgcgtga tttaccgtcc
      961 tgggctgaac tgattgatgc cattcgtgat ggtgttgagt ggtacaacaa ccggccgcac
     1021 gatgaattac ccataaaacc gaacggcaag cattacagcc cggcggagtt cagaaaaaaa
     1081 cgcctggcgg aagaggacac ggaaattgaa tggctgtccg atgttgagct gcgggatatg
     1141 ttccggccga tggtggaacg ccctgtaaga cgctgtgaaa tacgctggct gaataatatt
     1201 tactacgctc ccgagctgcg tgatgagcat ggccgcaagg tgcttatcag ctatgacatt
     1261 catgatgccg aacgaattac cgtacgtcgc ctggatggca gcgtgatttg cgaggcggta
     1321 tggggcggca ataaacgcga agccttcccg gtcagcgcgg aatactacaa acagcagcaa
     1381 cgccttaaag gcatgcgtaa acgcgcagag gaaaaaatcc gtgatgccga ggatgaggtg
     1441 gtcaacgtgc tggagcacaa gcagcaggag ccatggctgg aaaacatcta tcgccctgtg
     1501 ggtaatgcgg tga
//
LOCUS       DAZGAN020000303         1506 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGAN020000303 DAZGAN020000000
VERSION     DAZGAN020000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.306"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(112..765)
                     /gene="ribB"
                     /locus_tag="ACHKYO_RS26205"
                     /old_locus_tag="pgaptmp_005241"
     CDS             complement(112..765)
                     /gene="ribB"
                     /locus_tag="ACHKYO_RS26205"
                     /old_locus_tag="pgaptmp_005241"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HGU4431873.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(869..1017)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1139..1429
                     /gene="ubiK"
                     /locus_tag="ACHKYO_RS26210"
                     /old_locus_tag="pgaptmp_005242"
     CDS             1139..1429
                     /gene="ubiK"
                     /locus_tag="ACHKYO_RS26210"
                     /old_locus_tag="pgaptmp_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HGU4431874.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT          417 a          394 c          343 g          352 t
ORIGIN      
        1 ttctcataga aagagaagcg gaattaacat cttgcatagc ggggcaaaat aaaaaccgaa
       61 gccagctaca acttcggttt cttgattaag gcagtaatta agcagcggtt ttcagctggc
      121 tttacgctca tgtgcctgac ggtatgccac caggtcttca atagtcacga gcgccatatt
      181 gtgtttattg gcaaactcaa tgcactctgg tgcacgcgcc atcgtgccat cgtcattagt
      241 cagctcacac agcacaccag ccggtttaaa gcctgccagc gtcatcaaat caatagtcgc
      301 ttcagtatga ccgccacgcg tcagtacacc acctgcctgc gcgcgaagtg ggaaaacgtg
      361 gcccgggcga ttcagatcag aaggttttgc gccatcggca atcgctgcgc gaacggtcgt
      421 aatacgatca gcggcagaaa caccggtagt cacaccttca gccgcttcaa tggtcacggt
      481 aaaaccggtg ccataggcgc tggtgttatt ttctaccatc attggcagat caagttgttt
      541 acggcgatct tcagtgatgc acaggcaaac aataccgcta ccgtggcgaa tggtcagcgc
      601 catctgctca acggtcatgg tttctgccgg gaagatcata tcgccttcgt tttcacggtc
      661 ttcatcatca agcaccatta caccgcgtcc ttcacgcagc gcagccagtg cattttcaac
      721 acgttcgaaa ggcgtaccaa aagaggaaag tagcgtctga ttcatggtaa aaaaacctca
      781 ctaaaattat ggttaccaga atcagggcag tcttaggagt ggcggcatat agccaaaata
      841 acgtgagcgg gtccatgccc gacagaatcg ttactctctc ccatccggac tctaaccgtc
      901 ggccccggaa ttacaccgga tctgctgtcc tttgagttcg cacccaaagc gctcgcgggc
      961 tttcaactga gttgatttac cgccggtggg gaatttcgcc ccgccctgag aataagcgga
     1021 ttcactataa cgctaatgat tagcggcagc aacgcatagc ttcacataat tctggtttat
     1081 gacttaccct tatcgcacta caatggtact caacacctat cagtacaggg aaactacaat
     1141 gattgacccg aaaaaaattg agcaaatcgc tcgccaggtt cacgaatcaa tgcctaaagg
     1201 aatcagggag ttcggggaag atgtggagaa aaaaatccgc caaaccctac aagctcagct
     1261 gacgcgcctc gaccttgtaa gccgtgaaga gttcgacgtc caaacgcaag tcctgttacg
     1321 gactcgtgag aaactggcgc tgcttgaaca aagaatgagc gaactggaaa atcgcagtac
     1381 tgagattaaa aagcagccag atccagagac tctcccccca acgctgtaaa catttgtctt
     1441 taatgcactg taaattatga attaatcctc taatttacag tgctttctat taccttatcg
     1501 cttaat
//
LOCUS       DAZGAN020000304         1505 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGAN020000304 DAZGAN020000000
VERSION     DAZGAN020000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.307"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..225
                     /gene="ydcD"
                     /locus_tag="ACHKYO_RS26215"
                     /old_locus_tag="pgaptmp_005243"
     CDS             <1..225
                     /gene="ydcD"
                     /locus_tag="ACHKYO_RS26215"
                     /old_locus_tag="pgaptmp_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdcD"
                     /protein_id="HGU4431875.1"
                     /translation="INLNFFNDILYSVRLKNISKLENMEFCAKKQRVYFSDKNKKASY
                     KIINYGDYYDVDYYDNNLKNEVFDWIGKWS"
     gene            complement(140..325)
                     /locus_tag="ACHKYO_RS26220"
                     /old_locus_tag="pgaptmp_005244"
     CDS             complement(140..325)
                     /locus_tag="ACHKYO_RS26220"
                     /old_locus_tag="pgaptmp_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431876.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSQKL
                     HFSNCYHNNQHRNSLHN"
     gene            407..1505
                     /locus_tag="ACHKYO_RS26225"
                     /old_locus_tag="pgaptmp_005245"
                     /pseudo
     CDS             407..1505
                     /locus_tag="ACHKYO_RS26225"
                     /old_locus_tag="pgaptmp_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          558 a          197 c          346 g          404 t
ORIGIN      
        1 ataaatctta attttttcaa tgatattctt tatagcgtgc gattaaaaaa cattagtaaa
       61 ctggagaata tggagttctg cgctaaaaaa caacgggtgt attttagtga taaaaacaag
      121 aaggctagtt ataaaataat taattatgga gactattacg atgttgatta ttatgataac
      181 aatttgaaaa atgaagtttt tgactggatt ggtaaatggt cataatgaac aacaccagaa
      241 agagaaaaat ataaaataca tacagaaaga catgagagca agggacgggg cagggcaaga
      301 tcatgaaaat tgtgatgtaa atcacgattt tcatcttggc tttaacgcct acaggtgatc
      361 aaaatttaac ctttggaata actaaaaaga taaaaaagga cgccaggtga gtattcaaag
      421 tttgcttgat tatatttcag tgatccctga tatacgacaa caaggaaagg ttaaacataa
      481 attatctgct attttgtttc tcaccgtatg tgcagtaatt gcaggtgccg atgagtggca
      541 ggaaattgaa gattttggac atgaaagact tgaatggcta aagaaatatg gtgattttga
      601 taatggcatt ccggtcgatg acaccattgc acgcgttgtg agtaacattg acagtttggc
      661 ctttgaaaag atgtttattg aatggatgca ggagtgccat gaaatcactg atggtgaaat
      721 tatagcaata gatggaaaga ccataagagg ctcctttgat aagggaaaaa gaaaaggagc
      781 aatccatatg gtgagtgcat tctcgaacga aaatggtgtt gtactggggc aggtgaaaac
      841 ggaagccaaa agtaatgaga ttacagccat tccagagttg cttaacctac tgtatttaaa
      901 gaaaaatttg ataaccattg atgctatggg ctgtcagaaa gatatcgctt cgaagatcaa
      961 agataaaaaa gcagattatc ttctggcagt aaaaggcaat caggggaaat tacatcatgc
     1021 attcgaggaa aaatttcctg taaatgtgtt ttctaattat aaaggcgatt cgtttagtac
     1081 gcaggagata agtcatggaa gaaaagaaac acgtttgcat attgtcagta acgtaacgcc
     1141 tgaacttttg tgattttgaa ttcgaatgga agggattaaa aaagctttgt gtagcattgt
     1201 cattcaggca gaagaaagaa gataaatcag cagaaggtgt aagcatccga tattatattt
     1261 catcaaagga tatggatgct aaagaatttg catatgctat cagagcgcac tggctgatcg
     1321 agcacagtct tcattgggtg ttagatgtaa aaatgaatga agatgccagc cggataagaa
     1381 gaggaaacgc agcctaaata atatctggaa taaagaagat ggcactgaat ttattaaggg
     1441 attgcaaaga cattaagggt gaagaggaaa agaaagaagg ctgcgttaaa cacagggaaa
     1501 gatca
//
LOCUS       DAZGAN020000305         1486 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGAN020000305 DAZGAN020000000
VERSION     DAZGAN020000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.308"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..320
                     /locus_tag="ACHKYO_RS26230"
                     /old_locus_tag="pgaptmp_005246"
     CDS             <1..320
                     /locus_tag="ACHKYO_RS26230"
                     /old_locus_tag="pgaptmp_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431877.1"
                     /translation="DNEGRIIESCLIRYFDPKLQDDDKESKRSDLNKMFMDLVENKKI
                     KKIYVNFQYERNNEYFCFYSKVVPPKFHHFFVLDFSDGKINIDRDQDLMTQMALCMGY
                     YNY"
     gene            complement(759..1295)
                     /gene="yfdR"
                     /locus_tag="ACHKYO_RS26235"
                     /old_locus_tag="pgaptmp_005247"
     CDS             complement(759..1295)
                     /gene="yfdR"
                     /locus_tag="ACHKYO_RS26235"
                     /old_locus_tag="pgaptmp_005247"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4431878.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVMPLAPGHA
                     YGMFMERFNELSELRTYA"
     gene            complement(1424..>1486)
                     /locus_tag="ACHKYO_RS26240"
                     /old_locus_tag="pgaptmp_005248"
     CDS             complement(1424..>1486)
                     /locus_tag="ACHKYO_RS26240"
                     /old_locus_tag="pgaptmp_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431879.1"
                     /translation="LLVEKFKDSKVETFIGTFTA"
BASE COUNT          416 a          294 c          318 g          458 t
ORIGIN      
        1 ttgataatga gggaagaatt attgaaagct gcttaatacg ttactttgat cctaagcttc
       61 aagatgatga caaggaaagt aaaaggtcag atttaaataa aatgtttatg gacttggtgg
      121 aaaataaaaa aattaaaaaa atatacgtta attttcaata tgaaaggaat aatgagtatt
      181 tctgctttta ctctaaagtt gttccgccga aatttcatca tttctttgtg ttggattttt
      241 ctgatggcaa gataaatatt gatagagacc aggatctgat gactcagatg gcattgtgca
      301 tggggtatta taattattaa tatttcgcct gataccaact caaaatcttt tttgtcgcat
      361 ccattgtgga ttaatattca gaggtgagga gtgtctgcgg aatttatatt ttgcaggatg
      421 agcatacaat acatgatata acatctgttt ttggcacaga gcatatagtc agattatgtt
      481 tagttctgtg ccatagatgg atactatctc acctcttggc taactctcat tactcaataa
      541 ctccagcaaa tctgtatatt ttgcgtgatg cccatttatt tgggcaggat ttaatatcag
      601 gatctggaaa atctggcctg tatttctggc ctgtcctcct gtttacgctg ttccagcgaa
      661 gcaccgtcga tattgaaacg ccacagaatt cggcgacttg ttttgttgtc attaagttgc
      721 tcattgctac ccctccagcg gttgttcagt tgaaagactc atgcgtatgt gcgtaactcc
      781 gataactcgt taaagcgttc cataaacatc ccgtaggcat ggcccggtgc cagtggcatc
      841 acgttgaaca tctctgttgc cgggatacct tccagtactg gccagaaaga gccatcatca
      901 agcccgagat cgcggcgttc ggttgccagc atgatgagat cggcatattt cacaggcgtg
      961 ctcataaccg ggggtaaccc gtatttctca cggattacgg cgtctatttt ttcttccatc
     1021 cgtttatagt caggaagaag gcgtttcagt ggagcgggaa tatcctggca atacgcttct
     1081 gttgcatcat gcattaacgc ttcaaaagca aattcctgcg gcaccagctg gctgcaaagc
     1141 accgcatgct gggcgacact gtagaagtgt gaaagatgtc ctgcaaagcg acagatattt
     1201 gaaagggaaa ctgcgatatc gttaatcacg atgtcgtctt tatttatcct gtcataataa
     1261 aaatgcttcc cggaaaaagt tttaataaat gacatttcgt tctccacttt atatgcgctg
     1321 caccgcgttg aattctgcta aaaggaagct ctcaccatcc ggtgattatt gagttaatta
     1381 cgtttccata aatgcccccg caggggcatt tgcagtaatg aaatcaggcg gtgaaagtac
     1441 caataaaggt ttctactttg ctgtctttga atttctcaac aagcag
//
LOCUS       DAZGAN020000306         1464 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGAN020000306 DAZGAN020000000
VERSION     DAZGAN020000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.309"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            26..1204
                     /gene="tssD"
                     /locus_tag="ACHKYO_RS26245"
                     /old_locus_tag="pgaptmp_005249"
     CDS             26..1204
                     /gene="tssD"
                     /locus_tag="ACHKYO_RS26245"
                     /old_locus_tag="pgaptmp_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HGU4431880.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLAKTSETSGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSIYPPMKNIMDHTK
                     "
     gene            1201..>1464
                     /gene="yrhA"
                     /locus_tag="ACHKYO_RS26250"
                     /old_locus_tag="pgaptmp_005250"
     CDS             1201..>1464
                     /gene="yrhA"
                     /locus_tag="ACHKYO_RS26250"
                     /old_locus_tag="pgaptmp_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhA"
                     /protein_id="HGU4431881.1"
                     /translation="MMTKTQINKLIKMMNDLDYPFEAPLKESFIESIIQIEFNSNSTN
                     CLEKLCNEVSILFKNQPDYLTFLRAMDGFEVNGLRLFSLSIPEP"
BASE COUNT          546 a          240 c          246 g          432 t
ORIGIN      
        1 catgccataa aaggactttt cagggatgag taatattgtt tacctgacag taacgggaga
       61 acaacaagga agcatctccg caggttgtgg gacttctgag tctacaggta atcgttggca
      121 gagcgggcat gaggatgaaa tatttacatt ctcactctta aataatatta ataatacggg
      181 gcttggttca cagttccatg gtataacatt ttgtaaatta attgataaaa gcactccatt
      241 atttattaat tccattaaca ataatgaaca attatttatg ggatttgact tctatcgaat
      301 aaatagattt ggtagattgg aaaagtatta ttatatacaa ctaagaggcg cttttttatc
      361 ggctattcat caccagatca ttgaaaacca actggataca gaaacaataa ctattagtta
      421 tgaatttatc ctctgtcaac atcttatcgc aaataccgag ttcagctatt tggcactccc
      481 tgaaaattat aaccgtttgt ttttaccaaa ttcaaaaaac caaacaaata atcgtttcaa
      541 aacgttaaac agcaaagcta ttggcaggct acttgctgct ggtggcgtat acaatgggaa
      601 cattgaagga ttcagagata ctgcggaaaa actgggtgga gatgcaataa aaggctatga
      661 tcaaatacta aatgaaaaaa cagcgggcat agcgatagca acagcatcta ttcttttaac
      721 aaagcgttct aatgttgata catatacaga aataaatagt tacttaggca aacttagagg
      781 tcaacaaaaa cttcttgatg gtatagacat aatagaaata atatacatta agagaccttc
      841 aaaagactta gctaacttac gaaaggagtt taataaaact gtaagaaaaa attttcttat
      901 caaacttgca aaaacctccg aaacatctgg aagattcaac gccgaagacc ttttaagaat
      961 gagaaagggc aatgttcctc taaattataa tgttcaccat aaactatctc tagatgatgg
     1021 tggtactaat gatttcgaaa atttagtatt aatcgaaaac gaaccatatc ataaagtttt
     1081 tactaacatg caatcacgaa tagctaaggg aatattagta ggtgaaagca aaatcactcc
     1141 ctgggccatt ccatctggct caatttatcc tcccatgaaa aatattatgg accacacaaa
     1201 atgatgacta aaacccaaat aaataaatta ataaaaatga tgaatgattt agactatcca
     1261 tttgaagcac cgctcaagga atcatttatt gaaagtataa tccaaataga atttaattct
     1321 aattcaacta attgcctgga gaagttatgt aatgaagtta gtattctttt taagaatcaa
     1381 cctgattatc ttactttttt aagagcaatg gatggattcg aagttaatgg attacgatta
     1441 tttagcctct cgattccaga acct
//
LOCUS       DAZGAN020000307         1423 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGAN020000307 DAZGAN020000000
VERSION     DAZGAN020000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.310"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..212
                     /locus_tag="ACHKYO_RS26255"
                     /old_locus_tag="pgaptmp_005251"
     CDS             <1..212
                     /locus_tag="ACHKYO_RS26255"
                     /old_locus_tag="pgaptmp_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HGU4431882.1"
                     /translation="KDKAAGVSAETAQQRIKEFHSEKFFHSLRSTTTFAGRKYTNSDM
                     PSPKKMKLMADTISAVYLDGYEGRQ"
     gene            610..>1423
                     /locus_tag="ACHKYO_RS26260"
                     /old_locus_tag="pgaptmp_005252"
     CDS             610..>1423
                     /locus_tag="ACHKYO_RS26260"
                     /old_locus_tag="pgaptmp_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014228715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4431883.1"
                     /translation="MTTLYIYLVLAASLSALSTDMIVPALPELQKAFHADYSLIQLSI
                     SGFLIVFAFSQLISGTIGDYFGKVRVMTICLIIFLIGSIVCFMANDLNMLLLGRILQA
                     IGAGAGPVISKAIAKESFPPLKLKRALSDISSTSAIIPLIAPLAGALILDYYNWNVIF
                     IVMGAFSILTILLSPKETNKQEKKTHQDNVKFVTKDFICGTILVSLMLSSLFCYISIS
                     PALFMSDYGLNTFHYSVVFSLSVLFFIIGNQLSKIEKISNPWVLFPLNAISVT"
BASE COUNT          473 a          287 c          244 g          419 t
ORIGIN      
        1 tgaaagacaa agcagccgga gtatcagcag aaacggcaca gcaacgtata aaagaattcc
       61 actcagaaaa attttttcac tcactccgga gcacaacaac ctttgctggc agaaaataca
      121 caaacagcga tatgccgtcg ccgaaaaaaa tgaaactaat ggcagacacc atttctgcgg
      181 tttatctcga tggatacgag ggcagacagt aaggggattt accataatcc cttaattgta
      241 cgtaccgctg aaacgctctc atacgcgttc tgacgcaaaa acgcaaaaag acaaggaaac
      301 cccaggctaa accgaaaaaa tgcgtcagaa tcgttttcag gtacttgcat aactattcac
      361 aaaagcgatg cgcgatcgat ttcgactatt ccgacggacg aacaggttaa aatgataagt
      421 ttcctccctc cggatggaag gttcagcacg gggattttta cccccttcca tcaacctgaa
      481 tatgcatctg ggagtaccca ttagtttcta ctcaaacact atcgtagtgc tatctcaaaa
      541 tataaaacat accgatggcg taagcatttt aaatatgata aataagaata tttttatcag
      601 ggattaatta tgacaacact atatatttac cttgttcttg ctgcatcatt atcggcatta
      661 tcaactgaca tgattgtgcc tgcgttacca gaactacaga aagcatttca tgctgactac
      721 tcattaattc aactaagtat tagtggattt cttattgtat ttgcatttag tcaactgata
      781 tcaggaacca taggtgatta tttcggaaaa gtacgcgtaa tgactatatg cttaattata
      841 tttcttattg gcagtattgt atgttttatg gccaatgatt taaatatgct tcttttgggt
      901 agaattctac aggcaatagg tgcaggtgcc ggacctgtaa tatcaaaagc cattgctaaa
      961 gagtcttttc caccactaaa gctaaaaaga gcactttctg acatatcatc aacaagtgcg
     1021 ataataccat taatagcacc tctggccgga gcattaatac ttgattatta taactggaat
     1081 gtaatattta ttgtaatggg tgcatttagc attctaacca tactcctgtc acctaaagag
     1141 actaataaac aggagaaaaa aacacatcaa gataatgtaa aatttgtgac taaagatttc
     1201 atctgcggga caatactcgt ttcactaatg ctaagctcac tattttgcta catatcaata
     1261 tcacctgcac tatttatgag tgattatgga ttaaatacat ttcactactc tgttgttttt
     1321 tccctgtcag ttttgttttt catcattgga aaccaattgt caaaaatcga aaaaatatca
     1381 aacccatggg tcttatttcc cctaaatgca atatcagtta cac
//
LOCUS       DAZGAN020000308         1412 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGAN020000308 DAZGAN020000000
VERSION     DAZGAN020000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.311"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1322
                     /locus_tag="ACHKYO_RS26265"
                     /old_locus_tag="pgaptmp_005253"
     CDS             <1..1322
                     /locus_tag="ACHKYO_RS26265"
                     /old_locus_tag="pgaptmp_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4431884.1"
                     /translation="GASENSARWGVDKPLYKDLISRTKAALAKNPKNRLLAVVWMQGE
                     FDIDAKPTEHSALFLAMVEKFRTDLAEKAEQCIGGRTETVPWICGDTTYFWKKKNEPA
                     YQAIYGGYKNKTDKNIHFVPLMTDENGANVPTNNPAEDPDIESIGYYGSTWRNNAATW
                     TSADRASHFSAWARRGIVSDRLATAVLTHAGRTTVKADVPSSETDAPTPSPSEAETVT
                     TTTLLSYRASESEGKLTEQGWSAGGGKAEIVADEGATGGTAMKLSKETGKGSWHLEHD
                     AGTGAELLKNGGLISCRFKASSELVANQYVIALYWPVSSLPQGVTLTGEAGNNLLAAF
                     YIQTDAKDLNVMYHNAKVATNNLKLGTFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQD
                     STPFTLTQSPVSAFAADKLHVTDITKNTTYPVLIDSIVVEVKKAVTE"
BASE COUNT          395 a          323 c          395 g          299 t
ORIGIN      
        1 cgggtgcttc agagaactca gcccgctggg gtgtggataa gccgttgtat aaggatttaa
       61 tcagccggac aaaagcggcg ctggcgaaga acccgaaaaa ccgcctgctt gccgtggtgt
      121 ggatgcaggg tgagtttgat attgatgcga agccgacgga gcattccgcg ctgtttctgg
      181 cgatggtgga aaaattccgc acagacctgg ctgaaaaagc agagcagtgt attggtggtc
      241 gaactgaaac tgtgccgtgg atttgtggcg ataccacgta tttctggaaa aagaaaaatg
      301 aaccggcata tcaggcaatc tatggcggct acaaaaataa aacagataaa aacattcatt
      361 tcgtcccatt aatgacagat gaaaatggcg cgaatgtacc caccaacaat ccggcagagg
      421 acccggatat tgaatccatt ggttattacg gctcaacgtg gcgtaacaat gctgccacct
      481 ggacatctgc ggaccgggcc agtcatttca gtgcctgggc gcgccgtggg attgtttccg
      541 accgtctggc cacggctgtt ctgactcacg cagggcgaac cacagtaaaa gccgatgttc
      601 catcttctga aactgatgcg ccaacgccgt caccttcaga agctgaaaca gtaaccacaa
      661 caacactgct gtcttaccgt gccagtgagt ctgaagggaa gctgaccgaa cagggctgga
      721 gtgcaggtgg aggtaaagcg gaaattgttg ccgatgaagg tgcaactggt ggaaccgcga
      781 tgaagctgag caaggagacg ggtaaaggat catggcatct ggagcatgat gccggtactg
      841 gcgctgaact gctgaaaaat ggcggcttaa tcagttgccg ctttaaggca tccagtgaac
      901 tggtggcaaa tcagtatgtc attgcacttt actggccggt ttcctctctg ccgcagggtg
      961 tcaccctgac aggggaagca gggaataatc tgctggcagc gttctacatc cagacagacg
     1021 caaaagacct gaatgtgatg taccacaatg caaaagtggc gacaaataac ctgaagctgg
     1081 gaacctttgg cgcatttgat aatgagtggc atacgctggc cttccgcttt gccgggaata
     1141 acagccttca ggtgacgccg gttattgatg gtcaggacag tacaccgttc acgctgacgc
     1201 agtcaccggt cagtgcattt gcggcggata aactgcatgt gacagacatt accaaaaata
     1261 cgacttaccc ggtgctgatt gacagcattg tggtggaagt gaaaaaagcg gtaacagaat
     1321 aaaaaaaatc ccgccggtca tgatatggct ggcgggagct acctaaccca taaaggaaaa
     1381 taaaaatact accgaaggag tagttatcag tc
//
LOCUS       DAZGAN020000309         1409 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGAN020000309 DAZGAN020000000
VERSION     DAZGAN020000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.312"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..630
                     /locus_tag="ACHKYO_RS26270"
                     /old_locus_tag="pgaptmp_005254"
     CDS             <1..630
                     /locus_tag="ACHKYO_RS26270"
                     /old_locus_tag="pgaptmp_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4431885.1"
                     /translation="LPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQY
                     PLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASS
                     APNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMDTKTASDYLPQTNWPT
                     TQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            627..1196
                     /gene="ybfC"
                     /locus_tag="ACHKYO_RS26275"
                     /old_locus_tag="pgaptmp_005255"
     CDS             627..1196
                     /gene="ybfC"
                     /locus_tag="ACHKYO_RS26275"
                     /old_locus_tag="pgaptmp_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HGU4431886.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHELIRSEEK"
BASE COUNT          469 a          259 c          296 g          385 t
ORIGIN      
        1 ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
       61 gacccgctgc aggggaggta tatcactcag gatccgattg ggctgaaggg gggatggaat
      121 ttttatcagt atccgctgaa tccggttcag tatatagatt caatgggact ggcatcaaaa
      181 tatggacact taaataatgg cggatatgga gcgagaccca acaaaccgcc tacgcccgat
      241 ccaagtaaat tgccggacat agcgaaacaa ttaagactgc catatcctat tgaccaggcc
      301 agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta cgactacaca
      361 ctgtattgca ggaagtgggt aaaaccaaat ctgacttgta cgccacagga tgattcccag
      421 tatccaggga tggatacaaa gacagcaagt gattacctgc cacagacaaa ttggccaaca
      481 actcaattac caccaggata tacttgtgca gaaccctatt tattcccaga cattaataaa
      541 cccgatgggc cagcaacagc agggatagat gatttgggtg aaattttagc taagatgaaa
      601 cagagaacat cgagaggaat aagaaaatga aaagagtttt gttctttttg ctgatgatat
      661 ttgttagttt tggtgttata gctgattgcg aaatacaagc taaagatcat gattgtttta
      721 ctattttcgc taagggtact attttttccg cgtttcctgt cttaaataat aaagccatgt
      781 ggagatggta tcagaatgag gacatcggtg agtattattg gcaaacagaa ctgggtacat
      841 gtaaaaataa taaattcaca ccaagcgggg cgagactctt aattcgcgtc gggtcactac
      901 gccttaatga aaatcatgcc atcaaaggaa cgttgcagga gttgataaat acagcagaaa
      961 aaacggcgtt tctcggcgat cggtttagaa gttatataag agcgggtatc tatcaaaaaa
     1021 agagcagtga tccagttcag ttgttggcag tccttgataa ttcaatcatg gtaaaatatt
     1081 ttaaagatga gaaaccaact tatgctcgaa tgacagcaca tctcccgaac aaaaatgagt
     1141 cttacgaatg tttaataaag atacagcatg aactaattcg tagtgaagag aaataaaatc
     1201 tcttcctgag aaaacaaatt aatcattgtg aaaaattata taactcatgg aataggtgtt
     1261 tattttgcgt tccatttgca gggaaagatc acgtaacgct acttttttgt actaaataat
     1321 tcgaatttta agttcaataa ttgagatact acttattgtc taaagctgtt tttcatagct
     1381 tatacatgat caaatactcc ttacataaa
//
LOCUS       DAZGAN020000310         1407 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGAN020000310 DAZGAN020000000
VERSION     DAZGAN020000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.313"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..189
                     /locus_tag="ACHKYO_RS26280"
                     /old_locus_tag="pgaptmp_005256"
     CDS             <1..189
                     /locus_tag="ACHKYO_RS26280"
                     /old_locus_tag="pgaptmp_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4431887.1"
                     /translation="EADEEPEDDVLMQKAAGLAGGVRFGPDGNEVIPASPDVADMTED
                     DVMLMTVSEGIAGGVRYG"
     gene            182..>1407
                     /locus_tag="ACHKYO_RS26285"
                     /old_locus_tag="pgaptmp_005257"
     CDS             182..>1407
                     /locus_tag="ACHKYO_RS26285"
                     /old_locus_tag="pgaptmp_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU4431888.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQ"
BASE COUNT          316 a          348 c          486 g          257 t
ORIGIN      
        1 gaggctgacg aagagcctga agatgatgtg ctgatgcaga aagcggcagg gcttgccgga
       61 ggcgttcgtt ttggcccgga cgggaatgaa gttatccccg cttccccgga tgtggcggac
      121 atgacggagg atgacgtaat gctgatgaca gtatcagaag ggatcgcagg aggagtccgg
      181 tatggctgaa ccggtaggcg atctggtcgt tgatttaagt ctggatgcgg ccagatttga
      241 cgagcagatg gccagagtca ggcgtcattt ttccggtacg gaaactgatg cgaaaaaaac
      301 agcggcagtc gttgaacagt cgctgagccg acaggcgctg gctgcacaga aagcggggat
      361 ttccgtcggg cagtataaag ccgccatgcg tatgctgcct gcacagttca ccgacgtggc
      421 cacgcagctt gcaggcgggc aaagcccgtg gctgatcctg ctgcaacagg gtggtcaggt
      481 taaggactcc ttcggcggga tgatccccat gttcaggggg cttgccggtg cgatcaccct
      541 gccgatggtg ggggccacct cgctggcggt ggcgaccggt gcgctggcgt atgcctggta
      601 tcagggcaac tcaaccctgt ccgatttcaa caaaacgctg gtcctttccg gcaatcaggc
      661 gggactgacg gcagatcgta tgctggtcct gtccagagcc gggcaggcgg cagggctgac
      721 gtttaaccag accagcgagt cactgacggc gctggtgaat gccggtgtgc gtggtggtga
      781 gcagtttgag gcgatcagcc agagtgtggc gcgtttctcc tctgcatccg gcgtggaggt
      841 ggacaaggtc gctgaagcct tcgggaagct gaccacagac ccgacgtcgg gactgacagc
      901 gatggcacgt cagttccata acgtgacggc ggagcagatt gcgtatgttg ctcagttgca
      961 gcgttccgga gatgaagccg gggcattgca ggcggcgaac gaggccgcaa cgaaagggtt
     1021 tgatgaccag acccgccgcc tgaaagagaa catgggcacg ctggagacct gggcagacag
     1081 gacagcacgg gcattcaaat ccatgtggga ttcggtgctg gatattggtc gcccggacac
     1141 tgcccaggga atgctggaga aagcagaaaa ggcttttgat gaggcggaca aaaaatggca
     1201 gtggtatcag agccggagcc accggcgcgg taaaacctca gcatttcttg ccaatctccg
     1261 gggagcatgg gaggacagag cgaatgcgca acttgggctt tcagccgcca cgttgcaggc
     1321 cgatcttgaa aaggccagag agatggcagc aaaggactgg gccgagtctg aggcatcacg
     1381 gctgaaatat accgaagagg cgcagaa
//
LOCUS       DAZGAN020000311         1396 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGAN020000311 DAZGAN020000000
VERSION     DAZGAN020000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.314"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..395)
                     /locus_tag="ACHKYO_RS26290"
                     /old_locus_tag="pgaptmp_005258"
     CDS             complement(<1..395)
                     /locus_tag="ACHKYO_RS26290"
                     /old_locus_tag="pgaptmp_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HGU4431889.1"
                     /translation="MLVSKSNGFNASAFLGSGSYNENKSSKHMELLAHSILKLICKEA
                     ASETYRGALETLQKMMSECIYQEGNAFVIMGAGEQLKRIKYEVGENNLKVFNVHFNNN
                     HELVSSGEPDVICLSKQVWENLLIKLKLE"
     gene            complement(1147..1222)
                     /locus_tag="ACHKYO_RS26295"
                     /old_locus_tag="pgaptmp_005259"
     tRNA            complement(1147..1222)
                     /locus_tag="ACHKYO_RS26295"
                     /old_locus_tag="pgaptmp_005259"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1187..1189),aa:Ile,seq:cat)
BASE COUNT          419 a          291 c          229 g          457 t
ORIGIN      
        1 ttttccagct ttagtttaat gagaagattt tcccagacct gcttgcttaa acatattacg
       61 tcaggctcac cagaactaac taactcgtga ttattattaa agtgtacgtt gaataccttt
      121 aagttatttt caccaacttc atatttaata cgttttaatt gttctccagc tcccataatg
      181 acaaaggcgt tgccttcttg atatatacat tcagacatca ttttttgtaa agtttcaaga
      241 gcaccgcgat acgtctctga tgcagcttcc ttacaaatta attttaaaat actatgagct
      301 agtagctcca tgtgtttaga agatttattt tcattataac ttccactacc caaaaatgcg
      361 ctagcgttaa atccgttgct tttactaact aacattgtct attcctcaat taatgtctac
      421 atggctattt ttaattttat tattgtttgt cactataaaa aatcgctcat ttgagacaat
      481 tgctggcatt aacagcttca tatgctatac atggtacttt taattaaatt agcacaagaa
      541 tgttaaattt aataaacaaa aagttatttc gctgtatgat aaaaaccacc cgttataatt
      601 tattagtgaa attcgttttt cgagtgttag aaatttatat ctcaatagcg ttggttaatg
      661 agcatagcca cgctcctgta acgctcacaa aactcatctg cctgcggcgg gtgttctggt
      721 cagtagtaga tgtttaaggc gtggcagaga catttcatcc ttactctacg gcattgttct
      781 acatacattg gttgtggtac tcactcatca tcagtgagcg aacagagaat agttcagtga
      841 tttgagtaat taacctgatt aaattaaggg gtataataaa tgataatact ctggctttat
      901 cgttaattac ttaattccac atgtaagcaa tttgcccgct tggcatagca ggcatttttt
      961 ccaggtactt ttgaatgagt actgatggat aaatacattg cagtggcgtg ccacgtacca
     1021 aaacaccagc cctcattcga aaccacccac cgcacttctt ccttgaaatg gcgttagtca
     1081 tgaaatatag accgccatcg agtacccctt gtacccttaa ctcttcctga tacgtaaata
     1141 atgatttggt ggcccttgct ggacttgaac cagcgaccaa gcgattatga gtcgcctgct
     1201 ctaaccactg agctaaaggg ccttgagtgt gcaataacaa tacttataaa ccacgcaata
     1261 aacatgatga tcatatgatg taaataacag atttttatgc gttctcattc atccttgctc
     1321 gtcattacac tcgacataaa acccgacacc gcttccattc gcaaagtcga tactcgcagt
     1381 caacaagcaa atgtta
//
LOCUS       DAZGAN020000312         1383 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGAN020000312 DAZGAN020000000
VERSION     DAZGAN020000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.315"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..111
                     /locus_tag="ACHKYO_RS26300"
                     /old_locus_tag="pgaptmp_005260"
     CDS             <1..111
                     /locus_tag="ACHKYO_RS26300"
                     /old_locus_tag="pgaptmp_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HGU4431890.1"
                     /translation="DCGGEIPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            92..400
                     /locus_tag="ACHKYO_RS26305"
                     /old_locus_tag="pgaptmp_005261"
     CDS             92..400
                     /locus_tag="ACHKYO_RS26305"
                     /old_locus_tag="pgaptmp_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HGU4431891.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            397..687
                     /locus_tag="ACHKYO_RS26310"
                     /old_locus_tag="pgaptmp_005262"
     CDS             397..687
                     /locus_tag="ACHKYO_RS26310"
                     /old_locus_tag="pgaptmp_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HGU4431892.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRASVPGVKKPRARG"
     gene            690..1271
                     /locus_tag="ACHKYO_RS26315"
                     /old_locus_tag="pgaptmp_005263"
     CDS             690..1271
                     /locus_tag="ACHKYO_RS26315"
                     /old_locus_tag="pgaptmp_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HGU4431893.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            1271..>1383
                     /locus_tag="ACHKYO_RS26320"
                     /old_locus_tag="pgaptmp_005264"
     CDS             1271..>1383
                     /locus_tag="ACHKYO_RS26320"
                     /old_locus_tag="pgaptmp_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431894.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSL"
BASE COUNT          319 a          381 c          417 g          266 t
ORIGIN      
        1 gactgtggcg gagagatccc cgccgcacgt cttgttgctg tgccggatgc catccgttgt
       61 gtgaactgcc agaacatcat ggaggcccgt catgtgggtc agcatcgtta aagattatgt
      121 tgtgccgatc ctttcggcga cggcaaccgc tggcgggata ttcatggcgc tgatgcgcaa
      181 aacgtttgtc ccccgcgagg cctttgaaaa actctccgac cgcgttgaga aggtggaaac
      241 ccgcctgtcg tcactgccga cagaagccga ggtcaaccgt ctgaatgtgg aaatcgtgac
      301 cctgcggggt gaactgaaaa ccacgaacgc cacgctccgc tctgtctcct atcagaacga
      361 actgctgctg gagcaggctg taaggaaaaa aacgcaatga gtgatttcat tactgaagat
      421 cagcgtctgg tcattctgcg gtcgctggca gattacaacg gtgaactggg tgaatccgtg
      481 ctccaggact gtctggatga ttacggccac cgcgtgtccc gcgacaccgt tcacacccac
      541 attgcctggc ttgccgagca gggactggtg cgtaagcgca cgctcgttaa cggttatttc
      601 atcgccgaac tgaccggccg gggtcaggat gtggcggaag gtcgcgccag cgttccgggt
      661 gtgaagaaac cacgcgcaag ggggtaacga tggacaagcc gacgcgtgga cgcgtgcgca
      721 aggtggattt gctccccgac agcatccgta agccactgct ggaaatgctg cgtgaaaaac
      781 gtctgacgca ggtccagata cgcgaggaaa tcaaccgtct gatccgtgag gcgggcctgc
      841 cggaagagca gcagctttca ccggcggcca tcagccggga agcctcccgt aatgagctga
      901 ttgcccgtaa cctgcgtgat ttacgcgagc agacaaaagc catgatggcc gaactgggag
      961 acaagccaac cggtgaaacc acagcgctga ttctggagat gtcccgcgcc ctgatgtacc
     1021 gccgtctgcg tgccgcgacc gaatccctga acagtgacag cgacgtggat atgcgtctta
     1081 ttaaggacat cctcctgtca gcacagcgtg cggaaagtgc cgccgagcgc agcattaagc
     1141 gtgaaaaaga aatccgggcc gcatttgctg aagagatggc aaacgccgtc accgacgagc
     1201 tgcgcggcgt ggacgggatg agtgaacagc ttgagtcccg tattaagggg attctgttgg
     1261 gtaaagcctg atgaataacg ccgaagaact ctccccctta ctgacgaata ccgtctccac
     1321 ccgcaaaatt gatctggccg gtgaaaaagc cctgctgggc gtggatgtgc cggacagcct
     1381 tga
//
LOCUS       DAZGAN020000313         1378 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZGAN020000313 DAZGAN020000000
VERSION     DAZGAN020000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.316"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..943
                     /locus_tag="ACHKYO_RS26325"
                     /old_locus_tag="pgaptmp_005265"
     CDS             <1..943
                     /locus_tag="ACHKYO_RS26325"
                     /old_locus_tag="pgaptmp_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4431895.1"
                     /translation="QLAALGDKVTYQERLNALAQQADKFAQQQRAKRAAIDAKSRGLT
                     DRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQLRGSWMAGLKSGWSEWEE
                     SATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGI
                     VGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVF
                     TKEATSRIGVGNLYRLMRGYATGGYVGTPGSLADSRSQASGTFEQNNHVVINNDGTNG
                     QIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            940..1269
                     /locus_tag="ACHKYO_RS26330"
                     /old_locus_tag="pgaptmp_005266"
     CDS             940..1269
                     /locus_tag="ACHKYO_RS26330"
                     /old_locus_tag="pgaptmp_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431896.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            1269..>1378
                     /locus_tag="ACHKYO_RS26335"
                     /old_locus_tag="pgaptmp_005267"
     CDS             1269..>1378
                     /locus_tag="ACHKYO_RS26335"
                     /old_locus_tag="pgaptmp_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4431897.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERY"
BASE COUNT          315 a          328 c          472 g          263 t
ORIGIN      
        1 ccagctggct gcacttggcg acaaggttac gtatcaggag cgcctgaacg cgctggcgca
       61 gcaggcggat aaattcgcac agcagcaacg ggcaaaacgg gccgccattg atgcgaaaag
      121 ccgggggctg actgaccggc aggcagaacg ggaagccacg gaacagcgcc tgaaggaaca
      181 gtatggcgat aatccgctgg cgctgaataa cgtcatgtca gagcagaaaa agacctgggc
      241 ggctgaagac cagcttcgcg ggagctggat ggcaggcctg aagtccggct ggagtgagtg
      301 ggaagagagc gccacggaca gtatgtcgca ggtaaaaagt gcagccacgc agacctttga
      361 tggtattgca cagaatatgg cggcgatgct gaccggcagt gagcagaact ggcgcagctt
      421 cacccgttcc gtgctgtcca tgatgacaga aattctgctt aagcaggcaa tggtggggat
      481 tgtcgggagt atcggcagcg ctattggcgg ggctgttggt ggcggcgcat ccgcgtcagg
      541 cggtacagcc attcaggccg ctgcggcgaa attccatttt gcaaccggag gatttacggg
      601 aaccggcggc aaatatgagc cagcggggat tgttcaccgt ggtgaatttg tcttcacaaa
      661 ggaggcaacc agccggattg gcgtggggaa tctctaccgg ctgatgcgcg gctatgccac
      721 cggtggttat gtcggtacac cgggcagtct ggctgacagc cggtcgcagg cgtccgggac
      781 gtttgagcag aataaccatg tggtgattaa caacgacggc acgaacgggc agataggtcc
      841 ggctgctctg aaggcggtgt atgacatggc ccgcaagggt gcccgtgatg aaattcagac
      901 acagatgcgt gatggtggac tgttctccgg aggtggacga tgaaaacctt ccgctggaaa
      961 gtgaaacccg gtatggatgt ggcttcggcc ccttctgtaa gaaaggtgcg ctttggtgat
     1021 ggctattccc agcgagcgcc tgccgggctg aatgccaacc tgaaaacgta cagcgtgacg
     1081 ctttctgtcc cccgtgagga ggccacggta ctggagtcgt ttctggaaga gcacgggggc
     1141 tggaaagcct ttctgtggac gccgccttat gagtggcggc agataaaggt gacctgcgca
     1201 aaatggtcgt cgcgggtcag tatgctgcgt gttgagttca gcgcagagtt tgaacaggtg
     1261 gtgaactgat gcaggatatc cggcaggaaa cactgaatga atgcacccgt gcggagcagt
     1321 cggccagcgt ggtgctctgg gaaatcgacc tgacagaggt cggtggagaa cgttattt
//
LOCUS       DAZGAN020000314         1371 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZGAN020000314 DAZGAN020000000
VERSION     DAZGAN020000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.317"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1106
                     /locus_tag="ACHKYO_RS26340"
                     /old_locus_tag="pgaptmp_005268"
     CDS             <1..1106
                     /locus_tag="ACHKYO_RS26340"
                     /old_locus_tag="pgaptmp_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4431898.1"
                     /translation="TWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREK
                     AQRRSLAEKLQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTV
                     EQLARQVEPEYTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYY
                     LHQPYRLPGQQHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRM
                     DPLGLYNLYQLLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDI
                     CIYATACGHAGIGGGINAAITYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVD
                     NPESSTESVGIGAGVDASVMTLACRTWQECWVN"
     gene            1118..1303
                     /locus_tag="ACHKYO_RS26345"
                     /old_locus_tag="pgaptmp_005269"
     CDS             1118..1303
                     /locus_tag="ACHKYO_RS26345"
                     /old_locus_tag="pgaptmp_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431899.1"
                     /translation="MKKYIFLVLFLLCGCSPVDIEPARQEVNIFRGLYQTGKYSDIYK
                     ITSSDFQTATSEKNLST"
BASE COUNT          385 a          269 c          393 g          324 t
ORIGIN      
        1 tgacgtggta cgggtgggac ggcgaccggc tgacgacggt acagaccgac accacgcgta
       61 tccagacggt atatcagccg gggagcttcg cgccgctcat ccgcatcgaa acagacaacg
      121 gcgagcggga gaaagcgcag cgccgcagcc tggcggagaa gctgcagcag gaagggagcg
      181 aggacgggca cggcgtggta tttccggctg aactggtgcg gctgctggac aggctggagg
      241 aagaaatccg ggcagaccgc gtgagcagtg aaagccgggc gtggcttgcg cagtgcgggc
      301 tgacggtgga gcaactggcc agacaggtgg agccggaata cacaccggcg cgaaaagttc
      361 atttttacca ctgcgaccac cggggcctgc cgctggcgct cattagcgaa gacggcaata
      421 cggcgtggcg cggggagtat gatgaatggg gcaaccagct taatgaagag aacccgtatt
      481 acctgcacca gccataccgt ctgccggggc agcagcatga tgaggaatca gggctgtact
      541 ataaccggaa ccggtactat gacccgctac aggggaggta tattacacaa gaccccattg
      601 ggctggcggg gggatggaac ctgtataatt acccactgaa tccgataata aggatggatc
      661 ctttgggttt gtataattta tatcaattat tatatgatgt ttggcatgat gattcatatg
      721 gaacatcatc aattgatatt actggcagtg gagatctaat atcattaggt ggtcatgcag
      781 gacttggcgt tgcgtttgct aaaaagaaag gtgaaatgtt atctgatatt tgtatttatg
      841 ctacagcatg cggacatgca ggaattggtg gtgggataaa tgcggctatc acatattctg
      901 agaccaagtc tttacctaca tcgggagtca gcaattcagt aggtgtaacg gttggcggcg
      961 gagttggggg gcattttgcg tatacttatg tagtggatgt tgataatcca gaatcatcga
     1021 cagaatctgt tggtatcggt gcaggtgttg acgcttcagt tatgactctg gcttgtagaa
     1081 cgtggcaaga atgctgggtc aattaacagg aggaaatatg aaaaaatata tatttttagt
     1141 tttattttta ttgtgtggtt gttctccagt ggacatagag ccagctagac aagaagttaa
     1201 tatctttcgt ggtttatatc agacagggaa atacagtgat atatataaaa tcacttcaag
     1261 tgattttcag actgccacat ctgaaaaaaa tttatcgaca taatgaacga agcaaaagaa
     1321 aaagatcttg ggacttataa aaaatcaacc cttaaaactg aaaaaatcac t
//
LOCUS       DAZGAN020000315         1352 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZGAN020000315 DAZGAN020000000
VERSION     DAZGAN020000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.318"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..152
                     /locus_tag="ACHKYO_RS26350"
                     /old_locus_tag="pgaptmp_005270"
     CDS             <1..152
                     /locus_tag="ACHKYO_RS26350"
                     /old_locus_tag="pgaptmp_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4431900.1"
                     /translation="KAAGLAGGVRFGPDVNEVIPVSPDEADMTEDDVMLMTVSEGIAG
                     GVRYG"
     gene            145..>1352
                     /locus_tag="ACHKYO_RS26355"
                     /old_locus_tag="pgaptmp_005271"
     CDS             145..>1352
                     /locus_tag="ACHKYO_RS26355"
                     /old_locus_tag="pgaptmp_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU4431901.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSSTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQS
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWSLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQ"
BASE COUNT          307 a          332 c          459 g          254 t
ORIGIN      
        1 agaaagcggc agggcttgcc ggaggcgtcc gctttggccc ggacgtgaat gaagttatcc
       61 ccgtttcccc ggatgaggcg gacatgacgg aggatgacgt aatgctgatg acagtatcag
      121 aagggatcgc aggaggagtc cggtatggct gaaccggtag gcgatctggt cgttgattta
      181 agtctggatg cggccagatt tgacgagcag atggccagag tcaggcgtca tttttccagt
      241 acggaaagtg atgcgaaaaa aacagcggca gtcgttgaac agtcgctgag ccgacaggcg
      301 ctggctgcac agaaagcggg gatttccgtc gggcagtata aagctgccat gcgtatgctg
      361 cctgcgcagt tcaccgacgt ggccacgcag cttgcaggcg ggcaaagtcc gtggctgatc
      421 ctgctgcaac agggtggtca ggtgaaggac tccttcggcg ggatgatccc catgttcagg
      481 gggcttgccg gtgcgatcac cctgccgatg gtcggggcca cctcgctggc ggtggcgacc
      541 ggtgcgctgg cgtatgcctg gtatcagggc aactcaaccc tgtccgattt caacaaaacg
      601 ctggtccttt ccggcaatca gtcgggtctg acggcagatc gtatgctggt cctgtccaga
      661 gccgggcagg cggcagggct gacgtttaac cagaccagcg agtcactcag cgcactggtt
      721 aaggcgggag taagcggtga ggctcagatt gcatccatca gccagagtgt ggcgcgtttc
      781 tcctctgcat ccggcgtgga ggtggacaag gtcgctgaag ccttcgggaa gctgaccaca
      841 gacccgacgt cagggctgac agcgatggca cgccagttcc ataacgtgac ggcggagcag
      901 attgcgtatg ttgctcagtt gcagcgttcc ggcgatgaag ccggggcatt gcaggcggcg
      961 aacgaggccg cgacgaaagg gtttgatgac cagacccgcc gcctgaaaga gaacatgggt
     1021 acgctggaga cctgggcaga caggacagcg cgggcattca aatccatgtg ggatgcggtg
     1081 ctggatattg gtcgtcctga taccgctcag gagatgctga ttaaggcaga ggctgcgttt
     1141 aagaaagcgg acgacatctg gagtctgcgc aaggatgatt attttgttaa cgatgaagcg
     1201 cgggcgcgtt actgggatga tcgtgaaaag gcccgtcttg cgcttgaagc cgcgagaaag
     1261 aaggctgaac agcagagtca acaggacaaa aatgcgcagc agcagagcga tactgaagcg
     1321 tcacggctga aatataccga agaggcgcag aa
//
LOCUS       DAZGAN020000316         1340 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZGAN020000316 DAZGAN020000000
VERSION     DAZGAN020000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.319"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..204)
                     /locus_tag="ACHKYO_RS26360"
                     /old_locus_tag="pgaptmp_005272"
     CDS             complement(<1..204)
                     /locus_tag="ACHKYO_RS26360"
                     /old_locus_tag="pgaptmp_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431902.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYSGEFFCRITTRDG"
     gene            complement(194..391)
                     /locus_tag="ACHKYO_RS26365"
                     /old_locus_tag="pgaptmp_005273"
     CDS             complement(194..391)
                     /locus_tag="ACHKYO_RS26365"
                     /old_locus_tag="pgaptmp_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431903.1"
                     /translation="MNSLPAGWARPLMARKHHFFKTGENISICGRWLYLAHNREPDTF
                     ESPDDCAECRRRVNKEKDNGQ"
     gene            complement(384..716)
                     /locus_tag="ACHKYO_RS26370"
                     /old_locus_tag="pgaptmp_005274"
     CDS             complement(384..716)
                     /locus_tag="ACHKYO_RS26370"
                     /old_locus_tag="pgaptmp_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431904.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKIVSFMDKECDKRDMPEI
                     IMDDMILHSAVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            complement(732..1082)
                     /locus_tag="ACHKYO_RS26375"
                     /old_locus_tag="pgaptmp_005275"
     CDS             complement(732..1082)
                     /locus_tag="ACHKYO_RS26375"
                     /old_locus_tag="pgaptmp_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431905.1"
                     /translation="MRSYIDNEKLKTISDCLSLLAKIKETIEEIKFQLEYEPCGDDTW
                     RNSARKALAVFQKQRRTVEYRLAVLRQEEKERNIRRHELVNDFLVRELKERVPESVFF
                     ECEAVARSKALETD"
     gene            complement(1097..>1340)
                     /locus_tag="ACHKYO_RS26380"
                     /old_locus_tag="pgaptmp_005276"
     CDS             complement(1097..>1340)
                     /locus_tag="ACHKYO_RS26380"
                     /old_locus_tag="pgaptmp_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431906.1"
                     /translation="GTLTGVYFGDLRGYPWHSLDDAFPPDMEAVVLIVQYGDQQELRI
                     GHMGYEGFFIDEETGACLEDEDGQVTHWCHISALPE"
BASE COUNT          362 a          344 c          252 g          382 t
ORIGIN      
        1 gccatctctg gttgttatcc ggcagaaaaa ttcaccgcta tataacacaa tggcctgctg
       61 aatgagcgca tcagccgccc gttcaaccgg atgtctttta tcaaaaaagc agatgatttt
      121 tcgacataac caccgggtaa taacaaatgc gccgaaaaag gatgtgatat ataagcacca
      181 gaacaacaga gaactattgt ccattatctt tctccttatt cactcttctg cggcattcgg
      241 cacagtcatc agggctttca aatgtatccg gctcgcgatt atgagccaga tataaccacc
      301 gtccacatat actgatattt tcgcctgttt taaaaaaatg atgcttcctg gccattagtg
      361 gccgcgccca tccagcgggt agactattca tttgctttat cctccatgct ggacagaatc
      421 atatgtatta atgtcgggcg tggagatata cgcatttcat taagcgtctg aagggcagca
      481 acagctgcct gtttatcggt gcctttggca atttcagcaa gagaatgcac aatccataac
      541 gggcttaacg ctacggctga atgcagaatc atgtcatcca taataatttc tggcatatcc
      601 cgtttgtcac attctttatc cataaaagaa acgatttttt ctgcatcttc cggtttcatg
      661 cctccggcga tgagaatttt acgatattca taagacgatc ttaattcaat actcatcatt
      721 cacctgcctg tttaatcagt ttccagtgct ttgctacggg caacagcctc acactcaaaa
      781 aataccgact ccggcacacg ctcctttaat tcacgaacaa gaaaatcatt aacgagttca
      841 tgacgtctta tatttctttc cttttcctcc tggcgcaata cagcaaggcg gtattcaaca
      901 gtccggcgtt gtttctgaaa gacagccagt gcttttctgg cagagttacg ccatgtatca
      961 tcgccacagg gttcgtattc aagctgaaat ttaatctctt caatggtttc ttttattttt
     1021 gccagcaagc taagacagtc gctgattgtc ttcagttttt cattatcaat ataacttctc
     1081 acgcattcac ctcccgtcat tccggtaaag ctgaaatatg gcaccagtgc gtcacctgtc
     1141 cgtcctcatc ttcaaggcag gctccggttt cttcatcaat aaaaaagcct tcatacccca
     1201 tatggccgat gcgcagttcc tgttggtcac catactgaac aatcagcacg acagcctcca
     1261 tatcaggcgg aaatgcgtca tccagtgaat gccagggata accgcgcaaa tccccgaaat
     1321 aaacaccggt cagcgttccg
//
LOCUS       DAZGAN020000317         1330 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGAN020000317 DAZGAN020000000
VERSION     DAZGAN020000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.320"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            336..770
                     /locus_tag="ACHKYO_RS26385"
                     /old_locus_tag="pgaptmp_005277"
     CDS             336..770
                     /locus_tag="ACHKYO_RS26385"
                     /old_locus_tag="pgaptmp_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HGU4431907.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            863..1129
                     /locus_tag="ACHKYO_RS26390"
                     /old_locus_tag="pgaptmp_005278"
     CDS             863..1129
                     /locus_tag="ACHKYO_RS26390"
                     /old_locus_tag="pgaptmp_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431908.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            1194..>1330
                     /locus_tag="ACHKYO_RS26395"
                     /old_locus_tag="pgaptmp_005279"
     CDS             1194..>1330
                     /locus_tag="ACHKYO_RS26395"
                     /old_locus_tag="pgaptmp_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGU4431909.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LS"
BASE COUNT          281 a          400 c          391 g          258 t
ORIGIN      
        1 aggaaactgc gcctgatggc gcagttgtct gcccttccgc cgccacccct gcggcctgtt
       61 tcccgctgtg gactttccgg gggctgaagc ggccacaaca cgtgtcagcc gtccaggggg
      121 gagatgtacc agccacaaaa ctttttgccg caagcgtcaa aaacttttgc gtctgccccc
      181 ggacaactgc cccgtgccgt ggctgtcgct gccccatcgg aaaggtccac tgcggggaga
      241 aaccccgaag aagtggacgg cagaaggggc tttcgcccct ccgggggagc aaccggtcgg
      301 cccggcgacc tgattcagca gaaggagacg ttttcatgac agtcagcagc acgatttccg
      361 ttttttgccg cgacggggtg ttccgtaccg tttactgcca cctgcacggt gagccgacct
      421 ggaacggtcg catcctgcat acccactatg ccaccggtca gcaggccgaa gccctggttg
      481 aacacggtga tatccgttgc ctcggtcccc gttgcgacaa acccgccggg catacgcttc
      541 agaacccggt ggacggtgtg acggcttatt acggacgtga cagtggtttc cggatggaca
      601 gtgaggcgcg tgagtaccgt tctttcatgg aggctattgc cacggaaagc acagaagagg
      661 tgcgcttcca ttatgtgttc atcgacggct actggaaggt gatgtaccgc acgccggaag
      721 gctggaagat gaaagcgctc gcgctggcac tgcgtcgctg tccggaatga aaaaaagcag
      781 ggcgataaac cctgcctttc tccgccggcg cttccccgta cggaagtccg gcatctcatt
      841 cactaactat ggagattatg ccatgagacc atcaattatc ttcgcaaccg ccgagtatgt
      901 aaagcgtctg cgtgaagagt gcctgcggga gaacaaaccc ctgcaccgcc atacccgctt
      961 cagacgccag gaactggcac cggatgagat taacccggac gtcctggcga tgggcggtca
     1021 tatcgccaga cgctgcagtg agcagaagcg ggtacgtatc cccgccatga aagtcagcga
     1081 atggggccac ctgctccgcg cgcttgaaat tgagcgggtc tgccactgat taacccttgc
     1141 cgtctgcctg tgcaggcggc aggatacgtt cagacatctc accaggagga accgtgagta
     1201 agaagaaaaa caccacaacg cccacgccgc atgatgccgc gttccggtcg ttcctggcga
     1261 atcccgacgt cgccagagat tttctggaac tgcatcttcc ggcggagtac cggcagttgt
     1321 gcgacctgtc
//
LOCUS       DAZGAN020000318         1321 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGAN020000318 DAZGAN020000000
VERSION     DAZGAN020000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.321"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            122..1219
                     /locus_tag="ACHKYO_RS26400"
                     /old_locus_tag="pgaptmp_005280"
     CDS             122..1219
                     /locus_tag="ACHKYO_RS26400"
                     /old_locus_tag="pgaptmp_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU4431910.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
BASE COUNT          383 a          283 c          285 g          370 t
ORIGIN      
        1 ctacttcaca atttaaatga gaaactaaaa cttacatctt gaaataatct cattgattgg
       61 atgaatattt atagtgcagt gacatcattt ttaaataata gttcaaataa aagggctcac
      121 gatgaaaaaa ttaacagtgg caatttctgc tgtagctgca tcagtactga tggcgatgtc
      181 tgctcaggca gctgaaattt ataataaaga cagtaacaag ctggatctgt acgggaaagt
      241 taatgccaag cactactttt cctctaacga tgcagatgat ggtgatacta cttatgttcg
      301 tctgggcttc aaaggcgaaa cccaaatcaa cgatcagctg actggtttcg gtcagtggga
      361 atatgaattc aaaggcaacc gcgctgaatc tcaaggttcc tccaaagaca aaacccgtct
      421 tgcatttgca ggcctgaaat tcggtgacta cggctcaatc gattacggcc gtaactacgg
      481 tgtagcatac gacatcggtg cgtggactga cgttctgcca gaattcggtg gcgatacctg
      541 gacccaaaca gatgtgttca tgactggtcg caccactggt gttgcaacct atcgtaacaa
      601 cgacttcttt ggtctggttg atggtctgaa ctttgctgct cagtaccaag gcaaaaacga
      661 tcgtagcgat ttcgataact acaccgaagg taacggtgat ggcttcggtt tctctgctac
      721 ctatgaatac gaaggattcg gtatcggtgc aacttatgcg aaatctgatc gtaccgacac
      781 tcaagttaat gcagggaaag ttcttcctga agtatttgct tccggtaaaa atgcagaagt
      841 ttgggccgca ggtctgaaat atgacgctaa caacatttac ctggccacta cctattctga
      901 aacccagaat atgactgtat ttgctgatca cttcgttgct aataaagctc aaaacttcga
      961 agctgttgca caatatcagt tcgatttcgg tctgcgtccg tccgttgctt acctgcaatc
     1021 taaaggtaag gatcttggag tatggggcga tcaggactta gtcaaatatg ttgatgtagg
     1081 tgcaacctat tacttcaaca aaaatatgtc tactttcgtt gattacaaaa tcaacctgct
     1141 tgacaaaaat gacttcacta aagcactcgg tgtaagcact gatgacatcg ttgctgtagg
     1201 tctggtttac cagttctaat ctgattacga aaaagatatg ttgcgggagg cgttgcctcc
     1261 ccaacatata agtggctccc tcaagccact tcctttagga gcacaacctt gcttctaact
     1321 a
//
LOCUS       DAZGAN020000319         1318 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGAN020000319 DAZGAN020000000
VERSION     DAZGAN020000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.326"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(46..459)
                     /locus_tag="ACHKYO_RS26405"
                     /old_locus_tag="pgaptmp_005281"
     CDS             complement(46..459)
                     /locus_tag="ACHKYO_RS26405"
                     /old_locus_tag="pgaptmp_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431911.1"
                     /translation="MKKFMLLIFSVSFFLTGCATIVGDKTQTVQVNSNPSGADFIIKD
                     ESGKTISSGKTPQNVTLQKSDGSYFGKKSYQITFSKENYQPVTLPIKASANGWYIGGN
                     FVFGGIVGWLLVDPFNGGMYTLSPESLNPGLVPKT"
     gene            complement(523..732)
                     /locus_tag="ACHKYO_RS26410"
                     /old_locus_tag="pgaptmp_005282"
     CDS             complement(523..732)
                     /locus_tag="ACHKYO_RS26410"
                     /old_locus_tag="pgaptmp_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HGU4431912.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEPDPLAALEDALLFASEN"
     gene            complement(828..1202)
                     /locus_tag="ACHKYO_RS26415"
                     /old_locus_tag="pgaptmp_005283"
     CDS             complement(828..1202)
                     /locus_tag="ACHKYO_RS26415"
                     /old_locus_tag="pgaptmp_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU4431913.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(1217..>1318)
                     /locus_tag="ACHKYO_RS26420"
                     /old_locus_tag="pgaptmp_005284"
     CDS             complement(1217..>1318)
                     /locus_tag="ACHKYO_RS26420"
                     /old_locus_tag="pgaptmp_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431914.1"
                     /translation="DAGKYTCVVTDSAEKAQSVTSVECTVTVSSAAG"
BASE COUNT          325 a          399 c          274 g          320 t
ORIGIN      
        1 aaaaaaacct gccgcagcag gttttattca tcagcagatt attgattaag ttttgggtac
       61 cagcccgggg ttcagacttt ccggactcag tgtatacata ccgccattga atgggtccac
      121 aagcaaccag ccaacaatac caccaaagac aaagttacca ccaatgtacc atccattagc
      181 cgatgccttg atcggcagcg ttacgggctg ataattctct ttactgaatg tgatctgata
      241 actctttttc ccgaagtaac tgccgtcaga tttttgaagt gttacattct gcggtgtttt
      301 gcccgaagaa attgttttcc ctgactcatc tttaataata aaatccgcac cagacggatt
      361 actgtttacc tgtacagtct gcgttttatc tccgacaatg gttgcacacc ccgtcaggaa
      421 aaagctgaca gagaaaatca acagcatgaa tttcttcata aaatggctcc gttcagttaa
      481 ttttccccga aaatactgta ataaagatct gcaaagttaa aattaatttt ccgaagcaaa
      541 taacagagca tcctccagag cggccaaagg atctggctcc ggcttatcct catcctcgcc
      601 ccagcagagc atggcgtcct tcagtgcaac attcatcccc tgtgccccga aaaccgcttt
      661 cacgatctgt gcattacgga tatccccgcg ctcatcaccc agcggggata ccctgtcgaa
      721 ctccatccac atcatcgcct cgctcgcact caggctgtgc cgcagttcgg ataaggtgcg
      781 ccccagacgg agcgcaagtc gcatcagaaa gcgaatttcc gggcgggcta cttttttctg
      841 gccgactctg catcagcgat cagttccagt gcctgacgca gcaaccgggc atgtaccgga
      901 ccatagacgg ccagcacctg ctcacggtcg tccggagtga acacccgctg caggtccgta
      961 tcacacagga catcgcagaa cagcgtcaca tccgcttcca ggttacggcg ggttttcgcc
     1021 accaccgaca gggtatcgtc atcctctcca tcaccattga gcacgtcctg ccacagatac
     1081 caggcctctg ccgaaggctc ccgcagcacc acgctgacat taccccattc cggcaccttc
     1141 accgttttat gacgaaaccc tgacagtctg gccagcgcca gcgttttcag atcctttttc
     1201 atgatgaccc atccccttat ccggcggctg agctcactgt cactgtacat tcaacagacg
     1261 tcacactctg tgctttctct gccgaatcgg tcaccacgca ggtatatttt cccgcatc
//
LOCUS       DAZGAN020000320         1312 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGAN020000320 DAZGAN020000000
VERSION     DAZGAN020000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.327"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            30..764
                     /gene="nanR"
                     /locus_tag="ACHKYO_RS26425"
                     /old_locus_tag="pgaptmp_005285"
     CDS             30..764
                     /gene="nanR"
                     /locus_tag="ACHKYO_RS26425"
                     /old_locus_tag="pgaptmp_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HGU4431915.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(800..>1312)
                     /locus_tag="ACHKYO_RS26430"
                     /old_locus_tag="pgaptmp_005286"
     CDS             complement(800..>1312)
                     /locus_tag="ACHKYO_RS26430"
                     /old_locus_tag="pgaptmp_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4431916.1"
                     /translation="KNADKWHLQRHRIGVLGFSAGGHLASTFVNQICDFKRSSDSKEQ
                     EIFAIPDFVGLIYPVISMKDDVTHPDSRKQLLGDNSTSENIIQYSADLNVTSCFPPVF
                     LLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHLYEKGGHGFSVQWATDLPVGGWIKLF
                     MDWMYSHGFK"
BASE COUNT          455 a          277 c          235 g          345 t
ORIGIN      
        1 catttgctct aatccatata aatcaatata tgtccgacaa caaaaaaacc gtgttacctg
       61 ggctaacaac gaccagaaag cccattgtta caaaaaaact ttcagaaatc gttgccgaag
      121 agcttgaaag aatgatccgc caggaggaga taagggaagg tgaatacctg ccttctgaac
      181 gtgaacttat ggcattcttt ggcgttggaa gaccatctgt cagagaggct ttatcatctc
      241 tcagacataa agggctaatc cggtataaca agggagaaaa agccagtgtc tgccgtccat
      301 caccagaaac catcattaat gggttatcag gcattgttaa agatttttta tccacagctg
      361 atggcatcaa ggattttgaa agactacgca tcttttttga aaccaacctt gtaagatatg
      421 cagcaaaaaa tgcaacgcct gaggatattc acaggcttga gaaggtgctc agtatccatc
      481 ataactcact gagaaccccc gaagtgtatg ctcaatatga tattgaattt catagtatac
      541 ttgctgatat acctgacaat aaagtattaa aaacaataca ctcagcattt tctgaatggc
      601 tgataatggc caaacctctg gaaaagaata aaatgacata tgaaaataac aaaaaaaccc
      661 atcagcagca tattgaaata cttacggcca taaaagagca tgattgtgat aaagctgaac
      721 agctaatgta taatcacctt tataaacgaa ttgaagcatc atagttactc gacgcttcac
      781 caggtagtca ccataaatat tatttaaatc catgagaata catccagtcc ataaacagtt
      841 ttatccatcc tccaacagga agatctgtgg cccactgaac actaaagcca tgccctcctt
      901 tctcatagag atgcatttca gcagaaacgc ctttctctat taggccacgg tacatgagca
      961 gactgttttc aattgacaca agatcatcat cacaacaatg gagtaaaaac acaggaggaa
     1021 aacatgatgt gacattaaga tccgcactat attgtattat attttctgag gtactattgt
     1081 cccccagtaa ttgcttcctg gagtctggat gtgtcacatc atctttcatt gatattacag
     1141 gataaattaa cccgacaaaa tcgggaatag cgaaaatctc ttgttcttta ctgtcagaag
     1201 aacgtttgaa atcacaaatc tgattcacaa acgttgaggc gagatgcccc ccagcagaaa
     1261 atcctaatac accaatacga tgacgctgta gatgccattt atcagcattc tt
//
LOCUS       DAZGAN020000321         1301 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGAN020000321 DAZGAN020000000
VERSION     DAZGAN020000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.328"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..96
                     /locus_tag="ACHKYO_RS26435"
                     /old_locus_tag="pgaptmp_005287"
     CDS             <1..96
                     /locus_tag="ACHKYO_RS26435"
                     /old_locus_tag="pgaptmp_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4431917.1"
                     /translation="FNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            196..1068
                     /locus_tag="ACHKYO_RS26440"
                     /old_locus_tag="pgaptmp_005288"
     CDS             196..1068
                     /locus_tag="ACHKYO_RS26440"
                     /old_locus_tag="pgaptmp_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431918.1"
                     /translation="MNKISIDSDNRRIIYREMNVSRLLTGLKHVDVNNTIRRIYNMCP
                     EAHVTASNIIFNNHSYPDFYPLKQTICKDIIRNTLLSWFPIFSNKKCSSDMVLMFRDG
                     EYATLSSLLFFNEQAEEWLNKGPEYWEYSLLVKNEITSLWCKELHKLPELEVRTISSP
                     GLFLLKNMDIDVSSYAIYSNYLEKMKTRHEAVLRFFSRIILLARTLERNSQLHWNRFN
                     YIDWKVGVVETARGWLVHMAKISDEGIVIDYKIISPTTYFTSEDGLLSNTLKKCQGDL
                     LNKYIVLLAPGAEV"
     gene            1104..>1301
                     /locus_tag="ACHKYO_RS26445"
                     /old_locus_tag="pgaptmp_005289"
     CDS             1104..>1301
                     /locus_tag="ACHKYO_RS26445"
                     /old_locus_tag="pgaptmp_005289"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HGU4431919.1"
                     /translation="MAQCMIEQLENNSLIYGYNKVSFVLIEVGNIACIETESLMFCLD
                     IVSRGTLAENATFAVQKKRANA"
BASE COUNT          436 a          211 c          259 g          395 t
ORIGIN      
        1 tttaattata tcgaatgtga ttacaatcgg tggcggcggc acagttggtg tggcggcctc
       61 agtccggaac aatttgaaaa ccagaacctc gcttaggcct gtgtccatat tacgtgggta
      121 ggatcatact ggtgttgccc ggtttgccag acaacaaaag agggggttct taaactggac
      181 tcaaaggagt gaatattgaa taaaataagt atagacagcg ataatcgtcg tatcatctac
      241 agggagatga atgtttcaag gttattgaca ggccttaaac atgttgatgt aaacaatacc
      301 atcagacgga tttataatat gtgtccagaa gcacatgtta ctgcgtctaa tattatattt
      361 aacaatcatt cttaccctga tttttacccg cttaaacaga caatatgtaa ggacattata
      421 agaaatactc tgctttcatg gttcccaata ttcagcaaca aaaaatgctc atctgatatg
      481 gtgcttatgt ttagagatgg agaatatgca accctttctt cattactatt tttcaatgaa
      541 caagcagaag aatggctgaa taaaggtcct gaatactggg agtactcttt actggtaaag
      601 aatgaaataa ccagtttatg gtgcaaggaa ctccataaac tacctgaact tgaagtaaga
      661 accatctcca gtccgggatt atttttactg aaaaatatgg atattgatgt ttcgtcgtat
      721 gctatatact ctaactatct ggagaaaatg aaaacaaggc atgaagctgt tttacgtttc
      781 ttttcaagaa ttatattact ggcccgaacc ctggagagaa acagtcaact acactggaac
      841 agattcaatt atatagactg gaaagttggt gtagttgaaa cagcaagagg atggcttgta
      901 catatggcta aaatatctga tgagggaatc gtgattgact ataaaatcat atcgcctaca
      961 acgtatttca cttcggaaga tggtctttta tctaatacat taaaaaaatg ccaaggagac
     1021 ctcctgaata aatatatagt attacttgcc cctggtgctg aagtttaatt tatttaaaag
     1081 gtatttgtat acacgagtta tctattgcac aatgtatgat agagcaatta gaaaataact
     1141 cactaattta tggatataat aaagtcagtt ttgttttgat tgaggttgga aatattgcat
     1201 gtatagaaac tgaatcatta atgttctgtc ttgatattgt ttcaagagga acccttgcag
     1261 aaaatgcaac atttgctgtt cagaaaaaaa gagcgaatgc c
//
LOCUS       DAZGAN020000322         1289 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGAN020000322 DAZGAN020000000
VERSION     DAZGAN020000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.329"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(81..1232)
                     /locus_tag="ACHKYO_RS26450"
                     /old_locus_tag="pgaptmp_005290"
     CDS             complement(81..1232)
                     /locus_tag="ACHKYO_RS26450"
                     /old_locus_tag="pgaptmp_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU4431920.1"
                     /translation="MDKSLSRSVISVVIGGILFGVSAGVLAQDAAENKLIASGSIPEN
                     TDLSASARADGEIALAVGTGASADKEIATSVGSWSAARGQQSVALGGGTYAYADASTA
                     LGSIAFVDNTATYGTAAGNRAKVDKDATEGTALGAKATVTNKNSVALGANSRTTRDNE
                     VYIGYEAEPGKAYKTRVLGGLSDGTRPSDAATVRQVDRVKDSVEQLAQDTNTRLVVEA
                     KKSREYTDSRTTVGVNSDGTLTRAEGASKTLAVNDGLVALSGRTDRIDATVGSVDRRV
                     TKNTQAIQSTSRQLQEHNTRLNSHQRQINENHEEMKRAAAQSAALAGLFQPYSVGKFN
                     ATAAVGGYSDKQAVAVGVGYRFNEQTAAKAGVAASDGDVSWNAGVNFEF"
BASE COUNT          317 a          363 c          266 g          343 t
ORIGIN      
        1 cacctgtttc atgctcacat gagaaaaaac ggcagacaaa actccacgcc tgccgtcatg
       61 cttcatgtca ctgtcagcaa ttaaaactca aagttcacac cagcgttcca ggatacatcg
      121 ccatcgctcg ctgccacacc tgctttcgct gctgtctgct cattgaaacg gtaaccaaca
      181 cccacggcaa cagcctgttt gtcactgtaa ccaccaacgg cagcagtggc gttgaattta
      241 cccacactgt acggctggaa aagacctgcc agcgctgcgc tctgggctgc cgcacgtttc
      301 atctcttcgt ggttttcatt aatctgacgc tgatgactgt tcagacgtgt gttgtgctcc
      361 tgcaactgac gggaagtgga ctgaatagcc tgtgtatttt tagtaacccg gcggtcaaca
      421 ctaccaacag tagcatcaat acggtctgtt ctgccggaca aagctacaag cccatcatta
      481 acagccaggg ttttgctggc tccttctgca cgggtcaggg ttccgtcgga gttaacacca
      541 actgtcgttc tggagtctgt atattcacga gactttttcg cttcaaccac aaggcgggta
      601 ttcgtatcct gagccagttg ctcaacactg tcttttacac ggtcaacctg acgaacggtt
      661 gcagcatctg agggacgagt accgtcactc agaccaccaa gcacccgggt tttataagcc
      721 ttacccggct ctgcttcata accgatataa acctcattgt cacgggttgt gcgtgaatta
      781 gctcctaatg caacactgtt tttatttgtt actgttgcct ttgcccctaa cgcagtacct
      841 tctgttgcat ctttatcaac tttggctcgg ttacctgctg ccgtgccata tgtagccgtg
      901 ttatcaacaa atgcaatgct tcccagggct gtagatgcat ctgcataagc atatgttccc
      961 ccacctaaag ccacactttg ctgtcctcgt gctgcagacc atgatcccac tgatgttgca
     1021 atttctttat ccgcgctggc ccctgtacca acagctagcg caatctctcc atccgcgcga
     1081 gctgaagcag aaagatctgt attttctggg atactccctg aggcgataag cttattctcc
     1141 gctgcatcct gagccagaac gccagcgctt acaccaaata aaataccgcc aataacaacg
     1201 gaaataacac ttcgtgataa agacttatcc atgtatgatt cctcatgaat ttatttgtat
     1261 acaaacaaac cgaaatacat cggatgaat
//
LOCUS       DAZGAN020000323         1289 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGAN020000323 DAZGAN020000000
VERSION     DAZGAN020000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.330"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            71..>1289
                     /locus_tag="ACHKYO_RS26455"
                     /old_locus_tag="pgaptmp_005291"
     CDS             join(71..386,386..>1289)
                     /locus_tag="ACHKYO_RS26455"
                     /old_locus_tag="pgaptmp_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4431921.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEQLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRS
                     PESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQQASNGLSDNRCL"
BASE COUNT          329 a          307 c          381 g          272 t
ORIGIN      
        1 cagacaacca gtatcactta aataagtgat agtcttaata ctagttttta gactagtcat
       61 tggagaacag atgattgatg tcttagggcc ggagaaacgc agacggcgta ccacacagga
      121 aaagatcgca attgttcagc agagctttga accggggatg acggtctccc tcgttgcccg
      181 gcaacatggt gtagcagcca gccagttatt tctctggcgt aagcaatacc aggaaggaag
      241 tcttactgct gtcgccgccg gagaacaggt tgttcctgcc tctgaacttg ctgccgccat
      301 gaagcagatt aaagaactcc agcgcctgct cggcaagaaa acgatggaaa atgaactcct
      361 caaagaagcc gttgaatatg gacgggcaaa aaagtggata gcgcacgcgc ccttattgcc
      421 cggggatggg gagtaagctt agtcagccgt tgtctccggg tgtcgcgtgc gcagttgcac
      481 gtcattctca gacgaaccga tgactggatg gatggccgcc gcagtcgtca cactgatgat
      541 acggatgtgc ttctccgtat acaccatgtt atcggagagc tgcccacgta tggttatcgt
      601 cgggtatggg cgctgcttcg cagacaggca gaacttgatg gtatgcctgc gatcaatgcc
      661 aaacgtgttt accggatcat gcgccagaat gcgctgttgc ttgagcgaaa acctgctgta
      721 ccgccatcga aacgggcaca tacaggcaga gtggctgtga aagaaagtaa tcagcgatgg
      781 tgctctgacg ggttcgagtt ccgctgtgat aacggagaac aactgcgagt cacgttcgcg
      841 ctggactgct gtgaccgtga ggcactgcac tgggcggtca ctacgggcgg cttcaacagt
      901 gaaacagtac aggacgtcat gctgggagcg gtggaacgcc gcttcggcaa cgagcttccg
      961 gcgtctccag tagagtggct gacggataat ggttcatgct accgggctaa tgaaacacgg
     1021 cagtttgccc ggatgttggg gcttgaaccg aagagcacgg cggtgcggag tccggagagt
     1081 aacggcatag cagagagctt cgtgaaaacg ataaagcgtg actacatcag tgtcatgccc
     1141 aaaccagacg ggttaacggc agcaaagaac cttgcagagg cgttcgagca ttataacgaa
     1201 tggcatccgc atagtgcact gggttatcgc tcgccacggg aatatctacg gcagcaagcc
     1261 agtaatgggt taagtgataa caggtgtct
//
LOCUS       DAZGAN020000324         1275 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGAN020000324 DAZGAN020000000
VERSION     DAZGAN020000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.331"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..515)
                     /locus_tag="ACHKYO_RS26460"
                     /old_locus_tag="pgaptmp_005292"
     CDS             complement(<1..515)
                     /locus_tag="ACHKYO_RS26460"
                     /old_locus_tag="pgaptmp_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU4431922.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTP"
     gene            523..948
                     /gene="tnpA"
                     /locus_tag="ACHKYO_RS26465"
                     /old_locus_tag="pgaptmp_005293"
                     /pseudo
     CDS             523..948
                     /gene="tnpA"
                     /locus_tag="ACHKYO_RS26465"
                     /old_locus_tag="pgaptmp_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          343 a          311 c          274 g          347 t
ORIGIN      
        1 ggagttgata cttcactttc tgtctgaata ctgatgtacc acttaccgca ggactggctg
       61 acagtgacat ttttcacaac tcccgtgacc tgacggctgt tccggtagcg catccagccc
      121 agtttcggca gaaaaatacg gctgttttcc tgatcgagct taacgccctg cgggtagcgg
      181 aatacatcat tctgtccccg ctttttgaat cggggaaaag cagcccgctt ctggaagaag
      241 tttttgtaag cccgctcaag gtctttcagt gactgttgca atggctgtga cggggcatct
      301 ttaagccatt gcgtttcagt ggcatttttc cactcaacca gccaggaagc cattttcccg
      361 taagggatgt atttattacc gacctcatga ttctcattct gacgtgccag agcacgattg
      421 aaaacgaaac gacatgcgcc tgcgaagcgc ctcatttcac gctcctgttg accaccgggt
      481 cttaactgga atttaaatgc ctgtaatcgt ttcacaggcc aaatgatact ttggtctgtg
      541 aaaaaagaaa ccgatatccg gcgtggaagg cattgtgttt tcctgatgca tgttcacctg
      601 gtctttgtca ccagataccg acgtcagatt tttgaccatg acgcgacaga aaaactacgc
      661 acttactttt caaatgtatg tgctgatttt gaagctgaac tggttgaaat ggatggcgaa
      721 ccagatcacg tccatttgtt aattaactat cctcccaaac tggcgatatc cagtctggta
      781 aacagcctca aaggcgtatc gggtaggtga ctgcgacgag atcgaccaga tattgcagtc
      841 aggtattact acaaaggcgt tttgtggagt gctggctatt ttgccagtag ctgcggaggt
      901 gcgccaatat ccgtcatccg ccaatacatt gaacaacagc aaacacctgg tcaggtggaa
      961 aaccgcgcct tatatccctg ccctgaagga cggggtttta cggcgcaccg gataagcctg
     1021 gagattatct acaccagcat ggacatcatt gtactatgcc ccgtacatgc cccatcacat
     1081 cctcaacatc aaagtattag agccttgcaa accattatgc gtctcagttt gtctcatcta
     1141 tcttacgcaa aaaaaataga actaatatca agcggcgcta tattgcgtat taaataaaac
     1201 cacctcaatt acattaataa atccaaacaa acatcatgca aataatttcc atggttattt
     1261 ctaggctatt taaat
//
LOCUS       DAZGAN020000325         1271 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGAN020000325 DAZGAN020000000
VERSION     DAZGAN020000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.332"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(174..347)
                     /gene="gnsB"
                     /locus_tag="ACHKYO_RS26470"
                     /old_locus_tag="pgaptmp_005294"
     CDS             complement(174..347)
                     /gene="gnsB"
                     /locus_tag="ACHKYO_RS26470"
                     /old_locus_tag="pgaptmp_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGU4431923.1"
                     /translation="MNIENLKTKAEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLESYDVKINLI"
     gene            complement(519..749)
                     /gene="ynfN"
                     /locus_tag="ACHKYO_RS26475"
                     /old_locus_tag="pgaptmp_005295"
     CDS             complement(519..749)
                     /gene="ynfN"
                     /locus_tag="ACHKYO_RS26475"
                     /old_locus_tag="pgaptmp_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HGU4431924.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
     gene            complement(754..819)
                     /gene="ynfR"
                     /locus_tag="ACHKYO_RS26480"
                     /old_locus_tag="pgaptmp_005296"
     CDS             complement(754..819)
                     /gene="ynfR"
                     /locus_tag="ACHKYO_RS26480"
                     /old_locus_tag="pgaptmp_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HGU4431925.1"
                     /translation="MKAPSGAFWLGVYSMDTHILR"
     gene            complement(822..1010)
                     /gene="ynfQ"
                     /locus_tag="ACHKYO_RS26485"
                     /old_locus_tag="pgaptmp_005297"
     CDS             complement(822..1010)
                     /gene="ynfQ"
                     /locus_tag="ACHKYO_RS26485"
                     /old_locus_tag="pgaptmp_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU4431926.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(1021..1233)
                     /gene="cspI"
                     /locus_tag="ACHKYO_RS26490"
                     /old_locus_tag="pgaptmp_005298"
     CDS             complement(1021..1233)
                     /gene="cspI"
                     /locus_tag="ACHKYO_RS26490"
                     /old_locus_tag="pgaptmp_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HGU4431927.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          400 a          256 c          206 g          409 t
ORIGIN      
        1 tacacagatc ttaccacgct aaaccataac gtccggcttc tctcactcct gagccggact
       61 gcattggttt aataaaaacc atcaacaatt gtgatttaga tattcggaac cattcaaata
      121 taacaaaacc ccgtaaaaac gaggtttatg gataaatttt attattgaat acatcagatt
      181 aaattaatct tgacatcata gctttcaaga cccgtcattt tttcccgtgc ggtaaactga
      241 atactggtaa cttctttccc ggtctttttc ttaagttcaa taattttttt tgttatatat
      301 tcagaaatat ctgcttctgc ttttgttttt aagttttcaa tattcatcat ttcctctttt
      361 agtctgttat gactttccag ttacacagta agtcgattat atggtgcaaa cgtgtaaaag
      421 ataagatgaa acatcgcaat aatcaacata cgatagtcta aattttacac aaacagacaa
      481 agagaatttt cctgaattat caatgcaata gcatcaaatc aactcaagag ccttattgct
      541 gcttccagaa tttcttctga agtaacatgt cgatccgcgg ctacataaat gactttatga
      601 tctccggtca gagatggaaa ccctgcggcc attacagtaa ggtgtgtttt ttcgccattt
      661 ggatattcac gcatgatggt gttaactcca gtcatcgctg gcactaccac tgctggttca
      721 gagttaaaaa aaactatgat ttttttcatg atgttaccgt agtatgtgag tatccatcga
      781 atagacacca agccaaaaag ctcccgaagg agccttcatt ttcacttttt taaatccaac
      841 gacagacggc tagcatttaa gtattgtgaa atattatcaa atgtaatcat cattgattta
      901 caaaagatac attttgcccc gaaaggattc atgtcagaaa catcaaaaga tgatgttcta
      961 tactgggaac catgacaaca cgggcatcta aagtgaatat ggtttgtaat attgtctacc
     1021 tcaaagcgcc actacatgaa cagcggcagg acctttaggt ccgttctcaa taccaaattc
     1081 aacttcctga ttctcagtta atgttttgaa atcgttgctc tgaattgctg agaaatggac
     1141 aaacacatct ttgctgccat ctttcggcgt gatgaaacca aaaccttttt cagggttaaa
     1201 ccattttact aaaccagtca ttttgttaga cataattatt accttttgaa gaaattagcc
     1261 cttgggcaga a
//
LOCUS       DAZGAN020000326         1262 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGAN020000326 DAZGAN020000000
VERSION     DAZGAN020000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.333"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..559
                     /locus_tag="ACHKYO_RS26495"
                     /old_locus_tag="pgaptmp_005299"
                     /pseudo
     CDS             1..559
                     /locus_tag="ACHKYO_RS26495"
                     /old_locus_tag="pgaptmp_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            710..1219
                     /locus_tag="ACHKYO_RS26500"
                     /old_locus_tag="pgaptmp_005300"
     CDS             710..1219
                     /locus_tag="ACHKYO_RS26500"
                     /old_locus_tag="pgaptmp_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HGU4431928.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          346 a          297 c          279 g          340 t
ORIGIN      
        1 tattcctgtt cacgatacta ttgccagagt tgtatcctgt atcagtcctg caaaatttca
       61 cgagtgcttt attaactgga tgcgtgattg ccattcatca aatgataaag acgtcattgc
      121 aattgatgga aaaacgctcc ggcactctta tgacaagagt cgccgcaggg gagcgattca
      181 tgtcattagt gcgttctcaa caatgcacag tctggtcatc gggcagatca agacggataa
      241 gaaatccaat gagatcacag ctatccctga acttcttaac atgctggata ttaaaggaaa
      301 aatcatcaca accgatgcga tgggttgcca gaaagatatt gcagagaaga tacaaaaaca
      361 gggaggtgat tatttattcg ctgtaaaagg aaaccagggg cggctaaata aagccttcga
      421 ggaaaaattt ccgctgaaag aattaaataa tcccaagcat gacagttacg caattagtga
      481 aaagagtcac ggcagagaag aaacccgtct tcatattgtt tgcgatgtcc ctgatgagct
      541 tattgatttc acgtttgaat agaaagggca gctatggtta gaaactacct gacgtcagtc
      601 cttgcgggga gcaggctttc gtaaatttgt cctgctacaa caggattaac ttcacaaata
      661 tcatttctca acgtctacac ttactcctgt aaaccgttca ggagcagtaa tgaatcagca
      721 acgatttgat gacagcaccc taatccgtat cttcgccctt catgagttac atcgactgaa
      781 agaacatggc ttaacgcgcg gggcgcttct cgattatcac agccgctata aactcgtctt
      841 tctggcgcat tctcagccgg agtaccgcaa acttggcccg ttcgtggctg atattcacca
      901 gtggcaaaat ctggatgatt tttacaacca gtactaccaa cgcgtaattg ttttgctttc
      961 tcatcccgcc aacccgcgcg atcacaccaa cgttttgatg cacgttcagg gttattttcg
     1021 cccgcatatt gattccacag aacgccagca gttggcggcg cttatcgaca gttatcgccg
     1081 tggcgagcaa ccacttcttg cgccgctgat gcgtatcaaa cactatatgg cgctttatcc
     1141 tgacgcctgg ctttcagggc agcgttattt cgaactttgg ccgcgtgtga ttaacttgcg
     1201 ccattcagga gttttatgat tacccatctg gtctggtttc gccaggattt acgtctgcac
     1261 ga
//
LOCUS       DAZGAN020000327         1253 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGAN020000327 DAZGAN020000000
VERSION     DAZGAN020000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.334"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..332)
                     /locus_tag="ACHKYO_RS26505"
                     /old_locus_tag="pgaptmp_005301"
     CDS             complement(<1..332)
                     /locus_tag="ACHKYO_RS26505"
                     /old_locus_tag="pgaptmp_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431929.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAVLTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNPQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAG"
     gene            complement(335..991)
                     /locus_tag="ACHKYO_RS26510"
                     /old_locus_tag="pgaptmp_005302"
     CDS             complement(335..991)
                     /locus_tag="ACHKYO_RS26510"
                     /old_locus_tag="pgaptmp_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431930.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELASNLTRSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAVLGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYVMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            complement(1086..>1253)
                     /locus_tag="ACHKYO_RS26515"
                     /old_locus_tag="pgaptmp_005303"
     CDS             complement(1086..>1253)
                     /locus_tag="ACHKYO_RS26515"
                     /old_locus_tag="pgaptmp_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431931.1"
                     /translation="VERYLPEVKTLTRLAGGRWAEFHTARRGFIRLGKRLGFERMPDD
                     EDGFMVFRIAV"
BASE COUNT          263 a          386 c          285 g          319 t
ORIGIN      
        1 cctgctattg ccagggcatt acgtaacccc agcgtattac tgtcgggatt atccgggcgg
       61 cttaccccgt atctcgccat ctggttatcc tgcgccatct gcgctgtacg gagactggaa
      121 gtggcaaggc ctcccacccg tgcaagctgt ggattcatca gactgttgtt ctccccaagg
      181 tcagccagcc ttgctacacg gggtaaatat ctggtgcgcc agtcgtcgta ttgctggcgg
      241 gtcagcactg cggatgtctg ccagtccccc tgagggcggg ctgctccggt ataaccgtat
      301 ctcgcaagtg tttcgtattt accgtactcc ataatcacag tctccagttc tgagcctgat
      361 gctgaatggc attagcgcca gtgccaggtg ttttagcacc gccacttcct ccgctaccgc
      421 cagccttatg catcacatat gcacctgccg cacccagacc agcgccaaca agagaggctc
      481 gcccctgctg tttcgtaaac gccgcctgtg catccgattt agcttttgcc agactgctgt
      541 ctgccagaga gttaaaactc tgtaacgcat ccgccttctg accagaaccg agaacagcaa
      601 catcctgtag cccggcaaca tacttatctg cctgcgatac ctgcccccgt gtggttgtgt
      661 caatctgccc ggttacctga tcactctggt tcgcattcat taccgcgtta aagcgaccac
      721 tggacgggtc aacaccggac cgagtgagat tacttgccag ctccttccgc gcttcgccaa
      781 actgtttttg ataccccaga tttgtggtgc cagcaatatt gtcgtactgc tgctcactgt
      841 tcaggtcatc gaccttttcc atgaagttat cttcagccgg gcggaggata tttttgtaat
      901 cctgccaccc tttccaggcc acttcttcct gtgctatttg cgctgctgtt ggttttactt
      961 tggtatcacc accaccttta cttccaccca taatggcccc ctggataata aaaaaaccct
     1021 gccggagcag ggtcagaatg tgaattgcaa tgttggttta aacacgatga tgaaaactgt
     1081 ggcagtcaga ccgctatcct gaacaccatg aagccatcct catcatccgg cattcgctca
     1141 aagcccagac gttttcccag ccggataaat ccccgccttg ccgtatgaaa ttcagcccaa
     1201 cgtccgccag ccagacgggt taatgtcttc acctccggca gataacgctc aac
//
LOCUS       DAZGAN020000328         1248 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGAN020000328 DAZGAN020000000
VERSION     DAZGAN020000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.335"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..344
                     /locus_tag="ACHKYO_RS26520"
                     /old_locus_tag="pgaptmp_005304"
     CDS             <1..344
                     /locus_tag="ACHKYO_RS26520"
                     /old_locus_tag="pgaptmp_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431932.1"
                     /translation="PSGGTAVAANRLATPRNINGVPFDGTLDINITSGMTQSTADGRY
                     VQNVQLGAQSYHSPGGNEMSWNYSAPSGCMLSGINVQETGSRSADNIGGVYYRPVQIY
                     INNAWRTVSSV"
     gene            complement(379..900)
                     /locus_tag="ACHKYO_RS26525"
                     /old_locus_tag="pgaptmp_005305"
     CDS             complement(379..900)
                     /locus_tag="ACHKYO_RS26525"
                     /old_locus_tag="pgaptmp_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4431933.1"
                     /translation="MQLINVKRYYPKDKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            complement(1022..>1248)
                     /locus_tag="ACHKYO_RS26530"
                     /old_locus_tag="pgaptmp_005306"
     CDS             complement(1022..>1248)
                     /locus_tag="ACHKYO_RS26530"
                     /old_locus_tag="pgaptmp_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431934.1"
                     /translation="MPESTANQRYVTGVRLGAQALSGGLEYNYSLSSGNVITGFKTDG
                     DWEMRGGDDRVYYRQIQYCINGHWVSAASI"
BASE COUNT          341 a          312 c          256 g          339 t
ORIGIN      
        1 tgccatcggg tggaaccgcc gtggcagcaa acagactcgc caccccaaga aatattaatg
       61 gtgttccttt tgacggaacg ctggatatca acatcacgtc aggaatgacg cagtcaaccg
      121 cagatggccg gtatgtacag aatgttcagc ttggagcaca gagctatcat tcacccggag
      181 gcaatgaaat gtcatggaat tacagcgcac cttccggttg tatgctttcc ggaattaacg
      241 tgcaggaaac cggctcccgg tctgcggaca atatcggtgg ggtctattat cgcccggttc
      301 agatttatat taataatgcc tggagaacgg taagttcagt gtaaaccaca gaaaagggtg
      361 ctgaatgcac cctctgaatt attccggttt ttccggccag ggaatcgcct ggtaatgccc
      421 ctcactggca atatcattaa agtgcatttc tttaagctgt cggatatacg ccatccagcg
      481 cgtcagctgt aatttatttt catcgctaat catgtccagt tttaattctg ttttccagtc
      541 atccacggtt tcgttggctt cctggagcag cgatgagcga cgcttttcag caaccgtctt
      601 ccagtttacc ggcacctttg atatcaggcc atcagaaaac tgccagttac cgtcaatatt
      661 cacaccttcc ggcagttcgt ccacttcaac aacagtaaaa cctgccggat ataaagcaga
      721 gacatcttca gcaacagagc agataatacc cgttacaggc gaaatacaga gtttgtattt
      781 ctttgtaaaa agcgggatag attcataaaa atcccttccg tcctcactct ggaaatactg
      841 aacatcctca ccatagggtt tgtctttcgg gtaatatcgt ttgacattaa tcagttgcat
      901 aaacatcacc atgtaaatca atgataaaaa caggaataat actatcgcca tatacaggca
      961 atgcttgtct gaaataacgc atatttcaga tgctggctgc tgtgtgctat gcccccgtta
     1021 attaaatact tgcagcggat acccaatgcc cattaataca atattgtatc tgtctgtaat
     1081 aaacacggtc atcaccacca cgcatttccc agtctccgtc agtcttaaaa ccggtaatca
     1141 catttcctga tgacagggag taattatatt ccagtccgcc gcttaatgcc tgcgctccca
     1201 gtcttacccc tgtaacataa cgctgattgg ccgttgattc cggcattc
//
LOCUS       DAZGAN020000329         1247 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGAN020000329 DAZGAN020000000
VERSION     DAZGAN020000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.336"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..869)
                     /locus_tag="ACHKYO_RS26535"
                     /old_locus_tag="pgaptmp_005307"
     CDS             complement(<1..869)
                     /locus_tag="ACHKYO_RS26535"
                     /old_locus_tag="pgaptmp_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HGU4431935.1"
                     /translation="MKKASKLLLALMPALSLSLFSAGAYASGYSVTPETLNSNSMTSA
                     EFLKGIGVYPKEGGDPLIKLVDGKVTPLKGNTPTSLNIDAVSGKYLKYTLIDYGKAGL
                     TDTELVAPRGDIVSYYDYANETAYFVNTKTNQILFSADGEGVVSTVGADGKTYSRHIP
                     EISEYIYEIKMKAVQYGATIDTGLSAADVTDIHARLSSLGTGKTQVGGGATAGGENAT
                     AVGYNATANHTNSVAVGANSQTTRPDEVNVGNRVIGGLRDGVQAADAATYGQLERYRI
                     AEENARIQGDAQTL"
BASE COUNT          375 a          292 c          225 g          355 t
ORIGIN      
        1 agtgtctgtg catcaccctg aatacgggca ttctcttctg caatacgata gcgctctaac
       61 tgaccatagg ttgcggcatc cgcagcctga acaccatcac gaagcccacc aattacacga
      121 ttccccacat ttacttcatc ggggcgagtg gtctgagagt tagcccctac agcaacactg
      181 ttcgtatggt ttgcagtggc gttgtaaccg acagcagtgg cgttttctcc gcctgctgtt
      241 gccccgccac caacctgagt tttaccggtg cccagagaag acagacgggc gtgaatatct
      301 gttacatctg cagcagaaag accagtgtca atagtggctc catattgcac agccttcatt
      361 ttaatttcat aaatatattc actgatttca ggaatatgtc ttgaatatgt ttttccatca
      421 gcgcctactg tcgagacaac tccttcgcca tctgcactaa acagaatttg attggttttt
      481 gtgtttacaa aataagctgt ctcattcgcg taatcataat atgagacgat gtctcccctt
      541 ggagcaacta actcggtatc tgtcaggcca gccttaccat aatcaattaa ggtatatttc
      601 aaatatttac cggaaacagc gtctatattt aatgaggttg gtgtattccc tttaagagga
      661 gtaaccttac catcaacaag cttaattaaa ggatcccctc cctcttttgg atatacaccg
      721 atacctttca aaaattccgc agaagtcatt gaattactat tcagagtttc aggtgtaacg
      781 gaataaccac tggcatacgc tccagcagaa aataatgaca ggcttaatgc tggcattaaa
      841 gccaacaata gcttgcttgc ctttttcatg atgtgtaccc ttatgttaaa tcatgttata
      901 agtatgttga cattcggaac gcaaatgtct tcagggatac tactcacaat aacaaggaaa
      961 ataaaatatt aaaatcaatg agttaagaaa aattaataat taaaatcaca aggatgcaat
     1021 acgcatcaaa aacttaccct aaataacacc agtattactc agaaaaaaca aaacattttc
     1081 tgtagttatt atttaccatt gataaatatt acctctctct gtcagcctgc attgtataaa
     1141 acatacacta caaacgggtg ccatatcggc tgaacctcag caaccagttg acggggactt
     1201 tcgtccccgt cgcggttttc ctactgctta cactgtaaga acgccgc
//
LOCUS       DAZGAN020000330         1245 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGAN020000330 DAZGAN020000000
VERSION     DAZGAN020000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.337"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1245
                     /gene="istA"
                     /locus_tag="ACHKYO_RS26540"
                     /old_locus_tag="pgaptmp_005308"
     CDS             <1..>1245
                     /gene="istA"
                     /locus_tag="ACHKYO_RS26540"
                     /old_locus_tag="pgaptmp_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HGU4431936.1"
                     /translation="IKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNK
                     CVTLQLLWEEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPI
                     VGVTDPETGEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELV
                     VPDNLKSATSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERW
                     IMARIRHEIFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEAS
                     YSYTLVKKVRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPR
                     KRTYGYSTRNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYR
                     FCLGLLNLHKKYSKARLNAACA"
BASE COUNT          340 a          307 c          306 g          292 t
ORIGIN      
        1 cataaagcag tggcccttac ctacaggatg ggatgataca aaactaaaac atgcgttcct
       61 tcagacccag gttaagatga agaagcactc tctgcctgac tgggctacag tacaccggga
      121 actgcgtaat aaatgcgtga cgctgcagct actctgggaa gaatactgcg agcgtaatcc
      181 aggcggtttt tacagctata accattactg ccggatgtac cgtgaatggc tcaaaaccac
      241 ttcaccatca atgcgtcagg tacataaagc tggcgaaaaa cttttcgttg attactgtgg
      301 acctatcgtt ggcgttaccg accctgagac cggagaaata agaactgctc aggtcatcgt
      361 agctgttctc ggggcatcaa gttacacatg ggcagaggcc acctggtctc agcagcttga
      421 agactgggtg atgagtcatg ttcgctgctt ccagtggttg ggtggcgttc ctgaacttgt
      481 tgttccggac aatctgaaaa gcgccacatc cagggcatgt aagtatgatc ctgacgttaa
      541 ccctacctac cagcagatgc ttgagcatta taatgtcgca gttttgcctg cgcggccacg
      601 taaaccgaaa gataaagcca aagctgaagt tggcgttcag gttgttgaac gctggatcat
      661 ggcccgaatc aggcatgaga tcttctacag ccttgcatcg cttaatcagc gcattcggga
      721 gttgctggaa agactgaata acaaaataat gcagaagttg ggttattcac gtgcagaact
      781 cttcatccag cttgataaac ccgcactgaa gcctcttcct gaagccagtt acagttacac
      841 cctggtgaag aaagtcagag ttcatgccga ttaccacgtg gaaatcgaca aacattacta
      901 ctcggttcca tgttcgctgt taggccagca actggaagca tggatctccg gagaactggt
      961 aagactcttc aatcaggggc aggaggttgc tgtgcacccg cgcaagcgta cttatggcta
     1021 cagtacccgc aacgagcaca tgcctgaagc tcatcgacag catgccacct ggacgccaga
     1081 gcgtcttctg gaatgggcgg ggcacatagg cagtgaaact catagttatg tgcttcatat
     1141 actgaactct cgtccacatc cggaacaaag ctatcgcttc tgccttggac tcctgaacct
     1201 tcataaaaaa tacagtaaag ccagacttaa tgcagcatgt gcaag
//
LOCUS       DAZGAN020000331         1230 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGAN020000331 DAZGAN020000000
VERSION     DAZGAN020000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.338"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..269
                     /locus_tag="ACHKYO_RS26545"
                     /old_locus_tag="pgaptmp_005309"
     CDS             <1..269
                     /locus_tag="ACHKYO_RS26545"
                     /old_locus_tag="pgaptmp_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431937.1"
                     /translation="VPFDGTQDINITSGMTESTANQRYVTGVRLGAQALSGGLEYNYS
                     LSSGNVITGFKTDGDWEMRGGDDRVYYRQIQYCINGNWVSAASV"
     gene            390..911
                     /locus_tag="ACHKYO_RS26550"
                     /old_locus_tag="pgaptmp_005310"
     CDS             390..911
                     /locus_tag="ACHKYO_RS26550"
                     /old_locus_tag="pgaptmp_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4431938.1"
                     /translation="MQLINVKRYYPENTLYGNDVQYFQSEDGKDFYESLPLFTKKYKL
                     CITPDSGIICSISQDASALYPAGFSVVEVDELPEGADISGNWKFDNGVISRIPVNYAR
                     KLEAMRQLYLNQAYEKINDWRTELQLGTISDEDRAALTRWMAYISQVKKMEPSAIKTE
                     AEFDAIKWPEQPE"
     gene            complement(947..>1230)
                     /locus_tag="ACHKYO_RS26555"
                     /old_locus_tag="pgaptmp_005311"
     CDS             complement(947..>1230)
                     /locus_tag="ACHKYO_RS26555"
                     /old_locus_tag="pgaptmp_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431939.1"
                     /translation="VPFDGTQDISITSGMTQSTADDRYVQNVQLGAQSYHSPGGNEMS
                     WNYSAPSGCMLSGINVQETGKNSADNIGGVYFRPVQIYISNAWRTVSSV"
BASE COUNT          352 a          262 c          284 g          332 t
ORIGIN      
        1 atgttccttt cgacgggacg caggatatca acattacttc agggatgacg gaatcaacag
       61 ccaatcagcg ttatgttaca ggggtaagac tgggagcgca ggcattaagc ggcggactgg
      121 aatataatta ctccctgtca tcaggaaatg tgattaccgg ttttaagact gacggagact
      181 gggaaatgcg tgggggtgat gaccgtgttt attacagaca gatacaatat tgtattaatg
      241 ggaattgggt atccgctgca agtgtttaat taacggctca tggcgtacgg caggcagcgc
      301 ctgatatatg cgttatctca gacaacccct gtccgtatat agcgatagta ttattcctgt
      361 ttttatcatt gattcacatg gtgatgttta tgcaactgat taatgttaaa cgatattacc
      421 cggaaaacac cctctatggt aatgatgtac agtattttca gagtgaagat ggtaaggatt
      481 tctacgagtc cctgccatta ttcaccaaaa aatataaact ttgtataaca cctgacagcg
      541 gcattatctg ctcaatatca caggacgctt cggcgctgta tcctgcgggg ttctctgtgg
      601 ttgaagtcga cgaactccct gaaggtgctg atatttcggg taactggaag tttgataatg
      661 gcgtgatctc ccgtattccg gttaactacg caagaaaact ggaagcaatg cgccagttat
      721 atcttaatca ggcatatgaa aaaattaatg actggagaac agaattacag ctgggcacca
      781 tcagtgatga agacagagcc gcacttaccc gatggatggc atacatcagt caggtgaaaa
      841 aaatggaacc atccgccatt aaaactgaag ctgaatttga tgccattaaa tggcccgaac
      901 agcctgaata acttaaaggg tgcttatcgc accctttttt ctttcatcag acagatgaaa
      961 ctgtacgcca tgcattactg atatatatct gcacaggacg gaaataaacc ccgccaatgt
     1021 tatcggcaga gttttttcct gtttcctgca cgttaatacc ggaaagcata caaccggaag
     1081 gcgcactgta attccatgac atttcattgc ctccgggtga atgatagctc tgtgctccaa
     1141 gctgaacgtt ctgtacatac cggtcatctg cggttgactg cgtcatccct gaagtaatgc
     1201 tgatatcctg cgtcccgtcg aaaggaacac
//
LOCUS       DAZGAN020000332         1224 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGAN020000332 DAZGAN020000000
VERSION     DAZGAN020000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.339"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..174
                     /locus_tag="ACHKYO_RS26560"
                     /old_locus_tag="pgaptmp_005312"
     CDS             <1..174
                     /locus_tag="ACHKYO_RS26560"
                     /old_locus_tag="pgaptmp_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HGU4431940.1"
                     /translation="NNDVPVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLT
                     SADEILAYRVDWK"
     gene            667..>1224
                     /locus_tag="ACHKYO_RS26565"
                     /old_locus_tag="pgaptmp_005313"
     CDS             667..>1224
                     /locus_tag="ACHKYO_RS26565"
                     /old_locus_tag="pgaptmp_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431941.1"
                     /translation="MSKKFSKTILSSAVAGLMLVSSGAMAAEPEVINVGDGTQYQVKL
                     YKNGEVAEVYDVKAGRQFLGAINTTTGSVSTVNNAEIKSAMELFLKENPSAVGQRQAV
                     AQIIGEMHRYSINVPSFKLDNIDTLTVTDIESIKANVDAVSKVITSKTAADYNQAVSN
                     GMSSEAALTVAKDGGAVLKEFNRIDS"
BASE COUNT          399 a          210 c          303 g          312 t
ORIGIN      
        1 aacaatgatg tgccggtgac agcagaagag cttatagcac tgagtgaagc ggcagagcag
       61 gcgatgttca ccaaagggat ggaaatccac gtccggcagc gcaccatgaa gaaggagctg
      121 gaaaagctga ccagtgcgga tgagatactg gcatacaggg tggactggaa ataaaaagaa
      181 ggggagcgga tgcacccctg atatctgagt agcatgctgt ttatagttat cattcattaa
      241 aagaatatta ccgtatggca cggcagcaga acatgaaatt tgtctcagaa ctgcaaatca
      301 gcattacggg tagtcaggca gataacacaa cgggataaaa aaacagcaaa atgcattaat
      361 aaaaaatttt tacaggttgg ttgccgggcg ttatgtgacg catatcaaaa acagaactgt
      421 aacgggagat aacaaaatga tggattctgg cggggagtaa cagcgcaatg cgttccggga
      481 tgctgtttct gagaatgtgg ctgaaaacgc ccatgctata gtaagaaaaa tcctaatagt
      541 tttatattaa tttttacgga actattcgga atacccttat gaaaggcaac gaagtgtcag
      601 tatttttaat gtattctgac acgcgttgct ctgtttgtat acaaatcatt tcatagggta
      661 taccagatga gcaaaaaatt ctctaagaca attctttctt ctgctgttgc cggactgatg
      721 ctggtgagtt ctggggctat ggctgcggag cctgaagtta ttaatgtcgg tgatggaacg
      781 caatatcaag ttaagttgta taagaatggt gaggttgctg aagtatatga tgttaaagca
      841 ggtagacagt ttttgggagc gataaataca actacaggaa gtgtaagcac agtaaataat
      901 gcggaaatca aatctgcgat ggagcttttt ctgaaggaaa atccatcggc agtgggacag
      961 aggcaagctg ttgcacaaat aattggagaa atgcatcggt atagcattaa tgtcccttct
     1021 ttcaagttag ataacattga tactcttacc gtaactgata ttgaaagcat caaagcaaac
     1081 gttgatgcag tttccaaagt tattacctcc aaaaccgcag ccgattataa ccaggctgtc
     1141 agcaatggta tgagttccga agctgcactg actgtagcta aagatggtgg tgcagtctta
     1201 aaagaattca accgaattga ttcg
//
LOCUS       DAZGAN020000333         1218 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGAN020000333 DAZGAN020000000
VERSION     DAZGAN020000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.340"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1072
                     /locus_tag="ACHKYO_RS26570"
                     /old_locus_tag="pgaptmp_005314"
     CDS             <1..1072
                     /locus_tag="ACHKYO_RS26570"
                     /old_locus_tag="pgaptmp_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4431942.1"
                     /translation="PNPVEAVSPLPSPVSAPSPAPEASVTDVLTGENAGESGEYRDET
                     RWLLTGYRSTVNSSAVRDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIM
                     SGTGSAGGGFSDNYTHVQVGADKKHELDGLDLFTGVTMTYTDSNAGSDTFSGKTKSVG
                     AGLYASALFDSGAYIDLIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVT
                     EDAWIEPQAELVYGAVSGKQFSWKDHGMSLSMKDKDYNPLIGRTGVDVGKSFSGKDWK
                     VTARAGLGYQFDLLANGETVLRDASGEKRIKGGKDGRMLMSVGLNAEVRDNIRFGLEF
                     EKSAFGKYNVDNAVNASFRYSF"
BASE COUNT          286 a          281 c          373 g          278 t
ORIGIN      
        1 gccgaatcct gtggaagctg tatcaccgct cccttcaccg gtttctgctc cgtcaccggc
       61 tccggaggcg tcagtgacgg atgtactgac cggagagaat gcgggggaat caggtgagta
      121 ccgtgatgag acccggtggc tgctgacagg ataccggagt acggttaata gctccgcggt
      181 cagggatgcg gggatgctga tgtcaatggg gcaccgtaac ttcattaatg aggtcaacaa
      241 cctgaacaaa cgtatgggtg acctgcggga catcaacggc gaggccggtg catgggcccg
      301 tatcatgagc ggaaccggct ctgccggcgg tggcttcagt gacaactaca cacacgttca
      361 ggtgggtgcg gataaaaaac acgaactgga cggtctggac ctcttcaccg gggtgaccat
      421 gacatatacc gacagcaacg ccggcagcga caccttcagt ggtaaaacga agtcagtggg
      481 tgcgggtctg tatgcttcag ccctgtttga ttccggggcc tatatcgacc tgattggtaa
      541 gtatgttcac catgataatg agtatacggc gtcctttgcc ggactcggca caaaagacta
      601 cagctctcat tcctggtatg ccggtgctga agtgggttac cgctatcatg tcacagagga
      661 tgcctggatt gagccacagg cggaactggt ctatggtgct gtatccggta aacagttcag
      721 ctggaaggac cacgggatga gcctgtccat gaaggacaag gactacaatc cgctgattgg
      781 ccgtaccggt gtggatgtgg gtaaatcgtt ctccggtaag gactggaaag tgacggcacg
      841 tgctggtctt ggctatcagt ttgacctgct ggcgaacgga gaaacggtac tgcgtgatgc
      901 atccggagag aagcgtatta agggcgggaa agacggtcgc atgctgatgt ctgtgggtct
      961 gaacgcagag gttcgtgaca acatccgttt tggtctggag tttgagaaat cagcctttgg
     1021 taagtacaac gttgataatg ctgtcaacgc cagcttccgt tattcattct gacggttgtt
     1081 actgaagggg attatggcct gacggctccc tgtaaataaa gacaggcaca gaagattgtg
     1141 agttacttag tgttccacca aaaagtaagg ccgcaactct gtgcctgctt cgtaagtgta
     1201 cagtgagggc catatggt
//
LOCUS       DAZGAN020000334         1193 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZGAN020000334 DAZGAN020000000
VERSION     DAZGAN020000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.341"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            68..433
                     /locus_tag="ACHKYO_RS26575"
                     /old_locus_tag="pgaptmp_005315"
     CDS             68..433
                     /locus_tag="ACHKYO_RS26575"
                     /old_locus_tag="pgaptmp_005315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431943.1"
                     /translation="MSRSAKPRKRKPAPQRSKLPRYVVKLHDDDFFDEEDAEALRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVPPPF"
     gene            433..>1193
                     /locus_tag="ACHKYO_RS26580"
                     /old_locus_tag="pgaptmp_005316"
     CDS             433..>1193
                     /locus_tag="ACHKYO_RS26580"
                     /old_locus_tag="pgaptmp_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HGU4431944.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCSHIKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVIQEICLQADVVPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTPDHTWKNLHFYARKVMSMWRYRITRLLSRKYPELVIPDALAVEGSSRR
                     DWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYL"
BASE COUNT          268 a          311 c          335 g          279 t
ORIGIN      
        1 gcggcttgtc atggcctgaa attaccgtta taaaaacaga caatatcatt gtctttcagg
       61 tagttatatg tcccgttcag ctaaaccccg taaacgaaaa cctgcccctc aaagaagcaa
      121 acttccccgc tatgtcgtga agcttcacga cgatgacttc tttgacgaag aagacgcaga
      181 agctctgcgc tttgataatt ttgacgatgc cgttgagtgc tgcgcagacc tgaatattcc
      241 cttctttgtg gatgccggaa acaaaaagct ggtcttctgg tttgttcgtg ttgatgacga
      301 agggtatcct gaaatagccc gctgcacgga gcgggagttt gcgaccattc ttgccggtat
      361 cagcgccggc ggcatgtact gcccggagtg tggcacggtt cactggccgg acggagtccc
      421 cccgcccttc tgatgcttcc ccgttttgcc gacatttttc agcagggaaa ccgctggctt
      481 aactggctgg agaaacaacc ggaaggttca gtgcgtccgg tagtcattga gtctgtgaca
      541 aaaatcatgg cctgcgggac cacgctgatg gggtacacac agtggtgctg ttcatctccg
      601 gactgcagcc acataaaaaa ggtctgcttc cggtgtaaaa gtcgctcctg cccgcactgc
      661 ggagtgaagg ctggcgcaca gtggatacag tatctgctga gtctggttcc cgactgtccg
      721 tggcagcata ttgtgttcac acttccctgc cagtactggt ccctggtgtt ccacaaccgg
      781 tggttactgg cagagatgag ccgcattgct gcggatgtga tacaggaaat ctgcctccag
      841 gcagatgtgg tgccggggat attcacggtg atccacacat ggggacgtga ccagcagtgg
      901 catccgcaca ttcacctgtc gacaacggcc ggcggcgtga caccagacca cacctggaaa
      961 aaccttcatt tttacgcccg taaggtgatg agcatgtggc gttaccggat aacacggttg
     1021 ctgtcacgga aatacccgga gctggtgata ccggatgcgc tggcagttga aggaagcagc
     1081 agacgggact ggaatcgctt cctggacagt cattaccggc ggggctggaa tgtcaacgta
     1141 tcccgggtga tggataacgc cacacatgtg gcggtatact tcggctctta cct
//
LOCUS       DAZGAN020000335         1173 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGAN020000335 DAZGAN020000000
VERSION     DAZGAN020000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.342"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            61..>1173
                     /locus_tag="ACHKYO_RS26585"
                     /old_locus_tag="pgaptmp_005317"
     CDS             61..>1173
                     /locus_tag="ACHKYO_RS26585"
                     /old_locus_tag="pgaptmp_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU4431945.1"
                     /translation="MKKMTVALSAVAVAVMFAAGAQAAEVYNKDGNKLDLYGRATALH
                     YFSDDKGNDGDQTYARLGFKGETQINDQLTGFGQWEYQFSGNKTESEGSAGNKTRLAF
                     AGLRFADVGTIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNT
                     DFFGLVDGLNFAAQYQGKNERDDLQKANGDGYGFSASYEFDGFGFVAAYTKSDRTDKQ
                     VQGLNGTADVLDPASGKKVGEKAVDSGSVAKGKHAEFWGTGLKYDANNLYLAAVYSET
                     QNMTTFGDQGVADKAQNIEAVVQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQDLVK
                     YIDVGATYYFNKNMSTYVDYKINLIDESEFTRKAGVA"
BASE COUNT          313 a          211 c          311 g          338 t
ORIGIN      
        1 ttaatttttg ttcatgacat ctaaatgcat gaatagttca gtttaaataa ggattaaatc
       61 atgaaaaaaa tgacagtggc actttctgct gtagcagtgg cagtgatgtt tgctgcgggg
      121 gcgcaggcag cagaagttta taataaagat ggtaataaac tggatcttta cgggcgtgca
      181 accgctctgc attacttctc ggatgataaa ggtaatgacg gagaccagac ttatgctcgt
      241 ctcggcttta aaggcgaaac gcagattaat gatcaactga ccggatttgg tcagtgggaa
      301 taccagttct ctggtaacaa aacggaatct gaaggttccg cgggaaataa aacccgtctg
      361 gcatttgcag gtctgagatt tgcagatgtt ggtaccattg attacggacg taactatggt
      421 attgcttacg atgtcggatc atatactgac gtactgcctg agtttggtgg tgatggctgg
      481 acgcagaccg ataactttat gacggcccga acttccggtg tcctgactta ccgtaataca
      541 gacttcttcg gactggttga tggtctgaat tttgcggcgc agtatcaggg caaaaatgag
      601 cgtgatgacc ttcagaaggc taatggcgac gggtatggtt tctcggccag ctatgagttt
      661 gatggttttg gttttgtcgc tgcgtatacc aagtcagatc gtactgataa gcaggttcag
      721 gggttgaatg gtactgcaga tgttcttgat cctgcatcag gtaaaaaggt tggagaaaaa
      781 gcagttgact caggtagtgt ggcaaaaggt aaacatgctg aattctgggg aactggtctt
      841 aaatatgatg ccaataacct gtatctggca gcagtgtatt ctgaaaccca gaatatgacg
      901 acctttggtg atcaaggtgt tgcggataaa gctcagaata tcgaagccgt tgttcagtat
      961 cagtttgatt ttggtctgcg cccgtccctt gcctaccttc agtcccgcgg acaggatgtt
     1021 atggttggtg gtgtgaacca tggcgatcag gatctggtta aatatattga tgtcggtgcg
     1081 acttattact ttaacaagaa tatgtccacc tatgttgatt ataaaattaa cctgattgat
     1141 gaaagtgaat ttacccggaa agccggtgtt gcg
//
LOCUS       DAZGAN020000336         1164 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZGAN020000336 DAZGAN020000000
VERSION     DAZGAN020000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.343"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..328)
                     /locus_tag="ACHKYO_RS26590"
                     /old_locus_tag="pgaptmp_005318"
     CDS             complement(<1..328)
                     /locus_tag="ACHKYO_RS26590"
                     /old_locus_tag="pgaptmp_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HGU4431946.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMLS
                     FFGQYKT"
     gene            509..661
                     /locus_tag="ACHKYO_RS26595"
                     /old_locus_tag="pgaptmp_005319"
                     /pseudo
     CDS             509..661
                     /locus_tag="ACHKYO_RS26595"
                     /old_locus_tag="pgaptmp_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
BASE COUNT          372 a          189 c          218 g          385 t
ORIGIN      
        1 gtgttttata ttgtccaaaa aaggaaagca tttttgctag ttctttcatt tctgcaaata
       61 aaatatcagt gcattcacgt tgtgatattt ttgttccgga acttgaggca ctaaaagcaa
      121 cattaataga acctgggcca gggaaatggt atttaataag tctgtaagga tcaacttttg
      181 ctgagatatc gcttaggccg agtgagagaa agtcttgttc taccatatca ccatattgca
      241 tatcatcagc agaaaaatca tcgaataaac taatagtttc gaaaagtgta cagggaatat
      301 taattggcgg aagtaatatg ttattcattt gcatttgcct tggttatatt taagctcaat
      361 atcaattcga gaacaaatgc tagggtaaaa aaatatttaa aaaagaagta aacttcacat
      421 tttttcatta catctctgaa gagaacctcg ttaatgattg gtcttgctca ttgttaataa
      481 caaaatgttg aatttaccga ttaggaaact ttttcctacg cggattgcct ccagctatgg
      541 catgaacttt gagtttatgc cagaactgaa gtggggcttc ggctaccctg gcgcgattat
      601 ctttatgatc ctcgcgggcc tgacaccgta tctgtacttt aagcgtaaga actggctgta
      661 actatacgat gtcgctgtga gtaagttagt gtgctgacag cgacatttta gccgagggaa
      721 tcgcagataa cataattgtt gtgaataata tccatgccac gttgagttgg acgcttaatg
      781 actcacatct caccccttca attgctgcag agataataga aaaaagtgtc aagcccaatg
      841 aaatcaatac aaagtagatt gccttacatc cagtatctct tattcttttt gctccaatat
      901 tgatatttac ataggtatta ttaataacca gaataaaact caaagccagg ttgatatcat
      961 tcaaagttat taatattgac catgatgtta atgtatagat tatgaactga aagcgtgata
     1021 aacgctcaag cctggatggg tagaatatat ttctgatgga tgatttgaac atttttacat
     1081 tgttttatat ataattgttt tgaatatata ttacctgaat tatgtggttt gcatatatat
     1141 atgcaatata gcagtctttg catt
//
LOCUS       DAZGAN020000337         1121 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGAN020000337 DAZGAN020000000
VERSION     DAZGAN020000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.344"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..471
                     /locus_tag="ACHKYO_RS26600"
                     /old_locus_tag="pgaptmp_005320"
     CDS             <1..471
                     /locus_tag="ACHKYO_RS26600"
                     /old_locus_tag="pgaptmp_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4431947.1"
                     /translation="PEAVYARLHEQLGEGAVIHIVPRLAGAGGNGVFQVVLGVAAIVG
                     SFFTGGATLAAWGAALSAGGLTATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNG
                     KQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          312 a          222 c          333 g          254 t
ORIGIN      
        1 ccggaggcgg tgtacgcccg tcttcacgaa caactgggtg agggagcggt catccacatt
       61 gtgccgcgac tggccggagc cgggggaaat ggtgtttttc aggtggtgct gggggttgca
      121 gcgattgtcg ggtcattttt cactggtggt gcaacactgg cggcatgggg cgcagccctg
      181 agtgccggag ggctgactgc caccacgatg ctgttctcac tgggtgccag catgatactg
      241 ggtggtgtgg ctcagatgct ggccccgaag gcaaagacgc cggagtataa aagtacggat
      301 aacggtaaac agaacacgta tttttcgtca ctggacaaca tgattgccca gggtaacccg
      361 atgccggtgc cttacggtga aatgctggtt ggttcacgac ggatatccca ggatatcagc
      421 acccgtgatg agggcggaga cgggaaagtg gtggttatcg ggcggggata aaaataaaaa
      481 aaaatcccgc agagttagcg gagctgcggg aaataacgat gaagattaac gttatggagt
      541 tatttttttg ggcagaaaaa cattaacgca gcgaaattat aagcgccaca tggattttgc
      601 gaaaatgtga agaaattcag aaattttttg ctgcagaggt gcagggagct tgccgggtat
      661 ctgtggcaga agacagaaaa catcatgccg gggttaaggg ataaaaaaat cccgcagagt
      721 ccggagactg cgggacagaa ccgatgaaga ttaacgttca aggagttttg tttttattga
      781 cccgaaaaaa ctgtaacgcc ccggaatgat atctgccacg ggaacgtaca gaaaatgtga
      841 agaaattcag actttttctg tcgcaggttt acagaacgga tgaccgccag ggtaccagtg
      901 gcgtaaatga gcaggatgtt gtggtgtggc tgaagcaaaa aaatctcccg taatatcgcc
      961 ggatactgac gggagacgaa tagtcacctt aaggaggtct gataattatt gcttttattg
     1021 agcaaaaaaa ctgtaacgct gcgtcattat agtggctaca ggcaattgcc ggaaatgtga
     1081 agaatttcag aaattttatt ccgtcatgac acaggcaccc t
//
LOCUS       DAZGAN020000338         1119 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGAN020000338 DAZGAN020000000
VERSION     DAZGAN020000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.345"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..654
                     /locus_tag="ACHKYO_RS26605"
                     /old_locus_tag="pgaptmp_005321"
     CDS             <1..654
                     /locus_tag="ACHKYO_RS26605"
                     /old_locus_tag="pgaptmp_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4431948.1"
                     /translation="AIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDD
                     SHVVTMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQ
                     DKAKKVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGH
                     GQGGMGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            672..1055
                     /gene="quuD"
                     /locus_tag="ACHKYO_RS26610"
                     /old_locus_tag="pgaptmp_005322"
     CDS             672..1055
                     /gene="quuD"
                     /locus_tag="ACHKYO_RS26610"
                     /old_locus_tag="pgaptmp_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HGU4431949.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
BASE COUNT          277 a          224 c          321 g          297 t
ORIGIN      
        1 gcaatccgtc tgtgctggca ctgcgataac cagctgcgcg atcagttcac ggaacggctg
       61 gaatcaatgg caacggataa ctgtgcccgc tgggtgttgt ctgttgtgcg tcgggatctc
      121 ggttttgatg acagtcacgt tgtgacaatg ccggaactgt gctggtggct gattcgtaat
      181 gatctggcgg atgccttacc ggaaagtgca gcccgtaagg cactgagatt accgaagcct
      241 gttgtgccgt ctgttacccg ggaaagtgac cttgtgcctt cggttcctgc caccagcatc
      301 atccaggata aggcgaaaaa ggtgctggcg ctgaaagtgg atccggagtc gccggagtct
      361 tttatgttac gcccaaaacg tcgccgctgg gttaatgaaa agtacacgcg ctgggttaag
      421 acacagccgt gtgcatgttg tggaaagcca gctgatgatc cgcatcatct gataggccat
      481 ggtcagggtg gaatgggtac aaaagcgcat gacctttttg tgctgccttt gtgcagaaaa
      541 caccacgacg aactgcatgc ggataccgtg gcatttgaag agaagtatgg ctcccagctg
      601 gagctgatat ttcgttttat cgatcgcgca ctggcgattg gtgtgctggc ctgattttgt
      661 ggagaaagtt aatgcgtgat attcagatgg ttcttgaacg ttggggagcg tgggcggcta
      721 ataatcatga agatgtgacc tggtcgtcca ttgccgccgg ttttaaggga ttaattcctt
      781 caaaagtaaa atctcgcccg caatgttgtg acgatgacgc gatgattatt tgcgggtgca
      841 tggcccgtct gaaaaagaac aacagcgatt tgcacgattt attagtagat tattatgtag
      901 tcggtatgac attcatgtca ctggcaggta agcattgctg ctctgatggt tatatcggga
      961 aaaggttaca gaaggctgag ggcataattg aagggatgtt aatggcatta gatatccggt
     1021 tagagatgga tatcgttgtt aataactcta attaatatgc caattgttta ctgaaagtta
     1081 ttaaaaatgg ggcgttgcaa cgcccccaaa aataaaggg
//
LOCUS       DAZGAN020000339         1119 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGAN020000339 DAZGAN020000000
VERSION     DAZGAN020000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.346"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..596)
                     /locus_tag="ACHKYO_RS26615"
                     /old_locus_tag="pgaptmp_005323"
     CDS             complement(<1..596)
                     /locus_tag="ACHKYO_RS26615"
                     /old_locus_tag="pgaptmp_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HGU4431950.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGT"
     gene            complement(907..1011)
                     /gene="speFL"
                     /locus_tag="ACHKYO_RS26620"
                     /old_locus_tag="pgaptmp_005324"
     CDS             complement(907..1011)
                     /gene="speFL"
                     /locus_tag="ACHKYO_RS26620"
                     /old_locus_tag="pgaptmp_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HGU4431951.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            complement(990..>1119)
                     /locus_tag="ACHKYO_RS26625"
                     /old_locus_tag="pgaptmp_005325"
     CDS             complement(990..>1119)
                     /locus_tag="ACHKYO_RS26625"
                     /old_locus_tag="pgaptmp_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU4431952.1"
                     /translation="YYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
BASE COUNT          344 a          239 c          238 g          298 t
ORIGIN      
        1 gtaccgttta aaacgaaata ggttttatcg gcattaaaga ctttagccgc atgctgttgt
       61 gcaatacatg gcgcaccttc atgaatcagc agatcgccca ttgctacgtc agcattacac
      121 aagtcagagc ggaagagcat ttcgccaaag tattccacga actgattgcc tgccggatgg
      181 cgacggaaaa actcaccccc ctgatgacca gggcagtcaa aagcactatt gccctgatta
      241 acataatcga ccagtgcgcg gaagaagggc gggcgcagtt gcgtttcata atggctggca
      301 gcggtttcta actgacgtcc ataaaactct ttacgtgatt cgcaatgttc gaagacacca
      361 gaaatacgtg gcagatattc tgcgggtaca cgttcttcat tttccgttgc aataaaaaca
      421 ggaatgtcat agccagtagc gtctatttca tcgagttttc cacgttcaac atcactgact
      481 gataaaataa tggcggcaac gtcaatataa ttactttcgt ctatattcac gcatgcacga
      541 tgagtggaaa aacaatccgg gcaagaacga ctaacggcaa tttttaattc tgacattttg
      601 ttcatctctt tatttcaggt aatagcaggt cctcaatttc tcaaaggaga aatcgatcac
      661 gccggaaaaa aagcaaagct ccaccgctga taaataaatc agtagtttgc tttttgcaga
      721 tgcaataacc cagaccagcc cggtaactgg ctatactcgt catacctcaa gttgcaagtg
      781 ctgcgactcg aattatttag agtttaaaag ggttagcagt atatgagcac cgaggaatac
      841 aggtattaac ctgtaatgct taaagtcagg gagacctgta cgttggggtg ccctttagtc
      901 agaagactag cgggcattaa agaaatgaaa gtcaaaacta ttacgatggg caaacttcat
      961 gatatgggtt gtccgcctta tatgaggcat taagcggtta ttattttcca tttaccttta
     1021 cctcttgctg tgtcagttga aggtagagtt attctatcca tgaaatggct ataatctgtg
     1081 aagaagatca acaaaaaacg atcatacccc aaataataa
//
LOCUS       DAZGAN020000340         1116 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGAN020000340 DAZGAN020000000
VERSION     DAZGAN020000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.347"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..212)
                     /locus_tag="ACHKYO_RS26630"
                     /old_locus_tag="pgaptmp_005326"
                     /pseudo
     CDS             complement(1..212)
                     /locus_tag="ACHKYO_RS26630"
                     /old_locus_tag="pgaptmp_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(243..569)
                     /gene="ybfB"
                     /locus_tag="ACHKYO_RS26635"
                     /old_locus_tag="pgaptmp_005327"
     CDS             complement(243..569)
                     /gene="ybfB"
                     /locus_tag="ACHKYO_RS26635"
                     /old_locus_tag="pgaptmp_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HGU4431953.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMVIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(569..>1116)
                     /locus_tag="ACHKYO_RS26640"
                     /old_locus_tag="pgaptmp_005328"
     CDS             complement(569..>1116)
                     /locus_tag="ACHKYO_RS26640"
                     /old_locus_tag="pgaptmp_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4431954.1"
                     /translation="YITQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMG
                     GTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEA
                     AGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETC
                     KMKCSGNNIGRFIRFVFTGVM"
BASE COUNT          329 a          264 c          200 g          323 t
ORIGIN      
        1 gtccgctcat catccgtgcg gataacccct tccgggatga ggtcggtttt ctccgtcagc
       61 ctgccgtgac ggtcataccg gtaaagatag tgcgcgtcac gggcgatacg gttatccggc
      121 cacatgctga gggcgctgtc cgggtgcagc tccgggtcgg gcaggcggtt acctgccggg
      181 tctgtggcat acgggatatc aatgcccagg ttacatataa taataagagc aattaactta
      241 atttatttgt ttataatgcg cttacttaat acaaaccatt gaatataccc tcctgacaga
      301 aaataaagcc atattattat caccataata tacttactat caatccttac gccaataatg
      361 tctgatacag tccctataaa tattagagca ataaagaata ctatgcctgt tggaaaacat
      421 attaccatca ttccataaga catgagacta attaactcgc tcacatcacg agtggagtct
      481 aatagtgaaa gtctatgcat ggaaaagaat agtattaaaa gcgataaagc cagccatata
      541 gctctgaata aaaaaataat atatttcatt acatcactcc ggtgaataca aatctaataa
      601 aacgtccaat gttatttccg gagcacttca tcttacatgt ttctaaagaa tcattagacc
      661 agtcttggca gtttttaaat agccctaaat ttataggtgt gaatatatca ggcaatacag
      721 aataacgcgg ccttacctct ttaaacttcc cttttaaaca gttctccaca caactattat
      781 cggtttcaac gaggtcacaa cggccagcag cttcttttgt atcattactt gctttacctg
      841 gcccccatag accatctcct tttgattctc cccgttggtc ttggccacca caagtatagt
      901 cccctttacc atctgggaca caaagatatt gatgatagaa cggattcccc catatatctg
      961 gaccgcttct ttcaccagtt ccgcccattg aatgcagtgg cttaatgcat tttagtgttt
     1021 ctaatcctaa tggatctata tttgagatcg gattcaacgg atactgataa aaattccatc
     1081 cccccttcag cccaatcgga tcctgagtga tatacc
//
LOCUS       DAZGAN020000341         1106 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGAN020000341 DAZGAN020000000
VERSION     DAZGAN020000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.348"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..416)
                     /locus_tag="ACHKYO_RS26645"
                     /old_locus_tag="pgaptmp_005329"
     CDS             complement(<1..416)
                     /locus_tag="ACHKYO_RS26645"
                     /old_locus_tag="pgaptmp_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4431955.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETY"
     gene            complement(516..1076)
                     /locus_tag="ACHKYO_RS26650"
                     /old_locus_tag="pgaptmp_005330"
     CDS             complement(516..1076)
                     /locus_tag="ACHKYO_RS26650"
                     /old_locus_tag="pgaptmp_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4431956.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
BASE COUNT          285 a          299 c          242 g          280 t
ORIGIN      
        1 tcatatgtct ccggcagtgg cagaccttca ccgtaagcca taccgtttga ctgaccagca
       61 agaaccacca cgtaatacca ggaaggggag ctggaagcag tttcatttcc tgacggacgg
      121 gtatgtgttt cagcattatc ccagtctgac ggttcagcag taccggcaac atcagcactt
      181 aacggaatga ccgcaccaat acccgctccg gtaatttcca cactcggatc accggatact
      241 ggccttaccc acaggcaatc ccgggtatta aaactgatga gacagtcagc aaatgtcatt
      301 cctccttttg tttcggccgg aggaaaggga gcctcaagaa atgccgctgt accaccggaa
      361 actttaacag taaagtttcc gggcattgat gatagttgaa cccatttgga cgtcataata
      421 atcagcctgt tcctgaataa actgaattat catatccaca gaccgataaa cgccattttg
      481 cgttaattca gtaaaggccc aacaaagaga gcgtcttatt tttccgaaac gctcgcagga
      541 taataacgat aaagggttga aagaccgata tcaaaaataa gtgccacctg tcttcgactg
      601 acacccccgg ccagtaaacg cccgacctgc tcgtattgtt cctgcgtgag acggcggggt
      661 ctgccgccgg ttcgtcccct ttcacgggca gctgcaagac cagcccgtgt ccgttcgaca
      721 attaattcac gctccatttc tgccagcgca cccattatgt gaaaaaagaa gcgccccatg
      781 ggggttgacg tatcaatgtt gtcagtaaga ctacgaaaat ttatcccctg ttgctgtaat
      841 tcatctgtca gcgcaacaag gtggcgcata ctgcgcccca gcctgtccag tttccagacc
      901 atcagcgtgt caccgggttt tagtttcttc agtgcccgct tcagtcccgg ccgctgtgtc
      961 gttttaccac tcattttgtc ttcaaaaatc tgctcacatc ctgcacattc cagcgcattt
     1021 ctttgtaaac cggtgttctg gtcatttgtt gatacgcgta tatagccaat cagcatgtat
     1081 ttaccttcct taaaaagagg tggaaa
//
LOCUS       DAZGAN020000342         1106 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGAN020000342 DAZGAN020000000
VERSION     DAZGAN020000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.349"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..786
                     /gene="waaO"
                     /locus_tag="ACHKYO_RS26655"
                     /old_locus_tag="pgaptmp_005331"
                     /gene_synonym="rfaI"
     CDS             <1..786
                     /gene="waaO"
                     /locus_tag="ACHKYO_RS26655"
                     /old_locus_tag="pgaptmp_005331"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015962475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HGU4431957.1"
                     /translation="SDLAKQYNSRINIYVINCDKLKSLPSTKNWTYATYFRFIIADYF
                     YHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDVEWWQNRASVLTTPQLA
                     SGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFIS
                     EKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPW
                     SKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKCF"
     gene            812..>1106
                     /locus_tag="ACHKYO_RS26660"
                     /old_locus_tag="pgaptmp_005332"
     CDS             812..>1106
                     /locus_tag="ACHKYO_RS26660"
                     /old_locus_tag="pgaptmp_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HGU4431958.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQ"
BASE COUNT          397 a          159 c          192 g          358 t
ORIGIN      
        1 tctgatttag caaaacaata taattcaaga attaatattt atgttatcaa ttgtgataag
       61 ctgaagtcat taccaagcac gaaaaactgg acttacgcaa catattttcg atttataatt
      121 gcagattatt tctatcataa acatgaaaaa atactatatc ttgatgcaga tattgcttgc
      181 aagggtagta ttaaagaact cttagattat caattttcta ctaatgaaat tgccgcagtt
      241 gtagctgaaa gagatgttga atggtggcaa aatcgagcct cggtattaac tacaccacag
      301 ttagcttctg gatatttcaa tgctggtttt ttactgataa atattgatga gtggaatcta
      361 aataacattt cgtcaaaagc tattgaaatg ttgcgtgacc cagattgggt aagtaaaatc
      421 acccaccttg atcaagatgt actgaatgta ttattgaatg gtaaagtgaa gtttatttcg
      481 gagaaatata atacccgata tagtattaac tatgaattaa aagacaaagt tgataatcca
      541 gtcaatgatg acaccgtgtt tatacactat gtgggaccta caaaaccctg gcatgagtgg
      601 gctgactatc cggtgtcacg tagttttttg atcgccaaag cagcttctcc gtggagtaaa
      661 gaagatttac ttaaacctgt aaatagcaat cagtatcggt attgtgcaaa acataaattt
      721 aaacaaaagc attatatggc aggcattttt aattatttaa agtattataa agaaaaatgc
      781 ttctaaagat ttaaggttaa aataattcat tatgatatat aataaaacga taaatgggtt
      841 gaaagttttc ataaaagata atgatccctt ttatgagcaa gttctaaatg actttttaac
      901 ttgcagggtt aaaacattaa aagtttttcg ttcgattgat gatactaaag tcattcttat
      961 tgacactgct cgtggacctc ttgtacttaa agtttatgcg ccaaagcata aaatgaccga
     1021 acgtttctta aaatcttgca taaaaaaaga ttattatgaa aatttaattt atcaaaccga
     1081 tcgggtacgt ggtgaaggca tccaat
//
LOCUS       DAZGAN020000343         1103 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGAN020000343 DAZGAN020000000
VERSION     DAZGAN020000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.350"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..456
                     /locus_tag="ACHKYO_RS26665"
                     /old_locus_tag="pgaptmp_005333"
     CDS             <1..456
                     /locus_tag="ACHKYO_RS26665"
                     /old_locus_tag="pgaptmp_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU4431959.1"
                     /translation="RGDIHRFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFV
                     REDDWWRNGQNLYLDNLAENGFYRVSPSRAQAGDILLCCFGSSVPNHAAIYCGNGELL
                     HHIPEQLSKRERYSEKWQRRTHSVWRHRHWSASAFTGIYNDLVVASACM"
     gene            453..1034
                     /locus_tag="ACHKYO_RS26670"
                     /old_locus_tag="pgaptmp_005334"
     CDS             453..1034
                     /locus_tag="ACHKYO_RS26670"
                     /old_locus_tag="pgaptmp_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4431960.1"
                     /translation="MNTAAEAIRALSLQVPGFRCQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEPLSGRAVIHIVPRLAGAGGNGVFQVVLGAAAIVGSFFTAGATMALWGAALSAGGL
                     TATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          232 a          270 c          358 g          243 t
ORIGIN      
        1 cggggtgaca ttcaccggtt ccgctgtgtg ccgcacctga ccggacggcg ctttgaacac
       61 ggtgtgacgg actgttacac cctgttccgg gatgcttacc atctggcggg gataacgctg
      121 ccggattttg tgcgtgagga tgactggtgg cgcaacggtc agaacctgta cctggacaac
      181 ctggcggaaa acggctttta ccgggtgtct ccgtcccgtg cacaggcagg cgatattctg
      241 ctgtgctgct ttggttcatc ggtgccgaat catgccgcca tttactgtgg caacggtgaa
      301 ctgcttcacc atatacctga acaactgagt aaacgggaga ggtattcaga gaaatggcaa
      361 cgacgaacgc attctgtctg gcgtcaccgc cactggtccg catctgcctt cacggggatt
      421 tacaacgatt tggtcgtcgc atcagcctgt atgtgaacac ggcagcggag gccatccgtg
      481 ccctgtcgct gcaggtgccg ggattccgct gtcagatgaa cgaaggctgg tatcagatac
      541 gtattgccgg tgaggatacc gcgccggagg cggtgtatgc ccgtcttcat gagccactga
      601 gcgggagggc cgtgattcat attgtaccga ggctggcagg ggccggggga aatggtgttt
      661 ttcaggtggt gctgggggct gcagccatcg tgggttcttt cttcaccgcc ggtgcaacga
      721 tggcgttgtg gggcgcagcc ctgagtgccg gagggctgac tgccaccacg atgctgttct
      781 cactgggtgc cagcatgata ctgggtggtg tggctcagat gctggccccg aaggcaaaga
      841 cgccggagta caaaagtacg gataacggta aacagaacac gtatttttcg tcactggaca
      901 acatgattgc ccagggtaac ccgatgccgg tgccttacgg tgaaatgctg gttggttcac
      961 gacggatatc ccaggacatc agcacccgtg atgagggcgg tgacgggaaa gtggtggtta
     1021 tcgggcgggg atgaaaataa aaaaatcccg cagagttagc ggagctgcgg gagagaacga
     1081 tgaagattaa ctttatggag tta
//
LOCUS       DAZGAN020000344         1100 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGAN020000344 DAZGAN020000000
VERSION     DAZGAN020000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.351"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..841
                     /locus_tag="ACHKYO_RS26675"
                     /old_locus_tag="pgaptmp_005335"
     CDS             <1..841
                     /locus_tag="ACHKYO_RS26675"
                     /old_locus_tag="pgaptmp_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGU4431961.1"
                     /translation="AVLNKVITDVGPGKLNLGGCVIEVMGTYNAAIVRVTIGEYGATG
                     FINGTVCTCTVYGDTQYFHWRVDYSTKNKPDTVSQRDASTTQKGVVQLSSDTGSNDET
                     KAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDPGAQGPKGETGATGPAGP
                     RGAQGPKGEKGDPGPRGERGETGPMGPPGASDGKSRVVGIRLGNKQIYAPQTDSTTWA
                     VDLDIGAMITGIGGYNDGNKTLIDRVSYRPLQVTFDGSQWRTVSVGEYVSSGASGFEY
                     FPL"
     gene            883..>1100
                     /locus_tag="ACHKYO_RS26680"
                     /old_locus_tag="pgaptmp_005336"
     CDS             883..>1100
                     /locus_tag="ACHKYO_RS26680"
                     /old_locus_tag="pgaptmp_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4431962.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGISRVASVLWPVGQSVVEVA"
BASE COUNT          292 a          230 c          366 g          212 t
ORIGIN      
        1 tgcggtactc aataaagtca tcacggatgt cggaccggga aaactcaatc tgggtggctg
       61 tgtcattgag gtgatgggga cgtataacgc agccatagtc cgggtcacca ttggcgaata
      121 cggtgcaacg gggtttatta atggcacggt ctgtacctgt acggtttacg gcgacacaca
      181 gtatttccac tggcgggtgg attacagcac aaaaaacaaa ccggatacgg tcagccagcg
      241 cgatgccagc acgacgcaga aaggtgtggt gcagttaagc agtgatactg gcagtaatga
      301 cgaaacaaag gcagccacgc cgaaggccgt gaaggcggca atggatgtgg caaatgaagc
      361 gaaaacaaag gcagaagagg ctgcagcagg aggtggtgtt cccggtccga aaggagagaa
      421 aggcgacccc ggagcacagg gtccgaaagg tgaaacggga gcaacaggcc ccgctggtcc
      481 gcggggagcg cagggaccga aaggggagaa aggcgacccc ggtcccagag gtgagcgtgg
      541 agaaaccggg ccgatggggc cgcccggggc atcagacggg aagagccggg ttgtgggtat
      601 caggctgggt aataaacaga tttatgcgcc gcaaacagac agcactacat gggcagtgga
      661 tttggatatt ggtgcaatga ttacggggat tggcggttac aacgacggaa acaaaaccct
      721 tattgacagg gtatcttaca ggccgcttca ggtgacattt gatggttctc agtggcgtac
      781 agtcagcgtg ggtgagtatg tgtcgtcagg agcttcgggg tttgagtatt ttccattata
      841 aagcgcatca tctttcagac atgtaaccgg gaggacttac agatgcacat aaagaatttc
      901 agacaataca cgccggaaaa tccggatgtg ccgggtgcga tgtacctgaa atcagaggat
      961 ggtcaggact ggtatgagtg tcagtcgtta ttttcagcag agacgctgaa ggtggtttat
     1021 aacagtgccg gagtcattac cggcatcagc agggtggcat cagttctgtg gccagtgggc
     1081 cagagtgtgg tggaggtggc
//
LOCUS       DAZGAN020000345         1079 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGAN020000345 DAZGAN020000000
VERSION     DAZGAN020000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.352"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..587)
                     /locus_tag="ACHKYO_RS26685"
                     /old_locus_tag="pgaptmp_005337"
     CDS             complement(<1..587)
                     /locus_tag="ACHKYO_RS26685"
                     /old_locus_tag="pgaptmp_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4431963.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYQPCVNISAEPE
                     AYFRITPEDWLQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHR
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLADN
                     GFYRVSPSCAQAGDILLCCFGSSVPNHAAIYCGNG"
     gene            complement(598..>1079)
                     /locus_tag="ACHKYO_RS26690"
                     /old_locus_tag="pgaptmp_005338"
     CDS             complement(598..>1079)
                     /locus_tag="ACHKYO_RS26690"
                     /old_locus_tag="pgaptmp_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4431964.1"
                     /translation="SARPSLTVSNLFGLVTGMAEDLQSLVGATVVRRRVYARFLDAVN
                     FVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDGALFPGRIMLANTCMW
                     DYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNVGNFGGFLSINKLSQ"
BASE COUNT          234 a          369 c          283 g          193 t
ORIGIN      
        1 tcaccgttgc cacagtaaat ggcggcatga ttcggcaccg atgaaccaaa gcagcacagc
       61 aggatatcgc ccgcctgtgc acaggacgga gacacccggt aaaagccgtt gtctgccagg
      121 ttgtccaggt acaggttctg accgttgcgc caccagtcat cctcacgcac aaaatccggc
      181 agcgttatcc ccgccagatg gtatgcatcc cggaacaggg tgtaacagtc cgtcacaccg
      241 tgttcaaagc gccgtccggt caggtgcggc acacagcgga accggtgaat ttcaccccgg
      301 cagaccagcc accagggcag ggcactcttt atctgcagcc gccggtccgc ctcgctcagc
      361 cagggcagac caccgggatg actgtggacc agcgccacaa tctccccctg catctgtgcc
      421 tgcagccagt cttccggtgt aatacgaaaa tacgcctccg gctctgcgga gatattcacg
      481 cagggctggt accgttcgcc ctccggggtg cttatcacga agccgcacga ctccgctggc
      541 gcacaccgcc gggcatgcgc cagaatcgct gattcagtct gtgtcataaa acaggattta
      601 ctgcgaaagt ttattgatgg aaaggaaacc gccaaaattg ccgacattcc tgcgcagttc
      661 acacccgcgc atgcacttgc tgcatctgtc cttccggata tccgtggtgg ggttgtcgaa
      721 ctcatccgcc actgccggac cgttataccc gcattcatcg ccccggtaat cccacataca
      781 ggtgttcgcc agcatgatgc gaccgggaaa cagcgccccg tccgtctccg tcggtgtcgc
      841 cagcacaaac gaggccgtca tggccgtcag cgatgacatc tgctccacca cccaccggtc
      901 cgtcagctcc tgctccgggt cggcctcagg attgcctgcc acaaaattca ccgcatccag
      961 aaaacgcgca tacacccggc gacggaccac cgtggcaccc accaggctct gcaaatcctc
     1021 cgccatcccg gtgacaaggc caaacagatt ggacaccgtc agcgacgggc gggcactgc
//
LOCUS       DAZGAN020000346         1060 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGAN020000346 DAZGAN020000000
VERSION     DAZGAN020000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.353"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            509..>1060
                     /locus_tag="ACHKYO_RS26695"
                     /old_locus_tag="pgaptmp_005339"
     CDS             509..>1060
                     /locus_tag="ACHKYO_RS26695"
                     /old_locus_tag="pgaptmp_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4431965.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVLVKPSDGEGTVISTTSEPEYYFVVVLAGQSNSMAYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQ
                     YGTVGQGLHIAKKLLPFIPANAGI"
BASE COUNT          250 a          255 c          299 g          256 t
ORIGIN      
        1 attgctggtt tcgtagtgtg cagcaatgta gttacagtgt aatcaatgtc acaattcagt
       61 cagttgacgg ttgcctgtct ggctgagcat ttgttaaaaa aatcctgcat gatgaatccc
      121 cctgtgcgga ggggcataat gacagatgtt tggttgcgta ttgtataggc aagttgcgga
      181 ttctgtctgg tcattgcaga attcaccggg aggcacccgg catcatgctg tatacagaga
      241 ttaggcatat atccaggctc ctcatcgcag gagccttttt acatgcaaaa aaagcccgcg
      301 ccgggagacg cgggcggcaa ggaataaaca acaaaacgtg aagtaatatt tcagctggcg
      361 aataataccc gacagtaatc actctgcgca actgcgcggc ctttttcgta ttgcgggctg
      421 tagtcttcct tctgtcattg tcctttaact tccggacctc agcccgctct ttatctgatt
      481 cagtacacta tcccggccgg gaggattcat gacatttaaa cactatgacg tggtcagggc
      541 ggcgtcgccg tcagaccttg cggatgcgct tgctcaaaaa attcgtgaag gatggcagcc
      601 atacggcggg ccgttttctt cgtatacgga tgatggcgca gcacttattc aggcgattgt
      661 cgcagaaggt gatgtgagca cacctgtgtt ggtgaagccg tcggatggag aaggcacagt
      721 tatcagcacc accagcgaac cggagtatta ctttgttgtg gttctggcgg ggcagtcaaa
      781 cagcatggca tatggtgaag gccttccgct gccggagaca tatgaccgtc cggacccgcg
      841 tattaagcag ctggcgcgcc gcagtacggt gacaccgggc ggtgccgcct gtaagtataa
      901 cgacattatt ccggcggacc attgtctgca tgatgtgcag gacatgagcc gtcttaacca
      961 tccgaaagcg gacctgtcaa aggggcagta cggaaccgtg gggcaggggc tgcatatcgc
     1021 caaaaaactg ctgccgttta taccggcgaa tgcgggcatt
//
LOCUS       DAZGAN020000347         1057 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGAN020000347 DAZGAN020000000
VERSION     DAZGAN020000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.354"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            148..402
                     /gene="yjbL"
                     /locus_tag="ACHKYO_RS26700"
                     /old_locus_tag="pgaptmp_005340"
     CDS             148..402
                     /gene="yjbL"
                     /locus_tag="ACHKYO_RS26700"
                     /old_locus_tag="pgaptmp_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HGU4431966.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            426..1057
                     /locus_tag="ACHKYO_RS26705"
                     /old_locus_tag="pgaptmp_005341"
                     /pseudo
     CDS             426..1057
                     /locus_tag="ACHKYO_RS26705"
                     /old_locus_tag="pgaptmp_005341"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
BASE COUNT          364 a          157 c          213 g          323 t
ORIGIN      
        1 ataaattgct tcaagttaac tcgctattta tttaactggg tttaaacgca tacctgtagt
       61 tcagaaatta tgagcgattt ttgatagtga ccagatggca aaagaattat aaggaggaca
      121 cggtacgaca aaaaaggtag atgaatcatg ttgaagataa tacccggtgc aactggatac
      181 tttaataaaa cactcaattc aaatcaattc gataatgaag atgcaatcaa agataaatta
      241 gataatagag gttcaatcaa aggtaaatta aataatatat atggtaaatc tattgattat
      301 gcagcgctgc gtcatcggga tataattatt gcaaaaatag atttgtttat tcaacgaatc
      361 acgcataatt tatggcatgc acgtaaaaaa atgtgttttt aattgagcaa ataaatgatc
      421 tcaaaatgtg ggtcaataag tatattgacg attgcactga tgaggattta aacgatcgtg
      481 actttattgc atcagttgtt gaccgggcta tttttcattt cgcgattaat agtatatgta
      541 atcctgggga taataaagat gcgactccca ttgaacgatg tacttttgat gtagaaacta
      601 agaatggcct tccctccacg gttcagctat tttatgagga atctaaggat aatgaacctt
      661 tagcgaatat acattttcaa gcaataggtt ctggtttttt aacgtttgtt aatgcctgcc
      721 aggaacatga tgacaacagc ttaaaattat ttgcttcgct gttaatttca ctttcatatt
      781 ctagtgccta cacagattta gccggagcag aaaaagtgaa tattaatgaa tataatgaga
      841 actatctgac agctcagttt gaagaattat ctcaacgtga tatgaagaag tacctgggag
      901 agatgaagca tctggcggac cgggggggat gaaatttgat gactatctgg ataaaatgtc
      961 acttctggtg aatgaaggaa agctcgaacc tgatatttta agcaaaatgc gagatgctgc
     1021 accgaaatta attgactttg ctaagtcgtt tgaccca
//
LOCUS       DAZGAN020000348         1052 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGAN020000348 DAZGAN020000000
VERSION     DAZGAN020000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.355"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            47..556
                     /locus_tag="ACHKYO_RS26710"
                     /old_locus_tag="pgaptmp_005342"
     CDS             47..556
                     /locus_tag="ACHKYO_RS26710"
                     /old_locus_tag="pgaptmp_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HGU4431967.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSALLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQNPVNRALKPGTVTAKIRAIWKVMLRQGFITDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            626..1051
                     /locus_tag="ACHKYO_RS26715"
                     /old_locus_tag="pgaptmp_005343"
     CDS             626..1051
                     /locus_tag="ACHKYO_RS26715"
                     /old_locus_tag="pgaptmp_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HGU4431968.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
BASE COUNT          259 a          279 c          304 g          210 t
ORIGIN      
        1 atgaggcaga aaaggctttc catgtccgta aaaaggtaaa tacggtatga gaggaaaact
       61 gatatccgcc attcatgtgg caaagcgcga gcttgccctg gatgatgaga cttatacatc
      121 cgcgctgctg gcagccaccg gcaaaaccag ctgccgggat atgtcaccgg atgagttatc
      181 ccgcgtgctg gatgttttca aaaaacgcgg ttttaaagtg cgtcagaacc cggttaaccg
      241 ggccttaaaa ccgggtacgg tgaccgccaa aattcgcgcc atctggaagg tgatgctccg
      301 gcagggcttt atcaccgatg gtgcggaaac cgcccttaac cgctgggtga aatcgcagac
      361 ggccgcgcag aacggcggcg aaggtgtggc aaactggcag tggctggagc aacaccccgc
      421 cctggcctca gatgtgctgg agcgtctcaa gcgatggcac cgccgcaaaa tgctggccgc
      481 gatgggaatg cccgaacgca cgctgatggg gtatgacgcc gtttgcaggc agtatgaaaa
      541 atcacttccc cgttaacccc aaatcccgcc acaacgcggg atttttattt taaacttacc
      601 gggaacgcga gaaccggagg ctgatatggc agaaactcag atgagcatgt ttggtggtga
      661 cagtgagcaa ctgcacgccc ttatcgaccg ccttgatgac atccctgatg atgttctcaa
      721 aaagaactgg ccgcggaccc tgtcagaact ggttgaagtc accggcgcag aacttcagcg
      781 tcaggggatt gagccggtac tggccggtaa actggcacgc aaggtggctg cggctcaggc
      841 agcctatatg ggcggacggg gttattacct gccggtcggg gaatctctct ttgccgagct
      901 gcgaaacaat gagatatttt cgcgctggga ccggggcgag aaaattgaat cccttcgccg
      961 ccattaccgg atgtcagaaa cccagattta taccgtcata cgcgaacagc gccgtctgca
     1021 tctggcaaga acgcaaccgc cacttttctg at
//
LOCUS       DAZGAN020000349         1044 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGAN020000349 DAZGAN020000000
VERSION     DAZGAN020000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.356"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            89..364
                     /gene="yddL"
                     /locus_tag="ACHKYO_RS26720"
                     /old_locus_tag="pgaptmp_005344"
                     /pseudo
     CDS             89..364
                     /gene="yddL"
                     /locus_tag="ACHKYO_RS26720"
                     /old_locus_tag="pgaptmp_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            403..>1044
                     /locus_tag="ACHKYO_RS26725"
                     /old_locus_tag="pgaptmp_005345"
     CDS             403..>1044
                     /locus_tag="ACHKYO_RS26725"
                     /old_locus_tag="pgaptmp_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGU4431969.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFNATNYDRLVKLSLNSNTLESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNK"
BASE COUNT          375 a          194 c          172 g          303 t
ORIGIN      
        1 ttttaaaaac agtatgtcat ttataaatga cgccgtttaa aattcgttta gaaaatacgt
       61 ttaacgataa ttaaaaggat tctgttaaat gaaattaaaa atagttgcgg tggttgtaac
      121 tggtttgtta gctgcgaacg tagcacacgc tgccgaagtc tataacaagg atggtaataa
      181 actcgacctt tatggcaagg ttaccgctct acgttatttt actgatgata agcgtgacga
      241 tggtgataaa acttatgccc gtctcggctt taaaggagaa acgcaaatca atgatcaaat
      301 gattggtttt ggtcactggg aatatgattt taaaggctat aacgatgaag ccaacggctc
      361 gcgcggcaag aatctctgat tgagttaagt gcccatcaca atatgatcac tgaccttata
      421 ttacacaatc atcccaggat gaaaacaatc actttaaacg acaaccatat tgcacattta
      481 aacgccaaaa acactacaaa actggaatat ttaaacttaa gcaataacaa tttactgcca
      541 accaatgaca ttgatcaact aatatcatca aaacatcttt ggcatgtatt agttaacgtc
      601 atcaacaatg atccacttgc acaaatgcag tactggactg cagtaagaaa tataattgat
      661 gacactaatg aagtgaccat tgatttatca ggacttaatt taaccactca accaccaggg
      721 ctgcaaaact tcacctctat caatcttgat aataaccaac tcacacattt taatgcaacc
      781 aactacgata gactcgtaaa gctaagtctg aatagtaata ctcttgagtc aataaatttt
      841 cctcaaggca gaaatgtaag tattacacat atatctatga ataataatgc tctcagaaat
      901 attgatatag ataggctttc atcagttact tattttagtg cggcacataa tcaactagag
      961 tttgtgcaat tagaatcttg cgaatggctg caatacctga atctcagcca taatcaatta
     1021 actgatattg ttgcaggtaa taaa
//
LOCUS       DAZGAN020000350         1026 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZGAN020000350 DAZGAN020000000
VERSION     DAZGAN020000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.357"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            21..923
                     /locus_tag="ACHKYO_RS26730"
                     /old_locus_tag="pgaptmp_005346"
     CDS             21..923
                     /locus_tag="ACHKYO_RS26730"
                     /old_locus_tag="pgaptmp_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGU4431970.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVWFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEAVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            920..>1026
                     /locus_tag="ACHKYO_RS26735"
                     /old_locus_tag="pgaptmp_005347"
     CDS             920..>1026
                     /locus_tag="ACHKYO_RS26735"
                     /old_locus_tag="pgaptmp_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431971.1"
                     /translation="MKYDGDGRVTARFFSGKGCRRAPLFTAPADAARHKR"
BASE COUNT          213 a          247 c          322 g          244 t
ORIGIN      
        1 caaactggtg cagtaaccgt atgcattttt ctggtgaacc ggcacagatt gctgagatta
       61 aacgactggc cagtggtgcc gtcacaccgt tttatcgccg cgccacaaat gaaggtattc
      121 agctgtttct ggccggaagt gccggacttc tgcagaccac tgaagatgtg tggtttgaac
      181 cgtgccccgg actgacggct gccggacgtg gcgttgtatc gccggagaat atcgcgttca
      241 cccgctggct gacacacctg cagaacggtg tactgctgga tgagcaaaac tgcctgatgc
      301 tgcatgaact ctggctgcag agcggtactg gccagcgtcg ctgggaagga ttaccggacg
      361 atgtcaggga taccatcacc gcgcttttca ccgcaaaaag aggtgactgg tgtggcttct
      421 ggagtaacga ggctgtatcg gtgtggtgga accgtctgtg tgacaacgta ctgccggaaa
      481 aaaccatgcc gtttgacctg ctgacggttc tgcccacccg ccttgatgtt gaagtgaatg
      541 gctttaacgg tggtgttctg aacggtgttc cttctgcata tcactggtat accgaacagt
      601 atggcgtgaa gtggccttgt gggtatgacc tgaatatcag cagtcaggga gacaacttca
      661 ttcaggttga tttcgacacg ccgtggtgtc agccggaaag cgacgttatt gcagaattaa
      721 gccgccgttt cagctgcacg ctggagcact ggtatgccga acagggctgt gatttctgtg
      781 gctggcagtt gtatgagcgc ggagagctcg ttgatgtgct gtggggggag ctggaatggt
      841 cttccccgac agatgacgat gagctgccgg aagtcaccgg acctgcgtgg atagtcgaca
      901 aggtggcgca ttatggcgga tgaagtatga cggagacggg cgggttaccg cccgtttctt
      961 ttccggtaaa ggatgtcgcc gtgctcctct ttttactgcg ccggctgacg cggcgcggca
     1021 taaacg
//
LOCUS       DAZGAN020000351         1024 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGAN020000351 DAZGAN020000000
VERSION     DAZGAN020000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.358"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            173..>1024
                     /locus_tag="ACHKYO_RS26740"
                     /old_locus_tag="pgaptmp_005348"
     CDS             173..>1024
                     /locus_tag="ACHKYO_RS26740"
                     /old_locus_tag="pgaptmp_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HGU4431972.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRL"
BASE COUNT          246 a          256 c          295 g          227 t
ORIGIN      
        1 agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg ccttgggttc
       61 taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat aaaccggaca
      121 gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca ctatggaaca
      181 tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg atgtgttgcg
      241 tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc acgatgagct
      301 ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg aagcgaccgg
      361 cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag tgtcagtcat
      421 taatcctgcg ctgggtaaag ctttcgctca gagtgaagga ctgcgtaaca agactgatac
      481 cgtggatgcg cgcatgctgg cagagttctg tcgtcagaag cgccctgcag cctgggaagc
      541 gcctcacccg cttgaacgcg cgttgcgtgc cctggtagtc cgccaccagg cgctgacaga
      601 tatgcacacg caggaactga atcgcactga aacggcgcgg gaagtccaga gaccgagcat
      661 tgatgctcac cttctgtggc ttgaagcaga gctgaagcgt cttgagaagc agataaaaga
      721 cctgacagac gatgatccgg atatgaaaca ccgcaggaaa ctgctggaaa gcatcccggg
      781 tatcggagag aaaacatctg cggtattgct ggcttatatc ggtctgaagg accgcttcgc
      841 ccatgccaga cagttcgccg cttttgcggg tctgacacca cggcgttatg aatcaggtag
      901 cagtgtgaga ggggcgagcc ggatgagtaa ggccggacat gtgtcgcttc gcagggcgtt
      961 gtatatgccc gcaatggtag ccaccagtaa gactgagtgg ggacgggcgt tccgcgaccg
     1021 tctg
//
LOCUS       DAZGAN020000352         1022 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGAN020000352 DAZGAN020000000
VERSION     DAZGAN020000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.359"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..381
                     /locus_tag="ACHKYO_RS26745"
                     /old_locus_tag="pgaptmp_005349"
     CDS             <1..381
                     /locus_tag="ACHKYO_RS26745"
                     /old_locus_tag="pgaptmp_005349"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU4431973.1"
                     /translation="EYVIVKNVPYDEYVKSFSRAHLFIDQCYSYDKGVNALLGMAAGK
                     VVFSGFEPEAKRYYEFFDDKYTPLINAVPDEDTIFLQIEDLLLNKQKINNISAAAREF
                     ILRYHLSEDVADNFLNVWGYDIHD"
     gene            374..>1022
                     /locus_tag="ACHKYO_RS26750"
                     /old_locus_tag="pgaptmp_005350"
     CDS             374..>1022
                     /locus_tag="ACHKYO_RS26750"
                     /old_locus_tag="pgaptmp_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGU4431974.1"
                     /translation="MTKFSLVLCTINNRKDIPKFIESLSCNIHEKKFEVELIVIDQNT
                     LSIADLFSDIAERVTLKYFKVDFKGLSRARNYALKHVSGDIVCFPDDDCIYTQSLLKN
                     IYELFAYNNHVDFISVNTKDPIAGEKALVKLPKSKTNISFKSKNCVSFTLFFRREVIN
                     AVGRFDQNMGVGAGTNYGAGEESDFVVRALSLGFKGLYYPDYYVLHPAKESHPVFD"
BASE COUNT          339 a          143 c          195 g          345 t
ORIGIN      
        1 gaatatgtta ttgttaaaaa tgtaccttat gatgagtatg ttaagtcttt ttcacgtgcg
       61 catcttttca ttgatcagtg ctacagttac gataaaggag tcaacgcatt actcggaatg
      121 gctgcgggaa aggtggtttt tagcggcttc gaaccggagg caaaacgata ttatgaattt
      181 tttgatgata aatacacacc attaataaat gctgttccag acgaggatac tatttttctt
      241 caaatagaag atttgttatt aaataaacaa aaaataaata atatttcagc agcagcaaga
      301 gagtttatcc tacgttacca tttgtctgaa gatgttgctg ataattttct gaatgtttgg
      361 ggatatgata tccatgacta aattttccct tgtcttatgc actataaata ataggaaaga
      421 tatcccaaaa tttatcgaat cattatcatg taatattcat gaaaaaaaat ttgaagtaga
      481 gttaattgtt atagatcaaa atacattgtc gattgcggat ttattttctg atatagctga
      541 aagagtgact ttaaaatact ttaaagtaga tttcaaaggg ttgtcacgtg caagaaatta
      601 tgctttaaaa catgtttcag gcgatattgt atgttttcca gatgatgact gcatttatac
      661 acagagtcta ctaaaaaata tttatgagtt atttgcatat aataatcatg ttgattttat
      721 cagcgttaat actaaggatc ccattgcagg ggaaaaagca ctggtcaagc tacctaaaag
      781 caagactaat atttcattca agagtaaaaa ttgtgtttca tttacattat ttttcaggcg
      841 cgaagttata aatgctgttg gtagatttga tcaaaatatg ggggtcggag ctggcactaa
      901 ctatggtgca ggagaagaaa gcgactttgt agttcgggcc ctttctttag gttttaaagg
      961 tctgtactat cctgactatt atgtattaca tccagcgaaa gaaagccatc cagtatttga
     1021 tg
//
LOCUS       DAZGAN020000353         1020 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGAN020000353 DAZGAN020000000
VERSION     DAZGAN020000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.360"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..222)
                     /locus_tag="ACHKYO_RS26755"
                     /old_locus_tag="pgaptmp_005351"
     CDS             complement(<1..222)
                     /locus_tag="ACHKYO_RS26755"
                     /old_locus_tag="pgaptmp_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGU4431975.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASE"
     gene            611..844
                     /gene="ynfO"
                     /locus_tag="ACHKYO_RS26760"
                     /old_locus_tag="pgaptmp_005352"
     CDS             611..844
                     /gene="ynfO"
                     /locus_tag="ACHKYO_RS26760"
                     /old_locus_tag="pgaptmp_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4431976.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLCSEKRVSSEANKEKSDITELLRKQVRPD"
     gene            902..>1020
                     /locus_tag="ACHKYO_RS26765"
                     /old_locus_tag="pgaptmp_005353"
     CDS             902..>1020
                     /locus_tag="ACHKYO_RS26765"
                     /old_locus_tag="pgaptmp_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HGU4431977.1"
                     /translation="MDQVVIFKQIFDKVRNDLNYQWFYSELKRHNVSHYIYYLA"
BASE COUNT          326 a          256 c          189 g          249 t
ORIGIN      
        1 ctcgctggcc tgccgcagtt cttcaacttc ccggcgcagc ttttcgttct caatttcagc
       61 atccctttcg gcataccatc ttataacggc ggcagagtca taaagcacct cattaccctt
      121 gccaccgcct cgcagaacgg gcattccctg ttcctgccag ttctgaatgg tacggatact
      181 cgcaccgaaa atgtcagcca gctgcttttt gttgacttcc attgttcatt ccacggccaa
      241 aaacagagaa aggaaacgac agaggcccaa aagctcgttt tcagcacctg tcgtttcctt
      301 tcttttcagg gggtatttta aataaaaaca ttaagttaca acgaagaaga acggaaacgc
      361 cttaaaccgg aaaattttca taaatagcga aaacccgcga ggtcgccgcc ccgtaacctg
      421 tcggatcgcc ggaaaggacc cgcaaaatga taataattat catctgcatg tcacaacgtg
      481 catctacgcc atcaaaccac gtcaaacaat caattatgac gcagatatcg tattaattga
      541 tccgcatcaa cttaacgtaa aaacaacttc agacaataca aatcagcgac actgaatacg
      601 gggcaacctc atgtcaacga agaacagaac ccgcagaaca acaacccgca acatccgctt
      661 tcctaaccaa atgattgaac aaattaacat cgctcttgag caaaaagggt ccgggaattt
      721 ctcagcctgg gtcattgaag cctgccgccg gagactgtgc tcagaaaaaa gagtttcttc
      781 tgaagcaaac aaagaaaaga gtgacattac tgaattgctc agaaaacagg tcagaccaga
      841 ttgaagcaat ttagataatc gtgcagacta cgccccctca tatcacatgg aaggtttatc
      901 tatggatcag gtagtcattt ttaaacaaat atttgataaa gttcgaaacg atttaaacta
      961 tcaatggttt tattctgagc taaaacgtca caatgtctca cattacattt actatttagc
//
LOCUS       DAZGAN020000354         1013 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZGAN020000354 DAZGAN020000000
VERSION     DAZGAN020000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.361"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..738
                     /locus_tag="ACHKYO_RS26770"
                     /old_locus_tag="pgaptmp_005354"
     CDS             <1..738
                     /locus_tag="ACHKYO_RS26770"
                     /old_locus_tag="pgaptmp_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3672 domain-containing protein"
                     /protein_id="HGU4431978.1"
                     /translation="VKAGVSITSPVIRSAVIQNGNFQVESDGSIKSGEHFSVSVNGDV
                     TASNAKISGHIDADSGTLNNVMIEENCIIKGTMSADRIIGDIVKGYSFEPHDGTLHIT
                     NHKYGEGYVIFKAEVSLADDVSFDRKLILLPFTFYSGTLTIEINGRVVIKADPRVANT
                     GTAGYFDRYDNPVYTRTNLGNTVSGVWDVAAGGGDTRITVTMRVEGGPGNMGDVEGRK
                     RELVRWRYDAFTLMVAKSTDSGFTRIS"
     gene            940..>1013
                     /locus_tag="ACHKYO_RS26775"
                     /old_locus_tag="pgaptmp_005355"
     CDS             940..>1013
                     /locus_tag="ACHKYO_RS26775"
                     /old_locus_tag="pgaptmp_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hok/gef family protein"
                     /protein_id="HGU4431979.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGS"
BASE COUNT          279 a          204 c          284 g          246 t
ORIGIN      
        1 gtaaaggccg gggtaagtat cacttcacca gttatccgga gtgccgttat tcagaacgga
       61 aattttcagg ttgagtccga tggcagcata aaaagcgggg aacatttcag cgtttcagtg
      121 aatggagatg tgacggccag taatgcgaag ataagtggtc atattgatgc ggattccggc
      181 acgctgaata acgtgatgat tgaagaaaac tgcatcatta agggaacaat gagtgcggac
      241 agaatcatcg gagatatcgt caagggatat tccttcgaac ctcatgatgg cacgctccat
      301 attacgaacc acaagtatgg agagggttat gtcatcttta aggcggaggt atctcttgct
      361 gatgatgttt cgtttgatcg caagttgata ctccttccct tcactttcta ttcaggaaca
      421 ctgacgatag agattaacgg aagagttgtc attaaggcag acccaagagt tgctaatacg
      481 ggaacggctg gttattttga cagatacgac aatcctgttt acacgcgcac caacctcggg
      541 aacaccgttt caggagtatg ggatgtggcg gcaggagggg gagatacacg gattacagtg
      601 acaatgagag ttgaaggtgg tcctggcaat atgggggatg tggaagggag aaagagagaa
      661 ctggtgcgat ggagatatga cgcctttact ctcatggtgg cgaaatcgac agattccggt
      721 tttacccgga tatcgtgaca ggcagttgta atacggattg ccggaaggag acaaaaaccg
      781 tacagtatgc gtgggtgcct ttcggctgat ggctggaggg tgaacctgaa ggcctgatgt
      841 ggaaaggccc cgagtcaact ttaacgttaa cccgaggccc tgaccgtaca accttcacaa
      901 gtagtaggtt agcgcctctc cattcaggag acaagcgtta tgtcgcaaaa atcgctcatc
      961 accgtcacaa tttgcatgac ggttatcttc accatctgga tgttgcacgg ttc
//
LOCUS       DAZGAN020000355          993 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGAN020000355 DAZGAN020000000
VERSION     DAZGAN020000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.362"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(151..429)
                     /locus_tag="ACHKYO_RS26780"
                     /old_locus_tag="pgaptmp_005356"
     CDS             complement(151..429)
                     /locus_tag="ACHKYO_RS26780"
                     /old_locus_tag="pgaptmp_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431980.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
     gene            551..973
                     /locus_tag="ACHKYO_RS26785"
                     /old_locus_tag="pgaptmp_005357"
     CDS             551..973
                     /locus_tag="ACHKYO_RS26785"
                     /old_locus_tag="pgaptmp_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HGU4431981.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
BASE COUNT          293 a          241 c          207 g          252 t
ORIGIN      
        1 gatcagcgtc atgtggtcag tgaaatcagt aatggtgtga atcccaccca ggcttactcg
       61 gcggtatacc aggcaagacg cattcgcctc gcccgtaaat atctgaacgg taaaaaggtt
      121 atggaagaaa ccggggaaaa tacgccccca tcagcgtaag gatttctttt gccgctccag
      181 agactccagt tttttacgca aatcctctct tttttgggca tctctggtgc cagccagctc
      241 tgctctcaac tcatcgatct gaagttgtat cttcagtctg ttactgaaca ttttctgtct
      301 ggcattaaca tccgcaacaa tgccgttttt tgtcttctcg gccttttgtt gaaaaacatt
      361 gctgtccgca tgactggcaa ccgaagcaga aagaacacta aaaagcagga ctggcacaca
      421 ttttttcacg ggattattcc tgactcattg accatcaaat cacattggga gtaaaccgac
      481 gtatgataag agatactctt cggatatata actccctgag tatcaagatt aaaaacgcaa
      541 ggagatgttt atgagatctg ccgctgccag gctgcttctg atacctctga taacagcaac
      601 aatagctctt acaggatgca caccaaagac cagcctggaa cgacataccc ggcattatgt
      661 ttatgcttca gatgatggat ttgatcctaa cttctacacc cagaaagcag acaccatacg
      721 tatgatgctc ccgttctttc agcagttccg ggatatgggg atgaaagaca aagctgccgg
      781 agtatcagca gaaacggcac agcaacgtgt aaaagaattc cactcagaaa aattttttca
      841 ctcactccgg agcacaacaa cctttgctgg cagaaaatac acaaacagcg atatgccttc
      901 gccgaaaaaa atgaaactaa tggcagacac catttctgcg gtttatctcg atggatacga
      961 gggcagacag taagggattt accataatcc ctt
//
LOCUS       DAZGAN020000356          983 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZGAN020000356 DAZGAN020000000
VERSION     DAZGAN020000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.363"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..554)
                     /locus_tag="ACHKYO_RS26790"
                     /old_locus_tag="pgaptmp_005358"
     CDS             complement(<1..554)
                     /locus_tag="ACHKYO_RS26790"
                     /old_locus_tag="pgaptmp_005358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431982.1"
                     /translation="MSKKFTKTLLSSSLVGLLFVSAEGFAQVSVYPKDSQYSVLVDDE
                     NKPNKFGSKFKVYYSKSSHRGVVRYVQNGQDNVLFHFGNDGNIFVLSKDGQIGYTVHE
                     PVLKDFARMAEGLKSNGSKNHKLDEAEVQRIYDKIYNMSNTVVNPALKNQVGELSGRV
                     DKIDLAAGAIATDVASKTTVGVNS"
BASE COUNT          290 a          242 c          142 g          309 t
ORIGIN      
        1 tcactgttca caccaaccgt agttttagat gcaacatcag ttgcaatagc tcctgcagcc
       61 agatcaatct tgtcaacgcg accagaaagc tcaccaactt gatttttcaa tgcaggattt
      121 acaacagtgt tagacatgtt ataaatttta tcgtaaatac gctgtacttc agcctcatcc
      181 agcttatggt ttttacttcc attactttta agaccttcag ccatacgggc aaagtccttc
      241 aacacaggct catgaacggt atatcctatt tgcccgtcct tgctcagcac aaaaatattg
      301 ccgtcattac cgaagtgaaa tagaacatta tcttgtccat tttgcacata tcgcacaact
      361 ccccggtgag atgacttact gtaataaact ttgaatttgc taccgaattt attcggttta
      421 ttctcgtcat ccactaaaac actatactgc gagtctttcg gatatactga tacctgtgcg
      481 aaaccctcag cactcacaaa cagaagccct accagagaag atgacagaag tgtctttgtg
      541 aattttttac tcattatgga tatcctgtta aattatctgt atacaaatca gccgcaacac
      601 ctcatactta taaagaaata tgattaatta cgactttatt caggatattc cgaaaataaa
      661 tttaaaaatt aatttaataa ctttaggatt tatcttacgt tattctgggc atacttactc
      721 aggcccacgc ctacaccgcc ggaacagaca tcagaaaaaa cttatcctca atcagacttg
      781 ctgtttcaca tctgcttaca ctgtaatttc tgatcaacat cacaacatta tccgaattta
      841 actgttcagt tcatctacaa tatttccctg cgtgacttta tatcagcccc gcacttccgt
      901 acggggcatg ttctttactc cactcccact tttttattgt actcgcggtt aaaccgatcc
      961 acggcaattt tcatatcccg ctc
//
LOCUS       DAZGAN020000357          979 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZGAN020000357 DAZGAN020000000
VERSION     DAZGAN020000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.364"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..372
                     /locus_tag="ACHKYO_RS26795"
                     /old_locus_tag="pgaptmp_005359"
     CDS             <1..372
                     /locus_tag="ACHKYO_RS26795"
                     /old_locus_tag="pgaptmp_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGU4431983.1"
                     /translation="KTEEWMISQMSFSGAILWINIFSSVLLVPVYEEIVFRGCLFNSF
                     KFWFNDNIYTSAIVTSVIFSALHQQYTDFRTFLMLFLVSLVLISAKIKSNGLLMPILL
                     HMSMNAVIAGIQYLASISYTH"
     gene            complement(547..825)
                     /gene="mcmA"
                     /locus_tag="ACHKYO_RS26800"
                     /old_locus_tag="pgaptmp_005360"
     CDS             complement(547..825)
                     /gene="mcmA"
                     /locus_tag="ACHKYO_RS26800"
                     /old_locus_tag="pgaptmp_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin McmA"
                     /protein_id="HGU4431984.1"
                     /translation="MRKLSENEIKQISGGDGNDGQAELIAIGSLAGTFISPGFGSIAG
                     AYIGDKVHSWATTATVSPSMSPSGIGLSSQFGSGRGTSSASSSAGSGS"
BASE COUNT          319 a          190 c          158 g          312 t
ORIGIN      
        1 aaaacggaag aatggatgat ctcgcaaatg tctttttccg gggccatttt atggattaat
       61 atattttctt ccgtacttct tgttcctgta tatgaagaga ttgtatttcg agggtgtctt
      121 tttaactctt ttaaattctg gtttaatgac aatatttaca cctctgctat tgtaacatca
      181 gtcatatttt ctgcattaca tcaacaatat actgactttc gtacattttt gatgctcttt
      241 ctggtatcgt tagttcttat tagtgcaaaa ataaagtcga atggcctgtt aatgcccata
      301 ttacttcaca tgtcgatgaa tgcagtcata gcaggaatac aatatctggc ttctatcagt
      361 tatactcatt aacagagtat tttctgttac aatctcatat gataaaaatt aaacggactg
      421 gcattgttac tatgcggtat gcataaaata ataaacgttc ggaggagcct aacttaaagc
      481 gttacatagg caccattatc atataatgaa gcacctgtaa catgtgcttc attaacaata
      541 taaggtttaa cttccactcc ccgcagacga agaggcactt gatgtacctc tgccggatcc
      601 aaactgggat gataatccta tacctgaggg agacatggag ggactaaccg tcgcagtcgt
      661 tgcccatgaa tgtactttat cacctatata agcccctgca atagaaccaa atcccgggct
      721 aataaacgta ccagcaagtg aaccaatagc aattaattct gcctgcccgt cattcccgtc
      781 acctccagat atttgtttta tttcattttc agatagtttt ctcatttcaa caccttcgct
      841 ataagatttg tgaagaagta aaaattcacg tttaaccaca acgataaaaa tgtaactaca
      901 agtgataaaa aaaacatata tagattaaaa aaattatatc catagtaata actaagatat
      961 aatgacaccg gaatataaa
//
LOCUS       DAZGAN020000358          979 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZGAN020000358 DAZGAN020000000
VERSION     DAZGAN020000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.365"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>979
                     /locus_tag="ACHKYO_RS26805"
                     /old_locus_tag="pgaptmp_005361"
     CDS             <1..>979
                     /locus_tag="ACHKYO_RS26805"
                     /old_locus_tag="pgaptmp_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4431985.1"
                     /translation="LSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQKADK
                     FAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAE
                     DLLRGNWMAGLRSGWSEWKESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSF
                     TRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGF
                     TGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRA
                     EGINFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQAQMRDGG"
BASE COUNT          235 a          230 c          337 g          177 t
ORIGIN      
        1 agctgtctgc acaggagaaa tccctgctgg cgcataaaga tgagacgctg gagtacaaac
       61 gccagctggc tgcacttggc gacaaggtta cgtatcagga gcgcctgaac gcgctggcgc
      121 agaaggcgga taaattcgca cagcagcaac gggcaaaacg ggccgctatt gatgcgaaaa
      181 gccgggggct gactgaccgg caggcagaac gggaagccac ggaacagcgc ctgaaggaac
      241 agtatggcga taatcctctg gcgctgaata acgtcatgtc agagcagaaa aagacctggg
      301 cggctgaaga cctgcttcgc gggaactgga tggcaggcct caggtccggc tggagcgagt
      361 ggaaagagag tgccacggac agtatgtcgc aggttaaaag tgcagccacg cagacctttg
      421 atggtattgc gcagaatatg gcggcgatgc tgaccggcag tgaacagaac tggcgcagct
      481 tcacccgctc cgtgctgtcc atgatgacag aaattctgct taagcaggca atggtgggga
      541 ttgtcgggag tatcggcagc gccattggcg gggctgttgg tggcggcgca tccgcgtcag
      601 gcggtacagc cattcaggct gctgcggcga aattccattt tgcgaccggg ggatttacgg
      661 gaaccggcgg caaatatgag ccagcgggga ttgttcaccg tggtgaattt gtcttcacga
      721 aggaggcaac cagccggatt ggtgtcggca acctgtaccg cctgatgcgg ggctatgcgg
      781 aaggtggtta tgtgggcggt gccggaagtc cggcgcagat gcggcgggct gaaggcatta
      841 attttaatca gaacaatcac gtggtgattc agaacgacgg tacgaatggt ctgccaggtc
      901 cacagatgat gaaggcggtg tatgacatgg cccgcaaggg tgcccgtgat gaaatccagg
      961 cacagatgcg cgatggtgg
//
LOCUS       DAZGAN020000359          979 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGAN020000359 DAZGAN020000000
VERSION     DAZGAN020000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.366"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..846
                     /locus_tag="ACHKYO_RS26810"
                     /old_locus_tag="pgaptmp_005362"
     CDS             <1..846
                     /locus_tag="ACHKYO_RS26810"
                     /old_locus_tag="pgaptmp_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4431986.1"
                     /translation="VLADLQCTFEEKIAAQAQTFEEKLASLSVVLQKCVTGDDVRPML
                     EQIVKEAVSHIPVPRDGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMV
                     KEAVSHIPVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPR
                     GTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKT
                     FSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTGDKPGEAHSSGWALAAKRGRD
                     AGGGK"
     gene            843..>979
                     /locus_tag="ACHKYO_RS26815"
                     /old_locus_tag="pgaptmp_005363"
     CDS             843..>979
                     /locus_tag="ACHKYO_RS26815"
                     /old_locus_tag="pgaptmp_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4431987.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMEKVRQATAVLLAYIQGSR
                     D"
BASE COUNT          222 a          233 c          325 g          199 t
ORIGIN      
        1 gtgctggcgg atttacagtg cacatttgag gagaaaatag ccgcgcaggc acaaacgttt
       61 gaagaaaaac tggcttccct gtctgtggta ttacagaagt gcgtgacggg tgatgatgtg
      121 cgtccgatgc ttgaacagat agtgaaggag gcggtaagcc atattcctgt tccgcgtgac
      181 ggtcgtgact acgatccgga agtactgcag aaggcggtga atgatgcggt cgcaaatatt
      241 ccgcagccgg cagacggtaa aagtctcacc ccggatgatg tgcgtccgat gcttgaacag
      301 atggtgaagg aggcggtaag ccatattcct gttccgcgtg atggtcgtga ctatgatccc
      361 gatgttctgc agaaggctgt tctggatgcg gtgagtgccc tgccggctcc gcaggacggg
      421 cgtgatgcca cggcactgga aatactcccc gccattgacg atcaaaaatc ctttccccgg
      481 ggcacgtatg ccacacacca gggcggactc tggcgggcgt atgaaaaaac gcacgggatg
      541 cggggatggg aatgcctggt tgacggggtg gcggatattg acgtcagcat gacgggtgaa
      601 cggttgttct ctgtggtggt ccggcagagc agtggccagc gtacggaaaa aacattttcc
      661 ctgccggtga tgctctaccg cggtgtgttc agagccggtg aaacctacca ccccggcgat
      721 acggtgacgt gggggggctc gctgtggcac tgcaacagta tgaccggtga taaacccgga
      781 gaagctcatt catcaggctg ggccctggct gcaaaacgtg ggcgggatgc aggaggcgga
      841 aaatgacggc attactgacg ctggaagaga tcaaggcaca tctgcgtgtc gaccatgacg
      901 cggatgatga catgctgatg gagaaggttc gtcaggctac cgccgtgctg ctggcctaca
      961 ttcagggcag ccgggataa
//
LOCUS       DAZGAN020000360          973 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGAN020000360 DAZGAN020000000
VERSION     DAZGAN020000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.367"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>973
                     /locus_tag="ACHKYO_RS26820"
                     /old_locus_tag="pgaptmp_005364"
     CDS             <1..>973
                     /locus_tag="ACHKYO_RS26820"
                     /old_locus_tag="pgaptmp_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4431988.1"
                     /translation="ASAFTTGADGTYSESAGASENSLRWGVGKPLYQDLVSRTKAALA
                     KNPKNRLLAVVWMQGEGDAAVGTHAQHPGLFSAMVNQFRTELAGQASQSTGGSASAVP
                     WICGDTTYFWKQRHAEGYASVYGGYKGKESQNIFFVPLMTDENGENVQTNDPAEDPDL
                     EAVGYYGSKWRNDQKTWTSVDRASHFSSWARRGIISDRLATAILVHTGRTAEFITGKQ
                     PDTVKPTGPSGEGPEREPEAPVSNRTLMSLLASGEDLASQGWRYYHKPASGDSVNKNI
                     AEAVVSDEGATGGKALQLNKPESHIWFLEHDAAGQGAELLKKGGRVSV"
BASE COUNT          239 a          225 c          325 g          184 t
ORIGIN      
        1 gtgcttcggc attcacaacg ggtgcagacg gcacatacag cgaatcagcc ggtgcatcgg
       61 aaaattcact gcgctggggt gtgggtaagc cgctgtatca ggatttggtg agccggacta
      121 aagccgcact ggcgaagaac ccgaaaaacc gtctgcttgc ggtggtgtgg atgcagggag
      181 aaggtgatgc ggcagtgggg acgcatgcgc agcatccggg gctgtttagt gcgatggtga
      241 atcagttcag aacggagctt gccggtcagg catcacagag cacaggaggc agcgcatcag
      301 cggtaccgtg gatttgcggg gacacaacgt atttctggaa gcagcgtcat gcggaagggt
      361 atgcatcagt atacggaggg tataagggta aggagtcgca gaacattttc tttgtaccgt
      421 taatgacgga tgagaatggt gagaatgtgc agaccaatga cccggcagaa gacccggacc
      481 tggaagccgt cggttattac ggttcaaagt ggcgtaatga ccagaagacc tggacatctg
      541 tggacagggc cagccatttc agttcatggg ctcgccgtgg gattatttcc gaccgtctgg
      601 caacggcgat tctggtgcat accgggagaa ccgctgaatt cattaccgga aaacagcctg
      661 atacggtgaa gcccaccgga ccttccggtg aaggcccgga gagagagccg gaagccccgg
      721 tcagtaaccg aaccctgatg agtctgctgg cgtccggtga agacctggca tcacagggct
      781 ggcgctatta tcacaaaccg gcgagcggag acagtgttaa taaaaacatc gctgaggctg
      841 tggtcagtga tgagggtgcc accggtggta aggccctgca actgaacaaa ccggaaagcc
      901 acatctggtt tctggagcat gatgccgccg ggcagggggc agagttgctg aagaaaggcg
      961 gacgtgtgag cgt
//
LOCUS       DAZGAN020000361          971 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGAN020000361 DAZGAN020000000
VERSION     DAZGAN020000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.368"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            116..700
                     /locus_tag="ACHKYO_RS26825"
                     /old_locus_tag="pgaptmp_005365"
     CDS             116..700
                     /locus_tag="ACHKYO_RS26825"
                     /old_locus_tag="pgaptmp_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431989.1"
                     /translation="MNMKNSSFLDEGKEPVIERIFRLAERYKSRSEAARAWGINVQTL
                     QNYYKRRNNQPPPQPRKHHLQNIAEREHVSMEWLVHGYGDEPSMKKEPIEQAKRTKKQ
                     RIDDSDSIIMRAWDSLTSSEQEILSNLLIRKGAELLTVLLDTDIQKLHSLSGVKRALA
                     LSLQELPDDTVREIYEECEAKVNHLNVTQKKASA"
BASE COUNT          346 a          172 c          195 g          258 t
ORIGIN      
        1 acgggtaaat aatagaacaa acaaaagaac cagatcaagg tgttttgttc tttttattct
       61 ttgtagtgtt tttgttatta atatcaataa gataaataca aaggaacctg agagcatgaa
      121 catgaaaaac agttcttttc tcgacgaagg aaaagaacca gtgatcgaac ggatttttcg
      181 tcttgcagag cgttacaaat cgagaagtga ggcggctaga gcatggggaa taaacgttca
      241 gacattgcag aactactaca aaaggagaaa caatcagccg ccaccacaac caagaaagca
      301 ccatctccag aacattgcag aaagagaaca tgtatctatg gagtggctag tccatggtta
      361 tggtgatgag ccaagcatga aaaaagaacc aattgaacaa gcaaaaagaa caaaaaaaca
      421 aagaattgat gatagtgatt ccatcatcat gagggcgtgg gactcgctaa cttcatctga
      481 acaagagatt ttgtccaact tattgatcag aaaaggggcc gaactactaa ccgttttgct
      541 tgatacagat attcaaaaac tgcactcatt gagcggagtc aaaagagcac tagcattgag
      601 tctacaagag ttacccgatg atactgtaag agagatttat gaagaatgtg aagctaaggt
      661 taatcattta aatgtaaccc agaaaaaggc tagtgcgtaa gggagtggtg caatttttgt
      721 gctgtagtac actcctttaa agacaatctg gagaaaaacg ttttaagcct ttttaaagcc
      781 attttaagcg tcaatccgta gtatcaaaag ttttgccgat tttagaataa atgattaatt
      841 attgcacaat cggtatcaaa tagactttaa gcgctcatcc agacatcacc tgttttattt
      901 cagtatctta caggtgattt cacctccttt cactaaaacc ctcgttggta tcaaatgatt
      961 cacctggtta c
//
LOCUS       DAZGAN020000362          962 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGAN020000362 DAZGAN020000000
VERSION     DAZGAN020000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.369"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            115..>962
                     /locus_tag="ACHKYO_RS26830"
                     /old_locus_tag="pgaptmp_005366"
     CDS             115..>962
                     /locus_tag="ACHKYO_RS26830"
                     /old_locus_tag="pgaptmp_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431990.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYTLQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMLARDQPVMAALGKAIDAGDHDAASHLFGKLSDANPLKMMSTVGYAAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAE"
BASE COUNT          253 a          219 c          279 g          211 t
ORIGIN      
        1 ccactatcgg ggcgactgtt ggttcagcag taccggtagt ggggacagcc gtcggcgctg
       61 ttgctggtgc agtaattggc ggcattgcag gcgctttgtt ttaaggagtg gtgaatgagc
      121 ggatttgcac aggggttact tgccggattc agcactgttg accaggcaat gacccgtcgt
      181 aaggagcttg gtttgcgaga agcacagctt gctcagcaac agaaaaataa cgagcgcgat
      241 tttgaatttg cgcagtctca gtttgaacat aataaagacg ttgatcagcg gaactttgat
      301 tacagagcca aagttgatga ccgcaattac acactgcagg aaagggagtt taacgccaac
      361 cagaattacc ggaatgcgtc gctggggatg gaacagcaac gactccagtt gcagaaatac
      421 aaccagcgac ggcttgagta taacgatatg ctggcgcgcg atcagcctgt gatggcggcg
      481 ctgggaaaag cgattgatgc gggtgaccat gatgcggctt ctcatctgtt cggtaaatta
      541 tcggacgcca atccgcttaa gatgatgtca acggtaggct atgctgcgaa agcgggtcag
      601 gccgtgaaca acctgcagaa aatctttgat gataagccgg acagggcgat tgcttcgctg
      661 aataccccgg aaaatctcga tgtgctttcc ggcgtgtttg ccccggaact acaacagcgt
      721 attggcatgc ctgattcaac cggggaaaaa acgataaaag aggccaggat tggcagtatc
      781 gtaccggcgc agcaggaagg gtacgtactt attggccttg atctcacata cagcgatggc
      841 tccaccgcgc ataaacctgt aacagaatac ggcagtgcgc accctgatga tcaaaccgtg
      901 ctggcgatac ccgttgataa ggctatcgct caggtcaggg atcgcagcaa atttgcagag
      961 at
//
LOCUS       DAZGAN020000363          946 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZGAN020000363 DAZGAN020000000
VERSION     DAZGAN020000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.370"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..191)
                     /locus_tag="ACHKYO_RS26835"
                     /old_locus_tag="pgaptmp_005367"
     CDS             complement(<1..191)
                     /locus_tag="ACHKYO_RS26835"
                     /old_locus_tag="pgaptmp_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431991.1"
                     /translation="MKPRNINNSLSLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLIHDLSIKIA"
     gene            385..809
                     /gene="yqeK"
                     /locus_tag="ACHKYO_RS26840"
                     /old_locus_tag="pgaptmp_005368"
                     /pseudo
     CDS             385..809
                     /gene="yqeK"
                     /locus_tag="ACHKYO_RS26840"
                     /old_locus_tag="pgaptmp_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            845..925
                     /locus_tag="ACHKYO_RS26845"
                     /old_locus_tag="pgaptmp_005369"
     CDS             845..925
                     /locus_tag="ACHKYO_RS26845"
                     /old_locus_tag="pgaptmp_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431992.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
BASE COUNT          303 a          138 c          166 g          339 t
ORIGIN      
        1 gctatcttta ttgacaaatc atgtattaga tattttcctc caagagattc ttgttcaata
       61 taatttaaac cagaccccat tgtgatttta actggattaa cggatttctc ttcatttttc
      121 ttatttttgt tctcctgatc aggaactaat ggttgcagtg ataggctatt attaatattt
      181 cgtggcttca ttttacattt ctctcatttg attagagcaa aatccgaatt cgcagcttct
      241 gataatgtca aatatcacct tatggagacg ttacgtagaa tgactatatt tgttatcggc
      301 acattagggg tattaatttt agtattgatg gaaggtgatt attgaacagt aacatcgagt
      361 cacggtaaaa atgtgaggta tacaatggac attgagtttt cgcagattca tgaaatggtc
      421 tatatgcatg atattgtaaa ttcggactcg aaaaagaaac caaggattcc gttaaaaaat
      481 tcattaatgc tgagaaggtt ctcacgcaaa caacatcatg gacattaaat tcaagatatg
      541 tgaatgttaa ttctgtcaat aaagtcaacg taaagagtaa agttaaaaac agttatatct
      601 cacgatctgt caatgatgag tttagtttaa cagatgatga aattaattct tttaaagaaa
      661 cgttaatcct ttcctctatt gattcactga gtaaactggt tcttaataat cctctttctg
      721 tattgtttac atcgactgta cgaaggaata ataatcgagc taagatgaat gtcgagttcg
      781 actcatggat ttgcaccaga tgttgttgat acatgatatt ttacattgca aggagatgtc
      841 taaaatgaaa gatgttgatc aaaactttga tgctttagac tgccatatac tgcgagaata
      901 tttaatttta ttgttttatg attaacgagg ttccatcttt ttgtta
//
LOCUS       DAZGAN020000364          917 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZGAN020000364 DAZGAN020000000
VERSION     DAZGAN020000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.371"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            120..866
                     /gene="istB"
                     /locus_tag="ACHKYO_RS26850"
                     /old_locus_tag="pgaptmp_005370"
     CDS             120..866
                     /gene="istB"
                     /locus_tag="ACHKYO_RS26850"
                     /old_locus_tag="pgaptmp_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HGU4431993.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
BASE COUNT          280 a          212 c          227 g          198 t
ORIGIN      
        1 atcctggaaa aaggtctgga taaacaacct gttcaggatc caaaaccaga tctgttatcc
       61 acgatggaac acgaaaacgt acgcggcagt gagtattacc actgatacgg gatccaatga
      121 tgaatcatct ttacgaacaa ctgaccgcac ttaaactcac cggcttccgt gatgcgctta
      181 aaaagcaact tgctcagccg ggcacatacc aggagctggg cttcgaagaa cgcctgtcat
      241 tactgacagc agaagaacta acctgccgtg aaaacaggaa ggcagagcgt ctgatcaaac
      301 atgcacggtt cagacttaat gctgagttat caaagctgga ttatcgtaac aatagagggc
      361 tggacagggc cctcatccgt tcactcagtc agggaaactg gttaaccctg aaacaaaata
      421 ttttactgac cggggccacc ggcagcggta aaacgttcct ggcatgtgca cttggtcata
      481 atgcctgccg acagggatac aaggtctact attatcgcct taaagcgctg atggaacagt
      541 gctatcaggg gcatgctgat ggaagataca gcaaactttt gaccaggctg aataatagcg
      601 atctgctgct tctggatgac tgggggctgg aacctctctc atcagaacag cgtagcgacc
      661 tgctggaaat agtggatctg atgtaccaac gaggctcaat catcgtagtg agccagttgc
      721 cggtggaaaa ctggtacaaa atgatcggag actccacaca tgcggatgcc atcctagatc
      781 gactggttca tggcagtatc aagatcgaac ttaaaggaga atcaatgcgg aaaatacaat
      841 ctccgttgac cgaaggagat cagtgaaggt aatttaaaaa cggttctgtg aaagtgacac
      901 gaaccgatct ccatcga
//
LOCUS       DAZGAN020000365          899 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGAN020000365 DAZGAN020000000
VERSION     DAZGAN020000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.372"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..148)
                     /locus_tag="ACHKYO_RS26855"
                     /old_locus_tag="pgaptmp_005371"
     CDS             complement(<1..148)
                     /locus_tag="ACHKYO_RS26855"
                     /old_locus_tag="pgaptmp_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4431994.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRK"
     gene            263..729
                     /locus_tag="ACHKYO_RS26860"
                     /old_locus_tag="pgaptmp_005372"
                     /pseudo
     CDS             263..729
                     /locus_tag="ACHKYO_RS26860"
                     /old_locus_tag="pgaptmp_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(768..>899)
                     /locus_tag="ACHKYO_RS26865"
                     /old_locus_tag="pgaptmp_005373"
     CDS             complement(768..>899)
                     /locus_tag="ACHKYO_RS26865"
                     /old_locus_tag="pgaptmp_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431995.1"
                     /translation="DAYVDEVLARMVGRSPAENSTENDPQPVEQPEPVQPGTDGTMM"
BASE COUNT          244 a          194 c          201 g          260 t
ORIGIN      
        1 atttccttgt gtatgtgggg tgctctgtgg cgctcggaga tgtgatttga ttgagggttt
       61 tacagcgcgg acattttact tccagataac tgaaactggc ccgtgccagt agcttattgc
      121 agtgtcggca ccgtatgttt cgatacatgg ccgcataacc tcctgctttg ttgttgatgc
      181 ctttctcatg caatcgatcg acaaaaacga tcgattcgat ttgtttaatt gatacaacaa
      241 atagattgaa aagtcatcaa caatgattca ggtaggtgat tatttactta aagaaaacga
      301 agagttaggt ggtgaaaacg atcataaaat gatcatttta ttgacttttt atatagtgaa
      361 ggtaggatcg cgcgtatatg tagtatgttt caaatcaatg ggatactaca tacataaaga
      421 gcgacccaga tagcgcgaac tattctgggc cgatttagac aattttgcat caatgcttta
      481 cttcgagctc gtggcgtatc ctatgatatt ccccagtctc ctgtaaagca gagatgcata
      541 aaaaggagat ctttatcatg gatgaaagag cctgtttgcc ctgcctgtaa gactccctta
      601 cagctcatct atcgccgttc tcgatctcaa aatggcaaga catacaaacc caaaaatgct
      661 tcctgtttca ggatgctgat atgtcctgcc tgttctccca ggagggaaaa tacacaaaac
      721 gtgcattaac aaactcaccc ggcagggaaa cctgccggtc ggtattgtta catcatagta
      781 ccgtcagttc cgggctgaac cggctctggt tgctctacgg gttgaggatc attttccgtg
      841 ctgttttcag ccggagaacg gccaaccatt cgggccagca cttcatcaac atatgcgtc
//
LOCUS       DAZGAN020000366          897 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGAN020000366 DAZGAN020000000
VERSION     DAZGAN020000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.373"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(262..>897)
                     /locus_tag="ACHKYO_RS26870"
                     /old_locus_tag="pgaptmp_005374"
     CDS             complement(262..>897)
                     /locus_tag="ACHKYO_RS26870"
                     /old_locus_tag="pgaptmp_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HGU4431996.1"
                     /translation="EPDVAGAMYLKSEDGQDWYECQALFSPETLKVVYDSRGVITGYG
                     KDTALLWPVNQSVAEVPDTPENRKIDLSGRWGFDGEKITDLLTAEKARGMKGDEINAW
                     RNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFWTDAENNDVEVTA
                     EELIALSEAAGQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRVGWAQE"
BASE COUNT          199 a          290 c          155 g          253 t
ORIGIN      
        1 atttaatttt aatttttagg aaatatctta gtgtattttt tacaccttta ctccaaatac
       61 tggagcacaa ggcagagcaa tactaaataa aaaactgatc tcatgccgct ggatatttca
      121 catctgctta cactgtaatt tctgagcaac atcacaatat catcaaaatt taactgttta
      181 gttcagccat gatatttccc tgcgtaatta ttacgactct ttcagtaaaa acagaaaggg
      241 acaatccctc ttttccacca attactcctg tgcccagcca accctgtatg ccagtatctc
      301 atccgcactg gtcagctttt ccagctcttt cttcatggtg cgctggcgaa cgtggatttc
      361 catccctttg gtgaacatcg cctgccctgc cgcttcactc agcgctataa gctcttctgc
      421 tgtcacctcc acatcattgt tttccgcatc cgtccagaaa aacgcctccg gaagtttccc
      481 cgctttcgct gccgccaccg acggctcaag acgcgtctgc gttgacttcc cgtagtccca
      541 tttacgccca ttgtgctcaa acgtgtagtt cgccgcttcc atcgcattac gccacgcgtt
      601 tatttcatcg cccttcatcc ctcgcgcttt ctccgcggtc agcaggtccg tgattttctc
      661 cccgtcaaaa ccccagcgcc cgctcaggtc aattttcctg ttctccgggg tatccggcac
      721 ttctgccaca ctctggttca ccggccacag taacgcagtg tccttaccgt atcccgtaat
      781 cacaccccgg ctgtcataca ccaccttcag cgtctccggc gaaaacaacg cctgacactc
      841 ataccagtcc tgaccatcct cagacttcag gtacatcgcg cctgcaacat ccggctc
//
LOCUS       DAZGAN020000367          893 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGAN020000367 DAZGAN020000000
VERSION     DAZGAN020000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.374"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..738
                     /locus_tag="ACHKYO_RS26875"
                     /old_locus_tag="pgaptmp_005375"
     CDS             <1..738
                     /locus_tag="ACHKYO_RS26875"
                     /old_locus_tag="pgaptmp_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HGU4431997.1"
                     /translation="AYGYAIVNKTFEGTVAINRDDFEDDNLGIYSPLFQEMGRAAAAQ
                     PDELVFAALRDGINAACYDGQNFFDTEHPVYPKVDGSGDVQMVSNMFVAKTGSVGAQV
                     DYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEFVFGASARRNVGY
                     GFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVPTSLEKQAVQLLE
                     RELFADGNATVSNEMKGKLELVVADYL"
     gene            782..>893
                     /locus_tag="ACHKYO_RS26880"
                     /old_locus_tag="pgaptmp_005376"
     CDS             782..>893
                     /locus_tag="ACHKYO_RS26880"
                     /old_locus_tag="pgaptmp_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4431998.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFR"
BASE COUNT          189 a          230 c          263 g          211 t
ORIGIN      
        1 gcctatggct atgccatcgt gaacaaaact tttgaaggca ccgtcgccat taaccgcgat
       61 gacttcgaag acgataacct cggtatttat tccccgttgt tccaggagat ggggcgcgct
      121 gctgcggcac aaccggatga gctggtcttt gctgctcttc gtgacgggat taatgcggcc
      181 tgctatgacg gccagaactt ctttgatacc gaacatcctg tttaccccaa ggtggatggc
      241 tccggggatg tgcagatggt cagcaatatg tttgtggcaa aaaccggctc tgtcggtgca
      301 caggtggatt acagcggtcc tgcctggtat ctgctcgact gttcacgcgc ggtaaaaccg
      361 ctgatttatc aggatcgccg taaggctgaa ctggttgccc agaccaaagt cgatgaaggt
      421 cgcgcattca ccgataacga atttgtgttt ggtgcatccg cccgtcgcaa tgtgggctat
      481 ggcttctggc agatggccta catgatgcag tcaccgctga cgctggatgc actgtggcac
      541 ggctggtcag ccatgcgtga atttaccgct gacggtggcc gcaagctcgg catcaaaccc
      601 acccatattg ttgtccccac ctcgctggaa aaacaggcgg tgcagctgct ggagcgtgaa
      661 ctgttcgcag acggtaacgc caccgtctct aacgagatga agggcaagct ggagctggtt
      721 gtcgcggatt atctgtaaac ggtgagccgg gcttacggct cggcccctgc ggagggcaaa
      781 tatgaatgaa catcacactg tggcagcaac agatgattca ggtcttcagg tcagtggtga
      841 caattctgtc acggtgctgg cagaagtgcg ttgcagtcgt tcggcgtttc gtc
//
LOCUS       DAZGAN020000368          893 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGAN020000368 DAZGAN020000000
VERSION     DAZGAN020000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.375"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..738
                     /locus_tag="ACHKYO_RS26885"
                     /old_locus_tag="pgaptmp_005377"
     CDS             <1..738
                     /locus_tag="ACHKYO_RS26885"
                     /old_locus_tag="pgaptmp_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HGU4431999.1"
                     /translation="AYGYAIVNKTFEGTVAISRDDFEDDNLGIYSPLFQEMGRAAAVQ
                     PDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNMFVAKTGSVGAQA
                     DYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEFVFGASARRNVGY
                     GFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVPTSLEKQAVQLLE
                     RELFADGNATVSNEMKGKLELVVADYL"
     gene            782..>893
                     /locus_tag="ACHKYO_RS26890"
                     /old_locus_tag="pgaptmp_005378"
     CDS             782..>893
                     /locus_tag="ACHKYO_RS26890"
                     /old_locus_tag="pgaptmp_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432000.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFR"
BASE COUNT          189 a          235 c          267 g          202 t
ORIGIN      
        1 gcctatggct atgccatcgt gaacaaaact tttgaaggca ctgtcgccat tagccgcgat
       61 gacttcgaag acgacaacct cggtatttat tccccgttgt tccaggagat ggggcgcgct
      121 gcggcggtac aaccggatga gctggtcttt gctgccctgc gtgacgggat cagtgcggcc
      181 tgctatgacg gtcagaactt ctttgatacc gaacatccgg tttaccccaa agtggatggc
      241 tccggggatg cgcagatggt cagcaatatg tttgtggcaa aaaccggctc tgtcggcgca
      301 caggctgatt acagcggtcc ggcctggtat ctgcttgact gctcccgcgc ggtaaaaccg
      361 ctgatttatc aggatcgccg taaggctgaa ctggttgccc agaccaaagt cgatgaaggt
      421 cgcgcattca ccgataacga atttgtgttt ggtgcatccg cccgtcgcaa tgtgggctac
      481 ggcttctggc agatggccta catgatgcag tcaccgctga cgctggatgc actgtggcac
      541 ggctggtcag ccatgcgcga atttaccgct gacggtggcc gcaaactcgg cattaaaccc
      601 acccatattg ttgtccccac ctcgctggaa aaacaggcgg tgcagctgct ggagcgtgaa
      661 ctgttcgcag acggaaacgc aaccgtctct aacgagatga agggcaagct ggagctggtt
      721 gtcgcggatt atctgtaaac ggtgagccgg gcttgcggcc cggcccctgc ggagggcaaa
      781 tatgaatgaa catcacactg tggcagcaac agatgattca ggtcttcagg tcagtggtga
      841 caattctgtc acggtgctgg cagaagtgcg ttgcagtcgt tcggcgtttc gtc
//
LOCUS       DAZGAN020000369          889 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGAN020000369 DAZGAN020000000
VERSION     DAZGAN020000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.376"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            274..>889
                     /locus_tag="ACHKYO_RS26895"
                     /old_locus_tag="pgaptmp_005379"
     CDS             274..>889
                     /locus_tag="ACHKYO_RS26895"
                     /old_locus_tag="pgaptmp_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU4432001.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGENRSSRSLEV
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETTSDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQM"
BASE COUNT          249 a          194 c          214 g          232 t
ORIGIN      
        1 agtcaggtga aaagaatgct ggtggagtgg ggaccggtta tcccggaggg gaaggtcgag
       61 atcactatca gtaagtacga aaaagcgagt tgcaaattag caactcggta acggaattga
      121 gcaacaccct aaatttgggt attacctcgt taaagatact gtatttatga acagtgtatc
      181 cttgataact attaaaaatc gcagtaagtt catcctgcat caacgaaaag ggagtgcagt
      241 cccgctcgtg gataaaaatt tgtggagaaa ccaatgaatc agttgcttgt aattgatggc
      301 gtttctgtgc gccagtactt cgaatctaac tactgtctta acgaccttca gaaagctgct
      361 cttcttgccg ctggtgagaa tcgctcctcc cgttcgctgg aagttcacga gtttatgcgt
      421 cgtcctgaaa cgaaggctct tgtggaatta ttggaagaag aaactacggg agattcccgt
      481 agtattcctg tcatcaccat tcaggggcgc aatggtggga cgtatgtctg taaagagctg
      541 gtctatgcat atgcaatgtg gatcagcccg gcattcagct taaaagtgat acgtactttt
      601 gatgcgcttc ataattcatc accagaagaa accacatccg acaaaattaa atccggggtc
      661 attctgcttg aatcagcagc aaagactcta aatctgtcaa actcctcgaa acttggtgca
      721 taccagaaat tatcaaaggt agctggtctt cctgaactta tgccgatcta tgccattgat
      781 gcacctgctg atgcgccaga tggttcaagc cgccctacgc tgtcgctgag tgcactgctg
      841 aagcagtatg gtatccgcct gacggctaat caggcatatc accagatgg
//
LOCUS       DAZGAN020000370          876 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGAN020000370 DAZGAN020000000
VERSION     DAZGAN020000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.377"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            118..363
                     /locus_tag="ACHKYO_RS26900"
                     /old_locus_tag="pgaptmp_005380"
     CDS             118..363
                     /locus_tag="ACHKYO_RS26900"
                     /old_locus_tag="pgaptmp_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013184986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4432002.1"
                     /translation="MSLIEDWSSADIKAALEKRDTNLSRLSRENNLQPRTLGNALRVP
                     YPKGEKIIARAIGVEPQVIWPTRYERRARRKYVGCND"
     gene            344..>876
                     /locus_tag="ACHKYO_RS26905"
                     /old_locus_tag="pgaptmp_005381"
     CDS             344..>876
                     /locus_tag="ACHKYO_RS26905"
                     /old_locus_tag="pgaptmp_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4432003.1"
                     /translation="MWVAMTELISFSDLPSSLAGLHKKAKREAWKTRLKPGVKGKVLE
                     CEIGALPLTVQQAVRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQLM
                     VERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDNLARSQLDECIVA
                     MVETANAKKGNSRALSE"
BASE COUNT          258 a          183 c          234 g          201 t
ORIGIN      
        1 ccggttgata tttactaagt taatatctaa catatcaaac atacactagt gagtaactca
       61 atagagtagc acacatgagt aataaagttt gccatagaaa gtacacaggg ggccattgtg
      121 agtcttattg aagattggtc ttccgccgat ataaaggcag ccttagaaaa acgcgatacc
      181 aatttgtcac gtttatcaag ggaaaataat ttgcagccta ggactttggg taatgctttg
      241 cgagttccct accctaaagg tgaaaagata attgctaggg ctataggtgt agagccacag
      301 gttatttggc ctactagata tgaacgacgt gcgcggagga aatatgtggg ttgcaatgac
      361 tgaattgatt tcattcagtg acctacctag ttctttggca ggtctgcaca aaaaagcaaa
      421 gagggaagcg tggaaaacgc ggctaaaacc aggggtaaaa gggaaagttt tagagtgtga
      481 aataggagct ttgcctttaa ctgttcagca ggcagtacgc gaacgctatg ccctgcaact
      541 gatgacgcaa aaagccgatg aatcaccggc tccggtggtg acaaaggcca gacgctcacc
      601 tgccgtggtt gatgcggtgg aggcatatcg cggatcaccc caactgatgg tcgaacgcct
      661 caatgccctg actgaaaatc agcgccaggt ggctgatgca cgaatcgcga tcgtcagcga
      721 agttctgaaa gtcgcgcaac aacccggttt cagctgcgcg aaggctatcc ggtttatcgt
      781 tgacaacctg gcacgttcac agctggacga gtgcattgtg gcaatggttg agacggcgaa
      841 cgccaaaaag ggaaacagcc gtgcgttgag tgaaat
//
LOCUS       DAZGAN020000371          871 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGAN020000371 DAZGAN020000000
VERSION     DAZGAN020000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.378"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..711
                     /locus_tag="ACHKYO_RS26910"
                     /old_locus_tag="pgaptmp_005382"
     CDS             <1..711
                     /locus_tag="ACHKYO_RS26910"
                     /old_locus_tag="pgaptmp_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4432004.1"
                     /translation="LLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSIT
                     SSGNLPTFSLTPEGRLTARNADISGHISANSGTLNNVTINQNCRILGKLSANQIEGDI
                     VKTVGKAFPRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHNDIYS
                     SCRLMVKCNGRVLFERTALAESQVYSGVIDMPAGRGHMTLEFSVSAWLVNGWYPTASI
                     SDLLVVVMKKSTAGISIS"
BASE COUNT          200 a          210 c          269 g          192 t
ORIGIN      
        1 ctgctgcagg cggaccgtat cgcgatgatt aacccggaga acggcaacac cacgccgctg
       61 tttgtggcgc aggggaatca gctgttcatg aacgatgtgt tcctgaagcg gttgtttgcg
      121 gtgagcatca cctcgtccgg caatctcccg acgttctccc tgacgccgga gggcaggctg
      181 acggcccgca atgcggacat cagcggacat atcagtgcga actcgggcac gctcaataat
      241 gtgaccatta accagaactg ccggatactt ggaaaactgt ctgccaacca gattgaaggc
      301 gatattgtta aaaccgtggg gaaggctttc ccgcgggact cccgcgcacc ggagcggtgg
      361 ccatcgggga ccattaccgt caggatttat gacgatcagc cttttgaccg gcaaattgtg
      421 atcccggcag tggctttcag cggcgctaaa catgagagag agcataacga tatttattca
      481 tcatgccggc tgatggtgaa atgtaacggc agggtgcttt ttgaaaggac ggcgctggct
      541 gagtctcagg tgtattcagg ggtgattgat atgcctgcag gaagggggca tatgacgctg
      601 gagttttctg tgtctgcctg gctggtgaat ggatggtacc cgacagcgag tatcagcgat
      661 ctgctggttg tcgtgatgaa gaagtccacg gcgggtatca gtatttcgtg atcatgagga
      721 gccggattgc cggaaggaga caaaaaccgt acattatgcg cgggtgcctt tggctgatgg
      781 ccggagggag cacctgaagg ccggatgtga aaaggccccg gacaaacatt catgtttaac
      841 ccgaggcctg acgttcctac cttcaacaag t
//
LOCUS       DAZGAN020000372          860 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGAN020000372 DAZGAN020000000
VERSION     DAZGAN020000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.379"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>860
                     /locus_tag="ACHKYO_RS26915"
                     /old_locus_tag="pgaptmp_005383"
     CDS             <1..>860
                     /locus_tag="ACHKYO_RS26915"
                     /old_locus_tag="pgaptmp_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HGU4432005.1"
                     /translation="LALIPALSLSVFSAGVYAADASGINGTSYYGVTVDQLKGHEYKN
                     LIDFWKDLGFSGKAYLDGKVVNAGTKQPDSVNIQNTAGKYLKYSLFDYGESGLTNTEL
                     RAPRSNEIVSYYDYANETAYFVNTKTNQILFSADGEGVVTTVGADGKTYSRHVPEISE
                     YIYEIKMKSVNPLDSTIDTGLSAADVTDIHARLSSLGTGKTQVGGGATAGGENATAVG
                     YNAIANHTNSVAVGANSQTTRPDEVNVGNRVIGGLRDGVQAADAATYGQLERYRIAEE
                     NARIQGDAQTL"
BASE COUNT          261 a          164 c          210 g          225 t
ORIGIN      
        1 ttggctttaa taccagcatt aagcctgtcg gtattttctg ctggcgtata tgcagcagac
       61 gcctctggaa ttaatggcac ttcatattat ggagtgactg ttgatcagtt gaaaggacat
      121 gaatataaaa atttaattga tttttggaaa gacctcggct tctcaggaaa agcatatctg
      181 gatggaaaag tggtaaatgc aggcacaaaa caaccagata gtgtaaatat acaaaatacg
      241 gcagggaaat atttaaaata ttctctgttt gattacggcg agtccgggtt aacaaataca
      301 gaattacggg caccgagaag taatgaaatt gtctcatatt atgactatgc gaatgagaca
      361 gcttattttg taaacacaaa aaccaatcaa attctcttta gtgcagatgg cgaaggcgtg
      421 gtcacgacag tgggcgctga tggaaaaacg tattcaagac atgttcctga aatcagtgaa
      481 tatatttatg aaattaaaat gaagtctgta aacccgctag actccactat tgacactggt
      541 ctttctgctg cagatgtaac agatattcac gcccgtctgt cttctctggg cacaggtaaa
      601 actcaggttg gtggcggggc aacagcaggc ggagaaaacg ccactgctgt cggttacaac
      661 gccattgcaa accatacgaa cagtgttgct gtaggggcta actctcagac cactcgcccc
      721 gatgaagtaa atgtggggaa tcgtgtaatt ggtgggcttc gtgatggtgt tcaggctgcg
      781 gatgccgcaa cctatggtca gttagagcgc tatcgtattg cagaagagaa tgcccgtatt
      841 cagggtgatg cacagacact
//
LOCUS       DAZGAN020000373          859 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGAN020000373 DAZGAN020000000
VERSION     DAZGAN020000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.380"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>859
                     /locus_tag="ACHKYO_RS26920"
                     /old_locus_tag="pgaptmp_005384"
     CDS             <1..>859
                     /locus_tag="ACHKYO_RS26920"
                     /old_locus_tag="pgaptmp_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432006.1"
                     /translation="TGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIAD
                     ADPMEQPAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELT
                     ERPRLPAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAPE
                     NAAGRQGKILEGETVRRGLPSPDAQNVTAPVREGLPAPDIARNVRMPQPESLPRTVRD
                     SLPELAQQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGE
                     EIKGAAKHRY"
BASE COUNT          209 a          227 c          267 g          156 t
ORIGIN      
        1 accggcctga aaccgtccgt agtgaagaaa ggcgagaatc agtatgccgt tgaaatggat
       61 aatcctgcgt tctctgaaga tgtggcaacg gaaaccatta acaccctggc tgacggagag
      121 cgtattgctg atgctgaccc gatggagcag cccgcgttca tgcgtgaccc gcgattccgt
      181 ggtttcacgg gggatgatac ggaggtacag gcccgccttg cccgtggcaa tgcgccgacg
      241 gcggaggagc ttgtacgttc acagatggct gaaggtgatg ccggtccgac agcacaggag
      301 ttaactgagc gtccacgcct gcccgctccc ggcgatattc atcccggaca gggatatccg
      361 ttaccgggag aggtggcgcg tacgccggat gaaaatcagg ccggacgtgg tggtcgtttt
      421 accacaaccg gtgaggttaa gggccagagt ttccagaaag gacaagctcc ggcaccggaa
      481 aacgccgctg gtcgccaggg gaaaatactg gagggcgaaa cagttcgtcg tggtctgccg
      541 tcaccggatg cgcagaacgt gacagctccg gtacgtgaag ggttaccggc tcctgacatt
      601 gcgcgtaatg ttcgtatgcc tcagcctgaa tcacttcccc gcactgtacg ggactcactg
      661 cctgagctcg cacagcaggc agaagtacgc cgacaggccg gaggaaatcg tgacatcccg
      721 cagcctgaga caatcgcacc tgaatctgaa acaactgtct ctactgacag ggaagctacc
      781 gtgcgcggag gtgaagtcag gggcaaaaaa attgaagact ttggcgagga aattaaaggg
      841 gcagccaaac accgttatg
//
LOCUS       DAZGAN020000374          854 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGAN020000374 DAZGAN020000000
VERSION     DAZGAN020000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.381"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>854
                     /locus_tag="ACHKYO_RS26925"
                     /old_locus_tag="pgaptmp_005385"
     CDS             <1..>854
                     /locus_tag="ACHKYO_RS26925"
                     /old_locus_tag="pgaptmp_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4432007.1"
                     /translation="ADDVLKVTEAISTGLKISGASAAEAGSVITQFSQALAQGVLRGE
                     EFNSVNESGDRIIRALAAGMGVARKDLKAMADDGQLTADKVVPALISQLGILRDEYAA
                     MPETVSDGITKVENAFMAWVGGANEASGATKTLSGALNGVAGNIDTVATAAGVLVAVG
                     VARYFGNLASGAMSATAGLVTAARNEVALAEAQLRGTQIATARARAAVYRAQQAVAAA
                     RGTEMQIAAEARLAVTQERLNRNIAARTAAQNALNSTTAVGSRLMSGALGLVGGVPGL
                     VMLGAAAW"
BASE COUNT          174 a          206 c          317 g          157 t
ORIGIN      
        1 gctgatgatg tgctgaaggt gacggaggcc atttcgacag ggctgaaaat ttccggtgcc
       61 agtgcggcag aggcgggttc ggtgatcacc cagttcagcc aggcgctggc acagggggtg
      121 ttgcgcggcg aggaatttaa ttcggtcaat gaaagtgggg accggatcat tcgcgcactg
      181 gctgcaggca tgggcgtggc ccgtaaggat ctgaaggcga tggcggacga tggtcaactg
      241 acggcggata aagtcgttcc cgcgttaatc agccagctgg ggatattgcg tgatgaatat
      301 gcagccatgc cggaaacggt gtctgacggg atcacgaagg tggaaaacgc ctttatggcc
      361 tgggtgggcg gcgcgaatga ggccagcgga gcgacgaaaa cgctctccgg cgcactgaac
      421 ggtgtggccg gaaatattga taccgtggca acagctgcgg gtgtgctggt tgctgtcggg
      481 gtggcccggt actttggtaa tctggcttcc ggagcgatgt ctgccacggc aggacttgtg
      541 accgctgcac gtaatgaagt ggcactggct gaggcccagt taaggggaac gcagattgcc
      601 acggcgcggg caagggcagc cgtgtaccgg gctcagcagg ctgtggcggc agcccgcggg
      661 actgagatgc agattgcggc agaggcccgt ctggcggtca cacaggaacg cctgaacaga
      721 aatattgctg ccagaaccgc cgcccagaat gcgctgaaca gtacaacggc ggtgggctca
      781 cgtctgatga gcggtgcgct gggactggtt ggaggcgtac ccggactggt gatgctggga
      841 gctgccgcat ggta
//
LOCUS       DAZGAN020000375          843 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGAN020000375 DAZGAN020000000
VERSION     DAZGAN020000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.382"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            40..669
                     /locus_tag="ACHKYO_RS26930"
                     /old_locus_tag="pgaptmp_005386"
     CDS             40..669
                     /locus_tag="ACHKYO_RS26930"
                     /old_locus_tag="pgaptmp_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGU4432008.1"
                     /translation="MSIEQIINEINVYLNSLKKEDERIDAINKIKIELHRSSPFYAEP
                     VDCVIWIKNNLIEANDYNPNVMAPSEKRLLTHSLLVDGFTQPVVTLAQKGKYTVIDGF
                     HRQMLGKSTTKLEKKLKGYLPVTCIKHDNNFKAYSIASTIRHNRARGKHQIDALSDIV
                     RDLSRMGWDDLRIGSELGMDTDEVLRLKQISGLTELFEEENFSPAWTIK"
BASE COUNT          325 a          149 c          157 g          212 t
ORIGIN      
        1 aaacaagaaa aatatacaaa ttaaaagaaa tagaaacaca tgagcattga acaaatcata
       61 aatgaaataa atgtatacct taattctctt aagaaagaag atgaacgcat tgatgcaatt
      121 aataaaataa agattgagtt acataggagt agtccttttt acgcagaacc tgttgattgt
      181 gttatatgga taaaaaacaa tctaattgaa gcaaacgact ataatcctaa cgtcatggct
      241 ccctccgaaa aaaggttatt aacacactcc ctgttggttg atggattcac tcaacctgtt
      301 gtcaccttag ctcaaaaggg gaaatacacc gttattgacg gatttcacag acaaatgctt
      361 gggaaaagca caacaaaact agagaaaaaa ctaaaaggat atttacctgt cacatgcatt
      421 aagcatgata ataactttaa agcttactct atcgcctcga ccatcaggca taacagggcc
      481 agaggaaagc accagataga cgcgctctcc gatatcgtcc gagatctttc ccgaatggga
      541 tgggatgatc tccgaatagg tagtgaactg ggcatggata cagatgaggt actgagatta
      601 aaacaaataa gtgggttaac agaactattt gaggaggaaa actttagtcc ggcatggaca
      661 ataaagtaat catttagggg gtgactttat ccagtaatta ccgactgata tatgatcacc
      721 ccatgtaatg gacagctatt gacagatcaa aaacatgggg gatattaatt caactctgaa
      781 tactcgctaa atacccatct tagattatca aaaaacgaat gattacatat cagcaataat
      841 aaa
//
LOCUS       DAZGAN020000376          842 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZGAN020000376 DAZGAN020000000
VERSION     DAZGAN020000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.383"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(104..841)
                     /locus_tag="ACHKYO_RS26935"
                     /old_locus_tag="pgaptmp_005387"
     CDS             complement(104..841)
                     /locus_tag="ACHKYO_RS26935"
                     /old_locus_tag="pgaptmp_005387"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU4432009.1"
                     /translation="MQSIDFSNCNLIIAHQFKNKISSQAKTFIQKLLHENNNISYYQL
                     NSFGVTKSRNFLINKAKSNYIAFCDDDIYYFPNAISTVLDKMEHENIDVATCMTITDD
                     SKPLKNYASRDYHHNKRTILRVGTVEIICKRESILKTDLFPEDMGAGALFPLCDEPVF
                     LNNCLKQSLKVQFLSIPLCYHPNVSSGSNLNRCYTIARGVCFRRIYGMPALLLILIFS
                     LKMKLKYRKLDLLTYTKSLYRGYNEGK"
BASE COUNT          301 a          144 c          123 g          274 t
ORIGIN      
        1 aataaaggat actggtatcg caggccactt ccatgaaaag aaaggccaaa gtgacaaaaa
       61 gcacagaaat ccatagaacc aattaagata tggtgtcaaa acgctacttg ccttcattat
      121 acccccgata aagagatttg gtataagtta gcaagtctaa tttcctgtac ttcagtttca
      181 ttttcaaaga aaatattaag atcaatagca atgcaggcat gccatatata cgtctgaaac
      241 aaacacctct tgcaatggta tagcatctat ttaaattact acctgaagaa acattaggat
      301 gataacaaag aggtatactt aaaaactgaa ccttgagtga ttgttttaaa caattattta
      361 aaaaaacagg ctcatcacac agaggaaata aagcaccagc ccccatgtcc tctggaaata
      421 aatcagtctt caaaatactt tcacgtttac agattatctc tactgtacca actcttaata
      481 ttgttctttt attatggtga tagtcccttg atgcataatt tttcaaaggt ttgctatcat
      541 cagtaatagt catacaagtc gcgacatcaa tattttcatg ttccatctta tctaagacag
      601 ttgaaattgc attcgggaaa taatatatat catcatcgca aaatgcgata taattacttt
      661 ttgctttgtt tatcaaaaaa tttctgcttt tagtcacgcc aaaactattt aactgataat
      721 atgatatatt gttattttcg tgcaaaagct tttgaataaa ggtctttgct tgagatgata
      781 ttttgttttt aaattgatga gcaattatta aattgcaatt actaaaatca atcgactgaa
      841 tt
//
LOCUS       DAZGAN020000377          825 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGAN020000377 DAZGAN020000000
VERSION     DAZGAN020000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.384"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(30..239)
                     /locus_tag="ACHKYO_RS26940"
                     /old_locus_tag="pgaptmp_005388"
     CDS             complement(30..239)
                     /locus_tag="ACHKYO_RS26940"
                     /old_locus_tag="pgaptmp_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HGU4432010.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEHALSFAAQS"
     gene            complement(335..709)
                     /locus_tag="ACHKYO_RS26945"
                     /old_locus_tag="pgaptmp_005389"
     CDS             complement(335..709)
                     /locus_tag="ACHKYO_RS26945"
                     /old_locus_tag="pgaptmp_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU4432011.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESVRKK"
     gene            complement(724..>825)
                     /locus_tag="ACHKYO_RS26950"
                     /old_locus_tag="pgaptmp_005390"
     CDS             complement(724..>825)
                     /locus_tag="ACHKYO_RS26950"
                     /old_locus_tag="pgaptmp_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4432012.1"
                     /translation="DAGKYTCVVTDSAEKAQSVTSVECTVTVSAAAG"
BASE COUNT          175 a          290 c          183 g          177 t
ORIGIN      
        1 aaaaaaaccg cctcagcggt tctcatcatt catgactgtg ctgcaaagct cagcgcgtgt
       61 tccagcgccg caaacggatc cacctccggc ttatcctcat cctcgcccca gcagagcatg
      121 gcgtccttca gtgcaacatt catcccctgt gccccgaaaa ccgctttcac gatctgtgca
      181 ttacggatat ccccgcgctc atcacccagc ggggatatcc tgtcgaactc catccacatc
      241 atcgcctcgc tcgcactcag gctgtgccgc agttcggata aggtgcgccc cagacggagc
      301 gcaagtcgca tcagaaagcg aatttccggg cgggctactt ttttctgacc gactctgcat
      361 cagcgatcag ttccagtgcc tgacgcagca agcgggcatg taccggacca tagacggcca
      421 gcacctgctc acggtcgtcc ggagtgaaca cccgttgcag atccgtatca cacaggacat
      481 cgcagaacag cgtcacatcc gcttccaggt tacggcgggt tttcgccacc accgacaggg
      541 tatcatcatc ctctccatca ccattgagca cgtcctgcca cagataccag gcctctgccg
      601 aaggctcccg cagcaccacg ctgacattac cccattccgg caccttcacc gttttatgac
      661 gaaaccctga cagtctggcc agcgccagcg ttttcagatc ctttttcatg atgacccatc
      721 cccttatccg gcggctgcgc tcactgtcac ggtgcattca acagacgtca cactctgtgc
      781 tttctctgcc gaatcagtca ccacgcaggt atatttcccc gcatc
//
LOCUS       DAZGAN020000378          825 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGAN020000378 DAZGAN020000000
VERSION     DAZGAN020000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.385"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(69..269)
                     /locus_tag="ACHKYO_RS26955"
                     /old_locus_tag="pgaptmp_005391"
     CDS             complement(69..269)
                     /locus_tag="ACHKYO_RS26955"
                     /old_locus_tag="pgaptmp_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4432013.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            311..676
                     /locus_tag="ACHKYO_RS26960"
                     /old_locus_tag="pgaptmp_005392"
     CDS             311..676
                     /locus_tag="ACHKYO_RS26960"
                     /old_locus_tag="pgaptmp_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4432014.1"
                     /translation="MAQLRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSVREAASGMPD
                     PTLPEVSGGSGRADDIIGL"
BASE COUNT          178 a          196 c          229 g          222 t
ORIGIN      
        1 tgttccgtcg gatatgaaga tgaaatattg ttggaggaca gtgggtacct gctcctgtaa
       61 ccgaacgttc atttctcgtt atttgtcatg ctggccgggc gcagatgcgt tgcatctgtt
      121 gccagccttc tcctgcaggc ttcaataacc cacgctgaaa agttaccgga ccctttatgc
      181 tcaagggcga tgttgatctg ttcaatcatg tgattgggga aacggatatt gcgggttgtg
      241 gttctgcggg tccggttttt cgatgacata tttatttcct ttactgattg ccatatgacg
      301 gggattttac atggctcagc ttcgtacact ccagagcaga atcaaaacac tgaatacccg
      361 acgggtgaat attctgaagg gtgaacagcg tcgtgtcagt ggcagtgcac gtgtttccct
      421 caagcgtcat atctggctca gggacgccgg gcagtgctgt ctctgtggtc gtgtggttga
      481 cctctgtgac agtgaactcg atcaccgaat tgcacttcag ttcggtggtg gtaatgagga
      541 gacgaatctc tggacgctct gtaccgaatg ccatcgacaa aagtctgttc gtgaagcggc
      601 gagtggtatg ccggacccga cactgccgga ggtgtccgga ggtagtggca gagcggacga
      661 catcatcgga ctgtaacccg acccgggggg gtatcatccg gcgtaaaaaa ctatcgctcc
      721 ggacaccgcg ccccctctca cgcagagaaa aaattcccgt ttcagggcag ttaacatgtt
      781 aactggctgc ccgggcattt ttgcggtttt tatctttatt attca
//
LOCUS       DAZGAN020000379          823 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGAN020000379 DAZGAN020000000
VERSION     DAZGAN020000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.386"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(22..366)
                     /locus_tag="ACHKYO_RS26965"
                     /old_locus_tag="pgaptmp_005393"
     CDS             complement(22..366)
                     /locus_tag="ACHKYO_RS26965"
                     /old_locus_tag="pgaptmp_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HGU4432015.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVAVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(363..809)
                     /locus_tag="ACHKYO_RS26970"
                     /old_locus_tag="pgaptmp_005394"
     CDS             complement(363..809)
                     /locus_tag="ACHKYO_RS26970"
                     /old_locus_tag="pgaptmp_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HGU4432016.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
BASE COUNT          182 a          281 c          200 g          160 t
ORIGIN      
        1 ttttgtagaa aaaagaaaaa gtcacaccgt gacctgaaac tccagggttg cacggtaaca
       61 gcggttttcc ggaatatagt cctgcatttc actgacggat cccggggcca gcagcattat
      121 ggcttcacgg gcgtcctgac gtatctgacg cgcctgcgtc acagtcccgg cataaacgtc
      181 tatctgcacc gccactgagg actccgcctg cccgcccatc acgtccgcag acaccgatga
      241 aatcaggctg aaaaccaccc acggaagcgc caccgacggc ctgccatcca gcagggggac
      301 cacatacggg tacacctgcc cgccggcaag atgcgccaga tgaggataca aatccgcctc
      361 cgtcatcgtc tcagtacctc atcaatggcc cggttcatcc gcgcaatcgc cacctgcgct
      421 gcctgttcac tgcgcacatc aaacgccggg cgcacaaacg ggtgcggtgg catattcacg
      481 gtccccattt ccacaaaccg ccagtagaaa gcattgcgcg ggttatccgc cttcatggtg
      541 ttatcgctgt taccggtgtc cggattaaca ccacggatat ggacaccgga ttccatcccg
      601 ccatcgcggg agcgccggga aaggaccacc acattgcggc gcagttttcc cctgcgcacc
      661 ggtgcccgtg acaccacttc ttctttcagc acattcgcac ccgcacgggt tgcctcacgc
      721 agcacccggt tattttccgc accactcaga agctgcaaat cgcggctgat gtcctccagc
      781 cccgaaaaat ccagcagggt ttcgatcatt tttcccctcc cag
//
LOCUS       DAZGAN020000380          821 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZGAN020000380 DAZGAN020000000
VERSION     DAZGAN020000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.387"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..387)
                     /gene="tli1"
                     /locus_tag="ACHKYO_RS26975"
                     /old_locus_tag="pgaptmp_005395"
     CDS             complement(<1..387)
                     /gene="tli1"
                     /locus_tag="ACHKYO_RS26975"
                     /old_locus_tag="pgaptmp_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HGU4432017.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKNLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYKTTYQYLDNTEPSQVSVGRWSDHLGATESYYNK
                     GKALPLMLRFCWDSIIDKKSYETLIFF"
     gene            complement(531..>821)
                     /locus_tag="ACHKYO_RS26980"
                     /old_locus_tag="pgaptmp_005396"
     CDS             complement(531..>821)
                     /locus_tag="ACHKYO_RS26980"
                     /old_locus_tag="pgaptmp_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HGU4432018.1"
                     /translation="KKGTWEQMVTPYTDNRWNETYYRSTMIIGLAPEGKVRVWLGDRG
                     NPVVPQLDAKITTVSGDKMKMCKGVTKHPDGYVYYGDTPDFIKGKIYPYGNW"
BASE COUNT          226 a          200 c          137 g          258 t
ORIGIN      
        1 aaaaaaaatc agagtttcat aactcttttt atcaattatt gaatcccagc aaaaacgcaa
       61 catcagaggt aatgctttcc ccttgttata atacgactca gtcgcgccca gatggtcact
      121 ccagcgccct acactgacct gactcggctc ggtattatca agatactgat aagtcgtctt
      181 ataaccttcc ccgtccacca tcaatgcctg agtggccagc gcaggcagag cctttggata
      241 aataaagaga aatttccatt catcataggg taaattttta taaccccatt cagtcttatc
      301 cgaatcagca tactgtgagc taaccgaatt acacccagtt aacaatgcca gtaatggtgc
      361 gataaaaatg attttatctc tggtcatgta ttgtcatcct gtagcgttaa acaccagaca
      421 tcataacttt agtatttttt actgcgttat cattcagcta ttgcccgttt ttttacgcca
      481 gatgaaaatt agattttctt tttatatgct catcattatt ccggtttgag tcaccagttg
      541 ccgtaggggt aaattttccc tttgataaaa tctggtgtat caccgtaata aacatatcca
      601 tctggatgtt tagtaacccc cttacacatc ttcattttat cacccgatac tgtagtaatt
      661 ttagcatcca actgaggtac caccggatta ccgcgatctc ccagccagac tcttaccttc
      721 ccttcaggtg ccagaccaat aatcatcgtt gaacgataat aggtttcgtt ccagcggttg
      781 tctgtgtaag gcgtaaccat ctgctcccag gtcccttttt t
//
LOCUS       DAZGAN020000381          820 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGAN020000381 DAZGAN020000000
VERSION     DAZGAN020000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.388"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..633
                     /locus_tag="ACHKYO_RS26985"
                     /old_locus_tag="pgaptmp_005397"
     CDS             <1..633
                     /locus_tag="ACHKYO_RS26985"
                     /old_locus_tag="pgaptmp_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HGU4432019.1"
                     /translation="LSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPEL
                     GTAALSALTGIKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNAL
                     RTEYVALSAQHGSTTLEQLLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQP
                     VAALTGLQTDTVTVPDKKTATAVLSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            633..>820
                     /locus_tag="ACHKYO_RS26990"
                     /old_locus_tag="pgaptmp_005398"
     CDS             633..>820
                     /locus_tag="ACHKYO_RS26990"
                     /old_locus_tag="pgaptmp_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HGU4432020.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGS"
BASE COUNT          193 a          244 c          233 g          150 t
ORIGIN      
        1 ctgtcggcgg atgaccttta taacgcctta aaccctcctt taaaggatac ctccatgaat
       61 gagcaattac gccagttgct gacggcgctc ggtctgaccg tggctgacgg tgacgaattt
      121 acgccggagc tgggcacggc agcgctgtct gccctcaccg ggatcaaaac ccgtgcggat
      181 gcacacgaca acctgaaaac acaggtcgcc agtctgtcgg cagagctgga aactgcaaaa
      241 ggcacaccgg ctggcggcag tattgacctg acgaaatacg tgcccgttga aacgtataac
      301 gccctgcgta ccgaatacgt tgcgctgtcg gcgcagcacg gcagcaccac gctggagcag
      361 ttgctggaca aggccgagtc cgaaggacgc atcttcaaaa gtgaacgcgg ctatctggaa
      421 caactgggcg ggcagattgg cgtggcggca ctttctgcac agcttgacgc ccgccagccg
      481 gttgcggccc tgaccggcct tcagaccgac accgtgaccg tgccggataa aaagactgcc
      541 accgctgtgc tgtcggctga agatatcgcg gccgccaaac tgctgggtaa aaccgaagcc
      601 gagttcctga aaatgaaaga ggaaatgcaa taatgcctac cccgattaca ccggcgatga
      661 tcaccgccct gatgacgggc taccgttctg attttcaggc cgggatgtcc atggccccgt
      721 cgcagtacaa aaaaattgcg atgaccgtgc cctccacatc gaaatccaac acctacggct
      781 ggctggggca gttcccgcag ttccgtgagt ggatcggctc
//
LOCUS       DAZGAN020000382          816 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGAN020000382 DAZGAN020000000
VERSION     DAZGAN020000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.389"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>816
                     /locus_tag="ACHKYO_RS26995"
                     /old_locus_tag="pgaptmp_005399"
     CDS             <1..>816
                     /locus_tag="ACHKYO_RS26995"
                     /old_locus_tag="pgaptmp_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU4432021.1"
                     /translation="PSGRPDATAIALLSEIVYWYRPTEVRDEHTGALLGYRKRFQGDK
                     LQRSYQAFAEQFGFGKRETADALKRLRDAGFITLDLRTVEMLDGVKCSNILFVGINPQ
                     AIAAITTPSSVSPESNSNNAISDTAITLKRNTPRRHNGTGDTPNVDTNTEITTEITTE
                     TKNTIDASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARLNEGVN
                     PETMLEGVKRYAGWVSAMGNSGTQFVKQAVTFFGPDRHFEESWEVPAVSAARR"
BASE COUNT          219 a          198 c          225 g          174 t
ORIGIN      
        1 acccagtggg cgtccggatg cgacggctat cgctctgctt tcagagatcg tttactggta
       61 ccgcccgaca gaggtcaggg atgagcacac cggagcgttg ctgggatatc gcaagcgttt
      121 tcagggcgac aaactgcaaa gaagctacca ggcgtttgct gagcagtttg gtttcgggaa
      181 aagggaaacc gcagatgcgc tgaagcgtct gcgcgatgca gggtttatta ctctggattt
      241 acgcacggtg gaaatgctcg atggggtgaa atgcagcaat attttgtttg tcgggatcaa
      301 cccacaggca attgcggcca tcaccacacc ttcttctgtt tcgccagaaa gtaacagcaa
      361 taatgcaatc agcgatacag ctattacgtt aaaacggaac accccccgac gtcataacgg
      421 aacaggggat acgccgaatg ttgatacaaa tacagagatt actacagaga ttacaacgga
      481 gactaaaaac actattgatg catccgctga cgcgtctgcg ccagcgcgtt ctgcccgaca
      541 ggaatattca ccggaatttg aacaggcctg gcaggaatat cccaaacgtg ctggtggcaa
      601 ttcaaaatca gcagccttca aagcctggaa agcccgtttg aacgaggggg taaaccccga
      661 aaccatgctg gaaggtgtga aacgctacgc gggctgggta tctgcgatgg gcaatagcgg
      721 cacacaattt gtgaaacagg ctgtcacgtt ctttggtccg gatcgtcatt tcgaagaatc
      781 ctgggaagtt cctgcggtat ctgcagccag acgcga
//
LOCUS       DAZGAN020000383          808 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZGAN020000383 DAZGAN020000000
VERSION     DAZGAN020000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.390"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            343..>808
                     /gene="kgtP"
                     /locus_tag="ACHKYO_RS27000"
                     /old_locus_tag="pgaptmp_005400"
     CDS             343..>808
                     /gene="kgtP"
                     /locus_tag="ACHKYO_RS27000"
                     /old_locus_tag="pgaptmp_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HGU4432022.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVE"
BASE COUNT          209 a          194 c          187 g          218 t
ORIGIN      
        1 agagtaggga actgccaggc atcaaattaa gcgaaaggcc atccgtcagg atggcctttt
       61 tgcgttggtg aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc acatatgcca
      121 gattcagcag cgaatacggc ttccccaact tgcccacctc catacgtgtt ctcctcacca
      181 gaaatttatc cttaagctcc tcaataacca ttttcctgct aactaaattc atggttaagg
      241 ttgcataatg atacgcaaca aatgtataac attttcttta ccaaaagaaa taaacaaaag
      301 cgaccgacaa aagcatcgga ttacggcagg agacataatg gcatggctga aagtactgta
      361 acggcagaca gcaaactgac aagtagtgat acacgtcgcc gcatttgggc gattgtgggg
      421 gcctcttcag gtaatctggt cgagtggttc gatttctatg tctactcgtt ctgttcactc
      481 tacttcgccc acattttctt cccttccggg aacacgacga ctcaactact acaaacagca
      541 ggtgtttttg ctgcgggatt cctgatgcgc ccaataggcg gttggctatt tggccgcata
      601 gccgataaac atggtcgcaa aaaatcgatg ctgttatcgg tgtgtatgat gtgtttcgga
      661 tcgctggtta tcgcctgcct cccaggttat gaaactatag gtacgtgggc tccggcatta
      721 ttgcttctcg ctcgtttatt tcagggatta tccgttggcg gagaatatgg caccagcgcc
      781 acctatatga gtgaagttgc cgttgaag
//
LOCUS       DAZGAN020000384          799 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZGAN020000384 DAZGAN020000000
VERSION     DAZGAN020000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.391"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            206..664
                     /locus_tag="ACHKYO_RS27005"
                     /old_locus_tag="pgaptmp_005401"
     CDS             206..664
                     /locus_tag="ACHKYO_RS27005"
                     /old_locus_tag="pgaptmp_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432023.1"
                     /translation="MKIYWTRKNIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILFKVYDFLLPAQDTFPSDIIRSLCVVCPSILIWFQFSVYVMRKYYRHTLER
                     GKETETISERLIREADTREYELWRPAHRFFSIIFLIILLVCIHSLITTIK"
BASE COUNT          287 a          133 c          123 g          256 t
ORIGIN      
        1 caaacacact cttttcatta aataaatcac ctgattaaac catgaaagtg tgatgtaagt
       61 caaaattagt gacacaatca attgataatg attatctcta tcattatcgt gaaattcgag
      121 aatgaaagga gaagtagtca ttataagact tttataaatc gtcatcatca tagtaaagta
      181 gaacactttt tatatgagtt actatatgaa aatatactgg acaagaaaaa acattcctga
      241 actttcagca cttccacctt cattaagaaa gaaaaatttt actgacgcat ataatgctgc
      301 atcttcccat atcgaatact ggataggtgc tggcgtctca tttatctcga tgatgatttt
      361 attcaaagta tatgactttc ttttacccgc ccaagatact ttcccgagtg atatcataag
      421 aagtttatgt gttgtgtgtc catccatatt aatctggttt cagttttcgg tatatgtcat
      481 gaggaaatat tacagacata ctctggaacg aggtaaggaa accgaaacca tttcagaaag
      541 gttgatacgt gaggctgata cacgagaata tgaactatgg cgtccggccc atcgcttttt
      601 ctccattatt tttctcatta ttctattagt ttgcattcat tcactgataa caactataaa
      661 ataaaagtta tggctattct gatacaggaa aataaacgtt cattctatgg ttacctcttc
      721 ctgttatata aaagaatttt aaacgacaag taagggaata taaatgaaaa taacaaaaaa
      781 taaaaatgca actataaaa
//
LOCUS       DAZGAN020000385          794 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZGAN020000385 DAZGAN020000000
VERSION     DAZGAN020000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.392"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..226
                     /locus_tag="ACHKYO_RS27010"
                     /old_locus_tag="pgaptmp_005402"
     CDS             <1..226
                     /locus_tag="ACHKYO_RS27010"
                     /old_locus_tag="pgaptmp_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432024.1"
                     /translation="RNNATLYEVYHEVKNVNKGVGLVGDNGIVLVIRSSNSKNTLEEF
                     HEFFGNFKPNNSTARVEVIDQSVLEPFWRF"
BASE COUNT          293 a          118 c          134 g          249 t
ORIGIN      
        1 tagaaataat gctacattat atgaggtata tcatgaggta aaaaatgtta acaagggagt
       61 tggcctggtg ggagataatg gtatcgtcct ggtaattaga agttccaatt ccaaaaatac
      121 acttgaggaa tttcatgaat tctttggaaa cttcaaacct aataattcaa cagctcgagt
      181 tgaagtgatt gatcaatccg ttttagaacc tttttggaga ttttaaaaat atgatcacca
      241 aatcaagtat actcatagag tataaagata aaagtaaact aaccgatagt atttataagt
      301 ttgtaaatgc tctagacatt gacttttcga tattaaaata tgactacttt gatgtaaatg
      361 agtataaaaa aacattactt atgacacatc ttttattgac aaagatgcag aatatattga
      421 attactagcg aagattgatt tttcttatga agttgatttc ggcacatcaa caacatcgat
      481 tgaaagcaag tatcttccat ctctaactga tttcatcgct caatcactat cacaacaatt
      541 aaattgtgac ataataacca gctttaaacc attaagtgga gatgagtttt gcccaataac
      601 atatttttca aaaggtaaag aagttatcaa ttttacgaca tttaattcaa attgttggga
      661 tggggtatgc tgggtaaaaa aaaggaggca aacaaatatc cgctaacgta gtttttcaat
      721 agctacacca tgtcacgtaa catgatgcct ggatattaac cagggaaaga tcatcagaag
      781 tacacttttt gtac
//
LOCUS       DAZGAN020000386          787 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZGAN020000386 DAZGAN020000000
VERSION     DAZGAN020000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.393"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(134..>787)
                     /locus_tag="ACHKYO_RS27015"
                     /old_locus_tag="pgaptmp_005403"
     CDS             complement(134..>787)
                     /locus_tag="ACHKYO_RS27015"
                     /old_locus_tag="pgaptmp_005403"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU4432025.1"
                     /translation="IEELEAYLIAAGLSDYQLTEKDYLILEHFKNIEFSGFPVTELFS
                     VCNTGNILSRDIIENSGDVPYLCASRDNNSVSSYIAYDDSLLEKGNCIFIGGKTFVVT
                     YQERDFFSNDSHNLRLYVNNEDGRTKFAYLGIISCIYRSISHKYSWGDSVSNRKIKND
                     VIWLPVKNKSPDFCYINDLISVVHKLVIKDVVQYAERKMNAYRQAITSRGYDDSGVS"
BASE COUNT          256 a          164 c          128 g          239 t
ORIGIN      
        1 cacctttcta aggggggcag ggtaacccac agaattccgg atacaaaaaa cccgcgcatc
       61 ggcgggttaa gcagcgtggc aatgtaacca ttcttatcat gatatgagga tttttacgat
      121 tgtaaaatgt tttttaactg acaccagaat catcataacc gcgacttgtt atagcttgcc
      181 tgtatgcgtt cattttgcgt tctgcgtact ggacaacatc tttaatgaca agcttatgaa
      241 caactgaaat aaggtcattt atataacaaa agtcaggtga cttatttttc actggcaacc
      301 aaataacatc gttcttaatt tttctattac taacactatc cccccatgaa tatttatgtg
      361 aaatagacct ataaatacat gaaataatgc ccaaataagc aaactttgtc cttccatcct
      421 cattgttaac atagagtcgc aaattatgac tgtcgtttga aaaaaagtct cgctcctgat
      481 aagttacaac gaatgtctta ccaccaataa aaatacagtt ccctttttcc aagagtgagt
      541 catcataggc gatataagaa ctaacagagt tattatcacg gctagcacaa agataaggaa
      601 catccccact attttctatg atgtctctag acaatatgtt tcctgtattg cacactgaga
      661 ataactcagt aacaggaaag cctgaaaatt caatattttt aaaatgttcc aggataagat
      721 aatctttttc cgttagttgg taatcactca gcccagcagc tatcaggtat gcttccagct
      781 cttctat
//
LOCUS       DAZGAN020000387          774 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZGAN020000387 DAZGAN020000000
VERSION     DAZGAN020000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.394"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            2..>774
                     /locus_tag="ACHKYO_RS27020"
                     /old_locus_tag="pgaptmp_005404"
     CDS             2..>774
                     /locus_tag="ACHKYO_RS27020"
                     /old_locus_tag="pgaptmp_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432026.1"
                     /translation="MSSSLVVGLLSVSSFTRANEYSYSALDVLGKHGSTYTIVYNSDS
                     DTAYVKWKKVPSGVEGIKDGDIVQGLSKINKDGTVEVTTKDGTSTYRVYDREFHNLMN
                     SLVKQDKENTLGKKEGTQTDLVSDADIRNLFGRIAAISEMETNVGLDEYGRVATSAES
                     IKERVSLQTYLATEAANSAIGLDKNGKLTLNETDIVTPLRINDGVVTIAKDVEEQKEG
                     LAIQKKGLSELSSRTDIIDAATAANTNKIDSRTTVGVNA"
BASE COUNT          245 a          129 c          194 g          206 t
ORIGIN      
        1 tctgtcatct tctctggttg taggtctgct gagtgttagt tcttttacac gtgcaaatga
       61 atatagctac agcgccttgg atgtgttagg taaacacgga agtacttaca ccattgttta
      121 taatagtgat tctgataccg cttatgttaa atggaaaaaa gtacctagcg gtgttgaggg
      181 gattaaagat ggagatattg tacaaggatt atcaaaaatt aataaagatg ggactgtaga
      241 agttactacc aaggatggca ctagtacata tcgtgtgtat gaccgggagt ttcataacct
      301 aatgaattca ctggtcaagc aggataaaga aaacactctt ggaaagaaag agggtactca
      361 aacagacctt gtatctgatg ctgatattcg gaatcttttt ggcagaattg ctgcgatctc
      421 tgaaatggaa actaatgttg gtctggatga atatgggcga gtggcaacta gtgcagagag
      481 tattaaagaa cgcgtgtctt tgcagactta tctggctaca gaggctgcaa atagcgcaat
      541 tggcctagat aaaaacggta agctaactct gaatgagaca gatattgtaa cgccgttacg
      601 tatcaatgac ggcgttgtca ctatagcgaa agatgtggaa gaacagaaag aagggctggc
      661 gatccagaag aaaggactgt ctgagctttc atcccgcaca gatatcattg atgcagcaac
      721 ggctgcgaat acgaataaaa ttgattcccg aaccaccgtt ggtgtgaatg cgga
//
LOCUS       DAZGAN020000388          765 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZGAN020000388 DAZGAN020000000
VERSION     DAZGAN020000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.395"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>765
                     /locus_tag="ACHKYO_RS27025"
                     /old_locus_tag="pgaptmp_005405"
     CDS             <1..>765
                     /locus_tag="ACHKYO_RS27025"
                     /old_locus_tag="pgaptmp_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4432027.1"
                     /translation="VPWICGDTTYLWKQKYTSQYPVVYGAYQNKEAQKIYFVPLMTDE
                     KGTNTPTNLPAEDPDLAEAGYYGAASRSNGNWTSSDRATHFSSWARRGIVSDRLATAI
                     LMHAGKTAAFLKGTPAPFSVVVAPPAMAGSDSATTESPQPSTSAPQPGAGTQPSPEVP
                     PATETPARTTAVAYRADTQEGKLKEQGWTGADGKASVVDDSAAESGKAMRVEKVQGQQ
                     TAWKIAHDVPASKAAELLQRGGEIAFRFKIPEGVQL"
BASE COUNT          204 a          214 c          201 g          146 t
ORIGIN      
        1 ttgttccctg gatttgcggc gataccacct atctctggaa acaaaaatat acctctcagt
       61 acccggtggt ttacggtgca tatcagaaca aagaagcgca aaaaatttat tttgtgcccc
      121 tgatgaccga tgagaaaggt actaacacgc caacaaacct gcctgccgaa gatcctgatc
      181 tggcagaagc cggttattac ggtgctgcat cccgcagtaa tggaaactgg acctcttcag
      241 atcgcgccac gcatttcagt tcatgggccc gtcgcggcat tgtttctgac cgtctggcaa
      301 cggcgatcct gatgcatgca gggaagacag ccgccttcct gaaaggcaca cccgcaccgt
      361 tcagcgtggt cgtagcgcct cccgccatgg caggttcaga cagcgccacc acggaaagcc
      421 cgcagccctc cacatctgca cctcagccgg gcgcaggcac acagccatca ccggaagttc
      481 caccggcaac tgaaacaccg gcaagaacaa ctgccgtcgc ttatcgtgca gatacacagg
      541 aagggaaact gaaagaacag ggctggacgg gggcagacgg aaaagcctct gtcgtagatg
      601 attctgctgc tgagagcggt aaagccatgc gagtggaaaa agtccagggg caacaaacag
      661 cctggaaaat tgcccacgat gtgcctgcaa gtaaagcagc tgaattactt cagagaggcg
      721 gtgaaattgc cttccgtttc aaaattcccg aaggtgttca gctgg
//
LOCUS       DAZGAN020000389          763 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZGAN020000389 DAZGAN020000000
VERSION     DAZGAN020000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.396"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            42..161
                     /locus_tag="ACHKYO_RS27030"
                     /old_locus_tag="pgaptmp_005406"
                     /pseudo
     CDS             42..161
                     /locus_tag="ACHKYO_RS27030"
                     /old_locus_tag="pgaptmp_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            121..763
                     /locus_tag="ACHKYO_RS27035"
                     /old_locus_tag="pgaptmp_005407"
                     /pseudo
     CDS             121..763
                     /locus_tag="ACHKYO_RS27035"
                     /old_locus_tag="pgaptmp_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT          261 a          110 c          167 g          225 t
ORIGIN      
        1 accttatcaa tgatgaggga attaaatagt tttcataatg agttacagca caacgttggg
       61 tattgactga gcaagatgaa cgacaaggac aacgacatca gccttatgag gagcaagaaa
      121 atgaaagcaa attcgaaaac gatcagaagg atgaaagtta atgttcggtc tgaggaaagt
      181 aaatcgaaac actcatacgt ttgaaaccac ttttcaaaat tggaaagaaa atggcgaaga
      241 tgttgcatta ttaatgcccg aatttagtgc taaatggtta ccgatagcag aagagagtgg
      301 cagctggtct ggatgggtat tgcttggaga aatatttccc ttaatttcat ctgagctcgc
      361 tgggatggct ttaatgccag aaacggaaag gttaataggg gaatggctca gtttatccag
      421 ttccccgtta aatctaaagc atcctgaact aaaatataat cgtttgcacg taggtaaagt
      481 atttgatgga gtattgagcc ctgctcaacc actaataaga atatgaacag gggaattgaa
      541 tctttggtta gataaagtca cagtctgcca atacggaaac gctccagtgt tagacaaaaa
      601 atcattatat tggtccattc attttgtaat tggatttagt aaaacatgtt acagaagtct
      661 tgttgatatt gaagttggtg atgttttatt aatctcaaat aatttggctt atgcggttat
      721 ttataataca aaaatttgtg atttaattta tccagaggag tta
//
LOCUS       DAZGAN020000390          758 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZGAN020000390 DAZGAN020000000
VERSION     DAZGAN020000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.397"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            166..447
                     /locus_tag="ACHKYO_RS27040"
                     /old_locus_tag="pgaptmp_005408"
     CDS             166..447
                     /locus_tag="ACHKYO_RS27040"
                     /old_locus_tag="pgaptmp_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432028.1"
                     /translation="MTEAEILGLIRRVAGISQQADEQATQPDSMTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
BASE COUNT          219 a          157 c          186 g          196 t
ORIGIN      
        1 gtgattttat cgtcatcatc atcatgtgtg taacctgttg tttttaatgt ggttgattca
       61 aaaaagatga tgattgtggt taatttttgt tcgacatctt ttagcgtgac agattcttta
      121 caaaaaatct gagcttgttt tcttcaccag cgcgatgggg gcacaatgac agaagccgaa
      181 atactgggat taatccgccg cgtcgccgga atcagccagc aggctgacga acaggccacg
      241 cagccggaca gcatgaccgc cgaaaattat gcgcgtgttg ttgctgaggt gatgcgccgt
      301 gatggtatcc agcttaatga tgcggatatg cgcgacatac ggatccgcgt tctggaaatg
      361 ctggcctaca atcgccgcgt tgaactgtat cgggagaaag aaaaaattac gtaccactgg
      421 aaaaagccgg agcggttacg gcgttaacga gttgtgacat accgtgacgg agagtgacaa
      481 ccagtgacac gacgtgacaa aaagacggta tttaaacagg gggagtattt aatacccctc
      541 ccaccagcct gattaactgt taaccggaaa aaaaatccgg tagggttctc gatatgggga
      601 tccccatatc ggggaacatc caccagcttc ataacggata gccggaaaat gataacggat
      661 aactacgaaa ttttcgcagt ttaataaacc tctgggaaaa ctcgccttta aattgtacac
      721 tttttcagca aatgatttta gttatacgtt tggttata
//
LOCUS       DAZGAN020000391          752 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZGAN020000391 DAZGAN020000000
VERSION     DAZGAN020000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.398"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            3..329
                     /locus_tag="ACHKYO_RS27045"
                     /old_locus_tag="pgaptmp_005409"
     CDS             3..329
                     /locus_tag="ACHKYO_RS27045"
                     /old_locus_tag="pgaptmp_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432029.1"
                     /translation="MGGSASEVDSFLNKLNITDYKYYDFSQIYSCSDRTLNDYAVIFI
                     RDALNASEHIIIFNCTGWPDLNNESAVMQFARVARKSGKQLIVAVREQDMKKMEAEFG
                     RIIKIH"
     gene            609..>752
                     /locus_tag="ACHKYO_RS27050"
                     /old_locus_tag="pgaptmp_005410"
     CDS             609..>752
                     /locus_tag="ACHKYO_RS27050"
                     /old_locus_tag="pgaptmp_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012368889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4432030.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEAR"
BASE COUNT          211 a          140 c          196 g          205 t
ORIGIN      
        1 ttgtgggggg ctcagcttct gaagttgata gctttttaaa taaactgaat attactgatt
       61 ataagtatta tgatttcagc cagatttaca gttgttctga cagaacatta aatgattatg
      121 cggttatttt tatcagggat gcactgaacg ccagtgagca tattattatt tttaattgta
      181 caggatggcc tgatttgaat aatgaatctg ccgtcatgca gtttgccaga gtggcacgaa
      241 aatcaggaaa acaactgata gtcgcagtaa gagaacagga tatgaaaaaa atggaagcag
      301 agtttggaag gatcataaaa atccactgac gccttcgcgt tgctcagttg tccaaccccg
      361 gggacgggaa aaagcaagtt ttccccgccc ccggcgtttc tgtagtgaaa agcgggaaat
      421 ggagggaatg gtcagtaaca gggaatgtgg tattatcgcg gcgggtgtct gagcctttct
      481 ggttcaggca agacgcaggt accagaaatg cgaagacccc acttgttaat ccattaactc
      541 gtgaggcctg catgaagtac ttgaacacta ctgattgtag cctcttcctt gcagagaggt
      601 caaagtttat gacgaaatat gcccttatcg ggttgctcgc cgtgtgcgcc acggtgttgt
      661 gtttttcact gatattcagg gaacggttat gtgagctgaa tattcacagg ggaaatacag
      721 tggtgcaggt aactctggcc tacgaagcac gg
//
LOCUS       DAZGAN020000392          751 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZGAN020000392 DAZGAN020000000
VERSION     DAZGAN020000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.399"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            53..>751
                     /locus_tag="ACHKYO_RS27055"
                     /old_locus_tag="pgaptmp_005411"
     CDS             53..>751
                     /locus_tag="ACHKYO_RS27055"
                     /old_locus_tag="pgaptmp_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4432031.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYH"
BASE COUNT          248 a          130 c          165 g          208 t
ORIGIN      
        1 catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg
       61 taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta
      121 tgcaaaattc ggtgtagaaa tctatgctgc tgctaaacaa ggtgaacccg atccagaatt
      181 aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt
      241 tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggacg
      301 ttatgaaggc tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt
      361 taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc
      421 ggcaggttct gtcagctata tgtttgacaa tacgggtgtg attgtattta aaggatcaga
      481 ccctgaccat atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga
      541 agaagaaggt aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc
      601 tctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc
      661 tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga
      721 agatgacgac gatgtacaaa aagtttatca t
//
LOCUS       DAZGAN020000393          749 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZGAN020000393 DAZGAN020000000
VERSION     DAZGAN020000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.400"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>749
                     /locus_tag="ACHKYO_RS27060"
                     /old_locus_tag="pgaptmp_005412"
     CDS             <1..>749
                     /locus_tag="ACHKYO_RS27060"
                     /old_locus_tag="pgaptmp_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HGU4432032.1"
                     /translation="LNLKMMNQPYRADILNAITKVVDSGWYIQGEEVRNFEHEFASFI
                     GSKYCVGVANGLDALSITLKAWILLGKIKEGDEVIVPANTFIATVLAITQNHLKPIFV
                     EPDEETFNINVDMIRNVITEKTKVIIPVHLYGQVAEMNKIRELADLHDLLILEDSAQA
                     HGAMYNNLKAGNLGDAGAFSFYPGKNLGALGDAGAVVTDNQELAETIRAIGNYGAHVK
                     YKHDVQGVNSRLDEIQAAILRVKLKYINNEI"
BASE COUNT          259 a          109 c          165 g          216 t
ORIGIN      
        1 tcctaaatct aaaaatgatg aatcaacctt atcgagcgga tatactaaat gcaataacta
       61 aagttgttga ttcggggtgg tatattcaag gtgaagaagt cagaaatttc gaacatgagt
      121 ttgcatcatt tattggttcg aagtattgtg tcggtgttgc caatggctta gatgcattga
      181 gcattacctt aaaagcatgg atattattag gcaaaattaa agaaggggat gaggtaatag
      241 ttccagcaaa tacatttatt gcaactgtat tagctatcac ccaaaatcat cttaaaccaa
      301 tatttgtaga accagatgaa gaaacattca acattaatgt tgatatgatt agaaatgtca
      361 tcacggagaa gactaaagtt attatacctg ttcatcttta tgggcaggtg gcagaaatga
      421 ataaaattcg tgaattagct gaccttcatg atctgttgat tttggaggac tctgcgcaag
      481 cacatggagc catgtataat aaccttaagg caggaaacct tggtgatgca ggcgctttta
      541 gtttctaccc aggtaaaaac ttgggggcat taggggatgc tggtgctgta gtgactgaca
      601 atcaagagct ggcagaaact ataagagcaa ttggtaatta tggtgcacat gtaaaatata
      661 aacatgatgt acaaggggtt aatagccgtc tagatgagat tcaagcagcg attctacggg
      721 ttaaattaaa atatataaac aatgaaata
//
LOCUS       DAZGAN020000394          738 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZGAN020000394 DAZGAN020000000
VERSION     DAZGAN020000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.401"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>738
                     /locus_tag="ACHKYO_RS27065"
                     /old_locus_tag="pgaptmp_005413"
     CDS             <1..>738
                     /locus_tag="ACHKYO_RS27065"
                     /old_locus_tag="pgaptmp_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4432033.1"
                     /translation="VPWICGDTTYIWKQQYTSQYPVVYGAYQNKEAQKIYFVPLMTDE
                     KGANTPTNLPAEDPDLAEAGYYGAASRSNGNWTSSDRATHFSSWARRGIISDRLATAI
                     LMHAGKTVSFLKGAPAPFSAVAAPPTTAGSDSTTTESPQPSTPAPQPGAGTQSSPART
                     TAVAYRADAQEGKLKEQGWTGADGKASVVNDSAAESGKAMRVEKVQGQQTAWKISHDV
                     PASKAAELLQRGGEIAFRFKIPEGVQL"
BASE COUNT          194 a          213 c          192 g          139 t
ORIGIN      
        1 ttgttccctg gatttgcggc gataccacct atatctggaa acaacaatat acctctcagt
       61 acccggtggt ttacggtgca tatcagaaca aagaagcgca aaaaatttat tttgtgcccc
      121 tgatgaccga tgagaaaggt gctaacacgc caacaaacct gcctgccgaa gatcctgacc
      181 tggcagaagc cggttattac ggtgctgcat cccgtagtaa cggaaactgg acctcttcag
      241 atcgcgccac gcatttcagt tcatgggccc gccgcggcat tatttctgac cgcctggcaa
      301 cggcgatcct gatgcatgcc gggaagaccg tctccttcct gaaaggtgca cccgcaccgt
      361 tcagcgcggt cgcagcacct cccaccacgg ccggttcaga cagcaccacc acggaaagcc
      421 cgcagccctc cacacctgca cctcagccgg gtgcaggtac gcagtcatca ccggcaagaa
      481 caacggccgt cgcttatcgc gcagacgcac aggaagggaa actgaaagaa cagggctgga
      541 caggggcaga tggaaaagcc tccgtcgtaa atgactctgc tgctgagagc ggtaaagcca
      601 tgcgagtgga aaaagtccag gggcaacaaa cagcctggaa aatttcccac gatgtgcctg
      661 caagtaaagc agctgaatta cttcagagag gcggtgaaat tgccttccgt ttcaaaattc
      721 ccgaaggtgt tcagctgg
//
LOCUS       DAZGAN020000395          735 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZGAN020000395 DAZGAN020000000
VERSION     DAZGAN020000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.402"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..312
                     /locus_tag="ACHKYO_RS27070"
                     /old_locus_tag="pgaptmp_005414"
     CDS             <1..312
                     /locus_tag="ACHKYO_RS27070"
                     /old_locus_tag="pgaptmp_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HGU4432034.1"
                     /translation="PRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAY
                     VNPLVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGG
                     V"
     gene            309..>735
                     /locus_tag="ACHKYO_RS27075"
                     /old_locus_tag="pgaptmp_005415"
     CDS             309..>735
                     /locus_tag="ACHKYO_RS27075"
                     /old_locus_tag="pgaptmp_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HGU4432035.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAA"
BASE COUNT          178 a          151 c          234 g          172 t
ORIGIN      
        1 ccgcgcgtat tctggctgtt tcctgtccct gacaaagaga taagtattga tgcagtggta
       61 agccggatac cggaggcagt gtatgttctg acgcaggacg atgatacgcc agttccactg
      121 gaagaggctt atgttaaccc actggtggac tggatgttgt ttcgtgcttt cagtaaggat
      181 gctgccggtg gtgcagaatc ggggctggct gcgcagcatt atcagagttt tgttgagcaa
      241 cttgggatca aacagggggc agacagtgca ttgtctgccc gtaaaaaagt gtttaacgga
      301 ggtggagtgt gagtgttgtt gtttcgggga cgctgaaatc tcctgatggt gaggcgatat
      361 caggagcaaa tattaccctg acggcgctga cagtttcacc ggatgcgctc agcggcacca
      421 gtgcgtcggc agtgacccgt gaaggtggat attacggaat gacgatggat ccgggggagt
      481 atgcggtttc ggtgacggtg aaagggaaga ctgctgtcta cggacgtgtg cgtattgagg
      541 ggaccgaaag tacggtgacg ctcaatatgc tgttacgccg cagtcttgtt gaggtgagca
      601 tacccggaga gctgctgaca gatttccggc agatacagaa taatgtggct gatgaccttg
      661 ctactattcg tcgcctgaat gaagacacgg cgacaaaaaa cactcaggcc acacagtcaa
      721 aagaaagtgc agcag
//
LOCUS       DAZGAN020000396          726 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZGAN020000396 DAZGAN020000000
VERSION     DAZGAN020000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.403"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            35..>726
                     /locus_tag="ACHKYO_RS27080"
                     /old_locus_tag="pgaptmp_005416"
     CDS             join(35..284,284..>726)
                     /locus_tag="ACHKYO_RS27080"
                     /old_locus_tag="pgaptmp_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HGU4432036.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHY"
BASE COUNT          166 a          186 c          205 g          169 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 cgcccgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAZGAN020000397          719 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZGAN020000397 DAZGAN020000000
VERSION     DAZGAN020000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.404"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..278
                     /locus_tag="ACHKYO_RS27085"
                     /old_locus_tag="pgaptmp_005417"
     CDS             <1..278
                     /locus_tag="ACHKYO_RS27085"
                     /old_locus_tag="pgaptmp_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HGU4432037.1"
                     /translation="ATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDEKQVPTGAFFA
                     GAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            275..601
                     /locus_tag="ACHKYO_RS27090"
                     /old_locus_tag="pgaptmp_005418"
     CDS             275..601
                     /locus_tag="ACHKYO_RS27090"
                     /old_locus_tag="pgaptmp_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HGU4432038.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTVVKDDDSEAVGIIRSLLAGEENARLRAAHWLH
                     ERGLKV"
     gene            598..>719
                     /locus_tag="ACHKYO_RS27095"
                     /old_locus_tag="pgaptmp_005419"
     CDS             598..>719
                     /locus_tag="ACHKYO_RS27095"
                     /old_locus_tag="pgaptmp_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease"
                     /protein_id="HGU4432039.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAA"
BASE COUNT          165 a          171 c          233 g          150 t
ORIGIN      
        1 ctgccaccag cgaagtgtgg tggccggaag atgcagatca tattgctttt attcgcgggc
       61 gtattggttt tgaactgcct gcctggttta tcccgaaaga tgagaagcag gtgccgacag
      121 gcgctttctt cgctggtgct attgctgttt tcgacaagac ctggaaggga ccggcaatca
      181 gctacatcgg gcgtgatgaa cttgaggcat gtggtgaggc gtttctggcg caggttcgcc
      241 agcaggcgga aaaactggtc agggagatgg cggcatgacg acgttaactc aatgccagca
      301 gcaggtgctg gatatgctga tttcttatca gaaagaacgt ggcttcccgc caaccaatca
      361 ggaggtggca accatgctgg gataccgttc agtgaatgca gcggtggagc atcttcgcgc
      421 actggagaaa aaaggcgtca tcacgataaa gcgtggtgtg gcccggggga tcacgcttca
      481 taccgtggtg aaggacgacg acagcgaggc ggtcgggatt atccgctcac tgcttgccgg
      541 tgaggaaaac gccaggctgc gtgcagccca ttggttacat gagagaggcc tgaaagtatg
      601 aagctgatcc tgccttttcc gcccagcgtg aacacgtact ggcgacaccc caacaaaggg
      661 gcgtttgctg gtaagagcct gataagcgcg gcggggcgaa aattccagag cgcggcgtg
//
LOCUS       DAZGAN020000398          712 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZGAN020000398 DAZGAN020000000
VERSION     DAZGAN020000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.405"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..142
                     /locus_tag="ACHKYO_RS27100"
                     /old_locus_tag="pgaptmp_005420"
     CDS             <1..142
                     /locus_tag="ACHKYO_RS27100"
                     /old_locus_tag="pgaptmp_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4432040.1"
                     /translation="VCVLYLLSGGYRAVRDFWRRQIDKRAAEKISASQSAGSKPEEPI
                     TP"
     gene            81..452
                     /locus_tag="ACHKYO_RS27105"
                     /old_locus_tag="pgaptmp_005421"
     CDS             81..452
                     /locus_tag="ACHKYO_RS27105"
                     /old_locus_tag="pgaptmp_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4432041.1"
                     /translation="MRKSAPVSQPEANPKSPLLLNNPFQRENPMSEVKPLVTADAVKD
                     ALRSEDVRGALKQKVRQTFEAQIEADVDAILDTILGEQPGEQPEPEPQPEDITSENGD
                     IQPESPVVDATEAQPEPGTML"
     gene            528..>712
                     /locus_tag="ACHKYO_RS27110"
                     /old_locus_tag="pgaptmp_005422"
     CDS             528..>712
                     /locus_tag="ACHKYO_RS27110"
                     /old_locus_tag="pgaptmp_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4432042.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIK"
BASE COUNT          188 a          168 c          211 g          145 t
ORIGIN      
        1 ggtctgtgtg ctgtatcttc tttcgggtgg gtacagggca gtgcgcgatt tctggcgcag
       61 gcagattgat aaaagggccg ctgagaaaat cagcgccagt cagtcagccg gaagcaaacc
      121 cgaagagccc attactcctt aacaacccct ttcaacgaga aaaccctatg tcagaagtaa
      181 aacccctggt cactgctgat gcagtgaaag acgcgctgcg ctcagaagat gtgcgtggag
      241 cactgaaaca gaaagtccgc cagacatttg aagcgcagat agaagcagat gtggatgcga
      301 ttctggatac gatactggga gagcagccag gcgaacagcc agagccggaa ccgcaaccgg
      361 aagatatcac ctcagaaaac ggcgatattc aaccggaatc accggtggtt gatgcgactg
      421 aagcacaacc cgaaccgggc accatgctgt aagggtgagt cagggccatc agtaaagtac
      481 tgctggcctt tttcatgttg tgagcttccg tgtgcgggag acggggtatg taccagatgg
      541 aaaaaatcac aacaggtgtg tcatacacca cgtcagcggt gggaacgggc tactggttcc
      601 tgcagttgct ggacagggtt tccccgtctc agtgggcggc aataggcgtg ctggggagtc
      661 tgctgtttgg gctgctgaca tatctgacga acctgtattt caagattaaa ga
//
LOCUS       DAZGAN020000399          708 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZGAN020000399 DAZGAN020000000
VERSION     DAZGAN020000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.406"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>708
                     /locus_tag="ACHKYO_RS27115"
                     /old_locus_tag="pgaptmp_005423"
     CDS             <1..>708
                     /locus_tag="ACHKYO_RS27115"
                     /old_locus_tag="pgaptmp_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HGU4432043.1"
                     /translation="LTAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAI
                     CTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLT
                     GKLIRSEDEGLVTHWHYDEADRLTHRTVNGETAEQWQYDERGWLTDISHISKGHRVTV
                     HYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYG
                     SGYLAGMKLGDTPLVEYT"
BASE COUNT          177 a          210 c          220 g          101 t
ORIGIN      
        1 gttaactgcg gtgaaagaca cgcagggcca tgaaacgcgg tatgaataca acgccgccgg
       61 tgacctgacc gccgtcattg ccccggacgg cagcagaaac gggacacagt acgatgcgtg
      121 gggaaaagcc atctgtacca cgcagggcgg tctgacgcgc agtatggaat acgatgctgc
      181 cggacgggtc atccgcctga ccagtgaaaa cggcagccac accaccttcc gttacgatgt
      241 actcgaccgg ctgatacagg aaaccggctt tgacggccgc acacagcgtt atcaccacga
      301 cctgaccggc aaacttatcc gcagcgagga tgaggggctg gtcacccact ggcactatga
      361 cgaagcagac cgcctcacgc accgcaccgt gaatggcgaa accgcagagc agtggcagta
      421 tgacgaacgc ggctggctga cagacatcag ccatatcagc aaagggcacc gggtgacggt
      481 gcattacggg tatgatgaga aaggccggct gaccggtgag cgtcagacgg tgcatcaccc
      541 gcagacggaa gcactgctct ggcagcatga gaccagacat gcgtacaacg cgcaggggct
      601 ggcgaaccgc tgtataccgg acagcctgcc cgccgtggaa tggctgacct acggcagcgg
      661 ttacctggca ggcatgaaac tcggcgacac accgctggtg gagtacac
//
LOCUS       DAZGAN020000400          707 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZGAN020000400 DAZGAN020000000
VERSION     DAZGAN020000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.407"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..388)
                     /locus_tag="ACHKYO_RS27120"
                     /old_locus_tag="pgaptmp_005424"
     CDS             complement(<1..388)
                     /locus_tag="ACHKYO_RS27120"
                     /old_locus_tag="pgaptmp_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4432044.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCAERAN"
BASE COUNT          227 a          130 c          138 g          212 t
ORIGIN      
        1 cattcgcacg ttcagcacaa tcagttgtca tgcactcgga ataaatcata ccgtgaatag
       61 tgctgttttt gattgtcaga ctattggcgt tggtattggt agaaataccg tcgtccagat
      121 agtaggtaga aatcacgccg ttaacagtag cgttattaat gaccgggtag atatcgccgt
      181 tgtaataact atcagcggca tagttgttcc agcctacata accatcatag taaacgtcat
      241 cagcataggt ggcgtcgttg tagtgatgga aagtattgta agtttgacca gaaatatcgt
      301 tggtcgctgc attagcctga gaggtgatag ccagagtgca agccaatgct aattgtgata
      361 ctacaagttt ctttttccag gagtgcattt gtcatccctc ctcagggacg taataagttg
      421 atccatcaat tatcaatgca tagaaaacat attatgtttc gcgctaacga ttatgctgcg
      481 ttacagacga atggttcaat tattctttcc cctaagtccg aatgtggaca tatataattt
      541 atggagaaaa acacgcatca aattgataaa aggacgatat ttcaacatgt tgcagataat
      601 gttatttttg atacatacgc gttataaata tcaaaaacac aaaccagata aatatcttat
      661 caatatacaa caaagtttga attattcaat ttatagcagc acaacca
//
LOCUS       DAZGAN020000401          701 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZGAN020000401 DAZGAN020000000
VERSION     DAZGAN020000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.408"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>701
                     /locus_tag="ACHKYO_RS27125"
                     /old_locus_tag="pgaptmp_005425"
     CDS             <1..>701
                     /locus_tag="ACHKYO_RS27125"
                     /old_locus_tag="pgaptmp_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4432045.1"
                     /translation="VDNGARFEPLSGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDT
                     PRVVKGVRFSLRLTSGSGENSRLVTSAITADTEHRFSGLPLGEYTLTVRAINSYGQQG
                     EPATTAFRINAPAAPASIELTPGYYQITAVPVLAVYDPTVQSEFWFSEKRITDMAQVE
                     TFARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLDFF
                     RGEIGKTHLAQGMWEL"
BASE COUNT          149 a          187 c          233 g          132 t
ORIGIN      
        1 gtggataacg gggcccgctt tgagccgctc tccggttcac tgaacagcgt catcccgccg
       61 gcagtgcagc acctcacggt ggaggtgagt gcctcagacg gccagtatct ggcgctggca
      121 aaatgggaca cgccgcgggt ggtgaagggc gtgcgcttca gtctgcgcct gaccagtggc
      181 agtggtgaaa acagccgcct ggtgaccagc gccatcactg ccgacacgga gcaccgtttc
      241 agtggcctgc ctctggggga atacaccctg acggtcaggg cgataaacag ctacggccag
      301 cagggcgaac ctgccaccac cgcgttccgg attaatgcgc ctgcagcacc ggccagcatt
      361 gagctgacgc cggggtatta tcagataaca gcagtaccgg tgctggcggt gtatgacccg
      421 acggtacagt ctgaattctg gttctcagaa aaacgcatca cggacatggc acaggtggaa
      481 acctttgccc gttatctggg gacaggcagc cagtggagtg tctccggccc gcacattaag
      541 ccggggaagg atttctggtt ttatgtgcgc agcgtcaacc tggtggggaa atctgcgttt
      601 gtggaagcca gtggccgggc gagcaatgat gcggaagggt atctggactt tttcagagga
      661 gaaatcggga agacacatct ggcacagggg atgtgggagc t
//
LOCUS       DAZGAN020000402          697 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZGAN020000402 DAZGAN020000000
VERSION     DAZGAN020000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.409"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..297
                     /locus_tag="ACHKYO_RS27130"
                     /old_locus_tag="pgaptmp_005426"
     CDS             <1..297
                     /locus_tag="ACHKYO_RS27130"
                     /old_locus_tag="pgaptmp_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4432046.1"
                     /translation="NKREAFPVSAEYYKQQQRLKGMRKRAEEKIRDAEDEVVNVLEHK
                     PQEPWLENIYRPVGNTVAVQQPAVDDEPDEEYERNFQRGLQLLEAKLKENDPLA"
     gene            369..>697
                     /locus_tag="ACHKYO_RS27135"
                     /old_locus_tag="pgaptmp_005427"
     CDS             369..>697
                     /locus_tag="ACHKYO_RS27135"
                     /old_locus_tag="pgaptmp_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4432047.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTAT"
BASE COUNT          203 a          155 c          198 g          141 t
ORIGIN      
        1 aataaacgcg aagccttccc ggtcagcgcg gaatactaca aacagcagca acgccttaaa
       61 ggaatgcgta aacgcgcaga ggaaaaaatc cgtgatgccg aggatgaggt ggtcaacgtg
      121 ctggagcaca agccgcagga gccctggctg gaaaacatct atcgcccggt aggtaatacg
      181 gtggccgttc agcaacctgc cgttgatgat gagcctgatg aagaatacga gcgtaatttc
      241 cagcggggat tgcagttgct cgaagcgaaa ttaaaagaaa atgacccgct ggcctgaaat
      301 aaaaaaataa cccgagcggc gactcaggtt atttgattaa acaaggtatc aaatgagagg
      361 ttaataatat gactgatatt aacgatgtaa tcaagaccat tgatgaactt attgatggcg
      421 gcgtactgac gcagtatgcc atcgccagag aggcgggaat ttccgacggc acattatcgg
      481 ctttccgcaa ggggaaatat aaaggcgata acgccgctgt ggctgcttcc ctgcgttcct
      541 ggtatgaaaa ctggaataaa caaagcgcac tgccggaacc gccgcagttt gtggaaacgc
      601 agacagttca ggagctgcgc gcactgtttc aggcggttcg cctgatgggc tgtattaacg
      661 tcattgtggg cgtgccgggg gtaggtaaaa cggccac
//
LOCUS       DAZGAN020000403          695 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZGAN020000403 DAZGAN020000000
VERSION     DAZGAN020000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.410"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..624)
                     /locus_tag="ACHKYO_RS27140"
                     /old_locus_tag="pgaptmp_005428"
     CDS             complement(<1..624)
                     /locus_tag="ACHKYO_RS27140"
                     /old_locus_tag="pgaptmp_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU4432048.1"
                     /translation="MLKDGTGKAVPGCTIELKARRTTETVIVTTVAYGQPGETGSYSM
                     DVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKALAELEAV
                     AAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERGPKGERGE
                     TGPQGLQGVKGERGEKGEKGEPGGPDATTAQKGIVQLSSATDSDDETK"
BASE COUNT          102 a          264 c          144 g          185 t
ORIGIN      
        1 cttcgtttca tcatcactgt ccgttgcgct gcttaactgc acaattccct tctgtgccgt
       61 cgtcgcatcc ggtcctcccg gctcgccttt ttcgcccttc tctccccgtt cacctttcac
      121 accctgaagc ccctgtggtc ctgtctcacc acgctcaccc tttggccccc gctcgccggt
      181 atcgcctttt ggcccgggaa taccctgtgg cccggtgtct cccttatccc ccttcggtcc
      241 ccgcgcattc tctgcccgtt tcttcgcttc ctctgcactc gccgctgacg cttccgcacg
      301 tttcaggatt tctgcggcca ccgcttccag ctctgcaagg gctttcgggt aatactgtgc
      361 gtcctccagg tccatcagaa atttattcag cgttcccggt gcagagtccg ccttaaccag
      421 aatgtcaccc acatatgacg gcgcgtaccc ttccgtgttc agcgtcaccc ggtacaaccc
      481 cggctcaaca tccatactgt aactgccggt ttcccccggc tgaccatacg ccaccgtggt
      541 gacaatcacc gtctccgttg tgcggcgcgc tttcagctct atcgtgcatc ccggtaccgc
      601 ttttcccgta ccatccttca gcacacccga tattctgact gtcatggatt tcccccataa
      661 aaaaaccgca ggaccggttt cccggcctgc ggtaa
//
LOCUS       DAZGAN020000404          692 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZGAN020000404 DAZGAN020000000
VERSION     DAZGAN020000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.411"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>692
                     /locus_tag="ACHKYO_RS27145"
                     /old_locus_tag="pgaptmp_005429"
     CDS             <1..>692
                     /locus_tag="ACHKYO_RS27145"
                     /old_locus_tag="pgaptmp_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD5 domain-containing protein"
                     /protein_id="HGU4432049.1"
                     /translation="DTWQLLRTLPPSQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSL
                     RNKDNKGSNLFFSESRDELLTKAKVWFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNT
                     RSGDIFIAYGKNKMRVRGGFKSASDARKYIDSHRDELVRHVKEMREISREEQRNATNR
                     DRTGPERRKGNVSPEQFSDAFGFRGVQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVP
                     TKALSLNGRLGLA"
BASE COUNT          179 a          154 c          202 g          157 t
ORIGIN      
        1 gatacctggc aactgttacg cactctgcca ccctcacaga tggacagggc ttctgcttat
       61 cgggtggtat cgggtgtgta tcaggcggca ggagggaagc gttacgatcc gccacagaaa
      121 ctttattcac tgcgcaataa ggacaataag ggtagtaacc tctttttctc ggaaagcagg
      181 gatgaattac tgacaaaggc gaaagtctgg tttgcagagc aggaggaaaa atcacaggcg
      241 aaaggtgatg aaaaaacagc accgtcaccg gatgacaaaa tccgctttga cgtttaccgg
      301 aatacccgca gtggagatat ttttatcgct tacggtaaaa acaaaatgcg ggtgagaggt
      361 ggctttaagt cagccagtga tgcgcgtaag tacattgatt cacatcgtga tgagcttgtt
      421 cgtcatgtga aggagatgcg ggagatttcg cgtgaggagc agcgcaacgc caccaaccgc
      481 gaccgtaccg gaccagaacg ccgcaagggg aatgtttcac cggagcagtt cagtgatgcg
      541 tttggtttcc gtggtgtgca gtttggtaac tacgtggaag gtccgcgtcg tcaggctgat
      601 ttgaaccggg cttatgactc gctgcatgac cttgcggaag tactgaatgt accgacaaaa
      661 gcgctttccc tgaacggtcg tcttggcctg gc
//
LOCUS       DAZGAN020000405          689 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZGAN020000405 DAZGAN020000000
VERSION     DAZGAN020000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.412"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..506)
                     /locus_tag="ACHKYO_RS27150"
                     /old_locus_tag="pgaptmp_005430"
     CDS             complement(<1..506)
                     /locus_tag="ACHKYO_RS27150"
                     /old_locus_tag="pgaptmp_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HGU4432050.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSST"
BASE COUNT          207 a          160 c          131 g          191 t
ORIGIN      
        1 gtcgaagaac ctaatcttaa atagcctcca tcgacgtgac ccttctgttt aatattcagc
       61 gtccccgttc cggattgacc cacatttaaa ggccttgcat tatttccgct atcatacaat
      121 cgccaggtgc cacccaaaac attaacggtg ccctctgagt cctcattagc gccaatgacg
      181 ctggtagtaa tagtggttaa ggaggcatta ccgccagcca aaatattaag cactccagtg
      241 ccataccagc caaccaggta ggcatcataa gcaacatcag tatcaatatt ggtaatctga
      301 tcattatcaa cttccagact tgcaccagac gctgaaccgc ttagtgccag caaaacaccc
      361 agcgtcaatc tactgaattt cgttgtcagt ccagaggatt tacgcaaatt aaccgtcgat
      421 gttttacctg ccctgcgagt taattccgag caggcctgaa atacctgtag agtgcaattc
      481 catatcacgc gatagattct attcatataa acgctccata tacaaacaat acagccacga
      541 aagagatgag tcgtcagaca ttaaaaatga aacttattaa attgtcagag gtctgtattg
      601 agtgttagtt ggaggttgga aggtgtcaat ttggctaatt tgctaatgct ttcgaattga
      661 tagattaaaa attgcatagt tcttatttt
//
LOCUS       DAZGAN020000406          686 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZGAN020000406 DAZGAN020000000
VERSION     DAZGAN020000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.413"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..125)
                     /locus_tag="ACHKYO_RS27155"
                     /old_locus_tag="pgaptmp_005431"
     CDS             complement(<1..125)
                     /locus_tag="ACHKYO_RS27155"
                     /old_locus_tag="pgaptmp_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4432051.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNEL"
     gene            complement(125..466)
                     /locus_tag="ACHKYO_RS27160"
                     /old_locus_tag="pgaptmp_005432"
     CDS             complement(125..466)
                     /locus_tag="ACHKYO_RS27160"
                     /old_locus_tag="pgaptmp_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4432052.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVN"
     gene            complement(459..>686)
                     /locus_tag="ACHKYO_RS27165"
                     /old_locus_tag="pgaptmp_005433"
     CDS             complement(459..>686)
                     /locus_tag="ACHKYO_RS27165"
                     /old_locus_tag="pgaptmp_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4432053.1"
                     /translation="PLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGANGQIG
                     PEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          148 a          243 c          152 g          143 t
ORIGIN      
        1 agctcattgc agaaaaaata ccgctcaccg ccctgcaccg tcaggtcgat ttcccagagc
       61 accacccgcg gtgactgctc tgacttaacc gactcgttca ggctttcttc gtgaatatcc
      121 tgcatcagtt caccacctgc tctatcgtgc aactgaaatc actgtaccgg gcgttatccg
      181 taatactcca ctcccggcac accaccctga ccgtccggtt atgtttcggg ggcttccaca
      241 aaaaagcccg gtaaccacca tgccatgaca gaaacgcgga cagtgcctcc cgctcagcat
      301 ccgttgtcac ccgaaaaacc acctgaaagg tcttcagttg cgcattcagt cctgccggac
      361 gacgttgctg ataaccgtcg ccaaacttca ccgtcaccac cgacggtttc tccgtcacct
      421 gcatcccttc ccggggacac cagtgaaggg tcttaatctc atccactcag cattcctccg
      481 tcacgacgca tggataacat caccgcctgt acccgctggt caatcagttg cacaagggtg
      541 cccgccgctt ccggccctat ctgcccgtta gcgccgtcat tctgaatggc gatgtggtac
      601 accggggaat acaccagacc cgcactgccg ttcatactgc ccaccgcacg cacgccgagt
      661 gaaccatccg ctgcccgggt cagggg
//
LOCUS       DAZGAN020000407          686 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZGAN020000407 DAZGAN020000000
VERSION     DAZGAN020000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.414"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>686
                     /locus_tag="ACHKYO_RS27170"
                     /old_locus_tag="pgaptmp_005434"
     rRNA            <1..>686
                     /locus_tag="ACHKYO_RS27170"
                     /old_locus_tag="pgaptmp_005434"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          155 c          226 g          130 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaacc
//
LOCUS       DAZGAN020000408          684 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZGAN020000408 DAZGAN020000000
VERSION     DAZGAN020000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.415"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..488
                     /locus_tag="ACHKYO_RS27175"
                     /old_locus_tag="pgaptmp_005435"
     CDS             <1..488
                     /locus_tag="ACHKYO_RS27175"
                     /old_locus_tag="pgaptmp_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HGU4432054.1"
                     /translation="YPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKEY
                     GGLICKKQGEYLPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTK
                     ENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQGS
                     N"
     gene            490..>684
                     /locus_tag="ACHKYO_RS27180"
                     /old_locus_tag="pgaptmp_005436"
     CDS             490..>684
                     /locus_tag="ACHKYO_RS27180"
                     /old_locus_tag="pgaptmp_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HGU4432055.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKD"
BASE COUNT          267 a           79 c          133 g          205 t
ORIGIN      
        1 catatccgct gagcccggtg aatagcatgg atccattagg attatatgaa tttaaatcaa
       61 aaaatataga tgatattgga atatttgcat tggcaatgtg taatggagaa tcaattaacg
      121 agaataaaga atatggtgga ctaatatgta agaagcaagg tgaatattta cccatgaatc
      181 cgataagttc aaatgataat gatagtgtag acttgcgaaa tataaaatgc cctgaaggtt
      241 cagagagagt aggcgattat cacactcacg gtttttactc tgacgataaa ggaaataaag
      301 taacaaaaga aaatgatgtt tatgatagtc taaatttttc aagcaaagat ttaacgaatt
      361 cttatatgaa tggaatggga aaaaaagaat acagtagtta cttgggaaca ccaaataaca
      421 cctatctaaa atataatccc aaagctaaag ggaatggagt tacaattatc aggcaaggga
      481 gtaattaaaa tgaaggcaat acaatgttgt tatttccttg ctttagtctc cagtcattta
      541 atggctggag ttatgggtga tgataatata tttttaagta attcggattc agctgcttta
      601 tattgtgtgg ctaaaatatt tccagatgga gaaaatattt taatatataa agtaaatata
      661 attaatgcta gttcaaaaaa agat
//
LOCUS       DAZGAN020000409          673 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZGAN020000409 DAZGAN020000000
VERSION     DAZGAN020000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.416"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..673
                     /gene="espX1"
                     /locus_tag="ACHKYO_RS27185"
                     /old_locus_tag="pgaptmp_005437"
                     /pseudo
     CDS             1..673
                     /gene="espX1"
                     /locus_tag="ACHKYO_RS27185"
                     /old_locus_tag="pgaptmp_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
BASE COUNT          250 a          122 c           95 g          206 t
ORIGIN      
        1 tcttaatttt gtttgatgac tttttcagaa gccaggattt tcctaaacca tctccagtcc
       61 tccatcattt ttttcaatat actcatctga gagatgcaca tttttataga tgtaaactca
      121 tagaacatac agttcaattt tattttttaa acacaaaggc atcacattac ttagattgga
      181 tgtttttgat gacagaacaa gcgaatgtct ttctgaagag attaagatat atcaagaatg
      241 ctatgaaaaa ttcgttaagt tcttaaaggc aaacttcaac ccggaaatat acccacagct
      301 atatactcct gaaatatttt atgaggcctg cagaaatctc cagtcatttt atgctcatca
      361 ggagacgtca caaaacgcta aatatagtgc aatagacaag aaaaaaagtc atcttaacca
      421 ggaaatcaaa aagctaatta aaaaaaatat tttccctgag ctatataatg aacaatgcaa
      481 taaaatcccc tcctcatcga aagatgataa ccagaaaata acatggcaga attttaaaac
      541 tagcaattcg gcatattcaa aactatgtgg aaaattatct ttatttaaaa gcgtgcccag
      601 tcggttaata gaaaaatcag cttactgctc aactgaaaat atggccacta ataaatttga
      661 tgtcgtcttt tct
//
LOCUS       DAZGAN020000410          662 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZGAN020000410 DAZGAN020000000
VERSION     DAZGAN020000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.417"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..398
                     /locus_tag="ACHKYO_RS27190"
                     /old_locus_tag="pgaptmp_005438"
     CDS             <1..398
                     /locus_tag="ACHKYO_RS27190"
                     /old_locus_tag="pgaptmp_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030598 - rRNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-inactivating family protein"
                     /protein_id="HGU4432056.1"
                     /translation="EALRFRQIQREFRLALSETAPVYTMTPEEVDLTLNWGRISNVLP
                     EFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRSVNEEIQPECQITGDRPV
                     IRLNNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            411..>662
                     /gene="stxB2"
                     /locus_tag="ACHKYO_RS27195"
                     /old_locus_tag="pgaptmp_005439"
     CDS             411..>662
                     /gene="stxB2"
                     /locus_tag="ACHKYO_RS27195"
                     /old_locus_tag="pgaptmp_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit B"
                     /protein_id="HGU4432057.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEV"
BASE COUNT          200 a          124 c          171 g          167 t
ORIGIN      
        1 cagaagcctt acggttcagg caaatacaga gagaatttcg tctggcactg tctgaaactg
       61 ctcctgttta tacgatgaca ccggaagaag tggacctcac actgaactgg gggagaatca
      121 gcaatgtgct tccggagttt cggggagagg gtggtgtcag agtggggcga atatccttta
      181 ataatatatc agcgatactg ggcacagtgg cggttatact gaattgccat catcaggggg
      241 cacgttccgt tcgctccgtg aatgaagaga tacaaccaga atgtcagata actggcgaca
      301 ggccagttat aaggctaaac aatactttat gggaaagtaa taccgcagct gcttttctga
      361 atcgcagggc tcactcttta aatacatccg gagaataacg ggagttaaat atgaagaaga
      421 tatttgtagc ggctttattt gcttttgttt ctgttaatgc aatggcagct gattgtgcaa
      481 aaggtaaaat tgagttctct aagtataatg agaatgatac attcacagta aaagtggccg
      541 ggaaagagta ctggactaac cgctggaatc tgcaaccgct actgcaaagc gcacagttaa
      601 caggaatgac ggtaacaatc aaatcaaata cctgtgcgtc aggttcagga tttgctgaag
      661 tg
//
LOCUS       DAZGAN020000411          661 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZGAN020000411 DAZGAN020000000
VERSION     DAZGAN020000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.418"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            119..>661
                     /locus_tag="ACHKYO_RS27200"
                     /old_locus_tag="pgaptmp_005440"
     CDS             119..>661
                     /locus_tag="ACHKYO_RS27200"
                     /old_locus_tag="pgaptmp_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4432058.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSAGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGI"
BASE COUNT          142 a          180 c          194 g          145 t
ORIGIN      
        1 actgcgcggc ctttttcgta ttgcgggctg tagtcttcct cctgccattg tcctgtaact
       61 tccggacttc agcccgcccc tcatctgact cacaacatta tcccgaccgg gaggattcat
      121 ggcatttaaa cactatgacg tggtcagggc ggcgtcgccg tcagaccttg cggagcgact
      181 gactcaaaaa ctgaaggagg ggtggcagcc atttggcagt cctgtcgcca tcacgcctta
      241 taccctgatg caggccattg cggcggaagg tgatgtcacc acacctgtgt tggtgaagcc
      301 gtcggctgga gaaggcacag taatcagcgc caccagagac ccggagtatt actttgttgt
      361 ggttctggcg gggcagtcaa acagcatggc atatggtgaa ggccttccgt tgccggagac
      421 atatgaccgt ccggacccgc gtattaagca gctggcgcgc cgcagtacag tgacaccggg
      481 tggtgccgcc tgtaagtata acgacattat tccggcggac cattgtctgc atgatgtgca
      541 ggacatgagc cgtcttaacc atccgaaagc ggacctgtca aaggggcagt acggaaccgt
      601 ggggcagggg ctgcatatcg ccaaaaaact gctgccgttt ataccggcga atgcgggtat
      661 t
//
LOCUS       DAZGAN020000412          657 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZGAN020000412 DAZGAN020000000
VERSION     DAZGAN020000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.419"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..261
                     /locus_tag="ACHKYO_RS27205"
                     /old_locus_tag="pgaptmp_005441"
     CDS             <1..261
                     /locus_tag="ACHKYO_RS27205"
                     /old_locus_tag="pgaptmp_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HGU4432059.1"
                     /translation="EVYLKNNKLTVELLGRGFAWLDTGTHDSLIEAGSFVETVQKRQG
                     MMVACPEEIAWRNGWLNNSDLKSLATNMKNSYGSYLTKLILQ"
     gene            291..>657
                     /locus_tag="ACHKYO_RS27210"
                     /old_locus_tag="pgaptmp_005442"
     CDS             291..>657
                     /locus_tag="ACHKYO_RS27210"
                     /old_locus_tag="pgaptmp_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGU4432060.1"
                     /translation="MNDKSITPLISVITATFNSQRFILDTYKSLQAQSYKNWEWVVTD
                     DASEDKTVSILKELQSNDDRIKIFINADNKGAAWSRNNSMKESKGQYFAFIDSDDLWH
                     PEKLSRQIAFMGDNIDFSFT"
BASE COUNT          234 a           89 c          137 g          197 t
ORIGIN      
        1 gaagtttatc taaaaaacaa caagttgacg gtcgaactgc ttgggcgtgg tttcgcatgg
       61 ttagatacag gcacgcacga tagcctaatt gaggcgggaa gtttcgttga aactgtacaa
      121 aaaagacagg gtatgatggt agcgtgtcct gaagaaattg cttggcgaaa tggttggtta
      181 aataattctg atttgaaatc attagcaact aatatgaaaa atagttatgg ttcttattta
      241 acaaaactaa ttttacaata aataattatg caacgaataa cataatacgt atgaatgata
      301 aaagtattac accattaatt tcagttatta cggctacatt taattctcag cgttttattc
      361 tggatactta caaaagtctc caggcgcaga gctataaaaa ctgggaatgg gtcgttaccg
      421 atgatgcctc agaagataaa acggtaagta ttttaaaaga gttacagtca aatgatgaca
      481 ggataaaaat atttattaat gctgacaaca aaggagctgc ctggagtaga aataatagta
      541 tgaaggaaag taaaggtcaa tattttgctt tcattgatag tgatgatcta tggcatccag
      601 agaagttgtc taggcaaata gcattcatgg gtgataatat tgatttttct tttacca
//
LOCUS       DAZGAN020000413          655 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZGAN020000413 DAZGAN020000000
VERSION     DAZGAN020000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.420"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..554)
                     /locus_tag="ACHKYO_RS27215"
                     /old_locus_tag="pgaptmp_005443"
     CDS             complement(<1..554)
                     /locus_tag="ACHKYO_RS27215"
                     /old_locus_tag="pgaptmp_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU4432061.1"
                     /translation="MLKDGTGKAVPGCTIELKARRTTETVIVTTVAYGQPGETGSYSM
                     DVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKALAELEAV
                     AAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERGPKGERGE
                     TGPQGLQGVKGERGEKGEKGEPGGP"
BASE COUNT          100 a          242 c          139 g          174 t
ORIGIN      
        1 ggtcctcccg gctcgccttt ttcgcccttc tctccccgtt cacctttcac accctgaagc
       61 ccctgtggtc ctgtctcacc acgctcaccc tttggcccac gctcgccggt atcgcctttt
      121 ggcccgggaa taccctgcgg cccggtgtcc cccttatccc ccttcggtcc ccgcgcattc
      181 tctgcccgtt tttttgcctc ctccgcactg gccgccgacg cttccgcacg tttcaggatt
      241 tccgctgcca ccgcttccag ctctgcaagg gctttcgggt aatactgtgc gtcttccagg
      301 tccatcagaa atttattcag cgttcccggt gcagaatccg ccttcaccag aatgtcaccc
      361 acatatgacg gcgcgtaccc ttccgtgttc agcgtcaccc ggtacaaccc cggctcaaca
      421 tccatactgt aactgccggt ttcccccggc tgaccatacg ccaccgtggt gacaatcacc
      481 gtctccgttg tgcgtcgcgc tttcagctct atcgtgcatc ccggtaccgc ttttcccgta
      541 ccatccttca gcacacccga tattctgact gtcatggatt tcccccataa aaaaaccgca
      601 gtaccggttt cccggcctgc ggtaaaattt gtggtttgtt ggtgttaaaa cggtg
//
LOCUS       DAZGAN020000414          653 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZGAN020000414 DAZGAN020000000
VERSION     DAZGAN020000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.421"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..604)
                     /locus_tag="ACHKYO_RS27220"
                     /old_locus_tag="pgaptmp_005444"
     CDS             complement(<1..604)
                     /locus_tag="ACHKYO_RS27220"
                     /old_locus_tag="pgaptmp_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU4432062.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKASEASKSAAAAESSKSAAATSA"
BASE COUNT          112 a          207 c          175 g          159 t
ORIGIN      
        1 cggcactggt agctgccgcg ctttttgagg actctgcagc agcagcactt ttcgatgctt
       61 cactggcctt tgttgatgcc gttcctgcgc tggaagatgc tgactgagcc gacgacgcgg
      121 cctgtccggc tgacgtgctg gctgcgcgtg ctgagcctgc agcatcagtc gcatgggttg
      181 ccgcctcacg ggctgatgtg ccggcatcgc cggctgactt cttcgcggct gccgtgttct
      241 gtgccaccgc ggacgcgtta cgcgccacct cttccaccat cagctcaaaa cggcgcagtg
      301 cctctggccg ggcatcatcc tccgtcatgg caccgagaaa atcattcagc gtaccgggtc
      361 gggagtcttc atacacggtg atggtcccgg catgtgacgg cgggaatcct tccaccaaca
      421 gaataacgct gtactgaccg tactcaacgt ccatgctgta acgcccggct tcatccggat
      481 tttctgaggc caccgtgttc accaccaccg tggtgctgtt acgttttgct ttcagctgga
      541 ttgtgcagtt ctgtaccggt tttcctgtgc cgtctttcag tacacctgaa atctttactg
      601 ccatattcac cccacaaaaa agcccgcctg aaccggcggg ctgtcataac act
//
LOCUS       DAZGAN020000415          653 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZGAN020000415 DAZGAN020000000
VERSION     DAZGAN020000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.422"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>653)
                     /locus_tag="ACHKYO_RS27225"
                     /old_locus_tag="pgaptmp_005445"
     CDS             complement(<1..>653)
                     /locus_tag="ACHKYO_RS27225"
                     /old_locus_tag="pgaptmp_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4432063.1"
                     /translation="HIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGC
                     GWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKV
                     QNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLR
                     LRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIA
                     "
BASE COUNT          155 a          187 c          183 g          128 t
ORIGIN      
        1 gcaataccct gcagtttcgg gtgatcgctg agatatttca gggaggcttt gtagtcttcc
       61 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg
      121 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
      181 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa
      241 accgccggga tgccttcgtc cgggcgaccc gtacagatga actcatacgg ccccagtttg
      301 gcgatttcat ccgccagata agcggcaacc tggtaagagg cgttctgtac tttggtatag
      361 ccttcacgac cgaggcgcag gaattcatag tactgtgcaa ttacctgacc cgccgggcgg
      421 gagaagttga tggcaaaagt accaatttga ccacccaggt agtcaacgtt gaacaccagt
      481 tcctgcggca gcgcttcttc gtcacgccag ataacccagc cgcagcccag cggagccaga
      541 ccgaatttat ggcctgaagc actgatcgat ttcacacgcg gcaggcggaa gtcccagacg
      601 atatccgggg cgacgaacgg tgccaggaag ccaccgctgg cagcgtcgat gtg
//
LOCUS       DAZGAN020000416          651 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZGAN020000416 DAZGAN020000000
VERSION     DAZGAN020000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.423"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>651
                     /locus_tag="ACHKYO_RS27230"
                     /old_locus_tag="pgaptmp_005446"
     CDS             <1..>651
                     /locus_tag="ACHKYO_RS27230"
                     /old_locus_tag="pgaptmp_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4432064.1"
                     /translation="IDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVD
                     AVEAYRGSPQLMVERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDN
                     LARSQLDERIVAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQ
                     EIKAEDINWLPEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIR"
BASE COUNT          164 a          173 c          183 g          131 t
ORIGIN      
        1 atcgactgtt tacctgaagt gacgcagcag gcattacgtg aacgctatgc cctgcaactg
       61 atgacgcaaa aagccgatga atcaccggct ccggtggtga caaaggccag gcgctcacct
      121 gccgtggttg atgcggtgga ggcatatcgc ggatcacccc aactgatggt cgaacgcctc
      181 aatgccctga ctgaaaatca gcgccaggtg gctgatgcac gaatcgcgat cgtcagcgaa
      241 gttctgaaag tcgcgcaaca acctggtttc agctgtgcga aggctatccg gtttatcgtt
      301 gacaacctgg cacgttcgca gctggacgag cgcattgtgg caatggttga gacggcgaac
      361 gccaaaaagg gaaacagccg cgcgttgagt gaaatcacgc tgaaacgctg gattgcagcc
      421 tttaacaagg cgcagaacgc cgctgaacgc ctgcttttgc tggcaccggg taaacgccag
      481 gaaataaaag ccgaagatat taactggctg cccgaatttc tggcgcagta tcgccagtca
      541 aacggccgac caatgaccga ggcttacgag gattttgttg ctgaatggca gcaccggcac
      601 gctgatgagc cttatatgct cgatatcatg ccgtcttatg acaccattcg c
//
LOCUS       DAZGAN020000417          651 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZGAN020000417 DAZGAN020000000
VERSION     DAZGAN020000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.424"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>651)
                     /gene="wzy"
                     /locus_tag="ACHKYO_RS27235"
                     /old_locus_tag="pgaptmp_005447"
     CDS             complement(<1..>651)
                     /gene="wzy"
                     /locus_tag="ACHKYO_RS27235"
                     /old_locus_tag="pgaptmp_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O91 family O-antigen polymerase"
                     /protein_id="HGU4432065.1"
                     /translation="YSLFLFLFLLLPRKRIFEISLKFRSIFIFSLLPGLVIYALLIIG
                     VKLPYGILDAHNELKDSLGIYYRDYIGTVALSHLVLTVGDSTIIRFSGMLDEPGLLGT
                     ISALLLLADKLNFKHKSNYVLLLSGVISISLAFYLLILMGLIFQTRRKIISLAIVFLI
                     GIAIYHSPFYENFLQKRISDSESGLVFRDNRISDCFKEYYTAFENDSELNLFFGIGN"
BASE COUNT          265 a          115 c           84 g          187 t
ORIGIN      
        1 attaccaata ccaaaaaaaa gatttaattc actatcattt tcaaatgcag tgtaatattc
       61 tttaaaacaa tcagatattc tattatccct aaaaacaagc cccgactcac tgtcagaaat
      121 tcttttttgc aaaaaatttt cataaaaagg actatggtat atagctatgc caataaggaa
      181 cactattgct aaagaaataa ttttacgccg tgtttgaaag ataagcccca ttaaaattaa
      241 taggtaaaaa gcaagtgata tacttatcac tcctgacaat aacaatacat agtttgattt
      301 gtgtttaaag tttaatttat ccgctaacag tagaagtgct gaaattgttc ctaacaaccc
      361 aggttcatca agcattccag aaaatcgtat aatagttgag tcccctactg ttaaaactaa
      421 atgcgacaaa gctacagttc caatataatc acgataatag attcccaatg aatccttaag
      481 ctcattgtga gcatcaagaa taccatatgg cagtttaact cctataatta ataacgcata
      541 gataaccaac cccggtaata aagaaaaaat aaatatagaa cggaacttca aagatatctc
      601 aaatattctt tttctaggta gcaataggaa cagaaataaa aacaaagaat a
//
LOCUS       DAZGAN020000418          647 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZGAN020000418 DAZGAN020000000
VERSION     DAZGAN020000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.425"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..570
                     /locus_tag="ACHKYO_RS27240"
                     /old_locus_tag="pgaptmp_005448"
     CDS             <1..570
                     /locus_tag="ACHKYO_RS27240"
                     /old_locus_tag="pgaptmp_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HGU4432066.1"
                     /translation="DNMRAVSVLNLGTIDEPGHADNTATLKLKKTAPFSALLSVNGER
                     NSQKSLAEWIEDWADYLVGFDANGDAIQATKAAAAIRKITIEANQTADFEDNDFSGKR
                     SLMESVEAKTKDIMPVAFEFKCVPFEGLKERPFKLRLSIITGDRPVLVLRIIQLEAVQ
                     EDMANEFRDLLVEKFKDSKVETFIGTFTA"
BASE COUNT          182 a          146 c          156 g          163 t
ORIGIN      
        1 gataatatgc gtgccgtcag tgtgcttaac ctgggtacta ttgatgaacc aggtcacgca
       61 gataacaccg ccactctcaa actgaaaaag acagcaccgt tctctgccct gttgtctgtt
      121 aacggcgagc gtaactccca gaaatcactg gcagaatgga ttgaagactg ggccgactac
      181 cttgtgggct ttgatgctaa tggtgacgcc attcaggcaa caaaagcggc tgcggcaatc
      241 cgtaaaatca cgattgaagc aaaccagacc gctgattttg aagataatga cttcagcggc
      301 aaacgctccc tgatggaatc tgtcgaagcg aagaccaaag acattatgcc agtggcattt
      361 gaatttaaat gcgttccgtt tgaaggtctg aaagaacgtc cgtttaaatt acgcctcagc
      421 attatcactg gcgatcgtcc tgtactggtt ctgcgcatta ttcagctgga agcggtgcag
      481 gaagatatgg ctaacgaatt tcgtgatctg cttgttgaga aattcaaaga cagcaaagta
      541 gaaaccttta ttggtacttt caccgcctga tttcattact gcaaatgccc ctgcgggggc
      601 atttatggaa acgtaattaa ctcaataatc accggatggt gagggct
//
LOCUS       DAZGAN020000419          640 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZGAN020000419 DAZGAN020000000
VERSION     DAZGAN020000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.426"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>640)
                     /locus_tag="ACHKYO_RS27245"
                     /old_locus_tag="pgaptmp_005449"
     CDS             complement(<1..>640)
                     /locus_tag="ACHKYO_RS27245"
                     /old_locus_tag="pgaptmp_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4432067.1"
                     /translation="ARASNDAELLGYGQGERVRERMRELQQIRDGYRQKDADLQSQYQ
                     TGDISEDFYRQALAQNAQYLSERLKDQEAFYAESDAQRADWQKGLQEGLSNWVDSASD
                     YASQAAQLATDGISGMVNNITEMLNGNKVEWRSWAASILQEISKVLMNAAIVNGIKMA
                     ANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSANLSAYSNSIVDTPTYFAFA"
BASE COUNT          127 a          206 c          137 g          170 t
ORIGIN      
        1 tttgcaaagg caaagtacgt gggcgtgtcc acaatactgt tactgtatgc actcaggttt
       61 gccgaggtat aaacgccgcc ttttgcattt gccaccgcgc cacccagcca gttgcctata
      121 ctgccgaaaa atcctcctgc accggacata ctgtttgccg ccatcttaat gccgttgaca
      181 atggccgcat tcataagaac ttttgatatt tcctgcagga ttgatgcagc ccagctgcgc
      241 cattccactt tatttccgtt cagcatctcc gtgatgttat tcaccatccc tgagataccg
      301 tctgtcgcaa gctgtgctgc ctgtgaagcg taatctgatg cgctgtccac ccagttactt
      361 agcccttcct gcagcccttt ctgccagtca gcacgctgcg catccgattc ggcataaaaa
      421 gcctcctggt ctttaaggcg ttcactcaga tactgcgcat tctgcgccag cgcctgtctg
      481 taaaaatcct cactgatatc ccctgtctga tactgagact gcaggtccgc atccttctgg
      541 cggtagccgt cacgaatctg ctgcagctcc cgcatccgtt cacggacccg ctcaccctgc
      601 ccgtacccca gcagctcagc gtcatttgat gcccttgcgt
//
LOCUS       DAZGAN020000420          615 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZGAN020000420 DAZGAN020000000
VERSION     DAZGAN020000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.427"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            23..>615
                     /locus_tag="ACHKYO_RS27250"
                     /old_locus_tag="pgaptmp_005450"
     CDS             23..>615
                     /locus_tag="ACHKYO_RS27250"
                     /old_locus_tag="pgaptmp_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HGU4432068.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGVLLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTF"
BASE COUNT          145 a          150 c          175 g          145 t
ORIGIN      
        1 ttttttatta tcaggaggca gaatgtctgc tttgtatgaa cgctcacagc tgacgcaggt
       61 gatgatttca tctgccccgg cgactgctga aactatggat aaggcggaat atctgcgcct
      121 ggactgcacc atcaaggaag tccagttcac cgccggtcag aaacaggata ttgatgtgac
      181 cacgctctgc tccacagagc aggagaacat caacggtctg ggggcgtcgt ccgagatttc
      241 catgtcgggt aatttttatc tgaatcaggc ccagaacgcc ctgcgtgatg cctatgacaa
      301 tgacgcgttg tatgcgttta aggtgcagtt tccgtccggt aagggcttta aattcctggc
      361 ggaagtgcgt cagcacacct ggtcatccgg taccaacggc gtggtggctg caacgttctc
      421 actgcgtctg aaaggcaaac cggtgtcctt tgtggtaccg ctggcgtttg tgaaaaatct
      481 ggataagaca cttaccgtga ataccggtgt gctgctgaca atgtcagtca gtgccaacgg
      541 gggaacgccg ccgtataaat acgcctggaa gaaggatggt cagccggttg acgggcagac
      601 gacagacacc ttcag
//
LOCUS       DAZGAN020000421          612 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZGAN020000421 DAZGAN020000000
VERSION     DAZGAN020000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.428"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..366
                     /locus_tag="ACHKYO_RS27255"
                     /old_locus_tag="pgaptmp_005451"
     CDS             <1..366
                     /locus_tag="ACHKYO_RS27255"
                     /old_locus_tag="pgaptmp_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HGU4432069.1"
                     /translation="AHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVS
                     GDPHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFK
                     KKIKKVIRDVKVKLGLKSK"
     gene            391..>612
                     /locus_tag="ACHKYO_RS27260"
                     /old_locus_tag="pgaptmp_005452"
     CDS             391..>612
                     /locus_tag="ACHKYO_RS27260"
                     /old_locus_tag="pgaptmp_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU4432070.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIADYLDKEYIEL"
BASE COUNT          234 a           79 c          115 g          184 t
ORIGIN      
        1 gctcattatt atattatgct cattgagtat attgaaggtg tcgggttaaa tgagtatttg
       61 gaaatttctg aagatctgaa ggatcaatta tcagaatcga taaaagagtt gcatcagcat
      121 ggaatggtgt ctggcgatcc tcataaagga aattttatag tatccgaaaa aggtttaaga
      181 ctaattgacc tttcaggtaa aaaaacaacc gctgttttaa aagcaaaaga tcgcattgat
      241 cttgaacggc actataatat aaaaaatgaa ttgaaagact ttggctatac atatcttata
      301 ttcaaaaaga aaattaaaaa agtaatcagg gatgtaaaag tcaaactagg attgaagagc
      361 aaataatgat tatgatgaag ggtaattaaa ttggatttta aacatcttac tcaatttaaa
      421 gatataattg aactggacaa gcgccccgtt aaacttgatg aacgggaaac gtttaatgtc
      481 tcatggggta ttgatgagaa ctaccaggtt ggggctgcga tttcaattgc ttcaattctt
      541 gaaaataata aacaaaacaa atttaccttt cacataatcg ctgattactt agacaaagag
      601 tatattgaat ta
//
LOCUS       DAZGAN020000422          599 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZGAN020000422 DAZGAN020000000
VERSION     DAZGAN020000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.429"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>599
                     /locus_tag="ACHKYO_RS27265"
                     /old_locus_tag="pgaptmp_005453"
     CDS             <1..>599
                     /locus_tag="ACHKYO_RS27265"
                     /old_locus_tag="pgaptmp_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4432071.1"
                     /translation="QARESAHQYALTIDEIAHKTPSMSLPEASDNEGRTREALTEQNR
                     LIDEQASRVKSLQEKIAGYQYVLANPGWTTGNGFMINHLTSVKTVTEGLAQATEQFAV
                     EQSRLAQMQEKAQSIQDVLAGLEERRVVLIRQQAAEQNKAYQSLLIMNGQHTEFNRLL
                     GLGNELLQQRQGLASVPLRLPQATLDDKQQGALNNTERQL"
BASE COUNT          161 a          147 c          180 g          111 t
ORIGIN      
        1 caggccaggg agtctgcgca ccagtatgca ctgacgattg atgaaatcgc gcataaaacg
       61 ccatcaatgt ctctgcctga agcctcagat aatgaagggc gaacacggga ggcgctgaca
      121 gagcagaacc ggctgattga tgagcaggcc agccgggtga aatccctgca ggaaaaaatc
      181 gccgggtatc agtatgtgct ggctaatccg ggctggacga ccggtaacgg cttcatgata
      241 aaccatctga catcggtgaa aactgtaacg gaagggcttg ctcaggcaac agagcagttt
      301 gctgttgagc agtcccgtct ggcacagatg caggaaaaag cgcagtccat tcaggatgtg
      361 cttgccggac tggaagagcg ccgtgttgtg ttaattcgtc agcaggcggc agagcagaat
      421 aaagcgtatc agtcactgct gatcatgaac ggtcagcata cggaattcaa ccgcctgctg
      481 gggctgggta atgaactgct gcaacagcgt cagggactgg cgagtgtacc gctgcgactg
      541 ccacaggcca ctctggatga taaacagcag ggcgccctga ataacacaga gcgtcaact
//
LOCUS       DAZGAN020000423          599 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZGAN020000423 DAZGAN020000000
VERSION     DAZGAN020000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.430"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>599
                     /locus_tag="ACHKYO_RS27270"
                     /old_locus_tag="pgaptmp_005454"
     CDS             <1..>599
                     /locus_tag="ACHKYO_RS27270"
                     /old_locus_tag="pgaptmp_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4432072.1"
                     /translation="QARESARQYALTIDEIAHKTPSMSLPEASDHEGRTREALTEQNR
                     LIDEQASRVKSLQEKIAGYQYVLANPGWTTGNGFMINHLTSVKTVTEELAQATEQFAV
                     EQSRLAQMQEKAQSIQDVLAGLEERRVVLIRQQAAEQNKAYQSLLVMNGQHTEFNRLL
                     GLGNELLQQRQGLASVPLRLPQATLDDKQQSALNNTERQL"
BASE COUNT          161 a          148 c          179 g          111 t
ORIGIN      
        1 caggccaggg agtctgcgcg ccagtatgca ctgacgattg atgaaatcgc gcataaaacg
       61 ccatcaatgt ctctgcctga agcctcagat catgaagggc gaacacggga ggcgctgaca
      121 gagcagaacc ggctgattga tgagcaggcc agccgggtga aatccctgca ggaaaaaatc
      181 gccgggtatc agtatgtgct ggctaatccg ggctggacga ccggtaacgg cttcatgata
      241 aaccatctga catcggtgaa aactgtaacg gaagagcttg ctcaggcaac agagcagttt
      301 gctgttgagc agtcccgtct ggcacagatg caggaaaaag cgcagtccat tcaggatgtg
      361 cttgccggac tggaagagcg ccgtgttgtg ttaattcgtc agcaggcagc agagcagaat
      421 aaagcgtatc agtcactgct ggtcatgaac ggtcagcata cggaattcaa ccgcctgctg
      481 gggctgggta atgaactgct gcaacagcgt cagggactgg cgagtgtacc gctgcgactg
      541 ccacaggcca ctctggatga taaacagcag agcgccctga ataacacaga gcgtcaact
//
LOCUS       DAZGAN020000424          586 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZGAN020000424 DAZGAN020000000
VERSION     DAZGAN020000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.431"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..416
                     /locus_tag="ACHKYO_RS27275"
                     /old_locus_tag="pgaptmp_005455"
                     /pseudo
     CDS             1..416
                     /locus_tag="ACHKYO_RS27275"
                     /old_locus_tag="pgaptmp_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(455..>586)
                     /locus_tag="ACHKYO_RS27280"
                     /old_locus_tag="pgaptmp_005456"
     CDS             complement(455..>586)
                     /locus_tag="ACHKYO_RS27280"
                     /old_locus_tag="pgaptmp_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432073.1"
                     /translation="DAYVDEVLARMVGRSPAENSTENEPQPVEQPEPVQPGTDGTMM"
BASE COUNT          162 a          139 c          127 g          158 t
ORIGIN      
        1 gagtgaaaac gatcataaaa tgatcatttt attgactttt tatccagtga aggtaggatc
       61 gcgcgtatat gtagtatgtt tcaaatcaac aggatactac ataaaaggga aacccagcat
      121 agctggaact atgctgggtc aatctgtaaa aacttgcatc aatgctttac ttcgagctcg
      181 tggcgtatcc tatgatattc cccggtctcc tgtaaagcag agatgcataa aaaggagatc
      241 tttatcatgg ataaagagcc tgtttgccct gcctgtaaga ctcccttaca gctcatctat
      301 cgccgttctc gatctcaaaa tggcaagaca tacaaaccaa aaatgcttcc tgtttcagga
      361 tgctgatatg tcctgcctgt tctccccgga gggaaaatac acaaaacgtg cattaacaaa
      421 ttcacccggc agggaaacct gccggtcggt attgttacat catagtaccg tcagttccgg
      481 gctggaccgg ctctggttgc tctacgggtt gaggctcatt ttccgtgctg ttttcagccg
      541 gagaacgacc aaccattcgg gccagcactt catcaacata tgcgtc
//
LOCUS       DAZGAN020000425          584 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZGAN020000425 DAZGAN020000000
VERSION     DAZGAN020000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.432"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..327
                     /locus_tag="ACHKYO_RS27285"
                     /old_locus_tag="pgaptmp_005457"
     CDS             <1..327
                     /locus_tag="ACHKYO_RS27285"
                     /old_locus_tag="pgaptmp_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HGU4432074.1"
                     /translation="RKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDRAMLLGA
                     QALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQDHGVIAV
                     DTAVKL"
     gene            383..>584
                     /locus_tag="ACHKYO_RS27290"
                     /old_locus_tag="pgaptmp_005458"
     CDS             383..>584
                     /locus_tag="ACHKYO_RS27290"
                     /old_locus_tag="pgaptmp_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432075.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATV"
BASE COUNT          142 a          137 c          164 g          141 t
ORIGIN      
        1 cgtaagtatg cgggtatgcc gatccgtttc tatcaggggt caaaggttct ggtatcagag
       61 aataacctga cggcaaccac gaaagaggtc gctgctgcaa ccaatattga ccgcgccatg
      121 ttactggggg ctcaggcgct ggcaaatgct tacggtcaga aggcgggcgg tcacttcaac
      181 atggttgaga agaaaacgga tatggataac cgtactgaga tagcaatcag ctggatcaac
      241 ggtctgaaaa aaatccgttt ccccgagaag agcggcaaga tgcaggatca cggcgtgatt
      301 gccgttgata cagcagtgaa gctctgattt tttcctttcc ccatgccggg ttttcgcccg
      361 gctttttcag gagtcattaa ttatggcaaa gactatcctt gccccgtcac tgagtgaacg
      421 ggtctatacg ggtacgcacg gtaatgagtc ggtggcagaa ggcgtattta cggtgaatgc
      481 tgcggaagcg gacagtgtta ttcatcttct ctcactgcca gtgggcatcc gtatcaactc
      541 actccagctg gtttcaacgg gtggtctggg tactgcaacc gtca
//
LOCUS       DAZGAN020000426          583 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZGAN020000426 DAZGAN020000000
VERSION     DAZGAN020000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.433"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>583
                     /locus_tag="ACHKYO_RS27295"
                     /old_locus_tag="pgaptmp_005459"
     CDS             <1..>583
                     /locus_tag="ACHKYO_RS27295"
                     /old_locus_tag="pgaptmp_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HGU4432076.1"
                     /translation="MKKIECACNFLMDKDAQGYIDLSDLDLTSCHFKGDVISKVSFLS
                     SNLQHVTFECKEIGDSNFTTAIVDNVIFRCRRLHNVIFIKASGECVDFSKNILDTVDF
                     SQSQLTQSNFRECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNMTAVMFDHVRI
                     STGNFKDCITEQLELTIDYSDIFWNEDHDGYIN"
BASE COUNT          192 a           83 c          109 g          199 t
ORIGIN      
        1 aaatgaaaaa aattgaatgc gcttgcaatt ttctgatgga taaagatgcg caggggtata
       61 tcgacctgtc tgatttggat ttaacaagtt gtcattttaa aggtgacgtt atatcgaagg
      121 tgtctttttt atcatcaaat ctacaacatg taacattcga atgtaaagaa attggggatt
      181 ccaattttac tactgcaata gttgataatg tcatatttag atgtcgacgt ttacacaatg
      241 tgatttttat caaagcgagt ggtgaatgtg tcgatttcag caaaaatatt cttgatacag
      301 ttgacttctc gcagagtcaa cttactcaga gtaattttcg cgaatgtcag attagaaatt
      361 caaacttcga taattgttat ctttacgctt cgcacttcac cagagcagag tttctgtctg
      421 ccaaagaaat atcatttatt aaatcgaata tgacagctgt tatgtttgat catgtgcgaa
      481 tatcgacagg gaattttaaa gattgcatta cagaacaatt ggaattaact attgattatt
      541 cagatatatt ttggaatgaa gatcacgatg gttatatcaa taa
//
LOCUS       DAZGAN020000427          583 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZGAN020000427 DAZGAN020000000
VERSION     DAZGAN020000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.434"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>583
                     /locus_tag="ACHKYO_RS27300"
                     /old_locus_tag="pgaptmp_005460"
     CDS             <1..>583
                     /locus_tag="ACHKYO_RS27300"
                     /old_locus_tag="pgaptmp_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4432077.1"
                     /translation="VLADLQRTFEGKIAAQAQTFEEKLASLSVVLQKCVTGDDVRPML
                     EQMVKEAVSHIPVPRDGRDYDPDVLQKAVNDAVGKIPVPANGKSITPDDVRPMIEQMV
                     KEAVSHIPVPRDGRDYDPDVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVKEAV
                     SHIPVPRDGRDYDPEVLQKAVNDAVANIPQPADG"
BASE COUNT          139 a          128 c          188 g          128 t
ORIGIN      
        1 gtgctggcgg atttacagcg cacatttgag gggaaaatag ccgcgcaggc acaaacgttt
       61 gaagaaaaac tggcttccct gtctgtggta ttacagaagt gcgtgacggg cgatgatgtg
      121 cgtccgatgc ttgagcagat ggtgaaggag gcggtaagcc atatccctgt tccgcgcgac
      181 ggtcgtgact atgatcccga tgttctgcag aaggcggtga atgatgctgt cgggaaaata
      241 ccggtaccgg cgaacggtaa aagtattact cccgatgatg tgcgtccgat gattgagcag
      301 atggtgaagg aggctgtaag ccatattcct gttccgcgcg acggtcgtga ctatgatccc
      361 gatgttctgc agaaggcggt gaatgatgcg gtcgcaaata ttccgcagcc ggcggacggt
      421 aaaagtctca ccccggatga tgtgcgtccg atgcttgaac agatggtgaa agaggcagtg
      481 agccatattc ctgttccgcg tgatggtcgt gactacgatc cggaagtact gcagaaggcg
      541 gtgaatgatg cagtcgcaaa tattccgcag ccggcggacg gta
//
LOCUS       DAZGAN020000428          580 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZGAN020000428 DAZGAN020000000
VERSION     DAZGAN020000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.435"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            111..>580
                     /locus_tag="ACHKYO_RS27305"
                     /old_locus_tag="pgaptmp_005461"
     CDS             111..>580
                     /locus_tag="ACHKYO_RS27305"
                     /old_locus_tag="pgaptmp_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HGU4432078.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIEGHPAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDESTGEPVELRMLAITGDPGLT"
BASE COUNT          129 a          165 c          159 g          127 t
ORIGIN      
        1 atttaacggg ctttaaaatc tgcgtgaagc gcctttgtat ttttcccctg gaagcacttc
       61 ctgaaatccc gcaaaacccg ccctgaaaat tcccggctta tgctgccgga atgaagacga
      121 aaaacacgcc caaacttgct tatgccattc tgaatgccat cagcctgtcc gcagacgggg
      181 acggtgactg gtgtcagatc atgccagccg gtcgggtaaa ggcccgtgac ggtcgcccgg
      241 aaaaaccggc cgagggctgg ctgatcaacc atgcagccgt tgagcgcatg gtctcccgtg
      301 ttgtggcgct caatcagccg gtgaaaattg actacaacca ccagaccctg attgagggac
      361 atccggcacc ggctgccggt tttgtgatgg cctcaccgga gaattttcgc ttcagtgaag
      421 agcgaggttt cgaggtgcgc ccgaagtgga accctccggc ccttgaacat ctgcgtaata
      481 acgaattccc ctggttttca ccggtgattg gctatgacga gagcaccggc gaacctgtcg
      541 aacttcggat gctggctatt accggtgacc ccggtctgac
//
LOCUS       DAZGAN020000429          579 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZGAN020000429 DAZGAN020000000
VERSION     DAZGAN020000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.436"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..183
                     /locus_tag="ACHKYO_RS27310"
                     /old_locus_tag="pgaptmp_005462"
     CDS             <1..183
                     /locus_tag="ACHKYO_RS27310"
                     /old_locus_tag="pgaptmp_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432079.1"
                     /translation="LGLQGGEQTYRYVPNPLRWIDPLGLNKGASLSKMMNSSSDLMGL
                     RRQPQNFWRLYRGKDI"
     gene            216..476
                     /locus_tag="ACHKYO_RS27315"
                     /old_locus_tag="pgaptmp_005463"
     CDS             216..476
                     /locus_tag="ACHKYO_RS27315"
                     /old_locus_tag="pgaptmp_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432080.1"
                     /translation="MLEHFGAEASVLDMTIIVRSNPSKAAILEEFLHGTQEKLGIAEK
                     LGRYGLGSAETHVKDFMIRHKKMLGLSDEDVAILKILKDKGL"
BASE COUNT          189 a           92 c          138 g          160 t
ORIGIN      
        1 ctggggttgc agggagggga gcagacttac cggtatgtgc cgaatccttt aagatggata
       61 gatcccttag gattaaataa aggagcttca ttatctaaaa tgatgaatag ctccagtgat
      121 ctcatggggt tgagaaggca gccccagaac ttctggcggc tatatcgcgg aaaagacatt
      181 taataatagc attaccaggc tcagatgaat tcagaatgct tgaacatttt ggagccgaag
      241 cttcggtatt agacatgacg attattgttc gttcaaatcc gagtaaagcc gctattcttg
      301 aagagttttt acatggtaca caagagaaac taggaatagc tgaaaaatta ggacgttatg
      361 gactagggag tgcagaaacg cacgttaagg atttcatgat aagacacaaa aaaatgcttg
      421 ggttatctga tgaggatgta gcaatactca aaatattgaa agacaagggg ttgtgatgaa
      481 tataattctg ccgataaaag atatatttat tattgcggga aacatagttt ttagcgtttt
      541 atacaatgaa catcttgagt tcccatgccg ctgccaatt
//
LOCUS       DAZGAN020000430          574 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZGAN020000430 DAZGAN020000000
VERSION     DAZGAN020000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.437"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(354..>574)
                     /locus_tag="ACHKYO_RS27320"
                     /old_locus_tag="pgaptmp_005464"
     CDS             complement(354..>574)
                     /locus_tag="ACHKYO_RS27320"
                     /old_locus_tag="pgaptmp_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HGU4432081.1"
                     /translation="GKLPEAFFWTDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHV
                     RQRTMKKELEKLTSADEILAYRVGWGDP"
BASE COUNT          155 a          150 c           95 g          174 t
ORIGIN      
        1 ctcataaata aatatgatta attacggctt cattcaggat attcccaaaa taaatccaaa
       61 aattaattta ataatttagg atttgtctta cattattctt ggtgtagtca ctcagaacca
      121 cgactacacc accggaacag gcatcagaaa aaactcactc tcatccggca tgctgtgtca
      181 catctgctta cactgtaatt tatgagaaaa atctcaatag cagtaaaaag taactgttca
      241 gttaaagtgt aatatttacc taagtaactt ttcctgtgta ttaccgtgaa ggagcataat
      301 tggttattgg gcgttctcct tccataaaaa tacgcagtgt cctcctttcc agattacgga
      361 tcaccccagc caaccctgta tgccagtatc tcatccgcac tggtcagctt ttccagctct
      421 ttcttcatgg tgcgctgccg gacgtggatt tccatccctt tggtgaacat cgcctgctct
      481 gccgcttcac tcagcgctat aagctcttct gctgtcacct ccacatcatt gttttccgca
      541 tccgtccaga aaaacgcctc cggcagtttc cccg
//
LOCUS       DAZGAN020000431          571 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZGAN020000431 DAZGAN020000000
VERSION     DAZGAN020000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.438"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            282..443
                     /gene="rhuM"
                     /locus_tag="ACHKYO_RS27325"
                     /old_locus_tag="pgaptmp_005465"
                     /pseudo
     CDS             282..443
                     /gene="rhuM"
                     /locus_tag="ACHKYO_RS27325"
                     /old_locus_tag="pgaptmp_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774140.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT          154 a          138 c           96 g          183 t
ORIGIN      
        1 aatgatatta tagccagaca ctatccggaa atgttacctt cacaatagag tactaagaat
       61 tgattcaatt atgtatttta gcttcattcc ttcatgcaaa atcatttggg ggttcttatg
      121 ggggttactc atttatccaa cacatacaaa ttatttaaga tcaatgcatt aatacttata
      181 ttcgactcct gtgatctccg ccaaaatgcc tctcctgacg ttctccaacc ccttgtaata
      241 ctgtcaatcc agatattttc tactgctcaa tattcaatcg gcttcaggaa tacctggtct
      301 aaggttttgt gatggacgat gagcggctga agaatccgcc tgtcggttct tctgtcctcg
      361 aacgcatccg cgatattcac gccagtatgc gccgtgttta tttgcaggtg atttgccccc
      421 ctgctgactc tcagccaact gtatcgccat taaggtgaag atgttgtttt actcgcccca
      481 gaacaccctt ttttatacga ctattctatt tctttaaccg ctggcgaaaa aaactgacta
      541 taaacttcac caataatcag actgccatca a
//
LOCUS       DAZGAN020000432          567 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZGAN020000432 DAZGAN020000000
VERSION     DAZGAN020000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.439"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>567
                     /locus_tag="ACHKYO_RS27330"
                     /old_locus_tag="pgaptmp_005466"
     CDS             <1..>567
                     /locus_tag="ACHKYO_RS27330"
                     /old_locus_tag="pgaptmp_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4432082.1"
                     /translation="DLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVV
                     GILPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRD
                     LRSAFTALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPVKPNGKH
                     YSPAEFRKKRLAEEDTEIEWLSDVELRD"
BASE COUNT          143 a          148 c          164 g          112 t
ORIGIN      
        1 tgacctggct gaaaatgttt tcgccgtagc cggtgccata cagcacggca ttcgccatca
       61 cggcaaaccg tttctgtatt actcggataa cggctccggg gaaaccgccg acatcctgga
      121 taaggaggtt gtggggatac tgccgcgact ggggattaat cacccgaccg ggattgccgg
      181 taatccgcag ggacgaggca ttatcgaacg gcttaaccgc acattaccga tgcgcatagc
      241 ccgtaaatac cgcacctatt tcgggaaagg tgcagatcgc gagacgttac gcaaaaccaa
      301 ccgcgattta cgctcggcat ttactgccct gcaacagggc aaacggctga acgcccggca
      361 gcagtcagcg atgcgtgatt taccgtcctg gtctgaactg attgatgcca ttcgtgatgg
      421 tgttgagtgg tacaacaacc ggccgcacga tgaattaccg gtgaagccga acggcaagca
      481 ttacagcccg gcggagttca gaaaaaaacg cctggcggaa gaggacacgg aaattgaatg
      541 gctgtccgat gttgagctgc gggatat
//
LOCUS       DAZGAN020000433          565 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZGAN020000433 DAZGAN020000000
VERSION     DAZGAN020000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.440"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>565
                     /locus_tag="ACHKYO_RS27335"
                     /old_locus_tag="pgaptmp_005467"
     CDS             <1..>565
                     /locus_tag="ACHKYO_RS27335"
                     /old_locus_tag="pgaptmp_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
                     /protein_id="HGU4432083.1"
                     /translation="GGVNENIFNGYFSDVELSKGNSALTEKVNRDYFSKPVDDYFLNN
                     HMISYDPSKNQWKNLGTTPFPGTAGSSVIFAEQQIYILGGERKPGLRSVRSWTGELSH
                     DRIKWSELPPVASPEGVSGAYASVIDGNIFLAGGAYFPGAAEKYSNGEYWSHKGLDKA
                     YSKEIYQLIKNDWKKVGSLPEGLAYGVS"
BASE COUNT          168 a           94 c          143 g          160 t
ORIGIN      
        1 ggtggtgtaa atgagaatat ttttaatgga tacttttcag atgtcgagtt atcaaaaggg
       61 aattccgcgc tgacagagaa agtcaacagg gattatttta gtaaacctgt tgatgattat
      121 tttttaaata atcatatgat ttcttatgac ccgtcaaaaa accagtggaa gaacctggga
      181 acgactccat ttccgggaac tgctggttca tcggtgattt ttgcagaaca gcaaatttat
      241 atactgggag gggagagaaa accgggcctt cgttctgtta gatcctggac gggggagctt
      301 tcgcatgatc gaattaaatg gtctgaactt ccaccagtag cttcacccga gggggtgtca
      361 ggagcatatg cctctgttat tgatggaaat atctttctgg ccggtggtgc atatttccca
      421 ggggcggcag aaaagtatag caatggtgaa tactggtctc ataaaggact ggataaagcc
      481 tattcaaaag agatatatca gctaataaaa aatgactgga aaaaagttgg ttctttgccg
      541 gaaggacttg cttatggtgt aagcc
//
LOCUS       DAZGAN020000434          555 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZGAN020000434 DAZGAN020000000
VERSION     DAZGAN020000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.441"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>555)
                     /locus_tag="ACHKYO_RS27340"
                     /old_locus_tag="pgaptmp_005468"
     CDS             complement(<1..>555)
                     /locus_tag="ACHKYO_RS27340"
                     /old_locus_tag="pgaptmp_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGU4432084.1"
                     /translation="KPVPDMPSQLAARLDEVWAAIKTGLVRGLSVGFRPHEYTFLDGG
                     GLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFSAASGNRKPVVKIASSAGAAAQS
                     TTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMTKAAEEGRTLDVEEEEHYDNTAA
                     EIRQVDAHLKRLRELEAGKAATAQP"
BASE COUNT          103 a          175 c          151 g          126 t
ORIGIN      
        1 ggctgcgccg tggcggcctt accggcttcc agttcacgca ggcgcttcag gtgcgcatcc
       61 acctgacgga tttccgctgc ggtgttgtcg taatgctcct cctcctccac atccagcgtg
      121 cgcccttcct ctgcggcttt ggtcatgacc tcctcaaggg aggctgccag cgctgcacgc
      181 ttgttttcaa aacttttaat ctgttcgcca atattcatta tggtcttttc cttatgaaaa
      241 acggttgttg actgtgccgc agcgccggca gaagatgcga ttttcaccac cggtttccgg
      301 ttgccggacg cggcagaaaa cgggcggtcg taagatttaa tggtccggat ggtgcattcc
      361 gcattcgcgg gcacggtaac ggcagacacc tccatcagct cccagcgcag aaaatgcagt
      421 ccacctccgt ccagaaaggt gtattcatgg ggacggaagc ccacggacag ccccctgacc
      481 agcccggtct taatggccgc ccagacctca tccagccggg cagccagttg cgacggcata
      541 tccggtacgg gcttc
//
LOCUS       DAZGAN020000435          555 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZGAN020000435 DAZGAN020000000
VERSION     DAZGAN020000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.442"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>555)
                     /locus_tag="ACHKYO_RS27345"
                     /old_locus_tag="pgaptmp_005469"
     CDS             complement(<1..>555)
                     /locus_tag="ACHKYO_RS27345"
                     /old_locus_tag="pgaptmp_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGU4432085.1"
                     /translation="KPVPDMPSQLAARLDEAWAAIKTGLVRGLSVGFRPHEYTFLDGG
                     GLHFLRWELMEVSAVTVPANAECTIRTIKSYDRQFSAASGNRKPMVKIASSAGAAAQS
                     TTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMTKAAEEGRTLDVEEEEHYDNTAA
                     EIRQVDAHLKRLRELEAGKAATAQP"
BASE COUNT          101 a          172 c          149 g          133 t
ORIGIN      
        1 ggctgcgccg tggcggcctt accggcttcc agttcacgca ggcgcttcag gtgcgcatcc
       61 acctgacgta tttccgctgc ggtgttgtcg taatgctctt cctcctccac atccagcgtt
      121 cgcccttcct ctgcggcttt ggtcatgacc tcctcaaggg aggctgccag tgctgcacgc
      181 ttgttttcaa aacttttaat ctgttcgcca atattcatta tggtcttttc cttatgaaaa
      241 acggttgttg actgtgccgc agcgccggca gaagatgcga ttttcaccat cggtttccgg
      301 ttgccggacg cggcagaaaa ctggcggtcg taagatttaa tggtccggat ggtgcattcc
      361 gcattcgcgg gcacggtgac ggcagacacc tccatcagct cccagcgcag aaaatgcagt
      421 ccgcctccgt ccagaaaggt gtattcatga ggacggaagc ccacggacag ccccctgacc
      481 agcccggtct taatggccgc ccaggcctca tccagcctgg cagccagttg cgatggcata
      541 tccggcacgg gcttc
//
LOCUS       DAZGAN020000436          547 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZGAN020000436 DAZGAN020000000
VERSION     DAZGAN020000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.443"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>547
                     /locus_tag="ACHKYO_RS27350"
                     /old_locus_tag="pgaptmp_005470"
     CDS             <1..>547
                     /locus_tag="ACHKYO_RS27350"
                     /old_locus_tag="pgaptmp_005470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HGU4432086.1"
                     /translation="KGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIG
                     RFCSIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPII
                     GNDVWIGNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVID
                     ELLRIKWWNYNYSDLPDNNKC"
BASE COUNT          176 a           82 c          115 g          174 t
ORIGIN      
        1 caaaaggtga caagataaca attgaagata atgttattgc tgaagaatat tcaacaatgc
       61 ctgtgaaaaa tttctccagt gtcggcgctt tttcatttcc gacctgccat ttttcaggga
      121 atatacgtat aggtcgattt tgtagtattg catcaaatgt aaaaataatg ggtggaaatc
      181 atcctttgaa tcgttttact actcacatga tgacttataa cggtgaattt gataagtttg
      241 ctatgagtga atttgaacgt agctggacat tgaaaccatt cattaccaaa cctgaaaatc
      301 cgattatagg aaatgatgtc tggataggaa atgatgttgt attgaaaggt ggcatcgcaa
      361 taggtgatgg tgctgttatt gctgccaatt ccgttgttac caaagatgtg cccccatatg
      421 ccatagttgc aggagttcct gcaaaaataa tcagatttag gtttgattca aatgttattg
      481 atgaactgtt gagaataaaa tggtggaatt ataactactc agatcttccg gataataaca
      541 agtgtga
//
LOCUS       DAZGAN020000437          542 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZGAN020000437 DAZGAN020000000
VERSION     DAZGAN020000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.444"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>542
                     /locus_tag="ACHKYO_RS27355"
                     /old_locus_tag="pgaptmp_005471"
     CDS             <1..>542
                     /locus_tag="ACHKYO_RS27355"
                     /old_locus_tag="pgaptmp_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4432087.1"
                     /translation="RGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEIRYRYD
                     SDGRVTEQLNPAGLSYTYQYEKDRITITDSLDRREVLHTQGEAGLKRVVKKEHADGSV
                     TQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHNQLTSAT
                     GPDGLELRREYDESGRLIQET"
BASE COUNT          138 a          153 c          173 g           78 t
ORIGIN      
        1 cgcggcgaac tggcggcagt gtatgaccgc agcaatacac aggtgcgcag ctttacttac
       61 gatgataaat accggggccg gatggtggcg caccgtcaca cgggccgacc ggaaatccgt
      121 taccgttacg acagcgacgg gcgggtgaca gaacagctaa acccggcagg cttaagctac
      181 acgtatcagt atgagaaaga ccgcatcacc atcaccgaca gcctggaccg ccgtgaagtg
      241 ctgcacacgc agggcgaagc cgggctgaag cgggtggtga aaaaggaaca cgcggacggc
      301 agcgtcacgc agagtcagtt tgacgccgtg ggcaggctca gggcacagac ggatgccgca
      361 ggcaggacaa cagagtacag cccggatgtg gtgacgggcc tcatcacgcg cataaccacg
      421 ccggatggca gggcatcggc gttttactat aaccaccaca accagttaac gtcagccacc
      481 gggcctgacg ggctggaatt gcgccgggaa tatgatgaat ccggccgtct gattcaggaa
      541 ac
//
LOCUS       DAZGAN020000438          542 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZGAN020000438 DAZGAN020000000
VERSION     DAZGAN020000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.445"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>542
                     /locus_tag="ACHKYO_RS27360"
                     /old_locus_tag="pgaptmp_005472"
     CDS             <1..>542
                     /locus_tag="ACHKYO_RS27360"
                     /old_locus_tag="pgaptmp_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4432088.1"
                     /translation="RGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEICYRYD
                     SDGRVTEQLNPAGLSYTYQYEKDHITITDSLNRREVLHTQGEAGLKRVVKKEHADGSV
                     TQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSAT
                     GPDGLELRREYDESGRLIQET"
BASE COUNT          139 a          148 c          173 g           82 t
ORIGIN      
        1 cgcggcgaac tggcggcggt gtatgaccgc agcaatacac aggtgcgcag ctttacttac
       61 gatgataaat accggggccg gatggtggcg caccgtcaca cgggccgacc ggaaatctgt
      121 taccgttacg acagcgacgg gcgggtgaca gaacagctaa acccggcagg cttaagttac
      181 acgtatcagt atgagaaaga ccacatcacc atcaccgaca gcctgaaccg ccgtgaagtg
      241 ctgcacacgc agggcgaagc cgggctgaag cgggtggtga aaaaggaaca cgcggacggc
      301 agcgtcacgc agagtcagtt tgatgcggtg ggcaggctta aggcacagac ggatgccgca
      361 ggcaggacaa cagagtacag cccggatgtg gtgacgggac tcatcacgcg catcaccacg
      421 ccggatggca gggcatcggc gttttactat aaccaccaca gccagttaac gtcagccacc
      481 gggcctgacg ggctggaatt gcgccgggaa tatgatgaat ccggccgtct gattcaggaa
      541 ac
//
LOCUS       DAZGAN020000439          541 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZGAN020000439 DAZGAN020000000
VERSION     DAZGAN020000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.446"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..145
                     /locus_tag="ACHKYO_RS27365"
                     /old_locus_tag="pgaptmp_005473"
     CDS             <1..145
                     /locus_tag="ACHKYO_RS27365"
                     /old_locus_tag="pgaptmp_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432089.1"
                     /translation="VSAKFARYVPVEPYTTQRDGELVTVTIKTAAATGTLNVLLRYTV
                     VGY"
     gene            195..>541
                     /locus_tag="ACHKYO_RS27370"
                     /old_locus_tag="pgaptmp_005474"
     CDS             195..>541
                     /locus_tag="ACHKYO_RS27370"
                     /old_locus_tag="pgaptmp_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432090.1"
                     /translation="MSEKIAVVYIGPKTVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEA"
BASE COUNT          129 a          123 c          162 g          127 t
ORIGIN      
        1 tgtttctgca aaatttgcca gatatgtgcc agtggagccg tacaccacac agcgtgacgg
       61 ggagctggtt actgtcacca ttaagactgc cgctgcaacc ggcaccctga atgttctgct
      121 gcgttatacc gtggtgggat actgattaaa accttccggc ccgcgtcatg cgggcttttt
      181 atccggggaa ttatatgagt gagaaaattg ccgttgtcta tatcggccca aaaaccgtga
      241 aaaaggacac cattaccgga agccgcacat tgttcccacg tcttgagccg gtgcatgttg
      301 acagtgcgat ggcctggcaa ctgctggggt ttccggatgt ctgggttcgt catgaagagc
      361 ttgatgatgt tctgaaaaag caacaacaga atgagcagtt gcggcaggca cagcaggcgc
      421 aggaaagagt gcttgctgcg cgggcagaag cggagaacag ttttgttgtt tctgttaacg
      481 ggcaggaggt ggatttaagt aagctcacct cagcacggct ggcgacgctg tgtgaggcag
      541 a
//
LOCUS       DAZGAN020000440          537 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZGAN020000440 DAZGAN020000000
VERSION     DAZGAN020000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.447"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..521
                     /locus_tag="ACHKYO_RS27375"
                     /old_locus_tag="pgaptmp_005475"
     CDS             <1..521
                     /locus_tag="ACHKYO_RS27375"
                     /old_locus_tag="pgaptmp_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4432091.1"
                     /translation="VHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTK
                     PLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALR
                     AVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVS
                     ELLPWRIALPAE"
BASE COUNT          133 a          139 c          161 g          104 t
ORIGIN      
        1 atgtgcacgt ccgcatcccg tcagcactga cggaagaagc cctggagcag atcggtcagt
       61 tgtacgccat agaggcggat ataaggggaa tgccggcaga gcagcggctt gctgaacgtc
      121 agcgaaaaac gaaaccgttg ttgaaatccc tggaaagctg gttgcgtgaa aagatgaaga
      181 ccctgtcgcg acactcagag ttggcgaagg cgttcgcgta cgcacttaac cagtggccgg
      241 cactgacgta ctatgcgaac gatggctggg tggaaatcga caacaacatc gctgaaaatg
      301 ccctgcgggc ggtcagtctg ggtcgtaaaa acttcctgtt cttcggctct gaccatggtg
      361 gtgagcgggg agcgctactg tacagcctga tcgggacgtg caaactgaat gacgtggatc
      421 cagaaagcta ccttcgccat gtgcttggcg tcatagcaga ctggccggtc aaccgggtca
      481 gcgaactgct tccgtggcgc atagcactgc cagctgaata acacatcccc gtcaata
//
LOCUS       DAZGAN020000441          534 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZGAN020000441 DAZGAN020000000
VERSION     DAZGAN020000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.448"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(167..>534)
                     /locus_tag="ACHKYO_RS27380"
                     /old_locus_tag="pgaptmp_005476"
     CDS             complement(167..>534)
                     /locus_tag="ACHKYO_RS27380"
                     /old_locus_tag="pgaptmp_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4432092.1"
                     /translation="LRYNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLS
                     PVKRRLLEEVVYVITETVRKTAMQIRWRGMYQRLLKVDPLKCILCGCQMRFTGLKRGY
                     RLTELVLMHEPLAQQRVCG"
BASE COUNT          132 a          175 c           98 g          129 t
ORIGIN      
        1 aaaaagatta aaaaaacacc caaaaacgga tgtttattca acactgcctg cgtgccatga
       61 gtgcaccgcc gtcaattaaa tggatcttat cgattgatac tgtatatcac ctcaggtttt
      121 ttactccatt cccctgaaaa tggacgcaac ttccccgatg cggctctcag ccgcacaccc
      181 gctgttgcgc cagtggctca tgcatcagga ccagctctgt cagacggtag ccccgcttca
      241 gccccgtaaa acgcatctga catccgcaca ggatgcactt cagcgggtca accttcagta
      301 accgctgata catccctctc cacctgattt gcatcgccgt ctttctcacc gtctccgtta
      361 tgacgtacac cacttcttcc agtaaccggc gcttcaccgg actcaggaaa ccgtagtacc
      421 tcaccatacg gaacccctta tccgccacat gccaggagaa cctttccata aactcatcac
      481 cactcatcac caggtattct tcccgttttg tccggtgact gttgtaacgc agac
//
LOCUS       DAZGAN020000442          529 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZGAN020000442 DAZGAN020000000
VERSION     DAZGAN020000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.449"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..473)
                     /locus_tag="ACHKYO_RS27385"
                     /old_locus_tag="pgaptmp_005477"
     CDS             complement(<1..473)
                     /locus_tag="ACHKYO_RS27385"
                     /old_locus_tag="pgaptmp_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HGU4432093.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHFFSSKLFKYVMLFFSFCY"
BASE COUNT          179 a          101 c           79 g          170 t
ORIGIN      
        1 tgatagcaaa aagaaaaaaa aagcattaca tacttaaaca gcttactgct aaagaaatgt
       61 tgttttgtag aaataatgtt attttcaata atgcctggca atgaatactg ccaaacttca
      121 tttttttctt ttacagcaaa tttgagacct aaaacttcta tttgctgatt caatgttaac
      181 gttctatctt caggttcttg tgctgttaat tctctgaatg ttactaccag atcagggtcg
      241 gtagtaatta ttatctcaaa actacctgta agctcgccat aaggaacgac tattgtgttt
      301 tctggtaact cagaaaaagc atcacttctt cctaacgaaa catcattacc aatgataaca
      361 agtagacgcc cattaagtat ttcatactcg catccattca atggtccgct caacaaacga
      421 atggcataag attccaataa gtcttgtgat aagaatttat cattattttc catgaatacc
      481 acccgctaat tccataagct ctagtttaga gtagtgtaac aagacggaa
//
LOCUS       DAZGAN020000443          527 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZGAN020000443 DAZGAN020000000
VERSION     DAZGAN020000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.450"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..123
                     /locus_tag="ACHKYO_RS27390"
                     /old_locus_tag="pgaptmp_005478"
     CDS             <1..123
                     /locus_tag="ACHKYO_RS27390"
                     /old_locus_tag="pgaptmp_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432094.1"
                     /translation="TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            125..406
                     /locus_tag="ACHKYO_RS27395"
                     /old_locus_tag="pgaptmp_005479"
     CDS             125..406
                     /locus_tag="ACHKYO_RS27395"
                     /old_locus_tag="pgaptmp_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HGU4432095.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            403..>527
                     /locus_tag="ACHKYO_RS27400"
                     /old_locus_tag="pgaptmp_005480"
     CDS             403..>527
                     /locus_tag="ACHKYO_RS27400"
                     /old_locus_tag="pgaptmp_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432096.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKG"
BASE COUNT          138 a          112 c          169 g          108 t
ORIGIN      
        1 acgccgccgg taggcaacgt gcagggaacc ggtgaaattg aaatcaccat acagaccaac
       61 ggcctgcgcc gccgtgtgaa ggccacccgc atcagcggct gcacggttta ctgggaggtg
      121 tgaggtgaaa aaaaatctca ttgcatgggc gtggtcgagt ggtcttattg agtttggtta
      181 cgtcctgccg gaaggtgcat tgccgatagt tgccggaaag cctgccacgg tacggcatgt
      241 gattgaggtt atggcgcgtc atggacgtga tgaacaggag cagttactgg ttccggggat
      301 accggaagcg gtgacggagg aagaagcctt taatgccatg attcggttct gccgtgaggt
      361 cagacgccgg gtcagttatc caaacagaac gaggaccaga ggatgagtaa agtcgtacgc
      421 attattttcg aatacaagga gcacgttatc cataaaaacg ctgatggaac agtgcgcatg
      481 ggggtaagtc tggacatacg ttcaaccggg ataaagcaga aaggtga
//
LOCUS       DAZGAN020000444          524 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZGAN020000444 DAZGAN020000000
VERSION     DAZGAN020000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.451"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..319
                     /gene="elaD"
                     /locus_tag="ACHKYO_RS27405"
                     /old_locus_tag="pgaptmp_005481"
     CDS             <1..319
                     /gene="elaD"
                     /locus_tag="ACHKYO_RS27405"
                     /old_locus_tag="pgaptmp_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase ElaD"
                     /protein_id="HGU4432097.1"
                     /translation="IFLHADLQQYLSQSCGAFVCMAAQEVIEQRESNSDSAPYTLLKN
                     YADRFKKYSAEEQYEIDFQHRLINRNCYLDKYGDANINHYYKNLEIKHTQPKNRASGK
                     RVS"
BASE COUNT          175 a          100 c          100 g          149 t
ORIGIN      
        1 aatattttta catgccgatc ttcagcaata cttaagccaa agttgcggtg cgtttgtgtg
       61 catggcagcc caggaagtga ttgaacaaag ggaaagcaat tctgacagcg ccccctatac
      121 gttattaaaa aactatgctg acagatttaa aaaatattca gcagaagagc agtacgaaat
      181 tgattttcaa catcgactga taaacagaaa ttgttattta gataaatatg gcgatgctaa
      241 tatcaatcat tattataaaa acttagaaat aaaacacaca caacccaaaa atagagcgtc
      301 aggaaaaaga gtaagttaac atcattgtta attaattcga ccagtcagta aatatgctga
      361 ctggtaggac atagtttgct aaaaagtcat cactgactga tgccagtcac accatccgcc
      421 agttcaatct tcgggtattt ccagcgtatc tcggcacgcg attgctggtc tgttgatgta
      481 gtggattgtt gttgctgact tttcttacca tcagatttct gccc
//
LOCUS       DAZGAN020000445          508 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZGAN020000445 DAZGAN020000000
VERSION     DAZGAN020000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.452"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..101
                     /locus_tag="ACHKYO_RS27410"
                     /old_locus_tag="pgaptmp_005482"
     CDS             <1..101
                     /locus_tag="ACHKYO_RS27410"
                     /old_locus_tag="pgaptmp_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4432098.1"
                     /translation="YPAQEHLLLQLCMRVTKKLTDNINSSLKGGYK"
     gene            112..480
                     /locus_tag="ACHKYO_RS27415"
                     /old_locus_tag="pgaptmp_005483"
     CDS             112..480
                     /locus_tag="ACHKYO_RS27415"
                     /old_locus_tag="pgaptmp_005483"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGU4432099.1"
                     /translation="MVLALLSSADNFFLSPTELSEKLDISRTNITRVFDSLEKFGFIR
                     RMESKEDRRSKNIYLTPDGDLLLQKITRIYGRYLKKYGATLQMIKSRYLSNQQKDVIS
                     KYFSVISHVIMHINPEFLFL"
BASE COUNT          172 a           78 c           88 g          170 t
ORIGIN      
        1 catatccagc tcaggagcat ctccttttac aactttgtat gcgtgttacc aaaaaactta
       61 ctgataatat taattcatcc ctgaaggggg ggtataaatg atacaaagct tatggttctg
      121 gccctcttat ccagtgctga taattttttc ttatctccaa ctgaactcag tgaaaaatta
      181 gatatttcca gaacaaatat caccagagtt tttgattctc tagaaaaatt tggttttatt
      241 aggcgtatgg aaagtaagga agatcgacgt agtaagaata tttacctgac tccagatggt
      301 gatttgttat tacagaagat tacaaggata tatggacgct atctaaaaaa atatggagca
      361 accttacaga tgataaagtc aagatattta agtaatcagc aaaaagatgt tatctcaaaa
      421 tatttctcag taatatcaca tgtaatcatg catataaatc ctgagttctt atttttataa
      481 tttattattc tgcaattgtg tgtggggg
//
LOCUS       DAZGAN020000446          506 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZGAN020000446 DAZGAN020000000
VERSION     DAZGAN020000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.453"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..450
                     /locus_tag="ACHKYO_RS27420"
                     /old_locus_tag="pgaptmp_005484"
     CDS             <1..450
                     /locus_tag="ACHKYO_RS27420"
                     /old_locus_tag="pgaptmp_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4432100.1"
                     /translation="NCTINGTLKAENIIGDLVKCAGVAFPVDGSYLANGTRTLTVYDD
                     HSFDRQIIIPPIIYVGSKQESRTSNDIWTECFLHVDQNGRRIYSGRSVTEPGIFSGII
                     DMPAGHGHITLSFTVSSRRQNGSFGSSRISNLQAIVVKKNSAGISIR"
BASE COUNT          134 a          112 c          146 g          114 t
ORIGIN      
        1 aactgtacga taaatggcac gctgaaagcg gagaacatta ttggtgatct tgtgaaatgt
       61 gcaggggtgg cttttccggt ggatggtagt taccttgcga acggtacacg aacgctgacg
      121 gtgtatgacg atcacagctt tgaccggcag attataatcc cgccgataat ctatgtcggg
      181 tcaaaacagg aatcccgcac cagtaatgac atctggacag agtgcttcct gcatgttgat
      241 cagaacggac gccggattta ttcaggcagg tcagtgacag agccgggaat tttcagcggg
      301 atcatcgata tgccagccgg tcatggtcat atcaccctga gttttaccgt aagttcacga
      361 cgtcagaacg ggagttttgg cagttcacgt atcagtaacc ttcaggcgat agtggtgaag
      421 aaaaacagcg cggggatcag tatccgctga acatcgcgcc ccgggattgc cggaaggaga
      481 caaaaaccgt acagtatgcg cgggtg
//
LOCUS       DAZGAN020000447          497 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZGAN020000447 DAZGAN020000000
VERSION     DAZGAN020000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.454"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>497)
                     /locus_tag="ACHKYO_RS27425"
                     /old_locus_tag="pgaptmp_005485"
     CDS             complement(<1..>497)
                     /locus_tag="ACHKYO_RS27425"
                     /old_locus_tag="pgaptmp_005485"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGU4432101.1"
                     /translation="HQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRYSV
                     AAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEDPALAAKIYTKPFPLVDI
                     TVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIVNA
                     GDSVSP"
BASE COUNT          115 a          133 c          127 g          122 t
ORIGIN      
        1 ggtgatacag agtcccctgc gttaacgatg taatgaatca gaacacgcac ctgctcgtct
       61 gaaatttcca ccatgacctg cgggagtttg tccagcagct ccatcatgtc acgatggcgg
      121 atatgcttca tcagcagcgt cagtgcggcc atccggcgat ggttcatgat ttcattgtca
      181 tcaacgacag tgatatcaac cagcggaaac ggctttgtgt atattttagc cgcaagtgcc
      241 ggatcctcaa aacagtccag ccagttcatg ctgtacgggt acgggctgcg ctcaccgtga
      301 tagaacagca ccggaatgac cagtggcaac gttttgtgct gctccagatg tctctgcatc
      361 gccgccacac tgtaacgtaa catgcggaaa ggcatgtaca gattttcggt gctctggtgc
      421 tcgatgagcg tataaacata gccgtcttcc cccccggtgg ttttcacact ccagaggata
      481 tcgctggcgt actgatg
//
LOCUS       DAZGAN020000448          493 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZGAN020000448 DAZGAN020000000
VERSION     DAZGAN020000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.455"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..171
                     /locus_tag="ACHKYO_RS27430"
                     /old_locus_tag="pgaptmp_005486"
     CDS             <1..171
                     /locus_tag="ACHKYO_RS27430"
                     /old_locus_tag="pgaptmp_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4432102.1"
                     /translation="AVMQAIAEKPGALRVLTHTLNQAWLTASGEGAALTEKHINAAFK
                     EVYTNPELLSQV"
     gene            174..395
                     /locus_tag="ACHKYO_RS27435"
                     /old_locus_tag="pgaptmp_005487"
     CDS             174..395
                     /locus_tag="ACHKYO_RS27435"
                     /old_locus_tag="pgaptmp_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432103.1"
                     /translation="MAVFYIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATEVYSGICRSVGAKQVS"
     gene            408..>493
                     /locus_tag="ACHKYO_RS27440"
                     /old_locus_tag="pgaptmp_005488"
     CDS             408..>493
                     /locus_tag="ACHKYO_RS27440"
                     /old_locus_tag="pgaptmp_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432104.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAV"
BASE COUNT          131 a          105 c          126 g          131 t
ORIGIN      
        1 gccgtcatgc aggctatcgc tgaaaagccg ggagcgttac gcgttctgac acatacgctt
       61 aaccaggcgt ggctcaccgc cagcggtgaa ggtgcggcgc tgacagaaaa acatattaat
      121 gcggccttta aagaggttta taccaaccct gaattactct cacaggtgtg attatggcgg
      181 tattttatat tcctgatatt tacggacgct tttacctggt taatttcgat aacgtgaagg
      241 tgatttcact ggccgaaaat aaagaatgtg gcgatttact ttttgaattt aatgaccgca
      301 cacgaatggt gatatctgcc ggacttgatc gcgaaggtgc gacagaagtt tacagcggaa
      361 tatgccgttc tgttggtgcg aaacaagtca gctaaatgag gtgttatatg aatatgcaat
      421 cctgcggtaa caaaatgaat ttattcgact ccctgaacag cgcgcgccgt ctgaccgaac
      481 ttgccggtgc ggt
//
LOCUS       DAZGAN020000449          492 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZGAN020000449 DAZGAN020000000
VERSION     DAZGAN020000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.456"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..302)
                     /locus_tag="ACHKYO_RS27445"
                     /old_locus_tag="pgaptmp_005489"
     CDS             complement(<1..302)
                     /locus_tag="ACHKYO_RS27445"
                     /old_locus_tag="pgaptmp_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4432105.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDT"
BASE COUNT          130 a          133 c          113 g          116 t
ORIGIN      
        1 gtatccggta atggcagtcc ctcaccgtat gacataccat tggactgacc agcaagcgcg
       61 atcacatagt aatattctgg cgcaacagaa ggcgctgagg tcgtcggacg gttgcctggc
      121 tcctctggtg atgagatgct cccctcactc acaactggct ggatgaactc cgcaccataa
      181 ccagctgtcg aaatcagcgc actaccataa ggctgccacc cttccttcag tttttgagtt
      241 attcgttccg caaggtctga cggcgacgcc gccctgacca cgtcatagtg tttaaatgcc
      301 atgaatcctc ccggccggga taatgtactg aataagataa ggagtgagcg gaagtcccga
      361 aacacaaggg aaaaagacaa ccacccgcaa aaaagaaata cactcctcac agttgcgcag
      421 ggtgattact gtaaggtatt attcgcagcg ttaattaatt gttcatattg cttttcttct
      481 tgccaaccgc tc
//
LOCUS       DAZGAN020000450          492 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZGAN020000450 DAZGAN020000000
VERSION     DAZGAN020000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.457"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>492
                     /locus_tag="ACHKYO_RS27450"
                     /old_locus_tag="pgaptmp_005490"
     CDS             <1..>492
                     /locus_tag="ACHKYO_RS27450"
                     /old_locus_tag="pgaptmp_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU4432106.1"
                     /translation="ASENPDEAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQ
                     PGTLNDFLGAMTEDDARPEALRRFELMVEEVARNASAVAQNTAAAKKSASDASTSARE
                     AATHAADAADSARAASTSAGQAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAA
                     TSA"
BASE COUNT          112 a          142 c          155 g           83 t
ORIGIN      
        1 tggcctcaga aaatccggat gaagccgggc gttacagcat ggacgttgag tacggtcagt
       61 acagcgttat tctgttggtg gaaggattcc cgccgtcaca tgccgggacc atcaccgtgt
      121 atgaagattc tcaacccggt acgctgaatg attttctcgg tgccatgacg gaggatgatg
      181 cccgtccgga ggcactgcgc cgttttgaac tgatggtgga agaggtggcg cgtaacgcgt
      241 ccgcggtggc acagaacacg gcagccgcga agaagtcagc cagtgatgcc agcacatcag
      301 cccgtgaggc ggcaacccat gcggctgatg ctgcggactc agcacgcgca gccagcacgt
      361 cagccggaca ggccgcgtcg tcggctcagt cagcgtcttc cagcgcagga acggcatcaa
      421 caaaggccac tgaagcatca aaaagtgctg ccgctgcaga gtcctcaaaa agcgcggcgg
      481 ccaccagtgc cg
//
LOCUS       DAZGAN020000451          486 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZGAN020000451 DAZGAN020000000
VERSION     DAZGAN020000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.458"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>486)
                     /locus_tag="ACHKYO_RS27455"
                     /old_locus_tag="pgaptmp_005491"
     CDS             complement(<1..>486)
                     /locus_tag="ACHKYO_RS27455"
                     /old_locus_tag="pgaptmp_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4432107.1"
                     /translation="PEPDRVWCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHAD
                     TALISSALRMAYETRGQPRDVVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDN
                     SPMERFFRSLKTEWVPTNGYAGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENR
                     Y"
BASE COUNT          102 a          139 c          118 g          127 t
ORIGIN      
        1 ggtatctgtt ctctgactct tccggcgtca gcccaccgtt ataatggtga ggtctgacgc
       61 tgttgtagta gttcaatatg taatcattaa tttgctgccg ggcctcgtcc ttgcctgcgt
      121 aaccattcgt tggcacccat tctgttttca gactgcggaa gaagcgttcc atggggctgt
      181 tatcccagca gtttccccgc cgactgacgc tttgatttat tctgcaacgc cagagaagtt
      241 gttgatattt aaggcctgta tactggcttc cctggtcgct atggaacacg acatcccggg
      301 gttggccacg cgtctcatag gccatccgca gggcactgct tatcagtgcg gtatcggcat
      361 gcgctgacag actccagccg ataaccctgc gggcaaaaag atccataaca accgccagat
      421 agcaccagcg atttcctgcc cagagatacg taatatctcc acaccatacc cggtctggct
      481 ccggta
//
LOCUS       DAZGAN020000452          479 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZGAN020000452 DAZGAN020000000
VERSION     DAZGAN020000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.459"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..479
                     /locus_tag="ACHKYO_RS27460"
                     /old_locus_tag="pgaptmp_005492"
                     /pseudo
     CDS             1..479
                     /locus_tag="ACHKYO_RS27460"
                     /old_locus_tag="pgaptmp_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          114 a          138 c          138 g           89 t
ORIGIN      
        1 gcaaaatcag cagacgtctt ctgaacgtga actggagatg tctaccgaga ttgcacgtct
       61 caaacgccag ctggcagaac gggatgaaga gctggctatc ctccaaaagg ccgcgacata
      121 cttcgcgaag cgcctgaaat gaagtatgtc tttattgaaa aacatcaggc tgagttcagc
      181 atcaaagcaa tgtgccgcgt gctccgggtg gcccgcagcg gctggtatac gtggtgtcag
      241 cggcggacaa ggataagcac gcgtcagcag ttccgccaac actgcgacag cgttgtcctc
      301 gcggctttta cccggtcaaa acagcgttac ggtgccccac gcctgacgga tgaactgcgt
      361 gctcagggtt acccctttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcagggactg
      421 agggcaaagg cctcccggaa gttcagcccg gtcagctacc gcgcacacgg cctgcctgt
//
LOCUS       DAZGAN020000453          477 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZGAN020000453 DAZGAN020000000
VERSION     DAZGAN020000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.460"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..454)
                     /locus_tag="ACHKYO_RS27465"
                     /old_locus_tag="pgaptmp_005493"
     CDS             complement(<1..454)
                     /locus_tag="ACHKYO_RS27465"
                     /old_locus_tag="pgaptmp_005493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432108.1"
                     /translation="MARLSESLADGFYANSDYIAWASPLVFDGDGKVLNIGFENDPVF
                     RVLGDGAGFWEAVKAMGPGLTTPDREYDSSPDNIVLYTDLYGSFLWNIIPFSVLALPL
                     GSWSAHFAGSKTDAFERILGAQFYDYMSPDSTVIVDEQSSWTSWYSWVH"
BASE COUNT          146 a          133 c           93 g          105 t
ORIGIN      
        1 catgaaccca tgaataccaa gatgtccagc tactttgctc atccacaatg actgtactgt
       61 ccggtgacat gtagtcgtaa aactgagccc ctaaaattct ttcaaaggcg tcagtttttg
      121 aacccgcaaa atgcgcactc cagcttccca gcgggagtgc aagcacagaa aaggggataa
      181 tattccagag gaatgaacca taaaggtctg tgtataaaac aatgttatca ggagaagagt
      241 catattcacg atccggggtt gtcaagcccg gccccattgc tttaactgcc tcccagaacc
      301 cggctccatc tcccagcaca cgaaataccg gatcgttttc aaaaccgata tttaatacct
      361 tcccatcccc gtcaaatacc agcggggaag cccaggctat atagtcagaa ttagcataaa
      421 aaccatcagc cagagactca ctgagtctgg ccataatgtt agtcaaccca cccccca
//
LOCUS       DAZGAN020000454          476 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZGAN020000454 DAZGAN020000000
VERSION     DAZGAN020000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.461"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>476
                     /locus_tag="ACHKYO_RS27470"
                     /old_locus_tag="pgaptmp_005494"
     rRNA            <1..>476
                     /locus_tag="ACHKYO_RS27470"
                     /old_locus_tag="pgaptmp_005494"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZGAN020000455          475 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZGAN020000455 DAZGAN020000000
VERSION     DAZGAN020000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.462"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(81..>475)
                     /locus_tag="ACHKYO_RS27475"
                     /old_locus_tag="pgaptmp_005495"
     CDS             complement(81..>475)
                     /locus_tag="ACHKYO_RS27475"
                     /old_locus_tag="pgaptmp_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HGU4432109.1"
                     /translation="GLVALSGRTDRIDAAVGSVDRRVTKNTQAIQSNTRQLQEHNARL
                     NSQQRQIRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYR
                     FNEQTAAKAGVAASDGDVSYNVGVNFEF"
BASE COUNT          110 a          146 c          104 g          115 t
ORIGIN      
        1 cacctgtttc atgctcacat gagaaaaaac ggcaggcaaa actccacgcc tgccgtcatg
       61 cttcatgtca ctgtcagcaa ttaaaactca aagttcacac ctacgttgta ggacacatcg
      121 ccatcactcg ctgccacacc tgctttcgct gctgtctgct cattgaaacg gtaaccaaca
      181 cccacggcaa cagcctgttt atcactgtac ccccccaggg cagcagtggc gttgaatttc
      241 cccacactgt acggctggaa aagccccgcc agcgctgcac tctgtgctgc tgcacgcttc
      301 atctcttcgt gattctcacg aatttgacgc tgctggctgt tcaggcgtgc attatgttcc
      361 tgcaactggc gagtattgga ctgaatagcc tgtgtatttt tagtaacccg gcggtcaacg
      421 ctaccaactg cagcatcaat acggtctgtt ctgccggaca gagcaacgag accgt
//
LOCUS       DAZGAN020000456          474 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZGAN020000456 DAZGAN020000000
VERSION     DAZGAN020000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.463"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>474
                     /locus_tag="ACHKYO_RS27480"
                     /old_locus_tag="pgaptmp_005496"
     CDS             <1..>474
                     /locus_tag="ACHKYO_RS27480"
                     /old_locus_tag="pgaptmp_005496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432110.1"
                     /translation="LALDIPVKDSDYLIRINDSNNELASVIDTKTNKEVLGAINTTLG
                     SVMTWNEQDAKKVEEFIKTLPPEQQQVGLQMAMQQILFPVDSPSLKLDKLNSGTLTKA
                     DIDAVVSLKTDVSKAISIETAPAYVAAVRSGGNSDAAVAAAKDGPGVLQEYNRIVT"
BASE COUNT          153 a           89 c           97 g          135 t
ORIGIN      
        1 ctggctttag atatcccagt taaagacagt gattatttaa ttagaataaa tgattctaac
       61 aatgaattgg catctgttat tgatacaaaa acaaataagg aagttttagg cgctataaac
      121 actacactag gtagtgtcat gacatggaat gaacaggatg ctaaaaaagt cgaagaattt
      181 attaaaacat tgccgccaga gcaacaacaa gttggtctcc aaatggcaat gcaacaaatt
      241 ttgttcccag ttgattctcc tagtttaaag cttgataagt tgaattcagg aacattaacc
      301 aaggctgata ttgatgctgt tgtatcctta aaaacggatg tttcaaaggc tatttctatc
      361 gaaacagcgc cagcctatgt tgccgctgtc cgttctggcg gtaactcaga tgccgctgtc
      421 gcagcagcta aagacggtcc tggtgttctg caggaataca atcgcattgt tacc
//
LOCUS       DAZGAN020000457          470 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZGAN020000457 DAZGAN020000000
VERSION     DAZGAN020000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.464"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>470
                     /locus_tag="ACHKYO_RS27485"
                     /old_locus_tag="pgaptmp_005497"
     CDS             <1..>470
                     /locus_tag="ACHKYO_RS27485"
                     /old_locus_tag="pgaptmp_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4432111.1"
                     /translation="LLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAAN
                     LDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDRHGRLTKKTDLI
                     PEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWR"
BASE COUNT          110 a          161 c          128 g           71 t
ORIGIN      
        1 ctgctgtctg accgcgatta cacctggaac gacaacggcg aactcatccg catcagcagc
       61 ccgcgccaga cccggagtta cagctacagc accaccggca ggctgaccgg cgttcacacc
      121 accgcagcga accttgatat ccgcatcccg tatgccacag acccggcagg taaccgcctg
      181 cccgacccgg agctgcaccc ggacagcacc ctcagcatgt ggccggataa ccgtatcgcc
      241 cgtgacgcgc actatcttta ccggtatgac cgtcacggca ggctgacaaa gaaaaccgac
      301 ctcatcccgg aaggggttat ccgcacggat gatgagcgga ctcaccggta ccattacgac
      361 agtcagcacc ggctggtgca ctacacgcgg acacaatatg cagagccgct ggtcgaaagt
      421 cgctatcttt acgacccgct gggccgcagg gtggcaaaac gggtatggcg
//
LOCUS       DAZGAN020000458          468 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZGAN020000458 DAZGAN020000000
VERSION     DAZGAN020000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.465"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..317)
                     /locus_tag="ACHKYO_RS27490"
                     /old_locus_tag="pgaptmp_005498"
     CDS             complement(<1..317)
                     /locus_tag="ACHKYO_RS27490"
                     /old_locus_tag="pgaptmp_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HGU4432112.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPE"
     gene            complement(317..>468)
                     /locus_tag="ACHKYO_RS27495"
                     /old_locus_tag="pgaptmp_005499"
     CDS             complement(317..>468)
                     /locus_tag="ACHKYO_RS27495"
                     /old_locus_tag="pgaptmp_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432113.1"
                     /translation="QTGVSWSDPLRAQYFSVQFSEGIRRAYRHTLATSPYSSYRNPVR
                     RQRFY"
BASE COUNT          121 a          133 c          110 g          104 t
ORIGIN      
        1 cattcaggaa tgcccttcat tgtgggccac tcaggatact gcgcatccag cacctcccgg
       61 gataatggtc tgactgcact accgtcactg aggcatatca cgtcaagaag ttgtattaca
      121 ccatcgggca aaacctgacg ggcgccagga acacaactga ttgtttccag gcttgcgcca
      181 gcatccggtc tcgccagaat cactgccctc acagcatcat tgtaataatc gcacaattcc
      241 tgcaggggcc agcgaaccat catcgggtca accagttgtg tattcacacg tccgatgatt
      301 tctgtaatcg tcgtcatcag taaaacctct gcctgcgtac agggttgcgg tatgaagagt
      361 acgggcttgt cgccagtgta tgacgatatg cccgacggat cccctcagaa aactgcacag
      421 aaaaatactg tgcgcgtaac ggatctgacc atgaaacacc agtctgca
//
LOCUS       DAZGAN020000459          467 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZGAN020000459 DAZGAN020000000
VERSION     DAZGAN020000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.466"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>467
                     /locus_tag="ACHKYO_RS27500"
                     /old_locus_tag="pgaptmp_005500"
     CDS             <1..>467
                     /locus_tag="ACHKYO_RS27500"
                     /old_locus_tag="pgaptmp_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HGU4432114.1"
                     /translation="LSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEG"
BASE COUNT          126 a          113 c          113 g          115 t
ORIGIN      
        1 ctttccactg ccagcatcga tgattttacc cgttattcta aagatcttgc agatgaaggc
       61 acccgctgct ttatcgatgc cgataatatg cgtgccgtca gtgtgcttaa cctgggtact
      121 attgatgagc caggtcacgc agataacact gccactctca aactgaaaaa gacagcaccg
      181 ttctctgctc tgttgtctgt taacggcgag cgtaactccc agaagtcact ggcagaatgg
      241 atcgaagact gggccgacta ccttgtgggc tttgatgcta atggtgacgc cattcaagca
      301 acaaaagcgg ctgcggcagt ccgtaaaatc acgattgaag caaaccagac cgctgatttt
      361 gaagataatg acttcagcgg caaacgctcc ctgatggagt ctgtcgaagc gaagaccaaa
      421 gacattatgc cagtggcatt tgaatttaaa tgcgttccgt ttgaagg
//
LOCUS       DAZGAN020000460          458 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZGAN020000460 DAZGAN020000000
VERSION     DAZGAN020000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.467"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(164..382)
                     /gene="ymgF"
                     /locus_tag="ACHKYO_RS27505"
                     /old_locus_tag="pgaptmp_005501"
     CDS             complement(164..382)
                     /gene="ymgF"
                     /locus_tag="ACHKYO_RS27505"
                     /old_locus_tag="pgaptmp_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HGU4432115.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
BASE COUNT          164 a           93 c           82 g          119 t
ORIGIN      
        1 tcattgatag ttgcaataaa aattatttca atgacatata aaaacaactg tgataacacc
       61 agggttgaca actatttgcg caataattcc tttacttcaa tgatccaggc attcactgcc
      121 agcaacccat tcagccgcat actgacagtg agaaactgaa agattattgc tgatccctgt
      181 ggctgacgag tctttccctt aatcgattaa ccagattgat cgccagccaa acaagggcat
      241 taagcaaatt tatcgtcaca agcacttctc ccagcccggt actgggtttt gcaataagga
      301 tgaccaggag ggtgatcagc ataaatgcaa tggaaaagat gatatagaga gtgaaataat
      361 ccagattatt actattgttc atgctactaa tggcaatcct gtcaaaactt tcaaacaatt
      421 atagcaaaca aaataatgaa caatagtaac aagaggca
//
LOCUS       DAZGAN020000461          454 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZGAN020000461 DAZGAN020000000
VERSION     DAZGAN020000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.468"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..317)
                     /locus_tag="ACHKYO_RS27510"
                     /old_locus_tag="pgaptmp_005502"
     CDS             complement(<1..317)
                     /locus_tag="ACHKYO_RS27510"
                     /old_locus_tag="pgaptmp_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HGU4432116.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLAELCDYYNDAVRAVILARP
                     DAGASLETLSCVPGARQTLPDGAIQLLDVICLSDGSAIKPVSREVLDAQYPDWHMLRG
                     KPE"
     gene            complement(317..>454)
                     /locus_tag="ACHKYO_RS27515"
                     /old_locus_tag="pgaptmp_005503"
     CDS             complement(317..>454)
                     /locus_tag="ACHKYO_RS27515"
                     /old_locus_tag="pgaptmp_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432117.1"
                     /translation="SWSDPSRAQYFSVQFSEGIRRAYRHTLATSPYSSYRNPVRRQRF
                     Y"
BASE COUNT          108 a          131 c          113 g          102 t
ORIGIN      
        1 cattccggtt ttcccctcag catgtgccag tcgggatact gtgcatcaag cacctcccgg
       61 gatactggtt taatcgcact gccatcgctg aggcatatca cgtcaagaag ctgtattgcg
      121 ccatcaggta aagtctggcg tgcgccaggg acacaactga gcgtttccag gcttgcgcct
      181 gcatccggcc ttgccagaat caccgcccgc acagcatcat tgtaataatc acacagctca
      241 gccagaggcc agcgcaccat catcgggtca accagttgtg tattcacacg tccgatgatt
      301 tctgtaatcg tcgtcatcag taaaacctct gcctgcgtac agggttgcgg tatgaagagt
      361 acgggcttgt cgccagtgta tgacgatatg cccgacggat cccctcagaa aactgcacag
      421 aaaaatactg tgcgcgtgac ggatctgacc atga
//
LOCUS       DAZGAN020000462          453 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZGAN020000462 DAZGAN020000000
VERSION     DAZGAN020000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.469"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..453
                     /locus_tag="ACHKYO_RS27520"
                     /old_locus_tag="pgaptmp_005504"
                     /pseudo
     CDS             1..453
                     /locus_tag="ACHKYO_RS27520"
                     /old_locus_tag="pgaptmp_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          129 a          120 c          116 g           88 t
ORIGIN      
        1 aaaacaggtg acttaatgaa caagaaaacc aaacgaacct tcacccctga gttcaggctg
       61 gaatgtgcac agctgattgt tgataagggc tactcatatc gacaggccag tgaagcgatg
      121 aatgtcggtt caaccacgct tgagagctgg gtacgccagc tcaggcgaga gcgccagggt
      181 attacgccct ctgccacacc cattactcca gaccagcaac gtatccgcga gctggaaaag
      241 caagttcgcc gtctggagga acaaaatacg atattaaaaa aggctaccgc gctcttaatg
      301 tccgactcgc tgaacggttc acgatagccg ccagactaag tgacagccac acggttgtca
      361 gcctgtgttc tgctctggaa atacaccgca gcagttaccg gtactggcga aaacgacgcg
      421 atacggttaa tccggcacga gtcaggttgt gca
//
LOCUS       DAZGAN020000463          444 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZGAN020000463 DAZGAN020000000
VERSION     DAZGAN020000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.470"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            56..>444
                     /locus_tag="ACHKYO_RS27525"
                     /old_locus_tag="pgaptmp_005505"
     CDS             56..>444
                     /locus_tag="ACHKYO_RS27525"
                     /old_locus_tag="pgaptmp_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HGU4432118.1"
                     /translation="MKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIDNGKIYI
                     GLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGVI
                     TLQKDVYSYDIAKDKWEKLMTRPPVSLA"
BASE COUNT          154 a           64 c           99 g          127 t
ORIGIN      
        1 acatgaaaat agttaccgtg tcggtgtttt gattaatttt taaaaggtgg ttacaatgaa
       61 agtgaaaaac tttatatatc ttcccttctg cttgtttatt ggaacgtcgg tagccggagc
      121 tttaccggaa attcctgaac catttaaata tggtgtagga gggatcgata atggtaaaat
      181 atatatagga ctgggaagtc tgggaaataa ctggtatatg atagatacaa accagagtga
      241 aaaaaaatgg actaagatag cacaatggcc gacagttccc agagagcagg caacagctac
      301 tattattgat ggtaagatct atgtatttgg aggaattgga aaagataaaa gtggagtcat
      361 aaccttacaa aaagatgtct attcctatga tattgctaaa gataaatggg aaaaattgat
      421 gacccgtccc cctgtatctc ttgc
//
LOCUS       DAZGAN020000464          441 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZGAN020000464 DAZGAN020000000
VERSION     DAZGAN020000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.471"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..105
                     /locus_tag="ACHKYO_RS27530"
                     /old_locus_tag="pgaptmp_005506"
     CDS             <1..105
                     /locus_tag="ACHKYO_RS27530"
                     /old_locus_tag="pgaptmp_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4432119.1"
                     /translation="QYIRWIYSKGVKLPGLEARRSADEWLCRYDLPKV"
     gene            140..>441
                     /locus_tag="ACHKYO_RS27535"
                     /old_locus_tag="pgaptmp_005507"
     CDS             140..>441
                     /locus_tag="ACHKYO_RS27535"
                     /old_locus_tag="pgaptmp_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432120.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQ"
BASE COUNT           98 a          110 c          141 g           92 t
ORIGIN      
        1 cagtatatcc gctggattta ttcaaagggg gtgaaactcc ccggccttga ggcccgccgt
       61 tcggcggatg aatggttatg tcgctacgac ttgccgaaag tctgaaccgc ttctggcgac
      121 cgctcatgat agcgctgctg tgtgcggtac tgctgttacg gggtgtcctg tggctgcggt
      181 gaataatgcc ttaccggccc tgattgccgg ggcgtgcatg gcggcgctgg ggattatcgc
      241 tgtactggtt gccgcgatat ggggaatgca ccagaagacg cagcgccttg aagacaataa
      301 tcaggtgctt gtgcgtgagc gggatgaggc gcgtcaggtg ctggcaaatc agcagcacac
      361 cctgcaactc atttcacaaa tcagtgaggc ggccacgaat gaaaaacaac agaacattca
      421 gcacagcgag gggcagcaga g
//
LOCUS       DAZGAN020000465          436 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZGAN020000465 DAZGAN020000000
VERSION     DAZGAN020000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.472"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            149..>436
                     /locus_tag="ACHKYO_RS27540"
                     /old_locus_tag="pgaptmp_005508"
     CDS             149..>436
                     /locus_tag="ACHKYO_RS27540"
                     /old_locus_tag="pgaptmp_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HGU4432121.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPL"
BASE COUNT          145 a           85 c           57 g          149 t
ORIGIN      
        1 ctattttaaa tgcctctatg cataacttta tttacaattt aaggtataaa ccctattccc
       61 tcaatgttaa gcaaatataa caatcactct tattttaaat tttacacgta atatttaccc
      121 taaatgattt tagagtacag gtgggcatat gcaagtcttt tccagtgatg tctacttcac
      181 tgtaggcact aatgcattat tagcatccca aaaagaatat tacagcgatt tagttgcgtt
      241 ggttgatctt ggacattcgt tcgttgttat tgatgagcat caacatcgaa atctcaaacc
      301 aaatacagaa cctgtaaaca tccttttgag caacaatttc atcaggatca ataagaatat
      361 tacattatct gatttaacgc atttcctggt ttcaaatctt catacacaaa atgtgtattc
      421 cacacaagaa ccactt
//
LOCUS       DAZGAN020000466          434 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZGAN020000466 DAZGAN020000000
VERSION     DAZGAN020000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.473"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>434
                     /locus_tag="ACHKYO_RS27545"
                     /old_locus_tag="pgaptmp_005509"
     CDS             <1..>434
                     /locus_tag="ACHKYO_RS27545"
                     /old_locus_tag="pgaptmp_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4432122.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQA"
BASE COUNT          100 a          117 c          125 g           92 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgccgact
      361 ttcggcgtga cctacactgg taactatgag ttcccacaac cgctgcacga tgcgctggat
      421 aaattccagg ccga
//
LOCUS       DAZGAN020000467          432 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZGAN020000467 DAZGAN020000000
VERSION     DAZGAN020000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.474"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>432
                     /locus_tag="ACHKYO_RS27550"
                     /old_locus_tag="pgaptmp_005510"
     CDS             <1..>432
                     /locus_tag="ACHKYO_RS27550"
                     /old_locus_tag="pgaptmp_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4432123.1"
                     /translation="KTATSRAAEAGQKATDATEAATRAITAAGNAEESSTRAGESEKA
                     AGADAEKARQHAEKARLAQESAGEILKRAEAATVSAEEARRMAENARGPRGPQGETGP
                     KGDVGPKGETGPVGPQGPAGPKGERGDVGAQGAVGPAGPRGE"
BASE COUNT          106 a          109 c          166 g           51 t
ORIGIN      
        1 aagacggcaa ccagcagggc ggctgaagcc ggacaaaaag cgactgatgc cactgaggcc
       61 gcgacccgtg caatcacagc agcggggaat gcagaggaaa gctcgacccg tgccggagag
      121 tctgaaaaag ccgccggagc tgatgcagaa aaagccagac agcatgctga aaaggccagg
      181 ctggcgcagg agagcgccgg agagatcctt aagcgggcag aggctgccac tgtcagtgct
      241 gaagaggcca gacgtatggc tgagaatgca cgggggcccc gggggcctca gggagaaact
      301 ggtccgaagg gggatgtcgg tcctaaaggc gaaacaggtc cagtgggccc tcaagggccc
      361 gcagggccga aaggtgagcg tggtgacgtt ggtgctcagg gggctgtagg gcccgctggt
      421 ccgcgtggtg ag
//
LOCUS       DAZGAN020000468          413 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZGAN020000468 DAZGAN020000000
VERSION     DAZGAN020000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.475"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..319
                     /locus_tag="ACHKYO_RS27555"
                     /old_locus_tag="pgaptmp_005511"
     CDS             <1..319
                     /locus_tag="ACHKYO_RS27555"
                     /old_locus_tag="pgaptmp_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGU4432124.1"
                     /translation="QMLRKKATLGCSTVILRVSAFPDVRMPDLRTGQDYATWLKILKR
                     GTRAYLLPEVLTSYRIVNGSISRNKFKKALRQWEIYRKYENLSFLYSCYCFSLYAFRA
                     VVR"
     gene            324..>413
                     /locus_tag="ACHKYO_RS27560"
                     /old_locus_tag="pgaptmp_005512"
     CDS             324..>413
                     /locus_tag="ACHKYO_RS27560"
                     /old_locus_tag="pgaptmp_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HGU4432125.1"
                     /translation="MNINLIELQQHGDERGALVSLECNKNIPFD"
BASE COUNT          140 a           54 c           83 g          136 t
ORIGIN      
        1 tcagatgtta aggaaaaaag ctactttagg ttgttcaact gttatattgc gtgttagcgc
       61 ttttcctgat gttcgtatgc cggacttaag aactggacaa gattatgcta cttggttaaa
      121 aatacttaaa agaggcacaa gagcatactt gcttcctgag gtattaacat cttatagaat
      181 tgtgaatggt tcaatatcaa ggaataaatt taaaaaagca ttaaggcaat gggaaattta
      241 tagaaaatat gaaaatttga gctttttata tagctgctat tgtttttccc tttatgcgtt
      301 cagagctgta gttaggtaag aaaatgaata taaatttaat tgaacttcaa cagcatggtg
      361 atgagagagg ggcattagta tctcttgagt gtaacaaaaa tatccccttc gat
//
LOCUS       DAZGAN020000469          410 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZGAN020000469 DAZGAN020000000
VERSION     DAZGAN020000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.476"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..233)
                     /locus_tag="ACHKYO_RS27565"
                     /old_locus_tag="pgaptmp_005513"
     CDS             complement(<1..233)
                     /locus_tag="ACHKYO_RS27565"
                     /old_locus_tag="pgaptmp_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HGU4432126.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASR"
     gene            complement(243..>410)
                     /locus_tag="ACHKYO_RS27570"
                     /old_locus_tag="pgaptmp_005514"
     CDS             complement(243..>410)
                     /locus_tag="ACHKYO_RS27570"
                     /old_locus_tag="pgaptmp_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HGU4432127.1"
                     /translation="LIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSV
                     LIYDLRCPTVL"
BASE COUNT           85 a          147 c           91 g           87 t
ORIGIN      
        1 cgtgacgctg ccgtgatttc ccgaccggac tgaccgcgca cccagatgcg gatttccgcc
       61 tgcgccattt ccgcaccgga ctgcatccgc tcccggctgc tcctgccacg gatatccgca
      121 tgaattttcc cgcatgacac ccattcttcc gtcatttctc cggcagcatt acgggttaac
      181 accgggttca gaacacttat catctgtgtc agacgacctg cagatattgc cattccccct
      241 cctcataaca ccgtcggaca acgcaaatcg taaatcagca cggaaacaga aaacggcagc
      301 tccccctgaa gcagttcttc ccgctccgca agatccggat tccggtacag catcccggtc
      361 agtcgcatgg cagccccctt catccgggtt aatgcctcgc ccgggatcag
//
LOCUS       DAZGAN020000470          407 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZGAN020000470 DAZGAN020000000
VERSION     DAZGAN020000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.477"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            17..>407
                     /locus_tag="ACHKYO_RS27575"
                     /old_locus_tag="pgaptmp_005515"
     CDS             17..>407
                     /locus_tag="ACHKYO_RS27575"
                     /old_locus_tag="pgaptmp_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432128.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKTHRAKIVEFD
                     KLDNDSQIDNDFGLHIIYFLQHGHWKVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNT
                     TDTFIFTIIPDNNHVLKLSSPITVTVEC"
BASE COUNT          145 a           69 c           65 g          128 t
ORIGIN      
        1 atattacagg ttatttatga aagtatttca caatatattt aagcacttct cttcaaacca
       61 tcaagataag cattcagata aagttaacag tcatcaacac catggcaagg ttgataaaac
      121 acatcgtgca aagatagttg aatttgataa actggataat gattcgcaaa tagataacga
      181 ttttggcctg catatcatct atttcctgca acatggtcac tggaaggtaa atgatcgtag
      241 tcaccagatg gagaaagtat ggttttataa tagtgaacct tctatagata ttcaagaata
      301 taatagattc gccgacaata ctactgatac gtttatcttt acaattatac cagacaataa
      361 ccatgtgtta aaattatcgt ctcccattac cgttactgtt gagtgta
//
LOCUS       DAZGAN020000471          402 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZGAN020000471 DAZGAN020000000
VERSION     DAZGAN020000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.478"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            31..279
                     /locus_tag="ACHKYO_RS27580"
                     /old_locus_tag="pgaptmp_005516"
     CDS             31..279
                     /locus_tag="ACHKYO_RS27580"
                     /old_locus_tag="pgaptmp_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4432129.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            281..>402
                     /locus_tag="ACHKYO_RS27585"
                     /old_locus_tag="pgaptmp_005517"
     CDS             281..>402
                     /locus_tag="ACHKYO_RS27585"
                     /old_locus_tag="pgaptmp_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432130.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSG"
BASE COUNT          111 a           86 c          104 g          101 t
ORIGIN      
        1 gcgcgggtaa ctttttagaa tagaggaagt atgagtagaa gagaagtttc agatcacgat
       61 tggccgcctg aaatcatcaa ggcgcgctta cacatggctg gcttatcact gcgctcttta
      121 tctttaaagg ctggttacag cagggattcg ctgaaaagcg tattacgtac tccttgccga
      181 ccgtatcagc aaataattgc tgatgctctt ggggtatcgc ctgaagaaat ctggcccagt
      241 cgataccagg ttaagagcta tatgagaaag gcgtcatgat atgtatgtca ttgcaaaaga
      301 actgattggc gcgcccggaa tgcctgctac aacaaaaggt attcgccagg cattacaacg
      361 ttacgtacaa gggaaaagct gttgttcccg tcgtcgctca gg
//
LOCUS       DAZGAN020000472          398 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZGAN020000472 DAZGAN020000000
VERSION     DAZGAN020000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.479"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>398)
                     /locus_tag="ACHKYO_RS27590"
                     /old_locus_tag="pgaptmp_005518"
     CDS             complement(<1..>398)
                     /locus_tag="ACHKYO_RS27590"
                     /old_locus_tag="pgaptmp_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432131.1"
                     /translation="LKSVLAVKLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRG
                     YIHSGEDDKFDNFMRQHRFSKVNFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQAL
                     RSTDDMIKANAWYLYKEWIRSDDVSPIFIET"
BASE COUNT          133 a           67 c           55 g          143 t
ORIGIN      
        1 gtttctataa atataggtga aacatcatca ctacgaatcc actctttata aagataccag
       61 gcatttgctt tgatcatatc atccgttgat cgtaaggcct gatcgataag ttggataaaa
      121 aaattgttat ttttatcaat cagcccttta ttcatattaa atctgttaat aaaatggatc
      181 atctgttggg tatcgaaatt caccttactg aatcgatgtt gacgcataaa attatcgaac
      241 ttgtcatctt caccagaatg tatataacca cgtataattg gatcttttat ataaggacaa
      301 tggctaaatg ttttcttcat attctcaata aagtttttat taacaatatt aagaatcttt
      361 aattgcatca ccagttttac tgccagaacg gatttcaa
//
LOCUS       DAZGAN020000473          391 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZGAN020000473 DAZGAN020000000
VERSION     DAZGAN020000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.480"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..84
                     /locus_tag="ACHKYO_RS27595"
                     /old_locus_tag="pgaptmp_005519"
     CDS             <1..84
                     /locus_tag="ACHKYO_RS27595"
                     /old_locus_tag="pgaptmp_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432132.1"
                     /translation="PVPDDVADRVRRAVCEIRACEAGADPR"
     gene            68..286
                     /locus_tag="ACHKYO_RS27600"
                     /old_locus_tag="pgaptmp_005520"
     CDS             68..286
                     /locus_tag="ACHKYO_RS27600"
                     /old_locus_tag="pgaptmp_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432133.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            231..>391
                     /locus_tag="ACHKYO_RS27605"
                     /old_locus_tag="pgaptmp_005521"
     CDS             231..>391
                     /locus_tag="ACHKYO_RS27605"
                     /old_locus_tag="pgaptmp_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU4432134.1"
                     /translation="MNLLIWPERLLFRQERRSKEKGMDDSDRAQAVMERGSERALCNR
                     LTRKRRAMDA"
BASE COUNT           81 a          100 c          128 g           82 t
ORIGIN      
        1 cctgttcctg atgatgtggc tgatcgggtg cgccgggccg tctgtgaaat acgtgcctgt
       61 gaagccggtg ccgatccccg ctgaatggct ggctgactgc ctggtccctc ctgcgccgga
      121 gccgttcacg tttggggcat cggtcactta caacctgcaa ctgctggcgg tgatcaagaa
      181 ctgcaacgtg gacaaggcca gtattcgtcg tctggaggcg cggcgacaac atgaatttac
      241 tgatatggcc ggaacgcctg ctgttccggc aggaaagacg aagtaaggaa aaaggtatgg
      301 atgattcaga tcgtgctcag gctgtgatgg agcggggatc agaacgcgcc ctttgtaacc
      361 ggctgacacg taagcgccgg gcaatggatg c
//
LOCUS       DAZGAN020000474          386 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZGAN020000474 DAZGAN020000000
VERSION     DAZGAN020000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.481"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>386
                     /locus_tag="ACHKYO_RS27610"
                     /old_locus_tag="pgaptmp_005522"
     CDS             <1..>386
                     /locus_tag="ACHKYO_RS27610"
                     /old_locus_tag="pgaptmp_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HGU4432135.1"
                     /translation="DNMRAVSVLNLGTIDEPGHADNTATLKLKKTAPFSALLSVNGER
                     NSQKSLAEWIEDWADYLVGFDANGDTIQATKAAAAVRKITIEANQTADFEDNDFSGKR
                     SLMESVEAKTKDIMPVAFEFKCVPFEG"
BASE COUNT          112 a           92 c           94 g           88 t
ORIGIN      
        1 gataatatgc gagccgtcag tgtgcttaac ctaggtacta ttgatgaacc aggtcacgca
       61 gataacaccg ccactctcaa actgaaaaag acagcaccgt tctctgccct gctgtctgtt
      121 aatggcgagc gtaactccca gaagtcactg gcagaatgga ttgaagactg ggccgactac
      181 cttgtgggct ttgatgctaa tggtgacacc attcaggcaa ccaaagcggc tgcggcagtc
      241 cgtaaaatca caattgaagc aaaccagacc gctgattttg aagacaatga cttcagcggc
      301 aaacgctctc tgatggagtc tgtcgaagcg aaaaccaaag acattatgcc agtggcattt
      361 gaatttaaat gcgttccgtt tgaagg
//
LOCUS       DAZGAN020000475          380 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZGAN020000475 DAZGAN020000000
VERSION     DAZGAN020000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.482"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>380
                     /locus_tag="ACHKYO_RS27615"
                     /old_locus_tag="pgaptmp_005523"
     CDS             <1..>380
                     /locus_tag="ACHKYO_RS27615"
                     /old_locus_tag="pgaptmp_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432136.1"
                     /translation="QLLNELDEQAKRQAVMNELKAKPRSELLEEYRRLSQKEGRTETE
                     EQQFQAIREVIRPQQEVTPEAQSQPENAEDGNGSIYPTVRFRDPNEVRIEINGNGASR
                     PAERIEKVRPDNRYFTDEKSAMGS"
BASE COUNT          115 a           86 c          120 g           59 t
ORIGIN      
        1 cagttgttga atgaactgga cgaacaggcg aagcgacagg ctgtgatgaa tgagctgaag
       61 gcgaagccgc gttctgaact gcttgaggaa taccgcagac tcagccagaa agaggggcgc
      121 accgagactg aagaacaaca gtttcaggca atacgagaag tcattcgccc acaacaggaa
      181 gtgacgccgg aagcacagtc acagcctgaa aatgcggagg atggtaacgg gagcatttac
      241 ccgacggtgc ggttccggga cccgaatgaa gtccgcattg aaattaacgg gaatggtgcg
      301 tccagaccag cggaacgcat tgaaaaggtg cgcccggaca accgttattt cacggatgag
      361 aaaagcgcca tggggagtga
//
LOCUS       DAZGAN020000476          375 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZGAN020000476 DAZGAN020000000
VERSION     DAZGAN020000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.483"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>375
                     /locus_tag="ACHKYO_RS27620"
                     /old_locus_tag="pgaptmp_005524"
     CDS             <1..>375
                     /locus_tag="ACHKYO_RS27620"
                     /old_locus_tag="pgaptmp_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HGU4432137.1"
                     /translation="ILDEAVWVKQALEKNQPGKPVRVINPDDESIRIFSWLADNFPDL
                     QYFKLQLLDASNLRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLES
                     QAIETLSAIGLSYEKYKTANNV"
BASE COUNT          135 a           60 c           72 g          108 t
ORIGIN      
        1 atattttgga tgaagcagtc tgggtcaaac aagcactgga aaaaaatcaa ccaggaaagc
       61 cagtacgggt gataaatcct gatgatgaat cgatacgaat tttttcatgg cttgctgata
      121 atttccctga tttacaatat tttaaacttc aactattaga tgccagtaat ctaagattga
      181 cagtgagtaa gcaacggaac gctatcacac agcaactaat cgataatctt attaaaggat
      241 tactgcaaac tatgccatat gccagcaata taagtattgc ggtattagat gataatgtat
      301 tggaaagtca ggctattgaa acgttgtcag cgataggtct ttcttatgaa aaatataaaa
      361 cagctaacaa tgtgt
//
LOCUS       DAZGAN020000477          371 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZGAN020000477 DAZGAN020000000
VERSION     DAZGAN020000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.484"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(195..>371)
                     /locus_tag="ACHKYO_RS27625"
                     /old_locus_tag="pgaptmp_005525"
     CDS             complement(195..>371)
                     /locus_tag="ACHKYO_RS27625"
                     /old_locus_tag="pgaptmp_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4432138.1"
                     /translation="RTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEACRRRLATD
                     ATHLRPASMTNNEK"
BASE COUNT           81 a           80 c           97 g          113 t
ORIGIN      
        1 gagaaggaca ggccgcgtaa aggcgtgaag aaaacgttta tctttagtcc ttctgcaccc
       61 tgattatttc atcagaaatt aattatcact ctgttgttat tctgtacatc ccggctgggt
      121 aagtcttgtt ccgccgggta tgaagatgaa atattgttgg aggacagtgg gtacctgctc
      181 ctgtaaccga acgttcattt ctcgttattt gtcatgctgg ccgggcgcag gtgcgttgca
      241 tctgttgcca gccttctccg gcaggcttca ataacccacg ctgaaaagtt accggaccct
      301 ttatgctcaa gggcgatgtt gatctgttca atcatgtgat tggggaaacg gatattgcgg
      361 gttgtggttc t
//
LOCUS       DAZGAN020000478          369 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZGAN020000478 DAZGAN020000000
VERSION     DAZGAN020000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.485"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>369
                     /locus_tag="ACHKYO_RS27630"
                     /old_locus_tag="pgaptmp_005526"
     CDS             <1..>369
                     /locus_tag="ACHKYO_RS27630"
                     /old_locus_tag="pgaptmp_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432139.1"
                     /translation="LSRKPQVTWYGWDGDRLTTIQNDRSRIQTIYQPGSFTPLIRVET
                     ATGELARTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL
                     ASCGLTVEQMQNQMDPVYTPA"
BASE COUNT           80 a          107 c          127 g           55 t
ORIGIN      
        1 gctgtcacgg aaaccgcaag tgacctggta cggctgggac ggcgaccggc tgaccacgat
       61 acagaacgac aggagccgca tccagacgat ttatcagccg gggagcttca caccactcat
      121 cagagttgaa actgccaccg gtgaactggc cagaacgcag cgccgcagcc tggcggatgc
      181 gcttcagcag tccggcggcg aagacggtgg cagtgtggtg ttcccgccgg tgctggtgca
      241 gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg gtgagtgagg aaagccgccg
      301 ctggctggca tcgtgcggcc tgaccgtgga gcagatgcaa aaccagatgg acccggtgta
      361 cacgccggc
//
LOCUS       DAZGAN020000479          368 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZGAN020000479 DAZGAN020000000
VERSION     DAZGAN020000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.486"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>368
                     /locus_tag="ACHKYO_RS27635"
                     /old_locus_tag="pgaptmp_005527"
     CDS             <1..>368
                     /locus_tag="ACHKYO_RS27635"
                     /old_locus_tag="pgaptmp_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4432140.1"
                     /translation="QPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAIR
                     RRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDLS
                     RLFSRLARTKQLRKAKKADVL"
BASE COUNT           76 a          101 c          113 g           78 t
ORIGIN      
        1 caaccaaaca cctggatgat caccctgtca cccgcgcact ccagcgtcac ggagtgtctg
       61 ctggagctgg ccgatgcgct ggggattgat tacacccgcg cgaacaaagg ggcattatcc
      121 cgcgccatcc gccgtcgcct gatgggaacg cgtggactgg tgattgtgga tgaggcggat
      181 catcttggta ttgacggtct ggagcaactc cgggcaattc aggacgccac ggggatcggg
      241 atggtgctta ttggtaaccc gcgcggattg tttaaaggtg gacgccgcgc ctttgatgat
      301 ttatcgcgcc tgttcagccg tcttgcccgt acaaaacaac ttcgcaaggc caaaaaggcg
      361 gatgtgct
//
LOCUS       DAZGAN020000480          365 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZGAN020000480 DAZGAN020000000
VERSION     DAZGAN020000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.487"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>365
                     /locus_tag="ACHKYO_RS27640"
                     /old_locus_tag="pgaptmp_005528"
     CDS             <1..>365
                     /locus_tag="ACHKYO_RS27640"
                     /old_locus_tag="pgaptmp_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4432141.1"
                     /translation="NQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQL
                     ACDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACV
                     ESFFHSLKVECIHGEHFISR"
BASE COUNT           86 a           86 c          111 g           82 t
ORIGIN      
        1 aaccagaagt gggcaggaga catcacgtac ttacgtacag atgaaggctg gctgtatctg
       61 gcagtggtca ttgacctgtg gtcacgtgcc gttattggct ggtcaatgtc gccacgcatg
      121 acggcgcaac tggcctgcga tgccctgcag atggcgctgt ggcggcgtaa gaggccccgg
      181 aacgttatcg ttcacacgga ccgtggaggc cagtactgtt cagcagatta tcaggcgcaa
      241 ctgaagcggc ataatctgcg tggaagtatg agcgcaaaag gttgctgcta cgataatgcc
      301 tgcgtggaaa gcttctttca ttcgctgaaa gtggaatgta tccatggaga acactttatc
      361 agccg
//
LOCUS       DAZGAN020000481          365 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZGAN020000481 DAZGAN020000000
VERSION     DAZGAN020000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.488"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            127..202
                     /locus_tag="ACHKYO_RS27645"
                     /old_locus_tag="pgaptmp_005529"
     tRNA            127..202
                     /locus_tag="ACHKYO_RS27645"
                     /old_locus_tag="pgaptmp_005529"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:161..163,aa:Glu,seq:ttc)
BASE COUNT          105 a           82 c           86 g           92 t
ORIGIN      
        1 gaaagtgaaa agcaaggcgt ttacgcgttg ggagtgaggc tgaagagaat aaggccgttc
       61 gctttctatt aatgaaagct caccctacac gaaaatatca cgcaacgcgt gataagcaat
      121 tttcgtgtcc ccttcgtcta gaggcccagg acaccgccct ttcacggcgg taacaggggt
      181 tcgaatcccc taggggacgc cacttgctgg tttgtgagtg aaagtcgccg accccaatat
      241 ctcaaaacag actgttaagt cttgtttgag atatttgctc tttaaaaatc tggatcaagc
      301 tgaaaattga aacactgaac aacgaaagtt gttcgtgagt ctctcaaatt ttcgcaactc
      361 tgaag
//
LOCUS       DAZGAN020000482          361 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZGAN020000482 DAZGAN020000000
VERSION     DAZGAN020000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.489"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(110..>361)
                     /locus_tag="ACHKYO_RS27650"
                     /old_locus_tag="pgaptmp_005530"
     CDS             complement(110..>361)
                     /locus_tag="ACHKYO_RS27650"
                     /old_locus_tag="pgaptmp_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4432142.1"
                     /translation="ERLPQHEDKLMTIAERLEQQGIQKGIQQGIQQGIQQGIQQGIQK
                     GRMEEKQDIARQLQKTGMPSAQICQITGLSEAELKNITH"
BASE COUNT           63 a           99 c           74 g          125 t
ORIGIN      
        1 gcaccttttt caaaaccttt ctgtataaga cgttctgcaa tcgtcatact gtttgctctg
       61 ctcctgctga caaaaaaacc cggcattttg ccgggctttg tggttctcat cagtgagtga
      121 tgttcttcag ttcagcttcg gaaagtccgg tgatctgaca gatttgtgca gatggcattc
      181 ccgttttctg aagctgacgc gcaatatcct gcttctcctc catgcggcct ttctgtattc
      241 cctgctgtat tccctgctgt attccctgct gtattccctg ctgtattcct ttctgtattc
      301 cttgctgctc aagacgttcg gcaatagtca tcagtttatc ctcatgctgc ggcagacgct
      361 c
//
LOCUS       DAZGAN020000483          350 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZGAN020000483 DAZGAN020000000
VERSION     DAZGAN020000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.490"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(35..259)
                     /locus_tag="ACHKYO_RS27655"
                     /old_locus_tag="pgaptmp_005531"
     CDS             complement(35..259)
                     /locus_tag="ACHKYO_RS27655"
                     /old_locus_tag="pgaptmp_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4432143.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
BASE COUNT           90 a          113 c           68 g           79 t
ORIGIN      
        1 gccggggtgt aatacgaaaa atttctgata tgcatcatgt ttccgtgccc tcctccgata
       61 ccggcaccca gaccggcatt ttttccgcac cgacgccccg cttcatacct gccggtgcag
      121 catccgctgc aaagacttta tacaccacat aatccaccac aataccgtct tccggccagc
      181 gccctgctgc ctcataaacc ggccgcaggg acaggggata aaacatattc tctgacggtg
      241 aaaaaacata ctgttccata caatgccctc agtatccgat cgcttcccat gaaaacttgc
      301 cgtagcatcc cctgactgtt atcagagtca cagacactaa tgacggattc
//
LOCUS       DAZGAN020000484          349 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZGAN020000484 DAZGAN020000000
VERSION     DAZGAN020000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.491"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>349
                     /locus_tag="ACHKYO_RS27660"
                     /old_locus_tag="pgaptmp_005532"
     CDS             <1..>349
                     /locus_tag="ACHKYO_RS27660"
                     /old_locus_tag="pgaptmp_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGU4432144.1"
                     /translation="SIKFLDEIIAYQQHKDDKYPVKEHTLTQLSIRVNKNMQDNINSL
                     MAEHGINDAMFMLMSLLSTSDNFCMSPSDISNVLQFSKTNVTRISDSLVTKGFIRRVD
                     SRNDRRSKYIHLTP"
BASE COUNT          125 a           60 c           57 g          107 t
ORIGIN      
        1 agcatcaaat ttcttgatga aataatagca tatcaacaac ataaagatga taaatatcct
       61 gtaaaagagc atacccttac tcaactctct attcgggtaa ataaaaatat gcaggataat
      121 ataaatagtc tgatggctga acatggaatc aatgatgcca tgtttatgct aatgtctcta
      181 ctttccactt cggataattt ctgtatgtct ccatcagaca tcagtaatgt gctgcaattc
      241 agcaaaacaa atgttaccag aattagcgat tctttagtta ccaaaggatt tataagacga
      301 gttgatagca ggaatgatcg tcgtagtaaa tatattcatt taactccgg
//
LOCUS       DAZGAN020000485          329 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZGAN020000485 DAZGAN020000000
VERSION     DAZGAN020000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.492"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>329
                     /locus_tag="ACHKYO_RS27665"
                     /old_locus_tag="pgaptmp_005533"
     CDS             <1..>329
                     /locus_tag="ACHKYO_RS27665"
                     /old_locus_tag="pgaptmp_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4432145.1"
                     /translation="QLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMV
                     ATLKIPMERRNRRTGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFR
                     HSYAMHML"
BASE COUNT           75 a           95 c          103 g           56 t
ORIGIN      
        1 cagcttgcga ccctgaaaca acggaccgaa aaagctgcca ggacggcggg gagaatgccc
       61 gccggtcagc agactcaccg gctggttccg ctctctgact cctggtacgt cagccagctg
      121 cagacgatgg tggcaacact gaaaataccc atggagcggc gtaaccgtcg cacaggaagg
      181 acagagaaag cgcggatctg ggaagtgacg gacagaacgg tcaggacctg gattggggag
      241 gcggttgccg ccgctgctgc tgacggtgtg acgttctctg ttccggtcac accacatacg
      301 ttccgccatt cctatgcgat gcacatgct
//
LOCUS       DAZGAN020000486          326 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZGAN020000486 DAZGAN020000000
VERSION     DAZGAN020000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.493"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>326)
                     /locus_tag="ACHKYO_RS27670"
                     /old_locus_tag="pgaptmp_005534"
     CDS             complement(<1..>326)
                     /locus_tag="ACHKYO_RS27670"
                     /old_locus_tag="pgaptmp_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4432146.1"
                     /translation="EEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAWRNNEANK
                     PKSRAGKSGAEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKTLTEIQKQ
                     ELLRNAA"
BASE COUNT           48 a          107 c           73 g           98 t
ORIGIN      
        1 gcagcattgc gcagcagctc ctgcttctgt atctccgtca gggttttcag ctcgccctgc
       61 gaggtctggt atttcagttt tgccagttct gtattcttcc cggccagtgc cagttgttcc
      121 ttctgctgct tcagcagacg ggaaaaactg tcttccgctt tttccgcccc tgattttccg
      181 gcccgggatt tgggtttatt cgcctcatta ttgcgccagg cttccatcga attacggatg
      241 taacgctgcc tcgcctcctg atacgtatcc cccaccagac caaggtcatc cgccgcatac
      301 cccagccggg cacgctcttt ttcttc
//
LOCUS       DAZGAN020000487          326 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZGAN020000487 DAZGAN020000000
VERSION     DAZGAN020000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.494"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..248)
                     /locus_tag="ACHKYO_RS27675"
                     /old_locus_tag="pgaptmp_005535"
     CDS             complement(<1..248)
                     /locus_tag="ACHKYO_RS27675"
                     /old_locus_tag="pgaptmp_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4432147.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDAN"
BASE COUNT          107 a           56 c           61 g          102 t
ORIGIN      
        1 tgatttgcat caccggattt aatctcaact tcattttcct gaggcgtcga tggattatcg
       61 atacggaata ctgtcgcctt gatgtactga acttcattac ttttagatat tacgcgaacg
      121 ttgccttcac cctgactatt aatggaaaca gccataggat aaacagtcat atttgactgt
      181 gccaaaaaag gagcgaacac gaataaaagc ggcgtaagcc gtgataagag attaattttc
      241 gccaccatat aaatttgatc ttacaatatt gtttctaaaa agttggtgta ttagagatgt
      301 agatttattg taagtcaata taaaaa
//
LOCUS       DAZGAN020000488          325 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZGAN020000488 DAZGAN020000000
VERSION     DAZGAN020000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.495"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>325)
                     /locus_tag="ACHKYO_RS27680"
                     /old_locus_tag="pgaptmp_005536"
     CDS             complement(<1..>325)
                     /locus_tag="ACHKYO_RS27680"
                     /old_locus_tag="pgaptmp_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4432148.1"
                     /translation="TLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAAAA
                     AREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQASQS
                     SDEFSS"
BASE COUNT           70 a          101 c           87 g           67 t
ORIGIN      
        1 gacgaaaatt catccgatga ctgcgacgcc tgtttcagac gggcattcac ggagctccat
       61 tcatccgcca gcgaaatcag gtgtccggtg gcaaaggcac cggcaaacgc accggtcatt
      121 ccgacagccg aagcgcggat ttccgtcaac tggctgttca gctcagccag agcccggcgc
      181 tgctccctgg ctgccgcagc agcctgacgt ccgccattct gcagggtccg gtaatattca
      241 ctgcccatac gggacgcccg ctggatctcc gactggaatg actgtgaatt tgccgaaatt
      301 ttgataatca gttcacgtaa cgtcg
//
LOCUS       DAZGAN020000489          324 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZGAN020000489 DAZGAN020000000
VERSION     DAZGAN020000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.496"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..302
                     /locus_tag="ACHKYO_RS27685"
                     /old_locus_tag="pgaptmp_005537"
     CDS             <1..302
                     /locus_tag="ACHKYO_RS27685"
                     /old_locus_tag="pgaptmp_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGU4432149.1"
                     /translation="EYERHRLTRAQADAQELKNARDSAEVVETAFCTFVLSRIAGEIA
                     SILDGIPLSVQRRFPELENRHVDFLKRDIIKAMNKAAALDELIPGLLSEYIEQSG"
BASE COUNT           80 a           80 c           93 g           71 t
ORIGIN      
        1 ttgagtacga acgccatcga cttacgcgtg cgcaggccga cgcacaggaa ctgaagaatg
       61 ccagagactc cgctgaagtg gtggaaaccg cattctgtac tttcgtgctg tcgcggatcg
      121 caggtgaaat tgccagtatt ctcgacggga tccccctgtc ggtgcagcgg cgttttccgg
      181 aactggaaaa ccgacatgtt gatttcctga aacgggatat catcaaagcc atgaacaaag
      241 cagccgcgct ggatgaactg ataccggggt tgctgagtga atatatcgaa cagtcaggtt
      301 aacaggctgc ggcattttgt ccgc
//
LOCUS       DAZGAN020000490          319 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZGAN020000490 DAZGAN020000000
VERSION     DAZGAN020000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.497"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..319
                     /gene="yqeJ"
                     /locus_tag="ACHKYO_RS27690"
                     /old_locus_tag="pgaptmp_005538"
                     /pseudo
     CDS             1..319
                     /gene="yqeJ"
                     /locus_tag="ACHKYO_RS27690"
                     /old_locus_tag="pgaptmp_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YqeJ"
BASE COUNT          123 a           45 c           62 g           89 t
ORIGIN      
        1 gggacgagca aatcttacca tcgactttat gtttaacaaa aaaataaaac aggaactgtt
       61 gcgttgagtg gaacctggca acaaggtaac agagaaagta aatcaatacg gcgaaatatt
      121 gaatacacat ggattgaaaa ctatgacaca gcccatttaa catcaaagaa agttaataag
      181 tttgagatta tggatcaagt tgatgatgat agacttgtac aacttattcc tgatttttat
      241 gtctttccag aaaaaagtgt aagctataat atactaaagc aaggtaagca tgcttttatt
      301 ttgagcattg gtaacagag
//
LOCUS       DAZGAN020000491          315 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZGAN020000491 DAZGAN020000000
VERSION     DAZGAN020000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.498"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>315)
                     /locus_tag="ACHKYO_RS27695"
                     /old_locus_tag="pgaptmp_005539"
     CDS             complement(<1..>315)
                     /locus_tag="ACHKYO_RS27695"
                     /old_locus_tag="pgaptmp_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HGU4432150.1"
                     /translation="DSEPLKALPQSNIWPVSIYYRLLSFDYFSARLDSLLYLDADIVC
                     KGSLNELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGSYFNSGVMYINLREWLK
                     QRL"
BASE COUNT          114 a           70 c           40 g           91 t
ORIGIN      
        1 tagtctttgt tttaaccatt cccgtaaatt aatatacatt acaccagagt taaaatagct
       61 accgttaaaa tcctcattac acaaacgctc agcgctttta ctttgcatag catctacatc
      121 aattaccact gccccatatt catctttaaa ctctaatgct attaactcgt tcaatgaacc
      181 cttacagacg atatcagcat caagatataa taatgaatcc aatcgcgcag aaaaataatc
      241 aaatgaaagc aaacgataat aaatagatac tggccagata tttgattgag gtagcgcctt
      301 caatggctca gaatc
//
LOCUS       DAZGAN020000492          307 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZGAN020000492 DAZGAN020000000
VERSION     DAZGAN020000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.499"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>307
                     /locus_tag="ACHKYO_RS27700"
                     /old_locus_tag="pgaptmp_005540"
     CDS             <1..>307
                     /locus_tag="ACHKYO_RS27700"
                     /old_locus_tag="pgaptmp_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432151.1"
                     /translation="AVENALRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGAL
                     MNSLYMFANASIQGTANLVRTLGHLNGEGPLLERLRWKNLNVPQKIALAAVGAGYLLG
                     "
BASE COUNT           68 a           77 c           92 g           70 t
ORIGIN      
        1 cgcggttgaa aacgcactgc gtctgtcagc ttataaacac gcccgtgatg ccggtttgtc
       61 acgccagcag gcggcgtctc ttgccaaaaa catgacggtg aactttaacc gtcgtggtga
      121 gcagggggcg ctgatgaact cgctgtacat gttcgccaac gccagtattc agggaacggc
      181 aaatctggtg agaacgctcg gacatcttaa tggtgaggga cctttactgg agcgccttcg
      241 ctggaagaat ctgaatgttc cgcagaaaat cgcgcttgcc gctgtgggag caggttatct
      301 gcttggc
//
LOCUS       DAZGAN020000493          304 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZGAN020000493 DAZGAN020000000
VERSION     DAZGAN020000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.500"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>304)
                     /locus_tag="ACHKYO_RS27705"
                     /old_locus_tag="pgaptmp_005541"
     CDS             complement(<1..>304)
                     /locus_tag="ACHKYO_RS27705"
                     /old_locus_tag="pgaptmp_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432152.1"
                     /translation="EYGLQQMLEGYRIILAREFFTPENRKSVPAHDESWVIICDALLG
                     RLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTMSEMVVMFGYV"
BASE COUNT           76 a          101 c           53 g           74 t
ORIGIN      
        1 tacacgtatc caaacatcac caccatctcg gacatcgtca gaggtcgcgc atacgtatta
       61 tgtacccact caccattccg gattttcctg tacgtctcca ggcgccccgt aacatcttcc
      121 gcatcaatct gaagataacg gcgtccctga aacatgcagc acatcagcct gccaaggagg
      181 gcatcacaga ttatcaccca tgactcatca tgtgccggta cactcttcct gttctctggt
      241 gtaaaaaatt ccctcgccag tattatcctg tacccctcaa gcatctgctg caggccatac
      301 tcca
//
LOCUS       DAZGAN020000494          299 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZGAN020000494 DAZGAN020000000
VERSION     DAZGAN020000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.501"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            173..>299
                     /locus_tag="ACHKYO_RS27710"
                     /old_locus_tag="pgaptmp_005542"
     CDS             173..>299
                     /locus_tag="ACHKYO_RS27710"
                     /old_locus_tag="pgaptmp_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGU4432153.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRY"
BASE COUNT           85 a           63 c           54 g           97 t
ORIGIN      
        1 atcactcaat aacttccatc gagatcgggt aataacattt gaacagatcg ctgaataaca
       61 tcgatggaga tcacttttga ctcattttgt tattcagtga tctccatcaa tgttattgga
      121 acttcacagg tgtgttgatc tgtatctttt gccattccgg taaaggatac ctatgccaac
      181 agttccaatt tccatgagaa aacttaaaga aattcttagg cttaaatacg gtgttggact
      241 cagccatcga caaattggtc gtagtcttgc aatctcccct tccgttgtat ccagatatg
//
LOCUS       DAZGAN020000495          299 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZGAN020000495 DAZGAN020000000
VERSION     DAZGAN020000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.502"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            41..>299
                     /locus_tag="ACHKYO_RS27715"
                     /old_locus_tag="pgaptmp_005543"
     CDS             41..>299
                     /locus_tag="ACHKYO_RS27715"
                     /old_locus_tag="pgaptmp_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4432154.1"
                     /translation="MNCFQFVCGCAFDNPIQRLIMLRVLMSGSSDGEGERVIDHQVLA
                     DFCCCSKQAIFRETLALERAGYLHIRKIATLTIDAKARLQPA"
BASE COUNT           69 a           57 c           81 g           92 t
ORIGIN      
        1 tttgctccgg aaaaatgttt aagcgtctgg gaggtgtggc gtgaactgtt ttcagtttgt
       61 gtgcggatgt gctttcgata acccgattca gcgcctgatt atgttgcgtg ttttgatgtc
      121 gggttcttca gacggtgaag gcgagagagt tattgatcat caggtgcttg ctgatttctg
      181 ctgttgttct aagcaagcga tattcaggga aaccctggca ctggaaagag ctggttatct
      241 tcatatccga aaaattgcaa cgcttactat tgatgcaaaa gccagactac aacctgcgc
//
LOCUS       DAZGAN020000496          296 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZGAN020000496 DAZGAN020000000
VERSION     DAZGAN020000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.503"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>296)
                     /locus_tag="ACHKYO_RS27720"
                     /old_locus_tag="pgaptmp_005544"
     CDS             complement(<1..>296)
                     /locus_tag="ACHKYO_RS27720"
                     /old_locus_tag="pgaptmp_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4432155.1"
                     /translation="LVPNQFALGIYWQLSSLPEGVTLSEEGNDMLMSFFLQTDATNLN
                     AMYHKKQNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPA"
BASE COUNT           65 a           90 c           71 g           70 t
ORIGIN      
        1 gccggagatt tcaccagtgt gaacggtgta ccccgtttct catccagtac cggcgtcacc
       61 tgaatgctgt tgtttccggc aaactcaaaa gccagtgtgt gccatccgtt atcaaagacc
      121 ccgaacgtat ccagcttcgc attctgcttc ttgtggtaca tcgcgttcag gttcgtcgca
      181 tccgtctgca ggaagaagga catcagcatg tcgttgcctt cctctgacag cgtcactccc
      241 tccggcaggg acgacaactg ccagtaaata cccagggcaa actgattcgg caccag
//
LOCUS       DAZGAN020000497          293 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZGAN020000497 DAZGAN020000000
VERSION     DAZGAN020000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.504"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..>293
                     /locus_tag="ACHKYO_RS27725"
                     /old_locus_tag="pgaptmp_005545"
     rRNA            1..>293
                     /locus_tag="ACHKYO_RS27725"
                     /old_locus_tag="pgaptmp_005545"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           86 a           64 c           86 g           57 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg tta
//
LOCUS       DAZGAN020000498          292 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZGAN020000498 DAZGAN020000000
VERSION     DAZGAN020000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.505"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..217)
                     /locus_tag="ACHKYO_RS27730"
                     /old_locus_tag="pgaptmp_005546"
     CDS             complement(<1..217)
                     /locus_tag="ACHKYO_RS27730"
                     /old_locus_tag="pgaptmp_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432156.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEG"
BASE COUNT           73 a           93 c           77 g           49 t
ORIGIN      
        1 gcccttcgct gacccggcgc accgtacact gccccaccgg gcgggaatgg tcatgctgcc
       61 agagaaacgg gatcgcactg ccaaactccg cgccctccgg ctccaggatg tcaccatccc
      121 gatccggaga aggcgttgac gcaatcccgg tgatcacccg ctcatcctca ctgaaggatt
      181 tcaccgtcag cagggagcag gcccgtttaa gagtcacatc agcctcctga aaataaaaaa
      241 accgccgcag cggtccatga tggttacagg gtgaacaggg ttatatgaaa aa
//
LOCUS       DAZGAN020000499          290 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZGAN020000499 DAZGAN020000000
VERSION     DAZGAN020000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.506"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..288)
                     /locus_tag="ACHKYO_RS27735"
                     /old_locus_tag="pgaptmp_005547"
     CDS             complement(<1..288)
                     /locus_tag="ACHKYO_RS27735"
                     /old_locus_tag="pgaptmp_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4432157.1"
                     /translation="MSESKSLSGRIYQDPEHNGEFELDRVLTDIYAPQLIIFLPDKPN
                     GTGILIIPGGGYRAVMLDKEGTALAPEFLSQGYTLFVLSYRLPSPAEEENIN"
BASE COUNT           93 a           62 c           50 g           85 t
ORIGIN      
        1 gtttatattt tcttcctcag ctggcgatgg taatctgtaa ctaagaacaa ataatgtata
       61 gccctgagat aaaaactctg gagcaagagc tgtaccttcc ttatctaaca taacagcccg
      121 gtagcccccc ccgggaataa tcaatattcc tgttccattt ggtttatctg gtaaaaaaat
      181 aattaactga ggagcataaa tatctgtcaa tacacgatca agttcaaatt ccccgttatg
      241 ctcaggatcc tgataaatac ggcctgaaag agatttactc tcagatattg
//
LOCUS       DAZGAN020000500          284 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZGAN020000500 DAZGAN020000000
VERSION     DAZGAN020000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.507"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..72)
                     /gene="yrhD"
                     /locus_tag="ACHKYO_RS27740"
                     /old_locus_tag="pgaptmp_005548"
     CDS             complement(<1..72)
                     /gene="yrhD"
                     /locus_tag="ACHKYO_RS27740"
                     /old_locus_tag="pgaptmp_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhD"
                     /protein_id="HGU4432158.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILA"
BASE COUNT           89 a           45 c           46 g          104 t
ORIGIN      
        1 ggctaaaata gagactacat gtcttcggtc catctcactt aaggagtgta gttccgttgt
       61 aagtttttcc atagcttgca ctgctaaatt tcgaacaagg aattttctgc tggtaatctc
      121 taaaaagatg gcatggttta caatgatttt tgtttccttt tgattattat gaacaactgt
      181 ccatgatttc gtttaagaat gaagagaaat cactaaacga actgaatata ttttctgtgc
      241 caatattatc tctaatttca aaaaagttac ttttaatgtc ataa
//
LOCUS       DAZGAN020000501          282 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZGAN020000501 DAZGAN020000000
VERSION     DAZGAN020000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.508"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>282
                     /locus_tag="ACHKYO_RS27745"
                     /old_locus_tag="pgaptmp_005549"
     CDS             <1..>282
                     /locus_tag="ACHKYO_RS27745"
                     /old_locus_tag="pgaptmp_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4432159.1"
                     /translation="RGGSAFTSGAEGAFSESSGATAESSLWGVGKPLYNDLLTRTKAA
                     LDKNPKNVLLSVVWMLGEFDLKSATYAQHPQLFLNMVNQFRADLSAYSSQ"
BASE COUNT           68 a           76 c           76 g           62 t
ORIGIN      
        1 cgtggagggt cagcatttac ttccggcgct gagggggcat tcagtgaatc gtcaggagcg
       61 acggccgaat catccctgtg gggtgtcggc aagccgctgt acaacgatct gcttacccgt
      121 acaaaagccg cgctggataa aaaccccaaa aacgtcctgt tatccgtggt ctggatgctg
      181 ggagagtttg atctgaagtc agcaacatac gcacagcacc cgcaattatt cctgaacatg
      241 gtgaatcagt tccgcgccga tctcagcgca tattcttcac ag
//
LOCUS       DAZGAN020000502          281 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZGAN020000502 DAZGAN020000000
VERSION     DAZGAN020000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.509"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT           93 a           50 c           66 g           72 t
ORIGIN      
        1 atatctttaa ctctccatgc ccgtgatgga ctggaacaga taatatacct gactttaagc
       61 gcaacagagt ttatgcatct gaatagcaga aacatgaaac ctctgtgtgt ggaaaaaatg
      121 gaaaggcagg caaaaaacat ctgctcagaa aattgttgtg gcaatgtctt ttgcagactg
      181 agattctgca tgacattatg taacagagcg atgtgagaga aggttgtttt aatgaacgga
      241 acagaaacag cactatcaga gtgctggatc ctgtcggcac t
//
LOCUS       DAZGAN020000503          280 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZGAN020000503 DAZGAN020000000
VERSION     DAZGAN020000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.510"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT           74 a           51 c           59 g           96 t
ORIGIN      
        1 ctcctcctca tcagttcgta gcagagtgta ctcagcatgg caagttcagc ttctgatgat
       61 ttacagtgtg tattcaggag ggtaagaaat tgttcaaaaa catcagggtt tatactggtg
      121 ctttgatatt cgttactcat agtatttcct ttcaggtcat tattacttcc ggatttaaca
      181 tgatgtctgt aaccataaaa taatgaacgt gttccgggag tccttgccgt gtagtttccc
      241 gtaaaaatac tgttgtgcat tacatgtcat taggtggaaa
//
LOCUS       DAZGAN020000504          278 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZGAN020000504 DAZGAN020000000
VERSION     DAZGAN020000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.511"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            179..>278
                     /locus_tag="ACHKYO_RS27750"
                     /old_locus_tag="pgaptmp_005550"
     CDS             179..>278
                     /locus_tag="ACHKYO_RS27750"
                     /old_locus_tag="pgaptmp_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432160.1"
                     /translation="MNISYVNSNKTTSLPVELDALNNKDISYAKDFS"
BASE COUNT          101 a           46 c           43 g           88 t
ORIGIN      
        1 tgatgaatcc tctgcaaatg agcgaaattt gatagaacat ttatctgctg tacgatttaa
       61 tgaacttaat gatacagcat tacaaaaaca atcgaagttt ataaagatga ttcctgattg
      121 accagaaccc tatcctacaa tggtgactaa ttttattact cccgatggag atgatgatat
      181 gaatatatca tatgtgaata gcaacaaaac gacatcttta cctgttgagc tagatgcact
      241 aaataataaa gatatctctt atgccaaaga tttttctt
//
LOCUS       DAZGAN020000505          272 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZGAN020000505 DAZGAN020000000
VERSION     DAZGAN020000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.512"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..130)
                     /locus_tag="ACHKYO_RS27755"
                     /old_locus_tag="pgaptmp_005551"
     CDS             complement(<1..130)
                     /locus_tag="ACHKYO_RS27755"
                     /old_locus_tag="pgaptmp_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU4432161.1"
                     /translation="MSNKYCQELVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGP"
     gene            complement(130..>272)
                     /locus_tag="ACHKYO_RS27760"
                     /old_locus_tag="pgaptmp_005552"
     CDS             complement(130..>272)
                     /locus_tag="ACHKYO_RS27760"
                     /old_locus_tag="pgaptmp_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432162.1"
                     /translation="MAAFRESHSQPERNNFLARRERCLRKSSKRAVSGDEWYLSGNYV
                     GA"
BASE COUNT           57 a           85 c           59 g           71 t
ORIGIN      
        1 atgggccaaa cagggtgtta attccccaga aaatgttatc cggcgtgcgc cactgatcgc
       61 ccacttcctt cagttcatgg gctggtttgt tccgcagctc caccagctcc tggcaatatt
      121 tattactcat taagccccca cgtaattccc tgacagatac cactcatcac ccgatacagc
      181 gcgcttgctg cttttccgta agcaccgctc acgacgtgcc agaaaattgt ttcgttctgg
      241 ctgggagtgg ctttcacgga atgccgccat cc
//
LOCUS       DAZGAN020000506          271 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZGAN020000506 DAZGAN020000000
VERSION     DAZGAN020000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.513"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(42..271)
                     /gene="ybfQ"
                     /locus_tag="ACHKYO_RS27765"
                     /old_locus_tag="pgaptmp_005553"
                     /pseudo
     CDS             complement(42..271)
                     /gene="ybfQ"
                     /locus_tag="ACHKYO_RS27765"
                     /old_locus_tag="pgaptmp_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT           88 a           58 c           42 g           83 t
ORIGIN      
        1 ttatttagta caaaaaagta gcgttacgtg atctttccct gggatacaac tctggcaatg
       61 gtatcgtgaa caggaatacc attttcaaaa tcaccatatt gcttcaaaaa atcgagatgt
      121 gtttccccga aatcctctat atcttcccaa ccttctgcac cagaaataac ggcacaaata
      181 gtcaacagta gaatatctga caatttatgc accactttcc aggcttgtct gtaatcgggt
      241 gtaatagaaa tatgttccat caatttttta a
//
LOCUS       DAZGAN020000507          254 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZGAN020000507 DAZGAN020000000
VERSION     DAZGAN020000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.514"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..91)
                     /locus_tag="ACHKYO_RS27770"
                     /old_locus_tag="pgaptmp_005554"
     CDS             complement(<1..91)
                     /locus_tag="ACHKYO_RS27770"
                     /old_locus_tag="pgaptmp_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU4432163.1"
                     /translation="MKWIVIDTIIQPSCGISFSAIWGNMKMIIW"
BASE COUNT           90 a           40 c           33 g           91 t
ORIGIN      
        1 accagatgat cattttcata ttaccccata tggctgaaaa agatatgccg catgaaggtt
       61 gaattatcgt gtcaattact atccacttca tttgttatgt cttatcccac ggtatttaat
      121 atagttcatt tggatgttca tttctttatt ttgcatatga gtatattacc ccttcaaaaa
      181 ataaattaat taaaacgatt gcttatataa aacaaaattt aaagcaagga atctcaatgg
      241 atgttaaaca aaat
//
LOCUS       DAZGAN020000508          254 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZGAN020000508 DAZGAN020000000
VERSION     DAZGAN020000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.515"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>254)
                     /locus_tag="ACHKYO_RS27775"
                     /old_locus_tag="pgaptmp_005555"
     CDS             complement(<1..>254)
                     /locus_tag="ACHKYO_RS27775"
                     /old_locus_tag="pgaptmp_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4432164.1"
                     /translation="VVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSG
                     RSLRFACSPGEVSVLYAVAPKAGRSQIPDELLTWPEEVAA"
BASE COUNT           64 a           72 c           62 g           56 t
ORIGIN      
        1 cgcagccact tcttcaggcc atgtgaggag ttcatccggt atctggctgc gtccggcttt
       61 cggagcgacg gcataaagca cgctcacctc accgggagaa caggcaaatc gcagggagcg
      121 tccggagctg atatccacat cccgaccgac aaaaagctca tgattatcgt cagagatacg
      181 gatgatatga acgcactcct catcatcttt gtcatacgga agcacgattt cttttcctgc
      241 tactggtaca acag
//
LOCUS       DAZGAN020000509          250 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZGAN020000509 DAZGAN020000000
VERSION     DAZGAN020000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.516"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..117
                     /locus_tag="ACHKYO_RS27780"
                     /old_locus_tag="pgaptmp_005556"
     CDS             <1..117
                     /locus_tag="ACHKYO_RS27780"
                     /old_locus_tag="pgaptmp_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4432165.1"
                     /translation="WGGSLWHCNSMTGDKPGEAHSSAWTLAAKRGRDAGGGK"
     gene            114..>250
                     /locus_tag="ACHKYO_RS27785"
                     /old_locus_tag="pgaptmp_005557"
     CDS             114..>250
                     /locus_tag="ACHKYO_RS27785"
                     /old_locus_tag="pgaptmp_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4432166.1"
                     /translation="MTVLLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     D"
BASE COUNT           60 a           60 c           83 g           47 t
ORIGIN      
        1 tgggggggct cgctgtggca ctgcaacagt atgaccggtg ataaacccgg agaagctcat
       61 tcatcagcct ggaccctggc tgcaaaacgt gggcgggatg ctggaggcgg aaaatgacgg
      121 tattactgac actggaagag atcaaggcac atctgcgtgt cgaccatgac gcggatgatg
      181 acatgctgat ggacaaggtt cgtcaggcta ccgccgtgct gctggcctac attcagggca
      241 gccgggataa
//
LOCUS       DAZGAN020000510          247 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZGAN020000510 DAZGAN020000000
VERSION     DAZGAN020000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.517"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>247)
                     /locus_tag="ACHKYO_RS27790"
                     /old_locus_tag="pgaptmp_005558"
     CDS             complement(<1..>247)
                     /locus_tag="ACHKYO_RS27790"
                     /old_locus_tag="pgaptmp_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HGU4432167.1"
                     /translation="LPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSLFIDEMAHP
                     LQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGK"
BASE COUNT           67 a           64 c           63 g           53 t
ORIGIN      
        1 tccttaccgg acgtcgaggt gtaccagtca ttccactgac gcggcgtgac gtacaggacg
       61 taatatggat cttctccgtg aagttcatca ccggacagac gaaccggctg taacggatgc
      121 gccatttcgt caatgaacag ggagagattg tccaccaggc caatagaaaa aatatctgcc
      181 gcttcaatct gctcaaagct tgtcgcatca ccgccaaaaa agtgacggtc atgtgtcgga
      241 ggcagta
//
LOCUS       DAZGAN020000511          246 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZGAN020000511 DAZGAN020000000
VERSION     DAZGAN020000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.518"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>246
                     /locus_tag="ACHKYO_RS27795"
                     /old_locus_tag="pgaptmp_005559"
     CDS             <1..>246
                     /locus_tag="ACHKYO_RS27795"
                     /old_locus_tag="pgaptmp_005559"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HGU4432168.1"
                     /translation="QMRKISVSYICKSLFLYCSASLPVLSANGIPDKMIVELGDNSAA
                     KANFAVAVGQYSQANAEKSISLGASARAIENNSTALG"
BASE COUNT           74 a           44 c           48 g           80 t
ORIGIN      
        1 ttcaaatgcg taaaatcagt gtcagttata tatgcaaatc attatttctc tactgctcag
       61 cttctttacc tgtcttatct gcaaatggta ttcctgataa aatgatcgtg gaacttggtg
      121 ataacagtgc ggcaaaagct aatttcgctg ttgctgttgg tcaatattca caggcaaatg
      181 cagaaaaaag tatttcttta ggtgcatcag caagagctat tgaaaataac tctaccgcgt
      241 taggtg
//
LOCUS       DAZGAN020000512          244 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZGAN020000512 DAZGAN020000000
VERSION     DAZGAN020000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.denovo.519"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>244
                     /locus_tag="ACHKYO_RS27800"
                     /old_locus_tag="pgaptmp_005560"
     CDS             <1..>244
                     /locus_tag="ACHKYO_RS27800"
                     /old_locus_tag="pgaptmp_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU4432169.1"
                     /translation="PMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVR
                     ELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILEL"
BASE COUNT           55 a           60 c           74 g           55 t
ORIGIN      
        1 gcccgatgcc gcagactcgt gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca
       61 tcatcgtgtt cctgaacaaa tgcgacatgg ttgatgacga agagctgctg gaactggttg
      121 aaatggaagt tcgtgaactt ctgtctcagt acgacttccc gggcgacgac actccgatcg
      181 ttcgtggttc tgctctgaaa gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg
      241 aact
//
LOCUS       DAZGAN020000513         5425 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.guided.205, whole genome shotgun sequence.
ACCESSION   DAZGAN020000513 DAZGAN020000000
VERSION     DAZGAN020000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.guided.205"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1104
                     /locus_tag="ACHKYO_RS27805"
                     /old_locus_tag="pgaptmp_005561"
     CDS             <1..1104
                     /locus_tag="ACHKYO_RS27805"
                     /old_locus_tag="pgaptmp_005561"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU4432170.1"
                     /translation="ALYSCSTFAAPQQINDIVHRTITPLIEQQKIPGMAVAVIYQGKP
                     YYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLSDPTTKYWPEL
                     TAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQRLYANSS
                     IGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGYREGKAVHVS
                     PGALDAKTYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYWQTGDMYQGLG
                     WEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGATGGFGSYVAF
                     IPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1193..1726
                     /gene="blc"
                     /locus_tag="ACHKYO_RS27810"
                     /old_locus_tag="pgaptmp_005562"
     CDS             1193..1726
                     /gene="blc"
                     /locus_tag="ACHKYO_RS27810"
                     /old_locus_tag="pgaptmp_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU4432171.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1723..2040)
                     /gene="sugE"
                     /locus_tag="ACHKYO_RS27815"
                     /old_locus_tag="pgaptmp_005563"
     CDS             complement(1723..2040)
                     /gene="sugE"
                     /locus_tag="ACHKYO_RS27815"
                     /old_locus_tag="pgaptmp_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU4432172.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2215..2361)
                     /gene="ecnB"
                     /locus_tag="ACHKYO_RS27820"
                     /old_locus_tag="pgaptmp_005564"
     CDS             complement(2215..2361)
                     /gene="ecnB"
                     /locus_tag="ACHKYO_RS27820"
                     /old_locus_tag="pgaptmp_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU4432173.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2472..2597)
                     /gene="ecnA"
                     /locus_tag="ACHKYO_RS27825"
                     /old_locus_tag="pgaptmp_005565"
     CDS             complement(2472..2597)
                     /gene="ecnA"
                     /locus_tag="ACHKYO_RS27825"
                     /old_locus_tag="pgaptmp_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGU4432174.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2649..3215)
                     /gene="efp"
                     /locus_tag="ACHKYO_RS27830"
                     /old_locus_tag="pgaptmp_005566"
     CDS             complement(2649..3215)
                     /gene="efp"
                     /locus_tag="ACHKYO_RS27830"
                     /old_locus_tag="pgaptmp_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGU4432175.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3257..4285
                     /gene="epmB"
                     /locus_tag="ACHKYO_RS27835"
                     /old_locus_tag="pgaptmp_005567"
     CDS             3257..4285
                     /gene="epmB"
                     /locus_tag="ACHKYO_RS27835"
                     /old_locus_tag="pgaptmp_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGU4432176.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAKTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            4675..>5425
                     /gene="yjeJ"
                     /locus_tag="ACHKYO_RS27840"
                     /old_locus_tag="pgaptmp_005568"
     CDS             4675..>5425
                     /gene="yjeJ"
                     /locus_tag="ACHKYO_RS27840"
                     /old_locus_tag="pgaptmp_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HGU4432177.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQ"
BASE COUNT         1450 a         1325 c         1327 g         1323 t
ORIGIN      
        1 gcgctatact cttgctccac atttgctgcc cctcaacaaa tcaacgatat tgtgcatcgc
       61 acaattaccc cgcttataga gcaacaaaag atccccggta tggcggtggc ggtaatttat
      121 cagggtaaac cttattactt tacctggggc tatgcggaca tcgccaaaaa gcagcccgtc
      181 acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa catttacggg cgtgcttggt
      241 ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc ccacaacaaa atactggcct
      301 gaacttaccg ctaaacagtg gaatgggatc acactattac atctcgcaac ctacactgct
      361 ggcggcctgc cattgcaggt gccggatgag gtgaaatcct caagcgactt gctgcgcttc
      421 tatcaaaact ggcagcctgc atgggcgcca ggaacacaac gtctgtatgc caactccagt
      481 atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt tgagttttga gcaggcgatg
      541 caaacacgtg tcttccagcc actcaaactc aaccatacgt ggattaatgt accgcccgca
      601 gaagaaaaga attacgcctg gggatatcgc gagggcaagg cagttcatgt ttcgccaggg
      661 gcgttagatg ctaaaactta tggtgtgaag tcgaccattg aagatatggc ctgctgggta
      721 cgaagcaata tgaatccccg tgatatcaac gacaaaacac ttcagcaagg gatacaactg
      781 gcacaatctc gctactggca aaccggcgat atgtatcagg gcctgggctg ggaaatgctg
      841 gactggccgg taaatcctga cagcatcatt aacggcagtg gcaataaaat tgcactggca
      901 gcacaccccg taaaagcgat tacgccccca actcctgcag tacgcgcatc atgggtacat
      961 aaaacagggg caaccggcgg atttggtagc tatgtcgcgt ttattccaga aaaagagctg
     1021 ggtatagtga tgctggctaa caaaaactat cccaatccag cgagagtcgc cgccgcctgg
     1081 cagattctca acgctctaca gtaaaatttc atcgggtccg aattttcgga ccttttctgc
     1141 gcttttcctt gctgtcatct acacttagaa aaaaaccagt aaggaaacat ttatgcgcct
     1201 gctccctctc gttgccgcag cgacagctgc atttctggtc gttgcctgca gttctcctac
     1261 gccgccgcgt ggcgtaacag tagtgaataa tttcgacgcc aaacgttatc tcggtacctg
     1321 gtatgagatt gcccgttttg atcaccgttt tgaacgtgga ctggaaaaag tcaccgcaac
     1381 atacagcctg cgtgatgacg gcggcctgaa tgtcattaat aaaggctata accctgacag
     1441 aggaatgtgg cagcagagtg aagggaaagc gtactttacc ggcgcaccaa ctcgcgctgc
     1501 gctgaaagtg tcattctttg gtcctttcta tggcggttat aacgttattg cactcgatcg
     1561 ggaataccgc catgcgctgg tttgcggccc ggaccgcgac tacctgtgga tactctcccg
     1621 cacgccaacc atttctgacg aagtgaaaca ggagatgctg gcagtcgcga cccgggaagg
     1681 gtttgatgtc agtaagttta tttgggtaca gcagcccggt agttagtgag tgctgagttt
     1741 cagaccaata atccccaata cgattaacgc cagactcgcc aggcgcatcg ggttagcgga
     1801 ctcaccgagc agcacaatgc cggtgatagc cgcgccgact gcgccaatac ccgtccacac
     1861 ggcataagcc gtccctactg gtaacgattt catcgcccag gcaagtagcg ccatactgac
     1921 tatcatcgcc gtcacggtaa taacactcgg cgtcaaacgg ctaaagccgt gggtatattt
     1981 caggccaacg gcccatacca cttccagcag accagcaata actaagataa tccaggacat
     2041 atcaggctcc ggagaattgg ggccgtcccc ggtgaaaaga ggcgtttgca ggtcgtcctg
     2101 caaagctgat ggttgaacgg atattttgcc aggtggcagg ggaatttcaa ctattacctt
     2161 tgtgactatc gcaaaaaaac aaacggcacg acataactgt cgtaccgtta ttgcttattg
     2221 ctgcgctttc gttgctgcac cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac
     2281 gccacgcgtg gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc
     2341 tgcaattgtc ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta
     2401 tagcaaatta ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa
     2461 gagaagaaaa attagctggc agcgcgggag atggagttgc cgagatgttt gatgtcttcg
     2521 ccgaaaccgc gagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg
     2581 ataaggcgtt tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca
     2641 caaccgcatt acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact
     2701 tcgccgattt gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt
     2761 ttgccgccag tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt
     2821 tcaacgaagt tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac
     2881 tctgcctgat ccagcagcca tttagcgttg tcgccaattg cttttgcatc agcagacagc
     2941 tgctcgaaag tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc
     3001 aggttcatat cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct
     3061 acgcgagtac cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc
     3121 ggttttacga attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga
     3181 ccagcacgaa aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg
     3241 gtagctaagc cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg
     3301 gttaacgcaa cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat
     3361 agacgcggac gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt
     3421 gccccgctca tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca
     3481 ggtacttacc tcgcaagatg agtttgtcgt cgcgcccgga ttctccaccg acccactgga
     3541 agaacagcac agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct
     3601 ggtcaaaggc ggctgcgcgg taaattgccg ttattgcttc cgtcgccact tcccctatgc
     3661 cgaaaatcag ggcaacaagc gtaactggca gactgctctt gagtatgttg ctgctcatcc
     3721 ggaactggac gagatgattt tctccggcgg cgatccgctg atggcgaaag atcacgagct
     3781 ggactggttg ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag
     3841 ccgtctgccg attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg
     3901 ttctacgctg caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac
     3961 gttccgtcag gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt
     4021 tctgttacgt ggtgtgaacg ataacgcaaa aacgctggca aacctgagta atgcgttgtt
     4081 cgatgccggc gtaatgccgt attacctgca tgtgctcgat aaagtacagg gcgcggcgca
     4141 ttttatggtg agtgatgacg aagcacggca gattatgcgt gagttgctga cactggtgtc
     4201 gggttatctg gtgccgaaac tggcgcgaga aatcggcggc gaacccagca aaacgccgct
     4261 ggatctccag ctacgccagc agtaactgat taaacaggga agttaaacat tagcctgatg
     4321 cgctacgctt atcaggccta cattgtgcat tgaaattgaa tggatttgca atgaactgta
     4381 ggccggataa ggcgtttacg ccgcatccgg caagagcaac aaacgcaagt acagcaacct
     4441 gtcattaaac tgggaaattt aatctttgcc cgttgcataa cctttaatta ttcatataca
     4501 ttgctgttgt tactctttat atagccaaca ccaataatta acctttcttt tctgtttata
     4561 tagagtgagt acggctattt atgttatttt aacaactatt actaccaagc ccatttatct
     4621 ggcttataaa ttaaaattaa ttatttaatt gagaaatggt tagggagaac ctacatggcc
     4681 ataagcatca agggtgttaa taccggtgtt attcgcaaga gtaataattt cattgcactg
     4741 gcgctgaaaa ttaaagaacc tcgcaataaa gaatcgttgt tctttatgtc tgtcatggaa
     4801 ctgcgcgacc tgctgattgc gctggaaagt cgtatgcatc aaaagcataa actggatgct
     4861 gctgcacgcc ttcagtatga gcaagctcgc gataaagtca ttaagaaaat ggcagaaaac
     4921 atcccagaaa ttctggttga tgagcttaaa aatgccgata ttaatcgtcg ggttaacacg
     4981 ctggaactta ccgataatca aggcgaaaat cttaccttcg tattaacgct acatgacggt
     5041 agcaaatgtg agttagtcgt caatgagttg cagattgaaa tgctggcgcg agcaatcatt
     5101 catgcaatta ataatgcgga aatgcgtgaa ctggcattac gtattacctc actgttagat
     5161 ttcctgccgt tatatgacgt tgactgccag gaaaatggca atctggaata tgacacctat
     5221 tcccaaccgg aatggaaaca taacctgttc gaccattatc tggcggtgct ttaccgcttt
     5281 aaagatgaaa gtggcaaaga acaatttagc ggcgcagtgg tgaaaacgcg tgaagcaacg
     5341 ccgggtaaag aaatagaggc gatcactcgc cgaatgcttg atttcagcca acgactgaaa
     5401 aaactcgcgg gtgtgccatg tcagg
//
LOCUS       DAZGAN020000514         3637 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.guided.232, whole genome shotgun sequence.
ACCESSION   DAZGAN020000514 DAZGAN020000000
VERSION     DAZGAN020000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.guided.232"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1286)
                     /gene="pepD"
                     /locus_tag="ACHKYO_RS27845"
                     /old_locus_tag="pgaptmp_005569"
     CDS             complement(<1..1286)
                     /gene="pepD"
                     /locus_tag="ACHKYO_RS27845"
                     /old_locus_tag="pgaptmp_005569"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU4432178.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQII"
     gene            1547..2005
                     /gene="gpt"
                     /locus_tag="ACHKYO_RS27850"
                     /old_locus_tag="pgaptmp_005570"
     CDS             1547..2005
                     /gene="gpt"
                     /locus_tag="ACHKYO_RS27850"
                     /old_locus_tag="pgaptmp_005570"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU4432179.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2097..3341
                     /gene="frsA"
                     /locus_tag="ACHKYO_RS27855"
                     /old_locus_tag="pgaptmp_005571"
     CDS             2097..3341
                     /gene="frsA"
                     /locus_tag="ACHKYO_RS27855"
                     /old_locus_tag="pgaptmp_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU4432180.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            3399..>3637
                     /gene="crl"
                     /locus_tag="ACHKYO_RS27860"
                     /old_locus_tag="pgaptmp_005572"
     CDS             3399..>3637
                     /gene="crl"
                     /locus_tag="ACHKYO_RS27860"
                     /old_locus_tag="pgaptmp_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU4432181.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAG"
BASE COUNT          861 a          915 c          944 g          917 t
ORIGIN      
        1 tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc acgtaccaga
       61 tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt cgcttcggtt
      121 ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc tttaccgctg
      181 tcgatcagtg aacggatcag gcagtgaatt tctacgttat tgtcagtcat ggtcaccaca
      241 ccgacgttca gggaggtttc gaccacacct tttgccacgt cggaattgcg gatcacaccg
      301 ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat cagggcagct
      361 ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc tgccagctcg
      421 tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac tttatcagct
      481 gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt gccgccgttg
      541 aaatcgataa ggcgtagatc cagttcttcc gcatgacccg ccaggaagcg caccagtagt
      601 ttgttggcat tacccaggcc aacgtggatt tcaccgccgg agtgaccgcc tttcagacct
      661 tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc taaatgcagg
      721 ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc ttcttcggag
      781 tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa cgcaccgtcc
      841 ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac cacgttttcg
      901 tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc cagcgtggtg
      961 ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt cgtgaagtca
     1021 tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa gacgaccggt
     1081 ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc tacctgatcg
     1141 cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc gagttgctct
     1201 tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca cagcggctgt
     1261 ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc gcaaaacagt
     1321 attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt gcgagatatg
     1381 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
     1441 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
     1501 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
     1561 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
     1621 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac cgggtgcgtt
     1681 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     1741 cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg aaggcttcat
     1801 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     1861 aaaagcacat tttgtgacta tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     1921 tgttgttgat atcccgcaag atacctggat cgaacagccg tgggatatgg gcgtcgtatt
     1981 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     2041 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     2101 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     2161 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggct cttgatggta
     2221 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     2281 acccacgtga aatcctcgac gtccaggcac gtattgtgat gagcgatgct gaacgtaccg
     2341 atgatgattt atacgatacc gtgattggct accgtggtgg caactggatt tatgagtggg
     2401 ccacccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg caactcagtg
     2461 gtcgacactg gctgcatgcg gctacgttgt acaacattgc cgcctatcct catctgaaag
     2521 gagatgacct ggccgagcaa gcgcaggctt tatcaaaccg cgcctatgaa gaggccgccc
     2581 agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt gcgcccatca
     2641 ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta atgtgtggtg
     2701 gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt gcgccgcgcg
     2761 gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcttcaaaa tggaagctca
     2821 cccaggactc cagcctgttg catcagcacg tcttaaaggc gctgcctaac gtaccgtggg
     2881 tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt gccgtgcgtc
     2941 tggcatacct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg gtagttcata
     3001 ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt gacgttctgg
     3061 cgagtcgttt ggggatgcat gatgcttccg atgacgcgtt gcgcgtggag ctgaatcgct
     3121 attcattaaa agtgcaagga ttgctgggac gtcgctgccc aacgccaatg ttatcaggct
     3181 actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc tcatcatctg
     3241 ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt gacaaaggtc
     3301 ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct aaattttgcc
     3361 aatttggtaa aacagttgca tcacaacagg agatagcaat gacgttaccg agtggacacc
     3421 cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt gaaggtaagt
     3481 gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg aaaccggcac
     3541 cggaagtgcg tgaattctgg ggctggtgga tggagctgga agcgcaggaa tcccgtttta
     3601 cctacagcta ccagtttggt ctgttcgata aagcagg
//
LOCUS       DAZGAN020000515         1639 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.guided.300, whole genome shotgun sequence.
ACCESSION   DAZGAN020000515 DAZGAN020000000
VERSION     DAZGAN020000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.guided.300"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            268..1215
                     /gene="stxA1a"
                     /locus_tag="ACHKYO_RS27865"
                     /old_locus_tag="pgaptmp_005573"
     CDS             268..1215
                     /gene="stxA1a"
                     /locus_tag="ACHKYO_RS27865"
                     /old_locus_tag="pgaptmp_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HGU4432182.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRGRGITHNKILWDSSTLGAILMRRTISS"
     gene            1225..1494
                     /gene="stxB1a"
                     /locus_tag="ACHKYO_RS27870"
                     /old_locus_tag="pgaptmp_005574"
     CDS             1225..1494
                     /gene="stxB1a"
                     /locus_tag="ACHKYO_RS27870"
                     /old_locus_tag="pgaptmp_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HGU4432183.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          439 a          280 c          397 g          523 t
ORIGIN      
        1 tctacgagtt tgccagcctc ccccagtggc tggctttttt atgtccgtaa catcctgtgt
       61 atcaataaat gttgttgtct acgtacgtca agtagtcgca tgagatctga ccagatatgt
      121 taaggttgca gctctctttg aatataatta tcattttcat tacgttattg ttacgtttat
      181 ccggtgcgcc gtaaaacgcc gtccttcagg gggtggagga tgtcaagaat atagttatcg
      241 tatggtgctc aaggagtatt gtgtaatatg aaaataatta tttttagagt gctaactttt
      301 ttctttgtta tcttttcagt taatgtggtt gcgaaggaat ttaccttaga cttctcgact
      361 gcaaagacgt atgtagattc gctgaatgtc attcgctctg caataggtac tccattacag
      421 actatttcat caggaggtac gtctttactg atgattgata gtggcacagg ggataatttg
      481 tttgcagttg atgtcagagg gatagatcca gaggaagggc ggtttaataa tctacggctt
      541 attgttgaac gaaataattt atatgtgaca ggatttgtta acaggacaaa taatgttttt
      601 tatcgctttg ctgatttttc acatgttacc tttccaggta caacagcggt tacattgtct
      661 ggtgacagta gctataccac gttacagcgt gttgcaggga tcagtcgtac ggggatgcag
      721 ataaatcgcc attcgttgac tacttcttat ctggatttaa tgtcgcatag tggaacctca
      781 ctgacgcagt ctgtggcaag agcgatgtta cggtttgtta ctgttacagc tgaagcttta
      841 cgttttcggc aaatacagag gggatttcgt acaacactgg atgatctcag tgggcgttct
      901 tatgtaatga ctgctgaaga tgttgatctt acattgaact ggggaaggtt gagtagtgtc
      961 ctgcctgact atcatggaca agactctgtt cgtgtaggaa gaatttcttt tggaagcatt
     1021 aatgcaattc tgggaagcgt ggcattaata ctgaattgtc atcatcatgc atcgcgagtt
     1081 gccagaatgg catctgatga gtttccttct atgtgtccgg cagatggaag aggccgtggg
     1141 attacgcaca ataaaatatt gtgggattca tccactctgg gggcaattct gatgcgcaga
     1201 actattagca gttgaggggg taaaatgaaa aaaacattat taatagctgc atcgctttca
     1261 tttttttcag caagtgcgct ggcgacgcct gattgtgtaa ctggaaaggt ggagtataca
     1321 aaatataatg atgacgatac ctttacagtt aaagtgggtg ataaagaatt atttaccaac
     1381 agatggaatc ttcagtctct tcttctcagt gcgcaaatta cggggatgac tgtaaccatt
     1441 aaaactaatg cctgtcataa tggaggggga ttcagcgaag ttatttttcg ttgactcaga
     1501 atagctcagt gaaaatagca ggcggagatt cataaatgtt aaatacatct caattcagtc
     1561 agttgttgcc ggtctgataa tagatgtgtt agaaaatttc tgcatggtga atccccctgt
     1621 gcggaggggc gactggtga
//
LOCUS       DAZGAN020000516         1319 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.guided.322, whole genome shotgun sequence.
ACCESSION   DAZGAN020000516 DAZGAN020000000
VERSION     DAZGAN020000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.guided.322"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            75..1034
                     /gene="stxA2b"
                     /locus_tag="ACHKYO_RS27875"
                     /old_locus_tag="pgaptmp_005575"
     CDS             75..1034
                     /gene="stxA2b"
                     /locus_tag="ACHKYO_RS27875"
                     /old_locus_tag="pgaptmp_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HGU4432184.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNCIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1047..1310
                     /gene="stxB2b"
                     /locus_tag="ACHKYO_RS27880"
                     /old_locus_tag="pgaptmp_005576"
     CDS             1047..1310
                     /gene="stxB2b"
                     /locus_tag="ACHKYO_RS27880"
                     /old_locus_tag="pgaptmp_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4432185.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          370 a          264 c          312 g          373 t
ORIGIN      
        1 cgcttttgcg ggcctttttt atatctgcgc cgggtctggt atcgtattac ttcagccaaa
       61 aggaatacct gtatatgaag tgtatattgt taaaatgggt actgtgcctg ttactgggct
      121 tttcttcggt atcctattcc cgggaattta cgatagactt ttcgactcaa caaagttatg
      181 tctcttcgtt aaattgtata cggacagaaa tatcgacccc acttgaacat atatctcagg
      241 ggaccacatc ggtatctgtt attaaccaca ccccaccggg cagttatttt gctgtggata
      301 tacgagggct tgatgtctat caggcgcgtt ttgaccatct tcgtctgatt attgagcaaa
      361 ataatttata tgtggccgga ttcgttaata cggcaacaaa tactttctac agattttcag
      421 attttgcaca tatatcagtg cccggtgtga caactgtttc catgacaacg gacagcagtt
      481 ataccactct gcaacgtgtc gcagcgctgg aacgttccgg aatgcaaatc agtcgtcact
      541 cactggtttc atcatatctg gcgttaatgg agtttagtgg aaatgccatg accagagatg
      601 catccagagc agttctgcgt tttgtcactg tcacagcaga agccttacgg ttcaggcaaa
      661 tacagagaga atttcgtctg gcactgtctg aaactgctcc tgtttatacg atgacaccgg
      721 aagaagtgga cctcacactg aactggggga gaatcagcaa tgtgcttccg gagtttcggg
      781 gagagggtgg tgtcagagtg gggcgaatat cctttaataa tatatcagcg atactgggca
      841 cagtggcggt tatactgaat tgccatcatc agggggcacg ttccgttcgc gccgtgaatg
      901 aagagataca accagaatgt cagataactg gcgacaggcc agttataagg ataaacaata
      961 ctttatggga aagtaatacc gcagctgctt ttctgaatcg cagggctcac tctttaaata
     1021 catccggaga ataacaggag ttaaatatga agaagatatt tgtagcggct ttatttgctt
     1081 ttgtttctgt taatgcaatg gcagctgatt gtgcaaaagg taaaattgag ttctctaagt
     1141 ataatgagaa tgatacattc acagtaaaag tggccgggaa agagtactgg actaaccgct
     1201 ggaatctgca accgctactg caaagcgcac agttaacagg aatgacggta acaatcaaat
     1261 caaatacctg tgcgtcaggt tcaggatttg ctgaagtgca gtttaattaa tatcagaag
//
LOCUS       DAZGAN020000517         1319 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.guided.323, whole genome shotgun sequence.
ACCESSION   DAZGAN020000517 DAZGAN020000000
VERSION     DAZGAN020000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.guided.323"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            75..1034
                     /gene="stxA2b"
                     /locus_tag="ACHKYO_RS27885"
                     /old_locus_tag="pgaptmp_005577"
     CDS             75..1034
                     /gene="stxA2b"
                     /locus_tag="ACHKYO_RS27885"
                     /old_locus_tag="pgaptmp_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HGU4432186.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNCIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRSVNE
                     EIQPECQITGDRPVIRLNNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1047..1310
                     /gene="stxB2b"
                     /locus_tag="ACHKYO_RS27890"
                     /old_locus_tag="pgaptmp_005578"
     CDS             1047..1310
                     /gene="stxB2b"
                     /locus_tag="ACHKYO_RS27890"
                     /old_locus_tag="pgaptmp_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4432187.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          368 a          265 c          312 g          374 t
ORIGIN      
        1 cgcttttgcg ggcctttttt atatctgcgc cgggtctggt atcgtattac ttcagccaaa
       61 aggaatacct gtatatgaag tgtatattgt taaaatgggt actgtgcctg ttactgggct
      121 tttcttcggt atcctattcc cgggaattta cgatagactt ttcgactcaa caaagttatg
      181 tctcttcgtt aaattgtata cggacagaaa tatcgacccc acttgaacat atatctcagg
      241 ggaccacatc ggtatctgtt attaaccaca ccccaccggg cagttatttt gctgtggata
      301 tacgagggct tgatgtctat caggcgcgtt ttgaccatct tcgtctgatt attgagcaaa
      361 ataatttata tgtggccgga ttcgttaata cggcaacaaa tactttctac agattttcag
      421 attttgcaca tatatcagtg cccggtgtga caactgtttc catgacaacg gacagcagtt
      481 ataccactct gcaacgtgtc gcagcgctgg aacgttccgg aatgcaaatc agtcgtcact
      541 cactggtttc atcatatctg gcgttaatgg agtttagtgg aaatgccatg accagagatg
      601 catccagagc agttctgcgt tttgtcactg tcacagcaga agccttacgg ttcaggcaaa
      661 tacagagaga atttcgtctg gcactgtctg aaactgctcc tgtttatacg atgacaccgg
      721 aagaagtgga cctcacactg aactggggga gaatcagcaa tgtgcttccg gagtttcggg
      781 gagagggtgg tgtcagagtg gggcgaatat cctttaataa tatatcagcg atactgggca
      841 cagtggcggt tatactgaat tgccatcatc agggggcacg ttccgttcgc tccgtgaatg
      901 aagagataca accagaatgt cagataactg gcgacaggcc agttataagg ctaaacaata
      961 ctttatggga aagtaatacc gcagctgctt ttctgaatcg cagggctcac tctttaaata
     1021 catccggaga ataacgggag ttaaatatga agaagatatt tgtagcggct ttatttgctt
     1081 ttgtttctgt taatgcaatg gcagctgatt gtgcaaaagg taaaattgag ttctctaagt
     1141 ataatgagaa tgatacattc acagtaaaag tggccgggaa agagtactgg actaaccgct
     1201 ggaatctgca accgctactg caaagcgcac agttaacagg aatgacggta acaatcaaat
     1261 caaatacctg tgcgtcaggt tcaggatttg ctgaagtgca gtttaattaa tatcagaag
//
LOCUS       DAZGAN020000518         1319 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.guided.324, whole genome shotgun sequence.
ACCESSION   DAZGAN020000518 DAZGAN020000000
VERSION     DAZGAN020000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.guided.324"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            75..1034
                     /gene="stxA2b"
                     /locus_tag="ACHKYO_RS27895"
                     /old_locus_tag="pgaptmp_005579"
     CDS             75..1034
                     /gene="stxA2b"
                     /locus_tag="ACHKYO_RS27895"
                     /old_locus_tag="pgaptmp_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HGU4432188.1"
                     /translation="MKCILLKWILCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIVEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1047..1310
                     /gene="stxB2b"
                     /locus_tag="ACHKYO_RS27900"
                     /old_locus_tag="pgaptmp_005580"
     CDS             1047..1310
                     /gene="stxB2b"
                     /locus_tag="ACHKYO_RS27900"
                     /old_locus_tag="pgaptmp_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4432189.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          365 a          263 c          316 g          375 t
ORIGIN      
        1 cgcttttgcg ggcctttttt atatctgcgc cgggtctggt gccgtattac ttcagccaaa
       61 aggaacacct gtatatgaag tgtatattgt taaaatggat actgtgcctg ttgctgggct
      121 tttcttcggt atcctattcc cgggaattta cgatagactt ttcgactcaa caaagttatg
      181 tctcttcgtt aaatagtata cggacagaaa tatcgactcc tcttgagcat atatctcagg
      241 ggaccacatc ggtgtctgtt attaaccata ccccaccggg cagttatttt gctgtggata
      301 tacgagggct tgatgtctat caggcgcgtt ttgaccatct tcgtctgatt gttgagcaaa
      361 ataatttata tgtggccgga ttcgttaata cggcaacaaa tactttctac agattttctg
      421 attttgcaca tatatcagtg cccggtgtga caactgtttc catgacaacg gacagcagtt
      481 ataccactct gcaacgtgtc gcagcgctgg aacgttccgg aatgcaaatc agtcgtcact
      541 cactggtttc atcatatctg gcgttaatgg agtttagtgg aaatgccatg accagagatg
      601 catccagagc agttttgcgt tttgtcactg tcacagcaga agccttacgg ttcaggcaaa
      661 tacagagaga atttcgtctg gcactgtctg aaactgctcc tgtttatacg atgacaccgg
      721 aagaagtgga cctcacactg aactggggga gaatcagcaa tgtgcttccg gagtttcggg
      781 gagagggtgg tgtcagagtg gggcgaatat cctttaataa tatatcagcg atactgggca
      841 cagtggcggt tatactgaat tgccatcatc agggggcacg ttccgttcgc gccgtgaatg
      901 aagagataca accagaatgt cagataactg gcgacaggcc agttataagg ataaacaata
      961 ctttatggga aagtaatacc gcagctgctt ttctgaatcg cagggctcac tctttaaata
     1021 catccggaga ataacaggag ttaaatatga agaagatatt tgtagcggct ttatttgctt
     1081 ttgtttctgt taatgcaatg gcagctgatt gtgcaaaagg taaaattgag ttctctaagt
     1141 ataatgagaa tgatacattc acagtaaaag tggccgggaa agagtactgg actaaccgct
     1201 ggaatctgca accgctactg caaagcgcac agttaacagg aatgacggta acaatcaaat
     1261 caaatacctg tgcgtcaggt tcaggatttg ctgaagtgca gtttaattaa tatcagaag
//
LOCUS       DAZGAN020000519         1319 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2301M17788
            SAMN43283618-rid25106883.guided.325, whole genome shotgun sequence.
ACCESSION   DAZGAN020000519 DAZGAN020000000
VERSION     DAZGAN020000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283618
            Sequence Read Archive: SRR30325128
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351390.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351390.2
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:16:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,582
            CDSs (total)                      :: 5,488
            Genes (coding)                    :: 5,279
            CDSs (with protein)               :: 5,279
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 103 of 209
            Pseudo Genes (incomplete)         :: 103 of 209
            Pseudo Genes (internal stop)      :: 51 of 209
            Pseudo Genes (multiple problems)  :: 42 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283618-rid25106883.guided.325"
                     /serotype="O 91:H14"
                     /isolate="2301M17788"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            75..1034
                     /gene="stxA2b"
                     /locus_tag="ACHKYO_RS27905"
                     /old_locus_tag="pgaptmp_005581"
     CDS             75..1034
                     /gene="stxA2b"
                     /locus_tag="ACHKYO_RS27905"
                     /old_locus_tag="pgaptmp_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HGU4432190.1"
                     /translation="MKCILLKWILCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIVEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRSVNE
                     EIQPECQITGDRPVIRLNNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1047..1310
                     /gene="stxB2b"
                     /locus_tag="ACHKYO_RS27910"
                     /old_locus_tag="pgaptmp_005582"
     CDS             1047..1310
                     /gene="stxB2b"
                     /locus_tag="ACHKYO_RS27910"
                     /old_locus_tag="pgaptmp_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4432191.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          363 a          264 c          316 g          376 t
ORIGIN      
        1 cgcttttgcg ggcctttttt atatctgcgc cgggtctggt gccgtattac ttcagccaaa
       61 aggaacacct gtatatgaag tgtatattgt taaaatggat actgtgcctg ttgctgggct
      121 tttcttcggt atcctattcc cgggaattta cgatagactt ttcgactcaa caaagttatg
      181 tctcttcgtt aaatagtata cggacagaaa tatcgactcc tcttgagcat atatctcagg
      241 ggaccacatc ggtgtctgtt attaaccata ccccaccggg cagttatttt gctgtggata
      301 tacgagggct tgatgtctat caggcgcgtt ttgaccatct tcgtctgatt gttgagcaaa
      361 ataatttata tgtggccgga ttcgttaata cggcaacaaa tactttctac agattttctg
      421 attttgcaca tatatcagtg cccggtgtga caactgtttc catgacaacg gacagcagtt
      481 ataccactct gcaacgtgtc gcagcgctgg aacgttccgg aatgcaaatc agtcgtcact
      541 cactggtttc atcatatctg gcgttaatgg agtttagtgg aaatgccatg accagagatg
      601 catccagagc agttttgcgt tttgtcactg tcacagcaga agccttacgg ttcaggcaaa
      661 tacagagaga atttcgtctg gcactgtctg aaactgctcc tgtttatacg atgacaccgg
      721 aagaagtgga cctcacactg aactggggga gaatcagcaa tgtgcttccg gagtttcggg
      781 gagagggtgg tgtcagagtg gggcgaatat cctttaataa tatatcagcg atactgggca
      841 cagtggcggt tatactgaat tgccatcatc agggggcacg ttccgttcgc tccgtgaatg
      901 aagagataca accagaatgt cagataactg gcgacaggcc agttataagg ctaaacaata
      961 ctttatggga aagtaatacc gcagctgctt ttctgaatcg cagggctcac tctttaaata
     1021 catccggaga ataacgggag ttaaatatga agaagatatt tgtagcggct ttatttgctt
     1081 ttgtttctgt taatgcaatg gcagctgatt gtgcaaaagg taaaattgag ttctctaagt
     1141 ataatgagaa tgatacattc acagtaaaag tggccgggaa agagtactgg actaaccgct
     1201 ggaatctgca accgctactg caaagcgcac agttaacagg aatgacggta acaatcaaat
     1261 caaatacctg tgcgtcaggt tcaggatttg ctgaagtgca gtttaattaa tatcagaag
//
